Strain Elastography for Prediction of Malignancy in Soft Tissue Tumours--Preliminary Results

DEFF Research Database (Denmark)

Riishede, I; Ewertsen, C; Carlsen, J

2015-01-01

PURPOSE: To evaluate the ability of strain elastography to predict malignancy in patients with soft tissue tumors, and to compare three evaluation methods of strain elastography: strain ratios, strain histograms and visual scoring. MATERIALS AND METHODS: 60 patients with 61 tumors were analyzed...

Strain-induced collagen organization at the micro-level in fibrin-based engineered tissue constructs

NARCIS (Netherlands)

Jonge, de N.; Kanters, F.M.W.; Baaijens, F.P.T.; Bouten, C.V.C.

2013-01-01

Full understanding of strain-induced collagen organization in complex tissue geometries to create tissues with predefined collagen architecture has not been achieved. This is mainly due to our limited knowledge of collagen remodeling in developing tissues. Here we investigate strain-induced collagen

Strain modulations as a mechanism to reduce stress relaxation in laryngeal tissues.

Science.gov (United States)

Hunter, Eric J; Siegmund, Thomas; Chan, Roger W

2014-01-01

Vocal fold tissues in animal and human species undergo deformation processes at several types of loading rates: a slow strain involved in vocal fold posturing (on the order of 1 Hz or so), cyclic and faster posturing often found in speech tasks or vocal embellishment (1-10 Hz), and shear strain associated with vocal fold vibration during phonation (100 Hz and higher). Relevant to these deformation patterns are the viscous properties of laryngeal tissues, which exhibit non-linear stress relaxation and recovery. In the current study, a large strain time-dependent constitutive model of human vocal fold tissue is used to investigate effects of phonatory posturing cyclic strain in the range of 1 Hz to 10 Hz. Tissue data for two subjects are considered and used to contrast the potential effects of age. Results suggest that modulation frequency and extent (amplitude), as well as the amount of vocal fold overall strain, all affect the change in stress relaxation with modulation added. Generally, the vocal fold cover reduces the rate of relaxation while the opposite is true for the vocal ligament. Further, higher modulation frequencies appear to reduce the rate of relaxation, primarily affecting the ligament. The potential benefits of cyclic strain, often found in vibrato (around 5 Hz modulation) and intonational inflection, are discussed in terms of vocal effort and vocal pitch maintenance. Additionally, elderly tissue appears to not exhibit these benefits to modulation. The exacerbating effect such modulations may have on certain voice disorders, such as muscle tension dysphonia, are explored.

Strain modulations as a mechanism to reduce stress relaxation in laryngeal tissues.

Directory of Open Access Journals (Sweden)

Eric J Hunter

Full Text Available Vocal fold tissues in animal and human species undergo deformation processes at several types of loading rates: a slow strain involved in vocal fold posturing (on the order of 1 Hz or so, cyclic and faster posturing often found in speech tasks or vocal embellishment (1-10 Hz, and shear strain associated with vocal fold vibration during phonation (100 Hz and higher. Relevant to these deformation patterns are the viscous properties of laryngeal tissues, which exhibit non-linear stress relaxation and recovery. In the current study, a large strain time-dependent constitutive model of human vocal fold tissue is used to investigate effects of phonatory posturing cyclic strain in the range of 1 Hz to 10 Hz. Tissue data for two subjects are considered and used to contrast the potential effects of age. Results suggest that modulation frequency and extent (amplitude, as well as the amount of vocal fold overall strain, all affect the change in stress relaxation with modulation added. Generally, the vocal fold cover reduces the rate of relaxation while the opposite is true for the vocal ligament. Further, higher modulation frequencies appear to reduce the rate of relaxation, primarily affecting the ligament. The potential benefits of cyclic strain, often found in vibrato (around 5 Hz modulation and intonational inflection, are discussed in terms of vocal effort and vocal pitch maintenance. Additionally, elderly tissue appears to not exhibit these benefits to modulation. The exacerbating effect such modulations may have on certain voice disorders, such as muscle tension dysphonia, are explored.

A strain-hardening bi-power law for the nonlinear behaviour of biological soft tissues.

Science.gov (United States)

Nicolle, S; Vezin, P; Palierne, J-F

2010-03-22

Biological soft tissues exhibit a strongly nonlinear viscoelastic behaviour. Among parenchymous tissues, kidney and liver remain less studied than brain, and a first goal of this study is to report additional material properties of kidney and liver tissues in oscillatory shear and constant shear rate tests. Results show that the liver tissue is more compliant but more strain hardening than kidney. A wealth of multi-parameter mathematical models has been proposed for describing the mechanical behaviour of soft tissues. A second purpose of this work is to develop a new constitutive law capable of predicting our experimental data in the both linear and nonlinear viscoelastic regime with as few parameters as possible. We propose a nonlinear strain-hardening fractional derivative model in which six parameters allow fitting the viscoelastic behaviour of kidney and liver tissues for strains ranging from 0.01 to 1 and strain rates from 0.0151 s(-1) to 0.7s(-1). Copyright (c) 2009 Elsevier Ltd. All rights reserved.

Tissue strain rate estimator using ultrafast IQ complex data

OpenAIRE

TERNIFI , Redouane; Elkateb Hachemi , Melouka; Remenieras , Jean-Pierre

2012-01-01

International audience; Pulsatile motion of brain parenchyma results from cardiac and breathing cycles. In this study, transient motion of brain tissue was estimated using an Aixplorer® imaging system allowing an ultrafast 2D acquisition mode. The strain was computed directly from the ultrafast IQ complex data using the extended autocorrelation strain estimator (EASE), which provides great SNRs regardless of depth. The EASE first evaluates the autocorrelation function at each depth over a set...

Normal and Fibrotic Rat Livers Demonstrate Shear Strain Softening and Compression Stiffening: A Model for Soft Tissue Mechanics.

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Maryna Perepelyuk

Full Text Available Tissues including liver stiffen and acquire more extracellular matrix with fibrosis. The relationship between matrix content and stiffness, however, is non-linear, and stiffness is only one component of tissue mechanics. The mechanical response of tissues such as liver to physiological stresses is not well described, and models of tissue mechanics are limited. To better understand the mechanics of the normal and fibrotic rat liver, we carried out a series of studies using parallel plate rheometry, measuring the response to compressive, extensional, and shear strains. We found that the shear storage and loss moduli G' and G" and the apparent Young's moduli measured by uniaxial strain orthogonal to the shear direction increased markedly with both progressive fibrosis and increasing compression, that livers shear strain softened, and that significant increases in shear modulus with compressional stress occurred within a range consistent with increased sinusoidal pressures in liver disease. Proteoglycan content and integrin-matrix interactions were significant determinants of liver mechanics, particularly in compression. We propose a new non-linear constitutive model of the liver. A key feature of this model is that, while it assumes overall liver incompressibility, it takes into account water flow and solid phase compressibility. In sum, we report a detailed study of non-linear liver mechanics under physiological strains in the normal state, early fibrosis, and late fibrosis. We propose a constitutive model that captures compression stiffening, tension softening, and shear softening, and can be understood in terms of the cellular and matrix components of the liver.

Whole genome characterization of non-tissue culture adapted HRSV strains in severely infected children

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Kumaria Rajni

2011-07-01

Full Text Available Abstract Background Human respiratory syncytial virus (HRSV is the most important virus causing lower respiratory infection in young children. The complete genetic characterization of RSV clinical strains is a prerequisite for understanding HRSV infection in the clinical context. Current information about the genetic structure of the HRSV genome has largely been obtained using tissue culture adapted viruses. During tissue culture adaptation genetic changes can be introduced into the virus genome, which may obscure subtle variations in the genetic structure of different RSV strains. Methods In this study we describe a novel Sanger sequencing strategy which allowed the complete genetic characterisation of 14 clinical HRSV strains. The viruses were sequenced directly in the nasal washes of severely hospitalized children, and without prior passage of the viruses in tissue culture. Results The analysis of nucleotide sequences suggested that vRNA length is a variable factor among primary strains, while the phylogenetic analysis suggests selective pressure for change. The G gene showed the greatest sequence variation (2-6.4%, while small hydrophobic protein and matrix genes were completely conserved across all clinical strains studied. A number of sequence changes in the F, L, M2-1 and M2-2 genes were observed that have not been described in laboratory isolates. The gene junction regions showed more sequence variability, and in particular the intergenic regions showed a highest level of sequence variation. Although the clinical strains grew slower than the HRSVA2 virus isolate in tissue culture, the HRSVA2 isolate and clinical strains formed similar virus structures such as virus filaments and inclusion bodies in infected cells; supporting the clinical relevance of these virus structures. Conclusion This is the first report to describe the complete genetic characterization of HRSV clinical strains that have been sequenced directly from clinical

A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue

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Emanuele Rizzuto

2016-01-01

Full Text Available Tissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of generating contractile forces is dependent on the internal fiber organization and is reflected by some macroscopic parameters, such as the spontaneous contraction. Here we propose the application of digital image correlation (DIC as an independent tool for an accurate and noninvasive measurement of engineered muscle tissue spontaneous contraction. To validate the proposed technique we referred to the X-MET, a promising 3-dimensional model of skeletal muscle. The images acquired through a high speed camera were correlated with a custom-made algorithm and the longitudinal strain predictions were employed for measuring the spontaneous contraction. The spontaneous contraction reference values were obtained by studying the force response. The relative error between the spontaneous contraction frequencies computed in both ways was always lower than 0.15%. In conclusion, the use of a DIC based system allows for an accurate and noninvasive measurement of biological tissues’ spontaneous contraction, in addition to the measurement of tissue strain field on any desired region of interest during electrical stimulation.

Increasing Accuracy of Tissue Shear Modulus Reconstruction Using Ultrasonic Strain Tensor Measurement

Science.gov (United States)

Sumi, C.

Previously, we developed three displacement vector measurement methods, i.e., the multidimensional cross-spectrum phase gradient method (MCSPGM), the multidimensional autocorrelation method (MAM), and the multidimensional Doppler method (MDM). To increase the accuracies and stabilities of lateral and elevational displacement measurements, we also developed spatially variant, displacement component-dependent regularization. In particular, the regularization of only the lateral/elevational displacements is advantageous for the lateral unmodulated case. The demonstrated measurements of the displacement vector distributions in experiments using an inhomogeneous shear modulus agar phantom confirm that displacement-component-dependent regularization enables more stable shear modulus reconstruction. In this report, we also review our developed lateral modulation methods that use Parabolic functions, Hanning windows, and Gaussian functions in the apodization function and the optimized apodization function that realizes the designed point spread function (PSF). The modulations significantly increase the accuracy of the strain tensor measurement and shear modulus reconstruction (demonstrated using an agar phantom).

Strain-time cell death threshold for skeletal muscle in a tissue-engineered model system for deep tissue injury

NARCIS (Netherlands)

Gefen, A.; Nierop, van B.J.; Bader, D.L.; Oomens, C.W.J.

2008-01-01

Deep tissue injury (DTI) is a severe pressure ulcer that results from sustained deformation of muscle tissue overlying bony prominences. In order to understand the etiology of DTI, it is essential to determine the tolerance of muscle cells to large mechanical strains. In this study, a new

Design of triaxial test with controlled suction: measure of strain

International Nuclear Information System (INIS)

Gasc-Barbier, M.; Cosenza, Ph.; Ghoreychi, M.; Chanchole, S.; Cosenza, Ph.; Tessier, D.

2000-01-01

Experimental study of mechanical behavior of clayey materials under hygrometric condition is usually performed either on unloaded samples or by means of classical odometer tests used in soil mechanics. Such methods are not well adapted to hard deep clayey rocks with little deformability, porosity and permeability. Moreover, stress and strain tensors having a significant effect on hygro-mechanical behaviour and properties cannot be measured and investigated appropriately by classical tests. This is why a specific triaxial test was designed in which the sample is surrounded by a fiber glass tissue allowing air circulation and then by silicon on which confining pressure is applied. Thus, equilibrium between air and sample was reduced. Stress and strain tensors were also measured in time on the sample subjected to a mechanical loading and to a controlled suction. After presentation of the test, preliminary results are given. (authors)

Matrix production and organization by endothelial colony forming cells in mechanically strained engineered tissue constructs.

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Nicky de Jonge

Full Text Available AIMS: Tissue engineering is an innovative method to restore cardiovascular tissue function by implanting either an in vitro cultured tissue or a degradable, mechanically functional scaffold that gradually transforms into a living neo-tissue by recruiting tissue forming cells at the site of implantation. Circulating endothelial colony forming cells (ECFCs are capable of differentiating into endothelial cells as well as a mesenchymal ECM-producing phenotype, undergoing Endothelial-to-Mesenchymal-transition (EndoMT. We investigated the potential of ECFCs to produce and organize ECM under the influence of static and cyclic mechanical strain, as well as stimulation with transforming growth factor β1 (TGFβ1. METHODS AND RESULTS: A fibrin-based 3D tissue model was used to simulate neo-tissue formation. Extracellular matrix organization was monitored using confocal laser-scanning microscopy. ECFCs produced collagen and also elastin, but did not form an organized matrix, except when cultured with TGFβ1 under static strain. Here, collagen was aligned more parallel to the strain direction, similar to Human Vena Saphena Cell-seeded controls. Priming ECFC with TGFβ1 before exposing them to strain led to more homogenous matrix production. CONCLUSIONS: Biochemical and mechanical cues can induce extracellular matrix formation by ECFCs in tissue models that mimic early tissue formation. Our findings suggest that priming with bioactives may be required to optimize neo-tissue development with ECFCs and has important consequences for the timing of stimuli applied to scaffold designs for both in vitro and in situ cardiovascular tissue engineering. The results obtained with ECFCs differ from those obtained with other cell sources, such as vena saphena-derived myofibroblasts, underlining the need for experimental models like ours to test novel cell sources for cardiovascular tissue engineering.

Poisson's ratio of collagen fibrils measured by small angle X-ray scattering of strained bovine pericardium

Energy Technology Data Exchange (ETDEWEB)

Wells, Hannah C.; Sizeland, Katie H.; Kayed, Hanan R.; Haverkamp, Richard G., E-mail: r.haverkamp@massey.ac.nz [School of Engineering and Advanced Technology, Massey University, Private Bag 11222, Palmerston North 4442 (New Zealand); Kirby, Nigel; Hawley, Adrian; Mudie, Stephen T. [Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168 (Australia)

2015-01-28

Type I collagen is the main structural component of skin, tendons, and skin products, such as leather. Understanding the mechanical performance of collagen fibrils is important for understanding the mechanical performance of the tissues that they make up, while the mechanical properties of bulk tissue are well characterized, less is known about the mechanical behavior of individual collagen fibrils. In this study, bovine pericardium is subjected to strain while small angle X-ray scattering (SAXS) patterns are recorded using synchrotron radiation. The change in d-spacing, which is a measure of fibril extension, and the change in fibril diameter are determined from SAXS. The tissue is strained 0.25 (25%) with a corresponding strain in the collagen fibrils of 0.045 observed. The ratio of collagen fibril width contraction to length extension, or the Poisson's ratio, is 2.1 ± 0.7 for a tissue strain from 0 to 0.25. This Poisson's ratio indicates that the volume of individual collagen fibrils decreases with increasing strain, which is quite unlike most engineering materials. This high Poisson's ratio of individual fibrils may contribute to high Poisson's ratio observed for tissues, contributing to some of the remarkable properties of collagen-based materials.

Hamp1 mRNA and plasma hepcidin levels are influenced by sex and strain but do not predict tissue iron levels in inbred mice.

Science.gov (United States)

McLachlan, Stela; Page, Kathryn E; Lee, Seung-Min; Loguinov, Alex; Valore, Erika; Hui, Simon T; Jung, Grace; Zhou, Jie; Lusis, Aldons J; Fuqua, Brie; Ganz, Tomas; Nemeth, Elizabeta; Vulpe, Chris D

2017-11-01

Iron homeostasis is tightly regulated, and the peptide hormone hepcidin is considered to be a principal regulator of iron metabolism. Previous studies in a limited number of mouse strains found equivocal sex- and strain-dependent differences in mRNA and serum levels of hepcidin and reported conflicting data on the relationship between hepcidin ( Hamp1 ) mRNA levels and iron status. Our aim was to clarify the relationships between strain, sex, and hepcidin expression by examining multiple tissues and the effects of different dietary conditions in multiple inbred strains. Two studies were done: first, Hamp1 mRNA, liver iron, and plasma diferric transferrin levels were measured in 14 inbred strains on a control diet; and second, Hamp1 mRNA and plasma hepcidin levels in both sexes and iron levels in the heart, kidneys, liver, pancreas, and spleen in males were measured in nine inbred/recombinant inbred strains raised on an iron-sufficient or high-iron diet. Both sex and strain have a significant effect on both hepcidin mRNA (primarily a sex effect) and plasma hepcidin levels (primarily a strain effect). However, liver iron and diferric transferrin levels are not predictors of Hamp1 mRNA levels in mice fed iron-sufficient or high-iron diets, nor are the Hamp1 mRNA and plasma hepcidin levels good predictors of tissue iron levels, at least in males. We also measured plasma erythroferrone, performed RNA-sequencing analysis of liver samples from six inbred strains fed the iron-sufficient, low-iron, or high-iron diets, and explored differences in gene expression between the strains with the highest and lowest hepcidin levels. NEW & NOTEWORTHY Both sex and strain have a significant effect on both hepcidin mRNA (primarily a sex effect) and plasma hepcidin levels (primarily a strain effect). Liver iron and diferric transferrin levels are not predictors of Hamp1 mRNA levels in mice, nor are the Hamp1 mRNA and plasma hepcidin levels good predictors of tissue iron levels, at least

Prediction of Local Ultimate Strain and Toughness of Trabecular Bone Tissue by Raman Material Composition Analysis

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Roberto Carretta

2015-01-01

Full Text Available Clinical studies indicate that bone mineral density correlates with fracture risk at the population level but does not correlate with individual fracture risk well. Current research aims to better understand the failure mechanism of bone and to identify key determinants of bone quality, thus improving fracture risk prediction. To get a better understanding of bone strength, it is important to analyze tissue-level properties not influenced by macro- or microarchitectural factors. The aim of this pilot study was to identify whether and to what extent material properties are correlated with mechanical properties at the tissue level. The influence of macro- or microarchitectural factors was excluded by testing individual trabeculae. Previously reported data of mechanical parameters measured in single trabeculae under tension and bending and its compositional properties measured by Raman spectroscopy was evaluated. Linear and multivariate regressions show that bone matrix quality but not quantity was significantly and independently correlated with the tissue-level ultimate strain and postyield work (r=0.65–0.94. Principal component analysis extracted three independent components explaining 86% of the total variance, representing elastic, yield, and ultimate components according to the included mechanical parameters. Some matrix parameters were both included in the ultimate component, indicating that the variation in ultimate strain and postyield work could be largely explained by Raman-derived compositional parameters.

Three-dimensional development of tensile pre-strained annulus fibrosus cells for tissue regeneration: An in-vitro study

Energy Technology Data Exchange (ETDEWEB)

Chuah, Yon Jin [School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459 (Singapore); Lee, Wu Chean [University Hospital Conventry & Warwickshire NHS Trust, Clifford Bridge Road, West Midlands CV2, 2DX (United Kingdom); Wong, Hee Kit [Department of Orthopedic Surgery, National University Health System, NUHS Tower Block Level 11, 1E Kent Ridge Road, Singapore 119228 (Singapore); Kang, Yuejun, E-mail: yuejun.kang@ntu.edu.sg [School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459 (Singapore); Hee, Hwan Tak, E-mail: HTHee@ntu.edu.sg [School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459 (Singapore); Pinnacle Spine & Scoliosis Centre, 3 Mount Elizabeth, Mount Elizabeth Medical Centre, #04-07, Singapore 228510 (Singapore); School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637459 (Singapore)

2015-02-01

Prior research has investigated the immediate response after application of tensile strain on annulus fibrosus (AF) cells for the past decade. Although mechanical strain can produce either catabolic or anabolic consequences to the cell monolayer, little is known on how to translate these findings into further tissue engineering applications. Till to date, the application and effect of tensile pre-strained cells to construct a three-dimensional (3D) AF tissue remains unknown. This study aims to investigate the effect of tensile pre-strained exposure of 1 to 24 h on the development of AF pellet culture for 3 weeks. Equibiaxial cyclic tensile strain was applied on AF monolayer cells over a period of 24 h, which was subsequently developed into a cell pellet. Investigation on cellular proliferation, phenotypic gene expression, and histological changes revealed that tensile pre-strain for 24 h had significant and lasting effect on the AF tissue development, with enhanced cell proliferation, and up-regulation of collagen type I, II, and aggrecan expression. Our results demonstrated the regenerative ability of AF cell pellets subjected to 24 h tensile pre-straining. Knowledge on the effects of tensile pre-strain exposure is necessary to optimize AF development for tissue reconstruction. Moreover, the tensile pre-strained cells may further be utilized in either cell therapy to treat mild disc degeneration disease, or the development of a disc construct for total disc replacement. - Highlights: • Establishment of tensile pre-strained cell line population for annulus development. • Tensile strain limits collagen gene expression declination in monolayer culture. • Tensile pre-strained cells up-regulate their matrix protein in 3D pellet culture.

Value of the Strain Ratio on Ultrasonic Elastography for Differentiation of Benign and Malignant Soft Tissue Tumors.

Science.gov (United States)

Hahn, Seok; Lee, Young Han; Lee, Seung Hyun; Suh, Jin-Suck

2017-01-01

The purpose of this study was to evaluate whether the strain ratio provides additional value to conventional visual elasticity scores in the differentiation of benign and malignant soft tissue tumors by ultrasonic elastography. The Institutional Review Board approved the protocol of this retrospective review. Seventy-three patients who underwent elastography and had a soft tissue mass pathologically confirmed by ultrasound-guided core biopsy or surgical excision were enrolled from April 2012 through October 2014. On elastography, elasticity scores were determined with a 5-point visual scale, and the strain ratio to adjacent soft tissue at the same depth was calculated. Tumors were divided into benign and malignant groups according to the pathologic diagnoses. Elasticity scores and strain ratios were compared between benign and malignant groups, and diagnostic performance was evaluated by receiver operating characteristic curves. Of the 73 patients, 40 had benign tumors, and 33 had malignant tumors. Strain ratios (P = .003) and elasticity scores (P = .048) were significantly different between pathologic results. The areas under the receiver operating characteristic curves were 0.700 (95% confidence interval, 0.581-0.802) for the strain ratio and 0.623 (95% confidence interval, 0.515-0.746) for elastography. The strain ratios of malignant soft tissue tumors were lower than those of benign tumors and showed better diagnostic performance than did elasticity scores. The strain ratio can be used as a diagnostic indicator to predict the malignant potential of soft tissue tumors. © 2016 by the American Institute of Ultrasound in Medicine.

Whole body and tissue blood volumes of two strains of rainbow trout (Oncorhynchus mykiss)

Science.gov (United States)

Gingerich, W.H.; Pityer, R.A.; Rach, J.J.

1990-01-01

1. Estimates of apparent packed cell, plasma and total blood volumes for the whole body and for 13 selected tissues were compared between Kamloops and Wytheville strains of rainbow trout (Oncorhynchus mykiss) by the simultaneous injection of two vascular tracers, radiolabeled trout erythrocytes (51Cr-RBC) and radioiodated bovine serum albumin (125I-BSA).2. Whole body total blood volume, plasma volume and packed cell volume were slightly, but not significantly greater in the Wytheville trout, whereas, the apparent plasma volumes and total blood volumes in 4 of 13 tissues were significantly greater in the Kamloops strain.3. Differences were most pronounced in highly perfused organs, such as the liver and kidney and in organs of digestion such as the stomach and intestines.4. Differences in blood volumes between the two strains may be related to the greater permeability of the vascular membranes in the Kamloops strain fish.

Soft Tissue Strain Rates in Side-Blast Incidents

Science.gov (United States)

2014-11-02

increase of strain rate is known to cause the stiffening of soft connective tissues ( Haut and Haut 1997 [49]; Panjabi et al. 1998 [50]; Crisco et al...Réseau Québécois de Calcul de Haute Performance, with a peak compute performance of 27 596 GFlops). Figure 2: Torso motion imposed in the model...Yan YP. 2003. Mechanical properties of nasal fascia and periosteum. Clinical Biomechanics. 18:760-764. [49] Haut TL, Haut RC. 1997. The state of

Methodology to measure strains at high temperatures using electrical strain gages with free filaments

International Nuclear Information System (INIS)

Atanazio Filho, Nelson N.; Gomes, Paulo T. Vida; Scaldaferri, Denis H.B.; Silva, Luiz L. da; Rabello, Emerson G.; Mansur, Tanius R.

2013-01-01

An experimental methodology used for strains measuring at high temperatures is show in this work. In order to do the measurements, it was used electric strain gages with loose filaments attached to a stainless steel 304 beam with specific cements. The beam has triangular shape and a constant thickness, so the strain is the same along its length. Unless the beam surface be carefully prepared, the strain gage attachment is not efficient. The showed results are for temperatures ranging from 20 deg C to 300 deg C, but the experimental methodology could be used to measure strains at a temperature up to 900 deg C. Analytical calculations based on solid mechanics were used to verify the strain gage electrical installation and the measured strains. At a first moment, beam deformations as a temperature function were plotted. After that, beam deformations with different weighs were plotted as a temperature function. The results shown allowed concluding that the experimental methodology is trustable to measure strains at temperatures up to 300 deg C. (author)

Instrument for measuring fuel cladding strain

International Nuclear Information System (INIS)

Billeter, T.R.

1976-01-01

Development work to provide instrumentation for the continuous measurement of strain of material specimens such as nuclear fuel cladding has shown that a microwave sensor and associated instrumentation hold promise. The cylindrical sensor body enclosing the specimen results in a coaxial resonator absorbing microwave energy at frequencies dependent upon the diameter of the specimen. Diametral changes of a microinch can be resolved with use of the instrumentation. Very reasonable values of elastic strain were measured at 75 0 F and 1000 0 F for an internally pressurized 20 percent C.W. 316 stainless steel specimen simulating nuclear fuel cladding. The instrument also indicated the creep strain of the same specimen pressurized at 6500 psi and at a temperature of 1000 0 F for a period of 700 hours. Although the indicated strain appears greater than actual, the sensor/specimen unit experienced considerable oxidation even though an inert gas purge persisted throughout the test duration. By monitoring at least two modes of resonance, the measured strain was shown to be nearly independent of sensor temperature. To prevent oxidation, a second test was performed in which the specimen/sensor units were contained in an evacuated enclosure. The strain of the two prepressurized specimens as indicated by the microwave instrumentation agreed very closely with pre- and post-test measurements obtained with use of a laser interferometer

Experimental Toxoplasmosis in Rats Induced Orally with Eleven Strains of Toxoplasma gondii of Seven Genotypes: Tissue Tropism, Tissue Cyst Size, Neural Lesions, Tissue Cyst Rupture without Reactivation, and Ocular Lesions.

Directory of Open Access Journals (Sweden)

Jitender P Dubey

Full Text Available The protozoan parasite Toxoplasma gondii is one of the most widely distributed and successful parasites. Toxoplasma gondii alters rodent behavior such that infected rodents reverse their fear of cat odor, and indeed are attracted rather than repelled by feline urine. The location of the parasite encysted in the brain may influence this behavior. However, most studies are based on the highly susceptible rodent, the mouse.Latent toxoplasmosis was induced in rats (10 rats per T. gondii strains of the same age, strain, and sex, after oral inoculation with oocysts (natural route and natural stage of infection of 11 T. gondii strains of seven genotypes. Rats were euthanized at two months post inoculation (p.i. to investigate whether the parasite genotype affects the distribution, location, tissue cyst size, or lesions. Tissue cysts were enumerated in different regions of the brains, both in histological sections as well in saline homogenates. Tissue cysts were found in all regions of the brain. The tissue cyst density in different brain regions varied extensively between rats with many regions highly infected in some animals. Overall, the colliculus was most highly infected although there was a large amount of variability. The cerebral cortex, thalamus, and cerebellum had higher tissue cyst densities and two strains exhibited tropism for the colliculus and olfactory bulb. Histologically, lesions were confined to the brain and eyes. Tissue cyst rupture was frequent with no clear evidence for reactivation of tachyzoites. Ocular lesions were found in 23 (25% of 92 rat eyes at two months p.i. The predominant lesion was focal inflammation in the retina. Tissue cysts were seen in the sclera of one and in the optic nerve of two rats. The choroid was not affected. Only tissue cysts, not active tachyzoite infections, were detected. Tissue cysts were seen in histological sections of tongue of 20 rats but not in myocardium and leg muscle.This study reevaluated

Strain measurement on a compact nuclear reactor steam generator

International Nuclear Information System (INIS)

Scaldaferri, Denis Henrique Bianchi; Gomes, Paulo de Tarso Vida; Mansur, Tanius Rodrigues; Pozzo, Renato del; Mola, Jairo

2011-01-01

This work presents the strain measurement procedures applied to a compact nuclear reactor steam generator, during a hydrostatic test, using strain gage technology. The test was divided in two steps: primary side test and secondary side test. In the primary side test twelve points for strain measurement using rectangular rosettes, three points (two external and one internal) for temperature measurement using special strain gages and one point for pressure measurement using a pressure transducer were monitored. In the secondary side test 18 points for strain measurement using rectangular rosettes, four points (two external and two internal) for temperature measurement using special strain gages and one point for pressure measurement using a pressure transducer were monitored. The measurement points on both internal and external pressurizer walls were established from pre-calculated stress distribution by means of numerical approach (finite elements modeling). Strain values using a quarter Wheatstone bridge circuit were obtained. Stress values, from experimental strain were determined, and to numerical calculation results were compared. (author)

Thermal strain measurement of EAST W/Cu divertor structure using electric resistance strain gauges

Energy Technology Data Exchange (ETDEWEB)

Wang, Xingli [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Wang, Wanjing, E-mail: wjwang@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Wang, Jichao [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Wei, Ran; Sun, Zhaoxuan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Li, Qiang; Xie, Chunyi [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Chen, Hong-En; Wang, Kaiqiang; Wu, Lei; Chen, Zhenmao [State Key Lab for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University (China); Luo, Guang-Nan [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031 (China); Science Island Branch of Graduate School, University of Science & Technology of China, Hefei, 230031 (China); Hefei Center for Physical Science and Technology, Hefei, 230022 (China); Hefei Science Center of Chinese Academy of Sciences, Hefei, 230027 (China)

2016-12-15

Highlights: • To understand the service behavior of W/Cu divertor, an electrical resistance strain gauge system had been introduced in a thermal strain measurement experiment. • The measurement system successfully finished the experiment and obtained valued thermal strain data. • Two thermomechanical analyses had also been carried out and compared with the measurement results. • Experiment results corresponded well to simulations and threw a light upon the failure of W/Cu divertor in the previous baking tests. - Abstract: W/Cu divertor has complex structure and faces extreme work environment in EAST Tokamak device. To measure its thermal strain shall be a valued way to understand its service behavior and then optimize its design and manufacturing process. This work presents a preliminary study on measuring thermal strain of EAST W/Cu divertor structure using electric resistance strain gauges. Eight gauges had been used in the experiment and the heating temperature had been set to 230 °C with respect to the work temperature. To realize the measuring experiment, an appropriate fixing method of gauges in divertor narrow spaces had been taken and tested, which could not only withstand high temperature but also had no damage to the divertor sample. The measurement results were that three gauges showed positive strain while other three showed negative strain after having been compensated, which corresponded to tensile stress and compressed stress respectively. Two thermomechanical simulations had also been carried out and used for comparing with the experiment.

Apt strain measurement technique for impulsive loading applications

International Nuclear Information System (INIS)

Nanda, Soumya Ranjan; Kulkarni, Vinayak; Sahoo, Niranjan

2017-01-01

The necessity of precise measurement of strain time history for impulsive loading applications has been addressed in the present investigation. Finite element modeling is initially carried out for a hemispherical test model and stress bar assembly to arrive at an appropriate location for strain measurement. In dynamic calibration experiments, strain measurements are performed using two wire and three wire quarter bride arrangements along with half bridge circuit. Usefulness of these arrangements has been verified by analyzing strain signals in time and frequency domains. Comparison of recovered force time histories proved that the half bridge circuit is the most suitable for such applications. Actual shock tube testing of the instrumented hemispherical test model confirmed the applicability of half bridge circuit for short duration strain measurements. (technical note)

Strain localization band width evolution by electronic speckle pattern interferometry strain rate measurement

Energy Technology Data Exchange (ETDEWEB)

Guelorget, Bruno [Institut Charles Delaunay-LASMIS, Universite de technologie de Troyes, FRE CNRS 2848, 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France)], E-mail: bruno.guelorget@utt.fr; Francois, Manuel; Montay, Guillaume [Institut Charles Delaunay-LASMIS, Universite de technologie de Troyes, FRE CNRS 2848, 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France)

2009-04-15

In this paper, electronic speckle pattern interferometry strain rate measurements are used to quantify the width of the strain localization band, which occurs when a sheet specimen is submitted to tension. It is shown that the width of this band decreases with increasing strain. Just before fracture, this measured width is about five times wider than the shear band and the initial sheet thickness.

Invasion of Porphyromonas gingivalis strains into vascular cells and tissue

Directory of Open Access Journals (Sweden)

Ingar Olsen

2015-08-01

Full Text Available Porphyromonas gingivalis is considered a major pathogen in adult periodontitis and is also associated with multiple systemic diseases, for example, cardiovascular diseases. One of its most important virulence factors is invasion of host cells. The invasion process includes attachment, entry/internalization, trafficking, persistence, and exit. The present review discusses these processes related to P. gingivalis in cardiovascular cells and tissue. Although most P. gingivalis strains invade, the invasion capacity of strains and the mechanisms of invasion including intracellular trafficking among them differ. This is consistent with the fact that there are significant differences in the pathogenicity of P. gingivalis strains. P. gingivalis invasion mechanisms are also dependent on types of host cells. Although much is known about the invasion process of P. gingivalis, we still have little knowledge of its exit mechanisms. Nevertheless, it is intriguing that P. gingivalis can remain viable in human cardiovascular cells and atherosclerotic plaque and later exit and re-enter previously uninfected host cells.

Stress-sensitive tissue regeneration in viscoelastic biomaterials subjected to modulated tensile strain.

Science.gov (United States)

Belfiore, Laurence A; Floren, Michael L; Paulino, Alexandre T; Belfiore, Carol J

2011-09-01

This research contribution addresses the mechanochemistry of intra-tissue mass transfer for nutrients, oxygen, growth factors, and other essential ingredients that anchorage-dependent cells require for successful proliferation on biocompatible surfaces. The unsteady state reaction-diffusion equation (i.e., modified diffusion equation) is solved according to the von Kármán-Pohlhausen integral method of boundary layer analysis when nutrient consumption and tissue regeneration are stimulated by harmonically imposed stress. The mass balance with diffusion and stress-sensitive kinetics represents a rare example where the Damköhler and Deborah numbers appear together in an effort to simulate the development of mass transfer boundary layers in porous viscoelastic biomaterials. The Boltzmann superposition integral is employed to calculate time-dependent strain in terms of the real and imaginary components of dynamic compliance for viscoelastic solids that transmit harmonic excitation to anchorage-dependent cells. Rates of nutrient consumption under stress-free conditions are described by third-order kinetics which include local mass densities of nutrients, oxygen, and attached cells that maintain dynamic equilibrium with active protein sites in the porous matrix. Thinner nutrient mass transfer boundary layers are stabilized at shorter dimensionless diffusion times when the stress-free intra-tissue Damköhler number increases above its initial-condition-sensitive critical value. The critical stress-sensitive intra-tissue Damköhler number, above which it is necessary to consider the effect of harmonic strain on nutrient consumption and tissue regeneration, is proportional to the Deborah number and corresponds to a larger fraction of the stress-free intra-tissue Damköhler number in rigid biomaterials. Copyright © 2011 Elsevier B.V. All rights reserved.

Strain gauge measurement uncertainties on hydraulic turbine runner blade

International Nuclear Information System (INIS)

Arpin-Pont, J; Gagnon, M; Tahan, S A; Coutu, A; Thibault, D

2012-01-01

Strains experimentally measured with strain gauges can differ from those evaluated using the Finite Element (FE) method. This difference is due mainly to the assumptions and uncertainties inherent to each method. To circumvent this difficulty, we developed a numerical method based on Monte Carlo simulations to evaluate measurement uncertainties produced by the behaviour of a unidirectional welded gauge, its position uncertainty and its integration effect. This numerical method uses the displacement fields of the studied part evaluated by an FE analysis. The paper presents a study case using in situ data measured on a hydraulic turbine runner. The FE analysis of the turbine runner blade was computed, and our numerical method used to evaluate uncertainties on strains measured at five locations with welded strain gauges. Then, measured strains and their uncertainty ranges are compared to the estimated strains. The uncertainty ranges obtained extended from 74 με to 165 με. Furthermore, the biases observed between the median of the uncertainty ranges and the FE strains varied from −36 to 36 με. Note that strain gauge measurement uncertainties depend mainly on displacement fields and gauge geometry.

Measuring autogenous strain of concrete with corrugated moulds

DEFF Research Database (Denmark)

Tian, Qian; Jensen, Ole Mejlhede

2008-01-01

A reliable technique to quantify autogenous strain is a prerequisite to numerical modeling in stress calculations for high performance concrete. The introducing of a special kind of corrugated tube mould helps to transforming volume strain measurement into liner strain measurement in horizontal...... direction for fluid concrete, which not only realizes the continuous monitoring of the autogenous shrinkage since casting, but also effectively eliminates the disturbance resulting from gravity, temperature variation and mould restraint on measuring results. Based on this measuring technique, this paper...

Increased cytotoxicity and streptolysin O activity in group G streptococcal strains causing invasive tissue infections

DEFF Research Database (Denmark)

Siemens, Nikolai; Kittang, Bård R; Chakrakodi, Bhavya

2015-01-01

Streptococcus dysgalactiae subsp. equisimilis (SDSE) has emerged as an important cause of severe skin and soft tissue infections, but little is known of the pathogenic mechanisms underlying tissue pathology. Patient samples and a collection of invasive and non-invasive group G SDSE strains (n = 6...

Temperature measurement by thermal strain imaging with diagnostic power ultrasound, with potential for thermal index determination.

Science.gov (United States)

Liang, Hai-Dong; Zhou, Li-Xia; Wells, Peter N T; Halliwell, Michael

2009-05-01

Over the years, there has been a substantial increase in acoustic exposure in diagnostic ultrasound as new imaging modalities with higher intensities and frame rates have been introduced; and more electronic components have been packed into the probe head, so that there is a tendency for it to become hotter. With respect to potential thermal effects, including those which may be hazardous occurring during ultrasound scanning, there is a correspondingly growing need for in vivo techniques to guide the operator as to the actual temperature rise occurring in the examined tissues. Therefore, an in vivo temperature estimator would be of considerable practical value. The commonly-used method of tissue thermal index (TI) measurement with a hydrophone in water could underestimate the actual value of TI (in one report by as much as 2.9 times). To obtain meaningful results, it is necessary to map the temperature elevation in 2-D (or 3-D) space. We present methodology, results and validation of a 2-D spatial and temporal thermal strain ultrasound temperature estimation technique in phantoms, and its apparently novel application in tracking the evolution of heat deposition at diagnostic exposure levels. The same ultrasound probe is used for both transmission and reception. The displacement and thermal strain estimation methods are similar to those used in high-intensity focused ultrasound thermal monitoring. The use of radiofrequency signals permits the application of cross correlation as a similarity measurement for tracking feature displacement. The displacement is used to calculate the thermal strain directly related to the temperature rise. Good agreement was observed between the temperature rise and the ultrasound power and scan duration. Thermal strain up to 1.4% was observed during 4000-s scan. Based on the results obtained for the temperature range studied in this work, the technique demonstrates potential for applicability in phantom (and possibly in vivo tissue

Standard guide for high-temperature static strain measurement

CERN Document Server

American Society for Testing and Materials. Philadelphia

1998-01-01

1.1 This guide covers the selection and application of strain gages for the measurement of static strain up to and including the temperature range from 425 to 650°C (800 to 1200°F). This guide reflects some current state-of-the-art techniques in high temperature strain measurement, and will be expanded and updated as new technology develops. 1.2 This practice assumes that the user is familiar with the use of bonded strain gages and associated signal conditioning and instrumentation as discussed in Refs. (1) and (2). The strain measuring systems described are those that have proven effective in the temperature range of interest and were available at the time of issue of this practice. It is not the intent of this practice to limit the user to one of the gage types described nor is it the intent to specify the type of system to be used for a specific application. However, in using any strain measuring system including those described, the proposer must be able to demonstrate the capability of the proposed sy...

Engineering strain measurements using the NPD at LANSCE

International Nuclear Information System (INIS)

Bourke, M.A.M.; Goldstone, J.A.; Lovell, K.J.

1991-01-01

The presence of residual stress in engineering components can affect their mechanical properties and structural integrity. Neutron diffraction is the only measuring technique which can provide spatially resolved non-destructive strain measurements in the interior of a component. By recording the change in the interplanar spacings elastic strains can be measured for individual lattice reflections. Also on a pulsed source, where all lattice reflections are recorded, profile refinement is an option which allows the strain to be obtained from changes in the lattice parameter. Measurements made at LANSCE demonstrate the potential for stress measurements on a pulsed source and indicate the advantages and disadvantages over measurements made on a reactor. (author)

Correlation between the tissue Doppler, strain rate, strain imaging during the dobutamine infusion and coronary fractional flow reserve during catheterization: a comparative study.

Science.gov (United States)

Dagdelen, Sinan; Yuce, Murat; Emiroglu, Yunus; Ergelen, Mehmet; Pala, Selcuk; Tanalp, Ali Cevat; Izgi, Akin; Kirma, Cevat

2005-06-22

Coronary fractional flow reserve (FFR) as an invasive, and dobutamine stress echocardiography (DSE) as a noninvasive technique were used to detect critical coronary stenosis. This study was undertaken to assess correlation between these two techniques by using tissue Doppler, strain rate (SR), and strain imaging (S). In 17 patients (aged 54.9+/-12.6, 4 F), a total of 22 vessels were studied. On dobutamine stress echocardiography, baseline and peak systolic (Sm), early (Em) and late (Am) diastolic myocardial velocities, SR and S were recorded from parasternal view (mid-posterior segment) for radial and apical view (mid-septum) for longitudinal deformation. Then coronary FFR was performed by using intracoronary adenosine infusion, and the value of system were analyzed for longitudinal SR and S values, it had a mild correlation with SR (r = 0.47, p = 0.044) and a good correlation with S (r = 0.66, p = 0.002). The quantification of regional myocardial deformation by using DSE rather than the motion would be more appropriate in detecting the ischemic dysfunctional segment supplied by the critical coronary stenosis. Strain measurement during the dobutamine infusion may provide an information on the FFR results of the culprit vessel.

Quantitation of stress echocardiography by tissue Doppler and strain rate imaging: a dream come true?

Science.gov (United States)

Galderisi, Maurizio; Mele, Donato; Marino, Paolo Nicola

2005-01-01

Tissue Doppler (TD) is an ultrasound tool providing a quantitative agreement of left ventricular regional myocardial function in different modalities. Spectral pulsed wave (PW) TD, performed online during the examination, measures instantaneous myocardial velocities. By means of color TD, velocity images are digitally stored for subsequent off-line analysis and mean myocardial velocities are measured. An implementation of color TD includes strain rate imaging (SRI), based on post-processing conversion of regional velocities in local myocardial deformation rate (strain rate) and percent deformation (strain). These three modalities have been applied to stress echocardiography for quantitative evaluation of regional left ventricular function and detection of ischemia and viability. They present advantages and limitations. PWTD does not permit the simultaneous assessment of multiple walls and therefore is not compatible with clinical stress echocardiography while it could be used in a laboratory setting. Color TD provides a spatial map of velocity throughout the myocardium but its results are strongly affected by the frame rate. Both color TD and PWTD are also influenced by overall cardiac motion and tethering from adjacent segments and require reference velocity values for interpretation of regional left ventricular function. High frame rate (i.e. > 150 ms) post-processing-derived SRI can potentially overcome these limitations, since measurements of myocardial deformation have not any significant apex-to-base gradient. Preliminary studies have shown encouraging results about the ability of SRI to detect ischemia and viability, in terms of both strain rate changes and/or evidence of post-systolic thickening. SRI is, however, Doppler-dependent and time-consuming. Further technical refinements are needed to improve its application and introduce new ultrasound modalities to overcome the limitations of the Doppler-derived deformation analysis.

A tissue adaptation model based on strain-dependent collagen degradation and contact-guided cell traction.

Science.gov (United States)

Heck, T A M; Wilson, W; Foolen, J; Cilingir, A C; Ito, K; van Donkelaar, C C

2015-03-18

Soft biological tissues adapt their collagen network to the mechanical environment. Collagen remodeling and cell traction are both involved in this process. The present study presents a collagen adaptation model which includes strain-dependent collagen degradation and contact-guided cell traction. Cell traction is determined by the prevailing collagen structure and is assumed to strive for tensional homeostasis. In addition, collagen is assumed to mechanically fail if it is over-strained. Care is taken to use principally measurable and physiologically meaningful relationships. This model is implemented in a fibril-reinforced biphasic finite element model for soft hydrated tissues. The versatility and limitations of the model are demonstrated by corroborating the predicted transient and equilibrium collagen adaptation under distinct mechanical constraints against experimental observations from the literature. These experiments include overloading of pericardium explants until failure, static uniaxial and biaxial loading of cell-seeded gels in vitro and shortening of periosteum explants. In addition, remodeling under hypothetical conditions is explored to demonstrate how collagen might adapt to small differences in constraints. Typical aspects of all essentially different experimental conditions are captured quantitatively or qualitatively. Differences between predictions and experiments as well as new insights that emerge from the present simulations are discussed. This model is anticipated to evolve into a mechanistic description of collagen adaptation, which may assist in developing load-regimes for functional tissue engineered constructs, or may be employed to improve our understanding of the mechanisms behind physiological and pathological collagen remodeling. Copyright © 2014 Elsevier Ltd. All rights reserved.

Strain-specific helper T cell profile in the gut-associated lymphoid tissue.

Science.gov (United States)

Stanisavljević, Suzana; Đedović, Neda; Vujičić, Milica; Saksida, Tamara; Jevtić, Bojan; Milovanović, Boško; Momčilović, Miljana; Miljković, Đorđe; Stojanović, Ivana

2017-10-01

C57BL/6, BALB/c and NOD mice are among the most frequently used strains in autoimmunity research. NOD mice spontaneously develop type 1 diabetes (T1D) and they are prone to induction of experimental autoimmune encephalomyelitis (EAE). Both diseases can be routinely induced in C57BL/6 mice, but not in BALB/c mice. Also, C57BL/6 mice are generally considered T helper (Th)1-biased and BALB/c Th2-biased mice. Having in mind increasingly appreciated role of gut associated lymphoid tissue (GALT) cells in autoimmunity, especially in relation to gut Th17 and regulatory T (Treg) cells, our aim was to determine if there are differences in proportion of CD4 + T cell populations in mesenteric lymph nodes and Peyer's patches of these mouse strains. Lower proportion of Treg was observed in NOD PP, Th2 cells dominated in BALB/c mice in mesenteric lymph nodes (MLN) and Peyer's patches (PP), while Th1 cells prevailed in C57BL/6 MLN. Intradermal immunization of mice with complete Freund's adjuvant resulted in significant difference in Th cell distribution in GALT of NOD mice. Differences were less pronounced in C57BL/6 mice, while GALT of BALB/c mice was almost unresponsive to the immunization. The observed strain- and tissue-dependent changes in Treg proportion after the immunization was probably a consequence of different CCR2 or CCR6-related migration patterns and/or in situ Treg proliferation. In conclusion, NOD, a highly autoimmunity-prone mouse strain, exhibits more profound GALT-related immune response upon immunization compared to the strains that are less prone to autoimmunity. Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

Comparison of contrast in brightness mode and strain ultrasonography of glial brain tumours

International Nuclear Information System (INIS)

Selbekk, Tormod; Brekken, Reidar; Indergaard, Marit; Solheim, Ole; Unsgård, Geirmund

2012-01-01

Image contrast between normal tissue and brain tumours may sometimes appear to be low in intraoperative ultrasound. Ultrasound imaging of strain is an image modality that has been recently explored for intraoperative imaging of the brain. This study aims to investigate differences in image contrast between ultrasound brightness mode (B-mode) images and ultrasound strain magnitude images of brain tumours. Ultrasound radiofrequency (RF) data was acquired during surgery in 15 patients with glial tumours. The data were subsequently processed to provide strain magnitude images. The contrast in the B-mode images and the strain images was determined in assumed normal brain tissue and tumour tissue at selected regions of interest (ROI). Three measurements of contrast were done in the ultrasound data for each patient. The B-mode and strain contrasts measurements were compared using the paired samples t- test. The statistical analysis of a total of 45 measurements shows that the contrasts in the strain magnitude images are significantly higher than in the conventional ultrasound B-mode images (P < 0.0001). The results indicate that ultrasound strain imaging provides better discrimination between normal brain tissue and glial tumour tissue than conventional ultrasound B-mode imaging. Ultrasound imaging of tissue strain therefore holds the potential of becoming a valuable adjunct to conventional intraoperative ultrasound imaging in brain tumour surgery

Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain

Directory of Open Access Journals (Sweden)

Vranckx Katleen

2012-03-01

Full Text Available Abstract Background Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia and is responsible for significant economic losses to the pig industry. To better understand the mode of action of a commercial, adjuvanted, inactivated whole cell vaccine and the influence of diversity on the efficacy of vaccination, we investigated samples from vaccinated and non-vaccinated pigs experimentally infected with either a low (LV or a highly virulent (HV M. hyopneumoniae strain. Non-vaccinated and sham-infected control groups were included. Lung tissue samples collected at 4 and 8 weeks post infection (PI were immunohistochemically tested for the presence of T-lymphocytes, B-lymphocytes and macrophages in the bronchus-associated lymphoid tissue (BALT. The number of M. hyopneumoniae organisms in bronchoalveolar lavage (BAL fluid was determined using quantitative PCR at 4 and 8 weeks PI. Serum antibodies against M. hyopneumoniae were determined at 0, 2, 4, 6 and 8 weeks PI. Results The immunostaining revealed a lower density of macrophages in the BALT of the vaccinated groups compared to the non-vaccinated groups. The highest number of M. hyopneumoniae organisms in the BAL fluid was measured at 4 weeks PI for the HV strain and at 8 weeks PI for the LV strain. Vaccination reduced the number of organisms non-significantly, though for the HV strain the reduction was clinically more relevant than for the LV strain. At the level of the individual pigs, a higher lung lesion score was associated with more M. hyopneumoniae organisms in the lungs and a higher density of the investigated immune cells in the BALT. Conclusions In conclusion, the infiltration of macrophages after infection with M. hyopneumoniae is reduced by vaccination. The M. hyopneumoniae replication in the lungs is also reduced in vaccinated pigs, though the HV strain is inhibited more than the LV strain.

