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Sample records for tu boo kv

  1. Neutrino Interactions in MicroBooNE

    OpenAIRE

    Del Tutto, Marco

    2017-01-01

    MicroBooNE is a liquid-argon-based neutrino experiment, which began collecting data in Fermilab's Booster neutrino beam in October 2015. Physics goals of the experiment include probing the source of the anomalous excess of electron-like events in MiniBooNE. In addition to this, MicroBooNE is carrying out an extensive cross section physics program that will help to probe current theories on neutrino-nucleon interactions and nuclear effects. These proceedings summarise the status of MicroBooNE'...

  2. MiniBooNE Oscillation Results

    International Nuclear Information System (INIS)

    Djurcic, Zelimir

    2009-01-01

    These proceedings summarize the MiniBooNE ν μ → ν e results, describe the first (bar ν) μ → (bar ν) e result, and current analysis effort with the NuMI neutrinos detected in the miniBooNE detector

  3. TU-EF-204-03: Task-Based KV and MAs Optimization for Radiation Dose Reduction in CT: From FBP to Statistical Model-Based Iterative Reconstruction (MBIR)

    International Nuclear Information System (INIS)

    Gomez-Cardona, D; Li, K; Lubner, M G; Pickhardt, P J; Chen, G-H

    2015-01-01

    Purpose: The introduction of the highly nonlinear MBIR algorithm to clinical CT systems has made CNR an invalid metric for kV optimization. The purpose of this work was to develop a task-based framework to unify kV and mAs optimization for both FBP- and MBIR-based CT systems. Methods: The kV-mAs optimization was formulated as a constrained minimization problem: to select kV and mAs to minimize dose under the constraint of maintaining the detection performance as clinically prescribed. To experimentally solve this optimization problem, exhaustive measurements of detectability index (d’) for a hepatic lesion detection task were performed at 15 different mA levels and 4 kV levels using an anthropomorphic phantom. The measured d’ values were used to generate an iso-detectability map; similarly, dose levels recorded at different kV-mAs combinations were used to generate an iso-dose map. The iso-detectability map was overlaid on top of the iso-dose map so that for a prescribed detectability level d’, the optimal kV-mA can be determined from the crossing between the d’ contour and the dose contour that corresponds to the minimum dose. Results: Taking d’=16 as an example: the kV-mAs combinations on the measured iso-d’ line of MBIR are 80–150 (3.8), 100–140 (6.6), 120–150 (11.3), and 140–160 (17.2), where values in the parentheses are measured dose values. As a Result, the optimal kV was 80 and optimal mA was 150. In comparison, the optimal kV and mA for FBP were 100 and 500, which corresponded to a dose level of 24 mGy. Results of in vivo animal experiments were consistent with the phantom results. Conclusion: A new method to optimize kV and mAs selection has been developed. This method is applicable to both linear and nonlinear CT systems such as those using MBIR. Additional dose savings can be achieved by combining MBIR with this method. This work was partially supported by an NIH grant R01CA169331 and GE Healthcare. K. Li, D. Gomez-Cardona, M. G

  4. The MiniBooNE detector

    International Nuclear Information System (INIS)

    Aguilar-Arevalo, A.A.; Anderson, C.E.; Bartoszek, L.M.; Bazarko, A.O.; Brice, S.J.; Brown, B.C.; Bugel, L.; Cao, J.; Coney, L.; Conrad, J.M.; Cox, D.C.; Curioni, A.; Djurcic, Z.; Finley, D.A.; Fleming, B.T.; Ford, R.; Garcia, F.G.; Garvey, G.T.; Green, C.; Green, J.A.

    2009-01-01

    The MiniBooNE neutrino detector was designed and built to look for ν μ →ν e oscillations in the (sin 2 2θ,Δm 2 ) parameter space region where the LSND experiment reported a signal. The MiniBooNE experiment used a beam energy and baseline that were an order of magnitude larger than those of LSND so that the backgrounds and systematic errors would be completely different. This paper provides a detailed description of the design, function, and performance of the MiniBooNE detector.

  5. The MiniBooNE Detector

    OpenAIRE

    MiniBooNE Collaboration

    2008-01-01

    The MiniBooNE neutrino detector was designed and built to look for muon-neutrino to electron-neutrino oscillations in the mixing parameter space region where the LSND experiment reported a signal. The MiniBooNE experiment used a beam energy and baseline that were an order of magnitude larger than those of LSND so that the backgrounds and systematic errors would be completely different. This paper provides a detailed description of the design, function, and performance of the MiniBooNE detector.

  6. Physics Motivations of SciBooNE

    International Nuclear Information System (INIS)

    Hiraide, K.

    2007-01-01

    SciBooNE is a new experiment for measuring neutrino-nucleus cross sections around one GeV region, which is important for the interpretaion of neutrino oscillation experiments. Physics motivations of the experiment are described here

  7. Cross section analyses in MiniBooNE and SciBooNE experiments

    OpenAIRE

    Katori, Teppei

    2013-01-01

    The MiniBooNE experiment (2002-2012) and the SciBooNE experiment (2007-2008) are modern high statistics neutrino experiments, and they developed many new ideas in neutrino cross section analyses. In this note, I discuss selected topics of these analyses.

  8. MicroBooNE: The Search For The MiniBooNE Low Energy Excess

    Energy Technology Data Exchange (ETDEWEB)

    Kaleko, David [Columbia Univ., New York, NY (United States)

    2017-01-01

    This thesis describes work towards the search for a low energy excess in MicroBooNE. What MicroBooNE is, what the low energy excess is, and how one searches for the latter in the former will be described in detail.

  9. Current Status of the MiniBooNE Experiment

    OpenAIRE

    Ray, H.; collaboration, for the MiniBooNE

    2004-01-01

    MiniBooNE is an experiment designed to refute or confirm the LSND anti-nu_mu -> anti-nu_e oscillation result. MiniBooNE will look for oscillations of nu_mu -> nu_e in a closed-box appearance analysis. MiniBooNE began collecting data in 2002, and is expected to continue data taking through 2005. Current MiniBooNE results are presented.

  10. Sterile Neutrino Searches in MiniBooNE and MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Ignarra, Christina M. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2014-09-01

    Tension among recent short baseline neutrino experiments has pointed toward the possible need for the addition of one or more sterile (non-interacting) neutrino states into the existing neutrino oscillation framework. This thesis first presents the motivation for sterile neutrino models by describing the short-baseline anomalies that can be addressed with them. This is followed by a discussion of the phenomenology of these models. The MiniBooNE experiment and results are then described in detail, particularly the most recent antineutrino analysis. This will be followed by a discussion of global fits to world data, including the anomalous data sets. Lastly, future experiments will be addressed, especially focusing on the MicroBooNE experiment and light collection studies. In particular, understanding the degradation source of TPB, designing the TPB-coated plates for MicroBooNE and developing lightguide collection systems will be discussed. We find an excess of events in the MiniBooNE antineutrino mode results consistent with the LSND anomaly, but one that has a different energy dependence than the low-energy excess reported in neutrino mode. This disagreement creates tension within global fits which include up to three sterile neutrinos. The low-energy excess will be addressed by the MicroBooNE experiment, which is expected to start taking data in early 2015. Tension among existing experiments calls for additional, more decisive future experiments.

  11. Sterile Neutrino Searches in MiniBooNE and MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Ignarra, Christina M. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2014-09-01

    Tension among recent short baseline neutrino experiments has pointed toward the possible need for the addition of one or more sterile (non-interacting) neutrino states into the existing neutrino oscillation framework. This thesis fi rst presents the motivation for sterile neutrino models by describing the short-baseline anomalies that can be addressed with them. This is followed by a discussion of the phenomenology of these models. The MiniBooNE experiment and results are then described in detail, particularly the most recent antineutrino analysis. This will be followed by a discussion of global fits to world data, including the anomalous data sets. Lastly, future experiments will be addressed, especially focusing on the MicroBooNE experiment and light collection studies. In particular, understanding the degradation source of TPB, designing the TPB-coated plates for MicroBooNE and developing lightguide collection systems will be discussed. We find an excess of events in the MiniBooNE antineutrino mode results consistent with the LSND anomaly, but one that has a di fferent energy dependence than the low-energy excess reported in neutrino mode. This disagreement creates tension within global fi ts which include up to three sterile neutrinos. The low-energy excess will be addressed by the MicroBooNE experiment, which is expected to start taking data in early 2015. Tension among existing experiments calls for additional, more decisive future experiments.

  12. Prospects for Antineutrino Running at MiniBooNE

    OpenAIRE

    Wascko, M. O.

    2006-01-01

    MiniBooNE began running in antineutrino mode on 19 January, 2006. We describe the sensitivity of MiniBooNE to LSND-like nuebar oscillations and outline a program of antineutrino cross-section measurements necessary for the next generation of neutrino oscillation experiments. We describe three independent methods of constraining wrong-sign (neutrino) backgrounds in an antineutrino beam, and their application to the MiniBooNE antineutrino analyses.

  13. Tu nos aposte protege

    DEFF Research Database (Denmark)

    Frederiksen, Britta Olrik

    2007-01-01

    Aspirationen af p, t og k i dansk er stedmoderligt behandlet af den gren af den historiske sprogforskning der bygger på skriftligt kildemateriale. Skrivemåden tu nos aposte protege for tu nos ab hoste protege 'bekyt du os mod fjenden' i en tilskrift fra anden halvdel af 1300-tallet i...

  14. Maternal kisses are not effective in alleviating minor childhood injuries (boo-boos): a randomized, controlled and blinded study.

    Science.gov (United States)

    2015-12-01

    The practice of maternal kissing of minor injuries of childhood (boo-boos), though widely endorsed and practised, has never been demonstrated to be of benefit to children. To determine the efficacy, if any, of maternal kissing of boo-boos in toddlers. Randomized, controlled and double-blinded study of children with experimentally induced minor injuries. Control arms included both no intervention group and 'sham' (non-maternal) kissing. Children were blinded to the identity of the kisser in both the maternal and sham control groups. Outpatient research clinics in Ottawa, Canada. 943 maternal-toddler pairs recruited from the community. Toddler Discomfort Index (TDI) pre-injury, 1 and 5 minutes post-injury. One-minute and 5-minute TDI scores did not differ significantly between the maternal and sham kiss groups. Both of these groups had significantly higher TDI scores at 5 minutes compared to the no intervention group. Maternal kissing of boo-boos confers no benefit on children with minor traumatic injuries compared to both no intervention and sham kissing. In fact, children in the maternal kissing group were significantly more distressed at 5 minutes than were children in the no intervention group. The practice of maternal kissing of boo-boos is not supported by the evidence and we recommend a moratorium on the practice. © 2015 John Wiley & Sons, Ltd.

  15. The distribution of silicon on BP Boo

    International Nuclear Information System (INIS)

    Hatzes, A.P.

    1990-01-01

    A version of the Doppler imaging technique which incorporates the principles of maximum entropy reconstruction is used to derive the silicon distribution on the Ap star BP Boo (HR 5857). The method used made it possible to detect an error in the published photometric period and a new value of 1.29557 d was determined. The silicon distribution consists of two depleted spots of unequal area separated by about 180deg in longitude. These spots may coincide with the location of the magnetic poles of the star as in the case of γ 2 Ari. Near the larger of the depleted silicon spots is a spot of enhanced abundance. The unequal area of the depleted spots as well as the close proximity of the enhanced spot to one of the depleted regions suggests the presence of non-axisymmetric magnetic field lines. (author)

  16. The MicroBooNE Technical Design Report

    Energy Technology Data Exchange (ETDEWEB)

    Fleming, Bonnie [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2012-02-24

    MicroBooNE will build, operate, and extract physics from the first large liquid argon time projection chamber (LArTPC) that will be exposed to a high-intensity neutrino beam. With its unparalleled capabilities in tracking, vertexing, calorimetry, and particle identification, all with full electronic readout, MicroBooNE represents a major advance in detector technology for neutrino physics in the energy regime of most importance for elucidating oscillation phenomena.

  17. VizieR Online Data Catalog: Boo-127 and Boo-980 high-resolution spectra (Frebel+, 2016)

    Science.gov (United States)

    Frebel, A.; Norris, J. E.; Gilmore, G.; Wyse, R. F. G.

    2016-09-01

    We observed Boo-980 and Boo-127 stars with the MIKE spectrograph on the Magellan-Clay telescope in 2010 March and 2011 March. Details of the MIKE observations and photometry taken from Norris et al. (2008ApJ...689L.113N) are given in Table 1. MIKE spectra have nearly full optical wavelength coverage over the range ~3500-9000Å (R~22000 in the red and ~28000 in the blue wavelength regime). (2 data files).

  18. Leerstoel Installaties TU Delft

    NARCIS (Netherlands)

    Luscuere, P.G.; Zeiler, W.; Cauberg, J.J.M.; van der Linden, A.C.; Dobbelsteen, A.A.J.F.

    2007-01-01

    De leerstoel Installaties aan de TU Delft heeft een lange geschiedenis. De laatste decennia heeft Peter Luscuere de leerstoel in deeltijd bezet en zo een belangrijke bijdrage geleverd aan de koppeling van Onderzoek, Onderwijs en Ondernemen. Het artikel start met een korte beschrijving van de huidige

  19. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  20. Measurements of Neutrino Charged Current Interactions at SciBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Nakajima, Yasuhiro [Department of Physics, Kyoto University, Kyoto 606-8502 (Japan)], E-mail: nakajima@scphys.kyoto-u.ac.jp

    2009-08-15

    The SciBooNE experiment (FNAL-E954) is designed to measure neutrino-nucleous cross sections in the one GeV region. Additionally, SciBooNE serves as a near detector for MiniBooNE by measuring the neutrino flux. In this paper, we describe two analyses using neutrino charged current interactions at SciBooNE: a neutrino spectrum measurement and a search for charged current coherent pion production.

  1. Gaia's view of the λ Boo star puzzle

    Science.gov (United States)

    Murphy, Simon J.; Paunzen, Ernst

    2017-04-01

    The evolutionary status of the chemically peculiar class of λ Boo stars has been intensely debated. It is now agreed that the λ Boo phenomenon affects A stars of all ages, from star formation to the terminal age main sequence, but the cause of the chemical peculiarity is still a puzzle. We revisit the debate of their ages and temperatures in order to shed light on the phenomenon, using the new parallaxes in Gaia Data Release 1 with existing Hipparcos parallaxes and multicolour photometry. We find that no single formation mechanism is able to explain all the observations, and suggest that there are multiple channels producing λ Boo spectra. The relative importance of these channels varies with age, temperature and environment.

  2. KV7 potassium channels

    DEFF Research Database (Denmark)

    Stott, Jennifer B; Jepps, Thomas Andrew; Greenwood, Iain A

    2014-01-01

    Potassium channels are key regulators of smooth muscle tone, with increases in activity resulting in hyperpolarisation of the cell membrane, which acts to oppose vasoconstriction. Several potassium channels exist within smooth muscle, but the KV7 family of voltage-gated potassium channels have been...

  3. Core TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Etalle, Sandro

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We

  4. Power cables with extruded insulation and their accessories for rated voltages from 1 kV (Um = 1,2 kV) up to 30 kV (Um = 36 kV) : Part 2: cables for rated voltages from 6 kV (Um = 7,2 kV) up to 30 kV (Um = 36 kV)

    CERN Document Server

    International Electrotechnical Commission. Geneva

    2005-01-01

    Power cables with extruded insulation and their accessories for rated voltages from 1 kV (Um = 1,2 kV) up to 30 kV (Um = 36 kV) : Part 2: cables for rated voltages from 6 kV (Um = 7,2 kV) up to 30 kV (Um = 36 kV)

  5. Boo, a novel negative regulator of cell death, interacts with Apaf-1.

    OpenAIRE

    Song, Q; Kuang, Y; Dixit, V M; Vincenz, C

    1999-01-01

    In this report, we describe the cloning and characterization of Boo, a novel anti-apoptotic member of the Bcl-2 family. The expression of Boo was highly restricted to the ovary and epididymis implicating it in the control of ovarian atresia and sperm maturation. Boo contains the conserved BH1 and BH2 domains, but lacks the BH3 motif. Like Bcl-2, Boo possesses a hydrophobic C-terminus and localizes to intracellular membranes. Boo also has an N-terminal region with strong homology to the BH4 do...

  6. The SafeBoosC II randomized trial

    DEFF Research Database (Denmark)

    Plomgaard, Anne M; van Oeveren, Wim; Petersen, Tue Hvass

    2016-01-01

    BACKGROUND: The SafeBoosC phase II multicentre randomized clinical trial investigated the benefits and harms of monitoring cerebral oxygenation by near-infrared spectroscopy (NIRS) combined with an evidence-based treatment guideline vs. no NIRS data and treatment as usual in the control group...

  7. The SafeBoosC Phase II Randomised Clinical Trial

    DEFF Research Database (Denmark)

    Pellicer, Adelina; Greisen, Gorm; Benders, Manon

    2013-01-01

    Near-infrared spectroscopy-derived regional tissue oxygen saturation of haemoglobin (rStO2) reflects venous oxygen saturation. If cerebral metabolism is stable, rStO2 can be used as an estimate of cerebral oxygen delivery. The SafeBoosC phase II randomised clinical trial hypothesises that the bur...

  8. The SafeBoosC phase II clinical trial

    DEFF Research Database (Denmark)

    Riera, Joan; Hyttel-Sorensen, Simon; Bravo, María Carmen

    2016-01-01

    BACKGROUND: The SafeBoosC phase II randomised clinical trial recently demonstrated the benefits of a combination of cerebral regional tissue oxygen saturation (rStO2) by near-infrared spectroscopy (NIRS) and a treatment guideline to reduce the oxygen imbalance in extremely preterm infants. AIMS: ...

  9. MicroBooNE and its Cross Section Measurement

    Energy Technology Data Exchange (ETDEWEB)

    Tsai, Yun-Tse [SLAC

    2017-05-22

    MicroBooNE (the Micro Booster Neutrino Experiment) is a short-baseline neutrino experiment based on the technology of a liquid-argon time-projection chamber (LArTPC), and has recently completed its first year of data-taking in the Fermilab Booster Neutrino Beam. It aims to address the anomalous excess of events with an electromagnetic final state in MiniBooNE, to measure neutrino-argon interaction cross sections, and to provide relevant R\\&D for the future LArTPC experiments, such as DUNE. In these proceedings, we present the first reconstructed energy spectrum of Michel electrons from cosmic muon decays, the first kinematic distributions of the candidate muon tracks from $\

  10. Management of the complications of BPH/BOO

    Directory of Open Access Journals (Sweden)

    Mark J Speakman

    2014-01-01

    Full Text Available Most men will develop histological BPH if they live long enough. Approximately, half will develop benign prostatic enlargement (BPE and about half of these will get BOO with high bladder pressures and low flow, this in turn leads to detrusor wall hypertrophy. Many of these men will only have lower urinary tract symptoms (LUTS but a significant number will also suffer the other complications of BPH. These include urinary retention (acute and chronic, haematuria, urinary tract infection, bladder stones, bladder wall damage, renal dysfunction, incontinence and erectile dysfunction. Recognition of the complications of BPH/BOO early allows more effective management of these complications. This is particularly important for the more serious urinary infections and also for high-pressure chronic retention (HPCR. Complications of LUTS/BPH are very rare in clinical trials because of their strict inclusion and exclusion criteria but are more common in real life practice.

  11. Management of the complications of BPH/BOO.

    Science.gov (United States)

    Speakman, Mark J; Cheng, Xi

    2014-04-01

    Most men will develop histological BPH if they live long enough. Approximately, half will develop benign prostatic enlargement (BPE) and about half of these will get BOO with high bladder pressures and low flow, this in turn leads to detrusor wall hypertrophy. Many of these men will only have lower urinary tract symptoms (LUTS) but a significant number will also suffer the other complications of BPH. These include urinary retention (acute and chronic), haematuria, urinary tract infection, bladder stones, bladder wall damage, renal dysfunction, incontinence and erectile dysfunction. Recognition of the complications of BPH/BOO early allows more effective management of these complications. This is particularly important for the more serious urinary infections and also for high-pressure chronic retention (HPCR). Complications of LUTS/BPH are very rare in clinical trials because of their strict inclusion and exclusion criteria but are more common in real life practice.

  12. Toward Solution of the MiniBooNE-LSND Anomalies

    International Nuclear Information System (INIS)

    Karagiorgi, G.

    2012-01-01

    The LSND experiment has observed an anomalous excess of ν ¯ e events in a ν ¯ μ beam which can be interpreted as two-neutrino ν ¯ μ →ν ¯ e oscillations at Δm 2 ∼1 eV 2 , suggesting new physics. The MiniBooNE experiment has ruled out the LSND two-neutrino oscillation interpretation by looking for corresponding flavor oscillations in a neutrino beam, and assuming CP and CPT conservation. However, it has observed an anomalous excess of ν e events at low energy, which, to date, remains unexplained. The two excess signatures are discussed within the context of light sterile neutrino oscillations, and are further confronted with the latest results from a MiniBooNE search for ν ¯ μ →ν ¯ e oscillations in an antineutrino beam. Alternative interpretations are also referenced, along with possible future experimental tests.

  13. Bladder outlet obstruction (BOO) in female: etiology and management

    International Nuclear Information System (INIS)

    Shaikh, N.A.; Ahuja, K.; Shaikh, G.S.; Soomro, A.K.

    2015-01-01

    To determine the etiology and management outcome of bladder outlet obstruction (BOO) in female. Methodology: From 2009 to 2012, 37 females with a mean age of 40 (range 20-65) were investigated for etiology and management outcome of BOO. Typical complaints were slow urinary flow, difficulty in emptying bladder, frequency of micturition and urgency. Mean duration of symptoms was 6 month. Results: 15 women were confirmed as atrophic urethritis, 5 had functional bladder, 3 had urethral caruncle, 5 had cystocele, 7 had complete procedentia of uterus, and 2 had impacted urethral stone. Cystoscopy was performed in all patients to exclude other pathology like vesical stone and bladder growth. 12 patients were referred to Gynecology due to complete procedentia of uterus and cystocele. Three cases of urethral caruncle were treated by excision and biopsy, 2 patients with urethral stone were treated by endoscopic push back and litholapaxy while 5 required conservative treatment and 15 cases of atrophic urethritis were kept on Hormone Replacement Therapy (HRT). Conclusion: BOO is uncommon in female and management depends upon the etiology. (author)

  14. Neutrinos from the NuMI beamline in the MiniBooNE detector

    International Nuclear Information System (INIS)

    Aguilar-Arevalo, Alexis A.

    2006-01-01

    With the startup of the NuMI beamline early in 2005, the MiniBooNE detector has the unique opportunity to be the first user of an off-axis neutrino beam (110 mrad off-axis). MiniBooNE is assembling a rich sample of neutrino interactions from this source

  15. Low voltage 80 KV to 125 KV electron processors

    International Nuclear Information System (INIS)

    Lauppi, U.V.

    1999-01-01

    The classic electron beam technology made use of accelerating energies in the voltage range of 300 to 800 kV. The first EB processors - built for the curing of coatings - operated at 300 kV. The products to be treated were thicker than a simple layer of coating with thicknesses up to 100g and more. It was only in the beginning of the 1970's that industrial EB processors with accelerating voltages below 300 kV appeared on the market. Our company developed the first commercial electron accelerator without a beam scanner. The new EB machine featured a linear cathode, emitting a shower or 'curtain' of electrons over the full width of the product. These units were much smaller than anv previous EB processors and dedicated to the curing of coatings and other thin layers. ESI's first EB units operated with accelerating voltages between 150 and 200 kV. In 1993 ESI announced the introduction of a new generation of Electrocure. EB processors operating at 120 kV, and in 1998, at the RadTech North America '98 Conference in Chicago, the introduction of an 80 kV electron beam processor under the designation Microbeam LV

  16. Coronal Diagnostics of Intermediate Activity Star XI Boo A

    Science.gov (United States)

    Drake, Jeremy

    2005-01-01

    The analysis of Xi Boo A proved difficult to adapt to our line-by-line approach because of the strong wings of the RGS instrumental profile, as has been detailed in earlier reports. While progress was also delayed because of problems in using SAS v4, we succeeded in the past year or so to bring the analysis to conclusion. Abundances have been derived using both EPIC and RGS data, confirming earlier EUVE findings of a mild solar-like FIP effect, though with some evidence of a turn-up in abundances of elements with higher FIP. Plasma densities appear normal for a moderately active stellar corona. Xi Boo A nicely bridges the gap between the very active stars and stars like the Sun, and it indeed does appear that these are the stars in which the solar-like FIP effects begins to change to the "inverse FIP" type of effect seen in the very active stars. Probing this divide was the main goal of the proposal. These results are in the process of being prepared for publication, though we have not decided the target journal as yet.

  17. Searching for Exoplanet Effects on the X-ray Spectrum of τ Boo

    Science.gov (United States)

    Wood, Brian; Laming, J. Martin

    2018-01-01

    We study the X-ray spectrum of the exoplanet host star τ Boo A (F7 V), in order to explore the possibility that its very close-in, massive exoplanet (Porb=3.31 days, m sin i=3.9 MJ) may be affecting the coronal emissions of this star. The star was observed recently by Chandra/LETGS for 92 ksec in three pieces between 2017 February 27 and 2017 March 5; and was previously observed by XMM for 65 ksec in 2003 June 24. The new Chandra observations allow us to resolve τ Boo A from its stellar companion, τ Boo B (M2 V), for the first time. The companion accounts for 21% of the system's total X-ray emission at the time of the Chandra observation. Nevertheless, our measurements of τ Boo A emission measures and coronal abundances from Chandra are reasonably consistent with previous measurements from XMM by Maggio et al. (2011, A&A, 527, A144), in which τ Boo A and B are not resolved. Covering planetary orbital phases 0.21-0.31, 0.44-0.49, and 0.69-0.86, the Chandra data show that τ Boo A's coronal X-ray spectrum does not vary significantly with planetary orbital phase. However, our analysis suggests that coronal abundances for τ Boo A are somewhat anomalous, with a significantly weaker "FIP effect" compared to similar stars without close-in exoplanets, particularly π3 Ori (F6 V).

  18. Mechanosensitive gating of Kv channels.

    Directory of Open Access Journals (Sweden)

    Catherine E Morris

    Full Text Available K-selective voltage-gated channels (Kv are multi-conformation bilayer-embedded proteins whose mechanosensitive (MS Popen(V implies that at least one conformational transition requires the restructuring of the channel-bilayer interface. Unlike Morris and colleagues, who attributed MS-Kv responses to a cooperative V-dependent closed-closed expansion↔compaction transition near the open state, Mackinnon and colleagues invoke expansion during a V-independent closed↔open transition. With increasing membrane tension, they suggest, the closed↔open equilibrium constant, L, can increase >100-fold, thereby taking steady-state Popen from 0→1; "exquisite sensitivity to small…mechanical perturbations", they state, makes a Kv "as much a mechanosensitive…as…a voltage-dependent channel". Devised to explain successive gK(V curves in excised patches where tension spontaneously increased until lysis, their L-based model falters in part because of an overlooked IK feature; with recovery from slow inactivation factored in, their g(V datasets are fully explained by the earlier model (a MS V-dependent closed-closed transition, invariant L≥4. An L-based MS-Kv predicts neither known Kv time courses nor the distinctive MS responses of Kv-ILT. It predicts Kv densities (hence gating charge per V-sensor several-fold different from established values. If opening depended on elevated tension (L-based model, standard gK(V operation would be compromised by animal cells' membrane flaccidity. A MS V-dependent transition is, by contrast, unproblematic on all counts. Since these issues bear directly on recent findings that mechanically-modulated Kv channels subtly tune pain-related excitability in peripheral mechanoreceptor neurons we undertook excitability modeling (evoked action potentials. Kvs with MS V-dependent closed-closed transitions produce nuanced mechanically-modulated excitability whereas an L-based MS-Kv yields extreme, possibly excessive

  19. Orbital parameters of the multiple system EM Boo

    Science.gov (United States)

    Özkardeş, B.; Bakış, H.; Bakış, V.

    2018-02-01

    EM Boo is a relatively bright (V = 8.98 mag.) and short orbital period (P⁓2.45 days) binary star member of the multiple system WDS J14485+2445AB. There is neither photometric nor spectroscopic study of the system in the literature. In this work, we obtained spectroscopic orbital parameters of the system from new high resolution spectroscopic observations made with échelle spectrograph attached to UBT60 telescope of Akdeniz University. The spectroscopic solution yielded the values K1 = 100.7±2.6 km/s, K2 = 120.1±2.6 km/s and Vγ = -14.6±3.1 km/s, and thus the mass ratio of the system q = 0.838±0.064.

  20. Recent and relict topography of Boo Bee patch reef, Belize

    Science.gov (United States)

    Halley, R.B.; Shinn, E.A.; Hudson, J.H.; Lidz, B.; Taylor, D.L.

    1977-01-01

    Five core borings were taken on and around Boo Bee Patch Reef to better understand the origin of such shelf lagoon reefs. The cores reveal 4 stages of development: (1) subaerial exposure of a Pleistocene "high" having about 8 meters of relief, possibly a Pleistocene patch reef; (2) deposition of peat and impermeable terrigenous clay 3 meters thick around the high; (3) initiation of carbonate sediment production by corals and algae on the remaining 5 meters of hard Pleistocene topography and carbonate mud on the surrounding terrigenous clay; and (4) accelerated organic accumulation on the patch reef. Estimates of patch reef sedimentation rates (1.6 m/1000 years) are 3 to 4 times greater than off-reef sedimentation rates (0.4-0.5 m/1000 years). During periods of Pleistocene sedimentation on the Belize shelf, lagoon patch reefs may have grown above one another, stacking up to form reef accumulation of considerable thickness.

  1. TU electric reactor model verification

    International Nuclear Information System (INIS)

    Willingham, C.E.; Killgore, M.R.

    1989-01-01

    Power reactor benchmark calculations using the code package CASMO-3/SIMULATE-3 have been performed for six cycles of Prairie Island Unit 1. The reload fuel designs for the selected cycles include gadolinia as a burnable absorber, natural uranium axial blankets, and increased water-to-fuel ratio. The calculated results for both low-power physics tests (boron end points, control rod worths, and isothermal temperature coefficients) and full-power operation (power distributions and boron letdown) are compared to measured plant data. These comparisons show that the TU Electric reactor physics models accurately predict important physics parameters for power reactors

  2. Need for Different Cutoff Values for Reading Mantoux Test with 2TU and 5TU PPD.

    Science.gov (United States)

    Ramaraj, Savitha M; Nagendra, K; Gopal, Girish; Majgi, Sumanth Mallikarjuna

    2017-09-01

    To compare the tuberculin reaction of 2 tuberculin unit (TU) with 5TU purified protein derivative (PPD) (both calibrated against RT 23) in healthy children. This was a cross sectional study done in the pediatric outpatient department of a tertiary care teaching hospital. Seventy healthy siblings of the children attending pediatric outpatient department in the age group of 1 to 12 y were enrolled. The exclusion criteria included previously diagnosed tuberculosis patients, malnutrition diagnosed according to the WHO classification, history of drug intake like steroids, recent history of measles, any skin lesions over forearm, history of fever, contact with tuberculosis and previous mantoux testing. The study was conducted wherein each child was subjected to simultaneous testing with 2TU and 5TU by standard technique. The reactions to both the tests was read at 48-72 h. Children with induration ≥10 mm were evaluated for tuberculosis by taking chest x-ray, gastric lavage or sputum smear examination for acid fast bacilli (AFB). Considering ≥10 mm induration as positive, subjects positive with 5TU were 7 (10%) and 2TU was 1(p value = 0.031); thus, there is no agreement between the two methods (McNemar's test). Comparing the mean diameter of induration of 2TU and 5TU (p PPD is not comparable to that of 5TU PPD.

  3. Neutrino-nucleus neutral current elastic interactions measurement in MiniBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Perevalov, Denis [Univ. of Alabama, Tuscaloosa, AL (United States)

    2009-12-01

    The MiniBooNE experiment at the Fermi National Accelerator Laboratory (Fermilab) was designed to search for vμ → ve neutrino oscillations at Δm2 ~ 1 eV2 using an intense neutrino flux with an average energy Ev ~ 700 MeV. From 2002 to 2009 MiniBooNE has accumulated more than 1.0 x 1021 protons on target (POT) in both neutrino and antineutrino modes. MiniBooNE provides a perfect platform for detailed measurements of exclusive and semiinclusive neutrino cross-sections, for which MiniBooNE has the largest samples of events up to date, such as neutral current elastic (NCE), neutral current π0, charged current quasi-elastic (CCQE), charged current π+, and other channels. These measured cross-sections, in turn, allow to improve the knowledge of nucleon structure. This thesis is devoted to the study of NCE interactions. Neutrino-nucleus neutral current elastic scattering (vN → vN) accounts for about 18% of all neutrino interactions in MiniBooNE. Using a high-statistics, high purity sample of NCE interactions in MiniBooNE, the flux-averaged NCE differential cross-section has been measured and is being reported here. Further study of the NCE cross-section allowed for probing the structure of nuclei. The main interest in the NCE cross-section is that it may be sensitive to the strange quark contribution to the nucleon spin, Δs, this however requires a separation of NCE proton (vp → vp) from NCE neutron (vn → vn) events, which in general is a challenging task. MiniBooNE uses a Cherenkov detector, which imposes restrictions on the measured nucleon kinematic variables, mainly due to the impossibility to reconstruct the nucleon direction below the Cherenkov threshold. However, at kinetic energies above this threshold MiniBooNE is able to identify NCE proton events that do not experience final state interactions (FSI). These events were used for the Δs measurement. In this thesis

  4. Neutrino-nucleus neutral current elastic interactions measurement in MiniBooNE

    Science.gov (United States)

    Perevalov, Denis

    The MiniBooNE experiment at the Fermi National Accelerator Laboratory (Fermilab) was designed to search for numu → nu e neutrino oscillations at Deltam 2 ˜ 1 eV2 using an intense neutrino flux with an average energy Enu ˜ 700 MeV. From 2002 to 2009 MiniBooNE has accumulated more than 1.0x1021 protons on target (POT) in both neutrino and antineutrino modes. MiniBooNE provides a perfect platform for detailed measurements of exclusive and semi-inclusive neutrino cross-sections, for which MiniBooNE has the largest samples of events up to date, such as neutral current elastic (NCE), neutral current pi 0, charged current quasi-elastic (CCQE), charged current pi +, and other channels. These measured cross-sections, in turn, allow to improve the knowledge of nucleon structure. This thesis is devoted to the study of NCE interactions. Neutrino-nucleus neutral current elastic scattering (nuN → nu N) accounts for about 18% of all neutrino interactions in MiniBooNE. Using a high-statistics, high purity sample of NCE interactions in MiniBooNE, the flux-averaged NCE differential cross-section has been measured and is being reported here. Further study of the NCE cross-section allowed for probing the structure of nuclei. The main interest in the NCE cross-section is that it may be sensitive to the strange quark contribution to the nucleon spin, Deltas, this however requires a separation of NCE proton (nup → nu p) from NCE neutron (nun → nun ) events, which in general is a challenging task. MiniBooNE uses a Cherenkov detector, which imposes restrictions on the measured nucleon kinematic variables, mainly due to the impossibility to reconstruct the nucleon direction below the Cherenkov threshold. However, at kinetic energies above this threshold MiniBooNE is able to identify NCE proton events that do not experience final state interactions (FSI). These events were used for the Deltas measurement. In this thesis MiniBooNE reports the NCE (n+p) cross-section, the measurement

  5. The first orbital parameters and period variation of the short-period eclipsing binary AQ Boo

    Science.gov (United States)

    Wang, Shuai; Zhang, Liyun; Pi, Qingfeng; Han, Xianming L.; Zhang, Xiliang; Lu, Hongpeng; Wang, Daimei; Li, TongAn

    2016-10-01

    We obtained the first VRI CCD light curves of the short-period contact eclipsing binary AQ Boo, which was observed on March 22 and April 19 in 2014 at Xinglong station of National Astronomical Observatories, and on January 20, 21 and February 28 in 2015 at Kunming station of Yunnan Observatories of Chinese Academy of Sciences, China. Using our six newly obtained minima and the minima that other authors obtained previously, we revised the ephemeris of AQ Boo. By fitting the O-C (observed minus calculated) values of the minima, the orbital period of AQ Boo shows a decreasing tendency P˙ = - 1.47(0.17) ×10-7 days/year. We interpret the phenomenon by mass transfer from the secondary (more massive) component to the primary (less massive) one. By using the updated Wilson & Devinney program, we also derived the photometric orbital parameters of AQ Boo for the first time. We conclude that AQ Boo is a near contact binary with a low contact factor of 14.43%, and will become an over-contact system as the mass transfer continues.

  6. Non-invasive diagnosis of bladder outlet obstruction (BOO) in male patients with lower urinary tract symptoms (LUTS)

    NARCIS (Netherlands)

    Oelke, M.

    2016-01-01

    The thesis summarizes nine published articles and deals with the diagnostic value of morphological changes in the lower urinary tract in adult men with LUTS associated with BOO. Until recently, no morphological change of the lower urinary tract could safely diagnose BOO. Therefore, the value of

  7. Measurement of a Neutrino-Induced Charged Current Single Neutral Pion Cross Section at MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Hackenburg, Ariana [Yale U.

    2018-01-01

    Micro Booster Neutrino Experiment (MicroBooNE) is a Liquid Argon Time Projection Chamber (LArTPC) operating in the Booster Neutrino Beamline at Fermi National Accelerator Laboratory. MicroBooNE's physics goals include studying short basline $\

  8. Charged-Current Neutral Pion production at SciBooNE

    International Nuclear Information System (INIS)

    Catala-Perez, J.

    2009-01-01

    SciBooNE, located in the Booster Neutrino Beam at Fermilab, collected data from June 2007 to August 2008 to accurately measure muon neutrino and anti-neutrino cross sections on carbon below 1 GeV neutrino energy. SciBooNE is studying charged current interactions. Among them, neutral pion production interactions will be the focus of this poster. The experimental signature of neutrino-induced neutral pion production is constituted by two electromagnetic cascades initiated by the conversion of the π 0 decay photons, with an additional muon in the final state for CC processes. In this poster, I will present how we reconstruct and select charged-current muon neutrino interactions producing π 0 's in SciBooNE.

  9. Light equation in eclipsing binary CV Boo: third body candidate in elliptical orbit

    Science.gov (United States)

    Bogomazov, A. I.; Kozyreva, V. S.; Satovskii, B. L.; Krushevska, V. N.; Kuznyetsova, Y. G.; Ehgamberdiev, S. A.; Karimov, R. G.; Khalikova, A. V.; Ibrahimov, M. A.; Irsmambetova, T. R.; Tutukov, A. V.

    2016-12-01

    A short period eclipsing binary star CV Boo is tested for the possible existence of additional bodies in the system with a help of the light equation method. We use data on the moments of minima from the literature as well as from our observations during 2014 May-July. A variation of the CV Boo's orbital period is found with a period of {≈}75 d. This variation can be explained by the influence of a third star with a mass of {≈}0.4 M_{⊙} in an eccentric orbit with e≈0.9. A possibility that the orbital period changes on long time scales is discussed. The suggested tertiary companion is near the chaotic zone around the central binary, so CV Boo represents an interesting example to test its dynamical evolution. A list of 14 minima moments of the binary obtained from our observations is presented.

  10. Multi-band photometric study of the short-period eclipsing binary GR Boo

    Science.gov (United States)

    Wang, Daimei; Zhang, Liyun; Han, Xianming L.; Lu, Hongpeng

    2017-05-01

    We present BVRI light curves with complete phase coverage for the short-period (p = 0.377day) eclipsing binary star GR Boo. We carried out the observations using the SARA 90 cm telescope located at Kitt Peak National Observatory. We obtained six new light curve minimum times. By fitting all of the available O-C minimum times, we obtained an updated ephemeris that shows the orbital period of GR Boo is decreasing at a rate of P˙ = - 2.36 ×10-7 days/year. This decrease in its period can be explained by either mass transfer from the more massive component to the less massive one, or angular momentum exchange due to magnetic activities. We also obtained a set of revised orbital parameters using the Wilson & Devinney program. And finally, we concluded that GR Boo is a contact binary with a dark spot.

  11. Hvor fosser kvælstoffet hen?

    DEFF Research Database (Denmark)

    Larsen, Torben

    Der har i Ingeniøren inden i et par måneder kunne læses adskillelige ivrige indlæg om de danske fjordes evne til at fjerne kvælstof. Kvælstof fosser ud af fjordene? lød en af de første overskrifter. Få uger efter blev det stik modsatte synspunkt fremført under overskriften Fjordene holder på kvæl...

  12. Search for Sterile Neutrinos Using the MiniBooNE Beam

    Energy Technology Data Exchange (ETDEWEB)

    Sorel, Michel [Columbia Univ., New York, NY (United States)

    2005-01-01

    The possible existence of light sterile neutrinos in Nature is motivated, and the prospects to extend sterile neutrino searches beyond current limits is substantiated, using the MiniBooNE neutrino beam and detector at Fermilab. We report on the neutrino flux predictions for the MiniBooNE experiment, on the characterization of the charged-current, quasi-elastic interactions of muon neutrinos ({nu}{sub {mu}}n {yields} {mu}{sup -}p) observed, and on the experiment's sensitivity to sterile neutrinos via muon neutrino disappearance.

  13. The nucleon axial mass and the MiniBooNE quasielastic neutrino-nucleus scattering problem

    International Nuclear Information System (INIS)

    Nieves, J.; Ruiz Simo, I.; Vicente Vacas, M.J.

    2012-01-01

    The charged-current double differential neutrino cross section, measured by the MiniBooNE Collaboration, has been analyzed using a microscopical model that accounts for, among other nuclear effects, long range nuclear (RPA) correlations and multinucleon scattering. We find that MiniBooNE data are fully compatible with the world average of the nucleon axial mass in contrast with several previous analyses which have suggested an anomalously large value. We also discuss the reliability of the algorithm used to estimate the neutrino energy.

  14. Construction and assembly of the wire planes for the MicroBooNE Time Projection Chamber

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Adams, C.; Asaadi, J.; Danaher, J.; Fleming, B. T.; Gardner, R.; Gollapinni, S.; Grosso, R.; Guenette, R.; Littlejohn, B. R.; Lockwitz, S.; Raaf, J. L.; Soderberg, M.; John, J. St.; Strauss, T.; Szelc, A. M.; Yu, B.

    2017-03-01

    In this paper we describe how the readout planes for the MicroBooNE Time Projection Chamber were constructed, assembled and installed. We present the individual wire preparation using semi-automatic winding machines and the assembly of wire carrier boards. The details of the wire installation on the detector frame and the tensioning of the wires are given. A strict quality assurance plan ensured the integrity of the readout planes. The different tests performed at all stages of construction and installation provided crucial information to achieve the successful realization of the MicroBooNE wire planes.

  15. PHOTOMETRIC ANALYSIS OF HS Aqr, EG Cep, VW LMi, AND DU Boo

    Energy Technology Data Exchange (ETDEWEB)

    Djurasevic, G.; Latkovic, O. [Astronomical Observatory, Volgina 7, 11000 Belgrade (Serbia); Bastuerk, Oe.; Y Latin-Small-Letter-Dotless-I lmaz, M. [Astronomy and Space Sciences Research and Application Center, Ankara University, TR-06837 Ahlatl Latin-Small-Letter-Dotless-I bel, Ankara (Turkey); Cal Latin-Small-Letter-Dotless-I skan, S.; Senavc Latin-Small-Letter-Dotless-I , H. V.; K Latin-Small-Letter-Dotless-I l Latin-Small-Letter-Dotless-I coglu, T.; Ekmekci, F. [Department of Astronomy and Space Sciences, Faculty of Science, Ankara University, TR-06100 Tandogan, Ankara (Turkey); Tanr Latin-Small-Letter-Dotless-I verdi, T., E-mail: olivia@aob.rs [Department of Physics, Faculty of Arts and Sciences, Nigde University, 51240 Nigde (Turkey)

    2013-03-15

    We analyze new multicolor light curves for four close late-type binaries: HS Aqr, EG Cep, VW LMi, and DU Boo, in order to determine the orbital and physical parameters of the systems and estimate the distances. The analysis is done using the modeling code of G. Djurasevic, and is based on up-to-date measurements of spectroscopic elements. All four systems have complex, asymmetric light curves that we model by including bright or dark spots on one or both components. Our findings indicate that HS Aqr and EG Cep are in semi-detached, while VW LMi and DU Boo are in overcontact configurations.

  16. The electrically silent Kv6.4 subunit confers hyperpolarized gating charge movement in Kv2.1/Kv6.4 heterotetrameric channels.

    Directory of Open Access Journals (Sweden)

    Elke Bocksteins

    Full Text Available The voltage-gated K(+ (Kv channel subunit Kv6.4 does not form functional homotetrameric channels but co-assembles with Kv2.1 to form functional Kv2.1/Kv6.4 heterotetrameric channels. Compared to Kv2.1 homotetramers, Kv6.4 exerts a ~40 mV hyperpolarizing shift in the voltage-dependence of Kv2.1/Kv6.4 channel inactivation, without a significant effect on activation gating. However, the underlying mechanism of this Kv6.4-induced modulation of Kv2.1 channel inactivation, and whether the Kv6.4 subunit participates in the voltage-dependent gating of heterotetrameric channels is not well understood. Here we report distinct gating charge movement of Kv2.1/Kv6.4 heterotetrameric channels, compared to Kv2.1 homotetramers, as revealed by gating current recordings from mammalian cells expressing these channels. The gating charge movement of Kv2.1/Kv6.4 heterotetrameric channels displayed an extra component around the physiological K(+ equilibrium potential, characterized by a second sigmoidal relationship of the voltage-dependence of gating charge movement. This distinct gating charge displacement reflects movement of the Kv6.4 voltage-sensing domain and has a voltage-dependency that matches the hyperpolarizing shift in Kv2.1/Kv6.4 channel inactivation. These results provide a mechanistic basis for the modulation of Kv2.1 channel inactivation gating kinetics by silent Kv6.4 subunits.

  17. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  18. French 400 kV network

    Energy Technology Data Exchange (ETDEWEB)

    Pelissier, R

    1965-11-01

    A 400 kV transmission network has been constructed to carry hydroelectric power from the Alps and the Massif Central to Paris at peak hours and to carry power in the reverse direction in off-peak hours. A double circuit-ring at 400 kV encircling the Paris region is also nearing completion. Measures have to be taken to counter the very high short-circuit currents in such a network. A 730 kV network will eventually become necessary. The consequent multiplicity of transmission voltages will give rise to further problems. Collaboration with neighboring countries is envisaged. The problems of stability and synchronization posed by the new system are described and solutions suggested. The new circuit-breaking requirements are discussed, and details of tower design for 400 kV and 730 kV are given.

  19. Antineutrino Neutral Current Interactions in MiniBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Dharmapalan, Ranjan [Univ. of Alabama, Tuscaloosa, AL (United States)

    2012-01-01

    This dissertation reports the antineutrino-nucleus neutral current elastic scattering cross section on CH2 measured by the MiniBooNE experiment located in Batavia, IL. The data set consists of 60,605 events passing the selection cuts corresponding to 10.1×1020 POT, which represents the world’s largest sample of antineutrino neutral current elastic scattering events. The final sample is more than one order of magnitude lager that the previous antineutrino NCE scattering cross section measurement reported by the BNL E734 experiment. The measurement presented in this dissertation also spans a wider range in Q2, including the low-Q2 regime where the cross section rollover is clearly visible. A X2-based minimization was performed to determine the best value of the axial mass, MA and the Pauli blocking scaling function, that matches the antineutrino NCE scattering data. However, the best fit values of MA=1.29 GeV and K=1.026 still give a relatively poor X2, which suggests that the underlying nuclear model (based largely on the relativistic Fermi gas model) may not be an accurate representation for this particular interaction. Additionally, we present a measurement of the antineutrino/neutrino-nucleus NCE scattering cross section ratio. The neutrino mode NCE sample used in this study, corresponding to 6.4 × 1020 POT, is also the world’s largest sample (also by an order of magnitude). We have demonstrated that the ratio measurement is robust, as most of the correlated errors cancel, as expected. Furthermore, this ratio also proves to be rather insensitive to variations in the axial mass and the Pauli blocking parameter. This is the first time that this ratio has been experimentally reported. We believe this measurement will aid the theoretical physics community to test various model predictions of neutrino-nucleon/nucleus interactions.

  20. Variations of brightness and Ca 2 emission of the xi Boo AB and HD 1835 dWarf G stars

    Energy Technology Data Exchange (ETDEWEB)

    Chuganov, P.F.

    1983-01-01

    75 photoelectric UBV-observations of xi Boo AB were obtained during 45 nights in 1980. Real light variations of several thousandths of magnitude are revealed. Moreover, the variations observed in two nights were resembling flares. Both recent and published data on HD 1835 together with Wilson's observations of H and K Ca II lines in the spectra of xi Boo A, xi Boo B and HD 1835 are used for the search of periodicities. The periodicities are present in variations of light and Ca II -emission. Periodicities are more prominent in light variations (periods are 10 days for xi Boo AB and 7.66 days for HD 1835). In H and K Ca II lines the duration of cycles is different but the basic period is the same as in light variations. Therefore, the basic period (interpreted as the period of star roration) can be revealed only from a very long run of H and K Ca II observations (approximately 150). Light variations of xi Boo AB are probably due to the A-component since the basic period of Ca II-variations in xi Boo A is approximately the same (10 days). The basic period of Ca II-variations in xi Boo B is considerably larger.

  1. Variations of brightness and Ca 2 emission of the xi Boo AB and HD 1835 dWarf G stars

    International Nuclear Information System (INIS)

    Chuganov, P.F.

    1983-01-01

    75 photoelectric UBV-observations of xi Boo AB were obtained during 45 nights in 1980. Real light variations of several thousandths of magnitude are revealed. Moreover, the variations observed in two nights were resembling flares. Both recent and published data on HD 1835 together with Wilson's observations of H and K Ca II lines in the spectra of xi Boo A, xi Boo B and HD 1835 are used for the search of periodicities. The periodicities are present in variations of light and Ca II -emission. Periodicities are more prominent in light variations (periods are 10 days for xi Boo AB and 7.66 days for HD 1835). In H and K Ca II lines the dura-- tion of cycles is different but the basic period is the same as in light variations. Therefore, the basic period (interpreted as the period of star roration) can be revealed only from a very long run of H and K Ca II observations (approximately 150). Light variations of xi Boo AB are probably due to the A-component since the basic period of Ca II-variations in xi Boo A is approximately the same (10 days). The basic period of Ca II-variations in xi Boo B is considerably larger;

  2. Progress On Neutrino-Proton Neutral-Current Scattering In MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Pate, Stephen [New Mexico State U.

    2017-01-16

    The MicroBooNE Experiment at the Fermi National Accelerator Laboratory, an 89-ton active mass liquid argon time projection chamber, affords a unique opportunity to observe low-$Q^2$ neutral-current neutrino-proton scattering events. Neutral-current neutrino-proton scattering at $Q^2 < 1$ GeV$^2$ is dominated by the proton's axial form factor, which can be written as a combination of contributions from the up, down, and strange quarks: $G_A(Q^2) = \\frac{1}{2}[-G_A^u(Q^2)+G_A^d(Q^2)+G_A^s(Q^2)]$. The contribution from up and down quarks has been established in past charged-current measurements. The contribution from strange quarks at low $Q^2$ remains unmeasured; this is of great interest since the strange quark contribution to the proton spin can be determined from the low-$Q^2$ behavior: $\\Delta S = G_A^s(Q^2=0)$. MicroBooNE began operating in the Booster Neutrino Beam in October 2015. I will present the status in observing isolated proton tracks in the MicroBooNE detector as a signature for neutral-current neutrino-proton events. The sensitivity of the MicroBooNE experiment for measuring the strange quark contribution to the proton spin will be discussed.

  3. First results of the magnetic field measurements on the G0 IV η Boo

    Science.gov (United States)

    Butkovskaya, V. V.; Plachinda, S. I.; Baklanova, D.; Pankov, N. F.

    2018-01-01

    Search for a magnetic field on η Boo has been performed over 50 nights in 1999 — 2014. Statistically significant magnetic field has been detected over 5 out of 50 nights. The total range of the longitudinal magnetic field variations is from -15.1±6.4 G to 23.1±9.6 G.

  4. 9 Boo is a K-giant with high abundance of lithium

    International Nuclear Information System (INIS)

    Khyanni, L.

    1984-01-01

    An unusually strong lithium resonance lipe lambda 6707.8 was detected in the spectrum of the K-giant 9 Boo. The lithium abundance lg Nsub(Li)=2.5+-0.5 is estimated from a theoretical curve of growth calculated for a model atmosphere with Tsub(eff)=4000 K, lg g=2.0

  5. Brain injury in the international multicenter randomized SafeBoosC phase II feasibility trial

    DEFF Research Database (Denmark)

    Plomgaard, Anne M; Hagmann, Cornelia; Alderliesten, Thomas

    2016-01-01

    BACKGROUND: Abnormal cerebral perfusion during the first days of life in preterm infants is associated with higher grades of intraventricular hemorrhages and lower developmental score. In SafeBoosC II, we obtained a significant reduction of cerebral hypoxia by monitoring cerebral oxygenation in c...

  6. Bladder outlet obstruction (BOO) in men with castration-resistant prostate cancer.

    Science.gov (United States)

    Rom, Maximilian; Waldert, Matthias; Schatzl, Georg; Swietek, Natalia; Shariat, Shahrokh F; Klatte, Tobias

    2014-07-01

    To evaluate the frequency of bladder outlet obstruction (BOO) and detrusor overactivity (DO) in patients with castration-resistant prostate cancer (CRPC) and lower urinary tract symptoms (LUTS). Our prospective urodynamics database was queried. Inclusion criteria were CRPC and an International Prostate Symptom Score (IPSS) ≥ 20. Exclusion criteria were previous local therapy to the prostate gland, known urethral stricture disease, and a neurological component of LUTS. Twenty-one patients were identified. Urodynamic findings were analysed and compared with those of a matched cohort of 42 patients with benign prostatic enlargement (BPE). The median age of patients in the CRPC group was 74 years, and the median prostate-specific antigen (PSA) level at the time of the urodynamic study was 90 ng/mL. According to the BOO index, three patients (14%) were obstructed, three were equivocally obstructed (14%) and 15 were unobstructed. DO was seen in 12 patients (57%). Compared with the BPE group, patients with CRPC had lower cystometric bladder capacities (P = 0.003), were less likely to have BOO (14 vs 43%, P = 0.009) and more likely to have DO (57 vs 29%, P = 0.028). This study generates the hypothesis that only a minority of CRPC patients with LUTS have BOO, and that more than half of patients have DO. LUTS in CRPC may therefore be seldom attributable to BOO, but are, at least in part, related to DO and reduced cystometric capacity. A urodynamic investigation may be necessary before palliative transurethral resection of the prostate to select appropriate candidates. Larger prospective studies are needed to confirm our findings. © 2013 The Authors. BJU International © 2013 BJU International.

  7. Teaching Tu Fu on the Night Shift.

    Science.gov (United States)

    Brady, Philip

    1995-01-01

    Describes a teacher's unsuccessful attempt to introduce the poetry of Tu Fu, a wayward bureaucrat of the T'ang dynasty, to a class of part-time students. Uses his students' resistance to this poetry as an occasion to discuss the importance of personal responses to poetry, as opposed to "correct" academic responses. (TB)

  8. OBSERVATIONS OF X-RAY OSCILLATIONS IN ξ BOO: EVIDENCE OF A FAST-KINK MODE IN THE STELLAR LOOPS

    International Nuclear Information System (INIS)

    Pandey, J. C.; Srivastava, A. K.

    2009-01-01

    We report the observations of X-ray oscillations during the flare in a cool active star ξ Boo for the first time. ξ Boo was observed by EPIC/MOS of the XMM-Newton satellite. The X-ray light curve is investigated with wavelet and periodogram analyses. Both analyses clearly show oscillations of the period of ∼1019 s. We interpret these oscillations as a fundamental fast-kink mode of magnetoacoustic waves.

  9. Searches for New Physics at MiniBooNE: Sterile Neutrinos and Mixing Freedom

    Energy Technology Data Exchange (ETDEWEB)

    Karagiorgi, Georgia S. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2010-09-01

    The MiniBooNE experiment was designed to perform a search for vμ → ve oscillations in a region of Δm2 and sin2 2θ very different from that allowed by standard, three-neutrino oscillations, as determined by solar and atmospheric neutrino experiments. This search was motivated by the LSND experimental observation of an excess of $\\bar{v}$e events in a $\\bar{v}$μ beam which was found compatible with two-neutrino oscillations at Δm2 ~ 1 eV2 and sin2 2{theta} < 1%. If confirmed, such oscillation signature could be attributed to the existence of a light, mostly-sterile neutrino, containing small admixtures of weak neutrino eigenstates. In addition to a search for vμ → ve oscillations, MiniBooNE has also performed a search for vμ → ve oscillations, which provides a test of the LSND two-neutrino oscillation interpretation that is independent of CP or CPT violation assumptions. This dissertation presents the MiniBooNE vμ → ve and vμ → ve analyses and results, with emphasis on the latter. While the neutrino search excludes the two-neutrino oscillation interpretation of LSND at 98% C.L., the antineutrino search shows an excess of events which is in agreement with the two-neutrino vμ → ve oscillation interpretation of LSND, and excludes the no oscillations hypothesis at 96% C.L. Even though the neutrino and antineutrino oscillation results from MiniBooNE disagree under the single sterile neutrino oscillation hypothesis, a simple extension to the model to include additional sterile neutrino states and the possibility of CP violation allows for differences between neutrino and antineutrino oscillation signatures. In view of that, the viability of oscillation models with one or two sterile neutrinos is investigated in global fits to MiniBooNE and LSND

  10. Decreased expression of Kv7 channels in Hirchsprung's disease.

    Science.gov (United States)

    O'Donnell, Anne-Marie; Coyle, David; Puri, Prem

    2017-07-01

    Voltage-dependent K + channels (Kv channels) participate in electrical rhythmicity and smooth muscle responses and are regulated by excitatory and inhibitory neurotransmitters. Kv channels also participate in the interstitial cell of Cajal (ICC) and smooth muscle cell (SMC) responses to neural inputs. The Kv family consists of 12 subfamilies, Kv1-Kv12, with five members of the Kv7 family identified to date: Kv7.1-Kv7.5. A recent study identified the potassium channel Kv7.5 as having a role in the excitability of ICC-IM in the mouse colon. We therefore designed this study to test the hypothesis that Kv7 channels are present in the normal human colon and are reduced in Hirschprung's disease (HSCR). HSCR tissue specimens were collected at the time of pull-through surgery (n=10), while normal control tissue specimens were obtained at the time of colostomy closure in patients with imperforate anus (n=10). Kv7.3-Kv7.5 immunohistochemistry was performed and visualized using confocal microscopy to assess their distribution. Western blot analysis was undertaken to determine Kv7.3-Kv7.5 protein quantification. Kv7.3 and Kv7.4-immunoreactivity was co-localized with neuron and ICC markers, while Kv7.5 was found to be expressed on both ICCs and SMCs. Western blot analysis revealed similar levels of Kv7.3 and Kv7.5 expression in the normal colon and HSCR colon, while Kv7.4 proteins were found to be markedly decreased in ganglionic specimens and decreased further in aganglionic specimens. A deficiency of Kv7.4 channels in the ganglionic and aganglionic bowel may place a role in colonic dysmotility in HSCR. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Electron Attenuation Measurement using Cosmic Ray Muons at the MicroBooNE LArTPC

    Energy Technology Data Exchange (ETDEWEB)

    Meddage, Varuna [Kansas State U., Manhattan

    2017-10-01

    The MicroBooNE experiment at Fermilab uses liquid argon time projection chamber (LArTPC) technology to study neutrino interactions in argon. A fundamental requirement for LArTPCs is to achieve and maintain a low level of electronegative contaminants in the liquid to minimize the capture of drifting ionization electrons. The attenuation time for the drifting electrons should be long compared to the maximum drift time, so that the signals from particle tracks that generate ionization electrons with long drift paths can be detected efficiently. In this talk we present MicroBooNE measurement of electron attenuation using cosmic ray muons. The result yields a minimum electron 1/e lifetime of 18 ms under typical operating conditions, which is long compared to the maximum drift time of 2.3 ms.

  12. The nucleon axial mass and the MiniBooNE CCQE neutrino-nucleus data

    International Nuclear Information System (INIS)

    Nieves, J; Ruiz Simo, I; Vacas, M J Vicente

    2013-01-01

    We analyze the MiniBooNE CCQE dσ/dT μ d cos θ μ data using a theoretical model that has proved to be quite successful in the analysis of nuclear reactions with electron, photon and pion probes. We find that RPA and multinucleon knockout turn out to be essential for the description of the MiniBooNE data. We show these measurements are fully compatible with former determinations of nucleon axial mass M A , in contrast with several previous analyses, which have suggested an anomalously large value. We find, M A = 1.08 ± 0.03 GeV. We also argue that the procedure, commonly used to reconstruct the neutrino energy for QE events from the muon angle and energy, could be unreliable for a wide region of the phase space, due to the large importance of multinucleon events.

  13. A combined muon-neutrino and electron-neutrino oscillation search at MiniBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Monroe, Jocelyn Rebecca [Columbia Univ., New York, NY (United States)

    2006-01-01

    MiniBooNE seeks to corroborate or refute the unconfirmed oscillation result from the LSND experiment. If correct, the result implies that a new kind of massive neutrino, with no weak interactions, participates in neutrino oscillations. MiniBooNE searches for vμ → ve oscillations with the Fermi National Accelerator Laboratory 8 GeV beam line, which produces a vμ beam with an average energy of ~ 0.8 GeV and an intrinsic ve content of 0.4%. The neutrino detector is a 6.1 m radius sphere filled with CH2, viewed by 1540 photo-multiplier tubes, and located 541 m downstream from the source. This work focuses on the estimation of systematic errors associated with the neutrino flux and neutrino interaction cross section predictions, and in particular, on constraining these uncertainties using in-situ MiniBooNE vμ charged current quasielastic (CCQE) scattering data. A data set with ~ 100,000 events is identified, with 91% CCQE purity. This data set is used to measure several parameters of the CCQE cross section: the axial mass, the Fermi momentum, the binding energy, and the functional dependence of the axial form factor on four-momentum transfer squared. Constraints on the vμ and ve fluxes are derived using the vμ CCQE data set. A Monte Carlo study of a combined vμ disappearance and ve appearance oscillation fit is presented, which improves the vμ → ve oscillation sensitivity of MiniBooNE with respect to a ve appearance-only fit by 1.2-1.5σ, depending on the value of Δm2.

  14. Far-Infrared and Millimeter Continuum Studies of K-Giants: Alpha Boo and Alpha Tau

    Science.gov (United States)

    Cohen, Martin; Carbon, Duane F.; Welch, William J.; Lim, Tanya; Forster, James R.; Goorvitch, David; Thigpen, William (Technical Monitor)

    2002-01-01

    We have imaged two normal, non-coronal, infrared-bright K-giants, alpha Boo and alpha Tau, in the 1.4-millimeter and 2.8-millimeter continuum using BIMA. These stars have been used as important absolute calibrators for several infrared satellites. Our goals are: (1) to probe the structure of their upper photospheres; (2) to establish whether these stars radiate as simple photospheres or possess long-wavelength chromospheres; and (3) to make a connection between millimeter-wave and far-infrared absolute flux calibrations. To accomplish these goals we also present ISO Long Wavelength Spectrometer (LWS) measurements of both these K-giants. The far-infrared and millimeter continuum radiation is produced in the vicinity of the temperature minimum in a Boo and a Tau, offering a direct test of the model photospheres and chromospheres for these two cool giants. We find that current photospheric models predict fluxes in reasonable agreement with those observed for those wavelengths which sample the upper photosphere, namely less than or equal to 170 micrometers in alpha Tau and less than or equal to 125 micrometers in alpha Boo. It is possible that alpha Tau is still radiative as far as 0.9 - 1.4 millimeters. We detect chromospheric radiation from both stars by 2.8 millimeters (by 1.4 millimeters in alpha Boo), and are able to establish useful bounds on the location of the temperature minimum. An attempt to interpret the chromospheric fluxes using the two-component "bifurcation model" proposed by Wiedemann et al. (1994) appears to lead to a significant contradiction.

  15. Magnetic Field Measurements of the Spotted Yellow Dwarf DE Boo During 2001-2004

    Science.gov (United States)

    Plachinda, S.; Baklanova, D.; Butkovskaya, V.; Pankov, N.

    2017-06-01

    Spectropolarimetric observations of DE Boo have been performed at Crimean astrophysical observatory during 18 nights in 2001-2004. We present the result of the longitudinal magnetic field measurements on this star. The magnetic field varies from +44 G to -36 G with mean Standard Error (SE) of 8.2 G. For full array of the magnetic field measurements the difference between experimental errors and Monte Carlo errors is not statistically significant.

  16. VizieR Online Data Catalog: BVRI light curves of GR Boo (Wang+, 2017)

    Science.gov (United States)

    Wang, D.; Zhang, L.; Han, X. L.; Lu, H.

