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Sample records for tissue sections electronic

  1. Imaging of tissue sections with very slow electrons

    Czech Academy of Sciences Publication Activity Database

    Frank, Luděk; Nebesářová, Jana; Vancová, Marie; Paták, Aleš; Müllerová, Ilona

    2015-01-01

    Roč. 148, JAN 2015 (2015), s. 146-150 ISSN 0304-3991 R&D Projects: GA TA ČR TE01020118; GA MŠk(CZ) LO1212 Institutional support: RVO:68081731 ; RVO:60077344 Keywords : Biological STEM * Ultralow energy STEM * Tissue sections * Cathode lens * Depolymerisation Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 2.874, year: 2015

  2. Imaging of tissue sections with very slow electrons

    Energy Technology Data Exchange (ETDEWEB)

    Frank, L., E-mail: ludek@isibrno.cz [Institute of Scientific Instruments AS CR, v.v.i., Královopolská 147, 61264 Brno (Czech Republic); Nebesářová, J.; Vancová, M. [Biology Centre AS CR, v.v.i., Branišovská 31, 37005 České Budějovice (Czech Republic); Paták, A.; Müllerová, I. [Institute of Scientific Instruments AS CR, v.v.i., Královopolská 147, 61264 Brno (Czech Republic)

    2015-01-15

    The examination of thin sections of tissues with electron microscopes is an indispensable tool. Being composed of light elements, samples of living matter illuminated with electrons at the usual high energies of tens or even hundreds of kiloelectronvolts provide very low image contrasts in transmission or scanning transmission electron microscopes. Therefore, heavy metal salts are added to the specimen during preparation procedures (post-fixation with osmium tetroxide or staining). However, these procedures can modify or obscure the ultrastructural details of cells. Here we show that the energy of electrons used for the scanned transmission imaging of tissue sections can be reduced to mere hundreds or even tens of electronvolts and can produce extremely high contrast even for samples free of any metal salts. We found that when biasing a sufficiently thin tissue section sample to a high negative potential in a scanning transmission electron microscope, thereby reducing the energy of the electrons landing on the sample, and collecting the transmitted electrons with a grounded detector, we obtain a high contrast revealing structure details not enhanced by heavy atoms. Moreover, bombardment with slow electrons sensitively depolymerises the resin in which the tissue is embedded, thereby enhancing the transmitted signal with no observable loss of structure details. The use of low-energy electrons requires ultrathin sections of a thickness of less than 10 nm, but their preparation is now possible. Ultralow energy STEM provides a tool enabling the observation of very thin biological samples without any staining. This method should also be advantageous for examination of 2D crystals, thin films of polymers, polymer blends, etc. - Highlights: • Sections of a thickness below 10 nm were imaged in STEM at hundreds and tens of eV. • Image contrast grows steeply with decreasing electron energy in the STEM. • Very slow electrons provide high contrast for samples free of

  3. Correlative light and immuno-electron microscopy of retinal tissue cryostat sections

    Science.gov (United States)

    Burgoyne, Thomas; Lane, Amelia; Laughlin, William E.; Cheetham, Michael E.

    2018-01-01

    Correlative light-electron microscopy (CLEM) is a powerful technique allowing localisation of specific macromolecules within fluorescence microscopy (FM) images to be mapped onto corresponding high-resolution electron microscopy (EM) images. Existing methods are applicable to limited sample types and are technically challenging. Here we describe novel methods to perform CLEM and immuno-electron microscopy (iEM) on cryostat sections utilising the popular FM embedding solution, optimal cutting temperature (OCT) compound. Utilising these approaches, we have (i) identified the same phagosomes by FM and EM in the retinal pigment epithelium (RPE) of retinal tissue (ii) shown the correct localisation of rhodopsin on photoreceptor outer segment disc like-structures in iPSC derived optic cups and (iii) identified a novel interaction between peroxisomes and melanosomes as well as phagosomes in the RPE. These data show that cryostat sections allow easy characterisation of target macromolecule localisation within tissue samples, thus providing a substantial improvement over many conventional methods that are limited to cultured cells. As OCT embedding is routinely used for FM this provides an easily accessible and robust method for further analysis of existing samples by high resolution EM. PMID:29315318

  4. Grinding and polishing instead of sectioning for the tissue samples with a graft: Implications for light and electron microscopy.

    Science.gov (United States)

    Mukhamadiyarov, Rinat A; Sevostyanova, Victoria V; Shishkova, Daria K; Nokhrin, Andrey V; Sidorova, Olga D; Kutikhin, Anton G

    2016-06-01

    A broad use of the graft replacement requires a detailed investigation of the host-graft interaction, including both histological examination and electron microscopy. A high quality sectioning of the host tissue with a graft seems to be complicated; in addition, it is difficult to examine the same tissue area by both of the mentioned microscopy techniques. To solve these problems, we developed a new technique of epoxy resin embedding with the further grinding, polishing, and staining. Graft-containing tissues prepared by grinding and polishing preserved their structure; however, sectioning frequently required the explantation of the graft and led to tissue disintegration. Moreover, stained samples prepared by grinding and polishing may then be assessed by both light microscopy and backscattered scanning electron microscopy. Therefore, grinding and polishing outperform sectioning when applied to the tissues with a graft. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Automation of 3D reconstruction of neural tissue from large volume of conventional serial section transmission electron micrographs.

    Science.gov (United States)

    Mishchenko, Yuriy

    2009-01-30

    We describe an approach for automation of the process of reconstruction of neural tissue from serial section transmission electron micrographs. Such reconstructions require 3D segmentation of individual neuronal processes (axons and dendrites) performed in densely packed neuropil. We first detect neuronal cell profiles in each image in a stack of serial micrographs with multi-scale ridge detector. Short breaks in detected boundaries are interpolated using anisotropic contour completion formulated in fuzzy-logic framework. Detected profiles from adjacent sections are linked together based on cues such as shape similarity and image texture. Thus obtained 3D segmentation is validated by human operators in computer-guided proofreading process. Our approach makes possible reconstructions of neural tissue at final rate of about 5 microm3/manh, as determined primarily by the speed of proofreading. To date we have applied this approach to reconstruct few blocks of neural tissue from different regions of rat brain totaling over 1000microm3, and used these to evaluate reconstruction speed, quality, error rates, and presence of ambiguous locations in neuropil ssTEM imaging data.

  6. Serial section scanning electron microscopy (S3EM) on silicon wafers for ultra-structural volume imaging of cells and tissues.

    Science.gov (United States)

    Horstmann, Heinz; Körber, Christoph; Sätzler, Kurt; Aydin, Daniel; Kuner, Thomas

    2012-01-01

    High resolution, three-dimensional (3D) representations of cellular ultrastructure are essential for structure function studies in all areas of cell biology. While limited subcellular volumes have been routinely examined using serial section transmission electron microscopy (ssTEM), complete ultrastructural reconstructions of large volumes, entire cells or even tissue are difficult to achieve using ssTEM. Here, we introduce a novel approach combining serial sectioning of tissue with scanning electron microscopy (SEM) using a conductive silicon wafer as a support. Ribbons containing hundreds of 35 nm thick sections can be generated and imaged on the wafer at a lateral pixel resolution of 3.7 nm by recording the backscattered electrons with the in-lens detector of the SEM. The resulting electron micrographs are qualitatively comparable to those obtained by conventional TEM. S(3)EM images of the same region of interest in consecutive sections can be used for 3D reconstructions of large structures. We demonstrate the potential of this approach by reconstructing a 31.7 µm(3) volume of a calyx of Held presynaptic terminal. The approach introduced here, Serial Section SEM (S(3)EM), for the first time provides the possibility to obtain 3D ultrastructure of large volumes with high resolution and to selectively and repetitively home in on structures of interest. S(3)EM accelerates process duration, is amenable to full automation and can be implemented with standard instrumentation.

  7. Serial section scanning electron microscopy (S3EM on silicon wafers for ultra-structural volume imaging of cells and tissues.

    Directory of Open Access Journals (Sweden)

    Heinz Horstmann

    Full Text Available High resolution, three-dimensional (3D representations of cellular ultrastructure are essential for structure function studies in all areas of cell biology. While limited subcellular volumes have been routinely examined using serial section transmission electron microscopy (ssTEM, complete ultrastructural reconstructions of large volumes, entire cells or even tissue are difficult to achieve using ssTEM. Here, we introduce a novel approach combining serial sectioning of tissue with scanning electron microscopy (SEM using a conductive silicon wafer as a support. Ribbons containing hundreds of 35 nm thick sections can be generated and imaged on the wafer at a lateral pixel resolution of 3.7 nm by recording the backscattered electrons with the in-lens detector of the SEM. The resulting electron micrographs are qualitatively comparable to those obtained by conventional TEM. S(3EM images of the same region of interest in consecutive sections can be used for 3D reconstructions of large structures. We demonstrate the potential of this approach by reconstructing a 31.7 µm(3 volume of a calyx of Held presynaptic terminal. The approach introduced here, Serial Section SEM (S(3EM, for the first time provides the possibility to obtain 3D ultrastructure of large volumes with high resolution and to selectively and repetitively home in on structures of interest. S(3EM accelerates process duration, is amenable to full automation and can be implemented with standard instrumentation.

  8. Ultrathin sectioning for electron microscopy

    DEFF Research Database (Denmark)

    Rostgaard, Jørgen; Qvortrup, K

    1989-01-01

    During an electron microscopical study of the localization of the nucleoside diphosphatase IDPase in Reissner's membrane of the inner ear, it was discovered that the distilled water in the knife trough produced an annoying artefact. It dissolved all the lead phosphate reaction product from...... the sections, and thus converted a positive phosphatase reactivity to a false negative one. The water in the knife trough had a pH of approximately 5.4. Calculations showed that this is an expected acidity, if CO2 in the air equilibrates with distilled water, and that there is 200,000 times more acid...

  9. Tissue-electronics interfaces: from implantable devices to engineered tissues

    Science.gov (United States)

    Feiner, Ron; Dvir, Tal

    2018-01-01

    Biomedical electronic devices are interfaced with the human body to extract precise medical data and to interfere with tissue function by providing electrical stimuli. In this Review, we outline physiologically and pathologically relevant tissue properties and processes that are important for designing implantable electronic devices. We summarize design principles for flexible and stretchable electronics that adapt to the mechanics of soft tissues, such as those including conducting polymers, liquid metal alloys, metallic buckling and meandering architectures. We further discuss technologies for inserting devices into the body in a minimally invasive manner and for eliminating them without further intervention. Finally, we introduce the concept of integrating electronic devices with biomaterials and cells, and we envision how such technologies may lead to the development of bionic organs for regenerative medicine.

  10. Electron attachment cross sections obtained from electron attachment spectroscopy

    International Nuclear Information System (INIS)

    Popp, P.; Baumbach, J.I.; Leonhardt, J.W.; Mothes, S.

    1988-01-01

    Electron capture detectors have a high sensitivity for substances with high thermal electron attachment cross sections. The electron attachment spectroscopy makes it possible to change the mean electron energy in such a way that the maximum for dissociative electron attachment is reached. Thus, best operation modes of the detection system as well as significant dependencies of electron attachment coefficients are available. Cross sections for electron attachment as a function of the electron energy are obtained with the knowledge of electron energy distribution functions from Boltzmann equation analysis by a special computer code. A disadvantage of this electron attachment spectroscopy is the superposition of space charge effects due to the decrease of the electron drift velocity with increasing mean electron energy. These influences are discussed. (author)

  11. Depth sectioning using electron energy loss spectroscopy

    International Nuclear Information System (INIS)

    D'Alfonso, A J; Findlay, S D; Allen, L J; Cosgriff, E C; Kirkland, A I; Nellist, P D; Oxley, M P

    2008-01-01

    The continued development of electron probe aberration correctors for scanning transmission electron microscopy has enabled finer electron probes, allowing atomic resolution column-by-column electron energy loss spectroscopy. Finer electron probes have also led to a decrease in the probe depth of focus, facilitating optical slicing or depth sectioning of samples. The inclusion of post specimen aberration corrected image forming lenses allows for scanning confocal electron microscopy with further improved depth resolution and selectivity. We show that in both scanning transmission electron microscopy and scanning confocal electron microscopy geometries, by performing a three dimensional raster scan through a specimen and detecting electrons scattered with a characteristic energy loss, it will be possible to determine the location of isolated impurities embedded within the bulk.

  12. Relativistic photon-Maxwellian electron cross sections

    International Nuclear Information System (INIS)

    Wienke, B.R.; Lathrop, B.L.; Devaney, J.J.

    1986-01-01

    Temperature corrected cross sections, complementing the Klein-Nishina set, are developed for astrophysical, plasma, and transport applications. The set is obtained from a nonlinear least squares fit to the exact photon-Maxwellian electron cross sections, using the static formula as the asymptotic basis. Two parameters are sufficient (two decimal places) to fit the exact cross sections over a range of 0-100 keV in electron temperature, and 0-1 MeV in incident photon energy. The fit is made to the total cross sections, yet the parameters predict both total and differential scattering cross sections well. Corresponding differential energy cross sections are less accurate. An extended fit to (just) the total cross sections, over the temperature and energy range 0-5 MeV, is also described. (author)

  13. Electron collision cross sections of mercury

    International Nuclear Information System (INIS)

    Suzuki, Susumu; Kuzuma, Kiyotaka; Itoh, Haruo

    2006-01-01

    In this paper, we propose a new collision cross section set for mercury which revises the original set summarized by Hayashi in 1989. Hanne reported three excitation collision cross sections (6 3 P 0 , 6 3 P 1 , 6 3 P 2 ) determined from an electron beam experiment in 1988. As a matter for regret, no attentive consideration was given to combining these three excitation cross sections with the cross section set of Hayashi. Therefore we propose a new set where these three excitation cross sections are included. In this study, other two excitation cross sections (6 1 P 1 , 6 3 D 3 ) except for the three excitation collision cross sections (6 3 P 0 , 6 3 P 1 , 6 3 P 2 ) are taken from the original set of Hayashi. The momentum transfer cross section and the ionization collision cross section are also taken from Hayashi. A Monte Carlo Simulation (MCS) technique is applied for evaluating our new cross section set. The present results of the electron drift velocity and the ionization coefficient are compared to experimental values. Agreement is secured in relation to the electron drift velocity for 1.5 Td 2 ) is the reduced electric field, E (V/cm) is the electric field, N (1/cm 3 ) is the number density of mercury atoms at 0degC, 1 Torr, E/N is also equal to 2.828 x 10 -17 E/p 0 from the relation of the ideal gas equation, p 0 (Torr) is gas pressure at 0degC, 1 Torr=1.33322 x 10 -2 N/cm -2 and 10 -17 V/cm 2 is called 1 Td. Thus it is ensured that our new cross section set is reasonable enough to be used up to 100 eV when considering with the electron drift velocity and the ionization coefficient. (author)

  14. Improved resolution by mounting of tissue sections for laser microdissection.

    NARCIS (Netherlands)

    Dijk, M.C.R.F. van; Rombout, P.D.M.; Dijkman, H.B.P.M.; Ruiter, D.J.; Bernsen, M.R.

    2003-01-01

    BACKGROUND: Laser microbeam microdissection has greatly facilitated the procurement of specific cell populations from tissue sections. However, the fact that a coverslip is not used means that the morphology of the tissue sections is often poor. AIMS: To develop a mounting method that greatly

  15. Rapid in vivo vertical tissue sectioning by multiphoton tomography

    Science.gov (United States)

    Batista, Ana; Breunig, Hans Georg; König, Karsten

    2018-02-01

    A conventional tool in the pathological field is histology which involves the analysis of thin sections of tissue in which specific cellular structures are stained with different dyes. The process to obtain these stained tissue sections is time consuming and invasive as it requires tissue removal, fixation, sectioning, and staining. Moreover, imaging of live tissue is not possible. We demonstrate that multiphoton tomography can provide within seconds, non-invasive, label-free, vertical images of live tissue which are in quality similar to conventional light micrographs of histologic stained specimen. In contrast to conventional setups based on laser scanning which image horizontally sections, the vertical in vivo images are directly recorded by combined line scanning and timed adjustments of the height of the focusing optics. In addition, multiphoton tomography provides autofluorescence lifetimes which can be used to determine the metabolic states of cells.

  16. Electron scattering cross sections pertinent to electron microscopy

    International Nuclear Information System (INIS)

    Inokuti, M.

    1978-01-01

    Some elements of the physics that determine cross sections are discussed, and various sources of data are indicated that should be useful for analytical microscopy. Atoms, molecules, and to some extent, solids are considered. Inelastic and elastic scattering of electrons and some solid-state effects are treated. 30 references

  17. Electron Capture Cross Sections for Stellar Nucleosynthesis

    Directory of Open Access Journals (Sweden)

    P. G. Giannaka

    2015-01-01

    Full Text Available In the first stage of this work, we perform detailed calculations for the cross sections of the electron capture on nuclei under laboratory conditions. Towards this aim we exploit the advantages of a refined version of the proton-neutron quasiparticle random-phase approximation (pn-QRPA and carry out state-by-state evaluations of the rates of exclusive processes that lead to any of the accessible transitions within the chosen model space. In the second stage of our present study, we translate the abovementioned e--capture cross sections to the stellar environment ones by inserting the temperature dependence through a Maxwell-Boltzmann distribution describing the stellar electron gas. As a concrete nuclear target we use the 66Zn isotope, which belongs to the iron group nuclei and plays prominent role in stellar nucleosynthesis at core collapse supernovae environment.

  18. Electron collision cross sections and radiation chemistry

    International Nuclear Information System (INIS)

    Hatano, Y.

    1983-01-01

    A survey is given of the cross section data needs in radiation chemistry, and of the recent progress in electron impact studies on dissociative excitation of molecules. In the former some of the important target species, processes, and collision energies are presented, while in the latter it is demonstrated that radiation chemistry is a source of new ideas and information in atomic collision research. 37 references, 4 figures

  19. Electron-impact cross sections of Ne

    International Nuclear Information System (INIS)

    Tsurubuchi, S.; Arakawa, K.; Kinokuni, S.; Motohashi, K.

    2000-01-01

    Electron-impact absolute emission cross sections were measured for the 3p→3s transitions of Ne. Excitation functions of the 3s→2p first resonance lines were measured in the energy range from the threshold to 1000 eV by a polarization-free optical method and relative cross sections were normalized to the absolute values, (41.0±5.4)x10 -19 cm 2 for the 73.6 nm line and (7.1±1.0)x10 -19 cm 2 for the 74.4 nm line, which were determined at 500 eV. The integrated level-excitation cross sections of Suzuki et al for the 1s 2 and 1s 4 levels were combined with the corresponding 3p→3s cascade cross sections obtained in this paper to give absolute emission cross sections for the resonance lines. The level-excitation cross sections of the 1s 2 and 1s 4 states in Paschen notation were determined from the threshold to 1000 eV by subtracting 3p→3s cascade cross sections from the corresponding 3s→2p emission cross sections of the resonance lines. A large cascade contribution is found in the emission cross section of the resonance lines. It is 28.5% for the 73.6 nm line and 49.6% for the 74.4 nm line at 40 eV, and 17.0 and 61.8%, respectively, at 300 eV. (author)

  20. Improved resolution by mounting of tissue sections for laser microdissection.

    Science.gov (United States)

    van Dijk, M C R F; Rombout, P D M; Dijkman, H B P M; Ruiter, D J; Bernsen, M R

    2003-08-01

    Laser microbeam microdissection has greatly facilitated the procurement of specific cell populations from tissue sections. However, the fact that a coverslip is not used means that the morphology of the tissue sections is often poor. To develop a mounting method that greatly improves the morphological quality of tissue sections for laser microbeam microdissection purposes so that the identification of target cells can be facilitated. Fresh frozen tissue and formalin fixed, paraffin wax embedded tissue specimens were used to test the morphological quality of mounted and unmounted tissue. The mounting solution consisted of an adhesive gum and blue ink diluted in water. Interference of the mounting solution with DNA quality was analysed by the polymerase chain reaction using 10-2000 cells isolated by microdissection from mounted and unmounted tissue. The mounting solution greatly improved the morphology of tissue sections for laser microdissection purposes and had no detrimental effects on the isolation and efficiency of amplification of DNA. One disadvantage was that the mounting solution reduced the cutting efficiency of the ultraviolet laser. To minimise this effect, the mounting solution should be diluted as much as possible. Furthermore, the addition of blue ink to the mounting medium restores the cutting efficiency of the laser. The mounting solution is easy to prepare and apply and can be combined with various staining methods without compromising the quality of the DNA extracted.

  1. Electron-collision cross sections for iodine

    International Nuclear Information System (INIS)

    Zatsarinny, O.; Bartschat, K.; Garcia, G.; Blanco, F.; Hargreaves, L.R.; Jones, D.B.; Murrie, R.; Brunton, J.R.; Brunger, M.J.; Hoshino, M.; Buckman, S.J.

    2011-01-01

    We present results from a joint experimental and theoretical study of elastic electron scattering from atomic iodine. The experimental results were obtained by subtracting known cross sections from the measured data obtained with a pyrolyzed mixed beam containing a variety of atomic and molecular species. The calculations were performed using both a fully relativistic Dirac B-spline R-matrix (close-coupling) method and an optical model potential approach. Given the difficulty of the problem, the agreement between the two sets of theoretical predictions and the experimental data for the angle-differential and the angle-integrated elastic cross sections at 40 eV and 50 eV is satisfactory.

  2. Evaporation process in histological tissue sections for neutron autoradiography.

    Science.gov (United States)

    Espector, Natalia M; Portu, Agustina; Santa Cruz, Gustavo A; Saint Martin, Gisela

    2018-05-01

    The analysis of the distribution and density of nuclear tracks forming an autoradiography in a nuclear track detector (NTD) allows the determination of 10 B atoms concentration and location in tissue samples from Boron Neutron Capture Therapy (BNCT) protocols. This knowledge is of great importance for BNCT dosimetry and treatment planning. Tissue sections studied with this technique are obtained by cryosectioning frozen tissue specimens. After the slicing procedure, the tissue section is put on the NTD and the sample starts drying. The thickness varies from its original value allowing more particles to reach the detector and, as the mass of the sample decreases, the boron concentration in the sample increases. So in order to determine the concentration present in the hydrated tissue, the application of corrective coefficients is required. Evaporation mechanisms as well as various factors that could affect the process of mass variation are outlined in this work. Mass evolution for tissue samples coming from BDIX rats was registered with a semimicro analytical scale and measurements were analyzed with software developed to that end. Ambient conditions were simultaneously recorded, obtaining reproducible evaporation curves. Mathematical models found in the literature were applied for the first time to this type of samples and the best fit of the experimental data was determined. The correlation coefficients and the variability of the parameters were evaluated, pointing to Page's model as the one that best represented the evaporation curves. These studies will contribute to a more precise assessment of boron concentration in tissue samples by the Neutron Autoradiography technique.

  3. Raman molecular imaging of brain frozen tissue sections.

    Science.gov (United States)

    Kast, Rachel E; Auner, Gregory W; Rosenblum, Mark L; Mikkelsen, Tom; Yurgelevic, Sally M; Raghunathan, Aditya; Poisson, Laila M; Kalkanis, Steven N

    2014-10-01

    Raman spectroscopy provides a molecular signature of the region being studied. It is ideal for neurosurgical applications because it is non-destructive, label-free, not impacted by water concentration, and can map an entire region of tissue. The objective of this paper is to demonstrate the meaningful spatial molecular information provided by Raman spectroscopy for identification of regions of normal brain, necrosis, diffusely infiltrating glioma and solid glioblastoma (GBM). Five frozen section tissues (1 normal, 1 necrotic, 1 GBM, and 2 infiltrating glioma) were mapped in their entirety using a 300-µm-square step size. Smaller regions of interest were also mapped using a 25-µm step size. The relative concentrations of relevant biomolecules were mapped across all tissues and compared with adjacent hematoxylin and eosin-stained sections, allowing identification of normal, GBM, and necrotic regions. Raman peaks and peak ratios mapped included 1003, 1313, 1431, 1585, and 1659 cm(-1). Tissue maps identified boundaries of grey and white matter, necrosis, GBM, and infiltrating tumor. Complementary information, including relative concentration of lipids, protein, nucleic acid, and hemoglobin, was presented in a manner which can be easily adapted for in vivo tissue mapping. Raman spectroscopy can successfully provide label-free imaging of tissue characteristics with high accuracy. It can be translated to a surgical or laboratory tool for rapid, non-destructive imaging of tumor margins.

  4. New methods for multimodal MS imaging of histological tissue sections

    NARCIS (Netherlands)

    Amstalden Van Hove, E.R.

    2011-01-01

    The insights derived from spatial localization of molecules in tissue sections are of great value for understanding and treating cancer and other diseases. These insights can relate to molecules linked to a disease as well as to drug molecules distributed across organs of interest. Mass spectrometry

  5. Glycomic profiling of tissue sections by LC-MS.

    Science.gov (United States)

    Hu, Yunli; Zhou, Shiyue; Khalil, Sarah I; Renteria, Calvin L; Mechref, Yehia

    2013-04-16

    Because routine preparation of glycan samples involves multiple reaction and cleaning steps at which sample loss occurs, glycan analysis is typically performed using large tissue samples. This type of analysis yields no detailed molecular spatial information and requires special care to maintain proper storage and shipping conditions. We describe here a new glycan sample preparation protocol using minimized sample preparation steps and optimized procedures. Tissue sections and spotted samples first undergo on-surface enzymatic digestion to release N-glycans. The released glycans are then reduced and permethylated prior to online purification and LC-electrospray ionization (ESI)-MS analysis. The efficiency of this protocol was initially evaluated using model glycoproteins and human blood serum (HBS) spotted on glass or Teflon slides. The new protocol permitted the detection of permethylated N-glycans derived from 10 ng RNase B. On the other hand, 66 N-glycans were identified when injecting the equivalent of permethylated glycans derived from a 0.1-μL aliquot of HBS. On-tissue enzymatic digestion of nude mouse brain tissue permitted the detection of 43 N-glycans. The relative peak areas of these 43 glycans were comparable to those from a C57BL/6 mouse reported by the Consortium for Functional Glycomics (CFG). However, the sample size analyzed in the protocol described here was substantially smaller than for the routine method (submicrogram vs mg). The on-tissue N-glycan profiling method permits high sensitivity and reproducibility and can be widely applied to assess the spatial distribution of glycans associated with tissue sections, and may be correlated with immunoflourescence imaging when adjacent tissue sections are analyzed.

  6. Localization of xanthine oxidoreductase activity using the tissue protectant polyvinyl alcohol and final electron acceptor Tetranitro BT

    NARCIS (Netherlands)

    Kooij, A.; Frederiks, W. M.; Gossrau, R.; van Noorden, C. J.

    1991-01-01

    We have detected xanthine oxidoreductase activity in unfixed cryostat sections of rat and chicken liver, rat duodenum, and bovine mammary gland using the tissue protectant polyvinyl alcohol, the electron carrier 1-methoxyphenazine methosulfate, the final electron acceptor Tetranitro BT, and

  7. DNA Measurement of Overlapping Cell Nuclei in Thick Tissue Sections

    Directory of Open Access Journals (Sweden)

    Liang Ji

    1997-01-01

    Full Text Available The paper describes an improved image analysis procedure for measuring the DNA content of cell nuclei in thick sections of liver tissue by absorption densitometry. Whereas previous methods only permitted the analysis of isolated nuclei, the new technique enables both isolated and overlapping nuclei to be measured. A 3D segmentation procedure determines whether each object is an isolated nucleus or a pair of overlapping nuclei; in the latter case the combined optical density is redistributed to the individual nuclei. A selection procedure ensures that only complete nuclei are measured.

  8. Compact fitting formulas for electron-impact cross sections

    International Nuclear Information System (INIS)

    Kim, Y.K.

    1992-01-01

    Compact fitting formulas, which contain four fitting constants, are presented for electron-impact excitation and ionization cross sections of atoms and ions. These formulas can fit experimental and theoretical cross sections remarkably well, when resonant structures are smoothed out, from threshold to high incident electron energies (<10 keV), beyond which relativistic formulas are more appropriate. Examples of fitted cross sections for some atoms and ions are presented. The basic form of the formula is valid for both atoms and molecules

  9. Cross sections for collisions of subexcitation electrons with molecules

    International Nuclear Information System (INIS)

    Itikawa, Y.

    1983-01-01

    A short review is given on the present knowledge about the collision processes of subexcitation electrons (i.e., electrons whose energy is below the threshold of the first electronically excited state) with molecules. The processes considered are: vibrational and rotational excitations and elastic scattering. As an example, stopping cross sections for those processes (and electronic excitation and ionization, for comparison) are shown graphically for N 2 and H 2 O. 21 references, 2 figures

  10. Staining plastic blocks with triiodide to image cells and soft tissues in backscattered electron SEM of skeletal and dental tissues

    Directory of Open Access Journals (Sweden)

    A Boyde

    2012-07-01

    Full Text Available Backscattered electron scanning electron microscopy (BSE SEM is an invaluable method for studying the histology of the hard, mineralised components of poly-methyl methacrylate (PMMA or other resin embedded skeletal and dental tissues. Intact tissues are studied in micro-milled or polished block faces with an electron-optical section thickness of the order of a half to one micron and with the area of the section as big as a whole – large or small – bone organ. However, BSE SEM does not give information concerning the distribution of uncalcified, ‘soft’, cellular and extracellular matrix components. This can be obtained by confocal microscopy of the same block and the two sorts of images merged but the blocks have to be studied in two microscope systems. The present work shows a new, simple and economic approach to visualising both components by using the triiodide ion in Lugol's iodine solution to stain the block surface prior to the application of any conductive coating – and the latter can be omitted if charging is suppressed by use of poor vacuum conditions in the SEM sample chamber. The method permits the use of archival tissue, and it will be valuable in studies of both normal growth and development and pathological changes in bones and joints, including osteoporosis and osteoarthritis, and tissue adaptation to implants.

  11. Electron collision cross section sets of TMS and TEOS vapours

    Science.gov (United States)

    Kawaguchi, S.; Takahashi, K.; Satoh, K.; Itoh, H.

    2017-05-01

    Reliable and detailed sets of electron collision cross sections for tetramethylsilane [TMS, Si(CH3)4] and tetraethoxysilane [TEOS, Si(OC2H5)4] vapours are proposed. The cross section sets of TMS and TEOS vapours include 16 and 20 kinds of partial ionization cross sections, respectively. Electron transport coefficients, such as electron drift velocity, ionization coefficient, and longitudinal diffusion coefficient, in those vapours are calculated by Monte Carlo simulations using the proposed cross section sets, and the validity of the sets is confirmed by comparing the calculated values of those transport coefficients with measured data. Furthermore, the calculated values of the ionization coefficient in TEOS/O2 mixtures are compared with measured data to confirm the validity of the proposed cross section set.

  12. Section I: Basic Electricity. Syllabus in Trade Electricity-Electronics.

    Science.gov (United States)

    New York State Education Dept., Albany. Bureau of Occupational and Career Curriculum Development.

    This section describes the first of a three part curriculum in trade electricity-electronics (each part is described in a separate volume). It presents a unit of 6 to 10 weeks duration which develops only those competencies necessary to all electricity or electronics employment. A flow chart indicates how an individual student's program can be…

  13. Applications of cross sections for electron-molecule collision processes

    International Nuclear Information System (INIS)

    Cartwright, D.C.

    1985-01-01

    The role of electron-molecule collision cross sections is discussed for the study of the ionospheric and auroral processes in planetary atmospheres and of discharge-pumped lasers. These two areas emphasize the importance of further theoretical and experimental studies concerning electron-impact processes. 13 refs., 3 figs., 2 tabs

  14. Syllabus in Trade Electricity-Electronics. Section II. Trade Electricity.

    Science.gov (United States)

    New York State Education Dept., Albany. Bureau of Occupational Education Curriculum Development.

    This second section of a three-part syllabus for a flexible curriculum in trade electricity-electronics contains four semi-independent units: (1) Advanced Electricity, (2) Residential and Commercial Wiring, (3) Industrial Electricity, and (4) Motor Controls. Introductory sections describe development of the curriculum, outline the total trade…

  15. Discussion of electron cross sections for transport calculations

    International Nuclear Information System (INIS)

    Berger, M.J.

    1983-01-01

    This paper deals with selected aspects of the cross sections needed as input for transport calculations and for the modeling of radiation effects in biological materials. Attention is centered mainly on the cross sections for inelastic interactions between electrons and water molecules and the use of these cross sections for the calculation of energy degradation spectra and of ionization and excitation yields. 40 references, 3 figures, 1 table

  16. Mesh Nanoelectronics: Seamless Integration of Electronics with Tissues.

    Science.gov (United States)

    Dai, Xiaochuan; Hong, Guosong; Gao, Teng; Lieber, Charles M

    2018-02-20

    Nanobioelectronics represents a rapidly developing field with broad-ranging opportunities in fundamental biological sciences, biotechnology, and medicine. Despite this potential, seamless integration of electronics has been difficult due to fundamental mismatches, including size and mechanical properties, between the elements of the electronic and living biological systems. In this Account, we discuss the concept, development, key demonstrations, and future opportunities of mesh nanoelectronics as a general paradigm for seamless integration of electronics within synthetic tissues and live animals. We first describe the design and realization of hybrid synthetic tissues that are innervated in three dimensions (3D) with mesh nanoelectronics where the mesh serves as both as a tissue scaffold and as a platform of addressable electronic devices for monitoring and manipulating tissue behavior. Specific examples of tissue/nanoelectronic mesh hybrids highlighted include 3D neural tissue, cardiac patches, and vascular constructs, where the nanoelectronic devices have been used to carry out real-time 3D recording of electrophysiological and chemical signals in the tissues. This novel platform was also exploited for time-dependent 3D spatiotemporal mapping of cardiac tissue action potentials during cell culture and tissue maturation as well as in response to injection of pharmacological agents. The extension to simultaneous real-time monitoring and active control of tissue behavior is further discussed for multifunctional mesh nanoelectronics incorporating both recording and stimulation devices, providing the unique capability of bidirectional interfaces to cardiac tissue. In the case of live animals, new challenges must be addressed, including minimally invasive implantation, absence of deleterious chronic tissue response, and long-term capability for monitoring and modulating tissue activity. We discuss each of these topics in the context of implantation of mesh

  17. Total and ionization cross sections of electron scattering by fluorocarbons

    International Nuclear Information System (INIS)

    Antony, B K; Joshipura, K N; Mason, N J

    2005-01-01

    Electron impact total cross sections (50-2000 eV) and total ionization cross sections (threshold to 2000 eV) are calculated for typical plasma etching molecules CF 4 , C 2 F 4 , C 2 F 6 , C 3 F 8 and CF 3 I and the CF x (x 1-3) radicals. The total elastic and inelastic cross sections are determined in the spherical complex potential formalism. The sum of the two gives the total cross section and the total inelastic cross section is used to calculate the total ionization cross sections. The present total and ionization cross sections are found to be consistent with other theories and experimental measurements, where they exist. Our total cross section results for CF x (x = 1-3) radicals presented here are first estimates on these species

  18. Electron collision cross sections and electron swarm parameters for gas molecules

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    1986-01-01

    Elastic and inelastic electron collision cross sections for molecules which interest in radiation research have been determined from available electron beam and electron swarm data by utilizing the Boltzmann equation and Monte Carlo simulation method. Electron drift velocity data which interest in radiation Pysics have been discussed and some calculated results of electron drift velocity data for CF 4 and CF 4 -inert gases mixtures will be presented. (author)

  19. [Scanning electron microscopy of heat-damaged bone tissue].

    Science.gov (United States)

    Harsanyl, L

    1977-02-01

    Parts of diaphyses of bones were exposed to high temperature of 200-1300 degrees C. Damage to the bone tissue caused by the heat was investigated. The scanning electron microscopic picture seems to be characteristic of the temperature applied. When the bones heated to the high temperature of 700 degrees C characteristic changes appear on the periostal surface, higher temperatura on the other hand causes damage to the compact bone tissue and can be observed on the fracture-surface. Author stresses the importance of this technique in the legal medicine and anthropology.

  20. Cross Sections for Inner-Shell Ionization by Electron Impact

    Energy Technology Data Exchange (ETDEWEB)

    Llovet, Xavier, E-mail: xavier@ccit.ub.edu [Centres Científics i Tecnològics, Universitat de Barcelona, Lluís Solé i Sabarís 1-3, 08028 Barcelona (Spain); Powell, Cedric J. [Materials Measurement Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8370 (United States); Salvat, Francesc [Facultat de Física (ECM and ICC), Universitat de Barcelona, Diagonal 645, 08028 Barcelona (Spain); Jablonski, Aleksander [Institute of Physical Chemistry, Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw (Poland)

    2014-03-15

    An analysis is presented of measured and calculated cross sections for inner-shell ionization by electron impact. We describe the essentials of classical and semiclassical models and of quantum approximations for computing ionization cross sections. The emphasis is on the recent formulation of the distorted-wave Born approximation by Bote and Salvat [Phys. Rev. A 77, 042701 (2008)] that has been used to generate an extensive database of cross sections for the ionization of the K shell and the L and M subshells of all elements from hydrogen to einsteinium (Z = 1 to Z = 99) by electrons and positrons with kinetic energies up to 1 GeV. We describe a systematic method for evaluating cross sections for emission of x rays and Auger electrons based on atomic transition probabilities from the Evaluated Atomic Data Library of Perkins et al. [Lawrence Livermore National Laboratory, UCRL-ID-50400, 1991]. We made an extensive comparison of measured K-shell, L-subshell, and M-subshell ionization cross sections and of Lα x-ray production cross sections with the corresponding calculated cross sections. We identified elements for which there were at least three (for K shells) or two (for L and M subshells) mutually consistent sets of cross-section measurements and for which the cross sections varied with energy as expected by theory. The overall average root-mean-square deviation between the measured and calculated cross sections was 10.9% and the overall average deviation was −2.5%. This degree of agreement between measured and calculated ionization and x-ray production cross sections was considered to be very satisfactory given the difficulties of these measurements.

  1. Total cross sections for positron and electron scattering from pyrimidine

    International Nuclear Information System (INIS)

    Zecca, A; Chiari, L; Trainotti, E; GarcIa, G; Blanco, F; Brunger, M J

    2010-01-01

    In this paper we report original measurements of total cross sections for positron scattering from the important biomolecule pyrimidine. The energy range of these measurements was 0.3-45 eV, while the energy resolution was ∼260 meV. In addition, we report theoretical results, calculated within the independent atom-screened additivity rule (IAM-SCAR) formalism, for the corresponding electron impact total cross sections. In that case the energy range is 1-10 000 eV. Total cross sections are very important input data for codes that seek to simulate charged-particle tracks in matter, as they define the mean-free path between collisions. As the present data and computations are to the best of our knowledge the first total cross sections to be reported for either positron or electron scattering from pyrimidine, they fill an important void in our available knowledge in the literature.

  2. Triply differential cross sections for ionization of helium by electrons

    International Nuclear Information System (INIS)

    Brauner, M.; Briggs, J.S.; Broad, J.T.

    1991-01-01

    A correlated three-body continuum wavefunction, already successfully employed to describe hydrogen atom impact ionization, is used to calculate the triply-differential cross section for electron impact ionization of helium. A good description is obtained of all the major structure in the differential cross sections in both symmetric and asymmetric geometries. It is demonstrated how interference between the various projectile-target interactions is necessary to reproduce the experimentally observed structure. (author)

  3. Measurements of the electron and muon inclusive cross-sections

    Indian Academy of Sciences (India)

    We present the measurements of the differential cross-sections for inclusive electron and muon production in proton–proton collisions at a centre-of-mass energy of s = 7 TeV, using ∼ 1.4 pb-1 of data collected by the ATLAS detector at the Large Hadron Collider. The muon cross-section is measured as a function of muon ...

  4. Total cross sections for electron scattering by He

    International Nuclear Information System (INIS)

    De Heer, F.J.; Jansen, R.H.J.

    1977-01-01

    A set of total cross sections for scattering of electrons by He has been evaluated over the energy range of zero to 3000 eV by means of the analysis of experiments and theories on total cross sections for elastic scattering, ionisation and excitation, and on differential cross sections for elastic and inelastic scattering. Between 0 and 19.8 eV, where no inelastic processes occur, the total cross sections for scattering are equal to those for elastic scattering. Above 19.8 eV total cross sections for scattering of electrons have been evaluated by adding those for ionisation, excitation and elastic scattering. The total cross sections thus obtained are probably accurate to about 5% over a large part of the energy range. They appear to be in very good agreement with the recent experimental results of Blaauw et al. (J. Phys. B.; 10:L299 (1977)). The present results have already proved useful for application in the dispersion relation for forward scattering in electron-helium collisions. (author)

  5. Electron collision cross sections and transport parameters in Cl2

    International Nuclear Information System (INIS)

    Pinhao, N.; Chouki, A.

    1995-01-01

    Molecular chlorine, Cl 2 , is a widely used gas with important application in gas discharge physics, namely in plasma etching, UV lasers and gas-filled particle detectors. However, due to experimental difficulties and to a complicated electronic spectrum, only some of the electron collision cross section have been measured and only recently the electronic structure has been resolved. This situation hampered the theoretical analysis of chlorine mixtures by a lack of relevant transport parameters. To our best knowledge there is only one published measurement of electron drift velocity and characteristic energy. Regrettably these data are considered of doubtful quality. There is also only one measurement of attachment and ionisation coefficients and one published set of cross sections. However those authors used the transport data from a He-Cl 2 mixture (80/20) where chlorine's effect can be hidden by the other component. Consequently that set is not completely consistent with the measurements in pure chlorine. This paper presents a new proposal of a consistent set of electron collision cross sections and the corresponding transport parameters and collision frequencies

  6. Near threshold electron impact ionization cross section for tellurium atoms

    International Nuclear Information System (INIS)

    Chipev, F.F.; Chernyshova, I.V.; Kontros, J.E.; Shpenik, O.B.

    2004-01-01

    Full text: Up today electron-impact ionization is one of the most intensively investigated processes in atomic and molecular physics [1]. These experiments however, are associated with difficulties: high temperatures and densities are required to produce atomic beams and monochromatic intensive electron beams. A crossed electron and atomic beams scattering geometry was employed to measure the ionization efficiency curve for tellurium atoms. Our electron spectrometer comprises two serially mounted hypocycloidal electron energy analyzers [2], the first being the monochromator and the second - the scattered electron analyzer. The whole spectrometer is immersed into the homogenous magnetic field. Great care was taken in selecting the value of the extracting potential at the electrode, mounted normally to the atomic beam direction. By careful choosing this potential as low as possible (∼1.4 V), its influence on the motion of the monochromatized electrons in the collision region was minimized and the full collection of the formed ions was reached. The atom beam was produced using a compact effusion source made of the stainless steel with a microchannel exit to minimise the angular divergency of the beam. The temperature of the microchannel plate was taken about 50 K higher than that of the metal vapour in the heated reservoir. This atomic beam source enabled to produce an atomic beam with the concentration of two orders of magnitude higher than that in the case of a standard effusion source. A typical value of the electron energy spread was 0.15 eV (FWHM) in the 0.1-15 eV energy range. The primary electron beam current was equal to 10 -7 A. Such values of electron energy spread and beam current for the primary electron beam passing through the collision chamber were chosen to provide identical conditions for carrying out all the measurements. The energy scale was calibrated with the accuracy of ± 0.05 eV. The measured ionization cross-section normalized to the results

  7. Electronic properties of carbon nanotubes with polygonized cross sections

    International Nuclear Information System (INIS)

    Charlier, J.; Lambin, P.; Ebbesen, T.

    1996-01-01

    The electronic properties of carbon nanotubes having polygonized cross sections instead of purely circular ones, such as recently observed using transmission electron microscopy, are investigated with plane-wave ab initio pseudopotential local-density-functional calculations and simple tight-binding models. Strong σ * -π * hybridization effects occur in zigzag nanotubes due to the high curvature located near the edges of the polygonal cross-section prism. These effects, combined with a lowering of symmetry, dramatically affect the electronic properties of the nanotubes. It is found that modified low-lying conduction-band states are introduced either into the bandgap of insulating nanotubes, or below the degenerate states that form the top of the valence band of metallic nanotubes, leading the corresponding nanostructures to be metals, semimetals, or at least very-small-gap semiconductors. The degree of the polygon representing the cross section of the tube, and the sharpness of the edge angles, are found to be major factors in the hybridization effect, and consequently govern the electronic behavior at the Fermi level. copyright 1996 The American Physical Society

  8. Electron and positron atomic elastic scattering cross sections

    International Nuclear Information System (INIS)

    Stepanek, Jiri

    2003-01-01

    A method was developed to calculate the total and differential elastic-scattering cross sections for incident electrons and positrons in the energy range from 0.01 eV to 1 MeV for atoms of Z=1-100. For electrons, hydrogen, helium, nitrogen, oxygen, krypton, and xenon, and for positrons, helium, neon, and argon atoms were considered for comparison with experimental data. First, the variationally optimized atomic static potentials were calculated for each atom by solving the Dirac equations for bound electron states. Second, the Dirac equations for a free electron or positron are solved for an atom using the previously calculated static potential accomplished (in the case of electrons) by 'adjusted' Hara's exchange potential for a free-state particle. Additional to the exchange effects, the charge cloud polarization effects are considered applying the correlation-polarization potential of O'Connell and Lane (with correction of Padial and Norcross) for incident electrons, and of Jain for incident positrons. The total, cutoff and differential elastic-scattering cross sections are calculated for incident electrons and positrons with the help of the relativistic partial wave analysis. The solid state effects for scattering in solids are described by means of a muffin-tin model, i.e. the potentials of neighboring atoms are superpositioned in such a way that the resulting potential and its derivative are zero in the middle distance between the atoms. The potential of isolated atom is calculated up to the radius at which the long-range polarization potential becomes a value of -10 -8

  9. Electron-collision excitation cross section of the silver atom

    International Nuclear Information System (INIS)

    Krasavin, A.Y.; Kuchenev, A.N.; Smirnov, Y.M.

    1983-01-01

    The cross sections for direct excitation by electron collision were measured for fifteen transitions of the silver atom. For thirteen of these transitions the optical excitation functions were recorded, varying the energy of the exciting electrons from the threshold energy to 250 eV. The operating region of the spectrum was 2000--5500 A. The excitation cross sections of the two principal lines exceeded the excitation cross sections of all the remaining lines by more than an order of magnitude. Reabsorption of the resonance lines was detected from the change in the ratio of intensities of the lines at 3280.68 and 3382.89 A, and so their intensity has been corrected relative to the intensities of the nonreabsorbed lines. All radiative transitions, with the exception of resonance transitions, participate in cascade population of the lowest resonance levels, making it possible to determine the resulting direct excitation cross sections of the 5p 2 P/sub 1/2/ and 5p 2 P/sub 3/2/ levels from the ground state of the silver atom. The part played by cascade population of the resonance levels is not large and is 2 P/sub 3/2/ level, and 10% for the 5p 2 P/sub 1/2/ level, of the excitation cross sections of the corresponding resonance transitions

  10. Electron-impact ionization cross section of rubidium

    International Nuclear Information System (INIS)

    Kim, Y.; Migdalek, J.; Siegel, W.; Bieron, J.

    1998-01-01

    A theoretical model for electron-impact ionization cross section has been applied to Rb and the theoretical cross section (from the threshold to 1 keV in incident energy) is in good agreement with the recent experimental data obtained using Rb atoms trapped in a magneto-optical trap. The theoretical model, called the binary-encounter endash dipole (BED) model, combines a modified Mott cross section with the high-energy behavior of Born cross sections. To obtain the continuum dipole oscillator strength df/dE of the 5s electron required in the BED model, we used Dirac-Fock continuum wave functions with a core polarization potential that reproduced the known position of the Cooper minimum in the photoionization cross section. For inner-shell ionization, we used a simpler version of df/dE, which retained the hydrogenic shape. The contributions of the 4p→4d, 5s, and 5p autoionizing excitations were estimated using the plane-wave Born approximation. As a by-product, we also present the dipole oscillator strengths for the 5s→np 1/2 and 5s→np 3/2 transitions for high principal quantum numbers n near the ionization threshold obtained from the Dirac-Fock wave functions with the same core polarization potential as that used for the continuum wave functions. copyright 1998 The American Physical Society

  11. On analytical fits for electron impact ionisation cross sections

    International Nuclear Information System (INIS)

    Godunov, A.L.; Ivanov, P.B.

    1999-01-01

    The problem of providing accurate recommended analytical fits for electron impact ionisation cross sections is discussed, and a number of approaches are considered on the sample case of neon and its ions. The previously known fits are being reassessed using complete experimental and theoretical data, with the preference for experiment, to avoid systematic shifts introduced by the present calculation methods. The feasibility of the standard BELI formula is investigated in detail, and a number of other analytical expressions is suggested, approximating single-ionization cross sections in the whole range of energies. The factors influencing the accuracy of the fits and the physical meaning of the parameters obtained are discussed. (orig.)

  12. Electron transport in silicon nanowires having different cross-sections

    Directory of Open Access Journals (Sweden)

    Muscato Orazio

    2016-06-01

    Full Text Available Transport phenomena in silicon nanowires with different cross-section are investigated using an Extended Hydrodynamic model, coupled to the Schrödinger-Poisson system. The model has been formulated by closing the moment system derived from the Boltzmann equation on the basis of the maximum entropy principle of Extended Thermodynamics, obtaining explicit closure relations for the high-order fluxes and the production terms. Scattering of electrons with acoustic and non polar optical phonons have been taken into account. The bulk mobility is evaluated for square and equilateral triangle cross-sections of the wire.

  13. Cross-sectional transmission electron microscopy of semiconductors

    International Nuclear Information System (INIS)

    Sadana, D.K.

    1982-10-01

    A method to prepare cross-sectional (X) semiconductor specimens for transmission electron microscopy (TEM) has been described. The power and utility of XTEM has been demonstrated. It has been shown that accuracy and interpretation of indirect structural-defects profiling techniques, namely, MeV He + channeling and secondary ion mass spectrometry (SIMS) can be greatly enhanced by comparing their results with those obtained by XTEM from the same set of samples

  14. Compilation of electron collision excitation cross sections for neutral argon

    International Nuclear Information System (INIS)

    Blanco, F.

    1993-01-01

    The present work presents a compilation and critical analysis of the available data on electron collision excitation cross sections for neutral Argon levels. This study includes: 1.- A detailed description in intermediate coupling for all the levels belonging the 20 configurations 3p5 ns (n=4to 12), np(n=4to8) and nd(n=3to8)of neutral Argon. 2.- Calculation of the electron collision excitation cross sections in Born and Born-Oppenheimer-Ochkur approximations for all the levels in the 14 configurations 3p5 ns (n=4 to 7), np (n=4 to 7) and nd (n=3 to 8). 3.- comparison and discussion of the compiled data. These are the experimental and theoretical values available from the literature, and those from this work. 4.- Analysis of the regularities and systematic behaviors in order to determine which values can be considered more reliable. It is show that the concept of one electron cross section results quite useful for this purpose. In some cases it has been possible to obtain in this way approximate analytical expressions interpolating the experimental data. 5.- All the experimental and theoretical values studied are graphically presented and compared. 6.- The last part of the work includes a listing of several general purpose programs for Atomic Physics calculations developed for this work. (Author) 35 refs

  15. Compilation of electron collision excitation cross sections for neutro argon

    International Nuclear Information System (INIS)

    Blanco Ramos, F.

    1993-01-01

    The present work presents a compilation and critical analysis of the available data on electron collision excitation cross sections for neutral Argon levels. This study includes: 1.- A detailed description in intermediate coupling for all the levels belonging the 20 configurations 3p''5 ns(n=4 to 12), np(n=4 to 8) and nd(n=3 to 8) of neutral Argon. 2.- Calculation of the electron collision excitation cross sections in Born and Born-Oppenheimer-Ochkur approximations for all the levels in the 14 configurations 3p''5 ns(n=4 to 7), np(n=4 to 7) and nd(n=3 to 8). 3.- Comparison and discussion of the compiled data. These are the experimental and theoretical values available from the literature, and those from this work. 4.- Analysis of the regularities and systematic behaviors in order to determine which values can be considered more reliable. It is show that the concept of one electron cross section results quite useful for this purpose. In some cases it has been possible to obtain in this way approximate analytical expressions interpolating the experimental data. 5.- All the experimental and theoretical values studied are graphically presented and compared. 6.- The last part of the work includes a listing of several general purpose programs for Atomic Physics calculations developed for this work. (Author)

  16. Electron-impact-excitation cross sections of hydrogenlike ions

    International Nuclear Information System (INIS)

    Fisher, V.I.; Ralchenko, Y.V.; Bernshtam, V.A.; Goldgirsh, A.; Maron, Y.; Vainshtein, L.A.; Bray, I.; Golten, H.

    1997-01-01

    Convergent close-coupling (CCC) and Coulomb-Born with exchange and normalization (CBE) methods are used to study electron-impact excitation of hydrogenlike ions. The nl→n ' l ' cross sections demonstrate (i) good agreement between the CCC and CBE results, (ii) a scaling over ion nuclear charge z, (iii) a domination of the dipole (l ' =l±1) contributions in total n→n ' cross sections, and (iv) significant effect of electron exchange in the energy range x n,n ' ). For ions with z>5 the n→n ' cross sections obtained in the CCC and CBE approximations agree with each other to better than 10% for any x. An accuracy of the cross sections scaling over z 4 depends on z: for z=6 endash 18 the scaling is accurate to better than 10% (quantitative analysis is done for n ' 4 scaling more significantly (at x about unity). The n→n ' cross sections are presented by a formula which fits our CCC and CBE results with an accuracy to better than 10% (for transitions with n ' 5). The new Gaunt factor G(x) suggested for the widely used Van Regemorter formula [Astrophys. J. 136, 906 (1962)] makes this formula accurate to better than 50% in the x>3 range and to better than 20% in the x>100 range. It is shown that the semiempirical formula by Vainshtein, Sobelman, and Yukov provides an accuracy to better than 50% for any incident electron energy. For x<2 this formula is accurate to better than 30%. These accuracy assessments are based on a comparison with our CCC and CBE results. copyright 1997 The American Physical Society

  17. Higher energy dissociative electron attachment cross sections in sulfur dioxide

    International Nuclear Information System (INIS)

    Kurepa, M.; Pejcev, V.; Cadez, I.

    2000-01-01

    Experimental results of total electron attachment cross sections are presented with, for comparison, two additional sets of data, those of Orient and Srivastava and of Spyrou et al.. Both were normalized to present values of the first attachment peak at 4,6 eV, in order to show more clearly differences in cross section curve shapes. In fact, data of Orient and Srivastava are larger that the present ones for a factor of 2,82; while those of Spyrou et al. are higher only for 3,70 %. Both these sets of data, as well as those by Cadez et al., cover an incident electron energy range 3,40 - 9,40 eV. Electron attachment processes at energies higher that 9,40 eV have been in fact detected and measured in the same set of experiments that led to former publication of lower energy attachment processes by Cadez et al.. At that time in none of experiments, that could distinguished ionic species formed in dissociation attachment processes, was a sign of ions at incident electron energies exceeding 9,40 eV. That caused our ignorance toward processes detected and measured at higher incident electron energies, mainly since they were at least one order of magnitude lower that the two peaks at 4,6 eV and 7,3 eV, respectively. Without additional experiments, that include mass analysis of ionic species formed in dissociative electron attachment processes, it is not possible to give any sound explanation to causes of peaks at energies higher that 8,0 eV

  18. Electron impact ionisation cross sections of iron oxides

    Science.gov (United States)

    Huber, Stefan E.; Mauracher, Andreas; Sukuba, Ivan; Urban, Jan; Maihom, Thana; Probst, Michael

    2017-12-01

    We report electron impact ionisation cross sections (EICSs) of iron oxide molecules, FexOx and FexOx+1 with x = 1, 2, 3, from the ionisation threshold to 10 keV, obtained with the Deutsch-Märk (DM) and binary-encounter-Bethe (BEB) methods. The maxima of the EICSs range from 3.10 to 9 . 96 × 10-16 cm2 located at 59-72 eV and 5.06 to 14.32 × 10-16 cm2 located at 85-108 eV for the DM and BEB approaches, respectively. The orbital and kinetic energies required for the BEB method are obtained by employing effective core potentials for the inner core electrons in the quantum chemical calculations. The BEB cross sections are 1.4-1.7 times larger than the DM cross sections which can be related to the decreasing population of the Fe 4s orbitals upon addition of oxygen atoms, together with the different methodological foundations of the two methods. Both the DM and BEB cross sections can be fitted excellently to a simple analytical expression used in modelling and simulation codes employed in the framework of nuclear fusion research. Supplementary material in the form of one pdf file available from the Journal web page at http://https://doi.org/10.1140/epjd/e2017-80308-2.

  19. Generalized Fokker-Planck theory for electron and photon transport in biological tissues: application to radiotherapy.

    Science.gov (United States)

    Olbrant, Edgar; Frank, Martin

    2010-12-01

    In this paper, we study a deterministic method for particle transport in biological tissues. The method is specifically developed for dose calculations in cancer therapy and for radiological imaging. Generalized Fokker-Planck (GFP) theory [Leakeas and Larsen, Nucl. Sci. Eng. 137 (2001), pp. 236-250] has been developed to improve the Fokker-Planck (FP) equation in cases where scattering is forward-peaked and where there is a sufficient amount of large-angle scattering. We compare grid-based numerical solutions to FP and GFP in realistic medical applications. First, electron dose calculations in heterogeneous parts of the human body are performed. Therefore, accurate electron scattering cross sections are included and their incorporation into our model is extensively described. Second, we solve GFP approximations of the radiative transport equation to investigate reflectance and transmittance of light in biological tissues. All results are compared with either Monte Carlo or discrete-ordinates transport solutions.

  20. Integral elastic, electronic-state, ionization, and total cross sections for electron scattering with furfural

    Science.gov (United States)

    Jones, D. B.; da Costa, R. F.; Varella, M. T. do N.; Bettega, M. H. F.; Lima, M. A. P.; Blanco, F.; García, G.; Brunger, M. J.

    2016-04-01

    We report absolute experimental integral cross sections (ICSs) for electron impact excitation of bands of electronic-states in furfural, for incident electron energies in the range 20-250 eV. Wherever possible, those results are compared to corresponding excitation cross sections in the structurally similar species furan, as previously reported by da Costa et al. [Phys. Rev. A 85, 062706 (2012)] and Regeta and Allan [Phys. Rev. A 91, 012707 (2015)]. Generally, very good agreement is found. In addition, ICSs calculated with our independent atom model (IAM) with screening corrected additivity rule (SCAR) formalism, extended to account for interference (I) terms that arise due to the multi-centre nature of the scattering problem, are also reported. The sum of those ICSs gives the IAM-SCAR+I total cross section for electron-furfural scattering. Where possible, those calculated IAM-SCAR+I ICS results are compared against corresponding results from the present measurements with an acceptable level of accord being obtained. Similarly, but only for the band I and band II excited electronic states, we also present results from our Schwinger multichannel method with pseudopotentials calculations. Those results are found to be in good qualitative accord with the present experimental ICSs. Finally, with a view to assembling a complete cross section data base for furfural, some binary-encounter-Bethe-level total ionization cross sections for this collision system are presented.

  1. Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.

    Science.gov (United States)

    Nishiyama, Hidetoshi; Suga, Mitsuo; Ogura, Toshihiko; Maruyama, Yuusuke; Koizumi, Mitsuru; Mio, Kazuhiro; Kitamura, Shinichi; Sato, Chikara

    2010-03-01

    Direct observation of subcellular structures and their characterization is essential for understanding their physiological functions. To observe them in open environment, we have developed an inverted scanning electron microscope with a detachable, open-culture dish, capable of 8 nm resolution, and combined with a fluorescence microscope quasi-simultaneously observing the same area from the top. For scanning electron microscopy from the bottom, a silicon nitride film window in the base of the dish maintains a vacuum between electron gun and open sample dish while allowing electrons to pass through. Electrons are backscattered from the sample and captured by a detector under the dish. Cells cultured on the open dish can be externally manipulated under optical microscopy, fixed, and observed using scanning electron microscopy. Once fine structures have been revealed by scanning electron microscopy, their component proteins may be identified by comparison with separately prepared fluorescence-labeled optical microscopic images of the candidate proteins, with their heavy-metal-labeled or stained ASEM images. Furthermore, cell nuclei in a tissue block stained with platinum-blue were successfully observed without thin-sectioning, which suggests the applicability of this inverted scanning electron microscope to cancer diagnosis. This microscope visualizes mesoscopic-scale structures, and is also applicable to non-bioscience fields including polymer chemistry. (c) 2010 Elsevier Inc. All rights reserved.

  2. Electron Microscopy Observation of Biomineralization within Wood Tissues of Kurogaki

    Directory of Open Access Journals (Sweden)

    Kazue Tazaki

    2017-07-01

    Full Text Available Interactions between minerals and microorganisms play a crucial role in living wood tissues. However, living wood tissues have never been studied in the field. Fortunately, we found several kurogaki (black persimmon; Diospyros kaki trees at Tawara in Kanazawa, Ishikawa, Japan. Here, we report the characterization of kurogaki based on scanning electron microscopy equipped with energy-dispersive spectroscopy (SEM-EDS and transmission electron microscopy (TEM, associated with inductively coupled plasma-mass spectrometry (ICP-MS analyses, X-ray fluorescence analyses (XRF and X-ray powder diffraction (XRD analyses. This study aims to illustrate the ability of various microorganisms associated with biominerals within wood tissues of kurogaki, as shown by SEM-EDS elemental content maps and TEM images. Kurogaki grows very slowly and has extremely hard wood, known for its striking black and beige coloration, referred to as a “peacock pattern”. However, the scientific data for kurogaki are very limited. The black “peacock pattern” of the wood mainly comprises cellulose and high levels of crystal cristobalite. As per the XRD results, the black taproot contains mineralized 7 Å clays (kaolinite, cellulose, apatite and cristobalite associated with many microorganisms. The chemical compositions of the black and beige portions of the black persimmon tree were obtained by ICP-MS analyses. Particular elements such as abundant Ca, Mg, K, P, Mn, Ba, S, Cl, Fe, Na, and Al were concentrated in the black region, associated with Pb and Sr elements. SEM-EDS semi-qualitative analyses of kurogaki indicated an abundance of P and Ca in microorganisms in the black region, associated with Pb, Sr, S, Mn, and Mg elements. On the other hand, XRF and XRD mineralogical data showed that fresh andesite, weathered andesite, and the soils around the roots of kurogaki correlate with biomineralization of the black region in kurogaki roots, showing clay minerals (kaolinite and

  3. Total cross sections for electron scattering with halocarbon molecules

    Energy Technology Data Exchange (ETDEWEB)

    Naghma, Rahla; Gupta, Dhanoj; Antony, Bobby, E-mail: bka.ism@gmail.com

    2014-03-01

    Highlights: • A quantum mechanical model to find elastic, inelastic and total CS by e{sup −} impact. • Spherical complex optical potential formalism is used to find total CS. • Result shows consistency and good agreement with previous data wherever available. • Maiden attempt to find CS for CH{sub 2}Br{sub 2}, CHBr{sub 3}, CBr{sub 4} and C{sub n}H{sub 2n+1}Cl (n = 2–4) molecules. • Interesting correlation observed between total CS and polarizability of the molecule. - Abstract: A theoretical study on electron collision with chlorinated methanes: CH{sub 2}Cl{sub 2} and CHCl{sub 3}, brominated methanes: CH{sub 2}Br{sub 2}, CHBr{sub 3} and CBr{sub 4} and some mono chloroalkanes: C{sub n}H{sub 2n+1}Cl (n = 2–4) molecules in gaseous ground state is undertaken to report elastic, inelastic and total cross sections in the 20–5000 eV energy range. The target molecule is represented as a sum of various scattering centres, which are assumed to scatter electrons independently. The spherical complex optical potential (SCOP) is formulated to represent the interaction dynamics between the electron and the constituent scattering centres. Using SCOP, the quantum mechanical scattering problem is solved through partial wave analysis. The results obtained for CH{sub 2}Cl{sub 2} and CHCl{sub 3} are compared with the available experimental and theoretical values. The elastic cross section for CBr{sub 4} shows satisfactory agreement with the previous available data. The cross sections for CH{sub 2}Br{sub 2}, CHBr{sub 3}, and C{sub n}H{sub 2n+1}Cl (n = 2–4) molecules presented in this work are reported for the first time.

  4. Two-electron photoionization cross sections at high energies

    International Nuclear Information System (INIS)

    Amusia, M.Ya.; Krivec, R.; Mandelzweig, V.B.

    2003-01-01

    Double and single electron photoionization cross sections and their ratios at high and ultra-relativistic energies are calculated for H - , He and helium-like ions in ground and excited states including triplet states. The ratios contain shake-off and quasi-free terms. A high precision non-variational wave function is used. The quasi-free mechanism increases the ratios impressively: for He we get 0.0762 instead of 0.0164 in the non-relativistic case. Ratios are inversely proportional to Z 2 , with a factor increasing from 0.094 in the nonrelativistic to 0.595 in the ultra-relativistic limit. (author)

  5. Integral elastic, electronic-state, ionization, and total cross sections for electron scattering with furfural

    Energy Technology Data Exchange (ETDEWEB)

    Jones, D. B. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia); Costa, R. F. da [Instituto de Física “Gleb Wataghin,” Universidade Estadual de Campinas, Campinas, 13083-859 São Paulo (Brazil); Departamento de Física, Universidade Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo (Brazil); Varella, M. T. do N. [Instituto de Física, Universidade de São Paulo, CP 66318, 05315-970 São Paulo (Brazil); Bettega, M. H. F. [Departamento de Física, Universidade Federal do Paraná, CP 19044, 81531-990 Curitiba, Paraná (Brazil); Lima, M. A. P. [Instituto de Física “Gleb Wataghin,” Universidade Estadual de Campinas, Campinas, 13083-859 São Paulo (Brazil); Blanco, F. [Departamento de Física Atómica, Molecular y Nuclear, Universidad Complutense de Madrid, Madrid E-28040 (Spain); García, G. [Instituto de Física Fundamental, CSIC, Serrano 113-bis, 28006 Madrid (Spain); Brunger, M. J., E-mail: Michael.Brunger@flinders.edu.au [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia); Institute of Mathematical Sciences, University of Malaya, 50603 Kuala Lumpur (Malaysia)

    2016-04-14

    We report absolute experimental integral cross sections (ICSs) for electron impact excitation of bands of electronic-states in furfural, for incident electron energies in the range 20–250 eV. Wherever possible, those results are compared to corresponding excitation cross sections in the structurally similar species furan, as previously reported by da Costa et al. [Phys. Rev. A 85, 062706 (2012)] and Regeta and Allan [Phys. Rev. A 91, 012707 (2015)]. Generally, very good agreement is found. In addition, ICSs calculated with our independent atom model (IAM) with screening corrected additivity rule (SCAR) formalism, extended to account for interference (I) terms that arise due to the multi-centre nature of the scattering problem, are also reported. The sum of those ICSs gives the IAM-SCAR+I total cross section for electron–furfural scattering. Where possible, those calculated IAM-SCAR+I ICS results are compared against corresponding results from the present measurements with an acceptable level of accord being obtained. Similarly, but only for the band I and band II excited electronic states, we also present results from our Schwinger multichannel method with pseudopotentials calculations. Those results are found to be in good qualitative accord with the present experimental ICSs. Finally, with a view to assembling a complete cross section data base for furfural, some binary-encounter-Bethe-level total ionization cross sections for this collision system are presented.

