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Sample records for two-component sensor system

  1. Phosphatase activity tunes two-component system sensor detection threshold.

    Science.gov (United States)

    Landry, Brian P; Palanki, Rohan; Dyulgyarov, Nikola; Hartsough, Lucas A; Tabor, Jeffrey J

    2018-04-12

    Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways in biology, and a major source of sensors for biotechnology. However, the input concentrations to which biosensors respond are often mismatched with application requirements. Here, we utilize a mathematical model to show that TCS detection thresholds increase with the phosphatase activity of the sensor histidine kinase. We experimentally validate this result in engineered Bacillus subtilis nitrate and E. coli aspartate TCS sensors by tuning their detection threshold up to two orders of magnitude. We go on to apply our TCS tuning method to recently described tetrathionate and thiosulfate sensors by mutating a widely conserved residue previously shown to impact phosphatase activity. Finally, we apply TCS tuning to engineer B. subtilis to sense and report a wide range of fertilizer concentrations in soil. This work will enable the engineering of tailor-made biosensors for diverse synthetic biology applications.

  2. Conductivity of two-component systems

    Energy Technology Data Exchange (ETDEWEB)

    Kuijper, A. de; Hofman, J.P.; Waal, J.A. de [Shell Research BV, Rijswijk (Netherlands). Koninklijke/Shell Exploratie en Productie Lab.; Sandor, R.K.J. [Shell International Petroleum Maatschappij, The Hague (Netherlands)

    1996-01-01

    The authors present measurements and computer simulation results on the electrical conductivity of nonconducting grains embedded in a conductive brine host. The shapes of the grains ranged from prolate-ellipsoidal (with an axis ratio of 5:1) through spherical to oblate-ellipsoidal (with an axis ratio of 1:5). The conductivity was studied as a function of porosity and packing, and Archie`s cementation exponent was found to depend on porosity. They used spatially regular and random configurations with aligned and nonaligned packings. The experimental results agree well with the computer simulation data. This data set will enable extensive tests of models for calculating the anisotropic conductivity of two-component systems.

  3. Regulation of natural competence by the orphan two-component system sensor kinase ChiS involves a non-canonical transmembrane regulator in Vibrio cholerae.

    Science.gov (United States)

    Yamamoto, Shouji; Mitobe, Jiro; Ishikawa, Takahiko; Wai, Sun Nyunt; Ohnishi, Makoto; Watanabe, Haruo; Izumiya, Hidemasa

    2014-01-01

    In Vibrio cholerae, 41 chitin-inducible genes, including the genes involved in natural competence for DNA uptake, are governed by the orphan two-component system (TCS) sensor kinase ChiS. However, the mechanism by which ChiS controls the expression of these genes is currently unknown. Here, we report the involvement of a novel transcription factor termed 'TfoS' in this process. TfoS is a transmembrane protein that contains a large periplasmic domain and a cytoplasmic AraC-type DNA-binding domain, but lacks TCS signature domains. Inactivation of tfoS abolished natural competence as well as transcription of the tfoR gene encoding a chitin-induced small RNA essential for competence gene expression. A TfoS fragment containing the DNA-binding domain specifically bound to and activated transcription from the tfoR promoter. Intracellular TfoS levels were unaffected by disruption of chiS and coexpression of TfoS and ChiS in Escherichia coli recovered transcription of the chromosomally integrated tfoR::lacZ gene, suggesting that TfoS is post-translationally modulated by ChiS during transcriptional activation; however, this regulation persisted when the canonical phosphorelay residues of ChiS were mutated. The results presented here suggest that ChiS operates a chitin-induced non-canonical signal transduction cascade through TfoS, leading to transcriptional activation of tfoR. © 2013 John Wiley & Sons Ltd.

  4. Structural characterization of the heme-based oxygen sensor, AfGcHK, its interactions with the cognate response regulator, and their combined mechanism of action in a bacterial two-component signaling system

    Czech Academy of Sciences Publication Activity Database

    Stráňava, M.; Martínek, V.; Man, Petr; Fojtíková, V.; Kavan, Daniel; Vaněk, O.; Shimizu, T.; Martínková, M.

    2016-01-01

    Roč. 84, č. 10 (2016), s. 1375-1389 ISSN 1097-0134 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:61388971 Keywords : heme-based oxygen sensor * histidine kinase * two-component signal transduction system Subject RIV: CE - Biochemistry

  5. Light Responsive Two-Component Supramolecular Hydrogel: A Sensitive Platform for Humidity Sensors

    KAUST Repository

    Samai, Suman

    2016-02-15

    The supramolecular assembly of anionic azobenzene dicarboxylate and cationic cetyltrimethylammonium bromide (CTAB) formed a stimuli responsive hydrogel with a critical gelation concentration (CGC) of 0.33 wt%. This self-sustainable two-component system was able to repair damage upon light irradiation. Moreover, it was successfully employed in the fabrication of highly sensitive humidity sensors for the first time.

  6. Light Responsive Two-Component Supramolecular Hydrogel: A Sensitive Platform for Humidity Sensors

    KAUST Repository

    Samai, Suman; Sapsanis, Christos; Patil, Sachin; Ezzeddine, Alaa; Moosa, Basem; Omran, Hesham; Emwas, Abdul-Hamid M.; Salama, Khaled N.; Khashab, Niveen M.

    2016-01-01

    The supramolecular assembly of anionic azobenzene dicarboxylate and cationic cetyltrimethylammonium bromide (CTAB) formed a stimuli responsive hydrogel with a critical gelation concentration (CGC) of 0.33 wt%. This self-sustainable two-component system was able to repair damage upon light irradiation. Moreover, it was successfully employed in the fabrication of highly sensitive humidity sensors for the first time.

  7. Polar localization of a tripartite complex of the two-component system DcuS/DcuR and the transporter DctA in Escherichia coli depends on the sensor kinase DcuS.

    Directory of Open Access Journals (Sweden)

    Patrick D Scheu

    Full Text Available The C4-dicarboxylate responsive sensor kinase DcuS of the DcuS/DcuR two-component system of E. coli is membrane-bound and reveals a polar localization. DcuS uses the C4-dicarboxylate transporter DctA as a co-regulator forming DctA/DcuS sensor units. Here it is shown by fluorescence microscopy with fusion proteins that DcuS has a dynamic and preferential polar localization, even at very low expression levels. Single assemblies of DcuS had high mobility in fast time lapse acquisitions, and fast recovery in FRAP experiments, excluding polar accumulation due to aggregation. DctA and DcuR fused to derivatives of the YFP protein are dispersed in the membrane or in the cytosol, respectively, when expressed without DcuS, but co-localize with DcuS when co-expressed at appropriate levels. Thus, DcuS is required for location of DctA and DcuR at the poles and formation of tripartite DctA/DcuS/DcuR sensor/regulator complexes. Vice versa, DctA, DcuR and the alternative succinate transporter DauA were not essential for polar localization of DcuS, suggesting that the polar trapping occurs by DcuS. Cardiolipin, the high curvature at the cell poles, and the cytoskeletal protein MreB were not required for polar localization. In contrast, polar localization of DcuS required the presence of the cytoplasmic PAS(C and the kinase domains of DcuS.

  8. Chloroplast two-component systems: evolution of the link between photosynthesis and gene expression.

    Science.gov (United States)

    Puthiyaveetil, Sujith; Allen, John F

    2009-06-22

    Two-component signal transduction, consisting of sensor kinases and response regulators, is the predominant signalling mechanism in bacteria. This signalling system originated in prokaryotes and has spread throughout the eukaryotic domain of life through endosymbiotic, lateral gene transfer from the bacterial ancestors and early evolutionary precursors of eukaryotic, cytoplasmic, bioenergetic organelles-chloroplasts and mitochondria. Until recently, it was thought that two-component systems inherited from an ancestral cyanobacterial symbiont are no longer present in chloroplasts. Recent research now shows that two-component systems have survived in chloroplasts as products of both chloroplast and nuclear genes. Comparative genomic analysis of photosynthetic eukaryotes shows a lineage-specific distribution of chloroplast two-component systems. The components and the systems they comprise have homologues in extant cyanobacterial lineages, indicating their ancient cyanobacterial origin. Sequence and functional characteristics of chloroplast two-component systems point to their fundamental role in linking photosynthesis with gene expression. We propose that two-component systems provide a coupling between photosynthesis and gene expression that serves to retain genes in chloroplasts, thus providing the basis of cytoplasmic, non-Mendelian inheritance of plastid-associated characters. We discuss the role of this coupling in the chronobiology of cells and in the dialogue between nuclear and cytoplasmic genetic systems.

  9. Comparative Analysis of Wolbachia Genomes Reveals Streamlining and Divergence of Minimalist Two-Component Systems

    Science.gov (United States)

    Christensen, Steen; Serbus, Laura Renee

    2015-01-01

    Two-component regulatory systems are commonly used by bacteria to coordinate intracellular responses with environmental cues. These systems are composed of functional protein pairs consisting of a sensor histidine kinase and cognate response regulator. In contrast to the well-studied Caulobacter crescentus system, which carries dozens of these pairs, the streamlined bacterial endosymbiont Wolbachia pipientis encodes only two pairs: CckA/CtrA and PleC/PleD. Here, we used bioinformatic tools to compare characterized two-component system relays from C. crescentus, the related Anaplasmataceae species Anaplasma phagocytophilum and Ehrlichia chaffeensis, and 12 sequenced Wolbachia strains. We found the core protein pairs and a subset of interacting partners to be highly conserved within Wolbachia and these other Anaplasmataceae. Genes involved in two-component signaling were positioned differently within the various Wolbachia genomes, whereas the local context of each gene was conserved. Unlike Anaplasma and Ehrlichia, Wolbachia two-component genes were more consistently found clustered with metabolic genes. The domain architecture and key functional residues standard for two-component system proteins were well-conserved in Wolbachia, although residues that specify cognate pairing diverged substantially from other Anaplasmataceae. These findings indicate that Wolbachia two-component signaling pairs share considerable functional overlap with other α-proteobacterial systems, whereas their divergence suggests the potential for regulatory differences and cross-talk. PMID:25809075

  10. Zero-range approximation for two-component boson systems

    International Nuclear Information System (INIS)

    Sogo, T.; Fedorov, D.V.; Jensen, A.S.

    2005-01-01

    The hyperspherical adiabatic expansion method is combined with the zero-range approximation to derive angular Faddeev-like equations for two-component boson systems. The angular eigenvalues are solutions to a transcendental equation obtained as a vanishing determinant of a 3 x 3 matrix. The eigenfunctions are linear combinations of Jacobi functions of argument proportional to the distance between pairs of particles. We investigate numerically the influence of two-body correlations on the eigenvalue spectrum, the eigenfunctions and the effective hyperradial potential. Correlations decrease or increase the distance between pairs for effectively attractive or repulsive interactions, respectively. New structures appear for non-identical components. Fingerprints can be found in the nodal structure of the density distributions of the condensates. (author)

  11. Global existence and blow-up phenomena for two-component Degasperis-Procesi system and two-component b-family system

    OpenAIRE

    Liu, Jingjing; Yin, Zhaoyang

    2014-01-01

    This paper is concerned with global existence and blow-up phenomena for two-component Degasperis-Procesi system and two-component b-family system. The strategy relies on our observation on new conservative quantities of these systems. Several new global existence results and a new blowup result of strong solutions to the two-component Degasperis- Procesi system and the two-component b-family system are presented by using these new conservative quantities.

  12. Two component systems: physiological effect of a third component.

    Directory of Open Access Journals (Sweden)

    Baldiri Salvado

    Full Text Available Signal transduction systems mediate the response and adaptation of organisms to environmental changes. In prokaryotes, this signal transduction is often done through Two Component Systems (TCS. These TCS are phosphotransfer protein cascades, and in their prototypical form they are composed by a kinase that senses the environmental signals (SK and by a response regulator (RR that regulates the cellular response. This basic motif can be modified by the addition of a third protein that interacts either with the SK or the RR in a way that could change the dynamic response of the TCS module. In this work we aim at understanding the effect of such an additional protein (which we call "third component" on the functional properties of a prototypical TCS. To do so we build mathematical models of TCS with alternative designs for their interaction with that third component. These mathematical models are analyzed in order to identify the differences in dynamic behavior inherent to each design, with respect to functionally relevant properties such as sensitivity to changes in either the parameter values or the molecular concentrations, temporal responsiveness, possibility of multiple steady states, or stochastic fluctuations in the system. The differences are then correlated to the physiological requirements that impinge on the functioning of the TCS. This analysis sheds light on both, the dynamic behavior of synthetically designed TCS, and the conditions under which natural selection might favor each of the designs. We find that a third component that modulates SK activity increases the parameter space where a bistable response of the TCS module to signals is possible, if SK is monofunctional, but decreases it when the SK is bifunctional. The presence of a third component that modulates RR activity decreases the parameter space where a bistable response of the TCS module to signals is possible.

  13. The fractional virial potential energy in two-component systems

    Directory of Open Access Journals (Sweden)

    Caimmi R.

    2008-01-01

    Full Text Available Two-component systems are conceived as macrogases, and the related equation of state is expressed using the virial theorem for subsystems, under the restriction of homeoidally striated density profiles. Explicit calculations are performed for a useful reference case and a few cases of astrophysical interest, both with and without truncation radius. Shallower density profiles are found to yield an equation of state, φ = φ(y, m, characterized (for assigned values of the fractional mass, m = Mj /Mi by the occurrence of two extremum points, a minimum and a maximum, as found in an earlier attempt. Steeper density profiles produce a similar equation of state, which implies that a special value of m is related to a critical curve where the above mentioned extremum points reduce to a single horizontal inflexion point, and curves below the critical one show no extremum points. The similarity of the isofractional mass curves to van der Waals' isothermal curves, suggests the possibility of a phase transition in a bell-shaped region of the (Oyφ plane, where the fractional truncation radius along a selected direction is y = Rj /Ri , and the fractional virial potential energy is φ = (Eji vir /(Eij vir . Further investigation is devoted to mass distributions described by Hernquist (1990 density profiles, for which an additional relation can be used to represent a sample of N = 16 elliptical galaxies (EGs on the (Oyφ plane. Even if the evolution of elliptical galaxies and their hosting dark matter (DM haloes, in the light of the model, has been characterized by equal fractional mass, m, and equal scaled truncation radius, or concentration, Ξu = Ru /r† , u = i, j, still it cannot be considered as strictly homologous, due to different values of fractional truncation radii, y, or fractional scaling radii, y† = r† /r† , deduced from sample objects.

  14. Brazilian two-component TLD albedo neutron individual monitoring system

    Energy Technology Data Exchange (ETDEWEB)

    Martins, M.M., E-mail: marcelo@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Mauricio, C.L.P., E-mail: claudia@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Fonseca, E.S. da, E-mail: evaldo@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD), Av. Salvador Allende, s/n, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Silva, A.X. da, E-mail: ademir@con.ufrj.b [Coordenacao dos Programas de Pos-Graduacao em Engenharia, COPPE/PEN Caixa Postal 68509, CEP: 21941-972, Rio de Janeiro, RJ (Brazil)

    2010-12-15

    Since 1983, Instituto de Radioprotecao e Dosimetria, Brazil, uses a TLD one-component albedo neutron monitor, which has a single different calibration factor specifically for each installation type. In order to improve its energy response, a two-component albedo monitor was developed, which measure the thermal neutron component besides the albedo one. The two-component monitor has been calibrated in reference neutron fields: thermal, five accelerator-produced monoenergetic beams (70, 144, 565, 1200 and 5000 keV) and five radionuclide sources ({sup 252}Cf, {sup 252}Cf(D{sub 2}O), {sup 241}Am-Be, {sup 241}Am-B and {sup 238}Pu-Be) at several distances. Since January 2008, mainly Brazilian workers who handle neutron sources at different distances and moderation, such as in well logging and calibration facilities are using it routinely.

  15. The Fractional Virial Potential Energy in Two-Component Systems

    Directory of Open Access Journals (Sweden)

    Caimmi, R.

    2008-12-01

    Full Text Available Two-component systems are conceived as macrogases, and the related equation of state is expressed using the virial theorem for subsystems, under the restriction of homeoidally striated density profiles. Explicit calculations are performed for a useful reference case and a few cases of astrophysical interest, both with and without truncation radius. Shallower density profiles are found to yield an equation of state, $phi=phi(y,m$, characterized (for assigned values of the fractional mass, $m=M_j/ M_i$ by the occurrence of two extremum points, a minimum and a maximum, as found in an earlier attempt. Steeper density profiles produce a similar equation of state, which implies that a special value of $m$ is related to a critical curve where the above mentioned extremum points reduce to a single horizontal inflexion point, and curves below the critical one show no extremum points. The similarity of the isofractional mass curves to van der Waals' isothermal curves, suggests the possibility of a phase transition in a bell-shaped region of the $({sf O}yphi$ plane, where the fractional truncation radius along a selected direction is $y=R_j/R_i$, and the fractional virial potential energy is $phi=(E_{ji}_mathrm{vir}/(E_{ij}_mathrm{vir}$. Further investigation is devoted to mass distributions described by Hernquist (1990 density profiles, for which an additional relation can be used to represent a sample of $N=16$ elliptical galaxies (EGs on the $({sf O}yphi$ plane. Even if the evolution of elliptical galaxies and their hosting dark matter (DM haloes, in the light of the model, has been characterized by equal fractional mass, $m$, and equal scaled truncation radius, or concentration, $Xi_u=R_u/r_u^dagger$, $u=i,j$, still it cannot be considered as strictly homologous, due to different values of fractional truncation radii, $y$, or fractional scaling radii, $y^dagger=r_j^dagger/r_i^dagger$, deduced from sample objects.

  16. A novel two-component system involved in secretion stress response in Streptomyces lividans.

    Directory of Open Access Journals (Sweden)

    Sonia Gullón

    Full Text Available BACKGROUND: Misfolded proteins accumulating outside the bacterial cytoplasmic membrane can interfere with the secretory machinery, hence the existence of quality factors to eliminate these misfolded proteins is of capital importance in bacteria that are efficient producers of secretory proteins. These bacteria normally use a specific two-component system to respond to the stress produced by the accumulation of the misfolded proteins, by activating the expression of HtrA-like proteases to specifically eliminate the incorrectly folded proteins. METHODOLOGY/PRINCIPAL FINDINGS: Overproduction of alpha-amylase in S. lividans causing secretion stress permitted the identification of a two-component system (SCO4156-SCO4155 that regulates three HtrA-like proteases which appear to be involved in secretion stress response. Mutants in each of the genes forming part of the two-genes operon that encodes the sensor and regulator protein components accumulated misfolded proteins outside the cell, strongly suggesting the involvement of this two-component system in the S. lividans secretion stress response. CONCLUSIONS/SIGNIFICANCE: To our knowledge this is the first time that a specific secretion stress response two-component system is found to control the expression of three HtrA-like protease genes in S. lividans, a bacterium that has been repeatedly used as a host for the synthesis of homologous and heterologous secretory proteins of industrial application.

  17. Cloning of a two-component signal transduction system of Xanthomonas campestris pv. phaseoli var. fuscans strain BXPF65

    DEFF Research Database (Denmark)

    Chan, JWYF; Maynard, Scott; Goodwin, PH

    1998-01-01

    A putative two-component signal transduction system was amplified and cloned from the plant pathogenic bacterium Xanthomonas campestris pv. phaseoli var. fuscans isolate BXPF65. The 620 bp amplified fragment was sequenced and analyzed with the BLAST Enhanced Alignment Utility (BEAUTY). BEAUTY...... that the putative histidine kinase has homology with conserved “transmitter” domains of sensor proteins in two-component signal transduction systems. RFLP analysis using the putative signal transduction system showed polymorphisms among the strains....

  18. Methods of producing epoxides from alkenes using a two-component catalyst system

    Science.gov (United States)

    Kung, Mayfair C.; Kung, Harold H.; Jiang, Jian

    2013-07-09

    Methods for the epoxidation of alkenes are provided. The methods include the steps of exposing the alkene to a two-component catalyst system in an aqueous solution in the presence of carbon monoxide and molecular oxygen under conditions in which the alkene is epoxidized. The two-component catalyst system comprises a first catalyst that generates peroxides or peroxy intermediates during oxidation of CO with molecular oxygen and a second catalyst that catalyzes the epoxidation of the alkene using the peroxides or peroxy intermediates. A catalyst system composed of particles of suspended gold and titanium silicalite is one example of a suitable two-component catalyst system.

  19. Regulation of virulence by a two-component system in group B streptococcus.

    Science.gov (United States)

    Jiang, Sheng-Mei; Cieslewicz, Michael J; Kasper, Dennis L; Wessels, Michael R

    2005-02-01

    Group B Streptococcus (GBS) is frequently carried in the gastrointestinal or genitourinary tract as a commensal organism, yet it has the potential to cause life-threatening infection in newborn infants, pregnant women, and individuals with chronic illness. Regulation of virulence factor expression may affect whether GBS behaves as an asymptomatic colonizer or an invasive pathogen, but little is known about how such factors are controlled in GBS. We now report the characterization of a GBS locus that encodes a two-component regulatory system similar to CsrRS (or CovRS) in Streptococcus pyogenes. Inactivation of csrR, encoding the putative response regulator, in two unrelated wild-type strains of GBS resulted in a marked increase in production of beta-hemolysin/cytolysin and a striking decrease in production of CAMP factor, an unrelated cytolytic toxin. Quantitative RNA hybridization experiments revealed that these two phenotypes were associated with a marked increase and decrease in expression of the corresponding genes, cylE and cfb, respectively. The CsrR mutant strains also displayed increased expression of scpB encoding C5a peptidase. Similar, but less marked, changes in gene expression were observed in CsrS (putative sensor component) mutants, evidence that CsrR and CsrS constitute a functional two-component system. Experimental infection studies in mice demonstrated reduced virulence of both CsrR and CsrS mutant strains relative to the wild type. Together, these results indicate that CsrRS regulates expression of multiple GBS virulence determinants and is likely to play an important role in GBS pathogenesis.

  20. Adaptation to Environmental Stimuli within the Host: Two-Component Signal Transduction Systems of Mycobacterium tuberculosis

    Science.gov (United States)

    Bretl, Daniel J.; Demetriadou, Chrystalla; Zahrt, Thomas C.

    2011-01-01

    Summary: Pathogenic microorganisms encounter a variety of environmental stresses following infection of their respective hosts. Mycobacterium tuberculosis, the etiological agent of tuberculosis, is an unusual bacterial pathogen in that it is able to establish lifelong infections in individuals within granulomatous lesions that are formed following a productive immune response. Adaptation to this highly dynamic environment is thought to be mediated primarily through transcriptional reprogramming initiated in response to recognition of stimuli, including low-oxygen tension, nutrient depletion, reactive oxygen and nitrogen species, altered pH, toxic lipid moieties, cell wall/cell membrane-perturbing agents, and other environmental cues. To survive continued exposure to these potentially adverse factors, M. tuberculosis encodes a variety of regulatory factors, including 11 complete two-component signal transduction systems (TCSSs) and several orphaned response regulators (RRs) and sensor kinases (SKs). This report reviews our current knowledge of the TCSSs present in M. tuberculosis. In particular, we discuss the biochemical and functional characteristics of individual RRs and SKs, the environmental stimuli regulating their activation, the regulons controlled by the various TCSSs, and the known or postulated role(s) of individual TCSSs in the context of M. tuberculosis physiology and/or pathogenesis. PMID:22126994

  1. Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12.

    Science.gov (United States)

    Oshima, Taku; Aiba, Hirofumi; Masuda, Yasushi; Kanaya, Shigehiko; Sugiura, Masahito; Wanner, Barry L; Mori, Hirotada; Mizuno, Takeshi

    2002-10-01

    We have systematically examined the mRNA profiles of 36 two-component deletion mutants, which include all two-component regulatory systems of Escherichia coli, under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria-Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two-component systems. A high correlation coefficient of expression profile was found between several two-component mutants. Together, these results support the view that a network of functional interactions, such as cross-regulation, exists between different two-component systems. The compiled data are avail-able at our website (http://ecoli.aist-nara.ac.jp/xp_analysis/ 2_components).

  2. Multiple Two-Component Systems of Streptococcus mutans Regulate Agmatine Deiminase Gene Expression and Stress Tolerance▿

    OpenAIRE

    Liu, Yaling; Burne, Robert A.

    2009-01-01

    Induction of the agmatine deiminase system (AgDS) of Streptococcus mutans requires agmatine and is optimal at low pH. We show here that the VicRK, ComDE, and CiaRH two-component systems influence AgDS gene expression in response to acidic and thermal stresses.

  3. Two-component air heating system. Final report. Zweikomponenten-Luftheizungs-System. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Radtke, W; Thiel, D

    1986-01-01

    The two-component heating system consists of a combination of air-based floor heating and direct air heating, with ventilation and extraction and heat recovery. The direct airflow consists exclusively of heated outside air, the amount corresponding to the building's external air intake requirement. The control system comprises a two-step sequential control of the air throughput of the direct air heating system and of the air distribution for the floor heating airflow. A special heating switch makes it possible to switch off the direct air heating system separately, and to select rapid warm-up. The way in which the new heating system works has been tested in a pilot set-up and proven by comprehensive measurements. In addition, a simulation model was produced which gave substantial confirmation of the measurements. (orig.) With 9 refs., 37 tabs., 63 figs.

  4. The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis

    DEFF Research Database (Denmark)

    Bisicchia, Paola; Noone, David; Lioliou, Efthimia

    2007-01-01

    Adaptation of bacteria to the prevailing environmental and nutritional conditions is often mediated by two-component signal transduction systems (TCS). The Bacillus subtilis YycFG TCS has attracted special attention as it is essential for viability and its regulon is poorly defined. Here we show...

  5. Two component system that regulates methanol and formaldehyde oxidation in Paracoccus denitrificans.

    NARCIS (Netherlands)

    Harms, N.; Reijnders, W.N.M.; Koning, S.; van Spanning, R.J.M.

    2001-01-01

    A chromosomal region encoding a two-component regulatory system, FlhRS, has been isolated from Paracoccus denitrificans. FlhRS-deficient mutants were unable to grow on methanol, methylamine, or choline as the carbon and energy source. Expression of the gene encoding glutathione-dependent

  6. Two-component system that regulates methanol and formaldehyde oxidation in Paracoccus denitrificans.

    NARCIS (Netherlands)

    Harms, N.; Reijnders, W.N.M.; Koning, S.; van Spanning, R.J.M.

    2001-01-01

    A chromosomal region encoding a two-component regulatory system, FlhRS, has been isolated from Paracoccus denitrificans. FlhRS-deficient mutants were unable to grow on methanol, methylamine, or choline as the carbon and energy source. Expression of the gene encoding glutathione-dependent

  7. Theoretical calculation of cryogenic distillation for two-component hydrogen isotope system

    International Nuclear Information System (INIS)

    Xia Xiulong; Luo Yangming; Wang Heyi; Fu Zhonghua; Liu Jun; Han Jun; Gu Mei

    2005-10-01

    Cryogenic distillation model for single column was built to simulating hydrogen isotope separation system. Three two-component system H 2 /HD, H 2 /HT and D 2 /DT was studied. Both temperature and concentration distribution was obtained and the results show a clear separation characteristics. H 2 /HT has the best separation performance while D 2 /DT was the most difficult to separate. (authors)

  8. Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity

    OpenAIRE

    Kawada-Matsuo, Miki; Komatsuzawa, Hitoshi

    2017-01-01

    Summary Approximately 100 trillion microorganisms exist in the oral cavity. For the commensal bacteria of the oral cavity, it is important to adapt to environmental stimuli, including human- or bacteria-derived antimicrobial agents. Recently, bacterial-specific signal transduction regulatory systems, called two-component systems (TCSs), which appear to be focused on sensing and adapting to the environment, were discovered. Streptococcus mutans is an oral commensal bacteria and is also known a...

  9. Multistability in an optomechanical system with a two-component Bose-Einstein condensate

    International Nuclear Information System (INIS)

    Dong Ying; Ye Jinwu; Pu Han

    2011-01-01

    We investigate a system consisting of a two-component Bose-Einstein condensate interacting dispersively with a Fabry-Perot optical cavity where the two components of the condensate are resonantly coupled to each other by another classical field. The key feature of this system is that the atomic motional degrees of freedom and the internal pseudospin degrees of freedom are coupled to the cavity field simultaneously, hence an effective spin-orbital coupling within the condensate is induced by the cavity. The interplay among the atomic center-of-mass motion, the atomic collective spin, and the cavity field leads to a strong nonlinearity, resulting in multistable behavior in both matter wave and light wave at the few-photon level.

  10. Two-Component Signal Transduction Systems That Regulate the Temporal and Spatial Expression of Myxococcus xanthus Sporulation Genes.

    Science.gov (United States)

    Sarwar, Zaara; Garza, Anthony G

    2016-02-01

    When starved for nutrients, Myxococcus xanthus produces a biofilm that contains a mat of rod-shaped cells, known as peripheral rods, and aerial structures called fruiting bodies, which house thousands of dormant and stress-resistant spherical spores. Because rod-shaped cells differentiate into spherical, stress-resistant spores and spore differentiation occurs only in nascent fruiting bodies, many genes and multiple levels of regulation are required. Over the past 2 decades, many regulators of the temporal and spatial expression of M. xanthus sporulation genes have been uncovered. Of these sporulation gene regulators, two-component signal transduction circuits, which typically contain a histidine kinase sensor protein and a transcriptional regulator known as response regulator, are among the best characterized. In this review, we discuss prototypical two-component systems (Nla6S/Nla6 and Nla28S/Nla28) that regulate an early, preaggregation phase of sporulation gene expression during fruiting body development. We also discuss orphan response regulators (ActB and FruA) that regulate a later phase of sporulation gene expression, which begins during the aggregation stage of fruiting body development. In addition, we summarize the research on a complex two-component system (Esp) that is important for the spatial regulation of sporulation. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  11. The Two-Component Virial Theorem and the Physical Properties of Stellar Systems.

    Science.gov (United States)

    Dantas; Ribeiro; Capelato; de Carvalho RR

    2000-01-01

    Motivated by present indirect evidence that galaxies are surrounded by dark matter halos, we investigate whether their physical properties can be described by a formulation of the virial theorem that explicitly takes into account the gravitational potential term representing the interaction of the dark halo with the baryonic or luminous component. Our analysis shows that the application of such a "two-component virial theorem" not only accounts for the scaling relations displayed by, in particular, elliptical galaxies, but also for the observed properties of all virialized stellar systems, ranging from globular clusters to galaxy clusters.

  12. Evaluation of solution stability for two-component polydisperse systems by small-angle scattering

    Science.gov (United States)

    Kryukova, A. E.; Konarev, P. V.; Volkov, V. V.

    2017-12-01

    The article is devoted to the modelling of small-angle scattering data using the program MIXTURE designed for the study of polydisperse multicomponent mixtures. In this work we present the results of solution stability studies for theoretical small-angle scattering data sets from two-component models. It was demonstrated that the addition of the noise to the data influences the stability range of the restored structural parameters. The recommendations for the optimal minimization schemes that permit to restore the volume size distributions for polydisperse systems are suggested.

  13. Phosphate sink containing two-component signaling systems as tunable threshold devices

    DEFF Research Database (Denmark)

    Amin, Munia; Kothamachu, Varun B; Feliu, Elisenda

    2014-01-01

    Synthetic biology aims to design de novo biological systems and reengineer existing ones. These efforts have mostly focused on transcriptional circuits, with reengineering of signaling circuits hampered by limited understanding of their systems dynamics and experimental challenges. Bacterial two......-component signaling systems offer a rich diversity of sensory systems that are built around a core phosphotransfer reaction between histidine kinases and their output response regulator proteins, and thus are a good target for reengineering through synthetic biology. Here, we explore the signal-response relationship...... rapid signal termination, whereby one of the RRs acts as a phosphate sink towards the other RR (i.e. the output RR), but also implements a sigmoidal signal-response relationship. We identify two mathematical conditions on system parameters that are necessary for sigmoidal signal-response relationships...

  14. A two-component system regulates hemin acquisition in Porphyromonas gingivalis.

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    Jodie C Scott

    Full Text Available Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidine kinase (PGN_0752, while the cognate response regulator (PGN_0753 remained intact. Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719. The data showed that the regulon of this signal transduction system contained genes that were involved in hemin acquisition, including gingipains, at least three transport systems, as well as being self-regulated. Direct regulation by the response regulator was confirmed by electrophoretic mobility shift assays. In addition, the system appears to be activated by hemin and the regulator acts as both an activator and repressor.

  15. Two-Component Signal Transduction System SaeRS Positively Regulates Staphylococcus epidermidis Glucose Metabolism

    Directory of Open Access Journals (Sweden)

    Qiang Lou

    2014-01-01

    Full Text Available Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS. Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.

  16. Functional assessment of EnvZ/OmpR two-component system in Shewanella oneidensis.

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    Jie Yuan

    Full Text Available EnvZ and OmpR constitute the bacterial two-component signal transduction system known to mediate osmotic stress response in a number of gram-negative bacteria. In an effort to understand the mechanism through which Shewanella oneidensis senses and responds to environmental osmolarity changes, structure of the ompR-envZ operon was determined with Northern blotting assay and roles of the EnvZ/OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR (qRT-PCR, and phenotype microarrays. Results from the mutational analysis and qRT-PCR suggested that the EnvZ/OmpR system contributed to osmotic stress response of S. oneidensis and very likely engaged a similar strategy employed by E. coli, which involved reciprocal regulation of two major porin coding genes. Additionally, the ompR-envZ system was also found related to cell motility. We further showed that the ompR-envZ dependent regulation of porin genes and motility resided almost completely on ompR and only partially on envZ, indicating additional mechanisms for OmpR phosphorylation. In contrast to E. coli lacking ompR-envZ, however, growth of S. oneidensis did not show a significant dependence on ompR-envZ even under osmotic stress. Further analysis with phenotype microarrays revealed that the S. oneidensis strains lacking a complete ompR-envZ system displayed hypersensitivities to a number of agents, especially in alkaline environment. Taken together, our results suggest that the function of the ompR-envZ system in S. oneidensis, although still connected with osmoregulation, has diverged considerably from that of E. coli. Additional mechanism must exist to support growth of S. oneidensis under osmotic stress.

  17. Lactoferricin B Inhibits the Phosphorylation of the Two-Component System Response Regulators BasR and CreB*

    Science.gov (United States)

    Ho, Yu-Hsuan; Sung, Tzu-Cheng; Chen, Chien-Sheng

    2012-01-01

    Natural antimicrobial peptides provide fundamental protection for multicellular organisms from microbes, such as Lactoferricin B (Lfcin B). Many studies have shown that Lfcin B penetrates the cell membrane and has intracellular activities. To elucidate the intracellular behavior of Lfcin B, we first used Escherichia coli K12 proteome chips to identify the intracellular targets of Lfcin B. The results showed that Lfcin B binds to two response regulators, BasR and CreB, of the two-component system. For further analysis, we conducted several in vitro and in vivo experiments and utilized bioinformatics methods. The electrophoretic mobility shift assays and kinase assays indicate that Lfcin B inhibits the phosphorylation of the response regulators (BasR and CreB) and their cognate sensor kinases (BasS and CreC). Antibacterial assays showed that Lfcin B reduced E. coli's tolerance to environmental stimuli, such as excessive ferric ions and minimal medium conditions. This is the first study to show that an antimicrobial peptide inhibits the growth of bacteria by influencing the phosphorylation of a two-component system directly. PMID:22138548

  18. Lactoferricin B inhibits the phosphorylation of the two-component system response regulators BasR and CreB.

    Science.gov (United States)

    Ho, Yu-Hsuan; Sung, Tzu-Cheng; Chen, Chien-Sheng

    2012-04-01

    Natural antimicrobial peptides provide fundamental protection for multicellular organisms from microbes, such as Lactoferricin B (Lfcin B). Many studies have shown that Lfcin B penetrates the cell membrane and has intracellular activities. To elucidate the intracellular behavior of Lfcin B, we first used Escherichia coli K12 proteome chips to identify the intracellular targets of Lfcin B. The results showed that Lfcin B binds to two response regulators, BasR and CreB, of the two-component system. For further analysis, we conducted several in vitro and in vivo experiments and utilized bioinformatics methods. The electrophoretic mobility shift assays and kinase assays indicate that Lfcin B inhibits the phosphorylation of the response regulators (BasR and CreB) and their cognate sensor kinases (BasS and CreC). Antibacterial assays showed that Lfcin B reduced E. coli's tolerance to environmental stimuli, such as excessive ferric ions and minimal medium conditions. This is the first study to show that an antimicrobial peptide inhibits the growth of bacteria by influencing the phosphorylation of a two-component system directly.

  19. Linearmycins Activate a Two-Component Signaling System Involved in Bacterial Competition and Biofilm Morphology

    Science.gov (United States)

    2017-01-01

    ABSTRACT Bacteria use two-component signaling systems to adapt and respond to their competitors and changing environments. For instance, competitor bacteria may produce antibiotics and other bioactive metabolites and sequester nutrients. To survive, some species of bacteria escape competition through antibiotic production, biofilm formation, or motility. Specialized metabolite production and biofilm formation are relatively well understood for bacterial species in isolation. How bacteria control these functions when competitors are present is not well studied. To address fundamental questions relating to the competitive mechanisms of different species, we have developed a model system using two species of soil bacteria, Bacillus subtilis and Streptomyces sp. strain Mg1. Using this model, we previously found that linearmycins produced by Streptomyces sp. strain Mg1 cause lysis of B. subtilis cells and degradation of colony matrix. We identified strains of B. subtilis with mutations in the two-component signaling system yfiJK operon that confer dual phenotypes of specific linearmycin resistance and biofilm morphology. We determined that expression of the ATP-binding cassette (ABC) transporter yfiLMN operon, particularly yfiM and yfiN, is necessary for biofilm morphology. Using transposon mutagenesis, we identified genes that are required for YfiLMN-mediated biofilm morphology, including several chaperones. Using transcriptional fusions, we found that YfiJ signaling is activated by linearmycins and other polyene metabolites. Finally, using a truncated YfiJ, we show that YfiJ requires its transmembrane domain to activate downstream signaling. Taken together, these results suggest coordinated dual antibiotic resistance and biofilm morphology by a single multifunctional ABC transporter promotes competitive fitness of B. subtilis. IMPORTANCE DNA sequencing approaches have revealed hitherto unexplored diversity of bacterial species in a wide variety of environments that

  20. [Regulation of sporulation by two-component system YvcPQ in Bacillus thuringiensis].

    Science.gov (United States)

    Fan, Qingyun; Zhang, Shumeng; Gong, Yujing; He, Jin

    2017-01-04

    To study the regulation of sporulation controlled by two-component system (TCS) YvcPQ. β-galactosidase experiment was used to verify the regulation of YvcP on kapD expression; bacterial one-hybrid assay, EMSA and RT-qPCR were applied to study the regulation of AbrB on yvcPQ expression; markerless gene deletion coupled with spore count was used to reveal the influence of yvcPQ and kapD expressions on sporulation. transcriptional regulator AbrB up-regulated the expression of yvcPQ; YvcP promoted the expression of kapD to inhibit sporulation. AbrB up-regulated the transcription of yvcPQ operon, then the increased YvcP strengthened the transcriptional acitivation of sporulation inhibitor gene kapD, and subsequently inhibited sporulation.

  1. TWO-COMPONENT SYSTEM: A MOLECULAR DIALOGUE BETWEEN RUMINAL BACTERIA AND FEED PARTICLES (FORAGE PLANTS

    Directory of Open Access Journals (Sweden)

    Monica Marcela Galicia Jimenez

    2017-12-01

    Full Text Available The ability to adapt rapidly to changes in the environment is one of the main characteristics of the bacterial cell. The rumen is a highly dynamic environment, and none of the changes are permanent due to the various microbial species found in the rumen. Signal transduction networks are information processing pathways that recognize various physical and chemical stimuli, amplification, signal processing, and trigger responses of the bacterial cell. The aim of the present review is to show the importance of these two component systems in rumen bacteria, because it is based on the knowledge of the principles governing the bacterial population communication, its main interactions and products of metabolism, we can approach the manipulation of Ruminal fermentation to improve animal health, productivity and food safety.

  2. A Unique Fungal Two-Component System Regulates Stress Responses, Drug Sensitivity, Sexual Development, and Virulence of Cryptococcus neoformans

    Science.gov (United States)

    Bahn, Yong-Sun; Kojima, Kaihei; Cox, Gary M.

    2006-01-01

    The stress-activated mitogen-activated protein kinase (MAPK) pathway is widely used by eukaryotic organisms as a central conduit via which cellular responses to the environment effect growth and differentiation. The basidiomycetous human fungal pathogen Cryptococcus neoformans uniquely uses the stress-activated Pbs2-Hog1 MAPK system to govern a plethora of cellular events, including stress responses, drug sensitivity, sexual reproduction, and virulence. Here, we characterized a fungal “two-component” system that controls these fundamental cellular functions via the Pbs2-Hog1 MAPK cascade. A typical response regulator, Ssk1, modulated all Hog1-dependent phenotypes by controlling Hog1 phosphorylation, indicating that Ssk1 is the major upstream signaling component of the Pbs2-Hog1 pathway. A second response regulator, Skn7, governs sensitivity to Na+ ions and the antifungal agent fludioxonil, negatively controls melanin production, and functions independently of Hog1 regulation. To control these response regulators, C. neoformans uses multiple sensor kinases, including two-component–like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. Our findings highlight unique adaptations of this global two-component MAPK signaling cascade in a ubiquitous human fungal pathogen. PMID:16672377

  3. Development and characterization of two-component albedo based neutron individual monitoring system using thermoluminescent detectors

    International Nuclear Information System (INIS)

    Martins, Marcelo Marques

    2008-01-01

    A TLD-albedo based two-component neutron individual monitoring system was developed and characterized in this work. The monitor consists of a black plastic holder, an incident neutron boron loaded shield, a moderator polyethylene body (to increase its response), two pairs of TLD-600 and TLD-700 (one pair to each component) and an adjustable belt. This monitoring system was calibrated in thermal neutron fields and in 70 keV, 144 keV, 565 keV, 1.2 MeV and 5 MeV monoenergetic neutron fields. In addition, it was calibrated in 252C f(D 2 O), 252 Cf, 241 Am-B, 241 Am-Be and 238 Pu-Be source fields. For the latter, the lower detection levels are, respectively, 0.009 mSv, 0.06 mSv, 0.12 mSv, 0.09 mSv and 0.08 mSv. The participation in an international intercomparison sponsored by IAEA with simulated workplace fields validated the system. The monitoring system was successfully characterized in the ISO 21909 standard and in an IRD - the Brazilian Institute for Radioprotection and Dosimetry - technical regulation draft. Nowadays, the neutron individual system is in use by IRD for whole body individual monitoring of five institutions, which comprehend several activities. (author)

  4. Counterbalancing Regulation in Response Memory of a Positively Autoregulated Two-Component System.

    Science.gov (United States)

    Gao, Rong; Godfrey, Katherine A; Sufian, Mahir A; Stock, Ann M

    2017-09-15

    Fluctuations in nutrient availability often result in recurrent exposures to the same stimulus conditions. The ability to memorize the past event and use the "memory" to make adjustments to current behaviors can lead to a more efficient adaptation to the recurring stimulus. A short-term phenotypic memory can be conferred via carryover of the response proteins to facilitate the recurrent response, but the additional accumulation of response proteins can lead to a deviation from response homeostasis. We used the Escherichia coli PhoB/PhoR two-component system (TCS) as a model system to study how cells cope with the recurrence of environmental phosphate (Pi) starvation conditions. We discovered that "memory" of prior Pi starvation can exert distinct effects through two regulatory pathways, the TCS signaling pathway and the stress response pathway. Although carryover of TCS proteins can lead to higher initial levels of transcription factor PhoB and a faster initial response in prestarved cells than in cells not starved, the response enhancement can be overcome by an earlier and greater repression of promoter activity in prestarved cells due to the memory of the stress response. The repression counterbalances the carryover of the response proteins, leading to a homeostatic response whether or not cells are prestimulated. A computational model based on sigma factor competition was developed to understand the memory of stress response and to predict the homeostasis of other PhoB-regulated response proteins. Our insight into the history-dependent PhoBR response may provide a general understanding of how TCSs respond to recurring stimuli and adapt to fluctuating environmental conditions. IMPORTANCE Bacterial cells in their natural environments experience scenarios that are far more complex than are typically replicated in laboratory experiments. The architectures of signaling systems and the integration of multiple adaptive pathways have evolved to deal with such complexity

  5. Counterbalancing Regulation in Response Memory of a Positively Autoregulated Two-Component System

    Science.gov (United States)

    Gao, Rong; Godfrey, Katherine A.; Sufian, Mahir A.

    2017-01-01

    ABSTRACT Fluctuations in nutrient availability often result in recurrent exposures to the same stimulus conditions. The ability to memorize the past event and use the “memory” to make adjustments to current behaviors can lead to a more efficient adaptation to the recurring stimulus. A short-term phenotypic memory can be conferred via carryover of the response proteins to facilitate the recurrent response, but the additional accumulation of response proteins can lead to a deviation from response homeostasis. We used the Escherichia coli PhoB/PhoR two-component system (TCS) as a model system to study how cells cope with the recurrence of environmental phosphate (Pi) starvation conditions. We discovered that “memory” of prior Pi starvation can exert distinct effects through two regulatory pathways, the TCS signaling pathway and the stress response pathway. Although carryover of TCS proteins can lead to higher initial levels of transcription factor PhoB and a faster initial response in prestarved cells than in cells not starved, the response enhancement can be overcome by an earlier and greater repression of promoter activity in prestarved cells due to the memory of the stress response. The repression counterbalances the carryover of the response proteins, leading to a homeostatic response whether or not cells are prestimulated. A computational model based on sigma factor competition was developed to understand the memory of stress response and to predict the homeostasis of other PhoB-regulated response proteins. Our insight into the history-dependent PhoBR response may provide a general understanding of how TCSs respond to recurring stimuli and adapt to fluctuating environmental conditions. IMPORTANCE Bacterial cells in their natural environments experience scenarios that are far more complex than are typically replicated in laboratory experiments. The architectures of signaling systems and the integration of multiple adaptive pathways have evolved to deal

  6. A two component system is involved in acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus.

    Science.gov (United States)

    Cui, Yanhua; Liu, Wei; Qu, Xiaojun; Chen, Zhangting; Zhang, Xu; Liu, Tong; Zhang, Lanwei

    2012-05-20

    The Gram-positive bacterium Lactobacillus delbrueckii subsp. bulgaricus is of vital importance to the food industry, especially to the dairy industry. Two component systems (TCSs) are one of the most important mechanisms for environmental sensing and signal transduction in the majority of Gram-positive and Gram-negative bacteria. A typical TCS consists of a histidine protein kinase (HPK) and a cytoplasmic response regulator (RR). To investigate the functions of TCSs during acid adaptation in L. bulgaricus, we used quantitative PCR to reveal how TCSs expression changes during acid adaptation. Two TCSs (JN675228/JN675229 and JN675230/JN675231) and two HPKs (JN675236 and JN675240) were induced during acid adaptation. These TCSs were speculated to be related with the acid adaptation ability of L. bulgaricus. The mutants of JN675228/JN675229 were constructed in order to investigate the functions of JN675228/JN675229. The mutants showed reduced acid adaptation compared to that of wild type, and the complemented strains were similar to the wild-type strain. These observations suggested that JN675228 and JN675229 were involved in acid adaptation in L. bulgaricus. The interaction between JN675228 and JN675229 was identified by means of yeast two-hybrid system. The results indicated there is interaction between JN675228 and JN675229. Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.

  7. The Evolution of Two-Component Systems in Bacteria RevealsDifferent Strategies for Niche Adaptation

    Energy Technology Data Exchange (ETDEWEB)

    Alm, Eric; Huang, Katherine; Arkin, Adam

    2006-09-13

    Two-component systems including histidine protein kinasesrepresent the primary signal transduction paradigm in prokaryoticorganisms. To understand how these systems adapt to allow organisms todetect niche-specific signals, we analyzed the phylogenetic distributionof nearly 5000 histidine protein kinases from 207 sequenced prokaryoticgenomes. We found that many genomes carry a large repertoire of recentlyevolved signaling genes, which may reflect selective pressure to adapt tonew environmental conditions. Both lineage-specific gene family expansionand horizontal gene transfer play major roles in the introduction of newhistidine kinases into genomes; however, there are differences in howthese two evolutionary forces act. Genes imported via horizontal transferare more likely to retain their original functionality as inferred from asimilar complement of signaling domains, while gene family expansionaccompanied by domain shuffling appears to be a major source of novelgenetic diversity. Family expansion is the dominantsource of newhistidine kinase genes in the genomes most enriched in signalingproteins, and detailed analysis reveals that divergence in domainstructure and changes in expression patterns are hallmarks of recentexpansions. Finally, while these two modes of gene acquisition arewidespread across bacterial taxa, there are clear species-specificpreferences for which mode is used.

  8. A hybrid two-component system protein from Azospirillum brasilense Sp7 was involved in chemotaxis.

    Science.gov (United States)

    Cui, Yanhua; Tu, Ran; Wu, Lixian; Hong, Yuanyuan; Chen, Sanfeng

    2011-09-20

    We here report the sequence and functional analysis of org35 of Azospirillum brasilense Sp7, which was originally identified to be able to interact with NifA in yeast-two-hybrid system. The org35 encodes a hybrid two-component system protein, including N-terminal PAS domains, a histidine kinase (HPK) domain and a response regulator (RR) domain in C-terminal. To determine the function of the Org35, a deletion-insertion mutant in PAS domain [named Sp7353] and a complemental strain Sp7353C were constructed. The mutant had reduced chemotaxis ability compared to that of wild-type, and the complemental strain was similar to the wild-type strain. These data suggested that the A. brasilense org35 played a key role in chemotaxis. Variants containing different domains of the org35 were expressed, and the functions of these domains were studied in vitro. Phosphorylation assays in vitro demonstrated that the HPK domain of Org35 possessed the autokinase activity and that the phosphorylated HPK was able to transfer phosphate groups to the RR domain. The result indicated Org35 was a phosphorylation-communicating protein. Copyright © 2010 Elsevier GmbH. All rights reserved.

  9. Thermodynamics and kinetics of interstitial diffusion in a two-component system

    International Nuclear Information System (INIS)

    McKee, R.A.

    1980-01-01

    Diffusion theory is developed for a two-component system in which only the interstitial element is mobile. A thermodynamic formalism is used in direct parallel with a kinetic theory to construct a mechanism-independent relationship between tracer- and chemical-diffusion coefficients. It is found that D/sup I/=(D-italic*/f)(1+partiallnγ/partiallnC). D/sup I/ is the intrinsic- or chemical-diffusion coefficient for the interstitial, D* is the tracer-diffusion coefficient, f is the correlation factor, and γ is the activity coefficient. This expression accounts for site exclusion, correlation, and drift effects that occur as the interstitial content changes. Generalized phenomenological coefficients that are determined in this analysis can be used for standard representations of diffusion in electric fields and temperature gradients. Moreover, the forms that the phenomenological coefficients take for the interstitial system are the same as those previously derived for vacancy diffusion. A test of this predicted relationship between tracer- and chemical-diffusion coefficients is developed using a comparison between theory and experiment for carbon diffusion in fcc iron

  10. Transcriptome analysis of the Brucella abortus BvrR/BvrS two-component regulatory system.

    Directory of Open Access Journals (Sweden)

    Cristina Viadas

    Full Text Available BACKGROUND: The two-component BvrR/BvrS system is essential for Brucella abortus virulence. It was shown previously that its dysfunction alters the expression of some major outer membrane proteins and the pattern of lipid A acylation. To determine the genes regulated by BvrR/BvrS, we performed a whole-genome microarray analysis using B. abortus RNA obtained from wild type and bvrR mutant cells grown in the same conditions. METHODOLOGY/PRINCIPAL FINDINGS: A total of 127 differentially expressed genes were found: 83 were over expressed and 44 were less expressed in the bvrR mutant. Two operons, the phosphotransferase system and the maltose transport system, were down-regulated. Several genes involved in cell envelope or outer membrane biogenesis were differentially expressed: genes for outer membrane proteins (omp25a, omp25d, lipoproteins, LPS and fatty acid biosynthesis, stress response proteins, chaperones, flagellar genes, and twelve genes encoding ABC transport systems. Ten genes related with carbon metabolism (pckA and fumB among others were up-regulated in the bvrR mutant, and denitrification genes (nirK, norC and nosZ were also regulated. Notably, seven transcriptional regulators were affected, including VjbR, ExoR and OmpR that were less expressed in the bvrR mutant. Finally, the expression of eleven genes which have been previously related with Brucella virulence was also altered. CONCLUSIONS/SIGNIFICANCE: All these data corroborate the impact of BvrR/BvrS on cell envelope modulation, confirm that this system controls the carbon and nitrogen metabolism, and suggest a cross-talk among some regulators to adjust the Brucella physiology to the shift expected to occur during the transit from the extracellular to the intracellular niche.

  11. A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Alvarez-Martin, Pablo; O'Connell Motherway, Mary; Turroni, Francesca; Foroni, Elena; Ventura, Marco; van Sinderen, Douwe

    2012-10-01

    This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), which are predicted to encode a hypothetical membrane-associated protein and a serpin-like protein, respectively. The response regulator SerR was shown to bind to the promoter region of ser(2003), and the probable recognition sequence of SerR was determined by a combinatorial approach of in vitro site-directed mutagenesis coupled to transcriptional fusion and electrophoretic mobility shift assays (EMSAs). The importance of the serRK 2CRS in the response of B. breve to protease-mediated induction was confirmed by generating a B. breve serR insertion mutant, which was shown to exhibit altered ser(2003) transcriptional induction patterns compared to the parent strain, UCC2003. Interestingly, the analysis of a B. breve serU mutant revealed that the SerRK signaling pathway appears to include a SerU-dependent autoregulatory loop.

  12. Evolutionary tuning of protein expression levels of a positively autoregulated two-component system.

    Directory of Open Access Journals (Sweden)

    Rong Gao

    2013-10-01

    Full Text Available Cellular adaptation relies on the development of proper regulatory schemes for accurate control of gene expression levels in response to environmental cues. Over- or under-expression can lead to diminished cell fitness due to increased costs or insufficient benefits. Positive autoregulation is a common regulatory scheme that controls protein expression levels and gives rise to essential features in diverse signaling systems, yet its roles in cell fitness are less understood. It remains largely unknown how much protein expression is 'appropriate' for optimal cell fitness under specific extracellular conditions and how the dynamic environment shapes the regulatory scheme to reach appropriate expression levels. Here, we investigate the correlation of cell fitness and output response with protein expression levels of the E. coli PhoB/PhoR two-component system (TCS. In response to phosphate (Pi-depletion, the PhoB/PhoR system activates genes involved in phosphorus assimilation as well as genes encoding themselves, similarly to many other positively autoregulated TCSs. We developed a bacteria competition assay in continuous cultures and discovered that different Pi conditions have conflicting requirements of protein expression levels for optimal cell fitness. Pi-replete conditions favored cells with low levels of PhoB/PhoR while Pi-deplete conditions selected for cells with high levels of PhoB/PhoR. These two levels matched PhoB/PhoR concentrations achieved via positive autoregulation in wild-type cells under Pi-replete and -deplete conditions, respectively. The fitness optimum correlates with the wild-type expression level, above which the phosphorylation output saturates, thus further increase in expression presumably provides no additional benefits. Laboratory evolution experiments further indicate that cells with non-ideal protein levels can evolve toward the optimal levels with diverse mutational strategies. Our results suggest that the natural

  13. Characterization of an archaeal two-component system that regulates methanogenesis in Methanosaeta harundinacea.

    Directory of Open Access Journals (Sweden)

    Jie Li

    Full Text Available Two-component signal transduction systems (TCSs are a major mechanism used by bacteria in response to environmental changes. Although many sequenced archaeal genomes encode TCSs, they remain poorly understood. Previously, we reported that a methanogenic archaeon, Methanosaeta harundinacea, encodes FilI, which synthesizes carboxyl-acyl homoserine lactones, to regulate transitions of cellular morphology and carbon metabolic fluxes. Here, we report that filI, the cotranscribed filR2, and the adjacent filR1 constitute an archaeal TCS. FilI possesses a cytoplasmic kinase domain (histidine kinase A and histidine kinase-like ATPase and its cognate response regulator. FilR1 carries a receiver (REC domain coupled with an ArsR-related domain with potential DNA-binding ability, while FilR2 carries only a REC domain. In a phosphorelay assay, FilI was autophosphorylated and specifically transferred the phosphoryl group to FilR1 and FilR2, confirming that the three formed a cognate TCS. Through chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR using an anti-FilR1 antibody, FilR1 was shown to form in vivo associations with its own promoter and the promoter of the filI-filR2 operon, demonstrating a regulatory pattern common among TCSs. ChIP-qPCR also detected FilR1 associations with key genes involved in acetoclastic methanogenesis, acs4 and acs1. Electrophoretic mobility shift assays confirmed the in vitro tight binding of FilR1 to its own promoter and those of filI-filR2, acs4, and mtrABC. This also proves the DNA-binding ability of the ArsR-related domain, which is found primarily in Archaea. The archaeal promoters of acs4, filI, acs1, and mtrABC also initiated FilR1-modulated expression in an Escherichia coli lux reporter system, suggesting that FilR1 can up-regulate both archaeal and bacterial transcription. In conclusion, this work identifies an archaeal FilI/FilRs TCS that regulates the methanogenesis of M. harundinacea.

  14. Morphology-tunable and photoresponsive properties in a self-assembled two-component gel system.

    Science.gov (United States)

    Zhou, Yifeng; Xu, Miao; Yi, Tao; Xiao, Shuzhang; Zhou, Zhiguo; Li, Fuyou; Huang, Chunhui

    2007-01-02

    Photoresponsive C3-symmetrical trisurea self-assembling building blocks containing three azobenzene groups (LC10 and LC4) at the rim were designed and synthesized. By introducing a trisamide gelator (G18), which can self-aggregate through hydrogen bonds of acylamino moieties to form a fibrous network, the mixture of LC10 (or LC4) and G18 forms an organogel with coral-like supramolecular structure from 1,4-dioxane. The cooperation of hydrogen bonding and the hydrophobic diversity between these components are the main contributions to the specific superstructure. The two-component gel exhibits reversible photoisomerization from trans to cis transition without breakage of the gel state.

  15. Bacillus subtilis Two-Component System Sensory Kinase DegS Is Regulated by Serine Phosphorylation in Its Input Domain

    DEFF Research Database (Denmark)

    Jers, Carsten; Kobir, Ahasanul; Søndergaard, Elsebeth Oline

    2011-01-01

    Bacillus subtilis two-component system DegS/U is well known for the complexity of its regulation. The cytosolic sensory kinase DegS does not receive a single predominant input signal like most two-component kinases, instead it integrates a wide array of metabolic inputs that modulate its activity......S phosphorylation can be carried out by at least two B. subtilis Hanks-type kinases in vitro, and this stimulates the phosphate transfer towards DegU. The consequences of this process were studied in vivo, using phosphomimetic (Ser76Asp) and non-phosphorylatable (Ser76Ala) mutants of DegS. In a number...

  16. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    NARCIS (Netherlands)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

    2008-01-01

    Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

  17. A Conserved Two-Component Signal Transduction System Controls the Response to Phosphate Starvation in Bifidobacterium breve UCC2003.

    NARCIS (Netherlands)

    Alvarez-Martin, P.; Fernandez, M.; O'Connell-Motherway, M.; O'Connell, K.J.; Sauvageot, N.; Fitzgerald, G.F.; Macsharry, J.; Zomer, A.L.; Sinderen, D. van

    2012-01-01

    This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P(i)) starvation in Bifidobacterium breve UCC2003. The response regulator PhoP was shown to bind to the promoter region of

  18. Evolutionary history of the OmpR/IIIA family of signal transduction two component systems in Lactobacillaceae and Leuconostocaceae

    Directory of Open Access Journals (Sweden)

    González-Candelas Fernando

    2011-02-01

    Full Text Available Abstract Background Two component systems (TCS are signal transduction pathways which typically consist of a sensor histidine kinase (HK and a response regulator (RR. In this study, we have analyzed the evolution of TCS of the OmpR/IIIA family in Lactobacillaceae and Leuconostocaceae, two families belonging to the group of lactic acid bacteria (LAB. LAB colonize nutrient-rich environments such as foodstuffs, plant materials and the gastrointestinal tract of animals thus driving the study of this group of both basic and applied interest. Results The genomes of 19 strains belonging to 16 different species have been analyzed. The number of TCS encoded by the strains considered in this study varied between 4 in Lactobacillus helveticus and 17 in Lactobacillus casei. The OmpR/IIIA family was the most prevalent in Lactobacillaceae accounting for 71% of the TCS present in this group. The phylogenetic analysis shows that no new TCS of this family has recently evolved in these Lactobacillaceae by either lineage-specific gene expansion or domain shuffling. Furthermore, no clear evidence of non-orthologous replacements of either RR or HK partners has been obtained, thus indicating that coevolution of cognate RR and HKs has been prevalent in Lactobacillaceae. Conclusions The results obtained suggest that vertical inheritance of TCS present in the last common ancestor and lineage-specific gene losses appear as the main evolutionary forces involved in their evolution in Lactobacillaceae, although some HGT events cannot be ruled out. This would agree with the genomic analyses of Lactobacillales which show that gene losses have been a major trend in the evolution of this group.

  19. Study of the catalytic selectivity of an aqueous two-component polyurethane system by ftir spectroscopy

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    Stamenković Jakov V.

    2003-01-01

    Full Text Available The difficulty in formulating a two component waterborne polyurethane, is the isocyanate-water side reaction, which can lead to gassing/foaming, loss of isocyanate functionality, low gloss and a reduced pot life. To compensate for this side reaction, these formulations usually contain a large excess of isocyanate. Tin compounds, especially dibutyltin dilaurate, are widely used in coatings as catalysts for the isocyanate/hydroxyl reaction. Because of the high aquatic toxicity of some organotin compounds, there has been an attempt to ban organotin compounds from all coating applications. As a general rule, organotin catalysts are not selective, they catalyze the reaction of isocyanates with both hydroxyl groups and water and also catalyze the hydrolysis of ester groups. One novel approach to control the water side reaction is the use of catalysts which selectively catalyze the isocyanate-polyol reaction and not the isocyanate-water reaction. The selectivity of a variety of metal catalysts (metal octoates, metal acetylacetonates and mangan chelates with mixed ligands to catalyze the preferred reaction was measured using the FTIR method.

  20. Molecular evolution of the two-component system BvgAS involved in virulence regulation in Bordetella.

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    Julien Herrou

    Full Text Available The whooping cough agent Bordetella pertussis is closely related to Bordetella bronchiseptica, which is responsible for chronic respiratory infections in various mammals and is occasionally found in humans, and to Bordetella parapertussis, one lineage of which causes mild whooping cough in humans and the other ovine respiratory infections. All three species produce similar sets of virulence factors that are co-regulated by the two-component system BvgAS. We characterized the molecular diversity of BvgAS in Bordetella by sequencing the two genes from a large number of diverse isolates. The response regulator BvgA is virtually invariant, indicating strong functional constraints. In contrast, the multi-domain sensor kinase BvgS has evolved into two different types. The pertussis type is found in B. pertussis and in a lineage of essentially human-associated B. bronchiseptica, while the bronchiseptica type is associated with the majority of B. bronchiseptica and both ovine and human B. parapertussis. BvgS is monomorphic in B. pertussis, suggesting optimal adaptation or a recent population bottleneck. The degree of diversity of the bronchiseptica type BvgS is markedly different between domains, indicating distinct evolutionary pressures. Thus, absolute conservation of the putative solute-binding cavities of the two periplasmic Venus Fly Trap (VFT domains suggests that common signals are perceived in all three species, while the external surfaces of these domains vary more extensively. Co-evolution of the surfaces of the two VFT domains in each type and domain swapping experiments indicate that signal transduction in the periplasmic region may be type-specific. The two distinct evolutionary solutions for BvgS confirm that B. pertussis has emerged from a specific B. bronchiseptica lineage. The invariant regions of BvgS point to essential parts for its molecular mechanism, while the variable regions may indicate adaptations to different lifestyles. The

  1. Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches

    DEFF Research Database (Denmark)

    Tucker, Paul; Nowak, Elzbieta; Morth, Jens Preben

    2007-01-01

    Mycobacterium tuberculosis contains few two‐component systems compared to many other bacteria, possibly because it has more serine/threonine signaling pathways. Even so, these two‐component systems appear to play an important role in early intracellular survival of the pathogen as well as in aspe...

