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Sample records for thermobifida fusca cel9a-90

  1. Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca

    NARCIS (Netherlands)

    Gaber, Yasser; Mekasha, Sophanit; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H; Fraaije, Marco W

    Thermobifida fusca is a well-known cellulose-degrading actinomycete, which produces various glycoside hydrolases for this purpose. However, despite the presence of putative chitinase genes in its genome, T. fusca has not been reported to grow on chitin as sole carbon source. In this study, a gene

  2. Not so monofunctional-a case of thermostable Thermobifida fusca catalase with peroxidase activity

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    Lončar, Nikola; Fraaije, Marco W

    Thermobifida fusca is a mesothermophilic organism known for its ability to degrade plant biomass and other organics, and it was demonstrated that it represents a rich resource of genes encoding for potent enzymes for biocatalysis. The thermostable catalase from T. fusca has been cloned and

  3. Genome Sequence and Analysis of the Soil Cellulolytic ActinomyceteThermobifida fusca

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    Lykidis, Athanasios; Mavromatis, Konstantinos; Ivanova, Natalia; Anderson, Iain; Land, Miriam; DiBartolo, Genevieve; Martinez, Michele; Lapidus, Alla; Lucas, Susan; Copeland, Alex; Richardson, Paul; Wilson,David B.; Kyrpides, Nikos

    2007-02-01

    Thermobifida fusca is a moderately thermophilic soilbacterium that belongs to Actinobacteria. 3 It is a major degrader ofplant cell walls and has been used as a model organism for the study of 4secreted, thermostable cellulases. The complete genome sequence showedthat T. fusca has a 5 single circular chromosome of 3642249 bp predictedto encode 3117 proteins and 65 RNA6 species with a coding densityof 85percent. Genome analysis revealed the existence of 29 putative 7glycoside hydrolases in addition to the previously identified cellulasesand xylanases. The 8 glycosyl hydrolases include enzymes predicted toexhibit mainly dextran/starch and xylan 9 degrading functions. T. fuscapossesses two protein secretion systems: the sec general secretion 10system and the twin-arginine translocation system. Several of thesecreted cellulases have 11 sequence signatures indicating theirsecretion may be mediated by the twin-arginine12 translocation system. T.fusca has extensive transport systems for import of carbohydrates 13coupled to transcriptional regulators controlling the expression of thetransporters and14 glycosylhydrolases. In addition to providing anoverview of the physiology of a soil 15 actinomycete, this study presentsinsights on the transcriptional regulation and secretion of16 cellulaseswhich may facilitate the industrial exploitation of thesesystems.

  4. Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca.

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    Gaber, Yasser; Mekasha, Sophanit; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H; Fraaije, Marco W

    2016-09-01

    Thermobifida fusca is a well-known cellulose-degrading actinomycete, which produces various glycoside hydrolases for this purpose. However, despite the presence of putative chitinase genes in its genome, T. fusca has not been reported to grow on chitin as sole carbon source. In this study, a gene encoding a putative membrane-anchored GH18 chitinase (Tfu0868) from T. fusca has been cloned and overexpressed in Escherichia coli. The protein was produced as SUMO fusion protein and, upon removal of the SUMO domain, soluble pure TfChi18A was obtained with yields typically amounting to 150mg per litre of culture. The enzyme was found to be relatively thermostable (apparent Tm=57.5°C) but not particularly thermoactive, the optimum temperature being 40-45°C. TfChi18A bound to α- and β-chitin and degraded both these substrates. Interestingly, activity towards colloidal chitin was minimal and in this case, substrate inhibition was observed. TfChi18A also cleaved soluble chito-oligosaccharides and showed a clear preference for substrates having five sugars or more. While these results show that TfChi18A is a catalytically competent GH18 chitinase, the observed catalytic rates were low compared to those of well-studied GH18 chitinases. This suggests that TfChi18A is not a true chitinase and not likely to endow T. fusca with the ability to grow on chitin. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca

    NARCIS (Netherlands)

    Schallmey, Anett; den Besten, Gijs; Teune, Ite G. P.; Kembaren, Roga F.; Janssen, Dick B.

    Cytochrome P450 monooxygenases are valuable biocatalysts due to their ability to hydroxylate unactivated carbon atoms using molecular oxygen. We have cloned the gene for a new cytochrome P450 monooxygenase, named CYP154H1, from the moderately thermophilic soil bacterium Thermobifida fusca. The

  6. Not so monofunctional--a case of thermostable Thermobifida fusca catalase with peroxidase activity.

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    Lončar, Nikola; Fraaije, Marco W

    2015-03-01

    Thermobifida fusca is a mesothermophilic organism known for its ability to degrade plant biomass and other organics, and it was demonstrated that it represents a rich resource of genes encoding for potent enzymes for biocatalysis. The thermostable catalase from T. fusca has been cloned and overexpressed in Escherichia coli with a yield of 400 mg/L. Heat treatment of disrupted cells at 60 °C for 1 h resulted in enzyme preparation of high purity; hence, no chromatography steps are needed for large-scale production. Except for catalyzing the dismutation of hydrogen peroxide, TfuCat was also found to catalyze oxidations of phenolic compounds. The catalase activity was comparable to other described catalases while peroxidase activity was quite remarkable with a k obs of nearly 1000 s(-1) for catechol. Site directed mutagenesis was used to alter the ratio of peroxidase/catalase activity. Resistance to inhibition by classic catalase inhibitors and an apparent melting temperature of 74 °C classifies this enzyme as a robust biocatalyst. As such, it could compete with other commercially available catalases while the relatively high peroxidase activity also offers new biocatalytic possibilities.

  7. Computational engineering of cellulase Cel9A-68 functional motions through mutations in its linker region.

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    Costa, M G S; Silva, Y F; Batista, P R

    2018-03-14

    Microbial cellulosic degradation by cellulases has become a complementary approach for biofuel production. However, its efficiency is hindered by the recalcitrance of cellulose fibres. In this context, computational protein design methods may offer an efficient way to obtain variants with improved enzymatic activity. Cel9A-68 is a cellulase from Thermobifida fusca that is still active at high temperatures. In a previous work, we described a collective bending motion, which governs the overall cellulase dynamics. This movement promotes the approximation of its CBM and CD structural domains (that are connected by a flexible linker). We have identified two residues (G460 and P461) located at the linker that act as a hinge point. Herein, we applied a new level of protein design, focusing on the modulation of this collective motion to obtain cellulase variants with enhanced functional dynamics. We probed whether specific linker mutations would affect Cel9A-68 dynamics through computational simulations. We assumed that P461G and G460+ (with an extra glycine) constructs would present enhanced interdomain motions, while the G460P mutant would be rigid. From our results, the P461G mutation resulted in a broader exploration of the conformational space, as confirmed by clustering and free energy analyses. The WT enzyme was the most rigid system. However, G460P and P460+ explored distinct conformational states described by opposite directions of low-frequency normal modes; they sampled preferentially closed and open conformations, respectively. Overall, we highlight two significant findings: (i) all mutants explored larger conformational spaces than the WT; (ii) the selection of distinct conformational populations was intimately associated with the mutation considered. Thus, the engineering of Cel9A-68 motions through linker mutations may constitute an efficient way to improve cellulase activity, facilitating the disruption of cellulose fibres.

  8. A New Esterase from Thermobifida halotolerans Hydrolyses Polyethylene Terephthalate (PET and Polylactic Acid (PLA

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    Georg Steinkellner

    2012-02-01

    Full Text Available A new esterase from Thermobifida halotolerans (Thh_Est was cloned and expressed in E. coli and investigated for surface hydrolysis of polylactic acid (PLA and polyethylene terephthalate (PET. Thh_Est is a member of the serine hydrolases superfamily containing the -GxSxG- motif with 85–87% homology to an esterase from T. alba, to an acetylxylan esterase from T. fusca and to various Thermobifida cutinases. Thh_Est hydrolyzed the PET model substrate bis(benzoyloxyethylterephthalate and PET releasing terephthalic acid and mono-(2-hydroxyethyl terephthalate in comparable amounts (19.8 and 21.5 mmol/mol of enzyme while no higher oligomers like bis-(2-hydroxyethyl terephthalate were detected. Similarly, PLA was hydrolyzed as indicated by the release of lactic acid. Enzymatic surface hydrolysis of PET and PLA led to a strong hydrophilicity increase, as quantified with a WCA decrease from 90.8° and 75.5° to 50.4° and to a complete spread of the water drop on the surface, respectively.

  9. Structure of Thermobifida fusca DyP-type peroxidase and activity towards Kraft lignin and lignin model compounds.

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    Rahmanpour, Rahman; Rea, Dean; Jamshidi, Shirin; Fülöp, Vilmos; Bugg, Timothy D H

    2016-03-15

    A Dyp-type peroxidase enzyme from thermophilic cellulose degrader Thermobifida fusca (TfuDyP) was investigated for catalytic ability towards lignin oxidation. TfuDyP was characterised kinetically against a range of phenolic substrates, and a compound I reaction intermediate was observed via pre-steady state kinetic analysis at λmax 404 nm. TfuDyP showed reactivity towards Kraft lignin, and was found to oxidise a β-aryl ether lignin model compound, forming an oxidised dimer. A crystal structure of TfuDyP was determined, to 1.8 Å resolution, which was found to contain a diatomic oxygen ligand bound to the heme centre, positioned close to active site residues Asp-203 and Arg-315. The structure contains two channels providing access to the heme cofactor for organic substrates and hydrogen peroxide. Site-directed mutant D203A showed no activity towards phenolic substrates, but reduced activity towards ABTS, while mutant R315Q showed no activity towards phenolic substrates, nor ABTS. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3

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    Su Lingqia

    2012-01-01

    Full Text Available Abstract Background Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory system in Escherichia coli BL21(DE3, and it was found that parts of the expressed protein was accumulated in the periplasmic space. Due to the fact that alpha-hemolysin secretion system can export target proteins directly from cytoplasm across both cell membrane of E. coli to the culture medium, thus in the present study we investigated the expression of cutinase using this alpha-hemolysin secretion system. Results T. fusca cutinase was fused with the specific signal peptide of alpha-hemolysin scretion system and expressed in E. coli BL21(DE3. In addition, HlyB and HlyD, strain-specific translocation components of alpha-hemolysin secretion system, were coexpressed to facilitate the enzyme expression. The cultivation of this engineered cell showed that cutinase activity in the culture medium reached 334 U/ml, which is 2.5 times that from type II secretion pathway under the same culture condition. The recombinant cutinase was further purified. Biochemical characterization of purified enzyme, which had an α-hemolysin secretion pathway signal peptide attached, had substrate specificity, pH and temperature profile, as well as application capability in bioscouring similar to that of wild-type cutinase. Conclusions In the present study, T. fusca cutinase was successfully secreted to the culture media by α-hemolysin secretion system. This is the first report of cutinase being efficiently secreted by this pathway. Due to the limited cases of successful expression of industrial enzyme by E. coli α-hemolysin secretion system, our study further explored the utilization of this pathway in industrial enzymes.

  11. Cloning, Expression and Biochemical Characterization of Endomannanases from Thermobifida Species Isolated from Different Niches.

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    Ákos Tóth

    Full Text Available Thermobifidas are thermotolerant, compost inhabiting actinomycetes which have complex polysaccharide hydrolyzing enzyme systems. The best characterized enzymes of these hydrolases are cellulases from T. fusca, while other important enzymes especially hemicellulases are not deeply explored. To fill this gap we cloned and investigated endomannanases from those reference strains of the Thermobifida genus, which have published data on other hydrolases (T. fusca TM51, T. alba CECT3323, T. cellulosilytica TB100T and T. halotolerans YIM90462T. Our phylogenetic analyses of 16S rDNA and endomannanase sequences revealed that T. alba CECT3323 is miss-classified; it belongs to the T. fusca species. The cloned and investigated endomannanases belong to the family of glycosyl hydrolases 5 (GH5, their size is around 50 kDa and they are modular enzymes. Their catalytic domains are extended by a C-terminal carbohydrate binding module (CBM of type 2 with a 23-25 residues long interdomain linker region consisting of Pro, Thr and Glu/Asp rich repetitive tetrapeptide motifs. Their polypeptide chains exhibit high homology, interdomain sequence, which don't show homology to each other, but all of them are built up from 3-6 times repeated tetrapeptide motifs (PTDP-Tc, TEEP-Tf, DPGT-Th. All of the heterologously expressed Man5A enzymes exhibited activity only on mannan. The pH optima of Man5A enzymes from T. halotolerans, T. cellulosilytica and T. fusca are slightly different (7.0, 7.5 and 8.0, respectively while their temperature optima span within the range of 70-75°C. The three endomannanases exhibited very similar kinetic performances on LBG-mannan substrate: 0.9-1.7mM of KM and 80-120 1/sec of turnover number. We detected great variability in heat stability at 70°C, which was influenced by the presence of Ca2+. The investigated endomannanases might be important subjects for studying the structure/function relation behind the heat stability and for industrial

  12. Cellulase variants

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    Blazej, Robert; Toriello, Nicholas; Emrich, Charles; Cohen, Richard N.; Koppel, Nitzan

    2015-07-14

    This invention provides novel variant cellulolytic enzymes having improved activity and/or stability. In certain embodiments the variant cellulotyic enzymes comprise a glycoside hydrolase with or comprising a substitution at one or more positions corresponding to one or more of residues F64, A226, and/or E246 in Thermobifida fusca Cel9A enzyme. In certain embodiments the glycoside hydrolase is a variant of a family 9 glycoside hydrolase. In certain embodiments the glycoside hydrolase is a variant of a theme B family 9 glycoside hydrolase.

  13. Bridging Theory and Experiment to Address Structural Properties of Truncated Haemoglobins: Insights from Thermobifida fusca HbO.

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    Howes, Barry D; Boechi, Leonardo; Boffi, Alberto; Estrin, Dario E; Smulevich, Giulietta

    2015-01-01

    In this chapter, we will discuss the paradigmatic case of Thermobifida fusca (Tf-trHb) HbO in its ferrous and ferric states and its behaviour towards a battery of possible ligands. This choice was dictated by the fact that it has been one of the most extensively studied truncated haemoglobins, both in terms of spectroscopic and molecular dynamics studies. Tf-trHb typifies the structural properties of group II trHbs, as the active site is characterized by a highly polar distal environment in which TrpG8, TyrCD1, and TyrB10 provide three potential H-bond donors in the distal cavity capable of stabilizing the incoming ligands. The role of these residues in key topological positions, and their interplay with the iron-bound ligands, has been addressed in studies carried out on the CO, F(-), OH(-), CN(-), and HS(-) adducts formed with the wild-type protein and a combinatorial set of mutants, in which the distal polar residues, TrpG8, TyrCD1, and TyrB10, have been singly, doubly, or triply replaced by a Phe residue. In this context, such a complete analysis provides an excellent benchmark for the investigation of the relationship between protein structure and function, allowing one to translate physicochemical properties of the active site into the observed functional behaviour. Tf-trHb will be compared with other members of the group II trHbs and, more generally, with members of the other trHb subgroups. © 2015 Elsevier Ltd. All rights reserved.

  14. Expression, Characterization and Synergistic Interactions of Myxobacter Sp. AL-1 Cel9 and Cel48 Glycosyl Hydrolases

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    Mario Pedraza-Reyes

    2008-02-01

    Full Text Available The soil microorganism Myxobacter Sp. AL-1 regulates in a differential manner the production of five extracellular cellulases during its life cycle. The nucleotide sequence of a cel9-cel48 cluster from the genome of this microorganism was recently obtained. Cel48 was expressed in Escherichia coli to generate a His6-Cel48 protein and the biochemical properties of the pure protein were determined. Cel48 was more efficient in degrading acid-swollen avicel (ASC than carboxymethylcellulose (CMC. On the other hand, cel9 was expressed in Bacillus subtilis from an IPTG-inducible promoter. Zymogram analysis showed that after IPTG-induction, Cel9 existed in both the cell fraction and the culture medium of B. subtilis and the secreted protein was purified to homogeneity by FPLC-ionic exchange chromatography. The exocellobiohydrolase Cel48 showed a synergism of 1.68 times with the endocellulase Cel9 during ASC degradation using an 8.1- fold excess of Cel48 over Cel9. Western blot analysis revealed that both proteins were synthesized and secreted to the culture medium of Myxobacter Sp. AL-1. These results show that the cel9-cel48 cluster encodes functional endo- and exo-acting cellulases that allows Myobacter Sp. AL-1 to hydrolyse cellulose.

  15. Molecular and biochemical analyses of CbCel9A/Cel48A, a highly secreted multi-modular cellulase by Caldicellulosiruptor bescii during growth on crystalline cellulose.

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    Zhuolin Yi

    Full Text Available During growth on crystalline cellulose, the thermophilic bacterium Caldicellulosiruptor bescii secretes several cellulose-degrading enzymes. Among these enzymes is CelA (CbCel9A/Cel48A, which is reported as the most highly secreted cellulolytic enzyme in this bacterium. CbCel9A/Cel48A is a large multi-modular polypeptide, composed of an N-terminal catalytic glycoside hydrolase family 9 (GH9 module and a C-terminal GH48 catalytic module that are separated by a family 3c carbohydrate-binding module (CBM3c and two identical CBM3bs. The wild-type CbCel9A/Cel48A and its truncational mutants were expressed in Bacillus megaterium and Escherichia coli, respectively. The wild-type polypeptide released twice the amount of glucose equivalents from Avicel than its truncational mutant that lacks the GH48 catalytic module. The truncational mutant harboring the GH9 module and the CBM3c was more thermostable than the wild-type protein, likely due to its compact structure. The main hydrolytic activity was present in the GH9 catalytic module, while the truncational mutant containing the GH48 module and the three CBMs was ineffective in degradation of either crystalline or amorphous cellulose. Interestingly, the GH9 and/or GH48 catalytic modules containing the CBM3bs form low-density particles during hydrolysis of crystalline cellulose. Moreover, TM3 (GH9/CBM3c and TM2 (GH48 with three CBM3 modules synergistically hydrolyze crystalline cellulose. Deletion of the CBM3bs or mutations that compromised their binding activity suggested that these CBMs are important during hydrolysis of crystalline cellulose. In agreement with this observation, seven of nine genes in a C. bescii gene cluster predicted to encode cellulose-degrading enzymes harbor CBM3bs. Based on our results, we hypothesize that C. bescii uses the GH48 module and the CBM3bs in CbCel9A/Cel48A to destabilize certain regions of crystalline cellulose for attack by the highly active GH9 module and other

  16. Molecular and biochemical analyses of CbCel9A/Cel48A, a highly secreted multi-modular cellulase by Caldicellulosiruptor bescii during growth on crystalline cellulose.

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    Yi, Zhuolin; Su, Xiaoyun; Revindran, Vanessa; Mackie, Roderick I; Cann, Isaac

    2013-01-01

    During growth on crystalline cellulose, the thermophilic bacterium Caldicellulosiruptor bescii secretes several cellulose-degrading enzymes. Among these enzymes is CelA (CbCel9A/Cel48A), which is reported as the most highly secreted cellulolytic enzyme in this bacterium. CbCel9A/Cel48A is a large multi-modular polypeptide, composed of an N-terminal catalytic glycoside hydrolase family 9 (GH9) module and a C-terminal GH48 catalytic module that are separated by a family 3c carbohydrate-binding module (CBM3c) and two identical CBM3bs. The wild-type CbCel9A/Cel48A and its truncational mutants were expressed in Bacillus megaterium and Escherichia coli, respectively. The wild-type polypeptide released twice the amount of glucose equivalents from Avicel than its truncational mutant that lacks the GH48 catalytic module. The truncational mutant harboring the GH9 module and the CBM3c was more thermostable than the wild-type protein, likely due to its compact structure. The main hydrolytic activity was present in the GH9 catalytic module, while the truncational mutant containing the GH48 module and the three CBMs was ineffective in degradation of either crystalline or amorphous cellulose. Interestingly, the GH9 and/or GH48 catalytic modules containing the CBM3bs form low-density particles during hydrolysis of crystalline cellulose. Moreover, TM3 (GH9/CBM3c) and TM2 (GH48 with three CBM3 modules) synergistically hydrolyze crystalline cellulose. Deletion of the CBM3bs or mutations that compromised their binding activity suggested that these CBMs are important during hydrolysis of crystalline cellulose. In agreement with this observation, seven of nine genes in a C. bescii gene cluster predicted to encode cellulose-degrading enzymes harbor CBM3bs. Based on our results, we hypothesize that C. bescii uses the GH48 module and the CBM3bs in CbCel9A/Cel48A to destabilize certain regions of crystalline cellulose for attack by the highly active GH9 module and other endoglucanases

  17. Genomics of aerobic cellulose utilization systems in actinobacteria.

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    Iain Anderson

    Full Text Available Cellulose degrading enzymes have important functions in the biotechnology industry, including the production of biofuels from lignocellulosic biomass. Anaerobes including Clostridium species organize cellulases and other glycosyl hydrolases into large complexes known as cellulosomes. In contrast, aerobic actinobacteria utilize systems comprised of independently acting enzymes, often with carbohydrate binding domains. Numerous actinobacterial genomes have become available through the Genomic Encyclopedia of Bacteria and Archaea (GEBA project. We identified putative cellulose-degrading enzymes belonging to families GH5, GH6, GH8, GH9, GH12, GH48, and GH51 in the genomes of eleven members of the actinobacteria. The eleven organisms were tested in several assays for cellulose degradation, and eight of the organisms showed evidence of cellulase activity. The three with the highest cellulase activity were Actinosynnema mirum, Cellulomonas flavigena, and Xylanimonas cellulosilytica. Cellobiose is known to induce cellulolytic enzymes in the model organism Thermobifida fusca, but only Nocardiopsis dassonvillei showed higher cellulolytic activity in the presence of cellobiose. In T. fusca, cellulases and a putative cellobiose ABC transporter are regulated by the transcriptional regulator CelR. Nine organisms appear to use the CelR site or a closely related binding site to regulate an ABC transporter. In some, CelR also regulates cellulases, while cellulases are controlled by different regulatory sites in three organisms. Mining of genome data for cellulose degradative enzymes followed by experimental verification successfully identified several actinobacteria species which were not previously known to degrade cellulose as cellulolytic organisms.

  18. Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum

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    Najmudin, Shabir; Guerreiro, Catarina I. P. D.; Ferreira, Luís M. A.; Romão, Maria J. C.; Fontes, Carlos M. G. A.; Prates, José A. M.

    2005-01-01

    The two C-terminal domains of the cellulase ctCel9D-Cel44A from C. thermocellum cellulosome have been crystallized in tetragonal space group P4 3 2 1 2 and X-ray diffraction data have been collected to 2.1 and 2.8 Å from native and seleno-l-methionine-derivative crystals, respectively. Clostridium thermocellum produces a highly organized multi-enzyme complex of cellulases and hemicellulases for the hydrolysis of plant cell-wall polysaccharides, which is termed the cellulosome. The bifunctional multi-modular cellulase ctCel9D-Cel44A is one of the largest components of the C. thermocellum cellulosome. The enzyme contains two internal catalytic domains belonging to glycoside hydrolase families 9 and 44. The C-terminus of this cellulase, comprising a polycystic kidney-disease module (PKD) and a carbohydrate-binding module (CBM44), has been crystallized. The crystals belong to the tetragonal space group P4 3 2 1 2, containing a single molecule in the asymmetric unit. Native and seleno-l-methionine-derivative crystals diffracted to 2.1 and 2.8 Å, respectively

  19. Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum

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    Najmudin, Shabir [REQUIMTE, Departamento de Química, FCT-UNL, 2829-516 Caparica (Portugal); Guerreiro, Catarina I. P. D.; Ferreira, Luís M. A. [CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa (Portugal); Romão, Maria J. C. [REQUIMTE, Departamento de Química, FCT-UNL, 2829-516 Caparica (Portugal); Fontes, Carlos M. G. A.; Prates, José A. M., E-mail: japrates@fmv.utl.pt [CIISA - Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa (Portugal); REQUIMTE, Departamento de Química, FCT-UNL, 2829-516 Caparica (Portugal)

    2005-12-01

    The two C-terminal domains of the cellulase ctCel9D-Cel44A from C. thermocellum cellulosome have been crystallized in tetragonal space group P4{sub 3}2{sub 1}2 and X-ray diffraction data have been collected to 2.1 and 2.8 Å from native and seleno-l-methionine-derivative crystals, respectively. Clostridium thermocellum produces a highly organized multi-enzyme complex of cellulases and hemicellulases for the hydrolysis of plant cell-wall polysaccharides, which is termed the cellulosome. The bifunctional multi-modular cellulase ctCel9D-Cel44A is one of the largest components of the C. thermocellum cellulosome. The enzyme contains two internal catalytic domains belonging to glycoside hydrolase families 9 and 44. The C-terminus of this cellulase, comprising a polycystic kidney-disease module (PKD) and a carbohydrate-binding module (CBM44), has been crystallized. The crystals belong to the tetragonal space group P4{sub 3}2{sub 1}2, containing a single molecule in the asymmetric unit. Native and seleno-l-methionine-derivative crystals diffracted to 2.1 and 2.8 Å, respectively.

  20. Obtención de Variantes Hiperactivas e Inactivas de la Endocelulasa Cel9 de Myxobacter Sp. Al-1 Obtención de Variantes Hiperactivas e Inactivas de la Endocelulasa Cel9 de Myxobacter Sp. Al-1

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    Mario Pedraza-Reyes

    2012-02-01

    Full Text Available Debido a su aplicación industrial, existe un gran interés en la producción de celulasas con propiedades bioquímicas novedosas. Por ello, en el presente trabajo se utilizó una estrategia basada en un método de mutagénesis aleatoria in vivo para la obtención de variantes de la endocelulasa Cel9 del microorganismo gram-negativo Myxobacter Sp. AL-1. Siguiendo este enfoque, se obtuvieron cepas transformantes de Escherichia coli capaces de secretar variantes de la proteína Cel9 cuyas actividades específicas fueron incrementadas hasta 7.5 veces con respecto a la actividad mostrada por la enzima nativa. Del mismo modo, se generaron cepas de E. coli productoras de variantes de la proteína Cel9 con baja o nula actividad enzimática. Experimentos de subclonación y fraccionamiento celular revelaron que las mutaciones asociadas con los fenotipos de las variantes de la enzima Cel9 ocurrieron en la secuencia del gen cel9. Así mismo, se demostró que los fenotipos de las cepas mutantes carentes de actividad enzimática no dependen de su incapacidad para secretar las proteínas mutantes. Además de su potencial aplicación biotecnológica, los resultados obtenidos en este trabajo permiten avanzar en el entendimiento de la relación estructura-función de la celulasa Cel9 de Myxobacter Sp. AL-1.Due to its biotechnological impact, there is currently a growing interest in the production of cellulases with novel biochemical properties. Here, multiple generations of random mutagenesis in vivo and screening were employed to generate variants of the modular cellulase Cel9 from Myxobacter Sp. AL-1. Following this approach, Cel9 variants which showed increases upto 7.5 fold of cellulase activity were obtained. In addition, Cel9 mutants which completely lost the ability to degrade cellulose were also obtained. Results revealed that mutations associated with the phenotype of the Cel9 variants occurred on the mutant gene sequence and that themutants with null

  1. [Gohieria fusca found in dust of air-conditioner filters].

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    Qiang, Chai; Xiao-Dong, Zhan; Wei, Guo; Chao-Pin, Li

    2017-09-25

    To investigate the pollution status of Gohieria fusca in the air conditioner-filters of different places in Wuhu City. The dust samples were collected from the filters of air-conditioners in dining rooms, shopping malls, hotels and households between June and September, 2013, and G. fusca was detected in the dust samples. There were 430 dust samples collected and 98 were G. fusca positive with the breeding rate of 22.79%. The difference of breeding rates of G. fusca were statistically significant among the different places ( χ 2 =18.294, P air-conditioner filters in Wuhu City gravely.

  2. Intracellular β-Glucosidases CEL1a and CEL1b Are Essential for Cellulase Induction on Lactose in Trichoderma reesei

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    Xu, Jintao; Zhao, Guolei; Kou, Yanbo; Zhang, Weixin; Zhou, Qingxin; Chen, Guanjun

    2014-01-01

    Lactose (1,4-O-β-d-galacto-pyranosyl-d-glucose) induces cellulolytic enzymes in Trichoderma reesei and is in fact one of the most important soluble carbon sources used to produce cellulases on an industrial level. The mechanism underlying the induction is, however, not fully understood. In this study, we investigated the cellular functions of the intracellular β-glucosidases CEL1a and CEL1b in the induction of cellulase genes by lactose in T. reesei. We demonstrated that while CEL1a and CEL1b were functionally equivalent in mediating the induction, the simultaneous absence of these intracellular β-glucosidases abolished cbh1 gene expression on lactose. d-Galactose restored the efficient cellulase gene induction in the Δcel1a strain independently of its reductive metabolism, but not in the Δcel1a Δcel1b strain. A further comparison of the transcriptional responses of the Δcel1a Δcel1b strain complemented with wild-type CEL1a or a catalytically inactive CEL1a version and the Δcel1a strain constitutively expressing CEL1a or the Kluyveromyces lactis β-galactosidase LAC4 showed that both the CEL1a protein and its glycoside hydrolytic activity were indispensable for cellulase induction by lactose. We also present evidence that intracellular β-glucosidase-mediated lactose induction is further conveyed to XYR1 to ensure the efficiently induced expression of cellulase genes. PMID:24879125

  3. Molecular Dynamics and Metadynamics Simulations of the Cellulase Cel48F

    Directory of Open Access Journals (Sweden)

    Osmair Vital de Oliveira

    2014-01-01

    Full Text Available Molecular dynamics (MD and metadynamics techniques were used to study the cellulase Cel48F-sugar. Cellulase is enzyme that breaks cellulose fibers into small sugar units and is potentially useful in second generation alcohol production. In MD simulations, the overall structure of equilibrated Cel48F did not significantly change along the trajectory, retaining root mean square deviation below 0.15 nm. A set of 15 residues interacting with the sugar chains via hydrogen bonding throughout the simulation was observed. The free energy of dissociation (ΔGdiss. of the chains in the catalytic tunnel of Cel48F was determined by metadynamics. The ΔGdiss. values of the chains entering and leaving the wild-type Cel48F cavity were 13.9 and 62.1 kcal/mol, respectively. We also mutated the E542 and Q543 to alanine residue and obtained ΔGdiss. of 41.8 and 45.9 kcal/mol, respectively. These mutations were found to facilitate smooth dissociation of the sugar chain across the Cel48F tunnel. At the entry of the Cel48F tunnel, three residues were mutated to alanine: T110, T213, and L274. Contrary to the T110A-Cel48F, the mutants T213-Cel48F and L274-Cel48F prevented the sugar chain from passing across the leaving site. The present results can be a guideline in mutagenesis studies to improve processing by Cel48F.

  4. An autoantibody against Nε-(carboxyethyl)lysine (CEL): Possible involvement in the removal of CEL-modified proteins by macrophages

    International Nuclear Information System (INIS)

    Mera, Katsumi; Nagai, Ryoji; Takeo, Kazuhiro; Izumi, Miyoko; Maruyama, Toru; Otagiri, Masaki

    2011-01-01

    Highlights: → A higher amount of autoantibody against CEL than that of other AGEs was observed in human plasma. → The purified human anti-CEL autoantibody specifically reacted with CEL. → Anti-CEL antibody accelerated the uptake of 125 I-CEL-HSA by macrophage in vitro. → Endocytic uptake of 125 I-CEL-HSA by mice liver was accelerated in the presence of anti-CEL antibody. -- Abstract: Advanced glycation end products (AGEs) are believed to play a significant role in the development of diabetic complications. In this study, we measured the levels of autoantibodies against several AGE structures in healthy human plasma and investigated the physiological role of the autoantibodies. A high titer of the autoantibody against N ε -(carboxyethyl)lysine (CEL) was detected in human plasma compared with other AGE structures such as CML and pentosidine. The purified human anti-CEL autoantibody reacted with CEL-modified human serum albumin (CEL-HSA), but not CML-HSA. A rabbit polyclonal anti-CEL antibody, used as a model autoantibody against CEL, accelerated the uptake of CEL-HSA by macrophages, but did not enhance the uptake of native HSA. Furthermore, when 125 I-labeled CEL-HSA was injected into the tail vein of mice, accumulation of 125 I-CEL-HSA in the liver was accelerated by co-injection of the rabbit anti-CEL antibody. These results demonstrate that the autoantibody against CEL in plasma may play a role in the macrophage uptake of CEL-modified proteins.

  5. Exo-exo synergy between Cel6A and Cel7A from Hypocrea jecorina

    DEFF Research Database (Denmark)

    Badino, Silke Flindt; Christensen, Stefan Jarl; Kari, Jeppe

    2017-01-01

    Synergy between cellulolytic enzymes is essential in both natural and industrial breakdown of biomass. In addition to synergy between endo- and exo-lytic enzymes, a lesser known but equally conspicuous synergy occurs among exo-acting, processive cellobiohydrolases (CBHs) such as Cel7A and Cel6A...... from Hypocrea jecorina. We studied this system using microcrystalline cellulose as substrate and found a degree of synergy between 1.3 and 2.2 depending on the experimental conditions. Synergy between enzyme variants without the carbohydrate binding module (CBM) and its linker was strongly reduced...... compared to the wild types. One plausible interpretation of this is that exo-exo synergy depends on the targeting role of the CBM. Many earlier works have proposed that exo-exo synergy was caused by an auxiliary endo-lytic activity of Cel6A. However, biochemical data from different assays suggested...

  6. Identification of the C-Terminal GH5 Domain from CbCel9B/Man5A as the First Glycoside Hydrolase with Thermal Activation Property from a Multimodular Bifunctional Enzyme.

    Directory of Open Access Journals (Sweden)

    Rong Wang

    Full Text Available Caldicellulosiruptor bescii encodes at least six unique multimodular glycoside hydrolases crucial for plant cell wall polysaccharides degradation, with each having two catalytic domains separated by two to three carbohydrate binding modules. Among the six enzymes, three have one N- or C-terminal GH5 domain with identical amino acid sequences. Despite a few reports on some of these multimodular enzymes, little is known about how the conserved GH5 domains behave, which are believed to be important due to the gene duplication. We thus cloned a representative GH5 domain from the C-terminus of a multimodular protein, i.e. the bifunctional cellulase/mannanase CbCel9B/Man5A which has been reported, and expressed it in Escherichia coli. Without any appending CBMs, the recombinant CbMan5A was still able to hydrolyze a variety of mannan substrates with different backbone linkages or side-chain decorations. While CbMan5A displayed the same pH optimum as CbCel9B/Man5A, it had an increased optimal temperature (90°C and moreover, was activated by heating at 70°C and 80°C, a property not ever reported for the full-length protein. The turnover numbers of CbMan5A on mannan substrates were, however, lower than those of CbCel9B/Man5A. These data suggested that evolution of CbMan5A and the other domains into a single polypeptide is not a simple assembly; rather, the behavior of one module may be affected by the other ones in the full-length enzyme. The differential scanning calorimetry analysis further indicated that heating CbMan5A was not a simple transition state process. To the best knowledge of the authors, CbMan5A is the first glycoside hydrolase with thermal activation property identified from a multimodular bifunctional enzyme.

  7. Bioaccumulation and Depuration of Copper in the Kidney and Liver of a Freshwater Fish, Capoeta fusca

    Directory of Open Access Journals (Sweden)

    Borhan Mansouri

    2016-07-01

    Full Text Available Background: This study aims to investigate the patterns of bioaccumulation and depuration of copper in the selected kidney and liver of Capoeta fusca. Methods: The fish were collected between September and November 2010 from a qanat in Birjand. They were exposed to two types treatments with copper (0.25 and 0.75 mg/L for a period of 41 days. The fish under study were exposed to the above-mentioned sub-lethal concentrations separately for 14 and 21 days (accumulation period. At the end of this period, the remaining fish were kept in tap water (elimination period for 31 and 41 days. Results: The findings showed that the accumulation of copper in lower and higher sub-lethal concentrations was higher in kidney as the mean accumulation of copper on day 21 was 1.9±0.1 μg/g and 2.93±0.47 μg/g respectively, in 0.25 μg/g and 0.75 μg/g concentrations. On the other hand, the results also showed that the depuration level of copper in the given concentrations was higher in liver than kidney. The bioaccumulation and depuration of copper significantly increased in the kidney and liver of C. fusca (P<0.01. Conclusion: Based on the present work, it is concluded that C. fusca has a potential for the rapid accumulation and depuration of copper in freshwater. Also, the results indicate that the fish C. fusca, as representative fish species in the East of Iran, can be a useful bioindicator organism of water contamination with copper.

  8. Direct kinetic comparison of the two cellobiohydrolases Cel6A and Cel7A from Hypocrea jecorina

    DEFF Research Database (Denmark)

    Badino, Silke Flindt; Kari, Jeppe; Christensen, Stefan Jarl

    2017-01-01

    Cellulose degrading fungi such as Hypocrea jecorina secrete several cellulases including the two cellobiohydrolases (CBHs) Cel6A and Cel7A. The two CBHs differ in catalytic mechanism, attack different ends, belong to different families, but are both processive multi-domain enzymes that are essent...

  9. An autoantibody against N{sup {epsilon}}-(carboxyethyl)lysine (CEL): Possible involvement in the removal of CEL-modified proteins by macrophages

    Energy Technology Data Exchange (ETDEWEB)

    Mera, Katsumi [Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto (Japan); Nagai, Ryoji, E-mail: nagai-883@umin.ac.jp [Department of Food and Nutrition, Laboratory of Nutritional Science and Biochemistry, Japan Women' s University, Tokyo (Japan); Takeo, Kazuhiro; Izumi, Miyoko [Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto (Japan); Maruyama, Toru [Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto (Japan); Center for Clinical Pharmaceutical Science, Kumamoto University, Kumamoto (Japan); Otagiri, Masaki [Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto (Japan); Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto (Japan)

    2011-04-08

    Highlights: {yields} A higher amount of autoantibody against CEL than that of other AGEs was observed in human plasma. {yields} The purified human anti-CEL autoantibody specifically reacted with CEL. {yields} Anti-CEL antibody accelerated the uptake of {sup 125}I-CEL-HSA by macrophage in vitro. {yields} Endocytic uptake of {sup 125}I-CEL-HSA by mice liver was accelerated in the presence of anti-CEL antibody. -- Abstract: Advanced glycation end products (AGEs) are believed to play a significant role in the development of diabetic complications. In this study, we measured the levels of autoantibodies against several AGE structures in healthy human plasma and investigated the physiological role of the autoantibodies. A high titer of the autoantibody against N{sup {epsilon}}-(carboxyethyl)lysine (CEL) was detected in human plasma compared with other AGE structures such as CML and pentosidine. The purified human anti-CEL autoantibody reacted with CEL-modified human serum albumin (CEL-HSA), but not CML-HSA. A rabbit polyclonal anti-CEL antibody, used as a model autoantibody against CEL, accelerated the uptake of CEL-HSA by macrophages, but did not enhance the uptake of native HSA. Furthermore, when {sup 125}I-labeled CEL-HSA was injected into the tail vein of mice, accumulation of {sup 125}I-CEL-HSA in the liver was accelerated by co-injection of the rabbit anti-CEL antibody. These results demonstrate that the autoantibody against CEL in plasma may play a role in the macrophage uptake of CEL-modified proteins.

  10. Exo-exo synergy between Cel6A and Cel7A from Hypocrea jecorina: Role of carbohydrate binding module and the endo-lytic character of the enzymes.

    Science.gov (United States)

    Badino, Silke F; Christensen, Stefan J; Kari, Jeppe; Windahl, Michael S; Hvidt, Søren; Borch, Kim; Westh, Peter

    2017-08-01

    Synergy between cellulolytic enzymes is essential in both natural and industrial breakdown of biomass. In addition to synergy between endo- and exo-lytic enzymes, a lesser known but equally conspicuous synergy occurs among exo-acting, processive cellobiohydrolases (CBHs) such as Cel7A and Cel6A from Hypocrea jecorina. We studied this system using microcrystalline cellulose as substrate and found a degree of synergy between 1.3 and 2.2 depending on the experimental conditions. Synergy between enzyme variants without the carbohydrate binding module (CBM) and its linker was strongly reduced compared to the wild types. One plausible interpretation of this is that exo-exo synergy depends on the targeting role of the CBM. Many earlier works have proposed that exo-exo synergy was caused by an auxiliary endo-lytic activity of Cel6A. However, biochemical data from different assays suggested that the endo-lytic activity of both Cel6A and Cel7A were 10 3 -10 4 times lower than the common endoglucanase, Cel7B, from the same organism. Moreover, the endo-lytic activity of Cel7A was 2-3-fold higher than for Cel6A, and we suggest that endo-like activity of Cel6A cannot be the main cause for the observed synergy. Rather, we suggest the exo-exo synergy found here depends on different specificities of the enzymes possibly governed by their CBMs. Biotechnol. Bioeng. 2017;114: 1639-1647. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  11. Biological CO2 mitigation from coal power plant by Chlorella fusca and Spirulina sp.

    Science.gov (United States)

    Duarte, Jessica Hartwig; de Morais, Etiele Greque; Radmann, Elisângela Martha; Costa, Jorge Alberto Vieira

    2017-06-01

    CO 2 biofixation by microalgae and cyanobacteria is an environmentally sustainable way to mitigate coal burn gas emissions. In this work the microalga Chlorella fusca LEB 111 and the cyanobacteria Spirulina sp. LEB 18 were cultivated using CO 2 from coal flue gas as a carbon source. The intermittent flue gas injection in the cultures enable the cells growth and CO 2 biofixation by these microorganisms. The Chlorella fusca isolated from a coal power plant could fix 2.6 times more CO 2 than Spirulina sp. The maximum daily CO 2 from coal flue gas biofixation was obtained with Chlorella fusca (360.12±0.27mgL -1 d -1 ), showing a specific growth rate of 0.17±<0.01d -1 . The results demonstrated the Chlorella fusca LEB 111 and Spirulina sp. LEB 18 potential to fix CO 2 from coal flue gas, and sequential biomass production with different biotechnological destinations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea.

    Science.gov (United States)

    Park, Sooyeon; Jung, Yong-Taek; Kang, Chul-Hyung; Park, Ja-Min; Yoon, Jung-Hoon

    2014-11-01

    A Gram-stain-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated GJSW-36(T), was isolated from seawater at Geoje island in the South Sea, South Korea. Strain GJSW-36(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GJSW-36(T) fell within the clade comprising the type strains of species of the genus Thalassotalea and Thalassomonas fusca. Strain GJSW-36(T) exhibited 16S rRNA gene sequence similarity values of 94.2-96.0% to the type strains of species of the genus Thalassotalea and Thalassomonas fusca and of 93.8-94.5% to the type strains of the other species of the genus Thalassomonas. Strain GJSW-36(T) contained ubiquinone-8 (Q-8) as the predominant ubiquinone and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(1:1)ω8c and C(16:0) as the major fatty acids. The major polar lipids of strain GJSW-36(T) were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain GJSW-36(T) was 45.1 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GJSW-36(T) is separated from species of the genus Thalassotalea and Thalassomonas fusca. On the basis of the data presented, strain GJSW-36(T) is considered to represent a novel species of the genus Thalassotalea, for which the name Thalassotalea ponticola sp. nov. is proposed. The type strain is GJSW-36(T) ( =KCTC 42155(T) =CECT 8656(T)). From this study, it is also proposed that Thalassomonas fusca should be reclassified as a member of the genus Thalassotalea and the description of the genus Thalassotalea is emended. © 2014 IUMS.

  13. Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design.

    Directory of Open Access Journals (Sweden)

    Jinfeng Zhang

    Full Text Available To meet the demand for the application of high activity and thermostable cellulases in the production of new-generation bioethanol from nongrain-cellulose sources, a hyperthermostable β-1,4-endoglucase Cel12B from Thermotoga maritima was selected for further modification by gene site-directed mutagenesis method in the present study, based on homology modeling and rational design. As a result, two recombinant enzymes showed significant improvement in enzyme activity by 77% and 87%, respectively, higher than the parental enzyme TmCel12B. Furthermore, the two mutants could retain 80% and 90.5% of their initial activity after incubation at 80°C for 8 h, while only 45% for 5 h to TmCel12B. The Km and Vmax of the two recombinant enzymes were 1.97±0.05 mM, 4.23±0.15 μmol·mg(-1·min(-1 of TmCel12B-E225H-K207G-D37V, and 2.97±0.12 mM, 3.15±0.21 μmol·mg(-1·min(-1 of TmCel12B-E225H-K207G, respectively, when using CMC-Na as the substrate. The roles of the mutation sites were also analyzed and evaluated in terms of electron density, hydrophobicity of the modeled protein structures. The recombinant enzymes may be used in the hydrolysis of cellulose at higher temperature in the future. It was concluded that the gene mutagenesis approach of a certain active residues may effectively improve the performance of cellulases for the industrial applications and contribute to the study the thermostable mechanism of thermophilic enzymes.

  14. Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase.

    Science.gov (United States)

    Pierce, Brian C; Agger, Jane Wittrup; Wichmann, Jesper; Meyer, Anne S

    2017-03-01

    The auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenase (LPMO) from Trichoderma reesei (EG4; TrCel61A) was investigated for its ability to oxidize the complex polysaccharides from soybean. The substrate specificity of the enzyme was assessed against a variety of substrates, including both soy spent flake, a by-product of the soy food industry, and soy spent flake pretreated with sodium hydroxide. Products from enzymatic treatments were analyzed using mass spectrometry and high performance anion exchange chromatography. We demonstrate that TrCel61A is capable of oxidizing cellulose from both pretreated soy spent flake and phosphoric acid swollen cellulose, oxidizing at both the C1 and C4 positions. In addition, we show that the oxidative activity of TrCel61A displays a synergistic effect capable of boosting endoglucanase activity, and thereby substrate depolymerization of soy cellulose, by 27%. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Bactericidal and cytotoxic effects of Erythrina fusca leaves aquadest extract

    Directory of Open Access Journals (Sweden)

    Janti Sudiono

    2013-03-01

    Full Text Available Background: Empirically, Erythrina fusca has been used as traditional herb for its antibacterial and antiinflammation properties. Periodontal disease is one of the most oral infectious diseases with microorganism predominated as the contributing factors. Porphyromonas gingivalis (P. gingivalis is one of the main bacteria pathogen found in periodontal diseases. Purpose: The purpose of this study was to examine the bactericidal effect of Erythrina fusca Leaves Aquadest Extract (EFLAE at various concentrations on P. gingivalis and cytotoxic effect on fibroblast. Methods: Pure P. gingivalis was cultured in Brain Heart Infusion (BHI medium for 24 hours with or without various concentrations of treatment of EFLAE. Calculation and statistical analysis of remaining bacteria were performed by inhibitory zone method to evaluate the EFLAE bactericidal effect and compared to chlorhexidine as positive control. To evaluate the cytotoxic effect, NIH 3T3 cells were cultured in Dulbecco’s Modification of Eagle’s Medium (DMEM containing of 10% fetal bovine serum (FBS and 1% penicillin-streptomycin, pH 7.2, in 5% CO2, and stored in humidified incubator under temperature 370 C. Cells were treated with/without various concentrations of EFLAE for 48 hours. The viable cells were then counted using 3-(4,5- Dimethylthiazol-2-yl-2,5 diphenyl tetrazodium bromide (MTT method. Results: EFLAE have bactericidal effect on P. gingivalis in a concentration dependent manner starting from 78%. The concentration of 90% EFLAE had stronger bactericidal effect (35.004 ± 1.546 than those of chlorhexidine as positive control (32.313 ± 1.619. One-way ANOVA showed significant bactericidal effect differences among concentrations of EFLAE and chlorhexidine (p<0.05 while Tuckey HSD test showed significant difference only between lower concentration of EFLAE (78%, 79% and chlorhexidine. With the highest concentration of EFLAE (100% applied in the bactericidal test, no cytotoxic effect

  16. Gas exchange patterns and water loss rates in the Table Mountain cockroach, Aptera fusca (Blattodea: Blaberidae).

    Science.gov (United States)

    Groenewald, Berlizé; Bazelet, Corinna S; Potter, C Paige; Terblanche, John S

    2013-10-15

    The importance of metabolic rate and/or spiracle modulation for saving respiratory water is contentious. One major explanation for gas exchange pattern variation in terrestrial insects is to effect a respiratory water loss (RWL) saving. To test this, we measured the rates of CO2 and H2O release ( and , respectively) in a previously unstudied, mesic cockroach, Aptera fusca, and compared gas exchange and water loss parameters among the major gas exchange patterns (continuous, cyclic, discontinuous gas exchange) at a range of temperatures. Mean , and per unit did not differ among the gas exchange patterns at all temperatures (P>0.09). There was no significant association between temperature and gas exchange pattern type (P=0.63). Percentage of RWL (relative to total water loss) was typically low (9.79±1.84%) and did not differ significantly among gas exchange patterns at 15°C (P=0.26). The method of estimation had a large impact on the percentage of RWL, and of the three techniques investigated (traditional, regression and hyperoxic switch), the traditional method generally performed best. In many respects, A. fusca has typical gas exchange for what might be expected from other insects studied to date (e.g. , , RWL and cuticular water loss). However, we found for A. fusca that expressed as a function of metabolic rate was significantly higher than the expected consensus relationship for insects, suggesting it is under considerable pressure to save water. Despite this, we found no consistent evidence supporting the conclusion that transitions in pattern type yield reductions in RWL in this mesic cockroach.

  17. 42 CFR 90.9 - Public health advisory.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Public health advisory. 90.9 Section 90.9 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH... PROCEDURES § 90.9 Public health advisory. ATSDR may issue a public health advisory based on the findings of a...

  18. Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase

    DEFF Research Database (Denmark)

    Pierce, Brian; Wittrup Agger, Jane; Wichmann, Jesper

    2017-01-01

    The auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenase (LPMO) from Trichoderma reesei (EG4; TrCel61A) was investigated for its ability to oxidize the complex polysaccharides from soybean. The substrate specificity of the enzyme was assessed against a variety of ...

  19. 9 CFR 381.90 - Cadavers.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Cadavers. 381.90 Section 381.90 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY... § 381.90 Cadavers. Carcasses of poultry showing evidence of having died from causes other than slaughter...

  20. Control of Busseola fusca and Chilo partellus stem borers by ...

    African Journals Online (AJOL)

    Previous testing of several public Bacillus thuringiensis (Bt)-maize events did not show control of the African stem borer (Busseola fusca Fuller), an important stem borer species, without which stewardship would be compromised by the possibility of rapid development of resistance to Bt deltaendotoxins. This study was ...

  1. Mutations in the VNTR of the carboxyl-ester lipase gene (CEL) are a rare cause of monogenic diabetes

    DEFF Research Database (Denmark)

    Torsvik, Janniche; Johansson, Stefan; Johansen, Anders

    2009-01-01

    of the VNTR, and determined the VNTR-length of each allele. When blindly testing 56 members of the two families with known single-base deletions in the CEL VNTR, the method correctly assessed the mutation carriers. Screening of 241 probands from suspected maturity-onset diabetes of the young (MODY) families...... negative for mutations in known MODY genes (95 individuals from Denmark and 146 individuals from UK) revealed no deletions in the proximal repeats of the CEL VNTR. However, we found one Danish patient with a short, novel CEL allele containing only three VNTR repeats (normal range 7-23 in healthy controls......). This allele co-segregated with diabetes or impaired glucose tolerance in the patient's family as six of seven mutation carriers were affected. We also identified individuals who had three copies of a complete CEL VNTR. In conclusion, the CEL gene is highly polymorphic, but mutations in CEL are likely...

  2. Xylan oligosaccharides and cellobiohydrolase I (TrCel7A interaction and effect on activity

    Directory of Open Access Journals (Sweden)

    Baumann Martin J

    2011-10-01

    Full Text Available Abstract Background The well-studied cellulase mixture secreted by Trichoderma reesei (anamorph to Hypocrea jecorina contains two cellobiohydolases (CBHs, cellobiohydrolase I (TrCel7A and cellobiohydrolase II (TrCeI6A, that are core enzymes for the solubilisation of cellulose. This has attracted significant research interest because of the role of the CBHs in the conversion of biomass to fermentable sugars. However, the CHBs are notoriously slow and susceptible to inhibition, which presents a challenge for the commercial utilisation of biomass. The xylans and xylan fragments that are also present in the biomass have been suggested repeatedly as one cause of the reduced activity of CHBs. Yet, the extent and mechanisms of this inhibition remain poorly elucidated. Therefore, we studied xylan oligosaccharides (XOSs of variable lengths with respect to their binding and inhibition of both TrCel7A and an enzyme variant without the cellulose-binding domain (CBM. Results We studied the binding of XOSs to TrCel7A by isothermal titration calorimetry. We found that XOSs bind to TrCel7A and that the affinity increases commensurate with XOS length. The CBM, on the other hand, did not affect the affinity significantly, which suggests that XOSs may bind to the active site. Activity assays of TrCel7A clearly demonstrated the negative effect of the presence of XOSs on the turnover number. Conclusions On the basis of these binding data and a comparison of XOS inhibition of the activity of the two enzyme variants towards, respectively, soluble and insoluble substrates, we propose a competitive mechanism for XOS inhibition of TrCel7A with phosphoric swollen cellulose as a substrate.

  3. Effects of pine pollen supplementation in an onion diet on Frankliniella fusca reproduction.

    Science.gov (United States)

    Angelella, G M; Riley, D G

    2010-04-01

    A micro-cage bioassay was developed to test the effect of slash pine pollen (Pinus elliottii Engelm.) supplementation to a whole onion plant (Allium cepa L. variety Pegasus) diet on thrips (Thysanoptera: Thripidae) reproductive parameters. Frankliniella fusca (Hinds) females were placed on two to three-leaf stage onion seedling under a treatment of either slash pine (Pinus elliottii Engelm.) pollen dusting (a pollen supplement) or no pollen treatment. Adult survival, net oviposition, and offspring produced over a series of ten 2-d intervals were recorded. From these values, l(x), l(x)m(x), and R(0) values were constructed. A trimodal distribution of oviposition was observed with the pollen supplement. Increased oviposition rates led to higher female offspring production per female and to a four-fold increase in F. fusca net reproduction on pollen-treated onions.

  4. Biochemical genetics of the circadian rhythm in Neurospora crassa: studies on the cel strain

    International Nuclear Information System (INIS)

    Lakin-Thomas, P.L.

    1985-01-01

    In Neurospora crassa, the cel mutation lengthens the period of the circadian rhythm when the medium is supplemented with linoleic acid (18:2). Double mutant strains were constructed between cel and the clock mutants prd-1 and four alleles at the frq locus. It was found that: (1) the effect of 18:2 on cel was blocked by prd-1, i.e., prd-1 is epistatic to cel. (2) cel and frq interact such that the percent increase in the period produced by 18:2 was inversely proportional to the period of the frq parent. (3) Data from the literature on period effects in double mutant strains support a multiplicative rather than an additive model. A biochemical interpretation of these interactions is discussed, based on the control of flux through metabolic pathways. Because the cel strain is known to be deficient in the pantothenate derivative normally attached to the fatty acid synthetase (FAS) complex, the possibility that cel may affect other pantothenate-modified proteins was investigated. It was found that in the cel + strain, five proteins of molecular weights (M/sub r/) 9000, 19,000, 22,000, 140,000, and 200,000 were labelled with [ 14 C]pantothenate. In the cel strain, only the 200 k (FAS) label was reduced in amount. Therefore, there is no evidence that cel affects circadian rhythmicity through any deficiency other than FAS. A biochemical model for circadian rhythmicity in Neurospora is presented. Oscillations in cytoplasmic and mitochondrial Ca 2+ are proposed; clock mutations are postulated to affect Ca 2+ transporters and the mitochondrial membrane; and phase-shifting effects are accounted for by changes in Ca 2+ or ATP levels

  5. Engineering of family-5 glycoside hydrolase (Cel5A from an uncultured bacterium for efficient hydrolysis of cellulosic substrates.

    Directory of Open Access Journals (Sweden)

    Amar A Telke

    Full Text Available Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellulose-binding module (CBM fusion approaches were successfully applied to obtain properties required for cellulose hydrolysis. After two rounds of error-prone PCR and screening of 3,000 mutants, amino acid substitutions were identified at various positions in thermotolerant mutants. The most heat-tolerant mutant, Cel5A_2R2, showed a 7-fold increase in thermostability. To enhance the affinity and hydrolytic activity of Cel5A on cellulose substrates, the family-6 CBM from Saccharophagus degradans was fused to the C-terminus of the Cel5A_2R2 mutant using overlap PCR. The Cel5A_2R2-CBM6 fusion protein showed 7-fold higher activity than the native Cel5A on Avicel and filter paper. Cellobiose was a major product obtained from the hydrolysis of cellulosic substrates by the fusion enzyme, which was identified by using thin layer chromatography analysis.

  6. Doença celíaca é super-representada em pacientes com constipação

    Directory of Open Access Journals (Sweden)

    Rolf A. A. Pelleboer

    2012-04-01

    Full Text Available OBJETIVO: Tem sido sugerido que pacientes com constipação sejam triados para doença celíaca. Da mesma forma, recomenda-se a investigação desses pacientes para hipotiroidismo e hipercalcemia. Contudo, nenhuma evidência para essas recomendações está disponível até o momento. Assim, propusemos-nos determinar a prevalência de doença celíaca, hipotiroidismo e hipercalcemia em crianças com constipação. MÉTODOS: Estudo de coorte prospectivo com 370 pacientes consecutivos que preencheram os critérios de Roma III para constipação. Esses pacientes foram encaminhados por um clínico geral a um pediatra devido ao fracasso no tratamento com laxantes. RESULTADOS: A biópsia comprovou doença celíaca em sete desses pacientes. Isso é significativamente mais alto (p < 0,001 do que a prevalência de 1:198 de doença celíaca nos Países Baixos. Dois pacientes tinham tiroidite autoimune. Nenhum paciente tinha hipercalcemia. CONCLUSÕES: Conclui-se que a doença celíaca é significativamente super-representada em pacientes com constipação encaminhados por um clínico geral a um pediatra devido ao fracasso no tratamento com laxantes. Todos esses pacientes devem, portanto, ser triados para doença celíaca.

  7. Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution.

    Science.gov (United States)

    Goedegebuur, Frits; Dankmeyer, Lydia; Gualfetti, Peter; Karkehabadi, Saeid; Hansson, Henrik; Jana, Suvamay; Huynh, Vicky; Kelemen, Bradley R; Kruithof, Paulien; Larenas, Edmund A; Teunissen, Pauline J M; Ståhlberg, Jerry; Payne, Christina M; Mitchinson, Colin; Sandgren, Mats

    2017-10-20

    Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal stability of Cel7A limits its use to processes where temperatures are no higher than 50 °C. Enhanced thermal stability is desirable to enable the use of higher processing temperatures and to improve the economic feasibility of industrial biomass conversion. Here, we enhanced the thermal stability of Cel7A through directed evolution. Sites with increased thermal stability properties were combined, and a Cel7A variant (FCA398) was obtained, which exhibited a 10.4 °C increase in T m and a 44-fold greater half-life compared with the wild-type enzyme. This Cel7A variant contains 18 mutated sites and is active under application conditions up to at least 75 °C. The X-ray crystal structure of the catalytic domain was determined at 2.1 Å resolution and showed that the effects of the mutations are local and do not introduce major backbone conformational changes. Molecular dynamics simulations revealed that the catalytic domain of wild-type Cel7A and the FCA398 variant exhibit similar behavior at 300 K, whereas at elevated temperature (475 and 525 K), the FCA398 variant fluctuates less and maintains more native contacts over time. Combining the structural and dynamic investigations, rationales were developed for the stabilizing effect at many of the mutated sites. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Aves explorando flores de Erythrina fusca (Leguminosae, Fabaceae durante a estação seca no Pantanal de Mato Grosso Exploitation of Erythrina fusca (Leguminosae, Fabaceae flowers by birds during the dry season in Pantanal of Mato Grosso, Brazil

    Directory of Open Access Journals (Sweden)

    Ricardo Parrini

    2010-06-01

    Full Text Available Estudos recentes têm evidenciado a importância de flores como recurso alimentar de diversas espécies de aves neotropicais. Este estudo teve o objetivo de listar espécies e descrever o comportamento das aves que se alimentaram de recursos florais de Erythrina fusca (Fabaceae no Pantanal Mato-Grossense. Para isso, foram acompanhadas aves que se alimentaram em 14 árvores floridas dessa espécie em um total de cerca de 25 horas de observação. Como resultados, 20 espécies de aves pertencentes a sete famílias taxonômicas foram observadas alimentando-se de recursos florais de E. fusca. Apesar de várias espécies de aves terem apresentado comportamentos destrutivos em maior proporção, algumas espécies, sobretudo das famílias Trochilidae e Icteridae, apresentaram estratégias alimentares compatíveis com o transporte de pólen. O cruzamento dos dados obtidos neste estudo com os da literatura indicam que a família Icteridae pode ter relevante papel na polinização dessa espécie vegetal.Recent studies reported the importance of flowers in the diet of many Neotropical bird species. In this paper we identify and describe the feeding behaviour of bird species that feed on flower resources of Erythrina fusca (Fabaceae in the Pantanal Mato-Grossense, Brazil. We recorded birds foraging in 14 flowered individuals of E. fusca during 25 hours of observation. We recorded 20 bird species belonging to seven families feeding on the flowers. Several of the observed behaviours were destructive, despite some species, especially the Trochilidae and Icteridae, showed feeding strategies that preserve the integrity of the flowers and are compatible with pollen transportation. The comparison of the data gathered in this study with the available literature indicates that the Icteridae may play an important role in pollination of this tree species.

  9. Mutations in the VNTR of the carboxyl-ester lipase gene (CEL) are a rare cause of monogenic diabetes.

    Science.gov (United States)

    Torsvik, Janniche; Johansson, Stefan; Johansen, Anders; Ek, Jakob; Minton, Jayne; Raeder, Helge; Ellard, Sian; Hattersley, Andrew; Pedersen, Oluf; Hansen, Torben; Molven, Anders; Njølstad, Pål R

    2010-01-01

    We have previously shown that heterozygous single-base deletions in the carboxyl-ester lipase (CEL) gene cause exocrine and endocrine pancreatic dysfunction in two multigenerational families. These deletions were found in the first and fourth repeats of a variable number of tandem repeats (VNTR), which has proven challenging to sequence due to high GC-content and considerable length variation. We have therefore developed a screening method consisting of a multiplex PCR followed by fragment analysis. The method detected putative disease-causing insertions and deletions in the proximal repeats of the VNTR, and determined the VNTR-length of each allele. When blindly testing 56 members of the two families with known single-base deletions in the CEL VNTR, the method correctly assessed the mutation carriers. Screening of 241 probands from suspected maturity-onset diabetes of the young (MODY) families negative for mutations in known MODY genes (95 individuals from Denmark and 146 individuals from UK) revealed no deletions in the proximal repeats of the CEL VNTR. However, we found one Danish patient with a short, novel CEL allele containing only three VNTR repeats (normal range 7-23 in healthy controls). This allele co-segregated with diabetes or impaired glucose tolerance in the patient's family as six of seven mutation carriers were affected. We also identified individuals who had three copies of a complete CEL VNTR. In conclusion, the CEL gene is highly polymorphic, but mutations in CEL are likely to be a rare cause of monogenic diabetes.

  10. Responses of the tropical gorgonian coral Eunicea fusca to ocean acidification conditions

    Science.gov (United States)

    Gómez, C. E.; Paul, V. J.; Ritson-Williams, R.; Muehllehner, N.; Langdon, C.; Sánchez, J. A.

    2015-06-01

    Ocean acidification can have negative repercussions from the organism to ecosystem levels. Octocorals deposit high-magnesium calcite in their skeletons, and according to different models, they could be more susceptible to the depletion of carbonate ions than either calcite or aragonite-depositing organisms. This study investigated the response of the gorgonian coral Eunicea fusca to a range of CO2 concentrations from 285 to 4,568 ppm (pH range 8.1-7.1) over a 4-week period. Gorgonian growth and calcification were measured at each level of CO2 as linear extension rate and percent change in buoyant weight and calcein incorporation in individual sclerites, respectively. There was a significant negative relationship for calcification and CO2 concentration that was well explained by a linear model regression analysis for both buoyant weight and calcein staining. In general, growth and calcification did not stop in any of the concentrations of pCO2; however, some of the octocoral fragments experienced negative calcification at undersaturated levels of calcium carbonate (>4,500 ppm) suggesting possible dissolution effects. These results highlight the susceptibility of the gorgonian coral E. fusca to elevated levels of carbon dioxide but suggest that E. fusca could still survive well in mid-term ocean acidification conditions expected by the end of this century, which provides important information on the effects of ocean acidification on the dynamics of coral reef communities. Gorgonian corals can be expected to diversify and thrive in the Atlantic-Eastern Pacific; as scleractinian corals decline, it is likely to expect a shift in these reef communities from scleractinian coral dominated to octocoral/soft coral dominated under a "business as usual" scenario of CO2 emissions.

  11. Response to Comment on "Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA".

    Science.gov (United States)

    Brunecky, Roman; Alahuhta, Markus; Xu, Qi; Donohoe, Bryon S; Crowley, Michael F; Kataeva, Irina A; Yang, Sung-Jae; Resch, Michael G; Adams, Michael W W; Lunin, Vladimir V; Himmel, Michael E; Bomble, Yannick J

    2014-05-09

    Gusakov critiques our methodology for comparing the cellulolytic activity of the bacterial cellulase CelA with the fungal cellulase Cel7A. We address his concerns by clarifying some misconceptions, carefully referencing the literature, and justifying our approach to point out that the results from our study still stand.

  12. Elucidating the formation of terra fuscas using Sr–Nd–Pb isotopes and rare earth elements

    International Nuclear Information System (INIS)

    Hissler, Christophe; Stille, Peter; Juilleret, Jérôme; Iffly, Jean François; Perrone, Thierry; Morvan, Gilles

    2015-01-01

    Highlights: • Geochemical evidences on stabile phase confirm Bajocian marl as terra fusca parent material. • Precipitation/Dissolution of secondary carbonates controls geochemistry of labile phases. • This terra fusca sequence record at least four geological and environmental events. - Abstract: Carbonate weathering mantles, like terra fusca, are common in Europe but their formation and evolution is still badly understood. We propose to combine geological, mineralogical and pedological knowledge with trace element and isotope data of a weathering mantle as a novel approach to understand the evolution of terra fuscas. Sr–Nd–Pb isotopes and rare earth element (REE) contents were analyzed in a cambisol developing on a typical terra fusca on top of a condensed Bajocian limestone-marl succession from the eastern side of the Paris Basin. The isotope data, REE distribution patterns and mass balance calculations suggest that the cambisol mirrors the trace element enrichments present in this carbonate lithology, which are exceptionally high compared to global average carbonate. The deeper soil horizons are strongly enriched not only in REE (ΣREE: 2640 ppm) but also in redox-sensitive elements such as Fe (44 wt.%), V (1000 ppm), Cr (700 ppm), Zn (550 ppm), As (260 ppm), Co (45 ppm) and Cd (2.4 ppm). The trace element distribution patterns of the carbonate bedrock are similar to those of the soil suggesting their close genetic relationships. Sr–Nd–Pb isotope data allow to identify four principal components in the soil: a silicate-rich pool close to the surface, a leachable REE enriched pool at the bottom of the soil profile, the limestone on which the weathering profile developed and an anthropogenic, atmosphere-derived component detected in the soil leachates of the uppermost soil horizon. The leachable phases are mainly secondary carbonate-bearing REE phases such as bastnaesite ((X) Ca(CO 3 ) 2 F) (for X: Ce, La and Nd). The isotope data and trace element

  13. Karyological observations on Turbellaria Proseriata: karyometric analysis of Monocelis fusca, M. lineata (Monocelididae) and Parotoplana macrostyla (Otoplanidae)

    Science.gov (United States)

    Curini-Galletti, M.; Galleni, L.; Puccinelli, I.

    1984-03-01

    A karyometric analysis of the chromosome set of the marine turbellarians Monocelis fusca, M. lineata and Parotoplana macrostyla has been carried out. The karyotype of the two Monocelis species investigated (2n=6) is formed by three pairs of small and similarly sized chromosomes: In M. fusca, chromosome 1 is metacentric, chromosome 2 acrocentric and chromosome 3 is subtelocentric. M. lineata also presents one pair of metacentric chromosomes (chromosome 2), while chromosomes 1 and 3 are submetacentric. P. macrostyla (2n=12) reveals two pairs of large metacentric and four pairs of small chromosomes, three of which are metacentric, whereas the last is subtelocentric.

  14. Ecology of the African Maize Stalk Borer, Busseola fusca (Lepidoptera: Noctuidae with Special Reference to Insect-Plant Interactions

    Directory of Open Access Journals (Sweden)

    Paul-André Calatayud

    2014-07-01

    Full Text Available Busseola fusca (Lepidoptera: Noctuidae is an important pest of maize and sorghum in sub-Saharan Africa. One century after its first description by Fuller in 1901, inaccurate information based on earlier reports are still propagated on its distribution (e.g., absent from the lower altitudes in East Africa and host plant range (e.g., feeding on a large range of wild grass species. This review provides updated information on the biology, distribution and genetics of B. fusca with emphasis on insect-plant interactions. Related to this, new avenues of stem borer management are proposed.

  15. Doença celíaca: avaliação da obediência à dieta isenta de glúten e do conhecimento da doença pelos pacientes cadastrados na Associação dos Celíacos do Brasil (ACELBRA Celiac disease: evaluation of compliance to a gluten-free diet and knowledge of the disease in celiac patients registered at the Brazilian Celiac Association (BCA

    Directory of Open Access Journals (Sweden)

    Vera Lucia SDEPANIAN

    2001-10-01

    Full Text Available Racional — A obediência à dieta isenta de glúten previne a ocorrência de complicações malignas e não-malignas. Objetivo - Avaliar a obediência à dieta isenta de glúten e o conhecimento teórico acerca da doença celíaca e seu tratamento pelos pacientes cadastrados na Associação dos Celíacos do Brasil (ACELBRA. Métodos - Foi enviado por correio um questionário a respeito da obediência à dieta isenta de glúten e do conhecimento teórico da doença celíaca e seu tratamento a 584 membros cadastrados na ACELBRA. Resultados - Dos 534 (91,4% questionários recebidos, foram analisados 529 (90,6%. Quanto à obediência à dieta, 69,4% dos pacientes responderam que nunca ingerem glúten e 29,5% que não obedecem à dieta. A proporção de pacientes que ingerem glúten freqüentemente ou sem restrição alguma é maior entre aqueles com idade igual ou maior a 21 anos (17,7% do que os com idade menor (9,9%. A freqüência de obediência à dieta foi maior quando o intervalo de tempo em que foi estabelecido o diagnóstico da doença foi inferior a 5 anos. O intestino delgado foi assinalado como o principal órgão afetado na doença celíaca por 82% dos pacientes. Os principais sintomas assinalados foram diarréia (96,6%, emagrecimento (93,4%, barriga inchada (90,4%, anemia (68,1% e vômitos (59,6%. Apenas 59,0% concordaram com a existência de predisposição genética. Segundo 90,4% das respostas, a doença é permanente e 96,2% assinalaram que a dieta deve ser totalmente isenta de glúten; 67,1% responderam que o glúten é uma proteína que está presente, segundo 92,1% dos inquéritos, no trigo, centeio, cevada e aveia. Observou-se maior proporção de obediência à dieta quando há conhecimento da doença e dieta. A biopsia de intestino delgado foi considerada necessária por apenas 67,5% dos pacientes, observando-se maior freqüência de obediência à dieta nos pacientes que realizaram pelo menos uma biopsia de intestino delgado

  16. Inter-domain Synergism Is Required for Efficient Feeding of Cellulose Chain into Active Site of Cellobiohydrolase Cel7A*

    OpenAIRE

    Kont, Riin; Kari, Jeppe; Borch, Kim; Westh, Peter; Väljamäe, Priit

    2016-01-01

    Structural polysaccharides like cellulose and chitin are abundant and their enzymatic degradation to soluble sugars is an important route in green chemistry. Processive glycoside hydrolases (GHs), like cellobiohydrolase Cel7A of Trichoderma reesei (TrCel7A) are key components of efficient enzyme systems. TrCel7A consists of a catalytic domain (CD) and a smaller carbohydrate-binding module (CBM) connected through the glycosylated linker peptide. A tunnel-shaped active site rests in the CD and ...

  17. CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

    Directory of Open Access Journals (Sweden)

    Tamar Hashimshony

    2012-09-01

    Full Text Available High-throughput sequencing has allowed for unprecedented detail in gene expression analyses, yet its efficient application to single cells is challenged by the small starting amounts of RNA. We have developed CEL-Seq, a method for overcoming this limitation by barcoding and pooling samples before linearly amplifying mRNA with the use of one round of in vitro transcription. We show that CEL-Seq gives more reproducible, linear, and sensitive results than a PCR-based amplification method. We demonstrate the power of this method by studying early C. elegans embryonic development at single-cell resolution. Differential distribution of transcripts between sister cells is seen as early as the two-cell stage embryo, and zygotic expression in the somatic cell lineages is enriched for transcription factors. The robust transcriptome quantifications enabled by CEL-Seq will be useful for transcriptomic analyses of complex tissues containing populations of diverse cell types.

  18. Acid hydrolysis of kallar grass (leptochloa fusca) for the production

    International Nuclear Information System (INIS)

    Chughtai, F.A.; Shah, M.H.