  6. Integral elastic, electronic-state, ionization, and total cross sections for electron scattering with furfural

    International Nuclear Information System (INIS)

    Jones, D. B.; Costa, R. F. da; Varella, M. T. do N.; Bettega, M. H. F.; Lima, M. A. P.; Blanco, F.; García, G.; Brunger, M. J.

    2016-01-01

    We report absolute experimental integral cross sections (ICSs) for electron impact excitation of bands of electronic-states in furfural, for incident electron energies in the range 20–250 eV. Wherever possible, those results are compared to corresponding excitation cross sections in the structurally similar species furan, as previously reported by da Costa et al. [Phys. Rev. A 85, 062706 (2012)] and Regeta and Allan [Phys. Rev. A 91, 012707 (2015)]. Generally, very good agreement is found. In addition, ICSs calculated with our independent atom model (IAM) with screening corrected additivity rule (SCAR) formalism, extended to account for interference (I) terms that arise due to the multi-centre nature of the scattering problem, are also reported. The sum of those ICSs gives the IAM-SCAR+I total cross section for electron–furfural scattering. Where possible, those calculated IAM-SCAR+I ICS results are compared against corresponding results from the present measurements with an acceptable level of accord being obtained. Similarly, but only for the band I and band II excited electronic states, we also present results from our Schwinger multichannel method with pseudopotentials calculations. Those results are found to be in good qualitative accord with the present experimental ICSs. Finally, with a view to assembling a complete cross section data base for furfural, some binary-encounter-Bethe-level total ionization cross sections for this collision system are presented.

  7. Electron ejection cross sections in electron and ion impact ionization: Ab initio and semiempirical calculations

    International Nuclear Information System (INIS)

    Manson, S.T.; Miller, J.H.; Pacific Northwest Lab., Richland, WA)

    1983-01-01

    Ionization cross sections for heavy ions and electrons incident on various atoms and molecules are required in the modeling of the interaction of radiation with matter. For each case, the energy distribution of secondary electrons (the single differential cross section, SDCS) is needed over a broad range of projectile and secondary electron (delta-ray) energies. In many cases the energy and angular distribution of secondary electrons (the double differential cross section, DDCS) is also necessary. Clearly, it would be desirable to have laboratory SDCS and DDCS measurements for all of the cases required. For a variety of reasons, this is not yet possible. Thus, one must turn elsewhere to obtain the needed cross sections. In this paper, we discuss cross sections obtained in two different ways; ab initio theory based on the first Born approximation, and a semi-empirical method based on the Bethe-Born Approximation. In both cases, results on helium will be presented since the largest amount of data is available in this case. Applications of both methods to other target species are given in the references. The accuracy of the methods and plans for the near future are also discussed. 23 references, 6 figures

  8. A dissociative electron attachment cross-section estimator

    International Nuclear Information System (INIS)

    Munro, James J; Harrison, Stephen; Fujimoto, Milton M; Tennyson, Jonathan

    2012-01-01

    Dissociative electron attachment (DEA) is the major process where molecules are destroyed in low-energy plasmas. DEA cross sections are therefore important for a whole variety of applications but are both hard to measure or compute accurately. A method for estimating DEA cross sections based a simple resonance plus survival model is presented. Test results are presented for DEA of molecular oxygen and molecular chlorine, for which experimental measurements are available for comparison, and SiBr and SiBr 2 , for which no previous data is available. The estimator has been implemented as part of Quantemol-N expert system which uses the R-matrix method to predict resonance positions and widths.

  9. Absolute elastic cross sections for electron scattering from SF6

    International Nuclear Information System (INIS)

    Gulley, R.J.; Uhlmann, L.J.; Dedman, C.J.; Buckman, S.J.; Cho, H.; Trantham, K.W.

    2000-01-01

    Full text: Absolute differential cross sections for vibrationally elastic scattering of electrons from sulphur hexafluoride (SF 6 ) have been measured at fixed angles of 60 deg, 90 deg and 120 deg over the energy range of 5 to 15 eV, and also at 11 fixed energies between 2.7 and 75 eV for scattering angles between 10 deg and 180 deg. These measurements employ the magnetic angle-changing technique of Read and Channing in combination with the relative flow technique to obtain absolute elastic scattering cross sections at backward angles (135 deg to 180 deg) for incident energies below 15 eV. The results reveal some substantial differences with several previous determinations and a reasonably good level of agreement with a recent close coupling calculation

  10. Raman spectroscopic analysis of human skin tissue sections ex-vivo: evaluation of the effects of tissue processing and dewaxing

    Science.gov (United States)

    Ali, Syed M.; Bonnier, Franck; Tfayli, Ali; Lambkin, Helen; Flynn, Kathleen; McDonagh, Vincent; Healy, Claragh; Clive Lee, T.; Lyng, Fiona M.; Byrne, Hugh J.

    2013-06-01

    Raman spectroscopy coupled with K-means clustering analysis (KMCA) is employed to elucidate the biochemical structure of human skin tissue sections and the effects of tissue processing. Both hand and thigh sections of human cadavers were analyzed in their unprocessed and formalin-fixed, paraffin-processed (FFPP), and subsequently dewaxed forms. In unprocessed sections, KMCA reveals clear differentiation of the stratum corneum (SC), intermediate underlying epithelium, and dermal layers for sections from both anatomical sites. The SC is seen to be relatively rich in lipidic content; the spectrum of the subjacent layers is strongly influenced by the presence of melanin, while that of the dermis is dominated by the characteristics of collagen. For a given anatomical site, little difference in layer structure and biochemistry is observed between samples from different cadavers. However, the hand and thigh sections are consistently differentiated for all cadavers, largely based on lipidic profiles. In dewaxed FFPP samples, while the SC, intermediate, and dermal layers are clearly differentiated by KMCA of Raman maps of tissue sections, the lipidic contributions to the spectra are significantly reduced, with the result that respective skin layers from different anatomical sites become indistinguishable. While efficient at removing the fixing wax, the tissue processing also efficiently removes the structurally similar lipidic components of the skin layers. In studies of dermatological processes in which lipids play an important role, such as wound healing, dewaxed samples are therefore not appropriate. Removal of the lipids does however accentuate the spectral features of the cellular and protein components, which may be more appropriate for retrospective analysis of disease progression and biochemical analysis using tissue banks.

  11. Calculation of W for low energy electrons in tissue-equivalent gas. [<10 keV

    Energy Technology Data Exchange (ETDEWEB)

    Dayashankar, [Bhabha Atomic Research Centre, Bombay (India). Div. of Radiation Protection

    1977-11-01

    The mean energy expended per ion pair formed (W-value) in the tissue-equivalent gas for incident electrons of energy up to 10 keV has been calculated in the continuous slowing-down approximation. The effect of secondary and tertiary electrons has been considered by utilizing recent measurements of Opal et al., (1971, J. Chem. Phys., 55,4100) on the energy spectra of low-energy secondary electrons and the Mott formula for the spectra of high-energy secondaries. The results, which are provisional in nature due to the limitations on the accuracy of the input cross-section data and the neglect of the discrete nature of energy loss process, are compared with the available measurements.

  12. Electron impact ionisation cross-sections of 2-heptanone

    Science.gov (United States)

    Vacher, J. R.; Blin-Simiand, N.; Jorand, F.; Pasquiers, S.

    2005-01-01

    The electron impact ionisation of 2-heptanone between 13 and 78 eV is studied using mass spectrometry. CH3C(O)CH2CH2CH2CH2CH3+ and fragment ions are produced with a total cross-section of 5 × 10-16 cm2 towards 50 eV. Two ions, identified as CH3CO+ (43 amu) and CH3C(OH)CH2+ (58 amu), contribute to about 60% of the total cross-section for electron energies above the ionisation threshold. The detected ions are identified using ab initio calculations. For E = 14 eV, the ion of 58 amu is the most abundant followed by an ion of 59 amu identified as being CH3C(OH)CH3+; they result from a bond cleavage with one or two H atom rearrangements. For E >= 48 eV, the ion of 43 amu is the most abundant; it results from an [alpha]-cleavage reaction in the molecular ion.

  13. Research on terahertz properties of rat brain tissue sections during dehydration

    Science.gov (United States)

    Cui, Gangqiang; Liang, Jianfeng; Zhao, Hongwei; Zhao, Xianghui; Chang, Chao

    2018-01-01

    Biological tissue sections are always kept in a system purged with dry nitrogen for the measurement of terahertz spectrum. However, the injected nitrogen will cause dehydration of tissue sections, which will affect the accuracy of spectrum measurement. In this paper, terahertz time-domain spectrometer is used to measure the terahertz spectra of rat brain tissue sections during dehydration. The changes of terahertz properties, including terahertz transmittance, refractive index and extinction coefficient during dehydration are also analyzed. The amplitudes of terahertz time-domain spectra increase gradually during the dehydration process. Besides, the terahertz properties show obvious changes during the dehydration process. All the results indicate that the injected dry nitrogen has a significant effect on the terahertz spectra and properties of tissue sections. This study contributes to further research and application of terahertz technology in biomedical field.

  14. Secondary-electron-production cross sections for electron-impact ionization of helium

    International Nuclear Information System (INIS)

    Goruganthu, R.R.; Bonham, R.A.

    1986-01-01

    Measurements of the double-differential cross sections (DDCS) as a function of the ejected energy, angle, and primary energy for electron-impact ionization of helium are reported at incident energies of 200, 500, 1000, and 2000 eV. The ejection angle is varied from 30 0 to 150 0 in steps of 15 0 . The cross sections were obtained by use of a crossed-beam apparatus with an effusive gas source and a pulsed electron beam. Scattered and ejected electrons were energy analyzed by time-of-flight analysis from 2 eV to the primary energy as a function of the ejection angle. The relative measurements were normalized by matching the experimental elastic differential cross sections to absolute measurements at selected angles. Comparisons of the DDCS with available literature values revealed significant differences. At 2000 eV impact energy, first-Born-approximation calculations of the DDCS were found to be in agreement with the present data for ejected energies between 2 and 40 eV. At large angles and lower incident energies the Born calculation results are lower than the present DDCS. The DDCS were fitted with a Legendre-polynomial expansion as a function of ejection angle for fixed ejected and primary energies. The energy distributions of ejected electrons derived from these fits are presented and used to calculate the total ionization cross section

  15. Aspects of Quantitation in Mass Spectrometry Imaging Investigated on Cryo-Sections of Spiked Tissue Homogenates

    DEFF Research Database (Denmark)

    Hansen, Heidi Toft; Janfelt, Christian

    2016-01-01

    for differences in tissue types in, for example, whole-body imaging, a set of tissue homogenates of different tissue types (lung, liver, kidney, heart, and brain) from rabbit was spiked to the same concentration with the drug amitriptyline and imaged in the same experiment using isotope labeled amitriptyline...... for these results range approximately within a factor of 3 (but for other compounds in other tissues could be higher), underscore the importance of preparing the standard curve in the same matrix as the unknown sample whenever possible. In, for example, whole-body imaging where a diversity of tissue types...... are present, this variation across tissue types will therefore add to the overall uncertainty in quantitation. The tissue homogenates were also used in a characterization of various phenomena in quantitative MSI, such as to study how the signal depends of the thickness of the cryo-section, and to assess...

  16. Integral cross sections for electron impact excitation of vibrational and electronic states in phenol

    Energy Technology Data Exchange (ETDEWEB)

    Neves, R. F. C. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Instituto Federal do Sul de Minas Gerais, Campus Poços de Caldas, Minas Gerais (Brazil); Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); Jones, D. B. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Lopes, M. C. A. [Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); Blanco, F. [Departamento de Física Atómica, Molecular y Nuclear, Universidad Complutense de Madrid, 28040 Madrid (Spain); García, G. [Instituto de Física Fundamental, CSIC, Serrano 113-bis, 28006 Madrid (Spain); Ratnavelu, K. [Institute of Mathematical Sciences, University of Malaya, 50603 Kuala Lumpur (Malaysia); Brunger, M. J., E-mail: Michael.Brunger@flinders.edu.au [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Institute of Mathematical Sciences, University of Malaya, 50603 Kuala Lumpur (Malaysia)

    2015-05-21

    We report on measurements of integral cross sections (ICSs) for electron impact excitation of a series of composite vibrational modes and electronic-states in phenol, where the energy range of those experiments was 15–250 eV. There are currently no other results against which we can directly compare those measured data. We also report results from our independent atom model with screened additivity rule correction computations, namely, for the inelastic ICS (all discrete electronic states and neutral dissociation) and the total ionisation ICS. In addition, for the relevant dipole-allowed excited electronic states, we also report f-scaled Born-level and energy-corrected and f-scaled Born-level (BEf-scaled) ICS. Where possible, our measured and calculated ICSs are compared against one another with the general level of accord between them being satisfactory to within the measurement uncertainties.

  17. Electron-impact ionization doubly differential cross sections of helium

    International Nuclear Information System (INIS)

    Bray, Igor; Fursa, Dmitry V; Stelbovics, Andris T

    2003-01-01

    The convergent close-coupling (CCC) method is applied to the calculation of electron-helium ionization doubly differential cross sections (DDCSs) at low to intermediate incident energies. We re-examine the CCC calculations and measurements of Roeder et al (1997 J. Phys. B: At. Mol. Opt. Phys. 30 1309-22) by making allowance for the step-function behaviour of the underlying CCC-calculated amplitudes. As done previously, the experimental DDCS were normalized at energies below 100 eV using the 100 eV CCC calculation to determine analyser properties at several secondary energies. In addition, substantially larger calculations are presented, to check the convergence. The agreement between the experiment and the calculations as a whole is much improved on the situation reported earlier

  18. Confocal microscopy of thick tissue sections: 3D visualizaiton of rat kidney glomeruli

    Science.gov (United States)

    Confocal laser scanning microscopy (CLSM) as a technique capable of generating serial sections of whole-mount tissue and then reassembling the computer-acquired images as a virtual 3-dimentional structure. In many ways CLSM offers an alternative to traditional sectioning approac...

  19. Confocal Microscopy of thick tissue sections: 3D Visualization of rat kidney glomeruli

    Science.gov (United States)

    Confocal laser scanning microscopy (CLSM) as a technique capable of generating serial sections of whole-mount tissue and then reassembling the computer-acquired images as a virtual 3-dimentional structure. In many ways CLSM offers an alternative to traditional sectioning approac...

  20. Nano-analytical electron microscopy reveals fundamental insights into human cardiovascular tissue calcification

    Science.gov (United States)

    Bertazzo, Sergio; Gentleman, Eileen; Cloyd, Kristy L.; Chester, Adrian H.; Yacoub, Magdi H.; Stevens, Molly M.

    2013-06-01

    The accumulation of calcified material in cardiovascular tissue is thought to involve cytochemical, extracellular matrix and systemic signals; however, its precise composition and nanoscale architecture remain largely unexplored. Using nano-analytical electron microscopy techniques, we examined valves, aortae and coronary arteries from patients with and without calcific cardiovascular disease and detected spherical calcium phosphate particles, regardless of the presence of calcific lesions. We also examined lesions after sectioning with a focused ion beam and found that the spherical particles are composed of highly crystalline hydroxyapatite that crystallographically and structurally differs from bone mineral. Taken together, these data suggest that mineralized spherical particles may play a fundamental role in calcific lesion formation. Their ubiquitous presence in varied cardiovascular tissues and from patients with a spectrum of diseases further suggests that lesion formation may follow a common process. Indeed, applying materials science techniques to ectopic and orthotopic calcification has great potential to lend critical insights into pathophysiological processes underlying calcific cardiovascular disease.

  1. Empirical Fit to Inelastic Electron-Deuteron and Electron-Neutron Resonance Region Transverse Cross Sections

    International Nuclear Information System (INIS)

    Peter Bosted; M. E. Christy

    2007-01-01

    An empirical fit is described to measurements of inclusive inelastic electron-deuteron cross sections in the kinematic range of four-momentum transfer 0 (le) Q 2 2 and final state invariant mass 1.2 p of longitudinal to transverse cross sections for the proton, and the assumption R p =R n . The underlying fit parameters describe the average cross section for proton and neutron, with a plane-wave impulse approximation (PWIA) used to fit to the deuteron data. Pseudo-data from MAID 2007 were used to constrain the average nucleon cross sections for W<1.2 GeV. The mean deviation of data from the fit is 3%, with less than 5% of the data points deviating from the fit by more than 10%

  2. Inversion of the total cross sections for electron-molecule and electron-atom scattering

    International Nuclear Information System (INIS)

    Lun, D.R.; Amos, K.; Allen, L.J.

    1994-01-01

    Inverse scattering theory has been applied to construct the interaction potentials from total cross sections as a function of energy for electrons scattered off of atoms and molecules. The underlying potentials are assumed to be real and energy independent and are evaluated using the Eikonal approximation and with real phase shifts determined from the total cross sections. The inversion potentials have been determined using either a high energy limit approximation or by using a fixed energy inversion method at select energies. These procedures have been used to analyse e - - CH 4 , e - - SiH 4 , e - -Kr and e - -Xe scattering data in particular. 14 refs., 1 tabs., 3 figs

  3. Theoretical and experimental differential cross sections for electron impact excitation of the electronic bands of furfural

    Science.gov (United States)

    Jones, D. B.; Neves, R. F. C.; Lopes, M. C. A.; da Costa, R. F.; do N. Varella, M. T.; Bettega, M. H. F.; Lima, M. A. P.; García, G.; Limão-Vieira, P.; Brunger, M. J.

    2016-03-01

    We report results from a joint experimental and theoretical investigation into electron scattering from the important industrial species furfural (C5H4O2). Specifically, differential cross sections (DCSs) have been measured and calculated for the electron-impact excitation of the electronic states of C5H4O2. The measurements were carried out at energies in the range 20-40 eV, and for scattered-electron angles between 10° and 90°. The energy resolution of those experiments was typically ˜80 meV. Corresponding Schwinger multichannel method with pseudo-potential calculations, for energies between 6-50 eV and with and without Born-closure, were also performed for a sub-set of the excited electronic-states that were accessed in the measurements. Those calculations were undertaken at the static exchange plus polarisation-level using a minimum orbital basis for single configuration interaction (MOB-SCI) approach. Agreement between the measured and calculated DCSs was qualitatively quite good, although to obtain quantitative accord, the theory would need to incorporate even more channels into the MOB-SCI. The role of multichannel coupling on the computed electronic-state DCSs is also explored in some detail.

  4. Theoretical and experimental differential cross sections for electron impact excitation of the electronic bands of furfural

    International Nuclear Information System (INIS)

    Jones, D. B.; Neves, R. F. C.; Lopes, M. C. A.; Costa, R. F. da; Varella, M. T. do N; Bettega, M. H. F.; Lima, M. A. P.; García, G.

    2016-01-01

    We report results from a joint experimental and theoretical investigation into electron scattering from the important industrial species furfural (C 5 H 4 O 2 ). Specifically, differential cross sections (DCSs) have been measured and calculated for the electron-impact excitation of the electronic states of C 5 H 4 O 2 . The measurements were carried out at energies in the range 20–40 eV, and for scattered-electron angles between 10° and 90°. The energy resolution of those experiments was typically ∼80 meV. Corresponding Schwinger multichannel method with pseudo-potential calculations, for energies between 6–50 eV and with and without Born-closure, were also performed for a sub-set of the excited electronic-states that were accessed in the measurements. Those calculations were undertaken at the static exchange plus polarisation-level using a minimum orbital basis for single configuration interaction (MOB-SCI) approach. Agreement between the measured and calculated DCSs was qualitatively quite good, although to obtain quantitative accord, the theory would need to incorporate even more channels into the MOB-SCI. The role of multichannel coupling on the computed electronic-state DCSs is also explored in some detail.

  5. Theoretical and experimental differential cross sections for electron impact excitation of the electronic bands of furfural

    Energy Technology Data Exchange (ETDEWEB)

    Jones, D. B. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Neves, R. F. C. [Instituto Federal do Sul de Minas Gerais, Câmpus Poços de Caldas, Minas Gerais (Brazil); Departamento de Física, UFJF, Juiz de Fora, Minas Gerais 36036-900 (Brazil); Lopes, M. C. A. [Departamento de Física, UFJF, Juiz de Fora, Minas Gerais 36036-900 (Brazil); Costa, R. F. da [Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, São Paulo 09210-580 (Brazil); Instituto de Física “Gleb Wataghin,” Universidade Estadual de Campinas, Campinas, São Paulo 13083-859 (Brazil); Varella, M. T. do N [Instituto de Física, Universidade de São Paulo, CP 66318, 05315-970 São Paulo (Brazil); Bettega, M. H. F. [Departamento de Física, Universidade Federal do Paraná, CP 19044, Curitiba, Paraná 81531-990 (Brazil); Lima, M. A. P., E-mail: maplima@ifi.unicamp.br [Instituto de Física “Gleb Wataghin,” Universidade Estadual de Campinas, Campinas, São Paulo 13083-859 (Brazil); García, G. [Instituto de Física Fundamental, CSIC, Serrano 113-bis, 28006 Madrid (Spain); and others

    2016-03-28

    We report results from a joint experimental and theoretical investigation into electron scattering from the important industrial species furfural (C{sub 5}H{sub 4}O{sub 2}). Specifically, differential cross sections (DCSs) have been measured and calculated for the electron-impact excitation of the electronic states of C{sub 5}H{sub 4}O{sub 2}. The measurements were carried out at energies in the range 20–40 eV, and for scattered-electron angles between 10° and 90°. The energy resolution of those experiments was typically ∼80 meV. Corresponding Schwinger multichannel method with pseudo-potential calculations, for energies between 6–50 eV and with and without Born-closure, were also performed for a sub-set of the excited electronic-states that were accessed in the measurements. Those calculations were undertaken at the static exchange plus polarisation-level using a minimum orbital basis for single configuration interaction (MOB-SCI) approach. Agreement between the measured and calculated DCSs was qualitatively quite good, although to obtain quantitative accord, the theory would need to incorporate even more channels into the MOB-SCI. The role of multichannel coupling on the computed electronic-state DCSs is also explored in some detail.

  6. ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections.

    Science.gov (United States)

    Bruinen, Anne Lisa; Fisher, Gregory L; Heeren, Ron M A

    2017-01-01

    Unambiguous identification of detected species is essential in complex biomedical samples. To date, there are not many mass spectrometry imaging techniques that can provide both high spatial resolution and identification capabilities. A new and patented imaging tandem mass spectrometer, exploiting the unique characteristics of the nanoTOF II (Physical Electronics, USA) TOF-SIMS TRIFT instrument, was developed to address this.Tandem mass spectrometry is based on the selection of precursor ions from the full secondary ion spectrum (MS 1 ), followed by energetic activation and fragmentation, and collection of the fragment ions to obtain a tandem MS spectrum (MS 2 ). The PHI NanoTOF II mass spectrometer is equipped with a high-energy collision induced dissociation (CID) fragmentation cell as well as a second time-of-flight analyzer developed for simultaneous ToF-SIMS and tandem MS imaging experiments.We describe here the results of a ToF-SIMS imaging experiment on a thin tissue section of an infected zebrafish as a model organism for tuberculosis. The focus is on the obtained ion distribution plot of a fatty acid as well as its identification by tandem mass spectrometry.

  7. Authors' perceptions of electronic publishing: two cross sectional surveys.

    Science.gov (United States)

    Schroter, Sara; Barratt, Helen; Smith, Jane

    2004-06-05

    To evaluate how acceptable authors find the BMJ's current practice of publishing short versions of research articles in the paper journal and a longer version on the web and to determine authors' attitudes towards publishing only abstracts in the paper journal and publishing unedited versions on bmj.com once papers have been accepted for publication. Two cross sectional surveys. General medical journal. Survey 1: corresponding authors of a consecutive sample of published BMJ research articles that had undergone the ELPS (electronic long, paper short) process. Survey 2: corresponding authors of consecutive research articles submitted to BMJ. Response rates were 90% (104/115) in survey 1 and 75% (213/283) in survey 2. ELPS is largely acceptable to BMJ authors, but there is some concern that electronic information is not permanent and uncertainty about how versions are referenced. While authors who had experienced ELPS reported some problems with editors shortening papers, most were able to rectify these. Overall, 70% thought that the BMJ should continue to use ELPS; 49% thought that publishing just the abstract in the printed journal with the full version only on bmj.com was unacceptable; and 23% thought it unacceptable to post unedited versions on bmj.com once a paper had been accepted for publication. It is acceptable to authors to publish short versions of research articles in the printed version of a general medical journal with longer versions on the website. Authors dislike the idea of publishing only abstracts in the printed journal but are in favour of posting accepted articles on the website ahead of the printed version.

  8. Empirical fit to inelastic electron-deuteron and electron-neutron resonance region transverse cross sections

    International Nuclear Information System (INIS)

    Bosted, P. E.; Christy, M. E.

    2008-01-01

    An empirical fit is described to measurements of inclusive inelastic electron-deuteron cross sections in the kinematic range of four-momentum transfer 0≤Q 2 2 and final state invariant mass 1.1 p of longitudinal to transverse cross sections for the proton, and the assumption R p =R n . The underlying fit parameters describe the average cross section for a free proton and a free neutron, with a plane-wave impulse approximation used to fit to the deuteron data. Additional fit parameters are used to fill in the dip between the quasi-elastic peak and the Δ(1232) resonance. The mean deviation of data from the fit is 3%, with less than 4% of the data points deviating from the fit by more than 10%

  9. A reliable Differentiation of Mucor from Aspergillus in Tissue Sections with Ultraviolet Illumination

    OpenAIRE

    Senba, Masachika; Toda, Takayoshi; Toda, Yumiko; Hokama, Seitetsu

    1989-01-01

    In tissue, hyphae of mucor are characteristically broad and infrequently septate. However, it may be difficult to distinguish mucor from aspergillus in tissue sections occasionally, because sometimes aspergillus septa are not detected with hematoxylin-eosin (HE), periodic acid Schiff (PAS ), and Grocott's methenamine silver (GMS). In a case, aspergillus septa can be seen under ultraviolet light. Specifically, structures of these septum were clear cut differences in the histological finding be...

  10. Differential cross sections for electron impact excitation of the electronic bands of phenol

    Energy Technology Data Exchange (ETDEWEB)

    Neves, R. F. C. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Instituto Federal do Sul de Minas Gerais, Campus Poços de Caldas, Minas Gerais (Brazil); Departamento de Física, UFJF, Juiz de Fora, Minas Gerais (Brazil); Jones, D. B. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Lopes, M. C. A.; Nixon, K. L. [Departamento de Física, UFJF, Juiz de Fora, Minas Gerais (Brazil); Silva, G. B. da [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso (Brazil); Duque, H. V. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide SA 5001 (Australia); Departamento de Física, UFJF, Juiz de Fora, Minas Gerais (Brazil); Oliveira, E. M. de; Lima, M. A. P. [Instituto de Física “Gleb Wataghin,” Universidade Estadual de Campinas, 13083-859 Campinas, São Paulo (Brazil); Costa, R. F. da [Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 09210-580 Santo André, São Paulo (Brazil); Varella, M. T. do N. [Instituto de Física, Universidade de São Paulo, CP 66318, 05315-970 São Paulo (Brazil); Bettega, M. H. F. [Departamento de Física, Universidade Federal do Paraná, CP 19044, 81531-990 Curitiba, Paraná (Brazil); and others

    2015-03-14

    We report results from a joint theoretical and experimental investigation into electron scattering from the important organic species phenol (C{sub 6}H{sub 5}OH). Specifically, differential cross sections (DCSs) have been measured and calculated for the electron-impact excitation of the electronic states of C{sub 6}H{sub 5}OH. The measurements were carried out at energies in the range 15–40 eV, and for scattered-electron angles between 10{sup ∘} and 90{sup ∘}. The energy resolution of those experiments was typically ∼80 meV. Corresponding Schwinger multichannel method with pseudo-potentials calculations, with and without Born-closure, were also performed for a sub-set of the excited electronic-states that were accessed in the measurements. Those calculations were conducted at the static exchange plus polarisation (SEP)-level using a minimum orbital basis for single configuration interaction (MOBSCI) approach. Agreement between the measured and calculated DCSs was typically fair, although to obtain quantitative accord, the theory would need to incorporate even more channels into the MOBSCI.

  11. Jet cross sections in electron-positron annihilation

    International Nuclear Information System (INIS)

    Sharpe, S.R.

    1983-08-01

    There are few, if any, detailed tests of QCD at present. I disucss the problems associated with testing QCD and argue that the three jet angular distribution in electron-positron annihilation is a good quantity to use because it minimizes the sensitivity to nonperturbative effects and provides a test of the detailed structure of QCD. However, for such a test to be possible, the perturbation expansion must be well behaved. I present a calculation of the perturbative corrections using two different generalizations of the Sterman-Weinberg two-jet cross section. I argue that because of the uncertainties in our understanding of hadronization it is necessary that the corrections to both these cross sections be small. In presenting the results I use the recent proof of Mukti and Sterman that all the logarithms can be resummed. I find that at Z-factory energies there is a substantial region of parameters defining the jets for which the correction is small, but that there is no such region at PEP/PETRA energies. This problem at PEP/PETRA energies is made worse by the results of a study of the effects of hadronization. Using a simple model I find very significant effects at PEP/PETRA energies that would make a test difficult. These effects do not, however, present problems at Z-factory energies. I conclude that, even if there are further theoretical advances, testing QCD at PEP/PETRA energies using the three-jet angular distribution will be very difficult. However, this distribution can be used to test QCD in a detailed way at Z-factory energies. The corrected results, furthermore, show a systematic difference from the lowest order result that may be measurable at Z-factory energies. If this effect could be measured it would provide a yet more detailed test of QCD, testing, for example, the three gluon coupling. 66 references

  12. Secondary-electron-production cross sections for electron-impact ionization of molecular nitrogen

    International Nuclear Information System (INIS)

    Goruganthu, R.R.; Wilson, W.G.; Bonham, R.A.

    1987-01-01

    Measurements of the double-differential cross section (DDCS), as a function of the ejected energy, angle, and primary energy for electron-impact ionization of molecular nitrogen are reported at incident energies of 200, 500, 1000, and 2000 eV. The ejection angle was varied from 30 0 to 150 0 in steps of 15 0 . The cross sections were obtained by use of a crossed-beam apparatus with an effusive gas source and a pulsed electron beam. Scattered and ejected electrons were energy analyzed by time-of-flight analysis from below 2 eV to the primary energy. The relative measurements were placed on an absolute scale by matching the experimental elastic differential cross sections to absolute measurements at each primary energy. Comparisons of the DDCS with previous reported values revealed significant differences. The DDCS were fitted to a Legendre polynomial expansion as a function of the ejection angle. Platzman plot analysis was carried out on the energy distributions determined from the fit coefficients. The total ionization cross sections at these primary energies were deduced from this plot. An autoionization feature at 2.3 eV was observed for the first time in measurements of this nature and has been assigned as due to a Rydberg state converging to the B 2 Σ/sub u/ + ionic state which decays to the X 2 Σ/sub g/ + ground state of N 2 + . An analysis of the autoionization lines observed in the present work in the range 0.4--2.5 eV is also presented

  13. Backscattered electron SEM imaging of resin sections from plant specimens: observation of histological to subcellular structure and CLEM.

    Science.gov (United States)

    Rizzo, N W; Duncan, K E; Bourett, T M; Howard, R J

    2016-08-01

    We have refined methods for biological specimen preparation and low-voltage backscattered electron imaging in the scanning electron microscope that allow for observation at continuous magnifications of ca. 130-70 000 X, and documentation of tissue and subcellular ultrastructure detail. The technique, based upon early work by Ogura & Hasegawa (1980), affords use of significantly larger sections from fixed and resin-embedded specimens than is possible with transmission electron microscopy while providing similar data. After microtomy, the sections, typically ca. 750 nm thick, were dried onto the surface of glass or silicon wafer and stained with heavy metals-the use of grids avoided. The glass/wafer support was then mounted onto standard scanning electron microscopy sample stubs, carbon-coated and imaged directly at an accelerating voltage of 5 kV, using either a yttrium aluminum garnet or ExB backscattered electron detector. Alternatively, the sections could be viewed first by light microscopy, for example to document signal from a fluorescent protein, and then by scanning electron microscopy to provide correlative light/electron microscope (CLEM) data. These methods provide unobstructed access to ultrastructure in the spatial context of a section ca. 7 × 10 mm in size, significantly larger than the typical 0.2 × 0.3 mm section used for conventional transmission electron microscopy imaging. Application of this approach was especially useful when the biology of interest was rare or difficult to find, e.g. a particular cell type, developmental stage, large organ, the interface between cells of interacting organisms, when contextual information within a large tissue was obligatory, or combinations of these factors. In addition, the methods were easily adapted for immunolocalizations. © 2015 The Author. Journal of Microscopy published by John Wiley & Sons, Ltd on behalf of the Royal Microscopical Society.

  14. Chord-based versus voxel-based methods of electron transport in the skeletal tissues

    International Nuclear Information System (INIS)

    Shah, Amish P.; Jokisch, Derek W.; Rajon, Didier A.; Watchman, Christopher J.; Patton, Phillip W.; Bolch, Wesley E.

    2005-01-01

    Anatomic models needed for internal dose assessment have traditionally been developed using mathematical surface equations to define organ boundaries, shapes, and their positions within the body. Many researchers, however, are now advocating the use of tomographic models created from segmented patient computed tomography (CT) or magnetic resonance (MR) scans. In the skeleton, however, the tissue structures of the bone trabeculae, marrow cavities, and endosteal layer are exceedingly small and of complex shape, and thus do not lend themselves easily to either stylistic representations or in-vivo CT imaging. Historically, the problem of modeling the skeletal tissues has been addressed through the development of chord-based methods of radiation particle transport, as given by studies at the University of Leeds (Leeds, UK) using a 44-year male subject. We have proposed an alternative approach to skeletal dosimetry in which excised sections of marrow-intact cadaver spongiosa are imaged directly via microCT scanning. The cadaver selected for initial investigation of this technique was a 66-year male subject of nominal body mass index (22.7 kg m -2 ). The objectives of the present study were to compare chord-based versus voxel-based methods of skeletal dosimetry using data from the UF 66-year male subject. Good agreement between chord-based and voxel-based transport was noted for marrow irradiation by either bone surface or bone volume sources up to 500-1000 keV (depending upon the skeletal site). In contrast, chord-based models of electron transport yielded consistently lower values of the self-absorbed fraction to marrow tissues than seen under voxel-based transport at energies above 100 keV, a feature directly attributed to the inability of chord-based models to account for nonlinear electron trajectories. Significant differences were also noted in the dosimetry of the endosteal layer (for all source tissues), with chord-based transport predicting a higher fraction of

  15. Cross sections for inelastic scattering of electrons by atoms: selected topics related to electron microscopy

    International Nuclear Information System (INIS)

    Inokuti, M.; Manson, S.T.

    1982-01-01

    We begin with a resume of the Bethe theory, which provides a general framework for discussing the inelastic scattering of fast electrons and leads to powerful criteria for judging the reliability of cross-section data. The central notion of the theory is the generalized oscillator strength as a function of both the energy transfer and the momentum transfer, and is the only non-trivial factor in the inelastic-scattering cross section. Although the Bethe theory was initially conceived for free atoms, its basic ideas apply to solids, with suitable generalizations; in this respect, the notion of the dielectric response function is the most fundamental. Topics selected for discussion include the generalized oscillator strengths for the K-shell and L-shell ionization for all atoms with Z less than or equal to 30, evaluated by use of the Hartree-Slater potential. As a function of the energy transfer, the generalized oscillator strength most often shows a non-monotonic structure near the K-shell and L-shell thresholds, which has been interpreted as manifestations of electron-wave propagation through atomic fields. For molecules and solids, there are additional structures due to the scattering of ejected electrons by the fields of other atoms

  16. Cross sections for electron and photon processes required by electron-transport calculations

    International Nuclear Information System (INIS)

    Peek, J.M.

    1979-11-01

    Electron-transport calculations rely on a large collection of electron-atom and photon-atom cross-section data to represent the response characteristics of the target medium. These basic atomic-physics quantities, and certain qualities derived from them that are now commonly in use, are critically reviewed. Publications appearing after 1978 are not given consideration. Processes involving electron or photon energies less than 1 keV are ignored, while an attempt is made to exhaustively cover the remaining independent parameters and target possibilities. Cases for which data improvements can be made from existing information are identified. Ranges of parameters for which state-of-the-art data are not available are sought out, and recommendations for explicit measurements and/or calculations with presently available tools are presented. An attempt is made to identify the maturity of the atomic-physics data and to predict the possibilities for rapid changes in the quality of the data. Finally, weaknesses in the state-of-the-art atomic-physics data and in the conceptual usage of these data in the context of electron-transport theory are discussed. Brief attempts are made to weight the various aspects of these questions and to suggest possible remedies

  17. Aspects of Quantitation in Mass Spectrometry Imaging Investigated on Cryo-Sections of Spiked Tissue Homogenates.

    Science.gov (United States)

    Hansen, Heidi Toft; Janfelt, Christian

    2016-12-06

    Internal standards have been introduced in quantitative mass spectrometry imaging in order to compensate for differences in intensities throughout an image caused by, for example, difference in ion suppression or analyte extraction efficiency. To test how well the internal standards compensate for differences in tissue types in, for example, whole-body imaging, a set of tissue homogenates of different tissue types (lung, liver, kidney, heart, and brain) from rabbit was spiked to the same concentration with the drug amitriptyline and imaged in the same experiment using isotope labeled amitriptyline as internal standard. The results showed, even after correction with internal standard, significantly lower intensities from brain and to some extent also lung tissue, differences which may be ascribed to binding of the drug to proteins or lipids as known from traditional bioanalysis. The differences, which for these results range approximately within a factor of 3 (but for other compounds in other tissues could be higher), underscore the importance of preparing the standard curve in the same matrix as the unknown sample whenever possible. In, for example, whole-body imaging where a diversity of tissue types are present, this variation across tissue types will therefore add to the overall uncertainty in quantitation. The tissue homogenates were also used in a characterization of various phenomena in quantitative MSI, such as to study how the signal depends of the thickness of the cryo-section, and to assess the accuracy of calibration by droplet deposition. For experiments on liver tissue, calibration by spiked tissue homogenates and droplet deposition was found to provide highly similar results and in both cases linearity with R 2 values of 0.99. In the process, a new method was developed for preparation of standard curves of spiked tissue homogenates, based on the drilling of holes in a block of frozen liver homogenate, providing easy cryo-slicing and good quantitative

  18. Electron capture cross sections by O+ from atomic He

    International Nuclear Information System (INIS)

    Joseph, Dwayne C; Saha, Bidhan C

    2009-01-01

    The adiabatic representation is used in both the quantal and semi classical molecular orbital close coupling methods (MOCC) to evaluate charge exchange cross sections. Our results show good agreement with experimental cross sections

  19. Electron capture cross sections by O+ from atomic He

    Science.gov (United States)

    Joseph, Dwayne C.; Saha, Bidhan C.

    2009-11-01

    The adiabatic representation is used in both the quantal and semi classical molecular orbital close coupling methods (MOCC) to evaluate charge exchange cross sections. Our results show good agreement with experimental cross sections

  20. Electron molecule cross sections relevant to negative ion sources and divertor plasmas

    International Nuclear Information System (INIS)

    Celiberto, R.; Capitelli, M.; Lamanna, U.T.; Janev, R.K.

    1996-01-01

    Electron-molecule cross sections for electronic transitions in H 2 and D 2 molecules vibrationally excited are presented, and a scaling law for the vibrational cross sections is discussed for the X 1 summation + g →B 1 summation + u electronic transition. copyright 1996 American Institute of Physics

  1. 3D prostate histology image reconstruction: Quantifying the impact of tissue deformation and histology section location

    Directory of Open Access Journals (Sweden)

    Eli Gibson

    2013-01-01

    Full Text Available Background: Guidelines for localizing prostate cancer on imaging are ideally informed by registered post-prostatectomy histology. 3D histology reconstruction methods can support this by reintroducing 3D spatial information lost during histology processing. The need to register small, high-grade foci drives a need for high accuracy. Accurate 3D reconstruction method design is impacted by the answers to the following central questions of this work. (1 How does prostate tissue deform during histology processing? (2 What spatial misalignment of the tissue sections is induced by microtome cutting? (3 How does the choice of reconstruction model affect histology reconstruction accuracy? Materials and Methods: Histology, paraffin block face and magnetic resonance images were acquired for 18 whole mid-gland tissue slices from six prostates. 7-15 homologous landmarks were identified on each image. Tissue deformation due to histology processing was characterized using the target registration error (TRE after landmark-based registration under four deformation models (rigid, similarity, affine and thin-plate-spline [TPS]. The misalignment of histology sections from the front faces of tissue slices was quantified using manually identified landmarks. The impact of reconstruction models on the TRE after landmark-based reconstruction was measured under eight reconstruction models comprising one of four deformation models with and without constraining histology images to the tissue slice front faces. Results: Isotropic scaling improved the mean TRE by 0.8-1.0 mm (all results reported as 95% confidence intervals, while skew or TPS deformation improved the mean TRE by <0.1 mm. The mean misalignment was 1.1-1.9΀ (angle and 0.9-1.3 mm (depth. Using isotropic scaling, the front face constraint raised the mean TRE by 0.6-0.8 mm. Conclusions: For sub-millimeter accuracy, 3D reconstruction models should not constrain histology images to the tissue slice front faces and

  2. The importance of fast neutron scattering cross sections for neutron dosimetry in soft tissues

    International Nuclear Information System (INIS)

    Jahr, R.; Brede, H.J.

    1979-05-01

    Tissue equivalent plastic materials are used for the construction of accurate neutron dosemeters. As compared to real tissue, in materials most of the oxygen content is replaced by carbon. In order to determine the dose to human tissue a kerma correction factor has to be used. It is shown that the uncertainty (corresponding to 1 delta) of the correction factor at E = 14.5 MeV amounts to at least 5.2%. An important contribution to the uncertainties results from the lack of experimental data of the 12 C(n, n' 3α), 16 O(n,n'p) and 16 O(n,n'α)-cross-sections. These data are to be calculated by subtracting all other cross sections from the total cross section of ( 16 O + n) and ( 12 C + n). It is shown that the uncertainties of the kerma correction factor can be considerably reduced by an accurate measurement of the scattering cross sections of carbon and oxygen. (orig.) [de

  3. In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections.

    Science.gov (United States)

    Ellison, Mitchell A; McMahon, Michael B; Bonde, Morris R; Palmer, Cristi L; Luster, Douglas G

    2016-01-01

    Rust fungi are obligate pathogens with multiple life stages often including different spore types and multiple plant hosts. While individual rust pathogens are often associated with specific plants, a wide range of plant species are infected with rust fungi. To study the interactions between these important pathogenic fungi and their host plants, one must be able to differentiate fungal tissue from plant tissue. This can be accomplished using the In situ hybridization (ISH) protocol described here. To validate reproducibility using the ISH protocol, samples of Chrysanthemum × morifolium infected with Puccinia horiana, Gladiolus × hortulanus infected with Uromyces transversalis and Glycine max infected with Phakopsora pachyrhizi were tested alongside uninfected leaf tissue samples. The results of these tests show that this technique clearly distinguishes between rust pathogens and their respective host plant tissues. This ISH protocol is applicable to rust fungi and potentially other plant pathogenic fungi as well. It has been shown here that this protocol can be applied to pathogens from different genera of rust fungi with no background staining of plant tissue. We encourage the use of this protocol for the study of plant pathogenic fungi in paraffin embedded sections of host plant tissue.

  4. Assessment of doses caused by electrons in thin layers of tissue-equivalent materials, using MCNP.

    Science.gov (United States)

    Heide, Bernd

    2013-10-01

    Absorbed doses caused by electron irradiation were calculated with Monte Carlo N-Particle transport code (MCNP) for thin layers of tissue-equivalent materials. The layers were so thin that the calculation of energy deposition was on the border of the scope of MCNP. Therefore, in this article application of three different methods of calculation of energy deposition is discussed. This was done by means of two scenarios: in the first one, electrons were emitted from the centre of a sphere of water and also recorded in that sphere; and in the second, an irradiation with the PTB Secondary Standard BSS2 was modelled, where electrons were emitted from an (90)Sr/(90)Y area source and recorded inside a cuboid phantom made of tissue-equivalent material. The speed and accuracy of the different methods were of interest. While a significant difference in accuracy was visible for one method in the first scenario, the difference in accuracy of the three methods was insignificant for the second one. Considerable differences in speed were found for both scenarios. In order to demonstrate the need for calculating the dose in thin small zones, a third scenario was constructed and simulated as well. The third scenario was nearly equal to the second one, but a pike of lead was assumed to be inside the phantom in addition. A dose enhancement (caused by the pike of lead) of ∼113 % was recorded for a thin hollow cylinder at a depth of 0.007 cm, which the basal-skin layer is referred to in particular. Dose enhancements between 68 and 88 % were found for a slab with a radius of 0.09 cm for all depths. All dose enhancements were hardly noticeable for a slab with a cross-sectional area of 1 cm(2), which is usually applied to operational radiation protection.

  5. Automatic registration of multi-modal microscopy images for integrative analysis of prostate tissue sections

    International Nuclear Information System (INIS)

    Lippolis, Giuseppe; Edsjö, Anders; Helczynski, Leszek; Bjartell, Anders; Overgaard, Niels Chr

    2013-01-01

    Prostate cancer is one of the leading causes of cancer related deaths. For diagnosis, predicting the outcome of the disease, and for assessing potential new biomarkers, pathologists and researchers routinely analyze histological samples. Morphological and molecular information may be integrated by aligning microscopic histological images in a multiplex fashion. This process is usually time-consuming and results in intra- and inter-user variability. The aim of this study is to investigate the feasibility of using modern image analysis methods for automated alignment of microscopic images from differently stained adjacent paraffin sections from prostatic tissue specimens. Tissue samples, obtained from biopsy or radical prostatectomy, were sectioned and stained with either hematoxylin & eosin (H&E), immunohistochemistry for p63 and AMACR or Time Resolved Fluorescence (TRF) for androgen receptor (AR). Image pairs were aligned allowing for translation, rotation and scaling. The registration was performed automatically by first detecting landmarks in both images, using the scale invariant image transform (SIFT), followed by the well-known RANSAC protocol for finding point correspondences and finally aligned by Procrustes fit. The Registration results were evaluated using both visual and quantitative criteria as defined in the text. Three experiments were carried out. First, images of consecutive tissue sections stained with H&E and p63/AMACR were successfully aligned in 85 of 88 cases (96.6%). The failures occurred in 3 out of 13 cores with highly aggressive cancer (Gleason score ≥ 8). Second, TRF and H&E image pairs were aligned correctly in 103 out of 106 cases (97%). The third experiment considered the alignment of image pairs with the same staining (H&E) coming from a stack of 4 sections. The success rate for alignment dropped from 93.8% in adjacent sections to 22% for sections furthest away. The proposed method is both reliable and fast and therefore well suited

  6. Beam-Based Alignment of Magnetic Field in the Fermilab Electron Cooler Cooling Section

    International Nuclear Information System (INIS)

    Seletskiy, S. M.; Tupikov, V.

    2006-01-01

    The Fermilab Electron Cooling Project requires low effective anglular spread of electrons in the cooling section. One of the main components of the effective electron angles is an angle of electron beam centroid with respect to antiproton beam. This angle is caused by the poor quality of magnetic field in the 20 m long cooling section solenoid and by the mismatch of the beam centroid to the entrance of the cooling section. This paper focuses on the beam-based procedure of the alignment of the cooling section field and beam centroid matching. The discussed procedure allows to suppress the beam centroid angles below the critical value of 0.1 mrad

  7. Electron transport in furfural: dependence of the electron ranges on the cross sections and the energy loss distribution functions

    Science.gov (United States)

    Ellis-Gibbings, L.; Krupa, K.; Colmenares, R.; Blanco, F.; Muńoz, A.; Mendes, M.; Ferreira da Silva, F.; Limá Vieira, P.; Jones, D. B.; Brunger, M. J.; García, G.

    2016-09-01

    Recent theoretical and experimental studies have provided a complete set of differential and integral electron scattering cross section data from furfural over a broad energy range. The energy loss distribution functions have been determined in this study by averaging electron energy loss spectra for different incident energies and scattering angles. All these data have been used as input parameters for an event by event Monte Carlo simulation procedure to obtain the electron energy deposition patterns and electron ranges in liquid furfural. The dependence of these results on the input cross sections is then analysed to determine the uncertainty of the simulated values.

  8. Immunocytochemistry of formalin-fixed human brain tissues: microwave irradiation of free-floating sections.

    Science.gov (United States)

    Shiurba, R A; Spooner, E T; Ishiguro, K; Takahashi, M; Yoshida, R; Wheelock, T R; Imahori, K; Cataldo, A M; Nixon, R A

    1998-01-01

    Formalin fixation, the chemical process in which formaldehyde binds to cells and tissues, is widely used to preserve human brain specimens from autolytic decomposition. Ultrastructure of cellular and mitochondrial membranes is markedly altered by vesiculation, but this does not interfere with diagnostic evaluation of neurohistology by light microscopy. Serious difficulties are encountered, however, when immunocytochemical staining is attempted. Antigens that are immunoreactive in unfixed frozen sections and protein extracts appear to be concealed or destroyed in formalin-fixed tissues. In dilute aqueous solution, formaldehyde is in equilibrium with methylene glycol and its polymeric hydrates, the balance by far in favor of methylene glyco. Carbonylic formaldehyde is a reactive electrophilic species well known for crosslinking functional groups in tissue proteins, nucleic acids, and polysaccharides. Some of its methylene crosslinks are readily hydrolyzed. Others are stable and irreversible. During immunostaining reactions, intra- and inter-molecular links between macromolecules limit antibody permeation of tissue sections, alter protein secondary structure, and reduce accessibility of antigenic determinants . Accordingly, immunoreactivity is diminished for many antigens. Tissues are rapidly penetrated by methylene glycol, but formaldehyde binding to cellular constituents is relatively slow, increasing progressively until equilibrium is reached. In addition, prolonged storage in formalin may result in acidification of human brain specimens. Low pH favors dissociation of methylene glycol into formaldehyde, further reducing both classical staining and antigen detectability. Various procedures have been devised to counter the antigen masking effects of formaldehyde. Examples include pretreatment of tissue sections with proteases, formic acid, or ultrasound. Recently, heating of mounted sections in ionic salt solution by microwave energy was found to restore many

  9. Optical histology: a method to visualize microvasculature in thick tissue sections of mouse brain.

    Directory of Open Access Journals (Sweden)

    Austin J Moy

    Full Text Available The microvasculature is the network of blood vessels involved in delivering nutrients and gases necessary for tissue survival. Study of the microvasculature often involves immunohistological methods. While useful for visualizing microvasculature at the µm scale in specific regions of interest, immunohistology is not well suited to visualize the global microvascular architecture in an organ. Hence, use of immunohistology precludes visualization of the entire microvasculature of an organ, and thus impedes study of global changes in the microvasculature that occur in concert with changes in tissue due to various disease states. Therefore, there is a critical need for a simple, relatively rapid technique that will facilitate visualization of the microvascular network of an entire tissue.The systemic vasculature of a mouse is stained with the fluorescent lipophilic dye DiI using a method called "vessel painting". The brain, or other organ of interest, is harvested and fixed in 4% paraformaldehyde. The organ is then sliced into 1 mm sections and optically cleared, or made transparent, using FocusClear, a proprietary optical clearing agent. After optical clearing, the DiI-labeled tissue microvasculature is imaged using confocal fluorescence microscopy and adjacent image stacks tiled together to produce a depth-encoded map of the microvasculature in the tissue slice. We demonstrated that the use of optical clearing enhances both the tissue imaging depth and the estimate of the vascular density. Using our "optical histology" technique, we visualized microvasculature in the mouse brain to a depth of 850 µm.Presented here are maps of the microvasculature in 1 mm thick slices of mouse brain. Using combined optical clearing and optical imaging techniques, we devised a methodology to enhance the visualization of the microvasculature in thick tissues. We believe this technique could potentially be used to generate a three-dimensional map of the

  10. Electron probe microanalysis for clinical investigations: Microdrop and soft tissue analysis

    International Nuclear Information System (INIS)

    Ingram, M.J.; Ingram, F.D.

    1984-01-01

    The most important advantage offered by electron probe microanalysis (EPA) for clinical investigations is the ability to analyze smaller volumes of tissue than is possible with conventional techniques. The sample can be a biological soft tissue specimen, which involves subcellular localization, or a picoliter fluid droplet. In either case, the analysis can be nondestructive and permit multiple analyses for a number of elements in a given sample. The most highly developed electron microprobe analytical technique is fluid drop analysis, popularly referred to as microdrop analysis. This method provides the investigator with an analytic capability that has an accuracy of measurement often 1% or better on 20 to 30 picoliter fluid droplets. Electron microprobe techniques have been used for studies of animal hard tissue and for studies that involve insoluble inclusions. However, the development of techniques for studies of labile constituents in animal soft tissue has been much slower. It has been necessary not only to develop appropriate methods of tissue preparation, but also to establish sound techniques for tissue collection. Although there are adequate methods for collection of most types of tissue from laboratory animals, many of these methods are not suitable for human subjects. In order to provide the reader with a better understanding of the capabilities and potential for the application of electron microprobe methodology to problems in clinical medicine, the authors discuss some of their experiences with liquid droplet analysis and quantitative electrolyte distribution measurements in animal soft tissue

  11. Section 508 Electronic Information Accessibility Requirements for Software Development

    Science.gov (United States)

    Ellis, Rebecca

    2014-01-01

    Section 508 Subpart B 1194.21 outlines requirements for operating system and software development in order to create a product that is accessible to users with various disabilities. This portion of Section 508 contains a variety of standards to enable those using assistive technology and with visual, hearing, cognitive and motor difficulties to access all information provided in software. The focus on requirements was limited to the Microsoft Windows® operating system as it is the predominant operating system used at this center. Compliance with this portion of the requirements can be obtained by integrating the requirements into the software development cycle early and by remediating issues in legacy software if possible. There are certain circumstances with software that may arise necessitating an exemption from these requirements, such as design or engineering software using dynamically changing graphics or numbers to convey information. These exceptions can be discussed with the Section 508 Coordinator and another method of accommodation used.

  12. Neuroanatomy from Mesoscopic to Nanoscopic Scales: An Improved Method for the Observation of Semithin Sections by High-Resolution Scanning Electron Microscopy.

    Science.gov (United States)

    Rodríguez, José-Rodrigo; Turégano-López, Marta; DeFelipe, Javier; Merchán-Pérez, Angel

    2018-01-01

    Semithin sections are commonly used to examine large areas of tissue with an optical microscope, in order to locate and trim the regions that will later be studied with the electron microscope. Ideally, the observation of semithin sections would be from mesoscopic to nanoscopic scales directly, instead of using light microscopy and then electron microscopy (EM). Here we propose a method that makes it possible to obtain high-resolution scanning EM images of large areas of the brain in the millimeter to nanometer range. Since our method is compatible with light microscopy, it is also feasible to generate hybrid light and electron microscopic maps. Additionally, the same tissue blocks that have been used to obtain semithin sections can later be used, if necessary, for transmission EM, or for focused ion beam milling and scanning electron microscopy (FIB-SEM).

  13. Direct molecular analysis of whole-body animal tissue sections by MALDI imaging mass spectrometry.

    Science.gov (United States)

    Reyzer, Michelle L; Chaurand, Pierre; Angel, Peggi M; Caprioli, Richard M

    2010-01-01

    The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool for localizing compounds of biological interest with molecular specificity and relatively high resolution. Utilizing imaging mass spectrometry for whole-body animal sections offers considerable analytical advantages compared to traditional methods, such as whole-body autoradiography, but the experiment is not straightforward. This chapter addresses the advantages and unique challenges that the application of imaging mass spectrometry to whole-body animal sections entails, including discussions of sample preparation, matrix application, signal normalization, and image generation. Lipid and protein images obtained from whole-body tissue sections of mouse pups are presented along with detailed protocols for the experiments.

  14. Electron density values of various human tissues: in vitro Compton scatter measurements and calculated ranges

    International Nuclear Information System (INIS)

    Shrimpton, P.C.

    1981-01-01

    Accurate direct measurements of electron density have been performed on specimens from 10 different tissue types of the human body, representing the major organs, using a Compton scatter technique. As a supplement to these experimental values, calculations have been carried out to determine the electron densities expected for these tissue types. The densities observed are in good agreement with the broad ranges deduced from the basic data previously published. The results of both the in vitro sample measurements and the approximate calculations indicate that the electron density of most normal healthy soft tissue can be expected to fall within the fairly restricted range of +- 5% around 3.4 X 10 23 electrons per cm 3 . The obvious exception to this generalisation is the result for lung tissue, which falls considerably below this range owing to the high air content inherent in its construction. In view of such an overall limited variation with little difference between tissues, it would appear that electron density alone is likely to be a rather poor clinical parameter for tissue analysis, with high accuracy and precision being essential in any in vivo Compton measurements for imaging or diagnosis on specific organs. (author)

  15. Electron holography study of the charging effect in microfibrils of sciatic nerve tissues.

    Science.gov (United States)

    Kim, Ki Hyun; Akase, Zentaro; Shindo, Daisuke; Ohno, Nobuhiko; Fujii, Yasuhisa; Terada, Nobuo; Ohno, Shinichi

    2013-08-01

    The charging effects of microfibrils of sciatic nerve tissues due to electron irradiation are investigated using electron holography. The phenomenon that the charging effects are enhanced with an increase of electron intensity is visualized through direct observations of the electric potential distribution around the specimen. The electric potential at the surface of the specimen could be quantitatively evaluated by simulation, which takes into account the reference wave modulation due to the long-range electric field.

  16. Reprint of: Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.

    Science.gov (United States)

    Nishiyama, Hidetoshi; Suga, Mitsuo; Ogura, Toshihiko; Maruyama, Yuusuke; Koizumi, Mitsuru; Mio, Kazuhiro; Kitamura, Shinichi; Sato, Chikara

    2010-11-01

    Direct observation of subcellular structures and their characterization is essential for understanding their physiological functions. To observe them in open environment, we have developed an inverted scanning electron microscope with a detachable, open-culture dish, capable of 8 nm resolution, and combined with a fluorescence microscope quasi-simultaneously observing the same area from the top. For scanning electron microscopy from the bottom, a silicon nitride film window in the base of the dish maintains a vacuum between electron gun and open sample dish while allowing electrons to pass through. Electrons are backscattered from the sample and captured by a detector under the dish. Cells cultured on the open dish can be externally manipulated under optical microscopy, fixed, and observed using scanning electron microscopy. Once fine structures have been revealed by scanning electron microscopy, their component proteins may be identified by comparison with separately prepared fluorescence-labeled optical microscopic images of the candidate proteins, with their heavy-metal-labeled or stained ASEM images. Furthermore, cell nuclei in a tissue block stained with platinum-blue were successfully observed without thin-sectioning, which suggests the applicability of this inverted scanning electron microscope to cancer diagnosis. This microscope visualizes mesoscopic-scale structures, and is also applicable to non-bioscience fields including polymer chemistry. Copyright © 2010 Elsevier Inc. All rights reserved.

  17. Differential elastic electron scattering cross sections for CCl4 by 1.5-100 eV energy electron impact

    Science.gov (United States)

    Limão-Vieira, P.; Horie, M.; Kato, H.; Hoshino, M.; Blanco, F.; García, G.; Buckman, S. J.; Tanaka, H.

    2011-12-01

    We report absolute elastic differential, integral and momentum transfer cross sections for electron interactions with CCl4. The incident electron energy range is 1.5-100 eV, and the scattered electron angular range for the differential measurements varies from 15°-130°. The absolute scale of the differential cross section was set using the relative flow technique with helium as the reference species. Comparison with previous total cross sections shows good agreement. Atomic-like behaviour in this scattering system is shown here for the first time, and is further investigated by comparing the CCl4 elastic cross sections to recent results on the halomethanes and atomic chlorine at higher impact energies [H. Kato, T. Asahina, H. Masui, M. Hoshino, H. Tanaka, H. Cho, O. Ingólfsson, F. Blanco, G. Garcia, S. J. Buckman, and M. J. Brunger, J. Chem. Phys. 132, 074309 (2010)], 10.1063/1.3319761.

  18. Raising an Antibody Specific to Breast Cancer Subpopulations Using Phage Display on Tissue Sections

    DEFF Research Database (Denmark)

    Larsen, Simon Asbjørn; Meldgaard, Theresa; Fridriksdottir, Agla Jael Rubner

    2016-01-01

    BACKGROUND/AIM: Primary tumors display a great level of intra-tumor heterogeneity in breast cancer. The current lack of prognostic and predictive biomarkers limits accurate stratification and the ability to predict response to therapy. The aim of the present study was to select recombinant antibody...... fragments specific against breast cancer subpopulations, aiding the discovery of novel biomarkers. MATERIALS AND METHODS: Recombinant antibody fragments were selected by phage display. A novel shadowstick technology enabled the direct selection using tissue sections of antibody fragments specific against...

  19. Electrochemical removal of metallic implants from Technovit 9100 New embedded hard and soft tissues prior to histological sectioning.

    Science.gov (United States)

    Willbold, Elmar; Reebmann, Mattias; Jeffries, Richard; Witte, Frank

    2013-11-01

    Solid metallic implants in soft or hard tissues are serious challenges for histological processing. However, metallic implants are more frequently used in e.g. cardiovascular or orthopaedic therapies. Before clinical use, these devices need to be tested thoroughly in a biological environment and histological analysis of their biocompatibility is a major requirement. To allow the histological analysis of metallic implants in tissues especially in calcified hard tissues, we describe a method for embedding these tissues in the resin Technovit 9100 New and removing the metallic implants by electrochemical dissolution. With the combination of these two processes, we are able to achieve 5 μm thick sections from soft or hard tissues with a superior preservation of tissue architecture and especially the implant-tissue interface. These sections can be stained by classical stainings, immunohistochemical and enzymehistochemical as well as DNA-based staining methods.

  20. Improved calculation of displacements per atom cross section in solids by gamma and electron irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Piñera, Ibrahin, E-mail: ipinera@ceaden.edu.cu [Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear, CEADEN, 30 St. 502, Playa 11300, Havana (Cuba); Cruz, Carlos M.; Leyva, Antonio; Abreu, Yamiel; Cabal, Ana E. [Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear, CEADEN, 30 St. 502, Playa 11300, Havana (Cuba); Espen, Piet Van; Remortel, Nick Van [University of Antwerp, CGB, Groenenborgerlaan 171, 2020 Antwerpen (Belgium)

    2014-11-15

    Highlights: • We present a calculation procedure for dpa cross section in solids under irradiation. • Improvement about 10–90% for the gamma irradiation induced dpa cross section. • Improvement about 5–50% for the electron irradiation induced dpa cross section. • More precise results (20–70%) for thin samples irradiated with electrons. - Abstract: Several authors had estimated the displacements per atom cross sections under different approximations and models, including most of the main gamma- and electron-material interaction processes. These previous works used numerical approximation formulas which are applicable for limited energy ranges. We proposed the Monte Carlo assisted Classical Method (MCCM), which relates the established theories about atom displacements to the electron and positron secondary fluence distributions calculated from the Monte Carlo simulation. In this study the MCCM procedure is adapted in order to estimate the displacements per atom cross sections for gamma and electron irradiation. The results obtained through this procedure are compared with previous theoretical calculations. An improvement in about 10–90% for the gamma irradiation induced dpa cross section is observed in our results on regard to the previous evaluations for the studied incident energies. On the other hand, the dpa cross section values produced by irradiation with electrons are improved by our calculations in about 5–50% when compared with the theoretical approximations. When thin samples are irradiated with electrons, more precise results are obtained through the MCCM (in about 20–70%) with respect to the previous studies.

  1. Modification of PLGA Nanofibrous Mats by Electron Beam Irradiation for Soft Tissue Regeneration

    Directory of Open Access Journals (Sweden)

    Jae Baek Lee

    2015-01-01

    Full Text Available Biodegradable poly(lactide-co-glycolide (PLGA has found widespread use in modern medical practice. However, the degradation rate of PLGA should be adjusted for specific biomedical applications such as tissue engineering, drug delivery, and surgical implantation. This study focused on the effect of electron beam radiation on nanofibrous PLGA mats in terms of physical properties and degradation behavior with cell proliferation. PLGA nanofiber mats were prepared by electrospinning, and electron beam was irradiated at doses of 50, 100, 150, 200, 250, and 300 kGy. PLGA mats showed dimensional integrity after electron beam irradiation without change of fiber diameter. The degradation behavior of a control PLGA nanofiber (0 kGy and electron beam-irradiated PLGA nanofibers was analyzed by measuring the molecular weight, weight loss, change of chemical structure, and fibrous morphology. The molecular weight of the PLGA nanofibers decreased with increasing electron beam radiation dose. The mechanical properties of the PLGA nanofibrous mats were decreased with increasing electron beam irradiation dose. Cell proliferation behavior on all electron beam irradiated PLGA mats was similar to the control PLGA mats. Electron beam irradiation of PLGA nanofibrous mats is a potentially useful approach for modulating the biodegradation rate of tissue-specific nonwoven nanofibrous scaffolds, specifically for soft tissue engineering applications.

