Single step purification of recombinant proteins using the metal ion-inducible autocleavage (MIIA) domain as linker for tag removal.

Science.gov (United States)

Ibe, Susan; Schirrmeister, Jana; Zehner, Susanne

2015-08-20

For fast and easy purification, proteins are typically fused with an affinity tag, which often needs to be removed after purification. Here, we present a method for the removal of the affinity tag from the target protein in a single step protocol. The protein VIC_001052 of the coral pathogen Vibrio coralliilyticus ATCC BAA-450 contains a metal ion-inducible autocatalytic cleavage (MIIA) domain. Its coding sequence was inserted into an expression vector for the production of recombinant fusion proteins. Following, the target proteins MalE and mCherry were produced as MIIA-Strep fusion proteins in Escherichia coli. The target proteins could be separated from the MIIA-Strep part simply by the addition of calcium or manganese(II) ions within minutes. The cleavage is not affected in the pH range from 5.0 to 9.0 or at low temperatures (6°C). Autocleavage was also observed with immobilized protein on an affinity column. The protein yield was similar to that achieved with a conventional purification protocol. Copyright © 2015 Elsevier B.V. All rights reserved.

Uue õppekava mõju loodusainete õpetamisele / Aigi Kikkas, Miia Rannikmäe

Index Scriptorium Estoniae

Kikkas, Aigi

2000-01-01

1997/98 ja 1998/99 õa filmiti Tartu ja Tallinna koolides keemia-, bioloogia- ja füüsikatunde. Enne tunni algust toimus intervjuu õpetajatega, kus täpsustati tunni eesmärgid, teema, struktuur, kasutatavad meetodid ja õppematerjalid. Salvestatud õppetunde analüüsiti kolmes valdkonnas : tunni eesmärkide püstitamine ja nende realiseerimine, õpilaskesksuse kujundamine tunnis, tunni seotus igapäevaeluga

LoTe projektist saavad kasu nii koolid kui ka ülikoolid / Miia Rannikmäe

Index Scriptorium Estoniae

Rannikmäe, Miia, 1951-

2008-01-01

Tartu Ülikooli, Tallinna Ülikooli, Tallinna Tehnikaülikooli ja loodusainete õpetajaid ühendav projekt LoTe tähendab loodusteadusliku ja tehnoloogiaalase hariduse jätkusuutlikkuse tagamist kõigil õppetasanditel

Thermal unfolding of a Ca- and Lanthanide-binding protein

Energy Technology Data Exchange (ETDEWEB)

Fahmy, Karim [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Biophysics; Goettfert, M. [Technische Univ. Dresden (Germany); Knoeppel, J.

2017-06-01

The MIIA (metal ion-induced autocleavage)-domain of the protein Vic001052 from the pathogen Vibrio coralliilyticus, comprises 173 amino acids and exhibits Ca-dependent autoproteolytic activity. It shows homology to nodulation proteins which are secreted by Rhizobiacea into plant host cells where they exert Ca-dependent functions. We have studied the structural and energetic aspects of metal protein interactions of the MIIA domain which appear attractive for engineering metal-binding synthetic peptides. Using a non-cleavable MIIA domain construct, we detected very similar structural changes upon binding to Ca{sup 2+} and Eu{sup 3+}. The thermal denaturation of the Ca-bound state was studied by circular dichroism spectroscopy. The metal-bound folded state unfolds reversibly into an unstructured metal-free state similar to the metal-free state at room temperature.

Lastemuuseum Miia-Milla-Manda = The Miia-Milla-Manda Children's Museum / Margit Mutso

Index Scriptorium Estoniae

Mutso, Margit, 1966-

2010-01-01

Tallinnas Koidula 21A asuva hoone restaureerimisest, lastemuuseumi sisekujundusest. Kadrioru Lastepargi paviljoni (arhitekt Villem Seidra, 1937) renoveerimisprojekti tegi arhitekt Katrin Etverk, sisearhitektid ja ekspositsiooni kujundus: Maile Grünberg, Eliina Grünberg (Grünberg oü). Ajaloolane Aleksandr Pantelejev. Žürii liikme Mait Summataveti hinnang kultuurkapitali aastapreemiale esitatud hoonele

Erkki Huttuneni arhitektuuriteos Liivirannas / Andri Ksenofontov

Index Scriptorium Estoniae

Ksenofontov, Andri, 1962-

1996-01-01

Liivlaste Rahvamaja liivlaste keskuses Ire's (läti k. Mazirbe). Projektide rahvusvahelise konkursi võitis soome arhitekt Erkki Huttunen (1901-1956). Lõpliku projekti koostas võistlustöö põhjal läti arhitekt Visvaldis Paegle. Avati 5. aug. 1939. Hoone arhitektuurist. Arvamusi Teppo Jokinenilt.

