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Sample records for thermotoga maritima solved

  1. Transcriptional regulation of the carbohydrate utilization network in Thermotoga maritima

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    Dmitry A Rodionov

    2013-08-01

    Full Text Available Hyperthermophilic bacteria from the Thermotogales lineage can produce hydrogen by fermenting a wide range of carbohydrates. Previous experimental studies identified a large fraction of genes committed to carbohydrate degradation and utilization in the model bacterium Thermotoga maritima. Knowledge of these genes enabled comprehensive reconstruction of biochemical pathways comprising the carbohydrate utilization network. However, transcriptional factors (TFs and regulatory mechanisms driving this network remained largely unknown. Here, we used an integrated approach based on comparative analysis of genomic and transcriptomic data for the reconstruction of the carbohydrate utilization regulatory networks in 11 Thermotogales genomes. We identified DNA-binding motifs and regulons for 19 orthologous TFs in the Thermotogales. The inferred regulatory network in T. maritima contains 181 genes encoding TFs, sugar catabolic enzymes and ABC-family transporters. In contrast to many previously described bacteria, a transcriptional regulation strategy of Thermotoga does not employ global regulatory factors. The reconstructed regulatory network in T. maritima was validated by gene expression profiling on a panel of mono- and disaccharides and by in vitro DNA-binding assays. The observed upregulation of genes involved in catabolism of pectin, trehalose, cellobiose, arabinose, rhamnose, xylose, glucose, galactose, and ribose showed a strong correlation with the UxaR, TreR, BglR, CelR, AraR, RhaR, XylR, GluR, GalR, and RbsR regulons. Ultimately, this study elucidated the transcriptional regulatory network and mechanisms controlling expression of carbohydrate utilization genes in T. maritima. In addition to improving the functional annotations of associated transporters and catabolic enzymes, this research provides novel insights into the evolution of regulatory networks in Thermotogales.

  2. Glycerol fermentation to hydrogen by Thermotoga maritima: Proposed pathway and bioenergetic considerations

    NARCIS (Netherlands)

    Maru, B.T.; Bielen, A.A.M.; Constanti, M.; Medina, F.; Kengen, S.W.M.

    2013-01-01

    The production of biohydrogen from glycerol, by the hyperthermophilic bacterium Thermotoga maritima DSM 3109, was investigated in batch and chemostat systems. T. maritima converted glycerol to mainly acetate, CO2 and H2. Maximal hydrogen yields of 2.84 and 2.41 hydrogen per glycerol were observed

  3. Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima

    NARCIS (Netherlands)

    Levisson, M.; Han, G.W.; Deller, M.C.; Hendriks, S.N.A.; Oost, van der J.; Kengen, S.W.M.

    2012-01-01

    TM0077 from Thermotoga maritima is a member of the carbohydrate esterase family 7 and is active on a variety of acetylated compounds, including cephalosporin C. TM0077 esterase activity is confined to short-chain acyl esters (C2-C3), and is optimal around 100°C and pH 7.5. The positional specificity

  4. Structural analysis of DNA sequence: evidence for lateral gene transfer in Thermotoga maritima

    DEFF Research Database (Denmark)

    Worning, Peder; Jensen, Lars Juhl; Nelson, K. E.

    2000-01-01

    The recently published complete DNA sequence of the bacterium Thermotoga maritima provides evidence, based on protein sequence conservation, for lateral gene transfer between Archaea and Bacteria. We introduce a new method of periodicity analysis of DNA sequences, based on structural parameters......, which brings independent evidence for the lateral gene transfer in the genome of T.maritima, The structural analysis relates the Archaea-like DNA sequences to the genome of Pyrococcus horikoshii. Analysis of 24 complete genomic DNA sequences shows different periodicity patterns for organisms...

  5. Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima

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    Mohammad S. Eram

    2015-12-01

    Full Text Available The data provide additional support of the characterization of the biophysical and biochemical properties of the enzyme acetohydroxyacid synthase from the hyperthermophilic bacterium Thermotoga maritima (Eram et al., 2015 [1]. The genes encoding the enzyme subunits have been cloned and expressed in the mesophilic host Escherichia coli. Detailed data include information about the optimization of the expression conditions, biophysical properties of the enzyme and reconstitution of the holoenzyme from individually expressed and purified subunits.

  6. Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima

    International Nuclear Information System (INIS)

    Lee, Hyung Ho

    2011-01-01

    Shikimate dehydrogenase from Thermotoga maritima has been overexpressed, crystallized, and its crystal structure has been determined. X-ray diffraction data have been collected to 1.45 Å. Shikimate dehydrogenase (SDH), which catalyses the NADPH-dependent reduction of 3-dehydroshikimate to shikimate in the shikimate pathway, is an attractive target for the development of herbicides and antimicrobial agents. Previous structural studies showed that SDH exists in two conformations, an open form and a closed form, and it is believed that the conformational state is crucial to understanding a catalytic mechanism. To facilitate further structural comparisons among SDHs, structural analysis of an SDH from Thermotoga maritima encoded by the Tm0346 gene has been initiated. SDH from T. maritima has been overexpressed in Escherichia coli and crystallized at 296 K using ammonium sulfate as a precipitant. Crystals of T. maritima SDH diffracted to 1.45 Å resolution and belonged to orthorhombic space group P2 1 2 1 2 1 , with unit-cell parameters a = 54.21, b = 62.45 and c = 68.68 Å. The asymmetric unit contains a monomer, with a corresponding V M of 2.01 Å 3 Da −1 and a solvent content of 38.9% by volume

  7. Enzymatic synthesis of c-di-GMP using inclusion bodies of Thermotoga maritima full-length diguanylate cyclase.

    Science.gov (United States)

    Korovashkina, A S; Rymko, A N; Kvach, S V; Zinchenko, A I

    2012-12-15

    Recombinant full-length diguanylate cyclases (DGCs) of Thermotoga maritima with native and mutant allosteric sites were overexpressed in Escherichia coli cells and characterized. It has been shown that target enzymes are produced substantially in the form of active inclusion bodies. Introduction of the mutation in allosteric site resulted in 7-fold increase of the T. maritima DGC activity. Possibility of applying full-length DGC of T. maritima in the form of inclusion bodies for synthesis of c-di-GMP was originally demonstrated. Copyright © 2012 Elsevier B.V. All rights reserved.

  8. Crystallization and preliminary crystallographic analysis of an esterase with a novel domain from the hyperthermophile Thermotoga maritima

    NARCIS (Netherlands)

    Sun, Lei; Levisson, Mark; Hendriks, Sjon; Akveld, Twan; Kengen, Serve W. M.; Dijkstra, Bauke W.; van der Oost, John

    A predicted esterase ( EstA) with an unusual new domain from the hyperthermophilic bacterium Thermotoga maritima has been cloned and overexpressed in Escherichia coli. The purified protein was crystallized by the hanging-drop vapour-diffusion technique in the presence of lithium sulfate and

  9. Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana.

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    Olszewski, Marcin; Grot, Anna; Wojciechowski, Marek; Nowak, Marta; Mickiewicz, Małgorzata; Kur, Józef

    2010-10-15

    In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods. We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino acid residues with a calculated molecular mass of 16.30 and 16.58 kDa, respectively. The similarity between amino acid sequences of these proteins is very high: 90% identity and 95% similarity. Surprisingly, both TmaSSB and TneSSB possess a quite low sequence similarity to Escherichia coli SSB (36 and 35% identity, 55 and 56% similarity, respectively). They are functional as homotetramers containing one single-stranded DNA binding domain (OB-fold) in each monomer. Agarose mobility assays indicated that the ssDNA-binding site for both proteins is salt independent, and fluorescence spectroscopy resulted in a size of 68 ± 2 nucleotides. The half-lives of TmaSSB and TneSSB were 10 h and 12 h at 100°C, respectively. When analysed by differential scanning microcalorimetry (DSC) the melting temperature (Tm) was 109.3°C and 112.5°C for TmaSSB and TneSSB, respectively. The results showed that TmaSSB and TneSSB are the most thermostable SSB proteins identified to date, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR).

  10. Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana

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    Mickiewicz Małgorzata

    2010-10-01

    Full Text Available Abstract Background In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods. Results We report the characterization of single-stranded DNA binding proteins (SSBs from the thermophilic bacteria Thermotoga maritima (TmaSSB and Thermotoga neapolitana (TneSSB. They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino acid residues with a calculated molecular mass of 16.30 and 16.58 kDa, respectively. The similarity between amino acid sequences of these proteins is very high: 90% identity and 95% similarity. Surprisingly, both TmaSSB and TneSSB possess a quite low sequence similarity to Escherichia coli SSB (36 and 35% identity, 55 and 56% similarity, respectively. They are functional as homotetramers containing one single-stranded DNA binding domain (OB-fold in each monomer. Agarose mobility assays indicated that the ssDNA-binding site for both proteins is salt independent, and fluorescence spectroscopy resulted in a size of 68 ± 2 nucleotides. The half-lives of TmaSSB and TneSSB were 10 h and 12 h at 100°C, respectively. When analysed by differential scanning microcalorimetry (DSC the melting temperature (Tm was 109.3°C and 112.5°C for TmaSSB and TneSSB, respectively. Conclusion The results showed that TmaSSB and TneSSB are the most thermostable SSB proteins identified to date, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR.

  11. Complexes of Thermotoga maritima S-adenosylmethionine decarboxylase provide insights into substrate specificity

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    Bale, Shridhar; Baba, Kavita; McCloskey, Diane E.; Pegg, Anthony E.; Ealick, Steven E.

    2010-06-25

    The polyamines putrescine, spermidine and spermine are ubiquitous aliphatic cations and are essential for cellular growth and differentiation. S-Adenosylmethionine decarboxylase (AdoMetDC) is a critical pyruvoyl-dependent enzyme in the polyamine-biosynthetic pathway. The crystal structures of AdoMetDC from humans and plants and of the AdoMetDC proenzyme from Thermotoga maritima have been obtained previously. Here, the crystal structures of activated T. maritima AdoMetDC (TmAdoMetDC) and of its complexes with S-adenosylmethionine methyl ester and 5{prime}-deoxy-5{prime}-dimethylthioadenosine are reported. The results demonstrate for the first time that TmAdoMetDC autoprocesses without the need for additional factors and that the enzyme contains two complete active sites, both of which use residues from both chains of the homodimer. The complexes provide insights into the substrate specificity and ligand binding of AdoMetDC in prokaryotes. The conservation of the ligand-binding mode and the active-site residues between human and T. maritima AdoMetDC provides insight into the evolution of AdoMetDC.

  12. Reactivation of methionine synthase from Thermotoga maritima (TM0268) requires the downstream gene product TM0269.

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    Huang, Sha; Romanchuk, Gail; Pattridge, Katherine; Lesley, Scott A; Wilson, Ian A; Matthews, Rowena G; Ludwig, Martha

    2007-08-01

    The crystal structure of the Thermotoga maritima gene product TM0269, determined as part of genome-wide structural coverage of T. maritima by the Joint Center for Structural Genomics, revealed structural homology with the fourth module of the cobalamin-dependent methionine synthase (MetH) from Escherichia coli, despite the lack of significant sequence homology. The gene specifying TM0269 lies in close proximity to another gene, TM0268, which shows sequence homology with the first three modules of E. coli MetH. The fourth module of E. coli MetH is required for reductive remethylation of the cob(II)alamin form of the cofactor and binds the methyl donor for this reactivation, S-adenosylmethionine (AdoMet). Measurements of the rates of methionine formation in the presence and absence of TM0269 and AdoMet demonstrate that both TM0269 and AdoMet are required for reactivation of the inactive cob(II)alamin form of TM0268. These activity measurements confirm the structure-based assignment of the function of the TM0269 gene product. In the presence of TM0269, AdoMet, and reductants, the measured activity of T. maritima MetH is maximal near 80 degrees C, where the specific activity of the purified protein is approximately 15% of that of E. coli methionine synthase (MetH) at 37 degrees C. Comparisons of the structures and sequences of TM0269 and the reactivation domain of E. coli MetH suggest that AdoMet may be bound somewhat differently by the homologous proteins. However, the conformation of a hairpin that is critical for cobalamin binding in E. coli MetH, which constitutes an essential structural element, is retained in the T. maritima reactivation protein despite striking divergence of the sequences.

  13. Structure of an essential bacterial protein YeaZ (TM0874) from Thermotoga maritima at 2.5 Å resolution

    International Nuclear Information System (INIS)

    Xu, Qingping; McMullan, Daniel; Jaroszewski, Lukasz; Krishna, S. Sri; Elsliger, Marc-André; Yeh, Andrew P.; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Carlton, Dennis; Chiu, Hsiu-Ju; Clayton, Thomas; Duan, Lian; Feuerhelm, Julie; Grant, Joanna; Han, Gye Won; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Reyes, Ron; Rife, Christopher L.; Bedem, Henry van den; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

    2009-01-01

    The crystal structure of an essential bacterial protein, YeaZ, from T. maritima identifies an interface that potentially mediates protein–protein interaction. YeaZ is involved in a protein network that is essential for bacteria. The crystal structure of YeaZ from Thermotoga maritima was determined to 2.5 Å resolution. Although this protein belongs to a family of ancient actin-like ATPases, it appears that it has lost the ability to bind ATP since it lacks some key structural features that are important for interaction with ATP. A conserved surface was identified, supporting its role in the formation of protein complexes

  14. Expression and Characterization of a Novel Nitrilase from Hyperthermophilic Bacterium Thermotoga maritima MSB8.

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    Chen, Zhi; Chen, Huayou; Ni, Zhong; Tian, Rui; Zhang, Tianxi; Jia, Jinru; Yang, Shengli

    2015-10-01

    The present study describes the gene cloning, overexpression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8. The nitrilase gene consisted of 804 base pairs, encoding a protein of 268 amino acid residues with a molecular mass of 30.07 kDa after SDS-PAGE analysis. The optimal temperature and pH of the purified enzyme were 45°C and 7.5, respectively. The enzyme demonstrated good temperature tolerance, with 40% residual activity after 60 min of heat treatment at 75°C. The kinetic constants Vmax and Km of this nitrilase toward 3-cyanopyridine were 3.12 μmol/min/mg and 7.63 mM, respectively. Furthermore, this novel nitrilase exhibited a broad spectrum toward the hydrolysis of the aliphatic nitriles among the tested substrates, and particularly was specific to aliphatic dinitriles like succinonitrile, which was distinguished from most nitrilases ever reported. The catalytic efficiency kcat/Km was 0.44 /mM/s toward succinonitrile. This distinct characteristic might enable this nitrilase to be a potential candidate for industrial applications for biosynthesis of carboxylic acid.

  15. Structural Insight inot the low Affinity Between Thermotoga maritima CheA and CheB Compared to their Escherichia coli/Salmonella typhimurium Counterparts

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    S Park; B Crane

    2011-12-31

    CheA-mediated CheB phosphorylation and the subsequent CheB-mediated demethylation of the chemoreceptors are important steps required for the bacterial chemotactic adaptation response. Although Escherichia coli CheB has been reported to interact with CheA competitively against CheY, we have observed that Thermotoga maritima CheB has no detectable CheA-binding. By determining the CheY-like domain crystal structure of T. maritima CheB, and comparing against the T. maritima CheY and Salmonella typhimurium CheB structures, we propose that the two consecutive glutamates in the {beta}4/{alpha}4 loop of T. maritima CheB that is absent in T. maritima CheY and in E. coli/S. typhimurium CheB may be one factor contributing to the low CheA affinity.

  16. Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration

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    Wang Yiran

    2009-06-01

    Full Text Available Abstract Background Thermostable enzymes from thermophilic microorganisms are playing more and more important roles in molecular biology R&D and industrial applications. However, over-production of recombinant soluble proteins from thermophilic microorganisms in mesophilic hosts (e.g. E. coli remains challenging sometimes. Results An open reading frame TM0438 from a hyperthermophilic bacterium Thermotoga maritima putatively encoding 6-phosphogluconate dehydrogenase (6PGDH was cloned and expressed in E. coli. The purified protein was confirmed to have 6PGDH activity with a molecular mass of 53 kDa. The kcat of this enzyme was 325 s-1 and the Km values for 6-phosphogluconate, NADP+, and NAD+ were 11, 10 and 380 μM, respectively, at 80°C. This enzyme had half-life times of 48 and 140 h at 90 and 80°C, respectively. Through numerous approaches including expression vectors, hosts, cultivation conditions, inducers, and codon-optimization of the 6pgdh gene, the soluble 6PGDH expression levels were enhanced to ~250 mg per liter of culture by more than 500-fold. The recombinant 6PGDH accounted for >30% of total E. coli cellular proteins when lactose was used as a low-cost inducer. In addition, this enzyme coupled with glucose-6-phosphate dehydrogenase for the first time was demonstrated to generate two moles of NADPH per mole of glucose-6-phosphate. Conclusion We have achieved a more than 500-fold improvement in the expression of soluble T. maritima 6PGDH in E. coli, characterized its basic biochemical properties, and demonstrated its applicability for NADPH regeneration by a new enzyme cocktail. The methodology for over-expression and simple purification of this thermostable protein would be useful for the production of other thermostable proteins in E. coli.

  17. Structure of a D-tagatose 3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima.

    Science.gov (United States)

    Sakuraba, Haruhiko; Yoneda, Kazunari; Satomura, Takenori; Kawakami, Ryushi; Ohshima, Toshihisa

    2009-03-01

    The crystal structure of a D-tagatose 3-epimerase-related protein (TM0416p) encoded by the hypothetical open reading frame TM0416 in the genome of the hyperthermophilic bacterium Thermotoga maritima was determined at a resolution of 2.2 A. The asymmetric unit contained two homologous subunits and a dimer was generated by twofold symmetry. The main-chain coordinates of the enzyme monomer proved to be similar to those of D-tagatose 3-epimerase from Pseudomonas cichorii and D-psicose 3-epimerase from Agrobacterium tumefaciens; however, TM0416p exhibited a unique solvent-accessible substrate-binding pocket that reflected the absence of an alpha-helix that covers the active-site cleft in the two aforementioned ketohexose 3-epimerases. In addition, the residues responsible for creating a hydrophobic environment around the substrate in TM0416p differ entirely from those in the other two enzymes. Collectively, these findings suggest that the substrate specificity of TM0416p is likely to differ substantially from those of other D-tagatose 3-epimerase family enzymes.

  18. Structure of a d-tagatose 3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima

    International Nuclear Information System (INIS)

    Sakuraba, Haruhiko; Yoneda, Kazunari; Satomura, Takenori; Kawakami, Ryushi; Ohshima, Toshihisa

    2009-01-01

    The crystal structure of a hyperthermophilic d-tagatose 3-epimerase-related protein with a unique active-site architecture was determined. The crystal structure of a d-tagatose 3-epimerase-related protein (TM0416p) encoded by the hypothetical open reading frame TM0416 in the genome of the hyperthermophilic bacterium Thermotoga maritima was determined at a resolution of 2.2 Å. The asymmetric unit contained two homologous subunits and a dimer was generated by twofold symmetry. The main-chain coordinates of the enzyme monomer proved to be similar to those of d-tagatose 3-epimerase from Pseudomonas cichorii and d-psicose 3-epimerase from Agrobacterium tumefaciens; however, TM0416p exhibited a unique solvent-accessible substrate-binding pocket that reflected the absence of an α-helix that covers the active-site cleft in the two aforementioned ketohexose 3-epimerases. In addition, the residues responsible for creating a hydrophobic environment around the substrate in TM0416p differ entirely from those in the other two enzymes. Collectively, these findings suggest that the substrate specificity of TM0416p is likely to differ substantially from those of other d-tagatose 3-epimerase family enzymes

  19. Influences of Various Peptide Linkers on the Thermotoga maritima MSB8 Nitrilase Displayed on the Spore Surface of Bacillus subtilis.

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    Chen, Huayou; Chen, Zhi; Wu, Bangguo; Ullah, Jawad; Zhang, Tianxi; Jia, Jinru; Wang, Hongcheng; Tan, Tianwei

    2017-01-01

    In the present study, fusion genes composed of Thermotoga maritima MSB8 nitrilase and Bacillus subtilis 168 outer coat protein CotG were constructed with various peptide linkers and displayed on B. subtilis DB 403 spores. The successful display of CotG-nit fusion proteins on the spore surface of B. subtilis was verified by Western blot analysis and activity measurement. It was demonstrated that the fusion with linker GGGGSEAAAKGGGGS presented the highest thermal and pH stability, which is 2.67- and 1.9-fold of the fusion without linker. In addition, fusion with flexible linker (GGGGS)3 demonstrated better thermal and pH stability than fusions with linkers GGGGS and (GGGGS)2. Fusion with rigid linker (EAAAK) demonstrated better thermal stability than fusions with linkers (EAAAK)2 and (EAAAK)3. Fusions with linker (EAAAK)2 demonstrated better pH stability than fusions with linkers (EAAAK) and (EAAAK)3. In the presence of 1 mM dithiothreitol, 1% (v/v) sodium dodecyl sulfate, and 20% (v/v) ethanol, the optimal linkers of the fusions were MGSSSN, GGGGSEAAAKGGGGS, and (GGGGS)3, respectively. In summary, our results showed that optimizing the peptide linkers with different type, length, and amino acid composition of the fusion proteins would be an efficient way to maintain the stability of fusion proteins and thus improve the nitrilase display efficiency, which could provide an effective method for rational design peptide linkers of displayed nitrilase on B. subtilis. © 2017 S. Karger AG, Basel.

  20. Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.

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    Drexler, David Jan; Müller, Martina; Rojas-Cordova, Carlos Alberto; Bandera, Adrian Maurice; Witte, Gregor

    2017-12-05

    The concentration of messenger molecules in bacterial cells needs to be tightly regulated. This can be achieved by either controlling the synthesis rate, degradation, or export by specific transporters, respectively. The regulation of the essential second messenger c-di-AMP is achieved by modulation of the diadenylate cyclase activity as well as by specific phosphodiesterases that hydrolyze c-di-AMP in the cell. We provide here structural and biochemical data on the DHH-type phosphodiesterase TmPDE (TM1595) from Thermotoga maritima. Our analysis shows that TmPDE is preferentially degrading linear dinucleotides, such as 5'-pApA, 5'-pGpG, and 5'-pApG, compared with cyclic dinucleotide substrates. The high-resolution structural data provided here describe all steps of the PDE reaction: the ligand-free enzyme, two substrate-bound states, and three post-reaction states. We can furthermore show that Pde2 from Streptococcus pneumoniae shares both structural features and substrate specificity based on small-angle X-ray scattering data and biochemical assays. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Cytoplasmic expression of a thermostable invertase from Thermotoga maritima in Lactococcus lactis.

    Science.gov (United States)

    Pek, Han Bin; Lim, Pei Yu; Liu, Chengcheng; Lee, Dong-Yup; Bi, Xuezhi; Wong, Fong Tian; Ow, Dave Siak-Wei

    2017-05-01

    To evaluate the secretory and cytoplasmic expression of a thermostable Thermogata maritima invertase in Lactococcus lactis. The thermostable invertase from T. maritima was cloned with and without the USP45 secretory peptide into the pNZ8148 vector for nisin-inducible expression in L. lactis. The introduction of an USP45 secretion peptide at the N-terminal of the enzyme led to a loss of protein solubility. Computational homology modeling and hydrophobicity studies indicated that the USP45 peptide exposes a stretch of hydrophobic amino acids on the protein surface resulting in lower solubility. Removal of the USP45 secretion peptide allowed a soluble and functional invertase to be expressed intracellularly in L. lactis. Immobilized metal affinity chromatography purification of the cell lysate with nickel-NTA gave a single protein band on SDS-PAGE, while E. coli-expressed invertase consistently co-purified with an additional band. The yields of the purified invertase from E. coli and L. lactis were 14.1 and 6.3 mg/l respectively. Invertase can be expressed in L. lactis and purified in a functional form. L. lactis is a suitable host for the production of food-grade invertase for use in the food and biotechnology industries.

  2. Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima

    Energy Technology Data Exchange (ETDEWEB)

    Barbey, Carole, E-mail: carole.barbey@smbh.univ-paris13.fr [Laboratoire de Biophysique Moléculaire, Cellulaire et Tissulaire, UMR 7033, Université Paris 13, UFR SMBH, 74 Rue Marcel Cachin, 93017 Bobigny CEDEX (France); Rouhier, Nicolas [Unité Mixte de Recherches 1136 INRA UHP (Interaction Arbres Microorganismes), IFR 110, Nancy Université BP 239, 54506 Vandoeuvre-lès-Nancy CEDEX (France); Haouz, Ahmed [Plate-forme de Cristallogenèse et Diffraction des Rayons X, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris (France); Navaza, Alda [Laboratoire de Biophysique Moléculaire, Cellulaire et Tissulaire, UMR 7033, Université Paris 13, UFR SMBH, 74 Rue Marcel Cachin, 93017 Bobigny CEDEX (France); Jacquot, Jean-Pierre [Unité Mixte de Recherches 1136 INRA UHP (Interaction Arbres Microorganismes), IFR 110, Nancy Université BP 239, 54506 Vandoeuvre-lès-Nancy CEDEX (France); Laboratoire de Biophysique Moléculaire, Cellulaire et Tissulaire, UMR 7033, Université Paris 13, UFR SMBH, 74 Rue Marcel Cachin, 93017 Bobigny CEDEX (France)

    2008-01-01

    Crystals of the peroxiredoxin domain of a larger natural hybrid protein from T. maritima were obtained which diffracted to 2.9 Å resolution on a synchrotron source. Thermotoga maritima contains a natural hybrid protein constituted of two moieties: a peroxiredoxin domain at the N-terminus and a nitroreductase domain at the C-terminus. The peroxiredoxin (Prx) domain has been overproduced and purified from Escherichia coli cells. The recombinant Prx domain, which is homologous to bacterial Prx BCP and plant Prx Q, folds properly into a stable protein that possesses biological activity. The recombinant protein was crystallized and synchrotron data were collected to 2.9 Å resolution. The crystals belonged to the tetragonal space group I422, with unit-cell parameters a = b = 176.67, c = 141.20 Å.

  3. The temperature dependence of the kinetic isotope effects of dihydrofolate reductase from Thermotoga maritima is influenced by intersubunit interactions.

    Science.gov (United States)

    Loveridge, E Joel; Allemann, Rudolf K

    2010-06-29

    Dihydrofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) is unique among structurally characterized chromosomal DHFRs in that it forms a stable homodimer. Dimerization is believed to play a key role in the high thermal stability of TmDHFR, which is reflected in a melting temperature in excess of 85 degrees C. The dimer interface of TmDHFR is composed of a hydrophobic core with charged residues around the periphery. In particular, Lys129 of each subunit forms three-membered salt bridges with Glu136 and Glu138 of the other subunit. To probe the role of these salt bridges in the dimerization and thermal stability of TmDHFR, we generated a series of variants (TmDHFR-K129E, TmDHFR-E136K, TmDHFR-E138K, and TmDHFR-E136K/E138K) in which these residues were exchanged for residues whose side chains bear the opposite charge. Our results indicate that these salt bridges are key for the high thermal stability of TmDHFR but are not a requirement for dimerization. Although the rate of dihydrofolate reduction by TmDHFR is not significantly affected by the loss of the K129-E136-E138 salt bridges, changes to the temperature dependence of the kinetic isotope effect on hydride transfer are observed. These changes are in agreement with the proposal that DHFR catalysis may be affected by changes to the conformational ensemble of the enzyme rather than only to the coupling of protein motions to the reaction coordinate.

  4. Structural and thermodynamic studies on a salt-bridge triad in the NADP-binding domain of glutamate dehydrogenase from Thermotoga maritima: cooperativity and electrostatic contribution to stability.

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    Lebbink, Joyce H G; Consalvi, Valerio; Chiaraluce, Roberta; Berndt, Kurt D; Ladenstein, Rudolf

    2002-12-31

    Cooperative interactions within ion-pair networks of hyperthermostable proteins are thought to be a major determinant for extreme protein stability. While the favorable thermodynamic contributions of optimized electrostatics in general as well as those of pairwise interactions have been documented, cooperativity between pairwise interactions has not yet been studied thermodynamically in proteins from hyperthermophiles. In this study we use the isolated cofactor binding domain of glutamate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima to analyze pairwise and cooperative interactions within the salt-bridge triad Arg190-Glu231-Lys193. The X-ray structure of the domain was solved at 1.43 A and reveals the salt-bridge network with surrounding solvent molecules in detail. All three participating charges in the network were mutated to alanine in all combinations. The X-ray structure of the variant lacking all three charges reveals that the removal of the side chains has no effect on the overall conformation of the protein. Using solvent denaturation and thermodynamic cycles, the interaction energies between each pair of residues in the network were determined in the presence and in the absence of the third residue. Both the Arg190-Glu231 ion pair and the Lys193-Glu231 salt bridge in the absence of the third residue, contribute favorably to the free energy for unfolding of the domain in urea. Using guanidinium chloride as denaturant reveals a strong cooperativity between the two ion-pair interactions, the presence of the second ion pair converts the first interaction from destabilizing into stabilizing by as much as 1.09 kcal/mol. The different energetics of the salt-bridge triad in urea and GdmCl are discussed with reference to the observed anion binding in the crystal structure at high ionic strength and their possible role in a highly charged, high-temperature environment such as the cytoplasm of hyperthermophiles.

  5. Contribution of Pentose Catabolism to Molecular Hydrogen Formation by Targeted Disruption of Arabinose Isomerase (araA) in the Hyperthermophilic Bacterium Thermotoga maritima

    Science.gov (United States)

    White, Derrick; Singh, Raghuveer; Rudrappa, Deepak; Mateo, Jackie; Kramer, Levi; Freese, Laura

    2016-01-01

    ABSTRACT Thermotoga maritima ferments a broad range of sugars to form acetate, carbon dioxide, traces of lactate, and near theoretic yields of molecular hydrogen (H2). In this organism, the catabolism of pentose sugars such as arabinose depends on the interaction of the pentose phosphate pathway with the Embden-Myerhoff and Entner-Doudoroff pathways. Although the values for H2 yield have been determined using pentose-supplemented complex medium and predicted by metabolic pathway reconstruction, the actual effect of pathway elimination on hydrogen production has not been reported due to the lack of a genetic method for the creation of targeted mutations. Here, a spontaneous and genetically stable pyrE deletion mutant was isolated and used as a recipient to refine transformation methods for its repair by homologous recombination. To verify the occurrence of recombination and to assess the frequency of crossover events flanking the deleted region, a synthetic pyrE allele, encoding synonymous nucleotide substitutions, was used. Targeted inactivation of araA (encoding arabinose isomerase) in the pyrE mutant was accomplished using a divergent, codon-optimized Thermosipho africanus pyrE allele fused to the T. maritima groES promoter as a genetic marker. Mutants lacking araA were unable to catabolize arabinose in a defined medium. The araA mutation was then repaired using targeted recombination. Levels of synthesis of H2 using arabinose-supplemented complex medium by wild-type and araA mutant cell lines were compared. The difference between strains provided a direct measurement of H2 production that was dependent on arabinose consumption. Development of a targeted recombination system for genetic manipulation of T. maritima provides a new strategy to explore H2 formation and life at an extremely high temperature in the bacterial domain. IMPORTANCE We describe here the development of a genetic system for manipulation of Thermotoga maritima. T. maritima is a

  6. Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80 degrees C: a model high temperature biocatalytic coating.

    Science.gov (United States)

    Lyngberg, Olav K; Solheid, Chris; Charaniya, Salim; Ma, Yue; Thiagarajan, Venkata; Scriven, L E; Flickinger, Michael C

    2005-06-01

    Thermostable polymers cast as thin, porous coatings or membranes may be useful for concentrating and stabilizing hyperthermophilic microorganisms as biocatalysts. Hydrogel matrices can be unstable above 65 degrees C. Therefore a 55-microm thick, two layer (cell coat + polymer top coat) bimodal, adhesive latex coating of partially coalesced polystyrene particles was investigated at 80 degrees C using Thermotoga maritima as a model hyperthermophile. Coating permeability (pore structure) was critical for maintaining T. maritima viability. The permeability of bimodal coatings generated from 0.8 v/v of a suspension of non-film-forming 800 nm polystyrene particles with high glass transition temperature (T(g) = 94 degrees C, 26.9% total solids) blended with 0.2 v/v of a suspension of film-forming 158 nm polyacrylate/styrene particles (T(g) approximately -5 degrees C, 40.9% total solids) with 0.3 g sucrose/g latex was measured in a KNO3 diffusion cell. Diffusivity ratio remained above 0.04 (D(eff)/D) when incubated at 80 degrees C in artificial seawater (ASW) for 5 days. KNO3 permeability was corroborated by cryogenic-SEM images of the pore structure. In contrast, the permeability of a mono-dispersed acrylate/vinyl acetate latex Rovace SF091 (T(g) approximately 10 degrees C) rapidly decreased and became impermeable after 2 days incubation in ASW at 80 degrees C. Thermotoga maritima were entrapped in these coatings at a cell density of 49 g cell wet weight/liter of coating volume, 25-fold higher than the density in liquid culture. Viable T. maritima were released from single-layer coatings at 80 degrees C but accurate measurement of the percentage of viable entrapped cells by plate counting was not successful. Metabolic activity could be measured in bilayer coatings by utilization of glucose and maltose, which was identical for latex-entrapped and suspended cells. Starch was hydrolyzed for 200 h by latex-entrapped cells due to the slow diffusion of starch through the

  7. Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains

    International Nuclear Information System (INIS)

    Zheng, Meiying; Cooper, David R.; Grossoehme, Nickolas E.; Yu, Minmin; Hung, Li-Wei; Cieslik, Marcin; Derewenda, Urszula; Lesley, Scott A.; Wilson, Ian A.; Giedroc, David P.; Derewenda, Zygmunt S.

    2009-01-01

    Here, the crystal structure of TM0439, a GntR regulator with an FCD domain found in the Thermotoga maritima genome, is described. The GntR superfamily of dimeric transcription factors, with more than 6200 members encoded in bacterial genomes, are characterized by N-terminal winged-helix DNA-binding domains and diverse C-terminal regulatory domains which provide a basis for the classification of the constituent families. The largest of these families, FadR, contains nearly 3000 proteins with all-α-helical regulatory domains classified into two related Pfam families: FadR-C and FCD. Only two crystal structures of FadR-family members, those of Escherichia coli FadR protein and LldR from Corynebacterium glutamicum, have been described to date in the literature. Here, the crystal structure of TM0439, a GntR regulator with an FCD domain found in the Thermotoga maritima genome, is described. The FCD domain is similar to that of the LldR regulator and contains a buried metal-binding site. Using atomic absorption spectroscopy and Trp fluorescence, it is shown that the recombinant protein contains bound Ni 2+ ions but that it is able to bind Zn 2+ with K d < 70 nM. It is concluded that Zn 2+ is the likely physiological metal and that it may perform either structural or regulatory roles or both. Finally, the TM0439 structure is compared with two other FadR-family structures recently deposited by structural genomics consortia. The results call for a revision in the classification of the FadR family of transcription factors

  8. Phenotypic Changes in Transgenic Tobacco Plants Overexpressing Vacuole-Targeted Thermotoga maritima BglB Related to Elevated Levels of Liberated Hormones

    Science.gov (United States)

    Nguyen, Quynh Anh; Lee, Dae-Seok; Jung, Jakyun; Bae, Hyeun-Jong

    2015-01-01

    The hyperthermostable β-glucosidase BglB of Thermotoga maritima was modified by adding a short C-terminal tetrapeptide (AFVY, which transports phaseolin to the vacuole, to its C-terminal sequence). The modified β-glucosidase BglB was transformed into tobacco (Nicotiana tabacum L.) plants. We observed a range of significant phenotypic changes in the transgenic plants compared to the wild-type (WT) plants. The transgenic plants had faster stem growth, earlier flowering, enhanced root systems development, an increased biomass biosynthesis rate, and higher salt stress tolerance in young plants compared to WT. In addition, programed cell death was enhanced in mature plants. Furthermore, the C-terminal AFVY tetrapeptide efficiently sorted T. maritima BglB into the vacuole, which was maintained in an active form and could perform its glycoside hydrolysis function on hormone conjugates, leading to elevated hormone [abscisic acid (ABA), indole 3-acetic acid (IAA), and cytokinin] levels that likely contributed to the phenotypic changes in the transgenic plants. The elevation of cytokinin led to upregulation of the transcription factor WUSCHELL, a homeodomain factor that regulates the development, division, and reproduction of stem cells in the shoot apical meristems. Elevation of IAA led to enhanced root development, and the elevation of ABA contributed to enhanced tolerance to salt stress and programed cell death. These results suggest that overexpressing vacuole-targeted T. maritima BglB may have several advantages for molecular farming technology to improve multiple targets, including enhanced production of the β-glucosidase BglB, increased biomass, and shortened developmental stages, that could play pivotal roles in bioenergy and biofuel production. PMID:26618153

  9. Constitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris.

    Science.gov (United States)

    Menéndez, Carmen; Martínez, Duniesky; Trujillo, Luis E; Mazola, Yuliet; González, Ernesto; Pérez, Enrique R; Hernández, Lázaro

    2013-02-01

    Enzymes for use in the sugar industry are preferred to be thermotolerant. In this study, a synthetic codon-optimized gene encoding a highly thermostable β-fructosidase (BfrA, EC 3.2.1.26) from the bacterium Thermotoga maritima was expressed in the yeast Pichia pastoris. The gradual increase of the transgene dosage from one to four copies under the control of the constitutive glyceraldehyde 3-phosphate dehydrogenase promoter had an additive effect on BfrA yield without causing cell toxicity. Maximal values of cell biomass (115 g/l, dry weight) and overall invertase activity (241 U/ml) were reached at 72 h in fed-batch fermentations using cane sugar as the main carbon source for growth. Secretion driven by the Saccharomyces cerevisiae α-factor signal peptide resulted in periplasmic retention (44 %) and extracellular release (56 %) of BfrA. The presence of N-linked oligosaccharides did not influence the optimal activity, thermal stability, kinetic properties, substrate specificity, and exo-type action mode of the yeast-secreted BfrA in comparison to the native unglycosylated enzyme. Complete inversion of cane sugar at initial concentration of 60 % (w/v) was achieved by periplasmic BfrA in undisrupted cells reacting at pH 5.5 and 70 °C, with average productivity of 4.4 g of substrate hydrolyzed per grams of biomass (wet weight) per hour. The high yield of fully active glycosylated BfrA here attained by recombinant P. pastoris in a low-cost fermentation process appears to be attractive for the large-scale production of this thermostable enzyme useful for the manufacture of inverted sugar syrup.

  10. Crystal structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Meiying; Cooper, David; Grossoehmerb, Nickolas; Yu, Minmin; Hung, Li-Wei; Cieslik, Murcin; Derewendaro, Urszula; Lesley, Scott; Wilson, Ian; Giedrocb, David; Derewenda, Zygmunt

    2009-06-06

    The GntR superfamily of dimeric transcription factors, with more than 6200 members encoded in bacterial genomes, are characterized by N-terminal winged helix (WH) DNA-binding domains and diverse C-terminal, regulatory domains, which provide a basis for the classification of the constituent families. The largest of these families, FadR, contains nearly 3000 proteins with all a-helical regulatory domains classified into two related Pfam families: FadR{_}C and FCD. Only two crystal structures of the FadR family members, i.e. the E. coli FadR protein and the LldR from C. glutamicum, have been described to date in literature. Here we describe the crystal structure of TM0439, a GntR regulator with an FCD domain, found in the Thermotoga maritima genome. The FCD domain is similar to that of the LldR regulator, and contains a buried metal binding site. Using atomic absorption spectroscopy and Trp fluorescence, we show that the recombinant protein contains bound Ni{sup 2+} ions, but it is able to bind Zn{sup 2+} with K{sub D} < 70 nM . We conclude that Zn{sup 2+} is the likely physiological metal, where it may perform either or both structural and regulatory roles. Finally, we compare the TM0439 structure to two other FadR family structures recently deposited by Structural Genomics consortia. The results call for a revision in the classification of the FadR family of transcription factors.

  11. Structural and biochemical characterization of the β-N-acetylglucosaminidase from Thermotoga maritima: toward rationalization of mechanistic knowledge in the GH73 family.

    Science.gov (United States)

    Lipski, Alexandra; Hervé, Mireille; Lombard, Vincent; Nurizzo, Didier; Mengin-Lecreulx, Dominique; Bourne, Yves; Vincent, Florence

    2015-03-01

    Members of the GH73 glycosidase family cleave the β-1,4-glycosidic bond between the N-acetylglucosaminyl (GlcNAc) and N-acetylmuramyl (MurNAc) moieties in bacterial peptidoglycan. A catalytic mechanism has been proposed for members FlgJ, Auto, AcmA and Atl(WM) and the structural analysis of FlgJ and Auto revealed a conserved α/β fold reminiscent of the distantly related GH23 lysozyme. Comparison of the active site residues reveals variability in the nature of the catalytic general base suggesting two distinct catalytic mechanisms: an inverting mechanism involving two distant glutamate residues and a substrate-assisted mechanism involving anchimeric assistance by the C2-acetamido group of the GlcNAc moiety. Herein, we present the biochemical characterization and crystal structure of TM0633 from the hyperthermophilic bacterium Thermotoga maritima. TM0633 adopts the α/β fold of the family and displays β-N-acetylglucosaminidase activity on intact peptidoglycan sacculi. Site-directed mutagenesis identifies Glu34, Glu65 and Tyr118 as important residues for catalysis. A thorough bioinformatic analysis of the GH73 sequences identified five phylogenetic clusters. TM0633, FlgJ and Auto belong to a group of three clusters that conserve two carboxylate residues involved in a classical inverting acid-base mechanism. Members of the other two clusters lack a conserved catalytic general base supporting a substrate-assisted mechanism. Molecular modeling of representative members from each cluster suggests that variability in length of the β-hairpin region above the active site confers ligand-binding specificity and modulates the catalytic mechanisms within the GH73 family. © The Author 2014. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  12. Biohydrogen production from hyperthermophilic anaerobic digestion of fruit and vegetable wastes in seawater: Simplification of the culture medium of Thermotoga maritima.

    Science.gov (United States)

    Saidi, Rafika; Liebgott, Pierre Pol; Gannoun, Hana; Ben Gaida, Lamia; Miladi, Baligh; Hamdi, Moktar; Bouallagui, Hassib; Auria, Richard

    2018-01-01

    Biohydrogen production by the hyperthermophilic and halophilic bacterium T. maritima, using fruit and vegetable wastes as the carbon and energy sources was studied. Batch fermentation cultures showed that the use of a culture medium containing natural seawater and fruit and vegetable wastes can replace certain components (CaCl 2 , MgCl 2 , Balch's oligo-elements, yeast extract, KH 2 PO 4 and K 2 HPO 4 ) present in basal medium. However, a source of nitrogen and sulfur remained necessary for biohydrogen production. When fruit and vegetable waste collected from a wholesale market landfill was used, no decreases in total H 2 production (139 mmol L -1 ) or H 2 yield (3.46 mol mol -1 ) was observed. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Sugar Transport and Metabolism in Thermotoga

    Energy Technology Data Exchange (ETDEWEB)

    Noll, Kenneth M.; Romano, Antonio H.

    2003-02-11

    The work conducted under this grant demonstrated that the hyperthermophilic bacterium Thermotoga neapolitana carries out glucose and lactose transport in a sodium-dependent manner and that energization of anaerobic cells is required to observe transport. We also demonstrated that Thermotoga maritima carries out maltose and glucose transport using periplasmic sugar binding proteins. We began defining patterns of expression of genes encoding sugar transport and catabolic functions in both T. maritima and T. neapolitana. We began a collaborative effort to identify all the genes regulated at the transcriptional level in response to sugars substrates. These funds also allowed us to begin an examination of the functions of several periplasmic substrate binding proteins encoded in the genome of T. maritima.

  14. Construction and transformation of a Thermotoga-E. coli shuttle vector.

    Science.gov (United States)

    Han, Dongmei; Norris, Stephen M; Xu, Zhaohui

    2012-01-06

    Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems for understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp. Methods for preparing and handling Thermotoga solid cultures under aerobic conditions were optimized. A plating efficiency of ~50% was achieved when the bacterial cells were embedded in 0.3% Gelrite. A Thermotoga-E. coli shuttle vector pDH10 was constructed using pRQ7, a cryptic mini-plasmid found in T. sp. RQ7. Plasmid pDH10 was introduced to T. maritima and T. sp. RQ7 by electroporation and liposome-mediated transformation. Transformants were isolated, and the transformed kanamycin resistance gene (kan) was detected from the plasmid DNA extracts of the recombinant strains by PCR and was confirmed by restriction digestions. The transformed DNA was stably maintained in both Thermotoga and E. coli even without the selective pressure. Thermotoga are transformable by multiple means. Recombinant Thermotoga strains have been isolated for the first time. A heterologous kan gene is functionally expressed and stably maintained in Thermotoga.

  15. Construction and transformation of a Thermotoga-E. coli shuttle vector

    Directory of Open Access Journals (Sweden)

    Han Dongmei

    2012-01-01

    Full Text Available Abstract Background Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems for understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp. Results Methods for preparing and handling Thermotoga solid cultures under aerobic conditions were optimized. A plating efficiency of ~50% was achieved when the bacterial cells were embedded in 0.3% Gelrite. A Thermotoga-E. coli shuttle vector pDH10 was constructed using pRQ7, a cryptic mini-plasmid found in T. sp. RQ7. Plasmid pDH10 was introduced to T. maritima and T. sp. RQ7 by electroporation and liposome-mediated transformation. Transformants were isolated, and the transformed kanamycin resistance gene (kan was detected from the plasmid DNA extracts of the recombinant strains by PCR and was confirmed by restriction digestions. The transformed DNA was stably maintained in both Thermotoga and E. coli even without the selective pressure. Conclusions Thermotoga are transformable by multiple means. Recombinant Thermotoga strains have been isolated for the first time. A heterologous kan gene is functionally expressed and stably maintained in Thermotoga.

  16. Genome Sequence of Thermotoga sp Strain RQ2, a Hyperthermophilic Bacterium Isolated from a Geothermally Heated Region of the Seafloor near Ribeira Quente, the Azores

    Energy Technology Data Exchange (ETDEWEB)

    Swithers, Kristen S [University of Connecticut, Storrs; DiPippo, Jonathan L [University of Connecticut, Storrs; Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Lykidis, A [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Stetter, Karl O [Universitat Regensburg, Regensburg, Germany; Nelson, Karen E [J. Craig Venter Institute; Gogarten, Peter [University of Connecticut, Storrs; Noll, Kenneth M [University of Connecticut, Storrs

    2011-01-01

    Thermotoga sp. strain RQ2 is probably a strain of Thermotoga maritima. Its complete genome sequence allows for an examination of the extent and consequences of gene flow within Thermotoga species and strains. Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabolism. Its genome also encodes an apparent fructose phosphotransferase system (PTS) sugar transporter. This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Thermotogales. These are the first reported PTS transporters in the Thermotogales.

  17. Over-expression of xylanolytic α-glucuronidase from Thermotoga ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-07-18

    Jul 18, 2008 ... The GH67α-glucuronidase encoded by aguA of Thermotoga maritima is one of the most thermostable α- glucuronidases described to date and thus has considerable potential in industrial application. The enzyme was higher expressed by using the novel pHsh expression vector than in pET vector in.

  18. Reduction of Folate by Dihydrofolate Reductase from Thermotoga maritima

    NARCIS (Netherlands)

    Loveridge, E Joel; Hroch, Lukas; Hughes, Robert L; Williams, Thomas; Davies, Rhidian L; Angelastro, Antonio; Luk, Louis Y P; Maglia, Giovanni; Allemann, Rudolf K

    2017-01-01

    Mammalian dihydrofolate reductases (DHFR) catalyse the reduction of folate more efficiently than the equivalent bacterial enzymes, despite typically having similar efficiencies for the reduction of their natural substrate dihydrofolate. In contrast, we show here that DHFR from the hyperthermophilic

  19. Thermotoga subterranea sp. nov., a new thermophilic bacterium isolated from a continental oil reservoir.

    Science.gov (United States)

    Jeanthon, C; Reysenbach, A L; L'Haridon, S; Gambacorta, A; Pace, N R; Glénat, P; Prieur, D

    1995-08-01

    A thermophilic, strictly anaerobic bacterium, designated strain SL1, was isolated from a deep, continental oil reservoir in the East Paris Basin (France). This organism grew between 50 and 75 degrees C, with an optimum at 70 degrees C. It was inhibited by elemental sulfur and was able to reduce cystine and thiosulfate to hydrogen sulfide. The G+C content (40 mol%), the presence of a lipid structure unique to the genus Thermotoga, and the 16S rRNA sequence of strain SL1 indicated that the isolate belongs to the genus Thermotoga. Based on DNA-DNA hybridization, isolate SL1 does not show species-level similarity with the recognized species T. maritima, T. neapolitana, and T. thermarum. Based on this description of strain SL1, we propose the recognition of a new species: Thermotoga subterranea.

  20. Backbone assignments for the SPOUT methyltransferase MTT Tm , a knotted protein from Thermotoga maritima.

    Science.gov (United States)

    Burban, David J; Jennings, Patricia A

    2017-10-01

    The SPOUT family of methyltransferase proteins is noted for containing a deep trefoil knot in their defining backbone fold. This unique fold is of high interest for furthering the understanding of knots in proteins. Here, we report the 1 H, 13 C, 15 N assignments for MTT Tm , a canonical member of the SPOUT family. This protein is unique, as it is one of the smallest members of the family, making it an ideal system for probing the unique properties of the knot. Our present work represents the foundation for further studies into the topology of MTT Tm , and understanding how its structure affects both its folding and function.

  1. Crystal Structure of the Flagellar Rotor Protein FliN from Thermotoga maritima†

    Science.gov (United States)

    Brown, Perry N.; Mathews, Michael A. A.; Joss, Lisa A.; Hill, Christopher P.; Blair, David F.

    2005-01-01

    FliN is a component of the bacterial flagellum that is present at levels of more than 100 copies and forms the bulk of the C ring, a drum-shaped structure at the inner end of the basal body. FliN interacts with FliG and FliM to form the rotor-mounted switch complex that controls clockwise-counterclockwise switching of the motor. In addition to its functions in motor rotation and switching, FliN is thought to have a role in the export of proteins that form the exterior structures of the flagellum (the rod, hook, and filament). Here, we describe the crystal structure of most of the FliN protein of Thermotoga maritima. FliN is a tightly intertwined dimer composed mostly of β sheet. Several well-conserved hydrophobic residues form a nonpolar patch on the surface of the molecule. A mutation in the hydrophobic patch affected both flagellar assembly and switching, showing that this surface feature is important for FliN function. The association state of FliN in solution was studied by analytical ultracentrifugation, which provided clues to the higher-level organization of the protein. T. maritima FliN is primarily a dimer in solution, and T. maritima FliN and FliM together form a stable FliM1-FliN4 complex. Escherichia coli FliN forms a stable tetramer in solution. The arrangement of FliN subunits in the tetramer was modeled by reference to the crystal structure of tetrameric HrcQBC, a related protein that functions in virulence factor secretion in Pseudomonas syringae. The modeled tetramer is elongated, with approximate dimensions of 110 by 40 by 35Å, and it has a large hydrophobic cleft formed from the hydrophobic patches on the dimers. On the basis of the present data and available electron microscopic images, we propose a model for the organization of FliN subunits in the C ring. PMID:15805535

  2. Natural transformation of Thermotoga sp. strain RQ7.

    Science.gov (United States)

    Han, Dongmei; Xu, Hui; Puranik, Rutika; Xu, Zhaohui

    2014-05-10

    Thermotoga species are organisms of enormous interest from a biotechnological as well as evolutionary point of view. Genetic modifications of Thermotoga spp. are often desired in order to fully release their multifarious potentials. Effective transformation of recombinant DNA into these bacteria constitutes a critical step of such efforts. This study aims to establish natural competency in Thermotoga spp. and to provide a convenient method to transform these organisms. Foreign DNA was found to be relatively stable in the supernatant of a Thermotoga culture for up to 6 hours. Adding donor DNA to T. sp. strain RQ7 at its early exponential growth phase (OD600 0.18 ~ 0.20) resulted in direct acquisition of the DNA by the cells. Both T. neapolitana chromosomal DNA and Thermotoga-E. coli shuttle vectors effectively transformed T. sp. strain RQ7, rendering the cells resistance to kanamycin. The kan gene carried by the shuttle vector pDH10 was detected by PCR from the plasmid extract of the transformants, and the amplicons were verified by restriction digestions. A procedure for natural transformation of Thermotoga spp. was established and optimized. With the optimized method, T. sp. strain RQ7 sustained a transformation frequency in the order of 10⁻⁷ with both genomic and plasmid DNA. T. sp. strain RQ7 cells are naturally transformable during their early exponential phase. They acquire DNA from both closely and distantly related species. Both chromosomal DNA and plasmid DNA serve as suitable substrates for transformation. Our findings lend a convenient technical tool for the genetic engineering of Thermotoga spp.

  3. Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme.