  2. Cross section for electronic energy transfer between mercury isotopes

    International Nuclear Information System (INIS)

    Lagushenko, R.; Grossman, M.W.; Maya, J.

    1984-01-01

    Previous estimates of the cross section (sigma) for the process 1 Hg(6 3 P 1 )+ /sup J/ Hg(6 1 S /sub o/ )→ 1 Hg(6 1 S /sub o/ )+ /sup J/ Hg(6 3 P 1 ), where i and j are any one of the six stable Hg isotopes, are no better than a factor of ten. We have recently measured the hyperfine structure of the 253.7nm Hg resonance line in low pressure Hg-Ar discharge for natural Hg as well as Hg enriched in 196 Hg by 2-4%, as a function of temperature. Using our previously developed resonance radiation transport model in a low pressure Hg+Ar plasma we calculated the exact shape of the hyperfine structure and found a high degree of sensitivity to the value of (sigma). By varying (sigma) we were able to obtain a best fit to the measured hyperfine structure as a function of temperature. This fit determined the value of (sigma). We believe the accuracy of this determination is about 30%

  3. Doubly differential cross sections for ionization of helium by electron impact

    International Nuclear Information System (INIS)

    Ray, H.; Werner, U.; Roy, A.C.

    1991-01-01

    The Glauber approximation is used to calculate doubly differential cross sections (DDCS's) for electron-impact ionization of helium at incident energies of 100, 300, and 500 eV. Angular dependences of the cross sections are presented for the primary (scattered) electrons. The present calculation is done for the case where the energy of the primary electron is large compared with that of the secondary (ejected) electron. A comparison is made of the present DDCS with the results of other calculations and experiment

  4. An automated segmentation methodology for quantifying immunoreactive puncta number and fluorescence intensity in tissue sections.

    Science.gov (United States)

    Fish, Kenneth N; Sweet, Robert A; Deo, Anthony J; Lewis, David A

    2008-11-13

    A number of human brain diseases have been associated with disturbances in the structure and function of cortical synapses. Answering fundamental questions about the synaptic machinery in these disease states requires the ability to image and quantify small synaptic structures in tissue sections and to evaluate protein levels at these major sites of function. We developed a new automated segmentation imaging method specifically to answer such fundamental questions. The method takes advantage of advances in spinning disk confocal microscopy, and combines information from multiple iterations of a fluorescence intensity/morphological segmentation protocol to construct three-dimensional object masks of immunoreactive (IR) puncta. This new methodology is unique in that high- and low-fluorescing IR puncta are equally masked, allowing for quantification of the number of fluorescently-labeled puncta in tissue sections. In addition, the shape of the final object masks highly represents their corresponding original data. Thus, the object masks can be used to extract information about the IR puncta (e.g., average fluorescence intensity of proteins of interest). Importantly, the segmentation method presented can be easily adapted for use with most existing microscopy analysis packages.

  5. Diagnosis of filamentous fungi on tissue sections by immunohistochemistry using anti-aspergillus antibody.

    Science.gov (United States)

    Challa, Sundaram; Uppin, Shantveer G; Uppin, Megha S; Pamidimukkala, Umabala; Vemu, Lakshmi

    2015-06-01

    Identification based on histology alone has limitations as Aspergillus species share morphology with other filamentous fungi. Differentiation of Aspergillus species from hyalohyphomycetes and dematiaceous fungi is important as the antifungal susceptibility varies among different species and genera. Given these problems, ancillary techniques are needed to increase specificity. Our aim was to study the utility of immunohistochemistry (IHC) with anti-Aspergillus antibody in the identification of Aspergillus species and to differentiate them from other filamentous fungi. Fifty formalin fixed, paraffin embedded tissue sections including 47 from cases of culture proven filamentous fungi, 3 from colonies of cultures of hyalohyphomycetes, and 11 smears from cultures were subjected to IHC studies using polyclonal rabbit anti-Aspergillus antibody (Abcam, UK) after antigen retrieval. The IHC on tissue sections was positive in 88% cases involving culture proven Aspergillus species. There was no cross reactivity with Mucorales species, Candida species, dematiaceous fungi and hyalohyphomycetes. Hence immunohistochemistry can be used as an ancillary technique for the diagnosis of Aspergillus species. © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses

    Directory of Open Access Journals (Sweden)

    Barjon Clément

    2012-07-01

    Full Text Available Abstract Background Galectin-9 is a mammalian lectin which possesses immunosuppressive properties. Excessive production of galectin-9 has been reported in two types of human virus-associated diseases chronic hepatitis C and nasopharyngeal carcinoma associated to the Epstein-Barr virus. The objective of this study was to produce new monoclonal antibodies targeting galectin-9 in order to improve its detection in clinical samples, especially on tissue sections analysed by immunohistochemistry. Methods Hybridomas were produced through immunization of mice with the recombinant c-terminus part of galectin-9 (residues 191 to 355 of the long isoform and semi-solid fusion of spleen cells with Sp2/0 cells. Monoclonal antibodies were characterized using ELISA, epitope mapping, western blot and immunohistochemistry. Results We selected seven hybridomas producing antibodies reacting with our recombinant c-terminus galectin-9 in ELISA. Five of them reacted with the epitope “TPAIPPMMYPHPA” (common to all isoforms, residues 210 to 222 of the long isoform and stained all three isoforms of galectin-9 analysed by western blot. One of them, 1G3,demonstrated very good sensitivity and specificity when used for immunohistochemistry. Using 1G3, we could confirm the intense and constant expression of galectin-9 by Epstein-Barr virus positive malignant cells from nasopharyngeal carcinomas. In most samples, specific staining was detected in both cytoplasm and nuclei. Galectin-9 was also detected in liver biopsies from patients infected by the human hepatitis C or B viruses with expression not only in inflammatory leucocytes and Kupffer cells, but also in hepatocytes. In contrast, galectin-9 was virtually absent in non-infected liver specimens. Conclusion The 1G3 monoclonal antibody will be a powerful tool to assess galectin-9 expression and distribution especially in diseases related to oncogenic viruses.

  7. Technical advances in the sectioning of dental tissue and of on-section cross-linked collagen detection in mineralized teeth.

    Science.gov (United States)

    Singhrao, Sim K; Sloan, Alastair J; Smith, Emma L; Archer, Charles W

    2010-08-01

    Immunohistochemical detection of cross-linked fibrillar collagens in mineralized tissues is much desired for exploring the mechanisms of biomineralization in health and disease. Mineralized teeth are impossible to section when embedded in conventional media, thus limiting on-section characterization of matrix proteins by immunohistochemistry. We hypothesized that by using an especially formulated acrylic resin suitable for mineralized dental tissues, not only sectioning of teeth would be possible, but also our recently developed immunofluorescence labeling technique would be amenable to fully calcified tissues for characterization of dentinal fibrillar collagens, which remains elusive. The hypothesis was tested on fixed rodent teeth embedded in Technovit 9100 New. It was possible to cut thin (1 mum) sections of mineralized teeth, and immunofluorescence characterization of cross-linked type I fibrillar collagen was selected due to its abundance in dentine. Decalcified samples of teeth embedded in paraffin wax were also used to compare immunolabeling from either method using the same immunoreagents in equivalent concentrations. In the decalcified tissue sections, type I collagen labeling in the dentine along the tubules was "patchy" and the signal in the predentine was very weak. However, enhanced signal in mineralized samples with type I collagen was detected not only in the predentine but also at the limit between intertubular dentine, within the elements of the enamel organ and subgingival stroma. This report offers advances in sectioning mineralized dental tissues and allows the application of immunofluorescence not only for on-section protein detection but importantly for detecting cross-linked fibrous collagens in both soft and mineralized tissue sections.

  8. Adhesion-governed buckling of thin-film electronics on soft tissues

    Directory of Open Access Journals (Sweden)

    Bo Wang

    2016-01-01

    Full Text Available Stretchable/flexible electronics has attracted great interest and attention due to its potentially broad applications in bio-compatible systems. One class of these ultra-thin electronic systems has found promising and important utilities in bio-integrated monitoring and therapeutic devices. These devices can conform to the surfaces of soft bio-tissues such as the epidermis, the epicardium, and the brain to provide portable healthcare functionalities. Upon contractions of the soft tissues, the electronics undergoes compression and buckles into various modes, depending on the stiffness of the tissue and the strength of the interfacial adhesion. These buckling modes result in different kinds of interfacial delamination and shapes of the deformed electronics, which are very important to the proper functioning of the bio-electronic devices. In this paper, detailed buckling mechanics of these thin-film electronics on elastomeric substrates is studied. The analytical results, validated by experiments, provide a very convenient tool for predicting peak strain in the electronics and the intactness of the interface under various conditions.

  9. Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue.

    Science.gov (United States)

    Carrascal, Mylène A; Talina, Catarina; Borralho, Paula; Gonçalo Mineiro, A; Henriques, Ana Raquel; Pen, Cláudia; Martins, Manuela; Braga, Sofia; Sackstein, Robert; Videira, Paula A

    2018-05-02

    The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLe X and sLe A ), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLe X and/or sLe A . However, antibody binding does not define E-selectin binding activity. In this study, we developed an immunohistochemical staining technique, using E-selectin-human Ig Fc chimera (E-Ig) to characterize the expression and localization of E-selectin binding sites on paraffin-embedded sections of different cancer tissue. E-Ig successfully stained cancer cells with high specificity. The E-Ig staining show high reactivity scores in colon and lung adenocarcinoma and moderate reactivity in triple negative breast cancer. Compared with reactivity of antibody against sLe X/A , the E-Ig staining presented higher specificity to cancer tissue with better defined borders and less background. The E-Ig staining technique allows the qualitative and semi-quantitative analysis of E-selectin binding activity on cancer cells. The development of accurate techniques for detection of selectin ligands may contribute to better diagnostic and better understanding of the molecular basis of tumor progression and metastasis.

  10. A compact and versatile microfluidic probe for local processing of tissue sections and biological specimens

    Science.gov (United States)

    Cors, J. F.; Lovchik, R. D.; Delamarche, E.; Kaigala, G. V.

    2014-03-01

    The microfluidic probe (MFP) is a non-contact, scanning microfluidic technology for local (bio)chemical processing of surfaces based on hydrodynamically confining nanoliter volumes of liquids over tens of micrometers. We present here a compact MFP (cMFP) that can be used on a standard inverted microscope and assist in the local processing of tissue sections and biological specimens. The cMFP has a footprint of 175 × 100 × 140 mm3 and can scan an area of 45 × 45 mm2 on a surface with an accuracy of ±15 μm. The cMFP is compatible with standard surfaces used in life science laboratories such as microscope slides and Petri dishes. For ease of use, we developed self-aligned mounted MFP heads with standardized "chip-to-world" and "chip-to-platform" interfaces. Switching the processing liquid in the flow confinement is performed within 90 s using a selector valve with a dead-volume of approximately 5 μl. We further implemented height-compensation that allows a cMFP head to follow non-planar surfaces common in tissue and cellular ensembles. This was shown by patterning different macroscopic copper-coated topographies with height differences up to 750 μm. To illustrate the applicability to tissue processing, 5 μm thick M000921 BRAF V600E+ melanoma cell blocks were stained with hematoxylin to create contours, lines, spots, gradients of the chemicals, and multiple spots over larger areas. The local staining was performed in an interactive manner using a joystick and a scripting module. The compactness, user-friendliness, and functionality of the cMFP will enable it to be adapted as a standard tool in research, development and diagnostic laboratories, particularly for the interaction with tissues and cells.

  11. Affinity imaging mass spectrometry (AIMS): high-throughput screening for specific small molecule interactions with frozen tissue sections.

    Science.gov (United States)

    Yoshimi, T; Kawabata, S; Taira, S; Okuno, A; Mikawa, R; Murayama, S; Tanaka, K; Takikawa, O

    2015-11-07

    A novel screening system, using affinity imaging mass spectrometry (AIMS), has been developed to identify protein aggregates or organ structures in unfixed human tissue. Frozen tissue sections are positioned on small (millimetre-scale) stainless steel chips and incubated with an extensive library of small molecules. Candidate molecules showing specific affinity for the tissue section are identified by imaging mass spectrometry (IMS). As an example application, we screened over a thousand compounds against Alzheimer's disease (AD) brain tissue and identified several compounds with high affinity for AD brain sections containing tau deposits compared to age-matched controls. It should also be possible to use AIMS to isolate chemical compounds with affinity for tissue structures or components that have been extensively modified by events such as oxidation, phosphorylation, acetylation, aggregation, racemization or truncation, for example, due to aging. It may also be applicable to biomarker screening programs.

  12. Cross section formulae on single W and Z boson productions in electron-positron collisions

    International Nuclear Information System (INIS)

    Katuya, Mituaki

    1987-01-01

    The formulae are given for the transverse momentum distributions and total cross sections for the single W boson and Z boson productions in electron-positron collisions by using the equivalent photon approximation. (author)

  13. Activity of flavonoids through λ sub max and electron cross-section

    International Nuclear Information System (INIS)

    Murthy, V.R.; Sreenivasulu, M.

    1997-01-01

    Flavonoids are widespread groups of natural constituents and present in the drugs in the form of mono or diglycosides. Being physiologically active, flavonoids are important in the field of pharmacological and clinical applications. Not many physical properties of flavonoids have been studied so far excepting UV and NMR studies. This paper deals with the evaluation of electron ionization cross section through λ sub max, a parameter available from UV studies. Electron ionization cross section was primarily conceived to be of use in radiation chemical data, mass spectroscopic and thermodynamic studies. But later attempts to correlate electron ionization cross-section with structural and related parameters prompted the authors to derive an explicit expression relating λ sub max and electron ionization cross-section (Q). The application of this method to correlate Q through λ sub max and interpret the results in terms of chemical activity are discussed

  14. Compilation of excitation cross sections for He atoms by electron impact

    International Nuclear Information System (INIS)

    Kato, T.; Itikawa, Y.; Sakimoto, K.

    1992-03-01

    Experimental and theoretical data are compiled on the cross section for the excitation of He atoms by electron impact. The available data are compared graphically. The survey of the literature has been made through the end 1991. (author)

  15. Radiation induced low-energy electron transport in a tissue environment

    International Nuclear Information System (INIS)

    Toburen, L.H.; Dingfelder, M.; Ozturk, N.; Christou, C.; Shinpaugh, J.L.; Friedland, W.; Wilson, W.E.; Paretzke, H.G.

    2003-01-01

    Monte Carlo (MC) track simulation codes are used extensively in radiobiology to quantify the spatial distributions of interactions initiated by the absorption of ionizing radiation. The spatial patterns of ionization and excitation are instrumental for assessing the formation of damage clusters in DNA and chromosomes leading to such biologic endpoints as cellular transformation and mutation. The MC codes rely on an extensive database of elastic and inelastic scattering cross sections to follow the production and slowing of secondary electrons. Because of inherent uncertainties in this database we are exploring the sensitivity of MC results to the details of the cross sections used with emphasis on low-energy electrons, i.e., track ends, that are anticipated to play a dominant role in damage cluster formation. Simulations of electron transport using gas or liquid based interaction cross sections illustrate substantial difference in the spectra of electrons with energies less than about 50 eV. In addition, the electron yields from MC simulations appear to be nearly a factor of five larger than our recent measurements of electron transport spectra in water (ice) at electron energies of about 10 eV. Examples of the changes in electron transport spectra for variations in the electron scattering cross sections used for the MC calculations will be illustrated and compared with an evolving database of measured spectra of electrons from ion induced secondary electron transport in thin foils. These measurements provide guidance for assessment of elastic and elastic cross sections appropriate to condensed phase transport. This work is supported in part by the U.S. Department of Energy, Grant No. DE-FG02-01ER-63233; the National Cancer Institute, Grant No. 1R01CA93351-01A1; and the European Community under Contract No. FIGH-CT-1999-00005

  16. Total cross section of furfural by electron impact: Experiment and theory.

    Science.gov (United States)

    Traoré Dubuis, A; Verkhovtsev, A; Ellis-Gibbings, L; Krupa, K; Blanco, F; Jones, D B; Brunger, M J; García, G

    2017-08-07

    We present experimental total cross sections for electron scattering from furfural in the energy range from 10 to 1000 eV, as measured using a double electrostatic analyzer gas cell electron transmission experiment. These results are compared to theoretical data for furfural, as well as to experimental and theoretical values for the structurally similar molecules furan and tetrahydrofuran. The measured total cross section is in agreement with the theoretical results obtained by means of the independent-atom model with screening corrected additivity rule including interference method. In the region of higher electron energies, from 500 eV to 10 keV, the total electron scattering cross section is also estimated using a semi-empirical model based on the number of electrons and dipole polarizabilities of the molecular targets. Together with the recently measured differential and integral cross sections, and the furfural energy-loss spectra, the present total cross section data nearly complete the data set that is required for numerical simulation of low-energy electron processes in furfural, covering the range of projectile energies from a few electron volts up to 10 keV.

  17. Total cross section of furfural by electron impact: Experiment and theory

    Science.gov (United States)

    Traoré Dubuis, A.; Verkhovtsev, A.; Ellis-Gibbings, L.; Krupa, K.; Blanco, F.; Jones, D. B.; Brunger, M. J.; García, G.

    2017-08-01

    We present experimental total cross sections for electron scattering from furfural in the energy range from 10 to 1000 eV, as measured using a double electrostatic analyzer gas cell electron transmission experiment. These results are compared to theoretical data for furfural, as well as to experimental and theoretical values for the structurally similar molecules furan and tetrahydrofuran. The measured total cross section is in agreement with the theoretical results obtained by means of the independent-atom model with screening corrected additivity rule including interference method. In the region of higher electron energies, from 500 eV to 10 keV, the total electron scattering cross section is also estimated using a semi-empirical model based on the number of electrons and dipole polarizabilities of the molecular targets. Together with the recently measured differential and integral cross sections, and the furfural energy-loss spectra, the present total cross section data nearly complete the data set that is required for numerical simulation of low-energy electron processes in furfural, covering the range of projectile energies from a few electron volts up to 10 keV.

  18. Exchange corrections to the bulk plasmon cross section of slow electrons in metals

    International Nuclear Information System (INIS)

    Yin, S.; Goodman, B.; Tosatti, E.

    1981-08-01

    We present a calculation of the simplest exchange correction to the low-energy electron cross section for plasmon creation in a free-electron-like metal with the density of Al. The resulting cancellation effect is found to be much too small to account for the anomalous delayed onset of plasmon losses, found in core level photo emission for Al. (author)

  19. New approximations of the differential electron-atom elastic scattering cross-sections

    International Nuclear Information System (INIS)

    Niculescu, V.I.R.; Catana, D.

    1994-01-01

    In the present note concerning the electron-atom interaction a cubic Spline method was used to obtain approximations of the differential cross-sections. These approximations gave a 20 times reduction of the computing time preserving also the accuracy (2%). The example is for Al in the 1-256 keV electron energy range. (Author) 2 Tabs., 3 Refs

  20. Prediction for neutrino-electron cross-sections in Weinberg's model for weak interactions

    NARCIS (Netherlands)

    Hooft, G. 't

    1971-01-01

    Weinberg's theory of purely leptonic weak interactions can be tested in neutrino-electron scattering experiments. Cross-sections must be measured as a function of the energy of the recoil electron. If Weinberg's theory is correct, then the masses of the intermediate vector bosons can be derived from

  1. Study of electron densities of normal and neoplastic human breast tissues by Compton scattering using synchrotron radiation

    International Nuclear Information System (INIS)

    Antoniassi, M.; Conceição, A.L.C.; Poletti, M.E.

    2012-01-01

    Electron densities of 33 samples of normal (adipose and fibroglangular) and neoplastic (benign and malignant) human breast tissues were determined through Compton scattering data using a monochromatic synchrotron radiation source and an energy dispersive detector. The area of Compton peaks was used to determine the electron densities of the samples. Adipose tissue exhibits the lowest values of electron density whereas malignant tissue the highest. The relationship with their histology was discussed. Comparison with previous results showed differences smaller than 4%. - Highlights: ► Electron density of normal and neoplastic breast tissues was measured using Compton scattering. ► Monochromatic synchrotron radiation was used to obtain the Compton scattering data. ► The area of Compton peaks was used to determine the electron densities of samples. ► Adipose tissue shows the lowest electron density values whereas the malignant tissue the highest. ► Comparison with previous results showed differences smaller than 4%.

  2. Antigen retrieval prior to on-tissue digestion of formalin-fixed paraffin-embedded tumour tissue sections yields oxidation of proline residues.

    Science.gov (United States)

    Djidja, Marie-Claude; Claude, Emmanuelle; Scriven, Peter; Allen, David W; Carolan, Vikki A; Clench, Malcolm R

    2017-07-01

    MALDI-mass spectrometry imaging (MALDI-MSI) has been shown to allow the study of protein distribution and identification directly within formalin-fixed paraffin-embedded (FFPE) tissue sections. However, direct protein identification from tissue sections remains challenging due to signal interferences and/or existing post-translational or other chemical modifications. The use of antigen retrieval (AR) has been demonstrated for unlocking proteins prior to in situ enzymatic digestion and MALDI-MSI analysis of FFPE tissue sections. In the work reported here, the identification of proline oxidation, which may occur when performing the AR protocol, is described. This facilitated and considerably increased the number of identified peptides when adding proline oxidation as a variable modification to the MASCOT search criteria. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Preparation of transmission electron microscopy cross-section specimens using focused ion beam milling

    International Nuclear Information System (INIS)

    Langford, R.M.; Petford-Long, A.K.

    2001-01-01

    The preparation of transmission electron microscopy cross-section specimens using focused ion beam milling is outlined. The 'liftout' and 'trench' techniques are both described in detail, and their relative advantages and disadvantages are discussed. Artifacts such as ion damage to the top surface and sidewalls of the cross-section specimens, and methods of reducing them, are addressed

  4. Measurements of integral cross-sections of incoherent interactions of photons with L-shell electrons

    Energy Technology Data Exchange (ETDEWEB)

    Verma, S L; Allawadhi, K L; Sood, B S [Punjabi Univ., Patiala (India). Nuclear Science Labs.

    1983-05-21

    Integral cross-sections of incoherent interactions of 662 and 1250 keV gamma-rays with L-shell electrons of different elements with 74<=Z<=92 have been measured. The experimental results, when interpreted in terms of photoelectric and Compton interaction cross-sections, are found to agree with theory.

  5. Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections.

    Directory of Open Access Journals (Sweden)

    Sandrine Prost

    Full Text Available The recent availability of novel dyes and alternative light sources to facilitate complex tissue immunofluorescence studies such as multiplex labelling has not been matched by reports critically evaluating the considerations and relative benefits of these new tools, particularly in combination. Product information is often limited to wavelengths used for older fluorophores (FITC, TRITC & corresponding Alexa dyes family. Consequently, novel agents such as Quantum dots are not widely appreciated or used, despite highly favourable properties including extremely bright emission, stability and potentially reduced tissue autofluorescence at the excitation wavelength. Using spectral analysis, we report here a detailed critical appraisal and comparative evaluation of different light sources and fluorophores in multiplex immunofluorescence of clinical biopsy sections. The comparison includes mercury light, metal halide and 3 different LED-based systems, using 7 Qdots (525, 565, 585, 605, 625, 705, Cy3 and Cy5. We discuss the considerations relevant to achieving the best combination of light source and fluorophore for accurate multiplex fluorescence quantitation. We highlight practical limitations and confounders to quantitation with filter-based approaches.

  6. Electron excitation cross sections for some Ar I 5d (J = 2) levels

    International Nuclear Information System (INIS)

    Blanco, F.; Sanchez, J.A.; Campos, J.

    1992-01-01

    Absolute excitation cross sections by electron impact for some 5d levels with J = 2 of Ar I have been measured by the optical method. Excitation functions for electron energies in the range from the excitation threshold to 1000 eV are also reported. A delayed coincidence analysis of the de-excitation at 100 eV electron energy allowed for the subtraction of radiative cascades. The resulting excitation cross sections are between 7.3 and 12x10 -20 cm 2 . (author)

  7. Calculation of total and ionization cross sections for electron scattering by primary benzene compounds

    Science.gov (United States)

    Singh, Suvam; Naghma, Rahla; Kaur, Jaspreet; Antony, Bobby

    2016-07-01

    The total and ionization cross sections for electron scattering by benzene, halobenzenes, toluene, aniline, and phenol are reported over a wide energy domain. The multi-scattering centre spherical complex optical potential method has been employed to find the total elastic and inelastic cross sections. The total ionization cross section is estimated from total inelastic cross section using the complex scattering potential-ionization contribution method. In the present article, the first theoretical calculations for electron impact total and ionization cross section have been performed for most of the targets having numerous practical applications. A reasonable agreement is obtained compared to existing experimental observations for all the targets reported here, especially for the total cross section.

  8. Calculation of total and ionization cross sections for electron scattering by primary benzene compounds

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Suvam; Naghma, Rahla; Kaur, Jaspreet; Antony, Bobby, E-mail: bka.ism@gmail.com [Atomic and Molecular Physics Lab, Department of Applied Physics, Indian School of Mines, Dhanbad (India)

    2016-07-21

    The total and ionization cross sections for electron scattering by benzene, halobenzenes, toluene, aniline, and phenol are reported over a wide energy domain. The multi-scattering centre spherical complex optical potential method has been employed to find the total elastic and inelastic cross sections. The total ionization cross section is estimated from total inelastic cross section using the complex scattering potential-ionization contribution method. In the present article, the first theoretical calculations for electron impact total and ionization cross section have been performed for most of the targets having numerous practical applications. A reasonable agreement is obtained compared to existing experimental observations for all the targets reported here, especially for the total cross section.

  9. Ionization of xenon by electrons: Partial cross sections for single, double, and triple ionization

    International Nuclear Information System (INIS)

    Mathur, D.; Badrinathan, C.

    1987-01-01

    High-sensitivity measurements of relative partial cross sections for single, double, and triple ionization of Xe by electron impact have been carried out in the energy region from threshold to 100 eV using a crossed-beam apparatus incorporating a quadrupole mass spectrometer. The weighted sum of the relative partial cross sections at 50 eV are normalized to the total ionization cross section of Rapp and Englander-Golden to yield absolute cross-section functions. Shapes of the partial cross sections for single and double ionization are difficult to account for within a single-particle picture. Comparison of the Xe + data with 4d partial photoionization cross-section measurements indicates the important role played by many-body effects in describing electron-impact ionization of high-Z atoms

  10. Absolute cross sections from the ''boomerang model'' for resonant electron-molecule scattering

    International Nuclear Information System (INIS)

    Dube, L.; Herzenberg, A.

    1979-01-01

    The boomerang model is used to calculate absolute cross sections near the 2 Pi/sub g/ shape resonance in e-N 2 scattering. The calculated cross sections are shown to satisfy detailed balancing. The exchange of electrons is taken into account. A parametrized complex-potential curve for the intermediate N 2 /sup ts-/ ion is determined from a small part of the experimental data, and then used to calculate other properties. The calculations are in good agreement with the absolute cross sections for vibrational excitation from the ground state, the absolute cross section v = 1 → 2, and the absolute total cross section

  11. Resonant ion-pair formation in the recombination of NO+ with electrons: Cross-section determination

    International Nuclear Information System (INIS)

    Le Padellec, A.; Djuric, N.; Al-Khalili, A.; Danared, H.; Derkatch, A. M.; Neau, A.; Popovic, D. B.; Rosen, S.; Semaniak, J.; Thomas, R.

    2001-01-01

    Resonant ion-pair formation from the collisions of NO + ions with electrons was studied using the heavy-ion storage ring CRYRING at the Manne Siegbahn Laboratory of Stockholm University. The total cross section is measured for the formation of N + +O - for electron energies 8--18 eV, and the results are compared with ion-pair formation in photoionization work. A peak in the cross section is observed at 12.5 eV, with a magnitude of 8.5 x 10 -19 cm 2 . An attempt to extract the cross section for the reverse process of associative ionization is made

  12. Single electron attachment and stripping cross sections for relativistic heavy ions

    International Nuclear Information System (INIS)

    Crawford, H.J.

    1979-06-01

    The results of a Bevalac experiment to measure the single electron attachment and stripping cross sections for relativistic (0.5 1 , and fully stripped, N 0 , ion beams emerging from the targets. Separate counters measured the number of ions in each charge state. The ratios N 1 /N 0 for different target thicknesses were fit to a simple growth curve to yield electron attachment and stripping cross sections. The data are compared to relativistic extrapolations of available theories. Clear evidence for two separate attachment processes, radiative and non-radiative, is found. Data are compared to a recently improved formulation for the stripping cross sections

  13. Total and elastic electron scattering cross sections from Xe at intermediate and high energies

    International Nuclear Information System (INIS)

    Garcia, G; Pablos, J L de; Blanco, F; Williart, A

    2002-01-01

    Experimental total electron scattering cross sections from Xe in the energy range 300-5000 eV have been obtained with experimental errors of about 3%. The method was based on the measurement of the attenuation of a linear electron beam through a Xe gas cell in combination with an electron spectroscopy technique to analyse the energy of the transmitted electrons. Differential and integral elastic cross sections have been calculated using a scattering potential method which includes relativistic effects. The consistency of our theoretical and experimental results is also discussed in the paper. Finally, analytical formulae depending on two parameters, namely the number of target electrons and the atomic polarizability, are given to reproduce the experimental data for Ne, Ar, Kr and Xe in the energy range 500-10 000 eV

  14. Engineered hybrid cardiac patches with multifunctional electronics for online monitoring and regulation of tissue function

    Science.gov (United States)

    Feiner, Ron; Engel, Leeya; Fleischer, Sharon; Malki, Maayan; Gal, Idan; Shapira, Assaf; Shacham-Diamand, Yosi; Dvir, Tal

    2016-06-01

    In cardiac tissue engineering approaches to treat myocardial infarction, cardiac cells are seeded within three-dimensional porous scaffolds to create functional cardiac patches. However, current cardiac patches do not allow for online monitoring and reporting of engineered-tissue performance, and do not interfere to deliver signals for patch activation or to enable its integration with the host. Here, we report an engineered cardiac patch that integrates cardiac cells with flexible, freestanding electronics and a 3D nanocomposite scaffold. The patch exhibited robust electronic properties, enabling the recording of cellular electrical activities and the on-demand provision of electrical stimulation for synchronizing cell contraction. We also show that electroactive polymers containing biological factors can be deposited on designated electrodes to release drugs in the patch microenvironment on demand. We expect that the integration of complex electronics within cardiac patches will eventually provide therapeutic control and regulation of cardiac function.

  15. Engineered hybrid cardiac patches with multifunctional electronics for online monitoring and regulation of tissue function

    Science.gov (United States)

    Feiner, Ron; Engel, Leeya; Fleischer, Sharon; Malki, Maayan; Gal, Idan; Shapira, Assaf; Shacham-Diamand, Yosi; Dvir, Tal

    2016-01-01

    In cardiac tissue engineering approaches to treat myocardial infarction, cardiac cells are seeded within three-dimensional porous scaffolds to create functional cardiac patches. However, current cardiac patches do not allow for online monitoring and reporting of engineered-tissue performance, and do not interfere to deliver signals for patch activation or to enable its integration with the host. Here, we report an engineered cardiac patch that integrates cardiac cells with flexible, free-standing electronics and a 3D nanocomposite scaffold. The patch exhibited robust electronic properties, enabling the recording of cellular electrical activities and the on-demand provision of electrical stimulation for synchronizing cell contraction. We also show that electroactive polymers containing biological factors can be deposited on designated electrodes to release drugs in the patch microenvironment on-demand. We expect that the integration of complex electronics within cardiac patches will eventually provide therapeutic control and regulation of cardiac function. PMID:26974408

  16. Differential cross sections for electron-impact vibrational-excitation of tetrahydrofuran at intermediate impact energies

    Energy Technology Data Exchange (ETDEWEB)

    Do, T. P. T. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia); School of Education, Can Tho University, Campus II, 3/2 Street, Xuan Khanh, Ninh Kieu, Can Tho City (Viet Nam); Duque, H. V. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia); Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); Lopes, M. C. A. [Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); Konovalov, D. A.; White, R. D. [College of Science, Technology and Engineering, James Cook University, Townsville (Australia); Brunger, M. J., E-mail: michael.brunger@flinders.edu.au, E-mail: darryl.jones@flinders.edu.au [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia); Institute of Mathematical Sciences, University of Malaya, 50603 Kuala Lumpur (Malaysia); Jones, D. B., E-mail: michael.brunger@flinders.edu.au, E-mail: darryl.jones@flinders.edu.au [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, South Australia 5001 (Australia)

    2015-03-28

    We report differential cross sections (DCSs) for electron-impact vibrational-excitation of tetrahydrofuran, at intermediate incident electron energies (15-50 eV) and over the 10°-90° scattered electron angular range. These measurements extend the available DCS data for vibrational excitation for this species, which have previously been obtained at lower incident electron energies (≤20 eV). Where possible, our data are compared to the earlier measurements in the overlapping energy ranges. Here, quite good agreement was generally observed where the measurements overlapped.

  17. Fine structures and ion images on fresh frozen dried ultrathin sections by transmission electron and scanning ion microscopy

    International Nuclear Information System (INIS)

    Takaya, K.; Okabe, M.; Sawataishi, M.; Takashima, H.; Yoshida, T.

    2003-01-01

    Ion microscopy (IM) of air-dried or freeze-dried cryostat and semi-thin cryosections has provided ion images of elements and organic substances in wide areas of the tissue. For reproducible ion images by a shorter time of exposure to the primary ion beam, fresh frozen dried ultrathin sections were prepared by freezing the tissue in propane chilled with liquid nitrogen, cryocut at 60 nm, mounted on grids and silicon wafer pieces, and freeze-dried. Rat Cowper gland and sciatic nerve, bone marrow of the rat administered of lithium carbonate, tree frog and African toad spleen and buffy coat of atopic dermatitis patients were examined. Fine structures and ion images of the corresponding areas in the same or neighboring sections were observed by transmission electron microscopy (TEM) followed by sector type and time-of-flight type IM. Cells in the buffy coat contained larger amounts of potassium and magnesium while plasma had larger amounts of sodium and calcium. However, in the tissues, lithium, sodium, magnesium, calcium and potassium were distributed in the cell and calcium showed a granular appearance. A granular cell of the tree frog spleen contained sodium and potassium over the cell and magnesium and calcium were confined to granules

  18. Electron tomography of HIV-1 infection in gut-associated lymphoid tissue.

    Science.gov (United States)

    Ladinsky, Mark S; Kieffer, Collin; Olson, Gregory; Deruaz, Maud; Vrbanac, Vladimir; Tager, Andrew M; Kwon, Douglas S; Bjorkman, Pamela J

    2014-01-01

    Critical aspects of HIV-1 infection occur in mucosal tissues, particularly in the gut, which contains large numbers of HIV-1 target cells that are depleted early in infection. We used electron tomography (ET) to image HIV-1 in gut-associated lymphoid tissue (GALT) of HIV-1-infected humanized mice, the first three-dimensional ultrastructural examination of HIV-1 infection in vivo. Human immune cells were successfully engrafted in the mice, and following infection with HIV-1, human T cells were reduced in GALT. Virions were found by ET at all stages of egress, including budding immature virions and free mature and immature viruses. Immuno-electron microscopy verified the virions were HIV-1 and showed CD4 sequestration in the endoplasmic reticulum of infected cells. Observation of HIV-1 in infected GALT tissue revealed that most HIV-1-infected cells, identified by immunolabeling and/or the presence of budding virions, were localized to intestinal crypts with pools of free virions concentrated in spaces between cells. Fewer infected cells were found in mucosal regions and the lamina propria. The preservation quality of reconstructed tissue volumes allowed details of budding virions, including structures interpreted as host-encoded scission machinery, to be resolved. Although HIV-1 virions released from infected cultured cells have been described as exclusively mature, we found pools of both immature and mature free virions within infected tissue. The pools could be classified as containing either mostly mature or mostly immature particles, and analyses of their proximities to the cell of origin supported a model of semi-synchronous waves of virion release. In addition to HIV-1 transmission by pools of free virus, we found evidence of transmission via virological synapses. Three-dimensional EM imaging of an active infection within tissue revealed important differences between cultured cell and tissue infection models and furthered the ultrastructural understanding of

  19. Electron tomography of HIV-1 infection in gut-associated lymphoid tissue.

    Directory of Open Access Journals (Sweden)

    Mark S Ladinsky

    2014-01-01

    Full Text Available Critical aspects of HIV-1 infection occur in mucosal tissues, particularly in the gut, which contains large numbers of HIV-1 target cells that are depleted early in infection. We used electron tomography (ET to image HIV-1 in gut-associated lymphoid tissue (GALT of HIV-1-infected humanized mice, the first three-dimensional ultrastructural examination of HIV-1 infection in vivo. Human immune cells were successfully engrafted in the mice, and following infection with HIV-1, human T cells were reduced in GALT. Virions were found by ET at all stages of egress, including budding immature virions and free mature and immature viruses. Immuno-electron microscopy verified the virions were HIV-1 and showed CD4 sequestration in the endoplasmic reticulum of infected cells. Observation of HIV-1 in infected GALT tissue revealed that most HIV-1-infected cells, identified by immunolabeling and/or the presence of budding virions, were localized to intestinal crypts with pools of free virions concentrated in spaces between cells. Fewer infected cells were found in mucosal regions and the lamina propria. The preservation quality of reconstructed tissue volumes allowed details of budding virions, including structures interpreted as host-encoded scission machinery, to be resolved. Although HIV-1 virions released from infected cultured cells have been described as exclusively mature, we found pools of both immature and mature free virions within infected tissue. The pools could be classified as containing either mostly mature or mostly immature particles, and analyses of their proximities to the cell of origin supported a model of semi-synchronous waves of virion release. In addition to HIV-1 transmission by pools of free virus, we found evidence of transmission via virological synapses. Three-dimensional EM imaging of an active infection within tissue revealed important differences between cultured cell and tissue infection models and furthered the ultrastructural

  20. Cross sections and spin polarizations of electrons elastically scattered from oriented molecules (CH3I)

    International Nuclear Information System (INIS)

    Fink, M.; Ross, A.W.; Fink, R.J.

    1989-01-01

    Elastic differential cross sections and spin polarizations for electrons elastically scattered from CH 3 I are calculated using the independent atom model. Three molecular orientations with respect to the incident electron wavevector are considered - first, the molecule is oriented randomly, second, the electron wave front and molecular bond are parallel, and third, the wavefront and the bond axis are perpendicular. It will be seen to what extent orientational averaging weakens features of the cross section and spin polarization. The calculations show that cross section and spin polarization measurements are a possible tool for determining the degree of molecular orientation. There is no degeneracy between I-C and C-I in cross section and spin polarization measurements. The results presented here for 200 eV and 600 eV electrons scattered by CH 3 I should be considered as a case study and it should be possible to find molecules and electron energies for which even more dramatic differences between the various orientations between the molecules and the electrons can be expected. (orig.)

  1. Differential cross sections for intermediate-energy electron scattering from α-tetrahydrofurfuryl alcohol: Excitation of electronic-states

    Energy Technology Data Exchange (ETDEWEB)

    Chiari, L.; Jones, D. B.; Thorn, P. A.; Pettifer, Z. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Duque, H. V. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Departamento de Física, Universidade Federal de Juiz de Fora, Juiz de Fora, MG (Brazil); Silva, G. B. da [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso (Brazil); Limão-Vieira, P. [Laboratório de Colisões Atómicas e Moleculares, CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Duflot, D. [Laboratoire de Physique des Lasers, Atomes et Molécules, UMR CNRS 8523, Université Lille, F-59655 Villeneuve d’Ascq Cedex (France); Hubin-Franskin, M.-J.; Delwiche, J. [Départment de Chimie, Université de Liège, Institut de Chimie-Bât. B6C, B-4000 Liège 1 (Belgium); Blanco, F. [Departamento de Física Atómica, Molecular y Nuclear, Universidad Complutense de Madrid, Madrid E-28040 (Spain); García, G. [Instituto de Física Fundamental, CSIC, Madrid E-28006 (Spain); and others

    2014-07-14

    We report on measurements of differential cross sections (DCSs) for electron impact excitation of a series of Rydberg electronic-states in α-tetrahydrofurfuryl alcohol (THFA). The energy range of these experiments was 20–50 eV, while the scattered electron was detected in the 10°–90° angular range. There are currently no other experimental data or theoretical computations against which we can directly compare the present measured results. Nonetheless, we are able to compare our THFA DCSs with earlier cross section measurements for Rydberg-state electronic excitation for tetrahydrofuran, a similar cyclic ether, from Do et al. [J. Chem. Phys. 134, 144302 (2011)]. In addition, “rotationally averaged” elastic DCSs, calculated using our independent atom model with screened additivity rule correction approach are also reported. Those latter results give integral cross sections consistent with the optical theorem, and supercede those from the only previous study of Milosavljević et al. [Eur. Phys. J. D 40, 107 (2006)].

  2. Measurements of secondary electron cross sections by the pulsed electron beam time-of-flight method. I. Molecular nitrogen

    International Nuclear Information System (INIS)

    Goruganthu, R.R.; Wilson, W.G.; Bonham, R.A.

    1983-01-01

    The secondary electron cross sections for gaseous molecular nitrogen are reported at ejection angles of 30, 45, 60, 75, 90, 105, 120, 135 and 150 0 , for the energy range 1.5 eV to 20 eV and incident electron energy of 1 keV. The pulsed electron beam time-of-flight methd was employed. The results were placed on an absolute scale by normalization to the elastic scattering. They were compared, where possible, with those reported by Opal, Beaty, and Peterson (OBP). The agreement is somewhat better when the OBP data are divided by 0.53 + 0.47 sintheta as suggested by Rudd and DuBois. Fits of our data by Legendre-polynomial expansions are used to estimate the low-energy portion of the cross-section, dsigma/dE. This work suggests that existing experimental cross sections for secondary electron ejection as a function of angle and ejected energy may be no better known than +-40%, especially in the low energy region. 7 references, 14 figures, 2 tables

  3. Comment on 'Integral cross sections for electron impact excitation of electronic states of N2'

    International Nuclear Information System (INIS)

    Cenian, A; Chernukho, A; Bogaerts, A; Gijbels, R

    2002-01-01

    Based on the existing experimental data and their statistical errors, it is not possible to make a sound recommendation of the cross-section set of Campbell et al (J. Phys. B: At. Mol. Opt. Phys. 34 (2001) 1185). We comment on this paper. (comment)

  4. Electron loss process and cross section of multiply charged ions by neutral atoms

    International Nuclear Information System (INIS)

    Karashima, S.; Watanabe, T.

    1985-01-01

    The significance of experimental and theoretical results on the electron loss and capture of ions in matter plays an important role in the charge equilibrium problems of fusion plasma physics and of accelerator physics. In the report, we calculate electron stripping cross section by using the binary encounter approximation (BEA). Our treatment of the electron loss process is based on BEA, in which the nucleus of B screened by the surrounding electrons collides with electrons in the ion A sup(q+). The basic approximation in EBA is that the ion interacts with only one electron or nucleus of the target atom at a time. In the calculation for Li sup(2+) + H, we have found that EBA will give approximately reliable results. (Mori, K.)

  5. Calculated Cross Sections for the Electron Impact Ionization of Molecular Ions

    Science.gov (United States)

    Deutsch, H.; Becker, K.; Defrance, P.; Onthong, U.; Parajuli, R.; Probst, M.; Matt-Leubner, S.; Maerk, T.

    2002-10-01

    We report the results of the application of the semi- classical Deutsch-Märk (DM) formalism to the calculation of the absolute electron-impact ionization cross section of the molecular ions H2+, N2+, O2+, CD+, CO+, CO2+, H3O+, and CH4+ for which experimental data have been reported . Where available, we also compare our calculated cross sections with calculated cross sections using the BEB method of Kim and co-workers. The level of agreement between the experimentally determined and calculated cross section is satisfactory in some cases. In all cases, the calculated cross sections exceed the measured cross sections which is not surprising in view of the experimental complications in measuring ionization cross sections of molecular ions due to the presence of competing channels such as ionization dissociative ionization, and dissociative excitation. Work supported in part by FWF, OEAW, and NASA.

  6. Relativistic total and differential cross section proton--proton electron--positron pair production calculation

    International Nuclear Information System (INIS)

    Rubinstein, J.E.

    1976-01-01

    Circle Feynman diagrams for a specific permutation of variables along with their corresponding algebraic expressions are presented to evaluate [H] 2 for proton-proton electron-positron pair production. A Monte Carlo integration technique is introduced and is used to set up the multiple integral expression for the total pair production cross section. The technique is first applied to the Compton scattering problem and then to an arbitrary multiple integral. The relativistic total cross section for proton-proton electron-positron pair production was calculated for eight different values of incident proton energy. A variety of differential cross sections were calculated for the above energies. Angular differential cross section distributions are presented for the electron, positron, and proton. Invariant mass differential cross section distributions are done both with and without the presence of [H] 2 . Both WGHT and log 10 (TOTAL) distributions were also obtained. The general behavioral trends of the total and differential cross sections for proton-proton electron-positron pair production are presented. The range of validity for this calculation is from 0 to about 200 MeV

  7. Measurements of Neutron Induced Cross Sections at the Oak Ridge Electron Linear Accelerator

    International Nuclear Information System (INIS)

    Guber, K.H.; Harvey, J.A.; Hill, N.W.; Koehler, P.E.; Leal, L.C.; Sayer, R.O.; Spencer, R.R.

    1999-01-01

    We have used the Oak Ridge Electron Linear Accelerator (ORELA) to measure neutron total and the fission cross sections of 233 U in the energy range from 0.36 eV to 700 keV. We report average fission and total cross sections. Also, we measured the neutron total cross sections of 27 Al and Natural chlorine as well as the capture cross section of Al over an energy range from 100 eV up to about 400 keV

  8. Establishing the impact of temporary tissue expanders on electron and photon beam dose distributions.

    Science.gov (United States)

    Asena, A; Kairn, T; Crowe, S B; Trapp, J V

    2015-05-01

    This study investigates the effects of temporary tissue expanders (TTEs) on the dose distributions in breast cancer radiotherapy treatments under a variety of conditions. Using EBT2 radiochromic film, both electron and photon beam dose distribution measurements were made for different phantoms, and beam geometries. This was done to establish a more comprehensive understanding of the implant's perturbation effects under a wider variety of conditions. The magnetic disk present in a tissue expander causes a dose reduction of approximately 20% in a photon tangent treatment and 56% in electron boost fields immediately downstream of the implant. The effects of the silicon elastomer are also much more apparent in an electron beam than a photon beam. Evidently, each component of the TTE attenuates the radiation beam to different degrees. This study has demonstrated that the accuracy of photon and electron treatments of post-mastectomy patients is influenced by the presence of a tissue expander for various beam orientations. The impact of TTEs on dose distributions establishes the importance of an accurately modelled high-density implant in the treatment planning system for post-mastectomy patients. Copyright © 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

  9. Micro-Raman spectroscopy a powerful technique to identify crocidolite and erionite fibers in tissue sections

    Science.gov (United States)

    Rinaudo, C.; Croce, A.; Allegrina, M.; Baris, I. Y.; Dogan, A.; Powers, A.; Rivera, Z.; Bertino, P.; Yang, H.; Gaudino, G.; Carbone, M.

    2013-05-01

    Exposure to mineral fibers such asbestos and erionite is widely associated with the development of lung cancer and pleural malignant mesothelioma (MM). Pedigree and mineralogical studies indicated that genetics may influence mineral fiber carcinogenesis. Although dimensions strongly impact on the fiber carcinogenic potential, also the chemical composition and the fiber is relevant. By using micro-Raman spectroscopy we show here persistence and identification of different mineral phases, directly on histopathological specimens of mice and humans. Fibers of crocidolite asbestos and erionite of different geographic areas (Oregon, US and Cappadocia, Turkey) were injected in mice intra peritoneum. MM developed in 10/15 asbestos-treated mice after 5 months, and in 8-10/15 erionite-treated mice after 14 months. The persistence of the injected fibers was investigated in pancreas, liver, spleen and in the peritoneal tissue. The chemical identification of the different phases occurred in the peritoneal cavity or at the organ borders, while only rarely fibers were localized in the parenchyma. Raman patterns allow easily to recognize crocidolite and erionite fibers. Microscopic analysis revealed that crocidolite fibers were frequently coated by ferruginous material ("asbestos bodies"), whereas erionite fibers were always free from coatings. We also analyzed by micro-Raman spectroscopy lung tissues, both from MM patients of the Cappadocia, where a MM epidemic developed because of environmental exposure to erionite, and from Italian MM patients with occupational exposure to asbestos. Our findings demonstrate that micro-Raman spectroscopy is technique able to identify mineral phases directly on histopathology specimens, as routine tissue sections prepared for diagnostic purpose. REFERENCES A.U. Dogan, M. Dogan. Environ. Geochem. Health 2008, 30(4), 355. M. Carbone, S. Emri, A.U. Dogan, I. Steele, M. Tuncer, HI. Pass, et al. Nat. Rev. Cancer. 2007, 7 (2),147. M. Carbone, Y

  10. Deconvolution of overlapping features in electron energy-loss spectra: the determination of absolute differential cross sections for electron-impact excitation of electronic states of molecules

    International Nuclear Information System (INIS)

    Campbell, L.; Brunger, M.J.; Teubner, O.J.P.; Mojarrabi, B.

    1996-06-01

    A set of three computer programs is reported which allow for the deconvolution of overlapping molecular electronic state structure in electron energy-loss spectra, even in highly perturbed systems. This procedure enables extraction of absolute differential cross sections for electron-impact excitation of electronic states of diatomic molecules from electron energy-loss spectra. The first code in the sequence uses the Rydberg-Klein-Rees procedure to generate potential energy curves from spectroscopic constants, while the second calculates Franck-Condon factors by numerical solution of the Schroedinger equation, given the potential energy curves. The third, given these Franck-Condon factors, the previously calculated relevant energies for the vibrational levels of the respective electronic states and the experimental energy-loss spectra, extracts the differential cross sections for each state. Each program can be run independently, or the three can run in sequence to determine these cross sections from the spectroscopic constants and the experimental energy-loss spectra. The application of these programs to the specific case of electron scattering from nitric oxide (NO) is demonstrated. 25 refs., 2 tabs., 2 figs

  11. Diagnostic utility of melanin production by fungi: Study on tissue sections and culture smears with Masson-Fontana stain

    Directory of Open Access Journals (Sweden)

    Challa Sundaram

    2014-01-01

    Full Text Available Background: Dematiaceous fungi appear brown in tissue section due to melanin in their cell walls. When the brown color is not seen on routine H and E and culture is not available, differentiation of dematiaceous fungi from other fungi is difficult on morphology alone. Aims and Objective: To study if melanin production by dematiaceous fungi can help differentiate them from other types of fungi. Materials and Methods: Fifty tissue sections of various fungal infections and 13 smears from cultures of different species of fungi were stained with Masson Fontana stain to assess melanin production. The tissue sections included biopsies from 26 culture-proven fungi and 24 biopsies of filamentous fungi diagnosed on morphology alone with no culture confirmation. Results: All culture-proven dematiaceous fungi and Zygomycetes showed strong positivity in sections and culture smears. Aspergillus sp showed variable positivity and intensity. Cryptococcus neoformans showed strong positivity in tissue sections and culture smears. Tissue sections of septate filamentous fungi (9/15, Zygomycetes (4/5, and fungi with both hyphal and yeast morphology (4/4 showed positivity for melanin. The septate filamentous fungi negative for melanin were from biopsy samples of fungal sinusitis including both allergic and invasive fungal sinusitis and colonizing fungal balls. Conclusion: Melanin is produced by both dematiaceous and non-dematiaceous fungi. Masson-Fontana stain cannot reliably differentiate dematiaceous fungi from other filamentous fungi like Aspergillus sp; however, absence of melanin in the hyphae may be used to rule out dematiaceous fungi from other filamentous fungi. In the differential diagnosis of yeast fungi, Cryptococcus sp can be differentiated from Candida sp by Masson-Fontana stain in tissue sections.

  12. Total electron scattering cross sections for methanol and ethanol at intermediate energies

    International Nuclear Information System (INIS)

    Silva, D G M; Tejo, T; Lopes, M C A; Muse, J; Romero, D; Khakoo, M A

    2010-01-01

    Absolute total cross section (TCS) measurements of electron scattering from gaseous methanol and ethanol molecules are reported for impact energies from 60 to 500 eV, using the linear transmission method. The attenuation of intensity of a collimated electron beam through the target volume is used to determine the absolute TCS for a given impact energy, using the Beer-Lambert law to first approximation. Besides these experimental measurements, we have also determined TCS using the additivity rule.

  13. Differential cross sections for single-electron capture in He{sup 2+}-D collisions

    Energy Technology Data Exchange (ETDEWEB)

    Bordenave-Montesquieu, D.; Dagnac, R. [Centre National de la Recherche Scientifique (CNRS), 31 - Toulouse (France)]|[Toulouse-3 Univ., 31 (France)

    1995-06-14

    A translational energy spectroscopy technique was used to study single-electron capture into the He{sup +} (n = 2) and He{sup +} (n 3) states in He{sup 2+}-D collisions. Differential cross sections were determined at 4, 6 and 8 keV in the angular range 5`-1{sup o}30` (laboratory frame). As expected, single-electron capture into the n = 2 state was found to be the dominant process; total cross sections for capture into the He{sup +} (n = 3) state were compared to other experimental and theoretical results. (author).

  14. Ionization of molecules by electron impact: Differential and total cross sections

    Energy Technology Data Exchange (ETDEWEB)

    Rezkallah, Z. [Laboratoire de Physique Quantique et Systemes Dynamiques, Departement de physique, Faculte des sciences, Universite Ferhat Abbas, Setif 19000 (Algeria); Houamer, S., E-mail: hosalim@yahoo.com [Laboratoire de Physique Quantique et Systemes Dynamiques, Departement de physique, Faculte des sciences, Universite Ferhat Abbas, Setif 19000 (Algeria); Dal Cappello, C. [Laboratoire de Physique Moleculaire et des Collisions, Universite Paul Verlaine-Metz, Institut de Physique, 1 Boulevard Arago, 57078 Metz Cedex 3 (France); Charpentier, I. [Laboratoire de Physique et Mecanique des Materiaux, Universite Paul Verlaine-Metz UMR 7554, ile du Saulcy, 57045 Metz Cedex 1 (France); Roy, A.C. [School of Mathematical Sciences, Ramakrishna Mission Vivekananda University, Belur Math 711202, West Bengal (India)

    2011-12-01

    The first Born approximation is applied to calculate differential and total ionization cross sections of a set of small molecules, namely, HF, H{sub 2}O, NH{sub 3} and CH{sub 4} by electron impact. The molecular targets are described by single center molecular orbitals consisting of linear combinations of atomic orbitals (MO-LCAO). First, we have considered electron momentum spectroscopy experiments to check the accuracy of the wave functions. The triply, doubly, singly differential and total cross sections are then evaluated in a systematic way for a variety of kinematics. The results are discussed and compared with experiments.

  15. Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections.

    Science.gov (United States)

    Ververis, Katherine; Marzully, Selly; Samuel, Chrishan S; Hewitson, Tim D; Karagiannis, Tom C

    2016-01-01

    Fluorescent microscope imaging technologies are increasing in their applications and are being used on a wide scale. However methods used to quantify the level of fluorescence intensity are often not utilized-perhaps given the result may be immediately seen, quantification of the data may not seem necessary. However there are a number of reasons given to quantify fluorescent images including the importance of removing potential bias in the data upon observation as well as quantification of large numbers of images gives statistical power to detect subtle changes in experiments. In addition discreet localization of a protein could be detected without selection bias that may not be detectable by eye. Such data will be deemed useful when detecting the levels of HDAC enzymes within cells in order to develop more effective HDAC inhibitor compounds for use against multiple diseased states. Hence, we discuss a methodology devised to analyze fluorescent images using Image J to detect the mean fluorescence intensity of the 11 metal-dependent HDAC enzymes using murine kidney tissue sections as an example.

  16. Peripheral laser iridoplasty opens angle in plateau iris by thinning the cross-sectional tissues

    Directory of Open Access Journals (Sweden)

    Liu J

    2013-09-01

    Full Text Available Ji Liu,1,2 Tania Lamba,1 David A Belyea1 1Department of Ophthalmology, The George Washington University, Washington DC, USA; 2Yale Eye Center, Yale University, New Haven, CT, USA Abstract: Plateau iris syndrome has been described as persistent angle narrowing or occlusion with intraocular pressure elevation after peripheral iridotomy due to the abnormal plateau iris configuration. Argon laser peripheral iridoplasty (ALPI is an effective adjunct procedure to treat plateau iris syndrome. Classic theory suggests that the laser causes the contraction of the far peripheral iris stroma, "pulls" the iris away from the angle, and relieves the iris-angle apposition. We report a case of plateau iris syndrome that was successfully treated with ALPI. Spectral domain optical coherence tomography confirmed the angle was open at areas with laser treatment but remained appositionally closed at untreated areas. Further analysis suggested significant cross-sectional thinning of the iris at laser-treated areas in comparison with untreated areas. The findings indicate that APLI opens the angle, not only by contracting the iris stroma, but also by thinning the iris tissue at the crowded angle. This is consistent with the ALPI technique to aim at the iris as far peripheral as possible. This case also suggests that spectral domain optical coherence tomography is a useful adjunct imaging tool to gonioscopy in assessing the angle condition. Keywords: plateau iris, optic coherence tomography, argon laser peripheral iridoplasty, angle-closure glaucoma

  17. Spatial accuracy of 3D reconstructed radioluminographs of serial tissue sections and resultant absorbed dose estimates

    Energy Technology Data Exchange (ETDEWEB)

    Petrie, I.A.; Flynn, A.A.; Pedley, R.B.; Green, A.J.; El-Emir, E.; Dearling, J.L.J.; Boxer, G.M.; Boden, R.; Begent, R.H.J. [Cancer Research UK Targeting and Imaging Group, Department of Oncology, Royal Free and University College Medical School, Royal Free Campus, London (United Kingdom)

    2002-10-21

    Many agents using tumour-associated characteristics are deposited heterogeneously within tumour tissue. Consequently, tumour heterogeneity should be addressed when obtaining information on tumour biology or relating absorbed radiation dose to biological effect. We present a technique that enables radioluminographs of serial tumour sections to be reconstructed using automated computerized techniques, resulting in a three-dimensional map of the dose-rate distribution of a radiolabelled antibody. The purpose of this study is to assess the reconstruction accuracy. Furthermore, we estimate the potential error resulting from registration misalignment, for a range of beta-emitting radionuclides. We compare the actual dose-rate distribution with that obtained from the same activity distribution but with manually defined translational and rotational shifts. As expected, the error produced with the short-range {sup 14}C is much larger than that for the longer range {sup 90}Y; similarly values for the medium range {sup 131}I are between the two. Thus, the impact of registration inaccuracies is greater for short-range sources. (author)

  18. Theoretical and experimental study on electron interactions with chlorobenzene: Shape resonances and differential cross sections

    Energy Technology Data Exchange (ETDEWEB)

    Barbosa, Alessandra Souza [Departamento de Física, Universidade Federal do Paraná, CP 19044, 81531-990 Curitiba, Paraná (Brazil); Laboratório de Colisões Atómicas e Moleculares, CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Varella, Márcio T. do N. [Instituto de Física, Universidade de São Paulo, Rua do Matão 1731, 05508-090 São Paulo, SP (Brazil); Sanchez, Sergio d’A.; Bettega, Márcio H. F., E-mail: bettega@fisica.ufpr.br [Departamento de Física, Universidade Federal do Paraná, CP 19044, 81531-990 Curitiba, Paraná (Brazil); Ameixa, João; Limão-Vieira, Paulo; Ferreira da Silva, Filipe [Laboratório de Colisões Atómicas e Moleculares, CEFITEC, Departamento de Física, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Blanco, Francisco [Departamento de Física Atómica, Molecular y Nuclear, Facultad de Ciencias Físicas, Universidad Complutense de Madrid, E-28040 Madrid (Spain); and others

    2016-08-28

    In this work, we report theoretical and experimental cross sections for elastic scattering of electrons by chlorobenzene (ClB). The theoretical integral and differential cross sections (DCSs) were obtained with the Schwinger multichannel method implemented with pseudopotentials (SMCPP) and the independent atom method with screening corrected additivity rule (IAM-SCAR). The calculations with the SMCPP method were done in the static-exchange (SE) approximation, for energies above 12 eV, and in the static-exchange plus polarization approximation, for energies up to 12 eV. The calculations with the IAM-SCAR method covered energies up to 500 eV. The experimental differential cross sections were obtained in the high resolution electron energy loss spectrometer VG-SEELS 400, in Lisbon, for electron energies from 8.0 eV to 50 eV and angular range from 7{sup ∘} to 110{sup ∘}. From the present theoretical integral cross section (ICS) we discuss the low-energy shape-resonances present in chlorobenzene and compare our computed resonance spectra with available electron transmission spectroscopy data present in the literature. Since there is no other work in the literature reporting differential cross sections for this molecule, we compare our theoretical and experimental DCSs with experimental data available for the parent molecule benzene.

  19. A semi-empirical formula for total cross sections of electron scattering from diatomic molecules

    International Nuclear Information System (INIS)

    Liu Yufang; Sun Jinfeng; Henan Normal Univ., Xinxiang

    1996-01-01

    A fitting formula based on the Born approximation is used to fit the total cross sections for electron scattering by diatomic molecules (CO, N 2 , NO, O 2 and HCl) in the intermediate- and high-energy range. By analyzing the fitted parameters and the total cross sections, we found that the internuclear distance of the constituent atoms plays an important role in the e-diatomic molecule collision process. Thus a new semi-empirical formula has been obtained. There is no free parameter in the formula, and the dependence of the total cross sections on the internuclear distance has been reflected clearly. The total cross sections for electron scattering by CO, N 2 , NO, O 2 and HCl have been calculated over an incident energy range of 10-4000 eV. The results agree well with other available experimental and calculation data. (orig.)

  20. Energy and angle differential cross sections for the electron-impact double ionization of helium

    International Nuclear Information System (INIS)

    Colgan, James P.; Pindzola, M.S.; Robicheaux, F.

    2008-01-01

    Energy and angle differential cross sections for the electron-impact double ionization of helium are calculated using a non-perturbative time-dependent close-coupling method. Collision probabilities are found by projection of a time evolved nine dimensional coordinate space wave function onto fully antisymmetric products of spatial and spin functions representing three outgoing Coulomb waves. At an incident energy of 106 eV, we present double energy differential cross sections and pentuple energy and angle differential cross sections. The pentuple energy and angle differential cross sections are found to be in relative agreement with the shapes observed in recent (e,3e) reaction microscope experiments. Integration of the differential cross sections over all energies and angles yields a total ionization cross section that is also in reasonable agreement with absolute crossed-beams experiments.

  1. Electron capture cross-section of Au-Fe complex in silicon

    International Nuclear Information System (INIS)

    Ali, Akbar; Shafi, M; Majid, Abdul

    2006-01-01

    A deep level transient spectroscopy technique is applied to study the capture cross-section of an iron-gold complex. The thermal ionization energy obtained from emission rate data is found to be E c -0.36 eV. The Au-Fe complex is a single defect having a capture cross-section of 2.48x10 -16 cm 2 for electrons which is independent of temperature

  2. Uncertainties of Electron Capture Cross Sections In Be4+ + H(1s) Collisions

    International Nuclear Information System (INIS)

    Méndez, L.; Illescas, Clara; Jorge, Alba; Errea, L.F.; Rabadán, I.; Suárez, J.

    2014-01-01

    We have considered one-electron systems where the theoretical methods are well established. The use of different computational alternatives enables the accurate evaluation of nl-partial cross sections in a wide range of collision energies. In the presentation we have analyzed the uncertainties of n-partial charge exchange (CX) cross sections in Be 4+ + H(1s) collisions, which are relevant in tokamak plasmas and experimental data are not available.

  3. High-frequency two-electron photoionization cross section of triplet states

    International Nuclear Information System (INIS)

    Krivec, R.; Amusia, M.Ya.; Mandelzweig, V.B.

    2003-01-01

    Using high precision wave functions describing the triplet ground and excited 3 S states of the He atom and heliumlike ions, the cross sections of single- and double-electron photoionization are calculated. The dependence of the ratio R of the double and single ionization cross sections on the nuclear charge Z and the principal quantum number of excitation n is studied. The results obtained are compared to those for previously studied singlet states

  4. Electron capture cross-section of Au-Fe complex in silicon

    Energy Technology Data Exchange (ETDEWEB)

    Ali, Akbar; Shafi, M; Majid, Abdul [Advance Materials Physics Laboratory, Department of Physics, Quaid-i-Azam University, Islamabad (Pakistan)

    2006-10-15

    A deep level transient spectroscopy technique is applied to study the capture cross-section of an iron-gold complex. The thermal ionization energy obtained from emission rate data is found to be E{sub c} -0.36 eV. The Au-Fe complex is a single defect having a capture cross-section of 2.48x10{sup -16} cm{sup 2} for electrons which is independent of temperature.

  5. Bibliography of low energy electron and photon cross section data (through December 1974). Final report

    International Nuclear Information System (INIS)

    Kieffer, L.J.

    1976-03-01

    A bibliography of original reports of measurements or calculations of electron, positron and photon cross sections and their ions is presented. A detailed index to the bibliography allows retrieval of cross section data for specific processes and atomic or molecular species. A comprehensive author index is included. The bibliography covers the period 1921 through calendar year 1974, but some references late in 1974 may not have been found and included. No references were found prior to 1921

  6. Bibliography of low energy electron and photon cross section data. Report for Jan 1975-Dec 1977

    International Nuclear Information System (INIS)

    Gallagher, J.W.; Rumble, J.R. Jr; Beaty, E.C.

