Glycolytic overload and the genesis of breast cancer.

Science.gov (United States)

Robson, J R

1984-03-01

It is suggested that the development of breast cancer is due to overloading of the glycolytic pathways. An excess of substrates or an excessive delivery rate of substrates to the Krebs Cycle is believed to result in the formation of acetyl CoA. Feedback mechanisms controlling the conversion of acetyl CoA to cholesterol may be overcome; the resulting high concentration of cholesterol induces the formation of pregnenolone which may then be converted into androgens, estrogens and progesterone. These steroids are in addition to those produced by gonads and adrenal glands. Glycolytic overload is also associated with an increase in fat stores which have been shown to be the site of interconversion of sex hormones. Excess sex hormones or abnormal sex hormones are believed to be the cause of breast cancer. The hypothesis presented links glycolytic overload with clinical biochemical phenomena and explains some of the anomalies observed in breast cancer experience in different ethnic groups. Changes in dietary habits during the history of man resulting in " gorging " and the consumption of highly refined sugars are possible causes of glycolytic overload. So, also, is impaired thermogenesis due to Brown Fat deficits in certain ethnic groups.

Concentration and flux of total and dissolved phosphorus, total nitrogen, chloride, and total suspended solids for monitored tributaries of Lake Champlain, 1990-2012

Science.gov (United States)

Medalie, Laura

2014-01-01

Annual and daily concentrations and fluxes of total and dissolved phosphorus, total nitrogen, chloride, and total suspended solids were estimated for 18 monitored tributaries to Lake Champlain by using the Weighted Regressions on Time, Discharge, and Seasons regression model. Estimates were made for 21 or 23 years, depending on data availability, for the purpose of providing timely and accessible summary reports as stipulated in the 2010 update to the Lake Champlain “Opportunities for Action” management plan. Estimates of concentration and flux were provided for each tributary based on (1) observed daily discharges and (2) a flow-normalizing procedure, which removed the random fluctuations of climate-related variability. The flux bias statistic, an indicator of the ability of the Weighted Regressions on Time, Discharge, and Season regression models to provide accurate representations of flux, showed acceptable bias (less than ±10 percent) for 68 out of 72 models for total and dissolved phosphorus, total nitrogen, and chloride. Six out of 18 models for total suspended solids had moderate bias (between 10 and 30 percent), an expected result given the frequently nonlinear relation between total suspended solids and discharge. One model for total suspended solids with a very high bias was influenced by a single extreme value; however, removal of that value, although reducing the bias substantially, had little effect on annual fluxes.

Total hydrogen and oxygen fluxes in the edge plasma of tokamaks

International Nuclear Information System (INIS)

Kastelewicz, H.

1988-01-01

A relativistic model of the edge plasma of tokamaks is described considering the primary neutral fluxes emitted from limiter and wall. The primary neutrals, which determine essentially the particle flux balance in the plasma edge, the scrape-off layer plasma and the particles adsorbed at limiter and wall are treated as separate subsystems which are iteratively coupled through the mutual particle sinks and sources. The model is used for the calculation of total hydrogen and oxygen fluxes in edge plasma of tokamaks. The results for different fractions of and contributions to the total fluxes are illustrated and discussed

Role of Glycolytic Intermediates in Global Regulation and Signal Transduction. Final Report

Energy Technology Data Exchange (ETDEWEB)

Liao, J.C.

2000-05-08

The goal of this project is to determine the role of glycolytic intermediates in regulation of cell physiology. It is known that many glycolytic intermediates are involved in regulation of enzyme activities at the kinetic level. However, little is known regarding the role of these metabolites in global regulation and signal transduction. This project aims to investigate the role of glycolytic intermediates in the regulation of gene expression.

Transcriptional responses to glucose at different glycolytic rates in Saccharomyces cerevisiae.

Science.gov (United States)

Elbing, Karin; Ståhlberg, Anders; Hohmann, Stefan; Gustafsson, Lena

2004-12-01

The addition of glucose to Saccharomyces cerevisiae cells causes reprogramming of gene expression. Glucose is sensed by membrane receptors as well as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Rapid glucose responses were observed in all strains able to take up glucose, consistent with intracellular sensing. The degree of long-term responses, however, clearly correlated with the glycolytic rate: glucose-stimulated expression of genes encoding enzymes of the lower part of glycolysis showed an almost linear correlation with the glycolytic rate, while expression levels of genes encoding gluconeogenic enzymes and invertase (SUC2) showed an inverse correlation. Glucose control of SUC2 expression is mediated by the Snf1-Mig1 pathway. Mig1 dephosphorylation upon glucose addition is known to lead to repression of target genes. Mig1 was initially dephosphorylated upon glucose addition in all strains able to take up glucose, but remained dephosphorylated only at high glycolytic rates. Remarkably, transient Mig1-dephosphorylation was accompanied by the repression of SUC2 expression at high glycolytic rates, but stimulated SUC2 expression at low glycolytic rates. This suggests that Mig1-mediated repression can be overruled by factors mediating induction via a low glucose signal. At low and moderate glycolytic rates, Mig1 was partly dephosphorylated both in the presence of phosphorylated, active Snf1, and unphosphorylated, inactive Snf1, indicating that Mig1 was actively phosphorylated and dephosphorylated simultaneously, suggesting independent control of both processes. Taken together, it appears that glucose addition affects the expression of SUC2 as well as Mig1 activity by both Snf1-dependent and -independent mechanisms that can now be dissected and resolved as early and late/sustained responses.

Hyperthyroidism results in increased glycolytic capacity in the rat heart. A 31P-NMR study.

Science.gov (United States)

Seymour, A M; Eldar, H; Radda, G K

1990-11-12

We have investigated the metabolic adaptations that occur in the thyroxine-treated rat heart. Rats were made hyperthyroid by daily intra-peritoneal injections of thyroxine (35 micrograms/100 g body weight) over seven days. 31P-NMR investigations of isolated glucose-perfused isometric hearts showed that thyroxine treatment caused an increase in Pi (from 4.9 mumols.(g dry wt.)-1 in control hearts to 11.7 mumols.(g dry wt.)-1 in hyperthyroid hearts), a decrease in phosphocreatine (from 36.5 mumols.(g dry wt.)-1 to 21.8 mumols.(g dry wt.)-1) with no change in ATP or ADP concentrations under the same conditions of cardiac work. The unidirectional exchange flux Pi----ATP was measured by saturation transfer NMR in hyperthyroid rat hearts. This exchange (which has been shown to contain a significant glycolytic component) increased by 2.2-fold in thyroxine-treated hearts in comparison to control hearts (to 3.6 mumols.(g dry wt.)-1.s-1, from 1.6 mumols.(g dry wt.)-1.s-1). In parallel experiments, NMR analysis of extracts from hyperthyroid rat hearts showed significantly elevated levels of glucose 6-phosphate, and fructose 6-phosphate. Measurements of enzyme activities isolated from hyperthyroid and control tissue showed a 40% increase in phosphofructokinase activity. These data together with the increased concentration of Pi show that both glycolytic and glycogenolytic fluxes are increased in the hyperthyroid rat heart. This metabolic adaptation may be necessary to cope with the increased number and activity of Na+/K(+)-ATPase pumps that occur in response to thyroxine treatment.

A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin.

Science.gov (United States)

Araiza-Olivera, Daniela; Chiquete-Felix, Natalia; Rosas-Lemus, Mónica; Sampedro, José G; Peña, Antonio; Mujica, Adela; Uribe-Carvajal, Salvador

2013-08-01

In the Saccharomyces cerevisiae glycolytic pathway, 11 enzymes catalyze the stepwise conversion of glucose to two molecules of ethanol plus two CO₂ molecules. In the highly crowded cytoplasm, this pathway would be very inefficient if it were dependent on substrate/enzyme diffusion. Therefore, the existence of a multi-enzymatic glycolytic complex has been suggested. This complex probably uses the cytoskeleton to stabilize the interaction of the various enzymes. Here, the role of filamentous actin (F-actin) in stabilization of a putative glycolytic metabolon is reported. Experiments were performed in isolated enzyme/actin mixtures, cytoplasmic extracts and permeabilized yeast cells. Polymerization of actin was promoted using phalloidin or inhibited using cytochalasin D or latrunculin. The polymeric filamentous F-actin, but not the monomeric globular G-actin, stabilized both the interaction of isolated glycolytic pathway enzyme mixtures and the whole fermentation pathway, leading to higher fermentation activity. The associated complexes were resistant against inhibition as a result of viscosity (promoted by the disaccharide trehalose) or inactivation (using specific enzyme antibodies). In S. cerevisiae, a glycolytic metabolon appear to assemble in association with F-actin. In this complex, fermentation activity is enhanced and enzymes are partially protected against inhibition by trehalose or by antibodies. © 2013 FEBS.

Study of AMPK-Regulated Metabolic Fluxes in Neurons Using the Seahorse XFe Analyzer.

Science.gov (United States)

Marinangeli, Claudia; Kluza, Jérome; Marchetti, Philippe; Buée, Luc; Vingtdeux, Valérie

2018-01-01

AMP-activated protein kinase (AMPK) is the intracellular master energy sensor and metabolic regulator. AMPK is involved in cell energy homeostasis through the regulation of glycolytic flux and mitochondrial biogenesis. Interestingly, metabolic dysfunctions and AMPK deregulations are observed in many neurodegenerative diseases, including Alzheimer's. While these deregulations could play a key role in the development of these diseases, the study of metabolic fluxes has remained quite challenging and time-consuming. In this chapter, we describe the Seahorse XFe respirometry assay as a fundamental experimental tool to investigate the role of AMPK in controlling and modulating cell metabolic fluxes in living and intact differentiated primary neurons. The Seahorse XFe respirometry assay allows the real-time monitoring of glycolytic flux and mitochondrial respiration from different kind of cells, tissues, and isolated mitochondria. Here, we specify a protocol optimized for primary neuronal cells using several energy substrates such as glucose, pyruvate, lactate, glutamine, and ketone bodies. Nevertheless, this protocol can easily be adapted to monitor metabolic fluxes from other types of cells, tissues, or isolated mitochondria by taking into account the notes proposed for each key step of this assay.

Inhibition of Non-flux-Controlling Enzymes Deters Cancer Glycolysis by Accumulation of Regulatory Metabolites of Controlling Steps.

Science.gov (United States)

Marín-Hernández, Álvaro; Rodríguez-Zavala, José S; Del Mazo-Monsalvo, Isis; Rodríguez-Enríquez, Sara; Moreno-Sánchez, Rafael; Saavedra, Emma

2016-01-01

Glycolysis provides precursors for the synthesis of macromolecules and may contribute to the ATP supply required for the constant and accelerated cellular duplication in cancer cells. In consequence, inhibition of glycolysis has been reiteratively considered as an anti-cancer therapeutic option. In previous studies, kinetic modeling of glycolysis in cancer cells allowed the identification of the main steps that control the glycolytic flux: glucose transporter, hexokinase (HK), hexose phosphate isomerase (HPI), and glycogen degradation in human cervix HeLa cancer cells and rat AS-30D ascites hepatocarcinoma. It was also previously experimentally determined that simultaneous inhibition of the non-controlling enzymes lactate dehydrogenase (LDH), pyruvate kinase (PYK), and enolase (ENO) brings about significant decrease in the glycolytic flux of cancer cells and accumulation of intermediate metabolites, mainly fructose-1,6-bisphosphate (Fru1,6BP), and dihydroxyacetone phosphate (DHAP), which are inhibitors of HK and HPI, respectively. Here it was found by kinetic modeling that inhibition of cancer glycolysis can be attained by blocking downstream non flux-controlling steps as long as Fru1,6BP and DHAP, regulatory metabolites of flux-controlling enzymes, are accumulated. Furthermore, experimental results and further modeling showed that oxamate and iodoacetate inhibitions of PYK, ENO, and glyceraldehyde3-phosphate dehydrogenase (GAPDH), but not of LDH and phosphoglycerate kinase, induced accumulation of Fru1,6BP and DHAP in AS-30D hepatoma cells. Indeed, PYK, ENO, and GAPDH exerted the highest control on the Fru1,6BP and DHAP concentrations. The high levels of these metabolites inhibited HK and HPI and led to glycolytic flux inhibition, ATP diminution, and accumulation of toxic methylglyoxal. Hence, the anticancer effects of downstream glycolytic inhibitors are very likely mediated by this mechanism. In parallel, it was also found that uncompetitive inhibition of the

LeftyA sensitive cytosolic pH regulation and glycolytic flux in Ishikawa human endometrial cancer cells

Energy Technology Data Exchange (ETDEWEB)

Salker, Madhuri S.; Zhou, Yuetao; Singh, Yogesh [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany); Brosens, Jan [Division of Reproductive Health, Warwick Medical School, Clinical Sciences Research Laboratories, University Hospital, Coventry CV2 2DX (United Kingdom); Lang, Florian, E-mail: florian.lang@uni-tuebingen.de [Department of Physiology, University of Tuebingen, 72076 Tuebingen (Germany)

2015-05-08

Objective: LeftyA, a powerful regulator of stemness, embryonic differentiation, and reprogramming of cancer cells, counteracts cell proliferation and tumor growth. Key properties of tumor cells include enhanced glycolytic flux, which is highly sensitive to cytosolic pH and thus requires export of H{sup +} and lactate. H{sup +} extrusion is in part accomplished by Na{sup +}/H{sup +} exchangers, such as NHE1. An effect of LeftyA on transport processes has, however, never been reported. The present study thus explored whether LeftyA modifies regulation of cytosolic pH (pHi) in Ishikawa cells, a well differentiated endometrial carcinoma cell model. Methods: NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance quantified by Western blotting, pH{sub i} estimated utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na{sup +}/H{sup +} exchanger activity from Na{sup +} dependent realkalinization after an ammonium pulse, and lactate concentration in the supernatant utilizing an enzymatic assay and subsequent colorimetry. Results: A 2 h treatment with LeftyA (8 ng/ml) significantly decreased NHE1 transcript levels (by 99.6%), NHE1 protein abundance (by 71%), Na{sup +}/H{sup +} exchanger activity (by 55%), pHi (from 7.22 ± 0.02 to 7.05 ± 0.02), and lactate release (by 41%). Conclusions: LeftyA markedly down-regulates NHE1 expression, Na{sup +}/H{sup +} exchanger activity, pHi, and lactate release in Ishikawa cells. Those effects presumably contribute to cellular reprogramming and growth inhibition. - Highlights: • LeftyA, an inhibitor of tumor growth, reduces Na{sup +}/H{sup +}-exchanger activity by 55%. • LeftyA decreases NHE1 transcripts by 99.6% and NHE1 protein by 71%. • LeftyA decreases cytosolic pH from 7.22 ± 0.02 to 7.05 ± 0.02. • Cytosolic acidification by Lefty A decreases glycolysis by 41%. • Cytosolic acidification by Lefty A compromises energy production of tumor cells.

Nonlinear correlations in the hydrophobicity and average flexibility along the glycolytic enzymes sequences

Energy Technology Data Exchange (ETDEWEB)

Ciorsac, Alecu, E-mail: aleciorsac@yahoo.co [Politehnica University of Timisoara, Department of Physical Education and Sport, 2 P-ta Victoriei, 300006, Timisoara (Romania); Craciun, Dana, E-mail: craciundana@gmail.co [Teacher Training Department, West University of Timisoara, 4 Boulevard V. Pirvan, Timisoara, 300223 (Romania); Ostafe, Vasile, E-mail: vostafe@cbg.uvt.r [Department of Chemistry, West University of Timisoara, 16 Pestallozi, 300115, Timisoara (Romania); Laboratory of Advanced Researches in Environmental Protection, Nicholas Georgescu-Roegen Interdisciplinary Research and Formation Platform, 4 Oituz, Timisoara, 300086 (Romania); Isvoran, Adriana, E-mail: aisvoran@cbg.uvt.r [Department of Chemistry, West University of Timisoara, 16 Pestallozi, 300115, Timisoara (Romania); Laboratory of Advanced Researches in Environmental Protection, Nicholas Georgescu-Roegen Interdisciplinary Research and Formation Platform, 4 Oituz, Timisoara, 300086 (Romania)

2011-04-15

Research highlights: lights: We focus our study on the glycolytic enzymes. We reveal correlation of hydrophobicity and flexibility along their chains. We also reveal fractal aspects of the glycolytic enzymes structures and surfaces. The glycolytic enzyme sequences are not random. Creation of fractal structures requires the operation of nonlinear dynamics. - Abstract: Nonlinear methods widely used for time series analysis were applied to glycolytic enzyme sequences to derive information concerning the correlation of hydrophobicity and average flexibility along their chains. The 20 sequences of different types of the 10 human glycolytic enzymes were considered as spatial series and were analyzed by spectral analysis, detrended fluctuations analysis and Hurst coefficient calculation. The results agreed that there are both short range and long range correlations of hydrophobicity and average flexibility within investigated sequences, the short range correlations being stronger and indicating that local interactions are the most important for the protein folding. This correlation is also reflected by the fractal nature of the structures of investigated proteins.

Nonlinear correlations in the hydrophobicity and average flexibility along the glycolytic enzymes sequences

International Nuclear Information System (INIS)

Ciorsac, Alecu; Craciun, Dana; Ostafe, Vasile; Isvoran, Adriana

2011-01-01

Research highlights: → We focus our study on the glycolytic enzymes. → We reveal correlation of hydrophobicity and flexibility along their chains. → We also reveal fractal aspects of the glycolytic enzymes structures and surfaces. → The glycolytic enzyme sequences are not random. → Creation of fractal structures requires the operation of nonlinear dynamics. - Abstract: Nonlinear methods widely used for time series analysis were applied to glycolytic enzyme sequences to derive information concerning the correlation of hydrophobicity and average flexibility along their chains. The 20 sequences of different types of the 10 human glycolytic enzymes were considered as spatial series and were analyzed by spectral analysis, detrended fluctuations analysis and Hurst coefficient calculation. The results agreed that there are both short range and long range correlations of hydrophobicity and average flexibility within investigated sequences, the short range correlations being stronger and indicating that local interactions are the most important for the protein folding. This correlation is also reflected by the fractal nature of the structures of investigated proteins.

Primary Metabolic Pathways and Metabolic Flux Analysis

DEFF Research Database (Denmark)

Villadsen, John

2015-01-01

his chapter introduces the metabolic flux analysis (MFA) or stoichiometry-based MFA, and describes the quantitative basis for MFA. It discusses the catabolic pathways in which free energy is produced to drive the cell-building anabolic pathways. An overview of these primary pathways provides...... the reader who is primarily trained in the engineering sciences with atleast a preliminary introduction to biochemistry and also shows how carbon is drained off the catabolic pathways to provide precursors for cell mass building and sometimes for important industrial products. The primary pathways...... to be examined in the following are: glycolysis, primarily by the EMP pathway, but other glycolytic pathways is also mentioned; fermentative pathways in which the redox generated in the glycolytic reactions are consumed; reactions in the tricarboxylic acid (TCA) cycle, which produce biomass precursors and redox...

31P magnetization transfer measurements of Pi→ATP flux in exercising human muscle.

Science.gov (United States)

Sleigh, Alison; Savage, David B; Williams, Guy B; Porter, David; Carpenter, T Adrian; Brindle, Kevin M; Kemp, Graham J

2016-03-15

Fundamental criticisms have been made over the use of (31)P magnetic resonance spectroscopy (MRS) magnetization transfer estimates of inorganic phosphate (Pi)→ATP flux (VPi-ATP) in human resting skeletal muscle for assessing mitochondrial function. Although the discrepancy in the magnitude of VPi-ATP is now acknowledged, little is known about its metabolic determinants. Here we use a novel protocol to measure VPi-ATP in human exercising muscle for the first time. Steady-state VPi-ATP was measured at rest and over a range of exercise intensities and compared with suprabasal oxidative ATP synthesis rates estimated from the initial rates of postexercise phosphocreatine resynthesis (VATP). We define a surplus Pi→ATP flux as the difference between VPi-ATP and VATP. The coupled reactions catalyzed by the glycolytic enzymes GAPDH and phosphoglycerate kinase (PGK) have been shown to catalyze measurable exchange between ATP and Pi in some systems and have been suggested to be responsible for this surplus flux. Surplus VPi-ATP did not change between rest and exercise, even though the concentrations of Pi and ADP, which are substrates for GAPDH and PGK, respectively, increased as expected. However, involvement of these enzymes is suggested by correlations between absolute and surplus Pi→ATP flux, both at rest and during exercise, and the intensity of the phosphomonoester peak in the (31)P NMR spectrum. This peak includes contributions from sugar phosphates in the glycolytic pathway, and changes in its intensity may indicate changes in downstream glycolytic intermediates, including 3-phosphoglycerate, which has been shown to influence the exchange between ATP and Pi catalyzed by GAPDH and PGK. Copyright © 2016 the American Physiological Society.

Does SW Monsoon Influence Total Suspended Matter Flux into the Arabian Sea?

Digital Repository Service at National Institute of Oceanography (India)

Raghavan, B.R.; Chauhan, O.S.

Seasonal enhancement in the flux of total suspended matter (TSM) has been attributed to climatology of the SW monsoon (SWM) in time-series trap experiments conducted in the Arabian Sea. To determine the influence of climate on TSM flux, synoptic...

Primordial oscillations in life: Direct observation of glycolytic oscillations in individual HeLa cervical cancer cells

Science.gov (United States)

Amemiya, Takashi; Shibata, Kenichi; Itoh, Yoshihiro; Itoh, Kiminori; Watanabe, Masatoshi; Yamaguchi, Tomohiko

2017-10-01

We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future.

Sodium bicarbonate ingestion increases glycolytic contribution and improves performance during simulated taekwondo combat.

Science.gov (United States)

Lopes-Silva, João Paulo; Da Silva Santos, Jonatas Ferreira; Artioli, Guilherme Giannini; Loturco, Irineu; Abbiss, Chris; Franchini, Emerson

2018-04-01

To investigate the effect of sodium bicarbonate (NaHCO 3 ) on performance and estimated energy system contribution during simulated taekwondo combat. Nine taekwondo athletes completed two experimental sessions separated by at least 48 h. Athletes consumed 300 mg/kg body mass of NaHCO 3 or placebo (CaCO 3 ) 90 min before the combat simulation (three rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration [La - ] and rating of perceived exertion (RPE) were measured before and after each round, whereas heart rate (HR) and the estimated contribution of the oxidative (W OXI ), ATP (adenosine triphosphate)-phosphocreatine (PCr) (W PCR ), and glycolytic (W [ La - ] ) systems were calculated during the combat simulation. [La - ] increased significantly after NaHCO 3 ingestion, when compared with the placebo condition (+14%, P = 0.04, d = 3.70). NaHCO 3 ingestion resulted in greater estimated glycolytic energy contribution in the first round when compared with the placebo condition (+31%, P = 0.01, d = 3.48). Total attack time was significantly greater after NaHCO 3 when compared with placebo (+13%, P = 0.05, d = 1.15). W OXI , W PCR , VO 2 , HR and RPE were not different between conditions (P > 0.05). NaHCO 3 ingestion was able to increase the contribution of glycolytic metabolism and, therefore, improve performance during simulated taekwondo combat.

The glycolytic pathway of Trimastix pyriformis is an evolutionary mosaic

Directory of Open Access Journals (Sweden)

Silberman Jeffrey D

2006-11-01

Full Text Available Abstract Background Glycolysis and subsequent fermentation is the main energy source for many anaerobic organisms. The glycolytic pathway consists of ten enzymatic steps which appear to be universal amongst eukaryotes. However, it has been shown that the origins of these enzymes in specific eukaryote lineages can differ, and sometimes involve lateral gene transfer events. We have conducted an expressed sequence tag (EST survey of the anaerobic flagellate Trimastix pyriformis to investigate the nature of the evolutionary origins of the glycolytic enzymes in this relatively unstudied organism. Results We have found genes in the Trimastix EST data that encode enzymes potentially catalyzing nine of the ten steps of the glycolytic conversion of glucose to pyruvate. Furthermore, we have found two different enzymes that in principle could catalyze the conversion of phosphoenol pyruvate (PEP to pyruvate (or the reverse reaction as part of the last step in glycolysis. Our phylogenetic analyses of all of these enzymes revealed at least four cases where the relationship of the Trimastix genes to homologs from other species is at odds with accepted organismal relationships. Although lateral gene transfer events likely account for these anomalies, with the data at hand we were not able to establish with confidence the bacterial donor lineage that gave rise to the respective Trimastix enzymes. Conclusion A number of the glycolytic enzymes of Trimastix have been transferred laterally from bacteria instead of being inherited from the last common eukaryotic ancestor. Thus, despite widespread conservation of the glycolytic biochemical pathway across eukaryote diversity, in a number of protist lineages the enzymatic components of the pathway have been replaced by lateral gene transfer from disparate evolutionary sources. It remains unclear if these replacements result from selectively advantageous properties of the introduced enzymes or if they are neutral

The unique features of glycolytic pathways in Archaea.

Science.gov (United States)

Verhees, Corné H; Kengen, Servé W M; Tuininga, Judith E; Schut, Gerrit J; Adams, Michael W W; De Vos, Willem M; Van Der Oost, John

2003-10-15

An early divergence in evolution has resulted in two prokaryotic domains, the Bacteria and the Archaea. Whereas the central metabolic routes of bacteria and eukaryotes are generally well-conserved, variant pathways have developed in Archaea involving several novel enzymes with a distinct control. A spectacular example of convergent evolution concerns the glucose-degrading pathways of saccharolytic archaea. The identification, characterization and comparison of the glycolytic enzymes of a variety of phylogenetic lineages have revealed a mosaic of canonical and novel enzymes in the archaeal variants of the Embden-Meyerhof and the Entner-Doudoroff pathways. By means of integrating results from biochemical and genetic studies with recently obtained comparative and functional genomics data, the structure and function of the archaeal glycolytic routes, the participating enzymes and their regulation are re-evaluated.

Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells.

Science.gov (United States)

Abdelazeem, Khalid N M; Singh, Yogesh; Lang, Florian; Salker, Madhuri S

2017-01-01

Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by Na+/H+ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of Ellagic acid on transport processes has, however, never been reported. The present study thus elucidated an effect of Ellagic acid on cytosolic pH (pHi), NHE1 transcript levels, NHE1 protein abundance, Na+/H+ exchanger activity, and lactate release. Experiments were performed in Ishikawa cells without or with prior Ellagic acid (20 µM) treatment. NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance by Western blotting, pHi utilizing (2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein [BCECF] fluorescence, Na+/H+ exchanger activity from Na+ dependent realkalinization after an ammonium pulse, cell volume from forward scatter in flow cytometry, reactive oxygen species (ROS) from 2',7'-dichlorodihydrofluorescein fluorescence, glucose uptake utilizing 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose, and lactate concentration in the supernatant utilizing a colorimetric (570 nm)/ fluorometric enzymatic assay. A 48 hour treatment with Ellagic acid (20 µM) significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 ± 0.01 to 7.02 ± 0.01, Na+/H+ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Ellagic acid (20µM) markedly down-regulates ROS formation and NHE1 expression leading to decreased Na+/H+ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Those effects presumably contribute to reprogramming and growth

Negative Effect of Ellagic Acid on Cytosolic pH Regulation and Glycolytic Flux in Human Endometrial Cancer Cells

Directory of Open Access Journals (Sweden)

Khalid N. M. Abdelazeem

2017-04-01

Full Text Available Background/Aims: Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by Na+/H+ exchangers, such as NHE1. The carrier is sensitive to oxidative stress. Growth of tumor cells could be suppressed by the polyphenol Ellagic acid, which is found in various fruits and vegetables. An effect of Ellagic acid on transport processes has, however, never been reported. The present study thus elucidated an effect of Ellagic acid on cytosolic pH (pHi, NHE1 transcript levels, NHE1 protein abundance, Na+/H+ exchanger activity, and lactate release. Methods: Experiments were performed in Ishikawa cells without or with prior Ellagic acid (20 µM treatment. NHE1 transcript levels were determined by qRT-PCR, NHE1 protein abundance by Western blotting, pHi utilizing (2',7'-bis-(2-carboxyethyl-5-(and-6-carboxyfluorescein [BCECF] fluorescence, Na+/H+ exchanger activity from Na+ dependent realkalinization after an ammonium pulse, cell volume from forward scatter in flow cytometry, reactive oxygen species (ROS from 2’,7’-dichlorodihydrofluorescein fluorescence, glucose uptake utilizing 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-ylamino-2-deoxyglucose, and lactate concentration in the supernatant utilizing a colorimetric (570 nm/ fluorometric enzymatic assay. Results: A 48 hour treatment with Ellagic acid (20 µM significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 ± 0.01 to 7.02 ± 0.01, Na+/H+ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Conclusion: Ellagic acid (20µM markedly down-regulates ROS formation and NHE1 expression leading to decreased Na+/H+ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Those

Exchange of reactive nitrogen compounds: concentrations and fluxes of total ammonium and total nitrate above a spruce canopy

Directory of Open Access Journals (Sweden)

V. Wolff

2010-05-01

Full Text Available Total ammonium (tot-NH₄⁺ and total nitrate (tot-NO₃⁻ provide chemically conservative quantities in the measurement of surface exchange of reactive nitrogen compounds ammonia (NH₃, particulate ammonium (NH₄⁺, nitric acid (HNO₃, and particulate nitrate (NO₃⁻, using the aerodynamic gradient method. Total fluxes were derived from concentration differences of total ammonium (NH₃ and NH₄⁺ and total nitrate (HNO₃ and NO₃⁻ measured at two levels. Gaseous species and related particulate compounds were measured selectively, simultaneously and continuously above a spruce forest canopy in south-eastern Germany in summer 2007. Measurements were performed using a wet-chemical two-point gradient instrument, the GRAEGOR. Median concentrations of NH₃, HNO₃, NH₄⁺, and NO₃⁻ were 0.57, 0.12, 0.76, and 0.48 μg m⁻³, respectively. Total ammonium and total nitrate fluxes showed large variations depending on meteorological conditions, with concentrations close to zero under humid and cool conditions and higher concentrations under dry conditions. Mean fluxes of total ammonium and total nitrate in September 2007 were directed towards the forest canopy and were −65.77 ng m⁻² s⁻¹ and −41.02 ng m⁻² s⁻¹ (in terms of nitrogen, respectively. Their deposition was controlled by aerodynamic resistances only, with very little influence of surface resistances. Including measurements of wet deposition and findings of former studies on occult deposition (fog water interception at the study site, the total N deposition in September 2007 was estimated to 5.86 kg ha⁻¹.

Extracellular functions of glycolytic enzymes of parasites: unpredicted use of ancient proteins.

Science.gov (United States)

Gómez-Arreaza, Amaranta; Acosta, Hector; Quiñones, Wilfredo; Concepción, Juan Luis; Michels, Paul A M; Avilán, Luisana

2014-02-01

In addition of their usual intracellular localization where they are involved in catalyzing reactions of carbohydrate and energy metabolism by glycolysis, multiple studies have shown that glycolytic enzymes of many organisms, but notably pathogens, can also be present extracellularly. In the case of parasitic protists and helminths, they can be found either secreted or attached to the surface of the parasites. At these extracellular localizations, these enzymes have been shown to perform additional, very different so-called "moonlighting" functions, such as acting as ligands for a variety of components of the host. Due to this recognition, different extracellular glycolytic enzymes participate in various important parasite-host interactions such as adherence and invasion of parasites, modulation of the host's immune and haemostatic systems, promotion of angiogenesis, and acquisition of specific nutrients by the parasites. Accordingly, extracellular glycolytic enzymes are important for the invasion of the parasites and their establishment in the host, and in determining their virulence. Copyright © 2014 Elsevier B.V. All rights reserved.

Programmed mitophagy is essential for the glycolytic switch during cell differentiation.

Science.gov (United States)

Esteban-Martínez, Lorena; Sierra-Filardi, Elena; McGreal, Rebecca S; Salazar-Roa, María; Mariño, Guillermo; Seco, Esther; Durand, Sylvère; Enot, David; Graña, Osvaldo; Malumbres, Marcos; Cvekl, Ales; Cuervo, Ana María; Kroemer, Guido; Boya, Patricia

2017-06-14

Retinal ganglion cells (RGCs) are the sole projecting neurons of the retina and their axons form the optic nerve. Here, we show that embryogenesis-associated mouse RGC differentiation depends on mitophagy, the programmed autophagic clearance of mitochondria. The elimination of mitochondria during RGC differentiation was coupled to a metabolic shift with increased lactate production and elevated expression of glycolytic enzymes at the mRNA level. Pharmacological and genetic inhibition of either mitophagy or glycolysis consistently inhibited RGC differentiation. Local hypoxia triggered expression of the mitophagy regulator BCL2/adenovirus E1B 19-kDa-interacting protein 3-like (BNIP3L, best known as NIX) at peak RGC differentiation. Retinas from NIX-deficient mice displayed increased mitochondrial mass, reduced expression of glycolytic enzymes and decreased neuronal differentiation. Similarly, we provide evidence that NIX-dependent mitophagy contributes to mitochondrial elimination during macrophage polarization towards the proinflammatory and more glycolytic M1 phenotype, but not to M2 macrophage differentiation, which primarily relies on oxidative phosphorylation. In summary, developmentally controlled mitophagy promotes a metabolic switch towards glycolysis, which in turn contributes to cellular differentiation in several distinct developmental contexts. © 2017 The Authors.

Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle

Science.gov (United States)

2017-01-01

Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose), completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-‘ve values = H+ release) for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate), and -1.96 and -0.01 (lactate), respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and –0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non-mitochondrial energy

Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle.

Science.gov (United States)

Robergs, Robert Andrew

2017-01-01

Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose), completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-'ve values = H+ release) for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate), and -1.96 and -0.01 (lactate), respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and -0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non-mitochondrial energy

Competitive cation binding computations of proton balance for reactions of the phosphagen and glycolytic energy systems within skeletal muscle.

Directory of Open Access Journals (Sweden)

Robert Andrew Robergs

Full Text Available Limited research and data has been published for the H+ coefficients for the metabolites and reactions involved in non-mitochondrial energy metabolism. The purpose of this investigation was to compute the fractional binding of H+, K+, Na+ and Mg2+ to 21 metabolites of skeletal muscle non-mitochondrial energy metabolism, resulting in 104 different metabolite-cation complexes. Fractional binding of H+ to these metabolite-cation complexes were applied to 17 reactions of skeletal muscle non-mitochondrial energy metabolism, and 8 conditions of the glycolytic pathway based on the source of substrate (glycogen vs. glucose, completeness of glycolytic flux, and the end-point of pyruvate vs. lactate. For pH conditions of 6.0 and 7.0, respectively, H+ coefficients (-'ve values = H+ release for the creatine kinase, adenylate kinase, AMP deaminase and ATPase reactions were 0.8 and 0.97, -0.13 and -0.02, 1.2 and 1.09, and -0.01 and -0.66, respectively. The glycolytic pathway is net H+ releasing, regardless of lactate production, which consumes 1 H+. For glycolysis fueled by glycogen and ending in either pyruvate or lactate, H+ coefficients for pH 6.0 and 7.0 were -3.97 and -2.01 (pyruvate, and -1.96 and -0.01 (lactate, respectively. When starting with glucose, the same conditions result in H+ coefficients of -3.98 and -2.67, and -1.97 and -0.67, respectively. The most H+ releasing reaction of glycolysis is the glyceraldehyde-3-phosphate dehydrogenase reaction, with H+ coefficients for pH 6.0 and 7.0 of -1.58 and -0.76, respectively. Incomplete flux of substrate through glycolysis would increase net H+ release due to the absence of the pyruvate kinase and lactate dehydrogenase reactions, which collectively result in H+ coefficients for pH 6.0 and 7.0 of 1.35 and 1.88, respectively. The data presented provide an extensive reference source for academics and researchers to accurately profile the balance of protons for all metabolites and reactions of non

A comparison of new measurements of total monoterpene flux with improved measurements of speciated monoterpene flux

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A. Lee

2005-01-01

Full Text Available Many monoterpenes have been identified in forest emissions using gas chromatography (GC. Until now, it has been impossible to determine whether all monoterpenes are appropriately measured using GC techniques. We used a proton transfer reaction mass spectrometer (PTR-MS coupled with the eddy covariance (EC technique to measure mixing ratios and fluxes of total monoterpenes above a ponderosa pine plantation. We compared PTR-MS-EC results with simultaneous measurements of eight speciated monoterpenes, β-pinene, α-pinene, 3-carene, d-limonene, β-phellandrene, α-terpinene, camphene, and terpinolene, made with an automated, in situ gas chromatograph with flame ionization detectors (GC-FID, coupled to a relaxed eddy accumulation system (REA. Monoterpene mixing ratios and fluxes measured by PTR-MS averaged 30±2.3% and 31±9.2% larger than by GC-FID, with larger mixing ratio discrepancies between the two techniques at night than during the day. Two unidentified peaks that correlated with β-pinene were resolved in the chromatograms and completely accounted for the daytime difference and reduced the nighttime mixing ratio difference to 20±2.9%. Measurements of total monoterpenes by PTR-MS-EC indicated that GC-FID-REA measured the common, longer-lived monoterpenes well, but that additional terpenes were emitted from the ecosystem that represented an important contribution to the total mixing ratio above the forest at night.

Study of glycolytic intermediates in hereditary elliptocytosis with thalassemia

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Pavri Roshan

1977-01-01

Full Text Available Glycolytic intermediates like ATP, DPG and GSH have been studied in a family with. hereditary elliptocytosis and thalassemia. Results indicate a fall in ATP with a concomitant rise in DPG in the Patient. Findings are discussed in relation to other data.

Global CO2 fluxes estimated from GOSAT retrievals of total column CO2

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S. Basu

2013-09-01

Full Text Available We present one of the first estimates of the global distribution of CO2 surface fluxes using total column CO2 measurements retrieved by the SRON-KIT RemoTeC algorithm from the Greenhouse gases Observing SATellite (GOSAT. We derive optimized fluxes from June 2009 to December 2010. We estimate fluxes from surface CO2 measurements to use as baselines for comparing GOSAT data-derived fluxes. Assimilating only GOSAT data, we can reproduce the observed CO2 time series at surface and TCCON sites in the tropics and the northern extra-tropics. In contrast, in the southern extra-tropics GOSAT XCO2 leads to enhanced seasonal cycle amplitudes compared to independent measurements, and we identify it as the result of a land–sea bias in our GOSAT XCO2 retrievals. A bias correction in the form of a global offset between GOSAT land and sea pixels in a joint inversion of satellite and surface measurements of CO2 yields plausible global flux estimates which are more tightly constrained than in an inversion using surface CO2 data alone. We show that assimilating the bias-corrected GOSAT data on top of surface CO2 data (a reduces the estimated global land sink of CO2, and (b shifts the terrestrial net uptake of carbon from the tropics to the extra-tropics. It is concluded that while GOSAT total column CO2 provide useful constraints for source–sink inversions, small spatiotemporal biases – beyond what can be detected using current validation techniques – have serious consequences for optimized fluxes, even aggregated over continental scales.

Overexpression of Genes Encoding Glycolytic Enzymes in Corynebacterium glutamicum Enhances Glucose Metabolism and Alanine Production under Oxygen Deprivation Conditions

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Yamamoto, Shogo; Gunji, Wataru; Suzuki, Hiroaki; Toda, Hiroshi; Suda, Masako; Jojima, Toru; Inui, Masayuki

2012-01-01

We previously reported that Corynebacterium glutamicum strain ΔldhAΔppc+alaD+gapA, overexpressing glyceraldehyde-3-phosphate dehydrogenase-encoding gapA, shows significantly improved glucose consumption and alanine formation under oxygen deprivation conditions (T. Jojima, M. Fujii, E. Mori, M. Inui, and H. Yukawa, Appl. Microbiol. Biotechnol. 87:159–165, 2010). In this study, we employ stepwise overexpression and chromosomal integration of a total of four genes encoding glycolytic enzymes (herein referred to as glycolytic genes) to demonstrate further successive improvements in C. glutamicum glucose metabolism under oxygen deprivation. In addition to gapA, overexpressing pyruvate kinase-encoding pyk and phosphofructokinase-encoding pfk enabled strain GLY2/pCRD500 to realize respective 13% and 20% improved rates of glucose consumption and alanine formation compared to GLY1/pCRD500. Subsequent overexpression of glucose-6-phosphate isomerase-encoding gpi in strain GLY3/pCRD500 further improved its glucose metabolism. Notably, both alanine productivity and yield increased after each overexpression step. After 48 h of incubation, GLY3/pCRD500 produced 2,430 mM alanine at a yield of 91.8%. This was 6.4-fold higher productivity than that of the wild-type strain. Intracellular metabolite analysis showed that gapA overexpression led to a decreased concentration of metabolites upstream of glyceraldehyde-3-phosphate dehydrogenase, suggesting that the overexpression resolved a bottleneck in glycolysis. Changing ratios of the extracellular metabolites by overexpression of glycolytic genes resulted in reduction of the intracellular NADH/NAD+ ratio, which also plays an important role on the improvement of glucose consumption. Enhanced alanine dehydrogenase activity using a high-copy-number plasmid further accelerated the overall alanine productivity. Increase in glycolytic enzyme activities is a promising approach to make drastic progress in growth-arrested bioprocesses. PMID

Preslaughter handling effects on pork quality and glycolytic potential in two muscles differing in fiber type composition

NARCIS (Netherlands)

Hambrecht, E.; Eissen, J.J.; Newman, D.J.; Smits, C.H.M.; Verstegen, M.W.A.; Hartog, den L.A.

2005-01-01

The objective of the present experiment was to investigate the effects of transportation, lairage, and preslaughter stressor treatment on glycolytic potential and pork quality of the glycolytic longissimus and the oxidative supraspinatus (SSP) or serratus ventralis (SV) muscles. In a 2 x 2 x 2

Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production.

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Carinhas, Nuno; Pais, Daniel A M; Koshkin, Alexey; Fernandes, Paulo; Coroadinha, Ana S; Carrondo, Manuel J T; Alves, Paula M; Teixeira, Ana P

2016-03-23

Canine adenovirus vector type 2 (CAV2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. However, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. Combining [1,2-(13)C]glucose and [U-(13)C]glutamine, we apply for the first time (13)C-Metabolic flux analysis ((13)C-MFA) to study E1-transformed Madin-Darby Canine Kidney (MDCK) cells metabolism during growth and CAV2 production. MDCK cells displayed a marked glycolytic and ammoniagenic metabolism, and (13)C data revealed a large fraction of glutamine-derived labelling in TCA cycle intermediates, emphasizing the role of glutamine anaplerosis. (13)C-MFA demonstrated the importance of pyruvate cycling in balancing glycolytic and TCA cycle activities, as well as occurrence of reductive alphaketoglutarate (AKG) carboxylation. By turn, CAV2 infection significantly upregulated fluxes through most central metabolism, including glycolysis, pentose-phosphate pathway, glutamine anaplerosis and, more prominently, reductive AKG carboxylation and cytosolic acetyl-coenzyme A formation, suggestive of increased lipogenesis. Based on these results, we suggest culture supplementation strategies to stimulate nucleic acid and lipid biosynthesis for improved canine adenoviral vector production.

Similar temperature dependencies of glycolytic enzymes: an evolutionary adaptation to temperature dynamics?

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Cruz Luisa Ana B

2012-12-01

Full Text Available Abstract Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes. Results Saccharomyces cerevisiae was grown under different temperature regimes and glucose availability conditions. These included glucose-excess batch cultures at different temperatures and glucose-limited chemostat cultures, subjected to fast linear temperature shifts and circadian sinoidal temperature cycles. An observed temperature-independent relation between intracellular levels of glycolytic metabolites and residual glucose concentration for all experimental conditions revealed that it is the substrate availability rather than temperature that determines intracellular metabolite profiles. This observation corresponded with predictions generated in silico with a kinetic model of yeast glycolysis, when the catalytic capacities of all glycolytic enzymes were set to share the same normalized temperature dependency. Conclusions From an evolutionary perspective, such similar temperature dependencies allow cells to adapt more rapidly to temperature changes, because they result in minimal perturbations of intracellular metabolite levels, thus circumventing the need for extensive modification of enzyme levels.

The unique features of glycolytic pathways in Archaea.

OpenAIRE

Verhees, Corné H; Kengen, Servé W M; Tuininga, Judith E; Schut, Gerrit J; Adams, Michael W W; De Vos, Willem M; Van Der Oost, John

2003-01-01

An early divergence in evolution has resulted in two prokaryotic domains, the Bacteria and the Archaea. Whereas the central metabolic routes of bacteria and eukaryotes are generally well-conserved, variant pathways have developed in Archaea involving several novel enzymes with a distinct control. A spectacular example of convergent evolution concerns the glucose-degrading pathways of saccharolytic archaea. The identification, characterization and comparison of the glycolytic enzymes of a vari...

Concentration, flux, and the analysis of trends of total and dissolved phosphorus, total nitrogen, and chloride in 18 tributaries to Lake Champlain, Vermont and New York, 1990–2011

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Medalie, Laura

2013-01-01

Annual concentration, flux, and yield for total phosphorus, dissolved phosphorus, total nitrogen, and chloride for 18 tributaries to Lake Champlain were estimated for 1990 through 2011 using a weighted regression method based on time, tributary streamflows (discharges), and seasonal factors. The weighted regression method generated two series of daily estimates of flux and concentration during the period of record: one based on observed discharges and a second based on a flow-normalization procedure that removes random variation due to year-to-year climate-driven effects. The flownormalized estimate for a given date is similar to an average estimate of concentration or flux that would be made if all of the observed discharges for that date were equally likely to have occurred. The flux bias statistic showed that 68 of the 72 flux regression models were minimally biased. Temporal trends in the concentrations and fluxes were determined by calculating percent changes in flow-normalized annual fluxes for the full period of analysis (1990 through 2010) and for the decades 1990–2000 and 2000–2010. Basinwide, flow-normalized total phosphorus flux decreased by 42 metric tons per year (t/yr) between 1990 and 2010. This net result reflects a basinwide decrease in flux of 21 metric tons (t) between 1990 and 2000, followed by a decrease of 20 t between 2000 and 2010; both results were largely influenced by flux patterns in the large tributaries on the eastern side of the basin. A comparison of results for total phosphorus for the two separate decades of analysis found that more tributaries had decreasing concentrations and flux rates in the second decade than the first. An overall reduction in dissolved phosphorus flux of 0.7 t/yr was seen in the Lake Champlain Basin during the full period of analysis. That very small net change in flux reflects substantial reductions between 1990 and 2000 from eastern tributaries, especially in Otter Creek and the LaPlatte and Winooski

Streamwater fluxes of total mercury and methylmercury into and out of Lake Champlain

International Nuclear Information System (INIS)

Shanley, James B.; Chalmers, Ann T.

2012-01-01

From 2000 to 2004, we sampled for total mercury (THg) and methylmercury (MeHg) in inlet streams to Lake Champlain, targeting high flow periods to capture increases in THg and MeHg concentrations with increasing flow. We used these data to model stream THg and MeHg fluxes for Water Years 2001 through 2009. In this mountainous forested basin with a high watershed-to-lake area ratio of 18, fluvial export from the terrestrial watershed was the dominant source of Hg to the lake. Unfiltered THg and MeHg fluxes were dominated by the particulate fraction; about 40% of stream THg was in the filtered ( −2 yr −1 , or about 13% of atmospheric Hg wet and dry deposition to the basin. THg export from the lake represented only about 3% of atmospheric Hg input to the basin. - Highlights: ► We monitored total mercury and methylmercury in major tributaries to Lake Champlain. ► Mercury and methylmercury export was primarily as particulates during high flow. ► Only 13% of atmospheric total mercury input reached the lake via streams. ► Only 3% of atmospheric total mercury input reached the lake outlet. - Eighty-seven percent of total mercury deposition to the Lake Champlain basin is retained in the terrestrial basin; stream export of total and methylmercury to the lake is primarily in the particulate phase.

Impact of Autoantibodies against Glycolytic Enzymes on Pathogenicity of Autoimmune Retinopathy and Other Autoimmune Disorders

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Grazyna Adamus

2017-04-01

Full Text Available Autoantibodies (AAbs against glycolytic enzymes: aldolase, α-enolase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase are prevalent in sera of patients with blinding retinal diseases, such as paraneoplastic [cancer-associated retinopathy (CAR] and non-paraneoplastic autoimmune retinopathies, as well as in many other autoimmune diseases. CAR is a degenerative disease of the retina characterized by sudden vision loss in patients with cancer and serum anti-retinal AAbs. In this review, we discuss the widespread serum presence of anti-glycolytic enzyme AAbs and their significance in autoimmune diseases. There are multiple mechanisms responsible for antibody generation, including the innate anti-microbial response, anti-tumor response, or autoimmune response against released self-antigens from damaged, inflamed tissue. AAbs against enolase, GADPH, and aldolase exist in a single patient in elevated titers, suggesting their participation in pathogenicity. The lack of restriction of AAbs to one disease may be related to an increased expression of glycolytic enzymes in various metabolically active tissues that triggers an autoimmune response and generation of AAbs with the same specificity in several chronic and autoimmune conditions. In CAR, the importance of serum anti-glycolytic enzyme AAbs had been previously dismissed, but the retina may be without pathological consequence until a failure of the blood–retinal barrier function, which would then allow pathogenic AAbs access to their retinal targets, ultimately leading to damaging effects.

Effects of Glycolytic-Based Interval Training on Anaerobic Capacity in Soccer Players

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Polczyk Michał

2015-09-01

Full Text Available Purpose. The aim of this study was to determine the magnitude of changes in anaerobic endurance in response to a training protocol targeting glycolytic capacity. Methods. The study involved 24 soccer players from two U-18 teams. One team served as an experimental (E group the other a control (C. Besides standard soccer practice performed by both groups, an interval training protocol was administered to the experimental group twice a week (15 sessions. One training repetition involved running a soccerspecific course. Repetition time was equal to 15 s interspersed with 45 s passive recovery. Total number of repetitions was determined by the ability to maintain target time (power in subsequent repetitions. A 5% reduction in the distance covered (m compared with the first repetition ended a set. The number of sets was based on the ability of player to maintain target time per repetition. Rest interval between sets was 15 min. Anaerobic performance was assessed before and after the 8-week protocol by the Wingate test in which arterial blood gases, blood lactate concentration, and respiratory variables on a breath-by-breath basis were measured. Results. Distance covered in group E in the first training session was 470.38 ± 77.82 m and 1182.31 ± 164.44 m in the last session. Post-intervention total work (273.63 ± 18.32 to 284.98 ± 15.76 J/kg and maximum power (13.28 ± 1.43 to 14.14 ± 1.25 W/kg significantly increased in the Wingate test. Statistically significant increases in lactate concentration (10.64 ± 1.54 and 12.72 ± 1.59 mmol/l and lower blood pH (7.21 ± 0.03 and 7.19 ± 0.02 were also observed. No significant changes in any of the above variables were observed in group C. Conclusions. Interval training develops glycolytic capacity but with large inter-individual variability.

Similar temperature dependencies of glycolytic enzymes : An evolutionary adaptation to temperature dynamics?

NARCIS (Netherlands)

Cruz, L.A.B.; Hebly, M.; Duong, G.H.; Wahl, S.A.; Pronk, J.T.; Heijnen, J.J.; Daran-Lapujade, P.; Van Gulik, W.M.

2012-01-01

Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in

Switch between life history strategies due to changes in glycolytic enzyme gene dosage in Saccharomyces cerevisiae.

Science.gov (United States)

Wang, Shaoxiao; Spor, Aymé; Nidelet, Thibault; Montalent, Pierre; Dillmann, Christine; de Vienne, Dominique; Sicard, Delphine

2011-01-01

Adaptation is the process whereby a population or species becomes better fitted to its habitat through modifications of various life history traits which can be positively or negatively correlated. The molecular factors underlying these covariations remain to be elucidated. Using Saccharomyces cerevisiae as a model system, we have investigated the effects on life history traits of varying the dosage of genes involved in the transformation of resources into energy. Changing gene dosage for each of three glycolytic enzyme genes (hexokinase 2, phosphoglucose isomerase, and fructose-1,6-bisphosphate aldolase) resulted in variation in enzyme activities, glucose consumption rate, and life history traits (growth rate, carrying capacity, and cell size). However, the range of effects depended on which enzyme was expressed differently. Most interestingly, these changes revealed a genetic trade-off between carrying capacity and cell size, supporting the discovery of two extreme life history strategies already described in yeast populations: the "ants," which have lower glycolytic gene dosage, take up glucose slowly, and have a small cell size but reach a high carrying capacity, and the "grasshoppers," which have higher glycolytic gene dosage, consume glucose more rapidly, and allocate it to a larger cell size but reach a lower carrying capacity. These results demonstrate antagonist pleiotropy for glycolytic genes and show that altered dosage of a single gene drives a switch between two life history strategies in yeast.

Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells.

Science.gov (United States)

Chen, Jing; Hu, Xun; Cui, Jingjie

2018-05-01

Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin K 3 and vitamin K 5 , have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity. In the present study, enzymatic assays were performed using MCF-7 cell lysate in order to screen the profile of glycolytic enzymes in cancer cells inhibited by shikonin, vitamin K 3 and vitamin K 5 , in addition to pyruvate kinase. Results revealed that hexokinase, phosphofructokinase-1, fructose bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase produced in the process of glycolysis were inhibited by shikonin, vitamin K 3 and vitamin K 5 . The results indicated that shikonin, vitamin K 3 and vitamin K 5 are chemical inhibitors of glycolytic enzymes in cancer cells and have potential uses in translational medical applications.

Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria.

Science.gov (United States)

Sánchez-Pascuala, Alberto; Nikel, Pablo I; de Lorenzo, Víctor

2018-01-01

The Embden-Meyerhof-Parnas (EMP) pathway is widely accepted to be the biochemical standard of glucose catabolism. The well-characterized glycolytic route of Escherichia coli, based on the EMP catabolism, is an example of an intricate pathway in terms of genomic organization of the genes involved and patterns of gene expression and regulation. This intrinsic genetic and metabolic complexity renders it difficult to engineer glycolytic activities and transfer them onto other microbial cell factories, thus limiting the biotechnological potential of bacterial hosts that lack the route. Taking into account the potential applications of such a portable tool for targeted pathway engineering, in the present protocol we describe how the genes encoding all the enzymes of the linear EMP route have been individually recruited from the genome of E. coli K-12, edited in silico to remove their endogenous regulatory signals, and synthesized de novo following a standard (i.e., GlucoBrick) that facilitates their grouping in the form of functional modules that can be combined at the user's will. This novel genetic tool allows for the à la carte implementation or boosting of EMP pathway activities into different Gram-negative bacteria. The potential of the GlucoBrick platform is further illustrated by engineering novel glycolytic activities in the most representative members of the Pseudomonas genus (Pseudomonas putida and Pseudomonas aeruginosa).

Quantitative proteomics of the tonoplast reveals a role for glycolytic enzymes in salt tolerance.

Science.gov (United States)

Barkla, Bronwyn J; Vera-Estrella, Rosario; Hernández-Coronado, Marcela; Pantoja, Omar

2009-12-01

To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na(+) sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H(+)-ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H(+)-pump activity.

Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection

Energy Technology Data Exchange (ETDEWEB)

Kohio, Hinissan P.; Adamson, Amy L., E-mail: aladamso@uncg.edu

2013-09-15

As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. - Highlights: • Increased glucose levels increase Influenza A viral infection of MDCK cells. • Inhibition of the glycolytic enzyme hexokinase inhibited Influenza A viral infection. • Inhibition of hexokinase induced disassembly the V-ATPase. • Disassembly of the V-ATPase and Influenza A infection was bypassed with ATP. • The state of V-ATPase assembly correlated with Influenza A infection of cells.

Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection

International Nuclear Information System (INIS)

Kohio, Hinissan P.; Adamson, Amy L.

2013-01-01

As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. - Highlights: • Increased glucose levels increase Influenza A viral infection of MDCK cells. • Inhibition of the glycolytic enzyme hexokinase inhibited Influenza A viral infection. • Inhibition of hexokinase induced disassembly the V-ATPase. • Disassembly of the V-ATPase and Influenza A infection was bypassed with ATP. • The state of V-ATPase assembly correlated with Influenza A infection of cells

Metabolomic Analysis of Oxidative and Glycolytic Skeletal Muscles by Matrix-Assisted Laser Desorption/IonizationMass Spectrometric Imaging (MALDI MSI)

Science.gov (United States)

Tsai, Yu-Hsuan; Garrett, Timothy J.; Carter, Christy S.; Yost, Richard A.

2015-06-01

Skeletal muscles are composed of heterogeneous muscle fibers that have different physiological, morphological, biochemical, and histological characteristics. In this work, skeletal muscles extensor digitorum longus, soleus, and whole gastrocnemius were analyzed by matrix-assisted laser desorption/ionization mass spectrometry to characterize small molecule metabolites of oxidative and glycolytic muscle fiber types as well as to visualize biomarker localization. Multivariate data analysis such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to extract significant features. Different metabolic fingerprints were observed from oxidative and glycolytic fibers. Higher abundances of biomolecules such as antioxidant anserine as well as acylcarnitines were observed in the glycolytic fibers, whereas taurine and some nucleotides were found to be localized in the oxidative fibers.

Modeling diauxic glycolytic oscillations in yeast

DEFF Research Database (Denmark)

Hald, Bjørn Olav; Sørensen, Preben Graae

2010-01-01

for investigations of central metabolism dynamics of yeast cells. We have previously proposed a model for the open system comprised of the primary fermentative reactions in yeast that quantitatively describes the oscillatory dynamics. However, this model fails to describe the transient behavior of metabolic......Glycolytic oscillations in a stirred suspension of starved yeast cells is an excellent model system for studying the dynamics of metabolic switching in living systems. In an open-flow system the oscillations can be maintained indefinitely at a constant operating point where they can....... Experimental and computational results strongly suggest that regulation of acetaldehyde explains the observed behavior. We have extended the original model with regulation of pyruvate decarboxylase, a reversible alcohol dehydrogenase, and drainage of pyruvate. Using the method of time rescaling in the extended...

Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria

DEFF Research Database (Denmark)

Sánchez-Pascuala, Alberto; Nikel, Pablo I.; de Lorenzo, Víctor

2018-01-01

the potential applications of such a portable tool for targeted pathway engineering, in the present protocol we describe how the genes encoding all the enzymes of the linear EMP route have been individually recruited from the genome of E. coli K-12, edited in silico to remove their endogenous regulatory signals......The Embden-Meyerhof-Parnas (EMP) pathway is widely accepted to be the biochemical standard of glucose catabolism. The well-characterized glycolytic route of Escherichia coli, based on the EMP catabolism, is an example of an intricate pathway in terms of genomic organization of the genes involved...... and patterns of gene expression and regulation. This intrinsic genetic and metabolic complexity renders it difficult to engineer glycolytic activities and transfer them onto other microbial cell factories, thus limiting the biotechnological potential of bacterial hosts that lack the route. Taking into account...

Concentration, flux, and trend estimates with uncertainty for nutrients, chloride, and total suspended solids in tributaries of Lake Champlain, 1990–2014

Science.gov (United States)

Medalie, Laura

2016-12-20

The U.S. Geological Survey, in cooperation with the New England Interstate Water Pollution Control Commission and the Vermont Department of Environmental Conservation, estimated daily and 9-month concentrations and fluxes of total and dissolved phosphorus, total nitrogen, chloride, and total suspended solids from 1990 (or first available date) through 2014 for 18 tributaries of Lake Champlain. Estimates of concentration and flux, provided separately in Medalie (2016), were made by using the Weighted Regressions on Time, Discharge, and Season (WRTDS) regression model and update previously published WRTDS model results with recent data. Assessment of progress towards meeting phosphorus-reduction goals outlined in the Lake Champlain management plan relies on annual estimates of phosphorus flux. The percent change in annual concentration and flux is provided for two time periods. The R package EGRETci was used to estimate the uncertainty of the trend estimate. Differences in model specification and function between this study and previous studies that used WRTDS to estimate concentration and flux using data from Lake Champlain tributaries are described. Winter data were too sparse and nonrepresentative to use for estimates of concentration and flux but were sufficient for estimating the percentage of total annual flux over the period of record. Median winter-to-annual fractions ranged between 21 percent for total suspended solids and 27 percent for dissolved phosphorus. The winter contribution was largest for all constituents from the Mettawee River and smallest from the Ausable River. For the full record (1991 through 2014 for total and dissolved phosphorus and chloride and 1993 through 2014 for nitrogen and total suspended solids), 6 tributaries had decreasing trends in concentrations of total phosphorus, and 12 had increasing trends; concentrations of dissolved phosphorus decreased in 6 and increased in 8 tributaries; fluxes of total phosphorus decreased in 5 and

Frequent and recent retrotransposition of orthologous genes plays a role in the evolution of sperm glycolytic enzymes

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de Villena Fernando

2010-05-01

Full Text Available Abstract Background The central metabolic pathway of glycolysis converts glucose to pyruvate, with the net production of 2 ATP and 2 NADH per glucose molecule. Each of the ten reactions in this pathway is typically catalyzed by multiple isozymes encoded by a multigene family. Several isozymes in this pathway are expressed only during spermatogenesis, and gene targeting studies indicate that they are essential for sperm function and male fertility in mouse. At least three of the novel glycolytic isozymes are encoded by retrogenes (Pgk2, Aldoart1, and Aldoart2. Their restricted expression profile suggests that retrotransposition may play a significant role in the evolution of sperm glycolytic enzymes. Results We conducted a comprehensive genomic analysis of glycolytic enzymes in the human and mouse genomes and identified several intronless copies for all enzymes in the pathway, except Pfk. Within each gene family, a single orthologous gene was typically retrotransposed frequently and independently in both species. Several retroposed sequences maintained open reading frames (ORFs and/or provided evidence of alternatively spliced exons. We analyzed expression of sequences with ORFs and Gpi1 transcript in mouse spermatogenic cells. Conclusions Our analysis detected frequent, recent, and lineage-specific retrotransposition of orthologous glycolytic enzymes in the human and mouse genomes. Retrotransposition events are associated with LINE/LTR and genomic integration is random. We found evidence for the alternative splicing of parent genes. Many retroposed sequences have maintained ORFs, suggesting a functional role for these genes.

Total and methyl mercury concentrations and fluxes from small boreal forest catchments in Finland

International Nuclear Information System (INIS)

Porvari, Petri; Verta, Matti

2003-01-01

Peatlands have higher methyl mercury output than uplands. - Total mercury (TotHg) and methyl mercury (MeHg) concentrations were studied in runoff from eight small (0.02-1.3 km 2 ) boreal forest catchments (mineral soil and peatland) during 1990-1995. Runoff waters were extremely humic (TOC 7-70 mg l -1 ). TotHg concentrations varied between 0.84 and 24 ng l -1 and MeHg between 0.03 and 3.8 ng l -1 . TotHg fluxes from catchments ranged from 0.92 to 1.8 g km -2 a -1 , and MeHg fluxes from 0.03 to 0.33 g km -2 a -1 . TotHg concentrations and output fluxes measured in runoff water from small forest catchments in Finland were comparable with those measured in other boreal regions. By contrast, MeHg concentrations were generally higher. Estimates for MeHg output fluxes in this study were comparable at sites with forests and wetlands in Sweden and North America, but clearly higher than those measured at upland or agricultural sites in other studies. Peatland catchments released more MeHg than pure mineral soil or mineral soil catchments with minor area of peatland

The Structural and Functional Coordination of Glycolytic Enzymes in Muscle: Evidence of a Metabolon?

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Lynda Menard

2014-09-01

Full Text Available Metabolism sustains life through enzyme-catalyzed chemical reactions within the cells of all organisms. The coupling of catalytic function to the structural organization of enzymes contributes to the kinetic optimization important to tissue-specific and whole-body function. This coupling is of paramount importance in the role that muscle plays in the success of Animalia. The structure and function of glycolytic enzyme complexes in anaerobic metabolism have long been regarded as a major regulatory element necessary for muscle activity and whole-body homeostasis. While the details of this complex remain to be elucidated through in vivo studies, this review will touch on recent studies that suggest the existence of such a complex and its structure. A potential model for glycolytic complexes and related subcomplexes is introduced.

Methods of total spectral radiant flux realization at VNIIOFI

Science.gov (United States)

Ivashin, Evgeniy; Lalek, Jan; Rybczyński, Andrzej; Ogarev, Sergey; Khlevnoy, Boris; Dobroserdov, Dmitry; Sapritsky, Victor

2018-02-01

VNIIOFI carries out works on realization of independent methods for realization of the total spectral radiant flux (TSRF) of incoherent optical radiation sources - reference high-temperature blackbodies (BB), halogen lamps, and LED with quasi-Lambert spatial distribution of radiance. The paper describes three schemes for measuring facilities using photometers, spectroradiometers and computer-controlled high class goniometer. The paper describes different approaches for TSRF realization at the VNIIOFI National radiometric standard on the basis of high-temperature BB and LED sources, and gonio-spectroradiometer. Further, they are planned to be compared, and the use of fixed-point cells (in particular, based on the high-temperature δ(MoC)-C metal-carbon eutectic with a phase transition temperature of 2583 °C corresponding to the metrological optical “source-A”) as an option instead of the BB is considered in order to enhance calibration accuracy.

Blocking CD147 induces cell death in cancer cells through impairment of glycolytic energy metabolism

International Nuclear Information System (INIS)

Baba, Miyako; Inoue, Masahiro; Itoh, Kazuyuki; Nishizawa, Yasuko

2008-01-01

CD147 is a multifunctional transmembrane protein and promotes cancer progression. We found that the anti-human CD147 mouse monoclonal antibody MEM-M6/1 strongly induces necrosis-like cell death in LoVo, HT-29, WiDr, and SW620 colon cancer cells and A2058 melanoma cells, but not in WI-38 and TIG-113 normal fibroblasts. Silencing or overexpression of CD147 in LoVo cells enhanced or decreased the MEM-M6/1 induced cell death, respectively. CD147 is known to form complex with proton-linked monocarboxylate transporters (MCTs), which is critical for lactate transport and intracellular pH (pHi) homeostasis. In LoVo cells, CD147 and MCT-1 co-localized on the cell surface, and MEM-M6/1 inhibited the association of these molecules. MEM-M6/1 inhibited lactate uptake, lactate release, and reduced pHi. Further, the induction of acidification was parallel to the decrease of the glycolytic flux and intracellular ATP levels. These effects were not found in the normal fibroblasts. As cancer cells depend on glycolysis for their energy production, CD147 inhibition might induce cell death specific to cancer cells

Scaling of oxidative and glycolytic enzymes in mammals.

Science.gov (United States)

Emmett, B; Hochachka, P W

1981-09-01

The catalytic activities of several oxidative and glycolytic enzymes were determined in the gastrocnemius muscle of 10 mammalian species differing in body weight by nearly 6 orders of magnitude. When expressed in terms of units gm-1, the activities of enzymes functioning in oxidative metabolism (citrate synthase, beta-hydroxybutyrylCoA dehydrogenase, and malate dehydrogenase) decrease as body weight increases. Log-log plots (activity gm-1 vs body mass) yield straight lines with negative slopes that are less than the allometric exponent (-0.25) typically observed for basal metabolic rates. Since the amount of power a muscle can generate depends upon the catalytic potential of its enzyme machinery (the higher the catalytic potential the higher the maximum rate of energy generation), these data predict that the scope for aerobic activity in large mammals should be greater than in small mammals if nothing else becomes limiting, a result in fact recently obtained by Taylor et al. (Respir. Physiol., 1981). In contrast to the scaling of oxidative enzymes, the activities of enzymes functioning in anaerobic glycogenolysis (glycogen phosphorylase, pyruvate kinase, and lactate dehydrogenase) increase as body size increases. Log-log plots (activity gm-1 vs body mass) display a positive slope indicating that the larger the animal the higher the glycolytic potential of its skeletal muscles. This unexpected result may indicate higher relative power costs for burst type locomotion in larger mammals, which is in fact observed in within-species studies of man. However, the scaling of anaerobic muscle power has not been closely assessed in between-species comparisons of mammals varying greatly in body size.