    1993-01-01

    Acid hydrolysis of kallar grass (leptochloa fusca) was carried of with various concentrations of sulphuric acid, ortho phosphoric acid and hydrochloric acid to produce furfural. The study revealed that activity of various hydrolysing acids to produce furfural from kallar grass was of the following order H/sub 2/SO/sub 4/ > H/sub 3/PO/sub 4/ > HCl. Optimum yield (4.78%) of the produce was obtained when the material was digested with 19% H/sub 2/SO/sub 4/ for a period of 20 minutes. (author)

  19. VIDEO ANIMASI 3D PENGENALAN RUMAH ADAT DAN ALAT MUSIK KEPRI DENGAN MENGUNAKAN TEKNIK RENDER CEL-SHADING

    Directory of Open Access Journals (Sweden)

    Jianfranco Irfian Asnawi

    2016-11-01

    Full Text Available Animasi ini berjudul "video animasi 3D rumah adat dan alat musik Kepulauan Riau dengan menggunakan teknik render cel-shading" merupakan video yang bertujuan memperkenalkan alat-alat musik yang berasal dari kepulauan riau, Animasi ini akan diterapkan dengan menggunakan teknik render cel-shading. Cel-shading adalah teknik render yang menampilkan grafik 3D yang menyerupai gambar tangan, seperti gambar komik dan kartun. Teknik ini juga sudah di terapkan dalam game 3D yang ternyata menarik banyak perhatian peminat. Teknik ini akan di terapkan kedalam animasi 3D "video animasi rumah adat dan alat musik kepulauan riau dengan menggunakan teknik render cel-shading" Animasi di rancang menggunakan skenario dan storyboard kemudian di implementasikan dalam software 3D MAYA AUTODESK dengan menggunakan teknik render cel-shading. Setelah diterapkan maka di dapatkan definisi keberhasilan dari teknik render cel shading di bandingkan dengan teknik render global illumination seperti dari kecepatan dalam merender dan tingkat kecerahan warna pada video. Kata kunci: animasi, game 3D, cel-shading.

  20. Inter-domain synergism is required for efficient feeding of cellulose chain into active site of cellobiohydrolase Cel7A

    DEFF Research Database (Denmark)

    Kont, Riin; Kari, Jeppe; Borch, Kim

    2016-01-01

    systems. TrCel7A consists of catalytic domain (CD) and a smaller carbohydrate binding module (CBM) connected through the glycosylated linker peptide. A tunnel shaped active site rests in the CD and contains 10 glucose unit binding sites. The active site of TrCel7A is lined with four Trp residues with two...... to Ala substitution on on-rates was strongly dependent on the presence of the CBM-linker. This compensation between CBM-linker and Trp-38 indicates synergism between CBM-linker and CD in feeding the cellulose chain into the active site. The inter-domain synergism was pre-requisite for the efficient......Structural polysaccharides like cellulose and chitin are abundant and their enzymatic degradation to soluble sugars is an important route in green chemistry. Processive glycoside hydrolases (GHs), like cellobiohydrolase Cel7A of Trichoderma reesei (TrCel7A) are key components of efficient enzyme...

  1. Formation of elemental sulfur by Chlorella fusca during growth on L-cysteine ethylester

    Energy Technology Data Exchange (ETDEWEB)

    Krauss, F.; Schafer, W.; Schmidt, A.

    1984-01-01

    During growth on L-cysteine ethylester, Chlorella fusca (211-8b) accumulated a substance which contained bound sulfide, which could be liberated by reduction with dithioerythritol (DTE) as inorganic sulfide. This substance was extracted with hot methanol and purified by thin layer chromatography. This substance liberated free sulfide when incubated with mono- and dithiols, and thiocyanate was formed after heating with KCN. The isolated substance cochromatographed with authentic sulfur flower using different solvent systems for thin layer chromatography, high pressure liquid chromatography, and the identical spectrum with a relative ..beta..max at 263 nm was found. The chemical structure was confirmed by mass spectrometry showing a molecular weight of 256 m/e for the S/sub 8/ configuration. No labeled elemental sulfur was detected when the cells were grown on (/sup 35/S)sulfate and L-cysteine ethylester. C. fusca seems to have enzymes for the metabolism of elemental sulfur, since it disappeared after prolonged growth into the stationary phase. Cysteine was formed from O-acetyl-L-serine and elemental sulfur in the presence of thiol groups and purified cysteine synthase from spinach or Chlorella.

  2. Use of mep HyperCel for polishing of human serum albumin.

    Science.gov (United States)

    McCann, Karl B; Vucica, Yvonne; Wu, John; Bertolini, Joseph

    2014-10-15

    The manufacture of human serum albumin by chromatographic procedures involves gel filtration chromatography as a final polishing step. Despite this step being essential to remove high molecular weight impurity proteins and thus ensure a stable and safe final product, it is relatively inefficient. This paper explores the use of hydrophobic charge induction chromatographic media, MEP HyperCel as an alternative to Sephacryl S200HR gel filtration for the polishing of human serum albumin derived by ion exchange chromatographic purification of Cohn Supernatant I. The use of MEP HyperCel results in a product with a higher purity than achieved with gel filtration and in a less time consuming manner and with potential resource savings. MEP HyperCel appears to have great potential for incorporation into downstream processes in the plasma fractionation industry as an efficient means of achieving polishing of intermediates or capture of proteins of interest. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. 9 CFR 3.90 - Care in transit.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Care in transit. 3.90 Section 3.90 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE STANDARDS Specifications for the Humane Handling, Care, Treatment, and Transportation of Nonhuman...

  4. Hydrodynamics and photosynthesis performance of Chlorella fusca (Chlorophyta) grown in a thin-layer cascade (TLC) system

    Czech Academy of Sciences Publication Activity Database

    Jerez, Celia G.; Navarro, E.; Rico, Rosa M.; Malpartida, I.; Masojídek, Jiří; Abdala, R.; Figueroa, Félix L.

    2014-01-01

    Roč. 22, č. 2 (2014), s. 111-122 ISSN 1864-7790 R&D Projects: GA MŠk ED2.1.00/03.0110 Grant - others:Government of Spain (ES) Project Ecolife CGL08-05407-C03-01; Junta de Andalucía(ES) RNM-295; Ministry of Economy and Competitiveness(ES) CTM2011-15659-E Institutional support: RVO:61388971 Keywords : Chlorella fusca * TLC * cultivation * photosynthesis Subject RIV: EE - Microbiology, Virology Impact factor: 1.258, year: 2014

  5. Single-molecule Imaging Analysis of Elementary Reaction Steps of Trichoderma reesei Cellobiohydrolase I (Cel7A) Hydrolyzing Crystalline Cellulose Iα and IIII*

    Science.gov (United States)

    Shibafuji, Yusuke; Nakamura, Akihiko; Uchihashi, Takayuki; Sugimoto, Naohisa; Fukuda, Shingo; Watanabe, Hiroki; Samejima, Masahiro; Ando, Toshio; Noji, Hiroyuki; Koivula, Anu; Igarashi, Kiyohiko; Iino, Ryota

    2014-01-01

    Trichoderma reesei cellobiohydrolase I (TrCel7A) is a molecular motor that directly hydrolyzes crystalline celluloses into water-soluble cellobioses. It has recently drawn attention as a tool that could be used to convert cellulosic materials into biofuel. However, detailed mechanisms of action, including elementary reaction steps such as binding, processive hydrolysis, and dissociation, have not been thoroughly explored because of the inherent challenges associated with monitoring reactions occurring at the solid/liquid interface. The crystalline cellulose Iα and IIII were previously reported as substrates with different crystalline forms and different susceptibilities to hydrolysis by TrCel7A. In this study, we observed that different susceptibilities of cellulose Iα and IIII are highly dependent on enzyme concentration, and at nanomolar enzyme concentration, TrCel7A shows similar rates of hydrolysis against cellulose Iα and IIII. Using single-molecule fluorescence microscopy and high speed atomic force microscopy, we also determined kinetic constants of the elementary reaction steps for TrCel7A against cellulose Iα and IIII. These measurements were performed at picomolar enzyme concentration in which density of TrCel7A on crystalline cellulose was very low. Under this condition, TrCel7A displayed similar binding and dissociation rate constants for cellulose Iα and IIII and similar fractions of productive binding on cellulose Iα and IIII. Furthermore, once productively bound, TrCel7A processively hydrolyzes and moves along cellulose Iα and IIII with similar translational rates. With structural models of cellulose Iα and IIII, we propose that different susceptibilities at high TrCel7A concentration arise from surface properties of substrate, including ratio of hydrophobic surface and number of available lanes. PMID:24692563

  6. Copy number variants and VNTR length polymorphisms of the carboxyl-ester lipase (CEL) gene as risk factors in pancreatic cancer.

    Science.gov (United States)

    Dalva, Monica; El Jellas, Khadija; Steine, Solrun J; Johansson, Bente B; Ringdal, Monika; Torsvik, Janniche; Immervoll, Heike; Hoem, Dag; Laemmerhirt, Felix; Simon, Peter; Lerch, Markus M; Johansson, Stefan; Njølstad, Pål R; Weiss, Frank U; Fjeld, Karianne; Molven, Anders

    We have recently described copy number variants (CNVs) of the human carboxyl-ester lipase (CEL) gene, including a recombined deletion allele (CEL-HYB) that is a genetic risk factor for chronic pancreatitis. Associations with pancreatic disease have also been reported for the variable number of tandem repeat (VNTR) region located in CEL exon 11. Here, we examined if CEL CNVs and VNTR length polymorphisms affect the risk for developing pancreatic cancer. CEL CNVs and VNTR were genotyped in a German family with non-alcoholic chronic pancreatitis and pancreatic cancer, in 265 German and 197 Norwegian patients diagnosed with pancreatic adenocarcinoma, and in 882 controls. CNV screening was performed using PCR assays followed by agarose gel electrophoresis whereas VNTR lengths were determined by DNA fragment analysis. The investigated family was CEL-HYB-positive. However, an association of CEL-HYB or a duplication CEL allele with pancreatic cancer was not seen in our two patient cohorts. The frequency of the 23-repeat VNTR allele was borderline significant in Norwegian cases compared to controls (1.2% vs. 0.3%; P = 0.05). For all other VNTR lengths, no statistically significant difference in frequency was observed. Moreover, no association with pancreatic cancer was detected when CEL VNTR lengths were pooled into groups of short, normal or long alleles. We could not demonstrate an association between CEL CNVs and pancreatic cancer. An association is also unlikely for CEL VNTR lengths, although analyses in larger materials are necessary to completely exclude an effect of rare VNTR alleles. Copyright © 2016 IAP and EPC. Published by Elsevier B.V. All rights reserved.

  7. La Enfermedad Celíaca

    OpenAIRE

    Cueto Rua, Eduardo Ángel; Nanfito, Gabriela; Guzmán, Luciana

    2006-01-01

    La Enfermedad Celíaca (EC) es la intolerancia alimentaria de orden genético más frecuente de la especie humana. En nuestro Servicio de Gastroenterología hemos diagnosticado más de 1900 casos en los últimos 34 años, y 92 y 73 nuevos casos en los años 2004 y 2005, respectivamente. La EC es el resultado de la interacción entre factores genéticos (constante absoluta) expresados en la mucosa intestinal y la respuesta i...

  8. Cuticular chemistry of males and females in the ant Formica fusca

    DEFF Research Database (Denmark)

    Chernenko, Anton; Holman, Luke; Helanterä, Heikki

    2012-01-01

    in mediating recognition of society members and, thereby, allowing kin selection to operate. Here, we examined whether cuticular hydrocarbon profiles are sex-specific and whether males and young queens of the ant Formica fusca have colony-specific profiles. We also investigated whether there is a relationship...... between genetic relatedness and chemical diversity within colonies. We demonstrated that female and male sexuals do not have unique sex-specific compounds, but that there are quantitative chemical differences between the sexes. Out of the 51 cuticular hydrocarbon compounds identified, 10 showed...

  9. Loop variants of the thermophile Rasamsonia emersonii Cel7A with improved activity against cellulose

    DEFF Research Database (Denmark)

    Sørensen, Trine Holst; Skovbo Windahl, Michael; McBrayer, Brett

    2017-01-01

    Cel7A cellobiohydrolases perform processive hydrolysis on one strand of cellulose, which is threaded through the enzyme's substrate binding tunnel. The tunnel structure results from a groove in the catalytic domain, which is covered by a number of loops. These loops have been identified as potent......Cel7A cellobiohydrolases perform processive hydrolysis on one strand of cellulose, which is threaded through the enzyme's substrate binding tunnel. The tunnel structure results from a groove in the catalytic domain, which is covered by a number of loops. These loops have been identified...... as potential targets for engineering of this industrially important enzyme family, but only few systematic studies on this have been made. Here we show that two asparagine residues (N194 and N197) positioned in the loop covering the glucopyranose subsite −4 (recently denoted B2 loop) of the thermostable Cel7A...... with alanine leads to faster enzyme-substrate dissociation. Conversely, these residues appeared to have little or no effect on the rate of association. We suggest that the controlled adjustment of the enzyme-substrate dissociation prompts faster cellulolytic enzymes....

  10. IMPACTS OF BIOFILM FORMATION ON CELLULOSE FERMENTATION

    Energy Technology Data Exchange (ETDEWEB)

    Leschine, Susan

    2009-10-31

    This project addressed four major areas of investigation: i) characterization of formation of Cellulomonas uda biofilms on cellulose; ii) characterization of Clostridium phytofermentans biofilm development; colonization of cellulose and its regulation; iii) characterization of Thermobifida fusca biofilm development; colonization of cellulose and its regulation; and iii) description of the architecture of mature C. uda, C. phytofermentans, and T. fusca biofilms. This research is aimed at advancing understanding of biofilm formation and other complex processes involved in the degradation of the abundant cellulosic biomass, and the biology of the microbes involved. Information obtained from these studies is invaluable in the development of practical applications, such as the single-step bioconversion of cellulose-containing residues to fuels and other bioproducts. Our results have clearly shown that cellulose-decomposing microbes rapidly colonize cellulose and form complex structures typical of biofilms. Furthermore, our observations suggest that, as cells multiply on nutritive surfaces during biofilms formation, dramatic cell morphological changes occur. We speculated that morphological changes, which involve a transition from rod-shaped cells to more rounded forms, might be more apparent in a filamentous microbe. In order to test this hypothesis, we included in our research a study of biofilm formation by T. fusca, a thermophilic cellulolytic actinomycete commonly found in compost. The cellulase system of T. fusca has been extensively detailed through the work of David Wilson and colleagues at Cornell, and also, genome sequence of a T. fusca strain has been determine by the DOE Joint Genome Institute. Thus, T. fusca is an excellent subject for studies of biofilm development and its potential impacts on cellulose degradation. We also completed a study of the chitinase system of C. uda. This work provided essential background information for understanding how C. uda

  11. The impact of a parasitic nematode Thripinema fuscum (Tylenchida: Allantonematidae) on the feeding behavior and vector competence of Frankliniella fusca (Thysanoptera: Thripidae)

    Science.gov (United States)

    Frankliniella fusca (Hinds) (Thysanoptera: Thripidae) is the predominant thrips species found inhabiting and reproducing in peanut (Arachis hypogaea L.) and is one of at least seven thrips species reported to transmit Tomato spotted wilt virus (TSWV). The entomogenous nematode Thripinema fuscum Tipp...

  12. Inter-domain Synergism Is Required for Efficient Feeding of Cellulose Chain into Active Site of Cellobiohydrolase Cel7A.

    Science.gov (United States)

    Kont, Riin; Kari, Jeppe; Borch, Kim; Westh, Peter; Väljamäe, Priit

    2016-12-09

    Structural polysaccharides like cellulose and chitin are abundant and their enzymatic degradation to soluble sugars is an important route in green chemistry. Processive glycoside hydrolases (GHs), like cellobiohydrolase Cel7A of Trichoderma reesei (TrCel7A) are key components of efficient enzyme systems. TrCel7A consists of a catalytic domain (CD) and a smaller carbohydrate-binding module (CBM) connected through the glycosylated linker peptide. A tunnel-shaped active site rests in the CD and contains 10 glucose unit binding sites. The active site of TrCel7A is lined with four Trp residues with two of them, Trp-40 and Trp-38, in the substrate binding sites near the tunnel entrance. Although addressed in numerous studies the elucidation of the role of CBM and active site aromatics has been obscured by a complex multistep mechanism of processive GHs. Here we studied the role of the CBM-linker and Trp-38 of TrCel7A with respect to binding affinity, on- and off-rates, processivity, and synergism with endoglucanase. The CBM-linker increased the on-rate and substrate affinity of the enzyme. The Trp-38 to Ala substitution resulted in increased off-rates and decreased processivity. The effect of the Trp-38 to Ala substitution on on-rates was strongly dependent on the presence of the CBM-linker. This compensation between CBM-linker and Trp-38 indicates synergism between CBM-linker and CD in feeding the cellulose chain into the active site. The inter-domain synergism was pre-requisite for the efficient degradation of cellulose in the presence of endoglucanase. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  13. PERFORMANCE OF LIQUI-CEL EXTRA-FLOW MEMBRANE CONTRACTOR IN A PURE WATER AND IN A 0.2% SODIUM CHLORIDE SOLUTION (SNO-STR-2001-11).

    Energy Technology Data Exchange (ETDEWEB)

    YEH,M.; BOGER,J.; HAHN,R.L.

    2001-11-05

    After completion of SNO's first phase measurement of the neutrino charge current, two tons of salt were added into the SNO heavy water to increase the sensitivity of the neutral current measurement (Phase II). Liqui-Cel Extra-Flow Membrane Contactors (simply called Liqui-Cel) are used in the SNO heavy-water circulating system to remove the dissolved gases, such as oxygen, nitrogen, radon, and water vapor from the liquid water. One possible scenario with phase II operation is that the salt may leak through the Liqui-Cel Membrane and come in contact with the vacuum pumps and other metal components of the Heavy-Water Vapor Recovery System. In this scenario, corrosion will damage these components, especially the vacuum pump (Pfeiffer UniDry Pump with cast iron interior), and increase the operational difficulties. A series of tests for the behavior of the Liqui-Cel System in pure water and in salt systems was conducted at the Brookhaven National Laboratory in order to measure the transfer of (a) water vapor and (b) salt, if there is any, through the membrane. Initially a 10-inch by 28-inch Liqui-Cel unit, identical to those used in the SNO heavy-water circulating system, was obtained from SNO site. However, extensive analysis showed that the membrane in this unit was defective: a replacement membrane would cost several thousand dollars. Instead, a smaller, 2.5-inch x 8-inch Liqui-Cel, obtained from Dr. Richard Helmers of the University of British Columbia, was used in this experiment. A comparison of the present experiment with the SNO heavy-water system is done with theoretical calculations. The results are discussed in the following sections.

  14. VIDEO ANIMASI 3D PENGENALAN RUMAH ADAT DAN ALAT MUSIK KEPRI DENGAN MENGUNAKAN TEKNIK RENDER CEL-SHADING

    OpenAIRE

    Jianfranco Irfian Asnawi; Afdhol Dzikri

    2016-01-01

    Animasi ini berjudul "video animasi 3D rumah adat dan alat musik Kepulauan Riau dengan menggunakan teknik render cel-shading" merupakan video yang bertujuan memperkenalkan alat-alat musik yang berasal dari kepulauan riau, Animasi ini akan diterapkan dengan menggunakan teknik render cel-shading. Cel-shading adalah teknik render yang menampilkan grafik 3D yang menyerupai gambar tangan, seperti gambar komik dan kartun. Teknik ini juga sudah di terapkan dalam game 3D yang ternyata menarik banyak ...

  15. Pesquisa de gliadina em medicamentos: informação relevante para a orientação de pacientes com doença celíaca

    Directory of Open Access Journals (Sweden)

    SDEPANIAN Vera Lucia

    2001-01-01

    Full Text Available Racional - Alguns medicamentos podem conter gliadina, portanto, sua utilização poderá ser prejudicial aos pacientes com doença celíaca. Objetivo - Detectar a presença de gliadina em medicamentos comumente comercializados no Brasil. Métodos - Foram analisados 78 medicamentos sorteados a partir de uma lista de 180 produtos comumente comercializados. Os medicamentos analisados foram: analgésicos (n = 9, anti-helmínticos (n = 3, antiácidos (n = 8, antibióticos (n = 13, anticolesterolêmicos (n = 1, anticonvulsivantes (n = 2, antidepressivos (n = 2, antidiabéticos (n = 1, antieméticos (n = 3, anti-hipertensivos (n = 3, anti-histamínicos (n = 3, antiinflamatórios (n = 7, antitérmicos (n = 2, broncodilatadores (n = 1, descongestionantes (n = 4, laxantes (n = 1, contraceptivos orais (n = 5 e vitaminas (n = 10. As amostras foram analisadas pela técnica de ELISA utilizando anticorpo monoclonal ômega-gliadina, considerada de eleição segundo o Codex Alimentarius Commission WHO/FAO. Todas as amostras foram analisadas em duplicata. O nível de detecção do teste é de 4 mg de gliadina/100 g de produto. Resultados - Dentre os 78 medicamentos analisados, em apenas 1 (1,3% foi detectada a presença de gliadina (5,5 mg/100 g. O componente ativo do medicamento é ranitidina. De acordo com o Codex Alimentarius Commission WHO/FAO, o limite máximo diário permitido ao consumo pelos indivíduos com doença celíaca é igual a 10 mg de gliadina. Considerando a quantidade de gliadina num único comprimido de ranitidina, a quantidade ingerida seria bem menor do que a máxima permitida ao consumo pelo paciente com doença celíaca. Conclusões - Neste estudo não foi identificada gliadina em medicamentos em quantidade que constitua risco para pacientes com doença celíaca.

  16. TAHSĐN YÜCEL'S SHORT STORIES IN HIS BOOK 'MYSELF AND THE OTHER’ READING WITH THE 9th SYMPHONY OR THE CIRCUMSTANCES OF ÖTEGEÇE'S IN THE RHYTM OF 9th SYMPHONY

    Directory of Open Access Journals (Sweden)

    Ferhan AKGÜN (M.A.H.

    2009-01-01

    Full Text Available Literary works transforming in time have started toemerge in especially personal inclinations, psychologicalanalysis and universal matters; and have pushed thelimit lines lately. This limit was not only all about thecontent of the literature but also reflected towards theother fields of art. This intertextuality forms the roots ofhis short stories within “Me and the Others” by TahsinYücel, who is among the short story writers of 1950s.Tahsin Yücel expreses this in his words: “there exists arelation between the smallest and the greatest structures,between my structure and the others’. Reflecting theindividual and his inner world, departing from theindividual and giving messages to the society andhumankind, the writer endeavours to reach from localmatters to universal ones in his short stories within “Meand the Others”.

  17. A novel pH-stable, endoglucanase (JqCel5A isolated from a salt-lake microorganism, Jonesia quinghaiensis

    Directory of Open Access Journals (Sweden)

    Ling Lin

    2016-11-01

    Conclusions: It was believed that these properties might make JqCel5A to be potentially used in the suitable industrial catalytic condition, which has a broad pH fluctuation and/or chemical disturbance.

  18. Aspectos psicosociales de la enfermedad celíaca en España: una vida libre de gluten

    Directory of Open Access Journals (Sweden)

    Julián Rodríguez ALMAGRO

    Full Text Available RESUMEN Objetivo: La celiaquía como enfermedad crónica tiene una alta prevalencia en nuestra sociedad. El artículo analiza los aspectos psicosociales de la enfermedad celíaca en los diferentes entornos, valorando el impacto de la implantación de una dieta estricta libre de gluten. Métodos: Estudio cualitativo entre los meses de Enero del 2013 a Abril del 2013 en el cual, a través de la teoría fundamentada y el análisis de contenido, se ha profundizado en el análisis de las entrevistas semiestructuradas a personas con enfermedad celíaca en España. Se realizaron llamamientos a traves de las redes sociales y grupos de celíacos hasta llegar a la saturación teórica, que determinó el tamaño final de la muestra de 24 personas. Resultados: Educación Sanitaria, Aislamiento social, soledad y desconocimiento social emergieron como categorías centrales en la experiencia de adopción de la dieta libre de gluten a partir del diagnóstico de celiaquía. Conclusión: Los profesionales deben promover y apoyar estrategias de apoyo social basadas en una comprensión íntegra de las experiencias de desarraigo y marginación que los pacientes celíacos experimentan en sus relaciones sociales a través de la comida. Se requiere un aumento de la educación sanitaria para entender e integrar el impacto psicosocial del diagnóstico de celiaquía y la dieta libre de gluten.

  19. Insight into the process of product expulsion in cellobiohydrolase Cel6A from Trichoderma reesei by computational modeling.

    Science.gov (United States)

    Huang, Houhou; Han, Fei; Guan, Shanshan; Qian, Mengdan; Wan, Yongfeng; Shan, Yaming; Zhang, Hao; Wang, Song

    2018-03-24

    Glycoside hydrolase cellulase family 6 from Trichoderma reesei (TrCel6A) is an important cellobiohydrolase to hydrolyze cellooligosaccharide into cellobiose. The knowledge of enzymatic mechanisms is critical for improving the conversion efficiency of cellulose into ethanol or other chemicals. However, the process of product expulsion, a key component of enzymatic depolymerization, from TrCel6A has not yet been described in detail. Here, conventional molecular dynamics and steered molecular dynamics (SMD) were applied to study product expulsion from TrCel6A. Tyr103 may be a crucial residue in product expulsion given that it exhibits two different posthydrolytic conformations. In one conformation, Tyr103 rotates to open the -3 subsite. However, Tyr103 does not rotate in the other conformation. Three different routes for product expulsion were proposed on the basis of the two different conformations. The total energy barriers of the three routes were calculated through SMD simulations. The total energy barrier of product expulsion through Route 1, in which Tyr103 does not rotate, was 22.2 kcal·mol -1 . The total energy barriers of product expulsion through Routes 2 and 3, in which Tyr103 rotates to open the -3 subsite, were 10.3 and 14.4 kcal·mol -1 , respectively. Therefore, Routes 2 and 3 have lower energy barriers than Route 1, and Route 2 is the thermodynamically optimal route for product expulsion. Consequently, the rotation of Tyr103 may be crucial for product release from TrCel6A. Results of this work have potential applications in cellulase engineering.

  20. I.C.F. program at Cel-V

    International Nuclear Information System (INIS)

    Andre, M.; Coutant, J.; Dautray, R.; Decoster, A.; Decroisette, M.; Duborgel, B.; Ouvry, J.; Ovadia, J.; Watteau, J.P.

    1990-01-01

    The principal objective of the CEL-V laser program is to realize high performances ablative implosions. The indirect drive approach has been chosen to reach the high degree of uniformity required. In relation to this objective, the radiation transfer and the development of hydrodynamics instabilities are widely studied. Besides, efforts have been developed in the field of diagnostics, and of laser technology with optical smoothing and high damage level coatings studies

  1. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca

    NARCIS (Netherlands)

    Romero, Diego; de Vicente, Antonio; Rakotoaly, Rivo H.; Dufour, Samuel E.; Veening, Jan-Willem; Arrebola, Eva; Cazorla, Francisco M.; Kuipers, Oscar P.; Paquot, Michel; Perez-Garcia, Alejandro; Stacey, Gary

    Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the

  2. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme.

    Science.gov (United States)

    Pellegrini, Vanessa O A; Serpa, Viviane Isabel; Godoy, Andre S; Camilo, Cesar M; Bernardes, Amanda; Rezende, Camila A; Junior, Nei Pereira; Franco Cairo, João Paulo L; Squina, Fabio M; Polikarpov, Igor

    2015-11-01

    Trichoderma filamentous fungi have been investigated due to their ability to secrete cellulases which find various biotechnological applications such as biomass hydrolysis and cellulosic ethanol production. Previous studies demonstrated that Trichoderma harzianum IOC-3844 has a high degree of cellulolytic activity and potential for biomass hydrolysis. However, enzymatic, biochemical, and structural studies of cellulases from T. harzianum are scarce. This work reports biochemical characterization of the recombinant endoglucanase I from T. harzianum, ThCel7B, and its catalytic core domain. The constructs display optimum activity at 55 °C and a surprisingly acidic pH optimum of 3.0. The full-length enzyme is able to hydrolyze a variety of substrates, with high specific activity: 75 U/mg for β-glucan, 46 U/mg toward xyloglucan, 39 U/mg for lichenan, 26 U/mg for carboxymethyl cellulose, 18 U/mg for 4-nitrophenyl β-D-cellobioside, 16 U/mg for rye arabinoxylan, and 12 U/mg toward xylan. The enzyme also hydrolyzed filter paper, phosphoric acid swollen cellulose, Sigmacell 20, Avicel PH-101, and cellulose, albeit with lower efficiency. The ThCel7B catalytic domain displays similar substrate diversity. Fluorescence-based thermal shift assays showed that thermal stability is highest at pH 5.0. We determined kinetic parameters and analyzed a pattern of oligosaccharide substrates hydrolysis, revealing cellobiose as a final product of C6 degradation. Finally, we visualized effects of ThCel7B on oat spelt using scanning electron microscopy, demonstrating the morphological changes of the substrate during the hydrolysis. The acidic behavior of ThCel7B and its considerable thermostability hold a promise of its industrial applications and other biotechnological uses under extremely acidic conditions.

  3. Complete mitochondrial genome of Porzana fusca and Porzana pusilla and phylogenetic relationship of 16 Rallidae species.

    Science.gov (United States)

    Chen, Peng; Han, Yuqing; Zhu, Chaoying; Gao, Bin; Ruan, Luzhang

    2017-12-01

    The complete mitochondrial genome sequences of Porzana fusca and Porzana pusilla were determined. The two avian species share a high degree of homology in terms of mitochondrial genome organization and gene arrangement. Their corresponding mitochondrial genomes are 16,935 and 16,978 bp and consist of 37 genes and a control region. Their PCGs were both 11,365 bp long and have similar structure. Their tRNA gene sequences could be folded into canonical cloverleaf secondary structure, except for tRNA Ser (AGY) , which lost its "DHU" arm. Based on the concatenated nucleotide sequences of the complete mitochondrial DNA genes of 16 Rallidae species, reconstruction of phylogenetic trees and analysis of the molecular clock of P. fusca and P. pusilla indicated that these species from a sister group, which in turn are sister group to Rallina eurizonoides. The genus Gallirallus is a sister group to genus Lewinia, and these groups in turn are sister groups to genus Porphyrio. Moreover, molecular clock analyses suggested that the basal divergence of Rallidae could be traced back to 40.47 (41.46‒39.45) million years ago (Mya), and the divergence of Porzana occurred approximately 5.80 (15.16‒0.79) Mya.

  4. Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose*

    Science.gov (United States)

    Nakamura, Akihiko; Tasaki, Tomoyuki; Ishiwata, Daiki; Yamamoto, Mayuko; Okuni, Yasuko; Visootsat, Akasit; Maximilien, Morice; Noji, Hiroyuki; Uchiyama, Taku; Samejima, Masahiro; Igarashi, Kiyohiko; Iino, Ryota

    2016-01-01

    Trichoderma reesei Cel6A (TrCel6A) is a cellobiohydrolase that hydrolyzes crystalline cellulose into cellobiose. Here we directly observed the reaction cycle (binding, surface movement, and dissociation) of single-molecule intact TrCel6A, isolated catalytic domain (CD), cellulose-binding module (CBM), and CBM and linker (CBM-linker) on crystalline cellulose Iα. The CBM-linker showed a binding rate constant almost half that of intact TrCel6A, whereas those of the CD and CBM were only one-tenth of intact TrCel6A. These results indicate that the glycosylated linker region largely contributes to initial binding on crystalline cellulose. After binding, all samples showed slow and fast dissociations, likely caused by the two different bound states due to the heterogeneity of cellulose surface. The CBM showed much higher specificity to the high affinity site than to the low affinity site, whereas the CD did not, suggesting that the CBM leads the CD to the hydrophobic surface of crystalline cellulose. On the cellulose surface, intact molecules showed slow processive movements (8.8 ± 5.5 nm/s) and fast diffusional movements (30–40 nm/s), whereas the CBM-Linker, CD, and a catalytically inactive full-length mutant showed only fast diffusional movements. These results suggest that both direct binding and surface diffusion contribute to searching of the hydrolysable point of cellulose chains. The duration time constant for the processive movement was 7.7 s, and processivity was estimated as 68 ± 42. Our results reveal the role of each domain in the elementary steps of the reaction cycle and provide the first direct evidence of the processive movement of TrCel6A on crystalline cellulose. PMID:27609516

  5. Gclust Server: 136363 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 136363 CEL_R04E5.9_17569269 Cluster Sequences - 175 R04E5.9 1 1.00e-90 0.0 0.0 0.0 0.0 0.0 12.5 Show 13636...3 Cluster ID 136363 Sequence ID CEL_R04E5.9_17569269 Link to cluster sequences Cluste

  6. Structural and functional characterizations of SsgB, a conserved activator of developmental cell division in morphologically complex actinomycetes.

    Science.gov (United States)

    Xu, Qingping; Traag, Bjørn A; Willemse, Joost; McMullan, Daniel; Miller, Mitchell D; Elsliger, Marc-André; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Chruszcz, Maksymilian; Clayton, Thomas; Das, Debanu; Deller, Marc C; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L; Feuerhelm, Julie; Grant, Joanna C; Grzechnik, Anna; Grzechnik, Slawomir K; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K; Klock, Heath E; Knuth, Mark W; Kozbial, Piotr; Krishna, S Sri; Kumar, Abhinav; Marciano, David; Minor, Wladek; Mommaas, A Mieke; Morse, Andrew T; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L; Sefcovic, Natasha; Tien, Henry J; Trame, Christine B; van den Bedem, Henry; Wang, Shuren; Weekes, Dana; Hodgson, Keith O; Wooley, John; Deacon, Ashley M; Godzik, Adam; Lesley, Scott A; Wilson, Ian A; van Wezel, Gilles P

    2009-09-11

    SsgA-like proteins (SALPs) are a family of homologous cell division-related proteins that occur exclusively in morphologically complex actinomycetes. We show that SsgB, a subfamily of SALPs, is the archetypal SALP that is functionally conserved in all sporulating actinomycetes. Sporulation-specific cell division of Streptomyces coelicolor ssgB mutants is restored by introduction of distant ssgB orthologues from other actinomycetes. Interestingly, the number of septa (and spores) of the complemented null mutants is dictated by the specific ssgB orthologue that is expressed. The crystal structure of the SsgB from Thermobifida fusca was determined at 2.6 A resolution and represents the first structure for this family. The structure revealed similarities to a class of eukaryotic "whirly" single-stranded DNA/RNA-binding proteins. However, the electro-negative surface of the SALPs suggests that neither SsgB nor any of the other SALPs are likely to interact with nucleotide substrates. Instead, we show that a conserved hydrophobic surface is likely to be important for SALP function and suggest that proteins are the likely binding partners.

  7. Structural and Functional Characterizations of SsgB, a Conserved Activator of Developmental Cell Division in Morphologically Complex Actinomycetes*

    Science.gov (United States)

    Xu, Qingping; Traag, Bjørn A.; Willemse, Joost; McMullan, Daniel; Miller, Mitchell D.; Elsliger, Marc-André; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Chruszcz, Maksymilian; Clayton, Thomas; Das, Debanu; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Anna; Grzechnik, Slawomir K.; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Kumar, Abhinav; Marciano, David; Minor, Wladek; Mommaas, A. Mieke; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; van den Bedem, Henry; Wang, Shuren; Weekes, Dana; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.; van Wezel, Gilles P.

    2009-01-01

    SsgA-like proteins (SALPs) are a family of homologous cell division-related proteins that occur exclusively in morphologically complex actinomycetes. We show that SsgB, a subfamily of SALPs, is the archetypal SALP that is functionally conserved in all sporulating actinomycetes. Sporulation-specific cell division of Streptomyces coelicolor ssgB mutants is restored by introduction of distant ssgB orthologues from other actinomycetes. Interestingly, the number of septa (and spores) of the complemented null mutants is dictated by the specific ssgB orthologue that is expressed. The crystal structure of the SsgB from Thermobifida fusca was determined at 2.6 Å resolution and represents the first structure for this family. The structure revealed similarities to a class of eukaryotic “whirly” single-stranded DNA/RNA-binding proteins. However, the electro-negative surface of the SALPs suggests that neither SsgB nor any of the other SALPs are likely to interact with nucleotide substrates. Instead, we show that a conserved hydrophobic surface is likely to be important for SALP function and suggest that proteins are the likely binding partners. PMID:19567872

  8. Importancia de los aspectos psicosociales en la enfermedad celíaca

    Directory of Open Access Journals (Sweden)

    Trini Fragoso Arbelo

    2002-06-01

    Full Text Available Se revisan los conceptos clínicos actuales sobre la enfermedad celíaca. Se definen con precisión los términos enfermedad celíaca activa, silente, latente y potencial. Se enfatiza en los aspectos psicosociales que influyen en su tratamiento y se trazan pautas generales acerca del manejo integral de los pacientes afectados, dada la importancia de la dieta sin gluten de por vida como único tratamiento.The current clinical concepts of celiac disease are reviewed. The terms active, silent, latent and potential related to celiac disease are accurately defined. Emphasis is made on the psychosocial aspects influencing its treatment and general guidelines are drawn on the comprehensive management of the patients suffering from this disease, given the importance of diet without gluten as the only lifelong treatment.

  9. Improved Activity of a Thermophilic Cellulase, Cel5A, from Thermotoga maritima on Ionic Liquid Pretreated Switchgrass

    Science.gov (United States)

    Chen, Zhiwei; Pereira, Jose H.; Liu, Hanbin; Tran, Huu M.; Hsu, Nathan S. Y.; Dibble, Dean; Singh, Seema; Adams, Paul D.; Sapra, Rajat; Hadi, Masood Z.; Simmons, Blake A.; Sale, Kenneth L.

    2013-01-01

    Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification. However, even under these improved saccharification conditions the cost of enzymes still represents a significant proportion of the total cost of producing sugars and ultimately fuels from lignocellulosic biomass. Much of the high cost of enzymes is due to the low catalytic efficiency and stability of lignocellulolytic enzymes, especially cellulases, under conditions that include high temperatures and the presence of residual pretreatment chemicals, such as acids, organic solvents, bases, or ionic liquids. Improving the efficiency of the saccharification process on ionic liquid pretreated biomass will facilitate reduced enzyme loading and cost. Thermophilic cellulases have been shown to be stable and active in ionic liquids but their activity is typically at lower levels. Cel5A_Tma, a thermophilic endoglucanase from Thermotoga maritima, is highly active on cellulosic substrates and is stable in ionic liquid environments. Here, our motivation was to engineer mutants of Cel5A_Tma with higher activity on 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) pretreated biomass. We developed a robotic platform to screen a random mutagenesis library of Cel5A_Tma. Twelve mutants with 25–42% improvement in specific activity on carboxymethyl cellulose and up to 30% improvement on ionic-liquid pretreated switchgrass were successfully isolated and characterized from a library of twenty thousand variants. Interestingly, most of the mutations in the improved variants are located distally to the active site on the protein surface and are not directly involved with substrate binding. PMID:24244549

  10. Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass.

    Directory of Open Access Journals (Sweden)

    Zhiwei Chen

    Full Text Available Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification. However, even under these improved saccharification conditions the cost of enzymes still represents a significant proportion of the total cost of producing sugars and ultimately fuels from lignocellulosic biomass. Much of the high cost of enzymes is due to the low catalytic efficiency and stability of lignocellulolytic enzymes, especially cellulases, under conditions that include high temperatures and the presence of residual pretreatment chemicals, such as acids, organic solvents, bases, or ionic liquids. Improving the efficiency of the saccharification process on ionic liquid pretreated biomass will facilitate reduced enzyme loading and cost. Thermophilic cellulases have been shown to be stable and active in ionic liquids but their activity is typically at lower levels. Cel5A_Tma, a thermophilic endoglucanase from Thermotoga maritima, is highly active on cellulosic substrates and is stable in ionic liquid environments. Here, our motivation was to engineer mutants of Cel5A_Tma with higher activity on 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc] pretreated biomass. We developed a robotic platform to screen a random mutagenesis library of Cel5A_Tma. Twelve mutants with 25-42% improvement in specific activity on carboxymethyl cellulose and up to 30% improvement on ionic-liquid pretreated switchgrass were successfully isolated and characterized from a library of twenty thousand variants. Interestingly, most of the mutations in the improved variants are located distally to the active site on the protein surface and are not directly involved with substrate binding.

  11. Crystal structures of wild-type Trichoderma reesei Cel7A catalytic domain in open and closed states

    Energy Technology Data Exchange (ETDEWEB)

    Bodenheimer, Annette M. [Molecular and Structural Biochemistry Department, North Carolina State University, Raleigh NC USA; Neutron Sciences Directorate, Oak Ridge National Laboratory, TN USA; Meilleur, Flora [Molecular and Structural Biochemistry Department, North Carolina State University, Raleigh NC USA; Neutron Sciences Directorate, Oak Ridge National Laboratory, TN USA

    2016-11-07

    Trichoderma reesei Cel7A efficiently hydrolyses cellulose. We report here the crystallographic structures of the wild-type TrCel7A catalytic domain (CD) in an open state and, for the first time, in a closed state. Molecular dynamics (MD) simulations indicate that the loops along the CD tunnel move in concerted motions. Together, the crystallographic and MD data suggest that the CD cycles between the tense and relaxed forms that are characteristic of work producing enzymes. Analysis of the interactions formed by R251 provides a structural rationale for the concurrent decrease in product inhibition and catalytic efficiency measured for product-binding site mutants.

  12. AtGA3ox2, a key gene responsible for bioactive gibberellin biosynthesis, is regulated during embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in Arabidopsis

    NARCIS (Netherlands)

    Curaba, J.; Moritz, T.; Blervaque, R.; Parcy, F.; Raz, V.; Herzog, M.; Vachon, G.

    2004-01-01

    Embryonic regulators LEC2 (LEAFY COTYLEDON2) and FUS3 (FUSCA3) are involved in multiple aspects of Arabidopsis (Arabidopsis thaliana) seed development, including repression of leaf traits and premature germination and activation of seed storage protein genes. In this study, we show that gibberellin

  13. CEL-1 Lighting Computer Program - Programmer’s Guide.

    Science.gov (United States)

    1983-01-01

    COMPLETING FORM I REPORT NumeR .2 GOUT ACCESSION NO. 1. RECIPIENT’S CATALOG NUMBER CR 83.009� 4TITLE (Id Subt.II.) F HIf REOTAPIDCVRD CEL-1 Light...contribution due to the "bright spots" gene - rated in OVLY20 may be considered the "first bounce" effect. The ceiling contribution computed here in

  14. Descrição da artéria celíaca em pombos domésticos (Columba livia

    Directory of Open Access Journals (Sweden)

    Marcelo Ismar Santana

    2012-03-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n2p125 O presente estudo objetivou definir a origem e a distribuição da artéria celíaca e de seus ramos colaterais em 15 aves da espécie Columba livia, cedidas pelo Centro de Controle de Zoonoses de Brasília. Com a finalidade de marcar o sistema arterial dos espécimes, o tronco braquiocefálico esquerdo foi canulado e injetado com solução aquosa de látex corado. Posteriormente, procedeu-se a fixação das aves com solução aquosa de formol 10% e a dissecação com instrumentos adequados, obtendo-se os resultados a seguir. A artéria celíaca originou-se da face ventral da aorta descendente. O primeiro ramo colateral surgiu da própria artéria celíaca, constituindo a artéria esofágica. Posteriormente, artéria celíaca se bifurcou em dois ramos, denominados ramo esquerdo da artéria celíaca e ramo direito da artéria celíaca. O ramo esquerdo emitiu a artéria proventricular ventral, seguida das artérias esplênicas, da artéria proventricular dorsal e da artéria hepática esquerda. Por fim, o ramo esquerdo se bifurcou, originando as artérias gástricas ventral e esquerda. O ramo direito emitiu a artéria hepática direita, seguida da artéria ileal e da artéria gástrica direita. Por fim, o ramo direito prosseguiu como artéria pancreaticoduodenal. Os achados deste trabalho apresentaram grande similaridade com as linhagens de aves da espécie Gallus gallus, exceto pela ausência da artéria ileocecal, de ramos císticos e da artéria gástrica dorsal.

  15. Toxicity and antifeedant activity of essential oils from three aromatic plants grown in Colombia against Euprosterna elaeasa and Acharia fusca(Lepidoptera:Limacodidae)

    Institute of Scientific and Technical Information of China (English)

    Ricardo; Hernández-Lambrao; Karina; Caballero-Gallardo; Jesus; Olivero-Verbel

    2014-01-01

    Objective:To determine the biological effects of essential oils(EOs) isolated from Cymbopogon iiardus,Cymbopogon Jlexuosus and Cvrnbopogon marlinii grown in Colombia against two Lepidoptera larvae,common pests in the oil palm.Methods:Specimens were captured in the field and the antifeedant activity and dermal contact lethality of EOs were measured against Acharia fusca and Euprosterna elaeasa(Lepidoptera:I.imacodidae) at various concentrations 0.002-0.600 μL/cm~3 and 0.002-8 μL/g,respectively.Results:All EOs exhibited strong antifeedant and toxicity activity toward Acharia fusca and Euprosterna elaeasa larvae.Cymbopogon marlinii oil was llie most active againsl both pest insect species,although all tested EOs were better than the synthetic;repellent IR535 on both insects.Conclusions:Colombian EOs have potential for integrated pest management programs in the oil palm industry.

  16. Toxicity and antifeedant activity of essential oils from three aromatic plants grown in Colombia against Euprosterna elaeasa and Acharia fusca (Lepidoptera:Limacodidae)

    Institute of Scientific and Technical Information of China (English)

    Ricardo Hernández-Lambraño; Karina Caballero-Gallardo; Jesus Olivero-Verbel

    2014-01-01

    Objective:To determine the biological effects of essential oils (EOs) isolated from Cymbopogon nardus, Cymbopogon flexuosus and Cymbopogon martinii grown in Colombia against two Lepidoptera larvae, common pests in the oil palm. Methods:Specimens were captured in the field and the antifeedant activity and dermal contact lethality of EOs were measured against Acharia fusca and Euprosterna elaeasa (Lepidoptera:Limacodidae) at various concentrations 0.002-0.600 μL/cm2 and 0.002-8 μL/g, respectively. Results: All EOs exhibited strong antifeedant and toxicity activity toward Acharia fusca and Euprosterna elaeasa larvae. Cymbopogon martinii oil was the most active against both pest insect species, although all tested EOs were better than the synthetic repellent IR3535 on both insects. Conclusions:Colombian EOs have potential for integrated pest management programs in the oil palm industry.

  17. Cel vinyls: materials and methods.

    Science.gov (United States)

    Harrison, S J

    1983-12-01

    Many commercial uses of media are applicable to medical/scientific media production and illustration, not the least of which are techniques pioneered in the commercial cartooning field. Whether or not the illustrative effort culminates in a "cartoon," the production techniques of the cartooning industry cannot be overlooked by the illustrator faced with providing projection graphics, whether animated or still, for slides, motion pictures, television or even print media. When Walt Disney introduced Mickey Mouse as "Steamboat Willie" in 1928, his technology opened the door for exploration of cartooning media by all artists. Only in comparatively recent years have these tools been used by the scientific illustrator. In this article cel vinyl acrylics or cartoon colors will be discussed: the rationale for the use of this medium, materials and methods, and considerations related to the photography of this art form.

  18. Successful Treatment For Chronic Eosinophilic Leukemia (CEL With Imatinib Mesylate

    Directory of Open Access Journals (Sweden)

    Rayane da Silva Souza

    2017-12-01

    Full Text Available We report a case of a patient with Chronic Eosinophilic Leukemia (CEL with mutation in alfa PDGFR gene exhibiting a satisfactory response to treatment with imatinib mesylate. A 25-year-old man presented in a hematology service with a persistent cough and hemogram alterations. His blood count showed a hemoglobin level of 12.5 g/dL and a white blood cell count of 94,030/mm3, eosinophils were 68% of all cells. Bone marrow aspiration and biopsy showed hypercellularity with marked eosinophilia (77% and erythroid differentiation series was hypocellular with normoblast maturation. The immunohistochemically of the bone biopsy was positive for myeloperoxidase and negative for CD34/CD99, consistent with CEL. Fluorescence in situ hybridization (FISH for the beta-fraction of platelet-derived growth factor (PDGFRβ and Philadelphia chromosome (Ph 1 were negative and the alfa PDGFR (Platelet-Derived Growth Factor was positive and showed heterozygosis in c.2531T>C on 18 Exon and homozygous in C.2562+1G>A at the region of the splicing site at the 18 intron. Treatment was initiated and maintained by administering 400mg/day imatinib mesylate. Laboratory findings returned to normal ranges, with clinical improvement and a hematological response observed after the second month of therapy. Currently, the patient’s blood count shows the white blood cell count (5,400 total leukocytes, eosinophils (8.6/mm3, hemoglobin (15.5 g/dl, hematocrit (45.4% and platelets (298,000/mm3 within normal ranges. The mutation search was negative in in peripheral blood one year after the initial treatment. Our work corroborates other studies on the efficacy of imatinib mesylate in the treatment of patients with CSF PDGFR alpha positive. We emphasize the importance of molecular studies, considering its relevance for the correct staging of the disease. Since CEL is a rare disease, it is important to define its etiology and anticipate its treatment, thus minimizing the damage induced by

  19. Initial 2-year results of CardioCel® patch implantation in children.

    Science.gov (United States)

    Pavy, Carine; Michielon, Guido; Robertus, Jan Lukas; Lacour-Gayet, François; Ghez, Olivier

    2018-03-01

    We present the initial 2-year results of CardioCel® patch (Admedus Regen Pty Ltd, Perth, WA, Australia) implantation in paediatric patients with congenital heart diseases. This was a single-centre retrospective study with prospectively collected data of all patients aged 18 years and under operated for congenital heart disease. The patch was introduced in 2014, with clinical practice committee approval and a special consent in case of an Ozaki procedure. Standard follow-up was performed with systematic clinical exams and echocardiograms. In case of reoperation or graft failure, the patch was removed and sent for a histological examination. Between March 2014 and April 2016, 101 patients had surgical repair using a CardioCel patch. The mean age was 22 (±36.3) months, and the mean weight was 9.7 (±10.3) kg. No infections and no intraoperative implantation difficulties were associated with the patch. The median follow-up period was 212 (range 4-726) days. The overall 30-day postoperative mortality was 3.8% (n = 4), none of which were related to graft failure. Five children were reoperated because of graft failure, 4 of whom had the patch implanted for aortic and were aged less than 10 days. The indications for patch implantation in the aortic position were aortopulmonary window, truncus arteriosus, coarctation and aortic arch hypoplasia repair. The median time between the first and the second operation for graft failure was 245 (range 5-480) days. Our experience shows that the patch is well tolerated in the septal, valvar and pulmonary artery positions. However, we experienced graft failures in infants in the aortic position. © The Author 2017. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

  20. Age estimation and growth pattern of the island grouper, Mycteroperca fusca (Serranidae in an island population on the northwest coast of Africa

    Directory of Open Access Journals (Sweden)

    Rocío Bustos

    2009-06-01

    Full Text Available Mycteroperca fusca is one of the main predators of the Canarian archipelago coastal marine ecosystem. Nothing is known on the life history or population dynamics of this predatory serranid fish. In this study, age and growth of M. fusca were investigated by annual growth increment counts from 214 fish collected between January 2004 and December 2005. A year’s growth is represented by one opaque and one translucent ring. The island grouper is a slow-growing and long-lived species, with ages up to twenty years recorded. Length at age was described by the von Bertalanffy growth model (L∞=898 mm; k=0.062 year-1; and t0= −3.83 year, the Schnute growth model (y1=262 mm; y2=707 mm; a=0.008; and b=1.98, and the seasonalised von Bertalanffy growth model (L∞=908 mm; k=0.061 year-1; t0= −3.83 year; C=2.58; and ts=0.542. Males and females show differences in growth.

  1. Acute Toxicity and the Effects of Copper Sulphate (CuSO4.5H2O on the Behavior of the Black Fish (Capoeta Fusca

    Directory of Open Access Journals (Sweden)

    Iman Zarei

    2013-02-01

    Full Text Available Background: The development of toxicity tests regarding toxic responses of different fish species could be more effectively used in predictive toxicology and risk assessment. In this study lethal concentrations (LC50-96 h values of copper sulphate; an important toxic industrial pollutant, on Capoeta fusca were determined. Behavioral changes at different concentrations of CuSO4 were determined for the C.fusca. Methods: The sample fishes were collected from Qanat in Birjand and were transported to the laboratory in polythene bags. The exposure time of fish to CuSO4 was 96 hours. Mortalities were recorded at 24, 48, 72, and 96 hours of exposure, and the dead fish were removed regularly from the test aquariums. Physicochemical parameters, such as dissolved oxygen, pH and Total hardness of aquaria were monitored daily. Results: The LC50 values for CuSO4 at 24, 48, 72, and 96 h of exposure, were 43.62, 12.6, 7.66, and 6.85 mg/L, respectively. The median LC50 value of CuSO4 for C.fusca was found to be 6.928 mg/L by EPA method and estimated to be 6.787 mg/L with SPSS statistical software. Conclusion: The mortality decreased with time, and most of the deaths occurred during the first 24 h. In addition, behavioural changes increased with increased concentration. This metal is an important constituent in industrial effluents discharged into freshwaters. The results obtained in this study clearly revealed the fact that it is necessary to control the use of a heavy metal such as copper.

  2. Overlapping and distinct roles of AKIN10 and FUSCA3 in ABA and sugar signaling during seed germination

    OpenAIRE

    Tsai, Allen Yi-Lun; Gazzarrini, Sonia

    2012-01-01

    The Arabidopsis B3-domain transcription factor FUSCA3 (FUS3) is a master regulator of seed maturation and also a central modulator of hormonal responses during late embryogenesis and germination. Recently, we have identified AKIN10, the Arabidopsis ortholog of Snf1 (Sucrose Non-Fermenting-1)–Related Kinase1 (SnRK1), as a FUS3-interacting protein. We demonstrated that AKIN10 physically interacts with and phosphorylates FUS3 at its N-terminal region, and genetically interacts with FUS3 to regul...

  3. Temperature effects on kinetic parameters and substrate affinity of Cel7A cellobiohydrolases

    DEFF Research Database (Denmark)

    Sørensen, Trine Holst; Cruys-Bagger, Nicolaj; Windahl, Michael Skovbo

    2015-01-01

    Hypocrea jecorina and thermophilic Rasamsonia emersonii and two variants of these enzymes designed to elucidate the role of the carbohydrate binding module (CBM). We consistently found that the maximal rate increased strongly with temperature, whereas the affinity for the insoluble substrate decreased...... for affinity it slows down the catalytic process. Cel7A from the thermophilic organism was moderately more activated by temperature than the mesophilic analog. This is in accord with general theories on enzyme temperature adaptation and possibly relevant information for the selection of technical cellulases....

  4. Screening, cloning and expression analysis of a cellulase derived from the causative agent of hypertrophy sorosis scleroteniosis, Ciboria shiraiana.

    Science.gov (United States)

    Lü, Ruihua; Zhao, Aichun; Li, Jun; Liu, Changying; Wang, Chuanhong; Wang, Xiling; Wang, Xiaohong; Pei, Ruichao; Lu, Cheng; Yu, Maode

    2015-07-10

    A cellulase gene (KJ700939, CsCelA) from Ciboria shiraiana that is highly expressed during the infection of mulberry fruit was screened by quantitative real-time PCR (qRT-PCR). Using cDNA isolated from infected mulberry fruits as template, the full-length 1170-bp sequence of CsCelA was obtained, which encodes a 390-amino acid protein with a putative signal peptide of 24 amino acids. The 998-bp fragment encoding the mature peptide of CsCelA was cloned into the multiple cloning site of the pPIC9K vector and overexpressed as an active protein of 55.3kDa in the methylotrophic yeast Pichia pastoris. The specific activity of induced supernatants of the recombinant cellulase (CsCelA) was 17.44U/ml and 135U/g for freeze-dried powder. The Kmax and Vmax of CsCelA for sodium carboxymethylcellulose (CMC) were 4.6mg/ml and 107.2U/mg, respectively. The supernatant and freeze-dried powder of the recombinant cellulase exhibited stable activity from pH4.0 to 9.0, and at temperatures ranging from 30°C to 55°C. Finally, the activity of the recombinant cellulase was assessed by enzymatic hydrolysis of the cell walls of mulberry leaves. CsCelA showed an endo-cellulase mode of cleavage, as assessed by thin layer chromatography (TLC). Copyright © 2015 Elsevier B.V. All rights reserved.

  5. Mechanism of product inhibition for cellobiohydrolase Cel7A during hydrolysis of insoluble cellulose

    DEFF Research Database (Denmark)

    Olsen, Johan P.; Alasepp, Kadri; Kari, Jeppe

    2016-01-01

    The cellobiohydrolase cellulase Cel7A is extensively utilized in industrial treatment of lignocellulosic biomass under conditions of high product concentrations, and better understanding of inhibition mechanisms appears central in attempts to improve the efficiency of this process. We have...... the lines of conventional enzyme kinetic theory. We found that the product cellobiose lowered the maximal rate without affecting the Michaelis constant, and this kinetic pattern could be rationalized by two fundamentally distinct molecular mechanisms. One was simple reversibility, that is, an increasing...

  6. "9"0Sr in King Bolete Boletus edulis and certain other mushrooms consumed in Europe and China

    International Nuclear Information System (INIS)

    Saniewski, Michał; Zalewska, Tamara; Krasińska, Grażyna; Szylke, Natalia; Wang, Yuanzhong; Falandysz, Jerzy

    2016-01-01

    The "9"0Sr activity concentrations released from a radioactive fallout have been determined in a range of samples of mushrooms collected in Poland, Belarus, China, and Sweden in 1996–2013. Measurement of "9"0Sr in pooled samples of mushrooms was carried out with radiochemical procedure aimed to pre-isolate the analyte from the fungal materials before it was determined using the Low-Level Beta Counter. Interestingly, the Purple Bolete Imperator rhodopurpureus collected from Yunnan in south-western China in 2012 showed "9"0Sr activity concentration at around 10 Bq kg"−"1 dry biomass, which was greater when compared to other mushrooms in this study. The King Bolete Boletus edulis from China showed the "9"0Sr activity in caps at around 1.5 Bq kg"−"1 dry biomass (whole fruiting bodies) in 2012 and for specimens from Poland activity was well lower than 1.0 Bq kg"−"1 dry biomass in 1998–2010. A sample of Sarcodonimbricatus collected in 1998 from the north-eastern region of Poland impacted by Chernobyl fallout showed "9"0Sr in caps at around 5 Bq kg"−"1 dry biomass. Concentration of "9"0Sr in Bay Bolete Royoporus (Xerocomus or Boletus) badius from affected region of Gomel in Belarus was in 2010 at 2.1 Bq kg"−"1 dry biomass. In several other species from Poland "9"0Sr was at < 0.5 to around 1.0 Bq kg"−"1 dry biomass. Activity concentrations of "9"0Sr in popular B. edulis and some other mushrooms collected from wild in Poland were very low (< 1 Bq kg"−"1 dry biomass), and values noted showed on persistence of this type of radioactivity in mushrooms over time passing from nuclear weapons tests and the Chernobyl nuclear power plant catastrophe. - Highlights: • Mushrooms are an important food in some regions of the world. • Radioactive strontium ("9"0Sr) in mushrooms from Europe and China was measured. • Purple Bolete Imperator rhodopurpureus from Yunnan in China accumulates "9"0Sr. • "9"0Sr was at 11 ± 0 to 12 ± 0 Bq kg"−"1 dry biomass in caps of

  7. CelOWS: an ontology based framework for the provision of semantic web services related to biological models.

    Science.gov (United States)

    Matos, Ely Edison; Campos, Fernanda; Braga, Regina; Palazzi, Daniele

    2010-02-01

    The amount of information generated by biological research has lead to an intensive use of models. Mathematical and computational modeling needs accurate description to share, reuse and simulate models as formulated by original authors. In this paper, we introduce the Cell Component Ontology (CelO), expressed in OWL-DL. This ontology captures both the structure of a cell model and the properties of functional components. We use this ontology in a Web project (CelOWS) to describe, query and compose CellML models, using semantic web services. It aims to improve reuse and composition of existent components and allow semantic validation of new models.

  8. Prospektywny cel badania sprawozdania finansowego

    Directory of Open Access Journals (Sweden)

    Bronisław Micherda

    2010-05-01

    Full Text Available Problem wiarygodności sprawozdania finansowego oraz ocen i opinii z nim związa-nych odnosi się do trzech dziedzin: rachunkowości, analizy finansowej oraz badania sprawozdania finansowego. Szczególne znaczenie ma właściwe określenie celu ba-dania sprawozdania finansowego i jego uwarunkowania. Aktualnie biegły rewident powinien dokonać oceny nie tylko rozliczenia jednostki z otoczeniem, ale również po-dejmowanych przez nią decyzji, co przejawia się w możliwości kontynuacji działalności jednostki. Nakreślony cel badania sprawozdania finansowego stawia wyższe wymogi kom-petencyjne biegłym rewidentom. Szczególne znaczenie ma konieczność uświadomienia i szerszego wykorzystania analizy finansowej i planowania finansowego. Postulat modyfikacji regulacji prawnych celu badania sprawozdania finansowego zbiega się w czasie z ogłoszeniem Międzynarodowych Standardów Edukacyjnych IFAC, akcentu-jących kompetencje i etykę zawodowych księgowych.

  9. Immunization with Pre-Erythrocytic Antigen CelTOS from Plasmodium falciparum Elicits Cross-Species Protection against Heterologous Challenge with Plasmodium berghei

    Science.gov (United States)

    2010-08-01

    or the early liver-stages of the mammalian life cycle . One of these antigens is the cell-traversal protein for ookinetes and sporozoites (CelTOS...Immunization with Pre-Erythrocytic Antigen CelTOS from Plasmodium falciparum Elicits Cross-Species Protection against Heterologous Challenge with... Plasmodium berghei Elke S. Bergmann-Leitner1*, Ryan M. Mease1, Patricia De La Vega1, Tatyana Savranskaya2, Mark Polhemus1, Christian Ockenhouse1, Evelina

  10. La enfermedad celíaca se podría detectar con una determinación de anticuerpos antitransglutaminasa en la saliva

    OpenAIRE

    Cuestas Montañés, Eduardo José; Ortega Páez, Eduardo

    2011-01-01

    Este estudio avanza en la posibilidad de realizar una prueba de cribado simple e inocua para detectar la enfermedad celíaca a edades tempranas, lo que puede contribuir a disminuir la morbimortalidad asociada a la enfermedad.

  11. Identification of JAK2 as a mediator of FIP1L1-PDGFRA-induced eosinophil growth and function in CEL.

    Directory of Open Access Journals (Sweden)

    Bin Li

    Full Text Available The Fip1-like1 (FIP1L1-platelet-derived growth factor receptor alpha fusion gene (F/P arising in the pluripotent hematopoietic stem cell (HSC,causes 14% to 60% of patients with hypereosinophilia syndrome (HES. These patients, classified as having F/P (+ chronic eosinophilic leukemia (CEL, present with clonal eosinophilia and display a more aggressive disease phenotype than patients with F/P (- HES patients. The mechanisms underlying predominant eosinophil lineage targeting and the cytotoxicity of eosinophils in this leukemia remain unclear. Given that the Janus tyrosine kinase (JAK/signal transducers and activators of transcription (Stat signaling pathway is key to cytokine receptor-mediated eosinophil development and activated Stat3 and Stat5 regulate the expression of genes involved in F/P malignant transformation, we investigated whether and how JAK proteins were involved in the pathogenesis of F/P-induced CEL. F/P activation of JAK2, Stat3 and Stat5, were confirmed in all the 11 F/P (+ CEL patients examined. In vitro inhibition of JAK2 in EOL-1, primary F/P(+ CEL cells (PC and T674I F/P Imatinib resistant cells(IR by either JAK2-specific short interfering RNA (siRNA or the tryphostin derivative AG490(AG490, significantly reduced cellular proliferation and induced cellular apoptosis. The F/P can enhance the IL-5-induced JAK2 activation, and further results indicated that JAK2 inhibition blocked IL-5-induced cellular migration and activation of the EOL-1 and PC cells in vitro. F/P-stimulation of the JAK2 suppressed cells led to a significantly reduction in Stat3 activation, but relatively normal induction of Stat5 activation. Interestingly, JAK2 inhibition also reduced PI3K, Akt and NF-κB activity in a dose-dependent manner, and suppressed expression levels of c-Myc and Survivin. These results strongly suggest that JAK2 is activated by F/P and is required for F/P stimulation of cellular proliferation and infiltration, possibly through

  12. Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution.

    Science.gov (United States)

    Varrot, A; Hastrup, S; Schülein, M; Davies, G J

    1999-01-15

    The three-dimensional structure of the catalytic core of the family 6 cellobiohydrolase II, Cel6A (CBH II), from Humicola insolens has been determined by X-ray crystallography at a resolution of 1.92 A. The structure was solved by molecular replacement using the homologous Trichoderma reesei CBH II as a search model. The H. insolens enzyme displays a high degree of structural similarity with its T. reesei equivalent. The structure features both O- (alpha-linked mannose) and N-linked glycosylation and a hexa-co-ordinate Mg2+ ion. The active-site residues are located within the enclosed tunnel that is typical for cellobiohydrolase enzymes and which may permit a processive hydrolysis of the cellulose substrate. The close structural similarity between the two enzymes implies that kinetics and chain-end specificity experiments performed on the H. insolens enzyme are likely to be applicable to the homologous T. reesei enzyme. These cast doubt on the description of cellobiohydrolases as exo-enzymes since they demonstrated that Cel6A (CBH II) shows no requirement for non-reducing chain-ends, as had been presumed. There is no crystallographic evidence in the present structure to support a mechanism involving loop opening, yet preliminary modelling experiments suggest that the active-site tunnel of Cel6A (CBH II) is too narrow to permit entry of a fluorescenyl-derivatized substrate, known to be a viable substrate for this enzyme.

  13. Screening for celiac disease among patients with Turner syndrome in Brasília, DF, midwest region of Brazil Triagem para doença celíaca em pacientes com síndrome de Turner em Brasília, DF, região centro-oeste do Brasil

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Sorci Dias

    2010-09-01

    Full Text Available CONTEXT: Several studies have demonstrated a higher prevalence of celiac disease (CD among females with Turner syndrome when compared to the general population. Nevertheless, there is no record in literature concerning this investigation among Brazilian patients. OBJECTIVE: To assess the prevalence of CD among a group of Brazilian patients with Turner syndrome. METHODS: Fifty-six females with Turner syndrome and on gluten-containing diet were screened for CD utilizing immunoglobulin A antiendomysium (IgA-EMA and immunoglobulin A anti-tissue transglutaminase (IgA-tTG antibody assays. Additionally, they were genotyped for CD human leukocyte antigen (CD-HLA predisposing alleles. Patients showing positivity in serological testing were offered to perform small intestine biopsy for histological confirmation. RESULTS: Mean age at diagnosis of Turner syndrome was 5.5 ± 4.4 years; mean age at screening for CD was 17.0 ± 9.3 years (from 10 months of age to 52 years. Two girls were positive for IgA-EMA and IgA-tTG, presented predisposing HLA-DQ2 alleles and both had the diagnosis of CD confirmed by jejunal biopsy. CONCLUSION: The 3.6% prevalence of biopsy-proven CD among this group of females with Turner syndrome is 10 times higher than the one among females from the general population of the same geographical area. This result provides additional support to an association between these two disorders and restates that girls and women with Turner syndrome represent a high risk population for developing CD.CONTEXTO: Alguns estudos têm demonstrado maior prevalência de doença celíaca entre mulheres com síndrome de Turner, quando comparadas com a população geral. Entretanto, não há registro na literatura desta investigação em pacientes brasileiras. OBJETIVO: Avaliar a prevalência de doença celíaca entre um grupo de pacientes brasileiras com síndrome de Turner. MÉTODOS: Cinquenta e seis pacientes com síndrome de Turner recebendo dieta contendo

  14. Doença celíaca associada à tireoidite de Hashimoto e síndrome de Noonan

    OpenAIRE

    Perez,Mariana Ortega; Ciambelli,Giuliano Serafino; Nigri,Alcinda Aranha; Vieira,Marta Wey; Costa,Clóvis Duarte

    2010-01-01

    OBJETIVO: Relatar o caso clínico de uma criança portadora de doença celíaca, tireoidite de Hashimoto e síndrome de Noonan. DESCRIÇÃO DE CASO: Menina de dez anos e seis meses, branca, apresentando história de diarreia líquida há cinco meses e "aumento da barriga". Ao exame, mostrava peso de 20.580g (p

  15. Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A

    DEFF Research Database (Denmark)

    Cruys-Bagger, Nicolaj; Olsen, Jens Elmerdahl; Præstgaard, Eigil

    2012-01-01

    The transient kinetic behavior of enzyme reactions prior to the establishment of steady state is a major source of mechanistic information, yet this approach has not been utilized for cellulases acting on their natural substrate, insoluble cellulose. Here, we elucidate the pre-steady-state regime...... for the exo-acting cellulase Cel7A using amperometric biosensors and an explicit model for processive hydrolysis of cellulose. This analysis allows the identification of a pseudo-steady-state period and quantification of a processivity number as well as rate constants for the formation of a threaded enzyme...... to unveil fundamental reasons for the distinctive variability in hydrolytic activity found in different cellulase-substrate systems....

  16. Length of Variable Numbers of Tandem Repeats in the Carboxyl Ester Lipase (CEL) Gene May Confer Susceptibility to Alcoholic Liver Cirrhosis but Not Alcoholic Chronic Pancreatitis.

    Science.gov (United States)

    Fjeld, Karianne; Beer, Sebastian; Johnstone, Marianne; Zimmer, Constantin; Mössner, Joachim; Ruffert, Claudia; Krehan, Mario; Zapf, Christian; Njølstad, Pål Rasmus; Johansson, Stefan; Bugert, Peter; Miyajima, Fabio; Liloglou, Triantafillos; Brown, Laura J; Winn, Simon A; Davies, Kelly; Latawiec, Diane; Gunson, Bridget K; Criddle, David N; Pirmohamed, Munir; Grützmann, Robert; Michl, Patrick; Greenhalf, William; Molven, Anders; Sutton, Robert; Rosendahl, Jonas

    2016-01-01

    Carboxyl-ester lipase (CEL) contributes to fatty acid ethyl ester metabolism, which is implicated in alcoholic pancreatitis. The CEL gene harbours a variable number of tandem repeats (VNTR) region in exon 11. Variation in this VNTR has been linked to monogenic pancreatic disease, while conflicting results were reported for chronic pancreatitis (CP). Here, we aimed to investigate a potential association of CEL VNTR lengths with alcoholic CP. Overall, 395 alcoholic CP patients, 218 patients with alcoholic liver cirrhosis (ALC) serving as controls with a comparable amount of alcohol consumed, and 327 healthy controls from Germany and the United Kingdom (UK) were analysed by determination of fragment lengths by capillary electrophoresis. Allele frequencies and genotypes of different VNTR categories were compared between the groups. Twelve repeats were overrepresented in UK ACP patients (P = 0.04) compared to controls, whereas twelve repeats were enriched in German ALC compared to alcoholic CP patients (P = 0.03). Frequencies of CEL VNTR lengths of 14 and 15 repeats differed between German ALC patients and healthy controls (P = 0.03 and 0.008, respectively). However, in the genotype and pooled analysis of VNTR lengths no statistical significant association was depicted. Additionally, the 16-16 genotype as well as 16 repeats were more frequent in UK ALC than in alcoholic CP patients (P = 0.034 and 0.02, respectively). In all other calculations, including pooled German and UK data, allele frequencies and genotype distributions did not differ significantly between patients and controls or between alcoholic CP and ALC. We did not obtain evidence that CEL VNTR lengths are associated with alcoholic CP. However, our results suggest that CEL VNTR lengths might associate with ALC, a finding that needs to be clarified in larger cohorts.

  17. MinION Analysis and Reference Consortium: Phase 2 data release and analysis of R9.0 chemistry.

    Science.gov (United States)

    Jain, Miten; Tyson, John R; Loose, Matthew; Ip, Camilla L C; Eccles, David A; O'Grady, Justin; Malla, Sunir; Leggett, Richard M; Wallerman, Ola; Jansen, Hans J; Zalunin, Vadim; Birney, Ewan; Brown, Bonnie L; Snutch, Terrance P; Olsen, Hugh E

    2017-01-01

    Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Escherichia coli K-12 using the R9.0 chemistry, comparing the results with the older R7.3 chemistry. We computed the error-rate estimates for insertions, deletions, and mismatches in MinION reads. Run-time characteristics of the flow cell and run scripts for R9.0 were similar to those observed for R7.3 chemistry, but with an 8-fold increase in bases per second (from 30 bps in R7.3 and SQK-MAP005 library preparation, to 250 bps in R9.0) processed by individual nanopores, and less drop-off in yield over time. The 2-dimensional ("2D") N50 read length was unchanged from the prior chemistry. Using the proportion of alignable reads as a measure of base-call accuracy, 99.9% of "pass" template reads from 1-dimensional ("1D")  experiments were mappable and ~97% from 2D experiments. The median identity of reads was ~89% for 1D and ~94% for 2D experiments. The total error rate (miscall + insertion + deletion ) decreased for 2D "pass" reads from 9.1% in R7.3 to 7.5% in R9.0 and for template "pass" reads from 26.7% in R7.3 to 14.5% in R9.0. These Phase 2 MinION experiments serve as a baseline by providing estimates for read quality, throughput, and mappability. The datasets further enable the development of bioinformatic tools tailored to the new R9.0 chemistry and the design of novel biological applications for this technology. K: thousand, Kb: kilobase (one thousand base pairs), M: million, Mb: megabase (one million base pairs), Gb: gigabase (one billion base pairs).

  18. SPSS 9.0 for Windows 98/NT统计软件包简介

    Institute of Scientific and Technical Information of China (English)

    李志辉; 洪楠

    2000-01-01

    @@ SPSS(Statistical Package for the Social Science)是由美国SPSS公司自80年代初开发的大型统计学软件包.1998年推出的SPSS 9.0版本,除保持了以往SPSS的诸多优点以外,还汇集了SAS for Windows[1,2]与STATISTICA for Windows95/NT[3,4]的长处,是当今世界上最新、最流行、最受欢迎的统计软件包之一.与SPSS 7.5比较,SPSS 9.0具有以下新的特点[5].

  19. La anemia ferropénica como presentación de enfermedad celíaca subclínica en una población argentina

    OpenAIRE

    J.S. Lasa; P. Olivera; L. Soifer; R. Moore

    2017-01-01

    Introducción: Existe una extensa heterogeneidad en los reportes de la prevalencia de enfermedad celíaca en el contexto de la anemia ferropénica. Objetivo: Determinar la prevalencia de enfermedad celíaca en pacientes con anemia ferropénica. Materiales y métodos: Pacientes adultos con anemia ferropénica fueron reclutados para realizarse una endoscopia digestiva alta con biopsia duodenal. Se reclutaron asimismo voluntarios sanos de la comunidad como controles. Resultados: Se reclutó a 1...

