The Facial Expression Action Stimulus Test. A test battery for the assessment of face memory, face and object perception, configuration processing and facial expression recognition

Directory of Open Access Journals (Sweden)

Beatrice eDe Gelder

2015-10-01

Full Text Available There are many ways to assess face perception skills. In this study, we describe a novel task battery FEAST (Facial Expression Action Stimulus Test developed to test recognition of identity and expressions of human faces as well as stimulus control categories. The FEAST consists of a neutral and emotional face memory task, a face and object identity matching task, a face and house part-to-whole matching task, and a human and animal facial expression matching task. The identity and part-to-whole matching tasks contain both upright and inverted conditions. The results provide reference data of a healthy sample of controls in two age groups for future users of the FEAST.

Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.

Science.gov (United States)

Ruppert, Martin; Woll, Jörn; Giritch, Anatoli; Genady, Ezzat; Ma, Xueyan; Stöckigt, Joachim

2005-11-01

Acetylajmalan esterase (AAE) plays an essential role in the late stage of ajmaline biosynthesis. Based on the partial peptide sequences of AAE isolated and purified from Rauvolfia cell suspensions, a full-length AAE cDNA clone was isolated. The amino acid sequence of AAE has the highest level of identity of 40% to putative lipases known from the Arabidopsis thaliana genome project. Based on the primary structure AAE is a new member of the GDSL lipase superfamily. The expression in Escherichia coli failed although a wide range of conditions were tested. With a novel virus-based plant expression system, it was possible to express AAE functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity was detected in the Nicotiana tissue, which exceeded that in Rauvolfia serpentina (L.) Benth. ex Kurz cell suspension cultures about 20-fold. This expression allowed molecular analysis of AAE for the first time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six.

The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems.

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Tamer Z Salem

Full Text Available The simian virus 40 polyadenylation signal (SV40 polyA has been routinely inserted downstream of the polyhedrin promoter in many baculovirus expression vector systems (BEVS. In the baculovirus prototype Autographa californica multiple nucleopolyhedrovirus (AcMNPV, the polyhedrin promoter (very late promoter transcribes its gene by a viral RNA polymerase therefore there is no supporting evidence that SV40 polyA is required for the proper gene expression under the polyhedrin promoter. Moreover, the effect of the SV40 polyA sequence on the polyhedrin promoter activity has not been tested either at its natural polyhedrin locus or in other loci in the viral genome. In order to test the significance of adding the SV40 polyA sequence on gene expression, the expression of the enhanced green fluorescent protein (egfp was evaluated with and without the presence of SV40 polyA under the control of the polyhedrin promoter at different genomic loci (polyherin, ecdysteroid UDP-glucosyltransferase (egt, and gp37. In this study, spectrofluorometry and western blot showed reduction of EGFP protein for all recombinant viruses with SV40 polyA, whereas qPCR showed an increase in the egfp mRNA levels. Therefore, we conclude that SV40 polyA increases mRNA levels but decreases protein production in the BEVS when the polyhedrin promoter is used at different loci. This work suggests that SV40 polyA in BEVSs should be replaced by an AcMNPV late gene polyA for optimal protein production or left untouched for optimal RNA production (RNA interference applications.

The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems

Science.gov (United States)

Salem, Tamer Z.; Seaborn, Craig P.; Turney, Colin M.; Xue, Jianli; Shang, Hui; Cheng, Xiao-Wen

2015-01-01

The simian virus 40 polyadenylation signal (SV40 polyA) has been routinely inserted downstream of the polyhedrin promoter in many baculovirus expression vector systems (BEVS). In the baculovirus prototype Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the polyhedrin promoter (very late promoter) transcribes its gene by a viral RNA polymerase therefore there is no supporting evidence that SV40 polyA is required for the proper gene expression under the polyhedrin promoter. Moreover, the effect of the SV40 polyA sequence on the polyhedrin promoter activity has not been tested either at its natural polyhedrin locus or in other loci in the viral genome. In order to test the significance of adding the SV40 polyA sequence on gene expression, the expression of the enhanced green fluorescent protein (egfp) was evaluated with and without the presence of SV40 polyA under the control of the polyhedrin promoter at different genomic loci (polyherin, ecdysteroid UDP-glucosyltransferase (egt), and gp37). In this study, spectrofluorometry and western blot showed reduction of EGFP protein for all recombinant viruses with SV40 polyA, whereas qPCR showed an increase in the egfp mRNA levels. Therefore, we conclude that SV40 polyA increases mRNA levels but decreases protein production in the BEVS when the polyhedrin promoter is used at different loci. This work suggests that SV40 polyA in BEVSs should be replaced by an AcMNPV late gene polyA for optimal protein production or left untouched for optimal RNA production (RNA interference applications). PMID:26659470

Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.

Science.gov (United States)

Heiss, Silvia; Hörmann, Angelika; Tauer, Christopher; Sonnleitner, Margot; Egger, Esther; Grabherr, Reingard; Heinl, Stefan

2016-03-10

Engineering lactic acid bacteria (LAB) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. Often, expression of a transgene is only desired at a certain time point or period, e.g. to minimize the metabolic burden for the host cell or to control the expression time span. For this purpose, inducible expression systems are preferred, though cost and availability of the inducing agent must be feasible. We selected the plasmid free strain Lactobacillus plantarum 3NSH for testing and characterization of novel inducible promoters/repressor systems. Their feasibility in recombinant protein production was evaluated. Expression of the reporter protein mCherry was monitored with the BioLector(®) micro-fermentation system. Reporter gene mCherry expression was compared under the control of different promoter/repressor systems: PlacA (an endogenous promoter/repressor system derived from L. plantarum 3NSH), PxylA (a promoter/repressor system derived from Bacillus megaterium DSMZ 319) and PlacSynth (synthetic promoter and codon-optimized repressor gene based on the Escherichia coli lac operon). We observed that PlacA was inducible solely by lactose, but not by non-metabolizable allolactose analoga. PxylA was inducible by xylose, yet showed basal expression under non-induced conditions. Growth on galactose (as compared to exponential growth phase on glucose) reduced basal mCherry expression at non-induced conditions. PlacSynth was inducible with TMG (methyl β-D-thiogalactopyranoside) and IPTG (isopropyl β-D-1-thiogalactopyranoside), but also showed basal expression without inducer. The promoter PlacSynth was used for establishment of a dual plasmid expression system, based on T7 RNA polymerase driven expression in L. plantarum. Comparative Western blot supported BioLector(®) micro-fermentation measurements. Conclusively, overall expression levels were moderate (compared to a

Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems

Energy Technology Data Exchange (ETDEWEB)

Conover, David R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Crawford, Aladsair J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Fuller, Jason C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Gourisetti, Sri Nikhil Gup [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Viswanathan, Vilayanur V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ferreira, Summer [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Schoenwald, David [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Rosewater, David [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

2016-04-01

The Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems (PNNL-22010) was first issued in November 2012 as a first step toward providing a foundational basis for developing an initial standard for the uniform measurement and expression of energy storage system (ESS) performance. Based on experiences with the application and use of that document, and to include additional ESS applications and associated duty cycles, test procedures and performance metrics, a first revision of the November 2012 Protocol was issued in June 2014 (PNNL 22010 Rev. 1). As an update of the 2014 revision 1 to the Protocol, this document (the March 2016 revision 2 to the Protocol) is intended to supersede the June 2014 revision 1 to the Protocol and provide a more user-friendly yet more robust and comprehensive basis for measuring and expressing ESS performance.

The change of expression configuration affects identity-dependent expression aftereffect but not identity-independent expression aftereffect

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Miao eSong

2015-12-01

Full Text Available The present study examined the influence of expression configuration on cross-identity expression aftereffect. The expression configuration refers to the spatial arrangement of facial features in a face for conveying an emotion, e.g., an open-mouth smile versus a closed-mouth smile. In the first of two experiments, the expression aftereffect is measured using across-identity/cross-expression configuration factorial design. The facial identities of test faces were the same or different from the adaptor, while orthogonally, the expression configurations of those facial identities were also the same or different. The result shows that the change of expression configuration impaired the expression aftereffect when the facial identities of adaptor and tests were the same; however, the impairment effect disappears when facial identities were different, indicating the identity-independent expression representation is more robust to the change of the expression configuration in comparison with the identity-dependent expression representation. In the second experiment, we used schematic line faces as adaptors and real faces as tests to minimize the similarity between the adaptor and tests, which is expected to exclude the contribution from the identity-dependent expression representation to expression aftereffect. The second experiment yields a similar result as the identity-independent expression aftereffect observed in Experiment 1. The findings indicate the different neural sensitivities to expression configuration for identity-dependent and identity-independent expression systems.

Expression in E. coli systems

DEFF Research Database (Denmark)

Krogsdam, Anne-M; Kristiansen, Karsten; Nøhr, Jane

2003-01-01

intracellularly in soluble form. In E. coli, proteins containing disulfide bonds are best produced by secretion because the disulfide forming foldases reside in the periplasm. Likewise, a correct N-terminus is more likely to be obtained upon secretion. Moreover, potentially toxic proteins are more likely......Owing to cost advantage, speed of production, and often high product yield (up to 50% of total cell protein), expression in Escherichia coli is generally the first choice when attempting to express a recombinant protein. Expression systems exist to produce recombinant protein intracellularly...

FPGA-accelerated algorithm for the regular expression matching system

Science.gov (United States)

Russek, P.; Wiatr, K.

2015-01-01

This article describes an algorithm to support a regular expressions matching system. The goal was to achieve an attractive performance system with low energy consumption. The basic idea of the algorithm comes from a concept of the Bloom filter. It starts from the extraction of static sub-strings for strings of regular expressions. The algorithm is devised to gain from its decomposition into parts which are intended to be executed by custom hardware and the central processing unit (CPU). The pipelined custom processor architecture is proposed and a software algorithm explained accordingly. The software part of the algorithm was coded in C and runs on a processor from the ARM family. The hardware architecture was described in VHDL and implemented in field programmable gate array (FPGA). The performance results and required resources of the above experiments are given. An example of target application for the presented solution is computer and network security systems. The idea was tested on nearly 100,000 body-based viruses from the ClamAV virus database. The solution is intended for the emerging technology of clusters of low-energy computing nodes.

Small test SDHW systems

DEFF Research Database (Denmark)

Vejen, Niels Kristian

1999-01-01

Three small test SDHW systems was tested in a laboratory test facility.The three SDHW systems where all based on the low flow principe and a mantle tank but the design of the systems where different.......Three small test SDHW systems was tested in a laboratory test facility.The three SDHW systems where all based on the low flow principe and a mantle tank but the design of the systems where different....

A new efficient statistical test for detecting variability in the gene expression data.

Science.gov (United States)

Mathur, Sunil; Dolo, Samuel

2008-08-01

DNA microarray technology allows researchers to monitor the expressions of thousands of genes under different conditions. The detection of differential gene expression under two different conditions is very important in microarray studies. Microarray experiments are multi-step procedures and each step is a potential source of variance. This makes the measurement of variability difficult because approach based on gene-by-gene estimation of variance will have few degrees of freedom. It is highly possible that the assumption of equal variance for all the expression levels may not hold. Also, the assumption of normality of gene expressions may not hold. Thus it is essential to have a statistical procedure which is not based on the normality assumption and also it can detect genes with differential variance efficiently. The detection of differential gene expression variance will allow us to identify experimental variables that affect different biological processes and accuracy of DNA microarray measurements.In this article, a new nonparametric test for scale is developed based on the arctangent of the ratio of two expression levels. Most of the tests available in literature require the assumption of normal distribution, which makes them inapplicable in many situations, and it is also hard to verify the suitability of the normal distribution assumption for the given data set. The proposed test does not require the assumption of the distribution for the underlying population and hence makes it more practical and widely applicable. The asymptotic relative efficiency is calculated under different distributions, which show that the proposed test is very powerful when the assumption of normality breaks down. Monte Carlo simulation studies are performed to compare the power of the proposed test with some of the existing procedures. It is found that the proposed test is more powerful than commonly used tests under almost all the distributions considered in the study. A

Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis.

Science.gov (United States)

Linares, Daniel M; Alvarez-Sieiro, Patricia; del Rio, Beatriz; Ladero, Victor; Redruello, Begoña; Martin, Ma Cruz; Fernandez, Maria; Alvarez, Miguel A

2015-12-30

Lactococcus lactis has been safely consumed in fermented foods for millennia. This Gram-positive bacterium has now become of industrial importance as an expression host for the overproduction of lipopolysaccharide-free recombinant proteins used as food ingredients, therapeutic proteins and biotechnological enzymes. This paper reports an agmatine-controlled expression (ACE) system for L. lactis, comprising the lactococcal agmatine-sensor/transcriptional activator AguR and its target promoter P(aguB). The usefulness and efficiency of this system was checked via the reporter gene gfp and by producing PEP (Myxococcus xanthus prolyl-endopeptidase), an enzyme of biomedical interest able to degrade the immunotoxic peptides produced during the gastrointestinal breakdown of gluten. The ACE system developed in this work was suitable for the efficient expression of the functional recombinant proteins GFP and PEP. The expression system was tightly regulated by the agmatine concentration and allowed high protein production without leakiness.

Expression of Hepatoma-derived growth factor family members in the adult central nervous system

Directory of Open Access Journals (Sweden)

Abouzied Mekky M

2006-01-01

Full Text Available Abstract Background Hepatoma-derived growth factor (HDGF belongs to a polypeptide family containing five additional members called HDGF related proteins 1–4 (HRP-1 to -4 and Lens epithelial derived growth factor. Whereas some family members such as HDGF and HRP-2 are expressed in a wide range of tissues, the expression of others is very restricted. HRP-1 and -4 are only expressed in testis, HRP-3 only in the nervous system. Here we investigated the expression of HDGF, HRP-2 and HRP-3 in the central nervous system of adult mice on the cellular level by immunohistochemistry. In addition we performed Western blot analysis of various brain regions as well as neuronal and glial cell cultures. Results HDGF was rather evenly expressed throughout all brain regions tested with the lowest expression in the substantia nigra. HRP-2 was strongly expressed in the thalamus, prefrontal and parietal cortex, neurohypophysis, and the cerebellum, HRP-3 in the bulbus olfactorius, piriform cortex and amygdala complex. HDGF and HRP-2 were found to be expressed by neurons, astrocytes and oligodendrocytes. In contrast, strong expression of HRP-3 in the adult nervous system is restricted to neurons, except for very weak expression in oligodendrocytes in the brain stem. Although the majority of neurons are HRP-3 positive, some like cerebellar granule cells are negative. Conclusion The coexpression of HDGF and HRP-2 in glia and neurons as well as the coexpression of all three proteins in many neurons suggests different functions of members of the HDGF protein family in cells of the central nervous system that might include proliferation as well as cell survival. In addition the restricted expression of HRP-3 point to a special function of this family member for neuronal cells.

Difficulties Using Standardized Tests to Identify the Receptive Expressive Gap in Bilingual Children's Vocabularies.

Science.gov (United States)

Gibson, Todd A; Oller, D Kimbrough; Jarmulowicz, Linda

2018-03-01

Receptive standardized vocabulary scores have been found to be much higher than expressive standardized vocabulary scores in children with Spanish as L1, learning L2 (English) in school (Gibson et al., 2012). Here we present evidence suggesting the receptive-expressive gap may be harder to evaluate than previously thought because widely-used standardized tests may not offer comparable normed scores. Furthermore monolingual Spanish-speaking children tested in Mexico and monolingual English-speaking children in the US showed other, yet different statistically significant discrepancies between receptive and expressive scores. Results suggest comparisons across widely used standardized tests in attempts to assess a receptive-expressive gap are precarious.

Safety testing of GM-rice expressing PHA-E lectin using a new animal test design

DEFF Research Database (Denmark)

Poulsen, Morten; Schrøder, Malene; Wilcks, Andrea

2007-01-01

The 90-day animal study is the core study for the safety assessment of genetically modified foods in the SAFOTEST project. The model compound tested in the 90-day study was a rice variety expressing the kidney bean Phaseolus vulgaris lectin agglutinin E-form (PHA-E lectin). Female Wistar rats were...... safety testing of genetically modified foods....

Production of lysosomal enzymes in plant-based expression systems

OpenAIRE

1996-01-01

The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which ...

Test System Impact on System Availability

DEFF Research Database (Denmark)

Pau, L. F.

1987-01-01

The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance of the sys......The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance...... of the system subject to monitoring and test. A time-dependent Markov model is presented, and applied in three cases, with examples of numerical results provided for preventive maintenance decisions, design of an automatic test system, buffer testing in computers, and data communications....

Expression of GAT1 in male reproductive system and its effects on reproduction in mice.

Science.gov (United States)

Zhang, JinFu; Gui, YaPing; Yuan, Tao; Bian, CuiDong; Guo, LiHe

2009-12-01

The present study was carried out to identify GABA (gamma-aminobutyric acid) transport protein I (GAT1) in male reproductive organs and to study the effect of GAT1 overexpression on the male reproductive system in GAT1 transgenic mice (TG). Expression and location of GAT1 in testes, epididymis, and sperm of wild-type (WT) mice were identified by immunohistochemistry and western-blot. Histological changes of testes, epididymis, and sperm of transgenic mice overexpressing GAT1 were detected by immunofluorenscent staining and haematoxylin and eosin (HE) staining. GAT1 expression was detected in the testes, epididymis, and sperm of non-transgenic mice. Vacuolization and deformity of spermatogenic cells were observed in the transgenic mice, but the epididymis was unremarkable. Immunofluorenscent staining showed that the number of diastrophic and decapitated sperm increased significantly in transgenic mice to 46.9% from 7.3% in nontransgenic mice. These results suggest that abnormal expression of GAT1 could result in spermiogenesis function injury, sperm paramorphia and dysgenesis.

Proportional and Integral Thermal Control System for Large Scale Heating Tests

Science.gov (United States)

Fleischer, Van Tran

2015-01-01

The National Aeronautics and Space Administration Armstrong Flight Research Center (Edwards, California) Flight Loads Laboratory is a unique national laboratory that supports thermal, mechanical, thermal/mechanical, and structural dynamics research and testing. A Proportional Integral thermal control system was designed and implemented to support thermal tests. A thermal control algorithm supporting a quartz lamp heater was developed based on the Proportional Integral control concept and a linearized heating process. The thermal control equations were derived and expressed in terms of power levels, integral gain, proportional gain, and differences between thermal setpoints and skin temperatures. Besides the derived equations, user's predefined thermal test information generated in the form of thermal maps was used to implement the thermal control system capabilities. Graphite heater closed-loop thermal control and graphite heater open-loop power level were added later to fulfill the demand for higher temperature tests. Verification and validation tests were performed to ensure that the thermal control system requirements were achieved. This thermal control system has successfully supported many milestone thermal and thermal/mechanical tests for almost a decade with temperatures ranging from 50 F to 3000 F and temperature rise rates from -10 F/s to 70 F/s for a variety of test articles having unique thermal profiles and test setups.

Expressing best practices in (risk) analysis and testing of safety-critical systems using patterns

DEFF Research Database (Denmark)

Herzner, Wolfgang; Sieverding, Sven; Kacimi, Omar

2014-01-01

The continuing pervasion of our society with safety-critical cyber-physical systems not only demands for adequate (risk) analysis, testing and verification techniques, it also generates growing experience on their use, which can be considered as important as the tools themselves for their efficient...

Testosterone regulates granzyme K expression in rat testes

Directory of Open Access Journals (Sweden)

Dutta Dibyendu

2017-10-01

Full Text Available Objective. Testosterone depletion induces increased germ cell apoptosis in testes. However, limited studies exist on genes that regulate the germ cell apoptosis. Granzymes (GZM are serine proteases that induce apoptosis in various tissues. Multiple granzymes, including GZMA, GZMB and GZMN, are present in testes. Th us, we investigated which granzyme may be testosterone responsive and possibly may have a role in germ cell apoptosis aft er testosterone depletion. Methods. Ethylene dimethane sulfonate (EDS, a toxicant that selectively ablates the Leydig cells, was injected into rats to withdraw the testosterone. The testosterone depletion effects after 7 days post-EDS were verified by replacing the testosterone exogenously into EDS-treated rats. Serum or testicular testosterone was measured by radioimmunoassay. Using qPCR, mRNAs of granzyme variants in testes were quantified. The germ cell apoptosis was identified by TUNEL assay and the localization of GZMK was by immunohistochemistry. Results. EDS treatment eliminated the Leydig cells and depleted serum and testicular testosterone. At 7 days post-EDS, testis weights were reduced 18% with increased germ cell apoptosis plus elevation GZMK expression. GZMK was not associated with TUNEL-positive cells, but was localized to stripped cytoplasm of spermatids. In addition, apoptotic round spermatids were observed in the caput epididymis. Conclusions. GZMK expression in testes is testosterone dependent. GZMK is located adjacent to germ cells in seminiferous tubules and the presence of apoptotic round spermatids in the epididymis suggest its role in the degradation of microtubules in ectoplasmic specializations. Thus, overexpression of GZMK may indirectly regulate germ cell apoptosis by premature release of round spermatids from seminiferous tubule lumen.

Test report - caustic addition system operability test procedure

International Nuclear Information System (INIS)

Parazin, R.E.

1995-01-01

This Operability Test Report documents the test results of test procedure WHC-SD-WM-OTP-167 ''Caustic Addition System Operability Test Procedure''. The Objective of the test was to verify the operability of the 241-AN-107 Caustic Addition System. The objective of the test was met

Multilevel Regulation of Bacterial Gene Expression with the Combined STAR and Antisense RNA System.

Science.gov (United States)

Lee, Young Je; Kim, Soo-Jung; Moon, Tae Seok

2018-03-16

Synthetic small RNA regulators have emerged as a versatile tool to predictably control bacterial gene expression. Owing to their simple design principles, small size, and highly orthogonal behavior, these engineered genetic parts have been incorporated into genetic circuits. However, efforts to achieve more sophisticated cellular functions using RNA regulators have been hindered by our limited ability to integrate different RNA regulators into complex circuits. Here, we present a combined RNA regulatory system in Escherichia coli that uses small transcription activating RNA (STAR) and antisense RNA (asRNA) to activate or deactivate target gene expression in a programmable manner. Specifically, we demonstrated that the activated target output by the STAR system can be deactivated by expressing two different types of asRNAs: one binds to and sequesters the STAR regulator, affecting the transcription process, while the other binds to the target mRNA, affecting the translation process. We improved deactivation efficiencies (up to 96%) by optimizing each type of asRNA and then integrating the two optimized asRNAs into a single circuit. Furthermore, we demonstrated that the combined STAR and asRNA system can control gene expression in a reversible way and can regulate expression of a gene in the genome. Lastly, we constructed and simultaneously tested two A AND NOT B logic gates in the same cell to show sophisticated multigene regulation by the combined system. Our approach establishes a methodology for integrating multiple RNA regulators to rationally control multiple genes.

Transgenic Arabidopsis Gene Expression System

Science.gov (United States)

Ferl, Robert; Paul, Anna-Lisa

2009-01-01

The Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors (an organism used to determine the quality of the surrounding environment) of their environment using real-time nondestructive Green Fluorescent Protein (GFP) imagery and traditional postflight analyses.

Modular and coordinated expression of immune system regulatory and signaling components in the developing and adult nervous system.

Science.gov (United States)

Monzón-Sandoval, Jimena; Castillo-Morales, Atahualpa; Crampton, Sean; McKelvey, Laura; Nolan, Aoife; O'Keeffe, Gerard; Gutierrez, Humberto

2015-01-01

During development, the nervous system (NS) is assembled and sculpted through a concerted series of neurodevelopmental events orchestrated by a complex genetic programme. While neural-specific gene expression plays a critical part in this process, in recent years, a number of immune-related signaling and regulatory components have also been shown to play key physiological roles in the developing and adult NS. While the involvement of individual immune-related signaling components in neural functions may reflect their ubiquitous character, it may also reflect a much wider, as yet undescribed, genetic network of immune-related molecules acting as an intrinsic component of the neural-specific regulatory machinery that ultimately shapes the NS. In order to gain insights into the scale and wider functional organization of immune-related genetic networks in the NS, we examined the large scale pattern of expression of these genes in the brain. Our results show a highly significant correlated expression and transcriptional clustering among immune-related genes in the developing and adult brain, and this correlation was the highest in the brain when compared to muscle, liver, kidney and endothelial cells. We experimentally tested the regulatory clustering of immune system (IS) genes by using microarray expression profiling in cultures of dissociated neurons stimulated with the pro-inflammatory cytokine TNF-alpha, and found a highly significant enrichment of immune system-related genes among the resulting differentially expressed genes. Our findings strongly suggest a coherent recruitment of entire immune-related genetic regulatory modules by the neural-specific genetic programme that shapes the NS.

SPECTR System Operational Test Report

International Nuclear Information System (INIS)

Landman, W.H. Jr.

2011-01-01

This report overviews installation of the Small Pressure Cycling Test Rig (SPECTR) and documents the system operational testing performed to demonstrate that it meets the requirements for operations. The system operational testing involved operation of the furnace system to the design conditions and demonstration of the test article gas supply system using a simulated test article. The furnace and test article systems were demonstrated to meet the design requirements for the Next Generation Nuclear Plant. Therefore, the system is deemed acceptable and is ready for actual test article testing.

Escherichia coli Protein Expression System for Acetylcholine Binding Proteins (AChBPs.

Directory of Open Access Journals (Sweden)

Nikita Abraham

Full Text Available Nicotinic acetylcholine receptors (nAChR are ligand gated ion channels, identified as therapeutic targets for a range of human diseases. Drug design for nAChR related disorders is increasingly using structure-based approaches. Many of these structural insights for therapeutic lead development have been obtained from co-crystal structures of nAChR agonists and antagonists with the acetylcholine binding protein (AChBP. AChBP is a water soluble, structural and functional homolog of the extracellular, ligand-binding domain of nAChRs. Currently, AChBPs are recombinantly expressed in eukaryotic expression systems for structural and biophysical studies. Here, we report the establishment of an Escherichia coli (E. coli expression system that significantly reduces the cost and time of production compared to the existing expression systems. E. coli can efficiently express unglycosylated AChBP for crystallography and makes the expression of isotopically labelled forms feasible for NMR. We used a pHUE vector containing an N-terminal His-tagged ubiquitin fusion protein to facilitate AChBP expression in the soluble fractions, and thus avoid the need to recover protein from inclusion bodies. The purified protein yield obtained from the E. coli expression system is comparable to that obtained from existing AChBP expression systems. E. coli expressed AChBP bound nAChR agonists and antagonists with affinities matching those previously reported. Thus, the E. coli expression system significantly simplifies the expression and purification of functional AChBP for structural and biophysical studies.

Interactive analysis of systems biology molecular expression data

Directory of Open Access Journals (Sweden)

Prabhakar Sunil

2008-02-01

Full Text Available Abstract Background Systems biology aims to understand biological systems on a comprehensive scale, such that the components that make up the whole are connected to one another and work through dependent interactions. Molecular correlations and comparative studies of molecular expression are crucial to establishing interdependent connections in systems biology. The existing software packages provide limited data mining capability. The user must first generate visualization data with a preferred data mining algorithm and then upload the resulting data into the visualization package for graphic visualization of molecular relations. Results Presented is a novel interactive visual data mining application, SysNet that provides an interactive environment for the analysis of high data volume molecular expression information of most any type from biological systems. It integrates interactive graphic visualization and statistical data mining into a single package. SysNet interactively presents intermolecular correlation information with circular and heatmap layouts. It is also applicable to comparative analysis of molecular expression data, such as time course data. Conclusion The SysNet program has been utilized to analyze elemental profile changes in response to an increasing concentration of iron (Fe in growth media (an ionomics dataset. This study case demonstrates that the SysNet software is an effective platform for interactive analysis of molecular expression information in systems biology.

Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system

Directory of Open Access Journals (Sweden)

Lalonde Sylvie

2003-03-01

Full Text Available Abstract Background The Arabidopsis genome contains nine sucrose transporter paralogs falling into three clades: SUT1-like, SUT2 and SUT4. The carriers differ in their kinetic properties. Many transport proteins are known to exist as oligomers. The yeast-based split ubiquitin system can be used to analyze the ability of membrane proteins to interact. Results Promoter-GUS fusions were used to analyze the cellular expression of the three transporter genes in transgenic Arabidopsis plants. All three fusion genes are co-expressed in companion cells. Protein-protein interactions between Arabidopsis sucrose transporters were tested using the split ubiquitin system. Three paralogous sucrose transporters are capable of interacting as either homo- or heteromers. The interactions are specific, since a potassium channel and a glucose transporter did not show interaction with sucrose transporters. Also the biosynthetic and metabolizing enzymes, sucrose phosphate phosphatase and sucrose synthase, which were found to be at least in part bound to the plasma membrane, did not specifically interact with sucrose transporters. Conclusions The split-ubiquitin system provides a powerful tool to detect potential interactions between plant membrane proteins by heterologous expression in yeast, and can be used to screen for interactions with membrane proteins as baits. Like other membrane proteins, the Arabidopsis sucrose transporters are able to form oligomers. The biochemical approaches are required to confirm the in planta interaction.

Error response test system and method using test mask variable

Science.gov (United States)

Gender, Thomas K. (Inventor)

2006-01-01

An error response test system and method with increased functionality and improved performance is provided. The error response test system provides the ability to inject errors into the application under test to test the error response of the application under test in an automated and efficient manner. The error response system injects errors into the application through a test mask variable. The test mask variable is added to the application under test. During normal operation, the test mask variable is set to allow the application under test to operate normally. During testing, the error response test system can change the test mask variable to introduce an error into the application under test. The error response system can then monitor the application under test to determine whether the application has the correct response to the error.

Comparison of small n statistical tests of differential expression applied to microarrays

Directory of Open Access Journals (Sweden)

Lee Anna Y

2009-02-01

Full Text Available Abstract Background DNA microarrays provide data for genome wide patterns of expression between observation classes. Microarray studies often have small samples sizes, however, due to cost constraints or specimen availability. This can lead to poor random error estimates and inaccurate statistical tests of differential expression. We compare the performance of the standard t-test, fold change, and four small n statistical test methods designed to circumvent these problems. We report results of various normalization methods for empirical microarray data and of various random error models for simulated data. Results Three Empirical Bayes methods (CyberT, BRB, and limma t-statistics were the most effective statistical tests across simulated and both 2-colour cDNA and Affymetrix experimental data. The CyberT regularized t-statistic in particular was able to maintain expected false positive rates with simulated data showing high variances at low gene intensities, although at the cost of low true positive rates. The Local Pooled Error (LPE test introduced a bias that lowered false positive rates below theoretically expected values and had lower power relative to the top performers. The standard two-sample t-test and fold change were also found to be sub-optimal for detecting differentially expressed genes. The generalized log transformation was shown to be beneficial in improving results with certain data sets, in particular high variance cDNA data. Conclusion Pre-processing of data influences performance and the proper combination of pre-processing and statistical testing is necessary for obtaining the best results. All three Empirical Bayes methods assessed in our study are good choices for statistical tests for small n microarray studies for both Affymetrix and cDNA data. Choice of method for a particular study will depend on software and normalization preferences.

Effects of prebiotics on immune system and cytokine expression.

Science.gov (United States)

Shokryazdan, Parisa; Faseleh Jahromi, Mohammad; Navidshad, Bahman; Liang, Juan Boo

2017-02-01

Nowadays, use of prebiotics as feed and food additives has received increasing interest because of the beneficial effects of prebiotics on the health of animals and humans. One of the beneficial effects of prebiotics is stimulation of immune system, which can be direct or indirect through increasing population of beneficial microbes or probiotics, especially lactic acid bacteria and bifidobacteria, in the gut. An important mechanism of action of probiotics and prebiotics, by which they can affect the immune system, is changing the expression of cytokines. The present review tried to summarize the findings of studies that investigated the effects of prebiotics on immune system with focusing on their effects on cytokine expression. Generally, most of reviewed studies indicated beneficial effects for prebiotics in terms of improving immune system, by increasing the expression of anti-inflammatory cytokines, while reducing the expressions of proinflammatory cytokines. However, most of studies mainly considered the indirect effects of prebiotics on the immune system (through changing the composition and population of gut microbiota), and their direct effects still need to be further studied using prebiotics with different degree of polymerization in different hosts.

Thrombomodulin Expression in Tissues From Dogs With Systemic Inflammatory Disease.

Science.gov (United States)

Kim, S D; Baker, P; DeLay, J; Wood, R D

2016-07-01

Thrombomodulin (TM) is a membrane glycoprotein expressed on endothelial cells, which plays a major role in the protein C anticoagulation pathway. In people with inflammation, TM expression can be down-regulated on endothelial cells and a soluble form released into circulation, resulting in increased risk of thrombosis and disseminated intravascular coagulation. TM is present in dogs; however, there has been minimal investigation of its expression in canine tissues, and the effects of inflammation on TM expression in canine tissues have not been investigated. The objective of this study was to evaluate endothelial TM expression in tissues from dogs with systemic inflammatory diseases. A retrospective evaluation of tissue samples of lung, spleen, and liver from dogs with and without systemic inflammatory diseases was performed using immunohistochemistry (IHC) and a modified manual IHC scoring system. TM expression was significantly reduced in all examined tissues in dogs diagnosed with septic peritonitis or acute pancreatitis. © The Author(s) 2016.

Development of a GAL4-VP16/UAS trans-activation system for tissue specific expression in Medicago truncatula.

Directory of Open Access Journals (Sweden)

Amélie Sevin-Pujol

Full Text Available Promoters with tissue-specific activity are very useful to address cell-autonomous and non cell autonomous functions of candidate genes. Although this strategy is widely used in Arabidopsis thaliana, its use to study tissue-specific regulation of root symbiotic interactions in legumes has only started recently. Moreover, using tissue specific promoter activity to drive a GAL4-VP16 chimeric transcription factor that can bind short upstream activation sequences (UAS is an efficient way to target and enhance the expression of any gene of interest. Here, we developed a collection of promoters with different root cell layers specific activities in Medicago truncatula and tested their abilities to drive the expression of a chimeric GAL4-VP16 transcription factor in a trans-activation UAS: β-Glucuronidase (GUS reporter gene system. By developing a binary vector devoted to modular Golden Gate cloning together with a collection of adapted tissue specific promoters and coding sequences we could test the activity of four of these promoters in trans-activation GAL4/UAS systems and compare them to "classical" promoter GUS fusions. Roots showing high levels of tissue specific expression of the GUS activity could be obtained with this trans-activation system. We therefore provide the legume community with new tools for efficient modular Golden Gate cloning, tissue specific expression and a trans-activation system. This study provides the ground work for future development of stable transgenic lines in Medicago truncatula.

Data Link Test and Analysis System/ATCRBS Transponder Test System Technical Reference

Science.gov (United States)

1990-05-01

This document references material for personnel using or making software changes : to the Data Link Test and Analysis System (DATAS) for Air Traffic Control Radar : Beacon System (ATCRBS) transponder testing and data collection. This is one of : a se...

Inspection system performance test procedure

International Nuclear Information System (INIS)

Jensen, C.E.

1995-01-01

This procedure establishes requirements to administer a performance demonstration test. The test is to demonstrate that the double-shell tank inspection system (DSTIS) supplied by the contractor performs in accordance with the WHC-S-4108, Double-Shell Tank Ultrasonic Inspection Performance Specification, Rev. 2-A, January, 1995. The inspection system is intended to provide ultrasonic (UT) and visual data to determine integrity of the Westinghouse Hanford Company (WHC) site underground waste tanks. The robotic inspection system consists of the following major sub-systems (modules) and components: Mobile control center; Deployment module; Cable management assembly; Robot mechanism; Ultrasonic testing system; Visual testing system; Pneumatic system; Electrical system; and Control system

Portable Health Algorithms Test System

Science.gov (United States)

Melcher, Kevin J.; Wong, Edmond; Fulton, Christopher E.; Sowers, Thomas S.; Maul, William A.

2010-01-01

A document discusses the Portable Health Algorithms Test (PHALT) System, which has been designed as a means for evolving the maturity and credibility of algorithms developed to assess the health of aerospace systems. Comprising an integrated hardware-software environment, the PHALT system allows systems health management algorithms to be developed in a graphical programming environment, to be tested and refined using system simulation or test data playback, and to be evaluated in a real-time hardware-in-the-loop mode with a live test article. The integrated hardware and software development environment provides a seamless transition from algorithm development to real-time implementation. The portability of the hardware makes it quick and easy to transport between test facilities. This hard ware/software architecture is flexible enough to support a variety of diagnostic applications and test hardware, and the GUI-based rapid prototyping capability is sufficient to support development execution, and testing of custom diagnostic algorithms. The PHALT operating system supports execution of diagnostic algorithms under real-time constraints. PHALT can perform real-time capture and playback of test rig data with the ability to augment/ modify the data stream (e.g. inject simulated faults). It performs algorithm testing using a variety of data input sources, including real-time data acquisition, test data playback, and system simulations, and also provides system feedback to evaluate closed-loop diagnostic response and mitigation control.

Hardware in the loop simulation test platform of fuel cell backup system

Directory of Open Access Journals (Sweden)

Ma Tiancai

2015-01-01

Full Text Available Based on an analysis of voltage mechanistic model, a real-time simulation model of the proton exchange membrane (PEM fuel cell backup system is developed, and verified by the measurable experiment data. The method of online parameters identification for the model is also improved. Based on the software LabVIEW/VeriStand real-time environment and the PXI Express hardware system, the PEM fuel cell system controller hardware in the loop (HIL simulation plat-form is established. Controller simulation test results showed the accuracy of HIL simulation platform.

Gene Expression Profiling of Peripheral Blood From Kidney Transplant Recipients for the Early Detection of Digestive System Cancer.

Science.gov (United States)

Kusaka, M; Okamoto, M; Takenaka, M; Sasaki, H; Fukami, N; Kataoka, K; Ito, T; Kenmochi, T; Hoshinaga, K; Shiroki, R

2017-06-01

Kidney transplant recipients are at increased risk of developing cancer in comparison with the general population. To effectively manage post-transplantation malignancies, it is essential to proactively monitor patients. A long-term intensive screening program was associated with a reduced incidence of cancer after transplantation. This study evaluated the usefulness of the gene expression profiling of peripheral blood samples obtained from kidney transplant patients and adopted a screening test for detecting cancer of the digestive system (gastric, colon, pancreas, and biliary tract). Nineteen patients were included in this study and a total of 53 gene expression screening tests were performed. The gene expression profiles of blood-delivered total RNA and whole genome human gene expression profiles were obtained. We investigated the expression levels of 2665 genes associated with digestive cancers and counted the number of genes in which expression was altered. A hierarchical clustering analysis was also performed. The final prediction of the cancer possibility was determined according to an algorithm. The number of genes in which expression was altered was significantly increased in the kidney transplant recipients in comparison with the general population (1091 ± 63 vs 823 ± 94; P = .0024). The number of genes with altered expression decreased after the induction of mechanistic target of rapamycin (mTOR) inhibitor (1484 ± 227 vs 883 ± 154; P = .0439). No cases of possible digestive cancer were detected in this study period. The gene expression profiling of peripheral blood samples may be a useful and noninvasive diagnostic tool that allows for the early detection of cancer of the digestive system. Copyright © 2017 Elsevier Inc. All rights reserved.

Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar

Directory of Open Access Journals (Sweden)

Houeix Benoit

2010-03-01

Full Text Available Abstract Background The male Atlantic salmon generally matures in fresh water upon returning after one or several years at sea. Some fast-growing male parr develop an alternative life strategy where they sexually mature before migrating to the oceans. These so called 'precocious' parr or 'sneakers' can successfully fertilise adult female eggs and so perpetuate their line. We have used a custom-built cDNA microarray to investigate gene expression changes occurring in the salmon gonad and brain associated with precocious maturation. The microarray has been populated with genes selected specifically for involvement in sexual maturation (precocious and adult and in the parr-smolt transformation. Results Immature and mature parr collected from a hatchery-reared stock in January were significantly different in weight, length and condition factor. Changes in brain expression were small - never more than 2-fold on the microarray, and down-regulation of genes was much more pronounced than up-regulation. Significantly changing genes included isotocin, vasotocin, cathepsin D, anamorsin and apolipoprotein E. Much greater changes in expression were seen in the testes. Among those genes in the testis with the most significant changes in expression were anti-Mullerian hormone, collagen 1A, and zinc finger protein (Zic1, which were down-regulated in precocity and apolipoproteins E and C-1, lipoprotein lipase and anti-leukoproteinase precursor which were up-regulated in precocity. Expression changes of several genes were confirmed in individual fish by quantitative PCR and several genes (anti-Mullerian hormone, collagen 1A, beta-globin and guanine nucleotide binding protein (G protein beta polypeptide 2-like 1 (GNB2L1 were also examined in adult maturing testes. Down-regulation of anti-Mullerian hormone was judged to be greater than 160-fold for precocious males and greater than 230-fold for November adult testes in comparison to July testes by this method. For

AVS/Express (application visualization system) user's guide

Energy Technology Data Exchange (ETDEWEB)

Kato, Katsumi [Research Organization for Information Science Technology, Tokai, Ibaraki (Japan); Kume, Etsuo [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

2002-09-01

Computer and network environment for image processing has been developed and maintained under the course of establishing a distributed processing environment by the information system operating division. We introduced a server for image processing, AVS/Express for image processing software and Stereo viewing system. This report summarizes the information to use AVS/Express efficiently in the computer environment for image processing. (author)

Connexin 43 expression in human and mouse testes with impaired spermatogenesis

Directory of Open Access Journals (Sweden)

M Kotula-Balak

2009-08-01

Full Text Available Connexin 43 (Cx43 belongs to a family of proteins that form gap junction channels. The aim of this study was to examine the expression of Cx43 in the testis of a patient with Klinefelter’s syndrome and of mice with the mosaic mutation and a partial deletion in the long arm of the Y chromosome. These genetic disorders are characterized by the presence of numerous degenerated seminiferous tubules and impaired spermatogenesis. In mouse testes, the expression and presence of Cx43 were detected by means of immunohistochemistry and Western blot analysis, respectively. In testes of Klinefelter’s patient only immunoexpression of Cx43 was detected. Regardless of the species Cx43 protein was ubiquitously distributed in testes of reproductively normal males, whereas in those with testicular disorders either a weak intensity of staining or no staining within the seminiferous tubules was observed. Moderate to strong or very strong staining was confined to the interstitial tissue. In an immunoblot analysis of testicular homogenates Cx43 appeared as one major band of approximately 43 kDa. Our study adds three more examples of pathological gonads in which the absence or apparent decrease of Cx43 expression within the seminiferous tubules was found. A positive correlation between severe spermatogenic impairment and loss of Cx43 immunoreactivity observed in this study supports previous data that gap junctions play a crucial role in spermatogenesis. Strong Cx43 expression detected mostly in the interstitial tissue of the Klinefelter’s patient may presumably be of importance in sustaining Leydig cell metabolic activity. However, the role of gap junction communication in the control of Leydig cell function seems to be more complex than originally thought.

Why do fearful facial expressions elicit behavioral approach? Evidence from a combined approach-avoidance implicit association test.

Science.gov (United States)

Hammer, Jennifer L; Marsh, Abigail A

2015-04-01

Despite communicating a "negative" emotion, fearful facial expressions predominantly elicit behavioral approach from perceivers. It has been hypothesized that this seemingly paradoxical effect may occur due to fearful expressions' resemblance to vulnerable, infantile faces. However, this hypothesis has not yet been tested. We used a combined approach-avoidance/implicit association test (IAT) to test this hypothesis. Participants completed an approach-avoidance lever task during which they responded to fearful and angry facial expressions as well as neutral infant and adult faces presented in an IAT format. Results demonstrated an implicit association between fearful facial expressions and infant faces and showed that both fearful expressions and infant faces primarily elicit behavioral approach. The dominance of approach responses to both fearful expressions and infant faces decreased as a function of psychopathic personality traits. Results suggest that the prosocial responses to fearful expressions observed in most individuals may stem from their associations with infantile faces. (PsycINFO Database Record (c) 2015 APA, all rights reserved).

A new maltose-inducible high-performance heterologous expression system in Bacillus subtilis.

Science.gov (United States)

Yue, Jie; Fu, Gang; Zhang, Dawei; Wen, Jianping

2017-08-01

To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the P malA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the P hpaII system. A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.

Gene expression-based biological test for major depressive disorder: an advanced study

Directory of Open Access Journals (Sweden)

Watanabe S

2017-02-01

Full Text Available Shin-ya Watanabe,1 Shusuke Numata,1 Jun-ichi Iga,2 Makoto Kinoshita,1 Hidehiro Umehara,1 Kazuo Ishii,3 Tetsuro Ohmori1 1Department of Psychiatry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 2Department of Neuropsychiatry, Molecules and Function, Ehime University Graduate School of Medicine, Ehime, 3Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan Purpose: Recently, we could distinguished patients with major depressive disorder (MDD from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4 in leukocyte. In the present study, we examined whether this biological test is able to discriminate patients with MDD from those without MDD, including those with schizophrenia and bipolar disorder.Patients and methods: We measured messenger ribonucleic acid expression levels of the aforementioned five genes in peripheral leukocytes in 17 patients with schizophrenia and 36 patients with bipolar disorder using quantitative real-time polymerase chain reaction (PCR, and we combined these expression data with our previous expression data of 25 patients with MDD and 25 controls. Subsequently, a linear discriminant function was developed for use in discriminating between patients with MDD and without MDD.Results: This expression panel was able to segregate patients with MDD from those without MDD with a sensitivity and specificity of 64% and 67.9%, respectively.Conclusion: Further research to identify MDD-specific markers is needed to improve the performance of this biological test. Keywords: depressive disorder, biomarker, gene expression, schizophrenia, bipolar disorder

Gene expression programming for power system static security ...

African Journals Online (AJOL)

user

Keywords: static security, gene expression programming, probabilistic neural network ... Hence digital computers are usually installed in operations control centers to gather ...... power system protection, and applications of AI in power systems.

Design of Tokamak synchronous data acquisition system based on PXI express

International Nuclear Information System (INIS)

Liu Rui; Zheng Wei; Zhang Ming; Weng Chuqiao; Zhuang Ge; Ding Tonghai; Yu Kexun

2014-01-01

With the development of J-TEXT device, the original data acquisition system can't meet the experiment's requirement on stability, modularity and sampling rate, so a new data acquisition system needs to be built. This paper introduces the design and implementation of the distributed Tokamak synchronous high-speed data acquisition system based on PXI Express. The acquisition unit consists of PXIe case Nl PXIe 1062Q, PXIe controller NI PXIe-8133 and high-speed synchronous data acquisition card Nl PXIe-6368, compatible with the latest standard of ITER CODAC, so it has good mechanical sealing, strong modularity and high sampling rate etc. The system takes a synchronous difference acquisition for diagnosis signal. The data storage adopts MDSplus which is the general database in the nuclear fusion field. The test and experimental results show that the system can work continuously and stably at 2 MSps sampling rate, and meet the requirement of experiment device's operation well. (authors)

Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor.

Science.gov (United States)

Kim, Seong Keun; Lee, Dae-Hee; Kim, Oh Cheol; Kim, Jihyun F; Yoon, Sung Ho

2017-09-15

Most inducible expression systems suffer from growth defects, leaky basal induction, and inhomogeneous expression levels within a host cell population. These difficulties are most prominent with the overproduction of membrane proteins that are toxic to host cells. Here, we developed an Escherichia coli inducible expression system for membrane protein production based on titrated expression of a mutant lac repressor (mLacI). Performance of the mLacI inducible system was evaluated in conjunction with commonly used lac operator-based expression vectors using a T7 or tac promoter. Remarkably, expression of a target gene can be titrated by the dose-dependent addition of l-rhamnose, and the expression levels were homogeneous in the cell population. The developed system was successfully applied to overexpress three membrane proteins that were otherwise difficult to produce in E. coli. This gene expression control system can be easily applied to a broad range of existing protein expression systems and should be useful in constructing genetic circuits that require precise output signals.

Gene expression-based molecular diagnostic system for malignant gliomas is superior to histological diagnosis.

Science.gov (United States)

Shirahata, Mitsuaki; Iwao-Koizumi, Kyoko; Saito, Sakae; Ueno, Noriko; Oda, Masashi; Hashimoto, Nobuo; Takahashi, Jun A; Kato, Kikuya

2007-12-15

Current morphology-based glioma classification methods do not adequately reflect the complex biology of gliomas, thus limiting their prognostic ability. In this study, we focused on anaplastic oligodendroglioma and glioblastoma, which typically follow distinct clinical courses. Our goal was to construct a clinically useful molecular diagnostic system based on gene expression profiling. The expression of 3,456 genes in 32 patients, 12 and 20 of whom had prognostically distinct anaplastic oligodendroglioma and glioblastoma, respectively, was measured by PCR array. Next to unsupervised methods, we did supervised analysis using a weighted voting algorithm to construct a diagnostic system discriminating anaplastic oligodendroglioma from glioblastoma. The diagnostic accuracy of this system was evaluated by leave-one-out cross-validation. The clinical utility was tested on a microarray-based data set of 50 malignant gliomas from a previous study. Unsupervised analysis showed divergent global gene expression patterns between the two tumor classes. A supervised binary classification model showed 100% (95% confidence interval, 89.4-100%) diagnostic accuracy by leave-one-out cross-validation using 168 diagnostic genes. Applied to a gene expression data set from a previous study, our model correlated better with outcome than histologic diagnosis, and also displayed 96.6% (28 of 29) consistency with the molecular classification scheme used for these histologically controversial gliomas in the original article. Furthermore, we observed that histologically diagnosed glioblastoma samples that shared anaplastic oligodendroglioma molecular characteristics tended to be associated with longer survival. Our molecular diagnostic system showed reproducible clinical utility and prognostic ability superior to traditional histopathologic diagnosis for malignant glioma.

A real-time control system of gene expression using ligand-bound nucleic acid aptamer for metabolic engineering.

Science.gov (United States)

Wang, Jing; Cui, Xun; Yang, Le; Zhang, Zhe; Lv, Liping; Wang, Haoyuan; Zhao, Zhenmin; Guan, Ningzi; Dong, Lichun; Chen, Rachel

2017-07-01

Artificial control of bio-functions through regulating gene expression is one of the most important and attractive technologies to build novel living systems that are useful in the areas of chemical synthesis, nanotechnology, pharmacology, cell biology. Here, we present a novel real-time control system of gene regulation that includes an enhancement element by introducing duplex DNA aptamers upstream promoter and a repression element by introducing a RNA aptamer upstream ribosome binding site. With the presence of ligands corresponding to the DNA aptamers, the expression of the target gene can be potentially enhanced at the transcriptional level by strengthening the recognition capability of RNAP to the recognition region and speeding up the separation efficiency of the unwinding region due to the induced DNA bubble around the thrombin-bound aptamers; while with the presence of RNA aptamer ligand, the gene expression can be repressed at the translational level by weakening the recognition capability of ribosome to RBS due to the shielding of RBS by the formed aptamer-ligand complex upstream RBS. The effectiveness and potential utility of the developed gene regulation system were demonstrated by regulating the expression of ecaA gene in the cell-free systems. The realistic metabolic engineering application of the system has also tested by regulating the expression of mgtC gene and thrombin cDNA in Escherichia coli JD1021 for controlling metabolic flux and improving thrombin production, verifying that the real-time control system of gene regulation is able to realize the dynamic regulation of gene expression with potential applications in bacterial physiology studies and metabolic engineering. Copyright © 2017. Published by Elsevier Inc.

Energy Systems Test Area (ESTA) Electrical Power Systems Test Operations: User Test Planning Guide

Science.gov (United States)

Salinas, Michael J.

2012-01-01

Test process, milestones and inputs are unknowns to first-time users of the ESTA Electrical Power Systems Test Laboratory. The User Test Planning Guide aids in establishing expectations for both NASA and non-NASA facility customers. The potential audience for this guide includes both internal and commercial spaceflight hardware/software developers. It is intended to assist their test engineering personnel in test planning and execution. Material covered includes a roadmap of the test process, roles and responsibilities of facility and user, major milestones, facility capabilities, and inputs required by the facility. Samples of deliverables, test article interfaces, and inputs necessary to define test scope, cost, and schedule are included as an appendix to the guide.

Systems reconsolidation reveals a selective role for the anterior cingulate cortex in generalized contextual fear memory expression.

Science.gov (United States)

Einarsson, Einar Ö; Pors, Jennifer; Nader, Karim

2015-01-01

After acquisition, hippocampus-dependent memories undergo a systems consolidation process, during which they become independent of the hippocampus and dependent on the anterior cingulate cortex (ACC) for memory expression. However, consolidated remote memories can become transiently hippocampus-dependent again following memory reactivation. How this systems reconsolidation affects the role of the ACC in remote memory expression is not known. Using contextual fear conditioning, we show that the expression of 30-day-old remote memory can transiently be supported by either the ACC or the dorsal hippocampus following memory reactivation, and that the ACC specifically mediates expression of remote generalized contextual fear memory. We found that suppression of neural activity in the ACC with the AMPA/kainate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) impaired the expression of remote, but not recent, contextual fear memory. Fear expression was not affected by this treatment if preceded by memory reactivation 6 h earlier, nor was it affected by suppression of neural activity in the dorsal hippocampus with the GABA-receptor agonist muscimol. However, simultaneous targeting of both the ACC and the dorsal hippocampus 6 h after memory reactivation disrupted contextual fear memory expression. Second, we observed that expression of a 30-day-old generalized contextual fear memory in a novel context was not affected by memory reactivation 6 h earlier. However, intra-ACC CNQX infusion before testing impaired contextual fear expression in the novel context, but not the original training context. Together, these data suggest that although the dorsal hippocampus may be recruited during systems reconsolidation, the ACC remains necessary for the expression of generalized contextual fear memory.

A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system.

Science.gov (United States)

Sudomoina, Marina; Latypova, Ekaterina; Favorova, Olga O; Golemis, Erica A; Serebriiskii, Ilya G

2004-04-29

Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the ability to achieve finely controlled regulation of gene expression. Previous efforts to achieve this goal have explored inducible drug regulation of gene expression, and construction of synthetic promoters based on two-hybrid paradigms, among others. In this report, we describe the combination of dimerizer-regulated two-hybrid and tetracycline regulatory elements in an ordered cascade, placing expression of endpoint reporters under the control of two distinct drugs. In this Dual Drug Control (DDC) system, a first plasmid expresses fusion proteins to DBD and AD, which interact only in the presence of a small molecule dimerizer; a second plasmid encodes a cassette transcriptionally responsive to the first DBD, directing expression of the Tet-OFF protein; and a third plasmid encodes a reporter gene transcriptionally responsive to binding by Tet-OFF. We evaluate the dynamic range and specificity of this system in comparison to other available systems. This study demonstrates the feasibility of combining two discrete drug-regulated expression systems in a temporally sequential cascade, without loss of dynamic range of signal induction. The efficient layering of control levels allowed by this combination of elements provides the potential for the generation of complex control circuitry that may advance ability to regulate gene expression in vivo.

A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC system

Directory of Open Access Journals (Sweden)

Golemis Erica A

2004-04-01

Full Text Available Abstract Background Whether for cell culture studies of protein function, construction of mouse models to enable in vivo analysis of disease epidemiology, or ultimately gene therapy of human diseases, a critical enabling step is the ability to achieve finely controlled regulation of gene expression. Previous efforts to achieve this goal have explored inducible drug regulation of gene expression, and construction of synthetic promoters based on two-hybrid paradigms, among others. Results In this report, we describe the combination of dimerizer-regulated two-hybrid and tetracycline regulatory elements in an ordered cascade, placing expression of endpoint reporters under the control of two distinct drugs. In this Dual Drug Control (DDC system, a first plasmid expresses fusion proteins to DBD and AD, which interact only in the presence of a small molecule dimerizer; a second plasmid encodes a cassette transcriptionally responsive to the first DBD, directing expression of the Tet-OFF protein; and a third plasmid encodes a reporter gene transcriptionally responsive to binding by Tet-OFF. We evaluate the dynamic range and specificity of this system in comparison to other available systems. Conclusion This study demonstrates the feasibility of combining two discrete drug-regulated expression systems in a temporally sequential cascade, without loss of dynamic range of signal induction. The efficient layering of control levels allowed by this combination of elements provides the potential for the generation of complex control circuitry that may advance ability to regulate gene expression in vivo.

Energy Systems High-Pressure Test Laboratory | Energy Systems Integration

Science.gov (United States)

Facility | NREL Energy Systems High-Pressure Test Laboratory Energy Systems High-Pressure Test Laboratory In the Energy Systems Integration Facility's High-Pressure Test Laboratory, researchers can safely test high-pressure hydrogen components. Photo of researchers running an experiment with a hydrogen fuel

Manual of a suite of computer codes, EXPRESS (EXact PREparedness Supporting System)

International Nuclear Information System (INIS)

Chino, Masamichi

1992-06-01

The emergency response supporting system EXPRESS (EXact PREparedness Supporting System) is constructed in JAERI for low cost engineering work stations under the UNIX operation. The purpose of this system is real-time predictions of affected areas due to radioactivities discharged into atmosphere from nuclear facilities. The computational models in EXPRESS are the mass-consistent wind field model EXPRESS-I and the particle dispersion model EXPRESS-II for atmospheric dispersions. In order to attain the quick response even when the codes are used in a small-scale computer, a high-speed iteration method MILUCR (Modified Incomplete Linear Unitary Conjugate Residual) is applied to EXPRESS-I and kernel density method is to EXPRESS-II. This manual describes the model configurations, code structures, related files, namelists and sample outputs of EXPRESS-I and -II. (author)

Evolution of a test article handling system for the SP-100 ground engineering system test

International Nuclear Information System (INIS)

Shen, E.J.; Schweiger, L.J.; Miller, W.C.; Gluck, R.; Devies, S.M.

1987-04-01

A simulated space environment test of a flight prototypic SP-100 reactor, control system, and flight shield will be conducted at the Hanford Engineering Development Laboratory (HEDL). The flight prototypic components and the supporting primary heat removal system are collectively known as the Nuclear Assembly Test Article (TA). The unique configuration and materials of fabrication for the Test Article require a specialized handling facility to support installation, maintenance, and final disposal operations. Westinghouse Hanford Company, the Test Site Operator, working in conjunction with General Electric Company, the Test Article supplier, developed and evaluated several handling concepts resulting in the selection of a reference Test Article Handling System. The development of the reference concept for the handling system is presented

Space Fission System Test Effectiveness

International Nuclear Information System (INIS)

Houts, Mike; Schmidt, Glen L.; Van Dyke, Melissa; Godfroy, Tom; Martin, James; Bragg-Sitton, Shannon; Dickens, Ricky; Salvail, Pat; Harper, Roger

2004-01-01

Space fission technology has the potential to enable rapid access to any point in the solar system. If fission propulsion systems are to be developed to their full potential, however, near-term customers need to be identified and initial fission systems successfully developed, launched, and utilized. One key to successful utilization is to develop reactor designs that are highly testable. Testable reactor designs have a much higher probability of being successfully converted from paper concepts to working space hardware than do designs which are difficult or impossible to realistically test. ''Test Effectiveness'' is one measure of the ability to realistically test a space reactor system. The objective of this paper is to discuss test effectiveness as applied to the design, development, flight qualification, and acceptance testing of space fission systems. The ability to perform highly effective testing would be particularly important to the success of any near-term mission, such as NASA's Jupiter Icy Moons Orbiter, the first mission under study within NASA's Project Prometheus, the Nuclear Systems Program

New tests to measure individual differences in matching and labelling facial expressions of emotion, and their association with ability to recognise vocal emotions and facial identity.

Science.gov (United States)

Palermo, Romina; O'Connor, Kirsty B; Davis, Joshua M; Irons, Jessica; McKone, Elinor

2013-01-01

Although good tests are available for diagnosing clinical impairments in face expression processing, there is a lack of strong tests for assessing "individual differences"--that is, differences in ability between individuals within the typical, nonclinical, range. Here, we develop two new tests, one for expression perception (an odd-man-out matching task in which participants select which one of three faces displays a different expression) and one additionally requiring explicit identification of the emotion (a labelling task in which participants select one of six verbal labels). We demonstrate validity (careful check of individual items, large inversion effects, independence from nonverbal IQ, convergent validity with a previous labelling task), reliability (Cronbach's alphas of.77 and.76 respectively), and wide individual differences across the typical population. We then demonstrate the usefulness of the tests by addressing theoretical questions regarding the structure of face processing, specifically the extent to which the following processes are common or distinct: (a) perceptual matching and explicit labelling of expression (modest correlation between matching and labelling supported partial independence); (b) judgement of expressions from faces and voices (results argued labelling tasks tap into a multi-modal system, while matching tasks tap distinct perceptual processes); and (c) expression and identity processing (results argued for a common first step of perceptual processing for expression and identity).

New Tests to Measure Individual Differences in Matching and Labelling Facial Expressions of Emotion, and Their Association with Ability to Recognise Vocal Emotions and Facial Identity

Science.gov (United States)

Palermo, Romina; O’Connor, Kirsty B.; Davis, Joshua M.; Irons, Jessica; McKone, Elinor

2013-01-01

Although good tests are available for diagnosing clinical impairments in face expression processing, there is a lack of strong tests for assessing “individual differences” – that is, differences in ability between individuals within the typical, nonclinical, range. Here, we develop two new tests, one for expression perception (an odd-man-out matching task in which participants select which one of three faces displays a different expression) and one additionally requiring explicit identification of the emotion (a labelling task in which participants select one of six verbal labels). We demonstrate validity (careful check of individual items, large inversion effects, independence from nonverbal IQ, convergent validity with a previous labelling task), reliability (Cronbach’s alphas of.77 and.76 respectively), and wide individual differences across the typical population. We then demonstrate the usefulness of the tests by addressing theoretical questions regarding the structure of face processing, specifically the extent to which the following processes are common or distinct: (a) perceptual matching and explicit labelling of expression (modest correlation between matching and labelling supported partial independence); (b) judgement of expressions from faces and voices (results argued labelling tasks tap into a multi-modal system, while matching tasks tap distinct perceptual processes); and (c) expression and identity processing (results argued for a common first step of perceptual processing for expression and identity). PMID:23840821

Differential expression of growth factors in irradiated mouse testes

International Nuclear Information System (INIS)

Mauduit, Claire; Siah, Ahmed; Foch, Marie; Chapet, Olivier; Clippe, Sebastien; Gerard, Jean-Pierre; Benahmed, Mohamed

2001-01-01

Purpose: By using as an experimental model the male mouse gonad, which contains both radiosensitive (germ) and radioresistant (somatic) cells, we have studied the growth factor (and/or receptor) expression of transforming growth factor-β receptor (TGFβ RI), stem cell factor (SCF), c-kit, Fas-L, Fas, tumor necrosis factor receptor (TNF R55), and leukemia inhibiting factor receptor (LIF-R) after local irradiation. Methods and Materials: Adult male mice were locally irradiated on the testes. Induction of apoptosis in the different testicular cell types following X-ray radiation was identified by the TdT-mediated dUTP Nick End Labeling (TUNEL) approach. Growth factor expression was evidenced by semiquantitative RT-PCR and Western blot analyses. Results: Apoptosis, identified through the TUNEL approach, occurred in radiosensitive testicular (premeotic) germ cells with the following kinetics: the number of apoptotic cells increased after 24 h (p<0.001) and was maximal 48 h after a 2-Gy ionizing radiation (p<0.001). Apoptotic cells were no longer observed 72 h after a 2-Gy irradiation. The number of apoptotic cells increased with the dose of irradiation (1-4 Gy). In the seminiferous tubules, the growth factor expression in premeiotic radiosensitive germ cells was modulated by irradiation. Indeed Fas, c-kit, and LIF-R expression, which occurs in (radiosensitive) germ cells, decreased 24 h after a 2-Gy irradiation, and the maximal decrease was observed with a 4-Gy irradiation. The decrease in Stra8 expression occurred earlier, at 4 h after a 2-Gy irradiation. In addition, a significant (p<0.03) decrease in Stra8 mRNA levels was observed at the lowest dose used (0.5 Gy, 48 h). Moreover, concerning a growth factor receptor, such as TGFβ RI, which is expressed both in radiosensitive and radioresistant cells, we observed a differential expression depending on the cell radiosensitivity after irradiation. Indeed, TGFβ RI expression was increased after irradiation in

GSMA: Gene Set Matrix Analysis, An Automated Method for Rapid Hypothesis Testing of Gene Expression Data

Directory of Open Access Journals (Sweden)

Chris Cheadle

2007-01-01

Full Text Available Background: Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The assignment of functional information to these complex patterns remains a challenging task in effectively interpreting data and correlating results from across experiments, projects and laboratories. Methods which allow the rapid and robust evaluation of multiple functional hypotheses increase the power of individual researchers to data mine gene expression data more efficiently.Results: We have developed (gene set matrix analysis GSMA as a useful method for the rapid testing of group-wise up- or downregulation of gene expression simultaneously for multiple lists of genes (gene sets against entire distributions of gene expression changes (datasets for single or multiple experiments. The utility of GSMA lies in its flexibility to rapidly poll gene sets related by known biological function or as designated solely by the end-user against large numbers of datasets simultaneously.Conclusions: GSMA provides a simple and straightforward method for hypothesis testing in which genes are tested by groups across multiple datasets for patterns of expression enrichment.

PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system

DEFF Research Database (Denmark)

Jansen, Anna M; Nässel, Dick R; Madsen, Kenneth L

2009-01-01

in the adult and larval Drosophila central nervous system. PICK1 was found in cell bodies in the subesophageal ganglion, the antennal lobe, the protocerebrum, and the neuroendocrine center pars intercerebralis. The cell types that express PICK1 were identified using GAL4 enhancer trap lines. The PICK1...... (AMPA) receptor subunit GluR2 and the dopamine transporter. PICK1 is strongly implicated in GluR2 trafficking and synaptic plasticity. In mammals, PICK1 has been characterized extensively in cell culture studies. To study PICK1 in an intact system, we characterized PICK1 expression immunohistochemically...... neurons in the neuroendocrine system, which express the transcription factor DIMM and the amidating enzyme peptidylglycine-alpha-hydroxylating monooxygenase (PHM). The PICK1-positive cells include neurosecretory cells that produce the insulin-like peptide dILP2. PICK1 expression in insulin-producing cells...

Heat flux expressions that satisfy the conservation laws in atomistic system involving multibody potentials

Energy Technology Data Exchange (ETDEWEB)

Fu, Yao, E-mail: Yao.Fu@colorado.edu; Song, Jeong-Hoon, E-mail: JH.Song@colorado.edu

2015-08-01

Heat flux expressions are derived for multibody potential systems by extending the original Hardy's methodology and modifying Admal & Tadmor's formulas. The continuum thermomechanical quantities obtained from these two approaches are easy to compute from molecular dynamics (MD) results, and have been tested for a constant heat flux model in two distinctive systems: crystalline iron and polyethylene (PE) polymer. The convergence criteria and affecting parameters, i.e. spatial and temporal window size, and specific forms of localization function are found to be different between the two systems. The conservation of mass, momentum, and energy are discussed and validated within this atomistic–continuum bridging.

Heat flux expressions that satisfy the conservation laws in atomistic system involving multibody potentials

International Nuclear Information System (INIS)

Fu, Yao; Song, Jeong-Hoon

2015-01-01

Heat flux expressions are derived for multibody potential systems by extending the original Hardy's methodology and modifying Admal & Tadmor's formulas. The continuum thermomechanical quantities obtained from these two approaches are easy to compute from molecular dynamics (MD) results, and have been tested for a constant heat flux model in two distinctive systems: crystalline iron and polyethylene (PE) polymer. The convergence criteria and affecting parameters, i.e. spatial and temporal window size, and specific forms of localization function are found to be different between the two systems. The conservation of mass, momentum, and energy are discussed and validated within this atomistic–continuum bridging

Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides)

Science.gov (United States)

Blum, Jason L.; Nyagode, Beatrice A.; James, Margaret O.; Denslow, Nancy D.

2010-01-01

The organochlorine pesticide methoxychlor (MXC) is an environmental estrogen known to stimulate the expression of the egg-yolk protein, vitellogenin (Vtg) in fish species. To begin to understand the underlying mechanisms for how MXC exerts its deleterious effects on the endocrine system, male largemouth bass (Micropterus salmoides) were treated with 2.5, 10, or 25 mg/kg MXC and compared to fish pair-treated with 1 mg/kg 17β-estradiol (E2), and vehicle control. Fish were sacrificed 24, 48, or 72 h following treatment. The liver and testes were then assayed for changes in expression of the three bass estrogen receptors (ERs α, βa, and βb) in tissues, as well as Vtg and cytochrome P450 (CYP) 3A isoform 68 in the liver and steroidogenic acute regulatory protein (StAR) in the testes. In the liver, significant increases in gene expression were seen for each of the genes measured by 24 h and each returned to the level of the vehicle by 72 h. Total testosterone 6β-hydroxylase activity, reflective of CYP3A activity, was also increased by 24 h for all of the exposures. In the testes, ERα was unaffected by any treatment, ERβa was upregulated only by MXC, peaking at 24 h for the 2.5 and 10 mg/kg MXC and at 48 h for the 25 mg/kg MXC treatment. By 72 h, the MXC effects had disappeared, while E2 significantly decreased the expression of ERβa mRNA. ERβb expression in the testes was stimulated by all concentrations of MXC by 24 h and the effect remained up to 72 h, whereas E2 had no effect. Finally, StAR expression was found to also be decreased by E2 and all MXC treatments. However, the effect on StAR expression by E2 occurred within 24 h, while the effect by all concentrations of MXC was not seen until 72 h after treatment. The stimulatory effects of E2 and 25 mg/kg MXC on the expression of the ERs in the liver were opposite to the responses seen in the testes, suggesting an inverted relationship between these two tissue types. These results provide a possible mechanism

Test results of HTTR control system

International Nuclear Information System (INIS)

Motegi, Toshihiro; Iigaki, Kazuhiko; Saito, Kenji; Sawahata, Hiroaki; Hirato, Yoji; Kondo, Makoto; Shibutani, Hideki; Ogawa, Satoru; Shinozaki, Masayuki; Mizushima, Toshihiko; Kawasaki, Kozo

2006-06-01

The plant control performance of the IHX helium flow rate control system, the PPWC helium flow rate control system, the secondary helium flow rate control system, the inlet temperature control system, the reactor power control system and the outlet temperature control system of the HTTR are obtained through function tests and power-up tests. As the test results, the control systems show stable control response under transient condition. Both of inlet temperature control system and reactor power control system shows stable operation from 30% to 100%, respectively. This report describes the outline of control systems and test results. (author)

Developing a new generation of breast cancer clinical gene expression tests.

Science.gov (United States)

Kos, Zuzana; Nielsen, Torsten O

2014-07-07

When treatment decisions are based purely on clinicopathological factors, many women with estrogen receptor-positive/human epidermal growth factor receptor 2-negative cancers are overtreated. Gene expression profiles are valuable clinical tools that stratify the recurrence risk to identify patients most likely to benefit from adjuvant systemic therapies. Building upon greater understanding of tumor biology and more rigorous approaches to validation (including independent studies with a high level of evidence), several second-generation multigene tests have been developed. In the previous issue, Martin and colleagues report the third clinical validation study for EndoPredict, a distributed assay to assess risk of distant recurrences in estrogen receptor-positive/human epidermal growth factor receptor 2-negative women. The authors confirm the assay's independent prognostic value in premenopausal and postmenopausal, node-positive women treated with contemporary chemotherapy followed by endocrine therapy. EndoPredict did not, however, predict benefit from adding paclitaxel. Predictive signatures for selecting among chemotherapy regimens remain an area needing further development.

Baculovirus expression vector system: An efficient tool for the ...

African Journals Online (AJOL)

Baculovirus expression vector system is considered one of the most successful and widely acceptable means for the production of recombinant proteins in extremely large quantities. Proper posttranslational modifications of the expressed proteins in insect cells, the usual host of baculoviruses, get them soluble, correctly ...

SALL4 expression in gonocytes and spermatogonial clones of postnatal mouse testes.

Directory of Open Access Journals (Sweden)

Kathrin Gassei

Full Text Available The spermatogenic lineage is established after birth when gonocytes migrate to the basement membrane of seminiferous tubules and give rise to spermatogonial stem cells (SSC. In adults, SSCs reside within the population of undifferentiated spermatogonia (A(undiff that expands clonally from single cells (A(single to form pairs (A(paired and chains of 4, 8 and 16 A(aligned spermatogonia. Although stem cell activity is thought to reside in the population of A(single spermatogonia, new research suggests that clone size alone does not define the stem cell pool. The mechanisms that regulate self-renewal and differentiation fate decisions are poorly understood due to limited availability of experimental tools that distinguish the products of those fate decisions. The pluripotency factor SALL4 (sal-like protein 4 is implicated in stem cell maintenance and patterning in many organs during embryonic development, but expression becomes restricted to the gonads after birth. We analyzed the expression of SALL4 in the mouse testis during the first weeks after birth and in adult seminiferous tubules. In newborn mice, the isoform SALL4B is expressed in quiescent gonocytes at postnatal day 0 (PND0 and SALL4A is upregulated at PND7 when gonocytes have colonized the basement membrane and given rise to spermatogonia. During steady-state spermatogenesis in adult testes, SALL4 expression overlapped substantially with PLZF and LIN28 in A(single, A(paired and A(aligned spermatogonia and therefore appears to be a marker of undifferentiated spermatogonia in mice. In contrast, co-expression of SALL4 with GFRα1 and cKIT identified distinct subpopulations of A(undiff in all clone sizes that might provide clues about SSC regulation. Collectively, these results indicate that 1 SALL4 isoforms are differentially expressed at the initiation of spermatogenesis, 2 SALL4 is expressed in undifferentiated spermatogonia in adult testes and 3 SALL4 co-staining with GFRα1 and c

Testing lifting systems in nuclear facilities

International Nuclear Information System (INIS)

Kling, H.; Laug, R.

1984-01-01

Lifting systems in nuclear facilities must be inspected at regular intervals after having undergone their first acceptance test. These inspections are frequently carried out by service firms which not only employ the skilled personnel required for such jobs but also make available the necessary test equipment. The inspections in particular include a number of sophisticated load tests for which test load systems have been developed to allow lifting systems to be tested so that reactor specific boundary conditions are taken into account. In view of the large number of facilities to be inspected, the test load system is a modular system. (orig.) [de

Inhalation method for delivery of nanoparticles to the Drosophila respiratory system for toxicity testing

International Nuclear Information System (INIS)

Posgai, Ryan; Ahamed, Maqusood; Hussain, Saber M.; Rowe, John J.; Nielsen, Mark G.

2009-01-01

The growth of the nanotechnology industry and subsequent proliferation of nanoparticle types present the need to rapidly assess nanoparticle toxicity. We present a novel, simple and cost-effective nebulizer-based method to deliver nanoparticles to the Drosophila melanogaster respiratory system, for the purpose of toxicity testing. FluoSpheres (registered) , silver, and CdSe/ZnS nanoparticles of different sizes were effectively aerosolized, showing the system is capable of functioning with a wide range of nanoparticle types and sizes. Red fluorescent CdSe/ZnS nanoparticles were successfully delivered to the fly respiratory system, as visualized by fluorescent microscopy. Silver coated and uncoated nanoparticles were delivered in a toxicity test, and induced Hsp70 expression in flies, confirming the utility of this model in toxicity testing. This is the first method developed capable of such delivery, provides the advantage of the Drosophila health model, and can serve as a link between tissue culture and more expensive mammalian models in a tiered toxicity testing strategy.

Inhalation method for delivery of nanoparticles to the Drosophila respiratory system for toxicity testing

Energy Technology Data Exchange (ETDEWEB)

Posgai, Ryan; Ahamed, Maqusood [Department of Biology, University of Dayton, Dayton, OH, 45469-2320 (United States); Hussain, Saber M. [Applied Biotechnology Branch, Human Effectiveness Directorate Air Force Research Laboratory/RHBP, Wright-Patterson Air Force Base, OH, 45433 (United States); Rowe, John J. [Department of Biology, University of Dayton, Dayton, OH, 45469-2320 (United States); Nielsen, Mark G., E-mail: Mark.Nielsen@notes.udayton.edu [Department of Biology, University of Dayton, Dayton, OH, 45469-2320 (United States)

2009-12-20

The growth of the nanotechnology industry and subsequent proliferation of nanoparticle types present the need to rapidly assess nanoparticle toxicity. We present a novel, simple and cost-effective nebulizer-based method to deliver nanoparticles to the Drosophila melanogaster respiratory system, for the purpose of toxicity testing. FluoSpheres (registered) , silver, and CdSe/ZnS nanoparticles of different sizes were effectively aerosolized, showing the system is capable of functioning with a wide range of nanoparticle types and sizes. Red fluorescent CdSe/ZnS nanoparticles were successfully delivered to the fly respiratory system, as visualized by fluorescent microscopy. Silver coated and uncoated nanoparticles were delivered in a toxicity test, and induced Hsp70 expression in flies, confirming the utility of this model in toxicity testing. This is the first method developed capable of such delivery, provides the advantage of the Drosophila health model, and can serve as a link between tissue culture and more expensive mammalian models in a tiered toxicity testing strategy.

Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms

DEFF Research Database (Denmark)

Kristensen, D.M.; Nielsen, J.E.; Skakkebaek, N.E.

2008-01-01

NANOG and OCT-3/4, UTF-1 and REX-1 are expressed throughout human testes development. The expression pattern indicated that UTF-1 plays a possible role in spermatogonial self-renewal, whereas expression of REX-1 in meiotic cells from both testes and ovary indicate a role in meiosis. UFT-1 and REX-1...... and REX-1 during human gonadal development and in TGCT. METHODS: Expression of UTF-1 and REX-1 was studied in 52 specimens from human gonadal development and in 86 samples from TGCT. RESULTS: UTF-1 and REX-1 were expressed throughout male gonadal development. In the mature testis, UTF-1 was expressed...

Construction of a novel, stable, food-grade expression system by engineering the endogenous toxin-antitoxin system in Bacillus subtilis.