Glycolytic inhibitors 2-deoxyglucose and 3-bromopyruvate synergize with photodynamic therapy respectively to inhibit cell migration.

Science.gov (United States)

Feng, Xiaolan; Wang, Pan; Liu, Quanhong; Zhang, Ting; Mai, Bingjie; Wang, Xiaobing

2015-06-01

Most cancer cells have the specially increased glycolytic phenotype, which makes this pathway become an attractive therapeutic target. Although glycolytic inhibitor 2-deoxyglucose (2-DG) has been demonstrated to potentiate the cytotoxicity of photodynamic therapy (PDT), the impacts on cell migration after the combined treatment has never been reported yet. The present study aimed to analyze the influence of glycolytic inhibitors 2-DG and 3-bromopyruvate (3-BP) combined with Ce6-PDT on cell motility of Triple Negative Breast Cancer MDA-MB-231 cells. As determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium-bromide-Tetraz-olium (MTT) assay, more decreased cell viability was observed in 2-DG + PDT and 3-BP + PDT groups when compared with either monotherapy. Under optimal conditions, synergistic potentiation on cell membrane destruction and the decline of cell adhesion and cells migratory ability were observed in both 2-DG + PDT and 3-BP + PDT by electron microscope observation (SEM), wound healing and trans-well assays. Besides, serious microfilament network collapses as well as impairment of matrix metalloproteinases-9 (MMP-9) were notably improved after the combined treatments by immunofluorescent staining. These results suggest that 2-DG and 3-BP can both significantly potentiated Ce6-PDT efficacy of cell migration inhibition.

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance[C][W

Science.gov (United States)

Barkla, Bronwyn J.; Vera-Estrella, Rosario; Hernández-Coronado, Marcela; Pantoja, Omar

2009-01-01

To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na+ sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H+-ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H+-pump activity. PMID:20028841

Observations of atmosphere-biosphere exchange of total and speciated peroxynitrates: nitrogen fluxes and biogenic sources of peroxynitrates

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K.-E. Min

2012-10-01

Full Text Available Peroxynitrates are responsible for global scale transport of reactive nitrogen. Recent laboratory observations suggest that they may also play an important role in delivery of nutrients to plant canopies. We measured eddy covariance fluxes of total peroxynitrates (ΣPNs and three individual peroxynitrates (APNs ≡ PAN + PPN + MPAN over a ponderosa pine forest during the Biosphere Effects on AeRosols and Photochemistry EXperiment 2009 (BEARPEX 2009. Concentrations of these species were also measured at multiple heights above and within the canopy. While the above-canopy daytime concentrations are nearly identical for ΣPNs and APNs, we observed the downward flux of ΣPNs to be 30–60% slower than the flux of APNs. The vertical concentration gradients of ΣPNs and APNs vary with time of day and exhibit different temperature dependencies. These differences can be explained by the production of peroxynitrates other than PAN, PPN, and MPAN within the canopy (presumably as a consequence of biogenic VOC emissions and upward fluxes of these PN species. The impact of this implied peroxynitrate flux on the interpretation of NO_x fluxes and ecosystem N exchange is discussed.

Measurement of the nue and Total 8B Solar Neutrino Fluxes with theSudbury Neutrino Observatory Phase I Data Set

Energy Technology Data Exchange (ETDEWEB)

Aharmim, B.; Ahmad, Q.R.; Ahmed, S.N.; Allen, R.C.; Andersen,T.C.; Anglin, J.D.; Buehler, G.; Barton, J.C.; Beier, E.W.; Bercovitch,M.; Bergevin, M.; Bigu, J.; Biller, S.D.; Black, R.A.; Blevis, I.; Boardman, R.J.; Boger, J.; Bonvin, E.; Boulay, M.G.; Bowler, M.G.; Bowles, T.J.; Brice, S.J.; Browne, M.C.; Bullard, T.V.; Burritt, T.H.; Cameron, J.; Chan, Y.D.; Chen, H.H.; Chen, M.; Chen, X.; Cleveland, B.T.; Cowan, J.H.M.; Cowen, D.F.; Cox, G.A.; Currat, C.A.; Dai, X.; Dalnoki-Veress, F.; Davidson, W.F.; Deng, H.; DiMarco, M.; Doe, P.J.; Doucas, G.; Dragowsky, M.R.; Duba, C.A.; Duncan, F.A.; Dunford, M.; Dunmore, J.A.; Earle, E.D.; Elliott, S.R.; Evans, H.C.; Ewan, G.T.; Farine, J.; Fergani, H.; Ferraris, A.P.; Fleurot, F.; Ford, R.J.; Formaggio, J.A.; Fowler, M.M.; Frame, K.; Frank, E.D.; Frati, W.; Gagnon,N.; Germani, J.V.; Gil, S.; Goldschmidt, A.; Goon, J.T.M.; Graham, K.; Grant, D.R.; Guillian, E.; Hahn, R.L.; Hallin, A.L.; Hallman, E.D.; Hamer, A.S.; Hamian, A.A.; Handler, W.B.; Haq, R.U.; Hargrove, C.K.; Harvey, P.J.; Hazama, R.; Heeger, K.M.; Heintzelman, W.J.; Heise, J.; Helmer, R.L.; Henning, R.; Hepburn, J.D.; Heron, H.; Hewett, J.; Hime,A.; Howard, C.; Howe, M.A.; Huang, M.; Hykawy, J.G.; Isaac, M.C.P.; Jagam, P.; Jamieson, B.; Jelley, N.A.; Jillings, C.; Jonkmans, G.; Kazkaz, K.; Keener, P.T.; Kirch, K.; Klein, J.R.; Knox, A.B.; Komar,R.J.; Kormos, L.L.; Kos, M.; Kouzes, R.; Krueger, A.; Kraus, C.; Krauss,C.B.; Kutter, T.; Kyba, C.C.M.; Labranche, H.; Lange, R.; Law, J.; Lawson, I.T.; Lay, M.; Lee, H.W.; Lesko, K.T.; Leslie, J.R.; Levine, I.; Loach, J.C.; Locke, W.; Luoma, S.; Lyon, J.; MacLellan, R.; Majerus, S.; Mak, H.B.; Maneira, J.; Marino, A.D.; Martin, R.; McCauley, N.; McDonald,A.B.; McDonald, D.S.; McFarlane, K.; McGee, S.; McGregor, G.; MeijerDrees, R.; Mes, H.; Mifflin, C.; Miknaitis, K.K.S.; Miller, M.L.; Milton,G.; Moffat, B.A.; Monreal, B.; Moorhead, M.; Morrissette, B.; Nally,C.W.; Neubauer, M.S.; et al.

2007-02-01

This article provides the complete description of resultsfrom the Phase I data set of the Sudbury Neutrino Observatory (SNO). ThePhase I data set is based on a 0.65 kt-year exposure of heavy water tothe solar 8B neutrino flux. Included here are details of the SNO physicsand detector model, evaluations of systematic uncertainties, andestimates of backgrounds. Also discussed are SNO's approach tostatistical extraction of the signals from the three neutrino reactions(charged current, neutral current, and elastic scattering) and theresults of a search for a day-night asymmetry in the ?e flux. Under theassumption that the 8B spectrum is undistorted, the measurements fromthis phase yield a solar ?e flux of ?(?e) =1.76+0.05?0.05(stat.)+0.09?0.09 (syst.) x 106 cm?2 s?1, and a non-?ecomponent ?(? mu) = 3.41+0.45?0.45(stat.)+0.48?0.45 (syst.) x 106 cm?2s?1. The sum of these components provides a total flux in excellentagreement with the predictions of Standard Solar Models. The day-nightasymmetry in the ?e flux is found to be Ae = 7.0 +- 4.9 (stat.)+1.3?1.2percent (sys.), when the asymmetry in the total flux is constrained to bezero.

Capillary network in slow and fast muscles and in oxidative and glycolytic muscle fibres

Czech Academy of Sciences Publication Activity Database

Čebašek, V.; Kubínová, Lucie; Ribarič, S.; Eržen, I.

2005-01-01

Roč. 24, March (2005), s. 51-58 ISSN 1580-3139 Grant - others:SI-CZ(CZ) KONTAKT 19/2005 Institutional research plan: CEZ:AV0Z50110509 Keywords : capillaries * skeletal muscle fibre s-oxidative and glycolytic * stereology Subject RIV: EA - Cell Biology

Exploiting Unique Structural and Functional Properties of Malarial Glycolytic Enzymes for Antimalarial Drug Development

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Asrar Alam

2014-01-01

Full Text Available Metabolic enzymes have been known to carry out a variety of functions besides their normal housekeeping roles known as “moonlighting functions.” These functionalities arise from structural changes induced by posttranslational modifications and/or binding of interacting proteins. Glycolysis is the sole source of energy generation for malaria parasite Plasmodium falciparum, hence a potential pathway for therapeutic intervention. Crystal structures of several P. falciparum glycolytic enzymes have been solved, revealing that they exhibit unique structural differences from the respective host enzymes, which could be exploited for their selective targeting. In addition, these enzymes carry out many parasite-specific functions, which could be of potential interest to control parasite development and transmission. This review focuses on the moonlighting functions of P. falciparum glycolytic enzymes and unique structural differences and functional features of the parasite enzymes, which could be exploited for therapeutic and transmission blocking interventions against malaria.

GLYCOLYTIC AND ENERGETIC RESOURCES AS THE DETERMINANTS OF MEAT QUALITY OF DUROC FATTENERS

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Andrzej Zybert

2015-03-01

Full Text Available The aim of this study was to determine the diagnostic value of glycolityc and energetical quantities on selected meat quality characteristics of Duroc fatteners. A total of 40 Duroc porkers were investigated. Among two analysed sets of determinants (R1 with glycogen and R1 with lactate measured in 45 min after slaughter, the best diagnostic value for meat quality characteristics exhibit a set involving R1 and glycogen that composed determination coefficient (RC2 was 0.66 for pH measured in 45 min up to 144 h post mortem. Also, with currently used meat diagnostic methods the most useful one that explains the glycolytic and energetic quantities in the highest degree is method that exploits 5 determinants, i.e. pH1, pH24, EC2, EC24 and L*24. Higher composed determination coefficient and canonical correlation (CR for this method was obtained for set involving R1 and lactate – 0.41 and 0.64** respectively.

Enzyme mechanisms for pyruvate-to-lactate flux attenuation: a study of Sherpas, Quechuas, and hummingbirds.

Science.gov (United States)

Hochachka, P W; Stanley, C; McKenzie, D C; Villena, A; Monge, C

1992-10-01

During incremental exercise to fatigue under hypobaric hypoxia, Andean Quechua natives form and accumulate less plasma lactate than do lowlanders under similar conditions. This phenomenon of low lactate accumulation despite hypobaric hypoxia, first discovered some half century ago, is known in Quechuas to be largely unaffected by acute exposure to hypoxia or by acclimatization to sea level conditions. Earlier Nuclear Magnetic Resonance (NMR) spectroscopy and metabolic biochemistry studies suggest that closer coupling of energy demand and energy supply in Quechuas allows given changes in work rate with relatively modest changes in muscle adenylate and phosphagen concentrations, thus tempering the activation of glycolytic flux to pyruvate--a coarse control mechanism operating at the level of overall pathway flux. Later studies of enzyme activities in skeletal muscles of Quechuas and of Sherpas have identified a finely-tuned control mechanism which by adaptive modifications of a few key enzymes apparently serves to specifically attenuate pyruvate flux to lactate.

Novel retinoblastoma treatment avoids chemotherapy: the effect of optimally timed combination therapy with angiogenic and glycolytic inhibitors on LHBETATAG retinoblastoma tumors

Directory of Open Access Journals (Sweden)

Samuel K Houston

2011-01-01

Full Text Available Samuel K Houston1, Yolanda Piña1, Timothy G Murray1, Hinda Boutrid1, Colleen Cebulla2, Amy C Schefler1, Wei Shi1, Magda Celdran1, William Feuer1, Jaime Merchan3, Ted J Lampidis41Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Ophthalmology, The Ohio State University, Columbus, OH, USA; 3Division of Hematology/Oncology, Department of Medicine, 4Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine and Sylvester Comprehensive Cancer Center, Miami, FL, USAPurpose: The purpose of this study was to evaluate the effect of optimally timed combination treatment with angiogenic and glycolytic inhibitors on tumor burden, hypoxia, and angiogenesis in advanced retinoblastoma tumors.Methods: LHBETATAG mice (n = 30 were evaluated. Mice were divided into 5 groups (n = 6 and received injections at 16 weeks of age (advanced tumors with a saline, b anecortave acetate (AA, c 2-deoxyglucose (2-DG, d AA + 2-DG (1 day post-AA treatment, or e AA + 2-DG (1 week post-AA treatment. Eyes were enucleated at 21 weeks and tumor sections were analyzed for hypoxia, angiogenesis, and tumor burden.Results: Eyes treated with 2-DG 1 day post-AA injection showed a 23% (P = 0.03 reduction in tumor burden compared with 2-DG alone and a 61% (P < 0.001 reduction compared with saline-treated eyes. Eyes treated with 2-DG 1 week post-AA injection showed no significant decrease in tumor burden compared with 2-DG alone (P = 0.21 and a 56% (P < 0.001 decrease in comparison with saline-treated eyes. 2-DG significantly reduced the total density of new blood vessels in tumors by 44% compared to saline controls (P < 0.001, but did not affect the density of mature vasculature.Conclusions: Combination therapy with angiogenic and glycolytic inhibitors significantly enhanced tumor control. Synergistic effects were shown to be dependent on the temporal course of treatment

Serdemetan antagonizes the Mdm2-HIF1α axis leading to decreased levels of glycolytic enzymes.

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Jason A Lehman

Full Text Available Serdemetan (JNJ-26854165, an antagonist to Mdm2, was anticipated to promote the activation of p53. While regulation of p53 by Mdm2 is important, Mdm2 also regulates numerous proteins involved in diverse cellular functions. We investigated if Serdemetan would alter the Mdm2-HIF1α axis and affect cell survival in human glioblastoma cells independently of p53. Treatment of cells with Serdemetan under hypoxia resulted in a decrease in HIF1α levels. HIF1α downstream targets, VEGF and the glycolytic enzymes (enolase, phosphoglycerate kinase1/2, and glucose transporter 1, were all decreased in response to Serdemetan. The involvement of Mdm2 in regulating gene expression of glycolytic enzymes raises the possibility of side effects associated with therapeutically targeting Mdm2.

Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

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Chinatsu Mukai

Full Text Available Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase. We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.

Science.gov (United States)

Mukai, Chinatsu; Gao, Lizeng; Bergkvist, Magnus; Nelson, Jacquelyn L; Hinchman, Meleana M; Travis, Alexander J

2013-01-01

Maintaining activity of enzymes tethered to solid interfaces remains a major challenge in developing hybrid organic-inorganic devices. In nature, mammalian spermatozoa have overcome this design challenge by having glycolytic enzymes with specialized targeting domains that enable them to function while tethered to a cytoskeletal element. As a step toward designing a hybrid organic-inorganic ATP-generating system, we implemented a biomimetic site-specific immobilization strategy to tether two glycolytic enzymes representing different functional enzyme families: triose phosphoisomerase (TPI; an isomerase) and glyceraldehyde 3-phosphate dehydrogenase (GAPDHS; an oxidoreductase). We then evaluated the activities of these enzymes in comparison to when they were tethered via classical carboxyl-amine crosslinking. Both enzymes show similar surface binding regardless of immobilization method. Remarkably, specific activities for both enzymes were significantly higher when tethered using the biomimetic, site-specific immobilization approach. Using this biomimetic approach, we tethered both enzymes to a single surface and demonstrated their function in series in both forward and reverse directions. Again, the activities in series were significantly higher in both directions when the enzymes were coupled using this biomimetic approach versus carboxyl-amine binding. Our results suggest that biomimetic, site-specific immobilization can provide important functional advantages over chemically specific, but non-oriented attachment, an important strategic insight given the growing interest in recapitulating entire biological pathways on hybrid organic-inorganic devices.

A dynamo theory prediction for solar cycle 22: Sunspot number, radio flux, exospheric temperature, and total density at 400 km

Science.gov (United States)

Schatten, K. H.; Hedin, A. E.

1986-01-01

Using the dynamo theory method to predict solar activity, a value for the smoothed sunspot number of 109 + or - 20 is obtained for solar cycle 22. The predicted cycle is expected to peak near December, 1990 + or - 1 year. Concommitantly, F(10.7) radio flux is expected to reach a smoothed value of 158 + or - 18 flux units. Global mean exospheric temperature is expected to reach 1060 + or - 50 K and global total average total thermospheric density at 400 km is expected to reach 4.3 x 10 to the -15th gm/cu cm + or - 25 percent.

A dynamo theory prediction for solar cycle 22 - Sunspot number, radio flux, exospheric temperature, and total density at 400 km

Science.gov (United States)

Schatten, K. H.; Hedin, A. E.

1984-01-01

Using the 'dynamo theory' method to predict solar activity, a value for the smoothed sunspot number of 109 + or - 20 is obtained for solar cycle 22. The predicted cycle is expected to peak near December, 1990 + or - 1 year. Concommitantly, F(10.7) radio flux is expected to reach a smoothed value of 158 + or - 18 flux units. Global mean exospheric temperature is expected to reach 1060 + or - 50 K and global total average total thermospheric density at 400 km is expected to reach 4.3 x 10 to the -15th gm/cu cm + or - 25 percent.

Total Reflection X-ray Fluorescence Analysis (TXRF) using the high flux SAXS camera

CERN Document Server

Wobrauschek, P; Pepponi, G; Bergmann, A; Glatter, O

2002-01-01

Combining the high photon flux from a rotating anode X-ray tube with an X-ray optical component to focus and monochromatize the X-ray beam is the most promising instrumentation for best detection limits in the modern XRF laboratory. This is realized by using the design of a high flux SAXS camera in combination with a 4 kW high brilliant rotating Cu anode X-ray tube with a graded elliptically bent multilayer and including a new designed module for excitation in total reflection geometry within the beam path. The system can be evacuated thus reducing absorption and scattering of air and removing the argon peak in the spectra. Another novelty is the use of a Peltier cooled drift detector with an energy resolution of 148 eV at 5.9 keV and 5 mm sup 2 area. For Co detection limits of about 300 fg determined by a single element standard have been achieved. Testing a real sample NIST 1643d led to detection limits in the range of 300 ng/l for the medium Z.

Induced groundwater flux by increases in the aquifer's total stress.

Science.gov (United States)

Chang, Ching-Min; Yeh, Hund-Der

2015-01-01

Fluid-filled granular soils experience changes in total stress because of earth and oceanic tides, earthquakes, erosion, sedimentation, and changes in atmospheric pressure. The pore volume may deform in response to the changes in stress and this may lead to changes in pore fluid pressure. The transient fluid flow can therefore be induced by the gradient in excess pressure in a fluid-saturated porous medium. This work demonstrates the use of stochastic methodology in prediction of induced one-dimensional field-scale groundwater flow through a heterogeneous aquifer. A closed-form of mean groundwater flux is developed to quantify the induced field-scale mean behavior of groundwater flow and analyze the impacts of the spatial correlation length scale of log hydraulic conductivity and the pore compressibility. The findings provided here could be useful for the rational planning and management of groundwater resources in aquifers that contain lenses with large vertical aquifer matrix compressibility values. © 2014, National Ground Water Association.

Estimating the Total Heat Flux from the ASHES Hydrothermal Vent Field Using the Sentry Autonomous Underwater Vehicle

Science.gov (United States)

Crone, T. J.; Kinsey, J. C.; Mittelstaedt, E. L.

2017-12-01

Hydrothermal venting at mid-ocean ridges influences ocean chemistry, the thermal and chemical structure of the oceanic crust, and the evolution of unique and diverse autolithotrophically-supported ecosystems. Axially-hosted hydrothermal systems are responsible for 20-25% of the total heat flux out of Earth's interior, and likely play a large role in local as well as global biogeochemical cycles. Despite the importance of these systems, only a few studies have attempted to constrain the volume and heat flux of an entire hydrothermal vent field. In July of 2014 we used the Sentry autonomous underwater vehicle (AUV) to survey the water column over the ASHES hydrothermal vent field which is located within the caldera of Axial Seamount, an active submarine volcano located on the Juan de Fuca Ridge. To estimate the total heat and mass flux from this vent field, we equipped Sentry with a Nortek acoustic Doppler velocimeter (ADV), an inertial measurement unit (IMU), two acoustic Doppler current profilers (ADCPs), and two SBE3 temperature probes, allowing us to obtain precise measurements of fluid temperature and water velocity. The survey was designed using a control volume approach in which Sentry was pre-programmed to survey a 150-m-square centered over the vent field flying a grid pattern with 5-m track line spacing followed by a survey of the perimeter. This pattern was repeated multiple times during several 10-h dives at different altitudes, including 10, 20, 40, and 60 m above the seafloor, and during one 40-h survey at an altitude of 10 m. During the 40-h survey, the pattern was repeated nine times allowing us to obtain observations over several tidal cycles. Water velocity data obtained with Sentry were corrected for platform motion and then combined with the temperature measurements to estimate heat flux. The analysis of these data will likely provide the most accurate and highest resolution heat and mass flux estimates at a seafloor hydrothermal field to date.

Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus

Science.gov (United States)

Zhang, Yuehui; Sun, Xue; Sun, Xiaoyan; Meng, Fanci; Hu, Min; Li, Xin; Li, Wei; Wu, Xiao-Ke; Brännström, Mats; Shao, Ruijin; Billig, Håkan

2016-01-01

Peripheral insulin resistance and hyperandrogenism are the primary features of polycystic ovary syndrome (PCOS). However, how insulin resistance and hyperandrogenism affect uterine function and contribute to the pathogenesis of PCOS are open questions. We treated rats with insulin alone or in combination with human chorionic gonadotropin (hCG) and showed that peripheral insulin resistance and hyperandrogenism alter uterine morphology, cell phenotype, and cell function, especially in glandular epithelial cells. These defects are associated with an aberration in the PI3K/Akt signaling pathway that is used as an indicator for the onset of insulin resistance in classical metabolic tissues. Concomitantly, increased GSK3β (Ser-9) phosphorylation and decreased ERK1/2 phosphorylation in rats treated with insulin and hCG were also observed. We also profiled the expression of glucose transporter (Glut) isoform genes in the uterus under conditions of insulin resistance and/or hyperandrogenism. Finally, we determined the expression pattern of glycolytic enzymes and intermediates during insulin resistance and hyperandrogenism in the uterus. These findings suggest that the PI3K/Akt and MAPK/ERK signaling pathways play a role in the onset of uterine insulin resistance, and they also suggest that changes in specific Glut isoform expression and alterations to glycolytic metabolism contribute to the endometrial dysfunction observed in PCOS patients. PMID:27461373

Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation.

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Christian Berrios

2016-11-01

Full Text Available Merkel cell polyomavirus (MCPyV is an etiological agent of Merkel cell carcinoma (MCC, a highly aggressive skin cancer. The MCPyV small tumor antigen (ST is required for maintenance of MCC and can transform normal cells. To gain insight into cellular perturbations induced by MCPyV ST, we performed transcriptome analysis of normal human fibroblasts with inducible expression of ST. MCPyV ST dynamically alters the cellular transcriptome with increased levels of glycolytic genes, including the monocarboxylate lactate transporter SLC16A1 (MCT1. Extracellular flux analysis revealed increased lactate export reflecting elevated aerobic glycolysis in ST expressing cells. Inhibition of MCT1 activity suppressed the growth of MCC cell lines and impaired MCPyV-dependent transformation of IMR90 cells. Both NF-κB and MYC have been shown to regulate MCT1 expression. While MYC was required for MCT1 induction, MCPyV-induced MCT1 levels decreased following knockdown of the NF-κB subunit RelA, supporting a synergistic activity between MCPyV and MYC in regulating MCT1 levels. Several MCC lines had high levels of MYCL and MYCN but not MYC. Increased levels of MYCL was more effective than MYC or MYCN in increasing extracellular acidification in MCC cells. Our results demonstrate the effects of MCPyV ST on the cellular transcriptome and reveal that transformation is dependent, at least in part, on elevated aerobic glycolysis.

Upregulation of glycolytic enzymes, mitochondrial dysfunction and increased cytotoxicity in glial cells treated with Alzheimer's disease plasma.

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Tharusha Jayasena

Full Text Available Alzheimer's disease (AD is a neurodegenerative disorder associated with increased oxidative stress and neuroinflammation. Markers of increased protein, lipid and nucleic acid oxidation and reduced activities of antioxidant enzymes have been reported in AD plasma. Amyloid plaques in the AD brain elicit a range of reactive inflammatory responses including complement activation and acute phase reactions, which may also be reflected in plasma. Previous studies have shown that human AD plasma may be cytotoxic to cultured cells. We investigated the effect of pooled plasma (n = 20 each from healthy controls, individuals with amnestic mild cognitive impairment (aMCI and Alzheimer's disease (AD on cultured microglial cells. AD plasma and was found to significantly decrease cell viability and increase glycolytic flux in microglia compared to plasma from healthy controls. This effect was prevented by the heat inactivation of complement. Proteomic methods and isobaric tags (iTRAQ found the expression level of complement and other acute phase proteins to be altered in MCI and AD plasma and an upregulation of key enzymes involved in the glycolysis pathway in cells exposed to AD plasma. Altered expression levels of acute phase reactants in AD plasma may alter the energy metabolism of glia.

Effects of Interval Training-Based Glycolytic Capacity on Physical Fitness in Recreational Long-Distance Runners

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Zatoń Marek

2015-06-01

Full Text Available Purpose. The aim of this study was to investigate the influence of 8-week-long interval training (targeting glycolytic capacity on selected markers of physical fitness in amateur long-distance runners. Methods. The study involved 17 amateur long-distance runners randomly divided into an experimental (n = 8 and control (n = 9 group. The control group performed three or four continuous training sessions per week whereas the experimental group performed two interval running training sessions and one continuous running training session. A graded treadmill exercise test and the 12-min Cooper test were performed pre- and post-training. Results. O2max and the rate of recovery increased in the experimental group. Relative oxygen uptake, minute ventilation, and heart rate speed decreased in low- (6 km/h and medium-intensity (12 km/h running. Conclusions. Both training modalities showed similar results. However, the significant differences in training volume (4-8 min interval training vs. 40-150 min continuous training indicates that the modalities targeting glycolytic capacity may be more efficient for amateur runners prepare for long-distance events.

CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.

Science.gov (United States)

Le Floch, Renaud; Chiche, Johanna; Marchiq, Ibtissam; Naiken, Tanesha; Naïken, Tanesha; Ilc, Karine; Ilk, Karine; Murray, Clare M; Critchlow, Susan E; Roux, Danièle; Simon, Marie-Pierre; Pouysségur, Jacques

2011-10-04

Malignant tumors exhibit increased dependence on glycolysis, resulting in abundant export of lactic acid, a hypothesized key step in tumorigenesis. Lactic acid is mainly transported by two H(+)/lactate symporters, MCT1/MCT4, that require the ancillary protein CD147/Basigin for their functionality. First, we showed that blocking MCT1/2 in Ras-transformed fibroblasts with AR-C155858 suppressed lactate export, glycolysis, and tumor growth, whereas ectopic expression of MCT4 in these cells conferred resistance to MCT1/2 inhibition and reestablished tumorigenicty. A mutant-derivative, deficient in respiration (res(-)) and exclusively relying on glycolysis for energy, displayed low tumorigenicity. These res(-) cells could develop resistance to MCT1/2 inhibition and became highly tumorigenic by reactivating their endogenous mct4 gene, highlighting that MCT4, the hypoxia-inducible and tumor-associated lactate/H(+) symporter, drives tumorigenicity. Second, in the human colon adenocarcinoma cell line (LS174T), we showed that combined silencing of MCT1/MCT4 via inducible shRNA, or silencing of CD147/Basigin alone, significantly reduced glycolytic flux and tumor growth. However, both silencing approaches, which reduced tumor growth, displayed a low level of CD147/Basigin, a multifunctional protumoral protein. To gain insight into CD147/Basigin function, we designed experiments, via zinc finger nuclease-mediated mct4 and basigin knockouts, to uncouple MCTs from Basigin expression. Inhibition of MCT1 in MCT4-null, Basigin(high) cells suppressed tumor growth. Conversely, in Basigin-null cells, in which MCT activity had been maintained, tumorigenicity was not affected. Collectively, these findings highlight that the major protumoral action of CD147/Basigin is to control the energetics of glycolytic tumors via MCT1/MCT4 activity and that blocking lactic acid export provides an efficient anticancer strategy.

Studies on cell-free metabolism: ethanol production by a yeast glycolytic system reconstituted from purified enzymes

Energy Technology Data Exchange (ETDEWEB)

Welch, P; Scopes, R K

1985-07-01

A reconstituted glycolytic system has been established from individually purified enzymes to simulate the conversion of glucose to ethanol plus CO/sub 2/ by yeast. Sustained and extensive conversion occurred provided that input of glucose matched the rate of ATP degradation appropriately. ATPase activity could be replaced by arsenate, which uncoupled ATP synthesis from glycolysis. The mode of uncoupling was investigated, and it was concluded that the artificial intermediate, 1-arseno-3-phosphoglycerate, has a half-life of no more than a few milliseconds. Arsenate at 4 mM concentration could simulate the equivalent of 10 ..mu..mol/ml min. of ATPase activity. The reconstituted enzyme system was capable of totally degrading one M (18% w/v) glucose in 8 hours giving 9% (w/v) ethanol. The levels of metabolites during metabolism were measured to detect rate-limiting steps. The successful operation of the reconstituted enzyme system demonstrates that it is possible to carry out complex chemical transformations with multiple enzyme systems in vitro. 36 references.

Total luminous flux measurement for flexible surface sources with an integrating sphere photometer

International Nuclear Information System (INIS)

Yu, Hsueh-Ling; Liu, Wen-Chun

2014-01-01

Applying an integrating sphere photometer for total luminous flux measurement is a widely used method. However, the measurement accuracy depends on the spatial uniformity of the integrating sphere, especially when the test sample has a different light distribution from that of the standard source. Therefore, spatial correction is needed to eliminate the effect caused by non-uniformity. To reduce the inconvenience of spatial correction but retain the measurement accuracy, a new type of working standard is designed for flexible and curved surface sources. Applying this new type standard source, the measurement deviation due to different orientations is reduced by an order of magnitude compared with using a naked incandescent lamp as the standard source. (paper)

A chemometric method to identify enzymatic reactions leading to the transition from glycolytic oscillations to waves

Science.gov (United States)

Zimányi, László; Khoroshyy, Petro; Mair, Thomas

2010-06-01

In the present work we demonstrate that FTIR-spectroscopy is a powerful tool for the time resolved and noninvasive measurement of multi-substrate/product interactions in complex metabolic networks as exemplified by the oscillating glycolysis in a yeast extract. Based on a spectral library constructed from the pure glycolytic intermediates, chemometric analysis of the complex spectra allowed us the identification of many of these intermediates. Singular value decomposition and multiple level wavelet decomposition were used to separate drifting substances from oscillating ones. This enabled us to identify slow and fast variables of glycolytic oscillations. Most importantly, we can attribute a qualitative change in the positive feedback regulation of the autocatalytic reaction to the transition from homogeneous oscillations to travelling waves. During the oscillatory phase the enzyme phosphofructokinase is mainly activated by its own product ADP, whereas the transition to waves is accompanied with a shift of the positive feedback from ADP to AMP. This indicates that the overall energetic state of the yeast extract determines the transition between spatially homogeneous oscillations and travelling waves.

MCT1-mediated transport of a toxic molecule is an effective strategy for targeting glycolytic tumors

Science.gov (United States)

Birsoy, Kivanc; Wang, Tim; Possemato, Richard; Yilmaz, Omer H.; Koch, Catherine E.; Chen, Walter W.; Hutchins, Amanda W.; Gultekin, Yetis; Peterson, Tim R.; Carette, Jan E.; Brummelkamp, Thijn R.; Clish, Clary B.; Sabatini, David M.

2012-01-01

SUMMARY There is increasing evidence that oncogenic transformation modifies the metabolic program of cells. A common alteration is the upregulation of glycolysis, and efforts to target glycolytic enzymes for anti-cancer therapy are underway. Here, we performed a genome-wide haploid genetic screen to identify resistance mechanisms to 3-bromopyruvate (3-BrPA), a drug candidate that inhibits glycolysis in a poorly understood fashion. We identified the SLC16A1 gene product, MCT1, as the main determinant of 3-BrPA sensitivity. MCT1 is necessary and sufficient for 3-BrPA uptake by cancer cells. Additionally, MCT1 mRNA levels are the best predictor of 3-BrPA sensitivity and are most elevated in glycolytic cancer cells. Lastly, forced MCT1 expression in 3-BrPA resistant cancer cells sensitizes tumor xenografts to 3-BrPA treatment in vivo. Our results identify a potential biomarker for 3-BrPA sensitivity and provide proof of concept that the selectivity of cancer-expressed transporters can be exploited for delivering toxic molecules to tumors. PMID:23202129

Effect of hypoxia on the activity and binding of glycolytic and associated enzymes in sea scorpion tissues

Directory of Open Access Journals (Sweden)

Lushchak V.I.

1998-01-01

Full Text Available The effect of hypoxia on the levels of glycogen, glucose and lactate as well as the activities and binding of glycolytic and associated enzymes to subcellular structures was studied in brain, liver and white muscle of the teleost fish, Scorpaena porcus. Hypoxia exposure decreased glucose levels in liver from 2.53 to 1.70 µmol/g wet weight and in muscle led to its increase from 3.64 to 25.1 µmol/g wet weight. Maximal activities of several enzymes in brain were increased by hypoxia: hexokinase by 23%, phosphoglucoisomerase by 47% and phosphofructokinase (PFK by 56%. However, activities of other enzymes in brain as well as enzymes in liver and white muscle were largely unchanged or decreased during experimental hypoxia. Glycolytic enzymes in all three tissues were partitioned between soluble and particulate-bound forms. In several cases, the percentage of bound enzymes was reduced during hypoxia; bound aldolase in brain was reduced from 36.4 to 30.3% whereas glucose-6-phosphate dehydrogenase fell from 55.7 to 28.7% bound. In muscle PFK was reduced from 57.4 to 41.7% bound. Oppositely, the proportion of bound aldolase and triosephosphate isomerase increased in hypoxic muscle. Phosphoglucomutase did not appear to occur in a bound form in liver and bound phosphoglucomutase disappeared in muscle during hypoxia exposure. Anoxia exposure also led to the disappearance of bound fructose-1,6-bisphosphatase in liver, whereas a bound fraction of this enzyme appeared in white muscle of anoxic animals. The possible function of reversible binding of glycolytic enzymes to subcellular structures as a regulatory mechanism of carbohydrate metabolism is discussed.

Glycolytic enzyme activity is essential for domestic cat (Felis catus) and cheetah (Acinonyx jubatus) sperm motility and viability in a sugar-free medium.

Science.gov (United States)

Terrell, Kimberly A; Wildt, David E; Anthony, Nicola M; Bavister, Barry D; Leibo, S P; Penfold, Linda M; Marker, Laurie L; Crosier, Adrienne E

2011-06-01

We have previously reported a lack of glucose uptake in domestic cat and cheetah spermatozoa, despite observing that these cells produce lactate at rates that correlate positively with sperm function. To elucidate the role of glycolysis in felid sperm energy production, we conducted a comparative study in the domestic cat and cheetah, with the hypothesis that sperm motility and viability are maintained in both species in the absence of glycolytic metabolism and are fueled by endogenous substrates. Washed ejaculates were incubated in chemically defined medium in the presence/absence of glucose and pyruvate. A second set of ejaculates was exposed to a chemical inhibitor of either lactate dehydrogenase (sodium oxamate) or glyceraldehyde-3-phosphate dehydrogenase (alpha-chlorohydrin). Sperm function (motility and acrosomal integrity) and lactate production were assessed, and a subset of spermatozoa was assayed for intracellular glycogen. In both the cat and cheetah, sperm function was maintained without exogenous substrates and following lactate dehydrogenase inhibition. Lactate production occurred in the absence of exogenous hexoses, but only if pyruvate was present. Intracellular glycogen was not detected in spermatozoa from either species. Unexpectedly, glycolytic inhibition by alpha-chlorohydrin resulted in an immediate decline in sperm motility, particularly in the domestic cat. Collectively, our findings reveal an essential role of the glycolytic pathway in felid spermatozoa that is unrelated to hexose metabolism or lactate formation. Instead, glycolytic enzyme activity could be required for the metabolism of endogenous lipid-derived glycerol, with fatty acid oxidation providing the primary energy source in felid spermatozoa.

Phosphatase control of 4E-BP1 phosphorylation state is central for glycolytic regulation of retinal protein synthesis.

Science.gov (United States)

Gardner, Thomas W; Abcouwer, Steven F; Losiewicz, Mandy K; Fort, Patrice E

2015-09-15

Control of protein synthesis in insulin-responsive tissues has been well characterized, but relatively little is known about how this process is regulated in nervous tissues. The retina exhibits a relatively high protein synthesis rate, coinciding with high basal Akt and metabolic activities, with the majority of retinal ATP being derived from aerobic glycolysis. We examined the dependency of retinal protein synthesis on the Akt-mTOR signaling and glycolysis using ex vivo rat retinas. Akt inhibitors significantly reduced retinal protein synthesis but did not affect glycolytic lactate production. Surprisingly, the glycolytic inhibitor 2-deoxyglucose (2-DG) markedly inhibited Akt1 and Akt3 activities, as well as protein synthesis. The effects of 2-DG, and 2-fluorodeoxyglucose (2-FDG) on retinal protein synthesis correlated with inhibition of lactate production and diminished ATP content, with all these effects reversed by provision of d-mannose. 2-DG treatment was not associated with increased AMPK, eEF2, or eIF2α phosphorylation; instead, it caused rapid dephosphorylation of 4E-BP1. 2-DG reduced total mTOR activity by 25%, but surprisingly, it did not reduce mTORC1 activity, as indicated by unaltered raptor-associated mTOR autophosphorylation and ribosomal protein S6 phosphorylation. Dephosphorylation of 4E-BP1 was largely prevented by inhibition of PP1/PP2A phosphatases with okadaic acid and calyculin A, and inhibition of PPM1 phosphatases with cadmium. Thus, inhibition of retinal glycolysis diminished Akt and protein synthesis coinciding with accelerated dephosphorylation of 4E-BP1 independently of mTORC1. These results demonstrate a novel mechanism regulating protein synthesis in the retina involving an mTORC1-independent and phosphatase-dependent regulation of 4E-BP1. Copyright © 2015 the American Physiological Society.

Carbohydrate metabolism of Xylella fastidiosa: Detection of glycolytic and pentose phosphate pathway enzymes and cloning and expression of the enolase gene

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Facincani Agda Paula

2003-01-01

Full Text Available The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor, Triton X-100, and TCA. Enolase extracted from X. fastidiosa and from chicken muscle and liver is irreversibly inactivated by urea. The purification of enolase was partial and resulted in a low yield. No enzymatic activity was detected for either recombinant and native enolases, aldolase, and glyceraldehyde-3-phosphate dehydrogenase, suggesting that X. fastidiosa uses the Entner-Doudoroff pathway to produce pyruvate. Evidence is presented supporting the idea that the regulation of genes and the presence of isoforms with regulation patterns might make it difficult to understand the metabolism of carbohydrates in X. fastidiosa.

POLAMI: Polarimetric Monitoring of Active Galactic Nuclei at Millimetre Wavelengths - III. Characterization of total flux density and polarization variability of relativistic jets

Science.gov (United States)

Agudo, Iván; Thum, Clemens; Ramakrishnan, Venkatessh; Molina, Sol N.; Casadio, Carolina; Gómez, José L.

2018-01-01

We report on the first results of the POLAMI (Polarimetric Monitoring of AGNs with Millimetre Wavelengths) programme, a simultaneous 3.5 and 1.3 mm full-Stokes-polarization monitoring of a sample of 36 of the brightest active galactic nuclei in the northern sky with the IRAM 30 m telescope. Through a systematic statistical study of data taken from 2006 October (from 2009 December for the case of the 1.3 mm observations) to 2014 August, we characterize the variability of the total flux density and linear polarization. We find that all sources in the sample are highly variable in total flux density at both 3.5 and 1.3 mm, as well as in spectral index, which (except in particularly prominent flares) is found to be optically thin between these two wavelengths. The total flux-density variability at 1.3 mm is found, in general, to be faster, and to have larger fractional amplitude and flatter power-spectral-density slopes than at 3.5 mm. The polarization degree is on average larger at 1.3 mm than at 3.5 mm, by a factor of 2.6. The variability of linear polarization degree is faster and has higher fractional amplitude than for total flux density, with the typical time-scales during prominent polarization peaks being significantly faster at 1.3 mm than at 3.5 mm. The polarization angle at both 3.5 and 1.3 mm is highly variable. Most of the sources show one or two excursions of >180° on time-scales from a few weeks to about a year during the course of our observations. The 3.5 and 1.3 mm polarization angle evolution follows each other rather well, although the 1.3 mm data show a clear preference to more prominent variability on the short time-scales, i.e. weeks. The data are compatible with multizone models of conical jets involving smaller emission regions for the shortest-wavelength emitting sites. Such smaller emitting regions should also be more efficient in energising particle populations, as implied by the coherent evolution of the spectral index and the total flux

Study of the Induction Machine Unsymmetrical Condition Using In Total Fluxes Equations

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SIMION, A.

2010-02-01

Full Text Available On the basis of the mathematical model, called in total fluxes in a previous paper, and which is proper for the analysis of transient operation of the two-phase induction machine, one obtains the symmetrical steady-state equations, which are valid for three-phase machines, as well. The obtained mathematical expressions are much more simple and easier to use than the consecrated ones, which are generally applied in scientific literature. Moreover, considerations are to be made upon the space-time rotational vectors, emphasizing their importance in understanding the physical phenomena that characterize induction machines. The use of these space vectors is further tested out for the study of unsymmetrical supply, which gives a much faster method in obtaining the electromagnetic torque expression. Finally, the results are compared with the ones that come out from the traditional methods, more exactly, the symmetric component method.

An operon encoding three glycolytic enzymes in Lactobacillus delbrueckii subsp. bulgaricus: glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase.

Science.gov (United States)

Branny, P; de la Torre, F; Garel, J R

1998-04-01

The structural genes gap, pgk and tpi encoding three glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TPI), respectively, have been cloned and sequenced from Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The genes were isolated after screening genomic sublibraries with specific gap and pgk probes obtained by PCR amplification of chromosomal DNA with degenerate primers corresponding to amino acid sequences highly conserved in GAPDHs and PGKs. Nucleotide sequencing revealed that the three genes were organized in the order gap-pgk-tpi. The translation start codons of the three genes were identified by alignment of the N-terminal sequences. These genes predicted polypeptide chains of 338, 403 and 252 amino acids for GAPDH, PGK and TPI, respectively, and they were separated by 96 bp between gap and pgk, and by only 18 bp between pgk and tpi. The codon usage in gap, pgk, tpi and three other glycolytic genes from L. bulgaricus differed, noticeably from that in other chromosomal genes. The site of transcriptional initiation was located by primer extension, and a probable promoter was identified for the gap-pgk-tpi operon. Northern hybridization of total RNA with specific probes showed two transcripts, an mRNA of 1.4 kb corresponding to the gap gene, and a less abundant mRNA of 3.4 kb corresponding to the gap-pgk-tpi cluster. The absence of a visible terminator in the 3'-end of the shorter transcript and the location of this 3'-end inside the pgk gene indicated that this shorter transcript was produced by degradation of the longer one, rather than by an early termination of transcription after the gap gene.

Experimental determination of control of glycolysis in Lactococcus lactis

DEFF Research Database (Denmark)

Købmann, Brian Jensen; Andersen, Heidi Winterberg; Solem, Christian

2002-01-01

), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PYK) and lactate dehydrogenase (LDH) are shown to have no significant control on the glycolytic flux in exponentially growing cells of L. lactis MG1363. Introduction of an uncoupled ATPase activity results in uncoupling of glycolysis from biomass...... production. With MG1363 growing in defined medium supplemented with glucose, the ATP demanding processes do not have a significant control on the glycolytic flux; it appears that glycolysis is running at maximal rate. It is likely that the flux control is distributed over many enzymes in L. lactis...

Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors

DEFF Research Database (Denmark)

Johansen, Torben

1979-01-01

The adenosine triphosphate (ATP) content of rat mast cells was studied during and after anaphylactic histamine release. The almost identical time course of ATP decrease from mast cells treated with either glycolytic or respiratory inhibitors supports the view that the ATP depletion was largely re...

Glycogen Shunt Activity and Glycolytic Supercompensation in Astrocytes May Be Distinctly Mediated via the Muscle Form of Glycogen Phosphorylase

DEFF Research Database (Denmark)

Jakobsen, Emil; Bak, Lasse K; Walls, Anne B

2017-01-01

Glycogen is the main storage form of glucose in the brain. In contrast with previous beliefs, brain glycogen has recently been shown to play important roles in several brain functions. A fraction of metabolized glucose molecules are being shunted through glycogen before reentering the glycolytic ...

uPA/uPAR system activation drives a glycolytic phenotype in melanoma cells.

Science.gov (United States)

Laurenzana, Anna; Chillà, Anastasia; Luciani, Cristina; Peppicelli, Silvia; Biagioni, Alessio; Bianchini, Francesca; Tenedini, Elena; Torre, Eugenio; Mocali, Alessandra; Calorini, Lido; Margheri, Francesca; Fibbi, Gabriella; Del Rosso, Mario

2017-09-15

In this manuscript, we show the involvement of the uPA/uPAR system in the regulation of aerobic glycolysis of melanoma cells. uPAR over-expression in human melanoma cells controls an invasive and glycolytic phenotype in normoxic conditions. uPAR down-regulation by siRNA or its uncoupling from integrins, and hence from integrin-linked tyrosine kinase receptors (IL-TKRs), by an antagonist peptide induced a striking inhibition of the PI3K/AKT/mTOR/HIF1α pathway, resulting into impairment of glucose uptake, decrease of several glycolytic enzymes and of PKM2, a checkpoint that controls metabolism of cancer cells. Further, binding of uPA to uPAR regulates expression of molecules that govern cell invasion, including extracellular matrix metallo-proteinases inducer (EMPPRIN) and enolase, a glycolytyc enzyme that also serves as a plasminogen receptor, thus providing a common denominator between tumor metabolism and phenotypic invasive features. Such effects depend on the α5β1-integrin-mediated uPAR connection with EGFR in melanoma cells with engagement of the PI3K-mTOR-HIFα pathway. HIF-1α trans-activates genes whose products mediate tumor invasion and glycolysis, thus providing the common denominator between melanoma metabolism and its invasive features. These findings unveil a unrecognized interaction between the invasion-related uPAR and IL-TKRs in the control of glycolysis and disclose a new pharmacological target (i.e., uPAR/IL-TKRs axis) for the therapy of melanoma. © 2017 UICC.

Potential role of the glycolytic oscillator in acute hypoxia in tumors

International Nuclear Information System (INIS)

Fru, Leonard Che; Adamson, Erin B; Campos, David D; Fain, Sean B; Song, Chihwa; Kissick, Michael W; Jacques, Steven L; Van der Kogel, Albert J; Nickel, Kwang P; Kimple, Randall J

2015-01-01

Tumor acute hypoxia has a dynamic component that is also, at least partially, coherent. Using blood oxygen level dependent magnetic resonance imaging, we observed coherent oscillations in hemoglobin saturation dynamics in cell line xenograft models of head and neck squamous cell carcinoma. We posit a well-established biochemical nonlinear oscillatory mechanism called the glycolytic oscillator as a potential cause of the coherent oscillations in tumors. These data suggest that metabolic changes within individual tumor cells may affect the local tumor microenvironment including oxygen availability and therefore radiosensitivity. These individual cells can synchronize the oscillations in patches of similar intermediate glucose levels. These alterations have potentially important implications for radiation therapy and are a potential target for optimizing the cancer response to radiation. (paper)

Determining the primary cosmic ray energy from the total flux of Cherenkov light measured at the Yakutsk EAS array

International Nuclear Information System (INIS)

Ivanov, A. A.; Knurenko, S. P.; Sleptsov, I. E.

2007-01-01

We present a method for determining the energy of the primary particle that generates an extensive air shower (EAS) of comic rays based on measuring the total flux of Cherenkov light from the shower. Applying this method to Cherenkov light measurements at the Yakutsk EAS array has allowed us to construct the cosmic ray energy spectrum in the range 10 15 - 3 x 10 19 eV

Fermentation of Xylose Causes Inefficient Metabolic State Due to Carbon/Energy Starvation and Reduced Glycolytic Flux in Recombinant Industrial Saccharomyces cerevisiae

Science.gov (United States)

Matsushika, Akinori; Nagashima, Atsushi; Goshima, Tetsuya; Hoshino, Tamotsu

2013-01-01

In the present study, comprehensive, quantitative metabolome analysis was carried out on the recombinant glucose/xylose-cofermenting S. cerevisiae strain MA-R4 during fermentation with different carbon sources, including glucose, xylose, or glucose/xylose mixtures. Capillary electrophoresis time-of-flight mass spectrometry was used to determine the intracellular pools of metabolites from the central carbon pathways, energy metabolism pathways, and the levels of twenty amino acids. When xylose instead of glucose was metabolized by MA-R4, glycolytic metabolites including 3- phosphoglycerate, 2- phosphoglycerate, phosphoenolpyruvate, and pyruvate were dramatically reduced, while conversely, most pentose phosphate pathway metabolites such as sedoheptulose 7- phosphate and ribulose 5-phosphate were greatly increased. These results suggest that the low metabolic activity of glycolysis and the pool of pentose phosphate pathway intermediates are potential limiting factors in xylose utilization. It was further demonstrated that during xylose fermentation, about half of the twenty amino acids declined, and the adenylate/guanylate energy charge was impacted due to markedly decreased adenosine triphosphate/adenosine monophosphate and guanosine triphosphate/guanosine monophosphate ratios, implying that the fermentation of xylose leads to an inefficient metabolic state where the biosynthetic capabilities and energy balance are severely impaired. In addition, fermentation with xylose alone drastically increased the level of citrate in the tricarboxylic acid cycle and increased the aromatic amino acids tryptophan and tyrosine, strongly supporting the view that carbon starvation was induced. Interestingly, fermentation with xylose alone also increased the synthesis of the polyamine spermidine and its precursor S-adenosylmethionine. Thus, differences in carbon substrates, including glucose and xylose in the fermentation medium, strongly influenced the dynamic metabolism of MA-R4

Genetic disruption of lactate/H+ symporters (MCTs) and their subunit CD147/BASIGIN sensitizes glycolytic tumor cells to phenformin.

Science.gov (United States)

Marchiq, Ibtissam; Le Floch, Renaud; Roux, Danièle; Simon, Marie-Pierre; Pouyssegur, Jacques

2015-01-01

Rapidly growing glycolytic tumors require energy and intracellular pH (pHi) homeostasis through the activity of two major monocarboxylate transporters, MCT1 and the hypoxia-inducible MCT4, in intimate association with the glycoprotein CD147/BASIGIN (BSG). To further explore and validate the blockade of lactic acid export as an anticancer strategy, we disrupted, via zinc finger nucleases, MCT4 and BASIGIN genes in colon adenocarcinoma (LS174T) and glioblastoma (U87) human cell lines. First, we showed that homozygous loss of MCT4 dramatically sensitized cells to the MCT1 inhibitor AZD3965. Second, we demonstrated that knockout of BSG leads to a decrease in lactate transport activity of MCT1 and MCT4 by 10- and 6-fold, respectively. Consequently, cells accumulated an intracellular pool of lactic and pyruvic acids, magnified by the MCT1 inhibitor decreasing further pHi and glycolysis. As a result, we found that these glycolytic/MCT-deficient cells resumed growth by redirecting their metabolism toward OXPHOS. Third, we showed that in contrast with parental cells, BSG-null cells became highly sensitive to phenformin, an inhibitor of mitochondrial complex I. Phenformin addition to these MCT-disrupted cells in normoxic and hypoxic conditions induced a rapid drop in cellular ATP-inducing cell death by "metabolic catastrophe." Finally, xenograft analysis confirmed the deleterious tumor growth effect of MCT1/MCT4 ablation, an action enhanced by phenformin treatment. Collectively, these findings highlight that inhibition of the MCT/BSG complexes alone or in combination with phenformin provides an acute anticancer strategy to target highly glycolytic tumors. This genetic approach validates the anticancer potential of the MCT1 and MCT4 inhibitors in current development. ©2014 American Association for Cancer Research.

Primary clear cell renal carcinoma cells display minimal mitochondrial respiratory capacity resulting in pronounced sensitivity to glycolytic inhibition by 3-Bromopyruvate.

Science.gov (United States)

Nilsson, H; Lindgren, D; Mandahl Forsberg, A; Mulder, H; Axelson, H; Johansson, M E

2015-01-08

Changes of cellular metabolism are an integral property of the malignant potential of most cancer cells. Already in the 1930s, Otto Warburg observed that tumor cells preferably utilize glycolysis and lactate fermentation for energy production, rather than the mitochondrial oxidative phosphorylation dominating in normal cells, a phenomenon today known as the Warburg effect. Even though many tumor types display a high degree of aerobic glycolysis, they still retain the activity of other energy-producing metabolic pathways. One exception seems to be the clear cell variant of renal cell carcinoma, ccRCC, where the activity of most other pathways than that of glycolysis has been shown to be reduced. This makes ccRCC a promising candidate for the use of glycolytic inhibitors in treatment of the disease. However, few studies have so far addressed this issue. In this report, we show a strikingly reduced mitochondrial respiratory capacity of primary human ccRCC cells, resulting in enhanced sensitivity to glycolytic inhibition by 3-Bromopyruvate (3BrPA). This effect was largely absent in established ccRCC cell lines, a finding that highlights the importance of using biologically relevant models in the search for new candidate cancer therapies. 3BrPA markedly reduced ATP production in primary ccRCC cells, followed by cell death. Our data suggest that glycolytic inhibitors such as 3BrPA, that has been shown to be well tolerated in vivo, should be further analyzed for the possible development of selective treatment strategies for patients with ccRCC.

Nitrous oxide fluxes from grassland in the Netherlands. 1. Statistical analysis of flux-chamber measurements

NARCIS (Netherlands)

Velthof, G.L.; Oenema, O.

1995-01-01

Accurate estimates of total nitrous oxide (N2O) losses from grasslands derived from flux-chamber measurements are hampered by the large spatial and temporal variability of N2O fluxes from these sites. In this study, four methods for the calculation o

Long term effect of curcumin in regulation of glycolytic pathway and angiogenesis via modulation of stress activated genes in prevention of cancer.

Directory of Open Access Journals (Sweden)

Laxmidhar Das

Full Text Available Oxidative stress, an important factor in modulation of glycolytic pathway and induction of stress activated genes, is further augmented due to reduced antioxidant defense system, which promotes cancer progression via inducing angiogenesis. Curcumin, a naturally occurring chemopreventive phytochemical, is reported to inhibit carcinogenesis in various experimental animal models. However, the underlying mechanism involved in anticarcinogenic action of curcumin due to its long term effect is still to be reported because of its rapid metabolism, although metabolites are accumulated in tissues and remain for a longer time. Therefore, the long term effect of curcumin needs thorough investigation. The present study aimed to analyze the anticarcinogenic action of curcumin in liver, even after withdrawal of treatment in Dalton's lymphoma bearing mice. Oxidative stress observed during lymphoma progression reduced antioxidant enzyme activities, and induced angiogenesis as well as activation of early stress activated genes and glycolytic pathway. Curcumin treatment resulted in activation of antioxidant enzyme super oxide dismutase and down regulation of ROS level as well as activity of ROS producing enzyme NADPH:oxidase, expression of stress activated genes HIF-1α, cMyc and LDH activity towards normal level. Further, it lead to significant inhibition of angiogenesis, observed via MMPs activity, PKCα and VEGF level, as well as by matrigel plug assay. Thus findings of this study conclude that the long term effect of curcumin shows anticarcinogenic potential via induction of antioxidant defense system and inhibition of angiogenesis via down regulation of stress activated genes and glycolytic pathway in liver of lymphoma bearing mice.

Turbulent transport across invariant canonical flux surfaces

International Nuclear Information System (INIS)

Hollenberg, J.B.; Callen, J.D.

1994-07-01

Net transport due to a combination of Coulomb collisions and turbulence effects in a plasma is investigated using a fluid moment description that allows for kinetic and nonlinear effects via closure relations. The model considered allows for ''ideal'' turbulent fluctuations that distort but preserve the topology of species-dependent canonical flux surfaces ψ number-sign,s triple-bond ∫ dF · B number-sign,s triple-bond ∇ x [A + (m s /q s )u s ] in which u s is the flow velocity of the fluid species. Equations for the net transport relative to these surfaces due to ''nonideal'' dissipative processes are found for the total number of particles and total entropy enclosed by a moving canonical flux surface. The corresponding particle transport flux is calculated using a toroidal axisymmetry approximation of the ideal surfaces. The resulting Lagrangian transport flux includes classical, neoclassical-like, and anomalous contributions and shows for the first time how these various contributions should be summed to obtain the total particle transport flux

Modulation of cellular radiation responses by 2-deoxy-D-glucose and other glycolytic inhibitors: Implications for cancer therapy

OpenAIRE

Kalia Vijay; Prabhakara S; Narayanan Vidya

2009-01-01

Background: 2-Deoxy-D-glucose (2-DG), a glycolytic inhibitor, was observed earlier to increase DNA, chromosomal, and cellular damage in tumor cells, by inhibiting energy-dependent repair processes. Lonidamine (LND) selectively inhibits glycolysis in cancer cells. It damages the condensed mitochondria in these cells, impairing thereby the activity of hexokinase (predominantly attached to the outer mitochondrial membranes). It inhibits repair of radiation-induced potentially lethal cellular da...

Local and global bifurcations at infinity in models of glycolytic oscillations

DEFF Research Database (Denmark)

Sturis, Jeppe; Brøns, Morten

1997-01-01

We investigate two models of glycolytic oscillations. Each model consists of two coupled nonlinear ordinary differential equations. Both models are found to have a saddle point at infinity and to exhibit a saddle-node bifurcation at infinity, giving rise to a second saddle and a stable node...... at infinity. Depending on model parameters, a stable limit cycle may blow up to infinite period and amplitude and disappear in the bifurcation, and after the bifurcation, the stable node at infinity then attracts all trajectories. Alternatively, the stable node at infinity may coexist with either a stable...... sink (not at infinity) or a stable limit cycle. This limit cycle may then disappear in a heteroclinic bifurcation at infinity in which the unstable manifold from one saddle at infinity joins the stable manifold of the other saddle at infinity. These results explain prior reports for one of the models...

The Glycolytic Metabolite, Fructose-1,6-bisphosphate, Blocks Epileptiform Bursts by Attenuating Voltage-Activated Calcium Currents in Hippocampal Slices

Directory of Open Access Journals (Sweden)

Li-Rong Shao

2018-06-01

Full Text Available Manipulation of metabolic pathways (e.g., ketogenic diet (KD, glycolytic inhibition alters neural excitability and represents a novel strategy for treatment of drug-refractory seizures. We have previously shown that inhibition of glycolysis suppresses epileptiform activity in hippocampal slices. In the present study, we aimed to examine the role of a “branching” metabolic pathway stemming off glycolysis (i.e., the pentose-phosphate pathway, PPP in regulating seizure activity, by using a potent PPP stimulator and glycolytic intermediate, fructose-1,6-bisphosphate (F1,6BP. Employing electrophysiological approaches, we investigated the action of F1,6BP on epileptiform population bursts, intrinsic neuronal firing, glutamatergic and GABAergic synaptic transmission and voltage-activated calcium currents (ICa in the CA3 area of hippocampal slices. Bath application of F1,6BP (2.5–5 mM blocked epileptiform population bursts induced in Mg2+-free medium containing 4-aminopyridine, in ~2/3 of the slices. The blockade occurred relatively rapidly (~4 min, suggesting an extracellular mechanism. However, F1,6BP did not block spontaneous intrinsic firing of the CA3 neurons (when synaptic transmission was eliminated with DNQX, AP-5 and SR95531, nor did it significantly reduce AMPA or NMDA receptor-mediated excitatory postsynaptic currents (EPSCAMPA and EPSCNMDA. In contrast, F1,6BP caused moderate reduction (~50% in GABAA receptor-mediated current, suggesting it affects excitatory and inhibitory synapses differently. Finally and unexpectedly, F1,6BP consistently attenuated ICa by ~40% without altering channel activation or inactivation kinetics, which may explain its anticonvulsant action, at least in this in vitro seizure model. Consistent with these results, epileptiform population bursts in CA3 were readily blocked by the nonspecific Ca2+ channel blocker, CdCl2 (20 μM, suggesting that these bursts are calcium dependent. Altogether, these data

Tributyltin-induced apoptosis requires glycolytic adenosine trisphosphate production.

Science.gov (United States)

Stridh, H; Fava, E; Single, B; Nicotera, P; Orrenius, S; Leist, M

1999-10-01

The toxicity of tributyltin chloride (TBT) involves Ca(2+) overload, cytoskeletal damage, and mitochondrial failure leading to cell death by apoptosis or necrosis. Here, we examined whether the intracellular ATP level modulates the mode of cell death after exposure to TBT. When Jurkat cells were energized by the mitochondrial substrate, pyruvate, low concentrations of TBT (1-2 microM) triggered an immediate depletion of intracellular ATP followed by necrotic death. When ATP levels were maintained by the addition of glucose, the mode of cell death was typically apoptotic. Glycolytic ATP production was required for apoptosis at two distinct steps. First, maintenance of adequate ATP levels accelerated the decrease of mitochondrial membrane potential, and the release of the intermembrane proteins adenylate kinase and cytochrome c from mitochondria. A possible role of the adenine nucleotide exchanger in this first ATP-dependent step is suggested by experiments performed with the specific inhibitor, bongkrekic acid. This substance delayed cytochrome c release in a manner similar to that caused by ATP depletion. Second, caspase activation following cytochrome c release was only observed in ATP-containing cells. Bcl-2 had only a minor effect on TBT-triggered caspase activation or cell death. We conclude that intracellular ATP concentrations control the mode of cell death in TBT-treated Jurkat cells at both the mitochondrial and caspase activation levels.

Hydrogen production and metabolic flux analysis of metabolically engineered Escherichia coli strains

Energy Technology Data Exchange (ETDEWEB)

Kim, Seohyoung; Seol, Eunhee; Park, Sunghoon [Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735 (Korea); Oh, You-Kwan [Bioenergy Research Center, Korea Institute of Energy Research, Daejeon 305-543 (Korea); Wang, G.Y. [Department of Oceanography, University of Hawaii at Manoa Honolulu, HI 96822 (United States)

2009-09-15

Escherichia coli can produce H{sub 2} from glucose via formate hydrogen lyase (FHL). In order to improve the H{sub 2} production rate and yield, metabolically engineered E. coli strains, which included pathway alterations in their H{sub 2} production and central carbon metabolism, were developed and characterized by batch experiments and metabolic flux analysis. Deletion of hycA, a negative regulator for FHL, resulted in twofold increase of FHL activity. Deletion of two uptake hydrogenases (1 (hya) and hydrogenase 2 (hyb)) increased H{sub 2} production yield from 1.20 mol/mol glucose to 1.48 mol/mol glucose. Deletion of lactate dehydrogenase (ldhA) and fumarate reductase (frdAB) further improved the H{sub 2} yield; 1.80 mol/mol glucose under high H{sub 2} pressure or 2.11 mol/mol glucose under reduced H{sub 2} pressure. Several batch experiments at varying concentrations of glucose (2.5-10 g/L) and yeast extract (0.3 or 3.0 g/L) were conducted for the strain containing all these genetic alternations, and their carbon and energy balances were analyzed. The metabolic flux analysis revealed that deletion of ldhA and frdAB directed most of the carbons from glucose to the glycolytic pathway leading to H{sub 2} production by FHL, not to the pentose phosphate pathway. (author)

Augmentation of glycolytic metabolism by meclizine is indispensable for protection of dorsal root ganglion neurons from hypoxia-induced mitochondrial compromise.

Science.gov (United States)

Zhuo, Ming; Gorgun, Murat F; Englander, Ella W

2016-10-01

To meet energy demands, dorsal root ganglion (DRG) neurons harbor high mitochondrial content, which renders them acutely vulnerable to disruptions of energy homeostasis. While neurons typically rely on mitochondrial energy production and have not been associated with metabolic plasticity, new studies reveal that meclizine, a drug, recently linked to modulations of energy metabolism, protects neurons from insults that disrupt energy homeostasis. We show that meclizine rapidly enhances glycolysis in DRG neurons and that glycolytic metabolism is indispensable for meclizine-exerted protection of DRG neurons from hypoxic stress. We report that supplementation of meclizine during hypoxic exposure prevents ATP depletion, preserves NADPH and glutathione stores, curbs reactive oxygen species (ROS) and attenuates mitochondrial clustering in DRG neurites. Using extracellular flux analyzer, we show that in cultured DRG neurons meclizine mitigates hypoxia-induced loss of mitochondrial respiratory capacity. Respiratory capacity is a measure of mitochondrial fitness and cell ability to meet fluctuating energy demands and therefore, a key determinant of cellular fate. While meclizine is an 'old' drug with long record of clinical use, its ability to modulate energy metabolism has been uncovered only recently. Our findings documenting neuroprotection by meclizine in a setting of hypoxic stress reveal previously unappreciated metabolic plasticity of DRG neurons as well as potential for pharmacological harnessing of the newly discovered metabolic plasticity for protection of peripheral nervous system under mitochondria compromising conditions. Copyright © 2016 Elsevier Inc. All rights reserved.

From COS ecosystem fluxes to GPP: integrating soil, branch and ecosystem fluxes.

Science.gov (United States)

Kooijmans, L.; Maseyk, K. S.; Vesala, T.; Mammarella, I.; Baker, I. T.; Seibt, U.; Sun, W.; Aalto, J.; Franchin, A.; Kolari, P.; Keskinen, H.; Levula, J.; Chen, H.

