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Sample records for trichostrongylus vitrinus nematoda

  1. Morphological and Morphometrical Description of Trichostrongylus Species Isolated from Domestic Ruminants in Khuzestan Province, Southwest Iran

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    B Golestan

    2011-09-01

    Full Text Available Backgrounds: Genus Trichostrongylus (Nematoda: Trichostrongylidae is one of the most impor­tant zoonotic nematodes with wide geographic distribution in the world. The purpose of the present study was to describe morphological and morphometrical characteristics of male Trichostrongylus species, currently prevalent in domestic ruminants of Khuzestan Province, south­west Iran.Methods: Gastro-intestinal organs of 1600 sheep, goats, cattle, and buffalos, slaughtered in Khuzestan Province, southwest Iran, were examined for infectivity with Trichostrongylus spe­cies. For examination and measurements of helminthes, Azo-carmine staining was per­formed, followed by camera lucida drawings of morphological characters and measurements of morphometrical criteria with a calibrated microscope. Using valid nematodes systematic keys, almost all the parasites were identified at the level of species.Results: Overall, 114 animals were found infected with at least one species of Trichostrongy­lus. Considering morphological characteristics of male Trichostrongylus, six species were identi­fied including T. colubriformis, T. vitrinus, T. probolorus, T. capricola, T. longispicu­laris and Trichostrongylus sp. Conclusion: Although, compared to the previous decades, currently Trichostrongylus is much less prevalent in the domestic ruminants of the study area, but still different species occur in these animals.

  2. Nematoda: Longidoridae

    African Journals Online (AJOL)

    1988-03-31

    Mar 31, 1988 ... Cytogenetics and reproduction in. Xiphinema and Longidorus. In: Nematode Vectors of. Plant Viruses, (eds.) Lamberti, F., Taylor, C.E. &. Seinhorst, I.W. Plenum Press, London and New York, pp. 139-151. DALMASSO, A. & YOUNES, T. 1969. Ovogenese et embryogenese chez Xiphinema index (Nematoda:.

  3. Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

    Czech Academy of Sciences Publication Activity Database

    Vadlejch, J.; Langrová, I.; Borovský, M.; Sedmíková, M.; Jankovská, I.; Fechtner, J.; Lytvynets, Andrej

    2006-01-01

    Roč. 43, č. 2 (2006), s. 64-68 ISSN 0440-6605 Institutional research plan: CEZ:AV0Z50110509 Keywords : Trichostrongylus colubriformis * hypobiosis * protein * enzymes * SDS-PAGE * API -ZYM Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.500, year: 2006

  4. Ivermectin: activity against larval Strongylus vulgaris and adult Trichostrongylus axei in experimental infections in ponies.

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    Lyons, E T; Drudge, J H; Tolliver, S C

    1982-08-01

    Activity of ivermectin, administered IM at the dosage rate of 200 micrograms/kg of body weight, was evaluated in controlled tests against migrating larvae of Strongylus vulgaris and adult Trichostrongylus axei in experimental infections in 6 ponies raised worm-free. Ponies were given 2,190 or 2,400 infective 3rd-stage larvae of S vulgaris at 7 days before treatment and 22,000 or 22,750 infective 3rd-stage larvae of T axei at 42 or 45 days before treatment. Three ponies were given ivermectin plus vehicle, and 3 ponies were given the vehicle only; the ponies were euthanatized 7 or 9 days after treatment. At necropsy, 4th-stage S vulgaris larvae were not recovered from visceral arteries of the 3 ivermectin plus vehicle-treated ponies, but 21 to 40 larvae were recovered from each of the 3 vehicle-treated ponies. Also at necropsy, adult T axei (140 specimens) were recovered from only 1 ot the 3 ivermectin plus vehicle-treated ponies, but 4,610 to 6,410 specimens were found in each of the 3 vehicle-treated ponies. Toxicosis was not observed after treatment.

  5. The role of thresholds in the response of lambs to vaccination with irradiated Trichostrongylus colubriformis larvae

    International Nuclear Information System (INIS)

    Windon, R.G.; Dineen, J.K.; Gregg, P.; Donald, A.D.; Griffiths, D.A.

    1984-01-01

    A piecewise logarithmic model fitted to worm counts of ewe lambs vaccinated and challenged in pens with a range of doses of irradiated and normal Trichostrongylus colubriformis larvae respectively, indicated that the threshold for response to both vaccine (V 0 =4400) and challenge dose is exceeded by 5000 larvae. Whereas response was vaccine dose dependent, it was independent of challenge dose. Ram lambs vaccinated at low dose levels were as resistant against challenge as ewe lambs, but by contrast, failed to show increased protection after vaccination with high doses of irradiated larvae. Serum titre of antiworm complement-fixing antibodies at the time of challenge also indicated that ram lambs were less responsive immunologically than ewe lambs following vaccination at the higher dose levels. A field study showed that response to vaccination was only apparent after transfer of the sheep to heavily contaminated pastures, suggesting that previous exposure of the vaccinated animals to the low dose of infective larvae available on a lightly contaminated pasture constituted a challenge which was below the threshold. (author)

  6. Effects of dietary protein intake on responses of young sheep to infection with Trichostrongylus colubriformis.

    Science.gov (United States)

    van Houtert, M F; Barger, I A; Steel, J W; Windon, R G; Emery, D L

    1995-01-01

    The effects of protein supplementation and infection with Trichostrongylus colubriformis on production responses and immune function in young sheep and on nematode population dynamics were assessed. Eighty-four 3-month-old Merino wether sheep were housed in individual pens and fed ad libitum chopped oaten hay containing 0.5% urea, together with 50 g day-1 lucerne meal. Fish meal (FM) was given as a source of protected protein at 0, 50 or 100 g day-1 (FM0, FM50, FM100; from Days --28 to 140). From Days 1 to 140, 0 or 1000 T. colubriformis infective larvae were given on Mondays, Wednesdays and Fridays. Infected sheep were slaughtered after 35, 70, 105, or 140 days of infection. Live-weight gain was reduced significantly by infection with T. colubriformis in sheep given FM0, but not in sheep given FM50 or FM100. Greasy wool production and fibre diameter were increased by FM, whereas the effects of infection with T. colubriformis on wool measurements depended on the level of FM given. Worm egg concentrations in faeces were significantly lower for sheep given FM100 than for those given FM0 or FM50 during the last 28 days of infection. Similarly, the apparent rate of worm expulsion was considerably higher in sheep given FM than in those not given FM. The rate of expulsion of T. colubriformis correlated with levels of circulating eosinophils as well as with the concentration of intestinal sheep mast cell proteases. Levels of parasite-specific and non-specific circulating antibodies were either unaffected or reduced as a result of supplementation with FM, although lymphocyte stimulation in vitro in response to T. colubriformis third stage larval antigen was enhanced significantly in infected animals given FM100. It was concluded that supplementary feeding with FM substantially reduced the production losses attributable to infection with T. colubriformis and was associated with enhanced expulsion of the parasite burden.

  7. First record of Mermithidae (Nematoda) parasitic in Plecoptera

    OpenAIRE

    Ravizza, C.; Zwick, P.

    2006-01-01

    The first record of Nematoda Mermithidae in adults and larvae of two Protonemura species (Plecoptera Nemouridae) and of Isoperla rivulorum (Pictet) (Plecoptera Perlodidae) from North Italy and Bavaria (Germany) is presented. The malformed genitalia of several parasitically castrated adults are described and illustrated. Primi reperti di Mermithidae (Nematoda) endoparassiti di Plecoptera. Viene segnalata per la prima volta la presenza di Mermithidae (Nematoda) nell’addome di adulti e di ninfe ...

  8. Dried, ground banana plant leaves (Musa spp.) for the control of Haemonchus contortus and Trichostrongylus colubriformis infections in sheep.

    Science.gov (United States)

    Gregory, L; Yoshihara, E; Ribeiro, B L M; Silva, L K F; Marques, E C; Meira, E B S; Rossi, R S; Sampaio, P H; Louvandini, H; Hasegawa, M Y

    2015-12-01

    To evaluate the anthelmintic effect of Musa spp. leaves, 12 animals were artificially infected with Haemonchus contortus, and another 12 animals were infected with Trichostrongylus colubriformis. Then, both treatment groups were offered 400 g of dried ground banana plant leaves, and the control animals were offered only 1000 g of coast cross hay. During the trials, the animals received weekly physical examinations. The methods used to evaluate the efficiency of this treatment were packed cell volume, total plasma protein and faecal egg counts, and egg hatchability tests were performed on days -2, +3, +6, +9, +13 and +15. Coproculture tests were performed on day -2 to confirm monospecific infections. In the FEC and EHT, a statistically significant difference (0.04, 0.005; p  0.05) for Haemochus contortus group in all tests. Our results confirmed previous findings suggesting that dried ground banana plant leaves possess anthelmintic activity.

  9. Effects of size of Trichostrongylus colubriformis infections on histopathology of the mucosa along the whole small intestine in rabbits.

    Science.gov (United States)

    Hoste, H; Mallet, S

    1990-11-01

    The influence of population size of Trichostrongylus colubriformis on the structures of the small intestine, especially with regard to the development and origin of an intestinal adaptive response, was examined in experimentally infected rabbits. The effects of low (500 L3) and high (50,000 L3) infection on histological (villous length, mucosa to serosa ratio, crypt surface) and biochemical (protein content, alkaline phosphatase and leucine aminopeptidase activities) aspects of the mucosa were assessed along the whole small intestine. The presence of a small number of worms induced only minor mucosal changes, indicating a regenerative response of the intestinal epithelium. The role of a local small population of T. colubriformis in the development of a previously described adaptive response appeared thus to be limited. On the other hand, the 50,000 L3 inoculum was associated with severe lesions of villi, marked crypt hyperplasia and with a major reduction of enzyme activities. The changes were found along the whole length of the small intestine. These results suggest that the generally recognized dose-dependent pathogenicity of the intestinal nematode infections could be ascribed to two different processes: firstly, a greater severity of the lesions; secondly, more extensive damage leading to the disappearance of any adaptive intestinal region.

  10. Anthelmintic efficacy of five tropical native Australian plants against Haemonchus contortus and Trichostrongylus colubriformis in experimentally infected goats (Capra hircus).

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    Moreno, F C; Gordon, I J; Knox, M R; Summer, P M; Skerrat, L F; Benvenutti, M A; Saumell, C A

    2012-06-08

    The study of the anthelmintic properties of plants rich in plant secondary metabolites can provide ecologically sound methods for the treatment of parasites on grazing animals. The purpose of the present study was to evaluate the anthelmintic effect of five tropical native Australian plant species rich in plant secondary metabolites on adult Haemonchus contortus and Trichostrongylus colubriformis in experimentally infected goats. Thirty young, nematode-free goats were infected with 2500 H. contortus and 5000 T. colubriformis infective larvae thrice weekly for a week (day 1-7 of the experiment). On day 27 after first infection, the goats were allocated into six groups of five animals per group. From day 28 to day 35, fresh leaves from Acacia salicina, Acacia nilotica, Eucalyptus corymbia, Casuarina cunninghamiana and Eucalyptus drepanophylla were included in the goats diet. Five groups were offered leaves from one of these plant species and one group, the untreated control, received only the basal diet formulated with 20% Medicago sativa and 80% Avena sativa. Following plant material administration, the goats were monitored daily until day 40 and then slaughtered on day 41. Total faecal worm egg output, total production of larvae recovered from faecal cultures, total post-mortem worm burdens and the per capita fecundity of female worms were estimated. The toxicity of the plant species for the goats was measured by histopathological analyses of liver and kidney samples. Results showed that goats feeding on the plant material rich in plant secondary metabolites had significantly lower egg output compared to the control goats (P0.05), the per capita fecundity was significantly reduced by E. corymbia, A. nilotica and A. salicina (Pgoats can benefit from the short-term ingestion of plant secondary metabolites, which reduce the total faecal egg output and thus decrease the potential for re-infection from the pasture. Crown Copyright © 2012. Published by Elsevier B.V. All

  11. ANTHELMINTIC EFFECTS OF DRIED GROUND BANANA PLANT LEAVES (MUSA SPP.) FED TO SHEEP ARTIFICIALLY INFECTED WITH HAEMONCHUS CONTORTUS AND TRICHOSTRONGYLUS COLUBRIFORMIS.

    Science.gov (United States)

    Gregory, Lilian; Yoshihara, Eidi; Silva, Leandro Kataoaka Fernandes; Marques, Eduardo Carvalho; Ribeiro, Bruno Leonardo Mendonça; de Souza Meira, Enoch Brandão; Rossi, Rodolfo Santos; do Amarante, Alessandro Francisco Talamini; Hasegawa, Marjorie Yumi

    2017-01-01

    Helminths is a endoparasites that cause the major losses for profitable sheep production in Brazil. The increased development of resistant strains of endoparasites have enforced the search for sustainable alternatives. The aim of this paper was to provide information about endoparasites control with banana leaves in infected sheep as alternative control strategies and see its viability. In this study, we performed two trials to investigate the anthelmintic properties of banana leaves on endoparasites in sheep. In Trial 1, twelve sheep were artificially infected with Trichostrongylus colubriformis ; in Trial 2, eleven sheep were artificially infected with Haemonchus contortus . Clinical examinations, packed cell volume, total protein, faecal egg counts (FECs) and egg hatchability tests (EHTs) were performed. At the end of the trials, the sheep were humanely slaughtered, and total worm counts were performed. In Trial 1 and 2, no significant FEC decreases were note but significant diference in EHTs were observed. Total worm counts, clinical and haematological parameters did not reveal significant changes between the treatment and control groups. These results suggest that feeding dried ground banana plant leaves to sheep may reduce the viability of Trichostrongylus colubriformis eggs, and this anthelmintic activity is potentially exploitable as part of an integrated parasite management programme. However, further investigation is needed to establish the optimal dosage, develop a convenient delivery form and confirm the economic feasibility of using banana plantation byproducts as feed for ruminant species. Abbreviations: Coproculture test (CT)., Faecal egg count (FEC)., Egg hatchability test (EHT).

  12. New records of Ascaridia platyceri (Nematoda) in parrots (Psittaciformes)

    Czech Academy of Sciences Publication Activity Database

    Kajerová, V.; Baruš, Vlastimil; Literák, I.

    2004-01-01

    Roč. 49, č. 7 (2004), s. 237-241 ISSN 0375-8427 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z6093917 Keywords : ascarids * morphology * Nematoda Subject RIV: EG - Zoology Impact factor: 0.790, year: 2004 http://www.vri.cz/docs/vetmed/49-7-237.pdf

  13. Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis.

    Science.gov (United States)

    Arroyo-Lopez, Celia; Manolaraki, Foteini; Saratsis, Anastasios; Saratsi, Katerina; Stefanakis, Alexandros; Skampardonis, Vasileios; Voutzourakis, Nikolaos; Hoste, Hervé; Sotiraki, Smaragda

    2014-01-01

    The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia) and carob pod meal (Ceratonia siliqua) against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay and group CAR was fed with carob pods. The remaining lambs received lucerne hay (Medicago sativa) and were assigned to positive (non-treated, NT) and negative (treated, T) control groups (treatment with albendazole). On day 0, lambs were artificially trickle infected for 6 weeks, with a mixture of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis. Parasitological and pathophysiological parameters were measured repeatedly during the 2-month study. Compared to the NT group, decreases in egg excretion were observed in the CAR and S groups with significant differences only found for sainfoin (p < 0.05). At necropsy, group S showed decreases in the total worm numbers of both nematode species with significant differences for H. contortus. In contrast, no differences were noticed for the CAR group. Compared to the NT group, lower values for fecundity of female H. contortus were found in the S and CAR groups, however differences were non-significant. No differences in body weight gains were found between groups. Consistent results were found showing significantly higher packed cell volume (PCV) values in the T and S groups compared to NT and CAR groups. Overall, these results confirm a positive effect associated with the feeding of lambs with tanniniferous resources on host resilience (PCV values) and against gastrointestinal parasitic nematodes by affecting some biological traits of worm populations (e.g. eggs per gram of faeces and worm numbers). However, the anthelmintic effects differed between the two tannin-containing resources, which might be associated with the quantity and/or quality of secondary metabolites (condensed tannins and/or other

  14. Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis

    Directory of Open Access Journals (Sweden)

    Arroyo-Lopez Celia

    2014-01-01

    Full Text Available The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia and carob pod meal (Ceratonia siliqua against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay and group CAR was fed with carob pods. The remaining lambs received lucerne hay (Medicago sativa and were assigned to positive (non-treated, NT and negative (treated, T control groups (treatment with albendazole. On day 0, lambs were artificially trickle infected for 6 weeks, with a mixture of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis. Parasitological and pathophysiological parameters were measured repeatedly during the 2-month study. Compared to the NT group, decreases in egg excretion were observed in the CAR and S groups with significant differences only found for sainfoin (p < 0.05. At necropsy, group S showed decreases in the total worm numbers of both nematode species with significant differences for H. contortus. In contrast, no differences were noticed for the CAR group. Compared to the NT group, lower values for fecundity of female H. contortus were found in the S and CAR groups, however differences were non-significant. No differences in body weight gains were found between groups. Consistent results were found showing significantly higher packed cell volume (PCV values in the T and S groups compared to NT and CAR groups. Overall, these results confirm a positive effect associated with the feeding of lambs with tanniniferous resources on host resilience (PCV values and against gastrointestinal parasitic nematodes by affecting some biological traits of worm populations (e.g. eggs per gram of faeces and worm numbers. However, the anthelmintic effects differed between the two tannin-containing resources, which might be associated with the quantity and/or quality of secondary metabolites (condensed tannins and

  15. Relationship of intestinal histology with the resistance to Trichostrongylus colubriformis infection in three breeds of sheep Associação entre a resposta celular no intestino e a resistência à infecção por Trichostrongylus colubriformis em três raças de ovinos

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    Alessandro F.T. Amarante

    2007-01-01

    Full Text Available The study was carried out to evaluate the relationship of inflammatory intestinal cells with the resistance to Trichostrongylus colubriformis infections in three breeds of sheep (Santa Ines, Suffolk and Ile de France, naturally infected. Mast cells, eosinophils, and globule leucocytes were enumerated in intestinal mucosa. Histamine concentration was estimated in intestinal tissue samples and the length of male and female specimens were determined. The three breeds of sheep showed similar cellular response in the small intestine mucosa (P>0.05. There was extensive variation among sheep in the parasitological and inflammatory cell variables, even in lambs of the same breed. In general, animals presenting less inflammatory cells had a larger worm burden, higher fecal egg counts, and larger T. colubriformis worms. The inflammatory cells possibly impaired the parasite's establishment, development, and survival.Avaliaram-se a associação entre o número de células inflamatórias no intestino delgado e a resistência à infecção por Trichostrongylus colubriformis em ovinos de três raças (Santa Inês, Suffolk e Ile de France, naturalmente infectados. Mastócitos, eosinófilos e leucócitos globulares foram quantificados na mucosa intestinal. A concentração de histamina foi estimada em amostras teciduais do intestino, bem como foi determinado o comprimento de machos e fêmeas de T. colubriformis. A resposta celular foi similar na mucosa intestinal das três raças ovinas (P>0,05. Houve grande variação entre os ovinos em relação aos resultados parasitológicos e celulares, mesmo nos animais de mesma raça. Em geral, os animais que apresentaram número menor de células inflamatórias tiveram cargas parasitárias maiores, contagens de ovos por grama de fezes mais altas e exemplares de T. colubriformis maiores. Os resultados indicaram que mastócitos, eosinófilos e leucócitos globulares prejudicaram o estabelecimento, o desenvolvimento e a

  16. Evolution of plant parasitism in the phylum Nematoda.

    Science.gov (United States)

    Quist, Casper W; Smant, Geert; Helder, Johannes

    2015-01-01

    Within the species-rich and trophically diverse phylum Nematoda, at least four independent major lineages of plant parasites have evolved, and in at least one of these major lineages plant parasitism arose independently multiple times. Ribosomal DNA data, sequence information from nematode-produced, plant cell wall-modifying enzymes, and the morphology and origin of the style(t), a protrusible piercing device used to penetrate the plant cell wall, all suggest that facultative and obligate plant parasites originate from fungivorous ancestors. Data on the nature and diversification of plant cell wall-modifying enzymes point at multiple horizontal gene transfer events from soil bacteria to bacterivorous nematodes resulting in several distinct lineages of fungal or oomycete-feeding nematodes. Ribosomal DNA frameworks with sequence data from more than 2,700 nematode taxa combined with detailed morphological information allow for explicit hypotheses on the origin of agronomically important plant parasites, such as root-knot, cyst, and lesion nematodes.

  17. Potensi Jamur Parasit Telur Sebagai Agens Hayati Pengendali Nematoda Puru Akar Meloidogyne incognita pada Tanaman Tomat

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    Siwi Indarti

    2014-12-01

    Full Text Available Root-knot nematodes Meloidogyne spp. are sedentary endoparasitic that attacks various economically important plants. Utilization of nematode’s fungal egg parasite as biocontrol agents of sedentary endoparasitic nematodes have a good possibility of potential success to be applied in the field level, because this fungi is able to colonize in and causes damage to eggs as well as female nematodes inside the root. The purpose of this research are to know the parasitism ability of this parasitic fungi to Meloidogyne incognita eggs, and its effects on second stage larvae hatching rate and the development of galls number in the host. The result shows that the parasitic fungi, those of Trichoderma, Penicillium, Talaromyces, Fusarium genera were able to parasitize root-knot nematode eggs (25.09 to 89.79%, caused root-knot nematode egg hatching to decrease, suppressed the formation of galls, and reduced the population of second stage nematode larvae in the greenhouse. Nematoda puru-akar Meloidogyne spp. adalah nematoda endoparasitik sedentari, bersifat polifag, dan mempunyai nilai ekonomi tinggi. Pemanfaatan jamur parasit telur sebagai agens hayati pengendali nematoda endoparasitik sedentari mempunyai potensi tingkat keberhasilan tinggi untuk diterapkan pada aras lapangan karena mampu mengoloni dan merusak telur maupun stadium nematoda betina yang terlindungi jaringan tanaman. Tujuan penelitian adalah untuk mengetahui kemampuan parasitasi isolat-isolat jamur parasit telur terhadap telur nematoda Meloidogyne incognita, dan pengaruhnya terhadap tingkat penetasan telur menjadi L-2, serta pembentukan jumlah puru pada tanaman terserang. Hasil penelitian didapatkan bahwa jamur parasit telur yang termasuk genera Tricoderma, Penicillium, Talaromyces, dan Fusarium mampu memarasit telur M. incognita berkisar antara 25,09–89,79%, mengakibatkan penurunan persentase jumlah L-2 nematoda yang bersangkutan, serta menekan pembentukan puru akar pada aplikasi aras

  18. [Distribution of nematode parasites of the digestive system in sheep (Ovis aries) and goats (Capra hircus) of the Piedmontese and Valdostano Alpine arc].

    Science.gov (United States)

    Balbo, T; Costantini, R; Gallo, M G; Lanfranchi, P

    1977-01-01

    A survey, carried out on gastro-intestinal nematodes of sheep and goats of Piemonte and of Valle d'Aosta (87 sheep and 12 goats) has shown the presence of the following species in sheep, Bunostomum trigonocephalum, Chabertia ovina, Cooperia curticei, Haemonchus contortus, Marshallagia marshalli, Nematodirus abnormalis, Nematodirus filicollis, Nematodirus helvetianus, Nematodirus spathiger, Oesophagostomum venulosum, Ostertagia circumcincta, Ostertagia lyrata, Ostertagia trifurcata, Skrjabinema ovis, Trichostrongylus axei, Trichostronglus colubriformis, Trichostronglus vitrinus, Trichuris ovis and Trichuris skrjabini; in goats, Bunostomum trigonocephalum, Chabertia ovina, Haemonchus contortus, Nematodirus filicollis, Nematodirus helvetianus, Oesophagostomum venulosum, Ostertagia circumcincta, Ostertagia ostertagi, Ostertagia trifurcata, Trichostrongylus axei, Trichostrongylus colubriformis and Trichostrongylus vitrinus. The percentage of each species in the two host is given in the text table.

  19. Teaching Population Growth Using Cultures of Vinegar Eels, "Turbatrix aceti" (Nematoda)

    Science.gov (United States)

    Wallace, Robert L.

    2005-01-01

    A simple laboratory exercise is presented that follows the population growth of the common vinegar eel, "Turbatrix aceti" (Nematoda), in a microcosm using a simple culture medium. It lends itself to an exercise in a single semester course. (Contains 4 figures.)

  20. Observations on the distribution and biology of Huffmanela huffmani (Nematoda: Trichosomoididae)

    Czech Academy of Sciences Publication Activity Database

    Cox, M. K.; Huffman, D. G.; Moravec, František

    2004-01-01

    Roč. 51, č. 1 (2004), s. 50-54 ISSN 0015-5683 R&D Projects: GA AV ČR IAA6022201 Institutional research plan: CEZ:AV0Z6022909 Keywords : Nematoda * Trichosomoididae * Huffmanela Subject RIV: EA - Cell Biology Impact factor: 0.837, year: 2004

  1. Fauna of soil nematodes (Nematoda) in coal post-mining sites in Illinois, USA

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2013-01-01

    Roč. 77, č. 2 (2013), s. 103-112 ISSN 1211-376X R&D Projects: GA MŠk ME08019 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : soil zoology * ecology * Nematoda * species and generic richness * faunal similarity Subject RIV: EH - Ecology, Behaviour

  2. Morphometric and molecular characteristics of Labeonema synodontisi n. comb. (Nematoda: Atractidae) from the West African fishes

    Czech Academy of Sciences Publication Activity Database

    Koubková, B.; Baruš, Vlastimil; Hodová, I.; Šimková, A.

    2008-01-01

    Roč. 102, č. 5 (2008), s. 1013-1020 ISSN 0932-0113 R&D Projects: GA AV ČR IAA6093404; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60930519 Keywords : Nematoda * West African fishes * genetics Subject RIV: EG - Zoology Impact factor: 1.473, year: 2008

  3. Ultrastructure of the body wall of female Philometra obturans (Nematoda: Dracunculoidea)

    Czech Academy of Sciences Publication Activity Database

    Frantová, Denisa; Bruňanská, Magdaléna; Fagerholm, H.-P.; Kihlström, M.

    2005-01-01

    Roč. 95, č. 5 (2005), s. 327-332 ISSN 0932-0113 R&D Projects: GA ČR(CZ) GA524/03/0061 Institutional research plan: CEZ:AV0Z60220518 Keywords : Nematoda * ultrastructure * cuticule Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.226, year: 2005

  4. Morphometric and molecular characterization of Parapharyngodon echinatus (Nematoda, Pharyngodonidae) from the Senegal gecko ( Tarentola parvicarinata )

    Czech Academy of Sciences Publication Activity Database

    Mašová, Š.; Baruš, Vlastimil; Hodová, I.; Matějusová, I.; Koubek, Petr; Koubková, B.

    2008-01-01

    Roč. 53, č. 3 (2008), s. 274-283 ISSN 1230-2821 R&D Projects: GA AV ČR IAA6093404 Institutional research plan: CEZ:AV0Z60930519 Keywords : Nematoda * Senegal gecko * West Africa Subject RIV: EG - Zoology Impact factor: 0.748, year: 2008

  5. Agamermis (Nematoda: Mermithidae) Infection in South Carolina Agricultural Pests.

    Science.gov (United States)

    Stubbins, Francesca L; Agudelo, Paula; Reay-Jones, Francis P F; Greene, Jeremy K

    2016-12-01

    Native and invasive stink bugs (Hemiptera: Pentatomidae) and the closely related invasive Megacopta cribraria (Hemiptera: Plataspidae) are agricultural pests in the southeastern United States. Natural enemies, from various phyla, parasitize these pests and contribute to population regulation. We specifically investigated Nematoda infections in pentatomid and plataspid pests in one soybean field in South Carolina in 2015. Nematodes were identified through molecular and morphological methods and assigned to family Mermithidae, genus Agamermis . This study reports mermithid nematode infection in immature M. cribraria for the first time and provides the first mermithid host record for the stink bugs Chinavia hilaris , Euschistus servus , and another Euschistus species, and a grasshopper (Orthoptera: Acrididae) in South Carolina. The same Agamermis species infected all hosts. The broad host range and prevalence suggests that Agamermis may be an important contributor to natural mortality of pentatomid and plataspid pests. Previous mermithid host records for the Pentatomidae and Plataspidae worldwide are summarized. Further work is needed to assess the impact of infection on populations over a broader range of agricultural fields and geographic localities.

  6. Mitochondrial genome diversity in dagger and needle nematodes (Nematoda: Longidoridae).

    Science.gov (United States)

    Palomares-Rius, J E; Cantalapiedra-Navarrete, C; Archidona-Yuste, A; Blok, V C; Castillo, P

    2017-02-02

    Dagger and needle nematodes included in the family Longidoridae (viz. Longidorus, Paralongidorus, and Xiphinema) are highly polyphagous plant-parasitic nematodes in wild and cultivated plants and some of them are plant-virus vectors (nepovirus). The mitochondrial (mt) genomes of the dagger and needle nematodes, Xiphinema rivesi, Xiphinema pachtaicum, Longidorus vineacola and Paralongidorus litoralis were sequenced in this study. The four circular mt genomes have an estimated size of 12.6, 12.5, 13.5 and 12.7 kb, respectively. Up to date, the mt genome of X. pachtaicum is the smallest genome found in Nematoda. The four mt genomes contain 12 protein-coding genes (viz. cox1-3, nad1-6, nad4L, atp6 and cob) and two ribosomal RNA genes (rrnL and rrnS), but the atp8 gene was not detected. These mt genomes showed a gene arrangement very different within the Longidoridae species sequenced, with the exception of very closely related species (X. americanum and X. rivesi). The sizes of non-coding regions in the Longidoridae nematodes were very small and were present in a few places in the mt genome. Phylogenetic analysis of all coding genes showed a closer relationship between Longidorus and Paralongidorus and different phylogenetic possibilities for the three Xiphinema species.

  7. Species of Angiostrongylus (Nematoda: Metastrongyloidea in wildlife: A review

    Directory of Open Access Journals (Sweden)

    David M. Spratt

    2015-08-01

    Full Text Available Twenty-one species of Angiostrongylus plus Angiostrongylus sp. (Nematoda: Metastrongyloidea are known currently in wildlife. These occur naturally in rodents, tupaiids, mephitids, mustelids, procyonids, felids, and canids, and aberrantly in a range of avian, marsupial and eutherian hosts including humans. Adults inhabit the pulmonary arteries and right atrium, ventricle and vena cava, bronchioles of the lung or arteries of the caecum and mesentery. All species pass first-stage larvae in the faeces of the host and all utilise slugs and/or aquatic or terrestrial snails as intermediate hosts. Gastropods are infected by ingestion or penetration of first-stage larvae; definitive hosts by ingestion of gastropods or gastropod slime. Transmission of at least one species may involve ingestion of paratenic hosts. Five developmental pathways are identified in these life cycles. Thirteen species, including Angiostrongylus sp., are known primarily from the original descriptions suggesting limited geographic distributions. The remaining species are widespread either globally or regionally, and are continuing to spread. Small experimental doses of infective larvae (ca. 20 given to normal or aberrant hosts are tolerated, although generally eliciting a granulomatous histopathological response; large doses (100–500 larvae often result in clinical signs and/or death. Two species, A. cantonensis and A. costaricensis, are established zoonoses causing neurological and abdominal angiostrongliasis respectively. The zoonotic potential of A. mackerrasae, A. malaysiensis and A. siamensis particularly warrant investigation. Angiostrongylus cantonensis occurs in domestic animals, mammalian and avian wildlife and humans in the metropolitan areas of Brisbane and Sydney, Australia, where it has been suggested that tawny frogmouths and brushtail possums may serve as biosentinels. A major conservation issue is the devastating role A. cantonensis may play around zoos and fauna

  8. Redescription of Cucullanus robustus (Nematoda: Cucullanidae) from the conger eel Conger myriaster off Korea

    Czech Academy of Sciences Publication Activity Database

    Park, J. K.; Moravec, František

    2008-01-01

    Roč. 1729, - (2008), s. 1-7 ISSN 1175-5326 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Grant - others: Korea Research Foundation(KR) KRF-2006-C00544 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cucullanus * Cucullanidae * Conger * fish parasite * Korea * Nematoda Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.740, year: 2008

  9. Mercury content in Anguillicola crassus (Nematoda) and its host Anguilla anguilla

    Czech Academy of Sciences Publication Activity Database

    Palíková, M.; Baruš, Vlastimil

    2003-01-01

    Roč. 72, č. 2 (2003), s. 289-294 ISSN 0001-7213 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z6093917 Keywords : Nematoda * parasites * Anguilla anguilla Subject RIV: EG - Zoology Impact factor: 0.336, year: 2003 http://vfu-www.vfu.cz/acta-vet/vol72/pdf/72_289.pdf

  10. Philometra ovata (Nematoda: Philometroidea): a potential sentinel species of heavy metal accumulation

    Czech Academy of Sciences Publication Activity Database

    Baruš, Vlastimil; Jarkovský, J.; Prokeš, Miroslav

    2007-01-01

    Roč. 100, č. 5 (2007), s. 929-933 ISSN 0932-0113 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MZe(CZ) QF3029 Institutional research plan: CEZ:AV0Z60930519; CEZ:AV0Z60220518 Keywords : Nematoda * heavy metal accumulation Subject RIV: EG - Zoology Impact factor: 1.512, year: 2007

  11. IDENTIFIKASI NEMATODA PARASIT PADA SALURAN PENCERNAAN MARMUT (Cavia cobaya SEBAGAI SUMBER BELAJAR BIOLOGI PADA MATERI INVERTEBRATA

    Directory of Open Access Journals (Sweden)

    Septia Nurhasanah

    2014-05-01

    Full Text Available The research was conducted in the Laboratory of Science Education, University of Muhammadiyah Metro. The research is a descriptive analysis, morphological observations were made by identifying the nematode parasite found that Trichostrongylus sp, Strongyloides sp, Trichuris sp, Trichuris sp. The results showed the prevalence and intensity values is the large number of guinea pigs to parasites divided by the number of guinea pigs that are 100% inspected. While the intensity is seen from the total number of nematode parasites that infect in the number of guinea pigs infected with a parasite that is 2,3. The population in this study was 10 cows pigs (Cavia cobaya . This study took a part in the digestion of marmots (Cavia cobaya the intestines and stomach , then cut and taken part in the large intestine and be Indian ink or coloring 2-3 drops to be easily identified microscopically . Put the preparations into a petri dish , and given alcohol as much as 96 % with 2-3 drops . Taking part in the examination of the large intestine to taste then used the nematode worm structure with Indian ink dye to be observed , and placed in the glass object . Observed with a microscope. Document if the parasites are found by using the camera Erlina 2008. Identification of the observations made have found 3 types of parasites that attack the guinea pig (Cavia cobaya is Strongyloides sp on samples 1,2,3,4,5,6 , dan10 . Then parasite Trichuris sp species found in samples 8 and 9. Trichostrongylus sp parasite species found in sample 7 . Parasitic nematodes found were 3 types of Trichostrongylus sp, Strongyloides sp, Trichuris sp. Number of samples that have been observed as many as 10 cows pigs (Cavia cobaya , with the overall result is as much as 23 tails parasites.               Kata kunci: nematode parasit, marmut (Cavia cobaya, sumber belajar biologi.

  12. TTrichostrongylus retortaeformis (Zeder, 1800 (Nematoda, Trichostrongyloidea in Lepus europaeus (Pallas, 1778 in southern Brazil

    Directory of Open Access Journals (Sweden)

    Laura Maria Farias dos Santos

    2016-10-01

    Full Text Available The aim of this study was to evaluate the presence of helminthes parasitizing hares (Lepus europaeus in southern Brazil. The intestinal tracts of seven hares were opened and the contents were sieved. Among the seven animals in the study, Trichostrongylus retortaeformis parasitized six (85.7%. This study will be expanded and more animals captured to evaluate the occurrence of other helminthes and to assess whether the high prevalence of T. retortaeformis is accurate, as well as to assess the abundance and intensity of parasites. To our knowledge, this is the first record of T.retortaeformis parasitizing L. europaeus in southern Brazil.

  13. Anguillicola crassus (Nematoda, Dracunculoidea) infections of European eel (Anguila anguilla) in the Netherlands : epidemiology, pathogenensis and pathobiology

    NARCIS (Netherlands)

    Haenen, O.L.M.

    1995-01-01


    In the 1980s an eel parasitic nematode, Anguillicola crassus (Nematoda, Dracunculoidea), which infects the swimbladder of European eels ( Anguilla anguilla ) and other freshwater fish species, was introduced into The Netherlands. This thesis

  14. Ultrastructure of the body wall of Cystidicoloides ephemeridarum (Nematoda, Cystidicolidae) in relation to the histopathology of this nematode in salmonids

    Czech Academy of Sciences Publication Activity Database

    Frantová, Denisa; Moravec, František

    2003-01-01

    Roč. 91, č. 2 (2003), s. 100-108 ISSN 0932-0113 R&D Projects: GA AV ČR KJB6022305 Grant - others:GA FRVŠ(CZ) 1262/2002 Institutional research plan: CEZ:AV0Z6022909 Keywords : Nematoda * ultrastructure Subject RIV: EA - Cell Biology Impact factor: 1.000, year: 2003

  15. Comparative studies on intestine ultrastructure of third-stage larvae and adults of Cystidicoloides ephemeridarum (Nematoda, Cystidicolidae)

    Czech Academy of Sciences Publication Activity Database

    Frantová, Denisa; Moravec, František

    2004-01-01

    Roč. 94, č. 5 (2004), s. 377-383 ISSN 0932-0113 R&D Projects: GA AV ČR KJB6022305 Institutional research plan: CEZ:AV0Z6022909 Keywords : Nematoda * Cystidicolidae * ultrastructure Subject RIV: EG - Zoology Impact factor: 1.060, year: 2004

  16. Redescription of Parapharyngodon micipsae (Seurat 1917) (Nematoda Pharyngodonidae) from the new host Tarentola parvicarinata Joger 1980 (Squamata Gekkonidae)

    Czech Academy of Sciences Publication Activity Database

    Mašová, Š.; Baruš, Vlastimil; Hodová, I.; Koubek, Petr; Koubková, B.

    2009-01-01

    Roč. 22, č. 2 (2009), s. 243-255 ISSN 0394-6975 R&D Projects: GA AV ČR IAA6093404 Institutional research plan: CEZ:AV0Z60930519 Keywords : parasite * Nematoda * gecko * Senegal * West Africa Subject RIV: EG - Zoology Impact factor: 0.706, year: 2009

  17. A new species of Thelastomathidae (Nematoda) a parasite of Neocurtilla claraziana Saussure (Orthoptera, Gryllotalpidae) in Argentina.

    Science.gov (United States)

    Camino, Nora B; Maiztegui, Bárbara

    2002-07-01

    Gryllophila cephalobulata n. sp. (Nematoda, Thelastomatidae) a parasite of the mole cricket Neocurtilla claraziana (Orthoptera, Gryllotalpidae) isolated in Buenos Aires Province, is described and illustrated. It is characterized by cuticle annulated all along the length of the body; the first ring has 4 lobules, the second one has 14 lobules, the others rings are simple, the stoma is short and has 4 small teeth, the genital papillae are arranged in 5 pairs, of which 3 pairs are preanal and 2 pairs are postanal. The tail appendage of the male is long and filiform.

  18. Identifikasi Fusarium dan Nematoda Parasitik yang Berasosiasi dengan Penyakit Kuning Lada di Kalimantan Barat

    Directory of Open Access Journals (Sweden)

    Suryanti Suryanti

    2015-07-01

    Full Text Available Pepper (Piper nigrum, known as the “King of Spices” is one of the most important spices. In the international market, Indonesian pepper has high selling value, due to its flavor characteristics. Pepper yellowing disease is one of the most important disease that caused the decrease of pepper production and become the main problem in the cultivation of pepper in West Kalimantan. This research was conducted to determine the major causal agent of leaf yellowing disease of pepper. The Fusarium associated with diseased plant were isolated from the symptomatic plant and nematodes were isolated from the root with leaf yellowing symptom. The Fusarium isolates were cultured on agar medium, and the nematode was cultured on tomato plant. From diseased pepper in West Kalimantan, it was isolated 4 Fusarium isolates and plant parasitic nematode Meloidogyne. The result showed that H isolate of Fusarium was the most virulent isolate and identified asFusarium solani. The Meloidogyne was identified by the female perenial patern.The nematode was identified as Meloidogyne incognita. INTISARI Lada (Piper nigrum L. merupakan salah satu jenis rempah penting yang telah dikenal sebagai “King of Spices”. Di pasar internasional, lada Indonesia mempunyai daya jual tinggi karena cita rasanya yang khas. Salah satu kendala dalam budidaya lada adalah adanya penyakit kuning lada dan sampai saat ini menjadi masalah utama pada pertanaman lada di Kalimantan Barat. Informasi tentang patogen utama yang berinteraksi dengan penyakit kuning lada masih sangat terbatas, sehingga penelitian ini bertujuan untuk mengidentifikasi patogen utama yang berasosiasi dengan penyakit kuning lada. Isolasi Fusarium dilakukan dari batang lada dan isolasi nematoda dilakukan dari akar lada yang bergejala penyakit kuning di Kalimantan Barat. Fusarium hasil isolasi dikulturkan dalam medium agar, sedangkan nematoda hasil isolasi dikulturkan dalam akar tomat. Dari hasil isolasi berhasil didapatkan

  19. New species of Parapharyngodon (Nematoda: Pharyngodonidae) in Phymaturus spp. (Iguania: Liolaemidae) from Argentina.

    Science.gov (United States)

    Ramallo, Geraldine; Bursey, Charles; Castillo, Gabriel; Acosta, Juan Carlos

    2016-09-01

    Parapharyngodon sanjuanensis sp. nov. (Nematoda: Pharyngodonidae) from the large intestines of Phymaturus punae and Phymaturus williamsi (Squamata: Liolaemidae) from province of San Juan, Argentina, is described and illustrated. Parapharyngodon sanjuanensis sp. nov. is the 54th species assigned to the genus and the 8th from the Neotropical region. It differs from other species in the genus in that males possess 8 caudal papillae, 6 of which are large and pedunculate, 2 are small, almost inconspicuous; anterior lip echinate, posterior lip bilobate; females possess prominent vulva and short stiff tail spike.

  20. Proleptus acutus Dujardin, 1845 (Nematoda, Physalopteridae parasite of rays Zapteryx brevirostris Müller & Henle, 1841 (Rhinobatiformes, Rhinobatidae in Brazil Proleptus acutus Dujardin, 1845 (Nematoda, Physalopteridae parasita de raias Zapteryx brevirostris Müller & Henle, 1841 (Rhinobatiformes, Rhinobatidae no Brasil

    Directory of Open Access Journals (Sweden)

    R.J. Silva

    2008-12-01

    Full Text Available Relata-se a ocorrência de Proleptus acutus Dujardin, 1845 (Nematoda, Physalopteridae parasitando Zapteryx brevirostris Müller & Henle, 1841 (Rhinobatiformes, Rhinobatidae em Ubatuba, estado de São Paulo, Brasil. Vinte raias foram analisadas e P. acutus foi encontrado no estômago e intestinos de 14 (70% animais. Este é o primeiro relato da ocorrência dessa espécie de nematóide em Z. brevirostris no Brasil.

  1. Kemampuan Isolataktinomisetes Menghasilkan Enzim yang Dapatmerusak Kulit Telur Nematoda Puru-Akar Meloidogyne spp.

    Directory of Open Access Journals (Sweden)

    Bambang Rahayu TP

    2009-07-01

    Full Text Available Soil microbes including actinomycetes are known to produce various hydrolytic enzymes and antibiotics that can be used as biological controlling agents nematode. Therefore, surveys conducted in several areas in Yogyakarta, Central Java and East Java, to search for actinomycetes with chitinolytic, proteolytic, and chitino-proteolytic activity. Isolation of Actinomycetes produced 84 isolates, and most was obtained from shrimp head waste (26 isolates. After the selection based on their ability to hydrolyze chitines and protein in the medium, those whith the highest chitin and protein hydrolysis activity, are consecutive PSJ 27, TL 8, and TL 10 isolates. Test results of crude enzyme produced by selected isolates against root-knot nematode eggshell, showed that the isolates that have chitino-proteolytic activity (TL 10, is a highly effective isolate in damage eggshell. There are three types of damage to the nematode eggs. In the young eggs, crude enzyme preparation causing damage on vitelline and chitin layers. In the older eggs, preparation of crude enzyme cause premature hatching.   Sebagian mikrobia tanah, termasuk aktinomisetes, diketahui mampu menghasilkan berbagai enzim hidrolitik dan antibiotik yang dapat dimanfaatkan sebagai agens pengendalian hayati nematoda. Oleh karena itu,survei dilakukan di beberapa daerah di Yogyakarta, Jawa Tengah, dan Jawa Timur untuk mencari aktinomisetes yang mempunyai aktivitas kitinolitik, proteolitik dan kitino-proteolitik. Isolasi aktinomisetes menghasilkan 84 isolat, dan yang terbanyak diperoleh dari limbah kepala udang (26 isolat. Setelah dilakukan seleksi berdasarkan kemampuannya menghidrolisis kitin dan protein dalam medium, yang mempunyai aktivitas hidrolisis protein, kitin, protein dan kitin tertinggi berturut-turut adalah isolat PSJ 27, TL 8, dan TL 10. Hasil uji enzim kasar yang dihasilkan isolat terpilih terhadap perusakan kulit telur nematoda puru-akar menunjukkan bahwa isolat yang memiliki

  2. Tricholeiperia peruensis n. sp. (Nematoda, Molineidae) del quiróptero Lophostoma silvicolum occidentalis (Phyllostomidae) en Tumbes, Perú

    OpenAIRE

    Vargas, Marina; Martínez, Rosa; Tantaleán V, Manuel; Cadenillas, Richard; Pacheco, Víctor

    2008-01-01

    In the present work, Tricholeiperia peruensis. n sp. (Nematoda, Molineidae) is described on the basis of 25 collected specimens from 5 bat intestines of the Lophostoma silvicolum occidentalis species from Angostura, district of Pampas de Hospital, department of Tumbes, Peru. The new species is characterized by the morphology of the cephalic vesicle in the form of hood with recesses, for the size and form of spikes whose distal end of the external branch is divided in 2 from which the internal...

  3. Redescription of Dracunculus globocephalus Mackin, 1927 (Nematoda: Dracunculidae), a parasite of the snapping turtle, Chelydra serpentina.

    Science.gov (United States)

    Moravec, Frantisek; Little, M D

    2004-12-01

    Dracunculus globocephalus Mackin, 1927 (Nematoda: Dracunculoidea) is redescribed from specimens collected from the mesentery of the snapping turtle, Chelydra serpentina (L.), in Louisiana, USA. The use of scanning electron microscopy, applied for the first time in this species, made it possible to study details in the structure of the cephalic end and the arrangement of male caudal papillae that are difficult to observe under the light microscope. This species markedly differs from all other species of Dracunculus in having the spicules greatly unequal in size and shape, in the absence of a gubernaculum, and in the disposition of male caudal papillae. The validity of D. globocephalus is confirmed, but the above mentioned morphological differences are not sufficient for listing it in a separate genus. This is the first record of D. globocephalus in Louisiana.

  4. New species of Bakeria (Nematoda; Strongylida; Molineidae), new species of Falcaustra (Nematoda; Ascaridida; Kathlaniidae) and other helminths in Cnemaspis mcguirei (Sauria; Gekkonidae) from Peninsular Malaysia.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R; Grismer, L Lee

    2014-10-01

    Two new nematode species, Bakeria schadi sp. nov. and Falcaustra malaysiaia sp. nov. from the gastrointestinal tract of McGuire's rock gecko, Cnemaspis mcguirei (Sauria: Gekkonidae) collected in Peninsular Malaysia are described. The two species now assigned to Bakeria are separated on the bases of male bursa type and location of the excretory pore: type II in B. schadi sp. nov. and type I in B. bakeri; location of excretory pore, anterior to nerve ring in B. schadi sp. nov. and posterior to nerve ring in B. bakeri. Falcaustra malaysiaia sp. nov. is most similar to F. chabaudi, F. concinnae, F. condorcanquii, F. barbi, F. dubia, and F. tchadi in that these 7 species possess 1 pseudosucker, 1 median papilla plus 10 pairs caudal papillae, and spicules with lengths between 1 and 2 mm. F. barbi and F. tchadi lack adcloacal papillae; the remaining 5 species possess 1 pair of adcloacal papillae. Falcaustra chabaudi is known from Nearctic salamanders; F. concinnae from Nearctic turtles; F. condorcanquii from Neotropical frogs, F. dubia from Oriental frogs, and F. malaysiaia sp. nov. from Oriental geckos. Two additional species of Nematoda were found, Cosmocerca ornata and Meteterakis singaporensis. Cnemaspis mcguirei represents a new host record for Cosmocerca ornata and Meteterakis singaporensis.

  5. The nematode Diplotridena henryi (Nematoda : Diplotriaenoidea) as the possible cause of subcutaneous emphysema and respiratory insufficiency in a great tit (Parus major)

    Czech Academy of Sciences Publication Activity Database

    Literák, I.; Baruš, Vlastimil; Hauptmanová, K.; Halouzka, R.

    2003-01-01

    Roč. 40, č. 1 (2003), s. 23-25 ISSN 0440-6605 Institutional research plan: CEZ:AV0Z6093917 Keywords : Parus major * Nematoda * respiratory insufficiency Subject RIV: EG - Zoology Impact factor: 0.474, year: 2003

  6. Two new nematode species, Orientatractis campechensis n. sp. and Orientatractis chiapasensis n. sp. (Nematoda: Atractidae) from cichlid fishes in southern Mexico and Nicaragua

    Czech Academy of Sciences Publication Activity Database

    González-Solís, D.; Moravec, František

    2004-01-01

    Roč. 90, č. 6 (2004), s. 1443-1449 ISSN 0022-3395 R&D Projects: GA AV ČR IAA6022201 Institutional research plan: CEZ:AV0Z6022909 Keywords : Nematoda * Atractidae * parasites of fish Subject RIV: EA - Cell Biology Impact factor: 1.439, year: 2004

  7. Evolutionary relationships of Spirurina (Nematoda: Chromadorea: Rhabditida) with special emphasis on dracunculoid nematodes inferred from SSU rRNA gene sequences

    Czech Academy of Sciences Publication Activity Database

    Wijová, Martina; Moravec, František; Horák, Aleš; Lukeš, Julius

    2006-01-01

    Roč. 36, č. 9 (2006), s. 1067-1075 ISSN 0020-7519 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Nematoda * Spirurina * SSU rRNA gene sequences Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 3.337, year: 2006

  8. Soil nematodes (Nematoda) in the Voděradské bučiny National Nature Reserve (Czech Republic) - an overall characterization of the fauna

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2015-01-01

    Roč. 79, č. 3 (2015), s. 215-234 ISSN 1211-376X R&D Projects: GA ČR(CZ) GA206/93/0276 Institutional support: RVO:60077344 Keywords : soil zoology * ecology * Nematoda * diversity Subject RIV: EH - Ecology, Behaviour

  9. An annotated list of parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda and Nematoda) collected in groupers (Serranidae, Epinephelinae) in New Caledonia emphasizes parasite biodiversity in coral reef fish

    Czech Academy of Sciences Publication Activity Database

    Justine, J.-L.; Beveridge, I.; Boxshall, G.A.; Bray, R. A.; Moravec, František; Trilles, J.-P.; Whittington, I.D.

    2010-01-01

    Roč. 57, č. 4 (2010), s. 237-262 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Isopoda * Copepoda * Monogenea * Digenea * Cestoda * Nematoda * Serranidae * Epinephelinae * parasite biodiversity * coral reef * New Caledonia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.533, year: 2010

  10. Tricholeiperia peruensis n. sp. (Nematoda, Molineidae) del quiróptero Lophostoma silvicolum occidentalis (Phyllostomidae) en Tumbes, Perú

    OpenAIRE

    Vargas, Marina; Martínez, Rosa; Tantaleán, Manuel; Cadenillas, Richard; Pacheco, Víctor

    2008-01-01

    En el presente trabajo, se describe a Tricholeiperia peruensis n. sp. (Nematoda, Molineidae) en base a 25 especímenes colectados del intestino de 5 murciélagos de la especie Lophostoma silvicolum occidentalis de la localidad de Angostura, distrito Pampas de Hospital, departamento de Tumbes, Perú. La nueva especie se caracteriza por la morfología de la vesícula cefálica en forma de capuchón con escotaduras, por el tamaño y forma de las espículas cuyo extremo distal de la rama externa se divide...

  11. A new species of Biacantha (Nematoda: Molineidae), a parasite of the common vampire bat from the Yungas, Argentina.

    Science.gov (United States)

    Oviedo, Mirna C; Ramallo, Geraldine; Claps, Lucía E; Miotti, M Daniela

    2012-12-01

    A new species of Biacantha Wolfgang, 1954 (Nematoda: Molineidae), is described from the common vampire bat, Desmodus rotundus Geoffroy and St. Hilaire, 1810, from northwest Argentina. Biacantha normaliae n. sp. Oviedo, Ramallo, and Claps, is characterized by the disposition and number of ridges of the synlophe, the excretory pore located on a knob, 2 lateral processes on the tail of females, the male caudal bursa morphology, and lack of gubernaculum. This is the first species of nematode described in a vampire bat from Argentina.

  12. Morphology of chimpanzee pinworms, Enterobius (Enterobius) anthropopitheci (Gedoelst, 1916) (Nematoda: Oxyuridae), collected from chimpanzees, Pan troglodytes, on Rubondo Island, Tanzania.

    Science.gov (United States)

    Hasegawa, Hideo; Ikeda, Yatsukaho; Fujisaki, Akiko; Moscovice, Liza R; Petrzelkova, Klara J; Kaur, Taranjit; Huffman, Michael A

    2005-12-01

    The chimpanzee pinworm, Enterobius (Enterobius) anthropopitheci (Gedoelst, 1916) (Nematoda: Oxyuridae), is redescribed based on light and scanning electron microscopy of both sexes collected from the feces of chimpanzees, Pan troglodytes, of an introduced population on Rubondo Island, Tanzania. Enterobius (E.) anthropopitheci is characterized by having a small body (males 1.13-1.83 mm long, females 3.33-4.73 mm long), a rather straight spicule with a ventral membranous formation in males, double-crested lateral alae in females, small eggs (53-58 by 24-28 microm), and a smooth eggshell with 3 longitudinal thickenings. Morphological comparison is made between the present and previous descriptions.

  13. First Blindness Cases of Horses Infected with Setaria Digitata (Nematoda: Filarioidea) in the Republic of Korea.

    Science.gov (United States)

    Shin, Jihun; Ahn, Kyu-Sung; Suh, Guk-Hyun; Kim, Ha-Jung; Jeong, Hak-Sub; Kim, Byung-Su; Choi, Eunsang; Shin, Sung-Shik

    2017-12-01

    Ocular setariases of cattle were reported but those of equine hosts have never been reported in the Republic of Korea (Korea). We found motile worms in the aqueous humor of 15 horses (Equus spp.) from 12 localities in southern parts of Korea between January 2004 and November 2017. After the affected animals were properly restrained under sedation and local anesthesia, 10 ml disposable syringe with a 16-gauge needle was inserted into the anterior chamber of the affected eye to successfully remove the parasites. The male worm that was found in 7 of the cases showed a pair of lateral appendages near the posterior terminal end of the body. The papillar arrangement was 3 pairs of precloacal, a pair of adcloacal, and 3 pairs of postcloacal papillae, plus a central papilla just in front of the cloaca. The female worms found in the eyes of 8 horses were characterized by the tapering posterior terminal end of the body with a smooth knob. Worms were all identified as Setaria digitata (von Linstow, 1906) by the morphologic characteristics using light and electron microscopic observations. This is the first blindness cases of 15 horses infected with S. digitata (Nematoda: Filarioidea) in Korea.

  14. Uncinaria sanguinis sp. n. (Nematoda: Ancylostomatidae) from the endangered Australian sea lion, Neophoca cinerea (Carnivora: Otariidae).

    Science.gov (United States)

    Marcus, Alan D; Higgins, Damien P; Slapeta, Jan; Gray, Rachael

    2014-06-01

    This study investigates the identity of hookworms parasitising the Australian sea lion, Neophoca cinerea (Péron), from three colonies in South Australia, Australia. The Australian sea lion is at risk of extinction because its population is small and genetically fragmented. Using morphological and molecular techniques, we describe a single novel species, Uncinaria sanguinis sp. n. (Nematoda: Ancylostomatidae). The new species is most similar to hookworms also parasitic in otariid hosts, Uncinaria lucasi Stiles, 1901 and Uncinaria hamiltoni Baylis, 1933. Comparative morphometrics offered limited utility for distinguishing between species within this genus whilst morphological features and differences in nuclear ribosomal DNA sequences delineated U. sanguinis sp. n. from named congeners. Male specimens of U. sanguinis sp. n. differ from U. lucasi and U. hamiltoni by relatively shorter anterolateral and externodorsal rays, respectively, and from other congeners by the relative lengths and angulations of bursal rays, and in the shape of the spicules. Female specimens of U. sanguinis sp. n. are differentiated from Uncinaria spp. parasitic in terrestrial mammals by differences in vulval anatomy and the larger size of their eggs, although are morphologically indistinguishable from U. lucasi and U. hamiltoni. Molecular techniques clearly delimited U. sanguinis sp. n. as a distinct novel species. Obtaining baseline data on the parasites of wildlife hosts is important for the investigation of disease and the effective implementation and monitoring of conservation management.

  15. Anisakis simplex (Nematoda: Anisakidae from horse mackerel (Trachurus trachurus in Atlantic coast of Morocco

    Directory of Open Access Journals (Sweden)

    Nizar Shawket

    2017-08-01

    Full Text Available Objective: To focus on the description of the Anisakis simplex (A. simplex parasites of Trachurus trachurus (Linnaeus, 1758 from the Atlantic coast of Mehdia (Kenitra, Morocco from December 2014 to November 2015. Methods: A total of 1 012 Trachurus trachurus (Linnaeus, 1758 obtained from commercial fishing were performed autopsy for their parasitic Nematoda. Then 6 695 specimens of A. simplex were collected from their abdominal cavity. These parasites were attached on different organs particularly on ovaries and testes. All parasites were counted, measured and photographed under microscopy. Results: The infection levels of fishes by larval A. simplex are expressed by prevalence (35.28%, mean intensity (18.75 and abundance (6.6. The effect of parasitism did not show a significant negative impact on the condition of the examined fishes. Conclusions: Significant positive correlations were found between host length and A. simplex occurrence, and abundance. The variation observed in the infection levels was discussed within the seasons and climatic change.

  16. Evidence of morphine like substance and μ-opioid receptor expression in Toxacara canis (Nematoda: Ascaridae).

    Science.gov (United States)

    Golabi, Mostafa; Naem, Soraya; Imani, Mehdi; Dalirezh, Nowruz

    2016-01-01

    Toxocara canis (Nematoda: Ascaridae) is an intestinal nematode parasite of dogs, which can also cause disease in humans. Transmission to humans usually occurs because of direct contact with T. canis eggs present in soil contaminated with the feces of infected dogs. This nematode has extraordinary abilities to survive for many years in different tissues of vertebrates, and develop to maturity in the intestinal tract of its definitive host. Survival of parasitic nematodes within a host requires immune evasion using complicated pathways. Morphine-like substance, as well as opioids, which are known as down regulating agents, can modulate both innate and acquired immune responses, and let the parasite survives in their hosts. In the present study, we aimed to find evidences of morphine-like substance and µ-opiate receptor expression in T. canis , using high performance liquid chromatography (HPLC) and reverse transcription polymerase chain reaction (RT-PCR). The results indicated that T. canis produced morphine-like substances at the level of 2.31± 0.26 ng g -1 wet weight, and expressed µ-opiate receptor as in expected size of 441 bp. According to our findings, it was concluded that T. canis , benefits using morphine-like substance to modulate host immunity.

  17. Nematoda: Dorylaimida

    African Journals Online (AJOL)

    1992-04-02

    sedimenta- tion method (Loubser 1985), killed by gentle heat, fixed in. FAA, processed into glycerine by Thome's slow method and mounted on permanent slides. Measurements and draw- ings were made with the aid of a Zeiss ...

  18. Nematoda: Dorylaimidae

    African Journals Online (AJOL)

    Box 524, Auckland Park, 2006. A.J. Meyer. Department of Entomology and Nematology, University of Stellenbosch, Stellenbosch, 7600. Received 5 December 1994; accepted 15 May 1995. Calcaridorylaimus sirgeli n.sp. is described from Cape Fynbos in mountains in the south~western Cape Prov- ince. It differs from all ...

  19. Nematoda: Tylenchidae

    African Journals Online (AJOL)

    1996-02-05

    Feb 5, 1996 ... 149-175 body annules intersected by 14 longitudinal lines (incisures), protruding lateral fields marked by four incisures, and an ... line, while the two outer lines are mere continuations of lon- gitudinal lines from further ..... Andrassy (1982), is possibly a printer's error, since no other. Neotliada (1o(1ulation ...

  20. Descriptions of Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina) from Haryana, India.

    Science.gov (United States)

    Tomar, V V S; Somvanshi, Vishal S; Bajaj, Harish K

    2015-03-01

    Two different nematodes were isolated from the bark of Albizia lebbeck trees; one from insect infested and another from noninfested, healthy tree. Based on the biological, morphological, and molecular evidences, the nematodes are described as Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina). Deladenus albizicus n. sp., isolated from insect-infested tree, multiplied on the fungus Nigrospora oryzae. Myceliophagous females of this nematode reproduced by parthenogenesis and spermathecae were indistinct. Infective females, readily produced in the cultures, are dorsally curved. Only one type of males containing small-sized sperms in their genital tracts were produced in the culture. Myceliophagous females: L = 0.75 to 1.71 mm, a = 32.3 to 50.8, b = 9.3 to 11.2, b' = 5.2 to 7.3, c = 27.2 to 35.6, V = 91.0 to 93.3, c' = 2.0 to 2.9, stylet = 11 to 12 µm, excretory pore in the region of median pharyngeal bulb, 43 to 47 µm anterior to hemizonid. Deladenus processus n. sp., isolated from bark of healthy A. lebbeck tree, was cultured on Alternaria alternata. Myceliophagous females reproduced by amphimixis and their spermathecae contained rounded sperms. Infective females were never produced, even in old cultures. Myceliophagous females: L = 0.76 to 0.99 mm, a = 34 to 49, b = 13.3 to 17.7, b' = 3.8 to 5.8, c = 19.6 to 22.8, V = 92.2 to 93.5, c' = 2.7 to 3.5, stylet = 6 to 7 µm, excretory pore in the proximity of hemizonid, tail conoid, tapering from both sides to a long pointed central process. It is proposed to classify Deladenus species in three groups: durus, siricidicola, and laricis groups based on female and spermatogonia dimorphism, mode of reproduction, and insect parasitism.

  1. A preliminary survey of soil nematodes (Nematoda) in inverse gorges in the České Švýcarsko National Park (Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2010-01-01

    Roč. 74, - (2010), s. 39-48 ISSN 1211-376X. [Central European Workshop on Soil Zoology /10./. České Budějovice, 21.04.2009-24.04.2009] Grant - others:EEA Financial Mechanism(BE) CZ0048 Institutional research plan: CEZ:AV0Z60660521 Keywords : soil zoology * ecology * Nematoda Subject RIV: EH - Ecology, Behaviour

  2. Phylogenetic relationships of some spirurine nematodes (Nematoda: Chromadorea: Rhabditida: Spirurina) parasitic in fishes inferred from SSU rRNA gene sequences

    Czech Academy of Sciences Publication Activity Database

    Černotíková, Eva; Horák, Aleš; Moravec, František

    2011-01-01

    Roč. 58, č. 2 (2011), s. 135-148 ISSN 0015-5683 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Nematoda * Spirurina * SSU rRNA * phylogeny * taxonomy Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.812, year: 2011 http://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=21981

  3. Draft Whole-Genome Sequence of Serratia sp. Strain TEL, Associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae) Isolated from a Grassland in South Africa.

    Science.gov (United States)

    Lephoto, Tiisetso E; Featherston, Jonathan; Gray, Vincent M

    2015-07-09

    Here, we report on the draft genome sequence of Serratia sp. strain TEL, associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae, KM492926) isolated from a grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Serratia sp. strain TEL has a genome size of 5,000,541 bp with 4,647 genes and a G+C content of 59.1%. Copyright © 2015 Lephoto et al.

  4. Draft Whole-Genome Sequence of Serratia sp. Strain TEL, Associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae) Isolated from a Grassland in South Africa

    OpenAIRE

    Lephoto, Tiisetso E.; Featherston, Jonathan; Gray, Vincent M.

    2015-01-01

    Here, we report on the draft genome sequence of Serratia sp. strain TEL, associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae, KM492926) isolated from a grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Serratia sp. strain TEL has a genome size of 5,000,541 bp with 4,647 genes and a G+C content of 59.1%.

  5. A new genus and species of philometrid (Nematoda) from the subcutaneous tissue of the crevalle jack, Caranx hippos (Osteichthyes), from the southern Gulf of Mexico

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Montoya-Mendoza, J.; Salgado-Maldonado, G.

    2008-01-01

    Roč. 96, č. 6 (2008), s. 1346-1350 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Nematoda * Philometridae * Caranx * Caranginema * Gulf of Mexico Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.165, year: 2008

  6. On a New Species of Hysterothylacium (Nematoda: Anisakidae from Cauque mauleanum (Pisces: Atherinidae by Brightfield and Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Torres Patricio

    1998-01-01

    Full Text Available Hysterothylacium geschei n. sp. (Nematoda, Anisakidae is described from the intestine of Cauque mauleanum (Steindachner (Pisces: Atherinidae from Lake Panguipulli (39º43'S; 72º13'W, Chile. Eleven (78.6% out of 14 fish were infected, with a mean intensity (range of 14.4 (1-55 worms. The new species can be differentiated from the two previously described species of freshwater fishes from South America by the presence of lateral alae, the number of caudal papillae, and the length of the spicules, oesophagus, intestinal caecum, distance vulva-anterior extremity and the length ratio intestinal caecum: ventricular appendix. From the fishes examined in Lake Panguipulli, including the introduced salmonid species Oncorhynchus mykiss (Walbaum and the authochthonous species Basilichthys australis Eigenmann (Atherinidae and Percichthys trucha (Valenciennes (Percichthyidae, only one specimen of P. trucha was found parasitized by a third-stage larva of this species.

  7. New Data on the Morphology and Distribution of Oswaldocruzia Skrjabini (Nematoda, Molineidae)

    OpenAIRE

    Svitin R. S.

    2015-01-01

    Шестнадцать экземпляров Oswaldocruzia (Nematoda, Molineidae) были обнаружены в материале от Zootoca vivipara Lichtenshtein, 1823 собранном на территории Украины. Нематоды были определены как O. skrjabini Travassos, 1917 на основании наличия развитых латеральных крыльев и вида хозяина. Представлено дополненное описание вида с новыми морфологическими признаками, морфометрией и распространением в Украине. Oswaldocruzia skrjabini отличается от других видов рода наличием дополнительных отростков н...