    2017-11-01

    We observed the eclipsing binary GR Boo on May 12, 22 and 24 in 2015 using the SARA 90-cm telescope located at Kitt Peak National Observatory, Arizona, USA. This telescope was equipped with an ARC CCD camera with a resolution of 2048x2048pixels but used at 2x2 binning, resulting in 1024x1024pixels. We used the Bessel BVRI filters. (1 data file).

  17. Functional analysis of Kv1.2 and paddle chimera Kv channels in planar lipid bilayers

    Science.gov (United States)

    Tao, Xiao; MacKinnon, Roderick

    2010-01-01

    Summary Voltage-dependent K+ channels play key roles in shaping electrical signaling in both excitable as well as non-excitable cells. These channels open and close in response to the voltage changes across the cell membrane. Many studies have been carried out in order to understand the voltage sensing mechanism. Our laboratory recently determined the atomic structures of a mammalian voltage-dependent K+ channel Kv1.2 and a mutant of Kv1.2 named the ‘paddle-chimera’ channel, in which the voltage sensor paddle was transferred from Kv2.1 to Kv1.2. These two structures provide atomic descriptions of voltage-dependent channels with unprecedented clarity. Until now the functional integrity of these two channels biosynthesized in yeast cells have not been assessed. Here we report the electrophysiological and pharmacological properties of Kv1.2 and the paddle chimera channels in planar lipid bilayers. We demonstrate that Pichia yeast produce ‘normally functioning’ mammalian voltage-dependent K+ channels with qualitatively similar features to the Shaker K+ channel in the absence of the N-terminal inactivation gate, and that the paddle chimera mutant channel functions as well as Kv1.2. We find, however, that in several respects the Kv1.2 channel exhibits functional properties that are distinct from Kv1.2 channels reported in the literature. PMID:18638484

  18. Dark Matter Search in a Proton Beam Dump with MiniBooNE.

    Science.gov (United States)

    Aguilar-Arevalo, A A; Backfish, M; Bashyal, A; Batell, B; Brown, B C; Carr, R; Chatterjee, A; Cooper, R L; deNiverville, P; Dharmapalan, R; Djurcic, Z; Ford, R; Garcia, F G; Garvey, G T; Grange, J; Green, J A; Huelsnitz, W; de Icaza Astiz, I L; Karagiorgi, G; Katori, T; Ketchum, W; Kobilarcik, T; Liu, Q; Louis, W C; Marsh, W; Moore, C D; Mills, G B; Mirabal, J; Nienaber, P; Pavlovic, Z; Perevalov, D; Ray, H; Roe, B P; Shaevitz, M H; Shahsavarani, S; Stancu, I; Tayloe, R; Taylor, C; Thornton, R T; Van de Water, R; Wester, W; White, D H; Yu, J

    2017-06-02

    The MiniBooNE-DM Collaboration searched for vector-boson mediated production of dark matter using the Fermilab 8-GeV Booster proton beam in a dedicated run with 1.86×10^{20} protons delivered to a steel beam dump. The MiniBooNE detector, 490 m downstream, is sensitive to dark matter via elastic scattering with nucleons in the detector mineral oil. Analysis methods developed for previous MiniBooNE scattering results were employed, and several constraining data sets were simultaneously analyzed to minimize systematic errors from neutrino flux and interaction rates. No excess of events over background was observed, leading to a 90% confidence limit on the dark matter cross section parameter, Y=ε^{2}α_{D}(m_{χ}/m_{V})^{4}≲10^{-8}, for α_{D}=0.5 and for dark matter masses of 0.01dark matter. This is the best limit from a dedicated proton beam dump search in this mass and coupling range and extends below the mass range of direct dark matter searches. These results demonstrate a novel and powerful approach to dark matter searches with beam dump experiments.

  19. VizieR Online Data Catalog: First analysis of the binary IK Boo (Kriwattanawong+, 2017)

    Science.gov (United States)

    Kriwattanawong, W.; Sanguansak, N.; Maungkorn, S.

    2017-03-01

    With new CCD observations of the W UMa type binary IK Boo, we present the first investigation of photometric parameters and orbital period change. The BVRc light curve fit shows that IK Boo is a W-type contact system with a mass ratio of q=1.146 and a shallow contact with a fill-out factor of f=2.22%. The orbital period decrease is found at a rate of -3.28x10-7d/yr, corresponding to a mass transfer from the more massive to the less massive component with a rate of -2.83x10-6M_ȯ/yr. The inner and outer critical Roche lobes will contract and cause the contact degree to increase. Therefore, IK Boo may evolve into a deeper contact system. Furthermore, a possible cyclic variation is found to have a period of 9.74yr, that could be explained by the light-travel time effect, due to the existence of a third companion in the system. (2 data files).

  20. Tests of Lorentz and CPT violation with MiniBooNE neutrino oscillation excesses

    International Nuclear Information System (INIS)

    Katori, Teppei

    2014-01-01

    Lorentz and CPT symmetry violaton is a predicted phenomenon of Planck–scale physics. Various types of data are analyzed to search for Lorentz violation under the Standard–Model Extension (SME) framework, including neutrino oscillation data. MiniBooNE is a short–baseline neutrino oscillation experiment at Fermilab. The measured excesses from MiniBooNE cannot be reconciled within the neutrino Standard Model (vSM); thus it might be a signal of new physics, such as Lorentz violation. We have analyzed the sidereal time dependence of MiniBooNE data for signals of the possible sidereal time dependence of the ocillation signals. we find that the v e appearance data prefer a sidereal time–independent solution, and the v-bar e appearance data slightly prefer a sidereal time–dependent solution, however, the statistical significance is not high to claim the discovery. Limits of order 10 −20 GeV are placed on combinations of SME coefficients

  1. Noise Characterization and Filtering in the MicroBooNE Liquid Argon TPC

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; et al.

    2017-05-20

    The low-noise operation of readout electronics in a liquid argon time projection chamber (LArTPC) is critical to properly extract the distribution of ionization charge deposited on the wire planes of the TPC, especially for the induction planes. This paper describes the characteristics and mitigation of the observed noise in the MicroBooNE detector. The MicroBooNE's single-phase LArTPC comprises two induction planes and one collection sense wire plane with a total of 8256 wires. Current induced on each TPC wire is amplified and shaped by custom low-power, low-noise ASICs immersed in the liquid argon. The digitization of the signal waveform occurs outside the cryostat. Using data from the first year of MicroBooNE operations, several excess noise sources in the TPC were identified and mitigated. The residual equivalent noise charge (ENC) after noise filtering varies with wire length and is found to be below 400 electrons for the longest wires (4.7 m). The response is consistent with the cold electronics design expectations and is found to be stable with time and uniform over the functioning channels. This noise level is significantly lower than previous experiments utilizing warm front-end electronics.

  2. Core TuLiP - Logic Programming for Trust Management

    NARCIS (Netherlands)

    Czenko, M.R.; Etalle, Sandro; Dahl, V.; Niemelä, I.

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We

  3. Early biomarkers of brain injury and cerebral hypo- and hyperoxia in the SafeBoosC II trial

    DEFF Research Database (Denmark)

    Plomgaard, Anne M.; Alderliesten, Thomas; Austin, Topun

    2017-01-01

    Background The randomized clinical trial, SafeBoosC II, examined the effect of monitoring of cerebral oxygenation by near-infrared spectroscopy combined with a guideline on treatment when cerebral oxygenation was out of the target range. Data on cerebral oxygenation was collected in both the inte......Background The randomized clinical trial, SafeBoosC II, examined the effect of monitoring of cerebral oxygenation by near-infrared spectroscopy combined with a guideline on treatment when cerebral oxygenation was out of the target range. Data on cerebral oxygenation was collected in both......, and the biomarkers of brain injury from birth till term equivalent age that was collected as secondary and explorative outcomes in the SafeBoosC II trial. Methods Cerebral oxygenation was continuously monitored during the first 72h of life in 166 extremely preterm infants. Cranial ultrasound was performed at day 1...

  4. 250 kV aim for hvdc valves

    Energy Technology Data Exchange (ETDEWEB)

    1966-06-01

    Development now being carried out by ASEA is aimed at increasing the normal operating voltage for a mercury arc valve to 250 kV dc. The maximum direct voltage per valve group, with one valve in each arm of the bridge, is 125 kV for equipment already in operation in New Zealand, Japan, and Konti Scan. Valves for 130 kV and 133 kV operation are under construction for the Vancouver and the Pacific Intertie 1 links.

  5. Functional properties of human neuronal Kv11 channels

    DEFF Research Database (Denmark)

    Einarsen, Karoline; Calloe, Kirstine; Grunnet, Morten

    2009-01-01

    Kv11 potassium channels are important for regulation of the membrane potential. Kv11.2 and Kv11.3 are primarily found in the nervous system, where they most likely are involved in the regulation of neuronal excitability. Two isoforms of human Kv11.2 have been published so far. Here, we present...... current characteristics of the isoforms presented in this work may contribute to the regulation of neuronal excitability....

  6. MiniBooNE Neutrino Physics at the University of Alabama

    International Nuclear Information System (INIS)

    Stancu, Ion

    2007-01-01

    This report summarizes the activities conducted by the UA group under the auspices of the DoE/EPSCoR grant number DE--FG02--04ER46112 since the date of the previous progress report, i.e., since November 2005. It also provides a final report of the accomplishments achieved during the entire period of this grant (February 2004 to January 2007). The grant has fully supported the work of Dr. Yong Liu (postdoctoral research assistant -- in residence at Fermilab) on the MiniBooNE reconstruction and particle identification (PID) algorithms.

  7. LSND versus MiniBooNE: Sterile neutrinos with energy dependent masses and mixing?

    CERN Document Server

    Schwetz, Thomas

    2008-01-01

    Standard active-sterile neutrino oscillations do not provide a satisfactory description of the LSND evidence for neutrino oscillations together with the constraints from MiniBooNE and other null-result short-baseline oscillation experiments. However, if the mass or the mixing of the sterile neutrino depends in an exotic way on its energy all data become consistent. I explore the phenomenological consequences of the assumption that either the mass or the mixing scales with the neutrino energy as $1/E_\

  8. Chemical composition of δ Scuti stars: 1. AO CVn, CP Boo, KW Aur

    Science.gov (United States)

    Galeev, A. I.; Ivanova, D. V.; Shimansky, V. V.; Bikmaev, I. F.

    2012-11-01

    We used high-resolution echelle spectra acquired with the 1.5-m Russian-Turkish Telescope to determine the fundamental atmospheric parameters and abundances of 30 chemical elements for three δ Scuti stars: AOCVn, CP Boo, and KWAur. The chemical compositions we find for these stars are similar to those for Am-star atmospheres, though some anomalies of up to 0.6-0.7 dex are observed for light and heavy elements. We consider the effect of the adopted stellar parameters (effective temperature, log g, microturbulent velocity) and the amplitude of pulsational variations on the derived elemental abundances.

  9. The anticonvulsant retigabine suppresses neuronal Kv2-mediated currents

    DEFF Research Database (Denmark)

    Stas, Jeroen I; Bocksteins, Elke; Jensen, Camilla S

    2016-01-01

    Enhancement of neuronal M-currents, generated through KV7.2-KV7.5 channels, has gained much interest for its potential in developing treatments for hyperexcitability-related disorders such as epilepsy. Retigabine, a KV7 channel opener, has proven to be an effective anticonvulsant and has recently...

  10. Photometric solution and frequency analysis of the oEA system EW Boo

    International Nuclear Information System (INIS)

    Zhang, X. B.; Wang, K.; Luo, Y. P.

    2015-01-01

    We present the first photometric solution and frequency analysis of the neglected oscillating Algol-type (oEA) binary EW Boo. B- and V-band light curves of the star were obtained on 11 nights in 2014. Using the Wilson–Devinney code, the eclipsing light curves were synthesized and the first photometric solution was derived for the binary system. The results reveal that EW Boo could be a semi-detached system with the less-massive secondary component filling its Roche lobe. By subtracting the eclipsing light changes from the data, we obtained the intrinsic pulsating light curves of the hotter, massive primary component. Frequency analysis of residual light shows multi-mode pulsation with the dominant period at 0.01909 days. A preliminary mode identification suggests that the star could be pulsating in non-radial (l = 1) modes. The long-term orbital period variation of the system was also investigated for the first time. An improved orbital period and new ephemerides of the eclipsing binary are given. The O−C analysis indicates a secular period increasing at a rate of dP/dt=2.9×10 −7 days yr −1 , which could be interpreted as mass transfer from the cooler secondary to the primary component.

  11. Liquid argon dielectric breakdown studies with the MicroBooNE purification system

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Carls, B.; James, C.; Johnson, B.; Jostlein, H.; Lockwitz, S.; Lundberg, B.; Raaf, J. L.; Rameika, R.; Rebel, B.; Zeller, G. P.; Zuckerbrot, M.

    2014-11-01

    The proliferation of liquid argon time projection chamber detectors makes the characterization of the dielectric properties of liquid argon a critical task. To improve understanding of these properties, a systematic study of the breakdown electric field in liquid argon was conducted using a dedicated cryostat connected to the MicroBooNE cryogenic system at Fermilab. An electrode sphere-plate geometry was implemented using spheres with diameters of 1.3 mm, 5.0 mm, and 76 mm. The MicroBooNE cryogenic system allowed measurements to be taken at a variety of electronegative contamination levels ranging from a few parts-per-million to tens of parts-per-trillion. The cathode-anode distance was varied from 0.1 mm to 2.5 cm. The results demonstrate a geometric dependence of the electric field strength at breakdown. This study is the first time that the dependence of the breakdown field on stressed cathode area has been shown for liquid argon.

  12. Two W-subtype contact binaries: GQ Boo and V1367 Tau

    Science.gov (United States)

    Zhang, Jia; Qian, Sheng-Bang; Han, Zhong-Tao; Wu, Yue

    2017-04-01

    Two contact binaries, GQ Boo and V1367 Tau, were observed and analysed with a new method to obtain the absolute parameters. The light-curve analysis shows that both of them are obvious W-subtype contact binaries, with much more massive but apparently cooler components (M2/M1 ≃ 2 and 4, T2/T1 ≃ 0.95 and 0.94). The orbital periods were studied using the O-C diagrams, and it is thought that the minima timings were heavily affected by the longstanding magnetic activities on the star surface, so the minima timings cannot represent the real period changes. The mass-radius relationships were proposed by the light-curve analysis alone, which is equivalent to the mean density. The density and temperature can determine the other absolute parameters in most of the time. With the almost complete star parameter space provided by PARSEC, approximate masses and radii were obtained (0.52 ± 0.08 M⊙ and 1.01 ± 0.15 M⊙ for GQ Boo, and 0.22 ± 0.01 M⊙ and 0.92 ± 0.06 M⊙ for V1367 Tau). The mass-radius relationship is a neglected useful tool to calculate the mass and radius, especially for the detached binaries.

  13. A COMPREHENSIVE PHOTOMETRIC STUDY OF THE CONTACT BINARY GN BOO WITH POSSIBLE MAGNETIC ACTIVITIES

    International Nuclear Information System (INIS)

    Wang, J. J.; Qian, S. B.; Zhang, J.; He, J. J.; Zhao, E. G.; Zhu, L. Y.; Liao, W. P.; Liu, L.; Zhang, Y. P.

    2015-01-01

    Multi-color photometric data of GN Boo observed from 2010 to 2013 are presented. The intrinsic variations of the light curves are remarkable, and their phenomena are probable evidence of stronger magnetic activities on the surfaces of the components. Based on all CCD times of minimum light, a secular increase superimposed on a cyclic oscillation is found. The orbital period increases at a rate of dP/dt = +1.74 × 10 −7 days yr −1 , which can be explained by mass transfer from the less massive component to the more massive one. The period and amplitude of the cyclic variation are P = 9.5632 yr and A = 0.0046 days, respectively, which correspond to the previous published results. Using the 2010 version of the W–D code, five sets of photometric solutions were derived from our new data. The results imply that the stellar spot, the degree of fill-out, and the temperature difference ΔT between the components of GN Boo are variable. It is inferred that the magnetic activities perhaps influence the outer radius of the component, causing the temperature of the component star and the level of contact to change

  14. Measurement of Reconstructed Charged Particle Multiplicities of Neutrino Interactions in MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Rafique, Aleena [Kansas State Univ., Manhattan, KS (United States)

    2017-09-25

    Here, we compare the observed charged particle multiplicity distributions in the MicroBooNE liquid argon time projection chamber from neutrino interactions in a restricted final state phase space to predictions of this distribution from several GENIE models. The measurement uses a data sample consisting of neutrino interactions with a final state muon candidate fully contained within the MicroBooNE detector. These data were collected in 2015-2016 with the Fermilab Booster Neutrino Beam (BNB), which has an average neutrino energy of 800 MeV, using an exposure corresponding to 5e19 protons-on-target. The analysis employs fully automatic event selection and charged particle track reconstruction and uses a data-driven technique to determine the contribution to each multiplicity bin from neutrino interactions and cosmic-induced backgrounds. The restricted phase space employed makes the measurement most sensitive to the higher-energy charged particles expected from primary neutrino-argon collisions and less sensitive to lower energy protons expected to be produced in final state interactions of collision products with the target argon nucleus.

  15. Peek-a-What? Infants' Response to the Still-Face Task after Normal and Interrupted Peek-a-Boo

    Science.gov (United States)

    Bigelow, Ann E.; Best, Caitlin

    2013-01-01

    Infants' sensitivity to the vitality or tension envelope within dyadic social exchanges was investigated by examining their responses following normal and interrupted games of peek-a-boo embedded in a Still-Face Task. Infants 5-6 months old engaged in two modified Still-Face Tasks with their mothers. In one task, the initial interaction ended with…

  16. Side Effects: An Analysis of Mind the Gap's "Boo" and the Reception of Theatre Involving Learning Disabled Actors

    Science.gov (United States)

    Hargrave, Matt

    2010-01-01

    This article analyses Mind the Gap's Boo, a re-imagining of Harper Lee's "To Kill a Mockingbird", which features a cast of learning disabled actors. It is concerned with the public reception of the work, particularly the "effect" of an all-disabled cast. What are the consequences, both ethical and aesthetic, for these actors to tell this story on…

  17. The subfamily-specific interaction between Kv2.1 and Kv6.4 subunits is determined by interactions between the N- and C-termini.

    Directory of Open Access Journals (Sweden)

    Elke Bocksteins

    Full Text Available The "silent" voltage-gated potassium (KvS channel subunit Kv6.4 does not form electrically functional homotetramers at the plasma membrane but assembles with Kv2.1 subunits, generating functional Kv2.1/Kv6.4 heterotetramers. The N-terminal T1 domain determines the subfamily-specific assembly of Kv1-4 subunits by preventing interactions between subunits that belong to different subfamilies. For Kv6.4, yeast-two-hybrid experiments showed an interaction of the Kv6.4 N-terminus with the Kv2.1 N-terminus, but unexpectedly also with the Kv3.1 N-terminus. We confirmed this interaction by Fluorescence Resonance Energy Transfer (FRET and co-immunoprecipitation (co-IP using N-terminal Kv3.1 and Kv6.4 fragments. However, full-length Kv3.1 and Kv6.4 subunits do not form heterotetramers at the plasma membrane. Therefore, additional interactions between the Kv6.4 and Kv2.1 subunits should be important in the Kv2.1/Kv6.4 subfamily-specificity. Using FRET and co-IP approaches with N- and C-terminal fragments we observed that the Kv6.4 C-terminus physically interacts with the Kv2.1 N-terminus but not with the Kv3.1 N-terminus. The N-terminal amino acid sequence CDD which is conserved between Kv2 and KvS subunits appeared to be a key determinant since charge reversals with arginine substitutions abolished the interaction between the N-terminus of Kv2.1 and the C-terminus of both Kv2.1 and Kv6.4. In addition, the Kv6.4(CKv3.1 chimera in which the C-terminus of Kv6.4 was replaced by the corresponding domain of Kv3.1, disrupted the assembly with Kv2.1. These results indicate that the subfamily-specific Kv2.1/Kv6.4 heterotetramerization is determined by interactions between Kv2.1 and Kv6.4 that involve both the N- and C-termini in which the conserved N-terminal CDD sequence plays a key role.

  18. RESOLVING THE ξ BOO BINARY WITH CHANDRA, AND REVEALING THE SPECTRAL TYPE DEPENDENCE OF THE CORONAL 'FIP EFFECT'

    International Nuclear Information System (INIS)

    Wood, Brian E.; Linsky, Jeffrey L.

    2010-01-01

    On 2008 May 2, Chandra observed the X-ray spectrum of ξ Boo (G8 V+K4 V), resolving the binary for the first time in X-rays and allowing the coronae of the two stars to be studied separately. With the contributions of ξ Boo A and B to the system's total X-ray emission now observationally established (88.5% and 11.5%, respectively), consideration of mass loss measurements for GK dwarfs of various activity levels (including one for ξ Boo) leads to the surprising conclusion that ξ Boo B may dominate the wind from the binary, with ξ Boo A's wind being very weak despite its active corona. Emission measure (EM) distributions and coronal abundances are computed for both stars and compared with Chandra measurements of other moderately active stars with G8-K5 spectral types, all of which exhibit a narrow peak in EM near log T = 6.6, indicating that the coronal heating process in these stars has a strong preference for this temperature. As is the case for the Sun and many other stars, our sample of stars shows coronal abundance anomalies dependent on the first ionization potential (FIP) of the element. We see no dependence of the degree of 'FIP effect' on activity, but there is a dependence on spectral type, a correlation that becomes more convincing when moderately active main-sequence stars with a broader range of spectral types are considered. This clear dependence of coronal abundances on spectral type weakens if the stellar sample is allowed to be contaminated by evolved stars, interacting binaries, or extremely active stars with log L X >29, explaining why this correlation has not been recognized in the past.

  19. Resolving the xi Boo Binary with Chandra, and Revealing the Spectral Type Dependence of the Coronal "Fip Effect"

    Science.gov (United States)

    Wood, Brian E.; Linsky, Jeffrey L.

    2010-01-01

    On 2008 May 2, Chandra observed the X-ray spectrum of xi Boo (G8 V+K4 V), resolving the binary for the first time in X-rays and allowing the coronae of the two stars to be studied separately. With the contributions of ξ Boo A and B to the system's total X-ray emission now observationally established (88.5% and 11.5% respectively), consideration of mass loss measurements for GK dwarfs of various activity levels (including one for xi Boo) leads to the surprising conclusion that xi Boo B may dominate the wind from the binary, with xi Boo A's wind being very weak despite its active corona. Emission measure (EM) distributions and coronal abundances are computed for both stars and compared with Chandra measurements of other moderately active stars with G8-K5 spectral types, all of which exhibit a narrow peak in EM near log T = 6.6, indicating that the coronal heating process in these stars has a strong preference for this temperature. As is the case for the Sun and many other stars, our sample of stars shows coronal abundance anomalies dependent on the first ionization potential (FIP) of the element. We see no dependence of the degree of FIP effect on activity, but there is a dependence on spectral type, a correlation that becomes more convincing when moderately active main-sequence stars with a broader range of spectral types are considered. This clear dependence of coronal abundances on spectral type weakens if the stellar sample is allowed to be contaminated by evolved stars, interacting binaries or extremely active stars with logLX 29, explaining why this correlation has not been recognized in the past.

  20. Effects of haloperidol on Kv4.3 potassium channels.

    Science.gov (United States)

    Lee, Hong Joon; Sung, Ki-Wug; Hahn, Sang June

    2014-10-05

    Haloperidol is commonly used in clinical practice to treat acute and chronic psychosis, but it also has been associated with adverse cardiovascular events. We investigated the effects of haloperidol on Kv4.3 currents stably expressed in CHO cells using a whole-cell patch-clamp technique. Haloperidol did not significantly inhibit the peak amplitude of Kv4.3, but accelerated the decay rate of inactivation of Kv4.3 in a concentration-dependent manner. Thus, the effects of haloperidol on Kv4.3 were estimated from the integral of the Kv4.3 currents during the depolarization pulse. The Kv4.3 was decreased by haloperidol in a concentration-dependent manner with an IC50 value of 3.6 μM. Haloperidol accelerated the decay rate of Kv4.3 inactivation and activation kinetics in a concentration-dependent manner, thereby decreasing the time-to-peak. Haloperidol shifted the voltage dependence of the steady-state activation and inactivation of Kv4.3 in a hyperpolarizing direction. Haloperidol also caused an acceleration of the closed-state inactivation of Kv4.3. Haloperidol produced a use-dependent block of Kv4.3, which was accompanied by a slowing of recovery from the inactivation of Kv4.3. These results suggest that haloperidol blocks Kv4.3 by both interacting with the open state of Kv4.3 channels during depolarization and accelerating the closed-state inactivation at subthreshold membrane potentials. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

    Directory of Open Access Journals (Sweden)

    Xuan Zhang, Yue-feng Chu, Ping Zhao, Peng-cheng Gao, Ying He, Nu Wang and Zhong-xin Lu*

    2013-11-01

    Full Text Available Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E.coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease.

  2. Kv7 channels can function without constitutive calmodulin tethering.

    Directory of Open Access Journals (Sweden)

    Juan Camilo Gómez-Posada

    Full Text Available M-channels are voltage-gated potassium channels composed of Kv7.2-7.5 subunits that serve as important regulators of neuronal excitability. Calmodulin binding is required for Kv7 channel function and mutations in Kv7.2 that disrupt calmodulin binding cause Benign Familial Neonatal Convulsions (BFNC, a dominantly inherited human epilepsy. On the basis that Kv7.2 mutants deficient in calmodulin binding are not functional, calmodulin has been defined as an auxiliary subunit of Kv7 channels. However, we have identified a presumably phosphomimetic mutation S511D that permits calmodulin-independent function. Thus, our data reveal that constitutive tethering of calmodulin is not required for Kv7 channel function.

  3. Orbital period variations of two W UMa-type binaries: UY UMa and EF Boo

    Science.gov (United States)

    Yu, Yun-Xia; Zhang, Xu-Dong; Hu, Ke; Xiang, Fu-Yuan

    2017-08-01

    The orbital period variations of two W UMa-type contact binaries, UY UMa and EF Boo, are analyzed by using all available times of light minimum. It is detected that the general trends of their (O - C) curves show an upward parabolic variation, which reveals their continuous period increases at the rates of dP / dt = 2.545 ×10-7 days yr-1 and dP / dt = 2.623 ×10-7 days yr-1 , respectively. Meanwhile, UY UMa also shows a cyclic period variation with a small amplitude of A = 0.0026 days superposed on the long-term increase. Due to their contact configurations, the secular period increases are interpreted as a result of mass transfer from the less massive component to the more massive one. The cyclic period variation of UY UMa may be interpreted in terms of either the magnetic activity or the light time effect.

  4. Search for dark companions of K and M giants - Preliminary results for Alpha Boo

    Science.gov (United States)

    Heyer, I.; Hall, D. N. B.; Hinkle, K.

    1987-01-01

    Preliminary results are presented for Alpha Boo, encompassing selected measurements during the period from January 1979 to June 1984. Four groups of data are chosen, each group taken within an interval of up to 1.5 months, and examined the internal consistency of each set to look for short term trends. The radial velocity was found to be very consistent with a standard deviation of about + or - 50 m/s within time scales of hours, days, and weeks. No significant radial velocity changes over these periods were detected. This suggests that the stellar surface activity is confined to the level of uncertainty. Because of this consistency it was possible to take the means of the radial velocity measurements within each group and compare them to look for possible long term changes. Variations of the radial velocity of about 200 m/s were found over the span of a few years, significant at the 6 to 8 sigma level.

  5. VizieR Online Data Catalog: AQ Boo VRI differential light curves (Wang+, 2016)

    Science.gov (United States)

    Wang, S.; Zhang, L.; Pi, Q.; Han, X. L.; Zhang, X.; Lu, H.; Wang, D.; Li, T.

    2016-11-01

    On March 22 and April 19 in 2014, we observed AQ Boo with the 60cm telescope at Xinglong Station of the National Astronomical Observatories of China (NAOC). The CCD camera on this telescope has a resolution of 1024 x 1024 pixels and its corresponding field of view is 17'x17' (Yang, 2013NewA...25..109Y). The other three days of data were obtained using the 1-m telescope at Yunnan Observatory of Chinese Academy of Sciences, on January 20, 21 and February 28 in 2015. The CCD camera on this telescope has a resolution of 2048x2048 pixels and its corresponding field of view is 7.3'x7.3'. Bessel VRI filters were used. The exposure times are 100-170s, 50-100s and 50-80s in the V, R, I bands, respectively. (1 data file).

  6. Automated Proton Track Identification in MicroBooNE Using Gradient Boosted Decision Trees

    Energy Technology Data Exchange (ETDEWEB)

    Woodruff, Katherine [New Mexico State U.

    2017-10-02

    MicroBooNE is a liquid argon time projection chamber (LArTPC) neutrino experiment that is currently running in the Booster Neutrino Beam at Fermilab. LArTPC technology allows for high-resolution, three-dimensional representations of neutrino interactions. A wide variety of software tools for automated reconstruction and selection of particle tracks in LArTPCs are actively being developed. Short, isolated proton tracks, the signal for low- momentum-transfer neutral current (NC) elastic events, are easily hidden in a large cosmic background. Detecting these low-energy tracks will allow us to probe interesting regions of the proton's spin structure. An effective method for selecting NC elastic events is to combine a highly efficient track reconstruction algorithm to find all candidate tracks with highly accurate particle identification using a machine learning algorithm. We present our work on particle track classification using gradient tree boosting software (XGBoost) and the performance on simulated neutrino data.

  7. An improved Neutrino Oscillations Analysis of the MiniBooNE Data

    International Nuclear Information System (INIS)

    Aguilar-Arevalo, Alexis Armando

    2008-01-01

    We calculate the exclusion region in the parameter space of ν μ → ν e oscillations of the LSND type using a combined fit to the reconstructed energy distributions of neutrino candidate samples from the MiniBooNE data obtained with two different particle identification methods. The two ν e candidate samples are included together with a high statistics sample of ν μ events in the definition of a χ 2 statistic which includes the correlations between the energy intervals of all three samples and handles the event overlap between the ν e samples. The ν μ sample is introduced to constrain the effect of systematic uncertainties. This analysis increases the exclusion limit in the region Δm 2 ∼ 2 when compared with the result previously published by the collaboration, which used a different technique

  8. Urine flow acceleration is superior to Qmax in diagnosing BOO in patients with BPH.

    Science.gov (United States)

    Wen, Jian-guo; Cui, Lin-gang; Li, Yi-dong; Shang, Xiao-ping; Zhu, Wen; Zhang, Rui-li; Meng, Qing-jun; Zhang, Sheng-jun

    2013-08-01

    We performed a retrospective, case-control study to evaluate whether the urine flow acceleration (UFA, mL/s(2)) is superior to maximum uroflow (Qmax, mL/s) in diagnosing bladder outlet obstruction (BOO) in patients with benign prostatic hyperplasia (BPH). In this study, a total of 50 men with BPH (age: 58±12.5 years) and 50 controls (age: 59±13.0 years) were included. A pressure-flow study was used to determine the presence of BOO according to the recommendations of Incontinence Control Society (ICS). The results showed that the UFA and Qmax in BPH group were much lower than those in the control group [(2.05±0.85) vs. (4.60±1.25) mL/s(2) and (8.50±1.05) vs. (13.00±3.35) mL/s] (PBOO were 88%, 75% vs. 81%, 63%. UFA vs. Omax, when compared with the results of P-Q chart (the kappa values in corresponding analysis), was 0.55 vs. 0.35. The prostate volume, post void residual and detrusor pressure at Qmax between the two groups were 28.6±9.8 vs. 24.2±7.6 mL, 60.4±1.4 vs. 21.3±2.5 mL and 56.6±8.3 vs. 21.7±6.1 cmH2O, respectively (PBOO in patients with BPH.

  9. Efficacy of holmium laser enucleation of the prostate (HoLEP) in men with bladder outlet obstruction (BOO) and non-neurogenic bladder dysfunction.

    Science.gov (United States)

    Pyun, Jong Hyun; Kang, Sung Gu; Kang, Seok Ho; Cheon, Jun; Kim, Je Jong; Lee, Jeong Gu

    2017-09-01

    We aimed to compare the short-term outcomes of men who had urodynamic evidence of detrusor underactivity (DU) or detrusor overactivity (DO) of a non-neurogenic etiology as well as bladder outlet obstruction (BOO) and who underwent Holmium Laser Enucleation of the prostate (HoLEP). A database of 322 patients who underwent HoLEP between 2010 and 2014 was analyzed. Patients were classified into three groups according to the results of a preoperative urodynamic study. Preoperative parameters such as International Prostate Symptom Score (IPSS), Quality of Life (QoL) index, IPSS grade, uroflowmetry were compared with postoperative parameters measured at 6 months. There were 138 patients with BOO-only and 89 patients with BOO and detrusor dysfunction including 56 with DO and 33 with DU. The degree of improvement in IPSS-total (BOO: 10.7, DO: 8.3, DU: 7.0; p = 0.023) was greater in the BOO-only group than in the DU group. There were more patients whose IPSS grade improved in the BOO-only group (71%) than in the detrusor dysfunction group (DO: 53.6% and DU: 45.5%). Postoperative IPSS-voiding (4.5 vs 7.0), and Qmax (18 vs 13.7) in the BOO-only group were significantly better than those in the DU group. Additionally, postoperative IPSS-storage (4.7 vs 6.7), and IPSS-total (9.1 vs 12.3) in the BOO-only group were significantly better than in the DO group (all p < 0.05). In conclusion, early surgical management for men with severe LUTS and associated BPH before secondary degeneration occurs may be beneficial for preserving detrusor function and yield better treatment outcomes. Copyright © 2017. Published by Elsevier Taiwan.

  10. Molecular and functional characterization of Kv7 K+ channel in murine gastrointestinal smooth muscles

    DEFF Research Database (Denmark)

    Jepps, Thomas Andrew; Greenwood, Iain A; Moffatt, James D

    2009-01-01

    that K(v)7.x especially K(v)7.4 and K(v)7.5 are expressed in different regions of the murine gastrointestinal tract and blockers of K(v)7 channels augment inherent contractile activity. Drugs that selectively block K(v)7.4/7.5 might be promising therapeutics for the treatment of motility disorders...

  11. Long-term orbital period behaviour of low mass ratio contact binaries GR Vir and FP Boo

    Science.gov (United States)

    Ćetinkaya, Halil; Soydugan, Faruk

    2017-02-01

    In this study, we investigated orbital period variations of two low mass ratio contact binaries GR Vir and FP Boo based on published minima times. From the O-C analysis, it was found that FP Boo indicates orbital period decrease while the period of GR Vir is increasing. Mass transfer process was used to explain increase and decrease in the orbital periods. In the O-C diagrams of both systems periodic variations also exist. Cyclic changes can be explained as being the result of a light-travel time effect via a third component around the eclipsing binaries. In order to interpret of cyclic orbital period changes for GR Vir, which has late-type components, possible magnetic activity cycles of the components have been also considered.

  12. Coronal Abundances In Xi Boo A, Abundances in the Corona of FK Aqr and the Origin of Cosmic Ray

    Science.gov (United States)

    Drake, Jeremy; Oliversen, Ronald J. (Technical Monitor)

    2002-01-01

    Both Xi Boo A and FK Agr observations have been acquired, reduced and analysed. The results of the Xi Boo A study were published in ApJ (Drake, J.J., Kashyap, V., 2001, Up, 547, 428). Work is ongoing to determine the coronet metallicity of FK Aqr based on EUVE spectra and DO photometer data, using the techniques developed in the Drake & Kashyap paper. Preliminary synthetic spectra for FK Aqr have been developed, and further work to heap define the hot plasma emission measure distribution is underway. Progress on spectral analysis has been hampered following the post-doctoral scientist, Vinay Kashyap, taking up new position. A suitable replacement has been difficult to find, but new hire is expected shortly. However, good progress has been made on analysis of DO photometric variations in FK Age, with a publication now ready (Kashyap, V., Drake, J.J., Guedel, M., Audard, M.) for submission to a mainstream astrophysical journal.

  13. Assessing the role of nuclear effects in the interpretation of the MiniBooNE low-energy anomaly

    CERN Document Server

    Ericson, M; Giunti, C; Martini, M

    2016-01-01

    We study the impact of the effect of multinucleon interactions in the reconstruction of the neutrino energy on the fit of the MiniBooNE data in terms of neutrino oscillations. We obtain some improvement of the fit of the MiniBooNE low-energy excess in the framework of two-neutrino oscillations and a shift of the allowed region in the $\\sin^2 2\\vartheta$--$\\Delta{m}^2$ plane towards smaller values of $\\sin^2 2\\vartheta$ and larger values of $\\Delta{m}^2$. However this effect is not enough to solve the problem of the appearance-disappearance tension in the global fit of short-baseline neutrino oscillation data.

  14. Assessing the role of nuclear effects in the interpretation of the MiniBooNE low-energy anomaly

    Science.gov (United States)

    Ericson, M.; Garzelli, M. V.; Giunti, C.; Martini, M.

    2016-04-01

    We study the impact of the effect of multinucleon interactions in the reconstruction of the neutrino energy on the fit of the MiniBooNE data in terms of neutrino oscillations. We obtain some improvement of the fit of the MiniBooNE low-energy excess in the framework of two-neutrino oscillations and a shift of the allowed region in the sin22 ϑ -Δ m2 plane toward smaller values of sin22 ϑ and larger values of Δ m2. However, this effect is not enough to solve the problem of the appearance-disappearance tension in the global fit of short-baseline neutrino oscillation data.

  15. Assessing the role of nuclear effects in the interpretation of the MiniBooNE low-energy anomaly

    Energy Technology Data Exchange (ETDEWEB)

    Ericson, M. [Lyon 1 Univ., CNRS/IN2P3 (France). INP Lyon; CERN Geneva (Switzerland). Theory Unit; Garzelli, M.V. [Hamburg Univ. (Germany). 2. Inst. fuer Theoretische Physik; Giunti, C. [INFN, Sezione di Torino (Italy); Martini, M. [ESNT, CEA-Saclay, IRFU, Gif-sur-Yvette (France). Service de Physique Nucleaire

    2016-02-15

    We study the impact of the effect of multinucleon interactions in the reconstruction of the neutrino energy on the fit of the MiniBooNE data in terms of neutrino oscillations. We obtain some improvement of the fit of the MiniBooNE low-energy excess in the framework of two-neutrino oscillations and a shift of the allowed region in the sin{sup 2}2θ-Δm{sup 2} plane towards smaller values of sin{sup 2}2ν and larger values of Δm{sup 2}. However this effect is not enough to solve the problem of the appearance-disappearance tension in the global fit of short-baseline neutrino oscillation data.

  16. Assessing the role of nuclear effects in the interpretation of the MiniBooNE low-energy anomaly

    International Nuclear Information System (INIS)

    Ericson, M.; Martini, M.

    2016-02-01

    We study the impact of the effect of multinucleon interactions in the reconstruction of the neutrino energy on the fit of the MiniBooNE data in terms of neutrino oscillations. We obtain some improvement of the fit of the MiniBooNE low-energy excess in the framework of two-neutrino oscillations and a shift of the allowed region in the sin 2 2θ-Δm 2 plane towards smaller values of sin 2 2ν and larger values of Δm 2 . However this effect is not enough to solve the problem of the appearance-disappearance tension in the global fit of short-baseline neutrino oscillation data.

  17. Analysis of the 35 KV substation secondary system

    Science.gov (United States)

    Zhao, Yong; Jiang, Jianguo; Jiang, Chunlei; Ren, Shuang; Liu, Songbin

    2017-04-01

    This paper analyzes the status of the two system of some 35KV users' substation in Daqing oil field, the deficiencies of the two system of the existing 35KV substation are found out. And put forward the opinion of acceptance in the future work. I hope it can able to work in the future on the protection of professional help.

  18. Immunocytochemical localization of the elongation factor Tu in E. coli cells

    NARCIS (Netherlands)

    Slot, J.W.; Schilstra, M.J.; Meide, P.H. van der; Posthuma, G.; Cremers, A.F.M.; Bosch, L.

    1984-01-01

    The localization of the elongation factor Tu (EF-Tu) in ultrathin cryosections of E. coli cells was determined with the electron microscope using a highly specific immunological labellin technique. EF-Tu is distributed almost homogeneously throughout the cytoplasm. Although it has often been

  19. Functional studies of elongation factor Tu from Escherichia coli : Site-directed mutagenesis and antibiotic action

    NARCIS (Netherlands)

    Krab, Ivo Maarten

    2001-01-01

    This PhD thesis describes several studies into the structure and function of Escherichia coli Elongation Factor Tu (EF-Tu). EF-Tu plays a central role in the bacterial protein synthesis machinery as the carrier of "coded building blocks" for protein synthesis, aminoacylated tRNA (aa-tRNA). Without

  20. Trust Management in P2P systems using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  1. Trust management in P2P systems using standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.; Doumen, J.M.; Etalle, S.; Karabulut, Y.; Mitchell, J.C.; Herrmann, P.; Jensen, C.D.

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  2. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  3. The role of intravesical prostatic protrusion (IPP) in the evaluation and treatment of bladder outlet obstruction (BOO).

    Science.gov (United States)

    Kuo, Tricia L C; Teo, Jonathan S M; Foo, K T

    2016-04-01

    Intravesical prostatic protrusion (IPP) may be an underutilized modality for the assessment of bladder outlet obstruction (BOO). Pressure flow studies or urodynamics have long been the gold standard for the evaluation of lower urinary tract symptoms (LUTS) in men but are invasive, time-consuming and costly. Potentially, IPP may be a useful adjunct prior to performing urodynamics. Measurement of IPP is taken in the sagittal view, using the transabdominal ultrasound. It is the vertical height from the tip of the protrusion to the base of the prostate. This technique was previously described. We reviewed previous publications that studied the accuracy, positive predictive value and clinical use of IPP. In addition, we noted the comments regarding the challenges of using this technique. IPP has been shown to have a positive predictive value of 72% for BOO. It has been calculated to have an area under curve (AUC) value of 0.71 and 0.84 in some stuies. Clinically, it may be used to predict the outcome of a trial without catheter following acute retention of urine. Patients with higher IPP grade were noted to have a higher risk of clinical progression. Studies have also shown that men with higher IPP are poorer responders to medical treatment such as α-blockers. Compared to other modalities, the advantage of IPP in assessing BOO may be its easy applicability and non-invasive nature. Therefore, there is a consideration for a larger role of IPP in bedside assessment and management of BOO in daily practice. Neurourol. Urodynam. 35:535-537, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

  4. Measurement of the absolute vμ-CCQE cross section at the SciBooNE experiment

    Energy Technology Data Exchange (ETDEWEB)

    Aunion, Jose Luis Alcaraz [Autonomous Univ. of Barcelona (Spain)

    2010-07-01

    This thesis presents the measurement of the charged current quasi-elastic (CCQE) neutrino-nucleon cross section at neutrino energies around 1 GeV. This measurement has two main physical motivations. On one hand, the neutrino-nucleon interactions at few GeV is a region where existing old data are sparse and with low statistics. The current measurement populates low energy regions with higher statistics and precision than previous experiments. On the other hand, the CCQE interaction is the most useful interaction in neutrino oscillation experiments. The CCQE channel is used to measure the initial and final neutrino fluxes in order to determine the neutrino fraction that disappeared. The neutrino oscillation experiments work at low neutrino energies, so precise measurement of CCQE interactions are essential for flux measurements. The main goal of this thesis is to measure the CCQE absolute neutrino cross section from the SciBooNE data. The SciBar Booster Neutrino Experiment (SciBooNE) is a neutrino and anti-neutrino scattering off experiment. The neutrino energy spectrum works at energies around 1 GeV. SciBooNE was running from June 8th 2007 to August 18th 2008. In that period, the experiment collected a total of 2.65 x 1020 protons on target (POT). This thesis has used full data collection in neutrino mode 0.99 x 1020 POT. A CCQE selection cut has been performed, achieving around 70% pure CCQE sample. A fit method has been exclusively developed to determine the absolute CCQE cross section, presenting results in a neutrino energy range from 0.2 to 2 GeV. The results are compatible with the NEUT predictions. The SciBooNE measurement has been compared with both Carbon (MiniBoonE) and deuterium (ANL and BNL) target experiments, showing a good agreement in both cases.

  5. TU PATERE LEGEM QUAM IPSE FECISTI

    CERN Multimedia

    Association du personnel

    2010-01-01

    In two judgments of February 2009, the ILO Administrative Tribunal (hereafter ILOAT) upheld CERN’s contract policy. In another case having given rise to a recent judgment (3 February 2010), the same problems, in essence, were raised concerning the illegal injustice which could result from the implementation of the policy in question. This time, the Tribunal decided in favour of our colleague, basing itself on the Latin maxim tu patere legem quam ipse fecisti, according to which any authority is bound by its own rules, as long as such rules have not been amended or abrogated. In fact, the Administration had informed the complainant that his application for a long-term contract would be examined for six slots, when in fact it was examined for only one. This judgment represents a victory for the complainant, for the Staff Association and its perseverance, and also for the principle according to which any organisation must keep its word and act in good faith. It shows that, without reversing its jud...

  6. A measurement of the neutral current neutrino-nucleon elastic cross section at MiniBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Cox, David Christopher [Indiana Univ., Bloomington, IN (United States)

    2008-02-01

    The neutral current neutrino-nucleon elastic interaction v N → v N is a fundamental process of the weak interaction ideally suited for characterizing the structure of the nucleon neutral weak current. This process comprises ~18% of neutrino events in the neutrino oscillation experiment, MiniBooNE, ranking it as the experiment's third largest process. Using ~10% of MiniBooNE's available neutrino data, a sample of these events were identified and analyzed to determine the differential cross section as a function of the momentum transfer of the interaction, Q2. This is the first measurement of a differential cross section with MiniBooNE data. From this analysis, a value for the nucleon axial mass MA was extracted to be 1.34 ± 0.25 GeV consistent with previous measurements. The integrated cross section for the Q2 range 0.189 → 1.13 GeV2 was calculated to be (8.8 ± 0.6(stat) ± 0.2(syst)) x 10-40 cm2.

  7. BOO-1137-AN EXTREMELY METAL-POOR STAR IN THE ULTRA-FAINT DWARF SPHEROIDAL GALAXY BOOeTES I

    International Nuclear Information System (INIS)

    Norris, John E.; Yong, David; Gilmore, Gerard; Wyse, Rosemary F. G.

    2010-01-01

    We present high-resolution (R ∼ 40,000), high-signal-to-noise ratio (20-90) spectra of an extremely metal-poor giant star Boo-1137 in the 'ultra-faint' dwarf spheroidal galaxy (dSph) Booetes I, absolute magnitude M V ∼ -6.3. We derive an iron abundance of [Fe/H] = -3.7, making this the most metal-poor star as yet identified in an ultra-faint dSph. Our derived effective temperature and gravity are consistent with its identification as a red giant in Booetes I. Abundances for a further 15 elements have also been determined. Comparison of the relative abundances, [X/Fe], with those of the extremely metal-poor red giants of the Galactic halo shows that Boo-1137 is 'normal' with respect to C and N, the odd-Z elements Na and Al, the iron-peak elements, and the neutron-capture elements Sr and Ba, in comparison with the bulk of the Milky Way halo population having [Fe/H] ∼<-3.0. The α-elements Mg, Si, Ca, and Ti are all higher by Δ[X/Fe] ∼ 0.2 than the average halo values. Monte Carlo analysis indicates that Δ[α/Fe] values this large are expected with a probability ∼0.02. The elemental abundance pattern in Boo-1137 suggests inhomogeneous chemical evolution, consistent with the wide internal spread in iron abundances we previously reported. The similarity of most of the Boo-1137 relative abundances with respect to halo values, and the fact that the α-elements are all offset by a similar small amount from the halo averages, points to the same underlying galaxy-scale stellar initial mass function, but that Boo-1137 likely originated in a star-forming region where the abundances reflect either poor mixing of supernova (SN) ejecta, or poor sampling of the SN progenitor mass range, or both.

  8. Reconstitution of Qbeta RNA replicase from a covalently bonded elongation factor Tu-Ts complex

    DEFF Research Database (Denmark)

    Brown, S; Blumenthal, T

    1976-01-01

    of these polypeptides, protein synthesis elongation factors EF-Tu and EF-Ts, can be covalently crosslinked with dimethyl suberimidate to form a complex which lacks the ability to catalyze the known host functions catalyzed by the individual elongation factors. Using a previously developed reconstitution system we have...... examined the effects of crosslinking the EF-Tu-Ts complex on reconstituted replicase activity. Renaturation is significantly more efficient when exogenously added native EF-Tu-Ts is crosslinked than when it is not. Crosslinked EF-Tu-Ts can be purified from a crude crosslinked postribosomal supernatant...... by its ability to replace EF-Tu and EF-Ts in the renaturation of denatured Qbeta replicase. A sample of Qbeta replicase with crosslinked EF-Tu-Ts replacing the individual elongation factors was prepared. Although it lacked EF-Tu and EF-Ts activities, it could initiate transcription of both poly...

  9. Purification, characterization, and sequencing of novel antimicrobial peptides, Tu-AMP 1 and Tu-AMP 2, from bulbs of tulip (Tulipa gesneriana L.).

    Science.gov (United States)

    Fujimura, Masatoshi; Ideguchi, Mineo; Minami, Yuji; Watanabe, Keiichi; Tadera, Kenjiro

    2004-03-01

    Novel antimicrobial peptides (AMP), designated Tu-AMP 1 and Tu-AMP 2, were purified from the bulbs of tulip (Tulipa gesneriana L.) by chitin affinity chromatography and reverse-phase high-performance liquid chromatography (HPLC). They bind to chitin in a reversible way. They were basic peptides having isoelectric points of over 12. Tu-AMP 1 and Tu-AMP 2 had molecular masses of 4,988 Da and 5,006 Da on MALDI-TOF MS analysis, and their extinction coefficients of 1% aqueous solutions at 280 nm were 3.3 and 3.4, respectively. Half of all amino acid residues of Tu-AMP 1 and Tu-AMP 2 were occupied by cysteine, arginine, lysine, and proline. The concentrations of peptides required for 50% inhibition (IC(50)) of the growth of plant pathogenic bacteria and fungi were 2 to 20 microg/ml. The structural characteristics of Tu-AMP 1 and Tu-AMP 2 indicated that they were novel thionin-like antimicrobial peptides, though Tu-AMP 2 was a heterodimer composes of two short peptides joined with disulfide bonds.

  10. Reconciling results of LSND, MiniBooNE and other experiments with soft decoherence

    CERN Document Server

    Farzan, Yasaman; Smirnov, Alexei Yu

    2008-01-01

    We propose an explanation of the LSND signal via quantum-decoherence of the mass states, which leads to damping of the interference terms in the oscillation probabilities. The decoherence parameters as well as their energy dependence are chosen in such a way that the damping affects only oscillations with the large (atmospheric) $\\Delta m^2$ and rapidly decreases with the neutrino energy. This allows us to reconcile the positive LSND signal with MiniBooNE and other null-result experiments. The standard explanations of solar, atmospheric, KamLAND and MINOS data are not affected. No new particles, and in particular, no sterile neutrinos are needed. The LSND signal is controlled by the 1-3 mixing angle $\\theta_{13}$ and, depending on the degree of damping, yields $0.0014 < \\sin^2\\theta_{13} < 0.034$ at $3\\sigma$. The scenario can be tested at upcoming $\\theta_{13}$ searches: while the comparison of near and far detector measurements at reactors should lead to a null-result a positive signal for $\\theta_{13...

  11. Study of Electromagnetic Interactions in the MicroBooNE Liquid Argon Time Projection Chamber

    Energy Technology Data Exchange (ETDEWEB)

    Caratelli, David [Columbia U.

    2018-01-01

    This thesis presents results on the study of electromagnetic (EM) activity in the MicroBooNE Liquid Argon Time Projection Chamber (LArTPC) neutrino detector. The LArTPC detector technology provides bubble-chamber like information on neutrino interaction final states, necessary to perform precision measurements of neutrino oscillation parameters. Accelerator-based oscillation experiments heavily rely on the appearance channel ! e to make such measurements. Identifying and reconstructing the energy of the outgoing electrons from such interactions is therefore crucial for their success. This work focuses on two sources of EM activity: Michel electrons in the 10-50 MeV energy range, and photons from 0 decay in the 30-300 MeV range. Studies of biases in the energy reconstruction measurement, and energy resolution are performed. The impact of shower topology at different energies is discussed, and the importance of thresholding and other reconstruction effects on producing an asymmetric and biased energy measurement are highlighted. This work further presents a study of the calorimetric separation of electrons and photons with a focus on the shower energy dependence of the separation power.

  12. Charged current antineutrino reactions from 12C at MiniBooNE energies

    International Nuclear Information System (INIS)

    Athar, M. Sajjad; Ahmad, Shakeb; Singh, S. K.

    2007-01-01

    A study of charged current induced antineutrino interactions from nuclei has been done for the intermediate energy antineutrinos and applied to 12 C, relevant for ongoing experiment by MiniBooNE collaboration. The calculations have been done for the quasielastic and inelastic lepton production as well as for the incoherent and the coherent pion production processes. The calculations are done in local density approximation. In the case of the quasielastic reaction the effects of Pauli blocking, Fermi motion effects, renormalization of weak transition strengths in nuclear medium and the Coulomb distortion of the outgoing lepton have been taken into account. For the inelastic processes the calculations have been done in the Δ dominance model and take into account the effect of Pauli blocking, Fermi motion of the nucleon, and renormalization of Δ properties in a nuclear medium. The effect of final state interactions of pions is also taken into account. The numerical results for the total cross sections for the charged current quasielastic scattering and incoherent pion production processes are compared with earlier experimental results available in freon and freon-propane. It is found that nuclear medium effects give strong reduction in the cross sections leading to satisfactory agreement with the available data

  13. Chemical Composition of RR Lyn - an Eclipsing Binary System with Am and λ Boo Type Components

    Science.gov (United States)

    Jeong, Yeuncheol; Yushchenko, Alexander V.; Doikov, Dmytry N.; Gopka, Vira F.; Yushchenko, Volodymyr O.

    2017-06-01

    High-resolution spectroscopic observations of the eclipsing binary system RR Lyn were made using the 1.8 m telescope at the Bohuynsan Optical Astronomical Observatory in Korea. The spectral resolving power was R = 82,000, with a signal to noise ratio of S/N > 150. We found the effective temperatures and surface gravities of the primary and secondary components to be equal to Teff = 7,920 & 7,210 K and log(g) = 3.80 & 4.16, respectively. The abundances of 34 and 17 different chemical elements were found in the atmospheric components. Correlations between the derived abundances with condensation temperatures and the second ionization potentials of these elements are discussed. The primary component is a typical metallic line star with the abundances of light and iron group elements close to solar values, while elements with atomic numbers Z > 30 are overabundant by 0.5-1.5 dex with respect to solar values. The secondary component is a λ Boo type star. In this type of stars, CNO abundances are close to solar values, while the abundance pattern shows a negative correlation with condensation temperatures.

  14. An improved Neutrino Oscillations Analysis of the MiniBooNE Data

    Energy Technology Data Exchange (ETDEWEB)

    Aguilar-Arevalo, Alexis Armando [Columbia Univ., New York, NY (United States)

    2008-01-01

    We calculate the exclusion region in the parameter space of vμ → v oscillations of the LSND type using a combined fit to the reconstructed energy distributions of neutrino candidate samples from the MiniBooNE data obtained with two different particle identification methods. The two ve candidate samples are included together with a high statistics sample of vμ events in the definition of a X2 statistic which includes the correlations between the energy intervals of all three samples and handles the event overlap between the ve samples. The vμ sample is introduced to constrain the effect of systematic uncertainties. This analysis increases the exclusion limit in the region Δm2≲ 1eV2 when compared with the result previously published by the collaboration, which used a different technique.

  15. Measurement of neutrino induced charged current neutral pion production cross section at SciBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Catala-Perez, Juan [Univ. of Valencia (Spain)

    2014-01-01

    SciBooNE is a neutrino scattering experiment located in the Booster Neutrino Beam at Fermilab. It collected data from June 2007 to August 2008 to accurately measure muon neutrino and anti-neutrino cross sections on carbon around 1 GeV neutrino energy. In this thesis we present the results on the measurement of the muon neutrino cross section resulting in a μ- plus a single π0 final state (CC- π0 channel). The present work will show the steps taken to achieve this result: from the reconstruction improvements to the background extraction. The flux-averaged CC - π0 production cross section measurement obtained in this thesis < σCC- π0 > Φ = (5.6 ± 1.9fit ± 0.7beam ± 0.5int - 0.7det) × 10-40 cm2/N at an average energy of 0.89 GeV is found to agree well both with the expectation from the Monte Ca

  16. [The story of K.V. Tjellesen].

    Science.gov (United States)

    Clemmensen, Peter

    2005-01-01

    This is the story about a Danish pharmaceutical wholesaler. The story begins in 1909 in some basement rooms located in Vester Boulevard 42 (which was later to become the Boulevard of H.C. Andersen) in Copenhagen. The founder of the company, pharmacist Knud Valdemar Tjellesen, lived in the very same building. In the beginning, the company mainly sold chemicals and produced and sold chemical-technical products. In 1930, K.V. Tjellesen became the proprietor of the pharmacy Sct. Johannes Apotek situated in Fredensgade 5, Nørrebro in Copenhagen. The company then moved into some offices that were located just behind the pharmacy. Already in 1918, K.V. Tjellesen traded pharmaceutical specialties, which were imported, to a certain extent, from Germany through a purchasing office in Hamburg. In 1938, the son of Knud Valdemar Tjellesen, pharmacist Paul Tjellesen, joined the wholesale company charged with the primary task of intensifying the sale of the company's international agency products. At the same time, the general partnership K.V. Tjellesen was founded. In the time leading up to the Second World War and during this time, business was of course complicated by currency and import restrictions, and it was very difficult to make deliveries to the pharmacies. In Copenhagen, the articles were delivered by 10-12 bicycle delivery boys. In the beginning, the wholesale company mainly catered for Copenhagen and Zealand, but due to the excellent performance of Paul Tjellesen, more and more customers emerged in the rest of the country. In 1954, the company moved to Niels Ebbesens Vej 29 in Frederiksberg given the need for larger facilities. In 1963, an increased space requirement, once again, forced the company to buy one of the neighbouring buildings on Niels Ebbesens Vej and H.C. Orsteds Vej. For many years after, H.C. Orsteds Vej 22 was the official address of the company. After more than 45 years in the company, Paul Tjellesen agreed with his son-in-law Peter Sch

  17. Solution of K-V envelope equations

    International Nuclear Information System (INIS)

    Anderson, O.A.

    1995-04-01

    The envelope equations for a KV beam with space charge have been analyzed systematically by an e expansion followed by integrations. The focusing profile as a function of axial length is assumed to be symmetric but otherwise arbitrary. Given the bean current, emittance, and peak focusing field, we find the envelopes a(s) and b(s) and obtain , a max , σ, and σ 0 . Explicit results are presented for various truncations of the expansion. The zeroth order results correspond to those from the well-known smooth approximation; the same convenient format is retained for the higher order cases. The first order results, involving single correction terms, give 3--10 times better accuracy and are good to ∼1% at σ 0 = 70 degree. Third order gives a factor of 10--30 improvement over the smooth approximation and derived quantities accurate to ∼1% at σ 0 = 112 degree. The first order expressions are convenient design tools. They lend themselves to variable energy problems and have been applied to the design, construction, and testing of ESQ accelerators at LBL

  18. A Measurement of the muon neutrino charged current quasielastic interaction and a test of Lorentz violation with the MiniBooNE experiment

    Energy Technology Data Exchange (ETDEWEB)

    Katori, Teppei [Indiana Univ., Bloomington, IN (United States)

    2008-12-01

    The Mini-Booster neutrino experiment (MiniBooNE) at Fermi National Accelerator Laboratory (Fermilab) is designed to search for vμ → ve appearance neutrino oscillations. Muon neutrino charged-current quasi-elastic (CCQE) interactions (vμ + n → μ + p) make up roughly 40% of our data sample, and it is used to constrain the background and cross sections for the oscillation analysis. Using high-statistics MiniBooNE CCQE data, the muon-neutrino CCQE cross section is measured. The nuclear model is tuned precisely using the MiniBooNE data. The measured total cross section is σ = (1.058 ± 0.003 (stat) ± 0.111 (syst)) x 10-38 cm2 at the MiniBooNE muon neutrino beam energy (700-800 MeV). ve appearance candidate data is also used to search for Lorentz violation. Lorentz symmetry is one of the most fundamental symmetries in modern physics. Neutrino oscillations offer a new method to test it. We found that the MiniBooNE result is not well-described using Lorentz violation, however further investigation is required for a more conclusive result.

  19. 1-concave basis for TU games and the library game

    NARCIS (Netherlands)

    Driessen, Theo; Khmelnitskaya, Anna Borisovna; Sales, J.

    2012-01-01

    The study of 1-convex/1-concave TU games possessing a nonempty core and for which the nucleolus is linear was initiated by Driessen and Tijs (Methods Oper. Res. 46:395–406, 1983) and Driessen (OR Spectrum 7:19–26, 1985). However, until recently appealing abstract and practical examples of these

  20. Evaluating the TU/e Lupo EL BEV performance

    NARCIS (Netherlands)

    Besselink, I.J.M.; Wang, J.; Nijmeijer, H.

    2013-01-01

    The TU/e has developed a battery electric vehicle (BEV) using a VW Lupo 3L as donor platform. The differences between the initial design calculations and actual vehicle performance are analysed. Battery charging and discharging efficiency, acceleration performance and top speed are as expected. The

  1. Whole building energy performance anomaly detection at TU/e

    NARCIS (Netherlands)

    Hensen, J.L.M.; Bynum, J.D.

    2013-01-01

    Existing buildings account for the majority of energy consumption in the building sector. Surveys of existing buildings have found an estimated 10-20% reduction in energy consumption may be feasible. Research at the Eindhoven University of Technology (TU/e) is seeking to realize this potential in

  2. A note on the nucleolus for 2-convex TU games

    NARCIS (Netherlands)

    Driessen, Theo; Hou, D.

    For 2-convex n-person cooperative TU games, the nucleolus is determined as some type of constrained equal award rule. Its proof is based on Maschler, Peleg, and Shapley’s geometrical characterization for the intersection of the prekernel with the core. Pairwise bargaining ranges within the core are

  3. Defining a Pedagogical Model: The TU Delft Online Learning Experience

    NARCIS (Netherlands)

    Ribeiro Jorge, N.; Dopper, S.M.; van Valkenburg, W.F.

    2015-01-01

    In early 2014, the Delft University of Technology (TU Delft) started an innovation program with the aim to respond even more effectively to recent developments in open and online education. Drawing on the fields of Distance Education research and the university’s vision of the “engineer of the

  4. First 735 kV transmission, Manicouagan, Montreal

    Energy Technology Data Exchange (ETDEWEB)

    Cahill, L

    1964-11-01

    The 735 kV transmission network of Hydro Quebec is described giving reasons for choice of voltage, stability factors level of insulation, disposition of conductors, insulators, equipment used and state of readiness of the project.

  5. APPLICATION OF SSSC TO THE 330kV NIGERIAN ...

    African Journals Online (AJOL)

    HOD

    2,3 DEPARTMENT OF ELECTRONIC AND ELECTRICAL ENGR., FEDERAL POLYTECHNIC EDE, ... Longitudinal power systems of Nigerian 330 kV transmission network have steady-state ..... C tr ller ” Unpublish Master Thesis Submitted to.

  6. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants

    Directory of Open Access Journals (Sweden)

    Jianming Fu

    2012-01-01

    Full Text Available EF-Tu proteins of plastids, mitochondria, and the cytosolic counterpart EF-1α in plants, as well as EF-Tu proteins of bacteria, are highly conserved and multifunctional. The functions of EF-Tu include transporting the aminoacyl-tRNA complex to the A site of the ribosome during protein biosynthesis; chaperone activity in protecting other proteins from aggregation caused by environmental stresses, facilitating renaturation of proteins when conditions return to normal; displaying a protein disulfide isomerase activity; participating in the degradation of N-terminally blocked proteins by the proteasome; eliciting innate immunity and triggering resistance to pathogenic bacteria in plants; participating in transcription when an E. coli host is infected with phages. EF-Tu genes are upregulated by abiotic stresses in plants, and EF-Tu plays important role in stress responses. Expression of a plant EF-Tu gene confers heat tolerance in E. coli, maize knock-out EF-Tu null mutants are heat susceptible, and over-expression of an EF-Tu gene improves heat tolerance in crop plants. This review paper summarizes the current knowledge of EF-Tu proteins in stress responses in plants and progress on application of EF-Tu for developing crop varieties tolerant to abiotic stresses, such as high temperatures.