2016-12-01

The close coupling of Carbonyl Sulfide (COS) and CO2 due to a similar uptake pathway into plant stomata makes COS a promising new tracer that can potentially be used to partition the Net Ecosystem Exchange into gross primary production (GPP) and respiration. Although ecosystem-scale measurements have been made at several sites, the contribution of different ecosystem components to the total COS budget is often unknown. Besides that, the average Leaf Relative Uptake (LRU) ratio needs to be better determined to accurately translate COS ecosystem fluxes into GPP estimates when the simple linear correlation between GPP estimates and COS plant uptake is used. We performed two campaigns in the summer of 2015 and 2016 at the SMEAR II site in Hyytiälä, Finland to provide better constrained COS flux data for boreal forests. A combination of COS measurements were made during both years, i.e. atmospheric profile concentrations up to 125 m, eddy-covariance fluxes and soil chamber fluxes. In addition to these, branch chamber measurements were done in 2016 in an attempt to observe the LRU throughout the whole season. The LRU ratio shows an exponential correlation with photosynthetic active radiation (PAR) but is constant for PAR levels above 500 µmol m-2 s-1. Mid-day LRU values are 1.0 (aspen) and 1.5 (pine). The correlation between LRU and PAR can be explained by the fact that COS is hydrolyzed with the presence of the enzyme carbonic anhydrase, and is not light dependent, whereas the photosynthetic uptake of CO2 is. We observed nighttime fluxes on the order of 25-30 % of the daily maximum COS uptake. Soils are a small sink of COS and contribute to 3 % of the total ecosystem COS flux during daytime. In a comparison between observed and simulated fluxes from the Simple Biosphere (SiB) model, the modelled COS and CO2 ecosystem fluxes are on average 40 % smaller than the observed fluxes, however, the Ecosystem Relative Uptake (ERU) ratios are identical at a value of 1.9 ± 0

Measurements of EUV coronal holes and open magnetic flux

International Nuclear Information System (INIS)

Lowder, C.; Qiu, J.; Leamon, R.; Liu, Y.

2014-01-01

Coronal holes are regions on the Sun's surface that map the footprints of open magnetic field lines. We have developed an automated routine to detect and track boundaries of long-lived coronal holes using full-disk extreme-ultraviolet (EUV) images obtained by SOHO/EIT, SDO/AIA, and STEREO/EUVI. We measure coronal hole areas and magnetic flux in these holes, and compare the measurements with calculations by the potential field source surface (PFSS) model. It is shown that, from 1996 through 2010, the total area of coronal holes measured with EIT images varies between 5% and 17% of the total solar surface area, and the total unsigned open flux varies between (2-5)× 10 22 Mx. The solar cycle dependence of these measurements is similar to the PFSS results, but the model yields larger hole areas and greater open flux than observed by EIT. The AIA/EUVI measurements from 2010-2013 show coronal hole area coverage of 5%-10% of the total surface area, with significant contribution from low latitudes, which is under-represented by EIT. AIA/EUVI have measured much enhanced open magnetic flux in the range of (2-4)× 10 22 Mx, which is about twice the flux measured by EIT, and matches with the PFSS calculated open flux, with discrepancies in the location and strength of coronal holes. A detailed comparison between the three measurements (by EIT, AIA-EUVI, and PFSS) indicates that coronal holes in low latitudes contribute significantly to the total open magnetic flux. These low-latitude coronal holes are not well measured with either the He I 10830 line in previous studies, or EIT EUV images; neither are they well captured by the static PFSS model. The enhanced observations from AIA/EUVI allow a more accurate measure of these low-latitude coronal holes and their contribution to open magnetic flux.

Expression of genes encoding F-1-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis

DEFF Research Database (Denmark)

Købmann, Brian Jensen; Solem, Christian; Pedersen, M.B.

2002-01-01

of the genes encoding F-1-ATPase was found to decrease the intracellular energy level and resulted in a decrease in the growth rate. The yield of biomass also decreased, which showed that the incorporated F-1-ATPase activity caused glycolysis to be uncoupled from biomass production. The increase in ATPase...... threefold in nongrowing cells resuspended in buffer, but in steadily growing cells no increase in flux was observed. The latter result shows that glycolysis occurs close to its maximal capacity and indicates that control of the glycolytic flux under these conditions resides in the glycolytic reactions...

Quantitative method for measuring heat flux emitted from a cryogenic object

Science.gov (United States)

Duncan, R.V.

1993-03-16

The present invention is a quantitative method for measuring the total heat flux, and of deriving the total power dissipation, of a heat-fluxing object which includes the steps of placing an electrical noise-emitting heat-fluxing object in a liquid helium bath and measuring the superfluid transition temperature of the bath. The temperature of the liquid helium bath is thereafter reduced until some measurable parameter, such as the electrical noise, exhibited by the heat-fluxing object or a temperature-dependent resistive thin film in intimate contact with the heat-fluxing object, becomes greatly reduced. The temperature of the liquid helum bath is measured at this point. The difference between the superfluid transition temperature of the liquid helium bath surrounding the heat-fluxing object, and the temperature of the liquid helium bath when the electrical noise emitted by the heat-fluxing object becomes greatly reduced, is determined. The total heat flux from the heat-fluxing object is determined as a function of this difference between these temperatures. In certain applications, the technique can be used to optimize thermal design parameters of cryogenic electronics, for example, Josephson junction and infrared sensing devices.

Quantitative method for measuring heat flux emitted from a cryogenic object

International Nuclear Information System (INIS)

Duncan, R.V.

1993-01-01

The present invention is a quantitative method for measuring the total heat flux, and of deriving the total power dissipation, of a heat-fluxing object which includes the steps of placing an electrical noise-emitting heat-fluxing object in a liquid helium bath and measuring the superfluid transition temperature of the bath. The temperature of the liquid helium bath is thereafter reduced until some measurable parameter, such as the electrical noise, exhibited by the heat-fluxing object or a temperature-dependent resistive thin film in intimate contact with the heat-fluxing object, becomes greatly reduced. The temperature of the liquid helum bath is measured at this point. The difference between the superfluid transition temperature of the liquid helium bath surrounding the heat-fluxing object, and the temperature of the liquid helium bath when the electrical noise emitted by the heat-fluxing object becomes greatly reduced, is determined. The total heat flux from the heat-fluxing object is determined as a function of this difference between these temperatures. In certain applications, the technique can be used to optimize thermal design parameters of cryogenic electronics, for example, Josephson junction and infrared sensing devices

Monitoring of glycolytic activity secondary to ischaemia in knee replacement surgery.

Science.gov (United States)

León-Muñoz, V J; Lisón-Almagro, A J; Hernández-García, C H; López-López, M

2018-04-17

To non-invasively assess tissue lesion secondary to ischaemia applied during knee replacement surgery. Secondary objectives: to assess whether this lesion correlates with the duration of ischaemia and whether instrumental and gender variables influence it. Prospective cohort study. Pre and postoperative serum lactate levels have been determined as an indicator of glycolytic activity secondary to ischaemia in 88 patients. Serum lactate determination was performed by reactive strips of enzymatic-amperometric detection on capillary blood. Preoperative serum lactate levels (mean and SD): 2.467±1.036 mmol/L. Postoperative serum lactate levels: 3.938±2.018 mmol/L. Ischaemia time 102.98±18.25minutes. Postoperative serum lactate levels were significantly higher than preoperative lactate levels. There are no statistical differences according to the time that the ischaemia was prolonged, gender or type of instrumentation used. In our study, postoperative serum lactate values were significantly higher than preoperative lactate values, with no correlation to the duration of ischaemia during knee replacement surgery. Copyright © 2018 SECOT. Publicado por Elsevier España, S.L.U. All rights reserved.

Effect of aspirin and prostaglandins on the carbohydrate metabolism in albino rats.: glucose oxidation through different pathways and glycolytic enzymes

International Nuclear Information System (INIS)

Balasubramanian, A.; Ramakrishnan, S.

1980-01-01

The effect of chronic and acute doses of aspirin and prostaglandins F2α and E2 individually on the oxidation of glucose through Embden Meyerhof-TCA cycle and pentose phosphate pathways and some key glycolytic enzymes of liver were studied in male albino rats. Studies were extended to find the combined effect of PGF2α and E2 with an acute dose of aspirin. There was increased utilisation of both 1- 14 C glucose and 6- 14 C glucose on aspirin treatment. However, the metabolism through the EM-TCA pathway was more pronounced as shown by a reduced ratio of 14 CO 2 from 1- 14 C and 6- 14 C glucose. Two hepatic key glycolytic enzymes viz. hexokinase and pyruvate kinase were increased due to aspirin treatment. Withdrawal of aspirin corrected the above impaired carbohydrate metabolism in liver. Prostaglandin F2α also caused a reduction in the utilisation of 1- 14 C glucose, while PGE2 recorded an increase in the utilisation of both 1- 14 C and 6- 14 C glucose when compared to controls, indicating that different members of prostaglandins could affect metabolisms and differently. Administration of the PGs and aspirin together showed an increase in the utilisation of 6- 14 C glucose. (auth.)

Total mercury and methylmercury fluxes via emerging insects in recently flooded hydroelectric reservoirs and a natural lake

International Nuclear Information System (INIS)

Tremblay, Alain; Lucotte, Marc; Cloutier, Louise

1998-01-01

Total mercury (total Hg) concentrations in emerging aquatic insects ranged from 140 to 1500 ng g -1 dry wt. in two hydroelectric reservoirs of northern Quebec compared with 50-160 ng g -1 dry wt. in a natural lake. Methylmercury (MeHg) concentrations were somewhat lower, ranging from 35 to 800 ng Hg g -1 dry wt. in reservoirs and from 29 to 90 ng g -1 dry wt. in the natural lake. Contamination of insect taxa of reservoirs was on average 2-3 times higher than their counterparts in the natural lake. There was no difference between total Hg and MeHg concentrations of insects sampled from flooded forest soils and flooded peatland, although total Hg and MeHg concentrations differed between flooded peatland and flooded forest soils themselves. Insect biomasses were approx. two times higher in the reservoirs than in the natural lake (580-2200 mg m -2 year -1 dry wt., 950 mg m -2 year -1 dry wt., respectively); chironomids dominated in the reservoirs and trichopterans dominated in the natural lake. Similarly, total MeHg fluxes via emerging insects were approx. 2-4 times higher in reservoirs than that of the natural lake (55-224 ng MeHg m -2 year -1 dry wt., 74 ng MeHg m -2 year -1 dry wt., respectively). Our results show the importance of the insect community in the transfer of MeHg from flooded soils and flooded peatlands to fish, and that this pathway probably makes a significant contribution to the rapid rise of Hg levels in the fish community after flooding

Measurements of EUV coronal holes and open magnetic flux

Energy Technology Data Exchange (ETDEWEB)

Lowder, C.; Qiu, J.; Leamon, R. [Department of Physics, Montana State University, Bozeman, MT 59717 (United States); Liu, Y., E-mail: clowder@solar.physics.montana.edu [W. W. Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA 94305 (United States)

2014-03-10

Coronal holes are regions on the Sun's surface that map the footprints of open magnetic field lines. We have developed an automated routine to detect and track boundaries of long-lived coronal holes using full-disk extreme-ultraviolet (EUV) images obtained by SOHO/EIT, SDO/AIA, and STEREO/EUVI. We measure coronal hole areas and magnetic flux in these holes, and compare the measurements with calculations by the potential field source surface (PFSS) model. It is shown that, from 1996 through 2010, the total area of coronal holes measured with EIT images varies between 5% and 17% of the total solar surface area, and the total unsigned open flux varies between (2-5)× 10{sup 22} Mx. The solar cycle dependence of these measurements is similar to the PFSS results, but the model yields larger hole areas and greater open flux than observed by EIT. The AIA/EUVI measurements from 2010-2013 show coronal hole area coverage of 5%-10% of the total surface area, with significant contribution from low latitudes, which is under-represented by EIT. AIA/EUVI have measured much enhanced open magnetic flux in the range of (2-4)× 10{sup 22} Mx, which is about twice the flux measured by EIT, and matches with the PFSS calculated open flux, with discrepancies in the location and strength of coronal holes. A detailed comparison between the three measurements (by EIT, AIA-EUVI, and PFSS) indicates that coronal holes in low latitudes contribute significantly to the total open magnetic flux. These low-latitude coronal holes are not well measured with either the He I 10830 line in previous studies, or EIT EUV images; neither are they well captured by the static PFSS model. The enhanced observations from AIA/EUVI allow a more accurate measure of these low-latitude coronal holes and their contribution to open magnetic flux.

Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

LENUS (Irish Health Repository)

Burleigh, Susan C

2011-10-18

Abstract Background The glycosylation of recombinant proteins can be altered by a range of parameters including cellular metabolism, metabolic flux and the efficiency of the glycosylation process. We present an experimental set-up that allows determination of these key processes associated with the control of N-linked glycosylation of recombinant proteins. Results Chinese hamster ovary cells (CHO) were cultivated in shake flasks at 0 mM glutamine and displayed a reduced growth rate, glucose metabolism and a slower decrease in pH, when compared to other glutamine-supplemented cultures. The N-linked glycosylation of recombinant human chorionic gonadotrophin (HCG) was also altered under these conditions; the sialylation, fucosylation and antennarity decreased, while the proportion of neutral structures increased. A continuous culture set-up was subsequently used to understand the control of HCG glycosylation in the presence of varied glutamine concentrations; when glycolytic flux was reduced in the absence of glutamine, the glycosylation changes that were observed in shake flask culture were similarly detected. The intracellular content of UDP-GlcNAc was also reduced, which correlated with a decrease in sialylation and antennarity of the N-linked glycans attached to HCG. Conclusions The use of metabolic flux analysis illustrated a case of steady state multiplicity, where use of the same operating conditions at each steady state resulted in altered flux through glycolysis and the TCA cycle. This study clearly demonstrated that the control of glycoprotein microheterogeneity may be examined by use of a continuous culture system, metabolic flux analysis and assay of intracellular nucleotides. This system advances our knowledge of the relationship between metabolic flux and the glycosylation of biotherapeutics in CHO cells and will be of benefit to the bioprocessing industry.

A Petiveria alliacea standardized fraction induces breast adenocarcinoma cell death by modulating glycolytic metabolism.

Science.gov (United States)

Hernández, John Fredy; Urueña, Claudia Patricia; Cifuentes, Maria Claudia; Sandoval, Tito Alejandro; Pombo, Luis Miguel; Castañeda, Diana; Asea, Alexzander; Fiorentino, Susana

2014-05-14

Folk medicine uses aqueous and alcoholic extracts from Petiveria alliacea (Phytolaccaceae) in leukemia and breast cancer treatment in the Caribbean, Central and South America. Herein, we validated the biological activity of a Petiveria alliacea fraction using a metastatic breast adenocarcinoma model (4T1). Petiveria alliacea fraction biological activity was determined estimating cell proliferation, cell colony growth capacity and apoptosis (caspase-3 activity, DNA fragmentation and mitochondrial membrane potential) in 4T1 cells. Petiveria alliacea was used at IC₅₀ concentration (29 µg/mL) and 2 dilutions below, doxorubicin at 0.27 µg/mL (positive control) and dibenzyl disulfide at 2.93 µg/mL (IC50 fraction marker compound). Proteomic estimations were analyzed by LC-MS-MS. Protein level expression was confirmed by RT-PCR. Glucose and lactate levels were measured by enzymatic assays. LD50 was established in BALB/c mice and antitumoral activity evaluated in mice transplanted with GFP-tagged 4T1 cells. Mice were treated with Petiveria alliacea fraction via I.P (182 mg/kg corresponding to 1/8 of LD₅₀ and 2 dilutions below). Petiveria alliacea fraction in vitro induces 4T1 cells apoptosis, caspase-3 activation, DNA fragmentation without mitochondria membrane depolarization, and decreases cell colony growth capacity. Also, changes in glycolytic enzymes expression cause a decrease in glucose uptake and lactate production. Fraction also promotes breast primary tumor regression in BALB/c mice transplanted with GFP-tagged 4T1 cells. A fraction of Petiveria alliacea leaves and stems induces in vitro cell death and in vivo tumor regression in a murine breast cancer model. Our results validate in partly, the traditional use of Petiveria alliacea in breast cancer treatment, revealing a new way of envisioning Petiveria alliacea biological activity. The fraction effect on the glycolytic pathway enzymes contributes to explain the antiproliferative and antitumor activities

Fluxes of total reactive atmospheric nitrogen (ΣNr using eddy covariance above arable land

Directory of Open Access Journals (Sweden)

Christophe R. Flechard

2013-02-01

Full Text Available The amount and timing of reactive nitrogen exchange between agricultural land and the atmosphere play a key role in evaluating ecosystem productivity and in addressing atmospheric nitrogen budgets and transport. With the recent development of the Total Reactive Atmospheric Nitrogen Converter (TRANC apparatus, a methodology has been provided for continuous measurement of the sum of all airborne nitrogen containing species (ΣNr allowing for diurnal and seasonal investigations. We present ΣNr concentration and net flux data from an 11-month field campaign conducted at an arable field using the TRANC system within an eddy-covariance setup. Clear diurnal patterns of both ΣNr concentrations and fluxes with significant dependencies on atmospheric stability and stomatal regulation were observed in the growing season. TRANC data were compared with monthly-averaged concentrations and dry deposition rates of selected Nr compounds using DELTA denuders and ensemble-averages of four inferential models, respectively. Similar seasonal trends were found for Nr concentrations from DELTA and TRANC measurements with values from the latter being considerably higher than those of DELTA denuders. The variability of the difference between these two systems could be explained by seasonally changing source locations of NOx contributions to the TRANC signal. As soil and vegetation Nr emissions to the atmosphere are generally not treated by inferential (dry deposition models, TRANC data showed lower monthly deposition rates than those obtained from inferential modelling. Net ΣNr exchange was almost neutral (~0.072 kg N ha−1 at the end of the observation period. However, during most parts of the year, slight but permanent net ΣNr deposition was found. Our measurements demonstrate that fertilizer addition followed by substantial ΣNr emissions plays a crucial role in a site's annual atmospheric nitrogen budget. As long-term Nr measurements with high temporal

Comparative effect of lidocaine and bupivacaine on glucose uptake and lactate production in the perfused working rat heart

International Nuclear Information System (INIS)

Cronau, L.H. Jr.; Merin, R.G.; Aboulish, E.; Steenberg, M.L.; Maljorda, A.

1986-01-01

It has been suggested that at equivalent therapeutic concentrations, lidocaine and bupivacaine may have different cardiotoxic potency. In the isolated working rat heart preparation, the effect of a range of lidocaine and bupivacaine concentrations on glucose uptake and lactate production (LP) were observed. Insulin was added, 10 μ/L, to Ringer's solution containing 3 H-labeled glucose to measure the glycolytic flux (GF). The effect of the local anesthetics on LP at the indicated concentrations were similar. Lidocaine appears to depress the glycolytic flux from exogenous glucose to a lesser degree. Bupivacaine, 10 mg/L, depresses VO 2 to a greater degree than does lidocaine, 40 mg/L

KoFlux: Korean Regional Flux Network in AsiaFlux

Science.gov (United States)

Kim, J.

2002-12-01

AsiaFlux, the Asian arm of FLUXNET, held the Second International Workshop on Advanced Flux Network and Flux Evaluation in Jeju Island, Korea on 9-11 January 2002. In order to facilitate comprehensive Asia-wide studies of ecosystem fluxes, the meeting launched KoFlux, a new Korean regional network of long-term micrometeorological flux sites. For a successful assessment of carbon exchange between terrestrial ecosystems and the atmosphere, an accurate measurement of surface fluxes of energy and water is one of the prerequisites. During the 7th Global Energy and Water Cycle Experiment (GEWEX) Asian Monsoon Experiment (GAME) held in Nagoya, Japan on 1-2 October 2001, the Implementation Committee of the Coordinated Enhanced Observing Period (CEOP) was established. One of the immediate tasks of CEOP was and is to identify the reference sites to monitor energy and water fluxes over the Asian continent. Subsequently, to advance the regional and global network of these reference sites in the context of both FLUXNET and CEOP, the Korean flux community has re-organized the available resources to establish a new regional network, KoFlux. We have built up domestic network sites (equipped with wind profiler and radiosonde measurements) over deciduous and coniferous forests, urban and rural rice paddies and coastal farmland. As an outreach through collaborations with research groups in Japan, China and Thailand, we also proposed international flux sites at ecologically and climatologically important locations such as a prairie on the Tibetan plateau, tropical forest with mixed and rapid land use change in northern Thailand. Several sites in KoFlux already begun to accumulate interesting data and some highlights are presented at the meeting. The sciences generated by flux networks in other continents have proven the worthiness of a global array of micrometeorological flux towers. It is our intent that the launch of KoFlux would encourage other scientists to initiate and

Evidence for brain glucose dysregulation in Alzheimer's disease.

Science.gov (United States)

An, Yang; Varma, Vijay R; Varma, Sudhir; Casanova, Ramon; Dammer, Eric; Pletnikova, Olga; Chia, Chee W; Egan, Josephine M; Ferrucci, Luigi; Troncoso, Juan; Levey, Allan I; Lah, James; Seyfried, Nicholas T; Legido-Quigley, Cristina; O'Brien, Richard; Thambisetty, Madhav

2018-03-01

It is unclear whether abnormalities in brain glucose homeostasis are associated with Alzheimer's disease (AD) pathogenesis. Within the autopsy cohort of the Baltimore Longitudinal Study of Aging, we measured brain glucose concentration and assessed the ratios of the glycolytic amino acids, serine, glycine, and alanine to glucose. We also quantified protein levels of the neuronal (GLUT3) and astrocytic (GLUT1) glucose transporters. Finally, we assessed the relationships between plasma glucose measured before death and brain tissue glucose. Higher brain tissue glucose concentration, reduced glycolytic flux, and lower GLUT3 are related to severity of AD pathology and the expression of AD symptoms. Longitudinal increases in fasting plasma glucose levels are associated with higher brain tissue glucose concentrations. Impaired glucose metabolism due to reduced glycolytic flux may be intrinsic to AD pathogenesis. Abnormalities in brain glucose homeostasis may begin several years before the onset of clinical symptoms. Copyright © 2017 the Alzheimer's Association. All rights reserved.

Evaluation of NASA's Carbon Monitoring System (CMS) Flux Pilot: Terrestrial CO2 Fluxes

Science.gov (United States)

Fisher, J. B.; Polhamus, A.; Bowman, K. W.; Collatz, G. J.; Potter, C. S.; Lee, M.; Liu, J.; Jung, M.; Reichstein, M.

2011-12-01

NASA's Carbon Monitoring System (CMS) flux pilot project combines NASA's Earth System models in land, ocean and atmosphere to track surface CO2 fluxes. The system is constrained by atmospheric measurements of XCO2 from the Japanese GOSAT satellite, giving a "big picture" view of total CO2 in Earth's atmosphere. Combining two land models (CASA-Ames and CASA-GFED), two ocean models (ECCO2 and NOBM) and two atmospheric chemistry and inversion models (GEOS-5 and GEOS-Chem), the system brings together the stand-alone component models of the Earth System, all of which are run diagnostically constrained by a multitude of other remotely sensed data. Here, we evaluate the biospheric land surface CO2 fluxes (i.e., net ecosystem exchange, NEE) as estimated from the atmospheric flux inversion. We compare against the prior bottom-up estimates (e.g., the CASA models) as well. Our evaluation dataset is the independently derived global wall-to-wall MPI-BGC product, which uses a machine learning algorithm and model tree ensemble to "scale-up" a network of in situ CO2 flux measurements from 253 globally-distributed sites in the FLUXNET network. The measurements are based on the eddy covariance method, which uses observations of co-varying fluxes of CO2 (and water and energy) from instruments on towers extending above ecosystem canopies; the towers integrate fluxes over large spatial areas (~1 km2). We present global maps of CO2 fluxes and differences between products, summaries of fluxes by TRANSCOM region, country, latitude, and biome type, and assess the time series, including timing of minimum and maximum fluxes. This evaluation shows both where the CMS is performing well, and where improvements should be directed in further work.

HSulf-1 deficiency dictates a metabolic reprograming of glycolysis and TCA cycle in ovarian cancer.

Science.gov (United States)

Mondal, Susmita; Roy, Debarshi; Camacho-Pereira, Juliana; Khurana, Ashwani; Chini, Eduardo; Yang, Lifeng; Baddour, Joelle; Stilles, Katherine; Padmabandu, Seth; Leung, Sam; Kalloger, Steve; Gilks, Blake; Lowe, Val; Dierks, Thomas; Hammond, Edward; Dredge, Keith; Nagrath, Deepak; Shridhar, Viji

2015-10-20

Warburg effect has emerged as a potential hallmark of many cancers. However, the molecular mechanisms that led to this metabolic state of aerobic glycolysis, particularly in ovarian cancer (OVCA) have not been completely elucidated. HSulf-1 predominantly functions by limiting the bioavailability of heparan binding growth factors and hence their downstream signaling. Here we report that HSulf-1, a known putative tumor suppressor, is a negative regulator of glycolysis. Silencing of HSulf-1 expression in OV202 cell line increased glucose uptake and lactate production by upregulating glycolytic genes such as Glut1, HKII, LDHA, as well as metabolites. Conversely, HSulf-1 overexpression in TOV21G cells resulted in the down regulation of glycolytic enzymes and reduced glycolytic phenotype, supporting the role of HSulf-1 loss in enhanced aerobic glycolysis. HSulf-1 deficiency mediated glycolytic enhancement also resulted in increased inhibitory phosphorylation of pyruvate dehydrogenase (PDH) thus blocking the entry of glucose flux into TCA cycle. Consistent with this, metabolomic and isotope tracer analysis showed reduced glucose flux into TCA cycle. Moreover, HSulf-1 loss is associated with lower oxygen consumption rate (OCR) and impaired mitochondrial function. Mechanistically, lack of HSulf-1 promotes c-Myc induction through HB-EGF-mediated p-ERK activation. Pharmacological inhibition of c-Myc reduced HB-EGF induced glycolytic enzymes implicating a major role of c-Myc in loss of HSulf-1 mediated altered glycolytic pathway in OVCA. Similarly, PG545 treatment, an agent that binds to heparan binding growth factors and sequesters growth factors away from their ligand also blocked HB-EGF signaling and reduced glucose uptake in vivo in HSulf-1 deficient cells.

Shigella reroutes host cell central metabolism to obtain high-flux nutrient supply for vigorous intracellular growth.

Science.gov (United States)

Kentner, David; Martano, Giuseppe; Callon, Morgane; Chiquet, Petra; Brodmann, Maj; Burton, Olga; Wahlander, Asa; Nanni, Paolo; Delmotte, Nathanaël; Grossmann, Jonas; Limenitakis, Julien; Schlapbach, Ralph; Kiefer, Patrick; Vorholt, Julia A; Hiller, Sebastian; Bumann, Dirk

2014-07-08

Shigella flexneri proliferate in infected human epithelial cells at exceptionally high rates. This vigorous growth has important consequences for rapid progression to life-threatening bloody diarrhea, but the underlying metabolic mechanisms remain poorly understood. Here, we used metabolomics, proteomics, and genetic experiments to determine host and Shigella metabolism during infection in a cell culture model. The data suggest that infected host cells maintain largely normal fluxes through glycolytic pathways, but the entire output of these pathways is captured by Shigella, most likely in the form of pyruvate. This striking strategy provides Shigella with an abundant favorable energy source, while preserving host cell ATP generation, energy charge maintenance, and survival, despite ongoing vigorous exploitation. Shigella uses a simple three-step pathway to metabolize pyruvate at high rates with acetate as an excreted waste product. The crucial role of this pathway for Shigella intracellular growth suggests targets for antimicrobial chemotherapy of this devastating disease.

Chain Rule Approach for Calculating the Time-Derivative of Flux

Energy Technology Data Exchange (ETDEWEB)

Langenbrunner, James R. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Booker, Jane M. [Booker Scientific, Fredericksburg, TX (United States)

2017-10-03

The reaction history (gamma-flux observable) is mathematically studied by using the chain rule for taking the total-time derivatives. That is, the total time-derivative of flux is written as the product of the ion temperature derivative with respect to time and the derivative of the flux with respect to ion temperature. Some equations are derived using the further simplification that the fusion reactivity is a parametrized function of ion temperature, T. Deuterium-tritium (D-T) fusion is used as the application with reactivity calculations from three established reactivity parametrizations.

Specification of ROP flux shape

Energy Technology Data Exchange (ETDEWEB)

Min, Byung Joo [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of); Gray, A [Atomic Energy of Canada Ltd., Chalk River, ON (Canada)

1997-06-01

The CANDU 9 480/SEU core uses 0.9% SEU (Slightly Enriched Uranium) fuel. The use f SEU fuel enables the reactor to increase the radial power form factor from 0.865, which is typical in current natural uranium CANDU reactors, to 0.97 in the nominal CANDU 9 480/SEU core. The difference is a 12% increase in reactor power. An additional 5% increase can be achieved due to a reduced refuelling ripple. The channel power limits were also increased by 3% for a total reactor power increase of 20%. This report describes the calculation of neutron flux distributions in the CANDU 9 480/SEU core under conditions specified by the C and I engineers. The RFSP code was used to calculate of neutron flux shapes for ROP analysis. Detailed flux values at numerous potential detector sites were calculated for each flux shape. (author). 6 tabs., 70 figs., 4 refs.

Specification of ROP flux shape

International Nuclear Information System (INIS)

Min, Byung Joo; Gray, A.

1997-06-01

The CANDU 9 480/SEU core uses 0.9% SEU (Slightly Enriched Uranium) fuel. The use f SEU fuel enables the reactor to increase the radial power form factor from 0.865, which is typical in current natural uranium CANDU reactors, to 0.97 in the nominal CANDU 9 480/SEU core. The difference is a 12% increase in reactor power. An additional 5% increase can be achieved due to a reduced refuelling ripple. The channel power limits were also increased by 3% for a total reactor power increase of 20%. This report describes the calculation of neutron flux distributions in the CANDU 9 480/SEU core under conditions specified by the C and I engineers. The RFSP code was used to calculate of neutron flux shapes for ROP analysis. Detailed flux values at numerous potential detector sites were calculated for each flux shape. (author). 6 tabs., 70 figs., 4 refs

Metabolic fluxes in the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis, two members of the marine Roseobacter clade

Directory of Open Access Journals (Sweden)

Rabus Ralf

2009-09-01

Full Text Available Abstract Background In the present work the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis was studied at the level of metabolic fluxes. These two strains belong to the marine Roseobacter clade, a dominant bacterial group in various marine habitats, and represent surface-associated, biofilm-forming growth (P. gallaeciensis and symbiotic growth with eukaryotic algae (D. shibae. Based on information from recently sequenced genomes, a rich repertoire of pathways has been identified in the carbon core metabolism of these organisms, but little is known about the actual contribution of the various reactions in vivo. Results Using 13C labelling techniques in specifically designed experiments, it could be shown that glucose-grown cells of D. shibae catabolise the carbon source exclusively via the Entner-Doudoroff pathway, whereas alternative routes of glycolysis and the pentose phosphate pathway are obviously utilised for anabolic purposes only. Enzyme assays confirmed this flux pattern and link the lack of glycolytic flux to the absence of phosphofructokinase activity. The previously suggested formation of phosphoenolpyruvate from pyruvate during mixotrophic CO2 assimilation was found to be inactive under the conditions studied. Moreover, it could be shown that pyruvate carboxylase is involved in CO2 assimilation and that the cyclic respiratory mode of the TCA cycle is utilised. Interestingly, the use of intracellular pathways was highly similar for P. gallaeciensis. Conclusion The present study reveals the first insight into pathway utilisation within the Roseobacter group. Fluxes through major intracellular pathways of the central carbon metabolism, which are closely linked to the various important traits found for the Roseobacter clade, could be determined. The close similarity of fluxes between the two physiologically rather different species might provide the first indication of more general key properties among

Course of the anaerobic alcohol fermentation by Saccharomyces cerevisiae simulated through a mathematical model of the glycolysis

Energy Technology Data Exchange (ETDEWEB)

Martiny, S C

1972-01-01

The model presented here attempts to simulate the course of the conversion of glucose to alcohol through a simulation of the glycolytic flux rate. The model is based on dynamic stationarity through the glycolytic reactions, equilibrium in the action of ATPase. The model does not simulate experimental data, mainly because ATPase cannot keep pace with ATP formation. The simulations stress the need for better understanding of the mechanism of ATP removal within the cell.

Monitoring to assess progress toward meeting the Assabet River, Massachusetts, phosphorus total maximum daily load - Aquatic macrophyte biomass and sediment-phosphorus flux

Science.gov (United States)

Zimmerman, Marc J.; Qian, Yu; Yong Q., Tian

2011-01-01

In 2004, the Total Maximum Daily Load (TMDL) for Total Phosphorus in the Assabet River, Massachusetts, was approved by the U.S. Environmental Protection Agency. The goal of the TMDL was to decrease the concentrations of the nutrient phosphorus to mitigate some of the instream ecological effects of eutrophication on the river; these effects were, for the most part, direct consequences of the excessive growth of aquatic macrophytes. The primary instrument effecting lower concentrations of phosphorus was to be strict control of phosphorus releases from four major wastewatertreatment plants in Westborough, Marlborough, Hudson, and Maynard, Massachusetts. The improvements to be achieved from implementing this control were lower concentrations of total and dissolved phosphorus in the river, a 50-percent reduction in aquatic-plant biomass, a 30-percent reduction in episodes of dissolved oxygen supersaturation, no low-flow dissolved oxygen concentrations less than 5.0 milligrams per liter, and a 90-percent reduction in sediment releases of phosphorus to the overlying water. In 2007, the U.S. Geological Survey, in cooperation with the Massachusetts Department of Environmental Protection, initiated studies to evaluate conditions in the Assabet River prior to the upgrading of wastewater-treatment plants to remove more phosphorus from their effluents. The studies, completed in 2008, implemented a visual monitoring plan to evaluate the extent and biomass of the floating macrophyte Lemna minor (commonly known as lesser duckweed) in five impoundments and evaluated the potential for phosphorus flux from sediments in impounded and free-flowing reaches of the river. Hydrologically, the two study years 2007 and 2008 were quite different. In 2007, summer streamflows, although low, were higher than average, and in 2008, the flows were generally higher than in 2007. Visually, the effects of these streamflow differences on the distribution of Lemna were obvious. In 2007, large amounts of

Critical flux determination by flux-stepping

DEFF Research Database (Denmark)

Beier, Søren; Jonsson, Gunnar Eigil

2010-01-01

In membrane filtration related scientific literature, often step-by-step determined critical fluxes are reported. Using a dynamic microfiltration device, it is shown that critical fluxes determined from two different flux-stepping methods are dependent upon operational parameters such as step...... length, step height, and.flux start level. Filtrating 8 kg/m(3) yeast cell suspensions by a vibrating 0.45 x 10(-6) m pore size microfiltration hollow fiber module, critical fluxes from 5.6 x 10(-6) to 1.2 x 10(-5) m/s have been measured using various step lengths from 300 to 1200 seconds. Thus......, such values are more or less useless in itself as critical flux predictors, and constant flux verification experiments have to be conducted to check if the determined critical fluxes call predict sustainable flux regimes. However, it is shown that using the step-by-step predicted critical fluxes as start...

Comparative effect of lidocaine and bupivacaine on glucose uptake and lactate production in the perfused working rat heart

Energy Technology Data Exchange (ETDEWEB)

Cronau, L.H. Jr.; Merin, R.G.; Aboulish, E.; Steenberg, M.L.; Maljorda, A.

1986-03-01

It has been suggested that at equivalent therapeutic concentrations, lidocaine and bupivacaine may have different cardiotoxic potency. In the isolated working rat heart preparation, the effect of a range of lidocaine and bupivacaine concentrations on glucose uptake and lactate production (LP) were observed. Insulin was added, 10 ..mu../L, to Ringer's solution containing /sup 3/H-labeled glucose to measure the glycolytic flux (GF). The effect of the local anesthetics on LP at the indicated concentrations were similar. Lidocaine appears to depress the glycolytic flux from exogenous glucose to a lesser degree. Bupivacaine, 10 mg/L, depresses VO/sub 2/ to a greater degree than does lidocaine, 40 mg/L.

Metabolic flux rearrangement in the amino acid metabolism reduces ammonia stress in the α1-antitrypsin producing human AGE1.HN cell line.

Science.gov (United States)

Priesnitz, Christian; Niklas, Jens; Rose, Thomas; Sandig, Volker; Heinzle, Elmar

2012-03-01

This study focused on metabolic changes in the neuronal human cell line AGE1.HN upon increased ammonia stress. Batch cultivations of α(1)-antitrypsin (A1AT) producing AGE1.HN cells were carried out in media with initial ammonia concentrations ranging from 0mM to 5mM. Growth, A1AT production, metabolite dynamics and finally metabolic fluxes calculated by metabolite balancing were compared. Growth and A1AT production decreased with increasing ammonia concentration. The maximum A1AT concentration decreased from 0.63g/l to 0.51g/l. Central energy metabolism remained relatively unaffected exhibiting only slightly increased glycolytic flux at high initial ammonia concentration in the medium. However, the amino acid metabolism was significantly changed. Fluxes through transaminases involved in amino acid degradation were reduced concurrently with a reduced uptake of amino acids. On the other hand fluxes through transaminases working in the direction of amino acid synthesis, i.e., alanine and phosphoserine, were increased leading to increased storage of excess nitrogen in extracellular alanine and serine. Glutamate dehydrogenase flux was reversed increasingly fixing free ammonia with increasing ammonia concentration. Urea production additionally observed was associated with arginine uptake by the cells and did not increase at high ammonia stress. It was therefore not used as nitrogen sink to remove excess ammonia. The results indicate that the AGE1.HN cell line can adapt to ammonia concentrations usually present during the cultivation process to a large extent by changing metabolism but with slightly reduced A1AT production and growth. Copyright © 2012 Elsevier Inc. All rights reserved.

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Science.gov (United States)

2012-01-01

Background The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as sole carbon source have been shown to improve product productivities and alleviate metabolic burden derived from protein production. Nevertheless, systematic quantitative studies on the relationships between the central metabolism and recombinant protein production in P. pastoris are still rather limited, particularly when growing this yeast on mixed carbon sources, thus hampering future metabolic network engineering strategies for improved protein production. Results The metabolic flux distribution in the central metabolism of P. pastoris growing on a mixed feed of glucose and methanol was analyzed by Metabolic Flux Analysis (MFA) using 13C-NMR-derived constraints. For this purpose, we defined new flux ratios for methanol assimilation pathways in P. pastoris cells growing on glucose:methanol mixtures. By using this experimental approach, the metabolic burden caused by the overexpression and secretion of a Rhizopus oryzae lipase (Rol) in P. pastoris was further analyzed. This protein has been previously shown to trigger the unfolded protein response in P. pastoris. A series of 13C-tracer experiments were performed on aerobic chemostat cultivations with a control and two different Rol producing strains growing at a dilution rate of 0.09 h−1 using a glucose:methanol 80:20 (w/w) mix as carbon source. The MFA performed in this study reveals a significant redistristribution of carbon fluxes in the central carbon metabolism when comparing the two recombinant strains vs the control strain, reflected in increased glycolytic, TCA cycle and NADH regeneration fluxes, as well as higher methanol dissimilation rates. Conclusions Overall, a further 13C-based MFA development to characterise the central metabolism of methylotrophic yeasts when growing on mixed

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Directory of Open Access Journals (Sweden)

Jordà Joel

2012-05-01

Full Text Available Abstract Background The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as sole carbon source have been shown to improve product productivities and alleviate metabolic burden derived from protein production. Nevertheless, systematic quantitative studies on the relationships between the central metabolism and recombinant protein production in P. pastoris are still rather limited, particularly when growing this yeast on mixed carbon sources, thus hampering future metabolic network engineering strategies for improved protein production. Results The metabolic flux distribution in the central metabolism of P. pastoris growing on a mixed feed of glucose and methanol was analyzed by Metabolic Flux Analysis (MFA using 13C-NMR-derived constraints. For this purpose, we defined new flux ratios for methanol assimilation pathways in P. pastoris cells growing on glucose:methanol mixtures. By using this experimental approach, the metabolic burden caused by the overexpression and secretion of a Rhizopus oryzae lipase (Rol in P. pastoris was further analyzed. This protein has been previously shown to trigger the unfolded protein response in P. pastoris. A series of 13C-tracer experiments were performed on aerobic chemostat cultivations with a control and two different Rol producing strains growing at a dilution rate of 0.09 h−1 using a glucose:methanol 80:20 (w/w mix as carbon source. The MFA performed in this study reveals a significant redistristribution of carbon fluxes in the central carbon metabolism when comparing the two recombinant strains vs the control strain, reflected in increased glycolytic, TCA cycle and NADH regeneration fluxes, as well as higher methanol dissimilation rates. Conclusions Overall, a further 13C-based MFA development to characterise the central metabolism of methylotrophic

The nutritional status of Methanosarcina acetivorans regulates glycogen metabolism and gluconeogenesis and glycolysis fluxes.

Science.gov (United States)

Santiago-Martínez, Michel Geovanni; Encalada, Rusely; Lira-Silva, Elizabeth; Pineda, Erika; Gallardo-Pérez, Juan Carlos; Reyes-García, Marco Antonio; Saavedra, Emma; Moreno-Sánchez, Rafael; Marín-Hernández, Alvaro; Jasso-Chávez, Ricardo

2016-05-01

Gluconeogenesis is an essential pathway in methanogens because they are unable to use exogenous hexoses as carbon source for cell growth. With the aim of understanding the regulatory mechanisms of central carbon metabolism in Methanosarcina acetivorans, the present study investigated gene expression, the activities and metabolic regulation of key enzymes, metabolite contents and fluxes of gluconeogenesis, as well as glycolysis and glycogen synthesis/degradation pathways. Cells were grown with methanol as a carbon source. Key enzymes were kinetically characterized at physiological pH/temperature. Active consumption of methanol during exponential cell growth correlated with significant methanogenesis, gluconeogenic flux and steady glycogen synthesis. After methanol exhaustion, cells reached the stationary growth phase, which correlated with the rise in glycogen consumption and glycolytic flux, decreased methanogenesis, negligible acetate production and an absence of gluconeogenesis. Elevated activities of carbon monoxide dehydrogenase/acetyl-CoA synthetase complex and pyruvate: ferredoxin oxidoreductase suggested the generation of acetyl-CoA and pyruvate for glycogen synthesis. In the early stationary growth phase, the transcript contents and activities of pyruvate phosphate dikinase, fructose 1,6-bisphosphatase and glycogen synthase decreased, whereas those of glycogen phosphorylase, ADP-phosphofructokinase and pyruvate kinase increased. Therefore, glycogen and gluconeogenic metabolites were synthesized when an external carbon source was provided. Once such a carbon source became depleted, glycolysis and methanogenesis fed by glycogen degradation provided the ATP supply. Weak inhibition of key enzymes by metabolites suggested that the pathways evaluated were mainly transcriptionally regulated. Because glycogen metabolism and glycolysis/gluconeogenesis are not present in all methanogens, the overall data suggest that glycogen storage might represent an environmental

Comparison of annual dry and wet deposition fluxes of selected pesticides in Strasbourg, France

International Nuclear Information System (INIS)

Sauret, Nathalie; Wortham, Henri; Strekowski, Rafal; Herckes, Pierre; Nieto, Laura Ines

2009-01-01

This work summarizes the results of a study of atmospheric wet and dry deposition fluxes of Deisopropyl-atrazine (DEA), Desethyl-atrazine (DET), Atrazine, Terbuthylazine, Alachlor, Metolachlor, Diflufenican, Fenoxaprop-p-ethyl, Iprodione, Isoproturon and Cymoxanil pesticides conducted in Strasbourg, France, from August 2000 through August 2001. The primary objective of this work was to calculate the total atmospheric pesticide deposition fluxes induced by atmospheric particles. To do this, a modified one-dimensional cloud water deposition model was used. All precipitation and deposition samples were collected at an urban forested park environment setting away from any direct point pesticide sources. The obtained deposition fluxes induced by atmospheric particles over a forested area showed that the dry deposition flux strongly contributes to the total deposition flux. The dry particle deposition fluxes are shown to contribute from 4% (DET) to 60% (cymoxanil) to the total deposition flux (wet + dry). - A modified one-dimensional cloud water deposition model is used to estimate the deposition fluxes of pesticides in the particle phase and compare the relative importance of dry and wet depositions

Comparison of annual dry and wet deposition fluxes of selected pesticides in Strasbourg, France

Energy Technology Data Exchange (ETDEWEB)

Sauret, Nathalie [Marseilles University, Laboratoire Chimie Provence - UMR 6264, Campus Saint Charles, Case 29, 3 Place Victor Hugo, 13331 Marseilles Cedex 03 (France); Wortham, Henri [Marseilles University, Laboratoire Chimie Provence - UMR 6264, Campus Saint Charles, Case 29, 3 Place Victor Hugo, 13331 Marseilles Cedex 03 (France)], E-mail: Henri.Wortham@univ-provence.fr; Strekowski, Rafal [Marseilles University, Laboratoire Chimie Provence - UMR 6264, Campus Saint Charles, Case 29, 3 Place Victor Hugo, 13331 Marseilles Cedex 03 (France); Herckes, Pierre [Arizona State University, Department of Chemistry and Biochemistry, Tempe, AZ 85287-1604 (United States); Nieto, Laura Ines [Marseilles University, Laboratoire Chimie Provence - UMR 6264, Campus Saint Charles, Case 29, 3 Place Victor Hugo, 13331 Marseilles Cedex 03 (France)

2009-01-15

This work summarizes the results of a study of atmospheric wet and dry deposition fluxes of Deisopropyl-atrazine (DEA), Desethyl-atrazine (DET), Atrazine, Terbuthylazine, Alachlor, Metolachlor, Diflufenican, Fenoxaprop-p-ethyl, Iprodione, Isoproturon and Cymoxanil pesticides conducted in Strasbourg, France, from August 2000 through August 2001. The primary objective of this work was to calculate the total atmospheric pesticide deposition fluxes induced by atmospheric particles. To do this, a modified one-dimensional cloud water deposition model was used. All precipitation and deposition samples were collected at an urban forested park environment setting away from any direct point pesticide sources. The obtained deposition fluxes induced by atmospheric particles over a forested area showed that the dry deposition flux strongly contributes to the total deposition flux. The dry particle deposition fluxes are shown to contribute from 4% (DET) to 60% (cymoxanil) to the total deposition flux (wet + dry). - A modified one-dimensional cloud water deposition model is used to estimate the deposition fluxes of pesticides in the particle phase and compare the relative importance of dry and wet depositions.

Glycolysis is governed by growth regime and simple enzyme regulation in adherent MDCK cells.

Science.gov (United States)

Rehberg, Markus; Ritter, Joachim B; Reichl, Udo

2014-10-01

Due to its vital importance in the supply of cellular pathways with energy and precursors, glycolysis has been studied for several decades regarding its capacity and regulation. For a systems-level understanding of the Madin-Darby canine kidney (MDCK) cell metabolism, we couple a segregated cell growth model published earlier with a structured model of glycolysis, which is based on relatively simple kinetics for enzymatic reactions of glycolysis, to explain the pathway dynamics under various cultivation conditions. The structured model takes into account in vitro enzyme activities, and links glycolysis with pentose phosphate pathway and glycogenesis. Using a single parameterization, metabolite pool dynamics during cell cultivation, glucose limitation and glucose pulse experiments can be consistently reproduced by considering the cultivation history of the cells. Growth phase-dependent glucose uptake together with cell-specific volume changes generate high intracellular metabolite pools and flux rates to satisfy the cellular demand during growth. Under glucose limitation, the coordinated control of glycolytic enzymes re-adjusts the glycolytic flux to prevent the depletion of glycolytic intermediates. Finally, the model's predictive power supports the design of more efficient bioprocesses.

Modelling drug flux through microporated skin.

Science.gov (United States)

Rzhevskiy, Alexey S; Guy, Richard H; Anissimov, Yuri G

2016-11-10

A simple mathematical equation has been developed to predict drug flux through microporated skin. The theoretical model is based on an approach applied previously to water evaporation through leaf stomata. Pore density, pore radius and drug molecular weight are key model parameters. The predictions of the model were compared with results derived from a simple, intuitive method using porated area alone to estimate the flux enhancement. It is shown that the new approach predicts significantly higher fluxes than the intuitive analysis, with transport being proportional to the total pore perimeter rather than area as intuitively anticipated. Predicted fluxes were in good general agreement with experimental data on drug delivery from the literature, and were quantitatively closer to the measured values than those derived from the intuitive, area-based approach. Copyright © 2016 Elsevier B.V. All rights reserved.

The potentiation of radiation effects on the Meth-a and RIF tumors and hela s-3 cells by the glycolytic inhibitor, Lonidamine

International Nuclear Information System (INIS)

Kim, J.H.; Alfieri, A.A.; Kim, S.H.; He, S.Q.; Young, C.W.

1984-01-01

The indazole carboxylic acid Lonidamine (L) a glycolytic inhibitor and antispermatogenic agent previously found to have hyperthermia sensitization properties was evaluated as a radiopotentiating agent in vitro and in vivo. Tumoricidal effects of (L) i.p. and single dose x-irradiation (3.0 or 3.2 Gy) was evaluated on 300-1000 mm/sup 3/ i.m. Meth-A in BALB/c and the RIF fibrosarcoma in C/sub 3/H/He mice L preceded or proceeded the radiation event to 1 hr intervals with maximum separation between treatments of 4 hrs. L conc in sera was biphasic (t1/2 -- 1 hr.). Optimal treatment efficacy was found at minimal time intervals between L and radiation interaction. TCD /sub 50/ for RIF and Meth-A resulted in DMF's of 1.15 - 1.35 (L dose dependent). Chronic L (50mg/kg/dy x 30) + fractionated radiation was found to be well tolerated as concomitant L potentiated combination therapy. Cell culture studies on monolayer and spheroids demonstrate L as an effective inhibitor of potential lethal radiation damage with L potential to be dependent upon both dose/fraction, number of fractions and total radiation dose accumulated

Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function

DEFF Research Database (Denmark)

Jacobs, Robert A; Díaz, Víctor; Soldini, Lavinia

2013-01-01

The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirom......The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high......-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks......) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085...

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Science.gov (United States)

Thwe, Phyu M; Pelgrom, Leonard; Cooper, Rachel; Beauchamp, Saritha; Reisz, Julie A; D'Alessandro, Angelo; Everts, Bart; Amiel, Eyal

2017-09-05

Dendritic cell (DC) activation by Toll-like receptor (TLR) agonists causes rapid glycolytic reprogramming that is required to meet the metabolic demands of their immune activation. Recent efforts in the field have identified an important role for extracellular glucose sourcing to support DC activation. However, the contributions of intracellular glucose stores to these processes have not been well characterized. We demonstrate that DCs possess intracellular glycogen stores and that cell-intrinsic glycogen metabolism supports the early effector functions of TLR-activated DCs. Inhibition of glycogenolysis significantly attenuates TLR-mediated DC maturation and impairs their ability to initiate lymphocyte activation. We further report that DCs exhibit functional compartmentalization of glucose- and glycogen-derived carbons, where these substrates preferentially contribute to distinct metabolic pathways. This work provides novel insights into nutrient homeostasis in DCs, demonstrating that differential utilization of glycogen and glucose metabolism regulates their optimal immune function. Copyright © 2017 Elsevier Inc. All rights reserved.

Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon

Directory of Open Access Journals (Sweden)

Sylvia A. Reimann

2011-01-01

Full Text Available Bacteria adapt to changing environments by means of tightly coordinated regulatory circuits. The use of synthetic lethality, a genetic phenomenon in which the combination of two nonlethal mutations causes cell death, facilitates identification and study of such circuitry. In this study, we show that the E. coli ompR malTcon double mutant exhibits a synthetic lethal phenotype that is environmentally conditional. MalTcon, the constitutively active form of the maltose system regulator MalT, causes elevated expression of the outer membrane porin LamB, which leads to death in the absence of the osmoregulator OmpR. However, the presence and metabolism of glycolytic carbon sources, such as sorbitol, promotes viability and unveils a novel layer of regulation within the complex circuitry that controls maltose transport and metabolism.

Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

International Nuclear Information System (INIS)

Campos, Dijon Henrique Salomé de; Leopoldo, André Soares; Lima-Leopoldo, Ana Paula; Nascimento, André Ferreira do; Oliveira-Junior, Silvio Assis de; Silva, Danielle Cristina Tomaz da; Sugizaki, Mario Mateus; Padovani, Carlos Roberto; Cicogna, Antonio Carlos

2014-01-01

Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV). Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle

Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

Energy Technology Data Exchange (ETDEWEB)

Campos, Dijon Henrique Salomé de, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Leopoldo, André Soares; Lima-Leopoldo, Ana Paula [Departamento de Esportes - Centro de Educação Física e Desportos da Universidade Federal do Espírito Santo (UFES), Vitória, ES (Brazil); Nascimento, André Ferreira do [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Oliveira-Junior, Silvio Assis de [Escola de Fisioterapia da Universidade Federal do Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil); Silva, Danielle Cristina Tomaz da [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Sugizaki, Mario Mateus [Instituto de Ciências da Saúde da Universidade Federal do Mato Grosso (UFMT), Sinop, MT (Brazil); Padovani, Carlos Roberto [Departamento de Bioestatística, Instituto de Ciências Biológicas da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil); Cicogna, Antonio Carlos, E-mail: dijoncampos@gmail.com [Departamento de Clínica Médica - Faculdade de Medicina de Botucatu da Universidade Estadual Paulista (UNESP), Botucatu, SP (Brazil)

2014-10-15

Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV). Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

Updates to a 13C metabolic flux analysis model for evaluating energy metabolism in cultured cerebellar granule neurons from neonatal rats.

Science.gov (United States)

Jekabsons, Mika B; Gebril, Hoda M; Wang, Yan-Hong; Avula, Bharathi; Khan, Ikhlas A

2017-10-01

A hexose phosphate recycling model previously developed to infer fluxes through the major glucose consuming pathways in cultured cerebellar granule neurons (CGNs) from neonatal rats metabolizing [1,2- 13 C 2 ]glucose was revised by considering reverse flux through the non-oxidative pentose phosphate pathway (PPP) and symmetrical succinate oxidation within the tricarboxylic acid (TCA) cycle. The model adjusts three flux ratios to effect 13 C distribution in the hexose, pentose, and triose phosphate pools, and in TCA cycle malate to minimize the error between predicted and measured 13 C labeling in exported lactate (i.e., unlabeled, single-, double-, and triple-labeled; M, M1, M2, and M3, respectively). Inclusion of reverse non-oxidative PPP flux substantially increased the number of calculations but ultimately had relatively minor effects on the labeling of glycolytic metabolites. From the error-minimized solution in which the predicted M-M3 lactate differed by 0.49% from that measured by liquid chromatography-triple quadrupole mass spectrometry, the neurons exhibited negligible forward non-oxidative PPP flux. Thus, no glucose was used by the pentose cycle despite explicit consideration of hexose phosphate recycling. Mitochondria consumed only 16% of glucose while 45% was exported as lactate by aerobic glycolysis. The remaining 39% of glucose was shunted to pentose phosphates presumably for de novo nucleotide synthesis, but the proportion metabolized through the oxidative PPP vs. the reverse non-oxidative PPP could not be determined. The lactate exported as M1 (2.5%) and M3 (1.2%) was attributed to malic enzyme, which was responsible for 7.8% of pyruvate production (vs. 92.2% by glycolysis). The updated model is more broadly applicable to different cell types by considering bi-directional flux through the non-oxidative PPP. Its application to cultured neurons utilizing glucose as the sole exogenous substrate has demonstrated substantial oxygen-independent glucose

High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4+ T cells in both mice and humans.

Science.gov (United States)

Manfrini, Nicola; Ricciardi, Sara; Miluzio, Annarita; Fedeli, Maya; Scagliola, Alessandra; Gallo, Simone; Brina, Daniela; Adler, Thure; Busch, Dirk H; Gailus-Durner, Valerie; Fuchs, Helmut; Hrabě de Angelis, Martin; Biffo, Stefano

2017-12-01

Eukaryotic Initiation Factor 6 (eIF6) is required for 60S ribosomal subunit biogenesis and efficient initiation of translation. Intriguingly, in both mice and humans, endogenous levels of eIF6 are detrimental as they act as tumor and obesity facilitators, raising the question on the evolutionary pressure that maintains high eIF6 levels. Here we show that, in mice and humans, high levels of eIF6 are required for proper immune functions. First, eIF6 heterozygous (het) mice show an increased mortality during viral infection and a reduction of peripheral blood CD4 + Effector Memory T cells. In human CD4 + T cells, eIF6 levels rapidly increase upon T-cell receptor activation and drive the glycolytic switch and the acquisition of effector functions. Importantly, in CD4 + T cells, eIF6 levels control interferon-γ (IFN-γ) secretion without affecting proliferation. In conclusion, the immune system has a high evolutionary pressure for the maintenance of a dynamic and powerful regulation of the translational machinery. Copyright © 2017 Elsevier Ltd. All rights reserved.

Gap-related trapped magnetic flux dependence between single and combined bulk superconductors

International Nuclear Information System (INIS)

Deng, Z.; Miki, M.; Felder, B.; Tsuzuki, K.; Shinohara, N.; Uetake, T.; Izumi, M.

2011-01-01

Highlights: → Rectangular YBCO bulks to realize a compact combination. → The gap effect was added to consider in the trapped flux density mapping. → The trapped-flux dependence between single and combined bulks is gap related. → It is possible to estimate the total magnetic flux of bulk combinations. - Abstract: Aiming at examining the trapped-flux dependence between single and combined bulk superconductors for field-pole applications, three rectangular Y 1.65 Ba 2 Cu 3 O 7-x (YBCO) bulks with a possibly compact combination were employed to investigate the trapped-flux characteristics of single and combined bulks with a field-cooling magnetization (FCM) method. A gap-related dependence was found between them. At lower gaps of 1 mm and 5 mm, the peak trapped fields and total magnetic flux of combined bulks are both smaller than the additive values of each single bulk, which can be ascribed to the demagnetization influences of the field around the bulk generated by the adjacent ones. While, at larger gaps like 10 mm, the situation becomes reversed. The combined bulks can attain bigger peak trapped fields as well as total magnetic flux, which indicates that the magnetic field by the bulk combination can reach higher gaps, thanks to the bigger magnetic energy compared with the single bulk. The presented results show that, on one hand, it is possible to estimate the total trapped magnetic flux of combined bulks by an approximate additive method of each single bulk while considering a demagnetization factor; on the other hand, it also means that the performance of combined bulks will be superior to the addition of each single bulk at larger gaps, thus preferable for large-scaled magnet applications.

Gap-related trapped magnetic flux dependence between single and combined bulk superconductors

Energy Technology Data Exchange (ETDEWEB)

Deng, Z., E-mail: zgdeng@gmail.co [Laboratory of Applied Physics, Department of Marine Electronics and Mechanical Engineering, Tokyo University of Marine Science and Technology, Tokyo 135-8533 (Japan); Miki, M.; Felder, B.; Tsuzuki, K.; Shinohara, N.; Uetake, T.; Izumi, M. [Laboratory of Applied Physics, Department of Marine Electronics and Mechanical Engineering, Tokyo University of Marine Science and Technology, Tokyo 135-8533 (Japan)

2011-05-15

Highlights: {yields} Rectangular YBCO bulks to realize a compact combination. {yields} The gap effect was added to consider in the trapped flux density mapping. {yields} The trapped-flux dependence between single and combined bulks is gap related. {yields} It is possible to estimate the total magnetic flux of bulk combinations. - Abstract: Aiming at examining the trapped-flux dependence between single and combined bulk superconductors for field-pole applications, three rectangular Y{sub 1.65}Ba{sub 2}Cu{sub 3}O{sub 7-x} (YBCO) bulks with a possibly compact combination were employed to investigate the trapped-flux characteristics of single and combined bulks with a field-cooling magnetization (FCM) method. A gap-related dependence was found between them. At lower gaps of 1 mm and 5 mm, the peak trapped fields and total magnetic flux of combined bulks are both smaller than the additive values of each single bulk, which can be ascribed to the demagnetization influences of the field around the bulk generated by the adjacent ones. While, at larger gaps like 10 mm, the situation becomes reversed. The combined bulks can attain bigger peak trapped fields as well as total magnetic flux, which indicates that the magnetic field by the bulk combination can reach higher gaps, thanks to the bigger magnetic energy compared with the single bulk. The presented results show that, on one hand, it is possible to estimate the total trapped magnetic flux of combined bulks by an approximate additive method of each single bulk while considering a demagnetization factor; on the other hand, it also means that the performance of combined bulks will be superior to the addition of each single bulk at larger gaps, thus preferable for large-scaled magnet applications.

Comprehensive Method for Culturing Embryonic Dorsal Root Ganglion Neurons for Seahorse Extracellular Flux XF24 Analysis.

Science.gov (United States)

Lange, Miranda; Zeng, Yan; Knight, Andrew; Windebank, Anthony; Trushina, Eugenia

2012-01-01

Changes in mitochondrial dynamics and function contribute to progression of multiple neurodegenerative diseases including peripheral neuropathies. The Seahorse Extracellular Flux XF24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in assessing mitochondrial function. One of the most important steps in the analysis of mitochondrial respiration using the Seahorse XF24 analyzer is plating a uniform monolayer of firmly attached cells. However, culturing of primary dorsal root ganglion (DRG) neurons is associated with multiple challenges, including their propensity to form clumps and detach from the culture plate. This could significantly interfere with proper analysis and interpretation of data. We have tested multiple cell culture parameters including coating substrates, culture medium, XF24 microplate plastics, and plating techniques in order to optimize plating conditions. Here we describe a highly reproducible method to obtain neuron-enriched monolayers of securely attached dissociated primary embryonic (E15) rat DRG neurons suitable for analysis with the Seahorse XF24 platform.

Comprehensive method for culturing embryonic dorsal root ganglion neurons for Seahorse Extracellular Flux XF24 Analysis

Directory of Open Access Journals (Sweden)

Miranda L. Lange

2012-12-01

Full Text Available Changes in mitochondrial dynamics and function contribute to progression of multiple neurodegenerative diseases including peripheral neuropathies. The Seahorse Extracellular Flux XF24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in assessing mitochondrial function. One of the most important steps in the analysis of mitochondrial respiration using the Seahorse XF24 analyzer is plating a uniform monolayer of firmly attached cells. However, culturing of primary dorsal root ganglion (DRG neurons is associated with multiple challenges, including their propensity to form clumps and detach from the culture plate. This could significantly interfere with proper analysis and interpretation of data. We have tested multiple cell culture parameters including coating substrates, culture medium, XF24 microplate plastics, and plating techniques in order to optimize plating conditions. Here we describe a highly reproducible method to obtain neuron-enriched monolayers of securely attached dissociated primary embryonic (E15 rat DRG neurons suitable for analysis with the Seahorse XF24 platform.

Turbulent Fogwater Flux Measurements Above A Forest

Science.gov (United States)

Burkard, R.; Eugster, W.; Buetzberger, P.; Siegwolf, R.

Many forest ecosystems in elevated regions receive a significant fraction of their wa- ter and nutrient input by the interception of fogwater. Recently, several studies have demonstrated the suitability of the eddy covariance technique for the direct measure- ment of turbulent liquid water fluxes. Since summer 2001 a fogwater flux measure- ment equipment has been running at a montane site above a mixed forest canopy in Switzerland. The measurement equipment consists of a high-speed size-resolving droplet spectrometer and a three-dimensional ultrasonic anemometer. The chemical composition of the fogwater was determined from samples collected with a modified Caltech active strand collector. The deposition of nutrients by fog (occult deposition) was calculated by multiplying the total fogwater flux (total of measured turbulent and calculated gravitational flux) during each fog event by the ionic concentrations found in the collected fogwater. Several uncertainties still exist as far as the accuracy of the measurements is con- cerned. Although there is no universal statistical approach for testing the quality of the liquid water flux data directly, results of independent data quality checks of the two time series involved in the flux computation and accordingly the two instruments (ultrasonic anemometer and the droplet spectrometer) are presented. Within the measurement period, over 80 fog events with a duration longer than 2.5 hours were analyzed. An enormous physical and chemical heterogeneity among these fog events was found. We assume that some of this heterogeneity is due to the fact that fog or cloud droplets are not conservative entities: the turbulent flux of fog droplets, which can be referred to as the liquid water flux, is affected by phase change processes and coagulation. The measured coexistence of upward fluxes of small fog droplets (di- ameter < 10 µm) with the downward transport of larger droplets indicates the influ- ence of such processes. With the

Inverse modeling of the terrestrial carbon flux in China with flux covariance among inverted regions

Science.gov (United States)

Wang, H.; Jiang, F.; Chen, J. M.; Ju, W.; Wang, H.

2011-12-01

Quantitative understanding of the role of ocean and terrestrial biosphere in the global carbon cycle, their response and feedback to climate change is required for the future projection of the global climate. China has the largest amount of anthropogenic CO2 emission, diverse terrestrial ecosystems and an unprecedented rate of urbanization. Thus information on spatial and temporal distributions of the terrestrial carbon flux in China is of great importance in understanding the global carbon cycle. We developed a nested inversion with focus in China. Based on Transcom 22 regions for the globe, we divide China and its neighboring countries into 17 regions, making 39 regions in total for the globe. A Bayesian synthesis inversion is made to estimate the terrestrial carbon flux based on GlobalView CO2 data. In the inversion, GEOS-Chem is used as the transport model to develop the transport matrix. A terrestrial ecosystem model named BEPS is used to produce the prior surface flux to constrain the inversion. However, the sparseness of available observation stations in Asia poses a challenge to the inversion for the 17 small regions. To obtain additional constraint on the inversion, a prior flux covariance matrix is constructed using the BEPS model through analyzing the correlation in the net carbon flux among regions under variable climate conditions. The use of the covariance among different regions in the inversion effectively extends the information content of CO2 observations to more regions. The carbon flux over the 39 land and ocean regions are inverted for the period from 2004 to 2009. In order to investigate the impact of introducing the covariance matrix with non-zero off-diagonal values to the inversion, the inverted terrestrial carbon flux over China is evaluated against ChinaFlux eddy-covariance observations after applying an upscaling methodology.

Neutron flux stabilization in the NG-150 neutron generators

International Nuclear Information System (INIS)

Kuz'min, L.E.; Makarov, S.A.; Pronman, I.M.

1986-01-01

Problem of metal tritium target lifetime increase and neutron flux stabilization in the NG-150 neutron generators is studied. Possibility on neutron flux stabilization using the mass analyzer for low-angle (4 deg and 41 deg) mass separation of a beam in thre components, which fall on a target simultaneously, is confirmed experimentally. Basic generator parameters are: accelerating voltage of 150 kV, total beam current on a target of 1.5 mA, beam current density of 0.3-1.6 mA/cm 2 , beam diameter of 8 mm. The initial neutron flux on the targets of 0.73 mg/cm 2 thick constituted 1.1x10 11 ssup(-1). The neutron flux monitoring was accomplished from recoil proton recording by a plastic scintillator. Flux decrease by more than 5% served as a signel for measuring mass analyzer magnetic field providing beam displacement on a target and restoration of the given flux. The NG-150 generator neutron flux stabilization was attained during 2h

Methane Flux of Amazonian Peatland Ecosystems: Large Ecosystem Fluxes with Substantial Contribution from Palm (maritia Flexuosa) STEM Emissions

Science.gov (United States)

Van Haren, J. L. M.; Cadillo-Quiroz, H.