Science.gov (United States)

Yang, Sen; Kang, Zhen; Cao, Wenlong; Du, Guocheng; Chen, Jian

2016-02-10

Bacillus subtilis as an important workhorse that has been widely used to produce enzymes and metabolites. To broaden its applications, especially in the food and feed industry, we constructed a novel, stable, food-grade expression system by engineering its type II toxin-antitoxin system. The expression of the toxin EndoA, encoded by the chromosomal ydcE gene, was regulated by an endogenous, xylose-inducible promoter, while the ydcD gene, which encodes the unstable antitoxin EndoB, was inserted into a food-grade vector backbone, where its expression was driven by the native, constitutive promoter PylxM. By maintaining the xylose concentration above 2.0 g L(-1), this auto-regulated expression system was absolutely stable after 100 generations. Compared with traditional antibiotic-dependent expression systems, this novel expression system resulted in greater biomass and higher titers of desired products (enzymes or metabolites). Our results demonstrate that this stable, food-grade expression system is suitable for enzyme production and pathway engineering, especially for the production of food-grade enzymes and metabolites. Copyright © 2015 Elsevier B.V. All rights reserved.

Undifferentiated embryonic cell transcription factor 1 (UTF1) and deleted in azoospermia-like (DAZL) expression in the testes of donkeys.

Science.gov (United States)

Lee, Y S; Jung, H J; Yoon, M J

2017-04-01

Putative markers for each specific germ cell stage can be a useful tool to study the fate and functions of these cells. Undifferentiated embryonic cell transcription factor 1 (UTF1) is a putative marker for undifferentiated spermatogonia in humans, rats and horses. The deleted in azoospermia-like (DAZL) protein is also expressed by differentiated spermatogonia and primary spermatocytes in several species. However, whether the expression patterns of these molecular markers are identical and applicable to donkeys remains to be elucidated. The objective of this study was to investigate the expression patterns of UTF1 and DAZL in donkey testicular tissue, using immunohistochemistry (IHC). Testicular samples were collected from routine field castration of donkeys in Korea. The reproductive stages (pre- or post-puberty) of the testes were determined from the morphological characteristics of cross-sections of the seminiferous tubules. For IHC, the UTF1 and DAZL primary antibodies were diluted at 1:100 and 1:200, respectively. The immunolabelling revealed that UTF1 was expressed in approximately 50% of spermatogonia in the pre-pubertal stage, whereas its expression was limited to an early subset of spermatogonia in the post-pubertal stage. DAZL was expressed in some, but not all, spermatogonia in the pre-pubertal spermatogonia, and interestingly, its expression was also observed in spermatogonia and primary spermatocytes in the post-pubertal stage. Co-immunolabelling of the germ cells with both UTF1 and DAZL revealed three types of protein expression patterns at both reproductive stages, namely UTF1 only, DAZL only and both UTF1 and DAZL. These protein molecules were not expressed in Sertoli and Leydig cells. In conclusion, a co-immunolabelling system with UTF1 and DAZL antibodies may be used to identify undifferentiated (UTF1 only), differentiating (UTF1 and DAZL), and differentiated spermatogonia (DAZL only) in donkey testes. © 2017 Blackwell Verlag GmbH.

Expression of group III metabotropic glutamate receptors in the reproductive system of male mice.

Science.gov (United States)

Marciniak, Marcin; Chruścicka, Barbara; Lech, Tomasz; Burnat, Grzegorz; Pilc, Andrzej

2016-03-01

Although the presence of metabotropic glutamate (mGlu) receptors in the central nervous system is well documented, they have recently been found in peripheral and non-neuronal tissues. In the present study we investigated the expression of group III mGlu receptors in the reproductive system of male mice. Reverse transcription-polymerase chain reaction analysis revealed the presence of mGlu6, mGlu7 and mGlu8 (but not mGlu4) receptor transcripts in testes and epididymides from adult mice. In addition, expression of mGlu6 (Grm6) and mGlu8 receptor (Grm8) mRNA was detected in spermatozoa isolated from the vas deferens. The vas deferens was found to contain only mGlu7 receptor (Grm7) mRNA, which was particularly intense in 21-day-old male mice. In penile homogenates, only the mGlu7 receptor signal was detected. Genetic ablation of the mGlu7 receptor in males led to fertility disorders manifested by decreased insemination capability as well as deterioration of sperm parameters, particularly sperm motility, vitality, sperm membrane integrity and morphology, with a simultaneous increase in sperm concentration. These results indicate that constitutively expressed mGlu receptors in the male reproductive system may play an important role in ejaculation and/or erection processes, as well as in the formation and maturation of spermatozoa.

Built-In Data-Flow Integration Testing in Large-Scale Component-Based Systems

Science.gov (United States)

Piel, Éric; Gonzalez-Sanchez, Alberto; Gross, Hans-Gerhard

Modern large-scale component-based applications and service ecosystems are built following a number of different component models and architectural styles, such as the data-flow architectural style. In this style, each building block receives data from a previous one in the flow and sends output data to other components. This organisation expresses information flows adequately, and also favours decoupling between the components, leading to easier maintenance and quicker evolution of the system. Integration testing is a major means to ensure the quality of large systems. Their size and complexity, together with the fact that they are developed and maintained by several stake holders, make Built-In Testing (BIT) an attractive approach to manage their integration testing. However, so far no technique has been proposed that combines BIT and data-flow integration testing. We have introduced the notion of a virtual component in order to realize such a combination. It permits to define the behaviour of several components assembled to process a flow of data, using BIT. Test-cases are defined in a way that they are simple to write and flexible to adapt. We present two implementations of our proposed virtual component integration testing technique, and we extend our previous proposal to detect and handle errors in the definition by the user. The evaluation of the virtual component testing approach suggests that more issues can be detected in systems with data-flows than through other integration testing approaches.

Minicomputer controlled test system for process control and monitoring systems

International Nuclear Information System (INIS)

Worster, L.D.

A minicomputer controlled test system for testing process control and monitoring systems is described. This system, in service for over one year, has demonstrated that computerized control of such testing has a real potential for expanding the scope of the testing, improving accuracy of testing, and significantly reducing the time required to do the testing. The test system is built around a 16-bit minicomputer with 12K of memory. The system programming language is BASIC with the addition of assembly level routines for communication with the peripheral devices. The peripheral devices include a 100 channel scanner, analog-to-digital converter, visual display, and strip printer. (auth)

Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system.

Science.gov (United States)

Cheng, L; Chen, G; Ding, G; Zhao, Z; Dong, T; Hu, Z

2015-04-27

The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a pucC overexpression strain, a pucC overexpression vector was constructed and transformed into R. sphaeroides CQU68. The overexpression efficiency was evaluated by quantitative real-time polymerase chain reaction. A well-used reporter β-glucuronidase (GUS) was fusion-expressed with LH2 to evaluate the heterologous protein expression level. As a result, the cell culture and protein in the pucC overexpression strain showed much higher typical spectral absorption peaks at 800 and 850 nm compared with the non-overexpression strain, suggesting a higher expression level of LH2-GUS fusion protein in the pucC overexpression strain. This result was further confirmed by Western blot, which also showed a much higher level of heterologous protein expression in the pucC overexpression strain. We further compared GUS activity in pucC overexpression and non-overexpression strains, the results of which showed that GUS activity in the pucC overexpression strain was approximately ten-fold that in the non-overexpression strain. These results demonstrate that overexpressed pucC can promote heterologous protein expression levels in R. sphaeroides.

Expression of monellin in a food-grade delivery system in Saccharomyces cerevisiae.

Science.gov (United States)

Liu, Jun; Yan, Da-zhong; Zhao, Sheng-jun

2015-10-01

Genetically modified (GM) foods have caused much controversy. Construction of a food-grade delivery system is a desirable technique with presumptive impact on industrial applications from the perspective of bio-safety. The aim of this study was to construct a food-grade delivery system for Saccharomyces cerevisiae and to study the expression of monellin from the berries of the West African forest plant Dioscoreophyllum cumminsii in this system. A food-grade system for S. cerevisiae was constructed based on ribosomal DNA (rDNA)-mediated homologous recombination to enable high-copy-number integration of the expression cassette inserted into the rDNA locus. A copper resistance gene (CUP1) was used as the selection marker for yeast transformation. Because variants of transformants containing different copy numbers at the CUP1 locus can be readily selected after growth in the presence of elevated copper levels, we suggest that this system would prove useful in the generation of tandemly iterated gene clusters. Using this food-grade system, a single-chain monellin gene was heterologously expressed. The yield of monellin reached a maximum of 675 mg L(-1) . This system harbors exclusively S. cerevisiae DNA with no antibiotic resistance genes, and it should therefore be appropriate for safe use in the food industry. Monellin was shown to be expressed in this food-grade delivery system. To our knowledge, this is the first report so far on expression of monellin in a food-grade expression system in S. cerevisiae. © 2014 Society of Chemical Industry.

Iterative Cellular Screening System for Nanoparticle Safety Testing

Directory of Open Access Journals (Sweden)

Franziska Sambale

2015-01-01

Full Text Available Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.

Using heterologous expression systems to characterize potassium and sodium transport activities.

Science.gov (United States)

Rodríguez, Alonso; Benito, Begoña; Cagnac, Olivier

2012-01-01

The expression of plant transporters in simple well-characterized cell systems is an irreplaceable technique for gaining insights into the kinetic and energetic features of plant transporters. Among all the available expression systems, yeast cells offer the highest simplicity and have the capacity to mimic the in vivo properties of plant transporters. Here, we describe the use of yeast mutants to express K(+) and Na(+) plant transporters and discuss some experimental problems that can produce misleading results.

Fatigue tests of dowel-socket systems

International Nuclear Information System (INIS)

Chiang, D.D.

1976-01-01

A test program was conducted to determine the fatigue behavior of LHTGR fuel element dowel/socket systems. Two dowel/socket systems, namely, a four-dowel system and a five-dowel system, were tested to failure under shear loads applied through a fatigue test apparatus to simulate repetitive loading during a seismic event

System Performance and Testing

NARCIS (Netherlands)

Frei, U.; Oversloot, H.

2004-01-01

This chapter compares and contrasts the system performance of two widely used solar thermal systems using testing and simulation programs. Solar thermal systems are used in many countries for heating domestically used water. In addition to the simple thermosiphon systems, better designed pumped

Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.

Science.gov (United States)

Baril, Patrick; Pichon, Chantal

2016-01-01

MicroRNAs (miRNAs) are a class of small, noncoding RNAs which regulate gene expression by directing their target mRNA for degradation or translational repression. Since their discovery in the early 1990s, miRNAs have emerged as key components in the posttranscriptional regulation of gene networks, shaping many biological processes from development, morphogenesis, differentiation, proliferation and apoptosis. Although understanding of the molecular basis of miRNA biology is improving, methods to monitor the dynamic and the spatiotemporal aspects of miRNA expression under physiopathological conditions are required. However, monitoring of miRNAs is difficult due to their small size, low abundance, high degree of sequence similarity, and their dynamic expression pattern which is subjected to tight transcriptional and post-transcriptional controls. Recently, we developed a miRNA monitoring system called RILES, standing for RNAi-inducible expression system, which relies on an engineered regulatable expression system, to switch on the expression of the luciferase gene when the targeted miRNA is expressed in cells. We demonstrated that RILES is a specific, sensitive, and robust method to determine the fine-tuning of miRNA expression during the development of an experimental pathological process in mice. Because RILES offers the possibility for longitudinal studies on individual subjects, sharper insights into miRNA regulation can be generated, with applications in physiology, pathophysiology and development of RNAi-based therapies. This chapter describes methods and protocols to monitor the expression of myomiR-206, -1, and -133 in the tibialis anterior muscle of mice. These protocols can be used and adapted to monitor the expression of other miRNAs in other biological processes.

Benefits of expressive writing in reducing test anxiety: A randomized controlled trial in Chinese samples.

Science.gov (United States)

Shen, Lujun; Yang, Lei; Zhang, Jing; Zhang, Meng

2018-01-01

To explore the effect of expressive writing of positive emotions on test anxiety among senior-high-school students. The Test Anxiety Scale (TAS) was used to assess the anxiety level of 200 senior-high-school students. Seventy-five students with high anxiety were recruited and divided randomly into experimental and control groups. Each day for 30 days, the experimental group engaged in 20 minutes of expressive writing of positive emotions, while the control group was asked to merely write down their daily events. A second test was given after the month-long experiment to analyze whether there had been a reduction in anxiety among the sample. Quantitative data was obtained from TAS scores. The NVivo10.0 software program was used to examine the frequency of particular word categories used in participants' writing manuscripts. Senior-high-school students indicated moderate to high test anxiety. There was a significant difference in post-test results (P 0.05). Students' writing manuscripts were mainly encoded on five code categories: cause, anxiety manifestation, positive emotion, insight and evaluation. There was a negative relation between positive emotion, insight codes and test anxiety. There were significant differences in the positive emotion, anxiety manifestation, and insight code categories between the first 10 days' manuscripts and the last 10 days' ones. Long-term expressive writing of positive emotions appears to help reduce test anxiety by using insight and positive emotion words for Chinese students. Efficient and effective intervention programs to ease test anxiety can be designed based on this study.

Benefits of expressive writing in reducing test anxiety: A randomized controlled trial in Chinese samples.

Directory of Open Access Journals (Sweden)

Lujun Shen

Full Text Available To explore the effect of expressive writing of positive emotions on test anxiety among senior-high-school students.The Test Anxiety Scale (TAS was used to assess the anxiety level of 200 senior-high-school students. Seventy-five students with high anxiety were recruited and divided randomly into experimental and control groups. Each day for 30 days, the experimental group engaged in 20 minutes of expressive writing of positive emotions, while the control group was asked to merely write down their daily events. A second test was given after the month-long experiment to analyze whether there had been a reduction in anxiety among the sample. Quantitative data was obtained from TAS scores. The NVivo10.0 software program was used to examine the frequency of particular word categories used in participants' writing manuscripts.Senior-high-school students indicated moderate to high test anxiety. There was a significant difference in post-test results (P 0.05. Students' writing manuscripts were mainly encoded on five code categories: cause, anxiety manifestation, positive emotion, insight and evaluation. There was a negative relation between positive emotion, insight codes and test anxiety. There were significant differences in the positive emotion, anxiety manifestation, and insight code categories between the first 10 days' manuscripts and the last 10 days' ones.Long-term expressive writing of positive emotions appears to help reduce test anxiety by using insight and positive emotion words for Chinese students. Efficient and effective intervention programs to ease test anxiety can be designed based on this study.

Benefits of expressive writing in reducing test anxiety: A randomized controlled trial in Chinese samples

Science.gov (United States)

Zhang, Jing; Zhang, Meng

2018-01-01

Purpose To explore the effect of expressive writing of positive emotions on test anxiety among senior-high-school students. Methods The Test Anxiety Scale (TAS) was used to assess the anxiety level of 200 senior-high-school students. Seventy-five students with high anxiety were recruited and divided randomly into experimental and control groups. Each day for 30 days, the experimental group engaged in 20 minutes of expressive writing of positive emotions, while the control group was asked to merely write down their daily events. A second test was given after the month-long experiment to analyze whether there had been a reduction in anxiety among the sample. Quantitative data was obtained from TAS scores. The NVivo10.0 software program was used to examine the frequency of particular word categories used in participants’ writing manuscripts. Results Senior-high-school students indicated moderate to high test anxiety. There was a significant difference in post-test results (P 0.05). Students’ writing manuscripts were mainly encoded on five code categories: cause, anxiety manifestation, positive emotion, insight and evaluation. There was a negative relation between positive emotion, insight codes and test anxiety. There were significant differences in the positive emotion, anxiety manifestation, and insight code categories between the first 10 days’ manuscripts and the last 10 days’ ones. Conclusions Long-term expressive writing of positive emotions appears to help reduce test anxiety by using insight and positive emotion words for Chinese students. Efficient and effective intervention programs to ease test anxiety can be designed based on this study. PMID:29401473

A powerful nonparametric method for detecting differentially co-expressed genes: distance correlation screening and edge-count test.

Science.gov (United States)

Zhang, Qingyang

2018-05-16

Differential co-expression analysis, as a complement of differential expression analysis, offers significant insights into the changes in molecular mechanism of different phenotypes. A prevailing approach to detecting differentially co-expressed genes is to compare Pearson's correlation coefficients in two phenotypes. However, due to the limitations of Pearson's correlation measure, this approach lacks the power to detect nonlinear changes in gene co-expression which is common in gene regulatory networks. In this work, a new nonparametric procedure is proposed to search differentially co-expressed gene pairs in different phenotypes from large-scale data. Our computational pipeline consisted of two main steps, a screening step and a testing step. The screening step is to reduce the search space by filtering out all the independent gene pairs using distance correlation measure. In the testing step, we compare the gene co-expression patterns in different phenotypes by a recently developed edge-count test. Both steps are distribution-free and targeting nonlinear relations. We illustrate the promise of the new approach by analyzing the Cancer Genome Atlas data and the METABRIC data for breast cancer subtypes. Compared with some existing methods, the new method is more powerful in detecting nonlinear type of differential co-expressions. The distance correlation screening can greatly improve computational efficiency, facilitating its application to large data sets.

'Fluorescent Cell Chip' for immunotoxicity testing: Development of the c-fos expression reporter cell lines

International Nuclear Information System (INIS)

Trzaska, Dominika; Zembek, Patrycja; Olszewski, Maciej; Adamczewska, Violetta; Ulleras, Erik; Dastych, JarosIaw

2005-01-01

The Fluorescent Cell Chip for in vitro immunotoxicity testing employs cell lines derived from lymphocytes, mast cells, and monocytes-macrophages transfected with various EGFP cytokine reporter gene constructs. While cytokine expression is a valid endpoint for in vitro immunotoxicity screening, additional marker for the immediate-early response gene expression level could be of interest for further development and refinement of the Fluorescent Cell Chip. We have used BW.5147.3 murine thymoma transfected with c-fos reporter constructs to obtain reporter cell lines expressing ECFP under the control of murine c-fos promoter. These cells upon serum withdrawal and readdition and incubation with heavy metal compounds showed paralleled induction of c-Fos expression as evidenced by Real-Time PCR and ECFP fluorescence as evidenced by computer-supported fluorescence microscopy. In conclusion, we developed fluorescent reporter cell lines that could be employed in a simple and time-efficient screening assay for possible action of chemicals on c-Fos expression in lymphocytes. The evaluation of usefulness of these cells for the Fluorescent Cell Chip-based detection of immunotoxicity will require additional testing with a larger number of chemicals

Orbital Express fluid transfer demonstration system

Science.gov (United States)

Rotenberger, Scott; SooHoo, David; Abraham, Gabriel

2008-04-01

Propellant resupply of orbiting spacecraft is no longer in the realm of high risk development. The recently concluded Orbital Express (OE) mission included a fluid transfer demonstration that operated the hardware and control logic in space, bringing the Technology Readiness Level to a solid TRL 7 (demonstration of a system prototype in an operational environment). Orbital Express (funded by the Defense Advanced Research Projects Agency, DARPA) was launched aboard an Atlas-V rocket on March 9th, 2007. The mission had the objective of demonstrating technologies needed for routine servicing of spacecraft, namely autonomous rendezvous and docking, propellant resupply, and orbital replacement unit transfer. The demonstration system used two spacecraft. A servicing vehicle (ASTRO) performed multiple dockings with the client (NextSat) spacecraft, and performed a variety of propellant transfers in addition to exchanges of a battery and computer. The fluid transfer and propulsion system onboard ASTRO, in addition to providing the six degree-of-freedom (6 DOF) thruster system for rendezvous and docking, demonstrated autonomous transfer of monopropellant hydrazine to or from the NextSat spacecraft 15 times while on orbit. The fluid transfer system aboard the NextSat vehicle was designed to simulate a variety of client systems, including both blowdown pressurization and pressure regulated propulsion systems. The fluid transfer demonstrations started with a low level of autonomy, where ground controllers were allowed to review the status of the demonstration at numerous points before authorizing the next steps to be performed. The final transfers were performed at a full autonomy level where the ground authorized the start of a transfer sequence and then monitored data as the transfer proceeded. The major steps of a fluid transfer included the following: mate of the coupling, leak check of the coupling, venting of the coupling, priming of the coupling, fluid transfer, gauging

Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule

Energy Technology Data Exchange (ETDEWEB)

Yagi, Hirokazu [Nagoya City University, Faculty and Graduate School of Pharmaceutical Sciences (Japan); Nakamura, Masatoshi [National Institute of Agrobiological Sciences, Genetic Resources Conservation Research Unit, Genetic Resources Center (Japan); Yokoyama, Jun [Taiyo Nippon Sanso Corporation, Tsukuba Laboratories (Japan); Zhang, Ying; Yamaguchi, Takumi [National Institutes of Natural Sciences, Institute for Molecular Science and Okazaki Institute for Integrative Bioscience (Japan); Kondo, Sachiko [Nagoya City University, Faculty and Graduate School of Pharmaceutical Sciences (Japan); Kobayashi, Jun [Yamaguchi University, Department of Biological and Environmental Sciences, Faculty of Agriculture (Japan); Kato, Tatsuya; Park, Enoch Y. [Shizuoka University, Laboratory of Biotechnology, Research Institute of Green Science and Technology (Japan); Nakazawa, Shiori [Nagoya University, Sugashima Marine Biological Laboratory, Graduate School of Science (Japan); Hashii, Noritaka; Kawasaki, Nana [National Institute of Health Sciences, Division of Biological Chemistry and Biologicals (Japan); Kato, Koichi, E-mail: kkato@phar.nagoya-cu.ac.jp [Nagoya City University, Faculty and Graduate School of Pharmaceutical Sciences (Japan)

2015-06-15

Silkworms serve as promising bioreactors for the production of recombinant proteins, including glycoproteins and membrane proteins, for structural and functional protein analyses. However, lack of methodology for stable isotope labeling has been a major deterrent to using this expression system for nuclear magnetic resonance (NMR) structural biology. Here we developed a metabolic isotope labeling technique using commercially available silkworm larvae. The fifth instar larvae were infected with baculoviruses for co-expression of recombinant human immunoglobulin G (IgG) as a test molecule, with calnexin as a chaperone. They were subsequently reared on an artificial diet containing {sup 15}N-labeled yeast crude protein extract. We harvested 0.1 mg of IgG from larva with a {sup 15}N-enrichment ratio of approximately 80 %. This allowed us to compare NMR spectral data of the Fc fragment cleaved from the silkworm-produced IgG with those of an authentic Fc glycoprotein derived from mammalian cells. Therefore, we successfully demonstrated that our method enables production of isotopically labeled glycoproteins for NMR studies.

PathMAPA: a tool for displaying gene expression and performing statistical tests on metabolic pathways at multiple levels for Arabidopsis

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Ma Ligeng

2003-11-01

Full Text Available Abstract Background To date, many genomic and pathway-related tools and databases have been developed to analyze microarray data. In published web-based applications to date, however, complex pathways have been displayed with static image files that may not be up-to-date or are time-consuming to rebuild. In addition, gene expression analyses focus on individual probes and genes with little or no consideration of pathways. These approaches reveal little information about pathways that are key to a full understanding of the building blocks of biological systems. Therefore, there is a need to provide useful tools that can generate pathways without manually building images and allow gene expression data to be integrated and analyzed at pathway levels for such experimental organisms as Arabidopsis. Results We have developed PathMAPA, a web-based application written in Java that can be easily accessed over the Internet. An Oracle database is used to store, query, and manipulate the large amounts of data that are involved. PathMAPA allows its users to (i upload and populate microarray data into a database; (ii integrate gene expression with enzymes of the pathways; (iii generate pathway diagrams without building image files manually; (iv visualize gene expressions for each pathway at enzyme, locus, and probe levels; and (v perform statistical tests at pathway, enzyme and gene levels. PathMAPA can be used to examine Arabidopsis thaliana gene expression patterns associated with metabolic pathways. Conclusion PathMAPA provides two unique features for the gene expression analysis of Arabidopsis thaliana: (i automatic generation of pathways associated with gene expression and (ii statistical tests at pathway level. The first feature allows for the periodical updating of genomic data for pathways, while the second feature can provide insight into how treatments affect relevant pathways for the selected experiment(s.

Investigation of the comparative test method of solar DHW system; Solar kyuto system no toitsuteki seino shiken hoho no chosa

Energy Technology Data Exchange (ETDEWEB)

Oishi, M; Noguchi, T [Japan Quality Assurance Organization, Tokyo (Japan)

1996-10-27

For the development of a unified performance test method for solar DHW (domestic hot water) systems, the Japan Quality Assurance Organization test was applied to three types. In an individual test, each specimen is exposed to irradiation from a solar simulator for 8 hours in a specified pattern. Upon completion of the 8-hour irradiation, hot water is taken from the specimen, the amount being 1.1 times as large as the heat accumulation tank capacity, for the measurement of the collected heat. The control of the heat medium circulation pump, flow rate, and density, all supposed to affect the heat collecting performance, are examined. Stable data, not subject to meteorological factors and high in reproducibility, is obtained, allowing a single test to represent the heat collecting performance. A system test continues for 24 hours, from 8 o`clock on this morning through 8 o`clock on the following morning, with water collected in a specified pattern. This test is suitable for examining the in-service performance of a solar hot water system including the auxiliary heat source. If temperature is not set properly in the auxiliary heat source before testing, the system performance coefficient and sun-dependency coefficient will not be expressed in a uniform manner. For a correct evaluation, measurements should be made only after a several days` uninterrupted operation for the removal of the adverse effect of the heat residual in the heat accumulation tank. 4 refs., 5 figs., 9 tabs.

High expression of testes-specific protease 50 is associated with poor prognosis in colorectal carcinoma.

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Lei Zheng

Full Text Available BACKGROUND: Testes-specific protease 50 (TSP50 is normally expressed in testes and abnormally expressed in breast cancer, but whether TSP50 is expressed in colorectal carcinoma (CRC and its clinical significance is unclear. We aimed to detect TSP50 expression in CRC, correlate it with clinicopathological factors, and assess its potential diagnostic and prognostic value. METHODOLOGY/PRINCIPAL FINDINGS: TSP50 mRNAs and proteins were detected in 7 CRC cell lines and 8 CRC specimens via RT-PCR and Western blot analysis. Immunohistochemical analysis of TSP50, p53 and carcinoembryonic antigen (CEA with tissue microarrays composed of 95 CRCs, 20 colorectal adenomas and 20 normal colorectal tissues were carried out and correlated with clinicopathological characteristics and disease-specific survival for CRC patients. There was no significant correlation between the expression levels of TSP50 and p53 (P = 0.751 or CEA (P = 0.663. Abundant expression of TSP50 protein was found in CRCs (68.4% while it was poorly expressed in colorectal adenomas and normal tissues (P<0.0001. Thus, CRCs can be distinguished from them with high specificity (92.5% and positive predictive value (PPV, 95.6%. The survival of CRC patients with high TSP50 expression was significantly shorter than that of the patients with low TSP50 expression (P = 0.010, specifically in patients who had early-stage tumors (stage I and II; P = 0.004. Multivariate Cox regression analysis indicated that high TSP50 expression was a statistically significant independent risk factor (hazard ratio  = 2.205, 95% CI = 1.214-4.004, P = 0.009. CONCLUSION: Our data demonstrate that TSP50 is a potential effective indicator of poor survival for CRC patients, especially for those with early-stage tumors.

Light Duty Utility Arm system pre-operational (cold test) test plan

International Nuclear Information System (INIS)

Bennett, K.L.

1995-01-01

The Light Duty Utility (LDUA) Cold Test Facility, located in the Hanford 400 Area, will be used to support cold testing (pre- operational tests) of LDUA subsystems. Pre-operational testing is composed of subsystem development testing and rework activities, and integrated system qualification testing. Qualification testing will be conducted once development work is complete and documentation is under configuration control. Operational (hot) testing of the LDUA system will follow the testing covered in this plan and will be covered in a separate test plan

Testing on air cleaning systems: Testing of the components in-place tests

International Nuclear Information System (INIS)

Billard, F.; Brion, J.

1967-01-01

The reliability of air cleaning systems is dependent on testing they are submitted to. Although in-place tests are the most important as they act as final tests upon achieved plants, component tests are necessary too. They allow detection of defective units before they are installed, partition of unit defects from mounting defects and they are more sensitive. For similar reasons, material teats are most useful. The various tests are described, about aerosol filters for one part, iodine trap for the other. The checked features are: materials nature, units sizes, efficiency, air resistance, flammability, humidity resistance, temperature resistance, adsorbent friability, etc... On iodine trapping systems, small check traps, working by-pass with the main trap are periodically subjected to efficiency test. This control allow to cut down the in-place tests frequency, particularly when poisoning from organic vapours is to be feared. (authors) [fr

Development and Testing of the Solar System Concept Inventory

Science.gov (United States)

Hornstein, Seth D.; Prather, E. E.; English, T. R.; Desch, S. M.; Keller, J. M.; Collaboration of Astronomy Teaching Scholars CATS

2011-01-01

Trying to assess if our students really understand the ideas we present in class can be difficult. Concept inventories are research-validated assessment tools that can provide us with data to better understand whether we are successful in the classroom. The idea for the Solar System Concept Inventory (SSCI) was born after realizing that no concept inventory currently available covered details regarding the formation and evolution of our solar system. Topics were selected by having faculty identify the key concepts they address when teaching about the solar system and interviewing students in order to identify common naive ideas and reasoning difficulties relating to these key topics. Beginning in fall of 2008, a national multi-institutional field test began which would eventually involve nearly 2500 students and 17 instructors from 10 different institutions. After each round of testing, a group of instructors from multiple institutions around the country worked together to analyze the data and revise or eliminate underperforming questions. Each question was examined using a combination of point biserial, percent correct on the pre-test, and item difficulty to determine if the question was properly differentiating student understanding while also ensuring the question was not too easy or too hard. In this talk, I will present an overall outline of the development of the SSCI as well as the final testing results. The final version of the SSCI can be found at http://casa.colorado.edu/ hornstei/ssci/. This material is based upon work supported by the National Science Foundation under Grant No. 0715517, a CCLI Phase III Grant for the Collaboration of Astronomy Teaching Scholars (CATS). Any findings expressed in this material are those of the authors and do not necessarily reflect the views of the NSF.

Xiaochaihutang attenuates depressive/anxiety-like behaviors of social isolation-reared mice by regulating monoaminergic system, neurogenesis and BDNF expression.

Science.gov (United States)

Ma, Jie; Wang, Fang; Yang, Jingyu; Dong, Yingxu; Su, Guangyue; Zhang, Kuo; Pan, Xing; Ma, Ping; Zhou, Tingshuo; Wu, Chunfu

2017-08-17

Xiaochaihutang (XCHT), as a classical herbal formula for the treatment of "Shaoyang syndrome" has been demonstrated to exert an antidepressant effect in multiple animal models of depression as shown in our previous studies. However, the effects of XCHT on social isolation (SI)-reared mice have not been investigated. This study aims to explore the effects of XCHT on depressive/anxiety-like behaviors of SI-reared mice, and its implicated mechanisms, including alterations in the monoaminergic system, neurogenesis and neurotrophin expression. Male C57 BL/6J mice (aged 4 weeks after weaning) were reared isolatedly for 8 weeks and XCHT (0.8, 2.3, 7.0g/kg) were given by gavage once a day. Forced swimming test (FST), tail suspension test (TST), open field test (OFT), elevated-plus maze test (EPM) and intruder-induced aggression test were used to explore the effects of XCHT on depressive/anxiety-like behaviors of SI-reared mice after administration of XCHT for 6 weeks. HPLC-MS/MS was performed to quantify the levels of neurotransmitters in the hippocampus by in vivo microdialysis, while western immunoblotting was used to evaluate the action of XCHT on the synthesis, transport and degradation of monoamine neurotransmitters. Immunofluorescence was used to study the effects of XCHT on neurogenesis and neurotrophin expression, including Ki-67, DCX, BrdU and BDNF. Our results showed that administration of XCHT (0.8, 2.3 and 7.0g/kg) for 6 weeks significantly attenuated the increase in immobility time in TST and FST, improved the anxiety-like behaviors in OFT and EPM, and improved the aggressive behaviors of SI-reared mice. XCHT significantly elevated monoamine neurotransmitters levels and inhibited 5-HT turnover (5-HIAA/5-HT) in hippocampal microdialysates of SI-reared mice. In addition, we found XCHT enhanced monoamine neurotransmitter synthesis enzymes (TPH2 and TH) expressions, inhibited serotonin transporter (SERT) expression and decreased monoamine neurotransmitter

Safety testing for LHC access system

CERN Document Server

Valentini, F; Ninin, P; Scibile, S

2008-01-01

In the domain of Safety Real-Time Systems the problem of testing represents always a big effort in terms of time, costs and efficiency to guarantee an adequate coverage degree. Exhaustive tests may, in fact, not be practicable for large and distributed systems. This paper describes the testing process followed during the validation of the CERN's LHC Access System [1], responsible for monitoring and preventing physical risks for the personnel accessing the underground areas. In the paper we also present a novel strategy for the testing problem, intended to drastically reduce the time for the test patterns generation and execution. In particular, we propose a methodology for blackbox testing that relies on the application of Model Checking techniques. Model Checking is a formal method from computer science, commonly adopted to prove correctness of system’s models through an automatic system’s state space exploration against some property formulas.

The Expression of Markers for Intratubular Germ Cell Neoplasia in Normal Infantile Testes

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Kolja Kvist

2018-06-01

Full Text Available BackgroundPositive immunohistochemical expression of testicular cancer markers is often reported beyond 12 months of age in cryptorchid testes, which is assumed to indicate delayed maturation of the fetal germ cells, or neoplastic changes. These findings allowed for questions as to the extent of positive reaction in normal testes. The aim of the study was to clarify the expression of these markers in a normal material up to 2 years.MethodsTesticular material from 69 boys aged 1–690 days, who died of causes with no association of testicular pathology. Histology sections were incubated with primary antibodies including anti-placental-like alkaline phosphatase (PLAP, anti-C-Kit, anti-D2–40, and anti-Oct3/4. The mean germ cell number per tubular transverse section (G/T was calculated based on the G/T of both testes of every boy.ResultsThe mean G/T declined through the 690 days. PLAP appeared stably expressed throughout the ages studied. The likelihood of a positive reaction for C-Kit waned with increasing age within the study period. Positive staining for D2–40 and Oct3/4 was demonstrated up to 6 and 9 months respectively.ConclusionUp to 1 or 2 years of age, normal infantile testes contain germ cells positive for the immunohistochemical markers commonly utilized to aid in the detection of testicular cancer. This finding supports the concept of germ cells undergoing a continuous maturational process in a heterogeneous fashion, and that this process is not complete by 2 years of age.

SP-100 nuclear assembly test: Test assembly functional requirements and system arrangement

International Nuclear Information System (INIS)

Fallas, T.T.; Gluck, R.; Motwani, K.; Clay, H.; O'Neill, G.

1991-01-01

This paper describes the functional requirements and the system that will be tested to validate the reactor, flight shield, and flight controller of the SP-100 Generic Flight System (GFS). The Nuclear Assembly Test (NAT) consists of the test article (SP-100 reactor with control devices and the flight shield) and its supporting systems. The NAT test assembly is being designed by GE. Westinghouse Hanford Company (WHC) is designing the test cell and vacuum vessel system that will contain the NAT test assembly (Renkey et al. 1989). Preliminary design reviews have been completed and the final design is under way

CMIF ECLS system test findings

Science.gov (United States)

Schunk, Richard G.; Carrasquillo, Robyn L.; Ogle, Kathyrn Y.; Wieland, Paul O.; Bagdigian, Robert M.

1989-01-01

During 1987 three Space Station integrated Environmental Control and Life Support System (ECLSS) tests were conducted at the Marshall Space Flight Center (MSFC) Core Module Integration Facility (CMIF) as part of the MSFC ECLSS Phase II test program. The three tests ranged in duration from 50 to 150 hours and were conducted inside of the CMIF module simulator. The Phase II partial integrated system test configuration consisted of four regenerative air revitalization subsystems and one regenerative water reclamation subsystem. This paper contains a discussion of results and lessons learned from the Phase II test program. The design of the Phase II test configuration and improvements made throughout the program are detailed. Future plans for the MSFC CMIF test program are provided, including an overview of planned improvements for the Phase III program.

High Power Alternator Test Unit (ATU) Electrical System Test

Science.gov (United States)

Birchenough, Arthur; Hervol, David

2007-01-01

The Alternator Test Unit (ATU) in the Lunar Power System Facility (LPSF) located at the NASA Glenn Research Center (GRC) in Cleveland, OH was used to simulate the operating conditions and evaluate the performance of the ATU and it s interaction with various LPSF components in accordance with the JIMO AC Power System Requirements. The testing was carried out at the breadboard development level. Results of these tests will be used for the development and validation of analytical models for performance and lifetime prediction.

Transient Expression of Lumbrokinase (PI239 in Tobacco (Nicotiana tabacum Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots

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Alexia Dickey

2017-01-01

Full Text Available Lumbrokinases, a group of fibrinolytic enzymes extracted from earthworm, have been widely used to prevent and treat various cardiovascular diseases. They specifically target fibrin to effectively degrade thrombi without major side effects. Plant expression systems are becoming potential alternative expression platforms for producing pharmaceutical proteins. In this work, a lumbrokinase (PI239 was produced from a plant system. Both wild-type (WT and plant codon-optimized (OP PI239 gene sequences were synthesized and cloned into a geminivirus-based single-vector DNA replicon system. Both vectors were independently expressed in tobacco (Nicotiana tabacum leaves transiently by agroinfiltration. Overexpressed PI239 resulted in sudden tissue necrosis 3 days after infiltration. Remaining proteins were purified through His-tag affinity chromatography and analyzed with SDS-PAGE and Western blot methods. Purified PI239 successfully degraded artificial fibrin with relative activity of 13,400 U/mg when compared with commercial lumbrokinase product. In vitro tests demonstrated that plant-derived PI239 dissolved human blood clots and that the plant expression system is capable of producing functional PI239.

Test Telemetry And Command System (TTACS)

Science.gov (United States)

Fogel, Alvin J.

1994-01-01

The Jet Propulsion Laboratory has developed a multimission Test Telemetry and Command System (TTACS) which provides a multimission telemetry and command data system in a spacecraft test environment. TTACS reuses, in the spacecraft test environment, components of the same data system used for flight operations; no new software is developed for the spacecraft test environment. Additionally, the TTACS is transportable to any spacecraft test site, including the launch site. The TTACS is currently operational in the Galileo spacecraft testbed; it is also being provided to support the Cassini and Mars Surveyor Program projects. Minimal personnel data system training is required in the transition from pre-launch spacecraft test to post-launch flight operations since test personnel are already familiar with the data system's operation. Additionally, data system components, e.g. data display, can be reused to support spacecraft software development; and the same data system components will again be reused during the spacecraft integration and system test phases. TTACS usage also results in early availability of spacecraft data to data system development and, as a result, early data system development feedback to spacecraft system developers. The TTACS consists of a multimission spacecraft support equipment interface and components of the multimission telemetry and command software adapted for a specific project. The TTACS interfaces to the spacecraft, e.g., Command Data System (CDS), support equipment. The TTACS telemetry interface to the CDS support equipment performs serial (RS-422)-to-ethernet conversion at rates between 1 bps and 1 mbps, telemetry data blocking and header generation, guaranteed data transmission to the telemetry data system, and graphical downlink routing summary and control. The TTACS command interface to the CDS support equipment is nominally a command file transferred in non-real-time via ethernet. The CDS support equipment is responsible for

Vitrification Facility integrated system performance testing report

International Nuclear Information System (INIS)

Elliott, D.