Engineering strain measurements using the NPD at LANSCE

International Nuclear Information System (INIS)

Bourke, M.A.M.; Goldstone, J.A.; Lovell, K.J.

1990-01-01

The presence of residual stress in engineering components can affect their mechanical properties and structural integrity. Neutron diffraction is the only measuring technique which can provide spatially resolved non-destructive strain measurements in the interior of a component. By recording the change in the interplanar spacings elastic strains can be measured for individual lattice reflections. Also on a pulsed source, where all lattice reflections are recorded, profile refinement is an option which alloys the strain to be obtained from changes in the lattice parameter. Measurements made at LANSCE demonstrate the potential for stress measurements on a pulsed source and indicate the advantages and disadvantages over measurements made on a reactor. 5 refs., 5 figs

Dark field electron holography for strain measurement

Energy Technology Data Exchange (ETDEWEB)

Beche, A., E-mail: armand.beche@fei.com [CEA-Grenoble, INAC/SP2M/LEMMA, F-38054 Grenoble (France); Rouviere, J.L. [CEA-Grenoble, INAC/SP2M/LEMMA, F-38054 Grenoble (France); Barnes, J.P.; Cooper, D. [CEA-LETI, Minatec Campus, F-38054 Grenoble (France)

2011-02-15

Dark field electron holography is a new TEM-based technique for measuring strain with nanometer scale resolution. Here we present the procedure to align a transmission electron microscope and obtain dark field holograms as well as the theoretical background necessary to reconstruct strain maps from holograms. A series of experimental parameters such as biprism voltage, sample thickness, exposure time, tilt angle and choice of diffracted beam are then investigated on a silicon-germanium layer epitaxially embedded in a silicon matrix in order to obtain optimal dark field holograms over a large field of view with good spatial resolution and strain sensitivity. -- Research Highlights: {yields} Step by step explanation of the dark field electron holography technique. {yields} Presentation of the theoretical equations to obtain quantitative strain map. {yields} Description of experimental parameters influencing dark field holography results. {yields} Quantitative strain measurement on a SiGe layer embedded in a silicon matrix.

Strain rate measurement by Electronic Speckle Pattern Interferometry: A new look at the strain localization onset

International Nuclear Information System (INIS)

Guelorget, Bruno; Francois, Manuel; Vial-Edwards, Cristian; Montay, Guillaume; Daniel, Laurent; Lu, Jian

2006-01-01

In-plane Electronic Speckle Pattern Interferometry has been successfully used during tensile testing of semi-hard copper sheets in order to measure the strain rate. On one hand, heterogeneity in strain rate field has been found before the maximum of the tensile force (ε t ≅ 19.4 and 25.4%, respectively). Thus, a localization phenomenon occurs before the classic Considere's criterion (dF = 0) for the diffuse neck initiation. On the other hand, strain rate measurement before fracture shows the moment where one of the two slip band systems becomes predominant, then strain concentrates in a small area, the shear band. Uncertainty evaluation has been carried out, which shows a very good accuracy of the total strain and the strain rate measurements

Strain rate measurement by Electronic Speckle Pattern Interferometry: A new look at the strain localization onset

Energy Technology Data Exchange (ETDEWEB)

Guelorget, Bruno [Universite de Technologie de Troyes (UTT), Laboratoire des Systemes Mecaniques et d' ingenierie Simultanee (LASMIS, CNRS FRE 2719), 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France)]. E-mail: bruno.guelorget@utt.fr; Francois, Manuel [Universite de Technologie de Troyes (UTT), Laboratoire des Systemes Mecaniques et d' ingenierie Simultanee (LASMIS, CNRS FRE 2719), 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France); Vial-Edwards, Cristian [Departemento de Ingenieria Mecanica y Metalurgica, Pontificia Universidad Catolica de Chile, Vicuna Mackenna 4860, 6904411 Santiago (Chile); Montay, Guillaume [Universite de Technologie de Troyes (UTT), Laboratoire des Systemes Mecaniques et d' ingenierie Simultanee (LASMIS, CNRS FRE 2719), 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France); Daniel, Laurent [Universite de Technologie de Troyes (UTT), Laboratoire des Systemes Mecaniques et d' ingenierie Simultanee (LASMIS, CNRS FRE 2719), 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France); Lu, Jian [Universite de Technologie de Troyes (UTT), Laboratoire des Systemes Mecaniques et d' ingenierie Simultanee (LASMIS, CNRS FRE 2719), 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex (France)

2006-01-15

In-plane Electronic Speckle Pattern Interferometry has been successfully used during tensile testing of semi-hard copper sheets in order to measure the strain rate. On one hand, heterogeneity in strain rate field has been found before the maximum of the tensile force ({epsilon} {sup t} {approx_equal} 19.4 and 25.4%, respectively). Thus, a localization phenomenon occurs before the classic Considere's criterion (dF = 0) for the diffuse neck initiation. On the other hand, strain rate measurement before fracture shows the moment where one of the two slip band systems becomes predominant, then strain concentrates in a small area, the shear band. Uncertainty evaluation has been carried out, which shows a very good accuracy of the total strain and the strain rate measurements.

Internal displacement and strain measurement using digital volume correlation: a least-squares framework

International Nuclear Information System (INIS)

Pan, Bing; Wu, Dafang; Wang, Zhaoyang

2012-01-01

As a novel tool for quantitative 3D internal deformation measurement throughout the interior of a material or tissue, digital volume correlation (DVC) has increasingly gained attention and application in the fields of experimental mechanics, material research and biomedical engineering. However, the practical implementation of DVC involves important challenges such as implementation complexity, calculation accuracy and computational efficiency. In this paper, a least-squares framework is presented for 3D internal displacement and strain field measurement using DVC. The proposed DVC combines a practical linear-intensity-change model with an easy-to-implement iterative least-squares (ILS) algorithm to retrieve 3D internal displacement vector field with sub-voxel accuracy. Because the linear-intensity-change model is capable of accounting for both the possible intensity changes and the relative geometric transform of the target subvolume, the presented DVC thus provides the highest sub-voxel registration accuracy and widest applicability. Furthermore, as the ILS algorithm uses only first-order spatial derivatives of the deformed volumetric image, the developed DVC thus significantly reduces computational complexity. To further extract 3D strain distributions from the 3D discrete displacement vectors obtained by the ILS algorithm, the presented DVC employs a pointwise least-squares algorithm to estimate the strain components for each measurement point. Computer-simulated volume images with controlled displacements are employed to investigate the performance of the proposed DVC method in terms of mean bias error and standard deviation error. Results reveal that the present technique is capable of providing accurate measurements in an easy-to-implement manner, and can be applied to practical 3D internal displacement and strain calculation. (paper)

A piezoelectric transducer for measurement of dynamic strain in pipes

Energy Technology Data Exchange (ETDEWEB)

Lannes, Daniel P.; Gama, Antonio L. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Engenharia Mecanica

2009-07-01

This work presents a new strain transducer developed mainly for the inspection and evaluation of piping systems with excessive vibration. Vibration is one of the most common causes of piping failures. These failures could be avoided if the vibration problems were identified and quickly evaluated. Procedures for evaluation of piping vibration are usually based on pipe velocity or displacement. Although simple and fast, these procedures do not provide precise information on the risk of piping fatigue failure. Through the measurement of pipe dynamic strains the risk of failure due to vibration can be determined more accurately. The measurement of strain is usually performed using the conventional strain gauge method. Although efficient and accurate, the implementation of the conventional strain gauge technique may become a difficult task in certain industrial scenarios. Motivated by the need of a simple and rapid method for pipe dynamic strain measurement, a piezoelectric dynamic strain transducer was developed. This work presents a description of the piezoelectric strain transducer and the preliminary results of pipe strain measurements. The transducer can be applied directly to the pipe through magnetic bases allowing for the quick measurement of the dynamic strains in many points of the pipe. The transducer signal can be read with the same commercial data collectors used for accelerometers. (author)

Strain measurement in concrete using embedded carbon roving-based sensors

International Nuclear Information System (INIS)

Quadflieg, Till; Gries, Thomas; Stolyarov, Oleg

2016-01-01

This paper presents the results of the application of carbon rovings as strain sensors for measuring the strain in concrete. In this work, three types of electrically conductive carbon roving with different characteristics were used. The possibility of using carbon rovings as a strain sensor is demonstrated via measurements in tensile and four point bending tests. The experimental setups and methods for measuring the electrical resistance of carbon roving in the roving and concrete are described. The results of the characterization of the electrical behavior as a function of strain of carbon rovings and concrete are presented and discussed. The obtained results indicate that the strain range of carbon rovings optimally corresponds to the strain range of concrete. This characteristic behavior makes the carbon rovings well suited for the use as strain sensors. A good correlation has been found between the electrical resistance-strain curve of the carbon roving and the measurements in the concrete.

Musculoskeletal Application of Ultrasound Elastography: Soft Tissue Lipoma

International Nuclear Information System (INIS)

Choi, Ja Young; Hong, Sung Hwan; Yoo, Hye Jin; Kim, Su Jin

2010-01-01

Real-time freehand elastography. Conventional ultrasonography (US) and real-time freehand US elastography were performed in nine patients (M:F = 4:5: mean age, 53 years: 29-64 years) with soft-tissue lipoma confirmed by surgical resection. The elastogram was color-coded by 256 scales according to the degree of strain induced by light compression. The relative strains for lipoma and surrounding soft tissue were measured and mean strains were examined by using a Wilcoxon signed rank test. The elastograms showed red to sky-blue color in all lipomas and predominantly black in surrounding soft tissue. The mean relative strain (±standard deviation) was 67.9±28.5, 77.1±25.3, 63.3±31.2, and 15.7±18.3 for total, intramuscular, and subcutaneous lipoma, and surrounding soft tissue, respectively. The mean strain of the lipoma was significantly higher than one of surrounding soft tissue (p = .008, Wilcoxon signed rank test). Real-time elastography yields characteristic elastographic features of soft tissue lipoma distinguishing from those of adjacent soft tissues

Measurement of tissue viscoelasticity with ultrasound

Science.gov (United States)

Greenleaf, J. F.; Alizad, A.

2017-02-01

Tissue properties such as elasticity and viscosity have been shown to be related to such tissue conditions as contraction, edema, fibrosis, and fat content among others. Magnetic Resonance Elastography has shown outstanding ability to measure the elasticity and in some cases the viscosity of tissues, especially in the liver, providing the ability to stage fibrotic liver disease similarly to biopsy. We discuss ultrasound methods of measuring elasticity and viscosity in tissues. Many of these methods are becoming widely available in the extant ultrasound machines distributed throughout the world. Some of the methods to be discussed are in the developmental stage. The advantages of the ultrasound methods are that the imaging instruments are widely available and that many of the viscoelastic measurements can be made during a short addition to the normal ultrasound examination time. In addition, the measurements can be made by ultrasound repetitively and quickly allowing evaluation of dynamic physiologic function in circumstances such as muscle contraction or artery relaxation. Measurement of viscoelastic tissue mechanical properties will become a consistent part of clinical ultrasound examinations in our opinion.

Strain measurement of abdominal aortic aneurysm with real-time 3D ultrasound speckle tracking.

Science.gov (United States)

Bihari, P; Shelke, A; Nwe, T H; Mularczyk, M; Nelson, K; Schmandra, T; Knez, P; Schmitz-Rixen, T

2013-04-01

Abdominal aortic aneurysm rupture is caused by mechanical vascular tissue failure. Although mechanical properties within the aneurysm vary, currently available ultrasound methods assess only one cross-sectional segment of the aorta. This study aims to establish real-time 3-dimensional (3D) speckle tracking ultrasound to explore local displacement and strain parameters of the whole abdominal aortic aneurysm. Validation was performed on a silicone aneurysm model, perfused in a pulsatile artificial circulatory system. Wall motion of the silicone model was measured simultaneously with a commercial real-time 3D speckle tracking ultrasound system and either with laser-scan micrometry or with video photogrammetry. After validation, 3D ultrasound data were collected from abdominal aortic aneurysms of five patients and displacement and strain parameters were analysed. Displacement parameters measured in vitro by 3D ultrasound and laser scan micrometer or video analysis were significantly correlated at pulse pressures between 40 and 80 mmHg. Strong local differences in displacement and strain were identified within the aortic aneurysms of patients. Local wall strain of the whole abdominal aortic aneurysm can be analysed in vivo with real-time 3D ultrasound speckle tracking imaging, offering the prospect of individual non-invasive rupture risk analysis of abdominal aortic aneurysms. Copyright © 2013 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

The challenges of measuring in vivo knee collateral ligament strains using ultrasound.

Science.gov (United States)

Slane, Laura C; Slane, Josh A; D'hooge, Jan; Scheys, Lennart

2017-08-16

Ultrasound-based methods have shown promise in their ability to characterize non-uniform deformations in large energy-storing tendons such as the Achilles and patellar tendons, yet applications to other areas of the body have been largely unexplored. The noninvasive quantification of collateral ligament strain could provide an important clinical metric of knee frontal plane stability, which is relevant in ligament injury and for measuring outcomes following total knee arthroplasty. In this pilot cadaveric experiment, we investigated the possibility of measuring collateral ligament strain with our previously validated speckle-tracking approach, but encountered a number of challenges during both data acquisition and processing. Given the clinical interest in this type of tool, and the fact that this is a developing area of research, the goal of this article is to transparently describe this pilot study, both in terms of methods and results, while also identifying specific challenges to this work and areas for future study. Some challenges faced relate generally to speckle-tracking of soft tissues (e.g. the limitations of using a 2D imaging modality to characterize 3D motion), while others are specific to this application (e.g. the small size and complex anatomy of the collateral ligaments). This work illustrates a clear need for additional studies, particularly relating to the collection of ground-truth data and more thorough validation work. These steps will be critical prior to the translation of ultrasound-based measures of collateral ligament strains into the clinic. Copyright © 2017 Elsevier Ltd. All rights reserved.

Measurement of Strain and Strain Rate during the Impact of Tennis Ball Cores

Directory of Open Access Journals (Sweden)

Ben Lane

2018-03-01

Full Text Available The aim of this investigation was to establish the strains and strain rates experienced by tennis ball cores during impact to inform material characterisation testing and finite element modelling. Three-dimensional surface strains and strain rates were measured using two high-speed video cameras and corresponding digital image correlation software (GOM Correlate Professional. The results suggest that material characterisation testing to a maximum strain of 0.4 and a maximum rate of 500 s−1 in tension and to a maximum strain of −0.4 and a maximum rate of −800 s−1 in compression would encapsulate the demands placed on the material during impact and, in turn, define the range of properties required to encapsulate the behavior of the material during impact, enabling testing to be application-specific and strain-rate-dependent properties to be established and incorporated in finite element models.

Dynamic strain measurements in a sliding microstructured contact

International Nuclear Information System (INIS)

Bennewitz, Roland; David, Jonathan; Lannoy, Charles-Francois de; Drevniok, Benedict; Hubbard-Davis, Paris; Miura, Takashi; Trichtchenko, Olga

2008-01-01

A novel experiment is described which measures the tangential strain development across the contact between a PDMS (polydimethylsiloxane) block and a glass surface during the initial stages of sliding. The surface of the PDMS block has been microfabricated to take the form of a regular array of pyramidal tips at 20 μm separation. Tangential strain is measured by means of light scattering from the interface between the block and surface. Three phases are observed in all experiments: initial shear deformation of the whole PDMS block, a pre-sliding tangential compression of the tip array with stepwise increase of the compressive strain, and sliding in stick-slip movements as revealed by periodic variation of the strain. The stick-slip sliding between the regular tip array and the randomly rough counter surface always takes on the periodicity of the tip array. The fast slip can cause either a sudden increase or a sudden decrease in compressive strain

Measured Strain of Nb3Sn Coils During Excitation and Quench

International Nuclear Information System (INIS)

Caspi, S.; Bartlett, S.E.; Dietderich, D.R.; Ferracin, P.; Gourlay, S.A.; Hannaford, C.R.; Hafalia, A.R.; Lietzke, S.; Mattafirri, M.; Nyman, M.; Sabbi, G.

2005-01-01

The strain in a high field Nb 3 Sn coil was measured during magnet assembly, cool-down, excitation and spot heater quenches. Strain was measured with a full bridge strain gauge mounted directly over the turns and impregnated with the coil. Two such coils were placed in a ''common coil'' fashion capable of reaching 11T at 4.2K. The measured steady state strain in the coil is compared with results obtained using the FEM code ANSYS. During quenches, the transient strain (due to temperature rise) was also measured and compared with the calculated mechanical time response to a quench

Resonant tunneling measurements of size-induced strain relaxation

Science.gov (United States)

Akyuz, Can Deniz

Lattice mismatch strain available in such semiconductor heterostructures as Si/SiGe or GaAs/AlGaAs can be employed to alter the electronic and optoelectronic properties of semiconductor structures and devices. When deep submicron structures are fabricated from strained material, strained layers relax by sidewall expansion giving rise to size- and geometry-dependent strain gradients throughout the structure. This thesis describes a novel experimental technique to probe the size-induced strain relaxation by studying the tunneling current characteristics of strained p-type Si/SiGe resonant tunneling diodes. Our current-voltage measurements on submicron strained p-Si/SiGe double- and triple-barrier resonant tunneling structures as a function of device diameter, D, provide experimental access to both the average strain relaxation (which leads to relative shifts in the tunneling current peak positions) and strain gradients (which give rise to a fine structure in the current peaks due to inhomogeneous strain-induced lateral quantization). We find that strain relaxation is significant, with a large fraction of the strain energy relaxed on average in D ≤ 0.25 m m devices. Further, the in-plane potentials that arise from inhomogeneous strain gradients are large. In the D ˜ 0.2 m m devices, the corresponding lateral potentials are approximately parabolic exceeding ˜ 25 meV near the perimeter. These potentials create discrete hole states in double-barrier structures (single well), and coupled hole states in triple-barrier structures (two wells). Our results are in excellent agreement with finite-element strain calculations in which the strained layers are permitted to relax to a state of minimum energy by sidewall expansion. Size-induced strain relaxation will undoubtedly become a serious technological issue once strained devices are scaled down to the deep submicron regime. Interestingly, our calculations predict and our measurements are consistent with the appearance of

Lattice strain measurements on sandstones under load using neutron diffraction

Science.gov (United States)

Frischbutter, A.; Neov, D.; Scheffzük, Ch.; Vrána, M.; Walther, K.

2000-11-01

Neutron diffraction methods (both time-of-flight- and angle-dispersive diffraction) are applied to intracrystalline strain measurements on geological samples undergoing uniaxial increasing compressional load. The experiments were carried out on Cretaceous sandstones from the Elbezone (East Germany), consisting of >95% quartz which are bedded but without crystallographic preferred orientation of quartz. From the stress-strain relation the Young's modulus for our quartz sample was determined to be (72.2±2.9) GPa using results of the neutron time-of-flight method. The influence of different kinds of bedding in sandstones (laminated and convolute bedding) could be determined. We observed differences of factor 2 (convolute bedding) and 3 (laminated bedding) for the elastic stiffness, determined with angle dispersive neutron diffraction (crystallographic strain) and with strain gauges (mechanical strain). The data indicate which geological conditions may influence the stress-strain behaviour of geological materials. The influence of bedding on the stress-strain behaviour of a laminated bedded sandstone was indicated by direct residual stress measurements using neutron time-of-flight diffraction. The measurements were carried out six days after unloading the sample. Residual strain was measured for three positions from the centre to the periphery and within two radial directions of the cylinder. We observed that residual strain changes from extension to compression in a different manner for two perpendicular directions of the bedding plane.

X-ray strain tensor imaging: FEM simulation and experiments with a micro-CT.

Science.gov (United States)

Kim, Jae G; Park, So E; Lee, Soo Y

2014-01-01

In tissue elasticity imaging, measuring the strain tensor components is necessary to solve the inverse problem. However, it is impractical to measure all the tensor components in ultrasound or MRI elastography because of their anisotropic spatial resolution. The objective of this study is to compute 3D strain tensor maps from the 3D CT images of a tissue-mimicking phantom. We took 3D micro-CT images of the phantom twice with applying two different mechanical compressions to it. Applying the 3D image correlation technique to the CT images under different compression, we computed 3D displacement vectors and strain tensors at every pixel. To evaluate the accuracy of the strain tensor maps, we made a 3D FEM model of the phantom, and we computed strain tensor maps through FEM simulation. Experimentally obtained strain tensor maps showed similar patterns to the FEM-simulated ones in visual inspection. The correlation between the strain tensor maps obtained from the experiment and the FEM simulation ranges from 0.03 to 0.93. Even though the strain tensor maps suffer from high level noise, we expect the x-ray strain tensor imaging may find some biomedical applications such as malignant tissue characterization and stress analysis inside the tissues.

[Effect of elastic strain rate ratio method and virtual touch tissue quantification on the diagnosis of breast masses].

Science.gov (United States)

Gong, LiJie; He, Yan; Tian, Peng; Yan, Yan

2016-07-01

To determine the effect of elastic strain rate ratio method and virtual touch tissue quantification (VTQ) on the diagnosis of breast masses.
 Sixty female patients with breast cancer, who received surgical treatment in Daqing Oilfield General Hospital, were enrolled. All patients signed the informed consent paperwork and they were treated by routine ultrasound examination, compression elastography (CE) examination, and VTQ examination in turn. Strain ratio (SR) was checked by CE and shear wave velocity (SWV) value was measured by VTQ. The diagnostic values of different methods were evaluated by receiver operating characteristic (ROC) curves in the diagnosis of benign and malignant breast tumors.
 The maximum diameter and SWV value of the benign tumors were lower than those of the malignant tumors, and the SR ratio of benign masses was higher than that of malignant tumors (Pbreast mass than that used alone.

Utilizing Photogrammetry and Strain Gage Measurement to Characterize Pressurization of an Inflatable Module

Science.gov (United States)

Valle, Gerard D.; Selig, Molly; Litteken, Doug; Oliveras, Ovidio

2012-01-01

This paper documents the integration of a large hatch penetration into an inflatable module. This paper also documents the comparison of analytical load predictions with measured results utilizing strain measurement. Strain was measured by utilizing photogrammetric measurement and through measurement obtained from strain gages mounted to selected clevises that interface with the structural webbings. Bench testing showed good correlation between strain measurement obtained from an extensometer and photogrammetric measurement especially after the fabric has transitioned through the low load/high strain region of the curve. Test results for the full-scale torus showed mixed results in the lower load and thus lower strain regions. Overall strain, and thus load, measured by strain gages and photogrammetry tracked fairly well with analytical predictions. Methods and areas of improvements are discussed.

Unsteady Aerodynamic Force Sensing from Measured Strain

Science.gov (United States)

Pak, Chan-Gi

2016-01-01

A simple approach for computing unsteady aerodynamic forces from simulated measured strain data is proposed in this study. First, the deflection and slope of the structure are computed from the unsteady strain using the two-step approach. Velocities and accelerations of the structure are computed using the autoregressive moving average model, on-line parameter estimator, low-pass filter, and a least-squares curve fitting method together with analytical derivatives with respect to time. Finally, aerodynamic forces over the wing are computed using modal aerodynamic influence coefficient matrices, a rational function approximation, and a time-marching algorithm. A cantilevered rectangular wing built and tested at the NASA Langley Research Center (Hampton, Virginia, USA) in 1959 is used to validate the simple approach. Unsteady aerodynamic forces as well as wing deflections, velocities, accelerations, and strains are computed using the CFL3D computational fluid dynamics (CFD) code and an MSC/NASTRAN code (MSC Software Corporation, Newport Beach, California, USA), and these CFL3D-based results are assumed as measured quantities. Based on the measured strains, wing deflections, velocities, accelerations, and aerodynamic forces are computed using the proposed approach. These computed deflections, velocities, accelerations, and unsteady aerodynamic forces are compared with the CFL3D/NASTRAN-based results. In general, computed aerodynamic forces based on the lifting surface theory in subsonic speeds are in good agreement with the target aerodynamic forces generated using CFL3D code with the Euler equation. Excellent aeroelastic responses are obtained even with unsteady strain data under the signal to noise ratio of -9.8dB. The deflections, velocities, and accelerations at each sensor location are independent of structural and aerodynamic models. Therefore, the distributed strain data together with the current proposed approaches can be used as distributed deflection

Utilizing Photogrammetry and Strain Gage Measurement to Characterize Pressurization of Inflatable Modules

Science.gov (United States)

Mohammed, Anil

2011-01-01

This paper focuses on integrating a large hatch penetration into inflatable modules of various constructions. This paper also compares load predictions with test measurements. The strain was measured by utilizing photogrammetric methods and strain gages mounted to select clevises that interface with the structural webbings. Bench testing showed good correlation between strain data collected from an extensometer and photogrammetric measurements, even when the material transitioned from the low load to high load strain region of the curve. The full-scale torus design module showed mixed results as well in the lower load and high strain regions. After thorough analysis of photogrammetric measurements, strain gage measurements, and predicted load, the photogrammetric measurements seem to be off by a factor of two.

Non-Destructive Measurement of Residual Strain in Connecting Rods Using Neutrons

Energy Technology Data Exchange (ETDEWEB)

Ikeda, Tomohiro [Honda R& D; Bunn, Jeffrey R. [ORNL; Fancher, Christopher M. [ORNL; Seid, Alan [Honda R& D; Motani, Ryuta [Honda R& D; Matsuda, Hideki [Honda R& D; Okayama, Tatsuya [Honda R& D

2018-04-01

Increasing the strength of materials is effective in reducing weight and boosting structural part performance, but there are cases in where the residual strain generated during the process of manufacturing of high-strength materials results in a decline of durability. It is therefore important to understand how the residual strain in a manufactured component changes due to processing conditions. In the case of a connecting rod, because the strain load on the connecting rod rib sections is high, it is necessary to clearly understand the distribution of strain in the ribs. However, because residual strain is generally measured by using X-ray diffractometers or strain gauges, measurements are limited to the surface layer of the parts. Neutron beams, however, have a higher penetration depth than X-rays, allowing for strain measurement in the bulk material. The research discussed within this paper consists of non-destructive residual strain measurements in the interior of connecting rods using the 2nd Generation Neutron Residual Stress Mapping Facility (NRSF2) at Oak Ridge National Laboratory, measuring the Fe (211) diffraction peak position of the ferrite phase. The interior strain distribution of connecting rod, which prepared under different manufacturing processes, was revealed. By the visualization of interior strains, clear understandings of differences in various processing conditions were obtained. In addition, it is known that the peak width, which is also obtained during measurement, is suggestive of the size of crystallites in the structure; however the peak width can additionally be caused by microstresses and material dislocations.

Engineering related neutron diffraction measurements probing strains, texture and microstructure

Energy Technology Data Exchange (ETDEWEB)

Clausen, Bjorn [Los Alamos National Laboratory; Brown, Donald W [Los Alamos National Laboratory; Tome, Carlos N [Los Alamos National Laboratory; Balogh, Levente [Los Alamos National Laboratory; Vogel, Sven C [Los Alamos National Laboratory

2010-01-01

Neutron diffraction has been used for engineering applications for nearly three decades. The basis of the technique is powder diffraction following Bragg's Law. From the measured diffraction patterns information about internal, or residual, strain can be deduced from the peak positions, texture information can be extracted from the peak intensities, and finally the peak widths can provide information about the microstructure, e.g. dislocation densities and grain sizes. The strains are measured directly from changes in lattice parameters, however, in many cases it is non-trivial to determine macroscopic values of stress or strain from the measured data. The effects of intergranular strains must be considered, and combining the neutron diffraction measurements with polycrystal deformation modeling has proven invaluable in determining the overall stress and strain values of interest in designing and dimensioning engineering components. Furthelmore, the combined use of measurements and modeling has provided a tool for elucidating basic material properties, such as critical resolved shear stresses for the active deformation modes and their evolution as a function of applied deformation.

Electro optical system to measure strains at high temperature

Science.gov (United States)

Sciammarella, Cesar A.

1991-12-01

The measurement of strains at temperatures of the order of 1000 C has become a very important field of research. Technological advances in areas such as the analysis of high speed aircraft structures and high efficiency thermal engines require operational temperatures of this order of magnitude. Current techniques for the measurement of strains, such as electrical strain gages, are at the limit of their useful range and new methods need to be developed. Optical techniques are very attractive in this type of application because of their noncontacting nature. Holography is of particular interest because a minimal preparation of the surfaces is required. Optoelectronics holography is specially suited for this type of application, from the point of view of industrial use. There are a number of technical problems that need to be overcome to measure strains using holographic interferometry at high temperatures. Some of these problems are discussed, and solutions are given. A specimen instrumented with high temperature strains gages is used to compare the results of both technologies.

The application of a 3 dimensional image scanner to the strain measurement

International Nuclear Information System (INIS)

Mazda, Taiji; Ogawa, Hiroshi; Suzuki, Michiaki; Nakano, Yasuo.

1993-01-01

A large strain measuring method for a laminated seismic isolation rubber, which will be introduced to reactor buildings of the Demonstration Fast Breeder Reactor (DFBR), was developed. With using strain gages, it is difficult to measure the large strain under the large displacement condition. With using the optical instruments, it is also impossible to measure the strain of a 3 dimensional object. We developed a new measuring method in which strain is calculated from a 3 dimensional deformation with using a 3 dimensional image scanner. This method is noncontact measuring method, and it can measure the strain of a 3 dimensional object under the large deformation. This work is one part of 'The Development of FBR Seismic Isolation system' operated by Central Research Institute of Electric Power Industry. (author)

Tattoo-Like Strain Gauges Based on Silicon Nano-Membranes

Science.gov (United States)

Lu, Nanshu

2012-02-01

This talk reports the in vivo measurement of tissue deformation through adhesive-free, conformable lamination of a tattoo-like elastic strain gauge consisted of piezoresistive silicon nano-membranes strategically integrated with tissue-like elastomeric substrates. The mechanical deformation in soft tissues cannot yet be directly quantified due to the lack of enabling tools. While stiff strain gauges for structural health monitoring have long existed, biological tissues are soft, curvilinear and highly deformable in contrast to civil or aerospace structures. An ultra-thin, ultra-soft, tattoo-like strain gauge that can conform to the convoluted surface of human body and stay attached during locomotion will be able to directly quantify tissue deformation without affecting the mechanical behavior of the tissue. While single crystalline silicon is known to have the highest gauge factor and best elastic response, it is intrinsically stiff and brittle. To achieve strain gauges with high compliance, high stretchability and reasonable sensitivity, single crystalline silicon nano-membranes will be transfer-printed onto polymeric support through carefully engineered stamps. The thickness and length of the Si strip will be chosen according to theoretical and numerical mechanics analysis which takes into account for the tradeoff between stretchability and sensitivity.

A new strain gage method for measuring the contractile strain ratio of Zircaloy tubing

International Nuclear Information System (INIS)

Hwang, S.K.; Sabol, G.P.

1988-01-01

An improved strain gage method for determining the contractile strain ratio (CSR) of Zircaloy tubing was developed. The new method consists of a number of load-unload cyclings at approximately 0.2% plastic strain interval. With this method the CSR of Zircaloy-4 tubing could be determined accurately because it was possible to separate the plastic strains from the elastic strain involvement. The CSR values determined by use of the new method were in good agreement with those calculated from conventional post-test manual measurements. The CSR of the tubing was found to decrease with the amount of deformation during testing because of uneven plastic flow in the gage section. A new technique of inscribing gage marks by use of a YAG laser is discussed. (orig.)

Characterization of inter-tissue and inter-strain variability of TCE glutathione conjugation metabolites DCVG, DCVC, and NAcDCVC in the mouse.

Science.gov (United States)

Luo, Yu-Syuan; Furuya, Shinji; Chiu, Weihsueh; Rusyn, Ivan

2018-01-01

Trichloroethylene (TCE) is a ubiquitous environmental toxicant that is a liver and kidney carcinogen. Conjugation of TCE with glutathione (GSH) leads to formation of nepthrotoxic and mutagenic metabolites postulated to be critical for kidney cancerdevelopment; however, relatively little is known regarding their tissue levels as previous analytical methods for their detection lacked sensitivity. Here, an LC-MS/MS-based method for simultaneous detection of S-(1,2-dichlorovinyl)-glutathione (DCVG), S-(1,2-dichlorovinyl)-L-cysteine (DCVC), and N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC) in multiple mouse tissues was developed. This analytical method is rapid, sensitive (limits of detection (LOD) 3-30 fmol across metabolites and tissues), and robust to quantify all three metabolites in liver, kidneys, and serum. The method was used to characterize inter-tissue and inter-strain variability in formation of conjugative metabolites of TCE. Single oral dose of TCE (24, 240 or 800 mg/kg) was administered to male mice from 20 inbred strains of Collaborative Cross. Inter-strain variability in the levels of DCVG, DCVC, and NAcDCVC (GSD = 1.6-2.9) was observed. Whereas NAcDCVC was distributed equally among analyzed tissues, highest levels of DCVG were detected in liver and DCVC in kidneys. Evidence indicated that inter-strain variability in conjugative metabolite formation of TCE might affect susceptibility to adverse health effects and that this method might aid in filling data gaps in human health assessment of TCE.

Strain Measurement System Developed for Biaxially Loaded Cruciform Specimens

Science.gov (United States)

Krause, David L.

2000-01-01

A new extensometer system developed at the NASA Glenn Research Center at Lewis Field measures test area strains along two orthogonal axes in flat cruciform specimens. This system incorporates standard axial contact extensometers to provide a cost-effective high-precision instrument. The device was validated for use by extensive testing of a stainless steel specimen, with specimen temperatures ranging from room temperature to 1100 F. In-plane loading conditions included several static biaxial load ratios, plus cyclic loadings of various waveform shapes, frequencies, magnitudes, and durations. The extensometer system measurements were compared with strain gauge data at room temperature and with calculated strain values for elevated-temperature measurements. All testing was performed in house in Glenn's Benchmark Test Facility in-plane biaxial load frame.

A production of non-strain spacing of lattice planes measurement equipment and a measurement of general structure material

International Nuclear Information System (INIS)

Minakawa, Nobuaki; Moriai, Atsushi; Morii, Yukio

2001-01-01

It is necessary to determine Δd/d in the internal stress measurement by the neutron diffraction method. Therefore, in case the non-strain spacing of lattice planes d 0 (hkl) is measured using bulk material, even though it does and attaches in a sample table length or every width and it is performing the diffraction measurement, it is difficult to determine for a true non-strain spacing of lattice planes by a processing strain, the grain-orientation, etc. It is available for the infinite thing spacing of lattice planes near non-strain condition to be measured by doing random rotation for bulk material in a beam center, and measuring an average spacing of lattice planes. Practical non-strain spacing of lattice planes measurement equipment was made, and the measurement was performed about much structure material. (author)

Measuring systolic ankle and toe pressure using the strain gauge technique--a comparison study between mercury and indium-gallium strain gauges

DEFF Research Database (Denmark)

Broholm, Rikke; Wiinberg, Niels; Simonsen, Lene

2014-01-01

BACKGROUND: Measurement of the ankle and toe pressures are often performed using a plethysmograph, compression cuffs and a strain gauge. Usually, the strain gauge contains mercury but other alternatives exist. From 2014, the mercury-containing strain gauge will no longer be available in the Europ......BACKGROUND: Measurement of the ankle and toe pressures are often performed using a plethysmograph, compression cuffs and a strain gauge. Usually, the strain gauge contains mercury but other alternatives exist. From 2014, the mercury-containing strain gauge will no longer be available...... in the European Union. The aim of this study was to compare an indium-gallium strain gauge to the established mercury-containing strain gauge. METHODS: Consecutive patients referred to the Department of Clinical Physiology and Nuclear Medicine at Bispebjerg and Frederiksberg Hospitals for measurements of systolic...... ankle and toe pressures volunteered for the study. Ankle and toe pressures were measured twice with the mercury and the indium-gallium strain gauge in random order. Comparison of the correlation between the mean pressure using the mercury and the indium-gallium device and the difference between the two...

The use of pulsed neutron diffraction to measure strain in composites

International Nuclear Information System (INIS)

Bourke, M.A.M.; Goldstone, J.A.; Shi, N.; Gray, G.T. III; James, M.R.

1994-01-01

Neutron diffraction is a technique for measuring strain in crystalline materials. It is non destructive, phase discriminatory and more penetrating than X rays. Pulsed neutron sources (in contrast with steady state reactor sources) are particularly appropriate for examining heterogeneous materials or for recording the polycrystalline response of all lattice reflections. Several different aspects of composite behavior can be characterized and examples are given of residual strain measurements, strain relaxation during heating, applied loading, and determination of the strain distribution function

Development of shearography for surface strain measurement of non planar objects

International Nuclear Information System (INIS)

Groves, Roger Michael

2001-01-01

The subject of this thesis is the development of optical instrumentation for surface strain measurement of non-planar objects. The speckle interferometry technique of shearography is used to perform quantitative measurements of surface strain on non-planar objects and to compensate these measurements for the errors that are due to the shape and slope of the object. Shearography is an optical technique that is usually used for defect location and for qualitative strain characterisation. In this thesis a multi-component shearography system is described that can measure the six components of displacement gradient. From these measurements the surface strain can be fully characterised. For non-planar objects an error is introduced into the displacement gradient measurement due to the variation of the sensitivity vector across the field of view and the variation in the magnitude of applied shear due to the curvature of the object surface. To correct for these errors requires a knowledge of the slope and shape of the object. Shearography may also be used to measure object slope and shape by a source displacement technique. Therefore slope, shape and surface strain may be measured using the same optical system. The thesis describes a method of multiplexing the shear direction using polarisation switching, a method of measuring the source position using shadow Moire and the shearography source displacement technique for measuring the surface slope and shape of objects. The multi-component shearography system is used to perform measurements of the six components of surface strain, on an industrial component, with a correction applied for errors due to the shape and slope of the object. (author)

Infection studies with two highly pathogenic avian influenza strains (Vietnamese and Indonesian) in Pekin ducks (Anas platyrhynchos), with particular reference to clinical disease, tissue tropism and viral shedding.

Science.gov (United States)

Bingham, John; Green, Diane J; Lowther, Sue; Klippel, Jessica; Burggraaf, Simon; Anderson, Danielle E; Wibawa, Hendra; Hoa, Dong Manh; Long, Ngo Thanh; Vu, Pham Phong; Middleton, Deborah J; Daniels, Peter W

2009-08-01

Pekin ducks were infected by the mucosal route (oral, nasal, ocular) with one of two strains of Eurasian lineage H5N1 highly pathogenic avian influenza virus: A/Muscovy duck/Vietnam/453/2004 and A/duck/Indramayu/BBVW/109/2006 (from Indonesia). Ducks were killed humanely on days 1, 2, 3, 5 and 7 after challenge, or whenever morbidity was severe enough to justify euthanasia. Morbidity was recorded by observation of clinical signs and cloacal temperatures; the disease was characterized by histopathology; tissue tropism was studied by immunohistochemistry and virus titration on tissue samples; and viral shedding patterns were determined by virus isolation and titration of oral and cloacal swabs. The Vietnamese strain caused severe morbidity with fever and depression; the Indonesian strain caused only transient fever. Both viruses had a predilection for a similar range of tissue types, but the quantity of tissue antigen and tissue virus titres were considerably higher with the Vietnamese strain. The Vietnamese strain caused severe myocarditis and skeletal myositis; both strains caused non-suppurative encephalitis and a range of other inflammatory reactions of varying severity. The principal epithelial tissue infected was that of the air sacs, but antigen was not abundant. Epithelium of the turbinates, trachea and bronchi had only rare infection with virus. Virus was shed from both the oral and cloacal routes; it was first detected 24 h after challenge and persisted until day 5 after challenge. The higher prevalence of virus from swabs from ducks infected with the Vietnamese strain indicates that this strain may be more adapted to ducks than the Indonesia strain.

Murine strain differences and the effects of zinc on cadmium concentrations in tissues after acute cadmium exposure

Energy Technology Data Exchange (ETDEWEB)

King, L.M. [ARS USDA, Germplasm and Gamete Physiology Lab., Beltsville, MD (United States); Anderson, M.B. [Dept. of Anatomy, Tulane Univ. School of Medicine, New Orleans, LA (United States); Sikka, S.C. [Dept. of Urology, Tulane Univ. School of Medicine, New Orleans, LA (United States); George, W.J. [Dept. of Pharmacology, Tulane Univ. School of Medicine, New Orleans, LA (United States)

1998-10-01

The role of strain differences in cadmium tissue distribution was studied using sensitive (129/J) and resistant (A/J) mice. These murine strains have previously been shown to differ in their susceptibility to cadmium-induced testicular toxicity. Cadmium concentration was measured in testis, epididymis, seminal vesicle, liver, and kidney at 24 h after cadmium chloride exposure (4, 10, and 20 {mu}mol/kg CdCl{sub 2}). The 129/J mice exhibited a significant increase in cadmium concentration in testis, epididymis, and seminal vesicle at all cadmium doses used, compared to A/J mice. However, cadmium concentrations in liver and kidney were not different between the strains, at any dose, indicating that cadmium uptake is similar in these organs at 24 h. These murine strains demonstrate similar hepatic and renal cadmium uptake but significantly different cadmium accumulation in the reproductive organs at 24 h. The mechanism of the protective effect of zinc on cadmium toxicity was studied by assessing the impact of zinc acetate (ZnAc) treatment on cadmium concentrations in 129/J mice after 24 h. Zinc pretreatment (250 {mu}mol/kg ZnAc), given 24 h prior to 20 {mu}mol/kg CdCl{sub 2} administration, significantly decreased the amount of cadmium in the testis, epididymis, and seminal vesicle of 129/J mice, and significantly increased the cadmium content of the liver after 24 h. Cadmium levels in the kidney were unaffected at this time. Zinc pretreatment also prevented the cadmium-induced decrease in testicular sperm concentration and epididymal sperm motility seen in 129/J mice. These findings suggest that the differences in the two murine strains may be attributed partly to the differential accumulation of cadmium in murine gonads. This may be caused by strain differences in the specificity of cadmium transport mechanisms. The protective role of zinc in cadmium-induced testicular toxicity in the sensitive strain may be due to an interference in the cadmium uptake by susceptible

Tissue tropisms in group A Streptococcus: what virulence factors distinguish pharyngitis from impetigo strains?

Science.gov (United States)

Bessen, Debra E

2016-06-01

Group A streptococci (GAS) are a common cause of pharyngitis and impetigo, and distinct throat strains and skin strains have been long recognized. This review aims to describe recent advances in molecular differences between throat and skin strains, and the pathogenic mechanisms used by virulence factors that may distinguish between these two groups. Recent findings include a new typing scheme for GAS strains based on sequence clusters of genes encoding the entire surface-exposed portion of M protein; correlations between emm-based typing schemes, clinical disease and surface adhesins; covalent bond formation mediated by GAS pili and other adhesins in binding to host ligands; a key role for superantigens in oropharyngeal infection via binding major histocompatibility complex class II antigen; and migration of GAS-specific Th17 cells from the upper respiratory tract to the brain, which may be relevant to autoimmune sequelae. The gap between molecular markers of disease (correlation) and virulence mechanisms (causation) in the establishment of tissue tropisms for GAS infection currently remains wide, but the gap also continues to narrow. Whole genome sequencing combined with mutant construction and improvements in animal models for oropharyngeal infection by GAS may help pave the way for new discoveries.

From Measurements Errors to a New Strain Gauge Design

DEFF Research Database (Denmark)

Mikkelsen, Lars Pilgaard; Zike, Sanita; Salviato, Marco

2015-01-01

Significant over-prediction of the material stiffness in the order of 1-10% for polymer based composites has been experimentally observed and numerical determined when using strain gauges for strain measurements instead of non-contact methods such as digital image correlation or less stiff method...

Exploring the mechanical behavior of degrading swine neural tissue at low strain rates via the fractional Zener constitutive model.

Science.gov (United States)

Bentil, Sarah A; Dupaix, Rebecca B

2014-02-01

The ability of the fractional Zener constitutive model to predict the behavior of postmortem swine brain tissue was examined in this work. Understanding tissue behavior attributed to degradation is invaluable in many fields such as the forensic sciences or cases where only cadaveric tissue is available. To understand how material properties change with postmortem age, the fractional Zener model was considered as it includes parameters to describe brain stiffness and also the parameter α, which quantifies the viscoelasticity of a material. The relationship between the viscoelasticity described by α and tissue degradation was examined by fitting the model to data collected in a previous study (Bentil, 2013). This previous study subjected swine neural tissue to in vitro unconfined compression tests using four postmortem age groups (week). All samples were compressed to a strain level of 10% using two compressive rates: 1mm/min and 5mm/min. Statistical analysis was used as a tool to study the influence of the fractional Zener constants on factors such as tissue degradation and compressive rate. Application of the fractional Zener constitutive model to the experimental data showed that swine neural tissue becomes less stiff with increased postmortem age. The fractional Zener model was also able to capture the nonlinear viscoelastic features of the brain tissue at low strain rates. The results showed that the parameter α was better correlated with compressive rate than with postmortem age. © 2013 Published by Elsevier Ltd.

High-resolution optical polarimetric elastography for measuring the mechanical properties of tissue

Science.gov (United States)

Hudnut, Alexa W.; Armani, Andrea M.

2018-02-01

Traditionally, chemical and molecular markers have been the predominate method in diagnostics. Recently, alternate methods of determining tissue and disease characteristics have been proposed based on testing the mechanical behavior of biomaterials. Existing methods for performing elastography measurements, such as atomic force microscopy, compression testing, and ultrasound elastography, require either extensive sample processing or have poor resolution. In the present work, we demonstrate an optical polarimetric elastography device to characterize the mechanical properties of salmon skeletal muscle. A fiber-coupled 1550nm laser paired with an optical polarizer is used to create a fiber optic sensing region. By measuring the change in polarization from the initial state to the final state within the fiber sensing region with a polarimeter, the loading-unloading curves can be determined for the biomaterial. The device is used to characterize the difference between samples with a range of collagen membranes. The loading-unloading curves are used to determine the change in polarization phase and energy loss of the samples at 10%, 20% and 30% strain. As expected, the energy loss is a better metric for measuring the mechanical properties of the tissues because it incorporates the entire loading-unloading curve rather than a single point. Using this metric, it is demonstrated the device can repeatedly differentiate between the different membrane configurations.

Effects of gauge volume on pseudo-strain induced in strain measurement using time-of-flight neutron diffraction

International Nuclear Information System (INIS)

Suzuki, Hiroshi; Harjo, Stefanus; Abe, Jun; Xu, Pingguang; Aizawa, Kazuya; Akita, Koichi

2013-01-01

Spurious or pseudo-strains observed in time-of-flight (TOF) neutron diffraction due to neutron attenuation, surface-effects and a strain distribution within the gauge volume were investigated. Experiments were carried out on annealed and bent ferritic steel bars to test these effects. The most representative position in the gauge volume corresponds to the neutron-weighted center of gravity (ncog), which takes into account variations in intensity within the gauge volume due to neutron attenuation and/or absence of material in the gauge volume. The average strain in the gauge volume was observed to be weighted towards the ncog position but following an increase in the size of the gauge volume the weighted average strain was changed because of the change in the ncog position when a strain gradient appeared within the gauge volume. On the other hand, typical pseudo-strains, which are well known, did appear in through-surface strain measurements when the gauge volume was incompletely filled by the sample. Tensile pseudo-strains due to the surface-effect increased near the sample surface and exhibited a similar trend regardless of the size of the gauge volume, while the pseudo-strains increased faster for the smaller gauge volume. Furthermore, a pseudo-strain due to a change in the ncog position was observed even when the gauge volume was perfectly filled in the sample, and it increased with an increase in the size of the gauge volume. These pseudo-strains measured were much larger than those simulated by the conventional modeling, whereas they were simulated by taking into account an incident neutron beam divergence additionally in the model. Therefore, the incident divergence of the incident neutron beam must be carefully designed to avoid pseudo-strains in time-of-flight neutron diffractometry

On the Rule of Mixtures for Predicting Stress-Softening and Residual Strain Effects in Biological Tissues and Biocompatible Materials

Directory of Open Access Journals (Sweden)

Alex Elías-Zúñiga

2014-01-01

Full Text Available In this work, we use the rule of mixtures to develop an equivalent material model in which the total strain energy density is split into the isotropic part related to the matrix component and the anisotropic energy contribution related to the fiber effects. For the isotropic energy part, we select the amended non-Gaussian strain energy density model, while the energy fiber effects are added by considering the equivalent anisotropic volumetric fraction contribution, as well as the isotropized representation form of the eight-chain energy model that accounts for the material anisotropic effects. Furthermore, our proposed material model uses a phenomenological non-monotonous softening function that predicts stress softening effects and has an energy term, derived from the pseudo-elasticity theory, that accounts for residual strain deformations. The model’s theoretical predictions are compared with experimental data collected from human vaginal tissues, mice skin, poly(glycolide-co-caprolactone (PGC25 3-0 and polypropylene suture materials and tracheal and brain human tissues. In all cases examined here, our equivalent material model closely follows stress-softening and residual strain effects exhibited by experimental data.

On the Rule of Mixtures for Predicting Stress-Softening and Residual Strain Effects in Biological Tissues and Biocompatible Materials

Science.gov (United States)

Elías-Zúñiga, Alex; Baylón, Karen; Ferrer, Inés; Serenó, Lídia; Garcia-Romeu, Maria Luisa; Bagudanch, Isabel; Grabalosa, Jordi; Pérez-Recio, Tania; Martínez-Romero, Oscar; Ortega-Lara, Wendy; Elizalde, Luis Ernesto

2014-01-01

In this work, we use the rule of mixtures to develop an equivalent material model in which the total strain energy density is split into the isotropic part related to the matrix component and the anisotropic energy contribution related to the fiber effects. For the isotropic energy part, we select the amended non-Gaussian strain energy density model, while the energy fiber effects are added by considering the equivalent anisotropic volumetric fraction contribution, as well as the isotropized representation form of the eight-chain energy model that accounts for the material anisotropic effects. Furthermore, our proposed material model uses a phenomenological non-monotonous softening function that predicts stress softening effects and has an energy term, derived from the pseudo-elasticity theory, that accounts for residual strain deformations. The model’s theoretical predictions are compared with experimental data collected from human vaginal tissues, mice skin, poly(glycolide-co-caprolactone) (PGC25 3-0) and polypropylene suture materials and tracheal and brain human tissues. In all cases examined here, our equivalent material model closely follows stress-softening and residual strain effects exhibited by experimental data. PMID:28788466

Micro/Nano-scale Strain Distribution Measurement from Sampling Moiré Fringes.