  20. Aspectos genéticos e imunopatogênicos da doença celíaca: visão atual Genetics and immunopathogenics aspects of the celiac disease: a recent vision

    Directory of Open Access Journals (Sweden)

    Shirley Ramos da Rosa Utiyama

    2004-06-01

    Full Text Available RACIONAL: A doença celíaca ou enteropatia por sensibilidade ao glúten, é uma forte condição hereditária. Embora a associação genética da doença celíaca com os haplótipos HLA-DQ2 e DQ8 seja conhecida há muito tempo, outros genes HLA e não-HLA também são importantes no desenvolvimento da afecção. A doença celíaca resulta de um efeito combinado de produtos de diferentes genes funcionantes normalmente. A lesão intestinal é imunologicamente mediada e múltiplos mecanismos efetores são responsáveis pela sua expressão. A interação entre fatores genéticos, imunológicos e ambientais explicam o amplo espectro de alterações clínicas, histológicas e sorológicas observadas nos diferentes estágios de desenvolvimento da doença, ressaltando a natureza poligênica da mesma. CONCLUSÃO: Os avanços recentes na compreensão da imunopatogenia, genética e diagnóstico da doença celíaca têm permitido que rígidos conceitos e critérios pré-estabelecidos sejam revistos e adequados às novas evidências, visando melhor diagnóstico e orientação para pacientes celíacos e familiares.BACKGROUND: Celiac disease, or gluten-sensitive enteropathy, is a strongly inherited condition. Although the genetic association of CD with the DQ2 and DQ8 HLA haplotypes has been known for long, others HLA and non-HLA genes are also important in the development of the disease. Celiac disease results of the combined effect of different normally functioning genes' products. The tissue damage in celiac disease is immunologically mediated and several effector mechanisms are responsible for the disease expression. The interplay between genetic, immunological and environmental factors explains the large spectrum of clinical, histological and serological alterations observed in the different stages of the disease development, pointing out to the polygenic nature of celiac disease. CONCLUSION: The recent advances in the understanding of the

  1. Isolation and characterization of two different flavodoxins from the eukaryote Chlorella fusca.

    Science.gov (United States)

    Peleato, M L; Ayora, S; Inda, L A; Gómez-Moreno, C

    1994-01-01

    Two different molecular forms of flavodoxin from the green alga Chlorella fusca have been purified to homogeneity and their properties compared. The molecular masses are 22 kDa (flavodoxin I) and 20 kDa (flavodoxin II). Western blots of axenic crude extract show the two bands. Both are single polypeptide chains and their N-terminal sequences differ but are very similar. Each form contains 1 mol of FMN/mol of apoprotein, exhibits a typical flavodoxin u.v.-visible absorption spectrum and does not contain covalently bound phosphate. The oxidation-reduction properties of the FMN in the flavodoxins differ considerably. Redox potentials of flavodoxin I at pH8 are -240 mV for the oxidized/semiquinone couple and -350 mV for the semiquinone/hydroquinone couple. Flavodoxin II gives more electronegative values: -278 mV and -458 mV respectively. Flavodoxin II fulfils better the redox requirements for photosynthetic electron transport and, as expected, it is more efficient at mediating NADP+ photoreduction in the photosynthetic electron flow. A new h.p.l.c. method for flavodoxin purification is described, which is useful for the isolation of very similar anionic proteins. Images Figure 2 Figure 3 PMID:7945206

  2. Crianças e adolescentes que convivem com diabetes e doença celíaca

    OpenAIRE

    Brancaglioni, Bianca de Cássia Alvarez; Rodrigues, Grasiele Caroline; Damião, Elaine Buchhorn Cintra; Queiroz, Márcia Silva; Nery, Márcia

    2016-01-01

    RESUMO Objetivo Compreender a experiência de crianças e adolescentes que convivem com diabetes mellitus tipo 1 e doença celíaca. Método Estudo qualitativo, exploratório e descritivo. A coleta de dados ocorreu entre janeiro e setembro de 2012, com 3 crianças e 2 adolescentes, em um ambulatório de diabetes do Hospital das Clinicas da FMUSP ou na residência dos participantes na cidade de São Paulo, por meio de entrevistas semiestruturadas. Utilizou-se a Análise de Conteúdo como método de tra...

  3. Simulation analysis of the cellulase Cel7A carbohydrate binding module on the surface of the cellulose Iβ

    Energy Technology Data Exchange (ETDEWEB)

    Alekozai, Emal M. [Univ. of Heidelberg (Germany); Univ. of Tennessee, Knoxville, TN (United States); GhattyVenkataKrishna, Pavan K. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Uberbacher, Edward C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Crowley, Michael F. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States); Smith, Jeremy C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Cheng, Xiaolin [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

    2013-08-22

    The Family 7 cellobiohydrolase (Cel7A) from Trichoderma reesei consists of a carbohydrate-binding module (CBM) joined by a linker to a catalytic domain. Cellulose hydrolysis is limited by the accessibility of Cel7A to crystalline substrates, which is perceived to be primarily mediated by the CBM. The binding of CBM to the cellulose I fiber is characterized by combined Brownian dynamics (BD) and molecular dynamics (MD) simulations. Our results confirm that CBM prefers to dock to the hydrophobic than to the hydrophilic fiber faces. Both electrostatic (ES) and van der Waals (VDW) interactions are required for achieving the observed binding preference. The VDW interactions play a more important role in stabilizing the CBM-fiber binding, whereas the ES interactions contribute through the formation of a number of hydrogen bonds between the CBM and the fiber. At long distances, an ES steering effect is also observed that tends to align the CBM in an antiparallel manner relative to the fiber axis. Moreover, the MD results reveal hindered diffusion of the CBM on all fiber surfaces. The binding of the CBM to the hydrophobic surfaces is found to involve partial dewetting at the CBM-fiber interface coupled with local structural arrangements of the protein. The present simulation results complement and rationalize a large body of previous work and provide detailed insights into the mechanism of the CBM-cellulose fiber interactions.

  4. Comparison of traditional field retting and Phlebia radiata Cel 26 retting of hemp fibres for fibre-reinforced composites

    DEFF Research Database (Denmark)

    Liu, Ming; Ale, Marcel Tutor; Kołaczkowski, Bartłomiej

    2017-01-01

    Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical properties of the produced fibre/epoxy composites. For field retting a classification...

  5. Adaptação nutricional diante da doença celíaca desencadeada pela intolerância ao glúten

    Directory of Open Access Journals (Sweden)

    Francileuda Batista de Almeida

    2016-01-01

    Full Text Available A intolerância ao glúten que desencadeia a doença celíaca DC tem analises e estudo a mais de dois séculos, glúten proteína presente nas farinhas, o doente celíaco tem como sintomas distúrbios gastrointestinais ente outros problemas de saúde decorrente de dietas a parti de alimentos que contem glúten. A partir de tais pressupostos, pretendeu-se com o trabalho de revisão, analisar a necessidade de adaptações nutricionais mediante a doença celíaca desencadeada pela intolerância ao glúten. Neste sentido, tem-se que os sintomas apesentam de formas típicas e atípicas. Fatores intrínsecos também contribuem para o problema tais com os imunológicos, genéticos e ambientais. Estudos comprovam que para melhor convivência com o problema o paciente celíaco deve obter todas as informações tanto do corpo clínico que lhe assiste quanto dos exames realizados como e o caso da biopsia do intestino. O tratamento se baseia em ingestão de dieta sem glúten, antes era difícil encontrar no mercado mais hoje já existem formulações sem glúten bolos goma xantana e biscoito de farrinha de palmeia real. Este trabalho descreve em formato de revisão bibliográfica este assunto.Nutritional adaptation faced with celiac disease triggered by intolerance to glutenAbstract: The intolerance to gluten that triggers the DC celiac disease has analysis and study more than two centuries, gluten protein found in flour, the patient celiac has the symptoms gastrointestinal being disorders other health problems due to diet the left of foods containing gluten. Based on these assumptions, it was intended with the revision work, examine the need for nutritional adaptations by celiac disease triggered by intolerance to gluten-. In this sense, it has to be apesentam symptoms of typical and atypical forms. Intrinsic factors also contribute to the problem with such immunological, genetic and environmental. Studies show that for better interaction with the patient

  6. Inter-laboratory exercise with an aim to compare methods for "9"0Sr and "2"3"9","2"4"0Pu determination in environmental soil samples

    International Nuclear Information System (INIS)

    Jixin Qiao; Per Roos; Salminen-Paatero, Susanna; Stina Holmgren Rondahl; Lagerkvist, Petra; Bourgeaux-Goget, Marie; Stralberg, Elisabeth; Rameback, Henrik; Chalmers University of Technology, Gothenburg

    2017-01-01

    In order to deliver reliable results for a multitude of different scenarios, e.g. emergency preparedness, environmental monitoring, nuclear decommissioning and waste management, there is a constant process of method development in the field of radioanalytical chemistry. This work presents the results of a method comparison exercise aimed at quantifying "9"0Sr and "2"3"9","2"4"0Pu in environmental soil samples, with the intention of evaluating the performance and applicability of different methods. From the methods examined in this work, recommendations are given in order to find a radioanalytical measurement procedure, for "9"0Sr and "2"3"9","2"4"0Pu analysis, which is fit-for-purpose for a particular scenario. (author)

  7. Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation

    Directory of Open Access Journals (Sweden)

    Moraïs Sarah

    2012-10-01

    Full Text Available Abstract Background Microorganisms employ a multiplicity of enzymes to efficiently degrade the composite structure of plant cell wall cellulosic polysaccharides. These remarkable enzyme systems include glycoside hydrolases (cellulases, hemicellulases, polysaccharide lyases, and the carbohydrate esterases. To accomplish this challenging task, several strategies are commonly observed either separately or in combination. These include free enzyme systems, multifunctional enzymes, and multi-enzyme self-assembled designer cellulosome complexes. Results In order to compare these different paradigms, we employed a synthetic biology approach to convert two different cellulases from the free enzymatic system of the well-studied bacterium, Thermobifida fusca, into bifunctional enzymes with different modular architectures. We then examined their performance compared to those of the combined parental free-enzyme and equivalent designer-cellulosome systems. The results showed that the cellulolytic activity displayed by the different architectures of the bifunctional enzymes was somewhat inferior to that of the wild-type free enzyme system. Conclusions The activity exhibited by the designer cellulosome system was equal or superior to that of the free system, presumably reflecting the combined proximity of the enzymes and high flexibility of the designer cellulosome components, thus enabling efficient enzymatic activity of the catalytic modules.

  8. Virus-like particle nanoreactors: programmed en capsulation of the thermostable CelB glycosidase inside the P22 capsid

    NARCIS (Netherlands)

    Patterson, D.P.; Schwarz, B.; El-Boubbou, K.; Oost, van der J.; Prevelige, P.E.; Douglas, T.

    2012-01-01

    Self-assembling biological systems hold great potential for the synthetic construction of new active soft nanomaterials. Here we demonstrate the hierarchical bottom-up assembly of bacteriophage P22 virus-like particles (VLPs) that encapsulate the thermostable CelB glycosidase creating catalytically

  9. A Specific Mutation in the Promoter Region of the Silent cel Cluster Accounts for the Appearance of Lactose-Utilizing Lactococcus lactis MG1363

    Science.gov (United States)

    Solopova, Ana; Bachmann, Herwig; Teusink, Bas; Kok, Jan; Neves, Ana Rute

    2012-01-01

    The Lactococcus lactis laboratory strain MG1363 has been described to be unable to utilize lactose. However, in a rich medium supplemented with lactose as the sole carbon source, it starts to grow after prolonged incubation periods. Transcriptome analyses showed that L. lactis MG1363 Lac+ cells expressed celB, encoding a putative cellobiose-specific phosphotransferase system (PTS) IIC component, which is normally silent in MG1363 Lac− cells. Nucleotide sequence analysis of the cel cluster of a Lac+ isolate revealed a change from one of the guanines to adenine in the promoter region. We showed here that one particular mutation, taking place at increased frequency, accounts for the lactose-utilizing phenotype occurring in MG1363 cultures. The G-to-A transition creates a −10 element at an optimal distance from the −35 element. Thus, a fully active promoter is created, allowing transcription of the otherwise cryptic cluster. Nuclear magnetic resonance (NMR) spectroscopy results show that MG1363 Lac+ uses a novel pathway of lactose utilization. PMID:22660716

  10. Use of xylazine hydrochloride-ketamine hydrochloride for immobilization of wild leopards (Panthera pardus fusca) in emergency situations.

    Science.gov (United States)

    Belsare, Aniruddha V; Athreya, Vidya R

    2010-06-01

    In India, leopards (Panthera pardus fusca) inhabit human-dominated landscapes, resulting in encounters that require interventions to prevent harm to people, as well as the leopards. Immobilization is a prerequisite for any such intervention. Such emergency field immobilizations have to be carried out with limited tools, often amidst large uncontrollable crowds. An effective and practicable approach is discussed, based on 55 wild leopard immobilizations undertaken between January 2003 and April 2008. A xylazine hydrochloride (1.4 +/- 0.3 mg/kg)--ketamine hydrochloride (5 +/- 2 mg/kg) mixture was used for immobilization of leopards, based on estimated body weight. When weight could not be estimated, a standard initial dose of 50 mg of xylazine--150 mg of ketamine was used. Supplemental doses (50-75 mg) of only ketamine were used as required. No life-threatening adverse effects of immobilization were documented for at least 1 mo postimmobilization.

  11. Optimization of ruminococcus albus endoglucanase cel5-cbm6 production in plants by incorporating an elp tag and targeting to different subcellular compartments

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, E.O.; Menassa, R. [Western Ontario Univ., London, ON (Canada). Dept. of Biology; Agriculture and Agri-Food Canada, London, ON (Canada); Kolotilin, I. [Agriculture and Agri-Food Canada, London, ON (Canada)

    2009-07-01

    The production of biomass-based biofuel such as ethanol depends on the deconstruction of a cellulosic matrix and requires a variety of enzymes that hydrolyze glycosidic bonds to release fermentable sugars. Endoglucanases are one of most important groups of natural cellulosic hydrolytic enzymes that act on cellulose. In order to decrease ethanol production costs, the cost of producing cellulases must also be reduced. Genetically engineered transgenic plants are among the most economical systems for large scale production of recombinant proteins because of the large amount of enzymes that can be produced with minimal input. Cellulases present different levels of expression in different subcellular compartments. Cel5-CBM6 is a fused protein containing an endocellulase from Ruminococus albus (Cel5) and a cellulose binding domain (CBD) of Clostridium stercorarium. It accumulates in both the chloroplast and cytoplasm, but severe growth defects occur when expressed in the cytoplasm. Therefore, other subcellular compartments such as endoplasmic reticulum (ER) and vacuole must be evaluated and compared to determine the best co partment for production and activity of cellulases. Since elastin-like polypeptide (ELP) has also been shown to increase recombinant protein accumulation in plants, this study evaluated the effects of incorporating an ELP tag and a retrieval signal peptide on the expression levels of Cel5-CBM6.

  12. Estudi de l'efecte de diferents pre-tractaments químics, mecànics i biotecnològics en l'oxidació de fibres cel·lulòsiques mitjançant ozó

    OpenAIRE

    Junyent Blanco, Ana

    2017-01-01

    El següent treball té com a principal objectiu oxidar les fibres cel·lulòsiques blanquejades d’eucaliptus mitjançant ozó per tal d'obtenir una cel·lulosa modificada. Primerament s’avaluen diferents dosis d’ozó així com l’efecte del rentat entre dosis per tal d’obtenir la màxima oxidació possible. Aquesta s’avalua tant a partir de la viscositat de la pasta i els talls que es produeixen a la cel·lulosa, l’envelliment, així com a partir de la mesura de grups aldehid i carboxil. Seguidament, s’es...

  13. Five Year Results of US Intergroup/RTOG 9704 With Postoperative CA 19-9 {<=}90 U/mL and Comparison to the CONKO-001 Trial

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Adam C., E-mail: adam.berger@jefferson.edu [Thomas Jefferson University Hospital, Philadelphia, Pennsylvania (United States); Winter, Kathryn [RTOG Statistical Center, Philadelphia, Pennsylvania (United States); Hoffman, John P. [Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Regine, William F. [University of Maryland Medical Systems, Baltimore, Maryland (United States); Abrams, Ross A. [Rush University Medical Center, Chicago, Illinois (United States); Safran, Howard [Division of Hematology/Oncology, The Miriam Hospital, Providence, Rhode Island (United States); Freedman, Gary M. [Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Benson, Alan B. [Northwestern Memorial Hospital, Chicago, Illinois (United States); MacDonald, John [St. Vincent' s Comprehensive Cancer Center, New York, New York (United States); Willett, Christopher G. [Duke University Medical Center, Durham, North Carolina (United States)

    2012-11-01

    Purpose: Radiation Therapy Oncology Group (RTOG) trial 9704 was the largest randomized trial to use adjuvant chemoradiation therapy for patients with pancreatic cancer. This report analyzes 5-year survival by serum level of tumor marker CA 19-9 of {<=}90 vs >90 U/mL and compares results to the those of the CONKO-001 trial. Methods and Materials: CA 19-9 expression was analyzed as a dichotomized variable ({<=}90 vs >90 U/mL). Cox proportional hazard models were used to identify the impact of the CA 19-9 value on overall survival (OS). Actuarial estimates of OS were calculated using the Kaplan-Meier method. Results: Both univariate (hazard ratio [HR] = 3.2; 95% confidence interval [CI], 2.3-4.3, P<.0001) and multivariate (HR = 3.1; 95% CI, 2.2-4.2, P<.0001) analyses demonstrated a statistically significant decrease in OS for CA 19-9 serum level of {>=}90 U/mL. For patients in the gemcitabine (Gem) treatment arm with CA 19-9 <90 U/mL, median survival was 21 months. For patients with CA 19-9 {>=}90 U/mL, this number dropped to 10 months. In patients with pancreatic head tumors in the Gem treatment arm with RT quality assurance per protocol and CA 19-9 of <90 U/mL, median survival and 5-year rate were 24 months and 34%. In comparison, the median survival and 5-year OS rate for patients in the Gem arm of the CONKO trial were 22 months and 21%. Conclusions: This analysis demonstrates that patients with postresection CA 19-9 values {>=}90 U/mL had a significantly worse survival. Patients with pancreatic head tumors treated with Gem with CA 19-9 serum level of <90 U/mL and per protocol RT had favorable survival compared to that seen in the CONKO trial. CA 19-9 is a stratification factor for the current RTOG adjuvant pancreas trial (0848).

  14. Laser program development at CEL-V: overview of recent experimental results

    International Nuclear Information System (INIS)

    Buresi, E.; Coutant, J.; Dautray, R.

    1985-11-01

    A significant effort has been made recently at CEL-V to improve laser facilities. OCTAL, the eight beam, 2 kJ laser, has been equipped with phosphate glass and KDP frequency tripling systems. PHEBUS, a two beam 20 kJ neodymium glass laser based on NOVA technology was defined, built and will be tested in early 1986 in close collaboration with Lawrence Livermore National laboratory. In the field of diagnostics, the development of soft X-ray emission analysis has been emphasized. Most of recent experimental results have been obtained at short wave-length (0.35 μm). They deal with: effect of non-uniform illumination, 2D hydrodynamics with either plane or spherical targets, and study of thermal transport inhibition

  15. Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense

    NARCIS (Netherlands)

    Gusakov, A.V.; Sinitsyn, A.P.; Salanovich, T.N.; Bukhtojarov, F.E.; Markov, A.V.; Ustinov, B.B.; Zeijl, C.V.; Punt, P.; Burlingame, R.

    2005-01-01

    Two forms of cellobiohydrolase I (CBH I, Cel7A) were purified from the culture ultrafiltrate of a mutant strain of the fungus Chrysosporium lucknowense, an industrial producer of cellulases and hemicellulases. The enzymes had different molecular masses (52 and 65 kDa, SDS-PAGE data) but the same pI

  16. Listening to the 2011 magnitude 9.0 Tohoku-Oki, Japan, earthquake

    Science.gov (United States)

    Peng, Zhigang; Aiken, Chastity; Kilb, Debi; Shelly, David R.; Enescu, Bogdan

    2012-01-01

    The magnitude 9.0 Tohoku-Oki, Japan, earthquake on 11 March 2011 is the largest earthquake to date in Japan’s modern history and is ranked as the fourth largest earthquake in the world since 1900. This earthquake occurred within the northeast Japan subduction zone (Figure 1), where the Pacific plate is subducting beneath the Okhotsk plate at rate of ∼8–9 cm/yr (DeMets et al. 2010). This type of extremely large earthquake within a subduction zone is generally termed a “megathrust” earthquake. Strong shaking from this magnitude 9 earthquake engulfed the entire Japanese Islands, reaching a maximum acceleration ∼3 times that of gravity (3 g). Two days prior to the main event, a foreshock sequence occurred, including one earthquake of magnitude 7.2. Following the main event, numerous aftershocks occurred around the main slip region; the largest of these was magnitude 7.9. The entire foreshocks-mainshock-aftershocks sequence was well recorded by thousands of sensitive seismometers and geodetic instruments across Japan, resulting in the best-recorded megathrust earthquake in history. This devastating earthquake resulted in significant damage and high death tolls caused primarily by the associated large tsunami. This tsunami reached heights of more than 30 m, and inundation propagated inland more than 5 km from the Pacific coast, which also caused a nuclear crisis that is still affecting people’s lives in certain regions of Japan.

  17. Prevalencia de enfermedad celíaca en pacientes con hepatopatías crónicas

    OpenAIRE

    Crivelli, Adriana N.

    2016-01-01

    La enfermedad celíaca (EC) es definida como una enteropatía crónica del intestino delgado mediada por mecanismos inmunes, precipitada por la exposición al gluten y prolaminas relacionadas de la dieta, en individuos genéticamente predispuestos. Diversas enfermedades hepáticas pueden asociarse a la EC. El espectro del compromiso hepático en la EC es muy amplio e incluye, entre otros, enfermedad hepática criptogenética (desde leve a severa), hepatitis autoinmune, colangitis esclerosante primaria...

  18. Doença celíaca e infertilidade feminina: associação freqüentemente negligenciada Celiac disease and female infertility: a frequently neglected association

    Directory of Open Access Journals (Sweden)

    Carmen Lívia da Silva Martins

    2006-10-01

    Full Text Available OBJETIVO: Verificar a existência de associação entre subfertilidade ou infertilidade e concomitante presença de doença celíaca nas mulheres atendidas em ambulatório de hospital geral especializado em reprodução humana. MÉTODOS: O delineamento do estudo foi do tipo caso-controle. Os casos foram constituídos por um grupo de 200 mulheres com queixa de dificuldade para engravidar, e um grupo controle formado por 400 mulheres atendidas no mesmo ambulatório com queixas variadas, mas sem problemas de fertilidade. Ambos os grupos de pacientes foram submetidos a pesquisa de anticorpos antiendomísio pelo método de imunofluorescência indireta. Nos casos positivos, o diagnóstico foi confirmado por biópsia duodenal endoscópica e exame histopatológico. RESULTADOS: Os testes antiendomísio no grupo com dificuldade para engravidar resultaram positivos em três pacientes (1,5%. O diagnóstico de doença celíaca foi confirmado por subseqüente exame histopatológico da mucosa duodenal. Todas as pacientes pertencentes ao grupo controle apresentaram testes sorológicos negativos. CONCLUSÕES: O fato de terem sido encontrados casos de doença celíaca somente entre mulheres com queixas de dificuldade para engravidar parece um dado relevante e sugestivo de ser a doença celíaca mais comum entre mulheres inférteis; porém, há necessidade de novos estudos com tamanho de amostra maior para confirmação definitiva dessa provável associação.PURPOSE: To verify the existence of association between sub-fertility or infertility and concurrent celiac disease in women attended at a reproductive disorders out-patient clinic of a general hospital. METHODS: This was a case-control study in which a group of 200 women with difficulty in conceiving was compared with a control group with 400 women who were treated at the same hospital for various reasons, but not for fertility problems. Both groups were submitted to the anti-endomysium antibody protocol

  19. An evaluation of Admedus' tissue engineering process-treated (ADAPT) bovine pericardium patch (CardioCel) for the repair of cardiac and vascular defects.

    Science.gov (United States)

    Strange, Geoff; Brizard, Christian; Karl, Tom R; Neethling, Leon

    2015-03-01

    Tissue engineers have been seeking the 'Holy Grail' solution to calcification and cytotoxicity of implanted tissue for decades. Tissues with all of the desired qualities for surgical repair of congenital heart disease (CHD) are lacking. An anti-calcification tissue engineering process (ADAPT TEP) has been developed and applied to bovine pericardium (BP) tissue (CardioCel, AdmedusRegen Pty Ltd, Perth, WA, Australia) to eliminate cytotoxicity, improve resistance to acute and chronic inflammation, reduce calcification and facilitate controlled tissue remodeling. Clinical data in pediatric patients, and additional pre-market authorized prescriber data demonstrate that CardioCel performs extremely well in the short term and is safe and effective for a range of congenital heart deformations. These data are supported by animal studies which have shown no more than normal physiologic levels of calcification, with good durability, biocompatibility and controlled healing.

  20. Expression of two functionally distinct plant endo-beta-1,4-glucanases is essential for the compatible interaction between potato cyst nematode and its hosts.

    Science.gov (United States)

    Karczmarek, Aneta; Fudali, Sylwia; Lichocka, Malgorzata; Sobczak, Miroslaw; Kurek, Wojciech; Janakowski, Slawomir; Roosien, Jan; Golinowski, Wladyslaw; Bakker, Jaap; Goverse, Aska; Helder, Johannes

    2008-06-01

    For the proliferation of their feeding sites (syncytia), the potato cyst nematode Globodera rostochiensis is thought to recruit plant endo-beta-1,4-glucanases (EGases, EC. 3.2.1.4). Reverse-transcription polymerase chain reaction experiments on tomato (Solanum lycopersicum) indicated that the expression of two out of the at least eight EGases, namely Sl-cel7 and Sl-cel9C1, is specifically upregulated during syncytium formation. In situ hybridization and immunodetection studies demonstrated that both EGases are specifically expressed inside and adjacent to proliferating syncytia. To assess the importance of Sl-cel7 and Sl-cel9C1 for nematode development, we decided to knock them out individually. Sl-cel9C1 probably is the only class C EGase in tomato, and we were unable to regenerate Sl-cel9C1-silenced plants. Potato (S. tuberosum), a close relative of tomato, harbors at least two class C EGases, and St-cel7-or St-cel9C1-silenced potato plants showed no obvious aberrant phenotype. Infection with potato cyst nematodes resulted in a severe reduction of the number of adult females (up to 60%) and a sharp increase in the fraction of females without eggs (up to 89%). Hence, the recruitment of CEL7, an enzyme that uses xyloglucan and noncrystalline cellulose as natural substrates, and CEL9C1, an enzyme that uses crystalline cellulose, is essential for growth and development of potato cyst nematodes.

  1. Origem, ramificação e distribuição da artéria celíaca no tucano-de-bico-verde (Ramphastos dicolorus Linnaeus, 1766

    Directory of Open Access Journals (Sweden)

    Osório J. Silva Neto

    2013-03-01

    Full Text Available O tucano-de-bico-verde (Ramphastos dicolorus é uma ave encontrada nas florestas tropicais americanas e pertence à Ordem Piciforme, Família Ramphastidae. Neste trabalho objetivou-se descrever a origem, a ramificação e a distribuição da artéria celíaca do tucano-de-bico-verde. Foram utilizados três espécimes provenientes do Criatório Científico e Cultural de Poços de Caldas, MG (IBAMA, 2.31.94-00006, doados após óbito por causas naturais. As aves tiveram a artéria isquiática direita canulada para injeção de solução de látex corado, e após fixação em solução de formol a 10% foram dissecadas. A artéria celíaca originou-se a partir da porção descendente da aorta, emitindo como primeiro ramo colateral a artéria pró-ventricular dorsal. Esta emitiu ramos esofágicos e continuou-se como artéria gástrica dorsal, de aspecto tortuoso, terminando em anastomose com a artéria gástrica direita. Após curto trajeto, a artéria celíaca formou dois ramos colaterais, o esquerdo e o direito. O ramo esquerdo logo se ramificou formando a artéria pró-ventricular ventral com seus ramos esofágicos, artéria gástrica esquerda, que originou a artéria hepática esquerda, e finalmente a artéria gastroduodenal, que emitiu as artérias gástricas ventrais e duodenais. O ramo direito da artéria celíaca emitiu as artérias lienais e hepática direita, continuando-se como artéria pancreático-duodenal. Esta formou a artéria pilórica dorsal, duas artérias gástricas direitas, vários ramos duodenais, pancreáticos e a artéria duodeno-jejunal. Assim, a artéria celíaca nos três espécimes de tucano-de-bico-verde, exibiu um arranjo que se assemelha tanto ao descrito em aves domésticas quanto ao de aves silvestres.

  2. Novel Technique to Overcome the Nonavailability of a Long Needle 9-0 Polypropylene Suture for Sutured Scleral Fixation of the Posterior Chamber Intraocular Lens Using a Single Fisherman's Knot.

    Science.gov (United States)

    Shin, Yong Un; Seong, Mincheol; Cho, Hee Yoon; Kang, Min Ho

    2017-01-01

    To describe a method to overcome the nonavailability of a long needle 9-0 polypropylene suture for sutured scleral fixation of the posterior chamber intraocular lens (PC-IOL) using a single fisherman's knot (SFK). First, a 10-0 polypropylene suture was passed from the sclera to the ciliary sulcus using a long needle. A 9-0 suture was tied to the unpassed portion of the 10-0 suture with an SFK. We pulled the 10-0 suture to pass the SFK through the sclera, and then we cut the knot and removed the 10-0 suture. IOL fixation with 9-0 sutures used the conventional techniques used for sutured scleral-fixated IOL. Preoperative and postoperative visual acuity, corneal astigmatism, and endothelial cell count and intraoperative/postoperative complications were evaluated. An SFK joining the two sutures was passed through the sclera without breakage or slippage. A total of 35 eyes from 35 patients who underwent sutured scleral fixation of the IOL. An intraoperative complication (minor intraocular hemorrhage) was recorded in four cases. Knot exposure, IOL dislocation, subluxation, and retinal detachment were not observed. The SFK offers the opportunity to use 9-0 sutures for the long-term safety and may not require the surgeon to learn any new technique.

  3. Bacterial Community Structure and Biochemical Changes Associated With Composting of Lignocellulosic Oil Palm Empty Fruit Bunch

    Directory of Open Access Journals (Sweden)

    Mohd Huzairi Mohd Zainudin

    2013-11-01

    Full Text Available Bacterial community structure and biochemical changes during the composting of lignocellulosic oil palm empty bunch (EFB and palm oil mill effluent (POME anaerobic sludge were studied by examining the succession of the bacterial community and its association with changes in lignocellulosic components by denaturing gradient gel electrophoresis (DGGE and the 16S rRNA gene clone library. During composting, a major reduction in cellulose after 10 days from 50% to 19% and the carbon content from 44% to 27% towards the end of the 40-day composting period were observed. The C/N ratio also decreased. A drastic change in the bacterial community structure and diversity throughout the composting process was clearly observed using PCR-DGGE banding patterns. The bacterial community drastically shifted between the thermophilic and maturing stages. 16s rRNA clones belonging to the genera Bacillus, Exiguobacterium, Desemzia, and Planococcus were the dominant groups throughout composting. The species closely related to Solibacillus silvestris were found to be major contributors to changes in the lignocellulosic component. Clones identified as Thermobacillus xylanilyticus, Brachybacterium faecium, Cellulosimicrobium cellulans, Cellulomonas sp., and Thermobifida fusca, which are known to be lignocellulosic-degrading bacteria, were also detected and are believed to support the lignocellulose degradation.

  4. La Corte Europea de Derechos Humanos y la protección de la autonomía de las comunidades religiosas: análisis del caso Sindicatul Pãstorul cel Bun con Rumania

    Directory of Open Access Journals (Sweden)

    Stéphanie Wattier

    2014-06-01

    Full Text Available El presente artículo analiza la sentencia dictada por la Gran Sala de la Corte Europea de Derechos Humanos, en el caso Sindicatul Pãstorul cel Bun con Rumania de 9 de julio de 2013. La autora examina el razonamiento de la Corte Europea y describe la forma en que se ha concebido la relación entre la libertad religiosa y la libertad de asociación en la jurisprudencia del Tribunal de Estrasburgo. A partir de este análisis, concluye que actualmente no existe un modelo europeo de relaciones entre el Estado y las confesiones religiosas, lo que ha llevado a la Corte Europea a aplicar con mayor laxitud el margen de apreciación nacional.

  5. Doença celíaca: sua relação com a saúde bucal Celiac disease's relationship with the oral health

    Directory of Open Access Journals (Sweden)

    Michelle Soares Rauen

    2005-04-01

    Full Text Available Doença Celíaca é uma intolerância permanente às proteínas contidas no glúten de alguns cereais, como o trigo, o centeio, a cevada e a aveia. A doença manifesta-se principalmente nos primeiros dois anos de vida, sendo o intestino delgado o principal órgão afetado, com manifestações clínicas de diarréia, vômitos e emagrecimento; porém, o diagnóstico, muitas vezes, é difícil, devido ao grande número de casos atípicos da doença. Nestes casos, os sintomas podem ser numerosos e diversificados, tais como baixa estatura, anemia, osteoporose, hipoplasia do esmalte dentário, além de sintomas próprios do quadro clínico de outras doenças imunológicas que podem associar-se à doença celíaca, tais como diabetes mellitus, dermatite herpertiforme, doenças da tireóide, alergia, estomatite aftosa recorrente, entre outras. Devido a essa associação, os profissionais da saúde procurados pelos pacientes podem não relacionar os sintomas à enteropatia; entretanto, esta, se não tratada, pode trazer várias outras complicações à saúde. O objetivo desta comunicação é demonstrar a importância das manifestações bucais, as quais, quando devidamente observadas, contribuem ao diagnóstico da doença celíaca.Celiac Disease is a permanent intolerance to proteins contained in the gluten of some cereals, such as wheat, rye, barley and oat. The disease appears mainly during the first two years of life, the small bowel being the main affected organ, with clinical manifestations such as diarrhea, vomiting and weight loss. The diagnosis, however, is often difficult, due to the large number of atypical manifestations of the disease. In such cases, numerous and diversified symptoms, such as low stature, anemia, osteoporosis, and dental enamel hypoplasia, may be concurrent with symptoms of immune diseases associated to the celiac disease (diabetes mellitus, dermatitis herpetiformis, thyroid diseases, allergy, and recurrent aphtous

  6. Characterization of CYP154F1 from Thermobifida fusca YX and Extension of Its Substrate Spectrum by Site-Directed Mutagenesis.

    Science.gov (United States)

    Rühlmann, Ansgar; Groth, Georg; Urlacher, Vlada B

    2018-03-02

    Previous studies on cytochrome P450 monooxygenases (CYP) from family 154 reported their substrate promiscuity and high activity. Hence, herein, the uncharacterized family member CYP154F1 is described. Screening of more than 100 organic compounds revealed that CYP154F1 preferably accepts small linear molecules with a carbon chain length of 8-10 atoms. In contrast to thoroughly characterized CYP154E1, CYP154F1 has a much narrower substrate spectrum and lower activity. A structural alignment of homology models of CYP154F1 and CYP154E1 revealed few differences in the active sites of both family members. By gradual mutagenesis of the CYP154F1 active site towards those of CYP154E1, a key residue accounting for the different activities of both enzymes was identified at position 234. Substitution of T234 for large hydrophobic amino acids led to up to tenfold higher conversion rates of small substrates, such as geraniol. Replacement of T234 by small hydrophobic amino acids, valine or alanine, resulted in mutants with extended substrate spectra. These mutants are able to convert some of the larger substrates of CYP154E1, such as (E)-stilbene and (+)-nootkatone. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Biotransformation of mercury in pH-stat cultures of eukaryotic freshwater algae.

    Science.gov (United States)

    Kelly, David J A; Budd, Kenneth; Lefebvre, Daniel D

    2007-01-01

    Eukaryotic algae were studied to determine their ability to biotransform Hg(II) under aerated and pH controlled conditions. All algae converted Hg(II) into beta-HgS and Hg(0) to varying degrees. When Hg(II) was administered as HgCl(2) to the algae, biotransformation by species of Chlorophyceae (Selenastrum minutum and Chlorella fusca var. fusca) was initiated with beta-HgS synthesis (K (1/2) of hours) and concomitant Hg degrees evolution occurred in the first hour. Hg degrees synthesis was impeded by the formation of beta-HgS and this inhibition was released in C. fusca var. fusca when cellular thiols were oxidized by the addition of dimethylfumarate (DMF). The diatom, Navicula pelliculosa (Bacillariophyceae), converted a substantially greater proportion of the applied Hg(II) into Hg(0), whereas the thermophilic alga, Galdieria sulphuraria (Cyanidiophyceae), rapidly biotransformed as much as 90% of applied Hg(II) into beta-HgS (K (1/2) approximately 20 min). This thermophile was also able to generate Hg(0) even after all exogenously applied HgCl(2) had been biotransformed. The results suggest that beta-HgS may be the major dietary mercurial for grazers of contaminated eukaryotic algae.

  8. Novel Technique to Overcome the Nonavailability of a Long Needle 9-0 Polypropylene Suture for Sutured Scleral Fixation of the Posterior Chamber Intraocular Lens Using a Single Fisherman’s Knot

    Directory of Open Access Journals (Sweden)

    Yong Un Shin

    2017-01-01

    Full Text Available Purpose. To describe a method to overcome the nonavailability of a long needle 9-0 polypropylene suture for sutured scleral fixation of the posterior chamber intraocular lens (PC-IOL using a single fisherman’s knot (SFK. Methods. First, a 10-0 polypropylene suture was passed from the sclera to the ciliary sulcus using a long needle. A 9-0 suture was tied to the unpassed portion of the 10-0 suture with an SFK. We pulled the 10-0 suture to pass the SFK through the sclera, and then we cut the knot and removed the 10-0 suture. IOL fixation with 9-0 sutures used the conventional techniques used for sutured scleral-fixated IOL. Preoperative and postoperative visual acuity, corneal astigmatism, and endothelial cell count and intraoperative/postoperative complications were evaluated. Results. An SFK joining the two sutures was passed through the sclera without breakage or slippage. A total of 35 eyes from 35 patients who underwent sutured scleral fixation of the IOL. An intraoperative complication (minor intraocular hemorrhage was recorded in four cases. Knot exposure, IOL dislocation, subluxation, and retinal detachment were not observed. Conclusions. The SFK offers the opportunity to use 9-0 sutures for the long-term safety and may not require the surgeon to learn any new technique.

  9. Celiac disease onset after pegylated interferon and ribavirin treatment of chronic hepatitis C Doença celíaca após tratamento de hepatite C crônica com interferon peguilado e ribavirina

    Directory of Open Access Journals (Sweden)

    Elson V. Martins Jr.

    2004-06-01

    Full Text Available AIM: Report of a case of a woman patient who developed celiac disease after pegylated interferon alpha-2a and ribavirin use for chronic hepatitis C. PATIENT AND METHOD: A 34-year-old woman with chronic hepatitis C, genotype 3, receiving pegylated interferon alpha-2a and ribavirin for 6 months, developed progressive malaise and anemia 6 months after the end of treatment. RESULT: Additional investigation revealed duodenal villous atrophy and positivity for anti-endomysium and anti-gliadin antibodies. Celiac disease diagnosis was performed and symptoms and laboratory abnormalities improved after gluten-free diet. CONCLUSION: Celiac disease must be ruled out in patients with malabsorption complaints in or after interferon (or pegylated interferon therapy. Screening for celiac disease with detection of anti-endomysium antibodies would be done in susceptible patients.OBJETIVO: Relatar caso de doença celíaca ocorrendo após uso de interferon peguilado e ribavirina em paciente com hepatite C crônica. PACIENTE E MÉTODO: Mulher de 34 anos com hepatite C crônica, genótipo 3, tratada com interferon peguilado alfa-2a e ribavirina durante 6 meses, desenvolveu quadro de astenia e anemia após 6 meses do término do tratamento. RESULTADO: Investigação complementar revelou atrofia vilositária à biopsia duodenal e detecção de anticorpos anti-endomísio e anti-gliadina, realizando-se diagnóstico de doença celíaca. Dieta isenta de glúten foi instituída, observando-se boa resposta clínica e laboratorial. CONCLUSÃO: Doença celíaca deve ser afastada em pacientes com quadro de má absorção durante ou após uso de interferon (ou interferon peguilado. Rastreamento de doença celíaca através da realização de anticorpo anti-endomísio pode ser considerado em populações susceptíveis.

  10. Grado de información, acceso y prácticasde elaboración de alimentos sin TACC de beneficios del programa de Asistencia Alimentaria para Celíacos de General Madariaga

    OpenAIRE

    Chaparro, María Victoria

    2015-01-01

    La Enfermedad Celíaca, también conocida como esprúe celíaco o enteropatía sensible al gluten, se la define como la intolerancia alimentaria de orden genético más frecuente de la especie humana. El único tratamiento es una dieta estricta y de por vida Sin TACC. La incidencia es mayor en mujeres, que en varones, siendo su prevalencia aproximadamente el 1% de la población. Objetivos: Determinar el grado de información, el acceso y las prácticas de elaboración de alimentos Sin T...

  11. Biochemical and mutational analyses of a multidomain cellulase/mannanase from Caldicellulosiruptor bescii.

    Science.gov (United States)

    Su, Xiaoyun; Mackie, Roderick I; Cann, Isaac K O

    2012-04-01

    Thermophilic cellulases and hemicellulases are of significant interest to the biofuel industry due to their perceived advantages over their mesophilic counterparts. We describe here biochemical and mutational analyses of Caldicellulosiruptor bescii Cel9B/Man5A (CbCel9B/Man5A), a highly thermophilic enzyme. As one of the highly secreted proteins of C. bescii, the enzyme is likely to be critical to nutrient acquisition by the bacterium. CbCel9B/Man5A is a modular protein composed of three carbohydrate-binding modules flanked at the N terminus and the C terminus by a glycoside hydrolase family 9 (GH9) module and a GH5 module, respectively. Based on truncational analysis of the polypeptide, the cellulase and mannanase activities within CbCel9B/Man5A were assigned to the N- and C-terminal modules, respectively. CbCel9B/Man5A and its truncational mutants, in general, exhibited a pH optimum of ∼5.5 and a temperature optimum of 85°C. However, at this temperature, thermostability was very low. After 24 h of incubation at 75°C, the wild-type protein maintained 43% activity, whereas a truncated mutant, TM1, maintained 75% activity. The catalytic efficiency with phosphoric acid swollen cellulose as a substrate for the wild-type protein was 7.2 s(-1) ml/mg, and deleting the GH5 module led to a mutant (TM1) with a 2-fold increase in this kinetic parameter. Deletion of the GH9 module also increased the apparent k(cat) of the truncated mutant TM5 on several mannan-based substrates; however, a concomitant increase in the K(m) led to a decrease in the catalytic efficiencies on all substrates. These observations lead us to postulate that the two catalytic activities are coupled in the polypeptide.

  12. Heat Transfer Experiments with Supercritical CO{sub 2} in a Vertical Circular Tube (9.0 mm)

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Tae Ho; Kim, Hwan Yeol [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Sim, Woo Gun; Bae, Yoon Yeong [Hannam University, Daejeon (Korea, Republic of)

    2008-10-15

    Heat transfer test facility, SPHINX(Supercritical Pressure Heat transfer Investigation for NeXt generation), has been operated at KAERI for an investigation of the thermal-hydraulic behaviors of supercritical CO{sub 2} at several test sections with a different geometry. The loop uses CO{sub 2} because it has critical pressure and temperature which is much lower than water. Experimental study of heat transfer to supercritical CO{sub 2} in a vertical circular tube with and inner diameter of 9.0mm has been performed. CO{sub 2} flows downward through the vertical circular tube for the simulation of the water rod which may be used for a moderation of the reactor. The heat transfer characteristics were analyzed and compared with the upward flow test results previously performed at the same test section at KAERI.

  13. Cardiorespiratory fitness: relationship with the immune system of adolescents

    Directory of Open Access Journals (Sweden)

    Anderson Zampier Ulbrich

    2009-01-01

    Full Text Available http://dx.doi.org/10.5007/1980-0037.2009v11n4p478 This work to investigate the relationship between cardiorespiratory fitness with count of cells in the immune system (IS in adolescents. The sample was composed by 102 boys (B and 131 (G girls (12 to 17 years-old. BMI was calculated and categorized (Cole et al. and absolute and relative VO2máx was obtained through the shuttle run test (Léger et al. and categorized according to Rodrigues et al. Maturation stage was assessing (Tanner. The cells of IS by leukogram was obtained. It was hypothesized (H that individuals with higher VO2máx could present a higher ratio of chances in the counting of cells in the SI. Was used frequency distribution, Student t test, ANOVAs one-way and binary logistic regression (p< 0.05. The overweight was demonstrated that 27.8% (B 13.2% (G. The values of VO2máx were between 29 to 59 ml.kg-1.min-1 and 25 to 53 ml.kg-1.min-1 to B and G, respectively. The average of IS cells differ between B and G, and in greater range in the: Leukocytes (B = 5697.1 cel.ml and G = 6496.9 cel.ml; t= 3,959 p = 0,000, Segmented (B= 3288,1 cel.ml and G= 4023,8 cel.ml; t= 4,145; p= 0,000, and neutrophils (B = 3306,9 cel.ml and G = 4101.2 cel.ml; t= 4,431 , p= 0,000 in G, while Eosinophils (B = 286.1 cel.ml and G = 211.1 cel.ml; t= 2,644; p= 0,009 had a higher count for B. There were differences between the maturational stages only between puberty stage and pos puberty for Leukocytes and Segmented in G. The analyses of relative VO2máx indicated the M with low cardiorespiratory fitness is 4,6; 2,76; 3,57 respectively have more chances of eosinophils, lymphocytes and monocytes below the 50th percentile, as well as to observe the absolute VO2máx, the R with regular fitness is 9,0; 5,16; 9,65 respectively have more chances of leukocytes, monocytes and neutrophils below the 50th percentile. The M showed that the regular fitness has 3.0 times more chances to have leukocytes and neutrophils below

  14. ICECO-CEL: a coupled Eulerian-Lagrangian code for analyzing primary system response in fast reactors

    International Nuclear Information System (INIS)

    Wang, C.Y.

    1981-02-01

    This report describes a coupled Eulerian-Lagrangian code, ICECO-CEL, for analyzing the response of the primary system during hypothetical core disruptive accidents. The implicit Eulerian method is used to calculate the fluid motion so that large fluid distortion, two-dimensional sliding interface, flow around corners, flow through coolant passageways, and out-flow boundary conditions can be treated. The explicit Lagrangian formulation is employed to compute the response of the containment vessel and other elastic-plastic solids inside the reactor containment. Large displacements, as well as geometrical and material nonlinearities are considered in the analysis. Marker particles are utilized to define the free surface or the material interface and to visualize the fluid motion. The basic equations and numerical techniques used in the Eulerian hydrodynamics and Lagrangian structural dynamics are described. Treatment of the above-core hydrodynamics, sodium spillage, fluid cavitation, free-surface boundary conditions and heat transfer are also presented. Examples are given to illustrate the capabilities of the computer code. Comparisons of the code predictions with available experimental data are also made

  15. Discordância de apresentação da doença celíaca em gêmeos monozigóticos

    Directory of Open Access Journals (Sweden)

    Magda Bahia

    2010-03-01

    Full Text Available CONTEXTO: A doença celíaca é uma enteropatia autoimune causada pela sensibilidade ao glúten em indivíduos geneticamente predispostos. Apesar da característica genética da doença, estudos demonstram discordância de 30% na sua apresentação em gêmeos monozigóticos. OBJETIVO: Apresentar dois pares de gêmeos monozigóticos, comprovados por estudos genéticos, discordantes para apresentação da doença celíaca. MÉTODO: Os pacientes foram acompanhados no Serviço de Gastroenterologia Pediátrica do Hospital das Clínicas da Universidade Federal de Minas Gerais desde 1990, sendo submetidos a exames clínicos periódicos, biopsias intestinais e sorologia para anticorpos IgG e IgA antigliadina, determinados pela técnica de ELISA (ensaio imunoenzimático, e anticorpos classe IgA antiendomísio, determinados pela técnica de imunofluorescência indireta. Estudos genéticos foram realizados através da técnica de amplificação por PCR e posterior tipagem de loci de microssatélites do tipo STR (short tandem repeats. RESULTADOS: Em cada par de gêmeos, apenas um apresentou doença celíaca até o momento, mostrando que, apesar do genótipo idêntico, este não foi o único determinante para a expressão da doença. CONCLUSÃO: Outros fatores, ambientais e genéticos, parecem contribuir para determinação da doença.CONTEXT: The celiac disease is an immune-mediated enteropathy caused by a permanent sensitivity to gluten in genetically susceptible individuals. Despite the genetic characteristic of the disease, studies show discrepancy of 30% in its presentation in monozygotic twins. OBJECTIVE: To present two pairs of monozygotic confirmed by genetic study and discordant for presentation of celiac disease. METHODS: The patients were followed up at the Pediatric Gastroenterology Service - Hospital das Clínicas da Universidade Federal de Minas Gerais, MG, Brazil, since 1990, and were submitted to periodical clinical examinations, intestinal

  16. Modified wick method using Weck-Cel sponges for collection of human rectal secretions and analysis of mucosal HIV antibody.

    Science.gov (United States)

    Kozlowski, P A; Lynch, R M; Patterson, R R; Cu-Uvin, S; Flanigan, T P; Neutra, M R

    2000-08-01

    Weck-Cel sponges were examined for suitability as an absorbent material for nontraumatic collection of rectal secretions in humans. Sponges were tested in vitro and determined by quantitative enzyme-linked immunosorbent assay (ELISA) to be capable of releasing 100% of absorbed albumin and all immunoglobulin subtypes after treatment with detergent-supplemented buffer. Protein composition in rectal secretions collected from normal women with dry sponges (DS) or with sponges previously softened by moistening with saline (MS) was subsequently compared. DS secretions showed evidence of contamination with blood and interstitial fluid-derived albumin, immunoglobulin G (IgG), and monomeric IgA. MS secretions appeared to represent local mucosal secretions more accurately because they contained negligible blood, a greater percentage of secretory IgA within the total IgA, and both lower albumin/IgG ratios and more dramatic alterations in IgG subclass distribution compared with corresponding serum. Anti-HIV IgG, IgM, IgA, and antibodies with secretory component could be demonstrated by ELISA in rectal secretions collected with moist sponges from 8 of 8, 1 of 8, 5 of 8, and 3 of 8 HIV-infected women, respectively. The data show that Weck-Cel sponges, if premoistened, can be used to collect rectal fluids nontraumatically and to obtain quantitative information about concentrations of immunoglobulins and specific antibodies on rectal mucosal surfaces.

  17. Baseline hematology and serum biochemistry results for Indian leopards (Panthera pardus fusca

    Directory of Open Access Journals (Sweden)

    Arun Attur Shanmugam

    2017-07-01

    Full Text Available Aim: The aim of the study was to establish the baseline hematology and serum biochemistry values for Indian leopards (Panthera pardus fusca, and to assess the possible variations in these parameters based on age and gender. Materials and Methods: Hemato-biochemical test reports from a total of 83 healthy leopards, carried out as part of routine health evaluation in Bannerghatta Biological Park and Manikdoh Leopard Rescue Center, were used to establish baseline hematology and serum biochemistry parameters for the subspecies. The hematological parameters considered for the analysis included hemoglobin (Hb, packed cell volume, total erythrocyte count (TEC, total leukocyte count (TLC, mean corpuscular volume (MCV, mean corpuscular Hb (MCH, and MCH concentration. The serum biochemistry parameters considered included total protein (TP, albumin, globulin, aspartate aminotransferase, alanine aminotransferase (ALT, blood urea nitrogen, creatinine, triglycerides, calcium, and phosphorus. Results: Even though few differences were observed in hematologic and biochemistry values between male and female Indian leopards, the differences were statistically not significant. Effects of age, however, were evident in relation to many hematologic and biochemical parameters. Sub-adults had significantly greater values for Hb, TEC, and TLC compared to adults and geriatric group, whereas they had significantly lower MCV and MCH compared to adults and geriatric group. Among, serum biochemistry parameters the sub-adult age group was observed to have significantly lower values for TP and ALT than adult and geriatric leopards. Conclusion: The study provides a comprehensive analysis of hematologic and biochemical parameters for Indian leopards. Baselines established here will permit better captive management of the subspecies, serve as a guide to assess the health and physiological status of the free ranging leopards, and may contribute valuable information for making

  18. Baseline hematology and serum biochemistry results for Indian leopards (Panthera pardus fusca)

    Science.gov (United States)

    Shanmugam, Arun Attur; Muliya, Sanath Krishna; Deshmukh, Ajay; Suresh, Sujay; Nath, Anukul; Kalaignan, Pa; Venkataravanappa, Manjunath; Jose, Lyju

    2017-01-01

    Aim: The aim of the study was to establish the baseline hematology and serum biochemistry values for Indian leopards (Panthera pardus fusca), and to assess the possible variations in these parameters based on age and gender. Materials and Methods: Hemato-biochemical test reports from a total of 83 healthy leopards, carried out as part of routine health evaluation in Bannerghatta Biological Park and Manikdoh Leopard Rescue Center, were used to establish baseline hematology and serum biochemistry parameters for the subspecies. The hematological parameters considered for the analysis included hemoglobin (Hb), packed cell volume, total erythrocyte count (TEC), total leukocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), and MCH concentration. The serum biochemistry parameters considered included total protein (TP), albumin, globulin, aspartate aminotransferase, alanine aminotransferase (ALT), blood urea nitrogen, creatinine, triglycerides, calcium, and phosphorus. Results: Even though few differences were observed in hematologic and biochemistry values between male and female Indian leopards, the differences were statistically not significant. Effects of age, however, were evident in relation to many hematologic and biochemical parameters. Sub-adults had significantly greater values for Hb, TEC, and TLC compared to adults and geriatric group, whereas they had significantly lower MCV and MCH compared to adults and geriatric group. Among, serum biochemistry parameters the sub-adult age group was observed to have significantly lower values for TP and ALT than adult and geriatric leopards. Conclusion: The study provides a comprehensive analysis of hematologic and biochemical parameters for Indian leopards. Baselines established here will permit better captive management of the subspecies, serve as a guide to assess the health and physiological status of the free ranging leopards, and may contribute valuable information for making effective

  19. Complexation studies with 90Y from a novel 90Sr-90Y generator

    International Nuclear Information System (INIS)

    Venkatesh, M.; Pandey, U.; Banerjee, S.; Samuel, G.; Pillai, M.R.A.; Dhami, P.S.; Kannan, R.; Achuthan, P.V.; Chitnis, R.R.; Gopalakrishnan, V.; Ramanujam, A.

    2001-01-01

    Some features of a novel 90 Sr- 90 Y generator which employs supported liquid membrane (SLM) to separate carrier-free 90 Y from 90 Sr present in the high level waste of the spent fuel of reactor are described. After ascertaining the purity of 90 Y particularly with respect to 90 Sr breakthrough, its complexation was studied with a few oxo/aza donor ligands, such as DTPA, EDTMP, DOTA, TETA and a cyclic phosphonate, CTMP. These studies were primarily carried out to adjudge the quality of the 90 Y obtained from a novel 90 Sr- 90 Y generator and ascertain its usability for labelling biomolecules such as antibodies and peptides. The DOTA complexes are most stable at 37 C in human serum; they appear to be ideal bifunctional chelating agent for use in radioimmunotherapy with 90 Y. (orig.)

  20. Insertion of an active fixation lead in the inferior interatrial septum via a 9.0 Fr guiding catheter

    Directory of Open Access Journals (Sweden)

    Shumpei Mori, MD

    2014-04-01

    Full Text Available Placing an atrial lead in the inferior interatrial septum (IAS reportedly reduces the incidence of paroxysmal atrial fibrillation (AF and slows the progression to chronic AF; however, in certain cases, inferior IAS pacing is technically difficult. When this procedure is unsuccessful, insertion of the lead in the right atrial appendage can be considered, but it is associated with a risk of cardiac perforation. Here, we describe a technique for lead insertion in the inferior IAS via a 9.0 Fr guiding catheter, which may serve as an alternative technique for inferior IAS pacing when the conventional stylet-guided insertion is not successful.

  1. Fabrication of polystyrene/agave particle biocomposites using ...

    Indian Academy of Sciences (India)

    marine, sports, automobile, aerospace industry, etc. This is mainly due to increasing environmental concerns and ..... -4.0. -6.0. -8.0. -10.0. 391 Cel. 1.84 mg/min. 22 Cel. 100.0 %. 101 Cel. 100.2 %. 419 Cel. 1.1 %. 965 Ce. -0.8 %. 275 Cel. 97.9 %. 486 Cel. 14.47 uV. 395 Cel. 4.82 uV. -172 mJ/mg. -150 mJ/mg. 121 mJ/mg.

  2. Doença celíaca em tratamento: avaliação da densidade mineral óssea Celiac disease under treatment: evaluation of bone mineral density

    Directory of Open Access Journals (Sweden)

    Cecília Noronha de Miranda Carvalho

    2003-08-01

    Full Text Available OBJETIVO: comparar a densidade mineral óssea de crianças e adolescentes com doença celíaca em tratamento com controles sadios, e avaliar exames laboratoriais relacionados com o metabolismo do cálcio. MÉTODOS: foram estudados 30 pacientes com doença celíaca em dieta isenta de glúten, 17 crianças e 13 adolescentes, e 23 indivíduos saudáveis. Todos os pacientes e controles realizaram a densidade mineral óssea (DEXA, Lunar. Os pacientes realizaram dosagem sérica de cálcio total, cálcio ionizado, fósforo, magnésio, fosfatase alcalina e paratormônio. RESULTADOS: a média de peso, estatura e densidade mineral óssea dos adolescentes com doença celíaca foi menor do que dos controles (pOBJECTIVE: the present study was designed to compare the bone mineral density of children and adolescents with celiac disease to the bone mineral density of controls, and to evaluate laboratory analysis of calcium metabolism of celiac disease patients. METHODS: thirty celiac disease patients (17 children, 13 adolescents, on a gluten-free diet, and 23 healthy subjects were studied. Tests of bone mineral density of the lumbar spine (DEXA, Lunar were performed in all patients and controls. Laboratory analysis of calcium metabolism was performed in all patients. RESULTS: mean weight and height of adolescents with celiac disease were lower than mean weight and height of controls (p<0.05. Bone mineral density in adolescents with celiac disease was significantly reduced if compared to controls (p=0.015, whereas no significant difference was found among children with celiac disease and controls. The number of adolescents who had started a gluten-free diet after the age of 2 years was higher than in children (p=0.003. Serum levels of ionized calcium, total calcium and parathormone were normal. CONCLUSIONS: the one mineral density of adolescents with celiac disease was lower than controls; whereas, no difference was found between the bone mineral density of

  3. Neutron Reflectometry and QCM-D Study of the Interaction of Cellulase Enzymes with Films of Amorphous Cellulose

    International Nuclear Information System (INIS)

    Halbert, Candice E.; Ankner, John Francis; Kent, Michael S.; Jaclyn, Murton K.; Browning, Jim; Cheng, Gang; Liu, Zelin; Majewski, Jaroslaw; Supratim, Datta; Michael, Jablin; Bulent, Akgun; Alan, Esker; Simmons, Blake

    2011-01-01

    Improving the efficiency of enzymatic hydrolysis of cellulose is one of the key technological hurdles to reduce the cost of producing ethanol and other transportation fuels from lignocellulosic material. A better understanding of how soluble enzymes interact with insoluble cellulose will aid in the design of more efficient enzyme systems. We report a study involving neutron reflectometry (NR) and quartz crystal microbalance with dissipation (QCM-D) of the interaction of a commercial fungal enzyme extract (T. viride), two purified endoglucanses from thermophilic bacteria (Cel9A from A. acidocaldarius and Cel5A from T. maritima), and a mesophilic fungal endoglucanase (Cel45A from H. insolens) with amorphous cellulose films. The use of amorphous cellulose is motivated by the promise of ionic liquid pretreatment as a second generation technology that disrupts the native crystalline structure of cellulose. NR reveals the profile of water through the film at nm resolution, while QCM-D provides changes in mass and film stiffness. At 20 C and 0.3 mg/ml, the T. viride cocktail rapidly digested the entire film, beginning from the surface followed by activity throughout the bulk of the film. For similar conditions, Cel9A and Cel5A were active for only a short period of time and only at the surface of the film, with Cel9A releasing 40 from the ∼ 700 film and Cel5A resulting in only a slight roughening/swelling effect at the surface. Subsequent elevation of the temperature to the Topt in each case resulted in a very limited increase in activity, corresponding to the loss of an additional 60 from the film for Cel9A and 20 from the film for Cel5A, and very weak penetration into and digestion within the bulk of the film, before the activity again ceased. The results for Cel9A and Cel5A contrast sharply with results for Cel45A where very rapid and extensive penetration and digestion within the bulk of the film was observed at 20 C. We speculate that the large differences are due

  4. A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production.

    Science.gov (United States)

    Li, Chengcheng; Lin, Fengming; Li, Yizhen; Wei, Wei; Wang, Hongyin; Qin, Lei; Zhou, Zhihua; Li, Bingzhi; Wu, Fugen; Chen, Zhan

    2016-09-01

    The conversion of cellulose by cellulase to fermentable sugars for biomass-based products such as cellulosic biofuels, biobased fine chemicals and medicines is an environment-friendly and sustainable process, making wastes profitable and bringing economic benefits. Trichoderma reesei is the well-known major workhorse for cellulase production in industry, but the low β-glucosidase activity in T. reesei cellulase leads to inefficiency in biomass degradation and limits its industrial application. Thus, there are ongoing interests in research to develop methods to overcome this insufficiency. Moreover, although β-glucosidases have been demonstrated to influence cellulase production and participate in the regulation of cellulase production, the underlying mechanism remains unclear. The T. reesei recombinant strain TRB1 was constructed from T. reesei RUT-C30 by the T-DNA-based mutagenesis. Compared to RUT-C30, TRB1 displays a significant enhancement of extracellular β-glucosidase (BGL1) activity with 17-fold increase, a moderate increase of both the endoglucanase (EG) activity and the exoglucanase (CBH) activity, a minor improvement of the total filter paper activity, and a faster cellulase induction. This superiority of TRB1 over RUT-C30 is independent on carbon sources and improves the saccharification ability of TRB1 cellulase on pretreated corn stover. Furthermore, TRB1 shows better resistance to carbon catabolite repression than RUT-C30. Secretome characterization of TRB1 shows that the amount of CBH, EG and BGL in the supernatant of T. reesei TRB1 was indeed increased along with the enhanced activities of these three enzymes. Surprisingly, qRT-PCR and gene cloning showed that in TRB1 β-glucosidase cel3D was mutated through the random insertion by AMT and was not expressed. The T. reesei recombinant strain TRB1 constructed in this study is more desirable for industrial application than the parental strain RUT-C30, showing extracellular β-glucosidase hyper

  5. Bioactivation of the heterocyclic aromatic amine 2-amino-3-methyl-9H-pyrido [2,3-b]indole (MeA alpha C) in recombinant test systems expressing human xenobiotic-metabolizing enzymes

    DEFF Research Database (Denmark)

    Glatt, H.; Pabel, U.; Meinl, W.

    2004-01-01

    2-Amino-3-methyl-9H-pyrido[2,3-b]indole (MeAalphaC) and some metabolites were investigated for mutagenicity in mammalian cell lines and bacterial strains engineered for the expression of human enzymes. MeAalphaC induced gene mutations (studied at the hprt locus) in Chinese hamster V79-derived cel...

  6. Araloside C Prevents Hypoxia/Reoxygenation-Induced Endoplasmic Reticulum Stress via Increasing Heat Shock Protein 90 in H9c2 Cardiomyocytes

    Directory of Open Access Journals (Sweden)

    Yuyang Du

    2018-04-01

    Full Text Available Araloside C (AsC is a cardioprotective triterpenoid compound that is mainly isolated from Aralia elata. This study aims to determine the effects of AsC on hypoxia-reoxygenation (H/R-induced apoptosis in H9c2 cardiomyocytes and its underlying mechanisms. Results demonstrated that pretreatment with AsC (12.5 μM for 12 h significantly suppressed the H/R injury in H9c2 cardiomyocytes, including improving cell viability, attenuating the LDH leakage and preventing cardiomyocyte apoptosis. AsC also inhibited H/R-induced ER stress by reducing the activation of ER stress pathways (PERK/eIF2α and ATF6, and decreasing the expression of ER stress-related apoptotic proteins (CHOP and caspase-12. Moreover, AsC greatly improved the expression level of HSP90 compared with that in the H/R group. The use of HSP90 inhibitor 17-AAG and HSP90 siRNA blocked the above suppression effect of AsC on ER stress-related apoptosis caused by H/R. Taken together, AsC could reduce H/R-induced apoptosis possibly because it attenuates ER stress-dependent apoptotic pathways by increasing HSP90 expression.

  7. The role of the Arabidopsis FUSCA3 transcription factor during inhibition of seed germination at high temperature.

    Science.gov (United States)

    Chiu, Rex S; Nahal, Hardeep; Provart, Nicholas J; Gazzarrini, Sonia

    2012-01-27

    Imbibed seeds integrate environmental and endogenous signals to break dormancy and initiate growth under optimal conditions. Seed maturation plays an important role in determining the survival of germinating seeds, for example one of the roles of dormancy is to stagger germination to prevent mass growth under suboptimal conditions. The B3-domain transcription factor FUSCA3 (FUS3) is a master regulator of seed development and an important node in hormonal interaction networks in Arabidopsis thaliana. Its function has been mainly characterized during embryonic development, where FUS3 is highly expressed to promote seed maturation and dormancy by regulating ABA/GA levels. In this study, we present evidence for a role of FUS3 in delaying seed germination at supraoptimal temperatures that would be lethal for the developing seedlings. During seed imbibition at supraoptimal temperature, the FUS3 promoter is reactivated and induces de novo synthesis of FUS3 mRNA, followed by FUS3 protein accumulation. Genetic analysis shows that FUS3 contributes to the delay of seed germination at high temperature. Unlike WT, seeds overexpressing FUS3 (ML1:FUS3-GFP) during imbibition are hypersensitive to high temperature and do not germinate, however, they can fully germinate after recovery at control temperature reaching 90% seedling survival. ML1:FUS3-GFP hypersensitivity to high temperature can be partly recovered in the presence of fluridone, an inhibitor of ABA biosynthesis, suggesting this hypersensitivity is due in part to higher ABA level in this mutant. Transcriptomic analysis shows that WT seeds imbibed at supraoptimal temperature activate seed-specific genes and ABA biosynthetic and signaling genes, while inhibiting genes that promote germination and growth, such as GA biosynthetic and signaling genes. In this study, we have uncovered a novel function for the master regulator of seed maturation, FUS3, in delaying germination at supraoptimal temperature. Physiologically, this is

  8. The role of the Arabidopsis FUSCA3 transcription factor during inhibition of seed germination at high temperature

    Directory of Open Access Journals (Sweden)

    Chiu Rex S

    2012-01-01

    Full Text Available Abstract Background Imbibed seeds integrate environmental and endogenous signals to break dormancy and initiate growth under optimal conditions. Seed maturation plays an important role in determining the survival of germinating seeds, for example one of the roles of dormancy is to stagger germination to prevent mass growth under suboptimal conditions. The B3-domain transcription factor FUSCA3 (FUS3 is a master regulator of seed development and an important node in hormonal interaction networks in Arabidopsis thaliana. Its function has been mainly characterized during embryonic development, where FUS3 is highly expressed to promote seed maturation and dormancy by regulating ABA/GA levels. Results In this study, we present evidence for a role of FUS3 in delaying seed germination at supraoptimal temperatures that would be lethal for the developing seedlings. During seed imbibition at supraoptimal temperature, the FUS3 promoter is reactivated and induces de novo synthesis of FUS3 mRNA, followed by FUS3 protein accumulation. Genetic analysis shows that FUS3 contributes to the delay of seed germination at high temperature. Unlike WT, seeds overexpressing FUS3 (ML1:FUS3-GFP during imbibition are hypersensitive to high temperature and do not germinate, however, they can fully germinate after recovery at control temperature reaching 90% seedling survival. ML1:FUS3-GFP hypersensitivity to high temperature can be partly recovered in the presence of fluridone, an inhibitor of ABA biosynthesis, suggesting this hypersensitivity is due in part to higher ABA level in this mutant. Transcriptomic analysis shows that WT seeds imbibed at supraoptimal temperature activate seed-specific genes and ABA biosynthetic and signaling genes, while inhibiting genes that promote germination and growth, such as GA biosynthetic and signaling genes. Conclusion In this study, we have uncovered a novel function for the master regulator of seed maturation, FUS3, in delaying

  9. Cellulolytic (cel) genes of Clostridium thermocellum F7 and the proteins encoded by them

    International Nuclear Information System (INIS)

    Piruzyan, E.S.; Mogutov, M.A.; Velikodvorskaya, G.A.; Pushkarskaya, T.A.

    1988-01-01

    This study is concerned with genes cell, ce12, and ce13 encoding the endoglucanase of the cellulolytic complex of the anaerobic thermophilic Clostridium thermocellum F7 bacteria, these genes having been closed by us earlier. The authors present the characteristics of proteins synthesized by the cel genes in the minicell system of the strain Escherichia coli K-12 X925. The molecular weights of the proteins encoded by genes cell, ce12, and ce13 are 30,000, 45,000, and 50,000 dalton, respectively. The study of the homology of the cloned section of the C. thermocellum DNA containing the endoglucanase genes, using Southern's blot-hybridization method, did not reveal their physical linkage in the genome. The authors detected a plasmid with a size of about 30 kb in the cells of the C. thermocellum F7 strain investigated

  10. Ball-Sport Endurance and Sprint Test (BEAST90): validity and reliability of a 90-minute soccer performance test.

    Science.gov (United States)

    Williams, Jeremy D; Abt, Grant; Kilding, Andrew E

    2010-12-01

    The aim of this study was to determine the validity and reliability of a 90-minute soccer performance test: Ball-sport Endurance and Sprint Test (BEAST90). Fifteen healthy male amateur soccer players participated and attended 5 testing sessions over a 10-day period to perform physiologic and soccer-specific assessments. This included familiarization sessions and 2 full trials of the BEAST90, separated by 7 days. The total 90-minute distance, mean percent peak heart rate (HRpeak), and estimated percent peak oxygen uptake of the BEAST90 were 8,097 ± 458 m, 85 ± 5% and 82 ± 14%, respectively. Measures obtained from trial 1 and trial 2 were not significantly different (p > 0.05). Reliability of measures over 90 minutes ranged from 0.9-25.5% (% typical error). The BEAST90 protocol replicated soccer match play in terms of time, movement patterns, physical demands (volume and intensity), distances, and mean and HRpeak values, as well as having an aerobic load similar to that observed during a soccer match. Reproducibility of key physical measures during the BEAST90 were mostly high, suggesting good reliability. The BEAST90 could be used in studies that wish to determine the effects of training or nutritional interventions on prolonged intermittent physical performance.

  11. Transfer factor of "9"0Sr and "1"3"7Cs to lettuce and winter wheat at different growth stage applications

    International Nuclear Information System (INIS)

    Al Attar, Lina; Al-Oudat, Mohammad; Safia, Bassam; Ghani, Basem Abdul

    2015-01-01

    The effect of clay soil contamination time on the transfer factors (F_vs) of "1"3"7Cs and "9"0Sr was investigated in four different growth stages of winter wheat and lettuce crops. The experiment was performed in an open field using lysimeters. The F_vs were the ratio of the activity concentrations of the radionuclides in crops to those in soil, both as dry weight (Bq kg"−"1). Significant difference of log-F_vs was evaluated using one-way Analysis of Variance (ANOVA). Basically, F_vs of "9"0Sr were higher than those of "1"3"7Cs, despite of the application stage or crop' variety. Higher F_vs for both radionuclides were observed for lettuce in comparison to winter wheat. F_vs of "9"0Sr showed comparable trends for both crops with enhanced F_vs obtained when contamination occurred in early stages, i.e. 1.20 for lettuce and 0.88 and 0.02 for winter wheat, straw and grains, respectively. Despite the fluctuation noted in the pattern of F_vs for "1"3"7Cs, soil contaminated at the second stage gave the highest F_vs for lettuce and grains, with geometric means of 0.21 and 0.01, respectively. However, wheat-straw showed remarkable increase in F_v for the latest contamination (ripening stage), about 0.06. It could be concluded that soil contamination at early growth stages would represent high radiological risk for the scenarios studied with an exception to "1"3"7Cs in winter wheat-straw which reflected greater hazard at the latest application. - Highlights: • Higher TFs for both radionuclides were observed for leafy plant in comparison to cereals. • Despite the growth stages & plants' variety, TFs of "9"0Sr were always higher than those of "1"3"7Cs. • TFs of "9"0Sr showed comparable trends in both crops and were higher at earlier growth stages. • Fluctuation noted in TFs for "1"3"7Cs in lettuce with higher TFs at second contamination-stage. • High TFs for "1"3"7Cs when contamination occurred at the latest growth stage of wheat vegetative.

  12. Manejo nutricional de la enfermedad celíaca Nutritional management of the celiac disease

    Directory of Open Access Journals (Sweden)

    Daris I. González Hernández

    2006-06-01

    Full Text Available La enfermedad celíaca es una causa importante de malaabsorción en la niñez. Se caracteriza por una intolerancia total y permanente al gluten que contiene la fracción proteica del trigo y otros cereales. La patogenia de la enfermedad es multifactorial y tiene un cuadro clínico variado, en el cual predominan los síntomas de malaabsorción y la desnutrición. La dieta exenta de gluten es la parte más importante de la terapéutica, además de hacer frente a las deficiencias nutricionales. Se hace una revisión del manejo dietético y la educación nutricional del paciente y sus familiares, con énfasis en los alimentos que pueden contener gluten de forma enmascarada.