  2. The oligomeric assembly of the novel haem-degrading protein HbpS is essential for interaction with its cognate two-component sensor kinase

    NARCIS (Netherlands)

    Ortiz de Orué Lucana, Darío; Bogel, Gabriele; Zou, Peijian; Groves, Matthew R

    2009-01-01

    HbpS, a novel protein of previously unknown function from Streptomyces reticuli, is up-regulated in response to haemin- and peroxide-based oxidative stress and interacts with the SenS/SenR two-component signal transduction system. In this study, we report the high-resolution crystal structures (2.2

  3. Effects of non-uniform temperature gradients on surface tension driven two component magneto convection in a porous- fluid system

    Science.gov (United States)

    Manjunatha, N.; Sumithra, R.

    2018-04-01

    The problem of surface tension driven two component magnetoconvection is investigated in a Porous-Fluid system, consisting of anincompressible two component electrically conducting fluid saturatedporous layer above which lies a layer of the same fluid in the presence of a uniform vertical magnetic field. The lower boundary of the porous layeris rigid and the upper boundary of the fluid layer is free with surfacetension effects depending on both temperature and concentration, boththese boundaries are insulating to heat and mass. At the interface thevelocity, shear and normal stress, heat and heat flux, mass and mass fluxare assumed to be continuous suitable for Darcy-Brinkman model. Theeigenvalue problem is solved in linear, parabolic and inverted parabolictemperature profiles and the corresponding Thermal Marangoni Numberis obtained for different important physical parameters.

  4. On various integrable discretizations of a general two-component Volterra system

    International Nuclear Information System (INIS)

    Babalic, Corina N; Carstea, A S

    2013-01-01

    We present two integrable discretizations of a general differential–difference bicomponent Volterra system. The results are obtained by discretizing directly the corresponding Hirota bilinear equations in two different ways. Multisoliton solutions are presented together with a new discrete form of Lotka–Volterra equation obtained by an alternative bilinearization. (paper)

  5. Lignin Structure and Aggregation Behavior in a Two-Component Ionic Liquid Solvent System

    Directory of Open Access Journals (Sweden)

    Susanne Bylin

    2014-08-01

    Full Text Available Ionic liquids are of potential interest in the processing of lignocellulosic biomass. In this study, the ionic liquid co-solvent system of 1-methylimidazole (MIM and 1-ethyl-3-methyl-imidazolium acetate (EMIMAc was used to solvate LignoBoost lignin fractionated from black liquor obtained from a kraft paper mill. Lignin ethanol-precipitated (LEP and ethanol-soluble (LES fractions were characterized via gel permeation chromatography (GPC and 13C- and 31P-nuclear magnetic resonance spectroscopy (NMR to determine structural characteristics and their relationship to polymer solubility in the system. Polymer integrity and solubility were optimal at ~20% lignin loading (w/w. Results showed that LEPs were generally of higher apparent molecular weight (Mw and enriched with condensed/aliphatic ether linkages and aliphatic hydroxyls. The LESs had a lower apparent Mw and were enriched with carboxylic and phenolic groups. This newly gained knowledge on lignin fractionation and aggregation in the present solvent system provides future opportunities for tuning fractionation/extraction to suit a specific biomass-derived product, e.g., carbon fibers.

  6. Accelerator system for producing two-component beams for studies of interactive surface effects

    International Nuclear Information System (INIS)

    Kaminsky, M.; Das, S.K.; Ekern, R.; Hess, D.C.

    1977-01-01

    For studies of interactive surface effects caused by the simultaneous bombardment of targets by both chemically active and inactive ion species (e.g., D + and He + , respectively) a two beam component accelerator facility was placed in operation. One component, consisting of light ions (e.g., H, D, He) is accelerated by a 2-MV Van de Graaff accelerator which provides a mass analyzed and focussed beam for the energy range from approximately 100-keV to 2-MeV (for singly charged ions). The other component is a beam of light ions in the energy range from approximately 10-keV to 100-keV. This is furnished by a 100-kV dc accelerator system which provides a mass analyzed focussed beam. This beam is guided into the beam line of the Van de Graaff accelerator electrostatically, and with the aid of beam steerers it is made to be co-axial with the Van de Graaff generated beam. The angle of incidence becomes hereby a free parameter for the interaction of the mixed beams with a surface. For each beam component, current densities of 650 μA cm -2 on target can readily be obtained. In order to reduce carbon contamination of the irradiated targets significantly, stainless steel beam lines have been used together with a combination of turbomolecular pumps and ion-sublimation pumps.A total pressure of 2 to 3 x 10 -8 torr in the beam lines and of 2 x 10 -9 torr in the target chamber can be obtained readily. Experimental results on the surface damage of Ni bombarded simultaneously with He + and D + ions are presented. The importance of such studies of interactive surface effects for the controlled thermonuclear fusion program are discussed

  7. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

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    Nguyen-the Christophe

    2008-10-01

    Full Text Available Abstract Background Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. Results Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. Conclusion The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

  8. The two-component system VicRK regulates functions associated with Streptococcus mutans resistance to complement immunity.

    Science.gov (United States)

    Alves, Livia A; Harth-Chu, Erika N; Palma, Thais H; Stipp, Rafael N; Mariano, Flávia S; Höfling, José F; Abranches, Jacqueline; Mattos-Graner, Renata O

    2017-10-01

    Streptococcus mutans, a dental caries pathogen, can promote systemic infections upon reaching the bloodstream. The two-component system (TCS) VicRK Sm of S. mutans regulates the synthesis of and interaction with sucrose-derived exopolysaccharides (EPS), processes associated with oral and systemic virulence. In this study, we investigated the mechanisms by which VicRK Sm affects S. mutans susceptibility to blood-mediated immunity. Compared with parent strain UA159, the vicK Sm isogenic mutant (UAvic) showed reduced susceptibility to deposition of C3b of complement, low binding to serum immunoglobulin G (IgG), and low frequency of C3b/IgG-mediated opsonophagocytosis by polymorphonuclear cells in a sucrose-independent way (Pmutans employs mechanisms of complement evasion through peptidases, which are controlled by VicRK Sm. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  9. VfrB Is a Key Activator of the Staphylococcus aureus SaeRS Two-Component System.

    Science.gov (United States)

    Krute, Christina N; Rice, Kelly C; Bose, Jeffrey L

    2017-03-01

    In previous studies, we identified the fatty acid kinase virulence factor regulator B (VfrB) as a potent regulator of α-hemolysin and other virulence factors in Staphylococcus aureus In this study, we demonstrated that VfrB is a positive activator of the SaeRS two-component regulatory system. Analysis of vfrB , saeR , and saeS mutant strains revealed that VfrB functions in the same pathway as SaeRS. At the transcriptional level, the promoter activities of SaeRS class I ( coa ) and class II ( hla ) target genes were downregulated during the exponential growth phase in the vfrB mutant, compared to the wild-type strain. In addition, saePQRS expression was decreased in the vfrB mutant strain, demonstrating a need for this protein in the autoregulation of SaeRS. The requirement for VfrB-mediated activation was circumvented when SaeS was constitutively active due to an SaeS (L18P) substitution. Furthermore, activation of SaeS via human neutrophil peptide 1 (HNP-1) overcame the dependence on VfrB for transcription from class I Sae promoters. Consistent with the role of VfrB in fatty acid metabolism, hla expression was decreased in the vfrB mutant with the addition of exogenous myristic acid. Lastly, we determined that aspartic acid residues D38 and D40, which are predicted to be key to VfrB enzymatic activity, were required for VfrB-mediated α-hemolysin production. Collectively, this study implicates VfrB as a novel accessory protein needed for the activation of SaeRS in S. aureus IMPORTANCE The SaeRS two-component system is a key regulator of virulence determinant production in Staphylococcus aureus Although the regulon of this two-component system is well characterized, the activation mechanisms, including the specific signaling molecules, remain elusive. Elucidating the complex regulatory circuit of SaeRS regulation is important for understanding how the system contributes to disease causation by this pathogen. To this end, we have identified the fatty acid kinase

  10. Genome-wide DNA binding pattern of two-component system response regulator RhpR in Pseudomonas syringae

    Directory of Open Access Journals (Sweden)

    Tianhong Zhou

    2015-06-01

    Full Text Available Although Pseudomonas syringae uses the two-component system RhpRS to modulate the expression of type III secretion system (T3SS genes and pathogenicity, the molecular mechanisms and the regulon of RhpRS have yet to be fully demonstrated. We have performed a genome-wide analysis of RhpR binding to DNA prepared from P. syringae pv. phaseolicola in order to identify candidate direct targets of RhpR-mediated transcriptional regulation, as described in our recent article [1]. The data are available from NCBI Gene Expression Omnibus (GEO with the accession number GSE58533. Here we describe the detailed methods and data analyses of our RhpR ChIP-seq dataset.

  11. Characterization of virulence factor regulation by SrrAB, a two-component system in Staphylococcus aureus.

    Science.gov (United States)

    Pragman, Alexa A; Yarwood, Jeremy M; Tripp, Timothy J; Schlievert, Patrick M

    2004-04-01

    Workers in our laboratory have previously identified the staphylococcal respiratory response AB (SrrAB), a Staphylococcus aureus two-component system that acts in the global regulation of virulence factors. This system down-regulates production of agr RNAIII, protein A, and toxic shock syndrome toxin 1 (TSST-1), particularly under low-oxygen conditions. In this study we investigated the localization and membrane orientation of SrrA and SrrB, transcription of the srrAB operon, the DNA-binding properties of SrrA, and the effect of SrrAB expression on S. aureus virulence. We found that SrrA is localized to the S. aureus cytoplasm, while SrrB is localized to the membrane and is properly oriented to function as a histidine kinase. srrAB has one transcriptional start site which results in either an srrA transcript or a full-length srrAB transcript; srrB must be cotranscribed with srrA. Gel shift assays of the agr P2, agr P3, protein A (spa), TSST-1 (tst), and srr promoters revealed SrrA binding at each of these promoters. Analysis of SrrAB-overexpressing strains by using the rabbit model of bacterial endocarditis demonstrated that overexpression of SrrAB decreased the virulence of the organisms compared to the virulence of isogenic strains that do not overexpress SrrAB. We concluded that SrrAB is properly localized and oriented to function as a two-component system. Overexpression of SrrAB, which represses agr RNAIII, TSST-1, and protein A in vitro, decreases virulence in the rabbit endocarditis model. Repression of these virulence factors is likely due to a direct interaction between SrrA and the agr, tst, and spa promoters.

  12. ResDE Two-Component Regulatory System Mediates Oxygen Limitation-Induced Biofilm Formation by Bacillus amyloliquefaciens SQR9.

    Science.gov (United States)

    Zhou, Xuan; Zhang, Nan; Xia, Liming; Li, Qing; Shao, Jiahui; Shen, Qirong; Zhang, Ruifu

    2018-04-15

    Efficient biofilm formation and root colonization capabilities facilitate the ability of beneficial plant rhizobacteria to promote plant growth and antagonize soilborne pathogens. Biofilm formation by plant-beneficial Bacillus strains is triggered by environmental cues, including oxygen deficiency, but the pathways that sense these environmental signals and regulate biofilm formation have not been thoroughly elucidated. In this study, we showed that the ResDE two-component regulatory system in the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens strain SQR9 senses the oxygen deficiency signal and regulates biofilm formation. ResE is activated by sensing the oxygen limitation-induced reduction of the NAD + /NADH pool through its PAS domain, stimulating its kinase activity, and resulting in the transfer of a phosphoryl group to ResD. The phosphorylated ResD directly binds to the promoter regions of the qoxABCD and ctaCDEF operons to improve the biosynthesis of terminal oxidases, which can interact with KinB to activate biofilm formation. These results not only revealed the novel regulatory function of the ResDE two-component system but also contributed to the understanding of the complicated regulatory network governing Bacillus biofilm formation. This research may help to enhance the root colonization and the plant-beneficial efficiency of SQR9 and other Bacillus rhizobacteria used in agriculture. IMPORTANCE Bacillus spp. are widely used as bioinoculants for plant growth promotion and disease suppression. The exertion of their plant-beneficial functions is largely dependent on their root colonization, which is closely related to their biofilm formation capabilities. On the other hand, Bacillus is the model bacterium for biofilm study, and the process and molecular network of biofilm formation are well characterized (B. Mielich-Süss and D. Lopez, Environ Microbiol 17:555-565, 2015, https://doi.org/10.1111/1462-2920.12527; L. S. Cairns, L. Hobley, and

  13. A connecter-like factor, CacA, links RssB/RpoS and the CpxR/CpxA two-component system in Salmonella.

    Science.gov (United States)

    Kato, Akinori; Hayashi, Hironori; Nomura, Wataru; Emori, Haruka; Hagihara, Kei; Utsumi, Ryutaro

    2012-10-02

    Bacteria integrate numerous environmental stimuli when generating cellular responses. Increasing numbers of examples describe how one two-component system (TCS) responds to signals detected by the sensor of another TCS. However, the molecular mechanisms underlying this phenomenon remain poorly defined. Here, we report a connector-like factor that affects the activity of the CpxR/CpxA two-component system in Salmonella enterica serovar Typhimurium. We isolated a clone that induced the expression of a cpxP-lac gene fusion from a high-copy-number plasmid pool of random Salmonella genomic fragments. A 63-amino acid protein, CacA, was responsible for the CpxA/CpxR-dependent activation of the cpxP gene. The CpxR-activated genes cpxP and spy exhibited approximately 30% and 50% reductions in transcription, respectively, in a clean cacA deletion mutant strain in comparison to wild-type. From 33 response regulator (RR) deletion mutants, we identified that the RssB regulator represses cacA transcription. Substitution mutations in a conserved -10 region harboring the RNA polymerase recognition sequence, which is well conserved with a known RpoS -10 region consensus sequence, rendered the cacA promoter RpoS-independent. The CacA-mediated induction of cpxP transcription was affected in a trxA deletion mutant, which encodes thioredoxin 1, suggesting a role for cysteine thiol-disulfide exchange(s) in CacA-dependent Cpx activation. We identified CacA as an activator of the CpxR/CpxA system in the plasmid clone. We propose that CacA may integrate the regulatory status of RssB/RpoS into the CpxR/CpxA system. Future investigations are necessary to thoroughly elucidate how CacA activates the CpxR/CpxA system.

  14. A connecter-like factor, CacA, links RssB/RpoS and the CpxR/CpxA two-component system in Salmonella

    Directory of Open Access Journals (Sweden)

    Kato Akinori

    2012-10-01

    Full Text Available Abstract Background Bacteria integrate numerous environmental stimuli when generating cellular responses. Increasing numbers of examples describe how one two-component system (TCS responds to signals detected by the sensor of another TCS. However, the molecular mechanisms underlying this phenomenon remain poorly defined. Results Here, we report a connector-like factor that affects the activity of the CpxR/CpxA two-component system in Salmonella enterica serovar Typhimurium. We isolated a clone that induced the expression of a cpxP-lac gene fusion from a high-copy-number plasmid pool of random Salmonella genomic fragments. A 63-amino acid protein, CacA, was responsible for the CpxA/CpxR-dependent activation of the cpxP gene. The CpxR-activated genes cpxP and spy exhibited approximately 30% and 50% reductions in transcription, respectively, in a clean cacA deletion mutant strain in comparison to wild-type. From 33 response regulator (RR deletion mutants, we identified that the RssB regulator represses cacA transcription. Substitution mutations in a conserved -10 region harboring the RNA polymerase recognition sequence, which is well conserved with a known RpoS -10 region consensus sequence, rendered the cacA promoter RpoS-independent. The CacA-mediated induction of cpxP transcription was affected in a trxA deletion mutant, which encodes thioredoxin 1, suggesting a role for cysteine thiol-disulfide exchange(s in CacA-dependent Cpx activation. Conclusions We identified CacA as an activator of the CpxR/CpxA system in the plasmid clone. We propose that CacA may integrate the regulatory status of RssB/RpoS into the CpxR/CpxA system. Future investigations are necessary to thoroughly elucidate how CacA activates the CpxR/CpxA system.

  15. A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003.

    Science.gov (United States)

    Alvarez-Martin, Pablo; Fernández, Matilde; O'Connell-Motherway, Mary; O'Connell, Kerry Joan; Sauvageot, Nicolas; Fitzgerald, Gerald F; MacSharry, John; Zomer, Aldert; van Sinderen, Douwe

    2012-08-01

    This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P(i)) starvation in Bifidobacterium breve UCC2003. The response regulator PhoP was shown to bind to the promoter region of pstSCAB, specifying a predicted P(i) transporter system, as well as that of phoU, which encodes a putative P(i)-responsive regulatory protein. This interaction is assumed to cause transcriptional modulation under conditions of P(i) limitation. Our data suggest that the phoRP genes are subject to positive autoregulation and, together with pstSCAB and presumably phoU, represent the complete regulon controlled by the phoRP-encoded 2CRS in B. breve UCC2003. Determination of the minimal PhoP binding region combined with bioinformatic analysis revealed the probable recognition sequence of PhoP, designated here as the PHO box, which together with phoRP is conserved among many high-GC-content Gram-positive bacteria. The importance of the phoRP 2CRS in the response of B. breve to P(i) starvation conditions was confirmed by analysis of a B. breve phoP insertion mutant which exhibited decreased growth under phosphate-limiting conditions compared to its parent strain UCC2003.

  16. The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate

    Science.gov (United States)

    2009-01-01

    Background Secondary metabolism in Serratia sp. ATCC 39006 (Serratia 39006) is controlled via a complex network of regulators, including a LuxIR-type (SmaIR) quorum sensing (QS) system. Here we investigate the molecular mechanism by which phosphate limitation controls biosynthesis of two antibiotic secondary metabolites, prodigiosin and carbapenem, in Serratia 39006. Results We demonstrate that a mutation in the high affinity phosphate transporter pstSCAB-phoU, believed to mimic low phosphate conditions, causes upregulation of secondary metabolism and QS in Serratia 39006, via the PhoBR two-component system. Phosphate limitation also activated secondary metabolism and QS in Serratia 39006. In addition, a pstS mutation resulted in upregulation of rap. Rap, a putative SlyA/MarR-family transcriptional regulator, shares similarity with the global regulator RovA (regulator of virulence) from Yersina spp. and is an activator of secondary metabolism in Serratia 39006. We demonstrate that expression of rap, pigA-O (encoding the prodigiosin biosynthetic operon) and smaI are controlled via PhoBR in Serratia 39006. Conclusion Phosphate limitation regulates secondary metabolism in Serratia 39006 via multiple inter-linked pathways, incorporating transcriptional control mediated by three important global regulators, PhoB, SmaR and Rap. PMID:19476633

  17. The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate

    Directory of Open Access Journals (Sweden)

    Everson Lee

    2009-05-01

    Full Text Available Abstract Background Secondary metabolism in Serratia sp. ATCC 39006 (Serratia 39006 is controlled via a complex network of regulators, including a LuxIR-type (SmaIR quorum sensing (QS system. Here we investigate the molecular mechanism by which phosphate limitation controls biosynthesis of two antibiotic secondary metabolites, prodigiosin and carbapenem, in Serratia 39006. Results We demonstrate that a mutation in the high affinity phosphate transporter pstSCAB-phoU, believed to mimic low phosphate conditions, causes upregulation of secondary metabolism and QS in Serratia 39006, via the PhoBR two-component system. Phosphate limitation also activated secondary metabolism and QS in Serratia 39006. In addition, a pstS mutation resulted in upregulation of rap. Rap, a putative SlyA/MarR-family transcriptional regulator, shares similarity with the global regulator RovA (regulator of virulence from Yersina spp. and is an activator of secondary metabolism in Serratia 39006. We demonstrate that expression of rap, pigA-O (encoding the prodigiosin biosynthetic operon and smaI are controlled via PhoBR in Serratia 39006. Conclusion Phosphate limitation regulates secondary metabolism in Serratia 39006 via multiple inter-linked pathways, incorporating transcriptional control mediated by three important global regulators, PhoB, SmaR and Rap.

  18. A Two-Component Regulatory System Impacts Extracellular Membrane-Derived Vesicle Production in Group A Streptococcus

    Directory of Open Access Journals (Sweden)

    Ulrike Resch

    2016-11-01

    Full Text Available Export of macromolecules via extracellular membrane-derived vesicles (MVs plays an important role in the biology of Gram-negative bacteria. Gram-positive bacteria have also recently been reported to produce MVs; however, the composition and mechanisms governing vesiculogenesis in Gram-positive bacteria remain undefined. Here, we describe MV production in the Gram-positive human pathogen group A streptococcus (GAS, the etiological agent of necrotizing fasciitis and streptococcal toxic shock syndrome. M1 serotype GAS isolates in culture exhibit MV structures both on the cell wall surface and in the near vicinity of bacterial cells. A comprehensive analysis of MV proteins identified both virulence-associated protein substrates of the general secretory pathway in addition to “anchorless surface proteins.” Characteristic differences in the contents, distributions, and fatty acid compositions of specific lipids between MVs and GAS cell membrane were also observed. Furthermore, deep RNA sequencing of vesicular RNAs revealed that GAS MVs contained differentially abundant RNA species relative to bacterial cellular RNA. MV production by GAS strains varied in a manner dependent on an intact two-component system, CovRS, with MV production negatively regulated by the system. Modulation of MV production through CovRS was found to be independent of both GAS cysteine protease SpeB and capsule biosynthesis. Our data provide an explanation for GAS secretion of macromolecules, including RNAs, lipids, and proteins, and illustrate a regulatory mechanism coordinating this secretory response.

  19. The NtrY-NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1.

    Science.gov (United States)

    Bonato, Paloma; Alves, Lysangela R; Osaki, Juliana H; Rigo, Liu U; Pedrosa, Fabio O; Souza, Emanuel M; Zhang, Nan; Schumacher, Jörg; Buck, Martin; Wassem, Roseli; Chubatsu, Leda S

    2016-11-01

    Herbaspirillum seropedicae is a diazotrophic β-Proteobacterium found endophytically associated with gramineae (Poaceae or graminaceous plants) such as rice, sorghum and sugar cane. In this work we show that nitrate-dependent growth in this organism is regulated by the master nitrogen regulatory two-component system NtrB-NtrC, and by NtrY-NtrX, which functions to specifically regulate nitrate metabolism. NtrY is a histidine kinase sensor protein predicted to be associated with the membrane and NtrX is the response regulator partner. The ntrYntrX genes are widely distributed in Proteobacteria. In α-Proteobacteria they are frequently located downstream from ntrBC, whereas in β-Proteobacteria these genes are located downstream from genes encoding an RNA methyltransferase and a proline-rich protein with unknown function. The NtrX protein of α-Proteobacteria has an AAA+ domain, absent in those from β-Proteobacteria. An ntrY mutant of H. seropedicae showed the wild-type nitrogen fixation phenotype, but the nitrate-dependent growth was abolished. Gene fusion assays indicated that NtrY is involved in the expression of genes coding for the assimilatory nitrate reductase as well as the nitrate-responsive two-component system NarX-NarL (narK and narX promoters, respectively). The purified NtrX protein was capable of binding the narK and narX promoters, and the binding site at the narX promoter for the NtrX protein was determined by DNA footprinting. In silico analyses revealed similar sequences in other promoter regions of H. seropedicae that are related to nitrate assimilation, supporting the role of the NtrY-NtrX system in regulating nitrate metabolism in H. seropedicae. © 2016 Federation of European Biochemical Societies.

  20. Gene expression of a two-component regulatory system associated with sunscreen biosynthesis in the cyanobacterium Nostoc punctiforme ATCC 29133.

    Science.gov (United States)

    Janssen, Jacob; Soule, Tanya

    2016-01-01

    Long-wavelength ultraviolet radiation (UVA) can damage cells through photooxidative stress, leading to harmful photosensitized proteins and pigments in cyanobacteria. To mitigate damage, some cyanobacteria secrete the UVA-absorbing pigment scytonemin into their extracellular sheath. Comparative genomic analyses suggest that scytonemin biosynthesis is regulated by the two-component regulatory system (TCRS) proteins encoded by Npun_F1277 and Npun_F1278 in the cyanobacterium Nostoc punctiforme ATCC 29133. To understand the dynamics of these genes, their expression was measured following exposure to UVA, UVB, high visible (VIS) irradiance and oxidative stress for 20, 40 and 60 min. Overall, both genes had statistically similar patterns of expression for all four conditions and were generally upregulated, except for those exposed to UVB by 60 min and for the cells under oxidative stress. The greatest UVA response was an upregulation by 20 min, while the response to UVB was the most dramatic and persisted through 40 min. High VIS irradiance resulted in a modest upregulation, while oxidative stress caused a slight downregulation. Both genes were also found to occur on the same transcript. These results demonstrate that these genes are positively responding to several light-associated conditions, which suggests that this TCRS may regulate more than just scytonemin biosynthesis under UVA stress. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. Haemophilus parasuis CpxRA two-component system confers bacterial tolerance to environmental stresses and macrolide resistance.

    Science.gov (United States)

    Cao, Qi; Feng, Fenfen; Wang, Huan; Xu, Xiaojuan; Chen, Huanchun; Cai, Xuwang; Wang, Xiangru

    2018-01-01

    Haemophilus parasuis is an opportunistic pathogen localized in the upper respiratory tracts of pigs, its infection begins from bacterial survival under complex conditions, like hyperosmosis, oxidative stress, phagocytosis, and sometimes antibiotics as well. The two-component signal transduction (TCST) system serves as a common stimulus-response mechanism that allows microbes to sense and respond to diverse environmental conditions via a series of phosphorylation reactions. In this study, we investigated the role of TCST system CpxRA in H. parasuis in response to different environmental stimuli by constructing the ΔcpxA and ΔcpxR single deletion mutants as well as the ΔcpxRA double deletion mutant from H. parasuis serotype 4 isolate JS0135. We demonstrated that H. parasuis TCST system CpxRA confers bacterial tolerance to stresses and bactericidal antibiotics. The CpxR was found to play essential roles in mediating oxidative stress, osmotic stresses and alkaline pH stress tolerance, as well as macrolide resistance (i.e. erythromycin), but the CpxA deletion did not decrease bacterial resistance to abovementioned stresses. Moreover, we found via RT-qPCR approach that HAPS_RS00160 and HAPS_RS09425, both encoding multidrug efflux pumps, were significantly decreased in erythromycin challenged ΔcpxR and ΔcpxRA mutants compared with wild-type strain JS0135. These findings characterize the role of the TCST system CpxRA in H. parasuis conferring stress response tolerance and bactericidal resistance, which will deepen our understanding of the pathogenic mechanism in H. parasuis. Copyright © 2017 Elsevier GmbH. All rights reserved.

  2. Systems level analysis of two-component signal transduction systems in Erwinia amylovora: Role in virulence, regulation of amylovoran biosynthesis and swarming motility

    Directory of Open Access Journals (Sweden)

    Sundin George W

    2009-05-01

    Full Text Available Abstract Background Two-component signal transduction systems (TCSTs, consisting of a histidine kinase (HK and a response regulator (RR, represent a major paradigm for signal transduction in prokaryotes. TCSTs play critical roles in sensing and responding to environmental conditions, and in bacterial pathogenesis. Most TCSTs in Erwinia amylovora have either not been identified or have not yet been studied. Results We used a systems approach to identify TCST and related signal transduction genes in the genome of E. amylovora. Comparative genomic analysis of TCSTs indicated that E. amylovora TCSTs were closely related to those of Erwinia tasmaniensis, a saprophytic enterobacterium isolated from apple flowers, and to other enterobacteria. Forty-six TCST genes in E. amylovora including 17 sensor kinases, three hybrid kinases, 20 DNA- or ligand-binding RRs, four RRs with enzymatic output domain (EAL-GGDEF proteins, and two kinases were characterized in this study. A systematic TCST gene-knockout experiment was conducted, generating a total of 59 single-, double-, and triple-mutants. Virulence assays revealed that five of these mutants were non-pathogenic on immature pear fruits. Results from phenotypic characterization and gene expression experiments indicated that several groups of TCST systems in E. amylovora control amylovoran biosynthesis, one of two major virulence factors in E. amylovora. Both negative and positive regulators of amylovoran biosynthesis were identified, indicating a complex network may control this important feature of pathogenesis. Positive (non-motile, EnvZ/OmpR, negative (hypermotile, GrrS/GrrA, and intermediate regulators for swarming motility in E. amylovora were also identified. Conclusion Our results demonstrated that TCSTs in E. amylovora played major roles in virulence on immature pear fruit and in regulating amylovoran biosynthesis and swarming motility. This suggested presence of regulatory networks governing

  3. Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation

    Science.gov (United States)

    2011-01-01

    Background Staphylococcus epidermidis (SE) has emerged as one of the most important causes of nosocomial infections. The SaeRS two-component signal transduction system (TCS) influences virulence and biofilm formation in Staphylococcus aureus. The deletion of saeR in S. epidermidis results in impaired anaerobic growth and decreased nitrate utilization. However, the regulatory function of SaeRS on biofilm formation and autolysis in S. epidermidis remains unclear. Results The saeRS genes of SE1457 were deleted by homologous recombination. The saeRS deletion mutant, SE1457ΔsaeRS, exhibited increased biofilm formation that was disturbed more severely (a 4-fold reduction) by DNase I treatment compared to SE1457 and the complementation strain SE1457saec. Compared to SE1457 and SE1457saec, SE1457ΔsaeRS showed increased Triton X-100-induced autolysis (approximately 3-fold) and decreased cell viability in planktonic/biofilm states; further, SE1457ΔsaeRS also released more extracellular DNA (eDNA) in the biofilms. Correlated with the increased autolysis phenotype, the transcription of autolysis-related genes, such as atlE and aae, was increased in SE1457ΔsaeRS. Whereas the expression of accumulation-associated protein was up-regulated by 1.8-fold in 1457ΔsaeRS, the expression of an N-acetylglucosaminyl transferase enzyme (encoded by icaA) critical for polysaccharide intercellular adhesin (PIA) synthesis was not affected by the deletion of saeRS. Conclusions Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457ΔsaeRS was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states. PMID:21702925

  4. Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation

    Directory of Open Access Journals (Sweden)

    Francois Patrice

    2011-06-01

    Full Text Available Abstract Background Staphylococcus epidermidis (SE has emerged as one of the most important causes of nosocomial infections. The SaeRS two-component signal transduction system (TCS influences virulence and biofilm formation in Staphylococcus aureus. The deletion of saeR in S. epidermidis results in impaired anaerobic growth and decreased nitrate utilization. However, the regulatory function of SaeRS on biofilm formation and autolysis in S. epidermidis remains unclear. Results The saeRS genes of SE1457 were deleted by homologous recombination. The saeRS deletion mutant, SE1457ΔsaeRS, exhibited increased biofilm formation that was disturbed more severely (a 4-fold reduction by DNase I treatment compared to SE1457 and the complementation strain SE1457saec. Compared to SE1457 and SE1457saec, SE1457ΔsaeRS showed increased Triton X-100-induced autolysis (approximately 3-fold and decreased cell viability in planktonic/biofilm states; further, SE1457ΔsaeRS also released more extracellular DNA (eDNA in the biofilms. Correlated with the increased autolysis phenotype, the transcription of autolysis-related genes, such as atlE and aae, was increased in SE1457ΔsaeRS. Whereas the expression of accumulation-associated protein was up-regulated by 1.8-fold in 1457ΔsaeRS, the expression of an N-acetylglucosaminyl transferase enzyme (encoded by icaA critical for polysaccharide intercellular adhesin (PIA synthesis was not affected by the deletion of saeRS. Conclusions Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457ΔsaeRS was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states.

  5. Two-component system VicRK regulates functions associated with establishment of Streptococcus sanguinis in biofilms.

    Science.gov (United States)

    Moraes, Julianna J; Stipp, Rafael N; Harth-Chu, Erika N; Camargo, Tarsila M; Höfling, José F; Mattos-Graner, Renata O

    2014-12-01

    Streptococcus sanguinis is a commensal pioneer colonizer of teeth and an opportunistic pathogen of infectious endocarditis. The establishment of S. sanguinis in host sites likely requires dynamic fitting of the cell wall in response to local stimuli. In this study, we investigated the two-component system (TCS) VicRK in S. sanguinis (VicRKSs), which regulates genes of cell wall biogenesis, biofilm formation, and virulence in opportunistic pathogens. A vicK knockout mutant obtained from strain SK36 (SKvic) showed slight reductions in aerobic growth and resistance to oxidative stress but an impaired ability to form biofilms, a phenotype restored in the complemented mutant. The biofilm-defective phenotype was associated with reduced amounts of extracellular DNA during aerobic growth, with reduced production of H2O2, a metabolic product associated with DNA release, and with inhibitory capacity of S. sanguinis competitor species. No changes in autolysis or cell surface hydrophobicity were detected in SKvic. Reverse transcription-quantitative PCR (RT-qPCR), electrophoretic mobility shift assays (EMSA), and promoter sequence analyses revealed that VicR directly regulates genes encoding murein hydrolases (SSA_0094, cwdP, and gbpB) and spxB, which encodes pyruvate oxidase for H2O2 production. Genes previously associated with spxB expression (spxR, ccpA, ackA, and tpK) were not transcriptionally affected in SKvic. RT-qPCR analyses of S. sanguinis biofilm cells further showed upregulation of VicRK targets (spxB, gbpB, and SSA_0094) and other genes for biofilm formation (gtfP and comE) compared to expression in planktonic cells. This study provides evidence that VicRKSs regulates functions crucial for S. sanguinis establishment in biofilms and identifies novel VicRK targets potentially involved in hydrolytic activities of the cell wall required for these functions. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  6. Novel Two-Component System of Streptococcus sanguinis Affecting Functions Associated with Viability in Saliva and Biofilm Formation.

    Science.gov (United States)

    Camargo, Tarsila M; Stipp, Rafael N; Alves, Lívia A; Harth-Chu, Erika N; Höfling, José F; Mattos-Graner, Renata O

    2018-04-01

    Streptococcus sanguinis is a pioneer species of teeth and a common opportunistic pathogen of infective endocarditis. In this study, we identified a two-component system, S. sanguinis SptRS (SptRS Ss ), affecting S. sanguinis survival in saliva and biofilm formation. Isogenic mutants of sptR Ss (SKsptR) and sptS Ss (SKsptS) showed reduced cell counts in ex vivo assays of viability in saliva compared to those of parent strain SK36 and complemented mutants. Reduced counts of the mutants in saliva were associated with reduced growth rates in nutrient-poor medium (RPMI) and increased susceptibility to the deposition of C3b and the membrane attach complex (MAC) of the complement system, a defense component of saliva and serum. Conversely, sptR Ss and sptS Ss mutants showed increased biofilm formation associated with higher levels of production of H 2 O 2 and extracellular DNA. Reverse transcription-quantitative PCR (RT-qPCR) comparisons of strains indicated a global role of SptRS Ss in repressing genes for H 2 O 2 production (2.5- to 15-fold upregulation of spxB , spxR , vicR , tpk , and ackA in sptR Ss and sptS Ss mutants), biofilm formation, and/or evasion of host immunity (2.1- to 11.4-fold upregulation of srtA , pcsB , cwdP , iga , and nt5e ). Compatible with the homology of SptR Ss with AraC-type regulators, duplicate to multiple conserved repeats were identified in 1,000-bp regulatory regions of downstream genes, suggesting that SptR Ss regulates transcription by DNA looping. Significant transcriptional changes in the regulatory genes vicR , spxR , comE , comX , and mecA in the sptR Ss and sptS Ss mutants further indicated that SptRS Ss is part of a regulatory network that coordinates cell wall homeostasis, H 2 O 2 production, and competence. This study reveals that SptRS Ss is involved in the regulation of crucial functions for S. sanguinis persistence in the oral cavity. Copyright © 2018 American Society for Microbiology.

  7. Fluorescence lifetime selectivity in excitation-emission matrices for qualitative analysis of a two-component system

    International Nuclear Information System (INIS)

    Millican, D.W.; McGown, L.B.

    1989-01-01

    Steady-state fluorescence excitation-emission matrices (EEMs), and phase-resolved EEMs (PREEMs) collected at modulation frequencies of 6, 18, and 30 MHz, were used for qualitative analysis of mixtures of benzo[k]fluoranthene (τ = 8 ns) and benzo[b]fluoranthene (τ = 29 ns) in ethanol. The EEMs of the individual components were extracted from mixture EEMs by means of wavelength component vector-gram (WCV) analysis. Phase resolution was found to be superior to steady-state measurements for extraction of the component spectra, for mixtures in which the intensity contributions from the two components are unequal

  8. A conserved two-component regulatory system, PidS/PidR, globally regulates pigmentation and virulence-related phenotypes of Burkholderia glumae.

    Science.gov (United States)

    Karki, Hari Sharan; Barphagha, Inderjit Kaur; Ham, Jong Hyun

    2012-09-01

    Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B. glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B. glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, the PidS/PidR TCRS was also required for the elicitation of the hypersensitive response on tobacco leaves, indicating the dependence of the hypersensitive response and pathogenicity (Hrp) type III secretion system of B. glumae on this regulatory factor. In addition, B. glumae mutants defective in the PidS/PidR TCRS showed less production of the phytotoxin, toxoflavin, and less virulence on rice panicles and onion bulbs relative to the parental strain, 411gr-6. The presence of highly homologous PidS and PidR orthologues in other Burkholderia species suggests that PidS/PidR-family TCRSs may exert the same or similar functions in different Burkholderia species, including both plant and animal pathogens. © 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD.

  9. The two-component system CpxRA negatively regulates the Locus of Enterocyte Effacement of enterohemorrhagic Escherichia coli involving sigma 32 and Lon protease

    Directory of Open Access Journals (Sweden)

    MIGUEL A. eDE LA CRUZ

    2016-02-01

    Full Text Available Enterohemorrhagic Escherichia coli (EHEC is a significant cause of serious human gastrointestinal disease worldwide. EHEC strains contain a pathogenicity island called the locus of enterocyte effacement (LEE, which encodes virulence factors responsible for damaging the gut mucosa. The Cpx envelope stress response of E. coli is controlled by a two-component system consisting of a sensor histidine kinase (CpxA and a cytoplasmic response regulator (CpxR. In this study, we investigated the role of CpxRA in the expression of LEE-encoded virulence factors of EHEC. We found that a mutation in cpxA significantly affected adherence of EHEC to human epithelial cells. Analysis of this mutant revealed the presence of high levels of CpxR which repressed transcription of grlA and ler, the main positive virulence regulators of the LEE, and influenced negatively the production of the type 3 secretion system–associated EspABD translocator proteins. It is known that CpxR activates rpoH (Sigma factor 32, which in turns activates transcription of the lon protease gene. We found that transcription levels of ler and grlA were significantly increased in the lon and cpxA lon mutants suggesting that lon is involved in down-regulating LEE genes. In addition, the Galleria mellonella model of infection was used to analyze the effect of the loss of the cpx and lon genes in EHEC’s ability to kill the larvae. We found that the cpxA mutant was significantly deficient at killing the larvae however, the cpxA lon mutant which overexpresses LEE genes in vitro, was unable to kill the larvae, suggesting that virulence in the G. mellonella model is T3SS independent and that CpxA modulates virulence through a yet unknown EHEC-specific factor. Our data provides new insights and broadens our scope into the complex regulatory network of the LEE in which the CpxA sensor kinase plays an important role in a cascade involving both global and virulence regulators.

  10. A Two-Component Regulatory System, CsrR-CsrS, Represses Expression of Three Streptococcus pyogenes Virulence Factors, Hyaluronic Acid Capsule, Streptolysin S, and Pyrogenic Exotoxin B

    OpenAIRE

    Heath, Andrew; DiRita, Victor J.; Barg, Neil L.; Engleberg, N. Cary

    1999-01-01

    Certain Tn916 insertions in the chromosome of an M1-type, nonmucoid Streptococcus pyogenes isolate (MGAS166) were previously shown to result in stable mucoidy with increased expression of the capsular synthetic genes. The transposon insertions in these strains are directly upstream of an apparent operon encoding a two-component regulatory system, designated csrR-csrS. Compared with MGAS166, these mucoid mutants are more hemolytic and cause significantly more tissue damage in a murine model of...

  11. Towards Sensor Database Systems

    DEFF Research Database (Denmark)

    Bonnet, Philippe; Gehrke, Johannes; Seshadri, Praveen

    2001-01-01

    . These systems lack flexibility because data is extracted in a predefined way; also, they do not scale to a large number of devices because large volumes of raw data are transferred regardless of the queries that are submitted. In our new concept of sensor database system, queries dictate which data is extracted...... from the sensors. In this paper, we define the concept of sensor databases mixing stored data represented as relations and sensor data represented as time series. Each long-running query formulated over a sensor database defines a persistent view, which is maintained during a given time interval. We...... also describe the design and implementation of the COUGAR sensor database system....

  12. Geometrical Description of Chemical Equilibrium and Le Cha^telier's Principle: Two-Component Systems

    Science.gov (United States)

    Novak, Igor

    2018-01-01

    Chemical equilibrium is one of the most important concepts in chemistry. The changes in properties of the chemical system at equilibrium induced by variations in pressure, volume, temperature, and concentration are always included in classroom teaching and discussions. This work introduces a novel, geometrical approach to understanding the…

  13. The Vibrio cholerae VprA-VprB Two-Component System Controls Virulence Through Endotoxin Modification

    Science.gov (United States)

    2014-12-23

    concomitant reduction in glycine-lipid A modifica- tion. Wild-type cells grown in the presence of iron Fe3 (40 M), however, did not show a significant...CaCl2, 0.015 mM FeSO4, 0.075 mM thiamine, and 0.15 mM KH2PO4 (20, 66). In all cases, cells were grown with aeration at 37°C. E. coli strains were...dephosphory- lated and labeled with [-32P]ATP (Amersham) using a DNA 5=-end- labeling system (Promega). Removal of unincorporated nucleotides was carried out

  14. Polymyxin resistance of Pseudomonas aeruginosa phoQ mutants is dependent on additional two-component regulatory systems

    DEFF Research Database (Denmark)

    Gutu, Alina D; Sgambati, Nicole; Strasbourger, Pnina

    2013-01-01

    Pseudomonas aeruginosa can develop resistance to polymyxin as a consequence of mutations in the PhoPQ regulatory system, mediated by covalent lipid A modification. Transposon mutagenesis of a polymyxin-resistant phoQ mutant defined 41 novel loci required for resistance, including two regulatory s......, indicate that addition of 4-amino-L-arabinose to lipid A is not the only PhoPQ-regulated biochemical mechanism required for resistance, and demonstrate that colRS and cprS mutations can contribute to high-level clinical resistance....... with the known role of this modification in polymyxin resistance. Surprisingly, tandem deletion of colRS or cprRS in the ΔphoQ mutant or individual deletion of cprR or cprS failed to suppress 4-amino-L-arabinose addition to lipid A, indicating that this modification alone is not sufficient for Pho...

  15. Immediate and heterogeneous response of the LiaFSR two-component system of Bacillus subtilis to the peptide antibiotic bacitracin.

    Science.gov (United States)

    Kesel, Sara; Mader, Andreas; Höfler, Carolin; Mascher, Thorsten; Leisner, Madeleine

    2013-01-01

    Two-component signal transduction systems are one means of bacteria to respond to external stimuli. The LiaFSR two-component system of Bacillus subtilis consists of a regular two-component system LiaRS comprising the core Histidine Kinase (HK) LiaS and the Response Regulator (RR) LiaR and additionally the accessory protein LiaF, which acts as a negative regulator of LiaRS-dependent signal transduction. The complete LiaFSR system was shown to respond to various peptide antibiotics interfering with cell wall biosynthesis, including bacitracin. Here we study the response of the LiaFSR system to various concentrations of the peptide antibiotic bacitracin. Using quantitative fluorescence microscopy, we performed a whole population study analyzed on the single cell level. We investigated switching from the non-induced 'OFF' state into the bacitracin-induced 'ON' state by monitoring gene expression of a fluorescent reporter from the RR-regulated liaI promoter. We found that switching into the 'ON' state occurred within less than 20 min in a well-defined switching window, independent of the bacitracin concentration. The switching rate and the basal expression rate decreased at low bacitracin concentrations, establishing clear heterogeneity 60 min after bacitracin induction. Finally, we performed time-lapse microscopy of single cells confirming the quantitative response as obtained in the whole population analysis for high bacitracin concentrations. The LiaFSR system exhibits an immediate, heterogeneous and graded response to the inducer bacitracin in the exponential growth phase.

  16. A novel outer-membrane anion channel (porin) as part of a putatively two-component transport system for 4-toluenesulphonate in Comamonas testosteroni T-2

    OpenAIRE

    Mampel, Jörg; Maier, Elke; Tralau, Tewes; Ruff, Jürgen; Benz, Roland; Cook, Alasdair M.

    2004-01-01

    Inducible mineralization of TSA (4-toluenesulphonate) by Comamonas testosteroni T-2 is initiated by a secondary transport system, followed by oxygenation and oxidation by TsaMBCD to 4-sulphobenzoate under the regulation of TsaR and TsaQ. Evidence is presented for a novel, presumably two-component transport system (TsaST). It is proposed that TsaT, an outer-membrane porin, formed an anion-selective channel that works in co-operation with the putative secondary transporter, TsaS, located in the...

  17. On the characterization of dynamic supramolecular systems: a general mathematical association model for linear supramolecular copolymers and application on a complex two-component hydrogen-bonding system.

    Science.gov (United States)

    Odille, Fabrice G J; Jónsson, Stefán; Stjernqvist, Susann; Rydén, Tobias; Wärnmark, Kenneth

    2007-01-01

    A general mathematical model for the characterization of the dynamic (kinetically labile) association of supramolecular assemblies in solution is presented. It is an extension of the equal K (EK) model by the stringent use of linear algebra to allow for the simultaneous presence of an unlimited number of different units in the resulting assemblies. It allows for the analysis of highly complex dynamic equilibrium systems in solution, including both supramolecular homo- and copolymers without the recourse to extensive approximations, in a field in which other analytical methods are difficult. The derived mathematical methodology makes it possible to analyze dynamic systems such as supramolecular copolymers regarding for instance the degree of polymerization, the distribution of a given monomer in different copolymers as well as its position in an aggregate. It is to date the only general means to characterize weak supramolecular systems. The model was fitted to NMR dilution titration data by using the program Matlab, and a detailed algorithm for the optimization of the different parameters has been developed. The methodology is applied to a case study, a hydrogen-bonded supramolecular system, salen 4+porphyrin 5. The system is formally a two-component system but in reality a three-component system. This results in a complex dynamic system in which all monomers are associated to each other by hydrogen bonding with different association constants, resulting in homo- and copolymers 4n5m as well as cyclic structures 6 and 7, in addition to free 4 and 5. The system was analyzed by extensive NMR dilution titrations at variable temperatures. All chemical shifts observed at different temperatures were used in the fitting to obtain the DeltaH degrees and DeltaS degrees values producing the best global fit. From the derived general mathematical expressions, system 4+5 could be characterized with respect to above-mentioned parameters.

  18. The Role of the Two-Component System BaeSR in Disposing Chemicals through Regulating Transporter Systems in Acinetobacter baumannii.

    Directory of Open Access Journals (Sweden)

    Ming-Feng Lin

    Full Text Available Bacterial two-component regulatory systems (TCSs facilitate changes in gene expression in response to environmental stimuli. TCS BaeR regulons influence tigecycline susceptibility in Acinetobacter baumannii through positively regulating the pump genes adeA and adeB. In this study, we demonstrate that an additional two transport systems, AdeIJK and MacAB-TolC, are also regulated by BaeSR. In the wild type and clinical tigecycline-resistant A. baumannii strains, gene expression of AdeIJK and MacAB-TolC increased after tigecycline induction, implicating their importance to tigecycline resistance in addition to AdeABC. Phenotypic microarray results showed that A. baumannii is vulnerable to certain chemicals, especially tannic acid, after deleting baeR, which was confirmed using the spot assay. The wild-type strain of A. baumannii also exhibited 1.6-fold and 4.4-fold increase in gene expression of adeJ and macB in the medium with 100 μg/mL tannic acid, but the increase was fully inhibited by baeR deletion. An electrophoretic motility shift assay based on an interaction between His-BaeR and the adeA, adeI and macA promoter regions did not demonstrate direct binding. In conclusion, A. baumannii can use the TCS BaeSR in disposing chemicals, such as tannic acid and tigecycline, through regulating the efflux pumps.

  19. Embedded sensor systems

    CERN Document Server

    Agrawal, Dharma Prakash

    2017-01-01

    This inspiring textbook provides an introduction to wireless technologies for sensors, explores potential use of sensors for numerous applications, and utilizes probability theory and mathematical methods as a means of embedding sensors in system design. It discusses the need for synchronization and underlying limitations, inter-relation between given coverage and connectivity to number of sensors needed, and the use of geometrical distance to determine location of the base station for data collection and explore use of anchor nodes for relative position determination of sensors. The book explores energy conservation, communication using TCP, the need for clustering and data aggregation, and residual energy determination and energy harvesting. It covers key topics of sensor communication like mobile base stations and relay nodes, delay-tolerant sensor networks, and remote sensing and possible applications. The book defines routing methods and do performance evaluation for random and regular sensor topology an...

  20. Control of CydB and GltA1 expression by the SenX3 RegX3 two component regulatory system of Mycobacterium tuberculosis.

    Directory of Open Access Journals (Sweden)

    Gretta Roberts

    Full Text Available Two component regulatory systems are used widely by bacteria to coordinate changes in global gene expression profiles in response to environmental signals. The SenX3-RegX3 two component system of Mycobacterium tuberculosis has previously been shown to play a role in virulence and phosphate-responsive control of gene expression. We demonstrate that expression of SenX3-RegX3 is controlled in response to growth conditions, although the absolute changes are small. Global gene expression profiling of a RegX3 deletion strain and wild-type strain in different culture conditions (static, microaerobic, anaerobic, as well as in an over-expressing strain identified a number of genes with changed expression patterns. Among those were genes previously identified as differentially regulated in aerobic culture, including ald (encoding alanine dehydrogenase cyd,encoding a subunit of the cytochrome D ubiquinol oxidase, and gltA1, encoding a citrate synthase. Promoter activity in the upstream regions of both cydB and gltA1 was altered in the RegX3 deletion strain. DNA-binding assays confirmed that RegX3 binds to the promoter regions of ald, cydB and gltA1 in a phosphorylation-dependent manner. Taken together these data suggest a direct role for the SenX-RegX3 system in modulating expression of aerobic respiration, in addition to its role during phosphate limitation.

  1. Quantum theory for the dynamic structure factor in correlated two-component systems in nonequilibrium: Application to x-ray scattering

    Science.gov (United States)

    Vorberger, J.; Chapman, D. A.

    2018-01-01

    We present a quantum theory for the dynamic structure factors in nonequilibrium, correlated, two-component systems such as plasmas or warm dense matter. The polarization function, which is needed as the input for the calculation of the structure factors, is calculated in nonequilibrium based on a perturbation expansion in the interaction strength. To make our theory applicable for x-ray scattering, a generalized Chihara decomposition for the total electron structure factor in nonequilibrium is derived. Examples are given and the influence of correlations and exchange on the structure and the x-ray-scattering spectrum are discussed for a model nonequilibrium distribution, as often encountered during laser heating of materials, as well as for two-temperature systems.

  2. Dual Two-Component Regulatory Systems Are Involved in Aromatic Compound Degradation in a Polychlorinated-Biphenyl Degrader, Rhodococcus jostii RHA1 ▿ †

    OpenAIRE

    Takeda, Hisashi; Shimodaira, Jun; Yukawa, Kiyoshi; Hara, Naho; Kasai, Daisuke; Miyauchi, Keisuke; Masai, Eiji; Fukuda, Masao

    2010-01-01

    A Gram-positive polychlorinated-biphenyl (PCB) degrader, Rhodococcus jostii RHA1, degrades PCBs by cometabolism with biphenyl. A two-component BphS1T1 system encoded by bphS1 and bphT1 (formerly bphS and bphT) is responsible for the transcription induction of the five gene clusters, bphAaAbAcAdC1B1, etbAa1Ab1CbphD1, etbAa2Ab2AcD2, etbAdbphB2, and etbD1, which constitute multiple enzyme systems for biphenyl/PCB degradation. The bphS2 and bphT2 genes, which encode BphS2 and BphT2, virtually ide...

  3. Quantum theory for the dynamic structure factor in correlated two-component systems in nonequilibrium: Application to x-ray scattering.

    Science.gov (United States)

    Vorberger, J; Chapman, D A

    2018-01-01

    We present a quantum theory for the dynamic structure factors in nonequilibrium, correlated, two-component systems such as plasmas or warm dense matter. The polarization function, which is needed as the input for the calculation of the structure factors, is calculated in nonequilibrium based on a perturbation expansion in the interaction strength. To make our theory applicable for x-ray scattering, a generalized Chihara decomposition for the total electron structure factor in nonequilibrium is derived. Examples are given and the influence of correlations and exchange on the structure and the x-ray-scattering spectrum are discussed for a model nonequilibrium distribution, as often encountered during laser heating of materials, as well as for two-temperature systems.

  4. Smart sensors and systems

    CERN Document Server

    Kyung, Chong-Min; Yasuura, Hiroto; Liu, Yongpan

    2015-01-01

     This book describes for readers technology used for effective sensing of our physical world and intelligent processing techniques for sensed information, which are essential to the success of Internet of Things (IoTs).  The authors provide a multidisciplinary view of sensor technology from MEMS, biological, chemical, and electrical domains and showcase smart sensor systems in real applications including smart home, transportation, medical, environmental, agricultural, etc.  Unlike earlier books on sensors, this book will provide a “global” view on smart sensors covering abstraction levels from device, circuit, systems, and algorithms.  .

  5. Bioinspired Sensor Systems

    Directory of Open Access Journals (Sweden)

    Manel del Valle

    2011-10-01

    Full Text Available This editorial summarizes and classifies the contributions presented by different authors to the special issue of the journal Sensors dedicated to Bioinspired Sensor Systems. From the coupling of sensor arrays or networks, plus computer processing abilities, new applications to mimic or to complement human senses are arising in the context of ambient intelligence. Principles used, and illustrative study cases have been presented permitting readers to grasp the current status of the field.

  6. Exposure of a 23F Serotype Strain of Streptococcus pneumoniae to Cigarette Smoke Condensate Is Associated with Selective Upregulation of Genes Encoding the Two-Component Regulatory System 11 (TCS11

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    Riana Cockeran

    2014-01-01

    Full Text Available Alterations in whole genome expression profiles following exposure of the pneumococcus (strain 172, serotype 23F to cigarette smoke condensate (160 μg/mL for 15 and 60 min have been determined using the TIGR4 DNA microarray chip. Exposure to CSC resulted in the significant (P<0.014–0.0006 upregulation of the genes encoding the two-component regulatory system 11 (TCS11, consisting of the sensor kinase, hk11, and its cognate response regulator, rr11, in the setting of increased biofilm formation. These effects of cigarette smoke on the pneumococcus may contribute to colonization of the airways by this microbial pathogen.

  7. Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System.

    Science.gov (United States)

    Ganesh, Irisappan; Vidhya, Selvamani; Eom, Gyeong Tae; Hong, Soon Ho

    2017-06-28

    Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.

  8. Chromatographic studies on thermal and radiolytic degradation of n-dodecane-HNO3 two components system and its purification employing silica gel treatment

    International Nuclear Information System (INIS)

    Kadam, Prashant; Kaushik, C.P.; Ozarde, P.D.; Bindu, M.; Tripathi, S.C.; Jambunathan, U.; Pandit, G.G.

    2005-01-01

    This paper describes the studies carried out to examine the extent of degradation of n-dodecane at elevated temperature for different time periods and the effect of gamma radiolysis at different absorbed dose, in n-dodecane- nitric acid two components system. The studies also involved the identification of the degradation products formed during above process using GC-MS. A large number of degradation products were observed. Formation of degradation products increases with absorbed dose and time of heating. Further purification of degraded dodecane was carried out with silica gel crystals as an adsorbent. Treated dodecane samples were subjected to GC/GC-MS analysis, to confirm the extent of removal degradation products. (author)

  9. Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profile

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    Yu Fangyou

    2010-11-01

    Full Text Available Abstract Background Staphylococcus epidermidis has emerged as one of the most important nosocomial pathogens, mainly because of its ability to colonize implanted biomaterials by forming a biofilm. Extensive studies are focused on the molecular mechanisms involved in biofilm formation. The LytSR two-component regulatory system regulates autolysis and biofilm formation in Staphylococcus aureus. However, the role of LytSR played in S. epidermidis remained unknown. Results In the present study, we demonstrated that lytSR knock-out in S. epidermidis did not alter susceptibility to Triton X-100 induced autolysis. Quantitative murein hydrolase assay indicated that disruption of lytSR in S. epidermidis resulted in decreased activities of extracellular murein hydrolases, although zymogram showed no apparent differences in murein hydrolase patterns between S. epidermidis strain 1457 and its lytSR mutant. Compared to the wild-type counterpart, 1457ΔlytSR produced slightly more biofilm, with significantly decreased dead cells inside. Microarray analysis showed that lytSR mutation affected the transcription of 164 genes (123 genes were upregulated and 41 genes were downregulated. Specifically, genes encoding proteins responsible for protein synthesis, energy metabolism were downregulated, while genes involved in amino acid and nucleotide biosynthesis, amino acid transporters were upregulated. Impaired ability to utilize pyruvate and reduced activity of arginine deiminase was observed in 1457ΔlytSR, which is consistent with the microarray data. Conclusions The preliminary results suggest that in S. epidermidis LytSR two-component system regulates extracellular murein hydrolase activity, bacterial cell death and pyruvate utilization. Based on the microarray data, it appears that lytSR inactivation induces a stringent response. In addition, LytSR may indirectly enhance biofilm formation by altering the metabolic status of the bacteria.

  10. Reconfigurable Sensor Monitoring System

    Science.gov (United States)

    Alhorn, Dean C. (Inventor); Dutton, Kenneth R. (Inventor); Howard, David E. (Inventor); Smith, Dennis A. (Inventor)

    2017-01-01

    A reconfigurable sensor monitoring system includes software tunable filters, each of which is programmable to condition one type of analog signal. A processor coupled to the software tunable filters receives each type of analog signal so-conditioned.

  11. The Staphylococcus aureus Two-Component System AgrAC Displays Four Distinct Genomic Arrangements That Delineate Genomic Virulence Factor Signatures

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    Kumari S. Choudhary

    2018-05-01

    Full Text Available Two-component systems (TCSs consist of a histidine kinase and a response regulator. Here, we evaluated the conservation of the AgrAC TCS among 149 completely sequenced Staphylococcus aureus strains. It is composed of four genes: agrBDCA. We found that: (i AgrAC system (agr was found in all but one of the 149 strains, (ii the agr positive strains were further classified into four agr types based on AgrD protein sequences, (iii the four agr types not only specified the chromosomal arrangement of the agr genes but also the sequence divergence of AgrC histidine kinase protein, which confers signal specificity, (iv the sequence divergence was reflected in distinct structural properties especially in the transmembrane region and second extracellular binding domain, and (v there was a strong correlation between the agr type and the virulence genomic profile of the organism. Taken together, these results demonstrate that bioinformatic analysis of the agr locus leads to a classification system that correlates with the presence of virulence factors and protein structural properties.

  12. The Staphylococcus aureus Two-Component System AgrAC Displays Four Distinct Genomic Arrangements That Delineate Genomic Virulence Factor Signatures

    Science.gov (United States)

    Choudhary, Kumari S.; Mih, Nathan; Monk, Jonathan; Kavvas, Erol; Yurkovich, James T.; Sakoulas, George; Palsson, Bernhard O.

    2018-01-01

    Two-component systems (TCSs) consist of a histidine kinase and a response regulator. Here, we evaluated the conservation of the AgrAC TCS among 149 completely sequenced Staphylococcus aureus strains. It is composed of four genes: agrBDCA. We found that: (i) AgrAC system (agr) was found in all but one of the 149 strains, (ii) the agr positive strains were further classified into four agr types based on AgrD protein sequences, (iii) the four agr types not only specified the chromosomal arrangement of the agr genes but also the sequence divergence of AgrC histidine kinase protein, which confers signal specificity, (iv) the sequence divergence was reflected in distinct structural properties especially in the transmembrane region and second extracellular binding domain, and (v) there was a strong correlation between the agr type and the virulence genomic profile of the organism. Taken together, these results demonstrate that bioinformatic analysis of the agr locus leads to a classification system that correlates with the presence of virulence factors and protein structural properties. PMID:29887846

  13. Identification of genes for small non-coding RNAs that belong to the regulon of the two-component regulatory system CiaRH in Streptococcus

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    Hakenbeck Regine

    2010-11-01

    Full Text Available Abstract Background Post-transcriptional regulation by small RNAs (sRNAs in bacteria is now recognized as a wide-spread regulatory mechanism modulating a variety of physiological responses including virulence. In Streptococcus pneumoniae, an important human pathogen, the first sRNAs to be described were found in the regulon of the CiaRH two-component regulatory system. Five of these sRNAs were detected and designated csRNAs for cia-dependent small RNAs. CiaRH pleiotropically affects β-lactam resistance, autolysis, virulence, and competence development by yet to be defined molecular mechanisms. Since CiaRH is highly conserved among streptococci, it is of interest to determine if csRNAs are also included in the CiaRH regulon in this group of organisms consisting of commensal as well as pathogenic species. Knowledge on the participation of csRNAs in CiaRH-dependent regulatory events will be the key to define the physiological role of this important control system. Results Genes for csRNAs were predicted in streptococcal genomes and data base entries other than S. pneumoniae by searching for CiaR-activated promoters located in intergenic regions that are followed by a transcriptional terminator. 61 different candidate genes were obtained specifying csRNAs ranging in size from 51 to 202 nt. Comparing these genes among each other revealed 40 different csRNA types. All streptococcal genomes harbored csRNA genes, their numbers varying between two and six. To validate these predictions, S. mitis, S. oralis, and S. sanguinis were subjected to csRNA-specific northern blot analysis. In addition, a csRNA gene from S. thermophilus plasmid pST0 introduced into S. pneumoniae was also tested. Each of the csRNAs was detected on these blots and showed the anticipated sizes. Thus, the method applied here is able to predict csRNAs with high precision. Conclusions The results of this study strongly suggest that genes for small non-coding RNAs, csRNAs, are part of

  14. Smart Optoelectronic Sensors and Intelligent Sensor Systems

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    Sergey Y. YURISH

    2012-03-01

    Full Text Available Light-to-frequency converters are widely used in various optoelectronic sensor systems. However, a further frequency-to-digital conversion is a bottleneck in such systems due to a broad frequency range of light-to-frequency converters’ outputs. This paper describes an effective OEM design approach, which can be used for smart and intelligent sensor systems design. The design is based on novel, multifunctional integrated circuit of Universal Sensors & Transducers Interface especially designed for such sensor applications. Experimental results have confirmed an efficiency of this approach and high metrological performances.