2015-12-01

Methane (CH4) emissions through plants have long been known in wetlands. However, most measurements have focused on stem tops and leaves. Recently, measurements at the lower parts of stems have shown that stem emissions can exceed soil CH4 emissions in Asian peatlands (Pangala et al. 2013). The addition of stem fluxes to soil fluxes for total ecosystem fluxes has the potential to bridge the discrepancy between modeled to measured and bottom-up to top-down flux estimates. Our measurements in peatlands of Peru show that especially Mauritia flexuosa, a palm species, can emit very large quantities of CH4, although most trees emitted at least some CH4. We used flexible stem chambers to adapt to stems of any size above 5cm in diameter. The chambers were sampled in closed loop with a Gasmet DX4015 for flux measurements, which lasted ~5 minutes after flushing with ambient air. We found that M. flexuosa stem fluxes decrease with height along the stem and were positively correlated with soil fluxes. Most likely CH4 is transported up the stem with the xylem water. Measured M. flexuosa stem fluxes below 1.5m averaged 11.2±1.5 mg-C m-2 h-1 (±95% CI) with a maximum of 123±3.5 mg-C m-2 h-1 (±SE), whereas soil fluxes averaged 6.7±1.7 mg-C m-2 h-1 (±95% CI) with a maximum of 31.6±0.4 mg-C m-2 h-1 (±SE). Significant CH4 fluxes were measured up to 5 m height along the stems. Combined with the high density of ~150 M. flexuosa individuals per hectare in these peatlands and the consistent diameter of ~30cm, the high flux rates add ~20% to the soil flux. With anywhere between 1 and 5 billion M. flexuosa stems across Amazon basin wetlands, stem fluxes from this palm species could represent a major addition to the overall Amazon basin CH4 flux.

Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae

DEFF Research Database (Denmark)

Vemuri, Goutham; Eiteman, M.A; McEwen, J.E

2007-01-01

effect is due to limited respiratory capacity or is caused by glucose-mediated repression of respiration. When respiration in S. cerevisiae was increased by introducing a heterologous alternative oxidase, we observed reduced aerobic ethanol formation. In contrast, increasing nonrespiratory NADH oxidation...... Crabtree effect.’’ The yeast Saccharomyces cerevisiae has served as an important model organism for studying the Crabtree effect. When subjected to increasing glycolytic fluxes under aerobic conditions, there is a threshold value of the glucose uptake rate at which the metabolism shifts from purely...... respiratory to mixed respiratory and fermentative. It is well known that glucose repression of respiratory pathways occurs at high glycolytic fluxes, resulting in a decrease in respiratory capacity. Despite many years of detailed studies on this subject, it is not known whether the onset of the Crabtree...

Insulin secretion and cellular glucose metabolism after prolonged low-grade intralipid infusion in young men

DEFF Research Database (Denmark)

Jensen, Christine B; Storgaard, Heidi; Holst, Jens J

2003-01-01

We examined the simultaneous effects of a 24-h low-grade Intralipid infusion on peripheral glucose disposal, intracellular glucose partitioning and insulin secretion rates in twenty young men, by 2-step hyperinsulinemic euglycemic clamp [low insulin clamp (LI), 10 mU/m(2) x min; high insulin clamp...... Intralipid infusion. At LI, glucose oxidation decreased by 10%, whereas glucose disposal, glycolytic flux, glucose storage, and glucose production were not significantly altered. At HI, glucose disposal, and glucose oxidation decreased by 12% and 24%, respectively, during Intralipid infusion. Glycolytic flux......, glucose storage, and glucose production were unchanged. Insulin secretion rates increased in response to Intralipid infusion, but disposition indices (DI = insulin action.insulin secretion) were unchanged. In conclusion, a 24-h low-grade Intralipid infusion caused insulin resistance in the oxidative (but...

Carbon Fluxes and Transport Along the Terrestrial Aquatic Continuum

Science.gov (United States)

Butman, D. E.; Kolka, R.; Fennel, K.; Stackpoole, S. M.; Trettin, C.; Windham-Myers, L.

2017-12-01

Terrestrial wetlands, inland surface waters, tidal wetlands and estuaries, and the coastal ocean are distinct aquatic ecosystems that integrate carbon (C) fluxes and processing among the major earth system components: the continents, oceans, and atmosphere. The development of the 2nd State of the Carbon Cycle Report (SOCCR2) noted that incorporating the C cycle dynamics for these ecosystems was necessary to reconcile some of the gaps associated with the North American C budget. We present major C stocks and fluxes for Canada, Mexico and the United States. North America contains nearly 42% of the global terrestrial wetland area. Terrestrial wetlands, defined as soils that are seasonally or permanently inundated or saturated, contain significant C stocks equivalent to 174,000 Tg C in the top 40 cm of soil. While terrestrial wetlands are a C sink of approximately 64 Tg C yr-1, they also emit 21 Tg of CH4 yr-1. Inland waters are defined as lakes, reservoirs, rivers, and streams. Carbon fluxes, which include lateral C export to the coast, riverine and lacustrine CO2 emissions, and C burial in lakes and reservoirs are estimated at 507 Tg yr-1. Estuaries and tidal wetlands assimilate C and nutrients from uplands and rivers, and their total C stock is 1,323 Tg C in the top 1 m of soils and sediment. Accounting for soil accretion, lateral C flux, and CO2 assimilation and emission, tidal wetlands and estuaries are net sinks with a total flux equal to 6 Tg C yr-1. The coastal ocean and sea shelfs, defined as non-estuarine waters within 200 nautical miles (370 km) of the coast, function as net sinks, with the air-sea exchange of CO2 estimated at 150 Tg C yr-1. In total, fluxes from these four aquatic ecosystems are equal to a loss of 302 Tg C yr-1. Including these four discrete fluxes in this assessment demonstrates the importance of linking hydrology and biogeochemical cycling to evaluate the impacts of climate change and human activities on carbon fluxes across the

Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway

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Dijon Henrique Salomé de Campos

2014-10-01

Full Text Available Background: Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Objective: Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced glucose leads to cardiac dysfunction in obesity. Methods: 30-day-old male Wistar rats were fed standard and hypercaloric diet for 30 weeks. Cardiac function and morphology were assessed. In this paper was viewed the general characteristics and comorbities associated to obesity. The structure cardiac was determined by weights of the heart and left ventricle (LV. Myocardial function was evaluated by studying isolated papillary muscles from the LV, under the baseline condition and after inotropic and lusitropic maneuvers: myocardial stiffness; postrest contraction; increase in extracellular Ca2+ concentration; change in heart rate and inhibitor of glycolytic pathway. Results: Compared with control group, the obese rats had increased body fat and co-morbities associated with obesity. Functional assessment after blocking iodoacetate shows no difference in the linear regression of DT, however, the RT showed a statistically significant difference in behavior between the control and the obese group, most notable being the slope in group C. Conclusion: The energy imbalance on obesity did not cause cardiac dysfunction. On the contrary, the prioritization of fatty acids utilization provides protection to cardiac muscle during the inhibition of glycolysis, suggesting that this pathway is fewer used by obese cardiac muscle.

Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

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João Paulo Lopes-Silva

Full Text Available The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation.Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1 or placebo (cellulose one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery, in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR and the estimated contribution of the oxidative (WAER, ATP-PCr (WPCR, and glycolytic (W[La-] systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1 taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s, 2 taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ, or by 3 analyzing the first 30-s via logarithmic regression analysis (T30.Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04. However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48 or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58. Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62, HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12, oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61 and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72 during the combat simulation were unaffected. Furthermore

Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation.

Science.gov (United States)

Lopes-Silva, João Paulo; Silva Santos, Jonatas Ferreira da; Branco, Braulio Henrique Magnani; Abad, César Cavinato Cal; Oliveira, Luana Farias de; Loturco, Irineu; Franchini, Emerson

2015-01-01

The aim of this study was to evaluate the effect of caffeine ingestion on performance and estimated energy system contribution during simulated taekwondo combat and on post-exercise parasympathetic reactivation. Ten taekwondo athletes completed two experimental sessions separated by at least 48 hours. Athletes consumed a capsule containing either caffeine (5 mg∙kg-1) or placebo (cellulose) one hour before the combat simulation (3 rounds of 2 min separated by 1 min passive recovery), in a double-blind, randomized, repeated-measures crossover design. All simulated combat was filmed to quantify the time spent fighting in each round. Lactate concentration and rating of perceived exertion were measured before and after each round, while heart rate (HR) and the estimated contribution of the oxidative (WAER), ATP-PCr (WPCR), and glycolytic (W[La-]) systems were calculated during the combat simulation. Furthermore, parasympathetic reactivation after the combat simulation was evaluated through 1) taking absolute difference between the final HR observed at the end of third round and the HR recorded 60-s after (HRR60s), 2) taking the time constant of HR decay obtained by fitting the 6-min post-exercise HRR into a first-order exponential decay curve (HRRτ), or by 3) analyzing the first 30-s via logarithmic regression analysis (T30). Caffeine ingestion increased estimated glycolytic energy contribution in relation to placebo (12.5 ± 1.7 kJ and 8.9 ± 1.2 kJ, P = 0.04). However, caffeine did not improve performance as measured by attack number (CAF: 26. 7 ± 1.9; PLA: 27.3 ± 2.1, P = 0.48) or attack time (CAF: 33.8 ± 1.9 s; PLA: 36.6 ± 4.5 s, P = 0.58). Similarly, RPE (CAF: 11.7 ± 0.4 a.u.; PLA: 11.5 ± 0.3 a.u., P = 0.62), HR (CAF: 170 ± 3.5 bpm; PLA: 174.2 bpm, P = 0.12), oxidative (CAF: 109.3 ± 4.5 kJ; PLA: 107.9 kJ, P = 0.61) and ATP-PCr energy contributions (CAF: 45.3 ± 3.4 kJ; PLA: 46.8 ± 3.6 kJ, P = 0.72) during the combat simulation were unaffected

Comments on the 'minimum flux corona' concept

International Nuclear Information System (INIS)

Antiochos, S.K.; Underwood, J.H.

1978-01-01

Hearn's (1975) models of the energy balance and mass loss of stellar coronae, based on a 'minimum flux corona' concept, are critically examined. First, it is shown that the neglect of the relevant length scales for coronal temperature variation leads to an inconsistent computation of the total energy flux F. The stability arguments upon which the minimum flux concept is based are shown to be fallacious. Errors in the computation of the stellar wind contribution to the energy budget are identified. Finally we criticize Hearn's (1977) suggestion that the model, with a value of the thermal conductivity modified by the magnetic field, can explain the difference between solar coronal holes and quiet coronal regions. (orig.) 891 WL [de

Direct evidence for activity-dependent glucose phosphorylation in neurons with implications for the astrocyte-to-neuron lactate shuttle

OpenAIRE

Patel, Anant B.; Lai, James C. K.; Chowdhury, Golam M. I.; Hyder, Fahmeed; Rothman, Douglas L.; Shulman, Robert G.; Behar, Kevin L.

2014-01-01

A near one-to-one relationship had previously been observed between increments in the fluxes of the glutamate−glutamine neurotransmitter cycle and neuronal glucose oxidation in the tricarboxylic acid (TCA) cycle. This flux relationship was consistent with a hypothesized mechanism involving glycolytic ATP in astrocytes and astrocyte-to-neuron lactate shuttling. Here, 2-fluoro-2-deoxy-d-glucose was used to evaluate the glucose flux through glycolysis and the TCA cycle in nerve terminals isolate...

Characterization of phosphofructokinase activity in Mycobacterium tuberculosis reveals that a functional glycolytic carbon flow is necessary to limit the accumulation of toxic metabolic intermediates under hypoxia.

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Wai Yee Phong

Full Text Available Metabolic versatility has been increasingly recognized as a major virulence mechanism that enables Mycobacterium tuberculosis to persist in many microenvironments encountered in its host. Glucose is one of the most abundant carbon sources that is exploited by many pathogenic bacteria in the human host. M. tuberculosis has an intact glycolytic pathway that is highly conserved in all clinical isolates sequenced to date suggesting that glucose may represent a non-negligible source of carbon and energy for this pathogen in vivo. Fructose-6-phosphate phosphorylation represents the key-committing step in glycolysis and is catalyzed by a phosphofructokinase (PFK activity. Two genes, pfkA and pfkB have been annotated to encode putative PFK in M. tuberculosis. Here, we show that PFKA is the sole PFK enzyme in M. tuberculosis with no functional redundancy with PFKB. PFKA is required for growth on glucose as sole carbon source. In co-metabolism experiments, we report that disruption of the glycolytic pathway at the PFK step results in intracellular accumulation of sugar-phosphates that correlated with significant impairment of the cell viability. Concomitantly, we found that the presence of glucose is highly toxic for the long-term survival of hypoxic non-replicating mycobacteria, suggesting that accumulation of glucose-derived toxic metabolites does occur in the absence of sustained aerobic respiration. The culture medium traditionally used to study the physiology of hypoxic mycobacteria is supplemented with glucose. In this medium, M. tuberculosis can survive for only 7-10 days in a true non-replicating state before death is observed. By omitting glucose in the medium this period could be extended for up to at least 40 days without significant viability loss. Therefore, our study suggests that glycolysis leads to accumulation of glucose-derived toxic metabolites that limits long-term survival of hypoxic mycobacteria. Such toxic effect is exacerbated when

Event-driven sediment flux in Hueneme and Mugu submarine canyons, southern California

Science.gov (United States)

Xu, J. P.; Swarzenski, P.W.; Noble, M.; Li, A.-C.

2010-01-01

Vertical sediment fluxes and their dominant controlling processes in Hueneme and Mugu submarine canyons off south-central California were assessed using data from sediment traps and current meters on two moorings that were deployed for 6 months during the winter of 2007. The maxima of total particulate flux, which reached as high as 300+ g/m2/day in Hueneme Canyon, were recorded during winter storm events when high waves and river floods often coincided. During these winter storms, wave-induced resuspension of shelf sediment was a major source for the elevated sediment fluxes. Canyon rim morphology, rather than physical proximity to an adjacent river mouth, appeared to control the magnitude of sediment fluxes in these two submarine canyon systems. Episodic turbidity currents and internal bores enhanced sediment fluxes, particularly in the lower sediment traps positioned 30 m above the canyon floor. Lower excess 210Pb activities measured in the sediment samples collected during periods of peak total particulate flux further substantiate that reworked shelf-, rather than newly introduced river-borne, sediments supply most of the material entering these canyons during storms.

Ozone flux of an urban orange grove: multiple scaled measurements and model comparisons

Science.gov (United States)

Alstad, K. P.; Grulke, N. E.; Jenerette, D. G.; Schilling, S.; Marrett, K.

2009-12-01

There is significant uncertainty about the ozone sink properties of the phytosphere due to a complexity of interactions and feedbacks with biotic and abiotic factors. Improved understanding of the controls on ozone fluxes is critical to estimating and regulating the total ozone budget. Ozone exchanges of an orange orchard within the city of Riverside, CA were examined using a multiple-scaled approach. We access the carbon, water, and energy budgets at the stand- to leaf- level to elucidate the mechanisms controlling the variability in ozone fluxes of this agro-ecosystem. The two initial goals of the study were 1. To consider variations and controls on the ozone fluxes within the canopy; and, 2. To examine different modeling and scaling approaches for totaling the ozone fluxes of this orchard. Current understanding of the total ozone flux between the atmosphere near ground and the phytosphere (F-total) include consideration of a fraction which is absorbed by vegetation through stomatal uptake (F-absorb), and fractional components of deposition on external, non-stomatal, surfaces of the vegetation (F-external) and soil (F-soil). Multiplicative stomatal-conductance models have been commonly used to estimate F-absorb, since this flux cannot be measured directly. We approach F-absorb estimates for this orange orchard using chamber measurement of leaf stomatal-conductance, as well as non-chamber sap-conductance collected on branches of varied aspect and sun/shade conditions within the canopy. We use two approaches to measure the F-total of this stand. Gradient flux profiles were measured using slow-response ozone sensors collecting within and above the canopy (4.6 m), and at the top of the tower (8.5 m). In addition, an eddy-covariance system fitted with a high-frequency chemiluminescence ozone system will be deployed (8.5 m). Preliminary ozone gradient flux profiles demonstrate a substantial ozone sink strength of this orchard, with diurnal concentration differentials

Effect of axial heat flux distribution on CHF

Energy Technology Data Exchange (ETDEWEB)

Park, Cheol

2000-10-01

Previous investigations for the effect of axial heat flux distributions on CHF and the prediction methods are reviewed and summarized. A total of 856 CHF data in a tube with a non-uniform axial heat flux distribution has been compiled from the articles and analyzed using the 1995 Groeneveld look-up table. The results showed that two representative correction factors, K5 of the look-up table and Tongs F factor, can be applied to describe the axial heat flux distribution effect on CHF. However, they overpredict slightly the measured CHF, depending on the quality and flux peak shape. Hence, a corrected K5 factor, which accounts for the axial heat flux distribution effect is suggested to correct these trends. It predicted the CHF power for the compiled data with an average error of 1.5% and a standard deviation of 10.3%, and also provides a reasonable prediction of CHF locations.

Nitrates in Groundwater Discharges from the Azores Archipelago: Occurrence and Fluxes to Coastal Waters

Directory of Open Access Journals (Sweden)

J. Virgílio Cruz

2017-02-01

Full Text Available Groundwater discharge is an important vector of chemical fluxes to the ocean environment, and as the concentration of nutrients is often higher in discharging groundwater, the deterioration of water quality in the receiving environment can be the result. The main objective of the present paper is to estimate the total NO3 flux to coastal water bodies due to groundwater discharge in the volcanic Azores archipelago (Portugal. Therefore, 78 springs discharging from perched-water bodies have been monitored since 2003, corresponding to cold (mean = 14.9 °C and low mineralized (47.2–583 µS/cm groundwater from the sodium-bicarbonate to sodium-chloride water types. A set of 36 wells was also monitored, presenting groundwater with a higher mineralization. The nitrate content in springs range between 0.02 and 37.4 mg/L, and the most enriched samples are associated to the impact of agricultural activities. The total groundwater NO3 flux to the ocean is estimated in the range of 5.23 × 103 to 190.6 × 103 mol/km2/a (∑ = ~523 × 103 mol/km2/a, exceeding the total flux associated to surface runoff (∑ = ~281 × 103 mol/km2/a. In the majority of the islands, the estimated fluxes are higher than runoff fluxes, with the exception of Pico (47.2%, Corvo (46% and Faial (7.2%. The total N-NO3 flux estimated in the Azores (~118.9 × 103 mol/km2/a is in the lower range of estimates made in other volcanic islands.

Zircons reveal magma fluxes in the Earth's crust.

Science.gov (United States)

Caricchi, Luca; Simpson, Guy; Schaltegger, Urs

2014-07-24

Magma fluxes regulate the planetary thermal budget, the growth of continents and the frequency and magnitude of volcanic eruptions, and play a part in the genesis and size of magmatic ore deposits. However, because a large fraction of the magma produced on the Earth does not erupt at the surface, determinations of magma fluxes are rare and this compromises our ability to establish a link between global heat transfer and large-scale geological processes. Here we show that age distributions of zircons, a mineral often present in crustal magmatic rocks, in combination with thermal modelling, provide an accurate means of retrieving magma fluxes. The characteristics of zircon age populations vary significantly and systematically as a function of the flux and total volume of magma accumulated in the Earth's crust. Our approach produces results that are consistent with independent determinations of magma fluxes and volumes of magmatic systems. Analysis of existing age population data sets using our method suggests that porphyry-type deposits, plutons and large eruptions each require magma input over different timescales at different characteristic average fluxes. We anticipate that more extensive and complete magma flux data sets will serve to clarify the control that the global heat flux exerts on the frequency of geological events such as volcanic eruptions, and to determine the main factors controlling the distribution of resources on our planet.

Redistribution of metabolic fluxes in Chlorella protothecoides by variation of media nitrogen concentration

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Saratram Gopalakrishnan

2015-12-01

Full Text Available In this study, the Elementary Metabolite Unit (EMU algorithm was employed to calculate intracellular fluxes for Chlorella protothecoides using previously generated growth and mass spec data. While the flux through glycolysis remained relatively constant, the pentose phosphate pathway (PPP flux increased from 3% to 20% of the glucose uptake during nitrogen-limited growth. The TCA cycle flux decreased from 94% to 38% during nitrogen-limited growth while the flux of acetyl-CoA into lipids increased from 58% to 109% of the glucose uptake, increasing total lipid accumulation. Phosphoenolpyruvate carboxylase (PEPCase activity was higher during nitrogen-sufficient growth. The glyoxylate shunt was found to be partially active in both cases, indicating the nutrient nature has an impact on flux distribution. It was found that the total NADPH supply within the cell remained almost constant under both conditions. In summary, algal cells substantially reorganize their metabolism during the switch from carbon-limited (nitrogen-sufficient to nitrogen-limited (carbon-sufficient growth. Keywords: Microalgae, Biofuels, Chlorella, MFA, EMU algorithm

Liquid and chemical fluxes in precipitation, throughfall and stemflow

DEFF Research Database (Denmark)

Pryor, S.C.; Barthelmie, R.J.

2005-01-01

Wet deposition (WD), throughfall (TF) and stemflow (SF) measurements undertaken in a deciduous forest show 85% of the WD liquid flux is observed as TF and approximately 2% as SF. TF and SF were observed to be enriched in base cations and accordingly had an average pH of 6.1 and 5.9, respectively...... composition below sugar maples. The total atmospheric flux of inorganic nitrogen to the forest is approximately 14-18 kg-N ha(-1) yr(-1) supercript stop with approximately half taken up by the canopy. Associated experiments designed to quantify uncertainties in the nutrient fluxes included laboratory tests...

Critical heat flux and post-critical heat flux performance of a 6-m, 37-element fully segmented bundle cooled by Freon-12

International Nuclear Information System (INIS)

Nickerson, J.R.

1982-05-01

A 6-m, 37-element, electrically heated bundle with full end plate simulation, cooled by Freon-12, has been tested for CHF (critical heat flux) and post-CHF conditions in the MR-3 Freon loop. The bundle was tested in a horizontal attitude and had a uniform axial heat flux distribution and radial heat flux depression. A total of 110 CHF points have been collected over the following range of water equivalent conditions: exit pressure 8.27 - 11.03 MPa, mass flux 1.38 - 8.14 Mg.m -2 .s -1 , inlet subcooling 0 - 500 kJ.kg -1 , outlet quality 10% - 37%. The data have been correlated on both a systems and local conditions basis over a limited mass flux range to within 2.8% rms. Significant CHF increases over smooth bundle results have been observed along with significant CHF improvement over a two end plate bundle simulation in the lower mass flux ranges. A satisfactory axial drypatch spreading correlation has been determined and extensive drypatch wall superheat mapping has been performed

Effects of mouthrinses with chlorhexidine and zinc ions combined with fluoride on the viability and glycolytic activity of dental plaque.

Science.gov (United States)

Giertsen, E; Scheie, A A

1995-10-01

Inhibition of plaque acidogenicity by a mouthrinse with chlorhexidine (CHX) or zinc ions has been ascribed to a prolonged bacteriostasis due to substantive properties of the agents. The present aim was to study the effects of mouthrinses with CHX and Zn ions combined with fluoride on the viability and glycolytic activity of dental plaque in order to assess the bacteriostatic versus possible bactericidal effects. Following 2 d of plaque accumulation, 4 groups of 10 students rinsed with either 12 mM NaF (F), 0.55 mM CHX diacetate+F (F-CHX), 10 mM Zn acetate+F (F-Zn), or with the three agents in combination (F-CHX-Zn). Plaque samples were collected before and 90 min after mouthrinsing. Thereafter, the in vivo plaque pH response to sucrose was monitored in each student using touch microelectrodes. F-CHX and F-CHX-Zn reduced the in vivo pH fall significantly as compared with F, whereas F-Zn exerted a non-significant inhibition. Pooled pre- and post-rinse plaque samples were used to measure the pH fall during fermentation of [14C]-glucose, and the glycolytic profiles were analyzed by HPLC. Bacterial viability was assessed by counting the colony-forming units (CFU). All mouthrinses except F reduced glucose consumption and acid formation and thus the pH fall. F-CHX reduced the CFU equal to the reduction of glucose consumption, indicating that inhibition of plaque acidogenicity was due to a bactericidal rather than a bacteriostatic effect. F and F-Zn did not reduce the CFU, thus F-Zn decreased glucose metabolism without affecting plaque viability. F-CHX-Zn reduced both the CFU and glucose metabolism of surviving plaque microorganisms.

An analytical transport theory method for calculating flux distribution in slab cells

International Nuclear Information System (INIS)

Abdel Krim, M.S.

2001-01-01

A transport theory method for calculating flux distributions in slab fuel cell is described. Two coupled integral equations for flux in fuel and moderator are obtained; assuming partial reflection at moderator external boundaries. Galerkin technique is used to solve these equations. Numerical results for average fluxes in fuel and moderator and the disadvantage factor are given. Comparison with exact numerical methods, that is for total reflection moderator outer boundaries, show that the Galerkin technique gives accurate results for the disadvantage factor and average fluxes. (orig.)

MCT1 modulates cancer cell pyruvate export and growth of tumors that co-express MCT1 and MCT4

Science.gov (United States)

Hong, Candice Sun; Graham, Nicholas A.; Gu, Wen; Camacho, Carolina Espindola; Mah, Vei; Maresh, Erin L.; Alavi, Mohammed; Bagryanova, Lora; Krotee, Pascal A. L.; Gardner, Brian K.; Behbahan, Iman Saramipoor; Horvath, Steve; Chia, David; Mellinghoff, Ingo K.; Hurvitz, Sara A.; Dubinett, Steven M.; Critchlow, Susan E.; Kurdistani, Siavash K.; Goodglick, Lee; Braas, Daniel; Graeber, Thomas G.; Christofk, Heather R.

2016-01-01

SUMMARY Monocarboxylate Transporter 1 (MCT1) inhibition is thought to block tumor growth through disruption of lactate transport and glycolysis. Here we show MCT1 inhibition impairs proliferation of glycolytic breast cancer cells co-expressing MCT1 and MCT4 via disruption of pyruvate rather than lactate export. MCT1 expression is elevated in glycolytic breast tumors, and high MCT1 expression predicts poor prognosis in breast and lung cancer patients. Acute MCT1 inhibition reduces pyruvate export but does not consistently alter lactate transport or glycolytic flux in breast cancer cells that co-express MCT1 and MCT4. Despite the lack of glycolysis impairment, MCT1 loss-of-function decreases breast cancer cell proliferation and blocks growth of mammary fat pad xenograft tumors. Our data suggest MCT1 expression is elevated in glycolytic cancers to promote pyruvate export, which when inhibited enhances oxidative metabolism and reduces proliferation. This study presents an alternative molecular consequence of MCT1 inhibitors further supporting their use as anti-cancer therapeutics. PMID:26876179

Fluxes of inorganic and organic arsenic species in a Norway spruce forest floor

Energy Technology Data Exchange (ETDEWEB)

Huang, J.-H. [Department of Soil Ecology, University of Bayreuth, D-95440 Bayreuth (Germany)], E-mail: jenhow.huang@uni-bayreuth.de; Matzner, Egbert [Department of Soil Ecology, University of Bayreuth, D-95440 Bayreuth (Germany)

2007-09-15

To identify the role of the forest floor in arsenic (As) biogeochemistry, concentrations and fluxes of inorganic and organic As in throughfall, litterfall and forest floor percolates at different layers were investigated. Nearly 40% of total As{sub total} input (5.3 g As ha{sup -1} yr{sup -1}) was retained in Oi layer, whereas As{sub total} fluxes from Oe and Oa layers exceeded the input by far (10.8 and 20 g As ha{sup -1} yr{sup -1}, respectively). Except dimethylarsinic acid (DMA), fluxes of organic As decreased with depth of forest floor so that <10% of total deposition (all <0.3 g As ha{sup -1} yr{sup -1}) reached the mineral soil. All forest floor layers are sinks for most organic As. Conversely, Oe and Oa layers are sources of As{sub total}, arsenite, arsenate and DMA. Significant correlations (r {>=} 0.43) between fluxes of As{sub total}, arsenite, arsenate or DMA and water indicate hydrological conditions and adsorption-desorption as factors influencing their release from the forest floor. The higher net release of arsenite from Oe and Oa and of DMA from Oa layer in the growing than dormant season also suggests microbial influences on the release of arsenite and DMA. - The forest floor layers are generally a source for inorganic arsenic species but a sink for most organic arsenic species under the present deposition rate.

Increased OXPHOS activity precedes rise in glycolytic rate in H-RasV12/E1A transformed fibroblasts that develop a Warburg phenotype

Directory of Open Access Journals (Sweden)

Pluk Helma

2009-07-01

Full Text Available Abstract Background The Warburg phenotype in cancer cells has been long recognized, but there is still limited insight in the consecutive metabolic alterations that characterize its establishment. We obtained better understanding of the coupling between metabolism and malignant transformation by studying mouse embryonic fibroblast-derived cells with loss-of-senescence or H-RasV12/E1A-transformed phenotypes at different stages of oncogenic progression. Results Spontaneous immortalization or induction of senescence-bypass had only marginal effects on metabolic profiles and viability. In contrast, H-RasV12/E1A transformation initially caused a steep increase in oxygen consumption and superoxide production, accompanied by massive cell death. During prolonged culture in vitro, cell growth rate increased gradually, along with tumor forming potential in in vitro anchorage-independent growth assays and in vivo tumor formation assays in immuno-deficient mice. Notably, glucose-to-lactic acid flux increased with passage number, while cellular oxygen consumption decreased. This conversion in metabolic properties was associated with a change in mitochondrial NAD+/NADH redox, indicative of decreased mitochondrial tricarboxic acid cycle and OXPHOS activity. Conclusion The high rate of oxidative metabolism in newly transformed cells is in marked contrast with the high glycolytic rate in cells in the later tumor stage. In our experimental system, with cells growing under ambient oxygen conditions in nutrient-rich media, the shift towards this Warburg phenotype occurred as a step-wise adaptation process associated with augmented tumorigenic capacity and improved survival characteristics of the transformed cells. We hypothesize that early-transformed cells, which potentially serve as founders for new tumor masses may escape therapies aimed at metabolic inhibition of tumors with a fully developed Warburg phenotype.

Evidence for Divergent Evolution of Growth Temperature Preference in Sympatric Saccharomyces Species

Science.gov (United States)

Gonçalves, Paula; Valério, Elisabete; Correia, Cláudia; de Almeida, João M. G. C. F.; Sampaio, José Paulo

2011-01-01

The genus Saccharomyces currently includes eight species in addition to the model yeast Saccharomyces cerevisiae, most of which can be consistently isolated from tree bark and soil. We recently found sympatric pairs of Saccharomyces species, composed of one cryotolerant and one thermotolerant species in oak bark samples of various geographic origins. In order to contribute to explain the occurrence in sympatry of Saccharomyces species, we screened Saccharomyces genomic data for protein divergence that might be correlated to distinct growth temperature preferences of the species, using the dN/dS ratio as a measure of protein evolution rates and pair-wise species comparisons. In addition to proteins previously implicated in growth at suboptimal temperatures, we found that glycolytic enzymes were among the proteins exhibiting higher than expected divergence when one cryotolerant and one thermotolerant species are compared. By measuring glycolytic fluxes and glycolytic enzymatic activities in different species and at different temperatures, we subsequently show that the unusual divergence of glycolytic genes may be related to divergent evolution of the glycolytic pathway aligning its performance to the growth temperature profiles of the different species. In general, our results support the view that growth temperature preference is a trait that may have undergone divergent selection in the course of ecological speciation in Saccharomyces. PMID:21674061

Recovery of aquatic insect-mediated methylmercury flux from ponds following drying disturbance.

Science.gov (United States)

Chumchal, Matthew M; Drenner, Ray W; Greenhill, Frank M; Kennedy, James H; Courville, Ashlyn E; Gober, Charlie A A; Lossau, Luke O

2017-08-01

Small ponds exist across a permanence gradient, and pond permanence is hypothesized to be a primary determinant of insect community structure and insect-mediated methylmercury (MeHg) flux from ponds to the surrounding terrestrial landscape. The present study describes the first experiment examining the recovery of insect-mediated MeHg flux following a drying disturbance that converted permanent ponds with insectivorous fish to semipermanent ponds without fish. Floating emergence traps were used to collect emergent insects for 10 wk in the spring and summer from 5 ponds with fish (permanent) and 5 ponds that were drained to remove fish, dried, and refilled with water (semipermanent). During the 73-d period after semipermanent ponds were refilled, total MeHg flux from semipermanent ponds was not significantly different than total MeHg flux from permanent ponds, indicating that insect-mediated MeHg flux had rapidly recovered in semipermanent ponds following the drying disturbance. Methylmercury fluxes from dragonflies (Odonata: Anisoptera) and phantom midges (Diptera: Chaoboridae) were significantly greater from newly refilled semipermanent ponds than permanent ponds, but the MeHg fluxes from the other 8 emergent insect taxa did not differ between treatments. The present study demonstrates the impact of drying disturbance and the effect of community structure on the cross-system transport of contaminants from aquatic to terrestrial ecosystems. Environ Toxicol Chem 2017;36:1986-1990. © 2017 SETAC. © 2017 SETAC.

High-frequency measurements of aeolian saltation flux: Field-based methodology and applications

Science.gov (United States)

Martin, Raleigh L.; Kok, Jasper F.; Hugenholtz, Chris H.; Barchyn, Thomas E.; Chamecki, Marcelo; Ellis, Jean T.

2018-02-01

Aeolian transport of sand and dust is driven by turbulent winds that fluctuate over a broad range of temporal and spatial scales. However, commonly used aeolian transport models do not explicitly account for such fluctuations, likely contributing to substantial discrepancies between models and measurements. Underlying this problem is the absence of accurate sand flux measurements at the short time scales at which wind speed fluctuates. Here, we draw on extensive field measurements of aeolian saltation to develop a methodology for generating high-frequency (up to 25 Hz) time series of total (vertically-integrated) saltation flux, namely by calibrating high-frequency (HF) particle counts to low-frequency (LF) flux measurements. The methodology follows four steps: (1) fit exponential curves to vertical profiles of saltation flux from LF saltation traps, (2) determine empirical calibration factors through comparison of LF exponential fits to HF number counts over concurrent time intervals, (3) apply these calibration factors to subsamples of the saltation count time series to obtain HF height-specific saltation fluxes, and (4) aggregate the calibrated HF height-specific saltation fluxes into estimates of total saltation fluxes. When coupled to high-frequency measurements of wind velocity, this methodology offers new opportunities for understanding how aeolian saltation dynamics respond to variability in driving winds over time scales from tens of milliseconds to days.

Neutrino fluxes produced by high energy solar flare particles

International Nuclear Information System (INIS)

Kolomeets, E.V.; Shmonin, V.L.

1975-01-01

In this work the calculated differential energy spectra of neutrinos poduced by high energy protons accelerated during 'small' solar flares are presented. The muon flux produced by neutrino interactions with the matter at large depths under the ground is calculated. The obtained flux of muons for the total number of solar flare accelerated protons of 10 28 - 10 32 is within 10 9 - 10 13 particles/cm 2 X s x ster. (orig.) [de

Flux Trapping Properties of Bulk HIGH-TC Superconductors in Static Field-Cooling Magnetization

Science.gov (United States)

Deng, Z.; Tsuzuki, K.; Miki, M.; Felder, B.; Hara, S.; Izumi, M.

2013-06-01

The trapping process and saturation effect of trapped magnetic flux of bulk high-temperature superconductors by static field-cooling magnetization (FCM) are reported in the paper. With a cryogenic Bell Hall sensor attached on the center of the bulk surface, the synchronous magnetic signals were recorded during the whole magnetization process. It enables us to know the flux trapping behavior since the removal of the excitation field, as well as the subsequent flux relaxation phenomenon and the flux dissipation in the quench process of the bulk sample. With the help of flux mapping techniques, the relationship between the trapped flux and the applied field was further investigated; the saturation effect of trapped flux was discussed by comparing the peak trapped field and total magnetic flux of the bulk sample. These studies are useful to understand the basic flux trapping properties of bulk superconductors.

Measurement of the Cosmic Ray and Neutrino-Induced Muon Flux at the Sudbury Neutrino Observatory

Science.gov (United States)

SNO collaboration; Aharmim, B.; Ahmed, S. N.; Andersen, T. C.; Anthony, A. E.; Barros, N.; Beier, E. W.; Bellerive, A.; Beltran, B.; Bergevin, M.; Biller, S. D.; Boudjemline, K.; Boulay, M. G.; Burritt, T. H.; Cai, B.; Chan, Y. D.; Chen, M.; Chon, M. C.; Cleveland, B. T.; Cox-Mobrand, G. A.; Currat, C. A.; Dai, X.; Dalnoki-Veress, F.; Deng, H.; Detwiler, J.; Doe, P. J.; Dosanjh, R. S.; Doucas, G.; Drouin, P.-L.; Duncan, F. A.; Dunford, M.; Elliott, S. R.; Evans, H. C.; Ewan, G. T.; Farine, J.; Fergani, H.; Fleurot, F.; Ford, R. J.; Formaggio, J. A.; Gagnon, N.; Goon, J. TM.; Grant, D. R.; Guillian, E.; Habib, S.; Hahn, R. L.; Hallin, A. L.; Hallman, E. D.; Hargrove, C. K.; Harvey, P. J.; Harvey, P. J.; Heeger, K. M.; Heintzelman, W. J.; Heise, J.; Helmer, R. L.; Hemingway, R. J.; Henning, R.; Hime, A.; Howard, C.; Howe, M. A.; Huang, M.; Jamieson, B.; Jelley, N. A.; Klein, J. R.; Kos, M.; Kruger, A.; Kraus, C.; Krauss, C. B.; Kutter, T.; Kyba, C. C. M.; Lange, R.; Law, J.; Lawson, I. T.; Lesko, K. T.; Leslie, J. R.; Levine, I.; Loach, J. C.; Luoma, S.; MacLellan, R.; Majerus, S.; Mak, H. B.; Maneira, J.; Marino, A. D.; Martin, R.; McCauley, N.; McDonald, A. B.; McGee, S.; Mifflin, C.; Miller, M. L.; Monreal, B.; Monroe, J.; Noble, A. J.; Oblath, N. S.; Okada, C. E.; O'Keeffe, H. M.; Opachich, Y.; Orebi Gann, G. D.; Oser, S. M.; Ott, R. A.; Peeters, S. J. M.; Poon, A. W. P.; Prior, G.; Rielage, K.; Robertson, B. C.; Robertson, R. G. H.; Rollin, E.; Schwendener, M. H.; Secrest, J. A.; Seibert, S. R.; Simard, O.; Simpson, J. J.; Sinclair, D.; Skensved, P.; Smith, M. W. E.; Sonley, T. J.; Steiger, T. D.; Stonehill, L. C.; Tagg, N.; Tesic, G.; Tolich, N.; Tsui, T.; Van de Water, R. G.; VanDevender, B. A.; Virtue, C. J.; Waller, D.; Waltham, C. E.; Wan Chan Tseung, H.; Wark, D. L.; Watson, P.; Wendland, J.; West, N.; Wilkerson, J. F.; Wilson, J. R.; Wouters, J. M.; Wright, A.; Yeh, M.; Zhang, F.; Zuber, K.

2009-07-10

Results are reported on the measurement of the atmospheric neutrino-induced muon flux at a depth of 2 kilometers below the Earth's surface from 1229 days of operation of the Sudbury Neutrino Observatory (SNO). By measuring the flux of through-going muons as a function of zenith angle, the SNO experiment can distinguish between the oscillated and un-oscillated portion of the neutrino flux. A total of 514 muon-like events are measured between -1 {le} cos {theta}{sub zenith} 0.4 in a total exposure of 2.30 x 10{sup 14} cm{sup 2} s. The measured flux normalization is 1.22 {+-} 0.09 times the Bartol three-dimensional flux prediction. This is the first measurement of the neutrino-induced flux where neutrino oscillations are minimized. The zenith distribution is consistent with previously measured atmospheric neutrino oscillation parameters. The cosmic ray muon flux at SNO with zenith angle cos {theta}{sub zenith} > 0.4 is measured to be (3.31 {+-} 0.01 (stat.) {+-} 0.09 (sys.)) x 10{sup -10} {micro}/s/cm{sup 2}.

Airborne particulate concentrations and fluxes at an active uranium mill tailings site

International Nuclear Information System (INIS)

Sehmel, G.A.

1978-01-01

Direct measurements of airborne particulate concentrations and fluxes of transported mill tailing materials were measured at an active mill tailings site. Experimental measurement equipment consisted of meteorological instrumentation to automatically activate total particulate air samplers as a function of wind speed increments and direction, as well as particle cascade impactors to measure airborne respirable concentrations as a function of particle size. In addition, an inertial impaction device measured nonrespirable fluxes of airborne particles. Caclulated results are presented in terms of the airborne solid concentration in g/m 3 , the horizontal airborne mass flux in g/(m 2 -day) for total collected nonrespirable particles and the radionuclide concentrations in dpm/g as a function of particle diameter for respirable and nonrespirable particles

Tests of a robust eddy correlation system for sensible heat flux

Science.gov (United States)

Blanford, J. H.; Gay, L. W.

1992-03-01

Sensible heat flux estimates from a simple, one-propeller eddy correlation system (OPEC) were compared with those from a sonic anemometer eddy correlation system (SEC). In accordance with similarity theory, the performance of the OPEC system improved with increasing height of the sensor above the surface. Flux totals from the two systems at sites with adequate fetch were in excellent agreement after frequency response corrections were applied. The propeller system appears suitable for long periods of unattended measurement. The sensible heat flux measurements can be combined with net radiation and soil heat flux measurements to estimate latent heat as a residual in the surface energy balance.

Ketone bodies effectively compete with glucose for neuronal acetyl-CoA generation in rat hippocampal slices.

Science.gov (United States)

Valente-Silva, Paula; Lemos, Cristina; Köfalvi, Attila; Cunha, Rodrigo A; Jones, John G

2015-09-01

Ketone bodies can be used for cerebral energy generation in situ, when their availability is increased as during fasting or ingestion of a ketogenic diet. However, it is not known how effectively ketone bodies compete with glucose, lactate, and pyruvate for energy generation in the brain parenchyma. Hence, the contributions of exogenous 5.0 mM [1-(13)C]glucose and 1.0 mM [2-(13)C]lactate + 0.1 mM pyruvate (combined [2-(13)C]lactate + [2-(13)C]pyruvate) to acetyl-CoA production were measured both without and with 5.0 mM [U-(13)C]3-hydroxybutyrate in superfused rat hippocampal slices by (13)C NMR non-steady-state isotopomer analysis of tissue glutamate and GABA. Without [U-(13)C]3-hydroxybutyrate, glucose, combined lactate + pyruvate, and unlabeled endogenous sources contributed (mean ± SEM) 70 ± 7%, 10 ± 2%, and 20 ± 8% of acetyl-CoA, respectively. With [U-(13)C]3-hydroxybutyrate, glucose contributions significantly fell from 70 ± 7% to 21 ± 3% (p neurons. The appearance of superfusate lactate derived from glycolysis of [1-(13)C]glucose did not decrease significantly in the presence of 3-hydroxybutyrate, hence total glycolytic flux (Krebs cycle inflow + exogenous lactate formation) was attenuated by 3-hydroxybutyrate. This indicates that, under these conditions, 3-hydroxybutyrate inhibited glycolytic flux upstream of pyruvate kinase. Copyright © 2015 John Wiley & Sons, Ltd.

Adaptation to the coupling of glycolysis to toxic methylglyoxal production in tpiA deletion strains of Escherichia coli requires synchronized and counterintuitive genetic changes

DEFF Research Database (Denmark)

McCloskey, Douglas; Xu, Sibei; Sandberg, Troy E.

2018-01-01

-talk between carbon starvation and inorganic phosphate signalling was revealed in the tpiA deletion strain that required mutations in inorganic phosphate signalling mechanisms to alleviate; and 3) The split flux through lower glycolysis depleted glycolytic intermediates requiring a host of synchronized...

Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney

OpenAIRE

Ishitsuka, Yoichi; Fukumoto, Yusuke; Kondo, Yuki; Irikura, Mitsuru; Kadowaki, Daisuke; Narita, Yuki; Hirata, Sumio; Moriuchi, Hiroshi; Maruyama, Toru; Hamasaki, Naotaka; Irie, Tetsumi

2013-01-01

We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thi...

From elementary flux modes to elementary flux vectors: Metabolic pathway analysis with arbitrary linear flux constraints

Science.gov (United States)

Klamt, Steffen; Gerstl, Matthias P.; Jungreuthmayer, Christian; Mahadevan, Radhakrishnan; Müller, Stefan

2017-01-01

Elementary flux modes (EFMs) emerged as a formal concept to describe metabolic pathways and have become an established tool for constraint-based modeling and metabolic network analysis. EFMs are characteristic (support-minimal) vectors of the flux cone that contains all feasible steady-state flux vectors of a given metabolic network. EFMs account for (homogeneous) linear constraints arising from reaction irreversibilities and the assumption of steady state; however, other (inhomogeneous) linear constraints, such as minimal and maximal reaction rates frequently used by other constraint-based techniques (such as flux balance analysis [FBA]), cannot be directly integrated. These additional constraints further restrict the space of feasible flux vectors and turn the flux cone into a general flux polyhedron in which the concept of EFMs is not directly applicable anymore. For this reason, there has been a conceptual gap between EFM-based (pathway) analysis methods and linear optimization (FBA) techniques, as they operate on different geometric objects. One approach to overcome these limitations was proposed ten years ago and is based on the concept of elementary flux vectors (EFVs). Only recently has the community started to recognize the potential of EFVs for metabolic network analysis. In fact, EFVs exactly represent the conceptual development required to generalize the idea of EFMs from flux cones to flux polyhedra. This work aims to present a concise theoretical and practical introduction to EFVs that is accessible to a broad audience. We highlight the close relationship between EFMs and EFVs and demonstrate that almost all applications of EFMs (in flux cones) are possible for EFVs (in flux polyhedra) as well. In fact, certain properties can only be studied with EFVs. Thus, we conclude that EFVs provide a powerful and unifying framework for constraint-based modeling of metabolic networks. PMID:28406903

Intermittency route to chaos in a biochemical system.

Science.gov (United States)

De la Fuente, I M; Martinez, L; Veguillas, J

1996-01-01

The numerical analysis of a glycolytic model performed through the construction of a system of three differential-delay equations reveals a phenomenon of intermittency route to chaos. In our biochemical system, the consideration of delay time variations under constant input flux as well as frequency variations of the periodic substrate input flux allows us, in both cases, to observe a type of transition to chaos different from the 'Feigenbaum route'.

Gap-related trapped magnetic flux dependence between single and combined bulk superconductors

Science.gov (United States)

Deng, Z.; Miki, M.; Felder, B.; Tsuzuki, K.; Shinohara, N.; Uetake, T.; Izumi, M.

2011-05-01

Aiming at examining the trapped-flux dependence between single and combined bulk superconductors for field-pole applications, three rectangular Y 1.65Ba 2Cu 3O 7-x (YBCO) bulks with a possibly compact combination were employed to investigate the trapped-flux characteristics of single and combined bulks with a field-cooling magnetization (FCM) method. A gap-related dependence was found between them. At lower gaps of 1 mm and 5 mm, the peak trapped fields and total magnetic flux of combined bulks are both smaller than the additive values of each single bulk, which can be ascribed to the demagnetization influences of the field around the bulk generated by the adjacent ones. While, at larger gaps like 10 mm, the situation becomes reversed. The combined bulks can attain bigger peak trapped fields as well as total magnetic flux, which indicates that the magnetic field by the bulk combination can reach higher gaps, thanks to the bigger magnetic energy compared with the single bulk. The presented results show that, on one hand, it is possible to estimate the total trapped magnetic flux of combined bulks by an approximate additive method of each single bulk while considering a demagnetization factor; on the other hand, it also means that the performance of combined bulks will be superior to the addition of each single bulk at larger gaps, thus preferable for large-scaled magnet applications.

Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis

DEFF Research Database (Denmark)

Købmann, Brian Jensen; Solem, Christian; Jensen, Peter Ruhdal

2005-01-01

control on glycolysis and growth rate but high negative control on formate production. We find that PFK and PK have zero control on glycolysis and growth rate at the wildtype enzyme level but both enzymes exert strong positive control on the glycolytic flux at reduced activities. PK has high positive...... coefficient increased towards 3. Increased las expression resulted in a slight decrease in the glycolytic flux. At the wildtype level the control was close to zero on both glycolysis and the pyruvate branches. The sum of control coefficients for the three enzymes individually was comparable to the control...... coefficient found for the entire operon; the strong positive control by PK almost cancels out the negative control by LDH on formate production. The analysis suggests that co-regulation of PFK and PK provides a very efficient way to regulate glycolysis, and co-regulating PK and LDH allows the cells...

Diffusive flux of methane from warm wetlands

Energy Technology Data Exchange (ETDEWEB)

Barber, T.R.; Burke, R.A.; Sackett, W.M. (Univ. of South Florida, St. Petersburg (USA))

1988-12-01

Diffusion of methane across the air-water interface from several wetland environments in south Florida was estimated from measured surface water concentrations using an empirically derived gas exchange model. The flux from the Everglades sawgrass marsh system varied widely, ranging from 0.18 + or{minus}0.21 mol CH{sub 4}/sq m/yr for densely vegetated regions to 2.01 + or{minus}0.88 for sparsely vegetated, calcitic mud areas. Despite brackish salinities, a strong methane flux, 1.87 + or{minus}0.63 mol CH{sub 4}/sq m/yr, was estimated for an organic-rich mangrove pond near Florida Bay. The diffusive flux accounted for 23, 36, and 13% of the total amount of CH{sub 4} emitted to the atmosphere from these environments, respectively. The average dissolved methane concentration for an organic-rich forested swamp was the highest of any site at 12.6 microM; however, the calculated diffusive flux from this location, 2.57 + or{minus}1.88 mol CH{sub 4}/sq m/yr, was diminished by an extensive plant canopy that sheltered the air-water interface from the wind. The mean diffusive flux from four freshwater lakes, 0.77 + or{minus}0.73 mol CH{sub 4}/sq m/yr, demonstrated little temperature dependence. The mean diffusive flux for an urbanized, subtropical estuary was 0.06 + or{minus}0.05 mol CH{sub 4}/sq m/yr.

FluxVisualizer, a Software to Visualize Fluxes through Metabolic Networks

Directory of Open Access Journals (Sweden)

Tim Daniel Rose

2018-04-01

Full Text Available FluxVisualizer (Version 1.0, 2017, freely available at https://fluxvisualizer.ibgc.cnrs.fr is a software to visualize fluxes values on a scalable vector graphic (SVG representation of a metabolic network by colouring or increasing the width of reaction arrows of the SVG file. FluxVisualizer does not aim to draw metabolic networks but to use a customer’s SVG file allowing him to exploit his representation standards with a minimum of constraints. FluxVisualizer is especially suitable for small to medium size metabolic networks, where a visual representation of the fluxes makes sense. The flux distribution can either be an elementary flux mode (EFM, a flux balance analysis (FBA result or any other flux distribution. It allows the automatic visualization of a series of pathways of the same network as is needed for a set of EFMs. The software is coded in python3 and provides a graphical user interface (GUI and an application programming interface (API. All functionalities of the program can be used from the API and the GUI and allows advanced users to add their own functionalities. The software is able to work with various formats of flux distributions (Metatool, CellNetAnalyzer, COPASI and FAME export files as well as with Excel files. This simple software can save a lot of time when evaluating fluxes simulations on a metabolic network.

Modelling stomatal ozone flux and deposition to grassland communities across Europe

International Nuclear Information System (INIS)

Ashmore, M.R.; Bueker, P.; Emberson, L.D.; Terry, A.C.; Toet, S.

2007-01-01

Regional scale modelling of both ozone deposition and the risk of ozone impacts is poorly developed for grassland communities. This paper presents new predictions of stomatal ozone flux to grasslands at five different locations in Europe, using a mechanistic model of canopy development for productive grasslands to generate time series of leaf area index and soil water potential as inputs to the stomatal component of the DO 3 SE ozone deposition model. The parameterisation of both models was based on Lolium perenne, a dominant species of productive pasture in Europe. The modelled seasonal time course of stomatal ozone flux to both the whole canopy and to upper leaves showed large differences between climatic zones, which depended on the timing of the start of the growing season, the effect of soil water potential, and the frequency of hay cuts. Values of modelled accumulated flux indices and the AOT40 index showed a five-fold difference between locations, but the locations with the highest flux differed depending on the index used; the period contributing to the accumulation of AOT40 did not always coincide with the modelled period of active ozone canopy uptake. Use of a fixed seasonal profile of leaf area index in the flux model produced very different estimates of annual accumulated total canopy and leaf ozone flux when compared with the flux model linked to a simulation of canopy growth. Regional scale model estimates of both the risks of ozone impacts and of total ozone deposition will be inaccurate unless the effects of climate and management in modifying grass canopy growth are incorporated. - Modelled stomatal flux of ozone to productive grasslands in Europe shows different spatial and temporal variation to AOT40, and is modified by management and soil water status

Convective and large-scale mass flux profiles over tropical oceans determined from synergistic analysis of a suite of satellite observations

Science.gov (United States)

Masunaga, Hirohiko; Luo, Zhengzhao Johnny

2016-07-01

A new, satellite-based methodology is developed to evaluate convective mass flux and large-scale total mass flux. To derive the convective mass flux, candidate profiles of in-cloud vertical velocity are first constructed with a simple plume model under the constraint of ambient sounding and then narrowed down to the solution that matches satellite-derived cloud top buoyancy. Meanwhile, the large-scale total mass flux is provided separately from satellite soundings by a method developed previously. All satellite snapshots are sorted into a composite time series that delineates the evolution of a vigorous and organized convective system. Principal findings are the following. First, convective mass flux is modulated primarily by convective cloud cover, with the intensity of individual convection being less variable over time. Second, convective mass flux dominates the total mass flux only during the early hours of the convective evolution; as convective system matures, a residual mass flux builds up in the mass flux balance that is reminiscent of stratiform dynamics. The method developed in this study is expected to be of unique utility for future observational diagnosis of tropical convective dynamics and for evaluation of global climate model cumulus parameterizations in a global sense.

Combining sap flow and eddy covariance approaches to derive stomatal and non-stomatal O3 fluxes in a forest stand

International Nuclear Information System (INIS)

Nunn, A.J.; Cieslik, S.; Metzger, U.; Wieser, G.; Matyssek, R.

2010-01-01

Stomatal O 3 fluxes to a mixed beech/spruce stand (Fagus sylvatica/Picea abies) in Central Europe were determined using two different approaches. The sap flow technique yielded the tree-level transpiration, whereas the eddy covariance method provided the stand-level evapotranspiration. Both data were then converted into stomatal ozone fluxes, exemplifying this novel concept for July 2007. Sap flow-based stomatal O 3 flux was 33% of the total O 3 flux, whereas derivation from evapotranspiration rates in combination with the Penman-Monteith algorithm amounted to 47%. In addition to this proportional difference, the sap flow-based assessment yielded lower levels of stomatal O 3 flux and reflected stomatal regulation rather than O 3 exposure, paralleling the daily courses of canopy conductance for water vapor and eddy covariance-based total stand-level O 3 flux. The demonstrated combination of sap flow and eddy covariance approaches supports the development of O 3 risk assessment in forests from O 3 exposure towards flux-based concepts. - Combined tree sap flow and eddy covariance-based methodologies yield stomatal O 3 flux as 33% in total stand flux.

Planck intermediate results - LII. Planet flux densities

DEFF Research Database (Denmark)

Akrami, Y.; Ashdown, M.; Aumont, J.

2017-01-01

Measurements of flux density are described for five planets, Mars, Jupiter, Saturn, Uranus, and Neptune, across the six Planck High Frequency Instrument frequency bands (100–857 GHz) and these are then compared with models and existing data. In our analysis, we have also included estimates...... of the brightness of Jupiter and Saturn at the three frequencies of the Planck Low Frequency Instrument (30, 44, and 70 GHz). The results provide constraints on the intrinsic brightness and the brightness time-variability of these planets. The majority of the planet flux density estimates are limited by systematic...... errors, but still yield better than 1% measurements in many cases. Applying data from Planck HFI, the Wilkinson Microwave Anisotropy Probe (WMAP), and the Atacama Cosmology Telescope (ACT) to a model that incorporates contributions from Saturn’s rings to the planet’s total flux density suggests a best...

Hydrocarbon and Carbon Dioxide Fluxes from Natural Gas Well Pad Soils and Surrounding Soils in Eastern Utah.

Science.gov (United States)

Lyman, Seth N; Watkins, Cody; Jones, Colleen P; Mansfield, Marc L; McKinley, Michael; Kenney, Donna; Evans, Jordan

2017-10-17

We measured fluxes of methane, nonmethane hydrocarbons, and carbon dioxide from natural gas well pad soils and from nearby undisturbed soils in eastern Utah. Methane fluxes varied from less than zero to more than 38 g m -2 h -1 . Fluxes from well pad soils were almost always greater than from undisturbed soils. Fluxes were greater from locations with higher concentrations of total combustible gas in soil and were inversely correlated with distance from well heads. Several lines of evidence show that the majority of emission fluxes (about 70%) were primarily due to subsurface sources of raw gas that migrated to the atmosphere, with the remainder likely caused primarily by re-emission of spilled liquid hydrocarbons. Total hydrocarbon fluxes during summer were only 39 (16, 97)% as high as during winter, likely because soil bacteria consumed the majority of hydrocarbons during summer months. We estimate that natural gas well pad soils account for 4.6 × 10 -4 (1.6 × 10 -4 , 1.6 × 10 -3 )% of total emissions of hydrocarbons from the oil and gas industry in Utah's Uinta Basin. Our undisturbed soil flux measurements were not adequate to quantify rates of natural hydrocarbon seepage in the Uinta Basin.

Numerical simulation of heat fluxes in a two-temperature plasma at shock tube walls

International Nuclear Information System (INIS)

Kuznetsov, E A; Poniaev, S A

2015-01-01

Numerical simulation of a two-temperature three-component Xenon plasma flow is presented. A solver based on the OpenFOAM CFD software package is developed. The heat flux at the shock tube end wall is calculated and compared with experimental data. It is shown that the heat flux due to electrons can be as high as 14% of the total heat flux. (paper)

Numerical simulation of heat fluxes in a two-temperature plasma at shock tube walls

Science.gov (United States)

Kuznetsov, E. A.; Poniaev, S. A.

2015-12-01

Numerical simulation of a two-temperature three-component Xenon plasma flow is presented. A solver based on the OpenFOAM CFD software package is developed. The heat flux at the shock tube end wall is calculated and compared with experimental data. It is shown that the heat flux due to electrons can be as high as 14% of the total heat flux.

Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites.

OpenAIRE

Santangelo, G M; Tornow, J

1990-01-01

Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity.

What controls glycolysis in bloodstream form Trypanosoma brucei?

NARCIS (Netherlands)

Bakker, B.M.; Michels, P.A.M.; Opperdoes, F.R.; Westerhoff, H.V.

1999-01-01

On the basis of the experimentally determined kinetic properties of the trypanosomal enzymes, the question is addressed of which step limits the glycolytic flux in bloodstream form Trypanosoma brucei. There appeared to be no single answer; in the physiological range, control shifted between the

Coronal Flux Rope Catastrophe Associated With Internal Energy Release

Science.gov (United States)

Zhuang, Bin; Hu, Youqiu; Wang, Yuming; Zhang, Quanhao; Liu, Rui; Gou, Tingyu; Shen, Chenglong

2018-04-01

Magnetic energy during the catastrophe was predominantly studied by the previous catastrophe works since it is believed to be the main energy supplier for the solar eruptions. However, the contribution of other types of energies during the catastrophe cannot be neglected. This paper studies the catastrophe of the coronal flux rope system in the solar wind background, with emphasis on the transformation of different types of energies during the catastrophe. The coronal flux rope is characterized by its axial and poloidal magnetic fluxes and total mass. It is shown that a catastrophe can be triggered by not only an increase but also a decrease of the axial magnetic flux. Moreover, the internal energy of the rope is found to be released during the catastrophe so as to provide energy for the upward eruption of the flux rope. As far as the magnetic energy is concerned, it provides only part of the energy release, or even increases during the catastrophe, so the internal energy may act as the dominant or even the unique energy supplier during the catastrophe.

Ethanologenic Enzymes of Zymomonas mobilis

Energy Technology Data Exchange (ETDEWEB)

Ingram, Lonnie O' Neal

1999-03-01

Zymomonas mobilis is a unique microorganism in being both obligately fermentative and utilizing a Entner-Doudoroff pathway for glycolysis. Glycolytic flux in this organism is readily measured as evolved carbon dioxide, ethanol, or glucose consumed and exceeds 1 {micro}mole glucose/min per mg cell protein. To support this rapid glycolysis, approximately 50% of cytoplasmic protein is devoted to the 13 glycolytic and fermentative enzymes which constitute this central catabolic pathway. Only 1 ATP (net) is produced from each glucose metabolized. During the past grant period, we have completed the characterization of 11 of the 13 glycolytic genes from Z. mobilis together with complementary but separate DOE-fimded research by a former post-dot and collaborator, Dr. Tyrrell Conway. Research funded in my lab by DOE, Division of Energy Biosciences can be divided into three sections: A. Fundamental studies; B. Applied studies and utility; and C. Miscellaneous investigations.

Measuring Convective Mass Fluxes Over Tropical Oceans

Science.gov (United States)

Raymond, David

2017-04-01

Deep convection forms the upward branches of all large-scale circulations in the tropics. Understanding what controls the form and intensity of vertical convective mass fluxes is thus key to understanding tropical weather and climate. These mass fluxes and the corresponding conditions supporting them have been measured by recent field programs (TPARC/TCS08, PREDICT, HS3) in tropical disturbances considered to be possible tropical storm precursors. In reality, this encompasses most strong convection in the tropics. The measurements were made with arrays of dropsondes deployed from high altitude. In some cases Doppler radar provided additional measurements. The results are in some ways surprising. Three factors were found to control the mass flux profiles, the strength of total surface heat fluxes, the column-integrated relative humidity, and the low to mid-tropospheric moist convective instability. The first two act as expected, with larger heat fluxes and higher humidity producing more precipitation and stronger lower tropospheric mass fluxes. However, unexpectedly, smaller (but still positive) convective instability produces more precipitation as well as more bottom-heavy convective mass flux profiles. Furthermore, the column humidity and the convective instability are anti-correlated, at least in the presence of strong convection. On spatial scales of a few hundred kilometers, the virtual temperature structure appears to be in dynamic balance with the pattern of potential vorticity. Since potential vorticity typically evolves on longer time scales than convection, the potential vorticity pattern plus the surface heat fluxes then become the immediate controlling factors for average convective properties. All measurements so far have taken place in regions with relatively flat sea surface temperature (SST) distributions. We are currently seeking funding for a measurement program in the tropical east Pacific, a region that exhibits strong SST gradients and

River inputs and organic matter fluxes in the northern Bay of Bengal: Fatty acids

Digital Repository Service at National Institute of Oceanography (India)

Reemtsma, T.; Ittekkot, V.; Bartsch, M.; Nair, R.R

) 55-71 55 Elsevier Science Publishers B.V., Amsterdam \\[RA\\] River inputs and organic matter fluxes in the northern Bay of Bengal: fatty acids T. Reemtsma a, V. Ittekkot a, M. Bartsch a and R.R. Nair b alnstitut fiir Biogeochemie und Meereschemie..., R.R., 1993. River inputs and organic matter fluxes in the northern Bay of Bengal: fatty acids. Chem. Geol., 103: 55-71. Total particulate matter flux and organic carbon and fatty acid fluxes associated with settling particles collected during...

Flux recovery of ceramic tubular membranes fouled with whey proteins: Some aspects of membrane cleaning

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Popović Svetlana S.

2008-01-01

Full Text Available Efficiency of membrane processes is greatly affected by the flux reduction due to the deposits formation at the surface and/or in the pores of the membrane. Efficiency of membrane processes is affected by cleaning procedure applied to regenerate flux. In this work, flux recovery of ceramic tubular membranes with 50 and 200 nm pore size was investigated. The membranes were fouled with reconstituted whey solution for 1 hour. After that, the membranes were rinsed with clean water and then cleaned with sodium hydroxide solutions or formulated detergents (combination of P3 Ultrasil 67 and P3 Ultrasil 69. Flux recovery after the rinsing step was not satisfactory although fouling resistance reduction was significant so that chemical cleaning was necessary. In the case of 50 nm membrane total flux recovery was achieved after cleaning with 1.0% (w/w sodium hydroxide solution. In the case of 200 nm membrane total flux recovery was not achieved irrespective of the cleaning agent choice and concentration. Cleaning with commercial detergent was less efficient than cleaning with the sodium hydroxide solution.

Land-use effects on fluxes of suspended sediment, nitrogen and phosphorus from a river catchment of the Great Barrier Reef, Australia

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Hunter, Heather M.; Walton, Richard S.

2008-07-01

SummaryA 6-year study was conducted in the Johnstone River system in the wet tropics of north-eastern Australia, to address concerns that the Great Barrier Reef is at risk from elevated levels of suspended sediment (SS) and nutrients discharged from its river catchments. Aims were to quantify: (i) fluxes of SS, phosphorus (P) and nitrogen (N) exported annually from the catchment and (ii) the influence of rural land uses on these fluxes. Around 55% of the 1602 km2 catchment was native rainforest, with the reminder developed mainly for livestock and crop production. Water quality and stream flow were monitored at 16 sites, with the emphasis on sampling major runoff events. Monitoring data were used to calibrate a water quality model for the catchment (HSPF), which was run with 39 years of historical precipitation and evaporation data. Modelled specific fluxes from the catchment of 1.2 ± 1.1 t SS ha-1 y-1, 2.2 ± 1.8 kg P ha-1 y-1 and 11.4 ± 7.3 kg N ha-1y-1 were highly variable between and within years. Fluxes of SS and P were strongly dominated by major events, with 91% of SS and 84% of P exported during the highest 10% of daily flows. On average, sediment P comprised 81% of the total P flux. The N flux was less strongly dominated by major events and sediment N comprised 46% of total N exports. Specific fluxes of SS, N and P from areas receiving precipitation of 3545 mm y-1 were around 3-4 times those from areas receiving 1673 mm y-1. For a given mean annual precipitation, specific fluxes of SS and P from beef pastures, dairy pastures and unsewered residential areas were similar to those from rainforest, while fluxes from areas of sugar cane and bananas were 3-4 times higher. Specific fluxes of N from areas with an annual precipitation of 3545 mm ranged from 8.9 ± 6.5 kg N ha-1 y-1 (rainforest) to 72 ± 50 kg N ha-1 y-1 (unsewered residential). Aggregated across the entire catchment, disproportionately large fluxes of SS, total P and total N were derived from

Neutron energy spectrum flux profile of Ghana's miniature neutron source reactor core

International Nuclear Information System (INIS)

Sogbadji, R.B.M.; Abrefah, R.G.; Ampomah-Amoako, E.; Agbemava, S.E.; Nyarko, B.J.B.