  13. Analyses of surface motions caused by the magnitude 9.0 2004 Sumatra earthquake

    DEFF Research Database (Denmark)

    Khan, Shfaqat Abbas; Gudmundsson, Ó.

    The Sumatra, Indonesia, earthquake on December 26th was one of the most devastating earthquakes in history. With a magnitude of Mw = 9.0 it is the forth largest earthquake recorded since 1900. It occurred about one hundred kilometers off the west coast of northern Sumatra, where the relatively thin...... of years. The result was a devastating tsunami hitting coastlines across the Indian Ocean killing more than 225,000 people in Sri Lanka, India, Indonesia, Thailand and Malaysia. An earthquake of this magnitude is expected to involve a displacement on the fault on the order of 10 meters. But, what...... was the actual amplitude of the surface motions that triggered the tsunami? This can be constrained using the amplitudes of elastic waves radiated from the earthquake, or by direct measurements of deformation. Here we present estimates of the deformation based on continuous Global Positioning System (GPS...

  14. The solvent extraction of carrier-free 90Y from 90Sr with crown ethers

    International Nuclear Information System (INIS)

    Chuang, J.T.; Lo, J.G.

    1995-01-01

    A simple solvent extraction method has been developed for the separation of 90 Y from 90 Sr. Crown ether dissolved in chloroform was used as a selective reagent and organic picrate anion was chosen as a counter ion. The effect of various factors on the extraction equilibrium constant of strontium log K ex = 9.15 was obtained from the study of the distribution coefficient versus to crown ether concentration. The separation method was simple, resulted high purity (>99.9%) and quantitative yield, and took less than half an hour. (author) 27 refs.; 4 figs.; 4 tabs

  15. Molecular and biochemical analyses of the GH44 module of CbMan5B/Cel44A, a bifunctional enzyme from the hyperthermophilic bacterium Caldicellulosiruptor bescii.

    Science.gov (United States)

    Ye, Libin; Su, Xiaoyun; Schmitz, George E; Moon, Young Hwan; Zhang, Jing; Mackie, Roderick I; Cann, Isaac K O

    2012-10-01

    A large polypeptide encoded in the genome of the thermophilic bacterium Caldicellulosiruptor bescii was determined to consist of two glycoside hydrolase (GH) modules separated by two carbohydrate-binding modules (CBMs). Based on the detection of mannanase and endoglucanase activities in the N-terminal GH5 and the C-terminal GH44 module, respectively, the protein was designated CbMan5B/Cel44A. A GH5 module with >99% identity from the same organism was characterized previously (X. Su, R. I. Mackie, and I. K. Cann, Appl. Environ. Microbiol. 78:2230-2240, 2012); therefore, attention was focused on CbMan5A/Cel44A-TM2 (or TM2), which harbors the GH44 module and the two CBMs. On cellulosic substrates, TM2 had an optimal temperature and pH of 85°C and 5.0, respectively. Although the amino acid sequence of the GH44 module of TM2 was similar to those of other GH44 modules that hydrolyzed cello-oligosaccharides, cellulose, lichenan, and xyloglucan, it was unique that TM2 also displayed modest activity on mannose-configured substrates and xylan. The TM2 protein also degraded Avicel with higher specific activity than activities reported for its homologs. The GH44 catalytic module is composed of a TIM-like domain and a β-sandwich domain, which consists of one β-sheet at the N terminus and nine β-sheets at the C terminus. Deletion of one or more β-sheets from the β-sandwich domain resulted in insoluble proteins, suggesting that the β-sandwich domain is essential for proper folding of the polypeptide. Combining TM2 with three other endoglucanases from C. bescii led to modest synergistic activities during degradation of cellulose, and based on our results, we propose a model for cellulose hydrolysis and utilization by C. bescii.

  16. 47 CFR 90.1211 - Regional plan.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Regional plan. 90.1211 Section 90.1211... § 90.1211 Regional plan. (a) To facilitate the shared use of the 4.9 GHz band, each region may submit a plan on guidelines to be used for sharing the spectrum within the region. Any such plan must be...

  17. Investigation of an "alternate water supply system" in enzymatic hydrolysis in the processive endocellulase Cel7A from Rasamsonia emersonii by molecular dynamics simulation.

    Science.gov (United States)

    Sun, Xun; Qian, Meng-Dan; Guan, Shan-Shan; Shan, Ya-Ming; Dong, Ying; Zhang, Hao; Wang, Song; Han, Wei-Wei

    2017-02-01

    Cel7A from Rasamsonia emersonii is one of the processive endocellulases classified under family 7 glycoside hydrolase. Molecular dynamics simulations were carried out to obtain the optimized sliding and hydrolyzing conformations, in which the reducing ends of sugar chains are located on different sites. Hydrogen bonds are investigated to clarify the interactions between protein and substrate in either conformation. Nine hydrogen bonding interactions are identified in the sliding conformation, and six similar interactions are also found correspondingly in the hydrolyzing conformation. In addition, four strong hydrophobic interactions are also determined. The domain cross-correlation map analysis shows movement correlation of protein including autocorrelation between residues. The root mean square fluctuations analysis represents the various flexibilities of different fragment in the two conformations. Comparing the two conformations reveals the water-supply mechanism of selective hydrolysis of cellulose in Cel7A. The mechanism can be described as follow. When the reducing end of substrate slides from the unhydrolyzing site (sliding conformation) to the hydrolyzing site (hydrolyzing conformation), His225 is pushed down and rotated, the rotation leads to the movement of Glu209 with the interstrand hydrogen bonding in β-sheet. It further makes Asp211 close to the hydrolysis center and provides a water molecule bounding on its carboxyl in the previous unhydrolyzing site. After the hydrolysis takes place and the product is excluded from the enzyme, the Asp211 comes back to its initial position. In summary, Asp211 acts as an elevator to transport outer water molecules into the hydrolysis site for every other glycosidic bond. © 2016 Wiley Periodicals, Inc.

  18. Production of Strontium-90 Thermal Power Sources; Fabrication de sources d'energie thermique au strontium-90; Proizvodstvo istochnikov ''teplovoj ehnergii iz Sr''9''0; Preparacion de fuentes de energia termica con estroncio-90

    Energy Technology Data Exchange (ETDEWEB)

    Cochran, J. S.; Bloom, J. L.; Schneider, A. [Martin Company, Nuclear Division, Baltimore 3, MD (United States)

    1963-11-15

    One of the most attractive fields for utilization of large quantities of waste fission products is the field of direct-conversion power supplies for remote locations. Strontium-90 is being given the greatest exploitation because of its availability, nuclear properties, and the relative ease with which it can be fabricated into compact heat sources. Strontium-90 fuelled generators are being used to power automatic weather stations and navigational aids, and consideration is being given to the use of strontium-90 as a power source for space vehicles. Evaluation of several potentially useful strontium compounds led to the selection of the titanate as exhibiting overall properties most desirable for this purpose. Strontium-90, separated from crude fission product streams and purified to the requisite degree by the USAEC's Hanford Works, is shipped in the form of the carbonate to a hot cell facility operated by the Martin Company, where it is converted to titanate pellets. This process is an adaption to remote operation of conventional chemical and ceramic techniques. The pellets are encapsulated in Hastelloy C containers for use in thermoelectric power supplies. Unusual operational problems are encountered because the large quantities of strontium-90 handled (potentially millions of curies per year) represent formidable radiation and contamination hazards. Details of the facility, equipment, process, and safety criteria are given. The operational experience gained during the recent processing of the first 250 000 curies of strontium-90 into fuel for a SNAP-7 generator is described. Encapsulation, calorimetry, decontamination, and waste disposal procedures are also outlined. (author) [French] L'une des utilisations les plus interessantes des produits de fission en grande quantite consiste a les employer comme sources d'energie par combustion directe pour des installations geographiquement isolees. C'est le strontium-90 qui est l e plus utilise parce qu'on en dispose en

  19. Microbial Production of Malic Acid from Biofuel-Related Coproducts and Biomass

    Directory of Open Access Journals (Sweden)

    Thomas P. West

    2017-04-01

    Full Text Available The dicarboxylic acid malic acid synthesized as part of the tricarboxylic acid cycle can be produced in excess by certain microorganisms. Although malic acid is produced industrially to a lesser extent than citric acid, malic acid has industrial applications in foods and pharmaceuticals as an acidulant among other uses. Only recently has the production of this organic acid from coproducts of industrial bioprocessing been investigated. It has been shown that malic acid can be synthesized by microbes from coproducts generated during biofuel production. More specifically, malic acid has been shown to be synthesized by species of the fungus Aspergillus on thin stillage, a coproduct from corn-based ethanol production, and on crude glycerol, a coproduct from biodiesel production. In addition, the fungus Ustilago trichophora has also been shown to produce malic acid from crude glycerol. With respect to bacteria, a strain of the thermophilic actinobacterium Thermobifida fusca has been shown to produce malic acid from cellulose and treated lignocellulosic biomass. An alternate method of producing malic acid is to use agricultural biomass converted to syngas or biooil as a substrate for fungal bioconversion. Production of poly(β-l-malic acid by strains of Aureobasidium pullulans from agricultural biomass has been reported where the polymalic acid is subsequently hydrolyzed to malic acid. This review examines applications of malic acid, metabolic pathways that synthesize malic acid and microbial malic acid production from biofuel-related coproducts, lignocellulosic biomass and poly(β-l-malic acid.

  20. A Novel GH7 Endo-β-1,4-Glucanase from Neosartorya fischeri P1 with Good Thermostability, Broad Substrate Specificity and Potential Application in the Brewing Industry.

    Directory of Open Access Journals (Sweden)

    Yun Liu

    Full Text Available An endo-β-1,4-glucanase gene, cel7A, was cloned from the thermophilic cellulase-producing fungus Neosartorya fischeri P1 and expressed in Pichia pastoris. The 1,410-bp full-length gene encodes a polypeptide of 469 amino acids consisting of a putative signal peptide at residues 1-20, a catalytic domain of glycoside hydrolase family 7 (GH7, a short Thr/Ser-rich linker and a family 1 carbohydrate-binding module (CBM 1. The purified recombinant Cel7A had pH and temperature optima of pH 5.0 and 60°C, respectively, and showed broad pH adaptability (pH 3.0-6.0 and excellent stability at pH3.0-8.0 and 60°C. Belonging to the group of nonspecific endoglucanases, Cel7A exhibited the highest activity on barley β-glucan (2020 ± 9 U mg-1, moderate on lichenan and CMC-Na, and weak on laminarin, locust bean galactomannan, Avicel, and filter paper. Under simulated mashing conditions, addition of Cel7A (99 μg reduced the mash viscosity by 9.1% and filtration time by 24.6%. These favorable enzymatic properties make Cel7A as a good candidate for applications in the brewing industry.

  1. Quantification of Nε-(2-Furoylmethyl)-L-lysine (furosine), Nε-(Carboxymethyl)-L-lysine (CML), Nε-(Carboxyethyl)-L-lysine (CEL) and total lysine through stable isotope dilution assay and tandem mass spectrometry.

    Science.gov (United States)

    Troise, Antonio Dario; Fiore, Alberto; Wiltafsky, Markus; Fogliano, Vincenzo

    2015-12-01

    The control of Maillard reaction (MR) is a key point to ensure processed foods quality. Due to the presence of a primary amino group on its side chain, lysine is particularly prone to chemical modifications with the formation of Amadori products (AP), Nε-(Carboxymethyl)-L-lysine (CML), Nε-(Carboxyethyl)-L-lysine (CEL). A new analytical strategy was proposed which allowed to simultaneously quantify lysine, CML, CEL and the Nε-(2-Furoylmethyl)-L-lysine (furosine), the indirect marker of AP. The procedure is based on stable isotope dilution assay followed by liquid chromatography tandem mass spectrometry. It showed high sensitivity and good reproducibility and repeatability in different foods. The limit of detection and the RSD% were lower than 5 ppb and below 8%, respectively. Results obtained with the new procedure not only improved the knowledge about the reliability of thermal treatment markers, but also defined new insights in the relationship between Maillard reaction products and their precursors. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Development of techniques for rapid analysis of 90Sr breakthrough and 90Y activity from a 90Sr-90Y generator

    International Nuclear Information System (INIS)

    Chism, S.E.; Goodwin, D.A.; Meares, C.F.

    1986-01-01

    Recently there has been interest in labelling monoclonal antibodies with therapeutic amounts of a pure beta emitting isotope. 90 Y offers many desirable features and may be conveniently and economically obtained from a 90 Sr- 90 Y generator. A potential hazard with this system is breakthrough of the parent 90 Sr which has a half life of 28 years and is an extremely toxic bone seeking isotope. Therefore it is essential that the daughter be completely separated from its parent. We have devised two simple techniques which allow us to monitor rapidly the amount of 90 Sr breakthrough and quantitate the 90 Y activity over a 5 log range. (author)

  3. Timing of births in sympatric brown howler monkeys (Alouatta fusca clamitans) and northern muriquis (Brachyteles arachnoides hypoxanthus).

    Science.gov (United States)

    Strier, K B; Mendes, S L; Santos, R R

    2001-10-01

    We monitored the birth patterns of sympatric brown howler monkeys (Alouatta fusca clamitans) and northern muriquis (Brachyteles arachnoides hypoxanthus) during a 4-yr period from October 1996 to August 2000 at the Estação Biológica de Caratinga, Minas Gerais, Brazil. Brown howler monkey births (n = 34) occurred throughout the year, and birth frequencies did not differ between rainy and dry season months. The aseasonal birth patterns of the howler monkeys differed significantly from the dry season concentration and dry month peak in muriqui births (n = 23). We found no effects of infant sex or the number of females on interbirth intervals (IBIs) in our 10 howler monkey study troops. IBIs of brown howler monkeys averaged 21.2 +/- 2.5 mo (n = 8, median = 21.0 mo), and were significantly shorter following dry season births than rainy season births. Their IBIs and yearling survivorship (74%) were similar to those reported for other species of howler monkeys, but yearling survivorship was much lower than that of muriquis (94%), whose IBIs were more than 12 mo longer than those of the howler monkeys. Our study extends comparative knowledge of birth patterns in Alouatta to a poorly known species, and provides insights into the different ways in which diet and life history may affect the timing of births in large-bodied platyrrhines under the same seasonal ecological conditions. Copyright 2001 Wiley-Liss, Inc.

  4. Fondurile cinegetice ale Universității ”Ștefan cel Mare” Suceava – Facultatea de Silvicultură: o retrospectivă a activității [Hunting Funds of Ștefan cel Mare University of Suceava - Faculty of Forestry: a retrospective of work

    Directory of Open Access Journals (Sweden)

    Dănilă G

    2016-12-01

    Full Text Available Ștefan cel Mare University’s Hunting Funds, through the Faculty of Forestry, consists of three hunting territories (HT: HT no. 55 Mitoc, HT no. 69 Râșca, HT no. 56 Salcea. The first two have a high hunting potential for major hunting sedentary game species (wild boar, roe buck, roe deer, hare, wolf, bear. In over 15 years of hunting management, USV has overcome the difficulties imposed by different legislations. Also, USV, along with the University of Brașov, managed to amend Law 407/2006 and to return to free hunting use of the public lands. The teaching and research activities in the hunting territories have resulted in over 15 diploma projects, over 20 scientific articles published in specialized reviews, of which 7 ISI, over 10 scientific papers of which two were the students’ work, two research contracts and a book. The management coherence driven by the specialized staff is reflected in the higher number of top quality trophies, which started to be harvested after about 10 years of management.

  5. Caracterización bioinformática de unidades de transcripción no anotadas relacionadas con la enfermedad celíaca

    OpenAIRE

    Fernández Portela, Silvia

    2016-01-01

    La celiaquía es una enfermedad compleja y crónica que se debe a una reacción de intolerancia a la ingesta del gluten. Sus vías moleculares son poco conocidas aunque han sido muy estudiadas debido a que es multigénica. Se presenta la secuenciación de ARN como método de identificación de nuevos transcritos por todo el genoma para la compresión de la enfermedad celíaca. Pudiendo extrapolarse a cualquier otra investigación. Para ello predeciremos transcritos del RNAseq, los identificamos y caract...

  6. Exploitation of 3D face-centered cubic mesoporous silica as a carrier for a poorly water soluble drug: influence of pore size on release rate.

    Science.gov (United States)

    Zhu, Wenquan; Wan, Long; Zhang, Chen; Gao, Yikun; Zheng, Xin; Jiang, Tongying; Wang, Siling

    2014-01-01

    The purposes of the present work were to explore the potential application of 3D face-centered cubic mesoporous silica (FMS) with pore size of 16.0nm as a delivery system for poorly soluble drugs and investigate the effect of pore size on the dissolution rate. FMS with different pore sizes (16.0, 6.9 and 3.7nm) was successfully synthesized by using Pluronic block co-polymer F127 as a template and adjusting the reaction temperatures. Celecoxib (CEL), which is a BCS class II drug, was used as a model drug and loaded into FMS with different pore sizes by the solvent deposition method at a drug-silica ratio of 1:4. Characterization using scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transformation infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), nitrogen adsorption, X-ray diffraction (XRD), and differential scanning calorimetry (DSC) was used to systematically investigate the drug loading process. The results obtained showed that CEL was in a non-crystalline state after incorporation of CEL into the pores of FMS-15 with pore size of 16.0nm. In vitro dissolution was carried out to demonstrate the effects of FMS with different pore sizes on the release of CEL. The results obtained indicated that the dissolution rate of CEL from FMS-15 was significantly enhanced compared with pure CEL. This could be explained by supposing that CEL encountered less diffusion resistance and its crystallinity decreased due to the large pore size of 16.0nm and the nanopore channels of FMS-15. Moreover, drug loading and pore size both play an important role in enhancing the dissolution properties for the poorly water-soluble drugs. As the pore size between 3.7 and 16.0nm increased, the dissolution rate of CEL from FMS gradually increased. © 2013.

  7. Estudo anatômico dos gânglios celíaco, celiacomesentérico e mesentérico cranial e de suas conexões no gato doméstico (Felix domestica, Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Antonio Augusto Coppi Maciel Ribeiro

    2000-01-01

    Full Text Available O gânglio celíaco é um dos principais responsáveis pela inervação do estômago, intestinos, fígado, pâncreas e ainda contribui para a inervação do baço, sendo desta forma essencial ao controle da motilidade gastrointestinal. O conhecimento do suprimento nervoso endereçado a estes órgãos é fundamental na clínica médica e cirúrgica no tocante às atonias digestivas, gastroenterites hemorrágicas, torções gástricas e invaginações intestinais. Neste trabalho, estudou-se a anatomia macro e microscópica dos gânglios celíaco, celiacomesentérico e mesentérico cranial. Foram utilizados trinta gatos domésticos, adultos, 10 machos e 20 fêmeas. A aorta torácica desses gatos foi injetada com solução de Neoprene látex 650 corada, sendo os animais congelados por no mínimo 48 horas. A fixação foi feita posteriormente com solução aquosa de formol a 10%. Nos estudos microscópicos, utilizaram-se as seguintes colorações: hematoxilina-eosina, tricrômico de Masson, reticulina e hematoxilina ácida fosfotúngstica. O gânglio celíaco ocorreu 7 vezes, sendo 4 direitos e 3 esquerdos, predominando a forma elíptica (23,3% e a situação periarterial. Os gânglios celiacomesentéricos foram contados em número de 24, sendo 11 independentes; 2 apresentando as porções direita e esquerda e 11 gânglios celiacomesentéricos direitos com uma porção mesentérica cranial esquerda, tendo formato semilunar assimétrico. Esses achados sugerem o predomínio da fusão do gânglio celíaco ao mesentérico cranial, constituindo assim o gânglio celiacomesentérico. Este é formado por neurônios imersos em abundante matriz conjuntiva fibrosa, envoltos por uma cápsula contendo fibras elásticas, colágenas e reticulares, apresentando uma continuidade nos pontos de fusão ganglionar.

  8. Biomass production of the marine microalga; chroomonas sp. in function of the pH, luminous intensity and salinity

    International Nuclear Information System (INIS)

    Bermudez, Jose Luis; Lodeiros, Cesar; Morales, Ever

    2002-01-01

    We report the characterization of a marine microalga of the genus Chroomonas, isolated from a salt lagoon located to the north of Maracaibo, Zulia State, Venezuela. We evaluated the growth and the pigment production in discontinuous culture at different salinities (5, 10, 35, 50, 70 y 100 ppm), light intensities (39,78,117 and 156 μmol quanta.m 2 . s 1 and pH (5.0, 5.5, 6.0, 7.0, 8.0 and 9.0). The highest cellular density, 117.99±2.62x10 6 fg.cel l , was reached at 35 ppm, 156 μmol quanta.m 2 . s 1 of light intensity and a ph between 6.0 and 8.0. The cellular content of total chlorophyll and carotenoids increased with the salinity up to 100 ppm, with amounts of 246.55 ± 61.8 y 69.79±18.19 fg.cel l , respectively. The cellular productivity 4.31x10 9 cel 1 d 1 was obtained when the microalga, was grown in semi-continuous culture, at a 2.01 volume and at a daily renewal rate of 30 % (v/v). The total amount of chlorophyll and carotenoids was 1.4 and 0.48 mg.l d , respectively. These results indicate that this planktonic microalga could be used as daily live food for larvae in aquaculture and for the production of micro algal biomass and/ or pigments

  9. Exploitation of 3D face-centered cubic mesoporous silica as a carrier for a poorly water soluble drug: Influence of pore size on release rate

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Wenquan; Wan, Long; Zhang, Chen; Gao, Yikun; Zheng, Xin; Jiang, Tongying; Wang, Siling, E-mail: silingwang@syphu.edu.cn

    2014-01-01

    The purposes of the present work were to explore the potential application of 3D face-centered cubic mesoporous silica (FMS) with pore size of 16.0 nm as a delivery system for poorly soluble drugs and investigate the effect of pore size on the dissolution rate. FMS with different pore sizes (16.0, 6.9 and 3.7 nm) was successfully synthesized by using Pluronic block co-polymer F127 as a template and adjusting the reaction temperatures. Celecoxib (CEL), which is a BCS class II drug, was used as a model drug and loaded into FMS with different pore sizes by the solvent deposition method at a drug–silica ratio of 1:4. Characterization using scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transformation infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), nitrogen adsorption, X-ray diffraction (XRD), and differential scanning calorimetry (DSC) was used to systematically investigate the drug loading process. The results obtained showed that CEL was in a non-crystalline state after incorporation of CEL into the pores of FMS-15 with pore size of 16.0 nm. In vitro dissolution was carried out to demonstrate the effects of FMS with different pore sizes on the release of CEL. The results obtained indicated that the dissolution rate of CEL from FMS-15 was significantly enhanced compared with pure CEL. This could be explained by supposing that CEL encountered less diffusion resistance and its crystallinity decreased due to the large pore size of 16.0 nm and the nanopore channels of FMS-15. Moreover, drug loading and pore size both play an important role in enhancing the dissolution properties for the poorly water-soluble drugs. As the pore size between 3.7 and 16.0 nm increased, the dissolution rate of CEL from FMS gradually increased. - Highlights: • Exploitation of 3D cubic mesoporous silica (16 nm) as a carrier was completed. • The release rate of CEL increased on increasing the pore size of carriers. • The crystallinity

  10. Efecto de la incorporación de fibras dietéticas en la calidad de panes para celíacos

    OpenAIRE

    Díaz Malmierca, Álvaro

    2013-01-01

    La investigación en la elaboración de panes sin gluten está muy avanzada, siendo el déficit en micronutrientes de estos productos uno de los problemas sobre el que se está centrando la investigación. Uno de los nutrientes más deficitarios, y que más problemas causa sobre la salud de los pacientes celíacos es la fibra dietética. En este estudio se ha investigado el efecto que produce la adición de un 10% de diferentes fibras sobre las características de los panes, la influencia ...

  11. Extractive {sup 90}Y generator

    Energy Technology Data Exchange (ETDEWEB)

    Kodina, G.E. [State Scientific Center, Moscow (Russian Federation). Institute of Giophysics; Korpusov, G.V.; Filyanin, A.T. [Russian Academy of Sciences, Moscow (Russian Federation). Institute of Physical Chemistry

    1997-10-01

    The generator for {sup 90}Y production is made up of two units - an extractive unit and a unit for deep purification. Contrary to the well-known methods of {sup 90}Y separation two mineral acids are used in the developed technology. The solutions of nitric acid are used for preliminary separation of {sup 90}Y and the solutions of hydrochloric acid are used for the deep purification of {sup 90}Y from {sup 90}Sr and stripping. The optimum conditions for carrying out this technology were found. The following results are reported: the carry-over of the stationary phase is excluded in this generator; the separation time is 30-60 minutes; the yield of the final product is not less than 95%; impurity of {sup 90}Sr is not more than 10{sup -9-}-{sup -10}%; the content of the chemical (nonactive) impurities is not more than the allowed one for radiopharmaceuticals 4 refs., 4 tabs., 5 figs.

  12. Tohoku's earthquake of Friday March 11, 2011 (5:46 UT), magnitude 9.0, off Honshu island (Japan)

    International Nuclear Information System (INIS)

    2011-01-01

    On Friday March 11, 2011, at 5:46 UT (2:46 PM local time), a magnitude 9.0 earthquake took place at 80 km east of Honshu island (Japan). The earthquake generated a tsunami which led to the loss of the cooling systems of the Fukushima Dai-ichi and Fukushima Daini power plants. This paper describes the seismo-tectonic and historical seismic context of the Japan archipelago and the first analyses of the Tohoku earthquake impact: magnitudes of first shock and of aftershocks, impact on nuclear facilities (maximum acceleration values detected with respect to design basis values, subsidence of coastal areas and submersion of power plant platforms). (J.S.)

  13. Epidemiological impact of achieving UNAIDS 90-90-90 targets for HIV care in India: a modelling study.

    Science.gov (United States)

    Maddali, Manoj V; Gupta, Amita; Shah, Maunank

    2016-07-07

    Recent UNAIDS '90-90-90' targets propose that to end the HIV epidemic by 2030, 90% of persons living with HIV (PLWH) worldwide should know their diagnosis, 90% of diagnosed PLWH should be on antiretroviral therapy (ART) and 90% of PLWH on ART should be virally suppressed by 2020. We sought to quantify the epidemiological impact of achieving these targets in India. We constructed a dynamic-transmission model of the Indian HIV epidemic to project HIV infections and AIDS-related deaths that would occur in India over 15 years. We considered several scenarios: continuation of current care engagement (with early ART initiation), achieving 90-90-90 targets on time and delaying achievement by 5 or 10 years. In the base case, assuming continuation of current care engagement, we project 794 000 (95% uncertainty range (UR) 571 000-1 104 000) HIV infections and 689 000 (95% UR 468 000-976 000) AIDS-related deaths in India over 15 years. In this scenario, nearly half of PLWH diagnosed would fail to achieve viral suppression by 2030. With achievement of 90-90-90 targets, India could avert 392 000 (95% UR 248 000-559 000) transmissions (48% reduction) and 414 000 (95% UR 260 000-598 000) AIDS-related deaths (59% reduction) compared to the base-case scenario. Furthermore, fewer than 20 000 (95% UR 12 000-30 000) HIV infections would occur in 2030. Delaying achievement of targets resulted in a similar reduction in HIV incidence by 2030 but at the cost of excess overall infections and mortality. India can halve the epidemiological burden of HIV over 15 years with achievement of the UNAIDS 90-90-90 targets. Reaching the targets on time will require comprehensive healthcare strengthening, especially in early diagnosis and treatment, expanded access to second-line and third-line ART and long-term retention in care. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  14. Doença celíaca: a evolução dos conhecimentos desde sua centenária descrição original até os dias atuais Celiac disease: evolution in knowledge since its original centennial description up to the present days

    Directory of Open Access Journals (Sweden)

    Vera Lucia SDEPANIAN

    1999-12-01

    Full Text Available Nos últimos anos, alguns aspectos da doença celíaca têm sido discutidos na literatura, especialmente relacionados à predisposição genética, patogênese, formas de apresentação clínica e critérios diagnósticos. Inúmeros estudos demonstraram anormalidades imunológicas características da doença como a presença de anticorpos circulantes e de linfócitos com receptores gama/delta presentes em grande número a nível intraepitelial da mucosa intestinal. Outras formas de apresentação clínica, além da forma clássica, têm merecido destaque como baixa estatura, anemia resistente à ferroterapia oral, hipoplasia do esmalte dentário, constipação intestinal, manifestações neurológicas e osteoporose, dentre outras. A forma assintomática foi reconhecida especialmente nas duas últimas décadas após o desenvolvimento de marcadores sorológicos como anticorpo antigliadina, anti-reticulina e antiendomísio. Até o presente momento, a biopsia de intestino delgado continua sendo imprescindível para o diagnóstico da doença celíaca. No Brasil, fatos marcantes ocorreram nos últimos anos, como a promulgação da Lei Federal que dispõe sobre a obrigatoriedade dos rótulos dos produtos industrializados informarem sobre a presença de glúten. Houve, também, aumento do número de portadores de doença celíaca cadastrados na Associação dos Celíacos do Brasil.In the recent past, some celiac disease features have been discussed in literature specially related to genetic susceptibility, pathogenesis, clinical presentation and diagnostic criteria. Immunological abnormalities characteristic of celiac disease, such as circulating antibodies and increased numbers of intra-epithelial lymphocytes containing a high percentage of gamma-delta T cells have been demonstrated. Other pictures of clinical presentation besides the classical one deserve attention namely short stature, iron-resistant anaemia, enamel hypoplasia, constipation

  15. Separation of 90Sr from Purex high level waste and development of a 90Sr-90Y generator

    International Nuclear Information System (INIS)

    Ramanujam, A.; Dhami, P.S.; Chitnis, R.R.; Achuthan, P.V.; Kannan, R.; Gopalakrishnan, V.; Balu, K.

    2000-04-01

    90 Y (T 1/2 =64.2 h) finds several applications in nuclear medicine. It is formed from the decay of 90 Sr which has a long half-life of 28.8 years. 90 Sr can be used as a long-lasting source for the production of carrier-free 90 Y. 90 Sr itself is abundantly available in high level waste (HLW) of PUREX origin. The present studies deal with the separation of pure 90 Sr from HLW and the subsequent separation of 90 Y from 90 Sr. Actinides and some of the fission products like lanthanides, zirconium, molybdenum and cesium were first removed from the HLW using methods based on solvent extraction and ion-exchange studied earlier in our laboratory. The resulting waste solution was used as a feed for the present process. The separation of 90 Sr from HLW was based on radiochemical method which involved a repeated scavenging with ferric hydroxide followed by strontium carbonate precipitation. The separation of 90 Y from 90 Sr was achieved by membrane separation technique. A compact generator is developed for this separation using a commercially available polytetrafluoroethylene (PTFE) membrane, impregnated with indigenously synthesised 2-ethylhexyl 2-ethylhexyl phosphonic acid (KSM-17). Generator system overcomes the drawbacks associated with conventional solvent extraction and ion-exchange based generators. The product is in chloride form and is suitable for complexation studies. After gaining an operating experience of ∼3 years in generating carrier-free 90 Y at 2 mCi level for initial studies in radiotherapeutic applications, the process was scaled up for the production of about 12 mCi of 90 Y to be used for animal studies before its application to patients. Radiochemical and chemical purity of the product was critically assayed by radiometry, ICP-AES, etc. The process is amenable for further scaling up. (author)

  16. Desarrollo de métodos cuantitativos de alto poder de detección de proteínas en alimentos : Certificación de alimentos destinados a enfermos celíacos

    OpenAIRE

    Doña, Vanina V.

    2009-01-01

    La enfermedad celíaca (EC) es una enteropatía crónica mediada por mecanismos inmunológicos, que se desencadena en individuos genéticamente susceptibles por la ingestión de un grupo de proteínas (llamadas prolaminas) presentes en trigo, cebada, centeno y avena (aunque se debate sobre la toxicidad de esta última). La activación del sistema inmune genera alteraciones histológicas típicas (atrofia vellositaria, hiperplasia de criptas) y funcionales en la mucosa intestinal, produciendo un síndrome...

  17. Design and characterizations of two novel cellulases through single-gene shuffling of Cel12A (EG3) gene from Trichoderma reseei.

    Science.gov (United States)

    Yenenler, Asli; Sezerman, Osman Ugur

    2016-06-01

    Cellulases have great potential to be widely used for industrial applications. In general, naturally occurring cellulases are not optimized and limited to meet the industrial needs. These limitations lead to demand for novel cellulases with enhanced enzymatic properties. Here, we describe the enzymatic and structural properties of two novel enzymes, EG3_S1 and EG3_S2, obtained through the single-gene shuffling approach of Cel12A(EG3) gene from Trichoderma reseei EG3_S1 and EG3_S2 shuffled enzymes display 59 and 75% identity in protein sequence with respect to native, respectively. Toward 4-MUC, the minimum activity of EG3_S1 was reported as 5.9-fold decrease in native at 35°C, whereas the maximum activity of EG3_S2 was reported as 15.4-fold increase in native activity at 40°C. Also, the diminished enzyme activity of EG3_S1 was reported within range of 0.6- to 0.8-fold of native and within range of 0.5- to 0.7-fold of native toward CMC and Na-CMC, respectively. For EG3_S2 enzyme, the improved enzymatic activities within range of 1.1- to 1.4-fold of native and within range of 1.1- to 1.6-fold of native were reported toward CMC and Na-CMC, respectively. Moreover, we have reported 6.5-fold increase in the kcat/Km ratio of EG3_S2 with respect to native and suggested EG3_S2 enzyme as more efficient catalysis for hydrolysis reactions than its native counterpart. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  18. Van Nuys, California. Limited Surface Observations Climatic Summary ’LISOCS.’ Parts A-F.

    Science.gov (United States)

    1987-08-04

    MONTH: JUL HOURS(LSI: 0600-39CO I WIND SPEE) TN KNOTS DIRECTION I 1-3 4 -6 i-10 121-16 17-2 1 22-??7 28- 33 34-4O NT1 -Ri 7 4-55 OF 56 TOTAL MEAN...NO CElL 1 89.1 9.1 54.6 55.7 S6.9 6.9 57.1 57.2 57.2 𔄁. 1. 1 97.3 57.! 97.3 51,’ 47.3 bE LOUCO 93.9 59.4 63.1 61.4 62.6 62.9 63.2 63.3 63...NO CElL I 77.: 47.4 5z.6 54.9 56.7 57.7 59.5 59.5 59.5 59.5 57.81 51 59.5 "q .5 59.5

  19. Estudio de la enfermedad celíaca mediante el desarrollo de un ensayo inmunocromatográfico para la determinación de anticuerpos antitransglutaminasa

    OpenAIRE

    Galván Cabrera, José Armando

    2012-01-01

    En el presente trabajo se muestran los resultados del desarrollo y la evaluación de un sistema inmunocromatográfico rápido y sencillo para la detección de anticuerpos antitransglutaminasa, que es un importante marcador serológico en el diagnóstico de la enfermedad celíaca (EC). Con este sistema se pueden detectar en una misma prueba tanto anticuerpos de tipo IgA como IgG. Se obtuvieron niveles de sensibilidad, especificidad y concordancia elevados cuando se compararon con la biopsia de yeyuno...

  20. Strontium-90 concentrations in pronghorn antelope bones near a nuclear fuel reprocessing plant

    International Nuclear Information System (INIS)

    Markham, O.D.; Halford, D.K.

    1980-01-01

    Metacarpal bones were collected from pronghorn antelope near a nuclear fuel reprocessing plant and adjacent areas on the Idaho National Engineering Laboratory (INEL) Site in Southeastern Idaho. Control bones were collected from offsite animals at higher elevations. Average concentrations in metacarpals were 9.6+-2.8(SE) pCi/g(ash) within 10 km of the Idaho Chemical Processing Plant (ICPP), 4.0+-0.9pCi/g for animals on the remainder of the INEL Site and 5.5+-1.0pCi/g for control animals. ICPP atmospheric releases of 90 Sr appeared to have caused a significant (P 90 Sr concentrations in antelope bones within 10 km of the ICPP as compared to bones of other INEL antelope. However, the ICPP antelope bone 90 Sr concentrations were not statistically different from that occurring in bones of the control animals from higher elevations. Antelope near the ICPP received approximately double the radiation dose to bone compared to doses received by other INEL antelope as a result of 90 Sr in bone. Strontium-90 in bone from both fallout and ICPP sources resulted in an estimated average radiation dose of 40 mrad/yr to edosteal cells and 20 mrad/yr to active bone marrow. (author)

  1. Heat Shock Protein 90 (HSP90 and Her2 in Adenocarcinomas of the Esophagus

    Directory of Open Access Journals (Sweden)

    Julia Slotta-Huspenina

    2014-06-01

    Full Text Available Her2 overexpression and amplification can be found in a significant subset of esophageal adenocarcinomas. The activity of Her2 has been shown to be modulated by molecular chaperones such as HSP90. We analyzed expression/amplification data for HSP90 and Her2 on 127 primary resected esophageal adenocarcinomas in order to evaluate a possible relationship between these two molecules. HSP90 expression determined by immunohistochemistry was observed in various levels. Thirty nine (39 tumors (30.7% were classified as Her2-positive according to their immunoreactivity and amplification status. There was a significant correlation between HSP90 expression and Her2-status (p = 0.008. This could also be demonstrated by quantitative protein expression analysis with reverse phase protein arrays (r = 0.9; p < 0.001. Her2-status was associated withpT-category (p = 0.041, lymph node metastases (p = 0.049 and tumor differentiation (p = 0.036 with a higher percentage of cases with negative Her2 status in lower tumor stagesA negative Her2-status was also associated with better survival in univariate and multivariate analysis (p = 0.001 and p = 0.014. For HSP90, no associations between clinical and pathological parameters were found. The observed association between HSP90 expression and Her2 suggests a co-regulation of these molecules in at least a subset of esophageal adenocarcinomas. Anti-HSP90 drugs, which recently have been introduced in cancer treatment, may also be an option for these tumors by targeting HSP90 alone or in combination with Her2.

  2. Heat Shock Protein 90 (HSP90) and Her2 in Adenocarcinomas of the Esophagus

    Energy Technology Data Exchange (ETDEWEB)

    Slotta-Huspenina, Julia; Becker, Karl-Friedrich [Institute of Pathology, Technische Universität München, München 81765 (Germany); Feith, Marcus [Department of Surgery, Klinikum Rechts der Isar der Technischen Universität München, München 81622 (Germany); Walch, Axel [Institute of Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Neuherberg 85764 (Germany); Langer, Rupert, E-mail: rupert.langer@pathology.unibe.ch [Institute of Pathology, University of Bern, Bern 3010 (Switzerland)

    2014-06-27

    Her2 overexpression and amplification can be found in a significant subset of esophageal adenocarcinomas. The activity of Her2 has been shown to be modulated by molecular chaperones such as HSP90. We analyzed expression/amplification data for HSP90 and Her2 on 127 primary resected esophageal adenocarcinomas in order to evaluate a possible relationship between these two molecules. HSP90 expression determined by immunohistochemistry was observed in various levels. Thirty nine (39) tumors (30.7%) were classified as Her2-positive according to their immunoreactivity and amplification status. There was a significant correlation between HSP90 expression and Her2-status (p = 0.008). This could also be demonstrated by quantitative protein expression analysis with reverse phase protein arrays (r = 0.9; p < 0.001). Her2-status was associated withpT-category (p = 0.041), lymph node metastases (p = 0.049) and tumor differentiation (p = 0.036) with a higher percentage of cases with negative Her2 status in lower tumor stagesA negative Her2-status was also associated with better survival in univariate and multivariate analysis (p = 0.001 and p = 0.014). For HSP90, no associations between clinical and pathological parameters were found. The observed association between HSP90 expression and Her2 suggests a co-regulation of these molecules in at least a subset of esophageal adenocarcinomas. Anti-HSP90 drugs, which recently have been introduced in cancer treatment, may also be an option for these tumors by targeting HSP90 alone or in combination with Her2.

  3. Status and methodology of publicly available national HIV care continua and 90-90-90 targets: A systematic review.

    Directory of Open Access Journals (Sweden)

    Reuben Granich

    2017-04-01

    ," respectively. The limitations to consider when interpreting the results include significant variation in methods used to determine national continua and the possibility that complete continua were not available through our comprehensive search of the public domain.Relatively few complete national continua of care are available in the public domain, and there is considerable variation in the methods for determining progress towards the 90-90-90 target. Despite bearing the highest HIV burden, national care continua from sub-Saharan Africa were less likely to be in the public domain. A standardized monitoring and evaluation approach could improve the use of scarce resources to achieve 90-90-90 through improved transparency, accountability, and efficiency.

  4. Radiologically assisted percutaneous gastrostomy or enterostomy. A retrospective analysis of 90 procedures

    International Nuclear Information System (INIS)

    Mildenberger, P.; Oberholzer, K.; Kauczor, H.U.; Dueber, C.; Kurz, S.; Schild, H.; Thelen, M.

    1996-01-01

    To analyse retrospectively the indications, technique and results of radiologically induced gastrostomies or enterostomies. Radiologically assisted gastrostomies or enterostomies were induced in 90 patients, mostly with high grade obstructions due to head and neck tumours or oesophageal tumours. 19/90 patients had had previous resection of the oesophagus or gastric operations. A catheter was successfully introduced in all patients. In 11/90 patients this was followed by a two-stage procedure. In 16/90 patients puncture was performed under CT control because of some anatomical peculiarity. Serious complication occurred in 9/90 patients but in only one was surgery necessary. There were no deaths resulting from the procedure but mortality after 30 days was 6.7%. Radiologically assisted gastrostomies or enterostomies are a reliable and effective form of treatment even amongst difficult patients. The results and complications are comparable to those from endoscopic procedures. (orig.) [de

  5. Preparation of 90Sr-90Y generator

    International Nuclear Information System (INIS)

    Jin Xiaohai; Yu Haibin; Zhang Jinming; Zhang Peixin; Lin Qiongfang

    1990-01-01

    In recent years, 90 Y has been considered as one of the best radionuclides for tumor radioimmunotherapy when chelated to tumor-associated antibodies. This evaluation is based on the superior properties of this radionuclide (suitable half-life, pure β-ray emitter of intermediate energy, stable daughters, and suitable chemical properties) and because it is available as a radionuclide generator product by decay of its 28a parent 90 Sr. The experimental conditions of 90 Sr- 90 Y generator are described. The elution efficiency of 90 Sr- 90 Y generator reaches 98%. One of the most important problems is the 90 Sr contamination breakthrough from the generator. The level of 90 Sr contamination must be controlled to the clinical standard. The cation exchange resin 732 (100-150 mesh) was successfully used for the separation of 90 Y from 90 Sr. The method used by the authors provides a 90 Y-HAc solution which is very simple and safe for administration to the patients. 90 Y was separated from 90 Sr almost completely, the level of the 90 Sr contamination per 740 MBq 90 Y product was only 0.74 kBq. However the toxicity of 90 Sr is extremely high, the human life-time permissible dose is 74 kBq, then 740 MBq of 90 Y is allowed to be administrated to a patient for 50-100 times

  6. Experimental autoabsorption curve 90Sr in SrCO3. Efficiency calculation to detection of 90Sr, 90Y and 90Sr + 90Y in a beta gas proportional counter

    International Nuclear Information System (INIS)

    Gasco, C.; Alvarez, A.

    1987-01-01

    Strontium-90 has been determined by radiochemical separation techniques in environmental samples. These techniques, of course, cannot separate the two strontium radionuclides from each other of from stable strontium. Consequently the end product of the chemical separation contains all strontium isotopes in SrCO 3 . The beta particules emitted by 90 Sr are absorbed by the SrCO 3 precipitate. This is the main source of error in the activity measurement. It has been prepared sources of 90 Sr in SrCO 3 in order to determinate the counting efficiency and autoabsorption curve. Also detection efficiencies have been calibrated using known activities of 90 Y and equilibrium mixture of 90 Sr+ 90 Y in the same geometry than our samples. The activity of 90 Sr by ingrowth of 90 Y has been calculated by our computer program. (author). 2 figs., 3 refs

  7. Avaliação nutricional e consumo alimentar de pacientes com doença celíaca com e sem transgressão alimentar

    Directory of Open Access Journals (Sweden)

    Cristiana Santos Andreoli

    2013-06-01

    Full Text Available OBJETIVO: Avaliar o estado nutricional e a ingestão de energia e de macronutrientes de pacientes com diagnóstico de doença celíaca que transgrediam ou não a dieta isenta de glúten. MÉTODOS: Foram estudados 63 pacientes com doença celíaca: 34 crianças e 29 adolescentes. Transgressão à dieta isenta de glúten foi caracterizada por meio da dosagem sérica do anticorpo antitransglutaminase tissular recombiante humana. O estado nutricional foi avaliado com base nos escores-Z de peso/idade, estatura/idade e no índice de massa corporal. A ingestão alimentar foi avaliada por meio do inquérito alimentar de 24 horas. RESULTADOS: A transgressão à dieta sem glúten foi constatada em 41,2% das crianças e em 34,5% dos adolescentes. Nas crianças com transgressão alimentar, a média do escore-Z de estatura/idade foi inferior à das crianças do grupo que não transgredia (p=0,024. Todavia, o grupo com transgressão apresentou maior escore-Z do índice de massa corporal em relação aos que não transgrediam (p=0,021. Os adolescentes que não transgrediam apresentaram maior índice de massa corporal quando comparados aos que transgrediam a dieta (p=0,037. Em relação à ingestão alimentar, não se observou diferença estatística entre os grupos. Todavia, cerca de 70,0% das crianças e adolescentes apresentaram consumo de energia acima de 120,0% da recomendação. CONCLUSÃO: As crianças que transgrediam a dieta apresentaram menor escore-Z de estatura/idade e maior escore-Z para índice de massa corporal do que crianças que seguem sem transgressões alimentares. Os adolescentes que não transgrediam a dieta apresentaram maior média de índice de massa corporal quando comparados aos que transgrediam a dieta. Consumo energético elevado foi observado tanto nas crianças quanto nos adolescentes.

  8. Market regulation in Central America and Bit-Energy.CEL as tool for improving the self regulating forces for a liberalised market

    International Nuclear Information System (INIS)

    Reisinger, H.; Reuter, A.; Dulle, H.

    2002-01-01

    establishment of regional spot and contracts market comprising 6 Central American countries; 3. the introduction of a retail market, allowing auto generation for industrial consumers; 4. the introduction of a renewable and rural electrification support scheme; 5. the introduction of the stochastic planning tool Bit-Energy.CEL for making offers for the spot market less predictable and more flexible. The proposed paper will show more details on the history of market liberalisation in Central America, summarise ongoing activities for improving market efficiency and explain in more detail which role Bit-Energy.CEL plays in this process. (author)

  9. Soroprevalência da doença celíaca em crianças e adolescentes com diabetes melito tipo 1 Serum prevalence of celiac disease in children and adolescents with type 1 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Jacqueline Araújo

    2006-06-01

    Full Text Available OBJETIVO: A associação de doença celíaca e diabetes melito já é conhecida há várias décadas. Pode ser encontrada em uma grande proporção de pacientes diabéticos, que geralmente são assintomáticos. O objetivo do estudo foi avaliar a soroprevalência da doença celíaca em crianças e adolescentes com diabetes melito tipo 1. MÉTODOS: Através de um estudo transversal, realizou-se triagem sorológica com anticorpo IgA antitransglutaminase humana em 354 crianças e adolescentes diabéticos, atendidos em ambulatórios de endocrinologia pediátrica de Recife, Pernambuco, no período de janeiro a junho de 2004. RESULTADOS: O antitransglutaminase humana foi positivo em 37/354 pacientes, resultando em soroprevalência de 10,5% (IC95% 7,6-14,2%. Dentre os pacientes soropositivos, houve predomínio do sexo masculino (56,8% em relação ao feminino (43,2%, porém sem significância estatística. O anticorpo antiendomísio foi realizado nos pacientes com antitransglutaminase humana positivo, sendo negativo em 14/37 (37,8% e positivo em 22/37 (59,5%. CONCLUSÕES: A soroprevalência da doença celíaca em crianças e adolescentes diabéticos encontrada em Pernambuco é elevada, sendo comparável à observada em estudos da América do Norte e Europa e menor do que na África, sugerindo que a triagem sorológica para doença celíaca seja realizada em todas as crianças e adolescentes com diabetes melito tipo 1.OBJECTIVE: The association between celiac disease and diabetes mellitus has been known for many decades. This combination can be observed in a large proportion of diabetic patients, who are generally asymptomatic. The objective of this study was to evaluate the seroprevalence of celiac disease in children and adolescents with type 1 diabetes mellitus. METHODS: This was a cross-sectional study employing antibody IgA anti-transglutaminase for the serological screening of 354 diabetic children and adolescents treated at pediatric endocrinology

  10. Pattern changes in quantitative and qualitative markers of hematopoietic stem cells during acute and chronic exposure to Sr"9"0 isotope in cell culture

    International Nuclear Information System (INIS)

    Russu, Yi.Z.; Byil'ko, D.Yi.; Byil'ko, N.M.; Rodyionova, N.K.

    2015-01-01

    To study the condition of stem cells and their immediate progenitors we implemented cell culture methodology in vivo in gel diffusion capsules with subsequent analysis of the colonies and clusters. On the basis of experiments it was established that long-term effects of incorporated "9"0 Sr isotope leads to significant disturbances in the hematopoietic system and in particular, revealing changes in hematological parameters of irradiated animals such as the appearance of circulating progenitor cells in peripheral blood, reducing the colony-forming efficiency of the bone marrow derived progenitor cells, as well as quantitative and qualitative changes in the clones. Indices confirm the connection of the detected effects in individuals exposed to ionizing radiation described in the earlier publications and can serve as basis for developing criteria for the formation of risk groups among people exposed to "9"0 Sr

  11. Measurements of the 169Tm(n,2n)168Tm cross section between 9.0 and 17.5 MeV

    Science.gov (United States)

    Soter, J.; Bhike, Megha; Krishichayan, Fnu; Finch, S. W.; Tornow, W.

    2016-09-01

    Measurements of the 169Tm(n,2n)168Tm cross section have been performed in 0.5 MeV intervals for neutron energies ranging from 9.0 MeV to 17.5 MeV in order to resolve discrepancies in the current literature data. The neutron activation technique was used with 90Zr and 197Au as monitor foils. After irradiation, de-excitation gamma rays were recorded off-line with High-Purity Germanium (HPGE) detectors in TUNL's Low-Background Counting Facility. In addition, data for the 169Tm(n,3n)167Tm reaction have also been obtained from 15.5 MeV to 17.5 MeV. The results of these measurements provide the basis for investigating properties of the interial confinement fusion plasma in deuterium-tritium (DT) capsules at the National Ignition Facility located at Lawrence Livermore National Laboratory.

  12. Determinação da CL90 e TL90 do isolado IBCB66 de Beauveria bassiana (Ascomycetes: Clavicipitaceae para o controle de Rhipicephalus (Boophilus microplus (Acari: Ixodidae Determination of LC 90 and LT 90 of IBCB66 Beauveria bassiana (Ascomycetes: Clavicipitaceae isolate for Rhipicephalus (Boophilus microplus (Acari: Ixodidae control

    Directory of Open Access Journals (Sweden)

    Barci Leila A. G.

    2009-12-01

    Full Text Available O presente trabalho teve por objetivo avaliar a patogenicidade e a virulência do isolado IBCB66 de Beauveria bassiana para larvas de Rhipicephalus (Boophilus microplus. O isolado IBCB66 foi utilizado como padrão, com a finalidade de determinar a CL50 (Concentração Letal, CL90, TL50 (Tempo Letal e TL90. O isolado IBCB66 foi testado em seis concentrações diferentes (5 × 10(6, 10(7, 5 × 10(7, 10(8, 5 × 10(8 e 10(9 para determinar a porcentagem de mortalidade. A mortalidade total de larvas foi observada 18 dias após o início do teste no grupo tratado com 5 × 10(9 conídios.mL-1. A análise de Probit dos dados consignados apontou a CL50 e CL90 concentrações de 3 × 10(7 e 5 × 10(8 conídios.mL-1 e para TL50 e TL90, foram 10 e 16 dias, respectivamente.The objective of this research was to evaluate the pathogenicity and the virulence of the IBCB66 isolate of Beauveria bassiana on infected larvae of Rhipicephalus (Boophilus microplus. The IBCB66 fungus strain was used as standard isolates of B. bassiana against R. (B. microplus larvae. The larval bioassay tests using the IBCB66 isolate were carried out to determine the (Lethal Concentration LC50, LC90, (Lethal Time LT50 and LT90. The IBCB66 fungus strain was tested at six different concentrations (5 × 10(6, 10(7, 5 × 10(7, 10(8, 5 × 10(8 and 10(9 conidia.mL-1 to determine the percentage of larval mortality. In addition, a Probit analysis was also performed. Total larval mortality was observed eighteen days after the beginning of the test in the group treated with 5 × 10(9 conidia.mL-1. The LC50 and LC90 were 3 × 10(7 and 5 × 10(8 respectively and the LT50 and LT90 were 10 and 16 days.

  13. A retrospective review of Y90 synovectomy in Auckland

    International Nuclear Information System (INIS)

    Stamp, L.

    2000-01-01

    Full text: A retrospective review of Yttrium 90 synovectomies carried out from June 1997 to February 1999 in Auckland Hospital to determine outcome from the procedure. Methods: Patients were sent a questionnaire for each treated knee The principal outcome measures assessed in the month prior and the 6 months after the Y90 were pain, swelling, walking distance and overall disability score. Referring doctors were sent a questionnaire to provide information on duration of synovitis and treatment. Outcomes measured were presence of effusion 6 months after the Y90, requirement for intra-articular steroid in the 12 months prior to and after the yttrium and change of therapy due lo ongoing symptoms in the treated knee. Results: 29 patients and 40 knees had Y90 during the study period 86% patients with 87.5% knees responded. Referring doctors for 86% patients accounting for 87.5% knees responded. The procedure was well tolerated with the majority of patients reporting nil-mild discomfort during and in the 24 hours after Y90. 86% knees treated reported improvement with a reduction in pain and swelling Patient global assessment reduced from 3.8 to 2.0 while physician global assessment reduced from 3.9 to 3.0. There was an improvement in walking distance 6 months after Y90 with the number of knees able to walk >1km increasing from 2 to 17 14 of 25 patients required a change in treatment because of ongoing problems in the treated knee and one patient has undergone knee joint replacement One further patient is waiting knee joint replacement The need for intra-articular steroid was reduced with 83%, knees having an average of 2.4 steroid injections in the 12 months prior to Y90 compared to 38% knees having an average of 0.7 steroid injections in the 12 months after Y90. Of the 30 knees reporting Improvement 24 knees are at least 12 months post Y90. Of these 50% were Improved at 12 months. As a result of this audit we continue to offer this procedure in the Auckland area, and

  14. Synergistic role of HSP90α and HSP90β to promote myofibroblast persistence in lung fibrosis.

    Science.gov (United States)

    Bellaye, Pierre-Simon; Shimbori, Chiko; Yanagihara, Toyoshi; Carlson, David A; Hughes, Philip; Upagupta, Chandak; Sato, Seidai; Wheildon, Nolan; Haystead, Timothy; Ask, Kjetil; Kolb, Martin

    2018-02-01

    Idiopathic pulmonary fibrosis (IPF) is a progressive disease of the lung parenchyma, causing significant morbidity through worsening dyspnoea and overall functional decline. IPF is characterised by apoptosis-resistant myofibroblasts, which are a major source for the excessive production of extracellular matrix (ECM) overtaking normal lung tissue. We sought to study the role of heat shock protein (HSP) isoforms HSP90α and HSP90β, whose distinct roles in lung fibrogenesis remain elusive.We determined the level of circulating HSP90α in IPF patients (n=31) and age-matched healthy controls (n=9) by ELISA. The release of HSP90α and HSP90β was evaluated in vitro in primary IPF and control lung fibroblasts and ex vivo after mechanical stretch on fibrotic lung slices from rats receiving adenovector-mediated transforming growth factor-β1.We demonstrate that circulating HSP90α is upregulated in IPF patients in correlation with disease severity. The release of HSP90α is enhanced by the increase in mechanical stress of the fibrotic ECM. This increase in extracellular HSP90α signals through low-density lipoprotein receptor-related protein 1 (LRP1) to promote myofibroblast differentiation and persistence. In parallel, we demonstrate that the intracellular form of HSP90β stabilises LRP1, thus amplifying HSP90α extracellular action.We believe that the specific inhibition of extracellular HSP90α is a promising therapeutic strategy to reduce pro-fibrotic signalling in IPF. Copyright ©ERS 2018.

  15. Country report: Syria. Development of 90Y/90Sr Generator and 90Y Radiopharmaceuticals

    International Nuclear Information System (INIS)

    Yassine, Taufik; Mukhallalati, Ch. Heyam

    2010-01-01

    The aim of this project is to develop a technique for preparation of 90 Sr- 90 Y generator, we have developed a separation technique for isolation of 90 Y from 90 Sr based on using Sr – Spec resin packed in three columns for separation and purification of 90 Sr- 90 Y .The resulting Y90 is used for therapeutic applications. The first part of this project describe a prototype design for the 90 Sr- 90 Y generator in order to get a very accurate method to obtain the minimum possible 90 Sr Breakthrough. 25 mci of 90 Sr was used in the generator 90 Sr- 90 Y and we obtained the elution yield of 90 Y higher than 88%, Also the eluate was used for preparation of several 90 Y radiopharmaceuticals such as 90 YEDTMP and 90 Y-DOTA-HR 3 . And the work is continues to investigate more radiopharmaceuticals applications in the second part of this project such as 90 Y– FHMA. In this part of the co-coordination research programmer, A protocol based on results of this studies was developed to prepare and operate a higher activity generator (50-100mci), the resulting elution yield was approximately 94% of 90 Y . The elute was used in preparation of new 90 Y radiopharmaceuticals. The monoclonal antibodies is still evolving by conjugate Rituximab to The macrocyclic bifunctional chelating agent,(p-SCN-Bn-DOTA)S-2-(4-Isothiocyanatobenzyle)-1,4,7,10- tetraazacyclododecane-tetraaceticacid to obtain the inmunoconjugate DOTA-Rituximub in simple way and then investigating the radio labeling conditions with 90 Y. (author)

  16. Weak signal detection: A discrete window of opportunity for achieving 'Vision 90:90:90'?

    Science.gov (United States)

    Burman, Christopher J; Aphane, Marota; Delobelle, Peter

    2016-01-01

    UNAIDS' Vision 90:90:90 is a call to 'end AIDS'. Developing predictive foresight of the unpredictable changes that this journey will entail could contribute to the ambition of 'ending AIDS'. There are few opportunities for managing unpredictable changes. We introduce 'weak signal detection' as a potential opportunity to fill this void. Combining futures and complexity theory, we reflect on two pilot case studies that involved the Archetype Extraction technique and the SenseMaker(®) Collector(™) tool. Both the piloted techniques have the potentials to surface weak signals--but there is room for improvement. A management response to a complex weak signal requires pattern management, rather than an exclusive focus on behaviour management. Weak signal detection is a window of opportunity to improve resilience to unpredictable changes in the HIV/AIDS landscape that can both reduce the risk that emerges from the changes and increase the visibility of opportunities to exploit the unpredictable changes that could contribute to 'ending AIDS'.

  17. Suppression of 9-cis-epoxycarotenoid dioxygenase, which encodes a key enzyme in abscisic acid biosynthesis, alters fruit texture in transgenic tomato.

    Science.gov (United States)

    Sun, Liang; Sun, Yufei; Zhang, Mei; Wang, Ling; Ren, Jie; Cui, Mengmeng; Wang, Yanping; Ji, Kai; Li, Ping; Li, Qian; Chen, Pei; Dai, Shengjie; Duan, Chaorui; Wu, Yan; Leng, Ping

    2012-01-01

    Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (Solanum lycopersicum) fruit ripening, we suppressed SlNCED1, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress SlNCED1 specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (SlPG), pectin methyl esterase (SlPME), β-galactosidase precursor mRNA (SlTBG), xyloglucan endotransglycosylase (SlXET), endo-1,4-β-cellulose (SlCels), and expansin (SlExp). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (SlPG, SlPME, SlTBG, SlXET, SlCels, and SlExp).

  18. Chemical compositions and antimicrobial activity of twig essential ...

    African Journals Online (AJOL)

    Aghomotsegin

    2016-03-09

    Mar 9, 2016 ... The chemical composition of twig essential oils of Xylopia malayana, Xylopia elliptica and Xylopia fusca were analyzed ... brown or dark green in colors and fragrant. .... extraction used and geographic origin of plant studied.

  19. Progress in radiation chemistry of crown ether extractants used for the solvent extraction of "9"0Sr

    International Nuclear Information System (INIS)

    Peng Jing; Yu Chuhong; Cui Zhenpeng; Zhai Maolin

    2011-01-01

    The separation of the long-lived fission products from dissolved nuclear fuel could improve the safe disposal of high-level nuclear wastes and reduce their threaten to human being and environment. Since the extractant system will be exposed to high radiation environment during the solvent extraction of long-lived fission products. The understanding of radiation chemistry of extractants is very important for practical design of extractant system. The radiation chemistry of crown ether systems proposed for use in the solvent extraction of one of fission products "9"0Sr were reviewed based on the study on the radiation stability and radiolysis mechanism of crown ether system. Finally some challenges were suggested. (authors)

  20. Linseed Dietary Fibers Reduce Apparent Digestibility of Energy and Fat and Weight Gain in Growing Rats

    Directory of Open Access Journals (Sweden)

    Arne Astrup

    2013-08-01

    Full Text Available Dietary fibers (DF may affect energy balance, an effect often ascribed to the viscous nature of some water soluble DF, which affect luminal viscosity and thus multiple physiological processes. We have tested the hypothesis that viscous linseed DF reduce apparent nutrient digestibility, and limit weight gain, in a randomized feeding trial where 60 male, growing, Wistar rats, with an initial weight of ~200 g, were fed different diets (n = 10 per group: low DF control (C, 5% DF from cellulose (5-CEL, CEL + 5% DF from whole (5-WL or ground linseed (5-GL, CEL + 5% DF from linseed DF extract (5-LDF, and CEL + 10% DF from linseed DF extract (10-LDF. Diets were provided ad libitum for 21 days. Feed intake and faecal output were measured during days 17–21. Faecal fat excretion increased with increasing DF content and was highest in the 10-LDF group. Apparent fat digestibility was highest with the C diet (94.9% ± 0.8% and lowest (74.3% ± 0.6% with the 10-LDF diet, and decreased in a non-linear manner with increasing DF (p < 0.001. Apparent fat digestibility also decreased with increased accessibility of DF (5-WL vs. 5-GL and when the proportion of viscous DF increased (5-GL vs. 5-LDF. The 10-LDF resulted in a lower final body weight (258 ± 6.2 g compared to C (282 ± 5.9 g, 5-CEL (281 ± 5.9 g, and 5-WL (285 ± 5.9 g (p < 0.05. The 10-LDF diet reduced body fat compared to 5-CEL (p < 0.01. In conclusion, DF extracted from linseed reduced apparent energy and fat digestibility and resulted in restriction of body weight gain in growing rats.

  1. Comparación de tres leguminosas arbóreas sembradas en un sustrato alcalino durante el período de aviveramiento. II. Patrón de crecimiento y distribución de la biomasa Comparison of three tree legumes planted on an alkaline substratum during the nursery stage. II. Growth and biomass distribution pattern

    Directory of Open Access Journals (Sweden)

    María G Medina¹

    2011-06-01

    Full Text Available Se desarrolló un experimento en el estado Trujillo, Venezuela, con el objetivo de comparar el patrón de crecimiento y la distribución de biomasa de Albizia lebbeck, Leucaena leucocephala (ecotipo Trujillo y Erythrina fusca durante 18 semanas en vivero. Las variables estudiadas fueron: tasa de crecimiento en función de la altura (TCA y de la distribución de la MS (TCMS, así como el porcentaje de materia seca (MS y el peso seco por planta de las fracciones (hojas, tallo, raíz. Se utilizó un diseño completamente aleatorizado para mediciones repetidas en el tiempo y diez réplicas por especie. Erythrina presentó la mayor TCA y TCMS promedio (3,7 mm/día y 94,8 mg/día, seguida por leucaena (3,1 y 81,3 y, por último, albizia (1,2 mm/día y 53,1 mg/día, respectivamente. Independientemente de la especie, el mayor porcentaje de MS promedio se observó en las raíces (31,9, seguido por el tallo (28,0 y las hojas (25,4. Se observó una mayor distribución de MS por planta en las hojas (3,9 g y la raíz (3,1 g, comparados con el tallo (2,9 g. Se concluye que, para estas condiciones de aviveramiento, E. fusca presentó los mejores resultados; mientras que A. lebbeck necesitaría más tiempo para alcanzar las características deseables para su trasplante a campo.A trial was conducted in the Trujillo state, Venezuela, in order to compare the growth and biomass distribution pattern of Albizia lebbeck, Leucaena leucocephala (Trujillo ecotype and Erythrina fusca for 18 weeks in nursery. The studied variables were: growth rate related to height (GRH and DM distribution (GRDM, as well as dry matter percentage (DM and dry weight per plant of the fractions (leaves, stem, root. A completely randomized design for measurements repeated in time and ten replications per species were used. E. fusca showed the highest average GRH and GRDM (3,7 mm/day and 94,8 mg/day, followed by L. leucocephala (3,1 and 81,3 and, lastly, A. lebbeck (1,2 mm/day and 53,1 mg

  2. Development of 90Sr-90Y generators

    International Nuclear Information System (INIS)

    Barrio, Graciela

    2007-01-01

    Yttrium-90 is a radioisotope of great interest in the field of Nuclear Medicine. It is considered one of the most important and most used radionuclides for radioimmunotherapeutical applications, especially promising for the treatment of certain types of cancer. Another important application of 90 Y is radio synovectomy. This radionuclide has a half-life of 64 hours, emits long range beta particles (maximum energy of 2.3 MeV) and decays, without intermediate nuclides, to a stable daughter. 90 Y may be obtained carrier-free, generated by the decay of its parent 90 Sr (half-life=28 years). 90 Sr is a product from uranium fission, and due to its long half-life, can be indefinitely used, which is certainly advantageous. It is present in great amounts, and needs to be processed and purified in order to be used as raw material for the generators. Generators of 90 Sr- 90 Y may thus be used during various months, due to 90 Sr long half-life. Several methods for the separation of 90 Y from 90 Sr by solvent extraction and ion exchange have been reported in literature. Thanks to its simplicity, ion exchange techniques have been more commonly used for this generator system. The main objective of this work was to develop a methodology for the preparation of 90 Sr- 90 Y generators, using cationic exchange resins. In such method, 90 Sr is strongly adsorbed in the resin and 90 Y is eluted by a 0.003 M EDTA solution. According to the quality control carried out, results showed that elution yields are greater than 65%, thus confirming the efficiency of the separation method used.

  3. Gradiente de riesgo genético HLA-DQ para diabetes tipo 1 y enfermedad celíaca en el noroeste de México

    OpenAIRE

    Mejía-León, M.E.; Ruiz-Dyck, K.M.; Calderón de la Barca, A.M.

    2015-01-01

    Antecedentes: La diabetes tipo 1 (DT1) y la enfermedad celíaca (EC) son 2 enfermedades autoinmunes frecuentes en la infancia y comparten su predisposición genética (HLA-DQ2 y DQ8). La prevalencia de ambas se ha incrementado en el mundo. En el estado de Sonora (15 habitantes/km2), se desconoce información sobre su riesgo genético o la distribución de los alelos asociados en la población general. Objetivo: Comparar la frecuencia alélica HLA-DQ de una muestra representativa de recién nacidos ...

  4. Celiac artery in New Zealand rabbit: anatomical study of its origin and arrangement for experimental research and surgical practice Artéria celíaca em coelhos Nova Zelândia: estudo anatômico de sua origem e arranjo para a pesquisa experimental e a prática cirúrgica

    Directory of Open Access Journals (Sweden)

    Marcelo Abidu-Figueiredo

    2008-05-01

    trajeto. Foram utilizados 30 coelhos, 13 machos e 17 fêmeas, pesando em media 2,5 kg e apresentando comprimento rostro-sacral em torno de 40cm. A artéria aorta torácica foi canulada e através da mesma foi feita à fixação com solução de formaldeído a 10% e repleções vasculares com solução de Petrolátex S65 corado. A artéria celíaca e suas ramificações proximais foram dissecadas ao longo do seu percurso, registrando com auxílio de um paquímetro seu comprimento e sua esqueletopia. A artéria celíaca teve sua emergência de forma única diretamente da artéria aorta abdominal em todos os animais dissecados. Emitiu inicialmente a artéria lienal e a seguir a artéria gástrica esquerda que se continuou como hepática em todos os 30 animais. A artéria celíaca teve sua origem entre a 12ªe 13ª vértebra torácica em 11 animais (36,7 %, na 13ª vértebra torácica em 6 (20 %, entre a 13ª vértebra torácica e a 1ª vértebra lombar em 12 (40 % e na 1ª vértebra lombar em apenas 1 animal (3,3%. O comprimento médio da artéria celíaca foi de 0,5cm. Não foi observada relação entre o comprimento da artéria celíaca e o comprimento rostro-sacral dos coelhos. O número de artérias gástricas esquerdas, ramificações principais da artéria lienal, bem como a origem da artéria celíaca independeram do sexo do animal.

  5. Synthesis of bis-cyclopentadienyl compounds with the 9.9-fluorenylidene bridge. The crystal and molecular structure of [μ-9.9-Flu(η5-Cp)2]ZrCl2

    International Nuclear Information System (INIS)

    Ivchenko, N.B.; Ivchenko, P.V.; Nifant'ev, I.Eh.; Bagrov, V.V.; Kuz'mina, L.G.

    2000-01-01

    Effect method for synthesis of ansa-zirconocenes containing short 9,9-fluorenylidene bridges between cyclopentadienyl ligands of zirconium complex was developed. Crystal and molecular structures of one of the complexes synthesized of the composition [μ-9,9-Flu(η 5 -Cp) 2 ]ZrCl 2 (Flu = fluorenylidene, Cp = cydopentadiene) were determined by the method of X-ray diffraction analysis. The compound has a monoclinic cell with the following parameters: a = 11.386 (6), b = 13.555 (5), c = 15.947 (1)A; α = 90 deg, β = 102.59 deg, γ = 90 deg; sp.gr. P2 1 /n, Z 4; d calcld. = 1.512 g/cm 3 [ru

  6. A special mini-extrapolation chamber for calibration of 90Sr+90Y sources

    International Nuclear Information System (INIS)

    Oliveira, Mercia L; Caldas, Linda V E

    2005-01-01

    90 Sr+ 90 Y applicators are commonly utilized in brachytherapy, including ophthalmic procedures. The recommended instruments for the calibration of these applicators are extrapolation chambers, which are ionization chambers that allow the variation of their sensitive volume. Using the extrapolation method, the absorbed dose rate at the applicator surface can be determined. The aim of the present work was to develop a mini-extrapolation chamber for the calibration of 90 Sr+ 90 Y beta ray applicators. The developed mini-chamber has a 3.0 cm outer diameter and is 11.3 cm in length. An aluminized polyester foil is used as the entrance window while the collecting electrode is made of graphited polymethylmethacrylate. This mini-chamber was tested in 90 Sr+ 90 Y radiation beams from a beta particle check source and with a plane ophthalmic applicator, showing adequate results

  7. Evaluación de la validez y fiabilidad de un cuestionario específico de calidad de vida relacionada con la salud en celíacos de 8-18 años.

    OpenAIRE

    Barrio Torres, Josefa

    2016-01-01

    Tesis Doctoral leída en la Universidad Rey Juan Carlos de Madrid en 2015. Directores de la Tesis: Angel Gil Miguel, Enriqueta Román Riechmann y Mª Luz Cilleruelo La enfermedad celíaca (EC) es una enfermedad sistémica mediada por el sistema inmune y ocasionada por el gluten y las prolaminas relacionadas que se produce en individuos genéticamente predispuestos. Cursa con una atrofia de las vellosidades intestinales de la mucosa del intestino delgado superior y como consecuencia s...

  8. Plant carbohydrate binding module enhances activity of hybrid microbial cellulase enzyme

    Directory of Open Access Journals (Sweden)

    Caitlin Siobhan Byrt

    2012-11-01

    Full Text Available A synthetic, highly active cellulase enzyme suitable for in planta production may be a valuable tool for biotechnological approaches to develop transgenic biofuel crops with improved digestibility. Here, we demonstrate that the addition of a plant derived carbohydrate binding module (CBM to a synthetic glycosyl hydrolase (GH improved the activity of the hydrolase in releasing sugar from plant biomass. A CEL-HYB1-CBM enzyme was generated by fusing a hybrid microbial cellulase, CEL-HYB1, with the carbohydrate-binding module (CBM of the tomato (Solanum lycopersicum SlCel9C1 cellulase. CEL-HYB1 and CEL-HYB1-CBM enzymes were produced in vitro using Pichia pastoris and the activity of these enzymes was tested using CMC, MUC and native crystalline cellulose assays. The presence of the CBM substantially improved the endo-glucanase activity of CEL-HYB1, especially against the native crystalline cellulose encountered in Sorghum plant cell walls. These results indicate that addition of an endogenous plant derived CBM to cellulase enzymes may enhance hydrolytic activity.