    Science.gov (United States)

    Kim, Byoung-Chan; Lee, Yoon-Hee; Lee, Han-Seung; Lee, Dong-Woo; Choe, Eun-Ah; Pyun, Yu-Ryang

    2002-06-18

    Gene araA encoding an L-arabinose isomerase (AraA) from the hyperthermophile, Thermotoga neapolitana 5068 was cloned, sequenced, and expressed in Escherichia coli. The gene encoded a polypeptide of 496 residues with a calculated molecular mass of 56677 Da. The deduced amino acid sequence has 94.8% identical amino acids compared with the residues in a putative L-arabinose isomerase of Thermotoga maritima. The recombinant enzyme expressed in E. coli was purified to homogeneity by heat treatment, ion exchange chromatography and gel filtration. The thermophilic enzyme had a maximum activity of L-arabinose isomerization and D-galactose isomerization at 85 degrees C, and required divalent cations such as Co(2+) and Mn(2+) for its activity and thermostability. The apparent K(m) values of the enzyme for L-arabinose and D-galactose were 116 mM (v(max), 119 micromol min(-1) mg(-1)) and 250 mM (v(max), 14.3 micromol min(-1) mg(-1)), respectively, that were determined in the presence of both 1 mM Co(2+) and 1 mM Mn(2+). A 68% conversion of D-galactose to D-tagatose was obtained using the recombinant enzyme at the isomerization temperature of 80 degrees C.

  4. Production, characterization, and assessment of a stable analog of the response regulator CheY-phosphate from Thermotoga maritima.

    Science.gov (United States)

    Beyersdorf, Matthew S; Sircar, Ria; Lookadoo, Daniel B; Bottone, Cory J; Lynch, Michael J; Crane, Brian R; Halkides, Christopher J

    2017-08-01

    Phosphorylation of CheY promotes association with the flagellar motor and ultimately controls the directional bias of the motor. However, biochemical studies of activated CheY-phosphate have been challenging due to the rapid hydrolysis of the aspartyl-phosphate in vitro. An inert analog of Tm CheY-phosphate, phosphono-CheY, was synthesized by chemical modification and purified by cation-exchange chromatography. Changes in HPLC retention times, chemical assays for phosphate and free thiol, and mass spectrometry experiments demonstrate modification of Cys54 with a phosphonomethyl group. Additionally, a crystal structure showed electron density for the phosphonomethyl group at Cys54, consistent with a modification at that position. Subsequent biochemical experiments confirmed that protein crystals were phosphono-CheY. Isothermal titration calorimetry and fluorescence polarization binding assays demonstrated that phosphono-CheY bound a peptide derived from FliM, a native partner of CheY-phosphate, with a dissociation constant of ∼29 µM, at least sixfold more tightly than unmodified CheY. Taken together these results suggest that Tm phosphono-CheY is a useful and unique analog of Tm CheY-phosphate. © 2017 The Protein Society.

  5. Hydrogen production from carrot pulp by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    NARCIS (Netherlands)

    Vrije, de G.J.; Budde, M.A.W.; Lips, S.J.J.; Bakker, R.R.; Mars, A.E.; Claassen, P.A.M.

    2010-01-01

    Hydrogen was produced from carrot pulp hydrolysate, untreated carrot pulp and (mixtures of) glucose and fructose by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana in pH-controlled bioreactors. Carrot pulp hydrolysate was obtained after enzymatic hydrolysis

  6. Hydrogen Production by the Thermophilic Bacterium Thermotoga neapolitana

    Science.gov (United States)

    Pradhan, Nirakar; Dipasquale, Laura; d’Ippolito, Giuliana; Panico, Antonio; Lens, Piet N. L.; Esposito, Giovanni; Fontana, Angelo

    2015-01-01

    As the only fuel that is not chemically bound to carbon, hydrogen has gained interest as an energy carrier to face the current environmental issues of greenhouse gas emissions and to substitute the depleting non-renewable reserves. In the last years, there has been a significant increase in the number of publications about the bacterium Thermotoga neapolitana that is responsible for production yields of H2 that are among the highest achievements reported in the literature. Here we present an extensive overview of the most recent studies on this hyperthermophilic bacterium together with a critical discussion of the potential of fermentative production by this bacterium. The review article is organized into sections focused on biochemical, microbiological and technical issues, including the effect of substrate, reactor type, gas sparging, temperature, pH, hydraulic retention time and organic loading parameters on rate and yield of gas production. PMID:26053393

  7. Hydrogen Production by the Thermophilic Bacterium Thermotoga neapolitana

    Directory of Open Access Journals (Sweden)

    Nirakar Pradhan

    2015-06-01

    Full Text Available As the only fuel that is not chemically bound to carbon, hydrogen has gained interest as an energy carrier to face the current environmental issues of greenhouse gas emissions and to substitute the depleting non-renewable reserves. In the last years, there has been a significant increase in the number of publications about the bacterium Thermotoga neapolitana that is responsible for production yields of H2 that are among the highest achievements reported in the literature. Here we present an extensive overview of the most recent studies on this hyperthermophilic bacterium together with a critical discussion of the potential of fermentative production by this bacterium. The review article is organized into sections focused on biochemical, microbiological and technical issues, including the effect of substrate, reactor type, gas sparging, temperature, pH, hydraulic retention time and organic loading parameters on rate and yield of gas production.

  8. Phytochemical study, antioxidant and antibacterial activities of Stemodia maritima

    Directory of Open Access Journals (Sweden)

    Francisca R. L. da Silva

    2014-01-01

    Full Text Available Stemodinol, a new natural compound, together with known compounds including jaceidin, stemodin, stemodinoside B, isocrenatoside, verbascoside, crenatoside, and isoverbascoside, were isolated from Stemodia maritima Linn. The antioxidant (DPPH method and antimicrobial activities of stemodin, stemodinoside B, and crenatoside were investigated. Among the components tested, only crenatoside isolated from the roots showed a high antioxidant power. Stemodin and stemodinoside B exhibited antibacterial activities.

  9. Genome sequence of the Thermotoga thermarum type strain (LA3(T)) from an African solfataric spring.

    Science.gov (United States)

    Göker, Markus; Spring, Stefan; Scheuner, Carmen; Anderson, Iain; Zeytun, Ahmet; Nolan, Matt; Lucas, Susan; Tice, Hope; Del Rio, Tijana Glavina; Cheng, Jan-Fang; Han, Cliff; Tapia, Roxanne; Goodwin, Lynne A; Pitluck, Sam; Liolios, Konstantinos; Mavromatis, Konstantinos; Pagani, Ioanna; Ivanova, Natalia; Mikhailova, Natalia; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Land, Miriam; Hauser, Loren; Chang, Yun-Juan; Jeffries, Cynthia D; Rohde, Manfred; Detter, John C; Woyke, Tanja; Bristow, James; Eisen, Jonathan A; Markowitz, Victor; Hugenholtz, Philip; Kyrpides, Nikos C; Klenk, Hans-Peter; Lapidus, Alla

    2014-06-15

    Thermotoga thermarum Windberger et al. 1989 is a member to the genomically well characterized genus Thermotoga in the phylum 'Thermotogae'. T. thermarum is of interest for its origin from a continental solfataric spring vs. predominantly marine oil reservoirs of other members of the genus. The genome of strain LA3T also provides fresh data for the phylogenomic positioning of the (hyper-)thermophilic bacteria. T. thermarum strain LA3(T) is the fourth sequenced genome of a type strain from the genus Thermotoga, and the sixth in the family Thermotogaceae to be formally described in a publication. Phylogenetic analyses do not reveal significant discrepancies between the current classification of the group, 16S rRNA gene data and whole-genome sequences. Nevertheless, T. thermarum significantly differs from other Thermotoga species regarding its iron-sulfur cluster synthesis, as it contains only a minimal set of the necessary proteins. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,039,943 bp long chromosome with its 2,015 protein-coding and 51 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  10. Development of microsatellite markers for Suriana maritima (Surianaceae) using next-generation sequencing technology.

    Science.gov (United States)

    Chen, W S; Zhao, G; Jian, S G; Wang, Z F

    2015-10-30

    Our objective was to develop microsatellite markers for use in assessing genetic variation in the small shrub or tree species Suriana maritima (Surianaceae). In China, this species is found only as a few fragmented populations and individuals on the Paracel Islands. Using next-generation genome sequencing methodology, we developed 17 novel microsatellite markers for S. maritima. Fifty-four individuals from six populations of S. maritima were examined for polymorphisms; only one allele was detected for each of the markers. Microsatellite loci developed indicate a complete absence of genetic diversity for S. maritima on the Paracel Islands in China. These markers will be useful for examining genetic variation among S. maritima populations in other areas of the world.

  11. Fluid dynamics of two-dimensional pollination in Ruppia maritima

    Science.gov (United States)

    Musunuri, Naga; Bunker, Daniel; Pell, Susan; Pell, Fischer; Singh, Pushpendra

    2016-11-01

    The aim of this work is to understand the physics underlying the mechanisms of two-dimensional aquatic pollen dispersal, known as hydrophily. We observed two mechanisms by which the pollen released from male inflorescences of Ruppia maritima is adsorbed on a water surface: (i) inflorescences rise above the surface and after they mature their pollen mass falls onto the surface as clumps and disperses on the surface; (ii) inflorescences remain below the surface and produce air bubbles which carry their pollen mass to the surface where it disperses. In both cases dispersed pollen masses combined under the action of capillary forces to form pollen rafts. This increases the probability of pollination since the capillary force on a pollen raft towards a stigma is much larger than on a single pollen grain. The presence of a trace amount of surfactant can disrupt the pollination process so that the pollen is not transported or captured on the water surface. National Science Foundation.

  12. Diterpene and other constituents from Stemodia maritima (Scrophulariaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Francisco E.A.; Oliveira, Maria da Conceicao F. de; Vasconcelos, Jackson N.; Mafezoli, Jair; Arriaga, Angela M.C., E-mail: angelamcarriaga@yahoo.com.b [Universidade Federal do Ceara (DQOI/UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica. Curso de Pos-Graducao em Quimica; Lima, Jefferson Q. [Instituto Federal do Ceara, Juazeiro do Norte, CE (Brazil). Curso de Engenharia Ambiental; Santiago, Gilvandete M.P. [Universidade Federal do Ceara (DQOI/UFC), Fortaleza, CE (Brazil). Dept. de Farmacia; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense Darcy Ribeiro (CCT/UENF), Campos dos Goytacazes, RJ (Brazil). Centro de Ciencias e Tecnologias

    2010-07-01

    A new diterpene, (5S{sup *},8S{sup *},9R{sup *},10S{sup *})-11{beta},12{beta}-epoxy-9{alpha}-hydroxy-19(4 -> 3)abeo-abieta-3,13-diene-19,18-olide, together with the known compounds stemodin, D-mannitol, betulinic acid, a mixture of 3{beta}-O-{beta}-D-glucopyranosyl-{beta}-sitosterol and 3{beta}-O-{beta}-D-glucopyranosylstigmasterol and 5,7,4'-trihydroxy-3,8,3'-trimethoxyflavone were isolated from the leaves and stems of Stemodia maritima. Structural elucidation of all compounds was based on interpretation of spectral data, mainly NMR (1D and 2D) and MS, including comparison with values described in the literature. (author)

  13. Diterpene and other constituents from Stemodia maritima (Scrophulariaceae)

    International Nuclear Information System (INIS)

    Rodrigues, Francisco E.A.; Oliveira, Maria da Conceicao F. de; Vasconcelos, Jackson N.; Mafezoli, Jair; Arriaga, Angela M.C.; Lima, Jefferson Q.; Santiago, Gilvandete M.P.; Braz-Filho, Raimundo

    2010-01-01

    A new diterpene, (5S * ,8S * ,9R * ,10S * )-11β,12β-epoxy-9α-hydroxy-19(4 -> 3)abeo-abieta-3,13-diene-19,18-olide, together with the known compounds stemodin, D-mannitol, betulinic acid, a mixture of 3β-O-β-D-glucopyranosyl-β-sitosterol and 3β-O-β-D-glucopyranosylstigmasterol and 5,7,4'-trihydroxy-3,8,3'-trimethoxyflavone were isolated from the leaves and stems of Stemodia maritima. Structural elucidation of all compounds was based on interpretation of spectral data, mainly NMR (1D and 2D) and MS, including comparison with values described in the literature. (author)

  14. Insights into thermoadaptation and the evolution of mesophily from the bacterial phylum Thermotogae.

    Science.gov (United States)

    Pollo, Stephen M J; Zhaxybayeva, Olga; Nesbø, Camilla L

    2015-09-01

    Thermophiles are extremophiles that grow optimally at temperatures >45 °C. To survive and maintain function of their biological molecules, they have a suite of characteristics not found in organisms that grow at moderate temperature (mesophiles). At the cellular level, thermophiles have mechanisms for maintaining their membranes, nucleic acids, and other cellular structures. At the protein level, each of their proteins remains stable and retains activity at temperatures that would denature their mesophilic homologs. Conversely, cellular structures and proteins from thermophiles may not function optimally at moderate temperatures. These differences between thermophiles and mesophiles presumably present a barrier for evolutionary transitioning between the 2 lifestyles. Therefore, studying closely related thermophiles and mesophiles can help us determine how such lifestyle transitions may happen. The bacterial phylum Thermotogae contains hyperthermophiles, thermophiles, mesophiles, and organisms with temperature ranges wide enough to span both thermophilic and mesophilic temperatures. Genomic, proteomic, and physiological differences noted between other bacterial thermophiles and mesophiles are evident within the Thermotogae. We argue that the Thermotogae is an ideal group of organisms for understanding of the response to fluctuating temperature and of long-term evolutionary adaptation to a different growth temperature range.

  15. Nickel tolerance, accumulation and subcellular distribution in the halophytes Sesuvium portulacastrum and Cakile maritima.

    Science.gov (United States)

    Fourati, Emna; Wali, Mariem; Vogel-Mikuš, Katarina; Abdelly, Chedly; Ghnaya, Tahar

    2016-11-01

    It has been shown that halophytes are able to successfully cope with heavy metal toxicity, suggesting their possible use for remediation of metal contaminated soils. In this work, Ni tolerance and accumulation in two halophytes, Sesuvium portulacastrum (L.) L. and Cakile maritima Scop. was investigated. Seedlings of both species were subjected hydroponically during 21 days to 0, 25, 50, and 100 μM of NiCl 2 . The growth and photosynthesis parameters revealed that S. portulacastrum tolerates Ni better than C. maritima. The photosynthesis activity, chlorophyll content and photosystem II integrity were less impacted in Ni-treated S. portulacastrum as compared to C. maritima, although, Ni accumulated in higher concentrations in the shoots of S. portulacastrum (1050 μg g -1 DW) than in those of C. maritima (550 μg g -1 DW). The subcellular fractionation of Ni in the shoots of both species showed that C. maritima accumulated about 65% of Ni in the soluble fraction, while 28% was associated with the cell walls. In S. portulacastrum 44% of the total cellular Ni was seen in the soluble fraction and 43% was bound to the cell walls. It can be concluded that S. portulacastrum tolerates Ni better than C. maritima, most probably due to a better ability to sequester Ni in the cell walls, restricting its accumulation in the soluble fraction. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  16. Metabolic and physiological adjustment of Suaeda maritima to combined salinity and hypoxia.

    Science.gov (United States)

    Behr, Jan H; Bouchereau, Alain; Berardocco, Solenne; Seal, Charlotte E; Flowers, Timothy J; Zörb, Christian

    2017-04-01

    Suaeda maritima is a halophyte commonly found on coastal wetlands in the intertidal zone. Due to its habitat S. maritima has evolved tolerance to high salt concentrations and hypoxic conditions in the soil caused by periodic flooding. In the present work, the adaptive mechanisms of S. maritima to salinity combined with hypoxia were investigated on a physiological and metabolic level. To compare the adaptive mechanisms to deficient, optimal and stressful salt concentrations, S. maritima plants were grown in a hydroponic culture under low, medium and high salt concentrations. Additionally, hypoxic conditions were applied to investigate the impact of hypoxia combined with different salt concentrations. A non-targeted metabolic approach was used to clarify the biochemical pathways underlying the metabolic and physiological adaptation mechanisms of S. maritima . Roots exposed to hypoxic conditions showed an increased level of tricarboxylic acid (TCA)-cycle intermediates such as succinate, malate and citrate. During hypoxia, the concentration of free amino acids increased in shoots and roots. Osmoprotectants such as proline and glycine betaine increased in concentrations as the external salinity was increased under hypoxic conditions. The combination of high salinity and hypoxia caused an ionic imbalance and an increase of metabolites associated with osmotic stress and photorespiration, indicating a severe physiological and metabolic response under these conditions. Disturbed proline degradation in the roots induced an enhanced proline accumulation under hypoxia. The enhanced alanine fermentation combined with a partial flux of the TCA cycle might contribute to the tolerance of S. maritima to hypoxic conditions. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  17. Reconstructed ancestral Myo-inositol-3-phosphate synthases indicate that ancestors of the Thermococcales and Thermotoga species were more thermophilic than their descendants.

    Directory of Open Access Journals (Sweden)

    Nicholas C Butzin

    Full Text Available The bacterial genomes of Thermotoga species show evidence of significant interdomain horizontal gene transfer from the Archaea. Members of this genus acquired many genes from the Thermococcales, which grow at higher temperatures than Thermotoga species. In order to study the functional history of an interdomain horizontally acquired gene we used ancestral sequence reconstruction to examine the thermal characteristics of reconstructed ancestral proteins of the Thermotoga lineage and its archaeal donors. Several ancestral sequence reconstruction methods were used to determine the possible sequences of the ancestral Thermotoga and Archaea myo-inositol-3-phosphate synthase (MIPS. These sequences were predicted to be more thermostable than the extant proteins using an established sequence composition method. We verified these computational predictions by measuring the activities and thermostabilities of purified proteins from the Thermotoga and the Thermococcales species, and eight ancestral reconstructed proteins. We found that the ancestral proteins from both the archaeal donor and the Thermotoga most recent common ancestor recipient were more thermostable than their descendants. We show that there is a correlation between the thermostability of MIPS protein and the optimal growth temperature (OGT of its host, which suggests that the OGT of the ancestors of these species of Archaea and the Thermotoga grew at higher OGTs than their descendants.

  18. Reconstructed ancestral Myo-inositol-3-phosphate synthases indicate that ancestors of the Thermococcales and Thermotoga species were more thermophilic than their descendants.

    Science.gov (United States)

    Butzin, Nicholas C; Lapierre, Pascal; Green, Anna G; Swithers, Kristen S; Gogarten, J Peter; Noll, Kenneth M

    2013-01-01

    The bacterial genomes of Thermotoga species show evidence of significant interdomain horizontal gene transfer from the Archaea. Members of this genus acquired many genes from the Thermococcales, which grow at higher temperatures than Thermotoga species. In order to study the functional history of an interdomain horizontally acquired gene we used ancestral sequence reconstruction to examine the thermal characteristics of reconstructed ancestral proteins of the Thermotoga lineage and its archaeal donors. Several ancestral sequence reconstruction methods were used to determine the possible sequences of the ancestral Thermotoga and Archaea myo-inositol-3-phosphate synthase (MIPS). These sequences were predicted to be more thermostable than the extant proteins using an established sequence composition method. We verified these computational predictions by measuring the activities and thermostabilities of purified proteins from the Thermotoga and the Thermococcales species, and eight ancestral reconstructed proteins. We found that the ancestral proteins from both the archaeal donor and the Thermotoga most recent common ancestor recipient were more thermostable than their descendants. We show that there is a correlation between the thermostability of MIPS protein and the optimal growth temperature (OGT) of its host, which suggests that the OGT of the ancestors of these species of Archaea and the Thermotoga grew at higher OGTs than their descendants.

  19. NaCl salinity affects lateral root development in Plantago maritima

    NARCIS (Netherlands)

    Rubinigg, M; Wenisch, J; Elzenga, JTM; Stulen, [No Value

    2004-01-01

    Root growth and morphology were assessed weekly in hydroponically-grown seedlings of the halophyte Plantago maritima L. during exposure to 0, 50, 100 and 200 mM NaCl for 21 d. Relative growth rate was reduced by 25% at 200 mM NaCl. The lower NaCl treatments did not affect relative growth rates.

  20. Dendrochronology of Atriplex portulacoides and Artemisia maritima in Wadden Sea salt marshes

    NARCIS (Netherlands)

    Decuyper, M.; Slim, P.A.; Loon-Steensma, van J.M.

    2014-01-01

    The study uses a rather unusual method, dendrochronology, to investigate the growth and survival of Atriplex portulacoides L. and Artemisia maritima L. on salt marshes at two field sites on the Dutch North Sea barrier islands of Terschelling and Ameland. By providing information on longevity of

  1. Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte).

    Science.gov (United States)

    Ellouzi, Hasna; Hamed, Karim Ben; Cela, Jana; Munné-Bosch, Sergi; Abdelly, Chedly

    2011-06-01

    Early changes in physiological and oxidative status induced by salt stress were monitored in two Brassicaceae plants differing in their tolerance to salinity, Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Growth response and antioxidant defense of C. maritima under 400 mM NaCl were compared with those of A. thaliana exposed to 100 mM NaCl. Salinity induced early growth reduction that is less pronounced in C. maritima than in A. thaliana. Maximum hydrogen peroxide (H₂O₂) level occurred in the leaves of both species 4 h after the onset of salt treatment. A rapid decline in H₂O₂ concentration was observed thereafter in C. maritima, whereas it remained high in A. thaliana. Correlatively, superoxide dismutase, catalase and peroxidase activities increased at 4 h of treatment in C. maritima and decreased thereafter. However, the activity of these enzymes remained higher in treated plants than that in controls, regardless of the duration of treatment, in A. thaliana. The concentrations of malondialdehyde (MDA) reached maximum values at 24 h of salt stress in both species. Again, MDA levels decreased later in C. maritima, but remained high in A. thaliana. The contents of α-tocopherol remained constant during salt stress in C. maritima and decreased during the first 24 h of salt stress and then remained low in A. thaliana. The results clearly showed that C. maritima, in contrast to A. thaliana, can rapidly evolve physiological and antioxidant mechanisms to adapt to salt and manage the oxidative stress. This may explain, at least partially, the difference in salt tolerance between halophytes and glycophytes. Copyright © Physiologia Plantarum 2011.

  2. Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

    DEFF Research Database (Denmark)

    Jovanovic, Zeljko; Pedersen, Mia Østergaard; Larsen, Morten

    2015-01-01

    Zostera marina and Ruppia maritima often share the same habitat, but R. maritima appears more resistant to environmental stress. We investigated the impact of light intensity and water column O2 concentrations on radial oxygen loss (ROL), in young specimens of Z. marina and R. maritima. Planar......, respectively. These values declined by 71 and 60% in darkness. However, both species were able to maintain ROL as long as ambient O2 levels remained >50% air saturation. The calculated ROL integrated over a 24 h cycle was 48.8 ± 10.6 nmol O2 plant−1 d−1 (n = 3) for R. maritima and 30% less for Z. marina...

  3. Complete genome sequence of Hippea maritima type strain (MH2T)

    Energy Technology Data Exchange (ETDEWEB)

    Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Lu, Megan [Los Alamos National Laboratory (LANL); Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute

    2011-01-01

    Hippea maritima (Miroshnichenko et al. 1999) is the type species of the genus Hippea, which belongs to the family Desulfurellaceae within the class Deltaproteobacteria. The anaerobic, moderately thermophilic marine sulfur-reducer was first isolated from shallow-water hot vents in Matipur Harbor, Papua New Guinea. H. maritima was of interest for genome se- quencing because of its isolated phylogenetic location, as a distant next neighbor of the ge- nus Desulfurella. Strain MH2T is the first type strain from the order Desulfurellales with a com- pletely sequenced genome. The 1,694,430 bp long linear genome with its 1,723 protein- coding and 57 RNA genes consists of one circular chromosome and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  4. XX/XY system of sex determination in the geophilomorph centipede Strigamia maritima

    Czech Academy of Sciences Publication Activity Database

    Green, J. E.; Dalíková, Martina; Sahara, K.; Marec, František; Akam, M.

    2016-01-01

    Roč. 11, č. 2 (2016), č. článku e0150292. E-ISSN 1932-6203 R&D Projects: GA AV ČR IAA600960925; GA ČR(CZ) GA14-22765S Institutional support: RVO:60077344 Keywords : sex determination * Strigamia maritima * XX/XY system Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.806, year: 2016 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150292

  5. The aquatic vascular plant Ruppia maritima as an indicator organism for contaminated sediments

    Energy Technology Data Exchange (ETDEWEB)

    Tagliabue, M.D.; Thursby, G.B.; Walker, H.A. [Environmental Protection Agency, Narragansett, RI (United States); Johnston, R.K.

    1995-12-31

    An ongoing estuarine ecological risk assessment case study for the Portsmouth Naval Shipyard in the Great Bay Estuary (New Hampshire, Maine) was the catalyst to continue development a rooted aquatic plant sediment toxicity test. Laboratory studies were conducted to evaluate effects of lead, the primary site contaminant on R. maritima in the Great Bay. Although the aquatic vascular plant Zostra marina comprises up to 46% of the Great Bay subtidal habitat, R. maritima`s much smaller size makes it a more practical laboratory organism. Effects on Ruppia may offer useful insights into potential effects on Zostra or other aquatic vascular plants. Presently rooted vascular plants are not found in Clark Cove located adjacent to a landfill disposal site on the shipyard. The absence of rooted vegetation can be contributed to, physical parameters of the site (turbidity, grain size, texture) or chemical parameters (heavy metal/Pb contamination, redox potential). Exposure of bedded and nonbedded plants occurred over a four day and ten day period using lead sulfate. Concentrations for bedded exposures were as follows, 0.3, 0.5, 0.8, 1.0 simultaneously extracted metal/acid volatile sulfide (SEM/AVS) molar ratios, and 0.1, 1.0, 10.0 and 100.0mg/l Pb for water only exposures. Reduction in cumulative leaf growth was observed for the Clark Cove sediments as well as the spiked sediments as compared to reference sediments.

  6. Comparative assessment of LECA and Spartina maritima to remove emerging organic contaminants from wastewater.

    Science.gov (United States)

    Ferreira, Ana Rita; Guedes, Paula; Mateus, Eduardo P; Ribeiro, Alexandra B; Couto, Nazaré

    2017-03-01

    The present work aimed to evaluate the capacity of constructed wetlands (CWs) to remove three emerging organic contaminants with different physicochemical properties: caffeine (CAF), oxybenzone (MBPh), and triclosan (TCS). The simulated CWs were set up with a matrix of light expanded clay aggregates (LECA) and planted with Spartina maritima, a salt marsh plant. Controlled experiments were carried out in microcosms using deionized water and wastewater collected at a wastewater treatment plant (WWTP), with different contaminant mass ranges, for 3, 7, and 14 days. The effects of variables were tested isolatedly and together (LECA and/or S. maritima). The presence of LECA and/or S. maritima has shown higher removal (around 61-97%) of lipophilic compounds (MBPh and TCS) than the hydrophilic compound (CAF; around 19-85%). This was attributed to the fact that hydrophilic compounds are dissolved in the water column, whereas the lipophilic ones suffer sorption processes promoting their removal by plant roots and/or LECA. In the control (only wastewater), a decrease in the three contaminant levels was observed. Adsorption and bio/rhizoremediation are the strongest hypothesis to explain the decrease in contaminants in the tested conditions.

  7. Contribution of Spartina maritima to the reduction of eutrophication in estuarine systems

    Energy Technology Data Exchange (ETDEWEB)

    Sousa, Ana I. [IO - Institute of Oceanography, Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisbon (Portugal); IMAR - Institute of Marine Research, Department of Zoology, University of Coimbra, 3004-517 Coimbra (Portugal)], E-mail: aisousa@fc.ul.pt; Lillebo, Ana I. [CESAM - Centro de Estudos do Ambiente e do Mar, Department of Chemistry, University of Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Cacador, Isabel [IO - Institute of Oceanography, Faculty of Sciences, University of Lisbon, Campo Grande, 1749-016 Lisbon (Portugal); Pardal, Miguel A. [IMAR - Institute of Marine Research, Department of Zoology, University of Coimbra, 3004-517 Coimbra (Portugal)

    2008-12-15

    Salt marshes are among the most productive ecosystems in the world, performing important ecosystem functions, particularly nutrient recycling. In this study, a comparison is made between Mondego and Tagus estuaries in relation to the role of Spartina maritima in nitrogen retention capacity and cycling. Two mono-specific S. maritima stands per estuary were studied during 1 yr (biomass, nitrogen (N) pools, litter production, decomposition rates). Results showed that the oldest Tagus salt marsh population presented higher annual belowground biomass and N productions, and a slower decomposition rate for litter, contributing to the higher N accumulation in the sediment, whereas S. maritima younger marshes had higher aboveground biomass production. Detritus moved by tides represented a huge amount of aboveground production, probably significant when considering the N balance of these salt marshes. Results reinforce the functions of salt marshes as contributing to a reduction of eutrophication in transitional waters, namely through sedimentation processes. - The crucial capacity of salt marshes to retain nitrogen, thus reducing eutrophication, greatly depends on the salt marsh maturity, rather than the estuarine system.

  8. Antinociceptive, anti-inflammatory and antioxidant activities of aqueous extract from Remirea maritima (Cyperaceae).

    Science.gov (United States)

    Rabelo, A S; Oliveira, I D; Guimarães, A G; Quintans, J S S; Prata, A P N; Gelain, D P; Venceslau, E M; Santos, J P A; Quintans, L J; Bonjardim, L R; Barison, A; Campos, F R; Santos, A D C; Nogueira, P C L; Costa, E V; Moraes, V R S; Araújo, A A S

    2013-01-09

    Remirea maritima Aubl., popularly known as "capim-da-praia", is popularly employed in the treatment of diarrhea, kidney disease, fever, and for analgesic and anti-inflammatory purposes through the preparation of teas. Few studies have focused on the chemical composition and its biological properties. This work evaluated the antinocipetive, anti-inflammatory and antioxidant activities of the aqueous extract from Remirea maritima Aubl. as well as the isolation and identification of the chemical compounds. Compounds were isolated from aqueous extract of Remirea maritima through preparative HPLC and the structures were identified by means of NMR and MS analysis. The tests for antinociceptive, anti-inflammatory, and antioxidant activities, along with motor coordination test (Rota rod), were performed over the aqueous extract. The phytochemical investigation of aqueous extract of Remirea maritima resulted in the isolation of three flavone glycosides. The structures of these compounds were determined by means of MS and 1D and 2D NMR data as vitexin-2″-O-β-D-glucopyranoside, isovitexin-2″-O-β-D-glucopyranoside, and luteolin-7-O-glucuronide. Acute pretreatment with aqueous extract (100, 200 or 400mg/kg, i.p.) caused a significant decrease (p<0.001) in the number of abdominal writhes. In the formalin test, higher doses significantly inhibited the late (inflammatory pain) phase of formalin-induced licking (p<0.05 or 0.001). In the hot plate test, there was no significant difference in nociceptive behavior, discarding the possible central effect of the aqueous extract. In the rota rod test, it was verified that the aqueous extract in all concentration evaluated does not alter the motor coordination of mice, such antinociceptive results were unlikely to be caused by motor abnormality. In the peritonitis test, induced by carrageenan, the treatment with aqueous extract produced a significant reduction in leukocyte migration in all concentration evaluated. Additionally, a

  9. Thermal-induced conformational changes in the product release area drive the enzymatic activity of xylanases 10B: Crystal structure, conformational stability and functional characterization of the xylanase 10B from Thermotoga petrophila RKU-1

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Camila Ramos; Meza, Andreia Navarro [Laboratorio Nacional de Biociencias (LNBio), Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP (Brazil); Hoffmam, Zaira Bruna; Silva, Junio Cota; Alvarez, Thabata Maria; Ruller, Roberto [Laboratorio Nacional de Ciencia e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP (Brazil); Giesel, Guilherme Menegon; Verli, Hugo [Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS (Brazil); Squina, Fabio Marcio [Laboratorio Nacional de Ciencia e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP (Brazil); Prade, Rolf Alexander [Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK (United States); Murakami, Mario Tyago, E-mail: mario.murakami@lnbio.org.br [Laboratorio Nacional de Biociencias (LNBio), Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP (Brazil)

    2010-12-10

    Research highlights: {yields} The hyperthermostable xylanase 10B from Thermotoga petrophila RKU-1 produces exclusively xylobiose at the optimum temperature. {yields} Circular dichroism spectroscopy suggests a coupling effect of temperature-induced structural changes with its enzymatic behavior. {yields} Crystallographic and molecular dynamics studies indicate that conformational changes in the product release area modulate the enzyme action mode. -- Abstract: Endo-xylanases play a key role in the depolymerization of xylan and recently, they have attracted much attention owing to their potential applications on biofuels and paper industries. In this work, we have investigated the molecular basis for the action mode of xylanases 10B at high temperatures using biochemical, biophysical and crystallographic methods. The crystal structure of xylanase 10B from hyperthermophilic bacterium Thermotoga petrophila RKU-1 (TpXyl10B) has been solved in the native state and in complex with xylobiose. The complex crystal structure showed a classical binding mode shared among other xylanases, which encompasses the -1 and -2 subsites. Interestingly, TpXyl10B displayed a temperature-dependent action mode producing xylobiose and xylotriose at 20 {sup o}C, and exclusively xylobiose at 90 {sup o}C as assessed by capillary zone electrophoresis. Moreover, circular dichroism spectroscopy suggested a coupling effect of temperature-induced structural changes with this particular enzymatic behavior. Molecular dynamics simulations supported the CD analysis suggesting that an open conformational state adopted by the catalytic loop (Trp297-Lys326) provokes significant modifications in the product release area (+1,+2 and +3 subsites), which drives the enzymatic activity to the specific release of xylobiose at high temperatures.

  10. The first myriapod genome sequence reveals conservative arthropod gene content and genome organisation in the centipede Strigamia maritima.

    Directory of Open Access Journals (Sweden)

    Ariel D Chipman

    2014-11-01

    Full Text Available Myriapods (e.g., centipedes and millipedes display a simple homonomous body plan relative to other arthropods. All members of the class are terrestrial, but they attained terrestriality independently of insects. Myriapoda is the only arthropod class not represented by a sequenced genome. We present an analysis of the genome of the centipede Strigamia maritima. It retains a compact genome that has undergone less gene loss and shuffling than previously sequenced arthropods, and many orthologues of genes conserved from the bilaterian ancestor that have been lost in insects. Our analysis locates many genes in conserved macro-synteny contexts, and many small-scale examples of gene clustering. We describe several examples where S. maritima shows different solutions from insects to similar problems. The insect olfactory receptor gene family is absent from S. maritima, and olfaction in air is likely effected by expansion of other receptor gene families. For some genes S. maritima has evolved paralogues to generate coding sequence diversity, where insects use alternate splicing. This is most striking for the Dscam gene, which in Drosophila generates more than 100,000 alternate splice forms, but in S. maritima is encoded by over 100 paralogues. We see an intriguing linkage between the absence of any known photosensory proteins in a blind organism and the additional absence of canonical circadian clock genes. The phylogenetic position of myriapods allows us to identify where in arthropod phylogeny several particular molecular mechanisms and traits emerged. For example, we conclude that juvenile hormone signalling evolved with the emergence of the exoskeleton in the arthropods and that RR-1 containing cuticle proteins evolved in the lineage leading to Mandibulata. We also identify when various gene expansions and losses occurred. The genome of S. maritima offers us a unique glimpse into the ancestral arthropod genome, while also displaying many adaptations

  11. The First Myriapod Genome Sequence Reveals Conservative Arthropod Gene Content and Genome Organisation in the Centipede Strigamia maritima

    Science.gov (United States)

    Chipman, Ariel D.; Ferrier, David E. K.; Brena, Carlo; Qu, Jiaxin; Hughes, Daniel S. T.; Schröder, Reinhard; Torres-Oliva, Montserrat; Znassi, Nadia; Jiang, Huaiyang; Almeida, Francisca C.; Alonso, Claudio R.; Apostolou, Zivkos; Aqrawi, Peshtewani; Arthur, Wallace; Barna, Jennifer C. J.; Blankenburg, Kerstin P.; Brites, Daniela; Capella-Gutiérrez, Salvador; Coyle, Marcus; Dearden, Peter K.; Du Pasquier, Louis; Duncan, Elizabeth J.; Ebert, Dieter; Eibner, Cornelius; Erikson, Galina; Evans, Peter D.; Extavour, Cassandra G.; Francisco, Liezl; Gabaldón, Toni; Gillis, William J.; Goodwin-Horn, Elizabeth A.; Green, Jack E.; Griffiths-Jones, Sam; Grimmelikhuijzen, Cornelis J. P.; Gubbala, Sai; Guigó, Roderic; Han, Yi; Hauser, Frank; Havlak, Paul; Hayden, Luke; Helbing, Sophie; Holder, Michael; Hui, Jerome H. L.; Hunn, Julia P.; Hunnekuhl, Vera S.; Jackson, LaRonda; Javaid, Mehwish; Jhangiani, Shalini N.; Jiggins, Francis M.; Jones, Tamsin E.; Kaiser, Tobias S.; Kalra, Divya; Kenny, Nathan J.; Korchina, Viktoriya; Kovar, Christie L.; Kraus, F. Bernhard; Lapraz, François; Lee, Sandra L.; Lv, Jie; Mandapat, Christigale; Manning, Gerard; Mariotti, Marco; Mata, Robert; Mathew, Tittu; Neumann, Tobias; Newsham, Irene; Ngo, Dinh N.; Ninova, Maria; Okwuonu, Geoffrey; Ongeri, Fiona; Palmer, William J.; Patil, Shobha; Patraquim, Pedro; Pham, Christopher; Pu, Ling-Ling; Putman, Nicholas H.; Rabouille, Catherine; Ramos, Olivia Mendivil; Rhodes, Adelaide C.; Robertson, Helen E.; Robertson, Hugh M.; Ronshaugen, Matthew; Rozas, Julio; Saada, Nehad; Sánchez-Gracia, Alejandro; Scherer, Steven E.; Schurko, Andrew M.; Siggens, Kenneth W.; Simmons, DeNard; Stief, Anna; Stolle, Eckart; Telford, Maximilian J.; Tessmar-Raible, Kristin; Thornton, Rebecca; van der Zee, Maurijn; von Haeseler, Arndt; Williams, James M.; Willis, Judith H.; Wu, Yuanqing; Zou, Xiaoyan; Lawson, Daniel; Muzny, Donna M.; Worley, Kim C.; Gibbs, Richard A.; Akam, Michael; Richards, Stephen

    2014-01-01

    Myriapods (e.g., centipedes and millipedes) display a simple homonomous body plan relative to other arthropods. All members of the class are terrestrial, but they attained terrestriality independently of insects. Myriapoda is the only arthropod class not represented by a sequenced genome. We present an analysis of the genome of the centipede Strigamia maritima. It retains a compact genome that has undergone less gene loss and shuffling than previously sequenced arthropods, and many orthologues of genes conserved from the bilaterian ancestor that have been lost in insects. Our analysis locates many genes in conserved macro-synteny contexts, and many small-scale examples of gene clustering. We describe several examples where S. maritima shows different solutions from insects to similar problems. The insect olfactory receptor gene family is absent from S. maritima, and olfaction in air is likely effected by expansion of other receptor gene families. For some genes S. maritima has evolved paralogues to generate coding sequence diversity, where insects use alternate splicing. This is most striking for the Dscam gene, which in Drosophila generates more than 100,000 alternate splice forms, but in S. maritima is encoded by over 100 paralogues. We see an intriguing linkage between the absence of any known photosensory proteins in a blind organism and the additional absence of canonical circadian clock genes. The phylogenetic position of myriapods allows us to identify where in arthropod phylogeny several particular molecular mechanisms and traits emerged. For example, we conclude that juvenile hormone signalling evolved with the emergence of the exoskeleton in the arthropods and that RR-1 containing cuticle proteins evolved in the lineage leading to Mandibulata. We also identify when various gene expansions and losses occurred. The genome of S. maritima offers us a unique glimpse into the ancestral arthropod genome, while also displaying many adaptations to its specific

  12. Hydrogen production from carrot pulp by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    Energy Technology Data Exchange (ETDEWEB)

    Vrije, Truus de; Budde, Miriam A.W.; Lips, Steef J.; Bakker, Robert R.; Mars, Astrid E.; Claassen, Pieternel A.M. [Wageningen UR, Food and Biobased Research, P.O. Box 17, 6700 AA Wageningen (Netherlands)

    2010-12-15

    Hydrogen was produced from carrot pulp hydrolysate, untreated carrot pulp and (mixtures of) glucose and fructose by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana in pH-controlled bioreactors. Carrot pulp hydrolysate was obtained after enzymatic hydrolysis of the polysaccharide fraction in carrot pulp. The main sugars in the hydrolysate were glucose, fructose, and sucrose. In fermentations with glucose hydrogen yields and productivities were similar for both strains. With fructose the hydrogen yield of C. saccharolyticus was reduced which might be related to uptake of glucose and fructose by different types of transport systems. With T. neapolitana the fructose consumption rate and consequently the hydrogen productivity were low. The hydrogen yields of both thermophiles were 2.7-2.8 mol H{sub 2}/mol hexose with 10 g/L sugars from carrot pulp hydrolysate. With 20 g/L sugars the yield of T. neapolitana was 2.4 mol H{sub 2}/mol hexose while the yield of C. saccharolyticus was reduced to 1.3 mol H{sub 2}/mol hexose due to high lactate production in the stationary growth phase. C. saccharolyticus was able to grow on carrot pulp and utilized soluble sugars and, after adaptation, pectin and some (hemi)cellulose. No growth was observed with T. neapolitana when using carrot pulp in agitated fermentations. Enzymatic hydrolysis of the polysaccharide fraction prior to fermentation increased the hydrogen yield with almost 10% to 2.3 g/kg of hydrolyzed carrot pulp. (author)

  13. Perianth development in the basal monocot Triglochin maritima (Juncaginaceae)

    DEFF Research Database (Denmark)

    Buzgo, Matyas; Soltis, Douglas E.; Soltis, Pamela S.

    2006-01-01

    Basal monocots exhibit considerable variation in inflorescence and floral structure. In some cases, such as Triglochin maritima, it is not clear whether the lateral and terminal structures of the inflorescence are flowers or pseudanthia, or where the limits between flowers and inflorescence lie.......'' Therefore, in some basal angiosperms with abbreviated development of lateral flowers the demarcation of the flower vs. the inflorescence is ontogenetically ambiguous. In situ hybridization experiments show that a putative ortholog of the B-class gene APETALA3/DEFICIENS is expressed in developing stamens...

  14. "Tyrichae" al riu Sénia. Rellegint l' "Ora Maritima" d'Aviè

    Directory of Open Access Journals (Sweden)

    David Garcia i Rubert

    2008-01-01

    Full Text Available The works of archaeological research developed in the last years on the territories of the Ebre and Sénia rivers, located straddling Catalonia and the Valencian Country, have been intense and significant. The advances obtained in the knowledge of the protohistoric communities of the zone allow us to carry out a new reading of Ora Maritima from Avienus. In a more specific way, in this work we explore the possibility to assimilate the toponym Tyrichae, mentioned in this text, with one of the settlements placed on this territory.

  15. Anthemis maritima L. in different coastal habitats: A tool to explore plant plasticity

    Science.gov (United States)

    Spanò, Carmelina; Balestri, Mirko; Bottega, Stefania; Grilli, Isa; Forino, Laura Maria Costantina; Ciccarelli, Daniela

    2013-09-01

    Anthemis maritima, a plant which has the ability to colonise different stressful coastal environments, sand dunes and rocky cliff ecosystems, exhibits a high degree of leaf trait plasticity. The key parameters are the regulation of stomatal density and size, the succulence index and the specific antioxidant response. With the aim to explore plant plasticity, we analysed various morphological and physiological traits of the leaves of A. maritima populations dwelling in three different coastal areas of Italy. The highest values of stomatal density, leaf thickness, and succulence index were found in plants living in a sub-arid climate, on rocky cliffs, with the highest soil pH and salinity. Although this population exhibited the highest concentration of oxygen reactive species (hydrogen peroxide), it also had the lowest value of lipid peroxidation, an indicator of oxidative stress. Ascorbate was the main protective molecule in this population, while phenols appeared to carry out this role in plants living on soils with the lowest salinity and highest annual rainfall.

  16. The protective role of damsissa (Ambroosia Maritima) against gamma irradiation in albino rats

    International Nuclear Information System (INIS)

    Osman, O.A.; Mohamed, Y.S.

    2003-01-01

    The present work was directed to evaluate the effectiveness of treatment with damsissa (Ambrosia maritima) for thirty consecutive days pre- irradiation exposure in controlling the post-irradiation hazards in irradiated rats. Male albino rats (Spraue Dowley strain) weighing about 120+- 10 g were used and blood samples were collected from tails of animals thirty days after treatment with damsissa and seven days post irradiation. Blood samples were subjected to biochemical analysis such as liver functions, kidney function and lipid profile. Whole body gamma irradiation of rats at 6 Gy (single dose) caused significant decrease in the contents of total proteins accompanied by significant increase of urea level as recorded on the 7th days post irradiation. Data obtained in this study revealed that whole body gamma irradiation induced significant elevation in all tested blood lipid functions. There was significant increase of aspartate amino transferase (AST) and alanine amino transferase (ALT) whole alkaline phosphatase (ALP) showed statistical significant decrease as compared with the control group. Damisissa (Ambrosia maritima) treatment exerted noticeable amelioration in the the studied biochemical parameters of the irradiated albino rats. The mechanism of action of damsissa may be due to its anti-inflammatory properties against whole body gamma irradiation

  17. Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

    Energy Technology Data Exchange (ETDEWEB)

    Almeida Gadelha, Carlos Alberto de [BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970 (Brazil); Moreno, Frederico Bruno Mendes Batista [Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000 (Brazil); Santi-Gadelha, Tatiane; Cajazeiras, João Batista; Rocha, Bruno Anderson M. da [BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970 (Brazil); Rustiguel, Joane Kathelen Rodrigues [Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000 (Brazil); Freitas, Beatriz Tupinamba [BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970 (Brazil); Grupo de Química Biológica, Departamento de Ciências Biológicas, Universidade Regional do Cariri, Crato, CE 63195-000 (Brazil); Canduri, Fernanda [Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000 (Brazil); Delatorre, Plínio [BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970 (Brazil); Grupo de Química Biológica, Departamento de Ciências Biológicas, Universidade Regional do Cariri, Crato, CE 63195-000 (Brazil); Azevedo, Walter Filgueira Jr de, E-mail: walterfa@df.ibilce.unesp.br [Programa de Pós-graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000 (Brazil); Cavada, Benildo S., E-mail: walterfa@df.ibilce.unesp.br [BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970 (Brazil)

    2005-01-01

    A lectin from C. maritima was crystallized using the vapour-diffusion method and crystals diffracted to 2.1 Å resolution. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%, refinement is in progress. A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 Å resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2{sub 1}2{sub 1}2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 Å. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.

  18. Effect of Gamma Rays and Salinity on Growth and Chemical Composition of Ambrosia maritima L. Plant

    International Nuclear Information System (INIS)

    Moemen, A.M.E.

    2012-01-01

    This work achieved to study the effects of, mixture of salt 2:2:1 (Na Cl-CaCl 2 and Mg SO 4 ), concentration of (0, 2000, 4000 and 6000 ppm). on growth characters, some chemical components and some active ingredients in shoots of Ambrosia maritima plants, at different stages of growth, during two seasons. Pots 30 cm in diameter were filled of sand-loamy soils in appropriate concentration, all pots were irrigated with tap water. The exposed damsisa seeds to gamma rays, doses (0, 20, 40, and 80 Gy) before sowing together with control non irradiated seeds were sown in saline soils (0, 2000, 4000 and 6000 ppm). Soil salinity treatments caused a decrease in plant height, number of leaves, content of damsin, and an increase in fresh weigh, dry weight, total sugars, total chlorophyll, amino acids and ambrosine content. Also, Gamma rays caused an increase in most of growth parameters and most of chemical composition. It was observed that 40 or 80 Gy was more effective. We investigated the combined effect of levels of salinity and doses of radiation used, this interference improve growth parameters and chemical composition in ambrosia maritima plants and caused ascertain the role of gamma irradiation in plants tolerance to soil salinity and alleviation their harmful effect on plants.

  19. Biophysical probing of Spartina maritima photo-system II changes during prolonged tidal submersion periods.

    Science.gov (United States)

    Duarte, B; Santos, D; Marques, J C; Caçador, I

    2014-04-01

    Submergence is one of the major constrains affecting wetland plants, with inevitable impacts on their physiology and productivity. Global warming as a driving force of sea level rise, tend to increase the submersion periods duration. Photosynthesis biophysical probing arise as an important tool to understand the energetics underlying plant feedback to these constrains. As in previous studies with Spartina maritima, there was no inhibition of photosynthetic activity in submerged individuals. Comparing both donor and acceptor sides of the PSII, the first was more severely affected during submersion, driven by the inactivation of the OEC with consequent impairment of the ETC. Although this apparent damage in the PSII donor side, the electron transport per active reaction centre was not substantially affected, indicating that this reduction in the electron flow is accompanied by a proportional increase in the number of active reaction centres. These conditions lead to the accumulation of excessive reducing power, source of damaging ROS, counteracted by efficient energy dissipation processes and anti-oxidant enzymatic defences. This way, S. maritima appears as a well-adapted species with an evident photochemical plasticity towards submersion, allowing it to maintain its photosynthetic activity even during prolonged submersion periods. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  20. The centipede Strigamia maritima: what it can tell us about the development and evolution of segmentation.

    Science.gov (United States)

    Arthur, Wallace; Chipman, Ariel D

    2005-06-01

    One of the most fundamental features of the body plan of arthropods is its segmental design. There is considerable variation in segment number among arthropod groups (about 20-fold); yet, paradoxically, the vast majority of arthropod species have a fixed number of segments, thus providing no variation in this character for natural selection to act upon. However, the 1000-species-strong centipede order Geophilomorpha provides an exception to the general rule of intraspecific invariance in segment number. Members of this group, and especially our favourite animal Strigamia maritima, may thus help us to understand the evolution of segment number in arthropods. Evolution must act by modifying the formation of segments during embryogenesis. So, how this developmental process operates, in a variable-segment-number species, is of considerable interest. Strigamia maritima turns out to be a tractable system both at the ecological level of investigating differences in mean segment number between populations and at the molecular level of studying the expression patterns of developmental genes. Here we report the current state of play in our work on this fascinating animal, including our recent finding of a double-segment periodicity in the expression of two Strigamia segmentation genes, and its possible implications for our understanding of arthropod segmentation mechanisms in general.

  1. Chemical composition and biological effects of Artemisia maritima and Artemisia nilagirica essential oils from wild plant of Western Himalaya

    Science.gov (United States)

    Artemisia species possess pharmacological properties that are used for medical purposes worldwide. In this paper, the essential oils from the aerial parts of A. nilagirica and A. maritima from the western Indian Himalaya region are described. The main compounds analyzed by simultaneous GC/MS and GC/...

  2. Enhancement of fermentative hydrogen production from green algal biomass of Thermotoga neapolitana by various pretreatment methods

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, Tam-Anh D.; Kim, Kyoung-Rok; Nguyen, Minh-Thu; Sim, Sang Jun [Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Kim, Mi Sun [Bioenergy Research Center, Korea Institute of Energy Research, Daejeon 305-343 (Korea, Republic of); Kim, Donhue [Department of Biochemical Engineering, Dongyang Mirae College, Seoul 152-714 (Korea, Republic of)

    2010-12-15

    Biomass of the green algae has been recently an attractive feedstock source for bio-fuel production because the algal carbohydrates can be derived from atmospheric CO{sub 2} and their harvesting methods are simple. We utilized the accumulated starch in the green alga Chlamydomonas reinhardtii as the sole substrate for fermentative hydrogen (H{sub 2}) production by the hyperthermophilic eubacterium Thermotoga neapolitana. Because of possessing amylase activity, the bacterium could directly ferment H{sub 2} from algal starch with H{sub 2} yield of 1.8-2.2 mol H{sub 2}/mol glucose and the total accumulated H{sub 2} level from 43 to 49% (v/v) of the gas headspace in the closed culture bottle depending on various algal cell-wall disruption methods concluding sonication or methanol exposure. Attempting to enhance the H{sub 2} production, two pretreatment methods using the heat-HCl treatment and enzymatic hydrolysis were applied on algal biomass before using it as substrate for H{sub 2} fermentation. Cultivation with starch pretreated by 1.5% HCl at 121 C for 20 min showed the total accumulative H{sub 2} yield of 58% (v/v). In other approach, enzymatic digestion of starch by thermostable {alpha}-amylase (Termamyl) applied in the SHF process significantly enhanced the H{sub 2} productivity of the bacterium to 64% (v/v) of total accumulated H{sub 2} level and a H{sub 2} yield of 2.5 mol H{sub 2}/mol glucose. Our results demonstrated that direct H{sub 2} fermentation from algal biomass is more desirably potential because one bacterial cultivation step was required that meets the cost-savings, environmental friendly and simplicity of H{sub 2} production. (author)

  3. Obligate sugar oxidation in Mesotoga spp., phylum Thermotogae, in the presence of either elemental sulfur or hydrogenotrophic sulfate-reducers as electron acceptor.