  8. New Data on the Morphology and Distribution of Oswaldocruzia Skrjabini (Nematoda, Molineidae

    Directory of Open Access Journals (Sweden)

    Svitin R. S.

    2015-10-01

    Full Text Available Шестнадцать экземпляров Oswaldocruzia (Nematoda, Molineidae были обнаружены в материале от Zootoca vivipara Lichtenshtein, 1823 собранном на территории Украины. Нематоды были определены как O. skrjabini Travassos, 1917 на основании наличия развитых латеральных крыльев и вида хозяина. Представлено дополненное описание вида с новыми морфологическими признаками, морфометрией и распространением в Украине. Oswaldocruzia skrjabini отличается от других видов рода наличием дополнительных отростков на ветках fork и shoe спикул.

  9. Linear distribution of nematodes in the gastrointestinal tract of tracer lambs.

    Science.gov (United States)

    Makovcová, Katerina; Langrová, Iva; Vadlejch, Jaroslav; Jankovská, Ivana; Lytvynets, Andriy; Borkovcová, Marie

    2008-12-01

    Forty-eight tracer lambs were killed in 2004-2007. The abomasum, duodenum, small intestine (jejunum and ileum), colon and caecum were collected and processed for parasites enumeration and identification-mucosal scrapings of both abomasums and intestines were digested. Out of 48 gastrointestinal tracts examined, all were found to be positive for nematode infection. Seventeen species of gastrointestinal nematodes were recovered: Bunostomum trigonocephalum, Cooperia curticei, Haemonchus contortus, Chabertia ovina, Nematodirus battus, Nematodirus filicollis, Oesophagostomum venulosum, Teladorsagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Strongyloides papillosus, Trichuris ovis, Trichuris globulosa, Trichuris skrjabini and Skrjabinema ovis. All species were searched for in the entire gastrointestinal tract. Six species of nematodes were recovered from abnormal sites, naturally in small numbers of lambs as well as in small amounts: Nematodirus battus in the abomasums (6.67% of lambs), N. filicollis in the caecum and in the colon (%4 and 8%, respectively), T. axei in the colon (9.52%), T. colubriformis in the colon (13.89%), T. vitrinus in the caecum (16.67%), in the colon (20.00%) and in the abomasum (3.33%). T. ovis was found in one case in the small intestine.

  10. Philometra thaiensis sp. nov. (Nematoda, Philometridae) from Tetraodon palembangensis and T. fluviatilis (Pisces) from fresh waters in Thailand, with a key to Philometra spp. parasitic in the host's abdominal cavity

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Fiala, Ivan; Dyková, Iva

    2004-01-01

    Roč. 49, č. 4 (2004), s. 319-324 ISSN 1230-2821 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z6022909 Keywords : Nematoda * Philometra * parasite Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.560, year: 2004

  11. Distribution of trophic groups of soil nematodes (Nematoda) and soil food web condition in inverse gorges in the České Švýcarsko National Park (Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2013-01-01

    Roč. 77, č. 2 (2013), s. 87-101 ISSN 1211-376X Grant - others:EEA Financial Mechanism(NO) CZ0048 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : soil zoology * ecology * Nematoda * trophic group * Bohemian Switzerland National Park Subject RIV: EH - Ecology, Behaviour

  12. Free-living nematodes (Nematoda) of the Rokytská Slať and the Chalupská Slať peat bogs in the Bohemian Forest (Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2015-01-01

    Roč. 79, č. 3 (2015), s. 199-213 ISSN 1211-376X R&D Projects: GA ČR GA206/99/1416 Institutional support: RVO:60077344 Keywords : soil zoology * ecology * Nematoda * diversity * peatland Subject RIV: EH - Ecology, Behaviour

  13. Current distribution of Achatina fulica, in the state of São Paulo including records of Aelurostrongylus abstrusus (Nematoda) larvae infestation.

    Science.gov (United States)

    Ohlweiler, Fernanda Pires; Guimarães, Marisa Cristina de Almeida; Takahashi, Fernanda Yoshika; Eduardo, Juliana Manas

    2010-01-01

    The currently known distribution range of Achatina fulica Bowdich, 1822, in the state of São Paulo, Brazil, is presented. The record of A. fulica naturally infested with Aelurostrongylus abstrusus larvae (Railliet, 1898) (Nematoda: Metastrongylidae) can be found in the city of Guaratinguetá. It was found A. fulica with Metastrongylidae larvae without known medical and veterinary importance in the cities of Carapicuíba, Embu-Guaçu, Itapevi, São Caetano do Sul, São Paulo and Taboão da Serra.

  14. Redescription of Enterobius (Enterobius) macaci Yen, 1973 (Nematoda: Oxyuridae: Enterobiinae) based on material collected from wild Japanese macaque, Macaca fuscata (Primates: Cercopithecidae).

    Science.gov (United States)

    Hasegawa, Hideo; Sato, Hiroshi; Torii, Harumi

    2012-02-01

    Enterobius (Enterobius) macaci Yen, 1973 (Nematoda: Oxyuridae: Enterobiinae) was collected from a Japanese macaque, Macaca fuscata, in Nara and Yamaguchi Prefectures, Honshu Island, Japan, for the first time. A redescription is presented along with DNA sequence data. This pinworm is a typical member of the subgenus Enterobius and is characteristic in the spicule morphology, being readily distinguished from other congeners. Phylogenetic analyses based on 18S ribosomal RNA gene (rDNA) and mitochondrial DNA (mtDNA) Cox1 gene assign its position in the pinworm lineage adapted to the Old World primates, showing divergence before the splitting of the chimpanzee and human pinworms.

  15. Tricholeiperia peruensis n. sp. (Nematoda, Molineidae del quiróptero Lophostoma silvicolum occidentalis (Phyllostomidae en Tumbes, Perú

    Directory of Open Access Journals (Sweden)

    Marina Vargas

    2008-12-01

    Full Text Available En el presente trabajo, se describe a Tricholeiperia peruensis n. sp. (Nematoda, Molineidae en base a 25 especímenes colectados del intestino de 5 murciélagos de la especie Lophostoma silvicolum occidentalis de la localidad de Angostura, distrito Pampas de Hospital, departamento de Tumbes, Perú. La nueva especie se caracteriza por la morfología de la vesícula cefálica en forma de capuchón con escotaduras, por el tamaño y forma de las espículas cuyo extremo distal de la rama externa se divide en dos, de las cuales la rama interna sufre una nueva división; por la disposición de las costillas de la bolsa copulatríz donde, entre otros detalles, las antero y postero-ventrales están separadas y las externo-dorsales nacen cerca de la parte media del tronco dorsal y son gruesas.

  16. Liver histopathology in the cane toad, Rhinella marina (Amphibia: Bufonidae), induced by Ortleppascaris sp. larvae (Nematoda: Ascarididae).

    Science.gov (United States)

    Silva, Jefferson P E; da Silva, Djane C B; Melo, Francisco T V; Giese, Elane G; Furtado, Adriano P; Santos, Jeannie N

    2013-04-01

    Exposure to parasites is considered to be an important factor in the development of many diseases and histopathologies which are the result of the parasite-host interaction. The present study evaluated the impact of natural infection by larvae of Ortleppascaris sp. (Nematoda: Ascaridida) in the liver of the cane toad Rhinella marina (Linnaeus, 1758). Larvae were encysted in nodules delimited by collagenous fibers and fibroblasts or freely within the hepatic parenchyma, provoking a clear response from the host. The histological examination of the liver revealed viable larvae in a number of different developmental stages, as well as cysts filled with amorphous material and cell residues and surrounded by dense fibrotic tissue. The infection of the liver by these larvae induces a significant increase in the area occupied by melanomacrophages and a reduction or deficit in the vascularization of the liver, hypertrophy of the hepatocytes, vacuolar bodies, and cytoplasmatic granules. Focal concentrations of inflammatory infiltrates were observed enclosing the unencapsulated early-stage larvae. These results indicate that infection by Ortleppascaris sp. induces severe physiological problems and histopathological lesions in the liver of R. marina .

  17. Characterization of the complete mitochondrial genome of Ortleppascaris sinensis (Nematoda: Heterocheilidae) and comparative mitogenomic analysis of eighteen Ascaridida nematodes.

    Science.gov (United States)

    Zhao, J H; Tu, G J; Wu, X B; Li, C P

    2018-05-01

    Ortleppascaris sinensis (Nematoda: Ascaridida) is a dominant intestinal nematode of the captive Chinese alligator. However, the epidemiology, molecular ecology and population genetics of this parasite remain largely unexplored. In this study, the complete mitochondrial (mt) genome sequence of O. sinensis was first determined using a polymerase chain reaction (PCR)-based primer-walking strategy, and this is also the first sequencing of the complete mitochondrial genome of a member of the genus Ortleppascaris. The circular mitochondrial genome (13,828 bp) of O. sinensis contained 12 protein-coding, 22 transfer RNA and 2 ribosomal RNA genes, but lacked the ATP synthetase subunit 8 gene. Finally, phylogenetic analysis of mtDNAs indicated that the genus Ortleppascaris should be attributed to the family Heterocheilidae. It is necessary to sequence more mtNDAs of Ortleppascaris nematodes in the future to test and confirm our conclusion. The complete mitochondrial genome sequence of O. sinensis reported here should contribute to molecular diagnosis, epidemiological investigations and ecological studies of O. sinensis and other related Ascaridida nematodes.

  18. Two new species of philometrid nematodes (Nematoda: Philometridae) in Epinephelus coioides (Hamilton, 1822) from the South Bali Sea, Indonesia.

    Science.gov (United States)

    Dewi, Kartika; Palm, Harry W

    2013-01-25

    Based on light and scanning electron microscopy, two new species of philometrid nematodes, Spirophilometra endangae sp. nov. and Philometra epinepheli sp. nov. (Nematoda: Dracunculoidea: Philometridae) are described from Epinephelus coioides (Hamilton, 1822) (Perciformes: Serranidae) from the South Bali Sea, Indonesia. Spirophilometra endangae sp. nov. was isolated from the fins of E. coioides. The new species can be distinguished from the most closely related S. eichleri Parukhin, 1971 by a larger total body length and the site of infection in the host. The new species differs from S. centropomi (Caballero, 1974) also in the larger body size of the gravid females and the site of infection in the host. S. en-dangae sp. nov. differs from S. pacifica (Moravec, Santana-Pineros, Gonzales-Solis & Torres-Huerta, 2007) in the struc-ture and arrangement of the spines on the middle part of the body, the infection site of the worm, the type host and the zoogeographical host distribution. Philometra epinepheli sp. nov. differs from all other Philometra spp. congeners so far recorded from Ephinepelus groupers in the total body length and the site of infection. This is the first opercula-infecting species of Philometra described from the fish family Serranidae.

  19. Trichostrongylus colubriformis rDNA polymorphism associated with arrested development

    Czech Academy of Sciences Publication Activity Database

    Langrová, I.; Zouhar, M.; Vadlejch, J.; Borovský, M.; Jankovská, I.; Lytvynets, Andrej

    2008-01-01

    Roč. 103, č. 2 (2008), s. 401-403 ISSN 0932-0113 Institutional research plan: CEZ:AV0Z50110509 Keywords : arrested development * polymorphism * rDNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.473, year: 2008

  20. Effect of mini-sprinkler irrigation system on Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae infective juvenile Efeito do sistema de irrigação por microaspersão em juvenis infectantes de Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae

    Directory of Open Access Journals (Sweden)

    Juan Carlos Lara

    2008-01-01

    Full Text Available Entomopathogenic nematodes (EPNs are currently being used as successful biological control agents of soil-dwelling insect pests. Previous field and greenhouse studies demonstrated that application techniques and non-biotic factors (temperature and pressure have a significant effect on EPNs efficacy. The objective of this study was to evaluate the influence of an irrigation spray application system on the viability, infectivity and host search capability of Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae infective juveniles (IJ. Two assays were proposed. Their viability was evaluated under the microscope after the IJ passed through the irrigation system. Infectivity on Galleria mellonella larvae, and host search capability, as evidenced by larval mortality, were evaluated in containers (Experiment 1. In the field (Experiment 2, mortality of G. mellonella larvae was evaluated under different nematode concentrations (0, 100,000, 300,000 and 500,000 IJ per tree. No differences were recorded on the viability, infectivity and host search capability of the IJ in Experiment 1. In Experiment 2, differences were recorded among the different concentrations used (p Nematóides entomopatogênicos (NEPs vêm sendo usados com sucesso como agentes do controle biológico de pragas de solo. Estudos anteriores mostraram que técnicas de aplicação e fatores abióticos (temperatura e pressão afetam a eficiência dos NEPs em testes de campo e casa-de-vegetação. O objetivo deste trabalho foi avaliar a influência de condições geradas por um sistema de irrigação por microaspersão, na viabilidade, infectividade e na capacidade de busca de hospedeiros nos juvenis infectantes (JI de Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae. Dois experimentos foram propostos. A viabilidade dos juvenis infectantes (JI foi avaliada no microscópio imediatamente após sua passagem pelo sistema de irrigação. A infectividade e a capacidade de busca pelo

  1. Distribution of Thelastomatoid Nematodes (Nematoda: Oxyurida) in Endemic and Introduced Cockroaches on the Galápagos Island Archipelago, Ecuador.

    Science.gov (United States)

    Sinnott, Devinn; Carreno, Ramon A; Herrera, Henri

    2015-08-01

    The thelastomatoid pinworm fauna (Nematoda: Oxyurida: Thelastomatoidea) was surveyed in 3 endemic species and 6 introduced species of cockroach hosts (Insecta: Blattaria) in the Galápagos Islands, Ecuador. A total of 658 host specimens were examined from preserved collections that had been collected between 1966 and 2003 from 7 islands in the archipelago. Eight species of pinworms were identified from these cockroach hosts, including the dominant species Cephalobellus ovumglutinosus and a Severianoia sp. as well as Leidynema appendiculata, Hammerschmidtiella diesingi, an unidentified Cephalobellus species resembling Cephalobellus magalhaesi, an unidentified Protrellus species closely resembling Protrellus shamimi, and an undescribed Blattophila sp. Five new host records are identified for C. ovumglutinosus including the endemic Galápagos cockroaches Chorisoneura carpenteri, Ischnoptera snodgrassii, and Ischnoptera santacruzensis. These endemics were also infected with an undescribed Blatticola sp. Other species recorded resemble known pinworms from other hosts around the world. Prevalence between islands and between host species was variable, but total prevalence for individual pinworm species was consistently low (<10%). A single host specimen examined was infected with more than 1 pinworm species; otherwise only a single species was observed in each infected host. At least 1 introduced pinworm species carried to the islands via invasive cockroach hosts was present in endemic host species, but several globally widespread introduced pinworm species were absent from endemic cockroaches. Santa Cruz was inhabited by the greatest number of pinworm species, likely due to a higher rate of invasive host introduction. This survey, the first from this region, showed that the distribution and transmission of pinworms in the Galápagos Islands is complex and may provide future models of invertebrate dispersal and speciation in an ecosystem already rich with examples of

  2. Redescription, systematic status and molecular characterisation of Multicaecum heterotis Petter, Vassiliadès et Marchand, 1979 (Nematoda: Heterocheilidae), an intestinal parasite of Heterotis niloticus (Osteichthyes: Arapaimidae) in Africa

    Czech Academy of Sciences Publication Activity Database

    Mašová, Š.; Moravec, František; Baruš, V.; Seifertová, M.

    2010-01-01

    Roč. 57, č. 4 (2010), s. 280-288 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Grant - others:GA ČR(CZ) GD526/09/H025 Institutional research plan: CEZ:AV0Z60220518 Keywords : Nematoda * Brevimulticaecum * Multicaecum * Senegal * Sudan * Africa * Heterotis * barcoding * 18S rDNA sequences * ITS2 sequences Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.533, year: 2010

  3. Current distribution of Achatina fulica, in the State of São Paulo including records of Aelurostrongylus abstrusus (Nematoda larvae infestation Distribuição atual de Achatina fulica Bowdich, 1822 no Estado de São Paulo com registro de infestação por larvas de Aelurostrongylus abstrusus (Nematoda

    Directory of Open Access Journals (Sweden)

    Fernanda Pires Ohlweiler

    2010-08-01

    Full Text Available The currently known distribution range of Achatina fulica Bowdich, 1822, in the state of São Paulo, Brazil, is presented. The record of A. fulica naturally infested with Aelurostrongylus abstrusus larvae (Railliet, 1898 (Nematoda: Metastrongylidae can be found in the city of Guaratinguetá. It was found A. fulica with Metastrongylidae larvae without known medical and veterinary importance in the cities of Carapicuíba, Embu-Guaçu, Itapevi, São Caetano do Sul, São Paulo and Taboão da Serra.É apresentada a distribuição de Achatina fulica Bowdich, 1822 no Estado de São Paulo, Brasil. É fornecido o registro de A. fulica naturalmente infestada por larvas de Aelurostrongylus abstrusus (Railliet, 1898 (Nematoda: Metastrongylidae no município de Guaratinguetá. Foi encontrada A. fulica portando larvas de Metastrongylidae sem importância médica e veterinária conhecida nos municípios de Carapicuíba, Embu Guaçu, Itapevi, São Caetano do Sul, São Paulo e Taboão da Serra.

  4. Ichthyouris voltagrandensis n.sp. (Nematoda: Pharyngodonidae) from Myleus tiete Eigenmann & Norris, 1900 (Osteichthyes: Characidae) in the Volta Grande Reservoir, MG, Brazil.

    Science.gov (United States)

    Martins, M L; Yoshitoshi, E R; Umekita, H

    2001-05-01

    The present work studied helminth parasites of "pacu-manteiga", Myleus tiete (Osteichthyes: Characidae) from Volta Grande Reservoir, MG, Brazil. Fishes with 142.6 +/- 24.7 g weight and 17.3 +/- 1.0 cm total length were collected. Five out six analysed fish (prevalence 83.3%) were parasitized in the intestine with an average of 535.6 +/- 334.6 oxyurid nematodes per fish. The helminth was identified as Ichthyouris voltagrandensis n.sp. (Nematoda: Pharyngodonidae). It differs from I. brasiliensis (Moravec et al., 1992a) by the absence of lateral alae, higher measures of tail and caudal alae of males, esophageal isthmus length, distance of excretory pore from anterior end and spicule length. In addition, eggs were provided by two long filaments in just one pole and by the first time the authors observed flagellate spermatozoa from dissected males. The authors exposes comparative table of measures of the five described species of the genus Ichthyouris.

  5. A multilocus approach to assessing co-evolutionary relationships between Steinernema spp. (Nematoda: Steinernematidae) and their bacterial symbionts Xenorhabdus spp. (gamma-Proteobacteria: Enterobacteriaceae).

    Science.gov (United States)

    Lee, Ming-Min; Stock, S Patricia

    2010-09-01

    Nematodes of the genus Steinernema Travassos, 1927 (Nematoda: Steinernematidae) and their associated bacteria, Xenorhabdus spp. (gamma-Proteobacteria), are an emergent model of terrestrial animal-microbe symbiosis. Interest in this association initially arose out of their potential as biocontrol agents against insect pests, but, despite advances in their field application and the growing popularity of this model system, relatively little has been published to uncover the evolutionary facets of this beneficial partnership. This study adds to the body of knowledge regarding nematode-bacteria symbiosis by proposing a possible scenario for their historical association in the form of a cophylogenetic hypothesis. Topological and likelihood based testing methods were employed to reconstruct a history of association between 30 host-symbiont pairs and to gauge the level of similarity between their inferred phylogenetic patterns.

  6. Digenea, Nematoda, Cestoda, and Acanthocephala, parasites in Potamotrygonidae (Chondrichthyes from the upper Paraná River floodplain, states of Paraná and Mato Grosso do Sul, Brazil.

    Directory of Open Access Journals (Sweden)

    Pavanelli, G. C.

    2008-01-01

    Full Text Available The present paper represents the first study on the endoparasitic fauna of Potamotrygon falkneri and P.motoro in the upper Paraná River floodplain. Fishes were collected by fishing rod and gillnetting in different stations ofthe floodplain, from March, 2005 to September, 2006. Parasites were sampled, fixed and preserved according tospecialized literature. About half of the analyzed fish were parasitized by at least one of the following species ofendoparasites: Clinostomum complanatum, Genarchella sp. and Tylodelphys sp. (metacercaria (Digenea;Acanthobothrium regoi, Rhinebothrium paratrygoni, Paroncomegas araya and Potamotrygonocestus travassosi(Cestoidea; Brevimulticaecum sp. (larva, Cucullanus sp., Echinocephalus sp. and Spinitectus sp. (Nematoda; andQuadrigyrus machadoi (Acanthocephala. Some species were already registered in Chondrichthyes and others werepreviously recorded in Osteichthyes from the study area. The study listed ten new records of parasites in the host P.falkneri, one new record in the host P. motoro and five new records in the locality upper Paraná River.

  7. First record of larvae of Hysterothylacium (Nematoda: Anisakidae with zoonotic potential in the pirarucu Arapaima gigas (Osteichthyes: Arapaimidae from South America

    Directory of Open Access Journals (Sweden)

    S. M. Andrade-Porto

    Full Text Available Abstract Third-stage larvae (L3 of Hysterothylacium sp. were collected by the first time in juveniles of pirarucu Arapaima gigas farmed in the Rio Preto da Eva, Amazonas state. Ninety-eight (98 out of 100 examined fish showed to be parasitized. Five hundred and ninety larvae of Hysterothylacium sp. were collected from the intestines, stomach and pyloric caeca. The mean intensity of parasite indexes was 6.02 (±5.75 ranging from 1 to 40 larvae per host and the mean abundance was 5.9 (±5.76. The A. gigas is the new host record for larvae of Hysterothylacium sp. in Brazil, and this is the first record of larvae of Hysterothylacium (Nematoda: Anisakidae with zoonotic potential in the pirarucu from South America.

  8. First record of larvae of Hysterothylacium (Nematoda: Anisakidae) with zoonotic potential in the pirarucu Arapaima gigas (Osteichthyes: Arapaimidae) from South America.

    Science.gov (United States)

    Andrade-Porto, S M; Cárdenas, M Q; Martins, M L; Oliveira, J K Q; Pereira, J N; Araújo, C S O; Malta, J C O

    2015-11-01

    Third-stage larvae (L3) of Hysterothylacium sp. were collected by the first time in juveniles of pirarucu Arapaima gigas farmed in the Rio Preto da Eva, Amazonas state. Ninety-eight (98) out of 100 examined fish showed to be parasitized. Five hundred and ninety larvae of Hysterothylacium sp. were collected from the intestines, stomach and pyloric caeca. The mean intensity of parasite indexes was 6.02 (±5.75) ranging from 1 to 40 larvae per host and the mean abundance was 5.9 (±5.76). The A. gigas is the new host record for larvae of Hysterothylacium sp. in Brazil, and this is the first record of larvae of Hysterothylacium (Nematoda: Anisakidae) with zoonotic potential in the pirarucu from South America.

  9. In vitro and in vivo anthelmintic effects of Caesalpinia bonducella (L. Roxb. leaf extract on Hymenolepis diminuta (Cestoda and Syphacia obvelata (Nematoda

    Directory of Open Access Journals (Sweden)

    Shyamalima Gogoi

    2016-12-01

    Full Text Available Background: Leaves of Caesalpinia bonducella (L. Roxb., have been traditionally used as herbal remedy against intestinal helminths helminth infections in traditional medicine of India. Aim: This study was undertaken to evaluate the potential in vitro and in vivo anthelmintic effects of C. bonducella leaf extract against Syphacia obvelata (Nematoda and Hymenolepis diminuta (Cestoda. Materials and Methods: The in vitro anthelmintic activity of the extract was investigated on adult Syphacia obvelata (Nematoda and Hymenolepis diminuta (Cestoda worms in terms of physical motility and mortality of parasites. The in vivo study was performed in H. diminuta-rat model and S. obvelata-mice model, by monitoring the egg per gram (EPG of faeces count and worm count of animals following the treatment with different doses of plant extract. Results: The study recorded significant and dose-dependent anthelmintic effects of extract on both the parasites. In the in vitro study, 30 mg/ml concentration of extract caused mortality of H. diminuta in 2.5±0.2 h and S. obvelata in 3.57± 0.16 h. In the in vivo study, the extract showed a comparatively better efficacy on S. obvelata, where its 800 mg/kg dose revealed 93% reduction of worm load in mice, as compared to 85% reduction of H. diminuta worms in load. Conclusions: The findings suggest that leaf extract of C. bonducella possesses significant anthelmintic effects, and supports its use as an anthelmintic in traditional medicine. This appears to be the first report of in vivo anthelmintic activity of Caesalpinia bonducella against these parasites [J Complement Med Res 2016; 5(4.000: 427-433

  10. Molecular diversity of bacterial endosymbionts associated with dagger nematodes of the genus Xiphinema (Nematoda: Longidoridae) reveals a high degree of phylogenetic congruence with their host.

    Science.gov (United States)

    Palomares-Rius, Juan E; Archidona-Yuste, Antonio; Cantalapiedra-Navarrete, Carolina; Prieto, Pilar; Castillo, Pablo

    2016-12-01

    Bacterial endosymbionts have been detected in some groups of plant-parasitic nematodes, but few cases have been reported compared to other groups in the phylum Nematoda, such as animal-parasitic or free-living nematodes. This study was performed on a wide variety of plant-parasitic nematode families and species from different host plants and nematode populations. A total of 124 nematode populations (previously identified morphologically and molecularly) were screened for the presence of potential bacterial endosymbionts using the partial 16S rRNA gene and fluorescence in situ hybridization (FISH) and confocal microscopy. Potential bacterial endosymbionts were only detected in nematode species belonging to the genus Xiphinema and specifically in the X. americanum group. Fifty-seven partial 16S rRNA sequences were obtained from bacterial endosymbionts in this study. One group of sequences was closely related to the genus 'Candidatus Xiphinematobacter' (19 bacterial endosymbiont sequences were associated with seven nematode host species, including two that have already been described and three unknown bacterial endosymbionts). The second bacterial endosymbiont group (38 bacterial endosymbiont sequences associated with six nematode species) was related to the family Burkholderiaceae, which includes fungal and soil-plant bacterial endosymbionts. These endosymbionts were reported for the first time in the phylum Nematoda. Our findings suggest that there is a highly specific symbiotic relationship between nematode host and bacterial endosymbionts. Overall, these results were corroborated by a phylogeny of nematode host and bacterial endosymbionts that suggested that there was a high degree of phylogenetic congruence and long-term evolutionary persistence between hosts and endosymbionts. © 2016 John Wiley & Sons Ltd.

  11. Aspects of the life history of Muspicea borreli (Nematoda: Muspiceidae, parasite of the house mouse (Mus domesticus in Australia

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    Spratt D.M.

    2002-09-01

    Full Text Available Prevalence of Muspicea borreli (Nematoda infection in wild populations of Mus domesticus in forests in southeastern New South Wales and in rural Canberra, Australia was variable, relatively low and the parasite occurred predominantly in male mice. Experimental infection of BALB/c mice occurred only via subcutaneous inoculation but was achieved using i adults containing embryonating eggs, ii adults containing active larvae and iii active larvae dissected from the uterus of female worms. Experimental infection was not established using adults containing unembryonated eggs and was not established via intraperitoneal, percutaneous nor oral routes. Evidence indicates that larvae develop to the infective stage in the uterus of the adult worm, suggests that an obligate developmental phase on the host skin does not occur and that autoinfection is possible. Experimental infection predominated in males; females rarely became infected. When male BALB/c mice were inoculated subcutaneously with M. borreli, immediately paired with an uninoculated female and permitted to breed for 90 days, infection was found in male and female offspring only of the second and subsequent litters or in the breeding female partner. Transmission to the young occurred within 21 days of birth and fifth-stage M. borreli were found in offspring of the second and subsequent litters only after 35 or more days. However, when a male was inoculated but mating delayed for 23 days, infection was found in progeny of the first and second litters. The life cycle is direct and the prepatent period in BALB/c mice is estimated at 50-60 days. The precise mode of transmission of the parasite in breeding pairs of mice was not determined but larvae remained active for approximately an hour in balanced saline solutions (pH = 7.2 and in human saliva but died under conditions emulating free-living (tap water pH = 7.1 and stomach (pepsin solution pH = 2 environments. Transmission was not effected by

  12. Camallanus maculatus n. sp. (Nematoda: Camallanidae in an ornamental fish Xiphophorus maculatus (Osteichthyes: Poeciliidae cultivated in São Paulo State, Brazil Camallanus maculatus n. sp. (Nematoda: Camallanidae em peixe ornamental Xiphophorus maculatus (Osteichthyes: Poeciliidae cultivado no estado de São Paulo, Brasil

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    M.L. Martins

    2007-10-01

    Full Text Available The present study describes Camallanus maculatus n. sp. (Nematoda: Camallanidae from Xiphophorus maculatus (Osteichthyes: Poecilidae collected in an ornamental fish farm in São Paulo State, Brazil. Thirty-three out of 40 examined fish were parasitized (82% prevalence with mean intensity of 2.8 (1-20. Male specimens were 3,500µm total length and females 7,100µm total length, provided with orange-brown buccal capsule and two developed tridents. Males showed two unequal spicules, gubernaculum absent and females with vulva situated in the middle of the body. The main characteristics were the sleekly longitudinal ridges in the buccal capsule in both sexes, enlargement at the anterior end of the smaller spicule, the number of genital papillae, longer muscular and glandular portion of esophagus and round tail in female.Descreve-se Camallanus maculatus n. sp. (Nematoda: Camallanidae em Xiphophorus maculatus (Osteichthyes: Poecilidae capturado em uma piscicultura ornamental no estado de São Paulo. De 40 peixes examinados, 33 estavam parasitados (prevalência de 82% cuja intensidade média foi de 2,8 (1-20. Os machos apresentaram 3.500µm e as fêmeas com 7,100µm de comprimento total, providos de uma cápsula bucal laranja-castanho e dois tridentes. Observaram-se machos com espículos desiguais, gubernáculo ausente e fêmeas com vulva situada na metade do corpo. As principais características foram as estrias longitudinais lisas em cada valva da cápsula bucal em ambos os sexos, o espículo menor com alargamento na extremidade anterior, o número de papilas genitais, as porções muscular e glandular do esôfago longas e a cauda longa e arredondada na fêmea.

  13. Rarely reported, widely distributed, and unexpectedly diverse: molecular characterization of mermithid nematodes (Nematoda: Mermithidae) infecting bumble bees (Hymenoptera: Apidae: Bombus) in the USA.

    Science.gov (United States)

    Tripodi, Amber D; Strange, James P

    2018-03-16

    Mermithid nematodes (Nematoda: Mermithida: Mermithidae) parasitize a wide range of both terrestrial and aquatic invertebrate hosts, yet are recorded in bumble bees (Insecta: Hymenoptera: Apidae: Bombus) only six times historically. Little is known about the specific identity of these parasites. In a single-season nationwide survey of internal parasites of 3646 bumble bees, we encountered six additional instances of mermithid parasitism in four bumble bee species and genetically characterized them using two regions of 18S to identify the specific host-parasite relationships. Three samples from the northeastern USA are morphologically and genetically identified as Mermis nigrescens, whereas three specimens collected from a single agricultural locality in the southeast USA fell into a clade with currently undescribed species. Nucleotide sequences of the V2-V6 region of 18S from the southeastern specimens were 2.6-3.0% divergent from one another, and 2.2-4.0% dissimilar to the nearest matches to available data. The dearth of available data prohibits positive identification of this parasite and its affinity for specific bumble bee hosts. By doubling the records of mermithid parasitism of bumble bee hosts and providing genetic data, this work will inform future investigations of this rare phenomenon.

  14. Historical biogeography among species of Varestrongylus lungworms (Nematoda: Protostrongylidae) in ungulates: episodic expansion and host colonization linking Eurasia and North America.

    Science.gov (United States)

    Verocai, Guilherme G; Kutz, Susan J; Hoberg, Eric P

    2018-05-03

    Varestrongylus lungworms (Nematoda: Protostrongylidae) include 10 nominal species that parasitize wild and domesticated artiodactyles. Eight species are endemic to the western Palearctic and Eurasia, whereas two are limited in distribution to the Nearctic. Complex host associations, primarily among Cervidae and Bovidae (Caprinae), and biogeography were explored based on direct comparisons of parasite and host phylogenies to reveal the historical development of this fauna. Diversification among Varestrongylus species has an intricate history extending over the Pliocene and Quaternary involving episodic processes for geographic and host colonization: (1) Varestrongylus has origins in Eurasia with independent expansion events into bordering ecozones; (2) cervids are ancestral hosts; (3) the caprine-associated V. pneumonicus is basal and a result of an independent host colonization event; (4) secondary diversification, linked to sequential and independent host colonization events, occurred within cervids (V. sagittatus + V. tuvae; V. alpenae; and V. capreoli, V. alces + V. eleguneniensis); (5) at least two additional host colonization events into caprines occurred, followed or not by diversification (V. qinghaiensis + V. longispiculatus; V. capricola, respectively); (6) two independent events of geographic expansion into North America from Eurasia with cervids in the late Pliocene and early Pleistocene are postulated (V. alpenae, V. eleguneniensis). Comparisons based on phylogenetic hypotheses derived from comparative morphology and molecular inference for these nematodes are consistent with the postulated history for coevolutionary and biogeographic history. Episodes of geographic and host colonization, often in relation to rapid shifts in climate and habitat perturbation, have dominated the history of diversification of Varestrongylus.

  15. New records of philometrids (Nematoda: Philometridae) from marine fishes off Australia, including description of four new species and erection of Digitiphilometroides gen. n.

    Science.gov (United States)

    Moravec, Frantisek; Barton, Diane P

    2018-04-16

    The following six species of the Philometridae (Nematoda: Dracunculoidea) were recorded from marine fishes off the northern coast of Australia in 2015 and 2016: Philometra arafurensis sp. n. and Philometra papillicaudata sp. n. from the ovary and the tissue behind the gills, respectively, of the emperor red snapper Lutjanus sebae (Cuvier); Philometra mawsonae sp. n. and Dentiphilometra malabarici sp. n. from the ovary and the tissue behind the gills, respectively, of the Malabar blood snapper Lutjanus malabaricus (Bloch et Schneider); Philometra sp. from the ovary of the goldbanded jobfish Pristipomoides multidens (Day) (Perciformes: all Lutjanidae); and Digitiphilometroides marinus (Moravec et de Buron, 2009) comb. n. from the body cavity of the cobia Rachycentron canadum (Linnaeus) (Perciformes: Rachycentridae). Digitiphilometroides gen. n. is established based on the presence of unique digital cuticular ornamentations on the female body. New gonad-infecting species, P. arafurensis and P. mawsonae, are characterised mainly by the length of spicules (252-264 µm and 351-435 µm, respectively) and the structure of the gubernaculum, whereas P. papillicaudata is characterised mainly by the body length (70 mm) of gravid female, extent of the oesophageal gland, size of caudal projections and the location in the host. Dentiphilometra malabarici differs from congeners mainly in the arrangement of circumoral teeth (in a single row), extent of the oesophageal gland and the absence of sclerotised teeth or protuberances on the oesophageal lobes in the mouth. Digitiphilometroides marinus has not previously been reported from fishes in Australian waters.

  16. Nematoda: Haliplectidae, Suborder Leptolaimina

    African Journals Online (AJOL)

    1991-09-13

    Sep 13, 1991 ... The Alexandria dune field lies between the Sundays River ... field may have originated in an area where the channel of an ... A: Anterior region of holotype male; B: Anterior region of female; C: External morphology of.

  17. Unravelling the Biodiversity and Molecular Phylogeny of Needle Nematodes of the Genus Longidorus (Nematoda: Longidoridae) in Olive and a Description of Six New Species.

    Science.gov (United States)

    Archidona-Yuste, Antonio; Navas-Cortés, Juan A; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan E; Castillo, Pablo

    2016-01-01

    The genus Longidorus includes a remarkable group of invertebrate animals of the phylum Nematoda comprising polyphagous root-ectoparasites of numerous plants including several agricultural crops and trees. Damage is caused by direct feeding on root cells as well as by transmitting nepoviruses that cause disease on those crops. Thus, correct identification of Longidorus species is essential to establish appropriate control measures. We provide the first detailed information on the diversity and distribution of Longidorus species infesting wild and cultivated olive soils in a wide-region in southern Spain that included 159 locations from which 449 sampling sites were analyzed. The present study doubles the known biodiversity of Longidorus species identified in olives by including six new species (Longidorus indalus sp. nov., Longidorus macrodorus sp. nov., Longidorus onubensis sp. nov., Longidorus silvestris sp. nov., Longidorus vallensis sp. nov., and Longidorus wicuolea sp. nov.), two new records for wild and cultivate olives (L. alvegus and L. vineacola), and two additional new records for wild olive (L. intermedius and L. lusitanicus). We also found evidence of some geographic species associations to western (viz. L. alvegus, L. intermedius, L. lusitanicus, L. onubensis sp. nov., L. vineacola, L. vinearum, L. wicuolea sp. nov.) and eastern distributions (viz. L. indalus sp. nov.), while only L. magnus was detected in both areas. We developed a comparative study by considering morphological and morphometrical features together with molecular data from nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S, ITS1, and partial 18S). Results of molecular and phylogenetic analyses confirmed the morphological hypotheses and allowed the delimitation and discrimination of six new species of the genus described herein and four known species. Phylogenetic analyses of Longidorus spp. based on three molecular markers resulted in a general consensus of these species

  18. Larvas de simulídeos (Diptera, Simuliidae do centro oeste, sudeste e sul do Brasil, parasitadas por microsporídeos (Protozoa e mermitídeos (Nematoda Simulids larvae (Diptera, Simuliidae from middle western, southeastern and southern Brazil, with microsporids (Protozoa and mermithids (Nematoda parasites

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    Carmen Ambrós Ginarte

    2003-09-01

    Full Text Available A survey of simulid larval parasites was carried out in different localities of the states of Mato Grosso, Minas Gerais, São Paulo, Paraná, Santa Catarina and Rio Grande do Sul, Brazil, from February 1996 to May 1998. Prevalences for the microsporidian Polydispyrenia simulii Lutz & Splendore, 1908 were found in Morungaba and Leme, São Paulo, ranging from around 0.7 to 66.7%, depending mainly on the host simulid species. Microsporidiosis was registered in localities of São Paulo, Paraná, Santa Catarina and Rio Grande do Sul. Parasitism by Isomermis sp. (Nematoda, Mermithidae was found in Simulium larvae from Serra do Japi, ranging from 0.8 to 45.8%, depending on the simulid species and the larval microhabitat in the stream, whether a cemented ramp in a lake outlet or the natural stream bed. Parasitism by mermithids was also found in ten localities. Mycoses caused by Coelomycidium sp. were for the first time recorded for larvae of Simulium (Chirostilbia pertinax Kollar, 1832.

  19. Type material of Acanthocephala, Nematoda and other non-helminths phyla (Cnidaria, Annelida, and Arthropoda housed in the Helminthological Collection of the Oswaldo Cruz Institute/ FIOCRUZ (CHIOC, Rio de Janeiro, Brazil, from 1979 to 2016

    Directory of Open Access Journals (Sweden)

    Daniela A. Lopes

    2017-10-01

    Full Text Available The third part of the catalogue of type material in the Helminthological Collection of the Oswaldo Cruz Institute/FIOCRUZ (CHIOC, comprising types deposited between 1979 and 2016, is presented to complement the first list of all types that was published in 1979. This part encompasses Acanthocephala, Nematoda and the other non-helminth phyla Cnidaria, Annelida, and Arthropoda. Platyhelminthes was covered in the first (Monogenoidea and second (Rhabditophora Trematoda and Cestoda parts of the catalogue published in September 2016 and March 2017, respectively. The present catalogue comprises type material for 116 species distributed across five phyla, nine classes, 50 families, and 80 genera. Specific names are listed systematically, followed by type host, infection site, type locality, and specimens with their collection numbers and references. Species classification and nomenclature are updated.

  20. Serine protease inhibitors of parasitic helminths.

    Science.gov (United States)

    Molehin, Adebayo J; Gobert, Geoffrey N; McManus, Donald P

    2012-05-01

    Serine protease inhibitors (serpins) are a superfamily of structurally conserved proteins that inhibit serine proteases and play key physiological roles in numerous biological systems such as blood coagulation, complement activation and inflammation. A number of serpins have now been identified in parasitic helminths with putative involvement in immune regulation and in parasite survival through interference with the host immune response. This review describes the serpins and smapins (small serine protease inhibitors) that have been identified in Ascaris spp., Brugia malayi, Ancylostoma caninum Onchocerca volvulus, Haemonchus contortus, Trichinella spiralis, Trichostrongylus vitrinus, Anisakis simplex, Trichuris suis, Schistosoma spp., Clonorchis sinensis, Paragonimus westermani and Echinococcus spp. and discusses their possible biological functions, including roles in host-parasite interplay and their evolutionary relationships.

  1. First description of gastrointestinal nematodes of Barbary sheep (Ammotragus lervia): the case of Camelostrongylus mentulatus as a paradigm of phylogenic and specific relationship between the parasite and its ancient host.

    Science.gov (United States)

    Mayo, E; Ortiz, J; Martínez-Carrasco, C; Garijo, M M; Espeso, G; Hervías, S; Ruiz de Ybáñez, M R

    2013-09-01

    The gastrointestinal helminth fauna of 24 Barbary sheep or Aoudad (Ammotragus lervia sahariensis) maintained in the Parque de Rescate de la Fauna Sahariana (PRFS, CSIC, Almeria, Spain) was analyzed. Most animals (87.5 %) were parasitized, and multiple infections were highly present. The following species were identified: Camelostrongylus mentulatus, Teladorsagia circumcincta, Marshallagia marshalli, Ostertagia ostertagi, O. leptospicularis, O. lyrata, Haemonchus contortus, Teladorsagia trifurcata, Trichostrongylus vitrinus, T. colubriformis, T. probolorus, T. capricola, Nematodirus spathiger, N. abnormalis, N. filicollis, N. helvetianus, Trichuris spp. and Skrjabinema ovis. Teladorsagia circumcincta was the most prevalent nematode in abomasum (52.6 %) followed by C. mentulatus (50 %). However, this latter nematode had the greater mean intensity and abundance. In the small intestine, T. colubriformis and T. vitrinus had the highest prevalence (36.4 %); the last one showed also the greater mean intensity and abundance. It should be emphasized the presence of Skrjabinema ovis (prevalence 39.1 %) in the large intestine, showing the greater mean abundance and intensity, although with a low values. Camelostrongylus mentulatus could be the most primitive nematode of the family trichostrongylidae recovered in this study; attending to its high prevalence, mean abundance and mean intensity, the possible specificity between this parasite and the Aoudad is discussed.

  2. Halophilanema prolata n. gen., n. sp. (Nematoda: Allantonematidae, a parasite of the intertidal bug, Saldula laticollis (Reuter(Hemiptera: Saldidae on the Oregon coast

    Directory of Open Access Journals (Sweden)

    Poinar George O

    2012-02-01

    Full Text Available Abstract Background It is rare to find terrestrial nematode lineages parasitizing arthropods inhabiting the intertidal or littoral zone of the oceans. During an ecological study along the Oregon dunes, an allantonematid nematode (Tylenchomorpha: Allantonematidae was discovered parasitizing the intertidal shore bug, Saldula laticollis (Reuter(Hemiptera: Saldidae. This shore bug is adapted to an intertidal environment and can survive short periods of submergence during high tides. The present study describes the nematode parasite and discusses aspects of its development, ecology and evolution. Methods Adults and last instar nymphs of S. laticollis (Hemiptera: Saldidae were collected from the high intertidal zone among clumps of Juncus L. (Juncaceae plants at Waldport, Oregon on October 3, 2011. The bugs were dissected in 1% saline solution and the nematodes killed in 1% Ringers solution and immediately fixed in 5% formalin (at 20°C. Third stage juveniles removed from infected hosts were maintained in 1% saline solution until they matured to the adult stage, molted and mated. Results Halophilanema prolata n. gen., n. sp. (Nematoda: Allantonematidae is described from last instar nymphs and adults of the intertidal bug, Saldula laticollis on the Oregon coast. The new genus can be distinguished from other genera in the Allantonematidae by a stylet lacking basal knobs in both sexes, an excretory pore located behind the nerve ring, ribbed spicules, a gubernaculum, the absence of a bursa and the elongate-tubular shape of the ovoviviparous parasitic females. Studies of the organogenesis of Halophilanema showed development to third stage juveniles in the uterus of parasitic females. Maturation to the free-living adults and mating occurred in the environment. The incidence of infection of S. laticollis ranged from 0% to 85% depending on the microhabitat in the intertidal zone. Conclusions Based on the habitat and morphological characters, it is proposed

  3. Remarkable Diversity and Prevalence of Dagger Nematodes of the Genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Olives Revealed by Integrative Approaches

    Science.gov (United States)

    Archidona-Yuste, Antonio; Navas-Cortés, Juan A.; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan E.

    2016-01-01

    The genus Xiphinema includes a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of Xiphinema species is critical for launching appropriate control measures. We make available the first detailed information on the diversity and distribution of Xiphinema species infesting wild and cultivated olive in a wide-region in southern Spain that included 211 locations from which 453 sampling sites were analyzed. The present study identified thirty-two Xiphinema spp. in the rhizosphere of olive trees, ten species belonging to Xiphinema americanum-group, whereas twenty-two were attributed to Xiphinema non-americanum-group. These results increase our current knowledge on the biodiversity of Xiphinema species identified in olives and include the description of four new species (Xiphinema andalusiense sp. nov., Xiphinema celtiense sp. nov., Xiphinema iznajarense sp. nov., and Xiphinema mengibarense sp. nov.), and two new records for cultivate olives (X. cadavalense and X. conurum). We also found evidence of remarkable prevalence of Xiphinema spp. in olive trees, viz. 85.0% (385 out of 453 sampling sites), and they were widely distributed in both wild and cultivated olives, with 26 and 17 Xiphinema spp., respectively. Diversity indexes (Richness, Hill´s diversity, Hill´s reciprocal of D and Hill´s evenness) were significantly affected by olive type. We also developed a comparative morphological and morphometrical study together with molecular data from three nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S, ITS1, and partial 18S). Molecular characterization and phylogenetic analyses allowed the delimitation and discrimination of four new species of the genus described herein and three known species. Phylogenetic analyses of Xiphinema spp. resulted in a

  4. Remarkable Diversity and Prevalence of Dagger Nematodes of the Genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Olives Revealed by Integrative Approaches.

    Science.gov (United States)

    Archidona-Yuste, Antonio; Navas-Cortés, Juan A; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan E; Castillo, Pablo

    2016-01-01

    The genus Xiphinema includes a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of Xiphinema species is critical for launching appropriate control measures. We make available the first detailed information on the diversity and distribution of Xiphinema species infesting wild and cultivated olive in a wide-region in southern Spain that included 211 locations from which 453 sampling sites were analyzed. The present study identified thirty-two Xiphinema spp. in the rhizosphere of olive trees, ten species belonging to Xiphinema americanum-group, whereas twenty-two were attributed to Xiphinema non-americanum-group. These results increase our current knowledge on the biodiversity of Xiphinema species identified in olives and include the description of four new species (Xiphinema andalusiense sp. nov., Xiphinema celtiense sp. nov., Xiphinema iznajarense sp. nov., and Xiphinema mengibarense sp. nov.), and two new records for cultivate olives (X. cadavalense and X. conurum). We also found evidence of remarkable prevalence of Xiphinema spp. in olive trees, viz. 85.0% (385 out of 453 sampling sites), and they were widely distributed in both wild and cultivated olives, with 26 and 17 Xiphinema spp., respectively. Diversity indexes (Richness, Hill´s diversity, Hill´s reciprocal of D and Hill´s evenness) were significantly affected by olive type. We also developed a comparative morphological and morphometrical study together with molecular data from three nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S, ITS1, and partial 18S). Molecular characterization and phylogenetic analyses allowed the delimitation and discrimination of four new species of the genus described herein and three known species. Phylogenetic analyses of Xiphinema spp. resulted in a

  5. Discovery of new Ohbayashinema spp. (Nematoda: Heligmosomoidea) in Ochotona princeps and Ochotona cansus (Lagomorpha: Ochotonidae) from western North America and Central Asia, with considerations of historical biogeography.

    Science.gov (United States)

    Durette-Desset, M-C; Galbreath, K E; Hoberg, E P

    2010-06-01

    Three new species of Ohbayashinema (Nematoda, Heligmosomoidea) are described from localities in western North America and central Asia. Two of these species, Ohbayashinema nearctica n. sp. and Ohbayashinema aspeira n. sp., are parasitic in American pika, Ochotona princeps. Ohbayashinema nearctica is differentiated from the 5 known species of the genus parasitic in Ochotonidae from the Old World by very long spicules and an oblique axis of orientation for the ridges composing the synlophe. Ohbayashinema aspeira, described only from females, is similar to Oh. nearctica based on the number of cuticular ridges at the mid-body. It is mainly differentiated by an uncoiled anterior extremity and by near equal dimensions of the vestibule and the uterus. The third species, Ohbayashinema patriciae n. sp., is parasitic in Gansu pika, Ochotona cansus , from China. It is similar to Ohbayashinema erbaevae parasitic in Ochotona dauurica from Buriatia and Ohbayashinema ochotoni in Ochotona macrotis from Nepal, based on the length of the spicules and the ratio of spicule length to body length. It differs from the former species by possessing a smaller number of cuticular ridges and in the comparative length of the vestibule and infundibulum. Related to Oh. ochotoni by an identical number of cuticular ridges at the mid-body, it differs from this species in having smaller ridges in the dorsal rather than ventral field and in the dimensions of the dorsal ray where rays 9 are less than rays 10. Species of Ohbayashinema appear to be host-specific among the Ochotonidae but had not been previously reported in pikas from the Nearctic. Although much remains to be demonstrated about the diversity for helminths in pikas, it is apparent that factors associated with the assembly and structure of parasite faunas have been complex, involving episodic processes for geographic and host colonization along with coevolutionary mechanisms. Understanding the historical factors, particularly climate

  6. Anisakid nematodes (Nematoda: Anisakidae) from the marine fishes Plectropomus laevis Lacépède (Serranidae) and Sphyraena qenie Klunzinger (Sphyraenidae) off New Caledonia, including two new species of Hysterothylacium Ward & Magath, 1917.

    Science.gov (United States)

    Moravec, František; Justine, Jean-Lou

    2015-11-01

    Based on light and scanning electron microscopical studies, two new species of Hysterothylacium Ward & Magath, 1917 (Nematoda: Anisakidae) are described from the digestive tract of perciform fishes off New Caledonia: H. alatum n. sp. from Plectropomus laevis (Lacépède) (Serranidae) and H. sphyraenae n. sp. from Sphyraena qenie Klunzinger (Sphyraenidae). The former species (H. alatum) is mainly characterised by its large body (male 42.05 mm, gravid females 51.18-87.38 mm long), the shape of the dorsal lip, conspicuously broad cervical alae, a short caecum and a long ventricular appendix, the length of the spicules (925 µm), the number (25 pairs) and distribution of the genital papillae and the tail tip bearing numerous minute cuticular protuberances. The other species (H. sphyraenae) is mainly characterised by the presence of narrow lateral alae, a short caecum and a long ventricular appendix, the length (762-830 µm) and shape of the spicules, the number (37-38 pairs) and arrangement of the genital papillae, and by the tail tip which lacks any distinct cuticular projections visible under the light microscope. In addition, and unidentifiable at the species level, conspicuously large (45.71-66.10 mm long) larvae of Contracaecum Railliet & Henry, 1912, were found in the body cavity of P. laevis, which serves as a paratenic host for this parasite.

  7. Metazoan-protozoan parasite co-infections and host body weight in St Kilda Soay sheep.

    Science.gov (United States)

    Craig, B H; Tempest, L J; Pilkington, J G; Pemberton, J M

    2008-04-01

    For hundreds of years, the unmanaged Soay sheep population on St Kilda has survived despite enduring presumably deleterious co-infections of helminth, protozoan and arthropod parasites and intermittent periods of starvation. Important parasite taxa in young Soay sheep are strongyles (Trichostrongylus axei, Trichostrongylus vitrinus and Teladorsagia circumcincta), coccidia (11 Eimeria species) and keds (Melophagus ovinus) and in older animals, Teladorsagia circumcincta. In this research, associations between the intensity of different parasite taxa were investigated. Secondly, the intensities of different parasite taxa were tested for associations with variation in host weight, which is itself a determinant of over-winter survival in the host population. In lambs, the intensity of strongyle eggs was positively correlated with that of Nematodirus spp. eggs, while in yearlings and adults strongyle eggs and coccidia oocysts were positively correlated. In lambs and yearlings, of the parasite taxa tested, only strongyle eggs were significantly and negatively associated with host weight. However, in adult hosts, strongyles and coccidia were independently and negatively associated with host weight. These results are consistent with the idea that strongyles and coccidia are exerting independent selection on Soay sheep.

  8. Species of Philometra (Nematoda, Philometridae from fishes off the Mediterranean coast of Africa, with a description of Philometra rara n. sp. from Hyporthodus haifensis and a molecular analysis of Philometra saltatrix from Pomatomus saltatrix

    Directory of Open Access Journals (Sweden)

    Moravec František

    2017-01-01

    Full Text Available Two gonad-infecting species of Philometra Costa, 1845 (Nematoda, Philometridae were recorded for the first time from marine perciform fishes off Tunisia and Libya: Philometra rara n. sp. from the rare, deep-water Haifa grouper Hyporthodus haifensis (Serranidae off Libya and Philometra saltatrix Ramachandran, 1973 from the bluefish Pomatomus saltatrix (Pomatomidae off Tunisia. Identification of both fish species was confirmed by molecular barcoding. Light and scanning electron microscope studies of Ph. rara n. sp. showed that it is characterized by the length of spicules (216–219 μm and the gubernaculum (90–93 μm, the gubernaculum/spicules length ratio (1:2.32–2.43, and mainly by the shape and structure of the distal end of the gubernaculum (shovel-shaped with a wide median smooth field in dorsal view, appearing as having a dorsal protuberance in lateral view, and by the structure of the male caudal mound (dorsally interrupted; large subgravid females (70–137 mm long are characterized by the presence of four oval submedian cephalic elevations, each of them bearing a pair of cephalic papillae of the outer circle. The finding of Ph. saltatrix off Tunisia confirms that this species is widespread throughout the Mediterranean region. A molecular analysis of our Ph. saltatrix specimens and other available philometrid cytochrome c oxidase 1 (COI sequences showed that most species have robust clades. Sequences of Ph. saltatrix from Tunisia diverge from Ph. saltatrix from Brazil and the USA, suggesting that speciation is currently occurring between populations from both sides of the Atlantic Ocean.

  9. The effect of sire selection on the response of lambs to vaccination with irradiated Trichostrongylus colubriformis larvae

    International Nuclear Information System (INIS)

    Dineen, J.K.; Windon, R.G.

    1980-01-01

    Rams selected for responsiveness and unresponsiveness to vaccination with irradiated T. colubriformis larvae at an early age were mated to unselected random bred ewes. Progeny were vaccinated with 20,000 irradiated larvae at 8 and 12 weeks of age, given anthelmintic treatment at 16 weeks and challenged with 20,000 normal larvae at 17 weeks. The results, based on wether worm counts and ewe faecal egg counts, showed significant differences between responder and non-responder progeny. There was a significant correlation between worm counts and faecal egg counts of half-sibs from the same sire group. The occurrence of globule leucocytes was inversely related to worm burdens of wether progeny, however, no clear relationship was found with eosinophils. In vitro lymphocyte stimulation using T. colubriformis L 3 antigen, concanavalin A and lipopolysaccharide showed that statistically defined responder progeny, pooled from both responder and non-responder sire groups, gave higher responses than non-responder lambs after vaccination. The results confirm that genetically-determined factors are involved in the response of lambs to vaccination at an early age, and indicate that rapid genetic progress may be achieved in the type of mating usually carried out under field conditions. (author)

  10. Anthelmintic-resistant strains of Haemonchus contortus and Trichostrongylus colubriformis isolated from an organic sheep and goat farm in Denmark

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Enemark, Heidi L.; Thansborg, Stig M.

    2013-01-01

    A suspected case of anthelmintic resistance (AR) was investigated in an organic dairy sheep and goat farm. The herd was established in 2007 by purchase of animals from a number of other farms. Selection for the study was based on history of anthelmintic-treatment failure. Forty-eight lambs and 48......) or 10 mg/kg closantel (CLO). Kids were treated with 10 mg/kg FBZ, 0.3 mg/kg MOX, 14 mg/kg LEV, 0.2 mg/kg IVM or 10 mg/kg CLO. FECs were performed at day of treatment and 14 days post treatment. In a subsequent investigation, faeces from adult goats were cultured to obtain 3rd-stage nematode larvae (L3...... %. This is the first isolation of BZ-resistant H. contortus and T. colubriformis in Denmark and highlights the need for continuous surveillance of AR in conventional and organic farms....

  11. Performans Produksi, Jumlah Nematoda Usus, dan Profil Metabolik Darah Kambing yang Diberi Pakan Hijauan Rawa Kalimantan (PRODUCTION PERFORMANS, INTESTINE NEMATODE NUMBER AND METABOLIC BLOOD PROFILE OF GOAT FEED WITH BORNEO SWAMP FORAGE

    Directory of Open Access Journals (Sweden)

    Tintin Rostini

    2017-09-01

    Full Text Available This study aims were to determine the effect of the use of swamp forage on the performances, the number of nematodes and the blood metabolic profile in goats. In this study, as many as 24 male goats were used; consisted of 12 local male goats (kacang goat with the average weight ranged from 12.65±1.65kg (diversity coefficient 11.34% and 12 PE goats with the average weight of 18.05±0.62 kg (diversity coefficient 7.54%. This research used a completely randomized factorial design (CRD 2x4 with 2 main treatments x 4 factorials and three replications. The first factor was based on the goat breed being used (Kacang and Peranakan Etawah. The second factor was based on four differents feed percentages that used, i.e.: (PR0 40% grass forage and 60% concentrate, (PR1 60% grass forage and 40% concentrate, (PR2, 40% of swamp forage and concentrates 60%, (PR3 60% swamp forage and 40% concentrate. Data were analyzed by using analysis of variance, then continued with Duncan test. The results of this study showed that the consumption of nutrients, daily weight gain and feed efficiency in treatment provision of swamp forage up to 60% (PR3 gave best result based on the metabolic profile of blood (total protein, glucose, cholesterol, Ca and P. The number of worm eggs in the goat feces were still in normal range. It was concluded that swamp forage is a potential forage that can be used as an alternative towards grass forage for the goats. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan hijauan rawa terhadap performans, jumlah nematoda, dan profil metabolik darah kambing. Pada penelitian ini digunakan ternak kambing jantan sebanyak 24 ekor. Kambing yang digunakan terdiri dari 12 ekor kambing kacang jantan dengan rataan bobot badan berkisar antara 12,65 ± 1,65 kg (koefisien keragaman 11,34% dan 12 ekor kambing peranakan etawah (PE dengan rataan bobot badan 18,05 ± 0,62 kg (koefisien keragaman 7,54%. Dalam penelitian ini digunakan rancangan

  12. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia.

    Science.gov (United States)

    Sissay, Menkir M; Uggla, Arvid; Waller, Peter J

    2007-10-01

    A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger Oesopha-gostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the

  13. Feed intake and utilization by the parasitized ruminant

    International Nuclear Information System (INIS)

    Coop, R.L.

    1981-01-01

    Chronic abomasal parasitism was induced in sheep reared worm-free from birth by daily dosing with Ostertagia circumcincta larvae, and intestinal parasitism by either Trichostrongylus colubriformis or Trichostrongylus vitrinus larvae. The experimental design allowed effects on food intake to be separated from changes in efficiency of food utilization. The effects of the parasites were assessed from conventional balance trials and comparative slaughter techniques. Infection with gastrointestinal parasites severely limited animal performance in the absence of obvious clinical signs. Liveweight gain was reduced by 30-50%, partly as a result of reductions of 10-20% in voluntary food intake and partly from a decrease in the efficiency of utilization of food. Apparent digestibility of nitrogen was lowered by 25% in the early stages of Ostertagia infections but subsequently showed a recovery. Lower nitrogen retention was reflected in the decreased deposition of protein in the bodies of both O. and T. infected sheep. Fat deposition in the body was reduced by abomasal and intestinal parasitism as a result of reductions in food intake and a 30-50% reduction in the gross efficiency of utilization of metabolizable energy for growth. The rate of deposition of minerals in the skeleton was decreased by gastrointestinal parasitism and there is evidence that absorption of minerals from the intestine is impaired in T. colubriformis infections. The possible mechanisms responsible for the reductions in efficiency of utilization of nutrients by the parasitized ruminant are discussed. Only passing reference is made to the use of 32 P for calculating endogenous loss of P in sheep receiving 3000 T. colubriformis larvae per day

  14. Efficacy of an ivermectin controlled-release capsule against nematode and arthropod endoparasites in sheep.

    Science.gov (United States)

    Rehbein, S; Batty, A F; Barth, D; Visser, M; Timms, B J; Barrick, R A; Eagleson, J S

    1998-03-28

    Five controlled trials were conducted in Germany or in the United Kingdom, using 74 female sheep of merino or Dorset horn breeds, to evaluate the efficacy of an ivermectin controlled-release capsule against naturally acquired or induced infections of gastrointestinal nematodes, lungworms and nasal bot larvae and against incoming infections with gastrointestinal and pulmonary nematodes. Half of the animals were treated with one ivermectin controlled-release capsule that delivered ivermectin at the rate of 1.6 mg per day for 100 days while the other half remained untreated. Parasites were counted 21, 28, 35 or 56 days after administration of the capsule. The treatment was highly effective (> or = 99 per cent) against established parasites of the following species: Haemonchus contortus (adults and fourth-stage larvae), Ostertagia circumcincta, O pinnata, O trifurcata, Ostertagia species fourth-stage larvae, Trichostrongylus axei, T colubriformis, T vitrinus, Cooperia curticei, Nematodirus battus, N filicollis, Strongyloides papillosus, Chabertia ovina, Oesophagostomum venulosum, Trichuris ovis, Tr skrjabini, Dictyocaulus filaria, Protostrongylus rufescens and Oestrus ovis (larvae). The treatment prevented the establishment of the gastrointestinal nematodes H contortus, O circumcincta, T axei, T colubriformis, C curticei, N battus, N filicollis, Ch ovina, Oe vennulosum and the establishment of the lungworm D filaria by > 99 per cent compared with untreated controls (P < or = 0.01).

  15. The parasitic fauna of the European bison (Bison bonasus) (Linnaeus, 1758) and their impact on the conservation. Part 1. The summarising list of parasites noted.

    Science.gov (United States)

    Karbowiak, Grzegorz; Demiaszkiewicz, Aleksander W; Pyziel, Anna M; Wita, Irena; Moskwa, Bożena; Werszko, Joanna; Bień, Justyna; Goździk, Katarzyna; Lachowicz, Jacek; Cabaj, Władysław

    2014-09-01

    During the current century, 88 species of parasites have been recorded in Bison bonasus. These are 22 species of protozoa (Trypanosoma wrublewskii, T. theileri, Giardia sp., Sarcocystis cruzi, S. hirsuta, S. hominis, S. fusiformis, Neospora caninum, Toxoplasma gondii, Cryptosporidium sp., Eimeria cylindrica, E. subspherica, E. bovis, E. zuernii, E. canadensis, E. ellipsoidalis, E. alabamensis, E. bukidnonensis, E. auburnensis, E. pellita, E. brasiliensis, Babesia divergens), 4 trematodes species (Dicrocoelium dendriticum, Fasciola hepatica, Parafasciolopsis fasciolaemorpha, Paramphistomum cervi), 4 cestodes species (Taenia hydatigena larvae, Moniezia benedeni, M. expansa, Moniezia sp.), 43 nematodes species (Bunostomum trigonocephalum, B. phlebotomum, Chabertia ovina, Oesophagostomum radiatum, O. venulosum, Dictyocaulus filaria, D.viviparus, Nematodirella alcidis, Nematodirus europaeus, N. helvetianus, N. roscidus, N. filicollis, N. spathiger, Cooperia oncophora, C. pectinata, C. punctata, C. surnabada, Haemonchus contortus, Mazamastrongylus dagestanicus, Ostertagia lyrata, O. ostertagi, O. antipini, O. leptospicularis, O. kolchida, O. circumcincta, O. trifurcata, Spiculopteragia boehmi, S. mathevossiani, S. asymmetrica, Trichostrongylus axei, T. askivali, T. capricola, T. vitrinus, Ashworthius sidemi, Onchocerca lienalis, O. gutturosa, Setaria labiatopapillosa, Gongylonema pulchrum, Thelazia gulosa, T. skrjabini, T. rhodesi, Aonchotheca bilobata, Trichuris ovis), 7 mites (Demodex bisonianus, D. bovis, Demodex sp., Chorioptes bovis, Psoroptes equi, P. ovis, Sarcoptes scabiei), 4 Ixodidae ticks (Ixodes ricinus, I. persulcatus, I. hexagonus, Dermacentor reticulatus), 1 Mallophaga species (Bisonicola sedecimdecembrii), 1 Anoplura (Haematopinus eurysternus), and 2 Hippoboscidae flies (Lipoptena cervi, Melophagus ovinus). There are few monoxenous parasites, many typical for cattle and many newly acquired from Cervidae.

  16. ISOLASI NEMATODA PATOGEN SERANGGA STEINERNEMA DAN HETERORHABDITIS

    Directory of Open Access Journals (Sweden)

    Chaerani, Y. Suryadi, T.P. Priyatno, D. Koswanudin1, U. Rahmat , Sujatmo, Yusuf, dan C.T. Griffin.

    2012-02-01

    Full Text Available Isolation of Entomopathogenic Nematodes Steinernema and Heterorhabditis. Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis (Rhabditida: Steinernematidae and Heterorhabditidae are promising biological control agent of insect pests. Indigenous nematodes have been isolated and collected for the use in local biological control program of important insect pests. The nematodes were isolated using soil baiting method with insect larvae. Laboratory tests have shown that the mealworm larvae Tenebrio molitor (Coleoptera: Tenebrionidae served as a good alternative to the standard insect bait, the greater wax moth larvae Galleria mellonella (Lepidoptera: Galleriidae for isolation and maintenance of nematodes. Both nematodes were successfully isolated using T. molitor larvae from 13% soil samples (26 out of a total of 207 collected from 14 locations in West and Central Java and Lampung provinces in the period of 1993 until 2006. Heterorhabditis (9% was more prevalent than Steinernema (4%. Both nematodes were successfully propagated on mealworm larvae.

  17. Taxonomic study of entomopathogenic nematodes (Nematoda ...

    African Journals Online (AJOL)

    ... et d'hybridation effectuées utilisant les stades développementaux des nématodes ont révélé un isolat d'H. indica, deux d'H. sonorensis. Pour les autres isolats, des informations complémentaires sont nécessaires pour une identification complète. Le séquençage et l'analyse phylogénétique de la région interspécifique de ...