    1979-06-01

    A bibliography of original reports of measurements or calculations of electron, positron, and photon cross sections for atoms, small molecules, and their ions is presented. A detailed index to the bibliography facilitates retrieval of cross section data for specific processes and associated atomic or molecular species. A comprehensive author index is included. The bibliography covers the calendar years 1975-1977. This work supplements a previous bibliography which covered the literature through 1974

  7. Theoretical calculation of fully differential cross sections for electron-impact ionization of hydrogen molecules

    International Nuclear Information System (INIS)

    Gao Junfang; Madison, D H; Peacher, J L

    2006-01-01

    We have recently proposed the orientation averaged molecular orbital (OAMO) approximation for calculating fully differential cross sections (FDCS) for electron-impact ionization of molecules averaged over all molecular orientations. Orientation averaged FDCS were calculated for electron-impact ionization of nitrogen molecules using the distorted wave impulse approximation (DWIA) and the molecular three-body distorted wave (M3DW) approximation. In this paper, we use the same methods to examine the FDCS for ionization of hydrogen molecules. It is found that the DWIA yields reasonable results for high-energy incident electrons. While the DWIA breaks down for low-energy electrons, the M3DW gives reasonable results down to incident-electron energies around 35 eV

  8. A high-resolution optical imaging system for obtaining the serial transverse section images of biologic tissue

    Science.gov (United States)

    Wu, Li; Zhang, Bin; Wu, Ping; Liu, Qian; Gong, Hui

    2007-05-01

    A high-resolution optical imaging system was designed and developed to obtain the serial transverse section images of the biologic tissue, such as the mouse brain, in which new knife-edge imaging technology, high-speed and high-sensitive line-scan CCD and linear air bearing stages were adopted and incorporated with an OLYMPUS microscope. The section images on the tip of the knife-edge were synchronously captured by the reflection imaging in the microscope while cutting the biologic tissue. The biologic tissue can be sectioned at interval of 250 nm with the same resolution of the transverse section images obtained in x and y plane. And the cutting job can be automatically finished based on the control program wrote specially in advance, so we save the mass labor of the registration of the vast images data. In addition, by using this system a larger sample can be cut than conventional ultramicrotome so as to avoid the loss of the tissue structure information because of splitting the tissue sample to meet the size request of the ultramicrotome.

  9. The irradiation action on human dental tissue by X-rays and electrons. A nanoindenter study

    Energy Technology Data Exchange (ETDEWEB)

    Fraenzel, Wolfgang [Halle-Wittenberg Univ., Halle (Germany). Dept. of Physics; Gerlach, Reinhard [Halle-Wittenberg Univ., Halle (Germany). Clinic of Radiation Therapy

    2009-07-01

    It is known that ionizing radiation is used in medicine for Roentgen diagnostics and for radiation therapy. The radiation interacts with matter, in particular with biological one, essentially by scattering, photoelectric effect, Compton effect and pair production. To what extent the biological material is changed thereby, depends on the type and the amount of radiation energy, on the dose and on the tissue constitution. In modern radiation therapy two different kinds of radiation are used: high energy X-rays and electron radiation. In the case of head-neck tumors the general practice is an irradiation with high energy X-rays with absorbed dose to water up to 70 Gy. Teeth destruction has been identified as a side effect during irradiation. In addition, damage to the salivary glands is often observed which leads to a decrease or even the complete loss of the salivary secretion (xerostomia). This study shows how the different energy and radiation types damage the tooth tissue. The effects of both, high X-ray energy and high energy electrons, on the mechanical properties hardness and elasticity of the human dental tissue are measured by the nanoindentation technique. We compare these results with the effect of the irradiation of low X-ray energy on the dental tissue. (orig.)

  10. The irradiation action on human dental tissue by X-rays and electrons. A nanoindenter study

    International Nuclear Information System (INIS)

    Fraenzel, Wolfgang; Gerlach, Reinhard

    2009-01-01

    It is known that ionizing radiation is used in medicine for Roentgen diagnostics and for radiation therapy. The radiation interacts with matter, in particular with biological one, essentially by scattering, photoelectric effect, Compton effect and pair production. To what extent the biological material is changed thereby, depends on the type and the amount of radiation energy, on the dose and on the tissue constitution. In modern radiation therapy two different kinds of radiation are used: high energy X-rays and electron radiation. In the case of head-neck tumors the general practice is an irradiation with high energy X-rays with absorbed dose to water up to 70 Gy. Teeth destruction has been identified as a side effect during irradiation. In addition, damage to the salivary glands is often observed which leads to a decrease or even the complete loss of the salivary secretion (xerostomia). This study shows how the different energy and radiation types damage the tooth tissue. The effects of both, high X-ray energy and high energy electrons, on the mechanical properties hardness and elasticity of the human dental tissue are measured by the nanoindentation technique. We compare these results with the effect of the irradiation of low X-ray energy on the dental tissue. (orig.)

  11. Collision cross sections for few electron systems. Final report, August 1, 2992--July 31, 1995

    International Nuclear Information System (INIS)

    Reading, J.F.; Ford, A.L.

    1995-01-01

    The purpose of this project was to produce accurate cross sections for collisionally induced reactions from the ground stated and excited states of species of ions and at present in a hot fusion plasma. The collisional constituents may be divided into two categories for the purpose of calculations: Those in which a bare projectile excites a one electron or two electron ion or atom from its ground state, or excited states to higher excited states or ionized states. Those in which the projectile has one or more electrons attached to it and excites a one electron or two electron ion or atom from its ground state, or excited states to higher excited states or ionized states. During the collision the projectile itself may change its state being simultaneously excited or ionized. Cross sections are needed typically over the whole energy range from low velocities where molecular, orbitals begin to form to high velocities where first Born or more sophisticated asymptotic theories can be used. These high energy cross sections are very useful for experimentalists to check the absolute normalization of their cross sections. The theoretical tools used were therefore both analytical and numerical in character. Numerical calculations were restricted to expansions of the wavefunctions in a set of finite hilbert basis states (FHBS). The many body aspects of the problem, i.e. the important presence of the interelectron force, or correlation mandate a careful systematic approach. But this section was tempered in our strategy by the fact that many of the cross sections needed, especially from excited states, have never been calculated or measured at all. Thus any information we can provide is useful even if later work may modify our results

  12. Measurements of electron-proton elastic cross sections for 0.4 2 2

    International Nuclear Information System (INIS)

    Christy, M.E.; Ahmidouch, Abdellah; Armstrong, Christopher; Arrington, John; Razmik Asaturyan; Steven Avery; Baker, O.; Douglas Beck; Henk Blok; Bochna, C.W.; Werner Boeglin; Peter Bosted; Maurice Bouwhuis; Herbert Breuer; Brown, D.S.; Antje Bruell; Roger Carlini; Nicholas Chant; Anthony Cochran; Leon Cole; Samuel Danagoulian; Donal Day; James Dunne; Dipangkar Dutta; Rolf Ent; Howard Fenker; Fox, B.; Liping Gan; Haiyan Gao; Kenneth Garrow; David Gaskell; Ashot Gasparian; Don Geesaman; Paul Gueye; Mark Harvey; Roy Holt; Xiaodong Jiang; Cynthia Keppel; Edward Kinney; Yongguang Liang; Wolfgang Lorenzon; Allison Lung; Pete Markowitz; Martin, J.W.; Kevin McIlhany; Daniella Mckee; David Meekins; Miller, J.W.; Richard Milner; Joseph Mitchell; Hamlet Mkrtchyan; Robert Mueller; Alan Nathan; Gabriel Niculescu; Maria-Ioana Niculescu; Thomas O'neill; Vassilios Papavassiliou; Stephen Pate; Buz Piercey; David Potterveld; Ronald Ransome; Joerg Reinhold; Rollinde, E.; Philip Roos; Adam Sarty; Reyad Sawafta; Elaine Schulte; Edwin Segbefia; Smith, C.; Stepan Stepanyan; Steffen Strauch; Vardan Tadevosyan; Liguang Tang; Raphael Tieulent; Alicia Uzzle; William Vulcan; Stephen Wood; Feng Xiong; Lulin Yuan; Markus Zeier; Benedikt Zihlmann; Vitaliy Ziskin

    2004-01-01

    We report on precision measurements of the elastic cross section for electron-proton scattering performed in Hall C at Jefferson Lab. The measurements were made at 28 unique kinematic settings covering a range in momentum transfer of 0.4 2 2 . These measurements represent a significant contribution to the world's cross section data set in the Q 2 range where a large discrepancy currently exists between the ratio of electric to magnetic proton form factors extracted from previous cross section measurements and that recently measured via polarization transfer in Hall A at Jefferson Lab

  13. The momentum transfer cross section and transport coefficients for low energy electrons in mercury

    International Nuclear Information System (INIS)

    McEachran, R P; Elford, M T

    2003-01-01

    The momentum transfer cross section for electrons incident on mercury atoms has been determined from the solution of Dirac-Fock scattering equations which included both static and dynamic multipole polarization potentials as well as full anti-symmetrization to incorporate exchange effects. This cross section is in excellent agreement between 0.2 and 3.0 eV with the cross section derived from the most recent experimental measurements. The discrepancy below 0.2 eV has been investigated using two-term transport theory

  14. Strategy for alignment of electron beam trajectory in LEReC cooling section

    Energy Technology Data Exchange (ETDEWEB)

    Seletskiy, S. [Brookhaven National Lab. (BNL), Upton, NY (United States); Blaskiewicz, M. [Brookhaven National Lab. (BNL), Upton, NY (United States); Fedotov, A. [Brookhaven National Lab. (BNL), Upton, NY (United States); Kayran, D. [Brookhaven National Lab. (BNL), Upton, NY (United States); Kewisch, J. [Brookhaven National Lab. (BNL), Upton, NY (United States); Michnoff, R. [Brookhaven National Lab. (BNL), Upton, NY (United States); Pinayev, I. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2016-09-23

    We considered the steps required to align the electron beam trajectory through the LEReC cooling section. We devised a detailed procedure for the beam-based alignment of the cooling section solenoids. We showed that it is critical to have an individual control of each CS solenoid current. Finally, we modeled the alignment procedure and showed that with two BPM fitting the solenoid shift can be measured with 40 um accuracy and the solenoid inclination can be measured with 30 urad accuracy. These accuracies are well within the tolerances of the cooling section solenoid alignment.

  15. Intermediate energy cross sections for electron-impact vibrational-excitation of pyrimidine

    Energy Technology Data Exchange (ETDEWEB)

    Jones, D. B. [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Ellis-Gibbings, L.; García, G. [Instituto de Física Fundamental, CSIC, Serrano 113-bis, 28006 Madrid (Spain); Nixon, K. L. [Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); School of Biology, Chemistry and Forensic Science, University of Wolverhampton, Wolverhampton WV1 1LY (United Kingdom); Lopes, M. C. A. [Departamento de Física, Universidade Federal de Juiz de Fora, 36036-330 Juiz de Fora, Minas Gerais (Brazil); Brunger, M. J., E-mail: Michael.Brunger@flinders.edu.au [School of Chemical and Physical Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001 (Australia); Institute of Mathematical Sciences, University of Malaya, 50603 Kuala Lumpur (Malaysia)

    2015-09-07

    We report differential cross sections (DCSs) and integral cross sections (ICSs) for electron-impact vibrational-excitation of pyrimidine, at incident electron energies in the range 15–50 eV. The scattered electron angular range for the DCS measurements was 15°–90°. The measurements at the DCS-level are the first to be reported for vibrational-excitation in pyrimidine via electron impact, while for the ICS we extend the results from the only previous condensed-phase study [P. L. Levesque, M. Michaud, and L. Sanche, J. Chem. Phys. 122, 094701 (2005)], for electron energies ⩽12 eV, to higher energies. Interestingly, the trend in the magnitude of the lower energy condensed-phase ICSs is much smaller when compared to the corresponding gas phase results. As there is no evidence for the existence of any shape-resonances, in the available pyrimidine total cross sections [Baek et al., Phys. Rev. A 88, 032702 (2013); Fuss et al., ibid. 88, 042702 (2013)], between 10 and 20 eV, this mismatch in absolute magnitude between the condensed-phase and gas-phase ICSs might be indicative for collective-behaviour effects in the condensed-phase results.

  16. Electron-detachment cross sections of halogen negative-ion projectiles for inertial confinement fusion

    Science.gov (United States)

    Sant'Anna, M. M.; Zappa, F.; Santos, A. C. F.; de Barros, A. L. F.; Wolff, W.; Coelho, L. F. S.; de Castro Faria, N. V.

    2004-07-01

    Negative-ion beams have recently been suggested as sources of high-energy heavy atoms to be used as drivers for inertial confinement fusion (ICF). Owing to their electron affinities limited to a few eV, anions can be efficiently photo-detached in the vicinity of the fusion chamber, with the resulting high-velocity neutral projectiles following ballistic trajectories towards the hydrogen pellet target. Electron-detachment cross sections are needed as parameters to estimate the beam attenuation in the path from the ion source to the hydrogen pellet. Halogen anions are possible projectile choices. In this paper we present experimental data for total electron-detachment cross sections for F-, Cl-, Br- and I- ions incident on N2, in the 0.94-74 keV u-1 energy range. Our measurements can benchmark theory on anion electron detachment at intermediate to high velocities. Comparison between different projectiles shows very similar collision velocity dependencies. A simple geometrical scaling is presented, providing an estimate for electron-detachment cross sections at the MeV u-1 energy range. The presented scaling indicates that the vacuum requirements due to the use of halogen anions for ICF are less critical than previously suggested.

  17. Electron-detachment cross sections of halogen negative-ion projectiles for inertial confinement fusion

    International Nuclear Information System (INIS)

    Sant'Anna, M M; Zappa, F; Santos, A C F; Barros, A L F de; Wolff, W; Coelho, L F S; Faria, N V de Castro

    2004-01-01

    Negative-ion beams have recently been suggested as sources of high-energy heavy atoms to be used as drivers for inertial confinement fusion (ICF). Owing to their electron affinities limited to a few eV, anions can be efficiently photo-detached in the vicinity of the fusion chamber, with the resulting high-velocity neutral projectiles following ballistic trajectories towards the hydrogen pellet target. Electron-detachment cross sections are needed as parameters to estimate the beam attenuation in the path from the ion source to the hydrogen pellet. Halogen anions are possible projectile choices. In this paper we present experimental data for total electron-detachment cross sections for F - , Cl - , Br - and I - ions incident on N 2 , in the 0.94-74 keV u -1 energy range. Our measurements can benchmark theory on anion electron detachment at intermediate to high velocities. Comparison between different projectiles shows very similar collision velocity dependencies. A simple geometrical scaling is presented, providing an estimate for electron-detachment cross sections at the MeV u -1 energy range. The presented scaling indicates that the vacuum requirements due to the use of halogen anions for ICF are less critical than previously suggested

  18. Differential cross sections for the one electron two center symmetric systems

    International Nuclear Information System (INIS)

    Maidagan, J.M.; Piacentini, R.D.; Rivarola, R.D.; Universidad Autonoma de Madrid

    1982-01-01

    We use the two-state atomic expansion with variable nuclear charge to study charge-exchange differential cross sections for symmetrical one-electron systems at intermediate energy. The nonclassical small angle diffraction scattering is discussed. Our results are compared with data for H + -H collisions. (orig.)

  19. Differential cross sections for the one electron two center symmetric systems

    Energy Technology Data Exchange (ETDEWEB)

    Maidagan, J.M.; Piacentini, R.D. (Universidad Nacional de Rosario (Argentina). Dept. de Fisica); Rivarola, R.D. (Bordeaux-1 Univ., 33 - Talence (France). Lab. d' Astrophysique; Universidad Autonoma de Madrid (Spain). Dept. de Quimica Fisica y Quimica Cuantica)

    1982-03-01

    We use the two-state atomic expansion with variable nuclear charge to study charge-exchange differential cross sections for symmetrical one-electron systems at intermediate energy. The nonclassical small angle diffraction scattering is discussed. Our results are compared with data for H/sup +/-H collisions.

  20. Experimental and theoretical electron-scattering cross-section data for dichloromethane

    Science.gov (United States)

    Krupa, K.; Lange, E.; Blanco, F.; Barbosa, A. S.; Pastega, D. F.; Sanchez, S. d'A.; Bettega, M. H. F.; García, G.; Limão-Vieira, P.; Ferreira da Silva, F.

    2018-04-01

    We report on a combination of experimental and theoretical investigations into the elastic differential cross sections (DCSs) and integral cross sections for electron interactions with dichloromethane, C H2C l2 , in the incident electron energy over the 7.0-30 eV range. Elastic electron-scattering cross-section calculations have been performed within the framework of the Schwinger multichannel method implemented with pseudopotentials (SMCPP), and the independent-atom model with screening-corrected additivity rule including interference-effects correction (IAM-SCAR+I). The present elastic DCSs have been found to agree reasonably well with the results of IAM-SCAR+I calculations above 20 eV and also with the SMC calculations below 30 eV. Although some discrepancies were found for 7 eV, the agreement between the two theoretical methodologies is remarkable as the electron-impact energy increases. Calculated elastic DCSs are also reported up to 10000 eV for scattering angles from 0° to 180° together with total cross section within the IAM-SCAR+I framework.

  1. Measurement of integral cross-sections of incoherent interactions of photons with K-shell electrons

    Energy Technology Data Exchange (ETDEWEB)

    Verma, S L; Allawadhi, K L; Sood, B S [Punjabi Univ., Patiala (India). Dept. of Physics. Nuclear Science Labs.

    1981-06-01

    Integral cross-sections of incoherent interactions of 145, 279, 662 and 1250 keV gamma-rays with K-shell electrons of thirty-one different elements with 26 <= Z <= 92 have been measured. The results are interpreted in terms of the photoelectric and Compton interactions and are found to agree with theory.

  2. The LXCat project: Electron scattering cross sections and swarm parameters for low temperature plasma modeling

    International Nuclear Information System (INIS)

    Pancheshnyi, S.; Biagi, S.; Bordage, M.C.; Hagelaar, G.J.M.; Morgan, W.L.; Phelps, A.V.; Pitchford, L.C.

    2012-01-01

    Graphical abstract: LXCat is an open-access website containing data needed for low temperature plasma modeling as well as on-line tools useful for their manipulation. Highlights: ► LXCat: an open-access website with data for low temperature plasma modeling. ► Contains compilations of electron scattering cross sections and transport data. ► Data from different contributors for many neutral, ground-state species. ► On-line tools for browsing, plotting, up/downloading data. ► On-line Boltzmann solver for calculating electron swarm parameters. - Abstract: LXCat is a dynamic, open-access, website for collecting, displaying, and downloading ELECtron SCATtering cross sections and swarm parameters (mobility, diffusion coefficient, reaction rates, etc.) required for modeling low temperature, non-equilibrium plasmas. Contributors set up individual databases, and the available databases, indicated by the contributor’s chosen title, include mainly complete sets of electron-neutral scattering cross sections, although the option for introducing partial sets of cross sections exists. A database for measured swarm parameters is also part of LXCat, and this is a growing activity. On-line tools include options for browsing, plotting, and downloading cross section data. The electron energy distribution functions (edfs) in low temperature plasmas are in general non-Maxwellian, and LXCat provides an option for execution of an on-line Boltzmann equation solver to calculate the edf in homogeneous electric fields. Thus, the user can obtain electron transport and rate coefficients (averages over the edfs) in pure gases or gas mixtures over a range of values of the reduced electric fields strength, E/N, the ratio of the electric field strength to the neutral density, using cross sections from the available databases. New contributors are welcome and anyone wishing to create a database and upload data can request a username and password. LXCat is part of a larger, community

  3. Measurements of Electron Proton Elastic Cross Sections for 0.4

    International Nuclear Information System (INIS)

    Christy, M.E.; Abdellah Ahmidouch; Christopher Armstrong; John Arrington; Arshak Asaturyan; Steven Avery; Baker, O.; Douglas Beck; Henk Blok; Bochna, C.W.; Werner Boeglin; Peter Bosted; Maurice Bouwhuis; Herbert Breuer; Brown, D.S.; Antje Bruell; Roger Carlini; Nicholas Chant; Anthony Cochran; Leon Cole; Samuel Danagoulian; Donal Day; James Dunne; Dipangkar Dutta; Rolf Ent; Howard Fenker; Fox, B.; Liping Gan; Haiyan Gao; Kenneth Garrow; David Gaskell; Ashot Gasparian; Don Geesaman; Paul Gueye; Mark Harvey; Roy Holt; Xiaodong Jiang; Cynthia Keppel; Edward Kinney; Yongguang Liang; Wolfgang Lorenzon; Allison Lung; Pete Markowitz; Martin, J.W.; Kevin Mcilhany; David Mckee; David Meekins; Miller, M.A.; Richard Milner; Joseph Mitchell; Hamlet Mkrtchyan; Robert Mueller; Alan Nathan; Gabriel Niculescu; Maria-ioana Niculescu; Thomas O'neill; Vassilios Papavassiliou; Stephen Pate; Rodney Piercey; David Potterveld; Ronald Ransome; Joerg Reinhold; Rollinde, E.; Philip Roos; Adam Sarty; Reyad Sawafta; Elaine Schulte; Edwin Segbefia; Smith, C.; Samuel Stepanyan; Steffen Strauch; Vardan Tadevosyan; Liguang Tang; Raphael Tieulent; Alicia Uzzle; William Vulcan; Stephen Wood; Feng Xiong; Lulin Yuan; Markus Zeier; Benedikt Zihlmann; Vitaliy Ziskin

    2004-01-01

    We report on precision measurements of the elastic cross section for electron-proton scattering performed in Hall C at Jefferson Lab. The measurements were made at 28 distinct kinematic settings covering a range in momentum transfer of 0.4 < Q2 < 5.5 (GeV/c)2. These measurements represent a significant contribution to the world's cross section data set in the Q2 range, where a large discrepancy currently exists between the ratio of electric to magnetic proton form factors extracted from previous cross section measurements and that recently measured via polarization transfer in Hall A at Jefferson Lab. This data set shows good agreement with previous cross section measurements, indicating that if a heretofore unknown systematic error does exist in the cross section measurements, then it is intrinsic to all such measurements

  4. Preservation of high resolution protein structure by cryo-electron microscopy of vitreous sections

    International Nuclear Information System (INIS)

    Sader, Kasim; Studer, Daniel; Zuber, Benoit; Gnaegi, Helmut; Trinick, John

    2009-01-01

    We have quantitated the degree of structural preservation in cryo-sections of a vitrified biological specimen. Previous studies have used sections of periodic specimens to assess the resolution present, but preservation before sectioning was not assessed and so the damage due particularly to cutting was not clear. In this study large single crystals of lysozyme were vitrified and from these X-ray diffraction patterns extending to better than 2.1 A were obtained. The crystals were high pressure frozen in 30% dextran, and cryo-sectioned using a diamond knife. In the best case, preservation to a resolution of 7.9 A was shown by electron diffraction, the first observation of sub-nanometre structural preservation in a vitreous section.

  5. Electron-impact excitation and ionization cross sections for ground state and excited helium atoms

    International Nuclear Information System (INIS)

    Ralchenko, Yu.; Janev, R.K.; Kato, T.; Fursa, D.V.; Bray, I.; Heer, F.J. de

    2008-01-01

    Comprehensive and critically assessed cross sections for the electron-impact excitation and ionization of ground state and excited helium atoms are presented. All states (atomic terms) with n≤4 are treated individually, while the states with n≥5 are considered degenerate. For the processes involving transitions to and from n≥5 levels, suitable cross section scaling relations are presented. For a large number of transitions, from both ground and excited states, convergent close coupling calculations were performed to achieve a high accuracy of the data. The evaluated/recommended cross section data are presented by analytic fit functions, which preserve the correct asymptotic behavior of the cross sections. The cross sections are also displayed in graphical form

  6. Experimental determination of electron shock excitation cross sections for a singly charged gadolinium ion

    International Nuclear Information System (INIS)

    Smirnov, Yu.M.

    1995-01-01

    The trends observed in the processes of excitation with simultaneous ionization have received little study. This is particularly so for rare-earth elements having electron shells of complex structure and optical spectra very rich in lines. Among the basic factors responsible for such a situation, we should mention two: the difficulty presented by theoretical analysis of the processes discussed and the absence of factual information about the excitation cross sections with simultaneous ionization for the majority of rare-earth elements. The aim of the present work is to investigate the excitation of a singly charged gadolinium ion in the collisions of monokinetic electrons with gadolinium atoms. Up to the present time, only the excitation cross sections of a gadolinium atom have been measured, where investigation of the electron shock excitation of gadolinium atoms in their free state is associated with overcoming large experimental difficulties. About 160 crosss sections for the excitation of a singly charged gadolinium ion were measured and for a third of the cross sections; the energy dependences were recorded for the change in energy of the elecrons from the excitation threshold up to 200 eV. Included are tables of the wavelength, transistion, internal quantum number, the energy of the lowere and upper levels, and the values of cross sections for the charged gadolinium ion. Diagrams of the transistion energy states of Gd (II) and spectroscopy are presented and explained

  7. Absolute differential cross sections for elastic scattering of electrons by helium, neon, argon and molecular nitrogen

    International Nuclear Information System (INIS)

    Jansen, R.H.J.; De Heer, F.J.; Luyken, H.J.; Van Wingerden, B.

    1976-01-01

    An electron spectrometer has been constructed for the study of elastic and inelastic electron scattering processes. Up to now the apparatus has been used to measure differential cross sections of electrons elastically scattered by He, Ne, Ar and N 2 . Direct absolute cross section measurements were performed on N 2 at 500 eV impact energy and at scattering angles between 5 0 and 9 0 . Relative cross section measurements were done on He, Ne, Ar and N 2 at impact energies between 100 and 3000 eV and scattering angles between 5 0 and 55 0 . The relative cross sections were put on an absolute scale by means of the apparatus calibration factor derived from the absolute measurements on N 2 . The experimental apparatus and procedure are described in detail. The results are discussed and compared with those of other experimental and theoretical groups. Analysis of the exponential behaviour of the differential cross section as a function of momentum transfer yielded apparent polarizabilities of the target. (author)

  8. Differential cross sections for elastic scattering of electrons by atoms and solids

    International Nuclear Information System (INIS)

    Jablonski, A.; Salvat, F.; Powell, C.J.

    2004-01-01

    Differential cross sections (DCSs) for elastic scattering of electrons by neutral atoms are extensively used in studies of electron transport in solids and liquids. A new NIST database has recently been released with DCSs calculated from a relativistic Dirac partial-wave analysis in which the potentials were obtained from Dirac-Hartree-Fock electron densities computed self-consistently for free atoms. We have compared calculated DCSs with measured DCSs for argon for electron energies between 50 eV and 3 keV, and found good agreement for electron energies above about 1 keV but with increasing deviations as the energy is reduced. These deviations are due to the neglect of absorption and polarizability effects in the calculations. Nevertheless, DCSs for neutral atoms have been successfully used in simulations of elastic backscattering of electrons by solid surfaces with energies down to 300 eV as well as for many other applications. It is suggested that this success might be due at least partially to the smaller absorption correction for the DCSs in solids on account of the smaller total inelastic scattering cross sections than for the corresponding free atoms

  9. In Situ Blotting : A Novel Method for Direct Transfer of Native Proteins from Sectioned Tissue to Blotting Membrane

    NARCIS (Netherlands)

    Okabe, Masashi; Nyakas, Csaba; Buwalda, Bauke; Luiten, Paul G.M.

    1993-01-01

    We describe a novel technique for direct transfer of native proteins from unfixed frozen tissue sections to an immobilizing matrix, e.g., nitrocellulose, polyvinyliden difluoride, or positively charged nylon membranes. Proteins are directly blotted onto the membrane, providing optimal accessibility

  10. In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections

    Science.gov (United States)

    Rust fungi infect a wide range of plant species making them of particular interest to plant pathologists. In order to study the interactions between these important pathogenic fungi and their host plants it is useful to be able to differentiate fungal tissue from plant tissue. This can be accomplish...

  11. A new laser reflectance system capable of measuring changing cross-sectional area of soft tissues during tensile testing.

    Science.gov (United States)

    Pokhai, Gabriel G; Oliver, Michele L; Gordon, Karen D

    2009-09-01

    Determination of the biomechanical properties of soft tissues such as tendons and ligaments is dependent on the accurate measurement of their cross-sectional area (CSA). Measurement methods, which involve contact with the specimen, are problematic because soft tissues are easily deformed. Noncontact measurement methods are preferable in this regard, but may experience difficulty in dealing with the complex cross-sectional shapes and glistening surfaces seen in soft tissues. Additionally, existing CSA measurement systems are separated from the materials testing machine, resulting in the inability to measure CSA during testing. Furthermore, CSA measurements are usually made in a different orientation, and with a different preload, prior to testing. To overcome these problems, a noncontact laser reflectance system (LRS) was developed. Designed to fit in an Instron 8872 servohydraulic test machine, the system measures CSA by orbiting a laser transducer in a circular path around a soft tissue specimen held by tissue clamps. CSA measurements can be conducted before and during tensile testing. The system was validated using machined metallic specimens of various shapes and sizes, as well as different sizes of bovine tendons. The metallic specimens could be measured to within 4% accuracy, and the tendons to within an average error of 4.3%. Statistical analyses showed no significant differences between the measurements of the LRS and those of the casting method, an established measurement technique. The LRS was successfully used to measure the changing CSA of bovine tendons during uniaxial tensile testing. The LRS developed in this work represents a simple, quick, and accurate way of reconstructing complex cross-sectional profiles and calculating cross-sectional areas. In addition, the LRS represents the first system capable of automatically measuring changing CSA of soft tissues during tensile testing, facilitating the calculation of more accurate biomechanical properties.

  12. Resonance effects in elastic cross sections for electron scattering on pyrimidine: Experiment and theory.

    Science.gov (United States)

    Regeta, Khrystyna; Allan, Michael; Winstead, Carl; McKoy, Vincent; Mašín, Zdeněk; Gorfinkiel, Jimena D

    2016-01-14

    We measured differential cross sections for elastic (rotationally integrated) electron scattering on pyrimidine, both as a function of angle up to 180(∘) at electron energies of 1, 5, 10, and 20 eV and as a function of electron energy in the range 0.1-14 eV. The experimental results are compared to the results of the fixed-nuclei Schwinger variational and R-matrix theoretical methods, which reproduce satisfactorily the magnitudes and shapes of the experimental cross sections. The emphasis of the present work is on recording detailed excitation functions revealing resonances in the excitation process. Resonant structures are observed at 0.2, 0.7, and 4.35 eV and calculations for different symmetries confirm their assignment as the X̃(2)A2, Ã(2)B1, and B̃(2)B1 shape resonances. As a consequence of superposition of coherent resonant amplitudes with background scattering the B̃(2)B1 shape resonance appears as a peak, a dip, or a step function in the cross sections recorded as a function of energy at different scattering angles and this effect is satisfactorily reproduced by theory. The dip and peak contributions at different scattering angles partially compensate, making the resonance nearly invisible in the integral cross section. Vibrationally integrated cross sections were also measured at 1, 5, 10 and 20 eV and the question of whether the fixed-nuclei cross sections should be compared to vibrationally elastic or vibrationally integrated cross section is discussed.

  13. Use of electronic cigarettes among Romanian university students: a cross-sectional study.

    Science.gov (United States)

    Lotrean, Lucia Maria

    2015-04-11

    Because electronic cigarettes are relatively new, data on usage patterns and factors which influence them are sparse. Hence, this study aims at assessing awareness, beliefs about electronic cigarettes and experimentation with them among university students from Romania- a country where the sales and marketing of these products are widespread. Secondly, correlates of electronic cigarette experimentation will also be investigated. A cross-sectional study was performed by means of anonymous questionnaires among 480 students, aged 19-24, from Cluj-Napoca, Romania, between April-May 2013. The results show that 92.5% of the students have heard about e-cigarettes; out of these, one quarter (53.3% of the smokers, 25% of the ex-smokers, 5.5% of the non-smokers) have tried electronic cigarettes at least once during lifetime. The results of the multinomial logistic regression point out that the correlates of electronic cigarette experimentation were: male gender, being a smoker of traditional cigarettes, having friends who experimented with electronic cigarettes, having stronger beliefs that electronic cigarettes could help them quit smoking and being less convinced that they are used only by smokers. The explained variance was 59%. The results underline the importance of addressing the issue of e-cigarette use through health education programs and regulatory interventions, since e-cigarettes are a reality faced by the Romanian youth.

  14. Communicating Ebola through social media and electronic news media outlets: A cross-sectional study.

    Science.gov (United States)

    Househ, Mowafa

    2016-09-01

    Social media and electronic news media activity are an important source of information for the general public. Yet, there is a dearth of research exploring the use of Twitter and electronic news outlets during significant worldly events such as the recent Ebola Virus scare. The purpose of this article is to investigate the use of Twitter and electronic news media outlets in communicating Ebola Virus information. A cross-sectional survey of Twitter data and Google News Trend data from 30 September till 29 October, 2014 was conducted. Between 30 September and 29 October, there were approximately 26 million tweets (25,925,152) that contained the word Ebola. The highest number of correlated activity for Twitter and electronic news outlets occurred on 16 October 2014. Other important peaks in Twitter data occurred on 1 October, 6 October, 8 October, and 12 October, 2014. The main influencers of the Twitter feeds were news media outlets. The study reveals a relationship between electronic news media publishing and Twitter activity around significant events such as Ebola. Healthcare organizations should take advantage of the relationship between electronic news media and trending events on social media sites such as Twitter and should work on developing social media campaigns in co-operation with leading electronic news media outlets (e.g. CNN, Yahoo, Reuters) that can have an influence on social media activity. © The Author(s) 2015.

  15. Absolute experimental cross sections for the electron impact ionization of rubidium. Technical summary report

    International Nuclear Information System (INIS)

    Hughes, D.W.; Feeney, R.K.

    1980-01-01

    The absolute cross sections for the double, triple, and quadruple ionization of Rb + ions by electron impact have been measured from below their respective thresholds to approximately 3000 eV. This determination has been accomplished using a crossed beam facility in which monoenergetic beams of ions and electrons are caused to intersect at right angles in a well-defined collision volume. Multiply charged, product ions born as a result of the electron impact are deflected into their respective detectors by cascaded electrostatic analyzers. The multiply charged beam current component is measured by means of a vibrating reed electrometer operating in the rate-of-charge mode. The required singly charged rubidium ions are produced in a thermionic ion source and pass through a series of focusing, collimating and deflecting structures before entering the interaction region. A thermionically generated, rectangular electron beam intercepts the target ions in a spatially designated collision volume. Just prior to entering this interaction region the two beams can be made to pass through a movable slit scanner which determines their spatial profiles. The various charged particle currents, energies and beam current density distributions represent the experimental data from which the desired absolute cross sections have been determined. The results obtained with this technique are compared with available theoretical predictions of the appropriate cross sections

  16. Measurement and calculation of excitation cross sections and level ionization by electron impact

    International Nuclear Information System (INIS)

    Blanco Rames, F.

    1990-01-01

    The experimental and theoretical study of the atomic structure in neutral noble gases is studied in this work. It mainly deals with the determination of total cross sections by electron impact and transition probabilities, including: Chapter 1: Theoretical introduction and the intermediate coupling description obtained for 420 levels of s, p and d configurations in neutral noble gases. Chapter 2: Experimental and theoretical values for electron collision cross sections are obtained for several levels of He, Ne, Ar and Kr. Our results as well as those available from existing bibliography are sumarized and compared. By means of an intermediate coupling treatment a number of regularities is found that provides us with some useful approximate semi empirical expressions. Chapter 3: Determination of lifetime and transition probabilities. Lifetime measurements are carried out by means of laser excitation and multichannel delayed coincidences technique. The experimental setup and electronics are also described. Chapter 4: Details the experimental setup developed for electron cross sections measurement by the optical method. The difficulties of this method and their treatment are also shown. (Author)

  17. Production of a high-current microsecond electron beam with a large cross section

    International Nuclear Information System (INIS)

    Abdullin, E.N.; Belomytsev, S.Ya.; Bugaev, S.P.; Gorbachev, S.I.; Zaslavskii, V.M.; Zorin, V.P.; Koval'chuk, B.M.; Loginov, S.V.; Matyukov, Yu.N.; Rasputin, R.M.; Tolkachev, V.S.; Shchanin, P.M.

    1991-01-01

    Obtaining high-current wide-aperture electron beams is an important problem in the development of laser technology for controlled nuclear fusion and for solving ecological and technological problems. The main scheme for producing such beams involves the use of generators with intermediate energy storage devices and burst-emission vacuum diodes. Beam pinching is prevented by using an external magnetic field or sectioning the diode into magnetically insulated diodes with currents lower than the limiting current. The length of the electron-current pulse varies from tens to hundreds of nano-seconds and is limited by the parameters of the intermediate storage device. Here the authors study the formation of a high-current electron beam with a square cross section and a current of the order of the limiting current of the diode in the absence of an external magnetic field as well as a 'fast' storage device in the power supply circuit. These conditions as a whole correspond to a simpler electron-source circuit, but the beam forming becomes more complicated. The reason for this is that there is no external magnetic field and that the role of plasma processes in the diode is enhanced by the greater length of the electron-current pulses

  18. Effect of tissue inhomogeneity on dose distribution of point sources of low-energy electrons

    International Nuclear Information System (INIS)

    Kwok, C.S.; Bialobzyski, P.J.; Yu, S.K.; Prestwich, W.V.

    1990-01-01

    Perturbation in dose distributions of point sources of low-energy electrons at planar interfaces of cortical bone (CB) and red marrow (RM) was investigated experimentally and by Monte Carlo codes EGS and the TIGER series. Ultrathin LiF thermoluminescent dosimeters were used to measure the dose distributions of point sources of 204 Tl and 147 Pm in RM. When the point sources were at 12 mg/cm 2 from a planar interface of CB and RM equivalent plastics, dose enhancement ratios in RM averaged over the region 0--12 mg/cm 2 from the interface were measured to be 1.08±0.03 (SE) and 1.03±0.03 (SE) for 204 Tl and 147 Pm, respectively. The Monte Carlo codes predicted 1.05±0.02 and 1.01±0.02 for the two nuclides, respectively. However, EGS gave consistently 3% higher dose in the dose scoring region than the TIGER series when point sources of monoenergetic electrons up to 0.75 MeV energy were considered in the homogeneous RM situation or in the CB and RM heterogeneous situation. By means of the TIGER series, it was demonstrated that aluminum, which is normally assumed to be equivalent to CB in radiation dosimetry, leads to an overestimation of backscattering of low-energy electrons in soft tissue at a CB--soft-tissue interface by as much as a factor of 2

  19. Three-dimensional reconstruction of colorectal tumors from serial tissue sections by computer graphics: a preliminary study.

    Science.gov (United States)

    Kikuchi, S; Matsuzaki, H; Kondo, K; Ohtani, Y; Ihara, A; Hiki, Y; Kakita, A; Kuwao, S

    2000-01-01

    We present herein the three-dimensional reconstruction of colorectal tumors, with particular reference to growth pattern into each layer of the colorectal wall, and measurement of tumor volume and surface area. Conventional tissue section images of colorectal tumors were analyzed using a computer graphics analysis program. The two-dimensional extent of invasion by each tumor into each layer of intestinal wall were determined from the images of each section. Based on data from multiple sections, tumor and surrounding normal tissue layers were reconstructed three-dimensionally, and volume and surface area of the tumors were determined. Using this technique, three-dimensional morphology of tumor and tumor progression into colorectal wall could be determined. Volume and surface area of the colon tumor were 4871 mm3 and 1741 mm2, respectively. Volume and surface area of the rectal tumor were 1090 mm3 and 877 mm2, respectively. This technique may provide a new approach for pathological analysis of colorectal carcinoma.

  20. METHOD FOR OBSERVATION OF DEEMBEDDED SECTIONS OF FISH GONAD BY SCANNING ELECTRON MICROSCOPY

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This article reports a method for examining the intracellular structure of fish gonads using a scanning electron microscope(SEM). The specimen preparation procedure is similar to that for transmission electron microscopy wherein samples cut into semi-thin sections are fixed and embedded in plastic. The embedment matrix was removed by solvents. Risen-free specimens could be observed by SEM. The morphology of matured sperms in the gonad was very clear, and the oocyte internal structures appeared in three-dimensional images. Spheroidal nucleoli and yolk vesicles and several bundles of filaments adhered on the nucleoli could be viewed by SEM for the first time.

  1. Determining of electron temperature profile on the cross section of a Tokamak, using ECE technique

    Directory of Open Access Journals (Sweden)

    M. Hosseinpour

    2007-06-01

    Full Text Available  In this paper we have used plasma electron cyclotron emissions at the second harmonic frequency of extraordinary mode to determine the temperature profile of the plasma produced in IR-T1 Tokamak. The emissions obtained at different frequencies by a 5-channel heterodyne receiver, have been analyzed to determine the spatial variation of the electron temperature on the plasma cross section. The results have been also used to show the three-dimensional time evolution of the temperature profile during the period of confinement.

  2. Optical excitation cross-sections for electron collisions with atoms and molecules

    International Nuclear Information System (INIS)

    McConkey, J.W.; Univ. of Windsor, Ontario)

    1983-01-01

    A brief review of the status of absolute electron-impact excitation cross-section measurements for atoms and molecules is presented. Some of the reasons for the wide discrepancies which exist in the published data are discussed. Tables are presented of recent publications in the field which are not included in the J.I.L.A. compilations. A tabular compilation of the existing data for e-impact on H 2 O is also given and discussed. Some recent experiments of particular interest to the development of the theory of electron-molecule excitation are mentioned. 112 references, 3 figures, 3 tables

  3. Differential cross sections for inelastic scattering of electrons on Kr and Xe atoms at intermediate energies

    International Nuclear Information System (INIS)

    Filipovic, D.M.

    1989-01-01

    Electron-impact excitation of the larger- number noble-gas atoms is a way of understanding excitation mechanisms in atomic collisional processes. Krypton and xenon have the largest atomic number of all the stable noble gases. Therefore, effects dependent on the size of a target atom, such as alignment and orientation of the atomic outer shell charge cloud after collisional excitation, are best observed by studying these atoms. Normalized, absolute differential cross sections (DCS's) for the lowest electronic states of Kr and Xe atoms, at intermediate energies, are the subject of this report

  4. Calculation of total cross sections for electron and positron scattering on sodium and potassium

    International Nuclear Information System (INIS)

    McCarthy, I.E.; Ratnavelu, K.; Zhou, Y.

    1993-02-01

    Total cross sections for electron and positron scattering on sodium and potassium are calculated at various energies and compared with experiment. The method use is the coupled-channels-optical method with the equivalent-local polarisation potential, which takes all channels into account. For electrons the calculations are checked by comparison with coupled-channels-optical calculations using a detailed polarisation potential that makes only one approximation, that of weak coupling in the ionisation space. The polarisation potential for positrons includes effects of ionisation and positronium formation. 13 refs., 2 tabs

  5. Detection of free radicals in γ-irradiated seasnail hard tissues by electron paramagnetic resonance

    International Nuclear Information System (INIS)

    Koeseoglu, Rahmi; Koeksal, Fevzi

    2003-01-01

    Gamma-irradiated seasnail (from family of Helix lukortium) hard tissues (CaCO 3 ) were investigated by electron paramagnetic resonance (EPR) at room temperature. The radicals produced by γ-irradiation in seasnail were attributed to orthorhombic C · O 2 - , freely rotating C · O 2 - , orthorhombic C · O 3 - , axial C · O 3 - , and axial C · O 3 3- free radicals. Unirradiated seasnail hard tissues also feature Mn 2+ ions in their EPR spectra. The hyperfine values were determined for the 13 C nucleus in the orthorhombic C · O 2 - and axial C · O 3 3- free radicals and for the manganese impurity ions. The g values of all the free radicals have been measured. The results were compared with the literature data for similar defects

  6. Gas electron multiplier (GEM) operation with tissue-equivalent gases at various pressures

    International Nuclear Information System (INIS)

    Farahmand, M.; Bos, A.J.J.; Eijk, C.W.E. van

    2003-01-01

    We have studied the operation of two different Gas Electron Multiplier (GEM) structures in both methane and propane based Tissue-Equivalent (TE) gases at different pressures varying from 0.1 to 1 atm. This work was motivated to explore the possibility of using a GEM for a new type of Tissue Equivalent Proportional Counter. In methane based TE gas, a maximum safe GEM gain of 1.5x10 3 has been reached while in propane based TE gas this is 6x10 3 . These maxima have been reached at different gas pressures depending on GEM structure and TE gas. Furthermore, we observed a decrease of the GEM gain in time before it becomes stable. Charge up/polarisation effects can explain this

  7. Separating spectral mixtures in hyperspectral image data using independent component analysis: validation with oral cancer tissue sections

    Science.gov (United States)

    Duann, Jeng-Ren; Jan, Chia-Ing; Ou-Yang, Mang; Lin, Chia-Yi; Mo, Jen-Feng; Lin, Yung-Jiun; Tsai, Ming-Hsui; Chiou, Jin-Chern

    2013-12-01

    Recently, hyperspectral imaging (HSI) systems, which can provide 100 or more wavelengths of emission autofluorescence measures, have been used to delineate more complete spectral patterns associated with certain molecules relevant to cancerization. Such a spectral fingerprint may reliably correspond to a certain type of molecule and thus can be treated as a biomarker for the presence of that molecule. However, the outcomes of HSI systems can be a complex mixture of characteristic spectra of a variety of molecules as well as optical interferences due to reflection, scattering, and refraction. As a result, the mixed nature of raw HSI data might obscure the extraction of consistent spectral fingerprints. Here we present the extraction of the characteristic spectra associated with keratinized tissues from the HSI data of tissue sections from 30 oral cancer patients (31 tissue samples in total), excited at two different wavelength ranges (330 to 385 and 470 to 490 nm), using independent and principal component analysis (ICA and PCA) methods. The results showed that for both excitation wavelength ranges, ICA was able to resolve much more reliable spectral fingerprints associated with the keratinized tissues for all the oral cancer tissue sections with significantly higher mean correlation coefficients as compared to PCA (p<0.001).

  8. Effective melanin depigmentation of human and murine ocular tissues: an improved method for paraffin and frozen sections.

    Directory of Open Access Journals (Sweden)

    Caroline Manicam

    Full Text Available PURPOSE: The removal of excessive melanin pigments that obscure ocular tissue morphology is important to address scientific questions and for differential diagnosis of ocular tumours based on histology. Thus, the goal of the present study was to establish an effective and fast melanin bleaching method for paraffin and frozen mouse and human ocular tissues. METHODS: Paraffin-embedded and frozen ocular specimens from mice and human donors were subjected to bleaching employing two methods. The first employed potassium permanganate (KMnO4 with oxalic acid, and the second 10% hydrogen peroxide (H2O2. To determine optimal bleaching conditions, depigmentation was carried out at various incubation times. The effect of diluents used for 10% H2O2 was assessed using phosphate-buffered saline (PBS, and deionized water. Three different slide types and two fixatives, which were ice-cold acetone with 80% methanol, and 4% paraformaldehyde (PFA were used to determine the optimal conditions for better tissue adherence during bleaching. All tissues were stained in hematoxylin and eosin for histological evaluation. RESULTS: Optimal bleaching was achieved using warm 10% H2O2 diluted in PBS at 65°C for 120 minutes. Chromium-gelatin-coated slides prevented tissue detachment. Adherence of cryosections was also improved with post-fixation using 4% PFA and overnight air-drying at RT after cryosectioning. Tissue morphology was preserved under these conditions. Conversely, tissues bleached in KMnO4/oxalic acid demonstrated poor depigmentation with extensive tissue damage. CONCLUSIONS: Warm dilute H2O2 at 65°C for 120 minutes rapidly and effectively bleached both cryo- and paraffin sections of murine and human ocular tissues.

  9. Effective melanin depigmentation of human and murine ocular tissues: an improved method for paraffin and frozen sections.

    Science.gov (United States)

    Manicam, Caroline; Pitz, Susanne; Brochhausen, Christoph; Grus, Franz H; Pfeiffer, Norbert; Gericke, Adrian

    2014-01-01

    The removal of excessive melanin pigments that obscure ocular tissue morphology is important to address scientific questions and for differential diagnosis of ocular tumours based on histology. Thus, the goal of the present study was to establish an effective and fast melanin bleaching method for paraffin and frozen mouse and human ocular tissues. Paraffin-embedded and frozen ocular specimens from mice and human donors were subjected to bleaching employing two methods. The first employed potassium permanganate (KMnO4) with oxalic acid, and the second 10% hydrogen peroxide (H2O2). To determine optimal bleaching conditions, depigmentation was carried out at various incubation times. The effect of diluents used for 10% H2O2 was assessed using phosphate-buffered saline (PBS), and deionized water. Three different slide types and two fixatives, which were ice-cold acetone with 80% methanol, and 4% paraformaldehyde (PFA) were used to determine the optimal conditions for better tissue adherence during bleaching. All tissues were stained in hematoxylin and eosin for histological evaluation. Optimal bleaching was achieved using warm 10% H2O2 diluted in PBS at 65°C for 120 minutes. Chromium-gelatin-coated slides prevented tissue detachment. Adherence of cryosections was also improved with post-fixation using 4% PFA and overnight air-drying at RT after cryosectioning. Tissue morphology was preserved under these conditions. Conversely, tissues bleached in KMnO4/oxalic acid demonstrated poor depigmentation with extensive tissue damage. Warm dilute H2O2 at 65°C for 120 minutes rapidly and effectively bleached both cryo- and paraffin sections of murine and human ocular tissues.

  10. The feasibility of using neural networks to obtain cross sections from electron swarm data

    International Nuclear Information System (INIS)

    Morgan, W.L.

    1991-01-01

    This paper reports that although still more a curiosity than an accepted technique in computational modeling, the very new field of neural computing is beginning to find applications in physics. Presented in some background on neural computing and a discussion on the use of neural networks to obtain electron-impact cross sections from measured drift velocities, characteristic energies, and other swarm data. This is what is known as an inverse problem, a class of problems for which neural networks may be frequently superior to other numerical algorithms. Momentum transfer cross sections obtained for a model problem and for xenon using a neural network are presented

  11. Differential cross sections for the elastic scattering of intermediate energy electrons from sodium

    International Nuclear Information System (INIS)

    Teubner, P.J.O.; Buckner, S.J.; Noble, C.J.

    1977-11-01

    Differential cross sections for the elastic scattering of electrons from sodium have been measured with high angular resolution for incident energies of 54.4, 75, 100 and 150 eV and over an angular range of 12 0 to 140 0 . The experimental data are compared with calculations based on the First Born approximation, the Glauber approximation and a close coupling impact parameter calculation. Calculations have been carried out for an optical model using the prescription of Vanderpoorten for localizing the absorptive part of the potential. Of the theoretical calculations the optical model is found to best reproduce the general features of the cross section at all energies. (Author)

  12. Empirical Fit to Precision Inclusive Electron-Proton Cross Sections in the Resonance Region

    International Nuclear Information System (INIS)

    M.E. Christy; Peter Bosted

    2007-01-01

    An empirical fit is described to measurements of inclusive inelastic electron-proton cross sections in the kinematic range of four-momentum transfer 0 (le) Q 2 2 and final state invariant mass 1.1 2 ∼ 7.5 GeV 2 , and photoproduction data at Q 2 = 0. Compared to previous fits, the present fit covers a wider kinematic range, fits both transverse and longitudinal cross sections, and features smooth transitions to the photoproduction data at Q 2 =0 and DIS data at high Q 2 and W

  13. MUTIL, Asymmetry Factor of Mott Cross-Sections for Electron, Positron Scattering

    International Nuclear Information System (INIS)

    Idoeta, R.; Legarda, F.

    2002-01-01

    1 - Description of program or function: The asymmetry factor S of Mott's differential cross section for the scattering of electrons and positrons by point nuclei without screening is calculated for any energy, atomic number and angle of scattering. 2 - Method of solution: We have summed the conditionally convergent series, F and G, appearing in the asymmetry factor using two consecutive transformations: The one of Yennie, Ravenhall and Wilson and that of Euler till we have seven times six significant figures repeated in the factor S. 3 - Restrictions on the complexity of the problem: Those appearing in the use of Mott's cross section for unscreened point nuclei

  14. Cross sections for electron-impact excitation of argon by fourier transform spectroscopy

    International Nuclear Information System (INIS)

    Chilton, J.E.; Boffard, J.B.; Chun C.L.

    1996-01-01

    The authors report absolute measurements of electron-impact excitation cross sections out of the ground level to the ten levels in the 3p 5 4p configuration of argon. The apparent excitation cross sections are determined by measuring the optical cross sections for the emission lines in the 3p 5 4p → 3p 5 4s manifold. For cascade corrections the authors measured the optical cross sections for the various 3p 5 5s → 3p 5 4p and 3p 5 4p infrared lines using a Fourier transform weak emission spectrometer to obtain the direct excitation cross sections from the optical data. Although the optical cross sections vary with pressure in the regime of 0.1 to 6 mTorr, the direct cross sections remain invariant. These pressure effects are understood within the framework of a radiation-reabsorption model. The excitation functions for the different transitions are also found to show considerable variation in shape

  15. Calculation of the differential cross sections of excitation and ionization of a helium atom by electrons

    International Nuclear Information System (INIS)

    Demkin, V.P.; Pecheritsyn, A.A.

    1995-01-01

    Equations for the amplitudes and differential cross sections of electronic excitation and ionization of a helium atom are derived in the approximation of a open-quotes frozenclose quotes ion core. The wave functions of the discrete states are chosen in the form of generalized hydrogenlike orbitals. The radial wave functions of the continuous spectrum are determined by solving the equation of motion numerically. The differential excitation cross sections of excitation of the 2p, 3p, and 4p levels and ionization of a helium atom by electrons are calculated in the energy range up to 50 eV. Estimates are obtained for the nonorthogonal wave functions in the amplitudes of the excitation and ionization processes. It is shown that the given method is more compatible with experiment than the Born method

  16. Integrated cross sections for the ionisation of atomic hydrogen by electron impact

    International Nuclear Information System (INIS)

    Konovalov, D.A.; McCarthy, I.E.

    1992-05-01

    Distorted-wave Born approximation (DWBA) calculations are reported for singly-differential and total cross sections for the electron impact ionisation for atomic hydrogen at 25, 40, 60, 100, 150 and 250 eV. The theory is compared with available experiments. At all the energies except 25 eV the theory predicts a lower singly-differential cross section for the low-energy side of the secondary-electron energies (<5 eV), compared to the only available absolute measurements of Shyn (1992). The DWBA calculation is in good agreement with the experiment at 25 eV but only if e-e post-collision interaction is included in the theory in some way. 23 refs., 2 figs

  17. Singly differential electron emission cross sections for ionization of helium by protons

    International Nuclear Information System (INIS)

    Barna, I.F.; Gagyi-Palffy, A.C.; Gulyas, L.; Tokesi, K.; Burgdoerfer, J.

    2005-01-01

    Angular differential cross sections are calculated for electrons emitted in proton-helium collisions within the framework of the time-dependent coupled channel-method. The channel wave functions are constructed from Slater functions and Coulomb wave packets. As projectiles we consider protons with energies between 0.3 and 1.5 MeV. We compare our results with experimental data and other theoretical calculations using the first Born approximation, different distorted wave models and classical trajectory Monte Carlo simulations

  18. Associations of attitudes towards electronic cigarettes with advertisement exposure and social determinants: a cross sectional study

    OpenAIRE

    Reinhold, Benjamin; Fischbein, Rebecca; Bhamidipalli, Surya Sruthi; Bryant, Jennifer; Kenne, Deric R.

    2017-01-01

    Background The exposure of young adults to electronic cigarette (e-cigarette) advertisements has risen rapidly. E-cigarette ads have been shown to increase short term perceived acceptability of using e-cigarettes in places where traditional cigarettes are banned. We set out to investigate if advertising exposure was related to perceptions of harm, addictiveness, and acceptability of use of e-cigarettes in places where traditional cigarettes are banned. Methods Using a cross-sectional design, ...

  19. Partial and total electronic stopping cross sections of atoms and solids for protons

    International Nuclear Information System (INIS)

    Kaneko, Toshiaki.

    1990-12-01

    Based on a wave packet theory (Phys. Rev. A40, 2188(1989); Phys. Stat. Sol. (B)156,49(1989)), partial and total electronic cross sections of target elements in atomic and solid phases with atomic number Z ranging from 2 (He) to 92 (U) are tabulated shell by shell for protons with velocity v from 0.2V 0 to 2OV 0 (V 0 =2.18 x 10 8 cm/s). (author)

  20. Electron microscopy of rapid identification of animal viruses in hematoxylin-eosin sections.

    Science.gov (United States)

    Bhatnagar, R; Johnson, G R; Christian, R G

    1977-01-01

    Routine hematoxylin-eosin stained, paraffin sections were processed for electron microscopy, using a rapid method for localization of animal viruses. Formalin fixation was effective in preserving DNA as well as RNA viruses, however cellular fine structural details and organelles were not well preserved. The procedure is useful for morphological recognition of viral groups and as a rapid diagnostic aid for identifying viral disease. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 5. Fig. 6. PMID:72592

  1. A critical comparison of electron scattering cross sections measured by single collision and swarm techniques

    International Nuclear Information System (INIS)

    Buckman, S.J.; Brunger, M.J.

    1996-07-01

    Electron scattering cross sections (elastic, rotational and vibrational excitation) for a number of atomic and (relatively) single molecular systems are examined. Particular reference is made to the level of agreement which is obtained from the application of the completely different measurement philosophies embodied in 'beam' and 'swarm' techniques. The range of energies considered is generally restricted to the region below 5 eV. 142 refs., 1 tab., 12 figs

  2. Absolute 3PO triple differential cross sections for electron-helium Wannier threshold ionisation

    International Nuclear Information System (INIS)

    Carruthers, D.R.J.; Crothers, D.S.F.

    1992-01-01

    The uniform semiclassical approximation of Crothers is employed to find a final-state 3 P O wave function for threshold electron impact ionisation of helium. The Kohn variational principle is applied perturbatively to evaluate the scattering amplitude and hence the absolute 3 P O contribution to the triple differential cross sections. These plus the previous absolute singlet results of Crothers are compared favourably with the relative experimental results of Selles et al. (orig.)

  3. Experimental cross sections for the pickup of electrons by relativistic nuclei

    International Nuclear Information System (INIS)

    Raisbeck, G.M.; Crawford, H.J.; Lindstroem, P.J.; Greiner, D.E.; Beiser, F.S.; Heckman, H.H.

    1977-01-01

    Cross sections for the pickup of electrons in a variety of targets have been measured with beams of 12 C (150, 250, 400 MeV/n), 20 Ne (250, 400, 1050, 2100 MeV/n) and 40 Ar (400, 1050 MeV/n) from the Bevalac accelerator. The interpretation of the results in terms of radiative and non-radiative capture, and the implications for cosmic ray propagation studies are discussed. (author)

  4. Comparison of inelastic electron and positron scattering cross sections on 12C and 27Al

    International Nuclear Information System (INIS)

    Hartwig, S.; Heimlich, F.H.; Huber, G.; Roessle, E.; Koebberling, M.; Moritz, J.; Schmidt, K.H.; Wegener, D.; Zeller, D.; Bleckwenn, J.

    1977-06-01

    The +/- ratio R of the cross sections for inelastic positron and electron scattering on 12 C and 27 Al has been measured for four momentum transfers (0.08 - 0.45) GeV 2 /c 2 of the virtual photon and invariant masses 0.95 GeV +- 0.0007), no q 2 respectively W dependence of the ratio is observed. (orig.) [de

  5. Tissue

    Directory of Open Access Journals (Sweden)

    David Morrissey

    2012-01-01

    Full Text Available Purpose. In vivo gene therapy directed at tissues of mesenchymal origin could potentially augment healing. We aimed to assess the duration and magnitude of transene expression in vivo in mice and ex vivo in human tissues. Methods. Using bioluminescence imaging, plasmid and adenoviral vector-based transgene expression in murine quadriceps in vivo was examined. Temporal control was assessed using a doxycycline-inducible system. An ex vivo model was developed and optimised using murine tissue, and applied in ex vivo human tissue. Results. In vivo plasmid-based transgene expression did not silence in murine muscle, unlike in liver. Although maximum luciferase expression was higher in muscle with adenoviral delivery compared with plasmid, expression reduced over time. The inducible promoter cassette successfully regulated gene expression with maximum levels a factor of 11 greater than baseline. Expression was re-induced to a similar level on a temporal basis. Luciferase expression was readily detected ex vivo in human muscle and tendon. Conclusions. Plasmid constructs resulted in long-term in vivo gene expression in skeletal muscle, in a controllable fashion utilising an inducible promoter in combination with oral agents. Successful plasmid gene transfection in human ex vivo mesenchymal tissue was demonstrated for the first time.

  6. Electron excitation cross sections of the 2p53s levels of neon

    International Nuclear Information System (INIS)

    Phillips, M.H.

    1982-01-01

    The electron excitation cross sections of the four 2p 5 3s levels of neon are measured by means of a new technique using laser induced fluorescenc. The values of both the apparent and direct excitatiuon cross sections are given as a function of incident electron energy (0-100eV for 1s 3 and 1s 5 metastable levels and 0-300 eV for the 1s 2 and 1s 4 resonance levels). The metastable levels are triplet states and their cross sections are sharply peaked. At the peak, 30 eV, the measured values of the cross sections are 0.59 x 10 -18 cm 2 for the 1s 3 level and 3.2 x 10 -18 cm 2 for the 1s 5 level. The 1s 2 and 1s 4 levels are mixtures of singlet and triplet states, and the cross sections are broad and peak at 60 eV. The apparent cross sections at 60 eV are 13.5 x 10 -18 cm 2 for the 1s 2 level and 2.9 x 10 -18 cm 2 for the 1s 4 level. Measurement of the atomic number density of each of the 1s levels is performed using the pulsed laser induced fluorescence. The measured values of the number density and the excitation cross section are used to obtain the lifetime of each level. The measured value of the reciprocal of the 1s 3 and 1s 5 lifetime is 1 x 10 5 s -1 . The 1s 2 and 1s 4 levels are radiation trapped, and the reciprocals of their lifetimes are 4 x 10 5 s -1 and 3 x 10 5 s -1 respectively. The electron excitation cross sections of the magnetic sublevels of each 1s level are obtained by measuring the polarization of the laser induced fluorescence as a function of laser polarization. The excitation cross sections of each of the magnetic sublevels within a particular 1s level are equal within experimental uncertainty

  7. Differential cross sections and cross-section ratios for the electron-impact excitation of the neon 2p53s configuration

    International Nuclear Information System (INIS)

    Khakoo, M. A.; Wrkich, J.; Larsen, M.; Kleiban, G.; Kanik, I.; Trajmar, S.; Brunger, M.J.; Teubner, P.J.O.; Crowe, A.; Fontes, C.J.; Clark, R.E.H.; Zeman, V.; Bartschat, K.; Madison, D.H.; Srivastava, R.; Stauffer, A.D.

    2002-01-01

    Electron-impact differential cross-section measurements for the excitation of the 2p 5 3s configuration of Ne are reported. The Ne cross sections are obtained using experimental differential cross sections for the electron-impact excitation of the n=2 levels of atomic hydrogen [Khakoo et al., Phys. Rev. A 61, 012701-1 (1999)], and existing experimental helium differential cross-section measurements, as calibration standards. These calibration measurements were made using the method of gas mixtures (Ne and H followed by Ne and He), in which the gas beam profiles of the mixed gases are found to be the same within our experimental errors. We also present results from calculations of these differential cross sections using the R-matrix and unitarized first-order many-body theory, the distorted-wave Born approximation, and relativistic distorted-wave methods. Comparison with available experimental differential cross sections and differential cross-section ratios is also presented

  8. Cross-section measurements for the fragmentation of CHClF{sub 2} by electron impact

    Energy Technology Data Exchange (ETDEWEB)

    Sigaud, L; Ferreira, Natalia; Wolff, W; Santos, A C F dos; Montenegro, E C [Instituto de FIsica, Universidade Federal do Rio de Janeiro, PO 68528, 21941-972 Rio de Janeiro, RJ (Brazil); De Jesus, V L B; Menezes, R S [Instituto Federal de Educacao, Ciencia e Tecnologia do Rio de Janeiro (IFRJ), Campus Nilopolis, R. Lucio Tavares 1045, 26530-060 Nilopolis, RJ (Brazil); De Barros, A L F [CEFET/RJ, Av. Maracana 229, 20271-110 Rio de Janeiro, RJ (Brazil); Rocha, A B [Instituto de QuImica, Universidade Federal do Rio de Janeiro, Cidade Universitaria-Ilha do Fundao, 21941-614 Rio de Janeiro, RJ (Brazil); Shah, M B, E-mail: lucas@if.ufrj.b [School of Maths and Physics, The Queen' s University of Belfast, University Road. Belfast, BT7 1NN (United Kingdom)

    2010-05-28

    CFC compounds present in the upper atmosphere have a significant effect on the environment, strongly contributing to the increase of the hole in the ozone layer. Recent studies show that low-energy electron impact is an important process in the dissociation of these molecules, creating atomic chlorine, which breaks down ozone molecules. In this work, the CHClF{sub 2} fragmentation by electron impact in the 40-400 eV energy range is measured. Total and partial cross sections have been obtained, showing the predominance of the release of neutral chlorine, which amounts to around 60% of the total yield. There is a strong indication that this chlorine is being released as a result of the ionization of electrons from both chlorine and fluorine orbitals.