Science.gov (United States)

Wang, Qinghua; Ri, Shien; Tsuda, Hiroshi

2017-05-23

This work describes the measurement procedure and principles of a sampling moiré technique for full-field micro/nano-scale deformation measurements. The developed technique can be performed in two ways: using the reconstructed multiplication moiré method or the spatial phase-shifting sampling moiré method. When the specimen grid pitch is around 2 pixels, 2-pixel sampling moiré fringes are generated to reconstruct a multiplication moiré pattern for a deformation measurement. Both the displacement and strain sensitivities are twice as high as in the traditional scanning moiré method in the same wide field of view. When the specimen grid pitch is around or greater than 3 pixels, multi-pixel sampling moiré fringes are generated, and a spatial phase-shifting technique is combined for a full-field deformation measurement. The strain measurement accuracy is significantly improved, and automatic batch measurement is easily achievable. Both methods can measure the two-dimensional (2D) strain distributions from a single-shot grid image without rotating the specimen or scanning lines, as in traditional moiré techniques. As examples, the 2D displacement and strain distributions, including the shear strains of two carbon fiber-reinforced plastic specimens, were measured in three-point bending tests. The proposed technique is expected to play an important role in the non-destructive quantitative evaluations of mechanical properties, crack occurrences, and residual stresses of a variety of materials.

Development of a quick and easy-to-install strain measurement tool for piping stress evaluation

International Nuclear Information System (INIS)

Takahama, Tsunemichi; Nishimura, Kazuma; Ninomiya, Seiichiro; Matsumoto, Yoshihiro; Harada, Yutaka

2015-01-01

To avoid failures of small bore piping connections caused by high cycle fatigue, it is important to measure the stresses around the connections. To measure such stresses, the authors have developed an easily-attachable and detachable strain measurement tool which utilizes strain gauges in combination with our patented strain gauge holder. Traditionally, strain gauges have been bonded to piping surfaces using adhesive; however, with the newly-developed measurement tool, bonding adhesive is no longer necessary. The tool can be installed quickly and easily on a piping surface and measure the strains on the piping as accurately as adhesively-bonded strain gauges. Accordingly, the new strain measurement tool significantly reduces the work time without affecting the measurement accuracy. (author)

Hybrid MEFPI/FBG sensor for simultaneous measurement of strain and magnetic field

Science.gov (United States)

Chen, Mao-qing; Zhao, Yong; Lv, Ri-qing; Xia, Feng

2017-12-01

A hybrid fiber-optic sensor consisting of a micro extrinsic Fabry-Perot Interferometer (MEFPI) and an etched fiber Bragg grating (FBG) is proposed, which can measure strain and magnetic field simultaneously. The etched FBG is sealed in a capillary with ferrofluids to detect the surrounding magnetic field. FBG with small diameter will be more sensitive to magnetic field is confirmed by simulation results. The MEFPI sensor that is prepared through welding a short section of hollow-core fiber (HCF) with single-mode fiber (SMF) is effective for strain detection. The experiment shows that strain and magnetic field can be successfully simultaneously detected based on hybrid MEFPI/FBG sensor. The sensitivities of the strain and magnetic field intensity are measured to be up to 1.41 pm/με and 5.11 pm/mT respectively. There is a negligible effect on each other, hence simultaneously measuring strain and magnetic field is feasible. It is anticipated that such easy preparation, compact and low-cost fiber-optic sensors for simultaneous measurement of strain and magnetic field could find important applications in practice.

Polymer film strain gauges for measuring large elongations

Science.gov (United States)

Kondratov, A. P.; Zueva, A. M.; Varakin, R. S.; Taranec, I. P.; Savenkova, I. A.

2018-02-01

The paper shows the possibility to print polymer strain gages, microstrip lines, coplanar waveguides, and other prints for avionics using printing technology and equipment. The methods of screen and inkjet printing have been complemented by three new operations of preparing print films for application of an electrically conductive ink layer. Such additional operations make it possible to enhance the conductive ink layer adhesion to the film and to manufacture strain gages for measuring large elongations.

Methodology for dynamic biaxial tension testing of pregnant uterine tissue.

Science.gov (United States)

Manoogian, Sarah; Mcnally, Craig; Calloway, Britt; Duma, Stefan

2007-01-01

Placental abruption accounts for 50% to 70% of fetal losses in motor vehicle crashes. Since automobile crashes are the leading cause of traumatic fetal injury mortality in the United States, research of this injury mechanism is important. Before research can adequately evaluate current and future restraint designs, a detailed model of the pregnant uterine tissues is necessary. The purpose of this study is to develop a methodology for testing the pregnant uterus in biaxial tension at a rate normally seen in a motor vehicle crash. Since the majority of previous biaxial work has established methods for quasi-static testing, this paper combines previous research and new methods to develop a custom designed system to strain the tissue at a dynamic rate. Load cells and optical markers are used for calculating stress strain curves of the perpendicular loading axes. Results for this methodology show images of a tissue specimen loaded and a finite verification of the optical strain measurement. The biaxial test system dynamically pulls the tissue to failure with synchronous motion of four tissue grips that are rigidly coupled to the tissue specimen. The test device models in situ loading conditions of the pregnant uterus and overcomes previous limitations of biaxial testing. A non-contact method of measuring strains combined with data reduction to resolve the stresses in two directions provides the information necessary to develop a three dimensional constitutive model of the material. Moreover, future research can apply this method to other soft tissues with similar in situ loading conditions.

Draft Genome Sequence of the Biocontrol Strain Serratia plymuthica A30, Isolated from Rotting Potato Tuber Tissue

NARCIS (Netherlands)

Czajkowski, R.L.; Wolf, van der J.M.

2012-01-01

Serratia plymuthica A30 is a Gram-negative bacterium expressing antagonistic activity toward blackleg- and soft rot-causing Dickeya sp. biovar 3 (“Dickeya solani”). Here, we present the draft genome sequence of strain A30, which has been isolated from rotten potato tuber tissue

Dynamic strain measurement of hydraulic system pipeline using fibre Bragg grating sensors

Directory of Open Access Journals (Sweden)

Qiang Wang

2016-04-01

Full Text Available Fatigue failure is a serious problem in hydraulic piping systems installed in the machinery and equipment working in harsh operational conditions. To alleviate this problem, health monitoring of pipes can be conducted by measuring and analysing vibration-induced strain. Fibre Bragg grating is considered as a promising sensing approach for dynamic load monitoring. In this article, dynamic strain measurements based on fibre Bragg grating sensors for small-bore metal pipes have been investigated. The quasi-distributed strain sensing of fibre Bragg grating sensors is introduced. Two comparison experiments were carried out under vibration and impact loads among the methods of electrical strain gauge, piezoelectric accelerometer and fibre Bragg grating sensor. Experimental results indicate that fibre Bragg grating sensor possesses an outstanding ability to resist electromagnetic interference compared with strain gauge. The natural frequency measurement results, captured by fibre Bragg grating sensor, agree well with the modal analysis results obtained from finite element analysis. In addition, the attached fibre Bragg grating sensor brings a smaller impact on the dynamic characteristics of the measured pipe than the accelerometer due to its small size and lightweight. Fibre Bragg grating sensors have great potential for the quasi-distributed measurement of dynamic strain for the dynamic characteristic research and health monitoring of hydraulic system pipeline.

Measurement of strains by means of electro-optics holography

Science.gov (United States)

Sciammarella, Cesar A.; Bhat, Gopalakrishna K.; Albertazzi, Armando, Jr.

1991-03-01

The use of a TV camera as a recording medium and the observation of whole field displacements in real time makes holographic TV a very interesting and powerful tool in a variety of areas from NDE to research and development. The paper presents new developments in the field that add to the versatility of the technique by introducing portability and methods to obtain accurate quantitative results. Examples of applications are given to the measurement of strains both at room and at high temperatures and strain measurements at the microscopic level. 1.

Technique for measurements of plane waves of uniaxial strain

International Nuclear Information System (INIS)

Graham, R.A.

1977-01-01

The measurement of plane waves in uniaxial strain, in which large surface areas are loaded and the measurements are restricted to a central region that is not influenced by lateral boundaries, is discussed. Measuring techniques are covered and instruments are discussed

Viscoelastic properties of bovine orbital connective tissue and fat: constitutive models.

Science.gov (United States)

Yoo, Lawrence; Gupta, Vijay; Lee, Choongyeop; Kavehpore, Pirouz; Demer, Joseph L

2011-12-01

Reported mechanical properties of orbital connective tissue and fat have been too sparse to model strain-stress relationships underlying biomechanical interactions in strabismus. We performed rheological tests to develop a multi-mode upper convected Maxwell (UCM) model of these tissues under shear loading. From 20 fresh bovine orbits, 30 samples of connective tissue were taken from rectus pulley regions and 30 samples of fatty tissues from the posterior orbit. Additional samples were defatted to determine connective tissue weight proportion, which was verified histologically. Mechanical testing in shear employed a triborheometer to perform: strain sweeps at 0.5-2.0 Hz; shear stress relaxation with 1% strain; viscometry at 0.01-0.5 s(-1) strain rate; and shear oscillation at 1% strain. Average connective tissue weight proportion was 98% for predominantly connective tissue and 76% for fatty tissue. Connective tissue specimens reached a long-term relaxation modulus of 668 Pa after 1,500 s, while corresponding values for fatty tissue specimens were 290 Pa and 1,100 s. Shear stress magnitude for connective tissue exceeded that of fatty tissue by five-fold. Based on these data, we developed a multi-mode UCM model with variable viscosities and time constants, and a damped hyperelastic response that accurately described measured properties of both connective and fatty tissues. Model parameters differed significantly between the two tissues. Viscoelastic properties of predominantly connective orbital tissues under shear loading differ markedly from properties of orbital fat, but both are accurately reflected using UCM models. These viscoelastic models will facilitate realistic global modeling of EOM behavior in binocular alignment and strabismus.

Draft Genome Sequence of the Biocontrol Strain Serratia plymuthica A30, Isolated from Rotting Potato Tuber Tissue

NARCIS (Netherlands)

Czajkowski, R.L.; Van der Wolf, J.M.

2012-01-01

Serratia plymuthica A30 is a Gram-negative bacterium expressing antagonistic activity toward blackleg- and soft rot-causing Dickeya sp. biovar 3 ("Dickeya solani"). Here, we present the draft genome sequence of strain A30, which has been isolated from rotten potato tuber tissue. [KEYWORDS: biovar 3

Stress strain tensors with their application to x-ray stress measurement

International Nuclear Information System (INIS)

Kurita, Masanori

2015-01-01

This paper describes in detail the method of obtaining the formulas of stress-strain tensor that express the directional dependence of stress-strain, that is, how these values change in response to coordinate transformation, and clarifies the preconditions for supporting both formulas. The two conversion formulas are both the second order of tensor, and the formula of strain tensor not only does not use the relational expression of stress and strain at all, but also is obtained completely independently of the formula of stress tensor. Except for the condition that the strain is very small (elastic deformation) in the conversion formula of strain, both formulas unconditionally come into effect. In other words, both formulas hold true even in the isotropic elastic body or anisotropic elastic body. It was shown that the conversion formula of strain can be derived from the conversion formula of stress using the formula of Hooke for isotropic elastic body. From these three-dimensional expressions, the two-dimensional stress-strain coordinate conversion formula that is used for Mohr's stress-strain circle was derived. It was shown that these formulas hold true for three-dimensional stress condition with stress-strain components in the three-axial direction that are not plane stress nor plane strain condition. In addition, as an application case of this theory, two-dimensional and three-dimensional X-ray stress measurements that are effective for residual stress measurement were shown. (A.O.)

Local, atomic-level elastic strain measurements of metallic glass thin films by electron diffraction

Energy Technology Data Exchange (ETDEWEB)

Ebner, C. [Physics of Nanostructured Materials, Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna (Austria); Sarkar, R. [Department of Materials Science and Engineering, School for Engineering of Matter Transport and Energy, Arizona State University, Tempe 85287 (United States); Rajagopalan, J. [Department of Materials Science and Engineering, School for Engineering of Matter Transport and Energy, Arizona State University, Tempe 85287 (United States); Department of Mechanical and Aerospace Engineering, School for Engineering of Matter Transport and Energy, Arizona State University, Tempe 85287 (United States); Rentenberger, C., E-mail: christian.rentenberger@univie.ac.at [Physics of Nanostructured Materials, Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna (Austria)

2016-06-15

A novel technique is used to measure the atomic-level elastic strain tensor of amorphous materials by tracking geometric changes of the first diffuse ring of selected area electron diffraction patterns (SAD). An automatic procedure, which includes locating the centre and fitting an ellipse to the diffuse ring with sub-pixel precision is developed for extracting the 2-dimensional strain tensor from the SAD patterns. Using this technique, atomic-level principal strains from micrometre-sized regions of freestanding amorphous Ti{sub 0.45}Al{sub 0.55} thin films were measured during in-situ TEM tensile deformation. The thin films were deformed using MEMS based testing stages that allow simultaneous measurement of the macroscopic stress and strain. The calculated atomic-level principal strains show a linear dependence on the applied stress, and good correspondence with the measured macroscopic strains. The calculated Poisson’s ratio of 0.23 is reasonable for brittle metallic glasses. The technique yields a strain accuracy of about 1×10{sup −4} and shows the potential to obtain localized strain profiles/maps of amorphous thin film samples. - Highlights: • A TEM method to measure elastic strain in metallic glass films is proposed. • Method is based on tracking geometric changes in TEM diffraction patterns. • An automatic procedure is developed for extracting the local strain tensor. • Atomic-level strain in amorphous TiAl film was analysed during in-situ deformation. • Capability of the method to obtain micrometer scale strain profiles/maps is shown.

Analytical stiffness matrices with Green-Lagrange strain measure

DEFF Research Database (Denmark)

Pedersen, Pauli

2005-01-01

Separating the dependence on material and stress/strain state from the dependence on initial geometry, we obtain analytical secant and tangent stiffness matrices. For the case of a linear displacement triangle with uniform thickness and uniform constitutive behaviour closed-form results are listed...... a solution based on Green-Lagrange strain measure. The approach is especially useful in design optimization, because analytical sensitivity analysis then can be performed. The case of a three node triangular ring element for axisymmetric analysis involves small modifications and extension to four node...

Measurement of in-plane strain with dual beam spatial phase-shift digital shearography

International Nuclear Information System (INIS)

Xie, Xin; Chen, Xu; Li, Junrui; Yang, Lianxiang; Wang, Yonghong

2015-01-01

Full-field in-plane strain measurement under dynamic loading by digital shearography remains a big challenge in practice. A phase measurement for in-plane strain information within one time frame has to be achieved to solve this problem. This paper presents a dual beam spatial phase-shift digital shearography system with the capacity to measure phase distribution corresponding to in-plane strain information within a single time frame. Two laser beams with different wavelengths are symmetrically arranged to illuminate the object under test, and two cameras with corresponding filters, which enable simultaneous recording of two shearograms, are utilized for data acquisition. The phase information from the recorded shearograms, which corresponds to the in-plane strain, is evaluated by the spatial phase-shift method. The spatial phase-shift shearography system realizes a measurement of the in-plane strain through the introduction of the spatial phase-shift technique, using one frame after the loading and one frame before loading. This paper presents the theory of the spatial phase-shift digital shearography for in-plane strain measurement and its derivation, experimental results, and the technique’s potential. (paper)

Application of a fiber optic grating strain sensor for the measurement of strain under irradiation environment

International Nuclear Information System (INIS)

Kaji, Yoshiyuki; Matsui, Yoshinori; Kita, Satoshi; Ide, Hiroshi; Tsukada, Takashi; Tsuji, Hirokazu

2002-01-01

In the Japan Atomic Energy Research Institute (JAERI), in-pile strain measurement techniques have been developed using the Japan Materials Testing Reactor (JMTR). In order to evaluate the performance of fiber optic grating sensors under irradiation environment, heat-up and performance tests at elevated temperatures before irradiation and in-pile tests were performed in JMTR. It was determined that it is possible to measure strain under irradiation environment below 1x10 23 n m -2 (E>1 MeV) by a fiber optic grating sensor, because in-pile temperature characteristics were in good agreement with out-of-pile test results

WE-EF-210-06: Ultrasound 2D Strain Measurement of Radiation-Induced Toxicity: Phantom and Ex Vivo Experiments

Energy Technology Data Exchange (ETDEWEB)

Liu, T; Torres, M; Rossi, P; Jani, A; Curran, W; Yang, X [Emory Univ, Atlanta, GA (United States)

2015-06-15

Purpose: Radiation-induced fibrosis is a common long-term complication affecting many patients following cancer radiotherapy. Standard clinical assessment of subcutaneous fibrosis is subjective and often limited to visual inspection and palpation. Ultrasound strain imaging describes the compressibility (elasticity) of biological tissues. This study’s purpose is to develop a quantitative ultrasound strain imaging that can consistently and accurately characterize radiation-induce fibrosis. Methods: In this study, we propose a 2D strain imaging method based on deformable image registration. A combined affine and B-spline transformation model is used to calculate the displacement of tissue between pre-stress and post-stress B-mode image sequences. The 2D displacement is estimated through a hybrid image similarity measure metric, which is a combination of the normalized mutual information (NMI) and normalized sum-of-squared-differences (NSSD). And 2D strain is obtained from the gradient of the local displacement. We conducted phantom experiments under various compressions and compared the performance of our proposed method with the standard cross-correlation (CC)- based method using the signal-to-noise (SNR) and contrast-to-noise (CNS) ratios. In addition, we conducted ex-vivo beef muscle experiment to further validate the proposed method. Results: For phantom study, the SNR and CNS values of the proposed method were significantly higher than those calculated from the CC-based method under different strains. The SNR and CNR increased by a factor of 1.9 and 2.7 comparing to the CC-based method. For the ex-vivo experiment, the CC-based method failed to work due to large deformation (6.7%), while our proposed method could accurately detect the stiffness change. Conclusion: We have developed a 2D strain imaging technique based on the deformable image registration, validated its accuracy and feasibility with phantom and ex-vivo data. This 2D ultrasound strain imaging

The mechanical heterogeneity of the hard callus influences local tissue strains during bone healing: a finite element study based on sheep experiments.

Science.gov (United States)

Vetter, A; Liu, Y; Witt, F; Manjubala, I; Sander, O; Epari, D R; Fratzl, P; Duda, G N; Weinkamer, R

2011-02-03

During secondary fracture healing, various tissue types including new bone are formed. The local mechanical strains play an important role in tissue proliferation and differentiation. To further our mechanobiological understanding of fracture healing, a precise assessment of local strains is mandatory. Until now, static analyses using Finite Elements (FE) have assumed homogenous material properties. With the recent quantification of both the spatial tissue patterns (Vetter et al., 2010) and the development of elastic modulus of newly formed bone during healing (Manjubala et al., 2009), it is now possible to incorporate this heterogeneity. Therefore, the aim of this study is to investigate the effect of this heterogeneity on the strain patterns at six successive healing stages. The input data of the present work stemmed from a comprehensive cross-sectional study of sheep with a tibial osteotomy (Epari et al., 2006). In our FE model, each element containing bone was described by a bulk elastic modulus, which depended on both the local area fraction and the local elastic modulus of the bone material. The obtained strains were compared with the results of hypothetical FE models assuming homogeneous material properties. The differences in the spatial distributions of the strains between the heterogeneous and homogeneous FE models were interpreted using a current mechanobiological theory (Isakson et al., 2006). This interpretation showed that considering the heterogeneity of the hard callus is most important at the intermediate stages of healing, when cartilage transforms to bone via endochondral ossification. Copyright © 2010 Elsevier Ltd. All rights reserved.

Photonic Crystal Fiber Sensors for Strain and Temperature Measurement

Directory of Open Access Journals (Sweden)

Jian Ju

2009-01-01

Full Text Available This paper discusses the applications of photonic crystal fibers (PCFs for strain and temperature measurement. Long-period grating sensors and in-fiber modal interferometric sensors are described and compared with their conventional single-mode counterparts. The strain sensitivities of the air-silica PCF sensors are comparable or higher than those implemented in conventional single-mode fibers but the temperature sensitivities of the PCF sensors are much lower.

Modeling of stress-strain diagram on the basis of magnetic measurements

International Nuclear Information System (INIS)

Gorkunov, Eh.S.; Fedotov, V.P.; Bukhvalov, A.B.; Veselov, I.N.

1997-01-01

The model of a stress-strain diagram with taking into account the strain hardening and the growth of metal damageing is proposed. The model is applied to calculate a stress-strain curve for continuous cast 45 steel using the results of magnetic properties measuring. The latter permits predicting the durability of construction elements with the use of nondestructive magnetic testing

Brain tissue strains vary with head impact location: A possible explanation for increased concussion risk in struck versus striking football players.

Science.gov (United States)

Elkin, Benjamin S; Gabler, Lee F; Panzer, Matthew B; Siegmund, Gunter P

2018-03-29

On-field football helmet impacts over a large range of severities have caused concussions in some players but not in other players. One possible explanation for this variability is the struck player's helmet impact location. We examined the effect of impact location on regional brain tissue strain when input energy was held constant. Laboratory impacts were performed at 12 locations distributed over the helmet and the resulting head kinematics were simulated in two finite element models of the brain: the Simulated Injury Monitor and the Global Human Body Model Consortium brain model. Peak kinematics, injury metrics and brain strain varied significantly with impact location. Differences in impact location explained 33 to 37% of the total variance in brain strain for the whole brain and cerebrum, considerably more than the variance explained by impact location for the peak resultant head kinematics (8 to 23%) and slightly more than half of the variance explained by the difference in closing speed (57 to 61%). Both finite element models generated similar strain results, with minor variations for impacts that generated multi-axial rotations, larger variations in brainstem strains for some impact locations and a small bias for the cerebellum. Based on this experimental and computational simulation study, impact location on the football helmet has a large effect on regional brain tissue strain. We also found that the lowest strains consistently occurred in impacts to the crown and forehead, helmet locations commonly associated with the striking player. Copyright © 2018 Elsevier Ltd. All rights reserved.

A fully implantable telemetry system for the long-term measurement of habitual bone strain

NARCIS (Netherlands)

de Jong, W. C.; Koolstra, J. H.; van Ruijven, L. J.; Korfage, J. A. M.; Langenbach, G. E. J.

2010-01-01

Long-term in-vivo recordings of habitual bone strain in freely moving animals are needed to better understand the everyday mechanical loading environment responsible for bone-tissue maintenance. However, wireless methods to make such recordings are scarce. We report on the successful customisation

Measurement of strains at high temperatures by means of electro-optics holography

Science.gov (United States)

Sciammarella, Cesar A.; Bhat, G.; Vaitekunas, Jeffrey

Electro-optics holographic-moire interferometry is used to measure strains at temperatures up to 1000 C. A description of the instrumentation developed to carry out the measurements is given. The data processing technique is also explained. Main problems encountered in recording patterns at high temperatures are analyzed and possible solutions are outlined. Optical results are compared with strain gage values obtained with instrumented specimens and with theoretical results. Very good agreement is found between optical, strain gage and theoretical results.

A novel compliance measurement in radial arteries using strain-gauge plethysmography

International Nuclear Information System (INIS)

Liu, Shing-Hong; Tyan, Chu-Chang; Chang, Kang-Ming

2009-01-01

We propose a novel method for assessing the compliance of the radial artery by using a two-axis mechanism and a standard positioning procedure for detecting the optimal measuring site. A modified sensor was designed to simultaneously measure the arterial diameter change waveform (ADCW) and pressure pulse waveform with a strain gauge and piezoresistor. In the x-axis scanning, the sensor could be placed close to the middle of the radial artery when the ADCW reached the maximum amplitude. In the z-axis scanning, the contact pressure was continuously increased for data measurement. Upon the deformation of the strain gauge following the change in the vascular cross-section, the ADCW was transferred to the change of the vascular radius. The loaded strain compliance of the radial artery (C strain ) can be determined by dividing the dynamic changed radius by the pulse pressure. Twenty-three untreated, mild or moderate hypertensive patients aged 29–85 were compared with 14 normotensive patients aged 25–62. The maximum strain compliance between the two groups was significantly different (p < 0.005). Of the hypertensive patients, 14 were at risk of developing hyperlipidemia. There was a significant difference between this and the normotension group (p < 0.005)

A novel compliance measurement in radial arteries using strain-gauge plethysmography.

Science.gov (United States)

Liu, Shing-Hong; Tyan, Chu-Chang; Chang, Kang-Ming

2009-09-01

We propose a novel method for assessing the compliance of the radial artery by using a two-axis mechanism and a standard positioning procedure for detecting the optimal measuring site. A modified sensor was designed to simultaneously measure the arterial diameter change waveform (ADCW) and pressure pulse waveform with a strain gauge and piezoresistor. In the x-axis scanning, the sensor could be placed close to the middle of the radial artery when the ADCW reached the maximum amplitude. In the Z-axis scanning, the contact pressure was continuously increased for data measurement. Upon the deformation of the strain gauge following the change in the vascular cross-section, the ADCW was transferred to the change of the vascular radius. The loaded strain compliance of the radial artery (C(strain)) can be determined by dividing the dynamic changed radius by the pulse pressure. Twenty-three untreated, mild or moderate hypertensive patients aged 29-85 were compared with 14 normotensive patients aged 25-62. The maximum strain compliance between the two groups was significantly different (p < 0.005). Of the hypertensive patients, 14 were at risk of developing hyperlipidemia. There was a significant difference between this and the normotension group (p < 0.005).

Fabrication and evaluation of hybrid silica/polymer optical fiber sensors for large strain measurement

Science.gov (United States)

Huang, Haiying

2007-04-01

Silica-based optical fiber sensors are widely used in structural health monitoring systems for strain and deflection measurement. One drawback of silica-based optical fiber sensors is their low strain toughness. In general, silica-based optical fiber sensors can only reliably measure strains up to 2%. Recently, polymer optical fiber sensors have been employed to measure large strain and deflection. Due to their high optical losses, the length of the polymer optical fibers is limited to 100 meters. In this paper, we present a novel economical technique to fabricate hybrid silica/polymer optical fiber strain sensors for large strain measurement. First, stress analysis of a surface-mounted optical fiber sensor is performed to understand the load distribution between the host structure and the optical fiber in relation to their mechanical properties. Next, the procedure of fabricating a polymer sensing element between two optical fibers is explained. The experimental set-up and the components used in the fabrication process are described in details. Mechanical testing results of the fabricated silica/polymer optical fiber strain sensor are presented.

Application of computer picture processing to dynamic strain measurement under electromagnetic field

International Nuclear Information System (INIS)

Yagawa, G.; Soneda, N.

1987-01-01

For the structural design of fusion reactors, it is very important to ensure the structural integrity of components under various dynamic loading conditions due to a solid-electromagnetic field interaction, an earthquake, MHD effects and so on. As one of the experimental approaches to assess the dynamic fracture, we consider the strain measurement near a crack tip under a transient electromagnetic field, which in general involves several experimental difficulties. The authors have developed a strain measurement method using a picture processing technique. In this method, locations of marks printed on a surface of specimen are determined by the picture processing. The displacement field is interpolated using the mark displacements and finite elements. Finally the strain distribution is calculated by differentiating the displacement field. In the present study, the method is improved and automated apply to the measurement of dynamic strain distribution under an electromagnetic field. Then the effects of dynamic loading on the strain distribution are investigated by comparing the dynamic results with the static ones. (orig./GL)

Passive wireless strain measurement based upon the Villari effect and giant magnetoresistance

Science.gov (United States)

Windl, Roman; Bruckner, Florian; Abert, Claas; Huber, Christian; Vogler, Christoph; Huber, Thomas; Oezelt, Harald; Suess, Dieter

2016-12-01

A passive wireless radio frequency-identification (RFID) stress/strain sensor is presented. Stress is transformed into a change of magnetic field by utilizing an amorphous metal ribbon. This magnetic field change is measured by a giant magnetoresistance magnetic field sensor and converted into a digital value with a RFID chip for wireless access. Standard metal foil strain gauges have a gauge factor GF from around 2 to 5 and suffer from the disadvantage of a physically connected power supply and measurement equipment. For the presented sensor, a strain range of -10 μm/m to 190 μm/m results in a linear sensor response, a gauge factor of GF ≈ 245, and a detectivity of 4.10 nm/m 1/√{Hz } . The detectivity of the presented sensor is similar to the detectivity of a reference metal foil strain gauge. Due to low power consumption and easy signal analysis, this sensor is well suited for long term strain measurement inside closed spaces. RFID adds features like multiple tag detection, wireless passive operation and a user data storage.

Reduced thoracolumbar fascia shear strain in human chronic low back pain

Directory of Open Access Journals (Sweden)

Konofagou Elisa E

2011-09-01

Full Text Available Abstract Background The role played by the thoracolumbar fascia in chronic low back pain (LBP is poorly understood. The thoracolumbar fascia is composed of dense connective tissue layers separated by layers of loose connective tissue that normally allow the dense layers to glide past one another during trunk motion. The goal of this study was to quantify shear plane motion within the thoracolumbar fascia using ultrasound elasticity imaging in human subjects with and without chronic low back pain (LBP. Methods We tested 121 human subjects, 50 without LBP and 71 with LBP of greater than 12 months duration. In each subject, an ultrasound cine-recording was acquired on the right and left sides of the back during passive trunk flexion using a motorized articulated table with the hinge point of the table at L4-5 and the ultrasound probe located longitudinally 2 cm lateral to the midline at the level of the L2-3 interspace. Tissue displacement within the thoracolumbar fascia was calculated using cross correlation techniques and shear strain was derived from this displacement data. Additional measures included standard range of motion and physical performance evaluations as well as ultrasound measurement of perimuscular connective tissue thickness and echogenicity. Results Thoracolumbar fascia shear strain was reduced in the LBP group compared with the No-LBP group (56.4% ± 3.1% vs. 70.2% ± 3.6% respectively, p Conclusion Thoracolumbar fascia shear strain was ~20% lower in human subjects with chronic low back pain. This reduction of shear plane motion may be due to abnormal trunk movement patterns and/or intrinsic connective tissue pathology. There appears to be some sex-related differences in thoracolumbar fascia shear strain that may also play a role in altered connective tissue function.

Enhanced Strain Measurement Range of an FBG Sensor Embedded in Seven-Wire Steel Strands.

Science.gov (United States)

Kim, Jae-Min; Kim, Chul-Min; Choi, Song-Yi; Lee, Bang Yeon

2017-07-18

FBG sensors offer many advantages, such as a lack of sensitivity to electromagnetic waves, small size, high durability, and high sensitivity. However, their maximum strain measurement range is lower than the yield strain range (about 1.0%) of steel strands when embedded in steel strands. This study proposes a new FBG sensing technique in which an FBG sensor is recoated with polyimide and protected by a polyimide tube in an effort to enhance the maximum strain measurement range of FBG sensors embedded in strands. The validation test results showed that the proposed FBG sensing technique has a maximum strain measurement range of 1.73% on average, which is 1.73 times higher than the yield strain of the strands. It was confirmed that recoating the FBG sensor with polyimide and protecting the FBG sensor using a polyimide tube could effectively enhance the maximum strain measurement range of FBG sensors embedded in strands.

Strain sensing systems tailored for tensile measurement of fragile wires

Science.gov (United States)

Nyilas, Arman

2005-12-01

Fundamental stress versus strain measurements were completed on superconducting Nb3Sn wires within the framework of IEC/TC90 and VAMAS/TWA16. A key task was the assessment of sensing systems regarding resolution, accuracy, and precision when measuring Young's modulus. Prior to actual Nb3Sn wire measurements metallic wires, consisting of copper and stainless steel having diameters similar to the Nb3Sn wire, were extensively investigated with respect to their elastic line properties using different types of extensometers. After these calibration tests Nb3Sn wire measurements of different companies resulted in several important facts with respect to total size and weight of the used extensometers. The size could be correlated to the initial stage of stress versus strain behaviour. In fact, the effect of wire curls resulting from the production line had a profound effect on Young's modulus measurements. Within this context, the possibility of determining Young's modulus from unloading compliance lines in the plastic regime of the stress-strain curve was considered. The data obtained using this test methodology were discussed under consideration of the composite nature of Nb3Sn wire. In addition, a non-contacting sensing system based on a double-beam laser extensometer was used to investigate the potential of this new sensing system.

Strain sensing systems tailored for tensile measurement of fragile wires

International Nuclear Information System (INIS)

Nyilas, Arman

2005-01-01

Fundamental stress versus strain measurements were completed on superconducting Nb 3 Sn wires within the framework of IEC/TC90 and VAMAS/TWA16. A key task was the assessment of sensing systems regarding resolution, accuracy, and precision when measuring Young's modulus. Prior to actual Nb 3 Sn wire measurements metallic wires, consisting of copper and stainless steel having diameters similar to the Nb 3 Sn wire, were extensively investigated with respect to their elastic line properties using different types of extensometers. After these calibration tests Nb 3 Sn wire measurements of different companies resulted in several important facts with respect to total size and weight of the used extensometers. The size could be correlated to the initial stage of stress versus strain behaviour. In fact, the effect of wire curls resulting from the production line had a profound effect on Young's modulus measurements. Within this context, the possibility of determining Young's modulus from unloading compliance lines in the plastic regime of the stress-strain curve was considered. The data obtained using this test methodology were discussed under consideration of the composite nature of Nb 3 Sn wire. In addition, a non-contacting sensing system based on a double-beam laser extensometer was used to investigate the potential of this new sensing system

Interpretation of strain measurements on nuclear pressure vessels

International Nuclear Information System (INIS)

Andersen, S.I.; Engbaek, P.

1979-11-01

Selected results from strain measurements on 4 nuclear pressure vessels are presented and discussed. The measurements were made in several different regions of the vessels: transition zones in vessel heads, flanges and bottom parts, nozzels, internal vessel structure and flange bolts. The results presented are based on data obtained by approximately 700 strain-gauges, and a comprehensive knowledge of the quality obtained by such measurements is established. It is shown that a thorough control procedure before and after the test as well as detailed knowledge of the behaviour of the signal from the individual gauges during the test is necessary. If this is omitted, it can be extremely difficult to distinguish between the real structural behaviour and a malfunctioning of a specific gauge installation. In general, most of the measuring results exhibit a very linear behaviour with a negligible zeroshift. However, deviations from linear behaviour are observed in several cases. This nonlinearity can be explained by friction (flange connections) or by gaps (concentrical nozzles) in certain regions, whereas local plastic deformations during the first pressure loadings of the vessel seem to be the reason in other regions. (author)

Reliability Assessment for PSC Box-Girder Bridges Based on SHM Strain Measurements

Directory of Open Access Journals (Sweden)

Chuang Chen

2017-01-01

Full Text Available A reliability assessment method for prestressed concrete (PSC continuous box-girder bridges based on structural health monitoring (SHM strain measurements was proposed. First, due to the fact that measured strain was compositive and the variation periods of its components were different, a series of limit state equations under normal use limit state were given. Then, a linear fitting method was used to determine the relationship between the ambient temperature and the measured strain, which was aimed at extracting the vehicle load effect and the temperature load effect from the measured strain. Finally, according to the equivalent normalization method, the load effects unsatisfying the normal distribution by probability density function fitting were transformed, and the daily failure probabilities of monitored positions were calculated for evaluating the safety state of the girder. The results show that (1 the top plate of the box girder is more sensitive than the bottom plate to the high temperature, (2 the daily and seasonal strain variations induced by uniform temperature reveal an inconsistent tendency to the seasonal variation for mid-span cross sections, and (3 the generalized extreme value distribution is recommended for temperature gradient stress and vehicle induced stress fitting for box-girder bridges.

Concordance and reproducibility between M-mode, tissue Doppler imaging, and two-dimensional strain imaging in the assessment of mitral annular displacement and velocity in patients with various heart conditions

DEFF Research Database (Denmark)

de Knegt, Martina Chantal; Biering-Sorensen, Tor; Sogaard, Peter

2014-01-01

AIMS: Mitral annular (MA) displacement reflects longitudinal left ventricular (LV) deformation and systolic velocity measurements reflect the rate of contraction; both are valuable in the diagnosis and prognosis of cardiac disease. The aim of this study was to test the agreement and reproducibility...... between motion mode (M-mode), colour tissue Doppler imaging (TDI), and two-dimensional strain imaging (2DSI) when measuring MA displacement and systolic velocity. METHODS AND RESULTS: Using GE Healthcare Vivid 7 and E9 and Echopac BT11 software, MA displacement and velocity measurements by 2DSI, TDI...

Measurement test on creep strain rate of uranium-zirconium solid solutions

International Nuclear Information System (INIS)

Ogata, Takanari; Akabori, Mitsuo; Ogawa, Toru

1996-11-01

In order to measure creep strain rate of a small specimen of U-Zr solid solution, authors proposed an estimation method which was based upon the stress relaxation after compression. It was applied to measurement test on creep strain rate of the U-10wt%Zr specimen in the temperature range of 757 to 911degC. It may be concluded that the proposed method is valid, provided that the strain is within the appropriate range and that sufficient amount of the load decrement is observed. The obtained creep rate of U-10wt%Zr alloy indicated significantly smaller value, compared to the experimental data for pure U metal and evaluated data for U-Pu-Zr alloy. However, more careful measurement is desired in future since the present data are thought to be influenced by the precipitations included in the specimen. (author)

X-Ray-Scattering Measurements Of Strain In PEEK

Science.gov (United States)

Cebe, Peggy; Lowry, Lynn E.; Chung, Shirley Y.; Yavrouian, Andre H.; Gupta, Amitava

1988-01-01

Internal stress relieved by heating above glass-transition temperature. Report describes wide-angle x-ray scattering and differential scanning calorimetry of specimens of poly(etheretherketone) having undergone various thermal treatments. Wide-angle x-ray scattering particularly useful in determining distances between atoms, crystallinity, and related microstructurally generated phenomena, as thermal expansion and strain. Calorimetric measurements aid interpretation of scattering measurements by enabling correlation with thermal effects.

Distributed strain measurement in perfluorinated polymer optical fibres using optical frequency domain reflectometry

International Nuclear Information System (INIS)

Liehr, Sascha; Wendt, Mario; Krebber, Katerina

2010-01-01

We present the latest advances in distributed strain measurement in perfluorinated polymer optical fibres (POFs) using backscatter techniques. Compared to previously introduced poly(methyl methacrylate) POFs, the measurement length can be extended to more than 500 m at improved spatial resolution of a few centimetres. It is shown that strain in a perfluorinated POF can be measured up to 100%. In parallel to these investigations, the incoherent optical frequency domain reflectometry (OFDR) technique is introduced to detect strained fibre sections and to measure distributed length change along the fibre with sub-millimetre resolution by applying a cross-correlation algorithm to the backscatter signal. The overall superior performance of the OFDR technique compared to the optical time domain reflectometry in terms of accuracy, dynamic range, spatial resolution and measurement speed is presented. The proposed sensor system is a promising technique for use in structural health monitoring applications where the precise detection of high strain is required

Measuring techniques for autogenous strain of cement paste

DEFF Research Database (Denmark)

Lura, Pietro; Jensen, Ole Mejlhede

2006-01-01

of the volumetric method. Water absorption is driven by a lowering of the water activity in the cement paste due to dissolved salts in the pore fluid and to self-desiccation. From the moment of casting, significant water uptake was registered in all experiments. This water uptake influenced the volumetric...... measurements by the same order of magnitude as the autogenous strain itself. By performing the measurements in a paraffin oil bath instead of a water bath, this artefact was eliminated. Furthermore, volumetric measurements performed in paraffin oil gave almost identical results as linear measurements performed...

Novel method for measuring a dense 3D strain map of robotic flapping wings

Science.gov (United States)

Li, Beiwen; Zhang, Song

2018-04-01

Measuring dense 3D strain maps of the inextensible membranous flapping wings of robots is of vital importance to the field of bio-inspired engineering. Conventional high-speed 3D videography methods typically reconstruct the wing geometries through measuring sparse points with fiducial markers, and thus cannot obtain the full-field mechanics of the wings in detail. In this research, we propose a novel system to measure a dense strain map of inextensible membranous flapping wings by developing a superfast 3D imaging system and a computational framework for strain analysis. Specifically, first we developed a 5000 Hz 3D imaging system based on the digital fringe projection technique using the defocused binary patterns to precisely measure the dynamic 3D geometries of rapidly flapping wings. Then, we developed a geometry-based algorithm to perform point tracking on the precisely measured 3D surface data. Finally, we developed a dense strain computational method using the Kirchhoff-Love shell theory. Experiments demonstrate that our method can effectively perform point tracking and measure a highly dense strain map of the wings without many fiducial markers.

Facial Soft Tissue Measurement in Microgravity-induces Fluid Shifts

Science.gov (United States)

Marshburn, Thomas; Cole, Richard; Pavela, James; Garcia, Kathleen; Sargsyan, Ashot

2014-01-01

Fluid shifts are a well-known phenomenon in microgravity, and one result is facial edema. Objective measurement of tissue thickness in a standardized location could provide a correlate with the severity of the fluid shift. Previous studies of forehead tissue thickness (TTf) suggest that when exposed to environments that cause fluid shifts, including hypergravity, head-down tilt, and high-altitude/lowpressure, TTf changes in a consistent and measurable fashion. However, the technique in past studies is not well described or standardized. The International Space Station (ISS) houses an ultrasound (US) system capable of accurate sub-millimeter measurements of TTf. We undertook to measure TTf during long-duration space flight using a new accurate, repeatable and transferable technique. Methods: In-flight and post-flight B-mode ultrasound images of a single astronaut's facial soft tissues were obtained using a Vivid-q US system with a 12L-RS high-frequency linear array probe (General Electric, USA). Strictly mid-sagittal images were obtained involving the lower frontal bone, the nasofrontal angle, and the osseo-cartilaginous junction below. Single images were chosen for comparison that contained identical views of the bony landmarks and identical acoustical interface between the probe and skin. Using Gingko CADx DICOM viewing software, soft tissue thickness was measured at a right angle to the most prominent point of the inferior frontal bone to the epidermis. Four independent thickness measurements were made. Conclusions: Forehead tissue thickness measurement by ultrasound in microgravity is feasible, and our data suggest a decrease in tissue thickness upon return from microgravity environment, which is likely related to the cessation of fluid shifts. Further study is warranted to standardize the technique with regard to the individual variability of the local anatomy in this area.

Brachytherapy dose measurements in heterogeneous tissues

International Nuclear Information System (INIS)

Paiva F, G.; Luvizotto, J.; Salles C, T.; Guimaraes A, P. C.; Dalledone S, P. de T.; Yoriyaz, H.; Rubo, R.

2014-08-01

Recently, Beau lieu et al. published an article providing guidance for Model-Based Dose Calculation Algorithms (MBDCAs), where tissue heterogeneity considerations are addressed. It is well-known that T G-43 formalism which considers only water medium is limited and significant dose differences have been found comparing both methodologies. The aim of the present work is to experimentally quantify dose values in heterogeneous medium using different dose measurement methods and techniques and compare them with those obtained with Monte Carlo simulations. Experiments have been performed using a Nucletron micro Selectron-Hdr Ir-192 brachytherapy source and a heterogeneous phantom composed by PMMA and different tissue equivalent cylinders like bone, lungs and muscle. Several dose measurements were obtained using tissue equivalent materials with height 1.8 cm and 4.3 cm positioned between the radiation source and the detectors. Radiochromic films, TLDs and MOSFET S have been used for the dose measurements. Film dosimetry has been performed using two methodologies: a) linearization for dose-response curve based on calibration curves to create a functional form that linearize s the dose response and b) 177 multichannel analysis dosimetry where the multiple color channels are analyzed allowing to address not only disturbances in the measurements caused by thickness variation in the film layer, but also, separate other external influences in the film response. All experiments have been simulated using the MCNP5 Monte Carlo radiation transport code. Comparison of experimental results are in good agreement with calculated dose values with differences less than 6% for almost all cases. (Author)

Brachytherapy dose measurements in heterogeneous tissues

Energy Technology Data Exchange (ETDEWEB)

Paiva F, G.; Luvizotto, J.; Salles C, T.; Guimaraes A, P. C.; Dalledone S, P. de T.; Yoriyaz, H. [Instituto de Pesquisas Energeticas e Nucleares / CNEN, Av. Lineu Prestes 2242, Cidade Universitaria, 05508-000 Sao Paulo (Brazil); Rubo, R., E-mail: gabrielpaivafonseca@gmail.com [Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, 05403-900 Sao Paulo (Brazil)

2014-08-15

Recently, Beau lieu et al. published an article providing guidance for Model-Based Dose Calculation Algorithms (MBDCAs), where tissue heterogeneity considerations are addressed. It is well-known that T G-43 formalism which considers only water medium is limited and significant dose differences have been found comparing both methodologies. The aim of the present work is to experimentally quantify dose values in heterogeneous medium using different dose measurement methods and techniques and compare them with those obtained with Monte Carlo simulations. Experiments have been performed using a Nucletron micro Selectron-Hdr Ir-192 brachytherapy source and a heterogeneous phantom composed by PMMA and different tissue equivalent cylinders like bone, lungs and muscle. Several dose measurements were obtained using tissue equivalent materials with height 1.8 cm and 4.3 cm positioned between the radiation source and the detectors. Radiochromic films, TLDs and MOSFET S have been used for the dose measurements. Film dosimetry has been performed using two methodologies: a) linearization for dose-response curve based on calibration curves to create a functional form that linearize s the dose response and b) 177 multichannel analysis dosimetry where the multiple color channels are analyzed allowing to address not only disturbances in the measurements caused by thickness variation in the film layer, but also, separate other external influences in the film response. All experiments have been simulated using the MCNP5 Monte Carlo radiation transport code. Comparison of experimental results are in good agreement with calculated dose values with differences less than 6% for almost all cases. (Author)

Inverse calculation of strain profiles from ETDR measurements using artificial neural networks

Directory of Open Access Journals (Sweden)

R. Höhne

2017-12-01

Full Text Available A novel carbon fibre sensor is developed for the spatially resolved strain measurement. A unique feature of the sensor is the fibre-break resistive measurement principle and the two-core transmission line design. The electrical time domain reflectometry (ETDR is used in order to realize a spatially resolved measurement of the electrical parameters of the sensor. In this contribution, the process of mapping between the ETDR signals to the existing strain profile is described. Artificial neural networks (ANNs are used to solve the inverse electromagnetic problem. The investigations were carried out with a sensor patch in a cantilever arm configuration. Overall, 136 experiments with varying strain distribution over the sensor length were performed to generate the necessary training data to learn the ANN model. The validation of the ANN highlights the feasibility as well as the current limits concerning the quantitative accuracy of mapping ETDR signals to strain profiles.

Determination of a tissue-level failure evaluation standard for rat femoral cortical bone utilizing a hybrid computational-experimental method.

Science.gov (United States)

Fan, Ruoxun; Liu, Jie; Jia, Zhengbin; Deng, Ying; Liu, Jun

2018-01-01

Macro-level failure in bone structure could be diagnosed by pain or physical examination. However, diagnosing tissue-level failure in a timely manner is challenging due to the difficulty in observing the interior mechanical environment of bone tissue. Because most fractures begin with tissue-level failure in bone tissue caused by continually applied loading, people attempt to monitor the tissue-level failure of bone and provide corresponding measures to prevent fracture. Many tissue-level mechanical parameters of bone could be predicted or measured; however, the value of the parameter may vary among different specimens belonging to a kind of bone structure even at the same age and anatomical site. These variations cause difficulty in representing tissue-level bone failure. Therefore, determining an appropriate tissue-level failure evaluation standard is necessary to represent tissue-level bone failure. In this study, the yield and failure processes of rat femoral cortical bones were primarily simulated through a hybrid computational-experimental method. Subsequently, the tissue-level strains and the ratio between tissue-level failure and yield strains in cortical bones were predicted. The results indicated that certain differences existed in tissue-level strains; however, slight variations in the ratio were observed among different cortical bones. Therefore, the ratio between tissue-level failure and yield strains for a kind of bone structure could be determined. This ratio may then be regarded as an appropriate tissue-level failure evaluation standard to represent the mechanical status of bone tissue.

Estimation of piping temperature fluctuations based on external strain measurements

International Nuclear Information System (INIS)

Morilhat, P.; Maye, J.P.

1993-01-01

Due to the difficulty to carry out measurements at the inner sides of nuclear reactor piping subjected to thermal transients, temperature and stress variations in the pipe walls are estimated by means of external thermocouples and strain-gauges. This inverse problem is solved by spectral analysis. Since the wall harmonic transfer function (response to a harmonic load) is known, the inner side signal will be obtained by convolution of the inverse transfer function of the system and of the strain measurement enables detection of internal temperature fluctuations in a frequency range beyond the scope of the thermocouples. (authors). 5 figs., 3 refs

Vaccine-induced rabies case in a cow (Bos taurus): Molecular characterisation of vaccine strain in brain tissue.

Science.gov (United States)

Vuta, Vlad; Picard-Meyer, Evelyne; Robardet, Emmanuelle; Barboi, Gheorghe; Motiu, Razvan; Barbuceanu, Florica; Vlagioiu, Constantin; Cliquet, Florence

2016-09-22

Rabies is a fatal neuropathogenic zoonosis caused by the rabies virus of the Lyssavirus genus, Rhabdoviridae family. The oral vaccination of foxes - the main reservoir of rabies in Europe - using a live attenuated rabies virus vaccine was successfully conducted in many Western European countries. In July 2015, a rabies vaccine strain was isolated from the brain tissues of a clinically suspect cow (Bos taurus) in Romania. The nucleotide analysis of both N and G gene sequences showed 100% identity between the rabid animal, the GenBank reference SAD B19 strain and five rabies vaccine batches used for the national oral vaccination campaign targeting foxes. Copyright © 2016 Elsevier Ltd. All rights reserved.

Comparison of shear wave velocity measurements assessed with two different ultrasound systems in an ex-vivo tendon strain phantom.

Science.gov (United States)

Rosskopf, Andrea B; Bachmann, Elias; Snedeker, Jess G; Pfirrmann, Christian W A; Buck, Florian M

2016-11-01

The purpose of this study is to compare the reliability of SW velocity measurements of two different ultrasound systems and their correlation with the tangent traction modulus in a non-static tendon strain model. A bovine tendon was fixed in a custom-made stretching device. Force was applied increasing from 0 up to 18 Newton. During each strain state the tangent traction modulus was determined by the stretcher device, and SW velocity (m/s) measurements using a Siemens S3000 and a Supersonic Aixplorer US machine were done for shear modulus (kPa) calculation. A strong significant positive correlation was found between SW velocity assessed by the two ultrasound systems and the tangent traction modulus (r = 0.827-0.954, p Aixplorer 0.25 ± 0.3 m/s (p = 0.034). Mean difference of SW velocity between the two US-systems was 0.37 ± 0.3 m/s (p = 0.012). In conclusion, SW velocities are highly dependent on mechanical forces in the tendon tissue, but for controlled mechanical loads appear to yield reproducible and comparable measurements using different US systems.