  18. from Capri vi, Namibia (Nematoda: Onchulinae)

    African Journals Online (AJOL)

    1992-10-01

    Oct 1, 1992 ... 1940). The species herein described does not fit into any of the existing genera, and we accordingly propose a new genus for its reception. Material and Methods. Descriptions, measurements and drawings were made from specimens killed and fixed in hot FAA (70°C) and mounted in anhydrous glycerine.

  19. Taxonomic study of entomopathogenic nematodes (Nematoda ...

    African Journals Online (AJOL)

    Le séquençage et l'analyse phylogénétique de la région interspécifique de l'ADN ribosomal ont groupé nos isolats avec H. sonorensis et H. Taysearae dans les arbres de parcimonie maximale et de Neighbour Joining avec les supports respectifs 94 et 99 %. Les caractères morphologiques des juvéniles infectieux et des ...

  20. A multiplex PCR-based method to identify strongylid parasite larvae recovered from ovine faecal cultures and/or pasture samples.

    Science.gov (United States)

    Bisset, S A; Knight, J S; Bouchet, C L G

    2014-02-24

    A multiplex PCR-based method was developed to overcome the limitations of microscopic examination as a means of identifying individual infective larvae from the wide range of strongylid parasite species commonly encountered in sheep in mixed sheep-cattle grazing situations in New Zealand. The strategy employed targets unique species-specific sequence markers in the second internal transcribed spacer (ITS-2) region of ribosomal DNA of the nematodes and utilises individual larval lysates as reaction templates. The basic assay involves two sets of reactions designed to target the ten strongylid species most often encountered in ovine faecal cultures under New Zealand conditions (viz. Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Cooperia curticei, Cooperia oncophora, Nematodirus spathiger, Chabertia ovina, and Oesophagostomum venulosum). Five species-specific primers, together with a pair of "generic" (conserved) primers, are used in each of the reactions. Two products are generally amplified, one by the generic primer pair regardless of species (providing a positive PCR control) and the other (whose size is indicative of the species present) by the appropriate species-specific primer in combination with one or other of the generic primers. If necessary, any larvae not identified by these reactions can subsequently be tested using primers designed specifically to detect those species less frequently encountered in ovine faecal cultures (viz. Ostertagia ostertagi, Ostertagia leptospicularis, Cooperia punctata, Nematodirus filicollis, and Bunostomum trigonocephalum). Results of assays undertaken on >5500 nematode larvae cultured from lambs on 16 different farms distributed throughout New Zealand indicated that positive identifications were initially obtained for 92.8% of them, while a further 4.4% of reactions gave a generic but no visible specific product and 2.8% gave no discernible

  1. Efficacy of a combined oral formulation of derquantel-abamectin against the adult and larval stages of nematodes in sheep, including anthelmintic-resistant strains.

    Science.gov (United States)

    Little, Peter R; Hodge, Andrew; Maeder, Steven J; Wirtherle, Nicole C; Nicholas, David R; Cox, George G; Conder, George A

    2011-09-27

    and respiratory nematode parasites of sheep, as follows: ≥ 98.9% efficacy against Haemonchus contortus (adult and L4); Teladorsagia circumcincta (adult, L4 and hypobiotic L4); Teladorsagia trifurcata (L4); Trichostrongylus axei (adult and L4); Trichostrongylus colubriformis (adult and L4); Trichostrongylus falculatus (adult); Trichostrongylus rugatus (adult); Trichostrongylus vitrinus (adult and L4); Cooperia curticei (adult and L4); Cooperia oncophora (adult and L4); Nematodirus spathiger (adult); Nematodirus battus (adult); Nematodirus spp. (hypobiotic L4); Strongyloides papillosus (adult); Strongyloides spp. (L4); Chabertia ovina (adult); Oesophagostomum venulosum (adult); Dictyocaulus filaria (adult); and Protostrongylus rufescens (adult); ≥ 97.0% efficacy against Trichuris ovis (adult); and ≥ 95.9% efficacy against T. trifurcata (adult). Derquantel-abamectin is a highly effective combination anthelmintic, which will provide an important new tool for controlling helminths of sheep when used in conjunction with sustainable drenching practices. Copyright © 2011 Elsevier B.V. All rights reserved.

  2. Halicephalobus gingivalis (Nematoda) infection in a Grevy's zebra (Equus grevyi).

    Science.gov (United States)

    Isaza, R; Schiller, C A; Stover, J; Smith, P J; Greiner, E C

    2000-03-01

    A 6-yr-old female Grevy's zebra (Equus grevyi) with a disseminated rhabditiform nematode infection is described. Antemortem clinical signs were limited to blindness and abnormal behavior believed to be caused by a recurrent nematode-induced uveitis. Histologic examination of the kidneys, heart, eyes, uterus, and lymph nodes revealed granulomas containing multiple sections of rhabditiform nematodes. Most of the recovered nematodes were larval stages with only a few adult females noted. The adults measured 243-297 microm x 11-16 microm (x = 269 x 14 microm). The distinctive rhabditiform esophagi had corpus:isthmus:bulb proportions of 19:11:5. On the basis of adult morphology, the nematode was identified as Halicephalobus gingivalis. This is the first report of this parasite in a zebra and indicates that this parasitic granulomatous disease should be considered in zebras with neurologic disease.

  3. The bipartite mitochondrial genome of Ruizia karukerae (Rhigonematomorpha, Nematoda).

    Science.gov (United States)

    Kim, Taeho; Kern, Elizabeth; Park, Chungoo; Nadler, Steven A; Bae, Yeon Jae; Park, Joong-Ki

    2018-05-10

    Mitochondrial genes and whole mitochondrial genome sequences are widely used as molecular markers in studying population genetics and resolving both deep and shallow nodes in phylogenetics. In animals the mitochondrial genome is generally composed of a single chromosome, but mystifying exceptions sometimes occur. We determined the complete mitochondrial genome of the millipede-parasitic nematode Ruizia karukerae and found its mitochondrial genome consists of two circular chromosomes, which is highly unusual in bilateral animals. Chromosome I is 7,659 bp and includes six protein-coding genes, two rRNA genes and nine tRNA genes. Chromosome II comprises 7,647 bp, with seven protein-coding genes and 16 tRNA genes. Interestingly, both chromosomes share a 1,010 bp sequence containing duplicate copies of cox2 and three tRNA genes (trnD, trnG and trnH), and the nucleotide sequences between the duplicated homologous gene copies are nearly identical, suggesting a possible recent genesis for this bipartite mitochondrial genome. Given that little is known about the formation, maintenance or evolution of abnormal mitochondrial genome structures, R. karukerae mtDNA may provide an important early glimpse into this process.

  4. [Taxonomy and evolution of the genus Pratylenchoides (Nematoda: Pratylenchidae)].

    Science.gov (United States)

    Ryss, A Iu

    2007-01-01

    The amended diagnosis of the genus Pratylenchoides and list of its valid species with synonyms are given. All the efficient diagnostic characters are listed. Modern taxonomic standard for the description of Pratylenchoides species is proposed; it may be used also in taxonomic databases. Tabular and text keys for all species of the genus are given. Five following groups are considered within the genus Pratylenchoides. The group arenicola differs from other groups in the primitive adanal bursa type; the groups magnicauda, crenicauda, ritteri, and megalobatus differ from each other in the position of cardium along the body axis in relation to the pharyngeal gland nuclei, pharynx types are named according to the stages of its evolution from the primitive tylenchoid pharynx (cardium situated posteriorly) to the advanced hoplolaimoid one (cardium situated anteriorly). Diagnoses and species compositions of the groups are given. Basing on the matrix of species characters, the dendrogram has been generated for all species of Pratylenchoides and for all characters (UPGMA, distance, mean character difference, random, characters ordered). Taking in view that the PAUP software gives equal weights to all characters, including the most important ones which define the prognostic species groups, the separate dendrograms for each prognostic species group were generated using the same above mentioned tree parameters. On the base of the records of Pratylenchoides species the matrices of plant host ranges, geographic distribution, and preferred soil-climatic conditions were developed. The dendrograms of the faunal similarities were generated using these matrices, with conclusions on a possible origin and evolution of the genus. The genus evolved from the flood lands with swampy soils and prevalence of dicotyledons (herbaceous Lamiaceae and woody Salicaceae families) to the forest mainland communities with balanced humidity and predominance of herbaceous Poaceae and Fabaceae with woody Fagaceae, Betulaceae, and Oleaceae. The leading factor of the evolutional adaptation to soil-climatic conditions was the factor of humidity, but its significance gradually decreased with the host change to more advanced plant taxa adapted to the communities with more dry balanced humidity. The genus took its origin on the south shores of Laurasia in the Cainozoe. Later, when Hindistant and Arabian Peninsula joined with Laurasia creating the Himalayas barrier, the Pratylenchoides spp. distributed by two branches: the northern one moved into Central Asia, East Europe and North America, and the south branch came into Indo-Malaya, West Asia and the north of Africa. The remnants of the ancient species groups remain in West Europe and East Asia. In the North America the genus gave an origin to its sister genus Apratylenchoides, which spread to the south up to Antarctica; another advanced branch spread in the North America reaching Alaska.

  5. Description of Globodera ellingtonae n. sp. (Nematoda: Heteroderidae) from Oregon.

    Science.gov (United States)

    Handoo, Zafar A; Carta, Lynn K; Skantar, Andrea M; Chitwood, David J

    2012-03-01

    A new species of cyst nematode, Globodera ellingtonae, is described from soil collected from a field in Oregon. Second-stage juveniles (J2) of the species are characterized by body length of 365-515 μm, stylet length of 19-22.5 μm, basal knobs rounded posteriorly and pointed anteriorly, tail 39-55 μm, hyaline tail terminus 20-32.5 μm, and tail tapering uniformly but abruptly narrowing and constricted near the posterior third of the hyaline portion, ending with a peg-like, finely rounded to pointed terminus. Cysts are spherical to sub-spherical, dark to light brown and circumfenestrate and cyst wall pattern is ridge-like with heavy punctations. Males have a stylet length of 21-25 μm and spicule length of 30-37 μm with a pointed thorn-like tip. Females have a stylet length of 20-22.5 μm, one head annule and labial disc, heavy punctations on the cuticle, and short vulval slit 7.5-8 μm long. Morphologically this new, round-cyst species differs from the related species G. pallida, G. rostochiensis, G. tabacum complex and G. mexicana by its distinctive J2 tail, and by one or another of the following: shorter mean stylet length in J2, females and males; number of refractive bodies in the hyaline tail terminus of J2; cyst morphology including Granek's ratio; number of cuticular ridges between the anus and vulva; and in the shape and length of spicules in males. Its relationship to these closely related species are discussed. Based upon analysis of ribosomal internal transcribed spacer (ITS) sequences, G. ellingtonae n. sp. is distinct from G. pallida, G. rostochiensis, G. tabacum and G. mexicana. Bayesian and Maximum Parsimony analysis of cloned ITS rRNA gene sequences indicated three clades, with intraspecific variability as high as 2.8%. In silico analysis revealed ITS restriction fragment length polymorphisms for enzymes Bsh 1236I, Hinf I, and Rsa I that overlap patterns for other Globodera species.

  6. Oncholaim~ jessicae n.sp. (Nematoda: Oncholaimidae) from fresh ...

    African Journals Online (AJOL)

    J. Heyns. Department of Zoology, Rand Afrikaans University, Johannesburg. Oncholaimus ... Body usually slightly ventrally curved to C-shaped, rarely almost straight upon fIxation. ..... Type locality. Various sites in the upper Blyde River system.

  7. Reassignment of Lamanema from Nematodirinae to Molineinae (Nematoda: Trichostronglyloidea).

    Science.gov (United States)

    Rickard, L G; Hoberg, E P

    2000-06-01

    The monospecific Lamanema historically has been assigned to the Nematodirinae within the Molineidae. Inconsistencies in morphological characters, within a phylogenetic context for Nematodirinae, led to a re-evaluation of the putative relationships and taxonomic placement of Lamanema. Among 7 putative synapomorphies for Nematodirinae, Lamanema possesses only 1, large eggs. Large eggs, sporadically present in phylogenetically disparate taxa of trichostrongyles, are equivocal with respect to placement of Lamanema; it is considered that possession of this single homoplasious character alone is insufficient justification to retain the genus in Nematodirinae. Affinities with the Trichostrongylidae (Cooperiinae or Haemonchinae) have also been proposed; however, Lamanema possess neither of 2 synapomorphies that diagnose monophyly of the family. Lamanema is retained in the Molineidae and transferred to the Molineinae as it possesses all characters of the family as currently defined. The origin of Lamanema represents a secondary colonization of ruminants by molineids and provides no context for elucidating the history of the Nematodirinae and Nematodirus.

  8. [Nematodirinae (Nematoda) from Ruminants and from lagomorpha. (author's transl)].

    Science.gov (United States)

    Durette-Desset, M C

    1979-01-01

    Study of eight species of Nematodirinae with special emphasis on their synlophe: Nematodirus filicollis (Rudolphi, 1802), N. spathiger (Railliet, 1896). N. helvetianus May, 1920, N. battus Crofton & Thomas, 1951, N. tortuosus Tucker, 1942, Nematodirella dromedarii (May, 1920), Nematodiroides zembrae (Bernard, 1965) and Rauschia triangularis, type species of the new genus Rauschia. Furthermore, bibliographical data permit to know the structure of the synlophe in four other species. In each of these species the synlophe retains the primitive bilateral symmetry observed in the Molineidae; in species parasitic in Ruminants and Rodents, the synlophe shows non pronounced size gradient, nor a pronounced peculiar orientation of the tip of the crests. These last specialized characters are observed, on the contrary, in species parasitic in Lagomorpha. These latter show, starting from synlophes of the "Anoplostrongylinae"-type, various evolutionary essays; the most remarkable is an hypertrophy of the dorsal crests which leads at the end of the evolution, to a dextral coiling, the back of the animal being inside the spire: such a position appears unique in the superfamily. Rauschia gen. nov. (type species: R. triangularis) is created for species previously pertaining to Nematodirus parasite of Lagomorpha, and in which the synlophe, very complex, differs from the synlophe of the parasite of Ruminants. A dichotomic key of the six genera of Nematodirinae is proposed.

  9. Two cystidicolids (Nematoda, Cystidicolidae) from marine fishes off New Caledonia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.

    2009-01-01

    Roč. 54, č. 4 (2009), s. 341-349 ISSN 1230-2821 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cystidicolid nematode * marine fish * New Caledonia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.070, year: 2009

  10. Book review: Systematics of Cyst Nematodes (Nematoda: Heteroderinae)

    Science.gov (United States)

    The cyst nematodes are an important group of plant-parasitic nematodes that cause billions of dollars in economic damage to crops every year. This article reviews a recently published, two-volume monograph that describes the morphological and molecular characteristics of these agriculturally signif...

  11. PENGARUH TEMPERATUR TERHADAP PERKEMBANGAN NEMATODA SISTA KENTANG (GLOBODERA SPP. INDONESIA

    Directory of Open Access Journals (Sweden)

    Lisnawita, Meity S. Sinaga Supramana & Gede Suastika .

    2011-11-01

    Full Text Available The effect of temperature on the development of Indonesian potato cyst nematode.  Potato cyst nematode (PCN is a serious pathogen of potato and is found in most potato producing areas of the world.  In Indonesia, PCN was recently discovered and recognized as major constraint on potato.  The growth and behaviour of nematodes are affected by the environment.  Therefore, determination of the biology of PCN Indonesian populations is urgently needed to manage this pathogens effectively.  The effect of temperature on biology factors of four PCN Indonesian populations (North Java 1, North Java 2, West Java, and Central Java was studied at different temperatures levels (12, 15, 18, 21 and 24ºC in the growth chamber.  The aim of this research was to study the effect of temperature on development of PCN.  Observation was conducted on cyst number, reproduction factor (Rf, survival, fecundity and multiplication of PCN.   The optimum temperature range for maximum cysts production with the highest reproduction factor, survival, fecundity and multiplication rate for all populations was 15 - 21ºC.  The hatching and development of all PCN populations reduced at temperature below 15ºC or above 21ºC.

  12. Redescription of three South African Monhystera species (Nematoda)*

    African Journals Online (AJOL)

    1993-07-22

    Jul 22, 1993 ... nuwere Qegewens omtrent die morfologie van die Monhysteridae . • Adapted from a D.Se. thesis submitted to the State University of Ghent. Belgium. by the senior author. •• To whom correspondence should be addressed at: Department of Zoology, Rand Afrikaans University, P.O. Box. 524, Auckland Park ...

  13. KARAKTERISTIK KOMUNITAS NEMATODA DI PADANG GOLF SUKARAME (PGS BANDAR LAMPUNG

    Directory of Open Access Journals (Sweden)

    I Gede Swibawa dan Titik Nur Aeny .

    2012-02-01

    Full Text Available ABSTRACT Characteristic of  Nematodes Community at PGS Golf Course Bandar Lampung. Golf course is a unique ecosystem where plant parasitic nematodes inhabit and become  important pest because they reduce the quality of the grass.  The pest problems on golf course can be indicated by its characteristics of nematode community.  Survey on nematodes community at PGS Golf Course Bandar Lampung was conducted from March to December 2004.  The objective was to study the characteristics of nematode community in the part of golf courses hole (green, collar, apron, and fairway.  Soil samples were taken from five holes each of it consists of green, collar, apron, and fairway, respectiveley.  Nematodes extraction was done in Laboratory of Arthropod Pest, Department of Plant Protection University of Lampung.   The result showed that at least 50 nematodes genera of 28 families inhabit PGS. The number of plant parasitic nematode genera was higher than the free-living one.  The characteristic of nematodes community on green and collar was different than on faiway. Nematode abundance and diversity on green and collar were higher than on fairway.  Based on abundance of nematode feeding group, maturity index of free-living nematodes (MI and that of plant parasitic nematodes (PPI, green and collar part seemed to be more favorable to plant parasitic nematodes than free-living nematodes. The contrary happened for fairway.  Four most dominant genera of plant parasitic nematodes on PGS golf course were Helicotylenchus, Hoplolaimus, Criconemella, and Xiphinema.

  14. (Nematoda: Desmodoridae), a brood protecting free-living marine ...

    African Journals Online (AJOL)

    posterior of the pharyngeal region, in the former species, in the male amphids which are a closed loop in P. waweri and an open-loop in P. ovisetosum. The female of the species protects the developing eggs by attaching them to the ventral mid body anterior and posterior of the vulval region, while in P. ovisetosum eggs are ...

  15. Redescription of Heterodera fici (Nematoda: Heteroderidae) with SEM Observations

    OpenAIRE

    Golden, A. Morgan; Maqbool, M. A.; Shahina, F.

    1988-01-01

    Heterodera fici is redescribed and illustrated with comparative details and revised measurements and diagnostic characters of the females, males, cysts, and juveniles from Maryland and Pakistan. This species is in the "schachtii group" (cysts lemon shaped, with bullae, and ambifenestrate) but the fenestrae in some cysts, presumab!y young ones, are small and widely spaced, appearing bifenestrate. It is most closely related to H. schachtii, H. glycines, and H. cajani but differs from these spec...

  16. (Nematoda, Desmodoridae) from Southern Africa and the North Sea

    African Journals Online (AJOL)

    1988-01-25

    Jan 25, 1988 ... Gub. length 110m. Sperm cells. 1Lffi0. Pre-anal suppl. Vaginal region. Epidermal cells. (j'. 9. C. granu- losus. 1105-1228. 1125-1196. 35-40. 30. 5. 14-17. 'consttic- tor cells' granular dense. C. longi- spiculosa. 2265-2470. 2-2510. 69-70. +. 25-30. 21-27 porids. C. na- mibiensis. 1734-1818. 1514-1788.

  17. Camallanus tridentatus (Drasche) (Nematoda: Camallanidae): new taxonomically important morphological data.

    Science.gov (United States)

    Santos, Cláudia Portes; Moravec, Frantisek

    2009-02-01

    Camallanus tridentatus is redescribed on the basis of the examination of specimens obtained from the stomach, caeca and intestine of the naturally infected arapaima Arapaima gigas (Schinz) from the Mexiana Island, Amazon River Delta, Brazil. Data on the surface morphology of adults inferred from confocal laser scanning and scanning electron microscopical observations are also provided. The study revealed some taxonomically important, previously unreported morphological features in this species, such as the presence of the poorly sclerotized left spicule and deirids. C. tridentatus distinctly differs from other congeneric species parasitizing freshwater fishes in South America mainly in the structure of the buccal capsule and the female caudal end. C. maculatus Martins, Garcia, Piazza and Ghiraldelli is considered a junior synonymm of Camallanus cotti Fujita.

  18. Camallanus tridentatus (Drasche (Nematoda: Camallanidae: new taxonomically important morphological data

    Directory of Open Access Journals (Sweden)

    Cláudia Portes Santos

    2009-02-01

    Full Text Available Camallanus tridentatus is redescribed on the basis of the examination of specimens obtained from the stomach, caeca and intestine of the naturally infected arapaima Arapaima gigas (Schinz from the Mexiana Island, Amazon River Delta, Brazil. Data on the surface morphology of adults inferred from confocal laser scanning and scanning electron microscopical observations are also provided. The study revealed some taxonomically important, previously unreported morphological features in this species, such as the presence of the poorly sclerotized left spicule and deirids. C. tridentatus distinctly differs from other congeneric species parasitizing freshwater fishes in South America mainly in the structure of the buccal capsule and the female caudal end. C. maculatus Martins, Garcia, Piazza and Ghiraldelli is considered a junior synonymm of Camallanus cotti Fujita.

  19. Camallanus tridentatus (Drasche) (Nematoda: Camallanidae): new taxonomically important morphological data

    Czech Academy of Sciences Publication Activity Database

    Santos, C. P.; Moravec, František

    2009-01-01

    Roč. 104, č. 1 (2009), s. 93-99 ISSN 1678-8060 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Camallanus * Arapaima * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine

  20. Ultrastructure of the adults of Bunostomum phlebotomum (Nematoda: Ancylostomatidae

    Directory of Open Access Journals (Sweden)

    Antônio Cesar Rios Leite

    1992-01-01

    Full Text Available The morphology of the male and female of Bunostomum phlebotomum are described based on scanning electron microscope (SEM observations. The attachment of the worms to the small intestinal mucosa and during the copula were observed. Structures of the bucal capsule and genital organs were also studied.

  1. Nematoda from the terrestrial deep subsurface of South Africa

    NARCIS (Netherlands)

    Borgonie, G.; García-Moyano, A.; Litthauer, D.; Bert, W.; Bester, A.; Heerden, van E.; Möller, C.; Erasmus, M.; Onstott, T.C.

    2011-01-01

    Since its discovery over two decades ago, the deep subsurface biosphere has been considered to be the realm of single-cell organisms, extending over three kilometres into the Earth’s crust and comprising a significant fraction of the global biosphere1–4. The constraints of temperature, energy,

  2. Diversity of Mammomonogamus (Nematoda: Syngamidae) in large African herbivores

    Czech Academy of Sciences Publication Activity Database

    Červená, B.; Hrazdilová, K.; Vallo, Peter; Pafčo, B.; Fenyková, T.; Petrželková, Klára Judita; Todd, A.; Tagg, N.; Wangue, N.; Lux Hoppe, E. G.; Duarte Moraes, M. F.; Lapera, I. M.; Souza Pollo, A.; Albuquerque, A. C. A.; Modrý, D.

    2018-01-01

    Roč. 117, č. 4 (2018), s. 1013-1024 ISSN 0932-0113 R&D Projects: GA ČR GA15-05180S Institutional support: RVO:68081766 Keywords : gorilla-gorilla-gorilla * du-petit-loango * mitochondrial DNA * genetic diversity * host-specificity * forest * populations * sequence * endoparasites * strongylida * Mammomonogamus * Gorilla * African forest elephant * African forest buffalo * Parasite sharing * Host specificity Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 2.329, year: 2016

  3. Two Procamallanus spp. (Nematoda, Camallanidae) from fishes in the Philippines

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Cruz-Lacierda, E. R.; Nagasawa, K.

    2004-01-01

    Roč. 49, č. 4 (2004), s. 309-318 ISSN 1230-2821 R&D Projects: GA AV ČR IAA6022201 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematode * Procamallanus * Spirocamallanus Subject RIV: EA - Cell Biology Impact factor: 0.560, year: 2004

  4. Calodium (Capillaria hepaticum (Nematoda, Capillariidae in insular small rodent populations

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    Bugmyrin Sergey

    2014-12-01

    Full Text Available The data on the distribution of the nematode Calodium hepaticum (Bancroft 1893 Moravec 1982 (syn.: Capillaria hepatica, Hepaticola hepatica on the islands of Kizhi Archipelago are reported (N 62°00'; E 35°12'. Samples were collected on 18 islands and the mainland part of the Kizhi skerries region in the period from August 2005 till 2014. The method of partial helminthological dissection was applied to 346 specimens of rodents belonging to two species – the bank vole Myodes glareolus Schreber 1780 (301 spm. and the field vole Microtus agrestis Linnaeus 1761 (45 spm.. The prevalence and the abundance index of nematode were 16.6% and 1.1 in M. glareolus and 11.1%; 0.3 in M. agrestis, respectively. The highest prevalence and abundance of C. hepaticum were detected in mature voles. No sex-related differences were found. C. hepaticum was present in 12 of 19 sampling sites. On the islands where the sample number (host individuals was over 15, the highest prevalence and abundance values were 57% and 5.8 spm., respectively. Significant positive coefficients of correlation (Spearman’s and Pearson’s ones between nematode numbers and characteristics of the island were found in the pair «Prevalence – degree of isolation» (0.48 and 0.49. Single-factor analysis of variance showed that the size of the island had some effect on the nematode invasion prevalence and abundance. However, no significant regression relationship between the prevalence and abundance of nematodes and characteristics of an island was revealed by multivariate regression analysis (multiple regression: the coefficient of determination of the regression equation R2 < 0.3, and the regression coefficients were insignificant The reasons for high abundance of C. hepaticum in northern insular ecosystems are discussed. Possible key factors for the stable vitality of the parasite populations are: 1 favourable hydrothermal conditions of the soil in the shore (littoral zone; 2 the possibility of passive transfer between islands; 3 the ability to survive outside the host for a long time (an egg can remain invasive in the external environment for up to three years; and 4 cannibalism as the main pathway of vermination circulation in isolated populations of small mammals.

  5. Kontaminasi Telur Cacing pada Sayur dan Upaya Pencegahannya

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    Hebert Adrianto

    2017-12-01

    Full Text Available ABSTRAK Infeksi cacing merupakan permasalahan yang banyak ditemukan di negara berkembang dan belum tuntas diselesaikan. Sayur lalapan dapat menjadi media penularan telur cacing ke manusia. Tujuan tulisan ini adalah mengidentifikasi jenis telur cacing yang ditemukan pada sayuran dan upaya pencegahan menurut siklus hidup cacing tersebut. Tulisan ini mengkaji 4 jurnal penelitian Indonesia, 22 jurnal penelitian internasional, website WHO, dan buku teks yang relevan dengan topik kajian. Hasil kajian menunjukkan jenis telur cacing yang ditemukan pada sayuran tidak hanya Nematoda Soil Transmitted Helminth (STH seperti Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale, dan Strongyloides stercoralis, tetapi juga Nematoda lainnya (Trichostrongylus, Toxocara, Trichocephal, dan Enterobius vermicularis, kelompok cacing Cestoda (Taenia spp., Hymenolepis nana, dan H. diminuta, kelompok Trematoda (Heterophyes heterophyes, Clonorchis sinensis, Fasciola, dan Dicrocoelium dendriticum. Setiap spesies cacing memiliki siklus hidup dan hospes perantara yang berbeda. Upaya pencegahan untuk menghindari risiko infeksi memerlukan kerja sama lintas program, lintas sektor, dan masyarakat dengan memperhatikan siklus hidup cacing. Kata kunci: sayur, telur cacing, pencegahan, kerjasama ABSTRACTHelminth infections are the most common infections in developing countries and still need to be eradicated. Fresh vegetables can be the source for transmission of helminth egg to humans. The aims of this paper are to identify the types of helminth egg which found in vegetables and prevention efforts according to the helminth life cycle. A literature review was arranged by review of 4 articles Indonesian research journal, 22 articles non Indonesia research journal, WHO website, and textbooks relevant to the study topic. The paper describes list of helminth eggs in vegetables, environmental, behaviors, and host-related helminth analyzes in vegetables

  6. The response of Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae) and Steinernema feltiae (Nematoda: Steinernematidae) to different host-associated cues

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk

    2012-01-01

    Roč. 61, č. 3 (2012), s. 201-206 ISSN 1049-9644 R&D Projects: GA MŠk(CZ) ME10078 Institutional research plan: CEZ:AV0Z50070508 Keywords : chemoattraciton * entomopathogenic nematode * gastropod parasitic nematode Subject RIV: EH - Ecology, Behaviour Impact factor: 1.917, year: 2012 http://www.sciencedirect.com/science/article/pii/S1049964412000436

  7. Systematics of gastrointestinal nematodes of domestic ruminants: advances between 1992 and 1995 and proposals for future research.

    Science.gov (United States)

    Lichtenfels, J R; Hoberg, E P; Zarlenga, D S

    1997-11-01

    individual species as components of mixed populations and requires highly trained specialists. Available identification keys for larvae are not well illustrated and need to be augmented. Adults: Recent advances in the identification of adult trichostrongyloids and their systematics are organized by taxonomic group. Genera included are Ostertagia, Haemonchus, Cooperia, Trichostrongylus and Nematodirus. Recently, the first phylogenetic analysis of the Trichostrongylidae family established monophyly for the family. A similar analysis of the Molineidae is needed. Ostertagia: Several studies of polymorphism summarized the phenomenon and listed 19 polymorphic species in five genera. Two studies of DNA differences within and among polymorphic species of Ostertagiinae supported earlier hypotheses that the species pairs represent polymorphic species. A phylogenetic analysis of the Ostertagiinae and generic concepts are needed. Haemonchus: A key to three species of Haemonchus provides, for the first time, morphological characteristics for the microscopical identification to species of individual adult nematodes of either sex. The Food and Drug Administration is now requiring that results of drug trials include identification of Haemonchus to species. Cooperia: Studies using random amplified polymorphic DNA methods showed a high degree of variation within and among C. oncophora/C. surnabada, but supported a polymorphic relationship for the species pair. A phylogenetic analysis of the Cooperiinae is needed. Trichostrongylus: Restriction Fragment Length Polymorphisms (RFLPs) of genomic DNA of two strains of T. colubriformis indicated a high degree of intra- and inter-strain DNA polymorphism. However, other studies demonstrated expected species level differences between T. colubriformis and T. vitrinus using Random Amplified Polymorphic DNA (RAPD) methods. Sequences of the second Internal Transcribed Spacer Region (ITS-2) ribosomal repeat showed sequence differences of 1.3-7.6% among five

  8. Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae).

    Science.gov (United States)

    Mohan, Sharad; Mauchline, Tim H; Rowe, Janet; Hirsch, Penny R; Davies, Keith G

    2012-03-01

    The Pasteuria group of Gram-positive, endospore-forming bacteria are parasites of invertebrates and exhibit differences in host specificity. We describe a cross-infection study between an isolate of Pasteuria from pigeon pea cyst nematode, Heterodera cajani, which also infects the potato cyst nematode, Globodera pallida, from the United Kingdom. A proportion of the attached endospores, 13% on H. cajani and 22% on G. pallida adhere to the cuticle in an inverted orientation. Inverted and conventionally attached endospores germinated and produced bacillus-like rods that completed their life cycle in Pasteuria population was systematically followed in two different nematode genera. A 1430-base pair fragment of the 16S rRNA gene sequence of the Pasteuria isolate from H. cajani revealed 98.6% similarity to the orthologous gene in Pasteuria nishizawae. Additionally, their respective endospore sizes were not significantly different, in contrast their host ranges are. Potential reasons for this remain unclear and are discussed. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  9. Helminths in Mesaspis monticola (Squamata: Anguidae from Costa Rica, with the description of a new species of Entomelas (Nematoda: Rhabdiasidae and a new species of Skrjabinodon (Nematoda: Pharyngodonidae

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    Bursey C.R.

    2006-09-01

    Full Text Available Entomelas duellmani n. sp. (Rhabditida: Rhabdiasidae from the lungs and Skrjabinodon cartagoensis n. sp. (Oxyurida: Pharyngodonidae from the intestines of Mesaspis monticola (Sauria: Anguidae are described and illustrated. E. duellmani is the sixth species assigned to the genus and is the third species described from the Western Hemisphere. It is easily separated from other neotropical species in the genus by pre-equatorial position of its vulva. Skrjabinodon cartagoensis is the 24th species assigned to the genus and differs from other neotropical species in the genus by female tail morphology.

  10. New species of Oswaldocruzia (Nematoda: Molineoidae), new species of Rhabdias (Nematoda: Rhabdiasidae), and other helminths in Rana cf. forreri (Anura: Ranidae) from Costa Rica.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R

    2005-06-01

    Oswaldocruzia costaricensis n. sp. (Strongylida: Molineidae) from the intestines and Rhabdias savagei n. sp. (Rhabditida: Rhabdiasidae) from the lungs of Rana cf. forreri (Anura: Ranidae) are described and illustrated. Oswaldocruzia costaricensis represents the 77th species assigned to the genus and differs from the other Neotropical species in the genus by possessing a Type II bursa and long cervical alae. Rhabdias savagei represents the 47th species assigned to the genus and differs from other Neotropical species in the genus by possession of 4 lips and a postequatorial vulva. Rana cf. forreri was also found to harbor the trematodes, Haematoloechus parcivitellarius and Megalodiscus temperatus, the nematodes, Aplectana incerta, Aplectana itzocanensis, Cosmocerca podicipinus, Foleyellides striatus, Subulascaris falcaustriformis, and a larva of the nematode Brevimulticaecum sp. Cosmocerca panamaensis is considered to be a synonym of Cosmocerca podicipinus.

  11. Contracaecumovale (Nematoda: Anisakidae from Rollandia rolland Quoy & Gaimard 1824 (Aves, Podicipedidae in Argentina Contracaecumovale (Nematoda: Anisakidae de Rollandia rolland Quoy & Gaimard 1824 (Aves, Podicipedidae na Argentina

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    Noelia Adelina Galeano

    2012-06-01

    Full Text Available Necropsy on 15 specimens of white-tufted grebe, Rollandiarolland, caught in the Mar Chiquita and Chascomús lagoons (Buenos Aires province, revealed the presence of Contracaecumovale (Linstow, 1907. This nematode shows a marked specificity for podicipediform birds. The specimens were identified from morphological study on features such as cephalic and esophageal structures and caudal papillae, using both optical and scanning electron microscopy. This is the first record of C. ovale parasitizing R. rolland in Argentina.Necropsia de 15 espécimes de mergulhão-de-orelha-branca, Rollandiarolland, coletados nas lagoas Mar Chiquita e Chascomús (Província de Buenos Aires, revelou a presença de Contracaecumovale (Linstow, 1907. Esse nematóide tem uma marcada especificidade pelas aves podicipediformes. Os espécimes foram identificados a partir de características, tais como estruturas morfológicas cefálicas e esofágicas e papilas caudais, utilizando-se microscopia óptica e microscopia eletrônica de varredura (MEV. Esse é o primeiro registro de C. ovale parasito de R. rolland na Argentina.

  12. Método estimativo para amostragem quantitativa de Rondonia rondoni (Nematoda: Atractidae parasito de peixes Estimative method for quantitative sampling of Rondonia rondoni (Nematoda: Atractidae parasite of fish

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    Cristiane M. De Campos

    2007-09-01

    Full Text Available O objetivo deste trabalho foi avaliar o número de Rondonia rondoni no intestino de Piaractus mesopotamicus, por meio da diferença entre peso úmido e peso seco das amostras de parasitos para cada hospedeiro, a partir da relação do peso seco e número de parasitos pré-estabelecida. Amostras totais de R. rondoni, de 37 espécimes de Piaractus mesopotamicus, foram medidas para obtenção do peso úmido, desidratadas em estufa com temperatura entre 55ºC e 60ºC e, após 24 h seu peso seco foi determinado. Por meio de uma regra de três simples, calculou-se o número de parasitos a partir da diferença entre o peso úmido e o peso seco, considerando um erro padrão médio de 6,027 para um número médio de 1010 indivíduos, quantificado em ensaio prévio. A equação da regressão linear estimada foi de y = 13,138x - 162,01 e r² = 0,9989, a qual foi significativa (p The presente work evaluated the number of Rondonia rondoni in intestine of Piaractus mesopotamicus, for diference of dry and wet weight of parasites samples by each host, from the relation dry weight and number of parasites. Totals samples of R. rondoni of 37 specimens of P. mesopotamicus for attaiment of wet weight, dehydrated in stove with temperature between 55ºC and 60ºC, the wet weight was measured after 24 h. The number of parasites was calculated with rule of three, considering the average number of 1,010 ± 6.027 specimens quantified in previous assay. The equation of linear regression is y = 13.138x - 162.01 and r² = 0.9989. The normality of the data was proven with Kolmogorov-Smirnov (p < 0.01.

  13. Angiostrongylus vasorum (Baillet, 1866 Nematoda: Prostostrongylidae, em cães de Minas Gerais, Brasil Angiostrongylus vasorum (Baillet, 1866 Nematoda: Prostostrongylidae, in dogs of Minas Gerais, Brasil

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    Walter dos Santos Lima

    1985-06-01

    Full Text Available Foi identificado Angiostrongylus vasorum (Baillet, 1866 colhido da artéria pulmonar de dois cães (Canis familiaris procedentes do município de Caratinga, Estado de Minas Gerais, Brasil. É apresentada a descrição morfológicas do parasita. Esta é a primeira referência desse parasita no Estado de Minas Gerais.For the first time Angiostrongylus vasorum in Canis familiaris in Minas Gerais State, Brazil, is described. The description and measurements of three males and ten females are presented together with a diagram of the parasite.

  14. Systematics of the genus Gnathostoma (Nematoda: Gnathostomatidae in the Americas Sistemática del género Gnathostoma (Nematoda: Gnathostomatidae en América

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    Florencia Bertoni-Ruiz

    2011-06-01

    Full Text Available To date, more than 20 species of the genus Gnathostoma have been described as parasites of mammals, 9 of them in the Americas. However, the taxonomic status of some of these species has been questioned. The main goal of this study is to clarify the validity of the American species included in the genus. In order to complete this objective, we analyze type and/or voucher specimens of all these species deposited in 6 scientific collections, through morphometric and ultrastructural studies. Based on diagnostic traits as host specificity, site of infection, body size, cuticular spines, presence of 1 or 2 bulges in the polar ends of eggs, as well as eggshell and caudal bursa morphology, we re-establish Gnathostoma socialis (Leidy, 1858 and confirm the validity of other 6 species: Gnathostoma turgidum Stossich, 1902, Gnathostoma americanum Travassos, 1925, Gnathostoma procyonis Chandler, 1942, Gnathostoma miyazakii Anderson, 1964, Gnathostoma binucleatum Almeyda-Artigas, 1991, and Gnathostoma lamothei Bertoni-Ruiz, García-Prieto, Osorio-Sarabia and León-Règagnon, 2005. Gnathostoma didelphis Chandler, 1932 and Gnathostoma brasiliensis Ruiz, 1952 are considered synonyms of G. turgidum. Finally, based on a wide revision of specimens deposited in 6 American collections, we conclude that records of Gnathostoma spinigerum Owen, 1836 in the Americas are invalid.A la fecha, se han descrito más de 20 especies del género Gnathostoma parásitas de mamíferos, 9 de ellas en America. Sin embargo, el estado taxonómico de algunas ha sido cuestionado. El objetivo de este estudio es aclarar la validez de las especies americanas incluidas en el género. Para ello, se analizaron ejemplares tipo o de referencia de todas las especies, depositados en 6 colecciones científicas, mediante estudios morfométricos y ultraestructurales. Con base en rasgos diagnósticos como especificidad hospedatoria, sitio de infección, dimensiones corporales, espinas cuticulares (número de puntas, densidad, forma y distribución, presencia de uno ó dos tapones polares en los huevos, así como morfología de la bursa, patrón papilar y de la cubierta de los huevos, se re-establece a Gnathostoma socialis (Leidy, 1858 y se confirma la validez de otras 6 especies: Gnathostoma turgidum Stossich, 1902, Gnathostoma americanum Travassos, 1925, Gnathostoma procyonis Chandler, 1942, Gnathostoma miyazakii Anderson, 1964, Gnathostoma binucleatum Almeyda-Artigas, 1991 y Gnathostoma lamothei Bertoni-Ruiz, García-Prieto, Osorio Sarabia y León-Règagnon, 2005. Gnathostoma didelphis Chandler, 1932 y Gnathostoma brasiliensis Ruiz, 1952 son consideradas sinónimos de G. turgidum. Basados en una amplia revisión de material depositado en 6 colecciones Americanas, se invalidan los registros de Gnathostoma spinigerum Owen, 1836 en America.

  15. Tissue alterations in the pirarucu, Arapaima gigas, infected by Goezia spinulosa (Nematoda Alterações teciduais em pirarucu, Arapaima gigas, infectado por Goezia spinulosa (Nematoda

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    Rodrigo Caldas Menezes

    2011-09-01

    Full Text Available Five specimens of Arapaima gigas caught in the Araguaia River (State of Mato Grosso, Brazil were investigated for helminths in 2004. Numerous adult specimens of the rhapidascarid nematode Goezia spinulosa were found in stomach ulcers in all the specimens of A. gigas and were surrounded by thickening of the mucosa. The gastric glands of all the fish were necrotic and there was a severe and diffuse inflammatory reaction composed of eosinophils (which were predominant, lymphocytes and rare macrophages in the mucosa, submucosa and muscle layer. This is the first report of tissue lesion occurrences in this host, in the presence of G. spinulosa, and it confirms the high pathogenicity of this parasite species.Cinco espécimens de Arapaima gigas capturados no Rio Araguaia (Estado do Mato Grosso, Brasil foram investigados para diagnóstico de infecção por helmintos em 2004. Numerosos espécimes adultos do nematóide rafidascarídeo Goezia spinulosa foram encontrados em úlceras do estômago circundadas por um espessamento da mucosa em todos os exemplares de A. gigas. As glândulas gástricas de todos encontravam-se necróticas e havia um acentuado e difuso infiltrado inflamatório composto por eosinófilos, que eram predominantes, linfócitos e raros macrófagos na mucosa, submucosa e camada muscular. As lesões teciduais na presença de nematóide G. spinulosa são relatadas pela primeira vez nesse hospedeiro e confirmam a alta patogenicidade dessa esp��cie de parasito.

  16. Population structure of Angiostrongylus cantonensis (Nematoda: Metastrongylidae) in Thailand based on PCR-RAPD markers.

    Science.gov (United States)

    Thaenkham, Urusa; Pakdee, Wallop; Nuamtanong, Supaporn; Maipanich, Wanna; Pubampen, Somchit; Sa-Nguankiat, Surapol; Komalamisra, Chalit

    2012-05-01

    Angiostrongylus cantonensis is the causative agent of angiostrongyliasis, which is widely distributed throughout the world. It can specifically infect many species of intermediate and definitive hosts. This study examined the genetic differentiation and population structure using the RAPD-PCR method of parasites obtained from 8 different geographical areas of Thailand. Based on 8 primers, high levels of genetic diversity and low levels of gene flow among populations were found. Using genetic distance and neighbor-joining dendrogram methods, A. cantonensis in Thailand could be divided into two groups with statistically significant genetic differentiation of the two populations. However, genotypic variations and haplotype relationships need to be further elucidated using other markers.

  17. Rhabditid species (Nematoda, Rhabditida recorded in peninsular Spain and Balearic Islands

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    Abolafia, Joaquín

    2001-06-01

    Full Text Available Previous records of species belonging to the order Rhabditida in peninsular Spain and Balearic Islands is presented as compilation. Eighty species of forty genera and twelve families are listed. Information concerning each species contains scientific name, synonymy, distribution and remarks. Distributional data include localities and/or geographical areas where the species have been collected and the corresponding references. A short discussion on current knowledge on rhabditid nematodes in the Iberian geography is also made. Majority of species are defficiently known, being necessary additional taxonomic and/or faunistic studies of this taxon.

    Se presenta una recopilación de citas previas de especies pertenecientes al orden Rhabditida en la España peninsular e Islas Baleares. Se listan ochenta especies de cuarenta géneros y doce familias. La información que concierne a cada especie contiene el nombre científico, sinonimia, distribución y observaciones. Los datos de distribución incluyen las localidades y/o áreas geográficas donde las especies han sido recolectadas, y las referencias correspondientes. También se realiza una breve discusión sobre el conocimiento de los nematodos rhabdítidos en el área ibérica, concluyéndose que la mayor parte de las especies se conocen deficientemente, siendo necesario un estudio taxonómico y/o faunístico adicional del taxon.

  18. New species of Thelonema, Metasphaerolaimus, and Monhystrella (Nematoda, Monhysterida from Kermadec Trench, Southwest Pacific

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    Daniel Leduc

    2015-12-01

    Full Text Available Three new species of the order Monhysterida are described based on specimens obtained at depths of 8081 and 9177 m in the Kermadec Trench. Thelonema clarki sp. nov. is characterised by a large body size (3230–4461 µm, short cylindrical buccal cavity, gubernaculum without apophyses, and long conico-cylindrical tail. This is the first record of the genus since its original description over two decades ago from the Peru Basin. Metasphaerolaimus constrictus sp. nov. is characterised by a relatively long body (1232–1623 µm, slightly arcuate spicules without gubernaculum, and conico-cylindrical tail with inner cuticle conspicuously thickened immediately anterior to cylindrical portion. Monhystrella kermadecensis sp. nov. is characterised by a circle of papillose outer labial sensillae slightly anterior to the four short cephalic setae, gubernaculum with caudal apophyses, the presence of distinct cuticularised piece along anterior vaginal wall, and a relatively short conical (males or conico-cylindrical tail (females with conical, ventrally-curved spinneret. M. kermadecensis sp. nov. can be differentiated from all other species of the genus, and, indeed, the entire family, based on the variable position of the anterior gonad relative to the intestine. The new species is classified within the Monhysteridae, and not the closely-related Xyalidae, based on the small body size, a smooth cuticle, and the presence of six outer labial papillae and only one testis. Further work is required to clarify the placement of M. kermadecensis sp. nov. relative to other monhysterid genera. A tabular key to all ten valid Metasphaerolaimus species is presented.

  19. Infective larvae of Rhabdiasidae (Nematoda): comparative morphology of seven European species.

    Science.gov (United States)

    Kuzmin, Yuriy; Junker, Kerstin; Bain, Odile

    2014-03-01

    The morphology of infective third-stage larvae of Rhabdias bufonis, R. rubrovenosa, R. sphaerocephala, R. fuscovenosa, R. elaphe, Entomelas entomelas and E. dujardini is described. The sheath structure in the studied larvae appeared to be similar to that described in other species of the family Rhabdiasidae, its chequered aspect being caused by a combination of outer longitudinal striations and inner longitudinal as well as transverse ridges. The larvae were similar in general morphology but differed in the presence/absence of anterior apical protuberances (pseudolabia), the shape and ornamentation of the tail tip, and the structure of lateral alae in the caudal region of the body. No relationship between the morphological characters of the larvae of the studied species and their taxonomic position or specificity of adult parasites to a particular host group was observed. Regardless, the larvae of each species can be identified by a combination of morphological peculiarities in the anterior and caudal regions of the body.

  20. Two new species of Rhabdochona (Nematoda: Rhabdochonidae) from freshwater fishes in Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Yooyen, T.

    2011-01-01

    Roč. 58, č. 3 (2011), 224–232 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : parasitic nematode * Rhabdochona * freshwater fish * Mystacoleucus * Pseudomystus * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.812, year: 2011 http://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=21989

  1. Studies on ascaridid, oxyurid and enoplid nematodes (Nematoda) from fishes of the Okavango River, Botswana

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Van As, L. L.

    2015-01-01

    Roč. 62, JUL 22 2015 (2015), s. 039 ISSN 1803-6465 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : helminth parasites, * taxonomy * new species, * Cucullanus * Cithariniella * Synodontisia * Galeiceps * freshwater fish * Africa Subject RIV: EG - Zoology Impact factor: 1.271, year: 2015

  2. The occurance of Pterygodermatites nycticebi (Nematoda: Rictulariidae) in a captive slow loris, Nycticebus coucang

    Science.gov (United States)

    Tuggle, B.N.; Beehler, B.A.

    1984-01-01

    Adult and immature rictulariid nematodes were recovered at necropsy from the small intestine of an adult slow loris, Nycticebus coucang, from the Milwaukee County Zoo in Wisconsin. The lumen of the entire small intestine was packed with more than 100 nematodes, the intestinal wall appeared thickened and the mucosal surface contained numerous petechial hemorrhagic foci. The cause of death was diagnosed as a septicemia and possible lupus erythematosis.

  3. Contributions to the genus Opisthodorylaimus Ahmad & Jairajpuri, 1982 (Nematoda: Dorylaimida, with descriptions of two new species

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    Andrássy, I.

    2007-08-01

    Full Text Available Two new species of the genus Opisthodorylaimus are described. O. mitis sp. n. from Guadeloupe and Ecuador is characterized by a transverse vulva, very long egg, lack of an anterior uterine sac, and by a filiform tail. O. papuanus sp. n. from Papua New Guinea is distinguished by a longitudinal vulva, lack of a prevulval uterine branch, and by a long, filiform tail. Two known species of the genus are also presented: O. cavalcantii (Lordello, 1955 from Vietnam and O. maqsoodi Ahmad & Jairajpuri, 1982 from Seychelles. The taxonomic position of Opisthodorylaimus is commented, and the distribution of its ten species outlined. A key to species is added.

  4. Steinernema beitlechemi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa

    Czech Academy of Sciences Publication Activity Database

    Çimen, H.; Půža, Vladimír; Nermuť, Jiří; Hatting, J.; Ramakuwela, T.; Faktorová, Lucie; Hazir, S.

    2016-01-01

    Roč. 18, č. 4 (2016), s. 439-453 ISSN 1388-5545 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : description * molecular * morphology Subject RIV: EG - Zoology Impact factor: 1.162, year: 2016

  5. Steinernema biddulphi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa

    Czech Academy of Sciences Publication Activity Database

    Çimen, H.; Půža, Vladimír; Nermuť, Jiří; Hatting, J.; Ramakuwela, T.; Hazir, S.

    2016-01-01

    Roč. 48, č. 3 (2016), s. 148-158 ISSN 0022-300X R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : D2-D3 * entomopathogenic nematodes * ITS Subject RIV: EG - Zoology Impact factor: 1.087, year: 2016 http://journals.fcla.edu/jon/issue/view/4275

  6. Orientatractis brycini sp. nov. (Nematoda: Atractidae) from characiform freshwater fishes in Gabon, Africa

    Czech Academy of Sciences Publication Activity Database

    González-Solís, David; Mariaux, J.

    2017-01-01

    Roč. 124, č. 1 (2017), s. 1-8 ISSN 0035-418X R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : new species * nematode * Brycinus * Xenocharax Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine OBOR OECD: Veterinary science Impact factor: 0.380, year: 2016

  7. Pharmacological receptors of nematoda as target points for action of antiparasitic drugs

    Directory of Open Access Journals (Sweden)

    Trailović Saša M.

    2010-01-01

    Full Text Available Cholinergic receptors of parasitic nematodes are one of the most important possible sites of action of antiparasitic drugs. This paper presents some of our own results of electrophysiological and pharamcological examinations of nicotinic and muscarinic receptors of nematodes, as well as data from literature on a new class of anthelmintics that act precisely on cholinergic receptors. The nicotinic acetylcholine receptor (nAChR is located on somatic muscle cells of nematodes and it is responsible for the coordination of parasite movement. Cholinomimetic anthelmintics act on this receptor, as well as acetylcholine, an endogenic neurotransmitter, but they are not sensitive to enzyme acetylcholineesterase which dissolves acetylcholine. As opposed to the nicotinic receptor of vertebra, whose structure has been examined thoroughly, the stoichiometry of the nicotinic receptor of nematodes is not completely known. However, on the grounds of knowledge acquired so far, a model has been constructed recently of the potential composition of a type of nematodes nicotinic receptor, as the site of action of anthelmintics. Based on earlier investigations, it is supposed that a conventional muscarinic receptor exists in nematodes as well, so that it can also be a new pharamocological target for the development of antinematode drugs. The latest class of synthesized anthelmintics, named aminoacetonitriles (AAD, act via the nicotinic receptor. Monepantel is the first drug from the AAD group as a most significant candidate for registration in veterinary medicine. Even though several groups of cholinomimetic anthelmintics (imiodazothiazoles, tetrahydropyrimidines, organophosphat anthelmintics have been in use in veterinary practice for many years now, it is evident that cholinergic receptors of nematodes still present an attractive place in the examinations and development of new antinematode drugs. .

  8. Spontaneous cure after natural infection with Gnathostoma turgidum (Nematoda) in Virginia opossums (Didelphis virginiana).

    Science.gov (United States)

    Torres-Montoya, Hilario; Galaviz-Renteria, Xochilth; Castillo-Ureta, Hipólito; Lopez-Moreno, Hector; Nawa, Yukifumi; Bojorquez-Contreras, Angel; Sanchez-Gonzalez, Sergio; Díaz-Camacho, Sylvia; Rocha-Tirado, Rodrigo; Rendon-Maldonado, Jose

    2014-04-01

    Seasonality of the nematode Gnathostoma turgidum in Virginia opossums (Didelphis virginiana) in the wild has been reported; however, the mechanisms involved in deworming are unknown. We monitored the parasitologic and biologic changes in four Virginia opossums naturally infected with G. turgidum by coproparasitologic examination and abdominal ultrasonography. Eggs became detectable in the feces of opossums in May, peaked in July and August, and suddenly decreased in October. Adults of G. turgidum were expelled in the feces mainly in September. Ultrasonography of the liver showed slight damage during May. Lesions in the stomach appeared in April and persisted until September. The abnormalities of the liver and stomach were resolved in November. These data suggest that G. turgidum is likely expelled as a result of host immunologic mechanisms, although termination of a natural life span cannot be definitively excluded.

  9. Strongylus asini (Nematoda, Strongyloidea): genetic relationships with other Strongylus species determined by ribosomal DNA.

    Science.gov (United States)

    Hung, G C; Jacobs, D E; Krecek, R C; Gasser, R B; Chilton, N B

    1996-12-01

    Genomic DNA was isolated from adult Strongylus asini collected from zebra. The second ribosomal transcribed spacer (ITS-2) was amplified and sequenced using polymerase chain reaction (PCR) based techniques. The DNA sequence was compared with previously published data for 3 related Strongylus species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. The ITS-2 sequence of S. asini was found to be more similar to those of S. edentatus (87.1%) and S. equinus (95.3%) than to that of S vulgaris (73.9%). This result confirms that S. Asini and S vulgaris represent separate species and supports the retention of the 4 species within 1 genus.

  10. Occurrence of Strongylidae (Nematoda: Strongyloidea) in Polish horses "tarpans" from Popielne Reserve.

    Science.gov (United States)

    Gawor, J

    2000-01-01

    The study was performed to evaluate intensity of strongylid infection in stabled tarpans using the method of collecting worms after anthelmintic treatment and in wild tarpans from Popielne reserve on the basis of faecal examination. After treatment with ivermectin 12 cyathostomes and one large strongyle species were recovered from the faeces of two tarpans. The most abundant cyathostome species were C. catinatum, C. pateratum, C. longibursatum, C. nassatum and C. coronatus. A higher intensity of infections with small strongyles (cyathostomes) was found in stabled group than in the group from the reserve. Large strongyles were more prevalent in wild tarpans, with Strongylus vulgaris as most common species (66.7%), but the intensity of infection was low. The present results are compared with earlier studies of horses in Poland and other countries. The results confirm the stability of cyathostomes in different breed of horses over the world.

  11. A new species of Procamallanus (Nematoda: Camallanidae) from Pacific eels (Anguilla spp.)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.; Würtz, J.; Taraschewski, H.; Sasal, P.

    2006-01-01

    Roč. 92, č. 1 (2006), s. 130-137 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Procamallanus * Anguilla Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.300, year: 2006

  12. Larval spirurida (Nematoda) from the crab Macrophthalmus hirtipes in New Zealand

    DEFF Research Database (Denmark)

    Moravec, Frantisek; Fredensborg, Brian Lund; Latham, A David M

    2003-01-01

    to a species of Ascarophis van Beneden, 1871 (Cystidicolidae), the genus including parasites of fishes, whereas the smaller larvae (about 4-5 mm long) belonged to the Acuariidae, a family with species parasitic as adults mostly in aquatic birds. In a sample of 82 specimens of M. hirtipes collected in July 2002....... Apparently, crabs play a role as intermediate hosts of these nematode species. This is the first record of larval representatives of Cystidicolidae and Acuariidae from invertebrates in the Australasian Region....

  13. Pengendalian Nematoda Puru Akar (Meloidogyne spp. pada Buncis dengan Bakteri Pasteuria penetrans dan Solarisasi

    Directory of Open Access Journals (Sweden)

    B. Triman

    2001-07-01

    Full Text Available The objectives of the research were to study the effect of P. penetrans and soil solarization on the population of root-knot nematodes (Meloidogyne spp. and the effect of soil solarization on the infectivity of P. penetrans. The research was done in the field with high population of plant parasitic nematode especially root-knot nematodes. Soil solarization was done in dry season by covering the soil before french beans (buncis were planted with transparent plastic and P. penetrans were inoculated before soil solarization. Factorial design in Completely Randomized Design was used in this experiment with the following factors: 1 soil solarization (within 1, 2, and 3 moths; 2 isolates of P. penetrans (i.e. isolate 2 and 3. The research results were: 1 Isolate 2 and 3 of P. penetrans were able to parasitize root-knot nematodes in soil solarized within 1, 2, and 3 months; 2 the length of soil solarization afected the infectivity of P. penetrans on Meloidogyne spp. The percentages of Meloidogyne spp. infected with isolate 2 of P. penetrans in soil solarization within 1, 2, and 3 months were 40.3%; 25.7%, and 10.1%, respectively, whereas in soil inoculated with isolate 3 of P. penetrans were: 37.3%, 10.2%, and 2.2%, respectively; 3 inoculation of P. penetrans reduced the root damage caused by root-knot nematodes (Meloidogyne spp.; and 4 treatment of P. penetrans combined with soil solarization reduced the root damage caused by root-knot nematodes (Meloidogyne spp.. Key words: Pasteuria penetrans, soil solarization, root-knot nematode

  14. [The morphology and differential diagnosis of parasitic larvae of Triodontophorus (Nematoda, Strongylidae)].

    Science.gov (United States)

    Kharchenko, V A; Dvoĭnos, G M

    1989-01-01

    356 parasitic larvae of the genus Triodontophorus from Equidae (two Equus hemionus and one E. grevyi) have been investigated. They belong to three phenons, which differ from each other by the shape and dimensions of a stoma, the structure of teeth and other signs. That phenons belong to three different species: T. serratus, T. tenuicollis and T. brevicauda. The differential diagnosis of L4 of that species of Triodontophorus are given.

  15. Systematic studies on the genera Leptosomatum Bastian, 1865 and Leptosomatides Filipjev, 1918 (Nematoda: Leptosomatidae)

    NARCIS (Netherlands)

    Bongers, A.M.T.

    1984-01-01

    Leptosomatum bacillatum, a marine nematode that occurs in high densities in the sponge Halichondria panicea, has an annual reproductive cycle at Dutch latitudes. During the period that the population has been studied, the smallest juveniles were present from July to September. Females continue

  16. New morphological data on Cucullanus pinnai pinnai (Nematoda) parasitizing Pimelodus maculatus (Pimelodidae) in southeastern Brazil.

    Science.gov (United States)

    Vieira, Vivian Suane de Freitas; Vieira, Fabiano Matos; Luque, José Luis

    2015-01-01

    This paper describes the morphology of Cucullanus pinnai pinnai parasitizing Pimelodus maculatus in the Guandu River, Brazil, based on differential interference contrast (DIC) microscopy and scanning electron microscopy (SEM), providing new morphological data about this species of parasite. Nematodes were collected between May and October 2012 from specimens of Pimelodus maculatus in the Guandu River (22°48'2"S, 43°37'35"W), in the state of Rio de Janeiro, Brazil. Some characteristics of specimens of Cucullanus in this study fall within the range of morphological variations of previously studied C. pinnai pinnai. Most of the specimens studied here had excretory pore and deirids located at the posterior end of the oesophagus, a feature not recorded in previous studies of this species. In addition, the size of the gubernaculum was larger than the other specimens previously studied. The SEM and DIC analyses of C. pinnai revealed several morphological details of the cephalic region and the tail papillae. With regard to the polymorphism of C. pinnai, morphological and genetic studies of this cucullanid nematode are needed, involving large numbers of host species and a wide geographical distribution.

  17. Neoalloionema indicum n.sp. (Nematoda: Alloinematidae), a new alloionematid from India

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk

    2016-01-01

    Roč. 18, č. 8 (2016), s. 949-962 ISSN 1388-5545 Institutional support: RVO:60077344 Keywords : Alloionema appendiculatum * Neoalloionema tricaudatum * molecular Subject RIV: EG - Zoology Impact factor: 1.162, year: 2016

  18. Phasmarhabditis apuliae n. sp. (Nematoda: Rhabditidae), a new rhabditid nematode from milacid slugs

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk

    2016-01-01

    Roč. 18, č. 9 (2016), s. 1095-1112 ISSN 1388-5545 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : bionomics * description * Italy Subject RIV: EG - Zoology Impact factor: 1.162, year: 2016

  19. Phasmarhabditis bonaquaense n. sp. (Nematoda: Rhabditidae), a new slug-parasitic nematode from the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mekete, T.; Mráček, Zdeněk

    2016-01-01

    Roč. 4179, č. 3 (2016), s. 530-546 ISSN 1175-5326 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : Phasmarhabditis * Pellioditis * ITS rDNA Subject RIV: EH - Ecology, Behaviour Impact factor: 0.972, year: 2016

  20. Morphological and molecular characterisation of the entomoparasitic nematode Hammerschmidtiella diesingi (Nematoda, Oxyurida, Thelastomatidae).

    Science.gov (United States)

    Blanco, María V; Lax, Paola; Dueñas, Juan C Rondan; Gardenal, Cristina N; Doucet, Marcelo E

    2012-09-01

    A population of Hammerschmidtiella diesingi was detected in specimens of Periplaneta americana collected in Córdoba city (Argentina) was studied. Nematodes were characterized based on morphological, morphometric and genetic (D2-D3 expansion segment) analyses. New data are provided, especially on particular morphological characters that were a matter of controversy in previous studies. H. diesingi is reported in Argentina for the first time.

  1. Eulimdana clava (Nematoda: Filarioidea) infection in domestic pigeons (Columba livia domestica): Molecular characterization and pathological changes.

    Science.gov (United States)

    Šupić, Jovana; Alić, Alma Šeho; Hasanić, Melida; Goletić, Šejla; Duscher, Georg G; Hodžić, Adnan; Alić, Amer

    2018-02-15

    Filarial nematodes of the Eulimdana genus inhabit subcutaneous tissue of various avian species, mostly Charadriiforme birds. In domestic pigeons, E. clava is the only species recorded in the subcutaneous tissue in a number of isolated cases. In the present study, we discuss the morphology and histopathology of filarial nematodes recovered from subcutaneous tissue of domestic pigeons in Bosnia and Herzegovina. In total 110 pigeons were submitted to necropsy at the Department of Pathology of the Faculty of Veterinary Medicine in Sarajevo. At necropsy, in four pigeons (3.6%) numerous thread-like 0.9-2.1 cm long nematode parasites were observed in the subcutaneous tissue, peritracheal and periesophageal connective tissue. In one pigeon, the parasites were also found free in the body cavity around the heart and lungs. In addition, several 80-90 μm long microfilariae were noted in the tissue cross-sections. No significant lesions were observed associated with adult parasites or microfilariae. Based on morphology, host species and localization detected parasites were identified as E. clava. Molecular analyses of the cox1 and 12S rRNA nucleotide sequences herein generated revealed the close genetic relationship to other filarioid nematodes. The importance of the nematodes in pigeons and the lack of sequences in genetic databases for comparison of avian filarial parasites are emphasized. Copyright © 2018 Elsevier B.V. All rights reserved.

  2. Three species of entomopathogenic Nematodes of the family Steinernematidae (Nematoda: Rhabditida) new to continental Portugal

    Czech Academy of Sciences Publication Activity Database

    Valadas, V.; Mráček, Zdeněk; Oliveira, S.; Mota, M.

    2011-01-01

    Roč. 39, č. 2 (2011), s. 169-178 ISSN 0391-9749 Grant - others:Fundacao para a Ciencia e a Tecnologia (PT) SFHR/BD/22086/2005 Institutional research plan: CEZ:AV0Z50070508 Keywords : Steinernema intermedium * S. kraussei * Steinernema sp.

  3. Two new species of philometrids (Nematoda: Philometridae) from marine fishes off South Carolina

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; de Buron, I.

    2009-01-01

    Roč. 95, č. 3 (2009), s. 722-727 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Philometroides * marine fish Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.195, year: 2009

  4. Potensi Bakteri Endofit Pengendali Nematoda Peluka Akar (Pratylenchus brachyurus pada Nilam

    Directory of Open Access Journals (Sweden)

    RITA HARNI

    2007-03-01

    Full Text Available Root lesion nematode (Pratylenchus brachyurus is one of the most important pathogens of patchouli that caused significant losses. Studies on the potential of endophytic bacterial to control P. brachyurus on patchouli had been conducted. To evaluate the effectiveness of endophytic bacterial against to P. brachyurus on patchouli, nine isolates of bacteria (NJ2, NJ25, NJ41, NJ46, NJ57, NA22, ERB21, ES32, and E26 were applied by deeping root seedling into bacterial suspension. A study of the physiological characteristics of nine isolates was conducted by using specific medium. The results showed that endophytic bacterial was significantly reduced the population of P. brachyurus and all isolates bacterial promoted growth of patchouli (shoot weight, root weight, and root length. Four isolates, i.e. Bacillus NJ46, Bacillus Na22, Bacillus NJ2, and Bacillus NJ57 were among the potential control agents that reduced nematode populations as much as 68.1–73.9%. Almost all of the isolated bacteria from patchouli roots were able to solubilizing phosphate, while some of them had the ability to produce chitinase, cellulase, protease, HCN, and fluorescency.

  5. Mononchoides composticola n. sp. (Nematoda:Diplogastridae) associated with composting processes: morphological, molecular and autecological characterisation

    NARCIS (Netherlands)

    Steel, H.; Moens, T.; Scholaert, A.; Boshoff, M.; Houthoofd, W.; Bert, W.

