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Sample records for tein eno tnisson

  1. Tantsufilm "Teine" ETV-s

    Index Scriptorium Estoniae

    2005-01-01

    Täna on ETV-s rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre : stsenaristid Rene Vilbre, Teet Kask, Helena Jonsdottir : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir : osatäitjad Mait Malmsten, Ruslan Stepanov. Filmi võtted toimusid Tartu vanglas

  2. "Teine" võitis Prantsusmaal Grand Prix'

    Index Scriptorium Estoniae

    2006-01-01

    Prantsusmaal Essonne'is toimuval 8. Euroopa filmifestivalil Cinessonne sai üliõpilaste žürii grand prix rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre. Ka teistest festivalidest, kus film osalenud

  3. Parem teine kui viimane / Andreas Kaju

    Index Scriptorium Estoniae

    Kaju, Andreas

    2006-01-01

    Internet peaks olema kõikidele Eesti inimestele tagatud põhiõigus. Sama ka Võrumaa Teataja 25. mai 2006, lk. 2 ; Põhjarannik 25. mai 2006, lk. 2 ; Koit 25. mai 2006, lk. 6 ; Meie Maa 25. mai 2006, lk. 2 ; Sakala 26. mai lk. 2 ; Vooremaa 30. mai 2006, lk. 2 ; Hiiu Leht 30. mai 2006, lk. 2 ; Elva Postipoiss 3. juuni 2006, lk. 4 : Lääne Elu 30. mai 2006, lk. 2, pealkiri kujul : Tasuta internet etrvele riigile - parem teine kui viimane

  4. Tagasiviitavad tõendid: teine pilk pilditeadusele / Beat Wyss ; intervjueerinud Ivar-Kristjan Hein

    Index Scriptorium Estoniae

    Wyss, Beat, 1947-

    2011-01-01

    Intervjuus Karlsruhe kunsti- ja disainiülikooli kunstiajaloo ja meediateooria professori Beat Wyssiga on teemaks Zürichis tema juhitav uurimisprojekt, mille keskmes on Venezia biennaal Ida-Euroopa riikide positsioonilt, tema EKA Kunstiteaduse Instituudi doktorantidele peetud seminar "Tagasiviitavad tõendid. Teine pilk pilditeadusele", tema kaasaegse kunsti universaalsed põhimõtted

  5. "Teine" ja teistmoodi, eriline ja erinev muuseumis = The "other" : the unique and the different in museums / Adele-Marie Dzidzaria

    Index Scriptorium Estoniae

    Dzidzaria, Adele-Marie

    2006-01-01

    "Kambrist" muuseumiks, privaatsest avalikuks. Kultuuriline "teine" - kolooniad, immigrandid ja integratsioon. Ühiskondlikud "teised" - hullud ja vangid. "Läbipaistva" muuseumi idee ja "teistsugused" ekspositsioonid. A.-M. Dzidzaria - Amsterdami Reinwardti Akadeemia rahvusvaheline museoloogia magister

  6. The microstructure and composition of equilibrium phases formed in hypoeutectic Te-In alloy during solidification

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Baoguang [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, Beijing 100083 (China); Hu, Jinwu [Center of Failure Analysis, Central Iron and Steel Research Institute, Beijing 100081 (China); Wang, Chongyun; Yang, Wenhui [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, Beijing 100083 (China); Tian, Wenhuai, E-mail: wenhuaitian@ustb.edu.cn [Department of Materials Physics and Chemistry, University of Science and Technology Beijing, Beijing 100083 (China)

    2017-03-15

    As a key tellurium atoms evaporation source for ultraviolet detection photocathode, the hypoeutectic Te{sub 75}In{sub 25} alloy was prepared by employing a slow solidification speed of about 10{sup −2} K/s. The microstructure and chemical composition of the equilibrium phases formed in the as-prepared alloy were studied in this research work. The experimental results show that the as-prepared Te-In alloy was constituted by primary In{sub 2}Te{sub 5} phase and eutectic In{sub 2}Te{sub 5}/Te phases. The eutectic In{sub 2}Te{sub 5}/Te phases are distributed in the grain boundaries of primary In{sub 2}Te{sub 5} phase. With the slow solidification speed, a pure eutectic Te phase without any excessive indium solute was obtained, where Te content of eutectic Te phase is 100 mass%. Moreover, it can be considered that the stress between the In{sub 2}Te{sub 5} and Te phases plays an important role in reducing the tellurium vapor pressure in Te{sub 75}In{sub 25} alloy. - Highlights: • The microstructure of Te-In alloy as an evaporation source was analyzed. • A pure eutectic Te phase was obtained by using a slow solidification speed method. • The relation between vapor pressure and inner-stress in the alloy was discussed.

  7. The flyby of Rosetta at asteroid Šteins - mission and science operations

    Science.gov (United States)

    Accomazzo, Andrea; Wirth, Kristin R.; Lodiot, Sylvain; Küppers, Michael; Schwehm, Gerhard

    2010-07-01

    The international Rosetta mission, a cornerstone mission of the european space agency scientific Programme, was launched on 2nd March 2004 on its 10 years journey towards a rendezvous with comet Churyumov-Gerasimenko ( Gardini et al., 1999). During its interplanetary flight towards its target Rosetta crosses the asteroid belt twice with the opportunity to observe at close quarters two asteroids: (2867)-Šteins in 2008 and (21)-Lutetia in 2010. The spacecraft design was such that these opportunities could be fully exploited to deliver valuable data to the scientific community. The mission trajectory was controlled such that Rosetta would fly next to asteroid Šteins on the 5th of September 2008 with a relative speed of 8.6 km/s at a minimum distance of 800 km. Mission operations have been carefully planned to achieve the best possible flyby scenario and scientific outcome. The flyby scenario, the optical navigation campaign, and the planning of the scientific observations had to be adapted by the Mission and the Science Operations Centres to the demanding requirements expressed by the scientific community. The flyby was conducted as planned with a large number of successful observations.

  8. Low shear stress induces vascular eNOS uncoupling via autophagy-mediated eNOS phosphorylation.

    Science.gov (United States)

    Zhang, Jun-Xia; Qu, Xin-Liang; Chu, Peng; Xie, Du-Jiang; Zhu, Lin-Lin; Chao, Yue-Lin; Li, Li; Zhang, Jun-Jie; Chen, Shao-Liang

    2018-05-01

    Uncoupled endothelial nitric oxide synthase (eNOS) produces O 2 - instead of nitric oxide (NO). Earlier, we reported rapamycin, an autophagy inducer and inhibitor of cellular proliferation, attenuated low shear stress (SS) induced O 2 - production. Nevertheless, it is unclear whether autophagy plays a critical role in the regulation of eNOS uncoupling. Therefore, this study aimed to investigate the modulation of autophagy on eNOS uncoupling induced by low SS exposure. We found that low SS induced endothelial O 2 - burst, which was accompanied by reduced NO release. Furthermore, inhibition of eNOS by L-NAME conspicuously attenuated low SS-induced O 2 - releasing, indicating eNOS uncoupling. Autophagy markers such as LC3 II/I ratio, amount of Beclin1, as well as ULK1/Atg1 were increased during low SS exposure, whereas autophagic degradation of p62/SQSTM1 was markedly reduced, implying impaired autophagic flux. Interestingly, low SS-induced NO reduction could be reversed by rapamycin, WYE-354 or ATG5 overexpression vector via restoration of autophagic flux, but not by N-acetylcysteine or apocynin. eNOS uncoupling might be ascribed to autophagic flux blockade because phosphorylation of eNOS Thr495 by low SS or PMA stimulation was also regulated by autophagy. In contrast, eNOS acetylation was not found to be regulated by low SS and autophagy. Notably, although low SS had no influence on eNOS Ser1177 phosphorylation, whereas boosted eNOS Ser1177 phosphorylation by rapamycin were in favor of the eNOS recoupling through restoration of autophagic flux. Taken together, we reported a novel mechanism for regulation of eNOS uncoupling by low SS via autophagy-mediated eNOS phosphorylation, which is implicated in geometrical nature of atherogenesis. Copyright © 2018 Elsevier B.V. All rights reserved.

  9. Brian Eno Hollandi Festivali peakülaliseks

    Index Scriptorium Estoniae

    1999-01-01

    Inglise helilooja, videokunstnik ja muusikaprodutsent Brian Eno on 5.-26. juunini Amsterdamis toimuva avangardse Hollandi Festivali peakülaline, kelle videoinstallatsioon seatakse üles Stedelijki Muuseumis. Festivali programmist

  10. Vascular nitric oxide: Beyond eNOS

    Directory of Open Access Journals (Sweden)

    Yingzi Zhao

    2015-10-01

    Full Text Available As the first discovered gaseous signaling molecule, nitric oxide (NO affects a number of cellular processes, including those involving vascular cells. This brief review summarizes the contribution of NO to the regulation of vascular tone and its sources in the blood vessel wall. NO regulates the degree of contraction of vascular smooth muscle cells mainly by stimulating soluble guanylyl cyclase (sGC to produce cyclic guanosine monophosphate (cGMP, although cGMP-independent signaling [S-nitrosylation of target proteins, activation of sarco/endoplasmic reticulum calcium ATPase (SERCA or production of cyclic inosine monophosphate (cIMP] also can be involved. In the blood vessel wall, NO is produced mainly from l-arginine by the enzyme endothelial nitric oxide synthase (eNOS but it can also be released non-enzymatically from S-nitrosothiols or from nitrate/nitrite. Dysfunction in the production and/or the bioavailability of NO characterizes endothelial dysfunction, which is associated with cardiovascular diseases such as hypertension and atherosclerosis.

  11. Crataegus Special Extract WS 1442 Effects on eNOS and microRNA 155.

    Science.gov (United States)

    Wang, Xinwen; Liang, Yan; Shi, Jian; Zhu, Hao-Jie; Bleske, Barry E

    2018-04-16

    Increased expression of microRNA 155 (miR-155) results in a decrease in endothelial nitric oxide synthase (eNOS) expression and impaired endothelial function. Factors that have been shown to increase expression of miR-155 may be mitigated by WS 1442, an extract of hawthorn leaves and flowers ( Crataegus special extract) that contains a range of pharmacologically active substances including oligomeric proanthocyanidins and flavonoids. The purpose of this study is to determine the effect of WS 1442 on the expression of miR-155 and eNOS in the presence of tumor necrosis factor (TNF- α ). Human umbilical vein endothelial cells (HUVECs) were studied after the exposure to TNF- α , with or without simvastatin (positive control) and WS 1442. The expression levels of eNOS, phosphorylated eNOS, and miR-155 in the different HUVEC treatment groups were determined by western blot and quantitative real-time polymerase chain reaction, respectively. To evaluate the effect of WS 1442 on the eNOS activity, the medium and intracellular nitrate/nitrite (NO) concentrations were also analyzed using a colorimetric Griess assay kit. The results demonstrated that TNF- α upregulated miR-155 expression and decreased eNOS expression and NO concentrations. WS 1442 also increased miR-155 expression and decreased eNOS expression but, unlike TNF- α , increased phosphorylated eNOS expression and NO concentrations. Surprisingly, WS 1442 increased miR-155 expression; however, WS 1442 mitigated the overall negative effect of miR-155 on decreasing eNOS expression by increasing expression of phosphorylated eNOS and resulting in an increase in NO concentrations. In the setting where miR-155 may be expressed, WS 1442 may offer vascular protection by increasing the expression of phosphorylated eNOS. Georg Thieme Verlag KG Stuttgart · New York.

  12. Association between nucleotide mutation of eNOS gene and serum ...

    African Journals Online (AJOL)

    Various mutation on endothelial nitric oxide synthase (eNOs) gene cause reduced production of NO, the expansion factor (VEF) and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C polymorphism of the gene or nucleotide mutation of eNOS gene in patients ...