  9. Experimental and theoretical double differential cross sections for electron impact ionization of methane

    Energy Technology Data Exchange (ETDEWEB)

    Yavuz, Murat; Ozer, Zehra Nur, E-mail: zehraerengil@aku.edu.tr; Ulu, Melike; Dogan, Mevlut [e-COL Laboratory, Department of Physics, Afyon Kocatepe University, Afyonkarahisar 03200 (Turkey); Champion, Christophe [Centre d’Etudes Nucléaires de Bordeaux Gradignan, CNRS/IN2P3, Université de Bordeaux, Gradignan 33170 (France)

    2016-04-28

    Experimental and theoretical double differential cross sections (DDCSs) for electron-induced ionization of methane (CH{sub 4}) are here reported for primary energies ranging from 50 eV to 350 eV and ejection angles between 25° and 130°. Experimental DDCSs are compared with theoretical predictions performed within the first Born approximation Coulomb wave. In this model, the initial molecular state is described by using single center wave functions, the incident (scattered) electron being described by a plane wave, while a Coulomb wave function is used for modeling the secondary ejected electron. A fairly good agreement may be observed between theory and experiment with nevertheless an expected systematic overestimation of the theory at low-ejection energies (<50 eV).

  10. Triple differential cross section for the ionization of helium by electronic impact

    Energy Technology Data Exchange (ETDEWEB)

    Diallo, Saidou, E-mail: saidou40@yahoo.fr [Laboratoire de Physique des Plasmas et de Recherches Interdisciplinaires, Universite Cheikh Anta Diop, Faculte des Sciences et Techniques, Departement de Physique, BP: 5005 Dakar-Fann (Senegal); Faye, I.G.; Diedhiou, I.A.; Tall, M.S.; Gomis, L.; Diatta, C.S. [Laboratoire de Physique des Plasmas et de Recherches Interdisciplinaires, Universite Cheikh Anta Diop, Faculte des Sciences et Techniques, Departement de Physique, BP: 5005 Dakar-Fann (Senegal)

    2011-12-01

    We report results of analytical triple differential cross sections (TDCS) for the single ionization of the helium iso-electronic ions by the electron impact. A two variational parameters wave function is used to evaluate the TDCS. This study shows the accuracy of the TDCS for helium atom and helium like ions in the first Born approximation (FBA) at high incident energy domain. The theory is quite acceptable as a fast calculation of the triple differential cross section, particularly at high energies where other theories and methods are cumbersome. A comparison is made of our calculations with previous results of the other theoretical methods and experiment. The FBA results obtained here with the two variational parameters wave function are in good agreement with the experiment data at high incident energy. The results show that the electron correlation effects are important around the maxima and influence only the extrema magnitude but not their positions. The calculations presented here are extanded to the cases where the energies of the outgoing electrons are more equal.

  11. 0.1–2000 eV electron impact cross sections for dichlorine monoxide

    Energy Technology Data Exchange (ETDEWEB)

    Goswami, Biplab; Gupta, Dhanoj; Antony, Bobby, E-mail: bka.ism@gmail.com

    2014-03-01

    Highlights: • Quantum mechanical models were used to find TCS for e-Cl{sub 2}O from 0.1 to 2000 eV. • R-matrix method at low energies (<13 eV) and SCOP at high energies (13–2000 eV). • Besides TCS, DCS, excitation cross section and momentum transfer CS also predicted. • R-matrix method identifies resonances with a possibility of DEA formation. • Resonance detected at 1.88 eV is associated with Cl{sub 2}O{sup −} anion formation. - Abstract: Scattering dynamics of dichlorine monoxide (Cl{sub 2}O) molecule by electron impact is investigated as a function of electron energy and scattering angle. Electron impact total cross sections for Cl{sub 2}O over an extensive range of impact energies from 0.1 to 2000 eV are reported in this article. Below the ionization threshold of the target, the ab initio R-matrix method and above this incident energy spherical complex optical potential formalism are used for cross section calculation. The total cross section obtained from both theories merges smoothly at the overlapping energy. The resonances obtained using DZP basis sets are located at 1.883, 3.592, 5.205 and 7.326, 8.206, 8.301, 8.452, 9.369 eV and that with 6-31G* basis sets are identified at 1.944, 3.566, 5.183, 5.261, 5.658, 8.738 and 9.187 eV with the possibility of negative ions formation. This is the first attempt to calculate the differential, and momentum transfer cross sections for Cl{sub 2}O molecule.

  12. Study of electron densities of normal and neoplastic human breast tissues by Compton scattering using synchrotron radiation

    Energy Technology Data Exchange (ETDEWEB)

    Antoniassi, M.; Conceicao, A.L.C. [Departamento de Fisica-Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto-Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo (Brazil); Poletti, M.E., E-mail: poletti@ffclrp.usp.br [Departamento de Fisica-Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto-Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo (Brazil)

    2012-07-15

    Electron densities of 33 samples of normal (adipose and fibroglangular) and neoplastic (benign and malignant) human breast tissues were determined through Compton scattering data using a monochromatic synchrotron radiation source and an energy dispersive detector. The area of Compton peaks was used to determine the electron densities of the samples. Adipose tissue exhibits the lowest values of electron density whereas malignant tissue the highest. The relationship with their histology was discussed. Comparison with previous results showed differences smaller than 4%. - Highlights: Black-Right-Pointing-Pointer Electron density of normal and neoplastic breast tissues was measured using Compton scattering. Black-Right-Pointing-Pointer Monochromatic synchrotron radiation was used to obtain the Compton scattering data. Black-Right-Pointing-Pointer The area of Compton peaks was used to determine the electron densities of samples. Black-Right-Pointing-Pointer Adipose tissue shows the lowest electron density values whereas the malignant tissue the highest. Black-Right-Pointing-Pointer Comparison with previous results showed differences smaller than 4%.

  13. Boltzmann equation analysis of electron-molecule collision cross sections in water vapor and ammonia

    International Nuclear Information System (INIS)

    Yousfi, M.; Benabdessadok, M.D.

    1996-01-01

    Sets of electron-molecule collision cross sections for H 2 O and NH 3 have been determined from a classical technique of electron swarm parameter unfolding. This deconvolution method is based on a simplex algorithm using a powerful multiterm Boltzmann equation analysis established in the framework of the classical hydrodynamic approximation. It is well adapted for the simulation of the different classes of swarm experiments (i.e., time resolved, time of flight, and steady state experiments). The sets of collision cross sections that exist in the literature are reviewed and analyzed. Fitted sets of cross sections are determined for H 2 O and NH 3 which exhibit features characteristic of polar molecules such as high rotational excitation collision cross sections. The hydrodynamic swarm parameters (i.e., drift velocity, longitudinal and transverse diffusion coefficients, ionization and attachment coefficients) calculated from the fitted sets are in excellent agreement with the measured ones. These sets are finally used to calculate the transport and reaction coefficients needed for discharge modeling in two cases of typical gas mixtures for which experimental swarm data are very sparse or nonexistent (i.e., flue gas mixtures and gas mixtures for rf plasma surface treatment). copyright 1996 American Institute of Physics

  14. Evaluation of computational models and cross sections used by MCNP6 for simulation of electron backscattering

    Energy Technology Data Exchange (ETDEWEB)

    Poškus, Andrius, E-mail: andrius.poskus@ff.vu.lt

    2016-02-01

    This work evaluates the accuracy of the single-event (SE) and condensed-history (CH) models of electron transport in Monte Carlo simulations of electron backscattering from thick layers of Be, C, Al, Cu, Ag, Au and U at incident electron energies from 200 eV to 15 MeV. The CH method is used in simulations performed with MCNP6.1, and the SE method is used in simulations performed with an open-source single-event code MCNelectron written by the author of this paper. Both MCNP6.1 and MCNelectron use mainly ENDF/B-VI.8 library data, but MCNelectron allows replacing cross sections of certain types of interactions by alternative datasets from other sources. The SE method is evaluated both using only ENDF/B-VI.8 cross sections (the “SE-ENDF/B method”, which is equivalent to using MCNP6.1 in SE mode) and with an alternative set of elastic scattering cross sections obtained from relativistic (Dirac) partial-wave (DPW) calculations (the “SE-DPW method”). It is shown that at energies from 200 eV to 300 keV the estimates of the backscattering coefficients obtained using the SE-DPW method are typically within 10% of the experimental data, which is approximately the same accuracy that is achieved using MCNP6.1 in CH mode. At energies below 1 keV and above 300 keV, the SE-DPW method is much more accurate than the SE-ENDF/B method due to lack of angular distribution data in the ENDF/B library in those energy ranges. At energies from 500 keV to 15 MeV, the CH approximation is roughly twice more accurate than the SE-DPW method, with the average relative errors equal 7% and 14%, respectively. The energy probability density functions (PDFs) of backscattered electrons for Al and Cu, calculated using the SE method with DPW cross sections when energy of incident electrons is 20 keV, have an average absolute error as low as 4% of the average PDF. This error is approximately twice less than the error of the corresponding PDF calculated using the CH approximation. It is concluded

  15. Absolute total and partial dissociative cross sections of pyrimidine at electron and proton intermediate impact velocities

    Energy Technology Data Exchange (ETDEWEB)

    Wolff, Wania, E-mail: wania@if.ufrj.br; Luna, Hugo; Sigaud, Lucas; Montenegro, Eduardo C. [Instituto de Física, Universidade Federal do Rio de Janeiro, PO 68528, 21941-972 Rio de Janeiro, RJ (Brazil); Tavares, Andre C. [Departamento de Física, Pontificia Universidade Católica do Rio de Janeiro, PO 38071, Rua Marquês de São Vicente 225, 22453-900 Rio de Janeiro, RJ (Brazil)

    2014-02-14

    Absolute total non-dissociative and partial dissociative cross sections of pyrimidine were measured for electron impact energies ranging from 70 to 400 eV and for proton impact energies from 125 up to 2500 keV. MOs ionization induced by coulomb interaction were studied by measuring both ionization and partial dissociative cross sections through time of flight mass spectrometry and by obtaining the branching ratios for fragment formation via a model calculation based on the Born approximation. The partial yields and the absolute cross sections measured as a function of the energy combined with the model calculation proved to be a useful tool to determine the vacancy population of the valence MOs from which several sets of fragment ions are produced. It was also a key point to distinguish the dissociation regimes induced by both particles. A comparison with previous experimental results is also presented.

  16. Temperature dependence of the cross section for the fragmentation of thymine via dissociative electron attachment

    Energy Technology Data Exchange (ETDEWEB)

    Kopyra, Janina [Faculty of Science, Siedlce University, 3 Maja 54, 08-110 Siedlce (Poland); Abdoul-Carime, Hassan, E-mail: hcarime@ipnl.in2p3.fr [Université de Lyon, Université Claude Bernard Lyon1, Institut de Physique Nucléaire de Lyon, CNRS/IN2P3 UMR 5822, 43 Bd du 11 novembre 1918, 69622 Villeurbanne Cedex (France)

    2015-05-07

    Providing experimental values for absolute Dissociative Electron Attachment (DEA) cross sections for nucleobases at realistic biological conditions is a considerable challenge. In this work, we provide the temperature dependence of the cross section, σ, of the dehydrogenated thymine anion (T − H){sup −} produced via DEA. Within the 393-443 K temperature range, it is observed that σ varies by one order of magnitude. By extrapolating to a temperature of 313 K, the relative DEA cross section for the production of the dehydrogenated thymine anion at an incident energy of 1 eV decreases by 2 orders of magnitude and the absolute value reaches approximately 6 × 10{sup −19} cm{sup 2}. These quantitative measurements provide a benchmark for theoretical prediction and also a contribution to a more accurate description of the effects of ionizing radiation on molecular medium.

  17. Total cross section for chloroflouromethanes and CClx radicals by electron impact

    International Nuclear Information System (INIS)

    Gupta, Dhanoj; Antony, Bobby

    2013-01-01

    Highlights: ► A model to find total CS for freons and CCl x by e − impact is employed. ► Complex optical potential formalism is used to find total inelastic and elastic CS. ► Result shows very good agreement with previous data wherever available. ► Maiden attempt to find cross section for the CCl x radicals. -- Abstract: We report here the total cross section for chloroflouromethanes namely CCl 3 F (Freon 11), CCl 2 F 2 (Freon 12), CClF 3 (Freon 13), CCl 4 and CCl x (x = 1–3), radicals by electron impact 50 to 5000 eV. The total inelastic and elastic cross sections are obtained employing a complex optical potential formalism and solving the Schrödinger equation through partial wave analysis. The total cross section is obtained as the sum of total inelastic and elastic cross sections. The results obtained are compared with the existing experimental and theoretical data, wherever available. The present result shows reasonable agreement with previous data. For the CCl x radicals, we have predicted the total cross sections for the first time. The data reported here has immense interest to atmospheric and technological plasma modelling

  18. Tissue Banking, Bioinformatics, and Electronic Medical Records: The Front-End Requirements for Personalized Medicine

    Science.gov (United States)

    Suh, K. Stephen; Sarojini, Sreeja; Youssif, Maher; Nalley, Kip; Milinovikj, Natasha; Elloumi, Fathi; Russell, Steven; Pecora, Andrew; Schecter, Elyssa; Goy, Andre

    2013-01-01

    Personalized medicine promises patient-tailored treatments that enhance patient care and decrease overall treatment costs by focusing on genetics and “-omics” data obtained from patient biospecimens and records to guide therapy choices that generate good clinical outcomes. The approach relies on diagnostic and prognostic use of novel biomarkers discovered through combinations of tissue banking, bioinformatics, and electronic medical records (EMRs). The analytical power of bioinformatic platforms combined with patient clinical data from EMRs can reveal potential biomarkers and clinical phenotypes that allow researchers to develop experimental strategies using selected patient biospecimens stored in tissue banks. For cancer, high-quality biospecimens collected at diagnosis, first relapse, and various treatment stages provide crucial resources for study designs. To enlarge biospecimen collections, patient education regarding the value of specimen donation is vital. One approach for increasing consent is to offer publically available illustrations and game-like engagements demonstrating how wider sample availability facilitates development of novel therapies. The critical value of tissue bank samples, bioinformatics, and EMR in the early stages of the biomarker discovery process for personalized medicine is often overlooked. The data obtained also require cross-disciplinary collaborations to translate experimental results into clinical practice and diagnostic and prognostic use in personalized medicine. PMID:23818899

  19. Is chronic fatigue syndrome a connective tissue disorder? A cross-sectional study in adolescents

    NARCIS (Netherlands)

    van de Putte, E. M.; Uiterwaal, C. S. P. M.; Bots, M. L.; Kuis, W.; Kimpen, J. L. L.; Engelbert, R. H. H.

    2005-01-01

    To investigate whether constitutional laxity of the connective tissues is more frequently present in adolescents with chronic fatigue syndrome (CFS) than in healthy controls. Increased joint hypermobility in patients with CFS has been previously described, as has lower blood pressure in fatigued

  20. Combined in situ zymography, immunofluorescence, and staining of iron oxide particles in paraffin-embedded, zinc-fixed tissue sections.

    Science.gov (United States)

    Haeckel, Akvile; Schoenzart, Lena; Appler, Franziska; Schnorr, Joerg; Taupitz, Matthias; Hamm, Bernd; Schellenberger, Eyk

    2012-01-01

    Superparamagnetic iron oxide particles are used as potent contrast agents in magnetic resonance imaging. In histology, these particles are frequently visualized by Prussian blue iron staining of aldehyde-fixed, paraffin-embedded tissues. Recently, zinc salt-based fixative was shown to preserve enzyme activity in paraffin-embedded tissues. In this study, we demonstrate that zinc fixation allows combining in situ zymography with fluorescence immunohistochemistry (IHC) and iron staining for advanced biologic investigation of iron oxide particle accumulation. Very small iron oxide particles, developed for magnetic resonance angiography, were applied intravenously to BALB/c nude mice. After 3 hours, spleens were explanted and subjected to zinc fixation and paraffin embedding. Cut tissue sections were further processed to in situ zymography, IHC, and Prussian blue staining procedures. The combination of in situ zymography as well as IHC with subsequent Prussian blue iron staining on zinc-fixed paraffin-embedded tissues resulted in excellent histologic images of enzyme activity, protease distribution, and iron oxide particle accumulation. The combination of all three stains on a single section allowed direct comparison with only moderate degradation of fluorescein isothiocyanate-labeled substrate. This protocol is useful for investigating the biologic environment of accumulating iron oxide particles, with excellent preservation of morphology.

  1. Quantum Cascade Laser-Based Infrared Microscopy for Label-Free and Automated Cancer Classification in Tissue Sections.

    Science.gov (United States)

    Kuepper, Claus; Kallenbach-Thieltges, Angela; Juette, Hendrik; Tannapfel, Andrea; Großerueschkamp, Frederik; Gerwert, Klaus

    2018-05-16

    A feasibility study using a quantum cascade laser-based infrared microscope for the rapid and label-free classification of colorectal cancer tissues is presented. Infrared imaging is a reliable, robust, automated, and operator-independent tissue classification method that has been used for differential classification of tissue thin sections identifying tumorous regions. However, long acquisition time by the so far used FT-IR-based microscopes hampered the clinical translation of this technique. Here, the used quantum cascade laser-based microscope provides now infrared images for precise tissue classification within few minutes. We analyzed 110 patients with UICC-Stage II and III colorectal cancer, showing 96% sensitivity and 100% specificity of this label-free method as compared to histopathology, the gold standard in routine clinical diagnostics. The main hurdle for the clinical translation of IR-Imaging is overcome now by the short acquisition time for high quality diagnostic images, which is in the same time range as frozen sections by pathologists.

  2. Electron beam welding of heavy section 3Cr-1.5Mo alloy

    International Nuclear Information System (INIS)

    King, J.F.; David, S.A.; Nasreldin, A.

    1986-01-01

    Welding of thick section steels is a common practice in the fabrication of pressure vessels for energy systems. The fabrication cost is strongly influenced by the speed at which these large components can be welded. Conventional welding processes such as shielded metal arc (SMA) and submerged arc (SA) are time-consuming and expensive. Hence there is a great need to reduce welding time and the tonnage of weld metal deposited. Electron beam welding (EBW) is a process that potentially could be used to achieve dramatic reduction in the welding time and costs. The penetrating ability of the beam produces welds with high depth-to-width ratios at relatively high travel speeds, making it possible to weld thick sections with one or two passes without filler metals and other consumables. The paper describes a study that was undertaken to investigate the feasibility of using a high power electron beam welding machine to weld heavy section steel. The main emphasis of this work was concentrated on determining the mechanical properties of the resulting weldment, characterizing the microstructure of the various weldment regions, and comparing these results with those from other processes. One of the steels selected for the heavy section electron beam welding study was a new 3 Cr-1.5 Mo-0.1 V alloy. The steel was developed at the AMAX Materials Research Center by Wada and co-workers for high temperature, high pressure hydrogen service as a possible improved replacement for 2-1/4 Cr-1 Mo steels. The excellent strength and toughness of this steel make it a promising candidate for future pressure vessels such as those for coal gasifiers. The work was conducted on 102 mm (4 in.) thick plates of this material in the normalized-and-tempered condition

  3. Charge-state dependence of binary-encounter-electron cross sections and peak energies

    International Nuclear Information System (INIS)

    Hidmi, H.I.; Richard, P.; Sanders, J.M.; Schoene, H.; Giese, J.P.; Lee, D.H.; Zouros, T.J.M.; Varghese, S.L.

    1993-01-01

    The charge-state dependence of the binary-encounter-electron (BEE) double-differential cross section (DDCS) at 0 degree with respect to the beam direction resulting from collisions of 1 MeV/amu H + , C q+ , N q+ , O q+ , F q+ , Si q+ , and Cl q+ , and 0.5 MeV/amu Cu q+ with H 2 is reported. The data show an enhancement in the BEE DDCS as the charge state of the projectile is decreased, in agreement with the data reported by Richard et al. [J. Phys. B 23, L213 (1990)]. The DDCS enhancement ratios observed for the three-electron isoelectronic sequence C 3+ :C 6+ , N 4+ :N 7+ , O 5+ :O 8+ , and F 6+ :F 9+ are about 1.35, whereas a DDCS enhancement of 3.5 was observed for Cu 4+ . The BEE enhancement with increasing electrons on the projectile has been shown by several authors to be due to the non-Coulomb static potential of the projectile and additionally to the e-e exchange interaction. An impulse-approximation (IA) model fits the shape of the BEE DDCS and predicts a Z p 2 dependence for the bare-ion cross sections. The IA also predicts a binary peak energy that is independent of q and Z p and below the classical value of 4t, where t is the energy of electrons traveling with the projectile velocity. We observed a BEE energy shift ΔE (ΔE=4t-E peak , where E peak is the measured energy at the peak of the binary encounter electrons) that is approximately independent of q for the low-Z p ions, whereas the measured ΔE values for Si, Cl, and Cu were found to be q dependent

  4. Absolute total and one and two electron transfer cross sections for Ar8+ on Ar as a function of energy

    International Nuclear Information System (INIS)

    Vancura, J.; Kostroun, V.O.

    1992-01-01

    The absolute total and one and two electron transfer cross sections for Ar 8+ on Ar were measured as a function of projectile laboratory energy from 0.090 to 0.550 keV/amu. The effective one electron transfer cross section dominates above 0.32 keV/amu, while below this energy, the effective two electron transfer starts to become appreciable. The total cross section varies by a factor over the energy range explored. The overall error in the cross section measurement is estimated to be ± 15%

  5. 17 CFR 230.160 - Registered investment company exemption from Section 101(c)(1) of the Electronic Signatures in...

    Science.gov (United States)

    2010-04-01

    ... exemption from Section 101(c)(1) of the Electronic Signatures in Global and National Commerce Act. 230.160...(c)(1) of the Electronic Signatures in Global and National Commerce Act. A prospectus for an... 101(c)(1) of the Electronic Signatures in Global and National Commerce Act. [65 FR 47284, Aug. 2, 2000] ...

  6. Bibliography of electron and photon cross sections with atoms and molecules. Published in the 20th century. Methane

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2004-10-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for methane (CH 4 ). About 1050 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1924 through 2000 for CH 4 . Finally, author's comments for CH 4 electron collision cross sections are given. (author)

  7. Double-continuum wave functions and double-photoionization cross sections of two-electron systems

    International Nuclear Information System (INIS)

    Tiwary, S.N.

    1996-09-01

    The present review briefly presents the growing experimental as well as theoretical interests in recent years in the double-continuum wave functions and double-photoionization cross sections of two-electron systems. The validity of existing double-continuum wave functions is analyzed and the importance of electronic correlations in both the initial as well as final states wave functions involved in the transition amplitude for double-photoionization process is demonstrated. At present, we do not have comprehensive and practical double-continuum wave functions which account the full correlation of two-electron in the continuum. Basic difficulties in making accurate theoretical calculations of double ionization by a single high energy photon especially in the vicinity of the threshold, where the correlation plays an important role, are discussed. Illuminating, illustrative and representative examples are presented in order to show the present status and the progress in this field. Future challenges and directions, in high-precision double-photoionization cross sections calculations, have been discussed and suggested. (author). 133 refs, 9 figs

  8. Quantum-mechanical predictions of electron-induced ionization cross sections of DNA components

    International Nuclear Information System (INIS)

    Champion, Christophe

    2013-01-01

    Ionization of biomolecules remains still today rarely investigated on both the experimental and the theoretical sides. In this context, the present work appears as one of the first quantum mechanical approaches providing a multi-differential description of the electron-induced ionization process of the main DNA components for impact energies ranging from the target ionization threshold up to about 10 keV. The cross section calculations are here performed within the 1st Born approximation framework in which the ejected electron is described by a Coulomb wave whereas the incident and the scattered electrons are both described by a plane wave. The biological targets of interest, namely, the DNA nucleobases and the sugar-phosphate backbone, are here described by means of the GAUSSIAN 09 system using the restricted Hartree-Fock method with geometry optimization. The theoretical predictions also obtained have shown a reasonable agreement with the experimental total ionization cross sections while huge discrepancies have been pointed out with existing theoretical models, mainly developed within a semi-classical framework.

  9. Electron impact excitation cross sections and rates from the ground state of atomic calcium

    CERN Document Server

    Samson, A M

    2001-01-01

    New R-matrix calculations are presented for electron excitation of atomic calcium. The target state expansion includes 22 states: 4s sup 2 sup 1 S; 4snl sup 1 sup , sup 3 L, where nl is 3d, 4p, 5s, 5p, 4d and 4f; 3d4p sup 1 sup , sup 3 P,D,F; and 4p sup 2 sup 3 P, sup 1 D, sup 1 S terms. The calculation is in LS coupling, and configuration interaction involving 3p subshell correlation is included. Electron impact excitation cross sections from the 4s sup 2 ground state to the next 10 states are tabulated for low energies, and thermally averaged effective collision strengths are tabulated over a range of electron temperatures from 1000 to 10,000 K. Comparisons are made with previous cross sections calculations for the 4s sup 2 -4s4p sup 3 P deg. transition; excellent agreement is found with experimentally derived rates for 4s sup 2 -4s4p sup 1 P deg

  10. Measurement of the hadronic cross section in electron-positron annihilation

    International Nuclear Information System (INIS)

    Clearwater, S.

    1983-11-01

    This thesis describes the most precise measurement to date of the ratio R, the hadronic cross section in lowest order electron-positron annihilation to the cross section for muon pair production in lowest order electron-positron annihilation. This experiment is of interest because R is a fundamental parameter that tests in a model independent way the basic assumptions of strong interaction theories. According to the assumptions of one of these theories the value of R is determined simply from the electric charges, spin, and color assignments of the produced quark-pairs. The experiment was carried out with the MAgnetic Calorimeter using collisions of 14.5 GeV electrons and positrons at the 2200m circumference PEP storage ring at SLAC. The MAC detector is one of the best-suited collider detectors for measuring R due to its nearly complete coverage of the full angular range. The data for this experiment were accumulated between February 1982 and April 1983 corresponding to a total event sample of about 40,000 hadronic events. About 5% of the data were taken with 14 GeV beams and the rest of the data were taken with 14.5 GeV beams. A description of particle interactions and experimental considerations is given

  11. Double- and triple-differential cross sections for electron-impact ionization of helium

    International Nuclear Information System (INIS)

    Biswas, R.; Sinha, C.

    1995-01-01

    Triple- (TDCS) and double- (DDCS) differential cross sections have been calculated for single ionization in electron-helium collisions for asymmetric geometry at intermediate and medium high energies. The TDCS and DDCS results have been presented for different kinematical situations and have been compared with the corresponding experiments. In the present prescription, the final-state wave function involves the correlation between the two continuum electrons and satisfies the three-body asymptotic boundary condition (for asymmetric geometry), which is an important criterion for reliable ionization cross sections. The sensitivity of the ionization cross sections (particularly of the TDCS) with respect to the choice of the bound-state wave function of the He atom has also been studied, using two different forms of wave function of the He atom. The binary-to-recoil peak intensity ratio against momentum transfer in TDCS is found to be in closer agreement with the experiment for the simple Hylleraas wave function than for the Hartree-Fock wave function. The DDCS results are found to be in good agreement with the experimental data of Mueller-Fiedler et al. [J. Phys. B 19, 1211 (1986)] for lower ejected energy (E 2 ), while for higher E 2 the results are closer to the measurements of Shyn et al. [Phys. Rev. A 19, 557 (1979)] and Avaldi et al. [Nuovo Cimento D 9, 97 (1987)

  12. Inelastic cross-sections for electron transport in liquid water: a comparison of dielectric models

    International Nuclear Information System (INIS)

    Emfietzoglou, D.

    2003-01-01

    Various methodologies for constructing inelastic cross-sections for low-energy (<10 keV) electron transport in liquid water are presented and compared. They are all based on an optical-data model which provides the dependence on energy loss, and a dispersion algorithm which incorporates the momentum-transfer dependence. A Drude dielectric model was used to analytically represent the optical data. Various dispersion schemes were examined: the Bethe approximation, the δ-oscillator models of Ashley and Liljequist, and two forms of Ritchie's extended-Drude model. They all have been used in Monte-Carlo (MC) codes for analog electron transport in the condensed phase. Results in the form of differential and total inelastic cross-sections are presented. Where possible, comparisons with results of other studies are made. It was found that, despite the application of general constraints (e.g. sum rules), the optical model has a notable influence on the single-collision energy loss spectrum. In addition, both the shape and peak position of the total and differential cross-section distributions depend strongly on the dispersion model adopted. The work is particularly relevant to the development of event-by-event MC transport codes for liquid water, as well as, to the calculations of stopping-powers below the range of applicability of Bethe's formula

  13. The effective differential cross section for elastic scattering of electrons by atoms and its use for Monte Carlo simulation of electron passage through matter

    International Nuclear Information System (INIS)

    Sheikin, E G

    2010-01-01

    The effective differential cross section (DCS) for elastic scattering of electrons by atoms is proposed that reproduces known energy dependences for the first and second transport cross sections but provides a total elastic cross section that is significantly small compared with the known energy dependences. The number of elastic collisions of electrons in matter when using the effective DCS in Monte Carlo simulations is significantly lower than that when using the real DCS. The results of our Monte Carlo simulation of electron propagation in aluminium using the proposed DCS are in good agreement with experimental data.

  14. Photodouble ionization differential cross sections for D2 with various electron energy sharing conditions

    International Nuclear Information System (INIS)

    Seccombe, D.P.; Collins, S.A.; Reddish, T.J.; Selles, P.; Malegat, L.; Huetz, A.; Kazansky, A.K.

    2002-01-01

    The mutual angular distributions of the two ejected electrons following direct photodouble ionization have been measured for D 2 at an excess energy (E) of 25 eV using linearly polarized light. These (γ, 2e) 'triple' differential cross sections (TDCSs) were obtained for asymmetric electron energy conditions with energy sharing ratios (R=E 2 /E 1 ) of R=24, 11.5, 4 and 2.57. In all cases the 'reference' electron (energy = E 1 ) was oriented along the direction of the electric field vector (ε) and detected in coincidence with a second electron (energy = E 2 ) coplanar with ε and the photon beam direction (kγ). For comparison, helium TDCSs were obtained for the same E and R values under nearly identical spectrometer conditions. These show very good agreement with the results of hyperspherical-R-matrix with semi-classical outgoing waves calculations, thus providing even more confidence in the D 2 TDCSs where there is as yet no accurate ab initio theory. The similarities and differences between the experimental results associated with the two targets are qualitatively discussed in terms of Feagin's model (Feagin J M 1998 J. Phys. B: At. Mol. Opt. Phys. 31 L729). (author)

  15. Cross-section transmission electron microscopy of the ion implantation damage in annealed diamond

    Energy Technology Data Exchange (ETDEWEB)

    Derry, T.E. [DST/NRF Centre of Excellence in Strong Materials and School of Physics, University of the Witwatersrand, Wits 2050, Johannesburg (South Africa)], E-mail: Trevor.Derry@wits.ac.za; Nshingabigwi, E.K. [DST/NRF Centre of Excellence in Strong Materials and School of Physics, University of the Witwatersrand, Wits 2050, Johannesburg (South Africa); Department of Physics, National University of Rwanda, P.O. Box 117, Huye (Rwanda); Levitt, M. [DST/NRF Centre of Excellence in Strong Materials and School of Physics, University of the Witwatersrand, Wits 2050, Johannesburg (South Africa); Neethling, J. [DST/NRF CoE-SM and Physics Department, Nelson Mandela Metropolitan University, Port Elizabeth (South Africa); Naidoo, S.R. [DST/NRF Centre of Excellence in Strong Materials and School of Physics, University of the Witwatersrand, Wits 2050, Johannesburg (South Africa)

    2009-08-15

    It has formerly been shown that low-damage levels, produced during the implantation doping of diamond as a semiconductor, anneal easily while high levels 'graphitize' (above about 5.2 x 10{sup 15} ions/cm{sup 2}). The difference in the defect types and their profiles, in the two cases, has never been directly observed. We have succeeded in using cross-section transmission electron microscopy to do so. The experiments were difficult because the specimens must be polished to {approx}40 {mu}m thickness, then implanted on edge and annealed, before final ion beam thinning to electron transparency. The low-damage micrographs reveal some deeply penetrating dislocations, whose existence had been predicted in earlier work.

  16. Electron capture to the continuum manifestation in fully differential cross sections for ion impact single ionization

    Science.gov (United States)

    Ciappina, M. F.; Fojón, O. A.; Rivarola, R. D.

    2018-04-01

    We present theoretical calculations of single ionization of He atoms by protons and multiply charged ions. The kinematical conditions are deliberately chosen in such a way that the ejected electron velocity matches the projectile impact velocity. The computed fully differential cross sections (FDCS) in the scattering plane using the continuum-distorted wave-eikonal initial state show a distinct peaked structure for a polar electron emission angle θ k = 0°. This element is absent when a first order theory is employed. Consequently, we can argue that this peak is a clear manifestation of a three-body effect, not observed before in FDCS. We discuss a possible interpretation of this new feature.

  17. Dissociative Excitation of Acetylene Induced by Electron Impact: Excitation-emission Cross-sections

    Energy Technology Data Exchange (ETDEWEB)

    Országh, Juraj; Danko, Marián; Čechvala, Peter; Matejčík, Štefan, E-mail: matejcik@fmph.uniba.sk [Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská dolina F-2, 842 48 Bratislava (Slovakia)

    2017-05-20

    The optical emission spectrum of acetylene excited by monoenergetic electrons was studied in the range of 190–660 nm. The dissociative excitation and dissociative ionization associated with excitation of the ions initiated by electron impact were dominant processes contributing to the spectrum. The spectrum was dominated by the atomic lines (hydrogen Balmer series, carbon) and molecular bands (CH(A–X), CH(B–X), CH{sup +}(B–A), and C{sub 2}). Besides the discrete transitions, we have detected the continuum emission radiation of ethynyl radical C{sub 2}H(A–X). For most important lines and bands of the spectrum we have measured absolute excitation-emission cross sections and determined the energy thresholds of the particular dissociative channels.

  18. Optical depth sectioning in the aberration-corrected scanning transmission and scanning confocal electron microscope

    International Nuclear Information System (INIS)

    Behan, G; Nellist, P D

    2008-01-01

    The use of spherical aberration correctors in the scanning transmission electron microscope (STEM) has the effect of reducing the depth of field of the microscope, making three-dimensional imaging of a specimen possible by optical sectioning. Depth resolution can be improved further by placing aberration correctors and lenses pre and post specimen to achieve an imaging mode known as scanning confocal electron microscopy (SCEM). We present the calculated incoherent point spread functions (PSF) and optical transfer functions (OTF) of a STEM and SCEM. The OTF for a STEM is shown to have a missing cone region which results in severe blurring along the optic axis, which can be especially severe for extended objects. We also present strategies for reconstruction of experimental data, such as three-dimensional deconvolution of the point spread function.

  19. Cross-section transmission electron microscopy of the ion implantation damage in annealed diamond

    International Nuclear Information System (INIS)

    Derry, T.E.; Nshingabigwi, E.K.; Levitt, M.; Neethling, J.; Naidoo, S.R.

    2009-01-01

    It has formerly been shown that low-damage levels, produced during the implantation doping of diamond as a semiconductor, anneal easily while high levels 'graphitize' (above about 5.2 x 10 15 ions/cm 2 ). The difference in the defect types and their profiles, in the two cases, has never been directly observed. We have succeeded in using cross-section transmission electron microscopy to do so. The experiments were difficult because the specimens must be polished to ∼40 μm thickness, then implanted on edge and annealed, before final ion beam thinning to electron transparency. The low-damage micrographs reveal some deeply penetrating dislocations, whose existence had been predicted in earlier work.

  20. Absolute cross sections for the ionization-excitation of helium by electron impact

    Science.gov (United States)

    Bellm, S.; Lower, J.; Weigold, E.; Bray, I.; Fursa, D. V.; Bartschat, K.; Harris, A. L.; Madison, D. H.

    2008-09-01

    In a recent publication we presented detailed experimental and theoretical results for the electron-impact-induced ionization of ground-state helium atoms. The purpose of that work was to refine theoretical approaches and provide further insight into the Coulomb four-body problem. Cross section ratios were presented for transitions leading to excited states, relative to those leading to the ground state, of the helium ion. We now build on that study by presenting individual relative triple-differential ionization cross sections (TDCSs) for an additional body of experimental data measured at lower values of scattered-electron energies. This has been facilitated through the development of new electron-gun optics which enables us to accurately characterize the spectrometer transmission at low energies. The experimental results are compared to calculations resulting from a number of different approaches. For ionization leading to He+(1s2)1S , cross sections are calculated by the highly accurate convergent close-coupling (CCC) method. The CCC data are used to place the relative experimental data on to an absolute scale. TDCSs describing transitions to the excited states are calculated through three different approaches, namely, through a hybrid distorted- wave+R -matrix (close-coupling) model, through the recently developed four-body distorted-wave model, and by a first Born approximation calculation. Comparison of the first- and second-order theories with experiment allows for the accuracy of the different theoretical approaches to be assessed and gives insight into which physical aspects of the problem are most important to accurately model.

  1. Single electron capture differential cross section in H+ + He collisions at intermediate and high collision energies

    International Nuclear Information System (INIS)

    Abufager, P N; Fainstein, P D; MartInez, A E; Rivarola, R D

    2005-01-01

    The generalized continuum distorted wave-eikonal initial state (CDW-EIS II) approximation is employed to study differential cross sections (DCS) for single electron capture in H + + He collisions at intermediate and high energies. Present results are compared with theoretical calculations obtained using the previous CDW-EIS formulation in order to show the importance of the description of the bound and continuum target states in the entrance and exit channels, respectively. Both DCS are also shown together with other theoretical results and with experimental data

  2. Electron scattering on N2O-from cross sections to diffusion coefficients

    International Nuclear Information System (INIS)

    Mechlinska-Drewko, J.; Wroblewski, T.; Petrovic, Z.L.; Novakovic, V.; Karwasz, G.P.

    2003-01-01

    Results of measurements of the ratio of transverse (D T /μ) and longitudinal (D L /μ) diffusion coefficients to mobility and drift velocity (W) as function of reduced electrical field (E/N) for electrons in nitrous oxide are presented. The coefficients D T /μ and D L /μ have been determined by applying the Townsend-Huxley method. The drift velocities were obtained by using the Bradbury-Nielsen technique. Also the deduced set of total and partial cross sections has been used to calculate the D T /μ and W

  3. Electron-Impact Excitation Cross Sections for Modeling Non-Equilibrium Gas

    Science.gov (United States)

    Huo, Winifred M.; Liu, Yen; Panesi, Marco; Munafo, Alessandro; Wray, Alan; Carbon, Duane F.

    2015-01-01

    In order to provide a database for modeling hypersonic entry in a partially ionized gas under non-equilibrium, the electron-impact excitation cross sections of atoms have been calculated using perturbation theory. The energy levels covered in the calculation are retrieved from the level list in the HyperRad code. The downstream flow-field is determined by solving a set of continuity equations for each component. The individual structure of each energy level is included. These equations are then complemented by the Euler system of equations. Finally, the radiation field is modeled by solving the radiative transfer equation.

  4. Kuang's Semi-Classical Formalism for Calculating Electron Capture Cross Sections: A Space- Physics Application

    Science.gov (United States)

    Barghouty, A. F.

    2014-01-01

    Accurate estimates of electroncapture cross sections at energies relevant to the modeling of the transport, acceleration, and interaction of energetic neutral atoms (ENA) in space (approximately few MeV per nucleon) and especially for multi-electron ions must rely on detailed, but computationally expensive, quantum-mechanical description of the collision process. Kuang's semi-classical approach is an elegant and efficient way to arrive at these estimates. Motivated by ENA modeling efforts for apace applications, we shall briefly present this approach along with sample applications and report on current progress.

  5. Computation of electron-impact K-shell ionization cross sections of atoms

    International Nuclear Information System (INIS)

    Uddin, M.A.; Haque, A.K.F.; Billah, M. Masum; Basak, A.K.; Karim, K.R.; Saha, B.C.

    2005-01-01

    The total cross sections of electron impact single K-shell ionization of atomic targets, with a wide range of atomic numbers from Z=6-50, are evaluated in the energy range up to about 10 MeV employing the recently proposed modified version of the improved binary-encounter dipole (RQIBED) model [Uddin et al., Phys. Rev. A 70, 032706 (2004)], which incorporates the ionic and relativistic effects. The experimental cross sections for all targets are reproduced satisfactorily even in the relativistic energies using fixed generic values of the two parameters in the RQIBED model. The relativistic effect is found to be significant in all targets except for C, being profound in Ag and Sn

  6. Investigation of the influence of intermolecular interactions on the electronic stopping cross sections

    International Nuclear Information System (INIS)

    Krotz, R.; Neuwirth, W.; Pietsch, W.

    1980-01-01

    The electronic stopping cross sections for Li projectiles have been measured in various kinds of targets. They are analyzed here with respect to the different types of interactions between the constituents of the target: interactions between the atoms in a compound (chemical bonding), the ion-dipole interaction, if the target is an electrolytic solution, and the dipole-dipole interaction among polar molecules. The influence on the stopping cross section depends on the strength of these interactions; it varies from a few percent in the latter case up to 20% and more in a compound. These influences are the largest, if the velocity of the projectile is of the order of the average orbital velocity of the target atoms. (author)

  7. Differential and total cross sections for the ionization of water molecule by electron impact

    International Nuclear Information System (INIS)

    Houamer, S.; Dal Cappello, C.; Mansouri, A.

    2007-01-01

    A theoretical approach is presented to calculate multiply differential and total cross sections of the ionization of H 2 O molecule in the vapour phase. The wave function of the target is described by molecular orbitals consisting of a linear combination of slater type atomic orbitals centered on the heaviest atom which is the oxygen atom in this case. The calculations are carried out in the first Born approximation where the projectile is described by a plane wave while the ejected electron is described by a coulomb wave taking into account its interaction with the residual ion. The spherical average over the Euler solid angle due to the randomly oriented gaseous target molecule is carried out analytically using the rotation matrix properties. The differential and total cross sections are thus evaluated without any special difficulty and compared with experiments and distorted wave calculations. Fair agreements are observed

  8. Electron ionization of open/closed chain isocarbonic molecules relevant in plasma processing: Theoretical cross sections

    International Nuclear Information System (INIS)

    Patel, Umang R.; Joshipura, K. N.; Pandya, Siddharth H.; Kothari, Harshit N.

    2014-01-01

    In this paper, we report theoretical electron impact ionization cross sections from threshold to 2000 eV for isocarbonic open chain molecules C 4 H 6 , C 4 H 8 , C 4 F 6 including their isomers, and closed chain molecules c-C 4 H 8 and c-C 4 F 8 . Theoretical formalism employed presently, viz., Complex Scattering Potential-ionization contribution method has been used successfully for a variety of polyatomic molecules. The present ionization calculations are very important since results available for the studied targets are either scarce or none. Our work affords comparison of C 4 containing hydrocarbon versus fluorocarbon molecules. Comparisons of the present ionization cross sections are made wherever possible, and new ionization data are also presented

  9. Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections.

    Science.gov (United States)

    Adzemovic, Milena Z; Zeitelhofer, Manuel; Leisser, Marianne; Köck, Ulricke; Kury, Angela; Olsson, Tomas

    2016-11-14

    Immunohistochemistry (IHC) provides highly specific, reliable and attractive protein visualization. Correct performance and interpretation of an IHC-based multicolor labeling is challenging, especially when utilized for assessing interrelations between target proteins in the tissue with a high fat content such as the central nervous system (CNS). Our protocol represents a refinement of the standard immunolabeling technique particularly adjusted for detection of both structural and soluble proteins in the rat CNS and peripheral lymph nodes (LN) affected by neuroinflammation. Nonetheless, with or without further modifications, our protocol could likely be used for detection of other related protein targets, even in other organs and species than here presented.

  10. Determination of nitrosourea compounds in brain tissue by gas chromatography and electron capture detection.

    Science.gov (United States)

    Hassenbusch, S J; Colvin, O M; Anderson, J H

    1995-07-01

    A relatively simple, high-sensitivity gas chromatographic assay is described for nitrosourea compounds, such as BCNU [1,3-bis(2-chloroethyl)-1-nitrosourea] and MeCCNU [1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea], in small biopsy samples of brain and other tissues. After extraction with ethyl acetate, secondary amines in BCNU and MeCCNU are derivatized with trifluoroacetic anhydride. Compounds are separated and quantitated by gas chromatography using a capillary column with temperature programming and an electron capture detector. Standard curves of BCNU indicate a coefficient of variance of 0.066 +/- 0.018, a correlation coefficient of 0.929, and an extraction efficiency from whole brain of 68% with a minimum detectable amount of 20 ng in 5-10 mg samples. The assay has been facile and sensitive in over 1000 brain biopsy specimens after intravenous and intraarterial infusions of BCNU.

  11. Magnetic-sublevel cross sections for excitation of the n 1P levels of helium by electron impact

    International Nuclear Information System (INIS)

    Csanak, G.; Cartwright, D.C.; Trajmar, S.

    1992-01-01

    First-order many-body theory has been used to calculate collision-frame magnetic-sublevel differential cross sections for electron-impact excitation of the n 1 P (n=2,3,4,5,6) levels of helium for electrons with incident energy in the 25--500-eV range. By combining results from electron-impact differential-cross-section measurements and electron-photon coincidence measurements, experimental magnetic-sublevel cross sections have also been derived for the excitation of the 2 1 P and 3 1 P levels. The theory predicts a pronounced minimum for the M=0 magnetic-sublevel differential cross section for incident electron energies around 30 eV. Our theoretical results are compared to the experimental data and some other theoretical results

  12. Method to characterize inorganic particulates in lung tissue biopsies using field emission scanning electron microscopy

    Science.gov (United States)

    Lowers, Heather; Breit, George N.; Strand, Matthew; Pillers, Renee M.; Meeker, Gregory P.; Todorov, Todor I.; Plumlee, Geoffrey S.; Wolf, Ruth E.; Robinson, Maura; Parr, Jane; Miller, Robert J.; Groshong, Steve; Green, Francis; Rose, Cecile

    2018-01-01

    Humans accumulate large numbers of inorganic particles in their lungs over a lifetime. Whether this causes or contributes to debilitating disease over a normal lifespan depends on the type and concentration of the particles. We developed and tested a protocol for in situ characterization of the types and distribution of inorganic particles in biopsied lung tissue from three human groups using field emission scanning electron microscopy (FE-SEM) combined with energy dispersive spectroscopy (EDS). Many distinct particle types were recognized among the 13 000 particles analyzed. Silica, feldspars, clays, titanium dioxides, iron oxides and phosphates were the most common constituents in all samples. Particles were classified into three general groups: endogenous, which form naturally in the body; exogenic particles, natural earth materials; and anthropogenic particles, attributed to industrial sources. These in situ results were compared with those using conventional sodium hypochlorite tissue digestion and particle filtration. With the exception of clays and phosphates, the relative abundances of most common particle types were similar in both approaches. Nonetheless, the digestion/filtration method was determined to alter the texture and relative abundances of some particle types. SEM/EDS analysis of digestion filters could be automated in contrast to the more time intensive in situ analyses.

  13. Dosimetric evaluation in heterogeneous tissue of anterior electron beam irradiation for treatment of retinoblastoma

    International Nuclear Information System (INIS)

    Kirsner, S.M.; Hogstrom, K.R.; Kurup, R.G.; Moyers, M.F.

    1987-01-01

    A dosimetric study of anterior electron beam irradiation for treatment of retinoblastoma was performed to evaluate the influence of tissue heterogeneities on the dose distribution within the eye and the accuracy of the dose calculated by a pencil beam algorithm. Film measurements were made in a variety of polystyrene phantoms and in a removable polystyrene eye incorporated into a tissue substitute phantom constructed from a human skull. Measurements in polystyrene phantoms were used to demonstrate the algorithm's ability to predict the effect of a lens block placed in the beam, as well as the eye's irregular surface shape. The eye phantom was used to measure dose distributions within the eye in both the sagittal and transverse planes in order to test the algorithm's ability to predict the dose distribution when bony heterogeneities are present. Results show (1) that previous treatment planning conclusions based on flat, uniform phantoms for central-axis depth dose are adequate; (2) that a three-dimensional heterogeneity correction is required for accurate dose calculations; and (3) that if only a two-dimensional heterogeneity correction is used in calculating the dose, it is more accurate for the sagittal than the transverse plane

  14. s-wave threshold in electron attachment - Observations and cross sections in CCl4 and SF6 at ultralow electron energies

    Science.gov (United States)

    Chutjian, A.; Alajajian, S. H.

    1985-01-01

    The threshold photoionization method was used to study low-energy electron attachment phenomena in and cross sections of CCl4 and SF6 compounds, which have applications in the design of gaseous dielectrics and diffuse discharge opening switches. Measurements were made at electron energies from below threshold to 140 meV at resolutions of 6 and 8 meV. A narrow resolution-limited structure was observed in electron attachment to CCl4 and SF6 at electron energies below 10 meV, which is attributed to the divergence of the attachment cross section in the limit epsilon, l approaches zero. The results are compared with experimental collisional-ionization results, electron-swarm unfolded cross sections, and earlier threshold photoionization data.

  15. Cross sections and oscillator strengths for electron-impact excitation of electronic states in polyatomic molecules. Application examples of the BEf- scaling model in optically-allowed transitions

    International Nuclear Information System (INIS)

    Kato, H.; Kawahara, H.; Hoshino, M.

    2009-12-01

    Integral cross sections for optically allowed electronic-state excitations by electron impact, are reviewed for polyatomic molecules by applying the Binary-Encounter-Bethe (BEB) scaling model. Following the context of the present review, the scaling model originally proposed by Yong-Ki Kim to determine electron-impact cross sections for ionization of atoms and molecules is also summarized briefly for its wide range of applications [Electron-Impact Cross Section Database, NIST, Y.-K. Kim]. The present report not only focuses on the need for the cross-section data, but also elucidates the verification of the scaling model in the general application for atoms and molecules. Since this report is for a data base, it is summarized for data base users by citing (copying) the descriptions in the original papers and the references within those papers in the style of a textbook. (author)

  16. The analysis for energy distribution and biological effects of the clusters from electrons in the tissue equivalent material

    International Nuclear Information System (INIS)

    Zhang Wenzhong; Guo Yong; Luo Yisheng; Wang Yong

    2004-01-01

    Objective: To study energy distribution of the clusters from electrons in the tissue equivalent material, and discuss the important aspects of these clusters on inducing biological effects. Methods: Based on the physical mechanism for electrons interacting with tissue equivalent material, the Monte Carlo (MC) method was used. The electron tracks were lively simulated on an event-by-event (ionization, excitation, elastic scattering, Auger electron emission) basis in the material. The relevant conclusions were drawn from the statistic analysis of these events. Results: The electrons will deposit their energy in the form (30%) of cluster in passing through tissue equivalent material, and most clusters (80%) have the energy amount of more than 50 eV. The cluster density depends on its diameter and energy of electrons, and the deposited energy in the cluster depends on the type and energy of radiation. Conclusion: The deposited energy in cluster is the most important factor in inducing all sort of lesions on DNA molecules in tissue cells

  17. Electronic equilibrium as a function of depth in tissue from cobalt-60 point source exposures

    International Nuclear Information System (INIS)

    Myrick, J.A.

    1994-08-01

    The Nuclear Regulatory Commission has set the basic criteria for assessing skin dose stemming from hot particle contaminations. Compliance with 10 CFR 20.101 requires that exposure to the skin be evaluated over a 1 cm 2 area at a depth of 0.007 cm. Skin exposure can arise from both the beta and gamma components of radioactive particles and gamma radiation can contribute significantly to skin doses. The gamma component of dose increases dramatically when layers of protective clothing are interposed between the hot particle source and the skin, and in cases where the hot particle is large in comparison to the range of beta particles. Once the protective clothing layer is thicker than the maximum range of the beta particles, skin dose is due solely to gamma radiation. Charged particle equilibrium is not established at shallow depths. The degree of electronic equilibrium establishment must be assessed for shallow doses to prevent the over-assessment of skin dose because conventional fluence-to-dose conversion factors are not applicable. To assess the effect of electronic equilibrium, selected thicknesses of tissue equivalent material were interposed between radiochromic dye film and a 60 Co hot particle source and dose was measured as a function of depth. These measured values were then compared to models which are used to calculate charged particle equilibrium. The Miller-Reece model was found to agree closely with the experimental data while the Lantz-Lambert model overestimated dose at shallow depths

  18. Transmission electron microscopy of heart and liver tissues from rats fed with gums arabic and tragacanth.

    Science.gov (United States)

    Anderson, D M; Ashby, P; Busuttil, A; Kempson, S A; Lawson, M E

    1984-04-01

    Transmission electron microscopy has been used to examine the ultrastructure of rat hearts and livers after diet supplementation with (a) 0, 0.5, 1.5, 2.5 and 3.5% (w/w) gum tragacanth (GT) for 91 days, (b) 0 and 1% GT for 5 days (c) 0, 1, 4 and 8% (w/w) gum arabic (GA) for 28 days. The preparation and scrutiny of the electron micrographs was undertaken by two independent teams of specialists. There were no detectable abnormalities in any of the organelles in the heart and liver specimens from any of the test animals and no inclusions nor other pathological changes were observed. All micrographs showed normal, healthy tissues; particular attention was given to the mitochondria in hepatocytes as they serve as sensitive indicators of the health and state of activity of cells. In addition, the data obtained from assays of the microsomal protein and cytochrome P-450 content of the livers showed that GA and GT did not cause inductive effects. These results do not support earlier suggestions, based on in vitro assays, that GA and GT cause changes in the function of rat heart and liver mitochondria and liver microsomes; however, they confirm a report by Zbinden that the ingestion of GT does not produce abnormalities in the cardiac function of rats.

  19. Human brain receptor autoradiography using whole hemisphere sections: a general method that minimizes tissue artefacts

    International Nuclear Information System (INIS)

    Quirion, R.; Robitaille, Y.; Martial, J.; Chabot, J.G.; Lemoine, P.; Pilapil, C.; Dalpe, M.

    1987-01-01

    A general method for the preparation of high-quality, mostly ice-crystal-artefact-free whole human brain hemisphere sections is described. Upon receipt, hemispheres are divided; one is then fixed in buffered 10% formalin for neuropathological analysis while the other is cut in 8-10-mm-thick coronal slices that are then rapidly frozen in 2-methylbutane at -40 degrees C (10-15 sec) before being placed in the brain bank at -80 degrees C. Such rapid freezing markedly decreases the formation of ice-crystal artefacts. Whole-hemisphere 20-micron thick sections are then cut and mounted onto lantern-type gelatin-coated slides. These sections are subsequently used for both qualitative and quantitative in vitro receptor autoradiography. Examples of data obtained are given by using various radioligands labelling classical neutrotransmitter, neuropeptide, enzyme, and ion channel receptor binding sites. This method should be useful for the obtention of various receptor maps in human brain. Such information could be most useful for in vivo receptor visualization studies using positron emission tomography (PET) scanning. It could also indicate if a given receptor population is specifically and selectively altered in certain brain diseases, eventually leading to the development of new therapeutic approaches

  20. Measurements of absolute M-subshell X-ray production cross sections of Th by electron impact

    Energy Technology Data Exchange (ETDEWEB)

    Moy, A., E-mail: aurelien.moy@cea.fr [GM, CNRS, Université de Montpellier II, Place E. Bataillon, F-34095 Montpellier (France); CEA, DEN, DTEC, SGCS, LMAC, F-30207 Bagnols-sur-Cèze (France); Merlet, C. [GM, CNRS, Université de Montpellier II, Place E. Bataillon, F-34095 Montpellier (France); Dugne, O. [CEA, DEN, DTEC, SGCS, LMAC, F-30207 Bagnols-sur-Cèze (France)

    2014-08-31

    Highlights: • The M X-ray production cross sections of Th were measured by electron impact. • The M-subshell ionization cross sections of Th were determined from 3 to 38 keV. • Theoretical ionization cross-sections are in agreement with our experimental results. - Abstract: Measurements of absolute M-subshell X-ray production cross sections for element Th were made by electron impact for energies ranging from the ionization threshold up to 38 keV. Experimental data were obtained by measuring the X-ray intensity emitted from ultrathin Th films deposited onto self-supporting C backing films. The measurements were conducted with an electron microprobe using high-resolution wavelength dispersive spectrometers. Recorded intensities were converted into absolute X-ray production cross sections by means of atomic data and estimation of the number of primary electrons, target thickness, and detector efficiency. Our experimental X-ray production cross sections, the first to be reported for the M subshells of Th, are compared with X-ray production cross sections calculated with the mean of ionization cross sections obtained from the distorted-wave Born approximation. The Mα X-ray production cross section calculated is in excellent agreement with the measurements, allowing future use for standardless quantification in electron probe microanalysis.

  1. Bioimaging of metallothioneins in ocular tissue sections by laser ablation-ICP-MS using bioconjugated gold nanoclusters as specific tags.

    Science.gov (United States)

    Cruz-Alonso, María; Fernandez, Beatriz; Álvarez, Lydia; González-Iglesias, Héctor; Traub, Heike; Jakubowski, Norbert; Pereiro, Rosario

    2017-12-18

    An immunohistochemical method is described to visualize the distribution of metallothioneins 1/2 (MT 1/2) and metallothionein 3 (MT 3) in human ocular tissue. It is making use of (a) antibodies conjugated to gold nanoclusters (AuNCs) acting as labels, and (b) laser ablation (LA) coupled to inductively coupled plasma - mass spectrometry (ICP-MS). Water-soluble fluorescent AuNCs (with an average size of 2.7 nm) were synthesized and then conjugated to antibody by carbodiimide coupling. The surface of the modified AuNCs was then blocked with hydroxylamine to avoid nonspecific interactions with biological tissue. Immunoassays for MT 1/2 and MT 3 in ocular tissue sections (5 μm thick) from two post mortem human donors were performed. Imaging studies were then performed by fluorescence using confocal microscopy, and LA-ICP-MS was performed in the retina to measure the signal for gold. Signal amplification by the >500 gold atoms in each nanocluster allowed the antigens (MT 1/2 and MT 3) to be imaged by LA-ICP-MS using a laser spot size as small as 4 μm. The image patterns found in retina are in good agreement with those obtained by conventional fluorescence immunohistochemistry which was used as an established reference method. Graphical abstract Gold nanoclusters (AuNCs) conjugated to a primary specific antibody serve as a label for amplified bioimaging of metallothioneins (MTs) by laser ablation coupled to inductively coupled plasma - mass spectrometry (ICP-MS) in human ocular tissue sections.

  2. Electron impact ionization of B-like ion N2+. Resonance enhancement of the single-channel cross section

    International Nuclear Information System (INIS)

    Li Guohe; Qian Xingzhong; Pan Soufu

    1998-01-01

    The electron impact ionization cross sections of B-like ion N 2+ are calculated in the Coulomb-Born no exchange approximation by using R-matrix method, and the single differential cross section is given. The calculated results exhibit the Rydberg series of resonances. The resonance enhancement of the single-channel cross section is significantly greater than direct ionization cross section. It is agreement with that of Chidichimo

  3. Cross Sections for Electron-Impact Dissociation of Alternative Etching Gas, C3HF7O

    Science.gov (United States)

    Tanaka, Hideyuki; Toyoda, Hirotaka; Sugai, Hideo

    1998-09-01

    The search for alternative for perfluorocarbon gases from the environmental point of view has resulted in a new etching gas, C3HF7O (1,2,2,2-tetrafluoroethyl-trifluoromethyl ether, abbreviated as HFE-227). In this paper, the first measurement of the absolute cross sections for the dissociation of HFE-227, is reported.The neutral dissociation is measured from the threshold to 250 eV by appearance mass spectrometry in a dual electron beam device.The threshold energies for the neutral dissociation into CF, CF2 and CF3 are 14.7, 12.5 and 11.2 eV, respectively.The cross sections for the dissociation from HFE-227 into CF3 and CF are larger than those from c-C4F8 (octafluorocyclobutane), but not for the dissociation into CF2.Besides the neutral dissociation, the cross sections for the dissociative ionization of HFE-227 are extensively measured for the formation of twelve ionic species, i.e., CO+, CHO+, CF+, CHF+, CFO+, CF2+, CHF2+, CF3+, CHF3+, C2HF2O+, C2HF4+ and C2HF4O+.

  4. Physical models, cross sections, and numerical approximations used in MCNP and GEANT4 Monte Carlo codes for photon and electron absorbed fraction calculation.

    Science.gov (United States)

    Yoriyaz, Hélio; Moralles, Maurício; Siqueira, Paulo de Tarso Dalledone; Guimarães, Carla da Costa; Cintra, Felipe Belonsi; dos Santos, Adimir

    2009-11-01

    Radiopharmaceutical applications in nuclear medicine require a detailed dosimetry estimate of the radiation energy delivered to the human tissues. Over the past years, several publications addressed the problem of internal dose estimate in volumes of several sizes considering photon and electron sources. Most of them used Monte Carlo radiation transport codes. Despite the widespread use of these codes due to the variety of resources and potentials they offered to carry out dose calculations, several aspects like physical models, cross sections, and numerical approximations used in the simulations still remain an object of study. Accurate dose estimate depends on the correct selection of a set of simulation options that should be carefully chosen. This article presents an analysis of several simulation options provided by two of the most used codes worldwide: MCNP and GEANT4. For this purpose, comparisons of absorbed fraction estimates obtained with different physical models, cross sections, and numerical approximations are presented for spheres of several sizes and composed as five different biological tissues. Considerable discrepancies have been found in some cases not only between the different codes but also between different cross sections and algorithms in the same code. Maximum differences found between the two codes are 5.0% and 10%, respectively, for photons and electrons. Even for simple problems as spheres and uniform radiation sources, the set of parameters chosen by any Monte Carlo code significantly affects the final results of a simulation, demonstrating the importance of the correct choice of parameters in the simulation.

  5. Analysis of Data on the Cross Sections for Electron-Impact Ionization and Excitation of Electronic States of Atomic Hydrogen (Review)

    Science.gov (United States)

    Shakhatov, V. A.; Lebedev, Yu. A.

    2018-01-01

    A review is given of experimental and theoretical data on the cross sections for ionization, excitation, and deexcitation of atomic hydrogen. The set of the cross sections required to calculate the electron energy distribution function and find the level-to-level rate coefficients needed to solve balance equations for the densities of neutral and charged particles in hydrogen plasma is determined.

  6. SOFT TISSUE SARCOMAS – A CROS S SECTIONAL STUDY AT MNJIO

    Directory of Open Access Journals (Sweden)

    Ramesh

    2015-07-01

    Full Text Available BACKGROUND: Sarcomas are an uncommon group of cancers with a wide distribution in the various anatomical regions of the body and even wider and confusing range of histopathological diagnosis. AIMS: To study the mode of presentation, histology, treatment modalities use d and outcomes both in terms of function and disease free survivals of all patients treated in the Department of surgical oncology in our Institute. MATERIALS & METHODS: All patients of soft tissue sarcoma presenting to out department were studied and data collected in a proforma. Histology was established by Trucut/open biopsy. Patients after clinical examination were evaluated f or distant disease in lungs by x - ray chest followed by CT scan . RESULTS: Symptomatology and anatomical location in our study was similar to world literature but stage of presentation and tumour size is advanced in our study. CONCLUSIONS: Patients in our study presented late, probably due to lack of access to medical care, delay in diagnosis and delay in referral. Hence higher incidence of amputations and also higher incidence of need for reconstruction. Successful utilization of multi - modality treatment and availability of good reconstruction ensured good functional outcome.

  7. Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography.

    Science.gov (United States)

    Haraguchi, Yuji; Hasegawa, Akiyuki; Matsuura, Katsuhisa; Kobayashi, Mari; Iwana, Shin-Ichi; Kabetani, Yasuhiro; Shimizu, Tatsuya

    2017-01-01

    Three-dimensional (3D) tissues are engineered by stacking cell sheets, and these tissues have been applied in clinical regenerative therapies. The optimal fabrication technique of 3D human tissues and the real-time observation system for these tissues are important in tissue engineering, regenerative medicine, cardiac physiology, and the safety testing of candidate chemicals. In this study, for aiming the clinical application, 3D human cardiac tissues were rapidly fabricated by human induced pluripotent stem (iPS) cell-derived cardiac cell sheets with centrifugation, and the structures and beatings in the cardiac tissues were observed cross-sectionally and noninvasively by two optical coherence tomography (OCT) systems. The fabrication time was reduced to approximately one-quarter by centrifugation. The cross-sectional observation showed that multilayered cardiac cell sheets adhered tightly just after centrifugation. Additionally, the cross-sectional transmissions of beatings within multilayered human cardiac tissues were clearly detected by OCT. The observation showed the synchronous beatings of the thicker 3D human cardiac tissues, which were fabricated rapidly by cell sheet technology and centrifugation. The rapid tissue-fabrication technique and OCT technology will show a powerful potential in cardiac tissue engineering, regenerative medicine, and drug discovery research.

  8. Total and single differential cross sections for the electron impact ionization of the ground state of helium

    International Nuclear Information System (INIS)

    Singh, T.S.C.; Choudhury, K.B.; Singh, M.B.; Deb, N.C.; Mukherjee, S.C.; Mazumdar, P.S.

    1997-01-01

    Total cross sections (TCS) and single differential cross sections (SDCS) have been computed for the single ionization of the ground state of helium by electron impact in a distorted wave formalism which takes into account the effects of the initial and final channel distortions. The present TCS and SDCS results are in fair agreement with the measured values and other theoretical predictions for the incident electron energy E i > 150 eV. (orig.)