2011-01-01

Highlights: → The total neutron flux spectrum of the compact core of Ghana's miniature neutron source reactor was studied. → Using 20,484 energy grids, the thermal, slowing down and fast neutron energy regions were studied. - Abstract: The total neutron flux spectrum of the compact core of Ghana's miniature neutron source reactor was understudied using the Monte Carlo method. To create small energy groups, 20,484 energy grids were used for the three neutron energy regions: thermal, slowing down and fast. The moderator, the inner irradiation channels, the annulus beryllium reflector and the outer irradiation channels were the region monitored. The thermal neutrons recorded their highest flux in the inner irradiation channel with a peak flux of (1.2068 ± 0.0008) x 10 12 n/cm 2 s, followed by the outer irradiation channel with a peak flux of (7.9166 ± 0.0055) x 10 11 n/cm 2 s. The beryllium reflector recorded the lowest flux in the thermal region with a peak flux of (2.3288 ± 0.0004) x 10 11 n/cm 2 s. The peak values of the thermal energy range occurred in the energy range (1.8939-3.7880) x 10 -08 MeV. The inner channel again recorded the highest flux of (1.8745 ± 0.0306) x 10 09 n/cm 2 s at the lower energy end of the slowing down region between 8.2491 x 10 -01 MeV and 8.2680 x 10 -01 MeV, but was over taken by the moderator as the neutron energies increased to 2.0465 MeV. The outer irradiation channel recorded the lowest flux in this region. In the fast region, the core, where the moderator is found, the highest flux was recorded as expected, at a peak flux of (2.9110 ± 0.0198) x 10 08 n/cm 2 s at 6.961 MeV. The inner channel recorded the second highest while the outer channel and annulus beryllium recorded very low flux in this region. The flux values in this region reduce asymptotically to 20 MeV.

SPACETRAN, Radiation Leakage from Cylinder with ANISN Flux Calculation

International Nuclear Information System (INIS)

Cramer, S.N.; Solomito, M.

1974-01-01

1 - Nature of physical problem solved: SPACETRAN is designed to calculate the energy-dependent total flux or some proportional quantity such as kerma, due to the radiation leakage from the surface of a right-circular cylinder at detector positions located at arbitrary distances from the surface. The assumptions are made that the radiation emerging from the finite cylinder has no spatial dependence and that a vacuum surrounds the cylinder. 2 - Method of solution: There are three versions of the program in the code package. SPACETRAN-I uses the surface angular fluxes calculated by the discrete ordinates SN code ANISN, as input. SPACETRAN-II assumes that the surface angular flux for all energies can be represented as a function (Cos(PHI))**N, where PHI is the angle between surface outward normal and radiation direction, and N is an integer specified by the user. For both versions the energy group structure and the number and location of detectors is arbitrary. The flux (or response function) for a given energy group at some detection point is computed by summing the contributions from each surface area element over the entire surface. The surface area elements are defined by input data. SPACETRAN-III uses surface angular fluxes from DOT-3. SPACETRAN-I handles contributions either from a cylinder 'end' or 'side', so the total contributions must be obtained by adding the results of separate end and side runs. ANISN angular fluxes are specified for discrete directions. In general, the direction between the detector and contributing area will not exactly coincide with one of these discrete directions. In this case, the ANISN angular flux for the 'closest' discrete direction is used to approximate the contribution to the detector. SPACETRAN-II handles contributions from both the side and end of a cylinder in a single run. Since the assumed angular distribution is specified by a continuous function, it is not necessary to perform the angle selection described above. For

CO2 flux from Javanese mud volcanism.

Science.gov (United States)

Queißer, M; Burton, M R; Arzilli, F; Chiarugi, A; Marliyani, G I; Anggara, F; Harijoko, A

2017-06-01

Studying the quantity and origin of CO 2 emitted by back-arc mud volcanoes is critical to correctly model fluid-dynamical, thermodynamical, and geochemical processes that drive their activity and to constrain their role in the global geochemical carbon cycle. We measured CO 2 fluxes of the Bledug Kuwu mud volcano on the Kendeng Fold and thrust belt in the back arc of Central Java, Indonesia, using scanning remote sensing absorption spectroscopy. The data show that the expelled gas is rich in CO 2 with a volume fraction of at least 16 vol %. A lower limit CO 2 flux of 1.4 kg s -1 (117 t d -1 ) was determined, in line with the CO 2 flux from the Javanese mud volcano LUSI. Extrapolating these results to mud volcanism from the whole of Java suggests an order of magnitude total CO 2 flux of 3 kt d -1 , comparable with the expected back-arc efflux of magmatic CO 2 . After discussing geochemical, geological, and geophysical evidence we conclude that the source of CO 2 observed at Bledug Kuwu is likely a mixture of thermogenic, biogenic, and magmatic CO 2 , with faulting controlling potential pathways for magmatic fluids. This study further demonstrates the merit of man-portable active remote sensing instruments for probing natural gas releases, enabling bottom-up quantification of CO 2 fluxes.

Apparatus and method for quantitatively evaluating total fissile and total fertile nuclide content in samples

International Nuclear Information System (INIS)

Caldwell, J.T.; Cates, M.R.; Franks, L.A.; Kunz, W.E.

1985-01-01

Simultaneous photon and neutron interrogation of samples for the quantitative determination of total fissile nuclide and total fertile nuclide material present is made possible by the use of an electron accelerator. Prompt and delayed neutrons produced from resulting induced fissions are counted using a single detection system and allow the resolution of the contributions from each interrogating flux leading in turn to the quantitative determination sought. Detection limits for 239 Pu are estimated to be about 3 mg using prompt fission neutrons and about 6 mg using delayed delayed neutrons

Quantifying the drivers of ocean-atmosphere CO2 fluxes

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Lauderdale, Jonathan M.; Dutkiewicz, Stephanie; Williams, Richard G.; Follows, Michael J.

2016-07-01

A mechanistic framework for quantitatively mapping the regional drivers of air-sea CO2 fluxes at a global scale is developed. The framework evaluates the interplay between (1) surface heat and freshwater fluxes that influence the potential saturated carbon concentration, which depends on changes in sea surface temperature, salinity and alkalinity, (2) a residual, disequilibrium flux influenced by upwelling and entrainment of remineralized carbon- and nutrient-rich waters from the ocean interior, as well as rapid subduction of surface waters, (3) carbon uptake and export by biological activity as both soft tissue and carbonate, and (4) the effect on surface carbon concentrations due to freshwater precipitation or evaporation. In a steady state simulation of a coarse-resolution ocean circulation and biogeochemistry model, the sum of the individually determined components is close to the known total flux of the simulation. The leading order balance, identified in different dynamical regimes, is between the CO2 fluxes driven by surface heat fluxes and a combination of biologically driven carbon uptake and disequilibrium-driven carbon outgassing. The framework is still able to reconstruct simulated fluxes when evaluated using monthly averaged data and takes a form that can be applied consistently in models of different complexity and observations of the ocean. In this way, the framework may reveal differences in the balance of drivers acting across an ensemble of climate model simulations or be applied to an analysis and interpretation of the observed, real-world air-sea flux of CO2.

The impact of lateral carbon fluxes on the European carbon balance

International Nuclear Information System (INIS)

Ciais, P.; Hauglustaine, D.; Borges, A.V.; Abril, G.; Meybeck, M.; Folberth, G.; Janssens, I.A.

2008-01-01

To date, little is known about the impact of processes which cause lateral carbon fluxes over continents, and from continents to oceans on the CO 2 - and carbon budgets at local, regional and continental scales. Lateral carbon fluxes contribute to regional carbon budgets as follows: Ecosystem CO 2 sink=Ecosystem carbon accumulation + Lateral carbon fluxes. We estimated the contribution of wood and food product trade, of emission and oxidation of reduced carbon species, and of river erosion and transport as lateral carbon fluxes to the carbon balance of Europe (EU-25). The analysis is completed by new estimates of the carbon fluxes of coastal seas. We estimated that lateral transport (all processes combined) is a flux of 165 Tg C yr -1 at the scale of EU-25. The magnitude of lateral transport is thus comparable to current estimates of carbon accumulation in European forests. The main process contributing to the total lateral flux out of Europe is the flux of reduced carbon compounds, corresponding to the sum of non-CO 2 gaseous species (CH 4 , CO, hydrocarbons,... ) emitted by ecosystems and exported out of the European boundary layer by the large scale atmospheric circulation. (authors)

Flux tubes in U(1) - Do they attract or repel each other?

International Nuclear Information System (INIS)

Zach, M.; Faber, M.; Skala, P.

1998-01-01

The dually transformed path integral of four-dimensional U(1) lattice gauge theory is used for a numerical investigation of multiply charged systems and the interaction between flux tubes. For this aim, it is convenient to implement periodically closed flux tubes (torelons) in the dual formulation. We calculate the free energy as well as the total electro-magnetic energy of doubly charged flux tubes as a function of the coupling β. The main results are that the string tension scales proportionally to the charge (contrary to the Coulomb potential) and in the range 0.9<β<1.0 we find a clear signal for attraction between flux tubes. (orig.)

The Role of Energy Flux in Weight Management

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Clemens Drenowatz

2017-12-01

Full Text Available Excess body weight has been identified as one of the leading threats to public health. In addition to health concerns at the individual level, the increased medical costs put a significant burden on the health care system. Even though an imbalance between energy intake and energy expenditure is ultimately responsible for changes in body weight and body composition such a simple opposition does not reflect the complex interaction of various contributors to energy balance. The limited understanding of the regulation of energy balance is also reflected by rising obesity levels, despite considerable efforts. In addition to energy balance, energy flux, which represents the rate of energy intake and energy expenditure, has been suggested to play an important role in the regulation of energy balance. Particularly a higher energy flux has been associated with a better matching of energy intake and energy expenditure resulting in stable body weight. While a high energy flux has been traditionally attributed to increased levels of physical activity it should be considered that increased body weight also affects energy expenditure and accordingly energy flux. In fact, total daily energy expenditure, and accordingly energy flux, does not differ between people in industrialized countries, living a predominantly sedentary lifestyle and people following a traditional lifestyle, characterized by high physical activity even though there are significant differences in body weight and body composition. It can, therefore, be argued that energy flux, rather than energy balance is a physiologically regulated entity. In this situation body weight and physical activity levels work in opposition to maintain a constant energy flux and weight gain would be a means to maintain a high energy flux with a sedentary lifestyle.

Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity.

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Hopperton, Kathryn E; Duncan, Robin E; Bazinet, Richard P; Archer, Michael C

2014-01-15

Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from (14)C-labeled acetate to those supplied exogenously as (14)C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2-3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

Fusion neutron yield and flux calculation on HT-7 superconducting tokamak

International Nuclear Information System (INIS)

Fu Yanzhang; Zhu Yubao; Chen Juequan

2006-01-01

Neutron yield and flux have been numerically estimated on HT-7 tokamak. The total fusion neutron yield and neutron flux distribution on different positions and azimuth angles of the device are presented. Analyses on the errors induced by ion temperature and density distribution factors are given in detail. The results of the calculations provide a useful database for neutron diagnostics and neutron radiation protection. (authors)

Effect of source integration on the geochemical fluxes from springs

International Nuclear Information System (INIS)

Frisbee, Marty D.; Phillips, Fred M.; White, Art F.; Campbell, Andrew R.; Liu, Fengjing

2013-01-01

Geochemical fluxes from watersheds are typically defined using mass-balance methods that essentially lump all weathering processes operative in a watershed into a single flux of solute mass measured in streamflow at the watershed outlet. However, it is important that we understand how weathering processes in different hydrological zones of a watershed (i.e., surface, unsaturated, and saturated zones) contribute to the total geochemical flux from the watershed. This capability will improve understanding of how geochemical fluxes from these different zones may change in response to climate change. Here, the geochemical flux from weathering processes occurring solely in the saturated zone is investigated. This task, however, remains exceedingly difficult due to the sparsity of subsurface sampling points, especially in large, remote, and/or undeveloped watersheds. In such cases, springflow is often assumed to be a proxy for groundwater (defined as water residing in fully saturated geologic formations). However, springflow generation may integrate different sources of water including, but not limited to, groundwater. The authors’ hypothesis is that long-term estimates of geochemical fluxes from groundwater using springflow proxies will be too large due to the integrative nature of springflow generation. Two conceptual models of springflow generation are tested using endmember mixing analyses (EMMA) on observations of spring chemistries and stable isotopic compositions in a large alpine watershed in the San Juan Mountains of southwestern Colorado. In the “total springflow” conceptual model, springflow is assumed to be 100% groundwater. In the “fractional springflow” conceptual model, springflow is assumed to be an integration of different sources of water (e.g., groundwater, unsaturated flow, preferential flow in the soil, etc.) and groundwater is only a fractional component. The results indicate that groundwater contributions in springflow range from 2% to 100

Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites.

Science.gov (United States)

Santangelo, G M; Tornow, J

1990-01-01

Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity. Images PMID:2405258

Long-term measurement of terpenoid flux above a Larix kaempferi forest using a relaxed eddy accumulation method

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Mochizuki, Tomoki; Tani, Akira; Takahashi, Yoshiyuki; Saigusa, Nobuko; Ueyama, Masahito

2014-02-01

Terpenoids emitted from forests contribute to the formation of secondary organic aerosols and affect the carbon budgets of forest ecosystems. To investigate seasonal variation in terpenoid flux involved in the aerosol formation and carbon budget, we measured the terpenoid flux of a Larix kaempferi forest between May 2011 and May 2012 by using a relaxed eddy accumulation method. Isoprene was emitted from a fern plant species Dryopteris crassirhizoma on the forest floor and monoterpenes from the L. kaempferi. α-Pinene was the dominant compound, but seasonal variation of the monoterpene composition was observed. High isoprene and monoterpene fluxes were observed in July and August. The total monoterpene flux was dependent on temperature, but several unusual high positive fluxes were observed after rain fall events. We found a good correlation between total monoterpene flux and volumetric soil water content (r = 0.88), and used this correlation to estimate monoterpene flux after rain events and calculate annual terpenoid emissions. Annual carbon emission in the form of total monoterpenes plus isoprene was determined to be 0.93% of the net ecosystem exchange. If we do not consider the effect of rain fall, carbon emissions may be underestimated by about 50%. Our results suggest that moisture conditions in the forest soil is a key factor controlling the monoterpene emissions from the forest ecosystem.

Characterization of total ecosystem-scale biogenic VOC exchange at a Mediterranean oak–hornbeam forest

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S. Schallhart

2016-06-01

Full Text Available Recently, the number and amount of biogenically emitted volatile organic compounds (VOCs has been discussed in great detail. Depending on the ecosystem, the published number varies between a dozen and several hundred compounds. We present ecosystem exchange fluxes from a mixed oak–hornbeam forest in the Po Valley, Italy. The fluxes were measured by a proton transfer reaction-time-of-flight (PTR-ToF mass spectrometer and calculated using the eddy covariance (EC method. Detectable fluxes were observed for up to 29 compounds, dominated by isoprene, which comprised over 60 % of the total upward flux (on a molar basis. The daily average of the total VOC upward flux was 10.4 nmol m−2 s−1. Methanol had the highest concentration and accounted for the largest downward flux. Methanol seemed to be deposited to dew, as the downward flux happened in the early morning, right after the calculated surface temperature came closest to the calculated dew point temperature.We estimated that up to 30 % of the upward flux of methyl vinyl ketone (MVK and methacrolein (MACR originated from atmospheric oxidation of isoprene. A comparison between two methods for the flux detection (manual and automated was made. Their respective advantages and disadvantages were discussed and the differences in their results shown. Both provide comparable results.

Histological and Physiological Alterations Induced by Thermal Neutron Fluxes in Male Swiss Albino Mice

International Nuclear Information System (INIS)

Alzergy, A.A.; Emara, N.M.; Abd El-Latif, A.A.; El-Saady, S.M.M.; Emara, N.M.; Abd El-Latif, A.A.

2010-01-01

This work was performed to investigate the biological effects of different thermal neutron fluxes (0.27x10 8 , 0.52X10 8 , 1.089X10 8 , 2.16X10 8 and 4.32X10 8 ) on liver and kidney of male mice using neutron irradiation cell with Ra-Be(α,n) 3 mCi neutron source Leybold (55930). Exposed to various fluxes of thermal neutron induced a dramatic alterations in hepatic and renal functions as indicated by biochemical estimation of several parameters (bilirubin, SGT, and alkaline phosphate .Urea , total protein, and albumin) and confirmed by histological examinations Thermal neutron exposure induces marked increase in the serum activities of total bilirubin, alanine amino transaminase (ALT or GPT), and alkaline phosphate, whereas, urea, total protein and albumin showed marked decline as compared to control group. The physiological changes induced in thermal neutron fluxes dependent manner. Histopathological results revealed mild to severe type of necrosis, and degenerative changes in liver and kidney of male mice exposed to thermal neutron fluxes. Also it was found that the histopathological alterations induced in thermal neutron fluxes dependent manner. It was found that exposed to thermal neutron fluxes irradiation plays prominent role in the development of the physiological alterations in male Swiss albino mice. The Former up normalities as a result of the sequence events followed interaction of radiation with the former biological mater (liver and kidney) of male Swiss albino mice, which are, physical, physicochemical, chemical, and biological stages.

Development of permeate flux model for municipal wastewater treatment using membrane bioreactor

International Nuclear Information System (INIS)

Geissler, S.; Zhou, H.; Zytner, R.; Melin, T.

2002-01-01

In municipal wastewater treatment, membrane filtration technologies receive great attention because they usually produce the better quality effluent, generate less sludge and require a smaller aeration tank volume. However, one main challenge of using membranes is membrane fouling, which results in a permeate flux decrease or transmembrane pressure increase over the time. Many efforts have been directed to develop the mechanistic permeate flux model to correlate the permeate flux with process parameters. However, their applicability has been largely thwarted due to complicated membrane fouling mechanisms and the interactions of many factors affecting the membrane bioreactor. This paper proposes a semi-empirical permeate flux model for the membrane bioreactor (MBR) process using ZENON immersed hollow fibre membrane modules. The semi-empirical model was proposed by assuming that the permeate flux is equal to transmembrane pressure divided by total resistance. The total resistance is divided into two components: an inside membrane resistance and an outer fouling layer resistance. These membrane resistances are then related to the ageing of membrane used. Good correlation was found between the predicted and measured flux, with the mean absolute deviation being less than 4%. The observations also identified some general rules for operating membrane systems. Ideally, it is advisable that high pressure periods be avoided as this leads to a faster increase of non-reversal membrane resistance. It was also observed that membrane preservatives should be washed out carefully prior to use. (author)

210 Pb fluxes in sediment layers sampled from Northern Patagonia lakes

International Nuclear Information System (INIS)

Ribeiro Guevara, S.; Sanchez, R.; Arribere, M.; Rizzo, A.

2003-01-01

Unsupported 210 Pb fluxes were determined from sediment core inventories in lakes located in Northern Patagonia, Argentina. Total 210 Pb, 226 Ra, associated with supported 210 Pb, and 137 Cs specific activity profiles were measured by gamma-ray spectrometry. Unsupported 210 Pb fluxes showed very low values when compared to other regions, with a 12 fold variation, ranging from 4 to 48 Bq m -2 x y -1 . The linear correlation observed between the 210 Pb fluxes and 137 Cs cumulative fluxes in sediment cores sampled from water bodies within a zone with similar precipitation demonstrated that both radioisotopes behave in the same manner in these systems concerning the processes occurred from fallout to sediment deposition, and that there are no appreciable local or regional sources of unsupported 210 Pb. Positive correlation of 210 Pb fluxes with organic matter contents of the uppermost sediment core layers was also observed. (author)

Quantifying Beetle-Mediated Effects on Gas Fluxes from Dung Pats

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Penttilä, Atte; Slade, Eleanor M.; Simojoki, Asko; Riutta, Terhi; Minkkinen, Kari; Roslin, Tomas

2013-01-01

Agriculture is one of the largest contributors of the anthropogenic greenhouse gases (GHGs) responsible for global warming. Measurements of gas fluxes from dung pats suggest that dung is a source of GHGs, but whether these emissions are modified by arthropods has not been studied. A closed chamber system was used to measure the fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from dung pats with and without dung beetles on a grass sward. The presence of dung beetles significantly affected the fluxes of GHGs from dung pats. Most importantly, fresh dung pats emitted higher amounts of CO2 and lower amounts of CH4 per day in the presence than absence of beetles. Emissions of N2O showed a distinct peak three weeks after the start of the experiment – a pattern detected only in the presence of beetles. When summed over the main grazing season (June–July), total emissions of CH4 proved significantly lower, and total emissions of N2O significantly higher in the presence than absence of beetles. While clearly conditional on the experimental conditions, the patterns observed here reveal a potential impact of dung beetles on gas fluxes realized at a small spatial scale, and thereby suggest that arthropods may have an overall effect on gas fluxes from agriculture. Dissecting the exact mechanisms behind these effects, mapping out the range of conditions under which they occur, and quantifying effect sizes under variable environmental conditions emerge as key priorities for further research. PMID:23940758

Optical performance evaluation of a solar furnace by measuring the highly concentrated solar flux

International Nuclear Information System (INIS)

Lee, Hyunjin; Chai, Kwankyo; Kim, Jongkyu; Lee, Sangnam; Yoon, Hwanki; Yu, Changkyun; Kang, Yongheack

2014-01-01

We evaluated optical performance of a solar furnace in the KIER (Korea Institute of Energy Research) by measuring the highly concentrated solar flux with the flux mapping method. We presented and analyzed optical performance in terms of concentrated solar flux distribution and power distribution. We investigated concentration ratio, stagnation temperature, total power, and concentration accuracy with help of a modeling code based on the ray tracing method and thereby compared with other solar furnaces. We also discussed flux changes by shutter opening angles and by position adjustment of reflector facets. In the course of flux analysis, we provided a better understanding of reference flux measurement for calibration, reflectivity measurement with a portable reflectometer, shadowing area consideration for effective irradiation, as well as accuracy and repeatability of flux measurements. The results in the present study will help proper utilization of a solar furnace by facilitating comparison between flux measurements at different conditions and flux estimation during operation

Obtaining of total and thermal neutron flux in the carousel facility of the TRIGA MARK IPR-R1 reactor using the Monte Carlo transport method

International Nuclear Information System (INIS)

Guerra, Bruno Teixeira

2011-01-01

The IPR-R1 is a reactor type TRIGA, Mark-I model, manufactured by the General Atomic Company and installed at Nuclear Technology Development Centre (CDTN) of Brazilian Nuclear Energy Commission (CNEN), in Belo Horizonte, Brazil. It is a light water moderated and cooled, graphite-reflected, open-pool type research reactor. IPR-R1 works at 100 kW but it will be briefly licensed to operate at 250 kW. It presents low power, low pressure, for application in research, training and radioisotopes production. The fuel is an alloy of zirconium hydride and uranium enriched at 20% in 235 U. The goal this work is modelling of the IPR-R1 Research Reactor TRIGA using the codes MCNPX2.6.0 (Monte Carlo N-Particle Transport extend) and MCNP5 to the calculating the neutron flux in the carousel facility. In each simulation the sample was placed in a different position, totaling forty positions around of the reactor core. The comparison between the results obtained with experimental values from other work showing a relatively good agreement. Moreover, this methodology is a theoretical tool in validating of the experimental values and necessary for determining neutron flux which can not be accessible experimentally. (author)

Solar Open Flux Migration from Pole to Pole: Magnetic Field Reversal.

Science.gov (United States)

Huang, G-H; Lin, C-H; Lee, L C

2017-08-25

Coronal holes are solar regions with low soft X-ray or low extreme ultraviolet intensities. The magnetic fields from coronal holes extend far away from the Sun, and thus they are identified as regions with open magnetic field lines. Coronal holes are concentrated in the polar regions during the sunspot minimum phase, and spread to lower latitude during the rising phase of solar activity. In this work, we identify coronal holes with outward and inward open magnetic fluxes being in the opposite poles during solar quiet period. We find that during the sunspot rising phase, the outward and inward open fluxes perform pole-to-pole trans-equatorial migrations in opposite directions. The migration of the open fluxes consists of three parts: open flux areas migrating across the equator, new open flux areas generated in the low latitude and migrating poleward, and new open flux areas locally generated in the polar region. All three components contribute to the reversal of magnetic polarity. The percentage of contribution from each component is different for different solar cycle. Our results also show that the sunspot number is positively correlated with the lower-latitude open magnetic flux area, but negatively correlated with the total open flux area.

Regional nitrogen budgets and riverine N & P fluxes for the drainages to the North Atlantic Ocean: Natural and human influences

Science.gov (United States)

Howarth, R.W.; Billen, G.; Swaney, D.; Townsend, A.; Jaworski, N.; Lajtha, K.; Downing, J.A.; Elmgren, Ragnar; Caraco, N.; Jordan, T.; Berendse, F.; Freney, J.; Kudeyarov, V.; Murdoch, P.; Zhu, Z.-L.

1996-01-01

We present estimates of total nitrogen and total phosphorus fluxes in rivers to the North Atlantic Ocean from 14 regions in North America, South America, Europe, and Africa which collectively comprise the drainage basins to the North Atlantic. The Amazon basin dominates the overall phosphorus flux and has the highest phosphorus flux per area. The total nitrogen flux from the Amazon is also large, contributing 3.3 Tg yr-1 out of a total for the entire North Atlantic region of 13.1 Tg yr-1. On a per area basis, however, the largest nitrogen fluxes are found in the highly disturbed watersheds around the North Sea, in northwestern Europe, and in the northeastern U.S., all of which have riverine nitrogen fluxes greater than 1,000 kg N km-2 yr-1. Non-point sources of nitrogen dominate riverine fluxes to the coast in all regions. River fluxes of total nitrogen from the temperate regions of the North Atlantic basin are correlated with population density, as has been observed previously for fluxes of nitrate in the world's major rivers. However, more striking is a strong linear correlation between river fluxes of total nitrogen and the sum of anthropogenically-derived nitrogen inputs to the temperate regions (fertilizer application, human-induced increases in atmospheric deposition of oxidized forms of nitrogen, fixation by leguminous crops, and the import/export of nitrogen in agricultural products). On average, regional nitrogen fluxes in rivers are only 25% of these anthropogenically derived nitrogen inputs. Denitrification in wetlands and aquatic ecosystems is probably the dominant sink, with storage in forests perhaps also of importance. Storage of nitrogen in groundwater, although of importance in some localities, is a very small sink for nitrogen inputs in all regions. Agricultural sources of nitrogen dominate inputs in many regions, particularly the Mississippi basin and the North Sea drainages. Deposition of oxidized nitrogen, primarily of industrial origin, is the

Coexisting Flux Rope and Dipped Arcade Sections Along One Solar Filament

Science.gov (United States)

Guo, Y.; Schmieder, B.; Démoulin, P.; Wiegelmann, T.; Aulanier, G.; Török, T.; Bommier, V.

2010-05-01

We compute the three-dimensional magnetic field of an active region in order to study the magnetic configuration of active region filaments. The nonlinear force-free field model is adopted to compute the magnetic field above the photosphere, where the vector magnetic field was observed by THEMIS/MTR on 2005 May 27. We propose a new method to remove the 180° ambiguity of the transverse field. Next, we analyze the implications of the preprocessing of the data by minimizing the total force and torque in the observed vector fields. This step provides a consistent bottom boundary condition for the nonlinear force-free field model. Then, using the optimization method to compute the coronal field, we find a magnetic flux rope along the polarity inversion line. The magnetic flux rope aligns well with part of an Hα filament, while the total distribution of the magnetic dips coincides with the whole Hα filament. This implies that the magnetic field structure in one section of the filament is a flux rope, while the other is a sheared arcade. The arcade induced a left-bearing filament in the magnetic field of negative helicity, which is opposite to the chirality of barbs that a flux rope would induce in a magnetic field of the same helicity sign. The field strength in the center of the flux rope is about 700 G, and the twist of the field lines is ~1.4 turns.

Design of a flux buffer based on the flux shuttle

International Nuclear Information System (INIS)

Gershenson, M.

1991-01-01

This paper discusses the design considerations for a flux buffer based on the flux-shuttle concept. Particular attention is given to the issues of flux popping, stability of operation and saturation levels for a large input. Modulation techniques used in order to minimize 1/f noise, in addition to offsets are also analyzed. Advantages over conventional approaches using a SQUID for a flux buffer are discussed. Results of computer simulations are presented

Restricting glycolysis impairs brown adipocyte glucose and oxygen consumption

DEFF Research Database (Denmark)

Winther, Sally; Isidor, Marie Sophie; Basse, Astrid Linde

2018-01-01

During thermogenic activation, brown adipocytes take up large amounts of glucose. In addition, cold stimulation leads to an upregulation of glycolytic enzymes. Here we have investigated the importance of glycolysis for brown adipocyte glucose consumption and thermogenesis. Using siRNA-mediated kn......During thermogenic activation, brown adipocytes take up large amounts of glucose. In addition, cold stimulation leads to an upregulation of glycolytic enzymes. Here we have investigated the importance of glycolysis for brown adipocyte glucose consumption and thermogenesis. Using si...... of glycolysis, i.e., hexokinase 2 (HK2) and pyruvate kinase M (PKM), respectively, decreased glucose uptake and ISO-stimulated oxygen consumption. HK2 knockdown had a more severe effect, which, in contrast to PKM knockdown, could not be rescued by supplementation with pyruvate. Hence, brown adipocytes rely...... on glucose consumption and glycolytic flux to achieve maximum thermogenic output, with glycolysis likely supporting thermogenesis not only by pyruvate formation but also by supplying intermediates for efferent metabolic pathways....

Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.

Directory of Open Access Journals (Sweden)

Nadine Gillmaier

Full Text Available The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13C-labelled glucose or glutamine as carbon tracers. The (13C-profiles of protein-derived amino acids from labelled host cells and intracellular L. monocytogenes identified active metabolic pathways in the different cell types. In the primary cells, infection with live L. monocytogenes increased glycolytic activity and enhanced flux of pyruvate into the TCA cycle via pyruvate dehydrogenase and pyruvate carboxylase, while in J774A.1 cells the already high glycolytic and glutaminolytic activities hardly changed upon infection. The carbon metabolism of intracellular L. monocytogenes was similar in both host cells. Taken together, the data suggest that efficient listerial replication in the cytosol of the host cells mainly depends on the glycolytic activity of the hosts.

Increased terrestrial to ocean sediment and carbon fluxes in the northern Chesapeake Bay associated with twentieth century land alteration

Science.gov (United States)

Saenger, C.; Cronin, T. M.; Willard, D.; Halka, J.; Kerhin, R.

2008-01-01

We calculated Chesapeake Bay (CB) sediment and carbon fluxes before and after major anthropogenic land clearance using robust monitoring, modeling and sedimentary data. Four distinct fluxes in the estuarine system were considered including (1) the flux of eroded material from the watershed to streams, (2) the flux of suspended sediment at river fall lines, (3) the burial flux in tributary sediments, and (4) the burial flux in main CB sediments. The sedimentary maximum in Ambrosia (ragweed) pollen marked peak land clearance (~1900 a.d.). Rivers feeding CB had a total organic carbon (TOC)/total suspended solids of 0.24??0.12, and we used this observation to calculate TOC fluxes from sediment fluxes. Sediment and carbon fluxes increased by 138-269% across all four regions after land clearance. Our results demonstrate that sediment delivery to CB is subject to significant lags and that excess post-land clearance sediment loads have not reached the ocean. Post-land clearance increases in erosional flux from watersheds, and burial in estuaries are important processes that must be considered to calculate accurate global sediment and carbon budgets. ?? 2008 Coastal and Estuarine Research Federation.

Accumulation of phosphorus in coastal marine sediments: relationship to benthic and diffusive fluxes

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Rocio Ponce

2010-11-01

Full Text Available Sedimentary phosphorus was characterized in sediment cores from 3 coastal ecosystems of the Gulf of Cadiz. High spatial variability was observed in total phosphorus (from 445 to 20291 μg g.sed-1 and in the other phosphorus phases studied. This variability correlates with the proximity of the 10 sampling stations to sources of urban and/or industrial effluent in the zone. The benthic and diffusive fluxes were measured concurrently with sediment collection at these stations. The measured values of benthic fluxes range between –14 and 6 mmol m-2 d-1. Generally, stations that showed increased interstitial phosphate concentrations with increasing depth were characterized by positive values in phosphate benthic fluxes and by high percentages of reactive forms of sedimentary phosphorus. Negative benthic fluxes were associated with stations receiving more anthropogenic matter, which showed progressively decreasing phosphate concentrations in the interstitial water with depth. In these anthropogenic areas, the non-reactive forms of phosphorus (those associated with ferric oxyhydroxide and authigenic carbonate fluorapatite are abundant, and reach values exceeding 75% of total phosphorus in sediment.

Magnetic-flux pump

Science.gov (United States)

Hildebrandt, A. F.; Elleman, D. D.; Whitmore, F. C. (Inventor)

1966-01-01

A magnetic flux pump is described for increasing the intensity of a magnetic field by transferring flux from one location to the magnetic field. The device includes a pair of communicating cavities formed in a block of superconducting material, and a piston for displacing the trapped magnetic flux into the secondary cavity producing a field having an intense flux density.

Soil greenhouse gas fluxes from different tree species on Taihang Mountain, North China

Science.gov (United States)

Liu, X. P.; Zhang, W. J.; Hu, C. S.; Tang, X. G.

2014-03-01

The objectives of this study were to investigate seasonal variation of greenhouse gas fluxes from soils on sites dominated by plantation (Robinia pseudoacacia, Punica granatum, and Ziziphus jujube) and natural regenerated forests (Vitex negundo var. heterophylla, Leptodermis oblonga, and Bothriochloa ischcemum), and to identify how tree species, litter exclusion, and soil properties (soil temperature, soil moisture, soil organic carbon, total N, soil bulk density, and soil pH) explained the temporal and spatial variation in soil greenhouse gas fluxes. Fluxes of greenhouse gases were measured using static chamber and gas chromatography techniques. Six static chambers were randomly installed in each tree species. Three chambers were randomly designated to measure the impacts of surface litter exclusion, and the remaining three were used as a control. Field measurements were conducted biweekly from May 2010 to April 2012. Soil CO2 emissions from all tree species were significantly affected by soil temperature, soil moisture, and their interaction. Driven by the seasonality of temperature and precipitation, soil CO2 emissions demonstrated a clear seasonal pattern, with fluxes significantly higher during the rainy season than during the dry season. Soil CH4 and N2O fluxes were not significantly correlated with soil temperature, soil moisture, or their interaction, and no significant seasonal differences were detected. Soil organic carbon and total N were significantly positively correlated with CO2 and N2O fluxes. Soil bulk density was significantly negatively correlated with CO2 and N2O fluxes. Soil pH was not correlated with CO2 and N2O emissions. Soil CH4 fluxes did not display pronounced dependency on soil organic carbon, total N, soil bulk density, and soil pH. Removal of surface litter significantly decreased in CO2 emissions and CH4 uptakes. Soils in six tree species acted as sinks for atmospheric CH4. With the exception of Ziziphus jujube, soils in all tree

Salp contributions to vertical carbon flux in the Sargasso Sea

Science.gov (United States)

Stone, Joshua P.; Steinberg, Deborah K.

2016-07-01

We developed a one-dimensional model to estimate salp contributions to vertical carbon flux at the Bermuda Atlantic Time-series Study (BATS) site in the North Atlantic subtropical gyre for a 17-yr period (April 1994 to December 2011). We based the model parameters on published rates of salp physiology and experimentally determined sinking and decomposition rates of salp carcasses. Salp grazing was low during non-bloom conditions, but routinely exceeded 100% of chlorophyll standing stock and primary production during blooms. Fecal pellet production was the largest source of salp carbon flux (78% of total), followed by respiration below 200 m (19%), sinking of carcasses (3%), and DOC excretion below 200 m (salp-mediated carbon flux. Seasonally, salp flux was higher during spring-summer than fall-winter, due to seasonal changes in species composition and abundance. Salp carbon export to 200 m was on average 2.3 mg C m-2 d-1 across the entire time series. This is equivalent to 11% of the mean 200 m POC flux measured by sediment traps in the region. During years with significant salp blooms, however, annually-averaged salp carbon export was the equivalent of up to 60% of trap POC flux at 200 m. Salp carbon flux attenuated slowly, and at 3200 m the average modeled carbon from salps was 109% of the POC flux measured in sediment traps at that depth. Migratory and carcass carbon export pathways should also be considered (alongside fecal pellet flux) as facilitating carbon export to sequestration depths in future studies.

Ozone flux over a Norway spruce forest and correlation with net ecosystem production

International Nuclear Information System (INIS)

Zapletal, Milos; Cudlin, Pavel; Chroust, Petr; Urban, Otmar; Pokorny, Radek; Edwards-Jonasova, Magda; Czerny, Radek; Janous, Dalibor; Taufarova, Klara; Vecera, Zbynek; Mikuska, Pavel; Paoletti, Elena

2011-01-01

Daily ozone deposition flux to a Norway spruce forest in Czech Republic was measured using the gradient method in July and August 2008. Results were in good agreement with a deposition flux model. The mean daily stomatal uptake of ozone was around 47% of total deposition. Average deposition velocity was 0.39 cm s -1 and 0.36 cm s -1 by the gradient method and the deposition model, respectively. Measured and modelled non-stomatal uptake was around 0.2 cm s -1 . In addition, net ecosystem production (NEP) was measured by using Eddy Covariance and correlations with O 3 concentrations at 15 m a.g.l., total deposition and stomatal uptake were tested. Total deposition and stomatal uptake of ozone significantly decreased NEP, especially by high intensities of solar radiation. - Highlights: → We estimate ozone deposition flux to a Norway spruce forest using the gradient method and model. → The mean stomatal uptake of ozone is approximately 47% of the total deposition. → We measure net ecosystem production (NEP) using Eddy Covariance. → We test whether elevated total deposition and stomatal uptake of O 3 imply a reduction of NEP. → Deposition and stomatal uptake of O 3 decrease NEP, especially by high intensities of solar radiation. - Net ecosystem production of a Norway spruce forest decreases with increasing deposition and stomatal uptake of ozone.

Effects of particle size and dry matter content of a total mixed ration on intraruminal equilibration and net portal flux of volatile fatty acids in lactating dairy cows

DEFF Research Database (Denmark)

Storm, Adam Christian; Kristensen, Niels Bastian

2010-01-01

Effects of physical changes in consistency of ruminal contents on intraruminal equilibration and net portal fluxes of volatile fatty acids (VFA) in dairy cows were studied. Four Danish Holstein cows (121 ± 17 d in milk, 591 ± 24 kg of body weight, mean ± SD) surgically fitted with a ruminal cannula...... and permanent indwelling catheters in the major splanchnic blood vessels were used. The experimental design was a 4 × 4 Latin square with a 2 × 2 factorial design of treatments. Treatments differed in forage (grass hay) particle size (FPS; 3.0 and 30 mm) and feed dry matter (DM) content of the total mixed...... ration (44.3 and 53.8%). The feed DM did not affect chewing time, ruminal variables, or net portal flux of VFA. However, decreasing the FPS decreased the overall chewing and rumination times by 151 ± 55 and 135 ± 29 min/d, respectively. No effect of the reduced chewing time was observed on ruminal p...

Limits to sustainable muscle performance: interaction between glycolysis and oxidative phosphorylation.

Science.gov (United States)

Conley, K E; Kemper, W F; Crowther, G J

2001-09-01

This paper proposes a mechanism responsible for setting the sustainable level of muscle performance. Our contentions are that the sustainable work rate is determined (i) at the muscle level, (ii) by the ability to maintain ATP supply and (iii) by the products of glycolysis that may inhibit the signal for oxidative phosphorylation. We argue below that no single factor 'limits' sustainable performance, but rather that the flux through and the interaction between glycolysis and oxidative phosphorylation set the level of sustainable ATP supply. This argument is based on magnetic resonance spectroscopy measurements of the sources and sinks for energy in vivo in human muscle and rattlesnake tailshaker muscle during sustained contractions. These measurements show that glycolysis provides between 20% (human muscle) and 40% (tailshaker muscle) of the ATP supply during sustained contractions in these muscles. We cite evidence showing that this high glycolytic flux does not reflect an O(2) limitation or mitochondria operating at their capacity. Instead, this flux reflects a pathway independent of oxidative phosphorylation for ATP supply during aerobic exercise. The consequence of this high glycolytic flux is accumulation of H(+), which we argue inhibits the rise in the signal activating oxidative phosphorylation, thereby restricting oxidative ATP supply to below the oxidative capacity. Thus, both glycolysis and oxidative phosphorylation play important roles in setting the highest steady-state ATP synthesis flux and thereby determine the sustainable level of work by exercising muscle.

Terrestrial water fluxes dominated by transpiration.

Science.gov (United States)

Jasechko, Scott; Sharp, Zachary D; Gibson, John J; Birks, S Jean; Yi, Yi; Fawcett, Peter J

2013-04-18

Renewable fresh water over continents has input from precipitation and losses to the atmosphere through evaporation and transpiration. Global-scale estimates of transpiration from climate models are poorly constrained owing to large uncertainties in stomatal conductance and the lack of catchment-scale measurements required for model calibration, resulting in a range of predictions spanning 20 to 65 per cent of total terrestrial evapotranspiration (14,000 to 41,000 km(3) per year) (refs 1, 2, 3, 4, 5). Here we use the distinct isotope effects of transpiration and evaporation to show that transpiration is by far the largest water flux from Earth's continents, representing 80 to 90 per cent of terrestrial evapotranspiration. On the basis of our analysis of a global data set of large lakes and rivers, we conclude that transpiration recycles 62,000 ± 8,000 km(3) of water per year to the atmosphere, using half of all solar energy absorbed by land surfaces in the process. We also calculate CO2 uptake by terrestrial vegetation by connecting transpiration losses to carbon assimilation using water-use efficiency ratios of plants, and show the global gross primary productivity to be 129 ± 32 gigatonnes of carbon per year, which agrees, within the uncertainty, with previous estimates. The dominance of transpiration water fluxes in continental evapotranspiration suggests that, from the point of view of water resource forecasting, climate model development should prioritize improvements in simulations of biological fluxes rather than physical (evaporation) fluxes.

Ultraviolet Flux Variation of Epsilon Aurigae

Directory of Open Access Journals (Sweden)

Young Woon Kang

1990-06-01

Full Text Available The eighteen ultraviolet light curves of Epsilon Aurigae have been plotted using the integrated fluxes reduced from the 233 IUE low dispersion spectra taken between 1978 and 1986. The times of contacts and depth of eclipse have been determined from the light curves at the wavelength from 2550 Å to 3050 Å. The UV light curves show two brightenings during the totality, the downward slope of the variation from the second to the third contacts, and asymmetry of the eclipse light curve. The two selected spectra note that the energy density distribution is not changed between the totality of the eclipse and out-of-eclipse.

OpenFLUX: efficient modelling software for 13C-based metabolic flux analysis

Directory of Open Access Journals (Sweden)

Nielsen Lars K

2009-05-01

Full Text Available Abstract Background The quantitative analysis of metabolic fluxes, i.e., in vivo activities of intracellular enzymes and pathways, provides key information on biological systems in systems biology and metabolic engineering. It is based on a comprehensive approach combining (i tracer cultivation on 13C substrates, (ii 13C labelling analysis by mass spectrometry and (iii mathematical modelling for experimental design, data processing, flux calculation and statistics. Whereas the cultivation and the analytical part is fairly advanced, a lack of appropriate modelling software solutions for all modelling aspects in flux studies is limiting the application of metabolic flux analysis. Results We have developed OpenFLUX as a user friendly, yet flexible software application for small and large scale 13C metabolic flux analysis. The application is based on the new Elementary Metabolite Unit (EMU framework, significantly enhancing computation speed for flux calculation. From simple notation of metabolic reaction networks defined in a spreadsheet, the OpenFLUX parser automatically generates MATLAB-readable metabolite and isotopomer balances, thus strongly facilitating model creation. The model can be used to perform experimental design, parameter estimation and sensitivity analysis either using the built-in gradient-based search or Monte Carlo algorithms or in user-defined algorithms. Exemplified for a microbial flux study with 71 reactions, 8 free flux parameters and mass isotopomer distribution of 10 metabolites, OpenFLUX allowed to automatically compile the EMU-based model from an Excel file containing metabolic reactions and carbon transfer mechanisms, showing it's user-friendliness. It reliably reproduced the published data and optimum flux distributions for the network under study were found quickly ( Conclusion We have developed a fast, accurate application to perform steady-state 13C metabolic flux analysis. OpenFLUX will strongly facilitate and

Communication: On the calculation of time-dependent electron flux within the Born-Oppenheimer approximation: A flux-flux reflection principle

Science.gov (United States)

Albert, Julian; Hader, Kilian; Engel, Volker

2017-12-01

It is commonly assumed that the time-dependent electron flux calculated within the Born-Oppenheimer (BO) approximation vanishes. This is not necessarily true if the flux is directly determined from the continuity equation obeyed by the electron density. This finding is illustrated for a one-dimensional model of coupled electronic-nuclear dynamics. There, the BO flux is in perfect agreement with the one calculated from a solution of the time-dependent Schrödinger equation for the coupled motion. A reflection principle is derived where the nuclear BO flux is mapped onto the electronic flux.

Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape During In Vitro Culture

Energy Technology Data Exchange (ETDEWEB)

Schwender, Jorg [Brookhaven National Lab. (BNL), Upton, NY (United States); Hebbelmann, Inga [Brookhaven National Lab. (BNL), Upton, NY (United States); Heinzel, Nicholas [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany); Hildebrandt, Tatjana [Univ. of Hannover (Germany); Rogers, Alistair [Brookhaven National Lab. (BNL), Upton, NY (United States); Naik, Dhiraj [Brookhaven National Lab. (BNL), Upton, NY (United States); Indian Inst. of Advanced Research Koba, Gujarat (India); Klapperstuck, Matthias [Monash Univ., Melbourne, VIC (Australia); Braun, Hans -Peter [Univ. of Hannover (Germany); Schreiber, Falk [Monash Univ., Melbourne, VIC (Australia); Univ. Halle-Wittenberg, Melbourne (Australia); Denolf, Peter [Bayer CropScience (Belgium); Borisjuk, Ljudmilla [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany); Rolletschek, Hardy [Leibniz Inst. of Plant Genetics and Crop Plant Research, Gatersleben (Germany)

2015-07-01

Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. We observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Also, quantitative data were used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism..

Bayesian calibration of reactor neutron flux spectrum using activation detectors measurements: Application to CALIBAN reactor

International Nuclear Information System (INIS)

Cartier, J.; Casoli, P.; Chappert, F.

2013-01-01

In this paper, we present calibration methods in order to estimate reactor neutron flux spectrum and its uncertainties by using integral activation measurements. These techniques are performed using Bayesian and MCMC framework. These methods are applied to integral activation experiments in the cavity of the CALIBAN reactor. We estimate the neutron flux and its related uncertainties. The originality of this work is that these uncertainties take into account measurements uncertainties, cross-sections uncertainties and model error. In particular, our results give a very good approximation of the total flux and indicate that neutron flux from MCNP simulation for energies above about 5 MeV seems to overestimate the 'real flux'. (authors)

Influence of slow-release urea on nitrogen balance and portal-drained visceral nutrient flux in beef steers

DEFF Research Database (Denmark)

Taylor-Edwards, C C; Elam, N A; Kitts, S E

2009-01-01

Two experiments were conducted to evaluate the effects of slow-release urea (SRU) versus feed-grade urea on portal-drained visceral (PDV) nutrient flux, nutrient digestibility, and total N balance in beef steers.......Two experiments were conducted to evaluate the effects of slow-release urea (SRU) versus feed-grade urea on portal-drained visceral (PDV) nutrient flux, nutrient digestibility, and total N balance in beef steers....

Upper limits on the total cosmic-ray luminosity of individual sources

Energy Technology Data Exchange (ETDEWEB)

Anjos, R.C.; De Souza, V. [Instituto de Física de São Carlos, Universidade de São Paulo, São Paulo (Brazil); Supanitsky, A.D., E-mail: rita@ifsc.usp.br, E-mail: vitor@ifsc.usp.br, E-mail: supanitsky@iafe.uba.ar [Instituto de Astronomía y Física del Espacio (IAFE), CONICET-UBA, Buenos Aires (Argentina)

2014-07-01

In this paper, upper limits on the total luminosity of ultra-high-energy cosmic-rays (UHECR) E > 10{sup 18} eV) are determined for five individual sources. The upper limit on the integral flux of GeV--TeV gamma-rays is used to extract the upper limit on the total UHECR luminosity of individual sources. The correlation between upper limit on the integral GeV--TeV gamma-ray flux and upper limit on the UHECR luminosity is established through the cascading process that takes place during propagation of the cosmic rays in the background radiation fields, as explained in reference [1]. Twenty-eight sources measured by FERMI-LAT, VERITAS and MAGIC observatories have been studied. The measured upper limit on the GeV--TeV gamma-ray flux is restrictive enough to allow the calculation of an upper limit on the total UHECR cosmic-ray luminosity of five sources. The upper limit on the UHECR cosmic-ray luminosity of these sources is shown for several assumptions on the emission mechanism. For all studied sources an upper limit on the ultra-high-energy proton luminosity is also set.

Upper limits on the total cosmic-ray luminosity of individual sources

International Nuclear Information System (INIS)

Anjos, R.C.; De Souza, V.; Supanitsky, A.D.

2014-01-01

In this paper, upper limits on the total luminosity of ultra-high-energy cosmic-rays (UHECR) E > 10 18 eV) are determined for five individual sources. The upper limit on the integral flux of GeV--TeV gamma-rays is used to extract the upper limit on the total UHECR luminosity of individual sources. The correlation between upper limit on the integral GeV--TeV gamma-ray flux and upper limit on the UHECR luminosity is established through the cascading process that takes place during propagation of the cosmic rays in the background radiation fields, as explained in reference [1]. Twenty-eight sources measured by FERMI-LAT, VERITAS and MAGIC observatories have been studied. The measured upper limit on the GeV--TeV gamma-ray flux is restrictive enough to allow the calculation of an upper limit on the total UHECR cosmic-ray luminosity of five sources. The upper limit on the UHECR cosmic-ray luminosity of these sources is shown for several assumptions on the emission mechanism. For all studied sources an upper limit on the ultra-high-energy proton luminosity is also set

Soil CO2 flux in response to wheel traffic in a no-till system

Science.gov (United States)

Measurements of soil CO2 flux in the absence of living plants can be used to evaluate the effectiveness of soil management practices for C sequestration, but field CO2 flux is spatially variable and may be affected by soil compaction and percentage of total pore space filled with water (%WFPS). The ...

STUDY OF THE POYNTING FLUX IN ACTIVE REGION 10930 USING DATA-DRIVEN MAGNETOHYDRODYNAMIC SIMULATION

International Nuclear Information System (INIS)

Fan, Y. L.; Wang, H. N.; He, H.; Zhu, X. S.

2011-01-01

Powerful solar flares are closely related to the evolution of magnetic field configuration on the photosphere. We choose the Poynting flux as a parameter in the study of magnetic field changes. We use time-dependent multidimensional MHD simulations around a flare occurrence to generate the results, with the temporal variation of the bottom boundary conditions being deduced from the projected normal characteristic method. By this method, the photospheric magnetogram could be incorporated self-consistently as the bottom condition of data-driven simulations. The model is first applied to a simulation datum produced by an emerging magnetic flux rope as a test case. Then, the model is used to study NOAA AR 10930, which has an X3.4 flare, the data of which has been obtained by the Hinode/Solar Optical Telescope on 2006 December 13. We compute the magnitude of Poynting flux (S total ), radial Poynting flux (S z ), a proxy for ideal radial Poynting flux (S proxy ), Poynting flux due to plasma surface motion (S sur ), and Poynting flux due to plasma emergence (S emg ) and analyze their extensive properties in four selected areas: the whole sunspot, the positive sunspot, the negative sunspot, and the strong-field polarity inversion line (SPIL) area. It is found that (1) the S total , S z , and S proxy parameters show similar behaviors in the whole sunspot area and in the negative sunspot area. The evolutions of these three parameters in the positive area and the SPIL area are more volatile because of the effect of sunspot rotation and flux emergence. (2) The evolution of S sur is largely influenced by the process of sunspot rotation, especially in the positive sunspot. The evolution of S emg is greatly affected by flux emergence, especially in the SPIL area.

Influence of grain boundary connectivity on the trapped magnetic flux of multi-seeded bulk superconductors

Science.gov (United States)

Deng, Z.; Miki, M.; Felder, B.; Tsuzuki, K.; Shinohara, N.; Hara, S.; Uetake, T.; Izumi, M.

2011-09-01

The top-seeded melt-growth process with multi-seeding technique provides a promising way to fabricate large-sized bulk superconductors in an economical way. To understand the essential characteristics of the multi-seeded bulks, the paper reports the influence of the grain boundary (GB) coupling or connectivity on the total trapped magnetic flux. The coupling ratio, the lowest trapped flux density in the GB area to the averaged top value of the two neighboring peak trapped fields, is introduced to reflect the coupling quality of GBs inside a multi-seeded bulk. By the trapped flux density measurement of four different performance multi-seeded YBCO bulk samples as representatives, it was found that the GB coupling plays an important role for the improvement of the total trapped magnetic flux; moreover, somewhat more significant than the widely used parameter of the peak trapped fields to evaluate the physical performance of bulk samples. This characteristic is different with the case of the well-grown single-grain bulks.

Variability of the Lyman alpha flux with solar activity

International Nuclear Information System (INIS)

Lean, J.L.; Skumanich, A.

1983-01-01

A three-component model of the solar chromosphere, developed from ground based observations of the Ca II K chromospheric emission, is used to calculate the variability of the Lyman alpha flux between 1969 and 1980. The Lyman alpha flux at solar minimum is required in the model and is taken as 2.32 x 10 11 photons/cm 2 /s. This value occurred during 1975 as well as in 1976 near the commencement of solar cycle 21. The model predicts that the Lyman alpha flux increases to as much as 5 x 10 11 photons/cm 2 /s at the maximum of the solar cycle. The ratio of the average fluxes for December 1979 (cycle maximum) and July 1976 (cycle minimum) is 1.9. During solar maximum the 27-day solar rotation is shown to cause the Lyman alpha flux to vary by as much as 40% or as little as 5%. The model also shows that the Lyman alpha flux varies over intermediate time periods of 2 to 3 years, as well as over the 11-year sunspot cycle. We conclude that, unlike the sunspot number and the 10.7-cm radio flux, the Lyman alpha flux had a variability that was approximately the same during each of the past three cycles. Lyman alpha fluxes calculated by the model are consistent with measurements of the Lyman alpha flux made by 11 of a total of 14 rocket experiments conducted during the period 1969--1980. The model explains satisfactorily the absolute magnitude, long-term trends, and the cycle variability seen in the Lyman alpha irradiances by the OSO 5 satellite experiment. The 27-day variability observed by the AE-E satellite experiment is well reproduced. However, the magntidue of the AE-E 1 Lyman alpha irradiances are higher than the model calculations by between 40% and 80%. We suggest that the assumed calibration of the AE-E irradiances is in error

Flux flow and flux creep in thick films of YBCO. [Y-Ba-Cu-O

Energy Technology Data Exchange (ETDEWEB)

Rickets, J.; Vinen, W.F.; Abell, J.S.; Shields, T.C. (Superconductivity Research Group, Univ. of Birmingham (United Kingdom))

1991-12-01

The results are described of new experiments designed to study flux creep and flux flow along a single flux percolation path in thick films of YBCO. The flux flow regime is studied by a four-point resistive technique using pulsed currents, and the flux creep regime by observing the rate at which flux enters a superconducting loop in parallel with the resistance that is associated with the flux percolation path. (orig.).

MAGNETIC FLUX TRANSPORT AND THE LONG-TERM EVOLUTION OF SOLAR ACTIVE REGIONS

International Nuclear Information System (INIS)

Ugarte-Urra, Ignacio; Upton, Lisa; Warren, Harry P.; Hathaway, David H.

2015-01-01

With multiple vantage points around the Sun, Solar Terrestrial Relations Observatory (STEREO) and Solar Dynamics Observatory imaging observations provide a unique opportunity to view the solar surface continuously. We use He ii 304 Å data from these observatories to isolate and track ten active regions and study their long-term evolution. We find that active regions typically follow a standard pattern of emergence over several days followed by a slower decay that is proportional in time to the peak intensity in the region. Since STEREO does not make direct observations of the magnetic field, we employ a flux-luminosity relationship to infer the total unsigned magnetic flux evolution. To investigate this magnetic flux decay over several rotations we use a surface flux transport model, the Advective Flux Transport model, that simulates convective flows using a time-varying velocity field and find that the model provides realistic predictions when information about the active region's magnetic field strength and distribution at peak flux is available. Finally, we illustrate how 304 Å images can be used as a proxy for magnetic flux measurements when magnetic field data is not accessible

Lithium prevents early cytosolic calcium increase and secondary injurious calcium overload in glycolytically inhibited endothelial cells

Energy Technology Data Exchange (ETDEWEB)

Bosche, Bert, E-mail: bert.bosche@uk-essen.de [Department of Neurology, University of Duisburg-Essen (Germany); Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Schäfer, Matthias, E-mail: matthias.schaefer@sanofi.com [Institute of Physiology, Justus-Liebig-University Giessen (Germany); Graf, Rudolf, E-mail: rudolf.graf@nf.mpg.de [Max Planck Institute for Neurological Research with Klaus-Joachim-Zülch Laboratories of the Max Planck Society and the Medical Faculty of the University of Cologne (Germany); Härtel, Frauke V., E-mail: frauke.haertel@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany); Schäfer, Ute, E-mail: ute.schaefer@medunigraz.at [Research Unit for Experimental Neurotraumatology, Medical University of Graz (Austria); Noll, Thomas, E-mail: thomas.noll@tu-dresden.de [Institute of Physiology, Medical Faculty Carl Gustav Carus, Technical University Dresden (Germany)

2013-05-03

Highlights: •We investigate free calcium as a central signalling element in endothelial cells. •Inhibition of glycolysis with 2-deoxy-D-glucose reduces cellular ATP. •This manoeuvre leads to a biphasic increase and overload of free calcium. •Pre-treatment with lithium for 24 h abolishes both phases of the calcium increase. •This provides a new strategy to protect endothelial calcium homeostasis and barrier function. -- Abstract: Cytosolic free calcium concentration ([Ca{sup 2+}]{sub i}) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca{sup 2+}]{sub i} overload, which results in barrier failure. In a model of cultured porcine aortic endothelial monolayers (EC), we addressed the question of whether [Ca{sup 2+}]{sub i} overload can be prevented by lithium treatment. [Ca{sup 2+}]{sub i} and ATP were analysed using Fura-2 and HPLC, respectively. The combined inhibition of glycolytic and mitochondrial ATP synthesis by 2-desoxy-D-glucose (5 mM; 2-DG) plus sodium cyanide (5 mM; NaCN) caused a significant decrease in cellular ATP content (14 ± 1 nmol/mg protein vs. 18 ± 1 nmol/mg protein in the control, n = 6 culture dishes, P < 0.05), an increase in [Ca{sup 2+}]{sub i} (278 ± 24 nM vs. 71 ± 2 nM in the control, n = 60 cells, P < 0.05), and the formation of gaps between adjacent EC. These observations indicate that there is impaired barrier function at an early state of metabolic inhibition. Glycolytic inhibition alone by 10 mM 2-DG led to a similar decrease in ATP content (14 ± 2 nmol/mg vs. 18 ± 1 nmol/mg in the control, P < 0.05) with a delay of 5 min. The [Ca{sup 2+}]{sub i} response of EC was biphasic with a peak after 1 min (183 ± 6 nM vs. 71 ± 1 nM, n = 60 cells, P < 0.05) followed by a sustained increase in [Ca{sup 2+}]{sub i}. A 24-h pre-treatment with 10 mM of lithium

Characterization of total ecosystem scale biogenic VOC exchange at a Mediterranean oak-hornbeam forest

Science.gov (United States)

Schallhart, S.; Rantala, P.; Nemitz, E.; Mogensen, D.; Tillmann, R.; Mentel, T. F.; Rinne, J.; Ruuskanen, T. M.

2015-10-01

Recently, the number and amount of biogenically emitted volatile organic compounds (VOCs) has been discussed vigorously. Depending on the ecosystem the published number varies between a dozen and several hundred compounds. We present ecosystem exchange fluxes from a mixed oak-hornbeam forest in the Po Valley, Italy. The fluxes were measured by a proton transfer reaction-time-of-flight (PTR-ToF) mass spectrometer and calculated by the eddy covariance (EC) method. Detectable fluxes were observed for twelve compounds, dominated by isoprene, which comprised over 65 % of the total flux emission. The daily average of the total VOC emission was 9.5 nmol m-2 s-1. Methanol had the highest concentration and accounted for the largest deposition. Methanol seemed to be deposited to dew, as the deposition happened in the early morning, right after the calculated surface temperature came closest to the calculated dew point temperature. We estimated that up to 27 % of the upward flux of methyl vinyl ketone (MVK) and methacrolein (MACR) originated from atmospheric oxidation of isoprene. A comparison between two flux detection methods (classical/visual and automated) was made. Their respective advantages and disadvantages were discussed and the differences in their results shown. Both provide comparable results; however we recommend the automated method with a compound filter, which combines the fast analysis and better flux detection, without the overestimation due to double counting.

Estimation of Throughfall and Stemflow Bacterial Flux in a Subtropical Oak-Cedar Forest

Science.gov (United States)

Bittar, Thais B.; Pound, Preston; Whitetree, Ansley; Moore, L. Dean; Van Stan, John T.

2018-02-01

Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to affect microbially mediated biogeochemical processes. Novel data on bacterial fluxes from the phyllosphere to the pedosphere during rainfall via throughfall (rain dripping from/through the canopy) and stemflow (rain funneled down tree stems) are reported. Bacterial concentrations were quantified using flow cytometry and validated with quantitative polymerase chain reaction assays in rainfall samples from an oak-cedar forest in coastal Georgia (southeastern U.S.). Bacteria concentrations (cells mL-1) and storm-normalized fluxes (cells m-2 h-1, cells m-2 mm-1) were greater for cedar versus oak. Total bacterial flux was 1.5 × 1016 cells ha-1 yr-1. These previously unexamined bacterial fluxes are interpreted in the context of major elemental pools and fluxes in forests and could represent inoculum-level sources of bacteria (if alive), and organic matter and inorganic solute inputs (if lysed) to soils.

Methane Fluxes in West Siberia: 3-D Regional Model Simulation

International Nuclear Information System (INIS)

Jagovkina, S. V.; Karol, I. L.; Zubov, V. A.; Lagun, V. E.; Reshetnikov, A. I.; Rozanov, E. V.

2001-01-01

The West Siberian region is one of the main contributors of the atmospheric greenhouse gas methane due to the large areas of wetlands, rivers, lakes and numerous gas deposits situated there.But there are no reliable estimations of integral methane flux from this area into the atmosphere. For assessment of methane fluxes in West Siberia the specially constructed 3-D regional chemical transport model was applied. The 3-D distribution of methane is calculated on the basis of the current meteorological data fields(wind, temperature, geopotential) updated 4 times a day. The methane concentrations measured near the main gas fields of West Siberia in the summer season of 1999, were used for correction of methane flux intensity estimates obtained previously by comparison of measurements carried out in summer 1993 and 1996 with modelled methane mixing ratio distribution. This set of field and model experiments confirmed the preliminary conclusion about low leakage intensity: anthropogenic methane flux does not exceed 5-15% of total summer methane flux, estimated as 11-12 Mt CH 4 in summer from this region, in spite of the large areas of gas deposits located there

DETECTION OF FLUX EMERGENCE, SPLITTING, MERGING, AND CANCELLATION OF NETWORK FIELD. I. SPLITTING AND MERGING

Energy Technology Data Exchange (ETDEWEB)

Iida, Y.; Yokoyama, T. [Department of Earth and Planetary Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan); Hagenaar, H. J. [Lockheed Martin Advanced Technology Center, Org. ADBS, Building 252, 3251 Hanover Street, Palo Alto, CA 94304 (United States)

2012-06-20

Frequencies of magnetic patch processes on the supergranule boundary, namely, flux emergence, splitting, merging, and cancellation, are investigated through automatic detection. We use a set of line-of-sight magnetograms taken by the Solar Optical Telescope (SOT) on board the Hinode satellite. We found 1636 positive patches and 1637 negative patches in the data set, whose time duration is 3.5 hr and field of view is 112'' Multiplication-Sign 112''. The total numbers of magnetic processes are as follows: 493 positive and 482 negative splittings, 536 positive and 535 negative mergings, 86 cancellations, and 3 emergences. The total numbers of emergence and cancellation are significantly smaller than those of splitting and merging. Further, the frequency dependence of the merging and splitting processes on the flux content are investigated. Merging has a weak dependence on the flux content with a power-law index of only 0.28. The timescale for splitting is found to be independent of the parent flux content before splitting, which corresponds to {approx}33 minutes. It is also found that patches split into any flux contents with the same probability. This splitting has a power-law distribution of the flux content with an index of -2 as a time-independent solution. These results support that the frequency distribution of the flux content in the analyzed flux range is rapidly maintained by merging and splitting, namely, surface processes. We suggest a model for frequency distributions of cancellation and emergence based on this idea.

Self-shielding coefficient and thermal flux depression factor of voluminous sample in neutron activation analysis

International Nuclear Information System (INIS)

Noorddin Ibrahim; Rosnie Akang

2009-01-01

Full text: One of the major problems encountered during the irradiation of large inhomogeneous samples in performing activation analysis using neutron is the perturbation of the neutron field due to absorption and scattering of neutron within the sample as well as along the neutron guide in the case of prompt gamma activation analysis. The magnitude of this perturbation shown by self-shielding coefficient and flux depression depend on several factors including the average neutron energy, the size and shape of the sample, as well as the macroscopic absorption cross section of the sample. In this study, we use Monte Carlo N-Particle codes to simulate the variation of neutron self-shielding coefficient and thermal flux depression factor as a function of the macroscopic thermal absorption cross section. The simulation works was carried out using the high performance computing facility available at UTM while the experimental work was performed at the tangential beam port of Reactor TRIGA PUSPATI, Malaysia Nuclear Agency. The neutron flux measured along the beam port is found to be in good agreement with the simulated data. Our simulation results also reveal that total flux perturbation factor decreases as the value of absorption increases. This factor is close to unity for low absorbing sample and tends towards zero for strong absorber. In addition, sample with long mean chord length produces smaller flux perturbation than the shorter mean chord length. When comparing both the graphs of self-shielding factor and total disturbance, we can conclude that the total disturbance of the thermal neutron flux on the large samples is dominated by the self-shielding effect. (Author)

Influence of grain boundary connectivity on the trapped magnetic flux of multi-seeded bulk superconductors

Energy Technology Data Exchange (ETDEWEB)

Deng, Z., E-mail: zgdeng@gmail.com [Laboratory of Applied Physics, Department of Marine Electronics and Mechanical Engineering, Tokyo University of Marine Science and Technology, Tokyo 135-8533 (Japan); Miki, M.; Felder, B.; Tsuzuki, K.; Shinohara, N.; Hara, S.; Uetake, T.; Izumi, M. [Laboratory of Applied Physics, Department of Marine Electronics and Mechanical Engineering, Tokyo University of Marine Science and Technology, Tokyo 135-8533 (Japan)

2011-09-15

Four different performance multi-seeded YBCO bulks as representatives. A coupling ratio to reflect the coupling quality of GBs inside multi-seeded bulks. An averaged trapped magnetic flux density parameter was introduced. The top-seeded melt-growth process with multi-seeding technique provides a promising way to fabricate large-sized bulk superconductors in an economical way. To understand the essential characteristics of the multi-seeded bulks, the paper reports the influence of the grain boundary (GB) coupling or connectivity on the total trapped magnetic flux. The coupling ratio, the lowest trapped flux density in the GB area to the averaged top value of the two neighboring peak trapped fields, is introduced to reflect the coupling quality of GBs inside a multi-seeded bulk. By the trapped flux density measurement of four different performance multi-seeded YBCO bulk samples as representatives, it was found that the GB coupling plays an important role for the improvement of the total trapped magnetic flux; moreover, somewhat more significant than the widely used parameter of the peak trapped fields to evaluate the physical performance of bulk samples. This characteristic is different with the case of the well-grown single-grain bulks.