    Science.gov (United States)

    Fadhlaoui, Khaled; Ben Hania, Wagdi; Armougom, Fabrice; Bartoli, Manon; Fardeau, Marie-Laure; Erauso, Gaël; Brasseur, Gaël; Aubert, Corinne; Hamdi, Moktar; Brochier-Armanet, Céline; Dolla, Alain; Ollivier, Bernard

    2018-01-01

    Mesotoga prima strain PhosAc3 is a mesophilic representative of the phylum Thermotogae comprising only fermentative bacteria so far. We show that while unable to ferment glucose, this bacterium is able to couple its oxidation to reduction of elemental sulfur. We demonstrate furthermore that M. prima strain PhosAc3 as well as M. prima strain MesG1 and Mesotoga infera are able to grow in syntrophic association with sulfate-reducing bacteria (SRB) acting as hydrogen scavengers through interspecies hydrogen transfer. Hydrogen production was higher in M. prima strain PhosAc3 cells co-cultured with SRB than in cells cultured alone in the presence of elemental sulfur. We propose that the efficient sugar-oxidizing metabolism by M. prima strain PhosAc3 in syntrophic association with a hydrogenotrophic sulfate-reducing bacterium can be extrapolated to all members of the Mesotoga genus. Genome comparison of Thermotogae members suggests that the metabolic difference between Mesotoga and Thermotoga species (sugar oxidation versus fermentation) is mainly due to the absence of the bifurcating [FeFe]-hydrogenase in the former. Such an obligate oxidative process for using sugars, unusual within prokaryotes, is the first reported within the Thermotogae. It is hypothesized to be of primary ecological importance for growth of Mesotoga spp. in the environments that they inhabit. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  4. Reproductive Biology of Mangrove Plants Clerodendrum inerme, Derris trifoliata, Suaeda maritima, Suaeda monoica, Suaeda nudiflora

    Directory of Open Access Journals (Sweden)

    Aluri Jacob Solomon Raju

    2016-12-01

    Full Text Available Floral biology, sexual system, breeding system, pollinators, fruiting and seed dispersal aspects of five mangrove associate species, namely, Clerodendrum inerme, Derris trifoliata, Suaeda maritima, S. monoica, and S. nudiflora were studied. All these species are hermaphroditic, self-compatible, and exhibit mixed breeding systems adapted for pollination by external agents. C. inerme and D. trifoliata are principally entomophilous, while Suaeda species are ambophilous. The study suggests that these plant species are important constituents of mangrove forests. C. inerme and D. trifoliata are useful in stabilizing the banks of back water creeks, while Suaeda species are useful to carpet the saline areas and desalinate the soils which are subsequently useful for agricultural activities.

  5. Cloning, purification, and characterization of xylose isomerase from Thermotoga naphthophila RKU-10.

    Science.gov (United States)

    Fatima, Bilqees; Aftab, Muhammad Nauman; Haq, Ikram-Ul

    2016-09-01

    A 1.3 kb xyl-A gene encoding xylose isomerase from a hyperthermophilic eubacterium Thermotoga naphthophila RKU-10 (TnapXI) was cloned and over-expressed in Escherichia coli to produce the enzyme in mesophilic conditions that work at high temperature. The enzyme was concentrated by lyophilization and purified by heat treatment, fractional precipitation, and UNOsphere Q anion-exchange column chromatography to homogeneity level. The apparent molecular mass was estimated by SDS-PAGE to be 49.5 kDa. The active enzyme showed a clear zone on Native-PAGE when stained with 2, 3, 5-triphenyltetrazolium chloride. The optimum temperature and pH for D-glucose to D-fructose isomerization were 98 °C and 7.0, respectively. Xylose isomerase retains 85% of its activity at 50 °C (t1/2 1732 min) for 4 h and 32.5% at 90 °C (t1/2 58 min) for 2 h. It retains 90-95% of its activity at pH 6.5-7.5 for 30 min. The enzyme was highly activated (350%) with the addition of 0.5 mM Co(2+) and to a lesser extent about 180 and 80% with the addition of 5 and 10 mM Mn(2+) and Mg(2+) , respectively but it was inhibited (54-90%) in the presence of 0.5-10 mM Ca(2+) with respect to apo-enzyme. D-glucose isomerization product was also analyzed by Thin Layer Chromatography (Rf 0.65). The enzyme was very stable at neutral pH and sufficiently high temperature and required only a trace amount of Co(2+) for its optimal activity and stability. Overall, 52.2% conversion of D-glucose to D-fructose was achieved by TnapXI. Thus, it has a great potential for industrial applications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Proteomic and metabolic profiles of Cakile maritima Scop. Sea Rocket grown in the presence of cadmium.

    Science.gov (United States)

    Taamalli, Manel; D'Alessandro, Angelo; Marrocco, Cristina; Gevi, Federica; Timperio, Anna Maria; Zolla, Lello

    2015-04-01

    Recent physiological reports have documented how Cakile maritima Scop. Sea Rocket could accumulate high doses of Cd without altering its physiological parameters. In the present study, we performed an integrated proteomics (2DE) and metabolomics (HPLC-MS) investigation to determine the molecular mechanisms underlying cadmium (Cd) tolerance of this halophyte. Peculiar features were observed: (i) up-regulation of thiol compound anabolism, including glutathione and phytochelatin homeostasis, which allows an intracellular chelation of Cd and its compartmentalization into vacuole by a significant up-regulation of vacuolar transporters; (ii) up-regulation of the PPP and Calvin cycle (both at the enzyme and metabolite level), which utterly promoted the maintenance of NADPH/NADP(+) homeostasis, other than the accumulation of triose-phosphates (serving as anabolic intermediates for triacylglycerol biosynthesis) and the glyoxylate precursor phosphoglycolate, to promote photorespiration and consequently CO2 release. An up-regulation of carbonic anhydrase was also observed. This halophyte is also correlated with a highly efficient antioxidant system, especially a high up-regulation of SOD1, resulting more efficient in coping with heavy metals stress than common plants. Interestingly, exposure to high Cd concentrations partly affected photosystem integrity and metabolic activity, through the up-regulation of enzymes from the Calvin cycle and glutathione-ascorbate homeostasis and PAP3 which stabilizes thylakoid membrane structures. In addition, up-regulation of Peptidyl-prolyl isomerase CYP38 increases stability and biogenesis of PSII. Finally, metabolomics results confirmed proteomics and previous physiological evidence, also suggesting that osmoprotectants, betaine and proline, together with plant hormones, methyl jasmonate and salicylic acid, might be involved in mediating responses to Cd-induced stress. Taken together, these peculiar features confirm that Cakile maritima

  7. Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast

    DEFF Research Database (Denmark)

    Erbasol, Isil; Bozdag, Gonensin Ozan; Koc, Ahmet

    2013-01-01

    Manganese (Mn) is an essential micronutrient in plants. However increased Mn levels are toxic to plant cells. Metal tolerance proteins (MTPs), member of cation diffusion facilitator protein (CDF) family, have important roles in metal homeostatis in different plant species and catalyse efflux...... in planta localization and function as the Arabidopsis Mn-CDF homolog AtMTP11 and this conservation shows the evolutionary importance of these vesicular proteins in heavy metal homeostatis among plant species....... of excess metal ions. In this study, we identified and characterized two MTP genes from Beta vulgaris spp. maritima (B. v. ssp. maritima). Overexpression of these two genes provided Mn tolerance in yeast cells. Sequence analyses displayed BmMTP10 and BmMTP11as members of the Mn-CDF family. Functional...

  8. Effets de dix traitements sur la germination des akènes d'Ambrosia maritima L

    Directory of Open Access Journals (Sweden)

    Schafer, JL.

    1989-01-01

    Full Text Available Effects of ten treatments on the germination of Ambrosia maritima L. seeds. Stratification of Ambrosia maritima seeds at + 10°C for 7 days appeared to be the best practical method to break their embryonic dormancy with 80 % germination occuring 18 days following the treatment, compared to 17 % for the control. This experiment confirms the susceptibility of embryonic dormancy to low temperatures. Treatments with waterat + 80°C or concentrated H2S04 for 15 mn and mechanical treatments eliminating the inhibition effect of seed integuments also gave higher results (43 to 53 % germination rate. However, germination in the control group was significantly higher than in the group of seeds subjected to dry heat. Although the first results obtained on the field from these trials were satisfactory, further research is needed to confirm them.

  9. Genetic Diversity and Physiological Performance of Portuguese Wild Beet (Beta vulgaris spp. maritima) from Three Contrasting Habitats

    OpenAIRE

    Ribeiro, Isa C.; Pinheiro, Carla; Ribeiro, Carla M.; Veloso, Maria M.; Simoes-Costa, Maria C.; Evaristo, Isabel; Paulo, Oct?vio S.; Ricardo, C?ndido P.

    2016-01-01

    The establishment of stress resilient sugar beets (Beta vulgaris spp. vulgaris) is an important breeding goal since this cash crop is susceptible to drought and salinity. The genetic diversity in cultivated sugar beets is low and the beet wild relatives are useful genetic resources for tolerance traits. Three wild beet populations (Beta vulgaris spp. maritima) from contrasting environments, Vaiamonte (VMT, dry inland hill), Comporta (CMP, marsh) and Oeiras (OEI, coastland), and...

  10. Genetic diversity and physiological performance of Portuguese wild beet (Beta vulgaris spp. maritima) from three contrasting habitats

    OpenAIRE

    Isa Catarina Ribeiro; Carla Pinheiro; Carla Pinheiro; Carla Maria Ribeiro; Maria Manuela Veloso; Maria Manuela Veloso; Maria Cristina Simões-Costa; Isabel Evaristo; Octávio Salgueiro Paulo; Cândido Pinto Ricardo

    2016-01-01

    The establishment of stress resilient sugar beets (Beta vulgaris spp. vulgaris) is an important breeding goal since this cash crop is susceptible to drought and salinity. The genetic diversity in cultivated sugar beets is low and the beet wild relatives are useful genetic resources for tolerance traits. Three wild beet populations (Beta vulgaris spp. maritima) from contrasting environments, Vaiamonte (VMT, dry inland hill), Comporta (CMP, marsh) and Oeiras (OEI, coastland), and one commercial...

  11. [Construction and characterization of a recombinant mutant homolog of the CheW protein from Thermotoga petrophila RKU-1].

    Science.gov (United States)

    Grishin, D V; Zhdanov, D D; Gladilina, Ju A; Pokrovsky, V S; Podobed, O V; Pokrovskaya, M V; Aleksandrova, S S; Milyushkina, A L; Vigovskiy, M A; Sokolov, N N

    2018-01-01

    In the work a recombinant chemotaxis protein CheW from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homolog (TpeCheW-mut) were created. It was shown that, despite the low homology with CheW prototypes from intestinal bacteria, these proteins didn't cause metabolic overload and were well expressed by cells of E. coli laboratory strains. We have discovered a broad spectrum of industrial valuable properties of the TpeCheW-mut protein such as stability in a wide range of temperatures and pH, high expression level, solubility and possibility of the application of a simple low-stage purification methodology with the use of preliminary heat treatment. Possible directions of the scientific and industrial application of this protein were claimed.

  12. Endophytic cultivable bacteria of the metal bioaccumulator Spartina maritima improve plant growth but not metal uptake in polluted marshes soils

    Directory of Open Access Journals (Sweden)

    Jenifer eMesa

    2015-12-01

    Full Text Available Endophytic bacterial population was isolated from Spartina maritima tissues, a heavy metal bioaccumulator cordgrass growing in the estuaries of Tinto, Odiel and Piedras River (south west Spain, one of the most polluted areas in the world. Strains were identified and ability to tolerate salt and heavy metals along with plant growth promoting and enzymatic properties were analysed. A high proportion of these bacteria were resistant towards one or several heavy metals and metalloids including As, Cu and Zn, the most abundant in plant tissues and soil. These strains also exhibited multiple enzymatic properties as amylase, cellulase, chitinase, protease and lipase, as well as plant growth promoting properties, including nitrogen fixation, phosphates solubilisation and production of indole-3-acetic acid (IAA, siderophores and 1-aminocyclopropane-1-carboxylate (ACC deaminase. The best performing strains (Micrococcus yunnanensis SMJ12, Vibrio sagamiensis SMJ18 and Salinicola peritrichatus SMJ30 were selected and tested as a consortium by inoculating S. maritima wild plantlets in greenhouse conditions along with wild polluted soil. After 30 days, bacterial inoculation improved plant photosynthetic traits and favoured intrinsic water use efficiency. However, far from stimulating plant metal uptake, endophytic inoculation lessened metal accumulation in above and belowground tissues. These results suggest that inoculation of S. maritima with indigenous metal-resistant endophytes could mean a useful approach in order to accelerate both adaption and growth of this indigenous cordgrass in polluted estuaries in restorative operations, but may not be suitable for rhizoaccumulation purposes.

  13. Spatial and temporal variations in aboveground and belowground biomass of Spartina maritima (small cordgrass) in created and natural marshes

    Science.gov (United States)

    Castillo, Jesús M.; Leira-Doce, Pablo; Rubio-Casal, Alfredo E.; Figueroa, Enrique

    2008-07-01

    Spartina species are commonly used for salt marsh manipulative projects, where aboveground and belowground biomasses are functional traits that play important roles, showing high spatial and temporal variations. This work analyses variations in AGB and BGB of Spartina maritima and abiotic environmental parameters along a chronosequence of six marshes created from 1997 to 2003 with disparate sediment dynamics, and adjacent natural marshes and unvegetated tidal flats. S. maritima behaved as an autogenic engineer, as its colonization of bare sediments yielded abiotic environmental changes: specifically, bed level rise accompanied by higher oxygenation and salinity. These modifications of the environment were site-specific, depending mainly on sedimentary dynamics. At the same time, abiotic environmental changes determined biomass production rates of S. maritima that were higher in more-accreting marshes; however, AGB was kept constant from early in its development (2 years). The increase in BGB with elevation seemed to be related to the inhibition of subsurface tissue development in anoxic sediments. Biomass accumulation and production varied markedly, depending on the spatial scale, indicating the relevance of the plot size chosen for the analysis of biomass of cordgrasses. Our results show that managers of salt marshes should consider sedimentary dynamics carefully when setting realistic expectations for success criteria of created and restored wetlands.

  14. Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana.

    Science.gov (United States)

    Hong, Young-Ho; Lee, Dong-Woo; Lee, Sang-Jae; Choe, Eun-Ah; Kim, Seong-Bo; Lee, Yoon-Hee; Cheigh, Chan-Ick; Pyun, Yu-Ryang

    2007-04-01

    Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t (1/2) = 43 days at 70 degrees C) in a continuous recycling mode at 70 degrees C produced 49 and 38 g D-tagatose/l from 180 and 90 g D-galactose/l, respectively, within 12 h.

  15. Kaempferol glycosides from Lobularia maritima and their potential role in plant interactions.

    Science.gov (United States)

    Fiorentino, Antonio; Ricci, Andreina; D'Abrosca, Brigida; Golino, Annunziata; Izzo, Angelina; Pascarella, Maria Teresa; Piccolella, Simona; Esposito, Assunta

    2009-02-01

    Six kaempferol glycosides, four of them characterized for the first time, were isolated from the leaf extract of Lobularia maritima. The structural elucidation was performed by a combined approach using Electrospray-Ionization Triple-Quadrupole Mass-Spectrometric (ESI/TQ/MS) techniques, and 1D- and 2D-NMR experiments (1H, 13C, DEPT, DQ-COSY, TOCSY, ROESY, NOESY, HSQC, HMBC, and HSQC-TOCSY). The isolated kaempferol derivatives have different disaccharide substituents at C(3) and four of them have a rhamnose unit at C(7). To evaluate their potential allelopathic role within the herbaceous plant community, the compounds, as well as the aglycone obtained from enzymatic hydrolysis, have been tested in vitro on three coexisting plant species, Dactylis hispanica, Petrorhagia velutina, and Phleum subulatum. The results obtained allow us to hypothesize that the type of the sugar modulates the biological response. The bioassay data, analyzed by a multivariate approach, and grouping the compounds on the basis of the number of sugar units and the nature of carbohydrates present in the disaccharide moiety, indicate a structure-activity relationship.

  16. Food and feeding ecology of purple sandpipers Calidris maritima on rocky intertidal habitats (Helgoland, German Bight)

    Science.gov (United States)

    Dierschke, Volker

    On the island of Helgoland (German Bight) Purple Sandpipers Calidris maritima feed mainly in the intertidal of piers and rocky shores. The main prey species are Littorina saxatilis and Mytilus edulis, complemented by crustaceans, polychaetes, other molluscs and green algae. Beach habitats are used as alternative feeding sites during storms. Feeding sites seem to be selected according to rates of assimilated energy intake. The most profitable habitat (wrack beds on the high-tide line with kelp-fly larvae, 16.8 W) is used after arrival in October but is not available during winter. Because of high intake rates in rocky habitats (13.1 W on piers, 5.5 W on mussel beds), which allow short daily feeding times, and available alternative feeding sites during storms, Purple Sandpipers do not need to carry fat reserves in winter like other waders wintering in central and Western Europe. This, and the ever accessible food supply of epibenthic macrofauna on rocky shores, may enable Purple Sandpipers to winter further north than other wader species.

  17. On the reproductive ecology of Suaeda maritima, S. monoica and S. nudiflora (Chenopodiaceae.

    Directory of Open Access Journals (Sweden)

    A.J. Solomon Raju

    2016-06-01

    Full Text Available Floral biology, sexual system, breeding system, pollinators, fruiting and seed dispersal aspects of three Suaeda species, S. maritima, S. monoica and S. nudiflora (Chenopodiaceae were studied.  The flowers of all the three species are hermaphroditic, dichogamous, strongly protogynous with a pistillage phase during the mature bud stage and staminate phase following anthesis, self-compatible exhibiting mixed breeding systems with special adaptation for cross-pollination; but both self- and cross-pollination are vector-dependent.  In all, the flowers display a mix of anemophilous and entomophilous traits.  Anemophily is effective in high salt marshes while water currents bring about pollination in low salt marshes; insects pollinate the flowers while collecting the forage from pistillate and staminate phase flowers. In these species, the whole plant breaks off and rolls on the floor while shedding its diaspores.  Fruits with seeds intact and/or seeds shed from fruits float on water due to their ability for buoyancy.  The fruits and seeds thus disperse, settle in the entire extent of salt marshes or coastal areas and germinate in mid-summer season when salinity is very high in high and low salt marshes.  

  18. XX/XY System of Sex Determination in the Geophilomorph Centipede Strigamia maritima.

    Directory of Open Access Journals (Sweden)

    Jack E Green

    Full Text Available We show that the geophilomorph centipede Strigamia maritima possesses an XX/XY system of sex chromosomes, with males being the heterogametic sex. This is, to our knowledge, the first report of sex chromosomes in any geophilomorph centipede. Using the recently assembled Strigamia genome sequence, we identified a set of scaffolds differentially represented in male and female DNA sequence. Using quantitative real-time PCR, we confirmed that three candidate X chromosome-derived scaffolds are present at approximately twice the copy number in females as in males. Furthermore, we confirmed that six candidate Y chromosome-derived scaffolds contain male-specific sequences. Finally, using this molecular information, we designed an X chromosome-specific DNA probe and performed fluorescent in situ hybridization against mitotic and meiotic chromosome spreads to identify the Strigamia XY sex-chromosome pair cytologically. We found that the X and Y chromosomes are recognizably different in size during the early pachytene stage of meiosis, and exhibit incomplete and delayed pairing.

  19. Is the reduced growth of the halophyte Suaeda maritima under hypoxia due to toxicity of iron or manganese?

    OpenAIRE

    Alhdad, Gazala M; Zörb, Christian; Al-Azzawi, Mohammed J; Flowers, Timothy J

    2015-01-01

    For most plants, submergence in water is a rare occurrence, but for plants that grow on salt marshes flooding with seawater may be a twice-daily event. This is the case for plants of the halophyte Suaeda maritima, growing at low elevations on salt marshes. These plants are, however, smaller than those growing at higher elevations, where flooding is less frequent and the soil is better drained. We investigated whether the reduced growth brought about by flooding with saline water was a consequ...

  20. EFFECT OF EXOGENOUS ABSCISIC ACID ON GROWTH AND BIOCHEMICAL CHANGES IN THE HALOPHYTE SUAEDA MARITIMA

    Directory of Open Access Journals (Sweden)

    Anbarasi G.

    2015-04-01

    Full Text Available Different types of phytohormones are being extensively used to alleviate the adverse effect of salinity stress on plant growth. Among those, Abscisic acid (ABA is a plant stress hormone and one of the most important signaling molecules in plants. Drought and salinity activate De-novo abscisic acid synthesis prevent further water loss by evaporation through stomata, mediated by changes in the guard cell turgor pressure. Under osmotic stress abscisic acid induce the accumulation of protein involved in the biosynthesis of osmolites which increasing the stress tolerance of plant. In addition, exogenous application of ABA enhances the tolerance of plants or plant cells to cold, heat, drought, anoxia and heavy metal stresses. This study was carried out to study the exogenous abscisic (ABA acid induced regulatory role on the growth, water content, protein content, chlorophyll content, osmolyte accumulation and protein profiling through SDS PAGE in a halophyte, Suaeda maritima. The osmolyte accumulation of proline and glycine betaine was found to be more in 50 µM ABA concentrations. The protein profiling through SDS PAGE revealed that ̴ 66KDa proteins was not expressed in the control plant and in 10μM ABA treated plants. Interestingly, the ABA treatment induced a new protein of 14.2KDa in 10μM concentration. The ABA treated plants with concentrations 50μM, 100μM and 150μM showed changes in the expression of protein in abundance than the control and 10μM ABA treated plants. The findings in this study indicate that among all the concentrations, 50μM ABA concentration treated plants exhibited higher growth rate.

  1. Biohydrogen production from untreated and hydrolyzed potato steam peels by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    Energy Technology Data Exchange (ETDEWEB)

    Mars, Astrid E.; Veuskens, Teun; Budde, Miriam A.W.; van Doeveren, Patrick F.N.M.; Lips, Steef J.; Bakker, Robert R.; de Vrije, Truus; Claassen, Pieternel A.M. [Wageningen UR, Food and Biobased Research, P.O. Box 17, 6700 AA Wageningen (Netherlands)

    2010-08-15

    Production of hydrogen by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana was studied in serum flasks and in pH-controlled bioreactors with glucose, and hydrolyzed and untreated potato steam peels (PSP) as carbon sources. Two types of PSP hydrolysates were used: one in which the starch in the PSP was liquefied with alpha-amylase, and one in which the liquefied starch was further hydrolyzed to glucose by amyloglucosidase. When the PSP hydrolysates or untreated PSP were added at circa 10-14 g/L of glucose units, both strains grew well and produced hydrogen with reasonable to high molar yields (2.4-3.8 moles H{sub 2}/mole glucose units), and no significant production of lactate. The hydrogen production rates and yields were similar with untreated PSP, hydrolyzed PSP, and pure glucose, showing that C. saccharolyticus and T. neapolitana are well equipped for the utilization of starch. When the concentrations of the substrates were increased, growth and hydrogen production of both strains were hampered. At substrate concentrations of circa 30-40 g/L of glucose units, the molar hydrogen yield of C. saccharolyticus was severely reduced due to the formation of high amounts of lactate, while T. neapolitana was unable to grow at all. The results showed that PSP and PSP hydrolysates are very suitable substrates for efficient fermentative hydrogen production at moderate substrate loadings. (author)

  2. β-Glucosidase from Thermotoga naphthophila RKU-10 for exclusive synthesis of galactotrisaccharides: Kinetics and thermodynamics insight into reaction mechanism.

    Science.gov (United States)

    Yang, Jingwen; Gao, Renjun; Zhou, Ye; Anankanbil, Sampson; Li, Jingbo; Xie, Guiqiu; Guo, Zheng

    2018-02-01

    This work reports a novel thermophilic β-glucosidase (TN0602) from Thermotoga naphthophila RKU-10, demonstrating exceptionally high catalytic selectivity (100%) for the exclusive synthesis of prebiotic galactotrisaccharides (GOS3) in a high volumetric production yield of 23.28gL -1 h -1 (higher than the highest value ever reported) at pH 6.5 and 75°C, with milk processing waste lactose as both the galactosyl donor and acceptor. A comparative study with commercial β-galactosidase from Aspergillus oryzae (AO) with respect to reaction kinetics, enzyme-substrate thermodynamic binding (substrate induced fluorescence quenching) and molecular docking simulation studies showed that β-glucosidase TN0602 has a deep catalytic "pocket" with a narrow entrance that prevents simultaneous access of lactose and GOS3 to the catalytic site, explaining its distinct catalytic specificity and reaction kinetics. The findings revealed in this work offer an improved understanding of how enzyme protein structure determines catalytic specificity, which serves as new knowledge to engineer β-glucosidase for the biosynthesis of designer GOS. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Synecology of Cutandia maritima (L. Barbey, a rare psammophytic species along the Montenegrin Coast (East Adriatic Coast

    Directory of Open Access Journals (Sweden)

    Stešević Danijela

    2017-12-01

    Full Text Available Cutandia maritima is a circum-Mediterranean species that inhabits sandy dunes along the coast line. It is fairly frequent on the western Adriatic coast but fairly rare and possibly even non-native in the east. In Croatia, it was discovered in 1990 in Crnika Bay on the island of Rab, which was considered until 2005 to be the only site on the eastern Adriatic coast from the Gulf of Trieste in the north to Corfu in the south. In 2009, the species was briefly reported for Velika plaža (Long Beach in Ulcinj (Montenegro but without details about the habitat type and synecology. The aim of this paper is thus to provide a deeper insight into the ecology and synecology of C. maritima in the eastern Adriatic part of the distribution area. On Velika plaža in Ulcinj, the species was found along the whole sea-inland gradient of sand dunes, in various types of vegetation: [1210] - annual vegetation of drift lines, [2110] - embryonic shifting dunes, [2120] - shifting dunes with Ammophila arenaria (white dunes, [2220] - dunes with Euphorbia terracina, [2130*] - fixed coastal dunes with herbaceous vegetation (grey dunes, and also [2190] - humid dune slacks.

  4. Effects of NaCl salinity on N-15-nitrate fluxes and specific root length in the halophyte Plantago maritima L.

    NARCIS (Netherlands)

    Rubinigg, M; Posthumus, F; Ferschke, M; Elzenga, JTM; Stulen, [No Value

    The effect of salinity on nitrate influx, efflux, nitrate net uptake rate and net nitrogen translocation to the shoot was assessed in a N-15 steady state labelling experiment in the halophyte Plantago maritima L. raised for 14 days on solution supplied with 50, 100 and 200 mol m(-3) sodium chloride

  5. Two psammophilic noctuids (Lepidoptera) newly associated with beach plum Prunus maritima: The Dune Noctuid (Sympistis riparia) and Coastal Heathland Cutworm (Abagrotis nefascia) in Northeastern North America

    Science.gov (United States)

    Beach Plum Prunus maritima Marshall 1785 not Wangenh. 1787 (Rosaceae) represents both a new crop under development and an under-acknowledged host plant for several Lepidoptera rthat have undergone declines in the Northeastern USA. The Coastal Heathland Cutworm Abagrotis nefascia (Smith) and the Dune...

  6. Obligate sugar oxidation in Mesotoga spp., phylum Thermotogae, in the presence of either elemental sulfur or hydrogenotrophic sulfate-reducers as electron acceptor

    OpenAIRE

    Fadhlaoui, K.; Ben Hania, W.; Armougom, Fabrice; Bartoli, M.; Fardeau, Marie-Laure; Erauso, G.; Brasseur, G.; Aubert, C.; Hamdi, M.; Brochier-Armanet, C.; Dolla, A.; Ollivier, Bernard

    2018-01-01

    Mesotoga prima strain PhosAc3 is a mesophilic representative of the phylum Thermotogae comprising only fermentative bacteria so far. We show that while unable to ferment glucose, this bacterium is able to couple its oxidation to reduction of elemental sulfur. We demonstrate furthermore that M. prima strain PhosAc3 as well as M. prima strain MesG1 and Mesotoga infera are able to grow in syntrophic association with sulfate-reducing bacteria (SRB) acting as hydrogen scavengers through interspeci...

  7. Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana

    Directory of Open Access Journals (Sweden)

    de Vrije Truus

    2009-06-01

    Full Text Available Abstract Background The production of hydrogen from biomass by fermentation is one of the routes that can contribute to a future sustainable hydrogen economy. Lignocellulosic biomass is an attractive feedstock because of its abundance, low production costs and high polysaccharide content. Results Batch cultures of Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana produced hydrogen, carbon dioxide and acetic acid as the main products from soluble saccharides in Miscanthus hydrolysate. The presence of fermentation inhibitors, such as furfural and 5-hydroxylmethyl furfural, in this lignocellulosic hydrolysate was avoided by the mild alkaline-pretreatment conditions at a low temperature of 75°C. Both microorganisms simultaneously and completely utilized all pentoses, hexoses and oligomeric saccharides up to a total concentration of 17 g l-1 in pH-controlled batch cultures. T. neapolitana showed a preference for glucose over xylose, which are the main sugars in the hydrolysate. Hydrogen yields of 2.9 to 3.4 mol H2 per mol of hexose, corresponding to 74 to 85% of the theoretical yield, were obtained in these batch fermentations. The yields were higher with cultures of C. saccharolyticus compared to T. neapolitana. In contrast, the rate of substrate consumption and hydrogen production was higher with T. neapolitana. At substrate concentrations exceeding 30 g l-1, sugar consumption was incomplete, and lower hydrogen yields of 2.0 to 2.4 mol per mol of consumed hexose were obtained. Conclusion Efficient hydrogen production in combination with simultaneous and complete utilization of all saccharides has been obtained during the growth of thermophilic bacteria on hydrolysate of the lignocellulosic feedstock Miscanthus. The use of thermophilic bacteria will therefore significantly contribute to the energy efficiency of a bioprocess for hydrogen production from biomass.

  8. Two rare halophyte species: Aster tripolium L. and Plantago maritima L. on the Baltic coast in Poland – their resources, distribution and implications for conservation management

    Directory of Open Access Journals (Sweden)

    Lazarus Magdalena

    2016-03-01

    Full Text Available The paper presents the results of geobotanical studies on the distribution and resources of Aster tripolium L. and Plantago maritima L, two rare halophytes in Poland. The research was conducted in northern Poland, along the Baltic coast in 2013. The present distribution of the two species was compared with historical data and general trends of and threats to these two species were examined. In total, 33 sites of A. tripolium and 18 of P. maritima were found in the research area. The resources of both species have been perceptibly depleting during last 150 years, which is mostly due to human agencies (e.g. habitat devastation caused by growing urban areas and the change in management and/or habitat condition. In order to preserve both species, it may be necessary to start an ex situ conservation program.

  9. Biotypes of scentless chamomile Matricaria maritima (L.) ssp. inodora (L.) Dostal and common poppy Papaver rhoeas (L.) resistant to tribenuron methyl, in Poland

    OpenAIRE

    Adamczewski Kazimierz; Kierzek Roman; Matysiak Kinga

    2014-01-01

    Scentless chamomile Matricaria maritima (L.) ssp. inodora (L.) Dostal and common poppy Papaver rhoeas (L.) are species which very often infest winter cereal and winter rape crops. Inhibitors of acetolactate synthase (ALS) are commonly used for control of these weeds. The herbicides are characterised by a single site of action in the plant, which has an influence on selection of the weed population and may result in a rapid development of resistance. In 2012, five seed samples of scen...

  10. Haplotype Detection from Next-Generation Sequencing in High-Ploidy-Level Species: 45S rDNA Gene Copies in the Hexaploid Spartina maritima

    Directory of Open Access Journals (Sweden)

    Julien Boutte

    2016-01-01

    Full Text Available Gene and whole-genome duplications are widespread in plant nuclear genomes, resulting in sequence heterogeneity. Identification of duplicated genes may be particularly challenging in highly redundant genomes, especially when there are no diploid parents as a reference. Here, we developed a pipeline to detect the different copies in the ribosomal RNA gene family in the hexaploid grass Spartina maritima from next-generation sequencing (Roche-454 reads. The heterogeneity of the different domains of the highly repeated 45S unit was explored by identifying single nucleotide polymorphisms (SNPs and assembling reads based on shared polymorphisms. SNPs were validated using comparisons with Illumina sequence data sets and by cloning and Sanger (resequencing. Using this approach, 29 validated polymorphisms and 11 validated haplotypes were reported (out of 34 and 20, respectively, that were initially predicted by our program. The rDNA domains of S. maritima have similar lengths as those found in other Poaceae, apart from the 5′-ETS, which is approximately two-times longer in S. maritima. Sequence homogeneity was encountered in coding regions and both internal transcribed spacers (ITS, whereas high intragenomic variability was detected in the intergenic spacer (IGS and the external transcribed spacer (ETS. Molecular cytogenetic analysis by fluorescent in situ hybridization (FISH revealed the presence of one pair of 45S rDNA signals on the chromosomes of S. maritima instead of three expected pairs for a hexaploid genome, indicating loss of duplicated homeologous loci through the diploidization process. The procedure developed here may be used at any ploidy level and using different sequencing technologies.

  11. Improved methane production from sugarcane vinasse with filter cake in thermophilic UASB reactors, with predominance of Methanothermobacter and Methanosarcina archaea and Thermotogae bacteria.

    Science.gov (United States)

    Barros, Valciney Gomes de; Duda, Rose Maria; Vantini, Juliana da Silva; Omori, Wellington Pine; Ferro, Maria Inês Tiraboschi; Oliveira, Roberto Alves de

    2017-11-01

    Biogas production from sugarcane vinasse has enormous economic, energy, and environmental management potential. However, methane production stability and biodigested vinasse quality remain key issues, requiring better nutrient and alkalinity availability, operational strategies, and knowledge of reactor microbiota. This study demonstrates increased methane production from vinasse through the use of sugarcane filter cake and improved effluent recirculation, with elevated organic loading rates (OLR) and good reactor stability. We used UASB reactors in a two-stage configuration, with OLRs up to 45gCODL -1 d -1 , and obtained methane production as high as 3LL -1 d -1 . Quantitative PCR indicated balanced amounts of bacteria and archaea in the sludge (10 9 -10 10 copiesg -1 VS), and of the predominant archaea orders, Methanobacteriales and Methanosarcinales (10 6 -10 8 copiesg -1 VS). 16S rDNA sequencing also indicated the thermophilic Thermotogae as the most abundant class of bacteria in the sludge. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense

    NARCIS (Netherlands)

    Kluskens, L.D.; Zeilstra, J.B.; Geerling, A.C.M.; Vos, de W.M.; Oost, van der J.

    2010-01-01

    The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized.

  13. Big wigs and small wigs: Time, sex, size and shelter affect cohabitation in the maritime earwig (Anisolabis maritima).

    Science.gov (United States)

    Hack, Nicole L; Iyengar, Vikram K

    2017-01-01

    Animal aggregations can occur for a variety of abiotic factors, such as resource limitation, or biotic factors, including group foraging and protection from predators. In our study, we examined whether time, sex, body size or shelter availability affected aggregation behavior of the maritime earwig, Anisolabis maritima (Order Dermaptera), an insect found globally at high densities under driftwood. Specifically, we monitored the distribution of two individuals in arenas with either two shelters (no habitat limitation) or one shelter (habitat limitation) to determine their propensity for cohabitation at times of peak activity and times of quiescence. Females, whose high levels of aggression are often associated with maternal care, were particularly averse to cohabitation, whereas males were generally more tolerant of other earwigs. Females initially preferred not to cohabitate when placed with a male, but were more tolerant of cohabitation later, regardless of the number of shelters. Same-sex pairs, on the other hand, were less likely to cohabitate with only one shelter present, but males were again more tolerant of conspecifics than females regardless of habitat limitation. When competition for one shelter did not lead to cohabitation, the lone occupant was more likely to be the larger individual in same-sex trials and females in mixed-sex trials. Understanding the tolerance for close proximity under these varying conditions may provide insight into aggregative behavior and spatial distribution patterns in the maritime earwig.

  14. The seasonal dormancy pattern and germination of Matricaria maritima subsp. inodora (L. Dostal seeds in hydrotime model terms

    Directory of Open Access Journals (Sweden)

    Anna Bochenek

    2011-01-01

    Full Text Available Changes in hydrotime model parameters were determined in Matricaria maritima L. subsp. inodora seeds during burial in a field in order to describe the seasonal dormancy pattern. Seeds were exhumed at regular intervals over a year and incubated at different water potentials at 19°C. Germination time courses were analyzed to determine hydrotime population parameters. Values of ѱb(50, ѲH and σѱb varied each month. Mean base water potential values in seeds exhumed each month were related to precipitation over 20 days before their exhumation. Soil temperature could be a trend-controlling factor of this relationship. The seeds were in deep dormancy after remaining 80-90 days in soil below or above limit temperature 15°C. The application of the hydrotime model to describe and predict seasonal dormancy patterns of weed seed is promising, especially for species with a considerable diversification of life strategies and ecophysiological flexibility of diaspores. It could also suggest mechanisms of seasonal dormancy changes of seeds in natural conditions and provide a basis for their examination. One of advantages of the dormancy pattern description of weed seeds remaining in a soil bank by means of threshold models is its simplicity.

  15. Big wigs and small wigs: Time, sex, size and shelter affect cohabitation in the maritime earwig (Anisolabis maritima.

    Directory of Open Access Journals (Sweden)

    Nicole L Hack

    Full Text Available Animal aggregations can occur for a variety of abiotic factors, such as resource limitation, or biotic factors, including group foraging and protection from predators. In our study, we examined whether time, sex, body size or shelter availability affected aggregation behavior of the maritime earwig, Anisolabis maritima (Order Dermaptera, an insect found globally at high densities under driftwood. Specifically, we monitored the distribution of two individuals in arenas with either two shelters (no habitat limitation or one shelter (habitat limitation to determine their propensity for cohabitation at times of peak activity and times of quiescence. Females, whose high levels of aggression are often associated with maternal care, were particularly averse to cohabitation, whereas males were generally more tolerant of other earwigs. Females initially preferred not to cohabitate when placed with a male, but were more tolerant of cohabitation later, regardless of the number of shelters. Same-sex pairs, on the other hand, were less likely to cohabitate with only one shelter present, but males were again more tolerant of conspecifics than females regardless of habitat limitation. When competition for one shelter did not lead to cohabitation, the lone occupant was more likely to be the larger individual in same-sex trials and females in mixed-sex trials. Understanding the tolerance for close proximity under these varying conditions may provide insight into aggregative behavior and spatial distribution patterns in the maritime earwig.

  16. Distributed Problem-Solving

    DEFF Research Database (Denmark)

    Chemi, Tatiana

    2016-01-01

    a perspective that is relevant to higher education. The focus here is on how artists solve problems in distributed paths, and on the elements of creative collaboration. Creative problem-solving will be looked at as an ongoing dialogue that artists engage with themselves, with others, with recipients......, what can educators at higher education learn from the ways creative groups solve problems? How can artists contribute to inspiring higher education?...

  17. Differences between the rhizosphere microbiome of Beta vulgaris ssp. maritima - ancestor of all beet crops - and modern sugar beets

    Directory of Open Access Journals (Sweden)

    Christin eZachow

    2014-08-01

    Full Text Available The structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. Effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. Therefore, we studied bacterial rhizosphere communities associated with the wild beet B. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (CS and modern sugar beets (Beta vulgaris ssp. vulgaris cultivated in CS and potting soil (PS under greenhouse conditions. Analysis of 16S rRNA gene fingerprints and pyrosequencing-based amplicon libraries revealed plant genotype- and soil-specific microbiomes. Wild beet plants harbor distinct operational taxonomic units (OTUs and a more diverse bacterial community than the domesticated sugar beet plants. Although the rhizospheres of both plant genotypes were dominated by Proteobacteria and Planctomycetes, 47.4% of dominant OTUs were additionally detected in the wild beet rhizosphere. Analysis of the cultivable fraction confirmed these plant genotype-specific differences at functional level. The proportion of isolates displayed in vitro activity against phytopathogens was lower for wild beet (≤45.8% than for sugar beet (≤57.5%. Conversely, active isolates from the wild beet exhibited stronger ability to cope with abiotic stresses. From all samples, active isolates of Stenotrophomonas rhizophila were frequently identified. In addition, soil type-specific impacts on the composition of bacterial communities were found: Acidobacteria, Chloroflexi, and Planctomycetes were only detected in plants cultivated in CS; whereas Bacteroidetes and Proteobacteria dominated in PS. Overall, in comparison to modern sugar beets, wild beets were associated with taxonomically and functionally distinct microbiomes.

  18. Problem Solving and Learning

    Science.gov (United States)

    Singh, Chandralekha

    2009-07-01

    One finding of cognitive research is that people do not automatically acquire usable knowledge by spending lots of time on task. Because students' knowledge hierarchy is more fragmented, "knowledge chunks" are smaller than those of experts. The limited capacity of short term memory makes the cognitive load high during problem solving tasks, leaving few cognitive resources available for meta-cognition. The abstract nature of the laws of physics and the chain of reasoning required to draw meaningful inferences makes these issues critical. In order to help students, it is crucial to consider the difficulty of a problem from the perspective of students. We are developing and evaluating interactive problem-solving tutorials to help students in the introductory physics courses learn effective problem-solving strategies while solidifying physics concepts. The self-paced tutorials can provide guidance and support for a variety of problem solving techniques, and opportunity for knowledge and skill acquisition.

  19. Creativity and Problem Solving

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    2004-01-01

    This paper presents some modern and interdisciplinary concepts about creativity and creative processes of special relevance for Operational Research workers. Central publications in the area Creativity-Operational Research are shortly reviewed. Some creative tools and the Creative Problem Solving...

  20. Teaching Creative Problem Solving.

    Science.gov (United States)

    Christensen, Kip W.; Martin, Loren

    1992-01-01

    Interpersonal and cognitive skills, adaptability, and critical thinking can be developed through problem solving and cooperative learning in technology education. These skills have been identified as significant needs of the workplace as well as for functioning in society. (SK)

  1. Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C-ring assembly in T3SS.

    Science.gov (United States)

    McDowell, Melanie A; Marcoux, Julien; McVicker, Gareth; Johnson, Steven; Fong, Yu Hang; Stevens, Rebecca; Bowman, Lesley A H; Degiacomi, Matteo T; Yan, Jun; Wise, Adam; Friede, Miriam E; Benesch, Justin L P; Deane, Janet E; Tang, Christoph M; Robinson, Carol V; Lea, Susan M

    2016-02-01

    Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) and a short variant (Spa33-C), with both required for secretion. They associate in a 1:2 complex (Spa33-FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL reveal a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM-FliN fusion and free FliN respectively. Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex structurally equivalent to Spa33-FL/C2 , allowing us to propose a unified model for C-ring assembly by NF-T3SS and flagellar-T3SS. © 2015 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

  2. Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-α-l-arabinanase from hyperthermophilic Thermotoga petrophila

    International Nuclear Information System (INIS)

    Squina, Fabio Marcio; Prade, Rolf Alexander; Wang, Hongliang; Murakami, Mario Tyago

    2009-01-01

    The crystallization and preliminary X-ray diffraction studies of an endo-1,5-α-arabinanase from hyperthermophilic T. petrophila are reported. The crystals diffracted to 2.86 Å resolution. The endo-1,5-α-l-arabinanases belonging to glycoside hydrolase family 43 are of great industrial interest for use in food technology, organic synthesis and biofuel production owing to their ability to catalyze the hydrolysis of α-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides. In this work, Thermotoga petrophila endo-1,5-α-l-arabinanase, a GH43-family member, has been cloned, overexpressed, purified and crystallized. Single crystals were obtained from a solution containing 0.1 M MES buffer pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 Ml-proline and 5%(v/v) dioxane. X-ray diffraction data were collected to a resolution of 2.86 Å using synchrotron radiation and the diffraction pattern was indexed in the tetragonal space group P422, with unit-cell parameters a = b = 83.71, c = 408.25 Å

  3. Genetic Diversity and Physiological Performance of Portuguese Wild Beet (Beta vulgaris spp. maritima) from Three Contrasting Habitats.

    Science.gov (United States)

    Ribeiro, Isa C; Pinheiro, Carla; Ribeiro, Carla M; Veloso, Maria M; Simoes-Costa, Maria C; Evaristo, Isabel; Paulo, Octávio S; Ricardo, Cândido P

    2016-01-01

    The establishment of stress resilient sugar beets (Beta vulgaris spp. vulgaris) is an important breeding goal since this cash crop is susceptible to drought and salinity. The genetic diversity in cultivated sugar beets is low and the beet wild relatives are useful genetic resources for tolerance traits. Three wild beet populations (Beta vulgaris spp. maritima) from contrasting environments, Vaiamonte (VMT, dry inland hill), Comporta (CMP, marsh) and Oeiras (OEI, coastland), and one commercial sugar beet (Isella variety, SB), are compared. At the genetic level, the use of six microsatellite allowed to detect a total of seventy six alleles. It was observed that CMP population has the highest value concerning the effective number of alleles and of expected heterozygosity. By contrast, sugar beet has the lowest values for all the parameters considered. Loci analysis with STRUCTURE allows defining three genetic clusters, the sea beet (OEI and CMP), the inland ruderal beet (VMT) and the sugar beet (SB). A screening test for progressive drought and salinity effects demonstrated that: all populations were able to recover from severe stress; drought impact was higher than that from salinity; the impact on biomass (total, shoot, root) was population specific. The distinct strategies were also visible at physiological level. We evaluated the physiological responses of the populations under drought and salt stress, namely at initial stress stages, late stress stages, and early stress recovery. Multivariate analysis showed that the physiological performance can be used to discriminate between genotypes, with a strong contribution of leaf temperature and leaf osmotic adjustment. However, the separation achieved and the groups formed are dependent on the stress type, stress intensity and duration. Each of the wild beet populations evaluated is very rich in genetic terms (allelic richness) and exhibited physiological plasticity, i.e., the capacity to physiologically adjust to

  4. Genetic diversity and physiological performance of Portuguese wild beet (Beta vulgaris spp. maritima from three contrasting habitats

    Directory of Open Access Journals (Sweden)

    Isa Catarina Ribeiro

    2016-08-01

    Full Text Available The establishment of stress resilient sugar beets (Beta vulgaris spp. vulgaris is an important breeding goal since this cash crop is susceptible to drought and salinity. The genetic diversity in cultivated sugar beets is low and the beet wild relatives are useful genetic resources for tolerance traits. Three wild beet populations (Beta vulgaris spp. maritima from contrasting environments, Vaiamonte (VMT, dry inland hill, Comporta (CMP, marsh and Oeiras (OEI, coastland, and one commercial sugar beet (Isella variety, SB, are compared. At the genetic level, the use of six microsatellite allowed to detect a total of seventy six alleles. It was observed that CMP population has the highest value concerning the effective number of alleles and of expected heterozygosity. By contrast, sugar beet has the lowest values for all the parameters considered. Loci analysis with STRUCTURE allows defining three genetic clusters, the sea beet (OEI and CMP, the inland ruderal beet (VMT and the sugar beet (SB. A screening test for progressive drought and salinity effects demonstrated that: all populations were able to recover from severe stress; drought impact was higher than that from salinity; the impact on biomass (total, shoot, root was population specific. The distinct strategies were also visible at physiological level. We evaluated the physiological responses of the populations under drought and salt stress, namely at initial stress stages, late stress stages and early stress recovery. Multivariate analysis showed that the physiological performance can be used to discriminate between genotypes, with a strong contribution of leaf temperature and leaf osmotic adjustment. However, the separation achieved and the groups formed are dependent on the stress type, stress intensity and duration. Each of the wild beet populations evaluated is very rich in genetic terms (allelic richness and exhibited physiological plasticity, i.e. the capacity to physiologically adjust

  5. Interaction indole-3-acetic acid IAA with lectin Canavalia maritima seeds reveal new function of lectins in plant physiology

    Energy Technology Data Exchange (ETDEWEB)

    Silva Filho, J.C.; Santi-Gadelha, T.; Gadelha, C.A.A.; Delatorre, P. [Universidade Federal da Paraiba (UFPB), Joao Pessoa, PB (Brazil); Teixeira, C.S.; Rocha, B.A.M.; Nobrega, R.B.; Alencar, K.L.L.; Cavada, B.S. [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil)

    2012-07-01

    Full text: Lectins are a class of proteins of non-immune origin characterized by its capability in interacts specifically and reversibly to mono and oligosaccharides. In plant several possible roles have been suggested including their function in seed maturation, cell wall assembly, defense mechanisms, or rhizobial nodulation of legume roots. Nearly all application and proposed of the plant lectins are based on their specific carbohydrate binding. However, it has been reported that lectins from legumes, might interact with other molecules, such as non proteic amino acids and hydrophobic compounds. This study show the first the crystal structure based on molecular replacement of the Canavalia maritima (CML) complexed with IAA correlated with possible role in plant development. Purified CML was dissolved in 20 mMTrisHCl pH 7.6 containing 5 mM IAA, the suitable co-crystals from CML-IAA complex grew in condition 4 of screen I (0.1 M TrisHCl pH 8.5 and 2.0 M ammonium sulfate). This crystal belong to the orthorhombic space group I222 with unit-cell parameters a = 67.1 ; b = 70.7 , c = 97.7 , The structure was refined at 2.1 of resolution to a final R factor of 20.63 % and an R free of 22.54 %. To check the relative position of the IAA molecule in relation to the biological assemble of the CML, the tetrameric structure was generate by crystallographic symmetry. IAA molecules are positioned in the central cavity. The IAA is stabilized by interacting through hydrogen bounds and Van der Waals forces with the amino acids residues Ser 108 and Asn131, and two water molecules. The hydrophilic interactions occur between IAA and side chains of Ser 108, Asn131 and water molecules 26 and 31 by H-bonds. The OG oxygen from Ser108 display H-bonds with O2 and O3 oxygen atoms from IAA, 3.1 and 2.8 respectively. The tetrameric structure of CML complexed with IAA revels which this protein can act during the seedling in plant development. (author)

  6. Adaptive Problem Solving

    Science.gov (United States)

    2017-03-01

    1) problem solving architecture; (2) problem representation ; (3) heuristics and control knowledge ; and (4) algorithms. In the area of problem... language which humans use to describe problems/domains and SAS+ is a standard problem representation input language used by many planners. Given a...implemented a system, MSP, that given a specific problem, automatically generates, evaluates, and assembles different combinations of representations and

  7. Electric Current Solves Mazes

    Science.gov (United States)

    Ayrinhac, Simon

    2014-01-01

    We present in this work a demonstration of the maze-solving problem with electricity. Electric current flowing in a maze as a printed circuit produces Joule heating and the right way is instantaneously revealed with infrared thermal imaging. The basic properties of electric current can be discussed in this context, with this challenging question:…

  8. Solving a binary puzzle

    NARCIS (Netherlands)

    P.H. Utomo (Putranto); R.H. Makarim (Rusydi)

    2017-01-01

    textabstractA Binary puzzle is a Sudoku-like puzzle with values in each cell taken from the set (Formula presented.). Let (Formula presented.) be an even integer, a solved binary puzzle is an (Formula presented.) binary array that satisfies the following conditions: (1) no three consecutive ones and

  9. On Solving Linear Recurrences

    Science.gov (United States)

    Dobbs, David E.

    2013-01-01

    A direct method is given for solving first-order linear recurrences with constant coefficients. The limiting value of that solution is studied as "n to infinity." This classroom note could serve as enrichment material for the typical introductory course on discrete mathematics that follows a calculus course.

  10. Solving Problems through Circles

    Science.gov (United States)

    Grahamslaw, Laura; Henson, Lisa H.

    2015-01-01

    Several problem-solving interventions that utilise a "circle" approach have been applied within the field of educational psychology, for example, Circle Time, Circle of Friends, Sharing Circles, Circle of Adults and Solution Circles. This research explored two interventions, Solution Circles and Circle of Adults, and used thematic…

  11. Solving Environmental Problems

    DEFF Research Database (Denmark)

    Ørding Olsen, Anders; Sofka, Wolfgang; Grimpe, Christoph

    2017-01-01

    dispersed. Hence, firms need to collaborate. We shed new light on collaborative search strategies led by firms in general and for solving environmental problems in particular. Both topics are largely absent in the extant open innovation literature. Using data from the European Seventh Framework Program...... for Research and Technological Development (FP7), our results indicate that the problem-solving potential of a search strategy increases with the diversity of existing knowledge of the partners in a consortium and with the experience of the partners involved. Moreover, we identify a substantial negative effect......Recent innovation and strategy research emphasizes the importance of firm’s search for external knowledge to improve innovation performance. We focus on such search strategies within the domain of sustainable innovation in which problems are inherently complex and the relevant knowledge is widely...

  12. Appreciative Problem Solving

    DEFF Research Database (Denmark)

    Hansen, David

    2012-01-01

    Many industrial production work systems have increased in complexity, and their new business model scompete on innovation, rather than low cost.At a medical device production facility committed to Lean Production, a research project was carried out to use Appreciative Inquiry to better engage...... employee strengths in continuou simprovements of the work system. The research question was: “How can Lean problem solving and Appreciative Inquiry be combined for optimized work system innovation?” The research project was carried out as a co-creation process with close cooperation between researcher...... to work system innovation and discusses how Appreciative Inquiry, Problem Solving, and the combination ‘Appreciative Problem Solving’ can be used to optimize continuous work system innovation.These findings add to the theoretical foundation of the emerging field of Strength-based Lean....