"Andmebaasid teenivad kodanike huve" / Mati Rahu

Index Scriptorium Estoniae

Rahu, Mati, 1942-

1998-01-01

Meditsiiniregistritest ja delikaatsete isikuandmete kaitsest. Vestlus Eksperimentaalse ja Kliinilise meditsiini epidemioloogia ja biostatistika osakonna juhatajaga. Vt. ka küsitlus: Mis juhtuks, kui Teie riigi või uurimisvaldkonna meditsiiniregistritest eemaldataks kõik isikuandmed?, lk. 15-16: M. Mckee, J.L. Young jr., L. Teppo, M. Parkin, H.H. Storm

Saates armastuse sõnumeid / Aita Kivi

Index Scriptorium Estoniae

Kivi, Aita, 1954-

2004-01-01

Filoloogi Mai-Liis Napa ning juristi ja kirjaniku Martin Napa perekonnast. Pereliikmete ühiseks harrastuseks on maalimine. Elatakse Lillekülas 1939. a. ehitatud majas, mis on ehitatud suuremaks ja mugavamaks. Ka Miia Masso projekteeritud talveaiast. 8 ill

The (Proper) Microfoundations of Routines and Capabilities

DEFF Research Database (Denmark)

Felin, Teppo; Foss, Nicolai Juul

2012-01-01

Sidney Winter (2011), Brian Pentland (2011), and Geoffrey Hodgson and Thorbjørn Knudsen (2011) take issue with the arguments in Teppo Felin and Nicolai J. Foss (2011), along with more generally critiquing the ‘microfoundations project’ related to routines and capabilities. In this rejoinder we ar...... chauvinism; (3) models of mind and man; (4) levels of analysis; (5) agency and uncaused causes; and then further discuss (6) a rationalist alternative....

Maaülikooli uksed uutele tulijatele valla / Sirje Pärismaa

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Pärismaa, Sirje, 1958-

2008-01-01

Oma õpingutest räägivad maaülikooli tudengid: Kadi Avi (majandusarvestus ja finantsjuhtimine III), Kaiti Pilk (maastikuarhitekt III), Mart Kelk (metsatööstuse magistrantuur I), Karin Ojamäe (rakendushüdrobioloogia III), Heidi Heitur (ökonoomika ja ettevõtlus I); Margus Paesalu (metsatööstus III), Kadri Kalle ( maastikukaitse ja -hoolduse magistrantuur I), Liisi Õunapuu (loodusvarade kasutamine ja kaitse III), Laura Niemelä (veterinaarmeditsiin IV) ja Peep Teppo (metsamajanduse magistrantuur II) ning õppeprorektor Jüri Lehtsaar

A Real-Time Systems Symposium Preprint.

Science.gov (United States)

1983-09-01

Real - Time Systems Symposium Preprint Interim Tech...estimate of the occurence of the error. Unclassii ledSECUqITY CLASSIF’ICA T" NO MI*IA If’ inDI /’rrd erter for~~ble. ’Corrputnqg A REAL - TIME SYSTEMS SYMPOSIUM...ABSTRACT This technical report contains a preprint of a paper accepted for presentation at the REAL - TIME SYSTEMS SYMPOSIUM, Arlington,

A road map for wireless mesh routing with DSA

CSIR Research Space (South Africa)

Maliwatu, R

2016-09-01

Full Text Available of TV spectrum: The controversy at the Federal Communications Commission, IEEE Communications Magazine, 43(5):24–25, 2005. [2] Marja Matinmikko, Miia Mustonen, Dennis Roberson, et al. Overview and comparison of recent spectrum sharing approaches... in regulation and research: From opportunistic unlicensed access towards licensed shared access, In 2014 IEEE International Symposium on Dynamic Spectrum Access Networks, DYSPAN 2014, pages 92–102, McLean, VA, 2014. [3] M. Nekovee. Current Trends in Regulation...

Effects of PPARs agonists on cardiac metabolism in littermate and cardiomyocyte-specific PPAR-γ-knockout (CM-PGKO mice.