  9. Development of {sup 90}Sr-{sup 90}Y generators; Desenvolvimento de geradores de {sup 90}Sr-{sup 90}Y

    Energy Technology Data Exchange (ETDEWEB)

    Barrio, Graciela, E-mail: gracielabarrio@gmail.co [Pontificia Univ. Catolica de Sao Paulo (PUC-SP), SP (Brazil). Dept. de Fisica; Osso Junior, Joao Alberto, E-mail: jaossoj@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2007-07-01

    Yttrium-90 is a radioisotope of great interest in the field of Nuclear Medicine. It is considered one of the most important and most used radionuclides for radioimmunotherapeutical applications, especially promising for the treatment of certain types of cancer. Another important application of {sup 90}Y is radio synovectomy. This radionuclide has a half-life of 64 hours, emits long range beta particles (maximum energy of 2.3 MeV) and decays, without intermediate nuclides, to a stable daughter. {sup 90}Y may be obtained carrier-free, generated by the decay of its parent {sup 90}Sr (half-life=28 years). {sup 90}Sr is a product from uranium fission, and due to its long half-life, can be indefinitely used, which is certainly advantageous. It is present in great amounts, and needs to be processed and purified in order to be used as raw material for the generators. Generators of {sup 90}Sr-{sup 90}Y may thus be used during various months, due to {sup 90}Sr long half-life. Several methods for the separation of {sup 90}Y from {sup 90}Sr by solvent extraction and ion exchange have been reported in literature. Thanks to its simplicity, ion exchange techniques have been more commonly used for this generator system. The main objective of this work was to develop a methodology for the preparation of {sup 90}Sr-{sup 90}Y generators, using cationic exchange resins. In such method, {sup 90}Sr is strongly adsorbed in the resin and {sup 90}Y is eluted by a 0.003 M EDTA solution. According to the quality control carried out, results showed that elution yields are greater than 65%, thus confirming the efficiency of the separation method used.

  10. National responses to global health targets: exploring policy transfer in the context of the UNAIDS '90-90-90' treatment targets in Ghana and Uganda.

    Science.gov (United States)

    McRobie, Ellen; Matovu, Fred; Nanyiti, Aisha; Nonvignon, Justice; Abankwah, Daniel Nana Yaw; Case, Kelsey K; Hallett, Timothy B; Hanefeld, Johanna; Conteh, Lesong

    2018-01-01

    Global health organizations frequently set disease-specific targets with the goal of eliciting adoption at the national-level; consideration of the influence of target setting on national policies, programme and health budgets is of benefit to those setting targets and those intended to respond. In 2014, the Joint United Nations Programme on HIV/AIDS set 'ambitious' treatment targets for country adoption: 90% of HIV-positive persons should know their status; 90% of those on treatment; 90% of those achieving viral suppression. Using case studies from Ghana and Uganda, we explore how the target and its associated policy content have been adopted at the national level. That is whether adoption is in rhetoric only or supported by programme, policy or budgetary changes. We review 23 (14 from Ghana, 9 from Uganda) national policy, operational and strategic documents for the HIV response and assess commitments to '90-90-90'. In-person semi-structured interviews were conducted with purposively sampled key informants (17 in Ghana, 20 in Uganda) involved in programme-planning and resource allocation within HIV to gain insight into factors facilitating adoption of 90-90-90. Interviews were transcribed and analysed thematically, inductively and deductively, guided by pre-existing policy theories, including Dolowitz and Marsh's policy transfer framework to describe features of the transfer and the Global Health Advocacy and Policy Project framework to explain observations. Regardless of notable resource constraints, transfer of the 90-90-90 targets was evident beyond rhetoric with substantial shifts in policy and programme activities. In both countries, there was evidence of attempts to minimize resource constraints by seeking programme efficiencies, prioritization of programme activities and devising domestic financing mechanisms; however, significant resource gaps persist. An effective health network, comprised of global and local actors, mediated the adoption and adaptation

  11. Phytoplanktonic composition of three cultivation systems used in Litopenaeus vannamei (BOONE, 1931 marine shrimp farms = Composição fitoplanctônica em três sistemas de cultivo do camarão marinho Litopenaeus vannamei (BOONE, 1931

    Directory of Open Access Journals (Sweden)

    Michelle Pereira Melo

    2010-07-01

    Full Text Available The aim of this work is to assess the different compositions of phytoplankton in three cultivation systems of marine shrimps Litopenaeus vannamei (BOONE, 1931, denominated as organic, intensive and semi intensive. The samples were done fortnightly, when phytoplankton was collected by a net for phytoplankton, 65 ƒÊm mesh, being then filtrated in a total volume of water of 100 L, and preserved in formaldehyde solution at 4% and identified according to the methodology of Cordeiro et al. (1997. The results show that the densities of Diatoms were of 16.65, 10.47 and 7.57 cel. 103 mL-1 for the organic, intensive and semi intensive cultivations, respectively. As for cyanobacteria, the average figures were 42.06 cel. 103 mL-1 forsemi intensive 17.27 cel. 103 mL-1, in the intensive cultivation and 6.11 cel. 103 mL-1 for the organic cultivation system. The dinoflagellates had the highest cellular density in the phytoplankton community analyzed with 61.9 cel. 103 mL-1 in the intensive cultivation, 0.33 and 0.03 cel. 103 mL-1 for both semi intensive and organic cultivation systems respectively. Euglenas presented the results of 4.98 and 14.86 cel. 103 mL-1 only for semi intensive and intensive cultivations. It was then concluded that all cultivations presented average rates below recommended for such studied systems.Conduziu-se esse trabalho com o objetivo de avaliar as diferentes composicoes fitoplanctonicas em tres sistemas de cultivo para o camarao marinho Litopenaeus vannamei (BOONE, 1931, denominados de organico, intensivo e semiintensivo. As amostragens foram realizadas quinzenalmente, onde o fitoplancton foi coletado atraves de uma rede de plancton, com malha de 65 ƒÊm, sendo filtrado um volume de agua total de 100 litros, que foram preservadas em solucao de formol a 4% e identificadas segundo a metodologia de Cordeiro et al. (1997. Os resultados mostram que as densidades de diatomaceas foram de 16,65; 10,47 e 7,57 cel. 103 mL-1, respectivamente

  12. Substituting Both the N-Terminal and "Cord" Regions of a Xylanase from Aspergillus oryzae to Improve Its Temperature Characteristics.

    Science.gov (United States)

    Li, Chuang; Li, Jianfang; Wang, Rui; Li, Xueqing; Li, Jinping; Deng, Chao; Wu, Minchen

    2018-02-06

    To improve the temperature characteristics of AoXyn11A, a mesophilic glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae CICC40186, its N-terminal and "cord" regions were selected to be substituted by means of the computer-aided analysis and calculation. In brief, one mutant, named ATX11A 41 , possessing the lowest root-mean-square deviation (RMSD) value was designed based on the molecular dynamics (MD) simulation by substituting the N-terminal 41 amino acids of AoXyn11A with the corresponding 42 ones of pXYL11, a thermophilic GHF11 xylanase from Thermobifida fusca. On the basis of the primary structure alignment of pXYL11 with ATX11A 41 (or AoXyn11A), another mutant, named ATX11A 41/cord , was designed by substituting the cord region ( 93 GTYNPGSGG 101 ) of ATX11A 41 with the corresponding one ( 93 GTYRPTG 99 ) of pXYL11. Both mutant-encoding genes, ATx11A 41 and ATx11A 41/cord , were constructed as designed theoretically by a megaprimer PCR technique and were expressed in Pichia pastoris GS115. The specific activities of recombinant (re) AoXyn11A, ATX11A 41 , and ATX11A 41/cord were 2916.7, 2667.6, and 2457.0 U/mg, respectively. The analysis of temperature characteristics displayed that the temperature optimum (T opt ) of reATX11A 41 or reATX11A 41/cord was 65 °C, which was 15 °C higher than that of reAoXyn11A. The thermal inactivation half-life (t 1/2 ) values of reATX11A 41 and reATX11A 41/cord at 60 °C were 55 and 83 min, respectively, whereas that of reAoXyn11A was only 18 min at 50 °C. The melting temperature (T m ) values of reAoXyn11A, reATX11A 41 , and reATX11A 41/cord were 54.2, 66.7, and 71.9 °C, respectively. In conclusion, the above findings indicated that the substitution of both the N-terminal and cord regions of a mesophilic AoXyn11A greatly contributed to its improved temperature characteristics.

  13. Overlapping and distinct roles of AKIN10 and FUSCA3 in ABA and sugar signaling during seed germination.

    Science.gov (United States)

    Tsai, Allen Yi-Lun; Gazzarrini, Sonia

    2012-10-01

    The Arabidopsis B3-domain transcription factor FUSCA3 (FUS3) is a master regulator of seed maturation and also a central modulator of hormonal responses during late embryogenesis and germination. Recently, we have identified AKIN10, the Arabidopsis ortholog of Snf1 (Sucrose Non-Fermenting-1)-Related Kinase1 (SnRK1), as a FUS3-interacting protein. We demonstrated that AKIN10 physically interacts with and phosphorylates FUS3 at its N-terminal region, and genetically interacts with FUS3 to regulate developmental phase transition and lateral organ growth. Snf1/AMPK/SnRK1 kinases are important sensors of the cellular energy level, and they are activated in response to starvation and cellular stress. Here we present findings that indicate FUS3 and AKIN10 functionally overlap in ABA signaling, but play different roles in sugar responses during germination. Seeds overexpressing FUS3 and AKIN10 both display ABA-hypersensitivity and delayed germination. The latter is partly dependent on de novo ABA synthesis in both genotypes, as delayed germination can be partially rescued by the ABA biosynthesis inhibitor, fluridone. However, seeds and seedlings overexpressing FUS3 and AKIN10 show different sugar responses. AKIN10-overexpressing seeds and seedlings are hypersensitive to glucose, while those overexpressing FUS3 display overall defects in osmotic stress, primarily during seedling growth, as they show increased sensitivity toward sorbitol and glucose. Hypersensitivity to sugar and/or osmotic stress during germination are partly dependent on de novo ABA synthesis for both genotypes, although are likely to act through distinct pathways. This data suggests that AKIN10 and FUS3 both act as positive regulators of seed responses to ABA, and that AKIN10 regulates sugar signaling while FUS3 mediates osmotic stress responses.

  14. Engineering ionic liquid-tolerant cellulases for biofuels production.

    Science.gov (United States)

    Wolski, Paul W; Dana, Craig M; Clark, Douglas S; Blanch, Harvey W

    2016-04-01

    Dissolution of lignocellulosic biomass in certain ionic liquids (ILs) can provide an effective pretreatment prior to enzymatic saccharification of cellulose for biofuels production. Toward the goal of combining pretreatment and enzymatic hydrolysis, we evolved enzyme variants of Talaromyces emersonii Cel7A to be more active and stable than wild-type T. emersonii Cel7A or Trichoderma reesei Cel7A in aqueous-IL solutions (up to 43% (w/w) 1,3-dimethylimdazolium dimethylphosphate and 20% (w/w) 1-ethyl-3-methylimidazolium acetate). In general, greater enzyme stability in buffer at elevated temperature corresponded to greater stability in aqueous-ILs. Post-translational modification of the N-terminal glutamine residue to pyroglutamate via glutaminyl cyclase enhanced the stability of T. emersonii Cel7A and variants. Differential scanning calorimetry revealed an increase in melting temperature of 1.9-3.9°C for the variant 1M10 over the wild-type T. emersonii Cel7A in aqueous buffer and in an IL-aqueous mixture. We observed this increase both with and without glutaminyl cyclase treatment of the enzymes. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  15. Tohoku's earthquake of Friday March 11, 2011 (5:46 UT), magnitude 9.0, off Honshu island (Japan); Seisme de Tohoku au large de l'Ile d'Honshu (Japon) du vendredi 11 mars 2011 (5h46 TU) Magnitude = 9,0

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-01

    On Friday March 11, 2011, at 5:46 UT (2:46 PM local time), a magnitude 9.0 earthquake took place at 80 km east of Honshu island (Japan). The earthquake generated a tsunami which led to the loss of the cooling systems of the Fukushima Dai-ichi and Fukushima Daini power plants. This paper describes the seismo-tectonic and historical seismic context of the Japan archipelago and the first analyses of the Tohoku earthquake impact: magnitudes of first shock and of aftershocks, impact on nuclear facilities (maximum acceleration values detected with respect to design basis values, subsidence of coastal areas and submersion of power plant platforms). (J.S.)

  16. Purification and characterization of a salt-tolerant cellulase from the mangrove oyster, Crassostrea rivularis.

    Science.gov (United States)

    An, Tianchen; Dong, Zhu; Lv, Junchen; Liu, Yujun; Wang, Manchuriga; Wei, Shuangshuang; Song, Yanting; Zhang, Yingxia; Deng, Shiming

    2015-04-01

    A cellulase with wide range of pH resistance and high salt tolerance was isolated from the digestive gland of the oyster Crassostrea rivularis living in mangrove forests. The 27 kDa cellulase named as CrCel was purified 40.6 folds by anion exchange chromatography and extraction from the gel after non-reducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The specific activity of the purified cellulase was 23.4 U/mg against carboxymethyl cellulose (CMC). The N-terminal amino acid sequence of CrCel was determined to be NQKCQANSRV. CrCel preferably hydrolyzes β-1,4-glucosidic bonds in the amorphous parts of cellulose materials and displays degradation activity toward xylan. The Km and Vmax values of CrCel for CMC were determined to be 2.1% ± 0.4% and 73.5 ± 3.3 U mg(-1), respectively. The optimal pH value and temperature of CrCel were 5.5 and 40°C, respectively. The enzyme was stable in a wide range of pH, retaining over 60% activity after incubation for 80 min in the pH range of 3.0-9.0. In addition, CrCel showed remarkable tolerance to salt and remained active at high NaCl concentrations, but also retained over 70% activity after incubation in 0.5-2 M NaCl for up to 24 h. On the basis of the N-terminal sequence alignment and its similar properties to other animal cellulases, CrCel was regarded as a member of glycosyl hydrolase family 45 β-1,4-glucanases. CrCel is the first reported cellulase isolated from mangrove invertebrates, which suggests that it may participate in the assimilation of cellulolytic materials derived from the food sources of the oyster and contribute to the consumption of mangrove primary production. The unique properties of this enzyme make it a potential candidate for further industrial application. © The Author 2015. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

  17. Thermostable cellulase from a thermomonospora gene

    Science.gov (United States)

    Wilson, D.B.; Walker, L.P.; Zhang, S.

    1997-10-14

    The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity. 3 figs.

  18. IRONY IN THE POEMS OF CAN YÜCEL CAN YÜCEL’İN ŞİİRLERİNDE İRONİ

    Directory of Open Access Journals (Sweden)

    Soner AKPINAR

    2010-04-01

    Full Text Available Can Yücel, who started to contribute to literature in 1950 with his book “Yazma” , has attracted the attention of the poetry environment especially after 1965, after a 15-20 year of search period. The poet tried to approach the events (incidents and facts with a socialist point of view in his poems in which he put forward swearing, slang and obscenity. One of the reflection forms of his social sensitivity and critical point of view – within the effect of his perception of life – is lampooning. Irony in this respect is the part of lampooning, in addition it’s a vehicle for the poet to locate himself against “nonsense” . In this study the significance of irony in the structure of Can Yücel’s poems is going to be evaluated via examples. 1950 yılında yayımladığı “Yazma” adlı şiir kitabıyla edebiyat dünyasına giren Can Yücel, 15-20 senelik bir arayış devresinin ardından özellikle 1965’ten sonra şiir kamuoyunun dikkatini çekmiştir. Şair, yüzey yapıda küfür, argo ve müstehcenliğin öne çıktığı şiirlerinde toplumcu bir bakış açısıyla olay ve olgulara yaklaşmaya çalışmıştır. Onun toplumsal duyarlılığının, eleştirel bakış açısının yansıma biçimlerinden birisi -yaşam algısının da etkisiyle- taşlama, yergidir. İroni de bu anlamda taşlamanın bir parçası olmakla birlikte, şairin “saçma” karşısında kendisini konumlandırmasına yarayan bir araçtır. Bu incelemede ironinin Can Yücel’in şiirlerinin yapısı içindeki önemi, örnekler üzerinden değerlendirilmeye çalışılacaktır.

  19. Feedback about Earthquake Early Warning: Questionnaire survey after the 2011 Tohoku Earthquake (Mw9.0)

    Science.gov (United States)

    Nakamura, M.; Hoshiba, M.; Matsui, M.; Hayashimoto, N.; Wakayama, A.

    2013-05-01

    We will report the results of a questionnaire survey on Earthquake Early Warning (EEW), conducted by the Japan Meteorological Agency (JMA) in February 2012, approximately one year after the 2011 off the Pacific coast of Tohoku Earthquake (Mw9.0). In the questionnaire survey, which is based on the performance of the 5-year operation of EEW, the respondents were asked how they obtained EEW, how they reacted to EEW and how useful they considered EEW as a safety measure against strong ground shaking. Respondents numbered 817 in the Tohoku district survey and 2,000 in the nationwide survey. Most respondents received EEW messages from TV or cell phone broadcast mail service. Most respondents took some actions in the Tohoku district (74 percent) and nationwide (54 percent); 16 and 17 percent, respectively, tried to take action but could not; and 10 and 29 percent, respectively, did nothing. More than 90 and 80 percent of respondents thought EEW was useful in the Tohoku district and nationwide, respectively. Many people stated that EEW helped them prepare for strong shaking, even if they did not actually take specific actions. The percentage of respondents evaluating EEW to be useful was larger among Tohoku than nationwide. Likewise, the percentage of people who were able to take useful actions was larger in the Tohoku than nationwide. The difference may be attributed to the degree of experience of EEW that had been frequently issued particularly to the Tohoku district since March the 11th 2011. The benefit of the EEW system was recognized both as a trigger of taking actual actions and as an aid to mental preparedness before strong jolts began. Most people considered that the EEW system was useful despite of some false alarms. Although it is necessary to improve the EEW system to reduce false alarms and make the predictions more precise, the results of this survey should be encouraging to the community of promoting and researching EEW.

  20. Peptide Receptor Radionuclide Therapy with ''9''0Y DOTA TATE - First Results

    International Nuclear Information System (INIS)

    Artiko, V.; Sobic-Saranovic, D.; Petrovic, N.; Damjanovic, S.; Obradovic, V.

    2009-01-01

    Aim: The aim of this work is presentation of the preliminary results of the therapy of NETs with 90 Y DOTA TATE. Patients and methods: We investigated 15 patients with various neuroendocrine tumors. In all of them, together with other laboratory analyses and imaging methods, scintigraphy with somatostatin analogues was performed (in 3 with 111 In Octreoscan and in the other 4 with 99m Tc Tektrotyd) and high tumor uptake observed. The therapy was performed with 2-4,5 GBq 90 Y DOTA TATE per patient per one cycle, in the slow infusion in the physiological liquid (150 ml/15 min).Between the cycles, there was a time delay of 6-8 weeks. 30 minutes before the therapy, patients began receiving the infusion of amino acids (arginine and lysine) which lasted 4h. Before that, all therapies with somatostatin analogues were withdrawn. 24h-96h after the therapy, ''bremsstrahlung'' whole body imaging, SPECT and particular planar images were performed with gamma camera. Results: Analysis of the ''bremsstrahlung'' images showed uptake of the radiopharmaceutical in the liver, but the most of the activity was observed in the regions of the ''hot spots'' registered with previous 99m Tc Tektrotyd and 111 In Octreoscan images. According to our results, after the therapy, in two patients occurred progressive disease (PD), in seven stable disease (SD), and in six partial remission (PR). Up to now, there were no major clinical side effects hepatic function. Transient pancytopenia occurred in two patients, and impairment of kidney function in one. Conclusion: In spite of insufficient data, beneficial effects on clinical symptoms, hormone production and tumor proliferation were found, without major clinical side effects. Thus, according to preliminary results, treatment with 90 Y DOTA TATE is feasible method and might be useful for the management of patients with inoperable or disseminated neuroendocrine tumors. (author)

  1. Preventive but Not Curative Efficacy of Celecoxib on Bladder Carcinogenesis in a Rat Model

    Directory of Open Access Journals (Sweden)

    José Sereno

    2010-01-01

    Full Text Available To evaluate the effect of a cyclooxygenase 2 inhibitor, celecoxib (CEL, on bladder cancer inhibition in a rat model, when used as preventive versus as curative treatment. The study comprised 52 male Wistar rats, divided in 5 groups, during a 20-week protocol: control: vehicle, carcinogen: 0.05% of N-butyl-N-(4-hydroxybutyl nitrosamine (BBN, CEL: 10 mg/kg/day of the selective COX-2 inhibitor Celebrex, preventive CEL (CEL+BBN-P, and curative CEL (BBN+CEL-C groups. Although tumor growth was markedly inhibited by the preventive application of CEL, it was even aggravated by the curative treatment. The incidence of gross bladder carcinoma was: control 0/8(0%, BBN 13/20(65%, CEL 0/8(0%, CEL+BBN-P 1/8(12.5%, and BBN+CEL-C 6/8(75%. The number and volume of carcinomas were significantly lower in the CEL+BBN-P versus BBN, accompanied by an ample reduction in hyperplasia, dysplasia, and papillary tumors as well as COX-2 immunostaining. In spite of the reduction of tumor volumes in the curative BBN+CEL-C group, tumor malignancy was augmented. An anti-inflammatory and antioxidant profile was encountered only in the group under preventive treatment. In conclusion, preventive, but not curative, celecoxib treatment promoted a striking inhibitory effect on bladder cancer development, reinforcing the potential role of chemopreventive strategies based on cyclooxygenase 2 inhibition.

  2. Histological Comparison of Kidney Tissue Following Radioembolization with Yttrium-90 Resin Microspheres and Embolization with Bland Microspheres

    International Nuclear Information System (INIS)

    Silva, Suresh de; Mackie, Simon; Aslan, Peter; Cade, David; Delprado, Warick

    2016-01-01

    BackgroundIntra-arterial brachytherapy with yttrium-90 ("9"0Y) resin microspheres (radioembolization) is a procedure to selectively deliver high-dose radiation to tumors. The purpose of this research was to compare the radioembolic effect of "9"0Y-radioembolization versus the embolic effect of bland microspheres in the porcine kidney model.MethodsIn each of six pigs, ~25–33 % of the kidney volume was embolized with "9"0Y resin microspheres and an equivalent number of bland microspheres in the contralateral kidney. Kidney volume was estimated visually from contrast-enhanced fluoroscopy imaging. Morphologic and histologic analysis was performed 8–9 weeks after the procedure to assess the locations of the microspheres and extent of tissue necrosis from "9"0Y-radioembolization and bland embolization. A semi-quantified evaluation of the non-acute peri-particle and perivascular tissue reaction was conducted. All guidelines for the care and use of animals were followed.ResultsKidneys embolized with "9"0Y-radioembolization decreased in mass by 30–70 % versus the contralateral kidney embolized with bland microspheres. These kidneys showed significant necrosis/fibrosis, avascularization, and glomerular atrophy in the immediate vicinity of the "9"0Y resin microspheres. By contrast, glomerular changes were not observed, even with clusters of bland microspheres in afferent arterioles. Evidence of a foreign body reaction was recorded in some kidneys with bland microspheres, and subcapsular scarring/infarction only with the highest load (4.96 × 10"6) of bland microspheres.ConclusionThis study showed that radioembolization with "9"0Y resin microspheres produces localized necrosis/fibrosis and loss of kidney mass in a porcine kidney model. This result supports the study of "9"0Y resin microspheres for the localized treatment of kidney tumors.

  3. Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA

    Science.gov (United States)

    Sun, Liang; Sun, Yufei; Zhang, Mei; Wang, Ling; Ren, Jie; Cui, Mengmeng; Wang, Yanping; Ji, Kai; Li, Ping; Li, Qian; Chen, Pei; Dai, Shengjie; Duan, Chaorui; Wu, Yan; Leng, Ping

    2012-01-01

    Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (Solanum lycopersicum) fruit ripening, we suppressed SlNCED1, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress SlNCED1 specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (SlPG), pectin methyl esterase (SlPME), β-galactosidase precursor mRNA (SlTBG), xyloglucan endotransglycosylase (SlXET), endo-1,4-β-cellulose (SlCels), and expansin (SlExp). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (SlPG, SlPME, SlTBG, SlXET, SlCels, and SlExp). PMID:22108525

  4. ExCEL in Social Work: Excellence in Cancer Education & Leadership: An Oncology Social Work Response to the 2008 Institute of Medicine Report.

    Science.gov (United States)

    Otis-Green, Shirley; Jones, Barbara; Zebrack, Brad; Kilburn, Lisa; Altilio, Terry A; Ferrell, Betty

    2015-09-01

    ExCEL in Social Work: Excellence in Cancer Education & Leadership was a multi-year National Cancer Institute (NCI)-funded grant for the development and implementation of an innovative educational program for oncology social workers. The program's curriculum focused upon six core competencies of psychosocial-spiritual support necessary to meet the standard of care recommended by the 2008 Institute of Medicine (IOM) Report: Cancer Care for the Whole Patient: Meeting Psychosocial Health Needs. The curriculum was delivered through a collaborative partnership between the City of Hope National Medical Center and the two leading professional organizations devoted exclusively to representing oncology social workers--the Association of Oncology Social Work and the Association of Pediatric Oncology Social Workers. Initial findings support the feasibility and acceptability of this tailored leadership skills-building program for participating oncology social workers.

  5. Implications of the Mw9.0 Tohoku-Oki earthquake for ground motion scaling with source, path, and site parameters

    Science.gov (United States)

    Stewart, Jonathan P.; Midorikawa, Saburoh; Graves, Robert W.; Khodaverdi, Khatareh; Kishida, Tadahiro; Miura, Hiroyuki; Bozorgnia, Yousef; Campbell, Kenneth W.

    2013-01-01

    The Mw9.0 Tohoku-oki Japan earthquake produced approximately 2,000 ground motion recordings. We consider 1,238 three-component accelerograms corrected with component-specific low-cut filters. The recordings have rupture distances between 44 km and 1,000 km, time-averaged shear wave velocities of VS30 = 90 m/s to 1,900 m/s, and usable response spectral periods of 0.01 sec to >10 sec. The data support the notion that the increase of ground motions with magnitude saturates at large magnitudes. High-frequency ground motions demonstrate faster attenuation with distance in backarc than in forearc regions, which is only captured by one of the four considered ground motion prediction equations for subduction earthquakes. Recordings within 100 km of the fault are used to estimate event terms, which are generally positive (indicating model underprediction) at short periods and zero or negative (overprediction) at long periods. We find site amplification to scale minimally with VS30 at high frequencies, in contrast with other active tectonic regions, but to scale strongly with VS30 at low frequencies.

  6. Elaboração e caracterização de cookies sem glúten enriquecidos com farinha de coco: uma alternativa para celíacos

    Directory of Open Access Journals (Sweden)

    Ana Maria Queiroz

    2017-05-01

    Full Text Available Resumo Os celíacos encontram dificuldades na adoção de uma dieta totalmente isenta de glúten e com qualidade nutricional. Para tanto, objetivou-se desenvolver e avaliar cookies sem glúten enriquecidos com farinha de coco. A farinha de coco com elevado teor de cinzas, proteínas e lipídios pode ser utilizada para enriquecer produtos alimentícios. Foram elaborados cookies com base na composição de uma mistura da Federação Nacional das Associações de Celíacos do Brasil - Fenacelbra (“mix de farinha preparada II” para cookies sem glúten: Padrão FP (formulação semelhante à composição do “mix de farinha preparada II” da Fenacelbra, F1 (modificação de FP com substituição da fécula de batata por 10% de farinha de coco, F2 (modificação de FP com adição de 5% de farinha de coco, F3 (modificação de FP com substituição do polvilho doce por 5% de farinha de coco. Os cookies foram avaliados quanto às características físicas (peso pós-cocção, diâmetro e espessura pós-cocção, fator de expansão, rendimento, Aw, dureza e cor – L*a*b*, físico-químicas (umidade, cinzas, proteínas, lipídios, carboidratos e valor calórico, aceitabilidade quanto aos atributos sensoriais (aceitação global, aparência, cor, aroma, sabor e textura e atitude de compra. A adição de farinha de coco às formulações melhorou as propriedades nutricionais dos cookies sem glúten, aumentando o teor de lipídios e proteínas, e reduzindo o teor de carboidratos. Os cookies desenvolvidos apresentaram boa aceitação sensorial e intenção de compra satisfatória., A adição de 10% de farinha de coco em substituição da fécula de batata na formulação F1 não alterou as características sensoriais do produto, porém proporcionou propriedades físicas e físico-químicas superiores. Dessa forma, cookies sem glúten enriquecidos com farinha de coco constituem uma alternativa viável de alimentos destinados para pessoas portadoras de

  7. Performance of JMA Earthquake Early Warning for the 2011 off the Pacific coast of Tohoku Earthquake (Mw9.0)

    Science.gov (United States)

    Hoshiba, M.; Wakayama, A.; Ishigaki, Y.; Doi, K.

    2011-12-01

    This presentation outlines the Earthquake Early Warning of the Japan Meteorological Agency (JMA) for the 2011 off the Pacific coast of Tohoku Earthquake (Mw9.0). EEW has been operational nationwide in Japan by JMA since October, 2007. For JMA EEW, the hypocenter is determined by a combination of several techniques, using approximately 1,100 stations from the JMA network and the Hi-net network of NIED; magnitude is mainly from maximum displacement amplitudes. JMA EEWs are updated as available data increases with elapsed time. Accordingly EEWs are issued repeatedly with improving accuracy for a single earthquake. JMA EEWs are divided into two grades depending on the expected intensities. The JMA intensity scale is based on instrumental measurements in which not only the amplitude but also the frequency and duration of the shaking are considered. The 10-degree JMA intensity scale rounds off the instrumental intensity value to the integer. Intensities of 5 and 6 are divided into two degrees, namely 5-lower, 5-upper, 6-lower and 6-upper, respectively. Intensity 1 corresponds to ground motion that people can barely detect, and 7 is the upper limit. JMA EEWs are announced to general public when intensity 5-lower (or greater) is expected. The JMA EEW system was triggered for the Mw 9.0 earthquake when station OURI (138km from the epicenter) detected the initial P wave at 14:46:40.2 (Japan Standard Time). The first EEW, the first of 15 announcements, was issued 5.4 s later. The waveform started with small amplitude, which was comparable to noise level for displacement. The small amplitude does not indicate that the initial rupture of the Mw 9.0 event is large, and does not suggest a large magnitude event. By the fourth EEW, 8.6 s after the first trigger, the expected intensity exceeded the criteria of the warning to the general public. JMA issued the fourth EEW announcements to the general public of the Tohoku district, and then the warning was automatically broadcast

  8. Country report: Syria. Development of {sup 90}Y/{sup 90}Sr Generator and {sup 90}Y Radiopharmaceuticals

    Energy Technology Data Exchange (ETDEWEB)

    Yassine, Taufik; Mukhallalati, Ch. Heyam, E-mail: atomic@aec.org.sy [Atomic Energy Commission of Syria, Damascus (Syrian Arab Republic)

    2010-07-01

    The aim of this project is to develop a technique for preparation of {sup 90}Sr-{sup 90}Y generator, we have developed a separation technique for isolation of {sup 90}Y from {sup 90}Sr based on using Sr – Spec resin packed in three columns for separation and purification of {sup 90}Sr-{sup 90}Y .The resulting Y90 is used for therapeutic applications. The first part of this project describe a prototype design for the {sup 90}Sr- {sup 90}Y generator in order to get a very accurate method to obtain the minimum possible {sup 90}Sr Breakthrough. 25 mci of {sup 90}Sr was used in the generator {sup 90}Sr-{sup 90}Y and we obtained the elution yield of {sup 90}Y higher than 88%, Also the eluate was used for preparation of several {sup 90}Y radiopharmaceuticals such as {sup 90}YEDTMP and {sup 90}Y-DOTA-HR{sub 3}. And the work is continues to investigate more radiopharmaceuticals applications in the second part of this project such as {sup 90}Y– FHMA. In this part of the co-coordination research programmer, A protocol based on results of this studies was developed to prepare and operate a higher activity generator (50-100mci), the resulting elution yield was approximately 94% of {sup 90}Y . The elute was used in preparation of new {sup 90}Y radiopharmaceuticals. The monoclonal antibodies is still evolving by conjugate Rituximab to The macrocyclic bifunctional chelating agent,(p-SCN-Bn-DOTA)S-2-(4-Isothiocyanatobenzyle)-1,4,7,10- tetraazacyclododecane-tetraaceticacid to obtain the inmunoconjugate DOTA-Rituximub in simple way and then investigating the radio labeling conditions with {sup 90}Y. (author)

  9. New engineering treatment of bovine pericardium confers outstanding resistance to calcification in mitral and pulmonary implantations in a juvenile sheep model.

    Science.gov (United States)

    Brizard, Christian P; Brink, Johann; Horton, Steven B; Edwards, Glenn Anthony; Galati, John C; Neethling, William M L

    2014-12-01

    To conduct a test of noninferiority for CardioCel (Admedus, Brisbane, Australia), a chemically engineered bovine pericardium over autologous pericardium treated intraoperatively with glutaraldehyde in a chronic juvenile sheep model of pulmonary valve (PV) and mitral valve (MV) reconstruction. We replaced the posterior leaflet of the MV and of 1 PV cusp with patches in ewes aged 10 months. There were 2 groups: CardioCel (n = 6) and control (n = 4). All valves were competent. Echocardiography was performed before euthanasia. The collected data were function, macroscopy, histology, and calcium contents. The primary end points were thickening and calcium content. All animals survived until sacrifice after 7 months. The valves had normal echo. The macroscopic aspect of the valves was excellent. Examination of the slides for both groups revealed a continuous endothelium on both sides of the patch and a layer of new collagen developed on both sides between patch and endothelium and interstitial cells and smooth muscle cell in these layers. The patch had not thickened but the 2 layers of new collagen for the PV showed a median thickening of 37% in the CardioCel group and 111% in the control group (P = .01), and for the MV a thickening of 108% and 251%, respectively, was seen (P = .01). The median calcium content in the PV was 0.24 μg/mg (range, 0.19-0.30) in the CardioCel group versus 0.34 μg/mg (range, 0.24-0.62) in controls (P = .20). In the MV it was 0.46 μg/mg (range, 0.30-1.0) in the CardioCel group and 0.47 μg/mg (range, 0.29-1.9) in controls (P = 1.0). In this growing lamb model the CardioCel patch allowed accurate valve repair at both systemic and pulmonary pressure. The mechanical properties of CardioCel after 7 months were preserved with a more controlled healing than the treated autologous pericardium and without calcification. Copyright © 2014 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

  10. Gamma ray production cross sections for the interactions of 14.9 MeV neutrons with C, Al, V, Fe and Nb at 90 deg

    International Nuclear Information System (INIS)

    Zhou Hongyu; Tang Lin; Yan Yiming

    1986-11-01

    Discrete γ-ray spectra and production cross sections for the interactions of 14.9 MeV neutrons with C, Al, V, Fe and Nb targets have been measured at 90 deg. using a 110.7cm 3 Ge(Li) detector. A single γ-ray peak was measured for C. For Al 34 γ lines, for Fe 41 γ lines, for V 61 γ lines and for Nb 79 γ lines were measured

  11. Overexpression of GmHsp90s, a heat shock protein 90 (Hsp90 gene family cloning from soybean, decrease damage of abiotic stresses in Arabidopsis thaliana.

    Directory of Open Access Journals (Sweden)

    Jinyan Xu

    Full Text Available Hsp90 is one of the most conserved and abundant molecular chaperones and is an essential component of the protective stress response; however, its roles in abiotic stress responses in soybean (Glycine max remain obscure. Here, 12 GmHsp90 genes from soybean were identified and found to be expressed and to function differentially under abiotic stresses. The 12 GmHsp90 genes were isolated and named GmHsp90A1-GmHsp90A6, GmHsp90B1, GmHsp90B2, GmHsp90C1.1, GmHsp90C1.2, GmHsp90C2.1 and GmHsp90C2.2 based on their characteristics and high homology to other Hsp90s according to a new nomenclature system. Quantitative real-time PCR expression data revealed that all the genes exhibited higher transcript levels in leaves and could be strongly induced under heat, osmotic and salt stress but not cold stress. Overexpression of five typical genes (GmHsp90A2, GmHsp90A4, GmHsp90B1, GmHsp90C1.1 and GmHsp90C2.1 in Arabidopsis thaliana provided useful evidences that GmHsp90 genes can decrease damage of abiotic stresses. In addition, an abnormal accumulation of proline was detected in some transgenic Arabidopsis plants suggested overexpressing GmHsp90s may affect the synthesis and response system of proline. Our work represents a systematic determination of soybean genes encoding Hsp90s, and provides useful evidence that GmHsp90 genes function differently in response to abiotic stresses and may affect the synthesis and response system of proline.

  12. Separation of 90Y from 90Sr by solvent extraction with ionizable crown ethers

    International Nuclear Information System (INIS)

    Wood, D.J.; Elshani, S.; Du, H.S.; Natale, N.R.; Wai, C.M.

    1993-01-01

    Sym-Dibenzo-16-crown-5-oxyacetic acid and its analogues are selective for the extraction of Y 3+ over Sr 2+ from aqueous solutions into chloroform. The selectivity and the pH range of extraction are influenced by the structure of the ligand. The size of the macrocyclic cavity, the tether length of the lariat, the attachment of alkyl functional groups to the lariat, and the identity of the ionizable group can affect selectivity and extraction efficiency. When the carboxylic acid at the terminal end of the lariat is replaced by a hydroxamic acid, the selectivity for Y 3+ over Sr 2+ is significantly increased. Using these ionizable crown ethers as extractants, 90 Y fractions of greater than 99.9% purity can be obtained in a single solvent extraction step from solutions of 90 Sr. 11 refs., 5 figs., 2 tabs

  13. ExCEL in Social Work: Excellence in Cancer Education & Leadership An Oncology Social Work Response to the 2008 Institute of Medicine Report

    Science.gov (United States)

    Otis-Green, Shirley; Jones, Barbara; Zebrack, Brad; Kilburn, Lisa; Altilio, Terry A.; Ferrell, Betty

    2014-01-01

    ExCEL in Social Work : Excellence in Cancer Education & Leadership was a multi-year National Cancer Institute (NCI)-funded grant for the development and implementation of an innovative educational program for oncology social workers. The program’s curriculum focused upon six core competencies of psychosocial-spiritual support necessary to meet the standard of care recommended by the 2008 Institute of Medicine (IOM) Report: Cancer Care for the Whole Patient: Meeting Psychosocial Health Needs. The curriculum was delivered through a collaborative partnership between the City of Hope National Medical Center and the two leading professional organizations devoted exclusively to representing oncology social workers - the Association of Oncology Social Work and the Association of Pediatric Oncology Social Workers. Initial findings support the feasibility and acceptability of this tailored leadership skills-building program for participating oncology social workers. PMID:25146345

  14. Targeting Hsp90-Cdc37: A Promising Therapeutic Strategy by Inhibiting Hsp90 Chaperone Function.

    Science.gov (United States)

    Wang, Lei; Li, Li; Gu, Kai; Xu, Xiao-Li; Sun, Yuan; You, Qi-Dong

    2017-01-01

    The Hsp90 chaperone protein regulates the folding, maturation and stability of a wide variety of oncoproteins. In recent years, many Hsp90 inhibitors have entered into the clinical trials while all of them target ATPase showing similar binding capacity and kinds of side-effects so that none have reached to the market. During the regulation progress, numerous protein- protein interactions (PPI) such as Hsp90 and client proteins or cochaperones are involved. With the Hsp90-cochaperones PPI networks being more and more clear, many cancerous proteins have been reported to be tightly correlated to Hsp90-cochaperones PPI. Among them, Hsp90-Cdc37 PPI has been widely reported to associate with numerous protein kinases, making it a novel target for the treatment of cancers. In this paper, we briefly review the strategies and modulators targeting Hsp90-Cdc37 complex including direct and indirect regulation mechanism. Through these discussions we expect to present inspirations for new insights into an alternative way to inhibit Hsp90 chaperone function. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  15. Use of a 90° drill and screwdriver for rib fracture stabilization.

    Science.gov (United States)

    Nickerson, Terry P; Kim, Brian D; Zielinski, Martin D; Jenkins, Donald; Schiller, Henry J

    2015-03-01

    Rib fracture stabilization has become a more accepted practice although stabilization of the most cephalad ribs presents a unique challenge. We present our experience with use of a 90° drill and screwdriver to bridge these difficult rib fractures. This retrospective review included patients who underwent rib fracture stabilization from August 1, 2009, through September 30, 2012. Patients were divided into two groups: those whose procedure used the 90° device and those that did not. Data were compared using standard statistical analysis and reported as percentages and medians [interquartile ranges]. P values rib stabilized was different between the 2 groups (3 [2-5] vs. 5 [2-9]; P = 0.001), with more third rib stabilizations in the 90° group (38 vs. 20%; P = 0.04) as well as more total number of ribs fixed (5 vs. 4; P = 0.001). There was no difference in operative time between the 2 groups. The surgical reach for rib fracture stabilization has been extended with use of a 90° drill and screwdriver. High fractures under the scapula where access is technically challenging can be stabilized without prolonging operative times.

  16. Development of a dosimetric system for 90Sr + 90Y betatherapy applicators

    International Nuclear Information System (INIS)

    Coelho, Talita Salles

    2010-01-01

    The 90 Sr+ 90 Y applicators, used in betatherapy for prevention of keloids and pterigium, are imported and many times their dosimetric features are shown only in an illustrated form by the manufacturers. The exhaustive routine of the medical physicists in the clinic do not make possible the accomplishment of procedures for the confirmation of these parameters. This work presents the development of a methodology for the dosimetry of 90 Sr+ 90 Y betatherapy applicators. The Monte Carlo code MCNP5 was used for the simulation of the percentage depth dose curves and dose distribution profiles produced by these applicators. The experimental measurements of the radial and axial radiation attenuation, have been done with a mini-extrapolation chamber, thermoluminescent dosimeters and radiographic films. The experimental results have been compared with the simulated values. Both percentage depth dose curves and the radial dose profiles, the theoretical and the experimental ones, have presented good agreement, which may validate the use of the MCNP5 for these simulations, confirming the viability of the usage of this method in procedures of beta emitter sources dosimetry. (author)

  17. Gardasil-9: A global survey of projected efficacy.

    Science.gov (United States)

    Zhai, Lukai; Tumban, Ebenezer

    2016-06-01

    Human papillomaviruses (HPVs) are the causative agents of human neoplasias such as warts and cancers. There are ∼19 HPV types associated with cancers, which has made it very challenging for first generation HPV vaccines to offer complete protection against all cancer-causing HPV types. Recently, a second generation HPV vaccine, Gardasil-9, has been approved to protect against more HPV types. Worldwide, Gardasil-9 will protect against HPV types associated with ∼90% of cervical cancer case in women and 80-95% of other HPV-associated anogenital cancers in both men and women. However, due to variation in HPV-type specific prevalence and distribution, the vaccine will offer different percentages of protection in different geographical regions; Gardasil-9 will offer protection against HPV types associated with ∼87.7% of cervical cancers in Asia, 91.7% in Africa, 92% in North America, 90.9% in Europe, 89.5% in Latin America & the Caribbean, and 86.5% in Australia. Because of this, Pap smear screening and testing for HPV types not included in Gardasil-9 will need to continue, especially in HIV/AIDS patients. In order to achieve complete protection against all HPV types that cause cervical cancer, a third-generation HPV vaccine is needed. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Development of a bifunctional xylanase-cellulase chimera with enhanced activity on rice and barley straws using a modular xylanase and an endoglucanase procured from camel rumen metagenome.

    Science.gov (United States)

    Khalili Ghadikolaei, Kamran; Akbari Noghabi, Kambiz; Shahbani Zahiri, Hossein

    2017-09-01

    The camel rumen metagenome is an untapped source of glycoside hydrolases. In this study, novel genes encoding for a modular xylanase (XylC) and a cellulase (CelC) were isolated from a camel rumen metagenome and expressed in Escherichia coli BL21 (DE3). XylC with xylanase (Xyn), CBM, and carbohydrate esterase (CE) domains was characterized as a β-1,4-endoxylanase with remarkable catalytic activity on oat-spelt xylan (K cat  = 2919 ± 57 s -1 ). The implication of XylC's modular structure in its high catalytic activity was analyzed by truncation and fusion construction with CelC. The resulting fusions including Cel-CBM, Cel-CBM-CE, and Xyn-CBM-Cel showed remarkable enhancement in CMCase activity with K cat values of 742 ± 12, 1289 ± 34.5, and 2799 ± 51 s -1 compared to CelC with a K cat of 422 ± 3.5 s -1 . It was also shown that the bifunctional Xyn-CBM-Cel with synergistic xylanase/cellulase activities was more efficient than XylC and CelC in hydrolysis of rice and barley straws.

  19. Protocolul persan de la Cirus cel Mare până la Chosroes I

    Directory of Open Access Journals (Sweden)

    Orest TĂRÎȚĂ

    2017-12-01

    Full Text Available În articol se abordează unele aspecte - cheie ale protocolului și ceremonialului persan din tim-pul celor patru perioade istorice ale statalității persane începând cu anul 700 până la Hristos și finalizând cu anul 651 ale erei creștine. Prin prisma analizei este trecută domnia lui Cirus al II-lea cel Mare – fondatorul Imperiului Per-san, care a introdus la curtea sa protocolul și ceremonialul pentru a-i debarasa pe persani de obiceiurile barbare și a-i familiariza cu subtilitățile bunelor maniere. Un spațiu aparte este rezervat perioadei sasanide (224 î. Hr. - 651, când curtea regală este con¬dusă de șeful de protocol, situat pe primul loc la curtea șahinșahului, fiind urmat de succesorul la tron, șeful regimentului de „nemuritori” și alte demnități; este descrisă scena încoronării lui Șapur I și ordinea ierarhică a înalților demnitari regali de la curtea sa, primirea cu onoruri a ambasa¬dorilor străini la curte și alaiurile publice ale șahinșahului, care urmăreau nu altceva decât să perpetueze măreția Marelui Imperiu, atât în interior, cât și în relațiile cu țările vecine.

  20. Development of an electrochemical 90Sr-90Y generator for separation of 90Y suitable for targeted therapy

    International Nuclear Information System (INIS)

    Chakravarty, Rubel; Pandey, Usha; Manolkar, Remani B.; Dash, Ashutosh; Venkatesh, Meera; Pillai, M.R. Ambikalmajan

    2008-01-01

    90 Y of high specific activity and very high radionuclidic purity (>99.998%) is essential for targeted therapy. Since the current methods used for the preparation of 90 Y from 90 Sr are not adaptable for use in a central/hospital radiopharmacy, a simple 90 Sr- 90 Y generator system based on electrochemical separation technique was developed. Methods: Two-cycle electrolysis procedure was developed for separation of 90 Y from 90 Sr in nitrate solution. The first electrolysis was performed for 90 min in 90 Sr(NO 3 ) 2 feed solution at pH 2-3 at a potential of -2.5V with 100-200 mA current using platinum electrodes. The second electrolysis was performed for 45 min in 3 mM HNO 3 at a potential of -2.5V with 100 mA current. In this step, the cathode from the first electrolysis containing 90 Y was used as anode along with a fresh circular platinum electrode as cathode. The 90 Y deposited on the circular cathode after the second electrolysis was dissolved in acetate buffer to obtain 90 Y acetate, suitable for radiolabeling. Results: >96% recovery of 90 Y could be achieved in each cycle, with an overall decay corrected yield of >90%. The recovered 90 Y had high radionuclidic purity with barely 30.2±15.2 kBq (817±411 nCi) of 90 Sr per 37 GBq (1 Ci) of 90 Y (0.817±0.411 ppm). Consistent and repeated separation could be demonstrated using up to 1.85 GBq (50 mCi) of 90 Sr. The generator was named 'Kamadhenu,' the eternally milk-yielding Indian mythological cow. Conclusions: A technique suitable for adaptation at central radiopharmacies for obtaining therapeutic quantities of pure 90 Y has been developed

  1. Spatio-temporal regulation of Hsp90-ligand complex leads to immune activation.

    Directory of Open Access Journals (Sweden)

    Yasuaki eTamura

    2016-05-01

    Full Text Available Hsp90 is the most abundant cytosolic HSP and is known to act as a molecular chaperone. We found that an Hsp90-cancer antigen peptide complex was efficiently cross-presented by human monocyte-derived dendritic cells and induced peptide-specific cytotoxic T lymphocytes. Furthermore, we observed that the internalized Hsp90-peptide complex was strictly sorted to the Rab5+, EEA1+ static early endosome and the Hsp90-chaperoned peptide was processed and bound to MHC class I molecules through a endosome-recycling pathway. We also found that extracellular Hsp90 complexed with CpG-A or self-DNA stimulates production of a large amount of IFN-α from pDCs via static early endosome targeting. Thus, extracellular Hsp90 can target the antigen or nucleic acid to a static early endosome by spatio-temporal regulation. Moreover, we showed that Hsp90 associates with and delivers TLR7/9 from the ER to early endosomes for ligand recognition. Hsp90 inhibitor, geldanamycin derivative inhibited the Hsp90 association with TLR7/9, resulting in inhibition IFN-α production, leading to improvement of SLE symptoms. Interstingly, we observed that serum Hsp90 is clearly increased in patients with active SLE compared with that in patients with inactive disease. Serum Hsp90 detected in SLE patients binds to self-DNA and/or anti-DNA Ab, thus leading to stimulation of pDCs to produce IFN-α. Thus, Hsp90 plays a crucial role in the pathogenesis of SLE and that an Hsp90 inhibitor will therefore provide a new therapeutic approach to SLE and other nucleic acid-related autoimmune diseases. We will discuss how spatio-temporal regulation of Hsp90-ligand complexes within antigen-presenting cells affects the innate immunity and adaptive immunity.

  2. Listening to data from the 2011 magnitude 9.0 Tohoku-Oki, Japan, earthquake

    Science.gov (United States)

    Peng, Z.; Aiken, C.; Kilb, D. L.; Shelly, D. R.; Enescu, B.

    2011-12-01

    It is important for seismologists to effectively convey information about catastrophic earthquakes, such as the magnitude 9.0 earthquake in Tohoku-Oki, Japan, to general audience who may not necessarily be well-versed in the language of earthquake seismology. Given recent technological advances, previous approaches of using "snapshot" static images to represent earthquake data is now becoming obsolete, and the favored venue to explain complex wave propagation inside the solid earth and interactions among earthquakes is now visualizations that include auditory information. Here, we convert seismic data into visualizations that include sounds, the latter being a term known as 'audification', or continuous 'sonification'. By combining seismic auditory and visual information, static "snapshots" of earthquake data come to life, allowing pitch and amplitude changes to be heard in sync with viewed frequency changes in the seismograms and associated spectragrams. In addition, these visual and auditory media allow the viewer to relate earthquake generated seismic signals to familiar sounds such as thunder, popcorn popping, rattlesnakes, firecrackers, etc. We present a free software package that uses simple MATLAB tools and Apple Inc's QuickTime Pro to automatically convert seismic data into auditory movies. We focus on examples of seismic data from the 2011 Tohoku-Oki earthquake. These examples range from near-field strong motion recordings that demonstrate the complex source process of the mainshock and early aftershocks, to far-field broadband recordings that capture remotely triggered deep tremor and shallow earthquakes. We envision audification of seismic data, which is geared toward a broad range of audiences, will be increasingly used to convey information about notable earthquakes and research frontiers in earthquake seismology (tremor, dynamic triggering, etc). Our overarching goal is that sharing our new visualization tool will foster an interest in seismology, not

  3. Yttrium-90

    International Nuclear Information System (INIS)

    Ammerich, Marc; Frot, Patricia; Gambini, Denis-Jean; Gauron, Christine; Moureaux, Patrick; Herbelet, Gilbert; Lahaye, Thierry; Pihet, Pascal; Rannou, Alain; Vial, Eric

    2013-03-01

    This sheet belongs to a collection which relates to the use of radionuclides essentially in unsealed sources. Its goal is to gather on a single document the most relevant information as well as the best prevention practices to be implemented. These sheets are made for the persons in charge of radiation protection: users, radioprotection-skill persons, labor physicians. Each sheet treats of: 1 - the radio-physical and biological properties; 2 - the main uses; 3 - the dosimetric parameters; 4 - the measurement; 5 - the protection means; 6 - the areas delimitation and monitoring; 7 - the personnel classification, training and monitoring; 8 - the effluents and wastes; 9 - the authorization and declaration administrative procedures; 10 - the transport; and 11 - the right conduct to adopt in case of incident or accident. This sheet deals specifically with Yttrium-90

  4. Dynamic study of a compressed electron layer during the hole-boring stage in a sharp-front laser interaction region

    Directory of Open Access Journals (Sweden)

    W. P. Wang

    2012-08-01

    Full Text Available This study investigates the dynamics of a compressed electron layer (CEL when a circularly polarized laser pulse with a sharp front irradiates a high-density foil. A time-dependent model for CEL motion during the hole-boring stage is proposed to describe details of the interaction for any shape of laser pulse. The opacity case, where the laser pulse is totally reflected, is investigated using this model. The results obtained are consistent with the results from particle-in-cell (PIC simulations. A relaxation distance determined by the laser-front steepness is necessary to build a stable CEL state before ions rejoin into the CEL. For the transparent case, the laser-front steepness is important for the formation of the stable CEL state at the back surface of the target. Considering the motion of ions, both the CEL and ion dynamics are important to rebalance the laser pressure and electrostatic charge-separation force as the hole-boring stage changes to the light-sail stage.

  5. Development of GE90 engine with largest thrust. GE90 engine no kaihatsu jokyo

    Energy Technology Data Exchange (ETDEWEB)

    Aono, H [Ishikawajima-Harima Heavy Industries, Co. Ltd., Tokyo (Japan)

    1994-05-01

    The present paper explained the turbofan engine GE90 which is being developed by General Electric Co., USA. That engine is to meet the thrust (takeoff thrust) of 300 to 530kN as required for the new-generation wide-fuselage civil transport plane which is being designed for its planned operation in the 1990's. In April, 1991, the world's strongest thrust of 480kN was achieved with engine elements also confirmed through element test. Thereafter, the engine underwent a flying test on board of Boeing 747 to materialize the planned operation in 1995. Made to be 9 in by-pass ratio and about 40 in overall pressure ratio, the GE90 was given the concept that advantage could be secured in both propulsive efficiency and thermal efficiency. That concept could be materialized by the development of composite fan blade technology and energy-efficient technology which were both demonstrated with an unducted fan. In spite of its pressure ratio of 22, the GE90's high pressure compressor demonstrates its polytropic efficiency which is equal to that of the low pressure ratio compressor. 3 refs., 19 figs., 1 tab.

  6. 49 CFR 1242.86 - Industrial development (account XX-61-90).

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Industrial development (account XX-61-90). 1242.86 Section 1242.86 Transportation Other Regulations Relating to Transportation (Continued) SURFACE....86 Industrial development (account XX-61-90). These accounts pertain solely to freight service and...

  7. Treatment of advanced pancreatic carcinoma with 90Y-Clivatuzumab Tetraxetan: a phase I single-dose escalation trial.

    Science.gov (United States)

    Gulec, Seza A; Cohen, Steven J; Pennington, Kenneth L; Zuckier, Lionel S; Hauke, Ralph J; Horne, Heather; Wegener, William A; Teoh, Nick; Gold, David V; Sharkey, Robert M; Goldenberg, David M

    2011-06-15

    Humanized antibody hPAM4 specifically binds a mucin glycoprotein expressed in pancreatic adenocarcinomas. This phase I study evaluated a single dose of (90)Y-clivatuzumab tetraxetan ((90)Y-labeled hPAM4) in patients with advanced pancreatic cancer. Twenty-one patients (4 stage III; 17 stage IV) received (111)In-hPAM4 for imaging and serum sampling before (90)Y-hPAM4. Study procedures evaluated adverse events, safety laboratories, computed tomography (CT) scans, biomarkers, pharmacokinetics, radiation dosimetry, and immunogenicity (HAHA). (111)In-hPAM4 showed normal biodistribution with radiation dose estimates to red marrow and solid organs acceptable for radioimmunotherapy and with tumor targeting in 12 patients. One patient withdrew before (90)Y-hPAM4; otherwise, 20 patients received (90)Y doses of 15 (n = 7), 20 (n = 9), and 25 mCi/m(2) (n = 4). Treatment was well tolerated; the only significant drug-related toxicities were (NCI CTC v.3) grade 3 to 4 neutropenia and thrombocytopenia increasing with (90)Y dose. There were no bleeding events or serious infections, and most cytopenias recovered to grade 1 within 12 weeks. Three patients at 25 mCi/m(2) encountered dose-limiting toxicity with grade 4 cytopenias more than 7 days, establishing 20 mCi/m(2) as the maximal tolerated (90)Y dose. Two patients developed HAHA of uncertain clinical significance. Most patients progressed rapidly and with CA19-9 levels increasing within 1 month of therapy, but 7 remained progression-free by CT for 1.5 to 5.6 months, including 3 achieving transient partial responses (32%-52% tumor diameter shrinkage). (90)Y-Clivatuzumab tetraxetan was well tolerated with manageable hematologic toxicity at the maximal tolerated (90)Y dose, and is a potential new therapeutic for advanced pancreatic cancer. ©2011 AACR.

  8. PREDICE score as a predictor of 90 days mortality in patients with heart failure

    Science.gov (United States)

    Purba, D. P. S.; Hasan, R.

    2018-03-01

    Hospitalization in chronic heart failure patients associated with high mortality and morbidity rate. The 90 days post-discharge period following hospitalization in heart failure patients is known as the vulnerable phase, it carries the high risk of poor outcomes. Identification of high-risk individuals by using prognostic evaluation was intended to do a closer follow up and more intensive to decreasing the morbidity and mortality rate of heart failure.To determine whether PREDICE score could predict mortality within 90 days in patients with heart failure, an observational cohort study in patients with heart failure who were hospitalized due to worsening chronic heart failure. Patients were in following-up for up to 90 days after initial evaluation with the primary endpoint is death.We found a difference of the significantstatistical between PREDICE score in survival and mortality group (p=0.001) of 84% (95% CI: 60.9% - 97.4%).In conclusion, PREDICE score has a good ability to predict mortality within 90 days in patients with heart failure.

  9. Effects of radiolysis on yttrium-90-labeled Lym-1 antibody preparations.

    Science.gov (United States)

    Salako, Q A; O'Donnell, R T; DeNardo, S J

    1998-04-01

    The physical half-life of 2.6 days and 2.2 MeV beta emissions of 90Y provide excellent properties for radioimmunotherapy applications. However, the clinically useful beta particles may be a source of radiation-induced damage of 90Y-labeled immunoconjugate radiopharmaceuticals during preparation or short-term storage. The stability of 90Y-labeled Lym-1 antibody was studied in standard radiopharmacy conditions to establish a formulation at which radiolysis is not a problem. Lym-1-21T-BAD immunoconjugate intermediate was prepared according to our standard procedure, then labeled with 90Y at 1, 2, 4 and 9.4 mCi/mg Lym-1 using 0.5 M tetramethylammonium acetate, pH 7, labeling buffer. Each mixture was challenged in diethylenetriaminepentaacetic acid to remove nonspecifically bound 90Y. The 90Y-21T-BAD-Lym-1 products were purified by centrifuged molecular sieving column chromatography. The radiochemical purity and immunoreactivity of each preparation was monitored daily by high-performance liquid chromatography (HPLC) and solid-phase radioimmunoassay, respectively, for 3 days. The preparation at 2 mCi/mg was also formulated in 4% (wt/vol) human serum albumin (HSA) overall and at 9.4 mCi/mg in five-fold water, 4 and 10% (wt/vol) HSA overall; all were monitored as above. The monomeric quality and purity profile of products at 1 and 2 mCi/mg were retained (> or = 80%) as was their immunoreactivity (> or = 75%) over 3 days. The radiochemical purity and immunoreactivity of the product at 4 mCi/mg declined to 65% and 28%, respectively, by 3 days after preparation and in just 48 hr, the product at 9.4 mCi/mg had degraded to 21% in radiochemical purity with only 3% immunoreactivity. The current HPLC data and earlier published chromatographic evidence did not support a compromised radiochemical integrity of 90Y-DOTA complexes by loss of 90Y from the DOTA chelate. Radiolysis of 90Y-labeled antibody preparations did not appear to be a problem at 90Y-21T-BAD-Lym-1 products < or = 2 m

  10. In vitro and in vivo evaluation of the indoloquinone EO-9 (NSC 382 459) against human small cell carcinoma of the lung

    DEFF Research Database (Denmark)

    Roed, H; Aabo, K; Vindeløv, L

    1989-01-01

    As the indoloquinone EO-9 has previously shown activity in several tumor model systems it was evaluated against four human small cell lung cancer cell lines by the clonogenic assay. In two cell lines (Nyh and Tol), exponential dose-response curves were achieved with both 1 h and continuous exposure......, whereas no cell kill was obtained in the other two cell lines (69 and 592) when tested with 1 h incubation up to 0.25 microgram/ml. When the cells were exposed to drug in vitro, flow cytometric DNA analysis showed perturbations in the cell cycle distribution of the most sensitive cell line (Tol......) at a lower EO-9 concentration than in the less sensitive cel line (592). This in vitro predicted difference in EO-9 sensitivity between two of the cell lines (592 and Tol) was confirmed when the cell lines were heterotransplanted to nude mice....

  11. Rapid Source Characterization of the 2011 Mw 9.0 off the Pacific coast of Tohoku Earthquake

    Science.gov (United States)

    Hayes, Gavin P.

    2011-01-01

    On March 11th, 2011, a moment magnitude 9.0 earthquake struck off the coast of northeast Honshu, Japan, generating what may well turn out to be the most costly natural disaster ever. In the hours following the event, the U.S. Geological Survey National Earthquake Information Center led a rapid response to characterize the earthquake in terms of its location, size, faulting source, shaking and slip distributions, and population exposure, in order to place the disaster in a framework necessary for timely humanitarian response. As part of this effort, fast finite-fault inversions using globally distributed body- and surface-wave data were used to estimate the slip distribution of the earthquake rupture. Models generated within 7 hours of the earthquake origin time indicated that the event ruptured a fault up to 300 km long, roughly centered on the earthquake hypocenter, and involved peak slips of 20 m or more. Updates since this preliminary solution improve the details of this inversion solution and thus our understanding of the rupture process. However, significant observations such as the up-dip nature of rupture propagation and the along-strike length of faulting did not significantly change, demonstrating the usefulness of rapid source characterization for understanding the first order characteristics of major earthquakes.

  12. Absolute Standardization Of 90Sr Using 4 pi(PC) Detector

    International Nuclear Information System (INIS)

    Pujadi; Wardiyanto, Gatot; Wijaya I, Nazar; Sudarsono

    2000-01-01

    Standardization of exp.90 Sr with absolute measurement using 4 pi proporsional detector has been carried out. The counting efficiency of beta particle mainly depends on self and VYNS absorption. The correction of self absorption were determined by means of the beta maximum energy vs. absorption curve. Self absorption value of exp.90 Sr with Emax. 0,55 MeV is 2,5%, and exp.90 Y with Emax. 2,28% MeV is 0,5%. The VYNS absorptions were determined by counting using variations of VYNS thickness. The corrections of VYNS are 0,70% at Eeta max. 0,55 MeV and 0,1% at Ebeta max. 2,28 MeV, for every VYNS sheet having thickness of n 15 mugr/cm exp.2. The samples were made from the source without dilution, and with dilution factors of 6,9 and 9,9. The activity of samples were determined by counting using two methods, with and without variation of VYNS thickness. The result of measurement by variation dilution of solution and VYNS thickness is fairly good, the difference is 1,68%. The difference between result of activity measurement of exp.90 Sr with this methods and the activity of master solution is fairly good, with the discrepancies are from 0,5 to 1,16%

  13. Determination of {sup 90}Sr Abundance in Different Kind of Pasture at PPTN Serpong; Distribusi {sup 90}Sr dalam Beberapa Jenis Rumput di Kawasan PPTN Serpong

    Energy Technology Data Exchange (ETDEWEB)

    Sudiyati,; Makmur, Murdahayu; S, Agus Gindo [Centre for Radioactive Waste Technology, National Nuclear Energy Agency, Serpong (Indonesia)

    2007-06-15

    The determination of {sup 90}Sr abundance in the pasture grass around Serpong had been done. The aim of the research is to determine {sup 90}Sr abundance in the pasture which could be used to estimate the dose received by human in food chain pathways. The mean of {sup 90}Sr mean activities in pasture around PPTN Serpong is 14.14 ± 3.7 mBq/kg, this result is insignificantly different compared to its control (off-site), i.e. 14.18 ± 6.9 mBq/kg. Elephant grass (Penisettum Purpureum) has the highest abundance of {sup 90}Sr which is 24.8 ± 15.6 mBq/kg, followed by teki grass (Setaria Spha Celata) and ilalang grass (Imperata Cylinrica) which are 14.9 ± 6.0 mBq/kg and 8.0 ± 4.8 mBq/kg, respectively. (author)

  14. Heat shock protein 90 (Hsp90) chaperone complex. A molecular target for enhancement of thermosensitivity and radiosensitivity

    International Nuclear Information System (INIS)

    Akimoto, Tetsuo; Nonaka, Tetsuo; Kitamoto, Yoshizumi; Sakurai, Hideyuki

    2002-01-01

    Heat shock protein 90 (Hsp90) is a highly conserved heat shock protein in animal and plants, and exists abundantly in the cytoplasm in unstressed condition, accounting for 1-2% in cytoplasmic proteins. Main difference of Hsp90 from other Hsps are its substrate that Hsp90 binds to. These substrates include various signal transduction proteins, kinase, steroid receptors and transcription factors, therefore, Hsp90 plays a key role in maintaining cellular signal transduction networks. Many chaperoned proteins (client proteins) of Hsp90 are associated with cellular proliferation or malignant transformation, thus Hsp90 chaperone complex has been focused as targets for cancer therapy. Among the client proteins, there are several molecules that have been defined as targets or factors for determination or enhancement of radiosensitivity or thermosensitivity. Thus, it is easily speculated that Hsp90 chaperone complex inhibitors that disrupt association of Hsp90 and client protein in combination with radiation or/and heat has potential effect on enhancement of radiosensitivity or thermosensitivity. In this paper, possible mechanisms in enhancing radiosensitivity or thermosensitivity according to the client proteins will be summarized. (author)

  15. Chemical compositions and antimicrobial activity of twig essential ...

    African Journals Online (AJOL)

    Aghomotsegin

    2016-03-09

    Mar 9, 2016 ... The chemical composition of twig essential oils of Xylopia malayana, Xylopia elliptica and Xylopia fusca ... Volatile constituents and bioactivity studies are available in the literature on Xylopia aethiopica (Issakou et al., 2014;. Sylvain et al, 2014; Vyry et al, 2014), Xylopia longifolia. (Fourier et al, 1993), ...

  16. A case of internal contamination by 90Sr of a member of public

    International Nuclear Information System (INIS)

    Malatova, I.; Cespirova, I.; Drabova, D.; Beckova, V.

    1998-01-01

    A case of internal contamination by 90 Sr of a member of the public is described. An unauthorised person entered a low-activity waste storage placed in an abandoned sand mine, a barrel with marker for radioactive substances was found and manipulated with a tin labelled as ISOMET Sr90. The radiation protection authority was informed after 6 days and found surface contamination on his belongings. Whole-body counting with an HPGe detector and excretion analysis were performed. He was measured by whole-body counter 16 times from November 1990 to August 1992, 9 samples of urine and 6 samples of faeces were collected. For the estimation of the dose, the software CINDY with the biokinetic models of ICRP 30 was used. Single and continuous intakes were assumed. Intake was estimated to be about 3 MBq and committed effective dose 84 mSv. (author)

  17. Glutamine reduces myocardial cell apoptosis in a rat model of sepsis by promoting expression of heat shock protein 90.

    Science.gov (United States)

    Li, Wanxia; Tao, Shaoyu; Wu, Qinghua; Wu, Tao; Tao, Ran; Fan, Jun

    2017-12-01

    Myocardial cell injury and cardiac myocyte apoptosis are associated with sepsis. Glutamine (Gln) has been reported to repair myocardial cell injury. The aim of this study was to explore the role of Gln on cardiac myocytes in a cecal ligation and puncture (CLP) model of sepsis in Wistar rats. Following induction of sepsis in a CLP rat model, viral encoding heat shock protein 90 (Hsp90) gene and Hsp90dsDNA were designed to express and knockdown Hsp90, respectively. Rat cardiac tissues were examined histologically, and apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling staining. The expression of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein, Hsp90, p53 upregulated modulator of apoptosis, and p53 was measured by western blotting and real-time polymerase chain reaction. Caspase-3, caspase-8, and caspase-9 were detected by enzyme-linked immunosorbent assay. Rat cardiac myocyte damage induced by CLP was reduced by Gln treatment and Hsp90 overexpression, and these changes were reversed by Hsp90 knockdown. Bcl-2 expression, Bcl-2-associated X protein, p53, p53 upregulated modulator of apoptosis, caspase-8, caspase-9, and caspase-3 activities were significantly upregulated in the CLP model, which were reduced by Gln treatment and Hsp90 overexpression. Gln reduced apoptosis of cardiac myocytes in a rat model of sepsis, by promoting Hsp90 expression. Further studies are needed to determine the possible therapeutic action of Gln in sepsis in human tissue. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. An estimate of equatorial wave energy flux at 9- to 90-day periods in the Central Pacific

    Science.gov (United States)

    Eriksen, Charles C.; Richman, James G.

    1988-01-01

    Deep fluctuations in current along the equator in the Central Pacific are dominated by coherent structures which correspond closely to narrow-band propagating equatorial waves. Currents were measured roughly at 1500 and 3000 m depths at five moorings between 144 and 148 deg W from January 1981 to March 1983, as part of the Pacific Equatorial Ocean Dynamics program. In each frequency band resolved, a single complex empirical orthogonal function accounts for half to three quarters of the observed variance in either zonal or meridional current. Dispersion for equatorial first meridional Rossby and Rossby gravity waves is consistent with the observed vertical-zonal coherence structure. The observations indicate that energy flux is westward and downward in long first meridional mode Rossby waves at periods 45 days and longer, and eastward and downward in short first meridional mode Rossby waves and Rossby-gravity waves at periods 30 days and shorter. A local minimum in energy flux occurs at periods corresponding to a maximum in upper-ocean meridional current energy contributed by tropical instability waves. Total vertical flux across the 9- to 90-day period range is 2.5 kW/m.

  19. Phylogenetic relationships among Brazilian howler monkeys, genus Alouatta (Platyrrhini, Atelidae, based on g1-globin pseudogene sequences

    Directory of Open Access Journals (Sweden)

    Carla Maria Meireles

    1999-09-01

    Full Text Available The genus Alouatta (howler monkeys is the most widely distributed of New World primates, and has been arranged in three species groups: the Central American Alouatta palliata group and the South American Alouatta seniculus and Alouatta caraya groups. While the latter is monotypic, the A. seniculus group encompasses at least three species (A. seniculus, A. belzebul and A. fusca. In the present study, approximately 600 base pairs of the g1-globin pseudogene were sequenced in the four Brazilian species (A. seniculus, A. belzebul, A. fusca and A. caraya. Maximum parsimony and maximum likelihood methods yielded phylogenetic trees with the same arrangement: {A. caraya [A. seniculus (A. fusca, A. belzebul]}. The most parsimonious tree had bootstrap values greater than 82% for all groupings, and strength of grouping values of at least 2, supporting the sister clade of A. fusca and A. belzebul. The study also confirmed the presence of a 150-base pair Alu insertion element and a 1.8-kb deletion in the g1-globin pseudogene in A. fusca, features found previously in the remaining three species. The cladistic classification based on molecular data agrees with those of morphological studies, with the monospecific A. caraya group being clearly differentiated from the A. seniculus group.Os guaribas, do gênero Alouatta, que são os primatas do Novo Mundo com maior distribuição geográfica, têm sido colocados em três grupos de espécies: o grupo Alouatta palliata da América central, e os grupos sulamericanos Alouatta seniculus e Alouatta caraya. Este último é monotípico, mas o grupo A. seniculus inclui pelo menos três espécies (A. seniculus, A. belzebul e A. fusca. Neste estudo, foram seqüenciados aproximadamente 600 pares de base do pseudogene globina g1 nas quatro espécies brasileiras (A. seniculus, A. belzebul, A. fusca e A. caraya. Os métodos de máxima parcimônia e máxima verossimilhança produziram árvores filogenéticas com o mesmo arranjo

  20. Hanford isotope project strategic business analysis yttrium-90 (Y-90)

    International Nuclear Information System (INIS)

    1995-10-01

    The purpose of this analysis is to address the short-term direction for the Hanford yttrium-90 (Y-90) project. Hanford is the sole DOE producer of Y-90, and is the largest repository for its source in this country. The production of Y-90 is part of the DOE Isotope Production and Distribution (IP and D) mission. The Y-90 is ''milked'' from strontium-90 (Sr-90), a byproduct of the previous Hanford missions. The use of Sr-90 to produce Y-90 could help reduce the amount of waste material processed and the related costs incurred by the clean-up mission, while providing medical and economic benefits. The cost of producing Y-90 is being subsidized by DOE-IP and D due to its use for research, and resultant low production level. It is possible that the sales of Y-90 could produce full cost recovery within two to three years, at two curies per week. Preliminary projections place the demand at between 20,000 and 50,000 curies per year within the next ten years, assuming FDA approval of one or more of the current therapies now in clinical trials. This level of production would incentivize private firms to commercialize the operation, and allow the government to recover some of its sunk costs. There are a number of potential barriers to the success of the Y-90 project, outside the control of the Hanford Site. The key issues include: efficacy, Food and Drug Administration (FDA) approval and medical community acceptance. There are at least three other sources for Y-90 available to the US users, but they appear to have limited resources to produce the isotope. Several companies have communicated interest in entering into agreements with Hanford for the processing and distribution of Y-90, including some of the major pharmaceutical firms in this country

  1. Hanford isotope project strategic business analysis yttrium-90 (Y-90)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-10-01

    The purpose of this analysis is to address the short-term direction for the Hanford yttrium-90 (Y-90) project. Hanford is the sole DOE producer of Y-90, and is the largest repository for its source in this country. The production of Y-90 is part of the DOE Isotope Production and Distribution (IP and D) mission. The Y-90 is ``milked`` from strontium-90 (Sr-90), a byproduct of the previous Hanford missions. The use of Sr-90 to produce Y-90 could help reduce the amount of waste material processed and the related costs incurred by the clean-up mission, while providing medical and economic benefits. The cost of producing Y-90 is being subsidized by DOE-IP and D due to its use for research, and resultant low production level. It is possible that the sales of Y-90 could produce full cost recovery within two to three years, at two curies per week. Preliminary projections place the demand at between 20,000 and 50,000 curies per year within the next ten years, assuming FDA approval of one or more of the current therapies now in clinical trials. This level of production would incentivize private firms to commercialize the operation, and allow the government to recover some of its sunk costs. There are a number of potential barriers to the success of the Y-90 project, outside the control of the Hanford Site. The key issues include: efficacy, Food and Drug Administration (FDA) approval and medical community acceptance. There are at least three other sources for Y-90 available to the US users, but they appear to have limited resources to produce the isotope. Several companies have communicated interest in entering into agreements with Hanford for the processing and distribution of Y-90, including some of the major pharmaceutical firms in this country.