Strain measurement in and analysis for hydraulic test of CPR1000 reactor pressure vessel

International Nuclear Information System (INIS)

Zhou Dan; Zhuang Dongzhen

2013-01-01

The strain measurement in hydraulic test of CPR1000 reactor pressure vessel performed in Dongfang Heavy Machinery Co., Ltd. is introduced. The detail test scheme and method was introduced and the measurement results of strain and stress was given. Meanwhile the finite element analysis was performed for the pressure vessel, which was generally matched with the measurement results. The reliability of strain measurement was verified and the high strength margin of vessel was shown, which would give a good reference value for the follow-up hydraulic tests and strength analysis of reactor pressure vessel. (authors)

Strain sonoelastographic evaluation of biceps muscle intrinsic stiffness after botulinum toxin-A injection.

Science.gov (United States)

Aşkın, Ayhan; Kalaycı, Özlem Tuğçe; Bayram, Korhan Barış; Tosun, Aliye; Demirdal, Ümit Seçil; Atar, Emel; İnci, Mehmet Fatih

2017-01-01

The most commonly used clinical tools for measuring spasticity are modified Ashworth scale (MAS) and Tardieu scale but both yield subjective rather than objective results. Ultrasound elastography (EUS) provides information on tissue stiffness and allows the qualitative or quantitative measurements of the mechanical properties of tissues. To assess the stiffness of biceps brachialis muscles in stroke patients by strain EUS and to investigate the sonoelastographic changes and its correlations with clinical evaluation parameters after botulinum toxin-A (BTA) injections. This is a prospective study. A total of 48 chronic stroke patients requiring BTA injections to biceps brachialis muscles were included in the study. All patients received injections with BTA to biceps brachialis muscles under ultrasound guidance. MAS, goniometric measurements, and strain EUS assessments were performed at preintervention and at 4-week postintervention. Strain index values of biceps muscle on the affected side were significantly increased compared with those on the unaffected side (p < 0.01). At 4 weeks after BTA injection, significant improvements were observed in MAS grades and goniometric measurements (p < 0.05). Statistically significant differences were also found between the MAS grades and strain index values in both pre-/postintervention period (p < 0.01). No significant correlations were observed between clinical parameters and strain EUS findings. Strain EUS is a promising diagnostic tool for assessing stiffness in spastic muscles, in establishing the treatment plan and monitoring the effectiveness of the therapeutic modality.

Evaluation of impedance on biological Tissues using automatic control measurement system

Energy Technology Data Exchange (ETDEWEB)

Kil, Sang Hyeong; Shin, Dong Hoon; Lee, Seong Mo [Pusan National University, Yangsan (Korea, Republic of); Lee, Moo Seok; Kim, Sang Sik [Pusan National University, Busan (Korea, Republic of); Kim, Gun FDo; Lee, Jong Kyu [Pukyung National University, Busan (Korea, Republic of)

2015-08-15

Each biological tissue has endemic electrical characteristics owing to various differences such as those in cellular arrangement or organization form. The endemic electrical characteristics change when any biological change occurs. This work is a preliminary study surveying the changes in the electrical characteristics of biological tissue caused by radiation exposure. For protection against radiation hazards, therefore the electrical characteristics of living tissue were evaluated after development of the automatic control measurement system using LabVIEW. No alteration of biological tissues was observed before and after measurement of the electrical characteristics, and the biological tissues exhibited similar patterns. Through repeated measurements using the impedance/gain-phase analyzer, the coefficient of variation was determined as within 10%. The reproducibility impedance phase difference in electrical characteristics of the biological tissue did not change, and the tissue had resistance. The absolute value of impedance decreased constantly in proportion to the frequency. It has become possible to understand the electrical characteristics of biological tissues through the measurements made possible by the use of the developed.

Evaluation of impedance on biological Tissues using automatic control measurement system

International Nuclear Information System (INIS)

Kil, Sang Hyeong; Shin, Dong Hoon; Lee, Seong Mo; Lee, Moo Seok; Kim, Sang Sik; Kim, Gun FDo; Lee, Jong Kyu

2015-01-01

Each biological tissue has endemic electrical characteristics owing to various differences such as those in cellular arrangement or organization form. The endemic electrical characteristics change when any biological change occurs. This work is a preliminary study surveying the changes in the electrical characteristics of biological tissue caused by radiation exposure. For protection against radiation hazards, therefore the electrical characteristics of living tissue were evaluated after development of the automatic control measurement system using LabVIEW. No alteration of biological tissues was observed before and after measurement of the electrical characteristics, and the biological tissues exhibited similar patterns. Through repeated measurements using the impedance/gain-phase analyzer, the coefficient of variation was determined as within 10%. The reproducibility impedance phase difference in electrical characteristics of the biological tissue did not change, and the tissue had resistance. The absolute value of impedance decreased constantly in proportion to the frequency. It has become possible to understand the electrical characteristics of biological tissues through the measurements made possible by the use of the developed.

Development of a fiber optic pavement subgrade strain measurement system

Science.gov (United States)

Miller, Craig Emerson

2000-11-01

This dissertation describes the development of a fiber optic sensing system to measure strains within the soil subgrade of highway pavements resulting from traffic loads. The motivation to develop such a device include improvements to: (1)all phases of pavement design, (2)theoretical models used to predict pavement performance, and (3)pavement rehabilitation. The design of the sensing system encompasses selecting an appropriate transducer design as well as the development of optimal optical and demodulation systems. The first is spring based, which attempts to match its spring stiffness to that of the soil-data indicate it is not an optimal transducer design. The second transducer implements anchoring plates attached to two telescoping tubes which allows the soil to be compacted to a desired density between the plates to dictate the transducer's behavior. Both transducers include an extrinsic Fabry- Perot cavity to impose the soil strains onto a phase change of the optical signal propagating through the cavity. The optical system includes a low coherence source and allows phase modulation via path length stretching by adding a second interferometer in series with the transducer, resulting in a path matched differential interferometer. A digitally implemented synthetic heterodyne demodulator based on a four step phase stepping algorithm is used to obtain unambiguous soil strain information from the displacement of the Fabry-Perot cavity. The demodulator is calibrated and characterized by illuminating the transducer with a second long coherence source of different wavelength. The transducer using anchoring plates is embedded within cylindrical soil specimens of varying soil types and soil moisture contents. Loads are applied to the specimen and resulting strains are measured using the embedded fiber optic gage and LVDTs attached to the surface of the specimen. This experimental verification is substantiated using a finite element analysis to predict any differences

The measurement of the modal strain fields using digital shearography

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Gomes J.M.

2010-06-01

Full Text Available This work presents a Michelson shearography interferometer configuration associated with stroboscopic double illumination technique for the measurement of modal rotation fields and their strain fields on a clamped circular aluminium plate. The speckle pattern is frozen by the synchronization between the LASER illumination and the modal vibration of the object. The quantitative evaluation is performed for each digital shearogram using a time modulation technique. The setup of double illumination LASER with out-of-plane opposite sensitivity allows the two phase maps measurement of the modal spatial gradient. The modal rotation and strain fields are extracted by the combination of this two digital phase maps. Image processing techniques are applied on the phase maps to obtain full-field measurements using a dedicated post-processing algorithm. Finally, is presented a comparison between the experimental measurement and the numerical solution.

Laser-induced photo-thermal strain imaging

Science.gov (United States)

Choi, Changhoon; Ahn, Joongho; Jeon, Seungwan; Kim, Chulhong

2018-02-01

Vulnerable plaque is the one of the leading causes of cardiovascular disease occurrence. However, conventional intravascular imaging techniques suffer from difficulty in finding vulnerable plaque due to limitation such as lack of physiological information, imaging depth, and depth sensitivity. Therefore, new techniques are needed to help determine the vulnerability of plaque, Thermal strain imaging (TSI) is an imaging technique based on ultrasound (US) wave propagation speed that varies with temperature of medium. During temperature increase, strain occurs in the medium and its variation tendency is depending on the type of tissue, which makes it possible to use for tissue differentiation. Here, we demonstrate laser-induced photo-thermal strain imaging (pTSI) to differentiate tissue using an intravascular ultrasound (IVUS) catheter and a 1210-nm continuous-wave laser for heating lipids intensively. During heating, consecutive US images were obtained from a custom-made phantom made of porcine fat and gelatin. A cross correlation-based speckle-tracking algorithm was then applied to calculate the strain of US images. In the strain images, the positive strain produced in lipids (porcine fat) was clearly differentiated from water-bearing tissue (gelatin). This result shows that laser-induced pTSI could be a new method to distinguish lipids in the plaque and can help to differentiate vulnerability of plaque.

Thermal Conductivity Measurement of Anisotropic Biological Tissue In Vitro

Science.gov (United States)

Yue, Kai; Cheng, Liang; Yang, Lina; Jin, Bitao; Zhang, Xinxin

2017-06-01

The accurate determination of the thermal conductivity of biological tissues has implications on the success of cryosurgical/hyperthermia treatments. In light of the evident anisotropy in some biological tissues, a new modified stepwise transient method was proposed to simultaneously measure the transverse and longitudinal thermal conductivities of anisotropic biological tissues. The physical and mathematical models were established, and the analytical solution was derived. Sensitivity analysis and experimental simulation were performed to determine the feasibility and measurement accuracy of simultaneously measuring the transverse and longitudinal thermal conductivities. The experimental system was set up, and its measurement accuracy was verified by measuring the thermal conductivity of a reference standard material. The thermal conductivities of the pork tenderloin and bovine muscles were measured using the traditional 1D and proposed methods, respectively, at different temperatures. Results indicate that the thermal conductivities of the bovine muscle are lower than those of the pork tenderloin muscle, whereas the bovine muscle was determined to exhibit stronger anisotropy than the pork tenderloin muscle. Moreover, the longitudinal thermal conductivity is larger than the transverse thermal conductivity for the two tissues and all thermal conductivities increase with the increase in temperature. Compared with the traditional 1D method, results obtained by the proposed method are slightly higher although the relative deviation is below 5 %.

Pipeline Bending Strain Measurement and Compensation Technology Based on Wavelet Neural Network

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Rui Li

2016-01-01

Full Text Available The bending strain of long distance oil and gas pipelines may lead to instability of the pipeline and failure of materials, which seriously deteriorates the transportation security of oil and gas. To locate the position of the bending strain for maintenance, an Inertial Measurement Unit (IMU is usually adopted in a Pipeline Inspection Gauge (PIG. The attitude data of the IMU is usually acquired to calculate the bending strain in the pipe. However, because of the vibrations in the pipeline and other system noises, the resulting bending strain calculations may be incorrect. To improve the measurement precision, a method, based on wavelet neural network, was proposed. To test the proposed method experimentally, a PIG with the proposed method is used to detect a straight pipeline. It can be obtained that the proposed method has a better repeatability and convergence than the original method. Furthermore, the new method is more accurate than the original method and the accuracy of bending strain is raised by about 23% compared to original method. This paper provides a novel method for precisely inspecting bending strain of long distance oil and gas pipelines and lays a foundation for improving the precision of inspection of bending strain of long distance oil and gas pipelines.

Measurements and predictions of strain pole figures for uniaxially compressed stainless steel

International Nuclear Information System (INIS)

Larsson, C.; Clausen, B.; Holden, T.M.; Bourke, M.A.M.

2004-01-01

Strain pole figures representative of residual intergranular strains were determined from an -2.98% uniaxially compressed austenitic stainless steel sample. The measurements were made using neutron diffraction on the recently commissioned Spectrometer for Materials Research at Temperature and Stress (SMARTS) at Los Alamos National Laboratory, USA. The measurements were compared with predictions from an elasto-plastic self-consistent model and found to be in good agreement

Measurements and predictions of strain pole figures for uniaxially compressed stainless steel

Energy Technology Data Exchange (ETDEWEB)

Larsson, C. [Division of Engineering Materials, Department of Mechanical Engineering, Linkoeping University, 58183 Linkoeping (Sweden)]. E-mail: clarsson@cfl.rr.com; Clausen, B. [Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Holden, T.M. [Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Bourke, M.A.M. [Los Alamos National Laboratory, Los Alamos, NM 87545 (United States)

2004-09-15

Strain pole figures representative of residual intergranular strains were determined from an -2.98% uniaxially compressed austenitic stainless steel sample. The measurements were made using neutron diffraction on the recently commissioned Spectrometer for Materials Research at Temperature and Stress (SMARTS) at Los Alamos National Laboratory, USA. The measurements were compared with predictions from an elasto-plastic self-consistent model and found to be in good agreement.

Mechanical analysis of the strains generated by water tension in plant stems. Part II: strains in wood and bark and apparent compliance.

Science.gov (United States)

Alméras, Tancrède

2008-10-01

Tree stems shrink in diameter during the day and swell during the night in response to changes in water tension in the xylem. Stem shrinkage can easily be measured in a nondestructive way, to derive continuous information about tree water status. The relationship between the strain and the change in water tension can be evaluated by empirical calibrations, or can be related to the structure of the plant. A mechanical analysis was performed to make this relationship explicit. The stem is modeled as a cylinder made of multiple layers of tissues, including heartwood, sapwood, and inner and outer bark. The effect of changes in water tension on the apparent strain at the surface of a tissue is quantified as a function of parameters defining stem anatomy and the mechanical properties of the tissues. Various possible applications in the context of tree physiology are suggested.

Effect of luminescence transport through adipose tissue on measurement of tissue temperature by using ZnCdS nanothermometers

Science.gov (United States)

Volkova, Elena K.; Yanina, Irina Yu.; Sagaydachnaya, Elena; Konyukhova, Julia G.; Kochubey, Vyacheslav I.; Tuchin, Valery V.

2018-02-01

The spectra of luminescence of ZnCdS nanoparticles (ZnCdS NPs) were measured and analyzed in a wide temperature range: from room to human body and further to a hyperthermic temperature resulting in tissue morphology change. The results show that the signal of luminescence of ZnCdS NPs placed within the tissue is reasonably good sensitive to temperature change and accompanied by phase transitions of lipid structures of adipose tissue. It is shown that the presence of a phase transition in adipose tissue upon its heating (polymorphic transformations of lipids) leads to a nonmonotonic temperature dependence of the intensity of luminescence for the nanoparticles introduced into adipose tissue. This is due to a change in the light scattering by the tissue. The light scattering of adipose tissue greatly distorts the results of temperature measurements. The application of these nanoparticles is possible for temperature measurements in very thin or weakly scattering samples.

Neuromuscular Strain Increases Symptom Intensity in Chronic Fatigue Syndrome.

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Peter C Rowe

Full Text Available Chronic fatigue syndrome (CFS is a complex, multisystem disorder that can be disabling. CFS symptoms can be provoked by increased physical or cognitive activity, and by orthostatic stress. In preliminary work, we noted that CFS symptoms also could be provoked by application of longitudinal neural and soft tissue strain to the limbs and spine of affected individuals. In this study we measured the responses to a straight leg raise neuromuscular strain maneuver in individuals with CFS and healthy controls. We randomly assigned 60 individuals with CFS and 20 healthy controls to either a 15 minute period of passive supine straight leg raise (true neuromuscular strain or a sham straight leg raise. The primary outcome measure was the symptom intensity difference between the scores during and 24 hours after the study maneuver compared to baseline. Fatigue, body pain, lightheadedness, concentration difficulties, and headache scores were measured individually on a 0-10 scale, and summed to create a composite symptom score. Compared to individuals with CFS in the sham strain group, those with CFS in the true strain group reported significantly increased body pain (P = 0.04 and concentration difficulties (P = 0.02 as well as increased composite symptom scores (all P = 0.03 during the maneuver. After 24 hours, the symptom intensity differences were significantly greater for the CFS true strain group for the individual symptom of lightheadedness (P = 0.001 and for the composite symptom score (P = 0.005. During and 24 hours after the exposure to the true strain maneuver, those with CFS had significantly higher individual and composite symptom intensity changes compared to the healthy controls. We conclude that a longitudinal strain applied to the nerves and soft tissues of the lower limb is capable of increasing symptom intensity in individuals with CFS for up to 24 hours. These findings support our preliminary observations that increased mechanical

A three-dimensional strain measurement method in elastic transparent materials using tomographic particle image velocimetry.

Directory of Open Access Journals (Sweden)

Azuma Takahashi

Full Text Available The mechanical interaction between blood vessels and medical devices can induce strains in these vessels. Measuring and understanding these strains is necessary to identify the causes of vascular complications. This study develops a method to measure the three-dimensional (3D distribution of strain using tomographic particle image velocimetry (Tomo-PIV and compares the measurement accuracy with the gauge strain in tensile tests.The test system for measuring 3D strain distribution consists of two cameras, a laser, a universal testing machine, an acrylic chamber with a glycerol water solution for adjusting the refractive index with the silicone, and dumbbell-shaped specimens mixed with fluorescent tracer particles. 3D images of the particles were reconstructed from 2D images using a multiplicative algebraic reconstruction technique (MART and motion tracking enhancement. Distributions of the 3D displacements were calculated using a digital volume correlation. To evaluate the accuracy of the measurement method in terms of particle density and interrogation voxel size, the gauge strain and one of the two cameras for Tomo-PIV were used as a video-extensometer in the tensile test. The results show that the optimal particle density and interrogation voxel size are 0.014 particles per pixel and 40 × 40 × 40 voxels with a 75% overlap. The maximum measurement error was maintained at less than 2.5% in the 4-mm-wide region of the specimen.We successfully developed a method to experimentally measure 3D strain distribution in an elastic silicone material using Tomo-PIV and fluorescent particles. To the best of our knowledge, this is the first report that applies Tomo-PIV to investigate 3D strain measurements in elastic materials with large deformation and validates the measurement accuracy.

Epithelium percentage estimation facilitates epithelial quantitative protein measurement in tissue specimens.

Science.gov (United States)

Chen, Jing; Toghi Eshghi, Shadi; Bova, George Steven; Li, Qing Kay; Li, Xingde; Zhang, Hui

2013-12-01

The rapid advancement of high-throughput tools for quantitative measurement of proteins has demonstrated the potential for the identification of proteins associated with cancer. However, the quantitative results on cancer tissue specimens are usually confounded by tissue heterogeneity, e.g. regions with cancer usually have significantly higher epithelium content yet lower stromal content. It is therefore necessary to develop a tool to facilitate the interpretation of the results of protein measurements in tissue specimens. Epithelial cell adhesion molecule (EpCAM) and cathepsin L (CTSL) are two epithelial proteins whose expressions in normal and tumorous prostate tissues were confirmed by measuring staining intensity with immunohistochemical staining (IHC). The expressions of these proteins were measured by ELISA in protein extracts from OCT embedded frozen prostate tissues. To eliminate the influence of tissue heterogeneity on epithelial protein quantification measured by ELISA, a color-based segmentation method was developed in-house for estimation of epithelium content using H&E histology slides from the same prostate tissues and the estimated epithelium percentage was used to normalize the ELISA results. The epithelium contents of the same slides were also estimated by a pathologist and used to normalize the ELISA results. The computer based results were compared with the pathologist's reading. We found that both EpCAM and CTSL levels, measured by ELISA assays itself, were greatly affected by epithelium content in the tissue specimens. Without adjusting for epithelium percentage, both EpCAM and CTSL levels appeared significantly higher in tumor tissues than normal tissues with a p value less than 0.001. However, after normalization by the epithelium percentage, ELISA measurements of both EpCAM and CTSL were in agreement with IHC staining results, showing a significant increase only in EpCAM with no difference in CTSL expression in cancer tissues. These results

Effect-independent measures of tissue response to fractionated radiation

International Nuclear Information System (INIS)

Thames, H.D.

1984-01-01

Tissue repair factors are measures of sparing from dose fractionation, in the absence of proliferation. A desirable feature of any repair factor is that it be independent of the level of injury induced in the tissue, since otherwise the comparison of tissues on the basis of the factor would not be meaningful. The repair factors F/sub R/ and F/sub rec/ are increasing functions of D/sub 1/, and depend on level of skin reaction after fractionated radiation. By contrast, β/α is effect-independent as a measure of repair capacity in skin, gut, and bone marrow. For late fibrotic reactions in the kidney, there was an increase in β/α with increased levels of injury that was statistically insignificant. The halftime, T/sub 1/2/, for intracellular repair processes in tissues is a measure of repair kinetics. Effect-independence is defend for T/sub 1/2/ as independence from size of dose per fraction. T/sub 1/2/ is independent of fraction size in skin, gut, and spinal cord, and is longer (1.5 hours) in the late-reacting tissues (lung and spinal cord) than in those that react acutely (less than 1 hour), with skin as the exception (1.3 hours). Therefore, early and late-responding normal tissues may be distinguished in terms of both repair capacity and repair kinetics: repair is slower in late-responding tissues, which are also more sensitive to changes in dose fractionation

Measurements of mechanical anisotropy in brain tissue and implications for transversely isotropic material models of white matter

Science.gov (United States)

Feng, Yuan; Okamoto, Ruth J.; Namani, Ravi; Genin, Guy M.; Bayly, Philip V.

2013-01-01

White matter in the brain is structurally anisotropic, consisting largely of bundles of aligned, myelin-sheathed axonal fibers. White matter is believed to be mechanically anisotropic as well. Specifically, transverse isotropy is expected locally, with the plane of isotropy normal to the local mean fiber direction. Suitable material models involve strain energy density functions that depend on the I4 and I5 pseudo-invariants of the Cauchy–Green strain tensor to account for the effects of relatively stiff fibers. The pseudo-invariant I4 is the square of the stretch ratio in the fiber direction; I5 contains contributions of shear strain in planes parallel to the fiber axis. Most, if not all, published models of white matter depend on I4 but not on I5. Here, we explore the small strain limits of these models in the context of experimental measurements that probe these dependencies. Models in which strain energy depends on I4 but not I5 can capture differences in Young’s (tensile) moduli, but will not exhibit differences in shear moduli for loading parallel and normal to the mean direction of axons. We show experimentally, using a combination of shear and asymmetric indentation tests, that white matter does exhibit such differences in both tensile and shear moduli. Indentation tests were interpreted through inverse fitting of finite element models in the limit of small strains. Results highlight that: (1) hyperelastic models of transversely isotropic tissues such as white matter should include contributions of both the I4 and I5 strain pseudo-invariants; and (2) behavior in the small strain regime can usefully guide the choice and initial parameterization of more general material models of white matter. PMID:23680651

Elastic properties and strain-to-crack-initiation of calcium phosphate bone cements: Revelations of a high-resolution measurement technique.

Science.gov (United States)

Ajaxon, Ingrid; Acciaioli, Alice; Lionello, Giacomo; Ginebra, Maria-Pau; Öhman-Mägi, Caroline; Baleani, Massimiliano; Persson, Cecilia

2017-10-01

Calcium phosphate cements (CPCs) should ideally have mechanical properties similar to those of the bone tissue the material is used to replace or repair. Usually, the compressive strength of the CPCs is reported and, more rarely, the elastic modulus. Conversely, scarce or no data are available on Poisson's ratio and strain-to-crack-initiation. This is unfortunate, as data on the elastic response is key to, e.g., numerical model accuracy. In this study, the compressive behaviour of brushite, monetite and apatite cements was fully characterised. Measurement of the surface strains was done using a digital image correlation (DIC) technique, and compared to results obtained with the commonly used built-in displacement measurement of the materials testers. The collected data showed that the use of fixed compression platens, as opposed to spherically seated ones, may in some cases underestimate the compressive strength by up to 40%. Also, the built-in measurements may underestimate the elastic modulus by up to 62% as compared to DIC measurements. Using DIC, the brushite cement was found to be much stiffer (24.3 ± 2.3GPa) than the apatite (13.5 ± 1.6GPa) and monetite (7.1 ± 1.0GPa) cements, and elastic moduli were inversely related to the porosity of the materials. Poisson's ratio was determined to be 0.26 ± 0.02 for brushite, 0.21 ± 0.02 for apatite and 0.20 ± 0.03 for monetite. All investigated CPCs showed low strain-to-crack-initiation (0.17-0.19%). In summary, the elastic modulus of CPCs is substantially higher than previously reported and it is concluded that an accurate procedure is a prerequisite in order to properly compare the mechanical properties of different CPC formulations. It is recommended to use spherically seated platens and measuring the strain at a relevant resolution and on the specimen surface. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Application of fiber optic grating strain sensor for measurement of strain under irradiation environment

International Nuclear Information System (INIS)

Kaji, Y.; Matsui, Y.; Kita, S.; Ide, H.; Tsukada, T.; Tsuji, H.

2001-01-01

In Japan Atomic Energy Research Institute (JAERI), in-pile strain measurement techniques have been developed using Japan Materials Testing Reactor (JMTR). In order to evaluate the performance of fiber optic grating sensor under irradiation environment, heat-up and performance tests at elevated temperature before irradiation and in-pile tests were performed in JMTR. (author)

NDE (Nondestructive examination) by ultrasonic, photo-elastic, strain measuring and FEM (Finite Element Method)

International Nuclear Information System (INIS)

Gu Fangyu; Zeng Xiao

1990-01-01

It is considered impossible to inspect flaw by using ordinary mechanical measuring methods. In this paper, it is found that the stree and strain distortions of pressure vessel with 2D linear shape crack in the deep location appear the 'cat effect' on the surface of stracture, and that the location and size of the crack can be determined with strain measuring and FEM according to 'cat effect' of strain distortion

Real-time contrast-enhanced ultrasound determination of microvascular blood volume in abdominal subcutaneous adipose tissue in man. Evidence for adipose tissue capillary recruitment

DEFF Research Database (Denmark)

Tobin, L; Simonsen, L; Bülow, J

2010-01-01

The adipose tissue metabolism is dependent on its blood perfusion. During lipid mobilization e.g. during exercise and during lipid deposition e.g. postprandial, adipose tissue blood flow is increased. This increase in blood flow may involve capillary recruitment in the tissue. We investigated...... of ultrasound contrast agent to establish the reproducibility of the technique. In nine subjects, the effect of an oral glucose load on blood flow and microvascular volume was measured in abdominal subcutaneous adipose tissue and forearm skeletal muscle. ¹³³Xe washout and venous occlusion strain......-gauge plethysmography was used to measure the adipose tissue and forearm blood flow, respectively. Ultrasound signal intensity of the first plateau phases was 27 ± dB in the abdominal subcutaneous adipose tissue and 18 ± 2 dB (P

Tissue oxygenation and haemodynamics measurement with spatially resolved NIRS

Science.gov (United States)

Zhang, Y.; Scopesi, F.; Serra, G.; Sun, J. W.; Rolfe, P.

2010-08-01

We describe the use of Near Infrared Spectroscopy (NIRS) for the non-invasive investigation of changes in haemodynamics and oxygenation of human peripheral tissues. The goal was to measure spatial variations of tissue NIRS oxygenation variables, namely deoxy-haemoglobin (HHb), oxy-haemoglobin (HbO2), total haemoglobin (HbT), and thereby to evaluate the responses of the peripheral circulation to imposed physiological challenges. We present a skinfat- muscle heterogeneous tissue model with varying fat thickness up to 15mm and a Monte Carlo simulation of photon transport within this model. The mean partial path length and the mean photon visit depth in the muscle layer were derived for different source-detector spacing. We constructed NIRS instrumentation comprising of light-emitting diodes (LED) as light sources at four wavelengths, 735nm, 760nm, 810nm and 850nm and sensitive photodiodes (PD) as the detectors. Source-detector spacing was varied to perform measurements at different depths within forearm tissue. Changes in chromophore concentration in response to venous and arterial occlusion were calculated using the modified Lambert-Beer Law. Studies in fat and thin volunteers indicated greater sensitivity in the thinner subjects for the tissue oxygenation measurement in the muscle layer. These results were consistent with those found using Monte Carlo simulation. Overall, the results of this investigation demonstrate the usefulness of the NIRS instrument for deriving spatial information from biological tissues.

Emergent material properties of developing epithelial tissues.

Science.gov (United States)

Machado, Pedro F; Duque, Julia; Étienne, Jocelyn; Martinez-Arias, Alfonso; Blanchard, Guy B; Gorfinkiel, Nicole

2015-11-23

Force generation and the material properties of cells and tissues are central to morphogenesis but remain difficult to measure in vivo. Insight is often limited to the ratios of mechanical properties obtained through disruptive manipulation, and the appropriate models relating stress and strain are unknown. The Drosophila amnioserosa epithelium progressively contracts over 3 hours of dorsal closure, during which cell apices exhibit area fluctuations driven by medial myosin pulses with periods of 1.5-6 min. Linking these two timescales and understanding how pulsatile contractions drive morphogenetic movements is an urgent challenge. We present a novel framework to measure in a continuous manner the mechanical properties of epithelial cells in the natural context of a tissue undergoing morphogenesis. We show that the relationship between apicomedial myosin fluorescence intensity and strain during fluctuations is consistent with a linear behaviour, although with a lag. We thus used myosin fluorescence intensity as a proxy for active force generation and treated cells as natural experiments of mechanical response under cyclic loading, revealing unambiguous mechanical properties from the hysteresis loop relating stress to strain. Amnioserosa cells can be described as a contractile viscoelastic fluid. We show that their emergent mechanical behaviour can be described by a linear viscoelastic rheology at timescales relevant for tissue morphogenesis. For the first time, we establish relative changes in separate effective mechanical properties in vivo. Over the course of dorsal closure, the tissue solidifies and effective stiffness doubles as net contraction of the tissue commences. Combining our findings with those from previous laser ablation experiments, we show that both apicomedial and junctional stress also increase over time, with the relative increase in apicomedial stress approximately twice that of other obtained measures. Our results show that in an epithelial

An Approach for the Dynamic Measurement of Ring Gear Strains of Planetary Gearboxes Using Fiber Bragg Gratings

Directory of Open Access Journals (Sweden)

Hang Niu

2017-12-01

Full Text Available The strain of the ring gear can reflect the dynamic characteristics of planetary gearboxes directly, which makes it an ideal signal to monitor the health condition of the gearbox. To overcome the disadvantages of traditional methods, a new approach for the dynamic measurement of ring gear strains using fiber Bragg gratings (FBGs is proposed in this paper. Firstly, the installation of FBGs is determined according to the analysis for the strain distribution of the ring gear. Secondly, the parameters of the FBG are determined in consideration of the accuracy and sensitivity of the measurement as well as the size of the ring gear. The strain measured by the FBG is then simulated under non-uniform strain field conditions. Thirdly, a dynamic measurement system is built and tested. Finally, the strains of the ring gear are measured in a planetary gearbox under normal and faulty conditions. The experimental results showed good agreement with the theoretical results in values, trends, and the fault features can be seen from the time domain of the measured strain signal, which proves that the proposed method is feasible for the measurement of the ring gear strains of planetary gearboxes.

An Approach for the Dynamic Measurement of Ring Gear Strains of Planetary Gearboxes Using Fiber Bragg Gratings.

Science.gov (United States)

Niu, Hang; Zhang, Xiaodong; Hou, Chenggang

2017-12-16

The strain of the ring gear can reflect the dynamic characteristics of planetary gearboxes directly, which makes it an ideal signal to monitor the health condition of the gearbox. To overcome the disadvantages of traditional methods, a new approach for the dynamic measurement of ring gear strains using fiber Bragg gratings (FBGs) is proposed in this paper. Firstly, the installation of FBGs is determined according to the analysis for the strain distribution of the ring gear. Secondly, the parameters of the FBG are determined in consideration of the accuracy and sensitivity of the measurement as well as the size of the ring gear. The strain measured by the FBG is then simulated under non-uniform strain field conditions. Thirdly, a dynamic measurement system is built and tested. Finally, the strains of the ring gear are measured in a planetary gearbox under normal and faulty conditions. The experimental results showed good agreement with the theoretical results in values, trends, and the fault features can be seen from the time domain of the measured strain signal, which proves that the proposed method is feasible for the measurement of the ring gear strains of planetary gearboxes.

Direct Strain and Slope and Slope Measurement Using 2D DSPSI

International Nuclear Information System (INIS)

Dandach, W.; Molimard, J.; Picart, P.

2011-01-01

Large variety of optical full-field measurement techniques are being developed and applied to solve mechanical problems. Since each technique possesses its own merits, it is important to know the capabilities and limitations of such techniques. Among these optical full-field methods, interferometry techniques take an important place. They are based on illumination with coherent light (laser). In shearing interferometry the difference of the out of-plane displacement in two neighboring object points is directly measured. Since object displacement does not result in interferometry fringes, the method is suited for localization of strain concentrations and is indeed used in industry for this purpose. DSPSI possesses the advantage over conventional out-of-plane displacement-sensitive interferometry, that only a single difference of the unwrapped phase map is required to obtain flexural strains, thereby relieving problems with noise and reduction in the field of view. A first work in this domain (DSPSI) [1] was made in 1973, later recent studies emerged to provide a quantitative system of measurements [2]. This work aims to present the results of strain and slope measurements using digital speckle pattern shearing interferometry (DSPSI). (author)

Validation Tests of Fiber Optic Strain-Based Operational Shape and Load Measurements

Science.gov (United States)

Bakalyar, John A.; Jutte, Christine

2012-01-01

Aircraft design has been progressing toward reduced structural weight to improve fuel efficiency, increase performance, and reduce cost. Lightweight aircraft structures are more flexible than conventional designs and require new design considerations. Intelligent sensing allows for enhanced control and monitoring of aircraft, which enables increased structurally efficiency. The NASA Dryden Flight Research Center (DFRC) has developed an instrumentation system and analysis techniques that combine to make distributed structural measurements practical for lightweight vehicles. Dryden's Fiber Optic Strain Sensing (FOSS) technology enables a multitude of lightweight, distributed surface strain measurements. The analysis techniques, referred to as the Displacement Transfer Functions (DTF) and Load Transfer Functions (LTF), use surface strain values to calculate structural deflections and operational loads. The combined system is useful for real-time monitoring of aeroelastic structures, along with many other applications. This paper describes how the capabilities of the measurement system were demonstrated using subscale test articles that represent simple aircraft structures. Empirical FOSS strain data were used within the DTF to calculate the displacement of the article and within the LTF to calculate bending moments due to loads acting on the article. The results of the tests, accuracy of the measurements, and a sensitivity analysis are presented.

Development of a strain measurement method for non-plane specimens by means of computer picture processing

International Nuclear Information System (INIS)

Yoshioka, Akira; Soneda, Naoki; Yagawa, Genki; Miyoshi, Akio.

1988-01-01

Integrity Tests of the Fast Breeder Reactor components are often conducted at an elevated temperature, say 550deg C. Since high-temperature strain measurement using special strain gauges is costly and unappropriate for large and repeated strains, the authors have developed an optical strain measurement method and system based on computer picture processing and the triangulation principle. The present method enables us to measure the strain in specimen with curved surfaces. Its operation is also easy, because of the automatic distinction of marks from noises. The verification tests with a plate specimen and a cylindrical one are performed under elevated temperatures. The results show that the present method is very suitable to the tests under elevated temperatures and that the measurement error of strain is within 0.2 % (2000μ), which is reasonable considering the limitation of hardware. (author)

The impact of hydration changes in fresh bio-tissue on THz spectroscopic measurements

International Nuclear Information System (INIS)

Png, G M; Ng, B W-H; Mickan, S P; Abbott, D; Choi, J W; Zhang, X-C

2008-01-01

We present a study of how residual hydration in fresh rat tissue samples can vastly alter their extracted terahertz (THz) optical properties and influence their health assessment. Fresh (as opposed to preserved) tissue most closely mimics in vivo conditions, but high water content creates many challenges for tissue handling and THz measurement. Our THz measurements of fresh tissue over time highlight the effect of tissue hydration on tissue texture and dimension, the latter directly influencing the accuracy of calculated optical properties. We then introduce lyophilization (freeze drying) as a viable solution for overcoming hydration and freshness problems. Lyophilization removes large amounts of water while retaining sample freshness. In addition, lyophilized tissue samples are easy to handle and their textures and dimensions do not vary over time, allowing for consistent and stable THz measurements. A comparison of lyophilized and fresh tissue shows for the first time that freeze drying may be one way of overcoming tissue hydration issues while preserving tissue cellular structure. Finally, we compare THz measurements from fresh tissue against necrotic tissue to verify freshness over time. Indeed, THz measurements from fresh and necrotic tissues show marked differences

Measuring techniques for autogenous strain of cement paste

DEFF Research Database (Denmark)

Lura, Pietro; Jensen, Ole Mejlhede

2007-01-01

Volumetric measurement of autogenous strain is frequently performed by placing the fresh cement paste in a rubber membrane submerged in water. The volume change of the cement paste is measured by the amount of water displaced by the submerged sample. Volumetric and linear measurements of autogenous...... of the volumetric method. Water absorption is driven by a lowering of the water activity in the cement paste due to dissolved salts in the pore fluid and to self-desiccation. From the moment of casting, significant water uptake was registered in all experiments. This water uptake influenced the volumetric...... on the same cement pastes....

From measurements errors to a new strain gauge design for composite materials

DEFF Research Database (Denmark)

Mikkelsen, Lars Pilgaard; Salviato, Marco; Gili, Jacopo

2015-01-01

Significant over-prediction of the material stiffness in the order of 1-10% for polymer based composites has been experimentally observed and numerical determined when using strain gauges for strain measurements instead of non-contact methods such as digital image correlation or less stiff method...

Resonance sensor measurements of stiffness variations in prostate tissue in vitro--a weighted tissue proportion model.

Science.gov (United States)

Jalkanen, Ville; Andersson, Britt M; Bergh, Anders; Ljungberg, Börje; Lindahl, Olof A

2006-12-01

Prostate cancer is the most common type of cancer in men in Europe and the US. The methods to detect prostate cancer are still precarious and new techniques are needed. A piezoelectric transducer element in a feedback system is set to vibrate with its resonance frequency. When the sensor element contacts an object a change in the resonance frequency is observed, and this feature has been utilized in sensor systems to describe physical properties of different objects. For medical applications it has been used to measure stiffness variations due to various patho-physiological conditions. In this study the sensor's ability to measure the stiffness of prostate tissue, from two excised prostatectomy specimens in vitro, was analysed. The specimens were also subjected to morphometric measurements, and the sensor parameter was compared with the morphology of the tissue with linear regression. In the probe impression interval 0.5-1.7 mm, the maximum R(2) > or = 0.60 (p sensor was pressed, the greater, i.e., deeper, volume it sensed. Tissue sections deeper in the tissue were assigned a lower mathematical weighting than sections closer to the sensor probe. It is concluded that cancer increases the measured stiffness as compared with healthy glandular tissue, but areas with predominantly stroma or many stones could be more difficult to differ from cancer.

Stress and strain measurements in solids

International Nuclear Information System (INIS)

Askegaard, V.

1978-01-01

A design basis is given for stress- and strain cells to be used in a solid either externally loaded or with a stressfree strain field (for example shrinkage). A stress- and a strain cell has been designed for use in granular materials. Calibration tests show either good or reasonably good correspondance with calculated values. (orig.) [de

Temperature and strain measurements in concrete using micro-structure optical fiber sensors

Energy Technology Data Exchange (ETDEWEB)

Areias, Lou [EURIDICE/SCK - CEN, Mol (Belgium); Vrije Univ. Brussels (Belgium); Geernaert, Thomas; Sulejmani, Sanne [Vrije Univ. Brussels (Belgium); and others

2015-07-01

A recent test carried out to evaluate the construction feasibility of the Belgian supercontainer concept incorporated several types of state-of-the-art sensors and innovative monitoring techniques, including the use of different types of optical fiber sensors. One of these is a relatively new type of sensor developed by the Brussels Photonics Team (B-PHOT) of the Vrije Universiteit Brussel. The sensor uses highly birefringent microstructured optical fibers equipped with fiber Bragg gratings (MOFBGs) sensors. They were embedded in a carbon-fiber reinforced composite plate to provide protection against the concrete's highly alkaline environment, facilitate installation in the concrete mould and allow the transfer of strain onto the fiber. The double reflection spectrum of the MOFBGs allows monitoring strain and temperature simultaneously. This paper presents results of temperature and strain measurements obtained with MOFBG sensors during a {sup 1}/{sub 2}-scale test performed in 2013. The results compare well with similar measurements obtained using conventional thermocouples and vibrating wire strain gauges.

Fast recovery strain measurements in a nuclear test environment

International Nuclear Information System (INIS)

Kitchen, W.R.; Nauman, W.J.; Vollmer, D.W.

1979-01-01

The recovery of early-time (50 μs or less) strain gage data on structural response experiments in underground nuclear tests has been a continuing problem for experimenters at the Nevada Test Site. Strain measurement is one of the primary techniques used to obtain experimental data for model verification and correlation with predicted effects. Peak strains generally occur within 50 to 100 μs of the radiation exposure. Associated with the exposure is an intense electromagnetic impulse that produces potentials of kilovolts and currents of kiloamperes on the experimental structures. For successful operation, the transducer and associated recording system must recover from the initial noise overload and accurately track the strain response within about 50 μs of the nuclear detonation. A gaging and fielding technique and a recording system design that together accomplish these objectives are described. Areas discussed include: (1) noise source model; (2) experimental cassette design, gage application, grounding, and shielding; (3) cable design and shielding between gage and recorder; (4) recorder design including signal conditioner/amplifier, digital encoder, buffer memory, and uphole data transmission; and (5) samples of experimental data

Measurement of tissue-radiation dosage using a thermal steady-state elastic shear wave.

Science.gov (United States)

Chang, Sheng-Yi; Hsieh, Tung-Sheng; Chen, Wei-Ru; Chen, Jin-Chung; Chou, Chien

2017-08-01

A biodosimeter based on thermal-induced elastic shear wave (TIESW) in silicone acellular porcine dermis (SAPD) at thermal steady state has been proposed and demonstrated. A square slab SAPD treated with ionizing radiation was tested. The SAPD becomes a continuous homogeneous and isotropic viscoelastic medium due to the generation of randomly coiled collagen fibers formed from their bundle-like structure in the dermis. A harmonic TIESW then propagates on the surface of the SAPD as measured by a nanometer-scaled strain-stress response under thermal equilibrium conditions at room temperature. TIESW oscillation frequency was noninvasively measured in real time by monitoring the transverse displacement of the TIESW on the SAPD surface. Because the elastic shear modulus is highly sensitive to absorbed doses of ionizing radiation, this proposed biodosimeter can become a highly sensitive and noninvasive method for quantitatively determining tissue-absorbed dosage in terms of TIESW’s oscillation frequency. Detection sensitivity at 1 cGy and dynamic ranges covering 1 to 40 cGy and 80 to 500 cGy were demonstrated.

Strain measurements during pressurized thermal shock experiment

International Nuclear Information System (INIS)

Tarso Vida Gomes, P. de; Julio Ricardo Barreto Cruz; Tanius Rodrigues Mansur; Denis Henrique Bianchi Scaldaferri; Miguel Mattar Neto

2005-01-01

For the life extension of nuclear power plants, the residual life of most of their components must be evaluated along all their operating time. Concerning the reactor pressure vessel, the pressurized thermal shock (PTS) is a very important event to be considered. For better understanding the effects of this kind of event, tests are made. The approach described here consisted of building a simplified in-scale physical model of the reactor pressure vessel, submitting it to the actual operating temperature and pressure conditions and provoking a thermal shock by means of cold water flow in its external surface. To conduct such test, the Nuclear Technology Development Center (CDTN) has been conducting several studies related to PTS and has also built a laboratory that has made possible the simulation of the PTS loading conditions. Several cracks were produced in the external surface of the reactor pressure vessel model. Strain gages were fixed by means of electrical discharge welding over the cracks regions in both external and internal surfaces. The temperature was monitored in 10 points across the vessel wall. The internal pressure was manually controlled and monitored using a pressure transducer. Two PTS experiments were conducted and this paper presents the strain measurement procedures applied to the reactor pressure vessel model, during the PTS, using strain gages experimental methodology. (authors)

Evaluation of resilient abutment components on measured strain using dynamic loading conditions.

Science.gov (United States)

Morton, D; Stanford, C M; Aquilino, S A

1998-07-01

Factors that affect transmission of strain from prostheses to bone may affect the long-term success of loaded implants. Current in vitro models are theoretically predictive (finite element modeling) or facsimile (photoelastic) in nature. A more clinically relevant in vitro model for strain evaluation should be investigated. This study attempted: (1) to validate a human cadaver bone model for vitro measurement of cortical bone strain, and (2) to evaluate the effect on cortical strain measurements of a resilient plastic component incorporated within a titanium implant in response to variable dynamic loading. Two IMZ (Interpore International) abutment alternatives were used: the titanium Abutment Complete and the polyoxymethylene Intra-mobile Element. The model system consisted of two implants placed in unfixed human cadaver ulna bone to simulate an implant bound edentulous region. Four biaxial rosette strain gauges simultaneously recorded cortical bone strain immediately mesial and distal to each implant. During experimentation a simulated prosthetic framework supported by either titanium or polyoxymethylene abutments was dynamically loaded 6 min from the terminal abutment along a cantilever extension. Cyclic nominal peak loads were applied with a materials testing machine at 20-N intervals from 20 to 200 N at a crosshead speed of 5 mm/minute. The protocol allowed frequency of load application to vary. A Newtonian linear correlation (r2 > or = 0.98) between load application and strain output was determined for each gauge position except for the terminal gauge located opposite the cantilever. Cortical strains recorded were within reported physiologic ranges involved in bone modeling and remodeling. Further, the polyoxymethylene abutment components did not result in reduction of peak microstrain at any gauge position. The Intra-mobile Element abutments, however, did increase the time required to complete 10 loading cycles when compared with the titanium Abutment

Internal strain measurement using pulsed neutron diffraction at LANSCE

International Nuclear Information System (INIS)

Goldstone, J.A.; Bourke, M.A.M.; Shi, N.

1994-01-01

The presence of residual stress in engineering components can effect their mechanical properties and structural integrity. Neutron diffraction in the only technique that can make nondestructive measurements in the interior of components. By recording the change in crystalline lattice spacings, elastic strains can be measured for individual lattice reflections. Using a pulsed neutron source, all lattice reflections are recorded in each measurement, which allows for easy examination of heterogeneous materials such as metal matrix composites. Measurements made at the Manuel Lujan Jr. Neutron Scattering Center (LANSCE) demonstrate the potential at pulsed sources for in-situ stress measurements at ambient and elevated temperatures

Up-taper-based Mach-Zehnder interferometer for temperature and strain simultaneous measurement.

Science.gov (United States)

Kang, Zexin; Wen, Xiaodong; Li, Chao; Sun, Jiang; Wang, Jing; Jian, Shuisheng

2014-04-20

A novel all-fiber sensing configuration for simultaneous measurements of temperature and strain based on the up-taper Mach-Zehnder interferometer (MZI) with an in-line embedded fiber Bragg grating (FBG) is proposed and experimentally demonstrated. This configuration consists of two up-tapers fabricated by an excessive fusion splicing method and a short segment of inscribed FBG. Due to the different responses of the up-taper MZI and the FBG to the uniform variation of temperature and strain, the simultaneous measurement for these two variables could be achieved by real-time monitoring the transmission spectrum. For 0.01 nm wavelength resolution, a resolution of 0.311°C in temperature can be achieved, and the average strain resolution is 10.07 με.

Measurement of stress strain and vibrational properties of tendons

Science.gov (United States)

Revel, Gian Marco; Scalise, Alessandro; Scalise, Lorenzo

2003-08-01

The authors present a new non-intrusive experimental procedure based on laser techniques for the measurement of mechanical properties of tendons. The procedure is based on the measurement of the first resonance frequency of the tendon by laser Doppler vibrometry during in vitro tensile experiments, with the final aim of establishing a measurement procedure to perform the mechanical characterization of tendons by extracting parameters such as the resonance frequency, also achievable during in vivo investigation. The experimental procedure is reported, taking into account the need to simulate the physiological conditions of the Achilles tendon, and the measurement technique used for the non-invasive determination of tendon cross-sectional area during tensile vibration tests at different load levels is described. The test procedure is based on a tensile machine, which measures longitudinal tendons undergoing controlled load conditions. Cross-sectional area is measured using a new non-contact procedure for the measurement of tendon perimeter (repeatability of 99% and accuracy of 2%). For each loading condition, vibration resonance frequency and damping, cross-sectional area and tensile force are measured, allowing thus a mechanical characterization of the tendon. Tendon stress-strain curves are reported. Stress-strain curves have been correlated to the first vibration resonance frequency and damping of the tendon measured using a single-point laser Doppler vibrometer. Moreover, experimental results have been compared with a theoretical model of a vibrating cord showing discrepancies. In vitro tests are reported, demonstrating the validity of the method for the comparison of different aged rabbit tendons.

Design and Testing of the Strain Transducer for Measuring Deformations of Pipelines Operating in the Mining-deformable Ground Environment

Directory of Open Access Journals (Sweden)

Gawedzki Waclaw

2015-10-01

Full Text Available Design and laboratory test results of the strain transducer intended for monitoring and assessing stress states of pipelines sited in mining areas are presented in this paper. This transducer allows measuring strains of pipelines subjected to external forces - being the mining operations effect. Pipeline strains can have a direct influence on a tightness loss and penetration of the transported fluid into the environment. The original strain gauge transducer was proposed for performing measurements of strains. It allows measuring circumferential strains and determining the value and direction of the main longitudinal strain. This strain is determined on the basis of measuring component longitudinal strains originating from axial forces and the resultant bending moment. The main purpose of investigations was the experimental verification of the possibility of applying the strain transducer for measuring strains of polyethylene pipelines. The obtained results of the transducer subjected to influences of tensile and compression forces are presented and tests of relaxation properties of polyethylene are performed.

Three-Axis Distributed Fiber Optic Strain Measurement in 3D Woven Composite Structures

Science.gov (United States)

Castellucci, Matt; Klute, Sandra; Lally, Evan M.; Froggatt, Mark E.; Lowry, David

2013-01-01

Recent advancements in composite materials technologies have broken further from traditional designs and require advanced instrumentation and analysis capabilities. Success or failure is highly dependent on design analysis and manufacturing processes. By monitoring smart structures throughout manufacturing and service life, residual and operational stresses can be assessed and structural integrity maintained. Composite smart structures can be manufactured by integrating fiber optic sensors into existing composite materials processes such as ply layup, filament winding and three-dimensional weaving. In this work optical fiber was integrated into 3D woven composite parts at a commercial woven products manufacturing facility. The fiber was then used to monitor the structures during a VARTM manufacturing process, and subsequent static and dynamic testing. Low cost telecommunications-grade optical fiber acts as the sensor using a high resolution commercial Optical Frequency Domain Reflectometer (OFDR) system providing distributed strain measurement at spatial resolutions as low as 2mm. Strain measurements using the optical fiber sensors are correlated to resistive strain gage measurements during static structural loading. Keywords: fiber optic, distributed strain sensing, Rayleigh scatter, optical frequency domain reflectometry

In-situ measurement of texture and elastic strains with HIPPO-CRATES

International Nuclear Information System (INIS)

Hartig, Ch.; Vogel, S.C.; Mecking, H.