Bayesian calibration of reactor neutron flux spectrum using activation detectors measurements: Application to CALIBAN reactor

Energy Technology Data Exchange (ETDEWEB)

Cartier, J. [Commissariat a l' Energie Atomique et aux Energies Alternatives CEA, DAM, DIF, F-91297 Arpajon (France); Casoli, P. [Commissariat a l' Energie Atomique et aux Energies Alternatives CEA, DAM, Valduc, F-21120 Is sur Tille (France); Chappert, F. [Commissariat a l' Energie Atomique et aux Energies Alternatives CEA, DAM, DIF, F-91297 Arpajon (France)

2013-07-01

In this paper, we present calibration methods in order to estimate reactor neutron flux spectrum and its uncertainties by using integral activation measurements. These techniques are performed using Bayesian and MCMC framework. These methods are applied to integral activation experiments in the cavity of the CALIBAN reactor. We estimate the neutron flux and its related uncertainties. The originality of this work is that these uncertainties take into account measurements uncertainties, cross-sections uncertainties and model error. In particular, our results give a very good approximation of the total flux and indicate that neutron flux from MCNP simulation for energies above about 5 MeV seems to overestimate the 'real flux'. (authors)

Annual and seasonal CO2 fluxes from Russian southern taiga soils

International Nuclear Information System (INIS)

Kurganova, I.; Lopes De Gerenyu, V.; Rozanova, L.; Sapronov, D.; Myakshina, T.; Kudeyarov, V.

2003-01-01

Annual and seasonal characteristics of CO 2 emission from five different ecosystems were studied in situ (Russia, Moscow Region) from November 1997 through October 2000. The annual behaviour of the soil respiration rate is influenced by weather conditions during a particular year. Annual CO 2 fluxes from the soils depend on land use of the soils and averaged 684 and 906 g C/m 2 from sandy Albeluvisols (sod-podzolic soils) under forest and grassland, respectively. Annual emission from clay Phaeozems (grey forest soils) was lower and ranged from 422 to 660 g C/m 2 ; the order of precedence was arable 2 fluxes caused by weather conditions ranged from 18% (forest ecosystem on Phaeozems) to 31% (agro-ecosystem). The contribution from the cold period (with snow, November-April) to the annual CO 2 flux was substantial and averaged 21% and 14% for natural and agricultural ecosystems, respectively. The CO 2 fluxes comprised approximately 48-51% in summer, 23-24% in autumn, 18-20% in spring and 7-10% in winter of the total annual carbon dioxide flux

Global constraints on Z2 fluxes in two different anisotropic limits of a hypernonagon Kitaev model

Science.gov (United States)

Kato, Yasuyuki; Kamiya, Yoshitomo; Nasu, Joji; Motome, Yukitoshi

2018-05-01

The Kitaev model is an exactly-soluble quantum spin model, whose ground state provides a canonical example of a quantum spin liquid. Spin excitations from the ground state are fractionalized into emergent matter fermions and Z2 fluxes. The Z2 flux excitation is pointlike in two dimensions, while it comprises a closed loop in three dimensions because of the local constraint for each closed volume. In addition, the fluxes obey global constraints involving (semi)macroscopic number of fluxes. We here investigate such global constraints in the Kitaev model on a three-dimensional lattice composed of nine-site elementary loops, dubbed the hypernonagon lattice, whose ground state is a chiral spin liquid. We consider two different anisotropic limits of the hypernonagon Kitaev model where the low-energy effective models are described solely by the Z2 fluxes. We show that there are two kinds of global constraints in the model defined on a three-dimensional torus, namely, surface and volume constraints: the surface constraint is imposed on the even-odd parity of the total number of fluxes threading a two-dimensional slice of the system, while the volume constraint is for the even-odd parity of the number of the fluxes through specific plaquettes whose total number is proportional to the system volume. In the two anisotropic limits, therefore, the elementary excitation of Z2 fluxes occurs in a pair of closed loops so as to satisfy both two global constraints as well as the local constraints.

Reconciling estimates of the ratio of heat and salt fluxes at the ice-ocean interface

Science.gov (United States)

Keitzl, T.; Mellado, J. P.; Notz, D.

2016-12-01

The heat exchange between floating ice and the underlying ocean is determined by the interplay of diffusive fluxes directly at the ice-ocean interface and turbulent fluxes away from it. In this study, we examine this interplay through direct numerical simulations of free convection. Our results show that an estimation of the interface flux ratio based on direct measurements of the turbulent fluxes can be difficult because the flux ratio varies with depth. As an alternative, we present a consistent evaluation of the flux ratio based on the total heat and salt fluxes across the boundary layer. This approach allows us to reconcile previous estimates of the ice-ocean interface conditions. We find that the ratio of heat and salt fluxes directly at the interface is 83-100 rather than 33 as determined by previous turbulence measurements in the outer layer. This can cause errors in the estimated ice-ablation rate from field measurements of up to 40% if they are based on the three-equation formulation.

What fraction of the total metal flux into lakes is retained in the sediments

Energy Technology Data Exchange (ETDEWEB)

Nriagu, J O; Wong, H K.T.

1986-12-01

The concentrations of, and deposition rates for, the pollutant metals (Pb, Cu, Zn, Ni, and Cd) and the rare earth elements are reported for the sediments and settling particulates in a large, remote lake (Lake Opeongo) in the Algonquin Provincial Park, Ontario. Using a mass conservation model based on the rare earth elements, it is estimated that only 40 to 60% of pollutant metals getting into the lake is retained by the sediments. The low retention rate may explain the widely reported intrabasin and intra-lake differences in metal contents of sediments and casts some doubt on the use of pollutant metal profiles in retrospective assessment of the human contribution to the metal flux into many lake basins. 22 references.

Measurement of neutrino flux from neutrino-electron elastic scattering

Science.gov (United States)

Park, J.; Aliaga, L.; Altinok, O.; Bellantoni, L.; Bercellie, A.; Betancourt, M.; Bodek, A.; Bravar, A.; Budd, H.; Cai, T.; Carneiro, M. F.; Christy, M. E.; Chvojka, J.; da Motta, H.; Dytman, S. A.; Díaz, G. A.; Eberly, B.; Felix, J.; Fields, L.; Fine, R.; Gago, A. M.; Galindo, R.; Ghosh, A.; Golan, T.; Gran, R.; Harris, D. A.; Higuera, A.; Kleykamp, J.; Kordosky, M.; Le, T.; Maher, E.; Manly, S.; Mann, W. A.; Marshall, C. M.; Martinez Caicedo, D. A.; McFarland, K. S.; McGivern, C. L.; McGowan, A. M.; Messerly, B.; Miller, J.; Mislivec, A.; Morfín, J. G.; Mousseau, J.; Naples, D.; Nelson, J. K.; Norrick, A.; Nuruzzaman; Osta, J.; Paolone, V.; Patrick, C. E.; Perdue, G. N.; Rakotondravohitra, L.; Ramirez, M. A.; Ray, H.; Ren, L.; Rimal, D.; Rodrigues, P. A.; Ruterbories, D.; Schellman, H.; Solano Salinas, C. J.; Tagg, N.; Tice, B. G.; Valencia, E.; Walton, T.; Wolcott, J.; Wospakrik, M.; Zavala, G.; Zhang, D.; Miner ν A Collaboration

2016-06-01

Muon-neutrino elastic scattering on electrons is an observable neutrino process whose cross section is precisely known. Consequently a measurement of this process in an accelerator-based νμ beam can improve the knowledge of the absolute neutrino flux impinging upon the detector; typically this knowledge is limited to ˜10 % due to uncertainties in hadron production and focusing. We have isolated a sample of 135 ±17 neutrino-electron elastic scattering candidates in the segmented scintillator detector of MINERvA, after subtracting backgrounds and correcting for efficiency. We show how this sample can be used to reduce the total uncertainty on the NuMI νμ flux from 9% to 6%. Our measurement provides a flux constraint that is useful to other experiments using the NuMI beam, and this technique is applicable to future neutrino beams operating at multi-GeV energies.

Understanding start-up problems in yeast glycolysis.

Science.gov (United States)

Overal, Gosse B; Teusink, Bas; Bruggeman, Frank J; Hulshof, Josephus; Planqué, Robert

2018-05-01

Yeast glycolysis has been the focus of research for decades, yet a number of dynamical aspects of yeast glycolysis remain poorly understood at present. If nutrients are scarce, yeast will provide its catabolic and energetic needs with other pathways, but the enzymes catalysing upper glycolytic fluxes are still expressed. We conjecture that this overexpression facilitates the rapid transition to glycolysis in case of a sudden increase in nutrient concentration. However, if starved yeast is presented with abundant glucose, it can enter into an imbalanced state where glycolytic intermediates keep accumulating, leading to arrested growth and cell death. The bistability between regularly functioning and imbalanced phenotypes has been shown to depend on redox balance. We shed new light on these phenomena with a mathematical analysis of an ordinary differential equation model, including NADH to account for the redox balance. In order to gain qualitative insight, most of the analysis is parameter-free, i.e., without assigning a numerical value to any of the parameters. The model has a subtle bifurcation at the switch between an inviable equilibrium state and stable flux through glycolysis. This switch occurs if the ratio between the flux through upper glycolysis and ATP consumption rate of the cell exceeds a fixed threshold. If the enzymes of upper glycolysis would be barely expressed, our model predicts that there will be no glycolytic flux, even if external glucose would be at growth-permissable levels. The existence of the imbalanced state can be found for certain parameter conditions independent of the mentioned bifurcation. The parameter-free analysis proved too complex to directly gain insight into the imbalanced states, but the starting point of a branch of imbalanced states can be shown to exist in detail. Moreover, the analysis offers the key ingredients necessary for successful numerical continuation, which highlight the existence of this bistability and the

Fast modeling of flux trapping cascaded explosively driven magnetic flux compression generators.

Science.gov (United States)

Wang, Yuwei; Zhang, Jiande; Chen, Dongqun; Cao, Shengguang; Li, Da; Liu, Chebo

2013-01-01

To predict the performance of flux trapping cascaded flux compression generators, a calculation model based on an equivalent circuit is investigated. The system circuit is analyzed according to its operation characteristics in different steps. Flux conservation coefficients are added to the driving terms of circuit differential equations to account for intrinsic flux losses. To calculate the currents in the circuit by solving the circuit equations, a simple zero-dimensional model is used to calculate the time-varying inductance and dc resistance of the generator. Then a fast computer code is programmed based on this calculation model. As an example, a two-staged flux trapping generator is simulated by using this computer code. Good agreements are achieved by comparing the simulation results with the measurements. Furthermore, it is obvious that this fast calculation model can be easily applied to predict performances of other flux trapping cascaded flux compression generators with complex structures such as conical stator or conical armature sections and so on for design purpose.

On standard forms for transport equations and fluxes: Part 2

International Nuclear Information System (INIS)

Ross, D.W.

1990-03-01

Quasilinear expressions for anomalous particle and energy fluxes arising from electrostatic plasma turbulence in a tokamak are reviewed yet again. Further clarifications are made, and the position taken in a previous report is modified. There, the total energy flux, Q j , and the conductive heat flux, q j , were correctly defined, and the anomalous Q j was correctly calculated. It was shown that the anomalous energy transport can be correctly described by ∇·Q* j , where Q* j = 3/5 Q j , with all remaining source terms such as left-angle p j ∇·Vj} cancelling. Here, a revised discussion is given of the identification of the anomalous conductive flux, q j , in which the distinction between Q j and Q* j is reconsidered. It is shown that there is more than one consistent way to define q j . Transport calculations involving only theoretical electrostatic turbulent fluxes are unaffected by these distinctions since Q j or Q* j , rather than q j , is the quantity naturally calculated in the theory. However, an ambiguity remains in experimental transport analysis if the measured particle flux Γ j = n j V j is to be used in the energy equation. This is because we cannot be sure how properly to treat the source terms p j ∇·V j or { p j ∇·V j }. 17 refs

Estimates of CO2 fluxes over the city of Cape Town, South Africa, through Bayesian inverse modelling

Science.gov (United States)

Nickless, Alecia; Rayner, Peter J.; Engelbrecht, Francois; Brunke, Ernst-Günther; Erni, Birgit; Scholes, Robert J.

2018-04-01

We present a city-scale inversion over Cape Town, South Africa. Measurement sites for atmospheric CO2 concentrations were installed at Robben Island and Hangklip lighthouses, located downwind and upwind of the metropolis. Prior estimates of the fossil fuel fluxes were obtained from a bespoke inventory analysis where emissions were spatially and temporally disaggregated and uncertainty estimates determined by means of error propagation techniques. Net ecosystem exchange (NEE) fluxes from biogenic processes were obtained from the land atmosphere exchange model CABLE (Community Atmosphere Biosphere Land Exchange). Uncertainty estimates were based on the estimates of net primary productivity. CABLE was dynamically coupled to the regional climate model CCAM (Conformal Cubic Atmospheric Model), which provided the climate inputs required to drive the Lagrangian particle dispersion model. The Bayesian inversion framework included a control vector where fossil fuel and NEE fluxes were solved for separately.Due to the large prior uncertainty prescribed to the NEE fluxes, the current inversion framework was unable to adequately distinguish between the fossil fuel and NEE fluxes, but the inversion was able to obtain improved estimates of the total fluxes within pixels and across the domain. The median of the uncertainty reductions of the total weekly flux estimates for the inversion domain of Cape Town was 28 %, but reach as high as 50 %. At the pixel level, uncertainty reductions of the total weekly flux reached up to 98 %, but these large uncertainty reductions were for NEE-dominated pixels. Improved corrections to the fossil fuel fluxes would be possible if the uncertainty around the prior NEE fluxes could be reduced. In order for this inversion framework to be operationalised for monitoring, reporting, and verification (MRV) of emissions from Cape Town, the NEE component of the CO2 budget needs to be better understood. Additional measurements of Δ14C and δ13C isotope

Fast Flux Watch: A mechanism for online detection of fast flux networks

Directory of Open Access Journals (Sweden)

Basheer N. Al-Duwairi

2014-07-01

Full Text Available Fast flux networks represent a special type of botnets that are used to provide highly available web services to a backend server, which usually hosts malicious content. Detection of fast flux networks continues to be a challenging issue because of the similar behavior between these networks and other legitimate infrastructures, such as CDNs and server farms. This paper proposes Fast Flux Watch (FF-Watch, a mechanism for online detection of fast flux agents. FF-Watch is envisioned to exist as a software agent at leaf routers that connect stub networks to the Internet. The core mechanism of FF-Watch is based on the inherent feature of fast flux networks: flux agents within stub networks take the role of relaying client requests to point-of-sale websites of spam campaigns. The main idea of FF-Watch is to correlate incoming TCP connection requests to flux agents within a stub network with outgoing TCP connection requests from the same agents to the point-of-sale website. Theoretical and traffic trace driven analysis shows that the proposed mechanism can be utilized to efficiently detect fast flux agents within a stub network.

Sonar gas flux estimation by bubble insonification: application to methane bubble flux from seep areas in the outer Laptev Sea

Science.gov (United States)

Leifer, Ira; Chernykh, Denis; Shakhova, Natalia; Semiletov, Igor

2017-06-01

Sonar surveys provide an effective mechanism for mapping seabed methane flux emissions, with Arctic submerged permafrost seepage having great potential to significantly affect climate. We created in situ engineered bubble plumes from 40 m depth with fluxes spanning 0.019 to 1.1 L s-1 to derive the in situ calibration curve (Q(σ)). These nonlinear curves related flux (Q) to sonar return (σ) for a multibeam echosounder (MBES) and a single-beam echosounder (SBES) for a range of depths. The analysis demonstrated significant multiple bubble acoustic scattering - precluding the use of a theoretical approach to derive Q(σ) from the product of the bubble σ(r) and the bubble size distribution where r is bubble radius. The bubble plume σ occurrence probability distribution function (Ψ(σ)) with respect to Q found Ψ(σ) for weak σ well described by a power law that likely correlated with small-bubble dispersion and was strongly depth dependent. Ψ(σ) for strong σ was largely depth independent, consistent with bubble plume behavior where large bubbles in a plume remain in a focused core. Ψ(σ) was bimodal for all but the weakest plumes. Q(σ) was applied to sonar observations of natural arctic Laptev Sea seepage after accounting for volumetric change with numerical bubble plume simulations. Simulations addressed different depths and gases between calibration and seep plumes. Total mass fluxes (Qm) were 5.56, 42.73, and 4.88 mmol s-1 for MBES data with good to reasonable agreement (4-37 %) between the SBES and MBES systems. The seepage flux occurrence probability distribution function (Ψ(Q)) was bimodal, with weak Ψ(Q) in each seep area well described by a power law, suggesting primarily minor bubble plumes. The seepage-mapped spatial patterns suggested subsurface geologic control attributing methane fluxes to the current state of subsea permafrost.

Flux flow and flux dynamics in high-Tc superconductors

International Nuclear Information System (INIS)

Bennett, L.H.; Turchinskaya, M.; Swartzendruber, L.J.; Roitburd, A.; Lundy, D.; Ritter, J.; Kaiser, D.L.

1991-01-01

Because high temperature superconductors, including BYCO and BSSCO, are type 2 superconductors with relatively low H(sub c 1) values and high H(sub c 2) values, they will be in a critical state for many of their applications. In the critical state, with the applied field between H(sub c 1) and H(sub c 2), flux lines have penetrated the material and can form a flux lattice and can be pinned by structural defects, chemical inhomogeneities, and impurities. A detailed knowledge of how flux penetrates the material and its behavior under the influence of applied fields and current flow, and the effect of material processing on these properties, is required in order to apply, and to improve the properties of these superconductors. When the applied field is changed rapidly, the time dependence of flux change can be divided into three regions, an initial region which occurs very rapidly, a second region in which the magnetization has a 1n(t) behavior, and a saturation region at very long times. A critical field is defined for depinning, H(sub c,p) as that field at which the hysteresis loop changes from irreversible to reversible. As a function of temperature, it is found that H(sub c,p) is well described by a power law with an exponent between 1.5 and 2.5. The behavior of H(sub c,p) for various materials and its relationship to flux flow and flux dynamics are discussed

Fluxes and concentrations of volatile organic compounds from a South-East Asian tropical rainforest

Directory of Open Access Journals (Sweden)

B. Langford

2010-09-01

Full Text Available As part of the OP3 field study of rainforest atmospheric chemistry, above-canopy fluxes of isoprene, monoterpenes and oxygenated volatile organic compounds were made by virtual disjunct eddy covariance from a South-East Asian tropical rainforest in Malaysia. Approximately 500 hours of flux data were collected over 48 days in April–May and June–July 2008. Isoprene was the dominant non-methane hydrocarbon emitted from the forest, accounting for 80% (as carbon of the measured emission of reactive carbon fluxes. Total monoterpene emissions accounted for 18% of the measured reactive carbon flux. There was no evidence for nocturnal monoterpene emissions and during the day their flux rate was dependent on both light and temperature. The oxygenated compounds, including methanol, acetone and acetaldehyde, contributed less than 2% of the total measured reactive carbon flux. The sum of the VOC fluxes measured represents a 0.4% loss of daytime assimilated carbon by the canopy, but atmospheric chemistry box modelling suggests that most (90% of this reactive carbon is returned back to the canopy by wet and dry deposition following chemical transformation. The emission rates of isoprene and monoterpenes, normalised to 30 °C and 1000 μmol m⁻² s⁻¹ PAR, were 1.6 mg m⁻² h⁻¹ and 0.46mg m⁻² h⁻¹ respectively, which was 4 and 1.8 times lower respectively than the default value for tropical forests in the widely-used MEGAN model of biogenic VOC emissions. This highlights the need for more direct canopy-scale flux measurements of VOCs from the world's tropical forests.

Neutron flux monitor

International Nuclear Information System (INIS)

Oda, Naotaka.

1993-01-01

The device of the present invention greatly saves an analog processing section such as an analog filter and an analog processing circuit. That is, the device of the present invention comprises (1) a neutron flux detection means for detecting neutron fluxed in the reactor, (2) a digital filter means for dividing signals corresponding to the detected neutron fluxes into predetermined frequency band regions, (3) a calculation processing means for applying a calculation processing corresponding to the frequency band regions to the neutron flux detection signals divided by the digital filter means. With such a constitution, since the neutron detection signals are processed by the digital filter means, the accuracy is improved and the change for the property of the filter is facilitated. Further, when a neutron flux level is obtained, a calculation processing corresponding to the frequency band region can be conducted without the analog processing circuit. Accordingly, maintenance and accuracy are improved by greatly decreasing the number of parts. Further, since problems inherent to the analog circuit are solved, neutron fluxes are monitored at high reliability. (I.S.)

Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism.

Science.gov (United States)

Yu, Li; Sun, Yifan; Li, Jingjing; Wang, Yan; Zhu, Yuxing; Shi, Yong; Fan, Xiaojun; Zhou, Jianda; Bao, Ying; Xiao, Jie; Cao, Ke; Cao, Peiguo

2017-08-15

Radiotherapy has been used increasingly to treat primary hepatocellular carcinoma. Clinically, the main cause of radiotherapy failure is cellular radioresistance, conferred via glycolytic metabolism. Our previous study demonstrated that Girdin is upregulated in primary hepatocellular carcinoma and promotes the invasion and metastasis of tumor cells. However, whether Girdin underlies the radio-sensitivity of hepatocellular carcinoma remains unclear. A short hairpin RNA (shRNA) was used to silence CCDC88A (encoding Girdin), and real-time PCR was performed to determine CCDC88A mRNA expression. Then, cell proliferation, colony formation, flow cytometric, scratch, and transwell assays were to examine the influence of Girdin silencing on cellular radiosensitivity. Glycolysis assays were conducted to exam cell glycolysis process. Western blotting was performed to explore the signaling pathway downstream of Girdin. Finally, animal experiments were performed to demonstrate the effect of CCDC88A silencing on the radiosensitivity of hepatoma in vivo. shRNA-induced Girdin silencing suppressed glycolysis and enhanced the radio-sensitivity of hepatic cell lines, HepG2 and Huh-7. Furthermore, silencing of Girdin inhibited the PI3K/AKT/HIF-1α signaling pathway, which is a central regulator of glycolysis. Girdin can regulate glycolysis in hepatocellular carcinoma cells through the PI3K/AKT/HIF-1α signaling pathway, which decreases the sensitivity of tumor cells to radiotherapy.

Quantifying benthic nitrogen fluxes in Puget Sound, Washington: a review of available data

Science.gov (United States)

Sheibley, Richard W.; Paulson, Anthony J.

2014-01-01

Understanding benthic fluxes is important for understanding the fate of materials that settle to the Puget Sound, Washington, seafloor, as well as the impact these fluxes have on the chemical composition and biogeochemical cycles of marine waters. Existing approaches used to measure benthic nitrogen flux in Puget Sound and elsewhere were reviewed and summarized, and factors for considering each approach were evaluated. Factors for selecting an appropriate approach for gathering information about benthic flux include: availability of resources, objectives of projects, and determination of which processes each approach measures. An extensive search of literature was undertaken to summarize known benthic nitrogen fluxes in Puget Sound. A total of 138 individual flux chamber measurements and 38 sets of diffusive fluxes were compiled for this study. Of the diffusive fluxes, 35 new datasets were located, and new flux calculations are presented in this report. About 65 new diffusive flux calculations are provided across all nitrogen species (nitrate, NO3-; nitrite, NO2-; ammonium, NH4+). Data analysis of this newly compiled benthic flux dataset showed that fluxes beneath deep (greater than 50 meters) water tended to be lower than those beneath shallow (less than 50 meters) water. Additionally, variability in flux at the shallow depths was greater, possibly indicating a more dynamic interaction between the benthic and pelagic environments. The overall range of bottom temperatures from studies in the Puget Sound area were small (5–16 degrees Celsius), and only NH4+ flux showed any pattern with temperature. For NH4+, flux values and variability increased at greater than about 12 degrees Celsius. Collection of additional study site metadata about environmental factors (bottom temperature, depth, sediment porosity, sediment type, and sediment organic matter) will help with development of a broader regional understanding benthic nitrogen flux in the Puget Sound.

Effect of skim milk treated with high hydrostatic pressure on permeate flux and fouling during ultrafiltration.

Science.gov (United States)

Leu, Mathilde; Marciniak, Alice; Chamberland, Julien; Pouliot, Yves; Bazinet, Laurent; Doyen, Alain

2017-09-01

Ultrafiltration (UF) is largely used in the dairy industry to generate milk and whey protein concentrate for standardization of milk or production of dairy ingredients. Recently, it was demonstrated that high hydrostatic pressure (HHP) extended the shelf life of milk and improved rennet coagulation and cheese yield. Pressurization also modified casein micelle size distribution and promoted aggregation of whey proteins. These changes are likely to affect UF performance. Consequently, this study determined the effect of skim milk pressurization (300 and 600 MPa, 5 min) on UF performance in terms of permeate flux decline and fouling. The effect of HHP on milk proteins was first studied and UF was performed in total recycle mode at different transmembrane pressures to determine optimal UF operational parameters and to evaluate the effect of pressurization on critical and limiting fluxes. Ultrafiltration was also performed in concentration mode at a transmembrane pressure of 345 kPa for 130 or 140 min to evaluate the decline of permeate flux and to determine fouling resistances. It was observed that average casein micelle size decreased by 32 and 38%, whereas β-lactoglobulin denaturation reached 30 and 70% at 300 and 600 MPa, respectively. These results were directly related to UF performance because initial permeate fluxes in total recycle mode decreased by 25% at 300 and 600 MPa compared with nonpressurized milk, critical flux, and limiting flux, which were lower during UF of milk treated with HHP. During UF in concentration mode, initial permeate fluxes were 30% lower at 300 and 600 MPa compared with the control, but the total flux decline was higher for nonpressurized milk (62%) compared with pressure-treated milk (30%). Fouling resistances were similar, whatever the treatment, except at 600 MPa where irreversible fouling was higher. Characterization of the fouling layer showed that caseins and β-lactoglobulin were mainly involved in membrane fouling after UF of

TRANSHEX, 2-D Thermal Neutron Flux Distribution from Epithermal Flux in Hexagonal Geometry

International Nuclear Information System (INIS)

Patrakka, E.

1994-01-01

1 - Description of program or function: TRANSHEX is a multigroup integral transport program that determines the thermal scalar flux distribution arising from a known epithermal flux in two- dimensional hexagonal geometry. 2 - Method of solution: The program solves the isotropic collision probability equations for a region-averaged scalar flux by an iterative method. Either a successive over-relaxation or an inner-outer iteration technique is applied. Flat flux collision probabilities between trigonal space regions with white boundary condition are utilized. The effect of epithermal flux is taken into consideration as a slowing-down source that is calculated for a given spatial distribution and 1/E energy dependence of the epithermal flux

Nonoscillatory shock capturing scheme using flux limited dissipation

International Nuclear Information System (INIS)

Jameson, A.

1985-01-01

A method for modifying the third order dissipative terms by the introduction of flux limiters is proposed. The first order dissipative terms can then be eliminated entirely, and in the case of a scalar conservation law the scheme is converted into a total variation diminishing scheme provided that an appropriate value is chosen for the dissipative coefficient. Particular attention is given to: (1) the treatment of the scalar conservation law; (2) the treatment of the Euler equations for inviscid compressible flow; (3) the boundary conditions; and (4) multistage time stepping and multigrid schemes. Numerical results for transonic flows suggest that a central difference scheme augmented by flux limited dissipative terms can lead to an effective nonoscillatory shock capturing method. 20 references

Radon flux measurement methodologies

International Nuclear Information System (INIS)

Nielson, K.K.; Rogers, V.C.

1984-01-01

Five methods for measuring radon fluxes are evaluated: the accumulator can, a small charcoal sampler, a large-area charcoal sampler, the ''Big Louie'' charcoal sampler, and the charcoal tent sampler. An experimental comparison of the five flux measurement techniques was also conducted. Excellent agreement was obtained between the measured radon fluxes and fluxes predicted from radium and emanation measurements

Cross-ecosystem impacts of stream pollution reduce resource and contaminant flux to riparian food webs

Science.gov (United States)

Kraus, Johanna M.; Schmidt, Travis S.; Walters, David; Wanty, Richard B.; Zuellig, Robert E.; Wolf, Ruth E.

2014-01-01

The effects of aquatic contaminants are propagated across ecosystem boundaries by aquatic insects that export resources and contaminants to terrestrial food webs; however, the mechanisms driving these effects are poorly understood. We examined how emergence, contaminant concentration, and total contaminant flux by adult aquatic insects changed over a gradient of bioavailable metals in streams and how these changes affected riparian web-building spiders. Insect emergence decreased 97% over the metal gradient, whereas metal concentrations in adult insects changed relatively little. As a result, total metal exported by insects (flux) was lowest at the most contaminated streams, declining 96% among sites. Spiders were affected by the decrease in prey biomass, but not by metal exposure or metal flux to land in aquatic prey. Aquatic insects are increasingly thought to increase exposure of terrestrial consumers to aquatic contaminants, but stream metals reduce contaminant flux to riparian consumers by strongly impacting the resource linkage. Our results demonstrate the importance of understanding the contaminant-specific effects of aquatic pollutants on adult insect emergence and contaminant accumulation in adults to predict impacts on terrestrial food webs.

Bacterial Flux by Net Precipitation from the Phyllosphere to the Forest Floor.

Science.gov (United States)

Pound, P.; Van Stan, J. T., II; Moore, L. D.; Bittar, T.

2016-12-01

Transport pathways of microbes between ecosystem spheres (atmosphere, phyllosphere, and pedosphere) represent major fluxes in nutrient cycles and have the potential to significantly affect microbial ecological processes. We quantified a previously unexamined microbial flux from the phyllosphere to the pedosphere during rainfall and found it to be substantial. Net rainfall bacterial fluxes for throughfall and stemflow were quantified using flow cytometry and a quantitative Polymerase Chain Reaction (qPCR) assay for a Quercus virginiana (Mill., southern live oak) forest with heavy epiphyte cover of Tillandsia usneoides (L., Spanish moss) and Pleopeltis polypodiodes (L., resurrection fern) in coastal Georgia (Southeast USA). Total net precipitation flux of bacteria was 15 quadrillion cells year-1 ha-1, which (assuming a bacterial cell mass of 1 pg) is approximately 15 kg of bacterial biomass supply per year. Stemflow generation was low in this stand (rarely exceeded 10 L storm-1) yet still delivered half the annual net precipitation flux due to high bacterial concentration. The role of this previously unquantified bacterial flux in the forest floor has also been under studied, yet it may be significant by contributing functional community members (if living) or labile lysates (if dead).

Inter-comparison of Flux-Gradient and Relaxed Eddy Accumulation Methods for Measuring Ammonia Flux Above a Corn Canopy in Central Illinois, USA

Science.gov (United States)

Nelson, A. J.; Koloutsou-Vakakis, S.; Rood, M. J.; Lichiheb, N.; Heuer, M.; Myles, L.

2017-12-01

Ammonia (NH3) is a precursor to fine particulate matter (PM) in the ambient atmosphere. Agricultural activities represent over 80% of anthropogenic emissions of NH3 in the United States. The use of nitrogen-based fertilizers contribute > 50% of total NH3 emissions in central Illinois. The U.S. EPA Science Advisory Board has called for improved methods to measure, model, and report atmospheric NH3 concentrations and emissions from agriculture. High uncertainties in the temporal and spatial distribution of NH3 emissions contribute to poor performance of air quality models in predicting ambient PM concentrations. This study reports and compares NH­3 flux measurements of differing temporal resolution obtained with two methods: relaxed eddy accumulation (REA) and flux-gradient (FG). REA and FG systems were operated concurrently above a corn canopy at the University of Illinois at Urbana-Champaign (UIUC) Energy Biosciences Institute (EBI) Energy Farm during the 2014 corn-growing season. The REA system operated during daytime, providing average fluxes over four-hour sampling intervals, where time resolution was limited by detection limit of denuders. The FG system employed a cavity ring-down spectrometer, and was operated continuously, reporting 30 min flux averages. A flux-footprint evaluation was used for quality control, resulting in 1,178 qualified FG measurements, 82 of which were coincident with REA measurements. Similar emission trends were observed with both systems, with peak NH3 emission observed one week after fertilization. For all coincident samples, mean NH3 flux was 205 ± 300 ng-N-m2s-1 and 110 ± 256 ng-N-m2s-1 as measured with REA and FG, respectively, where positive flux indicates emission. This is the first reported inter-comparison of REA and FG methods as used for quantifying NH3 fluxes from cropland. Preliminary analysis indicates the improved temporal resolution and continuous sampling enabled by FG allow for the identification of emission pulses

Seasonality and variability of coccolithophore fluxes in response to diverse oceanographic regimes in the Bay of Bengal: Sediment trap results

Digital Repository Service at National Institute of Oceanography (India)

Mergulhao, L.P.; Guptha, M.V.S.; Unger, D.; Murty, V.S.N.

carbonate and diatom opal fluxes continued to increase. Tanaka and Kawahata (2001) reported that the calculated coccolith- CaCO3 fluxes ranged between 6.8 and 49.6 mg m-2day-1 and contributed 23.3% of total CaCO3 flux in the west Caroline Basin...

Hawking fluxes and anomalies in rotating regular black holes with a time-delay

International Nuclear Information System (INIS)

Takeuchi, Shingo

2016-01-01

Based on the anomaly cancellation method we compute the Hawking fluxes (the Hawking thermal flux and the total flux of energy-momentum tensor) from a four-dimensional rotating regular black hole with a time-delay. To this purpose, in the three metrics proposed in [1], we try to perform the dimensional reduction in which the anomaly cancellation method is feasible at the near-horizon region in a general scalar field theory. As a result we can demonstrate that the dimensional reduction is possible in two of those metrics. Hence we perform the anomaly cancellation method and compute the Hawking fluxes in those two metrics. Our Hawking fluxes involve three effects: (1) quantum gravity effect regularizing the core of the black holes, (2) rotation of the black hole, (3) time-delay. Further in this paper toward the metric in which the dimensional could not be performed, we argue that it would be some problematic metric, and mention its cause. The Hawking fluxes we compute in this study could be considered to correspond to more realistic Hawking fluxes. Further what Hawking fluxes can be obtained from the anomaly cancellation method would be interesting in terms of the relation between a consistency of quantum field theories and black hole thermodynamics. (paper)

Cyclic variation of heat flux on spark plug; Tenka plug bu no netsuryusoku hendo no sokutei

Energy Technology Data Exchange (ETDEWEB)

Ishii, K.; Sasaki, T.; Urata, Y. [Honda Motor Co. Ltd., Tokyo (Japan); Kagawa, J.; Matsutani, W. [NGK Spark Plug Co. Ltd., Nagoya (Japan)

1998-02-25

This paper examines the relationship between the magnitude of the heat flux to the spark plug ground electrode, averaged over an 80` crank angle (CA) of early compression stroke, and the initial burning rate, defined as the CA at which 5% of mass is burned. The heat flux was measured by a thin-film thermocouple with the hot junction on the surface of ground electrode. The results demonstrate that faster initial burning rate correlated well with increasing heat flux from the spark plug to the mixture. The difference in the magnitude and direction of the heat flux is associated with the amount of residual gas concentration and thus the results show the effect of residual gas concentration. The cycle-averaged heat flux from the hot junction is 0.367 MW/m{sup 2}, corresponding to a total heat flow of 20 W from the total surface area of ground electrode. This value is about an order of magnitude larger than that previously reported in the literature for locations away from the spark plug, e. g. at the cylinder wall. 11 refs., 9 figs., 1 tab.

Flux pinning and flux flow studies in superconductors using flux flow noise techniques. Progress report, April 1, 1976--December 17, 1976

International Nuclear Information System (INIS)

Joiner, W.C.H.

1976-12-01

Measurements of flux flow noise power spectra have been combined with critical current measurements and measurements of current-voltage characteristics to study flux flow and local pinning interactions effective during flux flow. A model of flux flow noise generation in the presence of local pinning interactions is developed and applied to situations where pinning is dominated by: (1) grain boundaries, (2) normal metal precipitates in a superconducting matrix, (3) gross deformation producing a critical current peak effect, and (4) surface grooves imposed on a sample surface. In the case of pinning caused by normal metal precipitates in a superconducting matrix, unusual training and hysterisis effects are observed in the flux flow characteristics. The greater sensitivity of noise spectra, as compared with bulk critical current measurements, in obtaining a detailed picture of flux flow is emphasized

On dryout heat flux and pressure drop of a submerged inductively heated bed flow from below

International Nuclear Information System (INIS)

Tsai, F.F.; Catton, I.

1983-01-01

An experimental investigation of dryout heat flux in a saturated porous medal with forced flow from below has been conducted using methanol as a coolant. The mass flux varied from 0 to 0.557 kg/m 2 sec. Particle sizes were 590-790 μm, 1.6 mm, 3.2 mm, and 4.8 mm. The dryout heat flux increases as the mass flux increases, and asymptotically goes to the total evaporation energy of the inlet flow. The pressure drop across the bed changed very rapidly near the dryout point due to the formation of dry zone

Critical heat flux predictions for the Sandia Annular Core Research Reactor

International Nuclear Information System (INIS)

Rao, D.V.; El-Genk, M.S.

1994-08-01

This study provides best estimate predictions of the Critical Heat Flux (CHF) and the Critical Heat Flux Ratio (CHFR) to support the proposed upgrade of the Annual Core Research Reactor (ACRR) at Sandia National Laboratories (SNL) from its present value of 2 MWt to 4 MWt. These predictions are based on the University of New Mexico (UNM) - CHF correlation, originally developed for uniformly heated vertical annuli. The UNM-CHF correlation is applicable to low-flow and low-pressure conditions, which are typical of those in the ACRR. The three hypotheses that examined the effect of the nonuniform axial heat flux distribution in the ACRR core are (1) the local conditions hypotheses, (2) the total power hypothesis, and (3) the global conditions hypothesis. These hypotheses, in conjunction with the UNM-CHF correlation, are used to estimate the CHF and CHFR in the ACRR. Because the total power hypothesis predictions of power per rod at CHF are approximately 15%-20% lower than those corresponding to saturation exit conditions, it can be concluded that the total power hypothesis considerably underestimates the CHF for nonuniformly heated geometries. This conclusion is in agreement with previous experimental results. The global conditions hypothesis, which is more conservative and more accurate of the other two, provides the most reliable predictions of CHF/CHFR for the ACRR. The global conditions hypothesis predictions of CHFR varied between 2.1 and 3.9, with the higher value corresponding to the lower water inlet temperature of 20 degrees C

Measurement of the cosmic ray and neutrino-induced muon flux at the Sudbury neutrino observatory

OpenAIRE

Aharmim, B; Peeters, S J M; SNO Collaboration,

2009-01-01

Results are reported on the measurement of the atmospheric neutrino-induced muon flux at a depth of 2 kilometers below the Earth's surface from 1229 days of operation of the Sudbury Neutrino Observatory (SNO). By measuring the flux of through-going muons as a function of zenith angle, the SNO experiment can distinguish between the oscillated and un-oscillated portion of the neutrino flux. A total of 514 muon-like events are measured between $-1 \\le \\cos{\\theta}_{\\rm zenith} \\le 0.4$ in a tota...

A road map to solar neutrino fluxe, neutrino oscillation parameters, and tests for new physics

CERN Document Server

Bahcall, J N; Bahcall, John N.; Peña-Garay, Carlos

2003-01-01

We analyze all available solar and related reactor neutrino experiments, as well as simulated future ^7Be, p-p, pep, and ^8B solar neutrino experiments. We treat all solar neutrino fluxes as free parameters subject to the condition that the total luminosity represented by the neutrinos equals the observed solar luminosity (the `luminosity constraint'). Existing experiments show that the p-p solar neutrino flux is 1.01 + - 0.02 (1 sigma) times the flux predicted by the BP00 standard solar model; the ^7Be neutrino flux is 0.97^{+0.28}_{-0.54} the predicted flux; and the ^8B flux is 1.01 + - 0.06 the predicted flux. The oscillation parameters are: Delta m^2 = 7.3^{+0.4}_{-0.6} 10^{-5} eV^2 and tan^2 theta_{12} = 0.42^{+0.08}_{-0.06}. We evaluate how accurate future experiments must be to determine more precisely neutrino oscillation parameters and solar neutrino fluxes, and to elucidate the transition from vacuum-dominated to matter-dominated oscillations. A future ^7Be nu-e scattering experiment accurate to + -...

Flux cutting in superconductors

International Nuclear Information System (INIS)

Campbell, A M

2011-01-01

This paper describes experiments and theories of flux cutting in superconductors. The use of the flux line picture in free space is discussed. In superconductors cutting can either be by means of flux at an angle to other layers of flux, as in longitudinal current experiments, or due to shearing of the vortex lattice as in grain boundaries in YBCO. Experiments on longitudinal currents can be interpreted in terms of flux rings penetrating axial lines. More physical models of flux cutting are discussed but all predict much larger flux cutting forces than are observed. Also, cutting is occurring at angles between vortices of about one millidegree which is hard to explain. The double critical state model and its developments are discussed in relation to experiments on crossed and rotating fields. A new experiment suggested by Clem gives more direct information. It shows that an elliptical yield surface of the critical state works well, but none of the theoretical proposals for determining the direction of E are universally applicable. It appears that, as soon as any flux flow takes place, cutting also occurs. The conclusion is that new theories are required. (perspective)

Study of the effect of the energy spectrum and of the total flux on the damage produced by neutrons in solids; Contribution a l'etude de l'influence du spectre et du flux integre sur les dommages crees par les neutrons dans les solides

Energy Technology Data Exchange (ETDEWEB)

Dulieu, P C [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

1967-01-01

In the first part are studied the general relationships between the physical effects produced by neutrons in solids, and the total flux and neutron energy spectrum; some examples are given. The second part, describes the application to a silicon damage detector whose principle is to use the damage produced in a PIN Junction for measuring the neutron flux (intermediate and fast) received by the detector. Chapter I is devoted to the experimental determination of the energy given to the atoms by a primary in the silicon. The results and conclusions drawn from this determination make it possible to consider, in chapter II, the calculation of the detectors response characteristics.Chapter III deals with the measurement of the detectors response function and it is noted that good agreement is obtained between the calculation and experimental results. The whole of the second part constitutes a test of the methods presented in the first part. (author) [French] Dans la premiere partie, nous etudions les relations generales qui lient les effets physiques engendres par les neutrons dans les solides au flux integre et au spectre des neutrons et nous donnons des exemples d'utilisation. La deuxieme partie est une application au detecteur de dommages en silicium, dont le principe est d'utiliser les dommages crees dans une jonction PIN pour mesurer les flux de neutrons (intermediaires et rapides) recus par le detecteur. Le chapitre I est consacre a la determination experimentale de l'energie cedee aux atomes par un primaire dans le silicium. Les resultats et les conclusions que l'on peut tirer de cette determination permettent d'aborder, au chapitre II, le calcul de la fonction de reponse du detecteur. Le chapitre III porte sur la mesure de la fonction de reponse du detecteur et on constate qu'il y a un bon accord entre le calcul et l'experience. L'ensemble de la deuxieme partie constitue un test des methodes exposees dans la premiere partie. (auteur)

Tree Species with Photosynthetic Stems Have Greater Nighttime Sap Flux

Directory of Open Access Journals (Sweden)

Xia Chen

2018-01-01

Full Text Available An increasing body of evidence has shown that nighttime sap flux occurs in most plants, but the physiological implications and regulatory mechanism are poorly known. The significance of corticular photosynthesis has received much attention during the last decade, however, the knowledge of the relationship between corticular photosynthesis and nocturnal stem sap flow is limited at present. In this study, we divided seven tree species into two groups according to different photosynthetic capabilities: trees of species with (Castanopsis hystrix, Michelia macclurei, Eucalyptus citriodora, and Eucalyptus grandis × urophylla and without (Castanopsis fissa, Schima superba, and Acacia auriculiformis photosynthetic stems, and the sap flux (Js and chlorophyll fluorescence parameters for these species were measured. One-way ANOVA analysis showed that the Fv/Fm (Maximum photochemical quantum yield of PSII and ΦPSII (effective photochemical quantum yield of PSII values were lower in non-photosynthetic stem species compared to photosynthetic stem species. The linear regression analysis showed that Js,d (daytime sap flux and Js,n (nighttime sap flux of non-photosynthetic stem species was 87.7 and 60.9% of the stem photosynthetic species. Furthermore, for a given daytime transpiration water loss, total nighttime sap flux was higher in species with photosynthetic stems (SlopeSMA = 2.680 than in non-photosynthetic stems species (SlopeSMA = 1.943. These results mean that stem corticular photosynthesis has a possible effect on the nighttime water flow, highlighting the important eco-physiological relationship between nighttime sap flux and corticular photosynthesis.

Tree Species with Photosynthetic Stems Have Greater Nighttime Sap Flux

Science.gov (United States)

Chen, Xia; Gao, Jianguo; Zhao, Ping; McCarthy, Heather R.; Zhu, Liwei; Ni, Guangyan; Ouyang, Lei

2018-01-01

An increasing body of evidence has shown that nighttime sap flux occurs in most plants, but the physiological implications and regulatory mechanism are poorly known. The significance of corticular photosynthesis has received much attention during the last decade, however, the knowledge of the relationship between corticular photosynthesis and nocturnal stem sap flow is limited at present. In this study, we divided seven tree species into two groups according to different photosynthetic capabilities: trees of species with (Castanopsis hystrix, Michelia macclurei, Eucalyptus citriodora, and Eucalyptus grandis × urophylla) and without (Castanopsis fissa, Schima superba, and Acacia auriculiformis) photosynthetic stems, and the sap flux (Js) and chlorophyll fluorescence parameters for these species were measured. One-way ANOVA analysis showed that the Fv/Fm (Maximum photochemical quantum yield of PSII) and ΦPSII (effective photochemical quantum yield of PSII) values were lower in non-photosynthetic stem species compared to photosynthetic stem species. The linear regression analysis showed that Js,d (daytime sap flux) and Js,n (nighttime sap flux) of non-photosynthetic stem species was 87.7 and 60.9% of the stem photosynthetic species. Furthermore, for a given daytime transpiration water loss, total nighttime sap flux was higher in species with photosynthetic stems (SlopeSMA = 2.680) than in non-photosynthetic stems species (SlopeSMA = 1.943). These results mean that stem corticular photosynthesis has a possible effect on the nighttime water flow, highlighting the important eco-physiological relationship between nighttime sap flux and corticular photosynthesis. PMID:29416547

Are Polar Field Magnetic Flux Concentrations Responsible for Missing Interplanetary Flux?

Science.gov (United States)

Linker, Jon A.; Downs, C.; Mikic, Z.; Riley, P.; Henney, C. J.; Arge, C. N.

2012-05-01

Magnetohydrodynamic (MHD) simulations are now routinely used to produce models of the solar corona and inner heliosphere for specific time periods. These models typically use magnetic maps of the photospheric magnetic field built up over a solar rotation, available from a number of ground-based and space-based solar observatories. The line-of-sight field at the Sun's poles is poorly observed, and the polar fields in these maps are filled with a variety of interpolation/extrapolation techniques. These models have been found to frequently underestimate the interplanetary magnetic flux (Riley et al., 2012, in press, Stevens et al., 2012, in press) near the minimum part of the cycle unless mitigating correction factors are applied. Hinode SOT observations indicate that strong concentrations of magnetic flux may be present at the poles (Tsuneta et al. 2008). The ADAPT flux evolution model (Arge et al. 2010) also predicts the appearance of such concentrations. In this paper, we explore the possibility that these flux concentrations may account for a significant amount of magnetic flux and alleviate discrepancies in interplanetary magnetic flux predictions. Research supported by AFOSR, NASA, and NSF.

The contribution of various types of settling particles to the flux of organic carbon in the Gulf of St. Lawrence

Science.gov (United States)

Romero-Ibarra, Nancy; Silverberg, Norman

2011-10-01

The contents of 31 samples from free-drifting sediment traps deployed in the Gulf of St. Lawrence (GSL) were analyzed for the individual contribution of the different types of particles encountered to the total particulate organic carbon (POC) flux. Two trap models were used in 1993-1994: small traps at 50 m depth and large traps at 50 and 150 m. Total POC fluxes averaged 42 mg C m -2 d -1 for the more reliable large trap and 149 mg C m -2 d -1 for the small trap. The POC fluxes were attributed to different classes of particles based upon microscopically determined particle dimensions and carbon/volume algorithms available in the literature. Fecal pellets, followed by phytoplankton, were the major attributable components, with important contributions by microzooplankton, particularly during the summer of 1994. The mean fluxes for pellets (6 and 60 mg C m -2 d -1, for the large and small traps, respectively) and phytoplankton (3.2 and 42.9 mg C m -2 d -1) were in the range of those encountered in other areas of moderate primary productivity. Mean zooplankton carbon fluxes (1.8 and 8.5 mg C m -2 d -1, respectively), however, reflect higher than average zooplankton abundances in the GSL. The C fluxes of specific algal groups confirmed the existence of three trophic regimes previously identified from water column studies and numeric cell fluxes: (1) a period when diatoms were dominant during the spring, (2) a longer interval, which was dominated by dinoflagellates at most others times of the year, and (3) a period of transition during summer. Carbon of animal origin dominated the attributable flux, including an important fraction associated with heterotrophic dinoflagellates. The contribution of marine snow to the total flux (estimated as the difference between the total POC flux and the sum of the attributed components) frequently amounted to more than 60%. The true importance of marine snow remains uncertain, however, because the errors associated with each of the

Aerosol fluxes and particle growth above managed grassland

Directory of Open Access Journals (Sweden)

E. Nemitz

2009-08-01

Full Text Available Particle deposition velocities (11–3000 nm diameter measured above grassland by eddy covariance during the EU GRAMINAE experiment in June 2000 averaged 0.24 and 0.03 mm s⁻¹ to long (0.75 m and short (0.07 m grass, respectively. After fertilisation with 108 kg N ha⁻¹ as calcium ammonium nitrate, sustained apparent upward fluxes of particles were observed. Analysis of concentrations and fluxes of potential precursor gases, including NH₃, HNO₃, HCl and selected VOCs, shows that condensation of HNO₃ and NH₃ on the surface of existing particles is responsible for this effect. A novel approach is developed to derive particle growth rates at the field scale, from a combination of measurements of vertical fluxes and particle size-distributions. For the first 9 days after fertilization, growth rates of 11 nm particles of 7.04 nm hr⁻¹ and 1.68 nm hr⁻¹ were derived for day and night-time conditions, respectively. This implies total NH₄NO₃ production rates of 1.11 and 0.44 μg m⁻³ h⁻¹, respectively. The effect translates into a small error in measured ammonia fluxes (0.06% day, 0.56% night and a large error in NH₄⁺ and NO₃⁻ aerosol fluxes of 3.6% and 10%, respectively. By converting rapidly exchanged NH₃ and HNO₃ into slowly depositing NH₄NO₃, the reaction modifies the total N budget, though this effect is small (<1% for the 10 days following fertilization, as NH₃ emission dominates the net flux. It is estimated that 3.8% of the fertilizer N was volatilised as NH₃, of which 0.05% re-condensed to form NH₄NO₃ particles within the lowest 2 m of the surface layer. This surface induced process would at least scale up to a global NH₄NO₃ formation of ca. 0.21 kt N yr

Ozone Flux Measurement and Modelling on Leaf/Shoot and Canopy Scale

Directory of Open Access Journals (Sweden)

Ludger Grünhage

calculated by SVAT models often based on the energy balance of the soil-vegetation-atmosphere system and on the big-leaf concept. This latter assumes the canopy as equivalent to a single leaf having a leaf area equal to the total area of all the plant’s leaves and lying at a certain height above the ground. The complexity of SVAT models ranges from one-dimensional to three-dimensional models. The most used are one-dimensional models in single-layer, dual-source or multi-layer version. The main uncertainties in flux modelling are currently associated to the estimation of the non-stomatal flux component and to the up-scaling process from leaf to canopy and stand level. For the latter a separate representation of sunlit and shaded leaves is recommended.

The flow distribution in the parallel tubes of the cavity receiver under variable heat flux

International Nuclear Information System (INIS)

Hao, Yun; Wang, Yueshe; Hu, Tian

2016-01-01

Highlights: • An experimental loop is built to find the flow distribution in the parallel tubes. • With the concentration of heat flux, two-phase flow makes distribution more uneven. • The total flow rate is chosen appropriately for a wider heat flux distribution. • A suitable system pressure is essential for the optimization of flow distribution. - Abstract: As an optical component of tower solar thermal power station, the heliostat mirror reflects sunlight to one point of the heated surface in the solar cavity receiver, called as one-point focusing system. The radiation heat flux concentrated in the cavity receiver is always non-uniform temporally and spatially, which may lead to extremely local over-heat on the receiver evaporation panels. In this paper, an electrical heated evaporating experimental loop, including five parallel vertical tubes, is set up to evaluate the hydrodynamic characteristics of evaporation panels in a solar cavity receiver under various non-uniform heat flux. The influence of the heat flux concentration ratio, total flow rate, and system pressure on the flow distribution of parallel tubes is discussed. It is found that the flow distribution becomes significantly worse with the increase of heat flux and concentration ratio; and as the system pressure decreased, the flow distribution is improved. It is extremely important to obtain these interesting findings for the safe and stable operation of solar cavity receiver, and can also provide valuable references for the design and optimization of operating parameters solar tower power station system.

Distribution and flux of 226Ra and 228Ra in the Amazon River estuary

International Nuclear Information System (INIS)

Key, R.M.; Sarmiento, J.L.; Stallard, R.F.; Moore, W.S.

1985-01-01

Measurements of 226 Ra and 228 Ra in the Amazon River estuary show that desorption from riverborne suspended particulate matter in the estuary increases the riverine flux of both isotopes to the ocean by a factor of approximately 5 over the flux attributable to radium dissolved in the river water alone. The total Amazon flux supplies approximately 0.20% of the 226 Ra and approximately 2.6% of the 228 Ra standing crops in the near-surface Atlantic (0-200 m). Diffusive flux from estuarine and shelf sediments and desorption from resuspended sediments in the region of the estuary approximately double the estuarine 226 Ra concentration and quadruple the estuarine 228 Ra concentration above that caused by the dissolved and desorbed river components alone

Seasonal variation, flux estimation, and source analysis of dissolved emerging organic contaminants in the Yangtze Estuary, China.

Science.gov (United States)

Zhao, Heng; Cao, Zhen; Liu, Xue; Zhan, Yi; Zhang, Jing; Xiao, Xi; Yang, Yi; Zhou, Junliang; Xu, Jiang

2017-12-15

The occurrence and seasonal variation of 24 dissolved emerging organic contaminants in the Yangtze Estuary were studied, including 12 non-antibiotic pharmaceuticals, seven sulfonamides, two macrolides and three chloramphenicols. Sulfadiazine, erythromycin, thiamphenicol and paracetamol were the primary contaminants in sulfonamides, macrolides, chloramphenicols and non-antibiotic pharmaceutical groups, respectively. Compared to the concentrations at Datong, chloramphenicols at Xuliujing were significantly higher in autumn and winter, while macrolides were lower in spring. Based on the flux estimation, approximately 37.1 tons of sulfonamides, 17.4 tons of macrolides, 79.2 tons of chloramphenicols and 14.1 tons of non-antibiotic pharmaceuticals were discharged into the Yangtze Estuary from June 2013 to May 2014. However, the total flux from the Huangpu River only represented 5% of the total. The pharmaceutical sources were speculated on by analyzing the seasonal variations in pharmaceutical concentrations and fluxes at various sites. Both environmental and social factors might affect the fluxes. Copyright © 2017 Elsevier Ltd. All rights reserved.

MHD Simulations of the Eruption of Coronal Flux Ropes under Coronal Streamers

Energy Technology Data Exchange (ETDEWEB)

Fan, Yuhong, E-mail: yfan@ucar.edu [High Altitude Observatory, National Center for Atmospheric Research, 3080 Center Green Drive, Boulder, CO 80301 (United States)

2017-07-20

Using three-dimensional magnetohydrodynamic (MHD) simulations, we investigate the eruption of coronal flux ropes underlying coronal streamers and the development of a prominence eruption. We initialize a quasi-steady solution of a coronal helmet streamer, into which we impose at the lower boundary the slow emergence of a part of a twisted magnetic torus. As a result, a quasi-equilibrium flux rope is built up under the streamer. With varying streamer sizes and different lengths and total twists of the flux rope that emerges, we found different scenarios for the evolution from quasi-equilibrium to eruption. In the cases with a broad streamer, the flux rope remains well confined until there is sufficient twist such that it first develops the kink instability and evolves through a sequence of kinked, confined states with increasing height until it eventually develops a “hernia-like” ejective eruption. For significantly twisted flux ropes, prominence condensations form in the dips of the twisted field lines due to runaway radiative cooling. Once formed, the prominence-carrying field becomes significantly non-force-free due to the weight of the prominence, despite having low plasma β . As the flux rope erupts, the prominence erupts, showing substantial draining along the legs of the erupting flux rope. The prominence may not show a kinked morphology even though the flux rope becomes kinked. On the other hand, in the case with a narrow streamer, the flux rope with less than one wind of twist can erupt via the onset of the torus instability.

Fractional flux excitations and flux creep in a superconducting film

International Nuclear Information System (INIS)

Lyuksyutov, I.F.

1995-01-01

We consider the transport properties of a modulated superconducting film in a magnetic field parallel to the film. Modulation can be either intrinsic, due to the layered structure of the high-T c superconductors, or artificial, e.g. due to thickness modulation. This system has an infinite set ( >) of pinned phases. In the pinned phase the excitation of flux loops with a fractional number of flux quanta by the applied current j results in flux creep with a generated voltage V ∝ exp[-jo/j[. (orig.)

Analytical characterization of selective benthic flux components in estuarine and coastal waters

Science.gov (United States)

King, Jeffrey N.

2011-01-01

Benthic flux is the rate of flow across the bed of a water body, per unit area of bed. It is forced by component mechanisms, which interact. For example, pressure gradients across the bed, forced by tide, surface gravity waves, density gradients, bed–current interaction, turbulence, and terrestrial hydraulic gradients, drive an advective benthic flux of water and constituents between estuarine and coastal waters, and surficial aquifers. Other mechanisms also force benthic flux, such as chemical gradients, bioturbation, and dispersion. A suite of component mechanisms force a total benthic flux at any given location, where each member of the suite contributes a component benthic flux. Currently, the types and characteristics of component interactions are not fully understood. For example, components may interact linearly or nonlinearly, and the interaction may be constructive or destructive. Benthic flux is a surface water–groundwater interaction process. Its discharge component to a marine water body is referred to, in some literature, as submarine groundwater discharge. Benthic flux is important in characterizing water and constituent budgets of estuarine and coastal systems. Analytical models to characterize selective benthic flux components are reviewed. Specifically, these mechanisms are for the component associated with the groundwater tidal prism, and forced by surface gravity wave setup, surface gravity waves on a plane bed, and the terrestrial hydraulic gradient. Analytical models are applied to the Indian River Lagoon, Florida; Great South Bay, New York; and the South Atlantic Bight in South Carolina and portions of North Carolina.

Integrated passive flux measurement in groundwater: design and performance of iFLUX samplers

Science.gov (United States)

Verreydt, Goedele; Razaei, Meisam; Meire, Patrick; Van Keer, Ilse; Bronders, Jan; Seuntjens, Piet

2017-04-01

The monitoring and management of soil and groundwater is a challenge. Current methods for the determination of movement or flux of pollution in groundwater use no direct measurements but only simulations based on concentration measurements and Darcy velocity estimations. This entails large uncertainties which cause remediation failures and higher costs for contaminated site owners. On top of that, the lack of useful data makes it difficult to get approval for a risk-based management approach which completely avoids costly remedial actions. The iFLUX technology is a key development of Dr. Goedele Verreydt at the University of Antwerp and VITO. It is supported by the passive flux measurement technology as invented by Prof. Mike Annable and his team at the University of Florida. The iFLUX technology includes an in situ measurement device for capturing dynamic groundwater quality and quantity, the iFLUX sampler, and an associated interpretation and visualization method. The iFLUX sampler is a modular passive sampler that provides simultaneous in situ point determinations of a time-averaged target compound mass flux and water flux. The sampler is typically installed in a monitoring well where it intercepts the groundwater flow and captures the compounds of interest. The sampler consists of permeable cartridges which are each packed with a specific sorbent matrix. The sorbent matrix of the water flux cartridge is impregnated with known amounts of water soluble resident tracers. These tracers are leached from the matrix at rates proportional to the groundwater flux. The measurements of the contaminants and the remaining resident tracer are used to determine groundwater and target compound fluxes. Exposure times range from 1 week to 6 months, depending on the expected concentration and groundwater flow velocity. The iFLUX sampler technology has been validated and tested at several field projects. Currently, 4 cartridges are tested and available: 1 waterflux cartridge to

A flux footprint analysis to understand ecosystem fluxes in an intensively managed landscape

Science.gov (United States)

Hernandez Rodriguez, L. C.; Goodwell, A. E.; Kumar, P.

2017-12-01

Flux tower studies in agricultural sites have mainly been done at plot scale, where the footprint of the instruments is small such that the data reveals the behaviour of the nearby crop on which the study is focused. In the Midwestern United States, the agricultural ecosystem and its associated drainage, evapotranspiration, and nutrient dynamics are dominant influences on interactions between the soil, land, and atmosphere. In this study, we address large-scale ecohydrologic fluxes and states in an intensively managed landscape based on data from a 25m high eddy covariance flux tower. We show the calculated upwind distance and flux footprint for a flux tower located in Central Illinois as part of the Intensively Managed Landscapes Critical Zone Observatory (IMLCZO). In addition, we calculate the daily energy balance during the summer of 2016 from the flux tower measurements and compare with the modelled energy balance from a representative corn crop located in the flux tower footprint using the Multi-Layer Canopy model, MLCan. The changes in flux footprint over the course of hours, days, and the growing season have significant implications for the measured fluxes of carbon and energy at the flux tower. We use MLCan to simulate these fluxes under land covers of corn and soybeans. Our results demonstrate how the instrument heights impact the footprint of the captured eddy covariance fluxes, and we explore the implication for hydrological analysis. The convective turbulent atmosphere during the daytime shows a wide footprint of more than 10 km2, which reaches 3km length for the 90% contribution, where buoyancy is the dominant mechanism driving turbulence. In contrast, the stable atmosphere during the night-time shows a narrower footprint that goes beyond 8km2 and grows in the direction of the prevalent wind, which exceeds 4 km in length. This study improves our understanding of agricultural ecosystem behaviour in terms of the magnitude and variability of fluxes and

Structures of interplanetary magnetic flux ropes and comparison with their solar sources

Energy Technology Data Exchange (ETDEWEB)

Hu, Qiang [Department of Space Science/CSPAR, University of Alabama in Huntsville, Huntsville, AL 35805 (United States); Qiu, Jiong [Department of Physics, Montana State University, Bozeman, MT 59717-3840 (United States); Dasgupta, B.; Khare, A.; Webb, G. M., E-mail: qh0001@uah.edu, E-mail: qiu@physics.montana.edu [Center for Space Plasma and Aeronomic Research (CSPAR), University of Alabama in Huntsville, Huntsville, AL 35805 (United States)

2014-09-20

Whether a magnetic flux rope is pre-existing or formed in situ in the Sun's atmosphere, there is little doubt that magnetic reconnection is essential to release the flux rope during its ejection. During this process, the question remains: how does magnetic reconnection change the flux-rope structure? In this work, we continue with the original study of Qiu et al. by using a larger sample of flare-coronal mass ejection (CME)-interplanetary CME (ICME) events to compare properties of ICME/magnetic cloud (MC) flux ropes measured at 1 AU and properties of associated solar progenitors including flares, filaments, and CMEs. In particular, the magnetic field-line twist distribution within interplanetary magnetic flux ropes is systematically derived and examined. Our analysis shows that, similar to what was found before, for most of these events, the amount of twisted flux per AU in MCs is comparable with the total reconnection flux on the Sun, and the sign of the MC helicity is consistent with the sign of the helicity of the solar source region judged from the geometry of post-flare loops. Remarkably, we find that about half of the 18 magnetic flux ropes, most of them associated with erupting filaments, have a nearly uniform and relatively low twist distribution from the axis to the edge, and the majority of the other flux ropes exhibit very high twist near the axis, up to ≳ 5 turns per AU, which decreases toward the edge. The flux ropes are therefore not linearly force-free. We also conduct detailed case studies showing the contrast of two events with distinct twist distribution in MCs as well as different flare and dimming characteristics in solar source regions, and discuss how reconnection geometry reflected in flare morphology may be related to the structure of the flux rope formed on the Sun.

Expected total counts for the Self-Interrogation Neutron Resonance Densitometry measurements of spent nuclear fuel

Energy Technology Data Exchange (ETDEWEB)

Rossa, Riccardo [Belgian nuclear research centre SCK.CEN (Belgium); Universite Libre de Bruxelles (Belgium); Borella, Alessandro; Van der Meer, Klaas [Belgian nuclear research centre SCK.CEN. Boeretang 200, 2400 Mol (Belgium); Labeau, Pierre-Etienne; Pauly, Nicolas [Universite Libre de Bruxelles. Av. F. D. Roosevelt 50, B1050 Brussels (Belgium)

2015-07-01

The Self-Interrogation Neutron Resonance Densitometry (SINRD) is a passive neutron technique that aims at a direct quantification of {sup 239}Pu in spent fuel assemblies by measuring the attenuation of the neutron flux in the energy region close to the 0.3 eV resonance of {sup 239}Pu. The {sup 239}Pu mass is estimated by calculating the SINRD signature, that is the ratio between the neutron counts in the fast energy region and around the 0.3 eV resonance region. The SINRD measurement approach in this study consisted in introducing a small neutron detector in the central guide tube of a PWR 17x17 fuel assembly. In order to measure the neutron flux in the energy regions defined in the SINRD signature, different detector types were used. The response of a bare {sup 238}U fission chamber is considered for the determination of the fast neutron flux, while other thermal-epithermal detectors wrapped in neutron absorbers are envisaged to measure the neutron flux around the resonance region. This paper provides an estimation of the total neutron counts that can be achieved with the detector types proposed for the SINRD measurement. In the first section a set of detectors are evaluated in terms of total neutron counts and sensitivity to the {sup 239}Pu content, in order to identify the optimal measurement configuration for each detector type. Then a study is performed to increase the total neutron counts by increasing the detector size. The study shows that the highest total neutron counts are achieved by using either {sup 3}He or {sup 10}B proportional counters because of the high neutron efficiency of these detectors. However, the calculations indicate that the biggest contribution to the measurement uncertainty is due to the measurement of the fast neutron flux. Finally, similar sensitivity to the {sup 239}Pu content is obtained by using the different detector types for the measurement of the neutron flux close to the resonance region. Therefore, the total neutron counts

Automated flux chamber for investigating gas flux at water-air interfaces.