Directory of Open Access Journals (Sweden)

Michelangela Barbieri

Full Text Available Understanding the molecular regulatory mechanisms controlling for myocardial lipid metabolism is of critical importance for the development of new therapeutic strategies for heart diseases. The role of PPARγ and thiazolidinediones in regulation of myocardial lipid metabolism is controversial. The aim of our study was to assess the role of PPARγ on myocardial lipid metabolism and function and differentiate local/from systemic actions of PPARs agonists using cardiomyocyte-specific PPARγ -knockout (CM-PGKO mice. To this aim, the effect of PPARγ, PPARγ/PPARα and PPARα agonists on cardiac function, intra-myocyte lipid accumulation and myocardial expression profile of genes and proteins, affecting lipid oxidation, uptake, synthesis, and storage (CD36, CPT1MIIA, AOX, FAS, SREBP1-c and ADPR was evaluated in cardiomyocyte-specific PPARγ-knockout (CM-PGKO and littermate control mice undergoing standard and high fat diet (HFD. At baseline, protein levels and mRNA expression of genes involved in lipid uptake, oxidation, synthesis, and accumulation of CM-PGKO mice were not significantly different from those of their littermate controls. At baseline, no difference in myocardial lipid content was found between CM-PGKO and littermate controls. In standard condition, pioglitazone and rosiglitazone do not affect myocardial metabolism while, fenofibrate treatment significantly increased CD36 and CPT1MIIA gene expression. In both CM-PGKO and control mice submitted to HFD, six weeks of treatment with rosiglitazone, fenofibrate and pioglitazone lowered myocardial lipid accumulation shifting myocardial substrate utilization towards greater contribution of glucose. In conclusion, at baseline, PPARγ does not play a crucial role in regulating cardiac metabolism in mice, probably due to its low myocardial expression. PPARs agonists, indirectly protect myocardium from lipotoxic damage likely reducing fatty acids delivery to the heart through the actions on adipose

Orthognathic Surgery in Craniofacial Microsomia: Treatment Algorithm

Science.gov (United States)

Valladares, Salvador; Torrealba, Ramón; Nuñez, Marcelo; Uribe, Francisca

2015-01-01

Summary: Craniofacial microsomia is a broad term that covers a variety of craniofacial malformation conditions that are caused by alterations in the derivatives of the first and second pharyngeal arches. In general terms, diverse therapeutic alternatives are proposed according to the growth stage and the severity of the alteration. When craniofacial growth has concluded, conventional orthognathic surgery (Le Fort I osteotomy, bilateral sagittal split osteotomy, and genioplasty) provides good alternatives for MI and MIIA type cases. Reconstruction of the mandibular ramus and temporomandibular joint before orthognathic surgery is the indicated treatment for cases MIIB and MIII. The goal of this article is to establish a surgical treatment algorithm for orthognathic surgery on patients with craniofacial microsomia, analyzing the points that allow the ideal treatment for each patient to be chosen. PMID:25674375

Linnulennult 2008. aasta lastekirjandusest / Mare Müürsepp

Index Scriptorium Estoniae

Müürsepp, Mare, 1958-

2009-01-01

Lühidalt on iseloomustatud järgmisi 2008. a. ilmunud lasteraamatuid: Raud, Piret. Printsess Luluu ja härra Kere (Tänapäev) ; Vilep, Heiki. Horlok ja Ürgvärava võti (A-Disain) ; Reinaus, Reeli. Saladuslik päevik (Tänapäev) ; Kumberg, Krista. Autopõnn Anto läheb seiklema (Koolibri) ; Ausman, Piia. Kaptenid avastavad maailma (Haus Galerii) ; Selli, Tiia. Neletriin ja Kapi-Kaarup (Tänapäev) ; Samuel, Evelin. Ükskord, kui sadas vihma (Ajakirjade Kirjastus) ; Pervik, Aino. Presidendilood (Tänapäev) ; Vainola, Kätlin. Mia, Konrad ja avanevad uksed (Tänapäev) ; Smitt, Katri. Varesejalakiri (Ilo) ; Käo, Henno. Mina, emme ja teised (Tänapäev) ; Saarna, Meelike. Mattias ja mamma (Tänapäev) ; Hinrikus, Kadri. Miia ja Friida (Eesti Ekspressi Kirjastus) ; Soopan, Ivar. Kõik poisid ei saa suureks (Eesti Päevaleht) ; Kutsar, Kuulo. Mõmmikute telerihaigus (Ilo)