2006-01-01

In this paper, the micromechanical interaction between constituents of a metallic material during elastic and plastic deformation are analyzed by comparing experimental results with modeling predictions. This comparison aims at determining the locally acting internal stresses, the spatial distribution of strains and the rules allowing deriving the macroscopic behavior of the material from the behavior of its microscopic constituents. We report the application of a new deformation apparatus CRATES, which allows measuring texture and crystal lattice spacings, and from these crystal lattice strains, using neutron diffraction. From the in-situ measured elastic lattice strains ε hkl the corresponding local stresses can be derived. The deformation apparatus allows uni-axial tensile or compressive deformation up to 100 kN and is specifically designed for use in the HIPPO neutron time-of-flight diffractometer. In this paper, we report initial results on an iron-copper model system (Fe100, Fe33Cu67, Fe67Cu33, vol.%) and commercial magnesium alloys (Mg-AZ31 and Mg-AZ80). Finite element calculations using a crystal-plastic constitutive law, allowing for shear and hardening of crystallographic slip-systems, were used for the interpretation of the measurements

Optical sensor for heat conduction measurement in biological tissue

International Nuclear Information System (INIS)

Gutierrez-Arroyo, A; Sanchez-Perez, C; Aleman-Garcia, N

2013-01-01

This paper presents the design of a heat flux sensor using an optical fiber system to measure heat conduction in biological tissues. This optoelectronic device is based on the photothermal beam deflection of a laser beam travelling in an acrylic slab this deflection is measured with a fiber optic angle sensor. We measure heat conduction in biological samples with high repeatability and sensitivity enough to detect differences in tissues from three chicken organs. This technique could provide important information of vital organ function as well as the detect modifications due to degenerative diseases or physical damage caused by medications or therapies.

Strain Measurements of Chondrules and Refraction Inclusion in Allende

Science.gov (United States)

Tait, Alastair W.; Fisher, Kent R.; Simon, Justin I.

2013-01-01

This study uses traditional strain measurement techniques, combined with X-ray computerized tomography (CT), to evaluate petrographic evidence in the Allende CV3 chondrite for preferred orientation and to measure strain in three dimensions. The existence of petrofabrics and lineations was first observed in carbonaceous meteorites in the 1960's. Yet, fifty years later only a few studies have reported that meteorites record such features. Impacts are often cited as the mechanism for this feature, although plastic deformation from overburden and nebular imbrication have also been proposed. Previous work conducted on the Leoville CV3 and the Parnallee LL3 chondrites, exhibited a minimum uniaxial shortening of 33% and 21%, respectively. Petrofabrics in Allende CV3 have been looked at before; previous workers using Electron Back Scatter Diffraction (EBSD) found a major-axis alignment of olivine inside dark inclusions and an "augen"-like preferred orientation of olivine grains around more competent chondrules

Breast tissue classification using x-ray scattering measurements and multivariate data analysis

Science.gov (United States)

Ryan, Elaine A.; Farquharson, Michael J.

2007-11-01

This study utilized two radiation scatter interactions in order to differentiate malignant from non-malignant breast tissue. These two interactions were Compton scatter, used to measure the electron density of the tissues, and coherent scatter to obtain a measure of structure. Measurements of these parameters were made using a laboratory experimental set-up comprising an x-ray tube and HPGe detector. The breast tissue samples investigated comprise five different tissue classifications: adipose, malignancy, fibroadenoma, normal fibrous tissue and tissue that had undergone fibrocystic change. The coherent scatter spectra were analysed using a peak fitting routine, and a technique involving multivariate analysis was used to combine the peak fitted scatter profile spectra and the electron density values into a tissue classification model. The number of variables used in the model was refined by finding the sensitivity and specificity of each model and concentrating on differentiating between two tissues at a time. The best model that was formulated had a sensitivity of 54% and a specificity of 100%.

Breast tissue classification using x-ray scattering measurements and multivariate data analysis

Energy Technology Data Exchange (ETDEWEB)

Ryan, Elaine A; Farquharson, Michael J [School of Allied Health Sciences, City University, Charterhouse Square, London EC1M 6PA (United Kingdom)

2007-11-21

This study utilized two radiation scatter interactions in order to differentiate malignant from non-malignant breast tissue. These two interactions were Compton scatter, used to measure the electron density of the tissues, and coherent scatter to obtain a measure of structure. Measurements of these parameters were made using a laboratory experimental set-up comprising an x-ray tube and HPGe detector. The breast tissue samples investigated comprise five different tissue classifications: adipose, malignancy, fibroadenoma, normal fibrous tissue and tissue that had undergone fibrocystic change. The coherent scatter spectra were analysed using a peak fitting routine, and a technique involving multivariate analysis was used to combine the peak fitted scatter profile spectra and the electron density values into a tissue classification model. The number of variables used in the model was refined by finding the sensitivity and specificity of each model and concentrating on differentiating between two tissues at a time. The best model that was formulated had a sensitivity of 54% and a specificity of 100%.

Evaluation of human muscle hardness after dynamic exercise with ultrasound real-time tissue elastography: A feasibility study

Energy Technology Data Exchange (ETDEWEB)

Yanagisawa, O., E-mail: o.yanagisawa@aoni.waseda.jp [Faculty of Sport Sciences, Waseda University, Tokorozawa, Saitama (Japan); Niitsu, M. [Department of Radiological Science, Faculty of Health Sciences, Tokyo Metropolitan University, Arakawa-ku, Tokyo (Japan); Kurihara, T. [Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga (Japan); Fukubayashi, T. [Faculty of Sport Sciences, Waseda University, Tokorozawa, Saitama (Japan)

2011-09-15

Aim: To assess the feasibility of ultrasound real-time tissue elastography (RTE) for measuring exercise-induced changes in muscle hardness and to compare the findings of RTE with those of a tissue hardness meter for semi-quantitative assessment of the hardness of exercised muscles. Materials and methods: Nine male participants performed an arm-curl exercise. RTE measurements were performed by manually applying repetitive compression with the transducer on the scan position before exercise, immediately after exercise, and at 30 min after exercise; strain ratios between muscle and a reference material (hydrogel) were calculated (muscle strain/material strain). A tissue hardness meter was also used to evaluate muscle hardness. The intraclass correlation coefficients (ICCs) for the three repeated measurements at each measurement time were calculated to evaluate the intra-observer reproducibility of each technique. Results: Immediately after exercise, the strain ratio and the value obtained using the tissue hardness meter significantly decreased (from 1.65 to 1.35) and increased (from 51.8 to 54.3), respectively. Both parameters returned to their pre-exercise value 30 min after exercise. The ICCs of the RTE (and the ICCs of the muscle hardness meter) were 0.971 (0.816) before exercise, 0.939 (0.776) immediately after exercise, and 0.959 (0.882) at 30 min after exercise. Conclusion: Similar to the muscle hardness meter, RTE revealed the exercise-induced changes of muscle hardness semi-quantitatively. The intra-observer reproducibility of RTE was very high at each measurement time. These findings suggest that RTE is a clinically useful technique for assessing hardness of specific exercised muscles.

Evaluation of human muscle hardness after dynamic exercise with ultrasound real-time tissue elastography: A feasibility study

International Nuclear Information System (INIS)

Yanagisawa, O.; Niitsu, M.; Kurihara, T.; Fukubayashi, T.

2011-01-01

Aim: To assess the feasibility of ultrasound real-time tissue elastography (RTE) for measuring exercise-induced changes in muscle hardness and to compare the findings of RTE with those of a tissue hardness meter for semi-quantitative assessment of the hardness of exercised muscles. Materials and methods: Nine male participants performed an arm-curl exercise. RTE measurements were performed by manually applying repetitive compression with the transducer on the scan position before exercise, immediately after exercise, and at 30 min after exercise; strain ratios between muscle and a reference material (hydrogel) were calculated (muscle strain/material strain). A tissue hardness meter was also used to evaluate muscle hardness. The intraclass correlation coefficients (ICCs) for the three repeated measurements at each measurement time were calculated to evaluate the intra-observer reproducibility of each technique. Results: Immediately after exercise, the strain ratio and the value obtained using the tissue hardness meter significantly decreased (from 1.65 to 1.35) and increased (from 51.8 to 54.3), respectively. Both parameters returned to their pre-exercise value 30 min after exercise. The ICCs of the RTE (and the ICCs of the muscle hardness meter) were 0.971 (0.816) before exercise, 0.939 (0.776) immediately after exercise, and 0.959 (0.882) at 30 min after exercise. Conclusion: Similar to the muscle hardness meter, RTE revealed the exercise-induced changes of muscle hardness semi-quantitatively. The intra-observer reproducibility of RTE was very high at each measurement time. These findings suggest that RTE is a clinically useful technique for assessing hardness of specific exercised muscles.

Measuring strain and rotation fields at the dislocation core in graphene

Science.gov (United States)

Bonilla, L. L.; Carpio, A.; Gong, C.; Warner, J. H.

2015-10-01

Strain fields, dislocations, and defects may be used to control electronic properties of graphene. By using advanced imaging techniques with high-resolution transmission electron microscopes, we have measured the strain and rotation fields about dislocations in monolayer graphene with single-atom sensitivity. These fields differ qualitatively from those given by conventional linear elasticity. However, atom positions calculated from two-dimensional (2D) discrete elasticity and three-dimensional discrete periodized Föppl-von Kármán equations (dpFvKEs) yield fields close to experiments when determined by geometric phase analysis. 2D theories produce symmetric fields whereas those from experiments exhibit asymmetries. Numerical solutions of dpFvKEs provide strain and rotation fields of dislocation dipoles and pairs that also exhibit asymmetries and, compared with experiments, may yield information on out-of-plane displacements of atoms. While discrete theories need to be solved numerically, analytical formulas for strains and rotation about dislocations can be obtained from 2D Mindlin's hyperstress theory. These formulas are very useful for fitting experimental data and provide a template to ascertain the importance of nonlinear and nonplanar effects. Measuring the parameters of this theory, we find two characteristic lengths between three and four times the lattice spacings that control dilatation and rotation about a dislocation. At larger distances from the dislocation core, the elastic fields decay to those of conventional elasticity. Our results may be relevant for strain engineering in graphene and other 2D materials of current interest.

Cardiac biplane strain imaging: initial in vivo experience

Science.gov (United States)

Lopata, R. G. P.; Nillesen, M. M.; Verrijp, C. N.; Singh, S. K.; Lammens, M. M. Y.; van der Laak, J. A. W. M.; van Wetten, H. B.; Thijssen, J. M.; Kapusta, L.; de Korte, C. L.

2010-02-01

In this study, first we propose a biplane strain imaging method using a commercial ultrasound system, yielding estimation of the strain in three orthogonal directions. Secondly, an animal model of a child's heart was introduced that is suitable to simulate congenital heart disease and was used to test the method in vivo. The proposed approach can serve as a framework to monitor the development of cardiac hypertrophy and fibrosis. A 2D strain estimation technique using radio frequency (RF) ultrasound data was applied. Biplane image acquisition was performed at a relatively low frame rate (dogs with an aortic stenosis. Initial results reveal the feasibility of measuring large radial, circumferential and longitudinal cumulative strain (up to 70%) at a frame rate of 100 Hz. Mean radial strain curves of a manually segmented region-of-interest in the infero-lateral wall show excellent correlation between the measured strain curves acquired in two perpendicular planes. Furthermore, the results show the feasibility and reproducibility of assessing radial, circumferential and longitudinal strains simultaneously. In this preliminary study, three beagles developed an elevated pressure gradient over the aortic valve (Δp: 100-200 mmHg) and myocardial hypertrophy. One dog did not develop any sign of hypertrophy (Δp = 20 mmHg). Initial strain (rate) results showed that the maximum strain (rate) decreased with increasing valvular stenosis (-50%), which is in accordance with previous studies. Histological findings corroborated these results and showed an increase in fibrotic tissue for the hearts with larger pressure gradients (100, 200 mmHg), as well as lower strain and strain rate values.

Transrectal real-time tissue elastography targeted biopsy coupled with peak strain index improves the detection of clinically important prostate cancer.

Science.gov (United States)

Ma, Qi; Yang, Dong-Rong; Xue, Bo-Xin; Wang, Cheng; Chen, Han-Bin; Dong, Yun; Wang, Cai-Shan; Shan, Yu-Xi

2017-07-01

The focus of the present study was to evaluate transrectal real-time tissue elastography (RTE)-targeted two-core biopsy coupled with peak strain index for the detection of prostate cancer (PCa) and to compare this method with 10-core systematic biopsy. A total of 141 patients were enrolled for evaluation. The diagnostic value of peak strain index was assessed using a receiver operating characteristic curve. The cancer detection rates of the two approaches and corresponding positive cores and Gleason score were compared. The cancer detection rate per core in the RTE-targeted biopsy (44%) was higher compared with that in systematic biopsy (30%). The peak strain index value of PCa was higher compared with that of the benign lesion. PCa was detected with the highest sensitivity (87.5%) and specificity (85.5%) using the threshold value of a peak strain index of ≥5.97 with an area under the curve value of 0.95. When the Gleason score was ≥7, RTE-targeted biopsy coupled with peak strain index detected 95.6% of PCa cases, but 84.4% were detected using systematic biopsy. Peak strain index as a quantitative parameter may improve the differentiation of PCa from benign lesions in the prostate peripheral zone. Transrectal RTE-targeted biopsy coupled with peak strain index may enhance the detection of clinically significant PCa, particularly when combined with systematic biopsy.

Experimental Evaluation of the Pathogenicity of Different Strains of Schistosoma mansoni

Directory of Open Access Journals (Sweden)

Antônio Aurélio Euzébio

2012-01-01

Full Text Available The pathogenesis of three different Schistosoma mansoni strains from the Brazilian states of Minas Gerais (BH strain and São Paulo (SJ and SD strains was evaluated in experimentally infected mice. Observations of the most severe clinical cases among local patients treated (SD strain in the city of Campinas (São Paulo, Brazil formed the basis of this study. Mice were used as definitive hosts and were infected with cercariae from Biomphalaria tenagophila (SJ and SD strains and Biomphalaria glabrata (BH strains. The parameters analyzed were as follows: number of S. mansoni eggs in mice feces; number of granulomas per tissue area in liver, spleen, lungs, pancreas, and ascending colon; measurements of hepatic and intestinal granulomas; number of adult worms; and measurements of trematode eggs. The comparison among the three strains indicated that the SD strain, isolated in Campinas, presented a higher worm recovery relative to the number of penetrating cercariae. In addition, when compared to the SJ and BH strains, the SD strain demonstrated similar pathogenicity to the BH strain, with a greater quantity of granulomas in the viscera, as well as larger granulomas and eggs. Furthermore, a greater quantity of trematode eggs was also shed in the feces.

Neutron-diffraction measurement of the evolution of strain for non-uniform plastic deformation

CERN Document Server

Rogge, R B; Boyce, D

2002-01-01

Neutrons are particularly adept for the validation of modeling predictions of stress and strain. In recent years, there has been a significant effort to model the evolution of both the macroscopic stresses and the intergranular stress during plastic deformation. These have had broad implications with regard to understanding the evolution of residual stress and to diffraction-based measurements of strain. Generally the modeling and associated measurements have been performed for simple uniaxial tension, leaving questions with regard to plastic deformation under multi-axial stress and non-uniform stress. Extensive measurements of the strain profile across a plastic hinge for each of a series of loading and unloading cycles to progressively higher degrees of plastic deformation are presented. These measurements are used to assess multiple-length-scale finite-element modeling (FEM) of the plastic hinge, in which the elements will range in size from single crystallites (as used in successful simulations of uniaxia...

Neutron-diffraction measurement of the evolution of strain for non-uniform plastic deformation

International Nuclear Information System (INIS)

Rogge, R.B.; Dawson, P.R.; Boyce, D.

2002-01-01

Neutrons are particularly adept for the validation of modeling predictions of stress and strain. In recent years, there has been a significant effort to model the evolution of both the macroscopic stresses and the intergranular stress during plastic deformation. These have had broad implications with regard to understanding the evolution of residual stress and to diffraction-based measurements of strain. Generally the modeling and associated measurements have been performed for simple uniaxial tension, leaving questions with regard to plastic deformation under multi-axial stress and non-uniform stress. Extensive measurements of the strain profile across a plastic hinge for each of a series of loading and unloading cycles to progressively higher degrees of plastic deformation are presented. These measurements are used to assess multiple-length-scale finite-element modeling (FEM) of the plastic hinge, in which the elements will range in size from single crystallites (as used in successful simulations of uniaxial tension) to macroscopic elements (as typically used in FEM simulations). (orig.)

Distributed strain measurement with polymer optical fibers integrated into multifunctional geotextiles

Science.gov (United States)

Liehr, Sascha; Lenke, Philipp; Krebber, Katerina; Seeger, Monika; Thiele, Elke; Metschies, Heike; Gebreselassie, Berhane; Münich, Johannes Christian; Stempniewski, Lothar

2008-04-01

Fiber optic sensors based on polymer optical fibers (POF) have the advantage of being very elastic and robust at the same time. Unlike silica fibers, standard PMMA POF fibers can be strained to more than 40% while fully maintaining their light guiding properties. We investigated POF as a distributed strain sensor by analysing the backscatter increase at the strained section using the optical time domain reflectometry (OTDR) technique. This sensing ability together with its high robustness and break-down strain makes POF well-suited for integration into technical textiles for structural health monitoring purposes. Within the European research project POLYTECT (Polyfunctional textiles against natural hazards) technical textiles with integrated POF sensors, among others sensors are being developed for online structural health monitoring of geotechnical structures. Mechanical deformation in slopes, dams, dikes, embankments and retrofitted masonry structures is to be detected before critical damage occurs. In this paper we present the POF strain sensor properties, reactions to disturbing influences as temperature and bends as well as the results of the different model tests we conducted within POLYTECT. We further show the potential of perfluorinated graded-index POF for distributed strain sensing with increased spatial resolution and measurement lengths.

Simultaneous measurement of dynamic strain and temperature distribution using high birefringence PANDA fiber Bragg grating

Science.gov (United States)

Zhu, Mengshi; Murayama, Hideaki

2017-04-01

New approach in simultaneous measurement of dynamic strain and temperature has been done by using a high birefringence PANDA fiber Bragg grating sensor. By this technique, we have succeeded in discriminating dynamic strain and temperature distribution at the sampling rate of 800 Hz and the spatial resolution of 1 mm. The dynamic distribution of strain and temperature were measured with the deviation of 5mm spatially. In addition, we have designed an experimental setup by which we can apply quantitative dynamic strain and temperature distribution to the fiber under testing without bounding it to a specimen.

Validity and interobserver agreement of lower extremity local tissue water measurements in healthy women using tissue dielectric constant

DEFF Research Database (Denmark)

Jensen, Mads R; Birkballe, Susanne; Nørregaard, Susan

2012-01-01

Tissue dielectric constant (TDC) measurement may become an important tool in the clinical evaluation of chronic lower extremity swelling in women; however, several factors are known to influence TDC measurements, and comparative data on healthy lower extremities are few.......Tissue dielectric constant (TDC) measurement may become an important tool in the clinical evaluation of chronic lower extremity swelling in women; however, several factors are known to influence TDC measurements, and comparative data on healthy lower extremities are few....

Measurement of strain and strain relaxation in free-standing Si membranes by convergent beam electron diffraction and finite element method

International Nuclear Information System (INIS)

Gao, H.; Ikeda, K.; Hata, S.; Nakashima, H.; Wang, D.; Nakashima, H.

2011-01-01

Bridge-shaped free-standing Si membranes (FSSM), strained by low-pressure (LP) Si x N y , plasma-enhanced (PE) Si x N y and Si x Ge 1-x stressors, were measured by convergent beam electron diffraction (CBED) and the finite element method (FEM). The results of CBED show that, while the strain along the length of the FSSM is compressive in an LPSi x N y /Si sample, those along the length of the FSSM are tensile in PESi x N y /Si and Si x Ge 1-x /Si samples. The average absolute values of strains are different in FSSM with LPSi x N y , PESi x N y and Si x Ge 1-x as stressors. The FEM was used to compensate the results of CBED taking into account the strain relaxation in transmission electron microscopy (TEM) sample preparation. The FEM results give the strain properties in three dimensions, and are in good agreement with the results of CBED. There is approximately no strain relaxation along the length of FSSM, and the elastic strains along the other two axes in FSSM are partially relaxed by thinning down for the preparation of TEM samples.

Planar strain analysis of liver undergoing microwave thermal ablation using x-ray CT.

Science.gov (United States)

Weiss, Noam; Goldberg, S Nahum; Nissenbaum, Yitzhak; Sosna, Jacob; Azhari, Haim

2015-01-01

To study the planar strain effects in liver during microwave (MW) thermal ablation as a means for tracking tissue expansion and contraction as a method for improving ablation monitoring. 1.4 mm circular metallic markers were inserted into 16 ex-vivo bovine fresh liver specimens, that were subsequently ablated (with the markers inside the specimen) by 40 W of microwave energy, for 1, 2, 3, 6, and 10 min. The markers were tracked during the ablation using an x-ray CT scanner. Images were acquired every 5-10 s enabling determination of the markers' coordinates over time. The 2D principal strains were calculated for triangles formed by subgroups of three markers, and their planar strain index, Ω, was plotted vs time. In addition, the radial distance of the markers from the antenna was measured at the end of each ablation. Subsequently, the tissue was sliced parallel to the imaged planes and the ablation zone was traced and digitized. The average ablation radius was then computed and compared to the radial distance. The planar strain, Ω(t), profile demonstrated an ascending pattern until reaching a maximum at about 180 s, with a mean peak value (Ω = 1.31 ± 0.04) indicating tissue expansion. Thereafter, Ω progressively declined over the remaining duration of the ablation treatment, indicating tissue contraction. Furthermore, when plotting the ablation size vs time and the markers' mean radial distance vs time, it was found that the two curves intercepted at a time corresponding to the time of peak planar strain. By detecting the point of maximal planar strain in tissues during MW application, it is possible to noninvasively identify the location of the ablation zone front. The fact that the liver tissue proximal to the ablated zone expands during the first part of the treatment and then contracts when the ablation front reaches it, may serve as an index for monitoring the thermal treatment.

Measurement of stress-strain behaviour of human hair fibres using optical techniques.

Science.gov (United States)

Lee, J; Kwon, H J

2013-06-01

Many studies have presented stress-strain relationship of human hair, but most of them have been based on an engineering stress-strain curve, which is not a true representation of stress-strain behaviour. In this study, a more accurate 'true' stress-strain curve of human hair was determined by applying optical techniques to the images of the hair deformed under tension. This was achieved by applying digital image cross-correlation (DIC) to 10× magnified images of hair fibres taken under increasing tension to estimate the strain increments. True strain was calculated by summation of the strain increments according to the theoretical definition of 'true' strain. The variation in diameter with the increase in longitudinal elongation was also measured from the 40× magnified images to estimate the Poisson's ratio and true stress. By combining the true strain and the true stress, a true stress-strain curve could be determined, which demonstrated much higher stress values than the conventional engineering stress-strain curve at the same degree of deformation. Four regions were identified in the true stress-strain relationship and empirical constitutive equations were proposed for each region. Theoretical analysis on the necking condition using the constitutive equations provided the insight into the failure mechanism of human hair. This analysis indicated that local thinning caused by necking does not occur in the hair fibres, but, rather, relatively uniform deformation takes place until final failure (fracture) eventually occurs. © 2012 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

Discussion on accuracy of weld residual stress measurement by neutron diffraction. Influence of strain free reference

International Nuclear Information System (INIS)

Suzuki, Hiroshi; Akita, Koichi

2012-01-01

It is required to evaluate a strain-free reference, α 0 , to perform accurate stress measurement using neutron diffraction. In this study, accuracy of neutron stress measurement was quantitatively discussed from α 0 evaluations on a dissimilar metal butt-weld between a type 304 austenitic stainless steel and an A533B low alloy ferritic steel. A strain-free standard specimen and a sliced specimen with 10 mm thickness taken from the dissimilar metal butt-weld were utilized. In the lattice constant evaluation using the standard specimen, average lattice constant derived from multiple hkl reflections was evaluated as the stress-free reference with cancelling out an intergranular strain. Comparing lattice constant distributions in each reflection with average lattice constant distribution in the standard specimen, αFe211 and γFe311 reflections were judged as a suitable reflection for neutron strain measurement to reduce intergranular strain effects. Residual stress distribution in the sliced specimen evaluated using α 0 measured here exhibited higher accuracy than that measured using strain gauges. On the other hand, α 0 distributions were evaluated using the sliced specimen under the plane-stress condition. Existence of slight longitudinal residual stresses near the weld center decreased accuracy of the α 0 evaluations, which means that it is required to optimize the thickness of the sliced specimen for accurate α 0 evaluation under plane strain condition. As a conclusion of this study, it was confirmed that procedures of accurate α 0 evaluation, optimization of the measurement condition, and multiple evaluations on the results play an important role to improve accuracy of the residual stress measurement using neutron diffraction. (author)

Elemental analysis of biological tissues of Dmdmdx/J and C57BL/6J mice strains investigated by neutron activation analysis

International Nuclear Information System (INIS)

Sabrina Metairon; Zamboni, C.B.; Suzuki, M.F.; Bueno Junior, C.R.; Sant'Anna, O.A.

2013-01-01

In order to understand in more details the alterations that Duchenne muscular dystrophy disease may cause in biological tissues (blood, tibia, quadriceps and heart), correlations matrixes of the Dmd mdx /J dystrophic mice as well as C57BL/6J (control group) were generated. These mice were obtained from Jackson Laboratory (Maine, USA) and bred at IPEN (Dmd mdx /J), and at Centro de Estudos do Genoma Humano (C57BL/6J), both research centers at Sao Paulo city. Elements of clinical and nutritional relevance (Br, Ca, Cl, K, Mg, Na and S) were investigated by neutron activation analysis. These measurements were performed using the nuclear reactor IEA-R1 (3.5-4.5 MW, pool type) at IPEN. Comparisons between concentrations and correlations in these biological tissues, of these strains, showed that a Ca and Mg in blood are altered for the dystrophic mice. A significant change in the heart of dystrophic mice was also observed suggesting that a constant monitoring is required. Moreover, these results may help the researchers to evaluate the efficiency of new treatments and to compare the advantages of different treatment approaches before performing tests in patients with muscular dystrophy. (author)

Stress Measurement around a Circular Role in a Cantilever Beam under Bending Moment Using Strain Gage and Reflective Photoelasticity

Energy Technology Data Exchange (ETDEWEB)

Baek, Tae Hyun; Park, Tae Geun; Yang, Min Bok [Kunsan National University, Gunsan (Korea, Republic of)

2006-10-15

It is necessary to study on the stress concentration experimentally, which is the main reason to avoid mechanical dilapidation and failure, when designing a mechanical structure. Stress concentration factor of a specimen of cantilever beam with a circular hole in the center was measured using both strain gage and photoelastic methods in this paper. In strain-gage measurement, three strain gages along the line near a hole of the specimen were installed and maximum strain was extrapolated from three measurements. In photoelastic measurement, two methods were employed. First, the Babinet-Soleil compensation method was used to measure the maximum strain. Secondly, photoelastic 4-step phase shilling method was applied to observe the strain distribution around the hole. Measurements obtained by different experiments were comparable within the range of experimental error

Stress Measurement around a Circular Role in a Cantilever Beam under Bending Moment Using Strain Gage and Reflective Photoelasticity

International Nuclear Information System (INIS)

Baek, Tae Hyun; Park, Tae Geun; Yang, Min Bok

2006-01-01

It is necessary to study on the stress concentration experimentally, which is the main reason to avoid mechanical dilapidation and failure, when designing a mechanical structure. Stress concentration factor of a specimen of cantilever beam with a circular hole in the center was measured using both strain gage and photoelastic methods in this paper. In strain-gage measurement, three strain gages along the line near a hole of the specimen were installed and maximum strain was extrapolated from three measurements. In photoelastic measurement, two methods were employed. First, the Babinet-Soleil compensation method was used to measure the maximum strain. Secondly, photoelastic 4-step phase shilling method was applied to observe the strain distribution around the hole. Measurements obtained by different experiments were comparable within the range of experimental error

Estimation of strain from piezoelectric effect and domain switching in morphotropic PZT by combined analysis of macroscopic strain measurements and synchrotron X-ray data

International Nuclear Information System (INIS)

Kungl, Hans; Theissmann, Ralf; Knapp, Michael; Baehtz, Carsten; Fuess, Hartmut; Wagner, Susanne; Fett, Theo; Hoffmann, Michael J.

2007-01-01

Morphotropic PZT ceramics are State of the art materials for ferroelectric actuators. Essential performance parameters for these materials are strain and hysteresis. On a microscopic scale the strain provided by an electric field is due to two different mechanisms. The piezoelectric effect causes an elongation of the unit cells, whereas domain switching changes their crystallographic orientation by aligning the polarization axis towards the field direction. A method is outlined to estimate the contribution of the two mechanisms to total strain by combining macroscopic strain measurements and X-ray diffraction (XRD) data. Results from macroscopic measurements of remanent and unipolar strain with the corresponding data on texture, derived from in situ synchrotron radiation XRD patterns, are analyzed and evaluated by a semi-empirical approach. The method was applied to six morphotropic, LaSr doped PZT materials of different Zr/Ti ratios. Results are discussed with respect to the differences between the materials

Strain measurements in a rotary engine housing

Science.gov (United States)

Lee, C. M.; Bond, T. H.; Addy, H. E.; Chun, K. S.; Lu, C. Y.

1989-01-01

The development of structural design tools for Rotary Combustion Engines (RCE) using Finite Element Modeling (FEM) requires knowledge about the response of engine materials to various service conditions. This paper describes experimental work that studied housing deformation as a result of thermal, pressure and mechanical loads. The measurement of thermal loads, clamping pressure, and deformation was accomplished by use of high-temperature strain gauges, thermocouples, and a high speed data acquisition system. FEM models for heat transfer stress analysis of the rotor housing will be verified and refined based on these experimental results.

Method for independent strain and temperature measurement in polymeric tensile test specimen using embedded FBG sensors

DEFF Research Database (Denmark)

Pereira, Gilmar Ferreira; McGugan, Malcolm; Mikkelsen, Lars Pilgaard

2016-01-01

to calculate independently the strain and temperature are presented in the article, together with a measurement resolution study. This multi-parameter measurement method was applied to an epoxy tensile specimen, tested in a unidirectional tensile test machine with a temperature controlled cabinet. A full......A novel method to obtain independent strain and temperature measurements using embedded Fibre Bragg Grating (FBG) in polymeric tensile test specimens is presented in this paper. The FBG strain and temperature cross-sensitivity was decoupled using two single mode FBG sensors, which were embedded...... of temperature, from 40 C to -10 C. The consistency of the expected theoretical results with the calibration procedure and the experimental validation shows that this proposed method is applicable to measure accurate strain and temperature in polymers during static or fatigue tensile testing. Two different...

Notch root strain measurement of WE43-T6 magnesium alloy using electronic speckle pattern interferometry

International Nuclear Information System (INIS)

Liew, H.L.; Ahmad, A.; Ramesh, S.; Purbolaksono, J.

2013-01-01

Highlights: • The use of ESPI for measuring total strains at the notch root of specimens. • Fine meshing in micron scale at the notch root regions. • The maximum elastic strain is shifted to be further away from the notch root tip. - Abstract: The notch root elasto-plastic strains of circumferentially grooved round specimen of cast magnesium WE43-T6 were experimentally measured using the electronic speckle pattern interferometry (ESPI) and numerically evaluated using the finite element analysis (FEA). The specimens have notch radii of 1.6 mm and 0.8 mm and an opening angle of 60°. The technique of ESPI showed its accuracy in measuring three-dimensional surface deformations on large negatively curved manifolds. The measured nominal stress for rupture is well beyond the ultimate strength, suggesting the existence of significant biaxial stress at the notch root region. The ESPI-based strains on the notch tips were shown to be in agreement with those evaluated by the FEA. The FEA also showed that the maximum elastic strain is shifted away from the notch root surface as the plastic strain is predominant

A pilot study using Tissue Velocity Ultrasound Imaging (TVI to assess muscle activity pattern in patients with chronic trapezius myalgia

Directory of Open Access Journals (Sweden)

Brodin Lars-Åke

2008-09-01

Full Text Available Abstract Background Different research techniques indicate alterations in muscle tissue and in neuromuscular control of aching muscles in patients with chronic localized pain. Ultrasound can be used for analysis of muscle tissue dynamics in clinical practice. Aim This study introduces a new muscle tissue sensitive ultrasound technique in order to provide a new methodology for providing a description of local muscle changes. This method is applied to investigate trapezius muscle tissue response – especially with respect to specific regional deformation and deformation rates – during concentric shoulder elevation in patients with chronic trapezius myalgia and healthy controls before and after pain provocation. Methods Patients with trapezius myalgia and healthy controls were analyzed using an ultrasound system equipped with tissue velocity imaging (TVI. The patients performed a standardized 3-cm concentric shoulder elevation before and after pain provocation/exercise at a standardized elevation tempo (30 bpm. A standardized region of interest (ROI, an ellipsis with a size that captures the upper and lower fascia of the trapezius muscle (4 cm width at rest, was placed in the first frame of the loop registration of the elevation. The ROI was re-anchored frame by frame following the same anatomical landmark in the basal fascia during all frames of the concentric phase. In cardiac measurement, tissue velocities are measured in the axial projection towards and against the probe where red colour represents shortening and red lengthening. In the case of measuring the trapezius muscle, tissue deformation measurements are made orthogonally, thus, indirectly. Based on the assumption of muscle volume incompressibility, blue represents tissue contraction and red relaxation. Within the ROI, two variables were calculated as a function of time: deformation and deformation rate. Hereafter, max, mean, and quadratic mean values (RMS of each variable were

Straining and wrinkling processes during turbulence-premixed flame interaction measured using temporally-resolved diagnostics

Energy Technology Data Exchange (ETDEWEB)

Steinberg, Adam M.; Driscoll, James F. [Department of Aerospace Engineering, The University of Michigan, Ann Arbor, MI 48109 (United States)

2009-12-15

The dynamical processes of flame surface straining and wrinkling that occur as turbulence interacts with a premixed flame were measured using cinema-stereoscopic PIV (CS-PIV) and orthogonal-plane cinema-stereoscopic PIV (OPCS-PIV). These diagnostics provided temporally resolved measurements of turbulence-flame interaction at frame rates of up to 3 kHz and spatial resolutions as small as 280{mu} m. Previous descriptions of flame straining and wrinkling have typically been derived based on a canonical interaction between a pair of counter-rotating vortices and a planar flame surface. However, it was found that this configuration did not properly represent real turbulence-flame interaction. Interactions resembling the canonical configuration were observed in less than 10% of the recorded frames. Instead, straining and wrinkling were generally caused more geometrically complex turbulence, consisting of large groups of structures that could be multiply curved and intertwined. The effect of the interaction was highly dependent on the interaction geometry. Furthermore, even when the turbulence did exist in the canonical geometry, the straining and wrinkling of the flame surface were not well characterized by the vortical structures. A new mechanistic description of the turbulence-flame interaction was therefore identified and confirmed by the measurements. In this description, flame surface straining is caused by coherent structures of fluid-dynamic strain-rate (strain-rate structures). The role of vortical structures is to curve existing flame surface, creating wrinkles. By simultaneously considering both forms of turbulent structure, turbulence-flame interactions in both the canonical configuration and more complex geometries could be understood. (author)

A deep learning approach to estimate chemically-treated collagenous tissue nonlinear anisotropic stress-strain responses from microscopy images.

Science.gov (United States)

Liang, Liang; Liu, Minliang; Sun, Wei

2017-11-01

Biological collagenous tissues comprised of networks of collagen fibers are suitable for a broad spectrum of medical applications owing to their attractive mechanical properties. In this study, we developed a noninvasive approach to estimate collagenous tissue elastic properties directly from microscopy images using Machine Learning (ML) techniques. Glutaraldehyde-treated bovine pericardium (GLBP) tissue, widely used in the fabrication of bioprosthetic heart valves and vascular patches, was chosen to develop a representative application. A Deep Learning model was designed and trained to process second harmonic generation (SHG) images of collagen networks in GLBP tissue samples, and directly predict the tissue elastic mechanical properties. The trained model is capable of identifying the overall tissue stiffness with a classification accuracy of 84%, and predicting the nonlinear anisotropic stress-strain curves with average regression errors of 0.021 and 0.031. Thus, this study demonstrates the feasibility and great potential of using the Deep Learning approach for fast and noninvasive assessment of collagenous tissue elastic properties from microstructural images. In this study, we developed, to our best knowledge, the first Deep Learning-based approach to estimate the elastic properties of collagenous tissues directly from noninvasive second harmonic generation images. The success of this study holds promise for the use of Machine Learning techniques to noninvasively and efficiently estimate the mechanical properties of many structure-based biological materials, and it also enables many potential applications such as serving as a quality control tool to select tissue for the manufacturing of medical devices (e.g. bioprosthetic heart valves). Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Myocardial left ventricular dysfunction in patients with systemic lupus erythematosus: new insights from tissue Doppler and strain imaging.

Science.gov (United States)

Buss, Sebastian J; Wolf, David; Korosoglou, Grigorios; Max, Regina; Weiss, Celine S; Fischer, Christian; Schellberg, Dieter; Zugck, Christian; Kuecherer, Helmut F; Lorenz, Hanns-Martin; Katus, Hugo A; Hardt, Stefan E; Hansen, Alexander

2010-01-01

Systemic lupus erythematosus (SLE) is associated with high cardiovascular morbidity and mortality. Cardiovascular involvement is frequently underestimated by routine imaging techniques. Our aim was to determine if new echocardiographic imaging modalities like tissue Doppler (TDI), strain rate (SRR), and strain (SRI) imaging detect abnormalities in left ventricular (LV) function in asymptomatic patients with SLE. Sixty-seven young patients with SLE (mean age 42 +/- 10 yrs) without typical symptoms or signs of heart failure or angina, and a matched healthy control group (n = 40), underwent standard transthoracic echocardiography, TDI, SRR, and SRI imaging of the LV as well as assessment of disease characteristics. Despite findings within the normal range on routine standard 2-dimensional echocardiography, SLE was associated with significantly impaired systolic and diastolic myocardial velocities of the LV measured by TDI [mean global TDI: systolic (s): 2.9 +/- 0.9 vs 3.9 +/- 0.7 cm/s, p < 0.05; early (e): 4.3 +/- 1.5 vs 6.3 +/- 1.3 cm/s, p < 0.05; late (a): 2.9 +/- 0.8 vs 3.4 +/- 0.8 cm/s, p < 0.05; values +/- SD); SRR (s: -0.8 +/- 0.1 vs -1.1 +/- 0.1 s(-1); e: 1.1 +/- 0.2 vs 1.6 +/- 0.3 s(-1); a: 0.7 +/- 0.1 vs 1.0 +/- 0.2 s(-1); all p < 0.05); and SR (-15.11 +/- 2.2% vs -19.7 +/- 1.9%; p < 0.05) compared to the control group. Further, elevated disease activity, measured with the ECLAM and the SLEDAI score, resulted in significantly lower values for LV longitudinal function measured by SRR and SR, but not by TDI. SLE is associated with a significant impairment of systolic and diastolic LV longitudinal function in patients without cardiac symptoms. New imaging modalities provide earlier insight into cardiovascular involvement in SLE and seem to be superior to standard echocardiography to detect subclinical myocardial disease.

Measurement of facial soft tissues thickness using 3D computed tomographic images

Energy Technology Data Exchange (ETDEWEB)

Jeong, Ho Gul; Kim, Kee Deog; Shin, Dong Won; Hu, Kyung Seok; Lee, Jae Bum; Park, Hyok; Park, Chang Seo [Yonsei Univ. Hospital, Seoul (Korea, Republic of); Han, Seung Ho [Catholic Univ. of Korea, Seoul (Korea, Republic of)

2006-03-15

To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side. The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. There were no statistically significant differences between the direct measurements and those using the 3D images(p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissue thickness more easily in forensic science and anthropology.

Measurement of facial soft tissues thickness using 3D computed tomographic images

International Nuclear Information System (INIS)

Jeong, Ho Gul; Kim, Kee Deog; Shin, Dong Won; Hu, Kyung Seok; Lee, Jae Bum; Park, Hyok; Park, Chang Seo; Han, Seung Ho

2006-01-01

To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side. The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. There were no statistically significant differences between the direct measurements and those using the 3D images(p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissue thickness more easily in forensic science and anthropology

Study of V-OTDR stability for dynamic strain measurement in piezoelectric vibration

Science.gov (United States)

Ren, Meiqi; Lu, Ping; Chen, Liang; Bao, Xiaoyi

2016-09-01

In a phase-sensitive optical-time domain reflectometry (Φ-OTDR) system, the challenge for dynamic strain measurement lies in large intensity fluctuations from trace to trace. The intensity fluctuation caused by stochastic characteristics of Rayleigh backscattering sets detection limit for the minimum strength of vibration measurement and causes the large measurement uncertainty. Thus, a trace-to-trace correlation coefficient is introduced to quantify intensity fluctuation of Φ-OTDR traces and stability of the sensor system theoretically and experimentally. A novel approach of measuring dynamic strain induced by various driving voltages of lead zirconate titanate (PZT) in Φ-OTDR is also demonstrated. Piezoelectric vibration signals are evaluated through analyzing peak values of fast Fourier transform spectra at the fundamental frequency and high-order harmonics based on Bessel functions. High trace-to-trace correlation coefficients varying from 0.824 to 0.967 among 100 measurements are obtained in experimental results, showing the good stability of our sensor system, as well as small uncertainty of measured peak values.

Biomimetic fetal rotation bioreactor for engineering bone tissues-Effect of cyclic strains on upregulation of osteogenic gene expression.

Science.gov (United States)

Ravichandran, Akhilandeshwari; Wen, Feng; Lim, Jing; Chong, Mark Seow Khoon; Chan, Jerry K Y; Teoh, Swee-Hin

2018-04-01

Cells respond to physiological mechanical stresses especially during early fetal development. Adopting a biomimetic approach, it is necessary to develop bioreactor systems to explore the effects of physiologically relevant mechanical strains and shear stresses for functional tissue growth and development. This study introduces a multimodal bioreactor system that allows application of cyclic compressive strains on premature bone grafts that are cultured under biaxial rotation (chamber rotation about 2 axes) conditions for bone tissue engineering. The bioreactor is integrated with sensors for dissolved oxygen levels and pH that allow real-time, non-invasive monitoring of the culture parameters. Mesenchymal stem cells-seeded polycaprolactone-β-tricalcium phosphate scaffolds were cultured in this bioreactor over 2 weeks in 4 different modes-static, cyclic compression, biaxial rotation, and multimodal (combination of cyclic compression and biaxial rotation). The multimodal culture resulted in 1.8-fold higher cellular proliferation in comparison with the static controls within the first week. Two weeks of culture in the multimodal bioreactor utilizing the combined effects of optimal fluid flow conditions and cyclic compression led to the upregulation of osteogenic genes alkaline phosphatase (3.2-fold), osteonectin (2.4-fold), osteocalcin (10-fold), and collagen type 1 α1 (2-fold) in comparison with static cultures. We report for the first time, the independent and combined effects of mechanical stimulation and biaxial rotation for bone tissue engineering using a bioreactor platform with non-invasive sensing modalities. The demonstrated results show leaning towards the futuristic vision of using a physiologically relevant bioreactor system for generation of autologous bone grafts for clinical implantation. Copyright © 2018 John Wiley & Sons, Ltd.

Strain measurements of nuclear power plant steam generator antiseismic supports

International Nuclear Information System (INIS)

Kulichevsky, R.

1997-01-01

The nuclear power plants steam generators have different types of structural supports. One of these types are the antiseismic supports, which are intended to be under stress only if a seismic event takes place. Nevertheless, the antiseismic supports lugs, that are welded to the steam generator vessel, are subjected to thermal fatigue because of the temperature cycles related with the shut down and start up operations performed during the life of the nuclear power plant. In order to evaluate the stresses that the lugs are subjected to, several strain gages were welded on two supports lugs, positioned at two heights of one of the Embalse nuclear power plant steam generators. In this paper, the instrumentation used and the strain measurements obtained during two start up operations are presented. The influence of the plant start up operation parameters on the lugs strain evolution is also analyzed. (author) [es

Measurement of strain and strain relaxation in free-standing Si membranes by convergent beam electron diffraction and finite element method

Energy Technology Data Exchange (ETDEWEB)

Gao, H., E-mail: hongye18@mm.kyushu-u.ac.jp [Faculty of Engineering Sciences, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka 816-8580 (Japan); Ikeda, K.; Hata, S.; Nakashima, H. [Faculty of Engineering Sciences, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka 816-8580 (Japan); Wang, D.; Nakashima, H. [Art, Science and Technology Center for Cooperative Research, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka 816-8580 (Japan)

2011-04-15

Bridge-shaped free-standing Si membranes (FSSM), strained by low-pressure (LP) Si{sub x}N{sub y}, plasma-enhanced (PE) Si{sub x}N{sub y} and Si{sub x}Ge{sub 1-x} stressors, were measured by convergent beam electron diffraction (CBED) and the finite element method (FEM). The results of CBED show that, while the strain along the length of the FSSM is compressive in an LPSi{sub x}N{sub y}/Si sample, those along the length of the FSSM are tensile in PESi{sub x}N{sub y}/Si and Si{sub x}Ge{sub 1-x}/Si samples. The average absolute values of strains are different in FSSM with LPSi{sub x}N{sub y}, PESi{sub x}N{sub y} and Si{sub x}Ge{sub 1-x} as stressors. The FEM was used to compensate the results of CBED taking into account the strain relaxation in transmission electron microscopy (TEM) sample preparation. The FEM results give the strain properties in three dimensions, and are in good agreement with the results of CBED. There is approximately no strain relaxation along the length of FSSM, and the elastic strains along the other two axes in FSSM are partially relaxed by thinning down for the preparation of TEM samples.

Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.

Science.gov (United States)

Nordberg, Rachel C; Bodle, Josie C; Loboa, Elizabeth G

2018-01-01

It is critical that human adipose stem cell (hASC) tissue-engineering therapies possess appropriate mechanical properties in order to restore function of the load bearing tissues of the musculoskeletal system. In an effort to elucidate the hASC response to mechanical stimulation and develop mechanically robust tissue engineered constructs, recent research has utilized a variety of mechanical loading paradigms including cyclic tensile strain, cyclic hydrostatic pressure, and mechanical unloading in simulated microgravity. This chapter describes methods for applying these mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system.

Strain measurements using Fiber Bragg Grating sensors in Structural Health Monitoring

Directory of Open Access Journals (Sweden)

Daniela ENCIU

2017-06-01

Full Text Available The paper presents some results obtained within a project of the “NUCLEU” Program financed by the Ministry of Research and Innovation-ANCS. The project supposes, among others, the design and the realization of a demonstrator for strain and stress measurements made with Fiber Bragg Gratings optical sensors. The paper details the construction of the demonstrator. The strain measurements induced in a cantilevered aluminum plate are compared with the analytical values provided by a mathematical model, and with the numerical values obtained by FEM analysis. The consistency of these comparative data indicates the achievement within the project of a level of competence necessary for later use of FBG sensors in the applicative researches involving the aerospace structures monitoring.

Measurement of internal forces in superconducting accelerator magnets with strain gauge transducers

International Nuclear Information System (INIS)

Goodzeit, C.L.; Anerella, M.D.; Ganetis, G.L.

1988-01-01

An improved method has been developed for the measurement of internal forces in superconducting accelerator magnets, in particular the compressive stresses in coils and the end restraint forces on the coils. The transducers have been designed to provide improved sensitivity to purely mechanical strain by using bending mode deflections for sensing the applied loads. Strain gauge resistance measurements are made with a new system that eliminates sources of errors due to spurious resistance changes in interconnecting wiring and solder joints. The design of the transducers and their measurement system is presented along with a discussion of the method of compensation for thermal and magnetic effects, methods of calibration with typical calibration data, and measured effect in actual magnets of the thermal stress changes from cooldown and the Lorentz forces during magnet excitation. 13 figs., 1 tab

Elements determination of clinical relevance in biological tissues Dmdmdx/J dystrophic mice strains investigated by NAA

International Nuclear Information System (INIS)

Metairon, Sabrina

2012-01-01

In this work the determination of chemistry elements in biological tissues (whole blood, bones and organs) of dystrophic mice, used as animal model of Duchenne Muscular Dystrophy (DMD), was performed using analytical nuclear technique. The aim of this work was to determine reference values of elements of clinical (Ca, Cl, K, Mg, Na) and nutritional (Br and S) relevance in whole blood, tibia, quadriceps and hearts from Dmdmdx/J (10 males and 10 females) dystrophic mice and C57BL/6J (10 males) control group mice, using Neutron Activation Analysis technique (NAA). To show in more details the alterations that this disease may cause in these biological tissues, correlations matrixes of the DMD mdx /J mouse strain were generated and compared with C57BL/6J control group. For this study 119 samples of biological tissue were irradiated in the IEA-R1 nuclear reactor at IPEN (Sao Paulo, Brazil). The concentrations of these elements in biological tissues of Dmd mdx /J and C57B/6J mice are the first indicative interval for reference values. Moreover, the alteration in some correlation coefficients data among the elements in the health status and in the diseased status indicates a connection between these elements in whole blood, tibia, quadriceps and heart. These results may help the researchers to evaluate the efficiency of new treatments and to compare the advantages of different treatment approaches before performing tests in patients with muscular dystrophy. (author)

Instrumentation and method for measuring NIR light absorbed in tissue during MR imaging in medical NIRS measurements

Science.gov (United States)

Myllylä, Teemu S.; Sorvoja, Hannu S. S.; Nikkinen, Juha; Tervonen, Osmo; Kiviniemi, Vesa; Myllylä, Risto A.