  13. Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death

    International Nuclear Information System (INIS)

    Ejeskär, Katarina; Krona, Cecilia; Carén, Helena; Zaibak, Faten; Li, Lingli; Martinsson, Tommy; Ioannou, Panayiotis A

    2005-01-01

    Neuroblastoma is a solid tumour of childhood often with an unfavourable outcome. One common genetic feature in aggressive tumours is 1p-deletion. The α-enolase (ENO1) gene is located in chromosome region 1p36.2, within the common region of deletion in neuroblastoma. One alternative translated product of the ENO1 gene, known as MBP-1, acts as a negative regulator of the c-myc oncogene, making the ENO1 gene a candidate as a tumour suppressor gene. Methods used in this study are transfection of cDNA-vectors and in vitro transcribed mRNA, cell growth assay, TUNEL-assay, real-time RT-PCR (TaqMan) for expression studies, genomic sequencing and DHPLC for mutation detection. Here we demonstrate that transfection of ENO1 cDNA into 1p-deleted neuroblastoma cell lines causes' reduced number of viable cells over time compared to a negative control and that it induces apoptosis. Interestingly, a similar but much stronger dose-dependent reduction of cell growth was observed by transfection of in vitro transcribed ENO1 mRNA into neuroblastoma cells. These effects could also be shown in non-neuroblastoma cells (293-cells), indicating ENO1 to have general tumour suppressor activity. Expression of ENO1 is detectable in primary neuroblastomas of all different stages and no difference in the level of expression can be detected between 1p-deleted and 1p-intact tumour samples. Although small numbers (11 primary neuroblastomas), there is some evidence that Stage 4 tumours has a lower level of ENO1-mRNA than Stage 2 tumours (p = 0.01). However, mutation screening of 44 primary neuroblastomas of all different stages, failed to detect any mutations. Our studies indicate that ENO1 has tumour suppressor activity and that high level of ENO1 expression has growth inhibitory effects

  14. A central role of eNOS in the protective effect of wine against metabolic syndrome.

    Science.gov (United States)

    Leighton, Federico; Miranda-Rottmann, Soledad; Urquiaga, Inés

    2006-01-01

    The positive health effects derived from moderate wine consumption are pleiotropic. They appear as improvements in cardiovascular risk factors such as plasma lipids, haemostatic mechanisms, endothelial function and antioxidant defences. The active principles would be ethanol and mainly polyphenols. Results from our and other laboratories support the unifying hypothesis that the improvements in risk factors after red wine consumption are mediated by endothelial nitric oxide synthase (eNOS). Many genes are involved, but the participation of eNOS would be a constant feature. The metabolic syndrome is a cluster of metabolic risk factors associated with high risk of cardiovascular disease (CVD). The National Cholesterol Education Programmmes Adult Treatment Panel III (NCEPATP III) clinical definition of the metabolic syndrome requires the presence of at least three risk factors, from among abdominal obesity, high plasma triacylglycerols, low plasma HDL, high blood pressure and high fasting plasma glucose. The molecular mechanisms responsible for the metabolic syndrome are not known. Since metabolic syndrome apparently affects 10-30% of the population in the world, research on its pathogenesis and control is needed. The recent finding that eNOS knockout mice present a cluster of cardiovascular risk factors comparable to those of the metabolic syndrome suggests that defects in eNOS function may cause human metabolic syndrome. These mice are hypertensive, insulin resistant and dyslipidemic. Further support for a pathogenic role of eNOS comes from the finding in humans that eNOS polymorphisms associate with insulin resistance and diabetes, with hypertension, with inflammatory and oxidative stress markers and with albuminuria. So, the data sustain the hypothesis that eNOS enhancement should reduce metabolic syndrome incidence and its consequences. Therefore red wine, since it enhances eNOS function, should be considered as a potential tool for the control of metabolic

  15. Circulating Blood eNOS Contributes to the Regulation of Systemic Blood Pressure and Nitrite Homeostasis

    Science.gov (United States)

    Wood, Katherine C.; Cortese-Krott, Miriam M.; Kovacic, Jason C.; Noguchi, Audrey; Liu, Virginia B.; Wang, Xunde; Raghavachari, Nalini; Boehm, Manfred; Kato, Gregory J.; Kelm, Malte; Gladwin, Mark T.

    2013-01-01

    Objective Mice genetically deficient in endothelial nitric oxide synthase (eNOS−/−) are hypertensive with lower circulating nitrite levels, indicating the importance of constitutively produced nitric oxide (NO•) to blood pressure regulation and vascular homeostasis. While the current paradigm holds that this bioactivity derives specifically from expression of eNOS in endothelium, circulating blood cells also express eNOS protein. A functional red cell eNOS that modulates vascular NO• signaling has been proposed. Approach and Results To test the hypothesis that blood cells contribute to mammalian blood pressure regulation via eNOS-dependent NO• generation, we cross-transplanted WT and eNOS−/− mice, producing chimeras competent or deficient for eNOS expression in circulating blood cells. Surprisingly, we observed a significant contribution of both endothelial and circulating blood cell eNOS to blood pressure and systemic nitrite levels, the latter being a major component of the circulating NO• reservoir. These effects were abolished by the NOS inhibitor L-NAME and repristinated by the NOS substrate L-Arginine, and were independent of platelet or leukocyte depletion. Mouse erythrocytes were also found to carry an eNOS protein and convert 14C-Arginine into 14C-Citrulline in a NOS-dependent fashion. Conclusions These are the first studies to definitively establish a role for a blood borne eNOS, using cross transplant chimera models, that contributes to the regulation of blood pressure and nitrite homeostasis. This work provides evidence suggesting that erythrocyte eNOS may mediate this effect. PMID:23702660

  16. Activation of Endothelial Nitric Oxide (eNOS Occurs through Different Membrane Domains in Endothelial Cells.

    Directory of Open Access Journals (Sweden)

    Jason Tran

    Full Text Available Endothelial cells respond to a large range of stimuli including circulating lipoproteins, growth factors and changes in haemodynamic mechanical forces to regulate the activity of endothelial nitric oxide synthase (eNOS and maintain blood pressure. While many signalling pathways have been mapped, the identities of membrane domains through which these signals are transmitted are less well characterized. Here, we manipulated bovine aortic endothelial cells (BAEC with cholesterol and the oxysterol 7-ketocholesterol (7KC. Using a range of microscopy techniques including confocal, 2-photon, super-resolution and electron microscopy, we found that sterol enrichment had differential effects on eNOS and caveolin-1 (Cav1 colocalisation, membrane order of the plasma membrane, caveolae numbers and Cav1 clustering. We found a correlation between cholesterol-induced condensation of the plasma membrane and enhanced high density lipoprotein (HDL-induced eNOS activity and phosphorylation suggesting that cholesterol domains, but not individual caveolae, mediate HDL stimulation of eNOS. Vascular endothelial growth factor (VEGF-induced and shear stress-induced eNOS activity was relatively independent of membrane order and may be predominantly controlled by the number of caveolae on the cell surface. Taken together, our data suggest that signals that activate and phosphorylate eNOS are transmitted through distinct membrane domains in endothelial cells.

  17. Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

    Science.gov (United States)

    Cerqueira, Fernanda M; Laurindo, Francisco R M; Kowaltowski, Alicia J

    2011-03-31

    Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR). Since treatment of mice with dinitrophenol (DNP) promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.

  18. Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

    Directory of Open Access Journals (Sweden)

    Fernanda M Cerqueira

    2011-03-01

    Full Text Available Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR. Since treatment of mice with dinitrophenol (DNP promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.

  19. Tidal breath eNO measurements in a cohort of unsedated hospitalized neonates-A method validation

    DEFF Research Database (Denmark)

    Schmidt, Birgitte J; Reim, Pauline S; Jensen, Andreas K

    2018-01-01

    to validate clinically feasible longitudinal online tidal eNO and V'NO in a real-life birth cohort of un-sedated, hospitalized preterm, and term neonates. METHOD: We included 149 newborns, GA 28-42 weeks. Each scheduled for six repeated, non-invasive, on-line eNO measurements with Ecomedics CLD 88sp and NO...

  20. Perspectives in medical education 7. Observations on clinical training at a "US-style" residency program at Teine Keijinkai Hospital in Sapporo, Japan.

    Science.gov (United States)

    Rao, R Harsha

    2009-06-01

    The residency program at Teine Keijinkai Hospital in Sapporo has successfully implemented a training philosophy that is focused on the development of clinical skills and critical thinking in Japanese residents. Several elements contribute to its success. The first and foremost is visionary physician leadership, beginning with the pioneers who implemented the philosophy, and continuing through the current leadership, which has sustained the original vision. A close second is the administrative and financial commitment to invest in producing more clinically accomplished Japanese physicians, long before that need was officially recognized. Third is the program's explicit aim of adhering to international norms by requiring three years of training, promulgating a benevolent, not paternalistic teaching philosophy and encouraging an interactive and interrogatory learning ethic. Fourth is the year-round presence of a US-trained Physician-in-Residence, to sustain the focus on clinical skills and international norms. Fifth is a long-term relationship with the Internal Medicine Training Program at the University of Pittsburgh, providing a conduit for ongoing academic exchange and programmatic advice. Last, but not least, is its avowed intention of being viewed as an "American-style program" with a preference for English fluent applicants, which acts as a magnet for trainees motivated to acquire clinical skills and competencies, with an eye to future training in the US. All these elements contribute to the program's unique focus on teaching clinical skills and critical thinking. Others who are striving with varying degrees of success to implement a similar philosophy in Japan may benefit from studying its example.

  1. Orthodontic force stimulates eNOS and iNOS in rat osteocytes

    NARCIS (Netherlands)

    Tan, S.D.; Xie, R.; Klein Nulend, J.; van Rheden, R.E.; Bronckers, A.L.J.J.; Kuijpers-Jagtman, A.M.; Von den Hoff, J.W.; Maltha, J.C.

    2009-01-01

    Mechanosensitive osteocytes are essential for bone remodeling. Nitric oxide, an important regulator of bone remodeling, is produced by osteocytes through the activity of constitutive endothelial nitric oxide synthase (eNOS) or inducible nitric oxide synthase (iNOS). We hypothesized that these

  2. Orthodontic force stimulates eNOS and iNOS in rat osteocytes.

    NARCIS (Netherlands)

    Tan, S.D.; Xie, R.; Klein-Nulend, J.; Rheden, R.E.M. van; Bronckers, A.L.; Kuijpers-Jagtman, A.M.; Hoff, J.W. Von den; Maltha, J.C.

    2009-01-01

    Mechanosensitive osteocytes are essential for bone remodeling. Nitric oxide, an important regulator of bone remodeling, is produced by osteocytes through the activity of constitutive endothelial nitric oxide synthase (eNOS) or inducible nitric oxide synthase (iNOS). We hypothesized that these

  3. Association between nucleotide mutation of eNOS gene and serum ...

    African Journals Online (AJOL)

    Galaxy

    2013-05-15

    May 15, 2013 ... spasm among Japanese (Nakayama et al., 1999; Casas et al., 2006). It is believed that these mutations might result in altered NO metabolism and impaired .... ship between T-786C mutation of eNOS gene and CAD specifically in the Iranian population. To our knowledge, this polymorphism has never been ...

  4. Association of eNOS and HSP70 gene polymorphisms with glaucoma in Pakistani cohorts.

    NARCIS (Netherlands)

    Ayub, H.; Khan, M.I.; Micheal, S.; Akhtar, F.; Ajmal, M.; Shafique, S.; Ali, S.H.; Hollander, A.I. den; Ahmed, A.; Qamar, R.

    2010-01-01

    PURPOSE: To investigate the involvement of stress-regulating genes, endothelial nitric oxide synthase (eNOS) and heat shock protein 70 (HSP70) with primary open angle glaucoma (POAG) and primary closed angle glaucoma (PCAG). METHODS: POAG and PCAG patients recruited from different areas of Pakistan

  5. A meta-analysis of eNOS and ACE gene polymorphisms and risk of pre-eclampsia in women.

    Science.gov (United States)

    Shaik, A P; Sultana, A; Bammidi, V K; Sampathirao, K; Jamil, K

    2011-10-01

    A meta-analyses of endothelial nitric oxide synthase (eNOS) and angiotensin-converting enzyme (ACE) gene polymorphisms in pre-eclampsia was performed. We shortlisted 33 studies (17 for ACE; 16 for eNOS gene polymorphisms), of which 29 articles (16 for ACE and 15 for eNOS) were analysed. Overall, 1,620 cases with pre-eclampsia and 2,158 controls were analysed for intron 16 insertion-deletion polymorphism in ACE gene. A total of 1,610 subjects with pre-eclampsia and 2,875 controls were analysed for the Glu298Asp in eNOS gene. Overall, the random-effects odds ratio (OR) with Glu298Asp in eNOS gene was 0.958 (95% confidence intervals, CI 0.747-1.228, p > 0.05), and for the insertion-deletion/ACE polymorphism was 0.987 (95% CI 0.698-1.395, p > 0.05). Significant heterogeneity was observed in the studies that evaluated polymorphisms in ACE (Q value = 55.6; I(2) = 73; p value = 0.000); and eNOS (Q value = 37.2; I(2) = 62.4; p value = 0.001) polymorphisms. No significant risk of pre-eclampsia was observed in both eNOS and ACE genes with these polymorphisms.