  7. Differential expression of the Kv1 voltage-gated potassium channel family in the rat nephron.

    Science.gov (United States)

    Carrisoza-Gaytán, Rolando; Salvador, Carolina; Diaz-Bello, Beatriz; Escobar, Laura I

    2014-10-01

    Several potassium (K(+)) channels contribute to maintaining the resting membrane potential of renal epithelial cells. Apart from buffering the cell membrane potential and cell volume, K(+) channels allow sodium reabsorption in the proximal tubule (PT), K(+) recycling and K(+) reabsorption in the thick ascending limb (TAL) and K(+) secretion and K(+) reabsorption in the distal convoluted tubule (DCT), connecting tubule (CNT) and collecting duct. Previously, we identified Kv.1.1, Kv1.3 and Kv1.6 channels in collecting ducts of the rat inner medulla. We also detected intracellular Kv1.3 channel in the acid secretory intercalated cells, which is trafficked to the apical membrane in response to dietary K(+) to function as a secretory K(+) channel. In this work we sought to characterize the expression of all members of the Kv1 family in the rat nephron. mRNA and protein expression were detected for all Kv1 channels. Immunoblots identified differential expression of each Kv1 in the cortex, outer and inner medulla. Immunofluorescence labeling detected Kv1.5 in Bowman´s capsule and endothelial cells and Kv1.7 in podocytes, endothelial cells and macula densa in glomeruli; Kv1.4, Kv1.5 and Kv1.7 in PT; Kv1.2, Kv1.4 and Kv1.6 in TAL; Kv1.1, Kv1.4 and Kv1.6 in DCT and CNT and Kv1.3 in DCT, and all the Kv1 family in the cortical and medullary collecting ducts. Recently, some hereditary renal syndromes have been attributed to mutations in K(+) channels. Our results expand the repertoire of K(+) channels that contribute to K(+) homeostasis to include the Kv1 family.

  8. Inactivation as a new regulatory mechanism for neuronal Kv7 channels

    DEFF Research Database (Denmark)

    Jensen, Henrik Sindal; Grunnet, Morten; Olesen, Søren-Peter

    2007-01-01

    neuronal channels and are important for controlling excitability. Kv7.1 channels have been considered the only Kv7 channels to undergo inactivation upon depolarization. However, here we demonstrate that inactivation is also an intrinsic property of Kv7.4 and Kv7.5 channels, which inactivate to a larger...

  9. The acrylamide (S)-1 differentially affects Kv7 (KCNQ) potassium channels

    DEFF Research Database (Denmark)

    Bentzen, Bo Hjorth; Schmitt, Nicole; Calloe, Kirstine

    2006-01-01

    The family of Kv7 (KCNQ) potassium channels consists of five members. Kv7.2 and 3 are the primary molecular correlates of the M-current, but also Kv7.4 and Kv7.5 display M-current characteristics. M-channel modulators include blockers (e.g., linopirdine) for cognition enhancement and openers (e.g...

  10. Development of 66 kV/6.9 kV 2 MV A prototype HTS power transformer

    International Nuclear Information System (INIS)

    Bohno, T.; Tomioka, A.; Imaizumi, M.; Sanuki, Y.; Yamamoto, T.; Yasukawa, Y.; Ono, H.; Yagi, Y.; Iwadate, K.

    2005-01-01

    We have developed the technology of the producing a HTS magnet for the power transformer. Three subjects have been mainly studied, high voltage technologies, large current and low AC loss technologies and sub-cooling system technologies to establish the technology of 66 kV/6.9 kV 10 MV A class HTS power transformer. In order to verify the validity of elemental technologies, such as high voltage technologies, large current and low AC loss technologies and sub-cooling system technologies, single-phase 2 MV A class 66 kV/6.9 kV prototype HTS transformer was manufactured and tested. In the load loss (AC loss) measurement, it was obtained that the measured value of 633 W was almost corresponding to the calculated value of 576 W at the rated operation of 2 MV A. Moreover, the breakdown was not found all voltage withstand test. These test results indicate that elemental technologies were established for the development of 66 kV/6.9 kV 10 MV A class HTS power transformer

  11. The Effect of Starspots on Accurate Radius Determination of the Low-Mass Double-Lined Eclipsing Binary Gu Boo

    Science.gov (United States)

    Windmiller, G.; Orosz, J. A.; Etzel, P. B.

    2010-04-01

    GU Boo is one of only a relatively small number of well-studied double-lined eclipsing binaries that contain low-mass stars. López-Morales & Ribas present a comprehensive analysis of multi-color light and radial velocity curves for this system. The GU Boo light curves presented by López-Morales & Ribas had substantial asymmetries, which were attributed to large spots. In spite of the asymmetry, López-Morales & Ribas derived masses and radii accurate to sime2%. We obtained additional photometry of GU Boo using both a CCD and a single-channel photometer and modeled the light curves with the ELC software to determine if the large spots in the light curves give rise to systematic errors at the few percent level. We also modeled the original light curves from the work of López-Morales & Ribas using models with and without spots. We derived a radius of the primary of 0.6329 ± 0.0026 R sun, 0.6413 ± 0.0049 R sun, and 0.6373 ± 0.0029 R sun from the CCD, photoelectric, and López-Morales & Ribas data, respectively. Each of these measurements agrees with the value reported by López-Morales & Ribas (R 1 = 0.623 ± 0.016 R sun) at the level of ≈2%. In addition, the spread in these values is ≈1%-2% from the mean. For the secondary, we derive radii of 0.6074 ± 0.0035 R sun, 0.5944 ± 0.0069 R sun, and 0.5976 ± 0.0059 R sun from the three respective data sets. The López-Morales & Ribas value is R 2 = 0.620 ± 0.020 R sun, which is ≈2%-3% larger than each of the three values we found. The spread in these values is ≈2% from the mean. The systematic difference between our three determinations of the secondary radius and that of López-Morales & Ribas might be attributed to differences in the modeling process and codes used. Our own fits suggest that, for GU Boo at least, using accurate spot modeling of a single set of multi-color light curves results in radii determinations accurate at the ≈2% level.

  12. THE EFFECT OF STARSPOTS ON ACCURATE RADIUS DETERMINATION OF THE LOW-MASS DOUBLE-LINED ECLIPSING BINARY GU Boo

    International Nuclear Information System (INIS)

    Windmiller, G.; Orosz, J. A.; Etzel, P. B.

    2010-01-01

    GU Boo is one of only a relatively small number of well-studied double-lined eclipsing binaries that contain low-mass stars. Lopez-Morales and Ribas present a comprehensive analysis of multi-color light and radial velocity curves for this system. The GU Boo light curves presented by Lopez-Morales and Ribas had substantial asymmetries, which were attributed to large spots. In spite of the asymmetry, Lopez-Morales and Ribas derived masses and radii accurate to ≅2%. We obtained additional photometry of GU Boo using both a CCD and a single-channel photometer and modeled the light curves with the ELC software to determine if the large spots in the light curves give rise to systematic errors at the few percent level. We also modeled the original light curves from the work of Lopez-Morales and Ribas using models with and without spots. We derived a radius of the primary of 0.6329 ± 0.0026 R sun , 0.6413 ± 0.0049 R sun , and 0.6373 ± 0.0029 R sun from the CCD, photoelectric, and Lopez-Morales and Ribas data, respectively. Each of these measurements agrees with the value reported by Lopez-Morales and Ribas (R 1 = 0.623 ± 0.016 R sun ) at the level of ∼2%. In addition, the spread in these values is ∼1%-2% from the mean. For the secondary, we derive radii of 0.6074 ± 0.0035 R sun , 0.5944 ± 0.0069 R sun , and 0.5976 ± 0.0059 R sun from the three respective data sets. The Lopez-Morales and Ribas value is R 2 = 0.620 ± 0.020 R sun , which is ∼2%-3% larger than each of the three values we found. The spread in these values is ∼2% from the mean. The systematic difference between our three determinations of the secondary radius and that of Lopez-Morales and Ribas might be attributed to differences in the modeling process and codes used. Our own fits suggest that, for GU Boo at least, using accurate spot modeling of a single set of multi-color light curves results in radii determinations accurate at the ∼2% level.

  13. VizieR Online Data Catalog: UY UMa and EF Boo compiled time of minima (Yu+, 2017)

    Science.gov (United States)

    Yu, Y.-X.; Zhang, X.-D.; Hu, K.; Xiang, F.-Y.

    2017-11-01

    In order to construct the (O-C) diagram to analyze the period change of UY UMa, we have performed a careful search for all available times of light minima. A total of 76 times of light minima were collected and listed in Table 2. >From the literatures and two well-known databases (i.e., the O-C gateway (http://var.astro.cz/ocgate) and the Lichtenknecker database of the BAV (http://www.bav-astro.de/LkDB/index.php)), we have collected a total of 75 available times of light minima for EF Boo, which are summarized in Table 3. (3 data files).

  14. Major Refit for CERN's 400 kV Substation

    CERN Multimedia

    2001-01-01

    The 400 kV substation on the Prévessin site brings in the electricity that powers CERN's accelerators and the majority of the Laboratory's installations. It was originally built in the 1970s for the SPS, and is one of only five privately owned 400 kV sub-stations in France. Three of the others belong to the national railway company, SNCF, supplying the Paris-Marseilles TGV line, the other is at the Cadarache research centre near mouth of the Rhone. After nearly thirty years of service, CERN's substation has just undergone a complete overhaul. The new main 18 kV switchboard for the SPS pulsed network. The electricity supply for the original Prévessin substation was from the 400 kV EDF network, delivered through three 90 MW transformers at 18 kV to the SPS pulsed network, With the arrival of LEP, two 110 MW transformers were added to supply the new accelerator. Now, as CERN gears up for the LHC, additional pulsed power capacity is needed to supply the transfer lines carrying protons from...

  15. Spectral Irradiance Calibration in the Infrared 11: Comparison of (alpha) Boo and 1 Ceres with a Laboratory Standard

    Science.gov (United States)

    Witteborn, Fred C.; Cohen, Martin; Bregman, Jess D.; Wooden, Diane; Heere, Karen; Shirley, Eric L.

    1998-01-01

    Infrared spectra of two celestial objects frequently used as flux standards are calibrated against an absolute laboratory flux standard at a spectral resolving power of 100 to 200. The spectrum of the K1.5III star, alpha Boo, is measured from 3 microns to 30 microns and that of the C-type asteroid, 1 Ceres, from 5 microns to 30 microns. While these 'standard' spectra do not have the apparent precision of those based on calculated models, they do not require the assumptions involved in theoretical models of stars and asteroids. Specifically they provide a model-independent means of calibrating celestial flux in the spectral range from 12 microns to 30 microns where accurate absolute photometry is not available. The agreement found between the spectral shapes of alpha Boo and Ceres based on laboratory standards, and those based on observed ratios to alpha CMa (Sirius) and alpha Lyr (Vega), flux calibrated by theoretical modeling of these hot stars strengthens our confidence in the applicability of the stellar models as primary irradiance standards.

  16. HARP targets pion production cross section and yield measurements. Implications for MiniBooNE neutrino flux

    Energy Technology Data Exchange (ETDEWEB)

    Wickremasinghe, Don Athula Abeyarathna [Univ. of Cincinnati, OH (United States)

    2015-07-01

    The prediction of the muon neutrino flux from a 71.0 cm long beryllium target for the MiniBooNE experiment is based on a measured pion production cross section which was taken from a short beryllium target (2.0 cm thick - 5% nuclear interaction length) in the Hadron Production (HARP) experiment at CERN. To verify the extrapolation to our longer target, HARP also measured the pion production from 20.0 cm and 40.0 cm beryllium targets. The measured production yields, d2Nπ± (p; θ )=dpd Ω, on targets of 50% and 100% nuclear interaction lengths in the kinematic rage of momentum from 0.75 GeV/c to 6.5 GeV/c and the range of angle from 30 mrad to 210 mrad are presented along with an update of the short target cross sections. The best fitted extended Sanford-Wang (SW) model parameterization for updated short beryllium target π+ production cross section is presented. Yield measurements for all three targets are also compared with that from the Monte Carlo predictions in the MiniBooNE experiment for different SW parameterization. The comparisons of vμ flux predictions for updated SW model is presented.

  17. Molecular Identification and Genetic Diversity of Acropora hyacinthus from Boo and Deer Island, Raja Ampat, West Papua

    Science.gov (United States)

    Wijayanti, DP; Indrayanti, E.; Nuryadi, H.; Dewi, RA; Sabdono, A.

    2018-02-01

    Indonesia lies at the centre of biodiversity for corals. However, the reefs suffered from extensive human exploitation. Marine Protected Areas is thought to be best solution to protect coral reefs ecosystem. Understanding genetic diversity is crucial for effective management of the MPAs, however genetic diversity is rarely been corporate in designing an MPA. Moreover, many MPAs are uneffectively manage due to poor designated and demarcated.Raja Ampat which is located in western tip of West Papua, was designated as a park to mitigatethreatsand protect the valuable marine resources.Scleractinian corals in the genus Acropora are among the most dominant distributed in Raja Ampat waters, including the species of Acroporahyacinthus. The research aimed to analyze genetic diversity and to describe the kinship relationship of Acroporahyacinthus between 2 populations: Boo Island and Deer Island, Raja Ampat. Genetic marker Cytochrome Oxidase I (CO I) of the mitochondrial genome DNA (mtDNA) was used to analyze genetic diversity. Reconstruction of phylogenetic tree and genetic diversity were made by usingsoftware MEGA 5.05 (Moleculer Evolutionary Genetics Analysis). The results of this research indicatecorals A. hyacinthus from Boo Island and Deer Island Raja Ampat are in the low category of genetic diversity and overall had a close genetic relationship of kinship. This is likely due to the small size of the population and few numbers of samples that may not represent the population.

  18. Trust Management in P2P systems using Standard TuLiP

    OpenAIRE

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential storage by providing a sound and complete Lookup and Inference AlgoRithm (LIAR). In this paper we focus on (a) the language of Standard TuLiP and (b) on the practical considerations which arise when d...

  19. HVDC transmission preferred to 750 kV ac

    Energy Technology Data Exchange (ETDEWEB)

    1965-06-25

    It is unlikely that there will be a need in Britain for ac transmission voltages above 400 kV. But with the growing load density in the large conurbations with no possibility of local generation, high voltage dc transmission is likely to be most useful. It was concluded that by 1971 the 400 kV supergrid would be nation-wide and 6,200 circuit miles should be in service. With the expansion to accommodate the large new generating stations, the 400 kV supergrid would become an extremely high power distribution network rather than a transmission system. A higher voltage for transmission is outside the rational limit of speculation for a country the size of Britain.

  20. Skeletal muscle Kv7 (KCNQ) channels in myoblast differentiation and proliferation

    International Nuclear Information System (INIS)

    Roura-Ferrer, Meritxell; Sole, Laura; Martinez-Marmol, Ramon; Villalonga, Nuria; Felipe, Antonio

    2008-01-01

    Voltage-dependent K + channels (Kv) are involved in myocyte proliferation and differentiation by triggering changes in membrane potential and regulating cell volume. Since Kv7 channels may participate in these events, the purpose of this study was to investigate whether skeletal muscle Kv7.1 and Kv7.5 were involved during proliferation and myogenesis. Here we report that, while myotube formation did not regulate Kv7 channels, Kv7.5 was up-regulated during cell cycle progression. Although, Kv7.1 mRNA also increased during the G 1 -phase, pharmacological evidence mainly involves Kv7.5 in myoblast growth. Our results indicate that the cell cycle-dependent expression of Kv7.5 is involved in skeletal muscle cell proliferation

  1. Ionization Electron Signal Processing in Single Phase LArTPCs II. Data/Simulation Comparison and Performance in MicroBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Adams, C.; et al.

    2018-04-07

    The single-phase liquid argon time projection chamber (LArTPC) provides a large amount of detailed information in the form of fine-grained drifted ionization charge from particle traces. To fully utilize this information, the deposited charge must be accurately extracted from the raw digitized waveforms via a robust signal processing chain. Enabled by the ultra-low noise levels associated with cryogenic electronics in the MicroBooNE detector, the precise extraction of ionization charge from the induction wire planes in a single-phase LArTPC is qualitatively demonstrated on MicroBooNE data with event display images, and quantitatively demonstrated via waveform-level and track-level metrics. Improved performance of induction plane calorimetry is demonstrated through the agreement of extracted ionization charge measurements across different wire planes for various event topologies. In addition to the comprehensive waveform-level comparison of data and simulation, a calibration of the cryogenic electronics response is presented and solutions to various MicroBooNE-specific TPC issues are discussed. This work presents an important improvement in LArTPC signal processing, the foundation of reconstruction and therefore physics analyses in MicroBooNE.

  2. Compact 250-kV injector system for PIGMI

    International Nuclear Information System (INIS)

    Hamm, R.W.; Stevens, R.R. Jr.; Mueller, D.W.; Lederer, H.M.

    1978-01-01

    A 250-kV proton injector to be used in the development of a linac suitable for medical applications has been constructed. This injector utilizes a spherical Pierce geometry to produce a converging beam. A gas insulated accelerating column is cantilevered on a grounded vacuum system, with a separate high voltage equipment dome connected to a 300-kV Cockcroft-Walton power supply. The injector can be operated locally or remotely, with the remote control accomplished by a microprocessor system linked to a central control minicomputer. This injector has been designed as a low-cost compact system. The design details and the data obtained during initial operation are presented

  3. Mutation of the conserved Gly83 and Gly94 in Escherichia coli elongation factor Tu. Indication of structural pivots

    DEFF Research Database (Denmark)

    Kjaersgård, I V; Knudsen, Charlotte Rohde; Wiborg, O

    1995-01-01

    Elongation factor Tu from Escherichia coli cycles between an active conformation where GTP is bound, and an inactive conformation where GDP is bound. Between the two conformations, elongation factor Tu undergoes major structural changes. The aim of this work has been to reveal the role of two ver...... is an important pivot point in elongation factor-Tu. Udgivelsesdato: 1995-Feb-15...

  4. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-11, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    Dette notat dokumenterer de leverede data i datasættet TU 2006-11 version 1, som omfatter data fra perioden maj 2006 til 31. december 2011. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger såvidt muligt datasættet og opdateres ikke...

  5. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-12, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    Dette notat dokumenterer de leverede data i datasættet TU 2006-12 version 1, som omfatter data fra perioden maj 2006 til 31. december 2012. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger så vidt muligt datasættet og opdateres ikke...

  6. Transportvaneundersøgelsen - Variabeldeklaration: TU 2006-13 version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    Dette notat dokumenterer de leverede data i datasættet TU 2006-13 version 2, som omfatter data fra perioden maj 2006 til 30. april 2014. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger så vidt muligt datasættet og opdateres ikke...

  7. The Danish National Travel Survey - declaration of variables : TU 2006-10, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Haunstrup, Brian

    This record documents the reported data in the data set TU 2006-10 version 1 covering data from the period May 2006 until 30 April 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  8. The Danish National Travel Survey - declaration of variables : TU 2006-13, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-13 version 2 covering data from the period May 2006 until 30. April 2014. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  9. Transportvaneundersøgelsen - Variabeldeklaration: TU 2006-14 version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    Dette notat dokumenterer de leverede data i datasættet TU 2006-14 version 1, som omfatter data fra perioden maj 2006 til 31. december 2014. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger så vidt muligt datasættet og opdateres ikke...

  10. Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis

    DEFF Research Database (Denmark)

    Villa, Elizabeth; Sengupta, Jayati; Trabuco, Leonard G.

    2009-01-01

    In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.......7-A cryo-electron microscopy map of the aminoacyl-tRNA x EF-Tu x GDP x kirromycin-bound Escherichia coli ribosome, together with an atomic model of the complex obtained through molecular dynamics flexible fitting. The model reveals the conformational changes in the conserved GTPase switch regions...... of EF-Tu that trigger hydrolysis of GTP, along with key interactions, including those between the sarcin-ricin loop and the P loop of EF-Tu, and between the effector loop of EF-Tu and a conserved region of the 16S rRNA. Our data suggest that GTP hydrolysis on EF-Tu is controlled through a hydrophobic...

  11. The Danish National Travel Survey - declaration of variables : TU 2006-12, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 31. December 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  12. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-10, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    Dette notat dokumenterer de leverede data i datasættet TU 2006-10 version 1, som omfatter data fra perioden maj 2006 til 31. december 2010. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger såvidt muligt datasættet og opdateres ikke...

  13. The Danish National Travel Survey - declaration of variables TU 2006-12, version 2 : Documentation note

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 3o April 2013. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  14. The Danish National Travel Survey - declaration of variables TU 2006-11, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 1 covering data from the period May 2006 until 31 December 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  15. The Danish National Travel Survey - declaration of variables TU 2006-11, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 2 covering data from the period May 2006 until 30. April 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  16. Kv1 channels and neural processing in vestibular calyx afferents

    Directory of Open Access Journals (Sweden)

    Frances L Meredith

    2015-06-01

    Full Text Available Potassium-selective ion channels are important for accurate transmission of signals from auditory and vestibular sensory end organs to their targets in the central nervous system. During different gravity conditions, astronauts experience altered input signals from the peripheral vestibular system resulting in sensorimotor dysfunction. Adaptation to altered sensory input occurs, but it is not explicitly known whether this involves synaptic modifications within the vestibular epithelia. Future investigations of such potential plasticity require a better understanding of the electrophysiological mechanisms underlying the known heterogeneity of afferent discharge under normal conditions. This study advances this understanding by examining the role of the Kv1 potassium channel family in mediating action potentials in specialized vestibular afferent calyx endings in the gerbil crista and utricle. Pharmacological agents selective for different sub-types of Kv1 channels were tested on membrane responses in whole cell recordings in the crista. Kv1 channels sensitive to α-dendrotoxin and dendrotoxin-K were found to prevail in the central regions, whereas K+ channels sensitive to margatoxin, which blocks Kv1.3 and 1.6 channels, were more prominent in peripheral regions. Margatoxin-sensitive currents showed voltage-dependent inactivation. Dendrotoxin-sensitive currents showed no inactivation and dampened excitability in calyces in central neuroepithelial regions. The differential distribution of Kv1 potassium channels in vestibular afferents supports their importance in accurately relaying gravitational and head movement signals through specialized lines to the central nervous system. Pharmacological modulation of specific groups of K+ channels could help alleviate vestibular dysfunction on earth and in space.

  17. Kv1 channels and neural processing in vestibular calyx afferents.

    Science.gov (United States)

    Meredith, Frances L; Kirk, Matthew E; Rennie, Katherine J

    2015-01-01

    Potassium-selective ion channels are important for accurate transmission of signals from auditory and vestibular sensory end organs to their targets in the central nervous system. During different gravity conditions, astronauts experience altered input signals from the peripheral vestibular system resulting in sensorimotor dysfunction. Adaptation to altered sensory input occurs, but it is not explicitly known whether this involves synaptic modifications within the vestibular epithelia. Future investigations of such potential plasticity require a better understanding of the electrophysiological mechanisms underlying the known heterogeneity of afferent discharge under normal conditions. This study advances this understanding by examining the role of the Kv1 potassium channel family in mediating action potentials in specialized vestibular afferent calyx endings in the gerbil crista and utricle. Pharmacological agents selective for different sub-types of Kv1 channels were tested on membrane responses in whole cell recordings in the crista. Kv1 channels sensitive to α-dendrotoxin and dendrotoxin-K were found to prevail in the central regions, whereas K(+) channels sensitive to margatoxin, which blocks Kv1.3 and 1.6 channels, were more prominent in peripheral regions. Margatoxin-sensitive currents showed voltage-dependent inactivation. Dendrotoxin-sensitive currents showed no inactivation and dampened excitability in calyces in central neuroepithelial regions. The differential distribution of Kv1 potassium channels in vestibular afferents supports their importance in accurately relaying gravitational and head movement signals through specialized lines to the central nervous system. Pharmacological modulation of specific groups of K(+) channels could help alleviate vestibular dysfunction on earth and in space.

  18. The role of Glu259 in Escherichia coli elongation factor Tu in ternary complex formation

    DEFF Research Database (Denmark)

    Nautrup Pedersen, Gitte; Rattenborg, Thomas; Knudsen, Charlotte Rohde

    1998-01-01

    Determination of the crystal structure of the ternary complex formed between elongation factor Tu:GTP and aminoacylated tRNA revealed three regions of interaction between elongation factor Tu and tRNA. The structure indicates that the conserved glutamic acid at position 271 in Thermus aquaticus EF-Tu...... could be involved in the binding of the 3' CCA-Phe end of the aminoacylated tRNA. Therefore, the corresponding residue, Glu259, of Escherichia coli EF-Tu was mutated into alanine, aspartic acid, glutamine and tyrosine, in order to substantiate the crystallographic structural evidence and to obtain...... of interaction with tRNA, while mutation to tyrosine abolished completely the interaction with tRNA. Finally, mutation to glutamine resulted in an elongation factor Tu variant behaving like the wild type. In conclusion, the environment around the site binding the CCA-Phe end of the tRNA is very restricted...

  19. Event Excess in the MiniBooNE Search for νμ→νe Oscillations

    International Nuclear Information System (INIS)

    Aguilar-Arevalo, A. A.; Anderson, C. E.; Fleming, B. T.; Linden, S. K.; Spitz, J.; Brice, S. J.; Brown, B. C.; Ford, R.; Garcia, F. G.; Kobilarcik, T.; Marsh, W.; Moore, C. D.; Polly, C. C.; Russell, A. D.; Stefanski, R. J.; Zeller, G. P.; Bugel, L.; Conrad, J. M.; Karagiorgi, G.; Nguyen, V.

    2010-01-01

    The MiniBooNE experiment at Fermilab reports results from a search for ν μ →ν e oscillations, using a data sample corresponding to 5.66x10 20 protons on target. An excess of 20.9±14.0 events is observed in the energy range 475 ν QE μ events, has a probability for consistency with the background-only hypothesis of 0.5%. On the other hand, fitting for ν μ →ν e oscillations, the best-fit point has a χ 2 probability of 8.7%. The data are consistent with ν μ →ν e oscillations in the 0.1 to 1.0 eV 2 Δm 2 range and with the evidence for antineutrino oscillations from the Liquid Scintillator Neutrino Detector at Los Alamos National Laboratory.

  20. Survey of magnetic fields near BPA 230-kV and 500-kV transmission lines

    International Nuclear Information System (INIS)

    Perrin, N.; Aggarwal, R.P.

    1991-01-01

    The purpose of this study was to characterize typical levels and variability of 60Hz magnetic fields at the centerline and edge of right-of-way of Bonneville Power Administration (BPA) 230-kV and 500-kV transmission lines. This was accomplished by taking magnetic field measurements at over 800 spans in Oregon and Washington. The spans were sampled using a stratified random sampling procedure with region (East vs. West), voltage (230-kV vs 500-kV), and circuit configuration as strata. There were five different circuit configuration groups for each region/voltage category requiring a total of 200 strata. Magnetic field measurements were taken at 13 locations under each span using an EMDEX-C as a survey meter. Additional information recorded for each span included conductor height (at 10 locations), right-of-way width, longitudinal and lateral slope, time of day, vegetation, terrain, weather conditions, temperature, wind speed, span length and presence of other lines in the corridor. 9 refs., 17 figs., 26 tabs

  1. KCNE4 is an inhibitory subunit to Kv1.1 and Kv1.3 potassium channels

    DEFF Research Database (Denmark)

    Grunnet, Morten; Rasmussen, Hannne B; Hay-Schmidt, Anders

    2003-01-01

    is detected in the heart and in five different parts of the brain. Having the broad distribution of Kv1 channels in mind, the demonstrated inhibitory property of KCNE4-subunits could locally and/or transiently have a dramatic influence on cellular excitability and on setting resting membrane potentials....

  2. The gammaTuRC Nanomachine Mechanism and Future Applications

    Science.gov (United States)

    Riehlman, Timothy D.

    The complexity and precision of the eukaryotic cell's cytoskeletal network is unrivaled by any man-made systems, perfected by billions of years of evolution, mastering elegant processes of self-assembly, error correction, and self-repair. Understanding the capabilities of these networks will have important and far reaching applications in human medicine by aiding our understanding of developmental processes, cellular division, and disease mechanisms, and through biomimicry will provide insights for biosynthetic manufacturing at the nanoscale and across scales. My research utilizes cross species techniques from Human to the model organism of Fission Yeast to investigate the structure and mechanisms of the g-tubulin ring complex (gTuRC). The gTuRC is a highly conserved eukaryotic multiprotein complex serving as a microtubule organizing center (MTOC) responsible for microtubule nucleation through templating, regulation of dynamics, and establishment of microtubule polarity. Microtubules are 25 nm diameter dynamic flexible polymers of a/b-tubulin heterodimers that function as scaffolds, force generators, distributors, and intracellular highways. The microtubule cytoskeleton is essential for numerous fundamental cellular processes such as mitotic division of chromosomes and cell division, organelle distribution within the cell, cell signaling, and cell shape. This incredible diversity in functions is made possible in part due to molecular motor Kinesin-like proteins (Klps), which allow expansion into more specialized neural, immune, and ciliated cell functions. Combined, the MTOC, microtubules, and Klps represent ideal microtubule cytoskeleton protein (MCP) modular components for in vitro biomimicry towards generation of adaptable patterned networks for human designed applications. My research investigates the hypothesis that a mechanistic understanding of conserved MTOC gTuRC mechanisms will help us understand dynamic cellular nanomachines and their ability to self

  3. TU Electric reactor physics model verification: Power reactor benchmark

    International Nuclear Information System (INIS)

    Willingham, C.E.; Killgore, M.R.

    1988-01-01

    Power reactor benchmark calculations using the advanced code package CASMO-3/SIMULATE-3 have been performed for six cycles of Prairie Island Unit 1. The reload fuel designs for the selected cycles included gadolinia as a burnable absorber, natural uranium axial blankets and increased water-to-fuel ratio. The calculated results for both startup reactor physics tests (boron endpoints, control rod worths, and isothermal temperature coefficients) and full power depletion results were compared to measured plant data. These comparisons show that the TU Electric reactor physics models accurately predict important measured parameters for power reactors

  4. The Measure your World / Mide tu Mundo Project

    Science.gov (United States)

    Hojman, S.; Johnson, R. M.; Meymaris, K. K.; Ward, D. L.; Russell, R.; Genyuk, J.; Lagrave, M.; Henderson, S.; Ostrosky, J.; Martinez, M.

    2007-12-01

    Over 22 centuries ago Erathostenes devised a method to determine the Earth radius. Measure your World / Mide tu Mundo is a joint venture lead by Windows to the Universe/Ventanas al Universo in the United States of America, EducaRed in Chile and RedEscolar in Mexico seeking to partner teams of students, teachers and parents from the three countries in a collaborative effort to determine the Earth circumference by sharing the results of their measurements, and exchanging cultural information. Data are collected from September 29 through October 7, 2007. A report of the whole experience will be presented.

  5. 35-kV GaAs subnanosecond photoconductive switches

    Energy Technology Data Exchange (ETDEWEB)

    Pocha, M.D.; Druce, R.L. (Lawrence Livermore National Lab., CA (United States))

    1990-12-01

    Photoconductive switches are one of the few devices that allow the generation of high-voltage electrical pulses with subnanosecond rise time. The authors are exploring high-voltage, fast-pulse generation using GaAs photoconductive switches. They have been able to generate 35-kV pulses with rise times as short as 135 ps using 5-mm gap switches and have achieved electric field hold-off of greater than 100 kV/cm. They have also been able to generate an approximately 500-ps FWHM on/off electrical pulse with an amplitude of approximately 3 kV using neutron-irradiated GaAs having short carrier life times. This paper describes the experimental results and discusses fabrication of switches and the diagnostics used to measure these fast signals. They also describe the experience with the nonlinear lock-on and avalanche modes of operation observed in GaAs.

  6. 35-kV GaAs subnanosecond photoconductive switches

    Science.gov (United States)

    Pocha, Michael D.; Druce, Robert L.

    1990-12-01

    High-voltage, fast-pulse generation using GaAs photoconductive switches is investigated. It is possible to to generate 35-kV pulses with risetimes as short as 135 ps using 5-mm gap switches, and electric field hold-off of greater than 100 kV/cm is achieved. An approximately 500-ps FWHM on/off electrical pulse is generated with an amplitude of approximately 3 kV using neutron-irradiated GaAs having short carrier lifetimes. Experimental results are described, and fabrication of switches and the diagnostics used to measure these fast signals are discussed. Experience with the nonlinear lock-on and avalanche modes of operation observed in GaAs is also described.

  7. CIAE 600 kV ns pulse neutron generator

    International Nuclear Information System (INIS)

    Shen Guanren; Guan Xialing; Chen Hongtao

    2001-01-01

    The overall composition of CIAE 600 kV ns Pulse Neutron Generator (CPNG) are introduced, and its characteristic, main technological performance and application were also given. CPNG consists of high voltage power supply with highest output voltage 600 kV, direct current 15 mA, stability and ripple ≤0.1%, 2214 mm x 1604 mm x 1504 mm stainless steel high voltage electrode, built in head equipment uniform field accelerating tube, ns pulsed installation, turbomolecular vacuum pump system and drift pipes at 0 degree and 45 degree. Its characteristics are: (1) high current beam; (2) high current beam ns pulsed installation made use of low energy for chopper and high energy for buncher; (3) compactly laid out and simple in structure

  8. CEBAF 200 kV Inverted Electron Gun

    International Nuclear Information System (INIS)

    Grames, J.M.; Adderley, P.A.; Clark, J.; Hansknecht, J.; Poelker, M.; Stutzman, M.L.; Suleiman, R.; Surles-Law, K.E.L.

    2011-01-01

    Two DC high voltage GaAs photoguns have been built at Jefferson Lab based on a compact inverted insulator design. One photogun provides the polarized electron beam at CEBAF and operates at 130 kV bias voltage. The other gun is used for high average current lifetime studies at a dedicated test facility and has been operated at bias voltage up to 225 kV. The advantages of higher DC voltage for CEBAF include reduced space-charge emittance growth and the potential for prolonged photocathode lifetime. However, a consequence of operating at higher voltages is the increased likelihood of field emission or breakdown, both of which are unacceptable. Highlights of the R and D studies leading toward a production 200keV GaAs photogun for CEBAF will be presented.

  9. A compact 100 kV high voltage glycol capacitor.

    Science.gov (United States)

    Wang, Langning; Liu, Jinliang; Feng, Jiahuai

    2015-01-01

    A high voltage capacitor is described in this paper. The capacitor uses glycerol as energy storage medium, has a large capacitance close to 1 nF, can hold off voltages of up to 100 kV for μs charging time. Allowing for low inductance, the capacitor electrode is designed as coaxial structure, which is different from the common structure of the ceramic capacitor. With a steady capacitance at different frequencies and a high hold-off voltage of up to 100 kV, the glycol capacitor design provides a potential substitute for the ceramic capacitors in pulse-forming network modulator to generate high voltage pulses with a width longer than 100 ns.

  10. Bladder contractility is modulated by Kv7 channels in pig detrusor

    DEFF Research Database (Denmark)

    Svalø, Julie; Bille, Michala; Parameswaran Theepakaran, Neeraja

    2013-01-01

    Kv7 channels are involved in smooth muscle relaxation, and accordingly we believe that they constitute potential targets for the treatment of overactive bladder syndrome. We have therefore used myography to examine the function of Kv7 channels in detrusor, i.e. pig bladder, with a view...... relaxation, suggesting that Kv7.2 and/or Kv7.4 channels constitute the subtypes that are relevant to bladder contractility. The effects of retigabine and ML213 were attenuated by pre-incubation with 10µM XE991 (Kv7.1-7.5 channel blocker) (P...

  11. ROMANO-WARD SYNDROME ASSOCIATED WITH TU ELECTRICAL ALTERNANS

    Directory of Open Access Journals (Sweden)

    DJAWAN

    1981-05-01

    Full Text Available A case o f the electrical alternans of t he TU wave and peri odic nega t ive U wave associated with c l ini cal symptoms , e lectrocardi ographic a nd postmortem findi ng s o f Romano- Ward Syndrome has been pres ented. No e lec ~ r o l y te d ist urbance was found t o be r esponsible for t his exceptional l y rare situation . Changes i n A-V conduct i on and left bu n• d Ie branch block could be a t tributed to the d i f f use c or onary s clero sis and s ubs equent i schemia in the myocardial c onduction t.issues . The e lectrical alternans of t he U wave or TU complex of the e lectrocardiogram i s an exceeding ly r are s i t uation without any clearly known mechanism for i ts appea rance . A case of thi s phenome no~ i n as soc iation with RomanoWard Syndrome has been presented whe rein an abnorma l ity i n A-V conduction and left bund le branch block cou ld be encountered .

  12. Doc toxin is a kinase that inactivates elongation factor Tu.

    Science.gov (United States)

    Cruz, Jonathan W; Rothenbacher, Francesca P; Maehigashi, Tatsuya; Lane, William S; Dunham, Christine M; Woychik, Nancy A

    2014-03-14

    The Doc toxin from bacteriophage P1 (of the phd-doc toxin-antitoxin system) has served as a model for the family of Doc toxins, many of which are harbored in the genomes of pathogens. We have shown previously that the mode of action of this toxin is distinct from the majority derived from toxin-antitoxin systems: it does not cleave RNA; in fact P1 Doc expression leads to mRNA stabilization. However, the molecular triggers that lead to translation arrest are not understood. The presence of a Fic domain, albeit slightly altered in length and at the catalytic site, provided a clue to the mechanism of P1 Doc action, as most proteins with this conserved domain inactivate GTPases through addition of an adenylyl group (also referred to as AMPylation). We demonstrated that P1 Doc added a single phosphate group to the essential translation elongation factor and GTPase, elongation factor (EF)-Tu. The phosphorylation site was at a highly conserved threonine, Thr-382, which was blocked when EF-Tu was treated with the antibiotic kirromycin. Therefore, we have established that Fic domain proteins can function as kinases. This distinct enzymatic activity exhibited by P1 Doc also solves the mystery of the degenerate Fic motif unique to the Doc family of toxins. Moreover, we have established that all characterized Fic domain proteins, even those that phosphorylate, target pivotal GTPases for inactivation through a post-translational modification at a single functionally critical acceptor site.

  13. Doc Toxin Is a Kinase That Inactivates Elongation Factor Tu*

    Science.gov (United States)

    Cruz, Jonathan W.; Rothenbacher, Francesca P.; Maehigashi, Tatsuya; Lane, William S.; Dunham, Christine M.; Woychik, Nancy A.

    2014-01-01

    The Doc toxin from bacteriophage P1 (of the phd-doc toxin-antitoxin system) has served as a model for the family of Doc toxins, many of which are harbored in the genomes of pathogens. We have shown previously that the mode of action of this toxin is distinct from the majority derived from toxin-antitoxin systems: it does not cleave RNA; in fact P1 Doc expression leads to mRNA stabilization. However, the molecular triggers that lead to translation arrest are not understood. The presence of a Fic domain, albeit slightly altered in length and at the catalytic site, provided a clue to the mechanism of P1 Doc action, as most proteins with this conserved domain inactivate GTPases through addition of an adenylyl group (also referred to as AMPylation). We demonstrated that P1 Doc added a single phosphate group to the essential translation elongation factor and GTPase, elongation factor (EF)-Tu. The phosphorylation site was at a highly conserved threonine, Thr-382, which was blocked when EF-Tu was treated with the antibiotic kirromycin. Therefore, we have established that Fic domain proteins can function as kinases. This distinct enzymatic activity exhibited by P1 Doc also solves the mystery of the degenerate Fic motif unique to the Doc family of toxins. Moreover, we have established that all characterized Fic domain proteins, even those that phosphorylate, target pivotal GTPases for inactivation through a post-translational modification at a single functionally critical acceptor site. PMID:24448800

  14. CONTRIBUTIONS OF INTRACELLULAR IONS TO Kv CHANNEL VOLTAGE SENSOR DYNAMICS.

    Directory of Open Access Journals (Sweden)

    Samuel eGoodchild

    2012-06-01

    Full Text Available Voltage sensing domains of Kv channels control ionic conductance through coupling of the movement of charged residues in the S4 segment to conformational changes at the cytoplasmic region of the pore domain, that allow K+ ions to flow. Conformational transitions within the voltage sensing domain caused by changes in the applied voltage across the membrane field are coupled to the conducting pore region and the gating of ionic conductance. However, several other factors not directly linked to the voltage dependent movement of charged residues within the voltage sensor impact the dynamics of the voltage sensor, such as inactivation, ionic conductance, intracellular ion identity and block of the channel by intracellular ligands. The effect of intracellular ions on voltage sensor dynamics is of importance in the interpretation of gating current measurements and the physiology of pore/voltage sensor coupling. There is a significant amount of variability in the reported kinetics of voltage sensor deactivation kinetics of Kv channels attributed to different mechanisms such as open state stabilization, immobilization and relaxation processes of the voltage sensor. Here we separate these factors and focus on the causal role that intracellular ions can play in allosterically modulating the dynamics of Kv voltage sensor deactivation kinetics. These considerations are of critical importance in understanding the molecular determinants of the complete channel gating cycle from activation to deactivation.

  15. The period analysis of V418 AQL, SU BOO, RV CVn, CR CAS, GV CYG, V432 PER, and BD+42 2782

    International Nuclear Information System (INIS)

    Zasche, P.; Wolf, M.; Kučáková, H.; Uhlař, R.

    2014-01-01

    The minimum timings of eclipsing binaries V418 Aql, SU Boo, RV CVn, CR Cas, GV Cyg, V432 Per, and BD+42 2782 were collected and analyzed. Their long-term behavior was studied via period analysis, revealing a periodic term in eclipse times. We derived 576 new times of minimum. Hence, to describe the periodic variation, a third-body hypothesis was proposed and the resulting orbital periods are as follows: 70, 7.4, 53, 37, 27, 53, and 18 yr, respectively. For the system V432 Per an additional 9.5 yr variation was also found. The predicted minimum masses of these distant bodies were calculated and their detectability discussed. The light curves of SU Boo and RV CVn were analyzed using the PHOEBE program, resulting in physical parameters of the components. New variable stars in the field of V418 Aql were discovered.

  16. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice.

    Science.gov (United States)

    Menegola, Milena; Clark, Eliana; Trimmer, James S

    2012-06-01

    To gain insights into the phenotype of voltage-gated potassium (Kv)1.1 and Kv4.2 knockout mice, we used immunohistochemistry to analyze the expression of component principal or α subunits and auxiliary subunits of neuronal Kv channels in knockout mouse brains. Genetic ablation of the Kv1.1 α subunit did not result in compensatory changes in the expression levels or subcellular distribution of related ion channel subunits in hippocampal medial perforant path and mossy fiber nerve terminals, where high levels of Kv1.1 are normally expressed. Genetic ablation of the Kv4.2 α subunit did not result in altered neuronal cytoarchitecture of the hippocampus. Although Kv4.2 knockout mice did not exhibit compensatory changes in the expression levels or subcellular distribution of the related Kv4.3 α subunit, we found dramatic decreases in the cellular and subcellular expression of specific Kv channel interacting proteins (KChIPs) that reflected their degree of association and colocalization with Kv4.2 in wild-type mouse and rat brains. These studies highlight the insights that can be gained by performing detailed immunohistochemical analyses of Kv channel knockout mouse brains. Wiley Periodicals, Inc. © 2012 International League Against Epilepsy.

  17. Measurement of cosmic-ray reconstruction efficiencies in the MicroBooNE LArTPC using a small external cosmic-ray counter

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; et al.

    2017-07-31

    The MicroBooNE detector is a liquid argon time projection chamber at Fermilab designed to study short-baseline neutrino oscillations and neutrino-argon interaction cross-section. Due to its location near the surface, a good understanding of cosmic muons as a source of backgrounds is of fundamental importance for the experiment. We present a method of using an external 0.5 m (L) x 0.5 m (W) muon counter stack, installed above the main detector, to determine the cosmic-ray reconstruction efficiency in MicroBooNE. Data are acquired with this external muon counter stack placed in three different positions, corresponding to cosmic rays intersecting different parts of the detector. The data reconstruction efficiency of tracks in the detector is found to be $\\epsilon_{\\mathrm{data}}=(97.1\\pm0.1~(\\mathrm{stat}) \\pm 1.4~(\\mathrm{sys}))\\%$, in good agreement with the Monte Carlo reconstruction efficiency $\\epsilon_{\\mathrm{MC}} = (97.4\\pm0.1)\\%$. This analysis represents a small-scale demonstration of the method that can be used with future data coming from a recently installed cosmic-ray tagger system, which will be able to tag $\\approx80\\%$ of the cosmic rays passing through the MicroBooNE detector.

  18. Elemental abundance analyses with DAO spectrograms: XXXII. HR 6455 (A3 III), δ Aqr (A3 V), η Lep (F2 V), and 1 Boo (A1 V)

    Science.gov (United States)

    Yüce, K.; Adelman, S. J.; Gulliver, A. F.; Hill, G.

    2011-08-01

    We examine the sharp-lined stars HR 6455 (A3 III, v sin i = 8.7 km s-1) and η Lep (F2 V, v sin i = 13.5 km s-1) as well as δ Aqr (A3 V, v sin i = 81 km s-1) and 1 Boo (A1 V, v sin i = 59 km s-1) to increase the number consistently analyzed A and F stars using high dispersion and high S/N (≥200) spectrograms obtained with CCD detectors at the long Coudé camera of the 1.22-m telescope of the Dominion Astrophysical Observatory. Such studies contribute to understanding systematic abundance differences between normal and non-magnetic main-sequence band chemically peculiar A and early F stars. LTE fine analyses of HR 6455, δ Aqr, and 1 Boo using Kurucz's ATLAS suite programs show the same general elemental abundance trends with differences in the metal richness. Light and iron-peak element abundances are generally solar or overabundant while heavy element and rare earth element abundances are overabundant. HR 6455 is an evolved Am star while δ Aqr and 1 Boo show the phenomenon to different extents. Most derived abundances of η Lep are solar. Table 3 is available at the CDS via http://cdsarc.u-strasbg.fr/cgi-bin/qcat?J/AN/332/681

  19. Measurement of cosmic-ray reconstruction efficiencies in the MicroBooNE LArTPC using a small external cosmic-ray counter

    Science.gov (United States)

    Acciarri, R.; Adams, C.; An, R.; Anthony, J.; Asaadi, J.; Auger, M.; Bagby, L.; Balasubramanian, S.; Baller, B.; Barnes, C.; Barr, G.; Bass, M.; Bay, F.; Bishai, M.; Blake, A.; Bolton, T.; Camilleri, L.; Caratelli, D.; Carls, B.; Castillo Fernandez, R.; Cavanna, F.; Chen, H.; Church, E.; Cianci, D.; Cohen, E.; Collin, G. H.; Conrad, J. M.; Convery, M.; Crespo-Anadón, J. I.; Del Tutto, M.; Devitt, D.; Dytman, S.; Eberly, B.; Ereditato, A.; Escudero Sanchez, L.; Esquivel, J.; Fadeeva, A. A.; Fleming, B. T.; Foreman, W.; Furmanski, A. P.; Garcia-Gamez, D.; Garvey, G. T.; Genty, V.; Goeldi, D.; Gollapinni, S.; Graf, N.; Gramellini, E.; Greenlee, H.; Grosso, R.; Guenette, R.; Hackenburg, A.; Hamilton, P.; Hen, O.; Hewes, J.; Hill, C.; Ho, J.; Horton-Smith, G.; Hourlier, A.; Huang, E.-C.; James, C.; de Vries, J. Jan; Jen, C.-M.; Jiang, L.; Johnson, R. A.; Joshi, J.; Jostlein, H.; Kaleko, D.; Kalousis, L. N.; Karagiorgi, G.; Ketchum, W.; Kirby, B.; Kirby, M.; Kobilarcik, T.; Kreslo, I.; Lange, G.; Laube, A.; Li, Y.; Lister, A.; Littlejohn, B. R.; Lockwitz, S.; Lorca, D.; Louis, W. C.; Luethi, M.; Lundberg, B.; Luo, X.; Marchionni, A.; Mariani, C.; Marshall, J.; Martinez Caicedo, D. A.; Meddage, V.; Miceli, T.; Mills, G. B.; Moon, J.; Mooney, M.; Moore, C. D.; Mousseau, J.; Murrells, R.; Naples, D.; Nienaber, P.; Nowak, J.; Palamara, O.; Paolone, V.; Papavassiliou, V.; Pate, S. F.; Pavlovic, Z.; Pelkey, R.; Piasetzky, E.; Porzio, D.; Pulliam, G.; Qian, X.; Raaf, J. L.; Rafique, A.; Rochester, L.; von Rohr, C. Rudolf; Russell, B.; Schmitz, D. W.; Schukraft, A.; Seligman, W.; Shaevitz, M. H.; Sinclair, J.; Smith, A.; Snider, E. L.; Soderberg, M.; Söldner-Rembold, S.; Soleti, S. R.; Spentzouris, P.; Spitz, J.; John, J. St.; Strauss, T.; Szelc, A. M.; Tagg, N.; Terao, K.; Thomson, M.; Toups, M.; Tsai, Y.-T.; Tufanli, S.; Usher, T.; Van De Pontseele, W.; Van de Water, R. G.; Viren, B.; Weber, M.; Wickremasinghe, D. A.; Wolbers, S.; Wongjirad, T.; Woodruff, K.; Yang, T.; Yates, L.; Zeller, G. P.; Zennamo, J.; Zhang, C.

    2017-12-01

    The MicroBooNE detector is a liquid argon time projection chamber at Fermilab designed to study short-baseline neutrino oscillations and neutrino-argon interaction cross-section. Due to its location near the surface, a good understanding of cosmic muons as a source of backgrounds is of fundamental importance for the experiment. We present a method of using an external 0.5 m (L) × 0.5 m (W) muon counter stack, installed above the main detector, to determine the cosmic-ray reconstruction efficiency in MicroBooNE. Data are acquired with this external muon counter stack placed in three different positions, corresponding to cosmic rays intersecting different parts of the detector. The data reconstruction efficiency of tracks in the detector is found to be epsilondata=(97.1±0.1 (stat) ± 1.4 (sys))%, in good agreement with the Monte Carlo reconstruction efficiency epsilonMC = (97.4±0.1)%. This analysis represents a small-scale demonstration of the method that can be used with future data coming from a recently installed cosmic-ray tagger system, which will be able to tag ≈80% of the cosmic rays passing through the MicroBooNE detector.

  20. A Decision-Making Model to Attract Private Sector Investors into BOT and BOO Water and Sanitation Projects

    Directory of Open Access Journals (Sweden)

    Elnaz Abbaszadeh

    2017-03-01

    Full Text Available Economic development in any country depends on a multitude of factors, among which infrastructure construction projects constitute a major part. Included in this category are large-scale water supply and sanitation schemes that are commonly state-owned and require large allocations from the public funds. Article 214 of the 5th National Development Plan of the Islamic Republic of Iran mandates the Government to employ such public-private partnership tools as BOT (Build-Operate-Transfer and BOO (Build-Own-Operate as the means towards attracting private sector participation to ensure enhanced the efficiency and effectiveness of infrastructure projects. It is the objective of the present study to survey and identify the challenges facing such schemes based on the assessment of the contractual criteria and conditions commonly practiced in water and sanitation projects. The results are expected to lead to the development of a decision-making model that will encourage the private sector’s decision-makers to invest in these projects. Information was collected in two stages. The first stage involved a review of model contracts and interviews with water and sanitation experts. The data thus obtained was subjected to analysis using the SPSS software, which yielded 14 indices as the major components of the public-private partnership contracts used in the industry. In a second stage, the indices were tabulated and prepared in the form of a questionnaire which was presented to experts who were asked to assign weight scores of 1 to 10 to each of the indices. Ranking and scoring was accomplished using the TOPSIS method. Finally, the Pareto diagram was used to select the 10 more important indices which were further divided into two major and minor categories of indices. A model was then developed for scoring by the private sector investment advisors to help them in their decision-making process regarding BOD and BOO water and sanitation projects. The model

  1. MiniBooNE first results on a search for νe appearance at the Δm2 ∼ 1 eV2 scale

    International Nuclear Information System (INIS)

    Sorel, M.; Columbia U.

    2007-01-01

    Solar and atmospheric neutrino oscillations, recently confirmed by reactor and accelerator-based experiments, are now well established. On the other hand, the interpretation of the LSND (bar ν) e excess [1] as (bar ν) μ → (bar ν) e oscillations at the Δm 2 ∼ 1 eV 2 scale lacked for many years experimental confirmation or refutation. The primary goal of the MiniBooNE experiment [2] is to address this anomaly in an unambiguous and independent way. The MiniBooNE flux is obtained via a high-intensity, conventional neutrino beam. Secondary hadrons, mostly pions and kaons, are produced via the interactions of 8 GeV protons from the Fermilab Booster accelerator with a thick beryllium target, and are focused by a horn. The switchable horn polarity allows for both neutrino and antineutrino running modes. The neutrino beam is produced via the decay of secondary mesons and muons in a 50 m long decay region. Overall, about 9.5 x 10 20 protons on target have been accumulated over the five years of beamline operation, 5.6 x 10 20 of which are used in this oscillation analysis, based on the neutrino running mode sample only. The MiniBooNE detector is located 540 m away from the beryllium target. The detector is a 12 m in diameter sphere filled with 800 t of undoped mineral oil, whose inner region is instrumented with 1280 photomultiplier tubes (PMTs). Neutrino interactions produce prompt, ring-distributed Cherenkov light, and delayed, isotropic scintillation light. Light transmission is affected by fluorescence, scattering, absorption and reflections. The outer detector region is used to reject cosmic ray activity or uncontained neutrino interactions. About 7.7 x 10 5 neutrino interactions have been collected at MiniBooNE. The goal of the first MiniBooNE electron appearance analysis is two-fold: perform a model-independent search for a ν e excess (or deficit), and interpret the data within a two neutrino, appearance-only ν μ → ν e oscillation context, to test this

  2. Rapid internalization of the oncogenic K+ channel K(V10.1.

    Directory of Open Access Journals (Sweden)

    Tobias Kohl

    Full Text Available K(V10.1 is a mammalian brain voltage-gated potassium channel whose ectopic expression outside of the brain has been proven relevant for tumor biology. Promotion of cancer cell proliferation by K(V10.1 depends largely on ion flow, but some oncogenic properties remain in the absence of ion permeation. Additionally, K(V10.1 surface populations are small compared to large intracellular pools. Control of protein turnover within cells is key to both cellular plasticity and homeostasis, and therefore we set out to analyze how endocytic trafficking participates in controlling K(V10.1 intracellular distribution and life cycle. To follow plasma membrane K(V10.1 selectively, we generated a modified channel of displaying an extracellular affinity tag for surface labeling by α-bungarotoxin. This modification only minimally affected K(V10.1 electrophysiological properties. Using a combination of microscopy and biochemistry techniques, we show that K(V10.1 is constitutively internalized involving at least two distinct pathways of endocytosis and mainly sorted to lysosomes. This occurs at a relatively fast rate. Simultaneously, recycling seems to contribute to maintain basal K(V10.1 surface levels. Brief K(V10.1 surface half-life and rapid lysosomal targeting is a relevant factor to be taken into account for potential drug delivery and targeting strategies directed against K(V10.1 on tumor cells.

  3. TU Berlin Rover Family for Terrestrial Testing of Complex Planetary Mission Scenarios

    Science.gov (United States)

    Kryza, L.; Brieß, K.

    2018-04-01

    The TU Berlin has developed a family of planetary rovers for educational use and research activities. The paper will introduce these cost-effective systems, which can be used for analogue mission demonstration on Earth.

  4. Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Stephanie O. Palmer

    2013-01-01

    Full Text Available We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interaction of EF-Tu with GDP in the absence of EF-Ts were observed to be = 33 μM, = 0.003 s−1, and the specificity constant was  s−1 μM−1. In the presence of EF-Ts, these values were shifted to = 2 μM, = 0.005 s−1, and the specificity constant was  s−1 μM−1. The equilibrium dissociation constants governing the binding of EF-Tu to GDP ( were 30–75 nM and to GTP ( were 125–200 nM. EF-Ts stimulated the exchange of GDP by EF-Tu 10-fold. P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U-dependent binding of Phe-tRNAPhe at the A-site of P. aeruginosa ribosomes. P. aeruginosa EF-Tu was active in poly(U-programmed polyphenylalanine protein synthesis system composed of all P. aeruginosa components.

  5. A generalized Tu formula and Hamiltonian structures of fractional AKNS hierarchy

    International Nuclear Information System (INIS)

    Wu, Guo-cheng; Zhang, Sheng

    2011-01-01

    In this Letter, a generalized Tu formula is firstly presented to construct Hamiltonian structures of fractional soliton equations. The obtained results can be reduced to the classical Hamiltonian hierarchy of AKNS in ordinary calculus. -- Highlights: → A generalized Tu formula is first established based on the fractional variational theory for non-differentiable functions. → Hamiltonian structures of fractional AKNS hierarchy are obtained. → The classical AKNS hierarchy is just a special case of the fractional hierarchy.

  6. A generalized Tu formula and Hamiltonian structures of fractional AKNS hierarchy

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Guo-cheng, E-mail: wuguocheng2002@yahoo.com.cn [Key Laboratory of Numerical Simulation of Sichuan Province, Neijiang, Sichuan 641112 (China); College of Mathematics and Information Science, Neijiang Normal University, Neijiang, Sichuan 641112 (China); Zhang, Sheng, E-mail: zhshaeng@yahoo.com.cn [School of Mathematical Sciences, Dalian University of Technology, Dalian 116024 (China)

    2011-10-03

    In this Letter, a generalized Tu formula is firstly presented to construct Hamiltonian structures of fractional soliton equations. The obtained results can be reduced to the classical Hamiltonian hierarchy of AKNS in ordinary calculus. -- Highlights: → A generalized Tu formula is first established based on the fractional variational theory for non-differentiable functions. → Hamiltonian structures of fractional AKNS hierarchy are obtained. → The classical AKNS hierarchy is just a special case of the fractional hierarchy.

  7. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-11, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    Dette notat dokumenterer de leverede data i datasættet TU 2006-11 version 2, som omfatter data fra perioden maj 2006 til 30. April 2012. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger såvidt muligt datasættet og opdateres ikke herefter....

  8. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-12, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    Dette notat dokumenterer de leverede data i datasættet TU 2006-12 version 2, som omfatter data fra perioden maj 2006 til 30. april 2012. For den mest opdaterede dokumentation af nyeste TU-data henvises til vores hjemmeside. Nærværende notat følger såvidt muligt datasættet og opdateres ikke herefter....

  9. The intensive DT neutron generator of TU Dresden

    Science.gov (United States)

    Klix, Axel; DÖring, Toralf; Domula, Alexander; Zuber, Kai

    2018-01-01

    TU Dresden operates an accelerator-based intensive DT neutron generator. Experimental activities comprise investigation into material activation and decay, neutron and photon transport in matter and R&D work on radiation detectors for harsh environments. The intense DT neutron generator is capable to produce a maximum of 1012 n/s. The neutron source is a solid-type water-cooled tritium target based on a titanium matrix on a copper carrier. The neutron yield at a typical deuteron beam current of 1 mA is of the order of 1011 n/s in 4Π. A pneumatic sample transport system is available for short-time irradiations and connected to wo high-purity germanium detector spectrometers for the measurement of induced activities. The overall design of the experimental hall with the neutron generator allows a flexible setup of experiments including the possibility of investigating larger structures and cooled samples or samples at high temperatures.

  10. The transient outward current in mice lacking the potassium channel gene Kv1.4

    Science.gov (United States)

    London, Barry; Wang, Dao W; Hill, Joseph A; Bennett, Paul B

    1998-01-01

    The transient outward current (Ito) plays a prominent role in the repolarization phase of the cardiac action potential. Several K+ channel genes, including Kv1.4, are expressed in the heart, produce rapidly inactivating currents when heterologously expressed, and may be the molecular basis of Ito.We engineered mice homozygous for a targeted disruption of the K+ channel gene Kv1.4 and compared Ito in wild-type (Kv1.4+/+), heterozygous (Kv1.4+/-) and homozygous ‘knockout’ (Kv1.4−/−) mice. Kv1.4 RNA was truncated in Kv1.4−/− mice and protein expression was absent.Adult myocytes isolated from Kv1.4+/+, Kv1.4+/− and Kv1.4−/− mice had large rapidly inactivating outward currents. The peak current densities at 60 mV (normalized by cellular capacitance, in pA pF−1; means ± s.e.m.) were 53.8 ± 5.3, 45.3 ± 2.2 and 44.4 ± 2.8 in cells from Kv1.4+/+, Kv1.4+/− and Kv1.4−/− mice, respectively (P mice.The voltage dependence and time course of inactivation were not changed by targeted disruption of Kv1.4. The mean best-fitting V½ (membrane potential at 50 % inactivation) values for myocytes from Kv1.4 +/+, Kv1.4+/− and Kv1.4−/− mice were -53.5 ± 3.7, -51.1 ± 2.6 and -54.2 ± 2.4 mV, respectively. The slope factors (k) were -10.1 ± 1.4, -8.8 ± 1.4 and -9.5 ± 1.2 mV, respectively. The fast time constants for development of inactivation at -30 mV were 27.8 ± 2.2, 26.2 ± 5.1 and 19.6 ± 2.1 ms in Kv1.4+/+, Kv1.4+/− and Kv1.4−/− myocytes, respectively. At +30 mV, they were 35.5 ± 2.6, 30.0 ± 2.1 and 28.7 ± 1.6 ms, respectively. The time constants for the rapid phase of recovery from inactivation at -80 mV were 32.5 ± 8.2, 23.3 ± 1.8 and 39.0 ± 3.7 ms, respectively.Nearly the entire inactivating component as well as more than 60 % of the steady-state outward current was eliminated by 1 mm 4-aminopyridine in Kv1.4+/+, Kv1.4+/− and Kv1.4−/− myocytes.Western blot analysis of heart membrane extracts showed no significant

  11. 735 kV circuit breakers for ehv

    Energy Technology Data Exchange (ETDEWEB)

    1966-01-01

    French manufacturers have been studying the design of high and extra high voltage circuit breakers for several years. The two techniques they used were the low volume oil and the air pressure technique. These have permitted the development of a type gear capable of solving problems all over the world as they arose following the development of electrical energy transmission at extra high voltage as well as high short circuit power. Today, the air pressure solution is used in new constructions such as the 735 kV transmission network in Canada.

  12. Design and testing of reactors for 735 kV

    Energy Technology Data Exchange (ETDEWEB)

    Erb, W; Kraaij, D J

    1965-11-01

    The design and testing of five large, single phase shunt reactors rated either 110 or 55 MVAR, supplied for the 735 kV system of the Quebec Hydro Electric Commission which came into operation in the autumn of 1965 are described. As these reactors are permanently connected to the transmission lines, their losses must be considered as being continuously present and must be determined exactly. In addition to the use of a new bridge method, the losses were also measured calorimetrically for the purpose of comparison, the agreement between the two tests being remarkably good. The impulse tests with full wave and chopped wave are subsequently described.

  13. Chemical Composition of RR Lyn – an Eclipsing Binary System with Am and λ Boo Type Components

    Directory of Open Access Journals (Sweden)

    Yeuncheol Jeong

    2017-06-01

    Full Text Available High-resolution spectroscopic observations of the eclipsing binary system RR Lyn were made using the 1.8 m telescope at the Bohuynsan Optical Astronomical Observatory in Korea. The spectral resolving power was R = 82,000, with a signal to noise ratio of S/N > 150. We found the effective temperatures and surface gravities of the primary and secondary components to be equal to Teff = 7,920 & 7,210 K and log(g = 3.80 & 4.16, respectively. The abundances of 34 and 17 different chemical elements were found in the atmospheric components. Correlations between the derived abundances with condensation temperatures and the second ionization potentials of these elements are discussed. The primary component is a typical metallic line star with the abundances of light and iron group elements close to solar values, while elements with atomic numbers Z > 30 are overabundant by 0.5–1.5 dex with respect to solar values. The secondary component is a λ Boo type star. In this type of stars, CNO abundances are close to solar values, while the abundance pattern shows a negative correlation with condensation temperatures.

  14. TaBoo SeArch Algorithm with a Modified Inverse Histogram for Reproducing Biologically Relevant Rare Events of Proteins.

    Science.gov (United States)

    Harada, Ryuhei; Takano, Yu; Shigeta, Yasuteru

    2016-05-10

    The TaBoo SeArch (TBSA) algorithm [ Harada et al. J. Comput. Chem. 2015 , 36 , 763 - 772 and Harada et al. Chem. Phys. Lett. 2015 , 630 , 68 - 75 ] was recently proposed as an enhanced conformational sampling method for reproducing biologically relevant rare events of a given protein. In TBSA, an inverse histogram of the original distribution, mapped onto a set of reaction coordinates, is constructed from trajectories obtained by multiple short-time molecular dynamics (MD) simulations. Rarely occurring states of a given protein are statistically selected as new initial states based on the inverse histogram, and resampling is performed by restarting the MD simulations from the new initial states to promote the conformational transition. In this process, the definition of the inverse histogram, which characterizes the rarely occurring states, is crucial for the efficiency of TBSA. In this study, we propose a simple modification of the inverse histogram to further accelerate the convergence of TBSA. As demonstrations of the modified TBSA, we applied it to (a) hydrogen bonding rearrangements of Met-enkephalin, (b) large-amplitude domain motions of Glutamine-Binding Protein, and (c) folding processes of the B domain of Staphylococcus aureus Protein A. All demonstrations numerically proved that the modified TBSA reproduced these biologically relevant rare events with nanosecond-order simulation times, although a set of microsecond-order, canonical MD simulations failed to reproduce the rare events, indicating the high efficiency of the modified TBSA.