    2011-01-01

    Mononchoides composticola n. sp. was isolated from compost and is described based on light and scanning electron microscopy, supplemented with SSU rDNA sequence data. It is characterised by the following features: a denticulate ridge in addition to the dorsal claw-like tooth, a small tooth-like

  6. Uncinaria hamiltoni (Nematoda: Ancylostomatidae) in South American Sea Lions, Otaria flavescens, From Northern Patagonia, Argentina

    OpenAIRE

    Berón-Vera, B.; Crespo, Enrique Alberto; Raga Esteve, Juan Antonio; Pedraza, S. N.

    2004-01-01

    Thirty-one South American sea lion pups (Otaria flavescens) found dead in Punta León, Argentina, during the summer of 2002, were examined for hookworms (Uncinaria hamiltoni). Parasite parameters were analyzed in 2 locations of the rookery, i.e., a traditional, well-structured breeding area and an expanding area with juveniles and a lax social structure. Prevalence of hookworms was 50% in both localities, and no difference was observed in prevalence between pup sexes (P > 0.05). Hookworms were...

  7. Uncinaria hamiltoni (Nematoda: Ancylostomatidae) in South American sea lions, Otaria flavescens, from northern Patagonia, Argentina.

    Science.gov (United States)

    Berón-Vera, B; Crespo, E A; Raga, J A; Pedraza, S N

    2004-08-01

    Thirty-one South American sea lion pups (Otaria flavescens) found dead in Punta León, Argentina, during the summer of 2002, were examined for hookworms (Uncinaria hamiltoni). Parasite parameters were analyzed in 2 locations of the rookery, i.e., a traditional, well-structured breeding area and an expanding area with juveniles and a lax social structure. Prevalence of hookworms was 50% in both localities, and no difference was observed in prevalence between pup sexes (P > 0.05). Hookworms were concentrated in the small intestine. Transmammary transmission is assumed because only adult hookworms were found in the pups. The mean intensity of hookworms per pup was 135; the mean intensity in females (92.78) was significantly different (P 0.05) in intensity was found between the 2 breeding areas, although prevalence was higher in the traditional breeding area than in the other area. Location was the only factor affecting hookworm prevalence (P log-linear model: 0.9552; chi2: 1.5629). No apparent trend between body condition and intensity of hookworms was observed.

  8. Photomicrographic images of some features of Uncinaria spp (Nematoda: Ancylostomatidae) from otariid pinnipeds.

    Science.gov (United States)

    Lyons, E T; DeLong, R L

    2005-03-01

    Photomicrographs of several morphologic features of hookworms (Uncinaria spp) from northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups are presented. The main purpose is to show and describe some physical characteristics of hookworms from the two hosts; it is not to decide from these attributes whether the Uncinaria spp are the same species. The number of species of Uncinaria in pinnipeds is uncertain and specimens need to be examined from the various infected seals and sea lions before the taxonomy of these parasites can be clarified. Information in the present paper should aid in this determination.

  9. Toxocara cati (Nematoda, Ascarididae in different wild feline species in Brazil: new host records

    Directory of Open Access Journals (Sweden)

    Moisés Gallas

    2013-05-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2013v26n3p117 This is the first detailed description of Toxocara cati parasitizing felines in South America. Seventeen run over wild felines (Leopardus colocolo, Leopardus geoffroyi, Leopardus tigrinus, and Puma yagouaroundi were collected from different towns in the State of Rio Grande do Sul, Brazil. The morphometry of males and females allowed the identification of specimens as being T. cati. The helminths were found in the stomach and intestine of hosts with prevalences of 66.6% in L. colocolo, L. geoffroyi, and L. tigrinus; and 60% in P. yagouaroundi. The ecological parameters were calculated for each host and L. colocolo had the highest infection intensity (22.5 helminths/ host. This is the first report of T. cati parasitizing four wild felines species in southern Brazil, besides a new record of this parasite for two host species.

  10. Toxocara cati (Schrank, 1788 (Nematoda, Ascarididae in different wild feline species in Brazil: new host records

    Directory of Open Access Journals (Sweden)

    Moisés Gallas

    2013-09-01

    Full Text Available This is the first detailed description of Toxocara cati parasitizing felines in South America. Seventeen run over wild felines (Leopardus colocolo, Leopardus geoffroyi, Leopardus tigrinus, and Puma yagouaroundi were collected from different towns in the State of Rio Grande do Sul, Brazil. The morphometry of males and females allowed the identification of specimens as being T. cati. The helminths were found in the stomach and intestine of hosts with prevalences of 66.6% in L. colocolo, L. geoffroyi, and L. tigrinus; and 60% in P. yagouaroundi. The ecological parameters were calculated for each host and L. colocolo had the highest infection intensity (22.5 helminths/host. This is the first report of T. cati parasitizing four wild felines species in southern Brazil, besides a new record of this parasite for two host species.

  11. Toxocara cati (Nematoda: Ascarididae in Didelphis albiventris (Marsupialia: Didelphidae from Brazil: a case of pseudoparasitism

    Directory of Open Access Journals (Sweden)

    Hudson Alves Pinto

    2014-12-01

    Full Text Available Eggs of Toxocara cati were found in the feces of Didelphis albiventris from a peridomestic urban environment in Brazil. Negative fecal tests following short-term captivity of the opossums, as well as the absence of ascaridids during necropsy, suggest the occurrence of pseudoparasitism. Implications of the findings for the epidemiology of toxocariasis are discussed.

  12. The first report of Cosmocerca parva (Nematoda: Cosmocercidae from Colostethus fraterdanieli (Anura: Dendrobatidae in Colombia

    Directory of Open Access Journals (Sweden)

    Sandra M. Sánchez

    2010-12-01

    Full Text Available We discovered the nematode parasite, Cosmocerca parva, in C. fraterdanieli; this is the first record of this nematode species inColombia and C. fraterdanieli is a novel host of C. parva.

  13. Dracunculus brasiliensis sp. n. (Nematoda: Dracunculidae) from the anaconda, Eunectes murinus (Ophidia: Boidae)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Santos, C. P.

    2009-01-01

    Roč. 104, č. 3 (2009), s. 589-592 ISSN 0932-0113 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Dracunculus * Eunectes * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.721, year: 2009

  14. New species of Freitascapillaria (Nematoda: Capillariidae) from the intestine of Cottus caeruleomentum (Teleostei: Cottidae) in Maryland

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Muzzall, P.

    2009-01-01

    Roč. 95, č. 4 (2009), s. 987-990 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Freitascapillaria * Cottus * USA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.195, year: 2009

  15. Identifikasi Nematoda Sista Kuning (Globodera rostochiensis pada Kentang di Batu, Jawa Timur

    Directory of Open Access Journals (Sweden)

    Mulyadi Mulyadi

    2003-07-01

    Full Text Available Identification of Globodera rostochiensis was done based on the information given by the Direktorat Perlindungan Hortikultura, that the potato plants in the sub district of Bumiaji, Batu, East Java, were attacked by potato cyst nematode (Globodera. Plants and soil samples were taken from the potato's areas in the sub-district of Bumiaji, especially in the villages of Brakseng (± 1,700-1,800 m a.s.l, Tunggangan (± 1,600-1,700 m a.s.l, Kembangan (± 1,600-1.700 m a.s.l, and Junggo (± 1,200 m a.s.l. Based on morphological observations on eggs, larvae, females, and cysts, the nematode was identified as Globodera rostochiensis with special morphological characters: 1 second stage larvae vermifonn with total body length 531 - 563μ (x 548,4 μ, body width 22 - 26 μ (x 23,6u, stylet well developed with stylet knobs rounded, and part of the posterior portion hyaline in appearance; 2 cyst globular in shape with protruding neck, cyst's length 470 - 1,008 μ (x 638,08 μ, cyst's width 357 - 744 μ (x 490,33μ, and when the vulval basin is lost forming a single circular fenestra. Cuticle surface between anus and vulva basin have more than 12 paralel ridges.

  16. MEKANISME KETAHANAN KENTANG (SOLANUM TUBEROSUM TERHADAP NEMATODA SISTA KUNING (GLOBODERA ROSTOCHIENSIS

    Directory of Open Access Journals (Sweden)

    Dewi Fitriyanti, Mulyadi, dan Christanti Sumardiyono .

    2011-11-01

    Full Text Available Resistance mechanism of potato (Solanum tuberosum to golden cyst nematode (Globodera rostochiensis.  The research was conducted from June 2005 to May 2006 in Kepuhharjo, Cangkringan, Sleman, Yogyakarta. The aims of the research were to find the resistance status of 20 potato varieties, role of  chlorogenic acid and caffeic acid  in the resistance mechanism and histopathological differences between resistant and susceptible potato varieties to  G. rostochiensis. The results showed that two varieties were resistant to G. rostochiensis, i.e, Hertha and Manohara.  Thirteen varieties were moderately resistant, i.e; No.30, No.44, No. 5, No.19, Batang Hitam, Agria,  Desiree, Berolina, Atlantik, No.095, Cipanas, FLS and Colombus, and five varieties were susceptible, i.e; Kikondo, Granola, Erika, LBR, and Fries. The presence of chlorogenic acid and caffeic acid in Hertha (resistant variety and in Granola (susceptible variety were analized by TLC. In Hertha chlorogenic acid was detected at 4, 5, and 6 days after inoculation (DAI, whereas in Granola it was detected at 1 and 6 DAI. It was assumed that the presence of chlorogenic acid for 3 successively week in Hertha, caused it more resistant than Granola.  Cell lignification inhibited                       G. rostochiensis grow well inside the root tissue of Hertha, whereas in Granola only a few cell was lignified as indicated by less absorption of red colour (safranin in infected areas. Caffeic acid was not detected either in uninoculated or inoculated of both potato varieties.  It indicated that caffeic acid might not be naturally present in Hertha and Granola and nematode infection could not stimulate the production of this compound.

  17. Primer registro de Serratospiculum tendo (Nematoda: Diplotriaenidae para el Perú

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    Luis A. Gomez-Puerta

    2014-05-01

    Full Text Available Reportamos por primera vez la presencia del nematodo, Serratospiculum tendo Nitzsch, 1819, parasitando los sacos aéreos de un halcón peregrino (Falco peregrinus Tunstall, 1771. Seis nematodos (2 machos y 4 hembras fueron colectados e identificados como S. tendo. El hallazgo de este nematodo constituye el primer registro en el Perú.

  18. Influence of water infusion of medicinal plants on larvae of Strongyloides papillosus (Nematoda, Strongyloididae

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    A. A. Boyko

    2016-08-01

    Full Text Available One of the most common nematodes of ruminants is Strongyloides papillosus (Wedl, 1856. Disease caused by these parasites brings economic losses to livestock operations. Therefore it is necessary to control their numbers. The eggs and three larval stages of S. papillosus live in the environment, while the fourth, fifth and mature individuals live in host organisms. Control of these parasites is necessary at all stages of development, including the free-living stage. An experiment on changes in the number strongiloids under the influence of environmental factors was carried out using aqueous extracts of medicinal plants. In the laboratory experiment we researched the effect on the survival of invasive and noninvasive types of S. papillosus larvae of 24 hours exposure at different doses to Artemisia absinthium Linnaeus, 1753, Artemisia annua Linnaeus, 1753, Echinacea purpurea (Linnaeus, 1753 Moench, 1794, Matricaria chamomilla Linnaeus, 1753, Tanacetum vulgare Linnaeus, 1753, Salvia sclarea Linnaeus, 1753, Levisticum officinale W.D.J. Koch, 1824, Petroselinum crispum (Miller, 1768 Nyman ex A.W. Hill, 1925. The death of 50% of S. papillosus invasive larvae was registered at 464 ± 192 mg/l concentration of aqueous extract of S. sclarea inflorescences. The greatest effect up-on the non-invasive larvae was caused by aqueous extracts of inflorescences of S. sclarea, M. chamomilla and seeds of P. crispum: at concentrations of 327 ± 186, 384 ± 155 and 935 ± 218 mg/l, respectively, 50% of non-invasive larvae died. According to the results of the research, we suggest further study of the nematocidal activity of combinations, contained in the aboveground parts, of clary sage (S. sclarea, camomile (M. chamomilla and seeds of parsley (P. crispum, and also experimental usage of these species in the fodder compound for cattle, sheep, goats and pigs on experimental farms.

  19. A new atractid nematode, Atractis vidali sp. n. (Nematoda: Atractidae), from cichlid fishes in southern Mexico

    Czech Academy of Sciences Publication Activity Database

    González-Solís, D.; Moravec, František

    2002-01-01

    Roč. 49, č. 3 (2002), s. 227-230 ISSN 0015-5683 R&D Projects: GA AV ČR IAA6022901 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematode * freshwater fishes * Atractis vidali Subject RIV: EG - Zoology Impact factor: 0.515, year: 2002

  20. A new species of Parodontophora (Nematoda: Axonolaimidae) from the intertidal zone of the East China Sea

    Science.gov (United States)

    Wang, Haixia; Huang, Yong

    2016-02-01

    This study described a new species of free-living nematode discovered in the intertidal mudflat of Ximen Island, East China Sea. The new species, designated Parodontophora longiamphidata sp. nov., was characterized by a cylindrical body with tapering extremeties; cuticle smooth without somatic setae; four short cephalic setae; cylindrical buccal cavity with six clawlike teeth at the top of stoma; pharynx cylindrical with widened base; amphidial fovea crook-shaped with elongated scalariform branch extending past level of base of pharynx and ventral gland; ventral gland cell long-oval shaped located posterior to pharyngo-intestinal junction; excretory pore at level of middle of buccal cavity; tail conico-cylindrical with enlarged tip; three caudal gland cells, male spicules arched with cephalic proximal end and tapered distal end; gubernaculum with dorso-caudal apophysis; female with two opposed outstretched ovaries; and vulva at slightly post-midpoint of body length. This new species was close to P. wuleidaowanensis Zhang, 2005 and P. polita Gerlach, 1955 in terms of long amphidial fovea branch. The newly found species was easily distinguishable from the two documented; its amphidial fovea branch (255-290 µm versus 72-106 and 125-150 µm) was obviously longer. Key to the Parodontophora species with a longer amphidial fovea branch was given.

  1. Schulzia chiribita n. sp. (Nematoda, Trichostrongylina, Molineoidea parasite of Leptodactylus rhodonotus (Amphibian from Peru

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    Durette-Desset M.C.

    2000-03-01

    Full Text Available A third species of the genus Schulzia Travassos, 1937 a parasite of Leptodactylus rhodonotus (Amphibian, Leptodactylidae originating from Peru is described. By the pattern of the caudal bursa, the specimens are closely related to the two other species. They are distinguished from Schulzia uzu Lent & Santos, 1989, parasite from Atelopus oxyrhynchus in Venezuela, by the shape of the ovejector and from Schulzia travassosi Durette-Desset, Baker & Vaucher, 1985, parasite from Bufo crucifer in Brasil, Bufo granulosus and Leptodactylus bufonius in Paraguay, by the shape of the spicules. The presence of a new species in Peru points out the wide geographic distribution of the genus in the Neotropical region.

  2. A new species of Cheiropteronema (Nematoda, Molineidae) in Artibeus planirostris (Chiroptera, Phyllostomidae) from Argentina

    OpenAIRE

    Oviedo, Mirna C; Ramallo, Geraldine; Claps, Lucía E

    2010-01-01

    Se describe a Cheiropteronema striatum sp. nov. parásita del murciélago Artibeus planirostris (Spix, 1823). Se caracteriza por presentar vesícula cefálica estriada, superficie de los huevos con puntuaciones, espículas iguales, nacimiento simétrico del ala espicular, particular disposición y número de crestas del synlophe y por carecer de diente esofágico. Es la segunda especie del género y la primera que se registra en quirópteros frugívoros de la Argentina.Cheiropteronema striatum sp. nov. i...

  3. Identification of Nematodirus species (Nematoda: Molineidae) from wild ruminants in Italy using ribosomal DNA markers.

    Science.gov (United States)

    Gasser, R B; Rossi, L; Zhu, X

    1999-11-01

    The sequence of the second internal transcribed spacer of ribosomal DNA was determined for four species of Nematodirus (Nematodirus rupicaprae, Nematodirus oiratianus, Nematodirus davtiani alpinus and Nematodirus europaeus) from roe deer or alpine chamois. The second internal transcribed spacer of the four species varied in length from 228 to 236 bp, and the G + C contents ranged from 41 to 44%. While no intraspecific sequence variation was detected among multiple samples representing three of the taxa, sequence differences of 5.9-9.7% were detected among the four species, Nematodirus davtiani alpinus and N. rupicaprae were genetically most similar (94.1%), followed by N. oiratianus, N. europaeus and N. rupicaprae (91.1-91.5%), whereas N. oiratianus was genetically most different from N. davtiani alpinus. The interspecific sequence differences were exploited for the delineation of the four species by PCR-based restriction fragment length polymorphism (using two enzymes) and single-strand conformation polymorphism. The results have implications for diagnosis, epidemiology and for studying the systematics of the Nematodirinae.

  4. New species of Oswaldocruzia (Nematoda: Molineidae) in Ameiva festiva (Squamata: Teiidae) from Nicaragua.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R; Vitt, Laurie J

    2006-04-01

    Oswaldocruzia nicaraguensis n. sp. (Strongylida: Molineidae) from the intestines of Ameiva festiva (Sauria: Teiidae) is described and illustrated. Oswaldocruzia nicaraguensis represents the 78th species assigned to the genus and is most similar to the Caribbean species of the genus by possessing spicules in which each of the 3 divisions terminates in numerous fine points. Of the 8 species assigned to this group, O. nicaraguensis is most similar to Oswaldocruzia moraveci; of the 8 species, only O. moraveci and O. nicaraguensis possess a type II bursa and lack cervical alae. It is separated from O. moraveci by the position of the tips of ribs 5-6; close together in O. nicaraguensis, well separated in O. moraveci.

  5. The phylogenetic relationships of endemic Australasian trichostrongylin families (Nematoda: Strongylida) parasitic in marsupials and monotremes.

    Science.gov (United States)

    Chilton, Neil B; Huby-Chilton, Florence; Koehler, Anson V; Gasser, Robin B; Beveridge, Ian

    2015-10-01

    The phylogenetic relationships of the endemic (or largely endemic) Australasian trichostrongylin nematode families Herpetostrongylidae, Mackerrastrongylidae and Nicollinidae as well as endemic trichostrongylin nematodes currently placed in the families Trichostrongylidae and Molineidae were examined using the complete large subunit (28S) ribosomal RNA gene. The Herpetostrongylinae proved to be monophyletic. However, representatives of the Nicollinidae nested with the Herpetostrongylinae. The Mackerrastrongylidae was also a monophyletic group and included Peramelistrongylus, currently classified within the Trichostrongylidae. The Globocephaloidinae, currently considered to be a subfamily of the Herpetostrongylidae, was excluded from the family in the current analysis. Ollulanus and Libyostrongylus, included for the first time in a molecular phylogenetic analysis, were placed within the Trichostrongylidae. This study provided strong support for the Herpetostrongylidae (including within it the Nicollinidae, but excluding the Globocephaloidinae) and the Mackerrastrongylidae as monophyletic assemblages. Additional studies are required to resolve the relationships of the remaining endemic Australasian trichostrongylin genera.

  6. Lamanema chavezi (Nematoda: Molineidae): epidemiological data of the infection in South American camelids of Northwest Argentina.

    Science.gov (United States)

    Cafrune, M M; Marín, R E; Rigalt, F A; Romero, S R; Aguirre, D H

    2009-12-23

    Faecal samples from llamas (n=708), vicuñas (n=171) and guanacos (n=4) were obtained between December 2004 and May 2009 in three Provinces of Northwest Argentina (Jujuy, Salta and Catamarca) to know the distribution, prevalence and intensity of Lamanema chavezi infection in these South American camelid species (SACs). Faeces were examined by a sedimentation-flotation technique using a Cl(2)Zn+ClNa solution (specific gravity=1.59). Eggs of L. chavezi occurred in 30.3% of 89 llama herds and in 18.5% of 708 llamas sampled with a mean intensity of 271.8 eggs/g (EPG) of faeces (range 20-2120). The highest values for all parameters of the infection were registered in llamas from Catamarca Province. Significant differences (P<0.001, Fisher's exact test) were detected only for the lower prevalence in llamas from Jujuy respect to those from the other two Provinces. The overall individual prevalence of L. chavezi in llamas was lower than in reports from adult domestic camelids of neighbour countries while mean intensity was higher. The individual prevalence of L. chavezi in guanacos was 75.0%, with a mean intensity of 66.0 EPG (range 40-120) while no vicuñas were detected as infected. Most of infected SACs were located at the phytogeographical region of Andean Patagonic Domain with a dispersion ranging between 22 degrees 10' and 26 degrees 40' South latitude.

  7. Differences in the second internal transcribed spacer of four species of Nematodirus (Nematoda: Molineidae).

    Science.gov (United States)

    Newton, L A; Chilton, N B; Beveridge, I; Gasser, R B

    1998-02-01

    Genetic differences among Nematodirus spathiger, Nematodirus filicollis, Nematodirus helvetianus and Nematodirus battus in the nucleotide sequence of the second internal transcribed spacer (ITS-2) of ribosomal DNA ranged from 3.9 to 24.7%. Pairwise comparisons of their ITS-2 sequences indicated that the most genetically similar species were N. spathiger and N. helvetianus. N. battus was the most genetically distinct species, with differences ranging from 22.8 to 24.7% with respect to the other three species. Some of the nucleotide differences among species provided different endonuclease restriction sites that could be used in restriction fragment length polymorphism studies. The ITS-2 sequence data may prove useful in studies of the systematics of molineid nematodes.

  8. New species of Schulzia (Nematoda: Molineidae) in Ptychoglossus festae (Squamata: Gymnophthalmidae) from Panama.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R; Telford, Sam R

    2006-10-01

    Schulzia ptychoglossi n. sp. (Strongylida: Molineidae) from the intestines of Ptychoglossus festae (Squamata: Gymnophthalmidae) is described and illustrated. Schulzia ptychoglossi n. sp. represents the fourth species assigned to the genus and is most similar to the Venezuelan species S. usu by possessing a cervical inflation that begins a short distance from the anterior end of the body. Schulzia ptychoglossi differs from S. usu in that ray 8 separates midway between the root and tip of the dorsal ray in S. ptychoglossi, but separates close to the root of the dorsal ray in S. usu.

  9. A new species of Oswaldocruzia (Molineidae: Nematoda) in Chaunus marinus (Amphibian: Bufonidae) (Linneaus, 1758) from Brazil.

    Science.gov (United States)

    Santos, Jeannie N; Giese, Elane G; Maldonado, Arnaldo Júnior; Lanfredi, Reinalda M

    2008-02-01

    Oswaldocruzia belenensis n. sp. (Strongylida: Molineidae) from the small intestine of Chaunus marinus (L.) from Belém, Pará State, Brazil is described and illustrated by light and scanning electron microscopy. Oswaldocruzia belenensis n. sp. is a neotropical species of this genus, harboring caudal bursa Type II, spicules divided in 3 branches, i.e., a blade, shoe, and fork. The blade is divided in 4 points, of which at least 2 are bifurcated. Cervical alae are absent; there is a simple cephalic vesicle and synlophe with low ridges perpendicular to the body without chitinous supports. The most closely related species are O. bonsi and O. lescurei. Oswaldocruzia belenensis n. sp. differs from O. lescurei and O. bonsi by the number and location of cephalic papillae, rays 2-3 and 5-6 running parallel and slightly separated, ray 6 not overlapping ray 8, and body structure morphometry. Oswaldocruzia belenensis n sp. also differs from O. lescurei by the discontinuity of the longitudinal ridges, the number of subdivisions of the blade, and the absence of extra processes at the bifurcation level of the fork of the spicules. The new species differs from O. bonsi by male and female body dimensions, the symmetry of the caudal bursa, dimension and subdivisions of the spicules without extra processes of the fork, 2 extra processes at the distal division of the blade, and location of ray 7 at the anterior margin of the cloacal aperture. Oswaldocruzia belenensis n. sp. represents the 82nd species assigned to the genus.

  10. Una especie nueva de Cheiropteronema (Nematoda, Molineidae) en Artibeus planirostris (Chiroptera, Phyllostomidae) en la Argentina

    OpenAIRE

    Oviedo,Mirna C; Ramallo,Geraldine; Claps,Lucía E

    2010-01-01

    Se describe a Cheiropteronema striatum sp. nov. parásita del murciélago Artibeus planirostris (Spix, 1823). Se caracteriza por presentar vesícula cefálica estriada, superficie de los huevos con puntuaciones, espículas iguales, nacimiento simétrico del ala espicular, particular disposición y número de crestas del synlophe y por carecer de diente esofágico. Es la segunda especie del género y la primera que se registra en quirópteros frugívoros de la Argentina.

  11. Two new species of Oswaldocruzia (Nematoda, Molineidae) parasitising lizards in Ukraine.

    Science.gov (United States)

    Svitin, Roman

    2017-05-09

    Two new species, Oswaldocruzia lisnykiensis sp. n. and Oswaldocruzia lacertica sp. n., are described from Anguis fragilis L. (Reptilia: Anquidae) and Lacerta agilis L. (Reptilia: Lacertidae) respectively. Both species belong to the Palaearctic group of species which have spicules divided in three main branches (blade, fork and shoe) with fork division above its distal third. The new species differs from all previously known Palaearctic species by several morphological characters: type of caudal bursa (type III in O. lisnykiensis and type I in O. lacertica), shape of spicules (blade distally divided into four tips and specific shape of shoe) and synlophe structure (narrow cervical alae consist of three crests in both species). Illustrated descriptions of O. lisnykiensis and O. lacertica based on 63 and 38 specimens respectively are presented. Host specificity and geographical distribution of the species are discussed. O. dispar is considered as a species inquirenda.

  12. The Enterobiinae subfam. Nov. (Nematoda, Oxyurida) pinworm parasites of primates and rodents.

    Science.gov (United States)

    Hugot, J P; Gardner, S L; Morand, S

    1996-02-01

    Recent redescriptions of most members of the Oxyuridae Cobbold, 1864 parasitic in primates revealed that they share following derived characters: sexual dimorphism of lateral alae (single-crested in the males, double-crested in the females); in males a second pair of genital papillae always surrounded by strongly cuticularized rings; in females, uterine tube divided into 2 parts by a cellular wall forming a diaphragm. These characters are interpreted as synapomorphies, providing evidence that these taxa represent a monophyletic group, and we propose to classify them in a new subfamily of the Oxyuridae: the Enterobiinae subfam.nov. The Enterobiinae as recognized herein occurs in both Old World and New World Primates and rodents of the family Sciuridae (tribe Sciurini in the Holarctic region and tribe Xerini in the Ethiopian region). The new subfamily includes the following genera: Enterobius Leach, 1853; Colobenterobius Quentin, Betterton & Krishnasamy, 1979; Rodentoxyuris Quentin & Tenora 1974; Xeroxyuris Hugot, 1995; Lemuricola Chabaud & Petter, 1959; Protenterobius Inglis, 1961; Madoxyuris Chabaud, Brygoo & Petter, 1965; Trypanoxyuris Vevers, 1923; Hapaloxyuris Inglis & Cosgrove, 1965 and Paraoxyuronema Artigas, 1936. The genus Paraoxyuronema is revalidated as a subgenus of Trypanoxyuris due to its specialized buccal structures. This genus groups all pinworm nematodes specific for primates of the family Atelidae, including: P. brachytelesi Artigas, 1937 occurring in Brachyteles arachnoides; P. atelis (Cameron, 1929) occurring in Ateles spp., and P. duplicidens (Buckley, 1931) and P. lagothricis (Buckley, 1931), which are parasites of Lagothrix spp. Inglisoxyuris inglisi Chabaud, Petter & Golvan, 1961, included in the monospecific genus Inglisoxyuris and previously classified as a subgenus of the Lemuricola, does not share the characters of the new subfamily and, until its precise classification can be considered with more information, it is proposed to refer to this species as an Oxyuridae sensu lato. A diagnosis and a key of the genera included in the new subfamily are given.

  13. Contracaecumovale (Nematoda: Anisakidae) from Rollandia rolland Quoy & Gaimard 1824 (Aves, Podicipedidae) in Argentina.

    Science.gov (United States)

    Galeano, Noelia Adelina; Tanzola, Ruben Daniel

    2012-01-01

    Necropsy on 15 specimens of white-tufted grebe, Rollandiarolland, caught in the Mar Chiquita and Chascomús lagoons (Buenos Aires province), revealed the presence of Contracaecumovale (Linstow, 1907). This nematode shows a marked specificity for podicipediform birds. The specimens were identified from morphological study on features such as cephalic and esophageal structures and caudal papillae, using both optical and scanning electron microscopy. This is the first record of C. ovale parasitizing R. rolland in Argentina.

  14. Uji Patogenisitas Bakteri Pasteuria Penetrans terhadap Nematoda Puru Akar (Meloidogyne spp.

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    Mulyadi Mulyadi

    1996-12-01

    nematodes (Meloidogyne spp. in micro plot and in the field. The pathogenicity study in micro plot was done in Banguntapan, and field experiment was done in Ngipiksari, Pakem, Steman, Yogyakarta. The plots were arranged in Randomized Completely Block Design with three replications. The treatments used in the research were: isolat no. 2 and 3 of P. penetrans; carbofuran nematicide. and control. The results in micro plot test were: 1 root damage caused by root-knot nematodes in plot treated with P. penetrans lower than the control, and 2 percentages of parasitism of P. penetrans were 63.57 % (in isolate no. 2 and 53.46 % (in isolate no. 3. In field experiment the results showed: 1 P. penetrans found to be effective in reducing root damage caused by Meloidogyne spp. especially in 45 days old of tomato plant, whereas in 90 days old the effectiveness were decreased; 2 P. penetrans was able to grow and reproduction in the field: 3 the level of parasitisms of P. penetrans were increased rapidly during the experiment: and 4 the highest yield was found in tomato treated with carbofuran and followed by isolate 2, isolate 3, and control.

  15. Fish as paratenic hosts of Serpinema trispinosum (Leidy, 1852) (Nematoda: Camallanidae).

    Science.gov (United States)

    Moravec, F; Mendoza-Franco, E; Vivas-Rodríguez, C

    1998-04-01

    Third-stage larvae of the nematode Serpinema trispinosum (Leidy, 1852) were collected from the intestine of the freshwater cichlid, Cichlasoma urophthalmus (Günther), from a small lake in Yucatan, Mexico. This is the first record of Serpinema larvae from fishes, and their presence may reflect the importance of fishes as paratenic hosts of turtle parasites in this genus.

  16. Experimental infection of Contracaecum multipapillatum (Nematoda: Anisakinae) from Mexico in the domestic cat.

    Science.gov (United States)

    Vidal-Martínez, V M; Osorio-Sarabia, D; Overstreet, R M

    1994-08-01

    Juveniles of Contracaecum multipapillatum infected the Mayan cichlid (Cichlasoma urophthalmus) and adults infected the olivaceous cormorant (Phalacrocorax olivaceus) and the great egret (Casmerodius albus) in the coastal lagoon at Celestun, State of Yucatan, Mexico. All are new host records, and, even though the geographic locality record of Mexico for the species has not been published, unidentified but presumably conspecific specimens have been reported from there. When juveniles of C. multipapillatum were fed to a kitten, but not rats, ducks, or chickens, they developed into adults. Measurements and morphological data are provided on the specimens from the kitten. Development of an avian ascaridoid in the intestine of a mammal increases the potential of this widespread species to infect other mammals, including humans.

  17. First description of the males of .i.Aphelenchoides limberi./i. Steiner, 1936 (Nematoda: Aphelenchina)

    Czech Academy of Sciences Publication Activity Database

    Čermák, V.; Háněl, Ladislav; Gaar, V.; Douda, O.

    2012-01-01

    Roč. 49, č. 3 (2012), s. 181-186 ISSN 0440-6605 Institutional research plan: CEZ:AV0Z60660521 Keywords : nematode * parthenogenesis * amphimixis Subject RIV: EH - Ecology, Behaviour Impact factor: 0.783, year: 2012

  18. Efficacy of sulfuryl fluoride against the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchidae), in Pinus pinaster boards.

    Science.gov (United States)

    Bonifácio, Luís F; Sousa, Edmundo; Naves, Pedro; Inácio, Maria L; Henriques, Joana; Mota, Manuel; Barbosa, Pedro; Drinkall, Mike J; Buckley, Stanislas

    2014-01-01

    The pinewood nematode (PWN) Bursaphelenchus xylophilus is an important conifer disease worldwide. It is the direct cause of the death of millions of pines in south-east Asia (mainly Japan, China and Korea) and has been established in Portugal since 1999. The phasing out of methyl bromide has created an urgent need for alternative treatment of wood packaging materials. The effect of sulfuryl fluoride (SF), a broad-spectrum fumigant used to control insects, was tested in Pinus pinaster boards naturally infested by PWN. Boards were fumigated for 24 h at three different temperatures (15, 20 and 30 °C) with dosage ranges of 3169-4407, 1901-4051 and 1385-2141 gh m(-3) respectively. Treated wood was sampled for nematode identification and counting, before treatment and after 24 h, 72 h and 21 days. No survival was found in the 15 °C and 30 °C treatments, while at 20 °C the mortality ranged from 94.06 to 100%. Some reasons for the survival at 20 °C are presented. Results confirm SF to be an effective quarantine treatment for PWN at 15 and 30 °C. Further studies are needed to obtain the most effective dosage at 20 °C, and to determine the toxicity of SF fumigation on B. xylophilus at other temperatures, especially at 25 °C. © 2013 Society of Chemical Industry.

  19. Cosmopolitanism and Biogeography of the Genus Manganonema (Nematoda: Monhysterida in the Deep Sea

    Directory of Open Access Journals (Sweden)

    Roberto Danovaro

    2011-09-01

    Full Text Available Spatial patterns of species diversity provide information about the mechanisms that regulate biodiversity and are important for setting conservation priorities. Present knowledge of the biogeography of meiofauna in the deep sea is scarce. This investigation focuses on the distribution of the deep-sea nematode genus Manganonema, which is typically extremely rare in deep-sea sediment samples. Forty-four specimens of eight different species of this genus were recorded from different Atlantic and Mediterranean regions. Four out of the eight species encountered are new to science. We report here that this genus is widespread both in the Atlantic and in the Mediterranean Sea. These new findings together with literature information indicate that Manganonema is a cosmopolitan genus, inhabiting a variety of deep-sea habitats and oceans. Manganonema shows the highest diversity at water depths >4,000 m. Our data, therefore, indicate that this is preferentially an abyssal genus that is able, at the same time, to colonize specific habitats at depths shallower than 1,000 m. The analysis of the distribution of the genus Manganonema indicates the presence of large differences in dispersal strategies among different species, ranging from locally endemic to cosmopolitan. Lacking meroplanktonic larvae and having limited dispersal ability due to their small size, it has been hypothesized that nematodes have limited dispersal potential. However, the investigated deep-sea nematodes were present across different oceans covering macro-scale distances. Among the possible explanations (hydrological conditions, geographical and geological pathways, long-term processes, specific historical events, their apparent preference of colonizing highly hydrodynamic systems, could suggest that these infaunal organisms are transported by means of deep-sea benthic storms and turbidity currents over long distances.

  20. Transcriptomic analysis of the rice white tip nematode, Aphelenchoides besseyi (Nematoda: Aphelenchoididae.

    Directory of Open Access Journals (Sweden)

    Feng Wang

    Full Text Available BACKGROUND: The rice white tip nematode Aphelenchoides besseyi, a devastating nematode whose genome has not been sequenced, is distributed widely throughout almost all the rice-growing regions of the world. The aims of the present study were to define the transcriptome of A. besseyi and to identify parasite-related, mortality-related or host resistance-overcoming genes in this nematode. METHODOLOGY AND PRINCIPAL FINDINGS: Using Solexa/Illumina sequencing, we profiled the transcriptome of mixed-stage populations of A. besseyi. A total of 51,270 transcripts without gaps were produced based on high-quality clean reads. Of all the A. besseyi transcripts, 9,132 KEGG Orthology assignments were annotated. Carbohydrate-active enzymes of glycoside hydrolases (GHs, glycosyltransferases (GTs, carbohydrate esterases (CEs and carbohydrate-binding modules (CBMs were identified. The presence of the A. besseyi GH45 cellulase gene was verified by in situ hybridization. Given that 13 unique A. besseyi potential effector genes were identified from 41 candidate effector homologs, further studies of these homologs are merited. Finally, comparative analyses were conducted between A. besseyi contigs and Caenorhabditis elegans genes to look for orthologs of RNAi phenotypes, neuropeptides and peptidases. CONCLUSIONS AND SIGNIFICANCE: The present results provide comprehensive insight into the genetic makeup of A. besseyi. Many of this species' genes are parasite related, nematode mortality-related or necessary to overcome host resistance. The generated transcriptome dataset of A. besseyi reported here lays the foundation for further studies of the molecular mechanisms related to parasitism and facilitates the development of new control strategies for this species.

  1. Thelastoma gueyei sp. n. (Nematoda: Thelastomatidae) from the Senegal diplopod Archispirostreptus tumuliporus (Diplopoda: Spirostreptidae)

    Czech Academy of Sciences Publication Activity Database

    Koubková, B.; Baruš, Vlastimil; Matějusová, I.; Hodová, I.; Koubek, Petr

    2006-01-01

    Roč. 8, č. 5 (2006), s. 739-747 ISSN 1388-5545 R&D Projects: GA AV ČR(CZ) IAA6093403 Institutional research plan: CEZ:AV0Z60930519 Keywords : DNA barcoding * Senegal * long-tailed thelastomatids Subject RIV: EG - Zoology Impact factor: 0.722, year: 2006

  2. ASPEK ZOONOTIK PARASIT NEMATODA PADA KERA DAN BINATANG MENGERAT DI BENGKULU, SUMATERA. INDONESIA

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    Untung S.

    2012-09-01

    Full Text Available Twentyfive monkeys and 481 rats were examined for parasitic nematodes in Bengkulu, nine species of nematode were found infecting these animals. Five of filarían nematodes, i.e. Brugia malayi, Brugia pahangi, Dirofilaria magnilarvatum and Edesonfilaria malayensis were infecting monkeys and one speciesTBreinlia booliati, was found infecting rats. Three species of gastrointestinal helminths, i.e. Trichuris trichiura, Enterobius vermicularis and Oestophagomomum spp were found in monkeys; a lung worm, Angiostrongylus cantonensis, was found in rats. The most important nematode species is B. malayi, which was found in Presbytis cristatus (36.8 % and in Macaca fascicularis (20.0 %. T. trichiura was found in R. cristatus (47.9 % and A. cantonensis in Rattus argentiventer (4.0 % and Rattus tiomanicus (2.9%.

  3. KETAHANAN BEBERAPA VARIETAS UNGGUL KEDELAI TERHADAP NEMATODA PURU AKAR (Meloidogyne incognita

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    Wayan Sukanaya

    2001-07-01

    Full Text Available The aims of this experiment are to find the varieties of soybean which are resistant against root-knot nematode (M. incognita and to determine its attractancy toward the roots of resistant and susceptible soybean varieties. The experiment was divided into two parts, both parts using Completely Randomized Design. The first part was designed to determine the resistance and the second one to determine attractancy. The experiments were conducted in a green house and Plant Pest and Disease Laboratory, Faculty of Agriculture, Udayana University for 5 months. Of the 23 varieties of soybean tested against M. incognita showed that two varieties (Petek and Rinjani were moderately resistant, 11 varieties were moderately susceptible and 10 varieties were found to be susceptible. However, none of variety was resistant. While on attractancy of root test of moderately resistant and susceptible variety it was found that M. incognita was more attracted to root of susceptible variety (Ringgit than to moderately resistant variety (Petek. Key words: soybean, resistance, root-knot nematode

  4. Effect of ES products from Anisakis (Nematoda: Anisakidae) on experimentally induced colitis in adult zebrafish

    DEFF Research Database (Denmark)

    Haarder, Simon; Kania, Per Walter; Holm, Thomas

    2017-01-01

    Inflammatory bowel disease (IBD) in developed countries is linked with elevated hygienic standards. One of the several factors involved in this question may be reduced exposure to the immunomodulatory effects of parasitic helminths. Several investigations on treatment of mice and humans with helm...

  5. Two atypical new species of the genus Sectonema Thorne, 1930 (Nematoda, Dorylaimida, Aporcelaimidae from Vietnam

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    Thi Anh Duong Nguyen

    2016-01-01

    Full Text Available Two new species of the genus Sectonema from natural habitats of northern Vietnam are studied. This paper includes their descriptions, measurements, line illustrations, and light microscope (LM and scanning electron microscope (SEM pictures. Sectonema tropicum sp. nov. is characterized by a 2.56–3.24 mm long body, 19–21 μm broad lip region, odontostyle 20–21 μm long at its ventral side, 730–834 μm long neck, pharyngeal expansion occupying 52–59% of total neck length, uterus a simple tube-like structure 150–242 μm long or 1.2–2.5 times the body diameter, pars refringens vaginae present, V = 48–52, short (31–40 μm, c = 70–91, c’ = 0.5–0.6 and rounded tail, 91–97 μm long spicules, and only one weakly developed ventromedian supplement. Sectonema vietnamense sp. nov. is characterized by its slender (a = 33–49 and 2.71–4.25 mm long body, 14–16 μm broad lip region, odontostyle 8–9 μm long at its ventral side, 716–918 μm long neck, pharyngeal expansion occupying 63–67% of total neck length, uterus simple and 209–242 μm long or 2.5–2.9 times the corresponding body diameter, pars refringens vaginae absent, V = 54, short (34–39 μm, c = 70–115, c’ = 0.6–0.8 and rounded tail, 59–75 μm long spicules, and three or four irregularly spaced ventromedian supplements bearing hiatus. Both species are also characterized by their nearly continuous lip region, an atypical feature in this genus. Molecular analysis of S. tropicum sp. nov. confirms that Sectonema is a natural (monophyletic taxon, very close to Metaporcelaimus.

  6. Unveiling the oxidative metabolism of Achatina fulica (Mollusca: Gastropoda) experimentally infected to Angiostrongylus cantonensis (Nematoda: Metastrongylidae).

    Science.gov (United States)

    Tunholi-Alves, Vinícius Menezes; Tunholi, Victor Menezes; Garcia, Juberlan; Mota, Esther Maria; Castro, Rosane Nora; Pontes, Emerson Guedes; Pinheiro, Jairo

    2018-06-01

    For the first time, alterations in the oxidative metabolism of Achatina fulica experimentally infected with different parasite loads of Angiostrongylus cantonensis were determined. For this, the hemolymph activities of lactate dehydrogenase (LDH) and hexokinase and the glucose concentrations in the hemolymph, as well as the polysaccharide reserves in the digestive gland and cephalopedal mass, were assessed. Additionally, the contents of some carboxylic acids in the hemolymph of infected and uninfected snails were determined by high-performance liquid chromatography (HPLC), permitting a better understanding of the alterations related to the host's oxidative metabolism. As the main results, activation of oxidative pathways, such as the glycolytic pathway, was demonstrated in response to the increase in the activity of hexokinase. This tendency was confirmed by the decrease in the contents of glucose in the hemolymph of parasitized snails, indicating that the infection by A. cantonensis alters the host's metabolism, and that these changes are strongly influenced by the parasite load. This metabolic scenario was accompanied by activation of the anaerobic fermentative metabolism, indicated not only by an increase in the activity of (LDH), but also by a reduction of the content of pyruvic acid and accumulation of lactic acid in the hemolymph of parasitized snails. In this circumstance, maintenance of the host's redox balance occurs through activation of the fermentative pathways, and LDH plays a central role in this process. Together, the results indicate that A. cantonensis infection induces activation of the anaerobic metabolism of A. fulica, characterized not only by the accumulation of lactic acid, but also by a reduction in the pyruvic acid and oxalic acid contents in the hemolymph of the infected snails.

  7. First record of molluscs naturally infected with Angiostrongylus cantonensis (Chen, 1935 (Nematoda: Metastrongylidae in Brazil

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    Roberta Lima Caldeira

    2007-11-01

    Full Text Available Seeking the identification of Angiostrongylus cantonensis as a potential etiological agent of three clinical cases of eosinophilic meningitis, mollusc specimens were collected in the state of Espírito Santo, Brazil. The snails were identified as Sarasinula marginata (45 specimens, Subulina octona (157, Achatina fulica (45 and Bradybaena similaris (23. Larvae obtained were submitted to polymerase chain reaction and restriction fragment length polymorphism diagnosis. Their genetic profile were corresponded to A. cantonensis. Rattus norvegicus experimentally infected with third-stage larvae, developed menigoencephalitis, and parasites became sexually mature in the lungs. Additionally, larvae obtained from A. fulica snails, from São Vicente, state of São Paulo, also showed genetic profiles of this nematode. This is the first record of Brazilian molluscs infected with this nematode species.

  8. Steinernema poinari sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Mráček, Zdeněk; Půža, Vladimír; Nermuť, Jiří

    2014-01-01

    Roč. 3760, č. 3 (2014), s. 336-350 ISSN 1175-5326 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : new species * affine/intermedium group * ITS Subject RIV: EG - Zoology Impact factor: 0.906, year: 2014

  9. Identifikasi karakter morfologi nematoda sista pada tanaman jagung (Heterodera zeae di Indonesia

    Directory of Open Access Journals (Sweden)

    Yuliantoro Baliadi

    2012-02-01

    Full Text Available Identification of nematode species is the fi rst step in the resolution of major nematological problems. Accurate identification of maize cyst nematode species is essential for more detailed research, particularly for it control measure. Although morphological observations by using the key characters of each nematode are suitable for identification purposes, the cyst nematodes could not beclearly identified based on it due to the wide variations. For the identification of the cyst nematode species, key characters relating to body surface, head, mouth, esophagus, tail as well as habitat and feeding habits was used in the present examination. Procedures for the permanent and temporary preparations of nematodes were used. The nematode specimens were observed under the microscope to confirm the key characters. Results of measurements and descriptions revealed that the cyst nematode collected from maize roots in Madura island have the same ambifenestrate as that of Heterodera zeae. The underbridge is short and thin and there is two layer of bullae arrangement in vulval cone, the first layer with four finger-like bullae located immediately below the underbridge and the second layer very randomly located below the fi rst one. These characteristics are of H. zeae. These complete of morphological identifications are a new report of H. zeae in Indonesia.

  10. New Cyst Nematode, Heterodera sojae n. sp. (Nematoda: Heteroderidae) from Soybean in Korea.

    Science.gov (United States)

    Kang, Heonil; Eun, Geun; Ha, Jihye; Kim, Yongchul; Park, Namsook; Kim, Donggeun; Choi, Insoo

    2016-12-01

    A new soybean cyst nematode Heterodera sojae n. sp. was found from the roots of soybean plants in Korea. Cysts of H. sojae n. sp. appeared more round, shining, and darker than that of H. glycines . Morphologically, H. sojae n. sp. differed from H. glycines by fenestra length (23.5-54.2 µm vs. 30-70 µm), vulval silt length (9.0-24.4 µm vs. 43-60 µm), tail length of J2 (54.3-74.8 µm vs. 40-61 µm), and hyaline part of J2 (32.6-46.3 µm vs. 20-30 µm). It is distinguished from H. elachista by larger cyst (513.4-778.3 µm × 343.4-567.1 µm vs. 350-560 µm × 250-450 µm) and longer stylet length of J2 (23.8-25.3 µm vs. 17-19 µm). Molecular analysis of rRNA large subunit (LSU) D2-D3 segments and ITS gene sequence shows that H. sojae n. sp. is more close to rice cyst nematode H. elachista than H. glycines . Heterodera sojae n. sp. was widely distributed in Korea. It was found from soybean fields of all three provinces sampled.

  11. Rhabdias lacertae n. sp. (Nematoda: Rhabdiasidae), the first rhabdiasid species parasitising lizards in Europe

    Czech Academy of Sciences Publication Activity Database

    Moravec, František

    2010-01-01

    Roč. 77, č. 1 (2010), s. 23-27 ISSN 0165-5752 Institutional research plan: CEZ:AV0Z60220518 Keywords : Rhabdias * Lacerta * Slovakia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.056, year: 2010

  12. Swedish Plectida (Nematoda). Part 4. The genus Leptolaimus de Man, 1876.

    Science.gov (United States)

    Holovachov, Oleksandr; Boström, Sven

    2013-11-25

    Twelve known and nine new species of Leptolaimus are described from bottom sediments collected in marine habitats of Sweden, including the Bothnian Sea and Bothnian Bay, the Baltic Sea proper, Gullmarn Fjord and the Skagerrak. Three of these species have been previously recorded in Sweden while nine are new records for the Swedish fauna. The following known species are redescribed: Leptolaimus papilliger de Man, 1876, L. cupulatus Lorenzen, 1972, L. danicus Jensen, 1978, L. donsi (Allgén, 1946) comb. n., L. mixtus Lorenzen, 1972, L. pellucidus (Southern, 1914) comb. n., L. venustus Lorenzen, 1972, L. lorenzeni (Boucher & de Bovée, 1972) comb. n., L. alatus Vitiello, 1971, L. macer Lorenzen, 1972, L. septempapillatus Platt, 1973, L. elegans (Schuurmans Stekhoven & De Coninck, 1933) Gerlach, 1958. Leptolaimus primus sp. n. is characterised by the 319-472 µm long body; rounded labial region continuous with body contour; cephalic setae 1.5-2.0 µm long; amphid located 7.0-11.5 µm from anterior end; first body pore located 18.5-28.0 µm from anterior end; lateral field originating 35 µm from anterior end; female without supplements, vagina without pars refringens, vulva midventral; male without tubular and with four alveolar supplements, alveolar supplements without sclerotized inner ring; spicules arcuate and 13.5-16.0 µm long. Leptolaimus secundus sp. n. is characterised by the 576-645 µm long body; rounded labial region continuous with body contour; cephalic setae 2.0 µm long; amphid located 6.5-7.0 µm from anterior end; first body pore located 23.0-28.5 µm from anterior end; lateral field originating 18.0-23.0 µm from anterior end; female without supplements, vagina without pars refringens, vulva midventral; male with single tubular and 9-15 alveolar supplements, tubular supplement weakly arcuate with blunt tips, alveolar supplements with sclerotized lining; spicules arcuate and 23.0-26.5 µm long. Leptolaimus tertius sp. n. is characterised by the 576-579 µm long body; rounded labial region continuous with body contour; cephalic setae 2 µm long; amphid located 4-5 µm from anterior end; first body pore located 21.0-23.5 µm from anterior end; lateral field originating 26-29 µm from anterior end; male with four tubular and 7-10 alveolar supplements, tubular supplements weakly arcuate with blunt tips, alveolar supplements with sclerotized inner ring; spicules arcuate and 25-26 µm long. Leptolaimus quartus sp. n. is characterised by the 597-686 mm long body; rounded labial region continuous with body contour; cephalic setae 2.0-3.5 µm long; amphid located 5.0-7.0 µm from anterior end; first body pore located 23.5-27.0 µm from anterior end; lateral field originating 19.0-27.0 µm from anterior end; male with three tubular and 8-9 alveolar supplements, tubular supplements straight with blunt expanded tips, alveolar supplements with sclerotized inner ring; spicules arcuate and 23.0-25.0 µm long. Leptolaimus quintus sp. n. is characterised by the 443-528 µm long body; rounded labial region continuous with body contour; cephalic setae 1.0-2.0 µm long; amphid located 7.0-10.0 µm from anterior end; first body pore located 18.0-28.5 µm from anterior end; lateral field originating 25.0-41.0 µm from anterior end; female with two tubular supplements (one just posterior to cardia and one in front of anus), vagina without pars refringens, vulva right-sublateral; male with 8-10 tubular and without alveolar supplements, tubular supplements weakly arcuate with anchor-like tips; spicules arcuate and 17.0-22.0 µm long. Leptolaimus sextus sp. n. is characterised by the 626-728 µm long body; truncated labial region offset from body contour; cephalic setae 1.5-2.0 µm long; amphid located 8.0-12.0 µm from anterior end; first body pore located 41.0-48.5 µm from anterior end; lateral field originating 28.0-41.0 µm from anterior end; female without supplements, vagina without pars refringens, vulva midventral; male with five (rarely six) tubular and without alveolar supplements, tubular supplements weakly S-shaped with bifid tips; spicules arcuate and 39.0-46.0 µm long. Leptolaimus septimus sp. n. is characterised by the 679-850 µm long body; truncate labial region offset from body contour; cephalic setae 2.5-3.5 µm long; amphid located 8.5-11.5 µm from anterior end; first body pore located 37.0-44.0 µm from anterior end; lateral field originating 26.5-37.0 µm from anterior end; female without supplements, vagina without pars refringens, vulva midventral; male with four (rarely five) tubular and without alveolar supplements, tubular supplements weakly S-shaped, with bifid or blunt tips; spicules arcuate and 31.0-33.5 µm long. Leptolaimus octavus sp. n. is characterised by the 541-638 µm long body; truncate labial region continuous with body contour; cephalic setae 1.5-2.0 µm long; amphid located 8.5-12.0 µm from anterior end; first body pore located 31.5-41.0 µm from anterior end; lateral field originating 26.0-40.0 µm from anterior end and expanding into bursa-like structures along the tail; female without supplements, vagina without pars refringens, vulva midventral; male with four tubular and without alveolar supplements, tubular supplements weakly S-shaped with dentate tips; spicules arcuate and 30.0-31.5 µm long. Leptolaimus nonus sp. n. is characterised by the 403-633 µm long body; rounded labial region continuous with body contour; cephalic setae 1.5-3.0 µm long; amphid located 8.0-10.0 µm from anterior end; first body pore located 16.5-29.0 µm from anterior end; lateral field originating 47.0-99.0 µm from anterior end and expanding into bursa-like structures along the proximal part of the tail; female without supplements, vagina with bacilliform pars refringens, vulva right-subventral; male with 4-5 tubular and without alveolar supplements, tubular supplements weakly arcuate with dentate tips; spicules arcuate and 17.0-26.0 µm long. The following nomenclatorial changes are proposed: Eutelolaimus donsi Allgén, 1947 is transferred to the genus Leptolaimus, as L. donsi (Allgén, 1947) comb. n.; Halaphanolaimus cangionensis Gagarin & Nguyen Vu Thanh, 2007 is transferred to the genus Leptolaimus, as L. cangionensis (Gagarin & Nguyen Vu Thanh, 2007) comb. n.; Halaphanolaimus harpaga Boucher & de Bovée, 1972 is transferred to the genus Leptolaimus, as L. harpaga (Boucher & de Bovée, 1972) comb. n.; Halaphanolaimus lorenzeni Boucher & de Bovée, 1972 is transferred to the genus Leptolaimus, as L. lorenzeni (Boucher & de Bovée, 1972) comb. n.; Halaphanolaimus pellucidus Southern, 1914 is transferred to the genus Leptolaimus, as L. pellucidus (Southern, 1914) comb. n.; Halaphanolaimus rivalis Gagarin & Nguyen Vu Thanh, 2007 is transferred to the genus Leptolaimus, as L. rivalis (Gagarin & Nguyen Vu Thanh, 2007) comb. n.; Halaphanolaimus marinus Kamran, Nasira & Shahina, 2010 is transferred to the genus Leptolaimus as L. marinus (Kamran, Nasira & Shahina, 2010) comb. n. and considered a junior synonym of Leptolaimus rivalis (Gagarin & Nguyen Vu Thanh, 2007) comb. n.; Halaphanolaimus sergeevae Ürkmez & Brennan, 2013 is transferred to the genus Leptolaimus as L. sergeevae (Ürkmez & Brennan, 2013) comb. n.; Leptolaimus vitielloi nom. nov. is proposed for Leptolaimus minutus Vitiello, 1971 nec L. minutus (Schuurmans Stekhoven, 1942) comb. n.; Polylaimium exile Cobb, 1920 is transferred to the genus Leptolaimus, as L. exilis (Cobb, 1920) comb. n. and is considered species inquirendae. A taxonomic review, tabular compendium and identification key for species of the genus Leptolaimus are also given. 

  13. Swedish Plectida (Nematoda). Part 10. The genus Deontolaimus de Man, 1880.

    Science.gov (United States)

    Holovachov, Oleksandr; Boström, Sven

    2015-10-28

    The genus Deontolaimus de Man, 1880 is revised and the genus Camacolaimus de Man, 1889 is considered a junior synonym of Deontolaimus based on re-examination of type material of Camacolaimus tardus de Man, 1889 and C. barbatus Warwick, 1970. Two known and three new species of Deontolaimus are described from bottom sediments collected in marine habitats of Sweden: Deontolaimus uniformis (Cobb, 1920) comb. n., D. longicauda (de Man, 1922) comb. n., Deontolaimus catalinae sp. n., D. paraguillei sp. n. and Deontolaimus timmi sp. n. Deontolaimus catalinae sp. n. is characterized by body length of 1.3-1.7 mm; anterior-most somatic sensilla located short distance posterior to amphid; cephalic sensilla equal to 0.2 labial region diameter in length; amphidial fovea ventrally-unispiral with one turn, located in front of cephalic sensilla bases; excretory pore located short distance posterior to onchiostyle base; onchiostyle with bluntly rounded tip and subcylindrical body; male with alveolar supplements extending from anterior end to middle of body, tubular supplements absent; spicules 36-40 µm long; and didelphic female reproductive system. Deontolaimus paraguillei sp. n. is characterized by body length of 1.4-1.8 mm; anterior-most somatic sensilla located at level with onchiostyle; cephalic sensilla equal to 0.2-0.3 labial region diameter in length; amphidial fovea ventrally-unispiral with one turn, located at level with cephalic sensilla bases; excretory pore located just posterior to nerve ring level; onchiostyle with bluntly rounded tip and subcylindrical body; male with alveolar supplements extending from anterior end to about three body diameters in front of cloaca, tubular supplements absent; spicules 42-46 µm long; and didelphic female reproductive system. Deontolaimus timmi sp. n. is characterized by body length of 0.7-0.9 mm; anterior-most somatic sensilla located at level with onchiostyle; cephalic sensilla equal to 0.2-0.3 labial region diameter in length; amphidial fovea ventrally-unispiral with one turn, located just in front of cephalic sensilla bases; excretory pore located just posterior to nerve ring level; onchiostyle with triangular tip with bluntly rounded apex and strongly sclerotized dorsal edge, and subcylindrical body; male with alveolar supplements extending from anterior end to anterior part of intestine, tubular supplements absent; spicules 28 µm long; and didelphic female reproductive system. The following nomenclatorial changes are proposed: genera Acontiolaimus Filipjev, 1918, Camacolaimoides De Coninck & Schuurmans Stekhoven, 1933, Camacolaimus de Man, 1889, Digitonchus Cobb, 1920 and Ypsilon Cobb, 1920 are synonimized with the genus Deontolaimus de Man, 1880; Camacolaimus reykjanesi De Coninck, 1943 and Camacolaimus glauxicola Allgén, 1951a are considered junior synonyms of Deontolaimus papillatus de Man, 1880; Camacolaimus barbatus apud Pastor de Ward, 1984 is described as the separate species Deontolaimus catalinae sp. n.; Camacolaimus tardus apud Lorenzen, 1969 is considered to be the separate species Deontolaimus lorenzeni nom. n.; Camacolaimus tardus apud Timm, 1963 is described as the separate species Deontolaimus timmi sp. n.; Camacolaimus barbatus Warwick, 1970 is considered a junior synonym of Deontolaimus tardus (de Man, 1889) comb. n.; Camacolaimus parvus Timm, 1961 is transferred to the genus Deontolaimus as D. parvus (Timm, 1961) comb. n.; Digitonchus cylindricaudatus Chitwood, 1951 is transferred to the genus Deontolaimus as D. cylindricaudatus (Chitwood, 1951) comb. n.; Ypsilon exile Cobb, 1920 is transferred to the genus Deontolaimus as D. exilis (Cobb, 1920) comb. n.; Camacolaimus guillei de Bovee, 1977 is transferred to the genus Deontolaimus as D. guillei (de Bovee, 1977) comb. n.; Camacolaimus longicauda de Man, 1922 is transferred to the genus Deontolaimus as D. longicauda (de Man, 1922) comb. n.; Camacolaimus monhystera Gerlach, 1967 is transferred to the genus Deontolaimus as D. monhystera (Gerlach, 1967) comb. n.; Camacolaimus pontollittoralis Uzunov, 1977 is transferred to the genus Deontolaimus as D. pontollittoralis (Uzunov, 1977) comb. n.; Camacolaimus praedator de Man, 1922 is transferred to the genus Deontolaimus as D. praedator (de Man, 1922) comb. n.; Camacolaimus prytherchi Chitwood, 1935 is transferred to the genus Deontolaimus as D. prytherchi (Chitwood, 1935) comb. n.; Camacolaimus tardus de Man, 1889 is transferred to the genus Deontolaimus as D. tardus (de Man, 1889) comb. n.; Camacolaimus trituberculatus Blome, 1982 is transferred to the genus Deontolaimus as D. trituberculatus (Blome, 1982) comb. n.; and Digitonchus uniformis Cobb, 1920 is transferred to the genus Deontolaimus as D. uniformis (Cobb, 1920) comb. n. A taxonomic review and identification key for species of the genus Deontolaimus are also given.

  14. Redescription of Oncophora melanocephala (Rudolphi, 1819 Baudin-Laurencin, 1971 (Nematoda, Camallanidae

    Directory of Open Access Journals (Sweden)

    R. Magalhães Pinto

    1988-06-01

    Full Text Available Oncophora melanocephala, a camallanid nematode from the small intestine of Priacanthus arenatus Cuvier, 1829 is redescribed from the Coast of Rio de Janeiro State, Brazil and this occurrence represents a new host record for the speciesOncophora melanocephala, um nematóide camalanídeo de instestino delgado de Priacanthus arenatus Cuvier, 1829, é redescrito da Costa do Estado do Rio de Janeiro, Brasil, e esta ocorrência representa um novo hospedeiro para a espécie.

  15. Alloionema californicum n. sp. (Nematoda: Alloionematidae): a new alloionematid from USA

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk; Lewis, E.

    2016-01-01

    Roč. 4184, č. 3 (2016), s. 505-516 ISSN 1175-5326 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : Alloionema appendiculatum * Neoalloionema tricaudatum * ITS rDNA Subject RIV: EG - Zoology Impact factor: 0.972, year: 2016

  16. Temporal and Spatial Distribution of Enterobius vermicularis (Nematoda: Oxyuridae) in the Prehistoric Americas.

    Science.gov (United States)

    Reinhard, Karl J; Araújo, Adauto; Morrow, Johnica J

    2016-10-01

    Investigations of Enterobius sp. infection in prehistory have produced a body of data that can be used to evaluate the geographic distribution of infection through time in the Americas. Regional variations in prevalence are evident. In North America, 119 pinworm positive samples were found in 1,112 samples from 28 sites with a prevalence of 10.7%. Almost all of the positive samples came from agricultural sites. From Brazil, 0 pinworm positive samples were found in 325 samples from 7 sites. For the Andes region, 22 pinworm positive samples were found in 411 samples from 26 sites for a prevalence of 5.3%. Detailed analyses of these data defined several trends. First, preagricultural sites less frequently show evidence of infection compared to agricultural populations. This is especially clear in the data from North America, but is also evident in the data from South America. Second, there is an apparent relationship between the commonality of pinworms in coprolites and the manner of constructing villages. These analyses show that ancient parasitism has substantial value in documenting the range of human behaviors that influence parasitic infections.

  17. Bakteri Endofit Asal Berbagai Akar Tanaman sebagai Agens Pengendali Nematoda Puru Akar Meloidogyne incognita pada Tomat

    Directory of Open Access Journals (Sweden)

    Pradana Pandu Ankardiansyah

    2016-08-01

    Full Text Available Infection caused by root knot nematode (RKN Meloidogyne incognita may cause yield losses. Little is known regarding the effectiveness of endophytic bacterial group as biocontrol agents of RKN. This research was aimed to obtain endophytic bacteria group from 16 species of plants, which effectively controlled the RKN. Isolation of endophytic bacteria group was conducted using NA 20%, NA 50%, TSA 20%, TSA 50%, and King’s B medium. All of the bacteria groups giving negative result in hypersensitive and haemolytic tests, was further examined for their ability to produce protease, chitinase, and cyanide acid. The same endophytic bacteria groups were also tested for their potential to control juvenile 2 of M. incognita on tomatoes by seed treatment and soil drenching. Agronomical and pathological traits were observed 40 days after nematodes infestation. Eighty endophytic bacteria groups were successfully isolated and 17 of them were considered potential. Physiological test showed that 16 groups of endophytic bacteria can produce protease enzyme, 12 groups can produce chitinase enzyme, and 5 groups can produce cyanide acid. Specific endophytic bacteria group, i.e. TmtN5 from roots of tomato plant, is the most effective isolate for suppressing root damage and population of RKN. This group was effective as biocontrol agents of RKN because it produceds chitinase, protease, and cyanide acid. This research provided a new information regarding the potential use of endophytic bacteria group as a biocontrol agent of RKN.

  18. Granular Formulations of Steinernema carpocapsae (strain All) (Nematoda: Rhabditida) with Improved Shelf Life

    Science.gov (United States)

    Connick, W. J.; Nickle, W. R.; Williams, K. S.; Vinyard, B. T.

    1994-01-01

    Shelf life (nematode survival) of Steinernema carpocapsae (strain All) nematodes at 21 C in "Pesta" granules, made by a pasta-like process, was increased from 8 to 26 weeks by incorporating low concentrations of formaldehyde. Pesta samples containing an average of 427,000 nematodes/g were prepared with wheat flour (semolina or bread flour), kaolin, bentonite, peat moss, nematode slurry, and formaldehyde (0-1.4% w/w) and were dried to a water content of 23.6-26.9%. Nematodes emerged from Pesta (S. carpocapsae) granules when placed in water or on moist filter paper. Incorporation of 0.2% w/w formaldehyde (nominal; 0.05% by analysis) was optimum for increasing nematode survival in semolina-based Pesta, and also inhibited fungal growth on the granules. Bread flour Pesta samples prepared by formaldehyde addition to the nematode slurry prior to dough preparation, rather than by addition to a mixture of dry ingredients, had longer shelf life. Nematodes recovered from granules made with 0.2% formaldehyde and stored 20 weeks at 21 C caused 100% mortality of wax moth (Galleria mellonella) larvae. PMID:19279903

  19. Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy

    Czech Academy of Sciences Publication Activity Database

    Půža, Vladimír; Chundelová, Daniela; Nermuť, Jiří; Žurovcová, Martina; Mráček, Zdeněk

    2015-01-01

    Roč. 60, č. 4 (2015), s. 547-554 ISSN 1386-6141 R&D Projects: GA ČR GAP504/12/2352 Grant - others:GA JU(CZ) 052/2013/P Institutional support: RVO:60077344 Keywords : Steinernema * genetic diversity * intra-individual variability Subject RIV: EG - Zoology Impact factor: 1.767, year: 2015 http://link.springer.com/article/10.1007%2Fs10526-015-9664-5

  20. Heterorhabditis sp. (Nematoda: Heterorhabditidae): A Nematode Parasite Isolated from the Banded Cucumber Beetle Diabrotica balteata

    Science.gov (United States)

    Creighton, C. S.; Fassuliotis, G.