1997-01-01

This report provides a summary of component and system performance testing associated with the Vitrification Facility (VF) following construction turnover. The VF at the West Valley Demonstration Project (WVDP) was designed to convert stored radioactive waste into a stable glass form for eventual disposal in a federal repository. Following an initial Functional and Checkout Testing of Systems (FACTS) Program and subsequent conversion of test stand equipment into the final VF, a testing program was executed to demonstrate successful performance of the components, subsystems, and systems that make up the vitrification process. Systems were started up and brought on line as construction was completed, until integrated system operation could be demonstrated to produce borosilicate glass using nonradioactive waste simulant. Integrated system testing and operation culminated with a successful Operational Readiness Review (ORR) and Department of Energy (DOE) approval to initiate vitrification of high-level waste (HLW) on June 19, 1996. Performance and integrated operational test runs conducted during the test program provided a means for critical examination, observation, and evaluation of the vitrification system. Test data taken for each Test Instruction Procedure (TIP) was used to evaluate component performance against system design and acceptance criteria, while test observations were used to correct, modify, or improve system operation. This process was critical in establishing operating conditions for the entire vitrification process

Test plan: Brayton Isotope Power System Ground Demonstration System (BIPS-GDS)

International Nuclear Information System (INIS)

1976-01-01

The purpose of this test plan is to provide an overall outline of all testing to be accomplished on the GDS. Included in this test plan are administrative requirements, instrumentation accuracies, instrumentation, equipment definitions, system test setup, and facility installation. The test program will enable collection of sufficient data to establish material, component, and system design integrity. The data will also be used to establish and evaluate component and system performance and reliability characteristics, verification of proper system component integration prior to initiation of Phase II, and flight system (FS) development

Continuous Improvement in Battery Testing at the NASA/JSC Energy System Test Area

Science.gov (United States)

Boyd, William; Cook, Joseph

2003-01-01

The Energy Systems Test Area (ESTA) at the Lyndon B. Johnson Space Center in Houston, Texas conducts development and qualification tests to fulfill Energy System Division responsibilities relevant to ASA programs and projects. EST A has historically called upon a variety of fluid, mechanical, electrical, environmental, and data system capabilities spread amongst five full-service facilities to test human and human supported spacecraft in the areas of propulsion systems, fluid systems, pyrotechnics, power generation, and power distribution and control systems. Improvements at ESTA are being made in full earnest of offering NASA project offices an option to choose a thorough test regime that is balanced with cost and schedule constraints. In order to continue testing of enabling power-related technologies utilized by the Energy System Division, an especially proactive effort has been made to increase the cost effectiveness and schedule responsiveness for battery testing. This paper describes the continuous improvement in battery testing at the Energy Systems Test Area being made through consolidation, streamlining, and standardization.

Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell

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A.M. Al-Sulaiman

2017-11-01

Full Text Available Several prokaryotic and eukaryotic expression systems have been used for in vitro production of viruses’ proteins. However eukaryotic expression system was always the first choice for production of proteins that undergo post-translational modification such as glycosylation. Recombinant baculoviruses have been widely used as safe vectors to express heterologous genes in the culture of insect cells, but the manipulation involved in creating, titrating, and amplifying viral stocks make it time consuming and laborious. Therefore, to facilitate rapid expression in insect cell, a plasmid based expression system was used to express herpes simplex type 1 glycoprotein D (HSV-1 gD and varicella zoster glycoprotein E (VZV gE. Recombinant plasmids were generated, transfected into insect cells (SF9, and both glycoproteins were expressed 48 h post-infection. A protein with approximately molecular weight of 64-kDa and 98-kDa for HSV-1 gD and VZV gE respectively was expressed and confirmed by SDS. Proteins were detected in insect cells cytoplasm and outer membrane by immunofluorescence. The antigenicity and immunoreactivity of each protein were confirmed by immunoblot and ELISA. Results suggest that this system can be an alternative to the traditional baculovirus expression for small scale expression system in insect cells.

GeoMEx: Geographic Information System (GIS) Prototype for Mars Express Data

Science.gov (United States)

Manaud, N.; Frigeri, A.; Ivanov, A. B.

2013-09-01

Server for the web publishing module. Interfaces with existing GIS front-end software (such as QGIS, GRASS, ArcView, or OpenLayers) will be investigated and tested in a second phase. This prototype is primarily intended to be used by the Mars Express instruments teams in support to their scientific investigations. It will also be used by the mission Archive Scientist in support to the data validation and PSA interface requirements definition tasks. Depending on its success, this prototype might be used in the future to demonstrate the benefit of a GIS component integration to ESA's planetary science operations planning systems.

Testing Infrastructure for Operating System Kernel Development

DEFF Research Database (Denmark)

Walter, Maxwell; Karlsson, Sven

2014-01-01

Testing is an important part of system development, and to test effectively we require knowledge of the internal state of the system under test. Testing an operating system kernel is a challenge as it is the operating system that typically provides access to this internal state information. Multi......-core kernels pose an even greater challenge due to concurrency and their shared kernel state. In this paper, we present a testing framework that addresses these challenges by running the operating system in a virtual machine, and using virtual machine introspection to both communicate with the kernel...... and obtain information about the system. We have also developed an in-kernel testing API that we can use to develop a suite of unit tests in the kernel. We are using our framework for for the development of our own multi-core research kernel....

Development of a new biofidelity ranking system for anthropomorphic test devices.

Science.gov (United States)

Rhule, Heather H; Maltese, Matthew R; Donnelly, Bruce R; Eppinger, Rolf H; Brunner, Jill K; Bolte, John H

2002-11-01

A new biofidelity assessment system is being developed and applied to three side impact dummies: the WorldSID-alpha, the ES-2 and the SID-HIII. This system quantifies (1) the ability of a dummy to load a vehicle as a cadaver does, "External Biofidelity," and (2) the ability of a dummy to replicate those cadaver responses that best predict injury potential, "Internal Biofidelity." The ranking system uses cadaver and dummy responses from head drop tests, thorax and shoulder pendulum tests, and whole body sled tests. Each test condition is assigned a weight factor based on the number of human subjects tested to form the biomechanical response corridor and how well the biofidelity tests represent FMVSS 214, side NCAP (SNCAP) and FMVSS 201 Pole crash environments. For each response requirement, the cumulative variance of the dummy response relative to the mean cadaver response (DCV) and the cumulative variance of the mean cadaver response relative to the mean plus one standard deviation (CCV) are calculated. The ratio of DCV/CCV expresses how well the dummy response duplicates the mean cadaver response: a smaller ratio indicating better biofidelity. For each test condition, the square root is taken of each Response Comparison Value (DCV/CCV), and then these values are averaged and multiplied by the appropriate Test Condition Weight. The weighted and averaged comparison values are then summed and divided by the sum of the Test Condition Weights to obtain a rank for each body region. Each dummy obtains an overall rank for External Biofidelity and an overall rank for Internal Biofidelity comprised of an average of the ranks from each body region. Of the three dummies studied, the selected comparison test data indicate that the WorldSID-alpha prototype dummy demonstrated the best overall External Biofidelity although improvement is needed in all of the dummies to better replicate human kinematics. All three dummies estimate potential injury assessment with similar levels of

Development of Final Running Test System for Digital Systems

Energy Technology Data Exchange (ETDEWEB)

Lee, Kwang-Dae; Lee, Eui-Jong; Lim, Hee-Taek; Kim, Min-Seok [KHNP Central Research Institute, Daejeon (Korea, Republic of)

2016-10-15

In nuclear industry, the newly designed systems to upgrade are qualified to meet IEEE standards and the regulatory guidelines for their functions, performance and reliability requirements. Failure Mode and Effect Analysis, Fault Tree Analysis, and Hazard Analysis have been used to improve the reliability of the control system. To ensure the completeness of the software, the verification and validation processes are carried out during the development process. In spite of the many efforts depending on the analysis and procedures, there are limitations to improve the reliability. The lessons learned from the currently installed system failures show the incompleteness of the final integration test. The current point-to-point and logic-to-logic separate test procedures manually performed by the engineers can cause some procedures missed and have effects on the critical functions. The design processes of the digital systems are met in accordance with the international standards and regulatory guidelines. The lessons learned from the failures of the running digital systems showed the limitations of the current verification and validation efforts. The various improvements and attempts have been considered including the expert review processes and the completeness of the test. In this paper, the Final Running Test Method evaluating the completeness of the digital system using the control patterns and the Test System Architecture are proposed.

Development of Final Running Test System for Digital Systems

International Nuclear Information System (INIS)

Lee, Kwang-Dae; Lee, Eui-Jong; Lim, Hee-Taek; Kim, Min-Seok

2016-01-01

In nuclear industry, the newly designed systems to upgrade are qualified to meet IEEE standards and the regulatory guidelines for their functions, performance and reliability requirements. Failure Mode and Effect Analysis, Fault Tree Analysis, and Hazard Analysis have been used to improve the reliability of the control system. To ensure the completeness of the software, the verification and validation processes are carried out during the development process. In spite of the many efforts depending on the analysis and procedures, there are limitations to improve the reliability. The lessons learned from the currently installed system failures show the incompleteness of the final integration test. The current point-to-point and logic-to-logic separate test procedures manually performed by the engineers can cause some procedures missed and have effects on the critical functions. The design processes of the digital systems are met in accordance with the international standards and regulatory guidelines. The lessons learned from the failures of the running digital systems showed the limitations of the current verification and validation efforts. The various improvements and attempts have been considered including the expert review processes and the completeness of the test. In this paper, the Final Running Test Method evaluating the completeness of the digital system using the control patterns and the Test System Architecture are proposed

A/T test system for production line; Seisan line yo A/T test system

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-03-10

This paper introduces an A/T test system for production line made by Meidensha, Inc. Rates and technology of mounting auto-transmissions (A/T) in recent automobiles are improving year after year. A/T production line testers were fabricated and supplied, including those for European countries. This system has the following features: the system employs low inertia AC motor, and is capable of performing tests in close conditions to actual cars; an insulated gate bipolar transistor (IGBT) inverter was employed to achieve control accuracy improvement and noise reduction; high-velocity transient measurement is possible by using a computer system based on the WindowsNT; a solenoid current slant control was employed to respond to electronically controlled A/T; and noise and vibration shift feeling instrumentation can also be used on option. Number of supply is four sets of front engine/front wheel drive (FF) car A/T testers for overseas countries, and two sets of FF A/T tests for use in Japan. (NEDO)

The Bodily Expressive Action Stimulus Test (BEAST). Construction and Validation of a Stimulus Basis for Measuring Perception of Whole Body Expression of Emotions.

Science.gov (United States)

de Gelder, Beatrice; Van den Stock, Jan

2011-01-01

Whole body expressions are among the main visual stimulus categories that are naturally associated with faces and the neuroscientific investigation of how body expressions are processed has entered the research agenda this last decade. Here we describe the stimulus set of whole body expressions termed bodily expressive action stimulus test (BEAST), and we provide validation data for use of these materials by the community of emotion researchers. The database was composed of 254 whole body expressions from 46 actors expressing 4 emotions (anger, fear, happiness, and sadness). In all pictures the face of the actor was blurred and participants were asked to categorize the emotions expressed in the stimuli in a four alternative-forced-choice task. The results show that all emotions are well recognized, with sadness being the easiest, followed by fear, whereas happiness was the most difficult. The BEAST appears a valuable addition to currently available tools for assessing recognition of affective signals. It can be used in explicit recognition tasks as well as in matching tasks and in implicit tasks, combined either with facial expressions, with affective prosody, or presented with affective pictures as context in healthy subjects as well as in clinical populations.

Budget impact analysis of gene expression tests to aid therapy decisions for breast cancer patients in Germany.

Science.gov (United States)

Lux, M P; Nabieva, N; Hildebrandt, T; Rebscher, H; Kümmel, S; Blohmer, J-U; Schrauder, M G

2018-02-01

Many women with early-stage, hormone receptor-positive breast cancer may not benefit from adjuvant chemotherapy. Gene expression tests can reduce chemotherapy over- and undertreatment by providing prognostic information on the likelihood of recurrence and, with Oncotype DX, predictive information on chemotherapy benefit. These tests are currently not reimbursed by German healthcare payers. An analysis was conducted to evaluate the budget impact of gene expression tests in Germany. Costs of gene expression tests and medical and non-medical costs associated with treatment were assessed from healthcare payer and societal perspectives. Costs were estimated from data collected at a university hospital and were combined with decision impact data for Oncotype DX, MammaPrint, Prosigna and EndoPredict (EPclin). Changes in chemotherapy use and budget impact were evaluated over 1 year for 20,000 women. Chemotherapy was associated with substantial annual costs of EUR 19,003 and EUR 84,412 per therapy from the healthcare payer and societal perspective, respectively. Compared with standard care, only Oncotype DX was associated with cost savings to healthcare payers and society (EUR 5.9 million and EUR 253 million, respectively). Scenario analysis showed that both women at high clinical but low genomic risk and low clinical but high genomic risk were important contributors to costs. Oncotype DX was the only gene expression test that was estimated to reduce costs versus standard care in Germany. The reimbursement of Oncotype DX testing in standard clinical practice in Germany should be considered. Copyright © 2017 Elsevier Ltd. All rights reserved.

21 CFR 862.3610 - Methamphetamine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Methamphetamine test system. 862.3610 Section 862....3610 Methamphetamine test system. (a) Identification. A methamphetamine test system is a device intended to measure methamphetamine, a central nervous system stimulating drug, in serum, plasma, and urine...

Expression of manganese superoxide dismutase in rat blood, heart and brain during induced systemic hypoxia

Directory of Open Access Journals (Sweden)

Septelia I. Wanandi

2011-02-01

Full Text Available Background: Hypoxia results in an increased generation of ROS. Until now, little is known about the role of MnSOD - a major endogenous antioxidant enzyme - on the cell adaptation response against hypoxia. The aim of this study was to  determine the MnSOD mRNA expression and levels of specific activity in blood, heart and brain of rats during induced systemic hypoxia.Methods: Twenty-five male Sprague Dawley rats were subjected to systemic hypoxia in an hypoxic chamber (at 8-10% O2 for 0, 1, 7, 14 and 21 days, respectively. The mRNA relative expression of MnSOD was analyzed using Real Time RT-PCR. MnSOD specific activity was determined using xanthine oxidase inhibition assay.Results: The MnSOD mRNA relative expression in rat blood and heart was decreased during early induced systemic hypoxia (day 1 and increased as hypoxia continued, whereas the mRNA expression in brain was increased since day 1 and reached its maximum level at day 7. The result of MnSOD specific activity during early systemic hypoxia was similar to the mRNA expression. Under very late hypoxic condition (day 21, MnSOD specific activity in blood, heart and brain was significantly decreased. We demonstrate a positive correlation between MnSOD mRNA expression and specific activity in these 3 tissues during day 0-14 of induced systemic hypoxia. Furthermore, mRNA expression and specific activity levels in heart strongly correlate with those in blood.Conclusion: The MnSOD expression at early and late phases of induced systemic hypoxia is distinctly regulated. The MnSOD expression in brain differs from that in blood and heart revealing that brain tissue can  possibly survive better from induced systemic hypoxia than heart and blood. The determination of MnSOD expression in blood can be used to describe its expression in heart under systemic hypoxic condition. (Med J Indones 2011; 20:27-33Keywords: MnSOD, mRNA expression, ROS, specific activity, systemic hypoxia

Launch Control System Software Development System Automation Testing

Science.gov (United States)

Hwang, Andrew

2017-01-01

The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administration's (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This system requires high quality testing that will measure and test the capabilities of the system. For the past two years, the Exploration and Operations Division at Kennedy Space Center (KSC) has assigned a group including interns and full-time engineers to develop automated tests to save the project time and money. The team worked on automating the testing process for the SCCS GUI that would use streamed simulated data from the testing servers to produce data, plots, statuses, etc. to the GUI. The software used to develop automated tests included an automated testing framework and an automation library. The automated testing framework has a tabular-style syntax, which means the functionality of a line of code must have the appropriate number of tabs for the line to function as intended. The header section contains either paths to custom resources or the names of libraries being used. The automation library contains functionality to automate anything that appears on a desired screen with the use of image recognition software to detect and control GUI components. The data section contains any data values strictly created for the current testing file. The body section holds the tests that are being run. The function section can include any number of functions that may be used by the current testing file or any other file that resources it. The resources and body section are required for all test files; the data and function sections can be left empty if the data values and functions being used are from a resourced library or another file. To help equip the automation team with better tools, the Project Lead of the Automated Testing Team, Jason Kapusta, assigned the task to install and train an optical character recognition (OCR

Efficient Expression of Acetylcholine-Binding Protein from Aplysia californica in Bac-to-Bac System

Directory of Open Access Journals (Sweden)

Bo Lin

2014-01-01

Full Text Available The Bac-to-Bac baculovirus expression system can efficiently produce recombinant proteins, but the system may have to be optimized to achieve high-level expression for different candidate proteins. We reported here the efficient expression of acetylcholine-binding proteins from sea hares Aplysia californica (Ac-AChBP and a convenient method to monitor protein expression level in this expression system. Three key factors affecting expression of Ac-AChBP were optimized for maximizing the yield, which included the cell density, volume of the infecting baculovirus inoculums, and the culturing time of postinfection. We have found it to reach a high yield of ∼5 mg/L, which needs 55 h incubation after infection at the cell density of 2 × 106 cells/mL with an inoculum volume ratio of 1 : 100. The optimized expression system in this study was also applied for expressing another protein Ls-AChBP from Lymnaea stagnalis successfully. Therefore, this established method is helpful to produce high yields of AChBP proteins for X-ray crystallographic structural and functional studies.

Experimental fast reactor JOYO MK-III functional test. Primary auxiliary cooling system test

International Nuclear Information System (INIS)

Karube, Koji; Akagi, Shinji; Terano, Toshihiro; Onuki, Osamu; Ito, Hideaki; Aoki, Hiroshi; Odo, Toshihiro

2004-03-01

This paper describes the results of primary auxiliary cooling system, which were done as a part of JOYO MK-III function test. The aim of the tests was to confirm the operational performance of primary auxiliary EMP and the protection system including siphon breaker of primary auxiliary cooling system. The items of the tests were: (Test No.): (Test item). 1) SKS-117: EMP start up test. 2) SKS-118-1: EMP start up test when pony motor running. 3) SKS-121: Function test of siphon breaker. The results of the tests satisfied the required performance, and demonstrated successful operation of primary auxiliary cooling system. (author)

Algevir: An Expression System for Microalgae Based on Viral Vectors

Directory of Open Access Journals (Sweden)

Bernardo Bañuelos-Hernández

2017-06-01

Full Text Available The use of recombinant algae for the production of valuable compounds is opening promising biotechnological applications. However, the development of efficient expression approaches is still needed to expand the exploitation of microalgae in biotechnology. Herein, the concept of using viral expression vectors in microalgae was explored for the first time. An inducible geminiviral vector leading to Rep-mediated replication of the expression cassette allowed the production of antigenic proteins at high levels. This system, called Algevir, allows the production of complex viral proteins (GP1 from Zaire ebolavirus and bacterial toxin subunits (B subunit of the heat-labile Escherichia coli enterotoxin, which retained their antigenic activity. The highest achieved yield was 1.25 mg/g fresh biomass (6 mg/L of culture, which was attained 3 days after transformation. The Algevir system allows for a fast and efficient production of recombinant proteins, overcoming the difficulties imposed by the low yields and unstable expression patterns frequently observed in stably transformed microalgae at the nuclear level; as well as the toxicity of some target proteins.

Algevir: An Expression System for Microalgae Based on Viral Vectors

Science.gov (United States)

Bañuelos-Hernández, Bernardo; Monreal-Escalante, Elizabeth; González-Ortega, Omar; Angulo, Carlos; Rosales-Mendoza, Sergio

2017-01-01

The use of recombinant algae for the production of valuable compounds is opening promising biotechnological applications. However, the development of efficient expression approaches is still needed to expand the exploitation of microalgae in biotechnology. Herein, the concept of using viral expression vectors in microalgae was explored for the first time. An inducible geminiviral vector leading to Rep-mediated replication of the expression cassette allowed the production of antigenic proteins at high levels. This system, called Algevir, allows the production of complex viral proteins (GP1 from Zaire ebolavirus) and bacterial toxin subunits (B subunit of the heat-labile Escherichia coli enterotoxin), which retained their antigenic activity. The highest achieved yield was 1.25 mg/g fresh biomass (6 mg/L of culture), which was attained 3 days after transformation. The Algevir system allows for a fast and efficient production of recombinant proteins, overcoming the difficulties imposed by the low yields and unstable expression patterns frequently observed in stably transformed microalgae at the nuclear level; as well as the toxicity of some target proteins. PMID:28713333

BMFT-UPTF densitometer system test report

International Nuclear Information System (INIS)

Menkhaus, D.E.

1985-11-01

This report documents acceptance test results performed on the five Upper Plenum Test Facility (UPTF) three-beam densitometer systems and spare parts. The five densitometer systems are used on the UPTF four hot legs and broken cold leg to measure average chordal-beam densities. The primary objectives of the tests performed were: to verify all assemblies fit as designed (mechanical fitup); to ensure radiation levels met the criteria (<2.5 mR/h); to verify that design accuracy requirements were met (performance tests); and to verify proper operation of the densitometer systems (functional checks). 15 figs., 11 tabs

System Testing of Desktop and Web Applications

Science.gov (United States)

Slack, James M.

2011-01-01

We want our students to experience system testing of both desktop and web applications, but the cost of professional system-testing tools is far too high. We evaluate several free tools and find that AutoIt makes an ideal educational system-testing tool. We show several examples of desktop and web testing with AutoIt, starting with simple…

Test software for BESIII MDC electronics system

International Nuclear Information System (INIS)

Zhang Hongyu; Sheng Huayi; Zhu Haitao; Ji Xiaolu; Zhao Dongxu

2006-01-01

This paper presents the design of Test System Software for BESIII MDC Electronics. Two kinds of test systems, SBS VP7 based and PowerPC based systems, and their corresponding test software are introduced. The software is developed in LabVIEW 7.1 and Microsoft Visual C++ 6.0, some test functions of the software, as well as their user interfaces, are described in detail. The software has been applied in hardware debugging, performance test and long term stability test. (authors)

A novel bidirectional expression system for simultaneous expression of both the protein-coding genes and short hairpin RNAs in mammalian cells

International Nuclear Information System (INIS)

Hung, C.-F.; Cheng, T.-L.; Wu, R.-H.; Teng, C.-F.; Chang, W.-T.

2006-01-01

RNA interference (RNAi) is an extremely powerful and widely used gene silencing approach for reverse functional genomics and molecular therapeutics. In mammals, the conserved poly(ADP-ribose) polymerase 2 (PARP-2)/RNase P bidirectional control promoter simultaneously expresses both the PARP-2 protein and RNase P RNA by RNA polymerase II- and III-dependent mechanisms, respectively. To explore this unique bidirectional control system in RNAi-mediated gene silencing strategy, we have constructed two novel bidirectional expression vectors, pbiHsH1 and pbiMmH1, which contained the PARP-2/RNase P bidirectional control promoters from human and mouse, for simultaneous expression of both the protein-coding genes and short hairpin RNAs. Analyses of the dual transcriptional activities indicated that these two bidirectional expression vectors could not only express enhanced green fluorescent protein as a functional reporter but also simultaneously transcribe shLuc for inhibiting the firefly luciferase expression. In addition, to extend its utility for the establishment of inherited stable clones, we have also reconstructed this bidirectional expression system with the blasticidin S deaminase gene, an effective dominant drug resistance selectable marker, and examined both the selection and inhibition efficiencies in drug resistance and gene expression. Moreover, we have further demonstrated that this bidirectional expression system could efficiently co-regulate the functionally important genes, such as overexpression of tumor suppressor protein p53 and inhibition of anti-apoptotic protein Bcl-2 at the same time. In summary, the bidirectional expression vectors, pbiHsH1 and pbiMmH1, should provide a simple, convenient, and efficient novel tool for manipulating the gene function in mammalian cells

Preoperational test report, primary ventilation system

Energy Technology Data Exchange (ETDEWEB)

Clifton, F.T.

1997-11-04

This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

Preoperational test report, primary ventilation system

International Nuclear Information System (INIS)

Clifton, F.T.

1997-01-01

This represents a preoperational test report for Primary Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space filtered venting of tanks AY101, AY102, AZ101, AZ102. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

Operational test report, integrated system test (ventilation upgrade)

International Nuclear Information System (INIS)

HARTY, W.M.

1999-01-01

Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, AY102, AZ101, AZ102

Operational test report integrated system test (ventilation upgrade)

Energy Technology Data Exchange (ETDEWEB)

HARTY, W.M.

1999-10-05

Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, Ay102, AZ101, AZ102.

Ship Systems Survivability Test Site

Data.gov (United States)

Federal Laboratory Consortium — Area for testing survivability of shipboard systems to include electrical, communications, and fire suppression. Multipurpose test range for supporting gun firing,...

Internal testing of pipe systems with IRIS inspection system

International Nuclear Information System (INIS)

1986-01-01

The internal piping inspection system IRIS allows inside testing of pipes with an internal diameter of NW 70 as a minimum, and of any horizontal or vertical layout of the piping system. Visual testing is done by means of an integrated CCD video system with high resolution power. Technical data are given and examples of applications, in the German and English language. (DG) [de

Test results of six-month test of two water electrolysis systems

Science.gov (United States)

Mills, E. S.; Wells, G. W.

1972-01-01

The two water electrolysis systems used in the NASA space station simulation 90-day manned test of a regenerative life support system were refurbished as required and subjected to 26-weeks of testing. The two electrolysis units are both promising systems for oxygen and hydrogen generation and both needed extensive long-term testing to evaluate the performance of the respective cell design and provide guidance for further development. Testing was conducted to evaluate performance in terms of current, pressure, variable oxygen demands, and orbital simulation. An automatic monitoring system was used to record, monitor and printout performance data at one minute, ten minute or one-hour intervals. Performance data is presented for each day of system operation for each module used during the day. Failures are analyzed, remedial action taken to eliminate problems is discussed and recommendations for redesign for future space applications are stated.

System design description for the whole element furnace testing system

International Nuclear Information System (INIS)

Ritter, G.A.; Marschman, S.C.; MacFarlan, P.J.; King, D.A.

1998-05-01

This document provides a detailed description of the Hanford Spent Nuclear Fuel (SNF) Whole Element Furnace Testing System located in the Postirradiation Testing Laboratory G-Cell (327 Building). Equipment specifications, system schematics, general operating modes, maintenance and calibration requirements, and other supporting information are provided in this document. This system was developed for performing cold vacuum drying and hot vacuum drying testing of whole N-Reactor fuel elements, which were sampled from the 105-K East and K West Basins. The proposed drying processes are intended to allow dry storage of the SNF for long periods of time. The furnace testing system is used to evaluate these processes by simulating drying sequences with a single fuel element and measuring key system parameters such as internal pressures, temperatures, moisture levels, and off-gas composition

Excitation system testing in HPP 'Uvac'

Directory of Open Access Journals (Sweden)

Milojčić Nemanja

2011-01-01

Full Text Available The excitation system of hydro unit in HPP 'Uvac' and results of testings of excitation system performed for achieving of unit's mathematical model are presented in this paper. Description of excitation system equipment, parameters of regulators and results obtained after testings are presented. The presented results showed that the regulators are properly adjusted and that the excitation system is completely functional and reliable.

The Systems Test Architect: Enabling The Leap From Testable To Tested

Science.gov (United States)

2016-09-01

is and it would require some “fine-tuning.” [w] Mr. Peart finally has a physical architecture, and feels confident that it will meet the desired ...test and evaluation in systems engineering, this thesis examines the concept that test and evaluation, based on its desired early involvement in the...the documented roles of test and evaluation in systems engineering, this thesis examines the concept that test and evaluation, based on its desired

A Text-Based Chat System Embodied with an Expressive Agent

Directory of Open Access Journals (Sweden)

Lamia Alam

2017-01-01

Full Text Available Life-like characters are playing vital role in social computing by making human-computer interaction more easy and spontaneous. Nowadays, use of these characters to interact in online virtual environment has gained immense popularity. In this paper, we proposed a framework for a text-based chat system embodied with a life-like virtual agent that aims at natural communication between the users. To achieve this kind of system, we developed an agent that performs some nonverbal communications such as generating facial expression and motions by analyzing the text messages of the users. More specifically, this agent is capable of generating facial expressions for six basic emotions such as happy, sad, fear, angry, surprise, and disgust along with two additional emotions, irony and determined. Then to make the interaction between the users more realistic and lively, we added motions such as eye blink and head movements. We measured our proposed system from different aspects and found the results satisfactory, which make us believe that this kind of system can play a significant role in making an interaction episode more natural, effective, and interesting. Experimental evaluation reveals that the proposed agent can display emotive expressions correctly 93% of the time by analyzing the users’ text input.

The Influence of Gene Expression Time Delays on Gierer–Meinhardt Pattern Formation Systems

KAUST Repository

Seirin Lee, S.

2010-03-23

There are numerous examples of morphogen gradients controlling long range signalling in developmental and cellular systems. The prospect of two such interacting morphogens instigating long range self-organisation in biological systems via a Turing bifurcation has been explored, postulated, or implicated in the context of numerous developmental processes. However, modelling investigations of cellular systems typically neglect the influence of gene expression on such dynamics, even though transcription and translation are observed to be important in morphogenetic systems. In particular, the influence of gene expression on a large class of Turing bifurcation models, namely those with pure kinetics such as the Gierer-Meinhardt system, is unexplored. Our investigations demonstrate that the behaviour of the Gierer-Meinhardt model profoundly changes on the inclusion of gene expression dynamics and is sensitive to the sub-cellular details of gene expression. Features such as concentration blow up, morphogen oscillations and radical sensitivities to the duration of gene expression are observed and, at best, severely restrict the possible parameter spaces for feasible biological behaviour. These results also indicate that the behaviour of Turing pattern formation systems on the inclusion of gene expression time delays may provide a means of distinguishing between possible forms of interaction kinetics. Finally, this study also emphasises that sub-cellular and gene expression dynamics should not be simply neglected in models of long range biological pattern formation via morphogens. © 2010 Society for Mathematical Biology.

Efficient production of Trastuzumab Fab antibody fragments in Brevibacillus choshinensis expression system.

Science.gov (United States)

Mizukami, Makoto; Onishi, Hiromasa; Hanagata, Hiroshi; Miyauchi, Akira; Ito, Yuji; Tokunaga, Hiroko; Ishibashi, Matsujiro; Arakawa, Tsutomu; Tokunaga, Masao

2018-10-01

The Brevibacillus expression system has been successfully employed for the efficient productions of a variety of recombinant proteins, including enzymes, cytokines, antigens and antibody fragments. Here, we succeeded in secretory expression of Trastuzumab Fab antibody fragments using B. choshinensis/BIC (Brevibacillus in vivocloning) expression system. In the fed-batch high-density cell culture, recombinant Trastuzumab Fab with amino-terminal His-tag (His-BcFab) was secreted at high level, 1.25 g/liter, and Fab without His-tag (BcFab) at ∼145 mg/L of culture supernatant. His-BcFab and BcFab were purified to homogeneity using combination of conventional column chromatographies with a yield of 10-13%. This BcFab preparation exhibited native structure and functions evaluated by enzyme-linked immunosorbent assay, surface plasmon resonance, circular dichroism measurements and size exclusion chromatography. To our knowledge, this is the highest production of Fab antibody fragments in gram-positive bacterial expression/secretion systems. Copyright © 2018 Elsevier Inc. All rights reserved.

21 CFR 862.3660 - Phenobarbital test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Phenobarbital test system. 862.3660 Section 862....3660 Phenobarbital test system. (a) Identification. A phenobarbitol test system is a device intended to measure phenobarbital, an antiepileptic and sedative-hypnotic drug, in human specimens. Measurements...

Acceptance test procedure, 241-SY-101 Flexible Receiver System, Phase III testing

International Nuclear Information System (INIS)

Ritter, G.A.

1994-01-01

This Acceptance Test Procedure is for the 241-SY-101 Flexible Receiver System, Phase III Testing. This procedure will test the sealing integrity of the Flexible Receiver System to ensure that release of waste and aerosols will be minimized during the removal of the test mixer pump from tank SY-101

Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

Directory of Open Access Journals (Sweden)

Kronenwett Ralf

2012-10-01

Full Text Available Abstract Background EndoPredict (EP is a clinically validated multianalyte gene expression test to predict distant metastasis in ER-positive, HER2-negative breast cancer treated with endocrine therapy alone. The test is based on the combined analysis of 12 genes in formalin-fixed, paraffin-embedded (FFPE tissue by reverse transcription-quantitative real-time PCR (RT-qPCR. Recently, it was shown that EP is feasible for reliable decentralized assessment of gene expression. The aim of this study was the analytical validation of the performance characteristics of the assay and its verification in a molecular-pathological routine laboratory. Methods Gene expression values to calculate the EP score were assayed by one-step RT-qPCR using RNA from FFPE tumor tissue. Limit of blank, limit of detection, linear range, and PCR efficiency were assessed for each of the 12 PCR assays using serial samples dilutions. Different breast cancer samples were used to evaluate RNA input range, precision and inter-laboratory variability. Results PCR assays were linear up to Cq values between 35.1 and 37.2. Amplification efficiencies ranged from 75% to 101%. The RNA input range without considerable change of the EP score was between 0.16 and 18.5 ng/μl. Analysis of precision (variation of day, day time, instrument, operator, reagent lots resulted in a total noise (standard deviation of 0.16 EP score units on a scale from 0 to 15. The major part of the total noise (SD 0.14 was caused by the replicate-to-replicate noise of the PCR assays (repeatability and was not associated with different operating conditions (reproducibility. Performance characteristics established in the manufacturer’s laboratory were verified in a routine molecular pathology laboratory. Comparison of 10 tumor samples analyzed in two different laboratories showed a Pearson coefficient of 0.995 and a mean deviation of 0.15 score units. Conclusions The EP test showed reproducible performance

Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

International Nuclear Information System (INIS)

Kronenwett, Ralf; Brase, Jan C; Weber, Karsten E; Fisch, Karin; Müller, Berit M; Schmidt, Marcus; Filipits, Martin; Dubsky, Peter; Petry, Christoph; Dietel, Manfred; Denkert, Carsten; Bohmann, Kerstin; Prinzler, Judith; Sinn, Bruno V; Haufe, Franziska; Roth, Claudia; Averdick, Manuela; Ropers, Tanja; Windbergs, Claudia

2012-01-01

EndoPredict (EP) is a clinically validated multianalyte gene expression test to predict distant metastasis in ER-positive, HER2-negative breast cancer treated with endocrine therapy alone. The test is based on the combined analysis of 12 genes in formalin-fixed, paraffin-embedded (FFPE) tissue by reverse transcription-quantitative real-time PCR (RT-qPCR). Recently, it was shown that EP is feasible for reliable decentralized assessment of gene expression. The aim of this study was the analytical validation of the performance characteristics of the assay and its verification in a molecular-pathological routine laboratory. Gene expression values to calculate the EP score were assayed by one-step RT-qPCR using RNA from FFPE tumor tissue. Limit of blank, limit of detection, linear range, and PCR efficiency were assessed for each of the 12 PCR assays using serial samples dilutions. Different breast cancer samples were used to evaluate RNA input range, precision and inter-laboratory variability. PCR assays were linear up to C q values between 35.1 and 37.2. Amplification efficiencies ranged from 75% to 101%. The RNA input range without considerable change of the EP score was between 0.16 and 18.5 ng/μl. Analysis of precision (variation of day, day time, instrument, operator, reagent lots) resulted in a total noise (standard deviation) of 0.16 EP score units on a scale from 0 to 15. The major part of the total noise (SD 0.14) was caused by the replicate-to-replicate noise of the PCR assays (repeatability) and was not associated with different operating conditions (reproducibility). Performance characteristics established in the manufacturer’s laboratory were verified in a routine molecular pathology laboratory. Comparison of 10 tumor samples analyzed in two different laboratories showed a Pearson coefficient of 0.995 and a mean deviation of 0.15 score units. The EP test showed reproducible performance characteristics with good precision and negligible laboratory

Programmable, automated transistor test system

Science.gov (United States)

Truong, L. V.; Sundburg, G. R.

1986-01-01

A programmable, automated transistor test system was built to supply experimental data on new and advanced power semiconductors. The data will be used for analytical models and by engineers in designing space and aircraft electric power systems. A pulsed power technique was used at low duty cycles in a nondestructive test to examine the dynamic switching characteristic curves of power transistors in the 500 to 1000 V, 10 to 100 A range. Data collection, manipulation, storage, and output are operator interactive but are guided and controlled by the system software.

Fluoride exposure changed the structure and the expressions of Y chromosome related genes in testes of mice.

Science.gov (United States)

Cao, Jinling; Chen, Yan; Chen, Jianjie; Yan, Hanghang; Li, Meiyan; Wang, Jundong

2016-10-01

It is known that during spermatogenesis, pluripotent germ cells differentiate to become efficient delivery vehicles to the oocyte of paternal DNA, and the process is easily damaged by external poison. In this study, the effects of fluoride on the body weight, fluoride content in femur, testosterone levels in serum and testis, sperm quality, and the expressions of Y chromosome microdeletion genes and protein levels were examined in testes of Kunming male mice treated with different concentrations of 0, 25, 50, 100 mg/L of NaF in drinking water for 11 weeks, respectively. The results showed that compared with the control group, fluoride contents in three treatment groups were significantly increased and the structure of testes was seriously injured. The testosterone contents and the sperm count were decreased. Sperm malformation ratio was distinctly elevated. The expressions of Sly and HSF2 mRNA were markedly reduced in 100 mg/L NaF group and Ssty2 mRNA expression was dramatically decreased in 50 and 100 mg/L NaF groups. Meanwhile, the protein levels of Ssty2 and Sly were significantly reduced in 50 and 100 mg/L NaF groups and HSF2 protein levels were significantly decreased in 100 mg/L NaF group. These studies indicated that fluoride had toxic effects on male reproductive system by reducing the testosterone and sperm count, and increasing the sperm malformation ratio, supported by the damage of testicular structure, as a consequence of depressed HSF2 level, which resulted in the down-regulation of Ssty2 and Sly mRNA and protein. Copyright © 2016 Elsevier Ltd. All rights reserved.

Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

DEFF Research Database (Denmark)

Elias, Amer; Spector, Itay; Sogolovsky-Bard, Ilana

2016-01-01

Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a ...

Expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen.