  15. A Measurement of the Neutrino Neutral Current π0 Cross Section at MiniBooNE

    Energy Technology Data Exchange (ETDEWEB)

    Raaf, Jennifer Lynne [Univ. of Cincinnati, OH (United States)

    2005-01-01

    The MiniBooNE neutrino beam and detector at Fermilab are used to study the production of neutral current π0 events. The cross sections for neutrino interactions with mineral oil (CH2) are reported for resonantly produced and coherently produced single π0 events. We measure a resonant single π0 cross section of σ(vμ N π0) = (0.0129 ± 0.0011(stat.) ± 0.0043(syst.)) x 10-36 cm2/CH2 at a mean neutrino energy of 1.26 GeV. We measure a coherent single π0 cross section of σ(vμ A → vμ A π0) = (0.00077 ± 0.00016 (stat.) ± 0.00036 (syst.)) x 10-36 cm2/CH2 at mean neutrino energy 1.12 GeV.

  16. A neutron scattering study of the ternary complex EF-Tu.GTP-valyl-tRNAVal1A

    DEFF Research Database (Denmark)

    Österberg, R.; Elias, P.; Kjems, Jørgen

    1986-01-01

    The complex formation between elongation factor Tu (EF-Tu), GTP, and valyl-tRNAVal1A has been investigated in a hepes buffer of "pH" 7.4 and 0.2 M ionic strength using the small-angle neutron scattering method at concentrations of D2O where EF-Tu (42% D2O) and tRNA (71% D2O) are successively...

  17. Time-, Sex-, and Dose-Dependent Alterations of the Gut Microbiota by Consumption of Dietary Daikenchuto (TU-100

    Directory of Open Access Journals (Sweden)

    Jun Miyoshi

    2018-01-01

    Full Text Available Medications or dietary components can affect both the host and the host’s gut microbiota. Changes in the microbiota may influence medication efficacy and interactions. Daikenchuto (TU-100, a herbal medication, comprised of ginger, ginseng, and Japanese pepper, is widely used in Japanese traditional Kampo medicine for intestinal motility and postoperative paralytic ileus. We previously showed in mice that consumption of TU-100 for 4 weeks changed the gut microbiota and increased bioavailability of bacterial ginsenoside metabolites. Since TU-100 is prescribed in humans for months to years, we examined the time- and sex-dependent effects of TU-100 on mouse gut microbiota. Oral administration of 1.5% TU-100 for 24 weeks caused more pronounced changes in gut microbiota in female than in male mice. Changes in both sexes largely reverted to baseline upon TU-100 withdrawal. Effects were time and dose dependent. The microbial profiles reverted to baseline within 4 weeks after withdrawal of 0.75% TU-100 but were sustained after withdrawal of 3% TU-100. In summary, dietary TU-100 changed mouse microbiota in a time-, sex-, and dose-dependent manner. These findings may be taken into consideration when determining optimizing dose for conditions of human health and disease with the consideration of differences in composition and response of the human intestinal microbiota.

  18. The C-terminal HRET sequence of Kv1.3 regulates gating rather than targeting of Kv1.3 to the plasma membrane.

    Science.gov (United States)

    Voros, Orsolya; Szilagyi, Orsolya; Balajthy, András; Somodi, Sándor; Panyi, Gyorgy; Hajdu, Péter

    2018-04-12

    Kv1.3 channels are expressed in several cell types including immune cells, such as T lymphocytes. The targeting of Kv1.3 to the plasma membrane is essential for T cell clonal expansion and assumed to be guided by the C-terminus of the channel. Using two point mutants of Kv1.3 with remarkably different features compared to the wild-type Kv1.3 (A413V and H399K having fast inactivation kinetics and tetraethylammonium-insensitivity, respectively) we showed that both Kv1.3 channel variants target to the membrane when the C-terminus was truncated right after the conserved HRET sequence and produce currents identical to those with a full-length C-terminus. The truncation before the HRET sequence (NOHRET channels) resulted in reduced membrane-targeting but non-functional phenotypes. NOHRET channels did not display gating currents, and coexpression with wild-type Kv1.3 did not rescue the NOHRET-A413V phenotype, no heteromeric current was observed. Interestingly, mutants of wild-type Kv1.3 lacking HRET(E) (deletion) or substituted with five alanines for the HRET(E) motif expressed current indistinguishable from the wild-type. These results demonstrate that the C-terminal region of Kv1.3 immediately proximal to the S6 helix is required for the activation gating and conduction, whereas the presence of the distal region of the C-terminus is not exclusively required for trafficking of Kv1.3 to the plasma membrane.

  19. Wild Horse 69-kV transmission line environmental assessment

    International Nuclear Information System (INIS)

    1996-12-01

    Hill County Electric Cooperative Inc. (Hill County) proposes to construct and operate a 69-kV transmission line from its North Gildford Substation in Montana north to the Canadian border. A vicinity project area map is enclosed as a figure. TransCanada Power Corporation (TCP), a Canadian power-marketing company, will own and construct the connecting 69-kV line from the international border to Express Pipeline's pump station at Wild Horse, Alberta. This Environmental Assessment is prepared for the Department of Energy (DOE) as lead federal agency to comply with the requirements of the National Environmental Policy Act (NEPA), as part of DOE's review and approval process of the applications filed by Hill County for a DOE Presidential Permit and License to Export Electricity to a foreign country. The purpose of the proposed line is to supply electric energy to a crude oil pump station in Canada, owned by Express Pipeline Ltd. (Express). The pipeline would transport Canadian-produced oil from Hardisty, Alberta, Canada, to Caster, Wyoming. The Express Pipeline is scheduled to be constructed in 1996--97 and will supply crude oil to refineries in Wyoming and the midwest

  20. Corona performance of a compact 230-kV line

    International Nuclear Information System (INIS)

    Chartier, V.L.; Blair, D.E.

    1994-01-01

    Permitting requirements and the acquisition of new rights-of-way for transmission facilities has in recent years become increasingly difficult for most utilities, including Puget Sound Power and Light Company. In order to maintain a high degree of reliability of service while being responsive to public concerns regarding the siting of high voltage (HV) transmission facilities, Puget Power has found it necessary to more heavily rely upon the use of compact lines in franchise corridors. Compaction does, however, precipitant increased levels of audible noise (AN) and radio and TV interference (RI and TVI) due to corona on the conductors and insulator assemblies. Puget Power relies upon the Bonneville Power Administration (BPA) Corona and Field Effects computer program to calculate AN and RI for new lines. Since there was some question of the program's ability to accurately represent quiet 230-kV compact designs, a joint project was undertaken with BPA to verify the program's algorithms. Long-term measurements made on an operating Puget Power 230-kV compact line confirmed the accuracy of BPA's AN model; however, the RI measurements were much lower than predicted by the BPA computer and other programs. This paper also describes how the BPA computer program can be used to calculate the voltage needed to expose insulator assemblies to the correct electric field in single test setups in HV laboratories

  1. A continuous acceleration tube of ions under 200 KV

    International Nuclear Information System (INIS)

    Mongodin, G.

    1954-01-01

    The realization of an Van de Graaff accelerator required, for the preliminary studies, the construction of a small proton accelerator, functioning at 200 kV in order to resolve some parasitic effects inherent to the accelerators tubes. The aim of this report is to describe the different organs of the accelerator tube, to explain the operating system and to encode their characteristics. The report first presents the ion source and the beam buncher permitting to inject in the accelerator tube particles of about 9 kV and very batched in a thin beam of circular section. Then the study explain the tube characteristics considered like optic system. A method to obtain precise calculation of particle trajectories is exposed. Aberrations of the system were discussed and balance of the currents on all electrodes inside the tube for different regimes of working were provided. The influence of the residual pressure in the tube were explained. The report finally ends on a part of the fundamental problem of the straining occurring inside the tubes accelerators under high tension. (M.B.) [fr

  2. Wild Horse 69-kV transmission line environmental assessment

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    Hill County Electric Cooperative Inc. (Hill County) proposes to construct and operate a 69-kV transmission line from its North Gildford Substation in Montana north to the Canadian border. A vicinity project area map is enclosed as a figure. TransCanada Power Corporation (TCP), a Canadian power-marketing company, will own and construct the connecting 69-kV line from the international border to Express Pipeline`s pump station at Wild Horse, Alberta. This Environmental Assessment is prepared for the Department of Energy (DOE) as lead federal agency to comply with the requirements of the National Environmental Policy Act (NEPA), as part of DOE`s review and approval process of the applications filed by Hill County for a DOE Presidential Permit and License to Export Electricity to a foreign country. The purpose of the proposed line is to supply electric energy to a crude oil pump station in Canada, owned by Express Pipeline Ltd. (Express). The pipeline would transport Canadian-produced oil from Hardisty, Alberta, Canada, to Caster, Wyoming. The Express Pipeline is scheduled to be constructed in 1996--97 and will supply crude oil to refineries in Wyoming and the midwest.

  3. Expression and function of K(V)2-containing channels in human urinary bladder smooth muscle.

    Science.gov (United States)

    Hristov, Kiril L; Chen, Muyan; Afeli, Serge A Y; Cheng, Qiuping; Rovner, Eric S; Petkov, Georgi V

    2012-06-01

    The functional role of the voltage-gated K(+) (K(V)) channels in human detrusor smooth muscle (DSM) is largely unexplored. Here, we provide molecular, electrophysiological, and functional evidence for the expression of K(V)2.1, K(V)2.2, and the electrically silent K(V)9.3 subunits in human DSM. Stromatoxin-1 (ScTx1), a selective inhibitor of K(V)2.1, K(V)2.2, and K(V)4.2 homotetrameric channels and of K(V)2.1/9.3 heterotetrameric channels, was used to examine the role of these channels in human DSM function. Human DSM tissues were obtained during open bladder surgeries from patients without a history of overactive bladder. Freshly isolated human DSM cells were studied using RT-PCR, immunocytochemistry, live-cell Ca(2+) imaging, and the perforated whole cell patch-clamp technique. Isometric DSM tension recordings of human DSM isolated strips were conducted using tissue baths. RT-PCR experiments showed mRNA expression of K(V)2.1, K(V)2.2, and K(V)9.3 (but not K(V)4.2) channel subunits in human isolated DSM cells. K(V)2.1 and K(V)2.2 protein expression was confirmed by Western blot analysis and immunocytochemistry. Perforated whole cell patch-clamp experiments revealed that ScTx1 (100 nM) inhibited the amplitude of the voltage step-induced K(V) current in freshly isolated human DSM cells. ScTx1 (100 nM) significantly increased the intracellular Ca(2+) level in DSM cells. In human DSM isolated strips, ScTx1 (100 nM) increased the spontaneous phasic contraction amplitude and muscle force, and enhanced the amplitude of the electrical field stimulation-induced contractions within the range of 3.5-30 Hz stimulation frequencies. These findings reveal that ScTx1-sensitive K(V)2-containing channels are key regulators of human DSM excitability and contractility and may represent new targets for pharmacological or genetic intervention for bladder dysfunction.

  4. Contribution of kv7.4/kv7.5 heteromers to intrinsic and calcitonin gene-related Peptide-induced cerebral reactivity

    DEFF Research Database (Denmark)

    Chadha, Preet S; Jepps, Thomas A; Carr, Georgina

    2014-01-01

    Middle cerebral artery (MCA) diameter is regulated by inherent myogenic activity and the effect of potent vasodilators such as calcitonin gene-related peptide (CGRP). Previous studies showed that MCAs express KCNQ1, 4, and 5 potassium channel genes, and the expression products (Kv7 channels) part......) participate in the myogenic control of MCA diameter. The present study investigated the contribution of Kv7.4 and Kv7.5 isoforms to myogenic and CGRP regulation of MCA diameter and determined whether they were affected in hypertensive animals....

  5. High power thyristors with 5 kV blocking voltage. Volume 1: Development of high-voltage-thyristors (4.5 kV) with good dynamic properties

    Science.gov (United States)

    Lock, K.; Patalong, H.; Platzoeder, K.

    1979-01-01

    Using neutron irradiated silicon with considerably lower spread in resistivity as compared to conventionally doped silicon it was possible to produce power thyristors with breakdown voltages between 3.5 kV and 5.5 kV. The thyristor pellets have a diameter of 50 mm. Maximum average on-state currents of 600 to 800 A can be reached with these elements. The dynamic properties of the thryistors could be improved to allow standard applications up to maximum repetitive voltages of 4.5 kV.

  6. The pan-Kv7 (KCNQ) Channel Opener Retigabine Inhibits Striatal Excitability by Direct Action on Striatal Neurons In Vivo

    DEFF Research Database (Denmark)

    Hansen, Henrik H; Weikop, Pia; Mikkelsen, Maria D

    2017-01-01

    Central Kv7 (KCNQ) channels are voltage-dependent potassium channels composed of different combinations of four Kv7 subunits, being differently expressed in the brain. Notably, striatal dopaminergic neurotransmission is strongly suppressed by systemic administration of the pan-Kv7 channel opener ...... by acute systemic haloperidol administration in the rat. The relative mRNA levels of Kv7 subunits in the rat striatum were found to be Kv7.2 = Kv7.3 = Kv7.5 > >Kv7.4. These data suggest that intrastriatal Kv7 channels play a direct role in regulating striatal excitability in vivo....

  7. 400 kV injector compact ECR ion source

    International Nuclear Information System (INIS)

    Constantin, F.; Catana, D.; Macovei, M.; Ivanov, E.

    1997-01-01

    Obtaining multiple ionised ions is a fundamental problem for some applications and research. Multiple ionised ions can be produced from electronic bombardment, when n·τ≥5·10 9 cm -3 · s, where n is the density of electrons (in cm -3 ) and τ is the time of interaction between electrons and ions . The relative speed of electrons and ions determines the equilibrium between the stripping process of the atom's electrons and their capture. An ion source with high ionisation efficiency and large output current is the ECR source (Electron Cyclotron Resonance). Using an ECR source with permanent magnets as ion source for the injector will lead to following advantages: - the possibility to obtain multiple ionised particles; - an increase of ion beam intensities; - the expanding of accelerator activities; - a longer working time, due to magnetron lifetime. The ECR ion source is robust, compact and capable of high intensities of extracted ion current. The large functional domain for the residual gas pressure allows the production of multiple charged ions. The source can be easily integrated in the TRILAC's injection structure. We realised a compact microwave ion source which has an axial magnetic field generated by a permanent magnet of Co-Sm. 1200 G magnetic field is greater than the 875 G magnetic field corresponding to the electron-cyclotron frequency of 2.45 GHz. The microwave generator is a magnetron (2.45 GHz and 200 W in continuos wave). The microwave is fed through a coaxial connector on the top of flange. The test was made on He gas at a pressure between 8· 10 -4 and 5·10 -2 torr. The ion beam current was measured vs. extracted potential from 3 kV to 10 kV and has a dependence according to U 3/2 law. A maximal ion current of 300 μA at 10 kV extraction potential was measured. Dimension of ECR ion source, including Einzel lens are φ=12 cm and h=16 cm. (authors)

  8. A model for the Global Quantum Efficiency for a TPB-based wavelength-shifting system used with photomultiplier tubes in liquid argon in MicroBooNE

    Science.gov (United States)

    Pate, S. F.; Wester, T.; Bugel, L.; Conrad, J.; Henderson, E.; Jones, B. J. P.; McLean, A. I. L.; Moon, J. S.; Toups, M.; Wongjirad, T.

    2018-02-01

    We present a model for the Global Quantum Efficiency (GQE) of the MicroBooNE optical units. An optical unit consists of a flat, circular acrylic plate, coated with tetraphenyl butadiene (TPB), positioned near the photocathode of a 20.2-cm diameter photomultiplier tube. The plate converts the ultra-violet scintillation photons from liquid argon into visible-spectrum photons to which the cryogenic phototubes are sensitive. The GQE is the convolution of the efficiency of the plates that convert the 128 nm scintillation light from liquid argon to visible light, the efficiency of the shifted light to reach the photocathode, and the efficiency of the cryogenic photomultiplier tube. We develop a GEANT4-based model of the optical unit, based on first principles, and obtain the range of probable values for the expected number of detected photoelectrons (NPE) given the known systematic errors on the simulation parameters. We compare results from four measurements of the NPE determined using alpha-particle sources placed at two distances from a TPB-coated plate in a liquid argon cryostat test stand. We also directly measured the radial dependence of the quantum efficiency, and find that this has the same shape as predicted by our model. Our model results in a GQE of 0.0055±0.0009 for the MicroBooNE optical units. While the information shown here is MicroBooNE specific, the approach to the model and the collection of simulation parameters will be widely applicable to many liquid-argon-based light collection systems.

  9. Discharge Against Medical Advice in the Pediatric Wards in Boo-ali Sina Hospital, Sari, Iran 2010.

    Science.gov (United States)

    Mohseni Saravi, Benyamin; Reza Zadeh, Esmaeil; Siamian, Hasan; Yahghoobian, Mahboobeh

    2013-12-01

    Since children neither comprehended nor contribute to the decision, discharge against medical advice is a challenge of health care systems in the world. Therefore, the current study was designed to determine the rate and causes of discharge against medical advice. This descriptive cross-sectional study was done by reviewing the medical records by census method. Data was analyzed using SPSS software and x(2) statistics was used to determine the relationship between variables. The value of P<0.05 was considered significant. Rate of discharged against medical advice was 108 (2.2%). Mean of age and length of stay were 2.8±4 (SD).3 years old and 3.7±5.4 (SD) days, respectively. Totally, 95 patients (88.7%) had health insurance and 65 (60.2%) patients lived in urban areas. History of psychiatric disease and addiction in 22 (20.6%) of the parents were negative. In addition, 100 (92.3%) patients admitted for medical treatment and the others for surgery. The relationship of the signatory with patients (72.3%) was father. Of 108 patients discharged against medical advice, 20 (12%) were readmitted. The relationship between the day of discharge and discharge against medical advice was significant (ρ =0/03). Rate of discharge against medical advice in Boo-ali hospital is the same as the other studies in the same range. The form which is used for this purpose did not have suitable data elements about description of consequence of such discharge, and it has not shown the real causes of discharge against medical advice.

  10. Asymptomatic bacteriuria in pregnant women attending Boo-Ali Hospital Tehran Iran: Urine analysis vs. urine culture.

    Science.gov (United States)

    Etminan-Bakhsh, Mina; Tadi, Sima; Darabi, Roksana

    2017-11-01

    Asymptomatic bacteriuria is one of the common problems in pregnancy. Asymptomatic bacteriuria is associated with pyelonephritis, preterm labor and low birth weight infants. The physiological and anatomical changes in pregnancy facilitate urinary tract infection (UTI) during pregnancy. Several tests are available for diagnosis of asymptomatic bacteriuria. The urine culture is a gold standard diagnostic test for asymptomatic bacteriuria but it is expensive and time-consuming. Screening methods may be useful in detecting high-risk pregnant women for asymptomatic bacteriuria. The aim of the present study was to compare urine analysis as a rapid screening test to urine culture in diagnosis of asymptomatic bacteriuria. A total of 123 pregnant women attending the obstetrics clinic of Boo-Ali hospital in Tehran, Iran from March 2013 to September 2014 were included in the present diagnostic cross-sectional study. One hundred twenty three mid-stream urine samples were inoculated into cultures and were processed by dipstick (nitrite test and leucocyte esterase test) and microscopic pus cell count. The sensitivity, specificity, positive predictive value and negative predictive value of nitrite test, leucocyte esterase test and microscopic pus cell count were compared with urine culture in diagnosis of asymptomatic bacteriuria by using SPSS version 19. Of 123 urine samples, significant asymptomatic bacteriuria (≥10 4 cfu/Ml) was detected in 8 (6.5%) subjects. The sensitivity and specificity of nitrite test were 37% and 100% respectively. The sensitivity of pus cell count alone and leucocyte esterase test alone were 100% but the specificity of them were 64% and 65% respectively. We found high negative predictive value by Pus cell count and the leucocyte esterase test (100%) and low positive predictive value by them (16% and 17% respectively). Urine culture is the most useful test for diagnosis of asymptomatic bacteriuria. None of our screening tests had a sensitivity and

  11. The short-circuit test results of 6.9 kV/2.3 kV 400 kVA-class YBCO model transformer

    International Nuclear Information System (INIS)

    Tomioka, A.; Otonari, T.; Ogata, T.; Iwakuma, M.; Okamoto, H.; Hayashi, H.; Iijima, Y.; Saito, T.; Gosho, Y.; Tanabe, K.; Izumi, T.; Shiohara, Y.

    2011-01-01

    The 6.9 kV/2.3 kV 400 kVA-class single-phase YBCO model transformer with the YBCO tape with copper tape was manufactured for short-circuit current test. Short-circuit test was performed and the short-circuit current of primary winding was 346 A which was about six times larger than the rated current. The I-V characteristics of the winding did not change before and after the test. The transformer withstood short-circuit current. We are planning to turn the result into a consideration of a 66 kV/6.9 kV-20 MVA-class three-phase superconducting transformer. We are developing an elemental technology for 66 kV/6.9 kV 20 MVA-class power transformer with YBCO conductors. The protection of short-circuit technology is one of the elemental technologies for HTS transformer. Since short-circuit current is much higher than critical current of YBCO tape, there is a possibility that superconducting characteristics may be damaged during short-circuit period. We made a conductor to compose the YBCO tape with copper tape. We manufactured 6.9 kV/2.3 kV 400 kVA-class YBCO model transformer using this conductor and performed short-circuit current test. The short-circuit current of primary winding was 346 A which was about six times larger than the rated current. The I-V characteristics of the winding did not change before and after the test. We may consider this conductor withstands short-circuit current.

  12. 100 kV reliable accurately-synchronized spark gap

    International Nuclear Information System (INIS)

    Bosamykin, V.S.; Gerasimov, A.I.; Zenkov, D.I.

    1987-01-01

    100 kV three-electrode spark gap filled with 40% SF 6 +60% N 2 mixture under the pressure of ∼ 1 MPa, which has spread Δt ≤ ± 5 ns of operating time delay in the range of 10 4 triggerings and commutation energy of 2.5 kJ, providing electric strength is 100%, is described; at 10 kJ Δt is less than ± 10 ns for 10 3 triggerings. Parallel connection of 16 groups, each consisting of 5 spark gaps with series connection, electric strength being 100%, in the pulse charging unit of Arkadiev-Marx generator being in operation for several years manifested their high efficiency; mutual group spread is ≤ ± 15 ns

  13. Do kV really make everything look grey? A comparative study of 600 kV computed tomography systems; Macht kV wirklich grau? Eine Vergleichsstudie zu 600 kV Computertomografiesystemen

    Energy Technology Data Exchange (ETDEWEB)

    Krumm, Michael; Sauerwein, Christoph; Haemmerle, Volker; Knupe, Gunnar [RayScan Technologies GmbH, Meersburg (Germany)

    2013-07-01

    In the automotive industry there is a manufacture of cast aluminium parts of considerable thickness. Growing importance is being attached to methods of nondestructive testing and full metrological characterisation of these parts. A major contribution to this end has been made by the latest generation of CT systems, whose 600 kV X-ray tubes and line detectors make for excellent image quality. The purpose of the present study was to make an objective assessment of the performance of these CT systems by examining the advantages and drawbacks of each. This included comparisons of various parameters such as tube voltage and exposure time as well as different tube and detector technologies and CT measurement methods.

  14. MFTF 230 kV pulsed power substation

    International Nuclear Information System (INIS)

    Wilson, J.H.

    1979-01-01

    The Mirror Fusion Test Facility (MFTF) currently under construction at the Lawrence Livermore Laboratory includes a Sustaining Neutral Beam Power Supply System (SNBPSS) consisting of 24 power-supply sets. The System will operate in long pulses (initially .5 seconds and eventually 30 seconds) at high power (200 MW), which will necessitate a large source of ac power. To meet this requirement, a new 230-kV substation is also being built at LLL. The constraints of cost, equipment protection, short operating lifetime (10 years), and reliability dictated a unique substation design. Its unusual features include provisions for fast fault detection and tripping, a capability for limiting ground fault current, low impedance, and economical design

  15. Faraday Rotator 5 kV Capacitor Bank

    International Nuclear Information System (INIS)

    Fetterman, C.C.

    1975-01-01

    A Faraday rotator 5 kV capacitor bank is a pulsed output power supply used to energize Faraday rotators for optical isolation in the ''LLL kJ Glass Laser System.'' Each supply contains either one, two or three parallel 240 μF storage capacitors depending on the size of the isolator used. Generally, the ''A*''(216 μH) isolator is energized with one capacitor, the ''A''(116 μH) isolator uses two capacitors and the ''B''(87 μH) isolator requires three capacitors. All models of isolators have been tested with four capacitors under maximum voltage and 25 feet of RG-217 cable with no hazardous effects. Except for the number of capacitors in each unit, the supplies are otherwise physically identical

  16. Optimization of 200 kV electrostatic accelerator

    Directory of Open Access Journals (Sweden)

    M Nazmabadi

    2015-09-01

    Full Text Available Optimizations on 200 kV electrostatic accelerator have been done in order to increasing ion current on target, improving vacuum condition and reduction in x-rays emission, increasing stability of high voltage power supply and reaching much greater achievable voltage value. The accelerator tube has most important effect on beam tracing in the electrostatic accelerators. So precautions most be considered in designing and constructing of this part. In order to finding permissible tolerances in construction and assembling of 200 kV electrostatic accelerator column, first the effects of angle deviation of a part from accelerator axis on beam track in the accelerator tube was simulated with Simion 7.0 computer program. We found that in order to prevent beam lost, the tolerances of balancing and co-centering of each part should be smaller than 0.1 mm. Each part of accelerator tube constructed by tolerances lower than 0.05 mm. Ultrasonic cleaning method used in pre-assembling process of parts. Because of its excellences, in the new tube we used borosilicate glass instead of high density alumina as insulators between the metallic electrodes. After three days of working vacuum pumps the system reached to 8.0×10-7 and after months to 5.0×10-7 ultimate pressure values. Measurements showed that by these considerations the maximum of reachable ion current on target was 1.1 mA which increased 50% compared to old machine, while x-ray emission intensity was increased by 25%. Optimizations of high voltage power supply are now under studies and tests

  17. The voltage-gated potassium channel subunit, Kv1.3, is expressed in epithelia

    DEFF Research Database (Denmark)

    Grunnet, Morten; Rasmussen, Hanne B; Hay-Schmidt, Anders

    2003-01-01

    The Shaker-type voltage-gated potassium channel, Kv1.3, is believed to be restricted in distribution to lymphocytes and neurons. In lymphocytes, this channel has gained intense attention since it has been proven that inhibition of Kv1.3 channels compromise T lymphocyte activation. To investigate...

  18. PKC and AMPK regulation of Kv1.5 potassium channels

    DEFF Research Database (Denmark)

    Andersen, Martin Nybo; Skibsbye, Lasse; Tang, Chuyi

    2015-01-01

    The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells....

  19. harmonic load modeling: a case study of 33 kv abuja steel mill feeder

    African Journals Online (AJOL)

    HOD

    techniques are adopted. This paper studied the harmonic orders of the 33 kV Abuja Steel Feeder modeled as a ... (ETAP) software package was deployed to perform Discrete Fast Transform (DFT) while the input ... and documented in research and development articles ... network with 33kV Abuja Steel feeder as case study.

  20. Pharmacological dissection of K(v)7.1 channels in systemic and pulmonary arteries

    DEFF Research Database (Denmark)

    Chadha, Preet S; Zunke, Friederike; Davis, Alison J

    2012-01-01

    The aim of this study was to characterize the functional impact of KCNQ1-encoded voltage-dependent potassium channels (K(v)7.1) in the vasculature.......The aim of this study was to characterize the functional impact of KCNQ1-encoded voltage-dependent potassium channels (K(v)7.1) in the vasculature....

  1. Development of a 300-kV Marx generator and its application to drive ...

    Indian Academy of Sciences (India)

    Accelerator and Pulse Power Division, Bhabha Atomic Research Centre, ... anode gap of 7·5 mm, an REB having beam voltage 160 kV and duration 150 ns ... lowest stage has a triggered SG which is closed by a 6 kV pulse obtained with the ...

  2. A study on the insulation coordination of 765 kV system

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jeong Boo; Shim, Eung Bo [Korea Electric Power Corp. (KEPCO), Taejon (Korea, Republic of). Research Center; Lee, Yong Han; Youn, Jae Yeong; Hwang, Chi Woo; Jung, Dong Hak [Korea Electrotechnology Research Inst., Changwon (Korea, Republic of)

    1996-12-31

    Analysis of the power frequency temporary overvoltage. Analysis of switching surges - Fault imitation, closing and re closing, fault clearing. Analysis of lightning surges. Insulation design of 765 kV overhead transmission line. Insulation coordination of 765 kV gas insulated substation. Transient recovery voltage and high speed ground switch (author). 38 refs., 55 figs.

  3. Kv10.1 potassium channel: from the brain to the tumors.

    Science.gov (United States)

    Cázares-Ordoñez, V; Pardo, L A

    2017-10-01

    The KCNH1 gene encodes the Kv10.1 (Eag1) ion channel, a member of the EAG (ether-à-go-go) family of voltage-gated potassium channels. Recent studies have demonstrated that KCHN1 mutations are implicated in Temple-Baraitser and Zimmermann-Laband syndromes and other forms of developmental deficits that all present with mental retardation and epilepsy, suggesting that Kv10.1 might be important for cognitive development in humans. Although the Kv10.1 channel is mainly expressed in the mammalian brain, its ectopic expression occurs in 70% of human cancers. Cancer cells and tumors expressing Kv10.1 acquire selective advantages that favor cancer progression through molecular mechanisms that involve several cellular pathways, indicating that protein-protein interactions may be important for Kv10.1 influence in cell proliferation and tumorigenesis. Several studies on transcriptional and post-transcriptional regulation of Kv10.1 expression have shown interesting mechanistic insights about Kv10.1 role in oncogenesis, increasing the importance of identifying the cellular factors that regulate Kv10.1 expression in tumors.

  4. KCNE3 is an inhibitory subunit of the Kv4.3 potassium channel

    DEFF Research Database (Denmark)

    Lundby, Alicia; Olesen, Søren-Peter

    2006-01-01

    The mammalian Kv4.3 potassium channel is a fast activating and inactivating K+ channel widely distributed in mammalian tissues. Kv4.3 is the major component of various physiologically important currents ranging from A-type currents in the CNS to the transient outward potassium conductance in the ...

  5. From pan-reactive KV7 channel opener to subtype selective opener/inhibitor by addition of a methyl group.

    Directory of Open Access Journals (Sweden)

    Sigrid Marie Blom

    Full Text Available The voltage-gated potassium channels of the KV7 family (KV7.1-5 play important roles in controlling neuronal excitability and are therefore attractive targets for treatment of CNS disorders linked to hyperexcitability. One of the main challenges in developing KV7 channel active drugs has been to identify compounds capable of discriminating between the neuronally expressed subtypes (KV7.2-5, aiding the identification of the subunit composition of KV7 currents in various tissues, and possessing better therapeutic potential for particular indications. By taking advantage of the structure-activity relationship of acrylamide KV7 channel openers and the effects of these compounds on mutant KV7 channels, we have designed and synthesized a novel KV7 channel modulator with a unique profile. The compound, named SMB-1, is an inhibitor of KV7.2 and an activator of KV7.4. SMB-1 inhibits KV7.2 by reducing the current amplitude and increasing the time constant for the slow component of the activation kinetics. The activation of KV7.4 is seen as an increase in the current amplitude and a slowing of the deactivation kinetics. Experiments studying mutant channels with a compromised binding site for the KV7.2-5 opener retigabine indicate that SMB-1 binds within the same pocket as retigabine for both inhibition of KV7.2 and activation of KV7.4. SMB-1 may serve as a valuable tool for KV7 channel research and may be used as a template for further design of better subtype selective KV7 channel modulators. A compound with this profile could hold novel therapeutic potential such as the treatment of both positive and cognitive symptoms in schizophrenia.

  6. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability

    Science.gov (United States)

    Speca, David J.; Ogata, Genki; Mandikian, Danielle; Bishop, Hannah I.; Wiler, Steve W.; Eum, Kenneth; Wenzel, H. Jürgen; Doisy, Emily T.; Matt, Lucas; Campi, Katharine L.; Golub, Mari S.; Nerbonne, Jeanne M.; Hell, Johannes W.; Trainor, Brian C.; Sack, Jon T.; Schwartzkroin, Philip A.; Trimmer, James S.

    2014-01-01

    The Kv2.1 delayed rectifier potassium channel exhibits high-level expression in both principal and inhibitory neurons throughout the central nervous system, including prominent expression in hippocampal neurons. Studies of in vitro preparations suggest that Kv2.1 is a key yet conditional regulator of intrinsic neuronal excitability, mediated by changes in Kv2.1 expression, localization and function via activity-dependent regulation of Kv2.1 phosphorylation. Here we identify neurological and behavioral deficits in mutant (Kv2.1−/−) mice lacking this channel. Kv2.1−/− mice have grossly normal characteristics. No impairment in vision or motor coordination was apparent, although Kv2.1−/− mice exhibit reduced body weight. The anatomic structure and expression of related Kv channels in the brains of Kv2.1−/− mice appears unchanged. Delayed rectifier potassium current is diminished in hippocampal neurons cultured from Kv2.1−/− animals. Field recordings from hippocampal slices of Kv2.1−/− mice reveal hyperexcitability in response to the convulsant bicuculline, and epileptiform activity in response to stimulation. In Kv2.1−/− mice, long-term potentiation at the Schaffer collateral – CA1 synapse is decreased. Kv2.1−/− mice are strikingly hyperactive, and exhibit defects in spatial learning, failing to improve performance in a Morris Water Maze task. Kv2.1−/− mice are hypersensitive to the effects of the convulsants flurothyl and pilocarpine, consistent with a role for Kv2.1 as a conditional suppressor of neuronal activity. Although not prone to spontaneous seizures, Kv2.1−/− mice exhibit accelerated seizure progression. Together, these findings suggest homeostatic suppression of elevated neuronal activity by Kv2.1 plays a central role in regulating neuronal network function. PMID:24494598

  7. The intensive DT neutron generator of TU Dresden

    Directory of Open Access Journals (Sweden)

    Klix Axel

    2018-01-01

    Full Text Available TU Dresden operates an accelerator-based intensive DT neutron generator. Experimental activities comprise investigation into material activation and decay, neutron and photon transport in matter and R&D work on radiation detectors for harsh environments. The intense DT neutron generator is capable to produce a maximum of 1012 n/s. The neutron source is a solid-type water-cooled tritium target based on a titanium matrix on a copper carrier. The neutron yield at a typical deuteron beam current of 1 mA is of the order of 1011 n/s in 4Π. A pneumatic sample transport system is available for short-time irradiations and connected to wo high-purity germanium detector spectrometers for the measurement of induced activities. The overall design of the experimental hall with the neutron generator allows a flexible setup of experiments including the possibility of investigating larger structures and cooled samples or samples at high temperatures.

  8. Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.

    Science.gov (United States)

    Defeu Soufo, Hervé Joël; Reimold, Christian; Linne, Uwe; Knust, Tobias; Gescher, Johannes; Graumann, Peter L

    2010-02-16

    We show that translation initiation factor EF-Tu plays a second important role in cell shape maintenance in the bacterium Bacillus subtilis. EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal element setting up rod cell shape. The localization of MreB and of EF-Tu is interdependent, but in contrast to the dynamic MreB filaments, EF-Tu structures are more static and may serve as tracks for MreB filaments. In agreement with this idea, EF-Tu and MreB interact in vivo and in vitro. Lowering of the EF-Tu levels had a minor effect on translation but a strong effect on cell shape and on the localization of MreB, and blocking of the function of EF-Tu in translation did not interfere with the localization of MreB, showing that, directly or indirectly, EF-Tu affects the cytoskeletal MreB structure and thus serves two important functions in a bacterium.

  9. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    Directory of Open Access Journals (Sweden)

    Nguyen-the Christophe

    2008-10-01

    Full Text Available Abstract Background Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated. Results Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was slightly lower than that of the parental strain. Conclusion The YvfTU two-component system is not required for the expression of most of the virulence factors belonging to the PlcR regulon. However, YvfTU is involved in expression of plcR, a major regulator of virulence in B. cereus.

  10. 14C SIRI samples at CNA: Measurements at 200 kV and 1000 kV

    International Nuclear Information System (INIS)

    Santos Arévalo, Francisco-Javier; Gómez Martínez, Isabel; Agulló García, Lidia

    2015-01-01

    The Sixth International Radiocarbon Intercomparison (SIRI) exercise has taken place during late 2013 and 2014. 13 samples were distributed for AMS (Accelerator Mass Spectrometry) and 5 for radiometric laboratories, including one sample exclusively for radiometric laboratories. Being the first opportunity for our laboratory to participate actively in an intercomparison exercise, we have prepared and measured the samples in the two existing AMS dedicated facilities at the Centro Nacional de Aceleradores (CNA): SARA (Spanish Accelerator for Radionuclide Analysis), a 1 MV multielemental AMS system from HVEE, and Micadas, a 200 kV radiocarbon dating system designed by ETH. Results are presented for the two systems, together with a description of both the sample preparation and measurement procedures.

  11. {sup 14}C SIRI samples at CNA: Measurements at 200 kV and 1000 kV

    Energy Technology Data Exchange (ETDEWEB)

    Santos Arévalo, Francisco-Javier, E-mail: fj.santos@csic.es; Gómez Martínez, Isabel; Agulló García, Lidia

    2015-10-15

    The Sixth International Radiocarbon Intercomparison (SIRI) exercise has taken place during late 2013 and 2014. 13 samples were distributed for AMS (Accelerator Mass Spectrometry) and 5 for radiometric laboratories, including one sample exclusively for radiometric laboratories. Being the first opportunity for our laboratory to participate actively in an intercomparison exercise, we have prepared and measured the samples in the two existing AMS dedicated facilities at the Centro Nacional de Aceleradores (CNA): SARA (Spanish Accelerator for Radionuclide Analysis), a 1 MV multielemental AMS system from HVEE, and Micadas, a 200 kV radiocarbon dating system designed by ETH. Results are presented for the two systems, together with a description of both the sample preparation and measurement procedures.

  12. 14C SIRI samples at CNA: Measurements at 200 kV and 1000 kV

    Science.gov (United States)

    Santos Arévalo, Francisco-Javier; Gómez Martínez, Isabel; Agulló García, Lidia

    2015-10-01

    The Sixth International Radiocarbon Intercomparison (SIRI) exercise has taken place during late 2013 and 2014. 13 samples were distributed for AMS (Accelerator Mass Spectrometry) and 5 for radiometric laboratories, including one sample exclusively for radiometric laboratories. Being the first opportunity for our laboratory to participate actively in an intercomparison exercise, we have prepared and measured the samples in the two existing AMS dedicated facilities at the Centro Nacional de Aceleradores (CNA): SARA (Spanish Accelerator for Radionuclide Analysis), a 1 MV multielemental AMS system from HVEE, and Micadas, a 200 kV radiocarbon dating system designed by ETH. Results are presented for the two systems, together with a description of both the sample preparation and measurement procedures.

  13. Fundamental role for the KCNE4 ancillary subunit in Kv7.4 regulation of arterial tone

    DEFF Research Database (Denmark)

    Jepps, Thomas A; Carr, Georgina; Lundegaard, Pia R

    2015-01-01

    tone by Kv7 channels. In HEK cells expressing Kv7.4, co-expression of KCNE4 increased the membrane expression of Kv7.4 and significantly altered Kv7.4 current properties. Quantitative PCR analysis of different rat arteries found that the KCNE4 isoform predominated and proximity ligation experiments...... leads to reduced Kv7.4 membrane abundance, a depolarized membrane potential and an augmented response to vasoconstrictors. KCNE4 is a key regulator of the function and expression of Kv7.4 in vascular smooth muscle. ABSTRACT: The KCNE ancillary subunits (KCNE1-5) significantly alter the expression...... to reduced Kv7.4 membrane abundance, a depolarized membrane potential and an augmented response to vasoconstrictors. The present study is the first to demonstrate an integral role of KCNE4 in regulating the function and expression of Kv7.4 in vascular smooth muscle....

  14. Harmonization of sound insulation descriptors and classification schemes in Europe: COST Action TU0901

    DEFF Research Database (Denmark)

    Rasmussen, Birgit

    -in-Chief. Handbook of noise and vibration control, USA: Wiley and Son; 2007 [Ch. 114]. [4] COST Action TU0901 “Integrating and Harmonizing Sound Insulation Aspects in Sustainable Urban Housing Constructions”, 2009-2013, www.cost.eu/index.php?id=240&action_number=tu0901 (public information at COST website) or http...... insulation requirements seems unrealistic. However, by preparing a harmonized European classification scheme with a number of quality classes, member states could select a "harmonized" class fitting the national needs and conditions. A joint European Action, COST Action TU0901 "Integrating and Harmonizing...... on good workmanship. The paper will summarize the background, discuss the present situation in Europe and describe the joint efforts to reduce the diversity in Europe, thus supporting and initiating – where needed – improvement of sound insulation of new and existing dwellings in Europe to the benefit...

  15. Ar cinku aizvietotu hidroksilapatītu pētījumi

    OpenAIRE

    Komarovska, Laura

    2013-01-01

    Darbā apkopota informācija par hidroksilapatītu īpašībām, to sintēzes iespējām un izmantošanu. Apkopota informācija arī par izmantotajām darba un analīzes metodēm. Eksperimentālajā daļā veikta ar cinku, cēriju un cinku - cēriju aizvietotu hidroksilapatītu sintēze, izmantojot hidrotermisko metodi, kā sākotnējo izejvielu izmantojot amorfas vai kristāliskas fāzes hidroksilapatītu. Iegūtie pulveri raksturoti izmantojot pulvera rentgendifraktometriju un Furjē transformācijas infrasarkano spektrosk...

  16. Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA

    DEFF Research Database (Denmark)

    Wiborg, Ove; Andersen, C; Knudsen, Charlotte Rohde

    1996-01-01

    Two residues of Escherichia coli elongation factor Tu involved in binding of aminoacyl-tRNA were identified and subjected to mutational analysis. Lys-89 and Asn-90 were each replaced by either Ala or Glu. The four single mutants were denoted K89A, K89E, N90A, and N90E, respectively. The mutants...... were characterized with respect to thermal and chemical stability, GTPase activity, tRNA affinity, and activity in an in vitro translation assay. Most conspicuously tRNA affinities were reduced for all mutants. The results verify our structural analysis of elongation factor Tu in complex with aminoacyl....... Their functional roles are discussed in relation to the structure of elongation factor Tu in complex with aminoacyl-tRNA. Udgivelsesdato: 1996-Aug-23...

  17. The Pandora multi-algorithm approach to automated pattern recognition of cosmic-ray muon and neutrino events in the MicroBooNE detector

    Energy Technology Data Exchange (ETDEWEB)

    Acciarri, R.; Bagby, L.; Baller, B.; Carls, B.; Castillo Fernandez, R.; Cavanna, F.; Greenlee, H.; James, C.; Jostlein, H.; Ketchum, W.; Kirby, M.; Kobilarcik, T.; Lockwitz, S.; Lundberg, B.; Marchionni, A.; Moore, C.D.; Palamara, O.; Pavlovic, Z.; Raaf, J.L.; Schukraft, A.; Snider, E.L.; Spentzouris, P.; Strauss, T.; Toups, M.; Wolbers, S.; Yang, T.; Zeller, G.P. [Fermi National Accelerator Laboratory (FNAL), Batavia, IL (United States); Adams, C. [Harvard University, Cambridge, MA (United States); Yale University, New Haven, CT (United States); An, R.; Littlejohn, B.R.; Martinez Caicedo, D.A. [Illinois Institute of Technology (IIT), Chicago, IL (United States); Anthony, J.; Escudero Sanchez, L.; De Vries, J.J.; Marshall, J.; Smith, A.; Thomson, M. [University of Cambridge, Cambridge (United Kingdom); Asaadi, J. [University of Texas, Arlington, TX (United States); Auger, M.; Ereditato, A.; Goeldi, D.; Kreslo, I.; Lorca, D.; Luethi, M.; Rudolf von Rohr, C.; Sinclair, J.; Weber, M. [Universitaet Bern, Bern (Switzerland); Balasubramanian, S.; Fleming, B.T.; Gramellini, E.; Hackenburg, A.; Luo, X.; Russell, B.; Tufanli, S. [Yale University, New Haven, CT (United States); Barnes, C.; Mousseau, J.; Spitz, J. [University of Michigan, Ann Arbor, MI (United States); Barr, G.; Bass, M.; Del Tutto, M.; Laube, A.; Soleti, S.R.; De Pontseele, W.V. [University of Oxford, Oxford (United Kingdom); Bay, F. [TUBITAK Space Technologies Research Institute, Ankara (Turkey); Bishai, M.; Chen, H.; Joshi, J.; Kirby, B.; Li, Y.; Mooney, M.; Qian, X.; Viren, B.; Zhang, C. [Brookhaven National Laboratory (BNL), Upton, NY (United States); Blake, A.; Devitt, D.; Lister, A.; Nowak, J. [Lancaster University, Lancaster (United Kingdom); Bolton, T.; Horton-Smith, G.; Meddage, V.; Rafique, A. [Kansas State University (KSU), Manhattan, KS (United States); Camilleri, L.; Caratelli, D.; Crespo-Anadon, J.I.; Fadeeva, A.A.; Genty, V.; Kaleko, D.; Seligman, W.; Shaevitz, M.H. [Columbia University, New York, NY (United States); Church, E. [Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Cianci, D.; Karagiorgi, G. [Columbia University, New York, NY (United States); The University of Manchester (United Kingdom); Cohen, E.; Piasetzky, E. [Tel Aviv University, Tel Aviv (Israel); Collin, G.H.; Conrad, J.M.; Hen, O.; Hourlier, A.; Moon, J.; Wongjirad, T.; Yates, L. [Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); Convery, M.; Eberly, B.; Rochester, L.; Tsai, Y.T.; Usher, T. [SLAC National Accelerator Laboratory, Menlo Park, CA (United States); Dytman, S.; Graf, N.; Jiang, L.; Naples, D.; Paolone, V.; Wickremasinghe, D.A. [University of Pittsburgh, Pittsburgh, PA (United States); Esquivel, J.; Hamilton, P.; Pulliam, G.; Soderberg, M. [Syracuse University, Syracuse, NY (United States); Foreman, W.; Ho, J.; Schmitz, D.W.; Zennamo, J. [University of Chicago, IL (United States); Furmanski, A.P.; Garcia-Gamez, D.; Hewes, J.; Hill, C.; Murrells, R.; Porzio, D.; Soeldner-Rembold, S.; Szelc, A.M. [The University of Manchester (United Kingdom); Garvey, G.T.; Huang, E.C.; Louis, W.C.; Mills, G.B.; De Water, R.G.V. [Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Gollapinni, S. [Kansas State University (KSU), Manhattan, KS (United States); University of Tennessee, Knoxville, TN (United States); and others

    2018-01-15

    The development and operation of liquid-argon time-projection chambers for neutrino physics has created a need for new approaches to pattern recognition in order to fully exploit the imaging capabilities offered by this technology. Whereas the human brain can excel at identifying features in the recorded events, it is a significant challenge to develop an automated, algorithmic solution. The Pandora Software Development Kit provides functionality to aid the design and implementation of pattern-recognition algorithms. It promotes the use of a multi-algorithm approach to pattern recognition, in which individual algorithms each address a specific task in a particular topology. Many tens of algorithms then carefully build up a picture of the event and, together, provide a robust automated pattern-recognition solution. This paper describes details of the chain of over one hundred Pandora algorithms and tools used to reconstruct cosmic-ray muon and neutrino events in the MicroBooNE detector. Metrics that assess the current pattern-recognition performance are presented for simulated MicroBooNE events, using a selection of final-state event topologies. (orig.)

  18. The Pandora multi-algorithm approach to automated pattern recognition of cosmic-ray muon and neutrino events in the MicroBooNE detector

    Science.gov (United States)

    Acciarri, R.; Adams, C.; An, R.; Anthony, J.; Asaadi, J.; Auger, M.; Bagby, L.; Balasubramanian, S.; Baller, B.; Barnes, C.; Barr, G.; Bass, M.; Bay, F.; Bishai, M.; Blake, A.; Bolton, T.; Camilleri, L.; Caratelli, D.; Carls, B.; Castillo Fernandez, R.; Cavanna, F.; Chen, H.; Church, E.; Cianci, D.; Cohen, E.; Collin, G. H.; Conrad, J. M.; Convery, M.; Crespo-Anadón, J. I.; Del Tutto, M.; Devitt, D.; Dytman, S.; Eberly, B.; Ereditato, A.; Escudero Sanchez, L.; Esquivel, J.; Fadeeva, A. A.; Fleming, B. T.; Foreman, W.; Furmanski, A. P.; Garcia-Gamez, D.; Garvey, G. T.; Genty, V.; Goeldi, D.; Gollapinni, S.; Graf, N.; Gramellini, E.; Greenlee, H.; Grosso, R.; Guenette, R.; Hackenburg, A.; Hamilton, P.; Hen, O.; Hewes, J.; Hill, C.; Ho, J.; Horton-Smith, G.; Hourlier, A.; Huang, E.-C.; James, C.; Jan de Vries, J.; Jen, C.-M.; Jiang, L.; Johnson, R. A.; Joshi, J.; Jostlein, H.; Kaleko, D.; Karagiorgi, G.; Ketchum, W.; Kirby, B.; Kirby, M.; Kobilarcik, T.; Kreslo, I.; Laube, A.; Li, Y.; Lister, A.; Littlejohn, B. R.; Lockwitz, S.; Lorca, D.; Louis, W. C.; Luethi, M.; Lundberg, B.; Luo, X.; Marchionni, A.; Mariani, C.; Marshall, J.; Martinez Caicedo, D. A.; Meddage, V.; Miceli, T.; Mills, G. B.; Moon, J.; Mooney, M.; Moore, C. D.; Mousseau, J.; Murrells, R.; Naples, D.; Nienaber, P.; Nowak, J.; Palamara, O.; Paolone, V.; Papavassiliou, V.; Pate, S. F.; Pavlovic, Z.; Piasetzky, E.; Porzio, D.; Pulliam, G.; Qian, X.; Raaf, J. L.; Rafique, A.; Rochester, L.; Rudolf von Rohr, C.; Russell, B.; Schmitz, D. W.; Schukraft, A.; Seligman, W.; Shaevitz, M. H.; Sinclair, J.; Smith, A.; Snider, E. L.; Soderberg, M.; Söldner-Rembold, S.; Soleti, S. R.; Spentzouris, P.; Spitz, J.; St. John, J.; Strauss, T.; Szelc, A. M.; Tagg, N.; Terao, K.; Thomson, M.; Toups, M.; Tsai, Y.-T.; Tufanli, S.; Usher, T.; Van De Pontseele, W.; Van de Water, R. G.; Viren, B.; Weber, M.; Wickremasinghe, D. A.; Wolbers, S.; Wongjirad, T.; Woodruff, K.; Yang, T.; Yates, L.; Zeller, G. P.; Zennamo, J.; Zhang, C.

    2018-01-01

    The development and operation of liquid-argon time-projection chambers for neutrino physics has created a need for new approaches to pattern recognition in order to fully exploit the imaging capabilities offered by this technology. Whereas the human brain can excel at identifying features in the recorded events, it is a significant challenge to develop an automated, algorithmic solution. The Pandora Software Development Kit provides functionality to aid the design and implementation of pattern-recognition algorithms. It promotes the use of a multi-algorithm approach to pattern recognition, in which individual algorithms each address a specific task in a particular topology. Many tens of algorithms then carefully build up a picture of the event and, together, provide a robust automated pattern-recognition solution. This paper describes details of the chain of over one hundred Pandora algorithms and tools used to reconstruct cosmic-ray muon and neutrino events in the MicroBooNE detector. Metrics that assess the current pattern-recognition performance are presented for simulated MicroBooNE events, using a selection of final-state event topologies.

  19. Mitochondrial ultrastructure and glucose signaling pathways attributed to the Kv1.3 ion channel

    Directory of Open Access Journals (Sweden)

    Christopher P. Kovach

    2016-05-01

    Full Text Available Gene-targeted deletion of the potassium channel Kv1.3 (Kv1.3-/- results in ‘Super-smeller’ mice with a sensory phenotype that includes an increased olfactory ability linked to changes in olfactory circuitry, increased abundance of olfactory cilia, and increased expression of odorant receptors and the G-protein, Golf. Kv1.3-/- mice also have a metabolic phenotype including lower body weight and decreased adiposity, increased total energy expenditure (TEE, increased locomotor activity, and resistance to both diet- and genetic-induced obesity. We explored two cellular aspects to elucidate the mechanism by which loss of Kv1.3 channel in the olfactory bulb (OB may enhance glucose utilization and metabolic rate. First, using in situ hybridization we find that Kv1.3 and the insulin-dependent glucose transporter type 4 (GLUT4 is co-localized to the mitral cell layer of the OB. Disruption of Kv1.3 conduction via construction of a pore mutation (W386F Kv1.3 was sufficient to independently translocate GLUT4 to the plasma membrane in HEK 293 cells. Because olfactory sensory perception and the maintenance of action potential firing frequency by mitral cells of the OB is highly energy demanding and Kv1.3 is also expressed in mitochondria, we next explored the structure of this organelle in mitral cells. We challenged wildtype (WT and Kv1.3-/- male mice with a moderately high-fat diet (MHF, 31.8 % kcal fat for 4 months and then examined OB ultrastructure using transmission microscopy. In WT mice, mitochondria were significantly enlarged following diet-induced obesity (DIO and there were fewer mitochondria, likely due to mitophagy. Interestingly, mitochondria were significantly smaller in Kv1.3-/- mice compared with that of WT mice. Similar to their metabolic resistance to DIO, the Kv1.3-/- mice had unchanged mitochondria in terms of cross sectional area and abundance following a challenge with modified diet. We are very interested to understand how

  20. Modeling-independent elucidation of inactivation pathways in recombinant and native A-type Kv channels

    Science.gov (United States)

    Fineberg, Jeffrey D.; Ritter, David M.

    2012-01-01

    A-type voltage-gated K+ (Kv) channels self-regulate their activity by inactivating directly from the open state (open-state inactivation [OSI]) or by inactivating before they open (closed-state inactivation [CSI]). To determine the inactivation pathways, it is often necessary to apply several pulse protocols, pore blockers, single-channel recording, and kinetic modeling. However, intrinsic hurdles may preclude the standardized application of these methods. Here, we implemented a simple method inspired by earlier studies of Na+ channels to analyze macroscopic inactivation and conclusively deduce the pathways of inactivation of recombinant and native A-type Kv channels. We investigated two distinct A-type Kv channels expressed heterologously (Kv3.4 and Kv4.2 with accessory subunits) and their native counterparts in dorsal root ganglion and cerebellar granule neurons. This approach applies two conventional pulse protocols to examine inactivation induced by (a) a simple step (single-pulse inactivation) and (b) a conditioning step (double-pulse inactivation). Consistent with OSI, the rate of Kv3.4 inactivation (i.e., the negative first derivative of double-pulse inactivation) precisely superimposes on the profile of the Kv3.4 current evoked by a single pulse because the channels must open to inactivate. In contrast, the rate of Kv4.2 inactivation is asynchronous, already changing at earlier times relative to the profile of the Kv4.2 current evoked by a single pulse. Thus, Kv4.2 inactivation occurs uncoupled from channel opening, indicating CSI. Furthermore, the inactivation time constant versus voltage relation of Kv3.4 decreases monotonically with depolarization and levels off, whereas that of Kv4.2 exhibits a J-shape profile. We also manipulated the inactivation phenotype by changing the subunit composition and show how CSI and CSI combined with OSI might affect spiking properties in a full computational model of the hippocampal CA1 neuron. This work unambiguously

  1. SU-E-I-23: A General KV Constrained Optimization of CNR for CT Abdominal Imaging

    International Nuclear Information System (INIS)

    Weir, V; Zhang, J

    2015-01-01

    Purpose: While Tube current modulation has been well accepted for CT dose reduction, kV adjusting in clinical settings is still at its early stage. This is mainly due to the limited kV options of most current CT scanners. kV adjusting can potentially reduce radiation dose and optimize image quality. This study is to optimize CT abdomen imaging acquisition based on the assumption of a continuous kV, with the goal to provide the best contrast to noise ratio (CNR). Methods: For a given dose (CTDIvol) level, the CNRs at different kV and pitches were measured with an ACR GAMMEX phantom. The phantom was scanned in a Siemens Sensation 64 scanner and a GE VCT 64 scanner. A constrained mathematical optimization was used to find the kV which led to the highest CNR for the anatomy and pitch setting. Parametric equations were obtained from polynomial fitting of plots of kVs vs CNRs. A suitable constraint region for optimization was chosen. Subsequent optimization yielded a peak CNR at a particular kV for different collimations and pitch setting. Results: The constrained mathematical optimization approach yields kV of 114.83 and 113.46, with CNRs of 1.27 and 1.11 at the pitch of 1.2 and 1.4, respectively, for the Siemens Sensation 64 scanner with the collimation of 32 x 0.625mm. An optimized kV of 134.25 and 1.51 CNR is obtained for a GE VCT 64 slice scanner with a collimation of 32 x 0.625mm and a pitch of 0.969. At 0.516 pitch and 32 x 0.625 mm an optimized kV of 133.75 and a CNR of 1.14 was found for the GE VCT 64 slice scanner. Conclusion: CNR in CT image acquisition can be further optimized with a continuous kV option instead of current discrete or fixed kV settings. A continuous kV option is a key for individualized CT protocols

  2. SU-E-I-23: A General KV Constrained Optimization of CNR for CT Abdominal Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Weir, V; Zhang, J [University of Kentucky, Lexington, KY (United States)

    2015-06-15

    Purpose: While Tube current modulation has been well accepted for CT dose reduction, kV adjusting in clinical settings is still at its early stage. This is mainly due to the limited kV options of most current CT scanners. kV adjusting can potentially reduce radiation dose and optimize image quality. This study is to optimize CT abdomen imaging acquisition based on the assumption of a continuous kV, with the goal to provide the best contrast to noise ratio (CNR). Methods: For a given dose (CTDIvol) level, the CNRs at different kV and pitches were measured with an ACR GAMMEX phantom. The phantom was scanned in a Siemens Sensation 64 scanner and a GE VCT 64 scanner. A constrained mathematical optimization was used to find the kV which led to the highest CNR for the anatomy and pitch setting. Parametric equations were obtained from polynomial fitting of plots of kVs vs CNRs. A suitable constraint region for optimization was chosen. Subsequent optimization yielded a peak CNR at a particular kV for different collimations and pitch setting. Results: The constrained mathematical optimization approach yields kV of 114.83 and 113.46, with CNRs of 1.27 and 1.11 at the pitch of 1.2 and 1.4, respectively, for the Siemens Sensation 64 scanner with the collimation of 32 x 0.625mm. An optimized kV of 134.25 and 1.51 CNR is obtained for a GE VCT 64 slice scanner with a collimation of 32 x 0.625mm and a pitch of 0.969. At 0.516 pitch and 32 x 0.625 mm an optimized kV of 133.75 and a CNR of 1.14 was found for the GE VCT 64 slice scanner. Conclusion: CNR in CT image acquisition can be further optimized with a continuous kV option instead of current discrete or fixed kV settings. A continuous kV option is a key for individualized CT protocols.

  3. Towards an understanding of structure-function relationships of elongation factor Tu

    DEFF Research Database (Denmark)

    Wiborg, O; Andersen, C; Knudsen, Charlotte Rohde

    1994-01-01

    In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point-mutated. Prel......In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point...

  4. Functional and Molecular Evidence for Kv7 Channel Subtypes in Human Detrusor from Patients with and without Bladder Outflow Obstruction

    DEFF Research Database (Denmark)

    Svalø, Julie; Sheykhzade, Majid; Nordling, Jørgen

    2015-01-01

    The aim of the study was to investigate whether Kv7 channels and their ancillary β-subunits, KCNE, are functionally expressed in the human urinary bladder. Kv7 channels were examined at the molecular level and by functional studies using RT-qPCR and myography, respectively. We found mRNA expressi...... between Kv7 channels and β-adrenoceptors in the human urinary bladder. The performed gene expression analysis combined with the organ bath studies imply that compounds that activate Kv7 channels could be useful for treatment of overactive bladder syndrome.......The aim of the study was to investigate whether Kv7 channels and their ancillary β-subunits, KCNE, are functionally expressed in the human urinary bladder. Kv7 channels were examined at the molecular level and by functional studies using RT-qPCR and myography, respectively. We found mRNA expression...... (activator of Kv7.1 channels, 10 μM) and ML213 (activator of Kv7.2, Kv7.4, Kv7.4/7.5 and Kv7.5 channels, 10 μM), reduced the tone of 1 μM carbachol pre-constricted bladder strips. XE991 (blocker of Kv7.1-7.5 channels, 10 μM) had opposing effects as it increased contractions achieved with 20 mM KPSS...

  5. KCNE1 constrains the voltage sensor of Kv7.1 K+ channels.

    Directory of Open Access Journals (Sweden)

    Liora Shamgar

    Full Text Available Kv7 potassium channels whose mutations cause cardiovascular and neurological disorders are members of the superfamily of voltage-gated K(+ channels, comprising a central pore enclosed by four voltage-sensing domains (VSDs and sharing a homologous S4 sensor sequence. The Kv7.1 pore-forming subunit can interact with various KCNE auxiliary subunits to form K(+ channels with very different gating behaviors. In an attempt to characterize the nature of the promiscuous gating of Kv7.1 channels, we performed a tryptophan-scanning mutagenesis of the S4 sensor and analyzed the mutation-induced perturbations in gating free energy. Perturbing the gating energetics of Kv7.1 bias most of the mutant channels towards the closed state, while fewer mutations stabilize the open state or the inactivated state. In the absence of auxiliary subunits, mutations of specific S4 residues mimic the gating phenotypes produced by co-assembly of Kv7.1 with either KCNE1 or KCNE3. Many S4 perturbations compromise the ability of KCNE1 to properly regulate Kv7.1 channel gating. The tryptophan-induced packing perturbations and cysteine engineering studies in S4 suggest that KCNE1 lodges at the inter-VSD S4-S1 interface between two adjacent subunits, a strategic location to exert its striking action on Kv7.1 gating functions.

  6. The SafeBoosC Phase II Randomised Clinical Trial : A Treatment Guideline for Targeted Near-Infrared-Derived Cerebral Tissue Oxygenation versus Standard Treatment in Extremely Preterm Infants

    NARCIS (Netherlands)

    Pellicer, Adelina; Greisen, Gorm; Benders, Manon; Claris, Olivier; Dempsey, Eugene; Fumagalli, Monica; Gluud, Christian; Hagmann, Cornelia; Hellstroem-Westas, Lena; Hyttel-Sorensen, Simon; Lemmers, Petra; Naulaers, Gunnar; Pichler, Gerhard; Roll, Claudia; van Bel, Frank; van Oeveren, Wim; Skoog, Maria; Wolf, Martin; Austin, Topun

    2013-01-01

    Near-infrared spectroscopy-derived regional tissue oxygen saturation of haemoglobin (rSto(2)) reflects venous oxygen saturation. If cerebral metabolism is stable, rSto(2) can be used as an estimate of cerebral oxygen delivery. The SafeBoosC phase II randomised clinical trial hypothesises that the

  7. The secret life of ion channels: Kv1.3 potassium channels and proliferation.

    Science.gov (United States)

    Pérez-García, M Teresa; Cidad, Pilar; López-López, José R

    2018-01-01

    Kv1.3 channels are involved in the switch to proliferation of normally quiescent cells, being implicated in the control of cell cycle in many different cell types and in many different ways. They modulate membrane potential controlling K + fluxes, sense changes in potential, and interact with many signaling molecules through their intracellular domains. From a mechanistic point of view, we can describe the role of Kv1.3 channels in proliferation with at least three different models. In the "membrane potential model," membrane hyperpolarization resulting from Kv1.3 activation provides the driving force for Ca 2+ influx required to activate Ca 2+ -dependent transcription. This model explains most of the data obtained from several cells from the immune system. In the "voltage sensor model," Kv1.3 channels serve mainly as sensors that transduce electrical signals into biochemical cascades, independently of their effect on membrane potential. Kv1.3-dependent proliferation of vascular smooth muscle cells (VSMCs) could fit this model. Finally, in the "channelosome balance model," the master switch determining proliferation may be related to the control of the Kv1.3 to Kv1.5 ratio, as described in glial cells and also in VSMCs. Since the three mechanisms cannot function independently, these models are obviously not exclusive. Nevertheless, they could be exploited differentially in different cells and tissues. This large functional flexibility of Kv1.3 channels surely gives a new perspective on their functions beyond their elementary role as ion channels, although a conclusive picture of the mechanisms involved in Kv1.3 signaling to proliferation is yet to be reached.