    1985-01-01

    A nematode identified as Heterorhabditis sp. was discovered in June 1982 in larval cadavers of the banded cucumber beetle, Diabrotica balteata, in soil on wooded land. Effective beetle control (over 95%) was obtained when larvae were exposed to potted soil containing infective stage nematode juveniles or infected larval cadavers. The nematode was propagated in vivo on larvae of D. balteata, Diaphania nitidalis (the pickleworm), and Galleria mellonella (the greater wax moth). This Heterorhabditis sp. has promising potential as a biocontrol agent for the banded cucumber beetle. PMID:19294074

  1. Pengaruh Penggenangan dan Pengeringan terhadap Populasi dan Siklus Hidup Nematoda Puru Akar Padi (Meloidogyne graminicola

    Directory of Open Access Journals (Sweden)

    Mulyadi Mulyadi

    1997-09-01

    The research results showed: 1 continuously flooding inhibit the penetration and population development of M. graminicola on rice IR 64, but the life cycle of M. graminicola was not affected; and 2 seventy two hours period of draining enhanced the development of M. graminicola compared to period of draining shorter than 72 hours.

  2. Experimental studies on the development of Contracaecum rudolphii (Nematoda: Anisakidae) in copepod and fish paratenic hosts

    Czech Academy of Sciences Publication Activity Database

    Moravec, František

    2009-01-01

    Roč. 56, č. 3 (2009), s. 185-193 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA AV ČR IA62211 Institutional research plan: CEZ:AV0Z60220518 Keywords : Contracaecum rudolphii * life cycle * Czech Republic Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

  3. Steinernema huense sp. n., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Vietnam

    Czech Academy of Sciences Publication Activity Database

    Phan, K. L.; Mráček, Zdeněk; Půža, Vladimír; Nermuť, Jiří; Jarošová, Andrea

    2014-01-01

    Roč. 16, č. 7 (2014), s. 761-775 ISSN 1388-5545 R&D Projects: GA MŠk LH12105 Institutional support: RVO:60077344 Keywords : carpocapsae group * description * molecular Subject RIV: EG - Zoology Impact factor: 1.239, year: 2014

  4. Psittacine birds (Aves : Psittaciformes) as new hosts of Baruscapillaria obsignata (Nematoda : Capillariidae)

    Czech Academy of Sciences Publication Activity Database

    Kajerová, V.; Baruš, Vlastimil

    2005-01-01

    Roč. 74, č. 4 (2005), s. 571-574 ISSN 0001-7213 R&D Projects: GA ČR(CZ) GA524/03/0061 Institutional research plan: CEZ:AV0Z60930519 Keywords : Psittacine birds * capillariids * Czech Republic Subject RIV: EG - Zoology Impact factor: 0.353, year: 2005 http://vfu-www.vfu.cz/acta-vet/vol74/74-571.pdf

  5. Parasitismo natural por Synhimantus (Dispharynx) nasuta (Nematoda: Acuariidae) en Pavo real (Pavo cristatus) en cautiverio

    OpenAIRE

    Gómez-Puerta, Luis A.; Enciso, Marco A.; Rojas, Gianmarco

    2009-01-01

    The nematode Synhimantus (Dispharynx) nasuta (Rudolphi, 1819) Chabaud, 1975 parasiting the proventriculus of two chicks of Common Peafowl (Pavo cristatus Linnaeus, 1758) in captivity from Lima, Peru is described. En el presente trabajo se describe al nematodo Synhimantus (Dispharynx) nasuta (Rudolphi, 1819) Chabaud, 1975 parasitando el proventrículo de dos polluelos de Pavo real (Pavo cristatus Linnaeus, 1758) en cautiverio provenientes de Lima, Perú.

  6. Morphological, molecular and biological characterization of Mehdinema alii (Nematoda: Diplogasterida) from the decorated cricket (Gryllodes sigillatus).

    Science.gov (United States)

    Luong, L T; Platzer, E G; De Ley, P; Thomas, W K

    1999-12-01

    The nematode Mehdinema alii was recovered from the decorated cricket Gryllodes sigillatus (Walker). Morphometric comparisons are presented from 3 populations. The nematode is characterized by dense arrays of spines on the cuticle of the anterior half of the body and a highly elongate, tubular stoma with a dorsal denticle in the glottoid region. Females have a protruding vulva. Young females are amphidelphic, but the anterior ovary disappears in older females bearing multiple developing juveniles. The male is monorchic with asymmetrically placed genital papillae, distally fused spicules, and a highly complex gubernaculum bearing 2 cuticularized thorns that protrude through a separate, postcloacal opening. Adult nematodes are located primarily in the hindgut, whereas juveniles or dauers occur mainly in the genital chamber of both male and female crickets. Male crickets are significantly more likely to be infected than females. This male-biased infection may be linked to the venereal transmission mechanism of the dauers. Although morphologically unusual in many respects, placement of M. alii in Diplogasterida is supported by both the morphology of the anterior digestive tract as well as analysis of its 18S rDNA sequence. These sequence data suggest that M. alii groups most closely with members of the Cylindrocorporidae.

  7. Molecular detection of Setaria tundra (Nematoda: Filarioidea and an unidentified filarial species in mosquitoes in Germany

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    Czajka Christina

    2012-01-01

    Full Text Available Abstract Background Knowledge of the potential vector role of Culicidae mosquitoes in Germany is very scanty, and until recently it was generally assumed that they are not involved in the transmission of anthroponotic or zoonotic pathogens in this country. However, anticipated changes in the course of global warming and globalization may alter their status. Methods We conducted a molecular mass screening of mosquitoes for filarial parasites using mitochondrial 12S rRNA-based real-time PCR. Results No parasites causing disease in humans such as Dirofilaria spp. were detected in about 83,000 mosquitoes tested, which had been collected in 2009 and 2010 in 16 locations throughout Germany. However, minimum infection rates of up to 24 per 1000 mosquitoes were revealed, which could be attributed to mosquito infection with Setaria tundra and a yet unidentified second parasite. Setaria tundra was found to be widespread in southern Germany in various mosquito species, except Culex spp. In contrast, the unidentified filarial species was exclusively found in Culex spp. in northern Baden-Württemberg, and is likely to be a bird parasite. Conclusions Although dirofilariasis appears to be emerging and spreading in Europe, the absence of Dirofilaria spp. or other zoonotic filariae in our sample allows the conclusion that the risk of autochthonous infection in Germany is still very low. Potential vectors of S. tundra in Germany are Ochlerotatus sticticus, Oc. cantans, Aedes vexans and Anopheles claviger. Technically, the synergism between entomologists, virologists and parasitologists, combined with state-of-the-art methods allows a very efficient near-real-time monitoring of a wide spectrum of both human and veterinary pathogens, including new distribution records of parasite species and the incrimination of their potential vectors.

  8. Description and developmental biology of the predatory diplogastrid Acrostichus nudicapitatus (Steiner, 1914 Massey, 1962 (Nematoda: Rhabditida

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    Ahlawat S.

    2016-06-01

    Full Text Available Acrostichus nudicapitatus (Steiner, 1914 Massey, 1962 is redescribed and illustrated along with observations on its developmental biology. Most morphometrics of the present population agree well with those of A. nudicapitatus (Steiner, 1914 Massey, 1962. The largely oviparous females of A. nudicapitatus lay eggs in single-celled or two-celled stage, 1.5 – 2 h after fertilization. In cultured females, the uterine tract was observed to accommodate occasionally as many as 4 – 6 eggs. The eggs are smooth-shelled, oval in shape measuring 45 – 48 x 23 – 26 μm in dimension. The pole of entry of sperm marks the posterior end of the developing embryo. The embryonation time has been recorded to be 20 – 25 h at 25 ± 2 °C. The first moult occurs inside the egg and the juvenile hatches as second stage juvenile. The gonad development follows the trends found in most rhabditids, however, three prime cells of the 12 vulval precursor cells have been observed to be involved in vulva formation.

  9. Philometrids (Nematoda: Philometridae) in carangid and serranid fishes off New Caledonia, including three new species

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.

    2014-01-01

    Roč. 21, MAY 19 2014 (2014), s. 21 ISSN 1252-607X R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Parasitic nematode * Philometra * Marine fish * Alepes * Epinephelus * Selar * New Caledonia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.092, year: 2014

  10. Dioctophyme renale (Nematoda: Enoplida in domestic dogs and cats in the extreme south of Brazil

    Directory of Open Access Journals (Sweden)

    Josaine Cristina da Silva Rappeti

    Full Text Available Abstract Dioctophyme renale is a zoonotic nematode that parasites the kidneys of wild and domestic carnivores, and it has been reported frequently in Brazil. The aim here was to register the number of cases of dogs and cats diagnosed with dioctophymosis by necropsy (1981 to 2014 and ultrasound examination (2010 to 2015 in Pelotas-RS. In this context, a survey was conducted on dioctophymosis cases diagnosed at the Veterinary Pathology Laboratory (LPV and Veterinary Clinical Hospital (HCV of the Federal University of Pelotas (UFPel, and at a specialist veterinary imaging diagnostics clinic. In total, 95 cases were registered. The high series of the disease in dogs can be related to the presence of a large number of stray and semi-domestic dogs in the city, and also due to the ingestion of intermediate hosts of D. renale parasitized with the infective larvae. Thus, it can be concluded that Pelotas is a city with favorable conditions for the occurrence of dioctophymosis with high rate of disease in recent years.

  11. Parasitismo natural por Synhimantus (Dispharynx nasuta (Nematoda: Acuariidae en Pavo real (Pavo cristatus en cautiverio

    Directory of Open Access Journals (Sweden)

    Luis A. Gómez-Puerta

    2011-07-01

    Full Text Available En el presente trabajo se describe al nematodo Synhimantus (Dispharynx nasuta (Rudolphi, 1819 Chabaud, 1975 parasitando el proventrículo de dos polluelos de Pavo real (Pavo cristatus Linnaeus, 1758 en cautiverio provenientes de Lima, Perú.

  12. Human case of gastric infection by a fourth larval stage of Pseudoterranova decipiens (Nematoda, Anisakidae

    Directory of Open Access Journals (Sweden)

    Mercado Rubén

    1997-01-01

    Full Text Available Only three cases of human infection by anisakid nematodes have been reported in Chile since 1976. In the present case, an anisakid worm, identified as a fourth-stage Pseudoterranova decipiens larva, was removed with a gastroendoscopic biopsy clipper from the stomach of a 45 year-old man from southern Chile. The patient, who presented acute epigastric pain and a continuous sensation of having an empty stomach, reported having eaten smoked fish. The worm was fixed in 70% ethanol and cleaned in lactophenol for morphological study. The morphometric characteristics of the worm are described and drawn. Anisakid larvae in fish flesh can be killed by freezing or cooking.

  13. Description of Marylynnia puncticaudata n. sp. (Nematoda, Cyatholaimidae from Bizerte Lagoon, Tunisia

    Directory of Open Access Journals (Sweden)

    Boufahja, F.

    2014-12-01

    Full Text Available A new free–living marine nematode species of Cyatholaimidae, Marylynnia puncticaudata n. sp. from Bizerte Lagoon (Tunisia is morphologically described. Males are characterized by a slightly larger body than females, a cephalic ring followed by ten subcephalic setae, modified cuticular punctuation, caudal lateral differentiation of large dots, and strongly cuticularized gubernaculum with a unique shape and bidenticulated distal half. The cuticle ornamentation of females is similar to the males. However, their caudal lateral differentiation is composed of smaller and more spaced dots. An updated morphological key to species of Marylynnia is given.

  14. Molecular identification of parasitic nematodes (Nematoda: Strongylida) in feces of wild ruminants from Tunisia.

    Science.gov (United States)

    Said, Yousra; Gharbi, Mohamed; Mhadhbi, Moez; Dhibi, Moktar; Lahmar, Samia

    2017-11-08

    In Tunisia and other North African countries, there is a lack of knowledge about parasite biodiversity within threatened wild ruminants and there are not any studies on their gastrointestinal nematodes. Thus the aim of this study was to identify gastrointestinal fauna in the faecal samples of Tunisian wild ruminants. A total of 262 faecal samples were collected from domestic sheep and goat, and wild ruminants (Addax, Barbary sheep, Barbary red deer, Dorcas gazelle, Slender-horned gazelle and Scimitar-horned Oryx) living in protected areas. Samples were examined with floatation (saturated sodium chloride solution), polymerase chain reaction and sequencing of the second internal transcribed spacer region of the rDNA. Microscopic analysis allowed the identification of only Nematodirus genus or molecular tools allowed a first identification of five gastrointestinal nematode species in North African wild ruminants: Chabertia ovina (1.6%), Camelostrongylus mentulatus (1.6%), Marshallagia marshalli (4.7%), Nematodirus helvetianus (62.5%) and Nematodirus spathiger (29.7%). This study reported the first records of C. mentulatus and M. marshalli in Addax and of M. marshalli in Dorcas gazelle and it was the first reported record of N. helvetianus and M. marshalli in Tunisia.

  15. Preferential host switching and codivergence shaped radiation of bark beetle symbionts, nematodes of Micoletzkya (Nematoda: Diplogastridae).

    Science.gov (United States)

    Susoy, V; Herrmann, M

    2014-05-01

    Host-symbiont systems are of particular interest to evolutionary biology because they allow testable inferences of diversification processes while also providing both a historical basis and an ecological context for studies of adaptation. Our investigations of bark beetle symbionts, predatory nematodes of the genus Micoletzkya, have revealed remarkable diversity of the group along with a high level of host specificity. Cophylogenetic analyses suggest that evolution of the nematodes was largely influenced by the evolutionary history of beetles. The diversification of the symbionts, however, could not be attributed to parallel divergence alone; our results indicate that adaptive radiation of the nematodes was shaped by preferential host shifts among closely related beetles along with codivergence. Whereas ecological and geographic isolation have played a major role in the diversification of Micoletzkya at shallow phylogenetic depths, adaptations towards related hosts have played a role in shaping cophylogenetic structure at a larger evolutionary scale. © 2014 The Authors. Journal of Evolutionary Biology © 2014 European Society For Evolutionary Biology.

  16. [Nematodes (Nematoda) from bats (Chiroptera) of the Samarskaya Luka Peninsula (Russia)].

    Science.gov (United States)

    Kirillova, N Iu; Kirillov, A A; Vekhnik, V P

    2008-01-01

    Fauna of parasitic nematodes from Chiroptera of the Samarskaya Luka has been studied. Seven nematode species has been recorded. Numbers of host specimens, indices of extensiveness and intensiveness of the invasion, parasite abundance, and brief characteristics of the nematode species are given. Some nematode species were for the first time recorded in bats of Russia.

  17. Does scavenging extend the host range of entomopathogenic nematodes (Nematoda: Steinernematidae)?

    Science.gov (United States)

    Půza, Vladimír; Mrácek, Zdenĕk

    2010-05-01

    Living and freeze-killed natural and laboratory hosts, with different susceptibility to entomopathogenic nematodes, were exposed to the larvae of Steinernema affine and Steinernema kraussei in two different experimental arenas (Eppendorf tubes, Petri dishes), and the success of the colonisation and eventual progeny production were observed. Both nematodes were able to colonise both living and dead larvae of Galleria mellonella (Lepidoptera) and adult Blatella germanica (Blattodea) even though the progeny production in dead hosts was lower on average. Living carabid beetles, Poecilus cupreus, and elaterid larvae (Coleoptera) were resistant to the infection, however, both nematodes were able to colonise and multiply in several dead P. cupreus and in a majority of dead elaterid larvae. By scavenging, EPNs can utilise cadavers of insects that are naturally resistant to EPN infection, and so broaden their host range. (c) 2010 Elsevier Inc. All rights reserved.

  18. Two new species of free-living marine nematodes (Nematoda: Oncholaimida: Enchelidiidae) from Maemul Island, Korea.

    Science.gov (United States)

    Hong, Jung-Ho; Lee, Wonchoel

    2014-04-04

    Two new species of the family Enchelidiidae Filipjev, 1918 were collected from marine sediments near Maemul Island in South Korea: a new species of Abelbolla Huang & Zhang, 2004 and a new species of Ledovitia Filipjev, 1927. Abelbolla maemulensis sp. nov. is characterized by its small size (1,493 × 38 µm, body length × maximum body diameter); the presence of a circular amphid; the gubernacular apophysis with swollen distal tip; and the complex structure of the gubernaculum. It is close to Abelbolla huanghaiensis Huang & Zhang, 2004, but differs by the structure of gubernacular apophysis and body length (1,493 vs 2,303 µm). Ledovitia brevis sp. nov. can be separated from its congeners by its small size of body, the length of gubernacular apophysis, and the length of the spicules. It is close to Ledovitia pharetrata Wieser, 1953a, but differs by the length of the body (1,699 vs 2,640 µm) and the spicules (40 vs 100 µm).

  19. Molecular characterization and functional analysis of a glutathione peroxidase gene from Aphelenchoides besseyi (Nematoda: Aphelenchoididae).

    Science.gov (United States)

    Wang, Bu-Yong; Wen, Rong-Rong; Ma, Ling

    2017-09-26

    Aphelenchoides besseyi, the nematode agent of rice tip white disease, causes huge economic losses in almost all the rice-growing regions of the world. Glutathione peroxidase (GPx), an esophageal glands secretion protein, plays important roles in the parasitism, immune evasion, reproduction and pathogenesis of many plant-parasitic nematodes (PPNs). Therefore, GPx is a promising target for control A. besseyi. Here, the full-length sequence of the GPx gene from A. besseyi (AbGPx1) was cloned using the rapid amplification of cDNA ends method. The full-length 944 bp AbGPx1 sequence, which contains a 678 bp open reading frame, encodes a 225 amino acid protein. The deduced amino acid sequence of the AbGPxl shares highly homologous with other nematode GPxs, and showed the closest evolutionary relationship with DrGPx. In situ hybridization showed that AbGPx1 was constitutively expressed in the esophageal glands of A. besseyi, suggesting its potential roles in parasitism and reproduction. RNA interference (RNAi) was used to assess the functions of the AbGPx1 gene, and quantitative real-time PCR was used to monitor the RNAi effects. After treatment with dsRNA for 12 h, AbGPx1 expression levels and reproduction in the nematodes decreased compared with the same parameters in the control group; thus, the AbGPx1 gene is likely to be associated with the development, reproduction, and infection ability of A. besseyi. These findings may open new avenues towards nematode control.

  20. Rhabditis pellio Schneider (nematoda) from the earthworm, Aporrectodea trapezoides Duges (Annelida).

    Science.gov (United States)

    Poinar, G O; Thomas, G M

    1975-10-01

    Studies were conducted on the behavior of the nematode, Rhabditis pellio, in the earthworm, Aporrectodea trapezoides, from southern California. Juvenile and adult nematodes were found in the bladders and tubules of the metanephridia of the host. Similar nematodes that entered the coelom were encapsulated and incorporated into multiple capsules ("brown bodies"). It was demonstrated that this host response is an effective defense reaction since dead and dying nematodes, as well as living forms, were found in the capsules.

  1. Diversification of Cercopithifilaria species (Nematoda: Filarioidea in Japanese wild ruminants with description of two new species

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    Uni S.

    2002-12-01

    Full Text Available Twelve of the 17 Cervus nippon nippon deer from Kyushu Island, Japan, that we examined were infected with one or two Cercopithifilaria species. C. longa n. sp. adults were in the subcutaneous tissues of limbs and the abdomen, and C. crassa n. sp. adults were in the skin, mainly in the anterior part of the back ; the distribution of the dermal microfilariae generally matched that of the adult worms. The two new species were assigned to the group of primitive Cercopithifilaria species that parasitize ruminants (bovids and cervids, but the new species could readily be distinguished from others morphologically. C. longa was more primitive and resembled C. bulboidea, one of the five species from the serow Capricornis crispus, a Japanese member of the Caprinae, and species from Bovidae in Africa. C. crassa had a thick body and large spicules like C. rugosicauda from Capreolus capreolus in Europe, the only previously known Cercopithifilaria species from cervids, but it also had one or two hypertrophied pairs of caudal papillae, an unusual character found so far only in Japanese parasites. Among the 12 species known from ruminants, four are African, one is European and more highly evolved, and seven are Japanese, with some being primitive and some more evolved. The great diversity of Cercopithifilaria species in the two wild ruminants that live in Japan seems to have resulted from local speciation, which occurred during the Pleistocene, from a primitive form of the C. longa type derived from Eurasiatic ancestors, which has disappeared or, more probably, not yet been discovered.

  2. Falcaustra sanjuanensis sp. nov. (Nematoda: Kathlaniidae) from Odontophrynus cf. barrioi (Anura: Cycloramphidae) from Argentina.

    Science.gov (United States)

    González, Cynthya E; Sanabria, Eduardo A; Quiroga, Lorena B

    2013-03-01

    Here, we describe a new kathlaniid nematode, Falcaustra sanjuanensis sp. nov., from the large intestine of Odontophrynus cf. barrioi (Anura: Cycloramphidae), from San Juan Province, Argentina. The new species belongs to the Falcaustra group that possesses a pseudosucker. It resembles F. andrias in the distribution pattern of caudal papillae (six precloacal, four adcloacal, 12 postcloacal, one unpaired median anterior to the cloaca) but differs from F. andrias in the following characters: the longer size of males and females (11.17-13.45 mm and 10.1-15.5 mm, respectively); the longer size and form of the gubernaculum (0.17-0.23 mm, triangular form); the arrangement of postcloacal papillae (three pairs on the ventral side, two pairs on the lateral side, one pair on the subventral side) and unpaired papilla anterior to the cloaca located on the protuberance. The species description is based on light microscopy and scanning electron microscopy (SEM). Falcaustra sanjuanensis sp. nov. represents the 12th Neotropical species assigned to the genus. Also, we added a key to Neotropical species of Falcaustra.

  3. Thubunaea eleodori sp. nov. (Nematoda: Physalopteridae) from Liolaemus eleodori (Sauria: Liolaemidae) from Argentina.

    Science.gov (United States)

    Ramallo, Geraldine; Goldberg, Stephen; Bursey, Charles; Castillo, Gabriel; Acosta, Juan Carlos

    2017-01-01

    Thubunaea eleodori sp. nov. is described from the stomach of Liolaemus eleodori (Sauria: Liolaemidae) from San Guillermo National Park, Province of San Juan, Argentina. T. eleodori is most similar to those species lacking spicules, T. cnemidophorus, T. fitsimonsi, T. parkeri, T. schukurovi, and T. smogorzhewskii. T. eleodori is separated from these species based on the papilla pattern. T. eleodori has 12 pedunculate papillae and 14 sessile papillae, T. smogorzhewskii lacks pedunculate papillae, T. fitsimonsi and T. parkeri lack sessile papillae, and T. cnemidophorus has14-16 pedunculate papillae and 12 sessile papillae. T. eleodori represents the first member of the genus to be reported from Argentina.

  4. Aplikasi Cendawan Endofit terhadap Perkembangan Populasi Nematoda Radopholus similis pada Pisang Barangan

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    Lisnawita Lisnawita

    2014-09-01

    Full Text Available Radopholus similis is a major constraint to banana production in the world including Indonesia and growers have relied on nematicides to manage yield losses. The use of endophytic fungi is one method that may reduce the need for nematicides, however little is known on the effective application method of endophytic fungi to control R. similis. The aim of this research was to find out an effective application method of endophytic fungi to reduce R.similispopulation on banana. Fifteen isolates of endophytic fungi originated from banana corm and root collected from banana plantation in North Sumatera were applied to Barangan cultivar by sowing and deeping methods. The isolates was contained of 11 isolates of Fusarium sp. (2BSTMHMM, 3ASTMHP, 5ASP, 1ASU, 4BSP, 4BJP, 5BKJP, 4BSU, 1BJP, 2 BSP, and 4BKJP and 4 isolates ofTrichoderma sp. (2BSTMHP, 4BSTMHP, 2BSPH, and 2BSTMHH. All of these endophytic fungi were able to suppress the population of R. similis and they had potential to promote plant growth.

  5. Micropleura australiensis n. sp. (Nematoda: Micropleuridae) from the body cavity of Crocodylus johnsoni in Western Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Kay, W. R.; Hobbs, R. P.

    2004-01-01

    Roč. 90, č. 2 (2004), s. 322-326 ISSN 0022-3395 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematode * Philometra * marine fishes Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.439, year: 2004

  6. New observations on Micropleura australiensis (Nematoda, Dracunculoidea), a parasite of crocodiles in Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Spratt, D. M.; Kay, W. R.

    2006-01-01

    Roč. 51, č. 4 (2006), s. 273-278 ISSN 1230-2821 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Micropleura * Crocodylus * Australia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.772, year: 2006

  7. Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae in coprocultures

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    Fabio Ribeiro Braga

    Full Text Available The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722 on infective larvae (L3 of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001; group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722; group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water. The third-stage larvae (L3 were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05. The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungusD. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.

  8. Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Rudolf, Ivo; Šebesta, Oldřich; Mendel, Jan; Betášová, Lenka; Bocková, E.; Jedličková, Petra; Venclíková, Kristýna; Blažejová, Hana; Šikutová, Silvie; Hubálek, Zdeněk

    2014-01-01

    Roč. 113, č. 12 (2014), s. 4663-4667 ISSN 0932-0113 EU Projects: European Commission(XE) 261504 - EDENEXT Institutional support: RVO:68081766 Keywords : Aedes vexans * Mosquito vectors * Dirofilaria repens * Dogs * Zoonotic dirofilariosis * Setaria spp. Subject RIV: EG - Zoology Impact factor: 2.098, year: 2014

  9. Efektivitas Nematoda Patogenik Serangga (Rhabditida: Steinernema dan Heterorhabditis terhadap Penggerek Batang Padi Putih (Scirpophaga innotata

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    Chaerani Chaerani

    2006-12-01

    Full Text Available White rice stem borer (Scirpophaga innotata is a destructive insect pest which is difficult to control with various tactics. The possibility of the use of non-endemic natural enemies such as entomopathogenic nematodes (Rhabditida: Steinernema and Heterorhabditis has been investigated in green house trials. Test of 12 isolates and species of Steinernema and Heterorhabditis originated from local and exotic sources revealed that H. indicus INA H4 was the most efficacious nematode by causing 74% larval and pupal mortality within rice stems. Reduction in plant damage due to nematode application could not be demonstrated as the experiment was limited to potted plants. Subsequent test using H. indicus INA H4 showed that increasing nematode concentration from 0.5 to 2.0x10^4 IJs/ml did not significantly affect insect mortality. The survival ability of H. indicus INA H4 infective juveniles on rice plant was less than 96 hours. Improvement of nematode application strategies including repeated spray, addition of antidesiccant, increasing spray volume and application at plant damage threshold or plant critical stage are therefore necessary to increase nematode effectiveness and simultaneously to reduce plant damage in field.

  10. Efektivitas Nematoda Patogenik Serangga (Rhabditida: Steinernema dan Heterorhabditis) terhadap Penggerek Batang Padi Putih (Scirpophaga innotata)

    OpenAIRE

    Chaerani, Chaerani; Nurbaeti, Bebet

    2006-01-01

    White rice stem borer (Scirpophaga innotata) is a destructive insect pest which is difficult to control with various tactics. The possibility of the use of non-endemic natural enemies such as entomopathogenic nematodes (Rhabditida: Steinernema and Heterorhabditis) has been investigated in green house trials. Test of 12 isolates and species of Steinernema and Heterorhabditis originated from local and exotic sources revealed that H. indicus INA H4 was the most efficacious nematode by causing 74...

  11. PEMBIAKAN NEMATODA PATOGEN SERANGGA (Rhabditida: Heterorhabditis DAN Steinernema PADA MEDIA SEMI PADAT

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    . Chaerani

    2011-11-01

    Full Text Available Field application of entomopathogenic nematodes (EPN is still hampered by inefficient mass production. The aim of this study was to compare three published in vitro media (medium Wouts, Bedding and Han for mass propagation of three indigenous EPNs (Heterorhabditis indicus PLR2, H. indicus isolate 5, and Steinernema T96 and one commercial strain (S. carpocapsae #25. The media were impregnated in shredded polyurethane sponge, pre-inoculated with symbiotic bacteria of each nematode and inoculated with the respective infective juveniles (Ijs of the nematode. Nematode yields at three weeks after nematode inoculation were inconsistent accross replications and experiments and generally not significantly influenced by the kind of media tested. Average yields showed that the highest IJ productions were obtained on medium Han for  H. indicus PLR2 (0,4×105 Ijs/g medium and for S. carpocapsae #25  (2.2×105 IJs/g medium, and on  medium Wouts for H. indicus  isolate 5 (6.5×105 Ijs/g medium and Steinernema T96 (1.5×105 IJs/g medium. The Ijs’ body were significantly shorter than those of in vivo propagated, which may impair the nematode pathogenicity. Modifications of the propagation technique and media formulation are needed to improve the quantity and quality of Ijs.

  12. Natural infection of Culex theileri (Diptera: Culicidae) with Dirofilaria immitis (Nematoda: Filarioidea) on Madeira Island, Portugal.

    Science.gov (United States)

    Santa-Ana, Marta; Khadem, Manhaz; Capela, Ruben

    2006-01-01

    Field and laboratory studies were performed to verify whether Culex theileri Theobald functions as a natural vector of Dirofilaria immitis (Leidy) on Madeira Island, Portugal. CO2-baited light traps (EVS traps) were use to sample mosquitoes monthly basis between February 2002 and February 2003 in the area of Quebradas (Funchal). Three mosquito species were captured, including 58 Culex pipiens L., 790 Cx. theileri, and three Culiseta longiareolata (Macquart). Only C. theileri tested positive for D. immitis. The presence of this filarial worm was detected by direct observation, infectivity assay dissection technique, and polymerase chain reaction methods. Infected mosquitoes were recovered in October and December 2002 and January 2003. These data provide evidence that Cx. theileri could be the main vector of D. immitis in Funchal, Madeira.

  13. Two new species of Rhabdias (Nematoda: Rhabditida: Rhabdiasidae) in anuran hosts from Dehradun (Uttarakhand), India.

    Science.gov (United States)

    Rizvi, Anjum N; Bursey, Charles R; Bhutia, Pasang T

    2013-04-01

    Rhabdias himalayanus n. sp. from the lungs of Duttaphrynus himalayanus and Rhabdias dehradunensis n. sp. from the lungs of Nanorana minica from Dehradun, India are described and figured. Of the 3 previously described Indian species, Rhabdias himalayanus n. sp. is most similar to Rhabdias shortii in having a cylindrical corpus, inflated cuticle, and conical tail; it differs from R. shortii in having greater body measurements, longer esophagus, larger eggs, and a different pattern of cuticle inflation at the vulva and tail region. Rhabdias dehradunensis n. sp. is most similar to Rhabdias bulbicauda in that both possess a swollen posterior end; it differs from R. bulbicauda by having a subterminal anus, a prominent tail, and a postequatorial vulva.

  14. KEANEKARAGAMAN NEMATODA DALAM TANAH PADA BERBAGAI TIPE TATAGUNA LAHAN DI ASB-BENCHMARK AREA WAY KANAN

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    I Gede Swibawa

    2011-10-01

    Full Text Available The soil nematode diversity in several land-use types in Way Kanan ASB-Benchmark Area.  The conversion  of forest to intensive agroecosystem such as monoculture system reduces biodiversity of the plant, herbivore, and decomposer subsystems.   Those changes affected  the litter and plant root quality.  Consequently, few soil nematode species could be dominant and cause of disturbance of the stability of the below ground  community.   The increasing  populations of plant parasitic nematodes usually occur on monoculture system .   The research was conducted to study the effect of forest changes in several land use types on soil nematode diversity in Way Kanan Benchmark Area.  Soil sampling on five land use types (secondary forest,  agroforest or tree based agriculture, plantation, cassava field, and Imperata grass land was conducted in November1996 and December 1997.   Nematodes  were extracted by decantation-centrifugation with sugar method.  The soil  nematodes were grouped into order and generic level including plant parasitic and  non- parasitic. The results show that the order of  Rhabditida, Dorylaimida, and Tylenchida were found from  those five land-use types.  The total genera of  plant parasitic nematodes in Imperata grass land were the highest among the other four land use types.  The total number of  non-plant parasitic nematodes in secondary forest (28.0 individual per 300 cc of soil was higher than total number in the plantation ( 2.6 individual per 300 cc of soil, cassava field (4.0 individual per 300 cc of soil or Imperata grass land (6.6 individual per 300 cc of soil.  The total number of  plant parasitic nematodes in Imperata grass land (59.8 individual per 300 cc of soil, agroforest (59.0 individual per 300 cc of soil, secondary forest (48.2 individual per 300 cc of soil, and  plantation (17.6 individual per 300 cc of soil were not significantly different, but  total number in Imperata grass land and  agroforest  were significantly  higher than that in cassava field (11,6 individual per 300 cc of soil.

  15. Detection of Onchocerca volvulus (Nematoda: Onchocercidae) infection in vectors from Amazonian Brazil following mass Mectizan distribution.

    Science.gov (United States)

    Marchon-Silva, Verônica; Caër, Julien Charles; Post, Rory James; Maia-Herzog, Marilza; Fernandes, Octavio

    2007-05-01

    Detection of Onchocerca volvulus in Simulium populations is of primary importance in the assessment of the effectiveness of onchocerciasis control programs. In Brazil, the main focus of onchocerciasis is in the Amazon region, in a Yanomami reserve. The main onchocerciasis control strategy in Brazil is the semi-annually mass distribution of the microfilaricide ivermectin. In accordance with the control strategy for the disease, polymerase chain reaction (PCR) was applied in pools of simuliids from the area to detect the helminth infection in the vectors, as recommended by the Onchocerciasis Elimination Program for the Americas and the World Health Organization. Systematic sampling was performed monthly from September 1998 to October 1999, and a total of 4942 blackflies were collected from two sites (2576 from Balawaú and 2366 from Toototobi). The molecular methodology was found to be highly sensitive and specific for the detection of infected and/or infective blackflies in pools of 50 blackflies. The results from the material collected under field conditions showed that after the sixth cycle of distribution of ivermectin, the prevalence of infected blackflies with O. volvulus had decreased from 8.6 to 0.3% in Balawaú and from 4 to 0.1% in Toototobi.

  16. Detection of Onchocerca volvulus (Nematoda: Onchocercidae infection in vectors from Amazonian Brazil following mass Mectizan™ distribution

    Directory of Open Access Journals (Sweden)

    Verônica Marchon-Silva

    2007-03-01

    Full Text Available Detection of Onchocerca volvulus in Simulium populations is of primary importance in the assessment of the effectiveness of onchocerciasis control programs. In Brazil, the main focus of onchocerciasis is in the Amazon region, in a Yanomami reserve. The main onchocerciasis control strategy in Brazil is the semi-annually mass distribution of the microfilaricide ivermectin. In accordance with the control strategy for the disease, polymerase chain reaction (PCR was applied in pools of simuliids from the area to detect the helminth infection in the vectors, as recommended by the Onchocerciasis Elimination Program for the Americas and the World Health Organization. Systematic sampling was performed monthly from September 1998 to October 1999, and a total of 4942 blackflies were collected from two sites (2576 from Balawaú and 2366 from Toototobi. The molecular methodology was found to be highly sensitive and specific for the detection of infected and/or infective blackflies in pools of 50 blackflies. The results from the material collected under field conditions showed that after the sixth cycle of distribution of ivermectin, the prevalence of infected blackflies with O. volvulus had decreased from 8.6 to 0.3% in Balawaú and from 4 to 0.1% in Toototobi.

  17. First Record of Dicheilonema Ciconiae (Nematoda, Diplotriaenoidea From Ciconia Nigra (Aves, Ciconiidae in Ukraine

    Directory of Open Access Journals (Sweden)

    Syrota Ya. Yu.

    2016-08-01

    Full Text Available Five specimens of a parasitic nematode Dicheilonema ciconiae (Schrank, 1788 were collected from black stork, Ciconia nigra Linnaeus, in Kyiv Zoological Park. Identification of the nematodes was confirmed by morphological examination of the specimens collected. The ornamentation of the body cuticle in caudal region of males (area rugosa is first described in D. ciconiae.

  18. Komunitas Nematoda pada Tanaman Kopi (Coffea Canephora Var. Robusta Muda di Kabupaten Tanggamus Lampung

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    I GEDE SWIBAWA

    2015-09-01

    Full Text Available The Community of Nematode in The Young Coffee ( Coffea Canephora Var. Robusta Crops in Tanggamus District, Lampung. Tanggamus district is one of coffee production center in Lampung province. Since year of 2013, farmers in Tanggamus have been replaced the unproductive old coffee by coffee seed introduced from East Java. Introducing coffe seed from outside area at risk of carrying plant parasitic nematodes. The purpose of this research was to study community of nematode associated with young coffee crops in Tanggamus. Survey was conducted in coffee robusta (Coffea canephora var. robusta fields belonging to farmer on September 2014. Soil samples were collected from three sites: Margo Mulyo, Sumber Rejo and Batu Bedil. Nematodes were extracted by sieving and centrifugation with sugar solution method. The results show that were 20 genera consisted of 9 genera of plant parasitic and 11 genera of free living nematodes associated with young coffee in Tanggamus. The nematode community was dominated by Pratylenchus and Radopholus. The population of Pratylenchus and Radopholus in Sumber Rejo site were 421 and 846 individual per 300 ml of soil respectively. It was needed to indentify up to species taxonomic level for Pratylenchus and Radopholus associated with young coffee in Tanggamus.

  19. Pathology and morphometry of Hystrichis acanthocephalicus (Nematoda from Phimosus infuscatus (Pelecaniformes in southern Brazil

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    Simone Scheer

    Full Text Available Abstract Species of Hystrichis are parasite nematodes of the digestive tract of aquatic birds in South America, Europe and Asia. In Brazil, Hystrichis acanthocephalicus has been reported in Phimosus infuscatus. There are few data on the morphometry of this species and there are no reports on pathological conditions that it causes. Therefore, the purpose of this study was to report morphometric data from H. acanthocephalicus and describe the pathological effects of this parasite on the Phimosus infuscatus proventriculus. Thirty gastrointestinal tracts of P. infuscatus were examined to search for nematodes and H. acanthocephalicus occurred in 83% of hosts. Were measured the total length and body width of males and females, and of their respective cuticular spines, esophagus, spicules and eggs, and the internal and external diameter of copulatory bursa. Histopathological examination revealed parasitic structures in the proventriculus from the lumen (anterior end to the outer layers of the organ (intermediate and posterior parts, in which we observed inflammatory reaction with infiltration of heterophils, hemorrhage and hemosiderin. The results of this study of histopathology, morphometry and parasitological indices are the first ones reported to H. acanthocephalicus and should contribute to the identification and recognition in cases of outbreaks in the Neotropical region.

  20. Goezia spinulosa (Nematoda: Raphidascarididae), a pathogenic parasite of the arapaima Arapaima gigas (Osteichthyes).

    Science.gov (United States)

    Santos, Cláudia Portes; Moravec, Frantisek

    2009-03-01

    The nematode Goezia spinulosa (Diesing, 1839) (Raphidascarididae) is redescribed based on specimens found in the stomach and intestine of the naturally infected arapaima Arapaima gigas (Schinz) from the Mexiana Island, Amazon River Delta, Brazil. Light and electron microscopy examinations revealed some previously unreported or inaccurately described morphological features in the species, such as the position of the excretory pore, phasmids in the male or the number (4) of postanal papillae. The morphology of G. spinulosa is compared with that of other four congeneric species parasitizing freshwater fishes in South America. This nematode seems to be one of the most pathogenic parasites of A. gigas in the Mexiana Island, which are responsible for a high mortality of cultured arapaima fingerlings. Apparently, the source of G. spinulosa infection for arapaima fingerlings cultured in tanks was the infected plankton collected in the localities inhabited by wild arapaimas. Therefore, control measures should include the sterilisation of the plankton before its use as food for fish. A rare infection of Eustrongylides sp. larvae (Dioctophymatidae) in arapaima fingerlings was also found (new host record); the larvae were inside swellings on the body surface.

  1. Alterações teciduais em pirarucu, Arapaima gigas, infectado por Goezia spinulosa (Nematoda)

    OpenAIRE

    Menezes, Rodrigo Caldas; Santos, Sonia Maria Cursino dos; Ceccarelli, Paulo Sérgio; Tavares, Luiz Eduardo Roland; Tortelly, Rogério; Luque, José Luis

    2011-01-01

    Cinco espécimens de Arapaima gigas capturados no Rio Araguaia (Estado do Mato Grosso, Brasil) foram investigados para diagnóstico de infecção por helmintos em 2004. Numerosos espécimes adultos do nematóide rafidascarídeo Goezia spinulosa foram encontrados em úlceras do estômago circundadas por um espessamento da mucosa em todos os exemplares de A. gigas. As glândulas gástricas de todos encontravam-se necróticas e havia um acentuado e difuso infiltrado inflamatório composto por eosinófilos, qu...

  2. Integrative taxonomy of Goezia spinulosa (Nematoda: Raphidascarididae) from arapaimas in the northwestern Brazil.

    Science.gov (United States)

    Silva, Maralina Torres da; Oliveira Cavalcante, Pedro Hercílio de; Camargo, Ana Carolina Alves de; Chagas Moutinho, Vanessa Aparecida das; Santos, Everton Gustavo Nunes Dos; Santos, Cláudia Portes

    2017-08-15

    Arapaima gigas, a fish with a high market value, has been farmed in different localities within Brazil. Among its parasites, adults of Goezia spinulosa are reported to cause ulcers in the stomach and to result in the death of farmed fingerlings. Due to the veterinary importance of this nematode in cultured arapaimas, an integrative taxonomic study is proposed, combining morphological, ultrastructural and genetic profiles of this parasite. The fish were obtained from semi-intensive fish farming in Acre State, Brazil. The fish measured 7-42cm in total length and the intensity of infection was 1-60 parasites per fish. The site of infection was mainly the stomach. Morphological and ultrastructural analyses of G. spinulosa showed the importance of its spiny body in firmly attaching the worm to the gastric mucosa, resulting in lesions, ulcers and deep gastric perforations of the stomach wall. New sequences for partial 18S rDNA, ITS1, 5.8S and ITS2 rDNA, partial 28S rDNA, cox1 mtDNA and for cox2 mtDNA are presented. Phylogenetic reconstructions based on the partial 18S and 28S rDNA shows species of Goezia occur in a clade well separated from other genera in both analyses. Both the partial 18S and 28S rDNA genes represented good genetic markers for distinguishing genera of the Raphidascarididae, with exception of Hysterothylacium. This integrated taxonomic study produced a robust profile for G. spinulosa that will aid the diagnosis of both adults and larval stages from arapaimas and possible intermediate hosts. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Goezia spinulosa (Nematoda: Raphidascarididae), a pathogenic parasite of the arapaima Arapaima gigas (Osteichthyes)

    Czech Academy of Sciences Publication Activity Database

    Santos, C. P.; Moravec, František

    2009-01-01

    Roč. 56, č. 1 (2009), s. 55-63 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Goezia * Arapaima * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.266, year: 2009

  4. Tissue alterations in the pirarucu, Arapaima gigas, infected by Goezia spinulosa (Nematoda).

    Science.gov (United States)

    Menezes, Rodrigo Caldas; Dos Santos, Sonia Maria Cursino; Ceccarelli, Paulo Sérgio; Tavares, Luiz Eduardo Roland; Tortelly, Rogério; Luque, José Luis

    2011-01-01

    Five specimens of Arapaima gigas caught in the Araguaia River (State of Mato Grosso, Brazil) were investigated for helminths in 2004. Numerous adult specimens of the rhapidascarid nematode Goezia spinulosa were found in stomach ulcers in all the specimens of A. gigas and were surrounded by thickening of the mucosa. The gastric glands of all the fish were necrotic and there was a severe and diffuse inflammatory reaction composed of eosinophils (which were predominant), lymphocytes and rare macrophages in the mucosa, submucosa and muscle layer. This is the first report of tissue lesion occurrences in this host, in the presence of G. spinulosa, and it confirms the high pathogenicity of this parasite species.

  5. Nilonema gymnarchi Khalil, 1960 and N. senticosum (Baylis, 1922) (Nematoda: Dracunculoidea): Gondwana relicts?

    Science.gov (United States)

    Santos, Cláudia Portes; Gibson, David I

    2007-07-01

    Data-base searches of records of the helminth parasites of South American and African freshwater fishes revealed only two pairs of species from genera endemic to the two regions. One pair, species of the primitive amphilinid cestode genus Nesolecithus Dönges & Harder, 1966, has already been designated as likely Gondwana relicts. The second pair are the philometrid nematodes Nilonema gymnarchi Khalil, 1960 from Gymnarchus niloticus Cuvier (Gymnarchidae) in Africa and N. senticosum (Baylis, 1922) from the South American fish Arapaima gigas (Cuvier) (Arapaimidae). Both species are partly redescribed on the basis of light and scanning electron microscopical observations of the type-specimens, and their relationships are discussed. In view of the fact that both hosts are basal teleosts of the order Osteoglossiformes and they are the same two fish which harbour the amphilinid cestodes already indicated as Gondwana relics, the nematode pair is also deemed to be relictual. However, these species are suggested as being of limited potential value for calibrating a molecular clock.

  6. Three new records of Desmodorids (Nematoda, Desmodoridae) from sandy seabeds of the Canary islands

    OpenAIRE

    Riera, Rodrigo; Núñez, Jorge; Brito, María del Carmen

    2012-01-01

    In an ecological study of meiofaunal assemblages in two locations (Los Abrigos and Los Cristianos) of Tenerife (Canary Islands, NE Atlantic Ocean), several desmodorid species were found throughout the study period. Three species belonging to the family Desmodoridae were collected in intertidal and shallow subtidal sandy seabeds. These species were Desmodorella aff. tenuispiculum Allgen, 1928, Metachromadora sp. and Spirinia parasitifera Bastian, 1865. Descriptions, figures and tables with mer...

  7. Longidorus carniolensis sp. n. (Nematoda, Longidoridae from vineyard soil in Slovenia

    Directory of Open Access Journals (Sweden)

    Saša Širca

    2011-10-01

    Full Text Available A new needle nematode, Longidorus carniolensis sp. n., recovered from the soil around the roots of grapevine Vitis vinifera L. from Slovenia, is described and illustrated. Longidorus carniolensis is an amphimictic species, characterised by females with a moderately long (L=5.6–8.2 mm and plump (a=51–72.4, ave. 66.3 body, assuming a spiral to C-shape when heat relaxed. Head region continuous, anteriorly almost flat, lip region 23–25 µm wide; guiding ring situated posteriorly (42–47 µm, 43–50 µm in males, odontostyle long (ave. 146.6 (136–157 µm; pharyngeal glands with normal location, their nuclei of approximately equal size; tail bluntly conoidal to almost hemispherical. Males abundant, spicules slender and long (122–145 µm, ventromedian supplements 13–17, irregularly spaced, preceded by an adanal pair. Four juvenile stages present, the first stage juvenile with bluntly conoidal tail. Codes for identifying the new species when using the key by Chen et al. (1997 are: A 56, B 4, C 4, D 1, E 4, F 35, G 1, H 1, I 2. The new species is morphologically the most similar to L. poessneckensis Altherr, 1974, L. macrosoma Hooper, 1961, L. caespiticola Hooper, 1961, L. helveticus Lamberti et al., 2001, L. macroteromucronatus Altherr, 1974, L. pius Barsi et Lamberti, 2001, L. raskii Lamberti et Agostinelli, 1993, L. kheirii Pedram et al, 2008, L. silvae Roca, 1993, L. iuglandis Roca et al., 1985, L. vinearum Bravo et Roca, 1995 and L. major Roca et d’Erico, 1987, but differs from these species either by the body and odontostyle length, position of guide ring, head region and tail shape or the shape of the first stage juvenile tail. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other species of the genus Longidorus with known sequences. Relationships of L. carniolensis sp. n. with other Longidorus species based on analysis of this DNA fragment and morphology are discussed.

  8. The effect of different growing substrates on the development and quality of Phasmarhabditis hermaphrodita (Nematoda: Rhabditidae)

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk

    2014-01-01

    Roč. 24, č. 9 (2014), s. 1026-1038 ISSN 0958-3157 R&D Projects: GA ČR GAP504/12/2352 Institutional support: RVO:60077344 Keywords : slug parasitic nematodes * lipid reserves * progeny production Subject RIV: ED - Physiology Impact factor: 0.938, year: 2014

  9. Female morphology and systematic status of Philometroides cyprini (Nematoda: Philometridae), a parasite of carp

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Červinka, S.

    2005-01-01

    Roč. 67, 1/2 (2005), s. 105-109 ISSN 0177-5103 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometroides * Cyprinus * Czech Republic Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.361, year: 2005

  10. Redescription of Raphidascaris gigi Fujita, 1928 (Nematoda: Anisakidae), a parasite of freshwater fishes in Japan

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Nagasawa, K.

    2002-01-01

    Roč. 52, č. 3 (2002), s. 193-198 ISSN 0165-5752 R&D Projects: GA ČR GA524/00/0267 Institutional research plan: CEZ:AV0Z6022909 Keywords : nematodes * parasite of fish * Japan Subject RIV: EG - Zoology Impact factor: 0.640, year: 2002

  11. Human case of gastric infection by a fourth larval stage of Pseudoterranova decipiens (Nematoda, Anisakidae

    Directory of Open Access Journals (Sweden)

    Rubén Mercado

    1997-04-01

    Full Text Available Only three cases of human infection by anisakid nematodes have been reported in Chile since 1976. In the present case, an anisakid worm, identified as a fourth-stage Pseudoterranova decipiens larva, was removed with a gastroendoscopic biopsy clipper from the stomach of a 45 year-old man from southern Chile. The patient, who presented acute epigastric pain and a continuous sensation of having an empty stomach, reported having eaten smoked fish. The worm was fixed in 70% ethanol and cleaned in lactophenol for morphological study. The morphometric characteristics of the worm are described and drawn. Anisakid larvae in fish flesh can be killed by freezing or cooking.

  12. Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae).

    Science.gov (United States)

    Liu, Guo-Hua; Wang, Yan; Xu, Min-Jun; Zhou, Dong-Hui; Ye, Yong-Gang; Li, Jia-Yuan; Song, Hui-Qun; Lin, Rui-Qing; Zhu, Xing-Quan

    2012-12-01

    For many years, whipworms (Trichuris spp.) have been described with a relatively narrow range of both morphological and biometrical features. Moreover, there has been insufficient discrimination between congeners (or closely related species). In the present study, we determined the complete mitochondrial (mt) genomes of two whipworms Trichuris ovis and Trichuris discolor, compared them and then tested the hypothesis that T. ovis and T. discolor are distinct species by phylogenetic analyses using Bayesian inference, maximum likelihood and maximum parsimony) based on the deduced amino acid sequences of the mt protein-coding genes. The complete mt genomes of T. ovis and T. discolor were 13,946 bp and 13,904 bp in size, respectively. Both mt genomes are circular, and consist of 37 genes, including 13 genes coding for proteins, 2 genes for rRNA, and 22 genes for tRNA. The gene content and arrangement are identical to that of human and pig whipworms Trichuris trichiura and Trichuris suis. Taken together, these analyses showed genetic distinctiveness and strongly supported the recent proposal that T. ovis and T. discolor are distinct species using nuclear ribosomal DNA and a portion of the mtDNA sequence dataset. The availability of the complete mtDNA sequences of T. ovis and T. discolor provides novel genetic markers for studying the population genetics, diagnostics and molecular epidemiology of T. ovis and T. discolor. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. Spinitectus tabascoensis sp. nov. (Nematoda, Cystidicolidae) from Ictalurus furcatus (Pisces) in southeastern Mexico

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; García-Magana, L.; Salgado-Maldonado, G.

    2002-01-01

    Roč. 47, č. 3 (2002), s. 224-227 ISSN 1230-2821 R&D Projects: GA AV ČR IAA6022901 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematode * Spinitectus * fish Subject RIV: EG - Zoology Impact factor: 0.732, year: 2002

  14. Angiostrongylus costaricensis n. sp. (Nematoda: Metastrongyloidea, a new lungworm occurring in man in Costa Rica

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    Pedro Morera

    2002-06-01

    Full Text Available Angiostrongylus costaricensis n. sp. is described from Costa Rica where it produces lesions in the abdominal cavity of man. It can be distinguished from other species of the genus on the basis of its size, the length of the spicules, the position of the vulva and the morphology and position of the bursal rays. The parasite localizes in the small mesenteric arteries, especially in the ileocecal region, where it produces arteritis and thrombosis. Eggs in various stages of embryonation were found scattered in the tissues of the intestinal wall and regional lymph nodes, eliciting a granulomatous inflammatory reaction with in. tense eosinophilic infiltrationLos autores hacen la descripción de Angiostrongylus costaricensis n. sp., un nuevo metastrongilideo encontrado en Costa Rica, que produce lesiones en el hombre. Esta nueva especie se puede distinguir de las otras Catorce descritas hasta ahora, en base a su tamaño, la longitud de las espículas, la posición de la vulva y la morfología y disposición de los rayos bursales. A. costaricensis es la segunda especie del género de importancia en parasitología humana. La otra especie patógena para el hombre, A. cantonensis, es responsable de una forma de meningoencefalitis eosinofílica. Los parásitos adultos, machos y hembras, se localizan en las arterias del mesenterio y de la pared intestinal, especialmente de la región ileocecal en donde provocan fenómenos inflamatorios y trombóticos que llevan a grados diversos de necrosis. La presencia de huevos en varios estados de embrionación, en el tejido de la pared intestinal y de los ganglios linfáticos regionales, produce una reacción inflamatoria granulomatosa con intensa infiltración eosinofilica. La pared intestinal se presenta engrosada, llegándose a producir cuadros de suboclusión u oclusión que obligan a intervenciones quirúrgicas de emergencia. La presencia 4e huevos en los tejidos humanos sugiere una mejor adaptación de A. costaricensis en el hombre, en comparación con A. cantonensis, que no llega a alcanzar el estado adulto en los tejidos encefálicos humanos. Sin embargo en los casos en que hemos encontrado larvas completamente formadas, ellas siempre están encerradas en la cáscara del huevo, lo que sugiere la imposibilidad de que las mismas lleguen a la luz intestinal y regresen al suelo para completar su ciclo de vida

  15. Reevaluation of Physaloptera bispiculata (Nematoda: Spiruroidaea) by light and scanning electron microscopy.

    Science.gov (United States)

    Mafra, A C; Lanfredi, R M

    1998-06-01

    This study was undertaken to clarify several aspects of morphological and taxonomic characters of Physaloptera bispiculata Vaz and Pereira, 1935, a parasite of the water rat, Nectomys squamipes. The cephalic structures (including lips, papillae, teeth, amphids, and porous areas) and details of the posterior end of male and female adult worms were examined by scanning electron microscopy, leading to the addition of new taxonomic characters for this species. We consider P. bispiculata a valid species, based on a comparative analysis of the specific characters for P. bispiculata and P. getula Seurat, 1917, including the morphology and morphometry of body structures as well as number and disposition of caudal papillae of the males.

  16. Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for oxyuroid species (Nematoda).

    Science.gov (United States)

    Jobet, E; Bougnoux, M E; Morand, S; Rivault, C; Cloarec, A; Hugot, J P

    1998-03-01

    Random amplified DNA markers (RAPD; Williams et al., 1990) were used to obtained specific RAPD fragments characterising different species of oxyuroids. We tested six species of worms parasitizing vertebrates or invertebrates: Passalurus ambiguus Rudolphi, 1819, parasite of Leporids; Syphacia obvelata (Rudolphi, 1802) Seurat, 1916, a parasite of rodents; Blatticola blattae (Graeffe, 1860) Chitwood, 1932 parasite of the cockroach Blattella germanica; Hammerschmidtiella diesingi (Hammerschmidt, 1838) Chitwood, 1932 and Thelastoma bulhoesi (Magalhaes, 1990) Travassos, 1929, parasites of the cockroach Periplaneta americana, and an undescribed parasite species of a passalid insect from New Caledonia. Among 15 oligonucleotides tested, nine produced several specific bands allowing the interspecific discrimination.

  17. A redescription of Protospirura muricola Gedoelst, 1916 (Nematoda: Spiruridae), a parasite of murid rodents.

    Science.gov (United States)

    Smales, L R; Harris, P D; Behnke, J M

    2009-01-01

    The spirurid nematode Protospirura muricola Gedoelst, 1916 is redescribed from Acomys dimidiatus (Desmarest) from the St Katherine Protectorate, Sinai, Egypt. Egyptian material closely resembled specimens of P. muricola from African mammals re-examined in this study, as well as conforming to published reports of this species. P. muricola with two denticles on each lateral lobe of the pseudolabia and six pairs of postanal papillae is closest to P. pseudomuris Yokohata & Abe, 1989, but can be readily distinguished in having the right spicule shorter than the left. The significance of the characteristics of the head and mouth, and of the male spicules, in characterising Protospirura Seurat, 1914 is evaluated. P. muricola, an African parasite of rodents, appears to have spread globally with synanthropic rat final hosts and possibly with the cosmopolitan dermapteran intermediate host Leucophaea maderae (Fabr.).

  18. Parasitization of a huntsman spider (Arachnida: Araneae: Sparassidae: Heteropoda venatoria by a mermithid nematode (Nematoda: Mermithidae

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    Sachin P. Ranade

    2016-11-01

    Full Text Available The emergence of a mermithid worm from a huntsman spider Heteropoda venatoria was witnessed at Buxa Tiger Reserve, West Bengal.  It appears to be a first record of the spider family Sparassidae serving as a host for a member of the family Mermithidae. 

  19. Mexiconema africanum sp. n. (Nematoda: Daniconematidae) from the catfish Auchenoglanis occidentalis from Lake Turkana, Kenya

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Jirků, Miloslav; Charo-Karisa, H.; Mašová, Š.

    2009-01-01

    Roč. 105, č. 4 (2009), s. 1047-1052 ISSN 0932-0113 R&D Projects: GA MŠk LC522; GA AV ČR KJB600960813 Institutional research plan: CEZ:AV0Z60220518 Keywords : Mexiconema * Auchenoglanis * Kenya Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.721, year: 2009

  20. The influence of pesticides on the viability and infectivity of entomopathogenic nematodes (Nematoda: Steinernematidae)

    Czech Academy of Sciences Publication Activity Database

    Nermuť, Jiří; Mráček, Zdeněk

    2010-01-01

    Roč. 18, č. 2 (2010), s. 141-148 ISSN 0869-6918 Institutional research plan: CEZ:AV0Z50070508 Keywords : Steinernema feltiae * Steinernema arenarium * Steinernema kraussei Subject RIV: ED - Physiology Impact factor: 0.500, year: 2010

  1. New observations on Mexiconema cichlasomae (Nematoda: Dracunculoidea) from fishes in Mexico.

    Science.gov (United States)

    Moravec, F; Jiménez-García, M I; Salgado-Maldonado, G

    1998-09-01

    The dracunculoid nematode Mexiconema cichlasomae Moravec, Vidal et Salgado Maldonado, 1992, originally described from the abdominal cavity and viscera of Cichlasoma spp. from Mexico, was recorded from the abdominal cavity of the poeciliid Xiphophorus helleri Heckel in Lake Catemaco and its small tributary Arroyo Agrio, Veracruz and from the intestine of the nurse shark Ginglystoma cirratum (Bonnaterre) off the southern coast of the Gulf of Mexico in Campeche; both these findings represent new host records. The nematode specimens from these hosts are briefly described and illustrated. Whereas X. helleri evidently served as the true definitive host of this parasite, G. cirratum probably acquired Mexiconema infection accidentally while feeding on fish definitive hosts in the brackish or salt-water environment. The ability of M. cichlasomae to utilize fishes of different orders (Perciformes and Cyprinodontiformes) as definitive hosts is rather exceptional among dracunculoid nematodes.

  2. Rhabdochona spp. (Nematoda: Rhabdochonidae) from fishes in the Central African Republic, including three new species

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Jirků, Miloslav

    2014-01-01

    Roč. 61, č. 2 (2014), s. 157-172 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Globochona * freshwater fish * Barbus * Epiplatys * Marcusenius * Phenacogrammus * Raiamas * Congo River basin Subject RIV: EG - Zoology Impact factor: 1.147, year: 2014

  3. Two Procamallanus (Spirocamallanus) species (Nematoda: Camallanidae) from freshwater fishes in the Lower Congo River

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Jirků, Miloslav

    2015-01-01

    Roč. 60, č. 2 (2015), s. 226-233 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * new species * redescription * Synodontis * Parachanna * Teleostei * Africa Subject RIV: EG - Zoology Impact factor: 1.293, year: 2015

  4. Changes in the viability of Strongyloides ransomi larvae (Nematoda, Rhabditida under the influence of synthetic flavourings

    Directory of Open Access Journals (Sweden)

    A. A. Boyko

    2017-01-01

    Full Text Available One of the most common nematodes of pigs globally is Strongyloides ransomi Schwartz and Alicata 1930. It usually causes aggravation of physiological indicators of its hosts and damage to their immune system. Also it is a good modelling object for the evaluation of the antiparasitic activity of new antihelminthic drugs. We conducted laboratory experiments to assess the effect of flavouring additives with flower odour (benzaldehyde, citral, D-limonene and β-ionone upon the viability of S. ransomi larvae. The mortality rate was calculated for 24 hours exposure at four concentrations of each substance (10, 1, 0.1 и 0.01 g/l with eight replications. The lowest LD50 values were obtained for citral (97 mg/l and benzaldehyde (142 mg/l. These substances are recommended for further evaluation of their antihelminthic effect in experiments using laboratory animals. Unlike other substances, the effect of β-ionone and D-limonene even at a concentration of 10 g/l after 24 hours caused the death of <50% of S. ransomi larvae. The study of flavouring additives with flowery odour, which are permitted to be used in food for humans and also to be used in cosmetics, is a promising field for research aimed at the development of new antiparasitic drugs.

  5. Parasites diversity in carnivorous animals in the territory of Dnipropetrovsk

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    О. О. Boyko

    2011-07-01

    Full Text Available In Dnipropetrovsk sity (Ukraine, Dnipropetrovsk region in carnivorous animals 10 species of parasites (helminths and coccidia were found: Uncinaria sp., Ancylostoma sp., Dictyocaulus immitis (Nematoda, Strongylata, Strongyloides stercoralis (Nematoda, Rhabditata, Spirocerca lupi (Nematoda, Spirurata, Toxocara canis (Nematoda, Ascaridata, Trichuris vulpis (Nematoda, Trichurata, Dipylidium caninum (Cestoda, Hymenolepidata, Cystoisospora sp. and Toxoplasma gondii (Sporozoa, Coccidia. In soil S. stercoralisand Uncinaria sp. weredominanted. In most carnivorous animals registered in L. Globa park and T. Shevchenko park the S. stercoralisand Uncinaria sp., Cystoisosporasp. and T. gondii were found.

  6. Seasonal abundance of equine strongyles (Nematoda: Strongylidae in the State of Rio de Janeiro, Brazil Abundância sazonal de estrongilídeos de eqüinos (Nematoda: Strongylidae no Estado do Rio de Janeiro

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    I.V.F. Martins

    2005-02-01

    Full Text Available The seasonal abundance of lumenal and larval forms of equine strongyles in the State of Rio de Janeiro, Brazil was evaluated. Thirty horses referred to the Federal Rural University of Rio de Janeiro were necropsied and their gastrointestinal tracts were separated into stomach, small intestine, cecum, ventral colon, dorsal colon and rectum. Two aliquots of 5% of the content were collected and examined for the presence of small strongyles and the rest of the content was examined for the presence of large strongyles. The mesenteric artery, liver, pancreas and peritoneum were examined for the presence of strongyle larvae. The mucosa of intestine segments was cut into parts of equal length, and each one was examined by the mural transillumination technique. Higher abundances of larvae of Strongylus equinus (PEstudou-se a ocorrência sazonal de formas larvares e adultas de estrongilídeos nas mucosas do estômago, intestino delgado, ceco, colon ventral, colon dorsal e reto de 30 eqüídeos no Estado do Rio de Janeiro. Os grandes estrongilídeos foram encontrados em todas as mucosas e duas alíquotas de 5% do conteúdo foram retiradas para a procura de pequenos estrongilídeos. Artéria mesentérica, fígado, pâncreas e peritônio foram também examinados para larvas de estrongilídeos. As mucosas foram examinadas pela técnica de transiluminação. Larvas de Strongylus equinus (P<0,10 e adultos de Strongylus vulgaris (P<0,05 foram mais abundantes na estação seca. Larvas escistadas de ciatostomíneos o foram na estação chuvosa (P<0,02.

  7. Experimental infection in Notodiaptomus sp. (Crustacea: Calanoida with larvae of Camallanus sp. (Nematoda: Camallanidae Infecção experimental em Notodiaptomus sp. (Crustacea: Calanoida com larvas de Camallanus sp. (Nematoda: Camallanidae

    Directory of Open Access Journals (Sweden)

    M.L. Martins

    2007-04-01

    Full Text Available This trial registered the experimental infection viability with nematode larvae Camallanus sp. in Notodiaptomus sp., a crustacean, which can be an intermediate host. Adult females of nematode were dissected from the intestines of Xiphophorus maculatus (Osteichthyes: Poeciliidae, at a fish farm in the State of São Paulo. Females were slightly compressed for larvae release, collected with Pasteur pipette and separated on Petri dishes with 9ml filtered water at 28.1ºC, from zooplankton culture. Treatments consisted of Petri dishes with 60 and 105 copepods, in which 120, 150 and 210 larvae of nematode were added in four replications. Twenty-four and 36h after exposition to the larvae, the copepods were fixed in 70% alcohol to record the amount of fixed larvae. Twenty four hours after exposition, 60 copepods group with 120 larvae showed significantly higher prevalence (46.5% when compared to 105 copepods and 120 larvae (33.2%. Thus, these answers suggested that 120 larvae were enough for a successful infectivity. Experimental infection was available and so, it was used as a pattern to life cycle studies of camallanid nematodes and hosts susceptibility tests.A viabilidade da infecção experimental com larvas do nematóide Camallanus sp. em Notodiaptomus sp., crustáceo com potencial para hospedeiro intermediário foi avaliada. Fêmeas adultas do nematóide foram extraídas de Xiphophorus maculatus (Osteichthyes: Poeciliidae, provenientes de piscicultura de peixes ornamentais no estado de São Paulo. As fêmeas foram ligeiramente pressionadas para liberar as larvas, coletadas com pipeta Pasteur e separadas em placas de Petri contendo 9ml de água filtrada a 28,1ºC do próprio cultivo de zooplâncton. Os tratamentos consistiram de placas contendo 60 e 105 copépodes onde se adicionou 120, 150 e 210 larvas de nematóides em quatro repetições. Nos tempos de 24 e 36h após a exposição às larvas, os copépodes foram fixados em álcool 70% para quantificação de larvas. Após 24h de exposição, o grupo com 60 copépodes na presença de 120 larvas apresentou maior prevalência (46,5% do que 105 copépodes com 120 larvas (33,2%. Sugere-se que 120 larvas foram suficientes para o sucesso da infecção. A infecção experimental mostrou-se viável, servindo de modelo para o estudo do ciclo de vida de camalanídeos e testes de susceptibilidade de hospedeiros.