  6. Gender, exercise training, and eNOS expression in porcine skeletal muscle arteries.

    Science.gov (United States)

    Laughlin, M Harold; Welshons, Wade V; Sturek, Michael; Rush, James W E; Turk, James R; Taylor, Julia A; Judy, Barbara M; Henderson, Kyle K; Ganjam, V K

    2003-07-01

    Our purpose was to determine the effects of gender and exercise training on endothelial nitric oxide synthase (eNOS) and superoxide dismutase (SOD) protein content of porcine skeletal muscle arteries and to evaluate the role of 17beta-estradiol (E2) in these effects. We measured eNOS and SOD content with immunoblots and immunohistochemistry in femoral and brachial arteries of trained and sedentary male and female pigs and measured estrogen receptor (ER) mRNA and alpha-ER and beta-ER protein in aortas of male and female pigs. Results indicate that female arteries contain more eNOS than male arteries and that exercise training increases eNOS content independent of gender. Male and female pigs expressed similar levels of alpha-ER mRNA and protein and similar amounts beta-ER protein in their arteries. E2 concentrations as measured by RIA were 180 +/- 34 pg/ml in male sera and approximately 5 pg/ml in female sera, and neither was changed by training. However, bioassay indicated that biologically active estrogen equivalent to only 35 +/- 5 pg/ml was present in male sera. E2 in female pigs, whether measured by RIA or bioassay, was approximately 24 pg/ml at peak estrous and 2 pg/ml on day 5 diestrus. The free fraction of E2 in sera did not explain the low measurements, relative to RIA, of E2. We conclude that 1). gender has significant influence on eNOS and SOD content of porcine skeletal muscle arteries; 2). the effects of gender and exercise training vary among arteries of different anatomic origin; 3). male sera contains compounds that cause RIA to overestimate circulating estrogenic activity; and 4). relative to human men, the male pig is not biologically estrogenized by high levels of E2 reported by RIA, whereas in female pigs E2 levels are lower than in the blood of human women.

  7. Teine Tallink või teine Olympic? / Villu Zirnask

    Index Scriptorium Estoniae

    Zirnask, Villu, 1966-

    2007-01-01

    Autor leiab, et risk, mis rikkus uusaktsionäride jaoks Tallinki IPO - vanad omanikud panid suure hulga oma aktsiaid peagi pärast firma börsile jõudmist müüki, on Ekspress Grupi aktsia puhul välistatud

  8. Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

    Energy Technology Data Exchange (ETDEWEB)

    Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri; Yamamori, Tohru [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan); Niwa, Koichi [Laboratory of Biochemistry, Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493 (Japan); Hattori, Yuichi [Department of Molecular and Medical Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Kondo, Takashi [Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Inanami, Osamu, E-mail: inanami@vetmed.hokudai.ac.jp [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan)

    2015-01-02

    Highlights: • eNOS activity is increased in BAECs exposed to X-rays. • ATM is involved in this increased eNOS activity. • HSP90 modulates the radiation-induced activation of ATM and eNOS. - Abstract: In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.

  9. Towards unification of the Vorticity Confinement and Shock Capturing (TVD and ENO/WENO) methods

    Science.gov (United States)

    Sidilkover, David

    2018-04-01

    New multidimensional extensions of the TVD and finite difference ENO/WENO methods for the compressible flow equations are proposed. The novelty of the approach is in the discretization schemes that acquire by means of a single mechanism both shock-capturing and vorticity confinement capabilities. Thus, the new method can be interpreted as a unification of the two methodologies, intended initially for different purposes.

  10. Morus alba extract modulates blood pressure homeostasis through eNOS signaling.

    Science.gov (United States)

    Carrizzo, Albino; Ambrosio, Mariateresa; Damato, Antonio; Madonna, Michele; Storto, Marianna; Capocci, Luca; Campiglia, Pietro; Sommella, Eduardo; Trimarco, Valentina; Rozza, Francesco; Izzo, Raffaele; Puca, Annibale A; Vecchione, Carmine

    2016-10-01

    Morus alba is a promising phytomedicine cultivated in oriental countries that is extensively used to prevent and treat various cardiovascular problems. To date, despite its beneficial effects, the molecular mechanisms involved remain unclear. Thus, we investigate the vascular and haemodynamic effects of Morus alba extract in an experimental model focusing our attention on the molecular mechanisms involved. Through vascular reactivity studies, we demonstrate that Morus alba extract evokes endothelial vasorelaxation through a nitric oxide-dependent pathway. Our molecular analysis highlights an increase in endothelial nitric oxide synthase (eNOS) phosphorylation. In vivo administration of Morus alba extract reduces blood pressure levels exclusively in wild-type mice, whereas it fails to evoke any haemodynamic effects in eNOS-deficient mice. Molecular analyses revealed that its beneficial action on vasculature is mediated by the activation of two important proteins that act as stress sensors and chaperones: PERK and heat shock protein 90. Finally, Morus alba extract exerts antihypertensive action in an experimental model of arterial hypertension. Through its action on eNOS signaling, Morus alba extract could act as a food supplement for the regulation of cardiovascular system, mainly in clinical conditions characterized by eNOS dysfunction, such as arterial hypertension. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    Directory of Open Access Journals (Sweden)

    Loo Keat

    2012-05-01

    Full Text Available Abstract Background Hyperhomocysteinemia as a consequence of the MTHFR 677 C > T variant is associated with cardiovascular disease and stroke. Another factor that can potentially contribute to these disorders is a depleted nitric oxide level, which can be due to the presence of eNOS +894 G > T and eNOS −786 T > C variants that make an individual more susceptible to endothelial dysfunction. A number of genotyping methods have been developed to investigate these variants. However, simultaneous detection methods using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP analysis are still lacking. In this study, a novel multiplex PCR-RFLP method for the simultaneous detection of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C variants was developed. A total of 114 healthy Malay subjects were recruited. The MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C variants were genotyped using the novel multiplex PCR-RFLP and confirmed by DNA sequencing as well as snpBLAST. Allele frequencies of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C were calculated using the Hardy Weinberg equation. Methods The 114 healthy volunteers were recruited for this study, and their DNA was extracted. Primer pair was designed using Primer 3 Software version 0.4.0 and validated against the BLAST database. The primer specificity, functionality and annealing temperature were tested using uniplex PCR methods that were later combined into a single multiplex PCR. Restriction Fragment Length Polymorphism (RFLP was performed in three separate tubes followed by agarose gel electrophoresis. PCR product residual was purified and sent for DNA sequencing. Results The allele frequencies for MTHFR 677 C > T were 0.89 (C allele and 0.11 (T allele; for eNOS +894 G > T, the allele frequencies were 0.58 (G allele and 0.43 (T allele; and for eNOS −786 T > C, the allele

  12. ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Xinghua; Miao, Xiaobing; Wu, Yaxun; Li, Chunsun; Guo, Yan; Liu, Yushan; Chen, Yali; Lu, Xiaoyun [Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu (China); Wang, Yuchan, E-mail: wangyuchannt@126.com [Department of Pathogen and Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu (China); He, Song, E-mail: hesongnt@126.com [Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu (China)

    2015-07-15

    Enolases are glycolytic enzymes responsible for the ATP-generated conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to the glycolytic function, Enolase 1 (ENO1) has been reported up-regulation in several tumor tissues. In this study, we investigated the expression and biologic function of ENO1 in Non-Hodgkin's Lymphomas (NHLs). Clinically, by western blot analysis we observed that ENO1 expression was apparently higher in diffuse large B-cell lymphoma than in the reactive lymphoid tissues. Subsequently, immunohistochemical staining of 144 NHLs suggested that the expression of ENO1 was significantly lower in the indolent lymphomas compared with the progressive lymphomas. Further, we identified ENO1 as an independent prognostic factor, and it was significantly correlated with overall survival of NHL patients. In addition, we found that ENO1 could promote cell proliferation, regulate cell cycle associated gene and PI3K/AKT signaling pathway in NHLs. Finally, we verified that ENO1 participated in the process of lymphoma cell adhesion mediated drug resistance (CAM-DR). Adhesion to FN or HS5 cells significantly protected OCI-Ly8 and Daudi cells from cytotoxicity compared with those cultured in suspension, and these effects were attenuated when transfected with ENO1-siRNA. Based on the study, we propose that inhibition of ENO1 expression may be a novel strategy for therapy for NHLs patients, and it may be a target for drug resistance. - Highlights: • ENO1 expression is reversely correlated with clinical outcomes of patients with NHLs. • ENO1 promotes the proliferation of NHL cells. • ENO1 regulates cell adhesion mediated drug resistance.

  13. KONSUMSI MIKRONUTRIEN DAN EKSPRESI GEN eNOS3 ALEL INTRO 4a4b PADA PENDERITA HIPERTENSI ETNIK MINANGKABAU

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    Delmi Sulastri

    2011-05-01

    Full Text Available AbstrakGen eNOS3 varian intron 4a4b merupakan salah satu gen yang berhubungan dengan kejadian hipertensi esensial. Kemampuan Gen eNOS3 varian ini untuk mensintesis enzim nitric oxide synthase (NOS berkurang sehingga sintesis Nitric oxide (NO menurun. Senyawa NO adalah suatu senyawa vasoaktif yang menyebabkan vasodilatasi pembuluh darah.Penelitian pada subjek hipertensi dan normotensi berusia 30-65 tahun bertujuan untuk melihat pengaruh mikronutrien terhadap ekspresi gen eNOS3 alel intron 4a4b. 130 subjek, diwawancara pola makannya lalu dilakukan analisis asupan mikronutrien, gen eNOS3, serta kadar NO plasma. Data yang diperoleh dianalisis menggunakan uji T dan chi-square.Kadar NO plasma penderita hipertensi adalah 26.91 + 15.40 μM/L dan normotensi 25.79 + 15.04 μM/L. 55.5% subjek hipertensi memiliki gen eNOS3 varian intron 4b dan 69% subjek normotensi mempunyai varian intron 4a4b. Kadar NO plasma rendah, (66.7% ditemukan pada subjek hipertensi dengan varian intron 4b. Tidak terdapat hubungan bermakna asupan mikronutrien dengan kadar NO plasma pada subjek hipertensi.Hasil ini tidak mendukung pengaruh konsumsi mikronutrien terhadap kadar NO plasma pada penderita hipertensi dengan gen eNOS3 varian intron 4a4b.Kata Kunci : hipertensi esensial, gen eNOS3, kadar NO plasmaAbstracteNOS 3 gene intron 4a4b allel is one of the important genes which is related to hypertesion essensial. This gene expresses the nitric oxide synthase (NOS enzyme which regulates the synthesis of Nitric oxide (NO in human body. NO causes vasodilatation, which decreases peripheral resistant and blood pressure.A research has been done based on cross sectional study on hypertension patients and those with normal blood pressure in the range of 30-65 years old. The main of this research is to evaluate the influence of micronutrient consumption on eNOS3 gene intron 4a4b allel expression in hypertension subjects in Minangkabau ethnicity. 130 subjects interviewed about micronutrient

  14. Disruption of COX-2 and eNOS does not confer protection from cardiovascular failure in lipopolysaccharide-treated conscious mice and isolated vascular rings

    DEFF Research Database (Denmark)

    Stæhr, Mette; Madsen, Kirsten; Vanhoutte, Paul M

    2011-01-01

    (NS 398), disruption of COX-2, endothelium removal, or eNOS deletion (eNOS(-/-)) did not improve vascular reactivity after LPS, while the NO synthase blockers 1400W and N(G)-nitro-l-arginine methyl ester prevented loss of tone. COX-2 and eNOS activities are not necessary for LPS-induced decreases...... in blood pressure, heart rate, and vascular reactivity. Inducible NOS activity appears crucial. COX-2 and eNOS are not obvious therapeutic targets for cardiovascular rescue during gram-negative endotoxemic shock....

  15. Alpha-enolase (ENO1 controls alpha v/beta 3 integrin expression and regulates pancreatic cancer adhesion, invasion, and metastasis

    Directory of Open Access Journals (Sweden)

    Moitza Principe

    2017-01-01

    Full Text Available Abstract Background We have previously shown that in pancreatic ductal adenocarcinoma (PDA cells, the glycolytic enzyme alpha-enolase (ENO1 also acts as a plasminogen receptor and promotes invasion and metastasis formation. Moreover, ENO1 silencing in PDA cells induces oxidative stress, senescence and profoundly modifies PDA cell metabolism. Although anti-ENO1 antibody inhibits PDA cell migration and invasion, little is known about the role of ENO1 in regulating cell-cell and cell-matrix contacts. We therefore investigated the effect of ENO1 silencing on the modulation of cell morphology, adhesion to matrix substrates, cell invasiveness, and metastatic ability. Methods The membrane and cytoskeleton modifications that occurred in ENO1-silenced (shENO1 PDA cells were investigated by a combination of confocal microscopy and atomic force microscopy (AFM. The effect of ENO1 silencing was then evaluated by phenotypic and functional experiments to identify the role of ENO1 in adhesion, migration, and invasion, as well as in senescence and apoptosis. The experimental results were then validated in a mouse model. Results We observed a significant increase in the roughness of the cell membrane due to ENO1 silencing, a feature associated with an impaired ability to migrate and invade, along with a significant downregulation of proteins involved in cell-cell and cell-matrix adhesion, including alpha v/beta 3 integrin in shENO1 PDA cells. These changes impaired the ability of shENO1 cells to adhere to Collagen I and IV and Fibronectin and caused an increase in RGD-independent adhesion to vitronectin (VN via urokinase plasminogen activator receptor (uPAR. Binding of uPAR to VN triggers integrin-mediated signals, which result in ERK1-2 and RAC activation, accumulation of ROS, and senescence. In shENO1 cancer cells, the use of an anti-uPAR antibody caused significant reduction of ROS production and senescence. Overall, a decrease of in vitro and in vivo cell

  16. Enhancing eNOS activity with simultaneous inhibition of IKKβ restores vascular function in Ins2(Akita+/-) type-1 diabetic mice.