  13. Simon on problem solving

    DEFF Research Database (Denmark)

    Foss, Kirsten; Foss, Nicolai Juul

    2006-01-01

    as a general approach to problem solving. We apply these Simonian ideas to organisational issues, specifically new organisational forms. Specifically, Simonian ideas allow us to develop a morphology of new organisational forms and to point to some design problems that characterise these forms.......Two of Herbert Simon's best-known papers are 'The Architecture of Complexity' and 'The Structure of Ill-Structured Problems.' We discuss the neglected links between these two papers, highlighting the role of decomposition in the context of problems on which constraints have been imposed...

  14. Solving Differential Equations in R: Package deSolve

    NARCIS (Netherlands)

    Soetaert, K.E.R.; Petzoldt, T.; Setzer, R.W.

    2010-01-01

    In this paper we present the R package deSolve to solve initial value problems (IVP) written as ordinary differential equations (ODE), differential algebraic equations (DAE) of index 0 or 1 and partial differential equations (PDE), the latter solved using the method of lines approach. The

  15. Solved problems in electromagnetics

    CERN Document Server

    Salazar Bloise, Félix; Bayón Rojo, Ana; Gascón Latasa, Francisco

    2017-01-01

    This book presents the fundamental concepts of electromagnetism through problems with a brief theoretical introduction at the beginning of each chapter. The present book has a strong  didactic character. It explains all the mathematical steps and the theoretical concepts connected with the development of the problem. It guides the reader to understand the employed procedures to learn to solve the exercises independently. The exercises are structured in a similar way: The chapters begin with easy problems increasing progressively in the level of difficulty. This book is written for students of physics and engineering in the framework of the new European Plans of Study for Bachelor and Master and also for tutors and lecturers. .

  16. Solved problems in electrochemistry

    International Nuclear Information System (INIS)

    Piron, D.L.

    2004-01-01

    This book presents calculated solutions to problems in fundamental and applied electrochemistry. It uses industrial data to illustrate scientific concepts and scientific knowledge to solve practical problems. It is subdivided into three parts. The first uses modern basic concepts, the second studies the scientific basis for electrode and electrolyte thermodynamics (including E-pH diagrams and the minimum energy involved in transformations) and the kinetics of rate processes (including the energy lost in heat and in parasite reactions). The third part treats larger problems in electrolysis and power generation, as well as in corrosion and its prevention. Each chapter includes three sections: the presentation of useful principles; some twenty problems with their solutions; and, a set of unsolved problems

  17. A ocorrência do mutualismo facultativo entre Dyckia maritima Backer (Bromeliaceae e o cupim Cortaritermes silvestrii (Holmgren, Nasutitermitinae, em afloramentos rochosos no Parque Estadual de Itapuã, Viamão, RS

    Directory of Open Access Journals (Sweden)

    Waldemar Celso Copstein

    2003-01-01

    Full Text Available A presença de colônias de C. silvestrii é comum nos lajeados existentes em Itapuã. Na estação Morro da Grota1, 92,0 % dos termiteiros situados na rocha exposta e em ilhas de vegetação estão associados a D. maritima. Esta convivência ocorre em 31,2 % das ilhas na qual esta bromélia se faz presente. Nas ilhas, a comparação entre os substratos aonde D. maritima vegeta, o solo litólico húmico existente sob o manto do musgo Campylopus spp. e o substrato constituído pelo cupinzeiro indica que este último possui os teores mais elevados dos nutrientes P, K, Ca, Mg, Zn e Mn, maior CTC e maiores teores de partículas finas, principalmente o silte. O estabelecimento de D. maritima sobre os termiteiros de grande porte aumenta o seu valor de cobertura em ilhas de vegetação quando comparado com ilhas sem termiteiros ou com termiteiros de pequeno porte em áreas entre 2,7 a 8,0 m². Este fato é atribuído à melhoria físico-química do substrato e ao aumento de superfície e volume aptos a serem colonizados pela bromélia e proporciona maior competitividade em relação a outras espécies vegetais. As características apresentadas pela interação entre este cupim e D. maritima, pela primeira vez descrita na literatura, permitem indicar esta relação ecológica como mutualismo facultativo. Inferimos que o conjunto de observações apresentado constitui um modelo temporal de crescimento deste mutualismo, cujas fases inicial e tardia estão descritas neste trabalho.

  18. Computer Problem-Solving Coaches

    Science.gov (United States)

    Hsu, Leon; Heller, Kenneth

    2005-09-01

    Computers might be able to play an important role in physics instruction by coaching students to develop good problem-solving skills. Building on previous research on student problem solving and on designing computer programs to teach cognitive skills, we are developing a prototype computer coach to provide students with guided practice in solving problems. In addition to helping students become better problem solvers, such programs can be useful in studying how students learn to solve problems and how and if problem-solving skills can be transferred from a computer to a pencil-and-paper environment.

  19. Solving the Antibiotic Crisis.

    Science.gov (United States)

    Wright, Gerard D

    2015-02-13

    Antibiotics are essential for both treating and preventing infectious diseases. Paradoxically, despite their importance as pillars of modern medicine, we are in danger of losing antibiotics because of the evolution and dissemination of resistance mechanisms throughout all pathogenic microbes. This fact, coupled with an inability to bring new drugs to market at a pace that matches resistance, has resulted in a crisis of global proportion. Solving this crisis requires the actions of many stakeholders, but chemists, chemical biologists, and microbiologists must drive the scientific innovation that is required to maintain our antibiotic arsenal. This innovation requires (1) a deep understanding of the evolution and reservoirs of resistance; (2) full knowledge of the molecular mechanisms of antibiotic action and resistance; (3) the discovery of chemical and genetic probes of antibiotic action and resistance; (4) the integration of systems biology into antibiotic discovery; and (5) the discovery of new antimicrobial chemical matter. Addressing these pressing scientific gaps will ensure that we can meet the antibiotic crisis with creativity and purpose.

  20. Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization

    Directory of Open Access Journals (Sweden)

    Sahu Binod B

    2009-06-01

    Full Text Available Abstract Background Despite wealth of information generated on salt tolerance mechanism, its basics still remain elusive. Thus, there is a need of continued effort to understand the salt tolerance mechanism using suitable biotechnological techniques and test plants (species to enable development of salt tolerant cultivars of interest. Therefore, the present study was undertaken to generate information on salt stress responsive genes in a natural halophyte, Suaeda maritima, using PCR-based suppression subtractive hybridization (PCR-SSH technique. Results Forward and reverse SSH cDNA libraries were constructed after exposing the young plants to 425 mM NaCl for 24 h. From the forward SSH cDNA library, 429 high quality ESTs were obtained. BLASTX search and TIGR assembler programme revealed overexpression of 167 unigenes comprising 89 singletons and 78 contigs with ESTs redundancy of 81.8%. Among the unigenes, 32.5% were found to be of special interest, indicating novel function of these genes with regard to salt tolerance. Literature search for the known unigenes revealed that only 17 of them were salt-inducible. A comparative analysis of the existing SSH cDNA libraries for NaCl stress in plants showed that only a few overexpressing unigenes were common in them. Moreover, the present study also showed increased expression of phosphoethanolamine N-methyltransferase gene, indicating the possible accumulation of a much studied osmoticum, glycinebetaine, in halophyte under salt stress. Functional categorization of the proteins as per the Munich database in general revealed that salt tolerance could be largely determined by the proteins involved in transcription, signal transduction, protein activity regulation and cell differentiation and organogenesis. Conclusion The study provided a clear indication of possible vital role of glycinebetaine in the salt tolerance process in S. maritima. However, the salt-induced expression of a large number of genes

  1. Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization.

    Science.gov (United States)

    Sahu, Binod B; Shaw, Birendra P

    2009-06-05

    Despite wealth of information generated on salt tolerance mechanism, its basics still remain elusive. Thus, there is a need of continued effort to understand the salt tolerance mechanism using suitable biotechnological techniques and test plants (species) to enable development of salt tolerant cultivars of interest. Therefore, the present study was undertaken to generate information on salt stress responsive genes in a natural halophyte, Suaeda maritima, using PCR-based suppression subtractive hybridization (PCR-SSH) technique. Forward and reverse SSH cDNA libraries were constructed after exposing the young plants to 425 mM NaCl for 24 h. From the forward SSH cDNA library, 429 high quality ESTs were obtained. BLASTX search and TIGR assembler programme revealed overexpression of 167 unigenes comprising 89 singletons and 78 contigs with ESTs redundancy of 81.8%. Among the unigenes, 32.5% were found to be of special interest, indicating novel function of these genes with regard to salt tolerance. Literature search for the known unigenes revealed that only 17 of them were salt-inducible. A comparative analysis of the existing SSH cDNA libraries for NaCl stress in plants showed that only a few overexpressing unigenes were common in them. Moreover, the present study also showed increased expression of phosphoethanolamine N-methyltransferase gene, indicating the possible accumulation of a much studied osmoticum, glycinebetaine, in halophyte under salt stress. Functional categorization of the proteins as per the Munich database in general revealed that salt tolerance could be largely determined by the proteins involved in transcription, signal transduction, protein activity regulation and cell differentiation and organogenesis. The study provided a clear indication of possible vital role of glycinebetaine in the salt tolerance process in S. maritima. However, the salt-induced expression of a large number of genes involved in a wide range of cellular functions was

  2. Solving Sudoku with Constraint Programming

    Science.gov (United States)

    Crawford, Broderick; Castro, Carlos; Monfroy, Eric

    Constraint Programming (CP) is a powerful paradigm for modeling and solving Complex Combinatorial Problems (generally issued from Decision Making). In this work, we model the known Sudoku puzzle as a Constraint Satisfaction Problems and solve it with CP comparing the performance of different Variable and Value Selection Heuristics in its Enumeration phase. We encourage this kind of benchmark problem because it may suggest new techniques in constraint modeling and solving of complex systems, or aid the understanding of its main advantages and limits.

  3. Spatial analysis of nuclear and cytoplasmic DNA diversity in wild sea beet (Beta vulgaris ssp. maritima) populations: do marine currents shape the genetic structure?

    Science.gov (United States)

    Fievet, Virgil; Touzet, Pascal; Arnaud, Jean-François; Cuguen, Joël

    2007-05-01

    Patterns of seed dispersal in the wild sea beet (Beta vulgaris ssp. maritima) are predicted to be influenced by marine currents because populations are widely distributed along the European Atlantic coast. We investigated the potential influence of marine currents on the pattern of spatial genetic structuring in natural populations of sea beet. Populations were located along the French coasts of the Anglo-Norman gulf that features peculiar marine currents in the Channel. Thirty-three populations were sampled, among which 23 were continental and 10 were insular populations located in Jersey, Guernsey and Chausey, for a total of 1224 plants genotyped. To validate the coastal topography influence and the possibility of marine current orientated gene flow on the genetic features of sea beet populations, we assessed patterns of genetic structuring of cytoplasmic and nuclear diversity by: (i) searching for an isolation-by-distance (IBD) pattern using spatial autocorrelation tools; (ii) using the Monmonier algorithm to identify genetic boundaries in the area studied; and (iii) performing assignment tests that are based on multilocus genotype information to ascertain population membership of individuals. Our results showed a highly contrasted cytoplasmic and nuclear genetic differentiation and highlighted the peculiar situation of island populations. Beyond a classical isolation-by-distance due to short-range dispersal, genetic barriers fitting the orientation of marine currents were clearly identified. This suggests the occurrence of long-distance seed dispersal events and an asymmetrical gene flow separating the eastern and western part of the Anglo-Norman gulf.

  4. Subcellular concentrations of sugar alcohols and sugars in relation to phloem translocation in Plantago major, Plantago maritima, Prunus persica, and Apium graveolens.

    Science.gov (United States)

    Nadwodnik, Jan; Lohaus, Gertrud

    2008-04-01

    Sugar and sugar alcohol concentrations were analyzed in subcellular compartments of mesophyll cells, in the apoplast, and in the phloem sap of leaves of Plantago major (common plantain), Plantago maritima (sea plantain), Prunus persica (peach) and Apium graveolens (celery). In addition to sucrose, common plantain, sea plantain, and peach also translocated substantial amounts of sorbitol, whereas celery translocated mannitol as well. Sucrose was always present in vacuole and cytosol of mesophyll cells, whereas sorbitol and mannitol were found in vacuole, stroma, and cytosol in all cases except for sea plantain. The concentration of sorbitol, mannitol and sucrose in phloem sap was 2- to 40-fold higher than that in the cytosol of mesophyll cells. Apoplastic carbohydrate concentrations in all species tested were in the low millimolar range versus high millimolar concentrations in symplastic compartments. Therefore, the concentration ratios between the apoplast and the phloem were very strong, ranging between 20- to 100-fold for sorbitol and mannitol, and between 200- and 2000-fold for sucrose. The woody species, peach, showed the smallest concentration ratios between the cytosol of mesophyll cells and the phloem as well as between the apoplast and the phloem, suggesting a mixture of apoplastic and symplastic phloem loading, in contrast to the herbal plant species (common plantain, sea plantain, celery) which likely exhibit an active loading mode for sorbitol and mannitol as well as sucrose from the apoplast into the phloem.

  5. Difficulties in Genetics Problem Solving.

    Science.gov (United States)

    Tolman, Richard R.

    1982-01-01

    Examined problem-solving strategies of 30 high school students as they solved genetics problems. Proposes a new sequence of teaching genetics based on results: meiosis, sex chromosomes, sex determination, sex-linked traits, monohybrid and dihybrid crosses (humans), codominance (humans), and Mendel's pea experiments. (JN)

  6. Problem Solving in the Professions.

    Science.gov (United States)

    Jackling, Noel; And Others

    1990-01-01

    It is proposed that algorithms and heuristics are useful in improving professional problem-solving abilities when contextualized within the academic discipline. A basic algorithm applied to problem solving in undergraduate engineering education and a similar algorithm applicable to legal problems are used as examples. Problem complexity and…

  7. Crime Solving Techniques: Training Bulletin.

    Science.gov (United States)

    Sands, Jack M.

    The document is a training bulletin for criminal investigators, explaining the use of probability, logic, lateral thinking, group problem solving, and psychological profiles as methods of solving crimes. One chpater of several pages is devoted to each of the five methods. The use of each method is explained; problems are presented for the user to…

  8. Combinatorial reasoning to solve problems

    NARCIS (Netherlands)

    Coenen, Tom Johannes Maria; Hof, Frits; Verhoef, Neeltje Cornelia

    2016-01-01

    This study reports combinatorial reasoning to solve problems. We observed the mathematical thinking of students aged 14-16. We study the variation of the students’ solution strategies in the context of emergent modelling. The results show that the students are tempted to begin the problem solving

  9. Solving complex fisheries management problems

    DEFF Research Database (Denmark)

    Petter Johnsen, Jahn; Eliasen, Søren Qvist

    2011-01-01

    A crucial issue for the new EU common fisheries policy is how to solve the discard problem. Through a study of the institutional set up and the arrangements for solving the discard problem in Denmark, the Faroe Islands, Iceland and Norway, the article identifies the discard problem as related to ...... to both natural, other material and to cultural conditions. Hence, solving the discard problem requires not only technical and regulatory instruments, but also arenas and structures that allow and facilitate processes of cultural change....

  10. Pulsating Star Mystery Solved

    Science.gov (United States)

    2010-11-01

    By discovering the first double star where a pulsating Cepheid variable and another star pass in front of one another, an international team of astronomers has solved a decades-old mystery. The rare alignment of the orbits of the two stars in the double star system has allowed a measurement of the Cepheid mass with unprecedented accuracy. Up to now astronomers had two incompatible theoretical predictions of Cepheid masses. The new result shows that the prediction from stellar pulsation theory is spot on, while the prediction from stellar evolution theory is at odds with the new observations. The new results, from a team led by Grzegorz Pietrzyński (Universidad de Concepción, Chile, Obserwatorium Astronomiczne Uniwersytetu Warszawskiego, Poland), appear in the 25 November 2010 edition of the journal Nature. Grzegorz Pietrzyński introduces this remarkable result: "By using the HARPS instrument on the 3.6-metre telescope at ESO's La Silla Observatory in Chile, along with other telescopes, we have measured the mass of a Cepheid with an accuracy far greater than any earlier estimates. This new result allows us to immediately see which of the two competing theories predicting the masses of Cepheids is correct." Classical Cepheid Variables, usually called just Cepheids, are unstable stars that are larger and much brighter than the Sun [1]. They expand and contract in a regular way, taking anything from a few days to months to complete the cycle. The time taken to brighten and grow fainter again is longer for stars that are more luminous and shorter for the dimmer ones. This remarkably precise relationship makes the study of Cepheids one of the most effective ways to measure the distances to nearby galaxies and from there to map out the scale of the whole Universe [2]. Unfortunately, despite their importance, Cepheids are not fully understood. Predictions of their masses derived from the theory of pulsating stars are 20-30% less than predictions from the theory of the

  11. How to solve mathematical problems

    CERN Document Server

    Wickelgren, Wayne A

    1995-01-01

    Seven problem-solving techniques include inference, classification of action sequences, subgoals, contradiction, working backward, relations between problems, and mathematical representation. Also, problems from mathematics, science, and engineering with complete solutions.

  12. Chemical Composition and Biological Studies of the Essential Oil from Aerial Parts of Beta vulgaris subsp. maritima (L.) Arcang. Growing in Tunisia.

    Science.gov (United States)

    Zardi-Bergaoui, Afifa; Ben Nejma, Aymen; Harzallah-Skhiri, Fethia; Flamini, Guido; Ascrizzi, Roberta; Ben Jannet, Hichem

    2017-10-01

    The chemical composition, antioxidant, cytotoxic, anticholinesterase and anti-tyrosinase activities of the hydrodistilled essential oil of the aerial parts of Beta vulgaris subsp. maritime (L.) Arcang. from Tunisia have been evaluated. The chemical composition of the oil (yield 0.037% [w/w]), determined by GC-FID and GC/MS is reported for the first time. Twenty five components, accounting for 98.1% of the total oil have been identified. The oil was characterized by a high proportion of oxygenated sesquiterpenes (39.2%), followed by sesquiterpene hydrocarbons (30.3%) and one apocarotenoids (26.3%). The main compounds were γ-irone (26.3%), α-cadinol (12.1%), T-cadinol (10.6%), bicyclogermacrene (10.4%) and δ-cadinene (6.0%). The isolated oil was tested for its antioxidant activity using the DPPH · , ABTS +· , catalase, and paraoxonase assays and also for its cytotoxic, anticholinesterase, and anti-tyrosinase activities. The essential oil exhibited high antioxidant activity (IC 50  = 0.055 ± 0.006 mg/ml) and important result oncatalase (524.447 ± 2.58 Units/mg protein). Furthermore, it exerted a significant cytotoxic effect against A549 cell line, with IC 50  = 42.44 ± 1.40 μg/ml. The results indicate that the essential oil of B. vulgaris subsp. maritima (L.) Arcang. aerial parts may be used in future as an alternative to synthetic antioxidant agents, with potential application in the food and pharmaceutical industries. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  13. A Population-Based Approach to Restore and Manage Ruppia Maritima (Wigeongrass) in the Highly Variable Everglades-Florida Bay Ecotone

    Science.gov (United States)

    Strazisar, T. M.; Koch, M.; Madden, C. J.

    2016-02-01

    Seagrasses and submerged aquatic vegetation (SAV) continue to decline globally from human-induced disturbance and habitat loss in estuarine and coastal ecosystems. The SAV Ruppia maritima historically created critical habitat at the Everglades-Florida Bay ecotone, but hydrological modifications and lower freshwater flows have resulted in significant declines in recent decades. We used a population-based approach to examine factors controlling Ruppia presence and abundance at the ecotone to expand the scientific base for management and restoration of SAV species in highly variable environments and examine factors required for Ruppia restoration in the Everglades. Life history transitions from seed through sexual reproduction were established under a range of field conditions critical to seagrass and SAV persistence, including salinity, temperature, light, sediment nutrients (P) and competitor SAV. We found multiple constraints to Ruppia life history development, including an ephemeral seed bank, low rates of successful germination and seedling survival and clonal reproduction limited by variable salinity, nutrients, light and competition with the macroalga Chara hornemannii. Because of low survival rates and limited clonal reproduction, Ruppia at the Evergaldes ecotone currently depends on high rates of viable seed production. However, development of large reproductive meadows requires high vegetative shoot densities. Thus, Everglades restoration should establish lower salinities to create higher seedling and adult survival and clonal reproduction to support successful sexual reproduction that can build up the seed bank for years when adult survival is limited. This population-based data from field experiments and surveys is being incorporated into a seagrass model to enable forecasting of population sustainability and evaluate Everglades restoration targets which includes restoring Ruppia to the southern Everglades-Florida Bay ecotone.

  14. Differences between the rhizosphere microbiome of Beta vulgaris ssp. maritima-ancestor of all beet crops-and modern sugar beets.

    Science.gov (United States)

    Zachow, Christin; Müller, Henry; Tilcher, Ralf; Berg, Gabriele

    2014-01-01

    The structure and function of the plant microbiome is driven by plant species and prevailing environmental conditions. Effectuated by breeding efforts, modern crops diverge genetically and phenotypically from their wild relatives but little is known about consequences for the associated microbiota. Therefore, we studied bacterial rhizosphere communities associated with the wild beet B. vulgaris ssp. maritima grown in their natural habitat soil from coastal drift lines (CS) and modern sugar beets (Beta vulgaris ssp. vulgaris) cultivated in CS and potting soil (PS) under greenhouse conditions. Analysis of 16S rRNA gene fingerprints and pyrosequencing-based amplicon libraries revealed plant genotype- and soil-specific microbiomes. Wild beet plants harbor distinct operational taxonomic units (OTUs) and a more diverse bacterial community than the domesticated sugar beet plants. Although the rhizospheres of both plant genotypes were dominated by Proteobacteria and Planctomycetes, 37.5% of dominant OTUs were additionally detected in the wild beet rhizosphere. Analysis of the cultivable fraction confirmed these plant genotype-specific differences at functional level. The proportion of isolates displayed in vitro activity against phytopathogens was lower for wild beet (≤45.8%) than for sugar beet (≤57.5%). Conversely, active isolates from the wild beet exhibited stronger ability to cope with abiotic stresses. From all samples, active isolates of Stenotrophomonas rhizophila were frequently identified. In addition, soil type-specific impacts on the composition of bacterial communities were found: Acidobacteria, Chloroflexi, and Planctomycetes were only detected in plants cultivated in CS; whereas Bacteroidetes and Proteobacteria dominated in PS. Overall, in comparison to modern sugar beets, wild beets were associated with taxonomically and functionally distinct microbiomes.

  15. Aging and skilled problem solving.

    Science.gov (United States)

    Charness, N

    1981-03-01

    Information-processing models of problem solving too often are based on restrictive age ranges. On the other hand, gerontologists have investigated few problem-solving tasks and have rarely generated explicit models. As this article demonstrates, both fields can benefit by closer collaboration. One major issue in gerontology is whether aging is associated with irreversible decrement or developmental plasticity. If both processes occur, then an appropriate strategy for investigating aging is to equate age groups for molar problem-solving performance and search for differences in the underlying components. This strategy was adopted to examine the relation of age and skill to problem solving in chess. Chess players were selected to vary widely in age and skill such that these variables were uncorrelated. Problem-solving and memory tasks were administered. Skill level was the only significant predictor for accuracy in both a choose-a-move task and a speeded end-game evaluation task. Age (negatively) and skill (positively) jointly determined performance in an unexpected recall task. Efficient chunking in recall was positively related to skill, though negatively related to age. Recognition confidence, though not accuracy, was negatively related to age. Thus despite age-related declines in encoding and retrieval of information, older players match the problem-solving performance of equivalently skilled younger players. Apparently, they can search the problem space more efficiently, as evidenced by taking less time to select an equally good move. Models of chess skill that stress that role of encoding efficiency, as indexed by chunking in recall, need to be modified to account for performance over the life span.

  16. Simon on Problem-Solving

    DEFF Research Database (Denmark)

    Foss, Kirsten; Foss, Nicolai Juul

    as a general approach to problem solving. We apply these Simonian ideas to organizational issues, specifically new organizational forms. Specifically, Simonian ideas allow us to develop a morphology of new organizational forms and to point to some design problems that characterize these forms.Keywords: Herbert...... Simon, problem-solving, new organizational forms. JEL Code: D23, D83......Two of Herbert Simon's best-known papers are "The Architecture of Complexity" and "The Structure of Ill-Structured Problems." We discuss the neglected links between these two papers, highlighting the role of decomposition in the context of problems on which constraints have been imposed...

  17. Interactive problem solving using LOGO

    CERN Document Server

    Boecker, Heinz-Dieter; Fischer, Gerhard

    2014-01-01

    This book is unique in that its stress is not on the mastery of a programming language, but on the importance and value of interactive problem solving. The authors focus on several specific interest worlds: mathematics, computer science, artificial intelligence, linguistics, and games; however, their approach can serve as a model that may be applied easily to other fields as well. Those who are interested in symbolic computing will find that Interactive Problem Solving Using LOGO provides a gentle introduction from which one may move on to other, more advanced computational frameworks or more

  18. Customer-centered problem solving.

    Science.gov (United States)

    Samelson, Q B

    1999-11-01

    If there is no single best way to attract new customers and retain current customers, there is surely an easy way to lose them: fail to solve the problems that arise in nearly every buyer-supplier relationship, or solve them in an unsatisfactory manner. Yet, all too frequently, companies do just that. Either we deny that a problem exists, we exert all our efforts to pin the blame elsewhere, or we "Band-Aid" the problem instead of fixing it, almost guaranteeing that we will face it again and again.

  19. Solved problems in classical electromagnetism

    CERN Document Server

    Franklin, Jerrold

    2018-01-01

    This original Dover publication is the companion to a new edition of the author's Classical Electromagnetism: Second Edition. The latter volume will feature only basic answers; this book will contain some problems from the reissue as well as many other new ones. All feature complete, worked-out solutions and form a valuable source of problem-solving material for students.

  20. Quantitative Reasoning in Problem Solving

    Science.gov (United States)

    Ramful, Ajay; Ho, Siew Yin

    2015-01-01

    In this article, Ajay Ramful and Siew Yin Ho explain the meaning of quantitative reasoning, describing how it is used in the to solve mathematical problems. They also describe a diagrammatic approach to represent relationships among quantities and provide examples of problems and their solutions.

  1. Interactive Problem-Solving Interventions

    African Journals Online (AJOL)

    Frew Demeke Alemu

    various interactive problem solving methods. Track III Diplomacy refers to “people to people” search for common ground undertaken by individuals or private groups. This type of activity may involve organizing meetings and conferences, generating media exposure, and political and legal advocacy. 15 Wehrenfennig, Id., p.

  2. Solving Differential Equations in R: Package deSolve

    Directory of Open Access Journals (Sweden)

    Karline Soetaert

    2010-02-01

    Full Text Available In this paper we present the R package deSolve to solve initial value problems (IVP written as ordinary differential equations (ODE, differential algebraic equations (DAE of index 0 or 1 and partial differential equations (PDE, the latter solved using the method of lines approach. The differential equations can be represented in R code or as compiled code. In the latter case, R is used as a tool to trigger the integration and post-process the results, which facilitates model development and application, whilst the compiled code significantly increases simulation speed. The methods implemented are efficient, robust, and well documented public-domain Fortran routines. They include four integrators from the ODEPACK package (LSODE, LSODES, LSODA, LSODAR, DVODE and DASPK2.0. In addition, a suite of Runge-Kutta integrators and special-purpose solvers to efficiently integrate 1-, 2- and 3-dimensional partial differential equations are available. The routines solve both stiff and non-stiff systems, and include many options, e.g., to deal in an efficient way with the sparsity of the Jacobian matrix, or finding the root of equations. In this article, our objectives are threefold: (1 to demonstrate the potential of using R for dynamic modeling, (2 to highlight typical uses of the different methods implemented and (3 to compare the performance of models specified in R code and in compiled code for a number of test cases. These comparisons demonstrate that, if the use of loops is avoided, R code can efficiently integrate problems comprising several thousands of state variables. Nevertheless, the same problem may be solved from 2 to more than 50 times faster by using compiled code compared to an implementation using only R code. Still, amongst the benefits of R are a more flexible and interactive implementation, better readability of the code, and access to R’s high-level procedures. deSolve is the successor of package odesolve which will be deprecated in

  3. Ligand binding and conformational dynamics in a flavin-based electron-bifurcating enzyme complex revealed by Hydrogen-Deuterium Exchange Mass Spectrometry.

    Science.gov (United States)

    Demmer, Julius K; Rupprecht, Fiona A; Eisinger, Martin L; Ermler, Ulrich; Langer, Julian D

    2016-12-01

    Flavin-based electron bifurcation (FBEB) is a novel mechanism of energy coupling used by anaerobic microorganisms to optimize their energy metabolism efficiency. The first high-resolution structure of a complete FBEB enzyme complex, the NADH-dependent reduced ferredoxin: NADP + -oxidoreductase (NfnAB) of Thermotoga maritima, was recently solved. However, no experimental evidence for the NADPH-binding site and conformational changes during the FBEB reaction are available. Here we analyzed ligand binding and the conformational dynamics of oxygen-sensitive NfnAB using Hydrogen-Deuterium Exchange Mass-Spectrometry, including a customized anaerobic workflow. We confirmed the NADH and the previously postulated NADPH-binding site. Furthermore, we observed an NfnA-NfnB rearrangement upon NADPH binding which supports the proposed FBEB mechanism. © 2016 Federation of European Biochemical Societies.

  4. Formation and subdivision of the head field in the centipede Strigamia maritima, as revealed by the expression of head gap gene orthologues and hedgehog dynamics

    Directory of Open Access Journals (Sweden)

    Vera S. Hunnekuhl

    2017-10-01

    Full Text Available Abstract Background There have been few studies of head patterning in non-insect arthropods, and even in the insects, much is not yet understood. In the fly Drosophila three head gap genes, orthodenticle (otd, buttonhead (btd and empty spiracles (ems are essential for patterning the head. However, they do not act through the same pair-rule genes that pattern the trunk from the mandibular segment backwards. Instead they act through the downstream factors collier (col and cap‘n’collar (cnc, and presumably other unknown factors. In the beetle Tribolium, these same gap and downstream genes are also expressed during early head development, but in more restricted domains, and some of them have been shown to be of minor functional importance. In the spider Parasteatoda tepidariorum, hedgehog (hh and otd have been shown to play an important role in head segmentation. Results We have investigated the expression dynamics of otx (otd, SP5/btd, ems, and the downstream factors col, cnc and hh during early head development of the centipede Strigamia maritima. Our results reveal the process of head condensation and show that the anteroposterior sequence of specific gene expression is conserved with that in insects. SP5/btd and otx genes are expressed prior to and during head field formation, whereas ems is not expressed until after the initial formation of the head field, in an emerging gap between SP5/btd and otx expression. Furthermore, we observe an early domain of Strigamia hh expression in the head field that splits to produce segmental stripes in the ocular, antennal and intercalary segments. Conclusions The dynamics of early gene expression in the centipede show considerable similarity with that in the beetle, both showing more localised expression of head gap genes than occurs in the fly. This suggests that the broad overlapping domains of head gap genes observed in Drosophila are derived in this lineage. We also suggest that the splitting of the

  5. Formation and subdivision of the head field in the centipede Strigamia maritima, as revealed by the expression of head gap gene orthologues and hedgehog dynamics.

    Science.gov (United States)

    Hunnekuhl, Vera S; Akam, Michael

    2017-01-01

    There have been few studies of head patterning in non-insect arthropods, and even in the insects, much is not yet understood. In the fly Drosophila three head gap genes, orthodenticle ( otd ), buttonhead ( btd ) and empty spiracles ( ems ) are essential for patterning the head. However, they do not act through the same pair-rule genes that pattern the trunk from the mandibular segment backwards. Instead they act through the downstream factors collier ( col ) and cap ' n ' collar ( cnc ), and presumably other unknown factors. In the beetle Tribolium , these same gap and downstream genes are also expressed during early head development, but in more restricted domains, and some of them have been shown to be of minor functional importance. In the spider Parasteatoda tepidariorum , hedgehog ( hh ) and otd have been shown to play an important role in head segmentation. We have investigated the expression dynamics of otx ( otd ), SP5 / btd , ems , and the downstream factors col , cnc and hh during early head development of the centipede Strigamia maritima . Our results reveal the process of head condensation and show that the anteroposterior sequence of specific gene expression is conserved with that in insects. SP5 / btd and otx genes are expressed prior to and during head field formation, whereas ems is not expressed until after the initial formation of the head field, in an emerging gap between SP5 / btd and otx expression. Furthermore, we observe an early domain of Strigamia hh expression in the head field that splits to produce segmental stripes in the ocular, antennal and intercalary segments. The dynamics of early gene expression in the centipede show considerable similarity with that in the beetle, both showing more localised expression of head gap genes than occurs in the fly. This suggests that the broad overlapping domains of head gap genes observed in Drosophila are derived in this lineage. We also suggest that the splitting of the early hh segmental stripes

  6. Assessing Algebraic Solving Ability: A Theoretical Framework

    Science.gov (United States)

    Lian, Lim Hooi; Yew, Wun Thiam

    2012-01-01

    Algebraic solving ability had been discussed by many educators and researchers. There exists no definite definition for algebraic solving ability as it can be viewed from different perspectives. In this paper, the nature of algebraic solving ability in terms of algebraic processes that demonstrate the ability in solving algebraic problem is…

  7. Assertiveness and problem solving in midwives

    Directory of Open Access Journals (Sweden)

    Zeliha Burcu Yurtsal

    2015-01-01

    Conclusions: There were significant statistical differences between assertiveness levels and problem solving skills of midwives, and midwives who were assertive solved their problems better than did others. Assertiveness and problem-solving skills training will contribute to the success of the midwifery profession. Midwives able to solve problems, and display assertive behaviors will contribute to the development of midwifery profession.

  8. An NOy* Algorithm for SOLVE

    Science.gov (United States)

    Loewenstein, M.; Greenblatt. B. J.; Jost, H.; Podolske, J. R.; Elkins, Jim; Hurst, Dale; Romanashkin, Pavel; Atlas, Elliott; Schauffler, Sue; Donnelly, Steve; hide

    2000-01-01

    De-nitrification and excess re-nitrification was widely observed by ER-2 instruments in the Arctic vortex during SOLVE in winter/spring 2000. Analyses of these events requires a knowledge of the initial or pre-vortex state of the sampled air masses. The canonical relationship of NOy to the long-lived tracer N2O observed in the unperturbed stratosphere is generally used for this purpose. In this paper we will attempt to establish the current unperturbed NOy:N2O relationship (NOy* algorithm) using the ensemble of extra-vortex data from in situ instruments flying on the ER-2 and DC-8, and from the Mark IV remote measurements on the OMS balloon. Initial analysis indicates a change in the SOLVE NOy* from the values predicted by the 1994 Northern Hemisphere NOy* algorithm which was derived from the observations in the ASHOE/MAESA campaign.

  9. Methods of solving nonstandard problems

    CERN Document Server

    Grigorieva, Ellina

    2015-01-01

    This book, written by an accomplished female mathematician, is the second to explore nonstandard mathematical problems – those that are not directly solved by standard mathematical methods but instead rely on insight and the synthesis of a variety of mathematical ideas.   It promotes mental activity as well as greater mathematical skills, and is an ideal resource for successful preparation for the mathematics Olympiad. Numerous strategies and techniques are presented that can be used to solve intriguing and challenging problems of the type often found in competitions.  The author uses a friendly, non-intimidating approach to emphasize connections between different fields of mathematics and often proposes several different ways to attack the same problem.  Topics covered include functions and their properties, polynomials, trigonometric and transcendental equations and inequalities, optimization, differential equations, nonlinear systems, and word problems.   Over 360 problems are included with hints, ...

  10. Problem solving through recreational mathematics

    CERN Document Server

    Averbach, Bonnie

    1999-01-01

    Historically, many of the most important mathematical concepts arose from problems that were recreational in origin. This book takes advantage of that fact, using recreational mathematics - problems, puzzles and games - to teach students how to think critically. Encouraging active participation rather than just observation, the book focuses less on mathematical results than on how these results can be applied to thinking about problems and solving them. Each chapter contains a diverse array of problems in such areas as logic, number and graph theory, two-player games of strategy, solitaire ga

  11. Solving rational expectations models using Excel

    DEFF Research Database (Denmark)

    Strulik, Holger

    2004-01-01

    Problems of discrete time optimal control can be solved using backward iteration and Microsoft Excel. The author explains the method in general and shows how the basic models of neoclassical growth and real business cycles are solved......Problems of discrete time optimal control can be solved using backward iteration and Microsoft Excel. The author explains the method in general and shows how the basic models of neoclassical growth and real business cycles are solved...

  12. Effects of practicing creative problem solving

    OpenAIRE

    Bojanović, Radojica; Đurišić-Bojanović, Mirosava

    2016-01-01

    This paper tests the effect of practicing creative problem solving in the context of business. The research was conducted on a sample of final-year students (N=90). Participants were solving the initial and final test of creative problem solving in the context of business, as well as the creativity test. Practicing creative problem solving lasted around four monts (around 40 minutes a week). Practice method combined a nominal method of group decision making and Osborn creativity method. The b...

  13. LEGO Robotics: An Authentic Problem Solving Tool?

    Science.gov (United States)

    Castledine, Alanah-Rei; Chalmers, Chris

    2011-01-01

    With the current curriculum focus on correlating classroom problem solving lessons to real-world contexts, are LEGO robotics an effective problem solving tool? This present study was designed to investigate this question and to ascertain what problem solving strategies primary students engaged with when working with LEGO robotics and whether the…

  14. Solving the structure of metakaolin

    Energy Technology Data Exchange (ETDEWEB)

    Proffen, Thomas E [Los Alamos National Laboratory; White, Claire E [Los Alamos National Laboratory; Provis, John L [UNIV. OF MELBOURNE; Riley, Daniel P [UNIV. OF MELBOURNE; Van Deventer, Jannie S J [UNIV. OF MELBOURNE

    2009-01-01

    Metakaolin has been used extensively as a cement additive and paint extender, and recently as a geopolymer precursor. This disordered layered aluminosilicate is formed via the dehydroxylation of kaolinite. However, an accurate representation of its atomic structure has bever before been presented. Here, a novel synergy between total scattering and density functional modeling is presented to solve the structure of metakaolin. The metastable structure is elucidated by iterating between least-squares real-space refinement using neutron pair distribution function data, and geometry optimization using density functional modeling. The resulting structural representation is both energetically feasible and in excellent agreement with experimental data. This accurate structure of metakaolin provides new insight into the local environment of the aluminum atoms, with evidence of the existence of tri-coordinated aluminum. By the availability of this detailed atomic description, there exists the opportunity to tailor chemical and mechanical processes involving metakaolin at the atomic level to obtain optimal performance at the macro-scale.

  15. Solving Rectangular Fuzzy Games through

    Directory of Open Access Journals (Sweden)

    Chaudhuri Arindam

    2017-12-01

    Full Text Available Fuzzy set theory has been applied in many fields such as operations research, control theory and decision sciences. In particular, an application of this theory in decision making problems has a remarkable significance. In this paper, we consider a solution of rectangular fuzzy game with pay-off as imprecise numbers instead of crisp numbers viz., interval and LR-type trapezoidal fuzzy numbers. The solution of such fuzzy games with pure strategies by minimax-maximin principle is discussed. The algebraic method to solve 2 × 2 fuzzy games without saddle point by using mixed strategies is also illustrated. Here m × n payoff matrix is reduced to 2 × 2 pay-off matrix by dominance method. This fact is illustrated by means of numerical example.

  16. Adaptive Problem Solving by Analogy

    Science.gov (United States)

    2013-07-01

    gestalt   psychologist  developed  a  number of  rules which prescribe when  and how  visual   features  are be bound  together.  There is however a...tracking,  visualization   of the working memory, and a script language for setting‐up and controlling simulations.  Summary and dissemination are presented in...set of  visual   features belong  to  the  same object).  The binding problem  is  also  relevant  to  problem solving as long as prior to finding a

  17. Community-powered problem solving.

    Science.gov (United States)

    Gouillart, Francis; Billings, Douglas

    2013-04-01

    Traditionally, companies have managed their constituencies with specific processes: marketing to customers, procuring from vendors, developing HR policies for employees, and so on. The problem is, such processes focus on repeatability and compliance, so they can lead to stagnation. Inviting your constituencies to collectively help you solve problems and exploit opportunities--"co-creation"--is a better approach. It allows you to continually tap the skills and insights of huge numbers of stakeholders and develop new ways to produce value for all. The idea is to provide stakeholders with platforms (physical and digital forums) on which they can interact, get them to start exploring new experiences and connections, and let the system grow organically. A co-creation initiative by a unit of Becton, Dickinson and Company demonstrates how this works. A global leader in syringes, BD set out to deepen its ties with hospital customers and help them reduce the incidence of infections from unsafe injection and syringe disposal practices. The effort began with a cross-functional internal team, brought in the hospital procurement and supply managers BD had relationships with, and then reached out to hospitals' infection-prevention and occupational health leaders. Eventually product designers, nurses, sustainability staffers, and even hospital CFOs were using the platform, contributing data that generated new best practices and reduced infections.

  18. An Insight into the Interaction Mode Between CheB and Chemoreceptor from Two Crystal Structures of CheB Methylesterase Catalytic Domain

    Energy Technology Data Exchange (ETDEWEB)

    K Cho; B Crane; S Park

    2011-12-31

    We have determined 2.2 {angstrom} resolution crystal structure of Thermotoga maritima CheB methylesterase domain to provide insight into the interaction mode between CheB and chemoreceptors. T. maritima CheB methylesterase domain has identical topology of a modified doubly-wound {alpha}/{beta} fold that was observed from the previously reported Salmonella typhimurium counterpart, but the analysis of the electrostatic potential surface near the catalytic triad indicated considerable charge distribution difference. As the CheB demethylation consensus sites of the chemoreceptors, the CheB substrate, are not uniquely conserved between T. maritima and S. typhimurium, such surfaces with differing electrostatic properties may reflect CheB regions that mediate protein-protein interaction. Via the computational docking of the two T. maritima and S. typhimurium CheB structures to the respective T. maritima and Escherichia coli chemoreceptors, we propose a CheB:chemoreceptor interaction mode.

  19. Students’ difficulties in probabilistic problem-solving

    Science.gov (United States)

    Arum, D. P.; Kusmayadi, T. A.; Pramudya, I.

    2018-03-01

    There are many errors can be identified when students solving mathematics problems, particularly in solving the probabilistic problem. This present study aims to investigate students’ difficulties in solving the probabilistic problem. It focuses on analyzing and describing students errors during solving the problem. This research used the qualitative method with case study strategy. The subjects in this research involve ten students of 9th grade that were selected by purposive sampling. Data in this research involve students’ probabilistic problem-solving result and recorded interview regarding students’ difficulties in solving the problem. Those data were analyzed descriptively using Miles and Huberman steps. The results show that students have difficulties in solving the probabilistic problem and can be divided into three categories. First difficulties relate to students’ difficulties in understanding the probabilistic problem. Second, students’ difficulties in choosing and using appropriate strategies for solving the problem. Third, students’ difficulties with the computational process in solving the problem. Based on the result seems that students still have difficulties in solving the probabilistic problem. It means that students have not able to use their knowledge and ability for responding probabilistic problem yet. Therefore, it is important for mathematics teachers to plan probabilistic learning which could optimize students probabilistic thinking ability.

  20. Conceptual problem solving in high school physics

    OpenAIRE

    Jennifer L. Docktor; Natalie E. Strand; José P. Mestre; Brian H. Ross

    2015-01-01

    Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an instructional approach called Conceptual Problem Solving (CPS) which guides students to identify principles, justify their use, and plan their solution in w...

  1. An Integrated Architecture for Engineering Problem Solving

    National Research Council Canada - National Science Library

    Pisan, Yusuf

    1998-01-01

    .... This thesis describes the Integrated Problem Solving Architecture (IPSA) that combines qualitative, quantitative and diagrammatic reasoning skills to produce annotated solutions to engineering problems...

  2. Mobile serious games for collaborative problem solving.

    Science.gov (United States)

    Sanchez, Jaime; Mendoza, Claudia; Salinas, Alvaro

    2009-01-01

    This paper presents the results obtained from the implementation of a series of learning activities based on mobile serious games (MSG) for the development of problem-solving and collaborative skills in Chilean 8th grade students. Three MSGs were developed and played by teams of four students, who had to solve the problems posed by the game collaboratively. The data shows that the experimental group had a higher perception of their own skills of collaboration and of the plan execution dimension of problem solving than the control group, providing empirical evidence regarding the contribution of MSGs to the development of collaborative problem-solving skills.

  3. Distance Measurement Solves Astrophysical Mysteries

    Science.gov (United States)

    2003-08-01

    Location, location, and location. The old real-estate adage about what's really important proved applicable to astrophysics as astronomers used the sharp radio "vision" of the National Science Foundation's Very Long Baseline Array (VLBA) to pinpoint the distance to a pulsar. Their accurate distance measurement then resolved a dispute over the pulsar's birthplace, allowed the astronomers to determine the size of its neutron star and possibly solve a mystery about cosmic rays. "Getting an accurate distance to this pulsar gave us a real bonanza," said Walter Brisken, of the National Radio Astronomy Observatory (NRAO) in Socorro, NM. Monogem Ring The Monogem Ring, in X-Ray Image by ROSAT satellite CREDIT: Max-Planck Institute, American Astronomical Society (Click on Image for Larger Version) The pulsar, called PSR B0656+14, is in the constellation Gemini, and appears to be near the center of a circular supernova remnant that straddles Gemini and its neighboring constellation, Monoceros, and is thus called the Monogem Ring. Since pulsars are superdense, spinning neutron stars left over when a massive star explodes as a supernova, it was logical to assume that the Monogem Ring, the shell of debris from a supernova explosion, was the remnant of the blast that created the pulsar. However, astronomers using indirect methods of determining the distance to the pulsar had concluded that it was nearly 2500 light-years from Earth. On the other hand, the supernova remnant was determined to be only about 1000 light-years from Earth. It seemed unlikely that the two were related, but instead appeared nearby in the sky purely by a chance juxtaposition. Brisken and his colleagues used the VLBA to make precise measurements of the sky position of PSR B0656+14 from 2000 to 2002. They were able to detect the slight offset in the object's apparent position when viewed from opposite sides of Earth's orbit around the Sun. This effect, called parallax, provides a direct measurement of

  4. Solving Cubic Equations by Polynomial Decomposition

    Science.gov (United States)

    Kulkarni, Raghavendra G.

    2011-01-01

    Several mathematicians struggled to solve cubic equations, and in 1515 Scipione del Ferro reportedly solved the cubic while participating in a local mathematical contest, but did not bother to publish his method. Then it was Cardano (1539) who first published the solution to the general cubic equation in his book "The Great Art, or, The Rules of…

  5. Problem Solving Methods in Engineering Design

    DEFF Research Database (Denmark)

    Hartvig, Susanne C

    1999-01-01

    This short paper discusses typical engineering tasks and problem solving methods, based on a field study of engineering tasks at a Danish engineering firm. The field study has identified ten classes of design tasks and in this paper these classes are related to problem solving methods...

  6. Creativity and Insight in Problem Solving

    Science.gov (United States)

    Golnabi, Laura

    2016-01-01

    This paper analyzes the thought process involved in problem solving and its categorization as creative thinking as defined by psychologist R. Weisberg (2006). Additionally, the notion of insight, sometimes present in unconscious creative thinking and often leading to creative ideas, is discussed in the context of geometry problem solving. In…

  7. Teaching Problem Solving: An Instructional Design Strategy.

    Science.gov (United States)

    Ross, John A.; Maynes, Florence J.

    1983-01-01

    Instructional design strategy for improving problem solving is presented. The strategy entails selecting an appropriate domain of problem-solving tasks, learning hierarchies, teaching methods and assembling of learning materials, and designing teacher training and evaluation. Obstacles to be overcome and directions for future research are…

  8. Teaching Problem-Solving. Informal Series/43.

    Science.gov (United States)

    Ross, John A.; Maynes, Florence J.

    This monograph is designed to provide practical classroom suggestions, including sample lesson plans, to show how teachers can improve the problem-solving competence of students at all educational and ability levels. The examples provided show that problem-solving instruction can be integrated with teaching the content of particular topics. While…

  9. Mathematical problem solving in primary school

    NARCIS (Netherlands)

    Kolovou, A.

    2011-01-01

    A student is engaged in (non-routine) problem solving when there is no clear pathway to the solution. In contrast to routine problems, non-routine ones cannot be solved through the direct application of a standard procedure. Consider the following problem: In a quiz you get two points for each

  10. Conceptual Problem Solving in High School Physics

    Science.gov (United States)

    Docktor, Jennifer L.; Strand, Natalie E.; Mestre, José P.; Ross, Brian H.

    2015-01-01

    Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an…

  11. Solving applied mathematical problems with Matlab

    CERN Document Server

    Xue, Dingyu

    2008-01-01

    Computer Mathematics Language-An Overview. Fundamentals of MATLAB Programming. Calculus Problems. MATLAB Computations of Linear Algebra Problems. Integral Transforms and Complex Variable Functions. Solutions to Nonlinear Equations and Optimization Problems. MATLAB Solutions to Differential Equation Problems. Solving Interpolations and Approximations Problems. Solving Probability and Mathematical Statistics Problems. Nontraditional Solution Methods for Mathematical Problems.

  12. Measuring Problem Solving Skills in "Portal 2"

    Science.gov (United States)

    Shute, Valerie J.; Wang, Lubin

    2013-01-01

    This paper examines possible improvement to problem solving skills as a function of playing the video game "Portal 2." Stealth assessment is used in the game to evaluate students' problem solving abilities--specifically basic and flexible rule application. The stealth assessment measures will be validated against commonly accepted…

  13. Concept mapping instrumental support for problem solving

    NARCIS (Netherlands)

    Stoyanov, S.; Stoyanov, Slavi; Kommers, Petrus A.M.

    2008-01-01

    The main theoretical position of this paper is that it is the explicit problem-solving support in concept mapping software that produces a stronger effect in problem-solving performance than the implicit support afforded by the graphical functionality of concept mapping software. Explicit

  14. Synthesizing Huber's Problem Solving and Kolb's Learning Cycle: A Balanced Approach to Technical Problem Solving

    Science.gov (United States)

    Kamis, Arnold; Khan, Beverly K.

    2009-01-01

    How do we model and improve technical problem solving, such as network subnetting? This paper reports an experimental study that tested several hypotheses derived from Kolb's experiential learning cycle and Huber's problem solving model. As subjects solved a network subnetting problem, they mapped their mental processes according to Huber's…

  15. Pre-Service Class Teacher' Ability in Solving Mathematical Problems and Skills in Solving Daily Problems

    Science.gov (United States)

    Aljaberi, Nahil M.; Gheith, Eman

    2016-01-01

    This study aims to investigate the ability of pre-service class teacher at University of Petrain solving mathematical problems using Polya's Techniques, their level of problem solving skills in daily-life issues. The study also investigates the correlation between their ability to solve mathematical problems and their level of problem solving…

  16. Lesion mapping of social problem solving

    Science.gov (United States)

    Colom, Roberto; Paul, Erick J.; Chau, Aileen; Solomon, Jeffrey; Grafman, Jordan H.

    2014-01-01

    Accumulating neuroscience evidence indicates that human intelligence is supported by a distributed network of frontal and parietal regions that enable complex, goal-directed behaviour. However, the contributions of this network to social aspects of intellectual function remain to be well characterized. Here, we report a human lesion study (n = 144) that investigates the neural bases of social problem solving (measured by the Everyday Problem Solving Inventory) and examine the degree to which individual differences in performance are predicted by a broad spectrum of psychological variables, including psychometric intelligence (measured by the Wechsler Adult Intelligence Scale), emotional intelligence (measured by the Mayer, Salovey, Caruso Emotional Intelligence Test), and personality traits (measured by the Neuroticism-Extraversion-Openness Personality Inventory). Scores for each variable were obtained, followed by voxel-based lesion–symptom mapping. Stepwise regression analyses revealed that working memory, processing speed, and emotional intelligence predict individual differences in everyday problem solving. A targeted analysis of specific everyday problem solving domains (involving friends, home management, consumerism, work, information management, and family) revealed psychological variables that selectively contribute to each. Lesion mapping results indicated that social problem solving, psychometric intelligence, and emotional intelligence are supported by a shared network of frontal, temporal, and parietal regions, including white matter association tracts that bind these areas into a coordinated system. The results support an integrative framework for understanding social intelligence and make specific recommendations for the application of the Everyday Problem Solving Inventory to the study of social problem solving in health and disease. PMID:25070511

  17. Problem-solving competency of nursing graduates.

    Science.gov (United States)

    Uys, Leana R; Van Rhyn, Lily L; Gwele, Nomthandaso S; McInerney, Patricia; Tanga, Tobeka

    2004-12-01

    This paper reports a study describing and evaluating the outcomes of problem-based learning (PBL) programmes in nursing schools in South Africa in terms of the competence of graduates to solve problem in actual clinical settings, and comparing this competence with that of graduates from non-PBL programmes. The nursing literature tends to equate problem-solving with patient-centred problems or the nursing process. However, it is also a skill used in managing the work role, working in a team and managing a health care unit. Problem-solving refers to the process of selectively attending to information in a patient care setting. The investigation of problem-solving in nursing is complicated by the complex relationships between different cognitive processes. A qualitative evaluation study, descriptive and comparative in nature, was carried out. In-depth interviews were held with graduates and their supervisors, asking them to identify problem-solving incidents in which they had been involved. Template analysis style and Benner's interpretive approach were used to analyse the data. The majority of the incidents described by the graduates (84%) were graded at the advanced beginner level or above. The majority of incidents at the novice level came from the non-PBL group. 'Using people skills' and 'being assertive' were the two problem-solving strategies most often used. The PBL group fared better than the non-problem-based group in the level of their problem-solving ability. The findings of this study suggest that further research is warranted into the problem-solving abilities of PBL graduates, their personal development over time and at different stages of practice. In addition, it would be interesting to follow the development of their problem-solving abilities over time.