  8. X irradiation of human epidermis in vitro. 2. Comparison of single 44 kV and 200 kV X irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Wollina, U; Fueller, J; Burger, B; Hipler, C

    1989-01-01

    On the example of the reduction of epidermal adhesion of FITC wheat germ agglutinine (WGA) the direct membrane effect of a single X irradiation (44 kV and 220 kV) was analyzed in vitro. Human normal skin and psoriasis centres were compared. Normal skin showed no alteration of microscopically visible FITC-WGA adhesion on epidermal cells over the whole dose range. Foci of psoriasis responded to doses of /ge/ 5 Gy (44 and 220 kV) with a drastic reduction of epidermal lectin binding to lower and medium cell layers. Maximum efficacy was with 5 Gy (44 kV) or 10 Gy (220 kV). A dose elevation up to 20 Gy did not result in an increase of efficacy. Topographically the radiosensitive FITC-WGA adhesion could chiefly be seen in the dermal ridges. The findings support the impression of an increased radiosensitivity of the lesional psoriatic epidermis compared with normal skin. This is connected with an abnormal differentiation of keratinocytes in psoriasis. (author).

  9. Development and use of 60 kV, and 150 kV floating deck modulators for high voltage protection of multi-megawatt ion beam accelerators

    International Nuclear Information System (INIS)

    Barber, G.C.; Ponte, N.S.; Schilling, G.

    1977-01-01

    Extraction currents of 60 A at 40 kV have been produced by utilizing a 60 kV floating deck modulator interfaced to a high voltage power supply. The modulator is operated in a series mode to repetitively pulse power to the ion beam accelerator. Current monitoring and other protective circuits provide interrupt commands to the series switch tube when faults occur. The constant current characteristics of the water cooled tetrode and the rapid response of the protective circuits effectively limit the fault energy to the ion source. Three of the 60 kV decks have been modified and stacked in a series configuration to supply 150 kV, 50 A pulses. This system supplies power for development of higher-energy multi-grid sources. In this system attention has been focused on forced voltage sharing of the three decks and on protective circuits for fault conditions. All control signal processing and conditioning is performed at ground level. Fiber optic links are used to interface with the high potential associated with the floating decks. A shunt modulator incorporated with this system provides regulation of the voltage to the ion source gradient grid. Future modulator development includes a system to deliver 100 A at 80 kV

  10. Development of 600 kV triple resonance pulse transformer.

    Science.gov (United States)

    Li, Mingjia; Zhang, Faqiang; Liang, Chuan; Xu, Zhou

    2015-06-01

    In this paper, a triple-resonance pulse transformer based on an air-core transformer is introduced. The voltage across the high-voltage winding of the air-core transformer is significantly less than the output voltage; instead, the full output voltage appears across the tuning inductor. The maximum ratio of peak load voltage to peak transformer voltage is 2.77 in theory. By analyzing pulse transformer's lossless circuit, the analytical expression for the output voltage and the characteristic equation of the triple-resonance circuit are presented. Design method for the triple-resonance pulse transformer (iterated simulation method) is presented, and a triple-resonance pulse transformer is developed based on the existing air-core transformer. The experimental results indicate that the maximum ratio of peak voltage across the load to peak voltage across the high-voltage winding of the air-core transformer is approximately 2.0 and the peak output voltage of the triple-resonance pulse transformer is approximately 600 kV.

  11. Accurate Iterative Analysis of the K-V Equations

    CERN Document Server

    Anderson, Oscar A

    2005-01-01

    Previous solutions of the K-V equations have either yielded poor accuracy or have been complex and difficult to follow. We describe a new approach, simple in concept, easy to use, with accuracy substantially improved over previous treatments. The results are given in the same form as the smooth approximation but include a few correction terms obtained from the field gradient integrated along the axis of a quadrupole cell. The input quantities–quadrupole field, beam current, and emittance–yield the average beam radius, the maximum envelope excursion, and the depressed and undepressed tunes. For all values of the input parameters, the results are much closer to the exact values from simulations than are results from the smooth approximation. For example, with the parameters adjusted for an exact phase advance of 83.4 degrees and 50% tune depression, both tunes are in error by less than 0.5%–over 22 times better than the smooth approximation. The error in maximum radius is 0.04%, impro...

  12. Novel Kv7.1-phosphatidylinositol 4,5-bisphosphate interaction sites uncovered by charge neutralization scanning

    DEFF Research Database (Denmark)

    Eckey, Karina; Wrobel, Eva; Strutz-Seebohm, Nathalie

    2014-01-01

    Kv7.1 to Kv7.5 α-subunits belong to the family of voltage-gated potassium channels (Kv). Assembled with the β-subunit KCNE1, Kv7.1 conducts the slowly activating potassium current IKs, which is one of the major currents underlying repolarization of the cardiac action potential. A known regulator...... of corresponding long QT syndrome mutants suggested impaired PIP2 regulation as the cause for channel dysfunction. To clarify the underlying structural mechanism of PIP2 binding, molecular dynamics simulations of Kv7.1/KCNE1 complexes containing two PIP2 molecules in each subunit at specific sites were performed...

  13. An efficient algorithm for nucleolus and prekernel computation in some classes of TU-games

    NARCIS (Netherlands)

    Faigle, U.; Kern, Walter; Kuipers, J.

    1998-01-01

    We consider classes of TU-games. We show that we can efficiently compute an allocation in the intersection of the prekernel and the least core of the game if we can efficiently compute the minimum excess for any given allocation. In the case where the prekernel of the game contains exactly one core

  14. Darboux transformation and soliton solutions for the Boiti-Pempinelli-Tu (BPT) hierarchy

    International Nuclear Information System (INIS)

    Wang Jiong

    2005-01-01

    Starting from a spectral problem, we derive the well-known Boiti-Pempinelli-Tu (BPT) hierarchy. An explicit and universal Darboux transformation for the whole hierarchy is constructed. The soliton solutions for the BPT hierarchy are obtained by applying the Darboux transformation

  15. Comparison of Plasma Tu-M2-PK and CA19-9 in Pancreatic Cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Heegaard, Niels H H; Schaffalitzky de Muckadell, Ove B

    2009-01-01

    because of suspicion of pancreatic cancer. Of these, 51 patients had their conditions diagnosed as PDAC, whereas this diagnosis was ruled out in 52 after 12 months of follow-up. The performance of Tu-M2-PK was compared with that of CA19-9 using cutoff values 15 and 37 U/mL, respectively. RESULTS...

  16. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu.

    Science.gov (United States)

    Castro-Roa, Daniel; Garcia-Pino, Abel; De Gieter, Steven; van Nuland, Nico A J; Loris, Remy; Zenkin, Nikolay

    2013-12-01

    Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic family and inhibits bacterial translation by an unknown mechanism. Here we show that, in contrast to having AMPylating activity, Doc is a new type of kinase that inhibits bacterial translation by phosphorylating the conserved threonine (Thr382) of the translation elongation factor EF-Tu, rendering EF-Tu unable to bind aminoacylated tRNAs. We provide evidence that EF-Tu phosphorylation diverged from AMPylation by antiparallel binding of the NTP relative to the catalytic residues of the conserved Fic catalytic core of Doc. The results bring insights into the mechanism and role of phosphorylation of EF-Tu in bacterial physiology as well as represent an example of the catalytic plasticity of enzymes and a mechanism for the evolution of new enzymatic activities.

  17. Všechno už tu bylo. Jenom trochu menší.

    Czech Academy of Sciences Publication Activity Database

    Mikuláš, Radek

    2016-01-01

    Roč. 95, č. 3 (2016), s. 154-157 ISSN 0042-4544 Institutional support: RVO:67985831 Keywords : geology * geological history of the Earth * historical geology * geological eras * Anthropocene * paleontology * ichnology * fossils * trace fossils * fossil animals * sediments Subject RIV: DB - Geology ; Mineralogy http://casopis.vesmir.cz/clanek/vsechno-uz-tu-bylo

  18. Manûtu ša Bābili = the Babylonian subdivision of the mina

    NARCIS (Netherlands)

    van der Spek, R.J.

    A new interpretation of the term Manûtu ša Bābili is presented here. It is not the exchange rate between shekels and drachmas, as was generally assumed, but it is the Babylonian subdivision ("counting") of the mina as opposed to the Greek mina. A Babylonian mina counts 30 staters, a Greek mina 25

  19. CWI and TU Delft at TREC 2013: Contextual Suggestion, Federated Web Search, KBA, and Web Tracks

    NARCIS (Netherlands)

    A. Bellogín Kouki (Alejandro); G.G. Gebremeskel (Gebre); J. He (Jiyin); J.J.P. Lin (Jimmy); A. Said (Alan); T. Samar (Thaer); A.P. de Vries (Arjen); J.B.P. Vuurens (Jeroen)

    2014-01-01

    htmlabstractThis paper provides an overview of the work done at the Centrum Wiskunde & Informatica (CWI) and Delft University of Technology (TU Delft) for different tracks of TREC 2013. We participated in the Contextual Suggestion Track, the Federated Web Search Track, the Knowledge Base

  20. RESULTS OF AIRPLANE TU-204-300 OPERATED BY "VLADIVOSTOK AVIA" COMPANY FUEL FLOW MONITIRING

    Directory of Open Access Journals (Sweden)

    G. E. Maslennikova

    2014-01-01

    Full Text Available This article describes the results obtained from continuous monitoring of fuel flow on the airplanes Tu-204-300, operated by the aircompany "Vladivostok Avia". The reasons for the change in the cost of each copy of airplane and changes in fuel characteristics due to pilots manner of flying.

  1. Česká literatura a kultura za protektorátu

    Czech Academy of Sciences Publication Activity Database

    Piorecký, Karel

    2017-01-01

    Roč. 65, č. 3 (2017), s. 482-485 ISSN 0009-0468. [Česká literatura a kultura za protektorátu. Ostrava, 24.11.2016-25.11.2016] Institutional support: RVO:68378068 Keywords : Czech literature * 1938-1945 Subject RIV: AJ - Letters, Mass-media, Audiovision

  2. On the Potentials for Synergy Between COST Action TU1406 and the JCSS

    DEFF Research Database (Denmark)

    Nielsen, Michael Havbro Faber

    On of the main challenges of COST Action TU1406 is to identify, categorize and to the extent possible represent performance indicators of roadway bridges in a manner supporting decision making for their service life integrity management. This calls for an information consistent approach accounting...

  3. Protein kinase A stimulates Kv7.1 surface expression by regulating Nedd4-2-dependent endocytic trafficking

    DEFF Research Database (Denmark)

    Andersen, Martin Nybo; Hefting, Louise Leth; Steffensen, Annette Buur

    2015-01-01

    The potassium channel Kv7.1 plays critical physiological roles in both heart and epithelial tissues. In heart, Kv7.1 and the accessory subunit KCNE1 forms the IKs current, which is enhanced by PKA mediated phosphorylation. The observed current increase requires both phosphorylation of Kv7.......1 and the presence of KCNE1. However, PKA also stimulates Kv7.1 currents in epithelial tissues, such as colon, where the channel does not co-assemble with KCNE1. Here, we demonstrate that PKA activity significantly impacts the subcellular localization of Kv7.1 in Madin Darby Canine Kidney cells. While PKA inhibition...... reduced the fraction of channels at the cell surface, PKA activation increased it. We show that PKA inhibition lead to intracellular accumulation of Kv7.1 in late endosomes/lysosomes. By mass spectroscopy we identified eight phosphorylated residues on Kv7.1, however, none appeared to play a role...

  4. Major Refit for CERN's 400 kV Substation (Part of the new control and protection system for the 18 kV side)

    CERN Document Server

    2001-01-01

    The 400 kV substation on the Prévessin site brings in the electricity that powers CERN's accelerators and the majority of the Laboratory's installations. It was originally built in the 1970s for the SPS, and is one of only five privately owned 400 kV sub-stations in France. Three of the others belong to the national railway company, SNCF, supplying the Paris-Marseilles TGV line, the other is at the Cadarache research centre near mouth of the Rhone. After nearly thirty years of service, CERN's substation has just undergone a complete overhaul.

  5. A sub-GeV charged-current quasi-elastic νμ cross-section on carbon at SciBooNE

    International Nuclear Information System (INIS)

    Walding, Joseph James

    2010-01-01

    Neutrino-nucleus charged-current quasi-elastic scattering is the signal interaction used by many neutrino oscillation experiments. For muon disappearance studies the signal mode is ν μ n → μp. Modern oscillation experiments, such as T2K, produce neutrino beams with peak beam energies of order a few-GeV. It is therefore vitally important to have accurate measurements of the charged-current quasi-elastic cross-section for future neutrino oscillation experiments. Neutrino-nucleus cross-sections in the few-GeV region are not well understood, with the main uncertainties coming from understanding of the neutrino beam flux and the final state interactions within nuclei. SciBooNE is a sub-GeV neutrino-nucleus cross-section experiment based at Fermilab, Batavia, USA, with the goal to measure neutrino cross-sections with precision of order 5%. SciBooNE took data from June 2007 until August 2008, in total 0.99 x 10 20 and 1.53 x 10 20 protons on target were collected in neutrino and anti-neutrino mode, respectively. In this thesis a ν μ charged-current quasi-elastic (CCQE) cross-section contained within the SciBar sub-detector is presented. A method to tag muons in SciBar was developed and three samples were isolated. An excess in backwards tracks in the one-track sample is observed. A Poisson maximum likelihood is used to extract the CCQE cross-section. The fit was applied using a basic fit parameter model, successfully used to obtain the cross-section in the SciBar-MRD matched CCQE analysis. This method was found to be insufficient in describing the data for the SciBar-contained CCQE analysis. By adding two migration parameters the cross-section was calculated to be 1.004 ± 0.031 (stat) -0.150 +0.101 (sys) x 10 -38 cm 2 /neutron, excluding backwards tracks with a χ 2 = 203.8/76 d.o.f. and 1.083 ± 0.030(stat) -0.177 +0.115 (sys) x 10 -38 cm 2 /neutron, including backwards tracks with a χ 2 = 659.8/133 d.o.f. Only neutrino beam and detector systematics have been

  6. Regulation of Kv1.4 potassium channels by PKC and AMPK kinases

    DEFF Research Database (Denmark)

    Andersen, Martin Nybo; Skibsbye, Lasse; Saljic, Arnela

    2018-01-01

    around the ubiquitin ligase Nedd4-2. In the present study we examined whether Kv1.4, constituting the cardiac Ito,s current, is subject to similar regulation. In the epithelial Madin-Darby Canine Kidney (MDCK) cell line, which constitutes a highly reproducible model system for addressing membrane...... targeting, we find, by confocal microscopy, that Kv1.4 cell surface expression is downregulated by activation of protein kinase C (PKC) and AMP-activated protein kinase (AMPK). In contrast, manipulating the activities of phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) and serum and glucocorticoid......-regulated kinase 1 (SGK1) were without effect on channel localization. The PKC and AMPK-mediated downregulation of Kv1.4 membrane surface localization was confirmed by two-electrode voltage clamp in Xenopus laevis oocytes, where pharmacological activation of PKC and AMPK reduced Kv1.4 current levels. We further...

  7. Downregulation of Kv7.4 channel activity in primary and secondary hypertension

    DEFF Research Database (Denmark)

    Jepps, Thomas Andrew; Chadha, Preet S; Davis, Alison J

    2011-01-01

    Voltage-gated potassium (K(+)) channels encoded by KCNQ genes (Kv7 channels) have been identified in various rodent and human blood vessels as key regulators of vascular tone; however, nothing is known about the functional impact of these channels in vascular disease. We ascertained the effect of...... structurally different activators of Kv7.2 through Kv7.5 channels (BMS-204352, S-1, and retigabine) on blood vessels from normotensive and hypertensive animals.......Voltage-gated potassium (K(+)) channels encoded by KCNQ genes (Kv7 channels) have been identified in various rodent and human blood vessels as key regulators of vascular tone; however, nothing is known about the functional impact of these channels in vascular disease. We ascertained the effect of 3...

  8. Kv7.1 surface expression is regulated by epithelial cell polarization

    DEFF Research Database (Denmark)

    Andersen, Martin N; Olesen, Søren-Peter; Rasmussen, Hanne Borger

    2011-01-01

    The potassium channel K(V)7.1 is expressed in the heart where it contributes to the repolarization of the cardiac action potential. In addition, K(V)7.1 is expressed in epithelial tissues where it plays a role in salt and water transport. Mutations in the kcnq1 gene can lead to long QT syndrome...... and deafness, and several mutations have been described as trafficking mutations. To learn more about the basic mechanisms that regulate K(V)7.1 surface expression, we have investigated the trafficking of K(V)7.1 during the polarization process of the epithelial cell line Madin-Darby Canine Kidney (MDCK) using...... is regulated by signaling mechanisms involved in epithelial cell polarization in particular signaling cascades involving protein kinase C and PI3K....

  9. A comparison of kV and MV imaging in head and neck image guided radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Devereux, B. [Radiation Oncology Queensland, 280 North St, Toowoomba 4350 (Australia)], E-mail: beth.devereux@roq.net.au; Frantzis, J.; Sisson, T.; Jones, M.; Martin, J.; Middleton, M. [Radiation Oncology Queensland, 280 North St, Toowoomba 4350 (Australia)

    2010-02-15

    Purpose: To compare and assess kV and MV imaging modalities and their role in image guided radiotherapy (IGRT) for head and neck cancer patients. Method: Twelve patients receiving radical radiotherapy to the head and neck were analysed in this study. Six patients undertook MV daily online intervention and a further six patients undertook kV daily online intervention. Pre-intervention field placement data were collected from three separate observers' image match analysis for each patient. The radiotherapy collective involved in the daily online image match analysis formed the fourth observer in the study. The primary end point was to establish the difference in inter- and intra-observer variance between kV and MV imaging modalities. Results: The range of the standard deviations of systematic set-up error for MV imaging calculated was 1.47-2.33 mm (MV) and 1.61-1.64 mm (kV) for the right-left (RL), 2.10-2.17 mm (MV) and 1.53-1.84 mm (kV) for the cranio-caudal (CC) and 1.43-1.63 mm (MV) and 1.02-1.11 mm (kV) for the anterior-posterior (AP). The mean inter-observer variance was 0.21 mm (MV) and 0.41 mm (kV) for the RL, 0.53 mm (MV) and 0.55 mm (kV) for the CC and 0.23 mm (MV) and 0.16 mm (kV) for the AP direction. Intra-observer mean variance was in the order of 0.60 mm (MV) and 0.16 mm (kV) for the RL, 1.41 mm (MV) and 0.05 mm (kV) for the CC and 1.41 mm (MV) and 0.08 mm (kV) for the AP. Discussion: The data in this study suggest both inter- and intra-observer consistency across kV and MV imaging modalities were comparable. However, it is felt that the improved clarity and quality of kV imaging allows all observers to analyse images in a consistent manner, identifying and acting on potential field placement moves. Conclusion: The introduction of kV imaging has maintained the high levels of inter- and intra-observer consistency achieved with MV imaging. This in turn further enables positive verification outcomes and supports the implementation of potential

  10. Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region

    DEFF Research Database (Denmark)

    Mansilla, Francisco; Knudsen, Charlotte Rohde; Laurberg, M

    1998-01-01

    We have mutated lysine 2 and arginine 7 in elongation factor Tu from Escherichia coli separately either to alanine or glutamic acid. The aim of the work was to reveal the possible interactions between the conserved N-terminal part of the molecule, which is rich in basic residues and aminoacyl...... this activity. Furthermore, arginine 7 seems to play a role in regulating the binding of GTP. The three-dimensional structure of the ternary complex, EF-Tu:GTP:Phe-tRNAPhe, involving Thermus aquaticus EF-Tu and yeast Phe-tRNA(Phe), shows that Arg7 is in a position which permits salt bridge formation with Asp284...

  11. The development of a 200 kV monochromated field emission electron source

    Energy Technology Data Exchange (ETDEWEB)

    Mukai, Masaki, E-mail: mmukai@jeol.co.jp [JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558 (Japan); Kim, Judy S. [University of Oxford, Department of Materials, Parks Road, Oxford, OX1 3PH (United Kingdom); Omoto, Kazuya; Sawada, Hidetaka; Kimura, Atsushi; Ikeda, Akihiro; Zhou, Jun; Kaneyama, Toshikatsu [JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558 (Japan); Young, Neil P.; Warner, Jamie H.; Nellist, Peter D.; Kirkland, Angus I. [University of Oxford, Department of Materials, Parks Road, Oxford, OX1 3PH (United Kingdom)

    2014-05-01

    We report the development of a monochromator for an intermediate-voltage aberration-corrected electron microscope suitable for operation in both STEM and TEM imaging modes. The monochromator consists of two Wien filters with a variable energy selecting slit located between them and is located prior to the accelerator. The second filter cancels the energy dispersion produced by the first filter and after energy selection forms a round monochromated, achromatic probe at the specimen plane. The ultimate achievable energy resolution has been measured as 36 meV at 200 kV and 26 meV at 80 kV. High-resolution Annular Dark Field STEM images recorded using a monochromated probe resolve Si–Si spacings of 135.8 pm using energy spreads of 218 meV at 200 kV and 217 meV at 80 kV respectively. In TEM mode an improvement in non-linear spatial resolution to 64 pm due to the reduction in the effects of partial temporal coherence has been demonstrated using broad beam illumination with an energy spread of 134 meV at 200 kV. - Highlights: • Monochromator for 200 kV aberration corrected TEM and STEM was developed. • Monochromator produces monochromated and achromatic probe at specimen plane. • Ultimate energy resolution was measured to be 36 meV at 200 kV and 26 meV at 80 kV. • Atomic resolution STEM images were recorded using monochromated electron probe. • Improvements of TEM resolution were confirmed using monochromated illumination.

  12. Effects of the small molecule HERG activator NS1643 on Kv11.3 channels.

    Directory of Open Access Journals (Sweden)

    Arne Bilet

    Full Text Available NS1643 is one of the small molecule HERG (Kv11.1 channel activators and has also been found to increase erg2 (Kv11.2 currents. We now investigated whether NS1643 is also able to act as an activator of Kv11.3 (erg3 channels expressed in CHO cells. Activation of rat Kv11.3 current occurred in a dose-dependent manner and maximal current increasing effects were obtained with 10 µM NS1643. At this concentration, steady-state outward current increased by about 80% and the current increase was associated with a significant shift in the voltage dependence of activation to more negative potentials by about 15 mV. In addition, activation kinetics were accelerated, whereas deactivation was slowed. There was no significant effect on the kinetics of inactivation and recovery from inactivation. The strong current-activating agonistic effect of NS1643 did not result from a shift in the voltage dependence of Kv11.3 channel inactivation and was independent from external Na(+ or Ca(2+. At the higher concentration of 20 µM, NS1643 induced clearly less current increase. The left shift in the voltage dependence of activation reversed and the voltage sensitivity of activation dramatically decreased along with a slowing of Kv11.3 channel activation. These data show that, in comparison to other Kv11 family members, NS1643 exerts distinct effects on Kv11.3 channels with especially pronounced partial antagonistic effects at higher concentration.

  13. The development of a 200 kV monochromated field emission electron source

    International Nuclear Information System (INIS)

    Mukai, Masaki; Kim, Judy S.; Omoto, Kazuya; Sawada, Hidetaka; Kimura, Atsushi; Ikeda, Akihiro; Zhou, Jun; Kaneyama, Toshikatsu; Young, Neil P.; Warner, Jamie H.; Nellist, Peter D.; Kirkland, Angus I.

    2014-01-01

    We report the development of a monochromator for an intermediate-voltage aberration-corrected electron microscope suitable for operation in both STEM and TEM imaging modes. The monochromator consists of two Wien filters with a variable energy selecting slit located between them and is located prior to the accelerator. The second filter cancels the energy dispersion produced by the first filter and after energy selection forms a round monochromated, achromatic probe at the specimen plane. The ultimate achievable energy resolution has been measured as 36 meV at 200 kV and 26 meV at 80 kV. High-resolution Annular Dark Field STEM images recorded using a monochromated probe resolve Si–Si spacings of 135.8 pm using energy spreads of 218 meV at 200 kV and 217 meV at 80 kV respectively. In TEM mode an improvement in non-linear spatial resolution to 64 pm due to the reduction in the effects of partial temporal coherence has been demonstrated using broad beam illumination with an energy spread of 134 meV at 200 kV. - Highlights: • Monochromator for 200 kV aberration corrected TEM and STEM was developed. • Monochromator produces monochromated and achromatic probe at specimen plane. • Ultimate energy resolution was measured to be 36 meV at 200 kV and 26 meV at 80 kV. • Atomic resolution STEM images were recorded using monochromated electron probe. • Improvements of TEM resolution were confirmed using monochromated illumination

  14. 40-kV, 25-ms neutral-beam power supply for TMX

    International Nuclear Information System (INIS)

    Leavitt, G.A.

    1977-01-01

    Modifications are described to upgrade the neutral-beam power supply for the TMX from 40 kV, 10 ms to 40 kV, 25 ms. The redesign of the accel and suppressor power supplies to achieve separation of the high-voltage and control sections, operation of the arc pulse lines in series, operation of the arc pulse lines in a noisy environment with SCR trigger and crowbar, and modifications to the electrolytic storage banks are discussed

  15. Analisa Penggunaan Recloser untuk Pengaman Arus Lebih pada Jaringan Distribusi 20 Kv Gardu Induk Garuda Sakti

    OpenAIRE

    Putra, Ario; Firdaus, Firdaus

    2017-01-01

    PT.PLN: 20 kV network substations Garuda Sakti, frequently interference, one of interferences is the short-circuit current. To install equipment to include Recloser. This paper discuss the analysisof the use Recloser on distribution network 20 kV, this paper also be simulated using the software ETAP 12.6 which will feature some of the parameters for comparison of calculations and data thatalready exists in PLN, with two parameters, short circuit and coordination Recloser with overcurrent rela...

  16. Nonrandom γ-TuNA-dependent spatial pattern of microtubule nucleation at the Golgi.

    Science.gov (United States)

    Sanders, Anna A W M; Chang, Kevin; Zhu, Xiaodong; Thoppil, Roslin J; Holmes, William R; Kaverina, Irina

    2017-11-07

    Noncentrosomal microtubule (MT) nucleation at the Golgi generates MT network asymmetry in motile vertebrate cells. Investigating the Golgi-derived MT (GDMT) distribution, we find that MT asymmetry arises from nonrandom nucleation sites at the Golgi (hotspots). Using computational simulations, we propose two plausible mechanistic models of GDMT nucleation leading to this phenotype. In the "cooperativity" model, formation of a single GDMT promotes further nucleation at the same site. In the "heterogeneous Golgi" model, MT nucleation is dramatically up-regulated at discrete and sparse locations within the Golgi. While MT clustering in hotspots is equally well described by both models, simulating MT length distributions within the cooperativity model fits the data better. Investigating the molecular mechanism underlying hotspot formation, we have found that hotspots are significantly smaller than a Golgi subdomain positive for scaffolding protein AKAP450, which is thought to recruit GDMT nucleation factors. We have further probed potential roles of known GDMT-promoting molecules, including γ-TuRC-mediated nucleation activator (γ-TuNA) domain-containing proteins and MT stabilizer CLASPs. While both γ-TuNA inhibition and lack of CLASPs resulted in drastically decreased GDMT nucleation, computational modeling revealed that only γ-TuNA inhibition suppressed hotspot formation. We conclude that hotspots require γ-TuNA activity, which facilitates clustered GDMT nucleation at distinct Golgi sites. © 2017 Sanders et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  17. The Turkism Idea in Kızıl Tuğ Novel

    Directory of Open Access Journals (Sweden)

    Ahmet İhsan KAYA

    2016-06-01

    Full Text Available Kızıl Tuğ is the first and most important novel by Abdullah Ziya Kozanoğlu, who was a writer of history novels. The novel, published in 1927, was among the best seller until 1980s. Kızıl Tuğ, the important history adventure novels of the Turkish Literature, was also the first history novel of the Republican Period of Turkish Literature. It acted as a source for comic books newly entering the Turkish Literature and as a Pioneer for Yeşilçam movies narrating heroic figures in The Turkish history the novel influenced the following history novelists in this respect. In addition to its popularity among the youth and the children, this work of literature still has effects on contemporary intellectuals and politicians. Kızıl Tuğ narrates the Middle Asian Turkish history in the first quarter of 1200s. The author focuses on the importance of the Turkish Union while narrating the war between Genghis Khan and China. Kızıl Tuğ was written under the idea of nation-state, and it was fictionalized to express the sovereignty of The Turkish nation and the origins of The Turkish people. Kızıl Tuğ, which has an important place in Turkish culture and literature, is of great importance in Turkish Literature since it reflects A. Ziya Kozanoğlu’s perspective on Turkish nation and his concept of Turkism

  18. Regulation of KV channel voltage-dependent activation by transmembrane β subunits

    Directory of Open Access Journals (Sweden)

    Xiaohui eSun

    2012-04-01

    Full Text Available Voltage-activated K+ (KV channels are important for shaping action potentials and maintaining resting membrane potential in excitable cells. KV channels contain a central pore-gate domain (PGD surrounded by four voltage-sensing domains (VSD. The VSDs will change conformation in response to alterations of the membrane potential thereby inducing the opening of the PGD. Many KV channels are heteromeric protein complexes containing auxiliary β subunits. These β subunits modulate channel expression and activity to increase functional diversity and render tissue specific phenotypes. This review focuses on the KV β subunits that contain transmembrane (TM segments including the KCNE family and the β subunits of large conductance, Ca2+- and voltage-activated K+ (BK channels. These TM β subunits affect the voltage-dependent activation of KV α subunits. Experimental and computational studies have described the structural location of these β subunits in the channel complexes and the biophysical effects on VSD activation, PGD opening and VSD-PGD coupling. These results reveal some common characteristics and mechanistic insights into KV channel modulation by TM β subunits.

  19. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    alters its quaternary structure: the EF-Tu-Ts cannot be covalently attached to the other enzyme subunits with bifunctional cross-linking reagents in the presence of RNA. This conformational change is not influenced by ionic strength. The addition of Qbeta RNA to the enzyme, does not result in the release...... for one another increases with increasing ionic strength. The enzyme is capable of initiation of RNA synthesis with synthetic templates only when in the low ionic strength conformation. Elongation of initiated polynucleotide chains is not affectedby ionic strength. Addition of Qbeta RNA to the enzyme also...... of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP...

  20. Retigabine, a Kv7.2/Kv7.3-Channel Opener, Attenuates Drug-Induced Seizures in Knock-In Mice Harboring Kcnq2 Mutations.

    Science.gov (United States)

    Ihara, Yukiko; Tomonoh, Yuko; Deshimaru, Masanobu; Zhang, Bo; Uchida, Taku; Ishii, Atsushi; Hirose, Shinichi

    2016-01-01

    The hetero-tetrameric voltage-gated potassium channel Kv7.2/Kv7.3, which is encoded by KCNQ2 and KCNQ3, plays an important role in limiting network excitability in the neonatal brain. Kv7.2/Kv7.3 dysfunction resulting from KCNQ2 mutations predominantly causes self-limited or benign epilepsy in neonates, but also causes early onset epileptic encephalopathy. Retigabine (RTG), a Kv7.2/ Kv7.3-channel opener, seems to be a rational antiepileptic drug for epilepsies caused by KCNQ2 mutations. We therefore evaluated the effects of RTG on seizures in two strains of knock-in mice harboring different Kcnq2 mutations, in comparison to the effects of phenobarbital (PB), which is the first-line antiepileptic drug for seizures in neonates. The subjects were heterozygous knock-in mice (Kcnq2Y284C/+ and Kcnq2A306T/+) bearing the Y284C or A306T Kcnq2 mutation, respectively, and their wild-type (WT) littermates, at 63-100 days of age. Seizures induced by intraperitoneal injection of kainic acid (KA, 12mg/kg) were recorded using a video-electroencephalography (EEG) monitoring system. Effects of RTG on KA-induced seizures of both strains of knock-in mice were assessed using seizure scores from a modified Racine's scale and compared with those of PB. The number and total duration of spike bursts on EEG and behaviors monitored by video recording were also used to evaluate the effects of RTG and PB. Both Kcnq2Y284C/+ and Kcnq2A306T/+ mice showed significantly more KA-induced seizures than WT mice. RTG significantly attenuated KA-induced seizure activities in both Kcnq2Y284C/+ and Kcnq2A306T/+ mice, and more markedly than PB. This is the first reported evidence of RTG ameliorating KA-induced seizures in knock-in mice bearing mutations of Kcnq2, with more marked effects than those observed with PB. RTG or other Kv7.2-channel openers may be considered as first-line antiepileptic treatments for epilepsies resulting from KCNQ2 mutations.

  1. T Cell Subset and Stimulation Strength-Dependent Modulation of T Cell Activation by Kv1.3 Blockers.

    Directory of Open Access Journals (Sweden)

    Wai-Ping Fung-Leung

    Full Text Available Kv1.3 is a voltage-gated potassium channel expressed on T cells that plays an important role in T cell activation. Previous studies have shown that blocking Kv1.3 channels in human T cells during activation results in reduced calcium entry, cytokine production, and proliferation. The aim of the present study was to further explore the effects of Kv1.3 blockers on the response of different human T cell subsets under various stimulation conditions. Our studies show that, unlike the immune suppressor cyclosporine A, the inhibitory effect of Kv1.3 blockers was partial and stimulation strength dependent, with reduced inhibitory efficacy on T cells under strengthened anti-CD3/CD28 stimulations. T cell responses to allergens including house dust mites and ragweed were partially reduced by Kv1.3 blockers. The effect of Kv1.3 inhibition was dependent on T cell subsets, with stronger effects on CCR7- effector memory compared to CCR7+ central memory CD4 T cells. Calcium entry studies also revealed a population of CD4 T cells resistant to Kv1.3 blockade. Activation of CD4 T cells was accompanied with an increase in Kv1.3 currents but Kv1.3 transcripts were found to be reduced, suggesting a posttranscriptional mechanism in the regulation of Kv1.3 activities. In summary, Kv1.3 blockers inhibit T cell activation in a manner that is highly dependent on the T cell identity and stimulation strength, These findings suggest that Kv1.3 blockers inhibit T cells in a unique, conditional manner, further refining our understanding of the therapeutic potential of Kv1.3 blockers.

  2. Immunomodulatory effects of diclofenac in leukocytes through the targeting of Kv1.3 voltage-dependent potassium channels.

    Science.gov (United States)

    Villalonga, Núria; David, Miren; Bielańska, Joanna; González, Teresa; Parra, David; Soler, Concepció; Comes, Núria; Valenzuela, Carmen; Felipe, Antonio

    2010-09-15

    Kv1.3 plays a crucial role in the activation and proliferation of T-lymphocytes and macrophages. While Kv1.3 is responsible for the voltage-dependent potassium current in T-cells, in macrophages this K(+) current is generated by the association of Kv1.3 and Kv1.5. Patients with autoimmune diseases show a high number of effector memory T cells that are characterized by a high expression of Kv1.3 and Kv1.3 antagonists ameliorate autoimmune disorders in vivo. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) used in patients who suffer from painful autoimmune diseases such as rheumatoid arthritis. In this study, we show that diclofenac impairs immune response via a mechanism that involves Kv1.3. While diclofenac inhibited Kv1.3 expression in activated macrophages and T-lymphocytes, Kv1.5 remained unaffected. Diclofenac also decreased iNOS levels in Raw 264.7 cells, impairing their activation in response to lipopolysaccharide (LPS). LPS-induced macrophage migration and IL-2 production in stimulated Jurkat T-cells were also blocked by pharmacological doses of diclofenac. These effects were mimicked by Margatoxin, a specific Kv1.3 inhibitor, and Charybdotoxin, which blocks both Kv1.3 and Ca(2+)-activated K(+) channels (K(Ca)3.1). Because Kv1.3 is a very good target for autoimmune therapies, the effects of diclofenac on Kv1.3 are of high pharmacological relevance. Copyright 2010 Elsevier Inc. All rights reserved.

  3. Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues.

    Science.gov (United States)

    Sato, Aya; Suematsu, Takuma; Aihara, Koh-Ki; Kita, Kiyoshi; Suzuki, Tsutomu; Watanabe, Kimitsuna; Ohtsuki, Takashi; Watanabe, Yoh-Ichi

    2017-03-07

    Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegans C. elegans EF-Tu2 specifically recognizes the seryl moiety of serylated D-armless tRNAs. Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: T-armless tRNAs, D-armless tRNAs, and cloverleaf tRNAs with a short T-arm. Trichinella mt EF-Tu1 binds to all three types and EF-Tu2 binds only to D-armless Ser-tRNAs, showing an evolutionary intermediate state from canonical EF-Tu to chromadorean nematode (e.g. C. elegans ) EF-Tu species. We report here that two EF-Tu species also participate in Drosophila melanogaster mitochondria. Both D. melanogaster EF-Tu1 and EF-Tu2 bound to cloverleaf and D-armless tRNAs. D. melanogaster EF-Tu1 has the ability to recognize T-armless tRNAs that do not evidently exist in D. melanogaster mitochondria, but do exist in related arthropod species. In addition, D. melanogaster EF-Tu2 preferentially bound to aa-tRNAs carrying small amino acids, but not to aa-tRNAs carrying bulky amino acids. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems. © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

  4. High reliability low jitter 80 kV pulse generator

    International Nuclear Information System (INIS)

    Savage, Mark Edward; Stoltzfus, Brian Scott

    2009-01-01

    Switching can be considered to be the essence of pulsed power. Time accurate switch/trigger systems with low inductance are useful in many applications. This article describes a unique switch geometry coupled with a low-inductance capacitive energy store. The system provides a fast-rising high voltage pulse into a low impedance load. It can be challenging to generate high voltage (more than 50 kilovolts) into impedances less than 10 (Omega), from a low voltage control signal with a fast rise time and high temporal accuracy. The required power amplification is large, and is usually accomplished with multiple stages. The multiple stages can adversely affect the temporal accuracy and the reliability of the system. In the present application, a highly reliable and low jitter trigger generator was required for the Z pulsed-power facility [M. E. Savage, L. F. Bennett, D. E. Bliss, W. T. Clark, R. S. Coats,J. M. Elizondo, K. R. LeChien, H. C. Harjes, J. M. Lehr, J. E. Maenchen, D. H. McDaniel, M. F. Pasik, T. D. Pointon, A. C. Owen, D. B. Seidel, D. L. Smith, B. S. Stoltzfus, K.W. Struve, W.A. Stygar, L.K. Warne, and J. R. Woodworth, 2007 IEEE Pulsed Power Conference, Albuquerque, NM (IEEE, Piscataway, NJ, 2007), p. 979]. The large investment in each Z experiment demands low prefire probability and low jitter simultaneously. The system described here is based on a 100 kV DC-charged high-pressure spark gap, triggered with an ultraviolet laser. The system uses a single optical path for simultaneously triggering two parallel switches, allowing lower inductance and electrode erosion with a simple optical system. Performance of the system includes 6 ns output rise time into 5.6 (Omega), 550 ps one-sigma jitter measured from the 5 V trigger to the high voltage output, and misfire probability less than 10 -4 . The design of the system and some key measurements will be shown in the paper. We will discuss the design goals related to high reliability and low jitter. While

  5. Translation elongation factor EF-Tu modulates filament formation of actin-like MreB protein in vitro.

    Science.gov (United States)

    Defeu Soufo, Hervé Joël; Reimold, Christian; Breddermann, Hannes; Mannherz, Hans G; Graumann, Peter L

    2015-04-24

    EF-Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was active in dynamic light-scattering assays, polymerizing in milliseconds after addition of magnesium. Purified EF-Tu enhanced the amount of MreB filaments, as seen by sedimentation assays, the speed of filament formation and the length of MreB filaments in vitro. EF-Tu had the strongest impact on MreB filaments in a 1:1 ratio, and EF-Tu co-sedimented with MreB filaments, revealing a stoichiometric interaction between both proteins. This was supported by cross-linking assays where 1:1 species were well detectable. When expressed in E. coli cells, B. subtilis MreB formed filaments and induced the formation of co-localizing B. subtilis EF-Tu structures, indicating that MreB can direct the positioning of EF-Tu structures in a heterologous cell system. Fluorescence recovery after photobleaching analysis showed that MreB filaments have a higher turnover in B. subtilis cells than in E. coli cells, indicating different filament kinetics in homologous or heterologous cell systems. The data show that MreB can direct the localization of EF-Tu in vivo, which in turn positively affects the formation and dynamics of MreB filaments. Thus, EF-Tu is a modulator of the activity of a bacterial actin-like protein. Copyright © 2015. Published by Elsevier Ltd.

  6. Comparative measurement of sup(99m)Tc-thyroid-uptake (TcTU) using two different methods of data analysis

    International Nuclear Information System (INIS)

    Hotze, L.A.; Schmitz, R.

    1982-01-01

    A new program for automatic measurement of TcTU was developed and compared with a standard interactive method of data analysis. The new program fullfils the requirements for correct calculation of TcTU. The advantages are: reduced working load for the technician and a diminished frequency of erroneous results by improving automatisation of data handling and procedure of activity measurement as well as detailed informative dialogue with the computer without any need for special knowledge of data processing. (orig.) [de

  7. Marys Lake 69/115-kV transmission line upgrade and substation expansion projects

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    Western Area Power Administration (Western) and the Platte River Power Authority (Platte River) propose to upgrade portions of the existing electric transmission and substation system that serves the Town of Estes Park, Colorado. The existing transmission lines between the Estes Power Plant Switchyard and the Marys Lake Substation include a 115,000 volt (115-kV) line and 69,000 volt (69-kV) line. Approximately one mile is a double-circuit 115/69-kV line on steel lattice structures, and approximately two miles consists of separate single-circuit 115-kV and a 69-kV lines, constructed on wood H-Frame structures. Both lines were constructed in 1951 by the US Bureau of Reclamation. The existing transmission lines are on rights-of-way (ROW) that vary from 75 feet to 120 feet and are owned by Western. There are 48 landowners adjacent to the existing ROW. All of the houses were built adjacent to the existing ROW after the transmission lines were constructed. Upgrading the existing 69-kV transmission line between the Marys Lake Substation and the Estes Power Plant Switchyard to 115-kV and expanding the Marys Lake Substation was identified as the most effective way in which to improve electric service to Estes Park. The primary purpose and need of the proposed project is to improve the reliability of electric service to the Town of Estes Park. Lack of reliability has been a historical concern, and reliability will always be less than desired until physical improvements are made to the electrical facilities serving Estes Park.

  8. Quality control of a kV cone beam computed tomography imaging system

    International Nuclear Information System (INIS)

    Marguet, M.; Bodez, V.

    2009-01-01

    Purpose: This work presents the introduction of a quality assurance program for the On-Board Imager (O.B.I., Varian) kV cone beam computed tomography (kV C.B.C.T.) system, together with the results of 1 year monthly testing. Materials and methods: Firstly the geometric precision and stability of the equipment and of the associated software were evaluated using the Marker phantom. The coincidence of the accelerator isocenter and the imager isocenter was verified as well as the accuracy of the registration of kV cone beam computed tomography (kV C.B.C.T.) with reference CT images. Then, the kV C.B.C.T. image quality was evaluated using the Catphan 504 phantom and ArtiScan software (Aquilab) for both full-fan (F.F.) and half-fan (H.F.) imaging modes. Results: The kV C.B.C.T. isocenter and image registration with correction of the table position were found to be within a tolerance of 2.0 mm. Concerning the kV C.B.C.T. image quality, image noise and uniformity, the Hounsfield units (HU) stability and linearity, geometric distortion and high contrast resolution were all found to be within the manufacturer's recommendations for both F.F. and H.F. modes. However, the low contrast resolution for the HF mode did not meet the manufacturer's specifications. Conclusion: The quality assurance tests introduced have defined the initial system characteristics and their evolution during a period of 1 year, demonstrating the stability of the O.B.I.. (authors)

  9. Marys Lake 69/115-kV transmission line upgrade and substation expansion projects

    International Nuclear Information System (INIS)

    1996-05-01

    Western Area Power Administration (Western) and the Platte River Power Authority (Platte River) propose to upgrade portions of the existing electric transmission and substation system that serves the Town of Estes Park, Colorado. The existing transmission lines between the Estes Power Plant Switchyard and the Marys Lake Substation include a 115,000 volt (115-kV) line and 69,000 volt (69-kV) line. Approximately one mile is a double-circuit 115/69-kV line on steel lattice structures, and approximately two miles consists of separate single-circuit 115-kV and a 69-kV lines, constructed on wood H-Frame structures. Both lines were constructed in 1951 by the US Bureau of Reclamation. The existing transmission lines are on rights-of-way (ROW) that vary from 75 feet to 120 feet and are owned by Western. There are 48 landowners adjacent to the existing ROW. All of the houses were built adjacent to the existing ROW after the transmission lines were constructed. Upgrading the existing 69-kV transmission line between the Marys Lake Substation and the Estes Power Plant Switchyard to 115-kV and expanding the Marys Lake Substation was identified as the most effective way in which to improve electric service to Estes Park. The primary purpose and need of the proposed project is to improve the reliability of electric service to the Town of Estes Park. Lack of reliability has been a historical concern, and reliability will always be less than desired until physical improvements are made to the electrical facilities serving Estes Park

  10. The DIAN-TU Next Generation Alzheimer’s prevention trial: adaptive design and disease progression model

    Science.gov (United States)

    Bateman, Randall J.; Benzinger, Tammie L.; Berry, Scott; Clifford, David B.; Duggan, Cynthia; Fagan, Anne M.; Fanning, Kathleen; Farlow, Martin R.; Hassenstab, Jason; McDade, Eric M.; Mills, Susan; Paumier, Katrina; Quintana, Melanie; Salloway, Stephen P.; Santacruz, Anna; Schneider, Lon S.; Wang, Guoqiao; Xiong, Chengjie

    2016-01-01

    INTRODUCTION The Dominantly Inherited Alzheimer Network Trials Unit (DIAN-TU) trial is an adaptive platform trial testing multiple drugs to slow or prevent the progression of Alzheimer’s disease in autosomal dominant Alzheimer’s disease (ADAD) families. With completion of enrollment of the first two drug arms, the DIAN-TU now plans to add new drugs to the platform, designated as the Next Generation Prevention Trial (NexGen). METHODS In collaboration with ADAD families, philanthropic organizations, academic leaders, the DIAN-TU Pharma Consortium, the NIH, and regulatory colleagues, the DIAN-TU developed innovative clinical study designs for the DIAN-TU NexGen trial. RESULTS Our expanded trials toolbox consists of a Disease Progression Model for ADAD, primary endpoint DIAN-TU cognitive performance composite, biomarker development, self-administered cognitive assessments, adaptive dose adjustments, and blinded data collection through the last participant completion. CONCLUSION These steps represent elements to improve efficacy of the adaptive platform trial and a continued effort to optimize prevention and treatment trials in ADAD. PMID:27583651

  11. Trafficking of Kv2.1 Channels to the Axon Initial Segment by a Novel Nonconventional Secretory Pathway

    DEFF Research Database (Denmark)

    Jensen, Camilla Stampe; Watanabe, Shoji; Stas, Jeroen Ingrid

    2017-01-01

    the localization of Kv2.1 in these two different membrane compartments in cultured rat hippocampal neurons of mixed sex. Our data uncover a unique ability of Kv2.1 channels to use two molecularly distinct trafficking pathways to accomplish this. Somatodendritic Kv2.1 channels are targeted by the conventional...... secretory pathway, whereas axonal Kv2.1 channels are targeted by a nonconventional trafficking pathway independent of the Golgi apparatus. We further identified a new AIS trafficking motif in the C-terminus of Kv2.1, and show that putative phosphorylation sites in this region are critical for the restricted.......SIGNIFICANCE STATEMENT Our study uncovered a novel mechanism that targets the Kv2.1 voltage-gated potassium channel to two distinct trafficking pathways and two distinct subcellular destinations: the somatodendritic plasma membrane and that of the axon initial segment. We also identified a distinct motif, including...

  12. Overvoltages transferred from a network of 220 kV to 15.65 kV level of the step-up transformer in HPP 'Bajina Bašta'

    Directory of Open Access Journals (Sweden)

    Vukelja Petar

    2011-01-01

    Full Text Available The paper presents the results of research in lightning surge waves and switching overvoltages transferred from a network of 220 kV to the 15.65 kV level of the step-up transformer in HPP 'Bajina Bašta'. Analysis of survey results lead to conclusion that transferred overvoltages can endanger 15.65 kV transformer windings and stator winding insulation. It was therefore suggested for the protection of the 15.65 kV isolation to install metal oxide surge arresters at a suitable place between the power generator bus bars and earthing.

  13. Voltage Analysis Improvement of 150 kV Transmission Subsystem Using Static Synchronous Compensator (STATCOM)

    Science.gov (United States)

    Akbar, P. A.; Hakim, D. L.; Sucita, T.

    2018-02-01

    In this research, testing improvements to the distribution voltage electricity at 150 kV transmission subsystem Bandung Selatan and New Ujungberung using Flexible AC Transmission System (FACTS) technology. One of them is by doing the control of active and reactive power through the power electronics equipment Static Synchronous Compensator (STATCOM). The subsystem is tested because it has a voltage profile are relatively less well when based on the IEEE / ANSI C.84.1 (142.5 - 157.5 kV). This study was conducted by analyzing the Newton-Raphson power flow on the simulator DigSilent Power Factory 15 to determine the profile of the voltage (V) on the system. Bus which has the lowest voltage to be a reference in the installation of STATCOM. From this research is known that the voltage on the conditions of the existing bus 28, as many as 21-23 still below standard buses (142.5 kV), after the installation is done using STATCOM, voltage on the buses improved by increasing the number of tracks that follow the standard / is in the range 142.5 kV -157.5 kV as many as 23-27 buses or 78.6% - 96%, with the optimum mounting on a bus Rancaekek STATCOM II with a capacity of 300 MVA.

  14. Clofazimine inhibits human Kv1.3 potassium channel by perturbing calcium oscillation in T lymphocytes.

    Directory of Open Access Journals (Sweden)

    Yunzhao R Ren

    Full Text Available The Kv1.3 potassium channel plays an essential role in effector memory T cells and has been implicated in several important autoimmune diseases including multiple sclerosis, psoriasis and type 1 diabetes. A number of potent small molecule inhibitors of Kv1.3 channel have been reported, some of which were found to be effective in various animal models of autoimmune diseases. We report herein the identification of clofazimine, a known anti-mycobacterial drug, as a novel inhibitor of human Kv1.3. Clofazimine was initially identified as an inhibitor of intracellular T cell receptor-mediated signaling leading to the transcriptional activation of human interleukin-2 gene in T cells from a screen of the Johns Hopkins Drug Library. A systematic mechanistic deconvolution revealed that clofazimine selectively blocked the Kv1.3 channel activity, perturbing the oscillation frequency of the calcium-release activated calcium channel, which in turn led to the inhibition of the calcineurin-NFAT signaling pathway. These effects of clofazimine provide the first line of experimental evidence in support of a causal relationship between Kv1.3 and calcium oscillation in human T cells. Furthermore, clofazimine was found to be effective in blocking human T cell-mediated skin graft rejection in an animal model in vivo. Together, these results suggest that clofazimine is a promising immunomodulatory drug candidate for treating a variety of autoimmune disorders.

  15. Identification of a functional interaction between Kv4.3 channels and c-Src tyrosine kinase.

    Science.gov (United States)

    Gomes, Pedro; Saito, Tomoaki; Del Corsso, Cris; Alioua, Abderrahmane; Eghbali, Mansoureh; Toro, Ligia; Stefani, Enrico

    2008-10-01

    Voltage-gated K(+) (Kv) channels are key determinants of cardiac and neuronal excitability. A substantial body of evidence has accumulated in support of a role for Src family tyrosine kinases in the regulation of Kv channels. In this study, we examined the possibility that c-Src tyrosine kinase participates in the modulation of the transient voltage-dependent K(+) channel Kv4.3. Supporting a mechanistic link between Kv4.3 and c-Src, confocal microscopy analysis of HEK293 cells stably transfected with Kv4.3 showed high degree of co-localization of the two proteins at the plasma membrane. Our results further demonstrate an association between Kv4.3 and c-Src by co-immunoprecipitation and GST pull-down assays, this interaction being mediated by the SH2 and SH3 domains of c-Src. Furthermore, we show that Kv4.3 is tyrosine phosphorylated under basal conditions. The functional relevance of the observed interaction between Kv4.3 and c-Src was established in patch-clamp experiments, where application of the Src inhibitor PP2 caused a decrease in Kv4.3 peak current amplitude, but not the inactive structural analogue PP3. Conversely, intracellular application of recombinant c-Src kinase or the protein tyrosine phosphatase inhibitor bpV(phen) increased Kv4.3 peak current amplitude. In conclusion, our findings provide evidence that c-Src-induced Kv4.3 channel activation involves their association in a macromolecular complex and suggest a role for c-Src-Kv4.3 pathway in regulating cardiac and neuronal excitability.

  16. Design and operation of a setup with a camera and adjustable mirror to inspect the sense-wire planes of the Time Projection Chamber inside the MicroBooNE cryostat

    International Nuclear Information System (INIS)

    Carls, B.; James, C.C.; Kubinski, R.M.; Pordes, S.; Schukraft, A.; Horton-Smith, G.; Strauss, T.

    2015-01-01

    Detectors in particle physics, particularly when including cryogenic components, are often enclosed in vessels that do not provide any physical or visual access to the detectors themselves after installation. However, it can be desirable for experiments to visually investigate the inside of the vessel. The MicroBooNE cryostat hosts a TPC with sense-wire planes, which had to be inspected for damage such as breakage or sagging. This inspection was performed after the transportation of the vessel with the enclosed detector to its final location, but before filling with liquid argon. This paper describes an approach to view the inside of the MicroBooNE cryostat with a setup of a camera and a mirror through one of its cryogenic service nozzles. The paper describes the camera and mirror chosen for the operation, the illumination, and the mechanical structure of the setup. It explains how the system was operated and demonstrates its performance

  17. Development of Highly Selective Kv1.3-Blocking Peptides Based on the Sea Anemone Peptide ShK

    Directory of Open Access Journals (Sweden)

    Michael W. Pennington

    2015-01-01

    Full Text Available ShK, from the sea anemone Stichodactyla helianthus, is a 35-residue disulfide-rich peptide that blocks the voltage-gated potassium channel Kv1.3 at ca. 10 pM and the related channel Kv1.1 at ca. 16 pM. We developed an analog of this peptide, ShK-186, which is currently in Phase 1b-2a clinical trials for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. While ShK-186 displays a >100-fold improvement in selectivity for Kv1.3 over Kv1.1 compared with ShK, there is considerable interest in developing peptides with an even greater selectivity ratio. In this report, we describe several variants of ShK that incorporate p-phophono-phenylalanine at the N-terminus coupled with internal substitutions at Gln16 and Met21. In addition, we also explored the combinatorial effects of these internal substitutions with an alanine extension at the C-terminus. Their selectivity was determined by patch-clamp electrophysiology on Kv1.3 and Kv1.1 channels stably expressed in mouse fibroblasts. The peptides with an alanine extension blocked Kv1.3 at low pM concentrations and exhibited up to 2250-fold selectivity for Kv1.3 over Kv1.1. Analogs that incorporates p-phosphono-phenylalanine at the N-terminus blocked Kv1.3 with IC50s in the low pM range and did not affect Kv1.1 at concentrations up to 100 nM, displaying a selectivity enhancement of >10,000-fold for Kv1.3 over Kv1.1. Other potentially important Kv channels such as Kv1.4 and Kv1.6 were only partially blocked at 100 nM concentrations of each of the ShK analogs.

  18. Blockade of the voltage-gated potassium channel Kv1.3 inhibits immune responses in vivo.

    Science.gov (United States)

    Koo, G C; Blake, J T; Talento, A; Nguyen, M; Lin, S; Sirotina, A; Shah, K; Mulvany, K; Hora, D; Cunningham, P; Wunderler, D L; McManus, O B; Slaughter, R; Bugianesi, R; Felix, J; Garcia, M; Williamson, J; Kaczorowski, G; Sigal, N H; Springer, M S; Feeney, W

    1997-06-01

    The voltage activated K+ channel (Kv1.3) has recently been identified as the molecule that sets the resting membrane potential of peripheral human T lymphoid cells. In vitro studies indicate that blockage of Kv1.3 inhibits T cell activation, suggesting that Kv1.3 may be a target for immunosuppression. However, despite the in vitro evidence, there has been no in vivo demonstration that blockade of Kv1.3 will attenuate an immune response. The difficulty is due to species differences, as the channel does not set the membrane potential in rodent peripheral T cells. In this study, we show that the channel is present on peripheral T cells of miniswine. Using the peptidyl Kv1.3 inhibitor, margatoxin, we demonstrate that Kv1.3 also regulates the resting membrane potential, and that blockade of Kv1.3 inhibits, in vivo, both a delayed-type hypersensitivity reaction and an Ab response to an allogeneic challenge. In addition, prolonged Kv1.3 blockade causes reduced thymic cellularity and inhibits the thymic development of T cell subsets. These results provide in vivo evidence that Kv1.3 is a novel target for immunomodulation.

  19. The Seismic Analysis of 800kV Gas Insulated Switchgear (GIS) for the Dangjin Thermal Plant

    Energy Technology Data Exchange (ETDEWEB)

    Shin, I.H.; Song, W.P.; Kweon, K.Y. [Hyosung Corporation (Korea)

    1999-05-01

    800kV GIS (Gas Insulated Switchgear) which was first developed in korea at Dec. 1998 and is going to be installed in the dangjin thermal plant. We checked the stability of 800kV GIS under seismic load. pro-ENGINEER and PATRAN were used for modeling exactly 800kV GIS geometry. The 800kV GIS was modeled as shell elements for the enclosures and beam elements for the conductors and the support insulators. (author). 2 refs., 9 figs., 2 tabs.

  20. Töötu laevamehhaanik õppis seitsme kuuga kokaks / Agne Narusk

    Index Scriptorium Estoniae

    Narusk, Agne, 1971-

    2004-01-01

    Töötu Jevgeni Solovjov õppis OÜ-s Isabella kokaks, ettevõte on üks 54 koolitajast, kellelt Tallinna tööhõiveamet teenust ostab. 2004. aastaks oli Tallinna tööhõiveametil tööotsijate koolitamiseks 7 mln krooni, millest jätkus pooleks aastaks. Lisa: Tööandjad saavad küsitluslehed

  1. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification

    Czech Academy of Sciences Publication Activity Database

    Jonák, Jiří

    2007-01-01

    Roč. 849, 1-2 (2007), s. 141-153 ISSN 1570-0232 R&D Projects: GA AV ČR IAA5052206; GA AV ČR KJB500520503; GA MŠk 2B06065 Institutional research plan: CEZ:AV0Z50520514 Keywords : bacterial elongation factors EF-Tu, , G-domain * recombinant EF-Tus * preparation Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.935, year: 2007

  2. Kinetic study of the substitution of [Tc(tu)6]3+ by polyaminocarboxylic acids

    International Nuclear Information System (INIS)

    Torres, J.; Gonalez, R.; Kremer, C.; Kremer, E.; Leon, A.

    1997-01-01

    Substitution route has been traditionally proposed for the synthesis of new Tc complexes. In particular, Tc(III) coordination compounds can be successfully prepared by substitution on [Tc(tu) 6 ] 3+ (tu = thiourea). In this substitution reaction, TcO 2 is a side product that should be minimized. The success of these synthetic procedures is based on controlling the reaction conditions in order that pure substitution could be faster enough compared to decomposition. In this work, the substitution of [Tc(tu) 6 ] 3+ by polycarboxylic acids (ethylenediaminetetraacetate (edta), diethylenetriaminepentaacetate (dtpa), N-tris(2-amino-ethyl)amin N', N', N '' , N '' , N ''' , N ''' -hexaacetate (ttaha) and 3-bromo-2,4,6-trimethylacetanilideiminodiacetate (mebrofenin)) is kinetically studied in order to obtain the best conditions for preparing Tc(III) complexes. As a general conclusion, substitution constants are strongly dependent on the pH. k obs values fall in the range 0.1-13 x 10 -2 M -1 s -1 , depending on the pH value and the temperature. No strong differences are found with different incoming ligands. For the four ligands (L), a straight line is obtained when plotting log k obs /([L m- ]) vs. pH. This is consistent with a rate constant k obs = {k 0 + Σk n [H + ] n /K n }[L m- ], where n represents the charge of the incoming ligand and m is the maximum value of n. (orig.)

  3. Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat.

    Science.gov (United States)

    Schoonbeek, Henk-Jan; Wang, Hsi-Hua; Stefanato, Francesca L; Craze, Melanie; Bowden, Sarah; Wallington, Emma; Zipfel, Cyril; Ridout, Christopher J

    2015-04-01

    Perception of pathogen (or microbe)-associated molecular patterns (PAMPs/MAMPs) by pattern recognition receptors (PRRs) is a key component of plant innate immunity. The Arabidopsis PRR EF-Tu receptor (EFR) recognizes the bacterial PAMP elongation factor Tu (EF-Tu) and its derived peptide elf18. Previous work revealed that transgenic expression of AtEFR in Solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. In this study, we developed a set of bioassays to study the activation of PAMP-triggered immunity (PTI) in wheat. We generated transgenic wheat (Triticum aestivum) plants expressing AtEFR driven by the constitutive rice actin promoter and tested their response to elf18. We show that transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition. When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication. These results demonstrate that AtEFR can be transferred successfully from dicot to monocot species, further revealing that immune signalling pathways are conserved across these distant phyla. As novel PRRs are identified, their transfer between plant families represents a useful strategy for enhancing resistance to pathogens in crops. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  4. 600 kV modulator design for the SLAC Next Linear Collider Test Accelerator

    International Nuclear Information System (INIS)

    Harris, K.; de Lamare, J.; Nesterov, V.; Cassel, R.

    1992-07-01

    Preliminary design for the SLAC Next Linear Collider Test Accelerator (NLCTA) requires a pulse power source to produce a 600 kV, 600 A, 1.4 μs, 0.1% flat top pulse with rise and fall times of approximately 100 ns to power an X-Band klystron with a microperveance of 1.25 at ∼ 100 MW peak RF power. The design goals for the modulator, including those previously listed, are peak modulator pulse power of 340 MW operating at 120 Hz. A three-stage darlington pulse-forming network, which produces a >100 kV, 1.4 μs pulse, is coupled to the klystron load through a 6:1 pulse transformer. Careful consideration of the transformer leakage inductance, klystron capacitance, system layout, and component choice is necessary to produce the very fast rise and fall times at 600 kV operating continuously at 120 Hz

  5. 735 kV air-blast circuit breakers for Hydro Quebec

    Energy Technology Data Exchange (ETDEWEB)

    Lindstrom, I

    1966-01-01

    In 1962 the Quebec Hydro-Electric Commission started the development of aproject for utilizing water power in Manicouagan and Outardes, two rivers some 350 miles northeast of the city of Montreal on the left bank of the St. Lawrence estuary. The project involved the transmission of power at 735 kV ac from Manicouagan and Outardes to the heavy and growing load centers of Quebec and Montreal. The total amount of energy to be transmitted in the final stage is about 6,000 MW. The project involved the need of considerable quantities of apparatus for 735 kV, such as transformers, reactors, lightning arrestors, circuit-breakers, and disconnectors. Since the supply of the major part of the 735 kV circuit-breakers, altogether 14 three-pole units, was awarded to ASEA in Sweden, a short presentation of these airblast circuit-breakers is presented.

  6. Field Demonstration of a 24-kV Superconducting Cable at Detroit Edison

    International Nuclear Information System (INIS)

    Kelley, Nathan; Corsaro, Pietro

    2004-01-01

    Customer acceptance of high temperature superconducting (HTS) cable technology requires a substantial field demonstration illustrating both the system's technical capabilities and its suitability for installation and operation within the utility environment. In this project, the world's first underground installation of an HTS cable using existing ductwork, a 120 meter demonstration cable circuit was designed and installed between the 24 kV bus distribution bus and a 120 kV-24 kV transformer at Detroit Edison's Frisbie substation. The system incorporated cables, accessories, a refrigeration system, and control instrumentation. Although the system was never put in operation because of problems with leaks in the cryostat, the project significantly advanced the state-of-the-art in the design and implementation of Warm Dielectric cable systems in substation applications. Lessons learned in this project are already being incorporated in several ongoing demonstration projects

  7. Rearrangement of potassium ions and Kv1.1/Kv1.2 potassium channels in regenerating axons following end-to-end neurorrhaphy: ionic images from TOF-SIMS.