Science.gov (United States)

Elgaied, Lamiaa; Salem, Reda; Elmenofy, Wael

2017-08-01

DNA encoding the coat protein (CP) of an Egyptian isolate of tomato yellow leaf curl virus (TYLCV) was inserted into the genome of Autographa californica nucleopolyhedrovirus (AcNPV) under the control of polyhedrin promoter. The generated recombinant baculovirus construct harboring the coat protein gene was characterized using PCR analysis. The recombinant coat protein expressed in infected insect cells was used as a coating antigen in an indirect Enzyme-linked immunosorbent assay (ELISA) and dot blot to test its utility for the detection of antibody generated against TYLCV virus particles. The results of ELISA and dot blot showed that the TYLCV-antibodies reacted positively with extracts of infected cells using the recombinant virus as a coating antigen with strong signals as well as the TYLCV infected tomato and beat plant extracts as positive samples. Scanning electron microscope examination showed that the expressed TYLCV coat protein was self-assembled into virus-like particles (VLPs) similar in size and morphology to TYLCV virus particles. These results concluded that, the expressed coat protein of TYLCV using baculovirus vector system is a reliable candidate for generation of anti-CP antibody for inexpensive detection of TYLCV-infected plants using indirect CP-ELISA or dot blot with high specificity.

Test-driven modeling of embedded systems

DEFF Research Database (Denmark)

Munck, Allan; Madsen, Jan

2015-01-01

To benefit maximally from model-based systems engineering (MBSE) trustworthy high quality models are required. From the software disciplines it is known that test-driven development (TDD) can significantly increase the quality of the products. Using a test-driven approach with MBSE may have...... a similar positive effect on the quality of the system models and the resulting products and may therefore be desirable. To define a test-driven model-based systems engineering (TD-MBSE) approach, we must define this approach for numerous sub disciplines such as modeling of requirements, use cases...... suggest that our method provides a sound foundation for rapid development of high quality system models....

Programmable automated transistor test system

International Nuclear Information System (INIS)

Truong, L.V.; Sundberg, G.R.

1986-01-01

The paper describes a programmable automated transistor test system (PATTS) and its utilization to evaluate bipolar transistors and Darlingtons, and such MOSFET and special types as can be accommodated with the PATTS base-drive. An application of a pulsed power technique at low duty cycles in a non-destructive test is used to examine the dynamic switching characteristic curves of power transistors. Data collection, manipulation, storage, and output are operator interactive but are guided and controlled by the system software. In addition a library of test data is established on disks, tapes, and hard copies for future reference

Availability of periodically tested systems

International Nuclear Information System (INIS)

Signoret, J.P.

1979-01-01

There is at the present time a need in accurate models to asess the availability of periodically tested stand-by systems. This paper shows how to improve the well known 'saw-tooth curve' model in order to take into account various reliability parameters. A model is developed to assess the pointwise and the mean availabilities of periodically tested stand-by systems. Exact and approxination formulae are given. In addition, the model developed herein leads to optimize the test interval in order to minimize the mean unavailability. A safety diesel in a nuclear power plant is given as an example

21 CFR 862.1225 - Creatinine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Creatinine test system. 862.1225 Section 862.1225....1225 Creatinine test system. (a) Identification. A creatinine test system is a device intended to measure creatinine levels in plasma and urine. Creatinine measurements are used in the diagnosis and...

21 CFR 862.3040 - Alcohol test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alcohol test system. 862.3040 Section 862.3040 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED....3040 Alcohol test system. (a) Identification. An alcohol test system is a device intented to measure...

21 CFR 862.1035 - Albumin test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Albumin test system. 862.1035 Section 862.1035....1035 Albumin test system. (a) Identification. An albumin test system is a device intended to measure the albumin concentration in serum and plasma. Albumin measurements are used in the diagnosis and...

Open Architecture Data System for NASA Langley Combined Loads Test System

Science.gov (United States)

Lightfoot, Michael C.; Ambur, Damodar R.

1998-01-01

The Combined Loads Test System (COLTS) is a new structures test complex that is being developed at NASA Langley Research Center (LaRC) to test large curved panels and cylindrical shell structures. These structural components are representative of aircraft fuselage sections of subsonic and supersonic transport aircraft and cryogenic tank structures of reusable launch vehicles. Test structures are subjected to combined loading conditions that simulate realistic flight load conditions. The facility consists of two pressure-box test machines and one combined loads test machine. Each test machine possesses a unique set of requirements or research data acquisition and real-time data display. Given the complex nature of the mechanical and thermal loads to be applied to the various research test articles, each data system has been designed with connectivity attributes that support both data acquisition and data management functions. This paper addresses the research driven data acquisition requirements for each test machine and demonstrates how an open architecture data system design not only meets those needs but provides robust data sharing between data systems including the various control systems which apply spectra of mechanical and thermal loading profiles.

21 CFR 862.1210 - Creatine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Creatine test system. 862.1210 Section 862.1210....1210 Creatine test system. (a) Identification. A creatine test system is a device intended to measure creatine (a substance synthesized in the liver and pancreas and found in biological fluids) in plasma...

21 CFR 862.1065 - Ammonia test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Ammonia test system. 862.1065 Section 862.1065....1065 Ammonia test system. (a) Identification. An ammonia test system is a device intended to measure ammonia levels in blood, serum, and plasma, Ammonia measurements are used in the diagnosis and treatment...

21 CFR 862.3600 - Mercury test system.

Science.gov (United States)

2010-04-01

....3600 Mercury test system. (a) Identification. A mercury test system is a device intended to measure mercury, a heavy metal, in human specimens. Measurements obtained by this device are used in the diagnosis... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Mercury test system. 862.3600 Section 862.3600...

Space Launch System Scale Model Acoustic Test Ignition Overpressure Testing

Science.gov (United States)

Nance, Donald; Liever, Peter; Nielsen, Tanner

2015-01-01

The overpressure phenomenon is a transient fluid dynamic event occurring during rocket propulsion system ignition. This phenomenon results from fluid compression of the accelerating plume gas, subsequent rarefaction, and subsequent propagation from the exhaust trench and duct holes. The high-amplitude unsteady fluid-dynamic perturbations can adversely affect the vehicle and surrounding structure. Commonly known as ignition overpressure (IOP), this is an important design-to environment for the Space Launch System (SLS) that NASA is currently developing. Subscale testing is useful in validating and verifying the IOP environment. This was one of the objectives of the Scale Model Acoustic Test, conducted at Marshall Space Flight Center. The test data quantifies the effectiveness of the SLS IOP suppression system and improves the analytical models used to predict the SLS IOP environments. The reduction and analysis of the data gathered during the SMAT IOP test series requires identification and characterization of multiple dynamic events and scaling of the event waveforms to provide the most accurate comparisons to determine the effectiveness of the IOP suppression systems. The identification and characterization of the overpressure events, the waveform scaling, the computation of the IOP suppression system knockdown factors, and preliminary comparisons to the analytical models are discussed.

Space Launch System Scale Model Acoustic Test Ignition Overpressure Testing

Science.gov (United States)

Nance, Donald K.; Liever, Peter A.

2015-01-01

The overpressure phenomenon is a transient fluid dynamic event occurring during rocket propulsion system ignition. This phenomenon results from fluid compression of the accelerating plume gas, subsequent rarefaction, and subsequent propagation from the exhaust trench and duct holes. The high-amplitude unsteady fluid-dynamic perturbations can adversely affect the vehicle and surrounding structure. Commonly known as ignition overpressure (IOP), this is an important design-to environment for the Space Launch System (SLS) that NASA is currently developing. Subscale testing is useful in validating and verifying the IOP environment. This was one of the objectives of the Scale Model Acoustic Test (SMAT), conducted at Marshall Space Flight Center (MSFC). The test data quantifies the effectiveness of the SLS IOP suppression system and improves the analytical models used to predict the SLS IOP environments. The reduction and analysis of the data gathered during the SMAT IOP test series requires identification and characterization of multiple dynamic events and scaling of the event waveforms to provide the most accurate comparisons to determine the effectiveness of the IOP suppression systems. The identification and characterization of the overpressure events, the waveform scaling, the computation of the IOP suppression system knockdown factors, and preliminary comparisons to the analytical models are discussed.

21 CFR 866.5040 - Albumin immunological test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Albumin immunological test system. 866.5040... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5040 Albumin immunological test system. (a) Identification. An albumin immunological test system is a device that consists of...

Tetracycline-inducible gene expression system in Leishmania mexicana

Czech Academy of Sciences Publication Activity Database

Kraeva, N.; Ishemgulova, A.; Lukeš, Julius; Yurchenko, Vyacheslav

2014-01-01

Roč. 198, č. 1 (2014), s. 11-13 ISSN 0166-6851 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Leishmania mexicana * Gene expression * Tet-inducible system Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.787, year: 2014

Frequency modulation system test procedure shuttle task 501 approach and landing test configuration

Science.gov (United States)

Doland, G. D.

1976-01-01

Shuttle Task 501 is an in-line task to test the performance and compatibility of radiofrequency links between the SSO and ground, and relay via a satellite. Under Shuttle Task 501 approach and landing test (ALT) phase only a limited portion of the communication and tracking (C&T) equipment is to be tested. The principal item to be tested is a frequency modulated (FM) data link. To test this RF link, an ALT FM System was designed, constructed, and the console wiring verified. A step-by-step procedure to be used to perform the ALT FM system is presented. The ALT FM system test is to be performed prior to delivery of the equipment to the Electronic Systems Test Laboratory (ESTL).

EXPRESS Rack Technology for Space Station

Science.gov (United States)

Davis, Ted B.; Adams, J. Brian; Fisher, Edward M., Jr.; Prickett, Guy B.; Smith, Timothy G.

1999-01-01

The EXPRESS rack provides accommodations for standard Mid-deck Locker and ISIS drawer payloads on the International Space Station. A design overview of the basic EXPRESS rack and two derivatives, the Human Research Facility and the Habitat Holding Rack, is given in Part I. In Part II, the design of the Solid State Power Control Module (SSPCM) is reviewed. The SSPCM is a programmable and remotely controllable power switching and voltage conversion unit which distributes and protects up to 3kW of 12OVDC and 28VDC power to payloads and rack subsystem components. Part III details the development and testing of a new data storage device, the BRP EXPRESS Memory Unit (BEMU). The BEMU is a conduction-cooled device which operates on 28VDC and is based on Boeing-modified 9GB commercial disk-drive technology. In Part IV results of a preliminary design effort for a rack Passive Damping System (PDS) are reported. The PDS is intended to isolate ISPR-based experiment racks from on-orbit vibration. System performance predictions based on component developmental testing indicate that such a system can provide effective isolation at frequencies of 1 Hz and above.

21 CFR 862.3110 - Antimony test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Antimony test system. 862.3110 Section 862.3110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3110 Antimony test system. (a) Identification. A...

A Physical Protection Systems Test Bed for International Counter-Trafficking System Development

International Nuclear Information System (INIS)

Stinson, Brad J.; Kuhn, Michael J.; Donaldson, Terrence L.; Richardson, Dave; Rowe, Nathan C.; Younkin, James R.; Pickett, Chris A.

2011-01-01

Physical protection systems have a widespread impact on the nuclear industry in areas such as nuclear safeguards, arms control, and trafficking of illicit goods (e.g., nuclear materials) across international borders around the world. Many challenges must be overcome in design and deployment of foreign border security systems such as lack of infrastructure, extreme environmental conditions, limited knowledge of terrain, insider threats, and occasional cultural resistance. Successful security systems, whether it be a system designed to secure a single facility or a whole border security system, rely on the entire integrated system composed of multiple subsystems. This test bed is composed of many unique sensors and subsystems, including wireless unattended ground sensors, a buried fiber-optic acoustic sensor, a lossy coaxial distributed sensor, wireless links, pan-tilt-zoom cameras, mobile power generation systems, unmanned aerial vehicles, and fiber-optic-fence intrusion detection systems. A Common Operating Picture software architecture is utilized to integrate a number of these subsystems. We are currently performance testing each system for border security and perimeter security applications by examining metrics such as probability of sense and a qualitative understanding of the sensors vulnerability of defeat. The testing process includes different soil conditions for buried sensors (e.g., dry, wet, and frozen) and an array of different tests including walking, running, stealth detection, and vehicle detection. Also, long term sustainability of systems is tested including performance differences due to seasonal variations (e.g. summer versus winter, while raining, in foggy conditions). The capabilities of the test bed are discussed. Performance testing results, both at the individual component level and integrated into a larger system for a specific deployment (in situ), help illustrate the usefulness and need for integrated testing facilities to carry out this

Cascade Distiller System Performance Testing Interim Results

Science.gov (United States)

Callahan, Michael R.; Pensinger, Stuart; Sargusingh, Miriam J.

2014-01-01

The Cascade Distillation System (CDS) is a rotary distillation system with potential for greater reliability and lower energy costs than existing distillation systems. Based upon the results of the 2009 distillation comparison test (DCT) and recommendations of the expert panel, the Advanced Exploration Systems (AES) Water Recovery Project (WRP) project advanced the technology by increasing reliability of the system through redesign of bearing assemblies and improved rotor dynamics. In addition, the project improved the CDS power efficiency by optimizing the thermoelectric heat pump (TeHP) and heat exchanger design. Testing at the NASA-JSC Advanced Exploration System Water Laboratory (AES Water Lab) using a prototype Cascade Distillation Subsystem (CDS) wastewater processor (Honeywell d International, Torrance, Calif.) with test support equipment and control system developed by Johnson Space Center was performed to evaluate performance of the system with the upgrades as compared to previous system performance. The system was challenged with Solution 1 from the NASA Exploration Life Support (ELS) distillation comparison testing performed in 2009. Solution 1 consisted of a mixed stream containing human-generated urine and humidity condensate. A secondary objective of this testing is to evaluate the performance of the CDS as compared to the state of the art Distillation Assembly (DA) used in the ISS Urine Processor Assembly (UPA). This was done by challenging the system with ISS analog waste streams. This paper details the results of the AES WRP CDS performance testing.

Theory of finite periodic systems - I: General expressions and various simple and illustrative examples

International Nuclear Information System (INIS)

Pereyra, Pedro; Castillo, Edith

2001-09-01

A comprehensive presentation of a new approach to finite periodic systems is given. The novel and general expressions obtained here, allow simple and precise calculations of various physical quantities characteristic of crystalline systems. Transmission amplitudes through n-cell multichannel quantum systems are rigorously derived. General expressions for several physical quantities are entirely expressed in terms of single-cell amplitudes and a new class of polynomials p N,n . Besides the general expressions, we study some superlattice properties as the band structure and its relation with the phase coherence phenomena, the level density and the Kronig-Penney model as its continuous espectrum limit. Bandstructure tailoring, optical multilayer systems, resonant energies and functions and channel-mixing effects in multichannel transport process are also analysed in the light of the new approach. (author)

Unit Testing for Command and Control Systems

Science.gov (United States)

Alexander, Joshua

2018-01-01

Unit tests were created to evaluate the functionality of a Data Generation and Publication tool for a command and control system. These unit tests are developed to constantly evaluate the tool and ensure it functions properly as the command and control system grows in size and scope. Unit tests are a crucial part of testing any software project and are especially instrumental in the development of a command and control system. They save resources, time and costs associated with testing, and catch issues before they become increasingly difficult and costly. The unit tests produced for the Data Generation and Publication tool to be used in a command and control system assure the users and stakeholders of its functionality and offer assurances which are vital in the launching of spacecraft safely.

GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives

Directory of Open Access Journals (Sweden)

Tim Kao

2016-09-01

Full Text Available The ability to reliably express fluorescent reporters or other genes of interest is important for using human pluripotent stem cells (hPSCs as a platform for investigating cell fates and gene function. We describe a simple expression system, designated GAPTrap (GT, in which reporter genes, including GFP, mCherry, mTagBFP2, luc2, Gluc, and lacZ are inserted into the GAPDH locus in hPSCs. Independent clones harboring variations of the GT vectors expressed remarkably consistent levels of the reporter gene. Differentiation experiments showed that reporter expression was reliably maintained in hematopoietic cells, cardiac mesoderm, definitive endoderm, and ventral midbrain dopaminergic neurons. Similarly, analysis of teratomas derived from GT-lacZ hPSCs showed that β-galactosidase expression was maintained in a spectrum of cell types representing derivatives of the three germ layers. Thus, the GAPTrap vectors represent a robust and straightforward tagging system that enables indelible labeling of PSCs and their differentiated derivatives.

Waste retrieval sluicing system data acquisition system acceptance test report

International Nuclear Information System (INIS)

Bevins, R.R.

1998-01-01

This document describes the test procedure for the Project W-320 Tank C-106 Sluicing Data Acquisition System (W-320 DAS). The Software Test portion will test items identified in the WRSS DAS System Description (SD), HNF-2115. Traceability to HNF-2115 will be via a reference that follows in parenthesis, after the test section title. The Field Test portion will test sensor operability, analog to digital conversion, and alarm setpoints for field instrumentation. The W-320 DAS supplies data to assist thermal modeling of tanks 241-C-106 and 241-AY-102. It is designed to be a central repository for information from sources that would otherwise have to be read, recorded, and integrated manually. Thus, completion of the DAS requires communication with several different data collection devices and output to a usable PC data formats. This test procedure will demonstrate that the DAS functions as required by the project requirements stated in Section 3 of the W-320 DAS System Description, HNF-2115

Evolution of a test article handling system for the SP-100 GES test

International Nuclear Information System (INIS)

Shen, E.J.; Schweiger, L.J.; Miller, W.C.; Gluck, R.; Davies, S.M.

1987-01-01

A simulated space environment test of a flight prototypic SP-100 reactor, control system, and flight shield will be conducted at the Hanford Engineering Development Laboratory (HEDL). The flight prototypic components and the supporting primary heat removal system are collectively known as the nuclear assembly test article (TA). The unique configuration and materials of fabrication for the Test Article require a specialized handling facility to support installation, maintenance, and final disposal operation. The test site operator, working in conjunction with the test article supplier, developed and evaluated several handling concepts resulting in the selection of a reference test article handling system. The development of the reference concept for the handling system is presented

The heterologous expression strategies of antimicrobial peptides in microbial systems.

Science.gov (United States)

Deng, Ting; Ge, Haoran; He, Huahua; Liu, Yao; Zhai, Chao; Feng, Liang; Yi, Li

2017-12-01

Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research. To conquer these obstacles, optimized heterologous expression technologies were developed that could provide effective ways to increase the yield of AMPs. In this review, the research progress on heterologous expression of AMPs using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Lightning vulnerability of nuclear explosive test systems at the Nevada Test Site

International Nuclear Information System (INIS)

Hasbrouck, R.T.

1985-01-01

A task force chartered to evaluate the effects of lightning on nuclear explosives at the Nevada Test Site has made several recommendations intended to provide lightning-invulnerable test device systems. When these recommendations have been implemented, the systems will be tested using full-threat-level simulated lightning

Cooperative Testing of Uncontrollable Timed Systems

DEFF Research Database (Denmark)

David, Alexandre; Larsen, Kim Guldstrand; Li, Shuhao

2008-01-01

the SUT against the test purpose as long as the SUT reacts to our moves in a cooperative style. We present an operational framework of cooperative winning strategy generation, test case derivation and execution. The test method is proved to be sound and complete. Preliminary experimental results indicate......Abstract. This paper deals with targeted testing of timed systems with uncontrollable behavior. The testing activity is viewed as a game between the tester and the system under test (SUT) towards a given test purpose. The SUT is modeled as Timed Game Automaton and the test purpose is specified...... in Timed CTL formula. We can employ a timed game solver UPPAAL-TIGA to check if the test purpose is ture w.r.t. the model, and if yes, to generate a winning strategy and use it for black-box conformance testing. Specifically, we show that in case the checking yields a negative result, we can still test...

Water electrolysis system refurbishment and testing

Science.gov (United States)

Greenough, B. M.

1972-01-01

The electrolytic oxygen generator for the back-up water electrolysis system in a 90-day manned test was refurbished, improved and subjected to a 182-day bench test. The performance of the system during the test demonstrated the soundness of the basic electrolysis concept, the high development status of the automatic controls which allowed completely hands-off operation, and the capability for orbital operation. Some design improvements are indicated.

Evolutionary tuning of protein expression levels of a positively autoregulated two-component system.

Directory of Open Access Journals (Sweden)

Rong Gao

2013-10-01

Full Text Available Cellular adaptation relies on the development of proper regulatory schemes for accurate control of gene expression levels in response to environmental cues. Over- or under-expression can lead to diminished cell fitness due to increased costs or insufficient benefits. Positive autoregulation is a common regulatory scheme that controls protein expression levels and gives rise to essential features in diverse signaling systems, yet its roles in cell fitness are less understood. It remains largely unknown how much protein expression is 'appropriate' for optimal cell fitness under specific extracellular conditions and how the dynamic environment shapes the regulatory scheme to reach appropriate expression levels. Here, we investigate the correlation of cell fitness and output response with protein expression levels of the E. coli PhoB/PhoR two-component system (TCS. In response to phosphate (Pi-depletion, the PhoB/PhoR system activates genes involved in phosphorus assimilation as well as genes encoding themselves, similarly to many other positively autoregulated TCSs. We developed a bacteria competition assay in continuous cultures and discovered that different Pi conditions have conflicting requirements of protein expression levels for optimal cell fitness. Pi-replete conditions favored cells with low levels of PhoB/PhoR while Pi-deplete conditions selected for cells with high levels of PhoB/PhoR. These two levels matched PhoB/PhoR concentrations achieved via positive autoregulation in wild-type cells under Pi-replete and -deplete conditions, respectively. The fitness optimum correlates with the wild-type expression level, above which the phosphorylation output saturates, thus further increase in expression presumably provides no additional benefits. Laboratory evolution experiments further indicate that cells with non-ideal protein levels can evolve toward the optimal levels with diverse mutational strategies. Our results suggest that the natural

[Role of CRISPR/Cas systems in drugresistance and virulence and the effect of IS600 on the expression of cse2 in Shigella].

Science.gov (United States)

Hong, Lijuan; Zhang, Bing; Duan, Guangcai; Liang, Wenjuan; Wang, Yingfang; Chen, Shuaiyin; Yang, Haiyan; Xi, Yuanlin

2016-12-04

To analyze the relationship between CRISPR/Cas system and drug-resistance, virulence. To investigate the effect of IS600 on the expression of CRISPR associated gene cse2 in Shigella. CRISPR loci, CRISPR associated gene cse2, drug-resistant genes and virulent genes were detected by PCR in 33 Shigella strains; Trypan Blue counting test was used to detect bacterial virulence; Real-time PCR was used to detect relative mRNA expression of cse2; susceptibilities of Shigella strains were tested by agar diffusion method. Furthermore, we analyzed the relationship between CRISPR loci and drug-resistant genes, virulent genes. The effect of the IS600 on the expression of CRISPR associated gene cse2 was investigated. The mortality of Hela cells infected by Shigella with CRISPR1 loci was significantly lower (PShigella without CRISPR1. The mRNA expression level of cse2 in group with IS600 was significantly (PShigella. Shigella without CRISPR1 has a higher pathogenicity. Due to the insertion of IS600, the mRNA expression level of cse2 was decreased in Shigella.

Farewell to the Earth and the Moon -ESA's Mars Express successfully tests its instruments

Science.gov (United States)

2003-07-01

The routine check-outs of Mars Express's instruments and of the Beagle-2 lander, performed during the last weeks, have been very successful. "As in all space missions little problems have arisen, but they have been carefully evaluated and solved. Mars Express continues on its way to Mars performing beautifully", comments Chicarro. The views of the Earth/Moon system were taken on 3 July 2003 by Mars Express's High Resolution Stereo Camera (HRSC), when the spacecraft was 8 million kilometres from Earth. The image taken shows true colours; the Pacific Ocean appears in blue, and the clouds near the Equator and in mid to northern latitudes in white to light grey. The image was processed by the Instrument Team at the Institute of Planetary Research of DLR, Berlin (Germany). It was built by combining a super resolution black and white HRSC snap-shot image of the Earth and the Moon with colour information obtained by the blue, green, and red sensors of the instrument. “The pictures and the information provided by the data prove the camera is working very well. They provide a good indication of what to expect once the spacecraft is in its orbit around Mars, at altitudes of only 250-300 kilometres: very high resolution images with brilliant true colour and in 3D,” says the Principal Investigator of the HRSC, Gerhard Neukum, of the Freie Universität of Berlin (Germany). This camera will be able to distinguish details of up to 2 metres on the Martian surface. Another striking demonstration of Mars Express's instruments high performance are the data taken by the OMEGA spectrometer. Once at Mars, this instrument will provide the best map of the molecular and mineralogical composition of the whole planet, with 5% of the planetary surface in high resolution. Minerals and other compounds such as water will be charted as never before. As the Red Planet is still too far away, the OMEGA team devised an ingenious test for their instrument: to detect the Earth’s surface

An introduction to testing techniques in the Intelsat TDMA/DSI system. II - Satellite system operations guide (SSOG) tests

Science.gov (United States)

Colby, R. J.; Parthasarathy, R.; Stimson, A. L.

1983-12-01

The test methods and procedures of the Intelsat TDMA/DSI SSOG are summarized. The overall structure of the SSOG is outlined, and the operational procedures to be followed for joining a new terminal to the system and for normal operations are reviewed, with an emphasis on the roles of the IOC and the TDMA reference and monitoring stations. The testing philosophy (based on minimal interruptions) and the star-test method are explained and illustrated with diagrams, and the test procedures are examined, including modem tests, electrical-path-length equalization, RF/IF downchain tests, protocol tests, nontransmitting protocol tests, IF/RF upchain tests, transmitting protocol tests, baseband tests, and orderwire lineups. The fundamental access discipline of the TDMA system is presented in an appendix.

Differential gene expressions in testes of L2 strain Taiwan country chicken in response to acute heat stress.

Science.gov (United States)

Wang, Shih-Han; Cheng, Chuen-Yu; Tang, Pin-Chi; Chen, Chih-Feng; Chen, Hsin-Hsin; Lee, Yen-Pai; Huang, San-Yuan

2013-01-15

Acute heat stress affects genes involved in spermatogenesis in mammals. However, there is apparently no elaborate research on the effects of acute heat stress on gene expression in avian testes. The purpose of this study was to investigate global gene expression in testes of the L2 strain of Taiwan country chicken after acute heat stress. Twelve roosters, 45 weeks old, were allocated into four groups, including control roosters kept at 25 °C, roosters subjected to 38 °C acute heat stress for 4 hours without recovery, with 2-hour recovery, and with 6-hour recovery, respectively. Testis samples were collected for RNA isolation and microarray analysis. Based on gene expression profiles, 169 genes were upregulated and 140 genes were downregulated after heat stress using a cutoff value of twofold or greater change. Based on gene ontology analysis, differentially expressed genes were mainly related to response to stress, transport, signal transduction, and metabolism. A functional network analysis displayed that heat shock protein genes and related chaperones were the major upregulated groups in chicken testes after acute heat stress. A quantitative real-time polymerase chain reaction analysis of mRNA expressions of HSP70, HSP90AA1, BAG3, SERPINB2, HSP25, DNAJA4, CYP3A80, CIRBP, and TAGLN confirmed the results of the microarray analysis. Because the HSP genes (HSP25, HSP70, and HSP90AA1) and the antiapoptotic BAG3 gene were dramatically altered in heat-stressed chicken testes, we concluded that these genes were important factors in the avian testes under acute heat stress. Whether these genes could be candidate genes for thermotolerance in roosters requires further investigation. Copyright © 2013 Elsevier Inc. All rights reserved.

Formulation and Analysis of an Approximate Expression for Voltage Sensitivity in Radial DC Distribution Systems

Directory of Open Access Journals (Sweden)

Ho-Yong Jeong

2015-08-01

Full Text Available Voltage is an important variable that reflects system conditions in DC distribution systems and affects many characteristics of a system. In a DC distribution system, there is a close relationship between the real power and the voltage magnitude, and this is one of major differences from the characteristics of AC distribution systems. One such relationship is expressed as the voltage sensitivity, and an understanding of voltage sensitivity is very useful to describe DC distribution systems. In this paper, a formulation for a novel approximate expression for the voltage sensitivity in a radial DC distribution system is presented. The approximate expression is derived from the power flow equation with some additional assumptions. The results of approximate expression is compared with an exact calculation, and relations between the voltage sensitivity and electrical quantities are analyzed analytically using both the exact form and the approximate voltage sensitivity equation.

Analysis of a Plant Transcriptional Regulatory Network Using Transient Expression Systems.

Science.gov (United States)

Díaz-Triviño, Sara; Long, Yuchen; Scheres, Ben; Blilou, Ikram

2017-01-01

In plant biology, transient expression systems have become valuable approaches used routinely to rapidly study protein expression, subcellular localization, protein-protein interactions, and transcriptional activity prior to in vivo studies. When studying transcriptional regulation, luciferase reporter assays offer a sensitive readout for assaying promoter behavior in response to different regulators or environmental contexts and to confirm and assess the functional relevance of predicted binding sites in target promoters. This chapter aims to provide detailed methods for using luciferase reporter system as a rapid, efficient, and versatile assay to analyze transcriptional regulation of target genes by transcriptional regulators. We describe a series of optimized transient expression systems consisting of Arabidopsis thaliana protoplasts, infiltrated Nicotiana benthamiana leaves, and human HeLa cells to study the transcriptional regulations of two well-characterized transcriptional regulators SCARECROW (SCR) and SHORT-ROOT (SHR) on one of their targets, CYCLIN D6 (CYCD6).Here, we illustrate similarities and differences in outcomes when using different systems. The plant-based systems revealed that the SCR-SHR complex enhances CYCD6 transcription, while analysis in HeLa cells showed that the complex is not sufficient to strongly induce CYCD6 transcription, suggesting that additional, plant-specific regulators are required for full activation. These results highlight the importance of the system and suggest that including heterologous systems, such as HeLa cells, can provide a more comprehensive analysis of a complex gene regulatory network.

PFN tool test and calibration system

International Nuclear Information System (INIS)

Stephenson, W.A.

1981-12-01

A system has been developed for the functional testing and neutron output calibration of the PFN (Prompt Fission Neutron) Uranium Logging Tool. The system was designed primarily for field work and consists of a special vehicle as well as test apparatus. Only the pertinent instrumentation is described. This document will serve as an Instruction and Test Equipment service manual for those involved with calibration of the neutron output of the PFN tool

Automated System Tests High-Power MOSFET's

Science.gov (United States)

Huston, Steven W.; Wendt, Isabel O.

1994-01-01

Computer-controlled system tests metal-oxide/semiconductor field-effect transistors (MOSFET's) at high voltages and currents. Measures seven parameters characterizing performance of MOSFET, with view toward obtaining early indication MOSFET defective. Use of test system prior to installation of power MOSFET in high-power circuit saves time and money.

Candidate innate immune system gene expression in the ecological model Daphnia.

Science.gov (United States)

Decaestecker, Ellen; Labbé, Pierrick; Ellegaard, Kirsten; Allen, Judith E; Little, Tom J

2011-10-01

The last ten years have witnessed increasing interest in host-pathogen interactions involving invertebrate hosts. The invertebrate innate immune system is now relatively well characterised, but in a limited range of genetic model organisms and under a limited number of conditions. Immune systems have been little studied under real-world scenarios of environmental variation and parasitism. Thus, we have investigated expression of candidate innate immune system genes in the water flea Daphnia, a model organism for ecological genetics, and whose capacity for clonal reproduction facilitates an exceptionally rigorous control of exposure dose or the study of responses at many time points. A unique characteristic of the particular Daphnia clones and pathogen strain combinations used presently is that they have been shown to be involved in specific host-pathogen coevolutionary interactions in the wild. We choose five genes, which are strong candidates to be involved in Daphnia-pathogen interactions, given that they have been shown to code for immune effectors in related organisms. Differential expression of these genes was quantified by qRT-PCR following exposure to the bacterial pathogen Pasteuria ramosa. Constitutive expression levels differed between host genotypes, and some genes appeared to show correlated expression. However, none of the genes appeared to show a major modification of expression level in response to Pasteuria exposure. By applying knowledge from related genetic model organisms (e.g. Drosophila) to models for the study of evolutionary ecology and coevolution (i.e. Daphnia), the candidate gene approach is temptingly efficient. However, our results show that detection of only weak patterns is likely if one chooses target genes for study based on previously identified genome sequences by comparison to homologues from other related organisms. Future work on the Daphnia-Pasteuria system will need to balance a candidate gene approach with more comprehensive

Test Confessions : A Study of Testing Practices for Plug-in Systems

NARCIS (Netherlands)

Greiler, M.; Van Deursen, A.; Storey, M.A.

2011-01-01

Testing plug-in-based systems is challenging due to complex interactions among many different plug-ins, and variations in version and configuration. The objective of this paper is to increase our understanding of what testers and developers think and do when it comes to testing plug-inbased systems.

Orion Ammonia Boiler System Preflight Test Preparations

Science.gov (United States)

Levitt, Julia L.

2017-01-01

The Environmental Controls and Life Support Systems (ECLSS) branch at Kennedy Space Center (KSC) is currently undergoing preparations for ground testing of the Orion Multi-Purpose Crew Vehicle (MPCV) to prepare its subsystems for EM-1 (Exploration Mission-1). EM-1, Orions second unmanned flight, is a three-week long lunar mission during which the vehicle will complete a 6-day retrograde lunar orbit before returning to Earth. This paper focuses on the work done during the authors 16-week internship with the Mechanical Engineering Branch of KSCs Engineering Directorate. The authors project involved assisting with the preparations for testing the Orion MPCVs ammonia boiler system. The purpose of the ammonia boiler system is to keep the spacecraft sufficiently cool during the reentry portion of its mission, from service module (SM) separation to post-landing. This system is critical for keeping both the spacecraft (avionics and electronics) and crew alive during reentry, thus a successful test of the system is essential to the success of EM-1. XXXX The author was able to draft a detailed outline of the procedure for the ammonia system functional test. More work will need to be done on the vehicle power-up and power-down portions of the procedure, but the ammonia system testing portion of the procedure is thorough and includes vehicle test configurations, vehicle commands, and GSE. The author was able to compile a substantial list of questions regarding the ammonia system functional test with the help of her mentors. A significant number of these questions were answered in the teleconferences with Lockheed Martin.

Testing of nuclear air-cleaning systems

International Nuclear Information System (INIS)

Anon.

1975-01-01

A standard is presented which describes methods for field-testing nuclear power plant air cleaning systems. Included are specifications for visual inspection; duct and housing leak test; mounting frame pressure leak test; airflow capacity, distribution, and residence time tests; air-aerosol mixing uniformity test; in place leak test of HEPA filter banks; multiple sampling technique; in-place leak test of adsorber stage; laboratory testing of adsorbent; and duct heater performance test

Test and control computer user's guide for a digital beam former test system

Science.gov (United States)

Alexovich, Robert E.; Mallasch, Paul G.

1992-01-01

A Digital Beam Former Test System was developed to determine the effects of noise, interferers and distortions, and digital implementations of beam forming as applied to the Tracking and Data Relay Satellite 2 (TDRS 2) architectures. The investigation of digital beam forming with application to TDRS 2 architectures, as described in TDRS 2 advanced concept design studies, was conducted by the NASA/Lewis Research Center for NASA/Goddard Space Flight Center. A Test and Control Computer (TCC) was used as the main controlling element of the digital Beam Former Test System. The Test and Control Computer User's Guide for a Digital Beam Former Test System provides an organized description of the Digital Beam Former Test System commands. It is written for users who wish to conduct tests of the Digital Beam forming Test processor using the TCC. The document describes the function, use, and syntax of the TCC commands available to the user while summarizing and demonstrating the use of the commands wtihin DOS batch files.

Preoperational test report, recirculation ventilation systems

Energy Technology Data Exchange (ETDEWEB)

Clifton, F.T.

1997-11-11

This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

Preoperational test report, recirculation ventilation systems

International Nuclear Information System (INIS)

Clifton, F.T.

1997-01-01

This represents a preoperational test report for Recirculation Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides vapor space cooling of tanks AY1O1, AY102, AZ1O1, AZ102 and supports the ability to exhaust air from each tank. Each system consists of a valved piping loop, a fan, condenser, and moisture separator; equipment is located inside each respective tank farm in its own hardened building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

21 CFR 862.1710 - Total triiodothyronine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Total triiodothyronine test system. 862.1710... Systems § 862.1710 Total triiodothyronine test system. (a) Identification. A total triiodothyronine test system is a device intended to measure the hormone triiodothyronine in serum and plasma. Measurements...

Spaceport Command and Control System Automation Testing

Science.gov (United States)

Hwang, Andrew

2017-01-01

The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administrations (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This large system requires high quality testing that will properly measure the capabilities of the system. Automating the test procedures would save the project time and money. Therefore, the Electrical Engineering Division at Kennedy Space Center (KSC) has recruited interns for the past two years to work alongside full-time engineers to develop these automated tests, as well as innovate upon the current automation process.

Spaceport Command and Control System Automated Testing

Science.gov (United States)

Stein, Meriel

2017-01-01

The Spaceport Command and Control System (SCCS) is the National Aeronautics and Space Administrations (NASA) launch control system for the Orion capsule and Space Launch System, the next generation manned rocket currently in development. This large system requires high quality testing that will properly measure the capabilities of the system. Automating the test procedures would save the project time and money. Therefore, the Electrical Engineering Division at Kennedy Space Center (KSC) has recruited interns for the past two years to work alongside full-time engineers to develop these automated tests, as well as innovate upon the current automation process.

Automatic test system of the photomultipliers

International Nuclear Information System (INIS)

Shiino, Kazuo; Kono, Koji; Ishii, Takanobu; Kasai, Seiji; Yamada, Sakue; Kitamura, Shoichi.

1990-03-01

A test system of R580 photomultipliers (PMTs) was constructed for the ZEUS experiment HERA. In this report, we will describe the general feature of the test system, each component of the setup, the procedure of the measurements, the data analyses and the results of the first 800 PMT measurements. (author)

Performance test results of mock-up test facility of HTTR hydrogen production system

International Nuclear Information System (INIS)

Ohashi, Hirofumi; Inaba, Yoshitomo; Nishihara, Tetsuo

2004-01-01

For the purpose to demonstrate effectiveness of high-temperature nuclear heat utilization, Japan Atomic Energy Research Institute has been developing a hydrogen production system and has planned to connect the hydrogen production system to High Temperature Engineering Test Reactor (HTTR). Prior to construction of a HTTR hydrogen production system, a mock-up test facility was constructed to investigate transient behavior of the hydrogen production system and to establish system controllability. The Mock-up test facility with a full-scale reaction tube is an approximately 1/30-scale model of the HTTR hydrogen production system and an electric heater is used as a heat source instead of a reactor. After its construction, a performance test of the test facility was carried out in the same pressure and temperature conditions as those of the HTTR hydrogen production system to investigate its performance such as hydrogen production ability, controllability and so on. It was confirmed that hydrogen was stably produced with a hot helium gas about 120m 3 /h, which satisfy the design value, and thermal disturbance of helium gas during the start-up could be mitigated within the design value by using a steam generator. The mock-up test of the HTTR hydrogen production system using this facility will continue until 2004. (author)

Testing cooperative systems with the MARS simulator

NARCIS (Netherlands)

Netten, B.D.; Wedemeijer, H.