    Science.gov (United States)

    Krishnan, Manickam; Janardhanan, Preethi; Roman, Linda; Reddick, Robert L; Natarajan, Mohan; van Haperen, Rien; Habib, Samy L; de Crom, Rini; Mohan, Sumathy

    2015-10-01

    The balance of nitric oxide (NO) versus superoxide generation has a major role in the initiation and progression of endothelial dysfunction. Under conditions of high glucose, endothelial nitric oxide synthase (eNOS) functions as a chief source of superoxide rather than NO. In order to improve NO bioavailability within the vessel wall in type-1 diabetes, we investigated treatment strategies that improve eNOS phosphorylation and NO-dependent vasorelaxation. We evaluated methods to increase the eNOS activity by (1) feeding Ins2(Akita) spontaneously diabetic (type-1) mice with l-arginine in the presence of sepiapterin, a precursor of tetrahydrobiopterin; (2) preventing eNOS/NO deregulation by the inclusion of inhibitor kappa B kinase beta (IKKβ) inhibitor, salsalate, in the diet regimen in combination with l-arginine and sepiapterin; and (3) independently increasing eNOS expression to improve eNOS activity and associated NO production through generating Ins2(Akita) diabetic mice that overexpress human eNOS predominantly in vascular endothelial cells. Our results clearly demonstrated that diet supplementation with l-arginine, sepiapterin along with salsalate improved phosphorylation of eNOS and enhanced vasorelaxation of thoracic/abdominal aorta in type-1 diabetic mice. More interestingly, despite the overexpression of eNOS, the in-house generated transgenic eNOS-GFP (TgeNOS-GFP)-Ins2(Akita) cross mice showed an unanticipated effect of reduced eNOS phosphorylation and enhanced superoxide production. Our results demonstrate that enhancement of endogenous eNOS activity by nutritional modulation is more beneficial than increasing the endogenous expression of eNOS by gene therapy modalities.

  17. Endothelial CaMKII as a regulator of eNOS activity and NO-mediated vasoreactivity.

    Directory of Open Access Journals (Sweden)

    Shubha Murthy

    Full Text Available The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII is a serine/threonine kinase important in transducing intracellular Ca2+ signals. While in vitro data regarding the role of CaMKII in the regulation of endothelial nitric oxide synthase (eNOS are contradictory, its role in endothelial function in vivo remains unknown. Using two novel transgenic models to express CaMKII inhibitor peptides selectively in endothelium, we examined the effect of CaMKII on eNOS activation, NO production, vasomotor tone and blood pressure. Under baseline conditions, CaMKII activation was low in the aortic wall. Consistently, systolic and diastolic blood pressure, heart rate and plasma NO levels were unaltered by endothelial CaMKII inhibition. Moreover, endothelial CaMKII inhibition had no significant effect on NO-dependent vasodilation. These results were confirmed in studies of aortic rings transduced with adenovirus expressing a CaMKII inhibitor peptide. In cultured endothelial cells, bradykinin treatment produced the anticipated rapid influx of Ca2+ and transient CaMKII and eNOS activation, whereas CaMKII inhibition blocked eNOS phosphorylation on Ser-1179 and dephosphorylation at Thr-497. Ca2+/CaM binding to eNOS and resultant NO production in vitro were decreased under CaMKII inhibition. Our results demonstrate that CaMKII plays an important role in transient bradykinin-driven eNOS activation in vitro, but does not regulate NO production, vasorelaxation or blood pressure in vivo under baseline conditions.

  18. Sildenafil promotes eNOS activation and inhibits NADPH oxidase in the transgenic sickle cell mouse penis.

    Science.gov (United States)

    Musicki, Biljana; Bivalacqua, Trinity J; Champion, Hunter C; Burnett, Arthur L

    2014-02-01

    Sickle cell disease (SCD)-associated vasculopathy in the penis is characterized by aberrant nitric oxide and phosphodiesterase (PDE) 5 signaling, and by increased oxidative stress. Preliminary clinical trials show that continuous treatment with PDE5 inhibitor sildenafil unassociated with sexual activity decreases priapic activity in patients with SCD. However, the mechanism of its vasculoprotective effect in the penis remains unclear. We evaluated whether continuous administration of PDE5 inhibitor sildenafil promotes eNOS function at posttranslational levels and decreases superoxide-producing enzyme NADPH oxidase activity in the sickle cell mouse penis. SCD transgenic mice were used as an animal model of SCD. WT mice served as controls. Mice received treatment with the PDE5 inhibitor sildenafil (100 mg/kg/day) or vehicle for 3 weeks. eNOS phosphorylation on Ser-1177 (positive regulatory site), eNOS interactions with heat-shock protein 90 (HSP90) (positive regulator), phosphorylated AKT (upstream mediator of eNOS phosphorylation on Ser-1177), an NADPH oxidase catalytic subunit gp91(phox), and a marker of oxidative stress (4-hydroxy-2-nonenal [HNE]) were measured by Western blot. Effect of continuous sildenafil treatment on eNOS posttranslational activation, NADPH oxidase catalytic subunit, and oxidative stress in the penis of the sickle cell mouse. Continuous treatment with sildenafil reversed (P penis. Sildenafil treatment of WT mice did not affect any of these parameters. Our findings that sildenafil enhances eNOS activation and inhibits NADPH oxidase function in the sickle cell mouse penis offers a vasculoprotective molecular basis for the therapeutic effect of sildenafil in the penis in association with SCD. © 2013 International Society for Sexual Medicine.

  19. Partial deletion of eNOS gene causes hyperinsulinemic state, unbalance of cardiac insulin signaling pathways and coronary dysfunction independently of high fat diet.

    Directory of Open Access Journals (Sweden)

    Cecilia Vecoli

    Full Text Available Abnormalities in eNOS gene, possibly interacting with high fat diet (HFD, affect peripheral vascular function and glucose metabolism. The relative role of eNOS gene, HFD and metabolic derangement on coronary function has not been fully elucidated. We test whether eNOS gene deficiency per se or in association with HFD modulates coronary function through mechanisms involving molecular pathways related to insulin signaling. Wild type (WT, eNOS-/- and eNOS+/- mice were studied. WT and eNOS+/- mice were fed with either standard or HF diet for 16 weeks and compared with standard diet fed eNOS-/-. Glucose and insulin tolerance tests were performed during the last week of diet. Coronary resistance (CR was measured at baseline and during infusions of acetylcholine (Ach or sodium-nitroprusside (SNP to evaluate endothelium-dependent or independent vasodilation, in the Langendorff isolated hearts. Cardiac expression of Akt and ERK genes as evaluation of two major insulin-regulated signaling pathways involved in the control of vascular tone were assessed by western blot. HFD-fed mice developed an overt diabetic state. Conversely, chow-fed genetically modified mice (in particular eNOS-/- showed a metabolic pattern characterized by normoglycemia and hyperinsulinemia with a limited degree of insulin resistance. CR was significantly higher in animals with eNOS gene deletions than in WT, independently of diet. Percent decrease in CR, during Ach infusion, was significantly lower in both eNOS-/- and eNOS+/- mice than in WT, independently of diet. SNP reduced CR in all groups except eNOS-/-. The cardiac ERK1-2/Akt ratio, increased in animals with eNOS gene deletions compared with WT, independently of diet. These results suggest that the eNOS genetic deficiency, associated or not with HFD, has a relevant effect on coronary vascular function, possibly mediated by increase in blood insulin levels and unbalance in insulin-dependent signaling in coronary vessels

  20. Environmental Aspects as Assessment Criteria in Municipal Heat Energy Decisions - Case of Eno Energy Cooperative

    Energy Technology Data Exchange (ETDEWEB)

    Puhakka, Asko [North Karelia Univ. of Applied Sciences, Joensuu (Finland)

    2006-07-15

    The aim of this paper is to provide information whether it is possible to consider the sustainable development perspectives in the decision making of the district energy decision. The new EU-directives concerning public procurements allow the use of environmental aspects as selection criteria. The focus here is on small-scale district heating systems and their fuel-supply chains. The comparable fuels included the analysis are forest chips, heavy fuel oil, light fuel oil and peat. The paper focuses to the concept of the sustainable development and establishes the indicators for ecological-, social- and economical aspects of the district heating. The indicators are utilized in the case study on the Eno Energy Cooperative. The equivalent CO{sub 2} emissions from the production and the combustion of the fuel, the employment impacts of the fuel production and the formation of the price of energy for the consumers are considered. After presenting the sustainable development indicators in the case of Eno Energy Cooperative, the investment models of heat entrepreneurship business are discussed. Finally, we also raise an attention into important aspects to be considered when establishing a local district heating scheme. The indicators used in this presentation show that the use of forest chips in energy production has positive effect through the reduced greenhouse gases. The use of wood in energy production also provides employment opportunities and is more favourable to consumers, because of the steady fuel price when compared to other alternative fuels.

  1. Vasoinhibins Prevent Bradykinin-Stimulated Endothelial Cell Proliferation by Inactivating eNOS via Reduction of both Intracellular Ca2+ Levels and eNOS Phosphorylation at Ser1179

    Directory of Open Access Journals (Sweden)

    Carmen Clapp

    2011-07-01

    Full Text Available Vasoinhibins, a family of antiangiogenic peptides derived from prolactin proteolysis, inhibit the vascular effects of several proangiogenic factors, including bradykinin (BK. Here, we report that vasoinhibins block the BK-induced proliferation of bovine umbilical vein endothelial cells. This effect is mediated by the inactivation of endothelial nitric oxide synthase (eNOS, as the NO donor DETA-NONOate reverted vasoinhibin action. It is an experimentally proven fact that the elevation of intracellular Ca2+ levels ([Ca2+]i upon BK stimulation activates eNOS, and vasoinhibins blocked the BK-mediated activation of phospholipase C and the formation of inositol 1,4,5-triphosphate leading to a reduced release of Ca2+ from intracellular stores. The [Ca2+]i rise evoked by BK also involves the influx of extracellular Ca2+ via canonical transient receptor potential (TRPC channels. Vasoinhibins likely interfere with TRPC-mediated Ca2+ entry since La3+, which is an enhancer of TRPC4 and TRPC5 channel activity, prevented vasoinhibins from blocking the stimulation by BK of endothelial cell NO production and proliferation, and vasoinhibins reduced the BK-induced increase of TRPC5 mRNA expression. Finally, vasoinhibins prevented the BK-induced phosphorylation of eNOS at Ser1179, a post-translational modification that facilitates Ca2+-calmodulin activation of eNOS. Together, our data show that vasoinhibins, by lowering NO production through the inhibition of both [Ca2+]i mobilization and eNOS phosphorylation, prevent the BK-induced stimulation of endothelial cell proliferation. Thus, vasoinhibins help to regulate BK effects on angiogenesis and vascular homeostasis.