  15. The Acinetobacter baumannii Two-Component System AdeRS Regulates Genes Required for Multidrug Efflux, Biofilm Formation, and Virulence in a Strain-Specific Manner

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    Grace E. Richmond

    2016-04-01

    Full Text Available The opportunistic pathogen Acinetobacter baumannii is able to persist in the environment and is often multidrug resistant (MDR, causing difficulties in the treatment of infections. Here, we show that the two-component system AdeRS, which regulates the production of the AdeABC multidrug resistance efflux pump, is required for the formation of a protective biofilm in an ex vivo porcine mucosal model, which mimics a natural infection of the human epithelium. Interestingly, deletion of adeB impacted only on the ability of strain AYE to form a biofilm on plastic and only on the virulence of strain Singapore 1 for Galleria mellonella. RNA-Seq revealed that loss of AdeRS or AdeB significantly altered the transcriptional landscape, resulting in the changed expression of many genes, notably those associated with antimicrobial resistance and virulence interactions. For example, A. baumannii lacking AdeRS displayed decreased expression of adeABC, pil genes, com genes, and a pgaC-like gene, whereas loss of AdeB resulted in increased expression of pil and com genes and decreased expression of ferric acinetobactin transport system genes. These data define the scope of AdeRS-mediated regulation, show that changes in the production of AdeABC mediate important phenotypes controlled by AdeRS, and suggest that AdeABC is a viable target for antimicrobial drug and antibiofilm discovery.

  16. Regulation of Inducible Potassium Transporter KdpFABC by the KdpD/KdpE Two-Component System in Mycobacterium smegmatis

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    Jin He

    2017-04-01

    Full Text Available Kdp-ATPase is an inducible high affinity potassium uptake system that is widely distributed in bacteria, and is generally regulated by the KdpD/KdpE two-component system (TCS. In this study, conducted on Mycobacterium smegmatis, the kdpFABC (encoding Kdp-ATPase expression was found to be affected by low concentration of K+, high concentrations of Na+, and/or NH4+ of the medium. The KdpE was found to be a transcriptional regulator that bound to a specific 22-bp sequence in the promoter region of kdpFABC operon to positively regulate kdpFABC expression. The KdpE binding motif was highly conserved in the promoters of kdpFABC among the mycobacterial species. 5′-RACE data indicated a transcriptional start site (TSS of the kdpFABC operon within the coding sequence of MSMEG_5391, which comprised a 120-bp long 5′-UTR and an open reading frame of the 87-bp kdpF gene. The kdpE deletion resulted in altered growth rate under normal and low K+ conditions. Furthermore, under K+ limiting conditions, a single transcript (kdpFABCDE spanning kdpFABC and kdpDE operons was observed. This study provided the first insight into the regulation of kdpFABC operon by the KdpD/KdpE TCS in M. smegmatis.

  17. Triggering sporulation in Bacillus subtilis with artificial two-component systems reveals the importance of proper Spo0A activation dynamics.

    Science.gov (United States)

    Vishnoi, Monika; Narula, Jatin; Devi, Seram Nganbiton; Dao, Hoang-Anh; Igoshin, Oleg A; Fujita, Masaya

    2013-10-01

    Sporulation initiation in Bacillus subtilis is controlled by the phosphorylated form of the master regulator Spo0A which controls transcription of a multitude of sporulation genes. In this study, we investigated the importance of temporal dynamics of phosphorylated Spo0A (Spo0A∼P) accumulation by rewiring the network controlling its phosphorylation. We showed that simultaneous induction of KinC, a kinase that can directly phosphorylate Spo0A, and Spo0A itself from separately controlled inducible promoters can efficiently trigger sporulation even under nutrient rich conditions. However, the sporulation efficiency in this artificial two-component system was significantly impaired when KinC and/or Spo0A induction was too high. Using mathematical modelling, we showed that gradual accumulation of Spo0A∼P is essential for the proper temporal order of the Spo0A regulon expression, and that reduction in sporulation efficiency results from the reversal of that order. These insights led us to identify premature repression of DivIVA as one possible explanation for the adverse effects of accelerated accumulation of Spo0A∼P on sporulation. Moreover, we found that positive feedback resulting from autoregulation of the native spo0A promoter leads to robust control of Spo0A∼P accumulation kinetics. Thus we propose that a major function of the conserved architecture of the sporulation network is controlling Spo0A activation dynamics. © 2013 John Wiley & Sons Ltd.

  18. Mutation of the Streptococcus gordonii Thiol-Disulfide Oxidoreductase SdbA Leads to Enhanced Biofilm Formation Mediated by the CiaRH Two-Component Signaling System.

    Directory of Open Access Journals (Sweden)

    Lauren Davey

    Full Text Available Streptococcus gordonii is a commensal inhabitant of human oral biofilms. Previously, we identified an enzyme called SdbA that played an important role in biofilm formation by S. gordonii. SdbA is thiol-disulfide oxidoreductase that catalyzes disulfide bonds in secreted proteins. Surprisingly, inactivation of SdbA results in enhanced biofilm formation. In this study we investigated the basis for biofilm formation by the ΔsdbA mutant. The results revealed that biofilm formation was mediated by the interaction between the CiaRH and ComDE two-component signalling systems. Although it did not affect biofilm formation by the S. gordonii parent strain, CiaRH was upregulated in the ΔsdbA mutant and it was essential for the enhanced biofilm phenotype. The biofilm phenotype was reversed by inactivation of CiaRH or by the addition of competence stimulating peptide, the production of which is blocked by CiaRH activity. Competition assays showed that the enhanced biofilm phenotype also corresponded to increased oral colonization in mice. Thus, the interaction between SdbA, CiaRH and ComDE affects biofilm formation both in vitro and in vivo.

  19. A two-component regulatory system, pehR-pehS, controls endopolygalacturonase production and virulence in the plant pathogen Erwinia carotovora subsp. carotovora.

    Science.gov (United States)

    Flego, D; Marits, R; Eriksson, A R; Kõiv, V; Karlsson, M B; Heikinheimo, R; Palva, E T

    2000-04-01

    Genes coding for the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the plant cell wall-degrading enzymes, are under the coordinate control of global regulator systems including both positive and negative factors. In addition to this global control, some virulence determinants are subject to specific regulation. We have previously shown that mutations in the pehR locus result in reduced virulence and impaired production of one of these enzymes, an endopolygalacturonase (PehA). In contrast, these pehR strains produce essentially wild-type levels of other extracellular enzymes including pectate lyases and cellulases. In this work, we characterized the pehR locus and showed that the DNA sequence is composed of two genes, designated pehR and pehS, present in an operon. Mutations in either pehR or pehS caused a Peh-negative phenotype and resulted in reduced virulence on tobacco seedlings. Complementation experiments indicated that both genes are required for transcriptional activation of the endopolygalacturonase gene, pehA, as well as restoration of virulence. Structural characterization of the pehR-pehS operon demonstrated that the corresponding polypeptides are highly similar to the two-component transcriptional regulators PhoP-PhoQ of both Escherichia coli and Salmonella typhimurium. Functional similarity of PehR-PehS with PhoP-PhoQ of E. coli and S. typhimurium was demonstrated by genetic complementation.

  20. The Conserved Actinobacterial Two-Component System MtrAB Coordinates Chloramphenicol Production with Sporulation in Streptomyces venezuelae NRRL B-65442

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    Nicolle F. Som

    2017-06-01

    Full Text Available Streptomyces bacteria make numerous secondary metabolites, including half of all known antibiotics. Production of antibiotics is usually coordinated with the onset of sporulation but the cross regulation of these processes is not fully understood. This is important because most Streptomyces antibiotics are produced at low levels or not at all under laboratory conditions and this makes large scale production of these compounds very challenging. Here, we characterize the highly conserved actinobacterial two-component system MtrAB in the model organism Streptomyces venezuelae and provide evidence that it coordinates production of the antibiotic chloramphenicol with sporulation. MtrAB are known to coordinate DNA replication and cell division in Mycobacterium tuberculosis where TB-MtrA is essential for viability but MtrB is dispensable. We deleted mtrB in S. venezuelae and this resulted in a global shift in the metabolome, including constitutive, higher-level production of chloramphenicol. We found that chloramphenicol is detectable in the wild-type strain, but only at very low levels and only after it has sporulated. ChIP-seq showed that MtrA binds upstream of DNA replication and cell division genes and genes required for chloramphenicol production. dnaA, dnaN, oriC, and wblE (whiB1 are DNA binding targets for MtrA in both M. tuberculosis and S. venezuelae. Intriguingly, over-expression of TB-MtrA and gain of function TB- and Sv-MtrA proteins in S. venezuelae also switched on higher-level production of chloramphenicol. Given the conservation of MtrAB, these constructs might be useful tools for manipulating antibiotic production in other filamentous actinomycetes.

  1. A proteomic analysis of the regulon of the NarP two-component regulatory system response regulator in the bovine pathogen Mannheimia haemolytica A1

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    Inamoto Ichiro

    2011-11-01

    Full Text Available Abstract Background The response of the NarQP two-component signal transduction system regulon in response to the presence of nitrate for the bovine pathogen Mannheimia haemolytica A1 was investigated by proteomic analysis. Total proteins from a narP mutant and the parent SH1217 grown with or without NaNO3 supplement were examined by ISO-DALT 2D electrophoresis and liquid chromatography-mass spectrometry. Results Seventeen proteins were differentially expressed in the parent strain SH1217 in response to the addition of NaNO3 to the growth media. These responses were absent in the narP mutant, indicating that the altered production of these proteins is mediated by NarPMh. Interestingly, NarPMh mediated the increased production of some proteins which are not generally associated with nitrate respiration, such as the iron transporters FbpA and YfeA. The increased production of proteins such as superoxide dismutase, SodA, and GAPDH were also observed. The increased production of these iron-regulated proteins by NarPMh is thought to enhance the swift establishment of the nitrate respiration mechanism of M. haemolytica during pathogenesis. Conclusion The data suggested NarPMh acts as an important regulator which regulates the expression of a small set of proteins in response to nitrate availability. This may contribute to the prevalence of M. haemolytica A1 in its host during pathogenesis of BPP, through enhancing the effectiveness of nitrate respiration either directly or indirectly.

  2. Gene expression analysis of two extensively drug-resistant tuberculosis isolates show that two-component response systems enhance drug resistance.

    Science.gov (United States)

    Yu, Guohua; Cui, Zhenling; Sun, Xian; Peng, Jinfu; Jiang, Jun; Wu, Wei; Huang, Wenhua; Chu, Kaili; Zhang, Lu; Ge, Baoxue; Li, Yao

    2015-05-01

    Global analysis of expression profiles using DNA microarrays was performed between a reference strain H37Rv and two clinical extensively drug-resistant isolates in response to three anti-tuberculosis drug exposures (isoniazid, capreomycin, and rifampicin). A deep analysis was then conducted using a combination of genome sequences of the resistant isolates, resistance information, and related public microarray data. Certain known resistance-associated gene sets were significantly overrepresented in upregulated genes in the resistant isolates relative to that observed in H37Rv, which suggested a link between resistance and expression levels of particular genes. In addition, isoniazid and capreomycin response genes, but not rifampicin, either obtained from published works or our data, were highly consistent with the differentially expressed genes of resistant isolates compared to those of H37Rv, indicating a strong association between drug resistance of the isolates and genes differentially regulated by isoniazid and capreomycin exposures. Based on these results, 92 genes of the studied isolates were identified as candidate resistance genes, 10 of which are known resistance-related genes. Regulatory network analysis of candidate resistance genes using published networks and literature mining showed that three two-component regulatory systems and regulator CRP play significant roles in the resistance of the isolates by mediating the production of essential envelope components. Finally, drug sensitivity testing indicated strong correlations between expression levels of these regulatory genes and sensitivity to multiple anti-tuberculosis drugs in Mycobacterium tuberculosis. These findings may provide novel insights into the mechanism underlying the emergence and development of drug resistance in resistant tuberculosis isolates and useful clues for further studies on this issue. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Equation-free analysis of two-component system signalling model reveals the emergence of co-existing phenotypes in the absence of multistationarity.

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    Rebecca B Hoyle

    Full Text Available Phenotypic differences of genetically identical cells under the same environmental conditions have been attributed to the inherent stochasticity of biochemical processes. Various mechanisms have been suggested, including the existence of alternative steady states in regulatory networks that are reached by means of stochastic fluctuations, long transient excursions from a stable state to an unstable excited state, and the switching on and off of a reaction network according to the availability of a constituent chemical species. Here we analyse a detailed stochastic kinetic model of two-component system signalling in bacteria, and show that alternative phenotypes emerge in the absence of these features. We perform a bifurcation analysis of deterministic reaction rate equations derived from the model, and find that they cannot reproduce the whole range of qualitative responses to external signals demonstrated by direct stochastic simulations. In particular, the mixed mode, where stochastic switching and a graded response are seen simultaneously, is absent. However, probabilistic and equation-free analyses of the stochastic model that calculate stationary states for the mean of an ensemble of stochastic trajectories reveal that slow transcription of either response regulator or histidine kinase leads to the coexistence of an approximate basal solution and a graded response that combine to produce the mixed mode, thus establishing its essential stochastic nature. The same techniques also show that stochasticity results in the observation of an all-or-none bistable response over a much wider range of external signals than would be expected on deterministic grounds. Thus we demonstrate the application of numerical equation-free methods to a detailed biochemical reaction network model, and show that it can provide new insight into the role of stochasticity in the emergence of phenotypic diversity.

  4. A survey of HK, HPt, and RR domains and their organization in two-component systems and phosphorelay proteins of organisms with fully sequenced genomes

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    Baldiri Salvado

    2015-08-01

    Full Text Available Two Component Systems and Phosphorelays (TCS/PR are environmental signal transduction cascades in prokaryotes and, less frequently, in eukaryotes. The internal domain organization of proteins and the topology of TCS/PR cascades play an important role in shaping the responses of the circuits. It is thus important to maintain updated censuses of TCS/PR proteins in order to identify the various topologies used by nature and enable a systematic study of the dynamics associated with those topologies. To create such a census, we analyzed the proteomes of 7,609 organisms from all domains of life with fully sequenced and annotated genomes. To begin, we survey each proteome searching for proteins containing domains that are associated with internal signal transmission within TCS/PR: Histidine Kinase (HK, Response Regulator (RR and Histidine Phosphotranfer (HPt domains, and analyze how these domains are arranged in the individual proteins. Then, we find all types of operon organization and calculate how much more likely are proteins that contain TCS/PR domains to be coded by neighboring genes than one would expect from the genome background of each organism. Finally, we analyze if the fusion of domains into single TCS/PR proteins is more frequently observed than one might expect from the background of each proteome. We find 50 alternative ways in which the HK, HPt, and RR domains are observed to organize into single proteins. In prokaryotes, TCS/PR coding genes tend to be clustered in operons. 90% of all proteins identified in this study contain just one of the three domains, while 8% of the remaining proteins combine one copy of an HK, a RR, and/or an HPt domain. In eukaryotes, 25% of all TCS/PR proteins have more than one domain. These results might have implications for how signals are internally transmitted within TCS/PR cascades. These implications could explain the selection of the various designs in alternative circumstances.

  5. The impact of ColRS two-component system and TtgABC efflux pump on phenol tolerance of Pseudomonas putida becomes evident only in growing bacteria

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    Kivisaar Maia

    2010-04-01

    Full Text Available Abstract Background We have recently found that Pseudomonas putida deficient in ColRS two-component system is sensitive to phenol and displays a serious defect on solid glucose medium where subpopulation of bacteria lyses. The latter phenotype is significantly enhanced by the presence of phenol in growth medium. Here, we focused on identification of factors affecting phenol tolerance of the colR-deficient P. putida. Results By using transposon mutagenesis approach we identified a set of phenol-tolerant derivatives of colR-deficient strain. Surprisingly, half of independent phenol tolerant clones possessed miniTn5 insertion in the ttgABC operon. However, though inactivation of TtgABC efflux pump significantly enhanced phenol tolerance, it did not affect phenol-enhanced autolysis of the colR mutant on glucose medium indicating that phenol- and glucose-caused stresses experienced by the colR-deficient P. putida are not coupled. Inactivation of TtgABC pump significantly increased the phenol tolerance of the wild-type P. putida as well. Comparison of phenol tolerance of growing versus starving bacteria revealed that both ColRS and TtgABC systems affect phenol tolerance only under growth conditions and not under starvation. Flow cytometry analysis showed that phenol strongly inhibited cell division and to some extent also caused cell membrane permeabilization to propidium iodide. Single cell analysis of populations of the ttgC- and colRttgC-deficient strains revealed that their membrane permeabilization by phenol resembles that of the wild-type and the colR mutant, respectively. However, cell division of P. putida with inactivated TtgABC pump seemed to be less sensitive to phenol than that of the parental strain. At the same time, cell division appeared to be more inhibited in the colR-mutant strain than in the wild-type P. putida. Conclusions ColRS signal system and TtgABC efflux pump are involved in the phenol tolerance of P. putida. However, as

  6. Role of the two component signal transduction system CpxAR in conferring cefepime and chloramphenicol resistance in Klebsiella pneumoniae NTUH-K2044.

    Directory of Open Access Journals (Sweden)

    Vijaya Bharathi Srinivasan

    Full Text Available BACKGROUND: Klebsiella pneumoniae is a gram-negative, non-motile, facultative anaerobe belonging to the Enterobacteriaceae family of the γ-Proteobacteria class in the phylum Proteobacteria. Multidrug resistant K. pneumoniae have caused major therapeutic problems worldwide due to emergence of extended-spectrum β-lactamase producing strains. Two-component systems serve as a basic stimulus-response coupling mechanism to allow organisms to sense and respond to changes in many different environmental conditions including antibiotic stress. PRINCIPAL FINDINGS: In the present study, we investigated the role of an uncharacterized cpxAR operon in bacterial physiology and antimicrobial resistance by generating isogenic mutant (ΔcpxAR deficient in the CpxA/CpxR component derived from the hyper mucoidal K1 strain K. pneumoniae NTUH-K2044. The behaviour of ΔcpxAR was determined under hostile conditions, reproducing stresses encountered in the gastrointestinal environment and deletion resulted in higher sensitivity to bile, osmotic and acid stresses. The ΔcpxAR was more susceptible to β-lactams and chloramphenicol than the wild-type strain, and complementation restored the altered phenotypes. The relative change in expression of acrB, acrD, eefB efflux genes were decreased in cpxAR mutant as evidenced by qRT-PCR. Comparison of outer membrane protein profiles indicated a conspicuous difference in the knock out background. Gel shift assays demonstrated direct binding of CpxR(KP to promoter region of ompC(KP in a concentration dependent manner. CONCLUSIONS AND SIGNIFICANCE: The Cpx envelope stress response system is known to be activated by alterations in pH, membrane composition and misfolded proteins, and this systematic investigation reveals its direct involvement in conferring antimicrobial resistance against clinically significant antibiotics for the very first time. Overall results displayed in this report reflect the pleiotropic role of the Cpx

  7. Relationship of the CreBC two-component regulatory system and inner membrane protein CreD with swimming motility in Stenotrophomonas maltophilia.

    Directory of Open Access Journals (Sweden)

    Hsin-Hui Huang

    Full Text Available The CreBC two-component system (TCS is a conserved regulatory system found in Escherichia coli, Aeromonas spp., Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. In this study, we determined how CreBC TCS regulates secreted protease activities and swimming motility using creB, creC, and creBC in-frame deletion mutants (KJΔCreB, KJΔCreC, and KJΔBC of S. maltophilia KJ. Compared to wild-type KJ, KJΔCreB had a comparable secreted protease activity; however, the secreted protease activities were obviously reduced in KJΔCreC and KJΔBC, suggesting that CreC works together with another unidentified response regulator (not CreB to regulate secreted protease activity. Single gene inactivation of creB or creC resulted in mutants with an enhanced swimming motility, and this phenotype was exacerbated in a double mutant KJΔBC. To elucidate the underlying mechanism responsible for the ΔcreBC-mediated swimming enhancement, flagella morphology observation, RNA-seq based transcriptome assay, qRT-PCR, and membrane integrity and potential assessment were performed. Flagella morphological observation ruled out the possibility that swimming enhancement was due to altered flagella morphology. CreBC inactivation upregulated the expression of creD and flagella-associated genes encoding the basal body- and motor-associated proteins. Furthermore, KJΔBC had an increased membrane susceptibility to Triton X-100 and CreD upregulation in KJΔBC partially alleviated the compromise of membrane integrity. The impact of creBC TCS on bacterial membrane potential was assessed by carbonyl cyanide m-chlorophenyl hydrazine (CCCP50 concentration at which 50% of bacterial swimming is inhibited. CCCP50 of wild-type KJ increased when creBC was deleted, indicating an association between the higher membrane potential of KJΔBC cells and enhanced motility. Upregulation of the basal body- and motor-associated genes of flagella in KJΔBC cells may explain the increased

  8. The PorX response regulator of the Porphyromonas gingivalis PorXY two-component system does not directly regulate the Type IX secretion genes but binds the PorL subunit.

    Directory of Open Access Journals (Sweden)

    Maxence S Vincent

    2016-08-01

    Full Text Available The Type IX secretion system (T9SS is a versatile multi-protein complex restricted to bacteria of the Bacteriodetes phylum and responsible for the secretion of surface attachment of diverse proteins that participate to S-layer formation, gliding motility or pathogenesis. The T9SS is poorly characterized but a number of proteins involved in the assembly of the secretion apparatus in the oral pathogen Porphyromonas gingivalis have been identified based on genome substractive analyses. Among these proteins, PorY and PorX encode typical two-component system (TCS sensor and CheY-like response regulator respectively. Although the porX and porY genes do not localize at the same genetic locus, it has been proposed that PorXY form a bona fide TCS. Deletion of the porX in P. gingivalis causes a slight decrease of the expression of a number of other T9SS genes, including sov, porT, porP, porK, porL, porM, porN and porY. Here, we show that PorX and the soluble cytoplasmic domain of PorY interact. Using electrophoretic mobility shift, DNA-protein co-purification and heterologous host expression assays, we showed that PorX does not bind and does not directly regulate expression of the T9SS genes. Finally, we show that PorX interacts with the cytoplasmic domain of PorL, a component of the T9SS membrane core complex and propose that the CheY-like PorX protein might be involved in the dynamics of the T9SS.

  9. The PorX Response Regulator of the Porphyromonas gingivalis PorXY Two-Component System Does Not Directly Regulate the Type IX Secretion Genes but Binds the PorL Subunit

    Science.gov (United States)

    Vincent, Maxence S.; Durand, Eric; Cascales, Eric

    2016-01-01

    The Type IX secretion system (T9SS) is a versatile multi-protein complex restricted to bacteria of the Bacteriodetes phylum and responsible for the secretion or cell surface exposition of diverse proteins that participate to S-layer formation, gliding motility or pathogenesis. The T9SS is poorly characterized but a number of proteins involved in the assembly of the secretion apparatus in the oral pathogen Porphyromonas gingivalis have been identified based on genome substractive analyses. Among these proteins, PorY, and PorX encode typical two-component system (TCS) sensor and CheY-like response regulator respectively. Although the porX and porY genes do not localize at the same genetic locus, it has been proposed that PorXY form a bona fide TCS. Deletion of porX in P. gingivalis causes a slight decrease of the expression of a number of other T9SS genes, including sov, porT, porP, porK, porL, porM, porN, and porY. Here, we show that PorX and the soluble cytoplasmic domain of PorY interact. Using electrophoretic mobility shift, DNA-protein co-purification and heterologous host expression assays, we demonstrate that PorX does not bind T9SS gene promoters and does not directly regulate expression of the T9SS genes. Finally, we show that PorX interacts with the cytoplasmic domain of PorL, a component of the T9SS membrane core complex and propose that the CheY-like PorX protein might be involved in the dynamics of the T9SS. PMID:27630829

  10. Two-Component Signaling System VgrRS Directly Senses Extracytoplasmic and Intracellular Iron to Control Bacterial Adaptation under Iron Depleted Stress.

    Directory of Open Access Journals (Sweden)

    Li Wang

    2016-12-01

    Full Text Available Both iron starvation and excess are detrimental to cellular life, especially for animal and plant pathogens since they always live in iron-limited environments produced by host immune responses. However, how organisms sense and respond to iron is incompletely understood. Herein, we reveal that in the phytopathogenic bacterium Xanthomonas campestris pv. campestris, VgrS (also named ColS is a membrane-bound receptor histidine kinase that senses extracytoplasmic iron limitation in the periplasm, while its cognate response regulator, VgrR (ColR, detects intracellular iron excess. Under iron-depleted conditions, dissociation of Fe3+ from the periplasmic sensor region of VgrS activates the VgrS autophosphorylation and subsequent phosphotransfer to VgrR, an OmpR-family transcription factor that regulates bacterial responses to take up iron. VgrR-VgrS regulon and the consensus DNA binding motif of the transcription factor VgrR were dissected by comparative proteomic and ChIP-seq analyses, which revealed that in reacting to iron-depleted environments, VgrR directly or indirectly controls the expressions of hundreds of genes that are involved in various physiological cascades, especially those associated with iron-uptake. Among them, we demonstrated that the phosphorylated VgrR tightly represses the transcription of a special TonB-dependent receptor gene, tdvA. This regulation is a critical prerequisite for efficient iron uptake and bacterial virulence since activation of tdvA transcription is detrimental to these processes. When the intracellular iron accumulates, the VgrR-Fe2+ interaction dissociates not only the binding between VgrR and the tdvA promoter, but also the interaction between VgrR and VgrS. This relieves the repression in tdvA transcription to impede continuous iron uptake and avoids possible toxic effects of excessive iron accumulation. Our results revealed a signaling system that directly senses both extracytoplasmic and intracellular

  11. Sensor system for web inspection

    Science.gov (United States)

    Sleefe, Gerard E.; Rudnick, Thomas J.; Novak, James L.

    2002-01-01

    A system for electrically measuring variations over a flexible web has a capacitive sensor including spaced electrically conductive, transmit and receive electrodes mounted on a flexible substrate. The sensor is held against a flexible web with sufficient force to deflect the path of the web, which moves relative to the sensor.

  12. Ion mobility sensor system

    Science.gov (United States)

    Xu, Jun; Watson, David B.; Whitten, William B.

    2013-01-22

    An ion mobility sensor system including an ion mobility spectrometer and a differential mobility spectrometer coupled to the ion mobility spectrometer. The ion mobility spectrometer has a first chamber having first end and a second end extending along a first direction, and a first electrode system that generates a constant electric field parallel to the first direction. The differential mobility spectrometer includes a second chamber having a third end and a fourth end configured such that a fluid may flow in a second direction from the third end to the fourth end, and a second electrode system that generates an asymmetric electric field within an interior of the second chamber. Additionally, the ion mobility spectrometer and the differential mobility spectrometer form an interface region. Also, the first end and the third end are positioned facing one another so that the constant electric field enters the third end and overlaps the fluid flowing in the second direction.

  13. Two Component Signal Transduction in Desulfovibrio Species

    Energy Technology Data Exchange (ETDEWEB)

    Luning, Eric; Rajeev, Lara; Ray, Jayashree; Mukhopadhyay, Aindrila

    2010-05-17

    The environmentally relevant Desulfovibrio species are sulfate-reducing bacteria that are of interest in the bioremediation of heavy metal contaminated water. Among these, the genome of D. vulgaris Hildenborough encodes a large number of two component systems consisting of 72 putative response regulators (RR) and 64 putative histidinekinases (HK), the majority of which are uncharacterized. We classified the D. vulgaris Hildenborough RRs based on their output domains and compared the distribution of RRs in other sequenced Desulfovibrio species. We have successfully purified most RRs and several HKs as His-tagged proteins. We performed phospho-transfer experiments to verify relationships between cognate pairs of HK and RR, and we have also mapped a few non-cognate HK-RR pairs. Presented here are our discoveries from the Desulfovibrio RR categorization and results from the in vitro studies using purified His tagged D. vulgaris HKs and RRs.

  14. Water-Cut Sensor System

    KAUST Repository

    Karimi, Muhammad Akram; Shamim, Atif; Arsalan, Muhammad

    2018-01-01

    Provided in some embodiments is a method of manufacturing a pipe conformable water-cut sensors system. Provided in some embodiments is method for manufacturing a water-cut sensor system that includes providing a helical T-resonator, a helical ground

  15. ALC Rooftop Sensor System

    Science.gov (United States)

    2017-10-31

    Department of the Army position unless so designated by other authorized documents. Citation of manufacturer’s or trade names does not constitute an... Interior view of the new sensor box ...................................................... 3 Fig. 4 Interior of original sensor box...7 Fig. 10 Interior of fiber patch panel .................................................................. 7 Fig. 11

  16. Irradiance sensors for solar systems

    Energy Technology Data Exchange (ETDEWEB)

    Storch, A.; Schindl, J. [Oesterreichisches Forschungs- und Pruefzentrum Arsenal GesmbH, Vienna (Austria). Business Unit Renewable Energy

    2004-07-01

    The presented project surveyed the quality of irradiance sensors used for applications in solar systems. By analysing an outdoor measurement, the accuracies of ten commercially available irradiance sensors were evaluated, comparing their results to those of a calibrated Kipp and Zonen pyranometer CM21. Furthermore, as a simple method for improving the quality of the results, for each sensor an irradiance-calibration was carried out and examined for its effectiveness. (orig.)

  17. A 3D visualization of the substituent effect : A brief analysis of two components of the operational formula of dual descriptor for open-shell systems.

    Science.gov (United States)

    Martínez-Araya, Jorge I; Yepes, Diana; Jaque, Pablo

    2017-12-27

    Six organometallic compounds coming from a basic Mo-based complex were analyzed from the perspective of the dual descriptor in order to detect subtle influences that a substituent group could exert on the reactive core at a long range. Since the aforementioned complexes are open-shell systems, the used operational formula for the dual descriptor is that one defined for those aforementioned systems, which was then compared with spin density. In addition, dual descriptor was decomposed into two terms, each of which was also applied on every molecular system. The obtained results indicated that components of dual descriptor could become more useful than the operational formula of dual descriptor because differences exerted by the substituents at the para position were better detected by components of dual descriptor rather than the dual descriptor by itself.

  18. An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence

    NARCIS (Netherlands)

    Sturme, M.H.J.; Nakayama, J.; Molenaar, D.; Murakami, Y.; Kunugi, R.; Fujii, T.; Vaughan, E.E.; Kleerebezem, M.; Vos, de W.M.

    2005-01-01

    We have analyzed a locus on the annotated Lactobacillus plantarum WCFS1 genome that showed homology to the staphylococcal agr quorum-sensing system and designated it lam for Lactobacillus agr-like module. Production of the lamBDCA transcript was shown to be growth phase dependent. Analysis of a

  19. The Two-Component System CprRS Senses Cationic Peptides and Triggers Adaptive Resistance in Pseudomonas aeruginosa Independently of ParRS

    DEFF Research Database (Denmark)

    Fernandez, Luca; Jenssen, Håvard; Bains, Manjeet

    2012-01-01

    dependency on the CprRS and ParRS systems in a concentration-dependent manner. It was further demonstrated that, following exposure to inducing antimicrobial peptides, cprRS mutants did not become adaptively resistant to polymyxins as was observed for wild-type cells. Our microarray studies demonstrated...

  20. Monoclonal antibody heterogeneity analysis and deamidation monitoring with high-performance cation-exchange chromatofocusing using simple, two component buffer systems.

    Science.gov (United States)

    Kang, Xuezhen; Kutzko, Joseph P; Hayes, Michael L; Frey, Douglas D

    2013-03-29

    The use of either a polyampholyte buffer or a simple buffer system for the high-performance cation-exchange chromatofocusing of monoclonal antibodies is demonstrated for the case where the pH gradient is produced entirely inside the column and with no external mixing of buffers. The simple buffer system used was composed of two buffering species, one which becomes adsorbed onto the column packing and one which does not adsorb, together with an adsorbed ion that does not participate in acid-base equilibrium. The method which employs the simple buffer system is capable of producing a gradual pH gradient in the neutral to acidic pH range that can be adjusted by proper selection of the starting and ending pH values for the gradient as well as the buffering species concentration, pKa, and molecular size. By using this approach, variants of representative monoclonal antibodies with isoelectric points of 7.0 or less were separated with high resolution so that the approach can serve as a complementary alternative to isoelectric focusing for characterizing a monoclonal antibody based on differences in the isoelectric points of the variants present. Because the simple buffer system used eliminates the use of polyampholytes, the method is suitable for antibody heterogeneity analysis coupled with mass spectrometry. The method can also be used at the preparative scale to collect highly purified isoelectric variants of an antibody for further study. To illustrate this, a single isoelectric point variant of a monoclonal antibody was collected and used for a stability study under forced deamidation conditions. Copyright © 2013 Elsevier B.V. All rights reserved.

  1. Common bus multinode sensor system

    International Nuclear Information System (INIS)

    Kelly, T.F.; Naviasky, E.H.; Evans, W.P.; Jefferies, D.W.; Smith, J.R.

    1988-01-01

    This patent describes a nuclear power plant including a common bus multinode sensor system for sensors in the nuclear power plant, each sensor producing a sensor signal. The system consists of: a power supply providing power; a communication cable coupled to the power supply; plural remote sensor units coupled between the cable and one or more sensors, and comprising: a direct current power supply, connected to the cable and converting the power on the cable into direct current; an analog-to-digital converter connected to the direct current power supply; an oscillator reference; a filter; and an integrated circuit sensor interface connected to the direct current power supply, the analog-to-digital converter, the oscillator crystal and the filter, the interface comprising: a counter receiving a frequency designation word from external to the interface; a phase-frequency comparator connected to the counter; an oscillator connected to the oscillator reference; a timing counter connected to the oscillator, the phase/frequency comparator and the analog-to-digital converter; an analog multiplexer connectable to the sensors and the analog-to-digital converter, and connected to the timing counter; a shift register operatively connected to the timing counter and the analog-to-digital converter; an encoder connected to the shift register and connectable to the filter; and a voltage controlled oscillator connected to the filter and the cable

  2. NSTX High Temperature Sensor Systems

    International Nuclear Information System (INIS)

    McCormack, B.; Kugel, H.W.; Goranson, P.; Kaita, R.

    1999-01-01

    The design of the more than 300 in-vessel sensor systems for the National Spherical Torus Experiment (NSTX) has encountered several challenging fusion reactor diagnostic issues involving high temperatures and space constraints. This has resulted in unique miniature, high temperature in-vessel sensor systems mounted in small spaces behind plasma facing armor tiles, and they are prototypical of possible high power reactor first-wall applications. In the Center Stack, Divertor, Passive Plate, and vessel wall regions, the small magnetic sensors, large magnetic sensors, flux loops, Rogowski Coils, thermocouples, and Langmuir Probes are qualified for 600 degrees C operation. This rating will accommodate both peak rear-face graphite tile temperatures during operations and the 350 degrees C bake-out conditions. Similar sensor systems including flux loops, on other vacuum vessel regions are qualified for 350 degrees C operation. Cabling from the sensors embedded in the graphite tiles follows narrow routes to exit the vessel. The detailed sensor design and installation methods of these diagnostic systems developed for high-powered ST operation are discussed

  3. Wearable Sensor Systems for Infants

    Directory of Open Access Journals (Sweden)

    Zhihua Zhu

    2015-02-01

    Full Text Available Continuous health status monitoring of infants is achieved with the development and fusion of wearable sensing technologies, wireless communication techniques and a low energy-consumption microprocessor with high performance data processing algorithms. As a clinical tool applied in the constant monitoring of physiological parameters of infants, wearable sensor systems for infants are able to transmit the information obtained inside an infant’s body to clinicians or parents. Moreover, such systems with integrated sensors can perceive external threats such as falling or drowning and warn parents immediately. Firstly, the paper reviews some available wearable sensor systems for infants; secondly, we introduce the different modules of the framework in the sensor systems; lastly, the methods and techniques applied in the wearable sensor systems are summarized and discussed. The latest research and achievements have been highlighted in this paper and the meaningful applications in healthcare and behavior analysis are also presented. Moreover, we give a lucid perspective of the development of wearable sensor systems for infants in the future.

  4. Spin-excited oscillations in two-component fermion condensates

    International Nuclear Information System (INIS)

    Maruyama, Tomoyuki; Bertsch, George F.

    2006-01-01

    We investigate collective spin excitations in two-component fermion condensates with special consideration of unequal populations of the two components. The frequencies of monopole and dipole modes are calculated using Thomas-Fermi theory and the scaling approximation. As the fermion-fermion coupling is varied, the system shows various phases of the spin configuration. We demonstrate that spin oscillations have more sensitivity to the spin phase structures than the density oscillations

  5. Characterization of the CrbS/R Two-Component System in Pseudomonas fluorescens Reveals a New Set of Genes under Its Control and a DNA Motif Required for CrbR-Mediated Transcriptional Activation

    Directory of Open Access Journals (Sweden)

    Edgardo Sepulveda

    2017-11-01

    Full Text Available The CrbS/R system is a two-component signal transduction system that regulates acetate utilization in Vibrio cholerae, P. aeruginosa, and P. entomophila. CrbS is a hybrid histidine kinase that belongs to a recently identified family, in which the signaling domain is fused to an SLC5 solute symporter domain through aSTAC domain. Upon activation by CrbS, CrbR activates transcription of the acs gene, which encodes an acetyl-CoA synthase (ACS, and the actP gene, which encodes an acetate/solute symporter. In this work, we characterized the CrbS/R system in Pseudomonas fluorescens SBW25. Through the quantitative proteome analysis of different mutants, we were able to identify a new set of genes under its control, which play an important role during growth on acetate. These results led us to the identification of a conserved DNA motif in the putative promoter region of acetate-utilization genes in the Gammaproteobacteria that is essential for the CrbR-mediated transcriptional activation of genes under acetate-utilizing conditions. Finally, we took advantage of the existence of a second SLC5-containing two-component signal transduction system in P. fluorescens, CbrA/B, to demonstrate that the activation of the response regulator by the histidine kinase is not dependent on substrate transport through the SLC5 domain.

  6. The Ca2+ induced two-component system, CvsSR regulates the Type III secretion system and the extracytoplasmic function sigma-factor AlgU in Pseudomonas syringae pv. tomato DC3000.

    Science.gov (United States)

    Fishman, Maxwell R; Zhang, Johnson; Bronstein, Philip A; Stodghill, Paul; Filiatrault, Melanie J

    2017-12-20

    Two-component systems (TCSs) of bacteria regulate many different aspects of the bacterial life cycle including pathogenesis. Most TCSs remain uncharacterized with no information about the signal(s) or regulatory targets and/or role in bacterial pathogenesis. Here, we characterized a TCS in the plant-pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 ( Pto ) composed of the histidine kinase, CvsS, and the response regulator, CvsR. CvsSR is necessary for virulence of Pto , since ΔcvsS and ΔcvsR strains produced fewer symptoms and demonstrated reduced growth on multiple hosts as compared to WT. We discovered that expression of cvsSR is induced by Ca 2+ concentrations found in leaf apoplastic fluid. Thus, Ca 2+ can be added to the list of signals that promote pathogenesis of Pto during host colonization. Through chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) and global transcriptome analysis (RNA-seq) we discerned the CvsR regulon. CvsR directly activated expression of the type III secretion system regulators, hrpR and hrpS , that regulates Pto virulence in a type III secretion system dependent manner. CvsR also indirectly repressed transcription of the extracytoplasmic sigma factor algU and production of alginate. Phenotypic analysis determined that CvsSR inversely regulated biofilm formation, swarming motility, and cellulose production in a Ca 2+ -dependent manner. Overall, our results show that CvsSR is a key regulatory hub critical for interaction with host plants. Importance Pathogenic bacteria must be able to react and respond to the surrounding environment, make use of available resources, and avert or counter host immune responses. Often, these abilities rely on two-component systems (TCSs) composed of interacting proteins that modulate gene expression. We identified a TCS in the plant-pathogenic bacterium Pseudomonas syringae that responds to the presence of calcium, which is an important signal during the plant

  7. Next generation sensors and systems

    CERN Document Server

    2016-01-01

    Written by experts in their area of research, this book has outlined the current status of the fundamentals and analytical concepts, modelling and design issues, technical details and practical applications of different types of sensors and discussed about the trends of next generation of sensors and systems happening in the area of Sensing technology. This book will be useful as a reference book for engineers and scientist especially the post-graduate students find will this book as reference book for their research on wearable sensors, devices and technologies.  .

  8. Fusion of Images from Dissimilar Sensor Systems

    National Research Council Canada - National Science Library

    Chow, Khin

    2004-01-01

    Different sensors exploit different regions of the electromagnetic spectrum; therefore a multi-sensor image fusion system can take full advantage of the complementary capabilities of individual sensors in the suit...

  9. Two component plasma vortex approach to fusion

    International Nuclear Information System (INIS)

    Ikuta, Kazunari.

    1978-09-01

    Two component operation of the field reversed theta pinch plasma by injection of the energetic ion beam with energy of the order of 1 MeV is considered. A possible trapping scheme of the ion beam in the plasma is discussed in detail. (author)

  10. Battery system with temperature sensors

    Science.gov (United States)

    Wood, Steven J.; Trester, Dale B.

    2012-11-13

    A battery system to monitor temperature includes at least one cell with a temperature sensing device proximate the at least one cell. The battery system also includes a flexible member that holds the temperature sensor proximate to the at least one cell.

  11. Two-component feedback loops and deformed mechanics

    International Nuclear Information System (INIS)

    Tourigny, David S.

    2015-01-01

    It is shown that a general two-component feedback loop can be viewed as a deformed Hamiltonian system. Some of the implications of using ideas from theoretical physics to study biological processes are discussed. - Highlights: • Two-component molecular feedback loops are viewed as q-deformed Hamiltonian systems. • Deformations are reversed using Jackson derivatives to take advantage of working in the Hamiltonian limit. • New results are derived for the particular examples considered. • General deformations are suggested to be associated with a broader class of biological processes

  12. Two component micro injection moulding for moulded interconnect devices

    DEFF Research Database (Denmark)

    Islam, Aminul

    2008-01-01

    Moulded interconnect devices (MIDs) contain huge possibilities for many applications in micro electro-mechanical-systems because of their capability of reducing the number of components, process steps and finally in miniaturization of the product. Among the available MID process chains, two...... component injection moulding is one of the most industrially adaptive processes. However, the use of two component injection moulding for MID fabrication, with circuit patterns in the sub-millimeter range, is still a big challenge at the present state of technology. The scope of the current Ph.D. project...... and a reasonable adhesion between them. • Selective metallization of the two component plastic part (coating one polymer with metal and leaving the other one uncoated) To overcome these two main issues in MID fabrication for micro applications, the current Ph.D. project explores the technical difficulties...

  13. Development and characterization of two-component albedo based neutron individual monitoring system using thermoluminescent detectors; Desenvolvimento e caracterizacao de um sistema de monitoracao individual de neutrons tipo albedo de duas componentes usando detectores termoluminescentes

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Marcelo Marques

    2008-07-01

    A TLD-albedo based two-component neutron individual monitoring system was developed and characterized in this work. The monitor consists of a black plastic holder, an incident neutron boron loaded shield, a moderator polyethylene body (to increase its response), two pairs of TLD-600 and TLD-700 (one pair to each component) and an adjustable belt. This monitoring system was calibrated in thermal neutron fields and in 70 keV, 144 keV, 565 keV, 1.2 MeV and 5 MeV monoenergetic neutron fields. In addition, it was calibrated in {sup 252C}f(D{sub 2}O), {sup 252}Cf, {sup 241}Am-B, {sup 241}Am-Be and {sup 238}Pu-Be source fields. For the latter, the lower detection levels are, respectively, 0.009 mSv, 0.06 mSv, 0.12 mSv, 0.09 mSv and 0.08 mSv. The participation in an international intercomparison sponsored by IAEA with simulated workplace fields validated the system. The monitoring system was successfully characterized in the ISO 21909 standard and in an IRD - the Brazilian Institute for Radioprotection and Dosimetry - technical regulation draft. Nowadays, the neutron individual system is in use by IRD for whole body individual monitoring of five institutions, which comprehend several activities. (author)

  14. Water-Cut Sensor System

    KAUST Repository

    Karimi, Muhammad Akram

    2018-01-11

    Provided in some embodiments is a method of manufacturing a pipe conformable water-cut sensors system. Provided in some embodiments is method for manufacturing a water-cut sensor system that includes providing a helical T-resonator, a helical ground conductor, and a separator at an exterior of a cylindrical pipe. The helical T-resonator including a feed line, and a helical open shunt stub conductively coupled to the feed line. The helical ground conductor including a helical ground plane opposite the helical open shunt stub and a ground ring conductively coupled to the helical ground plane. The feed line overlapping at least a portion of the ground ring, and the separator disposed between the feed line and the portion of the ground ring overlapped by the feed line to electrically isolate the helical T-resonator from the helical ground conductor.

  15. Primordial two-component maximally symmetric inflation

    Science.gov (United States)

    Enqvist, K.; Nanopoulos, D. V.; Quirós, M.; Kounnas, C.

    1985-12-01

    We propose a two-component inflation model, based on maximally symmetric supergravity, where the scales of reheating and the inflation potential at the origin are decoupled. This is possible because of the second-order phase transition from SU(5) to SU(3)×SU(2)×U(1) that takes place when φ≅φcinflation at the global minimum, and leads to a reheating temperature TR≅(1015-1016) GeV. This makes it possible to generate baryon asymmetry in the conventional way without any conflict with experimental data on proton lifetime. The mass of the gravitinos is m3/2≅1012 GeV, thus avoiding the gravitino problem. Monopoles are diluted by residual inflation in the broken phase below the cosmological bounds if φcUSA.

  16. Integrated Sensor Systems for UAS

    Science.gov (United States)

    2008-04-01

    2. Optical particle counter 0.27 5.4 3. Pyranometer 0.17 ɘ.2 4. Temp. & relative humidity 0.05 ɘ.1 5. Data acquisition system 0.15 ɘ.2 6...payload volume showing sensor instrument installation. The insert shows the Manta exterior with the cloud droplet probe and pyranometer mounted on...Instrumentation Above- 2.7 Aethalometer cloud 14 Optical particle counter Up and down pyranometers Condensation particle counter In- 3.7

  17. Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression.

    Science.gov (United States)

    Deng, Ziqing; Shan, Yue; Pan, Qing; Gao, Xiang; Yan, Aixin

    2013-01-01

    The gadE-mdtEF operon encodes a central acid resistance regulator GadE and two multidrug efflux proteins MdtEF. Although transcriptional regulation of gadE in the context of acid resistance under the aerobic growth environment of Escherichia coli has been extensively studied, regulation of the operon under the physiologically relevant environment of anaerobic growth and its effect on the expression of the multidrug efflux proteins MdtEF in the operon has not been disclosed. Our previous study revealed that anaerobic induction of the operon was dependent on ArcA, the response regulator of the ArcBA two-component system, in the M9 glucose minimal medium. However, the detailed regulatory mechanism remains unknown. In this study, we showed that anaerobic activation of mdtEF was driven by the 798 bp unusually long gadE promoter. Deletion of evgA, ydeO, rpoS, and gadX which has been shown to activate the gadE expression during acid stresses under aerobic condition did not have a significant effect on the anaerobic activation of the operon. Rather, anaerobic activation of the operon was largely dependent on the global regulator ArcA and a GTPase MnmE. Under aerobic condition, transcription of gadE was repressed by the global DNA silencer H-NS in M9 minimal medium. Interestingly, under anaerobic condition, while ΔarcA almost completely abolished transcription of gadE-mdtEF, further deletion of hns in ΔarcA mutant restored the transcription of the full-length PgadE-lacZ, and P1- and P3-lacZ fusions, suggesting an antagonistic effect of ArcA on the H-NS mediated repression. Taken together, we conclude that the anaerobic activation of the gadE-mdtEF was primarily mediated by the two-component system ArcBA through antagonizing the H-NS mediated repression.

  18. Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression

    Directory of Open Access Journals (Sweden)

    Ziqing eDeng

    2013-07-01

    Full Text Available The gadE-mdtEF operon encodes a central acid resistance regulator GadE and two multidrug efflux proteins MdtEF. Although transcriptional regulation of gadE in the context of acid resistance under the aerobic growth environment of E. coli has been extensively studied, regulation of the operon under the physiologically relevant environment of anaerobic growth and its effect on the expression of the multidrug efflux proteins MdtEF has not been disclosed. Our previous study revealed that anaerobic induction of the operon was dependent on ArcA, the response regulator of the ArcBA two-component system, in the M9 glucose minimal medium. However, the detailed regulatory mechanism remains unknown. In this study, we showed that anaerobic activation of mdtEF was driven by the 798bp unusually long gadE promoter. Deletion of evgA, ydeO, rpoS, and gadX which has been shown to activate the gadE expression during acid stresses under aerobic condition did not have a significant effect on the anaerobic activation of the operon. Rather, anaerobic activation of the operon was largely dependent on the global regulator ArcA and a GTPase MnmE. Under aerobic condition, transcription of gadE was repressed by the global DNA silencer H-NS in M9 minimal medium. Interestingly, under anaerobic condition, while ΔarcA almost completely abolished transcription of gadE-mdtEF, further deletion of hns in ΔarcA mutant restored the transcription of the full length PgadE-lacZ, and P1- and P3-lacZ fusions, suggesting an antagonistic effect of ArcA on the H-NS mediated repression. Taken together, we conclude that the anaerobic activation of the gadE-mdtEF was primarily mediated by the two-component system ArcBA through antagonizing the H-NS mediated repression.

  19. Screening of the two-component-system histidine kinases of Listeria monocytogenes EGD-e. LiaS is needed for growth under heat, acid, alkali, osmotic, ethanol and oxidative stresses.

    Science.gov (United States)

    Pöntinen, Anna; Lindström, Miia; Skurnik, Mikael; Korkeala, Hannu

    2017-08-01

    To study the role of each two-component system (TCS) histidine kinase (HK) in stress tolerance of Listeria monocytogenes EGD-e, we monitored the growth of individual HK deletion mutant strains under heat (42.5 °C), acid (pH 5.6), alkali (pH 9.4), osmotic (6% NaCl), ethanol (3.5 vol%), and oxidative (5 mM H 2 O 2 ) stresses. The growth of ΔliaS (Δlmo1021) strain was impaired under each stress, with the most notable decrease under heat and osmotic stresses. The ΔvirS (Δlmo1741) strain showed nearly completely restricted growth at high temperature and impaired growth in ethanol. The growth of ΔagrC (Δlmo0050) strain was impaired under osmotic stress and slightly under oxidative stress. We successfully complemented the HK mutations using a novel allelic exchange based approach. This approach avoided the copy-number problems associated with in trans complementation from a plasmid. The mutant phenotypes were restored to the wild-type level in the complemented strains. This study reveals novel knowledge on the HKs needed for growth of L. monocytogenes EGD-e under abovementioned stress conditions, with LiaS playing multiple roles in stress tolerance of L. monocytogenes EGD-e. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. High pressure fiber optic sensor system

    Science.gov (United States)

    Guida, Renato; Xia, Hua; Lee, Boon K; Dekate, Sachin N

    2013-11-26

    The present application provides a fiber optic sensor system. The fiber optic sensor system may include a small diameter bellows, a large diameter bellows, and a fiber optic pressure sensor attached to the small diameter bellows. Contraction of the large diameter bellows under an applied pressure may cause the small diameter bellows to expand such that the fiber optic pressure sensor may measure the applied pressure.

  1. Internetting tactical security sensor systems

    Science.gov (United States)

    Gage, Douglas W.; Bryan, W. D.; Nguyen, Hoa G.

    1998-08-01

    The Multipurpose Surveillance and Security Mission Platform (MSSMP) is a distributed network of remote sensing packages and control stations, designed to provide a rapidly deployable, extended-range surveillance capability for a wide variety of military security operations and other tactical missions. The baseline MSSMP sensor suite consists of a pan/tilt unit with video and FLIR cameras and laser rangefinder. With an additional radio transceiver, MSSMP can also function as a gateway between existing security/surveillance sensor systems such as TASS, TRSS, and IREMBASS, and IP-based networks, to support the timely distribution of both threat detection and threat assessment information. The MSSMP system makes maximum use of Commercial Off The Shelf (COTS) components for sensing, processing, and communications, and of both established and emerging standard communications networking protocols and system integration techniques. Its use of IP-based protocols allows it to freely interoperate with the Internet -- providing geographic transparency, facilitating development, and allowing fully distributed demonstration capability -- and prepares it for integration with the IP-based tactical radio networks that will evolve in the next decade. Unfortunately, the Internet's standard Transport layer protocol, TCP, is poorly matched to the requirements of security sensors and other quasi- autonomous systems in being oriented to conveying a continuous data stream, rather than discrete messages. Also, its canonical 'socket' interface both conceals short losses of communications connectivity and simply gives up and forces the Application layer software to deal with longer losses. For MSSMP, a software applique is being developed that will run on top of User Datagram Protocol (UDP) to provide a reliable message-based Transport service. In addition, a Session layer protocol is being developed to support the effective transfer of control of multiple platforms among multiple control

  2. Geographically distributed environmental sensor system

    Science.gov (United States)

    French, Patrick; Veatch, Brad; O'Connor, Mike

    2006-10-03

    The present invention is directed to a sensor network that includes a number of sensor units and a base unit. The base station operates in a network discovery mode (in which network topology information is collected) in a data polling mode (in which sensed information is collected from selected sensory units). Each of the sensor units can include a number of features, including an anemometer, a rain gauge, a compass, a GPS receiver, a barometric pressure sensor, an air temperature sensor, a humidity sensor, a level, and a radiant temperature sensor.

  3. Aerial measuring system sensor modeling

    International Nuclear Information System (INIS)

    Detwiler, Rebecca

    2002-01-01

    The AMS fixed-wing and rotary-wing systems are critical National Nuclear Security Administration (NNSA) Emergency Response assets. This project is principally focused on the characterization of the sensors utilized with these systems via radiation transport calculations. The Monte Carlo N-Particle code (MCNP) which has been developed at Los Alamos National Laboratory was used to model the detector response of the AMS fixed wing and helicopter systems. To validate the calculations, benchmark measurements were made for simple source-detector configurations. The fixed-wing system is an important tool in response to incidents involving the release of mixed fission products (a commercial power reactor release), the threat or actual explosion of a Radiological Dispersal Device, and the loss or theft of a large industrial source (a radiography source). Calculations modeled the spectral response for the sensors contained, a 3-element NaI detector pod and HpGe detector, in the relevant energy range of 50 keV to 3 MeV. NaI detector responses were simulated for both point and distributed surface sources as a function of gamma energy and flying altitude. For point sources, photo-peak efficiencies were calculated for a zero radial distance and an offset equal to the altitude. For distributed sources approximating infinite plane, gross count efficiencies were calculated and normalized to a uniform surface deposition of 1 C i/m2

  4. Comparison Between a New, Two-component Compression System With Zinc Paste Bandages for Leg Ulcer Healing: A Prospective, Multicenter, Randomized, Controlled Trial Monitoring Sub-bandage Pressures.

    Science.gov (United States)

    Mosti, Giovanni; Crespi, Aldo; Mattaliano, Vincenzo

    2011-05-01

    Compression therapy is standard treatment for venous leg ulcers. The authors prefer multi-layer, multi-component, stiff, high-pressure bandages to treat venous leg ulcers. The Unna boot (UB) is an example of this type of bandage. The aim of this study was to compare the effectiveness and tolerability of UB to a new, two-component bandage. One hundred (100) patients with venous ulcers were randomized into two groups: group A (n = 50) received UB and group B (n = 50) 3M™ Coban™ 2 Layer Compression System (C2L). All patients were followed weekly for 3 months and then monthly until complete healing was achieved. The primary outcomes were: ulcer healing or surface reduction; pain; and exudate control. The secondary outcomes were: ease of application and removal of the bandage, pressure exerted in the supine and standing position after application and before removal, and bandage comfort. C2L was associated with 100% ulcer healing; 47 out of 50 cases healed within the first 3 months after application of the bandage. Compared with the UB, there was no statistically significant difference. In both groups the effect of compression on pain and overall well being was excellent; pain decreased by 50% within 1-2 weeks and remained low throughout the duration of treatment and overall well being improved significantly. There was no significant difference between the two systems concerning level of comfort. C2L proved to be effective in treating venous ulcers due to its stiffness and pressure. Its effectiveness was similar to UB, which is often considered the gold-standard compression device for venous ulcers. This fact, in combination with high tolerability and ease of application and removal, make this new bandage particularly suitable for the treatment of venous leg ulcers. .

  5. Aerial Measuring System Sensor Modeling

    International Nuclear Information System (INIS)

    Detwiler, R.S.

    2002-01-01

    This project deals with the modeling the Aerial Measuring System (AMS) fixed-wing and rotary-wing sensor systems, which are critical U.S. Department of Energy's National Nuclear Security Administration (NNSA) Consequence Management assets. The fixed-wing system is critical in detecting lost or stolen radiography or medical sources, or mixed fission products as from a commercial power plant release at high flying altitudes. The helicopter is typically used at lower altitudes to determine ground contamination, such as in measuring americium from a plutonium ground dispersal during a cleanup. Since the sensitivity of these instruments as a function of altitude is crucial in estimating detection limits of various ground contaminations and necessary count times, a characterization of their sensitivity as a function of altitude and energy is needed. Experimental data at altitude as well as laboratory benchmarks is important to insure that the strong effects of air attenuation are modeled correctly. The modeling presented here is the first attempt at such a characterization of the equipment for flying altitudes. The sodium iodide (NaI) sensors utilized with these systems were characterized using the Monte Carlo N-Particle code (MCNP) developed at Los Alamos National Laboratory. For the fixed wing system, calculations modeled the spectral response for the 3-element NaI detector pod and High-Purity Germanium (HPGe) detector, in the relevant energy range of 50 keV to 3 MeV. NaI detector responses were simulated for both point and distributed surface sources as a function of gamma energy and flying altitude. For point sources, photopeak efficiencies were calculated for a zero radial distance and an offset equal to the altitude. For distributed sources approximating an infinite plane, gross count efficiencies were calculated and normalized to a uniform surface deposition of 1 microCi/m 2 . The helicopter calculations modeled the transport of americium-241 ( 241 Am) as this is

  6. Optimal sensor configuration for complex systems

    DEFF Research Database (Denmark)

    Sadegh, Payman; Spall, J. C.

    1998-01-01

    . The procedure for sensor configuration is based on the simultaneous perturbation stochastic approximation (SPSA) algorithm. SPSA avoids the need for detailed modeling of the sensor response by simply relying on the observed responses obtained by limited experimentation with test sensor configurations. We......The paper considers the problem of sensor configuration for complex systems with the aim of maximizing the useful information about certain quantities of interest. Our approach involves: 1) definition of an appropriate optimality criterion or performance measure; and 2) description of an efficient...... and practical algorithm for achieving the optimality objective. The criterion for optimal sensor configuration is based on maximizing the overall sensor response while minimizing the correlation among the sensor outputs, so as to minimize the redundant information being provided by the multiple sensors...

  7. Optimal sensor configuration for complex systems

    DEFF Research Database (Denmark)

    Sadegh, Payman; Spall, J. C.

    1998-01-01

    configuration is based on maximizing the overall sensor response while minimizing the correlation among the sensor outputs. The procedure for sensor configuration is based on simultaneous perturbation stochastic approximation (SPSA). SPSA avoids the need for detailed modeling of the sensor response by simply......Considers the problem of sensor configuration for complex systems. Our approach involves definition of an appropriate optimality criterion or performance measure, and description of an efficient and practical algorithm for achieving the optimality objective. The criterion for optimal sensor...... relying on observed responses as obtained by limited experimentation with test sensor configurations. We illustrate the approach with the optimal placement of acoustic sensors for signal detection in structures. This includes both a computer simulation study for an aluminum plate, and real...

  8. Robust Solar Position Sensor for Tracking Systems

    DEFF Research Database (Denmark)

    Ritchie, Ewen; Argeseanu, Alin; Leban, Krisztina Monika

    2009-01-01

    The paper proposes a new solar position sensor used in tracking system control. The main advantages of the new solution are the robustness and the economical aspect. Positioning accuracy of the tracking system that uses the new sensor is better than 1°. The new sensor uses the ancient principle...... of the solar clock. The sensitive elements are eight ordinary photo-resistors. It is important to note that all the sensors are not selected simultaneously. It is not necessary for sensor operating characteristics to be quasi-identical because the sensor principle is based on extreme operating duty measurement...... (bright or dark). In addition, the proposed solar sensor significantly simplifies the operation of the tracking control device....

  9. Distributed sensor coordination for advanced energy systems

    Energy Technology Data Exchange (ETDEWEB)

    Tumer, Kagan [Oregon State Univ., Corvallis, OR (United States). School of Mechanical, Industrial and Manufacturing Engineering

    2015-03-12

    Motivation: The ability to collect key system level information is critical to the safe, efficient and reliable operation of advanced power systems. Recent advances in sensor technology have enabled some level of decision making directly at the sensor level. However, coordinating large numbers of sensors, particularly heterogeneous sensors, to achieve system level objectives such as predicting plant efficiency, reducing downtime or predicting outages requires sophisticated coordination algorithms. Indeed, a critical issue in such systems is how to ensure the interaction of a large number of heterogenous system components do not interfere with one another and lead to undesirable behavior. Objectives and Contributions: The long-term objective of this work is to provide sensor deployment, coordination and networking algorithms for large numbers of sensors to ensure the safe, reliable, and robust operation of advanced energy systems. Our two specific objectives are to: 1. Derive sensor performance metrics for heterogeneous sensor networks. 2. Demonstrate effectiveness, scalability and reconfigurability of heterogeneous sensor network in advanced power systems. The key technical contribution of this work is to push the coordination step to the design of the objective functions of the sensors, allowing networks of heterogeneous sensors to be controlled. By ensuring that the control and coordination is not specific to particular sensor hardware, this approach enables the design and operation of large heterogeneous sensor networks. In addition to the coordination coordination mechanism, this approach allows the system to be reconfigured in response to changing needs (e.g., sudden external events requiring new responses) or changing sensor network characteristics (e.g., sudden changes to plant condition). Impact: The impact of this work extends to a large class of problems relevant to the National Energy Technology Laboratory including sensor placement, heterogeneous sensor

  10. Automatic Water Sensor Window Opening System

    KAUST Repository

    Percher, Michael

    2013-01-01

    A system can automatically open at least one window of a vehicle when the vehicle is being submerged in water. The system can include a water collector and a water sensor, and when the water sensor detects water in the water collector, at least one window of the vehicle opens.

  11. Automatic Water Sensor Window Opening System

    KAUST Repository

    Percher, Michael

    2013-12-05

    A system can automatically open at least one window of a vehicle when the vehicle is being submerged in water. The system can include a water collector and a water sensor, and when the water sensor detects water in the water collector, at least one window of the vehicle opens.

  12. Third-generation imaging sensor system concepts

    Science.gov (United States)

    Reago, Donald A.; Horn, Stuart B.; Campbell, James, Jr.; Vollmerhausen, Richard H.

    1999-07-01

    Second generation forward looking infrared sensors, based on either parallel scanning, long wave (8 - 12 um) time delay and integration HgCdTe detectors or mid wave (3 - 5 um), medium format staring (640 X 480 pixels) InSb detectors, are being fielded. The science and technology community is now turning its attention toward the definition of a future third generation of FLIR sensors, based on emerging research and development efforts. Modeled third generation sensor performance demonstrates a significant improvement in performance over second generation, resulting in enhanced lethality and survivability on the future battlefield. In this paper we present the current thinking on what third generation sensors systems will be and the resulting requirements for third generation focal plane array detectors. Three classes of sensors have been identified. The high performance sensor will contain a megapixel or larger array with at least two colors. Higher operating temperatures will also be the goal here so that power and weight can be reduced. A high performance uncooled sensor is also envisioned that will perform somewhere between first and second generation cooled detectors, but at significantly lower cost, weight, and power. The final third generation sensor is a very low cost micro sensor. This sensor can open up a whole new IR market because of its small size, weight, and cost. Future unattended throwaway sensors, micro UAVs, and helmet mounted IR cameras will be the result of this new class.