  9. Pointwise mutual information quantifies intratumor heterogeneity in tissue sections labeled with multiple fluorescent biomarkers

    Directory of Open Access Journals (Sweden)

    Daniel M Spagnolo

    2016-01-01

    Full Text Available Background: Measures of spatial intratumor heterogeneity are potentially important diagnostic biomarkers for cancer progression, proliferation, and response to therapy. Spatial relationships among cells including cancer and stromal cells in the tumor microenvironment (TME are key contributors to heterogeneity. Methods: We demonstrate how to quantify spatial heterogeneity from immunofluorescence pathology samples, using a set of 3 basic breast cancer biomarkers as a test case. We learn a set of dominant biomarker intensity patterns and map the spatial distribution of the biomarker patterns with a network. We then describe the pairwise association statistics for each pattern within the network using pointwise mutual information (PMI and visually represent heterogeneity with a two-dimensional map. Results: We found a salient set of 8 biomarker patterns to describe cellular phenotypes from a tissue microarray cohort containing 4 different breast cancer subtypes. After computing PMI for each pair of biomarker patterns in each patient and tumor replicate, we visualize the interactions that contribute to the resulting association statistics. Then, we demonstrate the potential for using PMI as a diagnostic biomarker, by comparing PMI maps and heterogeneity scores from patients across the 4 different cancer subtypes. Estrogen receptor positive invasive lobular carcinoma patient, AL13-6, exhibited the highest heterogeneity score among those tested, while estrogen receptor negative invasive ductal carcinoma patient, AL13-14, exhibited the lowest heterogeneity score. Conclusions: This paper presents an approach for describing intratumor heterogeneity, in a quantitative fashion (via PMI, which departs from the purely qualitative approaches currently used in the clinic. PMI is generalizable to highly multiplexed/hyperplexed immunofluorescence images, as well as spatial data from complementary in situ methods including FISSEQ and CyTOF, sampling many different

  10. Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections.

    Science.gov (United States)

    Krzyzanowska, Agnieszka; Lippolis, Giuseppe; Helczynski, Leszek; Anand, Aseem; Peltola, Mari; Pettersson, Kim; Lilja, Hans; Bjartell, Anders

    2016-05-01

    Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions. © 2016 The Histochemical Society.

  11. Electron-neutral scattering cross sections for CO2: a complete and consistent set and an assessment of dissociation

    International Nuclear Information System (INIS)

    Grofulović, Marija; Alves, Luís L; Guerra, Vasco

    2016-01-01

    This work proposes a complete and consistent set of cross sections for electron collisions with carbon dioxide (CO 2 ) molecules to be published in the IST-Lisbon database with LXCat. The set is validated from the comparison between swarm parameters calculated using a two-term Boltzmann solver and the available experimental data. The importance of superelastic collisions with CO 2 (0 1 0) molecules at low values of the reduced electric field is discussed. Due to significant uncertainties, there are ongoing debates regarding the deconvolution of cross sections that describe generic energy losses at specific energy thresholds into cross sections that describe individual processes. An important example of these uncertainties is with the dissociation of CO 2 , for which the total electron impact dissociation cross section has not yet been unambiguously identified. The available dissociation cross sections are evaluated and discussed, and a strategy to obtain electron-impact dissociation rate coefficients is suggested. (paper)

  12. [Application of Immunohistochemistry and Immunofluorescence Staining in Detection of Phospholipase A2 Receptor on Paraffin Section of Renal Biopsy Tissue].

    Science.gov (United States)

    Dong, Hong-rui; Wang, Yan-yan; Wang, Guo-qin; Sun, Li-jun; Cheng, Hong; Chen, Yi-pu

    2015-10-01

    To evaluate the application of immunohistochemistry and fluorescence staining method in the detection of phospholipase A2 receptor (PLA2R) on paraffin section of renal biopsy tissue,and to find an accurate and fast method for the detection of PLA2R in renal tissue. The PLA2R of 193 cases were detected by immunohistochemical staining,and the antigen was repaired by the method of high pressure cooker (HPC) hot repair plus trypsin repair. The 193 samples including 139 cases of idiopathic membranous nephropathy (IMN), 15 cases of membranous lupus nephritis, 8 cases of hepatitis B virus associated membranous nephropathy, 18 cases of IgA nephropathy, and 13 cases of minimal change diseases. To compare the dyeing effects, 22 paraffin sections of renal biopsy tissue of IMN cases with positive PLA2R were stained by using 4 different. of antigen repairing,which included HPC hot repair, HPC hot repair plus trypsin repair, water bath heat repair, and water bath heat repair plus trypsin repair. To compare the dyeing effects, 15 paraffin sections of renal biopsy tissue of IMN cases with positive PLA2R were stained by using 3 different. of antigen repairing,which included water bath heat repair plus trypsin repair, protease K digestion repair, and pepsin digestion repair. In 193 cases, the positive rate of PLA2R in IMN cases was 90.6% (126/139), and the other 54 patients without IMN were negative. Twenty-two IMN patients were positive for PLA2R by using the HPC heat repair plus trypsin repaire or the water bath heat repair plus trypsin repair;while only a few cases of 22 IMN cases were positive by using the HPC hot repair alone or water bath heat repair alone. Fifteen IMN patients were positive for PLA2R by using water bath heat repair plus trypsin repair,protease K digestion repair,and pepsin digestion repair, but the distribution of positive deposits and the background were different. PLA2R immunohistochemical staining can effectively identify IMN and secondary MN. For

  13. RootAnalyzer: A Cross-Section Image Analysis Tool for Automated Characterization of Root Cells and Tissues.

    Directory of Open Access Journals (Sweden)

    Joshua Chopin

    Full Text Available The morphology of plant root anatomical features is a key factor in effective water and nutrient uptake. Existing techniques for phenotyping root anatomical traits are often based on manual or semi-automatic segmentation and annotation of microscopic images of root cross sections. In this article, we propose a fully automated tool, hereinafter referred to as RootAnalyzer, for efficiently extracting and analyzing anatomical traits from root-cross section images. Using a range of image processing techniques such as local thresholding and nearest neighbor identification, RootAnalyzer segments the plant root from the image's background, classifies and characterizes the cortex, stele, endodermis and epidermis, and subsequently produces statistics about the morphological properties of the root cells and tissues. We use RootAnalyzer to analyze 15 images of wheat plants and one maize plant image and evaluate its performance against manually-obtained ground truth data. The comparison shows that RootAnalyzer can fully characterize most root tissue regions with over 90% accuracy.

  14. Angle-resolving time-of-flight electron spectrometer for near-threshold precision measurements of differential cross sections of electron-impact excitation of atoms and molecules

    International Nuclear Information System (INIS)

    Lange, M.; Matsumoto, J.; Setiawan, A.; Panajotovic, R.; Harrison, J.; Lower, J. C. A.; Newman, D. S.; Mondal, S.; Buckman, S. J.

    2008-01-01

    This article presents a new type of low-energy crossed-beam electron spectrometer for measuring angular differential cross sections of electron-impact excitation of atomic and molecular targets. Designed for investigations at energies close to excitation thresholds, the spectrometer combines a pulsed electron beam with the time-of-flight technique to distinguish between scattering channels. A large-area, position-sensitive detector is used to offset the low average scattering rate resulting from the pulsing duty cycle, without sacrificing angular resolution. A total energy resolution better than 150 meV (full width at half maximum) at scattered energies of 0.5-3 eV is achieved by monochromating the electron beam prior to pulsing it. The results of a precision measurement of the differential cross section for electron-impact excitation of helium, at an energy of 22 eV, are used to assess the sensitivity and resolution of the spectrometer

  15. Eikonal calculation of electron-capture cross sections in collisions of H atoms with fast projectiles

    International Nuclear Information System (INIS)

    Ho, T.S.; Lieber, M.; Chan, F.T.

    1981-01-01

    We have employed the eikonal method to calculate the cross section for the capture of an electron into an arbitrary nl subshell in collisions between hydrogen atoms and fast projectiles. the projectiles were protons, C 6+ , O 8+ , and Fe 24+ . The energy ranges considered were 20--100 keV in the proton case, and 40--200 keV per nucleon in the other cases. These projectiles were selected because of their importance in fusion plasmas. For the highly charged case of Fe 24+ we found that our formulas, while exact, involved a high degree of cancellation and produced unreliable numerical results, so that a numerical integration of the penultimate formula was substituted. In the proton case agreement with recent experimental data is excellent

  16. Multiphoton effects in electron-ion scattering: A limitation of the cross-section treatment

    International Nuclear Information System (INIS)

    Torres Silva, H.; Sakanaka, P.H.; Braga, L.C.

    1991-07-01

    The differential cross-section for inelastic scattering in the presence of an intense laser field, when applied to the calculation of energy balance and heating by multiphoton process, is a problem which is not completely solved yet. One of the main difficulties is the calculation of the absorption coefficients α-bar for a monoenergetic beam of electrons scattered by a static potential. There are contradictory results shown by different authors. Here we have derived α-bar starting under the framework of quantum mechanics and then making the classical correspondence (h/2π → 0) according to the kinetic theory, and show that the absorption coefficient is always positive for all values of the particle incoming velocity, v-vector i . Furthermore, we show that in the calculation of α-bar we recover the Coulomb logarithm term. (author). 18 refs, 5 figs, 2 tabs

  17. Discrimination between basal cell carcinoma and hair follicles in skin tissue sections by Raman micro-spectroscopy

    Science.gov (United States)

    Larraona-Puy, M.; Ghita, A.; Zoladek, A.; Perkins, W.; Varma, S.; Leach, I. H.; Koloydenko, A. A.; Williams, H.; Notingher, I.

    2011-05-01

    Skin cancer is the most common human malignancy and basal cell carcinoma (BCC) represents approximately 80% of the non-melanoma cases. Current methods of treatment require histopathological evaluation of the tissues by qualified personnel. However, this method is subjective and in some cases BCC can be confused with other structures in healthy skin, including hair follicles. In this preliminary study, we investigated the potential of Raman micro-spectroscopy (RMS) to discriminate between hair follicles and BCC in skin tissue sections excised during Mohs micrographic surgery (MMS). Imaging and diagnosis of skin sections was automatically generated using ' a priori'-built spectral model based on LDA. This model had 90 ± 9% sensitivity and 85 ± 9% specificity for discrimination of BCC from dermis and epidermis. The model used selected Raman bands corresponding to the largest spectral differences between the Raman spectra of BCC and the normal skin regions, associated mainly with nucleic acids and collagen type I. Raman spectra corresponding to the epidermis regions of the hair follicles were found to be closer to those of healthy epidermis rather than BCC. Comparison between Raman spectral images and the gold standard haematoxylin and eosin (H&E) histopathology diagnosis showed good agreement. Some hair follicle regions were misclassified as BCC; regions corresponded mainly to the outermost layer of hair follicle (basal cells) which are expected to have higher nucleic acid concentration. This preliminary study shows the ability of RMS to distinguish between BCC and other tissue structures associated to healthy skin which can be confused with BCC due to their similar morphology.

  18. Comparison of two methods of preparation of tissue to study the internal anatomy of the delphacid Togosodes orizicolus with microscopy of electronic light

    International Nuclear Information System (INIS)

    Macaya-Lizano, A.V.; Pereira, R.; Espinoza, A.M.

    1997-01-01

    Two methods of embedding, sectioning and staining were developed to study the internal anatomy of delphacid plant hopper Tagosodes orizicolus, one of the most important plagues of rice in Latin America and the only vector of the white leaf tenuivirus (RHBV), using both light and electron microscopy. The paraffines-hematoxyline-eosin Y method allows color identification of tissues, for example purple for fat tissue, pink for muscles, yellow-brown for exocutile, while the resin-toluidine-blue method preserves better the ultrastructure but do not permit color identification. The information obtained by these procedures is complementary and the material can also be used for in situ studies by immuno microscopy, to assess the changes in cell ultrastructure and the localization and replication of the RHBV during its infection cycle in the insect vector. (author) [es

  19. Cross section measurements of the elastic electron - deuteron scattering at MAMI

    Energy Technology Data Exchange (ETDEWEB)

    Kohl, Yvonne [Universitaet Mainz, Institut fuer Kernphysik (Germany); Collaboration: A1-Collaboration

    2015-07-01

    The electromagnetic form factors of light nuclei provide a sensitive test of our understanding of nuclei. Because the deuteron has spin one, three form factors are needed to fully describe the electromagnetic structure of the deuteron. Especially the deuteron charge radius is a favourite observable to compare experiment and calculation. Recently, an extensive measurement campaign has been performed at MAMI (Mainzer Microtron) to determine the deuteron charge radius using elastic electron scattering - with the aim to halve the error compared to previous such experiments. The experiment took place at the 3-spectrometer facility of the A1-collaboration. Cross section measurements of the elastic electron-deuteron scattering have been performed for 180 different kinematic settings in the low momentum transfer region. From these, the charge form factor can precisely be determined. Fitting the form factor with an appropiate fit function, the radius can then be determined from the slope at zero momentum transfer. The determined radius could then be used as a counterweight to the value obtained from the advanced atomic Lamb shift measurements, thus providing additional insight to the proton radius puzzle.

  20. Density and field effect on electron-ion collision cross-sections in hot dense plasma

    International Nuclear Information System (INIS)

    Gaufridy de Dortan, F. de

    2003-03-01

    Collisional excitation cross-sections are essential for the modeling of the properties of non equilibrium plasmas. There has been a lot of work on electron impact excitation of isolated ions, but in dense plasmas, neighboring particles are expected to widely disturb these electron transitions in atoms. Plasma modeling through a radially perturbed potential has already been done but is not satisfactory as it does not account for levels degeneracy breaking and its consequences. Introduction of a quasistatic electric micro-field of neighboring ions allows us to break spherical symmetry. Our original theoretical study has given birth to a numerical code that accurately computes collisional strengths and rates (in the Distorted Waves approach) in atoms submitted to a realistic micro-field. Hydrogen- and helium-like aluminium is studied. Stark mixing widely increases rates of transitions from high l levels and forbidden transitions are field-enhanced by many orders of magnitude until they reach allowed ones. Eventually, we conduct an elementary stationary collisional radiative study to investigate field-enhancement effects on corresponding line shapes. In cases we study (aluminium, hydrogen- and helium-like) we find a relatively weak increase of K-shell line broadening

  1. 77 FR 71101 - Electronic Filing in the Copyright Office of Notices of Intention To Obtain a Section 115...

    Science.gov (United States)

    2012-11-29

    ... available at: http://www.copyright.gov/laws/rulemaking.html . SUPPLEMENTARY INFORMATION: Background Section... whether a Notice filed in the Copyright Office is sufficient as a matter of law under this section, that... LIBRARY OF CONGRESS Copyright Office 37 CFR Part 201 [Docket No. RM 2012-4] Electronic Filing in...

  2. Stability measurements of the electron gun for the KATRIN rear section

    Energy Technology Data Exchange (ETDEWEB)

    Ebenhoech, Sylvia [Karlsruhe Institute of Technology (KIT) (Germany). Institute for Technical Physics (ITEP), Tritium Laboratory Karlsruhe (TLK); Collaboration: KATRIN-Collaboration

    2016-07-01

    The aim of the KArlsruhe TRItium Neutrino (KATRIN) experiment is to determine the antineutrino mass with a sensitivity of 0.2 eV (90 % C.L.) by high-precision spectroscopy close to the tritium beta decay endpoint at 18.6 keV. To achieve the sensitivity aim both statistical and systematic uncertainties must be minimized. One contribution to the systematic error are uncertainties in the column density of the tritium source or in the source activity. In order to detect and monitor changes in the tritium source the Rear Section, which is integrated at the rear end of the KATRIN setup, is used. The Rear Section includes an angular selective electron gun (e-gun) based on the photoelectric effect. With the e-gun it will be possible e.g. to achieve a precise calibration of the column source density. To achieve the requirements on the e-gun a stable UV light source is essential to produce photoelectrons. The presentation focuses on the setup of the e-gun and intensity stability measurements of the UV light source setup.

  3. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Carbon dioxide

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Makoto [Gaseous Electronics Institute, Nagoya, Aichi (Japan)

    2003-04-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for carbon dioxide (CO{sub 2}). About 1,240 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1901 through 2000 for CO{sub 2}. Finally, author's comments for CO{sub 2} electron collision cross sections are given. (author)

  4. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Argon

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Makoto [Gaseous Electronics Institute, Nagoya, Aichi (Japan)

    2003-01-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for argon (Ar). About 1,960 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1921 through 2000 for Ar. Finally, author's recommended Ar electron collision cross section set is given in numerical tables. (author)

  5. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Sulphur hexafluoride

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Makoto [Gaseous Electronics Institute, Nagoya, Aichi (Japan)

    2003-05-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for sulphur hexafluoride (SF{sub 6}). About 920 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1934 through 2000 for SF{sub 6}. Finally, author's comments for SF{sub 6} electron collision cross section are given. (author)

  6. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Carbon dioxide

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-04-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for carbon dioxide (CO 2 ). About 1,240 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1901 through 2000 for CO 2 . Finally, author's comments for CO 2 electron collision cross sections are given. (author)

  7. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Halogen molecules

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-12-01

    A bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for halogen molecules (F 2 , Cl 2 , Br 2 , I 2 ). About 190(F 2 ), 360(Cl 2 ), 140(Br 2 ) and 240(I 2 ) papers were compiled respectively. A comprehensive author indexes for each molecule are included. The bibliography covers the period 1901 through 2000 for F 2 -I 2 . Finally, author's comments for F 2 -I 2 electron collision cross sections are given. (author)

  8. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Hydrogen halide molecules

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2004-03-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for hydrogen halide molecules (HF, HCL, HBr, HI). About 330 (HF), 420 (HCl) 220 (HBr) and 150 (HI) papers were compiled respectively. Comprehensive author indexes for each molecule are included. The bibliography covers the period 1903 through 2000 for HF-HI. Finally, author's comments for HBr electron collision cross sections are given. (author)

  9. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Water vapour

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-12-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for water vapour (H 2 O, D 2 O and HDO). About 1200 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1915 through 2000 for H 2 O. Finally, author's comments for electron collision cross sections and photodissociation processes of H 2 O are given. (author)

  10. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Hydrogen molecules

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2004-02-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for hydrogen molecules (H 2 , HD, HT, D 2 , DT, T 2 ). About 2200 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1901 through 2000 for H 2 . Finally, author's comments for H 2 electron collision cross sections are given. (author)

  11. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Nitrogen molecule

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-06-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for nitrogen molecule (N 2 ). About 2240 papers were compiled. A comprehensive author index is include. The bibliography covers the period 1906 through 2000 for N 2 . Finally, author's comments for N 2 electron collision cross section are given. (author)

  12. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Argon

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-01-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for argon (Ar). About 1,960 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1921 through 2000 for Ar. Finally, author's recommended Ar electron collision cross section set is given in numerical tables. (author)

  13. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Sulphur hexafluoride

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-05-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for sulphur hexafluoride (SF 6 ). About 920 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1934 through 2000 for SF 6 . Finally, author's comments for SF 6 electron collision cross section are given. (author)

  14. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Ammonia and phosphine

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2004-08-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for ammonia (NH 3 ) and phosphine (PH 3 ). About 820 (NH 3 ) and 190 (PH 3 ) papers were compiled respectively. Comprehensive author indexes for each molecule are included. The bibliography covers the period 1922 through 2000 for NH 3 and 1928 through 2000 for PH 3 . Finally, author's comments for NH 3 electron collision cross sections are given. (author)

  15. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Carbon dioxide

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Makoto [Gaseous Electronics Institute, Nagoya, Aichi (Japan)

    2003-04-01

    A bibliography of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for carbon dioxide (CO{sub 2}). About 1,240 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1901 through 2000 for CO{sub 2}. Finally, author's comments for CO{sub 2} electron collision cross sections are given. (author)

  16. Measurement of differential incoherent scattering cross-sections of 145 keV photons from K-shell electrons

    Energy Technology Data Exchange (ETDEWEB)

    Acharya, V B; Ghumman, B S [Punjabi Univ., Patiala (India). Dept. of Physics

    1980-06-01

    Differential cross-sections for incoherent scattering of 145 keV photons from K-shell electrons of tin, silver and molybdenum have been measured at 110deg to investigate the effect of electron binding on differential cross-sections in the low energy region. The incoherent scattered photons are selected in coincidence with X-rays which follow the vacancies caused by the ejection of the electrons. NaI(Tl) scintillators are used for the detection of scattered photons and emitted X-rays. The experimental results are compared with the available theoretical data.

  17. Cross-sections for dissociative excitation of lead atom in collisions of slow electrons with PbI2 molecules

    International Nuclear Information System (INIS)

    Smirnov, Yu.M.

    2006-01-01

    The dissociative excitation of the lead atom in e-PbI 2 collisions has been studied experimentally. 27 excitation cross-sections are measured at an exciting-electron energy of 100 eV. Nine optical excitation functions are recorded at the electron energy varying in the 0-100 eV range. The most possible reaction channels at low electron energies along with the relation of the dissociative-excitation cross-sections of the lead atom both in e-PbI 2 and e-PbCl 2 collisions are discussed. (authors)

  18. Bibliography of electron and photon cross sections with atoms and molecules published in the 20th century. Xenon

    International Nuclear Information System (INIS)

    Hayashi, Makoto

    2003-09-01

    A bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for xenon (Xe). About 1180 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1921 through 2000 for Xe. Finally, author's recommended Xe electron collision cross section set is given by number tables. (author)

  19. Bibliography of electron and photon cross sections with atoms and molecules. Published in the 20th century. Methane

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Makoto [Gaseous Electronics Inst., Sakae, Nagoya (Japan)

    2004-10-01

    Bibliographies of original and review reports of experiments or theories of electron and photon cross sections and also electron swarm data are presented for atomic or molecular species with specified targets. These works covered 17 atoms and 51 molecules. The present bibliography is only for methane (CH{sub 4}). About 1050 papers were compiled. A comprehensive author index is included. The bibliography covers the period 1924 through 2000 for CH{sub 4}. Finally, author's comments for CH{sub 4} electron collision cross sections are given. (author)

  20. HistoStitcher© : An Interactive Program for Accurate and Rapid Reconstruction of Digitized Whole Histological Sections from Tissue Fragments

    Science.gov (United States)

    Chappelow, Jonathan; Tomaszewski, John E.; Feldman, Michael; Shih, Natalie; Madabhushi, Anant

    2011-01-01

    We present an interactive program called HistoStitcher© for accurate and rapid reassembly of histology fragments into a pseudo-whole digitized histological section. HistoStitcher© provides both an intuitive graphical interface to assist the operator in performing the stitch of adjacent histology fragments by selecting pairs of anatomical landmarks, and a set of computational routines for determining and applying an optimal linear transformation to generate the stitched image. Reconstruction of whole histological sections from images of slides containing smaller fragments is required in applications where preparation of whole sections of large tissue specimens is not feasible or efficient, and such whole mounts are required to facilitate (a) disease annotation and (b) image registration with radiological images. Unlike manual reassembly of image fragments in a general purpose image editing program (such as Photoshop), HistoStitcher© provides memory efficient operation on high resolution digitized histology images and a highly flexible stitching process capable of producing more accurate results in less time. Further, by parameterizing the series of transformations determined by the stitching process, the stitching parameters can be saved, loaded at a later time, refined, or reapplied to multi-resolution scans, or quickly transmitted to another site. In this paper, we describe in detail the design of HistoStitcher© and the mathematical routines used for calculating the optimal image transformation, and demonstrate its operation for stitching high resolution histology quadrants of a prostate specimen to form a digitally reassembled whole histology section, for 8 different patient studies. To evaluate stitching quality, a 6 point scoring scheme, which assesses the alignment and continuity of anatomical structures important for disease annotation, is employed by three independent expert pathologists. For 6 studies compared with this scheme, reconstructed sections

  1. Total cross sections for electron scattering by CO2 molecules in the energy range 400 endash 5000 eV

    International Nuclear Information System (INIS)

    Garcia, G.; Manero, F.

    1996-01-01

    Total cross sections for electron scattering by CO 2 molecules in the energy range 400 endash 5000 eV have been measured with experimental errors of ∼3%. The present results have been compared with available experimental and theoretical data. The dependence of the total cross sections on electron energy shows an asymptotic behavior with increasing energies, in agreement with the Born-Bethe approximation. In addition, an analytical formula is provided to extrapolate total cross sections to higher energies. copyright 1996 The American Physical Society

  2. Absolute experimental cross sections for the ionization of singly charged barium ions by electron impact: Technical summary report

    International Nuclear Information System (INIS)

    Feeney, R.K.; Hooper, J.W.

    1971-01-01

    The absolute cross sections for the single ionization of Ba + ions by electron impact have been measured as a function of incident electron energy over the electron energy range from below threshold (10.001 eV) to approximately 1000 eV. It is found that the cross section increases from 1.94 x 10 -16 cm 2 to 3.76 x 10 -16 cm 2 between 15.5 and 18 eV actual incident electron energy. This rapid rise is interpreted as the onset of autoionization. Some evidence of structure occurring near the peak of the cross section curve such as found in the isoelectronic system of Cs is observed, but the relative magnitude of the apparent structure is of the same order as the 90% random error confidence limits and thus cannot conclusively be regarded as being present. 56 refs., 16 figs., 7 tabs

  3. A Survey of Electron Impact Cross-Sections for Halogens and Halogen Compounds of Interest to Plasma Processing

    Science.gov (United States)

    Sharma, S. P.; Rao, M. V. V. S.; Arnold, James O. (Technical Monitor)

    1998-01-01

    Published electron impact cross section data on halogens Cl2, F2, and halogen containing compounds such as Cx Fy, HCl, Cx Cly Fz are reviewed and critically evaluated based on the information provided by various researchers. The present work reports data on electron impact excitation, ionization, dissociation, electron attachment, electron detachment, and photo detachment. Elastic scattering cross sections and data on bulk properties such as diffusion coefficients in various background gases are also evaluated. Since some of the cross sectional data is derived from indirect measurements such as drift velocity, care has been taken to reconcile the differences among the reported data with due attention to the measurement technique. In conclusion, the processes with no or very limited amount of data and questionable set of data are identified and recommendation for further research direction is made.

  4. Engineered hybrid cardiac patches with multifunctional electronics for online monitoring and regulation of tissue function.

    Science.gov (United States)

    Feiner, Ron; Engel, Leeya; Fleischer, Sharon; Malki, Maayan; Gal, Idan; Shapira, Assaf; Shacham-Diamand, Yosi; Dvir, Tal

    2016-06-01

    In cardiac tissue engineering approaches to treat myocardial infarction, cardiac cells are seeded within three-dimensional porous scaffolds to create functional cardiac patches. However, current cardiac patches do not allow for online monitoring and reporting of engineered-tissue performance, and do not interfere to deliver signals for patch activation or to enable its integration with the host. Here, we report an engineered cardiac patch that integrates cardiac cells with flexible, freestanding electronics and a 3D nanocomposite scaffold. The patch exhibited robust electronic properties, enabling the recording of cellular electrical activities and the on-demand provision of electrical stimulation for synchronizing cell contraction. We also show that electroactive polymers containing biological factors can be deposited on designated electrodes to release drugs in the patch microenvironment on demand. We expect that the integration of complex electronics within cardiac patches will eventually provide therapeutic control and regulation of cardiac function.

  5. Electronic Health Record-Related Safety Concerns: A Cross-Sectional Survey of Electronic Health Record Users

    Science.gov (United States)

    Pajunen, Tuuli; Saranto, Kaija; Lehtonen, Lasse

    2016-01-01

    Background The rapid expansion in the use of electronic health records (EHR) has increased the number of medical errors originating in health information systems (HIS). The sociotechnical approach helps in understanding risks in the development, implementation, and use of EHR and health information technology (HIT) while accounting for complex interactions of technology within the health care system. Objective This study addresses two important questions: (1) “which of the common EHR error types are associated with perceived high- and extreme-risk severity ratings among EHR users?”, and (2) “which variables are associated with high- and extreme-risk severity ratings?” Methods This study was a quantitative, non-experimental, descriptive study of EHR users. We conducted a cross-sectional web-based questionnaire study at the largest hospital district in Finland. Statistical tests included the reliability of the summative scales tested with Cronbach’s alpha. Logistic regression served to assess the association of the independent variables to each of the eight risk factors examined. Results A total of 2864 eligible respondents provided the final data. Almost half of the respondents reported a high level of risk related to the error type “extended EHR unavailability”. The lowest overall risk level was associated with “selecting incorrectly from a list of items”. In multivariate analyses, profession and clinical unit proved to be the strongest predictors for high perceived risk. Physicians perceived risk levels to be the highest (Prisk levels (PeLearning courses on EHR-use was associated with lower risk for some of the risk factors. Conclusions Based on a large number of Finnish EHR users in hospitals, this study indicates that HIT safety hazards should be taken very seriously, particularly in operating rooms, procedure units, emergency departments, and intensive care units/critical care units. Health care organizations should use proactive and

  6. ZZ ELAST2, Database of Cross Sections for the Elastic Scattering of Electrons and Positrons by Atoms

    International Nuclear Information System (INIS)

    2002-01-01

    1 - Historical background and information: This database is an extension of the earlier database, 'Elastic Scattering of Electrons and Positrons by Atoms: Database ELAST', Report NISTIR 5188, 1993. Cross sections for the elastic scattering of electrons and positrons by atoms were calculated at energies from 1 KeV to 100 MeV. Up to 10 MeV the RELEL code of Riley was used. Above 10 MeV the ELSCAT code was used, which calculated the factored cross sections and evaluates the screening factor Kscr in WKB approximation. 2 - Application of the data: This database was developed to provide input for the transport codes, such as ETRAN, and includes differential cross sections, the total cross section, and the transport cross sections. In addition, a code TRANSX is provided that generates transport cross section of arbitrary order needed as input for the calculation of Goudsmit-Saunderson multiple-scattering angular distribution 3 - Source and scope of data: The database includes cross sections at 61 energies for electrons and 41 energies from positrons, covering the energy region from 1 KeV to 100 MeV. The number of deflection angles included in the database is 314 angles. Total and transport cross sections are also included in this package. The data files have an extension (jjj) that represents the atomic number of the target atom. The database includes auxiliary data files that enable the ELASTIC code to include the following optional modifications: (i) the inclusion of the exchange correction for electrons scattering; (ii) the conversion of the cross sections for scattering by free atoms to cross sections for scattering by atoms in solids; (iii) ti reduction of the cross sections at large angles and at high energies when the nucleus is treated as an extended rather than a point charge

  7. Measurement of charged current deep inelastic scattering cross sections with a longitudinally polarised electron beam at HERA

    International Nuclear Information System (INIS)

    Chekanov, S.; Derrick, M.; Magill, S.

    2008-12-01

    Measurements of the cross sections for charged current deep inelastic scattering in e - p collisions with longitudinally polarised electron beams are presented. The measurements are based on a data sample with an integrated luminosity of 175 pb -1 collected with the ZEUS detector at HERA at a centre-of-mass energy of 318 GeV. The total cross section is given for positively and negatively polarised electron beams. The differential cross-sections dσ/dQ 2 , dσ/dx and dσ/dy are presented for Q 2 >200 GeV 2 . The double-differential cross-section d 2 σ/dxdQ 2 is presented in the kinematic range 280 2 and 0.015< x<0.65. The measured cross sections are compared with the predictions of the Standard Model. (orig.)

  8. Inclusive and exclusive cross sections for multiple ionization by fast, highly charged ions in the independent-electron approximation

    International Nuclear Information System (INIS)

    Ben-Itzhak, I.; Gray, T.J.; Legg, J.C.; McGuire, J.H.

    1988-01-01

    Cross sections for the ionization of n of N electrons with equal single-electron ionization probability P are considered. When both N and the projectile charge q are large, the cross sections for single and double ionization are both found to be approximately linear in q at 1 MeVamu. The ratio of double-to-single-ionization cross sections is independent of q. Moreover, first-order perturbation theory for the single-electron ionization probability P, which varies as q 2 , is found to be applicable due to the damping of contributions with large P caused by factors of (1-P)/sup N/ - /sup n/. For large P there are differences between the inclusive probability P and the probability NP commonly used for a target with N electrons. Both of these probabilities differ significantly from the exclusive probability NP(1-P)/sup N/ -1 for the ionization of only one electron. For large N and large q, the exclusive ionization probabilities for removing exactly n of the N electrons tend to be concentrated in somewhat separate ranges of impact parameters b, defining impact-parameter ''windows.'' The windows which we obtain using the quantum-mechanical semiclassical-Coulomb-approximation (SCA) probabilities are similar to those using classical Monte Carlo calculations. Model calculations, based on analytic fits to the SCA probabilities, are used to obtain approximate analytic expressions for single- and double-ionization cross sections and for the impact-parameter windows

  9. Comparison between 3D conventional techniques, field-in-field and electronic tissue compensation for mantle fields planning

    International Nuclear Information System (INIS)

    Martins, Lais P.; Silva, Leonardo P.; Trindade, Cassia; Garcia, Paulo L.; Santos, Maira R.; Batista, Delano V.S.

    2012-01-01

    External radiotherapy treatment for Hodgkin's lymphoma over diaphragm region requires large radiation fields with protections applied to larynx, humerus head and lungs. The size and shape of the field, which covers different depths, make it difficult to distribute a homogeneous dose. Techniques such as field-in-field and electronic tissue compensation may be used to make dose homogeneous and compensate the obliquity from the tissue. Three types of planning were performed for diagnose of nodular sclerosis Hodgkin's lymphoma: one plan with two fields, AP-PA (AP plan), another with four fields field-in- field (FF plan), and a third one with two fields and electronic tissue compensation (ETC plan). Results showed better gradient, cover of PTV and dose distribution for the ETC plan, besides the advantage from this technique of does not require protection blocks. In the meanwhile, AP and FF plans require simpler dosimetry and fewer MU. Related to the uniformity of dose distribution, AP plan showed hot areas in the neck region, FF plan showed hot areas in the shoulder region and ETC plan showed most uniform distribution without hot areas. The electronic tissue compensation is a useful tool for large and shaped fields as the mantle field, however higher MU and complex dosimetry should be taken in account. (author)

  10. Comparison of the potentials used for the calculation of the resonant coherent electron capture and loss cross sections

    International Nuclear Information System (INIS)

    Pauly, N.; Dubus, A.; Roesler, M.

    2003-01-01

    For incident protons with velocities around 1 a.u., electron capture and loss processes are known to play an important role. In particular, electron emission as well as electronic stopping power are strongly influenced by the charge changing processes. Several different electron capture and loss processes have been identified [Solid State Phys. 43 (1990) 229], i.e. Auger valence band processes, shell processes and resonant coherent processes. In the latter, the incident projectile undergoes a periodic excitation due to the periodic crystalline potential so that an electron can be lost or captured. In the literature, several different choices have been made for the crystalline potential. It is precisely the aim of the present work to review and compare the various potentials used in the literature and to show the influence of this choice on the resonant coherent electron capture and loss cross sections

  11. Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation.

    Science.gov (United States)

    Goodwin, Richard J A; Nilsson, Anna; Borg, Daniel; Langridge-Smith, Pat R R; Harrison, David J; Mackay, C Logan; Iverson, Suzanne L; Andrén, Per E

    2012-08-30

    Analysis of whole animal tissue sections by MALDI MS imaging (MSI) requires effective sample collection and transfer methods to allow the highest quality of in situ analysis of small or hard to dissect tissues. We report on the use of double-sided adhesive conductive carbon tape during whole adult rat tissue sectioning of carboxymethyl cellulose (CMC) embedded animals, with samples mounted onto large format conductive glass and conductive plastic MALDI targets, enabling MSI analysis to be performed on both TOF and FT-ICR MALDI mass spectrometers. We show that mounting does not unduly affect small molecule MSI detection by analyzing tiotropium abundance and distribution in rat lung tissues, with direct on-tissue quantitation achieved. Significantly, we use the adhesive tape to provide support to embedded delicate heat-stabilized tissues, enabling sectioning and mounting to be performed that maintained tissue integrity on samples that had previously been impossible to adequately prepare section for MSI analysis. The mapping of larger peptidomic molecules was not hindered by tape mounting samples and we demonstrate this by mapping the distribution of PEP-19 in both native and heat-stabilized rat brains. Furthermore, we show that without heat stabilization PEP-19 degradation fragments can detected and identified directly by MALDI MSI analysis. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. A hybrid electron and photon IMRT planning technique that lowers normal tissue integral patient dose using standard hardware.

    Science.gov (United States)

    Rosca, Florin

    2012-06-01

    To present a mixed electron and photon IMRT planning technique using electron beams with an energy range of 6-22 MeV and standard hardware that minimizes integral dose to patients for targets as deep as 7.5 cm. Ten brain cases, two lung, a thyroid, an abdominal, and a parotid case were planned using two planning techniques: a photon-only IMRT (IMRT) versus a mixed modality treatment (E+IMRT) that includes an enface electron beam and a photon IMRT portion that ensures a uniform target coverage. The electron beam is delivered using a regular cutout placed in an electron cone. The electron energy was chosen to provide a good trade-off between minimizing integral dose and generating a uniform, deliverable plan. The authors choose electron energies that cover the deepest part of PTV with the 65%-70% isodose line. The normal tissue integral dose, the dose for ring structures around the PTV, and the volumes of the 75%, 50%, and 25% isosurfaces were used to compare the dose distributions generated by the two planning techniques. The normal tissue integral dose was lowered by about 20% by the E+IMRT plans compared to the photon-only IMRT ones for most studied cases. With the exception of lungs, the dose reduction associated to the E+IMRT plans was more pronounced further away from the target. The average dose ratio delivered to the 0-2 cm and the 2-4 cm ring structures for brain patients for the two planning techniques were 89.6% and 70.8%, respectively. The enhanced dose sparing away from the target for the brain patients can also be observed in the ratio of the 75%, 50%, and 25% isodose line volumes for the two techniques, which decreases from 85.5% to 72.6% and further to 65.1%, respectively. For lungs, the lateral electron beams used in the E+IMRT plans were perpendicular to the mostly anterior/posterior photon beams, generating much more conformal plans. The authors proved that even using the existing electron delivery hardware, a mixed electron/photon planning

  13. Reduction of dose enhancement from backscattered radiation at tissue-metal interfaces irradiated with 6MeV electrons

    International Nuclear Information System (INIS)

    Steel, B.

    1996-01-01

    Due to Electron Back Scatter (EBS), electron irradiation of tissue having under lying lead shielding results in an increase in dose to the tissue on the entrance side of the lead. In these situations dose increases as high as 80% have been reported in the literature. Saunders (British Journal of Radiology, 47, 467-470) noted that dose enhancement is dependent on atomic number of the under lying material approximately as Z 0.5 , and it increases at lower incident electron energies. In our clinic we use 2mm of lead shielding to protect under lying normal tissue when 6MeV electrons are used to treat lips and ears. The object of this study was to find the thinnest combination of materials to reduce the total dose to an acceptable level, with the provisos that; the patient does not come into contact with the lead or other metals, the finished shield could comfortabley be placed between the patient's lip and teeth, and that the materials are sufficietly malleable to work into custom shields. Various combinations of dental wax and aluminium were trialed. That which proved to give the best compromise between reduction of EBS and total shielding thickness was, 1mm of aluminim on the beam side of the lead with 1mm of dental wax to completely enclose the shield. In practice the manufactured shields are approximately 6 mm thick, and are usually not uncomfortable for the patient. (author)

  14. A Method for Preparing Spaceflight RNAlater-Fixed Arabidopsis thaliana (Brassicaceae Tissue for Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Eric R. Schultz

    2013-07-01

    Full Text Available Premise of the study: In spaceflight experiments, tissues for morphologic study are fixed in 3% glutaraldehyde, while tissues for molecular study are fixed in RNAlater; thus, an experiment containing both study components requires multiple fixation strategies. The possibility of using RNAlater-fixed materials for standard SEM-based morphometric investigation was explored to expand the library of tissues available for analysis and maximize usage of samples returned from spaceflight, but these technologies have wide application to any situation where recovery of biological resources is limited. Methods and Results: RNAlater-fixed samples were desalinated in distilled water, dehydrated through graded methanol, plunged into liquid ethane, and transferred to cryovials for freeze-substitution. Sample tissues were critical point dried, mounted, sputter-coated, and imaged. Conclusions: The protocol resulted in acceptable SEM images from RNAlater-fixed Arabidopsis thaliana tissue. The majority of the tissues remained intact, including general morphology and finer details such as root hairs and trichomes.

  15. Whole-brain serial-section electron microscopy in larval zebrafish.

    Science.gov (United States)

    Hildebrand, David Grant Colburn; Cicconet, Marcelo; Torres, Russel Miguel; Choi, Woohyuk; Quan, Tran Minh; Moon, Jungmin; Wetzel, Arthur Willis; Scott Champion, Andrew; Graham, Brett Jesse; Randlett, Owen; Plummer, George Scott; Portugues, Ruben; Bianco, Isaac Henry; Saalfeld, Stephan; Baden, Alexander David; Lillaney, Kunal; Burns, Randal; Vogelstein, Joshua Tzvi; Schier, Alexander Franz; Lee, Wei-Chung Allen; Jeong, Won-Ki; Lichtman, Jeff William; Engert, Florian

    2017-05-18

    High-resolution serial-section electron microscopy (ssEM) makes it possible to investigate the dense meshwork of axons, dendrites, and synapses that form neuronal circuits. However, the imaging scale required to comprehensively reconstruct these structures is more than ten orders of magnitude smaller than the spatial extents occupied by networks of interconnected neurons, some of which span nearly the entire brain. Difficulties in generating and handling data for large volumes at nanoscale resolution have thus restricted vertebrate studies to fragments of circuits. These efforts were recently transformed by advances in computing, sample handling, and imaging techniques, but high-resolution examination of entire brains remains a challenge. Here, we present ssEM data for the complete brain of a larval zebrafish (Danio rerio) at 5.5 days post-fertilization. Our approach utilizes multiple rounds of targeted imaging at different scales to reduce acquisition time and data management requirements. The resulting dataset can be analysed to reconstruct neuronal processes, permitting us to survey all myelinated axons (the projectome). These reconstructions enable precise investigations of neuronal morphology, which reveal remarkable bilateral symmetry in myelinated reticulospinal and lateral line afferent axons. We further set the stage for whole-brain structure-function comparisons by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. All obtained images and reconstructions are provided as an open-access resource.

  16. Accurate calculation of the differential cross section of compton scattering with electron mixed chain propagator in SM

    International Nuclear Information System (INIS)

    Chen Xuewen; Fang Zhenyun; Shi Chengye

    2012-01-01

    By using the electroweak standard model (SM), we analyzed the framework of electron mixed chain propagator which composed of serious of different physical loops participating in electroweak interaction and completed the relevant analytical calculation. Then, we obtained the analytical result of electron mixed chain propagator. By applying our result to Compton scattering, the differential cross section of Compton scattering dσ SM (chain) /dcosθ is counted accurately. This result is compared with the lowest order differential cross section dσ (tree) /dcosθ and the electronic chain propagator Compton scattering differential cross section dσ QED (chain) /dcosθ in quantum electrodynamics (QED). It can be seen that dσ SM (chain ) /dcosθ can show the radiation correction more subtly than dσ QED (chain) /dcosθ. (authors)

  17. Validity of the independent-processes approximation for resonance structures in electron-ion scattering cross sections

    International Nuclear Information System (INIS)

    Badnell, N.R.; Pindzola, M.S.; Griffin, D.C.

    1991-01-01

    The total inelastic cross section for electron-ion scattering may be found in the independent-processes approximation by adding the resonant cross section to the nonresonant background cross section. We study the validity of this approximation for electron excitation of multiply charged ions. The resonant-excitation cross section is calculated independently using distorted waves for various Li-like and Na-like ions using (N+1)-electron atomic-structure methods previously developed for the calculation of dielectronic-recombination cross sections. To check the effects of interference between the two scattering processes, we also carry out detailed close-coupling calculations for the same atomic ions using the R-matrix method. For low ionization stages, interference effects manifest themselves sometimes as strong window features in the close-coupling cross section, which are not present in the independent-processes cross section. For higher ionization stages, however, the resonance features found in the independent-processes approximation are found to be in good agreement with the close-coupling results

  18. Cross-section and rate coefficient calculation for electron impact excitation, ionisation and dissociation of H2 and OH molecules

    International Nuclear Information System (INIS)

    Riahi, R.; Ben Lakhdar, Z.; Teulet, Ph.; Gleizes, A.

    2006-01-01

    The weighted total cross-sections (WTCS) theory is used to calculate electron impact excitation, ionization and dissociation cross-sections and rate coefficients of OH, H 2 , OH + , H 2 + , OH - and H 2 - diatomic molecules in the temperature range 1500-15000 K. Calculations are performed for H 2 (X, B, C), OH(X, A, B), H 2 + (X), OH + (X, a, A, b, c), H 2 - (X) and OH - (X) electronic states for which Dunham coefficients are available. Rate coefficients are calculated from WTCS assuming Maxwellian energy distribution functions for electrons and heavy particles. One and 2 temperatures (θ e and θ g respectively for electron and heavy particles kinetic temperatures) results are presented and fitting parameters (a, b and c) are given for each reaction rate coefficient: k(θ) a(θ b )exp(-c/θ). (authors)

  19. Total cross sections for slow-electron (1--20 eV) scattering in solid H2O

    International Nuclear Information System (INIS)

    Michaud, M.; Sanche, L.

    1987-01-01

    An analytical method is proposed to determine absolute total cross sections per scatterer and related mean free paths for low-energy electron scattering in disordered molecular solid films. The procedure is based on a two-stream multiple-scattering model of the thickness dependence of the film reflectivity for elastic electrons. The expected analytical behavior and accuracy are tested on a model sample whose scattering properties are generated by a Monte Carlo simulation from initially known parameters. The effects of multiple scattering inside the film and at its interfaces are taken into account and discussed. The thickness dependence of the elastic electron reflectivity of H 2 O film condensed at 14 K is reported between 1 and 20 eV incident energy with a spectrometer resolution of 10 MeV. The proposed method is applied to extract from these measurements the energy dependence of the total effective and total inelastic cross sections for electron scattering in amorphous ice

  20. Determination of electron depth-dose curves for water, ICRU tissue, and PMMA and their application to radiation protection dosimetry

    International Nuclear Information System (INIS)

    Grosswendt, B.

    1994-01-01

    For monoenergetic electrons in the energy range between 60 keV and 10 MeV, normally incident on water, 4-element ICRU tissue and PMMA phantoms, depth-dose curves have been calculated using the Monte Carlo method. The phantoms' shape was that of a rectangular solid with a square front face of 30 cm x 30 cm and a thickness of 15 cm; it corresponds to that recommended by the ICRU for use in the procedure of calibrating radiation protection dosemeters. The depth-dose curves have been used to determine practical ranges, half-value depths, electron fluence to maximum absorbed dose conversion factors, and conversion factors between electron fluence and absorbed dose at depths d corresponding to 0.007 g.cm -2 , 0.3 g.cm -2 , and 1.0 g.cm -2 . The latter data can be used as fluence to dose equivalent conversion factors for extended parallel electron beams. (Author)

  1. Effective atomic numbers, electron densities, and tissue equivalence of some gases and mixtures for dosimetry of radiation detectors

    Directory of Open Access Journals (Sweden)

    Singh Vishwanath P.

    2012-01-01

    Full Text Available Total mass attenuation coefficients, µm, effective atomic number, Zeff, and effective electron density, Neff, of different gases - carbon dioxide, methane, acetylene, propane, butane, and pentane used in radiation detectors, have been calculated for the photon energy of 1 keV to 100 GeV. Each gas has constant Zeff values between 0.10 to 10 MeV photon energies; however, these values are way far away from ICRU tissue. Carbon dioxide gas shows the closest tissue equivalence in the entire photon energy spectrum. Relative tissue equivalences of the mixtures of gases with respect to ICRU tissue are in the range of 0.998-1.041 for air, argon (4.5% + methane (95.5%, argon (0.5% + carbon dioxide (99.5%, and nitrogen (5% + methane (7% + carbon dioxide (88%. The gas composition of xenon (0.5% + carbon dioxide (99.5% shows 1.605 times higher tissue equivalence compared to the ICRU tissue. The investigated photon interaction parameters are useful for exposure and energy absorption buildup factors calculation and design, and fabrication of gaseous detectors for ambient radiation measurement by the Geiger-Muller detector, ionization chambers and proportional counters.

  2. Laser resolution of unpolarized-electron scattering cross sections into spin-conserved and spin-flip components

    International Nuclear Information System (INIS)

    Ritchie, B.

    1981-01-01

    The theory is presented for one-photon free-free absorption by electrons scattering from high-Z atoms. The absorption cross section provides sufficient information to resolve the unpolarized-electron total cross section, Vertical Barf(theta)Vertical Bar 2 +Vertical Barg(theta)Vertical Bar 2 , into its individual components for spin-nonflip, Vertical Barf(theta)Vertical Bar 2 , and spin-flip, Vertical Barg(theta)Vertical Bar 2 , scattering. The observation of a spin-polarization effect for a spin-independent process (free-free absorption) is analogous to the Fano effect for bound-free absorption

  3. Electron stripping cross sections for light impurity ions in colliding with atomic hydrogens relevant to fusion research

    International Nuclear Information System (INIS)

    Tawara, H.

    1992-04-01

    Electron stripping (ionization) cross sections for impurity (carbon) ions with various charge states in collisions with atomic hydrogens have been surveyed. It has been found that these data are relatively limited both in collision energy and charge state and, in particular those necessary for high energy neutral beam injection (NBI) heating in fusion plasma research are scarce. Some relevant cross sections for carbon ions, C q+ (q = 0-5) have been estimated, based upon the existing data, empirical behavior and electron impact ionization data. (author)

  4. Measurement of the Charged-Current Quasi-Elastic Cross-Section for Electron Neutrinos on a Hydrocarbon Target

    Energy Technology Data Exchange (ETDEWEB)

    Wolcott, Jeremy [Univ. of Rochester, NY (United States)

    2016-01-01

    Appearance-type neutrino oscillation experiments, which observe the transition from muon neutrinos to electron neutrinos, promise to help answer some of the fundamental questions surrounding physics in the post-Standard-Model era. Because they wish to observe the interactions of electron neutrinos in their detectors, and because the power of current results is typically limited by their systematic uncertainties, these experiments require precise estimates of the cross-section for electron neutrino interactions. Of particular interest is the charged-current quasi-elastic (CCQE) process, which gures signi cantly in the composition of the reactions observed at the far detector. However, no experimental measurements of this crosssection currently exist for electron neutrinos; instead, current experiments typically work from the abundance of muon neutrino CCQE cross-section data and apply corrections from theoretical arguments to obtain a prediction for electron neutrinos. Veri cation of these predictions is challenging due to the di culty of constructing an electron neutrino beam, but the advent of modern high-intensity muon neutrino beams|together with the percent-level electron neutrino impurity inherent in these beams| nally presents the opportunity to make such a measurement. We report herein the rst-ever measurement of a cross-section for an exclusive state in electron neutrino scattering, which was made using the MINER A detector in the NuMI neutrino beam at Fermilab. We present the electron neutrino CCQE di erential cross-sections, which are averaged over neutrinos of energies 1-10 GeV (with mean energy of about 3 GeV), in terms of various kinematic variables: nal-state electron angle, nal-state electron energy, and the square of the fourmomentum transferred to the nucleus by the neutrino , Q2. We also provide a total cross-section vs. neutrino energy. While our measurement of this process is found to be in agreement with the predictions of the GENIE

  5. Experimental system to measure excitation cross-sections by electron impact. Measurements for ArI and ArII

    International Nuclear Information System (INIS)

    Blanco, F.; Sanchez, J.A.; Aguilera, J.A.; Campos, J.

    1989-01-01

    An experimental set-up to measure excitation cross-section of atomic and molecular levels by electron impact based on the optical method is reported. We also present some measurements on the excitation cross-section for ArI 5p'(1/2)0 level, and for simultaneous ionization and excitation of Ar leading to ArII levels belonging to the 3p 4 4p and 3p 4 4d configurations. (Author)

  6. Langmuir waves excitation by electron beam with the limited cross-section in the near-earth electron foreshock

    International Nuclear Information System (INIS)

    Anisimov, I.O.; Musatenko, K.S.; Krasnosselskikh, V.V.

    2005-01-01

    Model of the stripped monoenergetic electron beam with the sharp boundaries based on the results of CLUSTER measurements is proposed for the near-Earth foreshock vicinity. Dispersion equation is obtained and analyzed using numerical methods. Dependency of the equation roots corresponding to kinetic mechanism of beam-plasma instability on the model parameters is studied

  7. Dependence of radar auroral scattering cross section on the ambient electron density and the destabilizing electric field

    International Nuclear Information System (INIS)

    Haldoupis, C.; Nielsen, E.; Schlegel, K.

    1990-01-01

    By using a data set that includes simultaneous STARE and EISCAT measurements made at a common magnetic flux tube E region in the ionosphere, we investigate the dependence of relative scattering cross section of 1-meter auroral irregularities on the destabilizing E x B electron drift, or alternatively the electric field, and the E region ambient electron density. The analysis showed that both, the E field and mean electron density are the decisive factors in determining the strength of radar auroral echoes at magnetic aspect angles near perpendicularity. We have found that at instability threshold, i.e., when the E field strength is in the 15 to 20 mV/m range, the backscatter power level is affected strongly by the mean electron density. Above threshold, the wave saturation amplitudes are determined mainly by the combined action of electron drift velocity magnitude, V d , and mean electron density, N e , in a way that the scattering cross section, or the electron density fluctuation level, increases with electric field magnitude but at a rate which is larger when the ambient electron density is lower. The analysis enabled us to infer an empirical functional relationship which is capable of predicting reasonably well the intensity of STARE echoes from EISCAT E field and electron density data. In this functional relationship, the received power at threshold depends on N e 2 whereas, from threshold to perhaps more than 50 mV/m, the power increases nonlinearly with drift velocity as V d n where the exponent n is approximately proportional to N e -1/2 . The results support the Farley-Bunemann instability as the primary instability mechanism, but the existing nonlinear treatment of the theory, which includes wave-induced cross field diffusion, cannot account for the observed role of electron density in the saturation of irregularity amplitudes

  8. Electron capture cross sections by O{sup +} from atomic He

    Energy Technology Data Exchange (ETDEWEB)

    Joseph, Dwayne C; Saha, Bidhan C [Department of Physics, Florida A and M University, Tallahassee, FL-32307 (United States)

    2009-11-01

    The adiabatic representation is used in both the quantal and semi classical molecular orbital close coupling methods (MOCC) to evaluate charge exchange cross sections. Our results show good agreement with experimental cross sections

  9. Analysis of Chloroquine and Metabolites Directly from Whole-body Animal Tissue Sections by Liquid Extraction Surface Analysis (LESA) and Tandem Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Parson, Whitney B [ORNL; Koeniger, Stormy L [Abbott Laboratories; Johnson, Robert W [Abbott Laboratories; Erickson, Jamie [Abbott Laboratories; Tian, Yu [Abbott Laboratories; Stedman, Christopher A. [Abbott Laboratories; Schwartz, Annette [Abbott Laboratories; Tarcsa, Edit [Abbott Laboratories; Cole, Roderic [ORNL; Van Berkel, Gary J [ORNL

    2012-01-01

    The rapid and direct analysis of the amount and spatial distribution of exogenous chloroquine and chloroquine metabolites from tissue sections by liquid extraction surface sampling analysis coupled with tandem mass spectrometry (LESA-MS) was demonstrated. LESA-MS results compared well with previously published chloroquine quantification data collected by organ excision, extraction and fluorescent detection. The ability to directly sample and analyze spatially-resolved exogenous molecules from tissue sections with minimal sample preparation and analytical method development has the potential to facilitate the assessment of target tissue penetration of pharmaceutical compounds, to establish pharmacokinetic/pharmacodynamic (PK/PD) relationships, and to complement established pharmacokinetic methods used in the drug discovery process during tissue distribution assessment.

  10. Partial and total electronic stopping cross sections of atoms for a singly charged helium ion, Part 2

    International Nuclear Information System (INIS)

    Kaneko, T.; Nishikori, M.; Yamato, N.

    1991-08-01

    Partial and total electronic stopping cross sections of atoms with Z (55 ≤ Z ≤ 92) for a He + ion are tabulated as the second part of NIFS-DATA-11 (1991) on the basis of the wave-packet theory. (author)

  11. 77 FR 31237 - Electronic Filing in the Copyright Office of Notices of Intention To Obtain a Section 115...

    Science.gov (United States)

    2012-05-25

    ... law, such notices may be filed in the Office only when the public records of the Copyright Office do... filed in the Copyright Office is sufficient as a matter of law under this section, that issue shall be... LIBRARY OF CONGRESS Copyright Office 37 CFR Part 201 [Docket No. RM 2012-4] Electronic Filing in...

  12. Diagnostic accuracy of morphologic identification of filamentous fungi in paraffin embedded tissue sections: Correlation of histological and culture diagnosis

    Directory of Open Access Journals (Sweden)

    Sundaram Challa

    2014-01-01

    Full Text Available Aims and Objectives: The aim was to investigate the correlation between histological and culture diagnosis of filamentous fungi. Materials and Methods: Tissue sections from biopsy samples stained with Hematoxylin and Eosin and special stains from samples of chronic invasive/noninvasive sinusitis and intracranial space occupying lesions during 2005-2011 diagnosed to have infection due to filamentous fungi were reviewed. The histopathology and culture diagnoses were analyzed for correlation and discrepancy. Results: There were 125 samples positive for filamentous fungi on biopsy. Of these 76 (60.8% were submitted for culture and fungi grew in 30 (39.97% samples. There was a positive correlation between histological and culture diagnosis in 25 (83.33% samples that included Aspergillus species (16/19, Zygomycetes species (8/10 and dematiaceous fungi (1/1. The negative yield of fungi was more in Zygomycetes species (20/30 when compared to Aspergillus species (25/44. There was a discrepancy in diagnosis in 5/30 (16.67% samples which included probable dual infection in two, and dematiaceous fungi being interpreted as Aspergillus species in three samples. Conclusion: Histopathology plays a major role in the diagnosis of infection due to filamentous fungi, especially when cultures are not submitted or negative. The discrepancy between histological and culture diagnosis was either due to dematiaceous fungi being interpreted as Aspergillus species or probable dual infection.

  13. Identification of 5-hydroxytryptamine-producing cells by detection of fluorescence in paraffin-embedded tissue sections

    Directory of Open Access Journals (Sweden)

    Y. Kaneko

    2016-09-01

    Full Text Available 5-Hydroxytryptamine (5-HT produced by enterochromaffin (EC cells is an important enteric mucosal signaling ligand and has been implicated in several gastrointestinal diseases, including inflammatory bowel disease and functional disorders such as irritable bowel syndrome. The present study reports a new, simple and rapid visualization method of 5-HT-producing EC cells utilizing detection of autofluorescence in paraffin-embedded tissue sections after formalin fixation. In human samples, there was a high incidence of autofluorescence+ cells in the 5-HT+ cells in the pyloric, small intestinal and colonic glands, while co-localization was lacking between autofluorescence+ and gastrin+ cells in the pyloric and small intestinal glands. Autofluorescence+ EC cells were detected in the colon of mice and rats. Autofluorescence+ cells were also observed in 5-HT+ β cells in the pancreatic islets of Langerhans in pregnant mice, while non-pregnant mouse pancreatic islet cells showed no 5-HT immunoreactivity or autofluorescence. These results suggest that autofluorescence+ cells are identical to 5-HT+ cells, and the source of autofluorescence may be 5-HT itself or molecules related to its synthesis or degradation. This autofluorescence signal detection method may be applicable for monitoring of inflammatory status of inflammatory bowel diseases in both the experimental and clinical settings.

  14. Topology Optimization of an Actively Cooled Electronics Section for Downhole Tools

    DEFF Research Database (Denmark)

    Soprani, Stefano; Klaas Haertel, Jan Hendrik; Lazarov, Boyan Stefanov

    2015-01-01

    Active cooling systems represent a possible solution to the electronics overheating that occurs in wireline downhole tools operating in high temperature oil and gas wells. A Peltier cooler was chosen to maintain the downhole electronics to a tolerable temperature, but its integration into the dow......Active cooling systems represent a possible solution to the electronics overheating that occurs in wireline downhole tools operating in high temperature oil and gas wells. A Peltier cooler was chosen to maintain the downhole electronics to a tolerable temperature, but its integration......, according to the topology optimization results and assembly constraints, and compared to the optimized cases....

  15. Total electron scattering cross section from pyridine molecules in the energy range 10-1000 eV

    Science.gov (United States)

    Dubuis, A. Traoré; Costa, F.; da Silva, F. Ferreira; Limão-Vieira, P.; Oller, J. C.; Blanco, F.; García, G.

    2018-05-01

    We report on experimental total electron scattering cross-section (TCS) from pyridine (C5H5N) for incident electron energies between 10 and 1000 eV, with experimental uncertainties within 5-10%, as measured with a double electrostatic analyser apparatus. The experimental results are compared with our theoretical calculations performed within the independent atom model complemented with a screening corrected additivity rule (IAM-SCAR) procedure which has been updated by including interference effects. A good level of agreement is found between both data sources within the experimental uncertainties. The present TCS results for electron impact energy under study contribute, together with other scattering data available in the literature, to achieve a consistent set of cross section data for modelling purposes.

  16. Measurement of cross-sections for step-bystep excitation of inert gas atoms from metastable states by electron collisions

    International Nuclear Information System (INIS)

    Mityureva, A.A.; Penkin, N.P.; Smirnov, V.V.

    1989-01-01

    Excitation of argon atoms by electron collisions from metastable (MS) to high-lying states of inert gases (the so-called step-by-step excitation) is investigated. Formation of MS atoms m and their further step-by-step excitation up to k level is carried out by an electron beam with energy from 1 up to 40 eV. Time distribution of forming metastable and step-by-step electron collisions is used. The method used permits to measure the functions of step-by-step excitation and the absolute values of cross sections. Absolute values of cross-sections and functions of step-by-step excitation of some lines and argon levels are obtained

  17. Electron drift velocities in He and water mixtures: Measurements and an assessment of the water vapour cross-section sets

    International Nuclear Information System (INIS)

    Urquijo, J. de; Juárez, A. M.; Basurto, E.; Ness, K. F.; Robson, R. E.; White, R. D.; Brunger, M. J.

    2014-01-01

    The drift velocity of electrons in mixtures of gaseous water and helium is measured over the range of reduced electric fields 0.1–300 Td using a pulsed-Townsend technique. Admixtures of 1% and 2% water to helium are found to produce negative differential conductivity (NDC), despite NDC being absent from the pure gases. The measured drift velocities are used as a further discriminative assessment on the accuracy and completeness of a recently proposed set of electron-water vapour cross-sections [K. F. Ness, R. E. Robson, M. J. Brunger, and R. D. White, J. Chem. Phys. 136, 024318 (2012)]. A refinement of the momentum transfer cross-section for electron-water vapour scattering is presented, which ensures self-consistency with the measured drift velocities in mixtures with helium to within approximately 5% over the range of reduced fields considered

  18. Doubly differential cross sections of low-energy electrons emitted in the ionization of molecular hydrogen by bare carbon ions

    International Nuclear Information System (INIS)

    Tribedi, L.C.; Richard, P.; Ling, D.; Wang, Y.D.; Lin, C.D.; Moshammer, R.; Kerby, G.W. III; Gealy, M.W.; Rudd, M.E.

    1996-01-01

    We have measured the double differential cross sections (DDCS) (d 2 σ/d var-epsilon ed Ω e ) of low-energy electron emission in the ionization of H 2 bombarded by bare carbon ions of energy 30 MeV. The energy and angular distributions of the electron DDCS have been obtained for 12 different emission angles and for electron energies varying between 0.1 and 300 eV. We have also deduced the single differential and total ionization cross section from the measured DDCS. The data have been compared with the predictions of first Born approximations and the CDW-EIS (continuum distorted wave endash eikonal initial state) model. The CDW-EIS model provides an excellent agreement with the data. copyright 1996 The American Physical Society

  19. Polybrominated diphenyl ethers in chicken tissues and eggs from an electronic waste recycling area in southeast China

    Institute of Scientific and Technical Information of China (English)

    Xiaofei Qin; Yongjian Yang; Zhanfen Qin; Yan Li; Yaxian Zhao; Xijuan Xia; Shishuai Yan; Mi Tian; Xingru Zhao; Xiaobai XU

    2011-01-01

    The levels and distributions of polybrominated diphenyl ethers (PBDEs) in chicken tissues from an electronic waste (e-waste)recycling area in southeast China were investigated. Human dietary intake by local residents via chicken muscle and eggs was estimated.The mean PBDEs concentrations in tissues ranged from 15.2 to 3138.1 ng/g lipid weight (lw) and in egg the concentration was 563.5 ng/g lw. The results showed that the level of total PBDEs (∑PBDEs) in the chicken tissue was 2-3 orders of magnitude higher than those reported in the literature. The large difference of ΣPBDEs concentrations between tissues confirmed that the distribution of PBDEs in tissues depend on tissue-specificity rather than the “lipid-compartment”. BDE-209 was the predominant congener (82.5%-94.7% of ∑PBDEs) in all chicken tissues except in brain (34.7% of ∑PBDEs), which indicated that deca-BDE (the major commercial PBDE formulation comprising 65%-70% of total production) was major pollution source in this area and could be bioaccumulated in terrestrial animals. The dietary PBDEs intake of the local residents from chicken muscle and egg, assuming only local bred chickens and eggs were consumed, ranged from 2.2 to 22.5 ng/(day·kg body weight (bw)) with a mean value of 13.5 ng/(day.kg bw), which was one order of magnitude higher than the value reported in previous studies for consumption of all foodstuffs.

  20. Polybrominated diphenyl ethers in chicken tissues and eggs from an electronic waste recycling area in southeast China.

    Science.gov (United States)

    Qin, Xiaofei; Qin, Zhanfen; Li, Yan; Zhao, Yaxian; Xia, Xijuan; Yan, Shishuai; Tian, Mi; Zhao, Xingru; Xu, Xiaobai; Yang, Yongjian

    2011-01-01

    The levels and distributions of polybrominated diphenyl ethers (PBDEs) in chicken tissues from an electronic waste (e-waste) recycling area in southeast China were investigated. Human dietary intake by local residents via chicken muscle and eggs was estimated. The mean PBDEs concentrations in tissues ranged from 15.2 to 3138.1 ng/g lipid weight (lw) and in egg the concentration was 563.5 ng/g lw. The results showed that the level of total PBDEs (sigmaPBDEs) in the chicken tissue was 2-3 orders of magnitude higher than those reported in the literature. The large difference of sigmaPBDEs concentrations between tissues confirmed that the distribution of PBDEs in tissues depend on tissue-specificity rather than the "lipid-compartment". BDE-209 was the predominant congener (82.5%-94.7% of sigmaPBDEs) in all chicken tissues except in brain (34.7% of sigmaPBDEs), which indicated that deca-BDE (the major commercial PBDE formulation comprising 65%-70% of total production) was major pollution source in this area and could be bioaccumulated in terrestrial animals. The dietary PBDEs intake of the local residents from chicken muscle and egg, assuming only local bred chickens and eggs were consumed, ranged from 2.2 to 22.5 ng/(day x kg body weight (bw)) with a mean value of 13.5 ng/(day x kg bw), which was one order of magnitude higher than the value reported in previous studies for consumption of all foodstuffs.