  18. The ideal science student and problem solving

    Science.gov (United States)

    Sullivan, Florence R.

    2005-09-01

    The purpose of this dissertation was to examine the relationship between students' social mental models of the ideal science student, science epistemological beliefs, problem solving strategies used, and problem solving ability in a robotics environment. Participants were twenty-six academically advanced eleven and twelve year old students attending the Center for Talented Youth summer camp. Survey data was collected from the students including demographic background, views of the ideal science student, and science epistemological beliefs. Students also solved a robotics challenge. This problem solving session was videotaped and students were asked to think aloud as they solved the problem. Two social mental models were identified, a traits-based social mental model and a robust social mental model. A significant association was found between social mental model group and strategy usage. The robust social mental model group is more likely to use domain specific strategies than the traits-based group. Additionally, the robust social mental model group achieved significantly higher scores on their final solution than the traits-based social mental model group. Science epistemological beliefs do not appear to be associated with students' social mental model of the ideal science student. While students with a puzzle-solver view of science were more likely to use domain specific strategies in the planning phase of the problem solving session, there was no significant difference in problem solving ability between this group and students who have a dynamic view of the nature of science knowledge. This difference in strategy usage and problem solving performance may be due to a difference in the students' views of learning and cognition. The robust social mental model group evidenced a situative view of learning and cognition. These students made excellent use of the tools available in the task environment. The traits-based social mental model group displayed an

  19. Assertiveness and problem solving in midwives.

    Science.gov (United States)

    Yurtsal, Zeliha Burcu; Özdemir, Levent

    2015-01-01

    Midwifery profession is required to bring solutions to problems and a midwife is expected to be an assertive person and to develop midwifery care. This study was planned to examine the relationship between assertiveness and problem-solving skills of midwives. This cross-sectional study was conducted with 201 midwives between July 2008 and February 2009 in the city center of Sivas. The Rathus Assertiveness Schedule (RAS) and Problem Solving Inventory (PSI) were used to determine the level of assertiveness and problem-solving skills of midwives. Statistical methods were used as mean, standard deviation, percentage, Student's T, ANOVA and Tukey HSD, Kruskal Wallis, Fisher Exact, Pearson Correlation and Chi-square tests and P < 0.05. The RAS mean scores and the PSI mean scores showed statistically significant differences in terms of a midwife's considering herself as a member of the health team, expressing herself within the health care team, being able to say "no" when necessary, cooperating with her colleagues, taking part in problem-solving skills training. A statistically significant negative correlation was found between the RAS and PSI scores. The RAS scores decreased while the problem-solving scores increased (r: -0451, P < 0.01). There were significant statistical differences between assertiveness levels and problem solving skills of midwives, and midwives who were assertive solved their problems better than did others. Assertiveness and problem-solving skills training will contribute to the success of the midwifery profession. Midwives able to solve problems, and display assertive behaviors will contribute to the development of midwifery profession.

  20. Improving mathematical problem solving : A computerized approach

    NARCIS (Netherlands)

    Harskamp, EG; Suhre, CJM

    Mathematics teachers often experience difficulties in teaching students to become skilled problem solvers. This paper evaluates the effectiveness of two interactive computer programs for high school mathematics problem solving. Both programs present students with problems accompanied by instruction

  1. Exact Algorithms for Solving Stochastic Games

    DEFF Research Database (Denmark)

    Hansen, Kristoffer Arnsfelt; Koucky, Michal; Lauritzen, Niels

    2012-01-01

    Shapley's discounted stochastic games, Everett's recursive games and Gillette's undiscounted stochastic games are classical models of game theory describing two-player zero-sum games of potentially infinite duration. We describe algorithms for exactly solving these games....

  2. How to solve applied mathematics problems

    CERN Document Server

    Moiseiwitsch, B L

    2011-01-01

    This workbook bridges the gap between lectures and practical applications, offering students of mathematics, engineering, and physics the chance to practice solving problems from a wide variety of fields. 2011 edition.

  3. Deterministic equation solving over finite fields

    NARCIS (Netherlands)

    Woestijne, Christiaan Evert van de

    2006-01-01

    It is shown how to solve diagonal forms in many variables over finite fields by means of a deterministic efficient algorithm. Applications to norm equations, quadratic forms, and elliptic curves are given.

  4. Physics: Quantum problems solved through games

    Science.gov (United States)

    Maniscalco, Sabrina

    2016-04-01

    Humans are better than computers at performing certain tasks because of their intuition and superior visual processing. Video games are now being used to channel these abilities to solve problems in quantum physics. See Letter p.210

  5. The art and science of problem solving

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    2005-01-01

    In this paper we will document that real-life problem solving in complex situations demands both rational (scientific) and intuitive (artistic) thinking. First, the concepts of art and science will be discussed; differences and similarities will be enhanced. Thereafter the concept of group problem...... solving facilitation both as science and art will be presented. A case study related to examination's planning will be discussed to illustrate the main concepts in practice. In addition, other cases studies will also be shortly presented....

  6. Methods for solving mathematical physics problems

    CERN Document Server

    Agoshkov, VI; Shutyaev, VP

    2006-01-01

    The book examines the classic and generally accepted methods for solving mathematical physics problems (method of the potential theory, the eigenfunction method, integral transformation methods, discretisation characterisation methods, splitting methods). A separate chapter is devoted to methods for solving nonlinear equations. The book offers a large number of examples of how these methods are applied to the solution of specific mathematical physics problems, applied in the areas of science and social activities, such as energy, environmental protection, hydrodynamics, theory of elasticity, etc.

  7. Conceptual problem solving in high school physics

    Directory of Open Access Journals (Sweden)

    Jennifer L. Docktor

    2015-09-01

    Full Text Available Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an instructional approach called Conceptual Problem Solving (CPS which guides students to identify principles, justify their use, and plan their solution in writing before solving a problem. The CPS approach was implemented by high school physics teachers at three schools for major theorems and conservation laws in mechanics and CPS-taught classes were compared to control classes taught using traditional problem solving methods. Information about the teachers’ implementation of the approach was gathered from classroom observations and interviews, and the effectiveness of the approach was evaluated from a series of written assessments. Results indicated that teachers found CPS easy to integrate into their curricula, students engaged in classroom discussions and produced problem solutions of a higher quality than before, and students scored higher on conceptual and problem solving measures.

  8. Conceptual problem solving in high school physics

    Science.gov (United States)

    Docktor, Jennifer L.; Strand, Natalie E.; Mestre, José P.; Ross, Brian H.

    2015-12-01

    Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an instructional approach called Conceptual Problem Solving (CPS) which guides students to identify principles, justify their use, and plan their solution in writing before solving a problem. The CPS approach was implemented by high school physics teachers at three schools for major theorems and conservation laws in mechanics and CPS-taught classes were compared to control classes taught using traditional problem solving methods. Information about the teachers' implementation of the approach was gathered from classroom observations and interviews, and the effectiveness of the approach was evaluated from a series of written assessments. Results indicated that teachers found CPS easy to integrate into their curricula, students engaged in classroom discussions and produced problem solutions of a higher quality than before, and students scored higher on conceptual and problem solving measures.

  9. The effect of learning environments based on problem solving on students’ achievements of problem solving

    Directory of Open Access Journals (Sweden)

    Ilhan Karatas

    2013-07-01

    Full Text Available Problem solving is recognized as an important life skill involving a range of processes including analyzing, interpreting, reasoning, predicting, evaluating and reflecting. For that reason educating students as efficient problem solvers is an important role of mathematics education. Problem solving skill is the centre of mathematics curriculum. Students’ gaining of that skill in school mathematics is closely related with the learning environment to be formed and the roles given to the students. The aim of this study is to create a problem solving based learning environment to enhance the students’ problem solving skill. Within this scope, students’ practiced activities and problems that provide them to proceed in Polya (1945’s problem solving phases and throughout the study, students’ success in problem solving have been evaluated. While experimental group students received problem solving based learning environment performed, control group students have continued their present program in this quise-experimental study. Eleven problem solving activities were given to the students at the beginning, middle and end of the study and the students’ performances were analyzed based on problem solving phases. The findings illustrated that the experimental group students’ success in problem solving activities has increased while the control group students’ success has not changed significantly.

  10. The Effect of Learning Environments Based on Problem Solving on Students’ Achievements of Problem Solving

    Directory of Open Access Journals (Sweden)

    Ilhan KARATAS

    2013-07-01

    Full Text Available Problem solving is recognized as an important life skill involving a range of processes including analyzing, interpreting, reasoning, predicting, evaluating and reflecting. For that reason educatingstudents as efficient problem solvers is an important role of mathematics education. Problem solving skill is the centre of mathematics curriculum. Students’ gaining of that skill in school mathematics is closely related with the learning environment to beformed and the roles given to the students. The aim of this study is to create a problem solving based learning environment to enhance the students’ problem solving skill. Within this scope, students’practiced activities and problems that provide them to proceed in Polya (1945’s problem solving phases and throughout the study, students’ success in problem solving have been evaluated. While experimental group students received problem solving based learning environment performed, control group students have continued their present program in this quise1experimental study. Eleven problem solving activities were given to the students at the beginning, middle and end of the study and the students’ performances wereanalyzed based on problem solving phases. The findings illustrated that the experimental group students’ success in problem solving activities has increased while the control group students’ success has not changed significantly.

  11. Development of a problem solving evaluation instrument; untangling of specific problem solving assets

    Science.gov (United States)

    Adams, Wendy Kristine

    The purpose of my research was to produce a problem solving evaluation tool for physics. To do this it was necessary to gain a thorough understanding of how students solve problems. Although physics educators highly value problem solving and have put extensive effort into understanding successful problem solving, there is currently no efficient way to evaluate problem solving skill. Attempts have been made in the past; however, knowledge of the principles required to solve the subject problem are so absolutely critical that they completely overshadow any other skills students may use when solving a problem. The work presented here is unique because the evaluation tool removes the requirement that the student already have a grasp of physics concepts. It is also unique because I picked a wide range of people and picked a wide range of tasks for evaluation. This is an important design feature that helps make things emerge more clearly. This dissertation includes an extensive literature review of problem solving in physics, math, education and cognitive science as well as descriptions of studies involving student use of interactive computer simulations, the design and validation of a beliefs about physics survey and finally the design of the problem solving evaluation tool. I have successfully developed and validated a problem solving evaluation tool that identifies 44 separate assets (skills) necessary for solving problems. Rigorous validation studies, including work with an independent interviewer, show these assets identified by this content-free evaluation tool are the same assets that students use to solve problems in mechanics and quantum mechanics. Understanding this set of component assets will help teachers and researchers address problem solving within the classroom.

  12. Solving work-related ethical problems.

    Science.gov (United States)

    Laukkanen, Laura; Suhonen, Riitta; Leino-Kilpi, Helena

    2016-12-01

    Nurse managers are responsible for solving work-related ethical problems to promote a positive ethical culture in healthcare organizations. The aim of this study was to describe the activities that nurse managers use to solve work-related ethical problems. The ultimate aim was to enhance the ethical awareness of all nurse managers. The data for this descriptive cross-sectional survey were analyzed through inductive content analysis and quantification. Participants and research context: The data were collected in 2011 using a questionnaire that included an open-ended question and background factors. Participants were nurse managers working in Finnish healthcare organizations (n = 122). Ethical considerations: Permission for the study was given by the Finnish Association of Academic Managers and Experts of Health Sciences. Nurse managers identified a variety of activities they use to solve work-related ethical problems: discussion (30%), cooperation (25%), work organization (17%), intervention (10%), personal values (9%), operational models (4%), statistics and feedback (4%), and personal examples (1%). However, these activities did not follow any common or systematic model. In the future, nurse managers need a more systematic approach to solve ethical problems. It is important to establish new kinds of ethics structures in organizations, such as a common, systematic ethical decision-making model and an ethics club for nurse manager problems, to support nurse managers in solving work-related ethical problems.

  13. Error analysis in solving mathematical problems

    Directory of Open Access Journals (Sweden)

    Geovana Luiza Kliemann

    2017-12-01

    Full Text Available This paper presents a survey carried out within the Centre for Education Programme, in order to assist in improving the quality of the teaching and learning of Mathematics in Primary Education. From the study of the evaluative systems that constitute the scope of the research project, it was found that their focus is solving problems, and from this point, it began the development of several actions with the purpose of assisting the students in the process of solving them. One of these actions objected to analyze the errors presented by students in the 5th year in the interpretation, understanding, and problem-solving. We describe three games developed in six schools, with questions drawn from the “Prova Brasil” performed in previous years, in objective to diagnose the main difficulties presented by the students in solving the problems, besides helping them to verify possibilities to overcome such gaps. To reach the proposed objectives, a qualitative study was carried out in which the researchers were constantly involved during the process. After each meeting, there was an analysis of the responses developed to classify the errors in different categories. It was found that most students attended succeeded in solving the proposed problems, and major errors presented are related to the difficulty of interpretation.

  14. On Teaching Problem Solving in School Mathematics

    Directory of Open Access Journals (Sweden)

    Erkki Pehkonen

    2013-12-01

    Full Text Available The article begins with a brief overview of the situation throughout the world regarding problem solving. The activities of the ProMath group are then described, as the purpose of this international research group is to improve mathematics teaching in school. One mathematics teaching method that seems to be functioning in school is the use of open problems (i.e., problem fields. Next we discuss the objectives of the Finnish curriculum that are connected with problem solving. Some examples and research results are taken from a Finnish–Chilean research project that monitors the development of problem-solving skills in third grade pupils. Finally, some ideas on “teacher change” are put forward. It is not possible to change teachers, but only to provide hints for possible change routes: the teachers themselves should work out the ideas and their implementation.

  15. Naps improve new walkers' locomotor problem solving.

    Science.gov (United States)

    Berger, Sarah E; Scher, Anat

    2017-10-01

    In this first study of the impact of sleep on infants' problem solving of a locomotor task, 28 newly walking infants who were within a week of having given up crawling trained to navigate a shoulder-height tunnel to reach a caregiver waiting at the end. During the transitional window between crawling and walking, infants are reluctant to return to crawling, making this task uniquely challenging. Infants were randomly assigned to either nap or stay awake during a delay between training and a later test session. For the Nap group, efficiency of problem solving improved from training to test, but there was no change for the No Nap group. These findings suggest that for newly walking infants, sleep facilitates learning to solve a novel motor problem. Published by Elsevier Inc.

  16. Methods of solving sequence and series problems

    CERN Document Server

    Grigorieva, Ellina

    2016-01-01

    This book aims to dispel the mystery and fear experienced by students surrounding sequences, series, convergence, and their applications. The author, an accomplished female mathematician, achieves this by taking a problem solving approach, starting with fascinating problems and solving them step by step with clear explanations and illuminating diagrams. The reader will find the problems interesting, unusual, and fun, yet solved with the rigor expected in a competition. Some problems are taken directly from mathematics competitions, with the name and year of the exam provided for reference. Proof techniques are emphasized, with a variety of methods presented. The text aims to expand the mind of the reader by often presenting multiple ways to attack the same problem, as well as drawing connections with different fields of mathematics. Intuitive and visual arguments are presented alongside technical proofs to provide a well-rounded methodology. With nearly 300 problems including hints, answers, and solutions,Met...

  17. Vacuum engineering, calculations, formulas, and solved exercises

    CERN Document Server

    Berman, Armand

    1992-01-01

    This book was written with two main objectives in mind-to summarize and organize the vast material of vacuum technology in sets of useful formulas, and to provide a collection of worked out exercises showing how to use these formulas for solving technological problems. It is an ideal reference source for those with little time to devote to a full mathematical treatment of the many problems issued in vacuum practice, but who have a working knowledge of the essentials of vacuum technology, elementary physics, and mathematics. This time saving book employs a problem-solving approach throughout, p

  18. Student Obstacles in Solving Algebraic Thinking Problems

    Science.gov (United States)

    Andini, W.; Suryadi, D.

    2017-09-01

    The aim of this research is to analize the student obstacles on solving algebraic thinking problems in low grades elementary school. This research is a preliminary qualitative research, and involved 66 students of grade 3 elementary school. From the analysis student test results, most of student experience difficulty in solving algebraic thinking problems. The main obstacle is the student’s difficulty in understanding the problem of generalizing the pattern because the students are not accustomed to see the rules that exist in generalize the pattern.

  19. A Novel Approach for Solving Semidefinite Programs

    Directory of Open Access Journals (Sweden)

    Hong-Wei Jiao

    2014-01-01

    Full Text Available A novel linearizing alternating direction augmented Lagrangian approach is proposed for effectively solving semidefinite programs (SDP. For every iteration, by fixing the other variables, the proposed approach alternatively optimizes the dual variables and the dual slack variables; then the primal variables, that is, Lagrange multipliers, are updated. In addition, the proposed approach renews all the variables in closed forms without solving any system of linear equations. Global convergence of the proposed approach is proved under mild conditions, and two numerical problems are given to demonstrate the effectiveness of the presented approach.

  20. Algorithms to solve the Sutherland model

    OpenAIRE

    Langmann, Edwin

    2001-01-01

    We give a self-contained presentation and comparison of two different algorithms to explicitly solve quantum many body models of indistinguishable particles moving on a circle and interacting with two-body potentials of $1/\\sin^2$-type. The first algorithm is due to Sutherland and well-known; the second one is a limiting case of a novel algorithm to solve the elliptic generalization of the Sutherland model. These two algorithms are different in several details. We show that they are equivalen...

  1. Dreams and creative problem-solving.

    Science.gov (United States)

    Barrett, Deirdre

    2017-10-01

    Dreams have produced art, music, novels, films, mathematical proofs, designs for architecture, telescopes, and computers. Dreaming is essentially our brain thinking in another neurophysiologic state-and therefore it is likely to solve some problems on which our waking minds have become stuck. This neurophysiologic state is characterized by high activity in brain areas associated with imagery, so problems requiring vivid visualization are also more likely to get help from dreaming. This article reviews great historical dreams and modern laboratory research to suggest how dreams can aid creativity and problem-solving. © 2017 New York Academy of Sciences.

  2. The Effect of Learning Environments Based on Problem Solving on Students' Achievements of Problem Solving

    Science.gov (United States)

    Karatas, Ilhan; Baki, Adnan

    2013-01-01

    Problem solving is recognized as an important life skill involving a range of processes including analyzing, interpreting, reasoning, predicting, evaluating and reflecting. For that reason educating students as efficient problem solvers is an important role of mathematics education. Problem solving skill is the centre of mathematics curriculum.…

  3. Students' Errors in Solving the Permutation and Combination Problems Based on Problem Solving Steps of Polya

    Science.gov (United States)

    Sukoriyanto; Nusantara, Toto; Subanji; Chandra, Tjang Daniel

    2016-01-01

    This article was written based on the results of a study evaluating students' errors in problem solving of permutation and combination in terms of problem solving steps according to Polya. Twenty-five students were asked to do four problems related to permutation and combination. The research results showed that the students still did a mistake in…

  4. Cooperative learning, problem solving and mediating artifacts ...

    African Journals Online (AJOL)

    The present study deals with the influence of cooperative learning on the ability of students to solve the problems. The study also concerns the introduction of mathematical mediating artifacts as factors which effect the learning of mathematics by students. Experimental research method of pre-test and post-test types was ...

  5. Solving Word Problems: As Easy As PIES!

    Science.gov (United States)

    Heater, Mary Jane; Howard, Lori A.; Linz, Ed

    2012-01-01

    Many students are challenged when tasked to complete a word problem. While they may know the procedural steps to solve an equation, translating a word problem into an appropriate equation and producing a solution may often cause students to become confused or unwilling to try. This article provides a potential solution for teachers by discussing…

  6. Mental Imagery in Creative Problem Solving.

    Science.gov (United States)

    Polland, Mark J.

    In order to investigate the relationship between mental imagery and creative problem solving, a study of 44 separate accounts reporting mental imagery experiences associated with creative discoveries were examined. The data included 29 different scientists, among them Albert Einstein and Stephen Hawking, and 9 artists, musicians, and writers,…

  7. Solving Problems with the Percentage Bar

    Science.gov (United States)

    van Galen, Frans; van Eerde, Dolly

    2013-01-01

    At the end of primary school all children more of less know what a percentage is, but yet they often struggle with percentage problems. This article describes a study in which students of 13 and 14 years old were given a written test with percentage problems and a week later were interviewed about the way they solved some of these problems. In a…

  8. Three-M in Word Problem Solving

    Science.gov (United States)

    Hajra, Sayonita Ghosh; Kofman, Victoria

    2018-01-01

    We describe three activities that help undergraduates (pre-service teachers) to develop scientific vocabulary on measurable attributes and units of measurement. Measurable attributes are important features in understanding a word problem and solving the problem. These activities help students comprehend word problems better by identifying…

  9. A Versatile Technique for Solving Quintic Equations

    Science.gov (United States)

    Kulkarni, Raghavendra G.

    2006-01-01

    In this paper we present a versatile technique to solve several types of solvable quintic equations. In the technique described here, the given quintic is first converted to a sextic equation by adding a root, and the resulting sextic equation is decomposed into two cubic polynomials as factors in a novel fashion. The resultant cubic equations are…

  10. Problem solving environment for distributed interactive applications

    NARCIS (Netherlands)

    Rycerz, K.; Bubak, M.; Sloot, P.; Getov, V.; Gorlatch, S.; Bubak, M.; Priol, T.

    2008-01-01

    Interactive Problem Solving Environments (PSEs) offer an integrated approach for constructing and running complex systems, such as distributed simulation systems. To achieve efficient execution of High Level Architecture (HLA)-based distributed interactive simulations on the Grid, we introduce a PSE

  11. The Use of Transformations in Solving Equations

    Science.gov (United States)

    Libeskind, Shlomo

    2010-01-01

    Many workshops and meetings with the US high school mathematics teachers revealed a lack of familiarity with the use of transformations in solving equations and problems related to the roots of polynomials. This note describes two transformational approaches to the derivation of the quadratic formula as well as transformational approaches to…

  12. Neural Network to Solve Concave Games

    OpenAIRE

    Liu, Zixin; Wang, Nengfa

    2014-01-01

    The issue on neural network method to solve concave games is concerned. Combined with variational inequality, Ky Fan inequality, and projection equation, concave games are transformed into a neural network model. On the basis of the Lyapunov stable theory, some stability results are also given. Finally, two classic games’ simulation results are given to illustrate the theoretical results.

  13. Managing Element Interactivity in Equation Solving

    Science.gov (United States)

    Ngu, Bing Hiong; Phan, Huy P.; Yeung, Alexander Seeshing; Chung, Siu Fung

    2018-01-01

    Between two popular teaching methods (i.e., balance method vs. inverse method) for equation solving, the main difference occurs at the operational line (e.g., +2 on both sides vs. -2 becomes +2), whereby it alters the state of the equation and yet maintains its equality. Element interactivity occurs on both sides of the equation in the balance…

  14. Insightful problem solving in an Asian elephant.

    Directory of Open Access Journals (Sweden)

    Preston Foerder

    Full Text Available The "aha" moment or the sudden arrival of the solution to a problem is a common human experience. Spontaneous problem solving without evident trial and error behavior in humans and other animals has been referred to as insight. Surprisingly, elephants, thought to be highly intelligent, have failed to exhibit insightful problem solving in previous cognitive studies. We tested whether three Asian elephants (Elephas maximus would use sticks or other objects to obtain food items placed out-of-reach and overhead. Without prior trial and error behavior, a 7-year-old male Asian elephant showed spontaneous problem solving by moving a large plastic cube, on which he then stood, to acquire the food. In further testing he showed behavioral flexibility, using this technique to reach other items and retrieving the cube from various locations to use as a tool to acquire food. In the cube's absence, he generalized this tool utilization technique to other objects and, when given smaller objects, stacked them in an attempt to reach the food. The elephant's overall behavior was consistent with the definition of insightful problem solving. Previous failures to demonstrate this ability in elephants may have resulted not from a lack of cognitive ability but from the presentation of tasks requiring trunk-held sticks as potential tools, thereby interfering with the trunk's use as a sensory organ to locate the targeted food.

  15. Developing Creative Problem Solving in Civil Engineering.

    Science.gov (United States)

    Barker, Dennis

    1986-01-01

    A British polytechnic's civil engineering course emphasizing creative problem solving, begun in the late 1960s, has shown that it is possible to increase students' creative ability in the course of a degree program. Research into the identification of students who will benefit from the approach is continuing. (MSE)

  16. Young Children's Drawings in Problem Solving

    Science.gov (United States)

    Bakar, Kamariah Abu; Way, Jennifer; Bobis, Janette

    2016-01-01

    This paper explores young children's drawings (6 years old) in early number and addition activities in Malaysia. Observation, informal interviews and analysis of drawings revealed two types of drawing, and gave insight into the transitional process required for children to utilise drawings in problem solving. We argue the importance of valuing and…

  17. A method for solving neutron transport equation

    International Nuclear Information System (INIS)

    Dimitrijevic, Z.

    1993-01-01

    The procedure for solving the transport equation by directly integrating for case one-dimensional uniform multigroup medium is shown. The solution is expressed in terms of linear combination of function H n (x,μ), and the coefficient is determined from given conditions. The solution is applied for homogeneous slab of critical thickness. (author)

  18. On Teaching Problem Solving in School Mathematics

    Science.gov (United States)

    Pehkonen, Erkki; Näveri, Liisa; Laine, Anu

    2013-01-01

    The article begins with a brief overview of the situation throughout the world regarding problem solving. The activities of the ProMath group are then described, as the purpose of this international research group is to improve mathematics teaching in school. One mathematics teaching method that seems to be functioning in school is the use of open…

  19. Problem-Solving Test: Tryptophan Operon Mutants

    Science.gov (United States)

    Szeberenyi, Jozsef

    2010-01-01

    This paper presents a problem-solving test that deals with the regulation of the "trp" operon of "Escherichia coli." Two mutants of this operon are described: in mutant A, the operator region of the operon carries a point mutation so that it is unable to carry out its function; mutant B expresses a "trp" repressor protein unable to bind…

  20. A Microgenetic Study of Insightful Problem Solving

    Science.gov (United States)

    Luwel, Koen; Siegler, Robert S.; Verschaffel, Lieven

    2008-01-01

    An eight-session microgenetic study of acquisition of an insightful problem-solving strategy was conducted. A total of 35 second graders who did not use this insightful strategy initially were assigned to two groups that differed in the frequency of problems likely to facilitate discovery and generalization of the strategy. Children in the…

  1. Reinventing the Wheel: Design and Problem Solving

    Science.gov (United States)

    Blasetti, Sean M.

    2010-01-01

    This article describes a design problem that not only takes students through the technological design process, but it also provides them with real-world problem-solving experience as it relates to the manufacturing and engineering fields. It begins with a scenario placing the student as a custom wheel designer for an automotive manufacturing…

  2. Accounting Exams: Problem Solving or Multiple Choice.

    Science.gov (United States)

    Odaiyappa, Ramasamy

    1989-01-01

    The author studied whether students' preferences for a particular exam format were a function of past performances. He reviews the literature and reports on administration of a questionnaire to 90 accounting students. Results indicate that students prefer problem-solving exams to multiple choice, regardless of past performances. (CH)

  3. A tuning machine for cooperative problem solving

    NARCIS (Netherlands)

    Dunin-Keplicz, B; Verbrugge, R

    2004-01-01

    In this paper we aim to formally model individual, social and collective motivational attitudes in teams of agents involved in Cooperative Problem Solving. Particular attention is given to the strongest motivational attitude, collective commitment, which leads to team action. First, building on our

  4. Multimedia Application for Solving a Sudoku Game

    Directory of Open Access Journals (Sweden)

    Alasu Paul Sabrin

    2009-01-01

    Full Text Available This article explains the way in which, with the help of Action Script 3 in combination with Flash, a method of solving Sudoku game was implemented, through searching for the certain numbers and after that trying to guess for the squares where there are two possible numbers.

  5. Modeling visual problem solving as analogical reasoning.

    Science.gov (United States)

    Lovett, Andrew; Forbus, Kenneth

    2017-01-01

    We present a computational model of visual problem solving, designed to solve problems from the Raven's Progressive Matrices intelligence test. The model builds on the claim that analogical reasoning lies at the heart of visual problem solving, and intelligence more broadly. Images are compared via structure mapping, aligning the common relational structure in 2 images to identify commonalities and differences. These commonalities or differences can themselves be reified and used as the input for future comparisons. When images fail to align, the model dynamically rerepresents them to facilitate the comparison. In our analysis, we find that the model matches adult human performance on the Standard Progressive Matrices test, and that problems which are difficult for the model are also difficult for people. Furthermore, we show that model operations involving abstraction and rerepresentation are particularly difficult for people, suggesting that these operations may be critical for performing visual problem solving, and reasoning more generally, at the highest level. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  6. Solving Wicked Problems through Action Learning

    Science.gov (United States)

    Crul, Liselore

    2014-01-01

    This account of practice outlines the Oxyme Action Learning Program which was conducted as part of the Management Challenge in my final year of the MSc in Coaching and Behavioral Change at Henley Business School. The central research questions were: (1) how action learning can help to solve wicked problems and (2) what the effect of an action…

  7. Solving Mathematical Problems A Personal Perspective

    CERN Document Server

    Tao, Terence

    2006-01-01

    Authored by a leading name in mathematics, this engaging and clearly presented text leads the reader through the tactics involved in solving mathematical problems at the Mathematical Olympiad level. With numerous exercises and assuming only basic mathematics, this text is ideal for students of 14 years and above in pure mathematics.

  8. Artists Create Puzzles, Scientists Solve Them.

    Science.gov (United States)

    Goldstein, Joseph L

    2017-09-21

    The Spanish artist Diego Velázquez created a puzzle-painting 360 years ago that to this day remains unsolved, but still mystifies and intrigues. Unlike artists who get their thrills by creating puzzles that stimulate the imagination, scientists get their kicks by solving puzzles that advance biomedical research. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Raise the Bar on Problem Solving

    Science.gov (United States)

    Englard, Lisa

    2010-01-01

    In a 1981 diagnostic test, the Ministry of Education in Singapore found its country facing a challenge: Only 46 percent of students in grades 2-4 could solve word problems that were presented without such key words as "altogether" or "left." Yet today, according to results from the Trends in International Mathematics and…

  10. Adaptive wavelet algorithms for solving operator equations

    NARCIS (Netherlands)

    Gantumur, T.

    2006-01-01

    This thesis treats various aspects of adaptive wavelet algorithms for solving operator equations. For a separable Hilbert space H, a linear functional f in H', and a boundedly invertible linear operator A:H->H', we consider the problem of finding u from H satisfying Au=f. Typically A is given by a

  11. Brain dynamics of mathematical problem solving.

    Science.gov (United States)

    Lin, Chun-Ling; Jung, Melody; Wu, Ying Choon; Lin, Chin-Teng; She, Hsiao-Ching

    2012-01-01

    The purpose of this study is to examine brain activities of participants solving mental math problems. The research investigated how problem difficulty affected the subjects' responses and electroencephalogram (EEG) in different brain regions. In general, it was found that solution latencies (SL) to the math problems increased with difficulty. The EEG results showed that across subjects, the right-central beta, left-parietal theta, left-occipital theta and alpha, right-parietal alpha and beta, medial-frontal beta and medial central theta power decreased as task difficulty increased. This study further explored the effects of problem-solving performance on the EEG. Slow solvers exhibited greater frontal theta activities in the right hemisphere, whereas an inverse pattern of hemispheric asymmetry was found in fast solvers. Furthermore, analyses of spatio-temporal brain dynamics during problem solving show progressively stronger alpha- and beta-power suppression and theta-power augmentation as subjects were reaching a solution. These findings provide a better understanding of cortical activities mediating math-based problem solving and knowledge acquisition that can ultimately benefit math learning and education.

  12. Language and mathematical problem solving among bilinguals.

    Science.gov (United States)

    Bernardo, Allan B I

    2002-05-01

    Does using a bilingual's 1st or 2nd language have an effect on problem solving in semantically rich domains like school mathematics? The author conducted a study to determine whether Filipino-English bilingual students' understanding and solving of word problems in arithmetic differed when the problems were in the students' 1st and 2nd languages. Two groups participated-students whose 1st language was Filipino and students whose 1st language was English-and easy and difficult arithmetic problems were used. The author used a recall paradigm to assess how students understood the word problems and coded the solution accuracy to assess problem solving. The results indicated a 1st-language advantage; that is, the students were better able to understand and solve problems in their 1st language, whether the 1st language was English or Filipino. Moreover, the advantage was more marked with the easy problems. The theoretical and practical implications of the results are discussed.

  13. Discovering Steiner Triple Systems through Problem Solving

    Science.gov (United States)

    Sriraman, Bharath

    2004-01-01

    An attempt to implement problem solving as a teacher of ninth grade algebra is described. The problems selected were not general ones, they involved combinations and represented various situations and were more complex which lead to the discovery of Steiner triple systems.

  14. Counterfactual Problem Solving and Situated Cognition

    Directory of Open Access Journals (Sweden)

    Glebkin V.V.,

    2017-08-01

    Full Text Available The paper describes and interprets data of a study on counterfactual problem solving in representatives of modern industrial culture. The study was inspired by similar experiments carried out by A.R. Luria during his expedition to Central Asia. The hypothesis of our study was that representatives of modern industrial culture would solve counterfactual puzzles at a slower rate and with higher numbers of mistakes than similar non-counterfactual tasks. The experiments we conducted supported this hypothesis as well as provided us with some insights as to how to further develop it. For instance, we found no significant differences in time lag in solving counterfactual and ‘realistic’ tasks between the subjects with mathematical and the ones with liberal arts education. As an interpretation of the obtained data, we suggest a two-stage model of counterfactual problem solving: on the first stage, where situated cognition dominates, the realistic situation is transferred into the system of symbols unrelated to this very situation; on the second stage, operations are carried out within the framework of this new system of symbols.

  15. Fuzzy linear programming approach for solving transportation ...

    Indian Academy of Sciences (India)

    ALI EBRAHIMNEJAD

    satisfy demands at destinations using available supplies at origins. Transportation problem can be used for a wide vari- ety of situations such as scheduling, production, investment, plant location, inventory control, employment scheduling and many others. In general, transportation problems are solved with the assumptions ...

  16. Facilitating Problem Solving in High School Chemistry.

    Science.gov (United States)

    Gabel, Dorothy L.; Sherwood, Robert D.

    1983-01-01

    Investigated superiority of instructional strategies (factor-label method, proportionality, use of analogies, use of diagrams) in teaching problem-solving related to mole concept, gas laws, stoichiometry, and molarity. Also investigated effectiveness of strategies for students (N=609) with different verbal-visual preferences, proportional…

  17. Problem Solving. Workplace Strategies for Thoughtful Change.

    Science.gov (United States)

    Diller, Janelle; Moore, Rita

    This learning module is designed to enable participants to look at problems from a variety of perspectives, to apply a basic problem-solving strategy, to implement a plan of action, and to identify problems that are of particular importance to their workplace. The module includes units for six class sessions. Each unit includes the following…

  18. Problem Solving Model for Science Learning

    Science.gov (United States)

    Alberida, H.; Lufri; Festiyed; Barlian, E.

    2018-04-01

    This research aims to develop problem solving model for science learning in junior high school. The learning model was developed using the ADDIE model. An analysis phase includes curriculum analysis, analysis of students of SMP Kota Padang, analysis of SMP science teachers, learning analysis, as well as the literature review. The design phase includes product planning a science-learning problem-solving model, which consists of syntax, reaction principle, social system, support system, instructional impact and support. Implementation of problem-solving model in science learning to improve students' science process skills. The development stage consists of three steps: a) designing a prototype, b) performing a formative evaluation and c) a prototype revision. Implementation stage is done through a limited trial. A limited trial was conducted on 24 and 26 August 2015 in Class VII 2 SMPN 12 Padang. The evaluation phase was conducted in the form of experiments at SMPN 1 Padang, SMPN 12 Padang and SMP National Padang. Based on the development research done, the syntax model problem solving for science learning at junior high school consists of the introduction, observation, initial problems, data collection, data organization, data analysis/generalization, and communicating.

  19. Nanomedicine: Problem Solving to Treat Cancer

    Science.gov (United States)

    Hemling, Melissa A.; Sammel, Lauren M.; Zenner, Greta; Payne, Amy C.; Crone, Wendy C.

    2006-01-01

    Many traditional classroom science and technology activities often ask students to complete prepackaged labs that ensure that everyone arrives at the same "scientifically accurate" solution or theory, which ignores the important problem-solving and creative aspects of scientific research and technological design. Students rarely have the…

  20. USING SYSTEMIC PROBLEM SOLVING (SPS) TO ASSESS ...

    African Journals Online (AJOL)

    unesco

    Also, systemic problem solving (SPS) helps students to connect chemistry concepts, and facts and covers a wide range of ... So, by using SPS we assess the student achievement in three systemic levels of learning chemistry: the macro ..... Johnstone A.H. (2000). Teaching of Chemistry – logical or psychological? CERAPIE ...

  1. Combined Approach for Solving the Electromagnetic Induction ...

    African Journals Online (AJOL)

    Nafiisah

    The entries in vector Z. ∆ are, in general, complex numbers. In a practical situation, the material distribution in the object space is unknown, and the inverse problem (or image reconstruction problem) consists in estimating it from the measurable vector Z. ∆ . Solving the general inverse problem presents a formidable difficulty ...

  2. Evolution of mal ABC transporter operons in the Thermococcales and Thermotogales

    Directory of Open Access Journals (Sweden)

    Gogarten J Peter

    2008-01-01

    Full Text Available Abstract Background The mal genes that encode maltose transporters have undergone extensive lateral transfer among ancestors of the archaea Thermococcus litoralis and Pyrococcus furiosus. Bacterial hyperthermophiles of the order Thermotogales live among these archaea and so may have shared in these transfers. The genome sequence of Thermotoga maritima bears evidence of extensive acquisition of archaeal genes, so its ancestors clearly had the capacity to do so. We examined deep phylogenetic relationships among the mal genes of these hyperthermophiles and their close relatives to look for evidence of shared ancestry. Results We demonstrate that the two maltose ATP binding cassette (ABC transporter operons now found in Tc. litoralis and P. furiosus (termed mal and mdx genes, respectively are not closely related to one another. The Tc. litoralis and P. furiosus mal genes are most closely related to bacterial mal genes while their respective mdx genes are archaeal. The genes of the two mal operons in Tt. maritima are not related to genes in either of these archaeal operons. They are highly similar to one another and belong to a phylogenetic lineage that includes mal genes from the enteric bacteria. A unique domain of the enteric MalF membrane spanning proteins found also in these Thermotogales MalF homologs supports their relatively close relationship with these enteric proteins. Analyses of genome sequence data from other Thermotogales species, Fervidobacterium nodosum, Thermosipho melanesiensis, Thermotoga petrophila, Thermotoga lettingae, and Thermotoga neapolitana, revealed a third apparent mal operon, absent from the published genome sequence of Tt. maritima strain MSB8. This third operon, mal3, is more closely related to the Thermococcales' bacteria-derived mal genes than are mal1 and mal2. F. nodosum, Ts. melanesiensis, and Tt. lettingae have only one of the mal1-mal2 paralogs. The mal2 operon from an unknown species of Thermotoga appears to

  3. Mathematical Problem Solving: A Review of the Literature.

    Science.gov (United States)

    Funkhouser, Charles

    The major perspectives on problem solving of the twentieth century are reviewed--associationism, Gestalt psychology, and cognitive science. The results of the review on teaching problem solving and the uses of computers to teach problem solving are included. Four major issues related to the teaching of problem solving are discussed: (1)…

  4. Young Children's Analogical Problem Solving: Gaining Insights from Video Displays

    Science.gov (United States)

    Chen, Zhe; Siegler, Robert S.

    2013-01-01

    This study examined how toddlers gain insights from source video displays and use the insights to solve analogous problems. Two- to 2.5-year-olds viewed a source video illustrating a problem-solving strategy and then attempted to solve analogous problems. Older but not younger toddlers extracted the problem-solving strategy depicted in the video…

  5. Learning Matlab a problem solving approach

    CERN Document Server

    Gander, Walter

    2015-01-01

    This comprehensive and stimulating introduction to Matlab, a computer language now widely used for technical computing, is based on an introductory course held at Qian Weichang College, Shanghai University, in the fall of 2014.  Teaching and learning a substantial programming language aren’t always straightforward tasks. Accordingly, this textbook is not meant to cover the whole range of this high-performance technical programming environment, but to motivate first- and second-year undergraduate students in mathematics and computer science to learn Matlab by studying representative problems, developing algorithms and programming them in Matlab. While several topics are taken from the field of scientific computing, the main emphasis is on programming. A wealth of examples are completely discussed and solved, allowing students to learn Matlab by doing: by solving problems, comparing approaches and assessing the proposed solutions.

  6. What is physics problem solving competency?

    DEFF Research Database (Denmark)

    Niss, Martin

    2018-01-01

    on the nature of physics problem- solving competency. The first, Sommerfeld’s, is a “theory first, phenomenon second” approach. Here the relevant problems originate in one of the theories of physics and the job goal of the problem- solver is to make a mathematical analysis of the suitable equation......A central goal of physics education is to teach problem-solving competency, but the nature of this competency is not well-described in the literature. The present paperarticle uses recent historical scholarship on Arnold Sommerfeld and Enrico Fermi to identify and characterize two positions......(s) and then give a qualitative analysis of the phenomenon that arise from these mathematical results. Fermi’s position is a “phenomenon first, theory second” approach, where the starting point is a physical phenomenon that is analyzed and then brought into the realm of a physics theory. The two positions...

  7. Creative problem solving: an applied university course

    Directory of Open Access Journals (Sweden)

    René Victor Valqui Vidal

    2010-08-01

    Full Text Available This paper presents the principles of active learning and the contents of a creativity course entitled: Creativity and Problem Solving. The main purpose of this course is to create a space to discuss, reflect and experiment with creativity, creative processes and creative tools of relevance for students of any speciality (60% will end as operational researchers working with problem solving approaches. This course has run with big success since 1998 at the Technical University of Denmark. It started with very few students, now is a very popular course attracting many students from abroad. The selected themes, the methods and techniques, the structure of this course, the learning processes and the achieved results are presented. The results of student's and teacher's evaluations are also outlined. Finally some reflections, recommendations and conclusions are discussed.

  8. Solving ptychography with a convex relaxation

    Science.gov (United States)

    Horstmeyer, Roarke; Chen, Richard Y.; Ou, Xiaoze; Ames, Brendan; Tropp, Joel A.; Yang, Changhuei

    2015-05-01

    Ptychography is a powerful computational imaging technique that transforms a collection of low-resolution images into a high-resolution sample reconstruction. Unfortunately, algorithms that currently solve this reconstruction problem lack stability, robustness, and theoretical guarantees. Recently, convex optimization algorithms have improved the accuracy and reliability of several related reconstruction efforts. This paper proposes a convex formulation of the ptychography problem. This formulation has no local minima, it can be solved using a wide range of algorithms, it can incorporate appropriate noise models, and it can include multiple a priori constraints. The paper considers a specific algorithm, based on low-rank factorization, whose runtime and memory usage are near-linear in the size of the output image. Experiments demonstrate that this approach offers a 25% lower background variance on average than alternating projections, the ptychographic reconstruction algorithm that is currently in widespread use.

  9. Students’ Covariational Reasoning in Solving Integrals’ Problems

    Science.gov (United States)

    Harini, N. V.; Fuad, Y.; Ekawati, R.

    2018-01-01

    Covariational reasoning plays an important role to indicate quantities vary in learning calculus. This study investigates students’ covariational reasoning during their studies concerning two covarying quantities in integral problem. Six undergraduate students were chosen to solve problems that involved interpreting and representing how quantities change in tandem. Interviews were conducted to reveal the students’ reasoning while solving covariational problems. The result emphasizes that undergraduate students were able to construct the relation of dependent variables that changes in tandem with the independent variable. However, students faced difficulty in forming images of continuously changing rates and could not accurately apply the concept of integrals. These findings suggest that learning calculus should be increased emphasis on coordinating images of two quantities changing in tandem about instantaneously rate of change and to promote conceptual knowledge in integral techniques.

  10. Physics solving-problem mediated by technology

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Souza

    2008-08-01

    Full Text Available We questioned the traditional practice of Physics teaching interrogating the normal procedures of solving-problem. We pointed the students passivity out, the lack of dialog interaction, the teaching-learning activity isolated from investigation, as responsible for the technological-communication non-mediation in classroom and, consequently, the lack of the problem-dialog in school process. Oriented by the investigation-action conceptions and by the problem-dialog education, we developed, established and evaluated an school object for solving-problem linked in to a teaching-learning virtual environment for the Internet, both with open sources, prioritizing empowered didactic strategies of procedures and abilities which we named as teachinginvestigation- learning in a problem perspective

  11. Multiscale empirical interpolation for solving nonlinear PDEs

    KAUST Repository

    Calo, Victor M.

    2014-12-01

    In this paper, we propose a multiscale empirical interpolation method for solving nonlinear multiscale partial differential equations. The proposed method combines empirical interpolation techniques and local multiscale methods, such as the Generalized Multiscale Finite Element Method (GMsFEM). To solve nonlinear equations, the GMsFEM is used to represent the solution on a coarse grid with multiscale basis functions computed offline. Computing the GMsFEM solution involves calculating the system residuals and Jacobians on the fine grid. We use empirical interpolation concepts to evaluate these residuals and Jacobians of the multiscale system with a computational cost which is proportional to the size of the coarse-scale problem rather than the fully-resolved fine scale one. The empirical interpolation method uses basis functions which are built by sampling the nonlinear function we want to approximate a limited number of times. The coefficients needed for this approximation are computed in the offline stage by inverting an inexpensive linear system. The proposed multiscale empirical interpolation techniques: (1) divide computing the nonlinear function into coarse regions; (2) evaluate contributions of nonlinear functions in each coarse region taking advantage of a reduced-order representation of the solution; and (3) introduce multiscale proper-orthogonal-decomposition techniques to find appropriate interpolation vectors. We demonstrate the effectiveness of the proposed methods on several nonlinear multiscale PDEs that are solved with Newton\\'s methods and fully-implicit time marching schemes. Our numerical results show that the proposed methods provide a robust framework for solving nonlinear multiscale PDEs on a coarse grid with bounded error and significant computational cost reduction.

  12. Ukraine's Participation In Solving Climate Change Problems

    OpenAIRE

    Irina Dubovich; Mariana Bulgakova

    2011-01-01

    Attention is paid to some problems of climate change. The main international agreements on climate change are overviewed. Ukraine's participation in solving global problems of climate change is described. Ukraine's statement about plans to reduce greenhouse gas emissions is analyzed. Characteristic of environmental political and legal prerequisites for the need to create a general agreement on environmental security of the planet – World Environmental Constitution is provided.

  13. Rational approximatons for solving cauchy problems

    Directory of Open Access Journals (Sweden)

    Veyis Turut

    2016-08-01

    Full Text Available In this letter, numerical solutions of Cauchy problems are considered by multivariate Padé approximations (MPA. Multivariate Padé approximations (MPA were applied to power series solutions of Cauchy problems that solved by using He’s variational iteration method (VIM. Then, numerical results obtained by using multivariate Padé approximations were compared with the exact solutions of Cauchy problems.

  14. Solving Nonlinear Euler Equations with Arbitrary Accuracy

    Science.gov (United States)

    Dyson, Rodger W.

    2005-01-01

    A computer program that efficiently solves the time-dependent, nonlinear Euler equations in two dimensions to an arbitrarily high order of accuracy has been developed. The program implements a modified form of a prior arbitrary- accuracy simulation algorithm that is a member of the class of algorithms known in the art as modified expansion solution approximation (MESA) schemes. Whereas millions of lines of code were needed to implement the prior MESA algorithm, it is possible to implement the present MESA algorithm by use of one or a few pages of Fortran code, the exact amount depending on the specific application. The ability to solve the Euler equations to arbitrarily high accuracy is especially beneficial in simulations of aeroacoustic effects in settings in which fully nonlinear behavior is expected - for example, at stagnation points of fan blades, where linearizing assumptions break down. At these locations, it is necessary to solve the full nonlinear Euler equations, and inasmuch as the acoustical energy is of the order of 4 to 5 orders of magnitude below that of the mean flow, it is necessary to achieve an overall fractional error of less than 10-6 in order to faithfully simulate entropy, vortical, and acoustical waves.

  15. Gender differences in advanced mathematical problem solving.

    Science.gov (United States)

    Gallagher, A M; De Lisi, R; Holst, P C; McGillicuddy-De Lisi, A V; Morely, M; Cahalan, C

    2000-03-01

    Strategy flexibility in mathematical problem solving was investigated. In Studies 1 and 2, high school juniors and seniors solved Scholastic Assessment Test-Mathematics (SAT-M) problems classified as conventional or unconventional. Algorithmic solution strategies were students' default choice for both types of problems across conditions that manipulated item format and solution time. Use of intuitive strategies on unconventional problems was evident only for high-ability students. Male students were more likely than female students to successfully match strategies to problem characteristics. In Study 3, a revised taxonomy of problems based on cognitive solution demands was predictive of gender differences on Graduate Record Examination-Quantitative (GRE-Q) items. Men outperformed women overall, but the difference was greater on items requiring spatial skills, shortcuts, or multiple solution paths than on problems requiring verbal skills or mastery of classroom-based content. Results suggest that strategy flexibility is a source of gender differences in mathematical ability assessed by SAT-M and GRE-Q problem solving. Copyright 2000 Academic Press.

  16. Characteristics of students in comparative problem solving

    Science.gov (United States)

    Irfan, M.; Sudirman; Rahardi, R.

    2018-01-01

    Often teachers provided examples and exercised to students with regard to comparative problems consisting of one quantity. In this study, the researchers gave the problem of comparison with the two quantities mixed. It was necessary to have a good understanding to solve this problem. This study aimed to determine whether students understand the comparison in depth and be able to solve the problem of non-routine comparison. This study used qualitative explorative methods, with researchers conducting in-depth interviews on subjects to explore the thinking process when solving comparative problems. The subject of this study was three students selected by purposive sampling of 120 students. From this research, researchers found there were three subjects with different characteristics, namely: subject 1, he did the first and second questions with methods of elimination and substitution (non-comparison); subject 2, he did the first question with the concept of comparison although the answer was wrong, and did the second question with the method of elimination and substitution (non-comparison); and subject 3, he did both questions with the concept of comparison. In the first question, he did wrong because he was unable to understand the problem, while on the second he did correctly. From the characteristics of the answers, the researchers divided into 3 groups based on thinking process, namely: blind-proportion, partial-proportion, and proportion thinking.

  17. Learning via problem solving in mathematics education

    Directory of Open Access Journals (Sweden)

    Piet Human

    2009-09-01

    Full Text Available Three forms of mathematics education at school level are distinguished: direct expository teaching with an emphasis on procedures, with the expectation that learners will at some later stage make logical and functional sense of what they have learnt and practised (the prevalent form, mathematically rigorous teaching in terms of fundamental mathematical concepts, as in the so-called “modern mathematics” programmes of the sixties, teaching and learning in the context of engaging with meaningful problems and focused both on learning to become good problem solvers (teaching for problem solving andutilising problems as vehicles for the development of mathematical knowledge andproficiency by learners (problem-centred learning, in conjunction with substantialteacher-led social interaction and mathematical discourse in classrooms.Direct expository teaching of mathematical procedures dominated in school systems after World War II, and was augmented by the “modern mathematics” movement in the period 1960-1970. The latter was experienced as a major failure, and was soon abandoned. Persistent poor outcomes of direct expository procedural teaching of mathematics for the majority of learners, as are still being experienced in South Africa, triggered a world-wide movement promoting teaching mathematics for and via problem solving in the seventies and eighties of the previous century. This movement took the form of a variety of curriculum experiments in which problem solving was the dominant classroom activity, mainly in the USA, Netherlands, France and South Africa. While initially focusing on basic arithmetic (computation with whole numbers and elementary calculus, the problem-solving movement started to address other mathematical topics (for example, elementary statistics, algebra, differential equations around the turn of the century. The movement also spread rapidly to other countries, including Japan, Singapore and Australia. Parallel with the

  18. Processes involved in solving mathematical problems

    Science.gov (United States)

    Shahrill, Masitah; Putri, Ratu Ilma Indra; Zulkardi, Prahmana, Rully Charitas Indra

    2018-04-01

    This study examines one of the instructional practices features utilized within the Year 8 mathematics lessons in Brunei Darussalam. The codes from the TIMSS 1999 Video Study were applied and strictly followed, and from the 183 mathematics problems recorded, there were 95 problems with a solution presented during the public segments of the video-recorded lesson sequences of the four sampled teachers. The analyses involved firstly, identifying the processes related to mathematical problem statements, and secondly, examining the different processes used in solving the mathematical problems for each problem publicly completed during the lessons. The findings revealed that for three of the teachers, their problem statements coded as `using procedures' ranged from 64% to 83%, while the remaining teacher had 40% of his problem statements coded as `making connections.' The processes used when solving the problems were mainly `using procedures', and none of the problems were coded as `giving results only'. Furthermore, all four teachers made use of making the relevant connections in solving the problems given to their respective students.