  13. Error propagation analysis for a sensor system

    International Nuclear Information System (INIS)

    Yeater, M.L.; Hockenbury, R.W.; Hawkins, J.; Wilkinson, J.

    1976-01-01

    As part of a program to develop reliability methods for operational use with reactor sensors and protective systems, error propagation analyses are being made for each model. An example is a sensor system computer simulation model, in which the sensor system signature is convoluted with a reactor signature to show the effect of each in revealing or obscuring information contained in the other. The error propagation analysis models the system and signature uncertainties and sensitivities, whereas the simulation models the signatures and by extensive repetitions reveals the effect of errors in various reactor input or sensor response data. In the approach for the example presented, the errors accumulated by the signature (set of ''noise'' frequencies) are successively calculated as it is propagated stepwise through a system comprised of sensor and signal processing components. Additional modeling steps include a Fourier transform calculation to produce the usual power spectral density representation of the product signature, and some form of pattern recognition algorithm

  14. Proximity sensor system development. CRADA final report

    International Nuclear Information System (INIS)

    Haley, D.C.; Pigoski, T.M.

    1998-01-01

    Lockheed Martin Energy Research Corporation (LMERC) and Merritt Systems, Inc. (MSI) entered into a Cooperative Research and Development Agreement (CRADA) for the development and demonstration of a compact, modular proximity sensing system suitable for application to a wide class of manipulator systems operated in support of environmental restoration and waste management activities. In teleoperated modes, proximity sensing provides the manipulator operator continuous information regarding the proximity of the manipulator to objects in the workspace. In teleoperated and robotic modes, proximity sensing provides added safety through the implementation of active whole arm collision avoidance capabilities. Oak Ridge National Laboratory (ORNL), managed by LMERC for the United States Department of Energy (DOE), has developed an application specific integrated circuit (ASIC) design for the electronics required to support a modular whole arm proximity sensing system based on the use of capacitive sensors developed at Sandia National Laboratories. The use of ASIC technology greatly reduces the size of the electronics required to support the selected sensor types allowing deployment of many small sensor nodes over a large area of the manipulator surface to provide maximum sensor coverage. The ASIC design also provides a communication interface to support sensor commands from and sensor data transmission to a distributed processing system which allows modular implementation and operation of the sensor system. MSI is a commercial small business specializing in proximity sensing systems based upon infrared and acoustic sensors

  15. Proximity sensor system development. CRADA final report

    Energy Technology Data Exchange (ETDEWEB)

    Haley, D.C. [Oak Ridge National Lab., TN (United States); Pigoski, T.M. [Merrit Systems, Inc. (United States)

    1998-01-01

    Lockheed Martin Energy Research Corporation (LMERC) and Merritt Systems, Inc. (MSI) entered into a Cooperative Research and Development Agreement (CRADA) for the development and demonstration of a compact, modular proximity sensing system suitable for application to a wide class of manipulator systems operated in support of environmental restoration and waste management activities. In teleoperated modes, proximity sensing provides the manipulator operator continuous information regarding the proximity of the manipulator to objects in the workspace. In teleoperated and robotic modes, proximity sensing provides added safety through the implementation of active whole arm collision avoidance capabilities. Oak Ridge National Laboratory (ORNL), managed by LMERC for the United States Department of Energy (DOE), has developed an application specific integrated circuit (ASIC) design for the electronics required to support a modular whole arm proximity sensing system based on the use of capacitive sensors developed at Sandia National Laboratories. The use of ASIC technology greatly reduces the size of the electronics required to support the selected sensor types allowing deployment of many small sensor nodes over a large area of the manipulator surface to provide maximum sensor coverage. The ASIC design also provides a communication interface to support sensor commands from and sensor data transmission to a distributed processing system which allows modular implementation and operation of the sensor system. MSI is a commercial small business specializing in proximity sensing systems based upon infrared and acoustic sensors.

  16. Active Sensor Configuration Validation for Refrigeration Systems

    DEFF Research Database (Denmark)

    Hovgaard, Tobias Gybel; Blanke, Mogens; Niemann, Hans Henrik

    2010-01-01

    -diagnosis methods falling short on this problem, this paper suggests an active diagnosis procedure to isolate sensor faults at the commissioning stage, before normal operation has started. Using statistical methods, residuals are evaluated versus multiple hypothesis models in a minimization process to uniquely......Major faults in the commissioning phase of refrigeration systems are caused by defects related to sensors. With a number of similar sensors available that do not differ by type but only by spatial location in the plant, interchange of sensors is a common defect. With sensors being used quite...... differently by the control system, fault-finding is difficult in practice and defects are regularly causing commissioning delays at considerable expense. Validation and handling of faults in the sensor configuration are therefore essential to cut costs during commissioning. With passive fault...

  17. ENERGY EFFICIENT TRACKING SYSTEM USING WIRELESS SENSORS

    OpenAIRE

    Thankaselvi Kumaresan; Sheryl Mathias; Digja Khanvilkar; Prof. Smita Dange

    2014-01-01

    One of the most important applications of wireless sensor networks (WSNs) is surveillance system, which is used to track moving targets. WSN is composed of a large number of low cost sensors which operate on the power derived from batteries. Energy efficiency is an important issue in WSN, which determines the network lifetime. Due to the need for continuous monitoring with 100% efficiency, keeping all the sensor nodes active permanently leads to fast draining of batteries. Hen...

  18. Sensor-guided threat countermeasure system

    Science.gov (United States)

    Stuart, Brent C.; Hackel, Lloyd A.; Hermann, Mark R.; Armstrong, James P.

    2012-12-25

    A countermeasure system for use by a target to protect against an incoming sensor-guided threat. The system includes a laser system for producing a broadband beam and means for directing the broadband beam from the target to the threat. The countermeasure system comprises the steps of producing a broadband beam and directing the broad band beam from the target to blind or confuse the incoming sensor-guided threat.

  19. Battery management system with distributed wireless sensors

    Science.gov (United States)

    Farmer, Joseph C.; Bandhauer, Todd M.

    2016-02-23

    A system for monitoring parameters of an energy storage system having a multiplicity of individual energy storage cells. A radio frequency identification and sensor unit is connected to each of the individual energy storage cells. The radio frequency identification and sensor unit operates to sense the parameter of each individual energy storage cell and provides radio frequency transmission of the parameters of each individual energy storage cell. A management system monitors the radio frequency transmissions from the radio frequency identification and sensor units for monitoring the parameters of the energy storage system.

  20. Optical seismic sensor systems and methods

    Science.gov (United States)

    Beal, A. Craig; Cummings, Malcolm E.; Zavriyev, Anton; Christensen, Caleb A.; Lee, Keun

    2015-12-08

    Disclosed is an optical seismic sensor system for measuring seismic events in a geological formation, including a surface unit for generating and processing an optical signal, and a sensor device optically connected to the surface unit for receiving the optical signal over an optical conduit. The sensor device includes at least one sensor head for sensing a seismic disturbance from at least one direction during a deployment of the sensor device within a borehole of the geological formation. The sensor head includes a frame and a reference mass attached to the frame via at least one flexure, such that movement of the reference mass relative to the frame is constrained to a single predetermined path.

  1. Vibration welding system with thin film sensor

    Science.gov (United States)

    Cai, Wayne W; Abell, Jeffrey A; Li, Xiaochun; Choi, Hongseok; Zhao, Jingzhou

    2014-03-18

    A vibration welding system includes an anvil, a welding horn, a thin film sensor, and a process controller. The anvil and horn include working surfaces that contact a work piece during the welding process. The sensor measures a control value at the working surface. The measured control value is transmitted to the controller, which controls the system in part using the measured control value. The thin film sensor may include a plurality of thermopiles and thermocouples which collectively measure temperature and heat flux at the working surface. A method includes providing a welder device with a slot adjacent to a working surface of the welder device, inserting the thin film sensor into the slot, and using the sensor to measure a control value at the working surface. A process controller then controls the vibration welding system in part using the measured control value.

  2. Molecular Cloning and Bioinformatics Analysis of Two-component Regulation System PhoR/PhoB fromVibrio alginolyticus HY9901%溶藻弧菌双组分调控系统PhoR/PhoB的基因克隆及生物信息学分析

    Institute of Scientific and Technical Information of China (English)

    张燕飞; 庞欢瑛; 吴灶和; 简纪常; 鲁义善

    2014-01-01

    Two-component regulatory system plays an important role in the growth and virulence of bacteria. In this study, the phosphate regulatory sensor protein (PhoR) gene and phosphate transcriptional regulatory protein(PhoB) gene ofVibrio alginolyticus strain HY9901 were cloned. Sequence analysis revealed that the length ofphoR gene was 1 299 bp, encoding a putative protein of 432 amino acids. The length ofphoB gene was 690 bp and encodes a putative protein of 299 amino acids. A phylogenetic tree was constructed by MEGA 5.0 software, indicating that the PhoR and PhoB ofV. alginolyticus showed high genetic relationship withVibrioparahaemolyticusand Vibrio harveyi. By SWISS-MODEL work-space, the three-dimensional structures of HATPase_c and REC conserved domain in the PhoR and PhoB were determined, showing that HATPase_c and REC domain contained an ATP binding site and four aspartic acid active sites, respectively, playing critical roles in regulation of growing and virulence. These results would provide a basis for the further studies on the biological function and drug development of PhoR / PhoB.%双组分调控系统(Two-component Regulatory System)在致病菌的生长及毒力调控中起重要作用。克隆溶藻弧菌(Vibrio alginolyticus)HY9901株组氨酸激酶PhoR和反应调控因子PhoB的全长基因,并对其进行生物信息学分析。序列分析结果显示,phoR(GenBank登录号:KJ958404)全长1299 bp,共编码432个氨基酸;phoB (GenBank登录号:KJ863646)全长690 bp,编码229个氨基酸。构建PhoR/PhoB的系统进化树,结果显示,溶藻弧菌PhoR/PhoB与副溶血弧菌(Vibrio parahaemolyticus)、哈氏弧菌(Vibrio harveyi)有较近亲缘关系。利用SWISS-MODEL软件,对PhoR/PhoB中两个相对保守的功能域HATPase_c和REC进行同源建模,发现HATPase_c具有1个ATP结合位点,REC具有4个天冬氨酸活性位点。

  3. Sensor system for fuel transport vehicle

    Science.gov (United States)

    Earl, Dennis Duncan; McIntyre, Timothy J.; West, David L.

    2016-03-22

    An exemplary sensor system for a fuel transport vehicle can comprise a fuel marker sensor positioned between a fuel storage chamber of the vehicle and an access valve for the fuel storage chamber of the vehicle. The fuel marker sensor can be configured to measure one or more characteristics of one or more fuel markers present in the fuel adjacent the sensor, such as when the marked fuel is unloaded at a retail station. The one or more characteristics can comprise concentration and/or identity of the one or more fuel markers in the fuel. Based on the measured characteristics of the one or more fuel markers, the sensor system can identify the fuel and/or can determine whether the fuel has been adulterated after the marked fuel was last measured, such as when the marked fuel was loaded into the vehicle.

  4. Sensor Arrays and Electronic Tongue Systems

    Directory of Open Access Journals (Sweden)

    Manel del Valle

    2012-01-01

    Full Text Available This paper describes recent work performed with electronic tongue systems utilizing electrochemical sensors. The electronic tongues concept is a new trend in sensors that uses arrays of sensors together with chemometric tools to unravel the complex information generated. Initial contributions and also the most used variant employ conventional ion selective electrodes, in which it is named potentiometric electronic tongue. The second important variant is the one that employs voltammetry for its operation. As chemometric processing tool, the use of artificial neural networks as the preferred data processing variant will be described. The use of the sensor arrays inserted in flow injection or sequential injection systems will exemplify attempts made to automate the operation of electronic tongues. Significant use of biosensors, mainly enzyme-based, to form what is already named bioelectronic tongue will be also presented. Application examples will be illustrated with selected study cases from the Sensors and Biosensors Group at the Autonomous University of Barcelona.

  5. Sensor Technologies for Intelligent Transportation Systems.

    Science.gov (United States)

    Guerrero-Ibáñez, Juan; Zeadally, Sherali; Contreras-Castillo, Juan

    2018-04-16

    Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS) and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment.

  6. Sensor Technologies for Intelligent Transportation Systems

    Science.gov (United States)

    Guerrero-Ibáñez, Juan; Zeadally, Sherali

    2018-01-01

    Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS) and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment. PMID:29659524

  7. Sensor Technologies for Intelligent Transportation Systems

    Directory of Open Access Journals (Sweden)

    Juan Guerrero-Ibáñez

    2018-04-01

    Full Text Available Modern society faces serious problems with transportation systems, including but not limited to traffic congestion, safety, and pollution. Information communication technologies have gained increasing attention and importance in modern transportation systems. Automotive manufacturers are developing in-vehicle sensors and their applications in different areas including safety, traffic management, and infotainment. Government institutions are implementing roadside infrastructures such as cameras and sensors to collect data about environmental and traffic conditions. By seamlessly integrating vehicles and sensing devices, their sensing and communication capabilities can be leveraged to achieve smart and intelligent transportation systems. We discuss how sensor technology can be integrated with the transportation infrastructure to achieve a sustainable Intelligent Transportation System (ITS and how safety, traffic control and infotainment applications can benefit from multiple sensors deployed in different elements of an ITS. Finally, we discuss some of the challenges that need to be addressed to enable a fully operational and cooperative ITS environment.

  8. Vertebrate gravity sensors as dynamic systems

    Science.gov (United States)

    Ross, M. D.

    1985-01-01

    This paper considers verterbrate gravity receptors as dynamic sensors. That is, it is hypothesized that gravity is a constant force to which an acceleration-sensing system would readily adapt. Premises are considered in light of the presence of kinocilia on hair cells of vertebrate gravity sensors; differences in loading of the sensors among species; and of possible reduction in loading by inclusion of much organic material in otoconia. Moreover, organic-inorganic interfaces may confer a piezoelectric property upon otoconia, which increase the sensitivity of the sensory system to small accelerations. Comparisons with man-made accelerometers are briefly taken up.

  9. Optical fiber sensors: Systems and applications. Volume 2

    Science.gov (United States)

    Culshaw, Brian; Dakin, John

    State-of-the-art fiber-optic (FO) sensors and their applications are described in chapters contributed by leading experts. Consideration is given to interferometers, FO gyros, intensity- and wavelength-based sensors and optical actuators, Si in FO sensors, point-sensor multiplexing principles, and distributed FO sensor systems. Also examined are chemical, biochemical, and medical sensors; physical and chemical sensors for process control; FO-sensor applications in the marine and aerospace industries; FO-sensor monitoring systems for security and safety, structural integrity, NDE, and the electric-power industry; and the market situation for FO-sensor technology. Diagrams, drawings, graphs, and photographs are provided.

  10. Toward Sensor-Based Context Aware Systems

    Directory of Open Access Journals (Sweden)

    Kouhei Takada

    2012-01-01

    Full Text Available This paper proposes a methodology for sensor data interpretation that can combine sensor outputs with contexts represented as sets of annotated business rules. Sensor readings are interpreted to generate events labeled with the appropriate type and level of uncertainty. Then, the appropriate context is selected. Reconciliation of different uncertainty types is achieved by a simple technique that moves uncertainty from events to business rules by generating combs of standard Boolean predicates. Finally, context rules are evaluated together with the events to take a decision. The feasibility of our idea is demonstrated via a case study where a context-reasoning engine has been connected to simulated heartbeat sensors using prerecorded experimental data. We use sensor outputs to identify the proper context of operation of a system and trigger decision-making based on context information.

  11. Smart Sensor Network System For Environment Monitoring

    Directory of Open Access Journals (Sweden)

    Javed Ali Baloch

    2012-07-01

    Full Text Available SSN (Smart Sensor Network systems could be used to monitor buildings with modern infrastructure, plant sites with chemical pollution, horticulture, natural habitat, wastewater management and modern transport system. To sense attributes of phenomena and make decisions on the basis of the sensed value is the primary goal of such systems. In this paper a Smart Spatially aware sensor system is presented. A smart system, which could continuously monitor the network to observe the functionality and trigger, alerts to the base station if a change in the system occurs and provide feedback periodically, on demand or even continuously depending on the nature of the application. The results of the simulation trials presented in this paper exhibit the performance of a Smart Spatially Aware Sensor Networks.

  12. Integrated tunneling sensor for nanoelectromechanical systems

    DEFF Research Database (Denmark)

    Sadewasser, S.; Abadal, G.; Barniol, N.

    2006-01-01

    Transducers based on quantum mechanical tunneling provide an extremely sensitive sensor principle, especially for nanoelectromechanical systems. For proper operation a gap between the electrodes of below 1 nm is essential, requiring the use of structures with a mobile electrode. At such small...... distances, attractive van der Waals and capillary forces become sizable, possibly resulting in snap-in of the electrodes. The authors present a comprehensive analysis and evaluation of the interplay between the involved forces and identify requirements for the design of tunneling sensors. Based...... on this analysis, a tunneling sensor is fabricated by Si micromachining technology and its proper operation is demonstrated. (c) 2006 American Institute of Physics....

  13. Distributed Sensor Coordination for Advanced Energy Systems

    Energy Technology Data Exchange (ETDEWEB)

    Tumer, Kagan [Oregon State Univ., Corvallis, OR (United States)

    2013-07-31

    The ability to collect key system level information is critical to the safe, efficient and reliable operation of advanced energy systems. With recent advances in sensor development, it is now possible to push some level of decision making directly to computationally sophisticated sensors, rather than wait for data to arrive to a massive centralized location before a decision is made. This type of approach relies on networked sensors (called “agents” from here on) to actively collect and process data, and provide key control decisions to significantly improve both the quality/relevance of the collected data and the associating decision making. The technological bottlenecks for such sensor networks stem from a lack of mathematics and algorithms to manage the systems, rather than difficulties associated with building and deploying them. Indeed, traditional sensor coordination strategies do not provide adequate solutions for this problem. Passive data collection methods (e.g., large sensor webs) can scale to large systems, but are generally not suited to highly dynamic environments, such as advanced energy systems, where crucial decisions may need to be reached quickly and locally. Approaches based on local decisions on the other hand cannot guarantee that each agent performing its task (maximize an agent objective) will lead to good network wide solution (maximize a network objective) without invoking cumbersome coordination routines. There is currently a lack of algorithms that will enable self-organization and blend the efficiency of local decision making with the system level guarantees of global decision making, particularly when the systems operate in dynamic and stochastic environments. In this work we addressed this critical gap and provided a comprehensive solution to the problem of sensor coordination to ensure the safe, reliable, and robust operation of advanced energy systems. The differentiating aspect of the proposed work is in shifting the focus

  14. Integrating soft sensor systems using conductive thread

    Science.gov (United States)

    Teng, Lijun; Jeronimo, Karina; Wei, Tianqi; Nemitz, Markus P.; Lyu, Geng; Stokes, Adam A.

    2018-05-01

    We are part of a growing community of researchers who are developing a new class of soft machines. By using mechanically soft materials (MPa modulus) we can design systems which overcome the bulk-mechanical mismatches between soft biological systems and hard engineered components. To develop fully integrated soft machines—which include power, communications, and control sub-systems—the research community requires methods for interconnecting between soft and hard electronics. Sensors based upon eutectic gallium alloys in microfluidic channels can be used to measure normal and strain forces, but integrating these sensors into systems of heterogeneous Young’s modulus is difficult due the complexity of finding a material which is electrically conductive, mechanically flexible, and stable over prolonged periods of time. Many existing gallium-based liquid alloy sensors are not mechanically or electrically robust, and have poor stability over time. We present the design and fabrication of a high-resolution pressure-sensor soft system that can transduce normal force into a digital output. In this soft system, which is built on a monolithic silicone substrate, a galinstan-based microfluidic pressure sensor is integrated with a flexible printed circuit board. We used conductive thread as the interconnect and found that this method alleviates problems arising due to the mechanical mismatch between conventional metal wires and soft or liquid materials. Conductive thread is low-cost, it is readily wetted by the liquid metal, it produces little bending moment into the microfluidic channel, and it can be connected directly onto the copper bond-pads of the flexible printed circuit board. We built a bridge-system to provide stable readings from the galinstan pressure sensor. This system gives linear measurement results between 500-3500 Pa of applied pressure. We anticipate that integrated systems of this type will find utility in soft-robotic systems as used for wearable

  15. Micro optical sensor systems for sunsensing applications

    Science.gov (United States)

    Leijtens, Johan; de Boom, Kees

    2017-11-01

    Optimum application of micro system technologies allows building small sensor systems that will alter procurement strategies for spacecraft manufacturers. One example is the decreased size and cost for state of the art sunsensors. Integrated sensor systems are being designed which, through use of microsystem technology, are an order of magnitutde smaller than most current sunsensors and which hold due to the large reproducibility through batch manufacturing the promise of drastic price reduction. If the Commercial Of The Shelf (COTS) approach is adopted by satellite manufacturers, this will drastically decrease mass and cost budgets associated with sunsensing applications.

  16. Passive sensor systems for nuclear material monitoring

    International Nuclear Information System (INIS)

    Simpson, M.L.; Boatner, L.A.; Holcomb, D.E.; McElhaney, S.A.; Mihalczo, J.T.; Muhs, J.D.; Roberts, M.R.; Hill, N.W.

    1993-01-01

    Passive fiber optic sensor systems capable of confirming the presence of special nuclear materials in storage or process facilities are being developed at Oak Ridge National Laboratory (ORNL). These sensors provide completely passive, remote measurement capability. No power supplies, amplifiers, or other active components that could degrade system reliability are required at the sensor location. ORNL, through its research programs in scintillator materials, has developed a variety of materials for use in alpha-, beta-, gamma-, and neutron-sensitive scintillator detectors. In addition to sensors for measuring radiation flux, new sensor materials have been developed which are capable of measuring weight, temperature, and source location. An example of a passive sensor for temperature measurement is the combination of a thermophosphor (e.g., rare-earth activated Y 2 O 3 ) with 6 LiF (95% 6 Li). This combination results in a new class of scintillators for thermal neutrons that absorb energy from the radiation particles and remit the energy as a light pulse, the decay rate of which, over a specified temperature range, is temperature dependent. Other passive sensors being developed include pressure-sensitive triboluminescent materials, weight-sensitive silicone rubber fibers, scintillating fibers, and other materials for gamma and neutron detection. The light from the scintillator materials of each sensor would be sent through optical fibers to a monitoring station, where the attribute quantity could be measured and compared with previously recorded emission levels. Confirmatory measurement applications of these technologies are being evaluated to reduce the effort, costs, and employee exposures associated with inventorying stockpiles of highly enriched uranium at the Oak Ridge Y-12 Plant

  17. Wireless Integrated Microelectronic Vacuum Sensor System

    Science.gov (United States)

    Krug, Eric; Philpot, Brian; Trott, Aaron; Lawrence, Shaun

    2013-01-01

    NASA Stennis Space Center's (SSC's) large rocket engine test facility requires the use of liquid propellants, including the use of cryogenic fluids like liquid hydrogen as fuel, and liquid oxygen as an oxidizer (gases which have been liquefied at very low temperatures). These fluids require special handling, storage, and transfer technology. The biggest problem associated with transferring cryogenic liquids is product loss due to heat transfer. Vacuum jacketed piping is specifically designed to maintain high thermal efficiency so that cryogenic liquids can be transferred with minimal heat transfer. A vacuum jacketed pipe is essentially two pipes in one. There is an inner carrier pipe, in which the cryogenic liquid is actually transferred, and an outer jacket pipe that supports and seals the vacuum insulation, forming the "vacuum jacket." The integrity of the vacuum jacketed transmission lines that transfer the cryogenic fluid from delivery barges to the test stand must be maintained prior to and during engine testing. To monitor the vacuum in these vacuum jacketed transmission lines, vacuum gauge readings are used. At SSC, vacuum gauge measurements are done on a manual rotation basis with two technicians, each using a handheld instrument. Manual collection of vacuum data is labor intensive and uses valuable personnel time. Additionally, there are times when personnel cannot collect the data in a timely fashion (i.e., when a leak is detected, measurements must be taken more often). Additionally, distribution of this data to all interested parties can be cumbersome. To simplify the vacuum-gauge data collection process, automate the data collection, and decrease the labor costs associated with acquiring these measurements, an automated system that monitors the existing gauges was developed by Invocon, Inc. For this project, Invocon developed a Wireless Integrated Microelectronic Vacuum Sensor System (WIMVSS) that provides the ability to gather vacuum

  18. Optimal Sensor Selection for Health Monitoring Systems

    Science.gov (United States)

    Santi, L. Michael; Sowers, T. Shane; Aguilar, Robert B.

    2005-01-01

    Sensor data are the basis for performance and health assessment of most complex systems. Careful selection and implementation of sensors is critical to enable high fidelity system health assessment. A model-based procedure that systematically selects an optimal sensor suite for overall health assessment of a designated host system is described. This procedure, termed the Systematic Sensor Selection Strategy (S4), was developed at NASA John H. Glenn Research Center in order to enhance design phase planning and preparations for in-space propulsion health management systems (HMS). Information and capabilities required to utilize the S4 approach in support of design phase development of robust health diagnostics are outlined. A merit metric that quantifies diagnostic performance and overall risk reduction potential of individual sensor suites is introduced. The conceptual foundation for this merit metric is presented and the algorithmic organization of the S4 optimization process is described. Representative results from S4 analyses of a boost stage rocket engine previously under development as part of NASA's Next Generation Launch Technology (NGLT) program are presented.

  19. Enhanced technologies for unattended ground sensor systems

    Science.gov (United States)

    Hartup, David C.

    2010-04-01

    Progress in several technical areas is being leveraged to advantage in Unattended Ground Sensor (UGS) systems. This paper discusses advanced technologies that are appropriate for use in UGS systems. While some technologies provide evolutionary improvements, other technologies result in revolutionary performance advancements for UGS systems. Some specific technologies discussed include wireless cameras and viewers, commercial PDA-based system programmers and monitors, new materials and techniques for packaging improvements, low power cueing sensor radios, advanced long-haul terrestrial and SATCOM radios, and networked communications. Other technologies covered include advanced target detection algorithms, high pixel count cameras for license plate and facial recognition, small cameras that provide large stand-off distances, video transmissions of target activity instead of still images, sensor fusion algorithms, and control center hardware. The impact of each technology on the overall UGS system architecture is discussed, along with the advantages provided to UGS system users. Areas of analysis include required camera parameters as a function of stand-off distance for license plate and facial recognition applications, power consumption for wireless cameras and viewers, sensor fusion communication requirements, and requirements to practically implement video transmission through UGS systems. Examples of devices that have already been fielded using technology from several of these areas are given.

  20. Heimdall System for MSSS Sensor Tasking

    Science.gov (United States)

    Herz, A.; Jones, B.; Herz, E.; George, D.; Axelrad, P.; Gehly, S.

    In Norse Mythology, Heimdall uses his foreknowledge and keen eyesight to keep watch for disaster from his home near the Rainbow Bridge. Orbit Logic and the Colorado Center for Astrodynamics Research (CCAR) at the University of Colorado (CU) have developed the Heimdall System to schedule observations of known and uncharacterized objects and search for new objects from the Maui Space Surveillance Site. Heimdall addresses the current need for automated and optimized SSA sensor tasking driven by factors associated with improved space object catalog maintenance. Orbit Logic and CU developed an initial baseline prototype SSA sensor tasking capability for select sensors at the Maui Space Surveillance Site (MSSS) using STK and STK Scheduler, and then added a new Track Prioritization Component for FiSST-inspired computations for predicted Information Gain and Probability of Detection, and a new SSA-specific Figure-of-Merit (FOM) for optimized SSA sensor tasking. While the baseline prototype addresses automation and some of the multi-sensor tasking optimization, the SSA-improved prototype addresses all of the key elements required for improved tasking leading to enhanced object catalog maintenance. The Heimdall proof-of-concept was demonstrated for MSSS SSA sensor tasking for a 24 hour period to attempt observations of all operational satellites in the unclassified NORAD catalog, observe a small set of high priority GEO targets every 30 minutes, make a sky survey of the GEO belt region accessible to MSSS sensors, and observe particular GEO regions that have a high probability of finding new objects with any excess sensor time. This Heimdall prototype software paves the way for further R&D that will integrate this technology into the MSSS systems for operational scheduling, improve the software's scalability, and further tune and enhance schedule optimization. The Heimdall software for SSA sensor tasking provides greatly improved performance over manual tasking, improved

  1. Integrated Microfluidic Sensor System with Magnetostrictive Resonators

    KAUST Repository

    Liang, Cai; Kosel, Jü rgen; Gooneratne, Chinthaka

    2011-01-01

    The present embodiments describe a method that integrates a magnetostrictive sensor with driving and detecting elements into a microfluidic chip to detect a chemical, biochemical or biomedical species. These embodiments may also measure the properties of a fluid such as viscosity, pH values. The whole system can be referred to lab-on-a-chip (LOC) or micro-total-analysis-systems (.mu.TAS). In particular, this present embodiments include three units, including a microfluidics unit, a magnetostrictive sensor, and driving/detecting elements. An analyzer may also be provided to analyze an electrical signal associated with a feature of a target specimen.

  2. Multimodal surveillance sensors, algorithms, and systems

    CERN Document Server

    Zhu, Zhigang

    2007-01-01

    From front-end sensors to systems and environmental issues, this practical resource guides you through the many facets of multimodal surveillance. The book examines thermal, vibration, video, and audio sensors in a broad context of civilian and military applications. This cutting-edge volume provides an in-depth treatment of data fusion algorithms that takes you to the core of multimodal surveillance, biometrics, and sentient computing. The book discusses such people and activity topics as tracking people and vehicles and identifying individuals by their speech.Systems designers benefit from d

  3. Integrated Microfluidic Sensor System with Magnetostrictive Resonators

    KAUST Repository

    Liang, Cai

    2011-12-08

    The present embodiments describe a method that integrates a magnetostrictive sensor with driving and detecting elements into a microfluidic chip to detect a chemical, biochemical or biomedical species. These embodiments may also measure the properties of a fluid such as viscosity, pH values. The whole system can be referred to lab-on-a-chip (LOC) or micro-total-analysis-systems (.mu.TAS). In particular, this present embodiments include three units, including a microfluidics unit, a magnetostrictive sensor, and driving/detecting elements. An analyzer may also be provided to analyze an electrical signal associated with a feature of a target specimen.

  4. Exploring a minimal two-component p53 model

    International Nuclear Information System (INIS)

    Sun, Tingzhe; Zhu, Feng; Shen, Pingping; Yuan, Ruoshi; Xu, Wei

    2010-01-01

    The tumor suppressor p53 coordinates many attributes of cellular processes via interlocked feedback loops. To understand the biological implications of feedback loops in a p53 system, a two-component model which encompasses essential feedback loops was constructed and further explored. Diverse bifurcation properties, such as bistability and oscillation, emerge by manipulating the feedback strength. The p53-mediated MDM2 induction dictates the bifurcation patterns. We first identified irradiation dichotomy in p53 models and further proposed that bistability and oscillation can behave in a coordinated manner. Further sensitivity analysis revealed that p53 basal production and MDM2-mediated p53 degradation, which are central to cellular control, are most sensitive processes. Also, we identified that the much more significant variations in amplitude of p53 pulses observed in experiments can be derived from overall amplitude parameter sensitivity. The combined approach with bifurcation analysis, stochastic simulation and sampling-based sensitivity analysis not only gives crucial insights into the dynamics of the p53 system, but also creates a fertile ground for understanding the regulatory patterns of other biological networks

  5. Sensors

    CERN Document Server

    Pigorsch, Enrico

    1997-01-01

    This is the 5th edition of the Metra Martech Directory "EUROPEAN CENTRES OF EXPERTISE - SENSORS." The entries represent a survey of European sensors development. The new edition contains 425 detailed profiles of companies and research institutions in 22 countries. This is reflected in the diversity of sensors development programmes described, from sensors for physical parameters to biosensors and intelligent sensor systems. We do not claim that all European organisations developing sensors are included, but this is a good cross section from an invited list of participants. If you see gaps or omissions, or would like your organisation to be included, please send details. The data base invites the formation of effective joint ventures by identifying and providing access to specific areas in which organisations offer collaboration. This issue is recognised to be of great importance and most entrants include details of collaboration offered and sought. We hope the directory on Sensors will help you to find the ri...

  6. Bioinspired optical sensors for unmanned aerial systems

    Science.gov (United States)

    Chahl, Javaan; Rosser, Kent; Mizutani, Akiko

    2011-04-01

    Insects are dependant on the spatial, spectral and temporal distributions of light in the environment for flight control and navigation. This paper reports on flight trials of implementations of insect inspired behaviors on unmanned aerial vehicles. Optical flow methods for maintaining a constant height above ground and a constant course have been demonstrated to provide navigation capabilities that are impossible using conventional avionics sensors. Precision control of height above ground and ground course were achieved over long distances. Other vision based techniques demonstrated include a biomimetic stabilization sensor that uses the ultraviolet and green bands of the spectrum, and a sky polarization compass. Both of these sensors were tested over long trajectories in different directions, in each case showing performance similar to low cost inertial heading and attitude systems. The behaviors demonstrate some of the core functionality found in the lower levels of the sensorimotor system of flying insects and shows promise for more integrated solutions in the future.

  7. Data acquisition for sensor systems

    CERN Document Server

    Taylor, H Rosemary

    1997-01-01

    'Data acquisition' is concerned with taking one or more analogue signals and converting them to digital form with sufficient accu­ racy and speed to be ready for processing by a computer. The increasing use of computers makes this an expanding field, and it is important that the conversion process is done correctly because information lost at this stage can never be regained, no matter how good the computation. The old saying - garbage in, garbage out - is very relevant to data acquisition, and so every part of the book contains a discussion of errors: where do they come from, how large are they, and what can be done to reduce them? The book aims to treat the data acquisition process in depth with less detailed chapters on the fundamental principles of measure­ ment, sensors and signal conditioning. There is also a chapter on software packages, which are becoming increasingly popular. This is such a rapidly changing topic that any review of available pro­ grams is bound to be out of date before the book re...

  8. Wearable PPG sensor based alertness scoring system.

    Science.gov (United States)

    Dey, Jishnu; Bhowmik, Tanmoy; Sahoo, Saswata; Tiwari, Vijay Narayan

    2017-07-01

    Quantifying mental alertness in today's world is important as it enables the person to adopt lifestyle changes for better work efficiency. Miniaturized sensors in wearable devices have facilitated detection/monitoring of mental alertness. Photoplethysmography (PPG) sensors through Heart Rate Variability (HRV) offer one such opportunity by providing information about one's daily alertness levels without requiring any manual interference from the user. In this paper, a smartwatch based alertness estimation system is proposed. Data collected from PPG sensor of smartwatch is processed and fed to machine learning based model to get a continuous alertness score. Utility functions are designed based on statistical analysis to give a quality score on different stages of alertness such as awake, long sleep and short duration power nap. An intelligent data collection approach is proposed in collaboration with the motion sensor in the smartwatch to reduce battery drainage. Overall, our proposed wearable based system provides a detailed analysis of alertness over a period in a systematic and optimized manner. We were able to achieve an accuracy of 80.1% for sleep/awake classification along with alertness score. This opens up the possibility for quantifying alertness levels using a single PPG sensor for better management of health related activities including sleep.

  9. Narrow field electromagnetic sensor system and method

    International Nuclear Information System (INIS)

    McEwan, T.E.

    1996-01-01

    A narrow field electromagnetic sensor system and method of sensing a characteristic of an object provide the capability to realize a characteristic of an object such as density, thickness, or presence, for any desired coordinate position on the object. One application is imaging. The sensor can also be used as an obstruction detector or an electronic trip wire with a narrow field without the disadvantages of impaired performance when exposed to dirt, snow, rain, or sunlight. The sensor employs a transmitter for transmitting a sequence of electromagnetic signals in response to a transmit timing signal, a receiver for sampling only the initial direct RF path of the electromagnetic signal while excluding all other electromagnetic signals in response to a receive timing signal, and a signal processor for processing the sampled direct RF path electromagnetic signal and providing an indication of the characteristic of an object. Usually, the electromagnetic signal is a short RF burst and the obstruction must provide a substantially complete eclipse of the direct RF path. By employing time-of-flight techniques, a timing circuit controls the receiver to sample only the initial direct RF path of the electromagnetic signal while not sampling indirect path electromagnetic signals. The sensor system also incorporates circuitry for ultra-wideband spread spectrum operation that reduces interference to and from other RF services while allowing co-location of multiple electronic sensors without the need for frequency assignments. 12 figs

  10. Composite fermion basis for two-component Bose gases

    Science.gov (United States)

    Meyer, Marius; Liabotro, Ola

    The composite fermion (CF) construction is known to produce wave functions that are not necessarily orthogonal, or even linearly independent, after projection. While usually not a practical issue in the quantum Hall regime, we have previously shown that it presents a technical challenge for rotating Bose gases with low angular momentum. These are systems where the CF approach yield surprisingly good approximations to the exact eigenstates of weak short-range interactions, and so solving the problem of linearly dependent wave functions is of interest. It can also be useful for studying CF excitations for fermions. Here we present several ways of constructing a basis for the space of ``simple CF states'' for two-component rotating Bose gases in the lowest Landau level, and prove that they all give a basis. Using the basis, we study the structure of the lowest-lying state using so-called restricted wave functions. We also examine the scaling of the overlap between the exact and CF wave functions at the maximal possible angular momentum for simple states. This work was financially supported by the Research Council of Norway.

  11. Flood early warning system: sensors and internet

    NARCIS (Netherlands)

    Pengel, B.E.; Krzhizhanovskaya, V.V.; Melnikova, N.B.; Shirshov, G.S.; Koelewijn, A.R.; Pyayt, A.L.; Mokhov, I.I.; Chavoshian, A.; Takeuchi, K.

    2013-01-01

    The UrbanFlood early warning system (EWS) is designed to monitor data from very large sensornetworks in flood defences such as embankments, dikes, levees, and dams. The EWS, based on the internet, uses real-time sensor information and Artificial Intelligence (AI) to immediately calculate the

  12. Stressor sensor and stress management system

    NARCIS (Netherlands)

    2012-01-01

    A stressor detection system (100) comprises sensor means (101) arranged for being attached to a person for obtaining a time-varying signal representing a physical quantity relating to an environment of the person, and processing means (102) for deriving a stressor value from the obtained signal

  13. Optimization of wireless Bluetooth sensor systems.

    Science.gov (United States)

    Lonnblad, J; Castano, J; Ekstrom, M; Linden, M; Backlund, Y

    2004-01-01

    Within this study, three different Bluetooth sensor systems, replacing cables for transmission of biomedical sensor data, have been designed and evaluated. The three sensor architectures are built on 1-, 2- and 3-chip solutions and depending on the monitoring situation and signal character, different solutions are optimal. Essential parameters for all systems have been low physical weight and small size, resistance to interference and interoperability with other technologies as global- or local networks, PC's and mobile phones. Two different biomedical input signals, ECG and PPG (photoplethysmography), have been used to evaluate the three solutions. The study shows that it is possibly to continuously transmit an analogue signal. At low sampling rates and slowly varying parameters, as monitoring the heart rate with PPG, the 1-chip solution is the most suitable, offering low power consumption and thus a longer battery lifetime or a smaller battery, minimizing the weight of the sensor system. On the other hand, when a higher sampling rate is required, as an ECG, the 3-chip architecture, with a FPGA or micro-controller, offers the best solution and performance. Our conclusion is that Bluetooth might be useful in replacing cables of medical monitoring systems.

  14. Complexation in two-component chlortetracycline-melanin solutions

    Science.gov (United States)

    Lapina, V. A.; Pershukevich, P. P.; Dontsov, A. E.; Bel'Kov, M. V.

    2008-01-01

    The spectra and kinetics of fluorescence of two-component solutions of the chlortetracycline (CHTC)-DOPA-melanin (melanin or ME) system in water have been investigated. The data obtained have been compared to similar data for solutions of CHTC-melanosome from bull eye (MB), which contains natural melanin, in K-phosphate buffer at pH 7.4. The overall results indicate the occurrence of complexation between molecules of CHTC and ME as they are being excited. The studies of complexation in the solution of CHTC-MB in the buffer are complicated by the formation of a CHTC-buffer complex. The effect of optical radiation in the range 330-750 nm on the CHTC-ME complex shows selectivity: the greatest change in the spectrum occurs when the wavelength of the exciting radiation coincides with the long-wavelength band maximum of the fluorescence excitation spectrum of the CHTC-ME complex in aqueous solution. In this range, CHTC and especially ME show high photochemical stability. The nature of the radiation effect on the studied compounds in the hard UV range (λ < 330 nm) differs greatly from that in the range 330-750 nm. It is apparently accompanied by significant photochemical transmutations of all system components. By comparing the characteristics of the CHTC-ME systems with those of the related drug doxycycline (DC-ME), the conclusion has been made that the chlorine atom plays a vital role in formation of the short-wavelength band in the fluorescence spectrum of the CHTC-ME complex.

  15. Wireless Sensor Network Based Smart Parking System

    Directory of Open Access Journals (Sweden)

    Jeffrey JOSEPH

    2014-01-01

    Full Text Available Ambient Intelligence is a vision in which various devices come together and process information from multiple sources in order to exert control on the physical environment. In addition to computation and control, communication plays a crucial role in the overall functionality of such a system. Wireless Sensor Networks are one such class of networks, which meet these criteria. These networks consist of spatially distributed sensor motes which work in a co-operative manner to sense and control the environment. In this work, an implementation of an energy-efficient and cost-effective, wireless sensor networks based vehicle parking system for a multi-floor indoor parking facility has been introduced. The system monitors the availability of free parking slots and guides the vehicle to the nearest free slot. The amount of time the vehicle has been parked is monitored for billing purposes. The status of the motes (dead/alive is also recorded. Information like slot allocated, directions to the slot and billing data is sent as a message to customer’s mobile phones. This paper extends our previous work 1 with the development of a low cost sensor mote, about one tenth the cost of a commercially available mote, keeping in mind the price sensitive markets of the developing countries.

  16. Adaptive Sensing Based on Profiles for Sensor Systems

    Directory of Open Access Journals (Sweden)

    Yoshiteru Ishida

    2009-10-01

    Full Text Available This paper proposes a profile-based sensing framework for adaptive sensor systems based on models that relate possibly heterogeneous sensor data and profiles generated by the models to detect events. With these concepts, three phases for building the sensor systems are extracted from two examples: a combustion control sensor system for an automobile engine, and a sensor system for home security. The three phases are: modeling, profiling, and managing trade-offs. Designing and building a sensor system involves mapping the signals to a model to achieve a given mission.

  17. Advanced sensor systems for biotelemetry

    Science.gov (United States)

    Hines, John W. (Inventor); Somps, Christopher J. (Inventor); Ricks, Robert D. (Inventor); Mundt, Carsten W. (Inventor)

    2003-01-01

    The present invention relates to telemetry-based sensing systems that continuously measures physical, chemical and biological parameters. More specifically, these sensing systems comprise a small, modular, low-power implantable biotelemetry system capable of continuously sensing physiological characteristics using implantable transmitters, a receiver, and a data acquisition system to analyze and record the transmitted signal over several months. The preferred embodiment is a preterm labor and fetal monitoring system. Key features of the invention include Pulse Interval Modulation (PIM) that is used to send temperature and pressure information out of the biological environment. The RF carrier frequency is 174-216 MHz and a pair of RF bursts (pulses) is transmitted at a frequency of about 1-2 Hz. The transmission range is 3 to 10 feet, depending on the position of the transmitter in the body and its biological environment. The entire transmitter is encapsulated in biocompatible silicone rubber. Power is supplied by on-board silver-oxide batteries. The average power consumption of the current design is less than 30 .mu.W., which yields a lifetime of approximately 6-9 months. Chip-on-Board technology (COB) drastically reduces the size of the printed circuit board from 38.times.28 mm to 22.times.8 mm. Unpackaged dies are flip-chip bonded directly onto the printed circuit board, along with surface mount resistors and capacitors. The invention can monitor additional physiological parameters including, but not limited to, ECG, blood gases, glucose, and ions such as calcium, potassium, and sodium.

  18. Itinerant Ferromagnetism in a Polarized Two-Component Fermi Gas

    DEFF Research Database (Denmark)

    Massignan, Pietro; Yu, Zhenhua; Bruun, Georg

    2013-01-01

    We analyze when a repulsively interacting two-component Fermi gas becomes thermodynamically unstable against phase separation. We focus on the strongly polarized limit, where the free energy of the homogeneous mixture can be calculated accurately in terms of well-defined quasiparticles, the repul......We analyze when a repulsively interacting two-component Fermi gas becomes thermodynamically unstable against phase separation. We focus on the strongly polarized limit, where the free energy of the homogeneous mixture can be calculated accurately in terms of well-defined quasiparticles...

  19. On-line methanol sensor system development for recombinant ...

    African Journals Online (AJOL)

    On-line methanol sensor system development for recombinant human serum ... of the methanol sensor system was done in a medium environment with yeast cells ... induction at a low temperature and a pH where protease does not function.

  20. Bio-integrated electronics and sensor systems

    Science.gov (United States)

    Yeo, Woon-Hong; Webb, R. Chad; Lee, Woosik; Jung, Sungyoung; Rogers, John A.

    2013-05-01

    Skin-mounted epidermal electronics, a strategy for bio-integrated electronics, provide an avenue to non-invasive monitoring of clinically relevant physiological signals for healthcare applications. Current conventional systems consist of single-point sensors fastened to the skin with adhesives, and sometimes with conducting gels, which limits their use outside of clinical settings due to loss of adhesion and irritation to the user. In order to facilitate extended use of skin-mounted healthcare sensors without disrupting everyday life, we envision electronic monitoring systems that integrate seamlessly with the skin below the notice of the user. This manuscript reviews recent significant results towards our goal of wearable electronic sensor systems for long-term monitoring of physiological signals. Ultra-thin epidermal electronic systems (EES) are demonstrated for extended use on the skin, in a conformal manner, including during everyday bathing and sleeping activities. We describe the assessment of clinically relevant physiological parameters, such as electrocardiograms (ECG), electromyograms (EMG), electroencephalograms (EEG), temperature, mechanical strain and thermal conductivity, using examples of multifunctional EES devices. Additionally, we demonstrate capability for real life application of EES by monitoring the system functionality, which has no discernible change, during cyclic fatigue testing.

  1. Sensor Systems Collect Critical Aerodynamics Data

    Science.gov (United States)

    2010-01-01

    With the support of Small Business Innovation Research (SBIR) contracts with Dryden Flight Research Center, Tao of Systems Integration Inc. developed sensors and other components that will ultimately form a first-of-its-kind, closed-loop system for detecting, measuring, and controlling aerodynamic forces and moments in flight. The Hampton, Virginia-based company commercialized three of the four planned components, which provide sensing solutions for customers such as Boeing, General Electric, and BMW and are used for applications such as improving wind turbine operation and optimizing air flow from air conditioning systems. The completed system may one day enable flexible-wing aircraft with flight capabilities like those of birds.

  2. Bacitracin Resistance of Streptococcus mutans Regulated by Two Component System

    OpenAIRE

    北河, 憲雄

    2011-01-01

    Streptococcus mutansは、主要なう蝕の原因細菌であり、バシトラシンに耐性を持つことが知られている。本研究では、この耐性メカニズムを解明するため、バシトラシン存在下で顕著に発現誘導される遺伝子をマイクロアレイを用いて探索した。4 倍以上発現誘導されていた8 つの遺伝子のうち、耐性に主に関与していたのは ABC トランスポーターをコードすると推定される 2 つの遺伝子、mbrA、mbrB であった。以前の研究から、mbrABCD 遺伝子群はバシトラシン耐性に関与することが分かっているが mbrABCD 遺伝子群による耐性のメカニズムの詳細は判明していない。アミノ酸配列のホモロジーより mbrCD は 二成分制御系 (TCS) の遺伝子と推測されており、mbrC、mbrD それぞれの欠損株を作製して検討したところ、推測通り、 mbrCD が mbrA の発現を制御していた。また、ゲルシフトアッセイにより MbrC はmbrA の発現調節領域と推定される部位と特異的に結合すること、及びリン酸化部位と予測される 54 番目のアスパラギン酸をアスパラギンに置換した変異 Mb...

  3. Analytical static structure factor for a two-component system ...

    Indian Academy of Sciences (India)

    Marwan Al-Raeei

    2018-03-29

    Mar 29, 2018 ... be useful in studying biomolecular fluids and other soft matter fluids. Keywords. Ornstein–Zernike ... partial structure factor; isothermal compressibility; soft matter. PACS No. 05.20.Jj. 1. ..... computing. Users need to have ...

  4. Handbook of sensor networks compact wireless and wired sensing systems

    CERN Document Server

    Ilyas, Mohammad

    2004-01-01

    INTRODUCTION Opportunities and Challenges in Wireless Sensor Networks, M. Haenggi, Next Generation Technologies to Enable Sensor Networks, J. I.  Goodman, A. I. Reuther, and D. R. Martinez Sensor Networks Management, L. B. Ruiz, J. M. Nogueira, and A. A. F. Loureiro Models for Programmability in Sensor Networks, A. Boulis Miniaturizing Sensor Networks with MEMS, Brett Warneke A Taxonomy of Routing Techniques in Wireless Sensor Networks, J. N. Al-Karaki and A. E. Kamal Artificial Perceptual Systems, A. Loutfi, M. Lindquist, and P. Wide APPLICATIONS Sensor Network Architecture and Appl

  5. Sensor-based material tagging system

    International Nuclear Information System (INIS)

    Vercellotti, L.C.; Cox, R.W.; Ravas, R.J.; Schlotterer, J.C.

    1991-01-01

    Electronic identification tags are being developed for tracking material and personnel. In applying electronic identification tags to radioactive materials safeguards, it is important to measure attributes of the material to ensure that the tag remains with the material. The addition of a microcontroller with an on-board analog-to-digital converter to an electronic identification tag application-specific integrated-circuit has been demonstrated as means to provide the tag with sensor data. Each tag is assembled into a housing, which serves as a scale for measuring the weight of a paint-can-sized container and its contents. Temperature rise of the can above ambient is also measured, and a piezoelectric detector detects disturbances and immediately puts the tag into its alarm and beacon mode. Radiation measurement was also considered, but the background from nearby containers was found to be excessive. The sensor-based tagging system allows tracking of the material in cans as it is stored in vaults or is moved through the manufacturing process. The paper presents details of the sensor-based material tagging system and describes a demonstration system

  6. Alcohol control: Mobile sensor system and numerical signal analysis

    OpenAIRE

    Seifert, Rolf; Keller, Hubert B.; Conrad, Thorsten; Peter, Jens

    2016-01-01

    An innovative mobile sensor system for alcohol control in the respiratory air is introduced. The gas sensor included in the sensor system is thermo-cyclically operated. Ethanol is the leading component in this context. However, other components occur in the breathing air which can influence the concentration determination of ethanol. Therefore, mono- ethanol samples and binary gas mixtures are measured by the sensor system and analyzed with a new calibration and evaluation procedure which is ...

  7. System-level Modeling of Wireless Integrated Sensor Networks

    DEFF Research Database (Denmark)

    Virk, Kashif M.; Hansen, Knud; Madsen, Jan

    2005-01-01

    Wireless integrated sensor networks have emerged as a promising infrastructure for a new generation of monitoring and tracking applications. In order to efficiently utilize the extremely limited resources of wireless sensor nodes, accurate modeling of the key aspects of wireless sensor networks...... is necessary so that system-level design decisions can be made about the hardware and the software (applications and real-time operating system) architecture of sensor nodes. In this paper, we present a SystemC-based abstract modeling framework that enables system-level modeling of sensor network behavior...... by modeling the applications, real-time operating system, sensors, processor, and radio transceiver at the sensor node level and environmental phenomena, including radio signal propagation, at the sensor network level. We demonstrate the potential of our modeling framework by simulating and analyzing a small...

  8. A hybrid two-component Bose–Einstein condensate interferometer for measuring magnetic field gradients

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Fei [Key Laboratory of Fiber Optic Sensing Technology and Information Processing, Ministry of Education, Wuhan University of Technology, Wuhan 430070 (China); Huang, Jiahao, E-mail: hjiahao@mail2.sysu.edu.cn [TianQin Research Center & School of Physics and Astronomy, Sun Yat-Sen University, SYSU Zhuhai Campus, Zhuhai 519082 (China); Liu, Quan [Key Laboratory of Fiber Optic Sensing Technology and Information Processing, Ministry of Education, Wuhan University of Technology, Wuhan 430070 (China)

    2017-03-03

    Highlights: • A scheme for detecting magnetic field gradients via a double-well two-component Bose–Einstein condensate interferometer. • The magnetic field gradient can be extracted by either the spin population or the external state. • Our proposal is potentially sensitive to weak magnetic field inhomogeneity due to its small sensor size. - Abstract: We have proposed a scheme to detect magnetic field gradients via an interferometer based on a double-well two-component Bose–Einstein condensate (BEC). Utilizing a sequence of quantum control operations on both external and internal degree of the BEC, one can extract the magnetic field gradients by measuring either the population in one component or the fidelity between the final external state and the initial ground state. Our scheme can be implemented by current experimental techniques of manipulating ultracold atoms.

  9. Alcohol Control: Mobile Sensor System and Numerical Signal Analysis

    Directory of Open Access Journals (Sweden)

    Rolf SEIFERT

    2016-10-01

    Full Text Available An innovative mobile sensor system for alcohol control in the respiratory air is introduced. The gas sensor included in the sensor system is thermo-cyclically operated. Ethanol is the leading component in this context. However, other components occur in the breathing air which can influence the concentration determination of ethanol. Therefore, mono- ethanol samples and binary gas mixtures are measured by the sensor system and analyzed with a new calibration and evaluation procedure which is also incorporated in the system. The applications demonstrate a good substance identification capability of the sensor system and a very good concentration determination of the components.

  10. One-port portable SAW sensor system

    Science.gov (United States)

    Hoa Nguyen, Vu; Peters, Oliver; Schnakenberg, Uwe

    2018-01-01

    A portable device using the SAW-based impedance sensor type based on one interdigital transducer simultaneously as SAW generator and sensor element (1-port approach) is introduced. As a novelty, the so far required expensive vector network analyzer (VNA) is replaced by a hand-held device to measure the impedance spectrum of the SAW sensor by RF-gain-phase meters. Hence, some of the best features from the conventional oscillator and VNA approaches are combined to develop a low-cost and self-contained measurement system, including signal in- and output ability for real-time measurements. The pivotal aspect of the portable system is the transfer of the sophisticated high frequency approach into a quasi-static one. This enables the use of simple lumped electronics without the need of impedance matching circuits. Proof-of-concept was carried out by measuring conductivities of phosphate-buffered solutions and viscosities of glycerin. Sensitivities for temperature of 0.3%/°C, viscosity of 10.1% (mPa s)-1 and conductivity of 0.5% (S cm)-1 have been determined, respectively, which are competitive results compared to the benchmark approaches.

  11. A two-component NZRI metamaterial based rectangular cloak

    Directory of Open Access Journals (Sweden)

    Sikder Sunbeam Islam

    2015-10-01

    Full Text Available A new two-component, near zero refractive index (NZRI metamaterial is presented for electromagnetic rectangular cloaking operation in the microwave range. In the basic design a pi-shaped, metamaterial was developed and its characteristics were investigated for the two major axes (x and z-axis wave propagation through the material. For the z-axis wave propagation, it shows more than 2 GHz bandwidth and for the x-axis wave propagation; it exhibits more than 1 GHz bandwidth of NZRI property. The metamaterial was then utilized in designing a rectangular cloak where a metal cylinder was cloaked perfectly in the C-band area of microwave regime. The experimental result was provided for the metamaterial and the cloak and these results were compared with the simulated results. This is a novel and promising design for its two-component NZRI characteristics and rectangular cloaking operation in the electromagnetic paradigm.

  12. On the Alexander polynominals of alternating two-component links

    Directory of Open Access Journals (Sweden)

    Mark E. Kidwell

    1979-01-01

    Full Text Available Let L be an alternating two-component link with Alexander polynomial Δ(x,y. Then the polynomials (1−xΔ(x,y and (1−yΔ(x,y are alternating. That is, (1−yΔ(x,y can be written as ∑i,jcijxiyj in such a way that (−1i+jcij≥0.

  13. Underwater Animal Monitoring Magnetic Sensor System

    KAUST Repository

    Kaidarova, Altynay

    2017-10-01

    Obtaining new insights into the behavior of free-living marine organisms is fundamental for conservation efforts and anticipating the impact of climate change on marine ecosystems. Despite the recent advances in biotelemetry, collecting physiological and behavioral parameters of underwater free-living animals remains technically challenging. In this thesis, we develop the first magnetic underwater animal monitoring system that utilizes Tunnel magnetoresistance (TMR) sensors, the most sensitive solid-state sensors today, coupled with flexible magnetic composites. The TMR sensors are composed of CoFeB free layers and MgO tunnel barriers, patterned using standard optical lithography and ion milling procedures. The short and long-term stability of the TMR sensors has been studied using statistical and Allan deviation analysis. Instrumentation noise has been reduced using optimized electrical interconnection schemes. We also develop flexible NdFeB-PDMS composite magnets optimized for applications in corrosive marine environments, and which can be attached to marine animals. The magnetic and mechanical properties are studied for different NdFeB powder concentrations and the performance of the magnetic composites for different exposure times to sea water is systematically investigated. Without protective layer, the composite magnets loose more than 50% of their magnetization after 51 days in seawater. The durability of the composite magnets can be considerably improved by using polymer coatings which are protecting the composite magnet, whereby Parylene C is found to be the most effective solution, providing simultaneously corrosion resistance, flexibility, and enhanced biocompatibility. A Parylene C film of 2μm thickness provides the sufficient protection of the magnetic composite in corrosive aqueous environments for more than 70 days. For the high level performance of the system, the theoretically optimal position of the composite magnets with respect to the sensing

  14. BABY MONITORING SYSTEM USING WIRELESS SENSOR NETWORKS

    Directory of Open Access Journals (Sweden)

    G. Rajesh

    2014-09-01

    Full Text Available Sudden Infant Death Syndrome (SIDS is marked by the sudden death of an infant during sleep that is not predicted by the medical history and remains unexplained even after thorough forensic autopsy and detailed death investigation. In this we developed a system that provides solutions for the above problems by making the crib smart using the wireless sensor networks (WSN and smart phones. The system provides visual monitoring service through live video, alert services by crib fencing and awakens alert, monitoring services by temperature reading and light intensity reading, vaccine reminder and weight monitoring.

  15. Two-component gravitational instability in spiral galaxies

    Science.gov (United States)

    Marchuk, A. A.; Sotnikova, N. Y.

    2018-04-01

    We applied a criterion of gravitational instability, valid for two-component and infinitesimally thin discs, to observational data along the major axis for seven spiral galaxies of early types. Unlike most papers, the dispersion equation corresponding to the criterion was solved directly without using any approximation. The velocity dispersion of stars in the radial direction σR was limited by the range of possible values instead of a fixed value. For all galaxies, the outer regions of the disc were analysed up to R ≤ 130 arcsec. The maximal and sub-maximal disc models were used to translate surface brightness into surface density. The largest destabilizing disturbance stars can exert on a gaseous disc was estimated. It was shown that the two-component criterion differs a little from the one-fluid criterion for galaxies with a large surface gas density, but it allows to explain large-scale star formation in those regions where the gaseous disc is stable. In the galaxy NGC 1167 star formation is entirely driven by the self-gravity of the stars. A comparison is made with the conventional approximations which also include the thickness effect and with models for different sound speed cg. It is shown that values of the effective Toomre parameter correspond to the instability criterion of a two-component disc Qeff < 1.5-2.5. This result is consistent with previous theoretical and observational studies.

  16. Online Sensor Calibration Assessment in Nuclear Power Systems

    International Nuclear Information System (INIS)

    Coble, Jamie B.; Ramuhalli, Pradeep; Meyer, Ryan M.; Hashemian, Hash

    2013-01-01

    Safe, efficient, and economic operation of nuclear systems (nuclear power plants, fuel fabrication and storage, used fuel processing, etc.) relies on transmission of accurate and reliable measurements. During operation, sensors degrade due to age, environmental exposure, and maintenance interventions. Sensor degradation can affect the measured and transmitted signals, including sensor failure, signal drift, sensor response time, etc. Currently, periodic sensor recalibration is performed to avoid these problems. Sensor recalibration activities include both calibration assessment and adjustment (if necessary). In nuclear power plants, periodic recalibration of safety-related sensors is required by the plant technical specifications. Recalibration typically occurs during refueling outages (about every 18 to 24 months). Non-safety-related sensors also undergo recalibration, though not as frequently. However, this approach to maintaining sensor calibration and performance is time-consuming and expensive, leading to unnecessary maintenance, increased radiation exposure to maintenance personnel, and potential damage to sensors. Online monitoring (OLM) of sensor performance is a non-invasive approach to assess instrument calibration. OLM can mitigate many of the limitations of the current periodic recalibration practice by providing more frequent assessment of calibration and identifying those sensors that are operating outside of calibration tolerance limits without removing sensors or interrupting operation. This can support extended operating intervals for unfaulted sensors and target recalibration efforts to only degraded sensors

  17. A protein-dye hybrid system as a narrow range tunable intracellular pH sensor.

    Science.gov (United States)

    Anees, Palapuravan; Sudheesh, Karivachery V; Jayamurthy, Purushothaman; Chandrika, Arunkumar R; Omkumar, Ramakrishnapillai V; Ajayaghosh, Ayyappanpillai

    2016-11-18

    Accurate monitoring of pH variations inside cells is important for the early diagnosis of diseases such as cancer. Even though a variety of different pH sensors are available, construction of a custom-made sensor array for measuring minute variations in a narrow biological pH window, using easily available constituents, is a challenge. Here we report two-component hybrid sensors derived from a protein and organic dye nanoparticles whose sensitivity range can be tuned by choosing different ratios of the components, to monitor the minute pH variations in a given system. The dye interacts noncovalently with the protein at lower pH and covalently at higher pH, triggering two distinguishable fluorescent signals at 700 and 480 nm, respectively. The pH sensitivity region of the probe can be tuned for every unit of the pH window resulting in custom-made pH sensors. These narrow range tunable pH sensors have been used to monitor pH variations in HeLa cells using the fluorescence imaging technique.

  18. Synthesis and Characterization of Two Component Alloy Nanoparticles

    Science.gov (United States)

    Tabatabaei, Salomeh

    Alloying is an old trick used to produce new materials by synergistically combining at least two components. New developments in nanoscience have enabled new degrees of freedom, such as size, solubility and concentration of the alloying element to be utilized in the design of the physical properties of alloy nanoparticles (ANPs). ANPs as multi-functional materials have applications in catalysis, biomedical technologies and electronics. Phase diagrams of ANPs are very little known and may not represent that of bulk picture, furthermore, ANPs with different crystallite orientation and compositions could remain far from equilibrium. Here, we studied the synthesis and stability of Au-Sn and Ag-Ni ANPs with chemical reduction method at room temperature. Due to the large difference in the redox potentials of Au and Sn, co-reduction is not a reproducible method. However, two step successive reductions was found to be more reliable to generate Au-Sn ANPs which consists of forming clusters in the first step (either without capping agent or with weakly coordinated surfactant molecules) and then undergoing a second reduction step in the presence of another metal salt. Our observation also showed that capping agents (Cetrimonium bromide or (CTAB)) and Polyacrylic acid (PAA)) play a key role in the alloying process and shorter length capping agent (PAA) may facilitate the diffusion of individual components and thus enabling better alloying. Different molar ratios of Sn and Au precursors were used to study the effect of alloying elements on the melting point and the crystalline structures and melting points were determined by various microscopy and spectroscopy techniques and differential scanning calorimetry (DSC). A significant depression (up to150°C) in the melting transition was observed for the Au-Sn ANPs compared to the bulk eutectic point (Tm 280°C) due to the size and shape effect. Au-Sn ANPs offer a unique set of advantages as lead-free solder material which can

  19. Smart sensor systems for outdoor intrusion detection

    International Nuclear Information System (INIS)

    Lynn, J.K.