  1. Radiobiological influence of megavoltage electron pulses of ultra-high pulse dose rate on normal tissue cells.

    Science.gov (United States)

    Laschinsky, Lydia; Karsch, Leonhard; Leßmann, Elisabeth; Oppelt, Melanie; Pawelke, Jörg; Richter, Christian; Schürer, Michael; Beyreuther, Elke

    2016-08-01

    Regarding the long-term goal to develop and establish laser-based particle accelerators for a future radiotherapeutic treatment of cancer, the radiobiological consequences of the characteristic short intense particle pulses with ultra-high peak dose rate, but low repetition rate of laser-driven beams have to be investigated. This work presents in vitro experiments performed at the radiation source ELBE (Electron Linac for beams with high Brilliance and low Emittance). This accelerator delivered 20-MeV electron pulses with ultra-high pulse dose rate of 10(10) Gy/min either at the low pulse frequency analogue to previous cell experiments with laser-driven electrons or at high frequency for minimizing the prolonged dose delivery and to perform comparison irradiation with a quasi-continuous electron beam analogue to a clinically used linear accelerator. The influence of the different electron beam pulse structures on the radiobiological response of the normal tissue cell line 184A1 and two primary fibroblasts was investigated regarding clonogenic survival and the number of DNA double-strand breaks that remain 24 h after irradiation. Thereby, no considerable differences in radiation response were revealed both for biological endpoints and for all probed cell cultures. These results provide evidence that the radiobiological effectiveness of the pulsed electron beams is not affected by the ultra-high pulse dose rates alone.

  2. Radiobiological influence of megavoltage electron pulses of ultra-high pulse dose rate on normal tissue cells

    International Nuclear Information System (INIS)

    Laschinsky, Lydia; Karsch, Leonhard; Schuerer, Michael; Lessmann, Elisabeth; Beyreuther, Elke; Oppelt, Melanie; Pawelke, Joerg; Richter, Christian

    2016-01-01

    Regarding the long-term goal to develop and establish laser-based particle accelerators for a future radiotherapeutic treatment of cancer, the radiobiological consequences of the characteristic short intense particle pulses with ultra-high peak dose rate, but low repetition rate of laser-driven beams have to be investigated. This work presents in vitro experiments performed at the radiation source ELBE (Electron Linac for beams with high Brilliance and low Emittance). This accelerator delivered 20-MeV electron pulses with ultra-high pulse dose rate of 10"1"0 Gy/min either at the low pulse frequency analogue to previous cell experiments with laser-driven electrons or at high frequency for minimizing the prolonged dose delivery and to perform comparison irradiation with a quasi-continuous electron beam analogue to a clinically used linear accelerator. The influence of the different electron beam pulse structures on the radiobiological response of the normal tissue cell line 184A1 and two primary fibroblasts was investigated regarding clonogenic survival and the number of DNA double-strand breaks that remain 24 h after irradiation. Thereby, no considerable differences in radiation response were revealed both for biological endpoints and for all probed cell cultures. These results provide evidence that the radiobiological effectiveness of the pulsed electron beams is not affected by the ultra-high pulse dose rates alone. (orig.)

  3. Electron impact single detachment on the F- ions using the heavy ion storage ring CRYRING: cross-section determination

    International Nuclear Information System (INIS)

    Andersson, K.; Hanstorp, D.; Oesterdahl, F.; Danared, H.; Kaellberg, A.

    2001-01-01

    Electron Impact Single Detachment (EISD) of F - has been studied using the heavy ion storage ring CRYRING at the Manne Siegbahn Laboratory, Stockholm, Sweden. F - ions stored in the ring were merged with an electron beam in one of the ring sections. Neutral F atoms produced in the EISD process were detected in the zero-degree direction using a surface barrier detector. The threshold for the detachment process was found to be around 7.6 eV, thus more than twice the binding energy of F - . The cross-sections increased smoothly up to 55 eV where it reached a maximum of 1.9 x 10 -16 cm 2 . At higher energies a slow decrease of the cross-section was observed, which follows the energy dependence predicted by the Bethe-Born approximation. The experiment showed that CRYRING can be used favourably for studies of anions, and several experiments are forthcoming. (orig.)

  4. Experimentation and correlates of electronic nicotine delivery system (electronic cigarettes) among university students – A cross sectional study

    OpenAIRE

    K.H. Awan

    2016-01-01

    Objective: E-cigarettes are becoming popular among youth as safe nicotine delivery systems. Many have expressed concern, however, that e-cigarettes may serve as a gateway to future smoking, given their low perceived risk, or that their use may prevent regular smokers from quitting by maintaining their nicotine addiction. The aim of this study was to assess experimentation with and correlates of e-cigarette use among university students. Material and methods: A cross-sectional study was car...

  5. Associations of attitudes towards electronic cigarettes with advertisement exposure and social determinants: a cross sectional study.

    Science.gov (United States)

    Reinhold, Benjamin; Fischbein, Rebecca; Bhamidipalli, Surya Sruthi; Bryant, Jennifer; Kenne, Deric R

    2017-01-01

    The exposure of young adults to electronic cigarette (e-cigarette) advertisements has risen rapidly. E-cigarette ads have been shown to increase short term perceived acceptability of using e-cigarettes in places where traditional cigarettes are banned. We set out to investigate if advertising exposure was related to perceptions of harm, addictiveness, and acceptability of use of e-cigarettes in places where traditional cigarettes are banned. Using a cross-sectional design, 6037 students at a large Midwestern university between the ages of 18-24 were surveyed about e-cigarette use and smoking status. Bivariate analyses were performed associating perception of harm, addictiveness, and acceptability of e-cigarette use in places where smoking is banned with demographic and other background factors, and e-cigarette advertising exposure through different media channels. Logistic regression analyses were used to explore the relationship of these factors on perceptions of harm, addictiveness and acceptability of e-cigarette use in places where smoking is banned. More than a quarter (27.4%) of respondents had used an e-cigarette, greater than half (53.2%) had seen an advertisement on TV and 42.0% had seen an advertisement on the Internet. Logistic regressions revealed that being white, male, an e-cigarette user, a smoker, having a mother who smoked, and Internet advertisement exposure were associated with lower perceived harm of e-cigarettes. The same factors, plus having seen advertisements on TV, were associated with increased likelihood of perceiving e-cigarette use in bars, stores, at work and in a dorm as acceptable. Perceiving use of e-cigarettes as acceptable in classrooms was also associated with the aforementioned factors and also included race. Only being male and an e-cigarette user were associated with lower perceived addictiveness of e-cigarettes. E-cigarette use is increasing in adolescents and young adults, as is exposure to e-cigarette advertising

  6. In situ hybridisation for identification and differentiation of Mycoplasma hyopneumoniae, Mycoplasma hyosynoviae and Mycoplasma hyorhinis in formalin-fixed porcine tissue sections

    DEFF Research Database (Denmark)

    Boye, Mette; Jensen, Tim Kåre; Ahrens, Peter

    2001-01-01

    Oligonucleotide probes targeting 16S ribosomal RNA were designed for species-specific identification of the porcine mycoplasmas Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma hyosynoviae using a fluorescent in situ hybridisation assay. The specificity of the probes was evaluated...... using pure cultures as well as porcine tissue sections with artificial presence of mycoplasma, and the probes were found specific for the target organisms. The assay was applied on sections of 28 tissue samples from pigs infected with one or more of the three Mycoplasma species as determined...

  7. Calculation of 3-D free electron laser gain: Comparison with simulation and generalization to elliptical cross section

    International Nuclear Information System (INIS)

    Chin, Yong Ho; Kim, Kwang-Je; Xie, Ming.

    1992-08-01

    In the previous paper, we have derived a dispersion relation for the free electron laser (FEL) gain in the exponential regime taking account the diffraction and electron's betatron oscillation. Here, we compare the growth rates obtained by solving the dispersion relation with those obtained by simulation calculation for the waterbag and the Gaussian models for the electron's transverse phase space distribution. The agreement is found to be good except for the limiting case where the Rayleigh length is much longer than the gain length (1-D limit). We also generalize the analysis to the case where the electron beam cross section is elliptical as is usually the case in storage rings, and derive the first-order dispersion relation

  8. Neutron cross section measurement using the Oak Ridge Electron Linear Accelerator

    International Nuclear Information System (INIS)

    Winters, R.R.

    1991-08-01

    This report discusses: argon-40 -- neutron reaction total cross sections from 6.9 kev to 50 kev; The maxwellian averaged neutron capture cross section of oxygen-16; r-matrix parameter analysis of the lead-208 -- neutron reaction cross section measurement; r-matrix parameter analysis of the ORELA neutron transmission zirconium-90 low energy measurement; porting computer codes from the HP9000 to the IBM RISC/6000;and measurements of neutron reactions with strontium-88, zirconium-90, and calcium-40

  9. Calculated sputtering and atomic displacement cross-sections for applications to medium voltage analytical electron microscopy

    International Nuclear Information System (INIS)

    Bradley, C.R.; Zaluzec, N.J.

    1987-08-01

    The development of medium voltage electron microscopes having high brightness electron sources and ultra-high vacuum environments has been anticipated by the microscopy community now for several years. The advantages of such a configuration have been discussed to great lengths, while the potential disadvantages have for the most part been neglected. The most detrimental of these relative to microcharacterization are the effects of electron sputtering and atomic displacement to the local specimen composition. These effects have in the past been considered mainly in the high voltage electron microscope regime and generally were ignored in lower voltage instruments. Recent experimental measurements have shown that the effects of electron sputtering as well as radiation induced segregation can be observed in conventional transmission electron microscopes. It is, therefore, important to determine at what point the effects will begin to manifest themselves in the new generation of medium voltage analytical electron microscopes. In this manuscript we present new calculations which allow the individual experimentalist to determine the potential threshold levels for a particular elemental system and thus avoid the dangers of introducing artifacts during microanalysis. 12 refs., 3 figs

  10. Ab initio calculation of scattering length and cross sections at very low energies for electron-helium scattering

    International Nuclear Information System (INIS)

    Saha, H.P.

    1993-01-01

    The multiconfiguration Hartree-Fock method for continuum wave functions has been used to calculate the scattering length and phase shifts over extremely low energies ranging from 0 to 1 eV very accurately for electron-helium scattering. The scattering length is calculated very accurately with wave functions computed exactly at zero energy, resulting in an upper bound of 1.1784. The electron correlation and polarization of the target by the scattering electron, which are very important in these calculations, have been taken into account in an accurate ab initio manner through the configuration-interaction procedure by optimizing both bound and continuum orbitals simultaneously at each kinetic energy of the scattered electron. Detailed results for scattering length, differential, total, and momentum-transfer cross sections obtained from the phase shifts are presented. The present scattering length is found to be in excellent agreement with the experimental result of Andrick and Bitsch [J. Phys. B 8, 402 (1975)] and the theoretical result of O'Malley, Burke, and Berrington [J. Phys. B 12, 953 (1979)]. There is excellent agreement between the present total cross sections and the corresponding experimental measurements of Buckman and Lohmann [J. Phys. B 19, 2547 (1986)]. The present momentum-transfer cross sections also show remarkable agreement with the experimental results of Crompton, Elford, and Robertson [Aust. J. Phys. 23, 667 (1970)

  11. Double ionization of the hydrogen sulfide molecule by electron impact: Influence of the target orientation on multiple differential cross sections

    Energy Technology Data Exchange (ETDEWEB)

    Imadouchene, N. [Laboratoire de Mécanique, Structures et Energétique Université Mouloud Mammeri de Tizi-Ouzou, B.P. 17, Tizi-Ouzou 15000 (Algeria); Aouchiche, H., E-mail: h_aouchiche@yahoo.fr [Laboratoire de Mécanique, Structures et Energétique Université Mouloud Mammeri de Tizi-Ouzou, B.P. 17, Tizi-Ouzou 15000 (Algeria); Champion, C. [Centre d’Etudes Nucléaires de Bordeaux Gradignan, Université Bordeaux, CNRS/IN2P3, Boîte Postale 120, Gradignan 33175 (France)

    2016-07-15

    Highlights: • The double ionization of the H{sub 2}S molecule is here theoretically studied. • The orientation dependence of the differential cross sections is scrutinized. • The specific double ionizing mechanisms are clearly identified. - Abstract: Multiple differential cross sections of double ionization of hydrogen sulfide molecule impacted by electrons are here investigated within the first Born approximation. In the initial state, the incident electron is represented by a plane wave function whereas the target is described by means of a single-center molecular wave function. In the final state, the two ejected electrons are described by Coulomb wave functions coupled by the Gamow factor, whereas the scattered electron is described by a plane wave. In this work, we analyze the role played by the molecular target orientation in the double ionization of the four outermost orbitals, namely 2b{sub 1}, 5a{sub 1}, 2b{sub 2} and 4a{sub 1} in considering the particular case of two electrons ejected from the same orbital. The contribution of each final state to the double ionization process is studied in terms of shape and magnitude for specific molecular orientations and for each molecular orbital we identified the mechanisms involved in the double ionization process, namely, the Shake-Off and the Two-Step 1.

  12. Cross section determination for the higher ionization of rare gas ions by electron collisions

    International Nuclear Information System (INIS)

    Becker, R.; Frodl, R.; Klein, H.; Schmidt, W.; Clausnitzer, G.; Klinger, H.; Mueller, A.; Salzborn, E.; Fuchs, G.; Viehboeck, F.

    1975-01-01

    The higher ionization of rare gas ions is reported on, which were excited by an electron beam using a crossed-beam technique. A detector for the identification of metastable excited rare gas ions was developed. (WL) [de

  13. Automatic cross-sectioning and monitoring system locates defects in electronic devices

    Science.gov (United States)

    Jacobs, G.; Slaughter, B.

    1971-01-01

    System consists of motorized grinding and lapping apparatus, sample holder, and electronic control circuit. Low power microscope examines device to pinpoint location of circuit defect, and monitor displays output signal when defect is located exactly.

  14. Electron Beam Induced Mass Loss Dependence on Stained Thin Epon Resin Sections

    Czech Academy of Sciences Publication Activity Database

    Skoupý, Radim; Nebesářová, Jana; Krzyžánek, Vladislav

    2016-01-01

    Roč. 22, S3 (2016), s. 926-927 ISSN 1431-9276 R&D Projects: GA ČR(CZ) GA14-20012S; GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 ; RVO:60077344 Keywords : TEM * STEM * EFTEM Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering; JA - Electronics ; Optoelectronics, Electrical Engineering (BC-A) Impact factor: 1.891, year: 2016

  15. Electron tomography of cryo-immobilized plant tissue: a novel approach to studying 3D macromolecular architecture of mature plant cell walls in situ.

    Directory of Open Access Journals (Sweden)

    Purbasha Sarkar

    Full Text Available Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The precise spatial three-dimensional (3D organization of cellulose, hemicellulose, pectin and lignin within plant cell walls remains unclear to date since the microscopy techniques used so far have been limited to two-dimensional, topographic or low-resolution imaging, or required isolation or chemical extraction of the cell walls. In this paper we demonstrate that by cryo-immobilizing fresh tissue, then either cryo-sectioning or freeze-substituting and resin embedding, followed by cryo- or room temperature (RT electron tomography, respectively, we can visualize previously unseen details of plant cell wall architecture in 3D, at macromolecular resolution (∼ 2 nm, and in near-native state. Qualitative and quantitative analyses showed that wall organization of cryo-immobilized samples were preserved remarkably better than conventionally prepared samples that suffer substantial extraction. Lignin-less primary cell walls were well preserved in both self-pressurized rapidly frozen (SPRF, cryo-sectioned samples as well as high-pressure frozen, freeze-substituted and resin embedded (HPF-FS-resin samples. Lignin-rich secondary cell walls appeared featureless in HPF-FS-resin sections presumably due to poor stain penetration, but their macromolecular features could be visualized in unprecedented details in our cryo-sections. While cryo-tomography of vitreous tissue sections is currently proving to be instrumental in developing 3D models of lignin-rich secondary cell walls, here we confirm that the technically easier method of RT-tomography of HPF-FS-resin sections could be used immediately for routine study of low-lignin cell walls. As a proof of principle, we

  16. A semi-empirical concept for the calculation of electron-impact ionization cross sections of neutral and ionized fullerenes

    International Nuclear Information System (INIS)

    Deutsch, H.; Scheier, P.; Maerk, T.D.; Becker, K.

    2002-01-01

    A semi-empirical approach to the calculation of cross section functions (absolute value and energy dependence) for the electron-impact ionization of several neutral and ionized fullerenes C 60 n+ (n =0-3) was developed, for which reliable experimental data have been reported. In particular, it is proposed a modification of the simplistic assumption that the ionization cross section of a cluster/fullerene is given as the product of the monomer ionization cross section and a factor m a , where 'm' is the number of monomers in the ensemble and 'a' is a constant. A comparison between these calculations and the available experimental data reveals good agreement for n = 0,103. In the case of ionization of C 60 2+ (n = 2) the calculation lies significantly below the measured cross section which it was interpret as an indication that additional indirect ionization processes are present for this charge state. (nevyjel)

  17. Electron-impact excitation cross sections of the higher argon 3p5np (n=5,6,7) levels

    International Nuclear Information System (INIS)

    Weber, Tobin; Boffard, John B.; Lin, Chun C.

    2003-01-01

    We have measured the electron-impact excitation cross sections for argon into the ten levels of 3p 5 5p configuration, as well as numerous levels of the 3p 5 6p and 3p 5 7p configurations. Fluorescence from the decay of the excited atoms to the levels of 3p 5 4s configuration was used to determine the optical-emission cross sections. These results were combined with transition probabilities to find apparent cross sections into the 3p 5 5p and 3p 5 6p levels. These new cross sections of the Ar(3p 5 np) levels along with the available Ne(2p 5 3p), Kr(4p 5 5p), and Xe(5p 5 6p) data help provide a global view of the excitation behaviors of the np 5 n ' p levels of the rare-gas series

  18. Comparison of drug distribution images from whole-body thin tissue sections obtained using desorption electrospray ionization tandem mass spectrometry and autoradiography.

    Science.gov (United States)

    Kertesz, Vilmos; Van Berkel, Gary J; Vavrek, Marissa; Koeplinger, Kenneth A; Schneider, Bradley B; Covey, Thomas R

    2008-07-01

    Desorption electrospray ionization tandem mass spectrometry (DESI-MS/MS) and whole-body autoradiography (WBA) were used for chemical imaging of whole-body thin tissue sections of mice intravenously dosed with propranolol (7.5 mg/kg). DESI-MS/MS imaging utilized selected reaction monitoring detection performed on an AB/MDS SCIEX 4000 QTRAP mass spectrometer equipped with a prototype extended length particle discriminator interface. Propranolol images of the tissue sections using DESI-MS/MS were obtained at surface scan rates of 0.1, 0.5, 2, and 7 mm/s. Although signal decreased with increasing scan rate, useful whole-body images for propranolol were obtained from the tissues even at 7 mm/s, which required just 79 min of analysis time. Attempts to detect and image the distribution of the known propranolol metabolites were unsuccessful. Regions of the tissue sections showing the most radioactivity from WBA sections were excised and analyzed by high-performance liquid chromatography (HPLC) with radiochemical detection to determine relative levels of propranolol and metabolites present. Comparison of the DESI-MS/MS signal for propranolol and the radioactivity attributed to propranolol from WBA sections indicated nominal agreement between the two techniques for the amount of propranolol in the brain, lung, and liver. Data from the kidney showed an unexplained disparity between the two techniques. The results of this study show the feasibility of using DESI-MS/MS to obtain useful chemical images of a drug in whole-body thin tissue sections following drug administration at a pharmacologically relevant level. Further optimization to improve sensitivity and enable detection of the drug metabolites will be among the requirements necessary to move DESI-MS/MS chemical imaging forward as a practical tool in drug discovery.

  19. Subshell differential cross sections for electron transfer in collisions of U{sup 90+} ions with gaseous targets

    Energy Technology Data Exchange (ETDEWEB)

    Ma, X. [Institute of Modern Physics, Lanzhou, GS (China); Stoehlker, T.; Bosch, F.; Brinzanescu, O.; Kozhuharov, C.; Mokler, P. [Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany); Fritzsche, S. [Kassel Univ. (Gesamthochschule) (Germany); Ludziejewski, T. [Institute of Nuclear Physics, Swierk (Poland); Warczak, A. [Uniwersytet Jagiellonski, Cracow (Poland). Inst. Fizyki

    2000-10-01

    Balmer spectra produced in collisions between He-like uranium projectiles and gaseous targets (N{sub 2}, Ar, Kr, and Xe) were measured at the jet-target area of the ESR storage ring. The X-ray radiation were recorded in coincidence with the down charged projectiles having captured one electron from the target atom. By means of spectra simulation, all the significant Balmer transition lines were identified. Further on, the j-differential cross sections for electron capture into projectile subshells were deduced from the well resolved spectra. For the heavier targets, where NRC contributes considerably, systematic deviations between experiment and the continuum distorted wave approximation are observed. (orig.)

  20. Transport coefficients and cross sections for electrons in water vapour: Comparison of cross section sets using an improved Boltzmann equation solution

    Science.gov (United States)

    Ness, K. F.; Robson, R. E.; Brunger, M. J.; White, R. D.

    2012-01-01

    This paper revisits the issues surrounding computation of electron transport properties in water vapour as a function of E/n0 (the ratio of the applied electric field to the water vapour number density) up to 1200 Td. We solve the Boltzmann equation using an improved version of the code of Ness and Robson [Phys. Rev. A 38, 1446 (1988)], facilitating the calculation of transport coefficients to a considerably higher degree of accuracy. This allows a correspondingly more discriminating test of the various electron-water vapour cross section sets proposed by a number of authors, which has become an important issue as such sets are now being applied to study electron driven processes in atmospheric phenomena [P. Thorn, L. Campbell, and M. Brunger, PMC Physics B 2, 1 (2009)] and in modeling charged particle tracks in matter [A. Munoz, F. Blanco, G. Garcia, P. A. Thorn, M. J. Brunger, J. P. Sullivan, and S. J. Buckman, Int. J. Mass Spectrom. 277, 175 (2008)].

  1. Detection of amyloid in abdominal fat pad aspirates in early amyloidosis: Role of electron microscopy and Congo red stained cell block sections

    Directory of Open Access Journals (Sweden)

    Sumana Devata

    2011-01-01

    Full Text Available Background: Fine-needle aspiration biopsy (FNA of the abdominal fat pad is a minimally invasive procedure to demonstrate tissue deposits of amyloid. However, protocols to evaluate amyloid in fat pad aspirates are not standardized, especially for detecting scant amyloid in early disease. Materials and Methods: We studied abdominal fat pad aspirates from 33 randomly selected patients in whom subsequent tissue biopsy, autopsy, and/or medical history for confirmation of amyloidosis (AL were also available. All these cases were suspected to have early AL, but had negative results on abdominal fat pad aspirates evaluated by polarizing microscopy of Congo Red stained sections (CRPM. The results with CRPM between four reviewers were compared in 12 cases for studying inter observer reproducibility. 24 cases were also evaluated by ultrastructural study with electron microscopy (EM. Results: Nine of thirty-three (27% cases reported negative by polarizing microscopy had amyloidosis. Reanalysis of 12 mixed positive-negative cases, showed considerable inter-observer variability with frequent lack of agreement between four observers by CRPM alone (Cohen′s Kappa index of 0.1, 95% CI -0.1 to 0.36. EM showed amyloid in the walls of small blood vessels in fibroadipose tissue in four out of nine cases (44% with amyloidosis. Conclusion: In addition to poor inter-observer reproducibility, CRPM alone in cases with scant amyloid led to frequent false negative results (9 out of 9, 100%. For improved detection of AL, routine ultrastructural evaluation with EM of fat pad aspirates by evaluating at least 15 small blood vessels in the aspirated fibroadipose tissue is recommended. Given the high false negative rate for CRPM alone in early disease, routine reflex evaluation with EM is highly recommended to avert the invasive option of biopsying various organs in cases with high clinical suspicion for AL.

  2. Measurement of charged current deep inelastic scattering cross sections with a longitudinally polarised electron beam at HERA

    Energy Technology Data Exchange (ETDEWEB)

    Chekanov, S.; Derrick, M.; Magill, S. [Argonne National Laboratory, IL (US)] (and others)

    2008-12-15

    Measurements of the cross sections for charged current deep inelastic scattering in e{sup -}p collisions with longitudinally polarised electron beams are presented. The measurements are based on a data sample with an integrated luminosity of 175 pb{sup -1} collected with the ZEUS detector at HERA at a centre-of-mass energy of 318 GeV. The total cross section is given for positively and negatively polarised electron beams. The differential cross-sections d{sigma}/dQ{sup 2}, d{sigma}/dx and d{sigma}/dy are presented for Q{sup 2}>200 GeV{sup 2}. The double-differential cross-section d{sup 2}{sigma}/dxdQ{sup 2} is presented in the kinematic range 280sections are compared with the predictions of the Standard Model. (orig.)

  3. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification

    DEFF Research Database (Denmark)

    Silahtaroglu, Asli; Nolting, Dorrit; Andersen, Lars Dyrskjøt

    2007-01-01

    been shown to increase detection sensitivity in FISH, combining these techniques into one protocol significantly decreases the time needed for miRNA detection in cryosections, while simultaneously retaining high detection sensitivity. Starting with fixation of the tissue sections, this miRNA FISH...

  4. INSITU BLOTTING - A NOVEL METHOD FOR DIRECT TRANSFER OF NATIVE PROTEINS FROM SECTIONED TISSUE TO BLOTTING MEMBRANE - PROCEDURE AND SOME APPLICATIONS

    NARCIS (Netherlands)

    OKABE, M; NYAKAS, C; BUWALDA, B; LUITEN, PGM

    We describe a novel technique for direct transfer of native proteins from unfixed frozen tissue sections to an immobilizing matrix, e.g., nitrocellulose, polyvinyliden difluoride, or positively charged nylon membranes. Proteins are directly blotted onto the membrane, providing optimal accessibility

  5. Bronchus-associated lymphoid tissue (BALT) lymphoma of the lung showing mosaic pattern of inhomogeneous attenuation on thin-section CT: a case report

    International Nuclear Information System (INIS)

    Lee, In Jae; Kim, Sung Hwan; Koo, Soo Hyun; Kim, Hyun Beom; Hwang, Dae Hyun; Lee, Kwan Seop; Lee, Yul; Jang, Kee Taek; Kim, Duck Hwan

    2000-01-01

    The authors present a case of histologically proven bronchus-associated lymphoid tissue (BALT) lymphoma of the lung in a patient with primary Sjogren's syndrome that manifested on thin-section CT scan as a mosaic pattern of inhomogeneous attenuation due to mixed small airway and infiltrative abnormalities

  6. Bronchus-associated lymphoid tissue (BALT) lymphoma of the lung showing mosaic pattern of inhomogeneous attenuation on thin-section CT: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, In Jae; Kim, Sung Hwan; Koo, Soo Hyun; Kim, Hyun Beom; Hwang, Dae Hyun; Lee, Kwan Seop; Lee, Yul; Jang, Kee Taek; Kim, Duck Hwan [Hallym University Sacred Heart Hospital, Anyang (Korea, Republic of)

    2000-09-01

    The authors present a case of histologically proven bronchus-associated lymphoid tissue (BALT) lymphoma of the lung in a patient with primary Sjogren's syndrome that manifested on thin-section CT scan as a mosaic pattern of inhomogeneous attenuation due to mixed small airway and infiltrative abnormalities.

  7. Gold internal standard correction for elemental imaging of soft tissue sections by LA-ICP-MS: element distribution in eye microstructures.

    Science.gov (United States)

    Konz, Ioana; Fernández, Beatriz; Fernández, M Luisa; Pereiro, Rosario; González, Héctor; Alvarez, Lydia; Coca-Prados, Miguel; Sanz-Medel, Alfredo

    2013-04-01

    Laser ablation coupled to inductively coupled plasma mass spectrometry has been developed for the elemental imaging of Mg, Fe and Cu distribution in histological tissue sections of fixed eyes, embedded in paraffin, from human donors (cadavers). This work presents the development of a novel internal standard correction methodology based on the deposition of a homogeneous thin gold film on the tissue surface and the use of the (197)Au(+) signal as internal standard. Sample preparation (tissue section thickness) and laser conditions were carefully optimized, and internal normalisation using (197)Au(+) was compared with (13)C(+) correction for imaging applications. (24)Mg(+), (56)Fe(+) and (63)Cu(+) distributions were investigated in histological sections of the anterior segment of the eye (including the iris, ciliary body, cornea and trabecular meshwork) and were shown to be heterogeneously distributed along those tissue structures. Reproducibility was assessed by imaging different human eye sections from the same donor and from ten different eyes from adult normal donors, which showed that similar spatial maps were obtained and therefore demonstrate the analytical potential of using (197)Au(+) as internal standard. The proposed analytical approach could offer a robust tool with great practical interest for clinical studies, e.g. to investigate trace element distribution of metals and their alterations in ocular diseases.

  8. Detection of Streptococcus suis by in situ hybridization, indirect immunofluorescence, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissue sections from pigs

    DEFF Research Database (Denmark)

    Boye, Mette; Feenstra, Anne Avlund; Tegtmeier, Conny

    2000-01-01

    and the immunohistochemical methods were used for detection of S. suis in formalin-fixed, paraffin-embedded tissue sections of brain, endocardium, and lung from pigs infected with S. suis. The methods developed were able to detect single cells of S. suis in situ in the respective samples, whereas no signal was observed from...

  9. Fast and Simple Protocols for Mass Spectrometry-Based Proteomics of Small Fresh Frozen Uterine Tissue Sections

    NARCIS (Netherlands)

    Dapic, I.; Uwugiaren, N.; Jansen, P.J.; Corthals, G.L.

    2017-01-01

    Human tissues are an important link between organ-specific spatial molecular information, patient pathology, and patient treatment options. However, patient tissues are uniquely obtained by time and location, and limited in their availability and size. Currently, little knowledge exists about

  10. Total projectile electron loss cross sections of U^{28+} ions in collisions with gaseous targets ranging from hydrogen to krypton

    Directory of Open Access Journals (Sweden)

    G. Weber

    2015-03-01

    Full Text Available Beam lifetimes of stored U^{28+} ions with kinetic energies of 30 and 50  MeV/u, respectively, were measured in the experimental storage ring of the GSI accelerator facility. By using the internal gas target station of the experimental storage ring, it was possible to obtain total projectile electron loss cross sections for collisions with several gaseous targets ranging from hydrogen to krypton from the beam lifetime data. The resulting experimental cross sections are compared to predictions by two theoretical approaches, namely the CTMC method and a combination of the DEPOSIT code and the RICODE program.

  11. Wakefield excitation by a sequence of relativistic electron bunches in dielectric waveguides of rectangular cross-section of various configurations

    International Nuclear Information System (INIS)

    Kiselev, V.A.; Linnik, A.F.; Mirnyj, V.I.; Onishchenko, I.N.; Sotnikov, G.V.; Uskov, V.V.

    2008-01-01

    The possibility to enhance the efficiency of wake wave excitation in dielectric waveguides of rectangular cross-section was investigated by increase of electron bunches coupling with excited wakefield that was achieved by decrease of transit channel cross-section. At that for each configuration the required changes of dielectric plates size were made to for maintain the coincidence concurrence of bunch repetition frequency and frequency of the principal transverse mode of the corresponding dielectric waveguide. It is established, the decrease of transit channel leading to essential changing of topography of total field excited wake wave

  12. Level-resolved distorted-wave cross-sections of electron impact ionization of Ar{sup 5+}

    Energy Technology Data Exchange (ETDEWEB)

    Yumak, A; Yavuz, I; Altun, Z, E-mail: zikalt@superonline.co [Department of Physics, Marmara University, Istanbul, 34722 (Turkey)

    2009-11-01

    Electron impact ionization cross sections of Ar{sup 5+} were calculated using configuration-average (CADW) and level-resolved (LRDW) distorted-wave methods. Direct ionization cross-sections of 2s, 2p, 3s and 3p subshells were calculated within a CADW approximation. The contributions from the excitation autoionization channels were evaluated assuming single excitations from the 2s, 2p, and 3s subshells and in both CADW and LRDW methods. The radiative stabilization of the excitation autoionization channels were found to reduce the excitation autoionization by a small amount.

  13. Cross section of W and Z inclusive production and the W/Z cross section ratio in association with one jet for the boson decaying into electron(s) with ATLAS 7 TeV data

    CERN Document Server

    Berglund, Elina

    2011-01-01

    This thesis investigates the inclusive production cross section of W and Z bosons as well as their ratio in association with one jet. The cross sections are estimated with 7 TeV LHC proton-proton collision data, collected by ATLAS during 2010, resulting in an integrated luminosity of approximately 40 pb−1 . This thesis focuses on the analysis performed in the electron channel, while comparisons with the results obtained in the muon channel also are included. This is the first time the W and Z production cross sections are measured at this center-of-mass energy and the results are important for various reasons, such as testing perturbative theoretical calculations and understanding these signatures as backgrounds to other physics processes. The final inclusive cross section values obtained in the electron channel are the following: σ(W → eν ) = 10.255 ± 0.031(stat) ± 0.190(syst) ± 0.349(lumi) ± 0.156(acc) nb σ(Z → ee) = 0.952 ± 0.010(stat) ± 0.026(syst) ± 0.032(lumi) ± 0.019(acc) nb The res...

  14. Secondary Electron Yield Measurements and Groove Chambers Tests in the PEP-II Beam Line Straights Sections

    International Nuclear Information System (INIS)

    Pivi, M

    2008-01-01

    Beam instability caused by the electron cloud has been observed in positron and proton storage rings and it is expected to be a limiting factor in the performance of the positron Damping Ring (DR) of future Linear Colliders such as ILC and CLIC [1, 2]. In the Positron Low Energy Ring (LER) of the PEP-II accelerator, we have installed vacuum chambers with rectangular grooves in a straight magnetic-free section to test this promising possible electron cloud mitigation technique. We have also installed a special chamber to monitor the secondary electron yield of TiN and TiZrV (NEG) coating, Copper, Stainless Steel and Aluminum under the effect of electron and photon conditioning in situ in the beam line. In this paper, we describe the ongoing R and D effort to mitigate the electron cloud effect for the ILC damping ring, the latest results on in situ secondary electron yield conditioning and recent update on the groove tests in PEP-II

  15. Electron Impact Excitation Cross Sections of Xenon for Optical Plasma Diagnostic

    National Research Council Canada - National Science Library

    Srivastava, Rajesh

    2007-01-01

    In this project the researcher had taken up the calculation of xenon apparent emission-excitation cross sections for emission lines that have diagnostic value in the analysis of Xe-propelled electric thruster plasmas...

  16. A new time of flight mass spectrometer for absolute dissociative electron attachment cross-section measurements in gas phase

    Science.gov (United States)

    Chakraborty, Dipayan; Nag, Pamir; Nandi, Dhananjay

    2018-02-01

    A new time of flight mass spectrometer (TOFMS) has been developed to study the absolute dissociative electron attachment (DEA) cross section using a relative flow technique of a wide variety of molecules in gas phase, ranging from simple diatomic to complex biomolecules. Unlike the Wiley-McLaren type TOFMS, here the total ion collection condition has been achieved without compromising the mass resolution by introducing a field free drift region after the lensing arrangement. The field free interaction region is provided for low energy electron molecule collision studies. The spectrometer can be used to study a wide range of masses (H- ion to few hundreds atomic mass unit). The mass resolution capability of the spectrometer has been checked experimentally by measuring the mass spectra of fragment anions arising from DEA to methanol. Overall performance of the spectrometer has been tested by measuring the absolute DEA cross section of the ground state SO2 molecule, and the results are satisfactory.

  17. Cross-sections of charge and electronic states change of particles at ion-ion and ion-molecule collisions

    International Nuclear Information System (INIS)

    Panov, M.N.; Afrosimov, V.V.; Basalaev, A.A.; Guschina, N.A.; Nikulin, V.K.

    2006-01-01

    The interactions of protons and alpha-particles with hydrocarbons are investigated. A quantum-mechanical computation of the electronic structure of all hydrocarbons from methane to butane and its fragment ions was performed in the Hartree-Fock RHF/UHF approximation using a GAMESS program (General Atomic Molecular Electron Structure System). The correlation energy was taken into account within the framework of MP2 perturbation theory. The structural parameters of the hydrocarbon molecules and their charged and neutral fragments were calculated in two cases: in the geometry of the parent molecule or of the relaxation states. The difference of the full energy of the same fragments in and out of brackets gives us the vibration excitation energies of the fragments at the moment of creation. Additional Mulliken effective charges (in electron charge units) of atoms in the fragments have been calculated. The calculations show that removing one electron from the ethane molecule without electronic excitation produced a single charged molecular ion in vibration state with binding energy of hydrogen atoms, some decimal eV. As results we obtain C 2 H 6 + and C 2 H 5 + . Additional fragmentation of hydrocarbon needs electronic excitation of produced single charged ions. Cross sections for electron capture and excitation processes in collisions between the hydrogen-like He + , B 4+ and O 7+ ions have been evaluated. The purpose of the theory within this project during the period under review was to get for the first time new data on Single-Electron Capture (SEC) and Excitation Processes (EP) in collisions of He + (1s) ions with hydrogen-like impurity ions B 4+ (1s) and O 7+ (1s) in the energy range for He + ions from 0.2 MeV to 3.0 MeV. The calculations were carried out by using the method of close-coupling equations with basis sets of eleven and ten quasimolecular two-electron states for reactions (1, 2) and (3, 4), respectively (entrance channel, seven charge transfer channels

  18. A comparison of the relative biological effectiveness of low energy electronic brachytherapy sources in breast tissue: a Monte Carlo study.

    Science.gov (United States)

    White, Shane A; Reniers, Brigitte; de Jong, Evelyn E C; Rusch, Thomas; Verhaegen, Frank

    2016-01-07

    Electronic brachytherapy sources use low energy photons to treat the tumor bed during or after breast-conserving surgery. The relative biological effectiveness of two electronic brachytherapy sources was explored to determine if spectral differences due to source design influenced radiation quality and if radiation quality decreased with distance in the breast. The RBE was calculated through the number of DNA double strand breaks (RBEDSB) using the Monte Carlo damage simulator (MCDS) in combination with other Monte Carlo electron/photon spectrum calculations. 50kVp photons from the Intrabeam (Carl Zeiss Surgical) and Axxent (Xoft) through 40-mm spherical applicators were simulated to account for applicator and tissue attenuation in a variety of breast tissue compositions. 40kVp Axxent photons were also simulated. Secondary electrons (known to be responsible for most DNA damage) spectra at different distance were inputted into MCDS to calculate the RBEDSB. All RBEDSB used a cobalt-60 reference. RBEDSB data was combined with corresponding average photon spectrum energy for the Axxent and applied to model-based average photon energy distributions to produce an RBEDSB map of an accelerated partial breast irradiation (APBI) patient. Both Axxent and Intrabeam 50kVp spectra were shown to have a comparable RBEDSB of between 1.4 and 1.6 at all distances in spite of progressive beam hardening. The Axxent 40kVp also demonstrated a similar RBEDSB at distances. Most RBEDSB variability was dependent on the tissue type as was seen in rib (RBEDSB  ≈  1.4), gland (≈1.55), adipose (≈1.59), skin (≈1.52) and lung (≈1.50). RBEDSB variability between both sources was within 2%. A correlation was shown between RBEDSB and average photon energy and used to produce an RBEDSB map of a dose distribution in an APBI patient dataset. Radiation quality is very similar between electronic brachytherapy sources studied. No significant reductions in RBEDSB were observed with

  19. Experimentation and correlates of electronic nicotine delivery system (electronic cigarettes) among university students - A cross sectional study.

    Science.gov (United States)

    Awan, K H

    2016-04-01

    E-cigarettes are becoming popular among youth as safe nicotine delivery systems. Many have expressed concern, however, that e-cigarettes may serve as a gateway to future smoking, given their low perceived risk, or that their use may prevent regular smokers from quitting by maintaining their nicotine addiction. The aim of this study was to assess experimentation with and correlates of e-cigarette use among university students. A cross-sectional study was carried out among 480 university students from four faculties at a university in Riyadh in August-October 2014. A modified version of the World Health Organization's Global Adult Tobacco Survey was used, and multinomial logistic regression was carried out to assess correlations with e-cigarette variables in the whole study sample and among smokers. Almost all students, including the majority of ex-smokers (96.3%) and smokers (94.4%), reported having heard about e-cigarettes. In addition, about one-quarter of the sample (54.2% of smokers, 24.7% of ex-smokers, 6% of never smokers) had experimented with e-cigarettes at least once during their lifetime. Curiosity and peer influence were reported as the main reasons for the use of e-cigarettes. Factors found to be correlated significantly with e-cigarette use were male gender, being a traditional cigarette smoker, having friends who have tried e-cigarettes, and having a strong belief that e-cigarettes could aid smoking cessation. E-cigarettes are popular among Saudi youth, especially among smokers and ex-smokers. Well-designed health education programs and regulatory interventions are required to address this issue.

  20. Differential N-glycan patterns identified in lung adenocarcinoma by N-glycan profiling of formalin-fixed paraffin-embedded (FFPE) tissue sections.

    Science.gov (United States)

    Wang, Xiaoning; Deng, Zaian; Huang, Chuncui; Zhu, Tong; Lou, Jiatao; Wang, Lin; Li, Yan

    2018-02-10

    N-glycan profiling is a powerful approach for analyzing the functional relationship between N-glycosylation and cancer. Current methods rely on either serum or fresh tissue samples; however, N-glycan patterns may differ between serum and tissue, as the proteins of serum originate from a variety of tissues. Furthermore, fresh tissue samples are difficult to ship and store. Here, we used a profiling method based on formalin-fixed paraffin-embedded (FFPE) tissue sections from lung adenocarcinoma patients. We found that our method was highly reproducible. We identified 58 N-glycan compositions from lung adenocarcinoma FFPE samples, 51 of which were further used for MS n -based structure prediction. We show that high mannose type N-glycans are upregulated, while sialylated N-glycans are downregulated in our FFPE lung adenocarcinoma samples, compared to the control samples. Our receiver operating characteristic (ROC) curve analysis shows that high mannose type and sialylated N-glycans are useful discriminators to distinguish between lung adenocarcinoma and control tissue. Together, our results indicate that expression levels of specific N-glycans correlate well with lung adenocarcinoma, and strongly suggest that our FFPE-based method will be useful for N-glycan profiling of cancer tissues. Glycosylation is one of the most important post-translational protein modifications, and is associated with several physiopathological processes, including carcinogenesis. In this study, we tested the feasibility of using formalin-fixed paraffin-embedded (FFPE) tissue sections to identify changes in N-glycan patterns and identified the differentially expressed N-glycans of lung adenocarcinoma. Our study shows that the FFPE-based N-glycan profiling method is useful for clinical diagnosis as well as identification of potential biomarkers, and our data expand current knowledge of differential N-glycan patterns of lung adenocarcinoma. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Imaging of early conifer embryogenic tissues with the environmental scanning electron microscope

    Czech Academy of Sciences Publication Activity Database

    Neděla, Vilém; Hřib, J.; Vooková, B.

    2012-01-01

    Roč. 56, č. 3 (2012), s. 595-598 ISSN 0006-3134 R&D Projects: GA ČR GAP102/10/1410 Institutional support: RVO:68081731 Keywords : Abies alba * A. numidica * extracellular matrix Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.692, year: 2012

  2. Calculation of the positronium formation differential cross section for collision of electron with anti-hydrogen atoms

    International Nuclear Information System (INIS)

    Ghanbari Adivi, E.; Kanjuri, F.; Bolorizadeh, M.

    2006-01-01

    The positronium formation differential cross sections in collision of the high-energy but non-relativistic electrons with anti-hydrogen atoms are calculated by using the three-body Faddeev-Watson-Lovelace formalism. In a second-order approximation, the inter-nuclear and nuclear-electronic partial amplitudes therein the Faddeev-Watson series are calculated, analytically, in the range of 0-180 degrees of the scattering angles. The presence of the T homas peak a t 45 d egree i s investigated. The results are discussed for 1 and 10 keV impact energies and for electron transition from anti-hydrogen ground state into the different states therein the K-, L- and M- shells of the positronium atoms.

  3. Ionization of liquid water by fast electron impact: multiple differential cross sections for the 1B1 orbital

    International Nuclear Information System (INIS)

    Fojon, O A; De Sanctis, M L; Stia, C R; Vuilleumier, R; Politis, M-F

    2011-01-01

    We present a theoretical study of single ionization of water molecules in liquid phase by impact of fast electrons in a coplanar geometry. Multiple differential cross sections are obtained through a first order model obtained within the framework of an independent electron approximation in which relaxation of the target is not taken into account. The wavefunctions for a single water molecule in the liquid phase are obtained through a Wannier orbital formalism and the ejected electron is described by means of Coulomb functions. We also present averaged calculations over all molecular orientations. A comparison with previous theoretical and experimental results, the latter corresponding to water in gaz phase, shows a good agreement. The main physical features of the reaction (such as binary and recoil peaks) present in measurements for vapor are also observed in the present theoretical predictions.

  4. Newly calculated absolute cross-section for the electron-impact ionization of C2H2+

    International Nuclear Information System (INIS)

    Deutsch, H.; Becker, K.; Defrance, P.; Probst, M.; Mark, T.D.; Limtrakul, J.

    2006-01-01

    New measurements of the cross-section for electron impact ionization of the molecular ion C 2 H 2 + have been carried out recently. These data differ significantly from earlier data, because cross-sections corresponding to all the possible dissociative ionization processes were determined. The new data in conjunction with the significant discrepancies between the earlier data and the results of various calculations, which disagreed among themselves by a factor of 3, motivated a renewed attempt to apply the semi-classical Deutsch-Mark (DM) formalism to the calculation of the absolute electron-impact ionization cross-section of this molecular ion. A quantum chemical molecular orbital population analysis for both the neutral molecule and the ion revealed that in the case of C 2 H 2 + the singly occupied molecular orbital (i.e. the 'missing' electron) is highly localized near the site of a C atom in the molecule. This information is explicitly incorporated in our formalism. The results obtained by taking the ionic character directly into account are in excellent agreement with the recent experimental data. (authors)

  5. Analytical formulas for dipole excitation cross sections of ions colliding with electrons

    International Nuclear Information System (INIS)

    Shevelko, V.P.

    1992-01-01

    Analytical formulas describing electron-impact induced dipole excitation of ions from threshold (E = ΔE) to high energies (E >> ΔE) are suggested. The formulas are based on the model dipole potential and are expressed in terms of McDonald functions. The results are compared with numerical calculations, other semiempirical formulas and experimental data

  6. Joint Experimental and Theoretical Study on Vibrational Excitation Cross Sections for Electron Collisions with Diacetylene

    Czech Academy of Sciences Publication Activity Database

    Čurík, Roman; Paidarová, Ivana; Allan, M.; Čársky, Petr

    2014-01-01

    Roč. 118, č. 41 (2014), s. 9734-9744 ISSN 1089-5639 R&D Projects: GA ČR GAP208/11/0452; GA MŠk LD14088 Institutional support: RVO:61388955 Keywords : diacetylenes * electron collision * theoretical study Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 2.693, year: 2014

  7. Active Cooling and Thermal Management of a Downhole Tool Electronics Section

    DEFF Research Database (Denmark)

    Soprani, Stefano; Engelbrecht, Kurt; Just Nørgaard, Anders

    2015-01-01

    combines active and passive cooling techniques, aiming at an efficient thermal management, preserving the tool compactness and avoiding the use of moving parts. Thermoelectric coolers were used to transfer the dissipated heat from the temperature-sensitive electronics to the external environment. Thermal...... contact resistances were minimized and thermally insulating foam protected the refrigerated microenvironment from the hot surroundings....

  8. Kilo-electron-volt neutron capture cross sections of the krypton isotopes

    International Nuclear Information System (INIS)

    Walter, G.; Leugers, B.; Kappeler; Bao, Z.Y.; Reffo, G.; Fabbri, F.

    1986-01-01

    The neutron capture cross sections of the stable krypton isotopes were determined in the energy interval from 4 to 250 keV using a C/sub 6/D/sub 6/-detector system in conjunction with the time-of-flight technique. The energy resolution of the measurement was 4% at 20 keV and 6% at 100 keV, and the experimental uncertainties were typically 6 to 10%. The measurements were complemented by statistical model calculations of all krypton isotopes in the mass range 78 < A < 86 to also obtain reliable cross sections for the unstable nuclei /sup 79,81,85/Kr. These calculations were based on local systematics for all relevant parameters, and the results were estimated to show uncertainties of 20 to 25%. Maxwellian average cross sections were calculated for kT=30 keV

  9. Multiple double cross-section transmission electron microscope sample preparation of specific sub-10 nm diameter Si nanowire devices.

    Science.gov (United States)

    Gignac, Lynne M; Mittal, Surbhi; Bangsaruntip, Sarunya; Cohen, Guy M; Sleight, Jeffrey W

    2011-12-01

    The ability to prepare multiple cross-section transmission electron microscope (XTEM) samples from one XTEM sample of specific sub-10 nm features was demonstrated. Sub-10 nm diameter Si nanowire (NW) devices were initially cross-sectioned using a dual-beam focused ion beam system in a direction running parallel to the device channel. From this XTEM sample, both low- and high-resolution transmission electron microscope (TEM) images were obtained from six separate, specific site Si NW devices. The XTEM sample was then re-sectioned in four separate locations in a direction perpendicular to the device channel: 90° from the original XTEM sample direction. Three of the four XTEM samples were successfully sectioned in the gate region of the device. From these three samples, low- and high-resolution TEM images of the Si NW were taken and measurements of the NW diameters were obtained. This technique demonstrated the ability to obtain high-resolution TEM images in directions 90° from one another of multiple, specific sub-10 nm features that were spaced 1.1 μm apart.

  10. The 4p6 autoionization cross section of Rb atoms excited by low-energy electron impact

    International Nuclear Information System (INIS)

    Borovik, A; Roman, V; Kupliauskienė, A

    2012-01-01

    The autoionization cross section of rubidium atoms was obtained by measuring the total normalized intensities of ejected-electron spectra arising from the decay of the 4p 5 n 1 l 1 n 2 l 2 autoionizing levels. The electron impact energy range from the 4p 6 excitation threshold at 15.31 up to 50 eV was investigated. The cross section reaches the maximum value of (2.9 ± 0.6) × 10 −16  cm 2 at 21.8 eV impact energy. The general behaviours of the cross section and the role of particular autoionizing configurations in its formation were considered on the basis of large-scale configuration interaction calculations of energies, cross sections, autoionization probabilities in 5snl(n ⩽ 7; l ⩽ 4) and 4d nl(n ⩽ 5; l ⩽ 2) configurations as well as the measured excitation functions for the lowest levels in 5s 2 and 4d5s configurations. The resonance behaviour of the cross section between 15.3 and 18.5 eV impact energy is caused exclusively by the negative-ion resonances present close to the excitation thresholds of the (5s 2 ) 2 P and (4d5s) 4 P autoionizing levels. At higher impact energies, the autoionization cross section is composed of contributions from the high-lying quartet and doublet levels in 4d5s, 5p and 5s5p, 5d, 6s, 6p configurations. From the comparison of the present data with available experimental and calculated ionization cross sections, the 5s + 4p 6 direct ionization cross section of rubidium atoms was determined with the maximum value of (7.2 ± 2.2) × 10 −16  cm 2 at 36 eV. It was also found that the 4p 6 excitation–autoionization is the dominant indirect ionization process contributing over 30% of the total single ionization of rubidium atoms by electron impact in the 15.3–50 eV energy range. (paper)

  11. Total cross section for Z0 production in electron proton collisions

    International Nuclear Information System (INIS)

    Salati, P.; Wallet, J.C.

    1982-01-01

    We present an exact calculation of the total cross section sigmasub(t)sub(o)sub(t) of the Z 0 production in e - +p→e - +Z 0 +X processes. We find a total cross section sigmasub(t)sub(o)sub(t)approx. equal to2.2x10 -36 cm 2 for an energy in the center of mass of 300 GeV and a Z 0 mass of 90 GeV, which is quite a measurable result in some e - proton collider experiments, like HERA or SPS/LEP. (orig.)

  12. Cross-sectional study of use of electronic media by secondary school students in Bangkok, Thailand.

    Science.gov (United States)

    Kiatrungrit, Komsan; Hongsanguansri, Sirichai

    2014-08-01

    There is increasing concern about the negative psychological effects of excessive use of various electronic media by adolescents but the monitoring of these behaviors in low- and middle-income countries has some methodological flaws. Assess the use of all types of electronic media among secondary school students in Bangkok, Thailand. A stratified random sample of students from four schools in Bangkok completed a modified version of a questionnaire used in a major study in the United States. Among the 768 participants, 443 (57.7%) were female and 325 (42.3%) were male; their mean (sd) age was 15.4 (1.5) years. Almost all respondents had easy access to multiple types of electronic media; 94% had mobile phones, 77% had a television in their bedroom, and 47% had internet access in their bedroom. Over the prior day 39% had watched television shows or movies for more than 3 hours, 28% spent more than 3 hours on social networking sites, 25% listened to music for more than 3 hours, and 18% played computer games for more than 3 hours. Overall, 27% reported using electronic devices for more than 12 hours in the previous day. Only 19% reported parental rules about the use of electronic devices in the home that were regularly enforced. Time engaged in the various activities was not related to parental education or, with the exception of time playing computer games, to students' grade point average. Younger students and male students spent less time than older students and female students using these devices to engage in interactive social activities (e.g., talking on the phone or social networking), while male students spent much more time than female students playing games on the devices. Adolescents spend a substantial part of every single day using different types of electronic devices. Longitudinal studies with precise time logs of device usage and descriptions of the type of content accessed are needed to determine the extent to which these activities have negative (or

  13. Measurements of the electron and muon inclusive cross-sections in ...

    Indian Academy of Sciences (India)

    and consists of three superconducting air-core toroids and a precise tracking system. The differential cross-section is determined with a bin-by-bin unfolding from the ... late π/K and hadronic shower remnants wrongly matched to an ID track ('fakes'). The background muon fraction is determined with a fit to the difference ...

  14. Electronic transport in graphene-based structures: An effective cross-section approach

    DEFF Research Database (Denmark)

    Uppstu, Andreas; Saloriutta, Karri; Harju, Ari

    2012-01-01

    We show that transport in low-dimensional carbon structures with finite concentrations of scatterers can be modeled by utilizing scaling theory and effective cross sections. Our results are based on large-scale numerical simulations of carbon nanotubes and graphene nanoribbons, using a tight...

  15. Estimating the thickness of ultra thin sections for electron microscopy by image statistics

    DEFF Research Database (Denmark)

    Sporring, Jon; Khanmohammadi, Mahdieh; Darkner, Sune

    2014-01-01

    We propose a method for estimating the thickness of ultra thin histological sections by image statistics alone. Our method works for images, that are the realisations of a stationary and isotropic stochastic process, and it relies on the existence of statistical image-measures that are strictly m...

  16. Metrics for Electronic-Nursing-Record-Based Narratives: Cross-sectional Analysis

    Science.gov (United States)

    Kim, Kidong; Jeong, Suyeon; Lee, Kyogu; Park, Hyeoun-Ae; Min, Yul Ha; Lee, Joo Yun; Kim, Yekyung; Yoo, Sooyoung; Doh, Gippeum

    2016-01-01

    Summary Objectives We aimed to determine the characteristics of quantitative metrics for nursing narratives documented in electronic nursing records and their association with hospital admission traits and diagnoses in a large data set not limited to specific patient events or hypotheses. Methods We collected 135,406,873 electronic, structured coded nursing narratives from 231,494 hospital admissions of patients discharged between 2008 and 2012 at a tertiary teaching institution that routinely uses an electronic health records system. The standardized number of nursing narratives (i.e., the total number of nursing narratives divided by the length of the hospital stay) was suggested to integrate the frequency and quantity of nursing documentation. Results The standardized number of nursing narratives was higher for patients aged 70 years (median = 30.2 narratives/day, interquartile range [IQR] = 24.0–39.4 narratives/day), long (8 days) hospital stays (median = 34.6 narratives/day, IQR = 27.2–43.5 narratives/day), and hospital deaths (median = 59.1 narratives/day, IQR = 47.0–74.8 narratives/day). The standardized number of narratives was higher in “pregnancy, childbirth, and puerperium” (median = 46.5, IQR = 39.0–54.7) and “diseases of the circulatory system” admissions (median = 35.7, IQR = 29.0–43.4). Conclusions Diverse hospital admissions can be consistently described with nursing-document-derived metrics for similar hospital admissions and diagnoses. Some areas of hospital admissions may have consistently increasing volumes of nursing documentation across years. Usability of electronic nursing document metrics for evaluating healthcare requires multiple aspects of hospital admissions to be considered. PMID:27901174

  17. Associations of attitudes towards electronic cigarettes with advertisement exposure and social determinants: a cross sectional study

    OpenAIRE

    Benjamin Reinhold; Rebecca Fischbein; Surya Sruthi Bhamidipalli; Jennifer Bryant; Deric R. Kenne

    2017-01-01

    Introduction The exposure of young adults to electronic cigarette (e-cigarette) advertisements has risen rapidly. E-cigarette ads have been shown to increase short term perceived acceptability of using e-cigarettes in places where traditional cigarettes are banned. We set out to investigate if advertising exposure was related to perceptions of harm, addictiveness, and acceptability of use of e-cigarettes in places where traditional cigarettes are banned. Material and Methods Using a...

  18. Total photoionization cross-sections of excited electronic states by the algebraic diagrammatic construction-Stieltjes-Lanczos method.

    Science.gov (United States)

    Ruberti, M; Yun, R; Gokhberg, K; Kopelke, S; Cederbaum, L S; Tarantelli, F; Averbukh, V

    2014-05-14

    Here, we extend the L2 ab initio method for molecular photoionization cross-sections introduced in Gokhberg et al. [J. Chem. Phys. 130, 064104 (2009)] and benchmarked in Ruberti et al. [J. Chem. Phys. 139, 144107 (2013)] to the calculation of total photoionization cross-sections of molecules in electronically excited states. The method is based on the ab initio description of molecular electronic states within the many-electron Green's function approach, known as algebraic diagrammatic construction (ADC), and on the application of Stieltjes-Chebyshev moment theory to Lanczos pseudospectra of the ADC electronic Hamiltonian. The intermediate state representation of the dipole operator in the ADC basis is used to compute the transition moments between the excited states of the molecule. We compare the results obtained using different levels of the many-body theory, i.e., ADC(1), ADC(2), and ADC(2)x for the first two excited states of CO, N2, and H2O both at the ground state and the excited state equilibrium or saddle point geometries. We find that the single excitation ADC(1) method is not adequate even at the qualitative level and that the inclusion of double electronic excitations for description of excited state photoionization is essential. Moreover, we show that the use of the extended ADC(2)x method leads to a substantial systematic difference from the strictly second-order ADC(2). Our calculations demonstrate that a theoretical modelling of photoionization of excited states requires an intrinsically double excitation theory with respect to the ground state and cannot be achieved by the standard single excitation methods with the ground state as a reference.

  19. Extraction of carbon 14-labeled compounds from plant tissue during processing for electron microscopy

    International Nuclear Information System (INIS)

    Coetzee, J.; van der Merwe, C.F.

    1989-01-01

    Loss of 14 C-labeled compounds from bean leaf tissue was monitored during all the stages of routine specimen preparation. No significant differences in extraction were associated with the use of acetone, ethanol, or dioxane as dehydration fluids. Fixation at low temperature increased the loss of label. Prolonged fixation in glutaraldehyde increased the loss, but fixation in osmium solutions for periods as long as 4 hr had no influence on extraction. Buffer rinses and dehydration fluids caused appreciable amounts of label to be extracted. The use of propylene oxide as transition fluid resulted in low extraction. Some embedding media caused the loss of small amounts of labeled compounds, but one of the media tested (LR-white) extracted significant amounts of label

  20. A texture based pattern recognition approach to distinguish melanoma from non-melanoma cells in histopathological tissue microarray sections.

    Directory of Open Access Journals (Sweden)

    Elton Rexhepaj

    Full Text Available AIMS: Immunohistochemistry is a routine practice in clinical cancer diagnostics and also an established technology for tissue-based research regarding biomarker discovery efforts. Tedious manual assessment of immunohistochemically stained tissue needs to be fully automated to take full advantage of the potential for high throughput analyses enabled by tissue microarrays and digital pathology. Such automated tools also need to be reproducible for different experimental conditions and biomarker targets. In this study we present a novel supervised melanoma specific pattern recognition approach that is fully automated and quantitative. METHODS AND RESULTS: Melanoma samples were immunostained for the melanocyte specific target, Melan-A. Images representing immunostained melanoma tissue were then digitally processed to segment regions of interest, highlighting Melan-A positive and negative areas. Color deconvolution was applied to each region of interest to separate the channel containing the immunohistochemistry signal from the hematoxylin counterstaining channel. A support vector machine melanoma classification model was learned from a discovery melanoma patient cohort (n = 264 and subsequently validated on an independent cohort of melanoma patient tissue sample images (n = 157. CONCLUSION: Here we propose a novel method that takes advantage of utilizing an immuhistochemical marker highlighting melanocytes to fully automate the learning of a general melanoma cell classification model. The presented method can be applied on any protein of interest and thus provides a tool for quantification of immunohistochemistry-based protein expression in melanoma.

  1. Electronic cigarette use among adolescents: a cross-sectional study in Hong Kong

    OpenAIRE

    Nan Jiang; Man Ping Wang; Sai Yin Ho; Lok Tung Leung; Tai Hing Lam

    2016-01-01

    Abstract Background Little is known about electronic cigarette (e-cigarette) use among Chinese adolescents. We examined the prevalence of current (past 30-day) e-cigarette use and its associated factors in a large sample of adolescents in Hong Kong. Methods We analyzed data of the School-based Survey on Smoking among Students 2012/13 from a representative sample of 45,857 secondary school students (mean age: 14.8 ± 1.9). We conducted chi-square tests and t-test to compare current e-cigarette ...

  2. A practical approach to temperature effects in dissociative electron attachment cross sections using local complex potential theory

    International Nuclear Information System (INIS)

    Sugioka, Yuji; Takayanagi, Toshiyuki

    2012-01-01

    Highlights: ► Dissociative electron attachment cross sections for polyatomic molecules are calculated by a simple theoretical approach. ► Temperature effects can be reasonably reproduced with the present model. ► All the degrees-of-freedom are taken into account in the present dynamics approach. -- Abstract: We propose a practical computational scheme to obtain temperature dependence of dissociative electron attachment cross sections to polyatomic molecules within a local complex potential theory formalism. First we perform quantum path-integral molecular dynamics simulations on the potential energy surface for the neutral molecule in order to sample initial nuclear configurations as well as momenta. Classical trajectories are subsequently integrated on the potential energy surface for the anionic state and survival probabilities are simultaneously calculated along the obtained trajectories. We have applied this simple scheme to dissociative electron attachment processes to H 2 O and CF 3 Cl, for which several previous studies are available from both the experimental and theoretical sides.

  3. Measurements of Coulomb Cross Section for Production of Direct Electron-pairs by High Energy Ions at the CERN SPS

    CERN Multimedia

    2002-01-01

    QED predicts copious direct electron pair production by ultrarelativistic heavy nuclei in a high Z medium such as nuclear emulsion. First order QED calculations (combined screening and non-screening) for this process show that 1000@+32 electron pairs above 100~keV energy) should be emitted for a total |1|6O track length of 10.9~m in nuclear emulsion at 200~GeV/AMU. Emulsion exposures with oxygen (and other nuclei if available) at 60 and 200~GeV/AMU will be used to calibrate the energy dependent cross section @s~@j~(1n~E)|2|-|3, whose exponent depends on atomic screening. The oxygen tracks in the developed emulsions will be scanned with a microscope, and the number of direct electron pairs will be counted for individual tracks. The exposed stacks will contain sufficient emulsion (and CR39 plastic to check for possible interactions) that adequate path length will be available for exposures to @$>$~10|4~ions at each energy and ion species. \\\\ \\\\ If the absolute value of this cross section is confirmed as large a...

  4. A practical approach to temperature effects in dissociative electron attachment cross sections using local complex potential theory

    Energy Technology Data Exchange (ETDEWEB)

    Sugioka, Yuji [Department of Chemistry, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570 (Japan); Takayanagi, Toshiyuki, E-mail: tako@mail.saitama-u.ac.jp [Department of Chemistry, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570 (Japan)

    2012-09-11

    Highlights: Black-Right-Pointing-Pointer Dissociative electron attachment cross sections for polyatomic molecules are calculated by a simple theoretical approach. Black-Right-Pointing-Pointer Temperature effects can be reasonably reproduced with the present model. Black-Right-Pointing-Pointer All the degrees-of-freedom are taken into account in the present dynamics approach. -- Abstract: We propose a practical computational scheme to obtain temperature dependence of dissociative electron attachment cross sections to polyatomic molecules within a local complex potential theory formalism. First we perform quantum path-integral molecular dynamics simulations on the potential energy surface for the neutral molecule in order to sample initial nuclear configurations as well as momenta. Classical trajectories are subsequently integrated on the potential energy surface for the anionic state and survival probabilities are simultaneously calculated along the obtained trajectories. We have applied this simple scheme to dissociative electron attachment processes to H{sub 2}O and CF{sub 3}Cl, for which several previous studies are available from both the experimental and theoretical sides.

  5. Cross sections of electron excitation out of metastable helium levels with a fast metastable target product produced via charge exchange

    International Nuclear Information System (INIS)

    Lagus, M.E.; Boffard, J.B.; Anderson, L.W.; Lin, C.C.