  2. Molecular cloning, purification, expression, and characterization of β-1, 4-endoglucanase gene ( from sp. isolated from Holstein steers’ rumen

    Directory of Open Access Journals (Sweden)

    Tansol Park

    2018-04-01

    Full Text Available Objective This study was conducted to isolate the cellulolytic microorganism from the rumen of Holstein steers and characterize endoglucanase gene (Cel5A from the isolated microorganism. Methods To isolate anaerobic microbes having endoglucanase, rumen fluid was obtained from Holstein steers fed roughage diet. The isolated anaerobic bacteria had 98% similarity with Eubacterium cellulosolvens (E. cellulosolvens Ce2 (Accession number: AB163733. The Cel5A from isolated E. cellulolsovens sp. was cloned using the published genome sequence and expressed through the Escherichia coli BL21. Results The maximum activity of recombinant Cel5A (rCel5A was observed at 50°C and pH 4.0. The enzyme was constant at the temperature range of 20°C to 40°C but also, at the pH range of 3 to 9. The metal ions including Ca2+, K+, Ni2+, Mg2+, and Fe2+ increased the endoglucanase activity but the addition of Mn2+, Cu2+, and Zn2+ decreased. The Km and Vmax value of rCel5A were 14.05 mg/mL and 45.66 μmol/min/mg. Turnover number, Kcat and catalytic efficiency, Kcat/Km values of rCel5A was 96.69 (s−1 and 6.88 (mL/mg/s, respectively. Conclusion Our results indicated that rCel5A of E. cellulosolvens isolated from Holstein steers had a broad pH range with high stability under various conditions, which might be one of the beneficial characteristics of this enzyme for possible industrial application.

  3. Widespread seismicity excitation throughout central Japan following the 2011 M=9.0 Tohoku earthquake and its interpretation by Coulomb stress transfer

    Science.gov (United States)

    Toda, S.; Stein, R.S.; Lin, J.

    2011-01-01

    We report on a broad and unprecedented increase in seismicity rate following the M=9.0 Tohoku mainshock for M ≥ 2 earthquakes over inland Japan, parts of the Japan Sea and Izu islands, at distances of up to 425 km from the locus of high (≥15 m) seismic slip on the megathrust. Such an increase was not seen for the 2004 M=9.1 Sumatra or 2010 M=8.8 Chile earthquakes, but they lacked the seismic networks necessary to detect such small events. Here we explore the possibility that the rate changes are the product of static Coulomb stress transfer to small faults. We use the nodal planes of M ≥ 3.5 earthquakes as proxies for such small active faults, and find that of fifteen regions averaging ~80 by 80 km in size, 11 show a positive association between calculated stress changes and the observed seismicity rate change, 3 show a negative correlation, and for one the changes are too small to assess. This work demonstrates that seismicity can turn on in the nominal stress shadow of a mainshock as long as small geometrically diverse active faults exist there, which is likely quite common.

  4. Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent

    Directory of Open Access Journals (Sweden)

    Thaís V. Souza

    2016-03-01

    Full Text Available CelE1 is a cold-active endo-acting glucanase with high activity at a broad temperature range and under alkaline conditions. Here, we examined the effects of pH on the secondary and tertiary structures, net charge, and activity of CelE1. Although variation in pH showed a small effect in the enzyme structure, the activity was highly influenced at acidic conditions, while reached the optimum activity at pH 8. Furthermore, to estimate whether CelE1 could be used as detergent additives, CelE1 activity was evaluated in the presence of surfactants. Ionic and nonionic surfactants were not able to reduce CelE1 activity significantly. Therefore, CelE1 was found to be promising candidate for use as detergent additives. Finally, we reported a thermodynamic analysis based on the structural stability and the chemical unfolding/refolding process of CelE1. The results indicated that the chemical unfolding proceeds as a reversible two-state process. These data can be useful for biotechnological applications.

  5. 90Sr content in 90Y-labeled SIR-spheres and Zevalin.

    Science.gov (United States)

    Metyko, John; Erwin, William; Poston, John; Jimenez, Sandra

    2014-11-01

    Three different 90Y internally administered radionuclide therapies are currently used in both standard-of-care and clinical trial procedures atMD Anderson Cancer Center. TheraSphere and SIR-Spheres therapies utilize 90Y-labeled microspheres, while Zevalin is an 90Y-labeled radioimmunotherapeutic agent. Several publications have indicated radionuclidic impurities resulting from 90Y production methods. The 90Y in SIR-Spheres and Zevalin are produced from a 90Sr/90Y generator, which leaves measurable quantities of 90Sr in the final product. TheraSphere 90Y is produced in a nuclear reactor which results in a large number of impurities, most notably 88Y and 91Y. Product information sheets reference these impurities with specific limits given. These limits represent a tiny fraction of the total product activity, and in the case of TheraSphere and SIR-Spheres gamma-emitting impurities, this has been verified in the literature. An analysis of 90Sr impurities in SIR-Spheres and Zevalin is presented in this paper. Impurity quantities were found to be within the vendors’ documented limits.

  6. Desarrollo de producto sobre la base de harinas de cereales y leguminosa para niños celíacos entre 6 y 24 meses; I: Formulación y aceptabilidad

    OpenAIRE

    Cerezal Mezquita, P.; Urtuvia Gatica, V.; Ramírez Quintanilla, V.; Romero Palacios, N.; Arcos Zavala, R.

    2011-01-01

    La revalorización de los cultivos andinos, quinua (Chenopodium quinua Willd) y lupino (Lupinus albus L), para ser utilizados en mezclas alimenticias, con cereales tradicionales como maíz (Zea mays L.) y arroz (Oryza sativa L.), originan mezclas sin gluten que constituyen una buena alternativa para la alimentación de niños menores de 24 meses que sufren la enfermedad celíaca, ya que mejoran la calidad de la proteína, por compensación de los aminoácidos esenciales, e incide en la diversificació...

  7. Nutritional Value And Yield of Kallar Grass (Leptochloa Fusca Linn) Hay and Its Effect on Reproductive Performance of Awassi Ewes Using Progesterone Radioimmunoassay

    International Nuclear Information System (INIS)

    Al-Masri, M R..; Zarkawi, M.; Khalifa, K.

    2007-01-01

    Nutritional value of Kallar grass (Leptochloa fusca Linn) hay (whole plant), a salt-tolerant plant, was estimated by determinning its composition (ash, crude protein CP, crude fibre CF, ether extract EE, neutral-detergent fiber NDF, acid-detergent fiber ADF, acid-detergent lignin ADL); and the in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), net energy of lactation (NEL) and gross energy (GE). Moreover, dry matter, energy and protein yields per hectar were also estimated. The effects of feeding Kallar grass hay to pregnant Awassi ewes on reproductive performance, serum progesterone changes using radioimmunoassay, and on birth and weaning weights of lambs born were also determined. Experimental ewes received daily 300 g of Kallar grass hay in addition to lentil straw and concentrates to cover their nutritional requirements. The results indicated that the nutritive components of Kallar grass were (g kg -1 dry matter): ash 96, CP 93, CF 290, EE 21, NDF 755, ADF 416 and ADL 94. IVOMD was 49.3%, and energy values (MJ kg -1 dry matter) were: GE 26.88, ME 6.41 and NEL 3.02. Dry matter and crude protein yields of Kallar grass hay were 7875 and 732 kg ha -1 , respectively, and the energy produced (MJ ha -1 ) was: GE 211680, ME 50479 and NEL 23783. Kallar grass had no effects on the reproductive performance of pregnant Awassi ewes duration, of pregnancy (150.1 ± 2.2 days), progesterone patterns, birth (4.7±1.0 kg) or on weaning weights (22.7 ± ≤ 5.5 kg) of lambs.

  8. Bacterial endophytes enhance phytostabilization in soils contaminated with uranium and lead.

    Science.gov (United States)

    Ahsan, Muhammad Tayyab; Najam-Ul-Haq, Muhammad; Idrees, Muhammad; Ullah, Inayat; Afzal, Muhammad

    2017-10-03

    The combined use of plants and bacteria is a promising approach for the remediation of polluted soil. In the current study, the potential of bacterial endophytes in partnership with Leptochloa fusca (L.) Kunth was evaluated for the remediation of uranium (U)- and lead (Pb)-contaminated soil. L. fusca was vegetated in contaminated soil and inoculated with three different endophytic bacterial strains, Pantoea stewartii ASI11, Enterobacter sp. HU38, and Microbacterium arborescens HU33, individually as well as in combination. The results showed that the L. fusca can grow in the contaminated soil. Bacterial inoculation improved plant growth and phytoremediation capacity: this manifested in the form of a 22-51% increase in root length, 25-62% increase in shoot height, 10-21% increase in chlorophyll content, and 17-59% more plant biomass in U- and Pb-contaminated soils as compared to plants without bacterial inoculation. Although L. fusca plants showed potential to accumulate U and Pb in their root and shoot on their own, bacterial consortia further enhanced metal uptake capacity by 53-88% for U and 58-97% for Pb. Our results indicate that the combination of L. fusca and endophytic bacterial consortia can effectively be used for the phytostabilization of both U- and Pb-contaminated soils.

  9. Same-day {sup 90}Y radioembolization: implementing a new treatment paradigm

    Energy Technology Data Exchange (ETDEWEB)

    Gabr, Ahmed; Kallini, Joseph Ralph; Gates, Vanessa L.; Hickey, Ryan; Desai, Kush; Thornburg, Bartley; Marshall, Karen; Salzig, Krystina; Williams, Melissa; Del Castillo, Carlene; Hohlastos, Elias; Lewandowski, Robert J. [Northwestern Memorial Hospital, Robert H. Lurie Comprehensive Cancer Center, Department of Radiology, Section of Interventional Radiology, Chicago, IL (United States); Kulik, Laura; Ganger, Daniel [Northwestern University, Department of Medicine, Division of Hepatology, Chicago, IL (United States); Baker, Talia [Northwestern University, Department of Surgery, Division of Transplantation, Comprehensive Transplant Center, Chicago, IL (United States); Salem, Riad [Northwestern Memorial Hospital, Robert H. Lurie Comprehensive Cancer Center, Department of Radiology, Section of Interventional Radiology, Chicago, IL (United States); Northwestern University, Department of Surgery, Division of Transplantation, Comprehensive Transplant Center, Chicago, IL (United States); Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Department of Medicine, Division of Hematology and Oncology, Chicago, IL (United States)

    2016-12-15

    To assess the feasibility of conducting pretreatment mesenteric angiography, coil embolization, {sup 99m}Tc macroaggregated albumin ({sup 99m}Tc-MAA) scintigraphy, and {sup 90}Y radioembolization treatment in a single, same-day, combined outpatient encounter. This was a retrospective study of 78 patients treated during the period 2008 - 2015 who were managed in a single outpatient encounter under the guidance of the Interventional Radiology Department and The Nuclear Medicine Department. Pretreatment planning was performed by reviewing baseline imaging and estimated perfused liver volume bearing the tumor. The region of interest was estimated using 3-D software; this value was used for dosimetry planning. Maximum lung shunting fractions of 10 % for hepatocellular carcinoma and 5 % for liver metastases were assumed. Subsequently, hepatic angiography and {sup 99m}Tc-MAA scintigraphy were performed followed by {sup 90}Y treatment in one outpatient encounter. Total in-room procedure time was recorded. All patients underwent same-day angiography, {sup 99m}Tc-MAA scintigraphy and {sup 90}Y radioembolization. Of the 78 patients, 16 received multiple segmental treatments to both lobes, 44 received treatment to the right lobe, and 18 received treatment to the left lobe. The median dose was 106 Gy. The median number of {sup 90}Y vials needed was two (range one to six). The median in-room time was 160 min (75 - 250 min). The residential status of the patients was as follows, 18 % (14/78) were local residents, 55 % (43/78) traveled from outside the city limits, 18 % (14/78) were from out-of-state, and 9 % (7/78) were resident abroad. Of the 78 patients, 61 (77 %) had hepatocellular carcinoma, and 17 (22 %) had liver metastases. The median lung dose was 3.5 Gy. This study demonstrated the feasibility of same-day {sup 90}Y evaluation and treatment while maintaining the principles of safe and effective {sup 90}Y infusion including tumoricidal dosimetry (lobar, segmentectomy

  10. Simultaneous ultra-long data retention and low power based on Ge10Sb90/SiO2 multilayer thin films

    Science.gov (United States)

    You, Haipeng; Hu, Yifeng; Zhu, Xiaoqin; Zou, Hua; Song, Sannian; Song, Zhitang

    2018-02-01

    In this article, Ge10Sb90/SiO2 multilayer thin films were prepared to improve thermal stability and data retention for phase change memory. Compared with Ge10Sb90 monolayer thin film, Ge10Sb90 (1 nm)/SiO2 (9 nm) multilayer thin film had higher crystallization temperature and resistance contrast between amorphous and crystalline states. Annealed Ge10Sb90 (1 nm)/SiO2 (9 nm) had uniform grain with the size of 15.71 nm. After annealing, the root-mean-square surface roughness for Ge10Sb90 (1 nm)/SiO2 (9 nm) thin film increased slightly from 0.45 to 0.53 nm. The amorphization time for Ge10Sb90 (1 nm)/SiO2 (9 nm) thin film (2.29 ns) is shorter than Ge2Sb2Te5 (3.56 ns). The threshold voltage of a cell based on Ge10Sb90 (1 nm)/SiO2 (9 nm) (3.57 V) was smaller than GST (4.18 V). The results indicated that Ge10Sb90/SiO2 was a promising phase change thin film with high thermal ability and low power consumption for phase change memory application.

  11. Radiolabeling Of Albumin Particles With Yttrium-90

    International Nuclear Information System (INIS)

    Nguyen Thi Thu; Nguyen Thi Khanh Giang; Bui Van Cuong, Vo Thi Cam Hoa

    2011-01-01

    This paper presents the process of the radiolabeling of microaggregated albumin particles with radionuclide Yttrium-90 using the directed method. The albumin microsphere kit was prepared in sodium phosphate buffer. The original solution includes 2 mg albumin particle and 0.5 mg stannous chloride dihydrate. The albumin particles size was ranged from 5 ?m to 30 ?m. The mixture was washed three times with phosphate buffer saline, pH 7.2 by centrifugation and suspended in 0.5 M sodium acetate buffer, pH 6. Yttrium - 90 in 1.0 M acetic acid was collected from 90 Sr/ 90 Y generator. The labeling of the particles with Y-90 (185 MBq) was performed at pH 5.5 in acetate buffer with agitating for 60 min at room temperature. The labeled albumin suspensions were centrifuged at 3000 rpm for 15 min. Labeling yields was calculated using centrifugation, filtration and compared with paper chromatography, which is developed in the Tris Acetic EDTA. In this system, the unbound of Y-90 migrates to an R f of 0.9-1.0 and the radiolabeled albumin particles remains at the point of origin (R f = 0). The size of 90 Y-albumin particles was compared with the albumin particles in the original solution to be sure that they did not change during the labeling treatment. The radiolabeling yields were more than 80%. The labeled compound was dialysis in phosphate buffer. The radiochemical purity was 98%. The 90 Y- albumin is an ideal radiopharmaceutical for potential use in malignant cancer treatment as brachytherapy. (author)

  12. Electron spin resonance of Gd3+ in the intermetallic Gd1-xYxNi3Ga9 (0 ≤ x ≤ 0.90) compounds

    Science.gov (United States)

    Mendonça, E. C.; Silva, L. S.; Mercena, S. G.; Meneses, C. T.; Jesus, C. B. R.; Duque, J. G. S.; Souza, J. C.; Pagliuso, P. G.; Lora-Serrano, R.; Teixeira-Neto, A. A.

    2017-10-01

    In this work, experiments of X-ray diffraction, magnetic susceptibility, heat capacitance, and Electron Spin Resonance (ESR) carried out in the Gd1-xYxNi3Ga9 (0 ≤ x ≤ 0.90) compounds grown through a Ga self flux method are reported. The X-ray diffraction data indicate that these compounds crystallize in a trigonal crystal structure with a space group R32. This crystal structure is unaffected by Y-substitution, which produces a monotonic decrease of the lattice parameters. For the x = 0 compound, an antiferromagnetic phase transition is observed at TN = 19.2 K, which is continuously suppressed as a function of the Y-doping and extrapolates to zero at x ≈ 0.85. The ESR data, taken in the temperature range 15 ≤ T ≤ 300 K, show a single Dysonian Gd3+ line with nearly temperature independent g-values. The linewidth follows a Korringa-like behavior as a function of temperature for all samples. The Korringa rates (b = ΔH /ΔT ) are Y-concentration-dependent indicating a "bottleneck" regime. For the most diluted sample (x = 0.90), when it is believed that the "bottleneck" effect is minimized, we have calculated the q-dependent effective exchange interactions between Gd3+ local moments and the c-e of 〈Jf-ce 2(q ) 〉 1 /2 = 18(2) meV and Jf -c e(q =0 ) = 90(10) meV.

  13. Cesium - 137 and strontium -90 in dairy-milk. First quarter 1981

    International Nuclear Information System (INIS)

    1981-05-01

    Results of mesurements of 137 Cs and 90 Sr activities in milk at 9 dairies in Sweden during the first three months of 1981. Meanvalues for all dairies are: 137 Cs - 0.15 Bq/l, 90 Sr - 0.12 Bq/l. The regional variations are considerable. (L.E.)

  14. Mesoporous carbon with spherical pores as a carrier for celecoxib with needle-like crystallinity: Improve dissolution rate and bioavailability

    International Nuclear Information System (INIS)

    Zhu, Wenquan; Zhao, Qinfu; Sun, Changshan; Zhang, Zhiwen; Jiang, Tongying; Sun, Jin; Li, Yaping; Wang, Siling

    2014-01-01

    The purposes of this investigation are to design mesoporous carbon (MC) with spherical pore channels and incorporate CEL to it for changing its needlelike crystal form and improving its dissolution and bioavailability. A series of solid-state characterization methods, such as SEM, TEM, DSC and XRD, were employed to systematically investigate the existing status of celecoxib (CEL) within the pore channels of MC. The pore size, pore volume and surface area of samples were characterized by nitrogen physical absorption. Gastric mucosa irritation test was carried out to evaluate the safety of mesoporous carbon as a drug carrier. Dissolution tests and in vivo pharmacokinetic studies were conducted to confirm the improvement in drug dissolution kinetics and oral bioavailability. Uptake experiments were conducted to investigate the mechanism of the improved oral bioavailability. The results of solid state characterization showed that MC was prepared successfully and CEL was incorporated into the mesoporous channels of the MC. The crystallinity of CEL in MC was affected by different loading methods, which involve evaporation method and melting method. The dissolution rate of CEL from MC was found to be significantly higher than that of pure CEL, which attributed to reduced crystallinity of CEL. The gastric mucosa irritation test indicated that the MC caused no harm to the stomach and produced a protective effect on the gastric mucosa. Uptake experiments indicated that MC enhanced the amount of CEL absorbed by Caco-2 cells. Moreover, oral bioavailability of CEL loaded within the MC was approximately 1.59-fold greater than that of commercial CEL. In conclusion, MC was a safe carrier to load water insoluble drug by controlling the crystallinity or crystal form with improvement in drug dissolution kinetics and oral bioavailability. - Highlights: • Mesoporous carbon with spherical pore structure was prepared according to the needlelike crystalline of celecoxib. • The

  15. Mesoporous carbon with spherical pores as a carrier for celecoxib with needle-like crystallinity: Improve dissolution rate and bioavailability

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Wenquan; Zhao, Qinfu; Sun, Changshan [Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang (China); Zhang, Zhiwen [Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203 (China); Jiang, Tongying; Sun, Jin [Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang (China); Li, Yaping [Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203 (China); Wang, Siling, E-mail: silingwang@syphu.edu.cn [Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang (China)

    2014-06-01

    The purposes of this investigation are to design mesoporous carbon (MC) with spherical pore channels and incorporate CEL to it for changing its needlelike crystal form and improving its dissolution and bioavailability. A series of solid-state characterization methods, such as SEM, TEM, DSC and XRD, were employed to systematically investigate the existing status of celecoxib (CEL) within the pore channels of MC. The pore size, pore volume and surface area of samples were characterized by nitrogen physical absorption. Gastric mucosa irritation test was carried out to evaluate the safety of mesoporous carbon as a drug carrier. Dissolution tests and in vivo pharmacokinetic studies were conducted to confirm the improvement in drug dissolution kinetics and oral bioavailability. Uptake experiments were conducted to investigate the mechanism of the improved oral bioavailability. The results of solid state characterization showed that MC was prepared successfully and CEL was incorporated into the mesoporous channels of the MC. The crystallinity of CEL in MC was affected by different loading methods, which involve evaporation method and melting method. The dissolution rate of CEL from MC was found to be significantly higher than that of pure CEL, which attributed to reduced crystallinity of CEL. The gastric mucosa irritation test indicated that the MC caused no harm to the stomach and produced a protective effect on the gastric mucosa. Uptake experiments indicated that MC enhanced the amount of CEL absorbed by Caco-2 cells. Moreover, oral bioavailability of CEL loaded within the MC was approximately 1.59-fold greater than that of commercial CEL. In conclusion, MC was a safe carrier to load water insoluble drug by controlling the crystallinity or crystal form with improvement in drug dissolution kinetics and oral bioavailability. - Highlights: • Mesoporous carbon with spherical pore structure was prepared according to the needlelike crystalline of celecoxib. • The

  16. Photofission cross-section ratio measurement of {sup 235}U/{sup 238}U using monoenergetic photons in the energy range of 9.0–16.6 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Krishichayan, E-mail: krishi@tunl.duke.edu [Department of Physics, Duke University, Durham, NC 27708 (United States); Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States); Bhike, Megha; Finch, S.W.; Howell, C.R. [Department of Physics, Duke University, Durham, NC 27708 (United States); Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States); Tonchev, A.P. [Nuclear and Chemical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550 (United States); Tornow, W. [Department of Physics, Duke University, Durham, NC 27708 (United States); Triangle Universities Nuclear Laboratory, Durham, NC 27708 (United States)

    2017-05-11

    Photofission cross-section ratios of {sup 235}U and {sup 238}U have been measured using monoenergetic photon beams at the HIγS facility of TUNL. These measurements have been performed in small energy steps between 9.0 and 16.6 MeV using a dual-fission ionization chamber. Measured cross-section ratios are compared with the previous experimental data as well as with the recent evaluated nuclear data library ENDF.

  17. Country report: Vietnam. Setting Up of a 90Sr/90Y Generator System Based on Supported Liquid Membrane (SLM) Technique and Radiolabeling of Eluted 90Y with Biomolecules

    International Nuclear Information System (INIS)

    Nguyen Thi Thu; Duong Van Dong; Bui Van Cuong; Chu Van Khoa

    2010-01-01

    In the course of participating in the IAEA-CRP during the last two years, Vietnam has achieved the goal of setting up a 90 Sr/ 90 Y generator system based on Supported Liquid Membrane (SLM) technique and also radiolabeling of the eluted 90 Y with antibody, peptides and albumin. A two stage SLM based 90 Sr- 90 Y generator was set up in-house to generate carrier-free 90 Y at different activity levels viz. 5, 20, 50 mCi. The generator system was operated in sequential mode in which 2-ethylhexyl 2-ethylhexyl phosphonic acid (PC88A) based SLM was used in the first stage for the transport 90 Y in 4.0 M nitric acid from source phase where 90 Sr- 90 Y equilibrium mixture is placed in nitric acid medium at pH to 1-2. In the second stage, octyl (phenyl)-N,N-diisobutylcarbamoylmethyl phosphine oxide (CMPO) based SLM was used for the transport of 90 Y selectively to 1.0 M acetic acid which is the best medium for radiolebeling. The eluted 90 Y from the generator was tested for the presence of any traces of 90 Sr using the Extraction Paper Chromatography (EPC) and was found suitable for radiolabeling. The generator system could be upgraded to 100 mCi level successfully due to an expert mission from India through IAEA. The 90 Y product obtained from the generator system was used for radiolabeling of antibody and peptides viz. Rituximab, DOTATATE and albumin particles under different experimental conditions. A new chromatography system could be developed for analyzing 90 Y labeled albumin using the TAE buffer as mobile phase in PC and ITLC

  18. Country report: Thailand. Development of Sr-90/ Y-90 Generator and Development of Radiopharmaceuticals Using Y-90

    International Nuclear Information System (INIS)

    Nipavan, Poramatikul

    2010-01-01

    The research project has been conducted at Thailand Institute of Nuclear Technology in accordance to the 1st RCM plan during the IAEA meeting in Warsaw. The objectives of the project include the following 5 specific aims: 1. Development of Sr-90/Y-90 ion-exchange chromatography generator 2. Development of Sr-90/Y-90 extraction chromatography generator 3. Development of quality control technique 4. Development of herapeutic radiopharmaceuticals Y-90 particulates/colloids 5. Development of Re-188 DMSA–bis-phosphonates Currently we have achieved specific aims 1 to 3. The specific aims 4 and 5 are during investigation. For specific aim 4, we are during the process to extract high purity 90 Y from 90 Sr/ 90 Y generator that will yield the starting 90 Y for the production of Y-90 particulates and colloids. For the 5 th specific aim, we are on hold to receive the starting agent, bis- Phosphonates, from Dr. Blower group. Therefore, this progress report will cover our work focusing on specific aims 1 to 3

  19. Country report: Thailand. Development of Sr-90/ Y-90 Generator and Development of Radiopharmaceuticals Using Y-90

    Energy Technology Data Exchange (ETDEWEB)

    Nipavan, Poramatikul [Nuclear Research and Development Group, Thailand Institute of Nuclear Technology (Public Organization), Bangkok (Thailand)

    2010-07-01

    The research project has been conducted at Thailand Institute of Nuclear Technology in accordance to the 1st RCM plan during the IAEA meeting in Warsaw. The objectives of the project include the following 5 specific aims: 1. Development of Sr-90/Y-90 ion-exchange chromatography generator 2. Development of Sr-90/Y-90 extraction chromatography generator 3. Development of quality control technique 4. Development of herapeutic radiopharmaceuticals Y-90 particulates/colloids 5. Development of Re-188 DMSA–bis-phosphonates Currently we have achieved specific aims 1 to 3. The specific aims 4 and 5 are during investigation. For specific aim 4, we are during the process to extract high purity {sup 90}Y from {sup 90}Sr/{sup 90}Y generator that will yield the starting {sup 90}Y for the production of Y-90 particulates and colloids. For the 5{sup th} specific aim, we are on hold to receive the starting agent, bis- Phosphonates, from Dr. Blower group. Therefore, this progress report will cover our work focusing on specific aims 1 to 3.

  20. The 90 90 90 strategy to end the HIV Pandemic by 2030: Can the supply chain handle it?

    Science.gov (United States)

    Jamieson, David

    2016-01-01

    Introduction UNAIDS “90-90-90” strategy calls for 90% of HIV-infected individuals to be diagnosed by 2020, 90% of whom will be on anti-retroviral therapy (ART) and 90% of whom will achieve sustained virologic suppression. Reaching these targets by 2020 will reduce the HIV epidemic to a low-level endemic disease by 2030. However, moving the global response towards this universal test and treat model will pose huge challenges to public health systems in resource-limited settings, including global and local supply chain systems. These challenges are especially acute in Africa, which accounts for over 70% of the persons affected by HIV. Discussion From a supply chain perspective, each of the “90's” has possible complications and roadblocks towards realizing the promise envisioned by 90-90-90. For instance, ensuring that 90% of HIV-infected persons know their status will require a large increase in access to HIV tests compared with what is currently available. To ensure that there are enough anti-retrovirals available to treat the nearly 25 million people that will require them by 2020 represents a near doubling of the ARV supplied to treat the 13 million currently on treatment. Similarly, to monitor those on treatment means an unprecedented scale-up of viral load testing throughout Africa. Conclusions Larger issues include whether the capacity exists at the local level to handle these commodities when they arrive in the most severely affected countries, including considerations of the human resources and costs needed to make this strategy effective. We believe that such “real world” analysis of proposed strategies and policies is essential to ensure their most effective implementation. PMID:27370169

  1. The 90 90 90 strategy to end the HIV Pandemic by 2030: Can the supply chain handle it?

    Science.gov (United States)

    Jamieson, David; Kellerman, Scott E

    2016-01-01

    UNAIDS "90-90-90" strategy calls for 90% of HIV-infected individuals to be diagnosed by 2020, 90% of whom will be on anti-retroviral therapy (ART) and 90% of whom will achieve sustained virologic suppression. Reaching these targets by 2020 will reduce the HIV epidemic to a low-level endemic disease by 2030. However, moving the global response towards this universal test and treat model will pose huge challenges to public health systems in resource-limited settings, including global and local supply chain systems. These challenges are especially acute in Africa, which accounts for over 70% of the persons affected by HIV. From a supply chain perspective, each of the "90's" has possible complications and roadblocks towards realizing the promise envisioned by 90-90-90. For instance, ensuring that 90% of HIV-infected persons know their status will require a large increase in access to HIV tests compared with what is currently available. To ensure that there are enough anti-retrovirals available to treat the nearly 25 million people that will require them by 2020 represents a near doubling of the ARV supplied to treat the 13 million currently on treatment. Similarly, to monitor those on treatment means an unprecedented scale-up of viral load testing throughout Africa. Larger issues include whether the capacity exists at the local level to handle these commodities when they arrive in the most severely affected countries, including considerations of the human resources and costs needed to make this strategy effective. We believe that such "real world" analysis of proposed strategies and policies is essential to ensure their most effective implementation.

  2. Validation of Extraction Paper Chromatography as a Quality Control Technique for Analysis of Sr-90 in Y-90 Product

    International Nuclear Information System (INIS)

    Nipavan, Poramatikul; Jatupol, Sangsuriyan; Wiranee, Sriweing

    2009-07-01

    Full text: Yttrium-90 (Y-90) is a daughter product of strontium-90 (Sr-90). It is specified that there should be less than 2 micro-curie of Sr-90 in Y-90 radiopharmaceuticals. Since both nuclides are beta emitting and there is always a contamination of Y-90 in Sr-90 sample, validation of the analytical method is necessary. In this study, commercial Y-90 and Sr-85 (a gamma emitting isotope of strontium) were used as daughter and mother nuclides, respectively. Extraction paper chromatography technique and its efficient validation method were investigated. Bis-(2-ethylhexyl) diphosphonate was dropped at the origin of chromatography paper and air dried prior to sample drops. Validation of the separation was done by radio-chromatography scanning of the chromatography paper. Their energy spectra were identified in the spectra mode of Packard Cobra II automatic gamma counter, which can differentiate a pure gamma, a pure beta and a mixture of beta and gamma nuclides. Results showed that yttrium acetate remained fixed at the origin of the chromatography paper while strontium acetate moved to the solvent front when developed in saline. In conclusion, the extraction paper chromatography technique can effectively separate Sr-90 from Y-90

  3. Absence of diabetes and pancreatic exocrine dysfunction in a transgenic model of carboxyl-ester lipase-MODY (maturity-onset diabetes of the young.

    Directory of Open Access Journals (Sweden)

    Helge Ræder

    Full Text Available CEL-MODY is a monogenic form of diabetes with exocrine pancreatic insufficiency caused by mutations in CARBOXYL-ESTER LIPASE (CEL. The pathogenic processes underlying CEL-MODY are poorly understood, and the global knockout mouse model of the CEL gene (CELKO did not recapitulate the disease. We therefore aimed to create and phenotype a mouse model specifically over-expressing mutated CEL in the pancreas.We established a monotransgenic floxed (flanking LOX sequences mouse line carrying the human CEL mutation c.1686delT and crossed it with an elastase-Cre mouse to derive a bitransgenic mouse line with pancreas-specific over-expression of CEL carrying this disease-associated mutation (TgCEL. Following confirmation of murine pancreatic expression of the human transgene by real-time quantitative PCR, we phenotyped the mouse model fed a normal chow and compared it with mice fed a 60% high fat diet (HFD as well as the effects of short-term and long-term cerulein exposure.Pancreatic exocrine function was normal in TgCEL mice on normal chow as assessed by serum lipid and lipid-soluble vitamin levels, fecal elastase and fecal fat absorption, and the normoglycemic mice exhibited normal pancreatic morphology. On 60% HFD, the mice gained weight to the same extent as controls, had normal pancreatic exocrine function and comparable glucose tolerance even after resuming normal diet and follow up up to 22 months of age. The cerulein-exposed TgCEL mice gained weight and remained glucose tolerant, and there were no detectable mutation-specific differences in serum amylase, islet hormones or the extent of pancreatic tissue inflammation.In this murine model of human CEL-MODY diabetes, we did not detect mutation-specific endocrine or exocrine pancreatic phenotypes, in response to altered diets or exposure to cerulein.

  4. Absence of diabetes and pancreatic exocrine dysfunction in a transgenic model of carboxyl-ester lipase-MODY (maturity-onset diabetes of the young).

    Science.gov (United States)

    Ræder, Helge; Vesterhus, Mette; El Ouaamari, Abdelfattah; Paulo, Joao A; McAllister, Fiona E; Liew, Chong Wee; Hu, Jiang; Kawamori, Dan; Molven, Anders; Gygi, Steven P; Njølstad, Pål R; Kahn, C Ronald; Kulkarni, Rohit N

    2013-01-01

    CEL-MODY is a monogenic form of diabetes with exocrine pancreatic insufficiency caused by mutations in CARBOXYL-ESTER LIPASE (CEL). The pathogenic processes underlying CEL-MODY are poorly understood, and the global knockout mouse model of the CEL gene (CELKO) did not recapitulate the disease. We therefore aimed to create and phenotype a mouse model specifically over-expressing mutated CEL in the pancreas. We established a monotransgenic floxed (flanking LOX sequences) mouse line carrying the human CEL mutation c.1686delT and crossed it with an elastase-Cre mouse to derive a bitransgenic mouse line with pancreas-specific over-expression of CEL carrying this disease-associated mutation (TgCEL). Following confirmation of murine pancreatic expression of the human transgene by real-time quantitative PCR, we phenotyped the mouse model fed a normal chow and compared it with mice fed a 60% high fat diet (HFD) as well as the effects of short-term and long-term cerulein exposure. Pancreatic exocrine function was normal in TgCEL mice on normal chow as assessed by serum lipid and lipid-soluble vitamin levels, fecal elastase and fecal fat absorption, and the normoglycemic mice exhibited normal pancreatic morphology. On 60% HFD, the mice gained weight to the same extent as controls, had normal pancreatic exocrine function and comparable glucose tolerance even after resuming normal diet and follow up up to 22 months of age. The cerulein-exposed TgCEL mice gained weight and remained glucose tolerant, and there were no detectable mutation-specific differences in serum amylase, islet hormones or the extent of pancreatic tissue inflammation. In this murine model of human CEL-MODY diabetes, we did not detect mutation-specific endocrine or exocrine pancreatic phenotypes, in response to altered diets or exposure to cerulein.

  5. Development of methodology for the preparation of 90Sr-90Y generators

    International Nuclear Information System (INIS)

    Barrio, Graciela; Osso, Joao A.

    2008-01-01

    Among the more attractive radionuclides for therapeutic applications is yttrium-90. Its relatively short half-life (64.0h), maximum beta energy (2.28 MeV) make it well suited for a variety of applications, ranging from the radiolabeling of antibodies for tumor therapy to the production of radiolabeled particles for the treatment of liver malignancies. Yttrium-90 results from the decay of strontium-90 (28y) and decays to a stable daughter ( 90 Zr) and has no γ-ray, what makes this nuclide very attractive for therapeutic applications. 90 Y is produced through a generator system, from the decay of 90 Sr. Before it can safely employed in clinical applications, the 90 Y must be made essentially free of 90 Sr, an isotope known to cause bone marrow suppression. The objective of this work is to develop 90 Sr- 90 Y generator using a cation exchange method resin. This method was the most effects for the separation of 90 Y and 90 Sr. 90 Sr is strongly adsorbed in the resin and 90 Y is eluted in 0.003M EDTA. The generator efficiency and radionuclide control results were also evaluated using 85 Sr (γ-ray emitter). The generator was developed, using AG 50WX8 exchange resin converted to the Na+ form and then conditioned with 0.003M EDTA solution (pH=4.8). The quality control showed that 90 Y had no 8 '5Sr impurities, inside the detection limits of the detector. The results presented here are very promising, showing that the methodology is able to separate the two nuclides, with low quantities of 90 Sr impurity and satisfactory elution yields. (author)

  6. Plasma and blood volume in the calf from birth till 90 days of age

    International Nuclear Information System (INIS)

    Moellerberg, L.; Ekman, L.; Jacobsson, S.-O.

    1975-01-01

    Determinations of plasma volume were made of 9 clinically healthy Swedish Red and White calves from birth to 90 days of age by means of the isotop dilution technique. Commercially available 131 I labelled human serum albumin was used. Calculation of the total blood volume was based on the plasma volume and packed cell volume. The plasma and blood volumes increased per kg body weight in average 17 and 14 percent respectively from directly after birth to 24 hrs. old. From 1 to 90 days of age the plasma and blood volume fell steadily per kg body weight. Plasma volume expressed as a percentage of body weight was 5.3 percent at birth, 6.5 percent at 1 day old, and 4.9 percent at 90 days old. Corresponding values for blood were 8.4, 9.3 and 7.0 percent. (author)

  7. Unplanned readmission within 90 days after pediatric neurosurgery.

    Science.gov (United States)

    Chotai, Silky; Guidry, Bradley S; Chan, Emily W; Sborov, Katherine D; Gannon, Stephen; Shannon, Chevis; Bonfield, Christopher M; Wellons, John C; Naftel, Robert P

    2017-12-01

    OBJECTIVE Readmission and return to operating room after surgery are increasingly being used as a proxy for quality of care. Nearly 60% of these readmissions are unplanned, which translates into billions of dollars in health care costs. The authors set out to analyze the incidence of readmission at their center, to define causes of unplanned readmission, and to determine the preoperative and surgical variables associated with readmissions following pediatric neurosurgery. METHODS A total of 536 children who underwent operations for neurosurgical diagnoses between 2012 and 2015 and who were later readmitted were included in the final analysis. Unplanned readmissions were defined to have occurred as a result of complications within 90 days after index surgery. Patient records were retrospectively reviewed to determine the primary diagnosis, surgery indication, and cause of readmission and return to operating room. The cost for index hospitalization, readmission episode, and total cost were derived based on the charges obtained from administrative data. Bivariate and multivariable analyses were conducted. RESULTS Of 536 patients readmitted in total, 17.9% (n = 96) were readmitted within 90 days. Of the overall readmissions, 11.9% (n = 64) were readmitted within 30 days, and 5.97% (n = 32) were readmitted between 31 and 90 days. The median duration between discharge and readmission was 20 days (first quartile [Q1]: 9 days, third quartile [Q3]: 36 days). The most common reason for readmission was shunt related (8.2%, n = 44), followed by wound infection (4.7%, n = 25). In the risk-adjusted multivariable logistic regression model for total 90-day readmission, patients with the following characteristics: younger age (p = 0.001, OR 0.886, 95% CI 0.824-0.952); "other" (nonwhite, nonblack) race (p = 0.024, OR 5.49, 95% CI 1.246-24.2); and those born preterm (p = 0.032, OR 2.1, 95% CI 1.1-4.12) had higher odds of being readmitted within 90 days after discharge. The total

  8. Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance.

    Science.gov (United States)

    Kern, Marcelo; McGeehan, John E; Streeter, Simon D; Martin, Richard N A; Besser, Katrin; Elias, Luisa; Eborall, Will; Malyon, Graham P; Payne, Christina M; Himmel, Michael E; Schnorr, Kirk; Beckham, Gregg T; Cragg, Simon M; Bruce, Neil C; McQueen-Mason, Simon J

    2013-06-18

    Nature uses a diversity of glycoside hydrolase (GH) enzymes to convert polysaccharides to sugars. As lignocellulosic biomass deconstruction for biofuel production remains costly, natural GH diversity offers a starting point for developing industrial enzymes, and fungal GH family 7 (GH7) cellobiohydrolases, in particular, provide significant hydrolytic potential in industrial mixtures. Recently, GH7 enzymes have been found in other kingdoms of life besides fungi, including in animals and protists. Here, we describe the in vivo spatial expression distribution, properties, and structure of a unique endogenous GH7 cellulase from an animal, the marine wood borer Limnoria quadripunctata (LqCel7B). RT-quantitative PCR and Western blot studies show that LqCel7B is expressed in the hepatopancreas and secreted into the gut for wood degradation. We produced recombinant LqCel7B, with which we demonstrate that LqCel7B is a cellobiohydrolase and obtained four high-resolution crystal structures. Based on a crystallographic and computational comparison of LqCel7B to the well-characterized Hypocrea jecorina GH7 cellobiohydrolase, LqCel7B exhibits an extended substrate-binding motif at the tunnel entrance, which may aid in substrate acquisition and processivity. Interestingly, LqCel7B exhibits striking surface charges relative to fungal GH7 enzymes, which likely results from evolution in marine environments. We demonstrate that LqCel7B stability and activity remain unchanged, or increase at high salt concentration, and that the L. quadripunctata GH mixture generally contains cellulolytic enzymes with highly acidic surface charge compared with enzymes derived from terrestrial microbes. Overall, this study suggests that marine cellulases offer significant potential for utilization in high-solids industrial biomass conversion processes.

  9. Validation of an extraction paper chromatography (EPC) technique for estimation of trace levels of 90Sr in 90Y solutions obtained from 90Sr/90Y generator systems

    International Nuclear Information System (INIS)

    Usha Pandey; Yogendra Kumar; Ashutosh Dash

    2014-01-01

    While the extraction paper chromatography (EPC) technique constitutes a novel paradigm for the determination of few Becquerels of 90 Sr in MBq quantities of 90 Y obtained from 90 Sr/ 90 Y generator, validation of the technique is essential to ensure its usefulness as a real time analytical tool. With a view to explore the relevance and applicability of EPC technique as a real time quality control (QC) technique for the routine estimation of 90 Sr content in generator produced 90 Y, a systematic validation study was carried out diligently not only to establish its worthiness but also to broaden its horizon. The ability of the EPC technique to separate trace amounts of Sr 2+ in the presence of large amounts of Y 3+ was verified. The specificity of the technique for Y 3+ was demonstrated with 90 Y obtained by neutron irradiation. The method was validated under real experimental conditions and compared with a QC method described in US Pharmacopeia for detection of 90 Sr levels in 90 Y radiopharmaceuticals. (author)

  10. Long-term change of site response after the M W 9.0 Tohoku earthquake in Japan

    Science.gov (United States)

    Wu, Chunquan; Peng, Zhigang

    2012-12-01

    The recent M W 9.0 off the Pacific coast of Tohoku earthquake is the largest recorded earthquake in Japan's history. The Tohoku main shock and its aftershocks generated widespread strong shakings as large as ~3000 Gal along the east coast of Japan. Wu and Peng (2011) found clear drop of resonant frequency of up to 70% during the Tohoku main shock at 6 sites and correlation of resonance (peak) frequency and peak ground acceleration (PGA) during the main shock. Here we follow that study and systematically analyze long-term changes of material properties in the shallow crust from one year before to 5 months after the Tohoku main shock, using seismic data recorded by the Japanese Strong Motion Network KiK-Net. We use sliding window spectral ratios computed from a pair of surface and borehole stations to track the temporal changes in the site response of 6 sites. Our results show two stages of logarithmic recovery after a sharp drop of resonance frequency during the Tohoku main shock. The first stage is a rapid recovery within several hundred seconds to several hours, and the second stage is a slow recovery of more than five months. We also investigate whether the damage caused by the Tohoku main shock could make the near surface layers more susceptible to further damages, but we do not observe clear changes in susceptibility to further damage before and after the Tohoku main shock.

  11. Application of a net-based baseline correction scheme to strong-motion records of the 2011 Mw 9.0 Tohoku earthquake

    Science.gov (United States)

    Tu, Rui; Wang, Rongjiang; Zhang, Yong; Walter, Thomas R.

    2014-06-01

    The description of static displacements associated with earthquakes is traditionally achieved using GPS, EDM or InSAR data. In addition, displacement histories can be derived from strong-motion records, allowing an improvement of geodetic networks at a high sampling rate and a better physical understanding of earthquake processes. Strong-motion records require a correction procedure appropriate for baseline shifts that may be caused by rotational motion, tilting and other instrumental effects. Common methods use an empirical bilinear correction on the velocity seismograms integrated from the strong-motion records. In this study, we overcome the weaknesses of an empirically based bilinear baseline correction scheme by using a net-based criterion to select the timing parameters. This idea is based on the physical principle that low-frequency seismic waveforms at neighbouring stations are coherent if the interstation distance is much smaller than the distance to the seismic source. For a dense strong-motion network, it is plausible to select the timing parameters so that the correlation coefficient between the velocity seismograms of two neighbouring stations is maximized after the baseline correction. We applied this new concept to the KiK-Net and K-Net strong-motion data available for the 2011 Mw 9.0 Tohoku earthquake. We compared the derived coseismic static displacement with high-quality GPS data, and with the results obtained using empirical methods. The results show that the proposed net-based approach is feasible and more robust than the individual empirical approaches. The outliers caused by unknown problems in the measurement system can be easily detected and quantified.

  12. Actualizing of calibration curves of "1"4C/C, "9"0Sr/Ca, "2"2"8Th/"2"3"2Th in ivory for the determination of the post mortal interval of elephants and consequences of the radiation protection of non-human species

    International Nuclear Information System (INIS)

    Schupfner, R.

    2016-01-01

    The determination of the activity concentration of the radionuclides "1"4C/C and "9"0Sr/Ca and "2"2"8Th/"2"3"2Th applying combined radionuclide analyses methods has been proved to be a suitable tool for the purpose of an unambiguous age determination of elephant ivory [1, 2, 3, 10, 11, 12, 13]. Analysing representative and independently dated samples (N = 28) of ivory the curves fitting the post mortal interval (PMI) versus the activity concentration of the radionuclides mentioned above produced the data base enabling a more unambiguous age determination. Data from these studies origin [1, 2, 3, 10, 11, 12, 13] in analyses of ivory samples which were available up to the 2012. During the last five years there was a gap in information of the future trend of "1"4C/C and "9"0Sr/Ca. Up to this study it was not possible to assess whether the future level of "1"4C/C as well as "9"0Sr/Ca can analytically be distinguished from the level before 1954. At about 1954 the activity concentration of radionuclides from the atmospheric nuclear explosion, as "1"4C and "9"0Sr, increased in ivory significantly. This study aims in closing this information gap. The results of analyses of "1"4C/C, "9"0Sr/Ca, "2"2"8Th/"2"3"2Th in ivory with PMI values ranging from 1 to 5 years are presented and interpreted. These data enable an actualization of the calibration curves of PMI versus specific activities. This is necessary for a better understanding of the effect of blindness of "1"4C/C dating and its prevention. On the base of all available results form independent dated ivory sample available up to 2015 a suitable analytical procedure is suggested which aims in a more precise and reliable age determination of elephant tusks. Results of determining of radionuclides "1"4C/C and "9"0Sr/Ca and "2"2"8Th/"2"3"2Th in ivory are shown from before 1950 to 2015. These results are discussed with respect the purposes of dating as well as their significance to the radiation protection of nonhuman species.

  13. Determination of 90Sr Abundance in Different Kind of Pasture at PPTN Serpong

    International Nuclear Information System (INIS)

    Sudiyati; Murdahayu Makmur; Agus Gindo S

    2007-01-01

    The determination of 90 Sr abundance in the pasture grass around Serpong had been done. The aim of the research is to determine 90 Sr abundance in the pasture which could be used to estimate the dose received by human in food chain pathways. The mean of 90 Sr mean activities in pasture around PPTN Serpong is 14.14 ± 3.7 mBq/kg, this result is insignificantly different compared to its control (off-site), i.e. 14.18 ± 6.9 mBq/kg. Elephant grass (Penisettum Purpureum) has the highest abundance of 90 Sr which is 24.8 ± 15.6 mBq/kg, followed by teki grass (Setaria Spha Celata) and ilalang grass (Imperata Cylinrica) which are 14.9 ± 6.0 mBq/kg and 8.0 ± 4.8 mBq/kg, respectively. (author)

  14. Inventory and vertical migration of {sup 90}Sr fallout and {sup 137}Cs/{sup 90}Sr ratio in Spanish mainland soils

    Energy Technology Data Exchange (ETDEWEB)

    Herranz, M. [Department Nuclear Engineering and Fluid Mechanics, University of the Basque, Country (UPV/EHU), Alda Urquijo s/n, E-48013 Bilbao (Spain); Romero, L.M. [CIEMAT, Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas, Av. Complutense 22, 28040 Madrid (Spain); Idoeta, R. [Department Nuclear Engineering and Fluid Mechanics, University of the Basque, Country (UPV/EHU), Alda Urquijo s/n, E-48013 Bilbao (Spain); Olondo, C., E-mail: kontxi.olondo@ehu.es [Department Nuclear Engineering and Fluid Mechanics, University of the Basque, Country (UPV/EHU), Alda Urquijo s/n, E-48013 Bilbao (Spain); Valino, F. [CIEMAT, Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas, Av. Complutense 22, 28040 Madrid (Spain); Legarda, F. [Department Nuclear Engineering and Fluid Mechanics, University of the Basque, Country (UPV/EHU), Alda Urquijo s/n, E-48013 Bilbao (Spain)

    2011-11-15

    In this paper the inventory of {sup 90}Sr in 34 points distributed along the Spanish peninsular territory is presented. Obtained values range between 173 Bq/m{sup 2} and 2047 Bq/m{sup 2}. From these data set and those {sup 137}Cs data obtained in a previous work the {sup 137}Cs/{sup 90}Sr activity ratio has been established, laying this value between 0.9 and 3.6. Also the migration depth of both radionuclides has been analysed obtaining for {sup 137}Cs an average value 57% lower than that obtained for {sup 90}Sr. Additionally, this paper presents the results obtained in 11 sampling points in which the activity vertical profile has been measured. These profiles have been analysed to state the behaviour of strontium in soils and after, by using a convective-diffusive model, the parameters of the model which governs the vertical migration of {sup 90}Sr in the soil, v (apparent convection velocity) and D (apparent diffusion coefficient) have been evaluated. Mean values obtained are 0.20 cm/year and 3.67 cm{sup 2}/year, respectively. - Highlights: > Measured {sup 90}Sr activity in Spanish mainland, being within a range of [173, 2047] Bq/m{sup 2}, with a mean value of 793 Bq/m{sup 2}. > Compared the migration capacity of {sup 137}Cs and {sup 90}Sr in the same soils. > {sup 90}Sr shows a unique behaviour tendency in soil. > The parameters which govern the applied model have been obtained for the analysed profiles. > Analysed those parameters' values also reflects this unique tendency.

  15. Monograph of Diplachne (Poaceae, Chloridoideae, Cynodonteae).

    Science.gov (United States)

    Snow, Neil; Peterson, Paul M; Romaschenko, Konstantin; Simon, Bryan K

    2018-01-01

    Diplachne P. Beauv. comprises two species with C 4 (NAD-ME) photosynthesis. Diplachne fusca has a nearly pantropical-pantemperate distribution with four subspecies: D. fusca subsp. fusca is Paleotropical with native distributions in Africa, southern Asia and Australia; the widespread Australian endemic D. f. subsp. muelleri; and D. f. subsp. fascicularis and D. f. subsp. uninervia occurring in the New World. Diplachne gigantea is known from a few widely scattered, older collections in east-central and southern Africa, and although Data Deficient clearly is of conservation concern. A discussion of previous taxonomic treatments is provided, including molecular data supporting Diplachne in its newer, restricted sense. Many populations of Diplachne fusca are highly tolerant of saline substrates and most prefer seasonally moist to saturated soils, often in disturbed areas. Some populations of Diplachne fusca in southern Asia combine nitrogen-fixation, high salinity tolerance and palatibilty to livestock, which should be pursued with further research for purposes of soil reclamation. Diplachne fusca subsp. uninervia is the most invasive of the subspecies and is becoming weedy in some non-native areas, including in the Old World. This monograph provides detailed descriptions of all taxa, a key to the species and subspecies, geographic distributions and information on the anatomy of leaves, stems, lemmatal micromorphology and discussions of the chromosome numbers. Lectotypes are designated for: Atropis carinata Grisb.; Diplachne acuminata Nash; Diplachne capensis (Nees) Nees var. concinna Nees; Diplachne capensis (Nees) Nees var. obscura Nees, Diplachne capensis (Nees) Nees var. prolifera subvar. minor Nees, Diplachne halei Nash, Diplachne maritima E.P. Bicknel, Diplachne muelleri Benth., Diplachne reverchonii Vasey, Diplachne tectoneticola Backer, Leptochloa imbricata Thurb., Leptochloa neuroglossa Peter, Leptochloa uninervia var. typica fo. abbreviata Parodi, Triodia

  16. When patients fail UNAIDS' last 90 - the "failure cascade" beyond 90-90-90 in rural Lesotho, Southern Africa: a prospective cohort study.

    Science.gov (United States)

    Labhardt, Niklaus Daniel; Ringera, Isaac; Lejone, Thabo Ishmael; Cheleboi, Molisana; Wagner, Sarah; Muhairwe, Josephine; Klimkait, Thomas

    2017-07-19

    HIV-infected individuals on first-line antiretroviral therapy (ART) in resource-limited settings who do not achieve the last "90" (viral suppression) enter a complex care cascade: enhanced adherence counselling (EAC), repetition of viral load (VL) and switch to second-line ART aiming to achieve resuppression. This study describes the "failure cascade" in patients in Lesotho. Patients aged ≥16 years on first-line ART at 10 facilities in rural Lesotho received a first-time VL in June 2014. Those with VL ≥80 copies/mL were included in a cohort. The care cascade was assessed at four points: attendance of EAC, result of follow-up VL after EAC, switch to second-line in case of sustained unsuppressed VL and outcome 18 months after the initial unsuppressed VL. Multivariate logistic regression was used to assess predictors of being retained in care with viral resuppression at follow-up. Out of 1563 patients who underwent first-time VL, 138 (8.8%) had unsuppressed VL in June 2014. Out of these, 124 (90%) attended EAC and 116 (84%) had follow-up VL (4 died, 2 transferred out, 11 lost, 5 switched to second-line before follow-up VL). Among the 116 with follow-up VL, 36 (31%) achieved resuppression. Out of the 80 with sustained unsuppressed VL, 58 were switched to second-line, the remaining continued first line. At 18 months' follow-up in December 2015, out of the initially 138 with unsuppressed VL, 56 (41%) were in care and virally suppressed, 37 (27%) were in care with unsuppressed VL and the remaining 45 (33%) were lost, dead, transferred to another clinic or without documented VL. Achieving viral resuppression after EAC (adjusted odds ratio (aOR): 5.02; 95% confidence interval: 1.14-22.09; p  = 0.033) and being switched to second-line in case of sustained viremia after EAC (aOR: 7.17; 1.90-27.04; p  = 0.004) were associated with being retained in care and virally suppressed at 18 months of follow-up. Age, gender, education, time on ART and level of VL were not

  17. How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?

    Science.gov (United States)

    Yoav, Shahar; Barak, Yoav; Shamshoum, Melina; Borovok, Ilya; Lamed, Raphael; Dassa, Bareket; Hadar, Yitzhak; Morag, Ely; Bayer, Edward A

    2017-01-01

    Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic cocktails is critical for increasing the cost-effectiveness of bioethanol production. In this context, the multi-protein cellulolytic complex of Clostridium ( Ruminiclostridium ) thermocellum, the cellulosome, was studied here. C. thermocellum is known to assemble cellulosomes of various subunit (enzyme) compositions, in response to the available carbon source. In the current study, different carbon sources were used, and their influence on both cellulosomal composition and the resultant activity was investigated. Glucose, cellobiose, microcrystalline cellulose, alkaline-pretreated switchgrass, alkaline-pretreated corn stover, and dilute acid-pretreated corn stover were used as sole carbon sources in the growth media of C. thermocellum strain DSM 1313. The purified cellulosomes were compared for their activity on selected cellulosic substrates. Interestingly, cellulosomes derived from cells grown on lignocellulosic biomass showed no advantage in hydrolyzing the original carbon source used for their production. Instead, microcrystalline cellulose- and glucose-derived cellulosomes were equal or superior in their capacity to deconstruct lignocellulosic biomass. Mass spectrometry analysis revealed differential composition of catalytic and structural subunits (scaffoldins) in the different cellulosome samples. The most abundant catalytic subunits in all cellulosome types include Cel48S, Cel9K, Cel9Q, Cel9R, and Cel5G. Microcrystalline cellulose- and glucose-derived cellulosome samples showed higher endoglucanase-to-exoglucanase ratios and higher catalytic subunit-per-scaffoldin ratios compared to lignocellulose-derived cellulosome types. The results reported here highlight the finding that cellulosomes derived from cells grown on glucose

  18. Determination of 90Sr in crude coconut oil by Cherenkov radiation measurement

    International Nuclear Information System (INIS)

    Santos, F.L.; Esguerra, L.V.; Castaneda, S.S.

    1991-01-01

    In December 1989, the Analytical Measurements Research group received crude coconut oil samples for radiometric determination of 90 Sr as required by the government of an importing country. The limit for 90 Sr in imported products was 67 BqKg -1 . The possibility of 90 Sr contamination of coconut oil is remote, considering that the molecular structures of its constituents do not include inorganic species. However, a method still had to be developed for certifying to the absence of 90 Sr in the samples. The technique selected was based on the direct measurement of 90 Sr/ 90 Y Cherenkov radiation in oil. For 10 ml of samples (approximately 9 g.) and a counting period of 1 hr. lower limits of detection ranging from 16 to 27 BqKg -1 were obtained. These detection limits, being well below the regulatory limit of 67 BqKg -1 for 90 Sr were adequate for purposes of the study. A total of ten crude coconut oil samples analyzed between December 1989 and June 1990 did not have detectable 90 Sr/ 90 Y activities. (auth.). 7 refs.; 1 fig

  19. Regularity on absorption and accumulation of 90Sr in yellow-feather broiler (Hypeco)

    International Nuclear Information System (INIS)

    Xu Shiming; Hou Lanxin; Zhao Wenhu; Li Xia; Yang Zhongyuan

    1996-02-01

    The feeding experiment was carried for yellow-feather broiler (Hypeco) with 90 Sr[NO 3 ] 2 having of 1.85 x 10 2 ∼1.85 x 10 5 Bq/chicken to observe the regular pattern of absorption and accumulation of 90 Sr in its organs and tissues. The absorption rate of 90 Sr by oral feeding is very low. The most of 90 Sr is drained away by daily excreta from the body. The half-time of its feeding is within 24 hours. The more 90 Sr is fed, the more 90 Sr is absorbed, however, the uptake rate of 90 Sr is decreasing. The affinity of 90 Sr for bone is very strong and the partition ratio is about 90%∼99%. The absorption coefficient of 90 Sr in organs and tissues of broiler is presented. (2 refs., 9 tabs.)

  20. The 250AH/90A active lithium-thionyl chloride cell for Centaur-G application

    Science.gov (United States)

    Zolla, A. E.; Tura, D. D.

    1987-09-01

    A high rate active Li/SOCl2 cell was designed for use in a 28 volt, 250 amp-hour space battery system. The lithium battery is being considered as a replacement of its heavier silver-zinc counterpart on board the Centaur-G booster rocket which is used to launch payloads from the Space Shuttle cargo bay into deep-space. Basically a feasibility study, this development effort is demonstrating the ability of the lithium cell to deliver up to 90 amps safely at power densities of approximately 25 watts per pound. Test data on 4 prototype units is showing an energy density of 85 watt-hours per pound and 9.0 watt-hours/cu in. The cells tested typically delivered 280 to 300 amp-hours under ambient temperature test conditions using alternating continuous loads of 90, 55, and 20 amperes throughout life. Data from four cells tested are presented to demonstrate the capability of Li/SOCl2 technology for a C/3 discharge rate in active and hermetic cell units.

  1. Utilization of a novel electrochemical {sup 90}Sr/{sup 90}Y generator for the preparation of {sup 90}Y-labeled RGD peptide dimer in clinically relevant dose

    Energy Technology Data Exchange (ETDEWEB)

    Chakraborty, Sudipta; Chakravarty, Rubel; Pillai, Maroor Raghavan Ambikalmajan; Dash, Ashutosh [Bhabha Atomic Research Centre, Mumbai (India). Radiopharmaceuticals Div.; Sarma, Haladhar Dev [Bhabha Atomic Research Centre, Mumbai (India). Radiation Biology and Health Sciences Div.

    2014-09-01

    The work reported in this paper provides a systematic study towards the development of an optimized strategy for preparation of a clinically relevant dose of {sup 90}Y-labeled dimeric RGD peptide derivative, DOTA-E[c(RGDfK)]{sub 2} [DOTA-(RGD){sub 2}] for in vivo targeted therapy utilizing {sup 90}Y obtained from a novel electrochemical {sup 90}Sr/{sup 90}Y generator. The performance of the generator was evaluated to ensure its suitability for providing {sup 90}Y in adequate quantity and purity required for formulation of clinically relevant dose for PRRT. {sup 90}Y-DOTA-(RGD){sub 2} was synthesized in high yield (86.2 ± 2.5%) and radiochemical purity (98.4 ± 0.5%) using clinically relevant dose (∝ 3.8 GBq) of {sup 90}Y. In vitro stability studies revealed that the radiolabeled conjugate retained its radiochemical purity in normal saline and human serum. Preliminary biodistribution studies carried out in C57/BL6 mice bearing melanoma tumors showed that the preparation exhibited significant tumor uptake (5.30 ± 0.78% of injected activity at 30 min post-injection) with good tumor to background ratio. The optimized radiolabeling protocol seems to be an attractive strategy which is largely viewed as a springboard to realize scope of developing {sup 90}Y labeled cyclic RGD peptides for targeted therapy of tumors over-expressing integrin-α{sub ν}β{sub 3} receptors. (orig.)

  2. ANSI/ASHRAE/IESNA Standard 90.1-2007 Final Determination Quantitative Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Halverson, Mark A.; Liu, Bing; Richman, Eric E.; Winiarski, David W.

    2011-05-01

    The United States (U.S.) Department of Energy (DOE) conducted a final quantitative analysis to assess whether buildings constructed according to the requirements of the American National Standards Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE)/Illuminating Engineering Society of North America (IESNA) Standard 90.1-2007 would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IESNA Standard 90.1-2004. The final analysis considered each of the 44 addenda to ANSI/ASHRAE/IESNA Standard 90.1-2004 that were included in ANSI/ASHRAE/IESNA Standard 90.1-2007. All 44 addenda processed by ASHRAE in the creation of Standard 90.1-2007 from Standard 90.1-2004 were reviewed by DOE, and their combined impact on a suite of 15 building prototype models in 15 ASHRAE climate zones was considered. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE’s final determination. However, out of the 44 addenda, 9 were preliminarily determined to have measureable and quantifiable impact.

  3. Cohort study of somatostatin-based radiopeptide therapy with [(90)Y-DOTA]-TOC versus [(90)Y-DOTA]-TOC plus [(177)Lu-DOTA]-TOC in neuroendocrine cancers.

    Science.gov (United States)

    Villard, Linda; Romer, Anna; Marincek, Nicolas; Brunner, Philippe; Koller, Michael T; Schindler, Christian; Ng, Quinn K T; Mäcke, Helmut R; Müller-Brand, Jan; Rochlitz, Christoph; Briel, Matthias; Walter, Martin A

    2012-04-01

    Radiopeptide therapy is commonly performed with a single radioisotope. We aimed to compare the effectiveness of somatostatin-based radiopeptide therapy with a single versus a combination of radioisotopes. In a cohort study, patients with metastasized neuroendocrine cancer were treated with repeated cycles of (90)yttrium-labeled tetraazacyclododecane-tetraacetic acid modified Tyr-octreotide ([(90)Y-DOTA]-TOC) or with cycles alternating between [(90)Y-DOTA]-TOC and (177)lutetium-labeled DOTA-TOC ([(177)Lu-DOTA]-TOC) until tumor progression or permanent toxicity. Multivariable Cox regression and competing risk regression were used to study predictors of survival and renal toxicity in patients completing three or more treatment cycles. A total of 486 patients completed three or more treatment cycles; 237 patients received [(90)Y-DOTA]-TOC and 249 patients received [(90)Y-DOTA]-TOC + [(177)Lu-DOTA]-TOC. Patients receiving [(90)Y-DOTA]-TOC + [(177)Lu-DOTA]-TOC had a significantly longer survival than patients receiving [(90)Y-DOTA]-TOC alone (5.51 v 3.96 years; hazard ratio, 0.64; 95% CI, 0.47 to 0.88; P = .006). The rates of severe hematologic toxicities (6.3% v 4.4%; P = .25) and severe renal toxicity (8.9% v 11.2%; P = .47) were comparable in both groups. [(90)Y-DOTA]-TOC + [(177)Lu-DOTA]-TOC was associated with improved overall survival compared with [(90)Y-DOTA]-TOC alone in patients completing three or more cycles of treatment. Contrary to the current practice in radiopeptide therapy, our results suggest an advantage of using a combination of radioisotopes.

  4. Heat Shock Protein 90 (Hsp90 Expression and Breast Cancer

    Directory of Open Access Journals (Sweden)

    Christos A. Papadimitriou

    2012-09-01

    Full Text Available Hsp90 is an abundant protein in mammalian cells. It forms several discrete complexes, each containing distinct groups of co-chaperones that assist protein folding and refolding during stress, protein transport and degradation. It interacts with a variety of proteins that play key roles in breast neoplasia including estrogen receptors, tumor suppressor p53 protein, angiogenesis transcription factor HIF-1alpha, antiapoptotic kinase Akt, Raf-1 MAP kinase and a variety of receptor tyrosine kinases of the erbB family. Elevated Hsp90 expression has been documented in breast ductal carcinomas contributing to the proliferative activity of breast cancer cells; whilst a significantly decreased Hsp90 expression has been shown in infiltrative lobular carcinomas and lobular neoplasia. Hsp90 overexpression has been proposed as a component of a mechanism through which breast cancer cells become resistant to various stress stimuli. Therefore, pharmacological inhibition of HSPs can provide therapeutic opportunities in the field of cancer treatment. 17-allylamino,17-demethoxygeldanamycin is the first Hsp90 inhibitor that has clinically been investigated in phase II trial, yielding promising results in patients with HER2-overexpressing metastatic breast cancer, whilst other Hsp90 inhibitors (retaspimycin HCL, NVP-AUY922, NVP-BEP800, CNF2024/BIIB021, SNX-5422, STA-9090, etc. are currently under evaluation.

  5. Marked hybridization and introgression in Ophrys sect. Pseudophrys in the western Iberian Peninsula.

    Science.gov (United States)

    Cotrim, Helena; Monteiro, Filipa; Sousa, Eva; Pinto, Manuel J; Fay, Michael F

    2016-04-01

    Orchids in the genus Ophrys represent extraordinary cases of tight coevolution between plants and their pollinators, and as a result, they present opportunities for studying hybridization, or a lack thereof, during speciation. However, few studies assess the real effect of hybridization in diversification. The three most representative species of section Pseudophrys in the western Iberian Peninsula-O. dyris, O. fusca, and O. lutea-were chosen to study evolutionary relationships and examine speciation. Using eight specific nuclear microsatellite loci, 357 individuals from 28 locations were studied; 142 of these samples were also studied with four plastid microsatellite loci. Data were analyzed using Bayesian cluster analysis, a median-joint network, and multivariate analysis. Many O. dyris and O. fusca specimens had three or four alleles and were therefore treated as tetraploid. Ophrys dyris is poorly genetically separated from O. fusca, and pure populations are rare. Ophrys fusca and O. lutea are distinct, but hybrids/introgressed individuals were detected in most of the populations and supported by plastid haplotypes. Ophrys fusca is subdivided into three well-delimited genetic lineages with a strict geographic correspondence confirmed by plastid haplotypes. Because postzygotic barriers are weak, leakage in this highly specialized orchid-pollinator system contributes to hybridization and introgression. These leakages may have occurred during periods of past climate change, promoting homogenization and the potential for generations of new biodiversity via production of novel genotypes/phenotypes interacting with pollinators. © 2016 Botanical Society of America.

  6. PET/MRI of Hepatic 90Y Microsphere Deposition Determines Individual Tumor Response

    Energy Technology Data Exchange (ETDEWEB)

    Fowler, Kathryn J. [Washington University, Department of Radiology (United States); Maughan, Nichole M. [Washington University, Department of Biomedical Engineering (United States); Laforest, Richard [Washington University, Department of Nuclear Medicine (United States); Saad, Nael E. [Washington University, Department of Radiology (United States); Sharma, Akash [Washington University, Department of Nuclear Medicine (United States); Olsen, Jeffrey; Speirs, Christina K.; Parikh, Parag J., E-mail: parikh@wustl.edu [Washington University, Department of Radiation Oncology (United States)

    2016-06-15

    PurposeThe purpose of our study is to determine if there is a relationship between dose deposition measured by PET/MRI and individual lesion response to yttrium-90 ({sup 90}Y) microsphere radioembolization.Materials and Methods26 patients undergoing lobar treatment with {sup 90}Y microspheres underwent PET/MRI within 66 h of treatment and had follow-up imaging available. Adequate visualization of tumor was available in 24 patients, and contours were drawn on simultaneously acquired PET/MRI data. Dose volume histograms (DVHs) were extracted from dose maps, which were generated using a voxelized dose kernel. Similar contours to capture dimensional and volumetric change of tumors were drawn on follow-up imaging. Response was analyzed using both RECIST and volumetric RECIST (vRECIST) criteria.ResultsA total of 8 hepatocellular carcinoma (HCC), 4 neuroendocrine tumor (NET), 9 colorectal metastases (CRC) patients, and 3 patients with other metastatic disease met inclusion criteria. Average dose was useful in predicting response between responders and non-responders for all lesion types and for CRC lesions alone using both response criteria (p < 0.05). D70 (minimum dose to 70 % of volume) was also useful in predicting response when using vRECIST. No significant trend was seen in the other tumor types. For CRC lesions, an average dose of 29.8 Gy offered 76.9 % sensitivity and 75.9 % specificity for response.ConclusionsPET/MRI of {sup 90}Y microsphere distribution showed significantly higher DVH values for responders than non-responders in patients with CRC. DVH analysis of {sup 90}Y microsphere distribution following treatment may be an important predictor of response and could be used to guide future adaptive therapy trials.

  7. The study of 90 deg. gamma ray production cross sections for interactions of 14.9 Mev neutrons with C, Al, V, Fe, Co, Nb samples

    International Nuclear Information System (INIS)

    Tang Lin; Yan Yiming; Zhou Hongyu; Wen Shenlin; Wang Qi; Sun Shuxu; Ding Xiaoji; Wang Wanhong

    1987-04-01

    The (n,xr) reactions have been studied for incident neutron energy 14.9 Mev and for the samples C, Al, V, Fe, Co and Nb. The pulsed beam Time-of-Flight technique was adopted to discriminate neutrons and de-excitation r-rays and to improve the background conditions of the experiment. 90 deg. differential gamma production cross sections as the results of experiments are presented. Due to very low background many new r-rays peaks have been obtained. (author). 14 refs, 12 figs, 1 tab

  8. Stereochemistry of Endogenous Palmitic Acid Ester of 9-Hydroxystearic Acid and Relevance of Absolute Configuration to Regulation.

    Science.gov (United States)

    Nelson, Andrew T; Kolar, Matthew J; Chu, Qian; Syed, Ismail; Kahn, Barbara B; Saghatelian, Alan; Siegel, Dionicio

    2017-04-05

    Lipids have fundamental roles in the structure, energetics, and signaling of cells and organisms. The recent discovery of fatty acid esters of hydroxy fatty acids (FAHFAs), lipids with potent antidiabetic and anti-inflammatory activities, indicates that our understanding of the composition of lipidome and the function of lipids is incomplete. The ability to synthesize and test FAHFAs was critical in elucidating the roles of these lipids, but these studies were performed with racemic mixtures, and the role of stereochemistry remains unexplored. Here, we synthesized the R- and S- palmitic acid ester of 9-hydroxystearic acid (R-9-PAHSA, S-9-PAHSA). Access to highly enantioenriched PAHSAs enabled the development of a liquid chromatography-mass spectrometry (LC-MS) method to separate and quantify R- and S-9-PAHSA, and this approach identified R-9-PAHSA as the predominant stereoisomer that accumulates in adipose tissues from transgenic mice where FAHFAs were first discovered. Furthermore, biochemical analysis of 9-PAHSA biosynthesis and degradation indicate that the enzymes and pathways for PAHSA production are stereospecific, with cell lines favoring the production of R-9-PAHSA and carboxyl ester lipase (CEL), a PAHSA degradative enzyme, selectively hydrolyzing S-9-PAHSA. These studies highlight the role of stereochemistry in the production and degradation of PAHSAs and define the endogenous stereochemistry of 9-PAHSA in adipose tissue. This information will be useful in the identification and characterization of the pathway responsible for PAHSA biosynthesis, and access to enantiopure PAHSAs will elucidate the role of stereochemistry in PAHSA activity and metabolism in vivo.

  9. ROV90 - A prototype autonomous inspection vehicle

    Energy Technology Data Exchange (ETDEWEB)

    Roedseth, Oe.J.; Hallset, J.O.

    1991-04-01

    Simple autonomous inspection vehicles are suitable for operations where the cost, danger to humans, or area of operation prohibits the use of conventional underwater technology. Autonomous vehicles are, however, in their infancy and few such vehicles are available. There are still some problems to be overcome before this technology becomes useful in commercial applications. We have built ROV90 to investigate these problems. It is a test bed for experimenting with the different parts of an autonomous underwater vehicle. ROV90 will be able to autonomously follow prominent features in the real world, man made or natural. Examples are pipelines or walls in tunnels. ROV90 is tethered, but we are planning to use experience and results from ROV90 to develop av ''real'' autonomous underwater vehicle (AUV) called PISCIS. 11 refs., 8 figs.