  2. Effects of hyperbaric oxygen therapy in enhancing expressions of e-NOS, TNF-α and VEGF in wound healing

    Science.gov (United States)

    Susilo, Imam; Devi, Anita; Purwandhono, Azham; Hadi Warsito, Sunaryo

    2017-05-01

    Wound healing is a physiological process that occurs progressively through overlapping phases. Tissue oxygenation is an important part of the complex regulation for wound healing. Hyperbaric Oxygen (HBO) therapy is a method of increasing oxygen delivery to tissues. The therapy improves tissue oxygenation and stimulates the formation of H2O2 as a secondary messenger for Tumour Necrosis Factor alpha (TNF α), e-NOS, VEGF and Nuclear Factor Kappa Beta phosphorylation (NF-Kb) which play an important role in the rapid transcription of a wide variety of genes in response to extracellular stimuli. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of e-NOS, TNF-α, VEGF and wound healing. This study is an animal study with a ‘randomized control group of pre-test and post test design’ on 28 Wistar rats. Randomly, the rats were divided into 4 groups with 7 rats in each group. The HBO treatment group 1 received 5 sessions of HBO 2.4 ATA in 3 × 30 minutes; the HBO treatment group 2 received 10 sessions of HBO 2.4 ATA in 3 × 30 minutes; and each of the control groups were without HBO. Each of the 28 male rats were given a full thickness excisional wound of 1 × 1cm. Examinations of e-NOS, TNF-α, VEGF expressions and wound healing were performed on day-0 (pre-HBO) and day-5 HBO or on day-0 (pre-HBO) and day-10 HBO. The resultsshowthat the Hyperbaric Oxygen therapy can improve e-NOS (p=0.02), TNF-α (p= 0.02), VEGF expression (p=0.02) and wound healing (p=0.002) significantly in the provision of HBO 2.4 ATA for 3 × 30 minutes in 5 sessions over 5 consecutive days. While the 10 sessions of HBO 2.4 ATA for 3 × 30 minutes over 10 consecutive days only increase e-NOS (p=0.02), TNF-α (p=0.04), VEGF expression significantly (p=0.03) but do not improve wound healing significantly (p=0.3) compared with no HBO. The study concludes that HBO can improve the expressions of e-NOS, TNF-α, VEGF and wound healing in the provision of HBO

  3. An Eulerian method for computation of multimaterial impact with ENO shock-capturing and sharp interfaces

    CERN Document Server

    Udaykumar, H S; Belk, D M; Vanden, K J

    2003-01-01

    A technique is presented for the numerical simulation of high-speed multimaterial impact. Of particular interest is the interaction of solid impactors with targets. The computations are performed on a fixed Cartesian mesh by casting the equations governing material deformation in Eulerian conservation law form. The advantage of the Eulerian setting is the disconnection of the mesh from the boundary deformation allowing for large distortions of the interfaces. Eigenvalue analysis reveals that the system of equations is hyperbolic for the range of materials and impact velocities of interest. High-order accurate ENO shock-capturing schemes are used along with interface tracking techniques to evolve sharp immersed boundaries. The numerical technique is designed to tackle the following physical phenomena encountered during impact: (1) high velocities of impact leading to large deformations of the impactor as well as targets; (2) nonlinear wave-propagation and the development of shocks in the materials; (3) modelin...

  4. Identification and Function Analysis of enolase Gene NlEno1 from Nilaparvata lugens (Stål) (Hemiptera:Delphacidae)

    Science.gov (United States)

    Wang, Wei-Xia; Li, Kai-Long; Chen, Yang; Lai, Feng-Xiang; Fu, Qiang

    2015-01-01

    The enolase [EC 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-PGE) to phosphoenolpyruvate (PEP). In this study, a full-length cDNA encoding α-enolase was cloned from rice brown planthopper (Nilaparvata lugens) and is provisionally designated as NlEno1. The cDNA sequence of NlEno1 was 1,851 bp with an open reading frame (ORF) of 1,305 bp and encoding 434 amino acids. The deduced protein shares high identity of 80–87% with ENO1-like protein from Hemiptera, Diptera, and Lepidoptera speices. The NlEno1 showed the highest mRNA expression level in hemolymph, followed by fat body, salivary gland, ovaries and egg, and showed trace mRNA levels in testis. The mRNA of NlEno1 showed up-regulated level in virulent N. lugens population Mudgo, IR56 and IR42 when compared with TN1 population. Injection of double-stranded RNA (dsRNA) of NlEno1 into the adults significantly down-regulated the NlEno1 mRNA level along with decreased eggs and offspring. Moreover, injection of NlEno1-dsRNA decreased mRNA level of Vitellogenin (Vg) gene. These results showed that the NlEno1, as a key glycolytic enzyme, may play roles in regulation of fecundity and adaptation of N. lugens to resistant rice varieties. PMID:26056319

  5. Endothelial dysfunction in children with obstructive sleep apnea is associated with epigenetic changes in the eNOS gene.

    Science.gov (United States)

    Kheirandish-Gozal, Leila; Khalyfa, Abdelnaby; Gozal, David; Bhattacharjee, Rakesh; Wang, Yang

    2013-04-01

    Obstructive sleep apnea (OSA) is a highly prevalent disorder that has been associated with an increased risk for cardiovascular morbidity, even in children. However, not all children with OSA manifest alterations in endothelial postocclusive hyperemia, an endothelial nitric oxide synthase (eNOS)-dependent response. Since expression of the eNOS gene is regulated by epigenetic mechanisms and OSA may cause epigenetic modifications such as DNA hypermethylation, we hypothesized that epigenetic modifications in the eNOS gene may underlie the differential vascular phenotypes in pediatric OSA. Age-, sex-, ethnicity-, and BMI-matched prepubertal children with polysomnographically confirmed OSA and either normal (OSAn) or abnormal (OSAab) postocclusive hyperemic responses, assessed as the time to attain peak reperfusion flow (Tmax) by laser Doppler flowmetry, were recruited. Blood genomic DNA was assessed for epigenetic modifications in the eNOS gene using pyrosequencing. Children with no evidence of OSA or endothelial dysfunction served as a control group. The study comprised 36 children with OSA (11 with OSAab and 25 with OSAn) and 35 children in the control group. Overall, the mean age was 7.5 ± 2.4 years, 65% were boys, and 30% were obese; mean apnea-hypopnea index was 18 ± 8.6/h of sleep for the children with OSA. Tmax was 66.7 ± 8.8 s in the OSAab group and 30.1 ± 8.3 s in the OSAn group (P < .001). Pyrosequencing of the proximal promoter region of the eNOS gene revealed no significant differences in six of the seven CpG sites. However, a CpG site located at position -171 (relative to transcription start site), approximating important transcriptional elements, displayed significantly higher methylation levels in the OSAab group as compared with the OSAn or control groups (81.5% ± 3.5%, 74.8% ± 1.4%, and 74.5% ± 1.7%, respectively; P < .001). eNOS mRNA expression levels were assessed in a separate group of children and were significantly reduced in the OSAab

  6. Estrogen Receptor Signaling and the PI3K/Akt Pathway Are Involved in Betulinic Acid-Induced eNOS Activation

    Directory of Open Access Journals (Sweden)

    Nicolas Hohmann

    2016-07-01

    Full Text Available Betulinic acid (BA is a naturally occurring pentacyclic triterpenoid with anti-inflammatory, antiviral and anti-cancer properties. Beneficial cardiovascular effects such as increased nitric oxide (NO production through enhancement of endothelial NO synthase (eNOS activity and upregulation of eNOS expression have been demonstrated for this compound. In the present study, immortalized human EA.hy 926 endothelial cells were incubated for up to 1 h with 1–100 µM BA and with the phosphatidylinositol-3-kinase (PI3K inhibitors LY294002 and wortmannin, or the estrogen receptor (ER antagonist ICI 182,780. Phosphorylation status of eNOS and total eNOS protein were analyzed by Western blotting using a serine 1177 phosphosite-specific antibody. Bioactive NO production was assessed by determination of cGMP content in rat lung fibroblasts (RFL-6 reporter cells. Short-term incubation of EA.hy 926 cells with BA resulted in eNOS phosphorylation at the serine 1177 residue in a concentration- and time-dependent manner with a half-maximal effective concentration of 0.57 µM. This was associated with an enhanced production of NO. BA-induced eNOS phosphorylation and NO production was completely blocked by pretreatment with ICI 182,780, and was attenuated by pretreatment with the PI3K inhibitors wortmannin and LY294002. These results indicate that fast non-genomic effects of ER with downstream signaling through the PI3K/Akt pathway and consecutive eNOS phosphorylation at serine 1177 are involved in BA-induced eNOS activation.

  7. Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency

    OpenAIRE

    Fallahi, Ali Asghar; Shekarfroush, Shahnaz; Rahimi, Mostafa; Jalali, Amirhossain; Khoshbaten, Ali

    2016-01-01

    Objective(s): High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Mat...

  8. FEATURES OF THE CLINICAL SIGNIFICANCE OF POLYMORPHIC VARIANTS OF ENOS AND AGTR2 GENES IN PATIENTS WITH CAD

    Directory of Open Access Journals (Sweden)

    A. L. Khokhlov

    2016-01-01

    Full Text Available Coronary heart disease (CHD is a major cause of mortality. Morphological substrate of CHD in most cases is atherosclerosis, which is based on structural genes polymorphism eNOS and AGTR2. The aim of the study was to study the prevalence of eNOS and AGTR2 genes in patients with coronary artery disease and the association of these genes with coronary heart disease. The study involved 187 patients aged 36 to 86 years (62,2±11,2 with different forms of CHD: stable and unstable angina, myocardial infarction and 45 people without CHD. Determination of gene polymorphisms was performed by real-time PCR analyzer of nucleic acids IQ 5 Bio-Rad. Statistical analysis was performed using Statistica 10.0. The study revealed a significant difference between the incidence of homozygous AA allelic variant gene AGTR2 group of patients with myocardial infarction and the comparison group; polymorphic variant AA AGTR2 gene is associated with earlier onset of coronary artery disease; It found that carriers of the polymorphic variant gene GA AGTR2 beginning statistically CHD occurred significantly later than in carriers of alleles GG and AA; age CHD debut TT allele carriers of the eNOS gene is associated with an earlier onset of the disease and statistically significantly different from the age of first CHD in carriers of alleles of polymorphic variants of GG and GT; revealed a positive correlation between the polymorphic allele AGTR2 gene with the presence of arterial hypertension in patients with coronary artery disease; It determined that the T allele carriers of the polymorphic gene eNOS is associated more early onset of hypertension, found the association of the polymorphic allele gene AGTR2 the need to use higher doses of ACE inhibitor — perindopril.

  9. Immunomodulatory effects of royal jelly on aorta CD3, CD68 and eNOS expression in hypercholesterolaemic rats

    Directory of Open Access Journals (Sweden)

    Amal Attia El-Morsy Ibrahim

    2014-08-01

    RJ administration (300 mg/kg/day with CH, produced a counteractive effect, represented in recompense of biochemical alteration, CD4 and CD8 expression. RJ intake also amended the histological picture. The immunohistochemical picture revealed a decrease in CD3, CD86 and eNOS in the aortic tissue. These findings attributed to the significant immunomodulatory effect of RJ remedy suppress deleterious effects of hypercholesterolaemia.

  10. Prevalence of endothelial nitric oxide synthase (eNOS) gene exon 7 Glu298Asp variant in North Eastern India

    Science.gov (United States)

    Shankarishan, Priyanka; Borah, Prasanta Kumar; Ahmed, Giasuddin; Mahanta, Jagadish

    2011-01-01

    Background & objectives Endothelial nitric oxide is a potent vasodilator and impairment of its generation brought about by gene polymorphism is considered a major predictor for several diseases. A single nucleotide polymorphism G894T within exon 7 of endothelial nitric oxide synthase (eNOS-7) gene, resulting in a replacement of glutamic acid by aspartic acid, has been studied as a putative candidate gene for cardiovascular diseases. The pattern of eNOS-7 Glu298Asp variant in the Indian population is poorly known. The present study was planned to determine the prevalence of the variant of this gene among tea garden community in Assam, North-East India with high prevalence of hypertension. Methods Study participants of both sex aged ≥18 yr were recruited randomly from temporary field clinics established in tea gardens of Dibrugarh, Assam. Genomic DNA was extracted from 409 subjects by the conventional phenol-chloroform method. The prevalence of the eNOS exon 7 Glu298Asp variant was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results The study population was in Hardy-Weinberg Equilibrium. The frequency of the eNOS GG, GT and TT genotypes was found to be 75, 22 and 3 per cent respectively and did not show any significant difference in gender wise analysis. Interpretation & conclusions Our results showed that the prevalence of the homozygous GG genotype was high (75%) and the rare mutant genotype (homozygous, TT) was 3 per cent in a population at risk with cardiovascular disease. Such population-based data on various polymorphisms can ultimately be exploited in pharmacogenomics. PMID:21623032

  11. Substance P ameliorates tumor necrosis factor-alpha-induced endothelial cell dysfunction by regulating eNOS expression in vitro.

    Science.gov (United States)

    Piao, Jiyuan; Hong, Hyun Sook; Son, Youngsook

    2018-04-01

    The aim of this study was to explore the beneficial effects of SP on NO production and inflammation-induced vascular endothelium cell death. To mimic the inflammatory environment, TNF-α was treated with HUVECs, and SP was added prior to TNF-α to determine its protective effect. WST-1 assay was performed to detect cell viability. NO level in conditioned medium was measured by Griess Reagent System. The protein level of cleaved caspase-3, eNOS, and phosphorylated Akt was detected by Western blot analysis. TNF-α declined endothelial cell viability by downregulating Akt and NO production. TNF-α-induced cell death was reliably restored by NO, confirming the requirement of NO for cell survival. By contrast, pretreatment of SP attenuated TNF-α-induced cellular apoptosis, accompanied by an increase in the phosphorylation of Akt, eNOS expression, and NO production. Blockage of NK-1R, phosphorylated Akt or eNOS by CP-96345, A6730, or L-NAME entirely eliminated the effect of SP. SP can protect the vascular endothelium against inflammation-induced damage through modulation of the Akt/eNOS/NO signaling pathway. © 2018 John Wiley & Sons Ltd.