2010-01-01

The complexity of cooperative systems makes the use of high fidelity simulation essential in the development and testing of cooperative applications and their interactions with other cooperative systems. In SAFESPOT a simulator test bench is setup to test the safety margin applications running on

ELECTROFORCE 3330 TEST SYSTEM

Data.gov (United States)

Federal Laboratory Consortium — The Bose Electroforce 3330 is a test system with an axial electromagnetic linear motor, a torsional motor, and an environmental chamber for high and low temperature...

Model-based testing for embedded systems

CERN Document Server

Zander, Justyna; Mosterman, Pieter J

2011-01-01

What the experts have to say about Model-Based Testing for Embedded Systems: "This book is exactly what is needed at the exact right time in this fast-growing area. From its beginnings over 10 years ago of deriving tests from UML statecharts, model-based testing has matured into a topic with both breadth and depth. Testing embedded systems is a natural application of MBT, and this book hits the nail exactly on the head. Numerous topics are presented clearly, thoroughly, and concisely in this cutting-edge book. The authors are world-class leading experts in this area and teach us well-used

21 CFR 862.1670 - Sorbitol dehydrogenase test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sorbitol dehydrogenase test system. 862.1670... Systems § 862.1670 Sorbitol dehydrogenase test system. (a) Identification. A sorbitol dehydrogenase test system is a device intended to measure the activity of the enzyme sorbitol dehydrogenase in serum...

21 CFR 862.1630 - Protein (fractionation) test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Protein (fractionation) test system. 862.1630... Systems § 862.1630 Protein (fractionation) test system. (a) Identification. A protein (fractionation) test system is a device intended to measure protein fractions in blood, urine, cerebrospinal fluid, and other...

Model-Driven Test Generation of Distributed Systems

Science.gov (United States)

Easwaran, Arvind; Hall, Brendan; Schweiker, Kevin

2012-01-01

This report describes a novel test generation technique for distributed systems. Utilizing formal models and formal verification tools, spe cifically the Symbolic Analysis Laboratory (SAL) tool-suite from SRI, we present techniques to generate concurrent test vectors for distrib uted systems. These are initially explored within an informal test validation context and later extended to achieve full MC/DC coverage of the TTEthernet protocol operating within a system-centric context.

The new pLAI (lux regulon based auto-inducible expression system for recombinant protein production in Escherichia coli

Directory of Open Access Journals (Sweden)

Nocadello Salvatore

2012-01-01

Full Text Available Abstract Background After many years of intensive research, it is generally assumed that no universal expression system can exist for high-level production of a given recombinant protein. Among the different expression systems, the inducible systems are the most popular for their tight regulation. However, induction is in many cases less favorable due to the high cost and/or toxicity of inducers, incompatibilities with industrial scale-up or detrimental growth conditions. Expression systems using autoinduction (or self-induction prove to be extremely versatile allowing growth and induction of recombinant proteins without the need to monitor cell density or add inducer. Unfortunately, almost all the actual auto inducible expression systems need endogenous or induced metabolic changes during the growth to trigger induction, both frequently linked to detrimental condition to cell growth. In this context, we use a simple modular approach for a cell density-based genetic regulation in order to assemble an autoinducible recombinant protein expression system in E. coli. Result The newly designed pLAI expression system places the expression of recombinant proteins in Escherichia coli under control of the regulatory genes of the lux regulon of Vibrio fischeri's Quorum Sensing (QS system. The pLAI system allows a tight regulation of the recombinant gene allowing a negligible basal expression and expression only at high cell density. Sequence optimization of regulative genes of QS of V. fischeri for expression in E. coli upgraded the system to high level expression. Moreover, partition of regulative genes between the plasmid and the host genome and introduction of a molecular safety lock permitted tighter control of gene expression. Conclusion Coupling gene expression to cell density using cell-to-cell communication provides a promising approach for recombinant protein production. The system allows the control of expression of the target recombinant gene

Construction of test-bed system of voltage management system to ...

African Journals Online (AJOL)

Construction of test-bed system of voltage management system to apply physical power system. ... Journal of Fundamental and Applied Sciences ... system of voltage management system (VMS) in order to apply physical power system.

Expression of CD64 on Circulating Neutrophils Favoring Systemic Inflammatory Status in Erythema Nodosum Leprosum.

Directory of Open Access Journals (Sweden)

Veronica Schmitz

2016-08-01

Full Text Available Erythema Nodosum Leprosum (ENL is an immune reaction in leprosy that aggravates the patient´s clinical condition. ENL presents systemic symptoms of an acute infectious syndrome with high leukocytosis and intense malaise clinically similar to sepsis. The treatment of ENL patients requires immunosuppression and thus needs to be early and efficient to prevent both disabilities and permanent nerve damage. Some patients experience multiple episodes of ENL and prolonged use of immunosuppressive drugs may lead to serious adverse effects. Thalidomide treatment is extremely effective at ameliorating ENL symptoms. Several mechanisms have been proposed to explain the efficacy of thalidomide in ENL, including the inhibition of TNF production. Given its teratogenicity, thalidomide is prohibitive for women of childbearing age. A rational search for molecular targets during ENL episodes is essential to better understand the disease mechanisms involved, which may also lead to the discovery of new drugs and diagnostic tests. Previous studies have demonstrated that IFN-γ and GM-CSF, involved in the induction of CD64 expression, increase during ENL. The aim of the present study was to investigate CD64 expression during ENL and whether thalidomide treatment modulated its expression. Leprosy patients were allocated to one of five groups: (1 Lepromatous leprosy, (2 Borderline leprosy, (3 Reversal reaction, (4 ENL, and (5 ENL 7 days after thalidomide treatment. The present study demonstrated that CD64 mRNA and protein were expressed in ENL lesions and that thalidomide treatment reduced CD64 expression and neutrophil infiltrates-a hallmark of ENL. We also showed that ENL blood neutrophils exclusively expressed CD64 on the cell surface and that thalidomide diminished overall expression. Patient classification based on clinical symptoms found that severe ENL presented high levels of neutrophil CD64. Collectively, these data revealed that ENL neutrophils express CD64

Preoperational test report, raw water system

Energy Technology Data Exchange (ETDEWEB)

Clifton, F.T.

1997-10-29

This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

Preoperational test report, raw water system

International Nuclear Information System (INIS)

Clifton, F.T.

1997-01-01

This represents the preoperational test report for the Raw Water System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system supplies makeup water to the W-030 recirculation evaporative cooling towers for tanks AY1O1, AY102, AZ1O1, AZ102. The Raw Water pipe riser and associated strainer and valving is located in the W-030 diesel generator building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

The Transrapid test facility between system development and system application

Energy Technology Data Exchange (ETDEWEB)

Baur, L [MVP GmbH, Muenchen (Germany)

1996-12-31

In the development of a new rail technology, such as the magnetic levitation, there is - in contrast to the further development of the railway technology - not the possibility to use existing routes for the technical verification of the system technology until the application. Instead of this there are two possibilities: Cost-effective preliminary development on test beds and small test facilities up to a development stage which justifies the (relatively serious) risk of realising a service route early and to conclude the system trials and verification there; cost-intensive construction of a large-scale test facility which permits an application-related verification of all important system functions and thus creates the technical pre-requisites for a low-risk system application; The presentation deals with the technical requirements of the system at the test facility the challenges and chances linked to its realisation and adjustment to the rapidly progressing state-of-the-art and which this way opens up for a minimisation of the technical application risk. (orig./HW)

Cyber-Physical Energy Systems Modeling, Test Specification, and Co-Simulation Based Testing

DEFF Research Database (Denmark)

van der Meer, A. A.; Palensky, P.; Heussen, Kai

2017-01-01

The gradual deployment of intelligent and coordinated devices in the electrical power system needs careful investigation of the interactions between the various domains involved. Especially due to the coupling between ICT and power systems a holistic approach for testing and validating is required....... Taking existing (quasi-) standardised smart grid system and test specification methods as a starting point, we are developing a holistic testing and validation approach that allows a very flexible way of assessing the system level aspects by various types of experiments (including virtual, real......, and mixed lab settings). This paper describes the formal holistic test case specification method and applies it to a particular co-simulation experimental setup. The various building blocks of such a simulation (i.e., FMI, mosaik, domain-specific simulation federates) are covered in more detail...

Equivalent Gene Expression Profiles between Glatopa™ and Copaxone®.

Directory of Open Access Journals (Sweden)

Josephine S D'Alessandro

Full Text Available Glatopa™ is a generic glatiramer acetate recently approved for the treatment of patients with relapsing forms of multiple sclerosis. Gene expression profiling was performed as a means to evaluate equivalence of Glatopa and Copaxone®. Microarray analysis containing 39,429 unique probes across the entire genome was performed in murine glatiramer acetate--responsive Th2-polarized T cells, a test system highly relevant to the biology of glatiramer acetate. A closely related but nonequivalent glatiramoid molecule was used as a control to establish assay sensitivity. Multiple probe-level (Student's t-test and sample-level (principal component analysis, multidimensional scaling, and hierarchical clustering statistical analyses were utilized to look for differences in gene expression induced by the test articles. The analyses were conducted across all genes measured, as well as across a subset of genes that were shown to be modulated by Copaxone. The following observations were made across multiple statistical analyses: the expression of numerous genes was significantly changed by treatment with Copaxone when compared against media-only control; gene expression profiles induced by Copaxone and Glatopa were not significantly different; and gene expression profiles induced by Copaxone and the nonequivalent glatiramoid were significantly different, underscoring the sensitivity of the test system and the multiple analysis methods. Comparative analysis was also performed on sets of transcripts relevant to T-cell biology and antigen presentation, among others that are known to be modulated by glatiramer acetate. No statistically significant differences were observed between Copaxone and Glatopa in the expression levels (magnitude and direction of these glatiramer acetate-regulated genes. In conclusion, multiple methods consistently supported equivalent gene expression profiles between Copaxone and Glatopa.

Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins.

Science.gov (United States)

Rozov, S M; Permyakova, N V; Deineko, E V

2018-03-01

Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O-glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.

Virtualization of System of Systems Test and Evaluation

Science.gov (United States)

2012-06-04

computers and is the primary enabler for virtualization. 2. Virtualization System Elements Parmalee, Peterson , Tillman, & Hatfield (1972) outlined the...The work of Abu-Taieh and El Sheikh, based on the work of Balci (1994, 1995), and Balci et al. ( 1996 ), seeks to organize types of tests and to...and testing. In A. Dasso & A. Funes (Eds.), Verification, validation, and testing in software engineering (pp. 155–184). Hershey , PA: Idea Group

An empirical likelihood ratio test robust to individual heterogeneity for differential expression analysis of RNA-seq.

Science.gov (United States)

Xu, Maoqi; Chen, Liang

2018-01-01

The individual sample heterogeneity is one of the biggest obstacles in biomarker identification for complex diseases such as cancers. Current statistical models to identify differentially expressed genes between disease and control groups often overlook the substantial human sample heterogeneity. Meanwhile, traditional nonparametric tests lose detailed data information and sacrifice the analysis power, although they are distribution free and robust to heterogeneity. Here, we propose an empirical likelihood ratio test with a mean-variance relationship constraint (ELTSeq) for the differential expression analysis of RNA sequencing (RNA-seq). As a distribution-free nonparametric model, ELTSeq handles individual heterogeneity by estimating an empirical probability for each observation without making any assumption about read-count distribution. It also incorporates a constraint for the read-count overdispersion, which is widely observed in RNA-seq data. ELTSeq demonstrates a significant improvement over existing methods such as edgeR, DESeq, t-tests, Wilcoxon tests and the classic empirical likelihood-ratio test when handling heterogeneous groups. It will significantly advance the transcriptomics studies of cancers and other complex disease. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Testing the psychometric properties of a Chinese version of the level of expressed emotion scale.

Science.gov (United States)

Chien, Wai Tong; Chan, Zenobia Chung-Yee; Chan, Sally Wai-Chi

2014-01-01

This study tested the psychometric properties of a Chinese version of the level of expressed emotion scale in Hong Kong Chinese patients with severe mental illness and their family caregivers. First, the semantic equivalence with the original English version and test-retest reliability at 2-week interval of the Chinese version was examined. After that, the reproducibility, construct validity, and internal consistency of the Chinese version were tested. The Chinese version indicated good semantic equivalence with the English version (kappa values = 0.76-0.95 and ICC = 0.81-0.92), test-retest reliability (r = 0.89-0.95, P Chinese version had substantial loadings on one of the four factors identified (intrusiveness/hostility, attitude towards patient, tolerance, and emotional involvement), accounting for 71.8% of the total variance of expressed emotion. In confirmatory factor analysis, the identified four-factor model showed the best fit based on all fit indices (χ (2)/df = 1.93, P = 0.75; AGFI = 0.96; TLI = 1.02; RMSEA = 0.031; WRMR = 0.78) to the collected data. The four-factor Chinese version also indicated a good concurrent validity with significant correlations with family functioning (r = -0.54) and family burden (r = 0.49) and a satisfactory reproducibility over six months (intraclass correlation coefficient of 0.90). The mean scores of the overall and subscale of the Chinese version in patients with unipolar disorder were higher than in other illness groups (schizophrenia, psychotic disorders, and bipolar disorder; P Chinese version demonstrates sound psychometric properties to measure families' expressed emotion in Chinese patients with severe mental illness, which are found varied across countries.

Sims Prototype System 2 test results: Engineering analysis

Science.gov (United States)

1978-01-01

The testing, problems encountered, and the results and conclusions obtained from tests performed on the IBM Prototype System, 2, solar hot water system, at the Marshall Space Flight Center Solar Test Facility was described. System 2 is a liquid, non draining solar energy system for supplying domestic hot water to single residences. The system consists of collectors, storage tank, heat exchanger, pumps and associated plumbing and controls.

Specification and Test of Real-Time Systems

DEFF Research Database (Denmark)

Nielsen, Brian

of the system, and a set of constraint patterns which describes and enforces the timing and synchronization constraints among components. We propose new techniques for automated black box conformance testing of real-time systems against densely timed speci cations. A test generator tool examines a specification......Distributed real-time computer based systems are very complex and intrinsically difficult to specify and implement correctly; in part this is caused by the overwhelming number of possible interactions between system components, but especially by a lack of adequate methods and tools to deal...... of the desired system behavior and generates the necessary test cases. A main problem is to construct a reasonably small test suite that can be executed within allotted resources, while having a high likelihood of detecting unknown errors. Our goal has been to treat the time dimension of this problem thoroughly...

Pain elicited by the Cold Pressor Test: A gender-comparative FACS coding study of spontaneous, faked and inhibited expressions.

OpenAIRE

Gil, Luisa; De Sousa, Cristina; Baunninger-Huber, Eva; Schiestl, Cathrin; Toussaint, Kyra; Gruber, Verena; Oliveira, Armando Monica; Duarte, Ana Catarina

2012-01-01

Evolutionary theories of pain have conjectured a better ability of males to control their facial expressions of pain, and of females to express and communicate emotions through the face. The present study involved 24 participants (12 men; 12 women). Pain was induced via the Cold Pressor Test (CPT), and three expressive contexts (spontaneous, faked an inhibited) were created through instructions. Elicited pain expressions were FACS coded and frequency, indices were derived for the observed Act...

A listening test system for automative audio

DEFF Research Database (Denmark)

Bech, Søren; Gulbol, Mehmet-Ali; Martin, Geoff

2005-01-01

This paper describes two listening tests that were performed to provide initial validation of an auralisation system (see Part 1) to mimic the acoustics of a car interior. The validation is based on a comparison of results from an in-car listening test and another test using the auralisation system...... and recordings of the stimuli used for the in-car test. The music samples for the test were chosen from a database of various CODEC examples from a previous extensive ITU test to validate the ITU-R BS.1387-1 standard....

Test procedure for boxed waste assay system

International Nuclear Information System (INIS)

Wachter, J.

1994-01-01

This document, prepared by Los Alamos National Laboratory's NMT-4 group, details the test methodology and requirements for Acceptance/Qualification testing of a Boxed Waste Assay System (BWAS) designed and constructed by Pajarito Scientific Corporation. Testing of the BWAS at the Plutonium Facility (TA55) at Los Alamos National Laboratory will be performed to ascertain system adherence to procurement specification requirements. The test program shall include demonstration of conveyor handling capabilities, gamma ray energy analysis, and imaging passive/active neutron accuracy and sensitivity. Integral to these functions is the system's embedded operating and data reduction software

System maintenance test plan for the TWRS controlled baseline database system

International Nuclear Information System (INIS)

Spencer, S.G.

1998-01-01

TWRS [Tank Waste Remediation System] Controlled Baseline Database, formally known as the Performance Measurement Control System, is used to track and monitor TWRS project management baseline information. This document contains the maintenance testing approach for software testing of the TCBD system once SCR/PRs are implemented

Facial expressions : What the mirror neuron system can and cannot tell us

NARCIS (Netherlands)

van der Gaag, Christiaan; Minderaa, Ruud B.; Keysers, Christian

2007-01-01

Facial expressions contain both motor and emotional components. The inferior frontal gyrus (IFG) and posterior parietal cortex have been considered to compose a mirror neuron system (MNS) for the motor components of facial expressions, while the amygdala and insula may represent an "additional" MNS

Multiloop integral system test (MIST)

International Nuclear Information System (INIS)

Gloudemans, J.R.

1989-07-01

The multiloop integral system test (MIST) was part of a multiphase program started in 1983 to address small-break loss-of-coolant accidents (SBLOCAs) specific to Babcock and Wilcox-designed plants. MIST was sponsored by the US Nuclear Regulatory Commission, the Babcock and Wilcox Owners Group, the Electric Power Research Institute, and Babcock and Wilcox. The unique features of the Babcock and Wilcox design, specifically the hot leg U-bends and steam generators, prevented the use of existing integral system data or existing integral system facilities to address the thermal-hydraulic SBLOCA questions. MIST and two other supporting facilities were specifically designed and constructed for this program, and an existing facility -- the once-through integral system (OTIS) -- was also used. Data from MIST and the other facilities will be used to benchmark the adequacy of system codes, such as RELAP5 and TRAC, for predicting abnormal plant transients. The individual tests are described in detail in Volumes 2 through 8 and Volume 11, and are summarized in Volume 1. Inter-group comparisons are addressed in this document, Volume 9. These comparisons are grouped as follows: mapping versus SBLOCA transients, SBLOCA, pump effects, and the effects of noncondensible gases. Appendix A provides an index and description of the microfiched plots for each test, which are enclosed with the corresponding Volumes 2 through 8. 147 figs., 5 tabs

Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems

Energy Technology Data Exchange (ETDEWEB)

Conover, David R. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Crawford, Alasdair J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Fuller, Jason [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Gourisetti, Sri Nikhil [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Viswanathan, Vilayanur [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ferreira, Summer Rhodes [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Schoenwald, David A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Rosewater, David Martin [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

2016-04-01

This Protocol provides a set of “best practices” for characterizing energy storage systems (ESSs) and measuring and reporting their performance. It serves as a basis for assessing how an ESS will perform with respect to key performance attributes relevant to different applications. It is intended to provide a valid and accurate basis for the comparison of different ESSs. By achieving the stated purpose, the Protocol will enable more informed decision-making in the selection of ESSs for various stationary applications. The Protocol identifies general information and technical specifications relevant in describing an ESS and also defines a set of test, measurement, and evaluation criteria with which to express the performance of ESSs that are intended for energy-intensive and/or power-intensive stationary applications. An ESS includes a storage device, battery management system, and any power conversion systems installed with the storage device. The Protocol is agnostic with respect to the storage technology and the size and rating of the ESS. The Protocol does not apply to single-use storage devices and storage devices that are not coupled with power conversion systems, nor does it address safety, security, or operations and maintenance of ESSs, or provide any pass/fail criteria.

40 CFR 792.43 - Test system care facilities.

Science.gov (United States)

2010-07-01

.... (a) A testing facility shall have a sufficient number of animal rooms or other test system areas, as... different tests. (b) A testing facility shall have a number of animal rooms or other test system areas... waste and refuse or for safe sanitary storage of waste before removal from the testing facility...

Preoperational test report, vent building ventilation system

International Nuclear Information System (INIS)

Clifton, F.T.

1997-01-01

This represents a preoperational test report for Vent Building Ventilation Systems, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides Heating, Ventilation, and Air Conditioning (HVAC) for the W-030 Ventilation Building. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System

Reconstructing dynamic mental models of facial expressions in prosopagnosia reveals distinct representations for identity and expression.

Science.gov (United States)

Richoz, Anne-Raphaëlle; Jack, Rachael E; Garrod, Oliver G B; Schyns, Philippe G; Caldara, Roberto

2015-04-01

The human face transmits a wealth of signals that readily provide crucial information for social interactions, such as facial identity and emotional expression. Yet, a fundamental question remains unresolved: does the face information for identity and emotional expression categorization tap into common or distinct representational systems? To address this question we tested PS, a pure case of acquired prosopagnosia with bilateral occipitotemporal lesions anatomically sparing the regions that are assumed to contribute to facial expression (de)coding (i.e., the amygdala, the insula and the posterior superior temporal sulcus--pSTS). We previously demonstrated that PS does not use information from the eye region to identify faces, but relies on the suboptimal mouth region. PS's abnormal information use for identity, coupled with her neural dissociation, provides a unique opportunity to probe the existence of a dichotomy in the face representational system. To reconstruct the mental models of the six basic facial expressions of emotion in PS and age-matched healthy observers, we used a novel reverse correlation technique tracking information use on dynamic faces. PS was comparable to controls, using all facial features to (de)code facial expressions with the exception of fear. PS's normal (de)coding of dynamic facial expressions suggests that the face system relies either on distinct representational systems for identity and expression, or dissociable cortical pathways to access them. Interestingly, PS showed a selective impairment for categorizing many static facial expressions, which could be accounted for by her lesion in the right inferior occipital gyrus. PS's advantage for dynamic facial expressions might instead relate to a functionally distinct and sufficient cortical pathway directly connecting the early visual cortex to the spared pSTS. Altogether, our data provide critical insights on the healthy and impaired face systems, question evidence of deficits

Orion Boiler Plate Airdrop Test System

Science.gov (United States)

Machin, Ricardo A.; Evans, Carol T.

2013-01-01

On the 29th of February 2012 the Orion Capsule Parachute Assembly System (CPAS) project attempted to perform an airdrop test of a boilerplate test article for the second time. The first attempt (Cluster Development Test 2, July 2008) to deliver a similar boilerplate from a C-17 using the Low Velocity Air Drop (LVAD) technique resulted in the programmer parachute failing to properly inflate, the test article failing to achieve the desired test initiation conditions, and the test article a total loss. This paper will pick up where the CDT-2 failure investigation left off, describing the test technique that was adopted, and outline the modeling that was performed to gain confidence that the second attempt would be successful. The second boiler plate test (Cluster Development Test 3-3) was indeed a complete success and has subsequently been repeated several times, allowing the CPAS project to proceed with the full scale system level development testing required to integrate the hardware to the first Entry Flight Test vehicle as well as go into the Critical Design Review with minimum risk and a mature design.

Tritium system test assembly control system cost estimate

International Nuclear Information System (INIS)

Stutz, R.A.

1979-01-01

The principal objectives of the Tritium Systems Test Assembly (TSTA), which includes the development, demonstration and interfacing of technologies related to the deuterium--tritium fuel cycle for fusion reactor systems, are concisely stated. The various integrated subsystems comprising TSTA and their functions are discussed. Each of the four major subdivisions of TSTA, including the main process system, the environmental and safety systems, supporting systems and the physical plant are briefly discussed. An overview of the Master Data Acquisition and Control System, which will control all functional operation of TSTA, is provided

Trigonellae Semen Enhances Sperm Motility and the Expression of the Cation Sperm Channel Proteins in Mouse Testes

Directory of Open Access Journals (Sweden)

Do Rim Kim

2015-01-01

Full Text Available Genetic defects during spermatogenesis can lead to a reduction in sperm motility and cause male infertility. The cation channels of sperm (CatSper play a role in the regulation of hyperactivated sperm motility in mouse testes. The effect of Trigonellae Semen (TS on the male reproductive system and CatSper protein in mouse testes during spermatogenesis was examined. C57BL/c mice were divided into the following five groups: normal, cyclophosphamide- (CP- only treated (control group, and three groups treated with varying concentrations of TS with CP (100, 500, and 1000 mg/kg TS and 100 mg/kg CP. Real-time PCR, western blot analysis, and a testosterone immunoassay were performed to assess CatSper protein levels in the five groups. Additionally, sperm cell counts and motility were examined. Results indicate that sperm motility and sperm counts increased in the TS treated groups in a dose-dependent manner (p<0.01. CatSper levels were also significantly higher in the TS treated groups compared to that of the control group (p<0.001. Therefore, TS treatment could enhance sperm function by promoting spermatogenesis and the expression of CatSper proteins in mouse testes.

Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.

Science.gov (United States)

Wang, Shih-Han; Cheng, Chuen-Yu; Chen, Chao-Jung; Chan, Hong-Lin; Chen, Hsin-Hsin; Tang, Pin-Chi; Chen, Chih-Feng; Lee, Yen-Pai; Huang, San-Yuan

2018-03-19

Heat stress leads to decreased fertility in roosters. This study investigated the global protein expression in response to acute heat stress in the testes of a broiler-type strain of Taiwan country chickens (TCCs). Twelve 45-week-old roosters were randomly allocated to the control group maintained at 25°C, and three groups subjected to acute heat stress at 38°C for 4 h, with 0, 2, and 6 h of recovery, respectively. Testis samples were collected for hematoxylin and eosin staining, apoptosis assay, and protein analysis. The results revealed 101 protein spots that differed significantly from the control following exposure to acute heat stress. The proteins that were differentially expressed participated mainly in protein metabolism and other metabolic processes, responses to stimuli, apoptosis, cellular organization, and spermatogenesis. Proteins that negatively regulate apoptosis were downregulated and proteins involved in autophagy and major heat shock proteins (HSP90α, HSPA5, and HSPA8) were upregulated in the testes of heat-stressed chickens. In conclusion, acute heat stress causes a change in protein expression in the testes of broiler-type B strain TCCs and may thus impair cell morphology, spermatogenesis, and apoptosis. The expression of heat shock proteins increased to attenuate the testicular injury induced by acute heat stress.

A prototype tap test imaging system: Initial field test results

Science.gov (United States)

Peters, J. J.; Barnard, D. J.; Hudelson, N. A.; Simpson, T. S.; Hsu, D. K.

2000-05-01

This paper describes a simple, field-worthy tap test imaging system that gives quantitative information about the size, shape, and severity of defects and damages. The system consists of an accelerometer, electronic circuits for conditioning the signal and measuring the impact duration, a laptop PC and data acquisition and processing software. The images are generated manually by tapping on a grid printed on a plastic sheet laid over the part's surface. A mechanized scanner is currently under development. The prototype has produced images for a variety of aircraft composite and metal honeycomb structures containing flaws, damages, and repairs. Images of the local contact stiffness, deduced from the impact duration using a spring model, revealed quantitatively the stiffness reduction due to flaws and damages, as well as the stiffness enhancement due to substructures. The system has been field tested on commercial and military aircraft as well as rotor blades and engine decks on helicopters. Field test results will be shown and the operation of the system will be demonstrated.—This material is based upon work supported by the Federal Aviation Administration under Contract #DTFA03-98-D-00008, Delivery Order No. IA016 and performed at Iowa State University's Center for NDE as part of the Center for Aviation Systems Reliability program.

Tutoring system for nondestructive testing using computer

Energy Technology Data Exchange (ETDEWEB)

Kim, Jin Koo; Koh, Sung Nam [Joong Ang Inspection Co.,Ltd., Seoul (Korea, Republic of); Shim, Yun Ju; Kim, Min Koo [Dept. of Computer Engineering, Aju University, Suwon (Korea, Republic of)

1997-10-15

This paper is written to introduce a multimedia tutoring system for nondestructive testing using personal computer. Nondestructive testing, one of the chief methods for inspecting welds and many other components, is very difficult for the NDT inspectors to understand its technical basis without a wide experience. And it is necessary for considerable repeated education and training for keeping their knowledge. The tutoring system that can simulate NDT works is suggested to solve the above problem based on reasonable condition. The tutoring system shows basic theories of nondestructive testing in a book-style with video images and hyper-links, and it offers practices, in which users can simulate the testing equipment. The book-style and simulation practices provide effective and individual environments for learning nondestructive testing.

Tutoring system for nondestructive testing using computer

International Nuclear Information System (INIS)

Kim, Jin Koo; Koh, Sung Nam; Shim, Yun Ju; Kim, Min Koo

1997-01-01

This paper is written to introduce a multimedia tutoring system for nondestructive testing using personal computer. Nondestructive testing, one of the chief methods for inspecting welds and many other components, is very difficult for the NDT inspectors to understand its technical basis without a wide experience. And it is necessary for considerable repeated education and training for keeping their knowledge. The tutoring system that can simulate NDT works is suggested to solve the above problem based on reasonable condition. The tutoring system shows basic theories of nondestructive testing in a book-style with video images and hyper-links, and it offers practices, in which users can simulate the testing equipment. The book-style and simulation practices provide effective and individual environments for learning nondestructive testing.

21 CFR 862.1715 - Triiodothyronine uptake test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Triiodothyronine uptake test system. 862.1715... Systems § 862.1715 Triiodothyronine uptake test system. (a) Identification. A triiodothyronine uptake test... plasma. Measurements of triiodothyronine uptake are used in the diagnosis and treatment of thyroid...

Timing of gene expression from different genetic systems in shaping ...

Indian Academy of Sciences (India)

2011-12-16

Dec 16, 2011 ... different genetic systems, nutrition quality traits were mainly controlled by the accumulative or net ... pable of providing valuable information on the expression of ...... protein, carbohydrates, and dietary fiber components.

Attributes of system testing which promote cost-effectiveness

International Nuclear Information System (INIS)

Martin, L.C.

1975-01-01

A brief overview of conventional EMP testing activity examines attributes of overall systems tests which promote cost-effectiveness. The general framework represents an EMP-oriented systems test as a portion of a planned program to design, produce, and field system elements. As such, all so-called system tests should play appropriate cost-effective roles in this program, and the objective here is to disclose such roles. The intrinsic worth of such tests depends not only upon placing proper values on the outcomes, but also upon the possible eventual consequences of not doing tests. A relative worth measure is required. Attributes of EMP system testing over the range of potential activity which encompasses research and development, production, field handling, verification, evaluation, and others are reviewed and examined. Thus, the relative worth, in a cost-effective sense, is provided by relating such attributes to the overall program objectives so that values can be placed on the outcomes for tradeoff purposes

Acute heat stress induces differential gene expressions in the testes of a broiler-type strain of Taiwan country chickens.

Science.gov (United States)

Wang, Shih-Han; Cheng, Chuen-Yu; Tang, Pin-Chi; Chen, Chih-Feng; Chen, Hsin-Hsin; Lee, Yen-Pai; Huang, San-Yuan

2015-01-01

The expression of testicular genes following acute heat stress has been reported in layer-type roosters, but few similar studies have been conducted on broilers. This study investigated the effect of acute heat stress on the gene expression in the testes of a broiler-type strain of Taiwan country chickens. Roosters were subjected to acute heat stress (38°C) for 4 h, and then exposed to 25°C, with testes collected 0, 2, and 6 h after the cessation of heat stress, using non-heat-stressed roosters as controls (n = 3 roosters per group). The body temperature and respiratory rate increased significantly (pstress. The numbers of apoptotic cells increased 2 h after the acute heat stress (79 ± 7 vs. 322 ± 192, control vs. heat stress; pstressed chickens from those of the controls, including genes involved in the response to stimulus, protein metabolism, signal transduction, cell adhesion, transcription, and apoptosis. The mRNA expressions of upregulated genes, including HSP25, HSP90AA1, HSPA2, and LPAR2, and of downregulated genes, including CDH5, CTNNA3, EHF, CIRBP, SLA, and NTF3, were confirmed through quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, numerous transcripts in the testes exhibited distinct expressions between the heat-stressed broiler-type and layer-type chickens. We concluded that the transcriptional responses of testes to acute heat stress may differ between the broiler-type and layer-type roosters. Whether the differential expression patterns associate with the heat-tolerance in the strains require a further exploration.

Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system

LENUS (Irish Health Repository)

Douillard, Francois P

2011-08-09

Abstract Background The development of the Nisin Inducible Controlled Expression (NICE) system in the food-grade bacterium Lactococcus lactis subsp. cremoris represents a cornerstone in the use of Gram-positive bacterial expression systems for biotechnological purposes. However, proteins that are subjected to such over-expression in L. lactis may suffer from improper folding, inclusion body formation and\\/or protein degradation, thereby significantly reducing the yield of soluble target protein. Although such drawbacks are not specific to L. lactis, no molecular tools have been developed to prevent or circumvent these recurrent problems of protein expression in L. lactis. Results Mimicking thioredoxin gene fusion systems available for E. coli, two nisin-inducible expression vectors were constructed to over-produce various proteins in L. lactis as thioredoxin fusion proteins. In this study, we demonstrate that our novel L. lactis fusion partner expression vectors allow high-level expression of soluble heterologous proteins Tuc2009 ORF40, Bbr_0140 and Tuc2009 BppU\\/BppL that were previously insoluble or not expressed using existing L. lactis expression vectors. Over-expressed proteins were subsequently purified by Ni-TED affinity chromatography. Intact heterologous proteins were detected by immunoblotting analyses. We also show that the thioredoxin moiety of the purified fusion protein was specifically and efficiently cleaved off by enterokinase treatment. Conclusions This study is the first description of a thioredoxin gene fusion expression system, purposely developed to circumvent problems associated with protein over-expression in L. lactis. It was shown to prevent protein insolubility and degradation, allowing sufficient production of soluble proteins for further structural and functional characterization.

21 CFR 862.3050 - Breath-alcohol test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Breath-alcohol test system. 862.3050 Section 862.3050 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED....3050 Breath-alcohol test system. (a) Identification. A breath-alcohol test system is a device intened...

Acceptance test report, 241-SY-101 Flexible Receiver System, Phase 3 testing

International Nuclear Information System (INIS)

Ritter, G.A.

1995-01-01

This document summarizes the results of the phase 3 acceptance test of the 241-SY-101 Flexible Receiver System (FRS). The purpose of this acceptance test is to verify the sealing integrity of the FRS to ensure that the release of waste and aerosols will be minimized during the removal of the test mixer pump from Tank 241-SY-101. The FRS is one of six major components of the Equipment Removal System, which has been designed to retrieve, transport, and store the mixer pump. This acceptance test was performed at the 306E Facility in the 300 area from January 10, 1995 to January 17, 1995. The Phase 3 test consisted of two parts. Part one was a water leak test of the seal between the blast shield and mock load distribution frame (LDF) to ensure that significant contamination of the pump pit and waste interaction with the aluminum impact-limiting material under the LDF are prevented during the pump removal operation. The second part of this acceptance test was an air leak test of the assembled flexible receiver system. The purpose of this test was to verify that the release of hazardous aerosols will be minimized if the tank dome pressure becomes slightly positive during the decontamination of the mixer pump

Automatic Test Systems Aquisition

National Research Council Canada - National Science Library

1994-01-01

We are providing this final memorandum report for your information and use. This report discusses the efforts to achieve commonality in standards among the Military Departments as part of the DoD policy for automatic test systems (ATS...

GES [Ground Engineering System] test site preparation

International Nuclear Information System (INIS)

Cox, C.M.; Mahaffey, M.K.; Miller, W.C.; Schade, A.R.; Toyoda, K.G.

1987-10-01

Activities are under way at Hanford to convert the 309 containment building and its associated service wing to a nuclear test facility for the Ground Engineering System (GES) test. Conceptual design is about 80% complete, encompassing facility modifications, a secondary heat transport system, a large vacuum system, a test article cell and handing system, control and data handling systems, and safety andl auxiliary systems. The design makes extensive use of existing equipment to minimize technical risk and cost. Refurbishment of this equipment is 25% complete. Cleanout of some 1000 m 3 of equipment from the earlier reactor test in the facility is 85% complete. An Environmental Assessment was prepared and revised to incorporate Department of Energy (DOE) comments. It is now in the DOE approval chain, where a Finding of No Significant Impact is expected. During the next year, definite design will be well advanced, long-lead procurements will be initiated, construction planning will be completed, an operator training plan will be prepared, and the site (preliminary) safety analysis report will be drafted

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.

Directory of Open Access Journals (Sweden)

Yoichiro Ito

Full Text Available Combinatorial screening used together with a broad library of gene expression cassettes is expected to produce a powerful tool for the optimization of the simultaneous expression of multiple enzymes. Recently, we proposed a highly tunable protein expression system that utilized multiple genome-integrated target genes to fine-tune enzyme expression in yeast cells. This tunable system included a library of expression cassettes each composed of three gene-expression control elements that in different combinations produced a wide range of protein expression levels. In this study, four gene expression cassettes with graded protein expression levels were applied to the expression of three cellulases: cellobiohydrolase 1, cellobiohydrolase 2, and endoglucanase 2. After combinatorial screening for transgenic yeasts simultaneously secreting these three cellulases, we obtained strains with higher cellulase expressions than a strain harboring three cellulase-expression constructs within one high-performance gene expression cassette. These results show that our method will be of broad use throughout the field of metabolic engineering.

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System

Science.gov (United States)

Ito, Yoichiro; Yamanishi, Mamoru; Ikeuchi, Akinori; Imamura, Chie; Matsuyama, Takashi

2015-01-01

Combinatorial screening used together with a broad library of gene expression cassettes is expected to produce a powerful tool for the optimization of the simultaneous expression of multiple enzymes. Recently, we proposed a highly tunable protein expression system that utilized multiple genome-integrated target genes to fine-tune enzyme expression in yeast cells. This tunable system included a library of expression cassettes each composed of three gene-expression control elements that in different combinations produced a wide range of protein expression levels. In this study, four gene expression cassettes with graded protein expression levels were applied to the expression of three cellulases: cellobiohydrolase 1, cellobiohydrolase 2, and endoglucanase 2. After combinatorial screening for transgenic yeasts simultaneously secreting these three cellulases, we obtained strains with higher cellulase expressions than a strain harboring three cellulase-expression constructs within one high-performance gene expression cassette. These results show that our method will be of broad use throughout the field of metabolic engineering. PMID:26692026

Testing Real-Time Systems Using UPPAAL

DEFF Research Database (Denmark)

Hessel, Anders; Larsen, Kim Guldstrand; Mikucionis, Marius

2008-01-01

This chapter presents principles and techniques for model-based black-box conformance testing of real-time systems using the Uppaal model-checking tool-suite. The basis for testing is given as a network of concurrent timed automata specified by the test engineer. Relativized input...

Beam test with the HIMAC RF control system

International Nuclear Information System (INIS)

Kanazawa, M.; Sato, K.; Itano, A.

1992-01-01

RF system of the HIMAC synchrotron has been developed and tested in the factory. With the high power system, we could sweep the acceleration frequency from 1MHz to 8MHz with the acceleration voltage of 6KV. The performance of the RF control system has been confirmed with a developed simulator of the synchrotron oscillation. Following these two tests in the factory, we had a beam test of the RF control system at TARN-II in INS (Institute for Nuclear Study, University of Tokyo). This paper describes the beam test and its results. (author)

Three-Step Test System for the Identification of Novel GABAA Receptor Modulating Food Plants.