  19. Students’ difficulties in solving linear equation problems

    Science.gov (United States)

    Wati, S.; Fitriana, L.; Mardiyana

    2018-03-01

    A linear equation is an algebra material that exists in junior high school to university. It is a very important material for students in order to learn more advanced mathematics topics. Therefore, linear equation material is essential to be mastered. However, the result of 2016 national examination in Indonesia showed that students’ achievement in solving linear equation problem was low. This fact became a background to investigate students’ difficulties in solving linear equation problems. This study used qualitative descriptive method. An individual written test on linear equation tasks was administered, followed by interviews. Twenty-one sample students of grade VIII of SMPIT Insan Kamil Karanganyar did the written test, and 6 of them were interviewed afterward. The result showed that students with high mathematics achievement donot have difficulties, students with medium mathematics achievement have factual difficulties, and students with low mathematics achievement have factual, conceptual, operational, and principle difficulties. Based on the result there is a need of meaningfulness teaching strategy to help students to overcome difficulties in solving linear equation problems.

  20. The Impact of Teacher Training on Creative Writing and Problem-Solving Using Futuristic Scenarios for Creative Problem Solving and Creative Problem Solving Programs

    Science.gov (United States)

    Hayel Al-Srour, Nadia; Al-Ali, Safa M.; Al-Oweidi, Alia

    2016-01-01

    The present study aims to detect the impact of teacher training on creative writing and problem-solving using both Futuristic scenarios program to solve problems creatively, and creative problem solving. To achieve the objectives of the study, the sample was divided into two groups, the first consist of 20 teachers, and 23 teachers to second…

  1. Effects of the SOLVE Strategy on the Mathematical Problem Solving Skills of Secondary Students with Learning Disabilities

    Science.gov (United States)

    Freeman-Green, Shaqwana M.; O'Brien, Chris; Wood, Charles L.; Hitt, Sara Beth

    2015-01-01

    This study examined the effects of explicit instruction in the SOLVE Strategy on the mathematical problem solving skills of six Grade 8 students with specific learning disabilities. The SOLVE Strategy is an explicit instruction, mnemonic-based learning strategy designed to help students in solving mathematical word problems. Using a multiple probe…

  2. Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense.

    Science.gov (United States)

    Kluskens, L D; Zeilstra, J; Geerling, A C M; de Vos, W M; van der Oost, J

    2010-09-01

    The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized. It is optimally active at 70 degrees C, pH 7.3, with a specific activity of 15.0 U/mg for the interconversion of glucose to fructose. When compared with T. maritima XylA at 85 degrees C, a higher catalytic efficiency was observed. Divalent metal ions Co2+ and Mg2+ were found to enhance the thermostability.

  3. Neural bases for basic processes in heuristic problem solving: Take solving Sudoku puzzles as an example.

    Science.gov (United States)

    Qin, Yulin; Xiang, Jie; Wang, Rifeng; Zhou, Haiyan; Li, Kuncheng; Zhong, Ning

    2012-12-01

    Newell and Simon postulated that the basic steps in human problem-solving involve iteratively applying operators to transform the state of the problem to eventually achieve a goal. To check the neural basis of this framework, the present study focused on the basic processes in human heuristic problem-solving that the participants identified the current problem state and then recalled and applied the corresponding heuristic rules to change the problem state. A new paradigm, solving simplified Sudoku puzzles, was developed for an event-related functional magnetic resonance imaging (fMRI) study in problem solving. Regions of interest (ROIs), including the left prefrontal cortex, the bilateral posterior parietal cortex, the anterior cingulated cortex, the bilateral caudate nuclei, the bilateral fusiform, as well as the bilateral frontal eye fields, were found to be involved in the task. To obtain convergent evidence, in addition to traditional statistical analysis, we used the multivariate voxel classification method to check the accuracy of the predictions for the condition of the task from the blood oxygen level dependent (BOLD) response of the ROIs, using a new classifier developed in this study for fMRI data. To reveal the roles that the ROIs play in problem solving, we developed an ACT-R computational model of the information-processing processes in human problem solving, and tried to predict the BOLD response of the ROIs from the task. Advances in human problem-solving research after Newell and Simon are then briefly discussed. © 2012 The Institute of Psychology, Chinese Academy of Sciences and Blackwell Publishing Asia Pty Ltd.

  4. Individual differences in solving arithmetic word problems

    Science.gov (United States)

    2013-01-01

    Background With the present functional magnetic resonance imaging (fMRI) study at 3 T, we investigated the neural correlates of visualization and verbalization during arithmetic word problem solving. In the domain of arithmetic, visualization might mean to visualize numbers and (intermediate) results while calculating, and verbalization might mean that numbers and (intermediate) results are verbally repeated during calculation. If the brain areas involved in number processing are domain-specific as assumed, that is, that the left angular gyrus (AG) shows an affinity to the verbal domain, and that the left and right intraparietal sulcus (IPS) shows an affinity to the visual domain, the activation of these areas should show a dependency on an individual’s cognitive style. Methods 36 healthy young adults participated in the fMRI study. The participants habitual use of visualization and verbalization during solving arithmetic word problems was assessed with a short self-report assessment. During the fMRI measurement, arithmetic word problems that had to be solved by the participants were presented in an event-related design. Results We found that visualizers showed greater brain activation in brain areas involved in visual processing, and that verbalizers showed greater brain activation within the left angular gyrus. Conclusions Our results indicate that cognitive styles or preferences play an important role in understanding brain activation. Our results confirm, that strong visualizers use mental imagery more strongly than weak visualizers during calculation. Moreover, our results suggest that the left AG shows a specific affinity to the verbal domain and subserves number processing in a modality-specific way. PMID:23883107

  5. SOLVING GLOBAL PROBLEMS USING COLLABORATIVE DESIGN PROCESSES

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Mejborn, Christina Okai

    2011-01-01

    In this paper we argue that use of collaborative design processes is a powerful means of bringing together different stakeholders and generating ideas in complex design situations. The collaborative design process was used in a workshop with international participants where the goal was to propose...... forward proposed solutions for how to design, brand and make business models for how to solve aspects of the sanitation problem. The workshop showed that it was possible to work freely with such a taboo topic and that in particular the use of visualisation tools, i.e. drawing posters and building simple...

  6. SOLVING GLOBAL PROBLEMS USING COLLABORATIVE DESIGN PROCESSES

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Mejborn, Christina Okai

    2011-01-01

    In this paper we argue that use of collaborative design processes is a powerful means of bringing together different stakeholders and generating ideas in complex design situations. The collaborative design process was used in a workshop with international participants where the goal was to propose...... new solutions that would help solve the global problem of sanitation. Lack of sanitation is a problem for 42% of the world’s population but it is also a taboo topic that only very few people will engage in. In the one-day workshop participants from very different areas came together and brought...

  7. Programming languages for business problem solving

    CERN Document Server

    Wang, Shouhong

    2007-01-01

    It has become crucial for managers to be computer literate in today's business environment. It is also important that those entering the field acquire the fundamental theories of information systems, the essential practical skills in computer applications, and the desire for life-long learning in information technology. Programming Languages for Business Problem Solving presents a working knowledge of the major programming languages, including COBOL, C++, Java, HTML, JavaScript, VB.NET, VBA, ASP.NET, Perl, PHP, XML, and SQL, used in the current business computing environment. The book examin

  8. Spectral difference methods for solving differential equations

    Science.gov (United States)

    Mazziotti, David A.

    1999-01-01

    A family of recently developed techniques is explored for achieving both matrix sparsity and rapid convergence when numerically solving differential and eigenvalue equations without domain decomposition. These methods, which we call spectral differences, include Boyd's sum acceleration techniques and the Lagrange distributed approximating functional (LDAF) approach. A formula is developed for estimating the unknown Gaussian parameter within LDAF. We implement these methods to calculate the Morse vibrational energies for diatomic iodine. For equivalent bandwidths the sum acceleration with finite difference weights generates energies which are between two and three orders of magnitude more accurate than those from LDAF.

  9. Solving jigsaw puzzles using image features

    DEFF Research Database (Denmark)

    Nielsen, Ture R.; Drewsen, Peter; Hansen, Klaus

    2008-01-01

    In this article, we describe a method for automatic solving of the jigsaw puzzle problem based on using image features instead of the shape of the pieces. The image features are used for obtaining an accurate measure for edge similarity to be used in a new edge matching algorithm. The algorithm...... algorithm which exploits the divide and conquer paradigm to reduce the combinatorially complex problem by classifying the puzzle pieces and comparing pieces drawn from the same group. The paper includes a brief preliminary investigation of some image features used in the classification....

  10. Solving crystal structures from neutron diffraction data

    International Nuclear Information System (INIS)

    Wilson, C.C.

    1987-07-01

    In order to pursue crystal structure determination using neutron diffraction data, and given the wide experience available of solving structures using X-ray data, the codes used in X-ray structural analysis should be adapted to the different requirements of a neutron experiment. Modifications have been made to a direct methods program MITHRIL and to a Patterson methods program PATMET to incorporate into these the features of neutron rather than X-ray diffraction. While to date these modifications have been fairly straightforward and many sophistications remain to be exploited, results obtained from the neutron versions of both programs are promising. (author)

  11. Comprehension and computation in Bayesian problem solving

    Directory of Open Access Journals (Sweden)

    Eric D. Johnson

    2015-07-01

    Full Text Available Humans have long been characterized as poor probabilistic reasoners when presented with explicit numerical information. Bayesian word problems provide a well-known example of this, where even highly educated and cognitively skilled individuals fail to adhere to mathematical norms. It is widely agreed that natural frequencies can facilitate Bayesian reasoning relative to normalized formats (e.g. probabilities, percentages, both by clarifying logical set-subset relations and by simplifying numerical calculations. Nevertheless, between-study performance on transparent Bayesian problems varies widely, and generally remains rather unimpressive. We suggest there has been an over-focus on this representational facilitator (i.e. transparent problem structures at the expense of the specific logical and numerical processing requirements and the corresponding individual abilities and skills necessary for providing Bayesian-like output given specific verbal and numerical input. We further suggest that understanding this task-individual pair could benefit from considerations from the literature on mathematical cognition, which emphasizes text comprehension and problem solving, along with contributions of online executive working memory, metacognitive regulation, and relevant stored knowledge and skills. We conclude by offering avenues for future research aimed at identifying the stages in problem solving at which correct versus incorrect reasoners depart, and how individual difference might influence this time point.

  12. Back to Basics: Solving Games with SAT

    Directory of Open Access Journals (Sweden)

    QUER, S.

    2016-08-01

    Full Text Available Games became popular, within the formal verification community, after their application to automatic synthesis of circuits from specifications, and they have been receiving more and more attention since then. This paper focuses on coding the "Sokoban" puzzle, i.e., a very complex single-player strategy game. We show how its solution can be encoded and represented as a Bounded Model Checking problem, and then solved with a SAT solver. After that, to cope with very complex instances of the game, we propose two different ad-hoc divide-and-conquer strategies. Those strategies, somehow similar to state-of-the-art abstraction-and-refinement schemes, are able to decompose deep Bounded Model Checking instances into easier subtasks, trading-off between efficiency and completeness. We analyze a vast set of difficult hard-to-solve benchmark games, trying to push forward the applicability of state-of-the-art SAT solvers in the field. Those results show that games may provide one of the next frontier for the SAT community.

  13. A Flipped Pedagogy for Expert Problem Solving

    Science.gov (United States)

    Pritchard, David

    The internet provides free learning opportunities for declarative (Wikipedia, YouTube) and procedural (Kahn Academy, MOOCs) knowledge, challenging colleges to provide learning at a higher cognitive level. Our ``Modeling Applied to Problem Solving'' pedagogy for Newtonian Mechanics imparts strategic knowledge - how to systematically determine which concepts to apply and why. Declarative and procedural knowledge is learned online before class via an e-text, checkpoint questions, and homework on edX.org (see http://relate.mit.edu/physicscourse); it is organized into five Core Models. Instructors then coach students on simple ``touchstone problems'', novel exercises, and multi-concept problems - meanwhile exercising three of the four C's: communication, collaboration, critical thinking and problem solving. Students showed 1.2 standard deviations improvement on the MIT final exam after three weeks instruction, a significant positive shift in 7 of the 9 categories in the CLASS, and their grades improved by 0.5 standard deviation in their following physics course (Electricity and Magnetism).

  14. Neural correlates of mathematical problem solving.

    Science.gov (United States)

    Lin, Chun-Ling; Jung, Melody; Wu, Ying Choon; She, Hsiao-Ching; Jung, Tzyy-Ping

    2015-03-01

    This study explores electroencephalography (EEG) brain dynamics associated with mathematical problem solving. EEG and solution latencies (SLs) were recorded as 11 neurologically healthy volunteers worked on intellectually challenging math puzzles that involved combining four single-digit numbers through basic arithmetic operators (addition, subtraction, division, multiplication) to create an arithmetic expression equaling 24. Estimates of EEG spectral power were computed in three frequency bands - θ (4-7 Hz), α (8-13 Hz) and β (14-30 Hz) - over a widely distributed montage of scalp electrode sites. The magnitude of power estimates was found to change in a linear fashion with SLs - that is, relative to a base of power spectrum, theta power increased with longer SLs, while alpha and beta power tended to decrease. Further, the topographic distribution of spectral fluctuations was characterized by more pronounced asymmetries along the left-right and anterior-posterior axes for solutions that involved a longer search phase. These findings reveal for the first time the topography and dynamics of EEG spectral activities important for sustained solution search during arithmetical problem solving.

  15. Using qualitative problem-solving strategies to highlight the role of conceptual knowledge in solving problems

    Science.gov (United States)

    Leonard, William J.; Dufresne, Robert J.; Mestre, Jose P.

    1996-12-01

    We report on the use of qualitative problem-solving strategies in teaching an introductory, calculus-based physics course as a means of highlighting the role played by conceptual knowledge in solving problems. We found that presenting strategies during lectures and in homework solutions provides an excellent opportunity to model for students the type of concept-based, qualitative reasoning that is valued in our profession, and that student-generated strategies serve a diagnostic function by providing instructors with insights on students' conceptual understanding and reasoning. Finally, we found strategies to be effective pedagogical tools for helping students both to identify principles that could be applied to solve specific problems, as well as to recall the major principles covered in the course months after it was over.

  16. Spontaneous gestures influence strategy choices in problem solving.

    Science.gov (United States)

    Alibali, Martha W; Spencer, Robert C; Knox, Lucy; Kita, Sotaro

    2011-09-01

    Do gestures merely reflect problem-solving processes, or do they play a functional role in problem solving? We hypothesized that gestures highlight and structure perceptual-motor information, and thereby make such information more likely to be used in problem solving. Participants in two experiments solved problems requiring the prediction of gear movement, either with gesture allowed or with gesture prohibited. Such problems can be correctly solved using either a perceptual-motor strategy (simulation of gear movements) or an abstract strategy (the parity strategy). Participants in the gesture-allowed condition were more likely to use perceptual-motor strategies than were participants in the gesture-prohibited condition. Gesture promoted use of perceptual-motor strategies both for participants who talked aloud while solving the problems (Experiment 1) and for participants who solved the problems silently (Experiment 2). Thus, spontaneous gestures influence strategy choices in problem solving.

  17. Applying FOCUS-PDCA to solve clinical problems.

    Science.gov (United States)

    Redick, E L

    1999-01-01

    Many methods have been proposed for solving complex or multifaceted clinical problems in a logical, systematic, and accurate way. This article describes one method of step-by-step problem solving and how to apply it to a clinical situation.

  18. The direct algorithm for solving of the graph isomorphism problem

    OpenAIRE

    Faizullin, Rashit T.; Prolubnikov, Alexander V.

    2005-01-01

    We propose an algorithm for solving of the graph isomorphism problem. Also, we introduce the new class of graphs for which the graph isomorphism problem can be solved polynomially using the algorithm.

  19. A Decision Support System for Solving Multiple Criteria Optimization Problems

    Science.gov (United States)

    Filatovas, Ernestas; Kurasova, Olga

    2011-01-01

    In this paper, multiple criteria optimization has been investigated. A new decision support system (DSS) has been developed for interactive solving of multiple criteria optimization problems (MOPs). The weighted-sum (WS) approach is implemented to solve the MOPs. The MOPs are solved by selecting different weight coefficient values for the criteria…

  20. Teaching Young Children Interpersonal Problem-Solving Skills

    Science.gov (United States)

    Joseph, Gail E.; Strain, Phillip S.

    2010-01-01

    Learning how to problem solve is one of the key developmental milestones in early childhood. Children's problem-solving skills represent a key feature in the development of social competence. Problem solving allows children to stay calm during difficult situations, repair social relations quickly, and get their needs met in ways that are safe and…

  1. Tracer Evolution at High Latitudes in Winter (SOLVE)

    Science.gov (United States)

    Plumb, R. Alan

    2001-01-01

    Prior to the SOLVE deployment, we modified and ran a chemical transport model (CTM) to set up a stratospheric climatology for 1 September 1999. During and immediately following the SOLVE deployments, we ran the model in near-real-time and used these results in conjunction with SOLVE observations to draw conclusions about stratospheric transport.

  2. Translation among Symbolic Representations in Problem-Solving. Revised.

    Science.gov (United States)

    Shavelson, Richard J.; And Others

    This study investigated the relationships among the symbolic representation of problems given to students to solve, the mental representations they use to solve the problems, and the accuracy of their solutions. Twenty eleventh-grade science students were asked to think aloud as they solved problems on the ideal gas laws. The problems were…

  3. Solving the minimum flow problem with interval bounds and flows

    Indian Academy of Sciences (India)

    and Ciurea et al (2008b) solved the minimum flow problem for bipartite networks, and Ciurea. & Deaconu (2007) solved the ... In Ghiyasvand (2011), a new method to solve the minimum cost flow problem with interval .... multiplication of convex sets, when the fuzzification uses Definition 1 for max–min and order operations.

  4. The Influence of Cognitive Abilities on Mathematical Problem Solving Performance

    Science.gov (United States)

    Bahar, Abdulkadir

    2013-01-01

    Problem solving has been a core theme in education for several decades. Educators and policy makers agree on the importance of the role of problem solving skills for school and real life success. A primary purpose of this study was to investigate the influence of cognitive abilities on mathematical problem solving performance of students. The…

  5. The Design Process for "PLATO[R] Math Problem Solving."

    Science.gov (United States)

    Mulcahy, Robert

    2001-01-01

    PLATO Learning, Inc., a developer of computer-based instruction, recently released "Math Problem Solving." This product was designed to teach strategies for solving math problems, and consists of 19 problem-solving activities, ranging from basic math to algebra. Each activity includes tools to help find a solution and rule-based coaching to…

  6. Thinking Process of Naive Problem Solvers to Solve Mathematical Problems

    Science.gov (United States)

    Mairing, Jackson Pasini

    2017-01-01

    Solving problems is not only a goal of mathematical learning. Students acquire ways of thinking, habits of persistence and curiosity, and confidence in unfamiliar situations by learning to solve problems. In fact, there were students who had difficulty in solving problems. The students were naive problem solvers. This research aimed to describe…

  7. AcEST: DK952773 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0Y1|P1254_THEMA Phosphorylated carbohydrates phosphatase T... 58 4e-08 sp|P77247|YNIC_ECOLI Phosphatase yniC...hydrates phosphatase TM_1254 OS=Thermotoga maritima GN=TM_1254 PE=1 SV=1 Length = 2...PELYLLAAKNLGVSPAECLAFEDSVNGSIAAKRAGMKCVI 183 Query: 515 TKKRV 529 +V Sbjct: 184 VPNKV 188 >sp|Q9X0Y1|P1254_THEMA Phosphorylated carbo

  8. AcEST: DK952111 [AcEST

    Lifescience Database Archive (English)

    Full Text Available P1254_THEMA Phosphorylated carbohydrates phosphatase T... 33 0.91 sp|P97347|RPTN_MOUSE Repetin OS=Mus muscul...----RPGVEAYLNAAKDLGLKIGL 106 Query: 582 CSTSNELAVS 611 S+S+ VS Sbjct: 107 ASSSDYKWVS 116 >sp|Q9X0Y1|P1254_THEMA Phosphorylated carboh...ydrates phosphatase TM_1254 OS=Thermotoga maritima GN=TM_1254 PE=1 SV=1 Length = 21

  9. Modeling Regular Replacement for String Constraint Solving

    Science.gov (United States)

    Fu, Xiang; Li, Chung-Chih

    2010-01-01

    Bugs in user input sanitation of software systems often lead to vulnerabilities. Among them many are caused by improper use of regular replacement. This paper presents a precise modeling of various semantics of regular substitution, such as the declarative, finite, greedy, and reluctant, using finite state transducers (FST). By projecting an FST to its input/output tapes, we are able to solve atomic string constraints, which can be applied to both the forward and backward image computation in model checking and symbolic execution of text processing programs. We report several interesting discoveries, e.g., certain fragments of the general problem can be handled using less expressive deterministic FST. A compact representation of FST is implemented in SUSHI, a string constraint solver. It is applied to detecting vulnerabilities in web applications

  10. Data completion problems solved as Nash games

    International Nuclear Information System (INIS)

    Habbal, A; Kallel, M

    2012-01-01

    The Cauchy problem for an elliptic operator is formulated as a two-player Nash game. Player (1) is given the known Dirichlet data, and uses as strategy variable the Neumann condition prescribed over the inaccessible part of the boundary. Player (2) is given the known Neumann data, and plays with the Dirichlet condition prescribed over the inaccessible boundary. The two players solve in parallel the associated Boundary Value Problems. Their respective objectives involve the gap between the non used Neumann/Dirichlet known data and the traces of the BVP's solutions over the accessible boundary, and are coupled through a difference term. We prove the existence of a unique Nash equilibrium, which turns out to be the reconstructed data when the Cauchy problem has a solution. We also prove that the completion algorithm is stable with respect to noise, and present two 3D experiments which illustrate the efficiency and stability of our algorithm.

  11. How Do College Students Solve Logarithm Questions?

    Directory of Open Access Journals (Sweden)

    Tian Abdul Aziz

    2017-02-01

    Full Text Available The purpose of the study was to investigate college students’ work with logarithm questions. Qualitative descriptive research is chosen to reach the research goal. The participants of the study were fourteen Indonesian students who were enrolled at different universities in Ankara, Turkey. They worked to solve ten logarithm questions which were classified according to the contents. After analysing their written responses, interviews were conducted to obtain further explanation about their strategies and common mistakes. The study found that participants’ works in dealing with logarithm questions comprised of (a processing base, (b holding the rule, (c separating, (d jumping, and (e conditioning. Therewith, several participants made common mistake because of misconception about logarithm, arithmetical problems, and misuse of algebra concept. Implication of the finding of the study for teaching and learning logarithm were presented.

  12. Modeling and Solving the Train Pathing Problem

    Directory of Open Access Journals (Sweden)

    Chuen-Yih Chen

    2009-04-01

    Full Text Available In a railroad system, train pathing is concerned with the assignment of trains to links and tracks, and train timetabling allocates time slots to trains. In this paper, we present an optimization heuristic to solve the train pathing and timetabling problem. This heuristic allows the dwell time of trains in a station or link to be dependent on the assigned tracks. It also allows the minimum clearance time between the trains to depend on their relative status. The heuristic generates a number of alternative paths for each train service in the initialization phase. Then it uses a neighborhood search approach to find good feasible combinations of these paths. A linear program is developed to evaluate the quality of each combination that is encountered. Numerical examples are provided.

  13. Solving moment hierarchies for chemical reaction networks

    Science.gov (United States)

    Krishnamurthy, Supriya; Smith, Eric

    2017-10-01

    The study of chemical reaction networks (CRN’s) is a very active field. Earlier well-known results (Feinberg 1987 Chem. Enc. Sci. 42 2229, Anderson et al 2010 Bull. Math. Biol. 72 1947) identify a topological quantity called deficiency, for any CRN, which, when exactly equal to zero, leads to a unique factorized steady-state for these networks. No results exist however for the steady states of non-zero-deficiency networks. In this paper, we show how to write the full moment-hierarchy for any non-zero-deficiency CRN obeying mass-action kinetics, in terms of equations for the factorial moments. Using these, we can recursively predict values for lower moments from higher moments, reversing the procedure usually used to solve moment hierarchies. We show, for non-trivial examples, that in this manner we can predict any moment of interest, for CRN’s with non-zero deficiency and non-factorizable steady states.

  14. Teaching model of problem solving Programming Fundamentals

    Directory of Open Access Journals (Sweden)

    Iván Darwin Tutillo-Arcentales

    2016-10-01

    Full Text Available The formation process has been studied by several authors in those last years, although not always focusing in the technology careers which requires of a pedagogical and didactic point of view, which promotes behaviouring changes in the teachers with impact in the quality of graduates. The purposes of this paper is: to value the pedagogical fundamentals of the formation in the career Analysis of systems,in order to promote qualitative and quantitative improvements  in the students learning. The questioner applied to students and teachers proved the difficulties in the contents related to the algorithmic procedures, which constitutes a necessary content in their formation and to them contributes other syllabus of the first level. So it is necessary to model a theoretical construction which express the new relationships established from the psychological and didactic point of view in order to solving those situations from the programing.

  15. Solving Kepler's equation using implicit functions

    Science.gov (United States)

    Mortari, Daniele; Elipe, Antonio

    2014-01-01

    A new approach to solve Kepler's equation based on the use of implicit functions is proposed here. First, new upper and lower bounds are derived for two ranges of mean anomaly. These upper and lower bounds initialize a two-step procedure involving the solution of two implicit functions. These two implicit functions, which are non-rational (polynomial) Bézier functions, can be linear or quadratic, depending on the derivatives of the initial bound values. These are new initial bounds that have been compared and proven more accurate than Serafin's bounds. The procedure reaches machine error accuracy with no more that one quadratic and one linear iterations, experienced in the "tough range", where the eccentricity is close to one and the mean anomaly to zero. The proposed method is particularly suitable for space-based applications with limited computational capability.

  16. Solving a Deconvolution Problem in Photon Spectrometry

    CERN Document Server

    Aleksandrov, D; Hille, P T; Polichtchouk, B; Kharlov, Y; Sukhorukov, M; Wang, D; Shabratova, G; Demanov, V; Wang, Y; Tveter, T; Faltys, M; Mao, Y; Larsen, D T; Zaporozhets, S; Sibiryak, I; Lovhoiden, G; Potcheptsov, T; Kucheryaev, Y; Basmanov, V; Mares, J; Yanovsky, V; Qvigstad, H; Zenin, A; Nikolaev, S; Siemiarczuk, T; Yuan, X; Cai, X; Redlich, K; Pavlinov, A; Roehrich, D; Manko, V; Deloff, A; Ma, K; Maruyama, Y; Dobrowolski, T; Shigaki, K; Nikulin, S; Wan, R; Mizoguchi, K; Petrov, V; Mueller, H; Ippolitov, M; Liu, L; Sadovsky, S; Stolpovsky, P; Kurashvili, P; Nomokonov, P; Xu, C; Torii, H; Il'kaev, R; Zhang, X; Peresunko, D; Soloviev, A; Vodopyanov, A; Sugitate, T; Ullaland, K; Huang, M; Zhou, D; Nystrand, J; Punin, V; Yin, Z; Batyunya, B; Karadzhev, K; Nazarov, G; Fil'chagin, S; Nazarenko, S; Buskenes, J I; Horaguchi, T; Djuvsland, O; Chuman, F; Senko, V; Alme, J; Wilk, G; Fehlker, D; Vinogradov, Y; Budilov, V; Iwasaki, T; Ilkiv, I; Budnikov, D; Vinogradov, A; Kazantsev, A; Bogolyubsky, M; Lindal, S; Polak, K; Skaali, B; Mamonov, A; Kuryakin, A; Wikne, J; Skjerdal, K

    2010-01-01

    We solve numerically a deconvolution problem to extract the undisturbed spectrum from the measured distribution contaminated by the finite resolution of the measuring device. A problem of this kind emerges when one wants to infer the momentum distribution of the neutral pions by detecting the it decay photons using the photon spectrometer of the ALICE LHC experiment at CERN {[}1]. The underlying integral equation connecting the sought for pion spectrum and the measured gamma spectrum has been discretized and subsequently reduced to a system of linear algebraic equations. The latter system, however, is known to be ill-posed and must be regularized to obtain a stable solution. This task has been accomplished here by means of the Tikhonov regularization scheme combined with the L-curve method. The resulting pion spectrum is in an excellent quantitative agreement with the pion spectrum obtained from a Monte Carlo simulation. (C) 2010 Elsevier B.V. All rights reserved.

  17. Solving fault diagnosis problems linear synthesis techniques

    CERN Document Server

    Varga, Andreas

    2017-01-01

    This book addresses fault detection and isolation topics from a computational perspective. Unlike most existing literature, it bridges the gap between the existing well-developed theoretical results and the realm of reliable computational synthesis procedures. The model-based approach to fault detection and diagnosis has been the subject of ongoing research for the past few decades. While the theoretical aspects of fault diagnosis on the basis of linear models are well understood, most of the computational methods proposed for the synthesis of fault detection and isolation filters are not satisfactory from a numerical standpoint. Several features make this book unique in the fault detection literature: Solution of standard synthesis problems in the most general setting, for both continuous- and discrete-time systems, regardless of whether they are proper or not; consequently, the proposed synthesis procedures can solve a specific problem whenever a solution exists Emphasis on the best numerical algorithms to ...

  18. MODELING AND SOLVING SELF-REFERENTIAL PUZZLES

    Directory of Open Access Journals (Sweden)

    Maja Bubalo

    2012-07-01

    Full Text Available The so-called self-referential puzzles are a very interesting kind of logic puzzles,aiming at developing the skill of logical thinking. A self-referential puzzle consists of asequence of questions about the puzzle itself. In this paper, we shall show some selfreferentialpuzzles, demonstrate how to model and solve them as propositional logicproblems, and how to mechanically generate new puzzles. For this, we shall make use ofthe specific advantages of Mozart/Oz system – the finite domain constraint programminglanguage and environment.We shall also show some new puzzles, according to our best knowledge not yet publishedelsewhere. The program in Mozart/Oz using our method generated these puzzles.

  19. Solving moment hierarchies for chemical reaction networks.

    Science.gov (United States)

    Krishnamurthy, Supriya; Smith, Eric

    2017-10-20

    The study of chemical reaction networks (CRN's) is a very active field. Earlier well-known results (Feinberg 1987 Chem. Enc. Sci . 42 2229, Anderson et al 2010 Bull. Math. Biol . 72 1947) identify a topological quantity called deficiency, for any CRN, which, when exactly equal to zero, leads to a unique factorized steady-state for these networks. No results exist however for the steady states of non-zero-deficiency networks. In this paper, we show how to write the full moment-hierarchy for any non-zero-deficiency CRN obeying mass-action kinetics, in terms of equations for the factorial moments. Using these, we can recursively predict values for lower moments from higher moments, reversing the procedure usually used to solve moment hierarchies. We show, for nontrivial examples, that in this manner we can predict any moment of interest, for CRN's with non-zero deficiency and non-factorizable steady states.

  20. Sleep promotes analogical transfer in problem solving.

    Science.gov (United States)

    Monaghan, Padraic; Sio, Ut Na; Lau, Sum Wai; Woo, Hoi Kei; Linkenauger, Sally A; Ormerod, Thomas C

    2015-10-01

    Analogical problem solving requires using a known solution from one problem to apply to a related problem. Sleep is known to have profound effects on memory and information restructuring, and so we tested whether sleep promoted such analogical transfer, determining whether improvement was due to subjective memory for problems, subjective recognition of similarity across related problems, or by abstract generalisation of structure. In Experiment 1, participants were exposed to a set of source problems. Then, after a 12-h period involving sleep or wake, they attempted target problems structurally related to the source problems but with different surface features. Experiment 2 controlled for time of day effects by testing participants either in the morning or the evening. Sleep improved analogical transfer, but effects were not due to improvements in subjective memory or similarity recognition, but rather effects of structural generalisation across problems. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Solving stochastic inflation for arbitrary potentials

    International Nuclear Information System (INIS)

    Martin, Jerome; Musso, Marcello

    2006-01-01

    A perturbative method for solving the Langevin equation of inflationary cosmology in the presence of backreaction is presented. In the Gaussian approximation, the method permits an explicit calculation of the probability distribution of the inflaton field for an arbitrary potential, with or without the volume effects taken into account. The perturbative method is then applied to various concrete models, namely, large field, small field, hybrid, and running mass inflation. New results on the stochastic behavior of the inflaton field in those models are obtained. In particular, it is confirmed that the stochastic effects can be important in new inflation while it is demonstrated they are negligible in (vacuum dominated) hybrid inflation. The case of stochastic running mass inflation is discussed in some details and it is argued that quantum effects blur the distinction between the four classical versions of this model. It is also shown that the self-reproducing regime is likely to be important in this case

  2. "I'm Not Very Good at Solving Problems": An Exploration of Students' Problem Solving Behaviours

    Science.gov (United States)

    Muir, Tracey; Beswick, Kim; Williamson, John

    2008-01-01

    This paper reports one aspect of a larger study which looked at the strategies used by a selection of grade 6 students to solve six non-routine mathematical problems. The data revealed that the students exhibited many of the behaviours identified in the literature as being associated with novice and expert problem solvers. However, the categories…

  3. How to make university students solve physics problems requiring mathematical skills: The "Adventurous Problem Solving" approach

    NARCIS (Netherlands)

    de Mul, F.F.M.; Martin Batlle, C.; Martin i Batlle, Cristina; de Bruijn, Imme; Rinzema, K.; Rinzema, Kees

    2003-01-01

    Teaching physics to first-year university students (in the USA: junior/senior level) is often hampered by their lack of skills in the underlying mathematics, and that in turn may block their understanding of the physics and their ability to solve problems. Examples are vector algebra, differential

  4. Solving Math Problems Approximately: A Developmental Perspective.

    Directory of Open Access Journals (Sweden)

    Dana Ganor-Stern

    Full Text Available Although solving arithmetic problems approximately is an important skill in everyday life, little is known about the development of this skill. Past research has shown that when children are asked to solve multi-digit multiplication problems approximately, they provide estimates that are often very far from the exact answer. This is unfortunate as computation estimation is needed in many circumstances in daily life. The present study examined 4th graders, 6th graders and adults' ability to estimate the results of arithmetic problems relative to a reference number. A developmental pattern was observed in accuracy, speed and strategy use. With age there was a general increase in speed, and an increase in accuracy mainly for trials in which the reference number was close to the exact answer. The children tended to use the sense of magnitude strategy, which does not involve any calculation but relies mainly on an intuitive coarse sense of magnitude, while the adults used the approximated calculation strategy which involves rounding and multiplication procedures, and relies to a greater extent on calculation skills and working memory resources. Importantly, the children were less accurate than the adults, but were well above chance level. In all age groups performance was enhanced when the reference number was smaller (vs. larger than the exact answer and when it was far (vs. close from it, suggesting the involvement of an approximate number system. The results suggest the existence of an intuitive sense of magnitude for the results of arithmetic problems that might help children and even adults with difficulties in math. The present findings are discussed in the context of past research reporting poor estimation skills among children, and the conditions that might allow using children estimation skills in an effective manner.

  5. Solving Math Problems Approximately: A Developmental Perspective.

    Science.gov (United States)

    Ganor-Stern, Dana

    2016-01-01

    Although solving arithmetic problems approximately is an important skill in everyday life, little is known about the development of this skill. Past research has shown that when children are asked to solve multi-digit multiplication problems approximately, they provide estimates that are often very far from the exact answer. This is unfortunate as computation estimation is needed in many circumstances in daily life. The present study examined 4th graders, 6th graders and adults' ability to estimate the results of arithmetic problems relative to a reference number. A developmental pattern was observed in accuracy, speed and strategy use. With age there was a general increase in speed, and an increase in accuracy mainly for trials in which the reference number was close to the exact answer. The children tended to use the sense of magnitude strategy, which does not involve any calculation but relies mainly on an intuitive coarse sense of magnitude, while the adults used the approximated calculation strategy which involves rounding and multiplication procedures, and relies to a greater extent on calculation skills and working memory resources. Importantly, the children were less accurate than the adults, but were well above chance level. In all age groups performance was enhanced when the reference number was smaller (vs. larger) than the exact answer and when it was far (vs. close) from it, suggesting the involvement of an approximate number system. The results suggest the existence of an intuitive sense of magnitude for the results of arithmetic problems that might help children and even adults with difficulties in math. The present findings are discussed in the context of past research reporting poor estimation skills among children, and the conditions that might allow using children estimation skills in an effective manner.

  6. The Effects of GO Solve Word Problems Math Intervention on Applied Problem Solving Skills of Low Performing Fifth Grade Students

    Science.gov (United States)

    Fede, Jessica L.

    2010-01-01

    This research investigation examined the effects of "GO Solve Word Problems" math intervention on problem-solving skills of struggling 5th grade students. In a randomized controlled study, 16 5th grade students were given a 12-week intervention of "GO Solve", a computer-based program designed to teach schema-based instruction…

  7. Modos de vida maritima en Europa

    DEFF Research Database (Denmark)

    Højrup, Thomas; Schriewer, Klaus

    2013-01-01

    Analyse og syntese af de biologiske livsformers betydning som mulighedsbetingelse for de anvendte teknologier og fangstmåders betydning som mulighedsbetingelse for de to produktionsmåders sameksistens i euroæisk fiskeri i 500 år og deres betydning som mulighedsbetingelser for de sameksisterende k...

  8. Improving mathematical problem solving skills through visual media

    Science.gov (United States)

    Widodo, S. A.; Darhim; Ikhwanudin, T.

    2018-01-01

    The purpose of this article was to find out the enhancement of students’ mathematical problem solving by using visual learning media. The ability to solve mathematical problems is the ability possessed by students to solve problems encountered, one of the problem-solving model of Polya. This preliminary study was not to make a model, but it only took a conceptual approach by comparing the various literature of problem-solving skills by linking visual learning media. The results of the study indicated that the use of learning media had not been appropriated so that the ability to solve mathematical problems was not optimal. The inappropriateness of media use was due to the instructional media that was not adapted to the characteristics of the learners. Suggestions that can be given is the need to develop visual media to increase the ability to solve problems.

  9. Flexibility in Mathematics Problem Solving Based on Adversity Quotient

    Science.gov (United States)

    Dina, N. A.; Amin, S. M.; Masriyah

    2018-01-01

    Flexibility is an ability which is needed in problem solving. One of the ways in problem solving is influenced by Adversity Quotient (AQ). AQ is the power of facing difficulties. There are three categories of AQ namely climber, camper, and quitter. This research is a descriptive research using qualitative approach. The aim of this research is to describe flexibility in mathematics problem solving based on Adversity Quotient. The subjects of this research are climber student, camper student, and quitter student. This research was started by giving Adversity Response Profile (ARP) questioner continued by giving problem solving task and interviews. The validity of data measurement was using time triangulation. The results of this research shows that climber student uses two strategies in solving problem and doesn’t have difficulty. The camper student uses two strategies in solving problem but has difficulty to finish the second strategies. The quitter student uses one strategy in solving problem and has difficulty to finish it.

  10. Generating and Solving Symbolic Parity Games

    Directory of Open Access Journals (Sweden)

    Gijs Kant

    2014-07-01

    Full Text Available We present a new tool for verification of modal mu-calculus formulae for process specifications, based on symbolic parity games. It enhances an existing method, that first encodes the problem to a Parameterised Boolean Equation System (PBES and then instantiates the PBES to a parity game. We improved the translation from specification to PBES to preserve the structure of the specification in the PBES, we extended LTSmin to instantiate PBESs to symbolic parity games, and implemented the recursive parity game solving algorithm by Zielonka for symbolic parity games. We use Multi-valued Decision Diagrams (MDDs to represent sets and relations, thus enabling the tools to deal with very large systems. The transition relation is partitioned based on the structure of the specification, which allows for efficient manipulation of the MDDs. We performed two case studies on modular specifications, that demonstrate that the new method has better time and memory performance than existing PBES based tools and can be faster (but slightly less memory efficient than the symbolic model checker NuSMV.

  11. Solving graph problems with dynamic computation structures

    Science.gov (United States)

    Babb, Jonathan W.; Frank, Matthew; Agarwal, Anant

    1996-10-01

    We introduce dynamic computation structures (DCS), a compilation technique to produce dynamic code for reconfigurable computing. DCS specializes directed graph instances into user-level hardware for reconfigurable architectures. Several problems such as shortest path and transitive closure exhibit the general properties of closed semirings, an algebraic structure for solving directed paths. Motivating our application domain choice of closed semiring problems is the fact that logic emulation software already maps a special case of directed graphs, namely logic netlists, onto arrays of field programmable gate arrays (FPGA). A certain type of logic emulation software called virtual wires further allows an FPGA array to be viewed as a machine-independent computing fabric. Thus, a virtual wires compiler, coupled with front-end commercial behavioral logic synthesis software, enables automatic behavioral compilation into a multi-FPGA computing fabric. We have implemented a DCS front-end compiler to parallelize the entire inner loop of the classic Bellman-Ford algorithm into synthesizable behavioral verilog. Leveraging virtual wire compilation and behavioral synthesis, we have automatically generated designs of 14 to 261 FPGAs from a single graph instance. We achieve speedups proportional to the number of graph edges - - from 10X to almost 400X versus a 125 SPECint SparcStation 10.

  12. Projective geometry solved problems and theory review

    CERN Document Server

    Fortuna, Elisabetta; Pardini, Rita

    2016-01-01

    This book starts with a concise but rigorous overview of the basic notions of projective geometry, using straightforward and modern language. The goal is not only to establish the notation and terminology used, but also to offer the reader a quick survey of the subject matter. In the second part, the book presents more than 200 solved problems, for many of which several alternative solutions are provided. The level of difficulty of the exercises varies considerably: they range from computations to harder problems of a more theoretical nature, up to some actual complements of the theory. The structure of the text allows the reader to use the solutions of the exercises both to master the basic notions and techniques and to further their knowledge of the subject, thus learning some classical results not covered in the first part of the book. The book addresses the needs of undergraduate and graduate students in the theoretical and applied sciences, and will especially benefit those readers with a solid grasp of ...

  13. Solved? The reductive radiation chemistry of alanine.

    Science.gov (United States)

    Pauwels, Ewald; De Cooman, Hendrik; Waroquier, Michel; Hole, Eli O; Sagstuen, Einar

    2014-02-14

    The structural changes throughout the entire reductive radiation-induced pathway of l-α-alanine are solved on an atomistic level with the aid of periodic DFT and nudged elastic band (NEB) simulations. This yields unprecedented information on the conformational changes taking place, including the protonation state of the carboxyl group in the "unstable" and "stable" alanine radicals and the internal transformation converting these two radical variants at temperatures above 220 K. The structures of all stable radicals were verified by calculating EPR properties and comparing those with experimental data. The variation of the energy throughout the full radiochemical process provides crucial insight into the reason why these structural changes and rearrangements occur. Starting from electron capture, the excess electron quickly localizes on the carbon of a carboxyl group, which pyramidalizes and receives a proton from the amino group of a neighboring alanine molecule, forming a first stable radical species (up to 150 K). In the temperature interval 150-220 K, this radical deaminates and deprotonates at the carboxyl group, the detached amino group undergoes inversion and its methyl group sustains an internal rotation. This yields the so-called "unstable alanine radical". Above 220 K, triggered by the attachment of an additional proton on the detached amino group, the radical then undergoes an internal rotation in the reverse direction, giving rise to the "stable alanine radical", which is the final stage in the reductive radiation-induced decay of alanine.

  14. Solved Problems in Quantum and Statistical Mechanics

    CERN Document Server

    Cini, Michele; Sbragaglia, Mauro

    2012-01-01

    This work arises from our teaching this subject during many years. The vast majority of these exercises are the exams we gave to our students in this period. We carefully selected the subjects of the exercises to cover all the material which is most needed  and which is treated in the most well known texts on these subjects. Each exercise is carefully solved in full details, explaining the theory behind the solution with particular care for those issues that, from our experience, are found most difficult from the average student. Indeed, several exercises are designed to throw light on  aspects of the theory that, for one reason or another, are usually neglected with the result to make the students feel uneasy about them. In fact most students get acquainted just with the more common manipulations,  which are illustrated by  many examples in textbooks. Our exercises never require extensive calculations  but tend to be somewhat unusual  and force the solver  to think about the problem starting from the ...

  15. A new technique for solving puzzles.

    Science.gov (United States)

    Makridis, Michael; Papamarkos, Nikos

    2010-06-01

    This paper proposes a new technique for solving jigsaw puzzles. The novelty of the proposed technique is that it provides an automatic jigsaw puzzle solution without any initial restriction about the shape of pieces, the number of neighbor pieces, etc. The proposed technique uses both curve- and color-matching similarity features. A recurrent procedure is applied, which compares and merges puzzle pieces in pairs, until the original puzzle image is reformed. Geometrical and color features are extracted on the characteristic points (CPs) of the puzzle pieces. CPs, which can be considered as high curvature points, are detected by a rotationally invariant corner detection algorithm. The features which are associated with color are provided by applying a color reduction technique using the Kohonen self-organized feature map. Finally, a postprocessing stage checks and corrects the relative position between puzzle pieces to improve the quality of the resulting image. Experimental results prove the efficiency of the proposed technique, which can be further extended to deal with even more complex jigsaw puzzle problems.

  16. Problem-solving in a Constructivist Environment

    Directory of Open Access Journals (Sweden)

    Lee Chien Sing

    1999-01-01

    Full Text Available The dynamic challenges of an increasingly borderless world buoyed by advances in telecommunications and information technology has resulted in educational reform and subsequently, a reconceptualisation of what constitutes a learner, learning and the influence of the learning environment on the process of learning. In keeping up with the changing trends and challenges of an increasingly networked, dynamic and challenging international community, means to provide an alternative environment that stimulates inquiry and equips learners with the skills needed to manage technological change and innovations must be considered. This paper discusses the importance of interaction, cognition and context, collaboration in a networked computer-mediated environment, the problem-solving approach as a catalyst in stimulating creative and critical thinking and in providing context for meaningful interaction and whether the interactive environment created through computer-mediated collaboration will motivate learners to be responsible for their own learning and be independent thinkers. The sample involved learners from three schools in three different countries. Findings conclude that a rich interactive environment must be personally relevant to the learner by simulating authentic problems without lowering the degree of cognitive complexity. Review in curriculum, assessment and teacher training around constructivist principles are also imperative as these interrelated factors form part of the learning process system.

  17. Solve the Dilemma of Over-Simplification

    Science.gov (United States)

    Schmitt, Gerhard

    Complexity science can help to understand the functioning and the interaction of the components of a city. In 1965, Christopher Alexander gave in his book A city is not a tree a description of the complex nature of urban organization. At this time, neither high-speed computers nor urban big data existed. Today, Luis Bettencourt et al. use complexity science to analyze data for countries, regions, or cities. The results can be used globally in other cities. Objectives of complexity science with regard to future cities are the observation and identification of tendencies and regularities in behavioral patterns, and to find correlations between them and spatial configurations. Complex urban systems cannot be understood in total yet. But research focuses on describing the system by finding some simple, preferably general and emerging patterns and rules that can be used for urban planning. It is important that the influencing factors are not just geo-spatial patterns but also consider variables which are important for the design quality. Complexity science is a way to solve the dilemma of oversimplification of insights from existing cities and their applications to new cities. An example: The effects of streets, public places and city structures on citizens and their behavior depend on how they are perceived. To describe this perception, it is not sufficient to consider only particular characteristics of the urban environment. Different aspects play a role and influence each other. Complexity science could take this fact into consideration and handle the non-linearity of the system...

  18. Solving the Examination Timetabling Problem in GPUs

    Directory of Open Access Journals (Sweden)

    Vasileios Kolonias

    2014-07-01

    Full Text Available The examination timetabling problem belongs to the class of combinatorial optimization problems and is of great importance for every University. In this paper, a hybrid evolutionary algorithm running on a GPU is employed to solve the examination timetabling problem. The hybrid evolutionary algorithm proposed has a genetic algorithm component and a greedy steepest descent component. The GPU computational capabilities allow the use of very large population sizes, leading to a more thorough exploration of the problem solution space. The GPU implementation, depending on the size of the problem, is up to twenty six times faster than the identical single-threaded CPU implementation of the algorithm. The algorithm is evaluated with the well known Toronto datasets and compares well with the best results found in the bibliography. Moreover, the selection of the encoding of the chromosomes and the tournament selection size as the population grows are examined and optimized. The compressed sparse row format is used for the conflict matrix and was proven essential to the process, since most of the datasets have a small conflict density, which translates into an extremely sparse matrix.

  19. Can Architecture Design Solve Social Problem?

    Science.gov (United States)

    Ginting, S. W.; TSB Darjosanjoto, E.; Sulistyarso, H.

    2017-03-01

    Most of architects and urban designers believe physical design gives impact on our social life. For example, a sign or landmark in the middle of a city makes people find orientation easier. In vice verse, most of social scientists believe it is social dynamic that plays role in shaping our space. How people spend their time moving from real space into cyber space is a proof that life style and IT give impact to space usage. This paper argues that interaction between physical design and social change is a two ways process. Both design aspect and social dynamic influence each other. This paper aims to examine how designing of gated community plays important role in increasing or decreasing segregation, both spatially and socially. The paper explores some architectural design principles applied in a gated community called CitraLand in west Surabaya, Indonesia, and addresses segregation between CitraLanders and outside kampung. We find CitraLand is designed openly and fully accessible for outsiders. It provides public spaces and several accessible gates and streets without walls and fences making all places inside and outside CitraLand spatially integrated. What’s interesting is it still reinforces social segregation due to its policy on prohibiting using the public park. We believe CitraLand’s planning and designing has successfully solved segregation problem spatially not socially.

  20. Rejection Sensitivity and Depression: Indirect Effects Through Problem Solving.

    Science.gov (United States)

    Kraines, Morganne A; Wells, Tony T

    2017-01-01

    Rejection sensitivity (RS) and deficits in social problem solving are risk factors for depression. Despite their relationship to depression and the potential connection between them, no studies have examined RS and social problem solving together in the context of depression. As such, we examined RS, five facets of social problem solving, and symptoms of depression in a young adult sample. A total of 180 participants completed measures of RS, social problem solving, and depressive symptoms. We used bootstrapping to examine the indirect effect of RS on depressive symptoms through problem solving. RS was positively associated with depressive symptoms. A negative problem orientation, impulsive/careless style, and avoidance style of social problem solving were positively associated with depressive symptoms, and a positive problem orientation was negatively associated with depressive symptoms. RS demonstrated an indirect effect on depressive symptoms through two social problem-solving facets: the tendency to view problems as threats to one's well-being and an avoidance problem-solving style characterized by procrastination, passivity, or overdependence on others. These results are consistent with prior research that found a positive association between RS and depression symptoms, but this is the first study to implicate specific problem-solving deficits in the relationship between RS and depression. Our results suggest that depressive symptoms in high RS individuals may result from viewing problems as threats and taking an avoidant, rather than proactive, approach to dealing with problems. These findings may have implications for problem-solving interventions for rejection sensitive individuals.

  1. Forward problem of electrocardiography: is it solved?

    Science.gov (United States)

    Bear, Laura R; Cheng, Leo K; LeGrice, Ian J; Sands, Gregory B; Lever, Nigel A; Paterson, David J; Smaill, Bruce H

    2015-06-01

    The relationship between epicardial and body surface potentials defines the forward problem of electrocardiography. A robust formulation of the forward problem is instrumental to solving the inverse problem, in which epicardial potentials are computed from known body surface potentials. Here, the accuracy of different forward models has been evaluated experimentally. Body surface and epicardial potentials were recorded simultaneously in anesthetized closed-chest pigs (n=5) during sinus rhythm, and epicardial and endocardial ventricular pacing (65 records in total). Body surface potentials were simulated from epicardial recordings using experiment-specific volume conductor models constructed from magnetic resonance imaging. Results for homogeneous (isotropic electric properties) and inhomogeneous (incorporating lungs, anisotropic skeletal muscle, and subcutaneous fat) forward models were compared with measured body surface potentials. Correlation coefficients were 0.85±0.08 across all animals and activation sequences with no significant difference between homogeneous and inhomogeneous solutions (P=0.85). Despite this, there was considerable variance between simulated and measured body surface potential distributions. Differences between the body surface potential extrema predicted with homogeneous forward models were 55% to 78% greater than observed (P<0.05) and attenuation of potentials adjacent to extrema were 10% to 171% greater (P<0.03). The length and orientation of the vector between potential extrema were also significantly different. Inclusion of inhomogeneous electric properties in the forward model reduced, but did not eliminate these differences. These results demonstrate that homogeneous volume conductor models introduce substantial spatial inaccuracies in forward problem solutions. This probably affects the precision of inverse reconstructions of cardiac potentials, in which this assumption is made. © 2015 American Heart Association, Inc.