  12. Efectos ecológicos del fenómeno ENOS en Colombia

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    Yesid Carvajal

    2014-06-01

    Full Text Available El fenómeno ENOS, tiene un sustancial efecto sobre las variaciones del clima a nivel mundial; por lo tanto, no sorprende que sus efectos tengan un impacto ecológico y socioeconómico fuerte, alterando la seguridad alimentaria de los países a nivel mundial. Ante este panorama, es comprensible que haya despertado el mayor interés mundial, no sólo en los especialistas en meteorología y oceanografía sino también entre biólogos, ecólogos, físicos, matemáticos, agrónomos, hidrólogos, economistas, sociólogos y planificadores, entre otros. En el presente documento, se muestra algunos de los principales efectos ecológicos en Colombia durante las fases extremas cálida (El Niño y fría (La Niña, indicando que es una necesidad urgente, orientar la investigación en sus causas, consecuencias e impactos.

  13. Association of eNOS and ACE gene polymorphisms and plasma nitric oxide with risk of non-small cell lung cancer in South India.

    Science.gov (United States)

    Peddireddy, Vidyullatha; Badabagni, Siva Prasad; Gundimeda, Sandhya Devi; Mundluru, Hema Prasad

    2018-01-01

    The role of ACE and eNOS gene polymorphisms and their association with various cancers were reported. However, their role in the lung cancer is unclear. In this study, we analyzed eNOS and ACE gene polymorphisms and the risk of non-small cell lung cancer (NSCLC) in South Indian population. For the eNOS gene, the homozygous "AA" genotypic frequency was significantly associated with NSCLC with an overall risk of 3.6-fold (P = 0.006, odds ratio = 3.58, 95% confidence interval = 1.66, 7.723). The heterozygous "I/D" genotypic frequency of ACE gene was significantly higher in NSCLC patients when compared to the controls with a 2.29-fold risk for NSCLC. Multiple regression analyses indicated that gender, smoking status, and polymorphisms in eNOS and ACE genes as the strongest predicting factors for an increased susceptibility to NSCLC. We report for the first time that polymorphisms in the eNOS "A/A" (homozygous mutant) and ACE "I/D" genotypes might contribute to the increased risk of NSCLC in the South Indian population. © 2016 John Wiley & Sons Ltd.

  14. Investigation of gene expression and serum levels of PIN1 and eNOS with high blood pressure in patients with Alzheimer disease.

    Science.gov (United States)

    Azimi, Mina; Nikanfar, Masoud; Khakikhatibi, Fatemeh; Rahbarghazi, Reza; Nourazarian, Seyed Manuchehr; Biray Avci, Cigir; Nourazarian, Alireza

    2017-09-01

    According to evidence, Alzheimer's disease is known as one of the most serious neurodegenerative diseases, for which hypertension has been observed to be a key risk factor. Therefore, this study aims to examine the relationship between the PIN1 and eNOS genes expression, as well as serum levels and hypertension in Alzheimer's disease sufferers. Blood samples were obtained from subjects who were divided into four groups: the control group, normotensive Alzheimer's patients, the Alzheimer's sufferers group with hypertension, and the healthy group with only hypertension, considering the inhibition of confounding factors. Thereafter, eNOS and PIN1 genes expression along with serum levels were studied. Based on the obtained results, a statistically significant correlation didn't exist between serum level of PIN1 and the systolic and diastolic blood pressure, between serum level of eNOS and diastolic blood pressure in the norm tension Alzheimer's disease patients, between serum levels of PIN1, eNOS and systolic blood pressure, and between serum eNOS and systolic and diastolic blood pressure in the patients with hypertension (pdiseases in Alzheimer's disease. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Effects of atorvastatin and T-786C polymorphism of eNOS gene on plasma metabolic lipid parameters.

    Science.gov (United States)

    Zago, Vanessa Helena de Souza; Santos, José Eduardo Tanus dos; Danelon, Mirian Regina Gardin; Silva, Roger Marcelo Mesquita da; Panzoldo, Natália Baratella; Parra, Eliane Soler; Alexandre, Fernanda; Virgínio, Vítor Wilson de Moura; Quintão, Eder Carlos Rocha; Faria, Eliana Cotta de

    2013-01-01

    Endothelial nitric oxide synthase (eNOS) activity may be modulated by high-density lipoprotein cholesterol (HDL-C), statins or polymorphisms, such as the T-786C of eNOS. This study aimed at evaluating if the T-786C polymorphism is associated with changes of atorvastatin effects on the lipid profile, on the concentrations of metabolites of nitric oxide (NO) and of high sensitivity C-reactive protein (hsCRP). Thirty male volunteers, asymptomatic, aged between 18 and 56 years were genotyped and classified according to absence (TT, n = 15) or presence (CC, n = 15) of the polymorphism. They were randomly selected for the use of placebo or atorvastatin (10 mg/day/14 days). After each treatment lipids, lipoproteins, HDL2 and HDL3 composition, cholesteryl ester transfer protein (CETP) activity, metabolites of NO and hsCRP were evaluated. The comparisons between genotypes after placebo showed an increase in CETP activity in a polymorphism-dependent way (TT, 12±7; CC, 22±12; p < 0.05). The interaction analyses between treatments indicated that atorvastatin has an effect on cholesterol, LDL, nitrite and lipid-protein ratios (HDL2 and HDL3) (p < 0.001) in both genotypes. Interestingly, we observed genotype/drug interactions on CETP (p < 0.07) and lipoprotein (a) (Lp(a)) (p < 0.056), leading to a borderline decrease in CETP, but with no effect on Lp(a). HsCRP showed no alteration. These results suggest that statin treatment may be relevant for primary prevention of atherosclerosis in patients with the T-786C polymorphism of eNOS, considering the effects on lipid metabolism.

  16. eNOS se correlaciona com a biogênese mitocondrial em corações com cardiopatia congênita e cianose eNOS correlates with mitochondrial biogenesis in hearts of congenital heart disease with cyanosis

    Directory of Open Access Journals (Sweden)

    Juan Xiao

    2012-09-01

    Full Text Available FUNDAMENTO: O programa de biogênese mitocondrial no coração parece apresentar remodelação adaptativa após estresse biomecânico e oxidativo. Os mecanismos adaptativos que protegem o metabolismo do miocárdio durante a hipóxia são coordenados, em parte, pelo óxido nítrico (NO. OBJETIVO: Observar a biogênese mitocondrial e expressão do óxido nítrico sintase (NOS em corações de cardiopatia congênita com cianose; discutir a resposta mitocondrial à hipóxia crônica do miocárdio. MÉTODOS: Foram investigados 20 pacientes com defeitos cardíacos cianóticos (n = 10 ou acianóticos (n = 10. Foram estudadas amostras do miocárdio na via de saída ventricular direita, tomadas durante a operação. A análise morfométrica de mitocôndrias foi realizada por microscopia eletrônica de transmissão. A relação mtDNA/nDNA foi determinada com PCR em tempo real. Os níveis de transcrição da subunidade I da citocromo c oxidase (COXI, coativador-1α do receptor γ ativado por proliferador de peroxissoma (PGC-1α, o fator respiratório nuclear 1 (NRF1, e fator de transcrição mitocondrial A (Tfam foram detectados por reação em cadeia da polimerase via transcriptase reversa (RT-PCR ativado por fluorescência em tempo real. Os níveis proteicos de COXI e nNOS, iNOS e eNOS foram medidos por técnica de Western Blot. RESULTADOS: A densidade volumétrica mitocondrial (Vv e a densidade numérica (Nv foram significativamente elevadas em pacientes com cianose, em comparação com a cardiopatia congênita acianótica. MtDNA elevada e suprarregulação dos níveis de COXI, PGC-1 α, NRF1 e Tfam mRNA foram observadas em pacientes cianóticos. Os níveis de proteína de COXI e eNOS foram significativamente maiores no miocárdio de pacientes cianóticos que nos de acianóticos. Os níveis de transcrição do PGC-1α se correlacionam com os níveis de eNOS. CONCLUSÃO: A biogênese mitocondrial é ativada no miocárdio da via de saída ventricular na

  17. Relationship between endothelial nitric oxide synthase (eNOS) and natural history of intracranial aneurysms: meta-analysis.

    Science.gov (United States)

    Paschoal, Eric Homero Albuquerque; Yamaki, Vitor Nagai; Teixeira, Renan Kleber Costa; Paschoal Junior, Fernando Mendes; Jong-A-Liem, Glaucia Suzanna; Teixeira, Manoel Jacobsen; Yamada, Elizabeth Sumi; Ribeiro-Dos-Santos, Ândrea; Bor-Seng-Shu, Edson

    2018-01-01

    The aneurysmal subarachnoid hemorrhage is a major public health problem described as a sudden drastic event with no warning symptoms and high morbidity and mortality rates. The role of the endothelial isoform of nitric oxide synthase gene polymorphism in intracranial aneurysms (IAs) is still a matter of controversy with divergent findings among European, American, and Asian populations. Our study purposed to test the association between intracranial aneurysms formation and nitric oxide gene polymorphisms through a systematic review and meta-analysis. Systematic search on Medline, Lilacs, and EMBASE was performed. The primary search resulted in 139 papers, out of which 9 met our inclusion criteria after a full text analysis. The dominant T786C model found a significant association with IA (OR 1.22, 95 % CI 1.04-1.44, p = 0.01), so did studies of the recessive T786C model (OR 0.37, 95 % CI 0.30-0.45, p < 0.0001) but with opposite effect. Our findings support the presence of the T786C polymorphism as a predictor for the development of intracranial aneurysm in the cerebral vascular system. More studies are necessary in order to elucidate the pathways of the endothelial nitric oxide synthase (eNOS) in cerebrovascular diseases and in defining how different allelic combinations of the eNOS gene single-nucleotide polymorphism (SNP) could favor this pathological process.

  18. Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation

    Directory of Open Access Journals (Sweden)

    Ho Sub Lee

    2012-01-01

    Full Text Available Vascular inflammation is an important factor which can promote diabetic complications. In this study, the inhibitory effects of aqueous extract from Prunella vulgaris (APV on high glucose (HG-induced expression of cell adhesion molecules in human umbilical vein endothelial cells (HUVEC are reported. APV decreased HG-induced expression of intercellular adhesion molecule-1 (ICAM-1, vascular cell adhesion molecule-1 (VCAM-1, and E-selectin. APV also dose-dependently inhibited HG-induced adhesion of HL-60 monocytic cells. APV suppressed p65 NF-κB activation in HG-treated cells. APV significantly inhibited the formation of intracellular reactive oxygen species (ROS. HG-stimulated HUVEC secreted gelatinases, however, APV inhibited it. APV induced Akt phosphorylation as well as activation of heme oxygenase-1 (HO-1, eNOS, and nuclear factor E2-related factor 2 (Nrf2, which may protect vascular inflammation caused by HG. In conclusion, APV exerts anti-inflammatory effect via inhibition of ROS/NF-κB pathway by inducing HO-1 and eNOS expression mediated by Nrf2, thereby suggesting that Prunella vulgaris may be a possible therapeutic approach to the inhibition of diabetic vascular diseases.

  19. Sildenafil Ameliorates Gentamicin-Induced Nephrotoxicity in Rats: Role of iNOS and eNOS

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    Mohamed A. Morsy

    2014-01-01

    Full Text Available Gentamicin, an aminoglycoside antibiotic, is used for the treatment of serious Gram-negative infections. However, its usefulness is limited by its nephrotoxicity. Sildenafil, a selective phosphodiesterase-5 inhibitor, was reported to prevent or decrease tissue injury. The aim of this study is to evaluate the potential protective effects of sildenafil on gentamicin-induced nephrotoxicity in rats. Male Wistar rats were injected with gentamicin (100 mg/kg/day, i.p. for 6 days with and without sildenafil. Sildenafil administration resulted in nephroprotective effect in gentamicin-intoxicated rats as it significantly decreased serum creatinine and urea, urinary albumin, and renal malondialdehyde and nitrite/nitrate levels, with a concomitant increase in renal catalase and superoxide dismutase activities compared to gentamicin-treated rats. Moreover, immunohistochemical examination revealed that sildenafil treatment markedly reduced inducible nitric oxide synthase (iNOS expression, while expression of endothelial nitric oxide synthase (eNOS was markedly enhanced. The protective effects of sildenafil were verified histopathologically. In conclusion, sildenafil protects rats against gentamicin-induced nephrotoxicity possibly, in part, through its antioxidant activity, inhibition of iNOS expression, and induction of eNOS production.