    1988-01-01

    A major improvement in outdoor perimeter security system probability of detection (PD) and reduction in false alarm rate (FAR) and nuisance alarm rate (NAR) may be obtained by analyzing the indications immediately preceding an event which might be interpreted as an intrusion. Existing systems go into alarm after crossing a threshold. Very slow changes, which accumulate until the threshold is reached, may be assessed falsely as an intrusion. A hierarchial program has begun at Stellar to develop a modular, expandable Smart Sensor system which may be interfaced to most types of sensor and alarm reporting systems. A major upgrade to the SSI Test Site is in progress so that intrusions may be simulated in a controlled and repeatable manner. A test platform is being constructed which will operate in conduction with a mobile instrumentation center with CCTVB, lighting control, weather and data monitoring and remote control of the test platform and intrusion simulators. Additional testing was contracted with an independent test facility to assess the effects of severe winter weather conditions

  20. Development of wireless sensor network for landslide monitoring system

    International Nuclear Information System (INIS)

    Suryadi; Puranto, Prabowo; Adinanta, Hendra; Tohari, Adrin; Priambodo, Purnomo S

    2017-01-01

    A wireless sensor network has been developed to monitor soil movement of some observed areas periodically. The system consists of four nodes and one gateway which installed on a scope area of 0.2 Km 2 . Each of nodehastwo types of sensor,an inclinometer and an extensometer. An inclinometer sensor is used to measure the tilt of a structure while anextensometer sensor is used to measure the displacement of soil movement. Each of nodeisalso supported by awireless communication device, a solar power supply unit, and a microcontroller unit called sensor module. In this system, there is also gateway module as a main communication system consistinga wireless communication device, power supply unit, and rain gauge to measure the rainfall intensity of the observed area. Each sensor of inclinometer and extensometer isconnected to the sensor module in wiring system but sensor module iscommunicating with gateway in a wireless system. Those four nodes are alsoconnectedeach other in a wireless system collecting the data from inclinometer and extensometer sensors. Module Gateway istransmitting the instruction code to each sensor module one by one and collecting the data from them. Gateway module is an important part to communicate with not only sensor modules but also to the server. This wireless system wasdesigned toreducethe electric consumption powered by 80 WP solar panel and 55Ah battery. This system has been implemented in Pangalengan, Bandung, which has high intensity of rainfall and it can be seen on the website. (paper)

  1. Two-component scattering model and the electron density spectrum

    Science.gov (United States)

    Zhou, A. Z.; Tan, J. Y.; Esamdin, A.; Wu, X. J.

    2010-02-01

    In this paper, we discuss a rigorous treatment of the refractive scintillation caused by a two-component interstellar scattering medium and a Kolmogorov form of density spectrum. It is assumed that the interstellar scattering medium is composed of a thin-screen interstellar medium (ISM) and an extended interstellar medium. We consider the case that the scattering of the thin screen concentrates in a thin layer represented by a δ function distribution and that the scattering density of the extended irregular medium satisfies the Gaussian distribution. We investigate and develop equations for the flux density structure function corresponding to this two-component ISM geometry in the scattering density distribution and compare our result with the observations. We conclude that the refractive scintillation caused by this two-component ISM scattering gives a more satisfactory explanation for the observed flux density variation than does the single extended medium model. The level of refractive scintillation is strongly sensitive to the distribution of scattering material along the line of sight (LOS). The theoretical modulation indices are comparatively less sensitive to the scattering strength of the thin-screen medium, but they critically depend on the distance from the observer to the thin screen. The logarithmic slope of the structure function is sensitive to the scattering strength of the thin-screen medium, but is relatively insensitive to the thin-screen location. Therefore, the proposed model can be applied to interpret the structure functions of flux density observed in pulsar PSR B2111 + 46 and PSR B0136 + 57. The result suggests that the medium consists of a discontinuous distribution of plasma turbulence embedded in the interstellar medium. Thus our work provides some insight into the distribution of the scattering along the LOS to the pulsar PSR B2111 + 46 and PSR B0136 + 57.

  2. A two-component copula with links to insurance

    Directory of Open Access Journals (Sweden)

    Ismail S.

    2017-12-01

    Full Text Available This paper presents a new copula to model dependencies between insurance entities, by considering how insurance entities are affected by both macro and micro factors. The model used to build the copula assumes that the insurance losses of two companies or lines of business are related through a random common loss factor which is then multiplied by an individual random company factor to get the total loss amounts. The new two-component copula is not Archimedean and it extends the toolkit of copulas for the insurance industry.

  3. Vortex formation in a rotating two-component Fermi gas

    Energy Technology Data Exchange (ETDEWEB)

    Warringa, Harmen J.; Sedrakian, Armen [Institut fuer Theoretische Physik, Goethe-Universitaet Frankfurt am Main, Max-von-Laue-Strasse 1, D-60438 Frankfurt am Main (Germany)

    2011-08-15

    A two-component Fermi gas with attractive s-wave interactions forms a superfluid at low temperatures. When this gas is confined in a rotating trap, fermions can unpair at the edges of the gas and vortices can arise beyond certain critical rotation frequencies. We compute these critical rotation frequencies and construct the phase diagram in the plane of scattering length and rotation frequency for different total numbers of particles. We work at zero temperature and consider a cylindrically symmetric harmonic trapping potential. The calculations are performed in the Hartree-Fock-Bogoliubov approximation which implies that our results are quantitatively reliable for weak interactions.

  4. Two component micro injection molding for MID fabrication

    DEFF Research Database (Denmark)

    Islam, Mohammad Aminul; Hansen, Hans Nørgaard; Tang, Peter Torben

    2009-01-01

    Molded Interconnect Devices (MIDs) are plastic substrates with electrical infrastructure. The fabrication of MIDs is usually based on injection molding and different process chains may be identified from this starting point. The use of MIDs has been driven primarily by the automotive sector......, but recently the medical sector seems more and more interested. In particular the possibility of miniaturization of 3D components with electrical infrastructure is attractive. The paper describes possible manufacturing routes and challenges of miniaturized MIDs based on two component micro injection molding...

  5. The chemistry of two-component fluoride crystalline optical media for heavy, fast, radiation hard scintillators

    International Nuclear Information System (INIS)

    Sobolev, B.P.; Krivandina, E.A.; Fedorov, P.P.; Vasilchenko, V.G.

    1994-01-01

    Prospects for preparation of two-component dense optical materials for scintillators are shown, using data on phase diagrams of about 300 MF m - RF n (m, n ≤ 4) type systems, formed by metal fluorides. Primary characteristics (decay time and light output of luminescence, radiation hardness, etc.) of some multicomponent crystals are reported

  6. Urinary incontinence monitoring system using laser-induced graphene sensors

    KAUST Repository

    Nag, Anindya

    2017-12-25

    This paper presents the design and development of a sensor patch to be used in a sensing system to deal with the urinary incontinence problem primarily faced by women and elderly people. The sensor patches were developed from laser-induced graphene from low-cost commercial polyimide (PI) polymers. The graphene was manually transferred to a commercial tape, which was used as sensor patch for experimentation. Salt solutions with different concentrations were tested to determine the most sensitive frequency region of the sensor. The results are encouraging to further develop this sensor in a platform for a fully functional urinary incontinence detection system.

  7. Development of sensor system for indoor location based service implementation

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Joo Heon; Lee, Kyung Ho [Kookmin Univ., Seoul (Korea, Republic of)

    2012-11-15

    This paper introduces a sensor system based on indoor locations in order to implement the Building Energy Management System. This system consists of a thermopile sensor and an ultrasonic sensor. The sensor module is rotated by 360 .deg. and yawed up and down by two electric motors. Therefore, it can simultaneously detect the number and location of the inhabitants in the room. It uses wireless technology to communicate with the building manager or the smart home server, and it can save electric energy by controlling the lighting system or heating/air conditioning equipment automatically. We also demonstrate the usefulness of the proposed system by applying it to a real environment.

  8. Development of sensor system for indoor location based service implementation

    International Nuclear Information System (INIS)

    Cha, Joo Heon; Lee, Kyung Ho

    2012-01-01

    This paper introduces a sensor system based on indoor locations in order to implement the Building Energy Management System. This system consists of a thermopile sensor and an ultrasonic sensor. The sensor module is rotated by 360 .deg. and yawed up and down by two electric motors. Therefore, it can simultaneously detect the number and location of the inhabitants in the room. It uses wireless technology to communicate with the building manager or the smart home server, and it can save electric energy by controlling the lighting system or heating/air conditioning equipment automatically. We also demonstrate the usefulness of the proposed system by applying it to a real environment

  9. Generic Sensor Failure Modeling for Cooperative Systems

    Science.gov (United States)

    Jäger, Georg; Zug, Sebastian

    2018-01-01

    The advent of cooperative systems entails a dynamic composition of their components. As this contrasts current, statically composed systems, new approaches for maintaining their safety are required. In that endeavor, we propose an integration step that evaluates the failure model of shared information in relation to an application’s fault tolerance and thereby promises maintainability of such system’s safety. However, it also poses new requirements on failure models, which are not fulfilled by state-of-the-art approaches. Consequently, this work presents a mathematically defined generic failure model as well as a processing chain for automatically extracting such failure models from empirical data. By examining data of an Sharp GP2D12 distance sensor, we show that the generic failure model not only fulfills the predefined requirements, but also models failure characteristics appropriately when compared to traditional techniques. PMID:29558435

  10. Wireless Sensor Network Metrics for Real-Time Systems

    Science.gov (United States)

    2009-05-20

    Wireless Sensor Network Metrics for Real-Time Systems Phoebus Wei-Chih Chen Electrical Engineering and Computer Sciences University of California at...3. DATES COVERED 00-00-2009 to 00-00-2009 4. TITLE AND SUBTITLE Wireless Sensor Network Metrics for Real-Time Systems 5a. CONTRACT NUMBER 5b... wireless sensor networks (WSNs) is moving from studies of WSNs in isolation toward studies where the WSN is treated as a component of a larger system

  11. Efficient implementation of one- and two-component analytical energy gradients in exact two-component theory

    Science.gov (United States)

    Franzke, Yannick J.; Middendorf, Nils; Weigend, Florian

    2018-03-01

    We present an efficient algorithm for one- and two-component analytical energy gradients with respect to nuclear displacements in the exact two-component decoupling approach to the one-electron Dirac equation (X2C). Our approach is a generalization of the spin-free ansatz by Cheng and Gauss [J. Chem. Phys. 135, 084114 (2011)], where the perturbed one-electron Hamiltonian is calculated by solving a first-order response equation. Computational costs are drastically reduced by applying the diagonal local approximation to the unitary decoupling transformation (DLU) [D. Peng and M. Reiher, J. Chem. Phys. 136, 244108 (2012)] to the X2C Hamiltonian. The introduced error is found to be almost negligible as the mean absolute error of the optimized structures amounts to only 0.01 pm. Our implementation in TURBOMOLE is also available within the finite nucleus model based on a Gaussian charge distribution. For a X2C/DLU gradient calculation, computational effort scales cubically with the molecular size, while storage increases quadratically. The efficiency is demonstrated in calculations of large silver clusters and organometallic iridium complexes.

  12. How insects overcome two-component plant chemical defence

    DEFF Research Database (Denmark)

    Pentzold, Stefan; Zagrobelny, Mika; Rook, Frederik

    2014-01-01

    Insect herbivory is often restricted by glucosylated plant chemical defence compounds that are activated by plant β-glucosidases to release toxic aglucones upon plant tissue damage. Such two-component plant defences are widespread in the plant kingdom and examples of these classes of compounds...... are alkaloid, benzoxazinoid, cyanogenic and iridoid glucosides as well as glucosinolates and salicinoids. Conversely, many insects have evolved a diversity of counteradaptations to overcome this type of constitutive chemical defence. Here we discuss that such counter-adaptations occur at different time points......, before and during feeding as well as during digestion, and at several levels such as the insects’ feeding behaviour, physiology and metabolism. Insect adaptations frequently circumvent or counteract the activity of the plant β-glucosidases, bioactivating enzymes that are a key element in the plant’s two...

  13. Bond strength of two component injection moulded MID

    DEFF Research Database (Denmark)

    Islam, Mohammad Aminul; Hansen, Hans Nørgaard; Tang, Peter Torben

    2006-01-01

    Most products of the future will require industrially adapted, cost effective production processes and on this issue two-component (2K) injection moulding is a potential candidate for MID manufacturing. MID based on 2k injection moulded plastic part with selectively metallised circuit tracks allows...... the two different plastic materials in the MID structure require good bonding between them. This paper finds suitable combinations of materials for MIDs from both bond strength and metallisation view-point. Plastic parts were made by two-shot injection moulding and the effects of some important process...... the integration of electrical and mechanical functionalities in a real 3D structure. If 2k injection moulding is applied with two polymers, of which one is plateable and the other is not, it will be possible to make 3D electrical structures directly on the component. To be applicable in the real engineering field...

  14. Two Component Injection Moulding for Moulded Interconnect Devices

    DEFF Research Database (Denmark)

    Islam, Aminul

    component (2k) injection moulding is one of the most industrially adaptive processes. However, the use of two component injection moulding for MID fabrication, with circuit patterns in sub-millimeter range, is still a big challenge. This book searches for the technical difficulties associated...... with the process and makes attempts to overcome those challenges. In search of suitable polymer materials for MID applications, potential materials are characterized in terms of polymer-polymer bond strength, polymer-polymer interface quality and selective metallization. The experimental results find the factors...... which can effectively control the quality of 2k moulded parts and metallized MIDs. This book presents documented knowledge about MID process chains, 2k moulding and selective metallization which can be valuable source of information for both academic and industrial users....

  15. Chemical evolution of two-component galaxies. II

    International Nuclear Information System (INIS)

    Caimmi, R.

    1978-01-01

    In order to confirm and refine the results obtained in a previous paper the chemical evolution of two-component (spheroid + disk) galaxies is derived rejecting the instantaneous recycling approximation, by means of numerical computations, accounting for (i) the collapse phase of the gas, assumed to be uniform in density and composition, and (ii) a birth-rate stellar function. Computations are performed relatively to the solar neighbourhood and to model galaxies which closely resemble the real morphological sequence: in both cases, numerical results are compared with analytical ones. The numerical models of this paper constitute a first-order approximation, while higher order approximations could be made by rejecting the hypothesis of uniform density and composition, and making use of detailed dynamical models. (Auth.)

  16. Two-component multistep direct reactions: A microscopic approach

    International Nuclear Information System (INIS)

    Koning, A.J.; Chadwick, M.B.

    1998-03-01

    The authors present two principal advances in multistep direct theory: (1) A two-component formulation of multistep direct reactions, where neutron and proton excitations are explicitly accounted for in the evolution of the reaction, for all orders of scattering. While this may at first seem to be a formidable task, especially for multistep processes where the many possible reaction pathways becomes large in a two-component formalism, the authors show that this is not so -- a rather simple generalization of the FKK convolution expression 1 automatically generates these pathways. Such considerations are particularly relevant when simultaneously analyzing both neutron and proton emission spectra, which is always important since these processes represent competing decay channels. (2) A new, and fully microscopic, method for calculating MSD cross sections which does not make use of particle-hole state densities but instead directly calculates cross sections for all possible particle-hole excitations (again including an exact book-keeping of the neutron/proton type of the particle and hole at all stages of the reaction) determined from a simple non-interacting shell model. This is in contrast to all previous numerical approaches which sample only a small number of such states to estimate the DWBA strength, and utilize simple analytical formulae for the partial state density, based on the equidistant spacing model. The new approach has been applied, along with theories for multistep compound, compound, and collective reactions, to analyze experimental emission spectra for a range of targets and energies. The authors show that the theory correctly accounts for double-differential nucleon spectra

  17. Miniaturized, low power FGMOSFET radiation sensor and wireless dosimeter system

    KAUST Repository

    Arsalan, Muhammad

    2013-08-27

    A miniaturized floating gate (FG) MOSFET radiation sensor system is disclosed, The sensor preferably comprises a matched pair of sensor and reference FGMOSFETs wherein the sensor FGMOSFET has a larger area floating gate with an extension over a field oxide layer, for accumulation of charge and increased sensitivity. Elimination of a conventional control gate and injector gate reduces capacitance, and increases sensitivity, and allows for fabrication using standard low cost CMOS technology. A sensor system may be provided with integrated signal processing electronics, for monitoring a change in differential channel current I.sub.D, indicative of radiation dose, and an integrated negative bias generator for automatic pre-charging from a low voltage power source. Optionally, the system may be coupled to a wireless transmitter. A compact wireless sensor System on Package solution is presented, suitable for dosimetry for radiotherapy or other biomedical applications.

  18. Miniaturized, low power FGMOSFET radiation sensor and wireless dosimeter system

    KAUST Repository

    Arsalan, Muhammad; Shamim, Atif; Tarr, Nicholas Garry; Roy, Langis

    2013-01-01

    A miniaturized floating gate (FG) MOSFET radiation sensor system is disclosed, The sensor preferably comprises a matched pair of sensor and reference FGMOSFETs wherein the sensor FGMOSFET has a larger area floating gate with an extension over a field oxide layer, for accumulation of charge and increased sensitivity. Elimination of a conventional control gate and injector gate reduces capacitance, and increases sensitivity, and allows for fabrication using standard low cost CMOS technology. A sensor system may be provided with integrated signal processing electronics, for monitoring a change in differential channel current I.sub.D, indicative of radiation dose, and an integrated negative bias generator for automatic pre-charging from a low voltage power source. Optionally, the system may be coupled to a wireless transmitter. A compact wireless sensor System on Package solution is presented, suitable for dosimetry for radiotherapy or other biomedical applications.

  19. Multi-sensor radiation detector system

    International Nuclear Information System (INIS)

    Foster, R.G.; Cyboron, R.D.

    1975-01-01

    The invention is a multi-sensor radiation detection system including a self-powered detector and an ion or fission chamber, preferably joined as a unitary structure, for removable insertion into a nuclear reactor. The detector and chamber are connected electrically in parallel, requiring but two conductors extending out of the reactor to external electrical circuitry which includes a load impedance, a voltage source, and switch means. The switch means are employed to alternately connect the detector and chamber either with th load impedance or with the load impedance and the voltage source. In the former orientation, current through the load impedance indicates flux intensity at the self-powered detector and in the latter orientation, the current indicates flux intensity at the detector and fission chamber, though almost all of the current is contributed by the fission chamber. (auth)

  20. Sensor systems for the Altair Lunar Lander:

    Energy Technology Data Exchange (ETDEWEB)

    Mariella, R

    2009-12-22

    The Altair Lunar Lander will enable astronauts to learn to live and work on the moon for extended periods of time, providing the experience needed to expand human exploration farther into the solar system. My overriding recommendation: Use independent and complementary [sometimes referred to as 'orthogonal'] techniques to disambiguate confounding/interfering signals. E.g.: a mass spectrometer ['MS'], which currently serves as a Majority Constituent Analyzer ['MCA'] can be very valuable in detecting the presence of a gaseous specie, so long as it falls on a mass-to-charge ratio ['m/z'] that is not already occupied by a majority constituent of cabin air. Consider the toxic gas, CO. Both N{sub 2} and CO have parent peaks of m/z = 28, and CO{sub 2} has a fragment peak at m/z = 28 [and at 16 and 12], so the N{sub 2} and CO{sub 2} m/z=28 signals could mask low, but potentially-dangerous levels of CO. However there are numerous surface-sensitive CO detectors, as well as tunable-diode-laser-based CO sensors that could provide independent monitoring of CO. Also, by appending a gas chromatograph ['GC'] as the front-end sample processer, prior to the inlet of the MS, one can rely upon the GC to separate CO from N{sub 2} and CO{sub 2}, providing the crew with another CO monitor. If the Altair Lunar Lander is able to include a Raman-based MCA for N{sub 2}, O{sub 2}, H{sub 2}O, and CO{sub 2}, then each type of MCA would have cross-references, providing more confidence in the ongoing performance of each technique, and decreasing the risk that one instrument might fail to perform properly, without being noticed. See, also Dr. Pete Snyder's work, which states 'An orthogonal technologies sensor system appears to be attractive for a high confidence detection of presence and temporal characterization of bioaerosols.' Another recommendation: Use data fusion for event detection to decrease uncertainty: tie together the

  1. Advanced interfacing techniques for sensors measurement circuits and systems for intelligent sensors

    CERN Document Server

    Roy, Joyanta; Kumar, V; Mukhopadhyay, Subhas

    2017-01-01

    This book presents ways of interfacing sensors to the digital world, and discusses the marriage between sensor systems and the IoT: the opportunities and challenges. As sensor output is often affected by noise and interference, the book presents effective schemes for recovering the data from a signal that is buried in noise. It also explores interesting applications in the area of health care, un-obstructive monitoring and the electronic nose and tongue. It is a valuable resource for engineers and scientists in the area of sensors and interfacing wanting to update their knowledge of the latest developments in the field and learn more about sensing applications and challenges.

  2. Development of an equipment diagnostic system that evaluates sensor drift

    International Nuclear Information System (INIS)

    Kanada, Masaki; Arita, Setsuo; Tada, Nobuo; Yokota, Katsuo

    2011-01-01

    The importance of condition monitoring technology for equipment has increased with the introduction of condition-based maintenance in nuclear power plants. We are developing a diagnostic system using process signals for plant equipment, such as pumps and motors. It is important to enable the diagnostic system to distinguish sensor drift and equipment failure. We have developed a sensor drift diagnostic method that combines some highly correlative sensor signals by using the MT (Mahalanobis-Taguchi) method. Furthermore, we have developed an equipment failure diagnostic method that measures the Mahalanobis distance from the normal state of equipment by the MT method. These methods can respectively detect sensor drift and equipment failure, but there are the following problems. In the sensor drift diagnosis, there is a possibility of misjudging the sensor drift when the equipment failure occurs and the process signal changes because the behavior of the process signal is the same as that of the sensor drift. Oppositely, in the equipment failure diagnosis, there is a possibility of misjudging the equipment failure when the sensor drift occurs because the sensor drift influences the change of process signal. To solve these problems, we propose a diagnostic method combining the sensor drift diagnosis and the equipment failure diagnosis by the MT method. Firstly, the sensor drift values are estimated by the sensor drift diagnosis, and the sensor drift is removed from the process signal. It is necessary to judge the validity of the estimated sensor drift values before removing the sensor drift from the process signal. We developed a method for judging the validity of the estimated sensor drift values by using the drift distribution based on the sensor calibration data. And then, the equipment failure is diagnosed by using the process signals after removal of the sensor drifts. To verify the developed diagnostic system, several sets of simulation data based on abnormal cases

  3. Energy storage management system with distributed wireless sensors

    Science.gov (United States)

    Farmer, Joseph C.; Bandhauer, Todd M.

    2015-12-08

    An energy storage system having a multiple different types of energy storage and conversion devices. Each device is equipped with one or more sensors and RFID tags to communicate sensor information wirelessly to a central electronic management system, which is used to control the operation of each device. Each device can have multiple RFID tags and sensor types. Several energy storage and conversion devices can be combined.

  4. Density profiles and collective excitations of a trapped two-component Fermi vapour

    International Nuclear Information System (INIS)

    Amoruso, M.; Meccoli, I.; Minguzzi, A.; Tosi, M.P.

    1999-08-01

    We discuss the ground state and the small-amplitude excitations of a degenerate vapour of fermionic atoms placed in two hyperfine states inside a spherical harmonic trap. An equations-of-motion approach is set up to discuss the hydrodynamic dissipation processes from the interactions between the two components of the fluid beyond mean-field theory and to emphasize analogies with spin dynamics and spin diffusion in a homogeneous Fermi liquid. The conditions for the establishment of a collisional regime via scattering against cold-atom impurities are analyzed. The equilibrium density profiles are then calculated for a two-component vapour of 40 K atoms: they are little modified by the interactions for presently relevant values of the system parameters, but spatial separation of the two components will spontaneously arise as the number of atoms in the trap is increased. The eigenmodes of collective oscillation in both the total particle number density and the concentration density are evaluated analytically in the special case of a symmetric two-component vapour in the collisional regime. The dispersion relation of the surface modes for the total particle density reduces in this case to that of a one-component Fermi vapour, whereas the frequencies of all other modes are shifted by the interactions. (author)

  5. Air to fuel ratio sensor for internal combustion engine control system; Nainen kikan no nensho seigyoyo kunen hi sensor

    Energy Technology Data Exchange (ETDEWEB)

    Tsuzuki, M.; Kawai, T.; Yamada, T.; Nishio [NGK Spark Plug Co. Ltd., Aichi (Japan)

    1998-06-01

    Air to fuel ratio sensor is used for emission control system of three-way catalyst, and constitutes the important functional part of combustion control system. For further precise combustion control application, universal air to fuel ratio heated exhaust gas oxygen sensor (UEGO sensor) has been developed. This paper introduces heater control system for constant element temperature of UEGO sensor. By the heater wattage feedback control of sensing cell impedance, the change of sensor element temperature is decreased. 9 refs., 13 figs.

  6. A Tactile Sensor Network System Using a Multiple Sensor Platform with a Dedicated CMOS-LSI for Robot Applications.

    Science.gov (United States)

    Shao, Chenzhong; Tanaka, Shuji; Nakayama, Takahiro; Hata, Yoshiyuki; Bartley, Travis; Nonomura, Yutaka; Muroyama, Masanori

    2017-08-28

    Robot tactile sensation can enhance human-robot communication in terms of safety, reliability and accuracy. The final goal of our project is to widely cover a robot body with a large number of tactile sensors, which has significant advantages such as accurate object recognition, high sensitivity and high redundancy. In this study, we developed a multi-sensor system with dedicated Complementary Metal-Oxide-Semiconductor (CMOS) Large-Scale Integration (LSI) circuit chips (referred to as "sensor platform LSI") as a framework of a serial bus-based tactile sensor network system. The sensor platform LSI supports three types of sensors: an on-chip temperature sensor, off-chip capacitive and resistive tactile sensors, and communicates with a relay node via a bus line. The multi-sensor system was first constructed on a printed circuit board to evaluate basic functions of the sensor platform LSI, such as capacitance-to-digital and resistance-to-digital conversion. Then, two kinds of external sensors, nine sensors in total, were connected to two sensor platform LSIs, and temperature, capacitive and resistive sensing data were acquired simultaneously. Moreover, we fabricated flexible printed circuit cables to demonstrate the multi-sensor system with 15 sensor platform LSIs operating simultaneously, which showed a more realistic implementation in robots. In conclusion, the multi-sensor system with up to 15 sensor platform LSIs on a bus line supporting temperature, capacitive and resistive sensing was successfully demonstrated.

  7. A Tactile Sensor Network System Using a Multiple Sensor Platform with a Dedicated CMOS-LSI for Robot Applications †

    Science.gov (United States)

    Shao, Chenzhong; Tanaka, Shuji; Nakayama, Takahiro; Hata, Yoshiyuki; Bartley, Travis; Muroyama, Masanori

    2017-01-01

    Robot tactile sensation can enhance human–robot communication in terms of safety, reliability and accuracy. The final goal of our project is to widely cover a robot body with a large number of tactile sensors, which has significant advantages such as accurate object recognition, high sensitivity and high redundancy. In this study, we developed a multi-sensor system with dedicated Complementary Metal-Oxide-Semiconductor (CMOS) Large-Scale Integration (LSI) circuit chips (referred to as “sensor platform LSI”) as a framework of a serial bus-based tactile sensor network system. The sensor platform LSI supports three types of sensors: an on-chip temperature sensor, off-chip capacitive and resistive tactile sensors, and communicates with a relay node via a bus line. The multi-sensor system was first constructed on a printed circuit board to evaluate basic functions of the sensor platform LSI, such as capacitance-to-digital and resistance-to-digital conversion. Then, two kinds of external sensors, nine sensors in total, were connected to two sensor platform LSIs, and temperature, capacitive and resistive sensing data were acquired simultaneously. Moreover, we fabricated flexible printed circuit cables to demonstrate the multi-sensor system with 15 sensor platform LSIs operating simultaneously, which showed a more realistic implementation in robots. In conclusion, the multi-sensor system with up to 15 sensor platform LSIs on a bus line supporting temperature, capacitive and resistive sensing was successfully demonstrated. PMID:29061954

  8. Sensor Webs as Virtual Data Systems for Earth Science

    Science.gov (United States)

    Moe, K. L.; Sherwood, R.

    2008-05-01

    The NASA Earth Science Technology Office established a 3-year Advanced Information Systems Technology (AIST) development program in late 2006 to explore the technical challenges associated with integrating sensors, sensor networks, data assimilation and modeling components into virtual data systems called "sensor webs". The AIST sensor web program was initiated in response to a renewed emphasis on the sensor web concepts. In 2004, NASA proposed an Earth science vision for a more robust Earth observing system, coupled with remote sensing data analysis tools and advances in Earth system models. The AIST program is conducting the research and developing components to explore the technology infrastructure that will enable the visionary goals. A working statement for a NASA Earth science sensor web vision is the following: On-demand sensing of a broad array of environmental and ecological phenomena across a wide range of spatial and temporal scales, from a heterogeneous suite of sensors both in-situ and in orbit. Sensor webs will be dynamically organized to collect data, extract information from it, accept input from other sensor / forecast / tasking systems, interact with the environment based on what they detect or are tasked to perform, and communicate observations and results in real time. The focus on sensor webs is to develop the technology and prototypes to demonstrate the evolving sensor web capabilities. There are 35 AIST projects ranging from 1 to 3 years in duration addressing various aspects of sensor webs involving space sensors such as Earth Observing-1, in situ sensor networks such as the southern California earthquake network, and various modeling and forecasting systems. Some of these projects build on proof-of-concept demonstrations of sensor web capabilities like the EO-1 rapid fire response initially implemented in 2003. Other projects simulate future sensor web configurations to evaluate the effectiveness of sensor-model interactions for producing

  9. AMA Conferences 2015. SENSOR 2015. 17th international conference on sensors and measurement technology. IRS2 2015. 14th international conference on infrared sensors and systems. Proceedings

    International Nuclear Information System (INIS)

    2015-01-01

    This meeting paper contains presentations of two conferences: SENSOR 2015 and IRS 2 (= International conference on InfraRed Sensors and systems). The first part of SENSOR 2015 contains the following chapters: (A) SENSOR PRINCIPLES: A.1: Mechanical sensors; A.2: Optical sensors; A.3: Ultrasonic sensors; A.4: Microacoustic sensors; A.5: Magnetic sensors; A.6: Impedance sensors; A.7: Gas sensors; A.8: Flow sensors; A.9: Dimensional measurement; A.10: Temperature and humidity sensors; A.11: Chemosensors; A.12: Biosensors; A.13: Embedded sensors; A.14: Sensor-actuator systems; (B) SENSOR TECHNOLOGY: B.1: Sensor design; B.2: Numerical simulation of sensors; B.3: Sensor materials; B.4: MEMS technology; B.5: Micro-Nano-Integration; B.6: Packaging; B.7: Materials; B.8: Thin films; B.9: Sensor production; B.10: Sensor reliability; B.11: Calibration and testing; B.12: Optical fibre sensors. (C) SENSOR ELECTRONICS AND COMMUNICATION: C.1: Sensor electronics; C.2: Sensor networks; C.3: Wireless sensors; C.4: Sensor communication; C.5: Energy harvesting; C.6: Measuring systems; C.7: Embedded systems; C.8: Self-monitoring and diagnosis; (D) APPLICATIONS: D.1: Medical measuring technology; D.2: Ambient assisted living; D.3: Process measuring technology; D.4: Automotive; D.5: Sensors in energy technology; D.6: Production technology; D.7: Security technology; D.8: Smart home; D.9: Household technology. The second part with the contributions of the IRS 2 2015 is structured as follows: (E) INFRARED SENSORS: E.1: Photon detectors; E.2: Thermal detectors; E.3: Cooled detectors; E.4: Uncooled detectors; E.5: Sensor modules; E.6: Sensor packaging. (G) INFRARED SYSTEMS AND APPLICATIONS: G.1: Thermal imaging; G.2: Pyrometry / contactless temperature measurement; G.3: Gas analysis; G.4: Spectroscopy; G.5: Motion control and presence detection; G.6: Security and safety monitoring; G.7: Non-destructive testing; F: INFRARED SYSTEM COMPONENTS: F.1: Infrared optics; F.2: Optical modulators; F.3

  10. Therapeutic hypertension system based on a microbreathing pressure sensor system

    Directory of Open Access Journals (Sweden)

    Diao Z

    2011-05-01

    Full Text Available Ziji Diao1, Hongying Liu1, Lan Zhu1, Xiaoqiang Gao1, Suwen Zhao1, Xitian Pi1,2, Xiaolin Zheng1,21Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Chongqing; 2Key Laboratories for National Defense Science and Technology of Innovative Micronano Devices and System Technology, Chongqing, People’s Republic of ChinaBackground and methods: A novel therapeutic system for the treatment of hypertension was developed on the basis of a slow-breath training mechanism, using a microbreathing pressure sensor device for the detection of human respiratory signals attached to the abdomen. The system utilizes a single-chip AT89C51 microcomputer as a core processor, programmed by Microsoft Visual C++6.0 to communicate with a PC via a full-speed PDIUSBD12 interface chip. The programming is based on a slow-breath guided algorithm in which the respiratory signal serves as a physiological feedback parameter. Inhalation and exhalation by the subject is guided by music signals.Results and conclusion: Our study indicates that this microbreathing sensor system may assist in slow-breath training and may help to decrease blood pressure.Keywords: hypertension, microbreathing sensor, single-chip microcomputer, slow-pace breathing

  11. A minimal model for two-component dark matter

    International Nuclear Information System (INIS)

    Esch, Sonja; Klasen, Michael; Yaguna, Carlos E.

    2014-01-01

    We propose and study a new minimal model for two-component dark matter. The model contains only three additional fields, one fermion and two scalars, all singlets under the Standard Model gauge group. Two of these fields, one fermion and one scalar, are odd under a Z_2 symmetry that renders them simultaneously stable. Thus, both particles contribute to the observed dark matter density. This model resembles the union of the singlet scalar and the singlet fermionic models but it contains some new features of its own. We analyze in some detail its dark matter phenomenology. Regarding the relic density, the main novelty is the possible annihilation of one dark matter particle into the other, which can affect the predicted relic density in a significant way. Regarding dark matter detection, we identify a new contribution that can lead either to an enhancement or to a suppression of the spin-independent cross section for the scalar dark matter particle. Finally, we define a set of five benchmarks models compatible with all present bounds and examine their direct detection prospects at planned experiments. A generic feature of this model is that both particles give rise to observable signals in 1-ton direct detection experiments. In fact, such experiments will be able to probe even a subdominant dark matter component at the percent level.

  12. Parameter studies for a two-component fusion experiment

    International Nuclear Information System (INIS)

    Towner, H.H.

    1975-01-01

    The sensitivity of the energy multiplication of a two-component fusion experiment is examined relative to the following parameters: energy confinement time (tau/sub E/), particle confinement time (tau/sub p/), effective Z of the plasma (Z/sub eff/), injection rate (j/sub I/) and injection energy (E/sub I/). The Energy Research and Development Administration recently approved funding for such a fusion device (the Toroidal Fusion Test Reactor or TFTR) which will be built at the Princeton Plasma Physics Laboratory. Hence, such a parameter study seems both timely and necessary. This work also serves as an independent check on the design values proposed for the TFTR to enable it to achieve energy breakeven (F = 1). Using the nominal TFTR design parameters and a self-consistent ion-electron power balance, the maximum F-value is found to be approximately 1.2 which occurs at an injection energy of approximately 210 KeV. The injector operation, i.e. its current and energy capability are shown to be a very critical factor in the TFTR performance. However, if the injectors meet the design objectives, there appears to be sufficient latitude in the other parameters to offer reasonable assurance that energy breakeven can be achieved. (U.S.)

  13. Cold component flow in a two-component mirror machine

    International Nuclear Information System (INIS)

    Rognlien, T.D.

    1975-12-01

    Steady-state solutions are given for the flow characteristics along the magnetic field of the cold plasma component in a two-component mirror machine. The hot plasma component is represented by a fixed density profile. The fluid equations are used to describe the cold plasma, which is assumed to be generated in a localized region at one end of the machine. The ion flow speed, v/sub i/, is required to satisfy the Bohm sheath condition at the end walls, i.e., v/sub i/ greater than or equal to c/sub s/, where c/sub s/ is the ion-acoustic speed. For the case when the cold plasma density, n/sub c/, is much less than the hot plasma density, n/sub h/, the cold plasma is stagnant and does not penetrate through the machine in the zero temperature case. The effect of a finite temperature is to allow for the penetration of a small amount of cold plasma through the machine. For the density range n/sub c/ approximately n/sub h/, the flow solutions are asymmetric about the midplane and have v/sub i/ = c/sub s/ near the midplane. Finally, for n/sub c/ much greater than n/sub h/, the solutions become symmetric about the midplane and approach the Lee--McNamara type solutions with v/sub i/ = c/sub s/ near the mirror throats

  14. Fast-wave heating of a two-component plasma

    International Nuclear Information System (INIS)

    Stix, T.H.

    1975-02-01

    The use of the compressional hydromagnetic mode (also called the magnetosonic or, simply, the fast wave) is examined in some detail with respect to the heating of a tritium plasma containing a few percent deuterium. Efficient absorption of wave energy by the deuteron component is found when ω = ω/sub c/ (deuterons), with Q/sub wave/ greater than or equal to 100. The dominant behavior of the high-energy deuteron distribution function is found to be f(v) approximately exp[3/2) ∫/sup v/ dv less than Δv greater than/less than(Δv/sub perpendicular to/) 2 greater than], where [Δv] is the Chandrasekhar-Spitzer drag coefficient, and [(Δv/sub perpendicular to/) 2 sigma] is the Kennel-Englemann quasilinear diffusion coefficient for wave--particle interaction at the deuteron cyclotron frequency. An analytic solution to the one-dimensional Fokker--Planck equation, with rf-induced diffusion, is developed, and using this solution together with Duane's fit to the D-T fusion cross-section, it is found that the nuclear fusion power output from an rf-produced two-component plasma can significantly exceed the incremental (radiofrequency) power input. (auth)

  15. Implementation of two-component advective flow solution in XSPEC

    Science.gov (United States)

    Debnath, Dipak; Chakrabarti, Sandip K.; Mondal, Santanu

    2014-05-01

    Spectral and temporal properties of black hole candidates can be explained reasonably well using Chakrabarti-Titarchuk solution of two-component advective flow (TCAF). This model requires two accretion rates, namely the Keplerian disc accretion rate and the halo accretion rate, the latter being composed of a sub-Keplerian, low-angular-momentum flow which may or may not develop a shock. In this solution, the relevant parameter is the relative importance of the halo (which creates the Compton cloud region) rate with respect to the Keplerian disc rate (soft photon source). Though this model has been used earlier to manually fit data of several black hole candidates quite satisfactorily, for the first time, we made it user friendly by implementing it into XSPEC software of Goddard Space Flight Center (GSFC)/NASA. This enables any user to extract physical parameters of the accretion flows, such as two accretion rates, the shock location, the shock strength, etc., for any black hole candidate. We provide some examples of fitting a few cases using this model. Most importantly, unlike any other model, we show that TCAF is capable of predicting timing properties from the spectral fits, since in TCAF, a shock is responsible for deciding spectral slopes as well as quasi-periodic oscillation frequencies. L86

  16. Light-front QCD. II. Two-component theory

    International Nuclear Information System (INIS)

    Zhang, W.; Harindranath, A.

    1993-01-01

    The light-front gauge A a + =0 is known to be a convenient gauge in practical QCD calculations for short-distance behavior, but there are persistent concerns about its use because of its ''singular'' nature. The study of nonperturbative field theory quantizing on a light-front plane for hadronic bound states requires one to gain a priori systematic control of such gauge singularities. In the second paper of this series we study the two-component old-fashioned perturbation theory and various severe infrared divergences occurring in old-fashioned light-front Hamiltonian calculations for QCD. We also analyze the ultraviolet divergences associated with a large transverse momentum and examine three currently used regulators: an explicit transverse cutoff, transverse dimensional regularization, and a global cutoff. We discuss possible difficulties caused by the light-front gauge singularity in the applications of light-front QCD to both old-fashioned perturbative calculations for short-distance physics and upcoming nonperturbative investigations for hadronic bound states

  17. Economic consequences of investing in sensor systems on dairy farms

    NARCIS (Netherlands)

    Steeneveld, W.; Hogeveen, H.; Oude Lansink, A.G.J.M.

    2015-01-01

    The objective of this study was to investigate the impact of investment in sensor systems on productivity change, using farm accounting data. Farm accounting data for the years 2008–2013 was available for 217 Dutch dairy farms. In addition, information was available on the adoption of sensor systems

  18. Security Techniques for Sensor Systems and the Internet of Things

    Science.gov (United States)

    Midi, Daniele

    2016-01-01

    Sensor systems are becoming pervasive in many domains, and are recently being generalized by the Internet of Things (IoT). This wide deployment, however, presents significant security issues. We develop security techniques for sensor systems and IoT, addressing all security management phases. Prior to deployment, the nodes need to be hardened. We…

  19. Solid-State Gas Sensors: Sensor System Challenges in the Civil Security Domain

    Directory of Open Access Journals (Sweden)

    Gerhard Müller

    2016-01-01

    Full Text Available The detection of military high explosives and illicit drugs presents problems of paramount importance in the fields of counter terrorism and criminal investigation. Effectively dealing with such threats requires hand-portable, mobile and affordable instruments. The paper shows that solid-state gas sensors can contribute to the development of such instruments provided the sensors are incorporated into integrated sensor systems, which acquire the target substances in the form of particle residue from suspect objects and which process the collected residue through a sequence of particle sampling, solid-vapor conversion, vapor detection and signal treatment steps. Considering sensor systems with metal oxide gas sensors at the backend, it is demonstrated that significant gains in sensitivity, selectivity and speed of response can be attained when the threat substances are sampled in particle as opposed to vapor form.

  20. Solid-State Gas Sensors: Sensor System Challenges in the Civil Security Domain.

    Science.gov (United States)

    Müller, Gerhard; Hackner, Angelika; Beer, Sebastian; Göbel, Johann

    2016-01-20

    The detection of military high explosives and illicit drugs presents problems of paramount importance in the fields of counter terrorism and criminal investigation. Effectively dealing with such threats requires hand-portable, mobile and affordable instruments. The paper shows that solid-state gas sensors can contribute to the development of such instruments provided the sensors are incorporated into integrated sensor systems, which acquire the target substances in the form of particle residue from suspect objects and which process the collected residue through a sequence of particle sampling, solid-vapor conversion, vapor detection and signal treatment steps. Considering sensor systems with metal oxide gas sensors at the backend, it is demonstrated that significant gains in sensitivity, selectivity and speed of response can be attained when the threat substances are sampled in particle as opposed to vapor form.

  1. Continuous liquid level monitoring sensor system using fiber Bragg grating

    Science.gov (United States)

    Sengupta, Dipankar; Kishore, Putha

    2014-01-01

    The design and packaging of simple, small, and low cost sensor heads, used for continuous liquid level measurement using uniformly thinned (etched) optical fiber Bragg grating (FBG) are proposed. The sensor system consists of only an FBG and a simple detection system. The sensitivity of sensor is found to be 23 pm/cm of water column pressure. A linear optical fiber edge filter is designed and developed for the conversion of Bragg wavelength shift to its equivalent intensity. The result shows that relative power measured by a photo detector is linearly proportional to the liquid level. The obtained sensitivity of the sensor is nearly -15 mV/cm.

  2. Circuits and Systems for Low-Power Miniaturized Wireless Sensors

    Science.gov (United States)

    Nagaraju, Manohar

    The field of electronic sensors has witnessed a tremendous growth over the last decade particularly with the proliferation of mobile devices. New applications in Internet of Things (IoT), wearable technology, are further expected to fuel the demand for sensors from current numbers in the range of billions to trillions in the next decade. The main challenges for a trillion sensors are continued miniaturization, low-cost and large-scale manufacturing process, and low power consumption. Traditional integration and circuit design techniques in sensor systems are not suitable for applications in smart dust, IoT etc. The first part of this thesis demonstrates an example sensor system for biosignal recording and illustrates the tradeoffs in the design of low-power miniaturized sensors. The different components of the sensor system are integrated at the board level. The second part of the thesis demonstrates fully integrated sensors that enable extreme miniaturization of a sensing system with the sensor element, processing circuitry, a frequency reference for communication and the communication circuitry in a single hermetically sealed die. Design techniques to reduce the power consumption of the sensor interface circuitry at the architecture and circuit level are demonstrated. The principles are used to design sensors for two of the most common physical variables, mass and pressure. A low-power wireless mass and pressure sensor suitable for a wide variety of biological/chemical sensing applications and Tire Pressure Monitoring Systems (TPMS) respectively are demonstrated. Further, the idea of using high-Q resonators for a Voltage Controlled Oscillator (VCO) is proposed and a low-noise, wide bandwidth FBAR-based VCO is presented.

  3. Sensor network based vehicle classification and license plate identification system

    Energy Technology Data Exchange (ETDEWEB)

    Frigo, Janette Rose [Los Alamos National Laboratory; Brennan, Sean M [Los Alamos National Laboratory; Rosten, Edward J [Los Alamos National Laboratory; Raby, Eric Y [Los Alamos National Laboratory; Kulathumani, Vinod K [WEST VIRGINIA UNIV.

    2009-01-01

    Typically, for energy efficiency and scalability purposes, sensor networks have been used in the context of environmental and traffic monitoring applications in which operations at the sensor level are not computationally intensive. But increasingly, sensor network applications require data and compute intensive sensors such video cameras and microphones. In this paper, we describe the design and implementation of two such systems: a vehicle classifier based on acoustic signals and a license plate identification system using a camera. The systems are implemented in an energy-efficient manner to the extent possible using commercially available hardware, the Mica motes and the Stargate platform. Our experience in designing these systems leads us to consider an alternate more flexible, modular, low-power mote architecture that uses a combination of FPGAs, specialized embedded processing units and sensor data acquisition systems.

  4. Underwater Animal Monitoring Magnetic Sensor System

    KAUST Repository

    Kaidarova, Altynay

    2017-01-01

    solid-state sensors today, coupled with flexible magnetic composites. The TMR sensors are composed of CoFeB free layers and MgO tunnel barriers, patterned using standard optical lithography and ion milling procedures. The short and long-term stability

  5. Self-powered optical sensor systems

    NARCIS (Netherlands)

    Wu, H.; Emadi, A.; Graaf, G. de; Leijtens, J.A.P.; Wolffenbuttel, R.F.

    2009-01-01

    A 0.35 μm CMOS process has been used for on-chip integration of a sun sensor composed of a 2x2 photodiode array and a current-to-voltage amplifier. Unlike conventional sun sensors, a shade profile proportional to the angle of incidence of incoming light is projected onto the photodiodes. This

  6. A portable readout system for silicon microstrip sensors

    International Nuclear Information System (INIS)

    Marco-Hernandez, Ricardo

    2010-01-01

    This system can measure the collected charge in one or two microstrip silicon sensors by reading out all the channels of the sensor(s), up to 256. The system is able to operate with different types (p- and n-type) and different sizes (up to 3 cm 2 ) of microstrip silicon sensors, both irradiated and non-irradiated. Heavily irradiated sensors will be used at the Super Large Hadron Collider, so this system can be used to research the performance of microstrip silicon sensors in conditions as similar as possible to the Super Large Hadron Collider operating conditions. The system has two main parts: a hardware part and a software part. The hardware part acquires the sensor signals either from external trigger inputs, in case of a radioactive source setup is used, or from a synchronised trigger output generated by the system, if a laser setup is used. The software controls the system and processes the data acquired from the sensors in order to store it in an adequate format. The main characteristics of the system are described. Results of measurements acquired with n- and p-type detectors using both the laser and the radioactive source setup are also presented and discussed.

  7. An expert system for sensor data validation and malfunction detection

    International Nuclear Information System (INIS)

    Hashemi, S.; Hajek, B.K.; Miller, D.W.; Chandrasekaran, B.; Punch, W.F. III.

    1987-01-01

    During recent years, applications of expert systems in different fields of engineering have been under study throughout the world. At the Ohio State University, the theories developed by the Laboratory for Artificial Intelligence Research (LAIR) have been implemented for nuclear power plants and chemical processing systems. For nuclear power plants, these techniques have been further developed to reach diagnostic conclusions about malfunctions and faulty sensors, as well as to suggest corrective actions about the malfunctions. This paper concentrates on the AI applications to plant diagnosis and faulty sensor identifications. To achieve the above goals without adding extra sensors in a plant, the use of unlike sensor data (such as relationships between pressure and temperature in a Boiling Water Reactor (BWR)) and diagnostic conclusions about malfunctions as backups for suspicious sensors has been made. This extra evidence is readily available throughout the plant and is not generally used to backup suspicious sensor data in any manner

  8. Competitive adsorption of a two-component gas on a deformable adsorbent

    International Nuclear Information System (INIS)

    Usenko, A S

    2014-01-01

    We investigate the competitive adsorption of a two-component gas on the surface of an adsorbent whose adsorption properties vary due to the adsorbent deformation. The essential difference of adsorption isotherms for a deformable adsorbent both from the classical Langmuir adsorption isotherms of a two-component gas and from the adsorption isotherms of a one-component gas is obtained, taking into account variations in the adsorption properties of the adsorbent in adsorption. We establish bistability and tristability of the system caused by variations in adsorption properties of the adsorbent in competitive adsorption of gas particles on it. We derive conditions under which adsorption isotherms of a binary gas mixture have two stable asymptotes. It is shown that the specific features of the behavior of the system under study can be described in terms of a potential of the known explicit form. (paper)

  9. The Radio Frequency Health Node Wireless Sensor System

    Science.gov (United States)

    Valencia, J. Emilio; Stanley, Priscilla C.; Mackey, Paul J.

    2009-01-01

    The Radio Frequency Health Node (RFHN) wireless sensor system differs from other wireless sensor systems in ways originally intended to enhance utility as an instrumentation system for a spacecraft. The RFHN can also be adapted to use in terrestrial applications in which there are requirements for operational flexibility and integrability into higher-level instrumentation and data acquisition systems. As shown in the figure, the heart of the system is the RFHN, which is a unit that passes commands and data between (1) one or more commercially available wireless sensor units (optionally, also including wired sensor units) and (2) command and data interfaces with a local control computer that may be part of the spacecraft or other engineering system in which the wireless sensor system is installed. In turn, the local control computer can be in radio or wire communication with a remote control computer that may be part of a higher-level system. The remote control computer, acting via the local control computer and the RFHN, cannot only monitor readout data from the sensor units but can also remotely configure (program or reprogram) the RFHN and the sensor units during operation. In a spacecraft application, the RFHN and the sensor units can also be configured more nearly directly, prior to launch, via a serial interface that includes an umbilical cable between the spacecraft and ground support equipment. In either case, the RFHN wireless sensor system has the flexibility to be configured, as required, with different numbers and types of sensors for different applications. The RFHN can be used to effect realtime transfer of data from, and commands to, the wireless sensor units. It can also store data for later retrieval by an external computer. The RFHN communicates with the wireless sensor units via a radio transceiver module. The modular design of the RFHN makes it possible to add radio transceiver modules as needed to accommodate additional sets of wireless sensor

  10. The two-component afterglow of Swift GRB 050802

    Science.gov (United States)

    Oates, S. R.; de Pasquale, M.; Page, M. J.; Blustin, A. J.; Zane, S.; McGowan, K.; Mason, K. O.; Poole, T. S.; Schady, P.; Roming, P. W. A.; Page, K. L.; Falcone, A.; Gehrels, N.

    2007-09-01

    This paper investigates GRB 050802, one of the best examples of a Swift gamma-ray burst afterglow that shows a break in the X-ray light curve, while the optical counterpart decays as a single power law. This burst has an optically bright afterglow of 16.5 mag, detected throughout the 170-650nm spectral range of the Ultraviolet and Optical Telescope (UVOT) onboard Swift. Observations began with the X-ray Telescope and UVOT telescopes 286s after the initial trigger and continued for 1.2 ×106s. The X-ray light curve consists of three power-law segments: a rise until 420s, followed by a slow decay with α =0.63 +/-0.03 until 5000s, after which, the light curve decays faster with a slope of α3 =1.59 +/-0.03. The optical light curve decays as a single power law with αO =0.82 +/-0.03 throughout the observation. The X-ray data on their own are consistent with the break at 5000s being due to the end of energy injection. Modelling the optical to X-ray spectral energy distribution, we find that the optical afterglow cannot be produced by the same component as the X-ray emission at late times, ruling out a single-component afterglow. We therefore considered two-component jet models and find that the X-ray and optical emission is best reproduced by a model in which both components are energy injected for the duration of the observed afterglow and the X-ray break at 5000s is due to a jet break in the narrow component. This bright, well-observed burst is likely a guide for interpreting the surprising finding of Swift that bursts seldom display achromatic jet breaks.

  11. Smart architecture for stable multipoint fiber Bragg grating sensor system

    Science.gov (United States)

    Yeh, Chien-Hung; Tsai, Ning; Zhuang, Yuan-Hong; Huang, Tzu-Jung; Chow, Chi-Wai; Chen, Jing-Heng; Liu, Wen-Fung

    2017-12-01

    In this work, we propose and investigate an intelligent fiber Bragg grating (FBG)-based sensor system in which the proposed stabilized and wavelength-tunable single-longitudinal-mode erbium-doped fiber laser can improve the sensing accuracy of wavelength-division-multiplexing multiple FBG sensors in a longer fiber transmission distance. Moreover, we also demonstrate the proposed sensor architecture to enhance the FBG capacity for sensing strain and temperature, simultaneously.

  12. Dual cameras acquisition and display system of retina-like sensor camera and rectangular sensor camera

    Science.gov (United States)

    Cao, Nan; Cao, Fengmei; Lin, Yabin; Bai, Tingzhu; Song, Shengyu

    2015-04-01

    For a new kind of retina-like senor camera and a traditional rectangular sensor camera, dual cameras acquisition and display system need to be built. We introduce the principle and the development of retina-like senor. Image coordinates transformation and interpolation based on sub-pixel interpolation need to be realized for our retina-like sensor's special pixels distribution. The hardware platform is composed of retina-like senor camera, rectangular sensor camera, image grabber and PC. Combined the MIL and OpenCV library, the software program is composed in VC++ on VS 2010. Experience results show that the system can realizes two cameras' acquisition and display.

  13. Integrated active sensor system for real time vibration monitoring.

    Science.gov (United States)

    Liang, Qijie; Yan, Xiaoqin; Liao, Xinqin; Cao, Shiyao; Lu, Shengnan; Zheng, Xin; Zhang, Yue

    2015-11-05

    We report a self-powered, lightweight and cost-effective active sensor system for vibration monitoring with multiplexed operation based on contact electrification between sensor and detected objects. The as-fabricated sensor matrix is capable of monitoring and mapping the vibration state of large amounts of units. The monitoring contents include: on-off state, vibration frequency and vibration amplitude of each unit. The active sensor system delivers a detection range of 0-60 Hz, high accuracy (relative error below 0.42%), long-term stability (10000 cycles). On the time dimension, the sensor can provide the vibration process memory by recording the outputs of the sensor system in an extend period of time. Besides, the developed sensor system can realize detection under contact mode and non-contact mode. Its high performance is not sensitive to the shape or the conductivity of the detected object. With these features, the active sensor system has great potential in automatic control, remote operation, surveillance and security systems.

  14. MicroSensors Systems: detection of a dismounted threat

    Science.gov (United States)

    Davis, Bill; Berglund, Victor; Falkofske, Dwight; Krantz, Brian

    2005-05-01

    The Micro Sensor System (MSS) is a layered sensor network with the goal of detecting dismounted threats approaching high value assets. A low power unattended ground sensor network is dependant on a network protocol for efficiency in order to minimize data transmissions after network establishment. The reduction of network 'chattiness' is a primary driver for minimizing power consumption and is a factor in establishing a low probability of detection and interception. The MSS has developed a unique protocol to meet these challenges. Unattended ground sensor systems are most likely dependant on batteries for power which due to size determines the ability of the sensor to be concealed after placement. To minimize power requirements, overcome size limitations, and maintain a low system cost the MSS utilizes advanced manufacturing processes know as Fluidic Self-Assembly and Chip Scale Packaging. The type of sensing element and the ability to sense various phenomenologies (particularly magnetic) at ranges greater than a few meters limits the effectiveness of a system. The MicroSensor System will overcome these limitations by deploying large numbers of low cost sensors, which is made possible by the advanced manufacturing process used in production of the sensors. The MSS program will provide unprecedented levels of real-time battlefield information which greatly enhances combat situational awareness when integrated with the existing Command, Control, Communications, Computers, Intelligence, Surveillance and Reconnaissance (C4ISR) infrastructure. This system will provide an important boost to realizing the information dominant, network-centric objective of Joint Vision 2020.

  15. SENSORS FAULT DIAGNOSIS ALGORITHM DESIGN OF A HYDRAULIC SYSTEM

    Directory of Open Access Journals (Sweden)

    Matej ORAVEC

    2017-06-01

    Full Text Available This article presents the sensors fault diagnosis system design for the hydraulic system, which is based on the group of the three fault estimation filters. These filters are used for estimation of the system states and sensors fault magnitude. Also, this article briefly stated the hydraulic system state control design with integrator, which is important assumption for the fault diagnosis system design. The sensors fault diagnosis system is implemented into the Matlab/Simulink environment and it is verified using the controlled hydraulic system simulation model. Verification of the designed fault diagnosis system is realized by series of experiments, which simulates sensors faults. The results of the experiments are briefly presented in the last part of this article.

  16. Remote Laser Evaporative Molecular Absorption Spectroscopy Sensor System

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose a sensor system capable of remotely probing the molecular composition of cold solar system targets (asteroids, comets, planets, moons), such as from a...

  17. A Wildlife Monitoring System Based on Wireless Image Sensor Networks

    Directory of Open Access Journals (Sweden)

    Junguo Zhang

    2014-10-01

    Full Text Available Survival and development of wildlife sustains the balance and stability of the entire ecosystem. Wildlife monitoring can provide lots of information such as wildlife species, quantity, habits, quality of life and habitat conditions, to help researchers grasp the status and dynamics of wildlife resources, and to provide basis for the effective protection, sustainable use, and scientific management of wildlife resources. Wildlife monitoring is the foundation of wildlife protection and management. Wireless Sensor Networks (WSN technology has become the most popular technology in the field of information. With advance of the CMOS image sensor technology, wireless sensor networks combined with image sensors, namely Wireless Image Sensor Networks (WISN technology, has emerged as an alternative in monitoring applications. Monitoring wildlife is one of its most promising applications. In this paper, system architecture of the wildlife monitoring system based on the wireless image sensor networks was presented to overcome the shortcomings of the traditional monitoring methods. Specifically, some key issues including design of wireless image sensor nodes and software process design have been studied and presented. A self-powered rotatable wireless infrared image sensor node based on ARM and an aggregation node designed for large amounts of data were developed. In addition, their corresponding software was designed. The proposed system is able to monitor wildlife accurately, automatically, and remotely in all-weather condition, which lays foundations for applications of wireless image sensor networks in wildlife monitoring.

  18. Development of a wireless MEMS multifunction sensor system and field demonstration of embedded sensors for monitoring concrete pavements, volume II

    Science.gov (United States)

    2016-08-01

    This two-pronged study evaluated the performance of commercial off-the-shelf (COTS) micro-electromechanical sensors and systems (MEMS) embedded in concrete pavement (Final Report Volume I) and developed a wireless MEMS multifunctional sensor system f...

  19. Sensor concentrator unit for the Continuous Automated Vault Inventory System

    International Nuclear Information System (INIS)

    Nodine, R.N.; Lenarduzzi, R.

    1997-06-01

    The purpose of this document is to describe the use and operation of the sensor concentrator in the Continuous Automated Vault Inventory System (CAVIS). The CAVIS electronically verifies the presence of items of stored special nuclear material (SNM). US Department of Energy orders require that stored SNM be inventoried periodically to provide assurance that the material is secure. Currently this inventory is a highly manual activity, requiring personnel to enter the storage vaults. Using a CAVIS allows the frequency of physical inventories to be significantly reduced, resulting in substantial cost savings, increased security, and improved safety. The electronic inventory of stored SNM requires two different types of sensors for each item. The two sensors measure different parameters of the item, usually weight and gamma rays. A CAVIS is constructed using four basic system components: sensors, sensor concentrators, a data collection unit, and a database/user interface unit. One sensor concentrator supports the inventory of up to 20 items (40 sensors) and continuously takes readings from the item sensors. On request the sensor concentrator outputs the most recent sensor readings to the data collection unit. The information transfer takes place over a RS485 communications link. The data collection unit supports from 1 to 120 sensor concentrators (1 to 2,400 items) and is referred to as the Sensor Polling and Configuration System (SPCS). The SPCS is connected by a secure Transmission Control Protocol/Internet Protocol (TCP/IP) network to the database/user interface unit, which is referred to as the Graphical Facility Information Center (GraFIC). A CAVIS containing more than 2,400 items is supported by connecting additional SPCS units to the GraFIC

  20. Sensor concentrator unit for the Continuous Automated Vault Inventory System

    Energy Technology Data Exchange (ETDEWEB)

    Nodine, R.N.; Lenarduzzi, R.

    1997-06-01

    The purpose of this document is to describe the use and operation of the sensor concentrator in the Continuous Automated Vault Inventory System (CAVIS). The CAVIS electronically verifies the presence of items of stored special nuclear material (SNM). US Department of Energy orders require that stored SNM be inventoried periodically to provide assurance that the material is secure. Currently this inventory is a highly manual activity, requiring personnel to enter the storage vaults. Using a CAVIS allows the frequency of physical inventories to be significantly reduced, resulting in substantial cost savings, increased security, and improved safety. The electronic inventory of stored SNM requires two different types of sensors for each item. The two sensors measure different parameters of the item, usually weight and gamma rays. A CAVIS is constructed using four basic system components: sensors, sensor concentrators, a data collection unit, and a database/user interface unit. One sensor concentrator supports the inventory of up to 20 items (40 sensors) and continuously takes readings from the item sensors. On request the sensor concentrator outputs the most recent sensor readings to the data collection unit. The information transfer takes place over a RS485 communications link. The data collection unit supports from 1 to 120 sensor concentrators (1 to 2,400 items) and is referred to as the Sensor Polling and Configuration System (SPCS). The SPCS is connected by a secure Transmission Control Protocol/Internet Protocol (TCP/IP) network to the database/user interface unit, which is referred to as the Graphical Facility Information Center (GraFIC). A CAVIS containing more than 2,400 items is supported by connecting additional SPCS units to the GraFIC.

  1. Analysis of LARI sensor system: Final report

    International Nuclear Information System (INIS)

    Pfisterer, R.N.; Peterson, G.L.; Breault, R.P.