  8. Una especie nueva de Cheiropteronema (Nematoda, Molineidae en Artibeus planirostris (Chiroptera, Phyllostomidae en la Argentina A new species of Cheiropteronema (Nematoda, Molineidae in Artibeus planirostris (Chiroptera, Phyllostomidae from Argentina

    Directory of Open Access Journals (Sweden)

    Mirna C Oviedo

    2010-09-01

    Full Text Available Se describe a Cheiropteronema striatum sp. nov. parásita del murciélago Artibeus planirostris (Spix, 1823. Se caracteriza por presentar vesícula cefálica estriada, superficie de los huevos con puntuaciones, espículas iguales, nacimiento simétrico del ala espicular, particular disposición y número de crestas del synlophe y por carecer de diente esofágico. Es la segunda especie del género y la primera que se registra en quirópteros frugívoros de la Argentina.Cheiropteronema striatum sp. nov. is a parasite of the bat Artibeus planirostris (Spix, 1823. This species is characterized by cephalic vesicle striate, thick egg shell punctate, spicules equal, symmetrical development of spicule wings, particular arrangement and number the ridges of synlophe, and lack of esophageal tooth. Cheiropteronema striatum sp. nov. is the second species described for the genus and the first recorded in frugivorous bats of Argentina.

  9. Dichelyne (Dichelyne) spinigerus sp. nov. (Nematoda: Cucullanidae) from the marine fish Otolithes ruber (Sciaenidae) off Iran and first description of the male of Philometra otolithi Moravec et Manoharan, 2013 (Nematoda: Philometridae)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Khosheghbal, M.; Pazooki, J.

    2014-01-01

    Roč. 59, č. 2 (2014), s. 229-237 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Dichelyne * Philometra * marine fish * Otolithes * Iran Subject RIV: EG - Zoology Impact factor: 0.905, year: 2014

  10. Nematodes from the caecum and colon of Pogonomys (Muridae: Anisomyini) from Papua New Guinea with the descriptions of a new genus of Oxyuridae (Nematoda: Oxyurida) and a new species of Trichuridae (Nematoda: Enoplida).

    Science.gov (United States)

    Smales, L R

    2013-01-10

    Nematodes, comprising 2 species, a new genus from the family Syphaciidae and a new species from the family Trichuridae were collected from the lower digestive tracts of 4 species of Pogonomys; P. championi, Flannery (12 individuals), P. loriae, Thomas (14 individuals), P. macrourus, (Milne Edwards) (19 individuals) and P. sylvestris, Thomas (27 individuals) from Papua, Indonesia and Papua New Guinea. Pogonomicola rugala n. gen., n. sp. differs from all other genera in the Sypaciidae in having cervical alae with numerous folds and a single weakly defined mamelon. Trichuris germani n. sp. differs from all congeners, including the cosmopolitan T. muris, the only other trichurid reported from the region, by the lengths of the spicules and vagina, the ratio of anterior to posterior body length and the number of convolutions of the testis. The genus Pogonomys, with four species from four nematode families had a relatively rich helminth fauna in the lower digestive tract compared to other ansomyins studied. The Oxyuridae, with three genera comprising 5 species was the dominant group found in the lower digestive tract of the Anisomyini, indicating the possibility that the isolation of the old endemic rodents in New Guinea has been associated with a period of coevolution between anisomyin hosts and their syphaciine parasites.

  11. Proteolytic action of the crude extract of Duddingtonia flagrans on cyathostomins (Nematoda: Cyathostominae in coprocultures Ação proteolítica do extrato bruto de Duddingtonia flagrans sobre ciatostomíneos (Nematoda: Cyathostominae em coproculturas

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    Fabio Ribeiro Braga

    2013-03-01

    Full Text Available The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722 on infective larvae (L3 of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001; group 2: 10 mL of crude extract of AC001 with 10 mM of Ca2+; group 3: 10 mL of crude extract of D. flagrans (CG722; group 4: 10 mL of crude extract of CG722 with 10 mM of Ca2+; and group 5: control group (distilled water. The third-stage larvae (L3 were obtained after eight days. The crude extract of D. flagrans was effective in reducing the number of L3, with the following percentage reductions: group 1, 49.5%; group 2, 52.5%; group 3, 36.8%; and group 4, 57.7%; in relation to the control group (p > 0.05. The proteolytic activity of the crude extract was confirmed through the zymogram. The results from this study confirmed that the crude extract of the fungus D. flagrans could be used for controlling cyathostomin L3, and suggested that at least one protease of approximately 38 kDa was present.O objetivo deste trabalho foi estudar a ação do extrato bruto de Duddingtonia flagrans (isolados AC001 e CG722 sobre larvas infectantes (L3 de ciatostomíneos em coproculturas e confirmar a sua atividade proteolítica por meio de um zimograma. Foram formados os seguintes grupos em coproculturas: grupo 1: 10 mL de extrato bruto de D. flagrans (AC001; grupo 2: 10 mL de extrato bruto de AC001 com íons Ca2+ 10 Mm; grupo 3: 10 mL de extrato bruto de D. flagrans (CG722; grupo 4: 10 mL de extrato bruto de CG722 com íons Ca2+ 10 Mm; e grupo 5 como controle (água destilada, obtendo-se as L3 ao final de 8 dias. O extrato bruto de D. flagrans foi eficiente na redução do número de L3 com os seguintes percentuais de redução: grupo 1 (49,5%; grupo 2 (52,5%; grupo 3 (36,8% e grupo 4 (57,7% em relação ao grupo controle (p > 0,05. Confirmou-se a atividade proteolítica por meio do zimograma. Os resultados do presente trabalho confirmam a utilização do extrato bruto do fungo D. flagrans no controle de L3 de ciatostomíneos e sugere a presença de pelo menos uma protease de aproximadamente 38 kDa.

  12. Echinuria uncinata (Rudolphi (Nematoda, Acuariidae in Netta peposaca (Vieillot (Aves, Anatidae in South America Echinuria uncinata (Rudolphi (Nematoda, Acuariidae em Netta peposaca (Vieillot (Aves, Anatidae na América do Sul

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    Eliane F. da Silveira

    2006-06-01

    Full Text Available This is the first report of a species of Echinuria Soloviev, 1912, on anatid hosts in South America, causing granulomas. It is also the first detailed description and record of a species of Echinuria, for South America in the Rosy-billed Pochard, Netta peposaca (Vieillot, 1816. Fifty-two rosy-billed pochards were examined for helminths in the Municipalities of Santa Vitória do Palmar (locality of Fazenda Sossego and (locality of Ponta da Antena, State of Rio Grande do Sul, Brazil, and Alvear, Province of Corrientes, northern Argentina. Samples were obtained from 2003 to 2004. After the catch each bird was immediately frozen with dry ice. Prior to necropsy birds were sexed, weighted, measured, and the state of maturity (juvenile or adult determined, based on the presence or absence of a bursa of Fabricius. The granulomas with the nematodes were found in the proximal esophagus at the junction with the proventriculus. Two birds had one (fistulated and two granulomas, respectively. One of the birds was from the wintering grounds along the coastal region in the State of Rio Grande do Sul, southern Brazil. The other, from one of the breeding grounds in northern Argentina, was captured before the trip to the wintering grounds along the migratory flyway. Prevalence was 3.8% while the mean intensity of infection was 7.2. Morphometry of males and females and the comparison with previous descriptions and illustrations allowed the identification of the present specimens as E. uncinata.Este é o primeiro registro de uma espécie do gênero Echinuria Soloviev, 1912, em hospedeiros anatídeos na América do Sul, causando granulomas. É, também, a primeira descrição detalhada e o primeiro registro de Echinuria uncinata (Rudolphi, 1819 Soloviev, 1912, para a América do Sul, e o primeiro registro de um nematóide acuarióideo em Netta peposaca (Vieillot, 1816. Cinqüenta e dois marrecões foram examinados para helmintos nas localidades de Fazenda Sossego e Ponta da Antena, Município de Santa Vitória do Palmar, Estado do Rio Grande do Sul, região Sul do Brasil e Alvear, Província de Corrientes, região Norte da Argentina. As amostras foram obtidas no período de 2003 a 2004. Imediatamente após o abate, cada ave foi congelada em gelo seco. As aves foram separadas por sexo, pesadas e medidas. O estado de maturidade de cada ave foi avaliado (juvenil ou adulto com base na presença ou ausência da bolsa de Fabricius. Os granulomas, contendo os nematóides, estavam situados na base do esôfago quase junto à junção com o proventrículo. Duas aves estavam positivas, com um (fistulado e dois granulomas, respectivamente. Uma das aves era proveniente do Brasil (pólo de invernia na planície costeira do Estado do Rio Grande do Sul. A outra da região Norte da Argentina, capturada em um dos pólos de nidificação, antes de empreender sua viagem em direção à costa brasileira ao longo de sua rota de migração. A prevalência da infecção foi de 3,8% e a intensidade média de infecção de 7,2. A morfometria de machos e de fêmeas permitiu a identificação dos espécimes como E. uncinata.

  13. New data on the morphology of Dichelyne hartwichi (Nematoda, Cucullanidae), a parasite of freshwater tetraodontid fishes (Tetraodon spp.) in Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Fiala, Ivan; Dyková, Iva

    2011-01-01

    Roč. 56, č. 4 (2011), s. 433-437 ISSN 1230-2821 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Parasitic nematode * Dichelyne * freshwater fish * Tetraodon * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.789, year: 2011

  14. Raphidascaris (Ichthyascaris) arii sp n. (Nematoda: Anisakidae), a new ascaridoid nematode from marine catfishes in the Gulf of Thailand

    Czech Academy of Sciences Publication Activity Database

    Yooyen, T.; Moravec, František; Wongsawad, C.

    2011-01-01

    Roč. 48, č. 4 (2011), 262-267 ISSN 0440-6605 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : parasitic nematode * Raphidascaris * Ichthyascaris * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.773, year: 2011

  15. A new species of Rhabdochona Railliet, 1916 (Nematoda: Rhabdochonidae) from cyprinid fishes in the Western Ghats Region, India

    Czech Academy of Sciences Publication Activity Database

    González-Solís, David; Chavan, S. P.; Kannewad, P.; Gyananath, G.

    2014-01-01

    Roč. 87, č. 3 (2014), s. 273-281 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : fresh water fishes * morphology * Thailand Subject RIV: EA - Cell Biology Impact factor: 1.336, year: 2014

  16. Two new sibling species of Procamallanus (Spirocamallanus) (Nematoda: Camallanidae) from marine fishes in the Gulf of Thailand

    Czech Academy of Sciences Publication Activity Database

    Yooyen, T.; Moravec, František; Wongsawad, C.

    2011-01-01

    Roč. 97, č. 5 (2011), 931-938 ISSN 0022-3395 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Procamallanus * Perciformes * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.405, year: 2011

  17. Rhabdochona (Rhabdochona) hypsibarbi n. sp. (Nematoda: Rhabdochonidae) from the Freshwater Cyprinid Fish Hypsibarbus wetmorei (Smith) in Northeast Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Pachanawan, A.; Kamchoo, K.

    2013-01-01

    Roč. 99, č. 2 (2013), s. 297-302 ISSN 0022-3395 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Parasitic nematode * Rhabdochona * Thailand Subject RIV: EA - Cell Biology Impact factor: 1.258, year: 2013

  18. Two new species of Cucullanus Mu¨ ller, 1777 (Nematoda: Cucullanidae) from marine fishes off Thailand

    Czech Academy of Sciences Publication Activity Database

    Yooyen, T.; Moravec, František; Wongsawad, C.

    2011-01-01

    Roč. 78, č. 2 (2011), s. 139-149 ISSN 0165-5752 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cucullanus * marine fish * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.250, year: 2011

  19. Description of Rhabdochona (Globochona) rasborae sp. n. (Nematoda: Rhabdochonidae) from the freshwater cyprinid fish Rasbora paviana Tirant in southern Thailand

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Kamchoo, K.

    2012-01-01

    Roč. 59, č. 3 (2012), s. 209-215 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Rhabdochona * Globochona * freshwater fish * sidestripe rasbora * Cyprinidae * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/detail.php?id=22068

  20. The morphology and systematics of Rhabdochona paski Baylis, 1928 (Nematoda: Rhabdochonidae), a widespread parasite of freshwater fishes in Africa

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Charo-Karisa, H.; Jirků, Miloslav

    2013-01-01

    Roč. 85, č. 1 (2013), s. 55-63 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112; GA AV ČR KJB600960813 Institutional support: RVO:60077344 Keywords : Parasitic nematode * Rhabdochona * Africa Subject RIV: EA - Cell Biology Impact factor: 1.035, year: 2013

  1. Pathomorphological changes in aterina Atherina boyeri liver under invasion of Contracaecum rudolphii l. Hartwich, 1964 (Nematoda: Anisakidae

    Directory of Open Access Journals (Sweden)

    Popyuk M. P.

    2017-06-01

    Full Text Available Parasitological examination of Black Sea aterina (Atherina boyeri has been held in two districts of the Black Sea, Crimean seashore. Fish liver infected by larvae of Contracaecum rudolphii l. has been studied with classical histology and microscopy, the comparative analysis of the obtained data has been carried out with materials of invasion free individuals. It has been observed that pathomorphological changes occur in the aterine liver under C. rudolphii l. parasitizing. Particularly, the parenchyma destruction, tissue dystrophy, microcirculation disorders have been registered. It has been found that the most pronounced changes are recorded in the liver because of severe infestation of nematodes from the aterina (A. boyeri: the organ is significantly increased, the edges are rounded, tuberous surface, coloring is light, uneven; the cut liver obtains visible mosaic pattern due to moves done by the larvae of the nematode C. rudolphii l. in the liver parenchyma. Microscopy of histological sections in cases of invasions in the liver parenchyma shows signs of acute congestive hyperemia: central veins and capillaries lobules located closer to them are full of blood and expanded. Hepatocytes in the center of the lobules because of compression of dilated capillaries are subjected to atrophy. In bile ducts acute catarrh accompanied by focal hyperplasia of the epithelial cells is observed; in the aperture of the bile ducts accumulation of mucus with a mixture of desquamated epithelial cells and macrophages is seen.

  2. First report of Litomosa spp. (Nematoda: Filarioidea) from Malagasy bats; review of the genus and relationships between species.

    Science.gov (United States)

    Martin, C; Bain, O; Jouvenet, N; Raharimanga, V; Robert, V; Rousset, D

    2006-03-01

    The presence of the filarial genus Litomosa in Malagasy bats is demonstrated by the finding of L. goodmani n. sp. from Miniopterus gleni and Litomosa sp. (male unknown) from M. manavi, both in the Special Reserve of Ankarana. These materials are compared to the 22 Litomosa species, including two Indian species originally placed in the genus Litomosoides, L. fotedari (Gupta and Trivedi, 1989) n. comb. and L. tewarii (Gupta and Trivedi, 1989) n. comb., and the new taxon L. seurati n. sp. (= L. beaucournui Bain, 1966 pro parte), type-host Rhinolophus ferrum-equinum, Algeria, distinguished by the narrow area rugosa and the female caudal extremity with two conspicuous points, instead of several small ones. The Malagasy material belongs to a group of species close to the type, L. filaria, which have a male area rugosa composed of cuticular bosses and microfilariae folded within the sheath, and which are parasitic in Vespertilionidae, Hipposideridae and Rhinolophidae from Africa and Europe. The two Malagasy species resemble L. seurati n. sp., L. beshkovi Jancev, 1971, L. chiropterum Ortlepp, 1932, L. adami Petit, 1980 and L. ottavianii Lagrange et Bettini, 1948, with the enlarged third segment of the buccal capsule. L. goodmani n. sp. is distinct with its small size and female caudal extremity with a single point, which is suppressed in old mature worms; the females of Litomosa sp. have two conical points. Relationships among Litomosa species appear to be dependent upon both the chiropteran host groups and the geographical region.

  3. Phylogenetic Relationships of Cucullanidae (Nematoda), with Observations on Seuratoidea and the Monophyly of Cucullanus, Dichelyne and Truttaedacnitis.

    Science.gov (United States)

    Choudhury, Anindo; Nadler, Steven A

    2016-02-01

    The phylogenetic relationships of Cucullanidae were explored using near-complete sequences of the 18S rDNA (rRNA gene). Sequences (1,750-1,760 bp) were obtained from 7 species of Cucullanidae belonging to 3 genera, Cucullanus (2 spp.), Dichelyne (2 spp.), Truttaedacnitis (3 spp.), and 1 species of Quimperiidae ( Paraseuratum sp.). These sequences were aligned with those of 128 other nematode species available in GenBank, including 3 other cucullanids (Dichelyne mexicanus, Cucullanus robustus, and Cucullanus baylisi) and 2 non-cucullanid seuratoids (Paraquimperia africana, and Linstowinema sp.). Bayesian (BPP) and maximum likelihood (ML) analyses of 2 different datasets strongly supported a monophyletic Cucullanidae. Bayesian analysis placed this family as the sister group to a clade containing species of Diplogasterida, Strongylida, Rhabditida, and Tylenchida with very strong support. Neither BPP nor ML analyses recovered a close relationship of Cucullanidae to Ascaridida. None of the 3 non-cucullanid seuratoid species were sister to Cucullanidae, nor did they form a monophyletic group of their own, which questions the monophyly of Seuratoidea and the relationships among species within this superfamily. The 3 genera of cucullanids were also not monophyletic, although morphologically similar species such as the 2 species of Cucullanus from Neotropical catfishes and 2 species of Dichelyne from Nearctic ictalurid catfishes were sister taxa with strong support. The results were ambiguous with respect to the relationship of 2 Truttaedacnitis spp. in Nearctic freshwater fishes but do not support Truttaedacnitis heterodonti, a parasite of heterodontid sharks, as belonging to this genus. The study shows that all aspects of the conventional classification of Seuratoidea and its taxa should be scrutinized by even more extensive sampling across hosts and habitats.

  4. Scanning electron microscopy of Turgida turgida (Nematoda: Spiruroidea), parasite of the Virginia opossum, Didelphis virginiana, from southern California.

    Science.gov (United States)

    Matey, V E; Kuperman, B I; Kinsella, J M

    2001-10-01

    Taxonomic characters for identification of Turgida turgida Rudolphi, 1819, a parasitic nematode of the Virginia opossum Didelphis virginiana, were studied by scanning electron microscopy. The distinguishing feature of the cephalic end is the presence of numerous denticles, structures associated with the internal tooth, and 2 spongelike areas on the inner side of each pseudolabia. The posterior end of male T. turgida differs from that in other species of Physalopteridae by the number of caudal papillae (22), truncated postcloacal papilla, and patterns of ventrocaudal ornamentation.

  5. Histopathological changes in the liver and stomach of Didelphis virginiana (Didelphimorphia: Didelphidae) during natural infection with Gnathostoma turgidum (Nematoda: Gnathostomidae).

    Science.gov (United States)

    Torres-Montoya, E H; Zazueta-Moreno, J M; Osuna-Martínez, L U; Castillo-Ureta, H; Silva-Hidalgo, G; López-Moreno, H S; Osuna-Ramírez, I; Noguera-Corona, E; Rendón-Maldonado, J G

    2017-11-06

    Gnathostoma turgidum is a nematode parasite that exploits the stomach of Virginian opossums, Didelphis virginiana, in Latin America. The opossum is the definitive host of G. turgidum in the wild. Intrahepatic growth and maturation of the parasite, subsequent migration to the stomach and spontaneous expulsion are common. However, the histopathological lesions caused by G. turgidum are poorly described. A better understanding of the life cycle of this parasite and the pathological changes in natural host-parasite interactions could help to clarify the progression of human infections caused by Gnathostoma binucleatum. The aim of this work was to study morphological changes in the liver and stomach of D. virginiana during natural infection and adult worm expulsion. Three opossums naturally infected with G. turgidum were captured from an endemic area of gnathostomosis. Three uninfected opossums captured from a non-endemic area were used as controls. The opossums were sacrificed at different stages of infection (March, May and December), and a histopathological study of their livers and stomachs was conducted. Injuries in livers were observed by histopathology - areas of necrosis and collagen septa were identified. Parasites caused nodules with necrosis on the periphery of lesions, and collagen fibres were also observed in stomachs. Collagen septa may be caused by antigenic remains of the parasite. Further immunological studies are necessary to verify that stimulation is caused by these factors.

  6. New Method for Simultaneous Species-Specific Identification of Equine Strongyles (Nematoda, Strongylida) by Reverse Line Blot Hybridization▿

    Science.gov (United States)

    Traversa, Donato; Iorio, Raffaella; Klei, Thomas R.; Kharchenko, Vitaliy A.; Gawor, Jakub; Otranto, Domenico; Sparagano, Olivier A. E.

    2007-01-01

    The ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and simultaneously discriminated from each other and from three large strongyles (Strongylus edentatus, Strongylus equinus, and Strongylus vulgaris). This assay will enable the accurate and rapid identification of equine cyathostomins irrespective of their life cycle stage, opening important avenues for a better understanding of their biology and epidemiology and of the pathogenesis of cyathostomin-associated disease. In particular, this RLB method promises to be a powerful diagnostic tool to determine the roles of individual species in the pathogenesis of mixed infections and to elucidate some aspects of cyathostominosis. Also, it could represent a basic step toward the development of a rapid and simple molecular test for the early detection of drug-resistant genotypes of horse strongyle species. PMID:17626168

  7. Role of lipids in the transmission of the infective stage (L3) of Strongylus vulgaris (Nematoda: Strongylida).

    Science.gov (United States)

    Medica, D L; Sukhdeo, M V

    1997-10-01

    Infective larvae (L3) of Strongylus vulgaris have limited energy stores for host finding and for infection. For transmission to occur, the larvae must have sufficient energy to (a) migrate onto grass, where they are ingested by their equine host (host finding), and (b) penetrate into the host gut. This study is designed to test the hypothesis that L3 larvae of S. vulgaris partition their energy stores between locomotory activity (used in host finding) and infection activity (penetration). Chronic locomotory activity was stimulated by incubating S. vulgaris L3 larvae at a constant temperature (38 C). After 8 days of treatment, locomotory activity ceased (exhaustion). Exhausted L3 larvae had significantly decreased total lipid when compared to controls (P vulgaris L3 larvae are comprised of 9 fatty acids, some of which are depleted in exhausted worms (14:0, 14:1, 16:0, 16:1, 18:1, 18:2), whereas others (18:0, 20:4, 24:0) remain unchanged. These data suggest that specific fatty acids provide the energy source for locomotory activity in S. vulgaris. Exhausted L3 larvae were also less able to penetrate host cecal tissue in in vitro penetration assays when compared to controls (P vulgaris L3 larvae partition their energy stores between host-finding and infection activities. A comparison of lipid storage profiles in the L3 larvae of 4 nematode species with similar transmission strategies (S. vulgaris, Strongylus edentatus, Strongylus equinus, and Haemonchus contortus) revealed similarities in the fatty acid composition of these species. These data suggest a relationship between transmission patterns and energy storage strategies in the L3 larvae of nematode parasites of vertebrates.

  8. Investigating the Strongyle Populations, with Emphasis on Strongylus vulgaris (Nematoda: Strongylidae in Romanian Horses, Based on Larval Cultures

    Directory of Open Access Journals (Sweden)

    Marius Catalin BUZATU

    2017-11-01

    Full Text Available This study aimed to investigate the presence of Strongylus vulgaris on different horse establishments in Romania. A total number of 380 horses were enrolled in the study, representing: three stud farms (n=156; four sport/recreational establishments (n=75; and working horses (n=149. Overall, 106 premises/yards from 17 localities in 9 counties from North-Eastern, Center and Southern Romania were visited. Fresh fecal sample were collected from individual horses and were analyzed by a modified McMaster method for fecal strongyle egg per gram counting. Further, larval cultures were performed for morphological identification of S. vulgaris third stage larvae. Overall, of the investigated horses from studs, sport/recreational units, and working horses, 84.60%, 44.0%, and 79.20%, respectively were positive for strongyle. On coprocultures, cyathostomin-larvae were the most prevalent in all horse-establishment types, while S. vulgaris larvae were found only in working horses (12.1%; n=18. Other strongylin species have been identified, namely S. equinus, S. edentatus, Oesophagodontus robustus, and Triodontophorus spp. all in working horses, and Craterostomum acuticaudatum, in both working horses and horses for sport units. However, further studies are planned by using more sensitive technique for monitoring of S. vulgaris in Romanian horse farms.

  9. Two new species of Parapharyngodon parasites of Sceloporus pyrocephalus, with a key to the species found in Mexico (Nematoda, Pharyngodonidae).

    Science.gov (United States)

    Garduño-Montes de Oca, Edgar Uriel; Mata-López, Rosario; León-Règagnon, Virginia

    2016-01-01

    Two new species of Parapharyngodon collected from the intestine of the Mexican boulder spiny lizard Sceloporus pyrocephalus are described. This study increases to 49 the number of valid species assigned to Parapharyngodon worldwide, 11 of them distributed in Mexico. Males of the two new species share the presence of four pairs of caudal papillae, an anterior echinate cloacal lip and the presence of lateral alae; however, both differ from each other in lateral alae extension and echinate cloacal anterior lip morphology. Females of both species have a prebulbar uterus and eggs shell punctuate with pores, characteristics shared with few other species of Parapharyngodon. Both new species differ from other congeneric species in the papillar arrangement, the anterior cloacal lip morphology, the lateral alae extension and total length/spicule ratio. A taxonomic key for the species of Parapharyngodon distributed in Mexico is provided.

  10. Spinitectus mexicanus n. sp. (Nematoda : Cystidicolidae) from the intestine of the freshwater fish Heterandria bimaculata in Mexico.

    Science.gov (United States)

    Caspeta-Mandujano, J M; Moravec, F; Salgado-Maldonado, G

    2000-02-01

    A new nematode, Spinitectus mexicanus n. sp., is described on the basis of the specimens recovered from the intestine of Heterandria bimaculata (Heckel) (Poeciliidae, Cyprinodontiformes) from 3 rivers of the Papaloapan River basin (type locality La Basura River), Los Tuxtlas, Veracruz State, Mexico. It differs from its congeners mainly in having the spination of the cuticle separated into 4 longitudinal sectors, each with posteriorly diminishing numbers of larger spines at the anterior part of body. It is the first species of Spinitectus described from a poeciliid fish and the second reported from freshwater fishes in Mexico.

  11. Chapiniella variabilis (Nematoda parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae in the state of Piauí

    Directory of Open Access Journals (Sweden)

    Simone Mousinho Freire

    Full Text Available Abstract Chapiniella variabilis (Chapin, 1924, a strongylid nematode, was collected parasitizing the large intestine of the tortoises Chelonoidis carbonarius (Spix, 1824 (Cc and C. denticulatus (Linnaeus, 1766 (Cd in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. The taxonomic identification was based on morphological and morphometric features, using bright-field and scanning electron microscopy. The present study adds new observations on the morphology, mainly relating to the mouth papillae, external and internal leaf-crown elements, excretory pore, deirids and male and female posterior end. The parasitic indices of prevalence (P, mean intensity (MI, mean abundance (MA and range of infection (RI of C. variabilis in these two tortoise species were: P = 100%, MI = 833.3, MA = 833.3, RI = 500-1,500 (Cc; P = 100%, MI = 472.2, MA = 472.2, RI = 333-500 (Cd. This record expands occurrences of C. variabilis to a new host, C. carbonarius, and to another state in Brazil, in the Neotropical region of South America. Adjustment to host management with the aim of improving hygiene and health conditions is suggested.

  12. Ancylostoma ailuropodae sp. n. (Nematoda: Ancylostomatidae), a new hookworm parasite isolated from wild giant pandas in Southwest China

    Science.gov (United States)

    Hookworms belonging to the genus Ancylostoma cause ancylostomiasis, a disease of considerable concern in humans and domestic and wild animals. Molecular and epidemiological data support evidence for the zoonotic potential among species of Ancylostoma where transmission to humans is facilitated by ra...

  13. Monoxenic culture of the slug parasite Phasmarhabditis hermaphrodita (Nematoda : Rhabditidae) with different bacteria in liquid and solid phase

    OpenAIRE

    Wilson, M.J.; Glen, D.M.; Pearce, J.D.; Rodgers, P.B.

    1995-01-01

    Seize isolats bactériens, représentant treize espèces, ont été testés pour leur capacité à maintenir la croissance de #Phasmarhabditis hermaphrodita$ en culture monoxénique sur agar, en boîte de Petri. Tous les isolats permettent la croissance de #P. hermaphrodita$, mais la capacité de reproduction varie considérablement suivant les différentes bactéries. Cinq espèces de bactéries ayant permis une croissance vigoureuse de #P. hermaphrodita$ ont été utilisées pour son élevage en milieu liquide...

  14. Microscopic and molecular characterization of Hepatozoon domerguei (Apicomplexa) and Foleyella furcata (Nematoda) in wild endemic reptiles from Madagascar.

    Science.gov (United States)

    Maia, João P; Crottini, Angelica; Harris, David James

    2014-01-01

    Madagascar is one of the world's top twelve "megadiversity" hot spots hosting unique and threatened flora and fauna. Parasites are a major component of biodiversity but remain largely uncharacterized in wildlife. In this study we combine microscopic and molecular assessment of hemoparasites in endemic reptile species from Madagascar. We detected three distinct parasites: the apicomplexans Hepatozoon and Sarcocystis, and filarial nematodes. The prevalence and intensity of these apicomplexans were low overall, while microfilarial infections in chameleons were relatively high. We detected mixed infections of two Hepatozoon haplotypes in Madagascarophis colubrinus, and of Hepatozoon and microfilariae in a Furcifer sp. Phylogenetic analyses of Hepatozoon showed evidence of prey-predator transmission, with identical sequences found in the snakes M. colubrinus and Ithycyphus oursi, and their prey Furcifer sp. Based on previous studies regarding the life cycle of Hepatozoon domerguei Landau, Chabaud, Michel, and Brygoo, 1970 in these hosts and due to their morphological similarity, we propose that this Hepatozoon haplotype is Hepatozoon domerguei. Future studies, including the examination of invertebrate hosts, are needed to verify this preliminary taxonomic identification. A distinct hemogregarine haplotype was found in Oplurus sp., which displayed morphologically different gametocytes, some of which were apparently inside leukocytes. The Sarcocystis identified from Tracheloptychus petersi was identical to that reported in a North African snake, indicating that the same lineage is found in geographically distinct regions. By combining morphological and genetic information, Foleyella furcata (Linstow, 1899) filarial nematodes were identified in several Furcifer chameleons. This study provides insights into the distribution, diversity and host-parasite interactions of hemoparasites in wild reptile populations from Madagascar. © J.P. Maia et al., published by EDP Sciences, 2014.

  15. Cucullanid nematodes (Nematoda: Cucullanidae) from deep-sea marine fishes off New Caledonia, including Dichelyne etelidis n. sp

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.

    2011-01-01

    Roč. 78, č. 2 (2011), s. 95-108 ISSN 0165-5752 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cucullanidae * marine fish * New Caledonia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.250, year: 2011

  16. Capillaria (Hepatocapillaria) cichlasomae (Nematoda: Capillariidae) from the liver of the cichlid fish Cichlasoma urophthalmus from Yucatan, Mexico.

    Science.gov (United States)

    Moravec, F; Scholz, T; Mendoza Franco, E

    1995-01-01

    Capillaria (Hepatocapillaria) cichlasomae sp. n., parasitic in the liver of the cichlid Cichlasoma urophthalmus (Günther) from a small freshwater lake ("aguada") Xpoc in Yucatan, Mexico, is described. The parasite is characterized mainly by its small body size (male 1.8 mm, female 4.5 mm), the structure of the stichosome (markedly short stichocytes in one row) and the male (the presence of a pair of small subventral postanal papillae) and female (anus distinctly subterminal) caudal ends, and by the size and structure of the spicule (spicule 0.068-0.085 mm long, with marked transverse grooves on surface) and eggs (size 0.053-0.058 x 0.023 mm, with protruding polar plugs). This is the second known Capillaria species from the liver of fish and the first one from the liver of a freshwater fish.

  17. Two new species of Quasithelazia Maplestone, 1932 (Nematoda: Acuariidae) from Malaysia, with an amended diagnosis and review of the genus.

    Science.gov (United States)

    Mutafchiev, Yasen; Mariaux, Jean; Georgiev, Boyko B

    2014-06-01

    Quazithelazia rostrata n. sp. from Ceyx erithaca (L.) (type-host) and Alcedo euryzona Temminck (Coraciiformes, Alcedinidae) and Q. alata n. sp. from Enicurus ruficapillus Temminck (Passeriformes, Muscicapidae) are described from vicinities of Gombak Biological Station, Selangor, Malaysia; both species are parasitic under the koilin lining of the gizzard. Paratypes of Schistogendra pelargopsis Nandi, De & Majumdar, 1985, a parasite of Pelargopsis capensis (L.) (Alcedinidae) from India, are redescribed and the species is recognised as a junior synonym of the type-species of Quasithelazia, Q. tenuis Maplestone, 1932 (new synonymy), a species originally described from Halcyon smyrnensis (L.) (Alcedinidae) in India. An amended diagnosis of the genus Quasithelazia Maplestone, 1932 is proposed. Currently, this genus includes eight species occurring in the Old World, six of them parasitic in kingfishers (Alcedinidae) and two species parasitic in flycatchers (Muscicapidae). These include, inter alia, Q. halcyoni n. comb. for Viktorocara halcyoni Ryzhikov & Khokhlova, 1964 from Halcyon smyrnensis and H. pileata (Boddaert) in Vietnam and the Russian Far East, Q. microcordonis n. comb. for Rusguniella microcordonis Schmidt & Kuntz, 1971 from Halcyon coromanda major (Temminck & Schlegel) in Taiwan and Q. multipapillata n. comb. for Schistogendra multipapillata Zhang, 1993 from Tarsiger cyanurus (Pallas) (Muscicapidae) in China. Comparative morphological data for Quasithelazia spp. are presented. Schistogendra oligopapillata Zhang & An, 2002 from domestic ducks in China is considered a species incertae sedis.

  18. Nematodes of amphibians from Java, Indonesia, with a description of new species, Meteterakis wonosoboensis n. sp. (Nematoda : Heterakoidea).

    Science.gov (United States)

    Purwaningsih, Endang; Dewi, Kartika; Hasegawa, Hideo

    2015-06-24

    During a survey on the parasites of amphibians of Indonesia, toads (30 Bufo melanostictus) and 246 frogs (213 Fejervarya cancrivora, 11 F. limnocharis, 22 Rana macrodon from West Java and 68 F. cancrivora from Central Java) were examined for parasitic nematodes. Three species of nematodes were found and described, i.e. Meteterakis wonosoboensis n. sp. from Fejervaria cancrivora; Meteterakis japonica from Bufo melanostictus, F. cancrivora and F. limnocharis; and Chabaudus sp. from F. cancrivora, F. limnocharis and Rana macrodon. Meteterakis wonosoboensis n. sp. is distinguished from other species of the genus by the length and shape of spicules, the number of caudal papillae, the presence of gubernaculum in male and the presence of vulval flap in female. Bufo melanostictus and Java are recorded as new host and locality for M. japonica, respectively.

  19. Morphological variability and molecular identification of Uncinaria spp. (Nematoda: Ancylostomatidae) from grizzly and black bears: new species or phenotypic plasticity?

    Science.gov (United States)

    Catalano, Stefano; Lejeune, Manigandan; van Paridon, Bradley; Pagan, Christopher A; Wasmuth, James D; Tizzani, Paolo; Duignan, Pádraig J; Nadler, Steven A

    2015-04-01

    The hookworms Uncinaria rauschi Olsen, 1968 and Uncinaria yukonensis ( Wolfgang, 1956 ) were formally described from grizzly ( Ursus arctos horribilis) and black bears ( Ursus americanus ) of North America. We analyzed the intestinal tracts of 4 grizzly and 9 black bears from Alberta and British Columbia, Canada and isolated Uncinaria specimens with anatomical traits never previously documented. We applied morphological and molecular techniques to investigate the taxonomy and phylogeny of these Uncinaria parasites. The morphological analysis supported polymorphism at the vulvar region for females of both U. rauschi and U. yukonensis. The hypothesis of morphological plasticity for U. rauschi and U. yukonensis was confirmed by genetic analysis of the internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA. Two distinct genotypes were identified, differing at 5 fixed sites for ITS-1 (432 base pairs [bp]) and 7 for ITS-2 (274 bp). Morphometric data for U. rauschi revealed host-related size differences: adult U. rauschi were significantly larger in black bears than in grizzly bears. Interpretation of these results, considering the historical biogeography of North American bears, suggests a relatively recent host-switching event of U. rauschi from black bears to grizzly bears which likely occurred after the end of the Wisconsin glaciation. Phylogenetic maximum parsimony (MP) and maximum likelihood (ML) analyses of the concatenated ITS-1 and ITS-2 datasets strongly supported monophyly of U. rauschi and U. yukonensis and their close relationship with Uncinaria stenocephala (Railliet, 1884), the latter a parasite primarily of canids and felids. Relationships among species within this group, although resolved by ML, were unsupported by MP and bootstrap resampling. The clade of U. rauschi, U. yukonensis, and U. stenocephala was recovered as sister to the clade represented by Uncinaria spp. from otariid pinnipeds. These results support the absence of strict host-parasite co-phylogeny for Uncinaria spp. and their carnivore hosts. Phylogenetic relationships among Uncinaria spp. provided a framework to develop the hypothesis of similar transmission patterns for the closely related U. rauschi, U. yukonensis, and U. stenocephala.

  20. Additional observations on Philometra spp. (Nematoda: Philometridae) in marine fishes off Iraq, with the description of two new species

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Ali, A. H.

    2014-01-01

    Roč. 87, č. 3 (2014), s. 259-271 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : systematic status * India * Philometridae Subject RIV: EA - Cell Biology Impact factor: 1.336, year: 2014

  1. Molecular systematics of pinniped hookworms (Nematoda: Uncinaria): species delimitation, host associations and host-induced morphometric variation.

    Science.gov (United States)

    Nadler, Steven A; Lyons, Eugene T; Pagan, Christopher; Hyman, Derek; Lewis, Edwin E; Beckmen, Kimberlee; Bell, Cameron M; Castinel, Aurelie; Delong, Robert L; Duignan, Padraig J; Farinpour, Cher; Huntington, Kathy Burek; Kuiken, Thijs; Morgades, Diana; Naem, Soraya; Norman, Richard; Parker, Corwin; Ramos, Paul; Spraker, Terry R; Berón-Vera, Bárbara

    2013-12-01

    Hookworms of the genus Uncinaria have been widely reported from juvenile pinnipeds, however investigations of their systematics has been limited, with only two species described, Uncinaria lucasi from northern fur seals (Callorhinus ursinus) and Uncinaria hamiltoni from South American sea lions (Otaria flavescens). Hookworms were sampled from these hosts and seven additional species including Steller sea lions (Eumetopias jubatus), California sea lions (Zalophus californianus), South American fur seals (Arctocephalus australis), Australian fur seals (Arctocephalus pusillus), New Zealand sea lions (Phocarctos hookeri), southern elephant seals (Mirounga leonina), and the Mediterranean monk seal (Monachus monachus). One hundred and thirteen individual hookworms, including an outgroup species, were sequenced for four genes representing two loci (nuclear ribosomal DNA and mitochondrial DNA). Phylogenetic analyses of these sequences recovered seven independent evolutionary lineages or species, including the described species and five undescribed species. The molecular evidence shows that U. lucasi parasitises both C. ursinus and E. jubatus, whereas U. hamiltoni parasitises O. flavescens and A. australis. The five undescribed hookworm species were each associated with single host species (Z. californianus, A. pusillus, P. hookeri, M. leonina and M. monachus). For parasites of otarids, patterns of Uncinaria host-sharing and phylogenetic relationships had a strong biogeographic component with separate clades of parasites from northern versus southern hemisphere hosts. Comparison of phylogenies for these hookworms and their hosts suggests that the association of U. lucasi with northern fur seals results from a host-switch from Steller sea lions. Morphometric data for U. lucasi shows marked host-associated size differences for both sexes, with U. lucasi individuals from E. jubatus significantly larger. This result suggests that adult growth of U. lucasi is reduced within the host species representing the more recent host-parasite association. Intraspecific host-induced size differences are inconsistent with the exclusive use of morphometrics to delimit and diagnose species of Uncinaria from pinnipeds. Copyright © 2013 Australian Society for Parasitology Inc. All rights reserved.

  2. The infective larva of Litomosoides yutajensis Guerrero et al., 2003 (Nematoda: Onchocercidae, a Wolbachia-free filaria from bat

    Directory of Open Access Journals (Sweden)

    Guerrero R.

    2006-06-01

    Full Text Available The infective larva of Litomosoides yutajensis Guerrero et al., 2003, a parasite of the bat Pteronotus parnellii, is described; it is distinct from congeneric infective larvae by the absence of caudal lappets. The life cycles of five other species of Litomosoides are known; three are parasites of rodents, one of a marsupial and one of a bat. As with these species, the experimental vector of L. yutajensis used was the macronyssid mite Ornithonyssus bacoti. In nature, the main vectors are probably other macronyssids but transmission by O. bacoti, with its large host-range, could account for the characteristic host-switchings in the evolution of Litomosoides. Unlike the murine model L. sigmodontis Chandler, 1931, L. yutajensis is devoid of the endosymbiontic bacteria Wolbachia and may be of great interest.

  3. First report of Litomosa spp. (Nematoda: Filarioidea from malagasy bats; review of the genus and relationships between species

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    Martin C.

    2006-03-01

    Full Text Available The presence of the filarial genus Litomosa in Malagasy bats is demonstrated by the finding of L. goodmani n. sp. from Miniopterus gleni and Litomosa sp. (male unknown from M. manavi, both in the Special Reserve of Ankarana. These materials are compared to the 22 Litomosa species, including two Indian species originally placed in the genus Litomosoides, L. fotedari (Gupta & Trivedi, 1989 n. comb. and L. tewarii (Gupta & Trivedi, 1989 n. comb., and the new taxon L. seurati n. sp. (= L. beaucournui Bain, 1966 pro parte, type-host Rhinolophus ferrum-equinum, Algeria, distinguished by the narrow area rugosa and the female caudal extremity with two conspicuous points, instead of several small ones. The Malagasy material belongs to a group of species close to the type, L. filaria, which have a male area rugosa composed of cuticular bosses and microfilariae folded within the sheath, and which are parasitic in Vespertilionidae, Hipposideridae and Rhinolophidae from Africa and Europe. The two Malagasy species resemble L. seurati n. sp., L. beshkovi Jancev, 1971, L. chiropterum Ortlepp, 1932, L. adami Petit, 1980 and L. ottavianii Lagrange et Bettini, 1948, with the enlarged third segment of the buccal capsule. L. goodmani n. sp. is distinct with its small size and female caudal extremity with a single point, which is suppressed in old mature worms; the females of Litomosa sp. have two conical points. Relationships among Litomosa species appear to be dependent upon both the chiropteran host groups and the geographical region.

  4. Redescription of Camallanus hypophthalmichthys Dogel and Akhmerov, 1959 (Nematoda: Camallanidae) and its first record from fishes in China

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Nie, P.; Wang, G.

    2004-01-01

    Roč. 90, č. 6 (2004), s. 1463-1467 ISSN 0022-3395 R&D Projects: GA MŠk ME 424 Grant - others:National Natural Science Foundation of China(CN) 30025035 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematodes * Camallanus * freshwater fishes Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.439, year: 2004

  5. Occurrence of Philometra lateolabracis (Nematoda: Philometridae) in the gonads of marine perciform fishes in the Mediterranean region

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Glamuzina, B.; Marino, G.; Merella, P.; Di Cave, D.

    2003-01-01

    Roč. 53, č. 3 (2003), s. 267-269 ISSN 0177-5103 R&D Projects: GA ČR GA524/03/0061 Institutional research plan: CEZ:AV0Z6022909 Keywords : parasitic nematode * Philometra lateolabracis * marine fish Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.263, year: 2003

  6. Larval Pseudoproleptus sp. (Nematoda: Cystidicolidae) found in the Amazon river prawn Macrobrachium amazonicum (Decapoda: Palaemonidae) in Brazil

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Santos, C. P.

    2009-01-01

    Roč. 95, č. 3 (2009), s. 634-638 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Pseudoproleptus * Macrobrachium * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.195, year: 2009

  7. Pengaruh Berbagai Ekstrak Metanol Tumbuhan terhadap Mortalitas Juvenil Instar-2 dan Penetasan Telur Nematoda Sista Kentang (Globodera rostochiensis

    Directory of Open Access Journals (Sweden)

    Iis Nur Asyiah

    2005-07-01

    Full Text Available The research was conducted to know the influence of methanol extracts from 19 plant species to the second-stage juvenil (J2 mortality and to the hatching of the potato cyst nematode, Globodera rostochiensis, the new pest of potato in Indonesia. The research was conducted by using complete random design, where each treatment was repeated 3 times. Cyst hatching was conducted by using ZnSO4 10-2 M. Data obtained to be analyzed with the ANOVA and continued with the DMRT 5%. The result indicated that from 19 species of examined plant, clove flower and leaf methanol extract with concentration 10.000 ppm inflicted 100% and 96,7% J2 mortality and others inflicted <50% 12 mortality. Clove flower and leaf methanol extract permanently inhibited of cyst hatching, methanol extract of Andrograpis puniculata, Capsium frutescens, Chrysanthemum sp., and Allium sativum inhibited of cyst hatching non permanently, while other plants extract methanol didn't inhibit of cyst hatching.

  8. Spinicauda sumatrana sp. nov. (Nematoda: Heterakidae) from Ludeking's Crested Dragon, Lophocalotes ludekingi (Agamidae), from the Bukit Barisan Range of Sumatra.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R; Harvey, Michael B

    2017-09-26

    Spinicauda sumatrana sp. nov. is described from the large intestine of Ludeking's Crested Dragon, Lophocalotes ludekingi (Agamidae) from the Bukit Barisan Range of Sumatra. The new species is assigned to Spinicauda based on the presence of off-set lips in both male and female and a ventrally directed sucker combined with the absence of caudal alae in males. It is the 17th species assigned to the genus and differs from all congeners in the combination of spicule length, presence of a gubernaculum, presence of a tail filament and fourteen pairs of caudal papillae.

  9. Meloidogyne luci n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitising different crops in Brazil, Chile and Iran

    NARCIS (Netherlands)

    Carneiro, R.M.D.G.; Correa, V.R.; Almeida, M.R.A.; Gomes, A.C.M.M.; Deimi, A.M.; Castagnone-Sereno, P.; Karssen, G.

    2014-01-01

    A new root-knot nematode parasitising vegetables, flowers and fruits in Brazil, Iran and Chile, is described as Meloidogyne luci n. sp. The female has an oval to squarish perineal pattern with a low to moderately high dorsal arc and without shoulders, similar to M. ethiopica. The female stylet is

  10. Pancreatitis associated with the helminth Serpinema microcephalus (Nematoda: Camallanidae) in exotic Red-Eared Slider Turtles (Trachemys scripta elegans)

    Czech Academy of Sciences Publication Activity Database

    Hidalgo-Vila, J.; Martínez-Silvestre, A.; Ribas, Alexis; Casanova, J. C.; Pérez-Santigosa, N.; Díaz-Paniagua, C.

    2011-01-01

    Roč. 47, č. 1 (2011), s. 201-205 ISSN 0090-3558 Institutional research plan: CEZ:AV0Z60930519 Keywords : helminth * invasive exotic turtles * pancreatitis * reptiles Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.079, year: 2011 http://www.jwildlifedis.org/cgi/reprint/47/1/201

  11. Redescriptions and comments on the validity of Acuaria subula and A. skrjabini (Nematoda, Spirurida, Acuariidae), parasites of passerine birds.

    Science.gov (United States)

    Mutafchiev, Yasen; Kontrimavichus, Vytautas L; Georgiev, Boyko B

    2013-09-01

    Acuaria subula (Dujardin, 1845) is redescribed by light microcopy (LM) and scanning electron microscopy (SEM) on the basis of specimens from its type host, Erithacus rubecula (Passeriformes, Muscicapidae), from Curonian Spit (Kaliningradskaya Oblast', Russia) and Bulgaria. Acuaria skrjabini (Ozerskaya, 1926) is redescribed by LM and SEM on the basis of specimens from Passer domesticus (type host) and P. hispaniolensis (Passeriformes, Passeridae) from Bulgaria. Contrary to previous opinions recognizing A. skrjabini as a junior synonym of A. subula, the present study confirms that they are distinct species. They can be distinguished on the basis of the ratio between the length of cordons and the body length, the ratio between the length of muscular oesophagus and glandular oesophagus, and the ratio between the total length of oesophagus and the body length. In addition, the plates forming the cordons in these two species exhibit different morphological characters. Another difference between these two species is associated with the particular irregular mosaic ornamentation of the cuticle on the ventral and lateral sides of body around the region of vulva of A. subula and its absence in A. skrjabini. Data on their host and geographical ranges are surveyed. The type series of Acuaria buttnerae Chabaud et Petter, 1961, described as a parasite of Calandrella brachydactyla (Passeriformes, Alaudidae) in France, is re-examined; the latter species is recognized as a junior synonym of A. skrjabini (new synonymy).

  12. Effects of associated bacteria on the pathogenicity and reproduction of the insect-parasitic nematode Rhabditis blumi (Nematoda: Rhabditida).

    Science.gov (United States)

    Park, Hae Woong; Kim, Yong Ook; Ha, Jae-Seok; Youn, Sung Hun; Kim, Hyeong Hwan; Bilgrami, Anwar L; Shin, Chul Soo

    2011-09-01

    Three bacteria, Alcaligenes faecalis , Flavobacterium sp., and Providencia vermicola , were isolated from dauer juveniles of Rhabditis blumi . The pathogenic effects of the bacteria against 4th instar larvae of Galleria mellonella were investigated. Providencia vermicola and Flavobacterium sp. showed 100% mortality at 48 h after haemocoelic injection, whereas A. faecalis showed less than 30% mortality. Dauer juveniles showed 100% mortality against G. mellonella larvae, whereas axenic juveniles, which do not harbor associated bacteria, exhibited little mortality. All of the associated bacteria were used as a food source for nematode growth, and nematode yield differed with bacterial species. Among the bacterial species, P. vermicola was most valued for nematode yield, showing the highest yield of 5.2 × 10(4) nematodes/mL in the plate. In bacterial cocultures using two of the three associated bacteria, one kind stimulated the other. The highest total bacterial yield of 12.6 g/L was obtained when the inoculum ratio of P. vermicola to A. faecalis was 10:1. In air-lift bioreactors, the nematode growth rate increased with an increasing level of dissolved oxygen. The maximum nematode yield of 1.75 × 10(5) nematodes/mL was obtained at 192 h with an aeration rate of 6 vvm.

  13. Three new species of the genus Oswaldocruzia Travassos, 1917 (Nematoda, Trichostrongylina, Molineoidea parasites of Enyalius spp. (Iguanidae from Brazil

    Directory of Open Access Journals (Sweden)

    Durette-Desset M.C.

    2006-06-01

    Full Text Available Three new species of the genus Oswaldocruzia Travassos, 1917 belonging to the sub-family Molineinae are described from the stomach and/or the small intestine of Enyalius spp. from Brazil. They belong to group 6 of Ben Slimane, Chabaud & Durette- Desset (1996. In this group they share along with O. peruensis Ben Slimane, Verhaag & Durette-Desset, 1995, a parasite of Iguanidae from Peru the followings linked characters: (i a caudal bursa of type II; (ii cervical alae present; (iii undulated cuticular ridges. The Peruvian species differs from the Brasilian species by the absence of a strut in the cervical alae, by a small number of cuticular ridges at mid-body and by a spicular fork with a ramified inner twig. Oswaldocruzia fredi n. sp., a parasite of the stomach and the small intestine of Enyalius iheringii, mainly differs from the two other species by the absence of the oesophageal ventral cuticular ridges. Oswaldocruzia benslimanei n. sp., a parasite of the small intestine of Enyalius bilineatus, differs from Oswaldocruzia burseyi n. sp., a parasite of the stomach of Enyalius perditus, by the division of the fork at 23.4 % of spicule length (versus 32 %, and the length of the blade longer than the fork. Oswaldocruzia subauricularis sensu Freitas, 1955 nec Rudolphi, 1819 and O. mazzai sensu Vicente, 1981 nec Travassos, 1935 should be considered as species inquirendae.

  14. The first record of Molineus patens (Dujardin, 1845) (Nematoda, Molineidae) in the ermine (Mustela erminea L.) in Poland.

    Science.gov (United States)

    Popiołek, Marcin; Jarnecki, Hubert; Łuczyński, Tomasz

    2009-01-01

    A single specimen of the nematode Molineus patens (Dujardin, 1845) was isolated from the intestine of the ermine (Mustela erminea L.) found dead on a road in Lubuskie voivodeship (Western Poland) in July 2008. Since this is the first record of the parasite in the ermine from Poland, description, biometrical data and figures are given.

  15. New species of Oswaldocruzia (Nematoda: Molineidae) and other helminths in Bolitoglossa subpalmata (Caudata:Plethodontidae) from Costa Rica.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R

    2011-04-01

    Oswaldocruzia cartagoensis n. sp. (Strongylida: Molineidae) from the intestines of Bolitoglossa subpalmata (Caudata: Plethodontidae) is described and illustrated. Oswaldocruzia cartagoensis n. sp. represents the 86 th species assigned to the genus and the 39th species from the Neotropical region. It is most similar to the Neotropical species of the genus that possess type I bursa, i.e., Oswaldocruzia bonsi , Oswaldocruzia brasiliensis , Oswaldocruzia lopesi , Oswaldocruzia neghmei , and Oswaldocruzia vitti . Of these, O. bonsi, O. brasiliensis, and O. neghmei lack cervical alae, rib 4 in individuals of O. vitti reaches the edge of the bursal membrane, species of O. lopesi and O. cartagoensis can be separated on the basis of spicule structure, the blade in O. lopesi is bifurcate, and that of O. cartagoensis terminates in 6-8 fine points. In addition to the new species of Oswaldocruzia, Cosmocera parva, Cosmocera podicipinus, and acanthocephalan cystacanths were also found.

  16. Host specificity and basic ecology of Mammomonogamus (Nematoda, Syngamidae) from lowland gorillas and forest elephants in Central African Republic

    Czech Academy of Sciences Publication Activity Database

    Červená, B.; Vallo, Peter; Pafčo, B.; Jirků, K.; Jirků, M.; Petrželková, Klára Judita; Todd, A.; Turkalo, A. K.; Modrý, D.

    2017-01-01

    Roč. 144, č. 8 (2017), s. 1016-1025 ISSN 0031-1820 R&D Projects: GA ČR GA15-05180S Institutional support: RVO:68081766 Keywords : 18S rDNA * cox1 * Dzanga-Sangha Protected Areas * haplotype * interspecies transmission * Loxodonta cyclotis * Gorilla gorilla gorilla Subject RIV: EG - Zoology OBOR OECD: Parasitology Impact factor: 2.713, year: 2016

  17. Host specificity and basic ecology of Mammomonogamus (Nematoda, Syngamidae) from lowland gorillas and forest elephants in Central African Republic

    Czech Academy of Sciences Publication Activity Database

    Červená, B.; Vallo, P.; Pafčo, B.; Jirků-Pomajbíková, Kateřina; Jirků, Miloslav; Petrželková, Klára Judita; Todd, A.; Turkalo, A. K.; Modrý, David

    2017-01-01

    Roč. 144, č. 8 (2017), s. 1016-1025 ISSN 0031-1820 Institutional support: RVO:60077344 Keywords : 18S rDNA * cox1 * Dzanga-Sangha Protected Areas * haplotype * interspecies transmission * Loxodonta cyclotis * Gorilla gorilla gorilla Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 2.713, year: 2016

  18. Cucullanus pargi sp. n. (Nematoda: Cucullanidae) from the grey snapper Lutjanus griseus off the southern coast of Quintana Roo, Mexico.

    Science.gov (United States)

    González-Solís, David; Tuz-Paredes, Vielka M; Quintal-Loria, Miguel A

    2007-09-01

    A new nematode species, Cucullanus pargi sp. n., is described from the intestine and pyloric caeca of the grey snapper, Lutjanus griseus (Linnaeus), off the southern Quintana Roo coast, Mexico. This species shows similar morphological features as cucullanids occurring in marine and brackish-water fishes; however, it differs from all other species in the length of spicules, arrangement and number of caudal papillae, position of the excretory pore and deirids. Cucullanus pargi is the third species of this genus described from fishes in Mexico and the second one from Mexican marine fishes.

  19. Dichelyne (Cucullanellus) romani n. sp (Nematoda: Cucullanidae) in notacanthid fishes from the Northeast Atlantic and Western Mediterranean

    Czech Academy of Sciences Publication Activity Database

    Isbert, W.; Esteban Montero, F.; Carrasson, M.; González-Solís, David

    2015-01-01

    Roč. 91, č. 1 (2015), s. 35-47 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : ribosomal dna * marine fishes * pisces * sea * Ascaridoidea * Sciaenidae * morphology * tornquist * parasites * Mexico Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.316, year: 2015

  20. Redescription of Paraleptus chiloscyllii Yin et Zhang, 1983 (Nematoda: Physalopteridae) from the Arabian carpetshark Chiloscyllium arabicum (Chondrichthyes: Hemiscylliidae) off Iraq

    Czech Academy of Sciences Publication Activity Database

    González-Solís, David; Ali, A. H.

    2015-01-01

    Roč. 60, č. 4 (2015), s. 759-766 ISSN 1230-2821 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Arabian Gulf * elasmobranchs * nematode Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.293, year: 2015

  1. Two new genera and five new species of Selachinematidae (Nematoda, Chromadorida from the continental slope of New Zealand

    Directory of Open Access Journals (Sweden)

    Daniel Leduc

    2013-11-01

    Full Text Available Two new genera and five new species of Selachinematidae are described from the New Zealand upper continental slope (350-1240 m depth. Synonchiella rotundicauda sp. nov. is characterised by cephalic setae 0.25 cbd long, mandibles each with two pairs of hooks and two wing-like projections laterally, eight cup-shaped pre-cloacal supplements and short rounded tail. Pseudocheironchus gen. nov. is similar to Cheironchus, but differs from the latter in having a cuticle without lateral differentiation, cephalic setae only slightly longer than the outer labial sensillae, and a posterior buccal cavity with three equal mandibles. Pseudocheironchus ingluviosus gen. et sp. nov. is characterised by mandibles with eight blunt teeth, multispiral amphideal fovea with five turns, and a short rounded tail. Males of this new species with 17-19 cup-shaped pre-cloacal supplements. Males of the genus Cobbionema are described for the first time; C. trigamma sp. nov. is characterised by four long cephalic setae and six smaller outer labial setae in one circle, six rhabdions surrounding the anterior buccal cavity, each with two pairs of pointed projections at their posterior extremities, posterior buccal cavity widening posteriorly, with three pairs of rhabdions fused posteriorly and widening anteriorly, males with two testes pointing anteriorly and with reflexed posterior testis, and no pre-cloacal supplements. Gammanema agglutinans sp. nov. is characterised by a short, stout body often covered in adhering mucus and detritus, cuticle with minute spines, leaf-shaped somatic setae with ducts, sexual dimorphism in the shape of the amphideal fovea (loop-shaped in males and spiral in females, posterior buccal cavity with three pairs of broad, column-shaped rhabdions fused anteriorly, intestine cells with orange-brown granules, and small tubular pre-cloacal supplements. Bendiella gen. nov. is most similar to Halichoanolaimus, but differs from the latter, and all other genera of the family Selachinematidae, in having a cuticle with lateral differentiation consisting of longitudinal rows of larger dots, and from all other genera of the Choniolaiminae in lacking pre-cloacal supplements. Bendiella thalassa gen. et sp. nov. is characterised by amphideal fovea with 5.25 turns, anterior buccal cavity with twelve rhabdions, each with a pair of pointed projections at posterior extremity, posterior buccal cavity with three Y-shaped pairs of slender rhabdions fused from two thirds of distance from anterior ends, and conico-cylindrical tail.

  2. Identification and phylogenetic analysis of Dirofilaria ursi (Nematoda: Filarioidea) from Wisconsin black bears (Ursus americanus) and its Wolbachia endosymbiont.

    Science.gov (United States)

    Michalski, Michelle L; Bain, Odile; Fischer, Kerstin; Fischer, Peter U; Kumar, Sanjay; Foster, Jeremy M

    2010-04-01

    Dirofilaria ursi is a filarial nematode of American black bears (Ursus americanus Pallas, 1780) that is vectored by black flies (Simuliidae) in many parts of the United States. In northwestern Wisconsin, the prevalence of microfilaremic bears during the fall hunting season was 21% (n = 47). Unsheathed blood microfilariae from Wisconsin bears possess characters consistent with the original description of D. ursi, as do adult worms observed histologically and grossly. Immunohistochemistry was used to identify the Wolbachia endosymbiont in the hypodermis and lateral cords of an adult female D. ursi. Amplification of wsp, gatB, coxA, fbpA, and ftsZ bacterial sequences from parasite DNA confirmed the presence of Wolbachia, and molecular phylogenetic analysis of the Wolbachia ftsZ gene groups the endosymbiont with Wolbachia from D. immitis and D. repens. Phylogenetic analysis of D. ursi 5s rDNA sequence confirms the morphological observations grouping this parasite as a member of Dirofilaria, and within the Dirofilaria - Onchocerca clade of filarial nematodes. This is the first report of Wolbachia characterization and molecular phylogeny information for D. ursi.

  3. Peculiarities of Embryonic and Post-Embryonic Development of Оesophagostomum dentatum (Nematoda, Strongylidae Larvae Cultured in Vitro

    Directory of Open Access Journals (Sweden)

    Yevstafieva V. А.

    2017-02-01

    Full Text Available Morphometric peculiarities of the development of Оesophagostomum dentatum Rudolphi, 1803 from egg to infective larva were studied under laboratory conditions at various temperatures. The determined optimum temperature for embryonic and post-embryonic development of О. dentatum larvae from domestic pig (Sus scrofa domesticus Linnaeus, 1758 is 22 °С. At this temperature, 81 % of larvae develop to the third stage (L3 on the 10th day. Temperatures of 24 °С and 20 °С are less favorable for the development of the nematode, at those temperatures only 67 and 63 % of larvae, respectively, reached infective stage by the 10th day of cultivation. Embryonic development of О. dentatum eggs is characterized by their lengthening (by 8.87-9.50 %, р < 0.01 and widening (by 6.77-9.35 %, р < 0.05-0.01, and post-embryonic larval development is associated with lengthening (by 4.59-17.33 %, р < 0.01-0.001.

  4. Pengendalian Nematoda Parasitik Tanaman secara Hayati dengan Bakteri Pasteuria penetrans: Inventarisasi, Pembiakan Massal, dan Uji Patogenisitas Isolat Bakteri

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    Mulyadi Mulyadi

    1995-12-01

    Full Text Available The research on “Biological control of plant parasitic nematodes by nematophagous bacteria (Pasteuria penetrans” was planned to be conducted within 3 years period, started in 1994/1995. In the first year research was done with the following objectives: 1 inventory on the isolates of P. penetrans, and 2 study on the mass production and pathogenicity test of P. penetrans. Survey of P. penetrans was done in the provinces of D.I. Yogyakarta, Central Java, East Java, and West Java. Soil and root samples were collected in this survey, plant parasitic nematodes were isolated using centrifugation and funnel and spray method. Mass production of P. penetrans was done with their host especially root-knot nematodes (Meloidogyne spp.. The bacterial infected nematodes were inoculated on tomato  planted on sterilized soil. Roots contain root-knot nematodes inhabiting spores of P. penetrans were used as inoculum source. The pathogenicity tests of the isolates were done using bioassay method and in the green house in Completely Randomized Design. The research results is as follows: 1 seventeesn isolates of P. penetrans were found in the survey, these isolates were pathogenic to root-knot nematodes, 2 mass production of P. penetrans was done with their host especially root-knot nematodes and 4 treatment with P. penetrans significantly reduced the root-gall caused by root-knot nematodes. Key words: biological control, parasitic nematodes, Pasteuria penetrans

  5. Two new gonad-infecting Philometra species (Nematoda: Philometridae) from the marine fish Lutjanus vitta (Perciformes: Lutjanidae) off New Caledonia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.

    2011-01-01

    Roč. 58, č. 4 (2011), s. 302-310 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : parasitic nematode * Philometra * MARINE FISH * Lutjanus * New Caledonia * South Pacific Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.812, year: 2011 http://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=22005

  6. Raphidascaris (Ichthyascaris) etelidis n. sp (Nematoda, Anisakidae), a new ascaridoid nematode from lutjanid fishes off New Caledonia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Justine, J.-L.

    2012-01-01

    Roč. 34, č. 1 (2012), s. 113-121 ISSN 1280-9551 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Parasitic nematode * Raphidascaris * Ichthyascaris * Etelis * Pristipomoides * marine fishes * New Caledonia * South Pacific * new species Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.696, year: 2012

  7. First Record of Anisakis simplex Third-Stage Larvae (Nematoda, Anisakidae in European Hake Merluccius merluccius lessepsianus in Egyptian Water

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    Yasmin Abou-Rahma

    2016-01-01

    Full Text Available The prevalence of infection and the identification of anisakid larvae in European hake Merluccius merluccius lessepsianus from Hurghada City, Red Sea Governorate, Egypt, were investigated. Fish samples were collected during the period of February and November 2014. Twenty-two (36.66% out of sixty examined fish specimens were found to be naturally infected with Anisakis type I larvae mostly found as encapsulated larvae in visceral organs. There was a positive relationship between host length/weight and prevalence of infection. Based on morphological, morphometric, and molecular analyses, these nematodes were identified as third-stage larvae of Anisakis simplex. The present study was considered as the first report of anisakid larvae from European hake in the Egyptian water.

  8. First Record of Anisakis simplex Third-Stage Larvae (Nematoda, Anisakidae) in European Hake Merluccius merluccius lessepsianus in Egyptian Water

    Science.gov (United States)

    Abou-Rahma, Yasmin; Abdel-Gaber, Rewaida; Kamal Ahmed, Amira

    2016-01-01

    The prevalence of infection and the identification of anisakid larvae in European hake Merluccius merluccius lessepsianus from Hurghada City, Red Sea Governorate, Egypt, were investigated. Fish samples were collected during the period of February and November 2014. Twenty-two (36.66%) out of sixty examined fish specimens were found to be naturally infected with Anisakis type I larvae mostly found as encapsulated larvae in visceral organs. There was a positive relationship between host length/weight and prevalence of infection. Based on morphological, morphometric, and molecular analyses, these nematodes were identified as third-stage larvae of Anisakis simplex. The present study was considered as the first report of anisakid larvae from European hake in the Egyptian water. PMID:26925257

  9. The occurrence of three species of the genus Oscheius Andrássy, 1976 (Nematoda: Rhabditida in Iran

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    Valizadeh Ahmad

    2017-09-01

    Full Text Available Due to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genus Oscheius were recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namely O. necromenus and O. onirici. Molecular data were also provided for a recently recovered isolate of O. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged to O. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations of O. onirici and O. tipulae forming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP, and presently sequenced isolates of O. tipulae and O. necromenus form a clade with other isolates of these species in 28S tree. The two species O. onirici and O. necromenus were reported in Iran for the first time.