  20. NOX4-dependent Hydrogen peroxide promotes shear stress-induced SHP2 sulfenylation and eNOS activation.

    Science.gov (United States)

    Sánchez-Gómez, Francisco J; Calvo, Enrique; Bretón-Romero, Rosa; Fierro-Fernández, Marta; Anilkumar, Narayana; Shah, Ajay M; Schröder, Katrin; Brandes, Ralf P; Vázquez, Jesús; Lamas, Santiago

    2015-12-01

    Laminar shear stress (LSS) triggers signals that ultimately result in atheroprotection and vasodilatation. Early responses are related to the activation of specific signaling cascades. We investigated the participation of redox-mediated modifications and in particular the role of hydrogen peroxide (H2O2) in the sulfenylation of redox-sensitive phosphatases. Exposure of vascular endothelial cells to short periods of LSS (12 dyn/cm(2)) resulted in the generation of superoxide radical anion as detected by the formation of 2-hydroxyethidium by HPLC and its subsequent conversion to H2O2, which was corroborated by the increase in the fluorescence of the specific peroxide sensor HyPer. By using biotinylated dimedone we detected increased total protein sulfenylation in the bovine proteome, which was dependent on NADPH oxidase 4 (NOX4)-mediated generation of peroxide. Mass spectrometry analysis allowed us to identify the phosphatase SHP2 as a protein susceptible to sulfenylation under LSS. Given the dependence of FAK activity on SHP2 function, we explored the role of FAK under LSS conditions. FAK activation and subsequent endothelial NO synthase (eNOS) phosphorylation were promoted by LSS and both processes were dependent on NOX4, as demonstrated in lung endothelial cells isolated from NOX4-null mice. These results support the idea that LSS elicits redox-sensitive signal transduction responses involving NOX4-dependent generation of hydrogen peroxide, SHP2 sulfenylation, and ulterior FAK-mediated eNOS activation. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Juba teine "1+1 = 1"

    Index Scriptorium Estoniae

    2004-01-01

    15. III Okupatsioonimuuseumis toimuva "1+1=1" teise ürituse kavast. Presentatsioonide õhtu keskendub konkreetse ruumikogemuse vahendamisele kunstis (helis, slaidi-installatsioonis, tänava-aktsioonis, skulptuuris, videos, filmis). Esinevad Cevdet Erek, Andres Lõo, Andres Kurg (arhitektuur ja interjöörid Marko Raadi filmis "Agent Sinikael") jt.

  2. Teine Res Musica ilmunud / Jaan Ross

    Index Scriptorium Estoniae

    Ross, Jaan, 1957-

    2010-01-01

    Artiklikogumikust: Res musica [2] : Eesti Muusikateaduse Seltsi ja Eesti Muusika- ja Teatriakadeemia muusikateaduse osakonna aastaraamat / peatoimetaja Urve Lippus. Tallinn : Eesti Muusika- ja Teatriakadeemia, 2010

  3. Teine Ameerika / Tristan Priimägi

    Index Scriptorium Estoniae

    Priimägi, Tristan, 1976-

    2007-01-01

    2.-9. veebr. on kinos "Sõprus" ja 11.-15. veebr. Tartu Sadamateatris 5. kultusfilmide festival Manifest, seekord 8 USA filmi aastaist 1946-2006, teemaks sõnavabadus. Mõnest filmist lähemalt ja nende tähendusest ameerika ühiskonnale

  4. Probleemid on samad, taust teine / Karl Kello

    Index Scriptorium Estoniae

    Kello, Karl, 1950-

    2010-01-01

    Vene- ja eesti kooli probleemid on ühesugused, mured õpetajakoolituse, õpilaste arvu vähenemise, õppekavadega. Vaja oleks venekeelseid õpetajakoolitusi. Vestlusest Tallinna kesklinna vene gümnaasiumi direktori Sergei Teploviga

  5. Kunstisuvi 2008, teine osa / Andri Ksenofontov

    Index Scriptorium Estoniae

    Ksenofontov, Andri, 1962-

    2008-01-01

    Sirje Runge tööde näitus Eesti Panga galeriis. Aneri Kärkkäineni ja Elis Saarevälja näitus "Sad Sack" ArtDepoo galeriis. Nadezhda Tshernobai "Kollase süütuse suudlus", Ilme Rätsepa kangamaalid "Kolmainsus" ning Kadri Kangilaski ja Toomas Tõnissoo maalinäitus "Perekonnaelu" Draakoni galeriis. Kati Kerstna ja Kairi Orgusaare "Läbivaade" Tallinna Kunstihoone galeriis. Mare Saare "Fragiilid" ja Alice Kase tööd Hobusepea galeriis. Ulvi Haagenseni "Antipoodid" HOP galeriis. Alvar Reisneri "Extra & Ordinary" Vaal-galeriis. Liisi Eelmaa videoinstallatsioon-aktsioon "Õnnekeeks" (11. VII), Minna Hindi interaktiivne installatsioon "Ainult täna õhtul: Minna Hint" (18. VII), Sirli Heina maal "Kirsitort" (16. VII) Artishoki näitusel Tallinna Linnagaleriis. Ado Lille "Nude alive" Haus galeriis. Toomas Altnurme "Metafüüsika seitsmes dimensioon" SEBi galeriis. 5. VII toimunud kunstinädala "Kultuurikatapuldid 2008" üritused (esinejad: Linnar Priimägi, Jüri Ojaver, Erik Alalooga, Siim-Tanel Annus, E. Saareväli, Jaan Toomik, Kiwa) Haapsalus

  6. Enolase 1 (ENO1 and protein disulfide-isomerase associated 3 (PDIA3 regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells

    Directory of Open Access Journals (Sweden)

    Kathrin Mutze

    2015-08-01

    Full Text Available The alveolar epithelium represents a major site of tissue destruction during lung injury. It consists of alveolar epithelial type I (ATI and type II (ATII cells. ATII cells are capable of self-renewal and exert progenitor function for ATI cells upon alveolar epithelial injury. Cell differentiation pathways enabling this plasticity and allowing for proper repair, however, are poorly understood. Here, we applied proteomics, expression analysis and functional studies in primary murine ATII cells to identify proteins and molecular mechanisms involved in alveolar epithelial plasticity. Mass spectrometry of cultured ATII cells revealed a reduction of carbonyl reductase 2 (CBR2 and an increase in enolase 1 (ENO1 and protein disulfide-isomerase associated 3 (PDIA3 protein expression during ATII-to-ATI cell trans-differentiation. This was accompanied by increased Wnt/β-catenin signaling, as analyzed by qRT-PCR and immunoblotting. Notably, ENO1 and PDIA3, along with T1α (podoplanin; an ATI cell marker, exhibited decreased protein expression upon pharmacological and molecular Wnt/β-catenin inhibition in cultured ATII cells, whereas CBR2 levels were stabilized. Moreover, we analyzed primary ATII cells from mice with bleomycin-induced lung injury, a model exhibiting activated Wnt/β-catenin signaling in vivo. We observed reduced CBR2 significantly correlating with surfactant protein C (SFTPC, whereas ENO1 and PDIA3 along with T1α were increased in injured ATII cells. Finally, siRNA-mediated knockdown of ENO1, as well as PDIA3, in primary ATII cells led to reduced T1α expression, indicating diminished cell trans-differentiation. Our data thus identified proteins involved in ATII-to-ATI cell trans-differentiation and suggest a Wnt/β-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair.

  7. Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency.

    Science.gov (United States)

    Fallahi, Ali Asghar; Shekarfroush, Shahnaz; Rahimi, Mostafa; Jalali, Amirhossain; Khoshbaten, Ali

    2016-03-01

    High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Wistar rats were divided into five groups: control group (n=12), HIIT for an acute bout (AT1), short term HIIT for 3 and 5 sessions (ST3 and ST5), long-term training for 8 weeks (LT) (6 in each group). The rats of the training groups were made to run on a treadmill for 60 min in three stages: 6 min running for warm-up, 7 intervals of 7 min running on treadmill with a slope of 5° to 20° (4 min with an intensity of 80-110% VO2max and 3 min at 50-60% VO2max), and 5-min running for cool-down. The control group did not participate in any exercise program. Rats were sacrificed and the hearts were extracted to analyze the levels of UCP2, UCP3 and eNOS mRNA by RT-PCR. UCP3 expression was increased significantly following an acute training bout. Repeated HIIT for 8 weeks resulted in a significant decrease in UCPs mRNA and a significant increase in eNOS expression in cardiac muscle. This study indicates that Long term HIIT through decreasing UCPs mRNA and increasing eNOS mRNA expression may enhance energy efficiency and physical performance.

  8. Eno-culture and Identity: The installation as a new methodology in Artistic Research and Heritage Education

    Directory of Open Access Journals (Sweden)

    Ruth Marañón Martínez de la Puente

    2018-03-01

    Full Text Available The aesthetic experience and the artistic knowledge always have been connected with the Heritage Education, due to the great importance in rewarding pedagogic processes, specially, in non-formal spaces. In our research, the installation (with its features and competences has turned into the most interesting educational tool to transform the reality of our work’s context: Rioja Alavesa. Thus, across the workshops "Instálate" (organized inside different wineries of the region, we worked the Wine Cultural Heritage (Eno-culture, the Artistic Research and the knowledge based on the sensitive experience. In this article, we showed the results of this experience, proposing the installation as a new interesting and practical method of Artistic Research in Heritage Education. In conclusion, we propose the art-installation as a different key to work the identity, contemporary art, culture and heritage.

  9. Chronic aerobic exercise associated to dietary modification improve endothelial function and eNOS expression in high fat fed hamsters.

    Directory of Open Access Journals (Sweden)

    Beatriz C S Boa

    Full Text Available Obesity is epidemic in the western world and central adipose tissue deposition points to increased cardiovascular morbidity and mortality, independently of any association between obesity and other cardiovascular risk factors. Physical exercise has been used as non-pharmacological treatment to significantly reverse/attenuate obesity comorbidities. In this study we have investigated effects of exercise and/or dietary modification on microcirculatory function, body composition, serum glucose, iNOS and eNOS expression on 120 male hamsters treated for 12 weeks with high fat chow (HF, n = 30 starting on the 21st day of birth. From week 12 to 20, animals were randomly separated in HF (no treatment change, return to standard chow (HFSC, n = 30, high fat chow associated to an aerobic exercise training program (AET (HFEX, n = 30 and return to standard chow+AET (HFSCEX, n = 30. Microvascular reactivity in response to acetylcholine and sodium nitroprusside and macromolecular permeability increase induced by 30 minutes ischemia followed by reperfusion were assessed on the cheek pouch preparation. Total body fat and aorta eNOS and iNOS expression by immunoblotting assay were evaluated on the experimental day. Compared to HFSC and HFSCEX groups, HF and HFEX ones presented increased visceral fat [(mean±SEM (HF4.9±1.5 g and (HFEX4.7±0.9 g vs. (HFSC*3.0±0.7 g and (HFSCEX*1.9±0.4 g/100 g BW]; impaired endothelial-dependent vasodilatation [Ach 10(-8 M (HF87.9±2.7%; (HFSC*116.7±5.9%; (HFEX*109.1±4.6%; (HFSCEX*105±2.8%; Ach10(-6 M (HF95.3±3.1%; (HFSC*126±6.2%; (HFEX*122.5±2.8%; (HFSCEX*118.1±4.3% and Ach10(-4 M (HF109.5±4.8%; (HFSC*149.6±6.6%; (HFEX*143.5±5.4% and (HFSCEX*139.4±5.2%], macromolecular permeability increase after ischemia/reperfusion [(HF40.5±4.2; (HFSC*19.0±1.6; (HFEX*18.6±2.1 and (HFSCEX* 21.5±3.7 leaks/cm2, decreased eNOS expression, increased leptin and glycaemic levels. Endothelial-independent microvascular

  10. A Multilevel Prediction of Physiological Response to Challenge: Interactions among Child Maltreatment, Neighborhood Crime, eNOS and GABRA6

    Science.gov (United States)

    Lynch, Michael; Manly, Jody Todd; Cicchetti, Dante

    2015-01-01

    Physiological response to stress has been linked to variety of healthy and pathological conditions. The current study conducted a multilevel examination of interactions among environmental toxins – i.e., neighborhood crime and child maltreatment – and specific genetic polymorphisms of eNOS and GABRA6. A total of 186 children were recruited at age 4. At this time, the presence or absence of child maltreatment was determined, as was the amount of crime that occurred in their neighborhood during the previous year. At age 9, the children were brought to the lab where their physiological response to a cognitive challenge – i.e., change in the amplitude of RSA – was assessed and DNA samples were collected for subsequent genotyping. Results confirmed that complex G x G, E x E, and G x E interactions were associated with different patterns of RSA reactivity. The implications for future research and evidence-based intervention are discussed. PMID:26535938

  11. Effects of aerobic exercise on the blood pressure, oxidative stress and eNOS gene polymorphism in pre-hypertensive older people.