2011-07-01

Our goal is to provide a cost-effective method for examining human tissue, particularly the brain, by the simultaneous use of functional magnetic resonance imaging (fMRI) and near-infrared spectroscopy (NIRS). Due to its compatibility requirements, MRI poses a demanding challenge for NIRS measurements. This paper focuses particularly on presenting the instrumentation and a method for the non-invasive measurement of NIR light absorbed in human tissue during MR imaging. One practical method to avoid disturbances in MR imaging involves using long fibre bundles to enable conducting the measurements at some distance from the MRI scanner. This setup serves in fact a dual purpose, since also the NIRS device will be less disturbed by the MRI scanner. However, measurements based on long fibre bundles suffer from light attenuation. Furthermore, because one of our primary goals was to make the measuring method as cost-effective as possible, we used high-power light emitting diodes instead of more expensive lasers. The use of LEDs, however, limits the maximum output power which can be extracted to illuminate the tissue. To meet these requirements, we improved methods of emitting light sufficiently deep into tissue. We also show how to measure NIR light of a very small power level that scatters from the tissue in the MRI environment, which is characterized by strong electromagnetic interference. In this paper, we present the implemented instrumentation and measuring method and report on test measurements conducted during MRI scanning. These measurements were performed in MRI operating rooms housing 1.5 Tesla-strength closed MRI scanners (manufactured by GE) in the Dept. of Diagnostic Radiology at the Oulu University Hospital.

Photoplethysmographic sensors for perfusion measurements in spinal cord tissue

Energy Technology Data Exchange (ETDEWEB)

Phillips, J P; Kyriacou, P A, E-mail: Justin.Phillips.1@city.ac.uk [School of Engineering and Mathematical Sciences, City University London, EC1V 0HB (United Kingdom)

2011-08-17

Sensors for recording photoplethysmographic signals from the nervous tissue of the spinal cord are described. The purpose of these sensors is to establish whether perfusion is compromised in various states of injury which occur in certain animal models of spinal cord injury, for example compression injury. Various measures of perfusion are applicable such as the amplitude of the photoplethysmograph signal and the oxygen saturation, measured using a dual wavelength configuration. Signals are usually compared to baseline measurements made in uninjured subjects. This paper describes two types of probe, one based on optical fibres, and one in which optotes are placed in direct contact with the tissue surface. Results from a study based on a compression model utilising a fibreoptic sensor are presented.

Triaxial extensometer for volumetric strain measurement in a hydro-compression loading test for foam materials

International Nuclear Information System (INIS)

Feng, Bo; Xu, Ming-long; Zhao, Tian-fei; Zhang, Zhi-jun; Lu, Tian-jian

2010-01-01

A new strain gauge-based triaxial extensometer (radial extensometers x, y and axial extensometer z) is presented to improve the volumetric strain measurement in a hydro-compression loading test for foam materials. By the triaxial extensometer, triaxial deformations of the foam specimen can be measured directly, from which the volumetric strain is determined. Sensitivities of the triaxial extensometer are predicted using a finite-element model, and verified through experimental calibrations. The axial extensometer is validated by conducting a uniaxial compression test in aluminium foam and comparing deformation measured by the axial extensometer to that by the advanced optical 3D deformation analysis system ARAMIS; the result from the axial extensometer agrees well with that from ARAMIS. A new modus of two-wire measurement and transmission in a hydrostatic environment is developed to avoid the punching and lead sealing techniques on the pressure vessel for the hydro-compression test. The effect of hydrostatic pressure on the triaxial extensometer is determined through an experimental test. An application in an aluminium foam hydrostatic compression test shows that the triaxial extensometer is effective for volumetric strain measurement in a hydro-compression loading test for foam materials

Measurement of strain distribution in bonded joints by fiber Bragg gratings

Science.gov (United States)

Guemes, J. Alfredo; Diaz-Carrillo, Sebastian; Menendez, Jose M.

1998-07-01

Due to the small dimensions of the adhesive layer, the high non-uniformity of the strain field and the non linear elastic behavior of the adhesive material, the strain distribution at an adhesive joint can be predicted by FEM, but can not be experimentally obtained with classical approaches; only non standard procedures like Moire interferometry, or special artifacts like KGR extensometers may afford some insights on the behavior of the adhesive. Due to their small size, ensuring low perturbation of the strain field, and their innate ability to measure strain and strain gradient along the sensor, fiber Bragg gratings offer a good opportunity to solve this problem, and it is a good example of situations that may benefit from these new sensors. Fiber Bragg gratings may be placed or at the interface, within the adhesive layer, or embedded at the adherents, if these were made of composite material. Tests may be run at different temperatures, changing the adhesive characteristics from brittle to pseudoplastic without additional difficulties. When loading the joint, the strain field is obtained by analyzing the distorted spectrum of the reflected light pulse; the algorithm for doing it has already been published. A comparison with theoretical results is done, and the validity and utility of these sensors for this and similar applications is demonstrated.

Intermittent straining accelerates the development of tissue properties in engineered heart valve tissue

NARCIS (Netherlands)

Rubbens, M.P.; Mol, A.; Boerboom, R.A.; Bank, R.A.; Baaijens, F.P.T.; Bouten, C.V.C.

2009-01-01

Tissue-engineered heart valves lack sufficient amounts of functionally organized structures and consequently do not meet in vivo mechanical demands. To optimize tissue architecture and hence improve mechanical properties, various in vitro mechanical conditioning protocols have been proposed, of

A fiber-optic strain measurement and quench localization system for use in superconducting accelerator dipole magnets

NARCIS (Netherlands)

van Oort, J.M.; Scanlan, Ronald M.; ten Kate, Herman H.J.

1995-01-01

A novel fiber-optic measurement system for superconducting accelerator magnets is described. The principal component is an extrinsic Fabry-Perot interferometer to determine localized strain and stress in coil windings. The system can be used either as a sensitive relative strain measurement system

Reliability of in vivo measurements of the dielectric properties of anisotropic tissue: a simulative study

International Nuclear Information System (INIS)

Huo Xuyang; Shi Xuetao; You Fusheng; Fu Feng; Liu Ruigang; Tang Chi; Dong Xiuzhen; Lu Qiang

2013-01-01

A simulative study was performed to measure the dielectric properties of anisotropic tissue using several in vivo and in vitro probes. COMSOL Multiphysics was selected to carry out the simulation. Five traditional probes and a newly designed probe were used in this study. One of these probes was an in vitro measurement probe and the other five were in vivo. The simulations were performed in terms of the minimal tissue volume for in vivo measurements, the calibration of a probe constant, the measurement performed on isotropic tissue and the measurement performed on anisotropic tissue. Results showed that the in vitro probe can be used to measure the in-cell dielectric properties of isotropic and anisotropic tissues. When measured with the five in vivo probes, the dielectric properties of isotropic tissue were all measured accurately. For the measurements performed on anisotropic tissue, large errors were observed when the four traditional in vivo probes were used, but only a small error was observed when the new in vivo probe was used. This newly designed five-electrode in vivo probe may indicate the dielectric properties of anisotropic tissue more accurately than these four traditional in vivo probes. (paper)

Stretching positions for the coracohumeral ligament: Strain measurement during passive motion using fresh/frozen cadaver shoulders

Directory of Open Access Journals (Sweden)

Izumi Tomoki

2011-01-01

Full Text Available Abstract Background Contracture of the coracohumeral ligament is reported to restrict external rotation of the shoulder with arm at the side and restrict posterior-inferior shift of the humeral head. The contracture is supposed to restrict range of motion of the glenohumeral joint. Methods To obtain stretching position of the coracohumeral ligament, strain on the ligament was measured at the superficial fibers of the ligament using 9 fresh/frozen cadaver shoulders. By sequential measurement using a strain gauge, the ligament strain was measured from reference length (L0. Shoulder positions were determined using a 3 Space Tracker System. Through a combination of previously reported coracohumeral stretching positions and those observed in preliminary measurement, ligament strain were measured by passive external rotation from 10° internal rotation, by adding each 10° external rotation, to maximal external rotation. Results Stretching positions in which significantly larger strain were obtained compared to the L0 values were 0° elevation in scapula plane with 40°, 50° and maximum external rotation (5.68%, 7.2%, 7.87%, 30° extension with 50°, maximum external rotation (4.20%, 4.79%, and 30° extension + adduction with 30°, 40°, 50° and maximum external rotation (4.09%, 4.67%, 4.78%, 5.05%(P Conclusions Significant strain of the coracohumeral ligament will be achieved by passive external rotation at lower shoulder elevations, extension, and extension with adduction.

Strain measurements at the HDR-pipe-system under LOCA-load: Effects on elbows and displaced weldings

International Nuclear Information System (INIS)

Hunger, H.

1985-01-01

This paper characterizes some effects which have been detected during strain gauge measurements on a test piping with feed water check valve oscillating under blowdown-load. The ovalization of a pipe elbow subjected to in-plane-bending affects the connected straight pipe; this is shown by means of circumferential stresses. Very high LOCA-load produces plastic strain and changes the pipe dynamics. Artificial displaced welds increase the local strain but no defects have occurred. One example compares stresses from measurement and post-calculation. Moreover there are given some remarks on the optimization of the comparison of measurement and calculation. (orig.)

Cardiac biplane strain imaging: initial in vivo experience

Energy Technology Data Exchange (ETDEWEB)

Lopata, R G P; Nillesen, M M; Thijssen, J M; De Korte, C L [Clinical Physics Laboratory, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Verrijp, C N; Lammens, M M Y; Van der Laak, J A W M [Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Singh, S K; Van Wetten, H B [Department of Cardiothoracic Surgery, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Kapusta, L [Pediatric Cardiology, Department of Pediatrics, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands)], E-mail: R.Lopata@cukz.umcn.nl

2010-02-21

In this study, first we propose a biplane strain imaging method using a commercial ultrasound system, yielding estimation of the strain in three orthogonal directions. Secondly, an animal model of a child's heart was introduced that is suitable to simulate congenital heart disease and was used to test the method in vivo. The proposed approach can serve as a framework to monitor the development of cardiac hypertrophy and fibrosis. A 2D strain estimation technique using radio frequency (RF) ultrasound data was applied. Biplane image acquisition was performed at a relatively low frame rate (<100 Hz) using a commercial platform with an RF interface. For testing the method in vivo, biplane image sequences of the heart were recorded during the cardiac cycle in four dogs with an aortic stenosis. Initial results reveal the feasibility of measuring large radial, circumferential and longitudinal cumulative strain (up to 70%) at a frame rate of 100 Hz. Mean radial strain curves of a manually segmented region-of-interest in the infero-lateral wall show excellent correlation between the measured strain curves acquired in two perpendicular planes. Furthermore, the results show the feasibility and reproducibility of assessing radial, circumferential and longitudinal strains simultaneously. In this preliminary study, three beagles developed an elevated pressure gradient over the aortic valve ({delta}p: 100-200 mmHg) and myocardial hypertrophy. One dog did not develop any sign of hypertrophy ({delta}p = 20 mmHg). Initial strain (rate) results showed that the maximum strain (rate) decreased with increasing valvular stenosis (-50%), which is in accordance with previous studies. Histological findings corroborated these results and showed an increase in fibrotic tissue for the hearts with larger pressure gradients (100, 200 mmHg), as well as lower strain and strain rate values.

Cardiac biplane strain imaging: initial in vivo experience

International Nuclear Information System (INIS)

Lopata, R G P; Nillesen, M M; Thijssen, J M; De Korte, C L; Verrijp, C N; Lammens, M M Y; Van der Laak, J A W M; Singh, S K; Van Wetten, H B; Kapusta, L

2010-01-01

In this study, first we propose a biplane strain imaging method using a commercial ultrasound system, yielding estimation of the strain in three orthogonal directions. Secondly, an animal model of a child's heart was introduced that is suitable to simulate congenital heart disease and was used to test the method in vivo. The proposed approach can serve as a framework to monitor the development of cardiac hypertrophy and fibrosis. A 2D strain estimation technique using radio frequency (RF) ultrasound data was applied. Biplane image acquisition was performed at a relatively low frame rate (<100 Hz) using a commercial platform with an RF interface. For testing the method in vivo, biplane image sequences of the heart were recorded during the cardiac cycle in four dogs with an aortic stenosis. Initial results reveal the feasibility of measuring large radial, circumferential and longitudinal cumulative strain (up to 70%) at a frame rate of 100 Hz. Mean radial strain curves of a manually segmented region-of-interest in the infero-lateral wall show excellent correlation between the measured strain curves acquired in two perpendicular planes. Furthermore, the results show the feasibility and reproducibility of assessing radial, circumferential and longitudinal strains simultaneously. In this preliminary study, three beagles developed an elevated pressure gradient over the aortic valve (Δp: 100-200 mmHg) and myocardial hypertrophy. One dog did not develop any sign of hypertrophy (Δp = 20 mmHg). Initial strain (rate) results showed that the maximum strain (rate) decreased with increasing valvular stenosis (-50%), which is in accordance with previous studies. Histological findings corroborated these results and showed an increase in fibrotic tissue for the hearts with larger pressure gradients (100, 200 mmHg), as well as lower strain and strain rate values.

Heat capacity measurements of Sr{sub 2}RuO{sub 4} under uni-axial strain

Energy Technology Data Exchange (ETDEWEB)

Li, You-sheng; Mackenzie, Andrew [Max Planck Institute for Chemical Physics of Solids, Dresden (Germany); University of St. Andrews, School of Physics and Astronomy (United Kingdom); Gibbs, Alexandra [Max Planck Institute for Solid State Research, Stuttgart (Germany); Hicks, Clifford [Max Planck Institute for Chemical Physics of Solids, Dresden (Germany); Nicklas, Michael [University of St. Andrews, School of Physics and Astronomy (United Kingdom)

2016-07-01

One of the most-discussed possible pairing symmetries of Sr{sub 2}RuO{sub 4} is p{sub x} ± ip{sub y}. By applying strain along left angle 100 right angle -direction, the degeneracy of the p{sub x} and p{sub y} components is lifted, and thus there should be two critical temperatures (T{sub c}). Hicks et al. have observed an increase of T{sub c} of Sr{sub 2}RuO{sub 4} under both compressive and tensile strains, by measuring the susceptibility, which is sensitive only to the first transition. Their results also indicate, indirectly, that any splitting of T{sub c}s might be small. For a direct test of possible splitting, we measure the heat capacity of Sr{sub 2}RuO{sub 4} under strain. To do so, we are developing an approach to measure heat capacity under non-adiabatic conditions. We have observed an increase of T{sub c} under compressive strain. This is the first thermodynamic evidence of the strain-induced increase in T{sub c} of Sr{sub 2}RuO{sub 4}.

Measurement of mean rotation and strain-rate tensors by using stereoscopic PIV

DEFF Research Database (Denmark)

Özcan, Oktay; Meyer, Knud Erik; Larsen, Poul Scheel

2005-01-01

A technique is described for measuring the mean velocity gradient (rate-of-displacement) tensor by using a conventional stereoscopic particle image velocimetry (SPIV) system. Planar measurement of the mean vorticity vector, rate-of-rotation and rate-of-strain tensors and the production of turbule...

A novel semi-quantitative method for measuring tissue bleeding.

Science.gov (United States)

Vukcevic, G; Volarevic, V; Raicevic, S; Tanaskovic, I; Milicic, B; Vulovic, T; Arsenijevic, S

2014-03-01

In this study, we describe a new semi-quantitative method for measuring the extent of bleeding in pathohistological tissue samples. To test our novel method, we recruited 120 female patients in their first trimester of pregnancy and divided them into three groups of 40. Group I was the control group, in which no dilation was applied. Group II was an experimental group, in which dilation was performed using classical mechanical dilators. Group III was also an experimental group, in which dilation was performed using a hydraulic dilator. Tissue samples were taken from the patients' cervical canals using a Novak's probe via energetic single-step curettage prior to any dilation in Group I and after dilation in Groups II and III. After the tissue samples were prepared, light microscopy was used to obtain microphotographs at 100x magnification. The surfaces affected by bleeding were measured in the microphotographs using the Autodesk AutoCAD 2009 program and its "polylines" function. The lines were used to mark the area around the entire sample (marked A) and to create "polyline" areas around each bleeding area on the sample (marked B). The percentage of the total area affected by bleeding was calculated using the formula: N = Bt x 100 / At where N is the percentage (%) of the tissue sample surface affected by bleeding, At (A total) is the sum of the surfaces of all of the tissue samples and Bt (B total) is the sum of all the surfaces affected by bleeding in all of the tissue samples. This novel semi-quantitative method utilizes the Autodesk AutoCAD 2009 program, which is simple to use and widely available, thereby offering a new, objective and precise approach to estimate the extent of bleeding in tissue samples.

Longitudinally Jointed Edge-wise Compression Honeycomb Composite Sandwich Coupon Testing and FE Analysis: Three Methods of Strain Measurement, and Comparison

Science.gov (United States)

Farrokh, Babak; AbdulRahim, Nur Aida; Segal, Ken; Fan, Terry; Jones, Justin; Hodges, Ken; Mashni, Noah; Garg, Naman; Sang, Alex; Gifford, Dawn;

Simultaneous measurement of temperature and strain using a phase-shifted fiber Bragg grating inscribed by femtosecond laser

Science.gov (United States)

Jiang, Yajun; Liu, Chi; Li, Dong; Yang, Dexing; Zhao, Jianlin

2018-04-01

A novel method for simultaneous measurement of temperature and strain using a single phase-shifted fiber Bragg grating (PS-FBG) is proposed. The PS-FBG is produced by exposing the fusion-spliced fiber with a femtosecond laser and uniform phase mask. Due to the non-uniform structure and strain distribution in the fusion-spliced region, the phase-shift changes with different responses during increases to the temperature and strain; by measuring the central wavelengths and the loss difference of two transmission dips, temperature and strain can be determined simultaneously. The resolutions of this particular sensor in measuring temperature and strain are estimated to be  ±1.5 °C and  ±12.2 µɛ in a range from  -50 °C to 150 °C and from 0 µɛ to 2070 µɛ.

Scintigraphic measurements of gastric emptying corrected for differences in tissue attenuation

Energy Technology Data Exchange (ETDEWEB)

Lauritzen, J.B.; Hoejgaard, L.; Uhrenholdt, A. (Copenhagen Univ. (Denmark). Hvidovre Hospital)

1983-10-01

In order to evaluate the importance of variations in tissue attenuation in scintigraphic measurements of gastric emptying, both in vivo and in vitro measurements of count rates from an encapsulated sup(99m)Tc dose were performed in different parts of the stomach. The obtained individual tissue correction factors were applied in the calculation of gastric emptying rates by gamma camera in healthy volunteers. The results showed that the anterior gamma camera scan without correction for differences in tissue attenuation underestimated the gastric emptying rate by 11% if the results were expressed as percentage meal emptied over 60 minutes.

Measurement of human normal tissue and tumour responses

International Nuclear Information System (INIS)

Ross, G.; Yarnold, J.R.

1988-01-01

The scarcity of quantitative measures of normal tissue damage and tumour response in patients undergoing radiotherapy is an obstacle to the clinical evaluation of new treatment strategies. Retrospective studies of complications in critical normal tissues taught important lessons in the past concerning the potential dangers of hypofractionation. However, it is unethical to use serious complications as planned end-points in prospective studies. This paper reviews the desirable characteristics of clinical end-points required to compare alternative treatments employing radiotherapy, with emphasis on simple scales applied by clinicians or even the patients themselves

In-situ measurement of the strain distribution in a tensile specimen by using a digital speckle pattern interferometer

International Nuclear Information System (INIS)

Park, Seung-Kyu; Baik, Sung-Hoon; Cha, Hyung-Ki; Kim, Young-Suk; Cheong, Yong-Moo

2010-01-01

Less sensitivity to environmental vibrations is essential for industrial applications of a digital speckle pattern interferometer (DSPI) to measure micro deformations. In this paper, a robust DSPI using single fringe to mechanical vibrations is designed for measuring the strain distribution of a tensile specimen. This system adopts a noise-immune signal processing algorithm to acquire a 3D strain distribution image. To acquire an accurate strain distribution for a tensile-specimen, locally-averaged and directionally-oriented filters operating in the frequency domain are used. This system uses a path-independent least-squares phase-unwrapping algorithm to acquire the 3D shape of the strain distribution. As for the initial experiments to measure the strain distribution of a tensile specimen in a vibration field, this system demonstrated a feasibility for industrial applications by providing reliable strain data.

Twin-Core Fiber-Based Mach Zehnder Interferometer for Simultaneous Measurement of Strain and Temperature

Science.gov (United States)

Kowal, Dominik; Urbanczyk, Waclaw; Mergo, Pawel

2018-01-01

In this paper we present an all-fiber interferometric sensor for the simultaneous measurement of strain and temperature. It is composed of a specially fabricated twin-core fiber spliced between two pieces of a single-mode fiber. Due to the refractive index difference between the two cores in a twin-core fiber, a differential interference pattern is produced at the sensor output. The phase response of the interferometer to strain and temperature is measured in the 850–1250 nm spectral range, showing zero sensitivity to strain at 1000 nm. Due to the significant difference in sensitivities to both parameters, our interferometer is suitable for two-parameter sensing. The simultaneous response of the interferometer to strain and temperature was studied using the two-wavelength interrogation method and a novel approach based on the spectral fitting of the differential phase response. As the latter technique uses all the gathered spectral information, it is more reliable and yields the results with better accuracy. PMID:29558386

Strain analysis of I-c(epsilon) characteristic of YBCO coated conductor measured by a Walters spring

OpenAIRE

Sugano, M; Choi, S; Miyazoe, A; Miyamatsu, K; Ando, T; Itoh, K; Kiyoshi, T; Wada, H; Selvamanickam, V

2008-01-01

lc-strain characteristic of YBCO coated conductor was measured using a Walters spring (WASP). In this technique, additional bending and thermal strains induced to the YBCO layer should be considered. In order to produce different initial bending strain to the YBCO layer, the conductor was wound around the springs with different diameters and in the different bending directions. The clear evidence was obtained that -strain curves using a WASP strongly depend on the initial bending strain state...

Early detection of left ventricular dysfunction in asymptomatic diabetic patient using strain and strain rate echocardiographic imaging

Directory of Open Access Journals (Sweden)

Rania Gaber

2014-03-01

Conclusion: Type 2 diabetes mellitus deteriorate both LV systolic and diastolic performance. Strain and strain rate by tissue Doppler Imaging is superior to conventional Doppler in early detection and evaluation of systolic and diastolic dysfunction in type 2 diabetic patients.

Development of serial measurement system for three-dimensional stress determination by over-coring the strains on borehole wall

International Nuclear Information System (INIS)

Itamoto, Masaharu; Kuwabara, Kazumichi; Tanno, Takeo; Nakayama, Yoshiki; Mizuta, Yoshiaki

2007-01-01

In order to determine the three-dimensional stress state in serial order, the authors developed the serial measurement system for three-dimensional stress determination by over-coring the strains on the borehole wall. The serial stress measurements give the value of the stresses with high accuracy and bring the regional stress variations. In this paper, the authors describe the studies through FEM analysis on the effect of over-coring diameter, the influence of strain gauge length and the behavior of strain on the borehole wall, induced by biaxial external loading. We developed the multi-strain gauge mounted packer and examined it by measuring the strains on the borehole wall through biaxial loading test. The Laboratory tests showed its applicability to practical use. (author)

Analysis of simultaneous measurement of temperature and strain using different combinations of FBG

Science.gov (United States)

Ashik T., J.; Kachare, Nitin; Kalyani bai, K.; Kumar, D. Sriram

2017-06-01

The Fiber Bragg Grating (FBG) can be used for measuring temperature and or strain. In this paper analysis of different combinations of FBG is made. Certain parameters of FBG are considered such as Bandwidth, Side lobes, Peak power, and Sensitivity. Simultaneous measurement of temperature and strain is made using two combinations of FBG. The setup is simulated using two software. Optigrating 4.2.2 is used for designing different types of gratings such as Uniform, Apodized, Tilted and Superstructure. After designing, these files are exported to Optisystem 12 to simulate the spectrum and to observe the parameters.

Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures

DEFF Research Database (Denmark)

Olesen, Christian Gammelgaard; Pennisi, Cristian Pablo; de Zee, Mark

2013-01-01

tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis. Method The Flexcell system was redesigned using a finite element model in order to obtain large...... areas of the membrane in a controlled, uniform non-equibiaxial strain state. Results The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain....... Conclusion The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions....

Distributed Strain Measurement along a Concrete Beam via Stimulated Brillouin Scattering in Optical Fibers

Directory of Open Access Journals (Sweden)

Romeo Bernini

2011-01-01

Full Text Available The structural strain measurement of tension and compression in a 4 m long concrete beam was demonstrated with a distributed fiber-optic sensor portable system based on Brillouin scattering. Strain measurements provided by the fiber-optic sensor permitted to detect the formation of a crack in the beam resulting from the external applied load. The sensor system is valuable for structural monitoring applications, enabling the long-term performance and health of structures to be efficiently monitored.

Strain ratio measurement of femoral cartilage by real-time elastosonography: preliminary results

International Nuclear Information System (INIS)

Ipek, Ali; Unal, Ozlem; Kartal, Merve Gulbiz; Arslan, Halil; Isik, Cetin; Bozkurt, Murat

2015-01-01

The purpose of this study was to evaluate strain ratio measurement of femoral cartilage using real-time elastosonography. Twenty-five patients with femoral cartilage pathology on MRI (study group) were prospectively compared with 25 subjects with normal findings on MRI (control group) using real-time elastosonography. Strain ratio measurements of pathologic and normal cartilage were performed and compared, both within the study group and between the two groups. Elastosonography colour-scale coding showed a colour change from blue to red in pathologic cartilage and only blue colour-coding in normal cartilage. In the study group, the median strain ratio was higher in pathologic cartilage areas compared to normal areas (median, 1.49 [interquartile range, 0.80-2.53] vs. median, 0.01 [interquartile range, 0.01-0.01], p < 0.001, respectively). The median strain ratio of the control group was 0.01 (interquartile range, 0.01-0.01), and there was no significant difference compared to normal areas of the study group. There was, however, a significant difference between the control group cartilage and pathologic cartilage of the study group (p < 0.001). Elastosonography may be an effective, easily accessible, and relatively simple tool to demonstrate pathologic cartilage and to differentiate it from normal cartilage in the absence of advanced imaging facility such as MRI. (orig.)

A strain gauge

DEFF Research Database (Denmark)

2016-01-01

The invention relates to a strain gauge of a carrier layer and a meandering measurement grid positioned on the carrier layer, wherein the strain gauge comprises two reinforcement members positioned on the carrier layer at opposite ends of the measurement grid in the axial direction....... The reinforcement members are each placed within a certain axial distance to the measurement grid with the axial distance being equal to or smaller than a factor times the grid spacing. The invention further relates to a multi-axial strain gauge such as a bi-axial strain gauge or a strain gauge rosette where each...... of the strain gauges comprises reinforcement members. The invention further relates to a method for manufacturing a strain gauge as mentioned above....

Mapping of Mechanical Strains and Stresses around Quiescent Engineered Three-Dimensional Epithelial Tissues

Science.gov (United States)

Gjorevski, Nikolce; Nelson, Celeste M.

2012-01-01

Understanding how physical signals guide biological processes requires qualitative and quantitative knowledge of the mechanical forces generated and sensed by cells in a physiologically realistic three-dimensional (3D) context. Here, we used computational modeling and engineered epithelial tissues of precise geometry to define the experimental parameters that are required to measure directly the mechanical stress profile of 3D tissues embedded within native type I collagen. We found that to calculate the stresses accurately in these settings, we had to account for mechanical heterogeneities within the matrix, which we visualized and quantified using confocal reflectance and atomic force microscopy. Using this technique, we were able to obtain traction forces at the epithelium-matrix interface, and to resolve and quantify patterns of mechanical stress throughout the surrounding matrix. We discovered that whereas single cells generate tension by contracting and pulling on the matrix, the contraction of multicellular tissues can also push against the matrix, causing emergent compression. Furthermore, tissue geometry defines the spatial distribution of mechanical stress across the epithelium, which communicates mechanically over distances spanning hundreds of micrometers. Spatially resolved mechanical maps can provide insight into the types and magnitudes of physical parameters that are sensed and interpreted by multicellular tissues during normal and pathological processes. PMID:22828342

Measuring Local Strain Rates In Ductile Shear Zones: A New Approach From Deformed Syntectonic Dykes

Science.gov (United States)

Sassier, C.; Leloup, P.; Rubatto, D.; Galland, O.; Yue, Y.; Ding, L.

2006-12-01

At the Earth surface, deformation is mostly localized in fault zones in between tectonic plates. In the upper crust, the deformation is brittle and the faults are narrow and produce earthquakes. In contrast, deformation in the lower ductile crust results in larger shear zones. While it is relatively easy to measure in situ deformation rates at the surface using for example GPS data, it is more difficult to determinate in situ values of strain rate in the ductile crust. Such strain rates can only be estimated in paleo-shear zones. Various methods have been used to assess paleo-strain rates in paleo-shear zones. For instance, cooling and/or decompression rates associated with assumptions on geothermic gradients and shear zone geometry can lead to such estimates. Another way to estimate strain rates is the integration of paleo-stress measurements in a power flow law. But these methods are indirect and imply strong assumptions. Dating of helicitic garnets or syntectonic fibres are more direct estimates. However these last techniques have been only applied in zones of low deformation and not in major shear zones. We propose a new direct method to measure local strain rates in major ductile shear zones from syntectonic dykes by coupling quantification of deformation and geochronology. We test our method in a major shear zone in a well constrained tectonic setting: the Ailao-Shan - Red River Shear Zone (ASRRsz) located in SE Asia. For this 10 km wide shear zone, large-scale fault rates, determined in three independent ways, imply strain rates between 1.17×10^{-13 s-1 and 1.52×10^{-13 s-1 between 35 and 16 Ma. Our study focused on one outcrop where different generations of syntectonic dykes are observed. First, we quantified the minimum shear strain γ for each dyke using several methods: (1) by measuring the stretching of dykes with a surface restoration method (2) by measuring the final angle of the dykes with respect to the shear direction and (3) by combining the two

Spectral-Domain Measurement of Strain Sensitivity of a Two-Mode Birefringent Side-Hole Fiber

Directory of Open Access Journals (Sweden)

Waclaw Urbanczyk

2012-09-01

Full Text Available The strain sensitivity of a two-mode birefringent side-hole fiber is measured in the spectral domain. In a simple experimental setup comprising a broadband source, a polarizer, a two-mode birefringent side-hole fiber under varied elongations, an analyzer and a compact spectrometer, the spectral interferograms are resolved. These are characterized by the equalization wavelength at which spectral interference fringes have the highest visibility (the largest period due to the zero group optical path difference between the fundamental, the LP01 mode and the higher-order, the LP11 mode. The spectral interferograms with the equalization wavelength are processed to retrieve the phase as a function of the wavelength. From the retrieved phase functions corresponding to different elongations of a two-mode birefringent side-hole fiber under test, the spectral strain sensitivity is obtained. Using this approach, the intermodal spectral strain sensitivity was measured for both x and y polarizations. Moreover, the spectral polarimetric sensitivity to strain was measured for the fundamental mode when a birefringent delay line was used in tandem with the fiber. Its spectral dependence was also compared with that obtained from a shift of the spectral interferograms not including the equalization wavelength, and good agreement was confirmed.

Distributed strain measurement using modal interference in a birefringent optical fiber

International Nuclear Information System (INIS)

Kumar, Dilip; Sengupta, S; Ghorai, S K

2008-01-01

We propose a method based on modal interference in a birefringent optical fiber for distributed strain measurement. A frequency-modulated optical signal from a laser diode was launched in a birefringent fiber whose 'V' number was set in the range 2.405–3.831. The beat signal produced due to the interference of two lower order modes LP 01 and LP 11 was detected in a photodetector. The change in propagation constants between two modes under loading conditions leads to a phase variation of interference signal. The amplitude of the beat frequency was measured in the frequency domain by varying the modulating frequency, and it provides the measurand distribution along the beam. Results for strain distribution of a simply supported beam (51.0 cm × 3.0 cm × 0.6 cm) for an applied load of 250–1500 g are presented

Combining fibre optic Raman spectroscopy and tactile resonance measurement for tissue characterization

International Nuclear Information System (INIS)

Candefjord, Stefan; Nyberg, Morgan; Ramser, Kerstin; Lindahl, Olof A; Jalkanen, Ville

2010-01-01

Tissue characterization is fundamental for identification of pathological conditions. Raman spectroscopy (RS) and tactile resonance measurement (TRM) are two promising techniques that measure biochemical content and stiffness, respectively. They have potential to complement the golden standard-–histological analysis. By combining RS and TRM, complementary information about tissue content can be obtained and specific drawbacks can be avoided. The aim of this study was to develop a multivariate approach to compare RS and TRM information. The approach was evaluated on measurements at the same points on porcine abdominal tissue. The measurement points were divided into five groups by multivariate analysis of the RS data. A regression analysis was performed and receiver operating characteristic (ROC) curves were used to compare the RS and TRM data. TRM identified one group efficiently (area under ROC curve 0.99). The RS data showed that the proportion of saturated fat was high in this group. The regression analysis showed that stiffness was mainly determined by the amount of fat and its composition. We concluded that RS provided additional, important information for tissue identification that was not provided by TRM alone. The results are promising for development of a method combining RS and TRM for intraoperative tissue characterization

Noninvasive Doppler tissue measurement of pulmonary artery compliance in children with pulmonary hypertension.

Science.gov (United States)

Dyer, Karrie; Lanning, Craig; Das, Bibhuti; Lee, Po-Feng; Ivy, D Dunbar; Valdes-Cruz, Lilliam; Shandas, Robin

2006-04-01

We have shown previously that input impedance of the pulmonary vasculature provides a comprehensive characterization of right ventricular afterload by including compliance. However, impedance-based compliance assessment requires invasive measurements. Here, we develop and validate a noninvasive method to measure pulmonary artery (PA) compliance using ultrasound color M-mode (CMM) Doppler tissue imaging (DTI). Dynamic compliance (C(dyn)) of the PA was obtained from CMM DTI and continuous wave Doppler measurement of the tricuspid regurgitant velocity. C(dyn) was calculated as: [(D(s) - D(d))/(D(d) x P(s))] x 10(4); where D(s) = systolic diameter, D(d) = diastolic diameter, and P(s) = systolic pressure. The method was validated both in vitro and in 13 patients in the catheterization laboratory, and then tested on 27 pediatric patients with pulmonary hypertension, with comparison with 10 age-matched control subjects. C(dyn) was also measured in an additional 13 patients undergoing reactivity studies. Instantaneous diameter measured using CMM DTI agreed well with intravascular ultrasound measurements in the in vitro models. Clinically, C(dyn) calculated by CMM DTI agreed with C(dyn) calculated using invasive techniques (23.4 +/- 16.8 vs 29.1 +/- 20.6%/100 mm Hg; P = not significant). Patients with pulmonary hypertension had significantly lower peak wall velocity values and lower C(dyn) values than control subjects (P < .01). C(dyn) values followed an exponentially decaying relationship with PA pressure, indicating the nonlinear stress-strain behavior of these arteries. Reactivity in C(dyn) agreed with reactivity measured using impedance techniques. The C(dyn) method provides a noninvasive means of assessing PA compliance and should be useful as an additional measure of vascular reactivity subsequent to pulmonary vascular resistance in patients with pulmonary hypertension.

Clinical usefulness of facial soft tissues thickness measurement using 3D computed tomographic images

International Nuclear Information System (INIS)

Jeong, Ho Gul; Kim, Kee Deog; Hu, Kyung Seok; Lee, Jae Bum; Park, Hyok; Han, Seung Ho; Choi, Seong Ho; Kim, Chong Kwan; Park, Chang Seo

2006-01-01

To evaluate clinical usefulness of facial soft tissue thickness measurement using 3D computed tomographic images. One cadaver that had sound facial soft tissues was chosen for the study. The cadaver was scanned with a Helical CT under following scanning protocols about slice thickness and table speed: 3 mm and 3 mm/sec, 5 mm and 5 mm/sec, 7 mm and 7 mm/sec. The acquired data were reconstructed 1.5, 2.5, 3.5 mm reconstruction interval respectively and the images were transferred to a personal computer. Using a program developed to measure facial soft tissue thickness in 3D image, the facial soft tissue thickness was measured. After the ten-time repeation of the measurement for ten times, repeated measure analysis of variance (ANOVA) was adopted to compare and analyze the measurements using the three scanning protocols. Comparison according to the areas was analysed by Mann-Whitney test. There were no statistically significant intraobserver differences in the measurements of the facial soft tissue thickness using the three scanning protocols (p>0.05). There were no statistically significant differences between measurements in the 3 mm slice thickness and those in the 5 mm, 7 mm slice thickness (p>0.05). There were statistical differences in the 14 of the total 30 measured points in the 5 mm slice thickness and 22 in the 7 mm slice thickness. The facial soft tissue thickness measurement using 3D images of 7 mm slice thickness is acceptable clinically, but those of 5 mm slice thickness is recommended for the more accurate measurement

High-resolution analysis of the mechanical behavior of tissue

Science.gov (United States)

Hudnut, Alexa W.; Armani, Andrea M.

2017-06-01

The mechanical behavior and properties of biomaterials, such as tissue, have been directly and indirectly connected to numerous malignant physiological states. For example, an increase in the Young's Modulus of tissue can be indicative of cancer. Due to the heterogeneity of biomaterials, it is extremely important to perform these measurements using whole or unprocessed tissue because the tissue matrix contains important information about the intercellular interactions and the structure. Thus, developing high-resolution approaches that can accurately measure the elasticity of unprocessed tissue samples is of great interest. Unfortunately, conventional elastography methods such as atomic force microscopy, compression testing, and ultrasound elastography either require sample processing or have poor resolution. In the present work, we demonstrate the characterization of unprocessed salmon muscle using an optical polarimetric elastography system. We compare the results of compression testing within different samples of salmon skeletal muscle with different numbers of collagen membranes to characterize differences in heterogeneity. Using the intrinsic collagen membranes as markers, we determine the resolution of the system when testing biomaterials. The device reproducibly measures the stiffness of the tissues at variable strains. By analyzing the amount of energy lost by the sample during compression, collagen membranes that are 500 μm in size are detected.

Self-Evaluation of PANDA-FBG Based Sensing System for Dynamic Distributed Strain and Temperature Measurement.

Science.gov (United States)

Zhu, Mengshi; Murayama, Hideaki; Wada, Daichi

2017-10-12

A novel method is introduced in this work for effectively evaluating the performance of the PANDA type polarization-maintaining fiber Bragg grating (PANDA-FBG) distributed dynamic strain and temperature sensing system. Conventionally, the errors during the measurement are unknown or evaluated by using other sensors such as strain gauge and thermocouples. This will make the sensing system complicated and decrease the efficiency since more than one kind of sensor is applied for the same measurand. In this study, we used the approximately constant ratio of primary errors in strain and temperature measurement and realized the self-evaluation of the sensing system, which can significantly enhance the applicability, as well as the reliability in strategy making.

Measurement of {alpha} particle energy loss in biological tissue below 2 MeV

Energy Technology Data Exchange (ETDEWEB)

Stella, S. [Department of Nuclear and Theoretical Physics, University of Pavia, Pavia (Italy); National Institute of Nuclear Physics (INFN), Pavia (Italy); Bortolussi, S. [Department of Nuclear and Theoretical Physics, University of Pavia, Pavia (Italy); National Institute of Nuclear Physics (INFN), Pavia (Italy)], E-mail: silva.bortolussi@pv.infn.it; Bruschi, P.; Portella, C. [Department of Nuclear and Theoretical Physics, University of Pavia, Pavia (Italy); Altieri, S. [Department of Nuclear and Theoretical Physics, University of Pavia, Pavia (Italy); National Institute of Nuclear Physics (INFN), Pavia (Italy)

2009-09-01

The energy loss of {alpha} particles crossing biological tissue at energies between 0.8 and 2.2 MeV has been measured. This energy range is very important for boron neutron capture therapy, based on the {sup 10}B(n,{alpha}){sup 7}Li reaction, which emits {alpha} particles with energies of 1.78 and 1.47 MeV. One of the methods used for the measurement of the boron concentration in tissue is based on the deconvolution of the {alpha} spectra obtained from neutron irradiation of thin (70 {mu}m) tissue samples. For this technique, a knowledge of the behaviour of the energy loss of the particles in the irradiated tissue is of critical importance. In particular, the curve of the residual energy as a function of the distance travelled in the tissue must be known. In this paper, the results of an experiment carried out with an {sup 241}Am source and a series of cryostatic sections of rat-lung tissue are presented. The experimental measurements are compared with the results of Monte Carlo calculations performed with the MCNPX code.

Strain measurements of the tibial insert of a knee prosthesis using a knee motion simulator.

Science.gov (United States)

Sera, Toshihiro; Iwai, Yuya; Yamazaki, Takaharu; Tomita, Tetsuya; Yoshikawa, Hideki; Naito, Hisahi; Matsumoto, Takeshi; Tanaka, Masao

2017-12-01

The longevity of a knee prosthesis is influenced by the wear of the tibial insert due to its posture and movement. In this study, we assumed that the strain on the tibial insert is one of the main reasons for its wear and investigated the influence of the knee varus-valgus angles on the mechanical stress of the tibial insert. Knee prosthesis motion was simulated using a knee motion simulator based on a parallel-link six degrees-of-freedom actuator and the principal strain and pressure distribution of the tibial insert were measured. In particular, the early stance phase obtained from in vivo X-ray images was examined because the knee is applied to the largest load during extension/flexion movement. The knee varus-valgus angles were 0° (neutral alignment), 3°, and 5° malalignment. Under a neutral orientation, the pressure was higher at the middle and posterior condyles. The first and second principal strains were larger at the high and low pressure areas, respectively. Even for a 3° malalignment, the load was concentrated at one condyle and the positive first principal strain increased dramatically at the high pressure area. The negative second principal strain was large at the low pressure area on the other condyle. The maximum equivalent strain was 1.3-2.1 times larger at the high pressure area. For a 5° malalignment, the maximum equivalent strain increased slightly. These strain and pressure measurements can provide the mechanical stress of the tibial insert in detail for determining the longevity of an artificial knee joint.

Probing multi-scale mechanical damage in connective tissues using X-ray diffraction.

Science.gov (United States)

Bianchi, Fabio; Hofmann, Felix; Smith, Andrew J; Thompson, Mark S

2016-11-01

The accumulation of microstructural collagen damage following repetitive loading is linked to painful and debilitating tendon injuries. As a hierarchical, semi-crystalline material, collagen mechanics can be studied using X-ray diffraction. The aim of the study was to describe multi-structural changes in tendon collagen following controlled plastic damage (5% permanent strain). We used small angle X-ray scattering (SAXS) to interrogate the spacing of collagen molecules within a fibril, and wide angle X-ray scattering (WAXS) to measure molecular strains under macroscopic loading. Simultaneous recordings of SAXS and WAXS patterns, together with whole-tissue strain in physiologically hydrated rat-tail tendons were made during increments of in situ tensile loading. Results showed that while tissue level modulus was unchanged, fibril modulus decreased significantly, and molecular modulus significantly increased. Further, analysis of higher order SAXS peaks suggested structural changes in the gap and overlap regions, possibly localising the damage to molecular cross-links. Our results provide new insight into the fundamental damage processes at work in collagenous tissues and point to new directions for their mitigation and repair. This article reports the first in situ loading synchrotron studies on mechanical damage in collagenous tissues. We provide new insight into the nano- and micro-structural mechanisms of damage processes. Pre-damaged tendons showed differential alteration of moduli at macro, micro and nano-scales as measured using X-ray scattering techniques. Detailed analysis of higher order diffraction peaks suggested damage is localised to molecular cross-links. The results are consistent with previous X-ray scattering studies of tendons and also with recent thermal stability studies on damaged material. Detailed understanding of damage mechanisms is essential in the development of new therapies promoting tissue repair. Copyright © 2016 Acta Materialia Inc

Neutron Diffraction Residual Strain Tensor Measurements Within The Phase IA Weld Mock-up Plate P-5

Energy Technology Data Exchange (ETDEWEB)

Hubbard, Camden R [ORNL

2011-09-01

Oak Ridge National Laboratory (ORNL) has worked with NRC and EPRI to apply neutron and X-ray diffraction methods to characterize the residual stresses in a number of dissimilar metal weld mockups and samples. The design of the Phase IA specimens aimed to enable stress measurements by several methods and computational modeling of the weld residual stresses. The partial groove in the 304L stainless steel plate was filled with weld beads of Alloy 82. A summary of the weld conditions for each plate is provided in Table 1. The plates were constrained along the long edges during and after welding by bolts with spring-loaded washers attached to the 1-inch thick Al backing plate. The purpose was to avoid stress relief due to bending of the welded stainless steel plate. The neutron diffraction method was one of the methods selected by EPRI for non-destructive through thickness strain and stress measurement. Four different plates (P-3 to P-6) were studied by neutron diffraction strain mapping, representing four different welding conditions. Through thickness neutron diffraction strain mappings at NRSF2 for the four plates and associated strain-free d-zero specimens involved measurement along seven lines across the weld and at six to seven depths. The mountings of each plate for neutron diffraction measurements were such that the diffraction vector was parallel to each of the three primary orthogonal directions of the plate: two in-plane directions, longitudinal and transverse, and the direction normal to the plate (shown in left figure within Table 1). From the three orthogonal strains for each location, the residual stresses along the three plate directions were calculated. The principal axes of the strain and stress tensors, however, need not necessarily align with the plate coordinate system. To explore this, plate P-5 was selected for examination of the possibility that the principal axes of strain are not along the sample coordinate system axes. If adequate data could

Magneto-acousto-electrical Measurement Based Electrical Conductivity Reconstruction for Tissues.

Science.gov (United States)

Zhou, Yan; Ma, Qingyu; Guo, Gepu; Tu, Juan; Zhang, Dong

2018-05-01

Based on the interaction of ultrasonic excitation and magnetoelectrical induction, magneto-acousto-electrical (MAE) technology was demonstrated to have the capability of differentiating conductivity variations along the acoustic transmission. By applying the characteristics of the MAE voltage, a simplified algorithm of MAE measurement based conductivity reconstruction was developed. With the analyses of acoustic vibration, ultrasound propagation, Hall effect, and magnetoelectrical induction, theoretical and experimental studies of MAE measurement and conductivity reconstruction were performed. The formula of MAE voltage was derived and simplified for the transducer with strong directivity. MAE voltage was simulated for a three-layer gel phantom and the conductivity distribution was reconstructed using the modified Wiener inverse filter and Hilbert transform, which was also verified by experimental measurements. The experimental results are basically consistent with the simulations, and demonstrate that the wave packets of MAE voltage are generated at tissue interfaces with the amplitudes and vibration polarities representing the values and directions of conductivity variations. With the proposed algorithm, the amplitude and polarity of conductivity gradient can be restored and the conductivity distribution can also be reconstructed accurately. The favorable results demonstrate the feasibility of accurate conductivity reconstruction with improved spatial resolution using MAE measurement for tissues with conductivity variations, especially suitable for nondispersive tissues with abrupt conductivity changes. This study demonstrates that the MAE measurement based conductivity reconstruction algorithm can be applied as a new strategy for nondestructive real-time monitoring of conductivity variations in biomedical engineering.

New strain measurement method at axial tensile test of thin films through direct imaging

Energy Technology Data Exchange (ETDEWEB)

Ha, Jong-Eun [Department of Automotive Engineering, Seoul National Uinversity of Technolgy, 172 Gongneung-2 Dong, Nowon-Gu, Seoul (Korea, Republic of); Park, Jun-Hyub [Department of Mechatronics Engineering, College of Engineering, Tongmyong University, 535, Yongdang-Dong, Nam-Gu, Busan 608-711 (Korea, Republic of); Kang, Dong-Joong [School of Mechanical Eng., Pusan National University, Jangjeon-dong, Geumjeong-gu, Busan 609-735 (Korea, Republic of)], E-mail: jhyubpark@korea.com

2008-09-07

This paper proposes a new method for measuring strain during a tensile test of the specimen with micrometre size through direct imaging. A specimen was newly designed for adoption of direct imaging which was the main contribution of the proposed system. The structure of the specimen has eight indicators that make it possible to adopt direct imaging and it is fabricated using the same process of microelectromechanical system (MEMS) devices to guarantee the feasibility of the tensile test. We implemented a system for non-contact in situ measurement of strain with the specimen, the image-based displacement measurement system. Extension of the gauge length in the specimen could be found robustly by computing the positions of the eight rectangular-shape indicators on the image. Also, for an easy setup procedure, the region of interest was found automatically through the analysis of the edge projection profile along the horizontal direction. To gain confidence in the reliability of the system, the tensile test for the Al-3%Ti thin film was performed, which is widely used as a material in MEMS devices. Tensile tests were performed and displacements were measured using the proposed method and also the capacitance type displacement sensor for comparison. It is demonstrated that the new strain measurement system can be effectively used in the tensile test of the specimen at microscale with easy setup and better accuracy.

Measurement of histamine release from human lung tissue ex vivo by microdialysis technique

DEFF Research Database (Denmark)

Nissen, Dan; Petersen, Lars Jelstrup; Nolte, H

1998-01-01

OBJECTIVE AND DESIGN: Currently no method is available for measurement of mediator release from intact human lung. In this study, a microdialysis technique was used to measure histamine release from mast cells in human lung tissue ex vivo. MATERIAL: Microdialysis fibers of 216 microm were inserted...... responses were observed but data could be reproduced within individual donors. Monocyte chemoattractant protein-1, a potent basophil secretagogue, did not induce histamine release in lung tissue which indicated mast cells to be the histamine source. Substance P did not release histamine in the lung tissue....... CONCLUSIONS: The microdialysis technique allowed measurements of histamine release from mast cells in intact lung ex vivo. The method may prove useful since a number of experiments can be performed in a few hours in intact lung tissue without any dispersion or enzymatic treatment....

Molecular cloning of a Bangladeshi strain of very virulent infectious bursal disease virus of chickens and its adaptation in tissue culture by site-directed mutagenesis

International Nuclear Information System (INIS)

Islam, M.R.; Raue, R.; Mueller, H.

2005-01-01

Full-length cDNA of both genome segments of a Bangladeshi strain of very virulent infectious bursal disease virus (BD 3/99) were cloned in plasmid vectors along with the T7 promoter tagged to the 5'-ends. Mutations were introduced in the cloned cDNA to bring about two amino acid exchanges (Q253H and A284T) in the capsid protein VP2. Transfection of primary chicken embryo fibroblast cells with RNA transcribed in vitro from the full-length cDNA resulted in the formation of mutant infectious virus particles that grow in tissue culture. The pathogenicity of this molecularly-cloned, tissue-culture- adapted virus (BD-3tc) was tested in commercial chickens. The parental wild-type strain, BD 3/99, was included for comparison. The subclinical course of the disease and delayed bursal atrophy in BD-3tc-inoculated birds suggested that these amino acid substitutions made BD-3tc partially attenuated. (author)

Strain measurements of temperatures up to 3000C in a concrete structure

International Nuclear Information System (INIS)

Schittenhelm, Ch.