Science.gov (United States)

Sahin, Sümeyye; Eulenburg, Volker; Kreis, Wolfgang; Villmann, Carmen; Pischetsrieder, Monika

2016-12-01

Potentiation of γ-amino butyric acid (GABA)-induced GABA A receptor (GABA A R) activation is a common pathway to achieve sedative, sleep-enhancing, anxiolytic, and antidepressant effects. Presently, a three-component test system was established for the identification of novel GABA A R modulating food plants. In the first step, potentiation of GABA-induced response of the GABA A R was analysed by two-electrode voltage clamp (TEVC) for activity on human α1β2-GABA A R expressed in Xenopus laevis oocytes. Positively tested food plants were then subjected to quantification of GABA content by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) to exclude test foods, which evoke a TEVC-response by endogenous GABA. In the third step, specificity of GABA A -modulating activity was assessed by TEVC analysis of Xenopus laevis oocytes expressing the homologous glycine receptor (GlyR). The three-component test was then applied to screen 10 aqueous extracts of food plants for their GABA A R activity. Thus, hop cones (Humulus lupulus) and Sideritis sipylea were identified as the most potent specific GABA A R modulators eliciting significant potentiation of the current by 182 ± 27 and 172 ± 19 %, respectively, at the lowest concentration of 0.5 μg/mL. The extracts can now be further evaluated by in vivo studies and by structural evaluation of the active components.

Stress Testing of Transactional Database Systems

OpenAIRE

Meira , Jorge Augusto; Cunha De Almeida , Eduardo; Sunyé , Gerson; Le Traon , Yves; Valduriez , Patrick

2013-01-01

International audience; Transactional database management systems (DBMS) have been successful at supporting traditional transaction processing workloads. However, web-based applications that tend to generate huge numbers of concurrent business operations are pushing DBMS performance over their limits, thus threatening overall system availability. Then, a crucial question is how to test DBMS performance under heavy workload conditions. Answering this question requires a testing methodology to ...

Development of autonomous vehicles’ testing system

Science.gov (United States)

Ivanov, A. M.; Shadrin, S. S.

2018-02-01

This article describes overview of automated and, in perspective, autonomous vehicles’ (AV) implementation risks. Set of activities, actual before the use of AVs on public roads, minimizing negative technical and social problems of AVs’ implementation is presented. Classification of vehicle’s automated control systems operating conditions is formulated. Groups of tests for AVs are developed and justified, sequence of AVs’ testing system formation is proposed.

Simulator testing system (STS)

International Nuclear Information System (INIS)

Miller, V.N.

1990-01-01

In recent years there has been a greater demand placed on the capabilities and time usage of real-time nuclear plant simulators due to NRC, INPO and utilities requirements. The requirements applied to certification, new simulators, upgrades, modifications, and maintenance of the simulators vary; however, they all require the capabilities of the simulator to be tested whether it is for NRC 10CFR55.45b requirements, ATP testing of new simulators, ATP testing of upgrades with or without panels, adding software/hardware due to plant modifications, or analyzing software/hardware problems on the simulator. This paper describes the Simulator Testing System (STS) which addresses each one of these requirements placed on simulators. Special attention will be given to ATP testing of upgrades without the use of control room panels. The capabilities and applications of the four parts of STS which are the Display Control Software (DCS), Procedure Control Software (PCS), Display Generator Software (DGS) and the Procedure Generator Software (PGS) will be reviewed

Radioactive waste incineration system cold demonstration test

Energy Technology Data Exchange (ETDEWEB)

Hozumi, Masahiro; Takaoku, Yoshinobu; Koyama, Shigeru; Nagae, Madoka; Seike, Yasuhiko; Yamanaka, Yasuhiro; Shibata, Kenji; Manabe, Kyoichi

1984-12-01

To demonstrate Waste Incineration System (WIS) which our company has been licensed by Combustion Engineering Inc., USA we installed a demonstration test plant in our Hiratsuka Research Laboratory and started the demonstration test on January 1984. One of the characteristics of this system is to be able to process many kinds of wastes with only one system, and to get high volume reduction factors. In our test plant, we processed paper, cloth, wood, polyethylene sheets as the samples of solid combustible wastes and spent ion exchange resins with incineration and processed condensed liquid wastes with spray drying. We have got good performances and enough Decontamination Factor (DF) data for the dust control equipment. In this paper, we introduce this demonstration test plant and report the test results up to date. (author).

Finding differentially expressed genes in high dimensional data: Rank based test statistic via a distance measure.

Science.gov (United States)

Mathur, Sunil; Sadana, Ajit

2015-12-01

We present a rank-based test statistic for the identification of differentially expressed genes using a distance measure. The proposed test statistic is highly robust against extreme values and does not assume the distribution of parent population. Simulation studies show that the proposed test is more powerful than some of the commonly used methods, such as paired t-test, Wilcoxon signed rank test, and significance analysis of microarray (SAM) under certain non-normal distributions. The asymptotic distribution of the test statistic, and the p-value function are discussed. The application of proposed method is shown using a real-life data set. © The Author(s) 2011.

21 CFR 864.9300 - Automated Coombs test systems.

Science.gov (United States)

2010-04-01

... Blood and Blood Products § 864.9300 Automated Coombs test systems. (a) Identification. An automated Coombs test system is a device used to detect and identify antibodies in patient sera or antibodies bound... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated Coombs test systems. 864.9300 Section...

21 CFR 862.1770 - Urea nitrogen test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Urea nitrogen test system. 862.1770 Section 862....1770 Urea nitrogen test system. (a) Identification. A urea nitrogen test system is a device intended to measure urea nitrogen (an end-product of nitrogen metabolism) in whole blood, serum, plasma, and urine...

Testing the Psychometric Properties of a Chinese Version of the Level of Expressed Emotion Scale

Directory of Open Access Journals (Sweden)

Wai Tong Chien

2014-01-01

Full Text Available This study tested the psychometric properties of a Chinese version of the level of expressed emotion scale in Hong Kong Chinese patients with severe mental illness and their family caregivers. First, the semantic equivalence with the original English version and test-retest reliability at 2-week interval of the Chinese version was examined. After that, the reproducibility, construct validity, and internal consistency of the Chinese version were tested. The Chinese version indicated good semantic equivalence with the English version (kappa values = 0.76–0.95 and ICC = 0.81–0.92, test-retest reliability (r = 0.89–0.95, P<0.01, and internal consistency (Cronbach’s α = 0.86–0.92. Among 262 patients with severe mental illness and their caregivers, the 50-item Chinese version had substantial loadings on one of the four factors identified (intrusiveness/hostility, attitude towards patient, tolerance, and emotional involvement, accounting for 71.8% of the total variance of expressed emotion. In confirmatory factor analysis, the identified four-factor model showed the best fit based on all fit indices (χ2/df = 1.93, P=0.75; AGFI = 0.96; TLI = 1.02; RMSEA = 0.031; WRMR = 0.78 to the collected data. The four-factor Chinese version also indicated a good concurrent validity with significant correlations with family functioning (r = −0.54 and family burden (r = 0.49 and a satisfactory reproducibility over six months (intraclass correlation coefficient of 0.90. The mean scores of the overall and subscale of the Chinese version in patients with unipolar disorder were higher than in other illness groups (schizophrenia, psychotic disorders, and bipolar disorder; P<0.01. The Chinese version demonstrates sound psychometric properties to measure families’ expressed emotion in Chinese patients with severe mental illness, which are found varied across countries.

The Method of System Test using I/O Stimulator

Energy Technology Data Exchange (ETDEWEB)

Kim, Jang Yeol; Lee, Dong Young; Kim, Chang Hoi [KAERI, Daejeon (Korea, Republic of)

2016-05-15

In general, the software development life cycle consists of the requirement phase, design phase, implementation phase, testing phase, integration phase (software-software integration, software-hardware integration), installation phase, and operation and maintenance phase. The fundamental parts to the testing phase are component test, software integration test, software hardware integration test, and system test. Among these tests, we focused in particular on system test using I/O stimulator. The system test operates the functionality, performance, and interface because system testing falls within the scope of black box testing, which should not require knowledge of the inner design of the code or logic. The purpose of developing a system test using a I/O stimulator is to save time and effort from the functionality and performance tests. We developed an I/O stimulator for the burning system test based on the operational scenario. The hardware, software, and manmachine- interface (MMI) are fundamental parts to the I/O stimulator. Signal sources from the I/O stimulator contain analog input, analog output, digital input, digital output, a Programmable Power Supply, an RS232C Serial interface and a dual port Ethernet interface. While automation cannot reproduce everything that a software engineer can do, it can be extremely useful for the system test. However, it does require the test criteria and a well-developed test suite of the testing scripts in order to be useful. In this study, we developed a multipurpose and cost-efficient I/O stimulator using LabVIEW program instead of the testing scripts.

The Method of System Test using I/O Stimulator

International Nuclear Information System (INIS)

Kim, Jang Yeol; Lee, Dong Young; Kim, Chang Hoi

2016-01-01

In general, the software development life cycle consists of the requirement phase, design phase, implementation phase, testing phase, integration phase (software-software integration, software-hardware integration), installation phase, and operation and maintenance phase. The fundamental parts to the testing phase are component test, software integration test, software hardware integration test, and system test. Among these tests, we focused in particular on system test using I/O stimulator. The system test operates the functionality, performance, and interface because system testing falls within the scope of black box testing, which should not require knowledge of the inner design of the code or logic. The purpose of developing a system test using a I/O stimulator is to save time and effort from the functionality and performance tests. We developed an I/O stimulator for the burning system test based on the operational scenario. The hardware, software, and manmachine- interface (MMI) are fundamental parts to the I/O stimulator. Signal sources from the I/O stimulator contain analog input, analog output, digital input, digital output, a Programmable Power Supply, an RS232C Serial interface and a dual port Ethernet interface. While automation cannot reproduce everything that a software engineer can do, it can be extremely useful for the system test. However, it does require the test criteria and a well-developed test suite of the testing scripts in order to be useful. In this study, we developed a multipurpose and cost-efficient I/O stimulator using LabVIEW program instead of the testing scripts

Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System

Directory of Open Access Journals (Sweden)

Mohammed N. Baeshen

2016-01-01

Full Text Available Increase in the incidence of Insulin Dependent Diabetes Mellitus (IDDM among people from developed and developing countries has created a large global market for insulin. Moreover, exploration of new methods for insulin delivery including oral or inhalation route which require very high doses would further increase the demand of cost-effective recombinant insulin. Various bacterial and yeast strains have been optimized to overproduce important biopharmaceuticals. One of the approaches we have taken is the production of recombinant human insulin along with C-peptide in yeast Pichia pastoris. We procured a cDNA clone of insulin from Origene Inc., USA. Insulin cDNA was PCR amplified and cloned into yeast vector pPICZ-α. Cloned insulin cDNA was confirmed by restriction analysis and DNA sequencing. pPICZ-α-insulin clone was transformed into Pichia pastoris SuperMan5 strain. Several Zeocin resistant clones were obtained and integration of insulin cDNA in Pichia genome was confirmed by PCR using insulin specific primers. Expression of insulin in Pichia clones was confirmed by ELISA, SDS-PAGE, and Western blot analysis. In vivo efficacy studies in streptozotocin induced diabetic mice confirmed the activity of recombinant insulin. In conclusion, a biologically active human proinsulin along with C-peptide was expressed at high level using Pichia pastoris expression system.

21 CFR 862.1815 - Vitamin E test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Vitamin E test system. 862.1815 Section 862.1815....1815 Vitamin E test system. (a) Identification. A vitamin E test system is a device intended to measure vitamin E (tocopherol) in serum. Measurements obtained by this device are used in the diagnosis and...

21 CFR 862.1450 - Lactic acid test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Lactic acid test system. 862.1450 Section 862.1450....1450 Lactic acid test system. (a) Identification. A lactic acid test system is a device intended to measure lactic acid in whole blood and plasma. Lactic acid measurements that evaluate the acid-base status...

21 CFR 862.1730 - Free tyrosine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Free tyrosine test system. 862.1730 Section 862....1730 Free tyrosine test system. (a) Identification. A free tyrosine test system is a device intended to measure free tyrosine (an amono acid) in serum and urine. Measurements obtained by this device are used in...

Prototype Engineered Barrier System Field Tests

International Nuclear Information System (INIS)

Ramirez, A.L.; Beatty, J.; Buscheck, T.A.

1989-01-01

This paper presents selected preliminary results obtained during the first 54 days of the Prototype Engineered Barrier System Field Tests (PEBSFT) that are being performed in G-Tunnel within the Nevada Test Site. The test described is a precursor to the Engineered Barrier Systems Field Tests (EBSFT). The EBSFT will consist of in situ tests of the geohydrologic and geochemical environment in the near field (within a few meters) of heaters emplaced in welded tuff to simulate the thermal effects of waste packages. The PEBSFTs are being conducted to evaluate the applicability of measurement techniques, numerical models, and procedures for future investigations that will be conducted in the Exploratory Shaft Facilities of the Yucca Mountain Project (YMP). The paper discusses the evolution of hydrothermal behavior during the prototype test, including rock temperatures, changes in rock moisture content, air permeability of fractures, gas pressures, and rock mass gas-phase humidity. 10 refs., 12 figs

TRENDS: The aeronautical post-test database management system

Science.gov (United States)

Bjorkman, W. S.; Bondi, M. J.

1990-01-01

TRENDS, an engineering-test database operating system developed by NASA to support rotorcraft flight tests, is described. Capabilities and characteristics of the system are presented, with examples of its use in recalling and analyzing rotorcraft flight-test data from a TRENDS database. The importance of system user-friendliness in gaining users' acceptance is stressed, as is the importance of integrating supporting narrative data with numerical data in engineering-test databases. Considerations relevant to the creation and maintenance of flight-test database are discussed and TRENDS' solutions to database management problems are described. Requirements, constraints, and other considerations which led to the system's configuration are discussed and some of the lessons learned during TRENDS' development are presented. Potential applications of TRENDS to a wide range of aeronautical and other engineering tests are identified.

Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

Energy Technology Data Exchange (ETDEWEB)

Albariño, César G., E-mail: calbarino@cdc.gov; Wiggleton Guerrero, Lisa; Lo, Michael K.; Nichol, Stuart T.; Towner, Jonathan S.

2015-10-15

Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014–2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. - Highlights: • Recombinant Ebola virus (EBOV) derived from Makona variant was rescued. • New protocol for viral rescue allows 100% efficiency. • Modified EBOV expresses a green fluorescent protein from a VP40-fused protein. • Modified EBOV was tested as tool to screen antiviral compounds and measure neutralizing antibodies.

Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

International Nuclear Information System (INIS)

Albariño, César G.; Wiggleton Guerrero, Lisa; Lo, Michael K.; Nichol, Stuart T.; Towner, Jonathan S.

2015-01-01

Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014–2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. - Highlights: • Recombinant Ebola virus (EBOV) derived from Makona variant was rescued. • New protocol for viral rescue allows 100% efficiency. • Modified EBOV expresses a green fluorescent protein from a VP40-fused protein. • Modified EBOV was tested as tool to screen antiviral compounds and measure neutralizing antibodies

Acquisition system testing with superfluid helium

International Nuclear Information System (INIS)

Anderson, J.E.; Fester, D.A.; DiPirro, M.J.

1988-01-01

NASA is evaluating both a thermomechanical pump and centrifugal pump for the SHOOT experiment using capillary fluid acquisition systems. Tests were conducted for these systems with superfluid helium under adverse operating conditions. Minus one-g outflow tests were run in conjunction with the thermomechanical pump. Both fine mesh screen and porous sponges were tested. A screen acquisition device was also tested with the low-NPSH centrifugal pump. Results to date show that the screen and sponge are capable of supplying superfluid helium to the thermomechanical pump inlet against a one-g head up to four cm. This is more than sufficient for the SHOOT application. Results with the sponge were reproducible while those with the screen could not always be repeated

Radioisotope Thermoelectric Generator Transporation System licensed hardware second certification test series and package shock mount system test

International Nuclear Information System (INIS)

Ferrell, P.C.; Moody, D.A.

1995-10-01

This paper presents a summary of two separate drop test a e performed in support of the Radioisotope Thermoelectric Generator (RTG) Transportation System (RTGTS). The first portion of this paper presents the second series of drop testing required to demonstrate that the RTG package design meets the requirements of Title 10, Code of Federal Regulations, ''Part 71'' (10 CFR 71). Results of the first test series, performed in July 1994, demonstrated that some design changes were necessary. The package design was modified to improve test performance and the design changes were incorporated into the Safety Analysis Report for Packaging (SARP). The second full-size certification test article (CTA-2) incorporated the modified design and was tested at the US Department of Energy's (DOE) Hanford Site near Richland, Washington. With the successful completion of the test series, and pending DOE Office of Facility Safety Analysis approval of the SARP, a certificate of compliance will be issued for the RTG package allowing its use. The second portion of this paper presents the design and testing of the RTG Package Mount System. The RTG package mount was designed to protect the RTG from excessive vibration during transport, provide shock protection during on/off loading, and provide a mechanism for moving the RTG package with a forklift. Military Standard (MIL-STD) 810E, Transit Drop Procedure (DOE 1989), was used to verify that the shock limiting system limited accelerations in excess of 15 G's at frequencies below 150 Hz. Results of the package mount drop tests indicate that an impact force of 15 G's was not exceeded in any test from a free drop height of 457 mm (18 in.)

Tetracycline-inducible system for regulation of skeletal muscle-specific gene expression in transgenic mice

Science.gov (United States)

Grill, Mischala A.; Bales, Mark A.; Fought, Amber N.; Rosburg, Kristopher C.; Munger, Stephanie J.; Antin, Parker B.

2003-01-01

Tightly regulated control of over-expression is often necessary to study one aspect or time point of gene function and, in transgenesis, may help to avoid lethal effects and complications caused by ubiquitous over-expression. We have utilized the benefits of an optimized tet-on system and a modified muscle creatine kinase (MCK) promoter to generate a skeletal muscle-specific, doxycycline (Dox) controlled over-expression system in transgenic mice. A DNA construct was generated in which the codon optimized reverse tetracycline transactivator (rtTA) was placed under control of a skeletal muscle-specific version of the mouse MCK promoter. Transgenic mice containing this construct expressed rtTA almost exclusively in skeletal muscles. These mice were crossed to a second transgenic line containing a bi-directional promoter centered on a tet responder element driving both a luciferase reporter gene and a tagged gene of interest; in this case the calpain inhibitor calpastatin. Compound hemizygous mice showed high level, Dox dependent muscle-specific luciferase activity often exceeding 10,000-fold over non-muscle tissues of the same mouse. Western and immunocytochemical analysis demonstrated similar Dox dependent muscle-specific induction of the tagged calpastatin protein. These findings demonstrate the effectiveness and flexibility of the tet-on system to provide a tightly regulated over-expression system in adult skeletal muscle. The MCKrtTA transgenic lines can be combined with other transgenic responder lines for skeletal muscle-specific over-expression of any target gene of interest.

Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Science.gov (United States)

Fujiwara, Kei; Katayama, Tsutomu; Nomura, Shin-ichiro M.

2013-01-01

Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription–translation–replication process by autonomous expression of chromosomal DNA replication machineries from a reconstituted transcription–translation system (PURE system). We found that low temperature is essential to express a complex protein, DNA polymerase III, in a single tube using the PURE system. Addition of the 13 genes, encoding initiator, DNA helicase, helicase loader, RNA primase and DNA polymerase III to the PURE system gave rise to a DNA replication system by a coupling manner. An artificial genetic circuit demonstrated that the DNA produced as a result of the replication is able to provide genetic information for proteins, indicating the in vitro central dogma can sequentially undergo two rounds. PMID:23737447

21 CFR 862.1230 - Cyclic AMP test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Cyclic AMP test system. 862.1230 Section 862.1230....1230 Cyclic AMP test system. (a) Identification. A cyclic AMP test system is a device intended to measure the level of adenosine 3′, 5′-monophosphate (cyclic AMP) in plasma, urine, and other body fluids...

Nisin-induced Expression of Pediocin in Dairy Lactic Acid Bacteria

Science.gov (United States)

To test if a single vector, nisin-controlled expression (NICE) system could be used to regulate expression of the pediocin operon in Streptococcus thermophilus, Lactococcus lactis subsp. lactis and Lactobacillus casei, the intact pediocin operon was cloned into pMSP3535 immediately down stream of th...

Automated real-time testing (ARTT) for embedded control systems (ECS)

International Nuclear Information System (INIS)

Hawkins, J; Howard, R; Nguyen, H.

2001-01-01

Many of today's automated real-time testing systems for embedded systems were developed using expensive custom hardware and software. In this article they describe how to use commercially available off-the-shelf hardware and software to design and develop an automated real-time test systems for Embedded Programmable Logic Controller (PLC) Based Control Systems. The system development began with the implementation of the VALI/TEST Pro testing methodology as a means for structuring the testing. Using this methodology, they were able to decompose system requirement documents for a Personnel Safety System (PSS) into its high, intermediate and detail level requirements. next, the validation procedures for the PSS system were decomposed into testing units called builds, test runs and test cases. To measure the PSS system's test coverage three levels of system requirements were mapped to their respective unit level of test using a specially constructed validation matrix that was designed to handle over 150 test cases and requirements. All of the above work led to the development of an Automated Real-Time Test System (ARTTS) that is capable of performing complete black box testing in real-time for Embedded PLC Based Control Systems. Also note, that the PSS system under test and mentioned in this paper is located at the Advance Photon Source (APS) at Argonne National Laboratory Basic Energy Science Facility in Argonne, Illinois

BRCA1 and BRCA2 expression patterns and prognostic significance in digestive system cancers.

Science.gov (United States)

Wang, Gui-Hua; Zhao, Chun-Mei; Huang, Ying; Wang, Wei; Zhang, Shu; Wang, Xudong

2018-01-01

The role of BRCA1 and BRCA2 genes is mainly to maintain genome integrity in response to DNA damage through different mechanisms. Deregulation of BRCA1 and BRCA2 is associated with the development of tumor and altered sensitivity to chemotherapeutic agents. In this study, we determined protein expression of BRCA1 and BRCA2 in 4 digestive system cancers (gastric cancer, colorectal cancer, hepatocellular carcinoma, and pancreatic cancer) by immunohistochemistry on tissue microarrays. A total of 1546 samples of 4 types of cancer tissues, their matched adjacent nontumor tissues, and corresponding benign tissues were studied, respectively. Immunohistochemistry expression patterns of the 2 proteins and their correlation with patients' clinical parameters and overall survival were analyzed. The results showed that low expression of cytoplasmic BRCA1 and BRCA2 was commonly associated with advanced tumor-lymph node-metastasis stage, whereas high expression of nuclear BRCA1 was generally correlated with advanced tumor stages in these cancers. High expression of cytoplasmic BRCA1 and BRCA2 had significantly favorable overall survival in digestive system cancers; in contrast, BRCA1 nuclear expression usually predicted poor outcomes. We conclude that BRCA1 and BRCA2 could be used as clinicopathological biomarkers to evaluate the prognosis of digestive system cancers. Copyright © 2017 Elsevier Inc. All rights reserved.

Qualification and testing of CT systems

DEFF Research Database (Denmark)

Bartscher, Markus; Neuschaefer-Rube, Ulrich; Illemann, Jens

2018-01-01

This chapter focuses on system verification and conformance to specifications. System qualification is carried out to ensure that the system and itscomponents achieve the best performance—usually corresponding to the specificationsmade by the manufacturer. Acceptance and reverification testing ar...

Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.

Science.gov (United States)

Gagoski, Dejan; Mureev, Sergey; Giles, Nichole; Johnston, Wayne; Dahmer-Heath, Mareike; Škalamera, Dubravka; Gonda, Thomas J; Alexandrov, Kirill

2015-02-10

Although numerous techniques for protein expression and production are available the pace of genome sequencing outstrips our ability to analyze the encoded proteins. To address this bottleneck, we have established a system for parallelized cloning, DNA production and cell-free expression of large numbers of proteins. This system is based on a suite of pCellFree Gateway destination vectors that utilize a Species Independent Translation Initiation Sequence (SITS) that mediates recombinant protein expression in any in vitro translation system. These vectors introduce C or N terminal EGFP and mCherry fluorescent and affinity tags, enabling direct analysis and purification of the expressed proteins. To maximize throughput and minimize the cost of protein production we combined Gateway cloning with Rolling Circle DNA Amplification. We demonstrate that as little as 0.1 ng of plasmid DNA is sufficient for template amplification and production of recombinant human protein in Leishmania tarentolae and Escherichia coli cell-free expression systems. Our experiments indicate that this approach can be applied to large gene libraries as it can be reliably performed in multi-well plates. The resulting protein expression pipeline provides a valuable new tool for applications of the post genomic era. Copyright © 2014 Elsevier B.V. All rights reserved.

N-glycan sialylation in a silkworm-baculovirus expression system.

Science.gov (United States)

Suganuma, Masatoshi; Nomura, Tsuyoshi; Higa, Yukiko; Kataoka, Yukiko; Funaguma, Shunsuke; Okazaki, Hironobu; Suzuki, Takeo; Fujiyama, Kazuhito; Sezutsu, Hideki; Tatematsu, Ken-Ichiro; Tamura, Toshiki

2018-02-09

A silkworm-baculovirus system is particularly effective for producing recombinant proteins, including glycoproteins. However, N-glycan structures in silkworm differ from those in mammals. Glycoproteins in silkworm are secreted as pauci-mannose type N-glycans without sialic acid or galactose residues. Sialic acid on N-glycans plays important roles in protein functions. Therefore, we developed pathways for galactosylation and sialylation in silkworm. Sialylated N-glycans on proteins were successfully produced in silkworm by co-expressing galactosyltransferase and sialyltransferase and providing an external supply of a sialylation-related substrate. α2,3/α2,6 Sialylation to N-glycans was controlled by changing the type of sialyltransferase expressed in silkworm. Furthermore, the co-expression of N-acetylglucosaminyltransferase II facilitated the formation of additional di-sialylated N-glycan structures. Our results provide new information on the control of N-glycosylation in silkworm. Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

A Chimeric NaV1.8 Channel Expression System Based on HEK293T Cell Line

Directory of Open Access Journals (Sweden)

Xi Zhou

2018-04-01

Full Text Available Among the nine voltage-gated sodium channel (NaV subtypes, NaV1.8 is an attractive therapeutic target for pain. The heterologous expression of recombinant NaV1.8 currents is of particular importance for its electrophysiological and pharmacological studies. However, NaV1.8 expresses no or low-level functional currents when transiently transfected into non-neuronal cell lines. The present study aims to explore the molecular determinants limiting its functional expression and accordingly establish a functional NaV1.8 expression system. We conducted screening analysis of the NaV1.8 intracellular loops by constructing NaV chimeric channels and confirmed that the NaV1.8 C-terminus was the only limiting factor. Replacing this sequence with that of NaV1.4, NaV1.5, or NaV1.7 constructed functional channels (NaV1.8/1.4L5, NaV1.8/1.5L5, and NaV1.8/1.7L5, respectively, which expressed high-level NaV1.8-like currents in HEK293T cells. The chimeric channel NaV1.8/1.7L5 displayed much faster inactivation of its macroscopic currents than NaV1.8/1.4L5 and NaV1.8/1.5L5, and it was the most similar to wild-type NaV1.8 expressed in ND7/23 cells. Its currents were very stable during repetitive depolarizations, while its repriming kinetic was different from wild-type NaV1.8. Most importantly, NaV1.8/1.7L5 pharmacologically resembled wild-type NaV1.8 as revealed by testing their susceptibility to two NaV1.8 selective antagonists, APETx-2 and MrVIB. NaV chimeras study showed that at least the domain 2 and domain 4 of NaV1.8 were involved in binding with APETx-2. Our study provided new insights into the function of NaV1.8 intracellular loops, as well as a reliable and convenient expression system which could be useful in NaV1.8 studies.

Kilowatt Isotope Power System. Phase I. System test report. 78-KIPS-33

International Nuclear Information System (INIS)

1978-01-01

The KIPS Ground Demonstration System (GDS) was designed to simulate, as closely as possible, a Flight System Conceptual Design (FSCD). No radiator was incorporated and electric heat sources were used in place of isotope heat sources. To minimize air in-leakage and to simulate heat losses associated with space operation, the system was operated in a vacuum chamber. Initial testing was performed on the development system which did not incorporate a high performance turbine or non-condensing configuration of the cold liquid passages in certain regenerator vapor regions. After testing of the development system and retrofit to the GDS configuration, which included improvements in the above two items, the GDS was installed in the test chamber. Testing to date showed the GDS configuration has demonstrated a system efficiency of greater than 15%. Satisfactory heat balances have been calculated on most system components, permitting evaluation of component performance. Certain performance deficiencies currently exist which prevented the 18% efficiency goal being attained. These can be corrected with further development

Multisignal detecting system of pile integrity testing

Science.gov (United States)

Liu, Zuting; Luo, Ying; Yu, Shihai

2002-05-01

The low strain reflection wave method plays a principal rule in the integrating detection of base piles. However, there are some deficiencies with this method. For example, there is a blind area of detection on top of the tested pile; it is difficult to recognize the defects at deep-seated parts of the pile; there is still the planar of 3D domino effect, etc. It is very difficult to solve these problems only with the single-transducer pile integrity testing system. A new multi-signal piles integrity testing system is proposed in this paper, which is able to impulse and collect signals on multiple points on top of the pile. By using the multiple superposition data processing method, the detecting system can effectively restrain the interference and elevate the precision and SNR of pile integrity testing. The system can also be applied to the evaluation of engineering structure health.

TMACS Test Procedure TP007: System administration

International Nuclear Information System (INIS)

Scanlan, P.; Washburn, S.; Seghers, R.

1994-01-01

The TMACS Software Project Test Procedures translate the project's acceptance criteria into test steps. Software releases are certified when the affected Test Procedures are successfully performed and the customers authorize installation of these changes. This Test Procedure tests the TMACS System Administration functions

Study on service test procedure for SMES systems

Energy Technology Data Exchange (ETDEWEB)

Ren, L., E-mail: rl8868@126.co [R and D Center of Applied Superconductivity, Huazhong University of Science and Technology, Wuhan 430074 (China); Tang, Y.; Li, J.; Shi, J. [R and D Center of Applied Superconductivity, Huazhong University of Science and Technology, Wuhan 430074 (China); Hu, Y. [State Grid Electric Power Research Institute, Wuhan 430074 (China); Guo, F.; Chen, L.; Cheng, S. [R and D Center of Applied Superconductivity, Huazhong University of Science and Technology, Wuhan 430074 (China)

2009-10-15

Some superconducting magnetic energy storage (SMES) systems have been developed successfully, and the function of them was verified by experiment. Considering the impact on the system security and stability when they are connected to the power system, it is important to build a standard test procedure and propose the main experimental contents for some representative superconducting devices. Based on the experimental results of a 35 kJ/7 kW conduction-cooled high-T{sub c} SMES system, which was developed by Huazhong University of Science and Technology, the test items and methods for a conduction-cooled high-T{sub c} SMES system are studied. For performance evaluation of this high-T{sub c} SMES, three superconducting magnet performance tests, power conditioning characteristic test and dynamic experiment in a simulated power system were fulfilled.

Study on service test procedure for SMES systems

International Nuclear Information System (INIS)

Ren, L.; Tang, Y.; Li, J.; Shi, J.; Hu, Y.; Guo, F.; Chen, L.; Cheng, S.

2009-01-01

Some superconducting magnetic energy storage (SMES) systems have been developed successfully, and the function of them was verified by experiment. Considering the impact on the system security and stability when they are connected to the power system, it is important to build a standard test procedure and propose the main experimental contents for some representative superconducting devices. Based on the experimental results of a 35 kJ/7 kW conduction-cooled high-T c SMES system, which was developed by Huazhong University of Science and Technology, the test items and methods for a conduction-cooled high-T c SMES system are studied. For performance evaluation of this high-T c SMES, three superconducting magnet performance tests, power conditioning characteristic test and dynamic experiment in a simulated power system were fulfilled.

Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Directory of Open Access Journals (Sweden)

Kentaro Hiraoka

2017-06-01

Full Text Available The tail suspension test (TST has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part, ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part. The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to

Preoperational test report, primary ventilation condensate system

Energy Technology Data Exchange (ETDEWEB)

Clifton, F.T.

1997-01-29

Preoperational test report for Primary Ventilation Condensate System, Project W-030. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. The system provides a collection point for condensate generated by the W-030 primary vent offgas cooling system serving tanks AYIOI, AY102, AZIOI, AZI02. The system is located inside a shielded ventilation equipment cell and consists of a condensate seal pot, sampling features, a drain line to existing Catch Tank 241-AZ-151, and a cell sump jet pump. The tests verify correct system operation and correct indications displayed by the central Monitor and Control System.

Fermilab tevatron five refrigerator system tests

International Nuclear Information System (INIS)

Rode, C.; Ferry, R.; Leiniger, M.; Makara, J.; Misek, J.; Mizicko, D.; Richied, D.; Theilacker, J.

1982-01-01

The Fermilab Tevatron refrigeration system is described with the layout illustrated. The compressor control loops, the refrigerator control loops, and magnet control loops (two per refrigerator) are described and each illustrated. The mobile purifier is described. A five refrigerator test is presented, using two compressor buildings, satellite refrigerator concept test and the test current to the writing. The configuration of the five refrigerator test is diagramed

INEL test plan for evaluating waste assay systems

International Nuclear Information System (INIS)

Mandler, J.W.; Becker, G.K.; Harker, Y.D.; Menkhaus, D.E.; Clements, T.L. Jr.

1996-09-01

A test bed is being established at the Idaho National Engineering Laboratory (INEL) Radioactive Waste Management Complex (RWMC). These tests are currently focused on mobile or portable radioassay systems. Prior to disposal of TRU waste at the Waste Isolation Pilot Plant (WIPP), radioassay measurements must meet the quality assurance objectives of the TRU Waste Characterization Quality Assurance Program Plan. This test plan provides technology holders with the opportunity to assess radioassay system performance through a three-tiered test program that consists of: (a) evaluations using non-interfering matrices, (b) surrogate drums with contents that resemble the attributes of INEL-specific waste forms, and (c) real waste tests. Qualified sources containing a known mixture and range of radionuclides will be used for the non-interfering and surrogate waste tests. The results of these tests will provide technology holders with information concerning radioassay system performance and provide the INEL with data useful for making decisions concerning alternative or improved radioassay systems that could support disposal of waste at WIPP

INEL test plan for evaluating waste assay systems

Energy Technology Data Exchange (ETDEWEB)

Mandler, J.W.; Becker, G.K.; Harker, Y.D.; Menkhaus, D.E.; Clements, T.L. Jr.

1996-09-01

A test bed is being established at the Idaho National Engineering Laboratory (INEL) Radioactive Waste Management Complex (RWMC). These tests are currently focused on mobile or portable radioassay systems. Prior to disposal of TRU waste at the Waste Isolation Pilot Plant (WIPP), radioassay measurements must meet the quality assurance objectives of the TRU Waste Characterization Quality Assurance Program Plan. This test plan provides technology holders with the opportunity to assess radioassay system performance through a three-tiered test program that consists of: (a) evaluations using non-interfering matrices, (b) surrogate drums with contents that resemble the attributes of INEL-specific waste forms, and (c) real waste tests. Qualified sources containing a known mixture and range of radionuclides will be used for the non-interfering and surrogate waste tests. The results of these tests will provide technology holders with information concerning radioassay system performance and provide the INEL with data useful for making decisions concerning alternative or improved radioassay systems that could support disposal of waste at WIPP.

Routine testing on protective and safety systems and components

International Nuclear Information System (INIS)

Rysy, W.

1977-01-01

1) In-process inspection, tests during commissioning. 2) Tests during reactor operation. 2.1) Reactor protection system, for example: continuous auto-testing by a dynamic system, check of the output signals; 2.2) safety features: selected examples: functional tests on the ECCS, trial operation of the emergency diesels. 3) Tests during refuelling phase. 3.1) Containment: Leakage rate tests, leak testing; 3.2) coolant system: selected examples: inservice inspections of the pressure vessel, eddy current testing of the steam generator, functional tests of safety valves. (orig./HP) [de

Powertrain instrumentation and test systems development, hybridization, electrification

CERN Document Server

Paulweber, Michael

2016-01-01

The book deals with the increasingly complex test systems for powertrain components and systems giving an overview of the diverse types of test beds for all components of an advanced powertrain focusing on specific topics such as instrumentation, control, simulation, hardware-in-the-loop, automation or test facility management. This book is intended for powertrain (component) development engineers, test bed planners, test bed operators and beginners.

PUREX exhaust ventilation system installation test report

International Nuclear Information System (INIS)

Blackaby, W.B.

1997-01-01

This Acceptance Test Report validates the testing performed, the exceptions logged and resolved and certifies this portion of the SAMCONS has met all design and test criteria to perform as an operational system. The proper installation of the PUREX exhaust ventilation system components and wiring was systematically evaluated by performance of this procedure. Proper operation of PUREX exhaust fan inlet, outlet, and vortex damper actuators and limit switches were verified, using special test equipment, to be correct and installed wiring connections were verified by operation of this equipment

Generic test platform for representative tests of safety I/C systems - 15546

International Nuclear Information System (INIS)

Fourestie, B.; Kuck, H.; Richter, J.; Rieche, S.; Waitz, M.

2015-01-01

In compliance with the IEC 61513 safety Instrumentation and Control (I/C) systems must be successfully validated in their final configuration prior to installation on site and commissioning. However the contingent need for modifications during system validation activities or subsequently during the commissioning phase may entail long and costly re-engineering of the I/C systems. With the view to ease these possible modifications, a Generic Test Platform has been developed by AREVA which allows combining a real I/C system subpart with an emulation server. This platform provides a faithful representation of the I/C System allowing crediting the validation test results carried out on this platform. (authors)

Control of gene expression by CRISPR-Cas systems

Science.gov (United States)

2013-01-01

Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems. PMID:24273648

Scheduling system for test automation framework

NARCIS (Netherlands)

Wahyudi, Djohan

2014-01-01

An Interventional X-ray (iXR) system provides real time X-ray imaging with high image clarity and low X-ray dose. After several years of development, the iXR System has become complex. In order to verify the correct operation of the system, the system integration and test group performs extensive

Verification test of control rod system for HTR-10

International Nuclear Information System (INIS)

Zhou Huizhong; Diao Xingzhong; Huang Zhiyong; Cao Li; Yang Nianzu

2002-01-01

There are 10 sets of control rods and driving devices in 10 MW High Temperature Gas-cooled Test Reactor (HTR-10). The control rod system is the controlling and shutdown system of HTR-10, which is designed for reactor criticality, operation, and shutdown. In order to guarantee technical feasibility, a series of verification tests were performed, including room temperature test, thermal test, test after control rod system installed in HTR-10, and test of control rod system before HTR-10 first criticality. All the tests data showed that driving devices working well, control rods running smoothly up and down, random position settling well, and exactly position indicating

Seismic proving test of process computer systems with a seismic floor isolation system

International Nuclear Information System (INIS)

Fujimoto, S.; Niwa, H.; Kondo, H.