  10. 90Y microsphere therapy: does 90Y PET/CT imaging obviate the need for 90Y Bremsstrahlung SPECT/CT imaging?

    Science.gov (United States)

    Zade, Anand A; Rangarajan, Venkatesh; Purandare, Nilendu C; Shah, Sneha A; Agrawal, Archi R; Kulkarni, Suyash S; Shetty, Nitin

    2013-11-01

    Transarterial radioembolization using Y microspheres is a novel therapeutic option for inoperable hepatic malignancies. As these spheres are radiolucent, real-time assessment of their distribution during the infusion process under fluoroscopic guidance is not possible. Bremsstrahlung radiations arising from 90Y have conventionally been used for imaging its biodistribution. Recent studies have proved that sources of 90Y also emit positrons, which can further be used for PET/computed tomography (CT) imaging. This study aimed to assess the feasibility of 90Y PET/CT imaging in evaluating microsphere distributions and to compare its findings with those of Bremsstrahlung imaging. Thirty-five sessions of 90Y microsphere transarterial radioembolization were performed on 30 patients with hepatic malignancies. 90Y PET/CT imaging was performed within 3 h of therapy. Bremsstrahlung imaging was also performed for each patient. The imaging findings were compared for concordance in the distribution of microspheres. Exact one-to-one correspondence between 90Y PET/CT imaging and 90Y Bremsstrahlung imaging was observed in 97.14% of cases (i.e. in 34/35 cases). Discordance was observed only in one case in which 90Y PET/CT imaging resolved the microsphere uptake in the inferior vena cava tumor thrombus, which was, however, not visualized on Bremsstrahlung imaging. There is good concordance in the imaging findings of 90Y PET/CT and 90Y Bremsstrahlung imaging. 90Y PET/CT imaging scores over the conventionally used Bremsstrahlung imaging in terms of better resolution, ease of technique, and comparable image acquisition time. This makes it a preferred imaging modality for assessment of the distribution of 90Y microspheres.

  11. 47 CFR 90.115 - Foreign government and alien eligibility.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Foreign government and alien eligibility. 90... government and alien eligibility. (a) No station authorization in the radio services governed by this part....9(c) of this chapter) if such entity is: (1) An alien or the representative of any alien; (2) A...

  12. 90Sr- 90Y and 89Sr beta radioactivity measurement in milk samples using a proportional counter

    International Nuclear Information System (INIS)

    Mananes, A.; Perez Santos, C.; Martinez Churiaque, F.

    1987-01-01

    A thin window glas flow proportional counter is used to measure the 90 Sr- 90 Y and 89 Sr beta radioactivity in milk samples. A chemical procedure is used to separate strontium-yttrium from the other radionuclides present in milk. A calculation of the total efficiency of the system is performed which includes an empirical estimation of the backscattering factor. The calibration of the whole process allows the determination of the 90 Sr activity within 10% relative error in spite of uncertainties in the recovery yields of strontium and yttrium. No 89 Sr activity has been detected, and the mean value obtained for the 90 Sr activity in nine milk samples of Cantabria is 0.115 Bq/1 with a minimum detectable activity of 0.0105 Bq. (author) 18 refs

  13. 90Sr-90Y radionuclide generator based on ionex chromatography. Part 1 - project

    International Nuclear Information System (INIS)

    Miler, V.; Budsky, F.; Malek, Z.

    2003-09-01

    This part contains a proposal for the generator column design, materials to be used (chemicals, ionexes) and technological procedures. The proposal was inspired by the 90 Sr- 90 Y generator operated by Zfk Rossendorf. The aim was to develop and launch a generator for the preparation of carrier-free 90 Y in the form of [ 90 Y] chloride solution in dilute hydrochloric acid. The separation of Y from Sr is based on ionex chromatography by sorbing the two radionuclides on a catex. While Sr remains sorbed, 90 Y is eluted with lithium citrate. During this process, 90 Y is bonded in a citrate complex which, having a negative charge, is subsequently trapped by an anex. A guard column is inserted before the anex column to trap any traces of 90 Sr. 90 Y is eluted from the anex in the yttrium chloride form by using dilute hydrochloric acid. The product from the generator can be used for the preparation of [ 90 Y] - Fe colloid injection or [ 90 Y] - yttrium citrate injection for intra-articular application or for the development of monoclonal antibodies and peptides

  14. 21 CFR 520.90a - Ampicillin capsules.

    Science.gov (United States)

    2010-04-01

    .... Use of the drug is contraindicated in animals with a history of an allergic reaction to any of the... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Ampicillin capsules. 520.90a Section 520.90a Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL...

  15. Reversibility of substrate adsorption for the cellulases Cel7A, Cel6A and Cel7B from H. jecorina

    DEFF Research Database (Denmark)

    Pellegrini, Vanessa de Oliveira Arnoldi; Lei, Nina; Kysaram, Madhuri

    2014-01-01

    Adsorption of cellulases on the cellulose surface is an integral part of the catalytic mechanism, and a detailed description of the adsorption process is therefore required for a fundamental understanding of this industrially important class of enzymes. However, the mode of adsorption has proven...

  16. Large-scale purification of 90Sr from nuclear waste materials for production of 90Y, a therapeutic medical radioisotope.

    Science.gov (United States)

    Wester, Dennis W; Steele, Richard T; Rinehart, Donald E; DesChane, Jaquetta R; Carson, Katharine J; Rapko, Brian M; Tenforde, Thomas S

    2003-07-01

    A major limitation on the supply of the short-lived medical isotope 90Y (t1/2 = 64 h) is the available quantity of highly purified 90Sr generator material. A radiochemical production campaign was therefore undertaken to purify 1,500 Ci of 90Sr that had been isolated from fission waste materials. A series of alkaline precipitation steps removed all detectable traces of 137Cs, alpha emitters, and uranium and transuranic elements. Technical obstacles such as the buildup of gas pressure generated upon mixing large quantities of acid with solid 90Sr carbonate were overcome through safety features incorporated into the custom-built equipment used for 90Sr purification. Methods are described for analyzing the chemical and radiochemical purity of the final product and for accurately determining by gravimetry the quantities of 90Sr immobilized on stainless steel filters for future use.

  17. Large-scale purification of 90Sr from nuclear waste materials for production of 90Y, a therapeutic medical radioisotope

    International Nuclear Information System (INIS)

    Wester, D.W.; Steele, R.T.; Rinehart, D.E.; DesChane, J.R.; Carson, K.J.; Rapko, B.M.; Tenforde, T.S.

    2003-01-01

    A major limitation on the supply of the short-lived medical isotope 90 Y (t 1/2 =64 h) is the available quantity of highly purified 90 Sr generator material. A radiochemical production campaign was therefore undertaken to purify 1500 Ci of 90 Sr that had been isolated from fission waste materials. A series of alkaline precipitation steps removed all detectable traces of 137 Cs, alpha emitters, and uranium and transuranic elements. Technical obstacles such as the buildup of gas pressure generated upon mixing large quantities of acid with solid 90 Sr carbonate were overcome through safety features incorporated into the custom-built equipment used for 90 Sr purification. Methods are described for analyzing the chemical and radiochemical purity of the final product and for accurately determining by gravimetry the quantities of 90 Sr immobilized on stainless steel filters for future use

  18. 30 CFR 90.3 - Part 90 option; notice of eligibility; exercise of option.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Part 90 option; notice of eligibility; exercise of option. 90.3 Section 90.3 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF... DEVELOPMENT OF PNEUMOCONIOSIS General § 90.3 Part 90 option; notice of eligibility; exercise of option. (a...

  19. Development of 90Sr/90Y Generator Systems Based on SLM Techniques for Radiolabelling of Therapeutic Biomolecules with 90Y. Chapter 14

    International Nuclear Information System (INIS)

    Thu, N.T.; Van Dong, D.; Van Cuong, B.; Van Khoa, C.; Cam Hoa, V.T.

    2015-01-01

    Yttrium-90 is one of the most useful radionuclides for radioimmunotherapeutic applications, especially for labelling peptides and antibodies. Studies were carried out to develop a 90 Sr/ 90 Y generator system based on the SLM technique. Two stages of 90 Sr/ 90 Y generator systems were developed at different activity levels of 5, 20, 50 and 100 mCi and operated with semiautomation in sequential mode. In the first stage of the system, PC88A based SLM was used, which transported 90 Y from a nitric acid medium containing 0.01–4M HNO 3 . In the second stage, the 90 Y from the first stage was transferred to the first compartment of the second stage using carbamoylmethyl phosphine oxide (CMPO) based SLM where 1M acetic acid was used as the receiving phase for 90 Y. Quality control was carried out for the products of 90 Y using EPC with paper chromatography and Tec control chromatography. Peptides and antibodies were labelled using the 90 Y product obtained from the generator developed in house. (author)

  20. Shortcomings of InSAR for studying megathrust earthquakes: The case of the M w 9.0 Tohoku-Oki earthquake

    KAUST Repository

    Feng, Guangcai

    2012-05-28

    Interferometric Synthetic Aperture Radar (InSAR) observations are sometimes the only geodetic data of large subduction-zone earthquakes. However, these data usually suffer from spatially long-wavelength orbital and atmospheric errors that can be difficult to distinguish from the coseismic deformation and may therefore result in biased fault-slip inversions. To study how well InSAR constrains fault-slip of large subduction zone earthquakes, we use data of the 11 March 2011 Tohoku-Oki earthquake (Mw9.0) and test InSAR-derived fault-slip models against models constrained by GPS data from the extensive nationwide network in Japan. The coseismic deformation field was mapped using InSAR data acquired from multiple ascending and descending passes of the ALOS and Envisat satellites. We then estimated several fault-slip distribution models that were constrained using the InSAR data alone, onland and seafloor GPS/acoustic data, or combinations of the different data sets. Based on comparisons of the slip models, we find that there is no real gain by including InSAR observations for determining the fault slip distribution of this earthquake. That said, however, some of the main fault-slip patterns can be retrieved using the InSAR data alone when estimating long wavelength orbital/atmospheric ramps as a part of the modeling. Our final preferred fault-slip solution of the Tohoku-Oki earthquake is based only on the GPS data and has maximum reverse- and strike-slip of 36.0 m and 6.0 m, respectively, located northeast of the epicenter at a depth of 6 km, and has a total geodetic moment is 3.6 × 1022 Nm (Mw 9.01), similar to seismological estimates.

  1. Synthesis and functioning of the colicin E1 lysis protein: Comparison with the colicin A lysis protein

    International Nuclear Information System (INIS)

    Cavard, D.

    1991-01-01

    The colicin E1 lysis protein, CelA, was identified as a 3-kDa protein in induced cells of Escherichia coli K-12 carrying pColE1 by pulse-chase labeling with either [ 35 S]cysteine or [ 3 H]lysine. This 3-kDa protein was acylated, as shown by [2- 3 H]glycerol labeling, and seemed to correspond to the mature CelA protein. The rate of modification and processing of CelA was different from that observed for Cal, the colicin A lysis protein. In contrast to Cal, no intermediate form was detected for CelA, no signal peptide accumulated, and no modified precursor form was observed after globomycin treatment. Thus, the rate of synthesis would not be specific to lysis proteins. Solubilization in sodium dodecyl sulfate of the mature forms of both CelA and Cal varied similarly at the time of colicin release, indicating a change in lysis protein structure. This particular property would play a role in the mechanism of colicin export. The accumulation of the signal peptide seems to be a factor determining the toxicity of the lysis proteins since CelA provoked less cell damage than Cal. Quasi-lysis and killing due to CelA were higher in degP mutants than in wild-type cells. They were minimal in pldA mutants

  2. The transfer of strontium-90 and caesium-137 to milk in a dairy herd grazing near a major nuclear installation

    Energy Technology Data Exchange (ETDEWEB)

    Sumerling, T J; Dodd, N J; Green, N

    1984-03-01

    A field investigation of the transfer of artificially produced radionuclides in the pasture--cow--milk pathway has been made at a farm close to the nuclear fuel reprocessing installation at Sellafield on the north-west coast of England. This paper reports results from analyses of samples collected during 1981, reports transfers coefficients for /sup 90/Sr and /sup 137/Cs from various types of feed to milk, and discusses factors that affect the transfer of these radionuclides. It is shown that during 1981 a large proportion of the /sup 90/Sr and /sup 137/Cs consumed by cattle grazing near Sellafield was derived from activity deposited in previous years. Transfer coefficients to milk, Fm, have been derived which are within the ranges of those observed in tracer and fallout studies. There are significant seasonal changes in transfer. For /sup 90/Sr, values of Fm between 9 X 10(-4)d 1(-1) and 4 X 10(-3)d 1(-1) have been obtained. It is concluded that this large range arises because daily intakes of /sup 90/Sr by the herd during the winter months are lower (by a factor of about 3) than intakes during the summer months and that the concentration of /sup 90/Sr in milk is not in equilibrium with intake, that is, the concentration of /sup 90/Sr in milk is maintained both by recent intakes and by remobilisation of activity that has been accumulated in bone from earlier intakes. For /sup 137/Cs, values of Fm between 3 X 10(-3)d 1(-1) and 9 X 10(-3)d 1(-1) have been obtained. It is concluded that this range most probably occurs because during the summer months, when the cows are grazing, a substantial proportion of the /sup 137/Cs intake is associated with soil on the surface of herbage and that, in this form, the /sup 137/Cs is less available for uptake from the digestive tract of the cows.

  3. Characterization of a Newly Developed Contrast Enhancement Material for G-line Exposure

    Science.gov (United States)

    Nakase, Makoto; Niki, Hirokazu; Satoh, Takashi; Kumagae, Akitoshi

    1987-02-01

    The bleaching characteristics for a contrast enhancement layer (CEL) material were succesfully described by parameters A, B and C; these were used for the modeling of a positive photoresist exposure. As a result, it was clarified that both the A and C values should be large, but the B value must be as small as possible. According to the obtained information, a new CEL material was proposed, which consists of the diazonium compound and the alkyl modified phenol resin. Using the composed CEL material, a submicron resist pattern with a steep profile was obtained. Furthermore, it was found that the development latitude increases, but that the exposure latitude does not change upon using the CEL.

  4. Superconducting Gravimeters Detect Gravity Fluctuations Induced by Mw 5.7 Earthquake Along South Pacific Rise Few Hours Before the 2011 Mw 9.0 Tohoku-Oki Earthquake

    Directory of Open Access Journals (Sweden)

    Keliang Zhang Jin Ma

    2014-01-01

    Full Text Available Gravity changes sometimes appear before a big earthquake. To determine the possible sources is important for recognizing the mechanism and further geodynamic studies. During the first two hours on March 11 before the Mw 9.0 Tohoku-Oki earthquake, the non-tidal gravity time series of superconducting gravimeters worldwide showed low-frequency (< 0.10 Hz fluctuations with amplitude of ~1 to 4 × 10-8 ms-2 lasting ~10 - 20 minutes. Through comparing global seismicity with the arrival times of seismic waves, we find that the fluctuations were induced by the Mw 5.7 earthquake that occurred at 0:14:54.68 at (53.27°S, 118.18°W along the eastern South Pacific Rise. Several body waves such as P, S are clearly recorded in the station with ~400 km distance to the hypocenter. The fluctuations are in response to the waves that propagate with a velocity of about 4 km s-1. Their amplitudes are proportional to the inverse of the epicentral distances even though the fluctuations of European sites were overlapped with waves associated with a smaller, i.e., Mw 2.6, event in Europe during this period. That is, the Mw 5.7 earthquake induced remarkable gravity fluctuations over long distances at stations all over the world. As such, the foreshocks with larger magnitudes occurred before the Mw 9.0 earthquake would have more significant influence on the gravity recordings and the seismic-wave induced component should be removed during the analysis of anomalies prior to a great earthquake in future studies.

  5. Strontium-90 and cesium-137 in sea sediments (from May 1984 to Sep 1984)

    International Nuclear Information System (INIS)

    1984-01-01

    Strontium-90 and cesium-137 monitoring results are presented for sea sediment samples of 12 sampling points located all over Japan from Tomari, Hokkaido to Kinnakagusuku Bay, Okinawa. The samples were collected by considering of enough sea water depth, no significant sedimental movement and sediment characteristics, and by employing a conventional sampling device. Approximately 4 kg-wet sample was dried and was passed through a 20 cm mesh sieve. After adding of strontium and cesium carriers, strontium-90 and cesium-137 were leached with a hot hydrochloric acid solution. The leachate was treated by ion exchange and coprecipitation to concentrate and isolate strontium-90 or cesium-137. Radiation counting was carried out by employing a low background beta counter usually for 60 minutes for the samples of strontium carbonate or cesium chloroplatinate. Determined strontium-90 contents in sea sediment were distributed from 0 +- 2.7 pCi/kg-dry (Mutsu Bay, Aomori, Yamaguchi Bay, Yamaguchi) to 14 +- 3.2 pCi/kg-dry (Mutsu Bay), and those of cesium-137 were from 9 +- 3.5 pCi/kg-dry (Mutsu Bay) to 250 +- 9 pCi/kg-dry (Off-Niigata Port, Niigata). Local variation of the contents of these radionuclides was very large, and for seasonal variation, it was also found large for the both nuclides content in the Mutsu Bay samples of May, 1984 and August 1984, as for strontium-90, 0 +- 2.7 pCi/kg and 14 +- 3.2 pCi/kg, for cesium-137, 9 +- 3.5 pCi/kg and 200 +- 8 pCi/kg, respectively. (Takagi, S.)

  6. Cellulose synthases: new insights from crystallography and modeling.

    Science.gov (United States)

    Slabaugh, Erin; Davis, Jonathan K; Haigler, Candace H; Yingling, Yaroslava G; Zimmer, Jochen

    2014-02-01

    Detailed information about the structure and biochemical mechanisms of cellulose synthase (CelS) proteins remained elusive until a complex containing the catalytic subunit (BcsA) of CelS from Rhodobacter sphaeroides was crystalized. Additionally, a 3D structure of most of the cytosolic domain of a plant CelS (GhCESA1 from cotton, Gossypium hirsutum) was produced by computational modeling. This predicted structure contributes to our understanding of how plant CelS proteins may be similar and different as compared with BcsA. In this review, we highlight how these structures impact our understanding of the synthesis of cellulose and other extracellular polysaccharides. We show how the structures can be used to generate hypotheses for experiments testing mechanisms of glucan synthesis and translocation in plant CelS. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Simultaneous Quantitation of Advanced Glycation End Products in Soy Sauce and Beer by Liquid Chromatography-Tandem Mass Spectrometry without Ion-Pair Reagents and Derivatization.

    Science.gov (United States)

    Nomi, Yuri; Annaka, Hironori; Sato, Shinji; Ueta, Etsuko; Ohkura, Tsuyoshi; Yamamoto, Kazuhiro; Homma, Seiichi; Suzuki, Emiko; Otsuka, Yuzuru

    2016-11-09

    The aim of this study was to develop a simple and sensitive method to analyze several advanced glycation end products (AGEs) simultaneously using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and to apply this method to the quantitation of AGEs in brown-colored foods. The developed method enabled to separate and quantitate simultaneously seven AGEs, and was applied to the determination of free AGEs contained in various kinds of soy sauce and beer. The major AGEs in soy sauce and beer were N ε -carboxymethyllysine (CML), N ε -carboxyethyllysine (CEL), and N δ -(5-hydro-5-methyl-4-imidazolon-2-yl)ornithine (MG-H1). Using the developed LC-MS/MS method, recovery test on soy sauce and beer samples showed the recovery values of 85.3-103.9% for CML, 95.9-107.4% for CEL, and 69.5-123.2% for MG-H1. In particular, it is the first report that free CML, CEL, and MG-H1 were present in beer. Furthermore, long-term storage and heating process of soy sauce increased CML and MG-H1.

  8. A Finnish validation study of the SCL-90.

    Science.gov (United States)

    Holi, M M; Sammallahti, P R; Aalberg, V A

    1998-01-01

    The Symptom Check-List-90 (SCL-90) is a widely used psychiatric questionnaire which has not yet been validated in Finland. We investigated the utility of the translated version of the SCL-90 in the Finnish population, and set community norms for it. The internal consistency of the original subscales was checked and found to be good. Discriminant function analysis, based on the nine original subscales, showed that the power of the SCL-90 to discriminate between patients and the community is good. Factor analysis of the items of the questionnaire yielded a very strong unrotated first factor, suggesting that a general factor may be present. This together with the fact that high intercorrelations were found between the nine original subscales suggests that the instrument is not multidimensional. The SCL-90 may be useful in a research setting as an instrument for measuring the change in symptomatic distress, or as a screening instrument. The American community norms should be used with caution, as the Finnish community sample scored consistently higher on all subscales.

  9. Country report: Vietnam. Setting Up of a {sup 90}Sr/{sup 90}Y Generator System Based on Supported Liquid Membrane (SLM) Technique and Radiolabeling of Eluted {sup 90}Y with Biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Thu, Nguyen Thi; Dong, Duong Van; Cuong, Bui Van; Khoa, Chu Van [Vietnam Atomic Energy Commission (VAEC), Nuclear Research Institute, Dalat (Viet Nam)

    2010-07-01

    In the course of participating in the IAEA-CRP during the last two years, Vietnam has achieved the goal of setting up a {sup 90}Sr/{sup 90}Y generator system based on Supported Liquid Membrane (SLM) technique and also radiolabeling of the eluted {sup 90}Y with antibody, peptides and albumin. A two stage SLM based {sup 90}Sr-{sup 90}Y generator was set up in-house to generate carrier-free {sup 90}Y at different activity levels viz. 5, 20, 50 mCi. The generator system was operated in sequential mode in which 2-ethylhexyl 2-ethylhexyl phosphonic acid (PC88A) based SLM was used in the first stage for the transport {sup 90}Y in 4.0 M nitric acid from source phase where {sup 90}Sr-{sup 90}Y equilibrium mixture is placed in nitric acid medium at pH to 1-2. In the second stage, octyl (phenyl)-N,N-diisobutylcarbamoylmethyl phosphine oxide (CMPO) based SLM was used for the transport of {sup 90}Y selectively to 1.0 M acetic acid which is the best medium for radiolebeling. The eluted {sup 90}Y from the generator was tested for the presence of any traces of {sup 90}Sr using the Extraction Paper Chromatography (EPC) and was found suitable for radiolabeling. The generator system could be upgraded to 100 mCi level successfully due to an expert mission from India through IAEA. The {sup 90}Y product obtained from the generator system was used for radiolabeling of antibody and peptides viz. Rituximab, DOTATATE and albumin particles under different experimental conditions. A new chromatography system could be developed for analyzing {sup 90}Y labeled albumin using the TAE buffer as mobile phase in PC and ITLC.

  10. A 90 GHz photoinjector

    International Nuclear Information System (INIS)

    Palmer, D.T.; Hogan, M.J.; Ferrario, M.; Serafini, L.

    1999-01-01

    Photocathode rf guns depend on mode locked laser systems to produce an electron beam at a given phase of the rf. In general, the laser pulse is less than σ 2 = 10'' of rf phase in length and the required stability is on the order of Δφ = 1 At 90 GHz (W-band), these requirements correspond to σ 2 = 333 fsec and Δφ = 33 fsec. Laser system with pulse lengths in the fsec regime are commercially available, the timing stability is a major concern. It is proposed a multi-cell W-band photoinjector that does not require a mode locked laser system. Thereby eliminating the stability requirements at W-band. The laser pulse is allowed to be many rf periods long. In principle, the photoinjector can now be considered as a thermionic rf gun. Instead of using an alpha magnet to compress the electron bunch, which would have a detrimental effect on the transverse hase space quality due to longitudinal phase space mixing, it is here proposed to use long pulse laser system and a pair of undulators to produce a low emittance, high current, ultra-short electron bunch for beam dynamics experiments in the 90 GHz regime

  11. Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from Trichoderma atroviride, T. reesei and T. harzianum.

    Science.gov (United States)

    Borisova, Anna S; Eneyskaya, Elena V; Jana, Suvamay; Badino, Silke F; Kari, Jeppe; Amore, Antonella; Karlsson, Magnus; Hansson, Henrik; Sandgren, Mats; Himmel, Michael E; Westh, Peter; Payne, Christina M; Kulminskaya, Anna A; Ståhlberg, Jerry

    2018-01-01

    The ascomycete fungus Trichoderma reesei is the predominant source of enzymes for industrial conversion of lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH) Tre Cel7A constitutes nearly half of the enzyme cocktail by weight and is the major workhorse in the cellulose hydrolysis process. The orthologs from Trichoderma atroviride ( Tat Cel7A) and Trichoderma harzianum ( Tha Cel7A) show high sequence identity with Tre Cel7A, ~ 80%, and represent naturally evolved combinations of cellulose-binding tunnel-enclosing loop motifs, which have been suggested to influence intrinsic cellobiohydrolase properties, such as endo-initiation, processivity, and off-rate. The Tat Cel7A, Tha Cel7A, and Tre Cel7A enzymes were characterized for comparison of function. The catalytic domain of Tat Cel7A was crystallized, and two structures were determined: without ligand and with thio-cellotriose in the active site. Initial hydrolysis of bacterial cellulose was faster with Tat Cel7A than either Tha Cel7A or Tre Cel7A. In synergistic saccharification of pretreated corn stover, both Tat Cel7A and Tha Cel7A were more efficient than Tre Cel7A, although Tat Cel7A was more sensitive to thermal inactivation. Structural analyses and molecular dynamics (MD) simulations were performed to elucidate important structure/function correlations. Moreover, reverse conservation analysis (RCA) of sequence diversity revealed divergent regions of interest located outside the cellulose-binding tunnel of Trichoderma spp. GH7 CBHs. We hypothesize that the combination of loop motifs is the main determinant for the observed differences in Cel7A activity on cellulosic substrates. Fine-tuning of the loop flexibility appears to be an important evolutionary target in Trichoderma spp., a conclusion supported by the RCA data. Our results indicate that, for industrial use, it would be beneficial to combine loop motifs from Tat Cel7A with the thermostability features of Tre Cel7A. Furthermore

  12. Development of a dosimetric system for {sup 90}Sr + {sup 90}Y betatherapy applicators; Desenvolvimento de um sistema de dosimetria para aplicadores de betaterapia de {sup 90}Sr + {sup 90}Y

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Talita Salles

    2010-07-01

    The {sup 90}Sr+{sup 90}Y applicators, used in betatherapy for prevention of keloids and pterigium, are imported and many times their dosimetric features are shown only in an illustrated form by the manufacturers. The exhaustive routine of the medical physicists in the clinic do not make possible the accomplishment of procedures for the confirmation of these parameters. This work presents the development of a methodology for the dosimetry of {sup 90}Sr+{sup 90}Y betatherapy applicators. The Monte Carlo code MCNP5 was used for the simulation of the percentage depth dose curves and dose distribution profiles produced by these applicators. The experimental measurements of the radial and axial radiation attenuation, have been done with a mini-extrapolation chamber, thermoluminescent dosimeters and radiographic films. The experimental results have been compared with the simulated values. Both percentage depth dose curves and the radial dose profiles, the theoretical and the experimental ones, have presented good agreement, which may validate the use of the MCNP5 for these simulations, confirming the viability of the usage of this method in procedures of beta emitter sources dosimetry. (author)

  13. Environmental 90Sr measurements

    Science.gov (United States)

    Paul, M.; Berkovits, D.; Cecil, L.D.; Feldstein, H.; Hershkowitz, A.; Kashiv, Y.; Vogt, S.

    1997-01-01

    90Sr (T1/2 = 28.5 years) is a long-lived radionuclide produced in nuclear fission. Fast radiochemical detection of 90Sr in environmental samples is not feasible using current analytical methods. Accelerator Mass Spectrometry (AMS) measurements of 90Sr were made with the Rehovot 14UD Pelletron accelerator at a terminal voltage of 11 or 12 MV using our standard detection system. Injection of hydride ions (SrH3-) was chosen owing to high beam intensity and low Coulomb explosion effects. 90Sr ions were identified and discriminated from isobaric 90Zr by measuring time of flight, total energy and three independent energy-loss signals in an ionization chamber. A reference sample and a ground-water sample were successfully measured. The detection limit determined for a laboratory blank by the residual counts in the 90Sr region is 90Sr/Sr = 3 ?? 10-13, corresponding in practice to (2-4) ?? 10790Sr atoms or about 0.5-1 pCi/L in environmental water samples.

  14. Verification dosimetry of intravascular 90Sr/90Y source trains

    International Nuclear Information System (INIS)

    Sharma, S.D.; Shanta, A.; Tripathi, U.B.; Bhatt, B.C.

    2001-01-01

    90 Sr/ 90 Y source trains (Novoste Beta-Cath System) are currently under clinical trials in India and abroad for intracoronary brachytherapy for prevention of restenosis. Each source train of the Beta-Cath system is supplied with a source certificate giving dose rate at the reference distance of 2 mm from the centerline of the source train. It is essential that the user should check dose rates of brachytherapy sources before its application on the patients. Dose rates and depth dose measurements for 90 Sr/ 90 Y source trains of active length 40 mm using radiochromic films in a tissue equivalent phantom have been carried out. The objectives of these measurements were (1) to verify the dose rates stated in the source certificate, and (2) to obtain relative depth dose data for treatment planning. This paper presents the results of these measurements

  15. Assessment of celecoxib poly(lactic-co-glycolic) acid nanoformulation on drug pharmacodynamics and pharmacokinetics in rats.

    Science.gov (United States)

    Harirforoosh, S; West, K O; Murrell, D E; Denham, J W; Panus, P C; Hanley, G A

    2016-11-01

    Celecoxib (CEL) is a nonsteroidal anti-inflammatory drug (NSAID) showing selective cycloxygenase-2 inhibition. While effective as a pain reducer, CEL exerts some negative influence on renal and gastrointestinal parameters. This study examined CEL pharmacodynamics and pharmacokinetics following drug reformulation as a poly(lactic-co-glycolic) acid nanoparticle (NP). Rats were administered either vehicle (VEH) (methylcellulose solution), blank NP, 40 mg/kg CEL in methylcellulose, or an equivalent NP dose (CEL-NP). Plasma and urine (over 12 hrs) samples were collected prior to and post-treatment. The mean percent change from baseline of urine flow rate along with electrolyte concentrations in plasma and urine were assessed based on 100 g body weight. Using tissues collected 24 hrs post-treatment, gastrointestinal inflammation was estimated through duodenal and gastric prostaglandin E2 (PGE2) and duodenal myeloperoxidase (MPO) levels; while kidney tissue was examined for dilatation and necrosis. CEL concentration was assayed in renal tissue and plasma utilizing high-performance liquid chromatography. Although there were significant changes when comparing CEL and CEL-NP to VEH in plasma sodium concentration and potassium excretion rate, there was no significant variation between CEL and CEL-NP. There was a significant reduction of protective duodenal PGE2 in CEL compared to VEH (p = 0.0088) and CEL-NP (p = 0.02). In the CEL-NP formulation, t1/2, Cmax, AUC0-∞, and Vd/F increased significantly when compared to CEL. At the observed dosage and duration, CEL-NP may not affect CEL-associated electrolyte parameters in either plasma or urine; however, it does provide increased systemic exposure while potentially alleviating some gastrointestinal outcomes related to inflammation.

  16. Evaluation of mobility potential of {sup 90}Sr in Brazilian soils; Avaliacao do potencial de mobilidade do {sup 90}Sr em solos brasileiros

    Energy Technology Data Exchange (ETDEWEB)

    Viana, Aline Gonzalez

    2007-07-01

    In this document, the transfer mechanisms of {sup 90}Sr were studied in soil-plant systems from Brazil, integrating field and laboratory experiments. Some soil classes, with different physical and chemical properties, relevant to Brazil were selected. The soil classes included in this study were Oxisol, Alfisol and Nitisol. These soils were artificially contaminated with {sup 90}Sr for crop cultivation in lysimeters located at the experimental area of the Instituto de Radioprotecao e Dosimetria. The selected crops chosen to the root uptake studies were: corn (Zea mays, L.), cabbage (Brassica oleracea) and radish (Raphanus sativus L.). These cultures were chosen because they have varying absorption rates due to differences in the physiologic structures analyzed: grain, leaf and root. This study identified Nitisol, which contains clay of high activity, as having smaller transfer factor for all crops and these values were in the same order of magnitude as temperate climate values reported in the specialized literature: FT{sub corn}: 5,78E-3 (n=2); FT{sub radish}: 1,78 E0 (n=2); FT {sub cabbage}: 9,16E-1 (n=2). All other soils presented higher TF values, ranging from 1,93E0 to 4,06 E + 01 for radish (n=8), from 9,99 E-2 to 8,80 E0 for cabbage (n=16) and from 9,94 E-3 to 1,60 E-2 for corn (n=14). It was verified that the TF values for corn presented a significant linear correlation with the following properties of the soils: exchangeable Ca and Mg, Cation Exchange Capacity and Organic matter content (p=99,9%), corroborating the behavior described in the related literature. The TF values for cabbage presented significant correlation with exchangeable Ca and pH (p=99,9%), while no correlation between TF and soil properties where observed for radish. The results of sequential extraction evidenced the great {sup 90}Sr availability for transfer in the soil-plant system; nevertheless, physiological processes occurring in such unfertile soils (low CEC, low pH and low OM and

  17. Tying the spectral ends of the M9.0 Tohoku-Oki earthquake sequence: High-frequency radiators and early quasi-static afterslip

    Science.gov (United States)

    Inbal, A.; Ampuero, J. P.; Avouac, J.; Lengliné, O.; Helmberger, D. V.

    2012-12-01

    The March 11, 2011 M9.0 Tohoku-Oki earthquake was recorded by dense seismological and geodetical networks deployed in Japan, as well as by a vast number of seismic stations worldwide. These observations allow us to study the properties of the subduction interface with unprecedented accuracy and resolution. Here we examine the spectral tails of the co- and post-seismic stages using local geodetic and seismological recordings. First, we study the details of high-frequency (HF) energy radiation during the rupture by using strong-motion recordings. Second, we jointly invert 1Hz GPS, ocean-bottom GPS and aftershock data for the spatio-temporal distribution of early afterslip. In order to constrain the spatial distribution of HF radiators we model waveform envelopes recorded by Kik-net borehole accelerometers located in northeastern Japan. We compute theoretical envelopes for waves traveling in a heterogeneous scattering medium, and invert for the location and amplitude of energy radiators for frequencies ranging from 1 to 16 Hz. Because the inversion is extremely sensitive to the response of individual sites, we adopt an empirical approach and iteratively separate the source and site terms from the stacked spectra of numerous events recorded by the network. The output response functions for each site are used to stabilize the inversion. Preliminary results are consistent with far-field observations and suggest that the HF energy emitted during the M9.0 event originated at the down-dip limit of the rupture zone. We apply waveform cross-correlation to identify repeating events within the aftershock sequence, and locate them by match-filtering their waveforms with known templates. Many of these events occur on seismic asperities loaded by the surrounding creep. We jointly invert the slip histories on these fault patches and the available GPS data for the spatio-temporal distribution of afterslip during the first few hours following the mainshock. We use the Principal

  18. Electrotransformation and expression of cellulase genes in wild-type Lactobacillus reuteri.

    Science.gov (United States)

    Li, Wang; Yang, Ming-Ming; Zhang, Guang-Qin; He, Wan-Ling; Li, Yuan-Xiao; Chen, Yu-Lin

    2012-01-01

    Two cellulase genes, Cel15 and Cel73, were amplified from Bacillus subtilis genome DNA in a previous study. Two integrative vectors, pLEM4153 and pLEM4154, containing the genes Cel15 and Cel73, respectively, were constructed and successfully electroporated into the wild-type Lactobacillus reuteri which was isolated from chick guts through an optimized procedure. Two recombinant L. reuteri were selected from a Man, Rogosa, and Sharp (MRS) plate with 10 µg/ml erythromycin, and named L. reuteri XNY-Cel15 and L. reuteri XNY-Cel73, respectively. To verify the transcription and expression of the two cellulase genes in the recombinant L. reuteri strains, the mRNA relative quantity (RQ) and the cellulase activity were determined. The mRNA RQ of Cel15 in L. reuteri XNY-Cel15 is 1,8849.5, and that of Cel73 in L. reuteri XNY-Cel73 is 1,388, and the cellulase activity of the modified MRS broth cultured with L. reuteri XNY-Cel15 was 0.158 U/ml, whereas that with L. reuteri XNY-Cel73 was 0.15 U/ml. Copyright © 2012 S. Karger AG, Basel.

  19. A method for determination of 90Sr in vegetation

    International Nuclear Information System (INIS)

    Nygren, U.

    1998-12-01

    This report describes a method for determination of 90 Sr in vegetation. The method consists of wet-ashing the samples and separating Sr from the sample matrix by oxalate precipitation and extraction chromatography. 90 Y ingrowth is awaited after which Y is separated from Sr and 90 Y measured in a proportional counter. The method has been applied on two reference materials and the 90 Sr results agree well with the recommended values. The method has also been used on 20 samples of blueberry twigs and the mean recovery of Sr was 74%

  20. Strontium 90 content in bone samples of deer and domestic animals

    International Nuclear Information System (INIS)

    Kostadinov, K.; Pozhinarova, M.

    1993-01-01

    A comparative radioecological determination of 90 Sr content in leg bone samples of wild deer from Bulgaria (Veliko Tyrnovo), rain deer from Finland (Lapland) and domestic cows and pigs from Bulgaria have been carried out. The study includes four Finish rain deer, three of them 1-5 Y old, shut in 1991, and one - 13.5 Y old, shut in 1974, two Bulgarian deer, 5-6 Y old, shut in 1991, two cows and a pig killed in 1991. The samples have been prepared by a standard procedure. The fumic nitric acid method has been used for chemical separation of 90 Sr, and a low background beta plastic scintillation device - for counting, The results show that the contamination effect is better expressed in the wild animals due to their specific open air manner of life. The 90 Sr activities vary from 41,5 to 136.9 Bq/kg bone in the Bulgarian deer, from 219.5 to 386.1 Bq/kg bone in the three younger Finish deer, and from 1921.0 to 1967.9 Bq/kg bone in the oldest rain deer. The higher 90 Sr values in the samples of the Finish deer can be connected with the specific trophic chain of these animals and the important role in it of lichen. The quantity of 90 Sr in the investigated pig is 10-20 times lower than its content in the deer (2.1 - 4.4 Bq/kg bone). It is explained with the pig's age (10 months old) and the diet based on food grown about 5 years after Chernobyl. There is no significant difference in 90 Sr content of the cows' and deer's samples. This is due to their very similar zoological systems of eating and similar trophic chains (open pasture). The data obtained show a good reproducibility proved by the similar values of the radiochemical yields achieved (60-70%) in each of the analysis made. 1 tab., 1 refs

  1. File list: His.Pan.20.AllAg.Pancreatic_cancer_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Pan.20.AllAg.Pancreatic_cancer_cells mm9 Histone Pancreas Pancreatic cancer cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Pan.20.AllAg.Pancreatic_cancer_cells.bed ...

  2. File list: His.Pan.10.AllAg.Pancreatic_cancer_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Pan.10.AllAg.Pancreatic_cancer_cells mm9 Histone Pancreas Pancreatic cancer cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Pan.10.AllAg.Pancreatic_cancer_cells.bed ...

  3. File list: His.Pan.50.AllAg.Pancreatic_cancer_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Pan.50.AllAg.Pancreatic_cancer_cells mm9 Histone Pancreas Pancreatic cancer cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/His.Pan.50.AllAg.Pancreatic_cancer_cells.bed ...

  4. Collimator and energy window optimization for 90Y bremsstrahlung SPECT imaging: A SIMIND Monte Carlo study

    International Nuclear Information System (INIS)

    Roshan, Hoda Rezaei; Mahmoudian, Babak; Gharepapagh, Esmaeil; Azarm, Ahmadreza; Pirayesh Islamian, Jalil

    2016-01-01

    Treatment efficacy of radioembolization using Yttrium-90 ( 90 Y) microspheres is assessed by the 90 Y bremsstrahlung single photon emission computed tomography (SPECT) imaging following radioembolization. The radioisotopic image has the potential of providing reliable activity map of 90 Y microspheres distribution. One of the main reasons of the poor image quality in 90 Y bremsstrahlung SPECT imaging is the continuous and broad energy spectrum of the related bremsstrahlung photons. Furthermore, collimator geometry plays an impressive role in the spatial resolution, sensitivity and image contrast. Due to the relatively poor quality of the 90 Y bremsstrahlung SPECT images, we intend to optimize the medium-energy (ME) parallel-hole collimator and energy window. The Siemens e.cam gamma camera equipped with a ME collimator and a voxelized phantom was simulated by the SImulating Medical Imaging Nuclear Detectors (SIMIND) program. We used the SIMIND Monte Carlo program to generate the 90 Y bremsstrahlung SPECT projection of the digital Jaszczak phantom. The phantom consist of the six hot spheres ranging from 9.5 to 31.8 mm in diameter, which are used to evaluate the image contrast. In order to assess the effect of the energy window on the image contrast, three energy windows ranging from 60 to 160 KeV, 160 to 400 KeV, and 60 to 400 KeV were set on a 90 Y bremsstrahlung spectrum. As well, the effect of the hole diameter of a ME collimator on the image contrast and bremsstrahlung spectrum were investigated. For the fixed collimator and septa thickness values (3.28 cm and 1.14 mm, respectively), a hole diameter range (2.35–3.3 mm) was chosen based on the appropriate balance between the spatial resolution and sensitivity. The optimal energy window for 90 Y bremsstrahlung SPECT imaging was extended energy window from 60 to 400 KeV. Besides, The optimal value of the hole diameter of ME collimator was obtained 3.3 mm. Geometry of the ME parallel-hole collimator and energy

  5. Using Cherenkov Counting For Fast Determination of 90Sr/90Y Activity in Milk

    International Nuclear Information System (INIS)

    Tsroya, S.; Dolgin, B.; German, U.; Pelled, O.; Alfassi, Z. B.

    2014-01-01

    90Sr is one of the main long-lived fission products, and it is transferred into human body primarily by food, with milk being a substantial contributor. Due to its biochemical similarity to calcium, most strontium is efficiently incorporated into bone tissues. 90Sr is characterized by a long physical half life (28.8 y) and decays by beta particles with an Emax of 0.546 MeV to 90Y. This daughter isotope has a half life of 64 h and decays into 90Zr by beta particles with an Emax of 2.284 MeV. The milk components produce a high turbidity and light attenuation, causing a significant decrease of the counting efficiency in liquid scintillation counting (LSC) systems, mostly used for beta emitters detection. Most methods proposed in the past are time-consuming, as they are based on several stages of chemical and physical treatments, including precipitation, ashing, ion exchange and extraction (Wikins et al., 1984, Porter et al, 1961, Kimura et al., 1979). When measuring 90Sr/90Y activity by Cherenkov counting, most of the Cherenkov radiation is produced by 90Y (about 98.6%), due to the much higher energy of its beta particles relative to these from 90Sr. The counting efficiency varies strongly with color quenching, at a greater extent than in standard liquid scintillation counting (L'Annunziata, 2012), and therefore the quench correction is critical. The ‘‘external source area ratio’’ (ESAR) quench correction method was applied to measure 90Sr/90Y activities in aqueous samples with a wide range of quenching levels (Tsroya et al., 2009). This method was proved to be superior to all other quench correction methods (Tsroya et al., 2012) and is applicable also for determination of 90Sr/90Y in human urine (Tsroya et al., 2013). In the present work the applicability of the ESAR method to measurement of 90Sr/90Y activities in milk and some of its products was investigated

  6. File list: Pol.Gon.05.AllAg.Testicular_germ_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.Gon.05.AllAg.Testicular_germ_cells mm9 RNA polymerase Gonad Testicular germ cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.Gon.05.AllAg.Testicular_germ_cells.bed ...

  7. Use of Chelex-100 for selectively removing Y-90 from its parent Sr-90

    International Nuclear Information System (INIS)

    Huntley, M.W.

    1996-01-01

    A method for selectively removing yttrium-90 from its parent strontium-90 contained in an environmental sample includes loading the sample onto a column containing a chelating ion-exchange resin capable of retaining yttrium-90; washing the column with a solution capable of removing strontium, calcium, and other contaminants from the yttrium-90 fraction retained on the column; removing excess acetate salts from the column; eluting yttrium-90 solution from the column and adjusting the pH of this solution to about 2.7; filtering the yttrium-90 solution and weighing this solution for gravimetric yield; and, counting the yttrium-90 containing solution with a radiological counter for a time sufficient to achieve the statistical accuracy desired. It is preferred that the chelating ion-exchange resin is a ligand having the chemical name iminodiacetic acid mounted on a divinyl benzene substrate, converted from sodium form to ammonia form

  8. Dynamic Simulation of the 2011 M9.0 Tohoku Earthquake with Geometric Complexity on a Rate- and State-dependent Subduction Plane

    Science.gov (United States)

    Luo, B.; Duan, B.

    2015-12-01

    The Mw 9.0 Tohoku megathrust earthquake on 11 March 2011 is a great surprise to the scientific community due to its unexpected occurrence on the subduction zone of Japan Trench where earthquakes of magnitude ~7 to 8 are expected based on historical records. Slip distribution and kinematic slip history inverted from seismic data, GPS and tsunami recordings reveal two major aspects of this big event: a strong asperity near the hypocenter and large slip near the trench. To investigate physical conditions of these two aspects, we perform dynamic rupture simulations on a shallow-dipping rate- and state-dependent subduction plane with topographic relief. Although existence of a subducted seamount just up-dip of the hypocenter is still an open question, high Vp anomalies [Zhao et al., 2011] and low Vp/Vs anomalies [Yamamoto et al., 2014] there strongly suggest some kind of topographic relief exists there. We explicitly incorporate a subducted seamount on the subduction surface into our models. Our preliminary results show that the subducted seamount play a significant role in dynamic rupture propagation due to the alteration of the stress state around it. We find that a subducted seamount can act as a strong barrier to many earthquakes, but its ultimate failure after some earthquake cycles results in giant earthquakes. Its failure gives rise to large stress drop, resulting in a strong asperity in slip distribution as revealed in kinematic inversions. Our preliminary results also suggest that the rate- and state- friction law plays an important role in rupture propagation of geometrically complex faults. Although rate-strengthening behavior near the trench impedes rupture propagation, an energetic rupture can break such a barrier and manage to reach the trench, resulting in significant uplift at seafloor and hence devastating tsunami to human society.

  9. Hsp90: A New Player in DNA Repair?

    Directory of Open Access Journals (Sweden)

    Rosa Pennisi

    2015-10-01

    Full Text Available Heat shock protein 90 (Hsp90 is an evolutionary conserved molecular chaperone that, together with Hsp70 and co-chaperones makes up the Hsp90 chaperone machinery, stabilizing and activating more than 200 proteins, involved in protein homeostasis (i.e., proteostasis, transcriptional regulation, chromatin remodeling, and DNA repair. Cells respond to DNA damage by activating complex DNA damage response (DDR pathways that include: (i cell cycle arrest; (ii transcriptional and post-translational activation of a subset of genes, including those associated with DNA repair; and (iii triggering of programmed cell death. The efficacy of the DDR pathways is influenced by the nuclear levels of DNA repair proteins, which are regulated by balancing between protein synthesis and degradation as well as by nuclear import and export. The inability to respond properly to either DNA damage or to DNA repair leads to genetic instability, which in turn may enhance the rate of cancer development. Multiple components of the DNA double strand breaks repair machinery, including BRCA1, BRCA2, CHK1, DNA-PKcs, FANCA, and the MRE11/RAD50/NBN complex, have been described to be client proteins of Hsp90, which acts as a regulator of the diverse DDR pathways. Inhibition of Hsp90 actions leads to the altered localization and stabilization of DDR proteins after DNA damage and may represent a cell-specific and tumor-selective radiosensibilizer. Here, the role of Hsp90-dependent molecular mechanisms involved in cancer onset and in the maintenance of the genome integrity is discussed and highlighted.

  10. Accumulation of 90Sr in sheep bones from different regions of Turkey

    International Nuclear Information System (INIS)

    Acar, R.; Okay, G.; Akman, S.

    1989-01-01

    The accumulation of 90 Sr in front leg bones of sheep which were 1-6 years old and originated from different regions of Turkey, was investigated. It was shown that 90 Sr activity strongly depends on the age of the sheep as well as on their origin. Since 90 Sr is produced during the fissioning process of uranium isotopes in nuclear reactions, the effect of radiation due to nuclear bomb experiments, nuclear reactor accidents or other radioactive pollutants in different regions of Turkey can be concluded from results obtained. (author) 6 refs.; 3 figs.; 9 tabs

  11. Worldwide deposition of 90Sr through 1984

    International Nuclear Information System (INIS)

    Larsen, R.J.; Juzdan, Z.R.

    1986-10-01

    The deposition of 90 Sr in the Northern Hemisphere during 1984 was 0.3 PBq (0.008 MCi), while that of the Southern Hemisphere was 0.1 PBq (0.003 MCi). This resulted in a total deposition on the surface of the earth during 1984 of 0.4 PBq (0.011 MCi). This is the lowest total yearly deposit since the initiation of the Environmental Measurements Laboratory's global fallout program in the mid-1950's. The worldwide cumulative deposit decreased to 357 PBq (9.6 MCi)

  12. Nepenthes Vogelii (Nepenthaceae): a new species from Sarawak

    NARCIS (Netherlands)

    Schuiteman, A.; Vogel, de E.F.

    2002-01-01

    Nepenthes vogelii Schuit. & de Vogel is described as a new species from Sarawak. It is compared with N. fusca Danser, from which it differs e.g. in the much smaller pitchers, of which the lid lacks appendages.

  13. Rapid and simultaneous determination of Strontium-89 and Strontium-90 in seawater.

    Science.gov (United States)

    Tayeb, Michelle; Dai, Xiongxin; Sdraulig, Sandra

    2016-03-01

    A rapid method has been developed for the direct determination of radiostrontium ((89)Sr and (90)Sr) released in seawater in the early phase of an accident. The method employs a fast and effective pre-concentration of radiostrontium by Sr-Ca co-precipitation followed by separation of radiostrontium using extraction chromatography technique. Radiostrontium is effectively separated in the presence of excessive dominant salts of seawater. Čerenkov and liquid scintillation assay (LSA) techniques are used to determine (89)Sr and (90)Sr. Sample preparation time is approximately 4 h for a set of 10 samples. The method was validated using spiked seawater samples at various activity ratios of (89)Sr:(90)Sr ranging from 1:10 to 9:1. The mean chemical recovery of Sr was 85 ± 3%. (90)Sr showed variable relative bias which enhanced with increasing ratio of (89)Sr:(90)Sr and was in the range ± 21%. The highest biases of (90)Sr determination were due to lower activity concentrations of (90)Sr and are regarded as acceptable in emergency situations with elevated levels of radiostrontium in the sample. The minimum detectable concentration (MDC) of (90)Sr and (89)Sr varied at different (89)Sr:(90)Sr ratios. For 0.1 L seawater and 15 min counting time on a low background Hidex liquid scintillation counter (LSC), the MDC of (90)Sr was in the range of 1.7-3.5 Bq L(-1) and MDC of (89)Sr was in the range 0.5-2.4 Bq L(-1). Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. File list: Oth.Gon.20.AllAg.Testicular_germ_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.20.AllAg.Testicular_germ_cells mm9 TFs and others Gonad Testicular germ cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Gon.20.AllAg.Testicular_germ_cells.bed ...

  15. File list: Oth.Gon.10.AllAg.Testicular_germ_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.10.AllAg.Testicular_germ_cells mm9 TFs and others Gonad Testicular germ cel...ls http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Gon.10.AllAg.Testicular_germ_cells.bed ...

  16. PRELIMINARY ESTIMATION OF POSTSEISMIC DEFORMATION PARAMETERS FROM CONTINUOUS GPS DATA IN KOREA PENINSULA AND IEODO AFTER THE 2011 TOHOKU-OKI MW9.0 EARTHQUAKE

    Directory of Open Access Journals (Sweden)

    W. A. W. Aris

    2016-09-01

    Full Text Available This paper describes utilization of GPS data in Korea Peninsula and IEODO ocean research station for investigation of postseismic deformation characteristic after the 2011 Tohoku-oki Mw9.0 Earthquake. Analytical logarithmic and exponential functions were used to evaluate the postseismic deformation parameters. The results found that the data in Korea Peninsula and IEODO during periods of mid-2011 – mid-2014 are fit better using logarithmic function with deformation decay at 134.5 ±0.1 days than using the exponential function. The result also clearly indicates that further investigation into postseismic deformation over longer data span should be taken into account to explain tectonic deformation over the region.

  17. DSM - a perspective for the 90's

    International Nuclear Information System (INIS)

    Mazzo, M.A. Jr.

    1993-01-01

    Demand Side Management (DSM) Programs are becoming important facets in the resource planning activities of many utilities. These programs have made substantial impacts to some utilities during the late 1980's, especially those in the Northeast. They will continue to play major roles in utility activities through the 90's. There are major issues which will be addressed and answered in the 90's in order for DSM to continue to play a role in the 1990's

  18. Application of a Coupled Eulerian-Lagrangian Technique on Constructability Problems of Site on Very Soft Soil

    Directory of Open Access Journals (Sweden)

    Junyoung Ko

    2017-10-01

    Full Text Available This paper presents the application of the Coupled Eulerian–Lagrangian (CEL technique on the constructability problems of site on very soft soil. The main objective of this study was to investigate the constructability and application of two ground improvement methods, such as the forced replacement method and the deep mixing method. The comparison between the results of CEL analyses and field investigations was performed to verify the CEL modelling. The behavior of very soft soil and constructability with methods can be appropriately investigated using the CEL technique, which would be useful tools for comprehensive reviews in preliminary design.

  19. Somatostatin-based radiotherapy with [90Y-DOTA]-TOC in neuroendocrine tumors: long-term outcome of a phase I dose escalation study.

    Science.gov (United States)

    Marincek, Nicolas; Jörg, Ann-Catherine; Brunner, Philippe; Schindler, Christian; Koller, Michael T; Rochlitz, Christoph; Müller-Brand, Jan; Maecke, Helmut R; Briel, Matthias; Walter, Martin A

    2013-01-15

    We describe the long-term outcome after clinical introduction and dose escalation of somatostatin receptor targeted therapy with [90Y-DOTA]-TOC in patients with metastasized neuroendocrine tumors. In a clinical phase I dose escalation study we treated patients with increasing [90Y-DOTA]-TOC activities. Multivariable Cox regression and competing risk regression were used to compare efficacy and toxicities of the different dosage protocols. Overall, 359 patients were recruited; 60 patients were enrolled for low dose (median: 2.4 GBq/cycle, range 0.9-7.8 GBq/cycle), 77 patients were enrolled for intermediate dose (median: 3.3 GBq/cycle, range: 2.0-7.4 GBq/cycle) and 222 patients were enrolled for high dose (median: 6.7 GBq/cycle, range: 3.7-8.1 GBq/cycle) [90Y-DOTA]-TOC treatment. The incidences of hematotoxicities grade 1-4 were 65.0%, 64.9% and 74.8%; the incidences of grade 4/5 kidney toxicities were 8.4%, 6.5% and 14.0%, and the median survival was 39 (range: 1-158) months, 34 (range: 1-118) months and 29 (range: 1-113) months. The high dose protocol was associated with an increased risk of kidney toxicity (Hazard Ratio: 3.12 (1.13-8.59) vs. intermediate dose, p = 0.03) and a shorter overall survival (Hazard Ratio: 2.50 (1.08-5.79) vs. low dose, p = 0.03). Increasing [90Y-DOTA]-TOC activities may be associated with increasing hematological toxicities. The dose related hematotoxicity profile of [90Y-DOTA]-TOC could facilitate tailoring [90Y-DOTA]-TOC in patients with preexisting hematotoxicities. The results of the long-term outcome suggest that fractionated [90Y-DOTA]-TOC treatment might allow to reduce renal toxicity and to improve overall survival. (ClinicalTrials.gov number NCT00978211).

  20. Large-scale purification of {sup 90}Sr from nuclear waste materials for production of {sup 90}Y, a therapeutic medical radioisotope

    Energy Technology Data Exchange (ETDEWEB)

    Wester, D.W.; Steele, R.T.; Rinehart, D.E.; DesChane, J.R.; Carson, K.J.; Rapko, B.M.; Tenforde, T.S. E-mail: tenforde@ncrp.com

    2003-07-01

    A major limitation on the supply of the short-lived medical isotope {sup 90}Y (t{sub 1/2}=64 h) is the available quantity of highly purified {sup 90}Sr generator material. A radiochemical production campaign was therefore undertaken to purify 1500 Ci of {sup 90}Sr that had been isolated from fission waste materials. A series of alkaline precipitation steps removed all detectable traces of {sup 137}Cs, alpha emitters, and uranium and transuranic elements. Technical obstacles such as the buildup of gas pressure generated upon mixing large quantities of acid with solid {sup 90}Sr carbonate were overcome through safety features incorporated into the custom-built equipment used for {sup 90}Sr purification. Methods are described for analyzing the chemical and radiochemical purity of the final product and for accurately determining by gravimetry the quantities of {sup 90}Sr immobilized on stainless steel filters for future use.

  1. Evaluation of mobility potential of 90Sr in Brazilian soils

    International Nuclear Information System (INIS)

    Viana, Aline Gonzalez

    2007-01-01

    In this document, the transfer mechanisms of 90 Sr were studied in soil-plant systems from Brazil, integrating field and laboratory experiments. Some soil classes, with different physical and chemical properties, relevant to Brazil were selected. The soil classes included in this study were Oxisol, Alfisol and Nitisol. These soils were artificially contaminated with 90 Sr for crop cultivation in lysimeters located at the experimental area of the Instituto de Radioprotecao e Dosimetria. The selected crops chosen to the root uptake studies were: corn (Zea mays, L.), cabbage (Brassica oleracea) and radish (Raphanus sativus L.). These cultures were chosen because they have varying absorption rates due to differences in the physiologic structures analyzed: grain, leaf and root. This study identified Nitisol, which contains clay of high activity, as having smaller transfer factor for all crops and these values were in the same order of magnitude as temperate climate values reported in the specialized literature: FT corn : 5,78E-3 (n=2); FT radish : 1,78 E0 (n=2); FT cabbage : 9,16E-1 (n=2). All other soils presented higher TF values, ranging from 1,93E0 to 4,06 E + 01 for radish (n=8), from 9,99 E-2 to 8,80 E0 for cabbage (n=16) and from 9,94 E-3 to 1,60 E-2 for corn (n=14). It was verified that the TF values for corn presented a significant linear correlation with the following properties of the soils: exchangeable Ca and Mg, Cation Exchange Capacity and Organic matter content (p=99,9%), corroborating the behavior described in the related literature. The TF values for cabbage presented significant correlation with exchangeable Ca and pH (p=99,9%), while no correlation between TF and soil properties where observed for radish. The results of sequential extraction evidenced the great 90 Sr availability for transfer in the soil-plant system; nevertheless, physiological processes occurring in such unfertile soils (low CEC, low pH and low OM and nutrients content) improved

  2. Determination of 90Sr in environmental samples with resonance ionization spectroscopy in collinear geometry

    International Nuclear Information System (INIS)

    Zimmer, K.; Stenner, J.; Kluge, H.J.; Lantzsch, J.; Monz, L.; Otten, E.W.; Passler, G.; Schwalbach, R.; Schwarz, M.; Stevens, H.; Wendt, K.; Herrmann, G.; Niess, S.; Trautmann, N.; Walter, K.; Bushaw, B.A.

    1994-01-01

    A new, fast technique for trace analysis of the radioactive isotopes 89 Sr and 90 Sr in environmental samples has been developed. Conventional mass separation is combined with resonance ionization spectroscopy in collinear geometry, which provides high selectivity and sensitivity. In addition, a chemical separation procedure for sample preparation has been developed. The described technique was used to determine the 90 Sr content in ∼ 870 m 3 air samples collected near Munich during and shortly after the Chernobyl reactor accident in April 1986. The content of 90 Sr was measured to be 1.4 mBq per m 3 , corresponding to 1.6 x 10 9 atoms of 90 Sr per sample. This value is in good agreement with the results of radiochemical measurements. (orig.)

  3. A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide.

    Science.gov (United States)

    Zhao, Xi-Hua; Wang, Wei; Tong, Bin; Zhang, Su-Ping; Wei, Dong-Zhi

    2016-01-01

    Compared to Trichoderma reesei RUT-C30 cellulase (Trcel), Penicillium oxalicum 16 cellulase (P16cel) from the fermentation supernatant produced a 2-fold higher glucose yield when degrading microcrystalline cellulose (MCC), possessed a 10-fold higher β-glucosidase (BGL) activity, but obtained somewhat lower other cellulase component activities. The optimal temperature and pH of β-1,4-endoglucanase, cellobiohydrolase, and filter paperase from P16cel were 50-60 °C and 4-5, respectively, but those of BGL reached 70 °C and 5. The cellulase cocktail of P16cel and Trcel had a high synergism when solubilizing MCC and generated 1.7-fold and 6.2-fold higher glucose yields than P16cel and Trcel at the same filter paperase loading, respectively. Additional low concentration of fructose enhanced the glucose yield during enzymatic hydrolysis of MCC; however, additional high concentration of monosaccharide (especially glucose) reduced cellulase activities and gave a stronger monosaccharide inhibition on Trcel. These results indicate that P16cel is a more excellent cellulase than Trcel.

  4. Environmental levels of 239+240Pu and 90Sr for internal radiation exposure assessment

    International Nuclear Information System (INIS)

    Anand, S.J.S.; Khandekar, R.N.; Krishnamoorthy, T.M.

    1995-01-01

    Measurements have been carried out on the concentration of low levels of long-lived isotopes of 239+240 Pu and 90 Sr in the environmental materials such as atmospheric particulates, drinking water and food. The estimation of daily intake of these isotopes through inhalation and ingestion is a pre-requisite for the assessment of internal exposure. This paper presents temporal distribution of 239+240 Pu and 90 Sr in rain water, drinking water and total diet samples collected at Trombay site. The annual committed effective dose due to 90 Sr through inhalation and diet to the population of Bombay has been estimated to be 0.06 nSv/y and 0.48 μSv/y, respectively, and the same for 239+240 Pu is 1.3 nSv/y and 0.9 nSv/y, respectively. The data is discussed in relation to previous years' values to assess for any significant increase. (author). 9 refs., 3 figs., 2 tabs

  5. Distribution of 90Sr and 137Cs in annual tree rings of Japanese cedar, Cryptomeria Japonica D. Don

    International Nuclear Information System (INIS)

    Chigira, Midori; Saito, Yuko; Kimura, Kan

    1988-01-01

    The contents of 90 Sr and 137 Cs in two samples of Japanese cedar from Takao and Tsukui districts were determined in tree rings cut into segments representing steps of 5 years of growth. 90 Sr in both cedar samples and 137 Cs in the Tsukui cedar sample were determined after ashing and chemical isolation, while 137 Cs in the Takao sample was directly determined from the sample ash. The distribution of 90 Sr fallout in tree rings suggests that 90 Sr had given a rather direct effect and showed no significant translocation from sapwood to heartwood, whereas 137 Cs tends to concentrate in heartwood irrespective of the effect of the fallout. Average contents of 90 Sr and 137 Cs were 22 and 9.4 pCi/kg in the Takao sample (9.61 kg air dried) and were 23 and 12 in the Tsukui (4.71 kg air dried) in 1982. (author)

  6. Long-term seismic observations along Myanmar-Sunda subduction margin: insights for 2004 M w > 9.0 earthquake

    Science.gov (United States)

    Khan, Prosanta Kumar; Banerjee, Jayashree; Shamim, Sk; Mohanty, Manoranjan

    2018-03-01

    The present study investigates the temporal variation of few seismic parameters between the Myanmar (Zone I), Andaman-Nicobar-Northwest Sumatra (Zone II), Southeast Sumatra-West Indonesia (Zone III) and East Indonesia (Zone IV) converging boundaries in reference to the generation of 26 December 2004 M w > 9.0 off-Sumatra mega-earthquake event. The four segments are distinguished based on tectonics parameters, distinct geological locations, great earthquake occurrences, and the Wadati-Benioff zone characteristics. Two important seismic parameters such as seismic energy and b values are computed over a time-window of 6-month period during the entire 1976-2013 period for these segments. The b values show a constant decrease in Zones II, III, and IV, whereas the Zone I does not show any such pattern prior to the 2004 mega-event. The release of seismic energy was also gradually decreasing in Zones II and III till the 2004 event, and little similar pattern was also noted in Zone IV. This distinct observation might be indicating that the stress accumulation was dominant near the Sumatra-Java area located towards southeast of Zone II and northwest of Zone III. The released strain energy during the 2004 event was subsequently migrated towards north, rupturing 1300 km of the boundary between the Northwest Sumatra and the North Andaman. The occurrence of 2004 mega-event was apparently concealed behind the long-term seismic quiescence existing near the Sumatra and Nicobar margin. A systematic study of the patterns of seismic energy release and b values, and the long-term observation of collective behaviour of the margin tectonics might have had given clues to the possibility of the 2004 mega-event.

  7. Determination of Sr90 activity in human bones

    International Nuclear Information System (INIS)

    Mendonca, Anamelia Habib

    1970-01-01

    significant difference between mean values of the three age groups studied. However when we consider only 5 samples, from 18 months to 9 years old, the average is significantly higher than the ones of older ages. These results seem to indicate that a more thorough study should be performed where levels of Sr 90 in human bones and in the constituents of the Brazilian diet could be related. (author)

  8. Anti-citrullinated heat shock protein 90 antibodies identified in bronchoalveolar lavage fluid are a marker of lung-specific immune responses.

    Science.gov (United States)

    Harlow, Lisa; Gochuico, Bernadette R; Rosas, Ivan O; Doyle, Tracy J; Osorio, Juan C; Travers, Timothy S; Camacho, Carlos C; Oddis, Chester V; Ascherman, Dana P

    2014-11-01

    Previous work has demonstrated a correlation between serum anti-citrullinated HSP90 antibodies and rheumatoid arthritis-associated interstitial lung disease (RA-ILD). To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. 9/21 RA-derived BALF specimens demonstrated IgG and/or IgA antibodies targeting citrullinated HSP90 proteins/peptides, highlighting disease specific responses (with a predilection for RA-ILD) that did not occur in IPF patients (0/5) or healthy control subjects (0/5). Comparison of antibody profiles between BALF and matching serum specimens revealed various recognition patterns favoring predominant production of anti-citrullinated HSP90 antibodies within the lung microenvironment-further supporting the connection between this antibody specificity and parenchymal lung disease. Equally important, qualitative as well as quantitative differences in anti-citrullinated HSP90 profiles between BALF and serum indicate that the lung plays a direct role in shaping the immune repertoire of RA/RA-ILD. Published by Elsevier Inc.

  9. Composição química do capim-elefante cv. Roxo cortado em diferentes alturas Chemical composition of elephant grass var. Roxo cut at different heights

    Directory of Open Access Journals (Sweden)

    Estácio Alves dos Santos

    2001-02-01

    Full Text Available O objetivo deste trabalho foi verificar o efeito dos cortes efetuados a 0, 15, 30 e 45 cm sobre a composição química do capim-elefante (Pennisetum purpureum, Schum. cv. Roxo, em épocas seca e chuvosa. As amostras foram obtidas de uma área útil de 8,4 m² de cada parcela. Após o corte de uniformização, efetuaram-se dois cortes no período seco, em intervalos de 90 dias, e três no período chuvoso, em intervalos de 60 dias. De cada parcela foi tomada uma amostra de 3 a 5 perfilhos, desidratada em estufa e triturada para analises laboratoriais. Foram avaliados os percentuais de matéria seca (MS, proteína bruta (PB, fibra em detergente neutro (FDN, fibra em detergente ácido (FDA, celulose (CEL, hemicelulose (HCEL, lignina (LIG e cinzas. As alturas de corte não influenciaram a composição química da forrageira, nem houve interação com as épocas. Com exceção de hemicelulose e cinzas, os cortes na época seca mostraram resultados superiores à chuvosa. As médias nas duas épocas foram 19,70 e 17,44% para MS; 7,74 e 7,25% para PB; 76,41 e 71,13% para FDN; 42,75 e 41,02% para FDA; 31,44 e 30,43% para CEL; 30,66 e 30,28% para HCEL; 9,25 e 7,83% para LIG; e 1,97 e 3,38% para cinzas, nos períodos seco e chuvoso, respectivamente.This work was carried out to evaluate the cutting effect at 0, 15, 30 and 45 cm height, on the chemical composition of elephant grass (Pennisetum purpureum, Schum. cv. Roxo in dry and rainy seasons. The samples were obtained from an useful area of 8,4 m² from each parcel. After the uniformity cut two cuts in dry season with 90 days interval, and three cuts in rainy seasons with 60 days interval. A sample of 3 or 5 tillers was taken from each parcel and they were dehydrated in air forced stove and ground for laboratorial analysis. The percentages of dry matter (DM, crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF, cellulose (CEL, hemicellulose (HCEL, lignin (LIG, and ashes were

  10. High-resolution blood-pool-contrast-enhanced MR angiography in glioblastoma: tumor-associated neovascularization as a biomarker for patient survival. A preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Puig, Josep; Blasco, Gerard; Remollo, Sebastian; Hernandez, David; Pedraza, Salvador [Hospital Universitari Dr Josep Trueta, Research Unit of Diagnostic Imaging Institute (IDI), Department of Radiology [Girona Biomedical Research Institute] IDIBGI, Girona (Spain); Daunis-i-Estadella, Josep; Mateu, Gloria [University of Girona, Department of Computer Science, Applied Mathematics and Statistics, Girona (Spain); Alberich-Bayarri, Angel [La Fe Polytechnics and University Hospital, Biomedical Imaging Research Group (GIBI230), La Fe Health Research Institute, Valencia (Spain); Essig, Marco [University of Manitoba, Department of Radiology, Winnipeg (Canada); Jain, Rajan [NYU School of Medicine, Division of Neuroradiology, Department of Radiology, New York, NY (United States); Puigdemont, Montserrat [Hospital Universitari Dr Josep Trueta, Catalan Institute of Oncology (ICO), Hospital Cancer Registry, Girona (Spain); Sanchez-Gonzalez, Javier [Philips Healthcare Iberica, Madrid (Spain); Wintermark, Max [Stanford University, Department of Radiology, Neuroradiology Division, Palo Alto, CA (United States)

    2016-01-15

    The objective of the study was to determine whether tumor-associated neovascularization on high-resolution gadofosveset-enhanced magnetic resonance angiography (MRA) is a useful biomarker for predicting survival in patients with newly diagnosed glioblastomas. Before treatment, 35 patients (25 men; mean age, 64 ± 14 years) with glioblastoma underwent MRI including first-pass dynamic susceptibility contrast (DSC) perfusion and post-contrast T1WI sequences with gadobutrol (0.1 mmol/kg) and, 48 h later, high-resolution MRA with gadofosveset (0.03 mmol/kg). Volumes of interest for contrast-enhancing lesion (CEL), non-CEL, and contralateral normal-appearing white matter were obtained, and DSC perfusion and DWI parameters were evaluated. Prognostic factors were assessed by Kaplan-Meier survival and Cox proportional hazards model. Eighteen (51.42 %) glioblastomas were hypervascular on high-resolution MRA. Hypervascular glioblastomas were associated with higher CEL volume and lower Karnofsky score. Median survival rates for patients with hypovascular and hypervascular glioblastomas treated with surgery, radiotherapy, and chemotherapy were 15 and 9.75 months, respectively (P < 0.001). Tumor-associated neovascularization was the best predictor of survival at 5.25 months (AUC = 0.794, 81.2 % sensitivity, 77.8 % specificity, 76.5 % positive predictive value, 82.4 % negative predictive value) and yielded the highest hazard ratio (P < 0.001). Tumor-associated neovascularization detected on high-resolution blood-pool-contrast-enhanced MRA of newly diagnosed glioblastoma seems to be a useful biomarker that correlates with worse survival. (orig.)

  11. Hsp90 depletion goes wild

    OpenAIRE

    Siegal, Mark L; Masel, Joanna

    2012-01-01

    Abstract Hsp90 reveals phenotypic variation in the laboratory, but is Hsp90 depletion important in the wild? Recent work from Chen and Wagner in BMC Evolutionary Biology has discovered a naturally occurring Drosophila allele that downregulates Hsp90, creating sensitivity to cryptic genetic variation. Laboratory studies suggest that the exact magnitude of Hsp90 downregulation is important. Extreme Hsp90 depletion might reactivate transposable elements and/or induce aneuploidy, in addition to r...

  12. High Frequency Infrasonic Radiation from the 11 March 2011 Tohoku Mw 9.0 Earthquake

    Science.gov (United States)

    Walker, K. T.; Le Pichon, A.; Degroot-Hedlin, C. D.; Che, I.