    Science.gov (United States)

    Liu, Chiung-Hui; Chang, Hung-Ming; Wu, Tsung-Huan; Chen, Li-You; Yang, Yin-Shuo; Tseng, To-Jung; Liao, Wen-Chieh

    2017-10-01

    The voltage-gated potassium channels Kv1.1 and Kv1.2 that cluster at juxtaparanodal (JXP) regions are essential in the regulation of nerve excitability and play a critical role in axonal conduction. When demyelination occurs, Kv1.1/Kv1.2 activity increases, suppressing the membrane potential nearly to the equilibrium potential of K + , which results in an axonal conduction blockade. The recovery of K + -dependent communication signals and proper clustering of Kv1.1/Kv1.2 channels at JXP regions may directly reflect nerve regeneration following peripheral nerve injury. However, little is known about potassium channel expression and its relationship with the dynamic potassium ion distribution at the node of Ranvier during the regenerative process of peripheral nerve injury (PNI). In the present study, end-to-end neurorrhaphy (EEN) was performed using an in vivo model of PNI. The distribution of K + at regenerating axons following EEN was detected by time-of-flight secondary-ion mass spectrometry. The specific localization and expression of Kv1.1/Kv1.2 channels were examined by confocal microscopy and western blotting. Our data showed that the re-establishment of K + distribution and intensity was correlated with the functional recovery of compound muscle action potential morphology in EEN rats. Furthermore, the re-clustering of Kv1.1/1.2 channels 1 and 3 months after EEN at the nodal region of the regenerating nerve corresponded to changes in the K + distribution. This study provided direct evidence of K + distribution in regenerating axons for the first time. We proposed that the Kv1.1/Kv1.2 channels re-clustered at the JXP regions of regenerating axons are essential for modulating the proper patterns of K + distribution in axons for maintaining membrane potential stability after EEN.

  8. In vitro measurement of CT density and estimation of stenosis related to coronary soft plaque at 100 kV and 120 kV on ECG-triggered scan

    Energy Technology Data Exchange (ETDEWEB)

    Horiguchi, Jun, E-mail: horiguch@hiroshima-u.ac.jp [Department of Clinical Radiology, Hiroshima University Hospital, 1-2-3, Kasumi-cho, Minami-ku, Hiroshima 734-8551 (Japan); Fujioka, Chikako, E-mail: fujioka@hiroshima-u.ac.jp [Department of Clinical Radiology, Hiroshima University Hospital, 1-2-3, Kasumi-cho, Minami-ku, Hiroshima 734-8551 (Japan); Kiguchi, Masao, E-mail: kiguchi@hiroshima-u.ac.jp [Department of Clinical Radiology, Hiroshima University Hospital, 1-2-3, Kasumi-cho, Minami-ku, Hiroshima 734-8551 (Japan); Yamamoto, Hideya, E-mail: hideyayama@hiroshima-u.ac.jp [Department of Cardiovascular Medicine, Hiroshima University Graduate School of Biomedical Sciences and Hiroshima University Hospital, 1-2-3, Kasumi-cho, Minami-ku, Hiroshima 734-8551 (Japan); Shen, Yun, E-mail: Yuna.Shen@ge.com [CT Lab of Great China, GE Healthcare, L12 and L15, Office Tower, Langham Place, 8 Argyle Street, Mongkok Kowloon (Hong Kong); Kihara, Yasuki, E-mail: ykihara@hiroshima-u.ac.jp [Department of Cardiovascular Medicine, Hiroshima University Graduate School of Biomedical Sciences and Hiroshima University Hospital, 1-2-3, Kasumi-cho, Minami-ku, Hiroshima 734-8551 (Japan)

    2011-02-15

    Purpose: The purpose of the study was to compare 100 kV and 120 kV prospective electrocardiograph (ECG)-triggered axial coronary 64-detector CT angiography (64-MDCTA) in soft plaque diagnosis. Materials and methods: Coronary artery models (n = 5) with artificial soft plaques (-32 HU to 53 HU at 120 kV) with three stenosis levels (25%, 50% and 75%) on a cardiac phantom (mimicking slim patient's environment) were scanned in heart rates of 55, 60 and 65 beats per minute (bpm). Four kinds of intracoronary enhancement (205 HU, 241 HU, 280 HU and 314 HU) were simulated. The soft plaque density and the measurement error of stenosis (in percentage), evaluated by two independent observers, were compared between 100 kV and 120 kV. The radiation dose was estimated. Results: Interobserver correlation of the measurement was excellent (density; r = 0.95 and stenosis measure; r = 0.97). Neither the density of soft plaque nor the measurement error of stenosis was different between 100 kV and 120 kV (p = 0.22 and 0.08). The estimated radiation doses were 2.0 mSv and 3.3 mSv (in 14 cm coverage) on 100 kV and 120 kV prospective ECG-triggered axial scans, respectively. Conclusion: The 100 kV prospective ECG-triggered coronary MDCTA has comparable performance to 120 kV coronary CTA in terms of soft plaque densitometry and measurement of stenosis, with a reduced effective dose of 2 mSv.

  9. Improvement the Capacity of Cockcroft-Walton High Voltage Source from 300 kV/20 mA to 500 kV/20 mA for Accelerating Voltage of Electron Beam Machine

    International Nuclear Information System (INIS)

    Suprapto; Djasiman

    2002-01-01

    The improvement capacity of Cockcroft-Walton high voltage source from 300 kV/20 mA to 500 kV/mA has been carrying out. To improve the capacity of high voltage source was done by means of increasing the stage number of voltage multiplier from 11 to 18 and its output voltage measuring resistance. Each stage of voltage multiplier consists of 2 capacitors and 2 circuits of high voltage diode. This voltage multiplier is constructed using main components of high voltage capacitor and high voltage diode each of 0.22 μF/50 kV and UF 5408 respectively. To avoid stray discharge and corona it was provided with high voltage electrode and corona ring. The test result indicated that the output voltage obtained from 16 stages was 350 kV according to operating condition of 25 MΩ resistive load and first stage voltage of 28.5 kV with oscillator frequency of 24 Hz. That condition requires anode voltage and current of 5.5 kV and 2.5 A respectively. The no load test for 16 stages indicates 400 kV of output voltage and 28.5 kV first stage voltage. Efficiency of high voltage source was 48 % at 6.75 kW of output power. The expected test of 500 kV with 18 stages of voltage multiplier can not be carried out because of some restrictive of loading system. From the test result can be predicted that the output voltage of 500 kV with 18 stages of voltage multiplier requires 31.2 kV of first stage voltage. Then the expected high voltage source of Cockcroft-Walton is capable as accelerating voltage source for Electron Beam Machine with energy of 500 kV. (author)

  10. Evaluation of variation of voltage (kV) absorbed dose in chest CT scans

    International Nuclear Information System (INIS)

    Mendonca, Bruna G.A.; Mourao, Arnaldo P.

    2013-01-01

    Computed tomography (CT) is one of the most important diagnostic techniques images today. The increasing utilization of CT implies a significant increase of population exposure to ionizing radiation. Optimization of practice aims to reduce doses to patients because the image quality is directly related to the diagnosis. You can decrease the amount of dose to the patient, and maintain the quality of the image. There are several parameters that can be manipulated in a CT scan and these parameters can be used to reduce the energy deposited in the patient. Based on this, we analyzed the variation of dose deposited in the lungs, breasts and thyroid, by varying the supply voltage of the tube. Scans of the thorax were performed following the protocol of routine chest with constant and variable current for the same applied voltage. Moreover, a female phantom was used and thermoluminescent dosimeters (TLD-100), model bat, were used to record the specific organ doses. Scans were performed on a GE CT scanner, model 64 Discovery channels. Higher doses were recorded for the voltage of 120 kV with 200 mAs in the lungs (22.46 mGy) and thyroid (32.22 mGy). For scans with automatic mAs, variable between 100 and 440, this same tension contributed to the higher doses. The best examination in terms of the dose that was used with automatic 80 kV mAs, whose lungs and thyroid received lower dose. For the best breast exam was 100 kV. Since the increase in the 80 kV to 100 kV no impact so much the dose deposited in the lungs, it can be concluded that lowering the applied voltage to 100 kV resulted in a reduction in the dose absorbed by the patient. These results can contribute to optimizing scans of the chest computed tomography

  11. Switching Overvoltages in 60 kV reactor compensated cable grid due to resonance after disconnection

    DEFF Research Database (Denmark)

    Bak, Claus Leth; Baldursson, Haukur; Oumarou, Abdoul M.

    2008-01-01

    power could be directly connected to long cables. Switching both cable and reactor together will cause resonance to occur between the cable capacitance and the inductance of the cable during last end disconnection. Similar type of resonance condition is known to have caused switching overvoltages...... on the 400kV grid in Denmark. Therefore it is considered necessary to analyze further whether connecting a reactor directly to 60kV cable can cause switching overvoltages. A model in PSCAD was used to analyze which parameters can cause overvoltage. The switching resonance overvoltage was found to be caused...

  12. Performance of a 100-kV, 78-kJ electric-gun system

    International Nuclear Information System (INIS)

    Chau, H.; Dittbenner, G.; Mikkelsen, K.; Weingart, R.; Froeschner, K.; Lee, R.

    1981-01-01

    A new electric gun system was constructed for use in high-pressure EOS studies. The system is powered by a 100 kV, 15.6 μF capacitor bank. At 100 kV charging voltage the system inductance is 23 nH. This system has driven 0.3 mm-thick Kapton projectiles to > 20 km/s and 0.3 mm Kapton/30 μm Ta projectiles to approx. 10 km/s. Projectile velocity is modeled phenomenlogically by an electrical Gurney model

  13. High power cable with internal water cooling 400 kV

    Energy Technology Data Exchange (ETDEWEB)

    Rasquin, W; Harjes, B

    1982-08-01

    The project was planned for a duration of 4 years. Afterwards it has been extended over 6 years and finally stopped after 3 1/2 years. Therefore, of course results of field tests with an internally cooled 400 kV cable are not available. Nevertheless, this conductor cooled high power cable has been developed to such an extend, that this manufactured cable could withstand type tests according to IEC/VDE recommendations. Even by missing field tests it is obvious that a high power cable for 400 kV is available.

  14. Short-Circuit Degradation of 10-kV 10-A SiC MOSFET

    DEFF Research Database (Denmark)

    Eni, Emanuel-Petre; Beczkowski, Szymon; Munk-Nielsen, Stig

    2017-01-01

    The short-circuit behavior of power devices is highly relevant for converter design and fault protection. In this work, the degradation during short-circuit of a 10 kV 10 A 4H-SiC MOSFET is investigated at 6 kV DC-link voltage. The study aims to present the behavior of the device during short-circuit...... transients as it sustains increasing short-circuit pulses during its life-time. As the short-circuit pulse length increases, degradation of the device can be observed in periodically performed characterizations. The initial degradation seems to be associated with the channel region, and continuous stressing...

  15. Research on Line Patrol Strategy of 110kV Transmission Line after Lightning Strike

    Directory of Open Access Journals (Sweden)

    Li Mingjun

    2016-01-01

    Full Text Available Lightning faults occupy in the majority of instantaneous fault and reclosing can usually be successful, so power supply can be restored without immediate patrol in many cases. Firstly, this paper introduces the lightning fault positioning and identifying method. Then test electrical performance of insulators after lightning strike from 110kV lines. Data shows that lightning strike has little effect on the electric performance of insulator. Finally, illustrating disposal process of the 110 kV transmission line after lightning fault, certifying that the power supply reliability be ensured without line patrol.

  16. Trafficking and intracellular regulation of Kv7.1 potassium channels in the heart

    DEFF Research Database (Denmark)

    Nielsen, Nathalie Hélix

    identified. About 100 of these mutations are located in the N- or the C-terminal parts of the channel. The aim of the present work was to gain a better understanding of the Kv7.1 channel protein function. In the first study we identified a Kv7.1 missense mutation in a German family with Long QT Syndrome......The electrical activity of the heart, measured by application of surface body electrodes and recorded as an electrocardiogram, is the result of a finely tuned balance of ion movement (K+, Na+, Ca2+). The ionic currents collectively constitute the cardiac action potential created in the cell...

  17. AMP-activated protein kinase downregulates Kv7.1 cell surface expression

    DEFF Research Database (Denmark)

    Andersen, Martin N; Krzystanek, Katarzyna; Jespersen, Thomas

    2012-01-01

    in response to polarization of the epithelial Madin-Darby canine kidney (MDCK) cell line and that this was mediated by activation of protein kinase C (PKC). In this study, the pathway downstream of PKC, which leads to internalization of Kv7.1 upon cell polarization, is elucidated. We show by confocal...... microscopy that Kv7.1 is endocytosed upon initiation of the polarization process and sent for degradation by the lysosomal pathway. The internalization could be mimicked by pharmacological activation of the AMP-activated protein kinase (AMPK) using three different AMPK activators. We demonstrate...

  18. A truncated Kv1.1 protein in the brain of the megencephaly mouse: expression and interaction

    Directory of Open Access Journals (Sweden)

    Århem Peter

    2005-11-01

    Full Text Available Abstract Background The megencephaly mouse, mceph/mceph, is epileptic and displays a dramatically increased brain volume and neuronal count. The responsible mutation was recently revealed to be an eleven base pair deletion, leading to a frame shift, in the gene encoding the potassium channel Kv1.1. The predicted MCEPH protein is truncated at amino acid 230 out of 495. Truncated proteins are usually not expressed since nonsense mRNAs are most often degraded. However, high Kv1.1 mRNA levels in mceph/mceph brain indicated that it escaped this control mechanism. Therefore, we hypothesized that the truncated Kv1.1 would be expressed and dysregulate other Kv1 subunits in the mceph/mceph mice. Results We found that the MCEPH protein is expressed in the brain of mceph/mceph mice. MCEPH was found to lack mature (Golgi glycosylation, but to be core glycosylated and trapped in the endoplasmic reticulum (ER. Interactions between MCEPH and other Kv1 subunits were studied in cell culture, Xenopus oocytes and the brain. MCEPH can form tetramers with Kv1.1 in cell culture and has a dominant negative effect on Kv1.2 and Kv1.3 currents in oocytes. However, it does not retain Kv1.2 in the ER of neurons. Conclusion The megencephaly mice express a truncated Kv1.1 in the brain, and constitute a unique tool to study Kv1.1 trafficking relevant for understanding epilepsy, ataxia and pathologic brain overgrowth.

  19. Chronic electroconvulsive stimulation but not chronic restraint stress modulates mRNA expression of voltage-dependent potassium channels Kv7.2 and Kv11.1 in the rat piriform cortex

    DEFF Research Database (Denmark)

    Hjæresen, Marie-Louise; Hageman, Ida; Wörtwein, Gitta

    2008-01-01

    The mechanisms by which stress and electroconvulsive therapy exert opposite effects on the course of major depression are not known. Potential candidates might include the voltage-dependent potassium channels. Potassium channels play an important role in maintaining the resting membrane potential...... and controlling neuronal excitability. To explore this hypothesis, we examined the effects of one or several electroconvulsive stimulations and chronic restraint stress (6 h/day for 21 days) on the expression of voltage-dependent potassium channel Kv7.2, Kv11.1, and Kv11.3 mRNA in the rat brain using in situ...... hybridization. Repeated, but not acute, electroconvulsive stimulation increased Kv7.2 and Kv11.1 mRNA levels in the piriform cortex. In contrast, restraint stress had no significant effect on mRNA expression of Kv7.2, Kv11.1, or Kv11.3 in any of the brain regions examined. Thus, it appears that the investigated...

  20. Simultaneous Binding of Multiple EF-Tu Copies to Translating Ribosomes in Live Escherichia coli.

    Science.gov (United States)

    Mustafi, Mainak; Weisshaar, James C

    2018-01-16

    In bacteria, elongation factor Tu is a translational cofactor that forms ternary complexes with aminoacyl-tRNA (aa-tRNA) and GTP. Binding of a ternary complex to one of four flexible L7/L12 units on the ribosome tethers a charged tRNA in close proximity to the ribosomal A site. Two sequential tests for a match between the aa-tRNA anticodon and the current mRNA codon then follow. Because one elongation cycle can occur in as little as 50 ms and the vast majority of aa-tRNA copies are not cognate with the current mRNA codon, this testing must occur rapidly. We present a single-molecule localization and tracking study of fluorescently labeled EF-Tu in live Escherichia coli Imaging at 2 ms/frame distinguishes 60% slowly diffusing EF-Tu copies (assigned as transiently bound to translating ribosome) from 40% rapidly diffusing copies (assigned as a mixture of free ternary complexes and free EF-Tu). Combining these percentages with copy number estimates, we infer that the four L7/L12 sites are essentially saturated with ternary complexes in vivo. The results corroborate an earlier inference that all four sites can simultaneously tether ternary complexes near the A site, creating a high local concentration that may greatly enhance the rate of testing of aa-tRNAs. Our data and a combinatorial argument both suggest that the initial recognition test for a codon-anticodon match occurs in less than 1 to 2 ms per aa-tRNA copy. The results refute a recent study (A. Plochowietz, I. Farrell, Z. Smilansky, B. S. Cooperman, and A. N. Kapanidis, Nucleic Acids Res 45:926-937, 2016, https://doi.org/10.1093/nar/gkw787) of tRNA diffusion in E. coli that inferred that aa-tRNAs arrive at the ribosomal A site as bare monomers, not as ternary complexes. IMPORTANCE Ribosomes catalyze translation of the mRNA codon sequence into the corresponding sequence of amino acids within the nascent polypeptide chain. Polypeptide elongation can be as fast as 50 ms per added amino acid. Each amino acid

  1. Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

    Institute of Scientific and Technical Information of China (English)

    Fen Lu; Huiqin Wang; Shanzhi Wang; Wendi Jiang; Changlin Shan; Bin Li; Jun Yang; Shiyong Zhang; Wenxian Sun

    2015-01-01

    The elongation factor Tu (EF-Tu) receptor (EFR) in cruciferous plants specifical y recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants (Oryza sativa L. ssp. japonica cv. Zhonghua 17) and cel cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa-tion in the AtEFR transgenic rice cel s and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatical y in transgenic rice plant and cel lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig-gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expression level. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Col ectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

  2. The acrylamide (S)-2 as a positive and negative modulator of Kv7 channels expressed in Xenopus laevis oocytes

    DEFF Research Database (Denmark)

    Blom, Sigrid Marie; Schmitt, Nicole; Jensen, Henrik Sindal

    2009-01-01

    Kv7.2-5, is now in clinical trial phase III for the treatment of partial onset seizures. One of the main obstacles in developing Kv7 channel active drugs has been to identify compounds that can discriminate between the neuronal subtypes, a feature that could help diminish side effects and increase...

  3. The schizophrenia-associated Kv11.1-3.1 isoform results in reduced current accumulation during repetitive brief depolarizations.

    Directory of Open Access Journals (Sweden)

    Juliane Heide

    Full Text Available Recent genome wide association studies identified a brain and primate specific isoform of a voltage-gated potassium channel, referred to as Kv11.1-3.1, which is significantly associated with schizophrenia. The 3.1 isoform replaces the first 102 amino acids of the most abundant isoform (referred to as Kv11.1-1A with six unique amino acids. Here we show that the Kv11.1-3.1 isoform has faster rates of channel deactivation but a slowing of the rates of inactivation compared to the Kv11.1-1A isoform. The Kv11.1-3.1 isoform also has a significant depolarizing shift in the voltage-dependence of steady-state inactivation. The consequence of the altered gating kinetics is that there is lower current accumulation for Kv11.1-3.1 expressing cells during repetitive action potential firing compared to Kv11.1-1A expressing cells, which in turn will result in longer lasting trains of action potentials. Increased expression of Kv11.1-3.1 channels in the brain of schizophrenia patients might therefore contribute to disorganized neuronal firing.

  4. Physiological role of Kv1.3 channel in T lymphocyte cell investigated quantitatively by kinetic modeling.

    Directory of Open Access Journals (Sweden)

    Panpan Hou

    Full Text Available Kv1.3 channel is a delayed rectifier channel abundant in human T lymphocytes. Chronic inflammatory and autoimmune disorders lead to the over-expression of Kv1.3 in T cells. To quantitatively study the regulatory mechanism and physiological function of Kv1.3 in T cells, it is necessary to have a precise kinetic model of Kv1.3. In this study, we firstly established a kinetic model capable to precisely replicate all the kinetic features for Kv1.3 channels, and then constructed a T-cell model composed of ion channels including Ca2+-release activated calcium (CRAC channel, intermediate K+ (IK channel, TASK channel and Kv1.3 channel for quantitatively simulating the changes in membrane potentials and local Ca2+ signaling messengers during activation of T cells. Based on the experimental data from current-clamp recordings, we successfully demonstrated that Kv1.3 dominated the membrane potential of T cells to manipulate the Ca2+ influx via CRAC channel. Our results revealed that the deficient expression of Kv1.3 channel would cause the less Ca2+ signal, leading to the less efficiency in secretion. This was the first successful attempt to simulate membrane potential in non-excitable cells, which laid a solid basis for quantitatively studying the regulatory mechanism and physiological role of channels in non-excitable cells.

  5. Parameter design and performance simulation of a 10 kV voltage compensation type active superconducting fault current limiter

    International Nuclear Information System (INIS)

    Chen, L.; Tang, Y.J.; Song, M.; Shi, J.; Ren, L.

    2013-01-01

    Highlights: •For a practical 10 kV system, the 10 kV active SFCL’s basic parameters are designed. •Under different fault conditions, the 10 kV active SFCL’s performances are simulated. •The designed 10 kV active SFCL’s engineering feasibility is discussed preliminarily. -- Abstract: Since the introduction of superconducting fault current limiter (SFCL) into electrical distribution system may be a good choice with economy and practicability, the parameter design and current-limiting characteristics of a 10 kV voltage compensation type active SFCL are studied in this paper. Firstly, the SFCL’s circuit structure and operation principle are presented. Then, taking a practical 10 kV distribution system as its application object, the SFCL’s basic parameters are designed to meet the system requirements. Further, using MATLAB, the detailed current-limiting performances of the 10 kV active SFCL are simulated under different fault conditions. The simulation results show that the active SFCL can deal well with the faults, and the parameter design’s suitability can be testified. At the end, in view of the engineering feasibility of the 10 kV active SFCL, some preliminary discussions are carried out

  6. Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells

    International Nuclear Information System (INIS)

    Jang, Soo Hwa; Choi, Changsun; Hong, Seong-Geun; Yarishkin, Oleg V.; Bae, Young Min; Kim, Jae Gon; O'Grady, Scott M.; Yoon, Kyong-Ah; Kang, Kyung-Sun; Ryu, Pan Dong; Lee, So Yeong

    2009-01-01

    Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelial cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation.

  7. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons

    Directory of Open Access Journals (Sweden)

    Hannah I. Bishop

    2018-01-01

    Full Text Available Voltage-gated K+ (Kv channels play important roles in regulating neuronal excitability. Kv channels comprise four principal α subunits, and transmembrane and/or cytoplasmic auxiliary subunits that modify diverse aspects of channel function. AMIGO-1, which mediates homophilic cell adhesion underlying neurite outgrowth and fasciculation during development, has recently been shown to be an auxiliary subunit of adult brain Kv2.1-containing Kv channels. We show that AMIGO-1 is extensively colocalized with both Kv2.1 and its paralog Kv2.2 in brain neurons across diverse mammals, and that in adult brain, there is no apparent population of AMIGO-1 outside of that colocalized with these Kv2 α subunits. AMIGO-1 is coclustered with Kv2 α subunits at specific plasma membrane (PM sites associated with hypolemmal subsurface cisternae at neuronal ER:PM junctions. This distinct PM clustering of AMIGO-1 is not observed in brain neurons of mice lacking Kv2 α subunit expression. Moreover, in heterologous cells, coexpression of either Kv2.1 or Kv2.2 is sufficient to drive clustering of the otherwise uniformly expressed AMIGO-1. Kv2 α subunit coexpression also increases biosynthetic intracellular trafficking and PM expression of AMIGO-1 in heterologous cells, and analyses of Kv2.1 and Kv2.2 knockout mice show selective loss of AMIGO-1 expression and localization in neurons lacking the respective Kv2 α subunit. Together, these data suggest that in mammalian brain neurons, AMIGO-1 is exclusively associated with Kv2 α subunits, and that Kv2 α subunits are obligatory in determining the correct pattern of AMIGO-1 expression, PM trafficking and clustering.

  8. miR-1 is increased in pulmonary hypertension and downregulates Kv1.5 channels in rat pulmonary arteries.

    Science.gov (United States)

    Mondejar-Parreño, Gema; Callejo, María; Barreira, Bianca; Morales-Cano, Daniel; Esquivel-Ruiz, Sergio; Moreno, Laura; Cogolludo, Angel; Perez-Vizcaino, Francisco

    2018-05-02

    ■The expression of miR-1 is increased in lungs from the Hyp/Su5416 PAH rat model. ■PASMC from this animal model are more depolarised and show decreased expression and activity of Kv1.5. ■miR-1 directly targets Kv1.5 channels, reduces Kv1.5 activity and induces membrane depolarization. ■Antagomir-1 prevents Kv1.5 channel downregulation and the depolarization induced by hypoxia/Su5416 exposition. Impairment of voltage-dependent potassium channel (Kv) plays a central role in the development of cardiovascular diseases, including pulmonary arterial hypertension (PAH). MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the 3'-UTR region of specific mRNAs. The aim of this study was to analyze the effects of miR-1 on Kv channel function in pulmonary arteries (PA). Kv channel activity was studied in PA from healthy animals transfected with miR-1 or scrambled-miR. Kv currents were studied using the whole-cell configuration of patch-clamp technique. The characterization of the Kv1.5 currents was performed with the selective inhibitor DPO-1. miR-1 expression was increased and Kv1.5 channels were decreased in lungs from a rat model of PAH induced by hypoxia and Su5416. miR-1 transfection increased cell capacitance, reduced Kv1.5 currents and induced membrane depolarization in isolated pulmonary artery smooth muscle cells (PASMCs). Luciferase reporter assay indicated that KCNA5, which encodes Kv1.5 channels, is a direct target gene of miR-1. Incubation of PA with Su5416 and hypoxia (3% O 2 ) increased miR-1 and induced a decline in Kv1.5 currents, which was prevented by antagomiR-1. In conclusion, these data indicate that miR-1 induces PASMC hypertrophy and reduces the activity and expression of Kv channels, suggesting a pathophysiological role in PAH. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  9. Tööotsija töötuks tunnistamise ning töötu abiraha määramise ja maksmise ajutise juhendi kinnitamise kohta

    Index Scriptorium Estoniae

    1991-01-01

    Kehtib 1. aprillist 1991. Lisatud ajutine juhend: töö vahendamine tööotsijale, töötule, töötu abiraha määramine ja maksmine Lisatud: töötu abiraha arvutamine. Muudatus: RT I 1992 nr. 27 art. 362

  10. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination...

  11. Elongation factor Ts directly facilitates the formation and disassembly of the Escherichia coli elongation factor Tu·GTP·aminoacyl-tRNA ternary complex.

    Science.gov (United States)

    Burnett, Benjamin J; Altman, Roger B; Ferrao, Ryan; Alejo, Jose L; Kaur, Navdep; Kanji, Joshua; Blanchard, Scott C

    2013-05-10

    Aminoacyl-tRNA (aa-tRNA) enters the ribosome in a ternary complex with the G-protein elongation factor Tu (EF-Tu) and GTP. EF-Tu·GTP·aa-tRNA ternary complex formation and decay rates are accelerated in the presence of the nucleotide exchange factor elongation factor Ts (EF-Ts). EF-Ts directly facilitates the formation and disassociation of ternary complex. This system demonstrates a novel function of EF-Ts. Aminoacyl-tRNA enters the translating ribosome in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here, we describe bulk steady state and pre-steady state fluorescence methods that enabled us to quantitatively explore the kinetic features of Escherichia coli ternary complex formation and decay. The data obtained suggest that both processes are controlled by a nucleotide-dependent, rate-determining conformational change in EF-Tu. Unexpectedly, we found that this conformational change is accelerated by elongation factor Ts (EF-Ts), the guanosine nucleotide exchange factor for EF-Tu. Notably, EF-Ts attenuates the affinity of EF-Tu for GTP and destabilizes ternary complex in the presence of non-hydrolyzable GTP analogs. These results suggest that EF-Ts serves an unanticipated role in the cell of actively regulating the abundance and stability of ternary complex in a manner that contributes to rapid and faithful protein synthesis.

  12. Molecular Expression and Pharmacological Evidence for a Functional Role of Kv7 Channel Subtypes in Guinea Pig Urinary Bladder Smooth Muscle

    Science.gov (United States)

    Afeli, Serge A. Y.; Malysz, John; Petkov, Georgi V.

    2013-01-01

    Voltage-gated Kv7 (KCNQ) channels are emerging as essential regulators of smooth muscle excitability and contractility. However, their physiological role in detrusor smooth muscle (DSM) remains to be elucidated. Here, we explored the molecular expression and function of Kv7 channel subtypes in guinea pig DSM by RT-PCR, qRT-PCR, immunohistochemistry, electrophysiology, and isometric tension recordings. In whole DSM tissue, mRNAs for all Kv7 channel subtypes were detected in a rank order: Kv7.1~Kv7.2Kv7.3~Kv7.5Kv7.4. In contrast, freshly-isolated DSM cells showed mRNA expression of: Kv7.1~Kv7.2Kv7.5Kv7.3~Kv7.4. Immunohistochemical confocal microscopy analyses of DSM, conducted by using co-labeling of Kv7 channel subtype-specific antibodies and α-smooth muscle actin, detected protein expression for all Kv7 channel subtypes, except for the Kv7.4, in DSM cells. L-364373 (R-L3), a Kv7.1 channel activator, and retigabine, a Kv7.2-7.5 channel activator, inhibited spontaneous phasic contractions and the 10-Hz electrical field stimulation (EFS)-induced contractions of DSM isolated strips. Linopiridine and XE991, two pan-Kv7 (effective at Kv7.1-Kv7.5 subtypes) channel inhibitors, had opposite effects increasing DSM spontaneous phasic and 10 Hz EFS-induced contractions. EFS-induced DSM contractions generated by a wide range of stimulation frequencies were decreased by L-364373 (10 µM) or retigabine (10 µM), and increased by XE991 (10 µM). Retigabine (10 µM) induced hyperpolarization and inhibited spontaneous action potentials in freshly-isolated DSM cells. In summary, Kv7 channel subtypes are expressed at mRNA and protein levels in guinea pig DSM cells. Their pharmacological modulation can control DSM contractility and excitability; therefore, Kv7 channel subtypes provide potential novel therapeutic targets for urinary bladder dysfunction. PMID:24073284

  13. A charge-pump 60kV modulator for the ISOLDE target extraction voltage

    CERN Document Server

    Barlow, R A; Fowler, A; Gaudillet, H; Gharsa, T; Schipper, J

    2015-01-01

    The ISOLDE facility at CERN provides radioactive ion beams to a number of experimental stations. These ions are produced by a metal target, floating at 60 kV, which is impacted by a 1.4 GeV high intensity proton beam. The ions are then accelerated by a grounded extraction electrode to 60 keV, before transport to the experimental area. During proton beam impact extremely high ionisation of the volume around the target gives rise to significant leakage current which results in loss of charge on the effective target capacitance of approximately 6 nF. If short life-time isotopes are to be studied, the 60 kV must be re-established within a maximum of 10 ms. Recharging the target capacitance to 60 kV and to the required stability of better than 10-4 precludes a direct charging system and an alternative method of re-establishing the 60 kV is used. The present system [1], in operation since 1991, employs a resonant circuit which is triggered 35 µs prior to beam impact. This circuit transfers the charge on the effec...

  14. Workers exposure to electric fields in 400 kV substations and overhead line works

    International Nuclear Information System (INIS)

    Elovaara, J.; Kuisti, H.; Korpinen, L.

    2010-01-01

    The maximum exposing electric field strength magnitude in the highly inhomogeneous 400 kV electric field under work conditions can reach the value of several tens of kV/m. In spite of this the average current density is, in Finland, always lower than 10 mA/m 2 . Furthermore, the total body currents or contact currents are in 400 kV works clearly less than the limit value proposed by the EU's draft for Directive. On the basis of the obtained results it can be concluded that from the limit value point of view it is not necessary to take further actions to reduce the exposure of the workers when they are working in 400 kV substations and on 400 kV overhead lines. However, because the transient peak values of the contact current can be painful and disturb work, it is advisable to develop a safe and reliable method to connect the worker and the work object in the common ground (equipotential bonding). (authors)

  15. Modellering og måling af metan-emission fra kvæg

    DEFF Research Database (Denmark)

    Storm, I; Hellwing, Anne Louise Frydendahl; Nielsen, N I

    2011-01-01

    Udvikling af og kendskab til de metoder, der bruges til måling af kvægs metan-emission udgør en vigtig del af forskningen indenfor området. I dette indlæg præsenteres de mest anvendte metoder til måling og estimation af kvægs metan-emission, herunder modeller, in vitro inkubation, kammer-metoder,......-metoder, SF6-metoden og CO2-metoden. De enkelte metoder beskrives og fordele, ulemper og anvendelsesområder sammenlignes. Dette er vigtig baggrundsinformation for forståelse og fortolkning af øvrige forskningsresultater og for at kunne planlægge fremtidige projekter.......Udvikling af og kendskab til de metoder, der bruges til måling af kvægs metan-emission udgør en vigtig del af forskningen indenfor området. I dette indlæg præsenteres de mest anvendte metoder til måling og estimation af kvægs metan-emission, herunder modeller, in vitro inkubation, kammer...

  16. A capacitive level shifter for high voltage (2.5kV)

    DEFF Research Database (Denmark)

    Andersen, Thomas; Andersen, Michael A. E.; Thomsen, Ole Cornelius

    2012-01-01

    with focus on low power consumption as well as low capacitive load between the floating half-bridge node and ground (output capacitance). The operation of the level-shifter is tested and verified by measurements on a prototype half-bridge gate driver. Results conclude stabile operation at 2.44kV, 50k...

  17. Differential expression of KvLQT1 and its regulator IsK in mouse epithelia

    NARCIS (Netherlands)

    Demolombe, S.; Franco, D.; de Boer, P.; Kuperschmidt, S.; Roden, D.; Pereon, Y.; Jarry, A.; Moorman, A. F.; Escande, D.

    2001-01-01

    KCNQ1 is the human gene responsible in most cases for the long QT syndrome, a genetic disorder characterized by anomalies in cardiac repolarization leading to arrhythmias and sudden death. KCNQ1 encodes a pore-forming K+ channel subunit termed KvLQT1 which, in association with its regulatory

  18. Effects of electromagnetic field of 33 and 275 kV influences on ...

    African Journals Online (AJOL)

    The effects of electromagnetic fields (EMF) from 33 and 275 kV high voltage transmission line on biochemical and antioxidant system changes in mustard leaf (Brassica chinensis) were investigated under field condition. Mustard leaves were exposed to EMF from power lines at distances of 0, 3, 6, 9, 10, 12, 15, 18, 20, 21, ...

  19. A neutral grounding metallic resistor failure in a 35 kV network

    Directory of Open Access Journals (Sweden)

    Simić Ninoslav

    2011-01-01

    Full Text Available This paper presents the results of observations and measurements of the impedance of the metal resistor for grounding neutral of the 35 kV network, before and after damaging event. The proposed measures are to be taken in order to eliminate a failure in this particular case, as well as the prevention of similar events.

  20. Interlaboratory comparison of measuring results of magnetic field near 400 kV overhead power line

    Directory of Open Access Journals (Sweden)

    Grbić Maja

    2012-01-01

    Full Text Available The paper presents a comparison of measured results of magnetic field near 400 kV overhead power lines obtained by three laboratories. This interlaboratory comparison was performed to ensure confidence in the quality of the test results. The measured results were analyzed with standard methods, using En number, based on which the evaluation of the laboratories was performed.

  1. Design and performance of a 30 KV electron gun with ten independent cathodes & a magnetic lens.

    Energy Technology Data Exchange (ETDEWEB)

    Rudys, Joseph Matthew; Reed, Kim Warren

    2006-08-01

    Measurements on a 30 kV electron gun with ten independent cathodes, operating in a 6.5 Tesla (T) magnetic field are presented. An earlier paper covered the design of this electron gun [1]. Experimental results are compared to model predictions. Beam current is compared to theoretical space charge limited flow.

  2. Udbredelse af den baktielle zoonose i danske kvægbesætninger

    DEFF Research Database (Denmark)

    Bødker, Rene; Christoffersen, Anna-Bodil

    2008-01-01

    Tankmælksprøver fra 742 mælkeleverende kvægejendomme blev analyseret for antistoffer mod Coxiella burnetii i en ELISA. 57% af ejendommene havde positive prøver i den serologiske test. Prøverne var diagnostiske indsendelser til DTU Veterinærinstituttet og udgjorde derfor ikke en repræsentativ...

  3. Independent and cooperative motions of the Kv1.2 channel: voltage sensing and gating.

    Science.gov (United States)

    Yeheskel, Adva; Haliloglu, Turkan; Ben-Tal, Nir

    2010-05-19

    Voltage-gated potassium (Kv) channels, such as Kv1.2, are involved in the generation and propagation of action potentials. The Kv channel is a homotetramer, and each monomer is composed of a voltage-sensing domain (VSD) and a pore domain (PD). We analyzed the fluctuations of a model structure of Kv1.2 using elastic network models. The analysis suggested a network of coupled fluctuations of eight rigid structural units and seven hinges that may control the transition between the active and inactive states of the channel. For the most part, the network is composed of amino acids that are known to affect channel activity. The results suggested allosteric interactions and cooperativity between the subunits in the coupling between the motion of the VSD and the selectivity filter of the PD, in accordance with recent empirical data. There are no direct contacts between the VSDs of the four subunits, and the contacts between these and the PDs are loose, suggesting that the VSDs are capable of functioning independently. Indeed, they manifest many inherent fluctuations that are decoupled from the rest of the structure. In general, the analysis suggests that the two domains contribute to the channel function both individually and cooperatively. Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  4. Measurements of Voltage Harmonics in 400 kV Transmission Network

    Directory of Open Access Journals (Sweden)

    Ryszard Pawełek

    2014-06-01

    Full Text Available The paper deals with the analysis of voltage harmonics measurements performed in the 400 kV transmission network. The voltage was measured by means of three transducers: resistive voltage divider, inductive measuring transformer and capacitive voltage measuring transformer. Instrument errors were estimated for measuring transformers with reference to the harmonic values obtained from the voltage divider.

  5. Modification of 300kV RF Ion Source for 1-MV Electrostatic Accelerator at KOMAC

    International Nuclear Information System (INIS)

    Kim, Dae-Il; Kwon, Hyeok-Jung; Park, Sae-Hoon; Cho, Yong-Sub

    2015-01-01

    The specifications of the 1-MV electrostatic accelerator are shown as below. High voltage power supply is electron transformer rectifier (ELV) type which was developed in Nuclear Physics Institute (Novosibirsk) for industrial electron accelerators. And accelerator column consists of alumina and metal electrode rings were 0.5m-long brazed structure which can be installed horizontally. In case of ion source for 1-MV electrostatic accelerator, it is chosen a thonemann type rf ion source and 300-kV test-stand was made up to confirm the stable operating conditions. High voltage power supply is fabricated by domestic company, and its operation has been confirming at KOMAC site. Equally, the ion source of 300-kV test-stand should be modified to install into the high voltage power supply. In this paper, modification of ion source of 300-kV test-stand for 1-MV electrostatic accelerator is presented and its processes are considered. 300-kV RF ion source and power supply are testing for the 1-MV electrostatic accelerator and trying for combination between them. The 1-MV electrostatic accelerator will be fabricated with domestic companies and tested in the beam application research building at KOMAC

  6. Modification of 300kV RF Ion Source for 1-MV Electrostatic Accelerator at KOMAC

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae-Il; Kwon, Hyeok-Jung; Park, Sae-Hoon; Cho, Yong-Sub [KOMAC, Gyeongju (Korea, Republic of)

    2015-05-15

    The specifications of the 1-MV electrostatic accelerator are shown as below. High voltage power supply is electron transformer rectifier (ELV) type which was developed in Nuclear Physics Institute (Novosibirsk) for industrial electron accelerators. And accelerator column consists of alumina and metal electrode rings were 0.5m-long brazed structure which can be installed horizontally. In case of ion source for 1-MV electrostatic accelerator, it is chosen a thonemann type rf ion source and 300-kV test-stand was made up to confirm the stable operating conditions. High voltage power supply is fabricated by domestic company, and its operation has been confirming at KOMAC site. Equally, the ion source of 300-kV test-stand should be modified to install into the high voltage power supply. In this paper, modification of ion source of 300-kV test-stand for 1-MV electrostatic accelerator is presented and its processes are considered. 300-kV RF ion source and power supply are testing for the 1-MV electrostatic accelerator and trying for combination between them. The 1-MV electrostatic accelerator will be fabricated with domestic companies and tested in the beam application research building at KOMAC.

  7. Beam Extraction for 1-MV Electrostatic Accelerator at the 300 kV Test Stand

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sae-Hoon; Kim, Yu-Seok [Dongguk University, Seoul (Korea, Republic of); Kwon, Hyeok-Jung; Cho, Yong-Sub [KOMAC, Gyeongju (Korea, Republic of)

    2015-05-15

    The Korea Multipurpose Accelerator Complex (KOMAC) has been developing a 300-kV test stand for a 1-MV electrostatic accelerator ion source. The ion source in the high-pressure vessel is required to have a high reliability. The test stand has been proposed and developed to confirm the stable operating conditions of the ion source. The ion source will be tested at the test stand to verify the long-time operating conditions. The test stand comprises a 300-kV high-voltage terminal, a battery for the ion-source power, a 60-Hz inverter, 200-MHz RF power, a 5-kV extraction power supply, a 300-kV accelerating tube, and a vacuum system. A beam extraction experiment for the test stand was performed, and the beam current was measured using a faraday cup in the chamber. A beam extraction results for the RF ion source will be presented. Beam extraction from the RF ion source of the test stand is verified by measuring the beam current with a faraday cup in the chamber. Thus far NI Labview, PLC and faraday cup have been used to measure the beam current. The OPC server is useful for monitoring the PLC values. The average beam current of (a), (b) and (c) shown in figure 2 are 110.241µA, 105.8597µA and 103.5278µA respectively.

  8. The 150 kV cp microfocus X-ray unit

    NARCIS (Netherlands)

    Fontijn, L.A.

    1979-01-01

    Development of microfocus X-ray technique is defined. Advantages on other methods, principle of operation and the material comprising an intense electron source imaged on an X-ray target by means of a double magnetic lense system, are described. Resolution value at 150 kV is the imaging of a 0.1 mm

  9. A fast 30 kV 5 kHz repetition rate resonant capacitor charger

    NARCIS (Netherlands)

    Beckers, F.J.C.M.; Huiskamp, T.; van Heesch, E.J.M.; Pemen, A.J.M.

    2016-01-01

    A novel circuit topology of a fast 30 kV resonant capacitor charger is presented in this paper. The charger is designed for high repetition rate spark gap based pulsed power modulators. A spark gap can fire spontaneously (pre-firing) during charging of a capacitor bank due to poor dielectric

  10. Characterisation of 10 kV 10 A SiC MOSFET

    DEFF Research Database (Denmark)

    Eni, Emanuel-Petre; Incau, Bogdan Ioan; Munk-Nielsen, Stig

    2015-01-01

    The objective of this paper is to characterize and evaluate the static and dynamic performances of 10 kV 10 A 4H-SIC MOSFETs at high temperatures. The results show good electrical performances of the SiC MOSFETs for high temperature operations. The double-pulse test results showed interesting...

  11. 10kV SiC MOSFET split output power module

    DEFF Research Database (Denmark)

    Beczkowski, Szymon; Li, Helong; Uhrenfeldt, Christian

    2015-01-01

    The poor body diode performance of the first generation of 10kV SiC MOSFETs and the parasitic turn-on phenomenon limit the performance of SiC based converters. Both these problems can potentially be mitigated using a split output topology. In this paper we present a comparison between a classical...

  12. harmonic load modeling: a case study of 33 kv abuja steel mill feeder

    African Journals Online (AJOL)

    HOD

    An in-depth study of the harmonic orders inherent in a power system network is required ... This paper studied the harmonic orders of the 33 kV Abuja Steel Feeder .... models for various industrial and household electrical ..... Malaysia, 2013.

  13. Reliability assessment of Port Harcourt 33/11kv Distribution System ...

    African Journals Online (AJOL)

    This makes reliability studies an important task besides all the other analyses required for assessing the system performance. The paper presents an analytical approach in the reliability assessment of the Port Harcourt 33/11kV power distribution system. The assessment was performed with the 2009 power outage data ...

  14. K významnému jubileu Květy Sgallové

    Czech Academy of Sciences Publication Activity Database

    Ibrahim, Robert

    2009-01-01

    Roč. 57, č. 4 (2009), s. 608-616 ISSN 0009-0468 Institutional research plan: CEZ:AV0Z90560517 Keywords : Sgallová, Květa * Czech literature * 19th century Subject RIV: AJ - Letters, Mass-media, Audiovision

  15. Full Scale Experimental Study on the Air Insulation of 765kV Transmission Lines

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jong Boo; Shin, Jeong Woon; Cho, Sung Bae; Chang, Sung Chil; Kwak, Joo Shik [Korea Electric Power Research Institute, Taejon (Korea, Republic of); Kim, Ik Soo; Lee, Heyoung Ho; Kang, Young Sik; Lee, Hong Sik; Choi, Beyoung Joo; Kim, Young Bae [Korea Electrotechnology Research Institute, Changwon (Korea, Republic of)

    1997-12-31

    Design and construction of testing yard. Technical specification of impulse voltage generator which was installed in testing yard. Corrosion and statistical analysis of test data. Test object setup and test results of flashover characteristic in various paths. Recommended optimum air clearance of 765kV transmission lines. (author). 163 refs., figs.

  16. Pivoting between calmodulin lobes triggered by calcium in the Kv7.2/calmodulin complex.

    Science.gov (United States)

    Alaimo, Alessandro; Alberdi, Araitz; Gomis-Perez, Carolina; Fernández-Orth, Juncal; Bernardo-Seisdedos, Ganeko; Malo, Covadonga; Millet, Oscar; Areso, Pilar; Villarroel, Alvaro

    2014-01-01

    Kv7.2 (KCNQ2) is the principal molecular component of the slow voltage gated M-channel, which strongly influences neuronal excitability. Calmodulin (CaM) binds to two intracellular C-terminal segments of Kv7.2 channels, helices A and B, and it is required for exit from the endoplasmic reticulum. However, the molecular mechanisms by which CaM controls channel trafficking are currently unknown. Here we used two complementary approaches to explore the molecular events underlying the association between CaM and Kv7.2 and their regulation by Ca(2+). First, we performed a fluorometric assay using dansylated calmodulin (D-CaM) to characterize the interaction of its individual lobes to the Kv7.2 CaM binding site (Q2AB). Second, we explored the association of Q2AB with CaM by NMR spectroscopy, using (15)N-labeled CaM as a reporter. The combined data highlight the interdependency of the N- and C-lobes of CaM in the interaction with Q2AB, suggesting that when CaM binds Ca(2+) the binding interface pivots between the N-lobe whose interactions are dominated by helix B and the C-lobe where the predominant interaction is with helix A. In addition, Ca(2+) makes CaM binding to Q2AB more difficult and, reciprocally, the channel weakens the association of CaM with Ca(2+).

  17. Pivoting between calmodulin lobes triggered by calcium in the Kv7.2/calmodulin complex.

    Directory of Open Access Journals (Sweden)

    Alessandro Alaimo

    Full Text Available Kv7.2 (KCNQ2 is the principal molecular component of the slow voltage gated M-channel, which strongly influences neuronal excitability. Calmodulin (CaM binds to two intracellular C-terminal segments of Kv7.2 channels, helices A and B, and it is required for exit from the endoplasmic reticulum. However, the molecular mechanisms by which CaM controls channel trafficking are currently unknown. Here we used two complementary approaches to explore the molecular events underlying the association between CaM and Kv7.2 and their regulation by Ca(2+. First, we performed a fluorometric assay using dansylated calmodulin (D-CaM to characterize the interaction of its individual lobes to the Kv7.2 CaM binding site (Q2AB. Second, we explored the association of Q2AB with CaM by NMR spectroscopy, using (15N-labeled CaM as a reporter. The combined data highlight the interdependency of the N- and C-lobes of CaM in the interaction with Q2AB, suggesting that when CaM binds Ca(2+ the binding interface pivots between the N-lobe whose interactions are dominated by helix B and the C-lobe where the predominant interaction is with helix A. In addition, Ca(2+ makes CaM binding to Q2AB more difficult and, reciprocally, the channel weakens the association of CaM with Ca(2+.

  18. Electronics system for the 150 kV negative ion test stand at BNL

    International Nuclear Information System (INIS)

    Larson, R.A.

    1977-01-01

    The 150 kV test stand at BNL is being used to investigate the extraction, acceleration and transport problems associated with the development of intense negative ion beams. The power supplies associated with these functions as well as the control and monitoring electronics are described

  19. Structural basis for KV7.1/KCNEx interactions in the IKs channel complex

    DEFF Research Database (Denmark)

    Lundby, Alicia; Tseng, Gea-Ny; Schmitt, Nicole

    2010-01-01

    The cardiac I(Ks) current is involved in action potential repolarization, where its primary function is to limit action potential prolongation during sympathetic stimulation. The I(Ks) channel is mainly composed of K(V)7.1 ion channels associated with KCNE1 auxiliary subunits. The availability of...

  20. Axon initial segment Kv1 channels control axonal action potential waveform and synaptic efficacy

    NARCIS (Netherlands)

    Kole, Maarten H. P.; Letzkus, Johannes J.; Stuart, Greg J.

    2007-01-01

    Action potentials are binary signals that transmit information via their rate and temporal pattern. In this context, the axon is thought of as a transmission line, devoid of a role in neuronal computation. Here, we show a highly localized role of axonal Kv1 potassium channels in shaping the action

  1. The Pandora multi-algorithm approach to automated pattern recognition of cosmic-ray muon and neutrino events in the MicroBooNE detector

    CERN Document Server

    Acciarri, R.; An, R.; Anthony, J.; Asaadi, J.; Auger, M.; Bagby, L.; Balasubramanian, S.; Baller, B.; Barnes, C.; Barr, G.; Bass, M.; Bay, F.; Bishai, M.; Blake, A.; Bolton, T.; Camilleri, L.; Caratelli, D.; Carls, B.; Castillo Fernandez, R.; Cavanna, F.; Chen, H.; Church, E.; Cianci, D.; Cohen, E.; Collin, G. H.; Conrad, J. M.; Convery, M.; Crespo-Anadón, J. I.; Del Tutto, M.; Devitt, D.; Dytman, S.; Eberly, B.; Ereditato, A.; Escudero Sanchez, L.; Esquivel, J.; Fadeeva, A. A.; Fleming, B. T.; Foreman, W.; Furmanski, A. P.; Garcia-Gamez, D.; Garvey, G. T.; Genty, V.; Goeldi, D.; Gollapinni, S.; Graf, N.; Gramellini, E.; Greenlee, H.; Grosso, R.; Guenette, R.; Hackenburg, A.; Hamilton, P.; Hen, O.; Hewes, J.; Hill, C.; Ho, J.; Horton-Smith, G.; Hourlier, A.; Huang, E.-C.; James, C.; Jan de Vries, J.; Jen, C.-M.; Jiang, L.; Johnson, R. A.; Joshi, J.; Jostlein, H.; Kaleko, D.; Karagiorgi, G.; Ketchum, W.; Kirby, B.; Kirby, M.; Kobilarcik, T.; Kreslo, I.; Laube, A.; Li, Y.; Lister, A.; Littlejohn, B. R.; Lockwitz, S.; Lorca, D.; Louis, W. C.; Luethi, M.; Lundberg, B.; Luo, X.; Marchionni, A.; Mariani, C.; Marshall, J.; Martinez Caicedo, D. A.; Meddage, V.; Miceli, T.; Mills, G. B.; Moon, J.; Mooney, M.; Moore, C. D.; Mousseau, J.; Murrells, R.; Naples, D.; Nienaber, P.; Nowak, J.; Palamara, O.; Paolone, V.; Papavassiliou, V.; Pate, S. F.; Pavlovic, Z.; Piasetzky, E.; Porzio, D.; Pulliam, G.; Qian, X.; Raaf, J. L.; Rafique, A.; Rochester, L.; Rudolf von Rohr, C.; Russell, B.; Schmitz, D. W.; Schukraft, A.; Seligman, W.; Shaevitz, M. H.; Sinclair, J.; Smith, A.; Snider, E. L.; Soderberg, M.; Söldner-Rembold, S.; Soleti, S. R.; Spentzouris, P.; Spitz, J.; St. John, J.; Strauss, T.; Szelc, A. M.; Tagg, N.; Terao, K.; Thomson, M.; Toups, M.; Tsai, Y.-T.; Tufanli, S.; Usher, T.; Van De Pontseele, W.; Van de Water, R. G.; Viren, B.; Weber, M.; Wickremasinghe, D. A.; Wolbers, S.; Wongjirad, T.; Woodruff, K.; Yang, T.; Yates, L.; Zeller, G. P.; Zennamo, J.; Zhang, C.

    2017-01-01

    The development and operation of Liquid-Argon Time-Projection Chambers for neutrino physics has created a need for new approaches to pattern recognition in order to fully exploit the imaging capabilities offered by this technology. Whereas the human brain can excel at identifying features in the recorded events, it is a significant challenge to develop an automated, algorithmic solution. The Pandora Software Development Kit provides functionality to aid the design and implementation of pattern-recognition algorithms. It promotes the use of a multi-algorithm approach to pattern recognition, in which individual algorithms each address a specific task in a particular topology. Many tens of algorithms then carefully build up a picture of the event and, together, provide a robust automated pattern-recognition solution. This paper describes details of the chain of over one hundred Pandora algorithms and tools used to reconstruct cosmic-ray muon and neutrino events in the MicroBooNE detector. Metrics that assess the...

  2. Pharmacological Targeting Of Neuronal Kv7.2/3 Channels: A Focus On Chemotypes And Receptor Sites.

    Science.gov (United States)

    Miceli, Francesco; Soldovieri, Maria Virginia; Ambrosino, Paolo; Manocchio, Laura; Medoro, Alessandro; Mosca, Ilaria; Taglialatela, Maurizio

    2017-10-12

    The Kv7 (KCNQ) subfamily of voltage-gated potassium channels consists of 5 members (Kv7.1-5) each showing a characteristic tissue distribution and physiological roles. Given their functional heterogeneity, Kv7 channels represent important pharmacological targets for development of new drugs for neuronal, cardiac and metabolic diseases. In the present manuscript, we focus on describing the pharmacological relevance and the potential therapeutic applications of drugs acting on neuronally-expressed Kv7.2/3 channels, placing particular emphasis on the different modulator chemotypes, and highlighting their pharmacodynamic and, whenever possible, pharmacokinetic peculiarities. The present work is based on an in-depth search of the currently available scientific literature, and on our own experience and knowledge in the field of neuronal Kv7 channel pharmacology. Space limitations impeded to describe the full pharmacological potential of Kv7 channels; thus, we have chosen to focus on neuronal channels composed of Kv7.2 and Kv7.3 subunits, and to mainly concentrate on their involvement in epilepsy. An astonishing heterogeneity in the molecular scaffolds exploitable to develop Kv7.2/3 modulators is evident, with important structural/functional peculiarities of distinct compound classes. In the present work we have attempted to show the current status and growing potential of the Kv7 pharmacology field. We anticipate a bright future for the field, and we express our hopes that the efforts herein reviewed will result in an improved treatment of hyperexcitability (or any other) diseases. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  3. Radiation dose reduction sinogram affirmed iterative reconstruction and automatic tube voltage modulation(CARE kV) in abdominal CT

    International Nuclear Information System (INIS)

    Shin, Hyun Joo; Chung, Yong Eun; Lee, Young Han; Choi, Jin Young; Park, Mi Suk; Kim, Myeong Jin; Kim, Ki Whang

    2013-01-01

    To evaluate the feasibility of sinogram-affirmed iterative reconstruction (SAFIRE) and automated kV modulation (CARE kV) in reducing radiation dose without increasing image noise for abdominal CT examination. This retrospective study included 77 patients who received CT imaging with an application of CARE kV with or without SAFIRE and who had comparable previous CT images obtained without CARE kV or SAFIRE, using the standard dose (i.e., reference mAs of 240) on an identical CT scanner and reconstructed with filtered back projection (FBP) within 1 year. Patients were divided into two groups: group A (33 patients, CT scanned with CARE kV); and group B (44 patients, scanned after reducing the reference mAs from 240 to 170 and applying both CARE kV and SAFIRE). CT number, image noise for four organs and radiation dose were compared among the two groups. Image noise increased after CARE kV application (p < 0.001) and significantly decreased as SAFIRE strength increased (p < 0.001). Image noise with reduced-mAs scan (170 mAs) in group B became similar to that of standard-dose FBP images after applying CARE kV and SAFIRE strengths of 3 or 4 when measured in the aorta, liver or muscle (p ≥ 0.108). Effective doses decreased by 19.4% and 41.3% for groups A and B, respectively (all, p < 0.001) after application of CARE kV with or without SAFIRE. Combining CARE kV, reduction of mAs from 240 to 170 mAs and noise reduction by applying SAFIRE strength 3 or 4 reduced the radiation dose by 41.3% without increasing image noise compared with the standard-dose FBP images.

  4. Mutation of the conserved Gly94 and Gly126 in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Kjaersgård, I V; Wiborg, O

    1995-01-01

    All guanine-nucleotide-binding proteins cycle between an inactive, GDP-bound and an active, GTP-bound conformation whereby they function as molecular switches. Elongation factor Tu from Escherichia coli is used as a model for defining residues important in the switch mechanism. Gly94 and Gly126...... were separately mutated to alanine residues to study their role in the switch mechanism. The mutant proteins are denoted [G94A]EF-Tu and [G126A]EF-Tu, respectively. Both mutations affect the affinities for guanine nucleotides considerably, resulting in a decrease in the characteristic preference...... for GDP over GTP. Furthermore the [G94A]EF-Tu mutant possesses an increased GTPase activity. The aminoacyl-tRNA affinity is much reduced for [G94A]EF-Tu, as reflected in an increase of the dissociation rate constant for the ternary complex by a factor of 40. Surprisingly, however, both mutants...

  5. Elongation Factor Ts Directly Facilitates the Formation and Disassembly of the Escherichia coli Elongation Factor Tu·GTP·Aminoacyl-tRNA Ternary Complex*

    Science.gov (United States)

    Burnett, Benjamin J.; Altman, Roger B.; Ferrao, Ryan; Alejo, Jose L.; Kaur, Navdep; Kanji, Joshua; Blanchard, Scott C.

    2013-01-01

    Aminoacyl-tRNA enters the translating ribosome in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here, we describe bulk steady state and pre-steady state fluorescence methods that enabled us to quantitatively explore the kinetic features of Escherichia coli ternary complex formation and decay. The data obtained suggest that both processes are controlled by a nucleotide-dependent, rate-determining conformational change in EF-Tu. Unexpectedly, we found that this conformational change is accelerated by elongation factor Ts (EF-Ts), the guanosine nucleotide exchange factor for EF-Tu. Notably, EF-Ts attenuates the affinity of EF-Tu for GTP and destabilizes ternary complex in the presence of non-hydrolyzable GTP analogs. These results suggest that EF-Ts serves an unanticipated role in the cell of actively regulating the abundance and stability of ternary complex in a manner that contributes to rapid and faithful protein synthesis. PMID:23539628

  6. Combined kV and MV imaging for real-time tracking of implanted fiducial markers

    International Nuclear Information System (INIS)

    Wiersma, R. D.; Mao Weihua; Xing, L.

    2008-01-01

    In the presence of intrafraction organ motion, target localization uncertainty can greatly hamper the advantage of highly conformal dose techniques such as intensity modulated radiation therapy (IMRT). To minimize the adverse dosimetric effect caused by tumor motion, a real-time knowledge of the tumor position is required throughout the beam delivery process. The recent integration of onboard kV diagnostic imaging together with MV electronic portal imaging devices on linear accelerators can allow for real-time three-dimensional (3D) tumor position monitoring during a treatment delivery. The aim of this study is to demonstrate a near real-time 3D internal fiducial tracking system based on the combined use of kV and MV imaging. A commercially available radiotherapy system equipped with both kV and MV imaging systems was used in this work. A hardware video frame grabber was used to capture both kV and MV video streams simultaneously through independent video channels at 30 frames per second. The fiducial locations were extracted from the kV and MV images using a software tool. The geometric tracking capabilities of the system were evaluated using a pelvic phantom with embedded fiducials placed on a moveable stage. The maximum tracking speed of the kV/MV system is approximately 9 Hz, which is primarily limited by the frame rate of the MV imager. The geometric accuracy of the system is found to be on the order of less than 1 mm in all three spatial dimensions. The technique requires minimal hardware modification and is potentially useful for image-guided radiation therapy systems

  7. KV4.3 N-terminal deletion mutant Δ2–39

    Science.gov (United States)

    Hovind, Laura J; Skerritt, Matthew R

    2011-01-01

    Gating transitions in the KV4.3 N-terminal deletion mutant Δ2–39 were characterized in the absence and presence of KChIP2b. We particularly focused on gating characteristics of macroscopic (open state) versus closed state inactivation (CSI) and recovery. In the absence of KChIP2b Δ2–39 did not significantly alter the steady-state activation “a4” relationship or general CSI characteristics, but it did slow the kinetics of deactivation, macroscopic inactivation and macroscopic recovery. Recovery kinetics (for both WT KV4.3 and Δ2–39) were complicated and displayed sigmoidicity, a process which was enhanced by Δ2–39. Deletion of the proximal N-terminal domain therefore appeared to specifically slow mechanisms involved in regulating gating transitions occurring after the channel open state(s) had been reached. In the presence of KChIP2b Δ2–39 recovery kinetics (from both macroscopic and CSI) were accelerated, with an apparent reduction in initial sigmoidicity. Hyperpolarizing shifts in both “a4” and isochronal inactivation “i” were also produced. KChIP2b-mediated remodeling of KV4.3 gating transitions was therefore not obligatorily dependent upon an intact N-terminus. To account for these effects we propose that KChIP2 regulatory domains exist in KV4.3 α subunit regions outside of the proximal N-terminal. In addition to regulating macroscopic inactivation, we also propose that the KV4.3 N-terminus may act as a novel regulator of deactivation-recovery coupling. PMID:21057209

  8. Constitutional and somatic methylation status of DMRH19 and KvDMR in Wilms tumor patients

    Directory of Open Access Journals (Sweden)

    Leila C.A. Cardoso

    2012-01-01

    Full Text Available The most frequent epigenetic alterations in Wilms tumor (WT occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19 that contains the IGF2 gene and an imprinted maternally expressed transcript (H19 and centromeric domain 2 (KvDMR that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19 was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples: 16/34 with isolated HM DMRH19 and 12/34 with concomitant HM DMRH19 and KvDMR hypomethylation, indicating paternal uniparental disomy. With the exception of one blood sample, the MS-MLPA and pyrosequencing findings were concordant. Diffuse or focal anaplasia was present in five tumor samples and was associated with isolated somatic HM DMRH19 in four of them. Constitutional 11p15 methylation abnormalities were present in 5% of the samples and somatic abnormalities in the majority of tumors. Combined analysis of DMRH19/KvDMR by pyrosequencing and MS-MLPA is beneficial for characterizing epigenetic anomalies in WT, and MS-MLPA is useful and reliable for estimation of DNA methylation in a clinical setting.

  9. Improvement of the 400 kV linac electron source of AmPS

    International Nuclear Information System (INIS)

    Kroes, F.B.; Beuzekom, M.G. van; Dobbe, N.J.; Es, J.T. van; Jansweijer, P.P.M.; Kruijer, A.H.; Luigjes, G.; Sluijk, T.G.B.

    1992-01-01

    The installation of the 900 MeV Amsterdam Pulse Stretcher is nearly completed and its commissioning will start Spring 1992. The existing linac MEA will inject electrons in the AmPS ring. The linacs peak current will be increased from 20 to 80 mA. This requires modification of the 400 kV low emittance gun which now will deliver a peak current of maximum 400 mA instead of 100 mA at a pulse width of 2.1 μsec. The fourfold increase of the peakcurrent is obtained by doubling both the gun perveance (new gun part) and the pulsed extractor voltage. After chopping and pre-bunching more than 80 mA will be available for acceleration in MEA. To obtain optimum beam quality over this increased current range the hot deck electronics, operating at -400 kV, has been exchanged by a state of the art fast high voltage FET switching supply. The increased space charge forces in the beam require stronger electro-static focusing in the first electrostatic gap to define the beam diameter at the gun exit. This is accomplished with a 25 kV controlled power supply. A build in microprocessor, coupled to the local computer by optical fibers, is used to monitor and control the gun parameters. The 5kV gun extractor voltage pulse shape can be monitored by means of an analog fibre transducer with build in calibration. Finally, in order to improve the energy stability of the accelerated electrons a serial electron-tube stabilizer was added to the 400 kV DC power supply. A supply stability of 2. 10 -5 has been achieved. (author). 4 refs.; 6 figs

  10. Touch and step potential analysis at 23.9kV to 4.16kV & 13.8kV to 4.16kV distribution substations with pad-mounted transformers, floating grounds, and other exposed ungrounded metal bodies using WinIGS

    Science.gov (United States)

    Guzman, David G.