    1992-01-01

    Los Alamos National Labs (LANL) is developing a new space sensor for examination of global warming effects: this sensor is called LARI (for Los Alamos Radiometric Instrument) and is a next generation follow-on to the ERBE instrument launched several years ago. Breault Research Organization, Inc. (BRO) is pleased to offer engineering consulting services for the development of this sensor. The goal of the consultation is to assist LANL engineers with the conceptual design of the LARI instrument. While the contract specifically stated that computer analyses would not be performed, we felt that some minimum level of computation effort would add more substance to the conclusions

  2. Embedded Sensor Systems for Health - A Step Towards Personalized Health.

    Science.gov (United States)

    Lindén, Maria; Björkman, Mats

    2018-01-01

    The demography is changing towards older people, and the challenge to provide an appropriate care is well known. Sensor systems, combined with IT solutions are recognized as one of the major tools to handle this situation. Embedded Sensor Systems for Health (ESS-H) is a research profile at Mälardalen University in Sweden, focusing on embedded sensor systems for health technology applications. The research addresses several important issues: to provide sensor systems for health monitoring at home, to provide sensor systems for health monitoring at work, to provide safe and secure infrastructure and software testing methods for physiological data management. The user perspective is important in order to solve real problems and to develop systems that are easy and intuitive to use. One of the overall aims is to enable health trend monitoring in home environments, thus being able to detect early deterioration of a patient. Sensor systems, signal processing algorithms, and decision support algorithms have been developed. Work on development of safe and secure infrastructure and software testing methods are important for an embedded sensor system aimed for health monitoring, both in home and in work applications. Patient data must be sent and received in a safe and secure manner, also fulfilling the integrity criteria.

  3. Intelligent Wireless Sensor Networks for System Health Monitoring

    Science.gov (United States)

    Alena, Rick

    2011-01-01

    Wireless sensor networks (WSN) based on the IEEE 802.15.4 Personal Area Network (PAN) standard are finding increasing use in the home automation and emerging smart energy markets. The network and application layers, based on the ZigBee 2007 Standard, provide a convenient framework for component-based software that supports customer solutions from multiple vendors. WSNs provide the inherent fault tolerance required for aerospace applications. The Discovery and Systems Health Group at NASA Ames Research Center has been developing WSN technology for use aboard aircraft and spacecraft for System Health Monitoring of structures and life support systems using funding from the NASA Engineering and Safety Center and Exploration Technology Development and Demonstration Program. This technology provides key advantages for low-power, low-cost ancillary sensing systems particularly across pressure interfaces and in areas where it is difficult to run wires. Intelligence for sensor networks could be defined as the capability of forming dynamic sensor networks, allowing high-level application software to identify and address any sensor that joined the network without the use of any centralized database defining the sensors characteristics. The IEEE 1451 Standard defines methods for the management of intelligent sensor systems and the IEEE 1451.4 section defines Transducer Electronic Datasheets (TEDS), which contain key information regarding the sensor characteristics such as name, description, serial number, calibration information and user information such as location within a vehicle. By locating the TEDS information on the wireless sensor itself and enabling access to this information base from the application software, the application can identify the sensor unambiguously and interpret and present the sensor data stream without reference to any other information. The application software is able to read the status of each sensor module, responding in real-time to changes of

  4. Cardiorespiratory system monitoring using a developed acoustic sensor.

    Science.gov (United States)

    Abbasi-Kesbi, Reza; Valipour, Atefeh; Imani, Khadije

    2018-02-01

    This Letter proposes a wireless acoustic sensor for monitoring heartbeat and respiration rate based on phonocardiogram (PCG). The developed sensor comprises a processor, a transceiver which operates at industrial, scientific and medical band and the frequency of 2.54 GHz as well as two capacitor microphones which one for recording the heartbeat and another one for respiration rate. To evaluate the precision of the presented sensor in estimating heartbeat and respiration rate, the sensor is tested on the different volunteers and the obtained results are compared with a gold standard as a reference. The results reveal that root-mean-square error are determined sensor estimate sounds of [Formula: see text] to [Formula: see text] obtained PCG signal with sensitivity and specificity 98.1% and 98.3% in turn that make 3% improvement than previous works. The results prove that the sensor can be appropriate candidate for recognising abnormal condition in the cardiorespiratory system.

  5. WISS - Wireless, Intelligent Sensor System, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Low power, robust communications protocols such as IEEE 802.15.4/Zigbee have made the concept of smart sensor networks attractive across many applications. These...

  6. Optimizing Systems of Threshold Detection Sensors

    National Research Council Canada - National Science Library

    Banschbach, David C

    2008-01-01

    .... Below the threshold all signals are ignored. We develop a mathematical model for setting individual sensor thresholds to obtain optimal probability of detecting a significant event, given a limit on the total number of false positives allowed...

  7. Nuclear Magnetic Resonance Trackbed Moisture Sensor System

    Science.gov (United States)

    2018-02-01

    In this initial phase, conducted from March 2015 through December 2016, Vista Clara and its subcontractor Zetica Rail successfully developed and tested a man-portable, non-invasive spot-check nuclear magnetic resonance (NMR) moisture sensor that dire...

  8. A Printed Organic Amplification System for Wearable Potentiometric Electrochemical Sensors.

    Science.gov (United States)

    Shiwaku, Rei; Matsui, Hiroyuki; Nagamine, Kuniaki; Uematsu, Mayu; Mano, Taisei; Maruyama, Yuki; Nomura, Ayako; Tsuchiya, Kazuhiko; Hayasaka, Kazuma; Takeda, Yasunori; Fukuda, Takashi; Kumaki, Daisuke; Tokito, Shizuo

    2018-03-02

    Electrochemical sensor systems with integrated amplifier circuits play an important role in measuring physiological signals via in situ human perspiration analysis. Signal processing circuitry based on organic thin-film transistors (OTFTs) have significant potential in realizing wearable sensor devices due to their superior mechanical flexibility and biocompatibility. Here, we demonstrate a novel potentiometric electrochemical sensing system comprised of a potassium ion (K + ) sensor and amplifier circuits employing OTFT-based pseudo-CMOS inverters, which have a highly controllable switching voltage and closed-loop gain. The ion concentration sensitivity of the fabricated K + sensor was 34 mV/dec, which was amplified to 160 mV/dec (by a factor of 4.6) with high linearity. The developed system is expected to help further the realization of ultra-thin and flexible wearable sensor devices for healthcare applications.

  9. Localisation system in wireless sensor networks using ns-2

    CSIR Research Space (South Africa)

    Abu-Mahfouz, Adnan M

    2012-04-01

    Full Text Available -1 /************************************************************************** ********** * * File: readme.asn * * Author: Adnan Abu-Mahfouz * * Date: March 2012 * * Description: Localisation system in wireless sensor networks using ns-2... *************************************************************************** *********/ /************************************************************************** *************************************************************************** *****/ 1. Introduction: ns-2 contains several flexible features that encourage researchers to use ns-2 to investigate the characteristics of wireless sensor networks (WSNs). However, to implement and evaluate localisation algorithms, the current ns- 2...

  10. Muscular condition monitoring system using fiber bragg grating sensors

    International Nuclear Information System (INIS)

    Kim, Heon Young; Lee, Jin Hyuk; Kim, Dae Hyun

    2014-01-01

    Fiber optic sensors (FOS) have advantages such as electromagnetic interference (EMI) immunity, corrosion resistance and multiplexing capability. For these reasons, they are widely used in various condition monitoring systems (CMS). This study investigated a muscular condition monitoring system using fiber optic sensors (FOS). Generally, sensors for monitoring the condition of the human body are based on electro-magnetic devices. However, such an electrical system has several weaknesses, including the potential for electro-magnetic interference and distortion. Fiber Bragg grating (FBG) sensors overcome these weaknesses, along with simplifying the devices and increasing user convenience. To measure the level of muscle contraction and relaxation, which indicates the muscle condition, a belt-shaped FBG sensor module that makes it possible to monitor the movement of muscles in the radial and circumferential directions was fabricated in this study. In addition, a uniaxial tensile test was carried out in order to evaluate the applicability of this FBG sensor module. Based on the experimental results, a relationship was observed between the tensile stress and Bragg wavelength of the FBG sensors, which revealed the possibility of fabricating a muscular condition monitoring system based on FBG sensors.

  11. Muscular condition monitoring system using fiber bragg grating sensors

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Heon Young; Lee, Jin Hyuk; Kim, Dae Hyun [Seoul National University of Technology, Seoul (Korea, Republic of)

    2014-10-15

    Fiber optic sensors (FOS) have advantages such as electromagnetic interference (EMI) immunity, corrosion resistance and multiplexing capability. For these reasons, they are widely used in various condition monitoring systems (CMS). This study investigated a muscular condition monitoring system using fiber optic sensors (FOS). Generally, sensors for monitoring the condition of the human body are based on electro-magnetic devices. However, such an electrical system has several weaknesses, including the potential for electro-magnetic interference and distortion. Fiber Bragg grating (FBG) sensors overcome these weaknesses, along with simplifying the devices and increasing user convenience. To measure the level of muscle contraction and relaxation, which indicates the muscle condition, a belt-shaped FBG sensor module that makes it possible to monitor the movement of muscles in the radial and circumferential directions was fabricated in this study. In addition, a uniaxial tensile test was carried out in order to evaluate the applicability of this FBG sensor module. Based on the experimental results, a relationship was observed between the tensile stress and Bragg wavelength of the FBG sensors, which revealed the possibility of fabricating a muscular condition monitoring system based on FBG sensors.

  12. Smart sensor systems for human health breath monitoring applications.

    Science.gov (United States)

    Hunter, G W; Xu, J C; Biaggi-Labiosa, A M; Laskowski, D; Dutta, P K; Mondal, S P; Ward, B J; Makel, D B; Liu, C C; Chang, C W; Dweik, R A

    2011-09-01

    Breath analysis techniques offer a potential revolution in health care diagnostics, especially if these techniques can be brought into standard use in the clinic and at home. The advent of microsensors combined with smart sensor system technology enables a new generation of sensor systems with significantly enhanced capabilities and minimal size, weight and power consumption. This paper discusses the microsensor/smart sensor system approach and provides a summary of efforts to migrate this technology into human health breath monitoring applications. First, the basic capability of this approach to measure exhaled breath associated with exercise physiology is demonstrated. Building from this foundation, the development of a system for a portable asthma home health care system is described. A solid-state nitric oxide (NO) sensor for asthma monitoring has been identified, and efforts are underway to miniaturize this NO sensor technology and integrate it into a smart sensor system. It is concluded that base platform microsensor technology combined with smart sensor systems can address the needs of a range of breath monitoring applications and enable new capabilities for healthcare.

  13. Ultrasonic Sensors in Urban Traffic Driving-Aid Systems

    Directory of Open Access Journals (Sweden)

    Teresa de Pedro

    2011-01-01

    Full Text Available Currently, vehicles are often equipped with active safety systems to reduce the risk of accidents, most of which occur in urban environments. The most prominent include Antilock Braking Systems (ABS, Traction Control and Stability Control. All these systems use different kinds of sensors to constantly monitor the conditions of the vehicle, and act in an emergency. In this paper the use of ultrasonic sensors in active safety systems for urban traffic is proposed, and the advantages and disadvantages when compared to other sensors are discussed. Adaptive Cruise Control (ACC for urban traffic based on ultrasounds is presented as an application example. The proposed system has been implemented in a fully-automated prototype vehicle and has been tested under real traffic conditions. The results confirm the good performance of ultrasonic sensors in these systems.

  14. Ultrasonic sensors in urban traffic driving-aid systems.

    Science.gov (United States)

    Alonso, Luciano; Milanés, Vicente; Torre-Ferrero, Carlos; Godoy, Jorge; Oria, Juan P; de Pedro, Teresa

    2011-01-01

    Currently, vehicles are often equipped with active safety systems to reduce the risk of accidents, most of which occur in urban environments. The most prominent include Antilock Braking Systems (ABS), Traction Control and Stability Control. All these systems use different kinds of sensors to constantly monitor the conditions of the vehicle, and act in an emergency. In this paper the use of ultrasonic sensors in active safety systems for urban traffic is proposed, and the advantages and disadvantages when compared to other sensors are discussed. Adaptive Cruise Control (ACC) for urban traffic based on ultrasounds is presented as an application example. The proposed system has been implemented in a fully-automated prototype vehicle and has been tested under real traffic conditions. The results confirm the good performance of ultrasonic sensors in these systems.

  15. Sensor Selection method for IoT systems – focusing on embedded system requirements

    Directory of Open Access Journals (Sweden)

    Hirayama Masayuki

    2016-01-01

    Full Text Available Recently, various types of sensors have been developed. Using these sensors, IoT systems have become hot topics in embedded system domain. However, sensor selections for embedded systems are not well discussed up to now. This paper focuses on embedded system’s features and architecture, and proposes a sensor selection method which is composed seven steps. In addition, we applied the proposed method to a simple example – a sensor selection for computer scored answer sheet reader unit. From this case study, an idea to use FTA in sensor selection is also discussed.

  16. A temperature and pressure controlled calibration system for pressure sensors

    Science.gov (United States)

    Chapman, John J.; Kahng, Seun K.

    1989-01-01

    A data acquisition and experiment control system capable of simulating temperatures from -184 to +220 C and pressures either absolute or differential from 0 to 344.74 kPa is developed to characterize silicon pressure sensor response to temperature and pressure. System software is described that includes sensor data acquisition, algorithms for numerically derived thermal offset and sensitivity correction, and operation of the environmental chamber and pressure standard. This system is shown to be capable of computer interfaced cryogenic testing to within 1 C and 34.47 Pa of single channel or multiplexed arrays of silicon pressure sensors.

  17. Sensor Buoy System for Monitoring Renewable Marine Energy Resources.

    Science.gov (United States)

    García, Emilio; Quiles, Eduardo; Correcher, Antonio; Morant, Francisco

    2018-03-22

    In this paper we present a multi-sensor floating system designed to monitor marine energy parameters, in order to sample wind, wave, and marine current energy resources. For this purpose, a set of dedicated sensors to measure the height and period of the waves, wind, and marine current intensity and direction have been selected and installed in the system. The floating device incorporates wind and marine current turbines for renewable energy self-consumption and to carry out complementary studies on the stability of such a system. The feasibility, safety, sensor communications, and buoy stability of the floating device have been successfully checked in real operating conditions.

  18. Application of wireless sensor network technology in logistics information system

    Science.gov (United States)

    Xu, Tao; Gong, Lina; Zhang, Wei; Li, Xuhong; Wang, Xia; Pan, Wenwen

    2017-04-01

    This paper introduces the basic concepts of active RFID (WSN-ARFID) based on wireless sensor networks and analyzes the shortcomings of the existing RFID-based logistics monitoring system. Integrated wireless sensor network technology and the scrambling point of RFID technology. A new real-time logistics detection system based on WSN and RFID, a model of logistics system based on WSN-ARFID is proposed, and the feasibility of this technology applied to logistics field is analyzed.

  19. Capillarity-based preparation system for optical colorimetric sensor arrays.

    Science.gov (United States)

    Luo, Xiao-Gang; Yi, Xin; Bu, Xiang-Nan; Hou, Chang-Jun; Huo, Dan-Qun; Yang, Mei; Fa, Huan-Bao; Lei, Jin-Can

    2017-03-01

    In recent years, optical colorimetric sensor arrays have demonstrated beneficial features, including rapid response, high selectivity, and high specificity; as a result, it has been extensively applied in food inspection and chemical studies, among other fields. There are instruments in the current market available for the preparation of an optical colorimetric sensor array, but it lacks the corresponding research of the preparation mechanism. Therefore, in connection with the main features of this kind of sensor array such as consistency, based on the preparation method of contact spotting, combined with a capillary fluid model, Washburn equation, Laplace equation, etc., this paper develops a diffusion model of an optical colorimetric sensor array during its preparation and sets up an optical colorimetric sensor array preparation system based on this diffusion model. Finally, this paper compares and evaluates the sensor arrays prepared by the system and prepared manually in three aspects such as the quality of array point, response of array, and response result, and the results show that the performance index of the sensor array prepared by a system under this diffusion model is better than that of the sensor array of manual spotting, which meets the needs of the experiment.

  20. CMOS-MEMS Chemiresistive and Chemicapacitive Chemical Sensor System

    Science.gov (United States)

    Lazarus, Nathan S.

    Integrating chemical sensors with testing electronics is a powerful technique with the potential to lower power and cost and allow for lower system limits of detection. This thesis explores the possibility of creating an integrated sensor system intended to be embedded within respirator cartridges to notify the user that hazardous chemicals will soon leak into the face mask. For a chemical sensor designer, this application is particularly challenging due to the need for a very sensitive and cheap sensor that will be exposed to widely varying environmental conditions during use. An octanethiol-coated gold nanoparticle chemiresistor to detect industrial solvents is developed, focusing on characterizing the environmental stability and limits of detection of the sensor. Since the chemiresistor was found to be highly sensitive to water vapor, a series of highly sensitive humidity sensor topologies were developed, with sensitivities several times previous integrated capacitive humidity sensors achieved. Circuit techniques were then explored to reduce the humidity sensor limits of detection, including the analysis of noise, charge injection, jitter and clock feedthrough in a charge-based capacitance measurement (CBCM) circuit and the design of a low noise Colpitts LC oscillator. The characterization of high resistance gold nanoclusters for capacitive chemical sensing was also performed. In the final section, a preconcentrator, a heater element intended to release a brief concentrated pulse of analate, was developed and tested for the purposes of lowering the system limit of detection.

  1. Patient Posture Monitoring System Based on Flexible Sensors

    Directory of Open Access Journals (Sweden)

    Youngsu Cha

    2017-03-01

    Full Text Available Monitoring patients using vision cameras can cause privacy intrusion problems. In this paper, we propose a patient position monitoring system based on a patient cloth with unobtrusive sensors. We use flexible sensors based on polyvinylidene fluoride, which is a flexible piezoelectric material. Theflexiblesensorsareinsertedintopartsclosetothekneeandhipoftheloosepatientcloth. We measure electrical signals from the sensors caused by the piezoelectric effect when the knee and hip in the cloth are bent. The measured sensor outputs are transferred to a computer via Bluetooth. We use a custom-made program to detect the position of the patient through a rule-based algorithm and the sensor outputs. The detectable postures are based on six human motions in and around a bed. The proposed system can detect the patient positions with a success rate over 88 percent for three patients.

  2. Optimal Sensor Networks Scheduling in Identification of Distributed Parameter Systems

    CERN Document Server

    Patan, Maciej

    2012-01-01

    Sensor networks have recently come into prominence because they hold the potential to revolutionize a wide spectrum of both civilian and military applications. An ingenious characteristic of sensor networks is the distributed nature of data acquisition. Therefore they seem to be ideally prepared for the task of monitoring processes with spatio-temporal dynamics which constitute one of most general and important classes of systems in modelling of the real-world phenomena. It is clear that careful deployment and activation of sensor nodes are critical for collecting the most valuable information from the observed environment. Optimal Sensor Network Scheduling in Identification of Distributed Parameter Systems discusses the characteristic features of the sensor scheduling problem, analyzes classical and recent approaches, and proposes a wide range of original solutions, especially dedicated for networks with mobile and scanning nodes. Both researchers and practitioners will find the case studies, the proposed al...

  3. Image acquisition system using on sensor compressed sampling technique

    Science.gov (United States)

    Gupta, Pravir Singh; Choi, Gwan Seong

    2018-01-01

    Advances in CMOS technology have made high-resolution image sensors possible. These image sensors pose significant challenges in terms of the amount of raw data generated, energy efficiency, and frame rate. This paper presents a design methodology for an imaging system and a simplified image sensor pixel design to be used in the system so that the compressed sensing (CS) technique can be implemented easily at the sensor level. This results in significant energy savings as it not only cuts the raw data rate but also reduces transistor count per pixel; decreases pixel size; increases fill factor; simplifies analog-to-digital converter, JPEG encoder, and JPEG decoder design; decreases wiring; and reduces the decoder size by half. Thus, CS has the potential to increase the resolution of image sensors for a given technology and die size while significantly decreasing the power consumption and design complexity. We show that it has potential to reduce power consumption by about 23% to 65%.

  4. Molecular Orientation in Two Component Vapor-Deposited Glasses: Effect of Substrate Temperature and Molecular Shape

    Science.gov (United States)

    Powell, Charles; Jiang, Jing; Walters, Diane; Ediger, Mark

    Vapor-deposited glasses are widely investigated for use in organic electronics including the emitting layers of OLED devices. These materials, while macroscopically homogenous, have anisotropic packing and molecular orientation. By controlling this orientation, outcoupling efficiency can be increased by aligning the transition dipole moment of the light-emitting molecules parallel to the substrate. Light-emitting molecules are typically dispersed in a host matrix, as such, it is imperative to understand molecular orientation in two-component systems. In this study we examine two-component vapor-deposited films and the orientations of the constituent molecules using spectroscopic ellipsometry, UV-vis and IR spectroscopy. The role of temperature, composition and molecular shape as it effects molecular orientation is examined for mixtures of DSA-Ph in Alq3 and in TPD. Deposition temperature relative to the glass transition temperature of the two-component mixture is the primary controlling factor for molecular orientation. In mixtures of DSA-Ph in Alq3, the linear DSA-Ph has a horizontal orientation at low temperatures and slight vertical orientation maximized at 0.96Tg,mixture, analogous to one-component films.

  5. Two-component fluid membranes near repulsive walls: Linearized hydrodynamics of equilibrium and nonequilibrium states.

    Science.gov (United States)

    Sankararaman, Sumithra; Menon, Gautam I; Sunil Kumar, P B

    2002-09-01

    We study the linearized hydrodynamics of a two-component fluid membrane near a repulsive wall, using a model that incorporates curvature-concentration coupling as well as hydrodynamic interactions. This model is a simplified version of a recently proposed one [J.-B. Manneville et al., Phys. Rev. E 64, 021908 (2001)] for nonequilibrium force centers embedded in fluid membranes, such as light-activated bacteriorhodopsin pumps incorporated in phospholipid egg phosphatidyl choline (EPC) bilayers. The pump-membrane system is modeled as an impermeable, two-component bilayer fluid membrane in the presence of an ambient solvent, in which one component, representing active pumps, is described in terms of force dipoles displaced with respect to the bilayer midpoint. We first discuss the case in which such pumps are rendered inactive, computing the mode structure in the bulk as well as the modification of hydrodynamic properties by the presence of a nearby wall. These results should apply, more generally, to equilibrium fluid membranes comprised of two components, in which the effects of curvature-concentration coupling are significant, above the threshold for phase separation. We then discuss the fluctuations and mode structure in the steady state of active two-component membranes near a repulsive wall. We find that proximity to the wall smoothens membrane height fluctuations in the stable regime, resulting in a logarithmic scaling of the roughness even for initially tensionless membranes. This explicitly nonequilibrium result is a consequence of the incorporation of curvature-concentration coupling in our hydrodynamic treatment. This result also indicates that earlier scaling arguments which obtained an increase in the roughness of active membranes near repulsive walls upon neglecting the role played by such couplings may need to be reevaluated.

  6. Sensor Failure Detection of FASSIP System using Principal Component Analysis

    Science.gov (United States)

    Sudarno; Juarsa, Mulya; Santosa, Kussigit; Deswandri; Sunaryo, Geni Rina

    2018-02-01

    In the nuclear reactor accident of Fukushima Daiichi in Japan, the damages of core and pressure vessel were caused by the failure of its active cooling system (diesel generator was inundated by tsunami). Thus researches on passive cooling system for Nuclear Power Plant are performed to improve the safety aspects of nuclear reactors. The FASSIP system (Passive System Simulation Facility) is an installation used to study the characteristics of passive cooling systems at nuclear power plants. The accuracy of sensor measurement of FASSIP system is essential, because as the basis for determining the characteristics of a passive cooling system. In this research, a sensor failure detection method for FASSIP system is developed, so the indication of sensor failures can be detected early. The method used is Principal Component Analysis (PCA) to reduce the dimension of the sensor, with the Squarred Prediction Error (SPE) and statistic Hotteling criteria for detecting sensor failure indication. The results shows that PCA method is capable to detect the occurrence of a failure at any sensor.

  7. Spin Valve Systems for Angle Sensor Applications

    OpenAIRE

    Johnson, Andrew

    2004-01-01

    A contact-less sensor with the ability to measure over a 360° range has been long sought after in the automotive industry. Such a sensor could be realized by utilizing the angle dependence of the Giant Magneto Resistance (GMR) Effect in a special type of magnetic multilayer called a spin valve arranged in a wheatstone bridge circuit [Spo96]. A spin valve consists of two ferromagnetic layers separated by nonmagnetic spacer layer where the magnetization of one of the ferromagnetic layers is pin...

  8. Active Multimodal Sensor System for Target Recognition and Tracking.

    Science.gov (United States)

    Qu, Yufu; Zhang, Guirong; Zou, Zhaofan; Liu, Ziyue; Mao, Jiansen

    2017-06-28

    High accuracy target recognition and tracking systems using a single sensor or a passive multisensor set are susceptible to external interferences and exhibit environmental dependencies. These difficulties stem mainly from limitations to the available imaging frequency bands, and a general lack of coherent diversity of the available target-related data. This paper proposes an active multimodal sensor system for target recognition and tracking, consisting of a visible, an infrared, and a hyperspectral sensor. The system makes full use of its multisensor information collection abilities; furthermore, it can actively control different sensors to collect additional data, according to the needs of the real-time target recognition and tracking processes. This level of integration between hardware collection control and data processing is experimentally shown to effectively improve the accuracy and robustness of the target recognition and tracking system.

  9. Cyber-physical system design with sensor networking technologies

    CERN Document Server

    Zeadally, Sherali

    2016-01-01

    This book describes how wireless sensor networking technologies can help in establishing and maintaining seamless communications between the physical and cyber systems to enable efficient, secure, reliable acquisition, management, and routing of data.

  10. Simple waves in a two-component Bose-Einstein condensate

    Science.gov (United States)

    Ivanov, S. K.; Kamchatnov, A. M.

    2018-04-01

    We study the dynamics of so-called simple waves in a two-component Bose-Einstein condensate. The evolution of the condensate is described by Gross-Pitaevskii equations which can be reduced for these simple wave solutions to a system of ordinary differential equations which coincide with those derived by Ovsyannikov for the two-layer fluid dynamics. We solve the Ovsyannikov system for two typical situations of large and small difference between interspecies and intraspecies nonlinear interaction constants. Our analytic results are confirmed by numerical simulations.

  11. A neuro-fuzzy inference system for sensor monitoring

    International Nuclear Information System (INIS)

    Na, Man Gyun

    2001-01-01

    A neuro-fuzzy inference system combined with the wavelet denoising, PCA (principal component analysis) and SPRT (sequential probability ratio test) methods has been developed to monitor the relevant sensor using the information of other sensors. The paramters of the neuro-fuzzy inference system which estimates the relevant sensor signal are optimized by a genetic algorithm and a least-squares algorithm. The wavelet denoising technique was applied to remove noise components in input signals into the neuro-fuzzy system. By reducing the dimension of an input space into the neuro-fuzzy system without losing a significant amount of information, the PCA was used to reduce the time necessary to train the neuro-fuzzy system, simplify the structure of the neuro-fuzzy inference system and also, make easy the selection of the input signals into the neuro-fuzzy system. By using the residual signals between the estimated signals and the measured signals, the SPRT is applied to detect whether the sensors are degraded or not. The proposed sensor-monitoring algorithm was verified through applications to the pressurizer water level, the pressurizer pressure, and the hot-leg temperature sensors in pressurized water reactors

  12. Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora.

    Science.gov (United States)

    Eriksson, A R; Andersson, R A; Pirhonen, M; Palva, E T

    1998-08-01

    Production of extracellular, plant cell wall degrading enzymes, the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, is coordinately controlled by a complex regulatory network. Insertion mutants in the exp (extracellular enzyme production) loci exhibit pleiotropic defects in virulence and the growth-phase-dependent transcriptional activation of genes encoding extracellular enzymes. Two new exp mutations, designated expA and expS, were characterized. Introduction of the corresponding wild-type alleles to the mutants complemented both the lack of virulence and the impaired production of plant cell wall degrading enzymes. The expA gene was shown to encode a 24-kDa polypeptide that is structurally and functionally related to the uvrY gene product of Escherichia coli and the GacA response regulator of Pseudomonas fluorescens. Functional similarity of expA and uvrY was demonstrated by genetic complementation. The expA gene is organized in an operon together with a uvrC-like gene, identical to the organization of uvrY and uvrC in E. coli. The unlinked expS gene encodes a putative sensor kinase that shows 92% identity to the recently described rpfA gene product from another E. carotovora subsp. carotovora strain. Our data suggest that ExpS and ExpA are members of two-component sensor kinase and response regulator families, respectively. These two proteins might interact in controlling virulence gene expression in E. carotovora subsp. carotovora.

  13. Wearable sensor system for human localization and motion capture

    OpenAIRE

    Zihajehzadeh, Shaghayegh

    2017-01-01

    Recent advances in MEMS wearable inertial/magnetic sensors and mobile computing have fostered a dramatic growth of interest for ambulatory human motion capture (MoCap). Compared to traditional optical MoCap systems such as the optical systems, inertial (i.e. accelerometer and gyroscope) and magnetic sensors do not require external fixtures such as cameras. Hence, they do not have in-the-lab measurement limitations and thus are ideal for ambulatory applications. However, due to the manufacturi...

  14. Nanopore sensors : From hybrid to abiotic systems

    NARCIS (Netherlands)

    Kocer, Armagan; Tauk, Lara; Dejardin, Philippe

    2012-01-01

    The use of nanopores of well controlled geometry for sensing molecules in solution is reviewed. Focus is concentrated especially on synthetic track-etch pores in polymer foils and on biological nanopores, i.e. ion channels. After a brief section about multipore sensors, specific attention is

  15. Semiautonomous Avionics-and-Sensors System for a UAV

    Science.gov (United States)

    Shams, Qamar

    2006-01-01

    Unmanned Aerial Vehicles (UAVs) autonomous or remotely controlled pilotless aircraft have been recently thrust into the spotlight for military applications, for homeland security, and as test beds for research. In addition to these functions, there are many space applications in which lightweight, inexpensive, small UAVS can be used e.g., to determine the chemical composition and other qualities of the atmospheres of remote planets. Moreover, on Earth, such UAVs can be used to obtain information about weather in various regions; in particular, they can be used to analyze wide-band acoustic signals to aid in determining the complex dynamics of movement of hurricanes. The Advanced Sensors and Electronics group at Langley Research Center has developed an inexpensive, small, integrated avionics-and-sensors system to be installed in a UAV that serves two purposes. The first purpose is to provide flight data to an AI (Artificial Intelligence) controller as part of an autonomous flight-control system. The second purpose is to store data from a subsystem of distributed MEMS (microelectromechanical systems) sensors. Examples of these MEMS sensors include humidity, temperature, and acoustic sensors, plus chemical sensors for detecting various vapors and other gases in the environment. The critical sensors used for flight control are a differential- pressure sensor that is part of an apparatus for determining airspeed, an absolute-pressure sensor for determining altitude, three orthogonal accelerometers for determining tilt and acceleration, and three orthogonal angular-rate detectors (gyroscopes). By using these eight sensors, it is possible to determine the orientation, height, speed, and rates of roll, pitch, and yaw of the UAV. This avionics-and-sensors system is shown in the figure. During the last few years, there has been rapid growth and advancement in the technological disciplines of MEMS, of onboard artificial-intelligence systems, and of smaller, faster, and

  16. Novel Hall sensors developed for magnetic field imaging systems

    International Nuclear Information System (INIS)

    Cambel, Vladimir; Karapetrov, Goran; Novosad, Valentyn; Bartolome, Elena; Gregusova, Dagmar; Fedor, Jan; Kudela, Robert; Soltys, Jan

    2007-01-01

    We report here on the fabrication and application of novel planar Hall sensors based on shallow InGaP/AlGaAs/GaAs heterostructure with a two-dimensional electron gas (2DEG) as an active layer. The sensors are developed for two kinds of experiments. In the first one, magnetic samples are placed directly on the Hall sensor. Room temperature experiments of permalloy objects evaporated onto the sensor are presented. In the second experiment, the sensor scans close over a multigranular superconducting sample prepared on a YBCO thin film. Large-area and high-resolution scanning experiments were performed at 4.2 K with the Hall probe scanning system in a liquid helium flow cryostat

  17. Optical detection system for MEMS-type pressure sensor

    International Nuclear Information System (INIS)

    Sareło, K; Górecka-Drzazga, A; Dziuban, J A

    2015-01-01

    In this paper a special optical detection system designed for a MEMS-type (micro-electro-mechanical system) silicon pressure sensor is presented. The main part of the optical system—a detection unit with a perforated membrane—is bonded to the silicon sensor, and placed in a measuring system. An external light source illuminates the membrane of the pressure sensor. Owing to the light reflected from the deflected membrane sensor, the optical pattern consisting of light points is visible, and pressure can be estimated. The optical detection unit (20   ×   20   ×   20.4 mm 3 ) is fabricated using microengineering techniques. Its dimensions are adjusted to the dimensions of the pressure sensor (5   ×   5 mm 2 silicon membrane). Preliminary tests of the optical detection unit integrated with the silicon pressure sensor are carried out. For the membrane sensor from 15 to 60 µm thick, a repeatable detection of the differential pressure in the range of 0 to 280 kPa is achieved. The presented optical microsystem is especially suitable for the pressure measurements in a high radiation environment. (paper)

  18. Research on MEMS sensor in hydraulic system flow detection

    Science.gov (United States)

    Zhang, Hongpeng; Zhang, Yindong; Liu, Dong; Ji, Yulong; Jiang, Jihai; Sun, Yuqing

    2011-05-01

    With the development of mechatronics technology and fault diagnosis theory, people regard flow information much more than before. Cheap, fast and accurate flow sensors are urgently needed by hydraulic industry. So MEMS sensor, which is small, low cost, well performed and easy to integrate, will surely play an important role in this field. Based on the new method of flow measurement which was put forward by our research group, this paper completed the measurement of flow rate in hydraulic system by setting up the mathematical model, using numerical simulation method and doing physical experiment. Based on viscous fluid flow equations we deduced differential pressure-velocity model of this new sensor and did optimization on parameters. Then, we designed and manufactured the throttle and studied the velocity and pressure field inside the sensor by FLUENT. Also in simulation we get the differential pressure-velocity curve .The model machine was simulated too to direct experiment. In the static experiments we calibrated the MEMS sensing element and built some sample sensors. Then in a hydraulic testing system we compared the sensor signal with a turbine meter. It presented good linearity and could meet general hydraulic system use. Based on the CFD curves, we analyzed the error reasons and made some suggestion to improve. In the dynamic test, we confirmed this sensor can realize high frequency flow detection by a 7 piston-pump.

  19. Transparent Fingerprint Sensor System for Large Flat Panel Display

    Directory of Open Access Journals (Sweden)

    Wonkuk Seo

    2018-01-01

    Full Text Available In this paper, we introduce a transparent fingerprint sensing system using a thin film transistor (TFT sensor panel, based on a self-capacitive sensing scheme. An armorphousindium gallium zinc oxide (a-IGZO TFT sensor array and associated custom Read-Out IC (ROIC are implemented for the system. The sensor panel has a 200 × 200 pixel array and each pixel size is as small as 50 μm × 50 μm. The ROIC uses only eight analog front-end (AFE amplifier stages along with a successive approximation analog-to-digital converter (SAR ADC. To get the fingerprint image data from the sensor array, the ROIC senses a capacitance, which is formed by a cover glass material between a human finger and an electrode of each pixel of the sensor array. Three methods are reviewed for estimating the self-capacitance. The measurement result demonstrates that the transparent fingerprint sensor system has an ability to differentiate a human finger’s ridges and valleys through the fingerprint sensor array.

  20. Transparent Fingerprint Sensor System for Large Flat Panel Display.

    Science.gov (United States)

    Seo, Wonkuk; Pi, Jae-Eun; Cho, Sung Haeung; Kang, Seung-Youl; Ahn, Seong-Deok; Hwang, Chi-Sun; Jeon, Ho-Sik; Kim, Jong-Uk; Lee, Myunghee

    2018-01-19

    In this paper, we introduce a transparent fingerprint sensing system using a thin film transistor (TFT) sensor panel, based on a self-capacitive sensing scheme. An armorphousindium gallium zinc oxide (a-IGZO) TFT sensor array and associated custom Read-Out IC (ROIC) are implemented for the system. The sensor panel has a 200 × 200 pixel array and each pixel size is as small as 50 μm × 50 μm. The ROIC uses only eight analog front-end (AFE) amplifier stages along with a successive approximation analog-to-digital converter (SAR ADC). To get the fingerprint image data from the sensor array, the ROIC senses a capacitance, which is formed by a cover glass material between a human finger and an electrode of each pixel of the sensor array. Three methods are reviewed for estimating the self-capacitance. The measurement result demonstrates that the transparent fingerprint sensor system has an ability to differentiate a human finger's ridges and valleys through the fingerprint sensor array.

  1. Structural integrated sensor and actuator systems for active flow control

    Science.gov (United States)

    Behr, Christian; Schwerter, Martin; Leester-Schädel, Monika; Wierach, Peter; Dietzel, Andreas; Sinapius, Michael

    2016-04-01

    An adaptive flow separation control system is designed and implemented as an essential part of a novel high-lift device for future aircraft. The system consists of MEMS pressure sensors to determine the flow conditions and adaptive lips to regulate the mass flow and the velocity of a wall near stream over the internally blown Coanda flap. By the oscillating lip the mass flow in the blowing slot changes dynamically, consequently the momentum exchange of the boundary layer over a high lift flap required mass flow can be reduced. These new compact and highly integrated systems provide a real-time monitoring and manipulation of the flow conditions. In this context the integration of pressure sensors into flow sensing airfoils of composite material is investigated. Mechanical and electrical properties of the integrated sensors are investigated under mechanical loads during tensile tests. The sensors contain a reference pressure chamber isolated to the ambient by a deformable membrane with integrated piezoresistors connected as a Wheatstone bridge, which outputs voltage signals depending on the ambient pressure. The composite material in which the sensors are embedded consists of 22 individual layers of unidirectional glass fiber reinforced plastic (GFRP) prepreg. The results of the experiments are used for adapting the design of the sensors and the layout of the laminate to ensure an optimized flux of force in highly loaded structures primarily for future aeronautical applications. It can be shown that the pressure sensor withstands the embedding process into fiber composites with full functional capability and predictable behavior under stress.

  2. Active Sensing System with In Situ Adjustable Sensor Morphology

    Science.gov (United States)

    Nurzaman, Surya G.; Culha, Utku; Brodbeck, Luzius; Wang, Liyu; Iida, Fumiya

    2013-01-01

    Background Despite the widespread use of sensors in engineering systems like robots and automation systems, the common paradigm is to have fixed sensor morphology tailored to fulfill a specific application. On the other hand, robotic systems are expected to operate in ever more uncertain environments. In order to cope with the challenge, it is worthy of note that biological systems show the importance of suitable sensor morphology and active sensing capability to handle different kinds of sensing tasks with particular requirements. Methodology This paper presents a robotics active sensing system which is able to adjust its sensor morphology in situ in order to sense different physical quantities with desirable sensing characteristics. The approach taken is to use thermoplastic adhesive material, i.e. Hot Melt Adhesive (HMA). It will be shown that the thermoplastic and thermoadhesive nature of HMA enables the system to repeatedly fabricate, attach and detach mechanical structures with a variety of shape and size to the robot end effector for sensing purposes. Via active sensing capability, the robotic system utilizes the structure to physically probe an unknown target object with suitable motion and transduce the arising physical stimuli into information usable by a camera as its only built-in sensor. Conclusions/Significance The efficacy of the proposed system is verified based on two results. Firstly, it is confirmed that suitable sensor morphology and active sensing capability enables the system to sense different physical quantities, i.e. softness and temperature, with desirable sensing characteristics. Secondly, given tasks of discriminating two visually indistinguishable objects with respect to softness and temperature, it is confirmed that the proposed robotic system is able to autonomously accomplish them. The way the results motivate new research directions which focus on in situ adjustment of sensor morphology will also be discussed. PMID:24416094

  3. Active sensing system with in situ adjustable sensor morphology.

    Science.gov (United States)

    Nurzaman, Surya G; Culha, Utku; Brodbeck, Luzius; Wang, Liyu; Iida, Fumiya

    2013-01-01

    Despite the widespread use of sensors in engineering systems like robots and automation systems, the common paradigm is to have fixed sensor morphology tailored to fulfill a specific application. On the other hand, robotic systems are expected to operate in ever more uncertain environments. In order to cope with the challenge, it is worthy of note that biological systems show the importance of suitable sensor morphology and active sensing capability to handle different kinds of sensing tasks with particular requirements. This paper presents a robotics active sensing system which is able to adjust its sensor morphology in situ in order to sense different physical quantities with desirable sensing characteristics. The approach taken is to use thermoplastic adhesive material, i.e. Hot Melt Adhesive (HMA). It will be shown that the thermoplastic and thermoadhesive nature of HMA enables the system to repeatedly fabricate, attach and detach mechanical structures with a variety of shape and size to the robot end effector for sensing purposes. Via active sensing capability, the robotic system utilizes the structure to physically probe an unknown target object with suitable motion and transduce the arising physical stimuli into information usable by a camera as its only built-in sensor. The efficacy of the proposed system is verified based on two results. Firstly, it is confirmed that suitable sensor morphology and active sensing capability enables the system to sense different physical quantities, i.e. softness and temperature, with desirable sensing characteristics. Secondly, given tasks of discriminating two visually indistinguishable objects with respect to softness and temperature, it is confirmed that the proposed robotic system is able to autonomously accomplish them. The way the results motivate new research directions which focus on in situ adjustment of sensor morphology will also be discussed.

  4. Method of estimating changes in vapor concentrations continuously generated from two-component organic solvents.

    Science.gov (United States)

    Hori, Hajime; Ishidao, Toru; Ishimatsu, Sumiyo

    2010-12-01

    We measured vapor concentrations continuously evaporated from two-component organic solvents in a reservoir and proposed a method to estimate and predict the evaporation rate or generated vapor concentrations. Two kinds of organic solvents were put into a small reservoir made of glass (3 cm in diameter and 3 cm high) that was installed in a cylindrical glass vessel (10 cm in diameter and 15 cm high). Air was introduced into the glass vessel at a flow rate of 150 ml/min, and the generated vapor concentrations were intermittently monitored for up to 5 hours with a gas chromatograph equipped with a flame ionization detector. The solvent systems tested in this study were the methanoltoluene system and the ethyl acetate-toluene system. The vapor concentrations of the more volatile component, that is, methanol in the methanol-toluene system and ethyl acetate in the ethyl acetate-toluene system, were high at first, and then decreased with time. On the other hand, the concentrations of the less volatile component were low at first, and then increased with time. A model for estimating multicomponent organic vapor concentrations was developed, based on a theory of vapor-liquid equilibria and a theory of the mass transfer rate, and estimated values were compared with experimental ones. The estimated vapor concentrations were in relatively good agreement with the experimental ones. The results suggest that changes in concentrations of two-component organic vapors continuously evaporating from a liquid reservoir can be estimated by the proposed model.

  5. State Estimation for Sensor Monitoring System with Uncertainty and Disturbance

    Directory of Open Access Journals (Sweden)

    Jianhong Sun

    2014-10-01

    Full Text Available This paper considers the state estimation problem for the sensor monitoring system which contains system uncertainty and nonlinear disturbance. In the sensor monitoring system, states of each inner sensor node usually contains system uncertainty, and external noise often works as nonlinear item. Besides, information transmission in the system is also time consuming. All mentioned above may arouse in unstable of the monitoring system. In this case, states of sensors could be wrongly sampled. Under this circumstance, a proper mathematical model is proposed and by the use of Lipschitz condition, the nonlinear item is transformed to linear one. In addition, we suppose that all sensor nodes are distributed arranged, no interface occurs with each other. By establishing proper Lyapunov– Krasovskii functional, sufficient conditions are acquired by solving linear matrix inequality to make the error augmented system stable, and the gains of observers are also derived. Finally, an illustrated example is given to show that system observed value tracks system states well, which fully demonstrate the effectiveness of our result.

  6. Wireless Zigbee strain gage sensor system for structural health monitoring

    Science.gov (United States)

    Ide, Hiroshi; Abdi, Frank; Miraj, Rashid; Dang, Chau; Takahashi, Tatsuya; Sauer, Bruce

    2009-05-01

    A compact cell phone size radio frequency (ZigBee) wireless strain measurement sensor system to measure the structural strain deformation was developed. The developed system provides an accurate strain measurement data stream to the Internet for further Diagnostic and Prognostic (DPS) correlation. Existing methods of structural measurement by strain sensors (gauges) do not completely satisfy problems posed by continuous structural health monitoring. The need for efficient health monitoring methods with real-time requirements to bidirectional data flow from sensors and to a commanding device is becoming critical for keeping our daily life safety. The use of full-field strain measurement techniques could reduce costly experimental programs through better understanding of material behavior. Wireless sensor-network technology is a monitoring method that is estimated to grow rapidly providing potential for cost savings over traditional wired sensors. The many of currently available wireless monitoring methods have: the proactive and constant data rate character of the data streams rather than traditional reactive, event-driven data delivery; mostly static node placement on structures with limited number of nodes. Alpha STAR Electronics' wireless sensor network system, ASWN, addresses some of these deficiencies, making the system easier to operate. The ASWN strain measurement system utilizes off-the-shelf sensors, namely strain gauges, with an analog-to-digital converter/amplifier and ZigBee radio chips to keep cost lower. Strain data is captured by the sensor, converted to digital form and delivered to the ZigBee radio chip, which in turn broadcasts the information using wireless protocols to a Personal Data Assistant (PDA) or Laptop/Desktop computers. From here, data is forwarded to remote computers for higher-level analysis and feedback using traditional cellular and satellite communication or the Ethernet infrastructure. This system offers a compact size, lower cost

  7. Two-component thermosensitive hydrogels : Phase separation affecting rheological behavior

    NARCIS (Netherlands)

    Abbadessa, Anna; Landín, Mariana; Oude Blenke, Erik; Hennink, Wim E.; Vermonden, Tina

    2017-01-01

    Extracellular matrices are mainly composed of a mixture of different biopolymers and therefore the use of two or more building blocks for the development of tissue-mimicking hydrogels is nowadays an attractive strategy in tissue-engineering. Multi-component hydrogel systems may undergo phase

  8. Convergence of PASTA kinase and two-component signaling in response to cell wall stress in Enterococcus faecalis.

    Science.gov (United States)

    Kellogg, Stephanie L; Kristich, Christopher J

    2018-04-09

    Two common signal transduction mechanisms used by bacteria to sense and respond to changing environments are two-component systems (TCSs) and eukaryotic-like Ser/Thr kinases and phosphatases (eSTK/Ps). Enterococcus faecalis is a Gram-positive bacterium and serious opportunistic pathogen that relies on both a TCS and an eSTK/P pathway for intrinsic resistance to cell wall-targeting antibiotics. The TCS consists of a histidine kinase (CroS) and response regulator (CroR) that become activated upon exposure of cells to cell wall-targeting antibiotics, leading to modulation of gene expression. The eSTK/P pathway consists of a transmembrane kinase (IreK) and its cognate phosphatase (IreP), which act antagonistically to mediate antibiotic resistance through an unknown mechanism. Because both CroS/R and IreK/P contribute to enterococcal resistance towards cell wall-targeting antibiotics, we hypothesized these signaling systems are intertwined. To test this hypothesis, we analyzed CroR phosphorylation and CroS/R-dependent gene expression to probe the influence of IreK and IreP on CroS/R signaling. In addition, we analyzed the phosphorylation state of CroS which revealed IreK-dependent phosphorylation of a Thr residue important for CroS function. Our results are consistent with a model in which IreK positively influences CroR-dependent gene expression through phosphorylation of CroS to promote antimicrobial resistance in E. faecalis Importance Two-component signaling systems (TCSs) and eukaryotic-like Ser/Thr kinases (eSTKs) are used by bacteria to sense and adapt to changing environments. Understanding how these pathways are regulated to promote bacterial survival is critical for a more complete understanding of bacterial stress responses and physiology. The opportunistic pathogen Enterococcus faecalis relies on both a TCS (CroS/R) and an eSTK (IreK) for intrinsic resistance to cell wall-targeting antibiotics. We probed the relationship between CroS/R and IreK, revealing

  9. Closed-loop System Identification with New Sensors

    DEFF Research Database (Denmark)

    Bendtsen, Jan Dimon; Trangbæk, K; Stoustrup, Jakob

    2008-01-01

    This paper deals with system identification of new system dynamics revealed by online introduction of new sensors in existing multi-variable linear control systems. The so-called "Hansen Scheme" utilises the dual Youla-Kucera parameterisation of all systems stabilised by a given linear controller...... to transform closed-loop system identification problems into open-loop-like problems. We show that this scheme can be formally extended to accomodate extra sensors in a nice way. The approach is illustrated on a simple simulation example....

  10. Calibrating a novel multi-sensor physical activity measurement system

    International Nuclear Information System (INIS)

    John, D; Sasaki, J E; Howe, C A; Freedson, P S; Liu, S; Gao, R X; Staudenmayer, J

    2011-01-01

    Advancing the field of physical activity (PA) monitoring requires the development of innovative multi-sensor measurement systems that are feasible in the free-living environment. The use of novel analytical techniques to combine and process these multiple sensor signals is equally important. This paper describes a novel multi-sensor 'integrated PA measurement system' (IMS), the lab-based methodology used to calibrate the IMS, techniques used to predict multiple variables from the sensor signals, and proposes design changes to improve the feasibility of deploying the IMS in the free-living environment. The IMS consists of hip and wrist acceleration sensors, two piezoelectric respiration sensors on the torso, and an ultraviolet radiation sensor to obtain contextual information (indoors versus outdoors) of PA. During lab-based calibration of the IMS, data were collected on participants performing a PA routine consisting of seven different ambulatory and free-living activities while wearing a portable metabolic unit (criterion measure) and the IMS. Data analyses on the first 50 adult participants are presented. These analyses were used to determine if the IMS can be used to predict the variables of interest. Finally, physical modifications for the IMS that could enhance the feasibility of free-living use are proposed and refinement of the prediction techniques is discussed

  11. A Magnetic Sensor System for Biological Detection

    KAUST Repository

    Li, Fuquan

    2015-05-01

    Magnetic biosensors detect biological targets through sensing the stray field of magnetic beads which label the targets. Commonly, magnetic biosensors employ the “sandwich” method to immobilize biological targets, i.e., the targets are sandwiched between a bio-functionalized sensor surface and bio-functionalized magnetic beads. This method has been used very successfully in different application, but its execution requires a rather elaborate procedure including several washing and incubation steps. This dissertation investigates a new magnetic biosensor concept, which enables a simple and effective detection of biological targets. The biosensor takes advantage of the size difference between bare magnetic beads and compounds of magnetic beads and biological targets. First, the detection of super-paramagnetic beads via magnetic tunnel junction (MTJ) sensors is implemented. Frequency modulation is used to enhance the signal-to-noise ratio, enabling the detection of a single magnetic bead. Second, the concept of the magnetic biosensor is investigated theoretically. The biosensor consists of an MTJ sensor, which detects the stray field of magnetic beads inside of a trap on top of the MTJ. A microwire between the trap and the MTJ is used to attract magnetic beads to the trapping well by applying a current to it. The MTJ sensor’s output depends on the number of beads inside the trap. If biological targets are in the sample solution, the beads will form bead compounds consisting of beads linked to the biological targets. Since bead compounds are larger than bare beads, the number of beads inside the trapping well will depend on the presence of biological targets. Hence, the output of the MTJ sensor will depend on the biological targets. The dependences of sensor signals on the sizes of the MTJ sensor, magnetic beads and biological targets are studied to find the optimum constellations for the detection of specific biological targets. The optimization is demonstrated

  12. Nonlinear light scattering in a two component medium: optical limiting application

    International Nuclear Information System (INIS)

    Joudrier, Valerie

    1998-01-01

    Scattering is a fundamental manifestation of the interaction between matter and radiation, resulting from inhomogeneities in the refractive index, which decrease transmission. This phenomenon is then especially attractive for sensor protection from laser light by optical limiting. One of the methods to induce scattering at high incident energy is to make use of the Kerr effect where the index of refraction is intensity dependent. Thus, the idea is to use a two component medium with a good index matching between the two components at low intensity, resulting in the medium transparency, and to modify it, at high intensity, due to the non linearity of one component making the medium highly scattering. Some of the experimental and theoretical investigations concerning a new material (here, a cell containing some liquid with small silica particles as inclusion in it) are presented in the visible domain (I=532 nm), for the nanosecond protection regime, beginning, with the chemical synthesis of the sample. The experimental results concerning the optical limiting process are presented, showing that nonlinear scattering is clearly the dominant mechanism in confrontation with other potential nonlinear effects. Several complementary experiments are then performed to complete the nonlinear scattering characterization, involving the measurement of the angular distribution of scattered energy and the integrating sphere measurement. Further information are also gained by studying the time response of the nonlinearities with a dual-beam (pulsed-pump, cw probe) technique. The previous experimental data is also analyzed with some simple theoretical models to evaluate the nonlinearity of the material from optical limiting, the angular scattering and the total scattering energy measurements. The good match between all the analytical results permits to delineate the physical mechanisms responsible for the nonlinear scattering effect and to direct the final conclusion. (author) [fr

  13. Trapping and Evolution Dynamics of Ultracold Two-Component Plasmas

    International Nuclear Information System (INIS)

    Choi, J.-H.; Knuffman, B.; Zhang, X. H.; Povilus, A. P.; Raithel, G.

    2008-01-01

    We demonstrate the trapping of a strongly magnetized, quasineutral ultracold plasma in a nested Penning trap with a background field of 2.9 T. Electrons remain trapped in this system for several milliseconds. Early in the evolution, the dynamics are driven by a breathing-mode oscillation in the ionic charge distribution, which modulates the electron trap depth. Over longer times scales, the electronic component undergoes cooling. Trap loss resulting from ExB drift is characterized

  14. Dynamics of a strongly driven two-component Bose-Einstein condensate

    International Nuclear Information System (INIS)

    Salmond, G.L.; Holmes, C.A.; Milburn, G.J.

    2002-01-01

    We consider a two-component Bose-Einstein condensate in two spatially localized modes of a double-well potential, with periodic modulation of the tunnel coupling between the two modes. We treat the driven quantum field using a two-mode expansion and define the quantum dynamics in terms of the Floquet Operator for the time periodic Hamiltonian of the system. It has been shown that the corresponding semiclassical mean-field dynamics can exhibit regions of regular and chaotic motion. We show here that the quantum dynamics can exhibit dynamical tunneling between regions of regular motion, centered on fixed points (resonances) of the semiclassical dynamics

  15. Stripes and honeycomb lattice of quantized vortices in rotating two-component Bose-Einstein condensates

    Science.gov (United States)

    Kasamatsu, Kenichi; Sakashita, Kouhei

    2018-05-01

    We study numerically the structure of a vortex lattice in rotating two-component Bose-Einstein condensates with equal atomic masses and equal intra- and intercomponent coupling strengths. The numerical simulations of the Gross-Pitaevskii equation show that the quantized vortices in this situation form lattice configuration accompanying vortex stripes, honeycomb lattices, and their complexes. This is a result of the degeneracy of the system for the SU(2) symmetric operation, which causes a continuous transformation between the above structures. In terms of the pseudospin representation, the complex lattice structures are identified as a hexagonal lattice of doubly winding half skyrmions.

  16. A smart sensor-based vision system: implementation and evaluation

    International Nuclear Information System (INIS)

    Elouardi, A; Bouaziz, S; Dupret, A; Lacassagne, L; Klein, J O; Reynaud, R

    2006-01-01

    One of the methods of solving the computational complexity of image-processing is to perform some low-level computations on the sensor focal plane. This paper presents a vision system based on a smart sensor. PARIS1 (Programmable Analog Retina-like Image Sensor1) is the first prototype used to evaluate the architecture of an on-chip vision system based on such a sensor coupled with a microcontroller. The smart sensor integrates a set of analog and digital computing units. This architecture paves the way for a more compact vision system and increases the performances reducing the data flow exchanges with a microprocessor in control. A system has been implemented as a proof-of-concept and has enabled us to evaluate the performance requirements for a possible integration of a microcontroller on the same chip. The used approach is compared with two architectures implementing CMOS active pixel sensors (APS) and interfaced to the same microcontroller. The comparison is related to image processing computation time, processing reliability, programmability, precision, bandwidth and subsequent stages of computations

  17. A smart sensor-based vision system: implementation and evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Elouardi, A; Bouaziz, S; Dupret, A; Lacassagne, L; Klein, J O; Reynaud, R [Institute of Fundamental Electronics, Bat. 220, Paris XI University, 91405 Orsay (France)

    2006-04-21

    One of the methods of solving the computational complexity of image-processing is to perform some low-level computations on the sensor focal plane. This paper presents a vision system based on a smart sensor. PARIS1 (Programmable Analog Retina-like Image Sensor1) is the first prototype used to evaluate the architecture of an on-chip vision system based on such a sensor coupled with a microcontroller. The smart sensor integrates a set of analog and digital computing units. This architecture paves the way for a more compact vision system and increases the performances reducing the data flow exchanges with a microprocessor in control. A system has been implemented as a proof-of-concept and has enabled us to evaluate the performance requirements for a possible integration of a microcontroller on the same chip. The used approach is compared with two architectures implementing CMOS active pixel sensors (APS) and interfaced to the same microcontroller. The comparison is related to image processing computation time, processing reliability, programmability, precision, bandwidth and subsequent stages of computations.

  18. Energy harvesting autonomous sensor systems design, analysis, and practical implementation

    CERN Document Server

    Tan, Yen Kheng

    2013-01-01

    This book is the considered the first to describe sensor-oriented energy harvesting issues. Its content is derived from the author's research on the development of a truly self-autonomous and sustainable energy harvesting wireless sensor network (EH-WSN). This network harvests energy from a variety of ambient energy sources and converts it into electrical energy to power batteries. The book discusses various types of energy harvesting (EH) systems and their respective main components.

  19. Sensor Deployment for Air Pollution Monitoring Using Public Transportation System

    OpenAIRE

    Yu, James J. Q.; Li, Victor O. K.; Lam, Albert Y. S.

    2015-01-01

    Air pollution monitoring is a very popular research topic and many monitoring systems have been developed. In this paper, we formulate the Bus Sensor Deployment Problem (BSDP) to select the bus routes on which sensors are deployed, and we use Chemical Reaction Optimization (CRO) to solve BSDP. CRO is a recently proposed metaheuristic designed to solve a wide range of optimization problems. Using the real world data, namely Hong Kong Island bus route data, we perform a series of simulations an...

  20. Two-component network model in voice identification technologies

    Directory of Open Access Journals (Sweden)

    Edita K. Kuular

    2018-03-01

    Full Text Available Among the most important parameters of biometric systems with voice modalities that determine their effectiveness, along with reliability and noise immunity, a speed of identification and verification of a person has been accentuated. This parameter is especially sensitive while processing large-scale voice databases in real time regime. Many research studies in this area are aimed at developing new and improving existing algorithms for presentation and processing voice records to ensure high performance of voice biometric systems. Here, it seems promising to apply a modern approach, which is based on complex network platform for solving complex massive problems with a large number of elements and taking into account their interrelationships. Thus, there are known some works which while solving problems of analysis and recognition of faces from photographs, transform images into complex networks for their subsequent processing by standard techniques. One of the first applications of complex networks to sound series (musical and speech analysis are description of frequency characteristics by constructing network models - converting the series into networks. On the network ontology platform a previously proposed technique of audio information representation aimed on its automatic analysis and speaker recognition has been developed. This implies converting information into the form of associative semantic (cognitive network structure with amplitude and frequency components both. Two speaker exemplars have been recorded and transformed into pertinent networks with consequent comparison of their topological metrics. The set of topological metrics for each of network models (amplitude and frequency one is a vector, and together  those combine a matrix, as a digital "network" voiceprint. The proposed network approach, with its sensitivity to personal conditions-physiological, psychological, emotional, might be useful not only for person identification

  1. Increased Efficiency of Face Recognition System using Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Rajani Muraleedharan

    2006-02-01

    Full Text Available This research was inspired by the need of a flexible and cost effective biometric security system. The flexibility of the wireless sensor network makes it a natural choice for data transmission. Swarm intelligence (SI is used to optimize routing in distributed time varying network. In this paper, SI maintains the required bit error rate (BER for varied channel conditions while consuming minimal energy. A specific biometric, the face recognition system, is discussed as an example. Simulation shows that the wireless sensor network is efficient in energy consumption while keeping the transmission accuracy, and the wireless face recognition system is competitive to the traditional wired face recognition system in classification accuracy.

  2. Systems and Methods for Automated Water Detection Using Visible Sensors

    Science.gov (United States)

    Rankin, Arturo L. (Inventor); Matthies, Larry H. (Inventor); Bellutta, Paolo (Inventor)

    2016-01-01

    Systems and methods are disclosed that include automated machine vision that can utilize images of scenes captured by a 3D imaging system configured to image light within the visible light spectrum to detect water. One embodiment includes autonomously detecting water bodies within a scene including capturing at least one 3D image of a scene using a sensor system configured to detect visible light and to measure distance from points within the scene to the sensor system, and detecting water within the scene using a processor configured to detect regions within each of the at least one 3D images that possess at least one characteristic indicative of the presence of water.

  3. An Environmental Monitoring System for Managing Spatiotemporal Sensor Data over Sensor Networks

    Directory of Open Access Journals (Sweden)

    Keun Ho Ryu

    2012-03-01

    Full Text Available In a wireless sensor network, sensors collect data about natural phenomena and transmit them to a server in real-time. Many studies have been conducted focusing on the processing of continuous queries in an approximate form. However, this approach is difficult to apply to environmental applications which require the correct data to be stored. In this paper, we propose a weather monitoring system for handling and storing the sensor data stream in real-time in order to support continuous spatial and/or temporal queries. In our system, we exploit two time-based insertion methods to store the sensor data stream and reduce the number of managed tuples, without losing any of the raw data which are useful for queries, by using the sensors’ temporal attributes. In addition, we offer a method for reducing the cost of the join operations used in processing spatiotemporal queries by filtering out a list of irrelevant sensors from query range before making a join operation. In the results of the performance evaluation, the number of tuples obtained from the data stream is reduced by about 30% in comparison to a naïve approach, thereby decreasing the query execution time.

  4. Multiobjective Design of Wearable Sensor Systems for Electrocardiogram Monitoring

    Directory of Open Access Journals (Sweden)

    F. J. Martinez-Tabares

    2016-01-01

    Full Text Available Wearable sensor systems will soon become part of the available medical tools for remote and long term physiological monitoring. However, the set of variables involved in the performance of these systems are usually antagonistic, and therefore the design of usable wearable systems in real clinical applications entails a number of challenges that have to be addressed first. This paper describes a method to optimise the design of these systems for the specific application of cardiac monitoring. The method proposed is based on the selection of a subset of 5 design variables, sensor contact, location, and rotation, signal correlation, and patient comfort, and 2 objective functions, functionality and wearability. These variables are optimised using linear and nonlinear models to maximise those objective functions simultaneously. The methodology described and the results achieved demonstrate that it is possible to find an optimal solution and therefore overcome most of the design barriers that prevent wearable sensor systems from being used in normal clinical practice.

  5. Electron beam diagnostic system using computed tomography and an annular sensor

    Science.gov (United States)

    Elmer, John W.; Teruya, Alan T.

    2014-07-29

    A system for analyzing an electron beam including a circular electron beam diagnostic sensor adapted to receive the electron beam, the circular electron beam diagnostic sensor having a central axis; an annular sensor structure operatively connected to the circular electron beam diagnostic sensor, wherein the sensor structure receives the electron beam; a system for sweeping the electron beam radially outward from the central axis of the circular electron beam diagnostic sensor to the annular sensor structure wherein the electron beam is intercepted by the annular sensor structure; and a device for measuring the electron beam that is intercepted by the annular sensor structure.