  10. KAJIAN TINGKAT INFEKSI NEMATODA USUS PADA MURID SDN NAGARI LIMAU GADANG KABUPATEN PESISIR SELATAN PROVINSI SUMATERA BARAT

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    Armen Armen

    2016-09-01

    Full Text Available The research was done in Nagari Limau Gadang, West Sumatera. The purpose of the research was to study the rate of intestinal nematode infection on the students of SDN Nagari Limau Gadang.The population of the research was the students of SDN Nagari Limau Gadang as many as 100 students divided into six grades, that is grade 1 until grade 6. By using stratified proportional random sampling, 40% of the students on each grade were considered as the sample. The hypothesis of the research was 85% of the students were infected by intestinal nematode and 15% were not. As the result, the hypothesis was accepted; more than 75% of the students of SDN Nagari Limau Gadang in Pesisir Selatan regency were infected by intestinal nematode, especially Ascaris lumbricoides35% and Ancylostoma 8,33%. It was found that intestinal nematode mostly infected female students, the students under 9 years old, and those with uneducated parents.   Key words: infection, nematode, student

  11. Report of epibiont Thecacineta calix (Ciliophora: Suctorea) on deep sea Desmodora (Nematoda) from the Andaman Sea, Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Ingole, B.S.; Singh, R.; Sautya, S.; Dovgal, I.; Chatterjee, T.

    samples taken from the upper slope of the Northern Seamount located off Nicobar Island, 9859 0 31.52 00 N93857 0 15.48 00 E, from a water depth of 1301 m. Upon collection, the sponges were carefully separated from the sediment and immediately preserved... with absolute alcohol. In the laboratory, the sponge samples were washed carefully and the entire faunal community associated with sponge spicules were sorted out carefully and identified to the lowest possible taxa. All the nematodes were separated and fixed...

  12. Comparative studies on intestine ultrastructure of third-stage larvae and adults of Cystidicoloides ephemeridarum (Nematoda, Cystidicolidae).

    Science.gov (United States)

    Frantová, Denisa; Moravec, Frantisek

    2004-11-01

    The intestinal epithelium of third-stage larvae and adults of Cystidicoloides ephemeridarum from haemocoel of mayflies and stomach of brown trout was studied by electron microscopy and cytochemistry. In section, the intestine of both stages is composed of a single layer of about ten undifferentiated intestinal cells in a ring. A labyrinth of deep invaginations is present in the basal region of each cell. The apical surface is modified into well developed, regularly arranged microvilli. These, together with numerous organelles engaged in metabolism and a well defined gut lumen filled with unidentifiable material suggest that the intestine may function in digestion and absorption during both stages. The adults seem to feed upon the semifluid content of the stomach of brown trout. Fortuitous oral infection with undetermined bacteria in vitro led to degenerative changes in the intestinal tissue and probably caused death of the infected specimens. Up to 75% of the cell volume in the L(3) is occupied by glycogen deposits. In the adults, a minor portion of glycogen, together with lipid droplets, has been observed. The adults are considered to rely more on aerobic metabolism, whereas anaerobic metabolism (glycolysis) may prevail in L(3).

  13. Biochemical profile of Achatina fulica (Mollusca: Gastropoda) after infection by different parasitic loads of Angiostrongylus cantonensis (Nematoda, Metastrongylidae).

    Science.gov (United States)

    Tunholi-Alves, Vinícius Menezes; Tunholi, Victor Menezes; Amaral, Ludimila Santos; Mota, Esther Maria; Maldonado Júnior, Arnaldo; Pinheiro, Jairo; Garcia, Juberlan

    2015-01-01

    The effect of experimental infection by different parasitic loads of Angiostrongylus cantonensis (Nematode, Metastrongylidae) on the activities of the aminotransferases and concentration of total proteins, uric acid and urea in the hemolymph of Achatina fulica (Mollusca, Gastropoda) were investigated. There was a significant decrease in the concentration of total proteins in the exposed snails to 5000 or more larvae. This change was accompanied by an increase in the concentrations of urea and uric acid in the hemolymph, suggesting a higher rate of deamination of the amino acids. Besides this, variations in the activities of the aminotransferases were also observed, with the highest values recorded in the groups exposed to greater parasite load. These results suggest an increase in the use of total proteins, since there was increased formation of nitrogenous catabolites, in conformity with an increase in the aminotransferase activities. Infection was verified by the fact that L3 larvae recovered from the snails was proportion to the exposure dose of L1 larvae. Histopathological results also indicated presence of an inflammatory cell infiltrate, favoring an increase of both transaminases. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae employing mitochondrial COI gene sequence data

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    Monte Tainá CC

    2012-11-01

    Full Text Available Abstract Background The rat lungworm Angiostrongylus cantonensis can cause eosinophilic meningoencephalitis in humans. This nematode’s main definitive hosts are rodents and its intermediate hosts are snails. This parasite was first described in China and currently is dispersed across several Pacific islands, Asia, Australia, Africa, some Caribbean islands and most recently in the Americas. Here, we report the genetic variability among A. cantonensis isolates from different geographical locations in Brazil using mitochondrial cytochrome c oxidase subunit I (COI gene sequences. Methods The isolates of A. cantonensis were obtained from distinct geographical locations of Brazil. Genomic DNAs were extracted, amplified by polymerase reaction, purified and sequenced. A partial sequence of COI gene was determined to assess their phylogenetic relationship. Results The sequences of A. cantonensis were monophyletic. We identified a distinct clade that included all isolates of A. cantonensis from Brazil and Asia based on eight distinct haplotypes (ac1, ac2, ac3, ac4, ac5, ac6, ac7 and ac8 from a previous study. Interestingly, the Brazilian haplotype ac5 is clustered with isolates from Japan, and the Brazilian haplotype ac8 from Rio de Janeiro, São Paulo, Pará and Pernambuco states formed a distinct clade. There is a divergent Brazilian haplotype, which we named ac9, closely related to Chinese haplotype ac6 and Japanese haplotype ac7. Conclusion The genetic variation observed among Brazilian isolates supports the hypothesis that the appearance of A. cantonensis in Brazil is likely a result of multiple introductions of parasite-carrying rats, transported on ships due to active commerce with Africa and Asia during the European colonization period. The rapid spread of the intermediate host, Achatina fulica, also seems to have contributed to the dispersion of this parasite and the infection of the definitive host in different Brazilian regions.

  15. First report of Angiostrongylus cantonensis (Nematoda: Metastrongylidae in Achatina fulica (Mollusca: Gastropoda from Southeast and South Brazil

    Directory of Open Access Journals (Sweden)

    Arnaldo Maldonado Júnior

    2010-11-01

    Full Text Available The rat lungworm Angiostrongylus cantonensis is a worldwide-distributed zoonotic nematode that can cause human eosinophilic meningoencephalitis. Here, for the first time, we report the isolation of A. cantonensis from Achatina fulica from two Brazilian states: Rio de Janeiro (specifically the municipalities of Barra do Piraí, situated at the Paraiba River Valley region and São Gonçalo, situated at the edge of Guanabara Bay and Santa Catarina (in municipality of Joinville. The lungworms were identified by comparing morphological and morphometrical data obtained from adult worms to values obtained from experimental infections of A. cantonensis from Pernambuco, Brazil, and Akita, Japan. Only a few minor morphological differences that were determined to represent intra-specific variation were observed. This report of A. cantonensis in South and Southeast Brazil, together with the recent report of the zoonosis and parasite-infected molluscs in Northeast Brazil, provide evidence of the wide distribution of A. cantonensis in the country. The need for efforts to better understand the role of A. fulica in the transmission of meningoencephalitis in Brazil and the surveillance of molluscs and rodents, particularly in ports, is emphasized.

  16. Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) employing mitochondrial COI gene sequence data

    Science.gov (United States)

    2012-01-01

    Background The rat lungworm Angiostrongylus cantonensis can cause eosinophilic meningoencephalitis in humans. This nematode’s main definitive hosts are rodents and its intermediate hosts are snails. This parasite was first described in China and currently is dispersed across several Pacific islands, Asia, Australia, Africa, some Caribbean islands and most recently in the Americas. Here, we report the genetic variability among A. cantonensis isolates from different geographical locations in Brazil using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. Methods The isolates of A. cantonensis were obtained from distinct geographical locations of Brazil. Genomic DNAs were extracted, amplified by polymerase reaction, purified and sequenced. A partial sequence of COI gene was determined to assess their phylogenetic relationship. Results The sequences of A. cantonensis were monophyletic. We identified a distinct clade that included all isolates of A. cantonensis from Brazil and Asia based on eight distinct haplotypes (ac1, ac2, ac3, ac4, ac5, ac6, ac7 and ac8) from a previous study. Interestingly, the Brazilian haplotype ac5 is clustered with isolates from Japan, and the Brazilian haplotype ac8 from Rio de Janeiro, São Paulo, Pará and Pernambuco states formed a distinct clade. There is a divergent Brazilian haplotype, which we named ac9, closely related to Chinese haplotype ac6 and Japanese haplotype ac7. Conclusion The genetic variation observed among Brazilian isolates supports the hypothesis that the appearance of A. cantonensis in Brazil is likely a result of multiple introductions of parasite-carrying rats, transported on ships due to active commerce with Africa and Asia during the European colonization period. The rapid spread of the intermediate host, Achatina fulica, also seems to have contributed to the dispersion of this parasite and the infection of the definitive host in different Brazilian regions. PMID:23130987

  17. First report of Angiostrongylus cantonensis (Nematoda: Metastrongylidae) in Achatina fulica (Mollusca: Gastropoda) from Southeast and South Brazil.

    Science.gov (United States)

    Maldonado, Arnaldo; Simões, Raquel O; Oliveira, Ana Paula M; Motta, Esther M; Fernandez, Mônica A; Pereira, Zilene M; Monteiro, Simone S; Torres, Eduardo J Lopes; Thiengo, Silvana Carvalho

    2010-11-01

    The rat lungworm Angiostrongylus cantonensis is a worldwide-distributed zoonotic nematode that can cause human eosinophilic meningoencephalitis. Here, for the first time, we report the isolation of A. cantonensis from Achatina fulica from two Brazilian states: Rio de Janeiro (specifically the municipalities of Barra do Piraí, situated at the Paraiba River Valley region and São Gonçalo, situated at the edge of Guanabara Bay) and Santa Catarina (in municipality of Joinville). The lungworms were identified by comparing morphological and morphometrical data obtained from adult worms to values obtained from experimental infections of A. cantonensis from Pernambuco, Brazil, and Akita, Japan. Only a few minor morphological differences that were determined to represent intra-specific variation were observed. This report of A. cantonensis in South and Southeast Brazil, together with the recent report of the zoonosis and parasite-infected molluscs in Northeast Brazil, provide evidence of the wide distribution of A. cantonensis in the country. The need for efforts to better understand the role of A. fulica in the transmission of meningoencephalitis in Brazil and the surveillance of molluscs and rodents, particularly in ports, is emphasized.

  18. A new Huffmanela species, H. schouteni sp. n. (Nematoda: Trichosomoididae) from flying fishes in Curaçao.

    Science.gov (United States)

    Moravec, F; Campbell, B G

    1991-01-01

    A new nematode species, Huffmanela schouteni sp. n., has been established on the basis of its egg morphology and biological characters (adult nematodes are unknown). The dark-shelled eggs of this histozoic parasite occur in masses in the abdominal cavity, serose covers of internal organs and in the liver of the flying fishes Hirundichthys affinis Günther (type host) and Cypselurus cyanopterus Cuvier et Valenciennes in Curaçao. The eggs of H. schouteni sp. n. differ from those in other congeneric species mainly in the absence of small spines on the surface of the transparent envelope enclosing the egg proper, measurements (size of eggs 0.069-0.075 x 0.027-0.030 mm) and their localization in the host. A key to Huffmanela species based on egg morphology has been provided.

  19. Vittatidera zeaphila (Nematoda: Heteroderidae), a new genus and species of cyst nematode parasitic on corn (Zea mays).

    Science.gov (United States)

    Bernard, Ernest C; Handoo, Zafar A; Powers, Thomas O; Donald, Patricia A; Heinz, Robert D

    2010-06-01

    A new genus and species of cyst nematode, Vittatidera zeaphila, is described from Tennessee. The new genus is superficially similar to Cactodera but is distinguished from other cyst-forming taxa in having a persistent lateral field in females and cysts, persistent vulval lips covering a circumfenestrate vulva, and subventral gland nuclei of the female contained in a separate small lobe. Infective juveniles (J2) are distinguished from all previously described Cactodera spp. by the short stylet in the second-stage juvenile (14-17 μm); J2 of Cactodera spp. have stylets at least 18 μm long. The new species also is unusual in that the females produce large egg masses. Known hosts are corn and goosegrass. DNA analysis suggests that Vittatidera forms a separate group apart from other cyst-forming genera within Heteroderinae.

  20. Cactodera chenopodiae (Nematoda: Heteroderidae), a new species of cyst nematode parasitizing common lambsquarter (Chenopodium album) in Liaoning, China.

    Science.gov (United States)

    Feng, Yaxing; Wang, Dong; Xiao, Dongxue; Pereira, Tiago josÉ; Xuan, Yuanhu; Wang, Yuanyuan; Liu, Xiaoyu; Chen, Lijie; Duan, Yuxi; Zhu, Xiaofeng

    2018-04-11

    A new species of cyst nematode, Cactodera chenopodiae n. sp., parasitizing common lambsquarter, Chenopodium album L., is described from native vegetation in Liaoning, China. Cactodera chenopodiae n. sp. has a circumfenestrate pattern typical of the genus and is morphologically similar to C. cacti Krall Krall, 1978. However, in the new species, females and cysts show a larger L/W ratio whereas second-stage juveniles (J2s) have a longer hyaline region. The new species is also morphologically similar to C. milleri Graney Bird, 1990, but the J2s differ by a larger b ratio and longer tail. Based on DNA sequences of the 28S and ITS rRNA, C. chenopodiae n. sp. comes close to C. estonica Krall Krall, 1978, although it is distinct from the latter with respect to the presence of a punctate eggshell and larger b ratio in the J2s. Although morphometric comparisons with additional Cactodera species show the overlapping of diagnostic morphological characters, our phylogenetic analyses based on both rRNA genes support C. chenopodiae n. sp. as a unique lineage.

  1. Morphological and molecular genetic diversity of Strongyluris calotis (Nematoda: Ascaridida: Heterakidae) in South East and East Asian lizards.

    Science.gov (United States)

    Tran, Binh Thi; Ong, An Vinh; Luc, Pham Van; Sato, Hiroshi

    2016-07-01

    Strongyluris calotis is a heterakid nematode in the large intestine of agamid lizards (Reptilia: Sauria: Agamidae) from the Oriental Region. The standard light microscopic definition of the species counts the "caudal papillae" as 10 pairs on male worms. However, previous work from our group using scanning electron microscopy (SEM) on the heterakid from agamid lizards in Japan, Taiwan, and Singapore revealed that this counting contained a pair of phasmids and that two pairs of postcloacal papillae were completely fused to form a pair of united papillae, thus resulting in "10 pairs." In the present study, we examined S. calotis specimens from the Emma Gray's forest lizard, Calotes emma (Agamidae), living in the plain forest at low altitude, and the Vietnam false bloodsucker, Pseudocalotes brevipes (Agamidae), living in the mountainous forest at high altitude in the northern part of Vietnam. Using SEM, the arrangement of caudal papillae in male worms from an Emma Gray's forest lizard was found to be comparable to classical S. calotis specimens from agamid lizards collected in Japan, Taiwan, and Singapore. However, male worms from Vietnam false bloodsuckers did not have a pair of united papillae but had 10 pairs of independent caudal papillae with a pair of phasmids. Molecular genetic analyses of the ribosomal RNA gene (rDNA) of worms of the classical S. calotis morphotype from Japan and Singapore and two S. calotis morphotypes from Vietnam demonstrated absolutely identical nucleotide sequences of partial 18S rDNA (at least 1764 base pairs (bp)) and 5.8S rDNA (158 bp). However, intraspecific differences were detected in other regions of the rDNA, related to the geographical distribution of hosts regardless of morphotype: 97.8-98.5 % identity (443-446 bp/453 bp) in the internal transcribed spacer (ITS)-1 region, 96.6-98.0 % identity (425-431 bp/440 bp) in the ITS-2 region, and 99.6-99.7 % identity (1149-1151 bp/1154 bp) in the 28S rDNA. Thus, in the future, taxonomic relationships of S. calotis distributed widely in the Oriental Region as well as other nominal Oriental Strongyluris spp., currently six in number, need to be extensively explored based on molecular genetic analyses in addition to intensive morphological characterization.

  2. Pseudocapillaria (Ichthyocapillaria maricaensis n. sp. (Nematoda, Capillariidae and remarks on the helminthological fauna of Liolaemus lutzae Mertens, 1938 (Lacertilia, Iguanidae

    Directory of Open Access Journals (Sweden)

    H. de Oliveira Rodrigues

    1992-06-01

    Full Text Available Pseudocapillaria (Ichthyicapillaria maricaensis n. sp. is described from the small intestine of the lizard, Liolaemus lutzae Meterns, 1938, collected in the State of Rio de Janeiro Brazil. The author compares the new species with Capillaria crotaliRudolphi, 1819 Travassos, 1915, Capillaria freitaslenti Araujo & Gandra, 1941, Pseudocapillaria (Pseudocapillaria amarali (Freitas & Lent, 1934 Moravec, 1952, Pseudocapillaria (Pseudocapillaria cezarpintoi (Freitas & Lent, 1934Moravec, 1952 and Pseudocapillaria (Ichthyocapillaria murinae (travassos, 1914 Moravec, 1952 previously reported from lizards in Brazil. The nematode Thelandros sceleratus Travassos, 1923 and the trematode paradistomum parvissimum (Travassos, 1918 Travassos, 1919 are for the first time reported from this same host.

  3. Chimpanzee pinworm, Enterobius anthropopitheci (Nematoda: Oxyuridae), maintained for more than twenty years in captive chimpanzees in Japan.

    Science.gov (United States)

    Hasegawa, Hideo; Udono, Toshifumi

    2007-08-01

    The chimpanzee pinworm, Enterobius anthropopitheci (Gedoelst, 1916), was found in chimpanzees, Pan troglodytes, reared in Kumamoto Primate Research Park, Sanwa Kagaku Kenkyusho Co., Ltd., Kumamoto, Japan, in 2006. Because the chimpanzees in this institution originated from chimpanzees imported from Africa before 1984, it is considered that E. anthropopitheci infection has persisted for more than 20 yr in the chimpanzees. Analysis of pinworm specimens preserved in the institution revealed that transition of predominant pinworm species occurred, responding to the change of anthelmintics used for pinworm treatment. Present dominance of E. anthropopitheci is surmised to be caused by fenbendazole, which has been adopted from 2002. Scarcity of mixed infection with E. anthropopitheci and Enterobius vermicularis suggests interspecific competition between the pinworms.

  4. Morphology of chimpanzee pinworms, Enterobius (Enterobius) anthropopitheci (Gedoelst, 1916) (Nematoda: Oxyuridae), collected from chimpanzees, Pan troglodytes, on Rubondo island, Tanzania

    Czech Academy of Sciences Publication Activity Database

    Hasegawa, H.; Ikeda, Y.; Fujisaki, A.; Moscovice, L. R.; Petrželková, Klára Judita; Kaur, T.; Huffman, M. A.

    2005-01-01

    Roč. 91, č. 6 (2005), s. 1314-1317 ISSN 0022-3395 Grant - others:National Science Foundation(JP) 0238069; Japan Society for the Promotion of Science(JP) 12640680; GA AV ČR(CZ) AV0Z6093917-I028 Institutional research plan: CEZ:AV0Z60930519 Keywords : Pan troglodytes * pinworm s Subject RIV: EG - Zoology Impact factor: 1.524, year: 2005

  5. Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation.

    Science.gov (United States)

    Johnigk, S-A; Ecke, F; Poehling, M; Ehlers, R-U

    2004-06-01

    Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values <0.8 and the pH reached a maximum. The highest DJ recovery and yields were observed in cultures that were inoculated during the late stationary growth phase. This period started with the increase of the pH after its distinct minimum at pH <8.0. Thus optimal timing for DJ inoculation can be defined through monitoring of the pH in the P. luminescens culture.

  6. Pancreatitis associated with the helminth Serpinema microcephalus (Nematoda: Camallanidae) in exotic red-eared slider turtles (Trachemys scripta elegans).

    Science.gov (United States)

    Hidalgo-Vila, Judit; Martínez-Silvestre, Albert; Ribas, Alexis; Casanova, Joan Carles; Pérez-Santigosa, Natividad; Díaz-Paniagua, Carmen

    2011-01-01

    Pancreatitis associated with the helminth Serpinema microcephalus was found in three of 19 free-ranging red-eared slider turtles (Trachemys scripta elegans) captured between March 2003 and September 2004 in southern Spain. Microscopic changes were associated with parasite migrations and were characterized by central areas of necrosis surrounded by leukocytes and resulted in destruction of exocrine tissue. The blood profile of one of the three female turtles revealed eosinophilia and hyperglycemia, common in helminth infections and pancreatic disorders respectively. These are the first reported cases of pancreatitis caused by the nematode S. microcephalus in the exotic and newly colonized host T. s. elegans.

  7. Pancreatitis associated with the helminth serpinema microcephalus (Nematoda: Camallanidae) in exotic red-eared slider turtles (Trachemys scripta elegans)

    OpenAIRE

    Hidalgo-Vila, J.; Martínez-Silvestre, A.; Ribas, A.; Casanova, J.C.; Pérez-Santigosa, N.; Díaz-Paniagua, Carmen

    2011-01-01

    Pancreatitis associated with the helminth Serpinema microcephalus was found in three of 19 free-ranging red-eared slider turtles (Trachemys scripta elegans) captured between March 2003 and September 2004 in southern Spain. Microscopic changes were associated with parasite migrations and were characterized by central areas of necrosis surrounded by leukocytes and resulted in destruction of exocrine tissue. The blood profile of one of the three female turtles revealed eosinophilia and hyperglyc...

  8. Redescrição de Litomosoides brasiliensis Almeida, 1936 (Nematoda: Filariidae Parasito de Anoura caudifera (Chiroptera: Phyllostomidae

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    Mourão Elizabeth D

    2002-01-01

    Full Text Available The study of the surface topography added details regarding the disposition of male caudal papillae, spicules and area rugosa apart from vulva and oral aperture. The occurrence of this nematode in the state of Amapá represents a new geographical distribution.

  9. Two rare species of tylenchids, Discotylenchus biannulatus n. sp. and Labrys chinensis Qing Bert, 2018 (Nematoda: Tylenchidae) from western Iran.

    Science.gov (United States)

    Konani, Ehsan; Panahandeh, Yousef; Pourjam, Ebrahim; Álvarez-Ortega, Sergio; Pedram, Majid

    2018-04-23

    Two rare species of the family Tylenchidae are described and illustrated based on morphological and morphometric characters. Scanning electron microscope (SEM) studies provided details of anterior end structures, helpful in generic identification of the studied populations. Discotylenchus biannulatus n. sp. is characterized by its dorso-ventrally flattened smooth cephalic region having two proximal annuli and a rectangular perioral disc, short longitudinal amphidial slits, lateral field with four incisures, stylet 9-10 μm long, with the conus shorter than half the total stylet length and with posteriorly directed knobs, well-developed median bulb, mono-prodelphic reproductive system with rounded empty spermatheca and short postvulval uterine sac (PUS), and filiform tail with pointed end. It is compared with other species of Discotylenchus having four lines in the lateral field. The Iranian population of Labrys chinensis is characterized by its long and slender (a = 45.2-57.2) body, smooth rounded cephalic region and an offset disc-like apical labial plate, short longitudinal lateral amphidial slits, lateral field with two incisures, moderately developed stylet with the conus less than half the total length and posteriorly directed knobs, median bulb fusiform with distinct but weak valve, gradually joining the isthmus, vulva at 57.2-59.1% with small flaps, elongate conoid tail, uniformly and slightly narrowing toward end with broadly rounded terminus and rare males. The minor morphological differences of the recovered population with the type population are discussed.

  10. Phylogenetic relationship of the Brazilian isolates of the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) employing mitochondrial COI gene sequence data.

    Science.gov (United States)

    Monte, Tainá C C; Simões, Raquel O; Oliveira, Ana Paula M; Novaes, Clodoaldo F; Thiengo, Silvana C; Silva, Alexandre J; Estrela, Pedro C; Maldonado, Arnaldo

    2012-11-06

    The rat lungworm Angiostrongylus cantonensis can cause eosinophilic meningoencephalitis in humans. This nematode's main definitive hosts are rodents and its intermediate hosts are snails. This parasite was first described in China and currently is dispersed across several Pacific islands, Asia, Australia, Africa, some Caribbean islands and most recently in the Americas. Here, we report the genetic variability among A. cantonensis isolates from different geographical locations in Brazil using mitochondrial cytochrome c oxidase subunit I (COI) gene sequences. The isolates of A. cantonensis were obtained from distinct geographical locations of Brazil. Genomic DNAs were extracted, amplified by polymerase reaction, purified and sequenced. A partial sequence of COI gene was determined to assess their phylogenetic relationship. The sequences of A. cantonensis were monophyletic. We identified a distinct clade that included all isolates of A. cantonensis from Brazil and Asia based on eight distinct haplotypes (ac1, ac2, ac3, ac4, ac5, ac6, ac7 and ac8) from a previous study. Interestingly, the Brazilian haplotype ac5 is clustered with isolates from Japan, and the Brazilian haplotype ac8 from Rio de Janeiro, São Paulo, Pará and Pernambuco states formed a distinct clade. There is a divergent Brazilian haplotype, which we named ac9, closely related to Chinese haplotype ac6 and Japanese haplotype ac7. The genetic variation observed among Brazilian isolates supports the hypothesis that the appearance of A. cantonensis in Brazil is likely a result of multiple introductions of parasite-carrying rats, transported on ships due to active commerce with Africa and Asia during the European colonization period. The rapid spread of the intermediate host, Achatina fulica, also seems to have contributed to the dispersion of this parasite and the infection of the definitive host in different Brazilian regions.

  11. Microscopic and molecular characterization of Hepatozoon domerguei (Apicomplexa and Foleyella furcata (Nematoda in wild endemic reptiles from Madagascar

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    Maia João P.

    2014-01-01

    Full Text Available Madagascar is one of the world’s top twelve “megadiversity” hot spots hosting unique and threatened flora and fauna. Parasites are a major component of biodiversity but remain largely uncharacterized in wildlife. In this study we combine microscopic and molecular assessment of hemoparasites in endemic reptile species from Madagascar. We detected three distinct parasites: the apicomplexans Hepatozoon and Sarcocystis, and filarial nematodes. The prevalence and intensity of these apicomplexans were low overall, while microfilarial infections in chameleons were relatively high. We detected mixed infections of two Hepatozoon haplotypes in Madagascarophis colubrinus, and of Hepatozoon and microfilariae in a Furcifer sp. Phylogenetic analyses of Hepatozoon showed evidence of prey-predator transmission, with identical sequences found in the snakes M. colubrinus and Ithycyphus oursi, and their prey Furcifer sp. Based on previous studies regarding the life cycle of Hepatozoon domerguei Landau, Chabaud, Michel, and Brygoo, 1970 in these hosts and due to their morphological similarity, we propose that this Hepatozoon haplotype is Hepatozoon domerguei. Future studies, including the examination of invertebrate hosts, are needed to verify this preliminary taxonomic identification. A distinct hemogregarine haplotype was found in Oplurus sp., which displayed morphologically different gametocytes, some of which were apparently inside leukocytes. The Sarcocystis identified from Tracheloptychus petersi was identical to that reported in a North African snake, indicating that the same lineage is found in geographically distinct regions. By combining morphological and genetic information, Foleyella furcata (Linstow, 1899 filarial nematodes were identified in several Furcifer chameleons. This study provides insights into the distribution, diversity and host-parasite interactions of hemoparasites in wild reptile populations from Madagascar.

  12. Reappraisal of the specific status of Rhabdias (Nematoda: Rhabdiasoidea from Malagasy chameleons in the Paris Museum collection

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    Lhermitte-Vallarino N.

    2009-06-01

    Full Text Available To date Rhabdias gemellipara is the only species described from Malagasy chameleons, but heterogeneity of the material had been suspected. 11 samples of Rhabdias parasites present in the Paris Natural History Museum collection were examined. The size and shape of the buccal capsule, shape and length of the oesophagus, shape of the apical region of the intestine, extent of the genital tract and structure of the cuticular vesicle led to the distinction of five species. Rhabdias rabetafikae n. sp. parasitises Calumma cucullatum in the northeastern region (Cap Machoual. In the eastern region, R. nasutum n. sp. is parasitic in C. nasutum, and R. brevicorne n. sp. in C. brevicorne. All three species are similar in size to the African species parasitic in chameleons from which they can be distinguished by several characters. The small species, R. gemellipara, type host C. parsonii from the eastern region, was also found in C. brevicorne from the same geographic region. In the central region, Rhabdias sp., equally collected from C. brevicorne, is distinguished from R. gemellipara by a laterally flattened buccal capsule. All Malagasy species are hermaphrodites. Throughout the worm’s life spermatozoids are formed intermittently in a band of cells situated at 1-2 mm from the extremity of the ovaries; they migrate in the ovaries and accumulate in the oviducts.

  13. Morphological and biological study of Rhabdias spp. (Nematoda from african chameleons with description of a new species

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    Lhermitte-Vallarino N.

    2004-03-01

    Full Text Available Lung-dwelling females of Rhabdias (Rhabdiasidae, and possible migrating ceolomic young females were searched for in 46 chameleons, belonging to seven species. Rhabdias chamaeleonis, the single species identified to date in Africa, was found in Chamaeleo (Trioceros johnstoni and C. (T. hoehnelii, and redescribed ; the sizes of the buccal capsule and oesophagus were stable compared to the length of the female parasites, which varied from 6 mm to 22 mm in length. A second species, R. jarki n, sp., was identified from one C. (T. johnstoni ; it differed from R. chamaeleonis in the shape of anterior region, the longer and slender oesophagus (ratio bulb diameter-body diameter at that level about 1/5 instead of 1/2, the arrangement of the head papillae, the shape of the buccal capsule, and the anatomy of the genital apparatus : one of the ovaries (the anterior or posterior one according to the specimen had a band of small cells, among larger ovocytes of the synapsis zone, which were likely to generate the spermatozoa present in the oviducts, whereas these two elements were absent from R. chamaeleonis. The parasitic females of R. jarki thus appeared to be hermaphroditic, whereas those of R. chamaeleonis appeared to be parthenogenetic. The free living phase of these Rhabdias species was heterogonic. The infective larva of R. chamaeleonis was 360-590 µm long, unmotile and at third stage inside the maternal cuticle. The free-living male of R. jarki was described. The numerous infective larvae recovered from cultures of unidentified Rhabdias were all in maternal cuticle (one larva/female instead of two as in R. gemellipara from Calumma parsonii, from Madagascar and two kinds of larvae were identified, R. chamaeleonis and larvae 700-900 µm long provisionally identified to R. jarki. The behaviour of infective larvae and a few successful infections of insects suggest that, in the field, insect transport hosts are involved in transmission and in preventing infective larvae from drying out.

  14. Description of Uncinaria lyonsi n. sp. (Nematoda: Ancylostomatidae) from the California sea lion Zalophus californianus Lesson (Carnivora: Otariidae).

    Science.gov (United States)

    Kuzmina, Tetiana A; Kuzmin, Yuriy

    2015-02-01

    A new species of hookworm, Uncinaria lyonsi n. sp., is described based on morphological studies of the nematodes collected by Dr. E. T. Lyons from the California sea lion Zalophus californianus (Lesson) on San Miguel Island, California, USA. The new species is morphologically similar to three other species of the genus Uncinaria Frölich, 1789 parasitising pinnipeds, U. lucasi Stiles, 1901, U. hamiltoni Baylis, 1933 and U. sanguinis Marcus, Higgins, Šlapeta & Gray, 2014, in the body dimensions, the structure of the buccal capsule, the shape and structure of the male caudal bursa and female genital system. Uncinaria lyonsi n. sp. is differentiated from U. lucasi by having longer spicules and gubernaculum, larger buccal capsule and more slender oesophagus. The new species differs from U. hamiltoni and U. sanguinis in having shorter spicules and narrower buccal capsule. The latter two species also occur in the Southern Hemisphere and are geographically separated from U. lyonsi n. sp. The present study confirms the existence of a host-specific species of Uncinaria in the California sea lion, previously revealed by molecular and biological investigations.

  15. Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina

    Science.gov (United States)

    2013-01-01

    Background Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. Methods A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. Conclusions We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area. PMID:23988009

  16. Two gonad-infecting species of Philometra (Nematoda: Philometridae) from marine fishes off the northern coast of Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Barton, D.P.

    2015-01-01

    Roč. 22, FEB 6 2015 (2015), s. 4 ISSN 1252-607X R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Philometridae * new species * Australia Subject RIV: EG - Zoology Impact factor: 1.781, year: 2015

  17. Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina

    Czech Academy of Sciences Publication Activity Database

    Hernández-Orts, Jesús S.; Aznar, F. J.; Blasco-Costa, Maria Isabel; García, N.A.; Víllora-Montero, M.; Crespo, E.A.; Raga, J. A.; Montero, F. E.

    2013-01-01

    Roč. 6, AUG 2013 (2013), s. 252 ISSN 1756-3305 Institutional support: RVO:60077344 Keywords : Anisakidae * Eealworms * Pseudoterranova cattani * taxonomy * Ecology * Cox1 * Marine fishes * Southwestern Atlantic Subject RIV: EH - Ecology, Behaviour Impact factor: 3.251, year: 2013

  18. Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae).

    Science.gov (United States)

    Zhao, Wen-Ting; Guo, Yan-Ning; Zhang, Lu-Ping; Li, Liang

    2016-01-01

    Ascaridia galli (Schrank, 1788) is a common parasite of various galliform birds worldwide. Although A. galli has been extensively studied by many author, knowledge of the morphology of this species in detail is still insufficient. In the present paper, the detailed morphology of A. galli was further studied using light and scanning electron microscopy, based on specimens collected from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae) in China. The results revealed some erroneous and previously unreported morphological features, including the lips lacking real denticles, the lateral alae beginning at some distance posterior to the base of the ventrolateral lips and the caudal papillae with 4 different morphotypes. The present morphological and morphometric data complement previous descriptions and enable us to recognize this species more precisely.

  19. Structure of the female cephalic end and cuticular ornamentations of Paraphilometroides nemipteri (Nematoda: Philometridae), as revealed by SEM

    Czech Academy of Sciences Publication Activity Database

    Moravec, František

    2010-01-01

    Roč. 57, č. 4 (2010), s. 313-314 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Paraphilometroides * Nemipterus * Malaysia Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.533, year: 2010

  20. New species of Moravecia (Nematoda: Dracunculoidea) from body cavity of marine perciform fish Percophis brasiliensis in Argentina

    Czech Academy of Sciences Publication Activity Database

    Braicovich, P.; Moravec, František; Timi, J. A.

    2007-01-01

    Roč. 93, č. 2 (2007), s. 353-356 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Moravecia * Percophis * Argentina Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.129, year: 2007

  1. Mitochondrial genome of the eyeworm, Thelazia callipaeda (Nematoda: Spirurida, as the first representative from the family Thelaziidae.

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    Guo-Hua Liu

    Full Text Available Human thelaziosis is an underestimated parasitic disease caused by Thelazia species (Spirurida: Thelaziidae. The oriental eyeworm, Thelazia callipaeda, infects a range of mammalian definitive hosts, including canids, felids and humans. Although this zoonotic parasite is of socio-economic significance in Asian countries, its genetics, epidemiology and biology are poorly understood. Mitochondrial (mt DNA is known to provide useful genetic markers to underpin fundamental investigations, but no mt genome had been characterized for any members of the family Thelaziidae. In the present study, we sequenced and characterized the mt genome of T. callipaeda. This AT-rich (74.6% mt genome (13,668 bp is circular and contains 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lacks an atp8 gene. All protein-coding genes are transcribed in the same direction; the gene order is the same as those of Dirofilaria immitis and Setaria digitata (Onchocercidae, but distinct from Dracunculus medinensis (Dracunculidae and Heliconema longissimum (Physalopteridae. Phylogenetic analyses of the concatenated amino acid sequence data for all 12 protein-coding genes by Bayesian inference (BI showed that T. callipaeda (Thelaziidae is related to the family Onchocercidae. This is the first mt genome of any member of the family Thelaziidae and should represent a new source of genetic markers for studying the epidemiology, ecology, population genetics and systematics of this parasite of humans and other mammals.

  2. Camallanus emydidius n. sp. (Nematoda: Camallanidae) in Trachemys dorbigni (Duméril & Bibron, 1835) (Testudines: Emydidae) from Southern Brazil

    OpenAIRE

    Carolina S. Mascarenhas; Gertrud Müller

    2017-01-01

    This paper describes a new species of Camallanus found in the freshwater turtle Trachemys dorbigni. Sixty hosts collected in Southern Brazil were examined. All hosts (100%) were parasitized by a new species of Camallanus, which was described as Camallanus emydidius n. sp. The new species differs from other Camallanus species of freshwater turtles mainly because of the morphology of the right spicule, the number of male precloacal and postcloacal papillae, and the presence of ?mucrons? in the ...

  3. Camallanus emydidius n. sp. (Nematoda: Camallanidae) in Trachemys dorbigni (Duméril & Bibron, 1835) (Testudines: Emydidae) from Southern Brazil.

    Science.gov (United States)

    Mascarenhas, Carolina S; Müller, Gertrud

    2017-08-01

    This paper describes a new species of Camallanus found in the freshwater turtle Trachemys dorbigni. Sixty hosts collected in Southern Brazil were examined. All hosts (100%) were parasitized by a new species of Camallanus , which was described as Camallanus emydidius n. sp. The new species differs from other Camallanus species of freshwater turtles mainly because of the morphology of the right spicule, the number of male precloacal and postcloacal papillae, and the presence of "mucrons" in the female posterior extremity.

  4. Camallanus emydidius n. sp. (Nematoda: Camallanidae in Trachemys dorbigni (Duméril & Bibron, 1835 (Testudines: Emydidae from Southern Brazil

    Directory of Open Access Journals (Sweden)

    Carolina S. Mascarenhas

    2017-08-01

    Full Text Available This paper describes a new species of Camallanus found in the freshwater turtle Trachemys dorbigni. Sixty hosts collected in Southern Brazil were examined. All hosts (100% were parasitized by a new species of Camallanus, which was described as Camallanus emydidius n. sp. The new species differs from other Camallanus species of freshwater turtles mainly because of the morphology of the right spicule, the number of male precloacal and postcloacal papillae, and the presence of “mucrons” in the female posterior extremity.

  5. Molecular characterization and species delimiting of plant-parasitic nematodes of the genus Pratylenchus from the penetrans group (Nematoda: Pratylenchidae).

    Science.gov (United States)

    Janssen, Toon; Karssen, Gerrit; Orlando, Valeria; Subbotin, Sergei A; Bert, Wim

    2017-12-01

    Root-lesion nematodes of the genus Pratylenchus are an important pest parasitizing a wide range of vascular plants including several economically important crops. However, morphological diagnosis of the more than 100 species is problematic due to the low number of diagnostic features, high morphological plasticity and incomplete taxonomic descriptions. In order to employ barcoding based diagnostics, a link between morphology and species specific sequences has to be established. In this study, we reconstructed a multi-gene phylogeny of the Penetrans group using nuclear ribosomal and mitochondrial gene sequences. A combination of this phylogenetic framework with molecular species delineation analysis, population genetics, morphometric information and sequences from type location material allowed us to establish the species boundaries within the Penetrans group and as such clarify long-standing controversies about the taxonomic status of P. penetrans, P. fallax and P. convallariae. Our study also reveals a remarkable amount of cryptic biodiversity within the genus Pratylenchus confirming that identification on morphology alone can be inconclusive in this taxonomically confusing genus. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Species discovery and diversity in Lobocriconema (Criconematidae: Nematoda) and related plant-parasitic nematodes from North American ecoregions.

    Science.gov (United States)

    Powers, T O; Bernard, E C; Harris, T; Higgins, R; Olson, M; Olson, S; Lodema, M; Matczyszyn, J; Mullin, P; Sutton, L; Powers, K S

    2016-03-03

    There are many nematode species that, following formal description, are seldom mentioned again in the scientific literature. Lobocriconema thornei and L. incrassatum are two such species, described from North American forests, respectively 37 and 49 years ago. In the course of a 3-year nematode biodiversity survey of North American ecoregions, specimens resembling Lobocriconema species appeared in soil samples from both grassland and forested sites. Using a combination of molecular and morphological analyses, together with a set of species delimitation approaches, we have expanded the known range of these species, added to the species descriptions, and discovered a related group of species that form a monophyletic group with the two described species. In this study, 148 specimens potentially belonging to the genus Lobocriconema were isolated from soil, individually measured, digitally imaged, and DNA barcoded using a 721 bp region of cytochrome oxidase subunit 1 (COI). One-third of the specimens were also analyzed using amplified DNA from the 3' region of the small subunit ribosomal RNA gene (18SrDNA) and the adjacent first internal transcribed spacer (ITS1). Eighteen mitochondrial haplotype groups, falling into four major clades, were identified by well-supported nodes in Bayesian and maximum likelihood trees and recognized as distinct lineages by species delimitation metrics. Discriminant function analysis of a set of morphological characters indicated that the major clades in the dataset possessed a strong morphological signal that decreased in comparisons of haplotype groups within clades. Evidence of biogeographic and phylogeographic patterns was apparent in the dataset. COI haplotype diversity was high in the southern Appalachian Mountains and Gulf Coast states and lessened in northern temperate forests. Lobocriconema distribution suggests the existence of phylogeographic patterns associated with recolonization of formerly glaciated regions by eastern deciduous forest, but definitive glacial refugia for this group of plant parasitic nematodes have yet to be identified. Unlike agricultural pest species of plant-parasitic nematodes, there is little evidence of long-distance dispersal in Lobocriconema as revealed by haplotype distribution. Most haplotype groups were characterized by low levels of intragroup genetic variation and large genetic distances between haplotype groups. The localization of nematode haplotypes together with their characteristic plant communities could provide insight into the historical formation of these belowground biotic communities.

  7. Influence of Xenorhabdus (Gamma-Proteobacteria: Enterobacteriaceae) symbionts on gonad postembryonic development in Steinernema (Nematoda: Steinernematidae) nematodes.

    Science.gov (United States)

    Roder, Alexandra C; Stock, S Patricia

    2018-03-01

    Steinernema nematodes and their Xenorhabdus partners form an obligate mutualistic association. This partnership is insecticidal to a wide range of insects. Steinernema rely on their Xenorhabdus partner to produce toxins inside the insect cadaver to liberate nutrients from the insect, as well as antimicrobials to sterilize the cadaver, thus creating a suitable environment for reproduction. In return, Steinernema vector their Xenorhabdus between insect hosts. Disruption of this partnership may affect the success of both partners. For instance, when Steinernema associates with non-cognate symbionts, their virulence and reproductive fitness are affected. In this study, we examined the effect of symbiotic (cognate and non-cognate) and non-symbiotic bacteria on maturation time, gonad postembryonic development, and sex ratio of first-generation Steinernema adults. Two Steinernema spp. were considered: S. feltiae SN and S. carpocapsae All. In vitro assays were carried out by pairing each nematode sp. with symbiotic (cognate and non-cognate) Xenorhabdus, and with non-symbiotic bacteria (Serratia proteamaculans). Additionally, for comparative purposes, we also considered adult nematodes reared in vivo in Galleria mellonella larvae to assess nematode development under natural conditions. Results from this study showed non-symbiotic Serratia proteamaculans did not support adult development of S. feltiae but it allowed development of S. carpocapsae adults. Sex ratio decreased from 2:1 to 1:1 (female: male) when S. carpocapsae adults were reared with the non-symbiotic S. proteamaculans. Cognate or non-cognate Xenorhabdus spp. and/or strains did not change the sex ratio of any of either Steinernema spp. tested. Morphometric analysis also revealed that bacterial conditions influenced adult size and gonad postembryonic development in both Steinernema species. Body size (length and width), and gonad length in both S. feltiae males and females, were significantly reduced when reared with a non-cognate Xenorhabdus species. In S. carpocapsae, males exhibited an enhanced body size (length and width) and gonad length when reared with a non-cognate X. nematophila strain. S. carpocapsae females also exhibited an enhanced gonad length when reared with a non-cognate X. nematophila strain. S. carpocapsae males and females were underdeveloped when reared with the non-symbiotic S. proteamaculans, and exhibited reduced body sizes and gonad lengths. We conclude that development of first-generation adults of both Steinernema spp. tested, in particular time to adult maturation as well as body and gonad size were directly influenced by the bacterial symbionts they were cultured with. However, response to the culture conditions was species specific. Published by Elsevier Inc.

  8. A new species of Spauligodon (Nematoda; Oxyuroidea; Pharyngodonidae) and other Nematodes in Cyrtodactylus epiroticus (Squamata; Gekkonidae) from Papua New Guinea.

    Science.gov (United States)

    Bursey, Charles R; Goldberg, Stephen R; Kraus, Fred

    2017-12-20

    Spauligodon papuensis sp. nov. from the large intestines of Cyrtodactylus epiroticus (Gekkonidae) from Papua New Guinea is described and illustrated. Spauligodon papuensis sp. nov. represents the 54th species assigned to the genus and the second species from the Oceanic Region. The new species is separated from congeners by the unique combination of aspinose filamentous tail and no spicule in the male; and spinose filamentous tail, fusiform, flanged eggs, and postbulbar vulva in the female. Four additional species of nematodes were also found in C. epiroticus: mature specimens of Cosmocerca zugi, Falcaustra papuensis, Physalopteroides milnensis and larvae of Abbreviata sp.

  9. A survey of the Cape Floristic Region of South Africa for the presence of cyst nematodes (Nematoda: Heteroderidae).

    Science.gov (United States)

    Knoetze, Rinus; Swart, Antoinette

    2014-12-09

    A survey was performed to detect the presence of cyst nematodes in the Cape Floristic Region of South Africa. Soil was collected in the rhizosphere of the dominant plant species within blocks of indigenous vegetation and cysts were extracted from them. A total of 81 blocks of indigenous vegetation were sampled as described. Cysts were detected in 7 of these samples, representing 6 different vegetation types. One set of primers was used to amplify the ITS regions from these cysts, including the 5.8S ribosomal gene, as well as short parts of the 18S and 28S ribosomal genes. ITS-rDNA sequences from the indigenous isolates were aligned with selected sequences of other species from the Heteroderidae. Phylogenetic analyses to resolve the relationships between indigenous isolates and selected representatives of the Heteroderidae were conducted using the Maximum Parsimony method. The consensus tree resulting from alignment of the circumfenestrate cysts revealed that isolates SK18, WK1 and WK26 are included in a clade of Globodera species that parasitise non-solanaceous plants, forming a monophyletic group with G. millefolii, G. artemisiae, and an unidentified Globodera sp. from Portugal. In a tree resulting from the alignment of the Heterodera spp., isolates OK14 and WK2 are included in the Afenestrata group, forming a monophyletic group with H. orientalis.This survey unearthed at least four potentially new species of cyst nematodes, which may prove invaluable for the study of the evolution and biogeography of the group.

  10. Trehalose metabolism genes render rice white tip nematode Aphelenchoides besseyi (Nematoda: Aphelenchoididae) resistant to an anaerobic environment

    Science.gov (United States)

    Chen, Qiaoli; Zhang, Ruizhi; Ling, Yaming

    2018-01-01

    ABSTRACT After experiencing anaerobic environments, Aphelenchoides besseyi will enter a state of suspended animation known as anoxybiosis, during which it may use trehalose as an energy supply to survive. To explore the function of trehalose metabolism, two trehalose-6-phosphate synthase (TPS) genes (Ab-tps1 and Ab-tps2) encoding enzymes catalysing trehalose synthesis, and three trehalase (TRE) genes (Ab-ntre1, Ab-ntre2 and Ab-atre) encoding enzymes catalysing the hydrolysis of trehalose, were identified and investigated. Ab-tps1 and Ab-tps2 were active during certain periods of anoxybiosis for A. besseyi, and Ab-tps2, Ab-ntre1, Ab-ntre2 and Ab-atre were active during certain periods of recovery. The results of RNA interference experiments suggested that TRE genes regulated each other and both TPS genes, while a single TPS gene only regulated the other TPS gene. However, two TPS genes together could regulate TRE genes, which indicated a feedback mechanism between these genes. All these genes also positively regulated the survival and resumption of active metabolism of the nematode. Genes functioning at re-aeration have a greater impact on nematode survival, suggesting that these genes could play roles in anoxybiosis regulation, but may function within restricted time frames. Changes in trehalose levels matched changes in TRE activity during the anoxybiosis–re-aeration process, suggesting that trehalose may act as an energy supply source. The observation of up-regulation of TPS genes during anoxybiosis suggested a possible signal role of trehalose. Trehalose metabolism genes could also work together to control trehalose levels at a certain level when the nematode is under anaerobic conditions. PMID:29158222

  11. Mitochondrial genomes of Meloidogyne chitwoodi and M. incognita (Nematoda: Tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.

    Science.gov (United States)

    Humphreys-Pereira, Danny A; Elling, Axel A

    2014-01-01

    Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respectively. The mt genomes each contain a large non-coding region with tandem repeats and the control region. The mt gene arrangement of M. chitwoodi and M. incognita is unlike that of other nematodes. Sequence alignments of the two Meloidogyne mt genomes showed three translocations; two in transfer RNAs and one in cox2. Compared with other nematode mt genomes, the gene arrangement of M. chitwoodi and M. incognita was most similar to Pratylenchus vulnus. Phylogenetic analyses (Maximum Likelihood and Bayesian inference) were conducted using 78 complete mt genomes of diverse nematode species. Analyses based on nucleotides and amino acids of the 12 protein-coding mt genes showed strong support for the monophyly of class Chromadorea, but only amino acid-based analyses supported the monophyly of class Enoplea. The suborder Spirurina was not monophyletic in any of the phylogenetic analyses, contradicting the Clade III model, which groups Ascaridomorpha, Spiruromorpha and Oxyuridomorpha based on the small subunit ribosomal RNA gene. Importantly, comparisons of mt gene arrangement and tree-based methods placed Meloidogyne as sister taxa of Pratylenchus, a migratory plant endoparasitic nematode, and not with the sedentary endoparasitic Heterodera. Thus, comparative analyses of mt genomes suggest that sedentary endoparasitism in Meloidogyne and Heterodera is based on convergent evolution. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. The complete mitochondrial genome of Setaria digitata (Nematoda: Filarioidea): Mitochondrial gene content, arrangement and composition compared with other nematodes.

    Science.gov (United States)

    Yatawara, Lalani; Wickramasinghe, Susiji; Rajapakse, R P V J; Agatsuma, Takeshi

    2010-09-01

    In the present study, we determined the complete mitochondrial (mt) genome sequence (13,839bp) of parasitic nematode Setaria digitata and its structure and organization compared with Onchocerca volvulus, Dirofilaria immitis and Brugia malayi. The mt genome of S. digitata is slightly larger than the mt genomes of other filarial nematodes. S. digitata mt genome contains 36 genes (12 protein-coding genes, 22 transfer RNAs and 2 ribosomal RNAs) that are typically found in metazoans. This genome contains a high A+T (75.1%) content and low G+C content (24.9%). The mt gene order for S. digitata is the same as those for O. volvulus, D. immitis and B. malayi but it is distinctly different from other nematodes compared. The start codons inferred in the mt genome of S. digitata are TTT, ATT, TTG, ATG, GTT and ATA. Interestingly, the initiation codon TTT is unique to S. digitata mt genome and four protein-coding genes use this codon as a translation initiation codon. Five protein-coding genes use TAG as a stop codon whereas three genes use TAA and four genes use T as a termination codon. Out of 64 possible codons, only 57 are used for mitochondrial protein-coding genes of S. digitata. T-rich codons such as TTT (18.9%), GTT (7.9%), TTG (7.8%), TAT (7%), ATT (5.7%), TCT (4.8%) and TTA (4.1%) are used more frequently. This pattern of codon usage reflects the strong bias for T in the mt genome of S. digitata. In conclusion, the present investigation provides new molecular data for future studies of the comparative mitochondrial genomics and systematic of parasitic nematodes of socio-economic importance. 2010 Elsevier B.V. All rights reserved.

  13. Four new species of free-living marine nematodes of the family Comesomatidae (Nematoda: Araeolaimida) from coast of Vietnam.

    Science.gov (United States)

    Gagarin, Vladimir G

    2013-01-24

    Specimens of four new nematode species of the family Comesomatidae were isolated from the sediments of littoral zone of South China Sea at the coast of Vietnam and described and illustrated. Sabatieria curvispiculata sp. n. is characterized by the long and slender tail, short cephalic setae and strongly curved spicules in males. Setosabatiera orientalis sp. n. is close to S. australis Riera, Nunez, Brito, 2006, but differs from it in the comparatively shorter and more slender tail, small-er number of amphidial fovea turns, greater number of precloacal supplements in males and shape and structure of spic-ules. Dorylaimopsis intermedia sp. n. is morphologically closest to D. mediterranea Grimaldi-de Zio, 1968 and D. magellanense Chen, Vincx, 1968, but differs from both species in the longer outer labial setae and absence of precloacal supplements in males. D. brevispiculata sp. n. is similar to D. turneri Zhang, 1992 and D. coomansi Muthumbi, Soetaert, Vincx, 1977, but differs from both species in the shape of outer labial sensillae and absence of precloacal supplements in males. A pictorial key for determination of valid species in the genus Setosabatieria Rouville, 1903 is given.

  14. Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (gamma-Proteobacteria: Enterobacteriaceae) in Jordan.

    Science.gov (United States)

    Stock, S Patricia; Al Banna, Luma; Darwish, Rula; Katbeh, Ahmad

    2008-06-01

    Until now, only a few systematic surveys of entomopathogenic nematodes (EPN) have been conducted in Middle Eastern countries. Many of the recovered EPN species in this region have shown to own distinctive qualities that enable their survival in unique environments, such as high temperatures and low moisture levels tolerance. These new species and strains, with unique environmental tolerances, are more suitable for their consideration in pest management programs in xerophytic regions. With this background in mind, we recently conducted a survey of EPN in Jordan. This study records for the first time the diversity and distribution of these nematodes and their bacterial symbionts in this country. Jordan's three geographic regions: (1) the highlands, (2) Jordan valley and (3) the desert region were sampled. Within each region, natural habitats and agricultural regions characteristic to each region were considered for sampling purposes. Four EPN species including three Steinernema and one Heterorhabditis were recovered. Nematodes were identified using a combination of molecular markers and classic morphological diagnostic tools. Bacterial symbionts were identified by analysis of 16S rRNA sequences. Abiotic characteristics such as soil type, soil pH, and elevation were also recorded. We herein report the diversity of EPN species in Jordan and discuss their potential in Biocontrol and IPM programs for this country.

  15. Cucullanid nematodes (Nematoda: Cucullanidae) from deep-sea marine fishes off New Caledonia, including Dichelyne etelidis n. sp.

    Science.gov (United States)

    Moravec, František; Justine, Jean-Lou

    2011-02-01

    Three nematode species of the family Cucullanidae, intestinal parasites of marine perciform fishes, are reported from off New Caledonia: Cucullanus bourdini Petter & Le Bel, 1992 from the crimson jobfish Pristipomoides filamentosus (Valenciennes) and the goldflag jobfish Pristipomoides auricilla (Jordan, Evermann & Tanaka) (new host record) (both Lutjanidae); Dichelyne etelidis n. sp. from the deep-water red snapper Etelis carbunculus Cuvier (type-host) and the deep-water longtail red snapper Etelis coruscans Valenciennes (both Lutjanidae); and Dichelyne sp. (only one female) from the trumpet emperor Lethrinus miniatus (Forster) (Lethrinidae). Detailed light and electron microscopical studies revealed in C. bourdini some taxonomically important, previously unreported features, such as the location of the excretory pore, nature of the vulva and the size of fully-developed eggs. The new species, D. etelidis, is characterised mainly by the length of the spicules (462-748 μm), a single intestinal caecum, the location of the deirids and excretory pore, the arrangement of the genital papillae and the host group.

  16. Population genetic structure in Sabatieria (Nematoda) reveals intermediary gene flow and admixture between distant cold seeps from the Mediterranean Sea.

    Science.gov (United States)

    De Groote, Annelies; Hauquier, Freija; Vanreusel, Ann; Derycke, Sofie

    2017-07-01

    There is a general lack of information on the dispersal and genetic structuring for populations of small-sized deep-water taxa, including free-living nematodes which inhabit and dominate the seafloor sediments. This is also true for unique and scattered deep-sea habitats such as cold seeps. Given the limited dispersal capacity of marine nematodes, genetic differentiation between such geographically isolated habitat patches is expected to be high. Against this background, we examined genetic variation in both mitochondrial (COI) and nuclear (18S and 28S ribosomal) DNA markers of 333 individuals of the genus Sabatieria, abundantly present in reduced cold-seep sediments. Samples originated from four Eastern Mediterranean cold seeps, separated by hundreds of kilometers, and one seep in the Southeast Atlantic. Individuals from the Mediterranean and Atlantic were divided into two separate but closely-related species clades. Within the Eastern Mediterranean, all specimens belonged to a single species, but with a strong population genetic structure (Φ ST  = 0.149). The haplotype network of COI contained 19 haplotypes with the most abundant haplotype (52% of the specimens) shared between all four seeps. The number of private haplotypes was high (15), but the number of mutations between haplotypes was low (1-8). These results indicate intermediary gene flow among the Mediterranean Sabatieria populations with no evidence of long-term barriers to gene flow. The presence of shared haplotypes and multiple admixture events indicate that Sabatieria populations from disjunct cold seeps are not completely isolated, with gene flow most likely facilitated through water current transportation of individuals and/or eggs. Genetic structure and molecular diversity indices are comparable to those of epiphytic shallow-water marine nematodes, while no evidence of sympatric cryptic species was found for the cold-seep Sabatieria.

  17. Two new species of nematodes (Nematoda) from highly mineralized rivers of Lake El'ton basin, Russia.

    Science.gov (United States)

    Gusakov, Vladimir A; Gagarin, Vladimir G

    2016-09-05

    Two new nematode species, Mesodorylaimus rivalis sp. n. and Allodiplogaster media sp. n., from the highly mineralized rivers of the El'ton Lake basin (Russia) are described and illustrated from numerous mature females and males. Mesodorylaimus rivalis sp. n. is similar to M. vulvapapillatus Bagaturia & Eliava, 1966, but differs from it in the longer body, shorter spicules and longer female prerectum. Allodiplogaster media sp. n. resembles A. lupata (Shoshin, 1989) Kanzaki, Ragsdale & Giblin-Davis, 2014 and A. mordax (Shoshin, 1989) Kanzaki, Ragsdale & Giblin-Davis, 2014, but differs from the first species in having a shorter pharynx, shorter outer labial setae, longer spicules and different ratio between anterior and posterior pharynx sections, and from A. mordax in the thinner body, shorter pharynx and longer spicules.

  18. Spray nozzles, pressures, additives and stirring time on viability and pathogenicity of entomopathogenic nematodes (nematoda: rhabditida) for greenhouses.

    Science.gov (United States)

    Moreira, Grazielle Furtado; Batista, Elder Simões de Paula; Campos, Henrique Borges Neves; Lemos, Raphael Emilio; Ferreira, Marcelo da Costa

    2013-01-01

    The objective of this study was to evaluate different strategies for the application of entomopathogenic nematodes (EPN). Three different models of spray nozzles with air induction (AI 11003, TTI 11003 and AD-IA 11004), three spray pressures (207, 413 and 720 kPa), four different additives for tank mixtures (cane molasses, mineral oil, vegetable oil and glycerin) and the influence of tank mixture stirring time were all evaluated for their effect on EPN (Steinernema feltiae) viability and pathogenicity. The different nozzles, at pressures of up to 620 kPa, were found to be compatible with S. feltiae. Vegetable oil, mineral oil and molasses were found to be compatible adjuvants for S. feltiae, and stirring in a motorized backpack sprayer for 30 minutes did not impact the viability or pathogenicity of this nematode. Appropriate techniques for the application of nematodes with backpack sprayers are discussed.

  19. Mansonella ozzardi (Nematoda: Onchocercidae in the riverine population of the Tefé River, State of Amazonia, Brazil

    Directory of Open Access Journals (Sweden)

    Jansen Fernandes Medeiros

    2014-01-01

    Full Text Available Introduction: This study assessed the prevalence of Mansonella ozzardi in riverine communities of the Tefé River, Amazonas, Brazil. Methods: The prevalence of M. ozzardi was estimated by microscopic examination of thick blood smears. Results: The M. ozzardi prevalence rate was 6.3% (19/300. Filarial infection was found in 8 of the 11 communities surveyed, with prevalence rates varying from 2.5% to 22.2%. Conclusions: Tefé is a region of oil and natural gas exploration, in which there is a high turnover of workers. Migration patterns may facilitate the dissemination of mansonelliasis to other regions.

  20. Comparative life cycles and life histories of North American Rhabdias spp. (Nematoda: Rhabdiasidae): lungworms from snakes and anurans.

    Science.gov (United States)

    Langford, Gabriel J; Janovy, John

    2009-10-01

    The present study used experimental infections to compare the life cycles and life histories of 6 Rhabdias spp. infecting snakes and anurans. Free-living development of anuran lungworms was primarily limited to heterogonic reproduction, and females utilized matricidal endotoky exclusively, whereas snake lungworms primarily reproduced homogonically and, when heterogonic reproduction occurred, females used a combination of releasing eggs and matricidal endotoky. Infective snake lungworms survived for longer periods in fresh water compared to anuran worms. Infective anuran lungworms penetrated into the skin of frogs and toads; few infections resulted from per os infections. In contrast, snake lungworms were unable to penetrate skin; instead, infective juveniles penetrated into snake esophageal tissue during per os infections. Despite separate points of entry, anuran and snake lungworms both migrated and developed in the fascia, eventually penetrating into the body cavity of the host. Worms molted to adulthood inside the body cavity and subsequently penetrated into the host's lungs, where they fed on blood while becoming gravid. Adult lungworm survival varied among lungworm species, but, in general, snake lungworms were longer lived than anuran worms. Anuran lungworms were poorly suited for transmission via transport hosts, whereas snake lungworms were consistently capable of establishing infections using transport hosts. Overall, these observations suggest that snake and anuran lungworms have discrepant life cycles and life history strategies.

  1. Capillostrongyloides morae sp. n. (Nematoda: Capillariidae) from deep-sea fish (Teleostei, Moridae) in the western Mediterranean Sea

    Czech Academy of Sciences Publication Activity Database

    González-Solís, David; Carrasson, M.; Pérez-del-Olmo, A.

    2014-01-01

    Roč. 61, č. 1 (2014), s. 63-68 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Gadiformes * Lepidion lepidion * Mora moro * Spain Subject RIV: EG - Zoology Impact factor: 1.147, year: 2014

  2. Tziminema unachi n. gen., n. sp. (Nematoda: Strongylidae: Strongylinae) parasite of Baird's tapir Tapirus bairdii from Mexico.

    Science.gov (United States)

    Güiris-Andrade, D M; Oceguera-Figueroa, A; Osorio-Sarabia, D; Pérez-Escobar, M E; Nieto-López, M G; Rojas-Hernández, N M; García-Prieto, L

    2017-11-20

    A new genus and species of nematode, Tziminema unachi n. gen., n. sp. is described from the caecum and colon of Baird's tapir Tapirus bairdii (Gill, 1865), found dead in the Reserva de la Biósfera El Triunfo, Chiapas State, in the Neotropical realm of Mexico. Tziminema n. gen. differs from the other nine genera included in the Strongylinae by two main characteristics: having 7-9 posteriorly directed tooth-like structures at the anterior end of the buccal capsule, and the external surface of the buccal capsule being heavily striated. Phylogenetic analyses of the DNA sequences of the mitochondrial cytochrome c oxidase and nuclear DNA, including a partial sequence of the internal transcribed spacer 1, 5.8S and a partial sequence of the internal transcribed spacer 2 of the new taxon, confirmed its inclusion in Strongylinae and its rank as a new genus.

  3. Effects of excretory/secretory products from Anisakis simplex (Nematoda) on immune gene expression in rainbow trout (Oncorhynchus mykiss)

    DEFF Research Database (Denmark)

    Bahlool, Qusay Zuhair Mohammad; Skovgaard, Alf; Kania, Per Walter

    2013-01-01

    Excretory/secretory (ES) products are molecules produced by parasitic nematodes, including larval Anisakis simplex, a parasite occurring in numerous marine fish hosts. The effects of these substances on host physiology have not been fully described. The present work elucidates the influence of ES...... substances on the fish immune system by measuring immune gene expression in spleen and liver of rainbow trout (Oncorhynchus mykiss) injected intraperitoneally with ES products isolated from A. simplex third stage larvae. The overall gene expression profile of exposed fish showed a generalized down....... This type of hydrolytic enzyme activity may play a role in nematode penetration of host tissue. In addition, based on the notion that A. simplex ES products may have an immune-depressive effect (by minimizing immune gene expression) it could also be suggested that worm enzymes directly target host immune...

  4. Larvae of Contracaecum sp. (Nematoda: Anisakidae) in the threatened freshwater fish Sandelia capensis (Anabantidae) in South Africa

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; van Rensburg, C.J.; Van As, L. L.

    2016-01-01

    Roč. 120, č. 3 (2016), s. 251-254 ISSN 0177-5103 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * third-stage larva * paratenic host * endangered fish species * Africa Subject RIV: EG - Zoology Impact factor: 1.549, year: 2016

  5. Characterisation of the vascular pathology in Sigmodon hispidus (Rodentia: Cricetidae following experimental infection with Angiostrongylus costaricensis (Nematoda: Metastrongylidae

    Directory of Open Access Journals (Sweden)

    Danielle Ingrid Bezerra de Vasconcelos

    Full Text Available BACKGROUND Angiostrongylus costaricensis is a nematode that causes human abdominal angiostrongyliasis, a disease found mainly in Latin American countries and particularly in Brazil and Costa Rica. Its life cycle involves exploitation of both invertebrate and vertebrate hosts. Its natural reservoir is a vertebrate host, the cotton rat Sigmodon hispidus. The adult worms live in the ileo-colic branches of the upper mesenteric artery of S. hispidus, causing periarteritis. However, there is a lack of data on the development of vasculitis in the course of infection. OBJECTIVE To describe the histopathology of vascular lesions in S. hispidus following infection with A. costaricensis. METHODS Twenty-one S. hispidus were euthanised at 30, 50, 90 and 114 days post-infection (dpi, and guts and mesentery (including the cecal artery were collected. Tissues were fixed in Carson’s Millonig formalin, histologically processed for paraffin embedding, sectioned with a rotary microtome, and stained with hematoxylin-eosin, resorcin-fuchsin, Perls, Sirius Red (pH = 10.2, Congo Red, and Azan trichrome for brightfield microscopy analysis. FINDINGS At 30 and 50 dpi, live eggs and larvae were present inside the vasa vasorum of the cecal artery, leading to eosinophil infiltrates throughout the vessel adventitia and promoting centripetal vasculitis with disruption of the elastic layers. Disease severity increased at 90 and 114 dpi, when many worms had died and the intensity of the vascular lesions was greatest, with intimal alterations, thrombus formation, iron accumulation, and atherosclerosis. CONCLUSION In addition to abdominal angiostrongyliasis, our data suggest that this model could be very useful for autoimune vasculitis and atherosclerosis studies.