1995-01-01

The authors have carried out seismic proving tests for process computer systems as a Nuclear Power Engineering Corporation (NUPEC) project sponsored by the Ministry of International Trade and Industry (MITI). This paper presents the seismic test results for evaluating functional capabilities of process computer systems with a seismic floor isolation system. The seismic floor isolation system to isolate the horizontal motion was composed of a floor frame (13 m x 13 m), ball bearing units, and spring-damper units. A series of seismic excitation tests was carried out using a large-scale shaking table of NUPEC. From the test results, the functional capabilities during large earthquakes of computer systems with a seismic floor isolation system were verified

Performance testing SPECT systems: Recent developments

International Nuclear Information System (INIS)

Todd-Pokropek, A.

1985-01-01

A protocol is used to test as many currently available systems as possible, including both rotating gamma camera systems from GE, Siemens, Toshiba, Technicare, Elscint, Philips, Picker, etc, and single/multi slice system such as the Medimatic. This paper indicates changes that have occurred with respect to preliminary results previously published. Reconstructions were performed in a standard manner, using a Ramp filter and without attenuation correction, in order to reduce the effect of differences in software. The Jaszczak phantom was used to assess overall image quality. Tests were performed using the different collimators available. Image quality improved with better spatial resolution, even at the expense of sensitivity. Non-circular orbits, where available, were tested, and resolution was comparing for various positions and directions. With double headed systems, the results obtained were compared to those using a single head alone. Some gain in image quality seems to have been achieved by better uniformity resulting from improved correction circuitry, by the use of non-circular orbits, and from scatter correction. Potential still exists to improve collimator design

Major vault protein/lung resistance-related protein (MVP/LRP) expression in nervous system tumors.

Science.gov (United States)

Sasaki, Tsutomu; Hankins, Gerald R; Helm, Gregory A

2002-01-01

Lung resistance-related protein (LRP) was identified as the major vault protein (MVP), the main component of multimeric vault particles. It functions as a transport-associated protein that can be associated with multidrug resistance. In previous studies, expression of MVP/LRP has been documented in tumors of various types. In general, good correlations have been reported for expression of MVP/LRP and decreased sensitivity to chemotherapy and poor prognosis. MVP/LRP expression has been documented in glioblastomas, but its expression in nervous system tumors in general has not been well characterized. Immunohistochemistry using anti-human MVP/LRP antibody (LRP-56) was performed on formalin-fixed, paraffin-embedded archival tissue from 69 primary central nervous system tumors. Expression of MVP/LRP was observed in 81.2% (56/69) of primary nervous system tumors, including astrocytomas (11/13), oligodendrogliomas (1/2), oligoastrocytomas (5/5), ependymoma (1/1), meningiomas (35/45), schwannomas (2/2), and neurofibroma (1/1). Various degrees and distributions of immunoreactivity to MVP/ LRP were observed. Neither the presence nor the degree of immunoreactivity to MVP/LRP showed any correlation with either tumor grade or the presence of brain invasion.

Data acquisition and test system software

International Nuclear Information System (INIS)

Bourgeois, N.A. Jr.

1979-03-01

Sandia Laboratories has been assigned the task by the Base and Installation Security Systems (BISS) Program Office to develop various aspects of perimeter security systems. One part of this effort involves the development of advanced signal processing techniques to reduce the false and nuisance alarms from sensor systems while improving the probability of intrusion detection. The need existed for both data acquisition hardware and software. Also, the hardware is used to implement and test the signal processing algorithms in real time. The hardware developed for this signal processing task is the Data Acquisition and Test System (DATS). The programs developed for use on DATS are described. The descriptions are taken directly from the documentation included within the source programs themselves

A game-theoretic approach to real-time system testing

DEFF Research Database (Denmark)

David, Alexandre; Larsen, Kim Guldstrand; Li, Shuhao

2008-01-01

This paper presents a game-theoretic approach to the testing of uncontrollable real-time systems. By modelling the systems with Timed I/O Game Automata and specifying the test purposes as Timed CTL formulas, we employ a recently developed timed game solver UPPAAL-TIGA to synthesize winning...... strategies, and then use these strategies to conduct black-box conformance testing of the systems. The testing process is proved to be sound and complete with respect to the given test purposes. Case study and preliminary experimental results indicate that this is a viable approach to uncontrollable timed...... system testing....

Initial test results of the Omron face cue entry system at the University of Missouri-Rolla Reactor

International Nuclear Information System (INIS)

Tokuhiro, Akira T.; Vaughn, Brian J.

2004-01-01

The University of Missouri-Rolla Reactor facility is testing, in collaboration with Omron Transaction Systems, Inc., the Omron Face Cue facial recognition system for access control to its restricted area. The installation of this system is the first of its kind at a security-relevant facility in the U.S. and within the research reactor community. The Face Cue is an on-demand device based on facial recognition and storage technology. The image processing methodology is as follows: (1) facial position detection, (2) background elimination, (3) facial features discrimination via application of a wavelet transform. The extracted facial feature values are compared to the data archived in its database and access is provided upon meeting the authorization criteria. The current test phase consisted of assessing the functionality of the Face Cue during daily use and in terms of its robustness (flexibility) as a function of the following physical parameters: (1) subject's distance away from the Face Cue, (2) ambient lighting conditions, (3) subject's facial orientation, (4) subject's facial expression and (5) peripheral facial features/modifications. The system has operated at nearly 100% reliability during several test intervals with approximately 7,000 entry attempts to date. (author)

A Novel Tightly Regulated Gene Expression System for the Human Intestinal Symbiont Bacteroides thetaiotaomicron

Directory of Open Access Journals (Sweden)

Regis Stentz

2016-07-01

Full Text Available There is considerable interest in studying the function of Bacteroides species resident in the human gastrointestinal (GI-tract and the contribution they make to host health. Reverse genetics and protein expression techniques, such as those developed for well-characterised Escherichia coli cannot be applied to Bacteroides species as they and other members of the Bacteriodetes phylum have unique promoter structures. The availability of useful Bacteroides-specific genetic tools is therefore limited. Here we describe the development of an effective mannan-controlled gene expression system for Bacteroides thetaiotaomicron containing the mannan-inducible promoter–region of an α-1,2-mannosidase gene (BT_3784, a ribosomal binding site designed to modulate expression, a multiple cloning site to facilitate the cloning of genes of interest, and a transcriptional terminator. Using the Lactobacillus pepI as a reporter gene, mannan induction resulted in an increase of reporter activity in a time- and concentration-dependent manner with a wide range of activity. The endogenous BtcepA cephalosporinase gene was used to demonstrate the suitability of this novel expression system, enabling the isolation of a His-tagged version of BtCepA. We have also shown with experiments performed in mice that the system can be induced in vivo in the presence of an exogenous source of mannan. By enabling the controlled expression of endogenous and exogenous genes in B. thetaiotaomicron this novel inducer-dependent expression system will aid in defining the physiological role of individual genes and the functional analyses of their products.

Circulating microRNA expression profiles associated with systemic lupus erythematosus

DEFF Research Database (Denmark)

Carlsen, Anting Liu; Schetter, Aaron J; Nielsen, Christoffer

2013-01-01

OBJECTIVE: To evaluate the specificity of expression patterns of cell-free, circulating microRNAs in systemic lupus erythematosus (SLE). METHODS: Total RNA was purified from plasma and 45 different specific mature microRNAs were determined using quantitative reverse transcription polymerase chain...

Test-Access Planning and Test Scheduling for Embedded Core-Based System Chips

OpenAIRE

Goel, Sandeep Kumar

2005-01-01

Advances in the semiconductor process technology enable the creation of a complete system on one single die, the so-called system chip or SOC. To reduce time-to-market for large SOCs, reuse of pre-designed and pre-veried blocks called cores is employed. Like the design style, testing of SOCs can be best approached in a core-based fashion. In order to enable core-based test development, an embedded core should be isolated from its surrounding circuitry and electrical test access from chip pins...

Methodological approaches to conducting pilot and proof tests on reverse-osmosis systems: Results of comparative studies

Science.gov (United States)

Panteleev, A. A.; Bobinkin, V. V.; Larionov, S. Yu.; Ryabchikov, B. E.; Smirnov, V. B.; Shapovalov, D. A.

2017-10-01

When designing large-scale water-treatment plants based on reverse-osmosis systems, it is proposed to conduct experimental-industrial or pilot tests for validated simulation of the operation of the equipment. It is shown that such tests allow establishing efficient operating conditions and characteristics of the plant under design. It is proposed to conduct pilot tests of the reverse-osmosis systems on pilot membrane plants (PMPs) and test membrane plants (TMPs). The results of a comparative experimental study of pilot and test membrane plants are exemplified by simulating the operating parameters of the membrane elements of an industrial plant. It is concluded that the reliability of the data obtained on the TMP may not be sufficient to design industrial water-treatment plants, while the PMPs are capable of providing reliable data that can be used for full-scale simulation of the operation of industrial reverse-osmosis systems. The test membrane plants allow simulation of the operating conditions of individual industrial plant systems; therefore, potential areas of their application are shown. A method for numerical calculation and experimental determination of the true selectivity and the salt passage are proposed. An expression has been derived that describes the functional dependence between the observed and true salt passage. The results of the experiments conducted on a test membrane plant to determine the true value of the salt passage of a reverse-osmosis membrane are exemplified by magnesium sulfate solution at different initial operating parameters. It is shown that the initial content of a particular solution component has a significant effect on the change in the true salt passage of the membrane.

Caustic addition system operability test procedure

International Nuclear Information System (INIS)

Parazin, R.E.

1994-11-01

This test procedure provides instructions for performing operational testing of the major components of the 241-AN-107 Caustic Addition System by WHC and Kaiser personnel at the Rotating Equipment Shop run-in pit (Bldg. 272E)

Caustic addition system operability test procedure

Energy Technology Data Exchange (ETDEWEB)

Parazin, R.E.

1994-11-01

This test procedure provides instructions for performing operational testing of the major components of the 241-AN-107 Caustic Addition System by WHC and Kaiser personnel at the Rotating Equipment Shop run-in pit (Bldg. 272E).

Deregulated HOXB7 expression predicts poor prognosis of patients with malignancies of digestive system.

Science.gov (United States)

Liu, Fang-Teng; Chen, Han-Min; Xiong, Ying; Zhu, Zheng-Ming

2017-07-26

Numerous studies have investigated the relationship between deregulated HOXB7 expression with the clinical outcome in patients with digestive stem cancers, HOXB7 has showed negative impacts but with varying levels. We aimed to comprehensively evaluate the prediction and prognostic value of HOXB7 in digestive stem cancers. Electronic databases updated to December 1, 2016 were retrieved to collect relevant eligible studies to quantitatively explore the potential roles of HOXB7 as a prognostic indicator in digestive system cancers. A total of 9 studies (n = 1298 patients) was included in this synthetical meta-analysis. The pooled hazard ratios suggested that high expression of HOXB7 protein was associated with poor prognosis of OS in patients with digestive system cancers (HR = 1.97, 95% CI: 1.65-2.28, p= 0.000), and HOXB7 protein could act as an independent prognostic factor for predicting OS of patients with digestive system cancers (HR: 2.02, 95% CI: 1.69-2.36, p = 0.000). Statistical significance was also observed in subgroup meta-analysis based on the cancer type, histology type, country, sample size and publication date. Furthermore, we examined the correlations between HOXB7 protein and clinicopathological features. It showed that altered expression of HOXB7 protein was correlated with tumor invasion (p = 0.000), lymph node status (p = 0.000), distant metastasis (p = 0.001) and TNM stage (p = 0.000). However, the expression of HOXB7 protein was not associated with age (p = 0.64), gender (p = 0.40) or levels of differentiation (p = 0.19). High expression of HOXB7 protein was associated with poor prognosis of patients with digestive system cancers, as well as clinicopathologic characteristics, including the tumor invasion, lymph node status, distant metastasis and TNM stage. The expression of HOXB7 protein was not associated with age, gender or levels of differentiation. HOXB7 protein expression level in tumor tissue might serve as a novel prognostic marker for

Software Sub-system in Loading Automatic Test System for the Measurement of Power Line Filters

Directory of Open Access Journals (Sweden)

Yu Bo

2017-01-01

Full Text Available The loading automatic test system for measurement of power line filters are in urgent demand. So the software sub-system of the whole test system was proposed. Methods: structured the test system based on the virtual instrument framework, which consisted of lower and up computer and adopted the top down approach of design to perform the system and its modules, according to the measurement principle of the test system. Results: The software sub-system including human machine interface, data analysis and process software, expert system, communication software, control software in lower computer, etc. had been designed. Furthermore, it had been integrated into the entire test system. Conclusion: This sub-system provided a fiendly software platform for the whole test system, and had many advantages such as strong functions, high performances, low prices. It not only raises the test efficiency of EMI filters, but also renders some creativities.

Systemic distribution, subcellular localization and differential expression of sphingosine-1-phosphate receptors in benign and malignant human tissues.

Science.gov (United States)

Wang, Chunyi; Mao, Jinghe; Redfield, Samantha; Mo, Yinyuan; Lage, Janice M; Zhou, Xinchun

2014-10-01

Five sphingosine-1-phosphate receptors (S1PR): S1PR1, S1PR2, S1PR3, S1PR4 and S1PR5 (S1PR1-5) have been shown to be involved in the proliferation and progression of various cancers. However, none of the S1PRs have been systemically investigated. In this study, we performed immunohistochemistry (IHC) for S1PR1-S1PR5 on different tissues, in order to simultaneously determine the systemic distribution, subcellular localization and expression level of all five S1PRs. We constructed tissue microarrays (TMAs) from 384 formalin-fixed paraffin-embedded (FFPE) blocks containing 183 benign and 201 malignant tissues from 34 human organs/systems. Then we performed IHC for all five S1PRs simultaneously on these TMA slides. The distribution, subcellular localization and expression of each S1PR were determined for each tissue. The data in benign and malignant tissues from the same organ/tissue were then compared using the Student's t-test. In order to reconfirm the subcellular localization of each S1PR as determined by IHC, immunocytochemistry (ICC) was performed on several malignant cell lines. We found that all five S1PRs are widely distributed in multiple human organs/systems. All S1PRs are expressed in both the cytoplasm and nucleus, except S1PR3, whose IHC signals are only seen in the nucleus. Interestingly, the S1PRs are rarely expressed on cellular membranes. Each S1PR is unique in its organ distribution, subcellular localization and expression level in benign and malignant tissues. Among the five S1PRs, S1PR5 has the highest expression level (in either the nucleus or cytoplasm), with S1PR1, 3, 2 and 4 following in descending order. Strong nuclear expression was seen for S1PR1, S1PR3 and S1PR5, whereas S1PR2 and S1PR4 show only weak staining. Four organs/tissues (adrenal gland, liver, brain and colon) show significant differences in IHC scores for the multiple S1PRs (nuclear and/or cytoplasmic), nine (stomach, lymphoid tissues, lung, ovary, cervix, pancreas, skin, soft

21 CFR 862.1515 - Nitrogen (amino-nitrogen) test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Nitrogen (amino-nitrogen) test system. 862.1515... Systems § 862.1515 Nitrogen (amino-nitrogen) test system. (a) Identification. A nitrogen (amino-nitrogen) test system is a device intended to measure amino acid nitrogen levels in serum, plasma, and urine...

Dynamics of immune system gene expression upon bacterial challenge and wounding in a social insect (Bombus terrestris.

Directory of Open Access Journals (Sweden)

Silvio Erler

2011-03-01

Full Text Available The innate immune system which helps individuals to combat pathogens comprises a set of genes representing four immune system pathways (Toll, Imd, JNK and JAK/STAT. There is a lack of immune genes in social insects (e.g. honeybees when compared to Diptera. Potentially, this might be compensated by an advanced system of social immunity (synergistic action of several individuals. The bumble bee, Bombus terrestris, is a primitively eusocial species with an annual life cycle and colonies headed by a single queen. We used this key pollinator to study the temporal dynamics of immune system gene expression in response to wounding and bacterial challenge.Antimicrobial peptides (AMP (abaecin, defensin 1, hymenoptaecin were strongly up-regulated by wounding and bacterial challenge, the latter showing a higher impact on the gene expression level. Sterile wounding down-regulated TEP A, an effector gene of the JAK/STAT pathway, and bacterial infection influenced genes of the Imd (relish and JNK pathway (basket. Relish was up-regulated within the first hour after bacterial challenge, but decreased strongly afterwards. AMP expression following wounding and bacterial challenge correlates with the expression pattern of relish whereas correlated expression with dorsal was absent. Although expression of AMPs was high, continuous bacterial growth was observed throughout the experiment.Here we demonstrate for the first time the temporal dynamics of immune system gene expression in a social insect. Wounding and bacterial challenge affected the innate immune system significantly. Induction of AMP expression due to wounding might comprise a pre-adaptation to accompanying bacterial infections. Compared with solitary species this social insect exhibits reduced immune system efficiency, as bacterial growth could not be inhibited. A negative feedback loop regulating the Imd-pathway is suggested. AMPs, the end product of the Imd-pathway, inhibited the up-regulation of the

Supernatant treatment system design through testing

International Nuclear Information System (INIS)

Ploetz, D.K.; Leonard, I.M.

1988-12-01

The main purpose of the Supernatant Treatment System (STS) is to remove more than 99.9 percent of the radioactive cesium (Cs-137) from the high-level waste stored in tank 8D-2. Cesium removal is accomplished in the STS by processing the supernatant (liquid) portion of the high-level waste through three or four ion exchange columns filled with zeolite. After treatment in the STS, the decontaminated supernatant is processed as low-level waste and finally encapsulated in cement for eventual disposal. The Cs-137 removed from the waste and absorbed onto zeolite ion exchange material is temporarily stored in tank 8D-1 until it can be encapsulated in glass and disposed of as high-level waste. This report discusses construction and testing of the STS. Design of the STS was started in 1982 in parallel with the selection of the ion exchange material. The construction of this system was accomplished in five phase in parallel with completion of design to allow for faster completion of the project. The existing high-level waste storage tanks -- 8D-1, 8D-2, and 8D-3 -- required major renovations to permit transfer of the high-level waste from tank 8D-2 to tank 8D-1, to house the components that comprise the STS in tank 8D-1, and to store decontaminated wastes in tank 8D-3. Testing in the STS started before construction was complete and was accomplished by first testing components individually. Then the system was retested using simulated supernatant. Integrated testing of the whole Integrated Radwaste Treatment System (IRTS), which includes the STS, Liquid Waste Treatment System (LWTS), Cement Solidification System (CSS), and the Drum Cell, was also performed using simulated supernatant. Finally, slightly radioactive condensate water from tank 8D-1 was processed. After successfully completing this testing, the STS started operations with radioactive supernatant on May 23, 1988. 21 refs., 33 figs., 21 tabs

Test battery for measuring the perception and recognition of facial expressions of emotion

Science.gov (United States)

Wilhelm, Oliver; Hildebrandt, Andrea; Manske, Karsten; Schacht, Annekathrin; Sommer, Werner

2014-01-01

Despite the importance of perceiving and recognizing facial expressions in everyday life, there is no comprehensive test battery for the multivariate assessment of these abilities. As a first step toward such a compilation, we present 16 tasks that measure the perception and recognition of facial emotion expressions, and data illustrating each task's difficulty and reliability. The scoring of these tasks focuses on either the speed or accuracy of performance. A sample of 269 healthy young adults completed all tasks. In general, accuracy and reaction time measures for emotion-general scores showed acceptable and high estimates of internal consistency and factor reliability. Emotion-specific scores yielded lower reliabilities, yet high enough to encourage further studies with such measures. Analyses of task difficulty revealed that all tasks are suitable for measuring emotion perception and emotion recognition related abilities in normal populations. PMID:24860528

Test plan for qualification testing of the 241-SY-101 Flexible Receiver System

International Nuclear Information System (INIS)

Ritter, G.A.

1994-01-01

A mixer pump was installed in tank 241-SY-101 on July 3, 1993, to support hydrogen mitigation testing. This test mixer pump has proven to be very successful in mitigating the large gas releases, or ''burps,'' from the tank waste. Therefore, a decision has been made to fabricate a spare pump that will be installed upon failure of the existing test pump. Before replacement activities can be initiated, equipment must be available to remove the existing pump. A pump removal system is currently being designed and fabricated that will support the retrieval, transportation and disposal of the existing pump. The Equipment Removal System consists of six major components: the flexible receiver system (FRS), the equipment storage container, the container strongback system, the container transport trailer, the support cranes, and the pump washdown system

Facial Expression Recognition By Using Fisherface Methode With Backpropagation Neural Network

Directory of Open Access Journals (Sweden)

Zaenal Abidin

2011-01-01

Full Text Available Abstract— In daily lives, especially in interpersonal communication, face often used for expression. Facial expressions give information about the emotional state of the person. A facial expression is one of the behavioral characteristics. The components of a basic facial expression analysis system are face detection, face data extraction, and facial expression recognition. Fisherface method with backpropagation artificial neural network approach can be used for facial expression recognition. This method consists of two-stage process, namely PCA and LDA. PCA is used to reduce the dimension, while the LDA is used for features extraction of facial expressions. The system was tested with 2 databases namely JAFFE database and MUG database. The system correctly classified the expression with accuracy of 86.85%, and false positive 25 for image type I of JAFFE, for image type II of JAFFE 89.20% and false positive 15,  for type III of JAFFE 87.79%, and false positive for 16. The image of MUG are 98.09%, and false positive 5. Keywords— facial expression, fisherface method, PCA, LDA, backpropagation neural network.

The Challenges of Recombinant Endostatin in Clinical Application: Focus on the Different Expression Systems and Molecular Bioengineering

Directory of Open Access Journals (Sweden)

Abbas Mohajeri

2017-04-01

Full Text Available Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs is necessary in large scale. The production of rEs is difficult because of its structural properties and is high-cost. Therefore, this review focused on the different expression systems that involved in rEs production including; mammalian, baculovirus, yeast, and Escherichia coli (E. coli expression systems. The evaluating of the results of different expression systems declared that none of the mentioned systems can be considered to be generally superior to the other. Meanwhile with considering the advantages and disadvantage of E. coli expression system compared with other systems beside the molecular properties of Es, E. coli expression system can be a preferred expression system for expressing of the Es in large scale. Also, the molecular bioengineering and sustained release formulations that lead to improving of its stability and bioactivity will be discussed. Point mutation (P125A of Es, addition of RGD moiety or an additional zinc biding site to N-terminal of Es , fusing of Es to anti-HER2 IgG or heavy-chain of IgG, and finally loading of the endostar by PLGA and PEG- PLGA nanoparticles and gold nano-shell particles are the effective bioengineering methods to overcome to clinical changes of endostatin.

Testing and reference model analysis of FTTH system

Science.gov (United States)

Feng, Xiancheng; Cui, Wanlong; Chen, Ying

2009-08-01

With rapid development of Internet and broadband access network, the technologies of xDSL, FTTx+LAN , WLAN have more applications, new network service emerges in endless stream, especially the increase of network game, meeting TV, video on demand, etc. FTTH supports all present and future service with enormous bandwidth, including traditional telecommunication service, traditional data service and traditional TV service, and the future digital TV and VOD. With huge bandwidth of FTTH, it wins the final solution of broadband network, becomes the final goal of development of optical access network.. Fiber to the Home (FTTH) will be the goal of telecommunications cable broadband access. In accordance with the development trend of telecommunication services, to enhance the capacity of integrated access network, to achieve triple-play (voice, data, image), based on the existing optical Fiber to the curb (FTTC), Fiber To The Zone (FTTZ), Fiber to the Building (FTTB) user optical cable network, the optical fiber can extend to the FTTH system of end-user by using EPON technology. The article first introduced the basic components of FTTH system; and then explain the reference model and reference point for testing of the FTTH system; Finally, by testing connection diagram, the testing process, expected results, primarily analyze SNI Interface Testing, PON interface testing, Ethernet performance testing, UNI interface testing, Ethernet functional testing, PON functional testing, equipment functional testing, telephone functional testing, operational support capability testing and so on testing of FTTH system. ...

21 CFR 862.3080 - Breath nitric oxide test system.

Science.gov (United States)

2010-04-01

... Systems § 862.3080 Breath nitric oxide test system. (a) Identification. A breath nitric oxide test system... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Breath nitric oxide test system. 862.3080 Section... fractional nitric oxide concentration in expired breath aids in evaluating an asthma patient's response to...

21 CFR 862.1640 - Protein-bound iodine test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Protein-bound iodine test system. 862.1640 Section... Systems § 862.1640 Protein-bound iodine test system. (a) Identification. A protein-bound iodine test system is a device intended to measure protein-bound iodine in serum. Measurements of protein-bound...

Design and simulations for RFPI system test jig

International Nuclear Information System (INIS)

Keshwani, Rajesh; Khole, Shailesh; Sujo, C.I.; Shukla, Hitesh; Afaash, M.; Bharade, Sandeep; Joshi, Gopal

2015-01-01

RFPI system for condition monitoring and protection of RF systems of LEHIPA has been designed. The intention of this system is to protect and monitor different high power RF components. The system consists of variety of analog and digital cards to process, acquire and monitor signals from RF sensors, photo multiplier tubes (PMT), field emission probes, photo sensors, etc. The system can also accept and process digital inputs or contacts and analog input signals from other subsystems, indicating their status. It comprises of analog signal processing electronics, condition monitoring, data acquisition and fault reporting features. Since RFPI system should process and take action within prescribed time limit, the testing of system is critical issue. In order to carry out stand-alone field testing of such elaborate electronic system, variety of carefully designed electronic test circuit with suitable rangeability is required. These include fast current sources, fast amplifiers, fast rectifiers, etc. This paper elaborates on specifications, design approach, circuit design and simulations for various circuits used in test jig

Aviation Systems Test and Integration Lab (AvSTIL)

Data.gov (United States)

Federal Laboratory Consortium — The Aviation Systems Test and Integration Laboratory offers an innovative approach to aviation system and subsystem testing by fully immersing aviation platforms in...

Pelamis WEC - full-scale joint system test

Energy Technology Data Exchange (ETDEWEB)

Yemm, R.

2003-07-01

This report describes the building and testing of a full-scale Pelamis Wave Energy Converter (WEC) two-axis joint system using a laboratory joint test rig. The main project objective to develop an intermediate demonstration model to confirm full scale control, hydraulic and data acquisition systems is discussed, and the key objectives of the programme are listed. Details are given of the semi-submerged articulated structure of cylindrical elements linked by hinged joints, and the integrated testing of all key components. A summary of the work programme and a description of the test rig are presented.

Arbovirus vaccines: opportunities for the baculovirus-insect cell expression system

NARCIS (Netherlands)

Metz, S.W.H.; Pijlman, G.P.

2011-01-01

The baculovirus-insect cell expression system is a well-established technology for the production of heterologous viral (glyco)proteins in cultured cells, applicable for basic scientific research as well as for the development and production of vaccines and diagnostics. Arboviruses form an emerging

What is adapted in face adaptation? The neural representations of expression in the human visual system.

Science.gov (United States)

Fox, Christopher J; Barton, Jason J S

2007-01-05

The neural representation of facial expression within the human visual system is not well defined. Using an adaptation paradigm, we examined aftereffects on expression perception produced by various stimuli. Adapting to a face, which was used to create morphs between two expressions, substantially biased expression perception within the morphed faces away from the adapting expression. This adaptation was not based on low-level image properties, as a different image of the same person displaying that expression produced equally robust aftereffects. Smaller but significant aftereffects were generated by images of different individuals, irrespective of gender. Non-face visual, auditory, or verbal representations of emotion did not generate significant aftereffects. These results suggest that adaptation affects at least two neural representations of expression: one specific to the individual (not the image), and one that represents expression across different facial identities. The identity-independent aftereffect suggests the existence of a 'visual semantic' for facial expression in the human visual system.

QUASI-RANDOM TESTING OF COMPUTER SYSTEMS

Directory of Open Access Journals (Sweden)

S. V. Yarmolik

2013-01-01

Full Text Available Various modified random testing approaches have been proposed for computer system testing in the black box environment. Their effectiveness has been evaluated on the typical failure patterns by employing three measures, namely, P-measure, E-measure and F-measure. A quasi-random testing, being a modified version of the random testing, has been proposed and analyzed. The quasi-random Sobol sequences and modified Sobol sequences are used as the test patterns. Some new methods for Sobol sequence generation have been proposed and analyzed.

TTCN-3 for distributed testing embedded systems

NARCIS (Netherlands)

Blom, S.C.C.; Deiß, T.; Ioustinova, N.; Kontio, A.; Pol, van de J.C.; Rennoch, A.; Sidorova, N.; Virbitskaite, I.; Voronkov, A.

2007-01-01

Abstract. TTCN-3 is a standardized language for specifying and executing test suites that is particularly popular for testing embedded systems. Prior to testing embedded software in a target environment, the software is usually tested in the host environment. Executing in the host environment often

Railcar waste transfer system hydrostatic test report

International Nuclear Information System (INIS)

Ellingson, S.D.

1997-01-01

This Acceptance Test Report (ATR) documents for record purposes the field results, acceptance, and approvals of the completed acceptance test per HNF-SD-W417-ATP-001, ''Rail car Waste Transfer System Hydrostatic Test''. The test was completed and approved without any problems or exceptions

Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems

Energy Technology Data Exchange (ETDEWEB)

Conover, David R.; Crawford, Aladsair J.; Viswanathan, Vilayanur V.; Ferreira, Summer; Schoenwald, David

2014-06-01

The Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems (PNNL-22010) was first issued in November 2012 as a first step toward providing a foundational basis for developing an initial standard for the uniform measurement and expression of energy storage system (ESS) performance. Its subsequent use in the field and review by the protocol working group and most importantly the users’ subgroup and the thermal subgroup has led to the fundamental modifications reflected in this update of the 2012 Protocol. As an update of the 2012 Protocol, this document (the June 2014 Protocol) is intended to supersede its predecessor and be used as the basis for measuring and expressing ESS performance. The foreword provides general and specific details about what additions, revisions, and enhancements have been made to the 2012 Protocol and the rationale for them in arriving at the June 2014 Protocol.

Nanobarcode gene expression monitoring system for potential miniaturized space applications

Science.gov (United States)

Ruan, Weiming; Eastman, P. Scott; Cooke, Patrick A.; Park, Jennifer S.; Chu, Julia S. F.; Gray, Joe W.; Li, Song; Chen, Fanqing Frank

Manned mission to space has been threatened by various cosmos risks including radiation, mirogravity, vacuum, confinement, etc., which may cause genetic variations of astronauts and eventually lead to damages of their health. Thus, the development of small biomedical devices, which can monitor astronaut gene expression changes, is useful for future long-term space missions. Using magnetic microbeads packed with nanocrystal quantum dots at controlled ratios, we were able to generate highly multiplexed nanobarcodes, which can encode a flexible panel of genes. Also, by using a reporter quantum dot, this nanobarcode platform can monitor and quantify gene expression level with improved speed and sensitivity. As a comparison, we studied TGF-β1 induced transcription changes in human bone marrow mesenchymal stem cells with both the nanobarcode microbead system and the Affymetrix GeneChip ® HTA system, which is currently considered as the industrial standard. Though using only 1/20 of the sample RNA, the nanobarcode system showed sensitivity equivalent to Affymetrix GeneChip ® system. The coefficient of variation, dynamic range, and accuracy of the nanobarcodes measurement is equivalent to that of the GeneChip ® HTA system. Therefore, this newly invented nanobarcode microbead platform is thought to be sensitive, flexible, cost-effective and accurate in a level equivalent to the conventional methods. As an extension of the use of this new platform, spacecrafts may carry this miniaturized system as a diagnostic tool for the astronauts.

21 CFR 866.5640 - Infectious mononucleosis immunological test system.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Infectious mononucleosis immunological test system....5640 Infectious mononucleosis immunological test system. (a) Identification. An infectious mononucleosis immunological test system is a device that consists of the reagents used to measure by...

A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana.

Science.gov (United States)

Feng, Yue; Cao, Cong-Mei; Vikram, Meenu; Park, Sunghun; Kim, Hye Jin; Hong, Jong Chan; Cisneros-Zevallos, Luis; Koiwa, Hisashi

2011-03-08

Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.

A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana.

Directory of Open Access Journals (Sweden)

Yue Feng

Full Text Available Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A promoter, CBF3 (C-repeat Binding Factor 3 transcription factor and cpl1-2 (CTD phosphatase-like 1 mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1 transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.

Testing of modular industrial solar retrofit industrial process steam systems

Energy Technology Data Exchange (ETDEWEB)

Cameron, C.P.; Dudley, V.E.

1984-06-13

Under the Department of Energy's Modular Industrial Solar Retrofit project, five industrial process heat systems incorporating line-focus solar collectors were designed and hardware was installed and tested at Sandia National Laboratories and the Solar Energy Research Institute. System designers and collector manufacturers participating in the project included Acurex Solar Corporation, BDM, Inc., Custom Engineering, Inc., Foster Wheeler Solar Development Corporation, Solar Kinetics, Inc., and Suntec Systems, Inc. This paper describes the testing of the qualification test systems which has been under way since mid-1982. Each qualification test system includes an equipment skid sufficient to support a collector field of 2300 m/sup 2/ aperture and one delta-tempeature string of from 175 to 460 m/sup 2/ aperture. Each system is capable of producing saturated steam at 1.7 MPa and operates at maximum outlet temperatures of from 250 to 290/sup 0/C. The test series includes function and safety tests to determine that the systems operate as specified, an unattended operation test of at least two weeks duration, performance tests to allow prediction of annual system performance, and life cycle tests to evaluate component lifetime and maintenance requirements. Since the start of testing, some twenty five modifications have been made to the various systems for the purpose of improving system performance and/or reliability, and appropriate tests of these modifictions have been made or are underway. This paper presents a description of the approach to testing of the MISR systems and selected test results.

Diversity and testing requirements of programmable automation systems

International Nuclear Information System (INIS)

Haapanen, P.; Maskuniitty, M.

1993-04-01

In the report programmable digital operation and safety automation systems for nuclear power plants are discussed. The programmable systems deviate by their properties and behaviour from the conventional non-programmable systems in such extent, that their verification and validation for safety critical applications requires new methods and practices. The safety assessment can not be based on conventional probabilistic methods due to the difficulties in the quantification of the reliability of the software and hardware. A safety critical programmable system shall include diverse redundant parts so that no residual program fault can not cause the failure of the intended function of the system. Although complete testing of a programmable system is impossible, different tests have a central role in the production and validation process of the system. Diversity is important also in confidence building measures for the implemented system. Independent analysis and testing of the system should use different methods and tools from those used in the production and validation process by the system vendor. Use of diversity and testing are concluded to be central issues in producing safe programmable system and in proving them to be safe enough. By combining functional and programming diversity in a suitable way one can produce a system that is safe enough without having a non-programmable back-up system. (48 refs., 10 figs., 2 tabs.)

Challenges in testing genetically modified crops for potential increases in endogenous allergen expression for safety.

Science.gov (United States)

Panda, R; Ariyarathna, H; Amnuaycheewa, P; Tetteh, A; Pramod, S N; Taylor, S L; Ballmer-Weber, B K; Goodman, R E

2013-02-01

Premarket, genetically modified (GM) plants are assessed for potential risks of food allergy. The major risk would be transfer of a gene encoding an allergen or protein nearly identical to an allergen into a different food source, which can be assessed by specific serum testing. The potential that a newly expressed protein might become an allergen is evaluated based on resistance to digestion in pepsin and abundance in food fractions. If the modified plant is a common allergenic source (e.g. soybean), regulatory guidelines suggest testing for increases in the expression of endogenous allergens. Some regulators request evaluating endogenous allergens for rarely allergenic plants (e.g. maize and rice). Since allergic individuals must avoid foods containing their allergen (e.g. peanut, soybean, maize, or rice), the relevance of the tests is unclear. Furthermore, no acceptance criteria are established and little is known about the natural variation in allergen concentrations in these crops. Our results demonstrate a 15-fold difference in the major maize allergen, lipid transfer protein between nine varieties, and complex variation in IgE binding to various soybean varieties. We question the value of evaluating endogenous allergens in GM plants unless the intent of the modification was production of a hypoallergenic crop. © 2012 John Wiley & Sons A/S.

Spaceport Command and Control System User Interface Testing

Science.gov (United States)

Huesman, Jacob

2016-01-01

The Spaceport Command and Control System will be the National Aeronautics and Space Administration's newest system for launching commercial and government owned spacecraft. It's a large system with many parts all in need of testing. To improve upon testing already done by NASA engineers, the Engineering Directorate, Electrical Division (NE-E) of Kennedy Space Center has hired a group of interns each of the last few semesters to develop novel ways of improving the testing process.

Evaluation of the 3M™ Petrifilm™ Salmonella express system for the detection of Salmonella species in selected foods: collaborative study.

Science.gov (United States)

Bird, Patrick; Flannery, Jonathan; Crowley, Erin; Agin, James; Goins, David; Jechorek, Robert

2014-01-01

The 3M™ Petriflm™ Salmonella Express (SALX) System is a simple, ready-to-use chromogenic culture medium system for the rapid qualitative detection and biochemical confirmation of Salmonella spp. in food and food process environmental samples. The 3M Petrifilm SALX System was compared using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) Microbiology Laboratory Guidebook (MLG) 4.07 (2013) Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products and Carcass and Environmental Sponges for raw ground beef and the U.S. Food and Drug Administration Bacteriological Analytical Manual (FDA/BAM) Chapter 5, Salmonella (2011) reference method for dry dog food following the current AOAC validation guidelines. For this study, a total of 17 laboratories located throughout the continental United States evaluated 1872 test portions. For the 3M Petrifilm SALX System, raw ground beef was analyzed using 25 g test portions, and dry dog food was analyzed using 375 g test portions. For the reference methods, 25 g test portions of each inatrix were analyzed. The two matrices were artificially contaminated with Salmonella at three inoculation levels: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2-2 CFU/test portion), and a high inoculum level (2-5 CFU/test portion). Each inoculation level was statistically analyzed using the probability of detection statistical model. For the raw ground beef and dry dog food test portions, no significant differences at the 95% confidence interval were observed in the number of positive samples detected by the 3M Petrifilm SALX System versus either the USDA/FSIS-MLG or FDA/BAM methods.

Testing of a Wireless Sensor System for Instrumented Thermal Protection Systems

Science.gov (United States)

Kummer, Allen T.; Weir, Erik D.; Morris, Trey J.; Friedenberger, Corey W.; Singh, Aseem; Capuro, Robert M.; Bilen, Sven G.; Fu, Johnny; Swanson, Gregory T.; Hash, David B.

2011-01-01

Funded by NASA's Constellation Universities Institutes Project (CUIP), we have been developing and testing a system to wirelessly power and collect data from sensors on space platforms in general and, in particular, the harsh environment of spacecraft re-entry. The elimination of wires and associated failures such as chafing, sparking, ageing, and connector issues can increase reliability and design flexibility while reducing costs. These factors present an appealing case for the pursuit of wireless solutions for harsh environments, particularly for their use in space and on spacecraft. We have designed and built a prototype wireless sensor system. The system, with capabilities similar to that of a wired sensor system, was tested in NASA Ames Research Center s Aerodynamic Heating Facility and Interaction Heating Facility. This paper discusses the overall development effort, testing results, as well as future directions.

Tritium Systems Test Facility. Volume I

International Nuclear Information System (INIS)