  6. Experimental Study of Nuclear Security System Components for Achieving the Intrusion Process via Sensor's Network System

    International Nuclear Information System (INIS)

    EL-Kafas, A.A.

    2011-01-01

    Cluster sensors are one of nuclear security system components which are used to detect any intrusion process of the nuclear sites. In this work, an experimental measuring test for sensor performance and procedures are presented. Sensor performance testing performed to determine whether a particular sensor will be acceptable in a proposed design. We have access to a sensors test field in which the sensor of interest is already properly installed and the parameters have been set to optimal levels by preliminary testing. The glass-breakage (G.B) and open door (O.D) sensors construction, operation and design for the investigated nuclear site are explained. Intrusion tests were carried out inside the field areas of the sensors to evaluate the sensor performance during the intrusion process. Experimental trials were performed for achieving the intrusion process via sensor network system. The performance and intrusion senses of cluster sensors inside the internal zones was recorded and evaluated. The obtained results explained that the tested and experimented G.B sensors have a probability of detection P (D) value 65% founded, and 80% P (D) of Open-door sensor

  7. A Mobile Sensor Network System for Monitoring of Unfriendly Environments.

    Science.gov (United States)

    Song, Guangming; Zhou, Yaoxin; Ding, Fei; Song, Aiguo

    2008-11-14

    Observing microclimate changes is one of the most popular applications of wireless sensor networks. However, some target environments are often too dangerous or inaccessible to humans or large robots and there are many challenges for deploying and maintaining wireless sensor networks in those unfriendly environments. This paper presents a mobile sensor network system for solving this problem. The system architecture, the mobile node design, the basic behaviors and advanced network capabilities have been investigated respectively. A wheel-based robotic node architecture is proposed here that can add controlled mobility to wireless sensor networks. A testbed including some prototype nodes has also been created for validating the basic functions of the proposed mobile sensor network system. Motion performance tests have been done to get the positioning errors and power consumption model of the mobile nodes. Results of the autonomous deployment experiment show that the mobile nodes can be distributed evenly into the previously unknown environments. It provides powerful support for network deployment and maintenance and can ensure that the sensor network will work properly in unfriendly environments.

  8. A multi-agent system architecture for sensor networks.

    Science.gov (United States)

    Fuentes-Fernández, Rubén; Guijarro, María; Pajares, Gonzalo

    2009-01-01

    The design of the control systems for sensor networks presents important challenges. Besides the traditional problems about how to process the sensor data to obtain the target information, engineers need to consider additional aspects such as the heterogeneity and high number of sensors, and the flexibility of these networks regarding topologies and the sensors in them. Although there are partial approaches for resolving these issues, their integration relies on ad hoc solutions requiring important development efforts. In order to provide an effective approach for this integration, this paper proposes an architecture based on the multi-agent system paradigm with a clear separation of concerns. The architecture considers sensors as devices used by an upper layer of manager agents. These agents are able to communicate and negotiate services to achieve the required functionality. Activities are organized according to roles related with the different aspects to integrate, mainly sensor management, data processing, communication and adaptation to changes in the available devices and their capabilities. This organization largely isolates and decouples the data management from the changing network, while encouraging reuse of solutions. The use of the architecture is facilitated by a specific modelling language developed through metamodelling. A case study concerning a generic distributed system for fire fighting illustrates the approach and the comparison with related work.

  9. A Multi-Agent System Architecture for Sensor Networks

    Directory of Open Access Journals (Sweden)

    María Guijarro

    2009-12-01

    Full Text Available The design of the control systems for sensor networks presents important challenges. Besides the traditional problems about how to process the sensor data to obtain the target information, engineers need to consider additional aspects such as the heterogeneity and high number of sensors, and the flexibility of these networks regarding topologies and the sensors in them. Although there are partial approaches for resolving these issues, their integration relies on ad hoc solutions requiring important development efforts. In order to provide an effective approach for this integration, this paper proposes an architecture based on the multi-agent system paradigm with a clear separation of concerns. The architecture considers sensors as devices used by an upper layer of manager agents. These agents are able to communicate and negotiate services to achieve the required functionality. Activities are organized according to roles related with the different aspects to integrate, mainly sensor management, data processing, communication and adaptation to changes in the available devices and their capabilities. This organization largely isolates and decouples the data management from the changing network, while encouraging reuse of solutions. The use of the architecture is facilitated by a specific modelling language developed through metamodelling. A case study concerning a generic distributed system for fire fighting illustrates the approach and the comparison with related work.

  10. Measurement system for special surface mapping using miniature displacement sensors

    Directory of Open Access Journals (Sweden)

    Zowade Martyna

    2018-01-01

    Full Text Available The aim of the work was to design a special system for measurements of elements with repetitive geometry or assemblies with repeating components, set in a linear patterns. The main focus was based on developing a computer program for signal analysis from variable number of miniature displacement sensors. It was set that the response for displacement of measuring tip from each sensor was a 0-5 V voltage signal with possibility of using different type of sensors. Requirements were determined based on projected measurement method. A special design of sensor was made for testing the computer program. If the characteristics of the sensor is known, it is possible to compute the type A evaluation of uncertainty. The results are presented in XY chart on computer screen. The program allows the user to choose any number of the sensors and determine the distance between them. Also, the possibility of calibration of sensors’ set was provided. The test were conducted on a prototype handle for sensors, made on a 3D printer.

  11. Sensor-actuator system for dynamic chloride ion determination.

    Science.gov (United States)

    de Graaf, Derk Balthazar; Abbas, Yawar; Gerrit Bomer, Johan; Olthuis, Wouter; van den Berg, Albert

    2015-08-12

    Chloride is a crucial anion for various analytical applications from biological to environmental applications. In order to measure the chloride ion concentration, a measurement system is needed which can detect this concentration for prolonged times reliably. Chronopotentiometry is a technique which does not need a long term stable reference electrode and is therefore very suitable for prolonged ion concentration measurements. As the used electrode might be fouled by reaction products, this work focuses on a chronopotentiometric approach with a separated sensing electrode (sensor) and actuating electrode (actuator). Both actuation and sensor electrode are made of Ag/AgCl. A constant current is applied to the actuator and will start the reaction between Ag and Cl-, while the resulting Cl- ion concentration change is observed through the sensor, which is placed close to the actuator. The time it takes to locally deplete the Cl- ions is called transition time. Experiments were performed to verify the feasibility of this approach. The performed experiments show that the sensor detects the local concentration changes resulting from the current applied to the actuator. A linear relation between the Cl- ion concentration and the square root of the transition time was observed, just as was predicted by theory. The calibration curves for different chips showed that both a larger sensor and a larger distance between sensor and actuator resulted in a larger time delay between the transition time detected at the actuator and the sensor. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Tablet PC Enabled Body Sensor System for Rural Telehealth Applications

    Directory of Open Access Journals (Sweden)

    Nitha V. Panicker

    2016-01-01

    Full Text Available Telehealth systems benefit from the rapid growth of mobile communication technology for measuring physiological signals. Development and validation of a tablet PC enabled noninvasive body sensor system for rural telehealth application are discussed in this paper. This system includes real time continuous collection of physiological parameters (blood pressure, pulse rate, and temperature and fall detection of a patient with the help of a body sensor unit and wireless transmission of the acquired information to a tablet PC handled by the medical staff in a Primary Health Center (PHC. Abnormal conditions are automatically identified and alert messages are given to the medical officer in real time. Clinical validation is performed in a real environment and found to be successful. Bland-Altman analysis is carried out to validate the wrist blood pressure sensor used. The system works well for all measurements.

  13. A Partially Distributed Intrusion Detection System for Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Eung Jun Cho

    2013-11-01

    Full Text Available The increasing use of wireless sensor networks, which normally comprise several very small sensor nodes, makes their security an increasingly important issue. They can be practically and efficiently secured using intrusion detection systems. Conventional security mechanisms are not usually applicable due to the sensor nodes having limitations of computational power, memory capacity, and battery power. Therefore, specific security systems should be designed to function under constraints of energy or memory. A partially distributed intrusion detection system with low memory and power demands is proposed here. It employs a Bloom filter, which allows reduced signature code size. Multiple Bloom filters can be combined to reduce the signature code for each Bloom filter array. The mechanism could then cope with potential denial of service attacks, unlike many previous detection systems with Bloom filters. The mechanism was evaluated and validated through analysis and simulation.

  14. Sensor failure detection in dynamical systems by Kalman filtering methodology

    International Nuclear Information System (INIS)

    Ciftcioglu, O.

    1991-03-01

    Design of a sensor failure detection system by Kalman filtering methodology is described. The method models the process systems in state-space form, the information on each state being provided by relevant sensors present in the process system. Since the measured states are usually subject to noise, the estimation of the states optimally is an essential requirement. To this end the detection system comprises Kalman estimation filters, the number of which is equal to the number of states concerned. The estimated state of a particular signal in each filter is compared with the corresponding measured signal and difference beyond a predetermined bound is identified as failure, the sensor being identified/isolated as faulty. (author). 19 refs.; 8 figs.; 1 tab

  15. Progress in triboluminescence-based smart optical sensor system

    International Nuclear Information System (INIS)

    Olawale, David O.; Dickens, Tarik; Sullivan, William G.; Okoli, Okenwa I.; Sobanjo, John O.; Wang, Ben

    2011-01-01

    Extensive research work has been done in recent times to apply the triboluminescence (TL) phenomenon for damage detection in engineering structures. Of particular note are the various attempts to apply it in the detection of impact damages in composites and aerospace structures. This is because TL-based sensor systems have a great potential for wireless, in-situ and distributed (WID) structural health monitoring when fully developed. This review article highlights development and the current state-of-the-art in the application of TL-based sensor systems. The underlying mechanisms believed to be responsible for triboluminescence, particularly in zinc sulfide manganese, a highly triboluminescent material, are discussed. The challenges militating against the full exploitation and field application of TL sensor systems are also identified. Finally, viable solutions and approaches to address these challenges are enumerated. - Highlights: → The underlying mechanisms believed to be responsible for triboluminescence. → State-of-the-art in the development and application of TL-based sensor systems. → The challenges militating against the full exploitation and field application of TL sensor systems are identified. → Viable solutions and approaches to address these challenges are enumerated.

  16. Sensors

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, H. [PBI-Dansensor A/S (Denmark); Toft Soerensen, O. [Risoe National Lab., Materials Research Dept. (Denmark)

    1999-10-01

    A new type of ceramic oxygen sensors based on semiconducting oxides was developed in this project. The advantage of these sensors compared to standard ZrO{sub 2} sensors is that they do not require a reference gas and that they can be produced in small sizes. The sensor design and the techniques developed for production of these sensors are judged suitable by the participating industry for a niche production of a new generation of oxygen sensors. Materials research on new oxygen ion conducting conductors both for applications in oxygen sensors and in fuel was also performed in this project and finally a new process was developed for fabrication of ceramic tubes by dip-coating. (EHS)

  17. Superfluid drag in the two-component Bose-Hubbard model

    Science.gov (United States)

    Sellin, Karl; Babaev, Egor

    2018-03-01

    In multicomponent superfluids and superconductors, co- and counterflows of components have, in general, different properties. A. F. Andreev and E. P. Bashkin [Sov. Phys. JETP 42, 164 (1975)] discussed, in the context of He3/He4 superfluid mixtures, that interparticle interactions produce a dissipationless drag. The drag can be understood as a superflow of one component induced by phase gradients of the other component. Importantly, the drag can be both positive (entrainment) and negative (counterflow). The effect is known to have crucial importance for many properties of diverse physical systems ranging from the dynamics of neutron stars and rotational responses of Bose mixtures of ultracold atoms to magnetic responses of multicomponent superconductors. Although substantial literature exists that includes the drag interaction phenomenologically, only a few regimes are covered by quantitative studies of the microscopic origin of the drag and its dependence on microscopic parameters. Here we study the microscopic origin and strength of the drag interaction in a quantum system of two-component bosons on a lattice with short-range interaction. By performing quantum Monte Carlo simulations of a two-component Bose-Hubbard model we obtain dependencies of the drag strength on the boson-boson interactions and properties of the optical lattice. Of particular interest are the strongly correlated regimes where the ratio of coflow and counterflow superfluid stiffnesses can diverge, corresponding to the case of saturated drag.

  18. Intelligence Control System for Landfills Based on Wireless Sensor Network

    Science.gov (United States)

    Zhang, Qian; Huang, Chuan; Gong, Jian

    2018-06-01

    This paper put forward an intelligence system for controlling the landfill gas in landfills to make the landfill gas (LFG) exhaust controllably and actively. The system, which is assigned by the wireless sensor network, were developed and supervised by remote applications in workshop instead of manual work. An automatic valve control depending on the sensor units embedded is installed in tube, the air pressure and concentration of LFG are detected to decide the level of the valve switch. The paper also proposed a modified algorithm to solve transmission problem, so that the system can keep a high efficiency and long service life.

  19. Simultaneous Sensor and Process Fault Diagnostics for Propellant Feed System

    Science.gov (United States)

    Cao, J.; Kwan, C.; Figueroa, F.; Xu, R.

    2006-01-01

    The main objective of this research is to extract fault features from sensor faults and process faults by using advanced fault detection and isolation (FDI) algorithms. A tank system that has some common characteristics to a NASA testbed at Stennis Space Center was used to verify our proposed algorithms. First, a generic tank system was modeled. Second, a mathematical model suitable for FDI has been derived for the tank system. Third, a new and general FDI procedure has been designed to distinguish process faults and sensor faults. Extensive simulations clearly demonstrated the advantages of the new design.

  20. Intelligence Control System for Landfills Based on Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Zhang Qian

    2018-01-01

    Full Text Available This paper put forward an intelligence system for controlling the landfill gas in landfills to make the landfill gas (LFG exhaust controllably and actively. The system, which is assigned by the wireless sensor network, were developed and supervised by remote applications in workshop instead of manual work. An automatic valve control depending on the sensor units embedded is installed in tube, the air pressure and concentration of LFG are detected to decide the level of the valve switch. The paper also proposed a modified algorithm to solve transmission problem, so that the system can keep a high efficiency and long service life.

  1. 30 CFR 75.1103-6 - Automatic fire sensors; actuation of fire suppression systems.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Automatic fire sensors; actuation of fire... Protection § 75.1103-6 Automatic fire sensors; actuation of fire suppression systems. Point-type heat sensors or automatic fire sensor and warning device systems may be used to actuate deluge-type water systems...

  2. Passive wireless sensor systems can recognize activites of daily living.

    Science.gov (United States)

    Urwyler, Prabitha; Stucki, Reto; Muri, Rene; Mosimann, Urs P; Nef, Tobias

    2015-08-01

    The ability to determine what activity of daily living a person performs is of interest in many application domains. It is possible to determine the physical and cognitive capabilities of the elderly by inferring what activities they perform in their houses. Our primary aim was to establish a proof of concept that a wireless sensor system can monitor and record physical activity and these data can be modeled to predict activities of daily living. The secondary aim was to determine the optimal placement of the sensor boxes for detecting activities in a room. A wireless sensor system was set up in a laboratory kitchen. The ten healthy participants were requested to make tea following a defined sequence of tasks. Data were collected from the eight wireless sensor boxes placed in specific places in the test kitchen and analyzed to detect the sequences of tasks performed by the participants. These sequence of tasks were trained and tested using the Markov Model. Data analysis focused on the reliability of the system and the integrity of the collected data. The sequence of tasks were successfully recognized for all subjects and the averaged data pattern of tasks sequences between the subjects had a high correlation. Analysis of the data collected indicates that sensors placed in different locations are capable of recognizing activities, with the movement detection sensor contributing the most to detection of tasks. The central top of the room with no obstruction of view was considered to be the best location to record data for activity detection. Wireless sensor systems show much promise as easily deployable to monitor and recognize activities of daily living.

  3. A Method of Data Aggregation for Wearable Sensor Systems

    Directory of Open Access Journals (Sweden)

    Bo Shen

    2016-06-01

    Full Text Available Data aggregation has been considered as an effective way to decrease the data to be transferred in sensor networks. Particularly for wearable sensor systems, smaller battery has less energy, which makes energy conservation in data transmission more important. Nevertheless, wearable sensor systems usually have features like frequently dynamic changes of topologies and data over a large range, of which current aggregating methods can’t adapt to the demand. In this paper, we study the system composed of many wearable devices with sensors, such as the network of a tactical unit, and introduce an energy consumption-balanced method of data aggregation, named LDA-RT. In the proposed method, we develop a query algorithm based on the idea of ‘happened-before’ to construct a dynamic and energy-balancing routing tree. We also present a distributed data aggregating and sorting algorithm to execute top-k query and decrease the data that must be transferred among wearable devices. Combining these algorithms, LDA-RT tries to balance the energy consumptions for prolonging the lifetime of wearable sensor systems. Results of evaluation indicate that LDA-RT performs well in constructing routing trees and energy balances. It also outperforms the filter-based top-k monitoring approach in energy consumption, load balance, and the network’s lifetime, especially for highly dynamic data sources.

  4. Intelligent Chemical Sensor Systems for In-space Safety Applications

    Science.gov (United States)

    Hunter, G. W.; Xu, J. C.; Neudeck, P. G.; Makel, D. B.; Ward, B.; Liu, C. C.

    2006-01-01

    Future in-space and lunar operations will require significantly improved monitoring and Integrated System Health Management (ISHM) throughout the mission. In particular, the monitoring of chemical species is an important component of an overall monitoring system for space vehicles and operations. For example, in leak monitoring of propulsion systems during launch, inspace, and on lunar surfaces, detection of low concentrations of hydrogen and other fuels is important to avoid explosive conditions that could harm personnel and damage the vehicle. Dependable vehicle operation also depends on the timely and accurate measurement of these leaks. Thus, the development of a sensor array to determine the concentration of fuels such as hydrogen, hydrocarbons, or hydrazine as well as oxygen is necessary. Work has been on-going to develop an integrated smart leak detection system based on miniaturized sensors to detect hydrogen, hydrocarbons, or hydrazine, and oxygen. The approach is to implement Microelectromechanical Systems (MEMS) based sensors incorporated with signal conditioning electronics, power, data storage, and telemetry enabling intelligent systems. The final sensor system will be self-contained with a surface area comparable to a postage stamp. This paper discusses the development of this "Lick and Stick" leak detection system and it s application to In-Space Transportation and other Exploration applications.

  5. Micro digital sun sensor: system in a package

    NARCIS (Netherlands)

    Boom, C.W. de; Leijtens, J.A.P.; Duivenbode, L.M.H. van; Heiden, N. van der

    2004-01-01

    A novel micro Digital Sun Sensor (μDSS) is under development in the frame of a micro systems technology (MST) development program (Microned) from the Dutch Ministry of Economic Affairs. Use of available micro system technologies in combination with the implementation of a dedicated solarcell for

  6. Airborne Electro-Optical Sensor Simulation System. Final Report.

    Science.gov (United States)

    Hayworth, Don

    The total system capability, including all the special purpose and general purpose hardware comprising the Airborne Electro-Optical Sensor Simulation (AEOSS) System, is described. The functional relationship between hardware portions is described together with interface to the software portion of the computer image generation. Supporting rationale…

  7. Characterization of a two-component thermoluminescent albedo dosemeter according to ISO 21909

    Energy Technology Data Exchange (ETDEWEB)

    Martins, M.M., E-mail: marcelo@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD/CNEN), Av. Salvador Allende s/n, CEP 22780-160, Rio de Janeiro, RJ (Brazil); Mauricio, C.L.P., E-mail: claudia@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD/CNEN), Av. Salvador Allende s/n, CEP 22780-160, Rio de Janeiro, RJ (Brazil); Pereira, W.W., E-mail: walsan@ird.gov.b [Instituto de Radioprotecao e Dosimetria (IRD/CNEN), Av. Salvador Allende s/n, CEP 22780-160, Rio de Janeiro, RJ (Brazil); Silva, A.X. da, E-mail: ademir@con.ufrj.b [Coordenacao dos Programas de Pos-Graduacao em Engenharia, COPPE/PEN Caixa Postal 68509, CEP 21941-972, Rio de Janeiro, RJ (Brazil)

    2011-05-15

    A two-component thermoluminescent albedo neutron monitoring system was developed at Instituto de Radioprotecao e Dosimetria, Brazil. As there is no Brazilian regulation for neutron individual monitoring service, the system was tested according to the ISO 21909 standard. This standard provides performance and test requirements for determining the acceptability of personal neutron dosemeters to be used for the measurement of personal dose equivalent, H{sub p}(10), in neutron fields with energies ranging from thermal to 20 MeV. Up to 40 dosemeters were used in order to accomplish satisfactorily the requirements of some tests. Despite operational difficulties, this albedo system passed all ISO 21909 performance requirements. The results and problems throughout this characterization are discussed in this paper.

  8. Wireless tamper detection sensor and sensing system

    Science.gov (United States)

    Woodard, Stanley E. (Inventor); Taylor, Bryant D. (Inventor)

    2011-01-01

    A wireless tamper detection sensor is defined by a perforated electrical conductor. The conductor is shaped to form a geometric pattern between first and second ends thereof such that the conductor defines an open-circuit that can store and transfer electrical and magnetic energy. The conductor resonates in the presence of a time-varying magnetic field to generate a harmonic response. The harmonic response changes when the conductor experiences a change in its geometric pattern due to severing of the conductor along at least a portion of the perforations. A magnetic field response recorder is used to wirelessly transmit the time-varying magnetic field and wirelessly detecting the conductor's harmonic response.

  9. Outlier Detection for Sensor Systems (ODSS): A MATLAB Macro for Evaluating Microphone Sensor Data Quality.

    Science.gov (United States)

    Vasta, Robert; Crandell, Ian; Millican, Anthony; House, Leanna; Smith, Eric

    2017-10-13

    Microphone sensor systems provide information that may be used for a variety of applications. Such systems generate large amounts of data. One concern is with microphone failure and unusual values that may be generated as part of the information collection process. This paper describes methods and a MATLAB graphical interface that provides rapid evaluation of microphone performance and identifies irregularities. The approach and interface are described. An application to a microphone array used in a wind tunnel is used to illustrate the methodology.

  10. Systems and Sensors for Debris-flow Monitoring and Warning

    Directory of Open Access Journals (Sweden)

    Lorenzo Marchi

    2008-04-01

    Full Text Available Debris flows are a type of mass movement that occurs in mountain torrents. They consist of a high concentration of solid material in water that flows as a wave with a steep front. Debris flows can be considered a phenomenon intermediate between landslides and water floods. They are amongst the most hazardous natural processes in mountainous regions and may occur under different climatic conditions. Their destructiveness is due to different factors: their capability of transporting and depositing huge amounts of solid materials, which may also reach large sizes (boulders of several cubic meters are commonly transported by debris flows, their steep fronts, which may reach several meters of height and also their high velocities. The implementation of both structural and nonstructural control measures is often required when debris flows endanger routes, urban areas and other infrastructures. Sensor networks for debris-flow monitoring and warning play an important role amongst non-structural measures intended to reduce debris-flow risk. In particular, debris flow warning systems can be subdivided into two main classes: advance warning and event warning systems. These two classes employ different types of sensors. Advance warning systems are based on monitoring causative hydrometeorological processes (typically rainfall and aim to issue a warning before a possible debris flow is triggered. Event warning systems are based on detecting debris flows when these processes are in progress. They have a much smaller lead time than advance warning ones but are also less prone to false alarms. Advance warning for debris flows employs sensors and techniques typical of meteorology and hydrology, including measuring rainfall by means of rain gauges and weather radar and monitoring water discharge in headwater streams. Event warning systems use different types of sensors, encompassing ultrasonic or radar gauges, ground vibration sensors, videocameras, avalanche

  11. A Novel Attitude Determination System Aided by Polarization Sensor

    Directory of Open Access Journals (Sweden)

    Wei Zhi

    2018-01-01

    Full Text Available This paper aims to develop a novel attitude determination system aided by polarization sensor. An improved heading angle function is derived using the perpendicular relationship between directions of E-vector of linearly polarized light and solar vector in the atmospheric polarization distribution model. The Extended Kalman filter (EKF with quaternion differential equation as a dynamic model is applied to fuse the data from sensors. The covariance functions of filter process and measurement noises are deduced in detail. The indoor and outdoor tests are conducted to verify the validity and feasibility of proposed attitude determination system. The test results showed that polarization sensor is not affected by magnetic field, thus the proposed system can work properly in environments containing the magnetic interference. The results also showed that proposed system has higher measurement accuracy than common attitude determination system and can provide precise parameters for Unmanned Aerial Vehicle (UAV flight control. The main contribution of this paper is implementation of the EKF for incorporating the self-developed polarization sensor into the conventional attitude determination system. The real-world experiment with the quad-rotor proved that proposed system can work in a magnetic interference environment and provide sufficient accuracy in attitude determination for autonomous navigation of vehicle.

  12. A Novel Attitude Determination System Aided by Polarization Sensor.

    Science.gov (United States)

    Zhi, Wei; Chu, Jinkui; Li, Jinshan; Wang, Yinlong

    2018-01-09

    This paper aims to develop a novel attitude determination system aided by polarization sensor. An improved heading angle function is derived using the perpendicular relationship between directions of E-vector of linearly polarized light and solar vector in the atmospheric polarization distribution model. The Extended Kalman filter (EKF) with quaternion differential equation as a dynamic model is applied to fuse the data from sensors. The covariance functions of filter process and measurement noises are deduced in detail. The indoor and outdoor tests are conducted to verify the validity and feasibility of proposed attitude determination system. The test results showed that polarization sensor is not affected by magnetic field, thus the proposed system can work properly in environments containing the magnetic interference. The results also showed that proposed system has higher measurement accuracy than common attitude determination system and can provide precise parameters for Unmanned Aerial Vehicle (UAV) flight control. The main contribution of this paper is implementation of the EKF for incorporating the self-developed polarization sensor into the conventional attitude determination system. The real-world experiment with the quad-rotor proved that proposed system can work in a magnetic interference environment and provide sufficient accuracy in attitude determination for autonomous navigation of vehicle.

  13. Multi-sensor measurement system for robotic drilling

    OpenAIRE

    Frommknecht, Andreas; Kühnle, Jens; Pidan, Sergej; Effenberger, Ira

    2015-01-01

    A multi-sensor measurement system for robotic drilling is presented. The system enables a robot to measure its 6D pose with respect to the work piece and to establish a reference coordinate system for drilling. The robot approaches the drill point and performs an orthogonal alignment with the work piece. Although the measurement systems are readily capable of achieving high position accuracy and low deviation to perpendicularity, experiments show that inaccuracies in the robot's 6D-pose and e...

  14. Operating systems and network protocols for wireless sensor networks.

    Science.gov (United States)

    Dutta, Prabal; Dunkels, Adam

    2012-01-13

    Sensor network protocols exist to satisfy the communication needs of diverse applications, including data collection, event detection, target tracking and control. Network protocols to enable these services are constrained by the extreme resource scarcity of sensor nodes-including energy, computing, communications and storage-which must be carefully managed and multiplexed by the operating system. These challenges have led to new protocols and operating systems that are efficient in their energy consumption, careful in their computational needs and miserly in their memory footprints, all while discovering neighbours, forming networks, delivering data and correcting failures.

  15. Sensor network infrastructure for a home care monitoring system.

    Science.gov (United States)

    Palumbo, Filippo; Ullberg, Jonas; Stimec, Ales; Furfari, Francesco; Karlsson, Lars; Coradeschi, Silvia

    2014-02-25

    This paper presents the sensor network infrastructure for a home care system that allows long-term monitoring of physiological data and everyday activities. The aim of the proposed system is to allow the elderly to live longer in their home without compromising safety and ensuring the detection of health problems. The system offers the possibility of a virtual visit via a teleoperated robot. During the visit, physiological data and activities occurring during a period of time can be discussed. These data are collected from physiological sensors (e.g., temperature, blood pressure, glucose) and environmental sensors (e.g., motion, bed/chair occupancy, electrical usage). The system can also give alarms if sudden problems occur, like a fall, and warnings based on more long-term trends, such as the deterioration of health being detected. It has been implemented and tested in a test environment and has been deployed in six real homes for a year-long evaluation. The key contribution of the paper is the presentation of an implemented system for ambient assisted living (AAL) tested in a real environment, combining the acquisition of sensor data, a flexible and adaptable middleware compliant with the OSGistandard and a context recognition application. The system has been developed in a European project called GiraffPlus.

  16. Automated wireless monitoring system for cable tension using smart sensors

    Science.gov (United States)

    Sim, Sung-Han; Li, Jian; Jo, Hongki; Park, Jongwoong; Cho, Soojin; Spencer, Billie F.; Yun, Chung-Bang

    2013-04-01

    Cables are critical load carrying members of cable-stayed bridges; monitoring tension forces of the cables provides valuable information for SHM of the cable-stayed bridges. Monitoring systems for the cable tension can be efficiently realized using wireless smart sensors in conjunction with vibration-based cable tension estimation approaches. This study develops an automated cable tension monitoring system using MEMSIC's Imote2 smart sensors. An embedded data processing strategy is implemented on the Imote2-based wireless sensor network to calculate cable tensions using a vibration-based method, significantly reducing the wireless data transmission and associated power consumption. The autonomous operation of the monitoring system is achieved by AutoMonitor, a high-level coordinator application provided by the Illinois SHM Project Services Toolsuite. The monitoring system also features power harvesting enabled by solar panels attached to each sensor node and AutoMonitor for charging control. The proposed wireless system has been deployed on the Jindo Bridge, a cable-stayed bridge located in South Korea. Tension forces are autonomously monitored for 12 cables in the east, land side of the bridge, proving the validity and potential of the presented tension monitoring system for real-world applications.

  17. Universal Properties of a Trapped Two-Component Fermi Gas at Unitarity

    International Nuclear Information System (INIS)

    Blume, D.; Stecher, J. von; Greene, Chris H.

    2007-01-01

    We treat the trapped two-component Fermi system, in which unlike fermions interact through a two-body short-range potential having no bound state but an infinite scattering length. By accurately solving the Schroedinger equation for up to N=6 fermions, we show that no many-body bound states exist other than those bound by the trapping potential, and we demonstrate unique universal properties of the system: Certain excitation frequencies are separated by 2(ℎ/2π)ω, the wave functions agree with analytical predictions and a virial theorem is fulfilled. Further calculations up to N=30 determine the excitation gap, an experimentally accessible universal quantity, and it agrees with recent predictions based on a density functional approach

  18. Modulational instability for a self-attractive two-component Bose–Einstein condensate

    International Nuclear Information System (INIS)

    Sheng-Chang, Li; Wen-Shan, Duan

    2009-01-01

    By means of the multiple-scale expansion method, the coupled nonlinear Schrödinger equations without an explicit external potential are obtained in two-dimensional geometry for a self-attractive Bose–Einstein condensate composed of different hyperfine states. The modulational instability of two-component condensate is investigated by using a simple technique. Based on the discussion about two typical cases, the explicit expression of the growth rate for a purely growing modulational instability and the optimum stable conditions are given and analysed analytically. The results show that the modulational instability of this two-dimensional system is quite different from that in a one-dimensional system. (general)

  19. A Neuron Model Based Ultralow Current Sensor System for Bioapplications

    Directory of Open Access Journals (Sweden)

    A. K. M. Arifuzzman

    2016-01-01

    Full Text Available An ultralow current sensor system based on the Izhikevich neuron model is presented in this paper. The Izhikevich neuron model has been used for its superior computational efficiency and greater biological plausibility over other well-known neuron spiking models. Of the many biological neuron spiking features, regular spiking, chattering, and neostriatal spiny projection spiking have been reproduced by adjusting the parameters associated with the model at hand. This paper also presents a modified interpretation of the regular spiking feature in which the firing pattern is similar to that of the regular spiking but with improved dynamic range offering. The sensor current ranges between 2 pA and 8 nA and exhibits linearity in the range of 0.9665 to 0.9989 for different spiking features. The efficacy of the sensor system in detecting low amount of current along with its high linearity attribute makes it very suitable for biomedical applications.

  20. Sensor Systems for Corrosion Monitoring in Concrete Structures

    Directory of Open Access Journals (Sweden)

    K.Kumar

    2006-05-01

    Full Text Available It is a need of permanently embedded corrosion monitoring devices to monitor the progress of corrosion problems on a new or existing reinforced concrete structures before embarking on repair or rehabilitation of the structures. Numerous devices are available for investigating corrosion problems, because no single technique exists which tells an engineer what he needs to know, namely how much damage there is on a structure now and how rapidly the damage will grow with time. In this investigation the studies on the sensors systems based on the measurements of half cell potential of rebars inside the concrete, resistivity of concrete, corrosion rate of rebars by eddy current measurements and sensing of chloride ions are reported. An integrated system consists of above sensors are fabricated and embedded into concrete. The response from each sensor was acquired and analyzed by NI hardware through LabVIEW software.

  1. Heme-based sensors in biological systems.

    Science.gov (United States)

    Rodgers, K R

    1999-04-01

    The past several years have been witness to a staggering rate of advancement in the understanding of how organisms respond to changes in the availability of diatomic molecules that are toxic and/or crucial to survival. Heme-based sensors presently constitute the majority of the proteins known to sense NO, O2 and CO and to initiate the chemistry required to adapt to changes in their availabilities. Knowledge of the three characterized members of this class, soluble guanylate cyclase, FixL and CooA, has grown substantially during the past year. The major advances have resulted from a broad range of approaches to elucidation of both function and mechanism. They include growth in the understanding of the interplay between the heme and protein in soluble guanylate cyclase, as well as alternate means for its stimulation. Insight into the O2-induced structural changes in FixL has been supplied by the single crystal structure of the heme domain of Bradyrhizobium japonicum. Finally, the ligation environment and ligand interchange that facilitates CO sensing by CooA has been established by spectroscopic and mutagenesis techniques.

  2. Hybrid Exploration Agent Platform and Sensor Web System

    Science.gov (United States)

    Stoffel, A. William; VanSteenberg, Michael E.

    2004-01-01

    A sensor web to collect the scientific data needed to further exploration is a major and efficient asset to any exploration effort. This is true not only for lunar and planetary environments, but also for interplanetary and liquid environments. Such a system would also have myriad direct commercial spin-off applications. The Hybrid Exploration Agent Platform and Sensor Web or HEAP-SW like the ANTS concept is a Sensor Web concept. The HEAP-SW is conceptually and practically a very different system. HEAP-SW is applicable to any environment and a huge range of exploration tasks. It is a very robust, low cost, high return, solution to a complex problem. All of the technology for initial development and implementation is currently available. The HEAP Sensor Web or HEAP-SW consists of three major parts, The Hybrid Exploration Agent Platforms or HEAP, the Sensor Web or SW and the immobile Data collection and Uplink units or DU. The HEAP-SW as a whole will refer to any group of mobile agents or robots where each robot is a mobile data collection unit that spends most of its time acting in concert with all other robots, DUs in the web, and the HEAP-SWs overall Command and Control (CC) system. Each DU and robot is, however, capable of acting independently. The three parts of the HEAP-SW system are discussed in this paper. The Goals of the HEAP-SW system are: 1) To maximize the amount of exploration enhancing science data collected; 2) To minimize data loss due to system malfunctions; 3) To minimize or, possibly, eliminate the risk of total system failure; 4) To minimize the size, weight, and power requirements of each HEAP robot; 5) To minimize HEAP-SW system costs. The rest of this paper discusses how these goals are attained.

  3. Viscous Growth in Spinodal Decomposition of the Two-component Lennard-Jones Model in Two Dimensions

    DEFF Research Database (Denmark)

    Laradji, M.; Toxvaerd, S.; Mouritsen, Ole G.

    1997-01-01

    The dynamics of phase separation of a two-component Lennard-Jones model in three dimensions is investigated by means of large scale molecular dynamics simulation. A systematic study over a wide range of quench temperatures within the coexistence region shows that the binary system reaches...

  4. Consistent Steering System using SCTP for Bluetooth Scatternet Sensor Network

    Science.gov (United States)

    Dhaya, R.; Sadasivam, V.; Kanthavel, R.

    2012-12-01

    Wireless communication is the best way to convey information from source to destination with flexibility and mobility and Bluetooth is the wireless technology suitable for short distance. On the other hand a wireless sensor network (WSN) consists of spatially distributed autonomous sensors to cooperatively monitor physical or environmental conditions, such as temperature, sound, vibration, pressure, motion or pollutants. Using Bluetooth piconet wireless technique in sensor nodes creates limitation in network depth and placement. The introduction of Scatternet solves the network restrictions with lack of reliability in data transmission. When the depth of the network increases, it results in more difficulties in routing. No authors so far focused on the reliability factors of Scatternet sensor network's routing. This paper illustrates the proposed system architecture and routing mechanism to increase the reliability. The another objective is to use reliable transport protocol that uses the multi-homing concept and supports multiple streams to prevent head-of-line blocking. The results show that the Scatternet sensor network has lower packet loss even in the congestive environment than the existing system suitable for all surveillance applications.

  5. Wireless energizing system for an automated implantable sensor

    Energy Technology Data Exchange (ETDEWEB)

    Swain, Biswaranjan; Nayak, Praveen P.; Kar, Durga P.; Bhuyan, Satyanarayan; Mishra, Laxmi P. [Department of Electronics and Instrumentation Engineering, Siksha ‘O’ Anusandhan University, Bhubaneswar 751030 (India)

    2016-07-15

    The wireless drive of an automated implantable electronic sensor has been explored for health monitoring applications. The proposed system comprises of an automated biomedical sensing system which is energized through resonant inductive coupling. The implantable sensor unit is able to monitor the body temperature parameter and sends back the corresponding telemetry data wirelessly to the data recoding unit. It has been observed that the wireless power delivery system is capable of energizing the automated biomedical implantable electronic sensor placed over a distance of 3 cm from the power transmitter with an energy transfer efficiency of 26% at the operating resonant frequency of 562 kHz. This proposed method ensures real-time monitoring of different human body temperatures around the clock. The monitored temperature data have been compared with a calibrated temperature measurement system to ascertain the accuracy of the proposed system. The investigated technique can also be useful for monitoring other body parameters such as blood pressure, bladder pressure, and physiological signals of the patient in vivo using various implantable sensors.

  6. Wireless energizing system for an automated implantable sensor

    International Nuclear Information System (INIS)

    Swain, Biswaranjan; Nayak, Praveen P.; Kar, Durga P.; Bhuyan, Satyanarayan; Mishra, Laxmi P.

    2016-01-01

    The wireless drive of an automated implantable electronic sensor has been explored for health monitoring applications. The proposed system comprises of an automated biomedical sensing system which is energized through resonant inductive coupling. The implantable sensor unit is able to monitor the body temperature parameter and sends back the corresponding telemetry data wirelessly to the data recoding unit. It has been observed that the wireless power delivery system is capable of energizing the automated biomedical implantable electronic sensor placed over a distance of 3 cm from the power transmitter with an energy transfer efficiency of 26% at the operating resonant frequency of 562 kHz. This proposed method ensures real-time monitoring of different human body temperatures around the clock. The monitored temperature data have been compared with a calibrated temperature measurement system to ascertain the accuracy of the proposed system. The investigated technique can also be useful for monitoring other body parameters such as blood pressure, bladder pressure, and physiological signals of the patient in vivo using various implantable sensors.

  7. Wireless energizing system for an automated implantable sensor.

    Science.gov (United States)

    Swain, Biswaranjan; Nayak, Praveen P; Kar, Durga P; Bhuyan, Satyanarayan; Mishra, Laxmi P

    2016-07-01

    The wireless drive of an automated implantable electronic sensor has been explored for health monitoring applications. The proposed system comprises of an automated biomedical sensing system which is energized through resonant inductive coupling. The implantable sensor unit is able to monitor the body temperature parameter and sends back the corresponding telemetry data wirelessly to the data recoding unit. It has been observed that the wireless power delivery system is capable of energizing the automated biomedical implantable electronic sensor placed over a distance of 3 cm from the power transmitter with an energy transfer efficiency of 26% at the operating resonant frequency of 562 kHz. This proposed method ensures real-time monitoring of different human body temperatures around the clock. The monitored temperature data have been compared with a calibrated temperature measurement system to ascertain the accuracy of the proposed system. The investigated technique can also be useful for monitoring other body parameters such as blood pressure, bladder pressure, and physiological signals of the patient in vivo using various implantable sensors.

  8. Atmospheric turbulence and sensor system effects on biometric algorithm performance

    Science.gov (United States)

    Espinola, Richard L.; Leonard, Kevin R.; Byrd, Kenneth A.; Potvin, Guy

    2015-05-01

    Biometric technologies composed of electro-optical/infrared (EO/IR) sensor systems and advanced matching algorithms are being used in various force protection/security and tactical surveillance applications. To date, most of these sensor systems have been widely used in controlled conditions with varying success (e.g., short range, uniform illumination, cooperative subjects). However the limiting conditions of such systems have yet to be fully studied for long range applications and degraded imaging environments. Biometric technologies used for long range applications will invariably suffer from the effects of atmospheric turbulence degradation. Atmospheric turbulence causes blur, distortion and intensity fluctuations that can severely degrade image quality of electro-optic and thermal imaging systems and, for the case of biometrics technology, translate to poor matching algorithm performance. In this paper, we evaluate the effects of atmospheric turbulence and sensor resolution on biometric matching algorithm performance. We use a subset of the Facial Recognition Technology (FERET) database and a commercial algorithm to analyze facial recognition performance on turbulence degraded facial images. The goal of this work is to understand the feasibility of long-range facial recognition in degraded imaging conditions, and the utility of camera parameter trade studies to enable the design of the next generation biometrics sensor systems.

  9. MzrA: a novel modulator of the EnvZ/OmpR two-component regulon

    Science.gov (United States)

    Gerken, Henri; Charlson, Emily S; Cicirelli, Elisha M; Kenney, Linda J; Misra, Rajeev

    2009-01-01

    Analysis of suppressors that alleviate the acute envelope stress phenotype of a ΔbamBΔdegP strain of Escherichia coli identified a novel protein MzrA and pleiotropic envZ mutations. Genetic evidence shows that overexpression of MzrA – formerly known as YqjB and EcfM – modulates the activity of EnvZ/OmpR similarly to pleiotropic EnvZ mutants and alter porin expression. However, porin expression in strains devoid of MzrA or overexpressing it is still sensitive to medium osmolarity, pH and procaine, all of which modulate EnvZ/OmpR activities. Thus, MzrA appears to alter the output of the EnvZ/OmpR system but not its ability to receive and respond to various environmental signals. Localization and topology experiments indicate that MzrA is a type II membrane protein, with its N-terminus exposed in the cytoplasm and C-terminus in the periplasm. Bacterial two-hybrid experiments determined that MzrA specifically interacts with EnvZ but not with OmpR or the related membrane sensor kinase, CpxA. This and additional genetic and biochemical evidence suggest that the interaction of MzrA with EnvZ would either enhance EnvZ's kinase activity or reduce its phosphatase activity, thus elevating the steady state levels of OmpR∼P. Furthermore, our data show that MzrA links the two-component envelope stress response regulators, CpxA/CpxR and EnvZ/OmpR. PMID:19432797

  10. Pollution Monitoring System Using Gas Sensor based on Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    M. Udin Harun Al Rasyid

    2016-01-01

    Full Text Available Carbon monoxide (CO and carbon dioxide (CO2 gases are classified as colorless and odorless gas so we need special tools to monitor their concentration in the air. Concentration of air pollution of CO and CO2 that are high in the air will give serious effects for health status. CO is a poisonous gas that damages the circulation of oxygen in the blood when inhaled, while CO2 is one of the gases that causes global warming. In this paper, we developed an integrated pollution monitoring (IPOM system to monitor the concentration of air pollution. This research implemented three sensor nodes (end-device which each node contains CO and CO2 sensors on the gas sensors board to perform sensing from the environment. Furthermore, the data taken from the environment by the sensor will be sent to the meshlium gateway using IEEE 802.15.4 Zigbee communications and processed by the gateway in order to be sent to the computer server. The data is stored in meshlium gateway using MySQL database as a backup, and it will be synchronized to the MySQL database in the computer server. We provide services for public to access the information in database server through a desktop and website application.

  11. Parking Sensing and Information System: Sensors, Deployment, and Evaluation

    OpenAIRE

    Chen, Xiao; Zhen; Qian; Rajagopal, Ram; Stiers, Todd; Flores, Christopher; Kavaler, Robert; Williams III, Floyd

    2017-01-01

    This paper describes a smart parking sensing and information system that disseminates the parking availability information for public users in a cost-effective and efficient manner. The hardware framework of the system is built on advanced wireless sensor networks and cloud service over the Internet, and the system is highly scalable. The parking information provided to the users is set in the form of occupancy rates and expected cruising time. Both are obtained from our analytical algorithm ...

  12. Intelligent Braking System using the IR Sensor

    OpenAIRE

    Gajanan Koli

    2017-01-01

    Most of the accidents in four wheeled vehicles occur because of failure of braking systems. Manual method of applying brakes is always dangerous as it leads to accidents. Unconsciousness of driver, failure in the linkages of braking systems, road conditions, uncontrollable speed of the vehicle and manual operation of braking systems are the reasons of accidents. It is necessary to control brakes automatically through electronics devices to minimize the accident problems. In this research pape...

  13. Bedside arterial blood gas monitoring system using fluorescent optical sensors

    Science.gov (United States)

    Bartnik, Daniel J.; Rymut, Russell A.

    1995-05-01

    We describe a bedside arterial blood gas (ABG) monitoring system which uses fluorescent optical sensors in the measurement of blood pH, PCO2 and PO2. The Point-of-Care Arterial Blood Gas Monitoring System consists of the SensiCathTM optical sensor unit manufactured by Optical Sensors Incorporated and the TramTM Critical Care Monitoring System with ABG Module manufactured by Marquette Electronics Incorporated. Current blood gas measurement techniques require a blood sample to be removed from the patient and transported to an electrochemical analyzer for analysis. The ABG system does not require removal of blood from the patient or transport of the sample. The sensor is added to the patient's existing arterial line. ABG measurements are made by drawing a small blood sample from the arterial line in sufficient quantity to ensure an undiluted sample at the sensor. Measurements of pH, PCO2 and PO2 are made within 60 seconds. The blood is then returned to the patient, the line flushed and results appear on the bedside monitor. The ABG system offers several advantages over traditional electrochemical analyzers. Since the arterial line remains closed during the blood sampling procedure the patient's risk of infection is reduced and the caregiver's exposure to blood is eliminated. The single-use, disposable sensor can be measure 100 blood samples over 72 hours after a single two-point calibration. Quality Assurance checks are also available and provide the caregiver the ability to assess system performance even after the sensor is patient attached. The ABG module integrates with an existing bedside monitoring system. This allows ABG results to appear on the same display as ECG, respiration, blood pressure, cardiac output, SpO2, and other clinical information. The small module takes up little space in the crowded intensive care unit. Performance studies compare the ABG system with an electrochemical blood gas analyzer. Study results demonstrated accurate and precise blood

  14. Packaged Capacitive Pressure Sensor System for Aircraft Engine Health Monitoring

    Science.gov (United States)

    Scardelletti, Maximilian C.; Zorman, Christian A.

    2016-01-01

    This paper describes the development of a packaged silicon carbide (SiC) based MEMS pressure sensor system designed specifically for a conventional turbofan engine. The electronic circuit is based on a Clapp-type oscillator that incorporates a 6H-SiC MESFET, a SiCN MEMS capacitive pressure sensor, titanate MIM capacitors, wirewound inductors, and thick film resistors. The pressure sensor serves as the capacitor in the LC tank circuit, thereby linking pressure to the resonant frequency of the oscillator. The oscillator and DC bias circuitry were fabricated on an alumina substrate and secured inside a metal housing. The packaged sensing system reliably operates at 0 to 350 psi and 25 to 540C. The system has a pressure sensitivity of 6.8 x 10E-2 MHzpsi. The packaged system shows negligible difference in frequency response between 25 and 400C. The fully packaged sensor passed standard benchtop acceptance tests and was evaluated on a flight-worthy engine.

  15. Mechatronic sensor system for robots and automated machines

    CSIR Research Space (South Africa)

    Shaik, AA

    2007-01-01

    Full Text Available machine makes a calculated estimate of where the tool-head should be. This is often achieved by monitoring sensors on axes that track linear translation and rotations of shafts or gears. For low precision applications this system is appropriate. However...

  16. New portable sensor system for rotation seismic motion measurements

    Czech Academy of Sciences Publication Activity Database

    Brokešová, J.; Málek, Jiří

    2010-01-01

    Roč. 81, č. 8 (2010), 084501 ISSN 0034-6748 R&D Projects: GA ČR GAP210/10/0925 Institutional research plan: CEZ:AV0Z30460519 Keywords : rotation al seismology * sensor system Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 1.598, year: 2010

  17. On-line methanol sensor system development for recombinant ...

    African Journals Online (AJOL)

    PANCHIGA

    2016-10-19

    Oct 19, 2016 ... Calibration of the methanol sensor system was done in a medium environment with ... by taking protein induction at a low temperature and a pH where protease ... molecular weight of 66.5 kDa, HSA comprises about one-.

  18. A new VFA sensor technique for anaerobic reactor systems

    DEFF Research Database (Denmark)

    Pind, Peter Frode; Angelidaki, Irini; Ahring, Birgitte Kiær

    2003-01-01

    , propionate, iso-/n-butyrate and iso-/n-valerate ranging from 0.1 to 50 mM (6-3000 mg). The measuring range could readily be expanded to more components and both lower and higher concentrations if desired. In addition to the new VFA sensor system, test results from development and testing of the in situ...

  19. Report on the Audit of Unattended Ground Sensor Systems

    Science.gov (United States)

    1991-02-26

    This final report on the Audit of Unattended Ground Sensor Systems is for your information and use. Comments on the draft were considered in...preparing the final report and changes have been made where appropriate. We performed the audit from February through August 1990. The objective was to

  20. Simulator of a fail detector system for redundant sensors

    International Nuclear Information System (INIS)

    Assumpcao Filho, E.O.; Nakata, H.

    1990-01-01

    A failure detection and isolation system (FDI) simulation program has been developed for IBM-PC microcomputers. The program, based on the sequencial likelihood ratio testing method developed by A. Wald, was implemented with Monte-Carlo technique. The calculated failure detection rate was favorably compared against the wind-tunnel experimental redundant temperature sensors. (author)

  1. Applications of Miniaturized Atomic Magnetic Sensors in Military Systems

    Science.gov (United States)

    2012-10-01

    system - sensors are obscured under traffic cones and laid out in an array depending on the protection scenario; The Operator Control Station tablet PC...T., Peters, M., Sandin, H., Savukov, I., Schultz, L., Urbatis, A., Volegov, P., and Zotev, V., 2010, Ultra-low-field MRI for the detection of

  2. A Multiple Sensor Machine Vision System Technology for the Hardwood

    Science.gov (United States)

    Richard W. Conners; D.Earl Kline; Philip A. Araman

    1995-01-01

    For the last few years the authors have been extolling the virtues of a multiple sensor approach to hardwood defect detection. Since 1989 the authors have actively been trying to develop such a system. This paper details some of the successes and failures that have been experienced to date. It also discusses what remains to be done and gives time lines for the...

  3. Diameter sensors for tree-length harvesting systems

    Science.gov (United States)

    T.P. McDonald; Robert B. Rummer; T.E. Grift

    2003-01-01

    Most cut-to-length (CTL) harvesters provide sensors for measuring diameter of trees as they are cut and processed. Among other uses, this capability provides a data collection tool for marketing of logs in real time. Logs can be sorted and stacked based on up-to-date market information, then transportation systems optimized to route wood to proper destinations at...

  4. Mining sensor data from complex systems

    NARCIS (Netherlands)

    Vespier, Ugo

    2015-01-01

    Today, virtually everything, from natural phenomena to complex artificial and physical systems, can be measured and the resulting information collected, stored and analyzed in order to gain new insight. This thesis shows how complex systems often exhibit diverse behavior at different temporal

  5. Design of Early Warning System Based on Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Gan Bo

    2018-01-01

    Full Text Available In order to solve the shortcomings of the landslide monitoring technology method, a set of landslides monitoring and early warning system is designed. It can achieve real-time sensor data acquisition, remote transmission and query display. In addition, aiming at the harsh environment of landslide monitoring and the performance requirements of the monitoring system, an improved minimum hop routing protocol is proposed. It can reduce network energy consumption, enhance network robustness, and improve node layout and networking flexibility. In order to realize the remote transmission of data, GPRS wireless communication is used to transmit monitoring data. Combined with remote monitoring center, real-time data display, query, preservation and landslide warning and prediction are realized. The results show that the sensor data acquisition system is accurate, the system is stable, and the node network is flexible. Therefore, the monitoring system has a good use value.

  6. A multichannel portable ECG system with capacitive sensors

    International Nuclear Information System (INIS)

    Oehler, M; Schilling, M; Ling, V; Melhorn, K

    2008-01-01

    Capacitive sensors can be employed for measuring the electrocardiogram of a human heart without electric contact with the skin. This configuration avoids contact problems experienced by conventional electrocardiography. In our studies, we integrated these capacitive electrocardiogram electrodes in a 15-sensor array and combined this array with a tablet personal computer. By placing the system on the patient's body, we can measure a 15-channel electrocardiogram even through clothes and without any preparation. The goal of this development is to provide a new diagnostic tool that offers the user a reproducible, easy access to a fast and spatially resolved diagnostic 'heart view'

  7. Optimal Sensor and Actuator Location for Unstable Systems

    DEFF Research Database (Denmark)

    Shaker, Hamid Reza; Tahavori, Maryamsadat

    2013-01-01

    on the processes. Dually the problem of placing actuators on the processes is equally important. In this paper, the problem of determining optimal sensor and actuator locations for the linear systems is addressed. The problem of the sensor locations is viewed as the problem of maximizing the output energy...... generated by a given state and for the actuator locations is viewed as the problem of minimizing the input energy required to reach a given state. Such design problems occur in many applications, and therefore have been studied extensively. Unfortunately, the results in this context, which have been...

  8. Effect of sensor systems for cow management on milk production, somatic cell count, and reproduction.

    Science.gov (United States)

    Steeneveld, W; Vernooij, J C M; Hogeveen, H

    2015-06-01

    To improve management on dairy herds, sensor systems have been developed that can measure physiological, behavioral, and production indicators on individual cows. It is not known whether using sensor systems also improves measures of health and production in dairy herds. The objective of this study was to investigate the effect of using sensor systems on measures of health and production in dairy herds. Data of 414 Dutch dairy farms with (n=152) and without (n=262) sensor systems were available. For these herds, information on milk production per cow, days to first service, first calving age, and somatic cell count (SCC) was provided for the years 2003 to 2013. Moreover, year of investment in sensor systems was available. For every farm year, we determined whether that year was before or after the year of investment in sensor systems on farms with an automatic milking system (AMS) or a conventional milking system (CMS), or whether it was a year on a farm that never invested in sensor systems. Separate statistical analyses were performed to determine the effect of sensor systems for mastitis detection (color, SCC, electrical conductivity, and lactate dehydrogenase sensors), estrus detection for dairy cows, estrus detection for young stock, and other sensor systems (weighing platform, rumination time sensor, fat and protein sensor, temperature sensor, milk temperature sensor, urea sensor, β-hydroxybutyrate sensor, and other sensor systems). The AMS farms had a higher average SCC (by 12,000 cells/mL) after sensor investment, and CMS farms with a mastitis detection system had a lower average SCC (by 10,000 cells/mL) in the years after sensor investment. Having sensor systems was associated with a higher average production per cow on AMS farms, and with a lower average production per cow on CMS farms in the years after investment. The most likely reason for this lower milk production after investment was that on 96% of CMS farms, the sensor system investment occurred

  9. System overview and applications of a panoramic imaging perimeter sensor

    International Nuclear Information System (INIS)

    Pritchard, D.A.

    1995-01-01

    This paper presents an overview of the design and potential applications of a 360-degree scanning, multi-spectral intrusion detection sensor. This moderate-resolution, true panoramic imaging sensor is intended for exterior use at ranges from 50 to 1,500 meters. This Advanced Exterior Sensor (AES) simultaneously uses three sensing technologies (infrared, visible, and radar) along with advanced data processing methods to provide low false-alarm intrusion detection, tracking, and immediate visual assessment. The images from the infrared and visible detector sets and the radar range data are updated as the sensors rotate once per second. The radar provides range data with one-meter resolution. This sensor has been designed for easy use and rapid deployment to cover wide areas beyond or in place of typical perimeters, and tactical applications around fixed or temporary high-value assets. AES prototypes are in development. Applications discussed in this paper include replacements, augmentations, or new installations at fixed sites where topological features, atmospheric conditions, environmental restrictions, ecological regulations, and archaeological features limit the use of conventional security components and systems

  10. Sensors for advanced driver assistance systems; Sensoren fuer Fahrerassistenzsysteme

    Energy Technology Data Exchange (ETDEWEB)

    Ritschel, W.; Wixforth, T. [Hella KGaA Hueck und Co., Lippstadt (Germany)

    2004-07-01

    Essential safety applications and those aimed at driver convenience (blind spot surveillance, stop and go, pre-crash, parking assistant) can be effected in vehicles with the aid of radar sensors. The radar sensors used can be differentiated in terms of the bandwidth required (narrow band or ultra-wide band) and in terms of the modulation of the transmission signal (pulse modulation or CW). Ultra-wide band systems at the moment are not eligible for admission and do not conform with the present regulations in the European Union. The sensors currently being developed at hella for production use are characterized by the fact that they cover the primary applications in motor vehicles. In these cases the transmission signals radiated lie within the valid limits currently approved within the European Union. (orig.)

  11. Miniature fiber Bragg grating sensor interrogator (FBG-Transceiver) system

    Science.gov (United States)

    Mendoza, Edgar A.; Kempen, Cornelia; Lopatin, Craig

    2007-04-01

    This paper describes recent progress conducted towards the development of a miniature fiber Bragg grating sensor interrogator (FBG-Transceiver TM) system based on multi-channel integrated optic sensor (InOSense TM) microchip technology. The hybrid InOSense TM microchip technology enables the integration of all of the functionalities, both passive and active, of conventional bench top FBG sensor interrogator systems, packaged in a miniaturized, low power operation, 2-cm x 5-cm package suitable for the long-term structural health monitoring in applications where size, weight, and power are critical for operation. The FBG-Transceiver system uses active optoelectronic components monolithically integrated to the InOSense TM microchip, a microprocessor controlled signal processing electronics board capable of processing the FBG sensors signals related to stress-strain and temperature as well as vibration and acoustics. The FBG-Transceiver TM system represents a new, reliable, highly robust technology that can be used to accurately monitor the status of an array of distributed fiber optic Bragg grating sensors installed in critical infrastructures. Its miniature package, low power operation, and state-of-the-art data communications architecture, all at a very affordable price makes it a very attractive solution for a large number of SHM/NDI applications in aerospace, naval and maritime industry, civil structures like bridges, buildings and dams, the oil and chemical industry, and for homeland security applications. The miniature, cost-efficient FBG-Transceiver TM system is poised to revolutionize the field of structural health monitoring and nondestructive inspection market. The sponsor of this program is NAVAIR under a DOD SBIR contract.

  12. MEMS CHIP CO2 SENSOR FOR BUILDING SYSTEMS INTEGRATION

    Energy Technology Data Exchange (ETDEWEB)

    Anton Carl Greenwald

    2005-09-14

    The objective of this research was to develop an affordable, reliable sensor to enable demand controlled ventilation (DCV). A significant portion of total energy consumption in the United States is used for heating or air conditioning (HVAC) buildings. To assure occupant safety and fresh air levels in large buildings, and especially those with sealed windows, HVAC systems are frequently run in excess of true requirements as automated systems cannot now tell the occupancy level of interior spaces. If such a sensor (e.g. thermostat sized device) were available, it would reduce energy use between 10 and 20% in such buildings. A quantitative measure of ''fresh air'' is the concentration of carbon dioxide (CO{sub 2}) present. An inert gas, CO{sub 2} is not easily detected by chemical sensors and is usually measured by infrared spectroscopy. Ion Optics research developed a complete infrared sensor package on a single MEMS chip. It contains the infrared (IR) source, IR detector and IR filter. The device resulting from this DOE sponsored research has sufficient sensitivity, lifetime, and drift rate to meet the specifications of commercial instrument manufacturers who are now testing the device for use in their building systems.

  13. Optical Imaging Sensors and Systems for Homeland Security Applications

    CERN Document Server

    Javidi, Bahram

    2006-01-01

    Optical and photonic systems and devices have significant potential for homeland security. Optical Imaging Sensors and Systems for Homeland Security Applications presents original and significant technical contributions from leaders of industry, government, and academia in the field of optical and photonic sensors, systems and devices for detection, identification, prevention, sensing, security, verification and anti-counterfeiting. The chapters have recent and technically significant results, ample illustrations, figures, and key references. This book is intended for engineers and scientists in the relevant fields, graduate students, industry managers, university professors, government managers, and policy makers. Advanced Sciences and Technologies for Security Applications focuses on research monographs in the areas of -Recognition and identification (including optical imaging, biometrics, authentication, verification, and smart surveillance systems) -Biological and chemical threat detection (including bios...

  14. WIRELESS SENSOR SYSTEM FOR IMPLEMENTATION OF SMART SPACES

    Directory of Open Access Journals (Sweden)

    Gerardo Cázarez-Ayala

    2014-01-01

    Full Text Available This paper describes the design, implementation and application of a smart sensor system based in wireless communication protocol, which was developed with the main objective of facilitate the implementation of smart places, whereby monitoring and supervision of environmental physical variables in a residence or commercial buildings. Based in this system, we want to co-help taking advantage and save electric energy, optimizing the use of the lighting systems and air conditioner only in the schedules and under pre-established conditions for the final user. The system is based in a variety of nodes o modules of sensors like temperature, humidity, light, carbon monoxide, noise and LP gas which have the ability to work collaboratively in networks with topologies like star, tree and mesh.

  15. A Class of Two-Component Adler—Bobenko—Suris Lattice Equations

    International Nuclear Information System (INIS)

    Fu Wei; Zhang Da-Jun; Zhou Ru-Guang

    2014-01-01

    We study a class of two-component forms of the famous list of the Adler—Bobenko—Suris lattice equations. The obtained two-component lattice equations are still consistent around the cube and they admit solutions with ‘jumping properties’ between two levels. (general)

  16. Review of High-Speed Fiber Optic Grating Sensors Systems

    Energy Technology Data Exchange (ETDEWEB)

    Udd, E; Benterou, J; May, C; Mihailov, S J; Lu, P

    2010-03-24

    Fiber grating sensors can be used to support a wide variety of high speed measurement applications. This includes measurements of vibrations on bridges, traffic monitoring on freeways, ultrasonic detection to support non-destructive tests on metal plates and providing details of detonation events. This paper provides a brief overview of some of the techniques that have been used to support high speed measurements using fiber grating sensors over frequency ranges from 10s of kHz, to MHZ and finally toward frequencies approaching the GHz regime. Very early in the development of fiber grating sensor systems it was realized that a high speed fiber grating sensor system could be realized by placing an optical filter that might be a fiber grating in front of a detector so that spectral changes in the reflection from a fiber grating were amplitude modulated. In principal the only limitation on this type of system involved the speed of the output detector which with the development of high speed communication links moved from the regime of 10s of MHz toward 10s of GHz. The earliest deployed systems involved civil structures including measurements of the strain fields on composite utility poles and missile bodies during break tests, bridges and freeways. This was followed by a series of developments that included high speed fiber grating sensors to support nondestructive testing via ultrasonic wave detection, high speed machining and monitoring ship hulls. Each of these applications involved monitoring mechanical motion of structures and thus interest was in speeds up to a few 10s of MHz. Most recently there has been interest in using fiber grating to monitor the very high speed events such as detonations and this has led to utilization of fiber gratings that are consumed during an event that may require detection speeds of hundreds of MHz and in the future multiple GHz.