    An electrical substation is composed of various subsystems that allow for the effective and safe operation of the power grid. One of the subsystems integrating a conventional substation is defined as the ground grid system. This system allows for the effective operation of the power grid and all the electrical equipment connected to it by providing a ground potential reference, commonly known as the system ground. In addition, the ground grid system provides safety to the workers and the public transiting inside or living nearby a substation by reducing the step and touch potential (or voltage) levels present during a system fault. In today's utility industry practices there is an increasing trend for using pad-mounted electrical equipment for substation applications in an effort to construct new or upgrade existing electrical facilities inside limited property spaces. This thesis work presents an analysis for the effects of touch and step voltages at existing distribution substations where 23.9kV to 4.16kV & 13.8kV to 4.16kV pad-mounted transformers and other pad-mounted switchgear was installed to replace the traditional station class equipment. Moreover, this study will expose modeling techniques employed to define and determine the effects of floating grounds and other exposed metal bodies inside or surrounding these substations using WinIGS; this is in an effort to determine any risks of electric shock associated with this type of installations. The results presented in this work are intended to verify the requirements for the ground grid analysis and design for 4.16kV distribution substations with pad-mounted equipment in order to prevent dangerous step and touch voltage levels appearing at these sites during system faults; and ultimately prevent exposing individuals to the risk of an electric shock.

  11. Kreativitāte un sajūtu meklēšana jauniešiem

    OpenAIRE

    Tarhanova, Jekaterina

    2013-01-01

    Pētījuma mērķis bija izpētīt kreativitāti (radošumu) un sajūtu meklēšanu jauniešu populācijā. Sakarā ar to, ka pētījumu veica, lai iegūtu bakalaura grādu psiholoģijā, izlases apjoms bija tikai 75 cilvēki. Izlase tika veidota no studentiem vecumā no 18 līdz 23 gadiem. Cilvēki izpildīja Sajūtu Meklēšanas Skalas (Sensation Seeking Scale) MVU modificēto variantu un radošuma testu Zīmējumu pabeigšana (Complete Figures), kas ir Torensa Kreatīvās Domāšanas Testa (Torrance Test of Creative Thin...

  12. Molecular insight into γ-γ tubulin lateral interactions within the γ-tubulin ring complex (γ-TuRC)

    Science.gov (United States)

    Suri, Charu; Hendrickson, Triscia W.; Joshi, Harish C.; Naik, Pradeep Kumar

    2014-09-01

    γ-tubulin is essential for the nucleation and organization of mitotic microtubules in dividing cells. It is localized at the microtubule organizing centers and mitotic spindle fibres. The most well accepted hypothesis for the initiation of microtubule polymerization is that α/β-tubulin dimers add onto a γ-tubulin ring complex (γTuRC), in which adjacent γ-tubulin subunits bind to the underlying non-tubulin components of the γTuRC. This template thus determines the resulting microtubule lattice. In this study we use molecular modelling and molecular dynamics simulations, combined with computational MM-PBSA/MM-GBSA methods, to determine the extent of the lateral atomic interaction between two adjacent γ-tubulins within the γTuRC. To do this we simulated a γ-γ homodimer for 10 ns and calculated the ensemble average of binding free energies of -107.76 kcal/mol by the MM-PBSA method and of -87.12 kcal/mol by the MM-GBSA method. These highly favourable binding free energy values imply robust lateral interactions between adjacent γ-tubulin subunits in addition to their end-interactions longitudinally with other proteins of γTuRC. Although the functional reconstitution of γ-TuRC subunits and their stepwise in vitro assembly from purified components is not yet feasible, we nevertheless wanted to recognize hotspot amino acids responsible for key γ-γ interactions. Our free energy decomposition data from converting a compendium of amino acid residues identified an array of hotspot amino acids. A subset of such mutants can be expressed in vivo in living yeast. Because γTuRC is important for the growth of yeast, we could test whether this subset of the hotspot mutations support growth of yeast. Consistent with our model, γ-tubulin mutants that fall into our identified hotspot do not support yeast growth.

  13. Kv4 channels underlie the subthreshold-operating A-type K+-current in nociceptive dorsal root ganglion neurons

    Directory of Open Access Journals (Sweden)

    Thanawath R Na Phuket

    2009-07-01

    Full Text Available The dorsal root ganglion (DRG contains heterogeneous populations of sensory neurons including primary nociceptive neurons and C-fibers implicated in pain signaling.  Recent studies have demonstrated DRG hyperexcitability associated with downregulation of A-type K+ channels; however, the molecular correlate of the corresponding A-type K+ current (IA has remained hypothetical.  Kv4 channels may underlie the IA in DRG neurons.  We combined electrophysiology, molecular biology (whole-tissue and single-cell RT-PCR and immunohistochemistry to investigate the molecular basis of the IA in acutely dissociated DRG neurons from 7-8 day-old rats.  Whole-cell recordings demonstrate a robust tetraethylammonium-resistant (20 mM and 4-aminopyridine-sensitive (5 mM IA.  Matching Kv4 channel properties, activation and inactivation of this IA occur in the subthreshold range of membrane potentials and the rate of recovery from inactivation is rapid and voltage-dependent.  Among Kv4 transcripts, the DRG expresses significant levels of Kv4.1 and Kv4.3 mRNAs.  Also, single small-medium diameter DRG neurons (~30 mm exhibit correlated frequent expression of mRNAs encoding Kv4.1 and Nav1.8, a known nociceptor marker.  In contrast, the expressions of Kv1.4 and Kv4.2 mRNAs at the whole-tissue and single-cell levels are relatively low and infrequent.  Kv4 protein expression in nociceptive DRG neurons was confirmed by immunohistochemistry, which demonstrates colocalization of Kv4.3 and Nav1.8, and negligible expression of Kv4.2.  Furthermore, specific dominant-negative suppression and overexpression strategies confirmed the contribution of Kv4 channels to IA in DRG neurons.  Contrasting the expression patterns of Kv4 channels in the central and peripheral nervous systems, we discuss possible functional roles of these channels in primary sensory neurons.

  14. Application of commercial MOSFET detectors for in vivo dosimetry in the therapeutic x-ray range from 80 kV to 250 kV

    International Nuclear Information System (INIS)

    Ehringfeld, Christian; Schmid, Susanne; Poljanc, Karin; Kirisits, Christian; Aiginger, Hannes; Georg, Dietmar

    2005-01-01

    The purpose of this study was to investigate the dosimetric characteristics (energy dependence, linearity, fading, reproducibility, etc) of MOSFET detectors for in vivo dosimetry in the kV x-ray range. The experience of MOSFET in vivo dosimetry in a pre-clinical study using the Alderson phantom and in clinical practice is also reported. All measurements were performed with a Gulmay D3300 kV unit and TN-502RDI MOSFET detectors. For the determination of correction factors different solid phantoms and a calibrated Farmer-type chamber were used. The MOSFET signal was linear with applied dose in the range from 0.2 to 2 Gy for all energies. Due to fading it is recommended to read the MOSFET signal during the first 15 min after irradiation. For long time intervals between irradiation and readout the fading can vary largely with the detector. The temperature dependence of the detector signal was small (0.3% deg. C -1 ) in the temperature range between 22 and 40 deg. C. The variation of the measuring signal with beam incidence amounts to ±5% and should be considered in clinical applications. Finally, for entrance dose measurements energy-dependent calibration factors, correction factors for field size and irradiated cable length were applied. The overall accuracy, for all measurements, was dominated by reproducibility as a function of applied dose. During the pre-clinical in vivo study, the agreement between MOSFET and TLD measurements was well within 3%. The results of MOSFET measurements, to determine the dosimetric characteristics as well as clinical applications, showed that MOSFET detectors are suitable for in vivo dosimetry in the kV range. However, some energy-dependent dosimetry effects need to be considered and corrected for. Due to reproducibility effects at low dose levels accurate in vivo measurements are only possible if the applied dose is equal to or larger than 2 Gy

  15. Application of commercial MOSFET detectors for in vivo dosimetry in the therapeutic x-ray range from 80 kV to 250 kV.

    Science.gov (United States)

    Ehringfeld, Christian; Schmid, Susanne; Poljanc, Karin; Kirisits, Christian; Aiginger, Hannes; Georg, Dietmar

    2005-01-21

    The purpose of this study was to investigate the dosimetric characteristics (energy dependence, linearity, fading, reproducibility, etc) of MOSFET detectors for in vivo dosimetry in the kV x-ray range. The experience of MOSFET in vivo dosimetry in a pre-clinical study using the Alderson phantom and in clinical practice is also reported. All measurements were performed with a Gulmay D3300 kV unit and TN-502RDI MOSFET detectors. For the determination of correction factors different solid phantoms and a calibrated Farmer-type chamber were used. The MOSFET signal was linear with applied dose in the range from 0.2 to 2 Gy for all energies. Due to fading it is recommended to read the MOSFET signal during the first 15 min after irradiation. For long time intervals between irradiation and readout the fading can vary largely with the detector. The temperature dependence of the detector signal was small (0.3% degrees C(-1)) in the temperature range between 22 and 40 degrees C. The variation of the measuring signal with beam incidence amounts to +/-5% and should be considered in clinical applications. Finally, for entrance dose measurements energy-dependent calibration factors, correction factors for field size and irradiated cable length were applied. The overall accuracy, for all measurements, was dominated by reproducibility as a function of applied dose. During the pre-clinical in vivo study, the agreement between MOSFET and TLD measurements was well within 3%. The results of MOSFET measurements, to determine the dosimetric characteristics as well as clinical applications, showed that MOSFET detectors are suitable for in vivo dosimetry in the kV range. However, some energy-dependent dosimetry effects need to be considered and corrected for. Due to reproducibility effects at low dose levels accurate in vivo measurements are only possible if the applied dose is equal to or larger than 2 Gy.

  16. A continuous acceleration tube of ions under 200 KV; Un tube d'acceleration continue d'ions sous 200 KV

    Energy Technology Data Exchange (ETDEWEB)

    Mongodin, G [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1954-07-01

    The realization of an Van de Graaff accelerator required, for the preliminary studies, the construction of a small proton accelerator, functioning at 200 kV in order to resolve some parasitic effects inherent to the accelerators tubes. The aim of this report is to describe the different organs of the accelerator tube, to explain the operating system and to encode their characteristics. The report first presents the ion source and the beam buncher permitting to inject in the accelerator tube particles of about 9 kV and very batched in a thin beam of circular section. Then the study explain the tube characteristics considered like optic system. A method to obtain precise calculation of particle trajectories is exposed. Aberrations of the system were discussed and balance of the currents on all electrodes inside the tube for different regimes of working were provided. The influence of the residual pressure in the tube were explained. The report finally ends on a part of the fundamental problem of the straining occurring inside the tubes accelerators under high tension. (M.B.) [French] La realisation d n accelerateur du type Van de Graaff a necessite, entre autres etudes preliminaires, la construction d'un petit accelerateur de protons, fonctionnant sous 200 kV afin d'eclaircir certains effets parasites propres aux tubes accelerateurs. L'objet de ce rapport est de decrire les differents organes du tube accelerateur, d'en expliquer le fonctionnement et de chiffrer leurs caracteristiques. Le memoire presente d'abord la source d'ions et le canon permettant d'injecter dans le tube accelerateur des particules de 9 kV environ et bien groupees dans un faisceau fin de section circulaire. Puis il passe a l'etude du tube considere comme systeme optique. Une methode utilisee pour le calcul precis des trajectoires des particules y est exposee. Il aborde le probleme des aberrations de ce systeme et fournit par la suite le bilan des courants sur toutes les electrodes a l

  17. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-09, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    , eller anden årsag. Undervejs omtales en række vigtige detaljer ved spørgeskemaet, men dette notat har ikke til formål at give en udtømmende beskrivelse af hvilke spørgsmål, der er stillet til hvem og hvornår. Her kan det være til stor nytte at besøge testspørgeskemaet på http://tu2010.dk/dev/starttest.php...

  18. The Effect of Phase-to-earth Faults on the Operating Conditions of a Separated 110 kV Grid Normally Operated with Effectively Earthed Neutral, and Temporarily Supplied from a Compensated 110 kV Grid

    Directory of Open Access Journals (Sweden)

    Wilhelm Rojewski

    2015-06-01

    Full Text Available The paper discusses the interoperability of the German compensated 110 kV grid and the Polish effectively earthed 110 kV grid. It is assumed that an area of one grid, separated from its power system, will be temporarily supplied from the other grid in its normal regime. Reference is made to the risks associated with phase-to-earth faults in grids so interconnected. Particular attention is paid to the working conditions of surge arresters and voltage transformers in the Polish 110 kV grid deprived of its neutral earthing when supplied from the German grid.

  19. MicroRNA-Mediated Downregulation of the Potassium Channel Kv4.2 Contributes to Seizure Onset

    Directory of Open Access Journals (Sweden)

    Christina Gross

    2016-09-01

    Full Text Available Seizures are bursts of excessive synchronized neuronal activity, suggesting that mechanisms controlling brain excitability are compromised. The voltage-gated potassium channel Kv4.2, a major mediator of hyperpolarizing A-type currents in the brain, is a crucial regulator of neuronal excitability. Kv4.2 expression levels are reduced following seizures and in epilepsy, but the underlying mechanisms remain unclear. Here, we report that Kv4.2 mRNA is recruited to the RNA-induced silencing complex shortly after status epilepticus in mice and after kainic acid treatment of hippocampal neurons, coincident with reduction of Kv4.2 protein. We show that the microRNA miR-324-5p inhibits Kv4.2 protein expression and that antagonizing miR-324-5p is neuroprotective and seizure suppressive. MiR-324-5p inhibition also blocks kainic-acid-induced reduction of Kv4.2 protein in vitro and in vivo and delays kainic-acid-induced seizure onset in wild-type but not in Kcnd2 knockout mice. These results reveal an important role for miR-324-5p-mediated silencing of Kv4.2 in seizure onset.

  20. Probabilitas Tegangan Sentuh Dan Tegangan Langkah Di Lokasi Rencana Gardu Induk 500 kV Antosari

    Directory of Open Access Journals (Sweden)

    Abdul Latif

    2016-06-01

    Full Text Available Semakin berkembangnya pertindustrian di Indonesia, maka kebutuhan daya listrik yang dibutuhkan semakin meningkat. Untuk memenuhi kebutuhan daya listrik tersebut pada tahun 2016, PT PLN (Persero merencanakan pembangunan GITET 500 kV Antosari. Pembangunan GITET 500 kV Antosari merupakan tindak lanjut dari rencana PT PLN (Persero yang akan menambah pasokan energi listrik ke Bali melalui sistem interkoneksi Jawa – Bali menggunakan jaringan transmisi SUTET 500 kV, dimulai dari GITET 500 kV Paiton dan akan sampai di GITET 500 kV Antosari. Untuk mengamankan gardu induk dari ancaman sambaran petir, salah satu cara yang digunakan adalah dengan mengamankan sistem perntanahan dilokasi gardu induk. Maka dipilih sistem pentanahan grid di lokasi rencana pembangunan Gardu Induk 500 kV Antosari. Penelitian dilakukan untuk menganalisis perbandingan ukuran luas pentanahan dengan kedalaman batang konduktor terhadap tahanan pentanahan grid, tegangan sentuh, tegangan langkah dan probabilitas timbulnya tegangan sentuh dan tegangan langkah. Data tahanan tanah yang didapatkan dari pengukuran secara langsung digunakan untuk mengetahui  nilai tahanan jenis tanah kemudian digunakan untuk menghitung tahanan pentanahan grid, tegangan sentuh, tegangan langkah dan probabilitas tegangan sentuh dan tegangan langkah. Perhitungan tahanan pentanahan grid menggunakan persamaan IEEE, Standard 80-2000 sedangkan untuk perhitungan tegangan sentuh dan tegangan langkah menggunakan IEEE, Standard 665-1995. Berdasarkan hasil penelitian di lokasi gardu induk untuk kondisi tanah basah dengan luas grid 3 m x 3 m dan kedalaman 5 m didapatkan nilai tahanan pentanahan grid 0,49 ohm dan nilai tegangan langkah 125 volt dengan probabilitas 0,72%. Sedangkan untuk kondisi tanah kering dengan luas grid 3 m x 3m dan kedalaman 5 didapatkan nilai tahanan pentanahan grid 1,11 ohm dan nilai tegangan langkah 281 volt dengan probabilitas 0,72%. Dari hasil analisis juga menunjukan dengan luas grid 3 m x 3

  1. Expression and function of Kv1.1 potassium channels in human atria from patients with atrial fibrillation.

    Science.gov (United States)

    Glasscock, Edward; Voigt, Niels; McCauley, Mark D; Sun, Qiang; Li, Na; Chiang, David Y; Zhou, Xiao-Bo; Molina, Cristina E; Thomas, Dierk; Schmidt, Constanze; Skapura, Darlene G; Noebels, Jeffrey L; Dobrev, Dobromir; Wehrens, Xander H T

    2015-09-01

    Voltage-gated Kv1.1 channels encoded by the Kcna1 gene are traditionally regarded as being neural-specific with no known expression or intrinsic functional role in the heart. However, recent studies in mice reveal low-level Kv1.1 expression in heart and cardiac abnormalities associated with Kv1.1-deficiency suggesting that the channel may have a previously unrecognized cardiac role. Therefore, this study tests the hypothesis that Kv1.1 channels are associated with arrhythmogenesis and contribute to intrinsic cardiac function. In intra-atrial burst pacing experiments, Kcna1-null mice exhibited increased susceptibility to atrial fibrillation (AF). The atria of Kcna1-null mice showed minimal Kv1 family ion channel remodeling and fibrosis as measured by qRT-PCR and Masson's trichrome histology, respectively. Using RT-PCR, immunocytochemistry, and immunoblotting, KCNA1 mRNA and protein were detected in isolated mouse cardiomyocytes and human atria for the first time. Patients with chronic AF (cAF) showed no changes in KCNA1 mRNA levels relative to controls; however, they exhibited increases in atrial Kv1.1 protein levels, not seen in paroxysmal AF patients. Patch-clamp recordings of isolated human atrial myocytes revealed significant dendrotoxin-K (DTX-K)-sensitive outward current components that were significantly increased in cAF patients, reflecting a contribution by Kv1.1 channels. The concomitant increases in Kv1.1 protein and DTX-K-sensitive currents in atria of cAF patients suggest that the channel contributes to the pathological mechanisms of persistent AF. These findings provide evidence of an intrinsic cardiac role of Kv1.1 channels and indicate that they may contribute to atrial repolarization and AF susceptibility.

  2. Heteromeric Kv7.2/7.3 channels differentially regulate action potential initiation and conduction in neocortical myelinated axons.

    Science.gov (United States)

    Battefeld, Arne; Tran, Baouyen T; Gavrilis, Jason; Cooper, Edward C; Kole, Maarten H P

    2014-03-05

    Rapid energy-efficient signaling along vertebrate axons is achieved through intricate subcellular arrangements of voltage-gated ion channels and myelination. One recently appreciated example is the tight colocalization of K(v)7 potassium channels and voltage-gated sodium (Na(v)) channels in the axonal initial segment and nodes of Ranvier. The local biophysical properties of these K(v)7 channels and the functional impact of colocalization with Na(v) channels remain poorly understood. Here, we quantitatively examined K(v)7 channels in myelinated axons of rat neocortical pyramidal neurons using high-resolution confocal imaging and patch-clamp recording. K(v)7.2 and 7.3 immunoreactivity steeply increased within the distal two-thirds of the axon initial segment and was mirrored by the conductance density estimates, which increased from ~12 (proximal) to 150 pS μm(-2) (distal). The axonal initial segment and nodal M-currents were similar in voltage dependence and kinetics, carried by K(v)7.2/7.3 heterotetramers, 4% activated at the resting membrane potential and rapidly activated with single-exponential time constants (~15 ms at 28 mV). Experiments and computational modeling showed that while somatodendritic K(v)7 channels are strongly activated by the backpropagating action potential to attenuate the afterdepolarization and repetitive firing, axonal K(v)7 channels are minimally recruited by the forward-propagating action potential. Instead, in nodal domains K(v)7.2/7.3 channels were found to increase Na(v) channel availability and action potential amplitude by stabilizing the resting membrane potential. Thus, K(v)7 clustering near axonal Na(v) channels serves specific and context-dependent roles, both restraining initiation and enhancing conduction of the action potential.

  3. A Common Structural Component for β-Subunit Mediated Modulation of Slow Inactivation in Different KV Channels

    DEFF Research Database (Denmark)

    Strutz-Seebohm, Nathalie; Henrion, Ulrike; Schmitt, Nicole

    2013-01-01

    Background/Aims: Potassium channels are tetrameric proteins providing potassium selective passage through lipid embedded proteinaceous pores with highest fidelity. The selectivity results from binding to discrete potassium binding sites and stabilization of a hydrated potassium ion in a central...... internal cavity. The four potassium binding sites, generated by the conserved TTxGYGD signature sequence are formed by the backbone carbonyls of the amino acids TXGYG. Residues KV1.5-Val481, KV4.3-Leu368 and KV7.1- Ile 313 represent the amino acids in the X position of the respective channels. Methods...

  4. Comparative randomized study on the efficaciousness of treatment of BOO due to BPH in patients with prostate up to 100 gr by endoscopic gyrus prostate resection versus open prostatectomy. Preliminary data.

    Science.gov (United States)

    Giulianelli, Roberto; Brunori, Stefano; Gentile, Barbara Cristina; Vincenti, Giorgio; Nardoni, Stefano; Pisanti, Francesco; Shestani, Teuta; Mavilla, Luca; Albanesi, Luca; Attisani, Francesco; Mirabile, Gabriella; Schettini, Manlio

    2011-06-01

    With the advent of medical management and minimally techniques for benign prostate hypeplasia (BPH), invasive surgical procedures such open prostatectomy (OPSU) have become less common, although selected patients may still benefit from open prostatectomy. Aim of this study was to evaluate efficacy and safety of Bipolar TURP (Gyrus electro surgical system) versus standard open prostatectomy in patients with lower urinary tract symptoms (LUTS) due to bladder outlet obstruction (BOO) with markedly enlarged glands refractory to medical therapy. From January 2003 to January 2004, 140 patients affected by mild-severe LUTS, secondary to BOO from BPH, refractory to medical therapy, with markedly enlarged glands, were randomized in two groups (1:1), and subjected to open prostatectomy (OPSU) carried out with traditional method (Bracci Thechnique) versus transurethral resection of the prostate (TURP) utilizing the bipolar methodology. Preoperative work-up included IPSS, IIEF-5 and Qol questionnaires. All patients were submitted to uroflowmetry, transrectal ultrasound (TRUS), measurament of postvoidal residual urine and PSA determination. IPSS, IIEF-5 and Qol, uroflowmetry, TRUS, measurement of post-voidal residual urine, PSA determination and number of reoperations were evaluated at 1, 3, 6, 12, 18, 24, 30 and 36 months. Operative time, resected tissue weight and perioperative complications were also registered. Total post-operative catheter time, total postoperative hospital stay, haemoglobin loss were recorded in the 2 groups. Comparative data on IPSS symptom score, IIEF-5 and Qol, PSA, peak urinary flow rates and post-void residual urine volume in the 2 groups were similar but showed a significative improvement with respect to baseline value. Postoperative haemoglobin levels, postoperative catheterization, hospital stay and 3-yr overall surgical re-treatment-free rate were significantly better in the Bipolar group. In the treatment of LUTS due to bladder outlet

  5. Preventive Effect of TU-100 on a Type-2 Model of Colitis in Mice: Possible Involvement of Enhancing Adrenomedullin in Intestinal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Atsushi Kaneko

    2013-01-01

    Full Text Available Purpose. Crohn's disease (CD and ulcerative colitis (UC, the two major forms of inflammatory bowel disease (IBD, have histopathologically and immunologically different characteristics. We previously reported that a traditional Japanese medicine, daikenchuto (TU-100, ameliorated a trinitrobenzenesulfonic acid- (TNBS- induced type-1 model colitis exhibiting histopathological features of CD through adrenomedullin (ADM enhancement. Our current aims were to examine whether TU-100 ameliorates a type-2 model colitis that histologically resembles UC and identify the active ingredients. Methods. TU-100 was administered orally to mice with oxazolone- (OXN- induced type-2 model colitis. The morbidity was evaluated by body weight loss and the macroscopic score of colonic lesions. ADM was quantified using an EIA kit. Results. TU-100 prevented weight loss and colon ulceration. ADM production by intestinal epithelial cells was increased by TU-100 addition. Screening to identify active ingredients showed that [6]-shogaol and hydroxy α-sanshool enhanced ADM production. Conclusions. TU-100 exerted a protective effect in OXN-induced type-2 model colitis, indicating that TU-100 may be a beneficial agent for treatment of UC.

  6. Full Scale Test on a 100km, 150kV AC Cable

    DEFF Research Database (Denmark)

    Faria da Silva, Filipe Farria; Wiechowski, W.; Bak, Claus Leth

    2010-01-01

    phenomena is conducted. The cases analysed in this paper are: Zero-missing phenomenon, Ferranti effect, energisation transient, effect of the cable's connection in the busbar voltage and cable disconnection. For all the phenomena described in the paper measurement data are presented and it is verified......This paper presents some of the results obtained from the electrical measurements on a 99.7 km, 150 kV three-phase AC cable, connecting 215 MW offshore wind farm Horns Rev 2, located in Denmark west coast, to Denmark's 400 kV transmission network. The measurements were performed at nominal voltage...... if the obtained results are in accordance with the theory and also with simulations performed in PSCAD/EMTDC. With the exception of the cable disconnection, for all the remaining cases introduced in this paper the measurements confirmed the theoretical expectations. Depending on the cable disconnection sequence...

  7. Portable and wireless IV-curve tracer for >5 kV organic photovoltaic modules

    DEFF Research Database (Denmark)

    Garcia Valverde, Rafael; Chaouki-Almagro, Samir; Corazza, Michael

    2016-01-01

    voltage applications, the design is based on low cost components, battery-based isolated supply and wireless communication. A prototype has been implemented and field tested for characterization of different organic photovoltaic modules (OPV) made according to the infinity concept with a large number......The practical design of a wirelessly controlled portable IV-curve tracer based on a capacitive load is described. The design is optimized for the measurement of solar cell modules presenting a high open circuit voltage of up to 6 kV and a low short circuit current below 100 mA. The portable IV......-tracer allows for on-site/in-situ characterization of large modules under real operating conditions and enables fast detection of potential failure of anomalies in electrical behavior. Currently available electronic loads only handle voltages up to around 1 kV. To overcome cost and safety issues related to high...

  8. Design for a FET based 1 MHz, 10 kV pulse generator

    International Nuclear Information System (INIS)

    Barnes, M.J.; Wait, G.D.

    1995-08-01

    A pulse generator consisting of a coaxial cable and a high voltage modulator, incorporating two stacks of Field-Effect Transistor (FET) switches operating in ''push-pull'' mode, has been designed and built. The modulator generates a continuous, unipolar, pulse train at a fundamental frequency of 1 MHz and a magnitude of 10 kV. The rise and fall times of the pulses are less than 39 ns. The two stacks each utilize 14 FETS, which are individually rated at 1 kV. The design incorporates a low-loss coaxial cable on which pulses are stored. Extensive PSpice simulations have been carried out to evaluate various design options. Subsequent measurements on the prototype pulse generator confirm the PSpice predictions. This system is applicable for the kicker system at TRIUMF

  9. Internal services simulation control in 220/110kV power transformer station Mintia

    Science.gov (United States)

    Ciulica, D.; Rob, R.

    2018-01-01

    The main objectives in developing the electric transport and distribution networks infrastructure are satisfying the electric energy demand, ensuring the continuity of supply to customers, minimizing electricity losses in the transmission and distribution networks of public interest. This paper presents simulations in functioning of the internal services system 400/230 V ac in the 220/110 kV power transformer station Mintia. Using simulations in Visual Basic, the following premises are taken into consideration. All the ac consumers of the 220/110 kV power transformer station Mintia will be supplied by three 400/230 V transformers for internal services which can mutual reserve. In case of damaging at one transformer, the others are able to assume the entire consumption using automatic release of reserves. The simulation program studies three variants in which the continuity of supply to customers are ensured. As well, by simulations, all the functioning situations are analyzed in detail.

  10. JAERI 200 kV electron gun with an NEA-GaAs photocathode

    International Nuclear Information System (INIS)

    Nishitani, Tomohiro; Minehara, Eisuke J.; Hajima, Ryoichi; Nagai, Ryoji; Sawamura, Masaru; Nishimori, Nobuyuki; Kikuzawa, Nobuhiro; Yamauchi, Toshihiko

    2004-01-01

    The photocathode DC-gun with high average current, low beam emittance and long operational lifetime is considered to be indispensable for ERL-FEL. We have started the development program of a 200 keV electron gun with the NEA-GaAs photocathode for the first time in JAERI. In order to long an NEA surface lifetime, the JAERI 200 kV electron gun system consists of a 200 kV DC-gun chamber on extreme high vacuum condition and an NEA activation chamber with load-lock system. We report the goal of photocathode DC-gun R and D and the schedule of a developmental program. (author)

  11. 80 kV electrostatic wire septum for AmPS

    International Nuclear Information System (INIS)

    Linden, A. van der; Bijleveld, J.H.M.; Rookhuizen, H.B.; Bruinsma, P.J.T.; Heine, E.; Lassing, P.; Prins, E.

    1992-01-01

    The Amsterdam Pulse Stretcher (AmPS) ring aims at a 100% duty cycle operation by means of slow extraction of injected electron beam pulses of 2.1 μs. In the extraction process of the AmPS, the extracted beam is intercepted from the circulating beam by the 1 m long electrostatic wire septum. For a bending angle of 4.4 mrad the maximum anode voltage is 80 kV. Care has been given to the electric field distribution at the entrance and exit of the septum and to the insulators, required to support the anode. Prototype tests have been successful up to an anode voltage of 120 kV. (R.P.) 9 refs.; 5 figs

  12. Prevalence of resistance to antibiotics according to International Classification of Diseases (ICD-10) in Boo Ali Sina Hospital of Sari, 2011-2012.

    Science.gov (United States)

    Afshar, Parvaneh; Saravi, Benyamin Mohseni; Nehmati, Ebrahim; Farahabbadi, Ebrahim Bagherian; Yazdanian, Azadeh; Siamian, Hasan; Vahedi, Mohammad

    2013-01-01

    One of the issues in health care delivery system is resistance to antibiotics. Many researches were done to show the causes and antibiotics which was resistance. In most researches the methods of classifying and reporting this resistance were made by researcher, so in this research we examined the International Classification of Diseases 10 the edition (ICD-10). This is a descriptive cross section study; data was collected from laboratory of Boo Ali Sina hospital, during 2011-2012. The check list was designed according the aim of study. Variables were age, bacterial agent, specimen, and antibiotics. The bacteria and resistance were classified with ICD-10. The data were analyzed with SPSS (16) soft ware and the descriptive statistics. Results showed that of the 10198 request for culture and antibiogram, there were 1020(10%) resistance. The specimen were 648 (63.5%) urine, blood 127(12.5%), other secretion 125 (12/3%), sputum 102 (10%), lumbar puncture 8 (0/8%), stool 6 (6/0%) and bone marrow 4 (0.4%). The E coli was the most 413 (40.5%) resistance cause to antibiotics which was coded with B96.2 and the most resistance was to multiple antibiotics 885(86.8%) with the U88 code. The results showed that by using the ICD-10 codes, the study of multiple causes and resistance is possible. The routine usage of coding of the ICD-10 would result to an up to date bank of resistance to antibiotics in every hospitals and useful for physicians, other health care, and health administrations.

  13. An improved method for determining the purity of jet fuels in a POZ-TU instrument

    Energy Technology Data Exchange (ETDEWEB)

    Zrelov, V N; Fedotkin, B I; Krasnaya, L V; Nikitin, L V; Postinkova, N G

    1983-01-01

    The possibility is studied of real time testing for the content of mechanical impurities (Cm.t.) in jet fuels (RT) in a POZ-TU instrument. Based on the obtained data, a four point scale of gray standards is developed for determining the mechanical impurity content, which is four rounded, gray stamps of different intensity, which corresponds to a mechanical impurity content of 0.5; 1.0; 2.0 and 3.0 milligrams per liter. A white indicator filtering element is built into the POZ-TU for determining the mechanical impurity content, and 50 cubic centimeters of the jet fuel are pumped through it over the course of several seconds. The mechanical impurities are placed on the indicator element, forming an imprint, the intensity of the color of which corresponds to the content of mechanical impurities in the jet fuel. The indicator element is extracted from the instrument and the prints are compared with the scale of gray standards, from which the content of the mechanical impurities is determined.

  14. The "Da Ming Hunyi Tu": Repurposing a Ming Map in Sino-African Diplomacy

    Directory of Open Access Journals (Sweden)

    Alexander Akin

    2016-12-01

    Full Text Available In 2002, an exhibition at South Africa’s parliament included a reproduction of the Da Ming Hunyi Tu (Amalgamated map of the Great Ming, citing it as the earliest world map to depict the entire African continent. As part of its broader efforts to shape a narrative of long-standing and peaceful international relations with Africa, the People’s Republic of China formally presented a replica of this map as a gift to the South African government in conjunction with the exhibition. In official statements and popular media coverage alike, the map was described as evidence of a distinctly Chinese approach to global relations, based on benevolence and mutual respect. In particular, the map was ahistorically intertwined with the legacy of Zheng He’s diplomatic expeditions, which reached the East African coast in the early 1400s. To the cartographic historian, however, the depiction of Africa in the Da Ming Hunyi Tu is clearly derived from non-Chinese sources that predate Zheng He’s expeditions. This article examines the ways in which the map has been divorced from its original context to suit modern needs, exemplifying the deployment of cartography to deflect anxieties about the nature of Chinese economic influence in South Africa.

  15. TuBaFrost 5: multifunctional central database application for a European tumor bank.

    Science.gov (United States)

    Isabelle, M; Teodorovic, I; Morente, M M; Jaminé, D; Passioukov, A; Lejeune, S; Therasse, P; Dinjens, W N M; Oosterhuis, J W; Lam, K H; Oomen, M H A; Spatz, A; Ratcliffe, C; Knox, K; Mager, R; Kerr, D; Pezzella, F; van de Vijver, M; van Boven, H; Alonso, S; Kerjaschki, D; Pammer, J; Lopez-Guerrero, J A; Llombart Bosch, A; Carbone, A; Gloghini, A; van Veen, E-B; van Damme, B; Riegman, P H J

    2006-12-01

    Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect TuBaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the TuBaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre. The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails.

  16. An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells

    Energy Technology Data Exchange (ETDEWEB)

    Whitney, John C.; Quentin, Dennis; Sawai, Shin; LeRoux, Michele; Harding, Brittany N.; Ledvina, Hannah E.; Tran, Bao Q.; Robinson, Howard; Goo, Young Ah; Goodlett, David R.; Raunser, Stefan; Mougous, Joseph D.

    2015-10-08

    Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD+ and NADP+. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.

  17. The infrared flux method and its use for study of α Boo, μ Her and β Dra; relation to the Vega 1.2-5 μm infrared excess

    International Nuclear Information System (INIS)

    Blackwell, D.E.; Booth, A.J.; Petford, A.D.; Leggett, S.K.; Mountain, C.M.; Selby, M.J.

    1986-01-01

    The effective temperatures of α Boo, μ Her, and β Dra are determined using the infrared flux method. The determinations are based on the high-quality spectrophotometric data obtained from an aircraft by previous authors, the new infrared calibration of Vega obtained from observations made at Tenerife between 1979 and 1983, the use of a Reticon spectrometer to determine the integrated fluxes in the region 370-950 nm, and the use of model atmospheres constructed with the previously developed MARCS code. (author)

  18. Design and development of a high-power, 500 kV pulsed line

    International Nuclear Information System (INIS)

    Nicolas, A.

    A study was made of very high voltage (500 kV) pulse production for 50 ns at half height. A coaxial line was coupled to a Marx generator for obtaining the pulses on an impedance-adapted electron diode. The maximum power obtained was 6.4 x 10 10 W with a current front in the diode of about 30 ns (80 ns pulse length at half height)

  19. Method of selecting optimum cross arm lengths for a 750 kV transmission line

    Energy Technology Data Exchange (ETDEWEB)

    Aleksandrov, G N; Olorokov, V P

    1965-01-01

    A method is presented, based on both technical and economic considerations, for selecting cross arm lengths for intermediate poles of power transmission lines according to the effects of internal overvoltage, methods from probability theory and mathematical statistics employed. The problem of optimum pole size is considered in terms of the effect of internal overvoltages for a prescribed maximum level of 2.1 PU currently used in the USSR for the design of 750 kV lines.

  20. Preliminary design of a 100 Hz, 350 kV short pulse generator

    International Nuclear Information System (INIS)

    Rohwein, G.J.; Buttram, M.T.

    1977-06-01

    This report describes a 350 kV pulser designed to generate 100 ns square pulses with 300 joules total energy at a pulse repetition frequency of 100 per second. This design incorporates a transformer charged helical coaxial pulse forming line. The considerations leading to this design are presented together with results from prototype experiments. The pulser which is presently in the construction and testing phase is described in detail. The pulser will be used for electron beam acceleration

  1. STUDY ON PERFORMANCE OF 21M 132kV TRANSMISSION TOWER WITH MEDIUM WIND INTENSITY

    OpenAIRE

    V. LAKSHMI; A. RAJAGOPALA RAO

    2012-01-01

    Electric Power is today playing an increasingly important role in the life of the community. In the electric power system the production and transmission of power are two predominant factors. For the purpose of transmission of electricity towers are the main medium with some wires at required distances and altitudes. The remotehydroelectric power plants have given rise to the need for extra high voltage. Prior to 1950, 150 kV electric transmission lines were considered and still higher voltag...

  2. Regulation System for the 18 kV/90 Mvar Compensators

    CERN Document Server

    Burdet, G

    2001-01-01

    Two 18 kV/90 Mvar static compensators are involved to stabilise the voltage, filter the harmonics and compensate the reactive power generated by the power converters used to supply the SPS accelerator magnets. The in-house hardware and software used by the regulation systems, difficult to maintain and upgrade, shall be renovated. Industrial solution based on PLC will be implemented. This paper describes the future system and its integration to the Electrical Network Supervisor.

  3. Optimizing monoscopic kV fluoro acquisition for prostate intrafraction motion evaluation

    International Nuclear Information System (INIS)

    Adamson, Justus; Wu Qiuwen

    2009-01-01

    Monoscopic kV imaging during radiotherapy has been recently implemented for prostate intrafraction motion evaluation. However, the accuracy of 3D localization techniques from monoscopic imaging of prostate and the effect of acquisition parameters on the 3D accuracy have not been studied in detail, with imaging dose remaining a concern. In this paper, we investigate methods to optimize the kV acquisition parameters and imaging protocol to achieve improved 3D localization and 2D image registration accuracy for minimal imaging dose. Prostate motion during radiotherapy was simulated using existing cine-MRI measurements, and was used to investigate the accuracy of various 3D localization techniques and the effect of the kV acquisition protocol. We also investigated the relationship between mAs and the accuracy of the 2D image registration for localization of fiducial markers and we measured imaging dose for a 30 cm diameter phantom to evaluate the necessary dose to achieve acceptable image registration accuracy. Simulations showed that the error in assuming the shortest path to localize the prostate in 3D using monoscopic imaging during a typical IMRT fraction will be less than ∼1.5 mm for 95% of localizations, and will also depend on prostate motion distribution, treatment duration and image acquisition and treatment protocol. Most uncertainty cannot be reduced from higher imaging frequency or acquiring during gantry rotation between beams. Measured maximum surface dose to the cylindrical phantom from monoscopic kV intrafraction acquisitions varied between 0.4 and 5.5 mGy, depending on the acquisition protocol, and was lower than the required dose for CBCT (21.1 mGy). Imaging dose can be lowered by ∼15-40% when mAs is optimized with acquisition angle. Images acquired during MV beam delivery require increased mAs to obtain the same level of registration accuracy, with mAs/registration increasing roughly linearly with field size and dose rate.

  4. Protection system for 11kV network using arc noise

    International Nuclear Information System (INIS)

    Burdi, M.K.; Memon, A.S.; Shaikh, S.A.

    2000-01-01

    Extensive research work is being done on protecting devices for 11KV network using arc noise and fault noise frequencies in relay to operate a circuit breaker; The relay works on the principle of detecting fault and extracting voltage signal output from the noise frequencies across filter. The voltage development without amplification, is enough to operate the circuit breaker. Sensitivity of the circuit breaker. In this paper design, operation and application of the relay are described. (author)

  5. AC transmission, with very high voltages and the 750 kV line

    Energy Technology Data Exchange (ETDEWEB)

    Bocker, H

    1964-01-01

    The economic case for adoption of extra-high voltages for transmitting electric power over distances of the order of 1000 km is discussed. Some special technical developments for solving the problems attached to such high voltages are briefly discussed, particularly in the fields of switching and transients suppression. The first 750-kV projects in Canada and Russia are mentioned. Equipment, e.g., bushings, transformers, etc., operating at such voltages are illustrated.

  6. Diagnostics system for the 67 MJ, 50 kV pulsed power capacitor bank

    Energy Technology Data Exchange (ETDEWEB)

    Galakhov, I V; Gasheev, A S; Gruzin, I A; Gudov, S N; Murugov, V M; Osin, V A; Pankratov, V I; Pegoev, I N [All-Russian Scientific Research Inst. of Experimental Physics, Sarov (Russian Federation)

    1997-12-31

    The diagnostics system is designed for charging and discharging to the load of the large 67 MJ and 50 kV capacitor bank for the iodine laser pulse power of ISKRA-5 facility. Discharging diagnostics of the capacitor bank uses a technique to measure a sequence of times between representative discharge events for 665 discharge circuits of the bank. Benefits of the measurement techniques are discussed. (author). 3 figs., 3 refs.

  7. Studi Keamanan dan Keandalan Suplai Sistem Kelistrikan Bali Sesuai Rencana Operasi SUTET 500 kV

    Directory of Open Access Journals (Sweden)

    Bawa Adiputra

    2014-12-01

    Full Text Available Kondisi eksisting kelistrikan Bali hingga tahun 2013 disuplai oleh tiga pembangkit tenaga listrik dan sebuah sistem interkoneksi Jamali dengan keseluruhan daya suplai sebesar 676 MW. Berdasarkan peramalan beban sistem Bali 2013-2030 diperoleh rata-rata pertumbuhan beban 6,61%. Berdasarkan hal tersebut PT PLN (Persero merencanakan penambahan pasokan daya listrik ke pulau Bali dengan penambahan kabel laut, suplai melalui SUTET 500 kV interkoneksi Jawa-Bali, serta pembangunan PLTU Celukan Bawang 980 MW. Dengan beroperasinya unit-unit tersebut diperlukan analisis keamanan suplai sistem kelistrikan Bali, dengan dua skenario. Skenario 1 yaitu sistem Bali hanya menerima pasokan dari PLTU Celukan Bawang pada tahun 2016 sebesar 380 MW. Skenario 2, PLTU Celukan Bawang beroperasi sebesar 380 MW tahun 2016, kemudian pada tahun 2020 dan 2022 menambah daya masing-masing 300 MW. Selain analisis keamanan suplai dilakukan juga analisis keandalan dengan beroperasinya SUTET 500 kV di Bali. Analisis keandalan SUTET 500 kV menggunakan tiga skenario. Skenario 1 SUTET beroperasi dari Paiton sampai di GITET Gilimanuk, Skenario 2 SUTET beroperasi dari PAITON sampai di GITET New Kapal, dan Skenario 3 SUTET beroperasi dari Paiton sampai di GITET Kapal. Dari hasil analisis keamanan suplai diperoleh skenario 1, pada tahun 2022 beban puncak mencapai 1304,10 MW dan pada kondisi N-1 mengalami krisis daya listrik -48,3 MW. Skenario 2, pada tahun 2028 dengan beban puncak 1862,60 MW dan pada kondisi N-1 mengalami krisis daya listrik -6,8 MW. Sedangkan analisis keandalan beroperasinya SUTET 500 kV di Bali diperoleh: Skenario 1 nilai SAIFI = 2,0 kali/km/tahun dan nilai SAIDI = ±10 menit/tahun, Skenario 2 nilai SAIFI = 3,1 kali/km/tahun dan nilai SAIDI = ±15 menit/tahun, Skenario 3 nilai SAIFI = 3,3 kali/km/tahun dan nilai SAIDI = ±17 menit/tahun.

  8. Diagnostics system for the 67 MJ, 50 kV pulsed power capacitor bank

    International Nuclear Information System (INIS)

    Galakhov, I.V.; Gasheev, A.S.; Gruzin, I.A.; Gudov, S.N.; Murugov, V.M.; Osin, V.A.; Pankratov, V.I.; Pegoev, I.N.

    1996-01-01

    The diagnostics system is designed for charging and discharging to the load of the large 67 MJ and 50 kV capacitor bank for the iodine laser pulse power of ISKRA-5 facility. Discharging diagnostics of the capacitor bank uses a technique to measure a sequence of times between representative discharge events for 665 discharge circuits of the bank. Benefits of the measurement techniques are discussed. (author). 3 figs., 3 refs

  9. Aberration-corrected STEM/TEM imaging at 15 kV

    International Nuclear Information System (INIS)

    Sasaki, Takeo; Sawada, Hidetaka; Hosokawa, Fumio; Sato, Yuta; Suenaga, Kazu

    2014-01-01

    The performance of aberration-corrected (scanning) transmission electron microscopy (S/TEM) at an accelerating voltage of 15 kV was evaluated in a low-voltage microscope equipped with a cold-field emission gun and a higher-order aberration corrector. Aberrations up to the fifth order were corrected by the aberration measurement and auto-correction system using the diffractogram tableau method in TEM and Ronchigram analysis in STEM. TEM observation of nanometer-sized particles demonstrated that aberrations up to an angle of 50 mrad were compensated. A TEM image of Si[110] exhibited lattice fringes with a spacing of 0.192 nm, and the power spectrum of the image showed spots corresponding to distances of 0.111 nm. An annular dark-field STEM image of Si[110] showed lattice fringes of (111) and (22¯0) planes corresponding to lattice distances of 0.314 nm and 0.192 nm, respectively. At an accelerating voltage of 15 kV, the developed low-voltage microscope achieved atomic-resolution imaging with a small chromatic aberration and a large uniform phase. - Highlights: • Aberration-corrected STEM/TEM imaging at 15 kV demonstrated lattice fringes of Si[110] single crystal with a spacing of 0.192 nm. • To achieve this performance at a lower accelerating voltage, uniform phase area over 50 mrad is mandatory in Ronchigram and Diffractogram tableau. • This means a higher-order aberration of six-fold astigmatism should be compensated. • In addition, decreasing the effect of chromatic aberration plays an important role for improving the performance of linear scattering component at 15 kV TEM

  10. A Study of Electromagnetic Radiation of Corona Discharge Near 500-Kv Electric Installations

    International Nuclear Information System (INIS)

    Korzhov, A. V.; Okrainskaya, I. S.; Sidorov, A. I.; Kufel'd, V. D.

    2004-01-01

    Data on the spectral composition and intensity of electromagnetic radiation of corona discharge are obtained in an experimental study performed on the outdoor switchgear of the Shagol 500-kV substation of the Chelyabinsk Enterprise of Trunk Transmission Grids and under a 500-kV Shagol - Kozyrevo overhead transmission line. The electromagnetic environment on the territory of the 500-kV outdoor switchgear is shown to be determined by narrow-band radiations (harmonics of the frequency of electric supply) and wide-band radiations due to corona discharges of high-voltage sources. This means that the personnel experience the action of a commercial-frequency electric field and electromagnetic radiation of a quite wide range, which is not allowed for by the existing guidelines. It is recommended to continue the study in cooperation with medical institutions in order to create guidelines that would allow for the joint action of commercial-frequency electric field and electromagnetic radiation and for the voltage in the line, the current load, the meteorological situation, and other factors

  11. Clicked bis-PEG-peptide conjugates for studying calmodulin-Kv7.2 channel binding.

    Science.gov (United States)

    Bonache, M Angeles; Alaimo, Alessandro; Malo, Covadonga; Millet, Oscar; Villarroel, Alvaro; González-Muñiz, Rosario

    2014-11-28

    The recombinant Kv7.2 calmodulin (CaM) binding site (Q2AB CaMBD) shows a high tendency to aggregate, thus complicating biochemical and structural studies. To facilitate these studies we have conceived bis-PEG-peptide CaMBD-mimetics linking helices A and B in single, easy to handle molecules. Short PEG chains were selected as spacers between the two peptide molecules, and a Cu(i)-catalyzed cycloaddition (CuAAC) protocol was used to assemble the final bis-PEG-peptide conjugate, by the convenient functionalization of PEG arms with azide and alkyne groups. The resulting conjugates, with a certain helical character in TFE solutions (CD), showed nanomolar affinity in a fluorescence CaM binding in vitro assay, higher than just the sum of the precursor PEG-peptide affinities, thus validating our design. The approach to these first described examples of Kv7.2 CaMBD-mimetics could pave the way to chimeric conjugates merging helices A and B from different Kv7 subunits.

  12. Measurements on a FET based 1 MHz, 10 kV pulse generator

    International Nuclear Information System (INIS)

    Wait, G.D.; Barnes, M.J.

    1995-08-01

    A prototype pulser, which incorporates thirty-two 1 kV Field-Effect Transistor (FET) modules, has been built and tested at TRIUMF. The pulser has been developed for application in a scheme for pulsed extraction from the TRIUMF 500 MeV cyclotron. Deflection of the beam will be provided by an electric field between a set of 1 in long deflector plates. The pulser generates a continuous, unipolar, pulse train at a fundamental frequency of approximately 1 MHz and a magnitude of 10 kV. The pulses have 38 ns rise and fall times and are stored on a low-loss coaxial cable which interconnects the pulse generator and the deflector plates. The circuit performance was evaluated with the aid of PSpice in the design stage and confirmed by measurements on the prototype. Temperature measurements have been performed on 1 kV FET modules under DC conditions and compared with temperatures under operating conditions to ensure that switching losses are acceptable. Results of various measurements are presented and compared with simulations

  13. Measurements on a FET based 1 MHz, 10 kV pulse generator

    International Nuclear Information System (INIS)

    Wait, G.D.; Barnes, M.J.

    1995-08-01

    A prototype pulser, which incorporates thirty-two 1 kV Field-Effect Transistor (FET) modules, has been built and tested at TRIUMF. The pulser has been developed for application in a scheme for pulsed extraction from the TRIUMF 500 MeV cyclotron. Deflection of the beam will be provided by an electric field between a set of 1 m long deflector plates. The pulser generates a continuous unipolar, pulse train at a fundamental frequency of approximately 1 MHz and a magnitude of 10 kV. The pulses have 38 ns rise and fall times and are stored on a low-loss coaxial cable which interconnects the pulse generator and the deflector plates. The circuit performance was evaluated with the aid of PSpice in the design stage and confirmed by measurements on the prototype. Temperature measurements have been performed on 1 kV FET modules under DC conditions and compared with temperatures under operating conditions to ensure that switching losses are acceptable. Results of various measurements are presented and compared with simulations. (author)

  14. KV7 Channels Regulate Firing during Synaptic Integration in GABAergic Striatal Neurons

    Directory of Open Access Journals (Sweden)

    M. Belén Pérez-Ramírez

    2015-01-01

    Full Text Available Striatal projection neurons (SPNs process motor and cognitive information. Their activity is affected by Parkinson’s disease, in which dopamine concentration is decreased and acetylcholine concentration is increased. Acetylcholine activates muscarinic receptors in SPNs. Its main source is the cholinergic interneuron that responds with a briefer latency than SPNs during a cortical command. Therefore, an important question is whether muscarinic G-protein coupled receptors and their signaling cascades are fast enough to intervene during synaptic responses to regulate synaptic integration and firing. One of the most known voltage dependent channels regulated by muscarinic receptors is the KV7/KCNQ channel. It is not known whether these channels regulate the integration of suprathreshold corticostriatal responses. Here, we study the impact of cholinergic muscarinic modulation on the synaptic response of SPNs by regulating KV7 channels. We found that KV7 channels regulate corticostriatal synaptic integration and that this modulation occurs in the dendritic/spines compartment. In contrast, it is negligible in the somatic compartment. This modulation occurs on sub- and suprathreshold responses and lasts during the whole duration of the responses, hundreds of milliseconds, greatly altering SPNs firing properties. This modulation affected the behavior of the striatal microcircuit.

  15. Improvement of the 400 kV linac electron source of AmPS

    International Nuclear Information System (INIS)

    Kroes, F.B.; Beuzekom, M.G. van; Dobbe, N.J.; Es, J.T. van; Jansweijer, P.P.M.; Kruijer, A.H.; Luigjes, G.; Sluijk, T.G.B.

    1992-01-01

    An existing linac (MEA) injects electrons into the Amsterdam Pulse Stretcher (AmPS) ring. The linac's peak current increases from 20 to 80 mA. This requires the modification of the 400 kV low emittance gun. The fourfold increase of the peak current is obtained by doubling both the gun perveance (new gun part) and the pulsed extractor voltage. To obtain optimum beam quality over this increased current range, the hot deck electronics has been exchanged by a fast high voltage FET switching supply. A built-in microprocessor, coupled to the local computer by optical fibers, is used to monitor and control the gun parameters. The 5 kV gun extractor voltage pulse shape can be monitored by means of an analog fibre transducer with build in calibration. Finally, in order to improve the energy stability of the accelerated electrons, a serial electron-tube stabilizer was added to the 400 kV DC power supply. (K.A.) 4 refs.; 6 figs

  16. A 120kV IGBT modulator for driving a Pierce electron gun

    International Nuclear Information System (INIS)

    Earley, Lawrence M.; Brown, Richard W.; Carlson, Randolph L.; Ferguson, Patrick; Haynes, William B.; Kirbie, Hugh C.; Russell, Steven J.; Sigler, Floyd E.; Smirnova, Evgenya I.; Wheat, Robert M. Jr.

    2004-01-01

    An IGBT modulator has been developed to drive a 120 kV, 23 A Pierce electron gun. The modulator is capable of producing pulses up to 10 μs in width at repetition rates up to 10Hz with no active reset. The pulse rise time on the electron gun will be approximately 2 μs and the remaining 8 μs of flattop is tuned to have a ripple of less than 1 percent rms. The modulator technology was developed from a previous 50 kV prototype. The modulator consists of six boards, each with one EUPEC IGBT that drives a single common step-up transformer wound on METGLAS 2605SC cores. The six transformer cores share a common bi-filar output secondary winding. The modulator uses a fiber optic trigger system and has a high voltage cable output with an epoxy receptacle on the oil end and a ceramic receptacle on the vacuum end. The 120 kV electron gun was manufactured by MDS Co. and will be used to generate sheet electron beams from the standard pencil beam produced by the Pierce electron gun.

  17. Implication of novel thiazolo-thiophene derivative (MCD-KV-10) for management of asthma.

    Science.gov (United States)

    Patil, Dhiraj; Dash, Ranjeet Prasad; Thakur, Sandeep Kumar; Pandya, Amit N; Venkatesh, P; Vasu, Kamala K; Nivsarkar, Manish

    2015-04-01

    Asthma is multifaceted disease where many targets contribute towards its development and progression. Among these, adenosine receptor subtypes play a major role. MCD-KV-10, a novel thiazolo-thiophene was designed and evaluated pre-clinically for its implication in management of asthma. This compound showed good affinity and selectivity towards A(2A)/A3 adenosine receptor (AR) subtypes. Furthermore, MCD-KV-10 was evaluated for in vitro lipoxygenase inhibition activity; in vivo mast cell stabilization potential and in vivo anti-asthmatic activity was done in ovalbumin-induced airway inflammation model in guinea pigs. The compound showed good (>57%) inhibition of lipoxygenase enzyme and also effectively protected mast cell degranulation (>63%). The compound showed good anti-asthmatic activity as inferred from the in vivo studies. These results indicate that MCD-KV-10 has an inhibitory effect on airway inflammation. Though, we have identified a potential candidate for management of asthma, further mechanistic studies are needed.

  18. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    NARCIS (Netherlands)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

    2008-01-01

    Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

  19. Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II)

    DEFF Research Database (Denmark)

    Rasmussen, Nils-Jørgen; Wikman, Friedrik; Clark, Brian F. C.

    1990-01-01

    A tRNA containing a long extra arm, namely E. coli tRNA1Leu has been crosslinked to elongation factor Tu, with the crosslinking reagent trans-diamminedichloroplatinum(II). The nucleotide involved in the crosslinking was identified to be a guanosine in the variable region at position 47F or 47G....

  20. Mitochondrial translation factors of Trypanosoma brucei: elongation factor-Tu has a unique subdomain that is essential for its function

    Czech Academy of Sciences Publication Activity Database

    Cristodero, M.; Mani, J.; Oeljeklaus, S.; Aeberhard, L.; Hashimi, Hassan; Ramrath, D.J.F.; Lukeš, Julius; Warscheid, B.; Schneider, A.

    2013-01-01

    Roč. 90, č. 4 (2013), s. 744-755 ISSN 0950-382X R&D Projects: GA ČR GAP305/12/2261 Institutional support: RVO:60077344 Keywords : mitochondrial translation * Trypanosoma brucei * EF-Tu Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.026, year: 2013

  1. The surface-associated elongation factor Tu is concealed for antibody binding on viable pneumococci and meningococci

    Czech Academy of Sciences Publication Activity Database

    Kolberg, J.; Hammerschmidt, S.; Frank, R.; Jonák, Jiří; Šanderová, Hana; Aase, A.

    2008-01-01

    Roč. 53, č. 2 (2008), s. 222-230 ISSN 0928-8244 R&D Projects: GA AV ČR KJB500520503 Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z50200510 Keywords : Streptococcus pneumoniae * Neisseria meningitidis * elongation factor EF-Tu Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.972, year: 2008

  2. Rodents from the Upper Miocene Tuğlu Formation (Çankırı Basin, Central Anatolia, Turkey)

    NARCIS (Netherlands)

    Joniak, Peter; de Bruijn, Hans

    2015-01-01

    The upper Miocene assemblages of rodents collected from two layers of the type section of the Tuğlu Formation (Çankırı Basin, Central Anatolia, Turkey) are described. The assemblage from the lower level is considerably less diverse than that from the upper level. It contains Progonomys together with

  3. 250 kV 6 mA compact Cockcroft-Walton high-voltage power supply

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Zhan-Wen; Su, Xiao-Dong; Wei, Zhen; Huang, Zhi-Wu; Miao, Tian-You; Su, Tong-Ling [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Lu, Xiao-Long; Wang, Jun-Run; Yao, Ze-En, E-mail: zeyao@lzu.edu.cn [School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000 (China); Engineering Research Center for Neutron Application, Ministry of Education, Lanzhou University, Lanzhou 730000 (China)

    2016-08-15

    A compact power supply system for a compact neutron generator has been developed. A 4-stage symmetrical Cockcroft-Walton circuit is adopted to produce 250 kV direct current high-voltage. A 2-stage 280 kV isolation transformer system is used to drive the ion source power supply. For a compact structure, safety, and reliability during the operation, the Cockcroft-Walton circuit and the isolation transformer system are enclosed in an epoxy vessel containing the transformer oil whose size is about ∅350 mm × 766 mm. Test results indicate that the maximum output voltage of the power supply is 282 kV, and the stability of the output voltage is better than 0.63% when the high voltage power supply is operated at 250 kV, 6.9 mA with the input voltage varying ±10%.

  4. Kv4.2 knockout mice display learning and memory deficits in the Lashley maze [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Gregory D. Smith

    2016-10-01

    Full Text Available Background: Potassium channels have been shown to be involved in neural plasticity and learning. Kv4.2 is a subunit of the A-type potassium channel. Kv4.2 channels modulate excitability in the dendrites of pyramidal neurons in the cortex and hippocampus. Deletion of Kv4.2 results in spatial learning and conditioned fear deficits; however, previous studies have only examined deletion of Kv4.2 in aversive learning tests. Methods: For the current study, we used the Lashley maze as an appetitive learning test. We examined Kv4.2 wildtype (WT and knockout (KO mice in the Lashley maze over 4 days during adulthood. The first day consisted of habituating the mice to the maze. The mice then received five trials per day for the next 3 days. The number of errors and the time to the goal box was recorded for each trial. The goal box contained a weigh boat with an appetitive reward (gelatin with sugar. There was an intertrial interval of 15 minutes. Results: We found that Kv4.2 KO mice committed more errors across the trials compared to the WT mice p<0.001. There was no difference in the latency to find the goal box over the period. Discussion: Our finding that deletion of Kv4.2 resulted in more errors in the Lashley maze across 15 trials contribute to a growing body of evidence that Kv4.2 channels are significantly involved in learning and memory.

  5. Kv4.2 knockout mice display learning and memory deficits in the Lashley maze [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Gregory D. Smith

    2017-02-01

    Full Text Available Background: Potassium channels have been shown to be involved in neural plasticity and learning. Kv4.2 is a subunit of the A-type potassium channel. Kv4.2 channels modulate excitability in the dendrites of pyramidal neurons in the cortex and hippocampus. Deletion of Kv4.2 results in spatial learning and conditioned fear deficits; however, previous studies have only examined deletion of Kv4.2 in aversive learning tests. Methods: For the current study, we used the Lashley maze as an appetitive learning test. We examined Kv4.2 wildtype (WT and knockout (KO mice in the Lashley maze over 4 days during adulthood. The first day consisted of habituating the mice to the maze. The mice then received five trials per day for the next 3 days. The number of errors and the time to the goal box was recorded for each trial. The goal box contained a weigh boat with an appetitive reward (gelatin with sugar. There was an intertrial interval of 15 minutes. Results: We found that Kv4.2 KO mice committed more errors across the trials compared to the WT mice p<0.001. There was no difference in the latency to find the goal box over the period. Discussion: Our finding that deletion of Kv4.2 resulted in more errors in the Lashley maze across 15 trials contribute to a growing body of evidence that Kv4.2 channels are significantly involved in learning and memory.

  6. A guidelines handbook for GPR surveys in tunnels: a COST Action TU1208 contribution

    Science.gov (United States)

    Bianchini Ciampoli, Luca; Alani, Amir M.; Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    A significant open issue concerning the reliability of geophysical methods and in particular of ground penetrating radar (GPR), both in research and professional context, is a general lack of international standards. This is a major problem to be faced, in order to gain scientific strictness for the GPR practices, and to easily extend to the international community the results achieved within the area of single virtuous countries. Producing international guidelines can represent an important step forward, in this sense. In the memorandum of understanding of the COST Action TU1208 is clearly stated that one of the main purposes of the Action is the "development of innovative protocols and guidelines which will be published in a handbook and constitute a basis for European Standards, for an effective GPR application in CE tasks; safety, economic and financial criteria will be integrated within the protocols". Of course this is not a simple task to be accomplished. Firstly, survey procedures are highly dependent on the objective of the survey itself. On the basis of the objective of each geophysical test, the GPR system, the antenna configuration, and even the processing procedures may change. Besides, these procedures are also influenced by the environmental conditions in which the tests are performed. This affects several aspects spanning from hardware to software, but including, for instance, also safety issues. Due to these reasons, one of the main goal of the COST Action TU1208 is the development of several guidelines related to the main applications of GPR in the field of civil engineering. In this work, the structure of a guidelines handbook for GPR activities in tunnels is outlined. In the first sections, the principal references in the field are provided, and the most common GPR equipment and complementary technologies are described. Subsequently, the survey methodologies are explained. Particular attention is paid to the preliminary activities to be carried

  7. The Song of the Sabia in the 3rd. Festival Internacional da Canção: boo and sunset of the Tom Jobim bossa nova`s aesthetics

    Directory of Open Access Journals (Sweden)

    Fabio Guilherme Poletto

    2015-07-01

    Full Text Available This article discusses the Song of the Sabia, composition of Tom Jobim and Chico Buarque. Mobilizes different series of sources in order to reconstruct historically the boo to the performance of the song during the III International Song Festival, held in 1968 in the city of Rio de Janeiro. Seeks to highlight how this episode catalyzed the existence of different expectations on the popular song and its role as a vehicle for ideals of modernity amid the interference of the authoritarian environment in the cultural and political field. Then, based on the analysis of the constitutive parameters of the song, suggests possibilities for understanding its musical and poetic construction in critical dialogue with its reverberations in cultural terms.This article discusses the Song of the Sabia, composition of Tom Jobim and Chico Buarque. Mobilizes different series of sources in order to reconstruct historically the boo to the performance of the song during the III International Song Festival, held in 1968 in the city of Rio de Janeiro. Seeks to highlight how this episode catalyzed the existence of different expectations on the popular song and its role as a vehicle for ideals of modernity amid the interference of the authoritarian environment in the cultural and political field. Then, based on the analysis of the constitutive parameters of the song, suggests possibilities for understanding its musical and poetic construction in critical dialogue with its reverberations in cultural terms.