    1996-01-01

    Absolute direct cross sections for electron excitation out of the 2 3 S level and into the 3 3 D, 4 3 D, and 3 3 S levels of the helium atom from threshold to 500 eV and into the 3 3 P level over a more limited energy range have been measured using a fast metastable atomic beam target. We produce the metastable atoms via near-resonant charge exchange between a 1.6-keV He + ion beam and Cs vapor. Because this reaction is highly nonresonant with the ground state of helium, the charge-transfer process yields a primarily metastable beam. We use a thermal detector which we calibrate with ions to measure absolutely the neutral beam flux. The atomic beam is crossed by an electron beam, and we collect the resulting fluorescence at right angles to both the electron and atomic beams. We obtain the cross sections for excitation out of the 2 3 S level into the various excited levels by monitoring the emission out of the excited level of interest. copyright 1996 The American Physical Society

  6. Correction of the near threshold behavior of electron collisional excitation cross-sections in the plane-wave Born approximation

    Science.gov (United States)

    Kilcrease, D. P.; Brookes, S.

    2013-12-01

    The modeling of NLTE plasmas requires the solution of population rate equations to determine the populations of the various atomic levels relevant to a particular problem. The equations require many cross sections for excitation, de-excitation, ionization and recombination. A simple and computational fast way to calculate electron collisional excitation cross-sections for ions is by using the plane-wave Born approximation. This is essentially a high-energy approximation and the cross section suffers from the unphysical problem of going to zero near threshold. Various remedies for this problem have been employed with varying degrees of success. We present a correction procedure for the Born cross-sections that employs the Elwert-Sommerfeld factor to correct for the use of plane waves instead of Coulomb waves in an attempt to produce a cross-section similar to that from using the more time consuming Coulomb Born approximation. We compare this new approximation with other, often employed correction procedures. We also look at some further modifications to our Born Elwert procedure and its combination with Y.K. Kim's correction of the Coulomb Born approximation for singly charged ions that more accurately approximate convergent close coupling calculations.

  7. Brain tissues atrophy is not always the best structural biomarker of physiological aging: A multimodal cross-sectional study.

    Science.gov (United States)

    Cherubini, Andrea; Caligiuri, Maria Eugenia; Péran, Patrice; Sabatini, Umberto; Cosentino, Carlo; Amato, Francesco

    2015-01-01

    This study presents a voxel-based multiple regression analysis of different magnetic resonance image modalities, including anatomical T1-weighted, T2* relaxometry, and diffusion tensor imaging. Quantitative parameters sensitive to complementary brain tissue alterations, including morphometric atrophy, mineralization, microstructural damage, and anisotropy loss, were compared in a linear physiological aging model in 140 healthy subjects (range 20-74 years). The performance of different predictors and the identification of the best biomarker of age-induced structural variation were compared without a priori anatomical knowledge. The best quantitative predictors in several brain regions were iron deposition and microstructural damage, rather than macroscopic tissue atrophy. Age variations were best resolved with a combination of markers, suggesting that multiple predictors better capture age-induced tissue alterations. These findings highlight the importance of a combined evaluation of multimodal biomarkers for the study of aging and point to a number of novel applications for the method described.

  8. Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue

    DEFF Research Database (Denmark)

    Kirkeby, S

    1981-01-01

    In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline...... phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained....

  9. Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.

    Science.gov (United States)

    Delpiano, J; Pizarro, L; Peddie, C J; Jones, M L; Griffin, L D; Collinson, L M

    2018-04-26

    Integrated array tomography combines fluorescence and electron imaging of ultrathin sections in one microscope, and enables accurate high-resolution correlation of fluorescent proteins to cell organelles and membranes. Large numbers of serial sections can be imaged sequentially to produce aligned volumes from both imaging modalities, thus producing enormous amounts of data that must be handled and processed using novel techniques. Here, we present a scheme for automated detection of fluorescent cells within thin resin sections, which could then be used to drive automated electron image acquisition from target regions via 'smart tracking'. The aim of this work is to aid in optimization of the data acquisition process through automation, freeing the operator to work on other tasks and speeding up the process, while reducing data rates by only acquiring images from regions of interest. This new method is shown to be robust against noise and able to deal with regions of low fluorescence. © 2018 The Authors. Journal of Microscopy published by JohnWiley & Sons Ltd on behalf of Royal Microscopical Society.

  10. Effect of high energy electrons on the skin and on the underlying tissues of the rabbit. A clinical and histological study

    International Nuclear Information System (INIS)

    Legeay, G.; Vialettes, H.; Adnet, J.J.; Court, L.; Masse, R.

    1967-01-01

    The authors consider in this report the effects of high-energy electrons on rabbit teguments and on the underlying tissues after a single high dose irradiation. After briefly considering the mechanism of interaction between the electrons and matter as a function of their energy, the authors describe the dosimetry carried out, as a function of the irradiation device. The animal received surface doses of 5700 to 22100 rads in the thigh; the electron energy varied from 21 to 30 MeV. A clinical study was carried out over a period of nine months with a view to following the evolution of the damage and the functional degradation of the underlying tissues. A histological study of the induced damage was made after a second irradiation using 30 MeV electrons to produce doses of 16400 rads. Interesting observations were made concerning the damage caused to muscular and nerve tissues. (authors) [fr

  11. Differential cross sections for rovibrational (v'=0→1,2,3,4) excitation of the electronic ground state of O2 by electron impact

    International Nuclear Information System (INIS)

    Brunger, M.J.; Middleton, A.G.; Teubner, P.J.

    1998-01-01

    We have measured absolute differential cross sections (DCSs) for rovibrational excitation (0→1,2,3,4) of the χ 3 Σ g - electronic ground state of O 2 . The scattered electron angular range was 10 degree endash 90 degree, while the cross sections were measured at ten specific energies in the 5 endash 20 eV incident beam energy range. This energy regime encompasses the region where the 4 Σ u - and 2 Σ u - resonances of O 2 - are known to significantly enhance the vibrational excitation process. The present data are found to be in generally good agreement with the more limited recent measurements of Allan [J. Phys. B 28, 5163 (1995)] and the earlier results of Wong et al. [Phys. Rev. Lett. 31, 969 (1973)]. Agreement with the data of Shyn and Sweeney [Phys. Rev. A 48, 1214 (1993)], however, was found to be only fair. Comparison of the present DCS with an available Born-closure Schwinger variational method calculation result is also made. copyright 1998 The American Physical Society

  12. 3D imaging of cells and tissues by focused ion beam/scanning electron microscopy (FIB/SEM).

    Science.gov (United States)

    Drobne, Damjana

    2013-01-01

    Integration of a scanning electron microscope (SEM) and focused ion beam (FIB) technology into a single FIB/SEM system permits use of the FIB as a nano-scalpel to reveal site-specific subsurface microstructures which can be examined in great detail by SEM. The FIB/SEM technology is widely used in the semiconductor industry and material sciences, and recently its use in the life sciences has been initiated. Samples for FIB/SEM investigation can be either embedded in a plastic matrix, the traditional means of preparation of transmission electron microscopy (TEM) specimens, or simply dried as in samples prepared for SEM imaging. Currently, FIB/SEM is used in the life sciences for (a) preparation by the lift-out technique of lamella for TEM analysis, (b) tomography of samples embedded in a matrix, and (c) in situ site-specific FIB milling and SEM imaging using a wide range of magnifications. Site-specific milling and imaging has attracted wide interest as a technique in structural research of single eukaryotic and prokaryotic cells, small animals, and different animal tissue, but it still remains to be explored more thoroughly. In the past, preparation of samples for site-specific milling and imaging by FIB/SEM has typically adopted the embedding techniques used for TEM samples, and which have been very well described in the literature. Sample preparation protocols for the use of dried samples in FIB/SEM have been less well investigated. The aim of this chapter is to encourage application of FIB/SEM on dried biological samples. A detailed description of conventional dried sample preparation and FIB/SEM investigation of dried biological samples is presented. The important steps are described and illustrated, and direct comparison between embedded and dried samples of same tissues is provided. The ability to discover links between gross morphology of the tissue or organ, surface characteristics of any selected region, and intracellular structural details on the nanometer

  13. Critically assessed electron-impact excitation cross sections for He(11S)

    International Nuclear Information System (INIS)

    Heer, F.J. de

    1998-11-01

    In this paper we reconsider the previous assessment of collision strengths, now mostly presented as cross sections, for excitation of He(1 1 S) to He(n 1,3 L) states (n=2-4, L=0-3). Due to the appearance of additional theoretical results the assessment can now be often given within smaller error limits than before for the singlet levels, as well as for the lower triplet levels (n=2). For the higher excited triplet states more studies are desirable to reduce the possible errors. (author)

  14. A novel, flat, electronically-steered phased array transducer for tissue ablation: preliminary results

    International Nuclear Information System (INIS)

    Ellens, Nicholas P K; Lucht, Benjamin B C; Gunaseelan, Samuel T; Hudson, John M; Hynynen, Kullervo H

    2015-01-01

    Flat, λ/2-spaced phased arrays for therapeutic ultrasound were examined in silico and in vitro. All arrays were made by combining modules made of 64 square elements with 1.5 mm inter-element spacing along both major axes. The arrays were designed to accommodate integrated, co-aligned diagnostic transducers for targeting and monitoring. Six arrays of 1024 elements (16 modules) and four arrays of 6144 elements (96 modules) were modelled and compared according to metrics such as peak pressure amplitude, focal size, ability to be electronically-steered far off-axis and grating lobe amplitude. Two 1024 element prototypes were built and measured in vitro, producing over 100 W of acoustic power. In both cases, the simulation model of the pressure amplitude field was in good agreement with values measured by hydrophone. Using one of the arrays, it was shown that the peak pressure amplitude dropped by only 24% and 25% of the on-axis peak pressure amplitude when steered to the edge of the array (40 mm) at depths of 30 mm and 50 mm. For the 6144 element arrays studied in in silico only, similarly high steerability was found: even when steered 100 mm off-axis, the pressure amplitude decrease at the focus was less than 20%, while the maximum pressure grating lobe was only 20%. Thermal simulations indicate that the modules produce more than enough acoustic power to perform rapid ablations at physiologically relevant depths and steering angles. Arrays such as proposed and tested in this study have enormous potential: their high electronic steerability suggests that they will be able to perform ablations of large volumes without the need for any mechanical translation. (paper)

  15. Backscattered electron imaging: The role in calcified tissue and implant analysis

    International Nuclear Information System (INIS)

    Bloebaum, R.D.; Bachus, K.N.; Boyce, T.M.

    1990-01-01

    The working distance and tilt studies helped to clarify the influences of specimen variability when the BSE mode is used in calcified tissue research. This work has shown that the BSEPs of cortical bone may be accurately maintained within 2 percent error over a 10 degree range of tilt, or 300 microns working distance variation. If future bone and implant investigators wish to conduct accurate, quantitative mineral microanalysis in bone, then standard grinding and polishing techniques should be adequate if calibration procedures are developed. The BSEP characteristics of the pure metals make them suitable to be used for calibrating the BSE signal. BSE analysis, with correlated biomechanical studies, will lead us to a better understanding of the relationships between structure, function, and mineral content in bone. On-line BSEP analysis techniques will expand our understanding of the mineralization events in bone which are associated with aging, weightlessness, pharmaceutical therapies, and the presence of biomaterials. The future of the BSE imaging technology and the contributions to be made in understanding the histometry, biomechanics and mineral content of bone as well as bone's response to implant materials has just begun to unfold. 74 references

  16. Development and Characterization of Organic Electronic Scaffolds for Bone Tissue Engineering.

    Science.gov (United States)

    Iandolo, Donata; Ravichandran, Akhilandeshwari; Liu, Xianjie; Wen, Feng; Chan, Jerry K Y; Berggren, Magnus; Teoh, Swee-Hin; Simon, Daniel T

    2016-06-01

    Bones have been shown to exhibit piezoelectric properties, generating electrical potential upon mechanical deformation and responding to electrical stimulation with the generation of mechanical stress. Thus, the effects of electrical stimulation on bone tissue engineering have been extensively studied. However, in bone regeneration applications, only few studies have focused on the use of electroactive 3D biodegradable scaffolds at the interphase with stem cells. Here a method is described to combine the bone regeneration capabilities of 3D-printed macroporous medical grade polycaprolactone (PCL) scaffolds with the electrical and electrochemical capabilities of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT). PCL scaffolds have been highly effective in vivo as bone regeneration grafts, and PEDOT is a leading material in the field of organic bioelectronics, due to its stability, conformability, and biocompatibility. A protocol is reported for scaffolds functionalization with PEDOT, using vapor-phase polymerization, resulting in a conformal conducting layer. Scaffolds' porosity and mechanical stability, important for in vivo bone regeneration applications, are retained. Human fetal mesenchymal stem cells proliferation is assessed on the functionalized scaffolds, showing the cytocompatibility of the polymeric coating. Altogether, these results show the feasibility of the proposed approach to obtain electroactive scaffolds for electrical stimulation of stem cells for regenerative medicine. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Measurement of beauty and charm cross sections in photoproduction using decays into electrons with ZEUS at HERA

    Energy Technology Data Exchange (ETDEWEB)

    Juengst, Markus

    2010-02-15

    Photoproduction of heavy quarks in events with two jets and an electron associated with one of the jets has been studied with the ZEUS detector using the data recorded in the years 1996-2000 and 2006-2007, corresponding to an integrated luminosity of L=120 pb{sup -1} and L=190 pb{sup -1}, respectively. The fractions of events containing b quarks, and also of events containing c quarks, were extracted from a likelihood fit using variables sensitive to electron identification as well as to semileptonic decays. For the second data-set the list of variables sensitive to the flavour could be extended using the additional information from the microvertexdetector. Total and differential cross sections for beauty and charm production were measured for both data-sets and compared with next-to-leading-order QCD calculations and Monte Carlo models. (orig.)

  18. Absolute vibrational excitation cross sections for 1-18 eV electron scattering from condensed dimethyl phosphate (DMP)

    Science.gov (United States)

    Lemelin, V.; Bass, A. D.; Wagner, J. R.; Sanche, L.

    2017-12-01

    Absolute cross sections (CSs) for vibrational excitation by 1-18 eV electrons incident on condensed dimethyl phosphate (DMP) were measured with a high-resolution electron energy loss (EEL) spectrometer. Absolute CSs were extracted from EEL spectra of DMP condensed on multilayer film of Ar held at about 20 K under ultra-high vacuum (˜1 × 10-11 Torr). Structures observed in the energy dependence of the CSs around 2, 4, 7, and 12 eV were compared with previous results of gas- and solid-phase experiments and with theoretical studies on dimethyl phosphate and related molecules. These structures were attributed to the formation of shape resonances.

  19. Measurement of beauty and charm cross sections in photoproduction using decays into electrons with ZEUS at HERA

    International Nuclear Information System (INIS)

    Juengst, Markus

    2010-02-01

    Photoproduction of heavy quarks in events with two jets and an electron associated with one of the jets has been studied with the ZEUS detector using the data recorded in the years 1996-2000 and 2006-2007, corresponding to an integrated luminosity of L=120 pb -1 and L=190 pb -1 , respectively. The fractions of events containing b quarks, and also of events containing c quarks, were extracted from a likelihood fit using variables sensitive to electron identification as well as to semileptonic decays. For the second data-set the list of variables sensitive to the flavour could be extended using the additional information from the microvertexdetector. Total and differential cross sections for beauty and charm production were measured for both data-sets and compared with next-to-leading-order QCD calculations and Monte Carlo models. (orig.)

  20. Cross sections for electron-impact excitation of the H2 molecule using the MOB-SCI strategy

    International Nuclear Information System (INIS)

    Costa, Romarly F da; Paixao, Fernando J da; Lima, Marco A P

    2005-01-01

    In this paper, we report integral and differential cross sections for the electronic excitation of H 2 molecules by electron-impact. Our scattering amplitudes were calculated using the Schwinger multichannel method within the minimal orbital basis for single configuration interactions (MOB-SCI) level of approximation. Through the use of the present strategy we have investigated the coupling effects among ground state and first singlet and triplet states of the same spatial symmetry. The five-state (nine for degenerated states) close-coupling calculations joined the advantages of a well-described set of physical states of interest with a minimum associated pseudo-state space. The results obtained by means of the MOB-SCI technique show a significant improvement towards experimental data in comparison with previous two-channel close-coupling calculations

  1. High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

    Science.gov (United States)

    Nguyen, Peter L.; Davidson, Bennett; Akkina, Sanjeev; Guzman, Grace; Setty, Suman; Kajdacsy-Balla, Andre; Walsh, Michael J.

    2015-01-01

    High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and composition of biomolecules (e.g., lipids, DNA, glycogen, protein, collagen). In an FT-IR image, every pixel within the image comprises an entire Infrared (IR) spectrum that can give information on the biochemical status of the cells that can then be exploited for cell-type or disease-type classification. In this paper, we show: how to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualize FT-IR images. We then present some applications of FT-IR for pathology using the liver and kidney as examples. FT-IR imaging holds exciting applications in providing a novel route to obtain biochemical information from cells and tissue in an entirely label-free non-perturbing route towards giving new insight into biomolecular changes as part of disease processes. Additionally, this biochemical information can potentially allow for objective and automated analysis of certain aspects of disease diagnosis. PMID:25650759

  2. Registration for deceased organ and tissue donation among Ontario immigrants: a population-based cross-sectional study.

    Science.gov (United States)

    Li, Alvin Ho-Ting; Lam, Ngan N; Dhanani, Sonny; Weir, Matthew; Prakash, Versha; Kim, Joseph; Knoll, Greg; Garg, Amit X

    2016-01-01

    Canada has low rates of deceased organ and tissue donation. Immigrants to Canada may differ in their registered support for deceased organ donation based on their country of origin. We used linked administrative databases in Ontario (about 11 million residents aged ≥ 16 yr) to study the proportion of immigrants and long-term residents registered for deceased organ and tissue donation as of October 2013. We used modified Poisson regression to identify and quantify predictors of donor registration. Compared with long-term residents ( n = 9 244 570), immigrants ( n = 1 947 646) were much less likely to register for deceased organ and tissue donation (11.9% v. 26.5%). Immigrants from the United States, Australia and New Zealand had the highest registration rate (40.0%), whereas immigrants with the lowest registration rates were from Eastern Europe and Central Asia (9.4%), East Asia and Pacific (8.4%) and sub-Saharan Africa (7.9%). The largest numbers of unregistered immigrants were from India ( n = 202 548), China ( n = 186 678) and the Philippines ( n = 125 686). Characteristics among the immigrant population associated with a higher likelihood of registration included economic immigrant status, living in a rural area (population speak English and French, and more years residing in Canada. Immigrants in Ontario were less likely to register for deceased organ and tissue donation than long-term residents. There is a need to better understand reasons for lower registration rates among Canadian immigrants and to create culture-sensitive materials to build support for deceased organ and tissue donation.

  3. Precise measurement of the left-right cross section asymmetry in Z boson production by electron-positron collisions

    International Nuclear Information System (INIS)

    Frey, R.E.

    1994-03-01

    A precise measurement of the left-right cross section asymmetry (A LR ) for Z boson production by e + e - collisions has been attained at the Slac Linear Collider with the SLD detector. We describe this measurement for the 1993 data run, emphasizing the significant improvements in polarized beam operation which took place for this run, where the luminosity-weighted electron beam polarization averaged 62.6 ± 1.2 %. Preliminary 1993 results for A LR are presented. When combined with the (less precise) 1992 result, the preliminary result for the effective weak mixing angle is sin 2 θ W eff = 0.2290 ± 0.0010

  4. Measurement of the High-Mass Drell-Yan Cross Section and Limits on Quark-Electron Compositeness Scales

    International Nuclear Information System (INIS)

    Grinstein, S.; Mostafa, M.; Piegaia, R.; Alves, G.A.; Carvalho, W.; Maciel, A.K.; Motta, H. da; Oliveira, E.; Santoro, A.; Lima, J.G.; Oguri, V.; Gomez, B.; Hoeneisen, B.; Mooney, P.; Negret, J.P.; Ducros, Y.; Beri, S.B.; Bhatnagar, V.; Kohli, J.M.; Singh, J.B.; Shivpuri, R.K.; Acharya, B.S.; Banerjee, S.; Dugad, S.R.; Gupta, A.; Krishnaswamy, M.R.; Mondal, N.K.; Narasimham, V.S.; Parua, N.; Shankar, H.C.; Park, Y.M.; Choi, S.; Kim, S.K.; Castilla-Valdez, H.; Gonzalez Solis, J.L.; Hernandez-Montoya, R.; Magana-Mendoza, L.; Sanchez-Hernandez, A.; Pawlik, B.; Gavrilov, V.; Gershtein, Y.; Kuleshov, S.; Belyaev, A.; Dudko, L.V.; Ermolov, P.; Karmanov, D.; Leflat, A.; Manankov, V.; Merkin, M.; Shabalina, E.; Abramov, V.; Babintsev, V.V.; Bezzubov, V.A.; Bojko, N.I.; Burtovoi, V.S.; Chekulaev, S.V.; Denisov, S.P.; Dyshkant, A.; Eroshin, O.V.; Evdokimov, V.N.; Galyaev, A.N.; Goncharov, P.I.; Gurzhiev, S.N.; Kostritskiy, A.V.; Kozelov, A.V.; Kozlovsky, E.A.; Mayorov, A.A.; Babukhadia, L.; Davis, K.; Fein, D.; Forden, G.E.; Guida, J.A.; Johns, K.; Nang, F.; Narayanan, A.; Rutherfoord, J.; Shupe, M.; Aihara, H.; Barberis, E.; Clark, A.R.

    1999-01-01

    We present a measurement of the Drell-Yan cross section at high dielectron invariant mass using 120 pb -1 of data collected in p bar p collisions at √ (s) =1.8 TeV by the D0 Collaboration during 1992 - 1996. No deviation from standard model expectations is observed. We use the data to set limits on the quark-electron compositeness scale. The 95% confidence level lower limits on the compositeness scale vary between 3.3 and 6.1thinspthinspTeV depending on the assumed form of the effective contact interaction. copyright 1999 The American Physical Society

  5. Cross sections for one-electron capture by highly stripped ions of Be, B and C from H2 and Ar below 10 keV

    International Nuclear Information System (INIS)

    Takagi, S.; Ohtani, S.; Kadota, K.; Fujita, J.

    1982-03-01

    Cross sections for one-electron capture by highly stripped ions of Be, B and C from H 2 and Ar are measured at low energies below 10 keV. The cross sections are nearly independent of the collision energy investigated. The distinct oscillation with incident ionic charge g in the cross sections are observed. (author)

  6. Electron-capture cross sections for low-energy highly charged neon and argon ions from molecular and atomic hydrogen

    International Nuclear Information System (INIS)

    Can, C.; Gray, T.J.; Varghese, S.L.; Hall, J.M.; Tunnell, L.N.

    1985-01-01

    Electron-capture cross sections for low-velocity (10 6 --10 7 cm/s) highly charged Ne/sup q/+ (2< or =q< or =7) and Ar/sup q/+ (2< or =q< or =10)= projectiles incident on molecular- and atomic-hydrogen targets have been measured. A recoil-ion source that used the collisions of fast heavy ions (1 MeV/amu) with target gas atoms was utilized to produce slow highly charged ions. Atomic hydrogen was produced by dissociating hydrogen molecules in a high-temperature oven. Measurements and analysis of the data for molecular- and atomic-hydrogen targets are discussed in detail. The measured absolute cross sections are compared with published data and predictions of theoretical models

  7. Averaged electron collision cross sections for thermal mixtures of β-alanine conformers in the gas phase

    Science.gov (United States)

    Fujimoto, Milton M.; de Lima, Erik V. R.; Tennyson, Jonathan

    2017-10-01

    A theoretical study of elastic electron scattering by gas-phase amino acid molecule β-alanine (NH2-CH2-CH2-COOH) is presented. R-matrix calculations are performed for each of the ten lowest-lying, thermally-accessible conformers of β-alanine. Eigenphase sums, resonance features, differential and integral cross sections are computed for each conformer. The positions of the low-energy shape resonance associated with the unoccupied {π }* orbital of the -COOH group are found to vary from 2.5 to 3.3 eV and the resonance widths from 0.2 to 0.5 eV depending on the conformation. The temperature-dependent population ratios are derived, based on temperature-corrected Gibbs free energies. Averaged cross sections for thermal mixtures of the 10 conformers are presented. A comparison with previous results for the α-alanine isomer is also presented.

  8. Effect of different electron elastic-scattering cross sections on inelastic mean free paths obtained from elastic-backscattering experiments

    International Nuclear Information System (INIS)

    Jablonskiz, A.; Salvatz, F.; Powellz, C.J.

    2004-01-01

    Inelastic mean free paths (IMFPs) of electrons with energies between 100 eV and 5,000 eV have been frequently obtained from measurements of elastic-backscattering probabilities for different specimen materials. A calculation of these probabilities is also required to determine IMFPs. We report calculations of elastic-backscattering probabilities for gold at energies of 100 eV and 500 eV with differential elastic-scattering cross sections obtained from the Thomas-Fermi-Dirac potential and the more reliable Dirac-Hartree-Fock potential. For two representative experimental configurations, the average deviation between IMFPs obtained with cross sections from the two potentials was 11.4 %. (author)

  9. Analyses of the eustachian tube and its surrounding tissues with cross sectional images by high-resolution computed tomography (HR-CT)

    International Nuclear Information System (INIS)

    Yoshida, Haruo; Kobayashi, Toshimitsu; Takasaki, Kenji; Kanda, Yukihiko; Nakao, Yoshiaki; Morikawa, Minoru; Ishimaru, Hideki; Hayashi, Kuniaki

    2000-01-01

    We attempted to image the eustachian tube (ET) and its surrounding tissues by high-resolution computed tomography (HR-CT). Twenty-two normal subjects (44 ears) without middle ear problems were studied, and a patient with severe patulous ET was also studied as an abnormal case. In our device of multiplanar reconstruction technique, we were able to obtain the clear reconstructed images of the ET lumen as well as of its surrounding tissues (bone, ET cartilage, tensor veli palatini muscle, levator veli palatini muscle, Ostmann's fat tissue, tensor tympani muscle, internal carotid artery) at any desired portion, either parallel or perpendicular to the long axis of the ET. However, the exact borders between the ET cartilage and the muscles, Ostmann's fat tissue and the tubal gland were not clearly identified. In the severe case of patulous ET, the ET lumen was widely opened at each cross-sectional image from the pharyngeal orifice to the tympanic orifice, in contrast with its being closed at the cartilaginous portion in the normal cases. In addition, the fat tissue and glands around the ET lumen were not clearly identified in this case. We suggest that this method will lead to better understanding of the ET-related diseases such as patulous ET. (author)

  10. Analyses of the eustachian tube and its surrounding tissues with cross sectional images by high-resolution computed tomography (HR-CT)

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Haruo; Kobayashi, Toshimitsu; Takasaki, Kenji; Kanda, Yukihiko; Nakao, Yoshiaki; Morikawa, Minoru; Ishimaru, Hideki; Hayashi, Kuniaki [Nagasaki Univ. (Japan). School of Medicine

    2000-07-01

    We attempted to image the eustachian tube (ET) and its surrounding tissues by high-resolution computed tomography (HR-CT). Twenty-two normal subjects (44 ears) without middle ear problems were studied, and a patient with severe patulous ET was also studied as an abnormal case. In our device of multiplanar reconstruction technique, we were able to obtain the clear reconstructed images of the ET lumen as well as of its surrounding tissues (bone, ET cartilage, tensor veli palatini muscle, levator veli palatini muscle, Ostmann's fat tissue, tensor tympani muscle, internal carotid artery) at any desired portion, either parallel or perpendicular to the long axis of the ET. However, the exact borders between the ET cartilage and the muscles, Ostmann's fat tissue and the tubal gland were not clearly identified. In the severe case of patulous ET, the ET lumen was widely opened at each cross-sectional image from the pharyngeal orifice to the tympanic orifice, in contrast with its being closed at the cartilaginous portion in the normal cases. In addition, the fat tissue and glands around the ET lumen were not clearly identified in this case. We suggest that this method will lead to better understanding of the ET-related diseases such as patulous ET. (author)

  11. Detection of EGFR and COX-2 Expression by Immunohistochemical Method on a Tissue Microarray Section in Lung Cancer and Biological Significance

    Directory of Open Access Journals (Sweden)

    Xinyun WANG

    2010-02-01

    Full Text Available Background and objective Epidermal growth factor receptor (EGFR and cyclooxygenase-2 (COX-2, which can regulate growth, invasion and metastasis of tumor through relevant signaling pathway, have been detected in a variety of solid tumors. The aim of this study is to investigate the biological significance of EGFR and COX-2 expression in lung cancer and the relationship between them. Methods The expression of EGFR and COX-2 was detected in 89 primary lung cancer tissues, 12 premaliganant lesions, 12 lymph node metastases, and 10 normal lung tissues as the control by immunohistochemical method on a tissue microarray section. Results EGFR protein was detectable in 59.6%, 41.7%, and 66.7% of primary lung cancer tissues, premalignant lesions and lymph node metastases, respectively; COX-2 protein was detectable in 52.8%, 41.7%, and 66.7% of primary lung cancer tissues, premalignant lesions and lymph node metastases, respectively, which were significantly higher than those of the control (P 0.05. COX-2 expression was related to gross type (P < 0.05. A highly positive correlation was observed between EGFR and COX-2 expression (P < 0.01. Conclusion Overexpression of EGFR and COX-2 may play an important role in the tumorgenesis, progression and malignancy of lung cancer. Detection of EGFR and COX-2 expression might be helpful to diagnosis and prognosis of lung cancer.

  12. Effects of gamma radiation on hard dental tissues of albino rats using scanning electron microscope - Part 1

    Science.gov (United States)

    El-Faramawy, Nabil; Ameen, Reham; El-Haddad, Khaled; Maghraby, Ahmed; El-Zainy, Medhat

    2011-12-01

    In the present study, 40 adult male albino rats were used to study the effect of gamma radiation on the hard dental tissues (enamel surface, dentinal tubules and the cementum surface). The rats were irradiated at 0.2, 0.5, 1.0, 2.0, 4.0 and 6.0 Gy gamma doses. The effects of irradiated hard dental tissues samples were investigated using a scanning electron microscope. For doses up to 0.5 Gy, there was no evidence of the existence of cracks on the enamel surface. With 1 Gy irradiation dose, cracks were clearly observed with localized erosive areas. At 2 Gy irradiation dose, the enamel showed morphological alterations as disturbed prismatic and interprismatic areas. An increase in dentinal tubules diameter and a contemporary inter-tubular dentine volume decrease were observed with higher irradiation dose. Concerning cementum, low doses,<0.5 Gy, showed surface irregularities and with increase in the irradiation dose to≥1 Gy, noticeable surface irregularities and erosive areas with decrease in Sharpey's fiber sites were observed. These observations could shed light on the hazardous effects of irradiation fields to the functioning of the human teeth.

  13. Enablers of the implementation of tissue plasminogen activator in acute stroke care: a cross-sectional survey.

    Directory of Open Access Journals (Sweden)

    Alice Grady

    Full Text Available To assess emergency physicians' perceptions of individual and system enablers to the use of tissue Plasminogen Activator in acute stroke.Australian fellows and trainees of Australasian College for Emergency Medicine completed a 57-item online survey assessing enablers to implementation of evidence-based practice across six domains: knowledge, skills, modelling, monitoring, feedback, and maintenance. Demographic and workplace characteristics were obtained. Descriptive statistics were calculated to describe demographic and workplace characteristics of responders, and survey responses. Each domain received an overall score (% based on the number of responders agreeing with all items within the domain.A total of 429 (13% Australasian College for Emergency Medicine members responded. 17.7% of respondents reported they and/or their workplace met all knowledge-related enablers, however only 2.3% had all skill-related enablers in place. Of respondents who decide which patients receive tissue Plasminogen Activator treatment, 18.1% agreed that all maintenance-related enablers are in place at their hospital, compared to 6.6% for those who do not decide which patients receive tissue Plasminogen Activator treatment. None of the respondents had all items in place cross all domains.Even when allowing for the low response rate, it seems likely there is a lack of individual and system enablers supporting the implementation of best-practice stroke care in a number of Australian hospitals. Quality improvement programs could target all domains, particularly the skills-training and feedback emergency physicians receive, to aid implementation of tissue Plasminogen Activator treatment for acute stroke.

  14. A Novel Hybrid Ultramicrotomy/FIB-SEM Technique: Preparation of Serial Electron-Transparent Thin Sections of a Hayabusa Grain

    Science.gov (United States)

    Berger, Eve L.; Keller, Lindsay P.

    2014-01-01

    The Japanese space agency's (JAXA) Hayabusa mission returned the first particulate samples (typically grain surfaces and interiors. Using this method, we increase the number of FIB-prepared sections that can be recovered from a particle with dimensions on the order of tens of microns. These sections can be subsequently analyzed using a variety of analytical techniques. Particle RA-QD02-0211 is a approx. 40×40×20 micron particle from Itokawa containing olivine and Fe sulfides. It was embedded in low viscosity epoxy and partly sectioned to a depth of approx 10 micron; sections are placed on Cu grids with thin amorphous films for transmission electron microscope (TEM) analyses. With the sample surface partly exposed, the epoxy bullet is trimmed to a height of approx. 5mm to accommodate the allowable dimensions for FIB work (FEI Quanta 600 3D dual beam FIB-SEM). Using a diamond trim knife, the epoxy surrounding the grain is removed on 3 sides (to within a few microns of the grain); the depth of material removed extends well below the bottom of the particle. The sample is attached to an SEM pin mount, the epoxy coated with conductive paint, and the entire assembly coated with approx. 40nm of carbon to eliminate sample charging during FIB work. A protective carbon cap is placed according to the plan for the 15 FIB sections. The central 'spine' of the cap runs perpendicular to the front of the sample, and the 'ribs' protruding from either side run parallel. Each rib indicates the location of a planned FIB section, and the spine contains the final two planned sections. We use a cap with a 4 micron-wide spine and 2micron-wide ribs that have ?3.5 micron of space between them (narrower cuts result in too much re-deposition of material inside the trenches). Using a 30kV, 3nA ion-beam we expose the front surface of the grain and commence milling trenches between sections. Rather than using the typical C-cut to prepare the sample for lift-out, an L-cut is used instead, leaving

  15. Electron microscopic studies of the matrix formation of hard tissue organized cell

    International Nuclear Information System (INIS)

    Chou, Ching-Eng

    1982-01-01

    In order to study the functions of odontoblast, especially on the matrix formation, odontoblasts of rats' incisor and molar teeth were used. The animals were sacrificed 5 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours and 24 hours after 3 H-proline administration to obtain the specimen. The specimens were processed for electron microscopic autoradiography. The following results were obtained. 1. 5 minutes after 3 H-proline administration: Silver grains indicated 3 H-proline uptake were already noted within the cells and localized in the rough surfaced endoplasmic reticulum and surrounding ribosomes, partially within the karyoplasm. 2. 15 minutes after 3 H-proline administration: The number of silver grains were generally increased as compared with the findings obtained in 5 minutes. The localization moved to the Golgi apparatus and their surroundings. 3. 30 minutes after 3 H-proline administration: Silver grains obtained in Tome's fibers area and some in predentin. In this area granules derived from Golgi body were found. 4. 1 hour after 3 H-proline administration: The number of silver grains were generally decreased and more pronounced movement toward predentin, the marked movement of silver grains were obtained onto the collagen fibers and surroundings. 5. 2 hours after 3 H-proline administration: Silver grains moved to the calcified area and there collagen fibers became more remarkable. 6. 24 hours after 3 H-proline administration: No silver grains were founded in the odontoblast side but deposited in the predentin calcified area with stable condition. Based on the results of these observations, odontoblasts were shown to perform the function of synthesis, storage, transportation and control of collagen formation in addition to the role of matrix formation. (author)

  16. Pharmacy Customers’ Experiences With Electronic Prescriptions: Cross-Sectional Survey on Nationwide Implementation in Finland

    Science.gov (United States)

    Timonen, Johanna; Ahonen, Riitta

    2018-01-01

    Background One of the forerunners in electronic health, Finland has introduced electronic prescriptions (ePrescriptions) nationwide by law. This has led to significant changes for pharmacy customers. Despite the worldwide ambition to develop ePrescription services, there are few reports of nationally adopted systems and particularly on the experiences of pharmacy customers. Objective The aim of this study was to investigate Finnish pharmacy customers’ (1) experiences with purchasing medicines with ePrescriptions; (2) experiences with renewing ePrescriptions and acting on behalf of someone else at the pharmacy; (3) ways in which customers keep up to date with their ePrescriptions; and (4) overall satisfaction with ePrescriptions. Methods Questionnaires were distributed to 2913 pharmacy customers aged ≥18 years purchasing prescription medicines for themselves with an ePrescription in 18 community pharmacies across Finland in autumn 2015. Customers’ experiences were explored with 10 structured questions. The data were stored in SPSS for Windows and subjected to descriptive analysis, chi-square, Fisher exact, Kolmogorov-Smirnov, the Mann-Whitney U, and Kruskal-Wallis tests. Results Completed questionnaires were returned by 1288 customers, a response rate of 44.19% (1288/2913). The majority of the respondents did not encounter any problems during pharmacy visits (1161/1278, 90.85%) and were informed about the current status of their ePrescriptions after their medication was dispensed (1013/1276, 79.44%). Over half of the respondents had usually received a patient instruction sheet from their physician (752/1255, 59.92%), and nearly all of them regarded its content as clear (711/724, 98.2%). Half of the respondents had renewed their ePrescriptions through the pharmacy (645/1281, 50.35%), and one-third of them had acted on behalf of someone else with ePrescriptions (432/1280, 33.75%). Problems were rarely encountered in the renewal process (49/628, 7.8%) or when

  17. Pharmacy Customers' Experiences With Electronic Prescriptions: Cross-Sectional Survey on Nationwide Implementation in Finland.

    Science.gov (United States)

    Lämsä, Elina; Timonen, Johanna; Ahonen, Riitta

    2018-02-23

    One of the forerunners in electronic health, Finland has introduced electronic prescriptions (ePrescriptions) nationwide by law. This has led to significant changes for pharmacy customers. Despite the worldwide ambition to develop ePrescription services, there are few reports of nationally adopted systems and particularly on the experiences of pharmacy customers. The aim of this study was to investigate Finnish pharmacy customers' (1) experiences with purchasing medicines with ePrescriptions; (2) experiences with renewing ePrescriptions and acting on behalf of someone else at the pharmacy; (3) ways in which customers keep up to date with their ePrescriptions; and (4) overall satisfaction with ePrescriptions. Questionnaires were distributed to 2913 pharmacy customers aged ≥18 years purchasing prescription medicines for themselves with an ePrescription in 18 community pharmacies across Finland in autumn 2015. Customers' experiences were explored with 10 structured questions. The data were stored in SPSS for Windows and subjected to descriptive analysis, chi-square, Fisher exact, Kolmogorov-Smirnov, the Mann-Whitney U, and Kruskal-Wallis tests. Completed questionnaires were returned by 1288 customers, a response rate of 44.19% (1288/2913). The majority of the respondents did not encounter any problems during pharmacy visits (1161/1278, 90.85%) and were informed about the current status of their ePrescriptions after their medication was dispensed (1013/1276, 79.44%). Over half of the respondents had usually received a patient instruction sheet from their physician (752/1255, 59.92%), and nearly all of them regarded its content as clear (711/724, 98.2%). Half of the respondents had renewed their ePrescriptions through the pharmacy (645/1281, 50.35%), and one-third of them had acted on behalf of someone else with ePrescriptions (432/1280, 33.75%). Problems were rarely encountered in the renewal process (49/628, 7.8%) or when acting on behalf of another person (25

  18. Electronic cigarette use among adolescents: a cross-sectional study in Hong Kong

    Directory of Open Access Journals (Sweden)

    Nan Jiang

    2016-03-01

    Full Text Available Abstract Background Little is known about electronic cigarette (e-cigarette use among Chinese adolescents. We examined the prevalence of current (past 30-day e-cigarette use and its associated factors in a large sample of adolescents in Hong Kong. Methods We analyzed data of the School-based Survey on Smoking among Students 2012/13 from a representative sample of 45,857 secondary school students (mean age: 14.8 ± 1.9. We conducted chi-square tests and t-test to compare current e-cigarette use by covariates. We used multivariable logistic regression to examine the association between current e-cigarette use and demographic variables, parental smoking, peer smoking, knowledge about the harm of cigarette smoking, attitudes toward cigarette smoking, cigarette smoking status, use of other tobacco products, and alcohol consumption. Results Overall, 1.1 % of students reported current e-cigarette use. Of e-cigarette users, 11.7 % were never-cigarette smokers, 15.8 % were experimental cigarette smokers, 39.3 % were former cigarette smokers, and 33.2 % were current cigarette smokers. Current e-cigarette use was associated with male sex, poor knowledge about the harm of smoking, cigarette smoking, use of other tobacco products, and alcohol consumption. Conclusions Surveillance and intervention efforts should address a wide range of tobacco products, including e-cigarettes. Tobacco cessation programs should also address alcohol use collectively. Policies prohibiting e-cigarette sales to minors may help prevent e-cigarette uptake among adolescents.

  19. Characterization of printed pigment-based inks on ink-jet media using cross-sectional electron microscopy

    International Nuclear Information System (INIS)

    Almeida, P. de; Pataki, T.; Peeters, D.; Roost, C. van

    2004-01-01

    This paper reports on the microscopic assessment of representative specimen cross-sections prepared by microtomy and ultramicrotomy with emphasis in structure-property information using optical, scanning and transmission electron microscopy, namely, the absolute optical density δ, the measured effective printing coverage C-tilde, the averaged pigment-based ink layer thickness t-bar, and the morphology at 100% nominal printing coverage. This work shows that for different test patches printed at the same nominal printing coverage a number of different printing schemes yield a pre-defined absolute optical density δ which basically depends on the measured effective printing coverage C-tilde and the type of pigment-based inks used (spectral absorptivity m≠∞) and therefore on the averaged pigment-based ink layer thickness t-bar. A method for estimating the spectral absorptivity m is presented which combines the absolute optical density δ of the test patch and the averaged pigment-based ink layer thickness t-bar as measured from cross-sectional electron microscopy

  20. The use of radiation trapping in the measurement of the electron excitation cross section for the production of the 1s4 (3P1) level of Ne

    International Nuclear Information System (INIS)

    Miers, R.E.; Gastineau, J.E.; Phillps, M.H.; Anderson, L.W.; Lin, C.C.

    1981-01-01

    The authors report the use of laser induced fluorescence for the first measurement of the electron excitation cross section for the production of the 1s 4 ( 3 P 1 ) level of Ne. Radiation trapping is used to lengthen the effective lifetime of the 1s 4 level allowing for the electron excitation cross section of the 1s 4 level to be measured in a manner similar to the measurement of electron excitation cross sections of the metastable 1s 3 and 1s 5 levels. (Auth.)

  1. Electron-impact rotationally elastic total cross sections for H2CO and HCOOH over a wide range of incident energy (0.01-2000 eV)

    International Nuclear Information System (INIS)

    Vinodkumar, Minaxi; Bhutadia, Harshad; Antony, Bobby; Mason, Nigel

    2011-01-01

    This paper reports computational results of the total cross sections for electron impact on H 2 CO and HCOOH over a wide range of electron impact energies from 0.01 eV to 2 keV. The total cross section is presented as sum of the elastic and electronic excitation cross sections for incident energies. The calculation uses two different methodologies, below the ionization threshold of the target the cross section is calculated using the UK molecular R-matrix code through the Quantemol-N software package while cross sections at higher energies are evaluated using the spherical complex optical potential formalism. The two methods are found to be consistent at the transition energy (∼15 eV). The present results are, in general, found to be in good agreement with previous experimental and theoretical results (wherever available) and, thus, the present results can serve as a benchmark for the cross section over a wide range of energy.

  2. Single electron ionization and electron capture cross sections for (C{sup 6+}, H{sub 2}O) interaction within the Classical Trajectory Monte Carlo (CTMC) approach

    Energy Technology Data Exchange (ETDEWEB)

    Tran, H.N., E-mail: tranngochoang@tdt.edu.vn [Division of Nuclear Physics, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); Faculty of Applied Sciences, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); Dao, D.D. [Division of Nuclear Physics, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); Faculty of Applied Sciences, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); Incerti, S. [Division of Nuclear Physics, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); Faculty of Applied Sciences, Ton Duc Thang University, Tan Phong Ward, District 7, Ho Chi Minh City (Viet Nam); CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan (France); Université de Bordeaux, CENBG, UMR 5797, F-33170 Gradignan (France); Bernal, M.A. [Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, SP (Brazil); Karamitros, M. [CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan (France); Université de Bordeaux, CENBG, UMR 5797, F-33170 Gradignan (France); Nhan Hao, T.V. [Center of Research and Development, Duy Tan University, K7/25 Quang Trung, Danang (Viet Nam); Center for Theoretical and Computational Physics, College of Education, Hue University, 34 Le Loi Street, Hue City (Viet Nam); Dang, T.M. [VNUHCM-University of Science (Viet Nam); Francis, Z. [Saint Joseph University, Beyrouth (Lebanon)

    2016-01-01

    In this work, we present a derivation of cross sections for single ionization and electron capture processes within the Classical Trajectory Monte Carlo (CTMC) approach. Specifically, we have used a potential stemming from an ab initio calculation in Green et al.’s framework to describe the dynamics of the water molecule system. Proposing a modified version of the Classical Over-Barrier (COB) potential, we have found that a cut-off of roughly 28 a.u. on the initial distance of the projectile produced a reasonable accuracy. A global agreement has been obtained in our calculations compared to experimental and other theoretical results for C{sup 6+} ion energies ranging from 10 keV/u to 10 MeV/u.

  3. Synovial fluid white cell count and histopathological examination of periprosthetic tissue samples (frozen and permanent sections in the diagnosis of prosthetic knee infection

    Directory of Open Access Journals (Sweden)

    Obada B.

    2017-02-01

    Full Text Available The aim of the study was to determine prospectively the importance of synovial fluid white cell count and intraoperative frozen and permanent sections analysis (number of polymorphonuclear leukocytes per high-power field in the diagnosis of septic total knee arthroplasty. There were studied prospectively 72 patients who needed a revision total knee arthroplasty between 2013-2015. 30 patients were diagnosed with prosthetic joint infection due to high rates of ESR (93% and CRP (90% and preoperative positive culture from aspirated synovial fluid and 42 patients were considered to have aseptic failure according to negative preoperative culture from joint aspirate. For all the patients was analysed synovial fluid white cell count and histopathological aspect of intraoperative frozen and permanent sections of periprosthetic tissue. The results showed a median value of 13800 of sinovial white cells count for infected knee and 92 for noninfected knee. 90% of the patients with joint infection had more than 5 polymorphonuclear leukocytes per high power field on intraoperative frozen sections and 83% on permanent sections. None of the patients from aseptic group had more than 5 polymorphonuclear leukocytes per field on permanent sections. The erythrocyte sedimentation rate and C-reactive protein level can be supplemented with cultures of aspirated joint fluid and fluid white cell count to confirm the diagnosis of periprosthetic infection. When the preoperative diagnosis remain unclear, the histological examination of frozen or permanent sections of periprosthetic tissue with at least 5 polymorphonuclear leukocytes per high power field, is predictive for the presence of infection.

  4. Influence of the Coulomb interaction in the final state on the cross section of single-electron capture by fast ions

    International Nuclear Information System (INIS)

    Novikov, N.V.; Teplova, Ya.A.

    2011-01-01

    It is shown that the Coulomb interaction of ions in the final state must be taken into account in the estimation of the cross section of electron capture by fast ions. The cross section of electron capture decreases considerably, and the dependence of the cross section on the collision energy becomes close to the experimental one if the interaction of charged particles after collision is taken into account. -- Highlights: → Coulomb interaction of ions in the final state must be taken into account. → This interaction leads to a considerable decrease in the cross section. → The dependence on energy close to the experimental one.

  5. Calculations of elastic, ionization and total cross sections for inert gases upon electron impact: threshold to 2 keV

    Energy Technology Data Exchange (ETDEWEB)

    Vinodkumar, Minaxi [V P and R P T P Science College, Vallabh Vidyanagar 388 120, Gujarat (India); Limbachiya, Chetan [P S Science College, Kadi 382 715, Gujarat (India); Antony, Bobby [Department of Environmental, Earth and Atmospheric Sciences, University of Massachusetts Lowell, 265 Riverside Street, Lowell, MA 01854-5045 (United States); Joshipura, K N [Department of Physics, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat (India)

    2007-08-28

    In this paper we report comprehensive calculations of total elastic (Q{sub el}), total ionization (Q{sub ion}) and total (complete) cross sections (Q{sub T}) for the impact of electrons on inert gases (He, Ne, Ar, Kr and Xe) at energies from about threshold to 2000 eV. We have employed the spherical complex optical potential (SCOP) formalism to evaluate Q{sub el} and Q{sub T} and used the complex spherical potential-ionization contribution (CSP-ic) method to derive Q{sub ion}. The dependence of Q{sub T} on polarizability and incident energy is presented for these targets through an analytical formula. Mutual comparison of various cross sections is provided to show their relative contribution to the total cross sections Q{sub T}. Comparison of Q{sub T} for all these targets is carried out to present a general theoretical picture of collision processes. The present calculations also provide information, hitherto sparse, on the excitation processes of these atomic targets. These results are compared with available experimental and other theoretical data and overall good agreement is observed.

  6. Supervised Mineral Classification with Semi-automatic Training and Validation Set Generation in Scanning Electron Microscope Energy Dispersive Spectroscopy Images of Thin Sections

    DEFF Research Database (Denmark)

    Flesche, Harald; Nielsen, Allan Aasbjerg; Larsen, Rasmus

    2000-01-01

    This paper addresses the problem of classifying minerals common in siliciclastic and carbonate rocks. Twelve chemical elements are mapped from thin sections by energy dispersive spectroscopy in a scanning electron microscope (SEM). Extensions to traditional multivariate statistical methods...

  7. Measurement of the cross-section of electron-positron scattering at high energy and quantum electrodynamics testing; Mesure de la section efficace de diffusion electron-positron a haute energie et validite de l'electrodynamique quantique

    Energy Technology Data Exchange (ETDEWEB)

    Lalanne, D.

    1970-07-17

    The experiment we have performed on the ACO (Orsay Collider Ring) is one of the most accurate tests of quantum electrodynamics over very short interaction distances (10{sup -14} cm). We have studied the electron-positron elastic scattering at very wide angle. This work is divided into 4 parts. The first part reviews recent tests of quantum electrodynamics and presents the electron-positron elastic scattering. The second part describes the measurement of brightness: the experimental device, data analysis and accuracy. The measurement of brightness has been performed by detecting the photons emitted in the double Bremsstrahlung reaction: e{sup +}e{sup -} → e{sup +}e{sup -}γγ. The third part deals with the measurement of the number of Bhabha events. The last part compares the experimental value of the Bhabha scattering with the theoretically expected value. We have got the following results: the number of Bhabha events: 757 events, the experimental value for Bhabha scattering cross-section: [1.97 ± 0.09 (stat.) ± 0.10 (syst.)]*10{sup -31} cm{sup 2}. The comparison of this experimental value with the expected value has allowed us to set the lower limit of the cutting parameter Λ: Λ > 2 GeV.

  8. Ab initio calculation of the cross sections for electron impact vibrational excitation of CO via the {sup 2}Π shape resonance

    Energy Technology Data Exchange (ETDEWEB)

    Falcetta, Michael F., E-mail: mffalcetta@gcc.edu; Fair, Mark C.; Tharnish, Emily M.; Williams, Lorna M.; Hayes, Nathan J. [Department of Chemistry, Grove City College, Grove City, Pennsylvania 16127 (United States); Jordan, Kenneth D. [Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 (United States)

    2016-03-14

    The stabilization method is used to calculate the complex potential energy curve of the {sup 2}Π state of CO{sup −} as a function of bond length, with the refinement that separate potentials are determined for p-wave and d-wave attachment and detachment of the excess electron. Using the resulting complex potentials, absolute vibrational excitation cross sections are calculated as a function of electron energy and scattering angle. The calculated cross sections agree well with experiment.

  9. An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing.

    Science.gov (United States)

    Amini, Parisa; Ettlin, Julia; Opitz, Lennart; Clementi, Elena; Malbon, Alexandra; Markkanen, Enni

    2017-08-23

    Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA-protein crosslinking and RNA fragmentation, both of which heavily impact on RNA quantity and quality for downstream analysis. With very small sample sizes, e.g. when performing Laser-capture microdissection (LCM) to isolate specific subpopulations of cells, recovery of sufficient RNA for analysis with reverse-transcription quantitative PCR (RT-qPCR) or next-generation sequencing (NGS) becomes very cumbersome and difficult. We excised matched cancer-associated stroma (CAS) and normal stroma from clinical specimen of FFPE canine mammary tumours using LCM, and compared the commonly used protease-based RNA isolation procedure with an adapted novel technique that additionally incorporates a focused ultrasonication step. We successfully adapted a protocol that uses focused ultrasonication to isolate RNA from small amounts of deparaffinised, stained, clinical LCM samples. Using this approach, we found that total RNA yields could be increased by 8- to 12-fold compared to a commonly used protease-based extraction technique. Surprisingly, RNA extracted using this new approach was qualitatively at least equal if not superior compared to the old approach, as Cq values in RT-qPCR were on average 2.3-fold lower using the new method. Finally, we demonstrate that RNA extracted using the new method performs comparably in NGS as well. We present a successful isolation protocol for extraction of RNA from difficult and limiting FFPE tissue samples that enables successful analysis of small sections of clinically relevant specimen. The possibility to study gene expression signatures in specific small sections of archival FFPE tissue, which often entail large amounts of highly relevant clinical follow-up data, unlocks a new dimension of hitherto difficult-to-analyse samples which now

  10. Plural three-wave resonances of space charge wave harmonics in transit section of klystron-type two-stream FEL with helical electron beam

    DEFF Research Database (Denmark)

    Lysenko, Alexander; Volk, Iurii; Serozhko, Anastasia

    2017-01-01

    We have carried out the research of plural three-wave resonances of space charge wave (SCW) harmonics in the transit section of the klystron type two-stream superheterodyne free-electron laser (TSFEL) with helical electron beam in cubic non-linear approximation. We have found out that two...

  11. Coplanar asymmetric angles and symmetric energy sharing triple differential cross sections for 200 eV electron-impact ionization of Ar (3p)

    International Nuclear Information System (INIS)

    Ozer, Zehra N; Varol, Onur; Yavuz, Murat; Dogan, Mevlut; Amami, Sadek; Madison, Don

    2015-01-01

    We have measured triple differential cross sections (TDCSs) for electron-impact ionization of the 3p shell of Ar at 200 eV incident electron energy. The experiments have been performed in coplanar asymmetric energy sharing geometry. The experimental results are compared with the theoretical models of three body distorted wave (3DW) and distorted wave Born approximation (DWBA). (paper)

  12. MO-F-CAMPUS-J-04: Tissue Segmentation-Based MR Electron Density Mapping Method for MR-Only Radiation Treatment Planning of Brain

    Energy Technology Data Exchange (ETDEWEB)

    Yu, H [Sunnybrook Health Sciences Centre, Toronto, Ontario (Canada); Lee, Y [Sunnybrook Odette Cancer Centre, Toronto, Ontario (Canada); Ruschin, M [Odette Cancer Centre, Toronto, ON (Canada); Karam, I [Sunnybrook Odette Cancer Center, Toronto, Ontario (Canada); Sahgal, A [University of Toronto, Toronto, ON (Canada)

    2015-06-15

    Purpose: Automatically derive electron density of tissues using MR images and generate a pseudo-CT for MR-only treatment planning of brain tumours. Methods: 20 stereotactic radiosurgery (SRS) patients’ T1-weighted MR images and CT images were retrospectively acquired. First, a semi-automated tissue segmentation algorithm was developed to differentiate tissues with similar MR intensities and large differences in electron densities. The method started with approximately 12 slices of manually contoured spatial regions containing sinuses and airways, then air, bone, brain, cerebrospinal fluid (CSF) and eyes were automatically segmented using edge detection and anatomical information including location, shape, tissue uniformity and relative intensity distribution. Next, soft tissues - muscle and fat were segmented based on their relative intensity histogram. Finally, intensities of voxels in each segmented tissue were mapped into their electron density range to generate pseudo-CT by linearly fitting their relative intensity histograms. Co-registered CT was used as a ground truth. The bone segmentations of pseudo-CT were compared with those of co-registered CT obtained by using a 300HU threshold. The average distances between voxels on external edges of the skull of pseudo-CT and CT in three axial, coronal and sagittal slices with the largest width of skull were calculated. The mean absolute electron density (in Hounsfield unit) difference of voxels in each segmented tissues was calculated. Results: The average of distances between voxels on external skull from pseudo-CT and CT were 0.6±1.1mm (mean±1SD). The mean absolute electron density differences for bone, brain, CSF, muscle and fat are 78±114 HU, and 21±8 HU, 14±29 HU, 57±37 HU, and 31±63 HU, respectively. Conclusion: The semi-automated MR electron density mapping technique was developed using T1-weighted MR images. The generated pseudo-CT is comparable to that of CT in terms of anatomical position of

  13. Advisory Committee for the Calibration Standards of Ionizing Radiation Measurement. Section 1. X and #betta# radiations, electrons

    International Nuclear Information System (INIS)

    1982-01-01

    Section I (Rayons X et #betta#, electrons) of the Comite Consultatif pour les Etalons de Mesure des Rayonnements Ionisants held its sixth meeting in June 1981. The work carried out recently in various laboratories represented at the meeting is contained in a series of Progress Reports. Several international comparisons of exposure standards performed at BIPM between 5 keV and 1.2 MeV were presented and the question of exposure measurements at protection-level exposure rates was raised. The work of the three existing Working Parties was discussed at length: comparison of Fricke chemical dosimetry systems, conversion of exposure to absorbed dose in water, conversion of graphite absorbed dose into water absorbed dose. A recommendation was made concerning the possibility of expressing in terms of air kerma or water kerma calibrations made in terms of exposure [fr

  14. Precise measurement of the left-right cross section asymmetry in Z boson production by electron-positron collisions

    Energy Technology Data Exchange (ETDEWEB)

    Frey, R.E. [Univ. of Oregon, Eugene, OR (United States)

    1994-12-01

    A precise measurement of the left-right cross section asymmetry (A{sub LR}) for Z boson production by e{sup +}e{sup {minus}} collisions has been attained at the SLAC Linear Collider with the SLD detector. The author describes this measurement for the 1993 data run, emphasizing the significant improvements in polarized beam operation which took place for this run, where the luminosity-weighted electron beam polarization averaged 62.6 {+-} 1.2%. Preliminary 1993 results for A{sub LR} are presented. When combined with the (less precise) 1992 result, the preliminary result for the effective weak mixing angle is sin{sup 2}{theta}{sub W}{sup eff} = 0.2290 {+-} 0.0010.

  15. Determination of the absolute two-photon ionization cross section of He by an XUV free electron laser

    International Nuclear Information System (INIS)

    Sato, Takahiro; Iwasaki, Atsushi; Ishibashi, Kazuki; Okino, Tomoya; Yamanouchi, Kaoru; Adachi, Junichi; Yagishita, Akira; Yazawa, Hiroki; Aoyma, Makoto; Yabashi, Makina; Nagasono, Mitsuru; Higashiya, Atsushi; Ishikawa, Tetsuya; Kannari, Fumihiko; Yamakawa, Koichi; Midorikawa, Katsumi; Nakano, Hidetoshi

    2011-01-01

    The resonant and non-resonant two-photon single ionization processes of He were investigated using intense free electron laser light in the extreme ultraviolet (XUV) region (53.4-61.4 nm) covering the 1s-2p and 1s-3p resonant transitions of He. On the basis of the dependences of the yield of He + on the XUV light-field intensity at 53.4, 58.4, 56.0 and 61.4 nm, the absolute values of the two-photon ionization cross sections of He at the four different wavelengths and their dependence on the light-field intensity were determined for the first time. (fast track communication)

  16. Study of the Reactive-element Effect in Oxidation of Fe-cr Alloys Using Transverse Section Analytical Electron Microscopy

    Science.gov (United States)

    King, W. E.; Ethridge, E. C.

    1985-01-01

    The role of trace additions of reactive elements like Y, Ce, Th, or Hf to Cr bearing alloys was studied by applying a new developed technique of transverse section analytical electron microscopy. This reactive-element effect improves the high temperature oxidation resistance of alloys by strongly reducing the high temperature oxidation rate and enhancing the adhesion of the oxide scale, however, the mechanisms for this important effect remain largely unknown. It is indicated that the presence of yttrium affects the oxidation of Fe-Cr-Y alloys in at least two ways. The reactive element alters the growth mechanism of the oxide scale as evidenced by the marked influence of the reactive element on the oxide scale microstructure. The present results also suggest that reactive-element intermetallic compounds, which internally oxidize in the metal during oxidation, act as sinks for excess vacancies thus inhibiting vacancy condensation at the scale-metal interface and possibly enhancing scale adhesion.

  17. Survival of plant tissue at super-low temperatures v. An electron microscope study of ice in cortical cells cooled rapidly.

    Science.gov (United States)

    Sakai, A; Otsuka, K

    1967-12-01

    Experiments were carried out with cortical cells in twig bark of mulberry trees in winter in order to clarify the mechanism of survival at super-low temperatures with rapid cooling and rewarming. Attention was given to the relation between the existence of intracellular ice crystals and survival.Cortical cells were cooled rapidly by direct immersion into liquid nitrogen or isopentane cooled at various temperatures. After immersion, they were freeze-substituted with absolute ethanol at -78 degrees . They were then embedded, sectioned and examined under the electron microscope for the presence and distribution of cavities left after ice removal.Cells were found to remain alive and contain no ice cavities when immersed rapidly into isopentane baths kept below -60 degrees . Those cells at intermediate temperatures from -20 degrees to -45 degrees , were almost all destroyed. It was also observed that many ice cavities were contained in the cells immersed rapidly into isopentane baths at -30 degrees . The data seem to indicate that no ice crystals were formed when cooled rapidly by direct immersion into isopentane baths below -60 degrees or into liquid nitrogen.The tissue sections immersed in liquid nitrogen were rapidly transferred to isopentane baths at temperatures ranging from -70 degrees to -10 degrees before rapid rewarming. There was little damage when samples were held at temperatures below -50 degrees for 10 minutes or below -60 degrees for 16 hours. No cavities were found in these cells. Above -45 degrees , and especially at -30 degrees , however, all cells were completely destroyed even when exposed only for 1 minute. Many ice cavities were observed throughout these cells. The results obtained may be explained in terms of the growth rate of intracellular ice crystals.

  18. Determination of the swelling velocity of different wood species and tissues depending on the cutting direction on microtome section level

    Science.gov (United States)

    Stuckenberg, Peter; Wenderdel, Christoph; Zauer, Mario

    2018-06-01

    Swelling velocity in dependence on the anatomical cutting direction of yew [Taxus baccata L.] and boxwood [Buxus sempervirens L.] was determined at temperature of 20 °C and at relative humidity of 10% and 100%. The investigations, conducted on a microtome section level, showed a similar behaviour for specimens of both wood species. It was possible to determine that the swelling velocity for yew and boxwood increases in its anatomical cutting directions. The longitudinal direction showed the lowest value, the tangential direction, by distinction, the highest value. Furthermore, a significant influence of early wood and late wood content on the swelling velocity for yew was detected.

  19. Dependence of secondary electron emission on surface charging in sapphire and polycrystalline alumina: Evaluation of the effective cross sections for recombination and trapping

    International Nuclear Information System (INIS)

    Said, K.; Damamme, G.; Si Ahmed, A.; Moya, G.; Kallel, A.

    2014-01-01

    Highlights: • A novel approach for the analysis of the secondary electron emission in connection with the surface density of trapped charges. • Experimental estimation of the effective cross section for electron–hole recombination and electron trapping in defects. • A simplified charge transport and trapping model which corroborates qualitatively the interpretation of the results. - Abstract: The evolution of the secondary electron emission from sapphire and polycrystalline alumina during electron irradiation, achieved in a scanning electron microscope at room temperature, is derived from the measurement of the induced and the secondary electron currents. The semi-logarithmic plot of the secondary electron emission yield versus the surface density of trapped charges displays a plateau followed by a linear variation. For positive charging, the slope of the linear part, whose value is of about 10 −9 cm 2 , is independent of the primary electron energy, the microstructure and the impurities. It is interpreted as an effective microscopic cross section for electron–hole recombination. For negative charging of sapphire, the slope is associated with an effective electron trapping cross section close to 10 −11 cm 2 , which can be assigned to the dominant impurity trap. These effective values reflect the multiple interactions leading to the accumulation of charges. The yield corresponding to the plateau is controlled by the initial density of impurity traps. A charge transport and trapping >model, based on simplifying assumptions, confirms qualitatively these inferences

  20. Measurement of the cross-section of electron-positron scattering at high energy and quantum electrodynamics testing

    International Nuclear Information System (INIS)

    Lalanne, D.

    1970-01-01

    The experiment we have performed on the ACO (Orsay Collider Ring) is one of the most accurate tests of quantum electrodynamics over very short interaction distances (10 -14 cm). We have studied the electron-positron elastic scattering at very wide angle. This work is divided into 4 parts. The first part reviews recent tests of quantum electrodynamics and presents the electron-positron elastic scattering. The second part describes the measurement of brightness: the experimental device, data analysis and accuracy. The measurement of brightness has been performed by detecting the photons emitted in the double Bremsstrahlung reaction: e + e - → e + e - γγ. The third part deals with the measurement of the number of Bhabha events. The last part compares the experimental value of the Bhabha scattering with the theoretically expected value. We have got the following results: the number of Bhabha events: 757 events, the experimental value for Bhabha scattering cross-section: [1.97 ± 0.09 (stat.) ± 0.10 (syst.)]*10 -31 cm 2 . The comparison of this experimental value with the expected value has allowed us to set the lower limit of the cutting parameter Λ: Λ > 2 GeV