  6. A new species of Philometra (Nematoda: Philometridae) from the sand perch Diplectrum formosum (Serranidae) off Florida, northern Gulf of Mexico

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Bakenhaster, M.

    2010-01-01

    Roč. 96, č. 5 (2010), s. 987-992 ISSN 0022-3395 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Diplectrum * Gulf of Mexico Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.208, year: 2010

  7. Anisakis simplex (Nematoda: Anisakidae) third-stage larval infections of marine cage cultured cobia, Rachycentron canadum L., in Taiwan.

    Science.gov (United States)

    Shih, Hsiu-Hui; Ku, Chen-Chun; Wang, Chun-Shun

    2010-08-04

    The first confirmed case of Anisakis simplex infection of the marine cage cobia, Rachycentron canadum (L.), was recorded in Taiwan. The case investigation revealed the presence of third-stage larvae (L3) in either the stomach lumen or abdominal cavity of the cobia but never within the musculatures. Larvae were mainly encapsulated in the peritoneal mesentery on the outer surface of the stomach wall and occasionally on the liver surface. Part of the diet fed to the cobia includes chopped raw fish, and of these, seven species were found to harbor these larvae (as paratenic hosts), indicating that these particular fish might be the larval sources for this infection. To illustrate the course of infection and distribution of this parasite inside cobia, both juvenile and adult cobia were experimentally infected with live L3 by oral transmission. The prevalence of infection reached 100% at the end of all trials. The course of the infection was assessed after necropsy by histological and ultrastructural observations. A. simplex L3 recovered from various locations within juvenile cobia at different post-infection (p.i.) times were at the L3 stage and did not grow significantly. The L3 either adhered to or penetrated into the gastric mucosa of cobia by 2 h p.i. By 25 d p.i., many were trapped within the submucosa and encapsulated by fibroconnective tissue. This phenomenon was more apparent in adult cobia, such that 37.5-86.0% of the injected L3 were primarily found encapsulated within the gastric submucosa. Based upon a PCR-RFLP assay, the larvae encountered in this study were identified as having a recombinant genotype of A. simplex sensu stricto and A. pegreffii. Based upon the results of this study, strategies to ensure the safety of seafood manufactured from cobia and to prevent the potential risks of anisakiasis or allergies risk to consumers were suggested. Copyright 2010 Elsevier B.V. All rights reserved.

  8. On the species status of the root-knot nematode Meloidogyne ulmi Palmisano & Ambrogioni, 2000 (Nematoda, Meloidogynidae

    Directory of Open Access Journals (Sweden)

    Mohammed Ahmed

    2013-12-01

    Full Text Available The root-knot nematode Meloidogyne ulmi is synonymised with Meloidogyne mali based on morphological and morphometric similarities, common hosts, as well as biochemical similarities at both protein and DNA levels. M. mali was first described in Japan on Malus prunifolia Borkh.; and M. ulmi in Italy on Ulmus chenmoui W.C. Cheng. Morphological and morphometric studies of their holo- and paratypes revealed important similarities in the major characters as well as some general variability in a few others. Host test also showed that besides the two species being able to parasitize the type hosts of the other, they share some other common hosts. Our study of the esterase and malate dehydrogenase isozyme phenotypes of some M. ulmi populations gave a perfectly comparable result to that already known for M. mali. Finally, phylogenetic studies of their SSU and LSU rDNA sequence data revealed that the two are not distinguishable at DNA level. All these put together, leave strong evidences to support the fact that M. ulmi is not a valid species, but a junior synonym of M. mali. Brief discussion on the biology and life cycle of M. mali is given. An overview of all known hosts and the possible distribution of M. mali in Europe are also presented.

  9. New data on the morphology and taxonomy of three species of Rhabdochona (Nematoda: Rhabdochonidae) parasitizing fishes in India

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Scholz, Tomáš; Ash, Anirban; Kar, P. K.

    2010-01-01

    Roč. 57, č. 4 (2010), s. 295-306 ISSN 0015-5683 R&D Projects: GA MŠk LC522; GA ČR GA524/08/0885; GA ČR GD206/09/H026 Institutional research plan: CEZ:AV0Z60220518 Keywords : parasitic nematode * Rhabdochona * morphology * taxonomy * freshwater fish * Schizothorax * Tor * Clupisoma * India Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.533, year: 2010

  10. Gonad-infecting species of Philometra (Nematoda: Philometridae) from groupers Epinephelus spp. (Osteichthyes: Serranidae) in the Bay of Bengal, India

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Manoharan, J.

    2014-01-01

    Roč. 59, č. 4 (2014), s. 596-605 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Parasitic nematode * new species * Dracunculoidea * marine fish * Indian Ocean Subject RIV: EG - Zoology Impact factor: 0.905, year: 2014

  11. Post-embryonic development of Camallanus cotti (Nematoda: Camallanidae), with emphasis on growth of some taxonomically important somatic characters.

    Science.gov (United States)

    Levsen, Arne; Berland, Bjørn

    2002-01-01

    In this paper, the quantitative post-embryonic development of the Asian freshwater fish nematode Camallanus cotti Fujita, 1927, is described. Larval and adult morphometrics were obtained by following the parasite's life cycle experimentally using copepods Macrocyclops albidus (Jurine) as intermediate host and guppies Poecilia reticulata (Peters), southern platyfish Xiphophorus maculatus (Günther) and paradise fish Macropodus opercularis (L.) as definitive host. Additionally, adult worms were obtained from heavily infected paradise fish imported from Singapore. It is suggested that the gradual change in proportions of the worm's somatic body parts reflects the specific ecological role of each developmental stage. The free-living infective first-stage larva seems to be adapted for transmission, as indicated by its relatively long tail, designed to generate host-attracting movements, and its non-functional intestine. The second- and third-stage larvae from the copepod intermediate host seem mainly to invest in trophic functionality, i.e., the development of the buccal capsule and the oesophagus, which are crucial structures for the worm's successful establishment in the definitive fish host. Once in the fish intestine, the larvae enter a period of considerable growth. After the fourth (i.e., last) moult, a 72% increase in average female body length occurs. This is accompanied by doubling the average vulva-tail tip distance and the average tail length. The length of the female hind body expands in an accelerating allometric fashion, and seems to be closely linked to the posterior-wards expansion of the uterus. In the males however, growth seems to cease after the final moult. We conclude that female post-maturational body size, but especially the length of the hind body and the tail, are closely related to reproductive state, i.e., the developmental stage of the offspring in the uterus, and, probably, the worms' age. Any future taxonomical studies of camallanids in general, and C. cotti in particular, should thus be aware of the reproductive state of the females used.

  12. Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina.

    Science.gov (United States)

    Hernández-Orts, Jesús S; Aznar, Francisco J; Blasco-Costa, Isabel; García, Néstor A; Víllora-Montero, María; Crespo, Enrique A; Raga, Juan A; Montero, Francisco E

    2013-08-29

    Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area.

  13. UJI EFEKTIVITAS NEMATODA ENTOMOPATOGEN (RHABDITIDA: Steinernema DAN Heterorhabditis SEBAGAI MUSUH ALAMI NON-ENDEMIK PENGGEREK BATANG PADI KUNING (Scirpophaga incertulas

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    Chaerani dan Bebet Nurbaeti .

    2012-02-01

    Full Text Available Efficacy Tests of Entomopathogenic Nematodes (Rhabditida:  Steinernema dan Heterorhabditis as Non-endemic Natural Enemies of Yellow Rice stem Borer (Scirpophaga incertulas. Yellow rice stem borer (Scirpophaga incertulas is a chronic insect pests of irrigated rice and difficult to control.  Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis are promising biological control agents for this pest as their infective juveniles (IJs are capable of seeking and infecting insect living in moist, cryptic habitat such as galleries created by stem borer larvae. Thirteen indigenous and exotic Steinernema and Heterorhabditis sprayed to rice seedlings in laboratory with nematodes at concentrations of 0.5 or 2.0×104 IJs ml-1 water caused larval mortality between 7–93%.  Further test in greenhouse on nematodes that had >50%  efficacy showed that an indigenous isolate, H. indicus INA H17, was the most effective among the tested nematodes in killing larvae or pupae (78%.  Reduction in plant damage caused by the insects could not be demonstrated as the trials was limited to potted plants. The survival ability of nematodes on rice plants was evaluated by using INA H4 as an example. A low percentage of INA H4 IJs (0.5% persisted in inner leaf sheath until 7 days post application, while IJs on leaf surface and outer leaf sheath survived only until 2 and 48 hours post application, respectively. Improvement of application strategies including repeated spray, addition of antidessicant and adjusment of spray volume and application at damage threshold or plant critical period are deemed necessary to enhance nematode efficacy and reduce plant damage in the field.

  14. Uji Efektivitas Nematoda Entomopatogen (Rhabditida: Steinernema Dan Heterorhabditis) Sebagai Musuh Alami Non-endemik Penggerek Batang Padi Kuning (Scirpophaga Incertulas)

    OpenAIRE

    Chaerani dan Bebet Nurbaeti

    2007-01-01

    Efficacy Tests of Entomopathogenic Nematodes (Rhabditida:  Steinernema dan Heterorhabditis) as Non-endemic Natural Enemies of Yellow Rice stem Borer (Scirpophaga incertulas). Yellow rice stem borer (Scirpophaga incertulas) is a chronic insect pests of irrigated rice and difficult to control.  Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis are promising biological control agents for this pest as their infective juveniles (IJs) are capable of seeking and infecting ins...

  15. Proteomic analysis of Oesophagostomum dentatum (Nematoda during larval transition, and the effects of hydrolase inhibitors on development.

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    Martina Ondrovics

    Full Text Available In this study, in vitro drug testing was combined with proteomic and bioinformatic analyses to identify and characterize proteins involved in larval development of Oesophagostomum dentatum, an economically important parasitic nematode. Four hydrolase inhibitors ο-phenanthroline, sodium fluoride, iodoacetamide and 1,2-epoxy-3-(pnitrophenoxy-propane (EPNP significantly inhibited (≥90% larval development. Comparison of the proteomic profiles of the development-inhibited larvae with those of uninhibited control larvae using two-dimensional gel electrophoresis, and subsequent MALDI-TOF mass spectrometric analysis identified a down-regulation of 12 proteins inferred to be involved in various larval developmental processes, including post-embryonic development and growth. Furthermore, three proteins (i.e. intermediate filament protein B, tropomyosin and peptidyl-prolyl cis-trans isomerase inferred to be involved in the moulting process were down-regulated in moulting- and development-inhibited O. dentatum larvae. This first proteomic map of O. dentatum larvae provides insights in the protein profile of larval development in this parasitic nematode, and significantly improves our understanding of the fundamental biology of its development. The results and the approach used might assist in developing new interventions against parasitic nematodes by blocking or disrupting their key biological pathways.

  16. Heterorhabditis beicherriana n.sp. (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Shunyi district of Beijing, China

    Czech Academy of Sciences Publication Activity Database

    Li, X.-Y.; Liu, Q.-Z.; Nermuť, Jiří; Půža, Vladimír; Mráček, Zdeněk

    -, č. 3569 (2012), s. 25-40 ISSN 1175-5326 R&D Projects: GA MŠk(CZ) ME10078 Grant - others:China Agriculture Research System(CN) CARS -29; China commonweal funds(CN) 201003043-02; China commonweal funds(CN) 201004037 Institutional support: RVO:60077344 Keywords : new species * description * morphology Subject RIV: EG - Zoology Impact factor: 0.974, year: 2012 http://www.mapress.com/zootaxa/2012/f/z03569p040f.pdf

  17. Paraspidodera uncinata (Nematoda, Lauroiinae as parasite of Cavia magna and Cavia aperea (Rodentia, Caviidae in southern Brazil

    Directory of Open Access Journals (Sweden)

    Ana Gabriela da Silva Rocha

    2015-05-01

    Full Text Available Out of the 5 species of Guinea pig registered for Brazil, 4 (Cavia fulgida, C. porcellus, C. aperea, and C. magna have already been reported as hosts of Paraspidodera uncinata. The rodent species C. magna and C. aperea are small-sized mammals, with terrestrial habits, which occur from southern Brazil to eastern Uruguay. Guinea pig specimens donated for research were necropsied for the analysis of endoparasites, and the nematodes found were identified as P. uncinata. The microhabitats of this species were the small and large intestines in C. magna and the large intestine in C. aperea. The prevalence of P. uncinata in the hosts was 60% in C. magna (n = 5 and 14% in C. aperea (n = 7. The record of P. uncinata in different Guinea pig species allows inferring that the foraging mode is similar in the different locations where they occur in southern Brazil, because, perhaps, the infection of hosts has occurred through the ingestion of vegetables contaminated with P. uncinata eggs. This study contributes to knowledge on the helminth fauna of C. magna and C. aperea in southern Brazil.

  18. Interactions between the entomopathogenic nematode Heterorhabditis sonorensis (Nematoda: Heterorhabditidae) and the saprobic fungus Fusarium oxysporum (Ascomycota: Hypocreales).

    Science.gov (United States)

    Navarro, P D; McMullen, J G; Stock, S P

    2014-01-01

    In this study, we assessed the effect of the saprobic fungus, Fusarium oxysporum (Ascomycota: Hypocreales) on the fitness of the entomopathogenic nematode Heterorhabditis sonorensis (Caborca strain). Sand column assays were considered to evaluate the effect of fungal mycelia on infective juvenile (IJ) movement and host access. Additionally, we investigated the effect of fungal spores on the nematodes' ability to search for a host, its virulence, penetration efficiency and reproduction. Three application timings were considered to assess interactions between the fungus and the nematodes. In vitro assays were also considered to determine the effect of fungal extracts on the nematode's symbiotic bacteria. Our observations indicate that presence and age of fungal mycelia significantly affect IJ movement in the sand columns and their ability to establish in the host. These results were also reflected in a reduced insect mortality. In particular, treatments with the 15 days old mycelia showed a significant reduction in insect mortality and penetration efficiency. Presence of fungal spores also impacted nematode virulence and reproduction. In particular, two of the application timings tested (simultaneous [EPN and fungal spores applied at the same time] and alternate I [EPN applied first, fungus applied 24h later]) resulted in antagonistic interactions. Moreover, IJ progeny was reduced to half in the simultaneous application. In vitro assays revealed that fungal extracts at the highest concentration tested (10mg/ml) inhibited the growth of the symbiotic bacteria. Overall, these results suggest that saprobic fungi may play an important role in regulating. EPN populations in the soil, and that they may be one of the factors that impact nematode survival in the soil and their access to insect hosts. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. Occurrence of Terranova larval types (Nematoda: Anisakidae in Australian marine fish with comments on their specific identities

    Directory of Open Access Journals (Sweden)

    Shokoofeh Shamsi

    2016-03-01

    Full Text Available Pseudoterranovosis is a well-known human disease caused by anisakid larvae belonging to the genus Pseudoterranova. Human infection occurs after consuming infected fish. Hence the presence of Pseudoterranova larvae in the flesh of the fish can cause serious losses and problems for the seafood, fishing and fisheries industries. The accurate identification of Pseudoterranova larvae in fish is important, but challenging because the larval stages of a number of different genera, including Pseudoterranova, Terranova and Pulchrascaris, look similar and cannot be differentiated from each other using morphological criteria, hence they are all referred to as Terranova larval type. Given that Terranova larval types in seafood are not necessarily Pseudoterranova and may not be dangerous, the aim of the present study was to investigate the occurrence of Terranova larval types in Australian marine fish and to determine their specific identity. A total of 137 fish belonging to 45 species were examined. Terranova larval types were found in 13 species, some of which were popular edible fish in Australia. The sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2 respectively of the Terranova larvae in the present study showed a high degree of similarity suggesting that they all belong to the same species. Due to the lack of a comparable sequence data of a well identified adult in the GenBank database the specific identity of Terranova larval type in the present study remains unknown. The sequence of the ITS regions of the Terranova larval type in the present study and those of Pseudoterranova spp. available in GenBank are significantly different, suggesting that larvae found in the present study do not belong to the genus Pseudoterranova, which is zoonotic. This study does not rule out the presence of Pseudoterranova larvae in Australian fish as Pseudoterranova decipiens E has been reported in adult form from seals in Antarctica and it is known that they have seasonal presence in Australian southern coasts. The genetic distinction of Terranova larval type in the present study from Pseudoterranova spp. along with the presence of more species of elasmobranchs in Australian waters (definitive hosts of Terranova spp. and Pulchrascaris spp. than seals (definitive hosts of Pseudoterranova spp. suggest that Terranova larval type in the present study belong to either genus Terranova or Pulchrascaris, which are not known to cause disease in humans. The present study provides essential information that could be helpful to identify Australian Terranova larval types in future studies. Examination and characterisation of further specimens, especially adults of Terranova and Pulchrascaris, is necessary to fully elucidate the identity of these larvae.

  20. Barracudia australiensis n. g., n. sp (Nematoda: Philometridae) from the obtuse barracuda Sphyraena obtusata Cuvier (Perciformes: Sphyraenidae) off eastern Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Shamsi, S.

    2017-01-01

    Roč. 94, č. 6 (2017), s. 627-634 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : yamaguti * fishes * coast Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine OBOR OECD: Veterinary science Impact factor: 1.181, year: 2016

  1. The genus Discolaimus in Southern Africa. I. Introduction and redescription of D. krugeri Furstenberg & Heyns, 1966 (Nematoda: Dorylaimida

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    J. Heyns

    2000-07-01

    Full Text Available A survey of the literature leads to the conclusion that males are rare or absent in about half of the known species of Discolaimus and probably seldom functional, except in two species where spermathecae are present in the female reproductive system, viz. D. krugeri and D. levinae. The structure of the female gonads and the location of the oesophageal gland nuclei are discussed. A redescription of D. krugeri is given, based on material from several localities in South Africa as well as a single male from Namibia.

  2. Capillostrongyloides arapaimae sp. n. (Nematoda: Capillariidae, a new intestinal parasite of the arapaima Arapaima gigas from the Brazilian Amazon

    Directory of Open Access Journals (Sweden)

    Cláudia Portes Santos

    2008-06-01

    Full Text Available A new nematode species, Capillostrongyloides arapaimae sp. n., is described from the intestine and pyloric caeca of the arapaima, Arapaima gigas (Schinz, from the Mexiana Island, Amazon river delta, Brazil. It is characterized mainly by the length of the spicule (779-1,800 µm, the large size of the body (males and gravid females 9.39-21.25 and 13.54-27.70 mm long, respectively and by the markedly broad caudal lateral lobes in the male. It is the third species of genus Capillostrongyloides reported to parasitize Neotropical freshwater fishes.

  3. Capillostrongyloides arapaimae sp. n. (Nematoda: Capillariidae), a new intestinal parasite of the arapaima Arapaima gigas from the Brazilian Amazon.

    Science.gov (United States)

    Santos, Cláudia Portes; Moravec, Franti Ek; Venturieri, Rossana

    2008-06-01

    A new nematode species, Capillostrongyloides arapaimae sp. n., is described from the intestine and pyloric caeca of the arapaima, Arapaima gigas (Schinz), from the Mexiana Island, Amazon river delta, Brazil. It is characterized mainly by the length of the spicule (779-1,800 microm), the large size of the body (males and gravid females 9.39-21.25 and 13.54-27.70 mm long, respectively) and by the markedly broad caudal lateral lobes in the male. It is the third species of genus Capillostrongyloides reported to parasitize Neotropical freshwater fishes.

  4. Capillostrongyloides arapaimae sp. n. (Nematoda: Capillariidae), a new intestinal parasite of the arapaima Arapaima gigas from the Brazilian Amazon

    OpenAIRE

    Santos, Cláudia Portes; Moravec, František; Venturieri, Rossana

    2008-01-01

    A new nematode species, Capillostrongyloides arapaimae sp. n., is described from the intestine and pyloric caeca of the arapaima, Arapaima gigas (Schinz), from the Mexiana Island, Amazon river delta, Brazil. It is characterized mainly by the length of the spicule (779-1,800 µm), the large size of the body (males and gravid females 9.39-21.25 and 13.54-27.70 mm long, respectively) and by the markedly broad caudal lateral lobes in the male. It is the third species of genus Capillostrongyloides ...

  5. Capillostrongyloides arapaimae sp. n. (Nematoda: Capillariidae), a new intestinal parasite of the arapaima Arapaima gigas from the Brazilian Amazon

    Czech Academy of Sciences Publication Activity Database

    Santos, C. P.; Moravec, František; Venturieri, R.

    2008-01-01

    Roč. 103, č. 4 (2008), s. 392-395 ISSN 1678-8060 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Capillostrongyloides * Arapaima * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine

  6. New data on the morphology of some Far-Eastern species of Rhabdochona (Nematoda: Rhabdochonidae), as revealed by SEM observations

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Ermolenko, A. V.; Besprozvannykh, V. V.; Scholz, Tomáš

    2012-01-01

    Roč. 59, č. 3 (2012), s. 195-208 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematodes * Rhabdochona * freshwater fish * Chanodichthys * Culter * Hemibarbus * Russian Far East Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/detail.php?id=22067

  7. Experimental population dynamics of Rhabdias bufonis (Nematoda) in toads (Bufo bufo): density-dependence in the primary infection

    OpenAIRE

    Goater, C. P.

    2017-01-01

    Density-dependence in worm establishment, numbers, biomass and larval production were examined in primary infections of 0, 10, 40, 80 and 160 larvae of the lung nematode, Rhabdias bufonis in the common toad, Bufo bufo. The infection procedure established 4 non-overlapping levels of infection which persisted until 6 weeks post-infection (p.i.), after which there was an overall decline up to 12 weeks p.i. Worm numbers had no direct effect on adult worm survival but temporal changes in worm weig...

  8. Neoparaseuratum travasssosi, n. g., n. sp. (Nematoda: Quimperiidae, a new parasite from thorny catfish Pterododas granulosus in Brazil

    Directory of Open Access Journals (Sweden)

    F. Moravec

    1992-01-01

    Full Text Available A new nematode genus and species. Neoparaseuratum travassosi n. g., n. sp., is described from the intestine of the freshwater thorny catfish, Pterodoras granulosus (Valenciennes, from the Paraná River, Brazil. This seuratoid nematode species represents a new genus of the family Quimperiidae, being characterized mainly by the presence of numerous narrow longitudinal bands of inflated cuticle extending along the cephalic region of the body, small deirids, postoesophageal position of the excretory pore, relatively short (0.159-0.303 mm, equal spicules and a gubernaculum, the absence of caudal alae and preanal sucker in the male, and by some other features.

  9. Anthelminthic activity of methanol extracts of Diospyros anisandra and Petiveria alliacea on cyathostomin (Nematoda: Cyathostominae) larval development and egg hatching.

    Science.gov (United States)

    Flota-Burgos, G J; Rosado-Aguilar, J A; Rodríguez-Vivas, R I; Arjona-Cambranes, K A

    2017-12-15

    Methanol extracts of plant structures are promising alternatives to traditional pharmaceutical anthelminthic treatments. An in vitro evaluation was done of how methanol extracts of Diospyros anisandra bark and leaves, and Petiveria alliacea stems and leaves, collected during the rainy and dry seasons, effected cyathostomin larval development and egg hatching. Seven concentrations (600, 300, 150, 75, 37.5, 18.7 and 9.3μg/ml) were tested using the egg hatch assay. An ANOVA was applied to identify differences between the concentrations and the controls. Fifty percent lethal concentration (LC 50 ) and the 95% confidence interval were calculated with a probit analysis. At and above 37.5μg/ml, the D. anisandra bark extracts from both seasons exhibited ≥95% egg hatch inhibition (EHI), while the D. anisandra leaf extracts had >90% EHI at and above 75μg/ml. For P. alliacea, the extracts from leaves and stems from either season exhibited >97% EHI at and above 300μg/ml, although similar efficacy was also observed at lower concentrations with the rainy season stems (75μg/ml) and leaves (150μg/ml). Values for LC 50 were lowest for the rainy season D. anisandra bark (10.2μg/ml) and leaf extracts (18.4μg/ml), followed by the rainy season P. alliacea stems extract (28.2μg/ml). In the D. anisandra extracts, EHI was largely due to its ovicidal activity (≥96% beginning at 37.5μg/ml), whereas in the P. alliacea extracts it was due to L 1 larval hatch failure (≥90% beginning at 75μg/ml). Overall, the rainy season D. anisandra bark extracts had a strong in vitro anthelminthic effect against cyathostomins by inhibiting larval development, and the rainy season P. alliacea stem extracts had a strong effect by preventing egg hatching. Both are possible control alternatives for these nematodes. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Free-living nematode species (Nematoda) dwelling in hydrothermal sites of the North Mid-Atlantic Ridge

    Science.gov (United States)

    Tchesunov, Alexei V.

    2015-12-01

    Morphological descriptions of seven free-living nematode species from hydrothermal sites of the Mid-Atlantic Ridge are presented. Four of them are new for science: Paracanthonchus olgae sp. n. (Chromadorida, Cyatholaimidae), Prochromadora helenae sp. n. (Chromadorida, Chromadoridae), Prochaetosoma ventriverruca sp. n. (Desmodorida, Draconematidae) and Leptolaimus hydrothermalis sp. n. (Plectida, Leptolaimidae). Two species have been previously recorded in hydrothermal habitats, and one species is recorded for the first time in such an environment. Oncholaimus scanicus (Enoplida, Oncholaimidae) was formerly known from only the type locality in non-hydrothermal shallow milieu of the Norway Sea. O. scanicus is a very abundant species in Menez Gwen, Lucky Strike and Lost City hydrothermal sites, and population of the last locality differs from other two in some morphometric characteristics. Desmodora marci (Desmodorida, Desmodoridae) was previously known from other remote deep-sea hydrothermal localities in south-western and north-eastern Pacific. Halomonhystera vandoverae (Monhysterida, Monhysteridae) was described and repeatedly found in mass in Snake Pit hydrothermal site. The whole hydrothermal nematode assemblages are featured by low diversity in comparison with either shelf or deep-sea non-hydrothermal communities. The nematode species list of the Atlantic hydrothermal vents consists of representatives of common shallow-water genera; the new species are also related to some shelf species. On the average, the hydrothermal species differ from those of slope and abyssal plains of comparable depths by larger sizes, diversity of buccal structures, presence of food content in the gut and ripe eggs in uteri.

  11. Characterization of four species of Trichuris (Nematoda: Enoplida) by their second internal transcribed spacer ribosomal DNA sequence.

    Science.gov (United States)

    Oliveros, R; Cutillas, C; De Rojas, M; Arias, P

    2000-12-01

    Adult worms of Trichuris ovis and T. globulosa were collected from Ovis aries (sheep) and Capra hircus (goats). T. suis was isolated from Sus scrofa domestica (swine) and T. leporis was isolated from Lepus europaeus (rabbits) in Spain. Genomic DNA was isolated and a ribosomal internal transcribed spacer (ITS2) was amplified and sequenced using polymerase-chain-reaction (PCR) techniques. The ITS2 of T. ovis and T. globulosa was 407 nucleotides in length and had a GC content of about 62%. Furthermore, the ITS2 of T. suis and T. leporis was 534 and 418 nucleotides in length and had a GC content of about 64.8% and 62.4%, respectively. There was evidence of slight variation in the sequence within individuals of all species analyzed, indicating intraindividual variation in the sequence of different copies of the ribosomal DNA. Furthermore, low-level intraspecific variation was detected. Sequence analyses of ITS2 products of T. ovis and T. globulosa demonstrated no sequence difference between them. Nevertheless, differences were detected between the ITS2 sequences of T. suis, T. leporis, and T. ovis, indicating that Trichuris species can reliably be differentiated by their ITS2 sequences and PCR-linked restriction-fragment-length polymorphism (RFLP).

  12. Lobocapillaria austropacifica n. g., n. sp (Nematoda: Capillariidae) from the obtuse barracuda Sphyraena obtusata Cuvier (Sphyraenidae, Perciformes) off eastern Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Beveridge, I.

    2017-01-01

    Roč. 94, č. 5 (2017), s. 547-556 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : intestinal parasite * new-caledonia Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.181, year: 2016

  13. Description of Caenorhabditis sinica sp. n. (Nematoda: Rhabditidae, a nematode species used in comparative biology for C. elegans.

    Directory of Open Access Journals (Sweden)

    Ren-E Huang

    Full Text Available We re-isolated in China a relative of the nematode model Caenorhabditis elegans that was previously referred to informally as C. sp. 5. In spite of its importance for comparative biology, C. sp. 5 has remained morphologically uncharacterized. Therefore, we now provide detailed description of morphology and anatomy, assigning the name of Caenorhabditis sinica sp. n. to this nematode that is found frequently in China. C. sinica sp. n. belongs to the Elegans group in the genus Caenorhabditis, being phylogenetically close to C. briggsae although differing in reproductive mode. The gonochoristic C. sinica sp. n. displays two significantly larger distal parts of uteri filled with sperms in the female/hermaphroditic gonad than does the androdioecious C. briggsae. The new species can be differentiated morphologically from all known Caenorhabditis species within the Elegans group by presenting a uniquely shaped, three-pointed hook structure on the male precloacal lip. The lateral field of C. sinica sp. n. is marked by three ridges that are flanked by two additional incisures, sometimes appearing as five ridges in total. This study ends the prolonged period of the 'undescribed' anonymity for C. sinica sp. n. since its discovery and use in comparative biological research. Significant and crossing-direction dependent hybrid incompatibilities in F1 and F2 crossing progeny make C. sinica sp. n. an excellent model for studies of population and speciation genetics. The abundance of nematode species lacking detailed taxonomic characterization deserves renewed attention to address the species description gap for this important yet morphologically 'difficult' group of animals.

  14. Methods for estimating the density of Elaphostrongylus rangiferi Mitskevich (Nematoda, Metastrongyloidea larvae in faeces from reindeer, Rangifer tarandus L.

    Directory of Open Access Journals (Sweden)

    Odd Halvorsen

    1983-05-01

    Full Text Available A method for estimating the density of Elaphostrongylus rangiferi larvae in reindeer faeces that have been deep frozen is described. The method involves the use of an inverted microscope with plankton counting chambers. Statistical data on the efficiency and sensitivity of the method are given. With fresh faeces, the results obtained with the method were not significantly different from those obtained with the Baermann technique. With faeces that had been stored in deep freeze, the method detected on average 30 per cent more larvae than the Baermann technique.Metoder for å estimere tettheten av hjernemarklarver i avføring fra reinsdyr.Abstract in Norwegian / Sammendrag: En metode for å estimere tettheten av hjernemarklarver i avføring som har vært dypfryst blir beskrevet. Anvendelse av et invertert mikroskop med plankton tellekammer inngår i metoden. Det blir gitt statistiske data for metodens effektivitet og følsomhet. Ved undersøkelse av fersk avføring skilte ikke de resultatene metoden ga seg fra de som ble oppnådd med Baermanns metode. Ved undersøkelse av avføring som hadde vært lagret dypfrosset ga metoden i gjennomsnitt 30 prosent flere larver enn Baermanns metode.

  15. New data on three gonad-infecting species of Philometra (Nematoda, Philometridae) from estuarine fishes in South Carolina, USA

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; de Buron, I.

    2009-01-01

    Roč. 54, č. 3 (2009), s. 244-252 ISSN 1230-2821 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Estuarine fishes * USA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.070, year: 2009

  16. Female morphology of Philometra parasiluri (Nematoda, Philometridae), an ocular parasite of the Amur catfish Silurus asotus in Japan

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Scholz, Tomáš; Kuchta, Roman; Grygier, M. J.

    2008-01-01

    Roč. 53, č. 2 (2008), s. 153-157 ISSN 1230-2821 R&D Projects: GA MŠk LC522; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Silurus * Japan Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.748, year: 2008

  17. A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient.

    Science.gov (United States)

    Mladineo, Ivona; Popović, Marijana; Drmić-Hofman, Irena; Poljak, Vedran

    2016-02-01

    This is the first report of anisakiasis in a Croatian patient, evidenced from an archival paraffin-embedded and hematoxylin-eosin stained tissue section. Anisakiasis has been only suspected in the country based on previously detected anti-Anisakis IgE seroprevalence in the healthy coastal population, as well as an acute case where pathohistological and serological findings suggested the diseases, but the migrating larva has not been retrieved. Seventy years-old female, operated in 1998 for pulmonary carcinoma, was admitted to the General hospital Šibenik, Croatia in 2003, because of gastric pain and nausea that lasted for couple of days. She was showing good general condition, full mobility and lucidity, subfebrile status. Abdominal palpation inferred acute pain in paraumbilical and ileocecal region. Exploratory right pararectal laparotomy revealed a hardened, 5 cm-long structure, located intraluminally in the sigmoid colon, not perforating colon serosa. The process has been dissected and sent for patohistological diagnosis. Results showed a 2 mm-long whitish nematode spiralised in muscular layer of colon mucosa surrounded by granulomatous inflammation. After genomic DNA isolation of the nematode from the histological section, and amplification at the mitochondrial cytochrome oxidase 2 locus, etiological agent has been identified as Anisakis pegreffii. Used methodology suggests that screening of archival suspicious sections is feasible in order to study epidemiology of this zoonotic disease poorly recognised in Croatia.

  18. First report of Seuratascaris numidica Seurat, 1917 (Nematoda: Ascarididae) and other helminths from amphibians of West Bengal, India.

    Science.gov (United States)

    Sou, Sujan K; Bursey, Charles R

    2017-03-01

    Studies on nematode parasites from anuran hosts from Eastern India, particularly West Bengal, are rare. To our knowledge, there is a report of larvae of Monhysterides sp. (a fish parasite) from cysts within the body cavity and musculature of Duttaphrynus melanostictus, Hoplobatrachus tigerinus and Euphlyctis hexadactylus as well as descriptions of Aplectana duttaphryni from D. melanostictus , Aplectana dubrajpuri from H. tigerinus , Cosmocerca microhylae from Microhyla rubra and Rhabdias bulbicauda from D. melanostictus . Here, we report mature individuals of typical anuran parasites, Diplodiscus amphichrus and Seuratascaris numidica , from H. tigerinus and larvae of Acuariidae (a bird parasite) from Fejervarya limnocharis collected in Kulti, West Bengal.

  19. Oxyuroids of palearctic Testudinidae--new definition for Alaeuris Seurat, 1918 (Nematoda: Pharyngodonidae) and redescription of Alaeuris numidica (Seurat, 1918).

    Science.gov (United States)

    Bouamer, S; Morand, S; Bourgat, R

    2001-02-01

    The generic diagnosis of Alaeuris is emended based on the study and redescription of Alaeuris numidica from the cecum of Testudo graeca collected in Settat, Morocco and of Testudo hermanni collected in Catalonia, Spain. Scanning electron microscopy studies revealed that the papillae previously described as adanal are simple lobes because of the lack of terminal nerves, and that both preanal and postanal papillae are pedunculate. These new findings allowed us to emend the diagnosis of the genus and the species. A list of species of Alaeuris with biogeographic regions and hosts is provided.

  20. New morphological data on the first-stage larvae of two Procamallanus species (Nematoda: Camallanidae) based on SEM studies

    Czech Academy of Sciences Publication Activity Database

    Mašová, Š.; Baruš, Vlastimil; Moravec, František

    2011-01-01

    Roč. 58, č. 4 (2011), s. 318-321 ISSN 0015-5683 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60930519; CEZ:AV0Z60220518 Keywords : Procamallanus * freshwater fish * Kenya Subject RIV: EG - Zoology Impact factor: 1.812, year: 2011 http://www.paru.cas.cz/folia/pdfs/showpdf.php?pdf=22007

  1. Note on morphology of two nematode species Ascaridia hermaphrodita and Ascaridia platyceri (Nematoda): scanning electron microscope study

    Czech Academy of Sciences Publication Activity Database

    Hodová, I.; Baruš, Vlastimil; Tukač, V.

    2008-01-01

    Roč. 45, č. 3 (2008), s. 109-113 ISSN 0440-6605 Institutional research plan: CEZ:AV0Z60220518 Keywords : Ascaridia hermaphrodita * Ascaridia platyceri * Psittaciformes * Czech Republic * morphology Subject RIV: EG - Zoology Impact factor: 0.443, year: 2008

  2. Redescription of Cystidicoloides fischeri based on specimens from piranhas in Brazil, and erection of a new genus (Nematoda: Cystidicolidae)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Santos, M. D.; Brasil-Sato, M. C.

    2008-01-01

    Roč. 94, č. 4 (2008), s. 889-897 ISSN 0022-3395 R&D Projects: GA MŠk LC522; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cystidicoloides * Serrasalmidae * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.165, year: 2008

  3. A new species of Hysterothylacium (Nematoda: Anisakidae) from the giant mottled eel Anguilla marmorata in South Africa

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Taraschewski, H.; Appelhoff, D.; Weyl, O.

    2012-01-01

    Roč. 49, č. 3 (2012), s. 174-180 ISSN 0440-6605 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Hysterothylacium * Anguilla * South Africa Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.783, year: 2012 http://link.springer.com/article/10.2478%2Fs11687-012-0035-9

  4. Dos nuevas especies de Stilestrongylus (Nematoda: Heligmonellidae parásitos de peromyscus (Rodentia: Cricetidae de México

    Directory of Open Access Journals (Sweden)

    Jorge Falcón Ordaz

    1999-12-01

    Full Text Available Se describen dos especies nuevas del género Stilestrongylus Freitas, Lent & Almeida, 1937, parásitas de Peromyscus spp. (Rodentia: Cricetidae provenientes del Estado de Hidalgo, Mexico. Stilestrongylus peromysci n. sp. infecta a Peromyscus difficilis y se caracteriza por la presencia de 30 espínas en el synlophe para ambos sexos, así como por el nacimiento simétrico de los rayos 8 a partir de la raíz del rayo nueve. S. hidalguensis n. sp. parásita a Peromyscus sp., diferenciándose del resto de las especies congenéricas porque el macho presenta 24 espinas en el synlophe a nivel de la parte media del cuerpo y porque el arreglo de los rayos bursales es diferente en ambos lóbulos (2-2-1 derecho y 2-3 izquierdo. Se presenta una clave para la identificación de 18 de las 19 especies del género.Stilestrongylus peromysci n. sp. collected from Peromyscus difficilis (Hidalgo state, México, differs from other species in the genus in number of the spines (30 in the synlophe (both sexes and because the eigth ray arises from the root of the ninth ray; S. hidalguensis n. sp. parasited Peromyscus sp. and differs from all other congeneric species in the presence of 24 spines in the male synlophe and in the arrangement of the bursal rays (2-2-1 in the right lobe and 2-3 in the left lobe. A key to the species of Stilestrongylus is provided.

  5. Occurrence of parasites of the genus Eustrongylides spp. (Nematoda: Dioctophymatidae in fish caught in Trasimeno lake, Italy

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    Raffaella Branciari

    2016-11-01

    Full Text Available Eustrongylides spp. is considered a freshwater fish zoonotic nematode. In the present study, the prevalence of Eustrongylides spp. in six edible fish (European perch - Perca fluviatilis, goldfish - Carassius auratus, largemouth black bass - Micropterus salmoides, tench- Tinca tinca, carp - Cyprinus carpio and sand smelt - Atherina boyeri of Trasimeno lake was surveyed. The investigations were conducted from October 2014 to September 2015 and 384 specimens per species for each season were caught in Trasimeno lake and examined for the presence of larvae in the abdominal cavity and muscle. The presence of nematodes in the abdominal cavity and musculature was revealed in three fish species. The prevalence of Eustrongylides spp. infection was 6.84, 1.89 and 0.13% in perch, largemouth black bass and sand smelt, respectively. The number of parasites per fish was only one in largemouth black bass and sand smelt and ranged from one up to three in perch. This study states that the European perch, largemouth black bass and sand smelt of Trasimeno lake are infected with zoonotic parasites; therefore, food business operators have to take appropriate measures to guarantee the health of consumers.

  6. The first record of Dracunculus mulbus (Nematoda: Dracunculidae) in the Papuan olive python Apodora papuana (Ophidia: Boidae)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Gibson, D. I.

    2007-01-01

    Roč. 44, č. 3 (2007), s. 118-119 ISSN 0440-6605 R&D Projects: GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Python * Dracunculus * Papua New Guinea Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.373, year: 2007

  7. Pseudocapillaria (Ichthyocapillaria) ophisterni sp. n. (Nematoda : Capillariidae) from the swamp-eel Ophisternon aenigmaticum (Pisces) in Mexico.

    Science.gov (United States)

    Moravec, F; Salgado-Maldonado, G; Jiménez-García, I

    2000-04-01

    A new nematode species, Pseudocapillaria ophisterni sp. n., is described from the intestine and rarely from the stomach of the swamp-eel, Ophisternon aenigmaticum Rosen et Greenwood, from Catemaco Lake, Veracruz, Mexico. In having both caudal lobes in the male interconnected by a distinct dorsal membrane, it belongs to the subgenus Ichthyocapillaria. It differs from the three species in this subgenus mainly in possessing either a distinctly longer spicule or a smaller length of oesophagus relative to body length. It also differs in host type and geographical distribution. P. ophisterni is the first capillariid species reported from synbranchiform fishes.

  8. Influence of different forages on gastrointestinal namatode infections in grazing lambs

    OpenAIRE

    Thamsborg, S.M.; Mejer, H.; Bandier, M.; Larsen, M.

    2003-01-01

    Nematode infections of sheep may be influenced by secondary compounds in the diet, e.g. condensed tannins. A study was performed with 7 groups of lambs experimentally infected with Teladorsagia circumcincta and Trichostronglylus vitrinus. All groups were grazed on clean clover-grass pasture and then moved to paddocks with bioactive forages with either sainfoin (Onobrychis viciifolia) (groups Pre-S and Post-S), chicory (Cichorium intybus, cv. Grasslands Puna) (Pre-C and Post-C) or clover-grass...

  9. A new gonad-infecting species of Philometra (Nematoda: Philometridae) from the red cusk-eel Genypterus chilensis (Osteichthyes: Ophidiidae) off Chile

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Chávez, R. A.; Oliva, M. E.

    2011-01-01

    Roč. 108, č. 1 (2011), s. 227-232 ISSN 0932-0113 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Genypterus * Chile Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.149, year: 2011

  10. Raphidascaris (Raphidascaris) macrouri n. sp (Nematoda: Anisakidae) from two deep-sea macrourid fishes in the Western Mediterranean: Morphological and molecular characterisations

    Czech Academy of Sciences Publication Activity Database

    Pérez-i-García, D.; Constenla, M.; Carrasson, M.; Montero, F. E.; Soler-Membrives, A.; González-Solís, David

    2015-01-01

    Roč. 64, č. 5 (2015), s. 345-352 ISSN 1383-5769 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Raphidascaris (Raphidascaris) macrouri * deep-water * Macrouridae * Nezumia aequalis * Trachyrincus scabrus * Spain Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.860, year: 2015

  11. Heliconema longissimum (Ortlepp, 1923) (Nematoda: Physalopteridae) from Pisodonophis boro (Teleostei: Ophichthidae) in Thailand, with remarks on the taxonomy of the Proleptinae Schulz, 1927

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Taraschewski, H.; Thairungroj Anantaphruti, M.; Maipanich, W.; Laoprasert, T.

    2007-01-01

    Roč. 66, č. 1 (2007), s. 73-80 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Heliconema * Pisodonophis * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.125, year: 2007

  12. Durettenema guangdongense gen. et sp. nov. (Nematoda: Molineoidea) from Hipposideros larvatus (Horsfield) (Chiroptera: Rhinolophidae) with discussion of the taxonomic status of Macielia rhinolophi Yin, 1980.

    Science.gov (United States)

    Ju, Hui-Dong; Li, Liang; Zhang, Lu-Ping

    2017-09-26

    Durettenema guangdongense gen. et sp. nov. is described from Hipposideros larvatus (Horsfield) (Chiroptera: Rhinolophidae) in Guangdong Province, China. The new genus differs from the other genera of subfamily Molineinae in the structure of the synlophe, the absence of lateral alae, the arrangement of the bursa rays, the shape of the spicules, the female tail and the presence of gubernaculum. Meanwhile, considering the morphological characters of Macielia rhinolophi Yin, 1980, including the pattern of the bursa ray, the shape of the spicules, and the female tail, this species should be transferred to the genus Durettenema, as D. rhinolophi (Yin, 1980) comb. nov., which can be distinguished from D. guangdongense in the shape of the ovejector. In addition, the ITS-1 sequences of D. guangdongense were also analysed, these sequence added new data for the molecular diagnosis of trichostrongylid nematodes.

  13. MORTALITY OF YOUTH Arapaima gigas (PISCES: ARAPAIMIDAE FROM A FISH FARMING IN THE NORTH OF BRAZIL, CAUSED BY Hysterothylacium sp. AND Goezia spinulosa (NEMATODA: ANISAKIDAE

    Directory of Open Access Journals (Sweden)

    Patrizia Batista de Azevedo

    2017-03-01

    Full Text Available In youth A. gigas the risk of parasitic infections is very high, due to their feeding habit to prey on small invertebrates, which act as intermediate hosts of different endoparasite species, which can cause serious problems and high mortalities in fish farms. Thus, the aim of the present study was to identify the species that parasitize youth A. gigas raised in captivity in the Manacapuru municipality, Amazonas State and to evaluate their influence on the mortality of these fish. There were examined 66 youth A. gigas and non-parasite was recorded in the host gills. The intestine and stomach of the fish were parasitized by Hysterothylacium sp. larvae (L3 and Goezia spinulosa larvae (L4. The parasitic indexes were high for the two species, being recorded the highest rates of infection by Hysterothylacium sp. There was observed a weak positive correlation between the host standard length and the abundance of Hysterothylacium sp. The lesions observed in the stomach and intestine of the fish, together with the high parasite index values recorded for Hysterothylacium sp. and Goezia spinulosa, leads to the suspicion that the death of the fish was due to complications and damages caused by the presence of these parasites. Keywords: Amazonia; arapaima; death; nematodes.

  14. Toxocara (Nematoda: Ascaridida and Other Soil-Transmitted Helminth Eggs Contaminating Soils in Selected Urban and Rural Areas in the Philippines

    Directory of Open Access Journals (Sweden)

    Vachel Gay V. Paller

    2014-01-01

    Full Text Available The extent of contamination of soils with soil transmitted helminthes (STH eggs, particularly Toxocara, was determined in selected urban and rural towns of Laguna, Philippines. Soil samples were collected from public schools, house yards, and empty lots. Results revealed that, of the 1480 soil samples collected, 460 (31% were positive for STH eggs. Toxocara sp. was the most prevalent (77%, followed by Ascaris sp. (11%, hookworms/strongyles/free-living nematodes (7%, and Trichuris sp. (5%. Some soil physicochemical parameters were also determined and associated with Toxocara eggs prevalence and density in soil. Results revealed that Toxocara sp. eggs were most prevalent in less acidic, relatively high temperature and high moisture soil conditions. They were also prevalent in sandy, silty, and loamy soil textures but less prevalent in clayey. No significant differences were found between depth 1 (0–5 cm and depth 2 (6–10 cm. This study revealed that Toxocara sp. eggs are ubiquitous and the extent of contamination in soils from the selected towns of Laguna is relatively high. Hence, the data generated in this study can be used in promoting public awareness, particularly for pet owners and local health officials, for effective prevention and control of this parasitosis.

  15. Spinitectus aguapeiensis n. sp (Nematoda: Cystidicolidae) from Pimelodella avanhandavae Eigenmann (Siluriformes: Heptapteridae) in the River Aguapei, Upper Parana River Basin, Brazil

    Czech Academy of Sciences Publication Activity Database

    Acosta, A.A.; González-Solís, David; Da Silva, R.J.

    2017-01-01

    Roč. 94, č. 6 (2017), s. 649-656 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:60077344 Keywords : fresh-water fish * southern Mexico * intestine * osorioi * Chiapas Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine OBOR OECD: Veterinary science Impact factor: 1.181, year: 2016

  16. Redescription of Ichthyouris bursata Moravec & Prouza, 1995 (Nematoda: Pharyngodonidae), a parasite of wild and aquarium-reared discus Symphysodon spp. (Osteichthyes)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Laoprasert, T.

    2008-01-01

    Roč. 71, č. 1 (2008), s. 137-143 ISSN 0165-5752 R&D Projects: GA MŠk LC522; GA ČR(CZ) GA524/06/0170 Institutional research plan: CEZ:AV0Z60220518 Keywords : Ichthyouris * Symphysodon * Thailand Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.927, year: 2008

  17. An observational study on changes in biometry and generation time of Odontophora villoti (Nematoda, Axonolaimidae) related to petroleum pollution in Bizerte bay, Tunisia.

    Science.gov (United States)

    Boufahja, Fehmi; Hedfi, Amor; Essid, Naceur; Aïssa, Patricia; Mahmoudi, Ezzeddine; Beyrem, Hamouda

    2012-03-01

    We conducted a yearly polluted-reference sampling to assess the effects of petroleum pollution on life cycle characteristics of the meiobenthic nematode Odontophora villoti. Samples were taken every 15 days between 26 November 2004 and 25 November 2005 from two beaches of Bizerte bay (Tunisia), Rimel and Tunisian Refining Industries Company (TRIC). The latter site is located in front of the "Tunisian Refining Industries Company" runoff. When compared to the reference site, the mean body dimensions of O. villoti from the impacted site were significantly lower. The small size of affected nematodes was represented both by the length and width as a function of the life stage. It was also established that changes in lengths of body parts during molts were different between the two study sites. The low availability of oxygen from April to August seems to prevent the formation of embryos of O. villoti. Thus, two annual reproductive cycles with different durations were observed in Rimel and TRIC. Under stress, juvenile phase and egg production were generally shorter. Globally, the impact of petroleum pollution on O. villoti was expressed by a short egg-to-egg development time. Our study assessed the usefulness of life cycle characteristics (biometry and life stage durations) of O. villoti in biomonitoring, and the results are generally consistent suggesting that this species may be considered as an efficient bioindicator.

  18. Two new species of Philometra Costa, 1845 (Nematoda: Philometridae) from Mycteroperca spp. (Serranidae) in the North-West Atlantic and northern Gulf of Mexico, USA

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Bakenhaster, M.; Adams, D.H.

    2016-01-01

    Roč. 93, č. 5 (2016), s. 479-491 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Philometra * Micteroperca * USA Subject RIV: EG - Zoology Impact factor: 1.181, year: 2016

  19. Bathyodontus mirus (Andrássy, 1956, first record of a representative of the suborder Bathyodontina (Nematoda, Mononchida in the Iberian fauna

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    Peña-Santiago, R.

    2010-12-01

    Full Text Available Bathyodontus mirus (Andrássy, 1956 Hopper & Cairns, 1956, collected in sand dunes of SW Iberian peninsula, is studied. Description, measurements and illustrations (LM pictures are provided. Iberian specimens are briefly compared to other known populations of the species. And a compendium of Bathyodontus species, including a key to their identification, is also given. This is the first record of a representative of the nematode suborder Bathyodontina in the Iberian-Balearic range and in the Mediterranean region.Se estudia la especie Bathyodontus mirus (Andrássy, 1956 Hopper y Cairns, 1956, recolectada en dunas de arena en el suroeste peninsular. Se presentan una descripción, medidas e ilustraciones (fotografías con microscopía óptica. Los ejemplares ibéricos se comparan brevemente con otras poblaciones conocidas de la misma especie. Y se ofrece un compendio de las especies del género Bathyodontus, incluida una clave para su identification. Se trata de la primera cita de un miembro del suborden Bathyodontina en el ámbito Ibero-balear y en la región Mediterránea.

  20. Description of .i.Paratylenchus (Gracilacus) straeleni./i. (De Coninck, 1931) Oostenbrink, 1960 (Nematoda: Criconematoidea, Tylenchulidae) from hazelnut in Turkey and its comparison with other world populations

    Czech Academy of Sciences Publication Activity Database

    Akyazi, F.; Felek, A.F.; Čermák, V.; Čudejková, M.; Foit, J.; Yildiz, S.; Háněl, Ladislav

    2015-01-01

    Roč. 52, č. 3 (2015), s. 270-279 ISSN 0440-6605 Grant - others:GA MŠk(CZ) EE2.3.20.0265 Program:EE Institutional support: RVO:60077344 Keywords : Corylus avellana * multivariate analyses * phylogeny * sequencing * species complex Subject RIV: EG - Zoology Impact factor: 0.602, year: 2015

  1. Temporal variation of Mexiconema cichlasomae (Nematoda: Daniconematidae) in the Mayan cichlid fish Cichlasoma urophthalmus and its intermediate host Argulus yucatanus from a tropical coastal lagoon.

    Science.gov (United States)

    May-Tec, A L; Pech, D; Aguirre-Macedo, M L; Lewis, J W; Vidal-Martínez, V M

    2013-03-01

    The aim of the present investigation was to determine whether temporal variation in environmental factors such as rainfall or temperature influence long-term fluctuations in the prevalence and mean abundance of the nematode Mexiconema cichlasomae in the cichlid fish Cichlasoma uropthalmus and its crustacean intermediate host, Argulus yucatanus. The study was undertaken in a tropical coastal lagoon in the Yucatan Peninsula (south-eastern Mexico) over an 8-year period. Variations in temperature, rainfall and monthly infection levels for both hosts were analysed using time series and cross-correlations to detect possible recurrent patterns. Infections of M. cichlasomae in A. yucatanus showed annual peaks, while in C. urophthalmus peaks were bi-annual. The latter appear to be related to the accumulation of several generations of this nematode in C. urophthalmus. Rainfall and temperature appear to be key environmental factors in influencing temporal variation in the infection of M. cichlasomae over periods longer than a year together with the accumulation of larval stages throughout time.

  2. Structural and functional diversity of Nematoda in relation with environmental variables in the Setúbal and Cascais canyons, Western Iberian Margin

    Science.gov (United States)

    Ingels, Jeroen; Billett, David S. M.; Kiriakoulakis, Konstadinos; Wolff, George A.; Vanreusel, Ann

    2011-12-01

    Samples collected at two different depths (ca. 3200 and ca. 4200 m) in the Setúbal and Cascais canyons off the Portuguese coast, during the HERMES RRS Charles Darwin cruise CD179, were analysed for (1) sediment biogeochemistry (TOC, TN) and (2) composition, and structural and trophic diversity of nematode communities. Multivariate PERMANOVA analysis on the nematode community data revealed differences between sediment layers that were greater than differences between canyons, water depths, and stations. This suggests that biogeochemical gradients along the vertical sediment profile are crucial in determining nematode community structure. The interaction between canyon conditions and the nematode community is illustrated by biogeochemical patterns in the sediment and the prevalence of nematode genera that are able to persist in disturbed sediments. Trophic analysis of the nematode community indicated that non-selective deposit feeders are dominant, presumably because of their non-selective feeding behaviour compared to other feeding types, which gives them a competitive advantage in exploiting lower-quality food resources. This study presents a preliminary conceptual scheme for interactions between canyon conditions and the resident fauna.

  3. A new gonad-infecting species of Philometra (Nematoda: Philometridae) from the Atlantic Spanish mackerel Scomberomorus maculatus (Scombridae) off the Atlantic Coast of Florida and South Carolina.

    Science.gov (United States)

    Moravec, František; Bakenhaster, Micah; de Buron, Isaure

    2013-04-01

    A new nematode species, Philometra atlantica n. sp. (Philometridae), is described from male and female specimens found in the ovary of the Atlantic Spanish mackerel, Scomberomorus maculatus (Mitchill) (Scombridae, Perciformes), off the Atlantic coast of Florida and South Carolina. Based on light and scanning electron microscopy examination, the new species differs from most other gonad-infecting Philometra spp. in the length of spicules (111-126 μm), number and arrangement of genital papillae, and a U-shaped, dorsally interrupted caudal mound in the male. A unique feature among all gonad-infecting philometrids is the presence of 2 reflexed dorsal barbs on the distal end of the gubernaculum. From a few congeneric, gonad-infecting species with unknown males, it can be distinguished by some morphological and biometrical features found in gravid females (body length, length of first-stage larvae or esophagus, structure of caudal end) and by the host type (fish family) and geographical distribution. Philometra atlantica is the fourth valid gonad-infecting species of Philometra reported from fishes of the family Scombridae.

  4. Helmintos parasitos das espécies Scomberomorus cavalla (Cuvier e Scomberomorus maculatus (Mitchill do litoral cearense: Contracaecum fortalezae sp. n. (Nematoda, Ascaridoidea

    Directory of Open Access Journals (Sweden)

    Vera Lucia M. Klein

    1973-01-01

    Full Text Available De nematódeos encontrados parasitando estômago, intestinos delgado e grosso de Scomberomorus cavalla (Cuv. e Scomberomorus maculatus (Mitch. o autor propões para o gênero Contracaecum Railliet et Henry, 1912 uma nova espécie, C. fortalezae sp. n., que mais se aproxima de C. clavatum (Rud., 1809 Baylis, 1920 dela se diferenciando por possuir espículos desiguais, um curto ovejetor, útero opistodelfo, ovos menores e por apresentar em todos exemplares estudados ceco intestinal curto e ceco esofagiano longo.In this paper the author proposes a new species of the genus Contracaecum Railliet et Henry, 1912. Of the species under Contracaecum, Contracaecum clavatum (Rudolphi, 1809 Baylis, 1920 is the nearest to Contracaecum fortalezae sp. n. differing from the latter by the following aspects: The males have unequal spicules. The females have a short ovijector and parallel uteri directed backward and the eggs are smaller. In both sexes the intestinal cecum is always short and the ventricular posterior appendix is always long, while Contracaecum clavatum presents these structures in an inverse way, considering the lenght of them. Besides, the thorn-like formations at the posterior end of the body are different.

  5. Philometroides acanthopagri sp. nov., a new philometrid (Nematoda, Philometridae) from the musculature of Acanthopagrus latus (Sparidae) from marine waters of Iraq

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Jassim, A. A. R.; Al-Salim, N. K.

    2012-01-01

    Roč. 57, č. 4 (2012), s. 372-377 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometroides * Acanthopagrus * Iraq Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.000, year: 2012

  6. Two species of Rhabdochona Railliet, 1916 (Nematoda: Rhabdochonidae) parasitising cyprinid fishes in Iraq, with a redescription of R. tigridis Rahemo, 1978 (emend.)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Saraiva, A.; Abdullah, S. M. A.; Bilal, S. J.; Rahemo, Z. I. F.

    2009-01-01

    Roč. 74, č. 2 (2009), s. 125-135 ISSN 0165-5752 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Rhabdochona * Cyprinidae * Iraq Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.911, year: 2009

  7. Two species of Rhabdochona (Nematoda: Rhabdochonidae) from the cyprinid fish Luciobarbus kersin (Heckel) in northern Iraq, including R. (Globochona) kurdistanensis sp. n

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Bilal, S. J.; Abdullah, S. M. A.

    2012-01-01

    Roč. 59, č. 2 (2012), s. 139-147 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Parasitic nematode * Rhabdochona * freshwater fish * Luciobarbus * Iraq Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/pdfs/showpdf.php?pdf=22051

  8. Effect of earthworms (Annelida: Lumbricidae) on the taxonomic composition of Nematoda assemblages in post-mining clays in the Czech Republic - a microcosm experiment

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav

    2010-01-01

    Roč. 74, - (2010), s. 49-54 ISSN 1211-376X. [Central European Workshop on Soil Zoology /10./. České Budějovice, 21.04.2009-24.04.2009] R&D Projects: GA AV ČR 1QS600660505 Institutional research plan: CEZ:AV0Z60660521 Keywords : soil zoology * ecology * diversity Subject RIV: EH - Ecology, Behaviour

  9. Prvé nálezy pôdnych nematódov (Nematoda) pre faunu Slovenska z CHKO Vihorlat

    Czech Academy of Sciences Publication Activity Database

    Háněl, Ladislav; Čerevková, A.

    2010-01-01

    Roč. 15, č. 10 (2010), s. 95-98 ISSN 1335-7522 Grant - others:SAV(SK) 2/0136/10 Institutional research plan: CEZ:AV0Z60660521 Keywords : nematodes * beech forest * Vihorlat Subject RIV: EH - Ecology, Behaviour

  10. Phylogenetic relationships between pinworms (Nematoda: Enterobiinae) parasitising the critically endangered orang-utan, according to the characterisation of molecular genomic and mitochondrial markers

    Czech Academy of Sciences Publication Activity Database

    Foitová, I.; Civáňová, K.; Baruš, Vlastimil; Nurcahyo, W.

    2014-01-01

    Roč. 113, č. 7 (2014), s. 2455-2466 ISSN 0932-0113 Institutional support: RVO:68081766 Keywords : Molecular phylogeny * Cytochromoxidase 1 * 18S rDNA * ITS1 * Orang-utan pinworms * Pongo abelii Subject RIV: EG - Zoology Impact factor: 2.098, year: 2014

  11. Philometra floridensis sp. n. (Nematoda: Philometridae) from the ovary of red drum Sciaenops ocellatus (Osteichthyes: Sciaenidae) off the coast of Florida, USA

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Fajer-Avila, E.; Bakenhaster, M.

    2010-01-01

    Roč. 84, č. 1 (2010), s. 49-54 ISSN 0022-149X R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Sciaenops * Gulf of Mexico Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.544, year: 2010

  12. New philometrids (Nematoda, Philometridae) from head tissues of two serranid fishes (Epinephelus morio and Mycteroperca microlepis) off Florida, northern Gulf of Mexico

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Bakenhaster, M.; Fajer-Avila, E.

    2010-01-01

    Roč. 55, č. 4 (2010), s. 359-368 ISSN 1230-2821 R&D Projects: GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Parasitic nematode * Philometra * marine fish * Epinephelus * Mycteroperca * Gulf of Mexico * Florida Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.144, year: 2010

  13. The life cycle of Huffmanela huffmani Moravec, 1987 (Nematoda: Trichosomoididae), an endemic marine-relict parasite of Centrarchidae from a Central Texas spring

    Czech Academy of Sciences Publication Activity Database

    Worsham, M.L.D.; Huffman, D. G.; Moravec, František; Gibson, J.R.

    2016-01-01

    Roč. 63, JUN 10 (2016), č. článku 020. ISSN 1803-6465 Institutional support: RVO:60077344 Keywords : swim bladder nematode * Trichinelloidea * development * intermediate host * Amphipoda * Hyalella * Gammarus * fish * USA Subject RIV: EG - Zoology Impact factor: 1.082, year: 2016

  14. Redescription of Rhabdochona papuanensis (Nematoda: Thelazioidea), a parasite of rainbow fishes (Melanotaenia spp.); the first record of the species of Rhabdochona in Australia

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Adlard, R.

    2016-01-01

    Roč. 61, č. 4 (2016), s. 820-827 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : Parasitic nematode * Spirurida * freshwater fish * Melanotaeniidae * Queensland * Australian mainland Subject RIV: EG - Zoology Impact factor: 1.160, year: 2016

  15. Redescription of Rhabdochona cotti (Nematoda, Rhabdochonidae) from Cottus caeruleomentum (Teleostei, Cottidae) in Maryland, USA, with remarks on the taxonomy of North American Rhabdochona spp

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Muzzall, P.

    2007-01-01

    Roč. 52, č. 1 (2007), s. 51-57 ISSN 1230-2821 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : Rhabdochona * Cottus * USA Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 0.814, year: 2007

  16. Dentirumai philippinensis n. gen., n. sp (Nematoda: Philometridae), a new tissue-infecting philometrid nematode from the loach goby Rhyacichthys aspro (Valenciennes) (Rhyacichthyidae) in the Philippines

    Czech Academy of Sciences Publication Activity Database

    Quiazon, K.M.A.; Moravec, František

    2013-01-01

    Roč. 87, č. 1 (2013), s. 59-65 ISSN 0022-149X R&D Projects: GA MŠk LC522 Institutional support: RVO:60077344 Keywords : Philometridae * Phillipine Archipelago * Parasitic nematode Subject RIV: EA - Cell Biology Impact factor: 1.303, year: 2013

  17. Spatial distribution of the nematodes in the subtidal community of the Central West Coast of India with emphasis on Tershellingia longicaudata (Nematoda: Linhomoeidae)

    Digital Repository Service at National Institute of Oceanography (India)

    Nanajkar, M.; Ingole, B.S.; Chatterjee, T.

    , Blaxter M. 2001. Systematic position and phylogeny. In Biology of Nematodes ed. DL Lee, (London: Harwood Academic Publishers), pp.1-30. El Wakeel SK, Riley JP. 1957. The determination of organic carbon in marine muds. Journal du Conseil Permanent... determination of chlorophyll. Journal du Conseil Permanent International Pourl Exploration de la mer. 30:3-15. Ingole B, Sivadas S, Goltekar R, Clemente S, Nanajkar M, Sawant R, D’Silva C, Sarkar A, Ansari Z. 2006. Ecotoxicological effect of grounded MV River...

  18. New data on the morphology of Iheringascaris inquies (Linton, 1901) (Nematoda: Anisakidae), a specific parasite of the marine fish Rachycentron canadum (Linnaeus), as revealed by SEM

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Yost, J.; de Buron, I.

    2012-01-01

    Roč. 59, č. 4 (2012), s. 315-318 ISSN 0015-5683 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : fish host * marine fish * morphology * North America * parasitic nematode Subject RIV: EA - Cell Biology Impact factor: 2.515, year: 2012 http://folia.paru.cas.cz/pdfs/showpdf.php?pdf=22098

  19. A new species of Philometra Costa, 1845 (Nematoda: Philometridae) from the freshwater fish (red piranha) Pygocentrus nattereri Kner (Characidae) in Amazonia, Brazil

    Czech Academy of Sciences Publication Activity Database

    Cárdenas, M. Q.; Moravec, František; Fernandes, B. M. M.; Morais, A. M.

    2012-01-01

    Roč. 83, č. 2 (2012), s. 137-144 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Pygocentrus * Brazil Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.260, year: 2012 http://link.springer.com/article/10.1007%2Fs11230-012-9377-4

  20. Female morphology of Philometra hyderabadensis Rasheed, 1963 (Nematoda: Philometridae), a little-known parasite of the catfish Wallago attu Bloch & Schneider in India, as revealed by SEM observations

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Chavan, S. P.

    2012-01-01

    Roč. 83, č. 2 (2012), s. 117-122 ISSN 0165-5752 R&D Projects: GA ČR GBP505/12/G112 Institutional research plan: CEZ:AV0Z60220518 Keywords : Philometra * Wallago * India Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.260, year: 2012