  17. 3D sensors and micro-fabricated detector systems

    International Nuclear Information System (INIS)

    Da Vià, Cinzia

    2014-01-01

    Micro-systems based on the Micro Electro Mechanical Systems (MEMS) technology have been used in miniaturized low power and low mass smart structures in medicine, biology and space applications. Recently similar features found their way inside high energy physics with applications in vertex detectors for high-luminosity LHC Upgrades, with 3D sensors, 3D integration and efficient power management using silicon micro-channel cooling. This paper reports on the state of this development

  18. Locomotive monitoring system using wireless sensor networks

    CSIR Research Space (South Africa)

    Croucamp, PL

    2014-07-01

    Full Text Available Theft of cables used for powering a locomotive not only stops the train from functioning but also paralyzes the signalling and monitoring system. This means that information on certain locomotive's cannot be passed onto other locomotives which may...

  19. Portable DMFC system with methanol sensor-less control

    Energy Technology Data Exchange (ETDEWEB)

    Chen, C.Y.; Liu, D.H.; Huang, C.L.; Chang, C.L. [Institute of Nuclear Energy Research (INER), No. 1000, Wunhua Rd., Jiaan Village, Longtan Township, Taoyuan County 32546 (China)

    2007-05-15

    This work develops a prototype 20 W portable DMFC by system integration of stack, condenser, methanol sensor-less control and start-up characteristics. The effects of these key components and control schemes on the performance are also discussed. To expedite the use of portable DMFC in electronic applications, the system utilizes a novel methanol sensor-less control method, providing improved fuel efficiency, durability, miniaturization and cost reduction. The operating characteristics of the DMFC stack are applied to control the fuel ejection time and period, enabling the system to continue operating even when the MEAs of the stack are deteriorated. The portable system is also designed with several features including water balance and quick start-up (in 5 min). Notably, the proposed system using methanol sensor-less control with injection of pure methanol can power the DVD player and notebook PC. The system specific energy and energy density following three days of operation are 362 Wh kg{sup -1} and 335 Wh L{sup -1}, respectively, which are better than those of lithium batteries ({proportional_to}150 Wh kg{sup -1} and {proportional_to}250 Wh L{sup -}). This good energy storage feature demonstrates that the portable DMFC is likely to be valuable in computer, communication and consumer electronic (3C) markets. (author)

  20. Wide Band Low Noise Love Wave Magnetic Field Sensor System.

    Science.gov (United States)

    Kittmann, Anne; Durdaut, Phillip; Zabel, Sebastian; Reermann, Jens; Schmalz, Julius; Spetzler, Benjamin; Meyners, Dirk; Sun, Nian X; McCord, Jeffrey; Gerken, Martina; Schmidt, Gerhard; Höft, Michael; Knöchel, Reinhard; Faupel, Franz; Quandt, Eckhard

    2018-01-10

    We present a comprehensive study of a magnetic sensor system that benefits from a new technique to substantially increase the magnetoelastic coupling of surface acoustic waves (SAW). The device uses shear horizontal acoustic surface waves that are guided by a fused silica layer with an amorphous magnetostrictive FeCoSiB thin film on top. The velocity of these so-called Love waves follows the magnetoelastically-induced changes of the shear modulus according to the magnetic field present. The SAW sensor is operated in a delay line configuration at approximately 150 MHz and translates the magnetic field to a time delay and a related phase shift. The fundamentals of this sensor concept are motivated by magnetic and mechanical simulations. They are experimentally verified using customized low-noise readout electronics. With an extremely low magnetic noise level of ≈100 pT/[Formula: see text], a bandwidth of 50 kHz and a dynamic range of 120 dB, this magnetic field sensor system shows outstanding characteristics. A range of additional measures to further increase the sensitivity are investigated with simulations.

  1. Printable low-cost sensor systems for healthcare smart textiles

    Science.gov (United States)

    Rai, Pratyush; Kumar, Prashanth S.; Oh, Sechang; Kwon, Hyeokjun; Mathur, Gyanesh N.; Varadan, Vijay K.

    2011-04-01

    Smart textiles-based wearable health monitoring systems (ST-HMS) have been presented as elegant solutions to the requirements of individuals across a wide range of ages. They can be used to monitor young or elderly recuperating /convalescent patients either in hospital or at home, or they can be used by young athletes to monitor important physiological parameters to better design their training or fitness program. Business and academic interests, all over the world, have fueled a great deal of work in the development of this technology since 1990. However, two important impediments to the development of ST-HMS are:-integration of flexible electrodes, flexible sensors, signal conditioning circuits and data logging or wireless transmission devices into a seamless garment and a means to mass manufacture the same, while keeping the costs low. Roll-to-roll printing and screen printing are two low cost methods for large scale manufacturing on flexible substrates and can be extended to textiles as well. These two methods are, currently, best suited for planar structures. The sensors, integrated with wireless telemetry, facilitate development of a ST-HMS that allows for unobtrusive health monitoring. In this paper, we present our results with planar screen printable sensors based on conductive inks which can be used to monitor EKG, abdominal respiration effort, blood pressure, pulse rate and body temperature. The sensor systems were calibrated, and tested for sensitivity, reliability and robustness to ensure reuse after washing cycles.

  2. EMI free fiber optic strain sensor system for TFTR

    International Nuclear Information System (INIS)

    Szuchy, N.C.; Caserta, A.L.; Ferrara, A.A.; Squires, R.W.; Sredniawski, J.J.

    1983-01-01

    In certain applications, structural components are subjected to loadings in high electromagnetic interference (EMI) environments. The mechanical responses of these components must be monitored under rapidly varying electromagnetic fields. A Fiber Optic Strain Sensor System (FOSSS) is an acceptable solution since it is immune to EMI. Grumman Aerospace Corporation initiated the development of a FOSSS that can be used in high EMI situations where resistive/electronic-based strain measurement systems would not be effective, such as on the Tokamak Fusion Test Reactor (TFTR) during plasma disruption. Tests have indicated that because of their increased sensitivity due to the size of the fiber optic (FO) transducer (1-in. 2 ) and responsiveness due to the areal changes of the FO sensor, the strain tracking capability of FO sensors are excellent. For the TFTR application a jacketed 400-micron fiber capable of operating in a 250 0 C temperature environment was used. Continuous 30 foot lengths of high-temperature FO cables were affixed to 304 LN SS tabs, forming an integrated strain sensor and pigtail unit. By fusion splicing 400-micron room temperature fibers to the pigtails, the required runs (approximately 200 feet) to the TFTR data acquisition room were made with minimum coupling attenuation. Development methodology is discussed and test data presented

  3. Selected examples of intelligent (micro) sensor systems: state-of-the-art and tendencies

    Science.gov (United States)

    Hauptmann, Peter R.

    2006-03-01

    The capability of intelligent sensors to have more intelligence built into them continues to drive their application in areas including automotive, aerospace and defense, industrial, intelligent house and wear, medical and homeland security. In principle it is difficult to overestimate the importance of intelligent (micro) sensors or sensor systems within advanced societies but one characteristic feature is the global market for sensors, which is now about 20 billion annually. Therefore sensors or sensor systems play a dominant role in many fields from the macro sensor in manufacturing industry down to the miniaturized sensor for medical applications. The diversity of sensors precludes a complete description of the state-of-the-art; selected examples will illustrate the current situation. MEMS (microelectromechanical systems) devices are of special interest in the context of micro sensor systems. In past the main requirements of a sensor were in terms of metrological performance. The electrical (or optical) signal produced by the sensor needed to match the measure relatively accurately. Such basic functionality is no longer sufficient. Data processing near the sensor, the extraction of more information than just the direct sensor information by signal analysis, system aspects and multi-sensor information are the new demands. A shifting can be observed away from aiming to design perfect single-function transducers and towards the utilization of system-based sensors as system components. In the ideal case such systems contain sensors, actuators and electronics. They can be realized in monolithic, hybrid or discrete form—which kind is used depends on the application. In this article the state-of-the-art of intelligent sensors or sensor systems is reviewed using selected examples. Future trends are deduced.

  4. Gait Dynamics Sensing Using IMU Sensor Array System

    Directory of Open Access Journals (Sweden)

    Slavomir Kardos

    2017-01-01

    Full Text Available The article deals with a progressive approach in gait sensing. It is incorporated by IMU (Inertia Measurement Unit complex sensors whose field of acting is mainly the motion sensing in medicine, automotive and other industry, self-balancing systems, etc. They allow acquiring the position and orientation of an object in 3D space. Using several IMU units the sensing array for gait dynamics was made. Based on human gait analysis the 7-sensor array was designed to build a gait motion dynamics sensing system with the possibility of graphical interpretation of data from the sensing modules in real-time graphical application interface under the LabVIEW platform. The results of analyses can serve as the information for medical diagnostic purposes. The main control part of the system is microcontroller, whose function is to control the data collection and flow, provide the communication and power management.

  5. Power consumption analysis of operating systems for wireless sensor networks.

    Science.gov (United States)

    Lajara, Rafael; Pelegrí-Sebastiá, José; Perez Solano, Juan J

    2010-01-01

    In this paper four wireless sensor network operating systems are compared in terms of power consumption. The analysis takes into account the most common operating systems--TinyOS v1.0, TinyOS v2.0, Mantis and Contiki--running on Tmote Sky and MICAz devices. With the objective of ensuring a fair evaluation, a benchmark composed of four applications has been developed, covering the most typical tasks that a Wireless Sensor Network performs. The results show the instant and average current consumption of the devices during the execution of these applications. The experimental measurements provide a good insight into the power mode in which the device components are running at every moment, and they can be used to compare the performance of different operating systems executing the same tasks.

  6. Measurement system for nitrous oxide based on amperometric gas sensor

    Science.gov (United States)

    Siswoyo, S.; Persaud, K. C.; Phillips, V. R.; Sneath, R.

    2017-03-01

    It has been well known that nitrous oxide is an important greenhouse gas, so monitoring and control of its concentration and emission is very important. In this work a nitrous oxide measurement system has been developed consisting of an amperometric sensor and an appropriate lab-made potentiostat that capable measuring picoampere current ranges. The sensor was constructed using a gold microelectrode as working electrode surrounded by a silver wire as quasi reference electrode, with tetraethyl ammonium perchlorate and dimethylsulphoxide as supporting electrolyte and solvent respectively. The lab-made potentiostat was built incorporating a transimpedance amplifier capable of picoampere measurements. This also incorporated a microcontroller based data acquisition system, controlled by a host personal computer using a dedicated computer program. The system was capable of detecting N2O concentrations down to 0.07 % v/v.

  7. EXPERIMENTAL SENSOR OF THE BENZOETHANOL COMPOSITION FOR ENGINE FUEL SYSTEM

    Directory of Open Access Journals (Sweden)

    V. Bgantsev

    2017-12-01

    Full Text Available An important aspect of the economy of internal combustion engine on benzoethanol is the accuracy of regulation of the fuel-air mixture composition. This task is complicated by fluctuations in the composition of benzoethanol, depending on the refueling of the vehicle at various filling stations. In this connection, there is a need to control the composition of benzoethanol in the fuel system of the engine and adjust the fuel supply system. With this purpose, fuel systems are equipped with special sensors that generate a signal, depending on the alcohol content of the mixed fuel. In the article one of the design solutions of the experimental sensor of the benzoethanol composition and the results of its testing with fuels of various composition are given.

  8. Fiber optic sensor system for entrance areas monitoring

    Science.gov (United States)

    Fajkus, Marcel; Nedoma, Jan; Kepak, Stanislav; Cubik, Jakub; Jargus, Jan; Zboril, Ondřej; Martinek, Radek; Vasinek, Vladimir

    2017-10-01

    Authors of this article present the fiber-optic system based on fiber Bragg gratings (FBGs) which are used to secure the entrance areas such as buildings, halls, warehouses, etc. The system uses the specially encapsulated sensory array of fiber Bragg gratings which are implemented into the floor or on the floor and allows for monitoring the area of 1 m2 up to 100 m2 depending on the number of FBG sensors. The sensory array is characterized by immunity to electromagnetic interference (EMI), passivity regarding electrical power supply, the possibility of remote evaluation (up to units of km) and high sensitivity. Proposed sensor system has detection capability greater than 99 % and furthermore, provides information about the weight load to an accuracy of +/- 5 kg. The concept has been tested in a real environment within the test polygon for several weeks. As the reference devices, we used the CCTV (Closed Circuit Television).

  9. Unmanned Aircraft Systems (UAS) Sensor and Targeting

    Science.gov (United States)

    2010-07-27

    Identify an uncoiled garden hose lying on the ground. 8.2 Identify specific type of truck crop being grown (e.g., tomatoes, peppers, lettuce ). 8.2... lettuce ). (NIIRS 8.2) Detect scoring of poppy bulbs. (NIIRS 8.5) Detect tubing (approximately 1-inch diameter) for drip irrigation systems. (NIIR5 8.5...trial can be presented as a time history for each axis. A sample is shown in Figures C.1-3 and C.1-4. TOP 07-1-003 27 July 2010 C-2

  10. Quantum-phase dynamics of two-component Bose-Einstein condensates: Collapse-revival of macroscopic superposition states

    International Nuclear Information System (INIS)

    Nakano, Masayoshi; Kishi, Ryohei; Ohta, Suguru; Takahashi, Hideaki; Furukawa, Shin-ichi; Yamaguchi, Kizashi

    2005-01-01

    We investigate the long-time dynamics of two-component dilute gas Bose-Einstein condensates with relatively different two-body interactions and Josephson couplings between the two components. Although in certain parameter regimes the quantum state of the system is known to evolve into macroscopic superposition, i.e., Schroedinger cat state, of two states with relative atom number differences between the two components, the Schroedinger cat state is also found to repeat the collapse and revival behavior in the long-time region. The dynamical behavior of the Pegg-Barnett phase difference between the two components is shown to be closely connected with the dynamics of the relative atom number difference for different parameters. The variation in the relative magnitude between the Josephson coupling and intra- and inter-component two-body interaction difference turns out to significantly change not only the size of the Schroedinger cat state but also its collapse-revival period, i.e., the lifetime of the Schroedinger cat state

  11. A sensor-based automation system for handling nuclear materials

    International Nuclear Information System (INIS)

    Drotning, W.; Kimberly, H.; Wapman, W.; Darras, D.

    1997-01-01

    An automated system is being developed for handling large payloads of radioactive nuclear materials in an analytical laboratory. The automation system performs unpacking and repacking of payloads from shipping and storage containers, and delivery of the payloads to the stations in the laboratory. The system uses machine vision and force/torque sensing to provide sensor-based control of the automation system in order to enhance system safety, flexibility, and robustness, and achieve easy remote operation. The automation system also controls the operation of the laboratory measurement systems and the coordination of them with the robotic system. Particular attention has been given to system design features and analytical methods that provide an enhanced level of operational safety. Independent mechanical gripper interlock and tool release mechanisms were designed to prevent payload mishandling. An extensive Failure Modes and Effects Analysis of the automation system was developed as a safety design analysis tool

  12. Multi-sensor explosive detection system

    International Nuclear Information System (INIS)

    Gozani, T.; Shea, P.M.; Sawa, Z.P.

    1992-01-01

    This patent describes an explosive detection system. It comprises a source of neutrons; a detector array comprising a plurality of gamma ray detectors, each of the gamma ray detectors providing a detection signal in the event a gamma ray is captured by the detector, and at least one neutron detector, the neutron detector providing a neutron detection signal in the event a neutron is captured by the neutron detector; means for irradiating an object being examined with neutrons from the neutron source and for positioning the detector array relative to the object so that gamma rays emitted from the elements within the object as a result of the neutron irradiation are detected by the gamma ray detectors of the detector array; and parallel distributed processing means responsive to the detection signals of the detector array for discriminating between objects carrying explosives and objects not carrying explosives, the parallel distributed processing means including an artificial neural system (ANS), the ANS having a parallel network of processors, each processor of the parallel network of processors, each processor of the parallel network of processors including means for receiving at least one input signal, and means for generating an output signal as a function of the at least one input signal

  13. Finger and foot tapping sensor system for objective motor assessment

    Directory of Open Access Journals (Sweden)

    Đurić-Jovičić Milica

    2018-01-01

    Full Text Available Background/Aim. Finger tapping test is commonly used in neurological examinations as a test of motor performance. The new system comprising inertial and force sensors and custom proprietary software was developed for quantitative estimation and assessment of finger and foot tapping tests. The aim of this system was to provide diagnosis support and objective assessment of motor function. Methods. Miniature inertial sensors were placed on fingertips and used for measuring finger movements. A force sensor was placed on the fingertip of one finger, in order to measure the force during tapping. For foot tapping assessment, an inertial sensor was mounted on the subject’s foot, which was placed above a force platform. By using this system, various parameters such as a number of taps, tapping duration, rhythm, open and close speed, the applied force and tapping angle, can be extracted for detailed analysis of a patient’s motor performance. The system was tested on 13 patients with Parkinson’s disease and 14 healthy controls. Results. The system allowed easy measurement of listed parameters, and additional graphical representation showed quantitative differences in these parameters between neurological patient and healthy subjects. Conclusion. The novel system for finger and foot tapping test is compact, simple to use and efficiently collects patient data. Parameters measured in patients can be compared to those measured in healthy subjects, or among groups of patients, or used to monitor progress of the disease, or therapy effects. Created data and scores could be used together with the scores from clinical tests, providing the possibility for better insight into the diagnosis. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175090 and Grant no. 175016

  14. Modeling of a new 2D Acceleration Sensor Array using SystemC-AMS

    International Nuclear Information System (INIS)

    Markert, Erik; Dienel, Marco; Herrmann, Goeran; Mueller, Dietmar; Heinkel, Ulrich

    2006-01-01

    This paper presents an approach for modeling and simulation of a new 2D acceleration sensor array using SystemC-AMS. The sensor array consists of six single acceleration sensors with different detection axes. These single sensors comprise of four capacitive segments and one mass segment, aligned in a semicircle. The redundant sensor information is used for offset correction. Modeling of the single sensors is achieved using sensor structure simplification into 11 points and analytic equations for capacity changes, currents and torques. This model was expanded by a PWM feedback circuit to keep the sensor displacement in a linear region. In this paper the single sensor model is duplicated considering different positions of the seismic mass resulting in different detection axes for the single sensors. The measured accelerations of the sensors are merged with different weights depending on the orientation. This also reduces calculation effort

  15. A Smart Sensor Data Transmission Technique for Logistics and Intelligent Transportation Systems

    OpenAIRE

    Kyunghee Sun; Intae Ryoo

    2018-01-01

    When it comes to Internet of Things systems that include both a logistics system and an intelligent transportation system, a smart sensor is one of the key elements to collect useful information whenever and wherever necessary. This study proposes the Smart Sensor Node Group Management Medium Access Control Scheme designed to group smart sensor devices and collect data from them efficiently. The proposed scheme performs grouping of portable sensor devices connected to a system depending on th...

  16. Ultra-Low Power Sensor System for Disaster Event Detection in Metro Tunnel Systems

    Directory of Open Access Journals (Sweden)

    Jonah VINCKE

    2017-05-01

    Full Text Available In this extended paper, the concept for an ultra-low power wireless sensor network (WSN for underground tunnel systems is presented highlighting the chosen sensors. Its objectives are the detection of emergency events either from natural disasters, such as flooding or fire, or from terrorist attacks using explosives. Earlier works have demonstrated that the power consumption for the communication can be reduced such that the data acquisition (i.e. sensor sub-system becomes the most significant energy consumer. By using ultra-low power components for the smoke detector, a hydrostatic pressure sensor for water ingress detection and a passive acoustic emission sensor for explosion detection, all considered threats are covered while the energy consumption can be kept very low in relation to the data acquisition. In addition to 1 the sensor system is integrated into a sensor board. The total average power consumption for operating the sensor sub-system is measured to be 35.9 µW for lower and 7.8 µW for upper nodes.

  17. Images and Spectra of Time Dependent Two Component Advective Flow in Presence of Outflows

    Science.gov (United States)

    Chatterjee, Arka; Chakrabarti, Sandip K.; Ghosh, Himadri; Garain, Sudip K.

    2018-05-01

    Two Component Advective Flow (TCAF) successfully explains the spectral and temporal properties of outbursting or persistent sources. Images of static TCAF with Compton cloud or CENtrifugal pressure supported Boundary Layer (CENBOL) due to gravitational bending of photons have been studied before. In this paper, we study time dependent images of advective flows around a Schwarzschild black hole which include cooling effects due to Comptonization of soft photons from a Keplerian disks well as the self-consistently produced jets and outflows. We show the overall image of the disk-jet system after convolving with a typical beamwidth. A long exposure image with time dependent system need not show the black hole horizon conspicuously, unless one is looking at a soft state with no jet or the system along the jet axis. Assuming these disk-jet configurations are relevant to radio emitting systems also, our results would be useful to look for event horizons in high accretion rate Supermassive Black Holes in Seyfert galaxies, RL Quasars.

  18. Smart Sensors' Role in Integrated System Health Management

    Science.gov (United States)

    Perotti, Jose M.; Mata, Carlos

    2005-01-01

    During the last decade, there has been a major effort in the aerospace industry to reduce the cost per pond of payload and become competitive in the international market. Competition from Europe, Japan, and China has reduced this cost to almost a third from 1990 to 2000. This cost has leveled in recent years to an average price of around $12,000/pound of payload. One of NASA's goals is to promote the development of technologies to reduce this cost by a factor of 10 or more Exploration of space, specially manned exploration missions, involves very complex launch and flight vehicles, associated ground support systems, and extensive human support during all phases of the mission. When considering the Space Shuttle Program, we can see that vehicle and ground support systems' processing, operation, and maintenance represent a large percentage of the program cost and time. Reducing operating, processing and maintenance costs will greatly reduce the cost of Exploration programs. The Integrated System Health Management (ISHM) concept is one of the technologies that will help reduce these operating, processing and maintenance costs. ISHM is an integrated health monitoring system applicable to both flight and ground systems. It automatically and autonomously acquires information from sensors and actuators and processes that information using the ISHM-embedded knowledge. As a result, it establishes the health of the system based on the acquired information and its prior knowledge. When this concept is fully implemented, ISHM systems shall be able to perform failure prediction and remediation before actual hard failures occurs, preventing its costly consequences. Data sources, sensors, and their associated data acquisition systems, constitute the foundation of the system. A smart sensing architecture is required to support the acquisition of reliable, high quality data, required by the ISHM. A thorough definition of the smart sensor architectures, their embedded diagnostic

  19. Power supply for wireless sensor or actuator systems

    International Nuclear Information System (INIS)

    Reindl, L. M.

    2011-01-01

    Portable wireless sensor or actuator systems, like portable phones, remote control, or ID cards play an ever growing role in our industrialized environment. Those systems and many more were enabled due to the steady decreasing power consumption of high integrated ICs. Most such systems are powered by batteries or inductive coupling. In this presentation several concepts for an alternative power supply of wireless sensor or actuator systems are discussed in detail. Batteries, although today mostly used, suffer from a limited storage capacity, which induce a labour and sometimes cost-intensive periodic maintenance, and a problematic ecological impact. The operating range of inductive coupling systems is due to the near ?eld limited to the aperture of the coupling coil. UHF systems operate in the far field and reach higher distances. Their operating range is limited by the distance where the voltage at the feeding point of the antenna becomes too low to drive the rectifier circuit. Larger read out ranges become feasible by omitting the rectifier stage. In this case we need either a passive frequency modulating device to shift the read out signal to a side band, or a resonator with a high quality factor, like a SAW or BAW device, to store the energy until all environmental echoes are feed away. For many applications, both indoor and outdoor, energy harvesting system become feasible which convert ambient power densities like light, RF fields, special or temporal thermal gradients, or mechanical vibrations into electrical supply power of the wireless system. All those systems strongly suffer from a lack of energy. Thus new concepts for low-ering the power consumption of a wireless sensor or actuator system by keeping their features remain extreme important. Herby, a new wake up receiver is presented which operates on a current requirement as low as 3 micro A.

  20. Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB.

    Science.gov (United States)

    Filippova, Ekaterina V; Zemaitaitis, Bozena; Aung, Theint; Wolfe, Alan J; Anderson, Wayne F

    2018-02-27

    RcsB is a highly conserved transcription regulator of the Rcs phosphorelay system, a complex two-component signal transduction system (N. Majdalani and S. Gottesman, Annu Rev Microbiol 59:379-405, 2005; A. J. Wolfe, Curr Opin Microbiol 13:204-209, 2010, https://doi.org/10.1016/j.mib.2010.01.002; D. J. Clarke, Future Microbiol 5:1173-1184, 2010, https://doi.org/10.2217/fmb.10.83). RcsB plays an important role in virulence and pathogenicity in human hosts by regulating biofilm formation. RcsB can regulate transcription alone or together with its auxiliary transcription regulators by forming heterodimers. This complexity allows RcsB to regulate transcription of more than 600 bacterial genes in response to different stresses (D. Wang et al., Mol Plant Microbe Interact 25:6-17, 2012, https://doi.org/10.1094/MPMI-08-11-0207). Despite increasing knowledge of RcsB importance, molecular mechanisms that drive the ability of RcsB to control transcription of a large number of genes remain unclear. Here, we present crystal structures of unphosphorylated RcsB in complex with the consensus DNA-binding sequence of 22-mer (DNA22) and 18-mer (DNA18) of the flhDC operon from Escherichia coli determined at 3.15- and 3.37-Å resolution, respectively. The results of our structural analysis combined with the results of in vitro binding assays provide valuable insights to the protein regulatory mechanism, demonstrate how RcsB recognizes target DNA sequences, and reveal a unique oligomeric state that allows RcsB to form homo- and heterodimers. This information will help us understand the complex mechanisms of transcriptional regulation by RcsB in bacteria. IMPORTANCE RcsB is a well-studied two-component response regulator of the Rcs phosphorelay system, conserved within the family Enterobacteriaceae , which includes many pathogens. It is a global regulator, controlling more than 5% of bacterial genes associated with capsule biosynthesis, flagellar biogenesis, cell wall biosynthesis

  1. 78 FR 17187 - Notice of Intent To Grant Exclusive Patent License; Fiber Optic Sensor Systems Technology...

    Science.gov (United States)

    2013-03-20

    ...; Fiber Optic Sensor Systems Technology Corporation AGENCY: Department of the Navy, DoD. ACTION: Notice..., 2012, announcing an intent to grant to Fiber Optic Sensor Systems Technology Corporation, a revocable... the Navy hereby gives notice of its intent to grant to Fiber Optic Sensor Systems Technology...

  2. Active Wireless Temperature Sensors for Aerospace Thermal Protection Systems

    Science.gov (United States)

    Milos, Frank S.; Karunaratne, K.; Arnold, Jim (Technical Monitor)

    2002-01-01

    Health diagnostics is an area where major improvements have been identified for potential implementation into the design of new reusable launch vehicles in order to reduce life-cycle costs, to increase safety margins, and to improve mission reliability. NASA Ames is leading the effort to advance inspection and health management technologies for thermal protection systems. This paper summarizes a joint project between NASA Ames and Korteks to develop active wireless sensors that can be embedded in the thermal protection system to monitor sub-surface temperature histories. These devices are thermocouples integrated with radio-frequency identification circuitry to enable acquisition and non-contact communication of temperature data through aerospace thermal protection materials. Two generations of prototype sensors are discussed. The advanced prototype collects data from three type-k thermocouples attached to a 2.54-cm square integrated circuit.

  3. Neuromorphic vision sensors and preprocessors in system applications

    Science.gov (United States)

    Kramer, Joerg; Indiveri, Giacomo

    1998-09-01

    A partial review of neuromorphic vision sensors that are suitable for use in autonomous systems is presented. Interfaces are being developed to multiplex the high- dimensional output signals of arrays of such sensors and to communicate them in standard formats to off-chip devices for higher-level processing, actuation, storage and display. Alternatively, on-chip processing stages may be implemented to extract sparse image parameters, thereby obviating the need for multiplexing. Autonomous robots are used to test neuromorphic vision chips in real-world environments and to explore the possibilities of data fusion from different sensing modalities. Examples of autonomous mobile systems that use neuromorphic vision chips for line tracking and optical flow matching are described.

  4. A Printed Organic Circuit System for Wearable Amperometric Electrochemical Sensors.

    Science.gov (United States)

    Shiwaku, Rei; Matsui, Hiroyuki; Nagamine, Kuniaki; Uematsu, Mayu; Mano, Taisei; Maruyama, Yuki; Nomura, Ayako; Tsuchiya, Kazuhiko; Hayasaka, Kazuma; Takeda, Yasunori; Fukuda, Takashi; Kumaki, Daisuke; Tokito, Shizuo

    2018-04-23

    Wearable sensor device technologies, which enable continuous monitoring of biological information from the human body, are promising in the fields of sports, healthcare, and medical applications. Further thinness, light weight, flexibility and low-cost are significant requirements for making the devices attachable onto human tissues or clothes like a patch. Here we demonstrate a flexible and printed circuit system consisting of an enzyme-based amperometric sensor, feedback control and amplification circuits based on organic thin-film transistors. The feedback control and amplification circuits based on pseudo-CMOS inverters were successfuly integrated by printing methods on a plastic film. This simple system worked very well like a potentiostat for electrochemical measurements, and enabled the quantitative and real-time measurement of lactate concentration with high sensitivity of 1 V/mM and a short response time of a hundred seconds.

  5. Airborne Wireless Sensor Networks for Airplane Monitoring System

    Directory of Open Access Journals (Sweden)

    Shang Gao

    2018-01-01

    Full Text Available In traditional airplane monitoring system (AMS, data sensed from strain, vibration, ultrasound of structures or temperature, and humidity in cabin environment are transmitted to central data repository via wires. However, drawbacks still exist in wired AMS such as expensive installation and maintenance, and complicated wired connections. In recent years, accumulating interest has been drawn to performing AMS via airborne wireless sensor network (AWSN system with the advantages of flexibility, low cost, and easy deployment. In this review, we present an overview of AMS and AWSN and demonstrate the requirements of AWSN for AMS particularly. Furthermore, existing wireless hardware prototypes and network communication schemes of AWSN are investigated according to these requirements. This paper will improve the understanding of how the AWSN design under AMS acquires sensor data accurately and carries out network communication efficiently, providing insights into prognostics and health management (PHM for AMS in future.

  6. Onsager Vortex Formation in Two-component Bose-Einstein Condensates

    Science.gov (United States)

    Han, Junsik; Tsubota, Makoto

    2018-06-01

    We numerically study the dynamics of quantized vortices in two-dimensional two-component Bose-Einstein condensates (BECs) trapped by a box potential. For one-component BECs in a box potential, it is known that quantized vortices form Onsager vortices, which are clusters of same-sign vortices. We confirm that the vortices of the two components spatially separate from each other — even for miscible two-component BECs — suppressing the formation of Onsager vortices. This phenomenon is caused by the repulsive interaction between vortices belonging to different components, hence, suggesting a new possibility for vortex phase separation.

  7. Weak nonlinear matter waves in a trapped two-component Bose-Einstein condensates

    International Nuclear Information System (INIS)

    Yong Wenmei; Xue Jukui

    2008-01-01

    The dynamics of the weak nonlinear matter solitary waves in two-component Bose-Einstein condensates (BEC) with cigar-shaped external potential are investigated analytically by a perturbation method. In the small amplitude limit, the two-components can be decoupled and the dynamics of solitary waves are governed by a variable-coefficient Korteweg-de Vries (KdV) equation. The reduction to the KdV equation may be useful to understand the dynamics of nonlinear matter waves in two-component BEC. The analytical expressions for the evolution of soliton, emitted radiation profiles and soliton oscillation frequency are also obtained

  8. Microphotonic devices for compact planar lightwave circuits and sensor systems

    Science.gov (United States)

    Cardenas Gonzalez, Jaime

    2005-07-01

    Higher levels of integration in planar lightwave circuits and sensor systems can reduce fabrication costs and broaden viable applications for optical network and sensor systems. For example, increased integration and functionality can lead to sensor systems that are compact enough for easy transport, rugged enough for field applications, and sensitive enough even for laboratory applications. On the other hand, more functional and compact planar lightwave circuits can make optical networks components less expensive for the metro and access markets in urban areas and allow penetration of fiber to the home. Thus, there is an important area of opportunity for increased integration to provide low cost, compact solutions in both network components and sensor systems. In this dissertation, a novel splitting structure for microcantilever deflection detection is introduced. The splitting structure is designed so that its splitting ratio is dependent on the vertical position of the microcantilever. With this structure, microcantilevers sensitized to detect different analytes or biological agents can be integrated into an array on a single chip. Additionally, the integration of a depolarizer into the optoelectronic integrated circuit in an interferometric fiber optic gyroscope is presented as a means for cost reduction. The savings come in avoiding labor intensive fiber pigtailing steps by permitting batch fabrication of these components. In particular, this dissertation focuses on the design of the waveguides and polarization rotator, and the impact of imperfect components on the performance of the depolarizer. In the area of planar lightwave circuits, this dissertation presents the development of a fabrication process for single air interface bends (SAIBs). SAIBs can increase integration by reducing the area necessary to make a waveguide bend. Fabrication and measurement of a 45° SAIB with a bend efficiency of 93.4% for TM polarization and 92.7% for TE polarization are

  9. "Cut-and-Paste" Manufacture of Multiparametric Epidermal Sensor Systems.

    Science.gov (United States)

    Yang, Shixuan; Chen, Ying-Chen; Nicolini, Luke; Pasupathy, Praveenkumar; Sacks, Jacob; Su, Becky; Yang, Russell; Sanchez, Daniel; Chang, Yao-Feng; Wang, Pulin; Schnyer, David; Neikirk, Dean; Lu, Nanshu

    2015-11-04

    Multifunctional epidermal sensor systems (ESS) are manufactured with a highly cost and time effective, benchtop, and large-area "cut-and-paste" method. The ESS made out of thin and stretchable metal and conductive polymer ribbons can be noninvasively laminated onto the skin surface to sense electrophysiological signals, skin temperature, skin hydration, and respiratory rate. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Operating Systems for Wireless Sensor Networks: A Survey

    Directory of Open Access Journals (Sweden)

    Muhammad Omer Farooq

    2011-05-01

    Full Text Available This paper presents a survey on the current state-of-the-art in Wireless Sensor Network (WSN Operating Systems (OSs. In recent years, WSNs have received tremendous attention in the research community, with applications in battlefields, industrial process monitoring, home automation, and environmental monitoring, to name but a few. A WSN is a highly dynamic network because nodes die due to severe environmental conditions and battery power depletion. Furthermore, a WSN is composed of miniaturized motes equipped with scarce resources e.g., limited memory and computational abilities. WSNs invariably operate in an unattended mode and in many scenarios it is impossible to replace sensor motes after deployment, therefore a fundamental objective is to optimize the sensor motes’ life time. These characteristics of WSNs impose additional challenges on OS design for WSN, and consequently, OS design for WSN deviates from traditional OS design. The purpose of this survey is to highlight major concerns pertaining to OS design in WSNs and to point out strengths and weaknesses of contemporary OSs for WSNs, keeping in mind the requirements of emerging WSN applications. The state-of-the-art in operating systems for WSNs has been examined in terms of the OS Architecture, Programming Model, Scheduling, Memory Management and Protection, Communication Protocols, Resource Sharing, Support for Real-Time Applications, and additional features. These features are surveyed for both real-time and non-real-time WSN operating systems.

  11. Operating Systems for Wireless Sensor Networks: A Survey

    Science.gov (United States)

    Farooq, Muhammad Omer; Kunz, Thomas

    2011-01-01

    This paper presents a survey on the current state-of-the-art in Wireless Sensor Network (WSN) Operating Systems (OSs). In recent years, WSNs have received tremendous attention in the research community, with applications in battlefields, industrial process monitoring, home automation, and environmental monitoring, to name but a few. A WSN is a highly dynamic network because nodes die due to severe environmental conditions and battery power depletion. Furthermore, a WSN is composed of miniaturized motes equipped with scarce resources e.g., limited memory and computational abilities. WSNs invariably operate in an unattended mode and in many scenarios it is impossible to replace sensor motes after deployment, therefore a fundamental objective is to optimize the sensor motes’ life time. These characteristics of WSNs impose additional challenges on OS design for WSN, and consequently, OS design for WSN deviates from traditional OS design. The purpose of this survey is to highlight major concerns pertaining to OS design in WSNs and to point out strengths and weaknesses of contemporary OSs for WSNs, keeping in mind the requirements of emerging WSN applications. The state-of-the-art in operating systems for WSNs has been examined in terms of the OS Architecture, Programming Model, Scheduling, Memory Management and Protection, Communication Protocols, Resource Sharing, Support for Real-Time Applications, and additional features. These features are surveyed for both real-time and non-real-time WSN operating systems. PMID:22163934

  12. Operating systems for wireless sensor networks: a survey.

    Science.gov (United States)

    Farooq, Muhammad Omer; Kunz, Thomas

    2011-01-01

    This paper presents a survey on the current state-of-the-art in Wireless Sensor Network (WSN) Operating Systems (OSs). In recent years, WSNs have received tremendous attention in the research community, with applications in battlefields, industrial process monitoring, home automation, and environmental monitoring, to name but a few. A WSN is a highly dynamic network because nodes die due to severe environmental conditions and battery power depletion. Furthermore, a WSN is composed of miniaturized motes equipped with scarce resources e.g., limited memory and computational abilities. WSNs invariably operate in an unattended mode and in many scenarios it is impossible to replace sensor motes after deployment, therefore a fundamental objective is to optimize the sensor motes' life time. These characteristics of WSNs impose additional challenges on OS design for WSN, and consequently, OS design for WSN deviates from traditional OS design. The purpose of this survey is to highlight major concerns pertaining to OS design in WSNs and to point out strengths and weaknesses of contemporary OSs for WSNs, keeping in mind the requirements of emerging WSN applications. The state-of-the-art in operating systems for WSNs has been examined in terms of the OS Architecture, Programming Model, Scheduling, Memory Management and Protection, Communication Protocols, Resource Sharing, Support for Real-Time Applications, and additional features. These features are surveyed for both real-time and non-real-time WSN operating systems.

  13. A Wireless Self-Powered Urinary Incontinence Sensor System

    Science.gov (United States)

    Tanaka, Ami; Utsunomiya, Fumiyasu; Douseki, Takakuni

    A self-powered urinary incontinence sensor system consisting of a urine-activated coin battery and a wireless transmitter has been developed as an application for wireless biosensor networks. The urine-activated battery makes possible both the sensing of urine leakage and self-powered operation. An intermittent power-supply circuit that uses an electric double-layer capacitor (EDLC) with a small internal resistance suppresses the supply voltage drop due to the large internal resistance of the battery. This circuit and a 1-V surface acoustic wave (SAW) oscillator reduce the power dissipation of a wireless transmitter. The SAW oscillator quickly responds to the on-off control of the power supply, which is suitable for intermittent operation. To verify the effectiveness of the circuit scheme, the authors fabricated a prototype sensor system. When the volume of urine is 0.2 ml, the battery outputs a voltage of over 1.3 V; and the sensor system can transmit signals over a distance of 5 m.

  14. Wearable flex sensor system for multiple badminton player grip identification

    Science.gov (United States)

    Jacob, Alvin; Zakaria, Wan Nurshazwani Wan; Tomari, Mohd Razali Bin Md; Sek, Tee Kian; Suberi, Anis Azwani Muhd

    2017-09-01

    This paper focuses on the development of a wearable sensor system to identify the different types of badminton grip that is used by a player during training. Badminton movements and strokes are fast and dynamic, where most of the involved movement are difficult to identify with the naked eye. Also, the usage of high processing optometric motion capture system is expensive and causes computational burden. Therefore, this paper suggests the development of a sensorized glove using flex sensor to measure a badminton player's finger flexion angle. The proposed Hand Monitoring Module (HMM) is connected to a personal computer through Bluetooth to enable wireless data transmission. The usability and feasibility of the HMM to identify different grip types were examined through a series of experiments, where the system exhibited 70% detection ability for the five different grip type. The outcome plays a major role in training players to use the proper grips for a badminton stroke to achieve a more powerful and accurate stroke execution.

  15. Elliptic Curve Cryptography with Security System in Wireless Sensor Networks

    Science.gov (United States)

    Huang, Xu; Sharma, Dharmendra

    2010-10-01

    The rapid progress of wireless communications and embedded micro-electro-system technologies has made wireless sensor networks (WSN) very popular and even become part of our daily life. WSNs design are generally application driven, namely a particular application's requirements will determine how the network behaves. However, the natures of WSN have attracted increasing attention in recent years due to its linear scalability, a small software footprint, low hardware implementation cost, low bandwidth requirement, and high device performance. It is noted that today's software applications are mainly characterized by their component-based structures which are usually heterogeneous and distributed, including the WSNs. But WSNs typically need to configure themselves automatically and support as hoc routing. Agent technology provides a method for handling increasing software complexity and supporting rapid and accurate decision making. This paper based on our previous works [1, 2], three contributions have made, namely (a) fuzzy controller for dynamic slide window size to improve the performance of running ECC (b) first presented a hidden generation point for protection from man-in-the middle attack and (c) we first investigates multi-agent applying for key exchange together. Security systems have been drawing great attentions as cryptographic algorithms have gained popularity due to the natures that make them suitable for use in constrained environment such as mobile sensor information applications, where computing resources and power availability are limited. Elliptic curve cryptography (ECC) is one of high potential candidates for WSNs, which requires less computational power, communication bandwidth, and memory in comparison with other cryptosystem. For saving pre-computing storages recently there is a trend for the sensor networks that the sensor group leaders rather than sensors communicate to the end database, which highlighted the needs to prevent from the man

  16. Thermodynamics of two component gaseous and solid state plasmas at any degeneracy

    International Nuclear Information System (INIS)

    Kraeft, W.D.; Stolzmann, W.; Fromhold-Treu, I.; Rother, T.

    1988-10-01

    We give the results of thermodynamical calculations for two component plasmas which are of interest for dense hydrogen, noble gas and alkali plasmas and for electron hole plasmas in optically excited semiconductors as well. 25 refs, 4 figs

  17. A novel design of an automatic lighting control system for a wireless sensor network with increased sensor lifetime and reduced sensor numbers.

    Science.gov (United States)

    Mohamaddoust, Reza; Haghighat, Abolfazl Toroghi; Sharif, Mohamad Javad Motahari; Capanni, Niccolo

    2011-01-01

    Wireless sensor networks (WSN) are currently being applied to energy conservation applications such as light control. We propose a design for such a system called a lighting automatic control system (LACS). The LACS system contains a centralized or distributed architecture determined by application requirements and space usage. The system optimizes the calculations and communications for lighting intensity, incorporates user illumination requirements according to their activities and performs adjustments based on external lighting effects in external sensor and external sensor-less architectures. Methods are proposed for reducing the number of sensors required and increasing the lifetime of those used, for considerably reduced energy consumption. Additionally we suggest methods for improving uniformity of illuminance distribution on a workplane's surface, which improves user satisfaction. Finally simulation results are presented to verify the effectiveness of our design.

  18. A Novel Design of an Automatic Lighting Control System for a Wireless Sensor Network with Increased Sensor Lifetime and Reduced Sensor Numbers

    Science.gov (United States)

    Mohamaddoust, Reza; Haghighat, Abolfazl Toroghi; Sharif, Mohamad Javad Motahari; Capanni, Niccolo

    2011-01-01

    Wireless sensor networks (WSN) are currently being applied to energy conservation applications such as light control. We propose a design for such a system called a Lighting Automatic Control System (LACS). The LACS system contains a centralized or distributed architecture determined by application requirements and space usage. The system optimizes the calculations and communications for lighting intensity, incorporates user illumination requirements according to their activities and performs adjustments based on external lighting effects in external sensor and external sensor-less architectures. Methods are proposed for reducing the number of sensors required and increasing the lifetime of those used, for considerably reduced energy consumption. Additionally we suggest methods for improving uniformity of illuminance distribution on a workplane’s surface, which improves user satisfaction. Finally simulation results are presented to verify the effectiveness of our design. PMID:22164114

  19. Nano-Composite Foam Sensor System in Football Helmets.

    Science.gov (United States)

    Merrell, A Jake; Christensen, William F; Seeley, Matthew K; Bowden, Anton E; Fullwood, David T

    2017-12-01

    American football has both the highest rate of concussion incidences as well as the highest number of concussions of all contact sports due to both the number of athletes and nature of the sport. Recent research has linked concussions with long term health complications such as chronic traumatic encephalopathy and early onset Alzheimer's. Understanding the mechanical characteristics of concussive impacts is critical to help protect athletes from these debilitating diseases and is now possible using helmet-based sensor systems. To date, real time on-field measurement of head impacts has been almost exclusively measured by devices that rely on accelerometers or gyroscopes attached to the player's helmet, or embedded in a mouth guard. These systems monitor motion of the head or helmet, but do not directly measure impact energy. This paper evaluates the accuracy of a novel, multifunctional foam-based sensor that replaces a portion of the helmet foam to measure impact. All modified helmets were tested using a National Operating Committee Standards for Athletic Equipment-style drop tower with a total of 24 drop tests (4 locations with 6 impact energies). The impacts were evaluated using a headform, instrumented with a tri-axial accelerometer, mounted to a Hybrid III neck assembly. The resultant accelerations were evaluated for both the peak acceleration and the severity indices. These data were then compared to the voltage response from multiple Nano Composite Foam sensors located throughout the helmet. The foam sensor system proved to be accurate in measuring both the HIC and Gadd severity index, as well as peak acceleration while also providing additional details that were previously difficult to obtain, such as impact energy.

  20. Stability equation and two-component Eigenmode for domain walls in scalar potential model

    International Nuclear Information System (INIS)

    Dias, G.S.; Graca, E.L.; Rodrigues, R. de Lima

    2002-08-01

    Supersymmetric quantum mechanics involving a two-component representation and two-component eigenfunctions is applied to obtain the stability equation associated to a potential model formulated in terms of two coupled real scalar fields. We investigate the question of stability by introducing an operator technique for the Bogomol'nyi-Prasad-Sommerfield (BPS) and non-BPS states on two domain walls in a scalar potential model with minimal N 1-supersymmetry. (author)

  1. Competitive Adsorption of a Two-Component Gas on a Deformable Adsorbent

    OpenAIRE

    Usenko, A. S.

    2013-01-01

    We investigate the competitive adsorption of a two-component gas on the surface of an adsorbent whose adsorption properties vary in adsorption due to the adsorbent deformation. The essential difference of adsorption isotherms for a deformable adsorbent both from the classical Langmuir adsorption isotherms of a two-component gas and from the adsorption isotherms of a one-component gas taking into account variations in adsorption properties of the adsorbent in adsorption is obtained. We establi...

  2. Three neural network based sensor systems for environmental monitoring

    International Nuclear Information System (INIS)

    Keller, P.E.; Kouzes, R.T.; Kangas, L.J.

    1994-05-01

    Compact, portable systems capable of quickly identifying contaminants in the field are of great importance when monitoring the environment. One of the missions of the Pacific Northwest Laboratory is to examine and develop new technologies for environmental restoration and waste management at the Hanford Site. In this paper, three prototype sensing systems are discussed. These prototypes are composed of sensing elements, data acquisition system, computer, and neural network implemented in software, and are capable of automatically identifying contaminants. The first system employs an array of tin-oxide gas sensors and is used to identify chemical vapors. The second system employs an array of optical sensors and is used to identify the composition of chemical dyes in liquids. The third system contains a portable gamma-ray spectrometer and is used to identify radioactive isotopes. In these systems, the neural network is used to identify the composition of the sensed contaminant. With a neural network, the intense computation takes place during the training process. Once the network is trained, operation consists of propagating the data through the network. Since the computation involved during operation consists of vector-matrix multiplication and application of look-up tables unknown samples can be rapidly identified in the field

  3. Determination of the number of and classification of two-component ionic-covalent chemical compounds

    International Nuclear Information System (INIS)

    Vigdorovich, V.N.; Dzhuraev, T.D.; Khanin, V.A.

    1989-01-01

    The aim of this work was to determine the number of and to classify two-component compounds corresponding to the four-electron and full-valence concepts and characterized by the ionic-covalent type of bond, on which the metallic bond is superimposed to a greater lesser degree. At the same time it was proposed to verify the position of the axes in the periodic system. The presence of numerous compound analogs for the element prototypes of one axis of the Mendeleev periodic system [the group of noble (inert) gases] was confirmed by computer experiments. However, the other axis (the carbon group) is not so obvious and is evidently due, on account of the superimposition of the effect of noncharacteristic (possible) valences, to the elements of various groups (boron, aluminum, germanium, antimony, bismuth). In addition, the compound analogs for the element prototypes of the d block are numerous, i.e., the copper-silver-gold, manganese-technetium-rhenium, and iron and platinum families

  4. Rayleigh-Taylor instability and mushroom-pattern formation in a two-component Bose-Einstein condensate

    International Nuclear Information System (INIS)

    Sasaki, Kazuki; Suzuki, Naoya; Saito, Hiroki; Akamatsu, Daisuke

    2009-01-01

    The Rayleigh-Taylor instability at the interface in an immiscible two-component Bose-Einstein condensate is investigated using the mean field and Bogoliubov theories. Rayleigh-Taylor fingers are found to grow from the interface and mushroom patterns are formed. Quantized vortex rings and vortex lines are then generated around the mushrooms. The Rayleigh-Taylor instability and mushroom-pattern formation can be observed in a trapped system.

  5. A Community-Based Event Delivery Protocol in Publish/Subscribe Systems for Delay Tolerant Sensor Networks

    Directory of Open Access Journals (Sweden)

    Haigang Gong

    2009-09-01

    Full Text Available The basic operation of a Delay Tolerant Sensor Network (DTSN is to finish pervasive data gathering in networks with intermittent connectivity, while the publish/subscribe (Pub/Sub for short paradigm is used to deliver events from a source to interested clients in an asynchronous way. Recently, extension of Pub/Sub systems in DTSNs has become a promising research topic. However, due to the unique frequent partitioning characteristic of DTSNs, extension of a Pub/Sub system in a DTSN is a considerably difficult and challenging problem, and there are no good solutions to this problem in published works. To ad apt Pub/Sub systems to DTSNs, we propose CED, a community-based event delivery protocol. In our design, event delivery is based on several unchanged communities, which are formed by sensor nodes in the network according to their connectivity. CED consists of two components: event delivery and queue management. In event delivery, events in a community are delivered to mobile subscribers once a subscriber comes into the community, for improving the data delivery ratio. The queue management employs both the event successful delivery time and the event survival time to decide whether an event should be delivered or dropped for minimizing the transmission overhead. The effectiveness of CED is demonstrated through comprehensive simulation studies.

  6. A community-based event delivery protocol in publish/subscribe systems for delay tolerant sensor networks.

    Science.gov (United States)

    Liu, Nianbo; Liu, Ming; Zhu, Jinqi; Gong, Haigang

    2009-01-01

    The basic operation of a Delay Tolerant Sensor Network (DTSN) is to finish pervasive data gathering in networks with intermittent connectivity, while the publish/subscribe (Pub/Sub for short) paradigm is used to deliver events from a source to interested clients in an asynchronous way. Recently, extension of Pub/Sub systems in DTSNs has become a promising research topic. However, due to the unique frequent partitioning characteristic of DTSNs, extension of a Pub/Sub system in a DTSN is a considerably difficult and challenging problem, and there are no good solutions to this problem in published works. To ad apt Pub/Sub systems to DTSNs, we propose CED, a community-based event delivery protocol. In our design, event delivery is based on several unchanged communities, which are formed by sensor nodes in the network according to their connectivity. CED consists of two components: event delivery and queue management. In event delivery, events in a community are delivered to mobile subscribers once a subscriber comes into the community, for improving the data delivery ratio. The queue management employs both the event successful delivery time and the event survival time to decide whether an event should be delivered or dropped for minimizing the transmission overhead. The effectiveness of CED is demonstrated through comprehensive simulation studies.

  7. Multi-Sensor Systems Development for UXO Detection and Discrimination: Hand-Held Dual Magnetic/Electromagnetic Induction Sensor

    National Research Council Canada - National Science Library

    Wright, David; Bennett, Jr., , Hollis H; Dove, Linda P; Butler, Dwain K

    2008-01-01

    ...) detection and discrimination system. This breakthrough technology markedly reduces UXO false alarm rates by fusing two heretofore incompatible sensor platforms, integrating highly accurate spatial data in real time, and applying...

  8. Los Alamos energetic particle sensor systems at geostationary orbit

    International Nuclear Information System (INIS)

    Baker, D.N.; Aiello, W.; Asbridge, J.R.; Belian, R.D.; Higbie, P.R.; Klebesadel, R.W.; Laros, J.G.; Tech, E.R.

    1985-01-01

    The Los Alamos National Laboratory has provided energetic particle sensors for a variety of spacecraft at the geostationary orbit (36,000 km altitude). The sensor system called the Charged Particle Analyzer (CPA) consists of four separate subsystems. The LoE and HiE subsystems measure electrons in the energy ranges 30 to 300 keV and 200 to 2000 keV, respectively. The LoP and HiP subsystems measure ions in the ranges 100 to 600 keV and 0.40 to 150 MeV, respectively. A separate sensor system called the spectrometer for energetic electrons (SEE) measures very high-energy electrons (2 to 15 MeV) using advanced scintillator design. In this paper we describe the relationship of operational anomalies and spacecraft upsets to the directly measured energetic particle environments at 6.6 R/sub E/. We also compare and contrast the CPA and SEE instrument design characteristics with the next generation of Los Alamos instruments to be flown at geostationary altitudes

  9. Remote Power Systems for Sensors on the Northern Border

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, Lin J [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Kandt, Alicen J [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-06-08

    The National Renewable Energy Laboratory (NREL) is working with the Department of Homeland Security (DHS) [1] to field sensors that accurately track different types of transportation across the northern border of the U.S.. To do this, the sensors require remote power so that they can be placed in the most advantageous geographical locations, often where no grid power is available. This enables the sensors to detect and track aircraft/vehicles despite natural features (e.g., mountains, ridges, valleys, trees) that often prevent standard methods (e.g., monostatic radar or visual observers) from detecting them. Without grid power, portable power systems were used to provide between 80 and 300 W continuously, even in bitter cold and when buried under feet of snow/ice. NREL provides details about the design, installation, and lessons learned from long-term deployment of a second-generation of novel power systems that used adjustable-angle photovoltaics (PV), lithium ion batteries, and fuel cells that provide power to achieve 100% up-time.

  10. Enhanced performance of a filter-sensor system.

    Science.gov (United States)

    Sasaki, Isao; Josowicz, Mira; Janata, Jirí; Glezer, Ari

    2006-06-01

    In this paper are addressed two important, but seemingly unrelated issues: long term performance of a gas sensing array and performance of an air purification unit. It is shown that when considered together, the system can be regarded as a "smart filter". The enhancement is achieved by periodic differential sampling and measurement of the "upstream" and "downstream" gases of a filter. The correctly functioning filter supplies the "zero gas" from the downstream for the continuous sensor baseline correction. A key element in this scheme is the synthetic jet that delivers well-defined pulses of the two gases. The deterioration of the performance of the "smart filter" can be diagnosed from the response pattern of the sensor. The approach has been demonstrated on removal/sensing of ammonia gas from air.

  11. Data analysis of inertial sensor for train positioning detection system

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seong Jin; Park, Sung Soo; Lee, Jae Ho; Kang, Dong Hoon [Korea Railroad Research Institute, Uiwang (Korea, Republic of)

    2015-02-15

    Train positioning detection information is fundamental for high-speed railroad inspection, making it possible to simultaneously determine the status and evaluate the integrity of railroad equipment. This paper presents the results of measurements and an analysis of an inertial measurement unit (IMU) used as a positioning detection sensors. Acceleration and angular rate measurements from the IMU were analyzed in the amplitude and frequency domains, with a discussion on vibration and train motions. Using these results and GPS information, the positioning detection of a Korean tilting train express was performed from Naju station to Illo station on the Honam-line. The results of a synchronized analysis of sensor measurements and train motion can help in the design of a train location detection system and improve the positioning detection performance.

  12. Toward a multipoint optical fibre sensor system for use in process water systems based on artificial neural network pattern recognition

    International Nuclear Information System (INIS)

    King, D; Lyons, W B; Flanagan, C; Lewis, E

    2005-01-01

    An optical fibre sensor capable of detecting various concentrations of ethanol in water supplies is reported. The sensor is based on a U-bend sensor configuration and is incorporated into a 170-metre length of silica cladding silica core optical fibre. The sensor is interrogated using Optical Time Domain Reflectometry (OTDR) and it is proposed to apply artificial neural network (ANN) pattern recognition techniques to the resulting OTDR signals to accurately classify the sensor test conditions. It is also proposed that additional U-bend configuration sensors will be added to the fibre measurement length, in order to implement a multipoint optical fibre sensor system

  13. Integrated cable vibration control system using wireless sensors

    Science.gov (United States)

    Jeong, Seunghoo; Cho, Soojin; Sim, Sung-Han

    2017-04-01

    As the number of long-span bridges is increasing worldwide, maintaining their structural integrity and safety become an important issue. Because the stay cable is a critical member in most long-span bridges and vulnerable to wind-induced vibrations, vibration mitigation has been of interest both in academia and practice. While active and semi-active control schemes are known to be quite effective in vibration reduction compared to the passive control, requirements for equipment including data acquisition, control devices, and power supply prevent a widespread adoption in real-world applications. This study develops an integrated system for vibration control of stay-cables using wireless sensors implementing a semi-active control. Arduino, a low-cost single board system, is employed with a MEMS digital accelerometer and a Zigbee wireless communication module to build the wireless sensor. The magneto-rheological (MR) damper is selected as a damping device, controlled by an optimal control algorithm implemented on the Arduino sensing system. The developed integrated system is tested in a laboratory environment using a cable to demonstrate the effectiveness of the proposed system on vibration reduction. The proposed system is shown to reduce the vibration of stay-cables with low operating power effectively.

  14. Development of Abnormality Detection System for Bathers using Ultrasonic Sensors

    Science.gov (United States)

    Ohnishi, Yosuke; Abe, Takehiko; Nambo, Hidetaka; Kimura, Haruhiko; Ogoshi, Yasuhiro

    This paper proposes an abnormality detection system for bather sitting in bathtub. Increasing number of in-bathtub drowning accidents in Japan draws attention. Behind this large number of bathing accidents, Japan's unique social and cultural background come surface. For majority of people in Japan, bathing serves purpose in deep warming up of body, relax and enjoyable time. Therefore it is the custom for the Japanese to soak in bathtub. However overexposure to hot water may cause dizziness or fainting, which is possible to cause in-bathtub drowning. For drowning prevention, the system detects bather's abnormal state using an ultrasonic sensor array. The array, which has many ultrasonic sensors, is installed on the ceiling of bathroom above bathtub. The abnormality detection system uses the following two methods: posture detection and behavior detection. The function of posture detection is to estimate the risk of drowning by monitoring bather's posture. Meanwhile, the function of behavior detection is to estimate the risk of drowning by monitoring bather's behavior. By using these methods, the system detects bathers' different state from normal. As a result of experiment with a subject in the bathtub, the system was possible to detect abnormal state using subject's posture and behavior. Therefore the system is useful for monitoring bather to prevent drowning in bathtub.

  15. Multi-robot system using low-cost infrared sensors

    Directory of Open Access Journals (Sweden)

    Anubhav Kakkar

    2013-03-01

    Full Text Available This paper presents a proposed set of the novel technique, methods, and algorithm for simultaneous path planning, area exploration, area retrieval, obstacle avoidance, object detection, and object retrieval   autonomously by a multi-robot system. The proposed methods and algorithms are built considering the use of low cost infrared sensors with the ultimate function of efficiently exploring the given unknown area and simultaneously identifying desired objects by analyzing the physical characteristics of several of the objects that come across during exploration. In this paper, we have explained the scenario by building a coordinative multi-robot system consisting of two autonomously operated robots equipped with low-cost and low-range infrared sensors to perform the assigned task by analyzing some of the sudden changes in their environment. Along with identifying and retrieving the desired object, the proposed methodology also provide an inclusive analysis of the area being explored. The novelties presented in the paper may significantly provide a cost-effective solution to the problem of area exploration and finding a known object in an unknown environment by demonstrating an innovative approach of using the infrared sensors instead of high cost long range sensors and cameras. Additionally, the methodology provides a speedy and uncomplicated method of traversing a complicated arena while performing all the necessary and inter-related tasks of avoiding the obstacles, analyzing the area as well as objects, and reconstructing the area using all these information collected and interpreted for an unknown environment. The methods and algorithms proposed are simulated over a complex arena to depict the operations and manually tested over a physical environment which provided 78% correct results with respect to various complex parameters set randomly.

  16. Wide-beam sensors for controlling dual-delay systems

    Science.gov (United States)

    Edwards, J. B.; Twemlow, J. K.

    1982-09-01

    A class of dual delay feedback systems of open loop transfer function G(s) = k exp(-Xs)/l - exp(-Ws) is shown to be unstable if ratio X/W is noninteger. By means of z-transform techniques it is shown that, by using a feedback transducer that senses over a substantial distance either side of its central axis, closed-loop stability may be restored. Such transducers, termed widebeam sensors, include transmission, backscatter and natural radiation types as well as electromechanical conveyor belt weighers. Designing transducers for very narrow beams may not be desirable from the overall system viewpoint.

  17. Smart sensors and systems innovations for medical, environmental, and IoT applications

    CERN Document Server

    Yasuura, Hiroto; Liu, Yongpan; Lin, Youn-Long

    2017-01-01

    This book describes the technology used for effective sensing of our physical world and intelligent processing techniques for sensed information, which are essential to the success of Internet of Things (IoT). The authors provide a multidisciplinary view of sensor technology from materials, process, circuits, and big data domains and showcase smart sensor systems in real applications including smart home, transportation, medical, environmental, agricultural, etc. Unlike earlier books on sensors, this book provides a “global” view on smart sensors covering abstraction levels from device, circuit, systems, and algorithms. Profiles active research on smart sensors based on CMOS microelectronics; Describes applications of sensors and sensor systems in cyber physical systems, the social information infrastructure in our modern world; Includes coverage of a variety of related information technologies supporting the application of sensors; Discusses the integration of computation, networking, actuation, database...

  18. Design factors of sensors for the optical tracking systems of solar concentrators

    International Nuclear Information System (INIS)

    Klychev, Sh. I.; Fazylov, A. K.; Orlov, S. A.; Burbo, A. V.

    2011-01-01

    Basic diagrams for the sensors of the optical tracking systems of solar concentrators are considered, the design factors that determine their accuracy are analyzed, a new sensor design is suggested, and its optimal parameters are determined. (authors)

  19. PASSIVE WIRELESS MULTI-SENSOR TEMPERATURE AND PRESSURE SENSING SYSTEM USING ACOUSTIC WAVE DEVICES, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal describes the development of passive surface acoustic wave (SAW) sensors and multi-sensor systems for NASA application to remote wireless sensing of...

  20. Sensor

    OpenAIRE

    Gleeson, Helen; Dierking, Ingo; Grieve, Bruce; Woodyatt, Christopher; Brimicombe, Paul

    2015-01-01

    An electrical temperature sensor (10) comprises a liquid crystalline material (12). First and second electrically conductive contacts (14), (16), having a spaced relationship there between, contact the liquid crystalline material (12). An electric property measuring device is electrically connected to the first and second contacts (14), (16) and is arranged to measure an electric property of the liquid crystalline material (12). The liquid crystalline material (12) has a transition temperatur...