  8. Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells

    Directory of Open Access Journals (Sweden)

    Bo Hyung Lee

    2014-01-01

    Full Text Available The KCNQ gene family, whose members encode Kv7 channels, belongs to the voltage-gated potassium (Kv channel group. The roles of this gene family have been widely investigated in nerve and muscle cells. In the present study, we investigated several characteristics of Kv7.5, which is strongly expressed in the canine osteosarcoma cell line, CCL-183. Serum starvation upregulated Kv7.5 expression, and the Kv7 channel opener, flupirtine, attenuated cell proliferation by arresting cells in the G0/G1 phase. We also showed that Kv7.5 knockdown helps CCL-183 cells to proliferate. In an effort to find an endogenous regulator of Kv7.5, we used mithramycin A to reduce the level of the transcription factor Sp1, and it strongly inhibited the induction of Kv7.5 in CCL-183 cells. These results suggest that the activation of Kv7.5 by flupirtine may exert an anti-proliferative effect in canine osteosarcoma. Therefore, Kv7.5 is a possible molecular target for canine osteosarcoma therapy.

  9. Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL.

    Science.gov (United States)

    Yang, Yong; Wang, Yan-Fu; Yang, Xiao-Fang; Wang, Zhao-Hui; Lian, Yi-Tian; Yang, Ying; Li, Xiao-Wei; Gao, Xiang; Chen, Jian; Shu, Yan-Wen; Cheng, Long-Xian; Liao, Yu-Hua; Liu, Kun

    2013-01-01

    Cholesterol-metabolism-associated molecules, including scavenger receptor class A (SR-A), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), CD36, ACAT1, ABCA1, ABCG1, and scavenger receptor class B type I, can modulate cholesterol metabolism in the transformation from macrophages to foam cells. Voltage-gated potassium channel Kv1.3 has increasingly been demonstrated to play an important role in the modulation of macrophage function. Here, we investigate the role of Kv1.3 in modulating cholesterol-metabolism-associated molecules in human acute monocytic leukemia cell-derived macrophages (THP-1 macrophages) and human monocyte-derived macrophages exposed to oxidized LDL (ox-LDL). Human Kv1.3 and Kv1.5 channels (hKv1.3 and hKv1.5) are expressed in macrophages and form a heteromultimeric channel. The hKv1.3-E314 antibody that we had generated as a specific hKv1.3 blocker inhibited outward delayed rectifier potassium currents, whereas the hKv1.5-E313 antibody that we had generated as a specific hKv1.5 blocker failed. Accordingly, the hKv1.3-E314 antibody reduced percentage of cholesterol ester and enhanced apoA-I-mediated cholesterol efflux in THP-1 macrophages and human monocyte-derived macrophages exposed to ox-LDL. The hKv1.3-E314 antibody downregulated SR-A, LOX-1, and ACAT1 expression and upregulated ABCA1 expression in THP-1 macrophages and human monocyte-derived macrophages. Our results reveal that specific Kv1.3 blockade represents a novel strategy modulating cholesterol metabolism in macrophages, which benefits the treatment of atherosclerotic lesions.

  10. Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL[S

    Science.gov (United States)

    Yang, Yong; Wang, Yan-Fu; Yang, Xiao-Fang; Wang, Zhao-Hui; Lian, Yi-Tian; Yang, Ying; Li, Xiao-Wei; Gao, Xiang; Chen, Jian; Shu, Yan-Wen; Cheng, Long-Xian; Liao, Yu-Hua; Liu, Kun

    2013-01-01

    Cholesterol-metabolism-associated molecules, including scavenger receptor class A (SR-A), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), CD36, ACAT1, ABCA1, ABCG1, and scavenger receptor class B type I, can modulate cholesterol metabolism in the transformation from macrophages to foam cells. Voltage-gated potassium channel Kv1.3 has increasingly been demonstrated to play an important role in the modulation of macrophage function. Here, we investigate the role of Kv1.3 in modulating cholesterol-metabolism-associated molecules in human acute monocytic leukemia cell-derived macrophages (THP-1 macrophages) and human monocyte-derived macrophages exposed to oxidized LDL (ox-LDL). Human Kv1.3 and Kv1.5 channels (hKv1.3 and hKv1.5) are expressed in macrophages and form a heteromultimeric channel. The hKv1.3-E314 antibody that we had generated as a specific hKv1.3 blocker inhibited outward delayed rectifier potassium currents, whereas the hKv1.5-E313 antibody that we had generated as a specific hKv1.5 blocker failed. Accordingly, the hKv1.3-E314 antibody reduced percentage of cholesterol ester and enhanced apoA-I-mediated cholesterol efflux in THP-1 macrophages and human monocyte-derived macrophages exposed to ox-LDL. The hKv1.3-E314 antibody downregulated SR-A, LOX-1, and ACAT1 expression and upregulated ABCA1 expression in THP-1 macrophages and human monocyte-derived macrophages. Our results reveal that specific Kv1.3 blockade represents a novel strategy modulating cholesterol metabolism in macrophages, which benefits the treatment of atherosclerotic lesions. PMID:23099443

  11. DNA Barcoding for Identification of "Candidatus Phytoplasmas" Using a Fragment of the Elongation Factor Tu Gene

    DEFF Research Database (Denmark)

    Makarova, Olga; Contaldo, Nicoletta; Paltrinieri, Samanta

    2012-01-01

    Background Phytoplasmas are bacterial phytopathogens responsible for significant losses in agricultural production worldwide. Several molecular markers are available for identification of groups or strains of phytoplasmas. However, they often cannot be used for identification of phytoplasmas from...... different groups simultaneously or are too long for routine diagnostics. DNA barcoding recently emerged as a convenient tool for species identification. Here, the development of a universal DNA barcode based on the elongation factor Tu (tuf) gene for phytoplasma identification is reported. Methodology....../Principal Findings We designed a new set of primers and amplified a 420–444 bp fragment of tuf from all 91 phytoplasmas strains tested (16S rRNA groups -I through -VII, -IX through -XII, -XV, and -XX). Comparison of NJ trees constructed from the tuf barcode and a 1.2 kbp fragment of the 16S ribosomal gene revealed...

  12. “En tu casa o en la mía’: the interview as infotainment

    Directory of Open Access Journals (Sweden)

    Marián Alonso González

    2016-11-01

    Full Text Available The fight for viewers has led to a spectacular range of television content on offer where boundaries of genres have disappeared giving rise to new formats of hybrid television programmes. This has given a new lease of life to interviews as a genre of infotainment. The present article analyses the programme ‘En tu casa o en la mía’, a new type of interview of famous personalities which combines information and entertainment with elements that are very similar to the info-show. We shall embark on a study of this media product, its narrative, treatment, structure and the technique used so as to describe the elements that make it an example of gender hybridization in today’s television programmes.

  13. Joint efforts to harmonize sound insulation descriptors and classification schemes in Europe (COST TU0901)

    DEFF Research Database (Denmark)

    Rasmussen, Birgit

    2010-01-01

    Sound insulation descriptors, regulatory requirements and classification schemes in Europe represent a high degree of diversity. One implication is very little exchange of experience of housing design and construction details for different levels of sound insulation; another is trade barriers...... for building systems and products. Unfortunately, there is evidence for a development in the "wrong" direction. For example, sound classification schemes for dwellings exist in nine countries. There is no sign on increasing harmonization, rather the contrary, as more countries are preparing proposals with new......, new housing must meet the needs of the people and offer comfort. Also for existing housing, sound insulation aspects should be taken into account, when renovating housing; otherwise the renovation is not “sustainable”. A joint European Action, COST TU0901 "Integrating and Harmonizing Sound Insulation...

  14. Housing for the poor: Implementation and impact of Tu Casa program in Tijuana

    Directory of Open Access Journals (Sweden)

    Ana Elizabeth Jardón Hernández

    2009-01-01

    Full Text Available This study assesses the operation and some major results of most important federal housing program focus on households in poverty situation, with particular emphasis on their instrumentation process and impacts in Tijuana’s housing problem for poor families. The exam aims to analyses the design changes of the program that forced its transformation from Vivah to Tu Casa, and the operational difficulties for a proper implementation in an urban context, as well as its limited impact for solving popular housing needs. It also seeks to weigh the importance of government subsidies to acquire or improve housing, in their progressive modality, as an alternative for social segments excluded from any other public or private financing option.

  15. A avaliação da concordância verbal com o pronome tu em Florianópolis

    Directory of Open Access Journals (Sweden)

    Christiane Maria Nunes de Souza

    2015-09-01

    Full Text Available http://dx.doi.org/10.5007/1984-8420.2015v16n1p170 Neste artigo, com base nos pressupostos da Sociolinguística Variacionista (WEINREICH, LABOV, HERZOG, 1968; LABOV, 1972, 1982, 1994, 2001, 2010, buscamos indícios de como o fenômeno variável de concordância verbal com o pronome tu (tu/Ø falas ~ tu/Ø fala, tu/Ø  falaste ~ tu/Ø falasse ~ tu/Ø falou é avaliado na cidade de Florianópolis. Com essa finalidade, aplicamos um teste de avaliação a 22 alunos do curso de Economia da Universidade Federal de Santa Catarina (UFSC. Os resultados evidenciam uma correlação entre a diminuição das taxas percentuais de concordância verificada em Florianópolis (LOREGIAN-PENKAL, 2004; DAVET, 2013 e uma avaliação positiva/neutra da não marcação da concordância.

  16. Remodeling of Kv1.5 channel in right atria from Han Chinese patients with atrial fibrillation.

    Science.gov (United States)

    Ou, Xian-hong; Li, Miao-ling; Liu, Rui; Fan, Xin-rong; Mao, Liang; Fan, Xue-hui; Yang, Yan; Zeng, Xiao-rong

    2015-04-28

    The incidence of atrial fibrillation (AF) in rheumatic heart diseases (RHD) is very high and increases with age. Occurrence and maintenance of AF are very complicated process accompanied by many different mechanisms. Ion-channel remodeling, including the voltage-gated potassium channel Kv1.5, plays an important role in the pathophysiology of AF. However, the changes of Kv1.5 channel expression in Han Chinese patients with RHD and AF remain poorly understood. The aim of the present study was to investigate whether the Kv1.5 channels of the right atria may be altered with RHD, age, and sex to contribute to AF. Right atrial appendages were obtained from 20 patients with normal cardiac functions who had undergone surgery, and 26 patients with AF. Subjects were picked from 4 groups: adult and aged patients in normal sinus rhythm (SR) and AF. Patients were divided into non-RHD and RHD groups or men and women groups in normal SR and AF, respectively. The expression of Kv1.5 protein and messenger RNA (mRNA) were measured using Western blotting and polymerase chain reaction (PCR) method, respectively. Compared with the SR group, the expression of Kv1.5 protein decreased significantly in the AF group. However, neither Kv1.5 protein nor KCNA5 mRNA had significant differences in adult and aged groups, non-RHD and RHD group, and men and women group of AF. The expression of Kv1.5 channel protein changes with AF but not with age, RHD, and sex in AF.

  17. Dosimetry in phantom of newborn head for computerized tomography signs with voltages of 80, 100 and 120 KV

    International Nuclear Information System (INIS)

    Oliveira, G.A.P.; Mourão, A.P.

    2017-01-01

    Computed Tomography is the radiodiagnostic method that most contributes to the dose deposition in population. Therefore, the dose reductions used in these tests are very important, especially for pediatric patients who have a life expectancy greater than the rest of the population. This study purpose to compare the doses generated from newborns (NB) with different voltages in a 64-channel multichannel CT equipment of the GE brand. One head phantom in a cylindrical shape made in PMMA were used to newborn patient dimensions. 100 mA.s of charge of the X-ray tube were standardized, alternating the voltages between 80, 100, 120 kV in the axial acquisition. The absorbed dose measurements were performed with a pencil-type ionization chamber positioned within the five apertures in the phantom. The phantom was developed with the cephalic percentile of male NB of 14 and female of 28 days, respectively. The doses obtained in the head phantom of NB were compared with the voltages of 80, 100 and 120 kV. The volumetric dose index, C VOL , generated in the 120 kV protocol was 25.10 mGy, for 100 kV of 19,06 mGy and not for 80 kV 15,81 mGy. The results allow to evaluate that for the generation of images with 120 kV, the dose was 37.0% higher when compared to the voltage of 80 kV. The study shows that the increase in tension in the tomography protocols also makes it possible to increase the dose for the NB patient. (author)

  18. Dosimetry in phantom of newborn head for computerized tomography signs with voltages of 80, 100 and 120 KV

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, G.A.P.; Mourão, A.P., E-mail: giovanni.paiva@pbh.gov.br, E-mail: apratabhz@gmail.com [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear

    2017-07-01

    Computed Tomography is the radiodiagnostic method that most contributes to the dose deposition in population. Therefore, the dose reductions used in these tests are very important, especially for pediatric patients who have a life expectancy greater than the rest of the population. This study purpose to compare the doses generated from newborns (NB) with different voltages in a 64-channel multichannel CT equipment of the GE brand. One head phantom in a cylindrical shape made in PMMA were used to newborn patient dimensions. 100 mA.s of charge of the X-ray tube were standardized, alternating the voltages between 80, 100, 120 kV in the axial acquisition. The absorbed dose measurements were performed with a pencil-type ionization chamber positioned within the five apertures in the phantom. The phantom was developed with the cephalic percentile of male NB of 14 and female of 28 days, respectively. The doses obtained in the head phantom of NB were compared with the voltages of 80, 100 and 120 kV. The volumetric dose index, C{sub VOL}, generated in the 120 kV protocol was 25.10 mGy, for 100 kV of 19,06 mGy and not for 80 kV 15,81 mGy. The results allow to evaluate that for the generation of images with 120 kV, the dose was 37.0% higher when compared to the voltage of 80 kV. The study shows that the increase in tension in the tomography protocols also makes it possible to increase the dose for the NB patient. (author)

  19. SU-E-T-163: Thin-Film Organic Photocell (OPV) Properties in MV and KV Beams for Dosimetry Applications.

    Science.gov (United States)

    Ng, S K; Hesser, J; Zhang, H; Gowrisanker, S; Yakushevich, S; Shulhevich, Y; Abkai, C; Wack, L; Zygmanski, P

    2012-06-01

    To characterize dosimetric properties of low-cost thin film organic-based photovoltaic (OPV) cells to kV and MV x-ray beams for their usage as large area dosimeter for QA and patient safety monitoring device. A series of thin film OPV cells of various areas and thicknesses were irradiated with MV beams to evaluate the stability and reproducibility of their response, linearity and sensitivity to absorbed dose. The OPV response to x-rays of various linac energies were also characterized. Furthermore the practical (clinical) sensitivity of the cells was determined using IMRT sweeping gap test generated with various gap sizes. To evaluate their potential usage in the development of low cost kV imaging device, the OPV cells were irradiated with kV beam (60-120 kVp) from a fluoroscopy unit. Photocell response to the absorbed dose was characterized as a function of the organic thin film thickness and size, beam energy and exposure for kV beams as well. In addition, photocell response was determined with and without thin plastic scintillator. Response of the OPV cells to the absorbed dose from kV and MV beams are stable and reproducible. The photocell response was linearly proportional to the size and about slightly decreasing with the thickness of the organic thin film, which agrees with the general performance of the photocells in visible light. The photocell response increases as a linear function of absorbed dose and x-ray energy. The sweeping gap tests performed showed that OPV cells have sufficient practical sensitivity to measured MV x-ray delivery with gap size as small as 1 mm. With proper calibration, the OPV cells could be used for online radiation dose measurement for quality assurance and patient safety purposes. Their response to kV beam show promising potential in development of low cost kV radiation detection devices. © 2012 American Association of Physicists in Medicine.

  20. FPGA based control system for -100 kV, 25 A Crowbarless DC power supply

    International Nuclear Information System (INIS)

    Upadhyay, R.; Tripathi, A.; Badapanda, M.K.; Lad, M.

    2015-01-01

    FPGA based digital control system has been developed for -100 kV, 25 A solid state modular crowbarless DC klystron bias power supply of 1 MW, 352.2 MHz RF test stand. The control system has capability to operate this power supply either in CW or pulse mode. Central controller, PSM controller and graphical user interface are key parts of this control system. Central controller monitors the status of various subsystems of this power supply like 11 kV step start unit, four numbers of main transformers each having 44 numbers of secondary windings and 176 numbers of switch power modules for deciding the number of power modules to be put ON and their duty cycles depending on the set output voltage and current. PSM controller sends appropriate control signal to the switch power modules through fibre optic lines and communicates it to the central controller. Linux based graphical user interface has been developed which enables the user to set the operating parameters along with their trip limits and displays the information of critical parameters of this power supply on a local touch screen panel. Provision for remote control and supervision is also provided through a separate PC connected to the main control system via Ethernet. The control system has capability to trip the power supply within 5 μsec in case any parameter exceeds its set limit. Suitable data logging feature is incorporated for offline fault analysis. The control system architecture along with its software protection interlocks are presented in this paper. The performance of the control system has been verified during operation of -100 kV, 25 A DC power supply with 1 MW, 352.2 MHz klystron amplifier. (author)

  1. Development of 10 kV 4H-SiC JBS diode with FGR termination

    International Nuclear Information System (INIS)

    Huang Runhua; Tao Yonghong; Cao Pengfei; Wang Ling; Li Rui; Chen Gang; Bai Song; Li Yun; Zhao Zhifei

    2014-01-01

    The design, fabrication, and electrical characteristics of the 4H-SiC JBS diode with a breakdown voltage higher than 10 kV are presented. 60 floating guard rings have been used in the fabrication. Numerical simulations have been performed to select the doping level and thickness of the drift layer and the effectiveness of the edge termination technique. The n-type epilayer is 100 μm in thickness with a doping of 6 × 10 14 cm −3 . The on-state voltage was 2.7 V at J F = 13 A/cm 2 . (semiconductor devices)

  2. Back-to-Back Energization of a 60kV Cable Network - Inrush Currents Phenomenon

    DEFF Research Database (Denmark)

    Faria da Silva, Filipe; Bak, Claus Leth; Hansen, M. Lind

    2010-01-01

    On November 2008 the Danish government decided that all Danish transmission lines with a rated voltage equal to and below 150kV must be put underground, in order to reduce the visual pollution caused by Overhead Lines. This decision will lead to a massive use of underground cables in the Danish...... Network, and force a change in the approach used until now when planning, analyzing and operating electrical power systems. One problem that might arise is the energization of cables in parallel, as this operation may originate high inrush currents, which represent a risk to the circuit breakers connected...

  3. On low-resistance neutral earthing mode in 20 kV overhead and cable networks

    Directory of Open Access Journals (Sweden)

    Mayorov Andrey

    2017-01-01

    Full Text Available The problems of justification and selection of the required single-phase short-circuit current values in cable and overhead networks of 20 kV with low-resistance neutral earthing are considered. It is shown that the desired values of the short-circuit current can be determined on the basis of harmonization of conflicting influencing factors: reliability of the relay protection and automation devices and required resistances of the earthing devices of electrical installations, including personnel safety. In this case, the main influencing factor is the electrical network structure and parameters

  4. Operating characteristics and modeling of the LLNL 100-kV electric gun

    International Nuclear Information System (INIS)

    Osher, J.E.; Barnes, G.; Chau, H.H.; Lee, R.S.; Lee, C.; Speer, R.; Weingart, R.C.

    1989-01-01

    In the electric gun, the explosion of an electrically heated metal foil and the accompanying magnetic forces drive a thin flyer plate up a short barrel. Flyer velocities of up to 18 km/s make the gun useful for hypervelocity impact studies. The authors briefly review the technological evolution of the exploding-metal circuit elements that power the gun, describe the 100-kV electric gun designed at Lawrence Livermore National Laboratory (LLNL) in some detail, and present the general principles of electric gun operation. They compare the experimental performance of the LLNL gun with a simple model and with predictions of a magnetohydrodynamics code

  5. Calculation of the electron trajectory for 200 kV self-shielded electron accelerator

    International Nuclear Information System (INIS)

    Wang Shuiqing

    2000-01-01

    In order to calculate the electron trajectory of 200 kV self-shielded electron accelerator, the electric field is calculated with a TRAJ program. In this program, following electron track mash points one by one, the electron beam trajectories are calculated. Knowing the effect of grid voltage on electron optics and gaining grid voltage focusing effect in the various energy grades, the authors have gained scientific basis for adjusting grid voltage, and also accumulated a wealth of experience for designing self-shielded electron accelerator or electron curtain in future

  6. PLTS Transformerless Tegangan 20 kV menggunakan Cascaded H-Bridge Multilevel Inverter

    Directory of Open Access Journals (Sweden)

    ANGGARA BRAJAMUSTHI

    2018-03-01

    Full Text Available ABSTRAK Aplikasi dari inverter multilevel pada sistem Pusat Listrik Tenaga Surya (PLTS dapat menghilangkan kebutuhan terhadap transformator, sehingga dapat mengurangi biaya investasi, mengurangi kompleksitas instalasi dan menghilangkan rugi-rugi daya transformator. Pada penelitian ini, sebuah inverter dengan topologi Cascaded H-Bridge Multilevel Inverter dirancang agar mampu mengubah tegangan rendah DC dari beberapa Photovoltaic (PV array menjadi tegangan fasa-fasa 20 kV AC. Perancangan menghasilkan sebuah inverter 3 fasa 27-level dimana setiap level masing-masing memiliki PV array, DC-DC boost converter, H-bridge inverter, dan keluaran 3 fasa terhubung dengan filter LCL. Setiap komponen dari inverter dan sistem tersebut kemudian dimodelkan pada MATLAB Simulink untuk mensimulasikan kinerja dari setiap komponen dan sistem pada Standard Test Condition (STC dari modul PV. Pada keadaan STC, daya 3 fasa maksimum yang dapat dihasilkan adalah 1,716 MW atau 68,54% dari daya DC maksimum sebesar 2,5 MWp. Sistem dapat menghasilkan tegangan fasa-fasa keluaran sebesar 20 kV dengan Total Harmonic Distortion (THD di bawah 5%. Kata kunci: Pusat Listrik Tenaga Surya (PLTS, photovoltaic, Cascaded H-Bridge Multilevel Inverter ABSTRACT The application of Multilevel Inverter in a Photovoltaic Solar Power Plant system could eliminate the needs of step-up transformer, which will reduce the system investment cost, simplify the system installation and also eliminate power losses of the transformer. In this paper, an inverter design was proposed with Cascaded H-Bridge Multilevel Inverter topology that is capable of converting low voltage DC power from several PV arrays into 20 kV AC power. The design resulted a 3 phase 27-level inverter where each level in the inverter has its own photovoltaic array, DC-DC boost converter, H-bridge inverter, and the 3 phase output is connected to LCL filter. Each component of the Inverter and the system were then modelled in MATLAB

  7. WE-G-18A-02: Calibration-Free Combined KV/MV Short Scan CBCT

    Energy Technology Data Exchange (ETDEWEB)

    Wu, M; Loo, B; Bazalova, M; Fahrig, R [Stanford University, Stanford, CA (United States); Star-Lack, J [Varian Medical Systems, Palo Alto, CA (United States)

    2014-06-15

    Purpose: To combine orthogonal kilo-voltage (kV) and Mega-voltage (MV) projection data for short scan cone-beam CT to reduce imaging time on current radiation treatment systems, using a calibration-free gain correction method. Methods: Combining two orthogonal projection data sets for kV and MV imaging hardware can reduce the scan angle to as small as 110° (90°+fan) such that the total scan time is ∼18 seconds, or within a breath hold. To obtain an accurate reconstruction, the MV projection data is first linearly corrected using linear regression using the redundant data from the start and end of the sinogram, and then the combined data is reconstructed using the FDK method. To correct for the different changes of attenuation coefficients in kV/MV between soft tissue and bone, the forward projection of the segmented bone and soft tissue from the first reconstruction in the redundant region are added to the linear regression model. The MV data is corrected again using the additional information from the segmented image, and combined with kV for a second FDK reconstruction. We simulated polychromatic 120 kVp (conventional a-Si EPID with CsI) and 2.5 MVp (prototype high-DQE MV detector) projection data with Poisson noise using the XCAT phantom. The gain correction and combined kV/MV short scan reconstructions were tested with head and thorax cases, and simple contrast-to-noise ratio measurements were made in a low-contrast pattern in the head. Results: The FDK reconstruction using the proposed gain correction method can effectively reduce artifacts caused by the differences of attenuation coefficients in the kV/MV data. The CNRs of the short scans for kV, MV, and kV/MV are 5.0, 2.6 and 3.4 respectively. The proposed gain correction method also works with truncated projections. Conclusion: A novel gain correction and reconstruction method was developed to generate short scan CBCT from orthogonal kV/MV projections. This work is supported by NIH Grant 5R01CA138426-05.

  8. SU-F-J-16: Planar KV Imaging Dose Reduction Study

    Energy Technology Data Exchange (ETDEWEB)

    Gershkevitsh, E; Zolotuhhin, D [North Estonia Medical Centre, Tallinn (Estonia)

    2016-06-15

    Purpose: IGRT has become an indispensable tool in modern radiotherapy with kV imaging used in many departments due to superior image quality and lower dose when compared to MV imaging. Many departments use manufacturer supplied protocols for imaging which are not always optimised between image quality and radiation dose (ALARA). Methods: Whole body phantom PBU-50 (Kyoto Kagaku ltd., Japan) for imaging in radiology has been imaged on Varian iX accelerator (Varian Medical Systems, USA) with OBI 1.5 system. Manufacturer’s default protocols were adapted by modifying kV and mAs values when imaging different anatomical regions of the phantom (head, thorax, abdomen, pelvis, extremities). Images with different settings were independently reviewed by two persons and their suitability for IGRT set-up correction protocols were evaluated. The suitable images with the lowest mAs were then selected. The entrance surface dose (ESD) for manufacturer’s default protocols and modified protocols were measured with RTI Black Piranha (RTI Group, Sweden) and compared. Image quality was also measured with kVQC phantom (Standard Imaging, USA) for different protocols. The modified protocols have been applied for clinical work. Results: For most cases optimized protocols reduced the ESD on average by a factor of 3(range 0.9–8.5). Further reduction in ESD has been observed by applying bow-tie filter designed for CBCT. The largest reduction in dose (12.2 times) was observed for Thorax lateral protocol. The dose was slightly increased (by 10%) for large pelvis AP protocol. Conclusion: Manufacturer’s default IGRT protocols could be optimised to reduce the ESD to the patient without losing the necessary image quality for patient set-up correction. For patient set-up with planar kV imaging the bony anatomy is mostly used and optimization should focus on this aspect. Therefore, the current approach with anthropomorphic phantom is more advantageous in optimization over standard kV quality

  9. A 350 KV nanosecond pulse voltage generator with adjustable pulsed-width

    International Nuclear Information System (INIS)

    Wang, X.; Wang, M.; Chen, Y.Q.; Zeng, L.G.; Han, M.

    2002-01-01

    This paper presents a 350 kV nanosecond pulse voltage generator (NPVG). The voltage pulsed-width can be adjusted from 30 to 160 ns. The generator consists of: Marx generator, pulsed forming line (PFL), main switch and matched impedance. The output voltage of Marx generator is over than nU c (n- the stage number of Marx generator, U c -the charging voltage of capacitor). When the pulse forming line is terminated with an impedance that is over than the characteristic impedance of PFL, the higher voltage pulse was provided for the load

  10. Scale model test on a novel 400 kV double-circuit composite pylon

    DEFF Research Database (Denmark)

    Wang, Qian; Bak, Claus Leth; Silva, Filipe Miguel Faria da

    This paper investigates lightning shielding performance of a novel 400 kV double-circuit composite pylon, with the method of scale model test. Lightning strikes to overhead lines were simulated by long-gap discharges between a high voltage electrode with an impulse voltage and equivalent conductors...... around the pylon is discussed. Combined test results and striking distance equation in electro-geometric model, the approximate maximum lightning current that can lead to shielding failure is calculated. Test results verify that the unusual negative shielding angle of - 60° in the composite pylon meets...... requirement and the shielding wires provide acceptable protection from lightning strikes....

  11. Development of a high current 250 kV photocathode dc gun

    International Nuclear Information System (INIS)

    Nishimori, Nobuyuki; Nagai, Ryoji; Sawamura, Masaru; Hajima, Ryoichi

    2016-01-01

    We have developed a high current photocathode dc gun at JAEA for the next generation light sources such as an energy recovery linac and high-repetition rate X-ray free electron laser. The gun is equipped with a multialkali photocathode preparation system. Quantum efficiency of 0.37% at 532 nm was obtained for a Cs_3Sb photocathode. The gun was high voltage conditioned up to 230 kV with a cathode electrode. Beam generation test from the multialkali photocathode will be performed by the end of FY2015. (author)

  12. Off-resonance transformer charging for 250-kV water Blumlein

    International Nuclear Information System (INIS)

    Cook, E.; Reginato, L.

    1978-01-01

    An off-resonance transformer for charging a 250-kV Blumlein system provides a viable alternative to other charging schemes by permitting the use of conventional thyratrons. Such a transformer must have reliability, a reasonable voltage step-up, and a non-reversing primary current. The analysis, design, and performance data for such a transformer are presented. The strong interrelationship between transformer design and Blumlein requirements necessitates that Blumlein description and design criterion be briefly presented prior to transformer design such that transformer load requirements be defined

  13. Pharmacological activation of Kv11.1 in transgenic long QT-1 rabbits

    DEFF Research Database (Denmark)

    Bentzen, Bo Hjorth; Bahrke, Sophia; Wu, Kezhong

    2011-01-01

    Transgenic rabbits expressing pore mutants of K(V)7.1 display a long QT syndrome 1 (LQT1) phenotype. Recently, NS1643 has been described to increase I(Kr).We hypothesized that NS1643 would shorten the action potential duration (APD(90)) in LQT1 rabbits. Transgenic LQT1 rabbits were compared...... with littermate control (LMC) rabbits. In vivo electrocardiogram studies in sedated animals were performed at baseline and during 45 minutes of intravenous infusion of NS1643 or vehicle in a crossover design. Ex vivo monophasic action potentials were recorded from Langendorff-perfused hearts at baseline...

  14. Sidney-North Yuma 230-kV Transmission Line Project, Colorado and Nebraska

    Energy Technology Data Exchange (ETDEWEB)

    1991-06-01

    This report describes the need for a 230-kV overhead transmission line to supply power from Sidney, Nebraska to eastern Colorado. The alternative scenario compared to construction of the line is No Action. Rejected alternatives include underground lines and different routing paths, with a possible extension to the Sterling area. Both scenarios are evaluated for environmental effects, cost, and consequences for the eastern Colorado region. The proposed route is determined to be the environmentally preferred choice. 120 refs., 6 figs., 13 tabs. (MHB)

  15. A 150 kV Isolation Transformer for a Neutron Generator

    International Nuclear Information System (INIS)

    Dechthummarong, C.; Pratumtip, P.; Thongleurm, C.; Vichaimongkol, P.; Charoennugul, R.; Vilaithong, T.

    1998-01-01

    The work aims at the design and construction of a 150 kV isolation transformer for a neutron generator. The transformer windings are designed to use cylindrical layers with circular enamel copper wires. The insulation of the dry type transformer uses the epoxy resin for encapsulated winding. This insulation is non-flammable under temperature 350 degree celsius and the breakdown voltage is 10-18 kV/mm. This insulation is suitable for insulating high voltage. The design of provides the temperature rise of winding not exceeding 65 degree celsius for protection of the cracking of epoxy resin due to the expansion of winding

  16. The influence of the 220 kv and 66 kv power electric systems and the subsystem of their servitude range in the 'chaco central' ecosystem in Paraguay; Relacion de tendido electrico de 220 kV y 66kV en el ecosistema del chaco central del Paraguay y el subsistema de su franja de servidumbre

    Energy Technology Data Exchange (ETDEWEB)

    Acosta E, Hugo Cesar [ANDE - Administracion Nacional de Electricidad, Assuncion (Paraguay)

    2001-07-01

    According to the report from DOP/EL, in a medium period of time it has been registered nine disconnections of the 66 kv power transmission line located in the Chaco zone occurred in the nocturnal period. Those disconnections affected negatively the milky processes of Colinas Menonitas, deteriorating more than 20,000 liters of milk for each cut of the electric energy service. DMA/IM has accomplished a investigation job together with DOM/FO and has found the main reasons for those disconnections, so based on them it has recommended possible solutions related to the problem.

  17. Outlook for use of 10 kV voltage in coal mines

    Energy Technology Data Exchange (ETDEWEB)

    Voloshchenko, N.I.; Nabokov, E.P.; Razumnyy, Yu.T.

    1983-01-01

    Based on technical-economic studies of the models of the plans for electricity supply, the economic expediency is shown of comprehensive implementation of measures for transition of high-voltage equipment of the mines to voltage of 10 kV with simultaneous improvement in the permissible power of short circuiting to 150 MV X A. Technical problems were examined which are associated with creation of explosion-safe electrical equipment, corresponding to the new technical requirements for the system of electricity supply of the coal mine.

  18. β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses.

    Science.gov (United States)

    Hartmann, Stephanie; Zheng, Fang; Kyncl, Michele C; Karch, Sandra; Voelkl, Kerstin; Zott, Benedikt; D'Avanzo, Carla; Lomoio, Selene; Tesco, Giuseppina; Kim, Doo Y; Alzheimer, Christian; Huth, Tobias

    2018-04-04

    The β-secretase β-site APP-cleaving enzyme 1 (BACE1) is deemed a major culprit in Alzheimer's disease, but accumulating evidence indicates that there is more to the enzyme than driving the amyloidogenic processing of the amyloid precursor protein. For example, BACE1 has emerged as an important regulator of neuronal activity through proteolytic and, most unexpectedly, also through nonproteolytic interactions with several ion channels. Here, we identify and characterize the voltage-gated K + channel 3.4 (Kv3.4) as a new and functionally relevant interaction partner of BACE1. Kv3.4 gives rise to A-type current with fast activating and inactivating kinetics and serves to repolarize the presynaptic action potential. We found that BACE1 and Kv3.4 are highly enriched and remarkably colocalized in hippocampal mossy fibers (MFs). In BACE1 -/- mice of either sex, Kv3.4 surface expression was significantly reduced in the hippocampus and, in synaptic fractions thereof, Kv3.4 was specifically diminished, whereas protein levels of other presynaptic K + channels such as K Ca 1.1 and K Ca 2.3 remained unchanged. The apparent loss of presynaptic Kv3.4 affected the strength of excitatory transmission at the MF-CA3 synapse in hippocampal slices of BACE1 -/- mice when probed with the Kv3 channel blocker BDS-I. The effect of BACE1 on Kv3.4 expression and function should be bidirectional, as predicted from a heterologous expression system, in which BACE1 cotransfection produced a concomitant upregulation of Kv3.4 surface level and current based on a physical interaction between the two proteins. Our data show that, by targeting Kv3.4 to presynaptic sites, BACE1 endows the terminal with a powerful means to regulate the strength of transmitter release. SIGNIFICANCE STATEMENT The β-secretase β-site APP-cleaving enzyme 1 (BACE1) is infamous for its crucial role in the pathogenesis of Alzheimer's disease, but its physiological functions in the intact nervous system are only gradually

  19. Metal artifact correction for x-ray computed tomography using kV and selective MV imaging

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Meng, E-mail: mengwu@stanford.edu [Department of Electrical Engineering, Stanford University, Stanford, California 94305 (United States); Keil, Andreas [microDimensions GmbH, Munich 81379 (Germany); Constantin, Dragos; Star-Lack, Josh [Varian Medical Systems, Inc., Palo Alto, California 94304 (United States); Zhu, Lei [Nuclear and Radiological Engineering and Medical Physics Programs, The George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 (United States); Fahrig, Rebecca [Department of Radiology, Stanford University, Stanford, California 94305 (United States)

    2014-12-15

    Purpose: The overall goal of this work is to improve the computed tomography (CT) image quality for patients with metal implants or fillings by completing the missing kilovoltage (kV) projection data with selectively acquired megavoltage (MV) data that do not suffer from photon starvation. When both of these imaging systems, which are available on current radiotherapy devices, are used, metal streak artifacts are avoided, and the soft-tissue contrast is restored, even for regions in which the kV data cannot contribute any information. Methods: Three image-reconstruction methods, including two filtered back-projection (FBP)-based analytic methods and one iterative method, for combining kV and MV projection data from the two on-board imaging systems of a radiotherapy device are presented in this work. The analytic reconstruction methods modify the MV data based on the information in the projection or image domains and then patch the data onto the kV projections for a FBP reconstruction. In the iterative reconstruction, the authors used dual-energy (DE) penalized weighted least-squares (PWLS) methods to simultaneously combine the kV/MV data and perform the reconstruction. Results: The authors compared kV/MV reconstructions to kV-only reconstructions using a dental phantom with fillings and a hip-implant numerical phantom. Simulation results indicated that dual-energy sinogram patch FBP and the modified dual-energy PWLS method can successfully suppress metal streak artifacts and restore information lost due to photon starvation in the kV projections. The root-mean-square errors of soft-tissue patterns obtained using combined kV/MV data are 10–15 Hounsfield units smaller than those of the kV-only images, and the structural similarity index measure also indicates a 5%–10% improvement in the image quality. The added dose from the MV scan is much less than the dose from the kV scan if a high efficiency MV detector is assumed. Conclusions: The authors have shown that it

  20. Metal artifact correction for x-ray computed tomography using kV and selective MV imaging

    International Nuclear Information System (INIS)

    Wu, Meng; Keil, Andreas; Constantin, Dragos; Star-Lack, Josh; Zhu, Lei; Fahrig, Rebecca

    2014-01-01

    Purpose: The overall goal of this work is to improve the computed tomography (CT) image quality for patients with metal implants or fillings by completing the missing kilovoltage (kV) projection data with selectively acquired megavoltage (MV) data that do not suffer from photon starvation. When both of these imaging systems, which are available on current radiotherapy devices, are used, metal streak artifacts are avoided, and the soft-tissue contrast is restored, even for regions in which the kV data cannot contribute any information. Methods: Three image-reconstruction methods, including two filtered back-projection (FBP)-based analytic methods and one iterative method, for combining kV and MV projection data from the two on-board imaging systems of a radiotherapy device are presented in this work. The analytic reconstruction methods modify the MV data based on the information in the projection or image domains and then patch the data onto the kV projections for a FBP reconstruction. In the iterative reconstruction, the authors used dual-energy (DE) penalized weighted least-squares (PWLS) methods to simultaneously combine the kV/MV data and perform the reconstruction. Results: The authors compared kV/MV reconstructions to kV-only reconstructions using a dental phantom with fillings and a hip-implant numerical phantom. Simulation results indicated that dual-energy sinogram patch FBP and the modified dual-energy PWLS method can successfully suppress metal streak artifacts and restore information lost due to photon starvation in the kV projections. The root-mean-square errors of soft-tissue patterns obtained using combined kV/MV data are 10–15 Hounsfield units smaller than those of the kV-only images, and the structural similarity index measure also indicates a 5%–10% improvement in the image quality. The added dose from the MV scan is much less than the dose from the kV scan if a high efficiency MV detector is assumed. Conclusions: The authors have shown that it

  1. Metal artifact correction for x-ray computed tomography using kV and selective MV imaging.

    Science.gov (United States)

    Wu, Meng; Keil, Andreas; Constantin, Dragos; Star-Lack, Josh; Zhu, Lei; Fahrig, Rebecca

    2014-12-01

    The overall goal of this work is to improve the computed tomography (CT) image quality for patients with metal implants or fillings by completing the missing kilovoltage (kV) projection data with selectively acquired megavoltage (MV) data that do not suffer from photon starvation. When both of these imaging systems, which are available on current radiotherapy devices, are used, metal streak artifacts are avoided, and the soft-tissue contrast is restored, even for regions in which the kV data cannot contribute any information. Three image-reconstruction methods, including two filtered back-projection (FBP)-based analytic methods and one iterative method, for combining kV and MV projection data from the two on-board imaging systems of a radiotherapy device are presented in this work. The analytic reconstruction methods modify the MV data based on the information in the projection or image domains and then patch the data onto the kV projections for a FBP reconstruction. In the iterative reconstruction, the authors used dual-energy (DE) penalized weighted least-squares (PWLS) methods to simultaneously combine the kV/MV data and perform the reconstruction. The authors compared kV/MV reconstructions to kV-only reconstructions using a dental phantom with fillings and a hip-implant numerical phantom. Simulation results indicated that dual-energy sinogram patch FBP and the modified dual-energy PWLS method can successfully suppress metal streak artifacts and restore information lost due to photon starvation in the kV projections. The root-mean-square errors of soft-tissue patterns obtained using combined kV/MV data are 10-15 Hounsfield units smaller than those of the kV-only images, and the structural similarity index measure also indicates a 5%-10% improvement in the image quality. The added dose from the MV scan is much less than the dose from the kV scan if a high efficiency MV detector is assumed. The authors have shown that it is possible to improve the image quality of

  2. Kv1.1 knock-in ataxic mice exhibit spontaneous myokymic activity exacerbated by fatigue, ischemia and low temperature.

    Science.gov (United States)

    Brunetti, Orazio; Imbrici, Paola; Botti, Fabio Massimo; Pettorossi, Vito Enrico; D'Adamo, Maria Cristina; Valentino, Mario; Zammit, Christian; Mora, Marina; Gibertini, Sara; Di Giovanni, Giuseppe; Muscat, Richard; Pessia, Mauro

    2012-09-01

    Episodic ataxia type 1 (EA1) is an autosomal dominant neurological disorder characterized by myokymia and attacks of ataxic gait often precipitated by stress. Several genetic mutations have been identified in the Shaker-like K(+) channel Kv1.1 (KCNA1) of EA1 individuals, including V408A, which result in remarkable channel dysfunction. By inserting the heterozygous V408A, mutation in one Kv1.1 allele, a mouse model of EA1 has been generated (Kv1.1(V408A/+)). Here, we investigated the neuromuscular transmission of Kv1.1(V408A/+) ataxic mice and their susceptibility to physiologically relevant stressors. By using in vivo preparations of lateral gastrocnemius (LG) nerve-muscle from Kv1.1(+/+) and Kv1.1(V408A/+) mice, we show that the mutant animals exhibit spontaneous myokymic discharges consisting of repeated singlets, duplets or multiplets, despite motor nerve axotomy. Two-photon laser scanning microscopy from the motor nerve, ex vivo, revealed spontaneous Ca(2+) signals that occurred abnormally only in preparations dissected from Kv1.1(V408A/+) mice. Spontaneous bursting activity, as well as that evoked by sciatic nerve stimulation, was exacerbated by muscle fatigue, ischemia and low temperatures. These stressors also increased the amplitude of compound muscle action potential. Such abnormal neuromuscular transmission did not alter fiber type composition, neuromuscular junction and vascularization of LG muscle, analyzed by light and electron microscopy. Taken together these findings provide direct evidence that identifies the motor nerve as an important generator of myokymic activity, that dysfunction of Kv1.1 channels alters Ca(2+) homeostasis in motor axons, and also strongly suggest that muscle fatigue contributes more than PNS fatigue to exacerbate the myokymia/neuromyotonia phenotype. More broadly, this study points out that juxtaparanodal K(+) channels composed of Kv1.1 subunits exert an important role in dampening the excitability of motor nerve axons during

  3. Step-by-step application methodology in practical KEPCO 22.9kV bus-bar system

    International Nuclear Information System (INIS)

    Yoon, Jae-young; Lee, Seung-yeol

    2010-01-01

    With the increase of power demand and the progress of power industry deregulation, the transmission and distribution systems will have more complicated problems by the influence of curtailing investment and the NIMBY phenomena in overall power systems. [1-2] It is expected that the route length per MW demand of South Korea will decrease gradually from 0.6[C-km/MW] to 0.53[C-km/MW] in 2010.[3] This comes up to a real serious problem from system planning and operation viewpoints. HTS technologies related to power system have properties to solve these complex transmission and distribution constraints, especially for metropolitan area, in the future. As the HTS technology has developed, the HTS cable technology can be the most effective alternative to solve the future expected power network constraints. This paper describes the application methodology of developing 22.9kV HTS cable by CAST for practical distribution system. 22.9kV HTS cable under development with step-by-step application methodology can substitute the existing and planning conventional 154kV cable.[4-5] If this scheme is applied, part of downtown 154kV substation of metropolitan city such as Seoul can be changed into 22.9kV switching station. Additionally, it can give huge economic, environmental benefits to all of the concerned authorities.

  4. 30 kV/10 mA solid state anode modulator for gyrotron plasma heating: design issues and results

    International Nuclear Information System (INIS)

    Fasel, D.; Lucia, C.; Ganuza, D.; Doyharzabal, I.

    2001-01-01

    Three 30 kV/10 mA solid state pulsed modulators have been delivered to the CRPP in Lausanne, by the company JEMA. Each modulator supplies the anode grid of a triode type gyrotron, used for heating purpose at the third harmonic in the TCV Tokamak. The main parameters of the final design are: the use of solid state technology, a floating output referred to the -80 kV of the gyrotron cathode potential, an output voltage range of -5 to 30 kV, 1 kHz square and sinusoidal modulation, fast switching off to -5 kV (10 μs) and pulsed operation (duty cycle of 1%). After studying and testing a solution based on regulated Mosfet transistors in series, a more stable alternative has been adopted. The final topology consists of a rectifier fed from an insulated 230 V input, a chopper, two inverter steps (for +30 and -5 kV) supplying two diode rectifiers bridges through HV transformers with two switches which commute the load to the positive or negative voltage, connected in series. This article presents the most significant aspects of the design, with special emphasis on the control principle. The final results will be presented in the context of normal operation, supplying a triode gyrotron

  5. Comparison of the quality of the chest film between digital radiography and conventional high kV radiography

    International Nuclear Information System (INIS)

    Zeng Qingsi; Cen Renli; Chen Ling; He Jianxun; Lin Hanfei

    2003-01-01

    Objective: To evaluate the quality and usefulness of direct digital radiography system in roentgenogram of chest in clinical practice. Methods: 1000 cases of chest roentgenograms with digital radiography and high kV conventional radiography were selected for analysis by 3 senior radiologists. Results: 1. With digital radiography system, the quality of chest film was assessed as grade A in 50.6%, grade B in 38.5%, grade C in 10.9%, and no waste film. 2. With conventional high kV radiography, the quality of chest film was assessed as grade A in 41.1%, grade B in 44.1%, grade C in 13.3%, and waste film in 1.5%. The direct digital radiography was statistically superior to the conventional high kV radiography. 3. The fine structure of the lungs could be revealed in 100.0% of chest roentgenogram with direct digital radiograph system, which was significantly higher than that acquired with the conventional high KV radiography (78.6%, P < 0.001). Conclusion: Direct digital radiography could provide the chest film with better quality than that with the conventional high kV radiography. The direct digital radiography system is easy to operate, fast in capturing imaging and could provide post-processing techniques, which will facilitate the accurate diagnosis of chest radiography

  6. Localization and function of the Kv3.1b subunit in the rat medulla oblongata: focus on the nucleus tractus solitarii

    Science.gov (United States)

    Dallas, Mark L; Atkinson, Lucy; Milligan, Carol J; Morris, Neil P; Lewis, David I; Deuchars, Susan A; Deuchars, Jim

    2005-01-01

    The voltage-gated potassium channel subunit Kv3.1 confers fast firing characteristics to neurones. Kv3.1b subunit immunoreactivity (Kv3.1b-IR) was widespread throughout the medulla oblongata, with labelled neurones in the gracile, cuneate and spinal trigeminal nuclei. In the nucleus of the solitary tract (NTS), Kv3.1b-IR neurones were predominantly located close to the tractus solitarius (TS) and could be GABAergic or glutamatergic. Ultrastructurally, Kv3.1b-IR was detected in NTS terminals, some of which were vagal afferents. Whole-cell current-clamp recordings from neurones near the TS revealed electrophysiological characteristics consistent with the presence of Kv3.1b subunits: short duration action potentials (4.2 ± 1.4 ms) and high firing frequencies (68.9 ± 5.3 Hz), both sensitive to application of TEA (0.5 mm) and 4-aminopyridine (4-AP; 30 μm). Intracellular dialysis of an anti-Kv3.1b antibody mimicked and occluded the effects of TEA and 4-AP in NTS and dorsal column nuclei neurones, but not in dorsal vagal nucleus or cerebellar Purkinje cells (which express other Kv3 subunits, but not Kv3.1b). Voltage-clamp recordings from outside-out patches from NTS neurones revealed an outward K+ current with the basic characteristics of that carried by Kv3 channels. In NTS neurones, electrical stimulation of the TS evoked EPSPs and IPSPs, and TEA and 4-AP increased the average amplitude and decreased the paired pulse ratio, consistent with a presynaptic site of action. Synaptic inputs evoked by stimulation of a region lacking Kv3.1b-IR neurones were not affected, correlating the presence of Kv3.1b in the TS with the pharmacological effects. PMID:15528247

  7. A polyether biotoxin binding site on the lipid-exposed face of the pore domain of Kv channels revealed by the marine toxin gambierol

    Science.gov (United States)

    Kopljar, Ivan; Labro, Alain J.; Cuypers, Eva; Johnson, Henry W. B.; Rainier, Jon D.; Tytgat, Jan; Snyders, Dirk J.

    2009-01-01

    Gambierol is a marine polycyclic ether toxin belonging to the group of ciguatera toxins. It does not activate voltage-gated sodium channels (VGSCs) but inhibits Kv1 potassium channels by an unknown mechanism. While testing whether Kv2, Kv3, and Kv4 channels also serve as targets, we found that Kv3.1 was inhibited with an IC50 of 1.2 ± 0.2 nM, whereas Kv2 and Kv4 channels were insensitive to 1 μM gambierol. Onset of block was similar from either side of the membrane, and gambierol did not compete with internal cavity blockers. The inhibition did not require channel opening and could not be reversed by strong depolarization. Using chimeric Kv3.1–Kv2.1 constructs, the toxin sensitivity was traced to S6, in which T427 was identified as a key determinant. In Kv3.1 homology models, T427 and other molecular determinants (L348, F351) reside in a space between S5 and S6 outside the permeation pathway. In conclusion, we propose that gambierol acts as a gating modifier that binds to the lipid-exposed surface of the pore domain, thereby stabilizing the closed state. This site may be the topological equivalent of the neurotoxin site 5 of VGSCs. Further elucidation of this previously undescribed binding site may explain why most ciguatoxins activate VGSCs, whereas others inhibit voltage-dependent potassium (Kv) channels. This previously undescribed Kv neurotoxin site may have wide implications not only for our understanding of channel function at the molecular level but for future development of drugs to alleviate ciguatera poisoning or to modulate electrical excitability in general. PMID:19482941

  8. Progress of the COST Action TU1402 on the Quantification of the Value of Structural Health Monitoring

    DEFF Research Database (Denmark)

    Thöns, Sebastian; Limongelli, Maria Pina; Ivankovic, Ana Mandic

    2017-01-01

    This paper summarizes the development of Value of Structural Health Monitoring (SHM) Information analyses and introduces the development, objectives and approaches of the COST Action TU1402 on this topic. SHM research and engineering has been focused on the extraction of loading, degradation...... for its quantification. This challenge can be met with Value of SHM Information analyses facilitating that the SHM contribution to substantial benefits for life safety, economy and beyond can be may be quantified, demonstrated and utilized. However, Value of SHM Information analyses involve complex models...... encompassing the infrastructure and the SHM systems, their functionality and thus require the interaction of several research disciplines. For progressing on these points, a scientific networking and dissemination project namely the COST Action TU1402 has been initiated....

  9. The 12th 735-kV transmission line project: Hydro-Quebec network

    International Nuclear Information System (INIS)

    1992-01-01

    Hydro-Quebec is proposing to construct a 735-kV transmission line from the Chissibi station near the LG3 hydroelectric power plant to the Jacques-Cartier station near Quebec City. The width of the right-of-way will generally be 90 m and it is intended to install the line in two stages. The Chissibi-Chibougamau section should enter service in 1993 and the Chibougamau-Cartier section in 1994. The cost of the line is estimated at $1.21 billion, of which $900 million is for line construction and $310 million for station equipment. Prospective corridors for the line route have been selected, portions of which enable twinning with Hydro-Quebec's existing 9th 735-kV line. A series of hearings on the environmental and other impacts of the power line project was held by the Bureau d'audiences publiques sur l'environnement and results are summarized along with the views of the Bureau. The summary describes the justification for the project, the choice of the transmission line route, the evaluation of the impacts of the project on the natural and human environments, health effects due to magnetic fields, and effects on the economic development of northern Quebec. Recommendations are given for mitigating the impacts of the project. 13 refs., 8 figs., 2 tabs

  10. Flatiron-Erie 115kV transmission line project, Larimer, Weld and Boulder Counties, Colorado

    International Nuclear Information System (INIS)

    1993-05-01

    Western Area Power Administration (Western) proposes to uprate its existing 115-kV Flatiron-Erie transmission line. The line is located in Larimer, Weld and Boulder Counties, Colorado, and passes through the City of Longmont. The line connects Flatiron Substation and several of the substations supplying Longmont. It is a single circuit 115-kV line, 31.5 miles long, and was built in 1950-51 on a 75-foot wide right-of-way (ROW) using wood H-frame structures. Western proposes to build 27 new structures along the line, to replace or modify 45 of the existing structures and to remote 11 of them. Many of these additions and changes would involve structures that are approximately 5 to 15 feet taller than the existing ones. The existing conductors and ground wires would remain in place. The purpose of these actions would be to allow the power carrying capability of the line to be increased and to replace deteriorating/structural members. Western would be the sole participant in the proposed project. This report gives an analysis of the study area environment and the development of alternative routes. An assessment is presented of the impacts of the primary alternative routes. The environmental consequences of this project are addressed

  11. An operational 150 kV microfocus rod anode X-ray system for nondestructive testing

    International Nuclear Information System (INIS)

    Fontijn, E.A.

    1978-01-01

    This paper describes an operational state of the art 150 kV microfocus rod anode X-ray system having ultra-high radiographic resolution capabilities. A cocal spot size of 0.050 mm is provided. Heretofore unattainable long rod anode lengths coupled with very small diameters are now possible using mini-magnetic lens technology. Over-all rod anode diameters as small as 9 mm with useful lengths of 1 m or more are possible, permitting panoramic inspections where previously only lower resolution radioisotope radiographic techniques were possible. Radiographic sensitivity of better than 1% has been reported with film-focal-distances on the order of 8 mm through 3 mm of steel. The system has been successfully applied to steam generator and heat exchanger tube-to-tubesheet weldments in both Europe and the USA. Other application areas include marine and aircraft jet engine inspection and numerous other applications where high reliability requirements indicate the use of a ultra-sensitive radiographic technique as is routinely demonstrated with the 150 kV Microfocus Rod Anode X-ray System. (orig.) [de

  12. 100 kV solid-state switch for fusion heating systems

    International Nuclear Information System (INIS)

    Beaumont, B.; Bertrand, E.; Brugnetti, R.; Chatroux, D.; Kazarian, F.; Milly, R.; Prou, M.; Rigole, H.

    2005-01-01

    Power switching in RF heating systems is a delicate function as it is often linked to high power tube protection. In most RF systems, the end stage power tube is fed by a high voltage power supply (HVPS), which connection to the tube has to be interrupted in case of arc suspicion. The amount of energy that is allowable to be dissipated in the arc is in the range of 10-50 J, to limit the degradations observed on the tube structures. The protection function is usually performed by a crowbar. Furthermore, the HVPS is often shared by several power tubes, and the loss of all the power from the group of tubes is to be avoided to minimize the perturbation on the plasma experiment. A description of a 40 kV thyristor based crowbar and a 100 kV, 25 A MOSFET switch is given, as well as the contours of the existing components for high power switching applications. By combining small components, such as thyristors or MOSFET, in matrix, highly compact and reliable units have been built and implemented in Tore Supra RF systems

  13. Trailing (L5) Neptune Trojans: 2004 KV18 and 2008 LC18

    International Nuclear Information System (INIS)

    Guan Pu; Zhou Liyong; Li Jian

    2012-01-01

    The population of Neptune Trojans is believed to be bigger than that of Jupiter Trojans and that of asteroids in the main belt, although only eight members of this distant asteroid swarm have been observed up to now. Six leading Neptune Trojans around the Lagrange point L 4 discovered earlier have been studied in detail, but two trailing ones found recently around the L 5 point, 2004 KV18 and 2008 LC18, have not yet been investigated. We report our investigations on the dynamical behaviors of these two new Neptune Trojans. Our calculations show that the asteroid 2004 KV18 is a temporary Neptune Trojan. Most probably, it was captured into the trailing Trojan cloud no earlier than 2.03 × 10 5 yr ago, and it will not maintain this position later than 1.65 × 10 5 yr in the future. Based on the statistics from our orbital simulations, we argue that this object is more like a scattered Kuiper belt object. By contrast, the orbit of 2008 LC18 is much more stable. Among the clone orbits spreading within the orbital uncertainties, a considerable portion of clones may survive on the L 5 tadpole orbits for 4 Gyr. The strong dependence of the stability on the semimajor axis and resonant angle suggests that further observations are badly required to constrain the orbit in the stable region. We also discuss the implications of the existence and dynamics of these two trailing Trojans over the history of the solar system.

  14. Engineering of beam direct conversion for a 120-kV, 1-MW ion beam

    International Nuclear Information System (INIS)

    Barr, W.L.; Doggett, J.N.; Hamilton, G.W.; Kinney, J.D.; Moir, R.W.

    1977-01-01

    Practical systems for beam direct conversion are required to recover the energy from ion beams at high efficiency and at very high beam power densities in the environment of a high-power, neutral-injection system. Such an experiment is now in progress using a 120-kV beam with a maximum total current of 20 A. After neutralization, the H + component to be recovered will have a power of approximately 1MW. A system testing these concepts has been designed and tested at 15 kV, 2 kW in preparation for the full-power tests. The engineering problems involved in the full-power tests affect electron suppression, gas pumping, voltage holding, diagnostics, and measurement conditions. Planning for future experiments at higher power includes the use of cryopumping and electron suppression by a magnetic field rather than by an electrostatic field. Beam direct conversion for large fusion experiments and reactors will save millions of dollars in the cost of power supplies and electricity and will dispose of the charged beam under conditions that may mot be possible by other techniques

  15. California-Oregon 500-kV transmission line development of design criteria

    International Nuclear Information System (INIS)

    Simpson, K.D.

    1990-01-01

    The California-Oregon Transmission Project (COTP) encompassed the design and construction of a third 500-kV ac intertie between California and the Pacific Northwest Transmission system. Sargent ampersand Lundy's (S ampersand L) scope of work in the COTP includes the design of approximately 150 miles of new single-circuit, 500-kV transmission line from southern Oregon to the vicinity of Redding, California. This paper presents the development of the design criteria for this segment of the project, which crosses diverse topographic and climatic regions. This project is an example of the increasing utilization of computers in transmission line engineering. Almost all aspects of design involved the use of the computer. Also, the development of the design criteria for this project coincided with an early release of the TLWorkstation software package by EPRI. TLWorkstation is an engineering workstation containing a family of programs for various aspects of transmission line design. This engineering software allows for increasing refinement in the design and economic optimization of transmission lines and is becoming an important design tool for transmission engineers

  16. Minimal exposure technique in the Cambridge University 600kV high resolution electron microscope

    International Nuclear Information System (INIS)

    Fryer, J.R.; Cleaver, J.R.A.; Smith, D.J.

    1980-01-01

    Radiation damage due to the incident electron beam imposes a fundamental limitation on the information obtainable by electron microscopy about organic materials; it is desirable therefore that exposure of the specimen to the electron beam should be restricted to the actual period during which the image is being recorded. A description is given of methods employed in the observation of the organic aromatic hydrocarbons quaterrylene, ovalene and coronene with the Cambridge University 600kV high resolution electron microscope (HREM). In particular, the condenser-objective mode of operation of this microscope lends itself to the use of an area-defining aperture below the second condenser lens conjugate with the specimen. Furthermore, operation at the higher accelerating voltage of this instrument could be anticipated to reduce the rate of damage, depending on the dominant beam-specimen interaction, whilst the increased width of the first broad band of the contrast transfer function of this microscope at the optimum defocus may overcome the reported resolution limitation of current 100kV microscopes for the observation of related materials. (author)

  17. Taurine activates delayed rectifier KV channels via a metabotropic pathway in retinal neurons

    Science.gov (United States)

    Bulley, Simon; Liu, Yufei; Ripps, Harris; Shen, Wen

    2013-01-01

    Taurine is one of the most abundant amino acids in the retina, throughout the CNS, and in heart and muscle cells. In keeping with its broad tissue distribution, taurine serves as a modulator of numerous basic processes, such as enzyme activity, cell development, myocardial function and cytoprotection. Despite this multitude of functional roles, the precise mechanism underlying taurine's actions has not yet been identified. In this study we report findings that indicate a novel role for taurine in the regulation of voltage-gated delayed rectifier potassium (KV) channels in retinal neurons by means of a metabotropic receptor pathway. The metabotropic taurine response was insensitive to the Cl− channel blockers, picrotoxin and strychnine, but it was inhibited by a specific serotonin 5-HT2A receptor antagonist, MDL11939. Moreover, we found that taurine enhanced KV channels via intracellular protein kinase C-mediated pathways. When 5-HT2A receptors were expressed in human embryonic kidney cells, taurine and AL34662, a non-specific 5-HT2 receptor activator, produced a similar regulation of KIR channels. In sum, this study provides new evidence that taurine activates a serotonin system, apparently via 5-HT2A receptors and related intracellular pathways. PMID:23045337

  18. Ingledow 500 kV substation : using mixed technology switchgear solutions for replacement

    Energy Technology Data Exchange (ETDEWEB)

    Wallner, C.; Glaubitz, P. [Siemens AG, Munich (Germany); Messinger, T. [British Columbia Transmission Corp., Vancouver, BC (Canada)

    2008-07-01

    The reliability of the 500 kV equipment at the Ingledow substation operated by BC Hydro Transmission System is of critical importance due to the energy interchange with the Bonneville Power Authority in the United States and the major role of the substation in serving the energy needs of British Columbia's densely populated Lower Mainland. Presently, the 500 kV switchyard includes 10 airblast circuit breakers and the associated current transformers which are all approaching their end of service-life. In response to environmental and safety concerns, the British Columbia Transmission Corporation adopted a policy whereby new oil-filled transformers are no longer acceptable, and the replacement of existing oil-filled transformers must be oil-free. The result is that the current transformers must be either bushing-type or sulphur-hexafluoride (SF6) type. The replacement strategies for the substation equipment consider economical, environmental and seismic issues. They have just as strong an influence on the decision process as the technical, operational and reliability aspects for the particular case or switchgear replacement in the transmission system. The Ingledow case demonstrates the advantages of using mixed technology switchgear, with its compact design flowing from gas insulated switchgear (GIS) for replacement of equipment in an outdoor switchyard. The advantages of the MTS in this case offer the possibility of future reconfiguration to improve the system reliability within the existing switchyard space. 3 refs., 5 figs.

  19. Engineering of beam direct conversion for a 120-kV, 1-MW ion beam

    International Nuclear Information System (INIS)

    Barr, W.L.; Doggett, J.N.; Hamilton, G.W.; Kinney, J.D.; Moir, R.W.

    1977-01-01

    Practical systems for beam direct conversion are required to recover the energy from ion beams at high efficiency and at very high beam power densities in the environment of a high-power, neutral-injection system. Such an experiment is now in progress using a 120-kV beam with a maximum total current of 20 A. After neutralization, the H + component to be recovered will have a power of approximately 1 MW. A system testing these concepts has been designed and tested at 15 kV, 2 kW in preparation for the full-power tests. The engineering problems involved in the full-power tests affect electron suppression, gas pumping, voltage holding, diagnostics, and measurement conditions. Planning for future experiments at higher power includes the use of cryopumping and electron suppression by a magnetic field rather than by an electrostatic field. Beam direct conversion for large fusion experiments and reactors will save millions of dollars in the cost of power supplies and electricity and will dispose of the charged beam under conditions that may not be possible by other techniques

  20. Image quality at low tube voltage (70 kV) and sinogram-affirmed iterative reconstruction for computed tomography in infants with congenital heart disease

    International Nuclear Information System (INIS)

    Nakagawa, Motoo; Ozawa, Yoshiyuki; Sakurai, Keita; Shimohira, Masashi; Shibamoto, Yuta; Ohashi, Kazuya; Asano, Miki; Yamaguchi, Sachiko

    2015-01-01

    Lower tube voltage has advantages for CT angiography, such as improved contrast To evaluate the image quality of low-voltage (70 kV) CT for congenital heart disease and the ability of sinogram-affirmed iterative reconstruction to improve image quality. Forty-six children with congenital heart disease (median age: 109 days) were examined using dual-source CT. Scans were performed at 80 kV and 70 kV in 21 and 25 children, respectively. A nonionic iodinated contrast medium (300 mg I/ml) was used for the 80-kV protocol. The contrast medium was diluted to 75% (225 mgI/mL) with saline for the 70-kV protocol. Image noise was measured in the two protocols for each group by extracting the standard deviations of a region of interest placed on the descending aorta. We then determined whether sinogram-affirmed iterative reconstruction reduced the image noise at 70 kV. There was more noise at 70 kV than at 80 kV (29 ± 12 vs 20 ± 4.8; P < 0.01). Sinogram-affirmed iterative reconstruction with grade 4 strength settings improved the noise (20 ± 5.9; P < 0.01) for the 70-kV group. Sinogram-affirmed iterative reconstruction improved the image quality of CT in congenital heart disease. (orig.)