  2. The semantic system is involved in mathematical problem solving.

    Science.gov (United States)

    Zhou, Xinlin; Li, Mengyi; Li, Leinian; Zhang, Yiyun; Cui, Jiaxin; Liu, Jie; Chen, Chuansheng

    2018-02-01

    Numerous studies have shown that the brain regions around bilateral intraparietal cortex are critical for number processing and arithmetical computation. However, the neural circuits for more advanced mathematics such as mathematical problem solving (with little routine arithmetical computation) remain unclear. Using functional magnetic resonance imaging (fMRI), this study (N = 24 undergraduate students) compared neural bases of mathematical problem solving (i.e., number series completion, mathematical word problem solving, and geometric problem solving) and arithmetical computation. Direct subject- and item-wise comparisons revealed that mathematical problem solving typically had greater activation than arithmetical computation in all 7 regions of the semantic system (which was based on a meta-analysis of 120 functional neuroimaging studies on semantic processing). Arithmetical computation typically had greater activation in the supplementary motor area and left precentral gyrus. The results suggest that the semantic system in the brain supports mathematical problem solving. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. The effects of monitoring environment on problem-solving performance.

    Science.gov (United States)

    Laird, Brian K; Bailey, Charles D; Hester, Kim

    2018-01-01

    While effective and efficient solving of everyday problems is important in business domains, little is known about the effects of workplace monitoring on problem-solving performance. In a laboratory experiment, we explored the monitoring environment's effects on an individual's propensity to (1) establish pattern solutions to problems, (2) recognize when pattern solutions are no longer efficient, and (3) solve complex problems. Under three work monitoring regimes-no monitoring, human monitoring, and electronic monitoring-114 participants solved puzzles for monetary rewards. Based on research related to worker autonomy and theory of social facilitation, we hypothesized that monitored (versus non-monitored) participants would (1) have more difficulty finding a pattern solution, (2) more often fail to recognize when the pattern solution is no longer efficient, and (3) solve fewer complex problems. Our results support the first two hypotheses, but in complex problem solving, an interaction was found between self-assessed ability and the monitoring environment.

  4. Social problem-solving in Chinese baccalaureate nursing students.

    Science.gov (United States)

    Fang, Jinbo; Luo, Ying; Li, Yanhua; Huang, Wenxia

    2016-11-01

    To describe social problem solving in Chinese baccalaureate nursing students. A descriptive cross-sectional study was conducted with a cluster sample of 681 Chinese baccalaureate nursing students. The Chinese version of the Social Problem-Solving scale was used. Descriptive analyses, independent t-test and one-way analysis of variance were applied to analyze the data. The final year nursing students presented the highest scores of positive social problem-solving skills. Students with experiences of self-directed and problem-based learning presented significantly higher scores in Positive Problem Orientation subscale. The group with Critical thinking training experience, however, displayed higher negative problem solving scores compared with nonexperience group. Social problem solving abilities varied based upon teaching-learning strategies. Self-directed and problem-based learning may be recommended as effective way to improve social problem-solving ability. © 2016 Chinese Cochrane Center, West China Hospital of Sichuan University and John Wiley & Sons Australia, Ltd.

  5. Using Analogy to Solve a Three-Step Physics Problem

    Science.gov (United States)

    Lin, Shih-Yin; Singh, Chandralekha

    2010-10-01

    In a companion paper, we discuss students' ability to take advantage of what they learn from a solved problem and transfer their learning to solve a quiz problem that has different surface features but the same underlying physics principles. Here, we discuss students' ability to perform analogical reasoning between another pair of problems. Both the problems can be solved using the same physics principles. However, the solved problem provided was a two-step problem (which can be solved by decomposing it into two sub-problems) while the quiz problem was a three-step problem. We find that it is challenging for students to extend what they learned from a two-step problem to solve a three-step problem.

  6. Affect and mathematical problem solving a new perspective

    CERN Document Server

    Adams, Verna

    1989-01-01

    Research on cognitive aspects of mathematical problem solving has made great progress in recent years, but the relationship of affective factors to problem-solving performance has been a neglected research area. The purpose of Affect and Mathematical Problem Solving: A New Perspective is to show how the theories and methods of cognitive science can be extended to include the role of affect in mathematical problem solving. The book presents Mandler's theory of emotion and explores its implications for the learning and teaching of mathematical problem solving. Also, leading researchers from mathematics, education, and psychology report how they have integrated affect into their own cognitive research. The studies focus on metacognitive processes, aesthetic influences on expert problem solvers, teacher decision-making, technology and teaching problem solving, and beliefs about mathematics. The results suggest how emotional factors like anxiety, frustration, joy, and satisfaction can help or hinder performance in...

  7. Self-affirmation improves problem-solving under stress.

    Science.gov (United States)

    Creswell, J David; Dutcher, Janine M; Klein, William M P; Harris, Peter R; Levine, John M

    2013-01-01

    High levels of acute and chronic stress are known to impair problem-solving and creativity on a broad range of tasks. Despite this evidence, we know little about protective factors for mitigating the deleterious effects of stress on problem-solving. Building on previous research showing that self-affirmation can buffer stress, we tested whether an experimental manipulation of self-affirmation improves problem-solving performance in chronically stressed participants. Eighty undergraduates indicated their perceived chronic stress over the previous month and were randomly assigned to either a self-affirmation or control condition. They then completed 30 difficult remote associate problem-solving items under time pressure in front of an evaluator. Results showed that self-affirmation improved problem-solving performance in underperforming chronically stressed individuals. This research suggests a novel means for boosting problem-solving under stress and may have important implications for understanding how self-affirmation boosts academic achievement in school settings.

  8. Identifying Students for Intervention in Math Problem Solving: An Evaluation of Fluency-Based Word Problem Solving Measures

    OpenAIRE

    Sisco-Taylor, Dennis T.

    2014-01-01

    This study examined the extent to which initial performance, and growth on an experimental CBM word problem solving fluency measure (WPSF) were predictive of student performance on criterion measures of math problem solving. In addition, the extent to which WPSF could correctly classify students as a function of risk status was evaluated. Alternate forms of the WPSF measure were administered to 142 third grade students, along with multiple criterion measures of math problem solving. Results i...

  9. The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor.

    Energy Technology Data Exchange (ETDEWEB)

    Korolev, S.; Ikeguchi, Y.; Skarina, T.; Beasley, S.; Arrowsmith, C.; Edwards, A.; Joachimiak, A.; Pegg, A. E.; Savchenko, A.; Pennsylvania State Univ. Coll. of Medicine; Milton S. Hershey Medical Center; Banting and Best Department of Medical Research; Univ. of Health Network

    2002-01-01

    Polyamines are essential in all branches of life. Spermidine synthase (putrescine aminopropyltransferase, PAPT) catalyzes the biosynthesis of spermidine, a ubiquitous polyamine. The crystal structure of the PAPT from Thermotoga maritima (TmPAPT) has been solved to 1.5 Angstroms resolution in the presence and absence of AdoDATO (S-adenosyl-1,8-diamino-3-thiooctane), a compound containing both substrate and product moieties. This, the first structure of an aminopropyltransferase, reveals deep cavities for binding substrate and cofactor, and a loop that envelops the active site. The AdoDATO binding site is lined with residues conserved in PAPT enzymes from bacteria to humans, suggesting a universal catalytic mechanism. Other conserved residues act sterically to provide a structural basis for polyamine specificity. The enzyme is tetrameric; each monomer consists of a C-terminal domain with a Rossmann-like fold and an N-terminal {beta}-stranded domain. The tetramer is assembled using a novel barrel-type oligomerization motif.

  10. How do they solve it? An insight into the learner’s approach to the mechanism of physics problem solving

    Directory of Open Access Journals (Sweden)

    Balasubrahmanya Hegde

    2012-03-01

    Full Text Available A perceived difficulty is associated with physics problem solving from a learner’s viewpoint, arising out of a multitude of reasons. In this paper, we have examined the microstructure of students’ thought processes during physics problem solving by combining the analysis of responses to multiple-choice questions and semistructured student interviews. Design of appropriate scaffoldings serves as pointers to the identification of student problem solving difficulties. An analysis of the results suggests the necessity of identification of the skill sets required for developing better problem solving abilities.

  11. Solving the minimum flow problem with interval bounds and flows

    Indian Academy of Sciences (India)

    ... with crisp data. In this paper, the idea of Ghiyasvand was extended for solving the minimum flow problem with interval-valued lower, upper bounds and flows. This problem can be solved using two minimum flow problems with crisp data. Then, this result is extended to networks with fuzzy lower, upper bounds and flows.

  12. George Pólya & Problem Solving ... An Appreciation

    Indian Academy of Sciences (India)

    IAS Admin

    Studying the methods of solving problems, we perceive another face of ... [For] example, group theory has concentrated ideas which formerly were found ... great contributions was in bringing energy and attention to bear on the field of pedagogy. Agreatdiscovery solves a great problem but there is a grain of discovery in the.

  13. The Effects of Iliad on Medical Student Problem Solving

    OpenAIRE

    Turner, Charles W.; Williamson, John; Lincoln, Michael J.; Haug, Peter J.; Buchanan, James; Anderson, Curtis; Grant, Morgan; Cundick, Robert; Warner, Homer R.

    1990-01-01

    The present study examined the effects of the Iliad expert system on diagnostic problem solving of third-year (n = 97) medical students. Students used Iliad to work-up simulated cases to supplement the education they received in their medicine clerkship. The results of the research provided evidence that the Iliad expert system did improve student diagnostic problem solving and decision making.

  14. The Effect of Strategy on Problem Solving: An FMRI Study

    Science.gov (United States)

    Newman, Sharlene D.; Pruce, Benjamin; Rusia, Akash; Burns, Thomas, Jr.

    2010-01-01

    fMRI was used to examine the differential effect of two problem-solving strategies. Participants were trained to use both a pictorial/spatial and a symbolic/algebraic strategy to solve word problems. While these two strategies activated similar cortical regions, a number of differences were noted in the level of activation. These differences…

  15. Problem Solving Frameworks for Mathematics and Software Development

    Science.gov (United States)

    McMaster, Kirby; Sambasivam, Samuel; Blake, Ashley

    2012-01-01

    In this research, we examine how problem solving frameworks differ between Mathematics and Software Development. Our methodology is based on the assumption that the words used frequently in a book indicate the mental framework of the author. We compared word frequencies in a sample of 139 books that discuss problem solving. The books were grouped…

  16. Relationship between Problem-Solving Ability and Career Maturity ...

    African Journals Online (AJOL)

    This study investigated the relationship between problem-solving ability and career maturity of secondary school students in Ibadan, Oyo State, Nigeria. 230 final year secondary school students completed self-report measures of problem solving and career maturity. Multiple regression analysis was used to analyse the data ...

  17. Solving the uncalibrated photometric stereo problem using total variation

    DEFF Research Database (Denmark)

    Quéau, Yvain; Lauze, Francois Bernard; Durou, Jean-Denis

    2013-01-01

    In this paper we propose a new method to solve the problem of uncalibrated photometric stereo, making very weak assumptions on the properties of the scene to be reconstructed. Our goal is to solve the generalized bas-relief ambiguity (GBR) by performing a total variation regularization of both...

  18. Basic school pupils' strategies in solving subtraction problems ...

    African Journals Online (AJOL)

    This article reports some of the strategies basic schools children apply in solving subtraction problems. The purpose of the study was to see whether the semantic structure of mathematical problems influences children's choice of strategy in solving a subtraction problem. Mathematics Connection Vol. 4 2004: 31-37 ...

  19. Using problem-solving instruction to overcome high school ...

    African Journals Online (AJOL)

    Using problem-solving instruction to overcome high school chemistry students' difficulties with stoichiometric problems. ... African Journal of Educational Studies in Mathematics and Sciences ... The study sought to find out the difficulties encountered by high school chemistry students when solving stoichiometric problems.

  20. Assessment for Intervention: A Problem Solving Approach. Second Edition

    Science.gov (United States)

    Brown­ Chidsey, Rachel, Ed.; Andren, Kristina J., Ed.

    2015-01-01

    Problem-­solving assessment is an essential component of multi-­tiered systems of support such as response to intervention (RTI) and positive behavioral interventions and supports (PBIS). This authoritative work provides a complete guide to implementing a wide range of problem­-solving assessment methods, including: (1) functional behavioral…

  1. Instructional Design-Based Research on Problem Solving Strategies

    Science.gov (United States)

    Emre-Akdogan, Elçin; Argün, Ziya

    2016-01-01

    The main goal of this study is to find out the effect of the instructional design method on the enhancement of problem solving abilities of students. Teaching sessions were applied to ten students who are in 11th grade, to teach them problem solving strategies which are working backwards, finding pattern, adopting a different point of view,…

  2. Teaching Problem-Solving Skills to Nuclear Engineering Students

    Science.gov (United States)

    Waller, E.; Kaye, M. H.

    2012-01-01

    Problem solving is an essential skill for nuclear engineering graduates entering the workforce. Training in qualitative and quantitative aspects of problem solving allows students to conceptualise and execute solutions to complex problems. Solutions to problems in high consequence fields of study such as nuclear engineering require rapid and…

  3. Using Everyday Materials To Promote Problem Solving in Toddlers.

    Science.gov (United States)

    Segatti, Laura; Brown-DuPaul, Judy; Keyes, Tracy L.

    2003-01-01

    Outlines benefits of and skills involved in problem solving. Details how an environment rich in materials that foster cause-and-effect or trial-and-error explorations promote cognitive development among toddlers. Offers examples of problem-solving experiences and lists materials for use in curriculum planning. Describes the teacher' role as one of…

  4. Strategies, Not Solutions: Involving Students in Problem Solving.

    Science.gov (United States)

    Von Kuster, Lee N.

    1984-01-01

    Defines problem solving, discusses the use of problems developed by students that are relevant to their own lives, presents examples of practical mathematics problems that deal with local situations, discusses fringe benefits of this type of problem solving, and addresses teachers' concern that this method consumes too much time. (MBR)

  5. Explanation and the Theory of Expert Problem Solving

    Science.gov (United States)

    1990-02-01

    clasification strategy for diagrsls. Bennett [21 presents COAST, a shell for the design of configuration problem solving systems. All these approaches share the...category may exist in more than one 1) Not all classification problems are nece ssaiily solved classification hierarchy, e.g.. viral hepatitis is a con- as

  6. students' preference of method of solving simultaneous equations

    African Journals Online (AJOL)

    Ugboduma,Samuel.O.

    More so, they will be reposition in order to solve other mathematical problems involving applications of simultaneous equations methods of solutions. Research questions. The following research questions were asked: i what proportion of SSS2 students showed preference for one type of method of solving simultaneous.

  7. An approach for solving linear fractional programming problems ...

    African Journals Online (AJOL)

    An approach for solving linear fractional programming problems. ... Journal of the Nigerian Association of Mathematical Physics ... The paper presents a new approach for solving a fractional linear programming problem in which the objective function is a linear fractional function, while the constraint functions are in the form ...

  8. Social Problem Solving and Aggression: The Role of Depression

    Science.gov (United States)

    Ozdemir, Yalcin; Kuzucu, Yasar; Koruklu, Nermin

    2013-01-01

    The purpose of the present study was to examine direct and indirect relations among social problem-solving, depression, and aggression, as well as the mediating role of depression in the link between social problem-solving and aggression among Turkish youth. Data for the present study were collected from 413 adolescents. The participants' age…

  9. Problem solving and problem strategies in the teaching and learning ...

    African Journals Online (AJOL)

    Perennial poor performance recorded annually in both internal and external examinations in Mathematics has been a great concern for the Mathematics Educators in Nigeria. This paper discusses problem-solving and influence of problem-solving strategies on students' performance in mathematics. The concept of ...

  10. The Effects of Service Learning on Student Problem Solving

    Science.gov (United States)

    Guo, Fangfang; Yao, Meilin; Wang, Cong; Yan, Wenfan; Zong, Xiaoli

    2016-01-01

    Previous research indicated that service learning (SL) is an effective pedagogy to improve students' problem-solving ability and increase their classroom engagement. However, studies on SL are rare in China. This study examined the effects of SL on the problem solving of Chinese undergraduate students as well as the mechanism through which it…

  11. Cognitive Load in Algebra: Element Interactivity in Solving Equations

    Science.gov (United States)

    Ngu, Bing Hiong; Chung, Siu Fung; Yeung, Alexander Seeshing

    2015-01-01

    Central to equation solving is the maintenance of equivalence on both sides of the equation. However, when the process involves an interaction of multiple elements, solving an equation can impose a high cognitive load. The balance method requires operations on both sides of the equation, whereas the inverse method involves operations on one side…

  12. Fourth order compact finite difference method for solving singularly ...

    African Journals Online (AJOL)

    A numerical method based on finite difference scheme with uniform mesh is presented for solving singularly perturbed two-point boundary value problems of 1D reaction-diffusion equations. First, the derivatives of the given differential equation is replaced by the finite difference approximations and then, solved by using ...

  13. A Markov Model Analysis of Problem-Solving Progress.

    Science.gov (United States)

    Vendlinski, Terry

    This study used a computerized simulation and problem-solving tool along with artificial neural networks (ANN) as pattern recognizers to identify the common types of strategies high school and college undergraduate chemistry students would use to solve qualitative chemistry problems. Participants were 134 high school chemistry students who used…

  14. Identification of Strategies Used in Solving Transformational Geometry Problems.

    Science.gov (United States)

    Boulter, Douglas R.; Kirby, John R.

    1994-01-01

    Study investigated how to characterize students' transformational geometry problem-solving strategies, how strategies characterized particular subjects, and how strategy usage related to performance. Videotapes indicated problem-solving responses could be classified consistently, some students preferred holistic processing, some items readily…

  15. Problem Solving through an Optimization Problem in Geometry

    Science.gov (United States)

    Poon, Kin Keung; Wong, Hang-Chi

    2011-01-01

    This article adapts the problem-solving model developed by Polya to investigate and give an innovative approach to discuss and solve an optimization problem in geometry: the Regiomontanus Problem and its application to football. Various mathematical tools, such as calculus, inequality and the properties of circles, are used to explore and reflect…

  16. The Stages of Student Mathematical Imagination in Solving Mathematical Problems

    Science.gov (United States)

    Wibowo, Teguh; Sutawidjaja, Akbar; As'ari, Abdur Rahman; Sulandra, I. Made

    2017-01-01

    This research is a qualitative study that aimed to describe the stages of students mathematical imagination in solving mathematical problems. There are three kinds of mathematical imagination in solving mathematical problems, namely sensory mathematical imagination, creative mathematical imagination and recreative mathematical imagination.…

  17. Solving L-L Extraction Problems with Excel Spreadsheet

    Science.gov (United States)

    Teppaitoon, Wittaya

    2016-01-01

    This work aims to demonstrate the use of Excel spreadsheets for solving L-L extraction problems. The key to solving the problems successfully is to be able to determine a tie line on the ternary diagram where the calculation must be carried out. This enables the reader to analyze the extraction process starting with a simple operation, the…

  18. Facilitating Flexible Problem Solving: A Cognitive Load Perspective

    Science.gov (United States)

    Kalyuga, Slava; Renkl, Alexander; Paas, Fred

    2010-01-01

    The development of flexible, transferable problem-solving skills is an important aim of contemporary educational systems. Since processing limitations of our mind represent a major factor influencing any meaningful learning, the acquisition of flexible problem-solving skills needs to be based on known characteristics of our cognitive architecture…

  19. Adams Predictor-Corrector Systems for Solving Fuzzy Differential Equations

    Directory of Open Access Journals (Sweden)

    Dequan Shang

    2013-01-01

    Full Text Available A predictor-corrector algorithm and an improved predictor-corrector (IPC algorithm based on Adams method are proposed to solve first-order differential equations with fuzzy initial condition. These algorithms are generated by updating the Adams predictor-corrector method and their convergence is also analyzed. Finally, the proposed methods are illustrated by solving an example.

  20. A problem solving model for regulatory policy making

    NARCIS (Netherlands)

    Boer, A.; van Engers, T.; Sileno, G.; Wyner, A.; Benn, N.

    2011-01-01

    In this paper we discuss how the interests and field theory promoted by public administration as a stakeholder in policy argumentation, directly arise from its problem solving activities, using the framework for public administration problem solving we proposed in [1,2]. We propose that calls for

  1. Problem Solving and Collaboration Using Mobile Serious Games

    Science.gov (United States)

    Sanchez, Jaime; Olivares, Ruby

    2011-01-01

    This paper presents the results obtained with the implementation of a series of learning activities based on Mobile Serious Games (MSGs) for the development of problem solving and collaborative skills in Chilean 8th grade students. Three MSGs were developed and played by teams of four students in order to solve problems collaboratively. A…

  2. Using Systemic Problem Solving (SPS) to Assess Student ...

    African Journals Online (AJOL)

    However, systemic problem solving (SPS) can challenge students and probe higher cognitive skills like analysis, synthesis, and evaluation. Also, systemic problem solving (SPS) helps students to connect chemistry concepts, and facts and covers a wide range of intended learning outcomes (ILO,s). As an example, the type ...

  3. Problem Solving Treatment for Intellectually Disabled Sex Offenders

    Science.gov (United States)

    Nezu, Christine Maguth; Fiore, Alicia A.; Nezu, Arthur M.

    2006-01-01

    Over the past thirty years, Problem Solving Therapy (PST) has been shown to be an effective treatment for many different problems and patient populations (Nezu, 2004). Among its many clinical applications, PST interventions were developed for persons with intellectually disabilities (ID), where improving problem-solving skills led to adaptive…

  4. Working memory dysfunctions predict social problem solving skills in schizophrenia.

    Science.gov (United States)

    Huang, Jia; Tan, Shu-ping; Walsh, Sarah C; Spriggens, Lauren K; Neumann, David L; Shum, David H K; Chan, Raymond C K

    2014-12-15

    The current study aimed to examine the contribution of neurocognition and social cognition to components of social problem solving. Sixty-seven inpatients with schizophrenia and 31 healthy controls were administrated batteries of neurocognitive tests, emotion perception tests, and the Chinese Assessment of Interpersonal Problem Solving Skills (CAIPSS). MANOVAs were conducted to investigate the domains in which patients with schizophrenia showed impairments. Correlations were used to determine which impaired domains were associated with social problem solving, and multiple regression analyses were conducted to compare the relative contribution of neurocognitive and social cognitive functioning to components of social problem solving. Compared with healthy controls, patients with schizophrenia performed significantly worse in sustained attention, working memory, negative emotion, intention identification and all components of the CAIPSS. Specifically, sustained attention, working memory and negative emotion identification were found to correlate with social problem solving and 1-back accuracy significantly predicted the poor performance in social problem solving. Among the dysfunctions in schizophrenia, working memory contributed most to deficits in social problem solving in patients with schizophrenia. This finding provides support for targeting working memory in the development of future social problem solving rehabilitation interventions. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  5. expansion method for solving nonlinear space–time fractional ...

    Indian Academy of Sciences (India)

    2016-07-06

    Jul 6, 2016 ... -expansion method for solving fractional differential equations based on a fractional complex transform. We apply this method for solving space–time fractional Cahn–Allen equation and space–time fractional Klein–Gordon equation. The fractional derivatives are described in the sense of modified ...

  6. DEVELOPMENT OF TEACHING MATERIALS ALGEBRAIC EQUATION TO IMPROVE PROBLEM SOLVING

    Directory of Open Access Journals (Sweden)

    sri adi widodo

    2017-01-01

    Full Text Available Problem-solving skills are the basic capabilities of a person in solving a problem and that involve critical thinking, logical, and systematic. To solve a problem one-way necessary measures to solve the problem. Polya is one way to solve a mathematical problem. by developing teaching materials designed using the steps in solving problems Polya expected students could improve its ability to solve problems. In this first year, the goal of this study is to investigate the process of learning the hypothetical development of teaching materials. This study is a research & development. Procedure development research refers to research the development of Thiagarajan, Semmel & Semmel ie 4-D. Model development in the first year is define, design, and development. The collection of data for the assessment of teaching materials algebra equations conducted by the expert by filling the validation sheet. Having examined the materials of algebraic equations in the subject of numerical methods, reviewing the curriculum that is aligned with KKNI, and formulates learning outcomes that formed the conceptual teaching material on the material algebraic equations. From the results of expert assessment team found that the average ratings of teaching materials in general algebraic equation of 4.38 with a very good category. The limited test needs to be done to see effectiveness teaching materials on problem-solving skills in students who are taking courses numerical methods

  7. Students' Epistemological Framing in Quantum Mechanics Problem Solving

    Science.gov (United States)

    Modir, Bahar; Thompson, John D.; Sayre, Eleanor C.

    2017-01-01

    Students' difficulties in quantum mechanics may be the result of unproductive framing and not a fundamental inability to solve the problems or misconceptions about physics content. We observed groups of students solving quantum mechanics problems in an upper-division physics course. Using the lens of epistemological framing, we investigated four…

  8. Dimensional Analysis and Qualitative Methods in Problem Solving

    Science.gov (United States)

    Pescetti, D.

    2008-01-01

    The primary application of dimensional analysis (DA) is in problem solving. Typically, the problem description indicates that a physical quantity Y(the unknown) is a function f of other physical quantities A[subscript 1], ..., A[subscript n] (the data). We propose a qualitative problem-solving procedure which consists of a parallel decomposition…

  9. Measuring Problem Solving Skills in Plants vs. Zombies 2

    Science.gov (United States)

    Shute, Valerie J.; Moore, Gregory R.; Wang, Lubin

    2015-01-01

    We are using stealth assessment, embedded in "Plants vs. Zombies 2," to measure middle-school students' problem solving skills. This project started by developing a problem solving competency model based on a thorough review of the literature. Next, we identified relevant in-game indicators that would provide evidence about students'…

  10. Structured collaboration versus individual learning in solving physics problems

    NARCIS (Netherlands)

    Harskamp, Egbert; Ding, Ning

    2006-01-01

    The research issue in this study is how to structure collaborative learning so that it improves solving physics problems more than individual learning. Structured collaborative learning has been compared with individual learning environments with Schoenfeld's problem-solving episodes. Students took

  11. Teaching problem-solving competency in Business Studies at ...

    African Journals Online (AJOL)

    10218122

    solving can broadly be conceptualised as a process that includes five steps. During the teaching of problem-solving, the teacher should focus on the following steps that have to be executed by the learners (Crebert et al., 2011:10-. 11; Tull, 2012:1-2), ...

  12. Extricating Justification Scheme Theory in Middle School Mathematical Problem Solving

    Science.gov (United States)

    Matteson, Shirley; Capraro, Mary Margaret; Capraro, Robert M.; Lincoln, Yvonna S.

    2012-01-01

    Twenty middle grades students were interviewed to gain insights into their reasoning about problem-solving strategies using a Problem Solving Justification Scheme as our theoretical lens and the basis for our analysis. The scheme was modified from the work of Harel and Sowder (1998) making it more broadly applicable and accounting for research…

  13. Problem Solving Ability Of Students Mathematics In Problem Based Learning

    Directory of Open Access Journals (Sweden)

    Nurdiana Siregar

    2017-12-01

    Full Text Available The objective of this study were to determine the increasing of students’ problem solving ability and self confidence after the implementation of problem based learning and to see the interaction between gender and learning to increas problem solving ability and students’ self confidence. The method kuantitaif with the design of this research was quasi-experimental with factorial design is 22. There were 73 students as the sample of this study containing 36 students in grade VIII-5 and 37 students in grade VIII-6. The instruments of this students were test of problem solving and self confidence scale. The data were analyzed by applying two way analysis of variance (ANOVA. The result showed that (1 The increasing of students’ problem solving ability taught by using problem based learning was higher than that taught by using conventional learning (2 There was no interaction between gender and learning with increasing of students’ problem solving ability.

  14. Effectiveness of discovery learning model on mathematical problem solving

    Science.gov (United States)

    Herdiana, Yunita; Wahyudin, Sispiyati, Ririn

    2017-08-01

    This research is aimed to describe the effectiveness of discovery learning model on mathematical problem solving. This research investigate the students' problem solving competency before and after learned by using discovery learning model. The population used in this research was student in grade VII in one of junior high school in West Bandung Regency. From nine classes, class VII B were randomly selected as the sample of experiment class, and class VII C as control class, which consist of 35 students every class. The method in this research was quasi experiment. The instrument in this research is pre-test, worksheet and post-test about problem solving of mathematics. Based on the research, it can be conclude that the qualification of problem solving competency of students who gets discovery learning model on level 80%, including in medium category and it show that discovery learning model effective to improve mathematical problem solving.

  15. Social problem solving ability predicts mental health among undergraduate students.

    Science.gov (United States)

    Ranjbar, Mansour; Bayani, Ali Asghar; Bayani, Ali

    2013-11-01

    The main objective of this study was predicting student's mental health using social problem solving- ability. In this correlational. descriptive study, 369 (208 female and 161 male) from, Mazandaran University of Medical Science were selected through stratified random sampling method. In order to collect the data, the social problem solving inventory-revised and general health questionnaire were used. Data were analyzed through SPSS-19, Pearson's correlation, t test, and stepwise regression analysis. Data analysis showed significant relationship between social problem solving ability and mental health (P Social problem solving ability was significantly associated with the somatic symptoms, anxiety and insomnia, social dysfunction and severe depression (P social problem solving ability and mental health.

  16. How Do They Solve It? An Insight into the Learner's Approach to the Mechanism of Physics Problem Solving

    Science.gov (United States)

    Hegde, Balasubrahmanya; Meera, B. N.

    2012-01-01

    A perceived difficulty is associated with physics problem solving from a learner's viewpoint, arising out of a multitude of reasons. In this paper, we have examined the microstructure of students' thought processes during physics problem solving by combining the analysis of responses to multiple-choice questions and semistructured student…

  17. Does Solving Insight-Based Problems Differ from Solving Learning-Based Problems? Some Evidence from an ERP Study

    Science.gov (United States)

    Leikin, Roza; Waisman, Ilana; Leikin, Mark

    2016-01-01

    We asked: "What are the similarities and differences in mathematical processing associated with solving learning-based and insight-based problems?" To answer this question, the ERP research procedure was employed with 69 male adolescent subjects who solved specially designed insight-based and learning-based tests. Solutions of…

  18. How indirect supportive digital help during and after solving physics problems can improve problem-solving abilities

    NARCIS (Netherlands)

    Pol, Henk J.; Harskamp, Egbert G.; Suhre, Cor J. M.; Goedhart, Martin J.

    This study investigates the effectiveness of computer-delivered hints in relation to problem-solving abilities in two alternative indirect instruction schemes. In one instruction scheme, hints are available to students immediately after they are given a new problem to solve as well as after they

  19. Problem-solving skills in high school biology: The effectiveness of the IMMEX problem-solving assessment software

    Science.gov (United States)

    Palacio-Cayetano, Joycelin

    "Problem-solving through reflective thinking should be both the method and valuable outcome of science instruction in America's schools" proclaimed John Dewey (Gabel, 1995). If the development of problem-solving is a primary goal of science education, more problem-solving opportunities must be an integral part of K-16 education. To examine the effective use of technology in developing and assessing problem-solving skills, a problem-solving authoring, learning, and assessment software, the UCLA IMMEX Program-Interactive Multimedia Exercises-was investigated. This study was a twenty-week quasi-experimental study that was implemented as a control-group time series design among 120 tenth grade students. Both the experimental group (n = 60) and the control group (n = 60) participated in a problem-based learning curriculum; however, the experimental group received regular intensive experiences with IMMEX problem-solving and the control group did not. Problem-solving pretest and posttest were administered to all students. The instruments used were a 35-item Processes of Biological Inquiry Test and an IMMEX problem-solving assessment test, True Roots. Students who participated in the IMMEX Program achieved significant (p problem-solving skills on both problem-solving assessment instruments. This study provided evidence that IMMEX software is highly efficient in evaluating salient elements of problem-solving. Outputs of students' problem-solving strategies revealed that unsuccessful problem solvers primarily used the following four strategies: (1) no data search strategy, students simply guessed; (2) limited data search strategy leading to insufficient data and premature closing; (3) irrelevant data search strategy, students focus in areas bearing no substantive data; and (4) extensive data search strategy with inadequate integration and analysis. On the contrary, successful problem solvers used the following strategies; (1) focused search strategy coupled with the ability

  20. Social problem solving and coping skills of medical students

    Directory of Open Access Journals (Sweden)

    Emel Yigit

    2016-12-01

    Full Text Available Purpose: The aim of this study was to evaluate medical students' social problem solving and coping skills. Material and Methods: In this correlational descriptive study, data were gathered from 457 medical students. Social Problem Solving Inventory and Social Problem Coping Behaviours Inventory were used. Results: The most preferred activities when the students face a problem were talking with friends (87.1%, talking with special persons (85.4%, sleeping (82.6%, talking with family members (81.6%, and eating (79.8%. The ratio of the behaviors that can be deemed risky were exhibiting aggressive and violent behaviors (18.9%, drinking alcohol (18.7%, smoking (17.6%, playing games of chance (16.9%, and using substance (3.8%. There was a positive relationship between total scores of Social Problem Solving Inventory and Social Problem Coping Behaviours Inventory. It is found that immature social problem solving ability has increased the risk of unfavourable behaviours by 3.1 fold. Conclusion: Social problem solving ability is significantly correlated with coping behaviours and may predict it. Medical students who are the doctors and the role models of the future need to develop their social problem solving skills in addition to clinical problem solving skills. [Cukurova Med J 2016; 41(4.000: 721-731

  1. Problem-solving phase transitions during team collaboration

    DEFF Research Database (Denmark)

    Wiltshire, Travis; Butner, Jonathan E.; Fiore, Stephen M.

    2018-01-01

    Multiple theories of problem-solving hypothesize that there are distinct qualitative phases exhibited during effective problem-solving. However, limited research has attempted to identify when transitions between phases occur. We integrate theory on collaborative problem-solving (CPS) with dynami......Multiple theories of problem-solving hypothesize that there are distinct qualitative phases exhibited during effective problem-solving. However, limited research has attempted to identify when transitions between phases occur. We integrate theory on collaborative problem-solving (CPS......) with dynamical systems theory suggesting that when a system is undergoing a phase transition it should exhibit a peak in entropy and that entropy levels should also relate to team performance. Communications from 40 teams that collaborated on a complex problem were coded for occurrence of problem......-solving processes. We applied a sliding window entropy technique to each team's communications and specified criteria for (a) identifying data points that qualify as peaks and (b) determining which peaks were robust. We used multilevel modeling, and provide a qualitative example, to evaluate whether phases exhibit...

  2. Internet computer coaches for introductory physics problem solving

    Science.gov (United States)

    Xu Ryan, Qing

    The ability to solve problems in a variety of contexts is becoming increasingly important in our rapidly changing technological society. Problem-solving is a complex process that is important for everyday life and crucial for learning physics. Although there is a great deal of effort to improve student problem solving skills throughout the educational system, national studies have shown that the majority of students emerge from such courses having made little progress toward developing good problem-solving skills. The Physics Education Research Group at the University of Minnesota has been developing Internet computer coaches to help students become more expert-like problem solvers. During the Fall 2011 and Spring 2013 semesters, the coaches were introduced into large sections (200+ students) of the calculus based introductory mechanics course at the University of Minnesota. This dissertation, will address the research background of the project, including the pedagogical design of the coaches and the assessment of problem solving. The methodological framework of conducting experiments will be explained. The data collected from the large-scale experimental studies will be discussed from the following aspects: the usage and usability of these coaches; the usefulness perceived by students; and the usefulness measured by final exam and problem solving rubric. It will also address the implications drawn from this study, including using this data to direct future coach design and difficulties in conducting authentic assessment of problem-solving.

  3. Multistage Spectral Relaxation Method for Solving the Hyperchaotic Complex Systems

    Directory of Open Access Journals (Sweden)

    Hassan Saberi Nik

    2014-01-01

    Full Text Available We present a pseudospectral method application for solving the hyperchaotic complex systems. The proposed method, called the multistage spectral relaxation method (MSRM is based on a technique of extending Gauss-Seidel type relaxation ideas to systems of nonlinear differential equations and using the Chebyshev pseudospectral methods to solve the resulting system on a sequence of multiple intervals. In this new application, the MSRM is used to solve famous hyperchaotic complex systems such as hyperchaotic complex Lorenz system and the complex permanent magnet synchronous motor. We compare this approach to the Runge-Kutta based ode45 solver to show that the MSRM gives accurate results.

  4. Systematic Problem Solving in Production: The NAX Approach

    DEFF Research Database (Denmark)

    Axelsdottir, Aslaug; Nygaard, Martin; Edwards, Kasper

    2017-01-01

    This paper outlines the NAX problem solving approach developed by a group of problem solving experts at a large Danish Producer of medical equipment. The company, “Medicmeter” is one of Denmark’s leading companies when it comes to lean and it has developed a strong problem solving culture. The main...... steps of the approach are to extensively gather direct detailed process knowledge at the actual process, assemble a team that systematically builds on each other ideas, apply team thinking in a structured way to get a rapid and very deep understanding of the problem, and conducting a structured...

  5. An approach to numerically solving the Poisson equation

    Science.gov (United States)

    Feng, Zhichen; Sheng, Zheng-Mao

    2015-06-01

    We introduce an approach for numerically solving the Poisson equation by using a physical model, which is a way to solve a partial differential equation without the finite difference method. This method is especially useful for obtaining the solutions in very many free-charge neutral systems with open boundary conditions. It can be used for arbitrary geometry and mesh style and is more efficient comparing with the widely-used iterative algorithm with multigrid methods. It is especially suitable for parallel computing. This method can also be applied to numerically solving other partial differential equations whose Green functions exist in analytic expression.

  6. Solving Complex Problems to Create Charter Extension Options

    DEFF Research Database (Denmark)

    Tippmann, Esther; Nell, Phillip Christopher

    This study examines subsidiary-driven problem solving processes and their potential to create advanced solutions for charter extension options. Problem solving theory suggests that biases in problem formulation and solution search can confine problem solving potential. We thus argue that balanced...... solution search, or activities to reconcile the need for some solution features to be locally-tailored while others can be internationally standardized, mediates the relationships between problem complexity/headquarters involvement and the capacity to create advanced solutions. An analysis of 67 projects...

  7. Dimensional analysis and qualitative methods in problem solving: II

    International Nuclear Information System (INIS)

    Pescetti, D

    2009-01-01

    We show that the underlying mathematical structure of dimensional analysis (DA), in the qualitative methods in problem-solving context, is the algebra of the affine spaces. In particular, we show that the qualitative problem-solving procedure based on the parallel decomposition of a problem into simple special cases yields the new original mathematical concepts of special points and special representations of affine spaces. A qualitative problem-solving algorithm piloted by the mathematics of DA is illustrated by a set of examples.

  8. Problem solving performance and learning strategies of undergraduate students who solved microbiology problems using IMMEX educational software

    Science.gov (United States)

    Ebomoyi, Josephine Itota

    The objectives of this study were as follows: (1) Determine the relationship between learning strategies and performance in problem solving, (2) Explore the role of a student's declared major on performance in problem solving, (3) Understand the decision making process of high and low achievers during problem solving. Participants (N = 65) solved problems using the Interactive multimedia exercise (IMMEX) software. All participants not only solved "Microquest," which focuses on cellular processes and mode of action of antibiotics, but also "Creeping Crud," which focuses on the cause, origin and transmission of diseases. Participants also responded to the "Motivated Strategy Learning Questionnaire" (MSLQ). Hierarchical multiple regression was used for analysis with GPA (Gracie point average) as a control. There were 49 (78.6%) that successfully solved "Microquest" while 52 (82.5%) successfully solved "Creeping Crud". Metacognitive self regulation strategy was significantly (p High achievers used fewer steps, and had more focused approach than low achievers. Common strategies and attributes included metacognitive skills, writing to keep track, using prior knowledge. Others included elements of frustration/confusion and self-esteem problems. The implications for educational and relevance to real life situations are discussed.

  9. Mathematical Thinking and Creativity through Mathematical Problem Posing and Solving

    Directory of Open Access Journals (Sweden)

    María F. Ayllón

    2016-04-01

    Full Text Available This work shows the relationship between the development of mathematical thinking and creativity with mathematical problem posing and solving. Creativity and mathematics are disciplines that do not usually appear together. Both concepts constitute complex processes sharing elements, such as fluency (number of ideas, flexibility (range of ideas, novelty (unique idea and elaboration (idea development. These factors contribute, among others, to the fact that schoolchildren are competent in mathematics. The problem solving and posing are a very powerful evaluation tool that shows the mathematical reasoning and creative level of a person. Creativity is part of the mathematics education and is a necessary ingredient to perform mathematical assignments. This contribution presents some important research works about problem posing and solving related to the development of mathematical knowledge and creativity. To that end, it is based on various beliefs reflected in the literature with respect to notions of creativity, problem solving and posing.

  10. Problem solving in foundation engineering using foundationPro

    CERN Document Server

    Yamin, Mohammad

    2016-01-01

    This book is at once a supplement to traditional foundation engineering textbooks and an independent problem-solving learning tool. The book is written primarily for university students majoring in civil or construction engineering taking foundation analysis and design courses to encourage them to solve design problems. Its main aim is to stimulate problem solving capability and foster self-directed learning. It also explains the use of the foundationPro software, available at no cost, and includes a set of foundation engineering applications. Taking a unique approach, Dr. Yamin summarizes the general step-by-step procedure to solve various foundation engineering problems, illustrates traditional applications of these steps with longhand solutions, and presents the foundationPro solutions. The special structure of the book allows it to be used in undergraduate and graduate foundation design and analysis courses in civil and construction engineering. The book stands as valuable resource for students, faculty, ...

  11. Examining Multiscale Movement Coordination in Collaborative Problem Solving

    DEFF Research Database (Denmark)

    Wiltshire, Travis; Steffensen, Sune Vork

    2017-01-01

    During collaborative problem solving (CPS), coordination occurs at different spatial and temporal scales. This multiscale coordination should, at least on some scales, play a functional role in facilitating effective collaboration outcomes. To evaluate this, we conducted a study of computer...

  12. Genetic Algorithm for Solving Simple Mathematical Equality Problem

    OpenAIRE

    Hermawanto, Denny

    2013-01-01

    This paper explains genetic algorithm for novice in this field. Basic philosophy of genetic algorithm and its flowchart are described. Step by step numerical computation of genetic algorithm for solving simple mathematical equality problem will be briefly explained

  13. Final report [on solving the multigroup diffusion equations

    International Nuclear Information System (INIS)

    Birkhoff, G.

    1975-01-01

    Progress achieved in the development of variational methods for solving the multigroup neutron diffusion equations is described. An appraisal is made of the extent to which improved variational methods could advantageously replace difference methods currently used

  14. Planning under uncertainty solving large-scale stochastic linear programs

    Energy Technology Data Exchange (ETDEWEB)

    Infanger, G. [Stanford Univ., CA (United States). Dept. of Operations Research]|[Technische Univ., Vienna (Austria). Inst. fuer Energiewirtschaft

    1992-12-01

    For many practical problems, solutions obtained from deterministic models are unsatisfactory because they fail to hedge against certain contingencies that may occur in the future. Stochastic models address this shortcoming, but up to recently seemed to be intractable due to their size. Recent advances both in solution algorithms and in computer technology now allow us to solve important and general classes of practical stochastic problems. We show how large-scale stochastic linear programs can be efficiently solved by combining classical decomposition and Monte Carlo (importance) sampling techniques. We discuss the methodology for solving two-stage stochastic linear programs with recourse, present numerical results of large problems with numerous stochastic parameters, show how to efficiently implement the methodology on a parallel multi-computer and derive the theory for solving a general class of multi-stage problems with dependency of the stochastic parameters within a stage and between different stages.

  15. The Association ofDRD2with Insight Problem Solving.

    Science.gov (United States)

    Zhang, Shun; Zhang, Jinghuan

    2016-01-01

    Although the insight phenomenon has attracted great attention from psychologists, it is still largely unknown whether its variation in well-functioning human adults has a genetic basis. Several lines of evidence suggest that genes involved in dopamine (DA) transmission might be potential candidates. The present study explored for the first time the association of dopamine D2 receptor gene ( DRD2 ) with insight problem solving. Fifteen single-nucleotide polymorphisms (SNPs) covering DRD2 were genotyped in 425 unrelated healthy Chinese undergraduates, and were further tested for association with insight problem solving. Both single SNP and haplotype analysis revealed several associations of DRD2 SNPs and haplotypes with insight problem solving. In conclusion, the present study provides the first evidence for the involvement of DRD2 in insight problem solving, future studies are necessary to validate these findings.

  16. Retention of Problem-Solving Performance in School Contexts.

    Science.gov (United States)

    Ross, John A.; Maynes, Florence J.

    1985-01-01

    This article reports data on the retention of problem-solving performance after one month, four months, and six months, in three separate studies conducted in school settings involving a variety of grades and subjects. (Author/LMO)

  17. Spreadsheet-Enhanced Problem Solving in Context as Modeling

    Directory of Open Access Journals (Sweden)

    Sergei Abramovich

    2003-07-01

    development through situated mathematical problem solving. Modeling activities described in this paper support the epistemological position regarding the interplay that exists between the development of mathematical concepts and available methods of calculation. The spreadsheet used is Microsoft Excel 2001

  18. Solving the Stokes problem on a massively parallel computer

    DEFF Research Database (Denmark)

    Axelsson, Owe; Barker, Vincent A.; Neytcheva, Maya

    2001-01-01

    We describe a numerical procedure for solving the stationary two‐dimensional Stokes problem based on piecewise linear finite element approximations for both velocity and pressure, a regularization technique for stability, and a defect‐correction technique for improving accuracy. Eliminating...... boundary value problem for each velocity component, are solved by the conjugate gradient method with a preconditioning based on the algebraic multi‐level iteration (AMLI) technique. The velocity is found from the computed pressure. The method is optimal in the sense that the computational work...... the velocity unknowns from the algebraic system yields a symmetric positive semidefinite system for pressure which is solved by an inner‐outer iteration. The outer iterations consist of the unpreconditioned conjugate gradient method. The inner iterations, each of which corresponds to solving an elliptic...

  19. Solving the brachistochrone and other variational problems with soap films

    Science.gov (United States)

    Criado, C.; Alamo, N.

    2010-12-01

    We solve the problem of the brachistochrone and other variational problems with the help of the soap films that are formed between two suitable surfaces. We also discuss the connection between some variational problems of dynamics, statics, optics, and elasticity.

  20. The Intermediate Impossible: A Prewriting Activity for Creative Problem Solving.

    Science.gov (United States)

    Karloff, Kenneth

    1985-01-01

    Adapts Edward de Bono's "Intermediate Impossible" strategy--for considering ideas that normally would be discarded as stepping-stones to new ideas--for use as a prewriting activity to enhance creative problem solving. (HTH)

  1. The Environmental Justice Collaborative Problem-Solving Cooperative Agreement Program

    Science.gov (United States)

    The Environmental Justice Collaborative Problem-Solving (CPS) Cooperative Agreement Program provides financial assistance to eligible organizations working on or planning to work on projects to address local environmental and/or public health issues

  2. Stepping out of history : Mindfulness improves insight problem solving

    NARCIS (Netherlands)

    Ostafin, B.D.; Kassman, K.T.

    Insight problem solving is hindered by automated verbal-conceptual processes. Because mindfulness meditation training aims at "nonconceptual awareness" which involves a reduced influence of habitual verbal-conceptual processes on the interpretation of ongoing experience, mindfulness may facilitate

  3. Solving Variable Coefficient Fourth-Order Parabolic Equation by ...

    African Journals Online (AJOL)

    In this paper, a Modified initial guess Variational Iteration Method (MigVIM) is used to solve a non-homogeneous variable coefficient fourth order parabolic partial differential equations. The new method shows rapid convergence to the exact solution.

  4. An Efficient Algorithm for Solving the Telegraph Equation ...

    African Journals Online (AJOL)

    Abstract. In this work, we propose a numerical scheme to solve telegraph equations using modified variational iteration method. The numerical results are compared with analytical solutions to confirm the efficiency of the method.

  5. Book Review: Solving Language Problems: From General to ...

    African Journals Online (AJOL)

    Abstract. Book Title: Solving Language Problems: From General to Applied Linguistics. Book Author: R.R.K. Hartmann (Ed.) 1st edition 1996, vi + 298 pp. ISBN 0-85989-484-3. Exeter: University of Exeter Press.

  6. Using Evolutionary Computation to Solve the Economic Load Dispatch Problem

    Directory of Open Access Journals (Sweden)

    Samir SAYAH

    2008-06-01

    Full Text Available This paper reports on an evolutionary algorithm based method for solving the economic load dispatch (ELD problem. The objective is to minimize the nonlinear function, which is the total fuel cost of thermal generating units, subject to the usual constraints.The IEEE 30 bus test system was used for testing and validation purposes. The results obtained demonstrate the effectiveness of the proposed method for solving the economic load dispatch problem.

  7. Variational iteration method for solving coupled-KdV equations

    International Nuclear Information System (INIS)

    Assas, Laila M.B.

    2008-01-01

    In this paper, the He's variational iteration method is applied to solve the non-linear coupled-KdV equations. This method is based on the use of Lagrange multipliers for identification of optimal value of a parameter in a functional. This technique provides a sequence of functions which converge to the exact solution of the coupled-KdV equations. This procedure is a powerful tool for solving coupled-KdV equations

  8. Scaffolding for solving problem in static fluid: A case study

    Science.gov (United States)

    Koes-H, Supriyono; Muhardjito, Wijaya, Charisma P.

    2018-01-01

    Problem solving is one of the basic abilities that should be developed from learning physics. However, students still face difficulties in the process of non-routine problem-solving. Efforts are necessary to be taken in order to identify such difficulties and the solutions to solve them. An effort in the form of a diagnosis of students' performance in problem solving can be taken to identify their difficulties, and various instructional scaffolding supports can be utilized to eliminate the difficulties. This case study aimed to describe the students' difficulties in solving static fluid problems and the effort to overcome such difficulties through different scaffolding supports. The research subjects consisted of four 10-grade students of (Public Senior High School) SMAN 4 Malang selected by purposive sampling technique. The data of students' difficulties were collected via think-aloud protocol implemented on students' performance in solving non-routine static fluid problems. Subsequently, combined scaffolding supports were given to the students based on their particular difficulties. The research findings pointed out that there were several conceptual difficulties discovered from the students when solving static fluid problems, i.e. the use of buoyancy force formula, determination of all forces acting on a plane in a fluid, the resultant force on a plane in a fluid, and determination of a plane depth in a fluid. An effort that can be taken to overcome such conceptual difficulties is providing a combination of some appropriate scaffolding supports, namely question prompts with specific domains, simulation, and parallel modeling. The combination can solve students' lack of knowledge and improve their conceptual understanding, as well as help them to find solutions by linking the problems with their prior knowledge. According to the findings, teachers are suggested to diagnose the students' difficulties so that they can provide an appropriate combination of

  9. An Approach for Solving Linear Fractional Programming Problems

    OpenAIRE

    Andrew Oyakhobo Odior

    2012-01-01

    Linear fractional programming problems are useful tools in production planning, financial and corporate planning, health care and hospital planning and as such have attracted considerable research interest. The paper presents a new approach for solving a fractional linear programming problem in which the objective function is a linear fractional function, while the constraint functions are in the form of linear inequalities. The approach adopted is based mainly upon solving the problem algebr...

  10. The art and science of participative problem solving

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    In this paper we will document that real-life problem solving in complex situations demands both rational (scientific) and intuitive (artistic) thinking. First, the concepts of art and science will be discussed; differences and similarities will be enhanced. Thereafter the concept of group problem...... solving facilitation both as science and art will be presented. A case study related to examinations planning will be discussed to illustrate the main concepts in practice. In addition, other cases studies will also be shortly presented....

  11. Creative and Participative Problem Solving - The Art and the Science

    DEFF Research Database (Denmark)

    Vidal, Rene Victor Valqui

    This book collects my experiences as a facilitator for many different communities and organizations and as a teacher at the Technical University of Denmark for the courses Creativity and Problem Solving and Systemic Operational Research. Several of the chapters has been used in my lecturing...... activities in Denmark and abroad. The target groups for this book are people and professionals from communities, organizations, and ad-hoc groups facing problematic situations that have to be solved in an innovative way....

  12. Using a genetic algorithm to solve fluid-flow problems

    Energy Technology Data Exchange (ETDEWEB)

    Pryor, R.J. (Sandia National Lab., Albuquerque, NM (USA))

    1990-06-01

    Genetic algorithms are based on the mechanics of the natural selection and natural genetics processes. These algorithms are finding increasing application to a wide variety of engineering optimization and machine learning problems. In this paper, the authors demonstrate the use of a genetic algorithm to solve fluid flow problems. Specifically, the authors use the algorithm to solve the one-dimensional flow equations for a pipe.

  13. English-language learners’ problem solving in Spanish versus English

    OpenAIRE

    Ambrose, Rebecca; Molina, Marta

    2010-01-01

    To explore the role of language in English Language Learners (ELLs)´ problem solving, we compare the performance of a group of Latino first graders when working in Spanish and in English on two equivalent sets of story problems. We contrast our results with others from previous studies with bilingual and monolinguals children by focusing on students´ performance in problems with the same semantic structure. This comparison leads us to discuss some factors influencing students´ problem solving...

  14. Optimal calculational schemes for solving multigroup photon transport problem

    International Nuclear Information System (INIS)

    Dubinin, A.A.; Kurachenko, Yu.A.