Science.gov (United States)

Duc, Nguyen Thanh; Silverstein, Samuel; Lundmark, Lars; Reyier, Henrik; Crill, Patrick; Bastviken, David

2013-01-15

Aquatic ecosystems are major sources of greenhouse gases (GHG). Representative measurements of GHG fluxes from aquatic ecosystems to the atmosphere are vital for quantitative understanding of relationships between biogeochemistry and climate. Fluxes occur at high temporal variability at diel or longer scales, which are not captured by traditional short-term deployments (often in the order of 30 min) of floating flux chambers. High temporal frequency measurements are necessary but also extremely labor intensive if manual flux chamber based methods are used. Therefore, we designed an inexpensive and easily mobile automated flux chamber (AFC) for extended deployments. The AFC was designed to measure in situ accumulation of gas in the chamber and also to collect gas samples in an array of sample bottles for subsequent analysis in the laboratory, providing two independent ways of CH(4) concentration measurements. We here present the AFC design and function together with data from initial laboratory tests and from a field deployment.

Summer fluxes of atmospheric greenhouse gases N{sub 2}O, CH{sub 4} and CO{sub 2} from mangrove soil in South China

Energy Technology Data Exchange (ETDEWEB)

Chen, G.C. [Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR (China); Tam, N.F.Y., E-mail: bhntam@cityu.edu.hk [Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR (China); Ye, Y. [State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian (China)

2010-06-01

The atmospheric fluxes of N{sub 2}O, CH{sub 4} and CO{sub 2} from the soil in four mangrove swamps in Shenzhen and Hong Kong, South China were investigated in the summer of 2008. The fluxes ranged from 0.14 to 23.83 {mu}mol m{sup -2} h{sup -1}, 11.9 to 5168.6 {mu}mol m{sup -2} h{sup -1} and 0.69 to 20.56 mmol m{sup -2} h{sup -1} for N{sub 2}O, CH{sub 4} and CO{sub 2}, respectively. Futian mangrove swamp in Shenzhen had the highest greenhouse gas fluxes, followed by Mai Po mangrove in Hong Kong. Sha Kong Tsuen and Yung Shue O mangroves in Hong Kong had similar, low fluxes. The differences in both N{sub 2}O and CH{sub 4} fluxes among different tidal positions, the landward, seaward and bare mudflat, in each swamp were insignificant. The N{sub 2}O and CO{sub 2} fluxes were positively correlated with the soil organic carbon, total nitrogen, total phosphate, total iron and NH{sub 4}{sup +}-N contents, as well as the soil porosity. However, only soil NH{sub 4}{sup +}-N concentration had significant effects on CH{sub 4} fluxes.

Poynting flux measurements on a satellite: A diagnostic tool for space research

International Nuclear Information System (INIS)

Kelley, M.C.; Knudsen, D.J.; Vickery, J.F.

1991-01-01

The first satellite observations of the total field-aligned component of the quasi-dc Poynting flux are presented for two passes over the polar region, one in the noon sector and one in the afternoon. The energy input due to electron precipitation is also presented. In the noon pass the downward Poynting flux in the auroral oval was comparable to the kinetic energy input rate. The peak electromagnetic energy input rate of 6 ergs/(cm 2 s) equaled the peak particle input while the integrated electromagnetic value along the trajectory was 60% that of the particles. In the afternoon pass the peak electromagnetic energy input was also about 6 ergs/(cm 2 s), but the peak particle energy was 6 times this value. The average electromagnetic input was 10% of the particle input for the pass. In this study, the authors can measure the Poynting flux only over a limited range of scale sizes; thus the contribution to the total energy budget in the polar cap cannot be determined. Both passes show small regions characterized by upward Poynting flux suggesting a neutral wind dynamo. There is also evidence during part of the noontime pass that the external generator acted in opposition to an existing wind field since the Poynting flux was greater than the estimate of Joule heating from the electric field measurement alone (i.e., from Σ p E 2 ). In the course of deriving Poynting's theorem for the geophysical case they also present a proof that ground magnetometer systems respond primarily to the Hall current which does not depend upon geometric cancellation between the field generated by Pedersen and field-aligned currents

Engineering strategies aimed at control of acidification rate of lactic acid bacteria

DEFF Research Database (Denmark)

Martinussen, Jan; Solem, Christian; Holm, Anders Koefoed

2013-01-01

The ability of lactic acid bacteria to produce lactic acid from various sugars plays an important role in food fermentations. Lactic acid is derived from pyruvate, the end product of glycolysis and thus a fast lactic acid production rate requires a high glycolytic flux. In addition to lactic acid......, alternative end products - ethanol, acetic acid and formic acid - are formed by many species. The central role of glycolysis in lactic acid bacteria has provoked numerous studies aiming at identifying potential bottleneck(s) since knowledge about flux control could be important not only for optimizing food...

Application of a Weighted Regression Model for Reporting Nutrient and Sediment Concentrations, Fluxes, and Trends in Concentration and Flux for the Chesapeake Bay Nontidal Water-Quality Monitoring Network, Results Through Water Year 2012

Science.gov (United States)

Chanat, Jeffrey G.; Moyer, Douglas L.; Blomquist, Joel D.; Hyer, Kenneth E.; Langland, Michael J.

2016-01-13

In the Chesapeake Bay watershed, estimated fluxes of nutrients and sediment from the bay’s nontidal tributaries into the estuary are the foundation of decision making to meet reductions prescribed by the Chesapeake Bay Total Maximum Daily Load (TMDL) and are often the basis for refining scientific understanding of the watershed-scale processes that influence the delivery of these constituents to the bay. Two regression-based flux and trend estimation models, ESTIMATOR and Weighted Regressions on Time, Discharge, and Season (WRTDS), were compared using data from 80 watersheds in the Chesapeake Bay Nontidal Water-Quality Monitoring Network (CBNTN). The watersheds range in size from 62 to 70,189 square kilometers and record lengths range from 6 to 28 years. ESTIMATOR is a constant-parameter model that estimates trends only in concentration; WRTDS uses variable parameters estimated with weighted regression, and estimates trends in both concentration and flux. WRTDS had greater explanatory power than ESTIMATOR, with the greatest degree of improvement evident for records longer than 25 years (30 stations; improvement in median model R2= 0.06 for total nitrogen, 0.08 for total phosphorus, and 0.05 for sediment) and the least degree of improvement for records of less than 10 years, for which the two models performed nearly equally. Flux bias statistics were comparable or lower (more favorable) for WRTDS for any record length; for 30 stations with records longer than 25 years, the greatest degree of improvement was evident for sediment (decrease of 0.17 in median statistic) and total phosphorus (decrease of 0.05). The overall between-station pattern in concentration trend direction and magnitude for all constituents was roughly similar for both models. A detailed case study revealed that trends in concentration estimated by WRTDS can operationally be viewed as a less-constrained equivalent to trends in concentration estimated by ESTIMATOR. Estimates of annual mean flow

Estimating sediment and caesium-137 fluxes in the Ribble Estuary through time-series airborne remote sensing

International Nuclear Information System (INIS)

Wakefield, R.; Tyler, A.N.; McDonald, P.; Atkin, P.A.; Gleizon, P.; Gilvear, D.

2011-01-01

High spatial and temporal resolution airborne imagery were acquired for the Ribble Estuary, North West England in 1997 and 2003, to assess the application of time-series airborne remote sensing to quantify total suspended sediment and radionuclide fluxes during a flood and ebb tide sequence. Concomitant measurements of suspended particulate matter (SPM) and water column turbidity were obtained during the time-series image acquisition for the flood and ebb tide sequence on the 17th July 2003 to verify the assumption of a vertically well mixed estuary and thus justifying the vertical extrapolation of spatially integrated estimate of surface SPM. The 137 Cs activity concentrations were calculated from a relatively stable relationship between SPM and 137 Cs for the Ribble Estuary. Total estuary wide budgets of sediment and 137 Cs were obtained by combining the image-derived estimates of surface SPM and 137 Cs with estimates of water volume from a two-dimensional hydrodynamic model (VERSE) developed for the Ribble Estuary. These indicate that around 10,000 tonnes of sediment and 2.72 GBq of 137 Cs were deposited over the tidal sequence monitored in July 2003. This compared favourably with bed height elevation change estimated from field work. An uncertainty analysis on the total sediment and 137 Cs flux yielded a total budget of the order of 40% on the final estimate. The results represent a novel approach to providing a spatially integrated estimate of the total net sediment and radionuclide flux in an intertidal environment over a flood and ebb tide sequence. - Research highlights: → This paper provides a rare insight into the next flux of sediment and associated radionuclide loading into an estuary over and ebb and flood tide sequence. → The paper uses high temporal resolution airborne imagery coupled with concomitant sampling to convert total suspended sediment flux to 137 Cs loading. → For the estuary and date studied, it is estimate that an 10,000 tonnes of

Oxygen in the Southern Ocean From Argo Floats: Determination of Processes Driving Air-Sea Fluxes

Science.gov (United States)

Bushinsky, Seth M.; Gray, Alison R.; Johnson, Kenneth S.; Sarmiento, Jorge L.

2017-11-01

The Southern Ocean is of outsized significance to the global oxygen and carbon cycles with relatively poor measurement coverage due to harsh winters and seasonal ice cover. In this study, we use recent advances in the parameterization of air-sea oxygen fluxes to analyze 9 years of oxygen data from a recalibrated Argo oxygen data set and from air-calibrated oxygen floats deployed as part of the Southern Ocean Carbon and Climate Observations and Modeling (SOCCOM) project. From this combined data set of 150 floats, we find a total Southern Ocean oxygen sink of -183 ± 80 Tmol yr-1 (positive to the atmosphere), greater than prior estimates. The uptake occurs primarily in the Polar-Frontal Antarctic Zone (PAZ, -94 ± 30 Tmol O2 yr-1) and Seasonal Ice Zone (SIZ, -111 ± 9.3 Tmol O2 yr-1). This flux is driven by wintertime ventilation, with a large portion of the flux in the SIZ passing through regions with fractional sea ice. The Subtropical Zone (STZ) is seasonally driven by thermal fluxes and exhibits a net outgassing of 47 ± 29 Tmol O2 yr-1 that is likely driven by biological production. The Subantarctic Zone (SAZ) uptake is -25 ± 12 Tmol O2 yr-1. Total oxygen fluxes were separated into a thermal and nonthermal component. The nonthermal flux is correlated with net primary production and mixed layer depth in the STZ, SAZ, and PAZ, but not in the SIZ where seasonal sea ice slows the air-sea gas flux response to the entrainment of deep, low-oxygen waters.

Effect of gas sparging on flux enhancement and phytochemical properties of clarified pineapple juice by microfiltration

KAUST Repository

Laorko, Aporn

2011-08-01

Membrane fouling is a major obstacle in the application of microfiltration. Several techniques have been proposed to enhance the permeate flux during microfiltration. Gas sparging is a hydrodynamic method for improving the performance of the membrane process. In this study, a 0.2 μm hollow fiber microfiltration membrane was used to study the effect of cross flow velocity (CFV) and gas injection factor () on the critical and limiting flux during microfiltration of pineapple juice. In addition, the phytochemical properties of clarified juice were investigated. In the absence of gas sparging, the critical and limiting flux increased as the CFV or shear stress number increased. The use of gas sparging led to a remarkable improvement in both the critical and limiting flux but it was more effective at the lower CFV (1.5 m s-1), compared to those at higher CFV (2.0 and 2.5 m s-1). When the gas injection factor was applied at 0.15, 0.25 and 0.35 with a CFV of 1.5 m s -1, the enhancement of 55.6%, 75.5% and 128.2% was achieved for critical flux, while 65.8%, 69.7% and 95.2% was achieved for limiting flux, respectively. The results also indicated that the use of gas sparging was an effective method to reduce reversible fouling and external irreversible fouling rather than internal irreversible fouling. In addition, the CFV and gas sparging did not affect pH, total soluble solids, colour, total phenolic content and the antioxidant property of the clarified juice. The l-ascorbic acid and total vitamin C were significantly decreased when the higher CFV and high gas injection factor were applied. The results also indicated that the use of gas sparging with low CFV was beneficial for flux enhancement while most of the phytochemical properties of the clarified juice was preserved. © 2011 Elsevier B.V. All rights reserved.

Physics of magnetic flux ropes

Science.gov (United States)

Russell, C. T.; Priest, E. R.; Lee, L. C.

The present work encompasses papers on the structure, waves, and instabilities of magnetic flux ropes (MFRs), photospheric flux tubes (PFTs), the structure and heating of coronal loops, solar prominences, coronal mass ejections and magnetic clouds, flux ropes in planetary ionospheres, the magnetopause, magnetospheric field-aligned currents and flux tubes, and the magnetotail. Attention is given to the equilibrium of MFRs, resistive instability, magnetic reconnection and turbulence in current sheets, dynamical effects and energy transport in intense flux tubes, waves in solar PFTs, twisted flux ropes in the solar corona, an electrodynamical model of solar flares, filament cooling and condensation in a sheared magnetic field, the magnetopause, the generation of twisted MFRs during magnetic reconnection, ionospheric flux ropes above the South Pole, substorms and MFR structures, evidence for flux ropes in the earth magnetotail, and MFRs in 3D MHD simulations.

Meromorphic flux compactification

Energy Technology Data Exchange (ETDEWEB)

Damian, Cesar [Departamento de Ingeniería Mecánica, Universidad de Guanajuato,Carretera Salamanca-Valle de Santiago Km 3.5+1.8 Comunidad de Palo Blanco,Salamanca (Mexico); Loaiza-Brito, Oscar [Departamento de Física, Universidad de Guanajuato,Loma del Bosque No. 103 Col. Lomas del Campestre C.P 37150 León, Guanajuato (Mexico)

2017-04-26

We present exact solutions of four-dimensional Einstein’s equations related to Minkoswki vacuum constructed from Type IIB string theory with non-trivial fluxes. Following https://www.doi.org/10.1007/JHEP02(2015)187; https://www.doi.org/10.1007/JHEP02(2015)188 we study a non-trivial flux compactification on a fibered product by a four-dimensional torus and a two-dimensional sphere punctured by 5- and 7-branes. By considering only 3-form fluxes and the dilaton, as functions on the internal sphere coordinates, we show that these solutions correspond to a family of supersymmetric solutions constructed by the use of G-theory. Meromorphicity on functions constructed in terms of fluxes and warping factors guarantees that flux and 5-brane contributions to the scalar curvature vanish while fulfilling stringent constraints as tadpole cancelation and Bianchi identities. Different Einstein’s solutions are shown to be related by U-dualities. We present three supersymmetric non-trivial Minkowski vacuum solutions and compute the corresponding soft terms. We also construct a non-supersymmetric solution and study its stability.

Meromorphic flux compactification

International Nuclear Information System (INIS)

Damian, Cesar; Loaiza-Brito, Oscar

2017-01-01

We present exact solutions of four-dimensional Einstein’s equations related to Minkoswki vacuum constructed from Type IIB string theory with non-trivial fluxes. Following https://www.doi.org/10.1007/JHEP02(2015)187; https://www.doi.org/10.1007/JHEP02(2015)188 we study a non-trivial flux compactification on a fibered product by a four-dimensional torus and a two-dimensional sphere punctured by 5- and 7-branes. By considering only 3-form fluxes and the dilaton, as functions on the internal sphere coordinates, we show that these solutions correspond to a family of supersymmetric solutions constructed by the use of G-theory. Meromorphicity on functions constructed in terms of fluxes and warping factors guarantees that flux and 5-brane contributions to the scalar curvature vanish while fulfilling stringent constraints as tadpole cancelation and Bianchi identities. Different Einstein’s solutions are shown to be related by U-dualities. We present three supersymmetric non-trivial Minkowski vacuum solutions and compute the corresponding soft terms. We also construct a non-supersymmetric solution and study its stability.

Autoimmunity against a glycolytic enzyme as a possible cause for persistent symptoms in Lyme disease.

Science.gov (United States)

Maccallini, Paolo; Bonin, Serena; Trevisan, Giusto

2018-01-01

Some patients with a history of Borrelia burgdorferi infection develop a chronic symptomatology characterized by cognitive deficits, fatigue, and pain, despite antibiotic treatment. The pathogenic mechanism that underlines this condition, referred to as post-treatment Lyme disease syndrome (PTLDS), is currently unknown. A debate exists about whether PTLDS is due to persistent infection or to post-infectious damages in the immune system and the nervous system. We present the case of a patient with evidence of exposure to Borrelia burgdorferi sl and a long history of debilitating fatigue, cognitive abnormalities and autonomic nervous system issues. The patient had a positive Western blot for anti-basal ganglia antibodies, and the autoantigen has been identified as γ enolase, the neuron-specific isoenzyme of the glycolytic enzyme enolase. Assuming Borrelia own surface exposed enolase as the source of this autoantibody, through a mechanism of molecular mimicry, and given the absence of sera reactivity to α enolase, a bioinformatical analysis was carried out to identify a possible cross-reactive conformational B cell epitope, shared by Borrelia enolase and γ enolase, but not by α enolase. Taken that evidence, we hypothesize that this autoantibody interferes with glycolysis in neuronal cells, as the physiological basis for chronic symptoms in at least some cases of PTLDS. Studies investigating on the anti-γ enolase and anti-Borrelia enolase antibodies in PTLDS are needed to confirm our hypotheses. Copyright © 2017 Elsevier Ltd. All rights reserved.

Higher order Cambell techniques for neutron flux measurement. Pt. 1

International Nuclear Information System (INIS)

Lux, I.; Baranyai, A.

1982-01-01

An exact mathematical description of arbitrary high order Campbell techniques for measuring particle fluxes is given. The nth order Campbell technique assumes the measurement of the moments of the outcoming voltage up to the nth one. A simple relation is derived among the various moments of the total measured voltage and of the detector signal caused by one incident particle. It is proven that in the monoparticle case combination of the measured moments up to the order n provides an expression proportional to the particle flux and to the nth moment of the detector signal. Generalization to several different particles is given and it is shown that if the flux of the particle causing the largest detector signal is measured with a relative error epsilon in the dc method and the error is due to the signals of other particles, then in the nth order campbelling the error will be of order epsilonsup(n). The effect of a random background on the measured voltage is also investigated and it is established that the nth order campbelling supresses the noise according to the nth power of the relative amplitude of the noise to the signal. The results concerning constant fluxes are generalized to time dependent particle fluxes and a method assuming a Fourier transform of the measured quantities is proposed for their determination. (orig.)

Differential effects of safflower oil versus fish oil feeding on insulin-stimulated glycogen synthesis, glycolysis, and pyruvate dehydrogenase flux in skeletal muscle: a 13C nuclear magnetic resonance study.

Science.gov (United States)

Jucker, B M; Cline, G W; Barucci, N; Shulman, G I

1999-01-01

To examine the effects of safflower oil versus fish oil feeding on in vivo intramuscular glucose metabolism and relative pyruvate dehydrogenase (PDH) versus tricarboxylic acid (TCA) cycle flux, rats were pair-fed on diets consisting of 1) 59% safflower oil, 2) 59% menhaden fish oil, or 3) 59% carbohydrate (control) in calories. Rates of glycolysis and glycogen synthesis were assessed by monitoring [1-(13)C]glucose label incorporation into [1-(13)C]glycogen, [3-(13)C]lactate, and [3-(13)C]alanine in the hindlimb of awake rats via 13C nuclear magnetic resonance (NMR) spectroscopy during a euglycemic (approximately 6 mmol/l) hyperinsulinemic (approximately 180 microU/ml) clamp. A steady-state isotopic analysis of lactate, alanine, and glutamate was used to determine the relative PDH versus TCA cycle flux present in muscle under these conditions. The safflower oil-fed rats were insulin resistant compared with control and fish oil-fed rats, as reflected by a markedly reduced glucose infusion rate (Ginf) during the clamp (21.4 +/- 2.3 vs. 31.6 +/- 2.8 and 31.7 +/- 1.9 mg x kg(-1) x min(-1) in safflower oil versus control and fish oil groups, respectively, P safflower oil group was associated with a lower rate of glycolysis (21.7 +/- 2.2 nmol x g(-1) x min(-1)) versus control (62.1 +/- 10.3 nmol x g(-1) x min(-1), P safflower oil, fish oil, and control, respectively) was detected. The intramuscular triglyceride (TG) content was increased in the safflower oil group (7.3 +/- 0.8 micromol/g) compared with the control group (5.2 +/- 0.8 micromol/g, P safflower oil (43 +/- 8%) versus the control (73 +/- 8%, P safflower oil feeding was a consequence of reduced glycolytic flux associated with an increase in relative free fatty acid/ketone oxidation versus TCA cycle flux, whereas fish oil feeding did not alter glucose metabolism and may in part be protective of insulin-stimulated glucose disposal by limiting intramuscular TG deposition.

Micrometeorological flux measurements of aerosol and gases above Beijing

Science.gov (United States)

Nemitz, Eiko; Langford, Ben; Mullinger, Neil; Cowan, Nicholas; Coyle, Mhairi; Acton, William Joe; Lee, James; Fu, Pingqing

2017-04-01

Air pollution is estimated to cause 1.6 million premature deaths in China every year and in the winter 2016/17 Beijing had to issue health alerts and put in place ad hoc limitations on industrial and vehicular activity. Much of this pollution is attributed to emissions from industrial processes and in particular coal combustion. By contrast, the diffuse pollutant sources within the city are less well understood. This includes, e.g., emissions from the Beijing traffic fleet, the sewage system, food preparation, solid fuel combustion in the streets and small industrial processes. Within the framework of a major UK-Chinese collaboration to study air pollution and its impact on human health in Beijing, we therefore measured fluxes of a large range of pollutants from a height of 102 m on the 325 m meteorological tower at the Institute of Atmospheric Physics. Several instruments were mounted at 102 m: fluxes of CO2 and H2O were measured with an infrared gas analyser (LiCOR 7500) and fluxes of ozone with a combination of a relative fast-response ozone analyser (ROFI) and a 2B absolute O3 instrument. Total particle number fluxes were measured with a condensation particle counter (TSI CPC 3785), and size-segregated fluxes over the size range 0.06 to 20 μm with a combination of an optical Ultrafine High Sensitivity Aerosol Spectrometer (UHSAS) and an Aerodynamic Particle Sizer Spectrometer (TSI APS3321). Ammonia (NH3) fluxes were measured for the first time above the urban environment using an Aerodyne compact quantum cascade laser (QCL). In addition, composition resolved aerosol fluxes were measured with an Aerodyne Aerosol Mass Spectrometer (HR-ToF-AMS), operated in a measurement container at the bottom of the tower, which subsampled from a 120 m long copper tube (15 mm OD). The analysis so far suggests that, due to often low wind speeds, fluxes were at times de-coupled from the surface. Fluxes normalised by CO2, a tracer for the amount of fossil fuel consumed, should be

Vertical nitrogen flux from the oceanic photic zone by diel migrant zooplankton and nekton

Science.gov (United States)

Longhurst, Alan R.; Glen Harrison, W.

1988-06-01

Where the photic zone is a biological steady-state, the downward flux of organic material across the pycnocline to the interior of the ocean is thought to be balanced by upward turbulent flux of inorganic nitrogen across the nutricline. This model ignores a significant downward dissolved nitrogen flux caused by the diel vertical migration of interzonal zooplankton and nekton that feed in the photic zone at night and excrete nitrogenous compounds at depth by day. In the oligotrophic ocean this flux can be equivalent to the flux of particulate organic nitrogen from the photic zone in the form of faecal pellets and organic flocculates. Where nitrogen is the limiting plant nutrient, and the flux by diel migration of interzonal plankton is significant compared to other nitrogen exports from the photic zone, there must be an upward revision of previous estimates for the ratio of new to total primary production in the photic zone if a nutrient balance is to be maintained. This upward revision is of the order 5-100% depending on the oceanographic regime.

Preliminary estimation of Vulcano of CO2 budget and continuous monitoring of summit soil CO2 flux

OpenAIRE

Inguaggiato, S.; Mazot, A.; Diliberto, I. S.; Rouwet, D.; Vita, F.; Capasso, G.; Bobrowski, N.; Inguaggiato, C.; Grassa, F.

2008-01-01

Total CO2 output from fumaroles, soil gases, bubbling and water dissolved gases were estimated at Vulcano Island, Italy. The fumaroles output has been estimated from SO2 plume flux, while soil flux emission has been carried out through 730 CO2 fluxes measured on the island surface, performed by means of accumulation chamber method. Vulcano Island, located in the Aeolian Archipelago, is an active volcano that has been in state of solphataric activity, since the last eru...

Influence of the late winter bloom on migrant zooplankton metabolism and its implications on export fluxes

Science.gov (United States)

Putzeys, S.; Yebra, L.; Almeida, C.; Bécognée, P.; Hernández-León, S.

2011-12-01

Studies on carbon active fluxes due to diel migrants are scarce and critical for carbon flux models and biogeochemical estimates. We studied the temporal variability and vertical distribution of biomass, indices of feeding and respiration of the zooplanktonic community north off the Canary Islands during the end of the late winter bloom, in order to assess vertical carbon fluxes in this area. Biomass distribution during the day presented two dense layers of organisms at 0-200 m and around 500 m, whereas at night, most of the biomass concentrated in the epipelagic layer. The gut pigment flux (0.05-0.18 mgC·m - 2 ·d - 1 ) represented 0.22% of the estimated passive export flux (POC flux) while potential ingestion represented 3.91% of the POC (1.24-3.40 mgC·m - 2 ·d - 1 ). The active respiratory flux (0.50-1.36 mgC·m - 2 ·d - 1 ) was only 1.57% of the POC flux. The total carbon flux mediated by diel migrants (respiration plus potential ingestion) ranged between 3.37 and 9.22% of the POC flux; which is three-fold higher than calculating ingestion fluxes from gut pigments. Our results suggest that the fluxes by diel migrants play a small role in the downward flux of carbon in the open ocean during the post-bloom period.

Simulation of boiling flow in evaporator of separate type heat pipe with low heat flux

International Nuclear Information System (INIS)

Kuang, Y.W.; Wang, Wen; Zhuan, Rui; Yi, C.C.

2015-01-01

Highlights: • A boiling flow model in a separate type heat pipe with 65 mm diameter tube. • Nucleate boiling is the dominant mechanism in large pipes at low mass and heat flux. • The two-phase heat transfer coefficient is less sensitive to the total mass flux. - Abstract: The separate type heat pipe heat exchanger is considered to be a potential selection for developing passive cooling spent fuel pool – for the passive pressurized water reactor. This paper simulates the boiling flow behavior in the evaporator of separate type heat pipe, consisting of a bundle of tubes of inner diameter 65 mm. It displays two-phase characteristic in the evaporation section of the heat pipe working in low heat flux. In this study, the two-phase flow model in the evaporation section of the separate type heat pipe is presented. The volume of fluid (VOF) model is used to consider the interaction between the ammonia gas and liquid. The flow patterns and flow behaviors are studied and the agitated bubbly flow, churn bubbly flow are obtained, the slug bubble is likely to break into churn slug or churn froth flow. In addition, study on the heat transfer coefficients indicates that the nucleate boiling is the dominant mechanism in large pipes at low mass and heat flux, with the heat transfer coefficient being less sensitive to the total mass flux

Understanding alternative fluxes/effluxes through comparative metabolic pathway analysis of phylum actinobacteria using a simplified approach.

Science.gov (United States)

Verma, Mansi; Lal, Devi; Saxena, Anjali; Anand, Shailly; Kaur, Jasvinder; Kaur, Jaspreet; Lal, Rup

2013-12-01

Actinobacteria are known for their diverse metabolism and physiology. Some are dreadful human pathogens whereas some constitute the natural flora for human gut. Therefore, the understanding of metabolic pathways is a key feature for targeting the pathogenic bacteria without disturbing the symbiotic ones. A big challenge faced today is multiple drug resistance by Mycobacterium and other pathogens that utilize alternative fluxes/effluxes. With the availability of genome sequence, it is now feasible to conduct the comparative in silico analysis. Here we present a simplified approach to compare metabolic pathways so that the species specific enzyme may be traced and engineered for future therapeutics. The analyses of four key carbohydrate metabolic pathways, i.e., glycolysis, pyruvate metabolism, tri carboxylic acid cycle and pentose phosphate pathway suggest the presence of alternative fluxes. It was found that the upper pathway of glycolysis was highly variable in the actinobacterial genomes whereas lower glycolytic pathway was highly conserved. Likewise, pentose phosphate pathway was well conserved in contradiction to TCA cycle, which was found to be incomplete in majority of actinobacteria. The clustering based on presence and absence of genes of these metabolic pathways clearly revealed that members of different genera shared identical pathways and, therefore, provided an easy method to identify the metabolic similarities/differences between pathogenic and symbiotic organisms. The analyses could identify isoenzymes and some key enzymes that were found to be missing in some pathogenic actinobacteria. The present work defines a simple approach to explore the effluxes in four metabolic pathways within the phylum actinobacteria. The analysis clearly reflects that actinobacteria exhibit diverse routes for metabolizing substrates. The pathway comparison can help in finding the enzymes that can be used as drug targets for pathogens without effecting symbiotic organisms

Fate modeling of mercury species and fluxes estimation in an urban river

International Nuclear Information System (INIS)

Tong, Yindong; Zhang, Wei; Chen, Cen; Chen, Long; Wang, Wentao; Hu, Xindi; Wang, Huanhuan; Hu, Dan; Ou, Langbo; Wang, Xuejun; Wang, Qiguang

2014-01-01

The fate and transfer of mercury in urban river is an important environmental concern. In this study, QWASI (Quantitative Water–Air–Sediment Interaction) model was selected to estimate the levels of total mercury and three mercury species in water and sediment, and was used to quantify the fluxes of mercury at water/air and sediment/water interfaces of an urban river. The predicted mercury levels in water and sediments were closed to the measured values. Water inflow, re-suspension of sediment and diffusion from sediment to water are major input sources of mercury in water. The net mercury transfer flux from water to air was 0.16 ng/(m 2 h). At the sediment/water interface, a net total mercury transfer of 1.32 ng/(m 2 h) from water to sediment was seen. In addition to the existing dynamic flux chambers measurement, this model method could provide a new perspective to identify the distribution and transfer of mercury in the urban river. -- Highlights: • QWASI could be a good tool to quantify transfer and fate of mercury in environment. • Distribution and flux of mercury species in an urban river was modeled. • Mercury in water mainly came from water inflow, sediment re-suspension and diffusion. • Net mercury transfer from water to air and sediment were 0.16 and 1.32 ng/(m 2 h). -- Quantitative Water–Air–Sediment Interaction model was used to quantify the transfer and fate of mercury in an urban river

Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

Energy Technology Data Exchange (ETDEWEB)

Hopperton, Kathryn E., E-mail: kathryn.hopperton@mail.utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Duncan, Robin E., E-mail: robin.duncan@uwaterloo.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Bazinet, Richard P., E-mail: richard.bazinet@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Archer, Michael C., E-mail: m.archer@utoronto.ca [Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada); Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 3E2 (Canada)

2014-01-15

Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from {sup 14}C-labeled acetate to those supplied exogenously as {sup 14}C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare

Fatty acid synthase plays a role in cancer metabolism beyond providing fatty acids for phospholipid synthesis or sustaining elevations in glycolytic activity

International Nuclear Information System (INIS)

Hopperton, Kathryn E.; Duncan, Robin E.; Bazinet, Richard P.; Archer, Michael C.

2014-01-01

Fatty acid synthase is over-expressed in many cancers and its activity is required for cancer cell survival, but the role of endogenously synthesized fatty acids in cancer is unknown. It has been suggested that endogenous fatty acid synthesis is either needed to support the growth of rapidly dividing cells, or to maintain elevated glycolysis (the Warburg effect) that is characteristic of cancer cells. Here, we investigate both hypotheses. First, we compared utilization of fatty acids synthesized endogenously from 14 C-labeled acetate to those supplied exogenously as 14 C-labeled palmitate in the culture medium in human breast cancer (MCF-7 and MDA-MB-231) and untransformed breast epithelial cells (MCF-10A). We found that cancer cells do not produce fatty acids that are different from those derived from exogenous palmitate, that these fatty acids are esterified to the same lipid and phospholipid classes in the same proportions, and that their distribution within neutral lipids is not different from untransformed cells. These results suggest that endogenously synthesized fatty acids do not fulfill a specific function in cancer cells. Furthermore, we observed that cancer cells excrete endogenously synthesized fatty acids, suggesting that they are produced in excess of requirements. We next investigated whether lipogenic activity is involved in the maintenance of high glycolytic activity by culturing both cancer and non-transformed cells under anoxic conditions. Although anoxia increased glycolysis 2–3 fold, we observed no concomitant increase in lipogenesis. Our results indicate that breast cancer cells do not have a specific qualitative or quantitative requirement for endogenously synthesized fatty acids and that increased de novo lipogenesis is not required to sustain elevations in glycolytic activity induced by anoxia in these cells. - Highlights: • Fatty acid synthase (FASN) is over-expressed in cancer but its function is unknown. • We compare utilization of

Tree Species with Photosynthetic Stems Have Greater Nighttime Sap Flux.

Science.gov (United States)

Chen, Xia; Gao, Jianguo; Zhao, Ping; McCarthy, Heather R; Zhu, Liwei; Ni, Guangyan; Ouyang, Lei

2018-01-01

An increasing body of evidence has shown that nighttime sap flux occurs in most plants, but the physiological implications and regulatory mechanism are poorly known. The significance of corticular photosynthesis has received much attention during the last decade, however, the knowledge of the relationship between corticular photosynthesis and nocturnal stem sap flow is limited at present. In this study, we divided seven tree species into two groups according to different photosynthetic capabilities: trees of species with ( Castanopsis hystrix, Michelia macclurei, Eucalyptus citriodora , and Eucalyptus grandis × urophylla ) and without ( Castanopsis fissa, Schima superba , and Acacia auriculiformis ) photosynthetic stems, and the sap flux ( J s ) and chlorophyll fluorescence parameters for these species were measured. One-way ANOVA analysis showed that the F v / F m (Maximum photochemical quantum yield of PSII) and Φ PSII (effective photochemical quantum yield of PSII) values were lower in non-photosynthetic stem species compared to photosynthetic stem species. The linear regression analysis showed that J s,d (daytime sap flux) and J s,n (nighttime sap flux) of non-photosynthetic stem species was 87.7 and 60.9% of the stem photosynthetic species. Furthermore, for a given daytime transpiration water loss, total nighttime sap flux was higher in species with photosynthetic stems (Slope SMA = 2.680) than in non-photosynthetic stems species (Slope SMA = 1.943). These results mean that stem corticular photosynthesis has a possible effect on the nighttime water flow, highlighting the important eco-physiological relationship between nighttime sap flux and corticular photosynthesis.

Flux agreement above a Scots pine plantation

Science.gov (United States)

Gay, L. W.; Vogt, R.; Bernhofer, Ch.; Blanford, J. H.

1996-03-01

The surface energy exchange of 12m high Scots pine plantation at Hartheim, Germany, was measured with a variety of methods during a 11-day period of fine weather in mid-May 1992. Net radiation and rate of thermal storage were measured with conventional net radiometers, soil heat flux discs and temperature-based storage models. The turbulent fluxes discussed in this report were obtained with an interchanging Bowen ratio energy budget system (BREB, at 14 m), two one-propeller eddy correlation systems (OPEC systems 1 and 2 at 17m), a 1-dimensional sonic eddy correlation system (SEC system 3) at 15 m, all on one “low” tower, and a 3-dimensional sonic eddy correlation system (SEC system 22) at 22 m on the “high” tower that was about 46 m distant. All systems measured sensible and latent heat (H and LE) directly, except for OPEC systems 1 and 2 which estimated LE as a residual term in the surface energy balance. Closure of turbulent fluxes from the two SEC systems was around 80% for daytime and 30% for night, with closure of 1-dimensional SEC system 3 exceeding that of 3-dimensional SEC system 22. The night measurements of turbulent fluxes contained considerable uncertainty, especially with the BREB system where measured gradients often yielded erroneous fluxes due to problems inherent in the method (i.e., computational instability as Bowen's ratio approaches -1). Also, both eddy correlation system designs (OPEC and SEC) appeared to underestimate |H| during stable conditions at night. In addition, both sonic systems (1- and 3-dimensional) underestimated |LE| during stable conditions. The underestimate of |H| at night generated residual estimates of OPEC LE containing a “phantom dew” error that erroneously decreased daily LE totals by about 10 percent. These special night problems are circumvented here by comparing results for daytime periods only, rather than for full days. To summarize, turbulent fluxes on the low tower from OPEC system 2 and the adjacent

Numerical simulations of river discharges, nutrient flux and nutrient dispersal in Jakarta Bay, Indonesia

International Nuclear Information System (INIS)

Wulp, Simon A. van der; Damar, Ario; Ladwig, Norbert; Hesse, Karl-J.

2016-01-01

The present application of numerical modelling techniques provides an overview of river discharges, nutrient flux and nutrient dispersal in Jakarta Bay. A hydrological model simulated river discharges with a total of 90 to 377 m 3 s −1 entering Jakarta Bay. Daily total nitrogen and total phosphorus loads ranged from 40 to 174 tons and 14 to 60 tons, respectively. Flow model results indicate that nutrient gradients are subject to turbulent mixing by tides and advective transport through circulation driven by wind, barotropic and baroclinic pressure gradients. The bulk of nutrient loads originate from the Citarum and Cisadane rivers flowing through predominantly rural areas. Despite lower nutrient loads, river discharges from the urban area of Jakarta exhibit the highest impact of nutrient concentrations in the near shore area of Jakarta Bay and show that nutrient concentrations were not only regulated by nutrient loads but were strongly regulated by initial river concentrations and local flow characteristics. - Highlights: • Full overview of river discharges, nutrient flux and nutrient levels in Jakarta Bay • Important overview of nutrient flux from individual rivers • Simulations identify the principal drivers of water circulation and nutrient gradient. • Nutrient dispersion model includes the local effects of the Java Sea current system.

Organic matter sources, fluxes and greenhouse gas exchange in the Oubangui River (Congo River basin)

Science.gov (United States)

Bouillon, S.; Yambélé, A.; Spencer, R. G. M.; Gillikin, D. P.; Hernes, P. J.; Six, J.; Merckx, R.; Borges, A. V.

2012-06-01

The Oubangui is a major tributary of the Congo River, draining an area of ~500 000 km2 mainly consisting of wooded savannahs. Here, we report results of a one year long, 2-weekly sampling campaign in Bangui (Central African Republic) since March 2010 for a suite of physico-chemical and biogeochemical characteristics, including total suspended matter (TSM), bulk concentration and stable isotope composition of particulate organic carbon (POC and δ13CPOC), particulate nitrogen (PN and δ15NPN), dissolved organic carbon (DOC and δ13CDOC), dissolved inorganic carbon (DIC and δ13CDIC), dissolved greenhouse gases (CO2, CH4 and N2O), and dissolved lignin composition. δ13C signatures of both POC and DOC showed strong seasonal variations (-30.6 to -25.8‰, and -31.8 to -27.1‰, respectively), but their different timing indicates that the origins of POC and DOC may vary strongly over the hydrograph and are largely uncoupled, differing up to 6‰ in δ13C signatures. Dissolved lignin characteristics (carbon-normalised yields, cinnamyl:vanillyl phenol ratios, and vanillic acid to vanillin ratios) showed marked differences between high and low discharge conditions, consistent with major seasonal variations in the sources of dissolved organic matter. We observed a strong seasonality in pCO2, ranging between 470 ± 203 ppm for Q production may be high enough to dominate the particulate organic carbon pool, and lower pCO2 values to near equilibrium values during low discharge conditions. The total annual flux of TSM, POC, PN, DOC and DIC are 2.33 Tg yr-1, 0.14 Tg C yr-1, 0.014 Tg N yr-1, 0.70 Tg C yr-1, and 0.49 Tg C yr-1, respectively. While our TSM and POC fluxes are similar to previous estimates for the Oubangui, DOC fluxes were ~30% higher and bicarbonate fluxes were ~35% lower than previous reports. DIC represented 58% of the total annual C flux, and under the assumptions that carbonate weathering represents 25% of the DIC flux and that CO2 from respiration drives

Energy Inputs Uncertainty: Total Amount, Distribution and Correlation Between Different Forms of Energy

Science.gov (United States)

Deng, Yue

2014-01-01

Describes solar energy inputs contributing to ionospheric and thermospheric weather processes, including total energy amounts, distributions and the correlation between particle precipitation and Poynting flux.

Comparison of nonflare solar soft x ray flux with 10.7-cm radio flux

International Nuclear Information System (INIS)

Donnelly, R.F.

1982-01-01

The similarities and differences of the nonflare solar 1- to 8-A X ray flux and the daily 10.7-cm Ottawa solar radio flux are examined. The radio flux is shown to be much less sensitive than the soft X ray flux on the average to the coronal emission of active regions located near or beyond the solar chromospheric limb relative to regions near the center of the solar disk. This is caused by the solar soft X ray emission's being optically thin while much of the 10.7-cm active region emission is from optical depths of tauapprox.1. The radio flux includes a large quiet sun flux which is emitted mostly from the tenuous chromosphere-corona transition region (Tapprox.10 4 --10 6 0 K) and partly from the cooler portions of the quiet corona Tapprox.1.5 x 10 6 0 K. Conversely, the solar soft X ray flux has a very small quiet sun component

An experimental study of high heat flux removal by shear-driven liquid films

Directory of Open Access Journals (Sweden)

Zaitsev Dmitry

2017-01-01

Full Text Available Intensively evaporating liquid films, moving under the friction of a co-current gas flow in a mini-channel (shear-driven liquid films, are promising for the use in cooling systems of modern semiconductor devices with high local heat release. In this work, the effect of various parameters, such as the liquid and gas flow rates and channel height, on the critical heat flux in the locally heated shear-driven water film has been studied. A record value of the critical heat flux of 1200 W/cm2 has been achieved in experiments. Heat leaks to the substrate and heat losses to the atmosphere in total do not exceed 25% for the heat flux above 400 W/cm2. Comparison of the critical heat fluxes for the shear-driven liquid film and for flow boiling in a minichannel shows that the critical heat flux is an order of magnitude higher for the shear-driven liquid film. This confirms the prospect of using shear-driven liquid films in the modern high-efficient cooling systems.

Effects of tropospheric aerosols on radiative flux calculations at UV and visible wavelengths

International Nuclear Information System (INIS)

Grossman, A.S.; Grant, K.E.

1994-08-01

The surface fluxes in the wavelength range 175 to 735nm have been calculated for an atmosphere which contains a uniformly mixed aerosol layer of thickness 1km at the earth's surface. Two different aerosol types were considered, a rural aerosol, and an urban aerosol. The visibility range for the aerosol layers was 95 to 15 km. Surface flux ratios (15km/95km) were in agreement with previously published results for the rural aerosol layer to within about 2%. The surface flux ratios vary from 7 to 14% for the rural aerosol layer and from 13 to 23% for the urban aerosol layer over the wavelength range. A tropospheric radiative forcing of about 1.3% of the total tropospheric flux was determined for the 95km to 15km visibility change in the rural aerosol layer, indicating the potential of tropospheric feedback effects on the surface flux changes. This effect was found to be negligible for the urban aerosol layer. Stratospheric layer heating rate changes due to visibility changes in either the rural or urban aerosol layer were found to be negligible

Response of actinides to flux changes in high-flux systems

International Nuclear Information System (INIS)

Sailor, W.C.

1993-01-01

When discussing the transmutation of actinides in accelerator-based transmutation of waste (ATW) systems, there has been some concern about the dynamics of the actinides under high transient fluxes. For a pure neptunium feed, it has been estimated that the 238 Np/ 237 Np ratio increase due to an increasing flux may lead to an unstable, positive reactivity growth. In this analysis, a perturbation method is used to calculate the response of the entire set of actinides in a general way that allows for more species than just neptunium. The time response of the system can be calculated; i.e., a plot of fuel composition and reactivity versus time after a change in flux can be made. The effects of fission products can also be included. The procedure is extremely accurate on short time scales (∼ 1000 s) for the flux levels we contemplate. Calculational results indicate that the reactivity insertions are always smaller than previously estimated

About Merging Threshold and Critical Flux Concepts into a Single One: The Boundary Flux

Directory of Open Access Journals (Sweden)

Marco Stoller

2014-01-01

Full Text Available In the last decades much effort was put in understanding fouling phenomena on membranes. One successful approach to describe fouling issues on membranes is the critical flux theory. The possibility to measure a maximum value of the permeate flux for a given system without incurring in fouling issues was a breakthrough in membrane process design. However, in many cases critical fluxes were found to be very low, lower than the economic feasibility of the process. The knowledge of the critical flux value must be therefore considered as a good starting point for process design. In the last years, a new concept was introduced, the threshold flux, which defines the maximum permeate flow rate characterized by a low constant fouling rate regime. This concept, more than the critical flux, is a new practical tool for membrane process designers. In this paper a brief review on critical and threshold flux will be reported and analyzed. And since the concepts share many common aspects, merged into a new concept, called the boundary flux, the validation will occur by the analysis of previously collected data by the authors, during the treatment of olive vegetation wastewater by ultrafiltration and nanofiltration membranes.

Study of the effect of the energy spectrum and of the total flux on the damage produced by neutrons in solids; Contribution a l'etude de l'influence du spectre et du flux integre sur les dommages crees par les neutrons dans les solides

Energy Technology Data Exchange (ETDEWEB)

Dulieu, P.C. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

1967-01-01

In the first part are studied the general relationships between the physical effects produced by neutrons in solids, and the total flux and neutron energy spectrum; some examples are given. The second part, describes the application to a silicon damage detector whose principle is to use the damage produced in a PIN Junction for measuring the neutron flux (intermediate and fast) received by the detector. Chapter I is devoted to the experimental determination of the energy given to the atoms by a primary in the silicon. The results and conclusions drawn from this determination make it possible to consider, in chapter II, the calculation of the detectors response characteristics.Chapter III deals with the measurement of the detectors response function and it is noted that good agreement is obtained between the calculation and experimental results. The whole of the second part constitutes a test of the methods presented in the first part. (author) [French] Dans la premiere partie, nous etudions les relations generales qui lient les effets physiques engendres par les neutrons dans les solides au flux integre et au spectre des neutrons et nous donnons des exemples d'utilisation. La deuxieme partie est une application au detecteur de dommages en silicium, dont le principe est d'utiliser les dommages crees dans une jonction PIN pour mesurer les flux de neutrons (intermediaires et rapides) recus par le detecteur. Le chapitre I est consacre a la determination experimentale de l'energie cedee aux atomes par un primaire dans le silicium. Les resultats et les conclusions que l'on peut tirer de cette determination permettent d'aborder, au chapitre II, le calcul de la fonction de reponse du detecteur. Le chapitre III porte sur la mesure de la fonction de reponse du detecteur et on constate qu'il y a un bon accord entre le calcul et l'experience. L'ensemble de la deuxieme partie constitue un test des methodes exposees dans la premiere partie

Extracting limits for the difuse non-electron neutrino flux from SNO data

Energy Technology Data Exchange (ETDEWEB)

Miguez, B.S.R.; Kemp, E.; Peres, O.L.G. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Fisica Gleb Wataghin

2009-07-01

Full text. There is a prediction of a diffuse neutrino flux yield from the time integration of all supernova already exploded in the past governed by stellar formation and supernovae occurrence rates. The spectral characteristics of these neutrinos differ from those from recent supernovae mainly in two features: the reduction in their fluxes and their energy 'redshift' due the expansion of the universe. Thus, despite the fact that one single supernova is a transient state, their cumulative effect produces a steady flux of diffuse neutrinos everywhere in universe. These neutrinos have never been observed before. Only upper limits on their fluxes have been reported by the collaborations operating neutrino telescopes. Recently the SNO experiment have made an analysis where the total flux of diffuse electron neutrinos has an upper limit of phi{sub e} <= 61-93 cm{sup -2} s{sup -1}, depending on a specific supernova model. At the present, the best limit for the diffuse flux of non-electron neutrinos is phi{sub x} <= 10{sub 4} cm{sup -2} s{sup -1}, resulted from an analysis of the Super-Kamiokande data. In this work we have extended the SNO analysis including the elastic scattering on electrons via neutral current interactions to extract information on diffuse flux of the non-electron neutrino flavours (i.e. muon and tauon neutrinos). We make a comparison among our results and others from different experiments (LVD, SK, LSD). (author)

Estimating the contribution of strong daily export events to total pollutant export from the United States in summer

Science.gov (United States)

Fang, Yuanyuan; Fiore, Arlene M.; Horowitz, Larry W.; Gnanadesikan, Anand; Levy, Hiram; Hu, Yongtao; Russell, Armistead G.

2009-12-01

While the export of pollutants from the United States exhibits notable variability from day to day and is often considered to be "episodic," the contribution of strong daily export events to total export has not been quantified. We use carbon monoxide (CO) as a tracer of anthropogenic pollutants in the Model of OZone And Related Tracers (MOZART) to estimate this contribution. We first identify the major export pathway from the United States to be through the northeast boundary (24-48°N along 67.5°W and 80-67.5°W along 48°N), and then analyze 15 summers of daily CO export fluxes through this boundary. These daily CO export fluxes have a nearly Gaussian distribution with a mean of 1100 Gg CO day-1 and a standard deviation of 490 Gg CO day-1. To focus on the synoptic variability, we define a "synoptic background" export flux equal to the 15 day moving average export flux and classify strong export days according to their fluxes relative to this background. As expected from Gaussian statistics, 16% of summer days are "strong export days," classified as those days when the CO export flux exceeds the synoptic background by one standard deviation or more. Strong export days contributes 25% to the total export, a value determined by the relative standard deviation of the CO flux distribution. Regressing the anomalies of the CO export flux through the northeast U.S. boundary relative to the synoptic background on the daily anomalies in the surface pressure field (also relative to a 15 day running mean) suggests that strong daily export fluxes are correlated with passages of midlatitude cyclones over the Gulf of Saint Lawrence. The associated cyclonic circulation and Warm Conveyor Belts (WCBs) that lift surface pollutants over the northeastern United States have been shown previously to be associated with long-range transport events. Comparison with observations from the 2004 INTEX-NA field campaign confirms that our model captures the observed enhancements in CO outflow

Heat flux microsensor measurements

Science.gov (United States)

Terrell, J. P.; Hager, J. M.; Onishi, S.; Diller, T. E.

1992-01-01

A thin-film heat flux sensor has been fabricated on a stainless steel substrate. The thermocouple elements of the heat flux sensor were nickel and nichrome, and the temperature resistance sensor was platinum. The completed heat flux microsensor was calibrated at the AEDC radiation facility. The gage output was linear with heat flux with no apparent temperature effect on sensitivity. The gage was used for heat flux measurements at the NASA Langley Vitiated Air Test Facility. Vitiated air was expanded to Mach 3.0 and hydrogen fuel was injected. Measurements were made on the wall of a diverging duct downstream of the injector during all stages of the hydrogen combustion tests. Because the wall and the gage were not actively cooled, the wall temperature reached over 1000 C (1900 F) during the most severe test.

Erosion of pyrolytic graphite and Ti-doped graphite due to high flux irradiation

International Nuclear Information System (INIS)

Ohtsuka, Yusuke; Ohashi, Junpei; Ueda, Yoshio; Isobe, Michiro; Nishikawa, Masahiro

1997-01-01

The erosion of pyrolytic graphite and titanium doped graphite RG-Ti above 1,780 K was investigated by 5 keV Ar beam irradiation with the flux from 4x10 19 to 1x10 21 m -2 ·s -1 . The total erosion yields were significantly reduced with the flux. This reduction would be attributed to the reduction of RES (radiation enhanced sublimation) yield, which was observed in the case of isotropic graphite with the flux dependence of RES yield of φ -0.26 (φ: flux) obtained in our previous work. The yield of pyrolytic graphite was roughly 30% higher than that of isotropic graphite below the flux of 10 20 m -2 ·s -1 whereas each yield approached to very close value at the highest flux of 1x10 21 m -2 ·s -1 . This result indicated that the effect of graphite structure on the RES yield, which was apparent in the low flux region, would disappear in the high flux region probably due to the disordering of crystal structure. In the case of irradiation to RG-Ti at 1,780 K, the surface undulations evolved with a mean height of about 3 μm at 1.2x10 20 m -2 ·s -1 , while at higher flux of 8.0x10 20 m -2 ·s -1 they were unrecognizable. These phenomena can be explained by the reduction of RES of graphite parts excluding TiC grains. (author)

Innovative CO2 Analyzer Technology for the Eddy Covariance Flux Monitor, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — We propose to build and evaluate NDIR Analyzers that can observe eddy covariance flux of CO2 from unmanned airborne platforms. For both phases, a total of four...

Flux of Cadmium through Euphausiids

International Nuclear Information System (INIS)

Benayoun, G.; Fowler, S.W.; Oregioni, B.

1976-01-01

Flux of the heavy metal cadmium through the euphausiid Meganyctiphanes norvegica was examined. Radiotracer experiments showed that cadmium can be accumulated either directly from water or through the food chain. When comparing equilibrium cadmium concentration factors based on stable element measurements with those obtained from radiotracer experiments, it is evident that exchange between cadmium in the water and that in euphausiid tissue is a relatively slow process, indicating that, in the long term, ingestion of cadmium will probably be the more important route for the accumulation of this metal. Approximately 10% of cadmium ingested by euphausiids was incorporated into internal tissues when the food source was radioactive Artemia. After 1 month cadmium, accumulated directly from water, was found to be most concentrated in the viscera with lesser amounts in eyes, exoskeleton and muscle, respectively. Use of a simple model, based on the assumption that cadmium taken in by the organism must equal cadmium released plus that accumulated in tissue, allowed assessment of the relative importance of various metabolic parameters in controlling the cadmium flux through euphausiids. Fecal pellets, due to their relatively high rate of production and high cadmium content, accounted for 84% of the total cadmium flux through M. norvegica. Comparisons of stable cadmium concentrations in natural euphausiid food and the organism's resultant fecal pellets indicate that the cadmium concentration in ingested material was increased nearly 5-fold during its passage through the euphausiid. From comparisons of all routes by which cadmium can be released from M. norvegica to the water column, it is concluded that fecal pellet deposition represents the principal mechanism effecting the downward vertical transport of cadmium by this species. (author)

Boosted Fast Flux Loop Alternative Cooling Assessment

Energy Technology Data Exchange (ETDEWEB)

Glen R. Longhurst; Donna Post Guillen; James R. Parry; Douglas L. Porter; Bruce W. Wallace

2007-08-01

The Gas Test Loop (GTL) Project was instituted to develop the means for conducting fast neutron irradiation tests in a domestic radiation facility. It made use of booster fuel to achieve the high neutron flux, a hafnium thermal neutron absorber to attain the high fast-to-thermal flux ratio, a mixed gas temperature control system for maintaining experiment temperatures, and a compressed gas cooling system to remove heat from the experiment capsules and the hafnium thermal neutron absorber. This GTL system was determined to provide a fast (E > 0.1 MeV) flux greater than 1.0E+15 n/cm2-s with a fast-to-thermal flux ratio in the vicinity of 40. However, the estimated system acquisition cost from earlier studies was deemed to be high. That cost was strongly influenced by the compressed gas cooling system for experiment heat removal. Designers were challenged to find a less expensive way to achieve the required cooling. This report documents the results of the investigation leading to an alternatively cooled configuration, referred to now as the Boosted Fast Flux Loop (BFFL). This configuration relies on a composite material comprised of hafnium aluminide (Al3Hf) in an aluminum matrix to transfer heat from the experiment to pressurized water cooling channels while at the same time providing absorption of thermal neutrons. Investigations into the performance this configuration might achieve showed that it should perform at least as well as its gas-cooled predecessor. Physics calculations indicated that the fast neutron flux averaged over the central 40 cm (16 inches) relative to ATR core mid-plane in irradiation spaces would be about 1.04E+15 n/cm2-s. The fast-to-thermal flux ratio would be in excess of 40. Further, the particular configuration of cooling channels was relatively unimportant compared with the total amount of water in the apparatus in determining performance. Thermal analyses conducted on a candidate configuration showed the design of the water coolant and

Assessing pesticide concentrations and fluxes in the stream of a small vineyard catchment - Effect of sampling frequency

International Nuclear Information System (INIS)

Rabiet, M.; Margoum, C.; Gouy, V.; Carluer, N.; Coquery, M.

2010-01-01

This study reports on the occurrence and behaviour of six pesticides and one metabolite in a small stream draining a vineyard catchment. Base flow and flood events were monitored in order to assess the variability of pesticide concentrations according to the season and to evaluate the role of sampling frequency on the evaluation of fluxes estimates. Results showed that dissolved pesticide concentrations displayed a strong temporal and spatial variability. A large mobilisation of pesticides was observed during floods, with total dissolved pesticide fluxes per event ranging from 5.7 x 10 -3 g/Ha to 0.34 g/Ha. These results highlight the major role of floods in the transport of pesticides in this small stream which contributed to more than 89% of the total load of diuron during August 2007. The evaluation of pesticide loads using different sampling strategies and method calculation, showed that grab sampling largely underestimated pesticide concentrations and fluxes transiting through the stream. - This work brings new insights about the fluxes of pesticides in surface water of a vineyard catchment, notably during flood events.

Assessing pesticide concentrations and fluxes in the stream of a small vineyard catchment - Effect of sampling frequency

Energy Technology Data Exchange (ETDEWEB)

Rabiet, M., E-mail: marion.rabiet@unilim.f [Cemagref, UR QELY, 3bis quai Chauveau, CP 220, F-69336 Lyon (France); Margoum, C.; Gouy, V.; Carluer, N.; Coquery, M. [Cemagref, UR QELY, 3bis quai Chauveau, CP 220, F-69336 Lyon (France)

2010-03-15

This study reports on the occurrence and behaviour of six pesticides and one metabolite in a small stream draining a vineyard catchment. Base flow and flood events were monitored in order to assess the variability of pesticide concentrations according to the season and to evaluate the role of sampling frequency on the evaluation of fluxes estimates. Results showed that dissolved pesticide concentrations displayed a strong temporal and spatial variability. A large mobilisation of pesticides was observed during floods, with total dissolved pesticide fluxes per event ranging from 5.7 x 10{sup -3} g/Ha to 0.34 g/Ha. These results highlight the major role of floods in the transport of pesticides in this small stream which contributed to more than 89% of the total load of diuron during August 2007. The evaluation of pesticide loads using different sampling strategies and method calculation, showed that grab sampling largely underestimated pesticide concentrations and fluxes transiting through the stream. - This work brings new insights about the fluxes of pesticides in surface water of a vineyard catchment, notably during flood events.

Atmospheric inversion of the surface CO2 flux with 13CO2 constraint

Science.gov (United States)

Chen, J. M.; Mo, G.; Deng, F.

2013-10-01

Observations of 13CO2 at 73 sites compiled in the GLOBALVIEW database are used for an additional constraint in a global atmospheric inversion of the surface CO2 flux using CO2 observations at 210 sites for the 2002-2004 period for 39 land regions and 11 ocean regions. This constraint is implemented using the 13CO2/CO2 flux ratio modeled with a terrestrial ecosystem model and an ocean model. These models simulate 13CO2 discrimination rates of terrestrial photosynthesis and respiration and ocean-atmosphere diffusion processes. In both models, the 13CO2 disequilibrium between fluxes to and from the atmosphere is considered due to the historical change in atmospheric 13CO2 concentration. For the 2002-2004 period, the 13CO2 constraint on the inversion increases the total land carbon sink from 3.40 to 3.70 Pg C yr-1 and decreases the total oceanic carbon sink from 1.48 to 1.12 Pg C yr-1. The largest changes occur in tropical areas: a considerable decrease in the carbon source in the Amazon forest, and this decrease is mostly compensated by increases in the ocean region immediately west of the Amazon and the southeast Asian land region. Our further investigation through different treatments of the 13CO2/CO2 flux ratio used in the inversion suggests that variable spatial distributions of the 13CO2 isotopic discrimination rate simulated by the models over land and ocean have considerable impacts on the spatial distribution of the inverted CO2 flux over land and the inversion results are not sensitive to errors in the estimated disequilibria over land and ocean.

Aeolian nutrient fluxes following wildfire in sagebrush steppe: implications for soil carbon storage

Directory of Open Access Journals (Sweden)

N. J. Hasselquist

2011-12-01

Full Text Available Pulses of aeolian transport following fire can profoundly affect the biogeochemical cycling of nutrients in semi-arid and arid ecosystems. Our objective was to determine horizontal nutrient fluxes occurring in the saltation zone during an episodic pulse of aeolian transport that occurred following a wildfire in a semi-arid sagebrush steppe ecosystem in southern Idaho, USA. We also examined how temporal trends in nutrient fluxes were affected by changes in particle sizes of eroded mass as well as nutrient concentrations associated with different particle size classes. In the burned area, total carbon (C and nitrogen (N fluxes were as high as 235 g C m⁻¹ d⁻¹ and 19 g N m⁻¹ d⁻¹ during the first few months following fire, whereas C and N fluxes were negligible in an adjacent unburned area throughout the study. Temporal variation in C and N fluxes following fire was largely attributable to the redistribution of saltation-sized particles. Total N and organic C concentrations in the soil surface were significantly lower in the burned relative to the unburned area one year after fire. Our results show how an episodic pulse of aeolian transport following fire can affect the spatial distribution of soil C and N, which, in turn, can have important implications for soil C storage. These findings demonstrate how an ecological disturbance can exacerbate a geomorphic process and highlight the need for further research to better understand the role aeolian transport plays in the biogeochemical cycling of C and N in recently burned landscapes.

Evolution of the magnetic helicity flux during the formation and eruption of flux ropes

Energy Technology Data Exchange (ETDEWEB)

Romano, P. [INAF—Osservatorio Astrofisico di Catania, Via S. Sofia 78, I-95123 Catania (Italy); Zuccarello, F. P. [Centre for Mathematical Plasma-Astrophysics, KU Leuven, Celestijnenlaan 200B, B-3001 Leuven (Belgium); Guglielmino, S. L.; Zuccarello, F., E-mail: paolo.romano@oact.inaf.it [Dipartimento di Fisica e Astronomia—Sezione Astrofisica, Università di Catania, Via S. Sofia 78, I-95123 Catania (Italy)

2014-10-20

We describe the evolution and the magnetic helicity flux for two active regions (ARs) since their appearance on the solar disk: NOAA 11318 and NOAA 11675. Both ARs hosted the formation and destabilization of magnetic flux ropes. In the former AR, the formation of the flux rope culminated in a flare of C2.3 GOES class and a coronal mass ejection (CME) observed by Large Angle and Spectrometric Coronagraph Experiment. In the latter AR, the region hosting the flux rope was involved in several flares, but only a partial eruption with signatures of a minor plasma outflow was observed. We found a different behavior in the accumulation of the magnetic helicity flux in the corona, depending on the magnetic configuration and on the location of the flux ropes in the ARs. Our results suggest that the complexity and strength of the photospheric magnetic field is only a partial indicator of the real likelihood of an AR producing the eruption of a flux rope and a subsequent CME.

Leaf, branch, stand and landscape scale measurements of volatile organic compound fluxes from U. S. woodlands

Energy Technology Data Exchange (ETDEWEB)

Guenther, A.; Greenberg, J.; Harley, P.; Helmig, D.; Klinger, L.; Vierling, L.; Zimmerman, P. [National Center for Atmospheric Research, Boulder, CO (United States). Atmospheric Chemistry Div.; Geron, C. [Environmental Protection Agency, Research Triangle Park, NC (United States)

1996-01-01

Natural volatile organic compound (VOC) fluxes were measured in three U.S. woodlands. Fluxes from individual leaves and branches were estimated with enclosure techniques and used to initialize and evaluate VOC emission model estimates. Ambient measurements were used to estimate above canopy fluxes for entire stands. A total of 78 VOCs were identified, with hexenol derivatives being the most commonly observed oxygenated compounds. There was also evidence of high rates of isoprene emission and high rates of monoterpenes in some genera of trees. Model predictions of diurnal variations were within + or - 35 per cent of observed flux variations. Fluxes predicted by a recent version of a biogenic emission model were within 10 per cent to 50 per cent of observed fluxes, leading to the conclusion that existing databases can provide isoprene and monoterpene emission rate potentials within acceptable limits for the dominant plant species at these three woodland sites. 21 refs., 5 tabs., 2 figs.

EVOLUTION OF SPINNING AND BRAIDING HELICITY FLUXES IN SOLAR ACTIVE REGION NOAA 10930

Energy Technology Data Exchange (ETDEWEB)

Ravindra, B. [Indian Institute of Astrophysics, Koramangala, Bangalore 560 034 (India); Yoshimura, Keiji [Department of Physics, Montana State University Bozeman, MT 59717 (United States); Dasso, Sergio, E-mail: ravindra@iiap.res.in, E-mail: yosimura@solar.physics.montana.edu, E-mail: dasso@df.uba.ar [Instituto de Astronomia y Fisica del Espacio (CONICET-UBA), 1428 Buenos Aires (Argentina)

2011-12-10

The line-of-sight magnetograms from Solar Optical Telescope Narrowband Filter Imager observations of NOAA Active Region 10930 have been used to study the evolution of spinning and braiding helicities over a period of five days starting from 2006 December 9. The north (N) polarity sunspot was the follower and the south (S) polarity sunspot was the leader. The N-polarity sunspot in the active region was rotating in the counterclockwise direction. The rate of rotation was small during the first two days of observations and it increased up to 8 Degree-Sign hr{sup -1} on the third day of the observations. On the fourth and fifth days it remained at 4 Degree-Sign hr{sup -1} with small undulations in its magnitude. The sunspot rotated about 260 Degree-Sign in the last three days. The S-polarity sunspot did not complete more than 20 Degree-Sign in five days. However, it changed its direction of rotation five times over a period of five days and injected both the positive and negative type of spin helicity fluxes into the corona. Through the five days, both the positive and negative sunspot regions injected equal amounts of spin helicity. The total injected helicity is predominantly negative in sign. However, the sign of the spin and braiding helicity fluxes computed over all the regions were reversed from negative to positive five times during the five-day period of observations. The reversal in spinning helicity flux was found before the onset of the X3.4-class flare, too. Though, the rotating sunspot has been observed in this active region, the braiding helicity has contributed more to the total accumulated helicity than the spinning helicity. The accumulated helicity is in excess of -7 Multiplication-Sign 10{sup 43} Mx{sup 2} over a period of five days. Before the X3.4-class flare that occurred on 2006 December 13, the rotation speed and spin helicity flux increased in the S-polarity sunspot. Before the flare, the total injected helicity was larger than -6

Characteristics of heat flux and particle flux to the divertor in H-mode of JT-60U

International Nuclear Information System (INIS)

Itami, K.; Hosogane, N.; Asakura, N.; Kubo, H.; Tsuji, S.; Shimada, M.

1995-01-01

Heat flux and particle flux behavior in H-mode is studied in a comparative manner. It was confirmed that the multiple peak structure of heat flux during ELM activity has a role in reducing the average value of a peak heat flux at the divertor. In order to characterize heat and particle flux during ELM activity, the ELM part and the steady state part of heat flux and particle flux were determined and statistically analyzed. A large in-out asymmetry of peak ELM heat flux density was found. The asymmetry is almost unaffected by the ion grad-B drift direction. In-out asymmetry of both ELM and steady-state parts of the particle flux were found to be similar. ((orig.))

Magnitude and directional measures of water and Cr(VI) fluxes by passive flux meter.