    2011-12-01

    The tragic March 11 Mw 9.0 Tohoku earthquake ruptured the Wadati-Benioff zone beneath northeast Japan, generating a damaging seismic wavetrain and triggering a tsunami that devastated the nearby coastal areas. Centroid moment tensors, aftershocks, and the geometry of the trench suggest the rupture occurred on a plane roughly 400 km long by 200 km wide. Because the Earth's surface is effectively a speaker, the seismic wavetrain generated infrasonic emissions from northeast Japan that were recorded by seven infrasound arrays within 5600 km of the epicenter. Using a time progressive beamforming method and the Progressive Multi-Channel Correlation method, we detect and calculate back azimuths for the 0.3 to 3 Hz infrasonic signals at these stations. After application of predicted wind corrections, these back azimuths point to Honshu and Hokkaido, with the majority of detections illuminating a north-south elongated area near Sendai, where the USGS ShakeMap predicts the greatest intensity of surface shaking. An array near Tokyo (IS30) provides the first recording of locally generated infrasound from a very large dip-slip earthquake. At IS30 a six-minute arrival in the 0.3 to 1.5 Hz band is observed from northeast Japan spanning an 18° back azimuth range. Two shorter events originate from a source to the west, likely Mt. Fuji. Using constraints from propagation modeling, we back project the infrasonic amplitudes recorded at IS30 to a relatively localized area. The maximum amplitude of 1 Pa originates from surface shaking along the coast. This location is also just west of the epicenter and adjacent to the location of maximum P-wave radiation from back projection studies. Noise at IS30 after the mainshock limits the detection of additional signals. A more pronounced infrasonic wavetrain at IS44 (Kamchatka) illuminates the entire Honshu and Hokkaido region, especially along the east coast near Sendai. In agreement with propagation modeling predictions using global

  13. Habitat, density and group size of primates in a Brazilian tropical forest.

    Science.gov (United States)

    Pinto, L P; Costa, C M; Strier, K B; da Fonseca, G A

    1993-01-01

    Habitats, population densities and group sizes of 5 primate species (Callithrix flaviceps, Callicebus personatus personatus, Cebus apella nigritus, Alouatta fusca clamitans, and Brachyteles arachnoides) were estimated, using the method of repeated transect sampling, in an area of montane pluvial forest in eastern Brazil (Atlantic forest). A. fusca and C. apella had the highest densities in terms of groups and individuals per square kilometer, respectively, while B. arachnoides was least abundant. The highest primate densities were observed in areas of secondary vegetation. Both group sizes and population densities for the 5 species were generally lower at the Reserva Biologica Augusto Ruschi than those reported in other areas of Atlantic forest. Hunting pressure and the different carrying capacity of the habitat are suggested as possible causes for the low number of sightings registered for these species.

  14. JTpack90: A parallel, object-based, Fortran 90 linear algebra package

    Energy Technology Data Exchange (ETDEWEB)

    Turner, J.A.; Kothe, D.B. [Los Alamos National Lab., NM (United States); Ferrell, R.C. [Cambridge Power Computing Associates, Ltd., Brookline, MA (United States)

    1997-03-01

    The authors have developed an object-based linear algebra package, currently with emphasis on sparse Krylov methods, driven primarily by needs of the Los Alamos National Laboratory parallel unstructured-mesh casting simulation tool Telluride. Support for a number of sparse storage formats, methods, and preconditioners have been implemented, driven primarily by application needs. They describe the object-based Fortran 90 approach, which enhances maintainability, performance, and extensibility, the parallelization approach using a new portable gather/scatter library (PGSLib), current capabilities and future plans, and present preliminary performance results on a variety of platforms.

  15. File list: Pol.ALL.20.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.20.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III All cel...l types ERX204069 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.20.RNA_Polymerase_III.AllCell.bed ...

  16. File list: Pol.ALL.50.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pol.ALL.50.RNA_Polymerase_III.AllCell mm9 RNA polymerase RNA Polymerase III All cel...l types ERX204069 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Pol.ALL.50.RNA_Polymerase_III.AllCell.bed ...

  17. File list: DNS.Neu.50.AllAg.Neural_progenitor_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Neu.50.AllAg.Neural_progenitor_cells mm9 DNase-seq Neural Neural progenitor cel...ls SRX238870,SRX238868 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Neu.50.AllAg.Neural_progenitor_cells.bed ...

  18. File list: DNS.Neu.05.AllAg.Neural_progenitor_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Neu.05.AllAg.Neural_progenitor_cells mm9 DNase-seq Neural Neural progenitor cel...ls SRX238870,SRX238868 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Neu.05.AllAg.Neural_progenitor_cells.bed ...

  19. File list: DNS.Neu.10.AllAg.Neural_progenitor_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Neu.10.AllAg.Neural_progenitor_cells mm9 DNase-seq Neural Neural progenitor cel...ls SRX238868,SRX238870 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.Neu.10.AllAg.Neural_progenitor_cells.bed ...

  20. 40 CFR 90.206 - Trading.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Trading. 90.206 Section 90.206... Trading Provisions § 90.206 Trading. (a) An engine manufacturer may exchange emission credits with other engine manufacturers in trading, subject to the trading restriction specified in § 90.207(c)(2). (b...

  1. Y-90 microsphere therapy: prevention of adverse events.

    Science.gov (United States)

    Schultz, Cheryl C; Campbell, Janice; Bakalyar, Donovan; Beauvais, Michele; Feng, Wenzheng; Savin, Michael

    2009-08-01

    Thirty-three (33) events that were inconsistent with intended treatment for 471 Y-90 microsphere deliveries were analyzed from 2001 to 2007. Each occurrence was categorized, based on root-cause analysis, as a device/product defect and/or operator error event. Events were further categorized, if there was an adverse outcome, as spill/leak, termination, recatheterization, dose deviation, and/or a regulatory medical event. Of 264 Y-90 Therasphere (MDS Nordion, Ottawa, Ontario, Canada) treatments, 15 events were reported (5.7%). Of 207 Y-90 SIR-Spheres (Sirtex, Wilmington, MA) treatments, 18 events were reported (8.7%). Twenty-five (25) of 33 events (76%) were device/product defects: 73% for Therasphere (11 of 15) and 78% for SIR-Spheres (14 of 18). There were 31 adverse outcomes associated with 33 events: 15 were leaks and/or spills, 9 resulted in termination of the dose administration, 3 resulted in recatheterization for dose compensation, 2 were dose deviations (doses differing from the prescribed between 10% and 20%), and 2 were reported as regulatory medical events. Fifty-five (55) corrective actions were taken: 39 (71%) were related to the manufacturer and 16 (29%) were hospital based. This process of analyzing each event and measuring our outcomes has been effective at minimizing adverse events and improving patient safety.

  2. Time-dependent earthquake probability calculations for southern Kanto after the 2011 M9.0 Tohoku earthquake

    Science.gov (United States)

    Nanjo, K. Z.; Sakai, S.; Kato, A.; Tsuruoka, H.; Hirata, N.

    2013-05-01

    Seismicity in southern Kanto activated with the 2011 March 11 Tohoku earthquake of magnitude M9.0, but does this cause a significant difference in the probability of more earthquakes at the present or in the To? future answer this question, we examine the effect of a change in the seismicity rate on the probability of earthquakes. Our data set is from the Japan Meteorological Agency earthquake catalogue, downloaded on 2012 May 30. Our approach is based on time-dependent earthquake probabilistic calculations, often used for aftershock hazard assessment, and are based on two statistical laws: the Gutenberg-Richter (GR) frequency-magnitude law and the Omori-Utsu (OU) aftershock-decay law. We first confirm that the seismicity following a quake of M4 or larger is well modelled by the GR law with b ˜ 1. Then, there is good agreement with the OU law with p ˜ 0.5, which indicates that the slow decay was notably significant. Based on these results, we then calculate the most probable estimates of future M6-7-class events for various periods, all with a starting date of 2012 May 30. The estimates are higher than pre-quake levels if we consider a period of 3-yr duration or shorter. However, for statistics-based forecasting such as this, errors that arise from parameter estimation must be considered. Taking into account the contribution of these errors to the probability calculations, we conclude that any increase in the probability of earthquakes is insignificant. Although we try to avoid overstating the change in probability, our observations combined with results from previous studies support the likelihood that afterslip (fault creep) in southern Kanto will slowly relax a stress step caused by the Tohoku earthquake. This afterslip in turn reminds us of the potential for stress redistribution to the surrounding regions. We note the importance of varying hazards not only in time but also in space to improve the probabilistic seismic hazard assessment for southern Kanto.

  3. Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate

    Directory of Open Access Journals (Sweden)

    Petrović Đorđe Ž.

    2012-01-01

    Full Text Available Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA0,Tyr3] octreotate and the preparation of [90Y-DOTA0,Tyr3] octreotate (90Y-DOTATATE for peptide receptore radionuclide therapy. 90Sr/90Y generator was based on the electrochemical separation of 90Y from 90Sr in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionuclidic purity of the 90Y solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mg DOTATATE with 90YCl3 was performed at 95°C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for 90Y. The achieved efficiency of the 90Sr/90Y generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with 90Y exhibited a high efficiency, too: there was less than 1% of 90Y3+in the 90Y-DOTATATE.

  4. An Overview of the Production, Quality Control and Feasibility of Using 90Y as a Therapeutic Radionuclide

    International Nuclear Information System (INIS)

    Venkatesh, M.

    2009-01-01

    90 Y is increasingly accepted world wide as a radionuclide for in-vivo therapy owing to attractive decay features (T1/2 2.67 d; Eβ max 2.28 MeV) and viable production feasibility in high specific activities. 90 Y is most often recommended for treatment of large tumour lesions as the hard β rays are effective in delivering therapeutic dose to large volume. However, possibility of high radiation dose to the critical organs such as bone marrow and kidneys is an important concern that is given due weightage while designing therapy using 90 Y. The best route to avail 90 Y for therapy applications is from 90 Sr, though neutron actiation of natural 89 Y(100% abundance) is feasible. The absorption cross section σ is barely 1.38 b, resulting in low specific activity 90 Y which is useful for limited applications. The possibility of obtaining 90Y through a radionuclide generator as the daughter of a long lived parent 90 Sr (T1/2 28.9 y) is a major advantage that enables access to high specific activity 90 Y. Transporting the 90Y activity to a user institution from a centralized production facility is reasonably feasible and this facilitates its wide spread use. Several generator designs have been developed and reported to access 90 Y. Solvent extraction using a chelating molecule in an organic solvent (0.3M HDEHP/n-dodecane), column chromatography using ion exchange resins (cationic as well as anionic; Dowex-50x8; AG 50x16; Aminex-A5) or inorganic exchanger, membrane based separation using chelating ligand impregnated membranes (CMPO in electrochemical separation are some of the methods reported. Limitations such as elution of 90 Y after initial elution of 90 Sr, availability of 90 Y as a chelated complex which then has to be treated to enable labeling the molecule of interest, possibility of obtaining small quantities of 90 Y owing to radiolytic damages to the separation system components, paucity of special automation gadgets for handling the high activities remotely

  5. Identification of the First Receptor for a Pregnancy Specific Glycoprotein. Tetraspanins Find Their Ligand

    Science.gov (United States)

    2001-04-13

    across the placenta, resulting in protective immunity for the neonate before the endogenous immune system develops" [127]. The precise in vivo...St rcp -FITC • • • ~lUOl"’f!S«!IlY Im.",oi,,’ ’’> Figure 3. FACS analysis of PSG17N-Myc-His binding to CD9 transfected 293T cells. (A) 293T cel ls

  6. Effect of irradiation on Nε-carboxymethyl-lysine and Nε-carboxyethyl-lysine formation in cooked meat products during storage

    International Nuclear Information System (INIS)

    Yu, Ligang; He, Zhiyong; Zeng, Maomao; Zheng, Zongping; Chen, Jie

    2016-01-01

    This study investigated the effects of irradiation on N ε -carboxymethyl-lysine (CML) and N ε -carboxyethyl-lysine (CEL) formation in cooked red and white meats during storage. The results showed that irradiation did not affect CML/CEL formation (0 weeks). After 6 weeks, CML/CEL contents in the irradiated samples exhibited a higher growth rate than the non-irradiated samples, especially the red meat. The results of electron spin resonance spectrometry and 2-Thiobarbituric acid-reactive substances suggested irradiation had induced free-radical reactions and accelerated lipid oxidation during storage. A linear correlation (r=0.810–0.906, p<0.01) was found between the loss of polyunsaturated fatty acids content and increase of CML/CEL content in the irradiated samples after 0 and 6 weeks of storage. The results indicate that irradiation-induced lipid oxidation promotes CML/CEL formation, and CML/CEL formation by the lipid oxidation pathways may be an important pathway for CML/CEL accumulation in irradiated meat products during storage. - Highlights: • The effect of irradiation on CML and CEL formation in meat products is investigated. • CML and CEL contents in irradiated meat products exhibit a higher growth rate than non-irradiated samples. • PUFAs oxidation induced by irradiation promotes CML and CEL formation. • Lipid oxidation pathways are an important pathway for CML and CEL accumulation in irradiated samples during storage.

  7. Toxicity of 90Sr inhaled in a relatively insoluble form by Beagle dogs. IX

    International Nuclear Information System (INIS)

    Snipes, M.B.; Hahn, F.F.; Muggenburg, B.A.; Mauderly, J.L.; McClellan, R.O.; Pickrell, J.A.

    1978-01-01

    Studies on the biological effects of 90 Sr inhaled in a relatively insoluble form by Beagle dogs are being conducted to define the biological consequences of inhaling this radionuclide in a form which has a long retention time in the lung. One hundred and six dogs were exposed to a polydisperse aerosol (AMAD 1.4 to 2.8 μm and sigma/sub g/ 1.4 to 2.7) of fused aluminosilicate particles labeled with 90 Sr. Initial lung burdens ranged from 0.21 to 94 μCi 90 Sr per kilogram of body weight (μCi/kg). Eighteen control dogs were exposed to an aerosol of stable strontium in fused aluminosilicate particles. To date, 34 dogs having initial lung burdens of 8.9 to 94 μCi 90 Sr/kg and cumulative doses to lung of 33,000 to 96,000 rads have died from radiation pneumonitis and/or pulmonary fibrosis between 159 and 2596 days after exposure. Twenty-nine dogs with initial lung burdens of 3.7 to 36 μCi 90 Sr/kg and cumulative doses to lung of 13,000 to 68,000 rads have died from hemangiosarcomas in the lung, heart or mediastinum between 644 and 2565 days after exposure. In addition, one dog developed a bronchioloalveolar carcinoma, another developed epidermoid carcinoma of the lung, another died of pneumonia while recovering from anesthesia, one dog died at 1821 days after exposure with an hemangiosarcoma of the spleen and two dogs developed squamous cell carcinomas in the nasal cavity. During the past year one dog was euthanized 2753 days after exposure (34,000 rads lung dose) as a result of an hemangiosarcoma which developed in a rib and spread widely. Another dog died 2830 days after exposure (35,000 rads lung dose) with a squamous cell carcinoma in the lung. An additional dog was euthanized 2636 days after exposure (12,000 rads lung dose) with widespread hemangiosarcoma of unknown origin. The remaining 36 exposed dogs and 18 controls are surviving at 1387 to 3168 days after exposure

  8. Responses of two tall buildings in Tokyo, Japan, before, during, and after the M9.0 Tohoku earthquake of 11 March 2011

    Science.gov (United States)

    Çelebi, Mehmet; Hisada, Yoshiuaki; Omrani, Roshanak; Ghahari, S. Farid; Taciroglu, Ertugrul

    2016-01-01

    The 11 March 2011 M 9.0 Tohoku earthquake generated significant long duration shaking that propagated hundreds of kilometers from the epicenter and affected urban areas throughout much of Honshu. Recorded responses of tall buildings at several hundred km from the epicenter of the main shock and other events show tall buildings were affected by long-period motions of events at distant sources. This study presents behavioral aspects of 29-story and 30-story neighboring buildings in the Shinjuku area of Tokyo, Japan, as inferred from records retrieved from a sparse array of accelerometers deployed in the superstructures, at ground and 100 m below the ground level over a time interval covering before, during, and after the main shock. Such long-period effects are common in several regions of Japan as well as in the United States and in other seismically active countries. Permanent shifts in fundamental frequencies are observed. Drift ratios indicate possible structural nonlinear behavior occurred during the main shock. The need to consider risks to built environments from distant sources, including those in neighboring countries, is emphasized.

  9. A data acquisition system for indentification of 90Sr/ 90Y in environmental samples

    International Nuclear Information System (INIS)

    Medin, G.; Brajnik, D.; Starcic, M.; Stanovnik, A.

    1996-01-01

    Often, in the stage of research and development of new techniques for detection of ionizing radiation, elaborate electronic systems are required. In this paper, we describe the relatively complex detector and electronic system used for a relatively simple but nevertheless demanding measurement of the beta emitting radionuclides 90 Sr/ 90 Y in environmental samples. The detection limit of 1 Bq in a thin, disc-shaped sample, was obtained by careful elimination of background. Contribution of other radionuclides in the sample, were eliminated or at least considerably reduced by using a silica aerogel as Cherenkov radiator and thin multiwire chamber in coincidence. Cosmic ray signals were reduced by large scintillation counters in anticoincidence. Finally, persisting RF pick-up signals were eliminated by using the signal from a wire antenna and identical MWPC preamplifier and discriminator for a veto to the master coincidence. For each accepted event, both timing and pulse height information was recorded with a personal computer

  10. Intracellular dynamics of the Hsp90 co-chaperone p23 is dictated by Hsp90

    International Nuclear Information System (INIS)

    Picard, Didier

    2006-01-01

    p23 is a component of the Hsp90 molecular chaperone machine. It binds and stabilizes the ATP-bound dimeric form of Hsp90. Since Hsp90 binds protein substrates in the ATP conformation, p23 has been proposed to stabilize Hsp90-substrate complexes. In addition, p23 can also function as a molecular chaperone by itself and even possesses an unrelated enzymatic activity. Whether it fulfills the latter functions in cells while bound to Hsp90 remains unknown and is difficult to extrapolate from cell-free biochemical experiments. Using the 'fluorescence recovery after photobleaching' (FRAP) technology, I have examined the dynamics of human p23, expressed as a fusion protein with the green fluorescent protein (GFP), in living human HeLa cells. GFP-p23 is distributed throughout the cell, and its mobility is identical in the cytoplasm and in the nucleus. When the Hsp90 interaction is disrupted either with the Hsp90 inhibitor geldanamycin or by introduction of point mutations into p23, the mobility of p23 is greatly accelerated. Under these conditions, its intracellular movement may be diffusion-controlled. In contrast, when wild-type p23 is able to bind Hsp90, a more complex FRAP behavior is observed, suggesting that it is quantitatively bound in Hsp90 complexes undergoing a multitude of other interactions

  11. Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China.

    Science.gov (United States)

    Wu, Xiaodong; Wu, Xiaoyun; Li, Wenbin; Cheng, Xiaofei

    2018-05-01

    Through sequencing and assembly of small RNAs, an orthotospovirus was identified from a celtuce plant (Lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the Zhejiang province of China. The S, M, and L RNAs of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. The full length nucleoprotein (N) of this orthotospovirus shared highest amino acid sequence identity (90.25%) with that of calla lily chlorotic spot virus isolated from calla lily (CCSV-calla) [China: Taiwan: 2001] and tobacco (CCSV-LJ1) [China: Lijiang: 2014]. Phylogenetic analyses showed that this orthotospovirus is phylogenetically associated with CCSV isolates and clustered with CCSV, tomato zonate spot virus (TZSV), and tomato necrotic spot-associated virus (TNSaV) in a separate sub-branch. These results suggest that this orthotospovirus is a divergent isolate of CCSV and was thus named CCSV-Cel [China: Zhejiang: 2017].

  12. Depth dose distribution in the water for clinical applicators of 90Sr + 90Y, with a extrapolation mini chamber

    International Nuclear Information System (INIS)

    Antonio, Patricia de Lara; Caldas, Linda V.E.; Oliveira, Mercia L.

    2009-01-01

    This work determines the depth dose in the water for clinical applicators of 90 Sr + 90 Y, using a extrapolation mini chamber developed at the IPEN, Sao Paulo, Brazil, and different thickness acrylic plates. The obtained results were compared with the international recommendations and were considered satisfactory

  13. Hsp90 depletion goes wild

    Directory of Open Access Journals (Sweden)

    Siegal Mark L

    2012-02-01

    Full Text Available Abstract Hsp90 reveals phenotypic variation in the laboratory, but is Hsp90 depletion important in the wild? Recent work from Chen and Wagner in BMC Evolutionary Biology has discovered a naturally occurring Drosophila allele that downregulates Hsp90, creating sensitivity to cryptic genetic variation. Laboratory studies suggest that the exact magnitude of Hsp90 downregulation is important. Extreme Hsp90 depletion might reactivate transposable elements and/or induce aneuploidy, in addition to revealing cryptic genetic variation. See research article http://wwww.biomedcentral.com/1471-2148/12/25

  14. Determination of 90Sr in milk by solvent extraction of 90Y

    International Nuclear Information System (INIS)

    Mitsuhashi, Toshihiko; Sakanoue, Masanobu.

    1977-01-01

    In order to replace the conventional method using violent fuming nitric acid, a new method for the determination of 90 Sr in milk has been developed by using the solvent extraction with bis (2-ethylhexyl) phosphoric acid (HDEHP). The daughter nuclide 90 Y in a radiochemical equilibrium with its parent 90 Sr was extracted with 2:1 HDEHP-toluene from the acid solution (1M HCl) of milk ash sample prepared by dry-ashing. After stripping with 8M HCl, 90 Y, together with stable yttrium added as carrier, was precipitated as oxalate to prepare β-counting source. The radiochemical purity was confirmed by decay curve. The decontamination of strontium was checked by applying non-dispersive fluorescence x-ray analysis using 133 Ba as irradiating source. Bone samples of cow were also analyzed by the same method and the results were compared with those obtained by other methods. The duplicate crosschecking analyses of finely ground bone samples were carried out to examine the effectiveness of this method. This simple new method was found to be very effective for the routine analysis of 90 Sr in these samples. (auth.)

  15. BWR 90 and BWR 90+: Two advanced BWR design generations from ABB

    International Nuclear Information System (INIS)

    Haukeland, S.; Ivung, B.; Pedersen, T.

    1999-01-01

    ABB has two evolutionary advanced light water reactors available today - the BWR 90 boiling water reactor and the System 80+ pressurised water reactor. The BWR 90 is based on the design, construction, commissioning and operation of the BWR 75 plants. The operation experience of the six plants of this advanced design has been very good. The average annual energy availability is above 90%, and total power generation costs have been low. When developing the BWR 90 specific changes were introduced to a reference design, to adapt to technological progress, new safety requirements and to achieve cost savings. The thermal power rating of BWR 90 is 3800 MWth (providing a nominal 1374 MWe net), slightly higher than that of the reference plant ABB Atom has taken advantage of margins gained using a new generation of its SVEA fuel to attain this power rating without major design modifications. The BWR 90 design was completed and offered to the TVO utility in Finland in 1991, as one of the contenders for the fifth Finnish nuclear power plant project. Hence, the design is available today for deployment in new plant projects. Utility views were incorporated through co-operation with the Finnish utility TVO, owner and operator of the two Olkiluoto plants of BWR 75 design. A review against the European Utility Requirement (EUR) set of requirements has been performed, since the design, in 1997, was selected by the EUR Steering Committee to be the first BWR to be evaluated against the EUR documents. The review work was completed in 1998. It will be the subject of an 'EUR Volume 3 Subset for BWR 90' document. ABB is continuing its BWR development work with an 'evolutionary' design called BWR 90+, which aims at developing the BWR as a competitive option for the anticipated revival of the market for new nuclear plants beyond the turn of the century, as well as feeding ideas and inputs to the continuous modernisation efforts at operating plants. The development is performed by ABB Atom

  16. Anti-CD45 radioimmunotherapy with 90Y but not 177Lu is effective treatment in a syngeneic murine leukemia model.

    Directory of Open Access Journals (Sweden)

    Johnnie J Orozco

    Full Text Available Radioimmunotherapy (RIT for treatment of hematologic malignancies has primarily employed monoclonal antibodies (Ab labeled with 131I or 90Y which have limitations, and alternative radionuclides are needed to facilitate wider adoption of RIT. We therefore compared the relative therapeutic efficacy and toxicity of anti-CD45 RIT employing 90Y and 177Lu in a syngeneic, disseminated murine myeloid leukemia (B6SJLF1/J model. Biodistribution studies showed that both 90Y- and 177Lu-anti-murine CD45 Ab conjugates (DOTA-30F11 targeted hematologic tissues, as at 24 hours 48.8 ± 21.2 and 156 ± 14.6% injected dose per gram of tissue (% ID/g of 90Y-DOTA-30F11 and 54.2 ± 9.5 and 199 ± 11.7% ID/g of 177Lu-DOTA-30F11 accumulated in bone marrow (BM and spleen, respectively. However, 90Y-DOTA-30F11 RIT demonstrated a dose-dependent survival benefit: 60% of mice treated with 300 µCi 90Y-DOTA-30F11 lived over 180 days after therapy, and mice treated with 100 µCi 90Y-DOTA-30F11 had a median survival 66 days. 90Y-anti-CD45 RIT was associated with transient, mild myelotoxicity without hepatic or renal toxicity. Conversely, 177Lu- anti-CD45 RIT yielded no long-term survivors. Thus, 90Y was more effective than 177Lu for anti-CD45 RIT of AML in this murine leukemia model.

  17. Characterization of an extrapolation chamber in a 90Sr/90Y beta radiation field

    International Nuclear Information System (INIS)

    Oramas Polo, I.; Tamayo Garcia, J. A.

    2015-01-01

    The extrapolation chamber is a parallel plate chamber and variable volume based on the Bragg-Gray theory. It determines in absolute mode, with high accuracy the dose absorbed by the extrapolation of the ionization current measured for a null distance between the electrodes. This camera is used for dosimetry of external beta rays for radiation protection. This paper presents the characterization of an extrapolation chamber in a 90 Sr/ 90 Y beta radiation field. The absorbed dose rate to tissue at a depth of 0.07 mm was calculated and is (0.13206±0.0028) μGy. The extrapolation chamber null depth was determined and its value is 60 μm. The influence of temperature, pressure and humidity on the value of the corrected current was also evaluated. Temperature is the parameter that has more influence on this value and the influence of pressure and the humidity is not very significant. Extrapolation curves were obtained. (Author)

  18. Nuclear transparency in 90 deg.c.m. quasielastic A(p,2p) reactions

    International Nuclear Information System (INIS)

    Aclander, J.; Alster, J.; Kosonovsky, I.; Malki, A.; Mardor, I.; Mardor, Y.; Navon, I.; Piasetzky, E.; Asryan, G.; Barton, D.S.; Buktoyarova, N.; Bunce, G.; Carroll, A.S.; Gushue, S.; Makdisi, Y.I.; Roser, T.; Tanaka, M.; Averiche, Y.; Panebratsev, Y.; Shimanskiy, S.

    2004-01-01

    We summarize the results of two experimental programs at the Alternating Gradient Synchrotron of BNL to measure the nuclear transparency of nuclei measured in the A(p,2p) quasielastic scattering process near 90 deg. in the pp center of mass. The incident momenta varied from 5.9 to 14.4 GeV/c, corresponding to 4.8 2 2 . Taking into account the motion of the target proton in the nucleus, the effective incident momenta extended from 5.0 to 15.8 GeV/c. First, we describe the measurements with the newer experiment, E850, which had more complete kinematic definition of quasielastic events. E850 covered a larger range of incident momenta, and thus provided more information regarding the nature of the energy dependence of the nuclear transparency. In E850 the angular dependence of the nuclear transparency near 90 deg. and the nuclear transparency deuterons were studied. Second, we review the techniques used in an earlier experiment, E834, and show that the two experiments are consistent for the carbon data. E834 also determines the nuclear transparencies for lithium, aluminum, copper, and lead nuclei as well as for carbon. A determination of the (π + ,π + p) transparencies is also reported. We find for both E850 and E834 that the A(p,2p) nuclear transparency, unlike that for A(e,e ' p) nuclear transparency, is incompatible with a constant value versus energy as predicted by Glauber calculations. The A(p,2p) nuclear transparency for carbon and aluminum increases by a factor of two between 5.9 and 9.5 GeV/c incident proton momentum. At its peak the A(p,2p) nuclear transparency is ∼80% of the constant A(e,e ' p) nuclear transparency. Then the nuclear transparency falls back to a value at least as small as that at 5.9 GeV/c, and is compatible with the Glauber level again. This oscillating behavior is generally interpreted as an interplay between two components of the pN scattering amplitude; one short ranged and perturbative, and the other long ranged and strongly absorbed

  19. Human milk 90K (Mac-2 BP): possible protective effects against acute respiratory infections.

    Science.gov (United States)

    Fornarini, B; Iacobelli, S; Tinari, N; Natoli, C; De Martino, M; Sabatino, G

    1999-01-01

    Eighty-six children fed human milk were followed prospectively from birth to 12 months of age to assess the effect of milk 90K, a secreted glycoprotein with immune-stimulatory properties, on development of acute respiratory infections (ARI). The level of human milk 90K was inversely related to episodes of ARI (r = - 0.34; P = 0.001). The average 90K level in human milk fed to children who did not develop ARI was significantly higher than in milk fed to children in whom infection occurred on multiple occasions (156.6 +/- 144.8 microg/ml versus 70.9 +/- 92.3 microg/ml; P = 0.001). These data suggest that the protective effects of human milk against ARI may be due in part to immune maturation effects by secreted 90K.

  20. Study of Gamow-Teller giant resonance in /sup 90/Nb by the /sup 90/Zr(/sup 3/He,t)/sup 90/Nb reaction at 90 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Fujiwara, M.; Fujita, Y.; Katayama, I.; Morinobu, S.; Yamazaki, T.; Itahashi, T.; Ikegami, H. [Osaka Univ., Suita (Japan). Research Center for Nuclear Physics; Hayakawa, S. I.; Ikegami, Hidetsugu; Muraoka, Mitsuo [eds.; Osaka Univ., Suita (Japan). Research Center for Nuclear Physics

    1980-01-01

    A Gamow-Teller giant resonance in /sup 90/Nb was excited by the /sup 90/Zr(/sup 3/He, t) reaction at 89.5 MeV. The strength of the resonance was localized in the energy region of Ex = 4.5 - 7.5 MeV. The transition was found to be dominated by the L = 2 transfer.

  1. Treatment of persistent knee effusions with Yttrium 90

    International Nuclear Information System (INIS)

    Bouyoucef, S.E.; Drahmoune, R.; Mechken, F.; Amimour, A.; Hanni-Haddam, F.; Abtroun, F.; Sellah, M.; Mansouri, B.

    2002-01-01

    Yttrium 90 intra-articular injection is used in persisting active joint of the knee, where medication has failed to resolve chronic inflammation. The effective dose delivered to the synovia is linked to Y 90 activity and depends on the size of the joint space, the synovial structure and thickness and the inflammatory activity of the synovitis. The amount of the injected activity of Y90 was estimated according the volume effusion in 28 pathologic knee of 18 patients aged 18 years and more (mean age 46 years). All patients have persistent knee effusions and most of them have rheumatoid arthritis but others had ankylosing spondylitis, Behcet disease, psoriatic arthritis. According the radiological classification of Steinbrocker, 19 pathological knees were in stage 1, 5 in stage 2 and 4 in stage 3. The mean value of the monthly removed volume of the synovial liquid from the pathological knee was determined during the last month preceding the radiosynoviorthesis and four groups were identified: V0 no evidence of effusion liquid, 0 ml 100ml. The activity of Y 90 was estimated in order to obtain a total of 100 Gray in the envelope of 3 spherical phantoms with the same range of volume as defined above. The lowest activity of Y90, 111 MBq (3mCi) was determined for V0 according a mean value of standard sizes of knees. An activity of 18 MBq (0.5mCi) was added for each stage of 50 ml, so 129 MBq (3.5 mCi) for G1, 148 MBq (4 mCi) for G2 and 166 MBq (4.5 mCi) for G3. Efficacy of Y90 treatment was clinically assessed in all patients according to three parameters: pain, hydrarthrosis and range of joint movement at 1, 3, 6 and 12 months. The results were excellent in 13 knees and good in 9 and for most of them the efficacy of Y 90 was observed after 6 months. The results were less good in 3 knees but with an initial good evolution for all at 1 month. For 4 knees, the efficacy of Y 90 was bad. Although the small number of patients, these results show a high rate, 75%, of successful

  2. Comparação dos anticorpos anti-reticulina e antiendomísio classe IGA para diagnóstico e controle da dieta na doença celíaca Comparison of IgA class reticulin and endomysium antibodies for diagnosis and control of the diet in celiac disease

    Directory of Open Access Journals (Sweden)

    Lorete Maria da Silva KOTZE

    1999-12-01

    Full Text Available Sensibilidade ao glúten é um estado de elevada resposta iamunológica (celular e humoral à ingestão de proteínas do glúten do trigo, centeio, cevada e aveia, em indivíduos geneticamente predispostos. A doença celíaca é sua expressão mais freqüente, variando as formas de apresentação. Tem como tratamento a exclusão de alimentos contendo as gliadinas tóxicas. Embora a biopsia do intestino delgado proximal seja necessária, tem-se ressaltado a importância de testes sorológicos no rastreamento, diagnóstico e monitorização da dieta isenta de glúten em pacientes com doença celíaca. O objetivo do presente estudo foi investigar a presença dos anticorpos antiendomísio (EmA-IgA e anti-reticulina (ARA-IgA em 56 pacientes celíacos (17 recém diagnosticados; 24 aderentes à dieta; 15 com transgressão à dieta. Os anticorpos foram detectados por imunofluorescência indireta, utilizando como substrato cordão umbilical humano para os EmA-IgA, fígado e rim de rato para os ARA-IgA. Nos pacientes recém diagnosticados e no grupo com transgressão à dieta houve positividade total de 100% para os EmA-IgA e 59,4% para ARA-IgA. Nos pacientes aderentes à dieta nenhum dos anticorpos foi detectado. Dentre os 32 pacientes positivos, a concordância foi de 59,4% (19, sendo que 40,6 % (13/32 eram ARA-IgA negativo e EmA-IgA positivo. Nenhum paciente mostrou-se positivo para os ARA-IgA e negativo para os EmA-IgA. Portanto, a sensibilidade para os EmA-IgA foi de 100% e de 59,4% para os ARA-IgA. A associação dos dois testes não aumentou os índices de positividade total nas amostras. Conclui-se que, atualmente, a pesquisa dos EmA-IgA pode constituir teste sorológico de escolha, seja para diagnóstico, seja para seguimento dos pacientes celíacos, pelo alto valor preditivo, alta sensibilidade e especificidade e relativo baixo custo quando se utiliza cordão umbilical humano como substrato.Sensibility to gluten is a condition with high

  3. Neurological manifestations of celiac disease Manifestações neurológicas da doença celíaca

    Directory of Open Access Journals (Sweden)

    José Ibiapina Siqueira Neto

    2004-12-01

    Full Text Available Celiac disease (CD/ Nontropicalsprue, gluten-sensitive enteropathy is a malabsortive condition in which an allergic reaction to the cereal grain-protein gluten (present in wheat, rye and barley causes small intestine mucosal injury. The onset is in the first four decades of life, with a female to male ratio of 2:1. It may be associated with a wide spectrum of neurological manifestations including cerebellar ataxia, epileptic seizures, dementia, neuropathy, myopathy and multifocal leucoencephalopathy. We report three patients with neurological manifestations related with CD: one with cerebellar ataxia, one with epilepsy and one with cognitive impairment. The diagnosis of CD was confirmed by serologic tests (antiendomysial and antigliadin antibodies and biopsy of the small intestine. In two patients the neurological symptoms preceded the gastrointestinal abnormalities and in all of them gluten restriction failed to improve the neurological disability. Conclusion: CD should be ruled out in the differential diagnosis of neurological dysfunction of unknown cause, including ataxia, epilepsy and dementia. A gluten free diet, the mainstay of treatment, failed to improve the neurological disability.A doença celíaca (DC, enteropatia sensível ao glúten é desordem caracterizada por mal absorção causada por reação alérgica ao glúten, proteína presente em diversos cereais. As manifestações iniciais ocorrem nas primeiras quatro décadas de vida, sendo cerca de duas vezes mais freqüente no sexo feminino. DC pode estar associada a largo espectro de manifestações neurológicas incluindo ataxia cerebelar, epilepsia, demência, neuropatia, miopatia e leucoencefalopatia multifocal. Relatamos três casos de pacientes com manifestações neurológicas da DC: um com ataxia cerebelar, outro com epilepsia e o último com déficit cognitivo. O diagnóstico de DC foi estabelecido com base em testes sorológicos (anticorpos antiendomísio e antigliadina e

  4. Somatostatin-based radiotherapy with [90Y-DOTA]-TOC in neuroendocrine tumors: long-term outcome of a phase I dose escalation study

    Directory of Open Access Journals (Sweden)

    Marincek Nicolas

    2013-01-01

    Full Text Available Abstract Background We describe the long-term outcome after clinical introduction and dose escalation of somatostatin receptor targeted therapy with [90Y-DOTA]-TOC in patients with metastasized neuroendocrine tumors. Methods In a clinical phase I dose escalation study we treated patients with increasing [90Y-DOTA]-TOC activities. Multivariable Cox regression and competing risk regression were used to compare efficacy and toxicities of the different dosage protocols. Results Overall, 359 patients were recruited; 60 patients were enrolled for low dose (median: 2.4 GBq/cycle, range 0.9-7.8 GBq/cycle, 77 patients were enrolled for intermediate dose (median: 3.3 GBq/cycle, range: 2.0-7.4 GBq/cycle and 222 patients were enrolled for high dose (median: 6.7 GBq/cycle, range: 3.7-8.1 GBq/cycle [90Y-DOTA]-TOC treatment. The incidences of hematotoxicities grade 1–4 were 65.0%, 64.9% and 74.8%; the incidences of grade 4/5 kidney toxicities were 8.4%, 6.5% and 14.0%, and the median survival was 39 (range: 1–158 months, 34 (range: 1–118 months and 29 (range: 1–113 months. The high dose protocol was associated with an increased risk of kidney toxicity (Hazard Ratio: 3.12 (1.13-8.59 vs. intermediate dose, p = 0.03 and a shorter overall survival (Hazard Ratio: 2.50 (1.08-5.79 vs. low dose, p = 0.03. Conclusions Increasing [90Y-DOTA]-TOC activities may be associated with increasing hematological toxicities. The dose related hematotoxicity profile of [90Y-DOTA]-TOC could facilitate tailoring [90Y-DOTA]-TOC in patients with preexisting hematotoxicities. The results of the long-term outcome suggest that fractionated [90Y-DOTA]-TOC treatment might allow to reduce renal toxicity and to improve overall survival. (ClinicalTrials.gov number NCT00978211.

  5. Somatostatin-based radiotherapy with [90Y-DOTA]-TOC in neuroendocrine tumors: long-term outcome of a phase I dose escalation study

    Science.gov (United States)

    2013-01-01

    Background We describe the long-term outcome after clinical introduction and dose escalation of somatostatin receptor targeted therapy with [90Y-DOTA]-TOC in patients with metastasized neuroendocrine tumors. Methods In a clinical phase I dose escalation study we treated patients with increasing [90Y-DOTA]-TOC activities. Multivariable Cox regression and competing risk regression were used to compare efficacy and toxicities of the different dosage protocols. Results Overall, 359 patients were recruited; 60 patients were enrolled for low dose (median: 2.4 GBq/cycle, range 0.9-7.8 GBq/cycle), 77 patients were enrolled for intermediate dose (median: 3.3 GBq/cycle, range: 2.0-7.4 GBq/cycle) and 222 patients were enrolled for high dose (median: 6.7 GBq/cycle, range: 3.7-8.1 GBq/cycle) [90Y-DOTA]-TOC treatment. The incidences of hematotoxicities grade 1–4 were 65.0%, 64.9% and 74.8%; the incidences of grade 4/5 kidney toxicities were 8.4%, 6.5% and 14.0%, and the median survival was 39 (range: 1–158) months, 34 (range: 1–118) months and 29 (range: 1–113) months. The high dose protocol was associated with an increased risk of kidney toxicity (Hazard Ratio: 3.12 (1.13-8.59) vs. intermediate dose, p = 0.03) and a shorter overall survival (Hazard Ratio: 2.50 (1.08-5.79) vs. low dose, p = 0.03). Conclusions Increasing [90Y-DOTA]-TOC activities may be associated with increasing hematological toxicities. The dose related hematotoxicity profile of [90Y-DOTA]-TOC could facilitate tailoring [90Y-DOTA]-TOC in patients with preexisting hematotoxicities. The results of the long-term outcome suggest that fractionated [90Y-DOTA]-TOC treatment might allow to reduce renal toxicity and to improve overall survival. Trial registration ClinicalTrials.gov number:NCT00978211 PMID:23320604

  6. Relapse May Serve as a Mediator Variable in Longitudinal Outcomes in Multiple Sclerosis.

    Science.gov (United States)

    Stone, Lael Anne; Cutter, Gary Raymond; Fisher, Elizabeth; Richert, Nancy; McCartin, Jennifer; Ohayon, Joan; Bash, Craig; McFarland, Henry

    2016-05-01

    Contrast-enhancing lesions (CEL) on magnetic resonance imaging (MRI) are believed to represent inflammatory disease activity in multiple sclerosis (MS), but their relationship to subsequent long-term disability and progression is unclear, particularly at longer time periods such as 8-10 years. Between 1989 and 1994, 111 MS patients were seen at the National Institutes of Health for clinical evaluations and 3 monthly contrast-enhanced MRI scans. Of these, 94 patients were re-evaluated a mean of 8 years later (range 6.1-10.5 years) with a single MRI scan and clinical evaluation. CEL number and volume were determined at baseline and follow-up. The number of relapses was ascertained over the follow-up period and annualized relapse rates were calculated. Other MRI parameters, such as T2 hyperintensity volume, T1 volume, and brain parenchymal fraction, were also calculated. While there was no direct correlation between CEL number or volume at baseline and disability status at follow-up, CEL measures at baseline did correlate with number of relapses observed in the subsequent years, and the number of relapses in turn correlated with subsequent disability as well as transition to progressive MS. While number and volume of CEL at baseline do not directly correlate with disability in the longer term in MS, our data suggest that 1 route to disability involves relapses as a mediator variable in the causal sequence of MS progression from CEL to disability. Further studies using relapse as a mediator variable in a larger data set may be warranted. Copyright © 2015 by the American Society of Neuroimaging.

  7. Dosimetric comparison of electron beam and 90Sr+90Y applicator for keloids treatment

    International Nuclear Information System (INIS)

    Coelho, Talita S.; Tada, Ariane; Antonio, Patricia L.; Yoriyaz, Helio; Fernandes, Marco A.R.

    2009-01-01

    Studies have been shown that among several methods that have been used for the treatment of keloids the surgical excision followed by the adjuvant radiotherapy presents the lowest relapsed rate of the injury. In this work a comparative dosimetric study has been performed using a 4 MeV electron beam from a Varian Clinac 2100C linear accelerator at the radiotherapy service of the Hospital das Clinicas of UNESP-HC, Botucatu-SP and an Amershan 90 Sr+ 90 Y brachytherapy applicator with 1491 MBq of activity. Percentage depth dose curves from ionization chamber measurements and through Monte Carlo simulation have been obtained and compared. Dose measurements have been obtained using parallel plates ionization chamber (Esradin A12) and extrapolation mini-chamber developed at IPEN. The dose calculations have been obtained using the well-known Monte Carlo radiation transport code MCNP-4C. Maximum dose differences obtained between measured/calculated values for 90 Sr+ 90 Y applicator and for the electron beam were, respectively: 7.8 % and 8.0%. The profiles of the depth and superficial tissue dose distribution produced by the electron beam revealed themselves flatter and more homogeneous than those produced by the 90 Sr+ 90 Y applicator, especially to wider fields, which cannot be obtained with beta therapy applicators because of their geometric limitations. In conclusion this present work has shown that 90 Sr+ 90 Y applicators could be efficient for small and very superficial lesions but in most cases electron beam sources are more adequate especially for large and deeper lesions. (author)

  8. Temporary balloon occlusion of the common hepatic artery for administration of yttrium-90 resin microspheres in a patient with patent hepatoenteric collaterals.

    Science.gov (United States)

    Mahvash, Armeen; Zaer, Navid; Shaw, Colette; Chasen, Beth; Avritscher, Rony; Murthy, Ravi

    2012-02-01

    The most common serious complication of yttrium-90 ((90)Y) therapy is gastrointestinal ulceration caused by extrahepatic microsphere dispersion. The authors describe the use of a balloon catheter for temporary occlusion of the common hepatic artery to reverse hepatoenteric flow for lobar administration of resin microspheres when coil embolization of a retroportal artery was impossible. At 9 months after treatment, the patient had no gastrointestinal side effects and showed a partial response. Copyright © 2012 SIR. Published by Elsevier Inc. All rights reserved.

  9. Migration of strontium-90 from a strontium-90 fluoride deep ocean source

    International Nuclear Information System (INIS)

    Yabusaki, S.

    1981-06-01

    A hypothetical rupture of a heat source capsule on the ocean floor is analyzed for strontium-90 migration and attenuation. The evolution of the three-dimensional contaminant plume is simulated by a modified version of the Okubo-Pritchard radially symmetrical, diffusion velocity dispersion model. Results from this study indicate that released solutes are confined vertically to a layer near the level of introduction. Along the plume centerline, however, water quality is affected for considerably distances downstream from the source, with the maximum effect occurring after one year

  10. Analysis of Prognostic Factors After Yttrium-90 Radioembolization of Advanced Hepatocellular Carcinoma

    International Nuclear Information System (INIS)

    Inarrairaegui, Mercedes; Martinez-Cuesta, Antonio; Rodriguez, Macarena; Bilbao, J. Ignacio

    2010-01-01

    Purpose: To analyze which patient-, tumor-, and treatment-related factors may influence outcome after 90 Y radioembolization ( 90 Y-RE) for hepatocellular carcinoma (HCC). Patients and Methods: Seventy-two consecutive patients with advanced HCC treated with 90 Y-RE were studied to detect which factors may have influenced response to treatment and survival. Results: Median overall survival was 13 months (95% confidence interval, 9.6-16.3 months). In univariate analysis, survival was significantly better in patients with one to five lesions (19 vs. 8 months, p = 0.001) and in patients with alpha-fetoprotein 52 UI/mL, and their survival in the multivariate analysis was significantly worse (hazard ratio, 4.7; 95% confidence interval, 13-1.73) (p = 0.002). Conclusions: Yttrium-90 radioembolization results in control of target lesions in the majority of patients with HCC but does not prevent the development of new lesions. Survival of patients treated with 90 Y-RE seems to depend largely on factors related to the aggressiveness of the disease (number of nodules, levels of alpha-fetoprotein, and presence of microscopic disease).

  11. 40 CFR 90.205 - Banking.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Banking. 90.205 Section 90.205... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Certification Averaging, Banking, and Trading Provisions § 90.205 Banking. (a)(1) Beginning August 1, 2007, a manufacturer of a Class I engine...

  12. Tšernobõl põhjustab 90 000 vähisurma / Arko Olesk

    Index Scriptorium Estoniae

    Olesk, Arko, 1981-

    2006-01-01

    Vt. Postimees : na russkom jazõke 20. apr., lk. 9. Tuumajaama õnnetus 20 aasta eest Ukrainas Tšernobõlis tapab vähktõvega Euroopas ligikaudu 90 000 inimest, arvestab keskkonnaorganisatsiooni Greenpeace värske uurimus. Lisa: Eri hinnangud

  13. A primate specific extra domain in the molecular chaperone Hsp90.

    Directory of Open Access Journals (Sweden)

    Vishwadeepak Tripathi

    Full Text Available Hsp90 (heat shock protein 90 is an essential molecular chaperone that mediates folding and quality control of client proteins. Many of them such as protein kinases, steroid receptors and transcription factors are involved in cellular signaling processes. Hsp90 undergoes an ATP hydrolysis dependent conformational cycle to assist folding of the client protein. The canonical Hsp90 shows a typical composition of three distinct domains and interacts with individual cochaperone partners such as Hop, Cdc37 and Aha1 (activator of Hsp90 ATPase that regulate the reaction cycle of the molecular chaperone. A bioinformatic survey identified an additional domain of 122 amino acids in front of the canonical Hsp90 sequence. This extra domain (E domain is specific to the Catarrhini or drooping nose monkeys, a subdivision of the higher primates that includes man, the great apes and the old world monkeys but is absent from all other species. Our biochemical analysis reveals that Hsp103 associates with cochaperone proteins such as Hop, Cdc37 and Aha1 similar to Hsp90. However, the extra domain reduces the ATP hydrolysis rate to about half when compared to Hsp90 thereby acting as a negative regulator of the molecular chaperonés intrinsic ATPase activity.

  14. 22 CFR 226.90 - Disputes.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Disputes. 226.90 Section 226.90 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADMINISTRATION OF ASSISTANCE AWARDS TO U.S. NON-GOVERNMENTAL ORGANIZATIONS Miscellaneous § 226.90 Disputes. (a) Any dispute under or relating to a grant or agreement shall...

  15. Crustal structure of the Western Carpathians and Pannonian Basin System: seismic models from CELEBRATION 2000 data and geological implication

    Science.gov (United States)

    Janik, Tomasz; Grad, Marek; Guterch, Aleksander; Vozár, Jozef; Bielik, Miroslav; Vozárova, Anna; Hegedżs, Endre; Attila Kovács, Csaba; Kovács, István.; Celebration 2000 Working Group

    2010-05-01

    During CELEBRATION 2000 experiment the area of the Western Carphathians and Pannonian Basin System on the territory of southeastern Poland, Slovak Republic and Hungary was investigated by dense system of the deep seismic sounding profiles. In this paper, we present results of modelling of refracted and reflected waves with use 2-D ray tracing technique for profiles CEL01, CEL04, CEL05, CEL06, CEL11, CEL12 and CEL28. All seven profiles were jointly interpreted with verification and control the models at crossing points. Obtained P-wave velocity models of the crust and uppermost mantle are very complex and show differentiation of the seismic structure, where the depth of the Moho discontinuity is changing from about 25 to about 45 km. In the southern part of the area the relatively thin Pannonian Basin System crust consists of 3-7 km thick sediments and two crustal layers with 5.9-6.3 km/s in the upper crust and 6.3-6.6 km/s in the lower crust. In the upper crust of ALCAPA beneath profile CEL05 a high velocity body of Vp≥ 6.4 km/s was detected in the uppermost 5 km, which corresponds to the Bükk Composite Terrane. The total thickness of the ALCAPA crust is 1-2 km bigger than in the Tisza-Dacia. In the northern part of the area we observe 10-20 km thick uppermost crust with low velocity (Vp≤6.0 km/s), connected with TESZ and Carpathian Foredeep. Together with ca. 6.2-6.5 km/s and 6.5-6.9 km/s crustal layers they have a total thickness of 30-45 km (north of the Pieniny Klippen Belt). A sub-Moho velocities have in average values of 7.8-8.0 km/s for the Pannonian basin System, while in the Western Carpathian, the Trans-European suture zone (TESZ) and the East European Craton (EEC) part they are slightly bigger, 8.0-8.1 km/s. Lower velocities beneath the microplates ALCAPA and Tisza-Dacia could be caused by the different mineralogical and petrological compositions and the significant higher surface heat flow and temperature within the upper mantle. Beneath some

  16. Strontium-90 measurements in Switzerland

    International Nuclear Information System (INIS)

    Friedli, C.; Geering, J.J.; Lerch, P.

    1988-01-01

    Strontium-90 contamination in Switzerland has been measured since the sixties. The determination of 90 Sr in human vertebrae, milk-teeth, milk and wine have shown a maximum activity between 1964 and 1966. Since 1976, the survey has been extended to the food chain. After the Chernobyl accident, the measurements indicated a significative 90 Sr contamination in the Tessin and the north-east of Switzerland. The partition of 90 Sr in wheat has been determined and transfer factors are given for the milk - cheese - whey chain

  17. Gclust Server: 85848 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 85848 CEL_C08H9.5_17535345 Cluster Sequences Related Sequences(263) 502 old-1: Overexpression...ences Related Sequences(263) Sequence length 502 Representative annotation old-1: Overexpression

  18. GenBank blastx search result: AK242273 [KOME

    Lifescience Database Archive (English)

    Full Text Available novel pseudogene, the Havcr2 gene for hepatitis A virus cellular receptor 2, the gene for a novel protein similar to hepatitis... A virus cellular receptor 1 Havcr1, two hepatitis A virus cel...lular receptor (Havcr) pseudogenes, wo novel genes, a ribosomal protein L9 (Rpl9) pseudogene, the gene for a novel hepatitis

  19. Verification of ONED90 code

    International Nuclear Information System (INIS)

    Chang, Jong Hwa; Lee, Ki Bog; Zee, Sung Kyun; Lee, Chang Ho

    1993-12-01

    ONED90 developed by KAERI is a 1-dimensional 2-group diffusion theory code. For nuclear design and reactor simulation, the usage of ONED90 encompasses core follow calculation, load follow calculation, plant power control simulation, xenon oscillation simulation and control rod maneuvering, etc. In order to verify the validity of ONED90 code, two well-known benchmark problems are solved by ONED90 shows very similar result to reference solution. (Author) 11 refs., 5 figs., 13 tabs

  20. Rapid determination of 90Sr impurities in freshly 'generator eluted'90Y for radiopharmaceutical preparation

    International Nuclear Information System (INIS)

    Bonardi, Mauro L.; Martano, Luigi; Groppi, Flavia; Chinol, Marco

    2009-01-01

    90 Y is one of the most useful radionuclides for radioimmunotherapeutic applications and has a half-life (t 1/2 =64.14 h) suitable for most therapeutic applications, beta particles of high energy and decays to a stable daughter. It is significant that 90 Y is available conveniently and inexpensively from a radionuclide 'generator' by decay of its parent, 90 Sr. Nevertheless, current and planned clinical applications with [ 90 Y] labelled compounds employ activity levels that cannot be readily obtained from an in-house generator, but from commercial sources. We have evaluated Eichrom's Sr-resin, either as an 'in-house' generator or as a fast QC method for analysis of 90 Y solutions. In particular, for the development as a generator, we investigated the percentage of the radio-Sr in the first 8 M HNO 3 eluate: in this fraction the concentration of 90 Sr must be smaller than 10 -5 % (recommendations of the International Commission on Radiological Protection). For evaluation as a rapid QC method, we analyzed the concentration of 90 Y in all the fractions containing 'only' radio-Sr: 90 Y should not be present in these eluates. After the collection of β - and γ spectra and analysis of them, we concluded that commercial Sr-resin minicolumn cannot give us the results expected; we developed an in-house system loaded with 4 mL of Sr-resin which gave better results as a generator and a rapid QC method.

  1. Evaluation of Effective Parameters on Labeling of Hydroxyapatite Compound with 90Y and Introducing the Best Method to Produce 90Y-HAp Radiopharmaceutical for Radio synovectomy

    International Nuclear Information System (INIS)

    Davarpanah, M. R.; Khoshhosn, H. A.; Attar Nosrati, S.; Harati, S. M.; Aghamiri, S. M.; Ghannadi Maragheh, M.

    2012-01-01

    Radio synovectomy is a local intra-articular injection of radionuclides in colloidal form for treatment of articular inflammatory in rheumatoid arthritis, hemophilia or orthopedic troubles. β-emitting radionuclides can be used for various joints based on radiation energy. 90 Y is a pure β-emitter with a half-life of 64.1 hours that is used for treatment of the knee joint. β-radiation of this radionuclide possesses maximum energy of 2.281 MeV (99.98%), mean pathway of 3.6 mm in the soft tissue and maximum 11 mm. In this project, hydroxyapatite (HAp) is applied as a colloid maker agent that interacts with 90 Y 3+ ions via ion-dipole bonds and produces 90 Y-HAp. The colloidal pharmaceutical is produced by adding an acidic solution of 90 YCl 3 to an HAp suspension in saline. Effective parameters within which the colloid is applied, such as the volume of diluent, HAp particle size and sonication effect were evaluated and tested. First, these determinative parameters were optimized in the simulated conditions and then examined in the active phase. Finally, the best procedure was determined for the production of the radiopharmaceutical. Radionuclide purity of the radiopharmaceutical according to the primary 90 YCl 3 solution was over 99.9%. Labeling yield and radiochemical purity were obtained over 99% using TLC method in saline solvent up to three days after production of radiopharmaceutical. Radiochemical purity of 90 Y-HAp colloid was also evaluated in human serum albumin solution for three days at room temperature. The amount of released activity was between 0.3 to 2%.

  2. 28 CFR 90.21 - Evaluation.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Evaluation. 90.21 Section 90.21 Judicial...¢ Officers ⢠Prosecutors) Violence Against Women Formula Grant Program § 90.21 Evaluation. (a) The National Institute of Justice will conduct an evaluation of these programs. A portion of the overall funds authorized...

  3. Determination of [sup 90]Sr in environmental samples with resonance ionization spectroscopy in collinear geometry

    Energy Technology Data Exchange (ETDEWEB)

    Zimmer, K. (Inst. fuer Physik, Univ. Mainz (Germany)); Stenner, J. (Inst. fuer Physik, Univ. Mainz (Germany)); Kluge, H.J. (Inst. fuer Physik, Univ. Mainz (Germany)); Lantzsch, J. (Inst. fuer Physik, Univ. Mainz (Germany)); Monz, L. (Inst. fuer Physik, Univ. Mainz (Germany)); Otten, E.W. (Inst. fuer Physik, Univ. Mainz (Germany)); Passler, G. (Inst. fuer Physik, Univ. Mainz (Germany)); Schwalbach, R. (Inst. fuer Physik, Univ. Mainz (Germany)); Schwarz, M. (Inst. fuer Physik, Univ. Mainz (Germany)); Stevens, H. (Inst. fuer Physik, Univ. Mainz (Germany)); Wendt, K. (Inst. fuer Physik, Univ. Mainz (Germany)); Herrmann, G. (Inst. fuer Kernchemie, Univ. Mainz (Germany)); Niess, S. (Inst. fuer Kernchemie, Univ. Mainz (Germany)); Trautmann, N. (Inst. fuer Kernchemie, Univ. Mainz (Germany)); Walter, K. (Inst. fuer Kernchemie, Univ. Mainz (Germany)); Bushaw, B.A. (Pacific Northwest Lab., Richland, WA (United States))

    1994-08-01

    A new, fast technique for trace analysis of the radioactive isotopes [sup 89]Sr and [sup 90]Sr in environmental samples has been developed. Conventional mass separation is combined with resonance ionization spectroscopy in collinear geometry, which provides high selectivity and sensitivity. In addition, a chemical separation procedure for sample preparation has been developed. The described technique was used to determine the [sup 90]Sr content in [approx] 870 m[sup 3] air samples collected near Munich during and shortly after the Chernobyl reactor accident in April 1986. The content of [sup 90]Sr was measured to be 1.4 mBq per m[sup 3], corresponding to 1.6 x 10[sup 9] atoms of [sup 90]Sr per sample. This value is in good agreement with the results of radiochemical measurements. (orig.)

  4. Basic chromosome numbers and polyploid levels in some South African and Australian grasses (Poaceae

    Directory of Open Access Journals (Sweden)

    J. J. Spies

    1991-10-01

    Full Text Available Chromosome numbers of 46 specimens of grasses, involving 24 taxa from South Africa and Australia, have been determined during the present study. For the first time chromosome numbers are given for Eragrostis sarmentosa (Thunb. Trin. (n = 20. Panicum aequinerve Nees (n = 18,  Digitaria argyrograpta (Nees Stapf (n = 9 and D. maitlandii Stapf & C.E. Hubb. (n = 9. Additional polyploid levels are described for Diplachne fusca (L. Beauv. ex Roem. & Schult. (n = 10 and Digitaria diagonalis (Nees Stapf var.  diagonalis (n = 9. B-chromosomes were observed in several different specimens. The presence of B-chromosomes often results in abnormal chromosomal behaviour during meiosis.

  5. 45 CFR 98.90 - Monitoring.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Monitoring. 98.90 Section 98.90 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT FUND Monitoring, Non-compliance and Complaints § 98.90 Monitoring. (a) The Secretary will monitor programs funded under...

  6. 28 CFR 90.65 - Evaluation.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Evaluation. 90.65 Section 90.65 Judicial... Cases § 90.65 Evaluation. (a) The National Institute of Justice will conduct evaluations and studies of... with such federally-sponsored research and evaluation studies of their projects. In addition, grant...

  7. Heat shock protein 90β: A novel mediator of vitamin D action

    International Nuclear Information System (INIS)

    Angelo, Giana; Lamon-Fava, Stefania; Sonna, Larry A.; Lindauer, Meghan L.; Wood, Richard J.

    2008-01-01

    We investigated the role of Heat shock protein 90 (Hsp90) in vitamin D action in Caco-2 cells using geldanamycin (GA) to block Hsp90 function and RNA interference to reduce Hsp90β expression. When cells were exposed to GA, vitamin D-mediated gene expression and transcriptional activity were inhibited by 69% and 54%, respectively. Gel shift analysis indicated that GA reduced vitamin D-mediated DNA binding activity of the vitamin D receptor (VDR). We tested the specific role of Hsp90β by knocking down its expression with stably expressed short hairpin RNA. Vitamin D-induced gene expression and transcriptional activity were reduced by 90% and 80%, respectively, in Hsp90β-deficient cells. Nuclear protein for VDR and RXRα, its heterodimer partner, were not reduced in Hsp90β-deficient cells. These findings indicate that Hsp90β is needed for optimal vitamin D responsiveness in the enterocyte and demonstrate a specific role for Hsp90β in VDR signaling

  8. Half-life evaluations for 3H, 90Sr, and 90Y

    International Nuclear Information System (INIS)

    MacMahon, Desmond

    2006-01-01

    A recent paper has reviewed methods for the evaluation of discrepant sets of data and demonstrated the results of applying these methods to the published half-life data of 90 Sr and 137 Cs [MacMahon, T.D., Pearce, A., Harris, P., 2004. Convergence of techniques for the evaluation of discrepant data. Appl. Radiat. Isot. 60, 275-281]. The half-life data for 3 H has been subject to a comprehensive review and critical evaluation by Lucas and Unterweger [2000. Comprehensive review and critical evaluation of the half-life of tritium. J. Res. Natl. Inst. Stand. Technol. 105, 541-549]. The current paper reports the results of applying the various evaluation procedures of MacMahon et al. Convergence of techniques for the evaluation of discrepant data. Appl. Radiat. Isot. 60, 275-281] to the data of Lucas and Unterweger [Comprehensive review and critical evaluation of the half-life of tritium. J. Res. Natl. Inst. Stand. Technol. 105, 541-549], resulting in a recommended half-life of 4497(4) days. MacMahon et al. [Convergence of techniques for the evaluation of discrepant data. Appl. Radiat. Isot. 60, 275-281] highlighted problems in the evaluation of the discrepant half-life data of 90 Sr, in particular the worrying upward trend in the data, where the weighted mean of all the measurements increases, on average, by 35 days each time a new measurement result is added. The current paper reports on further analyses of these data. New measurements of the half-life of 90 Y have been reported by Kossert and Schrader [2004. Standardization by liquid scintillation counting and half-life measurements of 90 Y. Appl. Radiat. Isot. 60, 741]. This has prompted a new evaluation of all available published 90 Y half-life data. The data are fairly consistent, and a value of 64.063(16) h is recommended

  9. Producing a compound Nucleus via Inelastic Scattering: The 90Zr(alpha,alpha')90Zr* Case

    Energy Technology Data Exchange (ETDEWEB)

    Escher, J E; Dietrich, F S

    2008-05-23

    In a Surrogate reaction a compound nucleus is produced via a direct reaction (pickup, stripping, or inelastic scattering). For a proper application of the Surrogate approach it is necessary to predict the resulting angular momentum and parity distribution in the compound nucleus. A model for determining these distributions is developed for the case of inelastic alpha scattering off a spherical nucleus. The focus is on obtaining a first, simple description of the direct-reaction process that produces the compound nucleus and on providing the basis for a more complete treatment of the problem. The approximations employed in the present description are discussed and the extensions required for a more rigorous treatment of the problem are outlined. To illustrate the formalism, an application to {sup 90}Zr({alpha},{alpha}{prime}){sup 90}Zr* is presented.

  10. 15 CFR 90.14 - Hearing.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Hearing. 90.14 Section 90.14 Commerce... OF COMMERCE PROCEDURE FOR CHALLENGING CERTAIN POPULATION AND INCOME ESTIMATES § 90.14 Hearing. (a) The hearing shall be conducted by the same hearing officer who collected the documentary evidence, if...

  11. Somatostatin-based radiopeptide therapy with [177Lu-DOTA]-TOC versus [90Y-DOTA]-TOC in neuroendocrine tumours

    International Nuclear Information System (INIS)

    Romer, A.; Seiler, D.; Brunner, P.; Ng, Q.K.T.; Mueller-Brand, J.; Marincek, N.; Walter, M.A.; Koller, M.T.; Maecke, H.R.; Rochlitz, C.; Briel, M.; Schindler, C.

    2014-01-01

    Somatostatin-based radiopeptide treatment is generally performed using the β-emitting radionuclides 90 Y or 177 Lu. The present study aimed at comparing benefits and harms of both therapeutic approaches. In a comparative cohort study, patients with advanced neuroendocrine tumours underwent repeated cycles of [ 90 Y-DOTA]-TOC or [ 177 Lu-DOTA]-TOC until progression of disease or permanent adverse events. Multivariable Cox regression and competing risks regression were employed to examine predictors of survival and adverse events for both treatment groups. Overall, 910 patients underwent 1,804 cycles of [ 90 Y-DOTA]-TOC and 141 patients underwent 259 cycles of [ 177 Lu-DOTA]-TOC. The median survival after [ 177 Lu-DOTA]-TOC and after [ 90 Y-DOTA]-TOC was comparable (45.5 months versus 35.9 months, hazard ratio 0.91, 95 % confidence interval 0.63-1.30, p = 0.49). Subgroup analyses revealed a significantly longer survival for [ 177 Lu-DOTA]-TOC over [ 90 Y-DOTA]-TOC in patients with low tumour uptake, solitary lesions and extra-hepatic lesions. The rate of severe transient haematotoxicities was lower after [ 177 Lu-DOTA]-TOC treatment (1.4 vs 10.1 %, p = 0.001), while the rate of severe permanent renal toxicities was similar in both treatment groups (9.2 vs 7.8 %, p = 0.32). The present results revealed no difference in median overall survival after [ 177 Lu-DOTA]-TOC and [ 90 Y-DOTA]-TOC. Furthermore, [ 177 Lu-DOTA]-TOC was less haematotoxic than [ 90 Y-DOTA]-TOC. (orig.)

  12. Somatostatin-based radiopeptide therapy with [177Lu-DOTA]-TOC versus [90Y-DOTA]-TOC in neuroendocrine tumours.

    Science.gov (United States)

    Romer, A; Seiler, D; Marincek, N; Brunner, P; Koller, M T; Ng, Q K T; Maecke, H R; Müller-Brand, J; Rochlitz, C; Briel, M; Schindler, C; Walter, M A

    2014-02-01

    Somatostatin-based radiopeptide treatment is generally performed using the β-emitting radionuclides (90)Y or (177)Lu. The present study aimed at comparing benefits and harms of both therapeutic approaches. In a comparative cohort study, patients with advanced neuroendocrine tumours underwent repeated cycles of [(90)Y-DOTA]-TOC or [(177)Lu-DOTA]-TOC until progression of disease or permanent adverse events. Multivariable Cox regression and competing risks regression were employed to examine predictors of survival and adverse events for both treatment groups. Overall, 910 patients underwent 1,804 cycles of [(90)Y-DOTA]-TOC and 141 patients underwent 259 cycles of [(177)Lu-DOTA]-TOC. The median survival after [(177)Lu-DOTA]-TOC and after [(90)Y-DOTA]-TOC was comparable (45.5 months versus 35.9 months, hazard ratio 0.91, 95% confidence interval 0.63-1.30, p = 0.49). Subgroup analyses revealed a significantly longer survival for [(177)Lu-DOTA]-TOC over [(90)Y-DOTA]-TOC in patients with low tumour uptake, solitary lesions and extra-hepatic lesions. The rate of severe transient haematotoxicities was lower after [(177)Lu-DOTA]-TOC treatment (1.4 vs 10.1%, p = 0.001), while the rate of severe permanent renal toxicities was similar in both treatment groups (9.2 vs 7.8%, p = 0.32). The present results revealed no difference in median overall survival after [(177)Lu-DOTA]-TOC and [(90)Y-DOTA]-TOC. Furthermore, [(177)Lu-DOTA]-TOC was less haematotoxic than [(90)Y-DOTA]-TOC.

  13. Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris

    Directory of Open Access Journals (Sweden)

    Zhengyi Liu

    2014-01-01

    Full Text Available Heat shock protein 90 (HSP90, a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90 by combining homology cloning with rapid amplification of cDNA ends (RACE. Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5–45°C for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10°C, went further from 35 to 40°C, and reached the maximum at 40°C. On the other hand, for C. vulgaris kept at 35°C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (‰ was almost fourfold of that at 25 in salinity (‰ for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes.

  14. Safety evaluation of genetically modified DAS-40278-9 maize in a subchronic rodent feeding study.

    Science.gov (United States)

    Zou, Shiying; Lang, Tianqi; Liu, Xu; Huang, Kunlun; He, Xiaoyun

    2018-07-01

    Genetically modified (GM) maize, DAS-40278-9, expresses the aryloxyalkanoate dioxygenase-1 (AAD-1) protein, which confers tolerance to 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) herbicides. The aad-1 gene, which expresses the AAD-1 protein, was derived from Gram-negative soil bacterium, Sphingobium herbicidovorans. A 90-day sub-chronic toxicity study was conducted on rats as a component of the safety evaluation of DAS-40278-9 maize. Rats were given formulated diets containing maize grain from DAS-40278-9 or a non-GM near isogenic control comparator at an incorporation rate of 12.5%, 25%, or 50% (w/w), respectively for 90 days. In addition, another group of rats was fed a basic rodent diet. Animals were evaluated by cage-side and hand-held detailed clinical observations, ophthalmic examinations, body weights/body weight gains, feed consumption, hematology, serum chemistry, selected organ weights, and gross and histopathological examinations. Under the condition of this study, DAS-40278-9 maize did not cause any treatment-related effects in rats compared with rats fed diets containing non-GM maize. Copyright © 2018 Elsevier Inc. All rights reserved.

  15. Study of radionuclide 90Sr-90Y on cell proliferation and apoptosis in benign prostatic hyperplasia

    International Nuclear Information System (INIS)

    Zhang Tong; Wei Wei; Zou Benjie; Liu Fang; Xu Zhishun

    2003-01-01

    Objective: To investigate the effect of 90 Sr- 90 Yon cell proliferation and apoptosis in benign prostatic hyperplasia. Methods: The apoptosis and expression of Ki-67 in benign prostatic hyperplasia (BPH) before and after irradiation 90 Sr- 90 Y were detected by transferase-mediated dUTP-biotin nick end labeling (TUNEL) method and immunohistochemical technique, respectively. Results: The proliferation index (PI) of BPH after 90 Sr- 90 Y irradiation was much lower than that before irradiation, but there was no significant change in apoptosis index (AI). Conclusion: Irradiation with 90 Sr- 90 Y could restrain cell proliferation of BPH, but could not induce apoptosis

  16. Gender Differences in Isokinetic Strength after 60 and 90 d Bed Rest

    Science.gov (United States)

    English, K. L.; Ploutz-Snyder, R. J.; Cromwell, R. L.; Ploutz-Snyder, L. L.

    2010-01-01

    Recent reports suggest that changes in muscle strength following disuse may differ between males and females. PURPOSE: To examine potential gender differences in strength changes following 60 and 90 d of experimental bed rest. METHODS: Isokinetic extensor and flexor strength of the knee (60deg and 180deg/s, concentric only), ankle (30deg/s, concentric and eccentric), and trunk (60deg/s, concentric only) were measured following 60 d (males: n=4, 34.5+/-9.6 y; females: n=4, 35.5+/-8.2 y) and 90 d (males: n=10, 31.4+/-4.8 y; females: n=5, 37.6+/-9.9 y) of 6-degree head-down-tilt bed rest (BR; N=23). Subjects were fed a controlled diet (55%/15%/ 30%, CHO/PRO/FAT) that maintained body weight within 3% of the weight recorded on Day 3 of bed rest. After a familiarization session, testing was conducted 6 d before BR and 2 d after BR completion. Peak torque and total work were calculated for the tests performed. To allow us to combine data from both 60- and 90-d subjects, we used a mixed-model statistical analysis in which time and gender were fixed effects and bed rest duration was a random effect. Log-transformations of strength measures were utilized when necessary in order to meet statistical assumptions. RESULTS: Main effects were seen for both time and gender (p<0.05), showing decreased strength in response to bed rest for both males and females, and males stronger than females for most strength measures. Only one interaction effect was observed: females exhibited a greater loss of trunk extensor peak torque at 60 d versus pre-BR, relative to males (p=0.004). CONCLUSION: Sixty and 90 d of BR induced significant losses in isokinetic muscle strength of the locomotor and postural muscles of the knee, ankle, and trunk. Although males were stronger than females for most of the strength measures that we examined, only changes in trunk extensor peak torque were greater for females than males at day 60 of bed rest

  17. Gene ontology based transfer learning for protein subcellular localization

    Directory of Open Access Journals (Sweden)

    Zhou Shuigeng

    2011-02-01

    protein subcellular localization. We evaluate GO-TLM performance against three baseline models: MultiLoc, MultiLoc-GO and Euk-mPLoc on the benchmark datasets the baseline models adopted. 5-fold cross validation experiments show that GO-TLM achieves substantial accuracy improvement against the baseline models: 80.38% against model Euk-mPLoc 67.40% with 12.98% substantial increase; 96.65% and 96.27% against model MultiLoc-GO 89.60% and 89.60%, with 7.05% and 6.67% accuracy increase on dataset MultiLoc plant and dataset MultiLoc animal, respectively; 97.14%, 95.90% and 96.85% against model MultiLoc-GO 83.70%, 90.10% and 85.70%, with accuracy increase 13.44%, 5.8% and 11.15% on dataset BaCelLoc plant, dataset BaCelLoc fungi and dataset BaCelLoc animal respectively. For BaCelLoc independent sets, GO-TLM achieves 81.25%, 80.45% and 79.46% on dataset BaCelLoc plant holdout, dataset BaCelLoc plant holdout and dataset BaCelLoc animal holdout, respectively, as compared against baseline model MultiLoc-GO 76%, 60.00% and 73.00%, with accuracy increase 5.25%, 20.45% and 6.46%, respectively. Conclusions Since direct homology-based GO term transfer may be prone to introducing noise and outliers to the target protein, we design an explicitly weighted kernel learning system (called Gene Ontology Based Transfer Learning Model, GO-TLM to transfer to the target protein the known knowledge about related homologous proteins, which can reduce the risk of outliers and share knowledge between homologous proteins, and thus achieve better predictive performance for protein subcellular localization. Cross validation and independent test experimental results show that the homology-based GO term transfer and explicitly weighing the GO kernels substantially improve the prediction performance.