    1987-01-01

    A scheme of complex algorithm for solving multigroup equation of radiation transport is suggested. The algorithm is based on using the method of successive collisions, the method of forward scattering and the spherical harmonics method, and is realized in the FORAP program (FORTRAN, BESM-6 computer). As an example the results of calculating reactor photon transport in water are presented. The considered algorithm being modified may be used for solving neutron transport problems

  15. A new Newton-like method for solving nonlinear equations.

    Science.gov (United States)

    Saheya, B; Chen, Guo-Qing; Sui, Yun-Kang; Wu, Cai-Ying

    2016-01-01

    This paper presents an iterative scheme for solving nonline ar equations. We establish a new rational approximation model with linear numerator and denominator which has generalizes the local linear model. We then employ the new approximation for nonlinear equations and propose an improved Newton's method to solve it. The new method revises the Jacobian matrix by a rank one matrix each iteration and obtains the quadratic convergence property. The numerical performance and comparison show that the proposed method is efficient.

  16. Social Problem Solving Ability Predicts Mental Health Among Undergraduate Students

    OpenAIRE

    Ranjbar, Mansour; Bayani, Ali Asghar; Bayani, Ali

    2013-01-01

    Background : The main objective of this study was predicting student′s mental health using social problem solving- ability . Methods : In this correlational- descriptive study, 369 (208 female and 161 male) from, Mazandaran University of Medical Science were selected through stratified random sampling method. In order to collect the data, the social problem solving inventory-revised and general health questionnaire were used. Data were analyzed through SPSS-19, Pearson′s correlation, t tes...

  17. Problem solving teaching practices: Observer and teacher's view

    OpenAIRE

    Felmer , Patricio; Perdomo-Díaz , Josefa; Giaconi , Valentina; Espinoza , Carmen ,

    2015-01-01

    International audience; In this article, we report on an exploratory study on teaching practices related to problem solving of a group of 29 novel secondary mathematics teachers. For this purpose, two independent instruments were designed, the first one is based on lesson observations, and the second one is a questionnaire answered by teachers about their teaching practices while working on non-routine problem solving with their students. For each instrument, we perform a statistical analysis...

  18. Using a genetic algorithm to solve fluid-flow problems

    International Nuclear Information System (INIS)

    Pryor, R.J.

    1990-01-01

    Genetic algorithms are based on the mechanics of the natural selection and natural genetics processes. These algorithms are finding increasing application to a wide variety of engineering optimization and machine learning problems. In this paper, the authors demonstrate the use of a genetic algorithm to solve fluid flow problems. Specifically, the authors use the algorithm to solve the one-dimensional flow equations for a pipe

  19. University Physics As a Second Language: Mastering Problem Solving

    Science.gov (United States)

    Barrett, Tom

    2005-09-01

    Get a better grade in Physics Solving physics problems can be challenging at times. But with hard work and the right study tools, you can learn the language of physics and get the grade you want. With Tom Barrett's University Physics as a Second Language(TM): Mastering Problem Solving, you'll be able to better understand fundamental physics concepts, solve a variety of problems, and focus on what you need to know to succeed. Here's how you can get a better grade in physics: Understand the basic concepts University Physics as a Second Language(TM) focuses on selected topics in calculus-based physics to give you a solid foundation. Tom Barrett explains these topics in clear, easy-to-understand language. Break problems down into simple steps University Physics as a Second Language(TM) teaches you to approach problems more efficiently and effectively. You'll learn how to recognize common patterns in physics problems, break problems down into manageable steps, and apply appropriate techniques. The book takes you step-by-step through the solutions to numerous examples. Improve your problem-solving skills University Physics as a Second Language(TM) will help you develop the skills you need to solve a variety of problem types. You'll learn timesaving problem-solving strategies that will help you focus your efforts, as well as how to avoid potential pitfalls.

  20. Self-affirmation improves problem-solving under stress.

    Directory of Open Access Journals (Sweden)

    J David Creswell

    Full Text Available High levels of acute and chronic stress are known to impair problem-solving and creativity on a broad range of tasks. Despite this evidence, we know little about protective factors for mitigating the deleterious effects of stress on problem-solving. Building on previous research showing that self-affirmation can buffer stress, we tested whether an experimental manipulation of self-affirmation improves problem-solving performance in chronically stressed participants. Eighty undergraduates indicated their perceived chronic stress over the previous month and were randomly assigned to either a self-affirmation or control condition. They then completed 30 difficult remote associate problem-solving items under time pressure in front of an evaluator. Results showed that self-affirmation improved problem-solving performance in underperforming chronically stressed individuals. This research suggests a novel means for boosting problem-solving under stress and may have important implications for understanding how self-affirmation boosts academic achievement in school settings.

  1. Problem Solving and Critical Thinking Skills of Undergraduate Nursing Students

    Directory of Open Access Journals (Sweden)

    Yalçın KANBAY

    2013-12-01

    Full Text Available Due to the fact that critical thinking and problem solving skills are essential components of educational and social lives of individuals, this present study which investigate critical thinking and problem solving skills of undergraduate students of nursing was planned. This is a descriptive study. The study population consisted of undergraduate nursing students of a university during the 2011-2012 academic year. Any specific sampling method was not determined and only the voluntary students was enrolled in the study . Several participants were excluded due to incomplete questionnaires, and eventually a total of 231 nursing students were included in the final sampling. Socio Demographic Features Data Form and the California Critical Thinking Disposition Scale and Problem Solving Inventory were used for data collection. The mean age of 231 subjects (148 girls, 83 boys was 21.34. The mean score of critical thinking was 255.71 for the first-grade, 255.57 for the second-grade, 264.73 for the third-grade, and 256.468 for the forth-grade students. The mean score of critical thinking was determined as 257.41 for the sample, which can be considered as an average value. Although there are mean score differences of critical thinking between the classes , they were not statistically significant (p> 0.05. With regard to the mean score of problem solving, the first-grade students had 92.86, the second-grade students had 94. 29, the third-grade students had 87.00, and the forth-grade students had 92.87. The mean score of problem solving was determined as 92.450 for the sample. Although there are differences between the classes in terms of mean scores of problem solving, it was not found statistically significant (p> 0.05. In this study, statistically significant correlation could not be identified between age and critical thinking skills of the subjects (p>0.05. However, a negative correlation was identified at low levels between critical thinking skills and

  2. Review on solving the forward problem in EEG source analysis

    Directory of Open Access Journals (Sweden)

    Vergult Anneleen

    2007-11-01

    Full Text Available Abstract Background The aim of electroencephalogram (EEG source localization is to find the brain areas responsible for EEG waves of interest. It consists of solving forward and inverse problems. The forward problem is solved by starting from a given electrical source and calculating the potentials at the electrodes. These evaluations are necessary to solve the inverse problem which is defined as finding brain sources which are responsible for the measured potentials at the EEG electrodes. Methods While other reviews give an extensive summary of the both forward and inverse problem, this review article focuses on different aspects of solving the forward problem and it is intended for newcomers in this research field. Results It starts with focusing on the generators of the EEG: the post-synaptic potentials in the apical dendrites of pyramidal neurons. These cells generate an extracellular current which can be modeled by Poisson's differential equation, and Neumann and Dirichlet boundary conditions. The compartments in which these currents flow can be anisotropic (e.g. skull and white matter. In a three-shell spherical head model an analytical expression exists to solve the forward problem. During the last two decades researchers have tried to solve Poisson's equation in a realistically shaped head model obtained from 3D medical images, which requires numerical methods. The following methods are compared with each other: the boundary element method (BEM, the finite element method (FEM and the finite difference method (FDM. In the last two methods anisotropic conducting compartments can conveniently be introduced. Then the focus will be set on the use of reciprocity in EEG source localization. It is introduced to speed up the forward calculations which are here performed for each electrode position rather than for each dipole position. Solving Poisson's equation utilizing FEM and FDM corresponds to solving a large sparse linear system. Iterative

  3. [Methods for teaching problem-solving in medical schools].

    Science.gov (United States)

    Shumway, J M; Vargas, M E; Heller, L E

    1984-01-01

    The need to include in the medical curriculum instructional activities to promote the development of problem-solving abilities has been asserted at the national and international levels. In research on the mental process involved in the solution of problems in medicine, problem-solving has been defined as a hypothetical-deductive activity engaged in by experienced physicians, in which the early generation of hypotheses influences the subsequent gathering of information. This article comments briefly on research on the mental process by which medical problems are solved. It describes the methods that research has shown to be most applicable in instruction to develop problem-solving abilities, and presents some educational principles that justify their application. The "trail-following" approach is the method that has been most commonly used to study the physician's problem-solving behavior. The salient conclusions from this research are that in the problem-solving process the diagnostic hypothesis is generated very early on and with limited data; the number of hypotheses is small; the problem-solving approach is specific to the type of medical problem and case in hand; and the accumulation of medical knowledge and experience forms the basis of clinical competence. Four methods for teaching the solution of problems are described: case presentation, the rain of ideas, the nominal groups technique and decision-making consensus, the census and analysis of forces in the field, and the analysis of clinical decisions. These methods are carried out in small groups. The advantages of the small groups are that the students are active participants in the learning process, they receive formative evaluation of their performance in a setting conductive to learning, and are able to interact with their instructor if he makes proper use of the right questioning techniques. While no single problem-solving method can be useful to all students or in all the problems they encounter

  4. Teaching effective problem solving skills to radiation protection students

    International Nuclear Information System (INIS)

    Waller, Edward

    2008-01-01

    Full text: Problem solving skills are essential for all radiation protection personnel. Although some students have more natural problem solving skills than others, all students require practice to become comfortable using these skills. At the University of Ontario Institute of Technology (UOIT), a unique one-semester course was developed as part of the core curriculum to teach students problem solving skills and elements of modelling and simulation. The underlying emphasis of the course was to allow students to develop their own problem solving strategies, both individually and in groups. Direction was provided on how to examine problems from different perspectives, and how to determine the proper root problem statement. A five-point problem solving strategy was presented as: 1) Problem definition; 2) Solution generation; 3) Decision; 4) Implementation; 5) Evaluation. Within the strategy, problem solving techniques were integrated from diverse areas such as: De Bono 's six thinking hats, Kepner-Tregoe decision analysis, Covey's seven habits of highly effective people, Reason's swiss cheese theory of complex failure, and Howlett's common failure modes. As part of the evaluation step, students critically explore areas such as ethics and environmental responsibility. In addition to exploring problem solving methods, students learn the usefulness of simulation methods, and how to model and simulate complex phenomena of relevance to radiation protection. Computational aspects of problem solving are explored using the commercially available MATLAB computer code. A number of case studies are presented as both examples and problems to the students. Emphasis was placed on solutions to problems of interest to radiation protection, health physics and nuclear engineering. A group project, pertaining to an accident or event related to the nuclear industry is a course requirement. Students learn to utilize common time and project management tools such as flowcharting, Pareto

  5. Reflection on problem solving in introductory and advanced physics

    Science.gov (United States)

    Mason, Andrew J.

    Reflection is essential in order to learn from problem solving. This thesis explores issues related to how reflective students are and how we can improve their capacity for reflection on problem solving. We investigate how students naturally reflect in their physics courses about problem solving and evaluate strategies that may teach them reflection as an integral component of problem-solving. Problem categorization based upon similarity of solution is a strategy to help them reflect about the deep features of the problems related to the physics principles involved. We find that there is a large overlap between the introductory and graduate students in their ability to categorize. Moreover, introductory students in the calculus-based courses performed better categorization than those in the algebra-based courses even though the categorization task is conceptual. Other investigations involved exploring if reflection could be taught as a skill on individual and group levels. Explicit self-diagnosis in recitation investigated how effectively students could diagnose their own errors on difficult problems, how much scaffolding was necessary for this purpose, and how effective transfer was to other problems employing similar principles. Difficulty in applying physical principles and difference between the self-diagnosed and transfer problems affected performance. We concluded that a sustained intervention is required to learn effective problem-solving strategies. Another study involving reflection on problem solving with peers suggests that those who reflected with peers drew more diagrams and had a larger gain from the midterm to final exam. Another study in quantum mechanics involved giving common problems in midterm and final exams and suggested that advanced students do not automatically reflect on their mistakes. Interviews revealed that even advanced students often focus mostly on exams rather than learning and building a robust knowledge structure. A survey was

  6. Peer instruction enhanced meaningful learning: ability to solve novel problems.

    Science.gov (United States)

    Cortright, Ronald N; Collins, Heidi L; DiCarlo, Stephen E

    2005-06-01

    Students must be able to interpret, relate, and incorporate new information with existing knowledge and apply the new information to solve novel problems. Peer instruction is a cooperative learning technique that promotes critical thinking, problem solving, and decision-making skills. Therefore, we tested the hypothesis that peer instruction enhances meaningful learning or transfer, defined as the student's ability to solve novel problems or the ability to extend what has been learned in one context to new contexts. To test this hypothesis, our undergraduate exercise physiology class of 38 students was randomly divided into two groups: group A (n = 19) and group B (n = 19). A randomized crossover design in which students either answered questions individually or during peer instruction was used to control for time and order effects. The first factor that influences meaningful learning is the degree of mastery of the original material. Importantly, peer instruction significantly enhanced mastery of the original material. Furthermore, the student's ability to solve novel problems was significantly enhanced following peer instruction. Thus pausing two to three times during a 50-min class to allow peer instruction enhanced the mastery of the original material and enhanced meaningful learning, i.e., the student's ability to solve novel problems.

  7. A Problem Solving Intervention for hospice caregivers: a pilot study.

    Science.gov (United States)

    Demiris, George; Oliver, Debra Parker; Washington, Karla; Fruehling, Lynne Thomas; Haggarty-Robbins, Donna; Doorenbos, Ardith; Wechkin, Hope; Berry, Donna

    2010-08-01

    The Problem Solving Intervention (PSI) is a structured, cognitive-behavioral intervention that provides people with problem-solving coping skills to help them face major negative life events and daily challenges. PSI has been applied to numerous settings but remains largely unexplored in the hospice setting. The aim of this pilot study was to demonstrate the feasibility of PSI targeting informal caregivers of hospice patients. We enrolled hospice caregivers who were receiving outpatient services from two hospice agencies. The intervention included three visits by a research team member. The agenda for each visit was informed by the problem-solving theoretical framework and was customized based on the most pressing problems identified by the caregivers. We enrolled 29 caregivers. Patient's pain was the most frequently identified problem. On average, caregivers reported a higher quality of life and lower level of anxiety postintervention than at baseline. An examination of the caregiver reaction assessment showed an increase of positive esteem average and a decrease of the average value of lack of family support, impact on finances, impact on schedules, and on health. After completing the intervention, caregivers reported lower levels of anxiety, improved problem solving skills, and a reduced negative impact of caregiving. Furthermore, caregivers reported high levels of satisfaction with the intervention, perceiving it as a platform to articulate their challenges and develop a plan to address them. Findings demonstrate the value of problem solving as a psycho-educational intervention in the hospice setting and call for further research in this area.

  8. Problem Solving Reasoning and Problem Based Instruction in Geometry Learning

    Science.gov (United States)

    Sulistyowati, F.; Budiyono, B.; Slamet, I.

    2017-09-01

    This research aims to analyze the comparison Problem Solving Reasoning (PSR) and Problem Based Instruction (PBI) on problem solving and mathematical communication abilities viewed from Self-Regulated Learning (SRL). Learning was given to grade 8th junior high school students. This research uses quasi experimental method, and then with descriptive analysis. Data were analyzed using two-ways multivariate analysis of variance (MANOVA) and one-way analysis of variance (ANOVA) with different cells. The result of data analysis were learning model gives different effect, level of SRL gives the same effect, and there is no interaction between the learning model with the SRL on the problem solving and mathematical communication abilities. The t-test statistic was used to find out more effective learning model. Based on the test, regardless of the level of SRL, PSR is more effective than PBI for problemsolving ability. The result of descriptive analysis was PSR had the advantage in creating learning that optimizing the ability of learners in reasoning to solve a mathematical problem. Consequently, the PSR is the right learning model to be applied in the classroom to improve problem solving ability of learners.

  9. Complex collaborative problem-solving processes in mission control.

    Science.gov (United States)

    Fiore, Stephen M; Wiltshire, Travis J; Oglesby, James M; O'Keefe, William S; Salas, Eduardo

    2014-04-01

    NASA's Mission Control Center (MCC) is responsible for control of the International Space Station (ISS), which includes responding to problems that obstruct the functioning of the ISS and that may pose a threat to the health and well-being of the flight crew. These problems are often complex, requiring individuals, teams, and multiteam systems, to work collaboratively. Research is warranted to examine individual and collaborative problem-solving processes in this context. Specifically, focus is placed on how Mission Control personnel-each with their own skills and responsibilities-exchange information to gain a shared understanding of the problem. The Macrocognition in Teams Model describes the processes that individuals and teams undertake in order to solve problems and may be applicable to Mission Control teams. Semistructured interviews centering on a recent complex problem were conducted with seven MCC professionals. In order to assess collaborative problem-solving processes in MCC with those predicted by the Macrocognition in Teams Model, a coding scheme was developed to analyze the interview transcriptions. Findings are supported with excerpts from participant transcriptions and suggest that team knowledge-building processes accounted for approximately 50% of all coded data and are essential for successful collaborative problem solving in mission control. Support for the internalized and externalized team knowledge was also found (19% and 20%, respectively). The Macrocognition in Teams Model was shown to be a useful depiction of collaborative problem solving in mission control and further research with this as a guiding framework is warranted.

  10. Problem solving verbal strategies in children with mild intellectual disability

    Directory of Open Access Journals (Sweden)

    Gligorović Milica

    2013-01-01

    Full Text Available Problem solving is a process conditioned by the development and application of efficient strategies. The aim of this research is to determine the level of verbal strategic approach to problem solving in children with mild intellectual disability (MID. The sample consists of 93 children with MID, aged between 10 and 14. Intellectual abilities of the examinees are within the defined range for mild intellectual disability (AM=60.45; SD=7.26. The examinees with evident physical, neurological, and emotional disorders were not included in the sample. The closed 20 Questions Test (20Q was used to assess the development and use of verbal strategy, where the examinee is presented with a poster containing 42 different pictures, and instructed to guess the picture selected by the examiner by asking no more than 20 closed questions. Test χ2, and Spearman and Pearson's correlation coefficient were used in statistical analysis. Research results indicate that most children with MID, aged between 10 and 14, use non-efficient strategy in solving the 20 Questions Test. Although strategic approach to problem solving is present in most children (72%, more than half of the examinees (53.5% use an inadequate strategy. Most children with MID have the ability to categorize concepts, however, they do not use it as a strategy in problem solving.

  11. Measures of Potential Flexibility and Practical Flexibility in Equation Solving

    Directory of Open Access Journals (Sweden)

    Le Xu

    2017-08-01

    Full Text Available Researchers interested in mathematical proficiency have recently begun to explore the development of strategic flexibility, where flexibility is defined as knowledge of multiple strategies for solving a problem and the ability to implement an innovative strategy for a given problem solving circumstance. However, anecdotal findings from this literature indicate that students do not consistently use an innovative strategy for solving a given problem, even when these same students demonstrate knowledge of innovative strategies. This distinction, sometimes framed in the psychological literature as competence vs. performance—has not been previously studied for flexibility. In order to explore the competence/performance distinction in flexibility, this study developed and validated measures for potential flexibility (e.g., competence, or knowledge of multiple strategies and practical flexibility (e.g., performance, use of innovative strategies for solving equations. The measures were administrated to a sample of 158 Chinese middle school students through a Tri-Phase Flexibility Assessment, in which the students were asked to solve each equation, generate additional strategies, and evaluate own multiple strategies. Confirmatory factor analysis supported a two-factor model of potential and practical flexibility. Satisfactory internal consistency was found for the measures. Additional validity evidence included the significant association with flexibility measured with the previous method. Potential flexibility and practical flexibility were found to be distinct but related. The theoretical and practical implications of the concepts and their measures of potential flexibility and practical flexibility are discussed.

  12. Neural activity when people solve verbal problems with insight.

    Directory of Open Access Journals (Sweden)

    Mark Jung-Beeman

    2004-04-01

    Full Text Available People sometimes solve problems with a unique process called insight, accompanied by an "Aha!" experience. It has long been unclear whether different cognitive and neural processes lead to insight versus noninsight solutions, or if solutions differ only in subsequent subjective feeling. Recent behavioral studies indicate distinct patterns of performance and suggest differential hemispheric involvement for insight and noninsight solutions. Subjects solved verbal problems, and after each correct solution indicated whether they solved with or without insight. We observed two objective neural correlates of insight. Functional magnetic resonance imaging (Experiment 1 revealed increased activity in the right hemisphere anterior superior temporal gyrus for insight relative to noninsight solutions. The same region was active during initial solving efforts. Scalp electroencephalogram recordings (Experiment 2 revealed a sudden burst of high-frequency (gamma-band neural activity in the same area beginning 0.3 s prior to insight solutions. This right anterior temporal area is associated with making connections across distantly related information during comprehension. Although all problem solving relies on a largely shared cortical network, the sudden flash of insight occurs when solvers engage distinct neural and cognitive processes that allow them to see connections that previously eluded them.

  13. Beyond Psychometrics: The Difference between Difficult Problem Solving and Complex Problem Solving

    Directory of Open Access Journals (Sweden)

    Jens F. Beckmann

    2017-10-01

    Full Text Available In this paper we argue that a synthesis of findings across the various sub-areas of research in complex problem solving and consequently progress in theory building is hampered by an insufficient differentiation of complexity and difficulty. In the proposed framework of person, task, and situation (PTS, complexity is conceptualized as a quality that is determined by the cognitive demands that the characteristics of the task and the situation impose. Difficulty represents the quantifiable level of a person’s success in dealing with such demands. We use the well-documented “semantic effect” as an exemplar for testing some of the conceptual assumptions derived from the PTS framework. We demonstrate how a differentiation between complexity and difficulty can help take beyond a potentially too narrowly defined psychometric perspective and subsequently gain a better understanding of the cognitive mechanisms behind this effect. In an empirical study a total of 240 university students were randomly allocated to one of four conditions. The four conditions resulted from contrasting the semanticity level of the variable labels used in the CPS system (high vs. low and two instruction conditions for how to explore the CPS system’s causal structure (starting with the assumption that all relationships between variables existed vs. starting with the assumption that none of the relationships existed. The variation in the instruction aimed at inducing knowledge acquisition processes of either (1 systematic elimination of presumptions, or (2 systematic compilation of a mental representation of the causal structure underpinning the system. Results indicate that (a it is more complex to adopt a “blank slate” perspective under high semanticity as it requires processes of inhibiting prior assumptions, and (b it seems more difficult to employ a systematic heuristic when testing against presumptions. In combination, situational characteristics, such as the

  14. The Future of Design: Unframed Problem Solving in Design Education

    DEFF Research Database (Denmark)

    Friis, Silje Alberthe Kamille; Gelting, Anne Katrine Gøtzsche

    2016-01-01

    The present paper sets out to investigate the impact and significance of a 3rd semester course in design methods, complex problem solving, and cross-disciplinary collaboration to the students within six design disciplines as experienced by the students three years later. The course reflects a shift...... in focus from a design practice framed by outcome to open-ended problem solving. Data shows that some students draw a direct line between the course and later success, for instance in obtaining an internship, whereas others find it of little use and relevance to later practice. Existing design research...... and theory is applied to further understand how to motivate design students to engage in an expanded field of design practice. Findings indicate that students need help to disengage from identifying with a particular design discipline if they are to engage in complex problem solving with an open mindset...

  15. Experimental quantum computing to solve systems of linear equations.

    Science.gov (United States)

    Cai, X-D; Weedbrook, C; Su, Z-E; Chen, M-C; Gu, Mile; Zhu, M-J; Li, Li; Liu, Nai-Le; Lu, Chao-Yang; Pan, Jian-Wei

    2013-06-07

    Solving linear systems of equations is ubiquitous in all areas of science and engineering. With rapidly growing data sets, such a task can be intractable for classical computers, as the best known classical algorithms require a time proportional to the number of variables N. A recently proposed quantum algorithm shows that quantum computers could solve linear systems in a time scale of order log(N), giving an exponential speedup over classical computers. Here we realize the simplest instance of this algorithm, solving 2×2 linear equations for various input vectors on a quantum computer. We use four quantum bits and four controlled logic gates to implement every subroutine required, demonstrating the working principle of this algorithm.

  16. EKSPERIMENTASI STRATEGI PEMBELAJARAN SYSTEMATIC PROBLEM SOLVING TERHADAP HASIL BELAJAR SISWA

    Directory of Open Access Journals (Sweden)

    Sulaiman Sulaiman

    2017-02-01

    Full Text Available This study aims to determine the difference in average math student learning outcomes obtained using systematic problem solving learning strategies with average math student learning outcomes obtained using expository teaching strategy. The population in this study is the first semester of class X  SMK Islam Adiluwih 2015-2016 school year, amounting to 137 people and is divided into five classes. The results of the data analysis test two parties at significant level 5% results obtained thitung = 3.66 and ttable = 1.99, which means there are differences in the average math student learning outcomes obtained using systematic learning strategy problem expository solving strategies. Then the t-test data analysis of the parties with a significant level of 5% obtained thitung = 3.66 and ttable = 1.67, which means the average student learning outcomes obtained using systematic problem solving learning strategy is better than expository teaching strategy.

  17. Students’ epistemological framing in quantum mechanics problem solving

    Directory of Open Access Journals (Sweden)

    Bahar Modir

    2017-08-01

    Full Text Available Students’ difficulties in quantum mechanics may be the result of unproductive framing and not a fundamental inability to solve the problems or misconceptions about physics content. We observed groups of students solving quantum mechanics problems in an upper-division physics course. Using the lens of epistemological framing, we investigated four frames in our observational data: algorithmic math, conceptual math, algorithmic physics, and conceptual physics. We discuss the characteristics of each frame as well as causes for transitions between different frames, arguing that productive problem solving may occur in any frame as long as students transition appropriately between frames. Our work extends epistemological framing theory on how students frame discussions in upper-division physics courses.

  18. Wavelet Methods for Solving Fractional Order Differential Equations

    Directory of Open Access Journals (Sweden)

    A. K. Gupta

    2014-01-01

    Full Text Available Fractional calculus is a field of applied mathematics which deals with derivatives and integrals of arbitrary orders. The fractional calculus has gained considerable importance during the past decades mainly due to its application in diverse fields of science and engineering such as viscoelasticity, diffusion of biological population, signal processing, electromagnetism, fluid mechanics, electrochemistry, and many more. In this paper, we review different wavelet methods for solving both linear and nonlinear fractional differential equations. Our goal is to analyze the selected wavelet methods and assess their accuracy and efficiency with regard to solving fractional differential equations. We discuss challenges faced by researchers in this field, and we emphasize the importance of interdisciplinary effort for advancing the study on various wavelets in order to solve differential equations of arbitrary order.

  19. Redesigning problem solving component in General Physics course.

    Science.gov (United States)

    Shakov, Jerry; McGuire, Jim

    2007-04-01

    Problem-based learning has been widely used in teaching introductory/general physics courses for a long time. The role of problem-solving sessions in the learning process is absolutely critical: they give the students an opportunity to learn how to apply both newly and previously acquired knowledge to practical situations, how to put together different strategies and portions of material, and much more. Unfortunately, the traditional format used for the problem solving sessions is not very accommodative for the goal: large class sizes and limited time often force instructors to spend most of the time solving sample problems in front of the class, which leaves the students with the role of passive observers. In this work, we will discuss how one can involve the students in the process of active learning using collaborative strategies and principles of cognitive apprenticeship.

  20. Teaching problem-solving skills to nuclear engineering students

    Science.gov (United States)

    Waller, E.; Kaye, M. H.

    2012-08-01

    Problem solving is an essential skill for nuclear engineering graduates entering the workforce. Training in qualitative and quantitative aspects of problem solving allows students to conceptualise and execute solutions to complex problems. Solutions to problems in high consequence fields of study such as nuclear engineering require rapid and accurate analysis of the problems, design of solutions (focusing on public safety, environmental stewardship and ethics), solution execution and monitoring results. A three-month course in problem solving, modelling and simulation was designed and a collaborative approach was undertaken with instructors from both industry and academia. Training was optimised for the laptop-based pedagogy, which provided unique advantages for a course that includes modelling and simulation components. The concepts and tools learned as part of the training were observed to be utilised throughout the duration of student university studies and interviews with students who have entered the workforce indicate that the approaches learned and practised are retained long term.

  1. Students' epistemological framing in quantum mechanics problem solving

    Science.gov (United States)

    Modir, Bahar; Thompson, John D.; Sayre, Eleanor C.

    2017-12-01

    Students' difficulties in quantum mechanics may be the result of unproductive framing and not a fundamental inability to solve the problems or misconceptions about physics content. We observed groups of students solving quantum mechanics problems in an upper-division physics course. Using the lens of epistemological framing, we investigated four frames in our observational data: algorithmic math, conceptual math, algorithmic physics, and conceptual physics. We discuss the characteristics of each frame as well as causes for transitions between different frames, arguing that productive problem solving may occur in any frame as long as students transition appropriately between frames. Our work extends epistemological framing theory on how students frame discussions in upper-division physics courses.

  2. Third International Conference on Soft Computing for Problem Solving

    CERN Document Server

    Deep, Kusum; Nagar, Atulya; Bansal, Jagdish

    2014-01-01

    The present book is based on the research papers presented in the 3rd International Conference on Soft Computing for Problem Solving (SocProS 2013), held as a part of the golden jubilee celebrations of the Saharanpur Campus of IIT Roorkee, at the Noida Campus of Indian Institute of Technology Roorkee, India. This book is divided into two volumes and covers a variety of topics including mathematical modelling, image processing, optimization, swarm intelligence, evolutionary algorithms, fuzzy logic, neural networks, forecasting, medical and health care, data mining etc. Particular emphasis is laid on soft computing and its application to diverse fields. The prime objective of the book is to familiarize the reader with the latest scientific developments that are taking place in various fields and the latest sophisticated problem solving tools that are being developed to deal with the complex and intricate problems, which are otherwise difficult to solve by the usual and traditional methods. The book is directed ...

  3. Solving Assembly Sequence Planning using Angle Modulated Simulated Kalman Filter

    Science.gov (United States)

    Mustapa, Ainizar; Yusof, Zulkifli Md.; Adam, Asrul; Muhammad, Badaruddin; Ibrahim, Zuwairie

    2018-03-01

    This paper presents an implementation of Simulated Kalman Filter (SKF) algorithm for optimizing an Assembly Sequence Planning (ASP) problem. The SKF search strategy contains three simple steps; predict-measure-estimate. The main objective of the ASP is to determine the sequence of component installation to shorten assembly time or save assembly costs. Initially, permutation sequence is generated to represent each agent. Each agent is then subjected to a precedence matrix constraint to produce feasible assembly sequence. Next, the Angle Modulated SKF (AMSKF) is proposed for solving ASP problem. The main idea of the angle modulated approach in solving combinatorial optimization problem is to use a function, g(x), to create a continuous signal. The performance of the proposed AMSKF is compared against previous works in solving ASP by applying BGSA, BPSO, and MSPSO. Using a case study of ASP, the results show that AMSKF outperformed all the algorithms in obtaining the best solution.

  4. Support method for solving an optimal xenon shutdown problem

    International Nuclear Information System (INIS)

    Dung, L.C.

    1992-01-01

    Since the discovering of the maximum principle by Pontriagin in 1956, methods for solving optimal control problems have been developed fast. There are the efforts to solve an optimal problem of transient process in a nuclear reactor using its ideas. However, the classical maximum principle does not show how to construct an optimal control or suboptimal control with a given exactness. We exploit mainly in the present work the ideas of the support method proposed by Gabasov and Kirillova for linear systems, in order to solve an optimal control problem for non-linear systems. The constructive maximum principle for non-linear dynamic systems with controllable structure received by us in this paper is new result. The ε - maximum principle is used for receiving an 7-phase ε - optimal control of optimal xenon shutdown problem. (author)

  5. Domain decomposition methods for solving an image problem

    Energy Technology Data Exchange (ETDEWEB)

    Tsui, W.K.; Tong, C.S. [Hong Kong Baptist College (Hong Kong)

    1994-12-31

    The domain decomposition method is a technique to break up a problem so that ensuing sub-problems can be solved on a parallel computer. In order to improve the convergence rate of the capacitance systems, pre-conditioned conjugate gradient methods are commonly used. In the last decade, most of the efficient preconditioners are based on elliptic partial differential equations which are particularly useful for solving elliptic partial differential equations. In this paper, the authors apply the so called covering preconditioner, which is based on the information of the operator under investigation. Therefore, it is good for various kinds of applications, specifically, they shall apply the preconditioned domain decomposition method for solving an image restoration problem. The image restoration problem is to extract an original image which has been degraded by a known convolution process and additive Gaussian noise.

  6. Second International Conference on Soft Computing for Problem Solving

    CERN Document Server

    Nagar, Atulya; Deep, Kusum; Pant, Millie; Bansal, Jagdish; Ray, Kanad; Gupta, Umesh

    2014-01-01

    The present book is based on the research papers presented in the International Conference on Soft Computing for Problem Solving (SocProS 2012), held at JK Lakshmipat University, Jaipur, India. This book provides the latest developments in the area of soft computing and covers a variety of topics, including mathematical modeling, image processing, optimization, swarm intelligence, evolutionary algorithms, fuzzy logic, neural networks, forecasting, data mining, etc. The objective of the book is to familiarize the reader with the latest scientific developments that are taking place in various fields and the latest sophisticated problem solving tools that are being developed to deal with the complex and intricate problems that are otherwise difficult to solve by the usual and traditional methods. The book is directed to the researchers and scientists engaged in various fields of Science and Technology.

  7. Information Seeking When Problem Solving: Perspectives of Public Health Professionals.

    Science.gov (United States)

    Newman, Kristine; Dobbins, Maureen; Yost, Jennifer; Ciliska, Donna

    2017-04-01

    Given the many different types of professionals working in public health and their diverse roles, it is likely that their information needs, information-seeking behaviors, and problem-solving abilities differ. Although public health professionals often work in interdisciplinary teams, few studies have explored their information needs and behaviors within the context of teamwork. This study explored the relationship between Canadian public health professionals' perceptions of their problem-solving abilities and their information-seeking behaviors with a specific focus on the use of evidence in practice settings. It also explored their perceptions of collaborative information seeking and the work contexts in which they sought information. Key Canadian contacts at public health organizations helped recruit study participants through their list-servs. An electronic survey was used to gather data about (a) individual information-seeking behaviors, (b) collaborative information-seeking behaviors, (c) use of evidence in practice environments, (d) perceived problem-solving abilities, and (e) demographic characteristics. Fifty-eight public health professionals were recruited, with different roles and representing most Canadian provinces and one territory. A significant relationship was found between perceived problem-solving abilities and collaborative information-seeking behavior (r = -.44, p information seeking. The results suggested that when public health professionals take a shared, active approach to problem solving, maintain personal control, and have confidence, they are more likely collaborate with others in seeking information to complete a work task. Administrators of public health organizations should promote collaboration by implementing effective communication and information-seeking strategies, and by providing information resources and retrieval tools. Public health professionals' perceived problem-solving abilities can influence how they collaborate in

  8. Solving Systems of Equations with Techniques from Artificial Intelligence

    Directory of Open Access Journals (Sweden)

    Irina Maria Terfaloaga

    2015-07-01

    Full Text Available A frequent problem in numerical analysis is solving the systems of equations. That problem has generated in time a great interest among mathematicians and computer scientists, as evidenced by the large number of numerical methods developed. Besides the classical numerical methods, in the last years were proposed methods inspired by techniques from artificial intelligence. Hybrid methods have been also proposed along the time [15, 19]. The goal of this study is to make a survey of methods inspired from artificial intelligence for solving systems of equations

  9. Solving Large Clustering Problems with Meta-Heuristic Search

    DEFF Research Database (Denmark)

    Turkensteen, Marcel; Andersen, Kim Allan; Bang-Jensen, Jørgen

    problems, but applications to large clustering problems are rare. The simulated annealing heuristic has mainly been applied to relatively small instances. In this paper, we implement tabu search and simulated annealing approaches and compare them to the commonly used k-means approach. We find that the meta......In Clustering Problems, groups of similar subjects are to be retrieved from data sets. In this paper, Clustering Problems with the frequently used Minimum Sum-of-Squares Criterion are solved using meta-heuristic search. Tabu search has proved to be a successful methodology for solving optimization...

  10. Solving PDEs in Python the FEniCS tutorial I

    CERN Document Server

    Langtangen, Hans Petter

    2016-01-01

    This book offers a concise and gentle introduction to finite element programming in Python based on the popular FEniCS software library. Using a series of examples, including the Poisson equation, the equations of linear elasticity, the incompressible Navier–Stokes equations, and systems of nonlinear advection–diffusion–reaction equations, it guides readers through the essential steps to quickly solving a PDE in FEniCS, such as how to define a finite variational problem, how to set boundary conditions, how to solve linear and nonlinear systems, and how to visualize solutions and structure finite element Python programs. This book is open access under a CC BY license.

  11. An efficient numerical method for solving nonlinear foam drainage equation

    Science.gov (United States)

    Parand, Kourosh; Delkhosh, Mehdi

    2018-02-01

    In this paper, the nonlinear foam drainage equation, which is a famous nonlinear partial differential equation, is solved by using a hybrid numerical method based on the quasilinearization method and the bivariate generalized fractional order of the Chebyshev functions (B-GFCF) collocation method. First, using the quasilinearization method, the equation is converted into a sequence of linear partial differential equations (LPD), and then these LPDs are solved using the B-GFCF collocation method. A very good approximation of solutions is obtained, and comparisons show that the obtained results are more accurate than the results of other researchers.

  12. Solving the Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Askelsdottir, Björg; Jensen, Rune Møller

    2015-01-01

    We solve a central problem in the liner shipping industry called the liner shipping fleet repositioning problem (LSFRP). The LSFRP poses a large financial burden on liner shipping firms. During repositioning, vessels are moved between routes in a liner shipping network. Liner carriers wish...... introduce a novel mathematical model and a simulated annealing algorithm for the LSFRP with cargo flows that makes use of a carefully constructed graph; we evaluate these approaches using real-world data from our industrial collaborator. Additionally, we compare the performance of our approach against...... to solve the LSFRP....

  13. On the Applicability of Lower Bounds for Solving Rectilinear

    DEFF Research Database (Denmark)

    Clausen, Jens; Karisch, Stefan E.; Perregaard, M.

    1998-01-01

    The quadratic assignment problem (QAP) belongs to the hard core of NP-hard optimization problems. After almost forty years of research only relatively small instances can be solved to optimality. The reason is that the quality of the lower bounds available for exact methods is not sufficient....... Recently, lower bounds based on decomposition were proposed for the so called rectilinear QAP that proved to be the strongest for a large class of problem instances. We investigate the strength of these bounds when applied not only at the root node of a search tree but as the bound function used...... in a Branch-and-Bound code solving large scale QAPs....

  14. Exact Methods for Solving the Train Departure Matching Problem

    DEFF Research Database (Denmark)

    Haahr, Jørgen Thorlund; Bull, Simon Henry

    In this paper we consider the train departure matching problem which is an important subproblem of the Rolling Stock Unit Management on Railway Sites problem introduced in the ROADEF/EURO Challenge 2014. The subproblem entails matching arriving train units to scheduled departing trains at a railway...... site while respecting multiple physical and operational constraints. In this paper we formally define that subproblem, prove its NP- hardness, and present two exact method approaches for solving the problem. First, we present a compact Mixed Integer Program formulation which we solve using a MIP solver...

  15. Doing physics with scientific notebook a problem solving approach

    CERN Document Server

    Gallant, Joseph

    2012-01-01

    The goal of this book is to teach undergraduate students how to use Scientific Notebook (SNB) to solve physics problems. SNB software combines word processing and mathematics in standard notation with the power of symbolic computation. As its name implies, SNB can be used as a notebook in which students set up a math or science problem, write and solve equations, and analyze and discuss their results. Written by a physics teacher with over 20 years experience, this text includes topics that have educational value, fit within the typical physics curriculum, and show the benefits of using SNB.

  16. Multiparameter extrapolation and deflation methods for solving equation systems

    Directory of Open Access Journals (Sweden)

    A. J. Hughes Hallett

    1984-01-01

    Full Text Available Most models in economics and the applied sciences are solved by first order iterative techniques, usually those based on the Gauss-Seidel algorithm. This paper examines the convergence of multiparameter extrapolations (accelerations of first order iterations, as an improved approximation to the Newton method for solving arbitrary nonlinear equation systems. It generalises my earlier results on single parameter extrapolations. Richardson's generalised method and the deflation method for detecting successive solutions in nonlinear equation systems are also presented as multiparameter extrapolations of first order iterations. New convergence results are obtained for those methods.

  17. Solving traveling salesman problems with DNA molecules encoding numerical values.

    Science.gov (United States)

    Lee, Ji Youn; Shin, Soo-Yong; Park, Tai Hyun; Zhang, Byoung-Tak

    2004-12-01

    We introduce a DNA encoding method to represent numerical values and a biased molecular algorithm based on the thermodynamic properties of DNA. DNA strands are designed to encode real values by variation of their melting temperatures. The thermodynamic properties of DNA are used for effective local search of optimal solutions using biochemical techniques, such as denaturation temperature gradient polymerase chain reaction and temperature gradient gel electrophoresis. The proposed method was successfully applied to the traveling salesman problem, an instance of optimization problems on weighted graphs. This work extends the capability of DNA computing to solving numerical optimization problems, which is contrasted with other DNA computing methods focusing on logical problem solving.

  18. Problem Solving in Physics: Undergraduates' Framing, Procedures, and Decision Making

    Science.gov (United States)

    Modir, Bahar

    In this dissertation I will start with the broad research question of what does problem solving in upper division physics look like? My focus in this study is on students' problem solving in physics theory courses. Some mathematical formalisms are common across all physics core courses such as using the process of separation of variables, doing Taylor series, or using the orthogonality properties of mathematical functions to set terms equal to zero. However, there are slight differences in their use of these mathematical formalisms across different courses, possibly because of how students map different physical systems to these processes. Thus, my first main research question aims to answer how students perform these recurring processes across upper division physics courses. I break this broad question into three particular research questions: What knowledge pieces do students use to make connections between physics and procedural math? How do students use their knowledge pieces coherently to provide reasoning strategies in estimation problems? How do students look ahead into the problem to read the information out of the physical scenario to align their use of math in physics? Building on the previous body of the literature, I will use the theory family of Knowledge in Pieces and provide evidence to expand this theoretical foundation. I will compare my study with previous studies and provide suggestions on how to generalize these theory expansions for future use. My experimental data mostly come from video-based classroom data. Students in groups of 2-4 students solve in-class problems in quantum mechanics and electromagnetic fields 1 courses collaboratively. In addition, I will analyze clinical interviews to demonstrate how a single case study student plays an epistemic game to estimate the total energy in a hurricane. My second research question is more focused on a particular instructional context. How do students frame problem solving in quantum mechanics? I

  19. Applying Groebner bases to solve reduction problems for Feynman integrals

    International Nuclear Information System (INIS)

    Smirnov, Alexander V.; Smirnov, Vladimir A.

    2006-01-01

    We describe how Groebner bases can be used to solve the reduction problem for Feynman integrals, i.e. to construct an algorithm that provides the possibility to express a Feynman integral of a given family as a linear combination of some master integrals. Our approach is based on a generalized Buchberger algorithm for constructing Groebner-type bases associated with polynomials of shift operators. We illustrate it through various examples of reduction problems for families of one- and two-loop Feynman integrals. We also solve the reduction problem for a family of integrals contributing to the three-loop static quark potential

  20. A Predictor-Corrector Method for Solving Equilibrium Problems

    Directory of Open Access Journals (Sweden)

    Zong-Ke Bao

    2014-01-01

    Full Text Available We suggest and analyze a predictor-corrector method for solving nonsmooth convex equilibrium problems based on the auxiliary problem principle. In the main algorithm each stage of computation requires two proximal steps. One step serves to predict the next point; the other helps to correct the new prediction. At the same time, we present convergence analysis under perfect foresight and imperfect one. In particular, we introduce a stopping criterion which gives rise to Δ-stationary points. Moreover, we apply this algorithm for solving the particular case: variational inequalities.

  1. The Teacher as a Role Model in Problem Solving

    Science.gov (United States)

    Cherkas, Barry M.

    1978-01-01

    The author discusses the use of free-hand drawing, the modified Socratic teaching method, and the differentiation of mathematical language from colloquial usage as techniques which he has found helpful in creating a positive classroom environment for teaching problem solving. (MN)

  2. Application of the trial equation method for solving some nonlinear ...

    Indian Academy of Sciences (India)

    Their key idea is to expand solutions of given differential equations as functions of solutions of solvable differential equations, in particular, polynomial and rational func- tions. This idea is so good that many types of nonlinear differential equations can be solved by it. Also, Liu proposed the trial equation method and applied ...

  3. He's iteration formulation for solving nonlinear algebraic equations

    International Nuclear Information System (INIS)

    Qian, W-X; Ye, Y-H; Chen, J; Mo, L-F

    2008-01-01

    Newton iteration method is sensitive to initial guess and its slope. To overcome the shortcoming, He's iteration method is used to solve nonlinear algebraic equations where Newton iteration method becomes invalid. Some examples are given, showing that the method is effective

  4. A highly accurate method to solve Fisher's equation

    Indian Academy of Sciences (India)

    ) for our discussion, one can apply the proposed method to solve the more general problem. ∂u. ∂t. = ∂2u. ∂x2 + F(u), where F is a real function. Therefore, we consider this problem for our numerical examples. Example 1. Consider the ...

  5. Problem-Solving Communication in Foster Families and Birthfamilies.

    Science.gov (United States)

    Vuchinich, Sam; Ozretich, Rachel A.; Pratt, Clara C.; Kneedler, Blythe

    2002-01-01

    Assessed child behavior problems and parent-child communication behaviors during problem solving in three groups of families with adolescents: foster families, birthfamilies with a child at risk for behavior problems, and birthfamilies with a child not at risk. Found that levels of positive and negative communication behaviors in foster families…

  6. Solving Fractional Programming Problems based on Swarm Intelligence

    Science.gov (United States)

    Raouf, Osama Abdel; Hezam, Ibrahim M.

    2014-04-01

    This paper presents a new approach to solve Fractional Programming Problems (FPPs) based on two different Swarm Intelligence (SI) algorithms. The two algorithms are: Particle Swarm Optimization, and Firefly Algorithm. The two algorithms are tested using several FPP benchmark examples and two selected industrial applications. The test aims to prove the capability of the SI algorithms to solve any type of FPPs. The solution results employing the SI algorithms are compared with a number of exact and metaheuristic solution methods used for handling FPPs. Swarm Intelligence can be denoted as an effective technique for solving linear or nonlinear, non-differentiable fractional objective functions. Problems with an optimal solution at a finite point and an unbounded constraint set, can be solved using the proposed approach. Numerical examples are given to show the feasibility, effectiveness, and robustness of the proposed algorithm. The results obtained using the two SI algorithms revealed the superiority of the proposed technique among others in computational time. A better accuracy was remarkably observed in the solution results of the industrial application problems.

  7. Writing and mathematical problem solving in Grade 3

    Directory of Open Access Journals (Sweden)

    Belinda Petersen

    2017-06-01

    Full Text Available This article looks at writing tasks as a methodology to support learners’ mathematical problemsolving strategies in the South African Foundation Phase context. It is a qualitative case study and explores the relation between the use of writing in mathematics and development of learners’ problem-solving strategies and conceptual understanding. The research was conducted in a suburban Foundation Phase school in Cape Town with a class of Grade 3 learners involved in a writing and mathematics intervention. Writing tasks were modelled to learners and implemented by them while they were engaged in mathematical problem solving. Data were gathered from a sample of eight learners of different abilities and included written work, interviews, field notes and audio recordings of ability group discussions. The results revealed an improvement in the strategies and explanations learners used when solving mathematical problems compared to before the writing tasks were implemented. Learners were able to reflect critically on their thinking through their written strategies and explanations. The writing tasks appeared to support learners in providing opportunities to construct and apply mathematical knowledge and skills in their development of problem-solving strategies.

  8. Solving Optimization Problems with Dynamic Geometry Software: The Airport Problem

    Science.gov (United States)

    Contreras, José

    2014-01-01

    This paper describes how the author's students (in-service and pre-service secondary mathematics teachers) enrolled in college geometry courses use the Geometers' Sketchpad (GSP) to gain insight to formulate, confirm, test, and refine conjectures to solve the classical airport problem for triangles. The students are then provided with strategic…

  9. Designing WebQuests to Support Creative Problem Solving

    Science.gov (United States)

    Rubin, Jim

    2013-01-01

    WebQuests have been a popular alternative for collaborative group work that utilizes internet resources, but studies have questioned how effective they are in challenging students to use higher order thinking processes that involve creative problem solving. This article explains how different levels of inquiry relate to categories of learning…

  10. Solving Minimum Cost Multi-Commodity Network Flow Problem ...

    African Journals Online (AJOL)

    ADOWIE PERE

    the linear function of the ith goal; bi - an aspiration level of the ith goal; X - the vector of decision variables; F - the feasible set of constraints. Lexicographic Goal Programming was used in this work being an approach that solves Goal. Programming problems with priorities on objectives in order achieve travel time ...

  11. Bellman – Ford Method for Solving the Optimal Route Problem

    Directory of Open Access Journals (Sweden)

    Laima Greičiūnė

    2014-12-01

    Full Text Available The article aims to adapt the dynamic programming method for optimal route determination using real-time data on ITS equipment. For this purpose, VBA code has been applied for solving the Bellman - Ford method for an optimal route considering optimality criteria for time, distance and the amount of emissions.

  12. Solution Tree Problem Solving Procedure for Engineering Analysis ...

    African Journals Online (AJOL)

    This paper presents a 10-step procedure, called the solution-tree problemsolving procedure, for solving engineering analysis problems. The core of the procedure is the development of a tree-like solution algorithm for the problem or class of problems, based on the divide-and-conquer and top-down design concepts.

  13. Schoenfeld's problem solving theory in a student controlled learning environment

    NARCIS (Netherlands)

    Harskamp, E.; Suhre, C.

    2007-01-01

    This paper evaluates the effectiveness of a student controlled computer program for high school mathematics based on instruction principles derived from Schoenfeld's theory of problem solving. The computer program allows students to choose problems and to make use of hints during different episodes

  14. A numerical method for solving singular De`s

    Energy Technology Data Exchange (ETDEWEB)

    Mahaver, W.T.

    1996-12-31

    A numerical method is developed for solving singular differential equations using steepest descent based on weighted Sobolev gradients. The method is demonstrated on a variety of first and second order problems, including linear constrained, unconstrained, and partially constrained first order problems, a nonlinear first order problem with irregular singularity, and two second order variational problems.

  15. George Pólya & Problem Solving ... An Appreciation

    Indian Academy of Sciences (India)

    IAS Admin

    P¶olya's work in problem-solving dates to the time when he and fellow Hungarian G¶abor SzegÄo collaborated to ... in two volumes, subtitled `Induction and Analogy in. Mathematics' and `Patterns of Plausible Inference'; and .... Here, the sheer visual complexity is off-putting. What would be P¶olya's approach now? It seems ...

  16. Solving the TTC 2013 Flowgraphs Case with FunnyQT

    Directory of Open Access Journals (Sweden)

    Tassilo Horn

    2013-11-01

    Full Text Available FunnyQT is a model querying and model transformation library for the functional Lisp-dialect Clojure providing a rich and efficient querying and transformation API. This paper describes the FunnyQT solution to the TTC 2013 Flowgraphs Transformation Case. It solves all four tasks, and it has won the best efficiency award for this case.

  17. Young doctors' problem solving strategies on call may be improved

    DEFF Research Database (Denmark)

    Michelsen, Jens; Malchow-Møller, Axel; Charles, Peder

    2013-01-01

    The first year following graduation from medical school is challenging as learning from books changes to workplace-based learning. Analysis and reflection on experience may ease this transition. We used Significant Event Analysis (SEA) as a tool to explore what pre-registration house officers (PR...... (PRHOs) consider successful and problematic events, and to identify what problem-solving strategies they employ....

  18. Students' Competence in some Problem Solving Skills throughout ...

    African Journals Online (AJOL)

    The main objective of the study was to test students' competence, throughout all their years (first, second and final years) of a B.Sc. course, in five important types of problem solving skills: information processing skills; skills concerning equations; graphical skills; three-dimensional visualization skills and inverse proportion ...

  19. Diachrony in Human Cognition and Problem Solving

    DEFF Research Database (Denmark)

    Gahrn-Andersen, Rasmus

    2017-01-01

    Problem solving should not be reduced to situated or localized activity since cognizers also draw on non-local resources that are not actually experienced but which nevertheless impart on their situated cognition. A Varelianinspired epistemology neglects this nonlocality, which is a vital trait...

  20. Statistical evaluation of mathematical methods in solving linear ...

    African Journals Online (AJOL)

    Statistical evaluation of mathematical methods in solving linear theory problems: Design of water distribution systems. ... The flow obtained using the methods were evaluated using model of selection criterion MSC, coefficient of determination CD, reliability RD and errors. The study revealed that flow in pipe network ...