1975-10-01

Strain measurements in a concrete structure representing a cylindrical section of a Prestressed Concrete Reactor Vessel with hot liner, were made. In order to interpret these values in terms of strain and stress in a three dimensional structure, the gauge characteristics as well as the material date of the concrete have to be known in great detail. The paper deals with the performance of different gauges embedded in small concrete blocks and others of the same type embedded in the test ring. Temperature resistance, long-time performance, electrical drift and hysteresis of the gauges were investigated. (author)

Tissue tropisms, infection kinetics, histologic lesions, and antibody response of the MR766 strain of Zika virus in a murine model.

Science.gov (United States)

Kawiecki, Anna B; Mayton, E Handly; Dutuze, M Fausta; Goupil, Brad A; Langohr, Ingeborg M; Del Piero, Fabio; Christofferson, Rebecca C

2017-04-18

The appearance of severe Zika virus (ZIKV) disease in the most recent outbreak has prompted researchers to respond through the development of tools to quickly characterize transmission and pathology. We describe here another such tool, a mouse model of ZIKV infection and pathogenesis using the MR766 strain of virus that adds to the growing body of knowledge regarding ZIKV kinetics in small animal models. We infected mice with the MR766 strain of ZIKV to determine infection kinetics via serum viremia. We further evaluated infection-induced lesions via histopathology and visualized viral antigen via immunohistochemical labeling. We also investigated the antibody response of recovered animals to both the MR766 and a strain from the current outbreak (PRVABC59). We demonstrate that the IRF3/7 DKO mouse is a susceptible, mostly non-lethal model well suited for the study of infection kinetics, pathological progression, and antibody response. Infected mice presented lesions in tissues that have been associated with ZIKV infection in the human population, such as the eyes, male gonads, and central nervous system. In addition, we demonstrate that infection with the MR766 strain produces cross-neutralizing antibodies to the PRVABC59 strain of the Asian lineage. This model provides an additional tool for future studies into the transmission routes of ZIKV, as well as for the development of antivirals and other therapeutics, and should be included in the growing list of available tools for investigations of ZIKV infection and pathogenesis.

Residual strain dependence on the matrix structure in RHQ-Nb3Al wires by neutron diffraction measurement

International Nuclear Information System (INIS)

Jin Xinzhe; Nakamoto, Tatsushi; Tsuchiya, Kiyosumi; Ogitsu, Toru; Yamamoto, Akira; Ito, Takayoshi; Harjo, Stefanus; Kikuchi, Akihiro; Takeuchi, Takao; Hemmi, Tsutomu

2012-01-01

We prepared three types of non-Cu RHQ-Nb 3 Al wire sample with different matrix structures: an all-Ta matrix, a composite matrix of Nb and Ta with a Ta inter-filament, and an all-Nb matrix. Neutron diffraction patterns of the wire samples were measured at room temperature in the J-PARC ‘TAKUMI’. To obtain the residual strains of the materials, we estimated the lattice constant a by multi-peak analysis in the wires. A powder sample of each wire was measured, where the powder was considered to be strain free. The grain size of all the powder samples was below 0.02 mm. For the wire sample with the all-Nb matrix, we also obtained the lattice spacing d by a single-peak analysis. The residual strains of the Nb 3 Al filament were estimated from the two analysis results and were compared. The resulting residual strains obtained from the multi-peak analysis showed a good accuracy with small standard deviation. The multi-peak analysis results for the residual strains of the Nb 3 Al filaments in the three samples (without Cu plating) were all tensile residual strain in the axial direction, of 0.12%, 0.12%, and 0.05% for the all-Ta matrix, the composite matrix, and the all-Nb matrix, respectively. The difference in the residual strain of the Nb 3 Al filament between the composite and all-Nb matrix samples indicates that the type of inter-filament material shows a great effect on the residual strain. In this paper, we report the method of measurement, method of analysis, and results for the residual strain in the three types of non-Cu RHQ-Nb 3 Al wires. (paper)

A fiber optic strain measurement and quench localization for use in superconducting accelerator dipole magnets

International Nuclear Information System (INIS)

van Oort, J.M.; Scanlan, R.M.; ten Kate, H.H.J.

1994-01-01

A novel fiber-optic measurement system for superconducting accelerator magnets is described. The principal component is an extrinsic Fabry-Perot Interferometer to determine localized strain and stress in coil windings. The system can be used either as a sensitive relative strain measurement system or as an absolute strain detector. Combined, one can monitor the mechanical behaviour of the magnet system over time during construction, long time storage and operation. The sensing mechanism is described, together with various tests in laboratory environments. The test results of a multichannel test matrix to be incorporated first in the dummy coils and then in the final version of a 13T Nb 3 Sn accelerator dipole magnet are presented. Finally, the possible use of this system as a quench localization system is proposed

Tissue equivalent detector measurements on Mir space station. Comparison with other data

Energy Technology Data Exchange (ETDEWEB)

Bottollier-Depois, J.F. [CEA Centre d`Etudes de Fontenay-aux-Roses, 92 (France). Dept. de Protection de la Sante de l`Homme et de Dosimetrie; Siegrist, M. [Centre National d`Etudes Spatiales (CNES), 31 - Toulouse (France); Duvivier, E.; Almarcha, B. [STEEL Technologies, Mazeres sur Salat (France); Dachev, T.P.; Semkova, J.V. [Bulgarian Academy of Sciences, Sofia (Bulgaria). Central Lab. of Solar Energy and New Energy Sources; Petrov, V.M.; Bengin, V.; Koslova, S.B. [Institute of Biomedical Problems, Moscow (Russian Federation)

1995-12-31

The measurement of the dose received by the cosmonauts, due to cosmic radiations, during a space mission is an important parameter to estimate the radiological risk. Tissue equivalent measurements of radiation environment inside the MIR space station were performed continuously since July 1992. Interesting results about radiation measurements show (a) the South Atlantic Anomaly (SAA) crossing, (c) the increase of radiation near the poles and (d) the effects of solar eruptions. These data are compared with solid state detector (SSD) and other tissue equivalent proportional counter (TEPC) results. (authors). 4 refs., 7 figs.

Tissue equivalent detector measurements on Mir space station. Comparison with other data

International Nuclear Information System (INIS)

Bottollier-Depois, J.F.; Duvivier, E.; Almarcha, B.; Dachev, T.P.; Semkova, J.V.

1995-01-01

The measurement of the dose received by the cosmonauts, due to cosmic radiations, during a space mission is an important parameter to estimate the radiological risk. Tissue equivalent measurements of radiation environment inside the MIR space station were performed continuously since July 1992. Interesting results about radiation measurements show (a) the South Atlantic Anomaly (SAA) crossing, (c) the increase of radiation near the poles and (d) the effects of solar eruptions. These data are compared with solid state detector (SSD) and other tissue equivalent proportional counter (TEPC) results. (authors). 4 refs., 7 figs

Thermal Expansion and Magnetostriction Measurements at Cryogenic Temperature Using the Strain Gauge Method.

Science.gov (United States)

Wang, Wei; Liu, Huiming; Huang, Rongjin; Zhao, Yuqiang; Huang, Chuangjun; Guo, Shibin; Shan, Yi; Li, Laifeng

2018-01-01

Thermal expansion and magnetostriction, the strain responses of a material to temperature and a magnetic field, especially properties at low temperature, are extremely useful to study electronic and phononic properties, phase transitions, quantum criticality, and other interesting phenomena in cryogenic engineering and materials science. However, traditional dilatometers cannot provide magnetic field and ultra-low temperature (thermal expansion and magnetostriction at cryogenic temperature using the strain gauge method based on a Physical Properties Measurements System (PPMS). The interfacing software and automation were developed using LabVIEW. The sample temperature range can be tuned continuously between 1.8 and 400 K. With this PPMS-aided measuring system, we can observe temperature and magnetic field dependence of the linear thermal expansion of different solid materials easily and accurately.

Non-invasive tissue temperature measurements based on quantitative diffuse optical spectroscopy (DOS) of water

Energy Technology Data Exchange (ETDEWEB)

Chung, S H [Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, PA 19104 (United States); Cerussi, A E; Tromberg, B J [Beckman Laser Institute and Medical Clinic, University of California, Irvine, 1002 Health Sciences Road, Irvine 92612, CA (United States); Merritt, S I [Masimo Corporation, 40 Parker, Irvine, CA 92618 (United States); Ruth, J, E-mail: bjtrombe@uci.ed [Department of Bioengineering, University of Pennsylvania, 210 S. 33rd Street, Room 240, Skirkanich Hall, Philadelphia, PA 19104 (United States)

2010-07-07

We describe the development of a non-invasive method for quantitative tissue temperature measurements using Broadband diffuse optical spectroscopy (DOS). Our approach is based on well-characterized opposing shifts in near-infrared (NIR) water absorption spectra that appear with temperature and macromolecular binding state. Unlike conventional reflectance methods, DOS is used to generate scattering-corrected tissue water absorption spectra. This allows us to separate the macromolecular bound water contribution from the thermally induced spectral shift using the temperature isosbestic point at 996 nm. The method was validated in intralipid tissue phantoms by correlating DOS with thermistor measurements (R = 0.96) with a difference of 1.1 {+-} 0.91 {sup 0}C over a range of 28-48 {sup 0}C. Once validated, thermal and hemodynamic (i.e. oxy- and deoxy-hemoglobin concentration) changes were measured simultaneously and continuously in human subjects (forearm) during mild cold stress. DOS-measured arm temperatures were consistent with previously reported invasive deep tissue temperature studies. These results suggest that DOS can be used for non-invasive, co-registered measurements of absolute temperature and hemoglobin parameters in thick tissues, a potentially important approach for optimizing thermal diagnostics and therapeutics.

Spherical Nanoindentation Stress-Strain Measurements of BOR-60 14YWT-NFA1 Irradiated Tubes

Energy Technology Data Exchange (ETDEWEB)

Weaver, Jordan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Carvajal Nunez, Ursula [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Krumwiede, David [Univ. of California, Berkeley, CA (United States); Saleh, Tarik A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Hosemann, Peter [Univ. of California, Berkeley, CA (United States); Nelson, Andrew Thomas [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Maloy, Stuart Andrew [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Mara, Nathan Allan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-09-28

Spherical nanoindentation stress-strain protocols were applied to characterize unirradiated and fast neutron irradiated nanostructured ferritic alloy (NFA) 14YWT and compared against Berkovich nanohardness and available tensile data. The predicted uniaxial yield strength from spherical, 100 and 5 micron radii, indentation yield strength measurements was 1100-1400 MPa which compares well with the predictions from Berkovich nanohardness, 1200 MPa, and available tensile data, ~1100 MPa. However, spherical indentation measurements predict an increase in the uniaxial yield strength of ~1 GPa while Berkovich nanohardness measurements predict an increase of only ~250 MPa. No tensile data exists on the irradiated condition. It is believed the difference in the predicted uniaxial yield strength between spherical and Berkovich nanoindentation are due to a low number of tests on the irradiated sample combined with the significant heterogeneity in the microstructure, the differences in sensitivity to sample preparation on the irradiated sample between the two indentation protocols , and/or in how strain localizes under the indenter with the possibility of dislocation channeling under Berkovich hardness indents leading to strain softening. Nanoindentation capabilities to test neutron irradiated samples in a radiological area were realized.

Comparisons of Soft Tissue Thickness Measurements in Adult Patients With Various Vertical Patterns

Directory of Open Access Journals (Sweden)

Neslihan Seyhan Cezairli

2017-08-01

Full Text Available Objective: The purposes of this study were to evaluate to study soft tissue facial profile among the different vertical patterns using the Holdaway analysis and the soft tissue thickness measurements. Materials and Methods: The study sample consisted of 90 patients divided into 3 groups: low angle group (30 patients; mean age, 20.38±3.76 years, normal angle group (30 patients; mean age, 19.36±2.83 years and high angle group (30 patients; mean age, 19.44±2.14 years. The study sample, comprised a total of 90 patients (54 women and 36 men divided into low-angle, normal-angle and high angle groups based on vertical growth pattern using the SN/GoGn angle (high-angle group >37°; low-angle group <27°; and control group or normal angle group 27-37°. Facial soft-tissue thickness and Holdaway measurements were analyzed on each radiograph with Image J programme. One-way analysis of variance and post-hoc test (Tukey were used to compare Holdaway measurements and soft tissue thicknesses among the three groups. Results: Significant differences among vertical patterns were observed for the ‘gnathion’, ‘menton’, ‘stomion’ and ‘inferior sulcus to H line’ when both genders were combined. These measurements were thinner in the high-angle group. Significant differences among vertical patterns were observed for ‘gnathion’ and ‘lower lip to H line’ in women; for ‘stomion’ and ‘nose prominence’ in men when examined separately. Conclusion: Facial soft tissue measurements except some for in high angle group were thinner than in low angle group. All soft tissue measurements were greater except for gnathion in low angle group in men than in women.

Fiber Strain Measurement for Wide Region Quasidistributed Sensing by Optical Correlation Sensor with Region Separation Techniques

Directory of Open Access Journals (Sweden)

Xunjian Xu

2010-01-01

Full Text Available The useful application of optical pulse correlation sensor for wide region quasidistributed fiber strain measurement is investigated. Using region separation techniques of wavelength multiplexing with FBGs and time multiplexing with intensity partial reflectors, the sensor measures the correlations between reference pulses and monitoring pulses from several cascadable selected sensing regions. This novel sensing system can select the regions and obtain the distributed strain information in any desired sensing region.

Tissue expression of Toll-like receptors 2, 3, 4 and 7 in swine in response to the Shimen strain of classical swine fever virus

Science.gov (United States)

Cao, Zhi; Zheng, Minping; Lv, Huifang; Guo, Kangkang; Zhang, Yanming

2018-01-01

The Toll-like receptors (TLRs) of the innate immune system provide the host with the ability to detect and respond to viral infections. The present study aimed to investigate the mRNA and protein expression levels of TLR2, 3, 4 and 7 in porcine tissues upon infection with the highly virulent Shimen strain of classical swine fever virus (CSFV). Reverse transcription-quantitative polymerase chain reaction was used to detect the mRNA expression levels of CSFV and TLR, whereas western blotting was used to detect the expression levels of TLR proteins. In addition, tissues underwent histological examination and immunohistochemistry to reveal the histopathological alterations associated with highly virulent CSFV infection and to detect TLR antigens. Furthermore, porcine monocyte-derived macrophages (pMDMs) were prestimulated with peptidoglycan from Staphylococcus aureus (PGN-SA), polyinosinic-polycytidylic acid [poly (I:C)], lipopolysaccharide from Escherichia coli 055:B5 (LPS-B5) or imiquimod (R837) in order to analyze the association between TLR expression and CSFV replication. Following stimulation for 12 h (with TLR-specific ligands), cells were infected with CSFV Shimen strain. The results revealed that the expression levels of TLR2 and TLR4 were increased in the lung and kidney, but were decreased in the spleen and lymph nodes in response to CSFV. TLR3 was strongly expressed in the heart and slightly upregulated in the spleen in response to CSFV Shimen strain infection, and TLR7 was increased in all examined tissues in the presence of CSFV. Furthermore, R837 and LPS-B5 exerted inhibitory effects on CSFV replication in pMDMs, whereas PGN-SA and poly(I:C) had no significant effect. These findings highlight the potential role of TLR expression in the context of CSFV infection. PMID:29568891

Multiwavelength Raman-fiber-laser-based long-distance remote sensor for simultaneous measurement of strain and temperature.

Science.gov (United States)

Han, Young-Geun; Tran, T V A; Kim, Sang-Hyuck; Lee, Sang Bae

2005-06-01

We propose a simple and flexible multiwavelength Raman-fiber-laser-based long-distance remote-sensing scheme for simultaneous measurement of strain and temperature by use of fiber Bragg gratings. By combining two uniform fiber Bragg gratings with a tunable chirped fiber grating, we readily achieve simultaneous two-channel sensing probes with a high extinction ratio of more than approximately 50 dB over a 50-km distance. When strain and temperature are applied, lasing wavelength separation and shift occur, respectively, since the two uniform fiber Bragg gratings have identical material composition and different cladding diameters. This allows simultaneous measurement of strain and temperature for long-distance sensing applications of more than 50 km.

Ultrasound elastography assessment of bone/soft tissue interface

International Nuclear Information System (INIS)

Parmar, Biren J; Yang, Xu; Chaudhry, Anuj; Shajudeen, Peer Shafeeq; Nair, Sanjay P; Righetti, Raffaella; Weiner, Bradley K; Tasciotti, Ennio; Krouskop, Thomas A

2016-01-01

We report on the use of elastographic imaging techniques to assess the bone/soft tissue interface, a region that has not been previously investigated but may provide important information about fracture and bone healing. The performance of axial strain elastograms and axial shear strain elastograms at the bone/soft tissue interface was studied ex vivo on intact and fractured canine and ovine tibias. Selected ex vivo results were corroborated on intact sheep tibias in vivo. The elastography results were statistically analyzed using elastographic image quality tools. The results of this study demonstrate distinct patterns in the distribution of the normalized local axial strains and axial shear strains at the bone/soft tissue interface with respect to the background soft tissue. They also show that the relative strength and distribution of the elastographic parameters change in the presence of a fracture and depend on the degree of misalignment between the fracture fragments. Thus, elastographic imaging modalities might be used in the future to obtain information regarding the integrity of bones and to assess the severity of fractures, alignment of bone fragments as well as to follow bone healing. (paper)

Ultrasound elastography assessment of bone/soft tissue interface

Science.gov (United States)

Parmar, Biren J.; Yang, Xu; Chaudhry, Anuj; Shafeeq Shajudeen, Peer; Nair, Sanjay P.; Weiner, Bradley K.; Tasciotti, Ennio; Krouskop, Thomas A.; Righetti, Raffaella

2016-01-01

We report on the use of elastographic imaging techniques to assess the bone/soft tissue interface, a region that has not been previously investigated but may provide important information about fracture and bone healing. The performance of axial strain elastograms and axial shear strain elastograms at the bone/soft tissue interface was studied ex vivo on intact and fractured canine and ovine tibias. Selected ex vivo results were corroborated on intact sheep tibias in vivo. The elastography results were statistically analyzed using elastographic image quality tools. The results of this study demonstrate distinct patterns in the distribution of the normalized local axial strains and axial shear strains at the bone/soft tissue interface with respect to the background soft tissue. They also show that the relative strength and distribution of the elastographic parameters change in the presence of a fracture and depend on the degree of misalignment between the fracture fragments. Thus, elastographic imaging modalities might be used in the future to obtain information regarding the integrity of bones and to assess the severity of fractures, alignment of bone fragments as well as to follow bone healing.

More plant availability by local and integral strain measurement

Energy Technology Data Exchange (ETDEWEB)

Hulshof, H J.M.; Welberg, P G.M. [KEMA Nederland B.V., Arnhem (Netherlands); Bruijn, L.E. de [E.ON Benelux Generation N.V., Rotterdam (Netherlands). Power Plant Maasvlakte

2002-07-01

Industrial installations that are under pressure and are operating at high temperatures have a limited life due to creep and fatigue. It is, therefore, of critical importance to know the location of any possible weak spots in the installation. Welds in steam pipes, especially the heat-affected zones in these welds, are such weak spots in the long term. The material deforms and cracks may develop, with significant failure in the worst case. To avoid safety risks, unforeseen plant shutdown and, as a consequence, high costs for unavailability and repair, periodic inspections and strain measurements are recommended. KEMA's SPICA (Speckle Image Correlation Analysis) system is able to measure on-stream (during operation) deformation due to creep in critical areas, like the heat-affected zone in welds. (orig.)

Combined full field stress and strain measurement methods for granular materials

Directory of Open Access Journals (Sweden)

Broere W.

2010-06-01

Full Text Available The current paper re-introduces the photoelastic measurement method in experimental geomechanics. A full-field phase stepping polariscope suitable for geomechanical model tests has been developed. Additional constraints on the measurement and mechanical setup arising from geomechanical test conditions are outlined as well as the opportunity to measure the displacement fields in the sample with digital image correlation. The capability of the current setup in retrieving the stress and strain field in a granular material is demonstrated.

Measurement of the hyperelastic properties of 44 pathological ex vivo breast tissue samples

International Nuclear Information System (INIS)

O'Hagan, Joseph J; Samani, Abbas

2009-01-01

The elastic and hyperelastic properties of biological soft tissues have been of interest to the medical community. There are several biomedical applications where parameters characterizing such properties are critical for a reliable clinical outcome. These applications include surgery planning, needle biopsy and brachtherapy where tissue biomechanical modeling is involved. Another important application is interpreting nonlinear elastography images. While there has been considerable research on the measurement of the linear elastic modulus of small tissue samples, little research has been conducted for measuring parameters that characterize the nonlinear elasticity of tissues included in tissue slice specimens. This work presents hyperelastic measurement results of 44 pathological ex vivo breast tissue samples. For each sample, five hyperelastic models have been used, including the Yeoh, N = 2 polynomial, N = 1 Ogden, Arruda-Boyce, and Veronda-Westmann models. Results show that the Yeoh, polynomial and Ogden models are the most accurate in terms of fitting experimental data. The results indicate that almost all of the parameters corresponding to the pathological tissues are between two times to over two orders of magnitude larger than those of normal tissues, with C 11 showing the most significant difference. Furthermore, statistical analysis indicates that C 02 of the Yeoh model, and C 11 and C 20 of the polynomial model have very good potential for cancer classification as they show statistically significant differences for various cancer types, especially for invasive lobular carcinoma. In addition to the potential for use in cancer classification, the presented data are very important for applications such as surgery planning and virtual reality based clinician training systems where accurate nonlinear tissue response modeling is required.

Measurement of the hyperelastic properties of 44 pathological ex vivo breast tissue samples

Energy Technology Data Exchange (ETDEWEB)

O' Hagan, Joseph J; Samani, Abbas [Department of Electrical and Computer Engineering, University of Western Ontario, London, ON (Canada)], E-mail: asamani@uwo.ca

2009-04-21

The elastic and hyperelastic properties of biological soft tissues have been of interest to the medical community. There are several biomedical applications where parameters characterizing such properties are critical for a reliable clinical outcome. These applications include surgery planning, needle biopsy and brachtherapy where tissue biomechanical modeling is involved. Another important application is interpreting nonlinear elastography images. While there has been considerable research on the measurement of the linear elastic modulus of small tissue samples, little research has been conducted for measuring parameters that characterize the nonlinear elasticity of tissues included in tissue slice specimens. This work presents hyperelastic measurement results of 44 pathological ex vivo breast tissue samples. For each sample, five hyperelastic models have been used, including the Yeoh, N = 2 polynomial, N = 1 Ogden, Arruda-Boyce, and Veronda-Westmann models. Results show that the Yeoh, polynomial and Ogden models are the most accurate in terms of fitting experimental data. The results indicate that almost all of the parameters corresponding to the pathological tissues are between two times to over two orders of magnitude larger than those of normal tissues, with C{sub 11} showing the most significant difference. Furthermore, statistical analysis indicates that C{sub 02} of the Yeoh model, and C{sub 11} and C{sub 20} of the polynomial model have very good potential for cancer classification as they show statistically significant differences for various cancer types, especially for invasive lobular carcinoma. In addition to the potential for use in cancer classification, the presented data are very important for applications such as surgery planning and virtual reality based clinician training systems where accurate nonlinear tissue response modeling is required.

Measurement of the hyperelastic properties of 44 pathological ex vivo breast tissue samples

Science.gov (United States)

O'Hagan, Joseph J.; Samani, Abbas

2009-04-01

The elastic and hyperelastic properties of biological soft tissues have been of interest to the medical community. There are several biomedical applications where parameters characterizing such properties are critical for a reliable clinical outcome. These applications include surgery planning, needle biopsy and brachtherapy where tissue biomechanical modeling is involved. Another important application is interpreting nonlinear elastography images. While there has been considerable research on the measurement of the linear elastic modulus of small tissue samples, little research has been conducted for measuring parameters that characterize the nonlinear elasticity of tissues included in tissue slice specimens. This work presents hyperelastic measurement results of 44 pathological ex vivo breast tissue samples. For each sample, five hyperelastic models have been used, including the Yeoh, N = 2 polynomial, N = 1 Ogden, Arruda-Boyce, and Veronda-Westmann models. Results show that the Yeoh, polynomial and Ogden models are the most accurate in terms of fitting experimental data. The results indicate that almost all of the parameters corresponding to the pathological tissues are between two times to over two orders of magnitude larger than those of normal tissues, with C11 showing the most significant difference. Furthermore, statistical analysis indicates that C02 of the Yeoh model, and C11 and C20 of the polynomial model have very good potential for cancer classification as they show statistically significant differences for various cancer types, especially for invasive lobular carcinoma. In addition to the potential for use in cancer classification, the presented data are very important for applications such as surgery planning and virtual reality based clinician training systems where accurate nonlinear tissue response modeling is required.

Effect-independent measures of tissue responses to fractionated irradiation

International Nuclear Information System (INIS)

Thames, H.D. Jr.

1984-01-01

Tissue repair factors measure the sparing that can be achieved from dose fractionation in the absence of proliferation. Four repair factors are analysed in these terms: Fsub(R),Fsub(rec), the ratio of linear-quadratic survival model parameters β/α and the half-time Tsub(1/2) for intracellular repair processes. Theoretically, Fsub(R) and Fsub(rec) are increasing functions of D 1 , and thus depend on level of effect. This is confirmed by analysis of skin reactions after multifractionated radiation. By contrast, β/α is effect-independent as a measure of repair capacity in skin, gut, and bone marrow, tissues for which it is reasonable to assume that survival of identifiable target cells is the primary determinant of the endpoint. For a functional endpoint not clearly connected with the depletion of a specific target-cell population (late fibrotic reactions in the kidney), there was an increase in β/α with increased levels of injury, but this was statistically insignificant. Tsub(1/2) is independent of fraction size in skin, gut, and spinal cord, and is longer (1.5 hours) in the late-reacting tissues (lung and spinal cord) than in those that react acutely (Tsub(1/2) less than 1 hour), with skin as the exception (Tsub(1/2) approx. 1.3 hours). (author)

In-Vivo Techniques for Measuring Electrical Properties of Tissues.

Science.gov (United States)

1980-09-01

probe Electromagnetic energy Dielectric properties Monopole antenna In-situ tissues , Antemortem/Pos tmortem studies Renal blood flow 10 ABSTRACT... mice or rats, which were positioned beneath a fixed measurement probe. Several alternative methods involving the use of semi-rigid or flexible coaxial

Synchrotron measurements of local microstructure and residual strains in ductile cast iron

DEFF Research Database (Denmark)

Zhang, Yubin; Andriollo, Tito; Fæster, Søren

2017-01-01

The local microstructure and distribution of thermally induced residual strains in ferrite matrix grains around an individual spherical graphite nodule in ductile cast iron (DCI) were measured using a synchrotron X-ray micro-diffraction technique. It is found that the matrix grains are deformed...

Evaluation of Pressure Pain Threshold as a Measure of Perceived Stress and High Job Strain

DEFF Research Database (Denmark)

Hven, Lisbeth; Frost, Poul; Bonde, Jens Peter Ellekilde

2017-01-01

OBJECTIVE: To investigate whether pressure pain threshold (PPT), determined by pressure algometry, can be used as an objective measure of perceived stress and job strain. METHODS: We used cross-sectional base line data collected during 1994 to 1995 within the Project on Research and Intervention...... in Monotonous work (PRIM), which included 3123 employees from a variety of Danish companies. Questionnaire data included 18 items on stress symptoms, 23 items from the Karasek scale on job strain, and information on discomfort in specified anatomical regions was also collected. Clinical examinations included...... pressure pain algometry measurements of PPT on the trapezius and supraspinatus muscles and the tibia. Associations of stress symptoms and job strain with PPT of each site was analyzed for men and women separately with adjustment for age body mass index, and discomfort in the anatomical region closest...

Performance Characteristics and Prediction of Bodyweight using Linear Body Measurements in Four Strains of Broiler Chicken

OpenAIRE

I. Udeh; J.O. Isikwenu and G. Ukughere

2011-01-01

The objectives of this study were to compare the performance characteristics of four strains of broiler chicken from 2 to 8 weeks of age and predict body weight of the broilers using linear body measurements. The four strains of broiler chicken used were Anak, Arbor Acre, Ross and Marshall. The parameters recorded were bodyweight, weight gain, total feed intake, feed conversion ratio, mortality and some linear body measurements (body length, body width, breast width, drumstick length, shank l...

Rehabilitation effect of exercise with soft tissue manipulation in ...

African Journals Online (AJOL)

Rehabilitation effect of exercise with soft tissue manipulation in patients with lumbar muscle strain. H Li, H Zhang, S Liu, Y Wang, D Gai, Q Lu, H Gan, Y Shi, W Qi. Abstract. Objective: To study the rehabilitation effect of exercise with soft tissue manipulation therapy for patients with lumbar muscle strain. Methods: Patients with ...

X-ray measurement of plastic strain by means of Eshelby/Mori-Tanaka model and its application

International Nuclear Information System (INIS)

Sasaki, Toshihiko; Lin, Zheng; Hirose, Yukio

1997-01-01

A new method is proposed in this paper for determining plastic strains in composite materials using the X-ray diffraction method. The present method was derived by using both Eshelby's approach and the Mori-Tanaka theory to express the stress state in composite materials instead of the elasticity in single-phase materials which is used in the conventional method of X-ray stress measurement. It was found that the plastic strain can be determined from the slope of the linear relation between lattice strains measured by the X-ray diffraction technique and sin 2 ψ using almost the same procedure as that for determining stresses by the conventional X-ray method. The results on ferritic and austenitic dual-phase stainless steel are shown. We discuss the effects of a uniaxial tensile load in a range of plastic deformation on the field of plastic strain as well as on residual macro-, micro- and phase stresses built up in the sample. (author)

Geometry of X-ray based measurement of residual strain at desired penetration depth

Energy Technology Data Exchange (ETDEWEB)

Morawiec, A. [Polish Academy of Sciences, Institute of Metallurgy and Materials Science, Krakow (Poland)

2017-10-15

X-ray based measurement of residual lattice strains at chosen penetration depth is one of the methods for investigating strain inhomogeneities in near-surface layers of polycrystalline materials. The measurement relies on determining shifts of Bragg peaks for various directions of the scattering vector with respect to the specimen. At each of these directions, to reach a given the penetration depth, a proper specimen orientation is required. The task of determining such orientations, albeit elementary, is quite intricate. The existing literature describes only partial solutions with unspecified domains of application, which fail if applied to beyond the domains. Therefore, geometric aspects of the measurement are analyzed in details. Explicit bounds on measurement parameters are given. The equation fundamental for the procedure is solved with respect to specimen orientations. For a given direction of the scattering vector, there are generally four different specimen orientations leading to the same penetration depth. This simple fact (overlooked in previous analyses) can be used for improving reliability of measurement results. Analytical formulas for goniometer angles representing these orientations are provided. (copyright 2017 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Progress of a Cross-Correlation Based Optical Strain Measurement Technique for Detecting Radial Growth on a Rotating Disk

Science.gov (United States)

Clem, Michelle M.; Abdul-Aziz, Ali; Woike, Mark R.; Fralick, Gustave C.

2015-01-01

The modern turbine engine operates in a harsh environment at high speeds and is repeatedly exposed to combined high mechanical and thermal loads. The cumulative effects of these external forces lead to high stresses and strains on the engine components, such as the rotating turbine disks, which may eventually lead to a catastrophic failure if left undetected. The operating environment makes it difficult to use conventional strain gauges, therefore, non-contact strain measurement techniques is of interest to NASA and the turbine engine community. This presentation describes one such approach; the use of cross correlation analysis to measure strain experienced by the engine turbine disk with the goal of assessing potential faults and damage.

Thermal expansion measurement of turbine and main steam piping by using strain gages in power plants

International Nuclear Information System (INIS)

Na, Sang Soo; Chung, Jae Won; Bong, Suk Kun; Jun, Dong Ki; Kim, Yun Suk

2000-01-01

One of the domestic co-generation plants have undergone excessive vibration problems of turbine attributed to external force for years. The root cause of turbine vibration may be shaft alignment problem which sometimes is changed by thermal expansion and external force, even if turbine technicians perfectly performed it. To evaluate the alignment condition from plant start-up to full load, a strain measurement of turbine and main steam piping subjected to thermal loading is monitored by using strain gages. The strain gages are bonded on both bearing housing adjusting bolts and pipe stoppers which installed in the x-direction of left-side main steam piping near the turbine inlet in order to monitor closely the effect of turbine under thermal deformation of turbine casing and main steam piping during plant full load. Also in situ load of constant support hangers in main steam piping system is measured by strain gages and its results are used to rebalance the hanger rod load. Consequently, the experimental stress analysis by using strain gages turns out to be very useful tool to diagnose the trouble and failures of not only to stationary components but to rotating machinery in power plants

A principle for the noninvasive measurement of steady-state heat transfer parameters in living tissues

Directory of Open Access Journals (Sweden)

S. Yu. Makarov

2014-01-01

Full Text Available Measuring the parameters of biological tissues (include in vivo is of great importance for medical diagnostics. For example, the value of the blood perfusion parameter is associated with the state of the blood microcirculation system and its functioning affects the state of the tissues of almost all organs. This work describes a previously proposed principle [1] in generalized terms. The principle is intended for noninvasive measuring the parameters of stationary heat transfer in biological tissues. The results of some experiments (natural and numeric are also presented in the research.For noninvasive measurement of thermophysical parameters a number of techniques have been developed using non-stationary thermal process in biological tissue [2][3]. But these techniques require the collecting a lot of data to represent the time-dependent thermal signal. In addition, subsequent processing with specialized algorithms is required for optimal selecting the parameters. The goal of this research is to develop an alternative approach using stationary thermal process for non-invasive measuring the parameters of stationary heat transfer in living tissues.A general principle can be formulated for the measurement methods based on this approach. Namely, the variations (changes of two physical values are measured in the experiment at the transition from one thermal stationary state to another. One of these two physical values unambiguously determines the stationary thermal field into the biological tissue under specified experimental conditions while the other one is unambiguously determined through the thermal field. Then, the parameters can be found from the numerical (or analytical functional dependencies linking the measured variations because the dependencies contain unknown parameters.The dependencies are expressed in terms of the formula:dqi = fi({pj},Ui dUi,Here dqi is a variation of a physical value q which is unambiguously determined from the

A flow cytometry-based assay for quantifying non-plaque forming strains of yellow fever virus.

Directory of Open Access Journals (Sweden)

Erika Hammarlund

Full Text Available Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE on cell monolayers. In our hands, the Dakar strain of yellow fever virus (YFV-Dakar could not be measured by plaque assay (PA, focus-forming assay (FFA, or by measurement of CPE. For these reasons, we developed a YFV-specific monoclonal antibody (3A8.B6 and used it to optimize a highly sensitive flow cytometry-based tissue culture limiting dilution assay (TC-LDA to measure levels of infectious virus. The TC-LDA was performed by incubating serial dilutions of virus in replicate wells of C6/36 cells and stained intracellularly for virus with MAb 3A8.B6. Using this approach, we could reproducibly quantitate YFV-Dakar in tissue culture supernatants as well as from the serum of viremic rhesus macaques experimentally infected with YFV-Dakar. Moreover, the TC-LDA approach was >10-fold more sensitive than standard plaque assay for quantitating typical plaque-forming strains of YFV including YFV-17D and YFV-FNV (French neurotropic vaccine. Together, these results indicate that the TC-LDA technique is effective for quantitating both plaque-forming and non-plaque-forming strains of yellow fever virus, and this methodology may be readily adapted for the study and quantitation of other non-plaque-forming viruses.

Effective X-ray elastic constant measurement for in situ stress measurement of biaxially strained AA5754-O

International Nuclear Information System (INIS)

Iadicola, Mark A.; Gnäupel-Herold, Thomas H.

2012-01-01

Accurate measurement of stresses by X-ray diffraction requires accurate X-ray elastic constants. Calibration experiments are one method to determine these for a specific material in a specific condition. In this paper, uniaxial tension experiments are used to investigate the variation of these constants after uniaxial and equal-biaxial plastic deformation for an aluminum alloy (AA5754-O) of interest to the automotive industry. These data are critical for accurate measurement of the biaxial mechanical properties of the material using a recent experimental method combining specialized sheet metal forming equipment with portable X-ray diffraction equipment. The measured effective X-ray elastic constants show some minor variation with increased plastic deformation, and this behavior was found to be consistent for both uniaxially and equal-biaxially strained samples. The use of two average values for effective X-ray elastic constants, one in the rolling direction and one transverse to the rolling direction of the sheet material, is shown to be of sufficient accuracy for the combined tests of interest. Comparison of uniaxial data measured using X-ray diffraction and standard methods show good agreement, and biaxial stress–strain results show good repeatability. Additionally, the calibration data show some non-linear behavior, which is analyzed in regards to crystallographic texture and intergranular stress effects. The non-linear behavior is found to be the result of intergranular stresses based on comparison with additional measurements using other X-ray diffraction equipment and neutron diffraction.

Are there sex differences in Fetal Abdominal Subcutaneous Tissue (FAST) measurements?

LENUS (Irish Health Repository)

Farah, Nadine

2012-02-01

OBJECTIVE: To determine if Fetal Abdominal Subcutaneous Tissue (FAST) measurements using antenatal ultrasound differ between male and female fetuses. STUDY DESIGN: Women who had an ultrasound examination for fetal growth between 20 and 40 weeks gestation were studied. Women with diabetes mellitus were excluded. The fetal anterior abdominal subcutaneous tissue was measured on the anterior abdominal wall in millimetres anterior to the margins of the ribs, using magnification at the level of the abdominal circumference. The fetal sex was recorded after delivery. RESULTS: A total of 557 fetuses were measured, 290 male and 267 female. The FAST measurements increased with gestational age. The FAST increased at the same rate for both male and female fetuses and at any given week there was no sex difference. CONCLUSIONS: The increased fat composition in females reported after birth was not found in abdominal wall subcutaneous fat measurements using ultrasound during pregnancy. Antenatal centile charts for FAST do not need to be based on sex.

Simulation and Experimental Investigations on the Strain Measurement of the Uniform Strength Beam Using a FBG sensor

International Nuclear Information System (INIS)

Tu Yumeng; Gong Huaping; Chen Jixuan; Jin Yongxing

2011-01-01

The model of force analysis on a uniform strength beam is built by the general finite element program (ANSYS software). The flexivity profile produced by uniform strength beam with different forces is simulated by ANSYS software. In experiment, a fiber Bragg grating sensor is fixed on the uniform strength beam with modified acrylate. The flexivity and strain are varied by changing the load on the end of the beam. The strain of the uniform strength beam is measured with FBG when applied force is varied from 2.45N to14.7N with a step of 2.45N. Both the simulated and experimental results show that, the strain induced by the uniform strength beam is linear with the load force. The sensitivity is 18.32με/N for experimental measurement, and 19.72με/N for simulation. The experimental results are consistent with the simulation results, with the maximum measurement error of strain being 7.4%. It indicates that, the FBG sensor fixed with modified acrylate is proved to be effectively and reliably in the applications of civil engineering.

Cutting force measurement of electrical jigsaw by strain gauges

International Nuclear Information System (INIS)

Kazup, L; Varadine Szarka, A

2016-01-01

This paper describes a measuring method based on strain gauges for accurate specification of electric jigsaw's cutting force. The goal of the measurement is to provide an overall perspective about generated forces in a jigsaw's gearbox during a cutting period. The lifetime of the tool is affected by these forces primarily. This analysis is part of the research and development project aiming to develop a special linear magnetic brake for realizing automatic lifetime tests of electric jigsaws or similar handheld tools. The accurate specification of cutting force facilitates to define realistic test cycles during the automatic lifetime test. The accuracy and precision resulted by the well described cutting force characteristic and the possibility of automation provide new dimension for lifetime testing of the handheld tools with alternating movement. (paper)

Accuracy and reliability of facial soft tissue depth measurements using cone beam computer tomography

NARCIS (Netherlands)

Fourie, Zacharias; Damstra, Janalt; Gerrits, Pieter; Ren, Yijin

2010-01-01

It is important to have accurate and reliable measurements of soft tissue thickness for specific landmarks of the face and scalp when producing a facial reconstruction. In the past several methods have been created to measure facial soft tissue thickness (FSTT) in cadavers and in the living. The

Insulin action in adipose tissue and muscle in hypothyroidism.

Science.gov (United States)

Dimitriadis, George; Mitrou, Panayota; Lambadiari, Vaia; Boutati, Eleni; Maratou, Eirini; Panagiotakos, Demosthenes B; Koukkou, Efi; Tzanela, Marinela; Thalassinos, Nikos; Raptis, Sotirios A

2006-12-01

Although insulin resistance in thyroid hormone excess is well documented, information on insulin action in hypothyroidism is limited. To investigate this, a meal was given to 11 hypothyroid (HO; aged 45 +/- 3 yr) and 10 euthyroid subjects (EU; aged 42 +/- 4 yr). Blood was withdrawn for 360 min from veins (V) draining the anterior abdominal sc adipose tissue and the forearm and from the radial artery (A). Blood flow (BF) in adipose tissue was measured with 133Xe and in forearm with strain-gauge plethysmography. Tissue glucose uptake was calculated as (A-V)glucose(BF), lipoprotein lipase as (A-V)Triglycerides(BF), and lipolysis as [(V-A)glycerol(BF)]-lipoprotein lipase. The HO group had higher glucose and insulin levels than the EU group (P hypothyroidism: 1) glucose uptake in muscle and adipose tissue is resistant to insulin; 2) suppression of lipolysis by insulin is not impaired; and 3) hypertriglyceridemia is due to decreased clearance by the adipose tissue.

Investigation of the interpolation method to improve the distributed strain measurement accuracy in optical frequency domain reflectometry systems.

Science.gov (United States)

Cui, Jiwen; Zhao, Shiyuan; Yang, Di; Ding, Zhenyang

2018-02-20

We use a spectrum interpolation technique to improve the distributed strain measurement accuracy in a Rayleigh-scatter-based optical frequency domain reflectometry sensing system. We demonstrate that strain accuracy is not limited by the "uncertainty principle" that exists in the time-frequency analysis. Different interpolation methods are investigated and used to improve the accuracy of peak position of the cross-correlation and, therefore, improve the accuracy of the strain. Interpolation implemented by padding zeros on one side of the windowed data in the spatial domain, before the inverse fast Fourier transform, is found to have the best accuracy. Using this method, the strain accuracy and resolution are both improved without decreasing the spatial resolution. The strain of 3 μϵ within the spatial resolution of 1 cm at the position of 21.4 m is distinguished, and the measurement uncertainty is 3.3 μϵ.

Taxonomy of Streptomyces strains isolated from rhizospheres of ...

African Journals Online (AJOL)

Taxonomy of Streptomyces strains isolated from rhizospheres of various plant species grown in Taif region, KSA, having antagonistic activities against some microbial tissue ... African Journal of Biotechnology ... Keywords: Taxonomy, Streptomyces, microbial tissue culture contaminants, antagonistic activities, 16S rRNA

Response of a uniform optical fiber Bragg grating to strain with a non-smooth distribution: measurements and simulations

Science.gov (United States)

Detka, Małgorzata

2017-08-01

The paper presents results of numerical analyses of the response of a uniform fiber Bragg grating subjected to a strain with non-smooth profile. Results of measurements of the response of the grating to a compressive strain correspond well with results of the simulation and show, that the induced strain profile of the grating causes a widening of its reflection spectrum with a considerable shape irregularity, dependent on the location of the point where slope of the strain profile changes abruptly, and on the maximum value of the strain.

Development of nondestructive hybrid measuring method for three-dimensional residual stress distribution of thick welded joint. Hybrid measuring method of inherent strain method and neutron diffraction method

International Nuclear Information System (INIS)

Nakacho, Keiji; Kasahara, Norifumi; Tamura, Ryota

2012-01-01

The measuring methods of the residual stress are classified into destructive one and nondestructive one. The inherent strain method (ISM) is destructive one. The neutron diffraction method (NDM) is nondestructive one. But the measurable depth is limited within about 20 mm and the method cannot measure the weld zone, without destruction of the object. So, in this study, the hybrid measuring method has been developed, by combining the ISM and the NDM. The theory of the hybrid method is the same as the ISM. In the analysis, the strains measured by the NDM without destruction are used. This hybrid measuring method is a true nondestructive measuring method for a thick welded joint. The applicability of the hybrid method has been verified by simulation, using a butt welded joint of thick pipes. In the simulation, the reliable order of the strains measured by the present NDM is very important, and was considered as 10 micro. The measurable regions by the present NDM were assumed. Under the above conditions, the data (the residual elastic strains assumed to be measured by the NDM) were made, and used in the ISM. As a result of such simulation, it has been cleared that the estimated residual stress has very high accuracy, if enough data are used. The required number of data is less than the ISM. (author)

Thermal Expansion and Magnetostriction Measurements at Cryogenic Temperature Using the Strain Gauge Method

Directory of Open Access Journals (Sweden)

Wei Wang

2018-03-01

Full Text Available Thermal expansion and magnetostriction, the strain responses of a material to temperature and a magnetic field, especially properties at low temperature, are extremely useful to study electronic and phononic properties, phase transitions, quantum criticality, and other interesting phenomena in cryogenic engineering and materials science. However, traditional dilatometers cannot provide magnetic field and ultra-low temperature (<77 K environment easily. This paper describes the design and test results of thermal expansion and magnetostriction at cryogenic temperature using the strain gauge method based on a Physical Properties Measurements System (PPMS. The interfacing software and automation were developed using LabVIEW. The sample temperature range can be tuned continuously between 1.8 and 400 K. With this PPMS-aided measuring system, we can observe temperature and magnetic field dependence of the linear thermal expansion of different solid materials easily and accurately.

Thermal expansion and magnetostriction measurements at cryogenic temperature using the strain gage method

Science.gov (United States)

Wang, Wei; Liu, Huiming; Huang, Rongjin; Zhao, Yuqiang; Huang, Chuangjun; Guo, Shibin; Shan, Yi; Li, Laifeng

2018-03-01

Thermal expansion and magnetostriction, the strain responses of a material to temperature and a magnetic field, especially properties at low temperature, are extremely useful to study electronic and phononic properties, phase transitions, quantum criticality, and other interesting phenomena in cryogenic engineering and materials science. However, traditional dilatometers cannot provide magnetic field and ultra low temperature (＜77 K) environment easily. This paper describes the design and test results of thermal expansion and magnetostriction at cryogenic temperature using the strain gage method based on a Physical Properties Measurements System (PPMS). The interfacing software and automation were developed using LabVIEW. The sample temperature range can be tuned continuously between 1.8 K and 400 K. With this PPMS-aided measuring system, we can observe temperature and magnetic field dependence of the linear thermal expansion of different solid materials easily and accurately.

A Tissue Propagation Model for Validating Close-Proximity Biomedical Radiometer Measurements

Science.gov (United States)

Bonds, Q.; Herzig, P.; Weller, T.

2016-01-01

The propagation of thermally-generated electromagnetic emissions through stratified human tissue is studied herein using a non-coherent mathematical model. The model is developed to complement subsurface body temperature measurements performed using a close proximity microwave radiometer. The model takes into account losses and reflections as thermal emissions propagate through the body, before being emitted at the skin surface. The derivation is presented in four stages and applied to the human core phantom, a physical representation of a stomach volume of skin, muscle, and blood-fatty tissue. A drop in core body temperature is simulated via the human core phantom and the response of the propagation model is correlated to the radiometric measurement. The results are comparable, with differences on the order of 1.5 - 3%. Hence the plausibility of core body temperature extraction via close proximity radiometry is demonstrated, given that the electromagnetic characteristics of the stratified tissue layers are known.

Design and Validation of a Cyclic Strain Bioreactor to Condition Spatially-Selective Scaffolds in Dual Strain Regimes

Directory of Open Access Journals (Sweden)

J. Matthew Goodhart

2014-03-01

Full Text Available The objective of this study was to design and validate a unique bioreactor design for applying spatially selective, linear, cyclic strain to degradable and non-degradable polymeric fabric scaffolds. This system uses a novel three-clamp design to apply cyclic strain via a computer controlled linear actuator to a specified zone of a scaffold while isolating the remainder of the scaffold from strain. Image analysis of polyethylene terephthalate (PET woven scaffolds subjected to a 3% mechanical stretch demonstrated that the stretched portion of the scaffold experienced 2.97% ± 0.13% strain (mean ± standard deviation while the unstretched portion experienced 0.02% ± 0.18% strain. NIH-3T3 fibroblast cells were cultured on the PET scaffolds and half of each scaffold was stretched 5% at 0.5 Hz for one hour per day for 14 days in the bioreactor. Cells were checked for viability and proliferation at the end of the 14 day period and levels of glycosaminoglycan (GAG and collagen (hydroxyproline were measured as indicators of extracellular matrix production. Scaffolds in the bioreactor showed a seven-fold increase in cell number over scaffolds cultured statically in tissue culture plastic petri dishes (control. Bioreactor scaffolds showed a lower concentration of GAG deposition per cell as compared to the control scaffolds largely due to the great increase in cell number. A 75% increase in hydroxyproline concentration per cell was seen in the bioreactor stretched scaffolds as compared to the control scaffolds. Surprisingly, little differences were experienced between the stretched and unstretched portions of the scaffolds for this study. This was largely attributed to the conditioned and shared media effect. Results indicate that the bioreactor system is capable of applying spatially-selective, linear, cyclic strain to cells growing on polymeric fabric scaffolds and evaluating the cellular and matrix responses to the applied strains.

Lactococcus lactis Metabolism and Gene Expression during Growth on Plant Tissues

Science.gov (United States)

Golomb, Benjamin L.

2014-01-01

Lactic acid bacteria have been isolated from living, harvested, and fermented plant materials; however, the adaptations these bacteria possess for growth on plant tissues are largely unknown. In this study, we investigated plant habitat-specific traits of Lactococcus lactis during growth in an Arabidopsis thaliana leaf tissue lysate (ATL). L. lactis KF147, a strain originally isolated from plants, exhibited a higher growth rate and reached 7.9-fold-greater cell densities during growth in ATL than the dairy-associated strain L. lactis IL1403. Transcriptome profiling (RNA-seq) of KF147 identified 853 induced and 264 repressed genes during growth in ATL compared to that in GM17 laboratory culture medium. Genes induced in ATL included those involved in the arginine deiminase pathway and a total of 140 carbohydrate transport and metabolism genes, many of which are involved in xylose, arabinose, cellobiose, and hemicellulose metabolism. The induction of those genes corresponded with L. lactis KF147 nutrient consumption and production of metabolic end products in ATL as measured by gas chromatography-time of flight mass spectrometry (GC-TOF/MS) untargeted metabolomic profiling. To assess the importance of specific plant-inducible genes for L. lactis growth in ATL, xylose metabolism was targeted for gene knockout mutagenesis. Wild-type L. lactis strain KF147 but not an xylA deletion mutant was able to grow using xylose as the sole carbon source. However, both strains grew to similarly high levels in ATL, indicating redundancy in L. lactis carbohydrate metabolism on plant tissues. These findings show that certain strains of L. lactis are well adapted for growth on plants and possess specific traits relevant for plant-based food, fuel, and feed fermentations. PMID:25384484

[Biocybernetic approach to the thermometric methods of blood supply measurements of periodontal tissues].