Science.gov (United States)

Campbell, Timothy J; Hatfield, Kirk; Klammler, Harald; Annable, Michael D; Rao, P S C

2006-10-15

A new configuration of the passive fluxmeter (PFM) is presented that provides for simultaneous measurements of both the magnitude and the direction of ambient groundwater specific discharge qo and Cr(VI) mass flux J(Cr). The PFM is configured as a cylindrical unit with an interior divided into a center section and three outer sectors, each packed with a granular anion exchange resin having high sorption capacity for the Cr(VI) oxyanions CrO4(2-) and HCrO4-. The sorbent in the center section is preloaded with benzoate as the "resident" tracer. Laboratory experiments were conducted in which PFMs were placed in porous packed bed columns, through which was passed a measured volume of synthetic groundwater containing Cr(VI). During the deployment period, some of the resident tracer is depleted while the Cr(VI) is sorbed. The resin was then removed from the four sectors separately and extracted to determine the "captured" mass of Cr(VI) and the residual mass of the resident tracer in each. Cumulative specific discharge, q0t, values were assessed using the residual mass of benzoate retained in the center section. The direction of this discharge theta was ascertained from the mass distribution of benzoate intercepted and retained in the outer three sections of the PFM. Cumulative chromium fluxes, J(Cr)t, were quantified using the total Cr(VI) mass intercepted and retained on the PFM. Experiments produced an average measurement error for direction theta of 3 degrees +/- 14 degrees, while the average measurement errors for q0 and J(Cr) were, respectively, -8% +/- 15% and -12% +/- 23%. Results demonstrate the potential utility of the new PFM configuration for characterizing groundwater and contaminant fluxes.

Ecosystem carbon and radiative fluxes: a global synthesis based on the FLUXNET network.

Science.gov (United States)

Cescatti, A.

2009-04-01

Solar radiation is the most important environmental factor driving the temporal and spatial variability of the gross primary productivity (GPP) in terrestrial ecosystems. At the ecosystem scale, the light use efficiency (LUE) depends not only on radiation quantity but also on radiation "quality" both in terms of spectral composition and angular distribution. The day-to-day variations in LUE are largely determined by changes in the ratio of diffuse to total radiation. The relative importance of the concurrent variation in total incoming radiation and in LUE is essential to estimate the sign and the magnitude of the GPP sensitivity to radiation. Despite the scientific relevance of this issue, a global assessment on the sensitivity of GPP to the variations of Phar is still missing. Such an analysis is needed to improve our understanding of the current and future impacts of aerosols and cloud cover on the spatio-temporal variability of GPP. The current availability of ecosystem carbon fluxes, together with separate measurements of incoming direct and diffuse Phar at a large number of flux sites, offers the unique opportunity to extend the previous investigation, both in terms of ecosystem, spatial and climate coverage, and to address questions about the internal (e.g. leaf area index, canopy structure) and external (e.g. cloudiness, covarying meteorology) factors affecting the ecosystem sensitivity to radiation geometry. For this purpose half-hourly measurements of carbon fluxes and radiation have been analyzed at about 220 flux sites for a total of about 660 site-years. This analysis demonstrates that the sensitivity of GPP to incoming radiation varies across the different plant functional types and is correlated with the leaf area index and the local climatology. In particular, the sensitivity of GPP to changes in incoming diffuse light maximizes for the broadleaved forests of the Northern Hemisphere.

Surface Flux Measurements at King Sejong Station in West Antarctica

Science.gov (United States)

Choi, T.; Lee, B.; Lee, H.; Shim, J.

2004-12-01

The Antarctic Peninsula is important in terms of global warming research due to pronounced increase of air temperature over the last century. The first eddy covariance system was established and turbulent fluxes of heat, water vapor, CO2 and momentum have been measured at King Sejong Station (62 \\deg 13øØS, 58 \\deg 47øØW) located in the northern edge of the Antarctic Peninsula since December in 2002. Our objectives are to better understand the interactions between the Antarctic land surface and the atmosphere and to test the feasibility of the long-term operation of eddy covariance system under extreme weather conditions. Various lichens cover the study area and the dominant species is Usnea fasciata-Himantormia. Based on the analyses on turbulent statistics such as integral turbulence characteristics of vertical velocity (w) and heat (T), stationarity test and investigation of correlation coefficient, they follow the Monin-Obukhov similarity and eddy covariance flux data were reliable. About 50 % of total retrieved sensible heat flux data could be used for further analysis. We will report on seasonal variations of energy and mass fluxes and environmental variables. In addition, factors controlling these fluxes will be presented. Acknowledgement: This study was supported by ¡rEnvironmental Monitoring on Human Impacts at the King Sejong Station, Antarctica¡_ (Project PP04102 of Korea Polar Research Institute) and ¡rEco-technopia 21 project¡_ (Ministry of Environment of Korea).

Use of Principal Components Analysis to Explain Controls on Nutrient Fluxes to the Chesapeake Bay

Science.gov (United States)

Rice, K. C.; Mills, A. L.

2017-12-01

The Chesapeake Bay watershed, on the east coast of the United States, encompasses about 166,000-square kilometers (km2) of diverse land use, which includes a mixture of forested, agricultural, and developed land. The watershed is now managed under a Total Daily Maximum Load (TMDL), which requires implementation of management actions by 2025 that are sufficient to reduce nitrogen, phosphorus, and suspended-sediment fluxes to the Chesapeake Bay and restore the bay's water quality. We analyzed nutrient and sediment data along with land-use and climatic variables in nine sub watersheds to better understand the drivers of flux within the watershed and to provide relevant management implications. The nine sub watersheds range in area from 300 to 30,000 km2, and the analysis period was 1985-2014. The 31 variables specific to each sub watershed were highly statistically significantly correlated, so Principal Components Analysis was used to reduce the dimensionality of the dataset. The analysis revealed that about 80% of the variability in the whole dataset can be explained by discharge, flux, and concentration of nutrients and sediment. The first two principal components (PCs) explained about 68% of the total variance. PC1 loaded strongly on discharge and flux, and PC2 loaded on concentration. The PC scores of both PC1 and PC2 varied by season. Subsequent analysis of PC1 scores versus PC2 scores, broken out by sub watershed, revealed management implications. Some of the largest sub watersheds are largely driven by discharge, and consequently large fluxes. In contrast, some of the smaller sub watersheds are more variable in nutrient concentrations than discharge and flux. Our results suggest that, given no change in discharge, a reduction in nutrient flux to the streams in the smaller watersheds could result in a proportionately larger decrease in fluxes of nutrients down the river to the bay, than in the larger watersheds.

Linking lowermost mantle structure, core-mantle boundary heat flux and mantle plume formation

Science.gov (United States)

Li, Mingming; Zhong, Shijie; Olson, Peter

2018-04-01

The dynamics of Earth's lowermost mantle exert significant control on the formation of mantle plumes and the core-mantle boundary (CMB) heat flux. However, it is not clear if and how the variation of CMB heat flux and mantle plume activity are related. Here, we perform geodynamic model experiments that show how temporal variations in CMB heat flux and pulses of mantle plumes are related to morphologic changes of the thermochemical piles of large-scale compositional heterogeneities in Earth's lowermost mantle, represented by the large low shear velocity provinces (LLSVPs). We find good correlation between the morphologic changes of the thermochemical piles and the time variation of CMB heat flux. The morphology of the thermochemical piles is significantly altered during the initiation and ascent of strong mantle plumes, and the changes in pile morphology cause variations in the local and the total CMB heat flux. Our modeling results indicate that plume-induced episodic variations of CMB heat flux link geomagnetic superchrons to pulses of surface volcanism, although the relative timing of these two phenomena remains problematic. We also find that the density distribution in thermochemical piles is heterogeneous, and that the piles are denser on average than the surrounding mantle when both thermal and chemical effects are included.

A technical basis for the flux corrected local conditions critical heat flux correlation

International Nuclear Information System (INIS)

Luxat, J.C.

2008-01-01

The so-called 'flux-corrected' local conditions CHF correlation was developed at Ontario Hydro in the 1980's and was demonstrated to successfully correlate the Onset of Intermittent Dryout (OID) CHF data for 37-element fuel with a downstream-skewed axial heat flux distribution. However, because the heat flux correction factor appeared to be an ad-hoc, albeit a successful modifying factor in the correlation, there was reluctance to accept the correlation more generally. This paper presents a thermalhydraulic basis, derived from two-phase flow considerations, that supports the appropriateness of the heat flux correction as a local effects modifying factor. (author)

Compact neutron flux monitor

International Nuclear Information System (INIS)

Madhavi, V.; Phatak, P.R.; Bahadur, C.; Bayala, A.K.; Jakati, R.K.; Sathian, V.

2003-01-01

Full text: A compact size neutron flux monitor has been developed incorporating standard boards developed for smart radiation monitors. The sensitivity of the monitors is 0.4cps/nV. It has been tested up to 2075 nV flux with standard neutron sources. It shows convincing results even in high flux areas like 6m away from the accelerator in RMC (Parel) for 106/107 nV. These monitors have a focal and remote display, alarm function with potential free contacts for centralized control and additional provision of connectivity via RS485/Ethernet. This paper describes the construction, working and results of the above flux monitor

In-core neutron flux measurements at PARR using self powered neutron detector

International Nuclear Information System (INIS)

Hussain, A.; Ansari, S.A.

1989-10-01

This report describes experimental reactor physics measure ments at PARR using the in-core neutron detectors. Rhodium self powered neutron detectors (SPND) were used in the PARR core and several measurements were made aimed at detector calibration, response time determination and neutron flux measurements. The detectors were calibrated at low power using gold foils and full power by the thermal channel. Based on this calibration it was observed that the detector response remains almost linear throughout the power range. The self powered detectors were used for on-line determination of absolute neutron flux in the core as well as the spatial distribution of neutron flux or reactor power. The experimental, axial and horizontal flux mapping results at certain locations in the core are presented. The total response time of rhodium detector was experimentally determined to be about 5 minutes, which agree well with the theoretical results. Because of longer response time of SPND of the detectors it is not possible to use them in the reactor protection system. (author). 10 figs

Sensitivity of upper atmospheric emissions calculations to solar/stellar UV flux

Directory of Open Access Journals (Sweden)

Barthelemy Mathieu

2014-01-01

Full Text Available The solar UV (UltraViolet flux, especially the EUV (Extreme UltraViolet and FUV (Far UltraViolet components, is one of the main energetic inputs for planetary upper atmospheres. It drives various processes such as ionization, or dissociation which give rise to upper atmospheric emissions, especially in the UV and visible. These emissions are one of the main ways to investigate the upper atmospheres of planets. However, the uncertainties in the flux measurement or modeling can lead to biased estimates of fundamental atmospheric parameters, such as concentrations or temperatures in the atmospheres. We explore the various problems that can be identified regarding the uncertainties in solar/stellar UV flux by considering three examples. The worst case appears when the solar reflection component is dominant in the recorded spectrum as is seen for outer solar system measurements from HST (Hubble Space Telescope. We also show that the estimation of some particular line parameters (intensity and shape, especially Lyman α, is crucial, and that both total intensity and line profile are useful. In the case of exoplanets, the problem is quite critical since the UV flux of their parent stars is often very poorly known.

Flux compactifications and generalized geometries

International Nuclear Information System (INIS)

Grana, Mariana

2006-01-01

Following the lectures given at CERN Winter School 2006, we present a pedagogical overview of flux compactifications and generalized geometries, concentrating on closed string fluxes in type II theories. We start by reviewing the supersymmetric flux configurations with maximally symmetric four-dimensional spaces. We then discuss the no-go theorems (and their evasion) for compactifications with fluxes. We analyse the resulting four-dimensional effective theories for Calabi-Yau and Calabi-Yau orientifold compactifications, concentrating on the flux-induced superpotentials. We discuss the generic mechanism of moduli stabilization and illustrate with two examples: the conifold in IIB and a T 6 /(Z 3 x Z 3 ) torus in IIA. We finish by studying the effective action and flux vacua for generalized geometries in the context of generalized complex geometry

Flux compactifications and generalized geometries

Energy Technology Data Exchange (ETDEWEB)

Grana, Mariana [Service de Physique Theorique, CEA/Saclay, 91191 Gif-sur-Yvette Cedex (France)

2006-11-07

Following the lectures given at CERN Winter School 2006, we present a pedagogical overview of flux compactifications and generalized geometries, concentrating on closed string fluxes in type II theories. We start by reviewing the supersymmetric flux configurations with maximally symmetric four-dimensional spaces. We then discuss the no-go theorems (and their evasion) for compactifications with fluxes. We analyse the resulting four-dimensional effective theories for Calabi-Yau and Calabi-Yau orientifold compactifications, concentrating on the flux-induced superpotentials. We discuss the generic mechanism of moduli stabilization and illustrate with two examples: the conifold in IIB and a T{sup 6} /(Z{sub 3} x Z{sub 3}) torus in IIA. We finish by studying the effective action and flux vacua for generalized geometries in the context of generalized complex geometry.

Design of a Modular E-Core Flux Concentrating Axial Flux Machine: Preprint

Energy Technology Data Exchange (ETDEWEB)

Husain, Tausif; Sozer, Yilmaz; Husain, Iqbal; Muljadi, Eduard

2015-08-24

In this paper a novel E-Core axial flux machine is proposed. The machine has a double-stator, single-rotor configuration with flux-concentrating ferrite magnets and pole windings across each leg of an E-Core stator. E-Core stators with the proposed flux-concentrating rotor arrangement result in better magnet utilization and higher torque density. The machine also has a modular structure facilitating simpler construction. This paper presents a single-phase and a three-phase version of the E-Core machine. Case studies for a 1.1-kW, 400-rpm machine for both the single-phase and three-phase axial flux machines are presented. The results are verified through 3D finite element analysis. facilitating simpler construction. This paper presents a single-phase and a three-phase version of the E-Core machine. Case studies for a 1.1-kW, 400-rpm machine for both the single-phase and three-phase axial flux machines are presented. The results are verified through 3D finite element analysis.

A constant heat flux plasma limiter for TEXTOR

International Nuclear Information System (INIS)

Mioduszewski, P.

1980-10-01

In future large tokamak machines heat removal from the plasma is going to play an important role. In TEXTOR the total plasma power is expected to be in the range of 0.5-2.5 MW. Typical fractions of about 50% of this power have to be removed from the plasma by limiters. The power flux from the limiter scrape-off layer to the limiter surface decays rapidly with distance into the scrape-off layer resulting in a highly space-dependent heat load on the limiter. Therefore, limiters are shaped in a way to smooth of the heat load, and the ideal limiter shape should produce a constant heat flux over the whole limiter surface. The ideally shaped limiter offers a better chance to handle the high heat loads with the preferred materials like stainless steel (or inconel 625 as in the case of TEXTOR). (orig./GG)

Asymmetry in the convective energy fluxes due to electrostatic and magnetic fluctuations in magnetized plasmas

International Nuclear Information System (INIS)

Smolyakov, A.I.; Hirose, A.

1993-01-01

The structure of the energy balance equation for a magnetically confined plasma in the presence of electromagnetic fluctuations is investigated by using the drift kinetic equation. The convective energy fluxes, one caused by E x B electrostatic turbulence and the other by shear-Alfven type magnetic turbulence, are asymmetric: For low frequency electrostatic turbulence, the convective energy flux has a unique numerical factor 3/2, while the convective energy flux induced by magnetic turbulence has a numerical factor 5/2. As expected, in the drift approximation, turbulent heating by the longitudinal electric field is the only anomalous source term in the total energy balance equation. (Author)

Mapping carbon flux uncertainty and selecting optimal locations for future flux towers in the Great Plains

Science.gov (United States)

Gu, Yingxin; Howard, Daniel M.; Wylie, Bruce K.; Zhang, Li

2012-01-01

Flux tower networks (e. g., AmeriFlux, Agriflux) provide continuous observations of ecosystem exchanges of carbon (e. g., net ecosystem exchange), water vapor (e. g., evapotranspiration), and energy between terrestrial ecosystems and the atmosphere. The long-term time series of flux tower data are essential for studying and understanding terrestrial carbon cycles, ecosystem services, and climate changes. Currently, there are 13 flux towers located within the Great Plains (GP). The towers are sparsely distributed and do not adequately represent the varieties of vegetation cover types, climate conditions, and geophysical and biophysical conditions in the GP. This study assessed how well the available flux towers represent the environmental conditions or "ecological envelopes" across the GP and identified optimal locations for future flux towers in the GP. Regression-based remote sensing and weather-driven net ecosystem production (NEP) models derived from different extrapolation ranges (10 and 50%) were used to identify areas where ecological conditions were poorly represented by the flux tower sites and years previously used for mapping grassland fluxes. The optimal lands suitable for future flux towers within the GP were mapped. Results from this study provide information to optimize the usefulness of future flux towers in the GP and serve as a proxy for the uncertainty of the NEP map.

Human Effects and Soil Surface CO2 fluxes in Tropical Urban Green Areas, Singapore

Science.gov (United States)

Ng, Bernard; Gandois, Laure; Kai, Fuu Ming; Chua, Amy; Cobb, Alex; Harvey, Charles; Hutyra, Lucy

2013-04-01

Urban green spaces are appreciated for their amenity value, with increasing interest in the ecosystem services they could provide (e.g. climate amelioration and increasingly as possible sites for carbon sequestration). In Singapore, turfgrass occupies approximately 20% of the total land area and is readily found on both planned and residual spaces. This project aims at understanding carbon fluxes in tropical urban green areas, including controls of soil environmental factors and the effect of urban management techniques. Given the large pool of potentially labile carbon, management regimes are recognised to have an influence on soil environmental factors (temperature and moisture), this would affect soil respiration and feedbacks to the greenhouse effect. A modified closed dynamic chamber method was employed to measure total soil respiration fluxes. In addition to soil respiration rates, environmental factors such as soil moisture and temperature, and ambient air temperature were monitored for the site in an attempt to evaluate their control on the observed fluxes. Measurements of soil-atmosphere CO2 exchanges are reported for four experimental plots within the Singtel-Kranji Radio Transmission Station (103o43'49E, 1o25'53N), an area dominated by Axonopus compressus. Different treatments such as the removal of turf, and application of clippings were effected as a means to determine the fluxes from the various components (respiration of soil and turf, and decomposition of clippings), and to explore the effects of human intervention on observed effluxes. The soil surface CO2 fluxes observed during the daylight hours ranges from 2.835 + 0.772 umol m-2 s-1 for the bare plot as compared to 6.654 + 1.134 umol m-2 s-1 for the turfed plot; this could be attributed to both autotrophic and heterotrophic respiration. Strong controls of both soil temperature and soil moisture are observed on measured soil fluxes. On the base soils, fluxes were positively correlated to soil

EL-2 reactor: Thermal neutron flux distribution; EL-2: Repartition du flux de neutrons thermiques

Energy Technology Data Exchange (ETDEWEB)

Rousseau, A; Genthon, J P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1958-07-01

The flux distribution of thermal neutrons in EL-2 reactor is studied. The reactor core and lattices are described as well as the experimental reactor facilities, in particular, the experimental channels and special facilities. The measurement shows that the thermal neutron flux increases in the central channel when enriched uranium is used in place of natural uranium. However the thermal neutron flux is not perturbed in the other reactor channels by the fuel modification. The macroscopic flux distribution is measured according the radial positioning of fuel rods. The longitudinal neutron flux distribution in a fuel rod is also measured and shows no difference between enriched and natural uranium fuel rods. In addition, measurements of the flux distribution have been effectuated for rods containing other material as steel or aluminium. The neutron flux distribution is also studied in all the experimental channels as well as in the thermal column. The determination of the distribution of the thermal neutron flux in all experimental facilities, the thermal column and the fuel channels has been made with a heavy water level of 1825 mm and is given for an operating power of 1000 kW. (M.P.)

Particle acceleration in relativistic magnetic flux-merging events

Science.gov (United States)

Lyutikov, Maxim; Sironi, Lorenzo; Komissarov, Serguei S.; Porth, Oliver

2017-12-01

Using analytical and numerical methods (fluid and particle-in-cell simulations) we study a number of model problems involving merger of magnetic flux tubes in relativistic magnetically dominated plasma. Mergers of current-carrying flux tubes (exemplified by the two-dimensional `ABC' structures) and zero-total-current magnetic flux tubes are considered. In all cases regimes of spontaneous and driven evolution are investigated. We identify two stages of particle acceleration during flux mergers: (i) fast explosive prompt X-point collapse and (ii) ensuing island merger. The fastest acceleration occurs during the initial catastrophic X-point collapse, with the reconnection electric field of the order of the magnetic field. During the X-point collapse, particles are accelerated by charge-starved electric fields, which can reach (and even exceed) values of the local magnetic field. The explosive stage of reconnection produces non-thermal power-law tails with slopes that depend on the average magnetization . For plasma magnetization 2$ the spectrum power-law index is 2$ ; in this case the maximal energy depends linearly on the size of the reconnecting islands. For higher magnetization, 2$ , the spectra are hard, , yet the maximal energy \\text{max}$ can still exceed the average magnetic energy per particle, , by orders of magnitude (if is not too close to unity). The X-point collapse stage is followed by magnetic island merger that dissipates a large fraction of the initial magnetic energy in a regime of forced magnetic reconnection, further accelerating the particles, but proceeds at a slower reconnection rate.

Quality control of total alpha and beta activity analysis in water using the Proportional Counter of Gaseous Flux technique; Controle de qualidade das análises de atividades alfa e beta total em água pela técnica do Contador Proporcional de Fluxo Gasoso

Energy Technology Data Exchange (ETDEWEB)

Neto, Alberto P.; Bastos, Thiago M.R.; Santos, Thiago O.; Filho, Crescêncio A.S.; França, Elvis J. de, E-mail: albertopereiran@gmail.com, E-mail: thiagomrbastos@gmail.com, E-mail: thiago_cbufpe@yahoo.com.br, E-mail: candrade@cnen.gov.br, E-mail: ejfranca@cnen.gov.br [Centro Regional de Ciências Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

2017-07-01

The analytical capacity of the CRCN (Centro Regional de Ciências Nucleares do Nordeste) for quantification of total alpha and beta concentrations in water samples using the Proportional Counter of Gaseous Flux is presented. To demonstrate the traceability of the results, the National Intercomparison Program (PNI) samples were used, promoted by the Institute of Radioprotection and Dosimetry (IRD) from which the Regional Nuclear Science Center of the Northeast (CRCN-NE) participates since 2011. The results reported to the PNI demonstrate the analytical capacity of the CRCN to detect the emission of alpha and beta radioactivities in water within the potability standards in analytical detection of quantification in radioactivity by the technique of the Proportional Flow Counter.

Flux Pinning in Superconductors

CERN Document Server

Matsushita, Teruo

2007-01-01

The book covers the flux pinning mechanisms and properties and the electromagnetic phenomena caused by the flux pinning common for metallic, high-Tc and MgB2 superconductors. The condensation energy interaction known for normal precipitates or grain boundaries and the kinetic energy interaction proposed for artificial Nb pins in Nb-Ti, etc., are introduced for the pinning mechanism. Summation theories to derive the critical current density are discussed in detail. Irreversible magnetization and AC loss caused by the flux pinning are also discussed. The loss originally stems from the ohmic dissipation of normal electrons in the normal core driven by the electric field induced by the flux motion. The readers will learn why the resultant loss is of hysteresis type in spite of such mechanism. The influence of the flux pinning on the vortex phase diagram in high Tc superconductors is discussed, and the dependencies of the irreversibility field are also described on other quantities such as anisotropy of supercondu...

[Effects of biological soil crust at different succession stages in hilly region of Loess Plateau on soil CO2 flux].

Science.gov (United States)

Wang, Ai-Guo; Zhao, Yun-Ge; Xu, Ming-Xiang; Yang, Li-Na; Ming, Jiao

2013-03-01

Biological soil crust (biocrust) is a compact complex layer of soil, which has photosynthetic activity and is one of the factors affecting the CO2flux of soil-atmosphere interface. In this paper, the soil CO, flux under the effects of biocrust at different succession stages on the re-vegetated grassland in the hilly region of Loess Plateau was measured by a modified LI-8100 automated CO, flux system. Under light condition, the soil CO2 flux under effects of cyanobacteria crust and moss crust was significantly decreased by 92% and 305%, respectively, as compared with the flux without the effects of the biocrusts. The decrement of the soil CO, flux by the biocrusts was related to the biocrusts components and their biomass. Under the effects of dark colored cyanobacteria crust and moss crust, the soil CO2 flux was decreased by 141% and 484%, respectively, as compared with that in bare land. The diurnal curve of soil CO2 flux under effects of biocrusts presented a trend of 'drop-rise-drop' , with the maximum carbon uptake under effects of cyanobacteria crust and moss crust being 0.13 and -1.02 micromol CO2.m-2.s-1 and occurred at about 8:00 and 9:00 am, respectively, while that in bare land was unimodal. In a day (24 h) , the total CO2 flux under effects of cyanobacteria crust was increased by 7.7% , while that under effects of moss crust was decreased by 29.6%, as compared with the total CO2 flux in bare land. This study suggested that in the hilly region of Loess Plateau, biocrust had significant effects on soil CO2 flux, which should be taken into consideration when assessing the carbon budget of the 'Grain for Green' eco-project.

Heat flux and quantum correlations in dissipative cascaded systems

Science.gov (United States)

Lorenzo, Salvatore; Farace, Alessandro; Ciccarello, Francesco; Palma, G. Massimo; Giovannetti, Vittorio

2015-02-01

We study the dynamics of heat flux in the thermalization process of a pair of identical quantum systems that interact dissipatively with a reservoir in a cascaded fashion. Despite that the open dynamics of the bipartite system S is globally Lindbladian, one of the subsystems "sees" the reservoir in a state modified by the interaction with the other subsystem and hence it undergoes a non-Markovian dynamics. As a consequence, the heat flow exhibits a nonexponential time behavior which can greatly deviate from the case where each party is independently coupled to the reservoir. We investigate both thermal and correlated initial states of S and show that the presence of correlations at the beginning can considerably affect the heat-flux rate. We carry out our study in two paradigmatic cases—a pair of harmonic oscillators with a reservoir of bosonic modes and two qubits with a reservoir of fermionic modes—and compare the corresponding behaviors. In the case of qubits and for initial thermal states, we find that the trace distance discord is at any time interpretable as the correlated contribution to the total heat flux.

Measurement of a surface heat flux and temperature

Science.gov (United States)

Davis, R. M.; Antoine, G. J.; Diller, T. E.; Wicks, A. L.

1994-04-01

The Heat Flux Microsensor is a new sensor which was recently patented by Virginia Tech and is just starting to be marketed by Vatell Corp. The sensor is made using the thin-film microfabrication techniques directly on the material that is to be measured. It consists of several thin-film layers forming a differential thermopile across a thermal resistance layer. The measured heat flux q is proportional to the temperature difference across the resistance layer q= k(sub g)/delta(sub g) x (t(sub 1) - T(sub 2)), where k(sub g) is the thermal conductivity and delta (sub g) is the thickness of the thermal resistance layer. Because the gages are sputter coated directly onto the surface, their total thickness is less than 2 micrometers, which is two orders of magnitude thinner than previous gages. The resulting temperature difference across the thermal resistance layer (delta is less than 1 micrometer) is very small even at high heat fluxes. To generate a measurable signal many thermocouple pairs are put in series to form a differential thermopile. The combination of series thermocouple junctions and thin-film design creates a gage with very attractive characteristics. It is not only physically non-intrusive to the flow, but also causes minimal disruption of the surface temperature. Because it is so thin, the response time is less than 20 microsec. Consequently, the frequency response is flat from 0 to over 50 kHz. Moreover, the signal of the Heat Flux Microsensor is directly proportional to the heat flux. Therefore, it can easily be used in both steady and transient flows, and it measures both the steady and unsteady components of the surface heat flux. A version of the Heat Flux Microsensor has been developed to meet the harsh demands of combustion environments. These gages use platinum and platinum-10 percent rhodium as the thermoelectric materials. The thermal resistance layer is silicon monoxide and a protective coating of Al2O3 is deposited on top of the sensor. The

Design Optimization for a Maglev System Employing Flux Eliminating Coils

Science.gov (United States)

Davey, Kent R.

1996-01-01

Flux eliminating coils have received no little attention over the past thirty years as an alternative for realizing lift in a MAGLEV system. When the magnets on board the vehicle are displaced from the equilibrium or null flux point of these coils, they induce current in those coils which act to restore the coil to its null flux or centerline position. The question being addressed in this paper is that of how to choose the best coil for a given system. What appears at first glance to be an innocent question is in fact one that is actually quite involved, encompassing both the global economics and physics of the system. The real key in analyzing that question is to derive an optimization index or functional which represents the cost of the system subject to constraints, the primary constraint being that the vehicle lift itself at a certain threshold speed. Outlined in this paper is one scenario for realizing a total system design which uses sequential quadratic programming techniques.

Neutron flux monitoring device

International Nuclear Information System (INIS)

Shimazu, Yoichiro.

1995-01-01

In a neutron flux monitoring device, there are disposed a neutron flux measuring means for outputting signals in accordance with the intensity of neutron fluxes, a calculation means for calculating a self power density spectrum at a frequency band suitable to an object to be measured based on the output of the neutron flux measuring means, an alarm set value generation means for outputting an alarm set value as a comparative reference, and an alarm judging means for comparing the alarm set value with the outputted value of the calculation means to judge requirement of generating an alarm and generate an alarm in accordance with the result of the judgement. Namely, the time-series of neutron flux signals is put to fourier transformation for a predetermined period of time by the calculation means, and from each of square sums for real number component and imaginary number component for each of the frequencies, a self power density spectrum in the frequency band suitable to the object to be measured is calculated. Then, when the set reference value is exceeded, an alarm is generated. This can reliably prevent generation of erroneous alarm due to neutron flux noises and can accurately generate an alarm at an appropriate time. (N.H.)

Changing trends of rainfall and sediment fluxes in the Kinta River catchment, Malaysia

Directory of Open Access Journals (Sweden)

W. R. Ismail

2015-03-01

Full Text Available The Kinta River, draining an area of 2566 km2, originates in the Korbu Mountain in Perak, Malaysia, and flows through heterogeneous, mixed land uses ranging from extensive forests to mining, rubber and oil palm plantations, and urban development. A land use change analysis of the Kinta River catchment was carried out together with assessment of the long-term trend in rainfall and sediment fluxes. The Mann-Kendall test was used to examine and assess the long-term trends in rainfall and its relationship with the sediment discharge trend. The land use analysis shows that forests, water bodies and mining land declined whilst built and agricultural land use increased significantly. This has influenced the sediment flux of the catchment. However, most of the rainfall stations and river gauging stations are experiencing an increasing trends, except at Kinta river at Tg. Rambutan. Sediment flux shows a net erosion for the period from 1961 to 1969. The total annual sediment discharge in the Kinta River catchment was low with an average rate of 1,757 t/km2/year. From 1970 to 1985, the annual sediment yield rose to an average rate of 4062 t/km2/year. Afterwards, from 1986 to 1993, the total annual sediment discharge decreased to an average rate of 1,306 t/km2/year and increased back during the period 1994 to 2000 to 2109 t/km2/year. From 2001 to 2006 the average sediment flux rate declined to 865 t/km2/year. The decline was almost 80% from the 1970s. High sediment flux in the early 1970s is partly associated with reduced tin mining activities in the area. This decreasing trend in sediment delivery leaving the Kinta River catchment is expected to continue dropping in the future.

Changing trends of rainfall and sediment fluxes in the Kinta River catchment, Malaysia

Science.gov (United States)

Ismail, W. R.; Hashim, M.

2015-03-01

The Kinta River, draining an area of 2566 km2, originates in the Korbu Mountain in Perak, Malaysia, and flows through heterogeneous, mixed land uses ranging from extensive forests to mining, rubber and oil palm plantations, and urban development. A land use change analysis of the Kinta River catchment was carried out together with assessment of the long-term trend in rainfall and sediment fluxes. The Mann-Kendall test was used to examine and assess the long-term trends in rainfall and its relationship with the sediment discharge trend. The land use analysis shows that forests, water bodies and mining land declined whilst built and agricultural land use increased significantly. This has influenced the sediment flux of the catchment. However, most of the rainfall stations and river gauging stations are experiencing an increasing trends, except at Kinta river at Tg. Rambutan. Sediment flux shows a net erosion for the period from 1961 to 1969. The total annual sediment discharge in the Kinta River catchment was low with an average rate of 1,757 t/km2/year. From 1970 to 1985, the annual sediment yield rose to an average rate of 4062 t/km2/year. Afterwards, from 1986 to 1993, the total annual sediment discharge decreased to an average rate of 1,306 t/km2/year and increased back during the period 1994 to 2000 to 2109 t/km2/year. From 2001 to 2006 the average sediment flux rate declined to 865 t/km2/year. The decline was almost 80% from the 1970s. High sediment flux in the early 1970s is partly associated with reduced tin mining activities in the area. This decreasing trend in sediment delivery leaving the Kinta River catchment is expected to continue dropping in the future.

Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau

Science.gov (United States)

Liu, Yang; Yan, Caiyu; Matthew, Cory; Wood, Brennon; Hou, Fujiang

2017-01-01

Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key seasons in 2012 and 2013 from a range of potential sources, including: alpine meadows, dung patches, manure heaps and yak night pens, on the Qinghai-Tibetan Plateau. We also estimated the total annual global warming potential (GWP, CO2-equivalents) from family farm grazing yaks using our measured results and other published data. In this study, GHG fluxes per unit area from night pens and composting manure heaps were higher than from dung patches and alpine meadows. Increased moisture content and surface temperature of soil and manure were major factors increasing CO2 and CH4 fluxes. High contributions of CH4 and N2O (21.1% and 44.8%, respectively) to the annual total GWP budget (334.2 tonnes) strongly suggest these GHG other than CO2 should not be ignored when estimating GWP from the family farm grazing yaks on the Qinghai-Tibetan Plateau for the purposes of determining national and regional land use policies or compiling global GHG inventories.

Decline of Phosphotransfer and Substrate Supply Metabolic Circuits Hinders ATP Cycling in Aging Myocardium.

Directory of Open Access Journals (Sweden)

Emirhan Nemutlu

Full Text Available Integration of mitochondria with cytosolic ATP-consuming/ATP-sensing and substrate supply processes is critical for muscle bioenergetics and electrical activity. Whether age-dependent muscle weakness and increased electrical instability depends on perturbations in cellular energetic circuits is unknown. To define energetic remodeling of aged atrial myocardium we tracked dynamics of ATP synthesis-utilization, substrate supply, and phosphotransfer circuits through adenylate kinase (AK, creatine kinase (CK, and glycolytic/glycogenolytic pathways using 18O stable isotope-based phosphometabolomic technology. Samples of intact atrial myocardium from adult and aged rats were subjected to 18O-labeling procedure at resting basal state, and analyzed using the 18O-assisted HPLC-GC/MS technique. Characteristics for aging atria were lower inorganic phosphate Pi[18O], γ-ATP[18O], β-ADP[18O], and creatine phosphate CrP[18O] 18O-labeling rates indicating diminished ATP utilization-synthesis and AK and CK phosphotransfer fluxes. Shift in dynamics of glycolytic phosphotransfer was reflected in the diminished G6P[18O] turnover with relatively constant glycogenolytic flux or G1P[18O] 18O-labeling. Labeling of G3P[18O], an indicator of G3P-shuttle activity and substrate supply to mitochondria, was depressed in aged myocardium. Aged atrial myocardium displayed reduced incorporation of 18O into second (18O2, third (18O3, and fourth (18O4 positions of Pi[18O] and a lower Pi[18O]/γ-ATP[18 O]-labeling ratio, indicating delayed energetic communication and ATP cycling between mitochondria and cellular ATPases. Adrenergic stress alleviated diminished CK flux, AK catalyzed β-ATP turnover and energetic communication in aging atria. Thus, 18O-assisted phosphometabolomics uncovered simultaneous phosphotransfer through AK, CK, and glycolytic pathways and G3P substrate shuttle deficits hindering energetic communication and ATP cycling, which may underlie energetic

The Open Flux Problem

Science.gov (United States)

Linker, J. A.; Caplan, R. M.; Downs, C.; Riley, P.; Mikic, Z.; Lionello, R.; Henney, C. J.; Arge, C. N.; Liu, Y.; Derosa, M. L.; Yeates, A.; Owens, M. J.

2017-10-01

The heliospheric magnetic field is of pivotal importance in solar and space physics. The field is rooted in the Sun’s photosphere, where it has been observed for many years. Global maps of the solar magnetic field based on full-disk magnetograms are commonly used as boundary conditions for coronal and solar wind models. Two primary observational constraints on the models are (1) the open field regions in the model should approximately correspond to coronal holes (CHs) observed in emission and (2) the magnitude of the open magnetic flux in the model should match that inferred from in situ spacecraft measurements. In this study, we calculate both magnetohydrodynamic and potential field source surface solutions using 14 different magnetic maps produced from five different types of observatory magnetograms, for the time period surrounding 2010 July. We have found that for all of the model/map combinations, models that have CH areas close to observations underestimate the interplanetary magnetic flux, or, conversely, for models to match the interplanetary flux, the modeled open field regions are larger than CHs observed in EUV emission. In an alternative approach, we estimate the open magnetic flux entirely from solar observations by combining automatically detected CHs for Carrington rotation 2098 with observatory synoptic magnetic maps. This approach also underestimates the interplanetary magnetic flux. Our results imply that either typical observatory maps underestimate the Sun’s magnetic flux, or a significant portion of the open magnetic flux is not rooted in regions that are obviously dark in EUV and X-ray emission.

The Open Flux Problem

Energy Technology Data Exchange (ETDEWEB)

Linker, J. A.; Caplan, R. M.; Downs, C.; Riley, P.; Mikic, Z.; Lionello, R. [Predictive Science Inc., 9990 Mesa Rim Road, Suite 170, San Diego, CA 92121 (United States); Henney, C. J. [Air Force Research Lab/Space Vehicles Directorate, 3550 Aberdeen Avenue SE, Kirtland AFB, NM (United States); Arge, C. N. [Science and Exploration Directorate, NASA/GSFC, Greenbelt, MD 20771 (United States); Liu, Y. [W. W. Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA 94305 (United States); Derosa, M. L. [Lockheed Martin Solar and Astrophysics Laboratory, 3251 Hanover Street B/252, Palo Alto, CA 94304 (United States); Yeates, A. [Department of Mathematical Sciences, Durham University, Durham, DH1 3LE (United Kingdom); Owens, M. J., E-mail: linkerj@predsci.com [Space and Atmospheric Electricity Group, Department of Meteorology, University of Reading, Earley Gate, P.O. Box 243, Reading RG6 6BB (United Kingdom)

2017-10-10

The heliospheric magnetic field is of pivotal importance in solar and space physics. The field is rooted in the Sun’s photosphere, where it has been observed for many years. Global maps of the solar magnetic field based on full-disk magnetograms are commonly used as boundary conditions for coronal and solar wind models. Two primary observational constraints on the models are (1) the open field regions in the model should approximately correspond to coronal holes (CHs) observed in emission and (2) the magnitude of the open magnetic flux in the model should match that inferred from in situ spacecraft measurements. In this study, we calculate both magnetohydrodynamic and potential field source surface solutions using 14 different magnetic maps produced from five different types of observatory magnetograms, for the time period surrounding 2010 July. We have found that for all of the model/map combinations, models that have CH areas close to observations underestimate the interplanetary magnetic flux, or, conversely, for models to match the interplanetary flux, the modeled open field regions are larger than CHs observed in EUV emission. In an alternative approach, we estimate the open magnetic flux entirely from solar observations by combining automatically detected CHs for Carrington rotation 2098 with observatory synoptic magnetic maps. This approach also underestimates the interplanetary magnetic flux. Our results imply that either typical observatory maps underestimate the Sun’s magnetic flux, or a significant portion of the open magnetic flux is not rooted in regions that are obviously dark in EUV and X-ray emission.

Modelling of interplanetary pickup ion fluxes and relevance for LISM parameters

International Nuclear Information System (INIS)

Fahr, H.J.; Rucinski, D.

1989-01-01

It has been known for many years that neutral interstellar atoms enter the solar system from the upwind side and penetrate deep into the inner heliosphere. Helium atoms, in particular, advance towards very small solar distances before they are ionized and then again convected as He - pickup ions outwards with the solar wind. Since these ions were recently detected in space, we concentrate here on calculations of He + production rates and He + fluxes. It is shown that inside 1 a.u., the He - production is essentially determined both by solar e.u.v. photoionization and by electron impact ionization. We calculate He + production rates as a function of space coordinates, taking into account the core-halo structure of the energy distribution of solar wind electrons and their temperature distribution with distance according to relevant solar wind models. For this purpose, a newly developed program to compute He densities was used. In contrast to the production of H + , the He - production rates are found to be higher on the downwind axis than on the upwind axis by a factor of 5. We also determine partial and total He + ion fluxes as a function of solar distance and longitude. It is interesting to note that only the values for total fluxes agree well with the integrated He + fluxes measured by the SULEICA experiment aboard the AMPTE satellite. This indicates that pickup ions under the influence of the intrinsic MHD wave turbulence in the solar wind change their primary seed distribution function by rapid pitch-angle scattering and subsequent adiabatic cooling. To interpret the He + intensity profile along the orbit of the Earth in terms of LISM helium parameters, we point to the need to take into account carefully electron impact ionization in order to prevent misinterpretations. (author)

Characterization of ion fluxes and heat fluxes for PMI relevant conditions on Proto-MPEX

Science.gov (United States)

Beers, Clyde; Shaw, Guinevere; Biewer, Theodore; Rapp, Juergen

2016-10-01

Plasma characterization, in particular, particle flux and electron and ion temperature distributions nearest to an exposed target, are critical to quantifying Plasma Surface Interaction (PSI). In the Proto-Material Plasma Exposure eXperiment (Proto-MPEX), the ion fluxes and heat fluxes are derived from double Langmuir Probes (DLP) and Thomson Scattering in front of the target assuming Bohm conditions at the sheath entrance. Power fluxes derived from ne and Te measurements are compared to heat fluxes measured with IR thermography. The comparison will allow conclusions on the sheath heat transmission coefficient to be made experimentally. Different experimental conditions (low and high density plasmas (0.5 - 6 x 1019 m-3) with different magnetic configuration are compared. This work was supported by the U.S. D.O.E. contract DE-AC05-00OR22725.

Flux

DEFF Research Database (Denmark)

Ravn, Ib

. FLUX betegner en flyden eller strømmen, dvs. dynamik. Forstår man livet som proces og udvikling i stedet for som ting og mekanik, får man et andet billede af det gode liv end det, som den velkendte vestlige mekanicisme lægger op til. Dynamisk forstået indebærer det gode liv den bedst mulige...... kanalisering af den flux eller energi, der strømmer igennem os og giver sig til kende i vore daglige aktiviteter. Skal vores tanker, handlinger, arbejde, samvær og politiske liv organiseres efter stramme og faste regelsæt, uden slinger i valsen? Eller skal de tværtimod forløbe ganske uhindret af regler og bånd...

Low energy neutral particle fluxes in the JET divertor

International Nuclear Information System (INIS)

Reichle, R.; Horton, L.D.; Ingesson, L.C.; Jaeckel, H.J.; McCormick, G.K.; Loarte, A.; Simonini, R.; Stamp, M.F.

1997-01-01

First measurements are presented of the total power loss through neutral particles and their average energy in the JET divertor. The method used distinguishes between the heat flux and the electromagnetic radiation on bolometers. This is done by comparing measurements from inside the divertor either with opposite lines of sight or with a tomographic reconstruction of the radiation. The typical value of the total power loss in the divertor through neutrals is about 1 MW. The average energy of the neutral particles at the inner divertor leg is 1.5-3 eV when detachment is in progress, which agrees with EDGE2D/NIMBUS modelling. (orig.)

COSANI-2, Gamma Doses from SABINE Calculation, Activity from ANISN Flux Calculation

International Nuclear Information System (INIS)

Dupont, C.

1975-01-01

1 - Nature of physical problem solved: Retrieval of SABINE and/or ANISN results. Calculates in case of SABINE results the individual contributions of capture gamma rays in each region to the total gamma dose and to the total gamma heating may calculate in case of ANISN new activity rates starting from ANISN flux saved on tape and activity cross sections taken on an ANISN binary library tape. The program can draw on a BENSON plotter any of the following quantities: - group flux; - activity rates; - dose rates; - neutron spectra for SABINE; - neutron or gamma direct or adjoint spectra for ANISN; - gamma heating and dose rate for SABINE including individual contributions from each region. Several ANISN and/or SABINE cases can be drawn on the same graph for comparison purposes. 2 - Restrictions on the complexity of the problem: Maximum number of: - tapes containing ANISN and/or SABINE results: 5; - curves per graph: 3; - regions: 40; - points per curve: 500; - energy groups: 200

Consistency of lattice definitions of U(1) flux in Abelian projected SU(2) gauge theory

International Nuclear Information System (INIS)

Matsuki, Takayuki; Haymaker, Richard W.

2004-01-01

We reexamine the dual Abrikosov vortex under the requirement that the lattice averages of the fields satisfy exact Maxwell equations [ME]. The electric ME accounts for the total flux and the magnetic ME determines the shape of the confining string. This leads to unique and consistent definitions of flux and electric and magnetic currents at finite lattice spacing. The resulting modification of the standard DeGrand-Toussaint construction gives a magnetic current comprised of smeared monopoles

FluxPyt: a Python-based free and open-source software for 13C-metabolic flux analyses.

Science.gov (United States)

Desai, Trunil S; Srivastava, Shireesh

2018-01-01

13 C-Metabolic flux analysis (MFA) is a powerful approach to estimate intracellular reaction rates which could be used in strain analysis and design. Processing and analysis of labeling data for calculation of fluxes and associated statistics is an essential part of MFA. However, various software currently available for data analysis employ proprietary platforms and thus limit accessibility. We developed FluxPyt, a Python-based truly open-source software package for conducting stationary 13 C-MFA data analysis. The software is based on the efficient elementary metabolite unit framework. The standard deviations in the calculated fluxes are estimated using the Monte-Carlo analysis. FluxPyt also automatically creates flux maps based on a template for visualization of the MFA results. The flux distributions calculated by FluxPyt for two separate models: a small tricarboxylic acid cycle model and a larger Corynebacterium glutamicum model, were found to be in good agreement with those calculated by a previously published software. FluxPyt was tested in Microsoft™ Windows 7 and 10, as well as in Linux Mint 18.2. The availability of a free and open 13 C-MFA software that works in various operating systems will enable more researchers to perform 13 C-MFA and to further modify and develop the package.

Sedimentary record of water column trophic conditions and sediment carbon fluxes in a tropical water reservoir (Valle de Bravo, Mexico).

Science.gov (United States)

Carnero-Bravo, Vladislav; Merino-Ibarra, Martín; Ruiz-Fernández, Ana Carolina; Sanchez-Cabeza, Joan Albert; Ghaleb, Bassam

2015-03-01

Valle de Bravo (VB) is the main water reservoir of the Cutzamala hydraulic system, which provides 40% of the drinking water consumed in the Mexico City Metropolitan Area and exhibits symptoms of eutrophication. Nutrient (C, N and P) concentrations were determined in two sediment cores to reconstruct the water column trophic evolution of the reservoir and C fluxes since its creation in 1947. Radiometric methods ((210)Pb and (137)Cs) were used to obtain sediment chronologies, using the presence of pre-reservoir soil layers in one of the cores as an independent chronological marker. Mass accumulation rates ranged from 0.12 to 0.56 g cm(-2) year(-1) and total organic carbon (TOC) fluxes from 122 to 380 g m(-2) year(-1). Total N ranged 4.9-48 g m(-2) year(-1), and total P 0.6-4.2 g m(-2) year(-1). The sedimentary record shows that all three (C, N and P) fluxes increased significantly after 1991, in good agreement with the assessed trophic evolution of VB and with historic and recent real-time measurements. In the recent years (1992-2006), the TOC flux to the bottom of VB (average 250 g m(-2) year(-1), peaks 323 g m(-2) year(-1)) is similar to that found in highly eutrophic reservoirs and impoundments. Over 1/3 of the total C burial since dam construction, circa 70,000 t, has occurred in this recent period. These results highlight the usefulness of the reconstruction of carbon and nutrient fluxes from the sedimentary record to assess carbon burial and its temporal evolution in freshwater ecosystems.

Fluxes of oxidised and reduced nitrogen above a mixed coniferous forest exposed to various nitrogen emission sources

International Nuclear Information System (INIS)

Neirynck, J.; Kowalski, A.S.; Carrara, A.; Genouw, G.; Berghmans, P.; Ceulemans, R.

2007-01-01

Concentrations of nitrogen gases (NH 3 , NO 2 , NO, HONO and HNO 3 ) and particles (pNH 4 and pNO 3 ) were measured over a mixed coniferous forest impacted by high nitrogen loads. Nitrogen dioxide (NO 2 ) represented the main nitrogen form, followed by nitric oxide (NO) and ammonia (NH 3 ). A combination of gradient method (NH 3 and NO x ) and resistance modelling techniques (HNO 3 , HONO, pNH 4 and pNO 3 ) was used to calculate dry deposition of nitrogen compounds. Net flux of NH 3 amounted to -64 ng N m -2 s -1 over the measuring period. Net fluxes of NO x were upward (8.5 ng N m -2 s -1 ) with highest emission in the morning. Fluxes of other gases or aerosols substantially contributed to dry deposition. Total nitrogen deposition was estimated at -48 kg N ha -1 yr -1 and consisted for almost 80% of NH x . Comparison of throughfall nitrogen with total deposition suggested substantial uptake of reduced N (±15 kg N ha -1 yr -1 ) within the canopy. - Reduced nitrogen was found to be the main contributor to total deposition which was predominantly governed by dry deposition

Particle flux on the continental shelf in the Amundsen Sea Polynya and Western Antarctic Peninsula

Directory of Open Access Journals (Sweden)

Hugh W. Ducklow

2015-04-01

Full Text Available Abstract We report results from a yearlong, moored sediment trap in the Amundsen Sea Polynya (ASP, the first such time series in this remote and productive ecosystem. Results are compared to a long-term (1992–2013 time series from the western Antarctic Peninsula (WAP. The ASP trap was deployed from December 2010 to December 2011 at 350 m depth. We observed two brief, but high flux events, peaking at 8 and 5 mmol C m−2 d−1 in January and December 2011, respectively, with a total annual capture of 315 mmol C m−2. Both peak fluxes and annual capture exceeded the comparable WAP observations. Like the overlying phytoplankton bloom observed during the cruise in the ASP (December 2010 to January 2011, particle flux was dominated by Phaeocystis antarctica, which produced phytodetrital aggregates. Particles at the start of the bloom were highly depleted in 13C, indicating their origin in the cold, CO2-rich winter waters exposed by retreating sea ice. As the bloom progressed, microscope visualization and stable isotopic composition provided evidence for an increasing contribution by zooplankton fecal material. Incubation experiments and zooplankton observations suggested that fecal pellet production likely contributed 10–40% of the total flux during the first flux event, and could be very high during episodic krill swarms. Independent estimates of export from the surface (100 m were about 5–10 times that captured in the trap at 350 m. Estimated bacterial respiration was sufficient to account for much of the decline in the flux between 50 and 350 m, whereas zooplankton respiration was much lower. The ASP system appears to export only a small fraction of its production deeper than 350 m within the polynya region. The export efficiency was comparable to other polar regions where phytoplankton blooms were not dominated by diatoms.

Topology of magnetic flux ropes and formation of fossil flux transfer events and boundary layer plasmas

Science.gov (United States)

Lee, L. C.; Ma, Z. W.; Fu, Z. F.; Otto, A.

1993-01-01

A mechanism for the formation of fossil flux transfer events and the low-level boundary layer within the framework of multiple X-line reconnection is proposed. Attention is given to conditions for which the bulk of magnetic flux in a flux rope of finite extent has a simple magnetic topology, where the four possible connections of magnetic field lines are: IMF to MSP, MSP to IMF, IMF to IMF, and MSP to MSP. For a sufficient relative shift of the X lines, magnetic flux may enter a flux rope from the magnetosphere and exit into the magnetosphere. This process leads to the formation of magnetic flux ropes which contain a considerable amount of magnetosheath plasma on closed magnetospheric field lines. This process is discussed as a possible explanation for the formation of fossil flux transfer events in the magnetosphere and the formation of the low-latitude boundary layer.

Total variation-based neutron computed tomography

Science.gov (United States)

Barnard, Richard C.; Bilheux, Hassina; Toops, Todd; Nafziger, Eric; Finney, Charles; Splitter, Derek; Archibald, Rick

2018-05-01

We perform the neutron computed tomography reconstruction problem via an inverse problem formulation with a total variation penalty. In the case of highly under-resolved angular measurements, the total variation penalty suppresses high-frequency artifacts which appear in filtered back projections. In order to efficiently compute solutions for this problem, we implement a variation of the split Bregman algorithm; due to the error-forgetting nature of the algorithm, the computational cost of updating can be significantly reduced via very inexact approximate linear solvers. We present the effectiveness of the algorithm in the significantly low-angular sampling case using synthetic test problems as well as data obtained from a high flux neutron source. The algorithm removes artifacts and can even roughly capture small features when an extremely low number of angles are used.

Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities

Directory of Open Access Journals (Sweden)

Martinez Alfredo

2008-05-01

Full Text Available Abstract Background A metabolic regulation study was performed, based upon measurements of enzymatic activities, fermentation performance, and RT-PCR analysis of pathways related to central carbon metabolism, in an ethanologenic Escherichia coli strain (CCE14 derived from lineage C. In comparison with previous engineered strains, this E coli derivative has a higher ethanol production rate in mineral medium, as a result of the elevated heterologous expression of the chromosomally integrated genes encoding PDCZm and ADHZm (pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis. It is suggested that this behavior might be due to lineage differences between E. coli W and C. Results This study demonstrated that the glycolytic flux is controlled, in this case, by reactions outside glycolysis, i.e., the fermentative pathways. Changes in ethanol production rate in this ethanologenic strain result in low organic acid production rates, and high glycolytic and ethanologenic fluxes, that correlate with enhanced transcription and enzymatic activity levels of PDCZm and ADHZm. Furthermore, a higher ethanol yield (90% of the theoretical in glucose-mineral media was obtained with CCE14 in comparison with previous engineered E. coli strains, such as KO11, that produces a 70% yield under the same conditions. Conclusion Results suggest that a higher ethanol formation rate, caused by ahigher PDCZm and ADHZm activities induces a metabolic state that cells compensate through enhanced glucose transport, ATP synthesis, and NAD-NADH+H turnover rates. These results show that glycolytic enzymatic activities, present in E. coli W and C under fermentative conditions, are sufficient to contend with increases in glucose consumption and product formation rates.

Comparisons between POC and zooplankton swimmer flux from sediment traps in the subarctic and subtropical North Pacific

Science.gov (United States)

Yokoi, Naoya; Abe, Yoshiyuki; Kitamura, Minoru; Honda, Makio C.; Yamaguchi, Atsushi

2018-03-01

Seasonal changes in zooplankton swimmer (ZS) abundance, biomass and community structure were evaluated based on samples collected by moored sediment traps at a depth of 200 m in the subarctic (SA) and subtropical (ST) western North Pacific. Based on these samples, we made comparisons on two topics: 1) latitudinal (subarctic vs. subtropical) changes in ZS abundance, biomass and community and 2) quantitative differences between the ZS and particle organic carbon (POC) fluxes based on data from moored or drifting sediment traps. The results showed that the ZS flux was greater in the SA (annual mean: 311 ind. m-2 day-1 or 258 mg C m-2 day-1) than in the ST (135 ind. m-2 day-1 or 38 mg C m-2 day-1). The peak ZS flux was observed from July-August in the SA and from April-May in the ST. The dominant taxa were Copepoda and Chaetognatha in the SA and Ostracoda and Mollusca in the ST. These latitudinal differences are likely related to the dominance of large-sized Copepoda in the SA, regional differences in the timing of the spring phytoplankton bloom, and the magnitude and size structure of primary producers. The percent composition of ZS to the total C flux (= ZS+POC flux) varied by region: 85-95% in the SA and 47-75% in the ST. These differences between the ZS composition and the total C flux are most likely caused by the dominance of large-sized Copepoda (Neocalanus spp. and Eucalanus bungii) in the SA.

Reluctance motor employing superconducting magnetic flux switches

International Nuclear Information System (INIS)

Spyker, R.L.; Ruckstadter, E.J.

1992-01-01

This paper reports that superconducting flux switches controlling the magnetic flux in the poles of a motor will enable the implementation of a reluctance motor using one central single phase winding. A superconducting flux switch consists of a ring of superconducting material surrounding a ferromagnetic pole of the motor. When in the superconducting state the switch will block all magnetic flux attempting to flow in the ferromagnetic core. When switched to the normal state the superconducting switch will allow the magnetic flux to flow freely in that pole. By using one high turns-count coil as a flux generator, and selectively channeling flux among the various poles using the superconducting flux switch, 3-phase operation can be emulated with a single-hase central AC source. The motor will also operate when the flux generating coil is driven by a DC current, provided the magnetic flux switches see a continuously varying magnetic flux. Rotor rotation provides this varying flux due to the change in stator pole inductance it produces

The Oceanic Flux Program: A three decade time-series of particle flux in the deep Sargasso Sea

Science.gov (United States)

Weber, J. C.; Conte, M. H.

2010-12-01

The Oceanic Flux Program (OFP), 75 km SE of Bermuda, is the longest running time-series of its kind. Initiated in 1978, the OFP has produced an unsurpassed, nearly continuous record of temporal variability in deep ocean fluxes, with a >90% temporal coverage at 3200m depth. The OFP, in conjunction with the co-located Bermuda-Atlantic Time Series (BATS) and the Bermuda Testbed Mooring (BTM) time-series, has provided key observations enabling detailed assessment of how seasonal and non-seasonal variability in the deep ocean is linked with the overlying physical and biogeochemical environment. This talk will focus on the short-term flux variability that overlies the seasonal flux pattern in the Sargasso Sea, emphasizing episodic extreme flux events. Extreme flux events are responsible for much of the year-to-year variability in mean annual flux and are most often observed during early winter and late spring when surface stratification is weak or transient. In addition to biological phenomena (e.g. salp blooms), passage of productive meso-scale features such as eddies, which alter surface water mixing characteristics and surface export fluxes, may initiate some extreme flux events. Yet other productive eddies show a minimal influence on the deep flux, underscoring the importance of upper ocean ecosystem structure and midwater processes on the coupling between the surface ocean environment and deep fluxes. Using key organic and inorganic tracers, causative processes that influence deep flux generation and the strength of the coupling with the surface ocean environment can be identified.

Flux-pinning-induced stresses in a hollow superconducting cylinder with flux creep and viscosity properties

International Nuclear Information System (INIS)

Feng, W.J.; Gao, S.W.

2014-01-01

Highlights: • Magnetoelastic problem for a superconducting cylinder with a hole is investigated. • The effects of both flux creep and viscous flux flow on stresses are analyzed. • For the FC case, the maximal hoop tensile stress always occurs at hole edge. • For the ZFC case, the maximal hoop stress is not certain to occur at hole edge. - Abstract: The magnetoelastic problem for a superconducting cylinder with a concentric hole placed in a magnetic field is investigated, where the flux creep and viscous flux flow have been considered. The stress distributions are derived and numerical calculated for the descending field in both the zero-field cooling (ZFC) and field cooling (FC) processes. The effects of applied magnetic field, flux creep and viscous flux flow on the maximal radial and hoop stresses are discussed in detail, and some novel phenomena are found. Among others, for the FC case, the maximal hoop tensile stress always occurs at the hole edge, whist for the ZFC case, the maximal stresses including both hoop and radial stresses either occur in the vicinity of the hole or occur at the position of flux frontier in the remagnetization process. For the descending field, in general, both the flux creep and viscosity parameters have important effects on the maximal radial and hoop stresses. All these phenomena are perhaps of vital importance for the application of superconductors

Forty-year trends in the flux and concentration of phosphorus in British rivers

Science.gov (United States)

Civan, Aylin; Worrall, Fred; Jarvie, Helen P.; Howden, Nicholas J. K.; Burt, Tim P.

2018-03-01

Given the importance of phosphorus (P) in the eutrophication of natural waters, this study considered the long-term time series of total phosphorus (TP) and total reactive phosphorus (TRP) in British rivers from 1974 to 2012. The approach included not only trend analysis of fluxes and concentrations but also change point analysis. TP and TRP concentrations and fluxes in British rivers have declined since the mid-1980s. Over the last decade of the record the majority of individual sites did show significant downward trends in TP and TRP concentrations but, in 28% of cases for TRP concentration and 14% of cases for TP concentration, the decadal trend was a significant increase. Out of 230 sites, 136 showed a significant step decrease in TRP concentration; no sites showed a significant step increase. The modal year for the step changes for both TRP concentration and flux was 1997. Step changes are likely associated with improvements made at sewage treatment works to comply with the Urban Waste Water Treatment Directive (91/271/EEC). The decrease in TRP concentration due to the step change were in the range of 0.68% and 89% with a geometric mean of 22%, with the rest of the decrease accounted by long-term, persistent downward trend.

Comparison of two regression-based approaches for determining nutrient and sediment fluxes and trends in the Chesapeake Bay watershed

Science.gov (United States)

Moyer, Douglas; Hirsch, Robert M.; Hyer, Kenneth

2012-01-01

Nutrient and sediment fluxes and changes in fluxes over time are key indicators that water resource managers can use to assess the progress being made in improving the structure and function of the Chesapeake Bay ecosystem. The U.S. Geological Survey collects annual nutrient (nitrogen and phosphorus) and sediment flux data and computes trends that describe the extent to which water-quality conditions are changing within the major Chesapeake Bay tributaries. Two regression-based approaches were compared for estimating annual nutrient and sediment fluxes and for characterizing how these annual fluxes are changing over time. The two regression models compared are the traditionally used ESTIMATOR and the newly developed Weighted Regression on Time, Discharge, and Season (WRTDS). The model comparison focused on answering three questions: (1) What are the differences between the functional form and construction of each model? (2) Which model produces estimates of flux with the greatest accuracy and least amount of bias? (3) How different would the historical estimates of annual flux be if WRTDS had been used instead of ESTIMATOR? One additional point of comparison between the two models is how each model determines trends in annual flux once the year-to-year variations in discharge have been determined. All comparisons were made using total nitrogen, nitrate, total phosphorus, orthophosphorus, and suspended-sediment concentration data collected at the nine U.S. Geological Survey River Input Monitoring stations located on the Susquehanna, Potomac, James, Rappahannock, Appomattox, Pamunkey, Mattaponi, Patuxent, and Choptank Rivers in the Chesapeake Bay watershed. Two model characteristics that uniquely distinguish ESTIMATOR and WRTDS are the fundamental model form and the determination of model coefficients. ESTIMATOR and WRTDS both predict water-quality constituent concentration by developing a linear relation between the natural logarithm of observed constituent

Dry-out heat fluxes of falling film and low-mass flux upward-flow in heated tubes

International Nuclear Information System (INIS)

Koizumi, Yasuo; Ueda, Tatsuhiro; Matsuo, Teruyuki; Miyota, Yukio

1998-01-01

Dry-out heat fluxes were investigated experimentally for a film flow falling down on the inner surface of vertical heated-tubes and for a low mass flux forced-upward flow in the tubes using R 113. This work followed the study on those for a two-phase natural circulation system. For the falling film boiling, flow state observation tests were also performed, where dry-patches appearing and disappearing repeatedly were observed near the exit end of the heated section at the dry-out heat flux conditions. Relation between the dry-out heat flux and the liquid film flow rate is analyzed. The dry-out heat fluxes of the low mass flux upflow are expressed well by the correlation proposed in the previous work. The relation for the falling film boiling shows a similar trend to that for the upflow boiling, however, the dry-out heat fluxes of the falling film are much lower, approximately one third, than those of the upward flow. (author)

Effects of High-Flux versus Low-Flux Membranes on Pulmonary Function Tests in Hemodialysis Patients.

Science.gov (United States)

Momeni, Ali; Rouhi, Hamid; Kiani, Glareh; Amiri, Masoud

2013-01-01

Several studies have been carried out to evaluate the effects of dialysis on pulmonary function tests (PFT). Dialysis procedure may reduce lung volumes and capacities or cause hypoxia; however, to the best of our knowledge, there is no previous study evaluating the effects of membrane type (high flux vs. low flux) on PFT in these patients. The aim of this study was the evaluation of this relationship. In this cross-sectional study, 43 hemodialysis patients without pulmonary disease were enrolled. In these patients dialysis was conducted by low-and high-flux membranes and before and after the procedure, spirometry was done and the results were evaluated by t-test and chi square test. The mean age of patients was 56.34 years. Twenty-three of them were female (53.5%). Type of membrane (high flux vs. low flux) had no effect on spirometry results of patients despite the significant decrease in the body weight during the dialysis session. High flux membrane had no advantage over low flux membrane in terms of improvement in spirometry findings; thus, we could not offer these expensive membranes for this purpose.

High Torque Density Transverse Flux Machine without the Need to Use SMC Material for 3D Flux Paths

DEFF Research Database (Denmark)

Lu, Kaiyuan; Wu, Weimin

2015-01-01

This paper presents a new transverse flux permanent magnet machine. In a normal transverse flux machine, complicated 3-D flux paths often exist. Such 3-D flux paths would require the use of soft magnetic composites material instead of laminations for construction of the machine stator. In the new...... machine topology proposed in this paper, by advantageously utilizing the magnetic flux path provided by an additional rotor, use of laminations that allow 2-D flux paths only will be sufficient to accomplish the required 3-D flux paths. The machine also has a high torque density and is therefore...

Subcellular localization of glycolytic enzymes and characterization of intermediary metabolism of Trypanosoma rangeli.

Science.gov (United States)

Rondón-Mercado, Rocío; Acosta, Héctor; Cáceres, Ana J; Quiñones, Wilfredo; Concepción, Juan Luis

2017-09-01

Trypanosoma rangeli is a hemoflagellate protist that infects wild and domestic mammals as well as humans in Central and South America. Although this parasite is not pathogenic for human, it is being studied because it shares with Trypanosoma cruzi, the etiological agent of Chagas' disease, biological characteristics, geographic distribution, vectors and vertebrate hosts. Several metabolic studies have been performed with T. cruzi epimastigotes, however little is known about the metabolism of T. rangeli. In this work we present the subcellular distribution of the T. rangeli enzymes responsible for the conversion of glucose to pyruvate, as determined by epifluorescense immunomicroscopy and subcellular fractionation involving either selective membrane permeabilization with digitonin or differential and isopycnic centrifugation. We found that in T. rangeli epimastigotes the first six enzymes of the glycolytic pathway, involved in the conversion of glucose to 1,3-bisphosphoglycerate are located within glycosomes, while the last four steps occur in the cytosol. In contrast with T. cruzi, where three isoenzymes (one cytosolic and two glycosomal) of phosphoglycerate kinase are expressed simultaneously, only one enzyme with this activity is detected in T. rangeli epimastigotes, in the cytosol. Consistent with this latter result, we found enzymes involved in auxiliary pathways to glycolysis needed to maintain adenine nucleotide and redox balances within glycosomes such as phosphoenolpyruvate carboxykinase, malate dehydrogenase, fumarate reductase, pyruvate phosphate dikinase and glycerol-3-phosphate dehydrogenase. Glucokinase, galactokinase and the first enzyme of the pentose-phosphate pathway, glucose-6-phosphate dehydrogenase, were also located inside glycosomes. Furthermore, we demonstrate that T. rangeli epimastigotes growing in LIT medium only consume glucose and do not excrete ammonium; moreover, they are unable to survive in partially-depleted glucose medium. The

Soil surface CO2 flux in a boreal black spruce fire chronosequence

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