Lastekirjandus 2008 kurja kriitiku pilgu läbi / Ilona Martson

Index Scriptorium Estoniae

Martson, Ilona, 1970-

2009-01-01

Ülevaade 2008. aasta eesti lastekirjandusest. Pikemalt käsitletakse järgmisi autoreid ja nende teoseid: Piret Raud "Printsess Luluu", Aino Pervik "Presidendilood", Kristiina Kass "Petra lood", Kätlin Vainola "Mia, Konrad ja avanevad uksed", Kerttu Soans "Külaline vannis", Wimberg "Härra Padakonn", Leelo Tungal "Seltsimees laps", Kadri Hinrikus "Miia ja Friida", Ivar Soopan "Kõik poisid ei saa suureks", Siiri Laidla "Raamatukogunõid Rosaalie", Siiri Laidla "Armas hunt", Reeli Reinaus "Saladuslik päevik", Tiia Selli "Neletriin ja Kapi-Kaarup", Markus Saksatamm "Võlur Sinku-Vinku ja nõiatrall", Evelin Samuel "Ükskord, kui sadas vihma", Mikä Keränen "Varastatud oranž jalgratas", Ivo Parbus "Draakon Rudolf", Urmas Nemvalts "Väikeste meeste lennumasinajutud", Lehte Hainsalu "Väike valge", Jaan Tangsoo "Mina ja minu pere", Aidi Vallik "Pints ja Tutsik", Heiki Vilep "Horlok ja Ürgvärava võti", Evelin Samuel "Hiigelsoojad pilvelambad", Sulev Oll "Hea tuju kuju", Paul-Eerik Rummo "Kui lähed teele koidu eel", Heljo Mänd "Suve suurmüük", Heljo Mänd "Sügise süda", Heljo Mänd "Kevade reklaam", Heljo Mänd "Talve Top", Ülo Pikkov. Presidendi torukübar

Comparison between actual and predicted postoperative stair-climbing test, walk test and spirometric values in patients undergoing lung resection Comparação dos testes de escada, caminhada e espirometria preditos com os obtidos no pós-operatório de ressecções pulmonares

Directory of Open Access Journals (Sweden)

Marcos Vinícius Cataneo Pancieri

2010-12-01

cada teste. Estatística: foi utilizada a análise de variância para medidas repetidas (ANOVA, com significância de 5%. RESULTADOS: Foram estudados 40 pacientes. A ressecção pulmonar variou desde o ganho de dois segmentos funcionantes até a perda de 9. Os valores pré, ppo e pós foram respectivamente: VEF1 -pré = 2,6±0,8L, ppo = 2,3±0,8L, pós = 2,3±0,8L (VEF1pré > VEF1ppo = VEF1pós, TC6-pré = 604±63m, ppo = 529±103m, pós = 599±74m (TC6pré = TC6pós > TC6ppo, tTE-pré = 32,9±7,6s, ppo = 37,8±12,1s, pós = 33,7±8,5s (tTEpré = tTEpós < tTEppo. CONCLUSÃO: Nas ressecções pulmonares, este grupo de pacientes perdeu função pulmonar medida através da espirometria, mas não perdeu a capacidade de exercício, medida através dos testes de escada e caminhada.

EDITORIAL The 17th Central European Workshop on Quantum Optics

Science.gov (United States)

Man'ko, Margarita A.

2011-02-01

), held at the University of Turku, are also available (2010 Phys. Scr. T140). The 18th Central European Workshop on Quantum Optics (CEWQO11) will be held in Madrid, Spain on 30 May--3 June 2011. It will be chaired by Professor Luis Lorenzo Sanchez Soto from the Complutense University of Madrid. List of Papers The 17th Central European Workshop on Quantum Optics in St Andrews, Scotland Ulf Leonhardt and Natalia Korolkova Double self-Kerr scheme for optical Schrödinger-cat state preparation P Adam, Z Darázs, T Kiss and M Mechler Relations between scaling transformed Husimi functions, Wigner functions and symplectic tomograms describing corresponding physical states V A Andreev, D M Davidović, L D Davidović and M D Davidović Entanglement dynamics of two independent cavity-embedded quantum dots B Bellomo, G Compagno, R Lo Franco, A Ridolfo and S Savasta Dynamical stabilization of spin systems in time-dependent magnetic fields Yu V Bezvershenko, P I Holod and A Messina Entanglement dynamics of a bipartite system in squeezed vacuum reservoirs Smail Bougouffa and Awatif Hindi On Wheeler's delayed-choice Gedankenexperiment and its laboratory realization M Božić, L Vušković, M Davidović and Á S Sanz A smooth, holographically generated ring trap for the investigation of superfluidity in ultracold atoms Graham D Bruce, James Mayoh, Giuseppe Smirne, Lara Torralbo-Campo and Donatella Cassettari Parametric amplification of the classical field in cavities with photoexcited semiconductors V V Dodonov Mutually unbiased bases: tomography of spin states and the star-product scheme S N Filippov and V I Man'ko Quantum trajectory model for photon detectors and optoelectronic devices Teppo Häyrynen, Jani Oksanen and Jukka Tulkki Entanglement in two-mode continuous variable open quantum systems Aurelian Isar A classical field comeback? The classical field viewpoint on triparticle entanglement Andrei Khrennikov Experimental investigation of the enhancement factor and the cross