Science.gov (United States)

Pastusiak, J; Zakrzewski, J

1988-11-01

Specific biocybernetic approach to the problem of the blood supply determination of paradontium tissues by means of thermometric methods has been presented in the paper. The compartment models of the measuring procedure have been given. Dilutodynamic methology and classification has been applied. Such an approach enables to select appropriate biophysical parameters describing the state of blood supply of paradontium tissues and optimal design of transducers and measuring methods.

Strain-encoding cardiovascular magnetic resonance for assessment of right-ventricular regional function

Directory of Open Access Journals (Sweden)

Abraham M Roselle

2008-07-01

Full Text Available Abstract Background Tissue tagging by cardiovascular magnetic resonance (CMR is a comprehensive method for the assessment of cardiac regional function. However, imaging the right ventricle (RV using this technique is problematic due to the thin wall of the RV relative to tag spacing which limits assessment of regional function using conventional in-plane tagging. Hypothesis We hypothesize that the use of through-plane tags in the strain-encoding (SENC CMR technique would result in reproducible measurements of the RV regional function due to the high image quality and spatial resolution possible with SENC. Aim To test the intra- and inter-observer variabilities of RV peak systolic strain measurements with SENC CMR for assessment of RV regional function (systolic strain in healthy volunteers. Methods Healthy volunteers (n = 21 were imaged using SENC. A four-chamber view was acquired in a single breath-hold. Circumferential strain was measured during systole at six equidistant points along the RV free wall. Peak contraction is defined as the maximum value of circumferential strain averaged from the six points, and regional function is defined as the strain value at each point at the time of peak contraction. Results Mean values for peak circumferential strain (± standard deviation of the basal, mid, and apical regions of the RV free wall were -20.4 ± 2.9%, -18.8 ± 3.9%, and -16.5 ± 5.7%, Altman plots showed good intra- and inter-observer agreements with mean difference of 0.11% and 0.32% and limits of agreement of -4.038 to 4.174 and -4.903 to 5.836, respectively. Conclusion SENC CMR allows for rapid quantification of RV regional function with low intra- and inter-observer variabilities, which could permit accurate quantification of regional strain in patients with RV dysfunction.

Design and implementation of an x-ray strain measurement capability using a rotating anode machine

Energy Technology Data Exchange (ETDEWEB)

Roberts, J.A.; Rangaswamy, P.; Lujan, M. Jr.; Bourke, M.A.M.

1998-12-31

This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Residual stresses close to the surface can improve the reliability and lifetime of parts for technological applications. X-ray diffraction plays a significant role in gaining an exact knowledge of the stresses at the surface and their depth distribution. An x-ray capability at Los Alamos is key to developing and maintaining industrial collaborations in strain effects. To achieve this goal, the authors implemented a residual strain measuring station on the rotating anode x-ray instrument at the Lujan Center. This capability has been used to investigate residual strains in heat treated automotive components, machining effects on titanium alloys, resistance welded steel joints, titanium matrix fiber reinforced composites, ceramic matrix composites, thin films, and ceramic coatings. The overall objective is to combine both x-ray and neutron diffraction measurements with numerical models (e.g., finite element calculations).

Strain Measurement of Steel Roof Truss Using FBG Sensor during Construction of Reverse Shell Shaped Reinforced Concrete Structure

Energy Technology Data Exchange (ETDEWEB)

Lee, Kun Woo [Kyungpook National University, Daegu (Korea, Republic of); Rhim, Hong Chul; Seo, Tae Seok [Yonsei University, Seoul (Korea, Republic of)

2011-08-15

Application of FBG (Fiber Bragg Grating) sensors to measure strain of steel roof trusses has been performed. This is to check and confirm the structural integrity of an unusually shaped, reverse shell structure made of reinforced concrete. The issue was to place sensors at proper location and compare the measured values to the results from structural analysis. It has been learned that a deliberate measurement scheme is needed in order to monitor a complex structure during construction. In this study, the measured values were within allowable range of strain, thus confirming the safety of the structure during measurement and construction.

[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].

Science.gov (United States)

Zhu, Bo; Liu, Jing-Jing; Si, Jin-Ping; Qin, Lu-Ping; Han, Ting; Zhao, Li; Wu, Ling-Shang

2016-05-01

The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings. Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured. Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively. Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively. It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus. Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts. The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings. The strains tested, expect DO12, could promote the accumulation of mannose. Furthermore, DO18, DO19 and DO120 could increase alcohol-soluble extracts. On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering. Copyright© by the Chinese Pharmaceutical Association.

Voxel-based measurement sensitivity of spatially resolved near-infrared spectroscopy in layered tissues.

Science.gov (United States)

Niwayama, Masatsugu

2018-03-01

We quantitatively investigated the measurement sensitivity of spatially resolved spectroscopy (SRS) across six tissue models: cerebral tissue, a small animal brain, the forehead of a fetus, an adult brain, forearm muscle, and thigh muscle. The optical path length in the voxel of the model was analyzed using Monte Carlo simulations. It was found that the measurement sensitivity can be represented as the product of the change in the absorption coefficient and the difference in optical path length in two states with different source-detector distances. The results clarified the sensitivity ratio between the surface layer and the deep layer at each source-detector distance for each model and identified changes in the deep measurement area when one of the detectors was close to the light source. A comparison was made with the results from continuous-wave spectroscopy. The study also identified measurement challenges that arise when the surface layer is inhomogeneous. Findings on the measurement sensitivity of SRS at each voxel and in each layer can support the correct interpretation of measured values when near-infrared oximetry or functional near-infrared spectroscopy is used to investigate different tissue structures. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

A simple indentation device for measuring micrometer-scale tissue stiffness

Energy Technology Data Exchange (ETDEWEB)

Levental, I; Levental, K R; Janmey, P A [Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104 (United States); Klein, E A; Assoian, R [Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104 (United States); Miller, R T [Departments of Medicine and Physiology, Louis Stokes VAMC, Cleveland, OH (United States); Wells, R G, E-mail: janmey@mail.med.upenn.ed [Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 (United States)

2010-05-19

Mechanical properties of cells and extracellular matrices are critical determinants of function in contexts including oncogenic transformation, neuronal synapse formation, hepatic fibrosis and stem cell differentiation. The size and heterogeneity of biological specimens and the importance of measuring their mechanical properties under conditions that resemble their environments in vivo present a challenge for quantitative measurement. Centimeter-scale tissue samples can be measured by commercial instruments, whereas properties at the subcellular (nm) scale are accessible by atomic force microscopy, optical trapping, or magnetic bead microrheometry; however many tissues are heterogeneous on a length scale between micrometers and millimeters which is not accessible to most current instrumentation. The device described here combines two commercially available technologies, a micronewton resolution force probe and a micromanipulator for probing soft biological samples at sub-millimeter spatial resolution. Several applications of the device are described. These include the first measurement of the stiffness of an intact, isolated mouse glomerulus, quantification of the inner wall stiffness of healthy and diseased mouse aortas, and evaluation of the lateral heterogeneity in the stiffness of mouse mammary glands and rat livers with correlation of this heterogeneity with malignant or fibrotic pathology as evaluated by histology.

High strain-rate soft material characterization via inertial cavitation

Science.gov (United States)

Estrada, Jonathan B.; Barajas, Carlos; Henann, David L.; Johnsen, Eric; Franck, Christian

2018-03-01

Mechanical characterization of soft materials at high strain-rates is challenging due to their high compliance, slow wave speeds, and non-linear viscoelasticity. Yet, knowledge of their material behavior is paramount across a spectrum of biological and engineering applications from minimizing tissue damage in ultrasound and laser surgeries to diagnosing and mitigating impact injuries. To address this significant experimental hurdle and the need to accurately measure the viscoelastic properties of soft materials at high strain-rates (103-108 s-1), we present a minimally invasive, local 3D microrheology technique based on inertial microcavitation. By combining high-speed time-lapse imaging with an appropriate theoretical cavitation framework, we demonstrate that this technique has the capability to accurately determine the general viscoelastic material properties of soft matter as compliant as a few kilopascals. Similar to commercial characterization algorithms, we provide the user with significant flexibility in evaluating several constitutive laws to determine the most appropriate physical model for the material under investigation. Given its straightforward implementation into most current microscopy setups, we anticipate that this technique can be easily adopted by anyone interested in characterizing soft material properties at high loading rates including hydrogels, tissues and various polymeric specimens.

Strain measurement of objects subjected to aerodynamic heating using digital image correlation: experimental design and preliminary results.

Science.gov (United States)

Pan, Bing; Jiang, Tianyun; Wu, Dafang

2014-11-01

In thermomechanical testing of hypersonic materials and structures, direct observation and quantitative strain measurement of the front surface of a test specimen directly exposed to severe aerodynamic heating has been considered as a very challenging task. In this work, a novel quartz infrared heating device with an observation window is designed to reproduce the transient thermal environment experienced by hypersonic vehicles. The specially designed experimental system allows the capture of test article's surface images at various temperatures using an optical system outfitted with a bandpass filter. The captured images are post-processed by digital image correlation to extract full-field thermal deformation. To verify the viability and accuracy of the established system, thermal strains of a chromiumnickel austenite stainless steel sample heated from room temperature up to 600 °C were determined. The preliminary results indicate that the air disturbance between the camera and the specimen due to heat haze induces apparent distortions in the recorded images and large errors in the measured strains, but the average values of the measured strains are accurate enough. Limitations and further improvements of the proposed technique are discussed.

Diffuse reflectance spectroscopy for the measurement of tissue oxygen saturation

International Nuclear Information System (INIS)

Sircan-Kucuksayan, A; Canpolat, M; Uyuklu, M

2015-01-01

Tissue oxygen saturation (StO 2 ) is a useful parameter for medical applications. A spectroscopic method has been developed to detect pathologic tissues, due to a lack of normal blood circulation, by measuring StO 2 . In this study, human blood samples with different levels of oxygen saturation have been prepared and spectra were acquired using an optical fiber probe to investigate the correlation between the oxygen saturation levels and the spectra. A linear correlation between the oxygen saturation and ratio of the intensities (760 nm to 790 nm) of the spectra acquired from blood samples has been found. In a validation study, oxygen saturations of the blood samples were estimated from the spectroscopic measurements with an error of 2.9%. It has also been shown that the linear dependence between the ratio and the oxygen saturation of the blood samples was valid for the blood samples with different hematocrits. Spectra were acquired from the forearms of 30 healthy volunteers to estimate StO 2 prior to, at the beginning of, after 2 min, and at the release of total vascular occlusion. The average StO 2 of a forearm before and after the two minutes occlusion was significantly different. The results suggested that optical reflectance spectroscopy is a sensitive method to estimate the StO 2 levels of human tissue. The technique developed to measure StO 2 has potential to detect ischemia in real time. (paper)

Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

Science.gov (United States)

Matthysse, A G; Wyman, P M; Holmes, K V

1978-11-01

Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five Ti-plasmid-containing virulent Agrobacterium strains showed greater attachment to tobacco cells than did five avirulent strains. Light and scanning electron microscopic observations confirmed that virulent strains showed little attachment. Bacterial attachment was blocked by prior incubation of the plant cells with lipopolysaccharide extracted from A. tumefaciens, but not from A. radiobacter, suggesting that bacterial lipopolysaccharide is one of the components involved in the attachment process. At least one other bacterial product may be required for attachment in tissue culture because the virulent A. tumefaciens NT1, which lacks the Ti plasmid, does not itself attach to tobacco cells, but its lipopolysaccharide does inhibit the attachment of virulent strains.

Measurement of minute local strain in semiconductor materials and electronic devices by using a highly parallel X-ray microbeam

International Nuclear Information System (INIS)

Matsui, J.; Tsusaka, Y.; Yokoyama, K.; Takeda, S.; Katou, M.; Kurihara, H.; Watanabe, K.; Kagoshima, Y.; Kimura, S.

2003-01-01

We have developed an X-ray microbeam with a small angular divergence by adopting X-ray optics with successive use of asymmetric Bragg reflection from silicon crystals for the both polarizations of the synchrotron X-rays. The microbeam actually obtained is several microns in size and possesses an angular divergence of less than 2 arcsec which enables us to measure the strain of 10 -5 -10 -6 . By scanning the sample against the microbeam, distribution of the minute local strain in various regions of semiconductor crystals for electronic devices, e.g., the strain around the SiO 2 /Si film edge in silicon devices, the strain in an InGaAsP/InP stripe laser were measured

Hemodynamic measurements in deep brain tissues of humans by near-infrared time-resolved spectroscopy

Science.gov (United States)

Suzuki, Hiroaki; Oda, Motoki; Yamaki, Etsuko; Suzuki, Toshihiko; Yamashita, Daisuke; Yoshimoto, Kenji; Homma, Shu; Yamashita, Yutaka

2014-03-01

Using near-infrared time-resolved spectroscopy (TRS), we measured the human head in transmittance mode to obtain the optical properties, tissue oxygenation, and hemodynamics of deep brain tissues in 50 healthy adult volunteers. The right ear canal was irradiated with 3-wavelengths of pulsed light (760, 795, and 835nm), and the photons passing through the human head were collected at the left ear canal. Optical signals with sufficient intensity could be obtained from 46 of the 50 volunteers. By analyzing the temporal profiles based on the photon diffusion theory, we successfully obtained absorption coefficients for each wavelength. The levels of oxygenated hemoglobin (HbO2), deoxygenated hemoglobin (Hb), total hemoglobin (tHb), and tissue oxygen saturation (SO2) were then determined by referring to the hemoglobin spectroscopic data. Compared with the SO2 values for the forehead measurements in reflectance mode, the SO2 values of the transmittance measurements of the human head were approximately 10% lower, and tHb values of the transmittance measurements were always lower than those of the forehead reflectance measurements. Moreover, the level of hemoglobin and the SO2 were strongly correlated between the human head measurements in transmittance mode and the forehead measurements in the reflectance mode, respectively. These results demonstrated a potential application of this TRS system in examining deep brain tissues of humans.

Measurement of lung tissue dynamics in artificially ventilated rats with optical coherence tomography

Directory of Open Access Journals (Sweden)

Schnabel Christian

2017-09-01

Full Text Available Diseases of lung tissue and the airways become a major task for medical care and health care systems in modern industrial countries in the future. Suitable treatment methods and strategies for lung support and artificial ventilation are of dare need. Besides the obvious importance as life-saving intervention, the effects of usually used over-pressure ventilation onto the sensitive alveolar tissue are insufficiently understood. Therefore, it is of great interest to characterize lung tissue during artificial ventilation at the alveolar level. Those measurements can be used to link micromechanics of alveolar structures to mechanical properties of the whole lung like compliance and resistance measured at the ventilator device. This can be done only in animal experiments due to the fact that imaging techniques used in human diagnostics like CT or MRT fail to resolve alveolar tissue structures. The disadvantage of high-resolution techniques like optical coherence tomography (OCT or intravital microscopy (IVM is the need of a surgical access to the lung due to the limitation in penetration depth of these techniques. Furthermore, imaging dynamic processes with high-resolution imaging techniques during uninterrupted artificial ventilation is a challenging task. In this study, we present a measurement setup for combined imaging of conventional pressure-controlled ventilated rats and the visualization of volume changes of alveolar structures during one cycle of breath. A custom-made OCT system in combination with a triggered scanning algorithm was used to acquire time-resolved 3D OCT image data. Furthermore, this system was combined with a self-adapting autofocus function for intravital microscopy to track the lung surface keeping the tissue in focal plane. The combination of new dynamic measurement modes for OCT and IVM allows new insights into alveolar tissue and will promote the understanding of mechanical behavior during artificial ventilation.

Evaluation of the dynamic behavior of a Pelton runner based on strain gauge measurements

Science.gov (United States)

Mack, Reiner; Probst, Christian

2016-11-01

A reliable mechanical design of Pelton runners is very important in the layout of new installations and modernizations. Especially in horizontal machines, where the housing is not embedded into concrete, a rupture of a runner bucket can have severe consequences. Even if a crack in the runner is detected on time, the outage time that follows the malfunction of the runner is shortening the return of investment. It is a fact that stresses caused by the runner rotation and the jet forces are superposed by high frequent dynamic stresses. In case of resonance it even can be the dominating effect that is limiting the lifetime of a runner. Therefore a clear understanding of the dynamic mechanisms is essential for a safe runner design. This paper describes the evaluation of the dynamic behavior of a Pelton runner installed in a model turbine based on strain gauge measurements. Equipped with strain gauges at the root area of the buckets, the time responses of the strains under the influence of various operational parameters were measured. As a result basic theories for the jet bucket excitation were verified and the influence of the water mass was detected by evaluating the frequency shift in case of resonance. Furthermore, the influence of the individual bucket masses onto the dynamic behaviour for different mode shapes got measured.

Prediction and measurement of thermally induced cambial tissue necrosis in tree stems

Science.gov (United States)

Joshua L. Jones; Brent W. Webb; Bret W. Butler; Matthew B. Dickinson; Daniel Jimenez; James Reardon; Anthony S. Bova

2006-01-01

A model for fire-induced heating in tree stems is linked to a recently reported model for tissue necrosis. The combined model produces cambial tissue necrosis predictions in a tree stem as a function of heating rate, heating time, tree species, and stem diameter. Model accuracy is evaluated by comparison with experimental measurements in two hardwood and two softwood...

Mechanical Characterization of Immature Porcine Brainstem in Tension at Dynamic Strain Rates.

Science.gov (United States)

Zhao, Hui; Yin, Zhiyong; Li, Kui; Liao, Zhikang; Xiang, Hongyi; Zhu, Feng

2016-01-21

Many brain injury cases involve pediatric road traffic accidents, and among these, brainstem injury causes disastrous outcomes. A thorough understanding of the tensile characterization of immature brainstem tissue is crucial in modeling traumatic brain injury sustained by children, but limited experimental data in tension is available for the immature brain tissue at dynamic strain rates. We harvested brainstem tissue from immature pigs (about 4 weeks old, and at a developmental stage similar to that of human toddlers) as a byproduct from a local slaughter house and very carefully prepared the samples. Tensile tests were performed on specimens at dynamic strain rates of 2/s, 20/s, and 100/s using a biological material instrument. The constitutive models, Fung, Ogden, Gent, and exponential function, for immature brainstem tissue material property were developed for the recorded experimental data using OriginPro 8.0 software. The t test was performed for infinitesimal shear modules. The curves of stress-versus-stretch ratio were convex in shape, and inflection points were found in all the test groups at the strain of about 2.5%. The average Lagrange stress of the immature brainstem specimen at the 30% strain at the strain rates of 2, 20, and 100/s was 273±114, 515±107, and 1121±197 Pa, respectively. The adjusted R-Square (R2) of Fung, Ogden, Gent, and exponential model was 0.820≤R2≤0.933, 0.774≤R2≤0.940, 0.650≤R2≤0.922, and 0.852≤R2≤0.981, respectively. The infinitesimal shear modulus of the strain energy functions showed a significant association with the strain rate (pmaterial in dynamic tensile tests, and the tissue becomes stiffer with increased strain rate. The reported results may be useful in the study of brain injuries in children who sustain injuries in road traffic accidents. Further research in more detail should be performed in the future.

Measurement of Plastic Stress and Strain for Analytical Method Verification (MSFC Center Director's Discretionary Fund Project No. 93-08)

Science.gov (United States)

Price, J. M.; Steeve, B. E.; Swanson, G. R.

1999-01-01

The analytical prediction of stress, strain, and fatigue life at locations experiencing local plasticity is full of uncertainties. Much of this uncertainty arises from the material models and their use in the numerical techniques used to solve plasticity problems. Experimental measurements of actual plastic strains would allow the validity of these models and solutions to be tested. This memorandum describes how experimental plastic residual strain measurements were used to verify the results of a thermally induced plastic fatigue failure analysis of a space shuttle main engine fuel pump component.

Pathological changes in the subsynovial connective tissue increase with self-reported carpal tunnel syndrome symptoms.

Science.gov (United States)

Tat, Jimmy; Wilson, Katherine E; Keir, Peter J

2015-05-01

Fibrosis and thickening of the subysnovial connective tissue are the most common pathological findings in carpal tunnel syndrome. The relationship between subsynovial connective tissue characteristics and self-reported carpal tunnel syndrome symptoms was assessed. Symptoms were characterized using the Boston Carpal Tunnel Questionnaire and Katz hand diagram in twenty-two participants (11 with symptoms, 11 with no symptoms). Using ultrasound, the thickness of the subsynovial connective tissue was measured using a thickness ratio (subsynovial thickness/tendon thickness) and gliding function was assessed using a shear strain index ((Displacement(tendon)-Displacement(subsynovial))/Displacement(tendon)x 100). For gliding function, participants performed 10 repeated flexion-extension cycles of the middle finger at a rate of one cycle per second. Participants with symptoms had a 38.5% greater thickness ratio and 39.2% greater shear strain index compared to participants without symptoms (p<0.05). Ultrasound detected differences the SSCT in symptomatic group that was characterized by low self-reported symptom severity scores. This study found ultrasound useful for measuring structural and functional changes in the SSCT that could provide insight in the early pathophysiology associated with carpal tunnel syndrome symptoms. Copyright © 2015 Elsevier Ltd. All rights reserved.

A strain gauge

DEFF Research Database (Denmark)

2017-01-01

The invention relates to a strain gauge of a carrier layer and a meandering measurement grid (101) positioned on the carrier layer, wherein the measurement grid comprises a number of measurement grid sections placed side by side with gaps in between, and a number of end loops (106) interconnecting...... relates to a method for manufacturing a strain gauge as mentioned above....

Phytohormone Involvement in the Ustilago maydis- Zea mays Pathosystem: Relationships between Abscisic Acid and Cytokinin Levels and Strain Virulence in Infected Cob Tissue.

Directory of Open Access Journals (Sweden)

Erin N Morrison

Full Text Available Ustilago maydis is the causative agent of common smut of corn. Early studies noted its ability to synthesize phytohormones and, more recently these growth promoting substances were confirmed as cytokinins (CKs. Cytokinins comprise a group of phytohormones commonly associated with actively dividing tissues. Lab analyses identified variation in virulence between U. maydis dikaryon and solopathogen infections of corn cob tissue. Samples from infected cob tissue were taken at sequential time points post infection and biochemical profiling was performed using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS. This hormone profiling revealed that there were altered levels of ABA and major CKs, with a marked reduction in CK glucosides, increases in methylthiol CKs and a particularly dramatic increase in cisZ CK forms, in U. maydis infected tissue. These changes were more pronounced in the more virulent dikaryon relative to the solopathogenic strain suggesting a role for cytokinins in moderating virulence during biotrophic infection. These findings highlight the fact that U. maydis does not simply mimic a fertilized seed but instead reprograms the host tissue. Results underscore the suitability of the Ustilago maydis- Zea mays model as a basis for investigating the control of phytohormone dynamics during biotrophic infection of plants.

Measurements of translation, rotation and strain: new approaches to seismic processing and inversion

NARCIS (Netherlands)

Bernauer, M.; Fichtner, A.; Igel, H.

2012-01-01

We propose a novel approach to seismic tomography based on the joint processing of translation, strain and rotation measurements. Our concept is based on the apparent S and P velocities, defined as the ratios of displacement velocity and rotation amplitude, and displacement velocity and

Strain fields around dislocation arrays in a Σ9 silicon bicrystal measured by scanning transmission electron microscopy

Science.gov (United States)

Couillard, Martin; Radtke, Guillaume; Botton, Gianluigi A.

2013-04-01

Strain fields around grain boundary dislocations are measured by applying geometric phase analysis on atomic resolution images obtained from multiple fast acquisitions in scanning transmission electron microscopy. Maps of lattice distortions in silicon introduced by an array of pure edge dislocations located at a Σ9(122) grain boundary are compared with the predictions from isotropic elastic theory, and the atomic structure of dislocation cores is deduced from images displaying all the atomic columns. For strain measurements, reducing the acquisition time is found to significantly decrease the effects of instabilities on the high-resolution images. Contributions from scanning artefacts are also diminished by summing multiple images following a cross-correlation alignment procedure. Combined with the sub-Ångström resolution obtained with an aberration corrector, and the stable dedicated microscope's environment, therapid acquisition method provides the measurements of atomic displacements with accuracy below 10 pm. Finally, the advantages of combining strain measurements with the collection of various analytical signals in a scanning transmission electron microscope are discussed.

Experimental technologies comparison for strain measurement of a composite main landing gear bay specimen

Science.gov (United States)

Viscardi, Massimo; Arena, Maurizio; Ciminello, Monica; Guida, Michele; Meola, Carosena; Cerreta, Pietro

2018-03-01

The development of advanced monitoring system for strain measurements on aeronautical components remain an important target both when related to the optimization of the lead-time and cost for part validation, allowing earlier entry into service, and when related to the implementation of advanced health monitoring systems dedicated to the in-service parameters verification and early stage detection of structural problems. The paper deals with the experimental testing of a composite samples set of the main landing gear bay for a CS-25 category aircraft, realized through an innovative design and production process. The test have represented a good opportunity for direct comparison of different strain measurement techniques: Strain Gauges (SG) and Fibers Bragg Grating (FBG) have been used as well as non-contact techniques, specifically the Digital Image Correlation (DIC) and Infrared (IR) thermography applied where possible in order to highlight possible hot-spot during the tests. The crucial points identification on the specimens has been supported by means of advanced finite element simulations, aimed to assessment of the structural strength and deformation as well as to ensure the best performance and the global safety of the whole experimental campaign.

Field Strain Measurement on the Fiber-Epoxy Scale in CFRPs

KAUST Repository

Tao, Ran

2015-06-08

Laminated composites are materials with complex architecture made of continuous fibers (usually glass or carbon) embedded within a polymeric resin. The properties of the raw materials can vary from one point to another due to different local processing conditions or complex geometrical features for example. A first step towards the identification of these spatially varying material parameters is to image with precision the displacement fields in this complex microstructure when subjected to mechanical loading. Secondary electron images obtained by scanning electron microscopy (SEM) and then numerically deformed are post-processed by either local subset-based digital image correlation (DIC) or global finite-element based DIC to measure the displacement and strain fields at the fiber-matrix scale in a cross-ply composite. It is shown that when global DIC is applied with a conformal mesh, it can capture more accurate local variations in the strain fields as it takes into account the underlying microstructure. In comparison to subset DIC, global DIC is better suited for capturing gradients across the fiber-matrix interfaces.

Optical coherence elastography assesses tissue modifications in laser reshaping of cornea and cartilages

Science.gov (United States)

Zaitsev, V. Y.; Matveyev, A. L.; Matveev, L. A.; Gelikonov, G. V.; Omelchenko, A. I.; Shabanov, D. V.; Sovetsky, A. A.; Baum, O. I.; Vitkin, A.; Sobol, E. N.

2018-02-01

Non-surgical thermo-mechanical reshaping of avascular collagenous tissues (cartilages and cornea) using moderate heating by IR-laser irradiation is an emerging technology that can find important applications in visioncorrection problems and preparation of cartilaginous implants in otolaryngology. To estimate both transient interframe strains and cumulative resultant strains produced by the laser irradiation of the tissue we use and improved version of strain mapping developed in our previous work related to compressional phase-sensitive optical coherence tomography. To reveal microstructural changes in the tissue regions where irradiation-produced strains do not disappear after temperature equilibration, we apply compressional optical coherence elastography in order to visualize the resultant variations in the tissue stiffness. The so-found regions of the stiffness reduction are attributed to formation of microscopic pores, existence of which agree with independent data obtained using methods of high-resolution microscopy.

Grain orientation and strain measurements in sub-micron wide passivated individual aluminum test structures

International Nuclear Information System (INIS)

Tamura, N.; Valek, B.C.; Spolenak, R.; MacDowell, A.A.; Celestre, R.S.; Padmore, H.A.; Brown, W.L.; Marieb, T.; Bravman, J.C.; Batterman, B.W.; Patel, J.R.

2001-01-01

An X-ray microdiffraction dedicated beamline, combining white and monochromatic beam capabilities, has been built at the Advanced Light Source. The purpose of this beamline is to address the myriad of problems in Materials Science and Physics that require submicron x-ray beams for structural characterization. Many such problems are found in the general area of thin films and nano-materials. For instance, the ability to characterize the orientation and strain state in individual grains of thin films allows us to measure structural changes at a very local level. These microstructural changes are influenced heavily by such parameters as deposition conditions and subsequent treatment. The accurate measurement of strain gradients at the micron and sub-micron level finds many applications ranging from the strain state under nano-indenters to gradients at crack tips. Undoubtedly many other applications will unfold in the future as we gain experience with the capabilities and limitations of this instrument. We have applied this technique to measure grain orientation and residual stress in single grains of pure Al interconnect lines and preliminary results on post-electromigration test experiments are presented. It is shown that measurements with this instrument can be used to resolve the complete stress tensor (6 components) in a submicron volume inside a single grain of Al under a passivation layer with an overall precision of about 20 MPa. The microstructure of passivated lines appears to be complex, with grains divided into identifiable subgrains and noticeable local variations of both tensile/compressive and shear stresses within single grains

Small RNA expression and strain specificity in the rat

Directory of Open Access Journals (Sweden)

de Bruijn Ewart

2010-04-01

Full Text Available Abstract Background Digital gene expression (DGE profiling has become an established tool to study RNA expression. Here, we provide an in-depth analysis of small RNA DGE profiles from two different rat strains (BN-Lx and SHR from six different rat tissues (spleen, liver, brain, testis, heart, kidney. We describe the expression patterns of known and novel micro (miRNAs and piwi-interacting (piRNAs. Results We confirmed the expression of 588 known miRNAs (54 in antisense orientation and identified 56 miRNAs homologous to known human or mouse miRNAs, as well as 45 new rat miRNAs. Furthermore, we confirmed specific A to I editing in brain for mir-376a/b/c and identified mir-377 as a novel editing target. In accordance with earlier findings, we observed a highly tissue-specific expression pattern for all tissues analyzed. The brain was found to express the highest number of tissue-specific miRNAs, followed by testis. Notably, our experiments also revealed robust strain-specific differential miRNA expression in the liver that is caused by genetic variation between the strains. Finally, we identified two types of germline-specific piRNAs in testis, mapping either to transposons or in strand-specific clusters. Conclusions Taken together, the small RNA compendium described here advances the annotation of small RNAs in the rat genome. Strain and tissue-specific expression patterns furthermore provide a strong basis for studying the role of small RNAs in regulatory networks as well as biological process like physiology and neurobiology that are extensively studied in this model system.

Development and Measurement of Strain Free RF Photoinjector Vacuum Windows

CERN Document Server

Biedron, Sandra G

2004-01-01

RF photoinjectors produce the highest brightness electron bunches only under nearly ideal illumination by a drive laser. The vacuum window used to introduce the laser beam is an essential element that may potentially degrade any distribution, making it difficult or impossible to know the actual uniformity achieved at the cathode. Because of the necessity to obtain ultrahigh vacuum near the photoinjector, some restrictions are imposed on the fabrication technology available to manufacture distortion-free windows. At the UV wavelengths commonly used for photoinjectors, it is challenging to measure and eliminate degradation caused by vacuum windows. Here, we discuss the initial laser-based measurements of a strain-free, coated, UHV window manufactured by Insulator Seal in collaboration with members of Brookhaven and Argonne National Laboratories.

Sensitive element of multifunctional sensor for measuring temperature, strain and magnetic field induction

Directory of Open Access Journals (Sweden)

Druzhinin A. A.

2017-12-01

Full Text Available Sensitive element of multifunctional sensor for measuring temperature, strain and magnetic field induction has been developed based on the studies of electrical conductivity and magnetoresistance of silicon and germanium microcrystals in the temperature range 4.2—70 K, strain ±1.5*10–3 rel.un. and magnetic fields of 0—14 T. The feature of the sensitive element is the using of the p- and n-type conductivity germanium microcrystals as mechanical and magnetic field sensors, respectively, and the p-type silicon microcrystal — as temperature sensor. That allows providing the compensation of temperature influence on piezoresistance and on sensitivity to the magnetic field.

Combined Synchrotron X-ray Diffraction and Digital Image Correlation Technique for Measurement of Austenite Transformation with Strain in TRIP-assisted Steels

Energy Technology Data Exchange (ETDEWEB)

Poling, Whitney A.; Savic, Vesna; Hector, Louis G.; Sachdev, Anil K.; Hu, Xiaohua; Devaraj, Arun; Abu-Farha, Fadi

2016-04-05

The strain-induced, diffusionless shear transformation of retained austenite to martensite during straining of transformation induced plasticity (TRIP) assisted steels increases strain hardening and delays necking and fracture leading to exceptional ductility and strength, which are attractive for automotive applications. A novel technique that provides the retained austenite volume fraction variation with strain in TRIP-assisted steels with improved precision is presented. Digital images of the gauge section of tensile specimens were first recorded up to selected plastic strains with a stereo digital image correlation (DIC) system. The austenite volume fraction was measured by synchrotron X-ray diffraction from small squares cut from the gage section. Strain fields in the squares were then computed by localizing the strain measurement to the corresponding region of a given square during DIC post-processing of the images recorded during tensile testing. Results obtained for a QP980 steel are used to study the influence of initial volume fraction of austenite and the austenite transformation with strain on tensile mechanical behavior.

Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.

OpenAIRE

Christensen, G D; Simpson, W A; Younger, J J; Baddour, L M; Barrett, F F; Melton, D M; Beachey, E H

1985-01-01

The adherence of coagulase-negative staphylococci to smooth surfaces was assayed by measuring the optical densities of stained bacterial films adherent to the floors of plastic tissue culture plates. The optical densities correlated with the weight of the adherent bacterial film (r = 0.906; P less than 0.01). The measurements also agreed with visual assessments of bacterial adherence to culture tubes, microtiter plates, and tissue culture plates. Selected clinical strains were passed through ...

Two-photon induced collagen cross-linking in bioartificial cardiac tissue

Science.gov (United States)

Kuetemeyer, Kai; Kensah, George; Heidrich, Marko; Meyer, Heiko; Martin, Ulrich; Gruh, Ina; Heisterkamp, Alexander

2011-08-01

Cardiac tissue engineering is a promising strategy for regenerative therapies to overcome the shortage of donor organs for transplantation. Besides contractile function, the stiffness of tissue engineered constructs is crucial to generate transplantable tissue surrogates with sufficient mechanical stability to withstand the high pressure present in the heart. Although several collagen cross-linking techniques have proven to be efficient in stabilizing biomaterials, they cannot be applied to cardiac tissue engineering, as cell death occurs in the treated area. Here, we present a novel method using femtosecond (fs) laser pulses to increase the stiffness of collagen-based tissue constructs without impairing cell viability. Raster scanning of the fs laser beam over riboflavin-treated tissue induced collagen cross-linking by two-photon photosensitized singlet oxygen production. One day post-irradiation, stress-strain measurements revealed increased tissue stiffness by around 40% being dependent on the fibroblast content in the tissue. At the same time, cells remained viable and fully functional as demonstrated by fluorescence imaging of cardiomyocyte mitochondrial activity and preservation of active contraction force. Our results indicate that two-photon induced collagen cross-linking has great potential for studying and improving artificially engineered tissue for regenerative therapies.

Measurement of minute local strain in semiconductor materials and electronic devices by using a highly parallel X-ray microbeam

CERN Document Server

Matsui, J; Yokoyama, K; Takeda, S; Katou, M; Kurihara, H; Watanabe, K; Kagoshima, Y; Kimura, S

2003-01-01

We have developed an X-ray microbeam with a small angular divergence by adopting X-ray optics with successive use of asymmetric Bragg reflection from silicon crystals for the both polarizations of the synchrotron X-rays. The microbeam actually obtained is several microns in size and possesses an angular divergence of less than 2 arcsec which enables us to measure the strain of 10 sup - sup 5 -10 sup - sup 6. By scanning the sample against the microbeam, distribution of the minute local strain in various regions of semiconductor crystals for electronic devices, e.g., the strain around the SiO sub 2 /Si film edge in silicon devices, the strain in an InGaAsP/InP stripe laser were measured.

Measurement of minute local strain in semiconductor materials and electronic devices by using a highly parallel X-ray microbeam

Energy Technology Data Exchange (ETDEWEB)

Matsui, J. E-mail: matsui@sci.himeji-tech.ac.jp; Tsusaka, Y.; Yokoyama, K.; Takeda, S.; Katou, M.; Kurihara, H.; Watanabe, K.; Kagoshima, Y.; Kimura, S

2003-01-01

We have developed an X-ray microbeam with a small angular divergence by adopting X-ray optics with successive use of asymmetric Bragg reflection from silicon crystals for the both polarizations of the synchrotron X-rays. The microbeam actually obtained is several microns in size and possesses an angular divergence of less than 2 arcsec which enables us to measure the strain of 10{sup -5}-10{sup -6}. By scanning the sample against the microbeam, distribution of the minute local strain in various regions of semiconductor crystals for electronic devices, e.g., the strain around the SiO{sub 2}/Si film edge in silicon devices, the strain in an InGaAsP/InP stripe laser were measured.

Dielectric property measurement of ocular tissues up to 110 GHz using 1 mm coaxial sensor

International Nuclear Information System (INIS)

Sasaki, K; Isimura, Y; Fujii, K; Wake, K; Watanabe, S; Kojima, M; Suga, R; Hashimoto, O

2015-01-01

Measurement of the dielectric properties of ocular tissues up to 110 GHz was performed by the coaxial probe method. A coaxial sensor was fabricated to allow the measurement of small amounts of biological tissues. Four-standard calibration was applied in the dielectric property measurement to obtain more accurate data than that obtained with conventional three-standard calibration, especially at high frequencies. Novel data of the dielectric properties of several ocular tissues are presented and compared with data from the de facto database. (paper)

The mechanical properties of human adipose tissues and their relationships to the structure and composition of the extracellular matrix.

Science.gov (United States)

Alkhouli, Nadia; Mansfield, Jessica; Green, Ellen; Bell, James; Knight, Beatrice; Liversedge, Neil; Tham, Ji Chung; Welbourn, Richard; Shore, Angela C; Kos, Katarina; Winlove, C Peter

2013-12-01

Adipose tissue (AT) expansion in obesity is characterized by cellular growth and continuous extracellular matrix (ECM) remodeling with increased fibrillar collagen deposition. It is hypothesized that the matrix can inhibit cellular expansion and lipid storage. Therefore, it is important to fully characterize the ECM's biomechanical properties and its interactions with cells. In this study, we characterize and compare the mechanical properties of human subcutaneous and omental tissues, which have different physiological functions. AT was obtained from 44 subjects undergoing surgery. Force/extension and stress/relaxation data were obtained. The effects of osmotic challenge were measured to investigate the cellular contribution to tissue mechanics. Tissue structure and its response to tensile strain were determined using nonlinear microscopy. AT showed nonlinear stress/strain characteristics of up to a 30% strain. Comparing paired subcutaneous and omental samples (n = 19), the moduli were lower in subcutaneous: initial 1.6 ± 0.8 (means ± SD) and 2.9 ± 1.5 kPa (P = 0.001), final 11.7 ± 6.4 and 32 ± 15.6 kPa (P matrix fibers. These results suggest that subcutaneous AT has greater capacity for expansion and recovery from mechanical deformation than omental AT.

Unified Hall-Petch description of nano-grain nickel hardness, flow stress and strain rate sensitivity measurements

Science.gov (United States)

Armstrong, R. W.; Balasubramanian, N.

2017-08-01

It is shown that: (i) nano-grain nickel flow stress and hardness data at ambient temperature follow a Hall-Petch (H-P) relation over a wide range of grain size; and (ii) accompanying flow stress and strain rate sensitivity measurements follow an analogous H-P relationship for the reciprocal "activation volume", (1/v*) = (1/A*b) where A* is activation area. Higher temperature flow stress measurements show a greater than expected reduction both in the H-P kɛ and in v*. The results are connected with smaller nano-grain size (tested at very low imposed strain rates.

Evaluation of left ventricular function by tissue Doppler and speckle-derived strain rate echocardiography after percutaneous ductus closure.

Science.gov (United States)

Amoogzar, Hamid; Shakiba, Ali Mohammad; Derakhshan, Dorna; Ajami, Gholamhossein; Cheriki, Sirous; Borzouee, Mohammad; Edraki, Mohammad Reza; Mehdizadegan, Nima

2015-01-01

The aim of this study was to evaluate the left ventricular systolic and diastolic function before and after transcatheter percutaneous patent ductus arteriosus (PDA) closure. 21 children (age >6 months old) diagnosed with hemodynamically significant PDA underwent percutaneous PDA closure. Conventional, Doppler and tissue Doppler imaging and speckled-derived strain rate echocardiography were done at pre-closure, 1 day (early) and 1 month (late) post-closure. Mean age of the patients (female/male: 1.3) was 17.54 ± 24.7 months with the mean PDA diameter of 3.6 ± 0.8 mm. Systolic measures (ejection fraction, shortening fraction) reduced significantly early after PDA closure (P closure status. Early and late diastolic flow velocities of mitral (E M and A M) reduced considerably in early and late post-closure time (P closure. After 1 month, E'M increased considerably. (P = 0.01) but E'M/A'M had an insignificant rise (P > 0.05). E M/E'M ratio did not change in early post-closure but it had a considerable reduction in the subsequent month compared with the pre- and early post-closure (P closure (P closure causes a significant decrease in left ventricular performance early after PDA closure which recovers completely within 1 month. Also PDA size can affect post-closure left ventricular function.

Modeling and Experimental Strain Measurements on a Non-Homogeneous Cylinder Under Transverse Load

National Research Council Canada - National Science Library

Viator, John A; Kreger, Stephen; Winz, Michele W; Udd, Eric

2004-01-01

...: core, cladding, and stress rods. We measure the strain on a multi-parameter ber Bragg grating written at 1550nm under transverse load at 0, 15, 30, 45, 60, 75, and 90 and compare these values with an analytical solution accounting for internal stresses and transverse load.

Cascaded-cavity Fabry-Perot interferometer for simultaneous measurement of temperature and strain with cross-sensitivity compensation

Science.gov (United States)

Tian, Jiajun; Jiao, Yuzhu; Ji, Shaobo; Dong, Xiaolong; Yao, Yong

2018-04-01

We propose and demonstrate a fiber sensor for simultaneous temperature and strain measurements. The proposed sensor is implemented by a cascaded-cavity Fabry-Perot (FP) fiber interferometer. The two cascaded FP cavities comprise a micro-air-cavity in a hollow-core tube fiber and a micro-silica-cavity in a standard single-mode fiber. To separate the interference spectrum of each FP cavity, the total spectrum is filtered in the frequency domain through band-pass filters, whose central frequencies were predesigned based on the relationship between the spatial frequency and free spectral range of each FP cavity. The different cross-sectional areas and thermal-optic coefficients of the two FP cavities confer different sensitivities to temperature and strain. Both parameters were measured simultaneously by tracking the wavelength shifts in the filtered interference spectra of the FP cavities. Moreover, the temperature-strain cross-sensitivity was compensated by solving a sensitivity-coefficient matrix equation for the two cavities, using the calibrated temperatures and strains. Other advantages of the proposed sensor are simple fabrication and an all-fiber structure. Owing to these properties, the proposed sensor is potentially applicable to real sensing applications.

Coronaviruses in brain tissue from patients with multiple sclerosis

DEFF Research Database (Denmark)

Dessau, R B; Lisby, G; Frederiksen, J L

2001-01-01

Brain tissue from 25 patients with clinically definite multiple sclerosis (MS) and as controls brain tissue from 36 patients without neurological disease was tested for the presence of human coronaviral RNA. Four PCR assays with primers specific for N-protein of human coronavirus strain 229E...... and three PCR assays with primers specific for the nucleocapsid protein of human coronavirus strain OC43 were performed. Sporadic positive PCR assays were observed in both patients and controls in some of the PCR assays. However, these results were not reproducible and there was no difference...... in the proportion of positive signals from the MS patients compared to controls. Evidence for a chronic infection with the human coronaviruses strain 229E or OC43 in brain tissue from patients with MS or controls has not been found in this study....

Electron density values of various human tissues: in vitro Compton scatter measurements and calculated ranges

International Nuclear Information System (INIS)

Shrimpton, P.C.

1981-01-01

Accurate direct measurements of electron density have been performed on specimens from 10 different tissue types of the human body, representing the major organs, using a Compton scatter technique. As a supplement to these experimental values, calculations have been carried out to determine the electron densities expected for these tissue types. The densities observed are in good agreement with the broad ranges deduced from the basic data previously published. The results of both the in vitro sample measurements and the approximate calculations indicate that the electron density of most normal healthy soft tissue can be expected to fall within the fairly restricted range of +- 5% around 3.4 X 10 23 electrons per cm 3 . The obvious exception to this generalisation is the result for lung tissue, which falls considerably below this range owing to the high air content inherent in its construction. In view of such an overall limited variation with little difference between tissues, it would appear that electron density alone is likely to be a rather poor clinical parameter for tissue analysis, with high accuracy and precision being essential in any in vivo Compton measurements for imaging or diagnosis on specific organs. (author)

Lung tissue mechanics as an emergent phenomenon.

Science.gov (United States)

Suki, Béla; Bates, Jason H T

2011-04-01

The mechanical properties of lung parenchymal tissue are both elastic and dissipative, as well as being highly nonlinear. These properties cannot be fully understood, however, in terms of the individual constituents of the tissue. Rather, the mechanical behavior of lung tissue emerges as a macroscopic phenomenon from the interactions of its microscopic components in a way that is neither intuitive nor easily understood. In this review, we first consider the quasi-static mechanical behavior of lung tissue and discuss computational models that show how smooth nonlinear stress-strain behavior can arise through a percolation-like process in which the sequential recruitment of collagen fibers with increasing strain causes them to progressively take over the load-bearing role from elastin. We also show how the concept of percolation can be used to link the pathologic progression of parenchymal disease at the micro scale to physiological symptoms at the macro scale. We then examine the dynamic mechanical behavior of lung tissue, which invokes the notion of tissue resistance. Although usually modeled phenomenologically in terms of collections of springs and dashpots, lung tissue viscoelasticity again can be seen to reflect various types of complex dynamic interactions at the molecular level. Finally, we discuss the inevitability of why lung tissue mechanics need to be complex.

Direct restoration modalities of fractured central maxillary incisors: A multi-levels validated finite elements analysis with in vivo strain measurements.

Science.gov (United States)

Davide, Apicella; Raffaella, Aversa; Marco, Tatullo; Michele, Simeone; Syed, Jamaluddin; Massimo, Marrelli; Marco, Ferrari; Antonio, Apicella

2015-12-01

To quantify the influence of fracture geometry and restorative materials rigidity on the stress intensity and distribution of restored fractured central maxillary incisors (CMI) with particular investigation of the adhesive interfaces. Ancillary objectives are to present an innovative technology to measure the in vivo strain state of sound maxillary incisors and to present the collected data. A validation experimental biomechanics approach has been associated to finite element analysis. FEA models consisted of CMI, periodontal ligament and the corresponding alveolar bone process. Three models were created representing different orientation of the fracture planes. Three different angulations of the fracture plane in buccal-palatal direction were modeled: the fracture plane perpendicular to the long axis in the buccal-palatal direction (0°); the fracture plane inclined bucco-palatally in apical-coronal direction (-30°); the fracture plane inclined palatal-buccally in apical-coronal direction (+30°). First set of computing runs was performed for in vivo FE-model validation purposes. In the second part, a 50N force was applied on the buccal aspect of the CMI models. Ten patients were selected and subjected to the strain measurement of CMI under controlled loading conditions. The main differences were noticed in the middle and incisal thirds of incisors crowns, due to the presence of the incisal portion restoration. The stress intensity in -30° models is increased in the enamel structure close to the restoration, due to a thinning of the remaining natural tissues. The rigidity of the restoring material slightly reduces such phenomenon. -30° model exhibits the higher interfacial stress in the adhesive layer with respect to +30° and 0° models. The lower stress intensity was noticed in the 0° models, restoration material rigidity did not influenced the interfacial stress state in 0° models. On the contrary, material rigidity influenced the interfacial stress state

Arthritis symptoms, the work environment, and the future: measuring perceived job strain among employed persons with arthritis.

Science.gov (United States)

Gignac, Monique A M; Sutton, Deborah; Badley, Elizabeth M

2007-06-15

To develop a measure of job strain related to differing aspects of working with arthritis and to examine the demographic, illness, work context, and psychosocial variables associated with it. Study participants were 292 employed individuals with osteoarthritis or inflammatory arthritis. Participants were from wave 3 of a 4-wave longitudinal study examining coping and adaptation efforts used to remain employed. Participants completed an interview-administered structured questionnaire, including a Chronic Illness Job Strain Scale (CIJSS) and questions on demographic (e.g., age, sex), illness and disability (e.g., disease type, pain, activity limitations), work context (e.g., job type, job control), and psychosocial variables (e.g., arthritis-work spillover, coworker/managerial support, job perceptions). Principal component analysis and multiple linear regression were used to analyze the data. A single factor solution emerged for the CIJSS. The scale had an internal reliability of 0.95. Greater job strain was reported for future uncertainty, balancing multiple roles, and difficulties accepting the disease than for current workplace conditions. Participants with inflammatory arthritis, more frequent severe pain, greater workplace activity limitations, fewer hours of work, less coworker support, and greater arthritis-work spillover reported greater job strain. The findings underscore the diverse areas that contribute to perceptions of job strain and suggest that existing models of job strain do not adequately capture the stress experienced by individuals working with chronic illnesses or the factors associated with job strain. Measures similar to the CIJSS can enhance the tools researchers and clinicians have available to examine the impact of arthritis in individuals' lives.

Residual strain in the Nb-H system measured by selected area diffraction (SAD)

International Nuclear Information System (INIS)

Bulhoes, I.A.M.; Akune, K.; Pinatti, Dyonisio G.

1981-07-01

Various specimens of Nb were annealed in vacuum of 10 -3 torr for four hours at 1770 0 K. These speciments were doped with hydrogen up to 1000 ppm by weight and then were analyzed selected area diffraction. The line resolution of the electron channelling pattern was meassured for the specimens with different hydrogen content. These measurements, combined with the measurement of density, permitted one to estimate the residual strain caused by hydrogen. (Author) [pt

Serum protein changes in immune and nonimmune pigeons infected with various strains of Trichomonas gallinae

Science.gov (United States)

Kocan, R.M.; Herman, C.M.