    Science.gov (United States)

    Zago, Anderson Saranz; Park, Joon-Young; Fenty-Stewart, Nicola; Silveira, Leonardo Reis; Kokubun, Eduardo; Brown, Michael D

    2010-11-01

    The polymorphisms of endothelial nitric oxide synthase (eNOS) are associated with reduced eNOS activity. Aerobic exercise training (AEX) may influence resting nitric oxide (NO) production, oxidative stress and blood pressure. The purpose of this study was to investigate the effect of AEX on the relationship among blood pressure, eNOS gene polymorphism and oxidative stress in pre-hypertensive older people. 118 pre-hypertensive subjects (59 ± 6 years) had blood samples collected after a 12 h overnight fast for assessing plasma NO metabolites (NOx) assays, thiobarbituric acid reactive substances (T-BARS) and superoxide dismutase activity (ecSOD). eNOS polymorphism (T-786C and G-894T) was done by standard PCR methods. All people were divided according to the genotype results (G1: TT/GG, G2: TT/GT + TT, G3: TC + CC/GG, G4: TC + CC/GT + TT). All parameters were measured before and after 6 months of AEX (70% of VO(2 max)). At baseline, no difference was found in systolic and diastolic blood pressure, ecSOD and T-BARS activity. Plasma NOx levels were significantly different between G1 (19 ± 1 μM) and G4 (14.2 ± 0.6 μM) and between G2 (20.1 ± 1.7 μM) and G4 (14.2 ± 0.6 μM). Therefore, reduced NOx concentration in G4 group occurred only when the polymorphisms were associated, suggesting that these results are more related to genetic factors than NO-scavenging effect. After AEX, the G4 increased NOx values (17.2 ± 1.2 μM) and decreased blood pressure. G1, G3 and G4 decreased T-BARS levels. These results suggest the AEX can modulate the NOx concentration, eNOS activity and the relationship among eNOS gene polymorphism, oxidative stress and blood pressure especially in C (T-786C) and T (G-894T) allele carriers.

  12. Evaluation of Protective Immune Responses Induced by Recombinant TrxLp and ENO2 Proteins against Toxoplasma gondii Infection in BALB/c Mice

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    Meng Wang

    2016-01-01

    Full Text Available Toxoplasma gondii is an obligate intracellular parasitic protozoan that can infect almost all species of warm-blooded animals. As any chemical-based drugs could not act against the tissue cyst stage of T. gondii, vaccination may be one of the ideal control strategies. In the present study, two new vaccine candidates, named TgENO2 and TgTrxLp, were purified from Escherichia coli with pET-30a(+ expression system and then were injected into BALB/c mice to evaluate the protective efficacy against acute and chronic toxoplasmosis. The results showed that both the recombinant proteins, either alone or in combination, could elicit strong humoral and cellular immune responses with a higher level of IgG antibodies, IFN-γ, IL-2, CD4+, and CD8+ T cells as compared to those in mice from control groups. After acute challenge with tachyzoites of the GJS strain, mice immunized with rTgTrxLp (8±2.77 d, rTgENO2 (7.4±1.81 d, and rTgTrxLp + rTgENO2 (8.38±4.57 d proteins showed significantly longer survival time than those that received Freund’s adjuvant (6.78±2.08 d and PBS (6.38±4.65 d (χ2 = 9.687, df = 4, P=0.046. The protective immunity of rTgTrxLp, rTgENO2, and rTgTrxLp + rTgENO2 proteins against chronic T. gondii infection showed 69.77%, 58.14%, and 20.93% brain cyst reduction as compared to mice that received PBS. The present study suggested that both TgENO2 and TgTrxLp were potential candidates for the development of multicomponent vaccines against toxoplasmosis.

  13. Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery

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    Luciana N. Moreira

    2018-05-01

    Full Text Available D-pinitol is a cyclitol present in several edible plant species and extensively investigated for the treatment of metabolic diseases in humans, as food supplement, and demonstrated protective effects in the cardiovascular system. For these reasons, the present work aimed at investigating the mechanisms involved in the vascular effects of D-pinitol in mouse mesenteric artery. Mesenteric arteries from male C57BL/6 mice were mounted in a wire myograph. Nitrite was measured by the 2,3-diaminonaphthalene (DAN method. Protein expression and phosphorylation were measured by Western blot. The systolic blood pressure (SBP was measured by tail-cuff plethysmography. D-pinitol induced a concentration-dependent vasodilatation in endothelium-intact, but not in endothelium-denuded arteries. Nω-Nitro-L-arginine methyl ester (300 μM abolished the effect of D-pinitol, while 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 μM shifted the concentration-response curve to the right. KN-93 (1 μM blunted the vasodilator effect of D-pinitol, but H-89 (0.1 μM did not change it. 1-[2-(Trifluoromethyl phenyl]imidazole (300 μM, indomethacin (10 μM, celecoxib (5 μM, wortmannin (1 μM, ruthenium red (10 μM, tiron (10 μM, MnTMPyP (30 μM, MPP (0.1 μM, PHTPP (0.1 μM, and atropine (1 μM did not change the effect of D-pinitol. D-pinitol increased the concentration of nitrite, which was inhibited by L-NAME and calmidazolium (10 μM. D-pinitol increased the phosphorylation level of eNOS activation site at Ser1177 and reduced the phosphorylation level of its inactivation site at Thr495. In normotensive mice, the intraperitoneal administration of D-pinitol (10 mg/kg induced a significant reduction of the SBP after 30 min. The present results led us to conclude that D-pinitol has an endothelium- and NO-dependent vasodilator effect in mouse mesenteric artery through a mechanism dependent on the activation of eNOS by the calcium-calmodulin complex, which can explain its

  14. Variable effects of maternal and paternal-fetal contribution to the risk for preeclampsia combining GSTP1, eNOS, and LPL gene polymorphisms.

    Science.gov (United States)

    Pappa, Kalliopi I; Roubelakis, Maria; Vlachos, George; Marinopoulos, Spyros; Zissou, Antonia; Anagnou, Nicholas P; Antsaklis, Aris

    2011-04-01

    To evaluate the maternal, paternal, and fetal genotype contribution to preeclampsia. STUDY DESIGN, MATERIALS, AND METHODS: We combined the analysis of polymorphisms of the GSTP1, eNOS, and LPL genes - affecting biotransformation enzymes and endothelial function - in a cohort of 167 preeclamptic and normal control trios (mother, father, and child) comprising a total of 501 samples in the Greek population, never analyzed before by this approach. For the frequency of the GSTP1 Ile(105)/Val(105), the eNOS Glu298Asp and the LPL-93 polymorphisms, statistically significant differences were found between the two groups. However, the transmission rates of the parental alleles to neonates studied by the transmission disequilibrium test, disclosed no increased rate of transmission to preeclampsia children for the variant alleles of Val(105) GSTP1, 298Asp eNOS, and -93G LPL. These novel data, suggest that interaction of all three types of genotypes (mother, father and neonate), reveals no effects on the development of preeclampsia, but provide the impetus for further studies to decipher the individual contribution of each genetic parameter of preeclampsia.

  15. Exercise Training Could Improve Age-Related Changes in Cerebral Blood Flow and Capillary Vascularity through the Upregulation of VEGF and eNOS

    Directory of Open Access Journals (Sweden)

    Sheepsumon Viboolvorakul

    2014-01-01

    Full Text Available This study aimed to investigate the effect of exercise training on age-induced microvascular alterations in the brain. Additionally, the association with the protein levels of vascular endothelial growth factor (VEGF and endothelial nitric oxide synthase (eNOS was also assessed. Male Wistar rats were divided into four groups: sedentary-young (SE-Young, n=5, sedentary aged (SE-Aged, n=8, immersed-aged (IM-Aged, n=5, and exercise trained-aged (ET-Aged, 60 minutes/day and 5 days/week for 8 weeks, n=8 rats. The MAPs of all aged groups, SE-Aged, IM-Aged, and ET-Aged, were significantly higher than that of the SE-Young group. The regional cerebral blood flow (rCBF in the SE-Aged and IM-Aged was significantly decreased as compared to SE-Young groups. However, rCBF of ET-Aged group was significantly higher than that in the IM-Aged group (P<0.05. Moreover, the percentage of capillary vascularity (%CV and the levels of VEGF and eNOS in the ET-Aged group were significantly increased compared to the IM-Aged group (P<0.05. These results imply that exercise training could improve age-induced microvascular changes and hypoperfusion closely associated with the upregulation of VEGF and eNOS.

  16. Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro

    Directory of Open Access Journals (Sweden)

    Jun-Xiu Liu

    2014-05-01

    Full Text Available Angiogenesis is the formation of blood vessels from pre-existing vasculature. Excessive or uncontrolled angiogenesis is a major contributor to many pathological conditions whereas inhibition of aberrant angiogenesis is beneficial to patients with pathological angiogenesis. Catunaregin is a core of novel marine compound isolated from mangrove associate. The potential anti-angiogenesis of catunaregin was investigated in human umbilical vein endothelial cells (HUVECs and zebrafish. HUVECs were treated with different concentrations of catunaregin in the presence or absence of VEGF. The angiogenic phenotypes including cell invasion cell migration and tube formation were evaluated following catunaregin treatment in HUVECs. The possible involvement of AKT, eNOS and ERK1/2 in catunaregin-induced anti-angiogenesis was explored using Western blotting. The anti-angiogenesis of catunaregin was further tested in the zebrafish embryo neovascularization and caudal fin regeneration assays. We found that catunaregin dose-dependently inhibited angiogenesis in both HUVECs and zebrafish embryo neovascularization and zebrafish caudal fin regeneration assays. In addition, catunaregin significantly decreased the phosphorylation of Akt and eNOS, but not the phosphorylation of ERK1/2. The present work demonstrates that catunaregin exerts the anti-angiogenic activity at least in part through the regulation of the Akt and eNOS signaling pathways.

  17. Expression profiles of eNOS, iNOS and microRNA-27b in the corpus cavernosum of rats submitted to chronic alcoholism and Diabetes mellitus.

    Science.gov (United States)

    Cunha, Joao Paulo da; Lizarte, Fermino Sanches; Novais, Paulo Cezar; Gattas, Daniela; Carvalho, Camila Albuquerque Mello de; Tirapelli, Daniela Pretti da Cunha; Molina, Carlos Augusto Fernandes; Tirapelli, Luis Fernando; Tucci, Silvio

    2017-01-01

    To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies. Forty eight Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood. Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups. The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.

  18. Glycolipids from spinach suppress LPS-induced vascular inflammation through eNOS and NK-κB signaling.

    Science.gov (United States)

    Ishii, Masakazu; Nakahara, Tatsuo; Araho, Daisuke; Murakami, Juri; Nishimura, Masahiro

    2017-07-01

    Glycolipids are the major constituent of the thylakoid membrane of higher plants and have a variety of biological and pharmacological activities. However, anti-inflammatory effects of glycolipids on vascular endothelial cells have not been elucidated. Here, we investigated the effect of glycolipids extracted from spinach on lipopolysaccharides (LPS)-induced endothelial inflammation and evaluated the underlying molecular mechanisms. Treatment with glycolipids from spinach had no cytotoxic effects on cultured human umbilical vein endothelial cells (HUVECs) and significantly blocked the expression of LPS-induced interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1), and intracellular adhesion molecule-1 (ICAM-1) in them. Glycolipids treatment also effectively suppressed monocyte adhesion to HUVECs. Treatment with glycolipids inhibited LPS-induced NF-κB phosphorylation and nuclear translocation. In addition, glycolipids treatment significantly promoted endothelial nitric oxide synthase (eNOS) activation and nitric oxide (NO) production in HUVECs. Furthermore, glycolipids treatment blocked LPS-induced inducible NOS (iNOS) expression in HUVECs. Pretreatment with a NOS inhibitor attenuated glycolipids-induced suppression of NF-κB activation and adhesion molecule expression, and abolished the glycolipids-mediated suppression of monocyte adhesion to HUVECs. These results indicate that glycolipids suppress LPS-induced vascular inflammation through attenuation of the NF-κB pathway by increasing NO production in endothelial cells. These findings suggest that glycolipids from spinach may have a potential therapeutic use for inflammatory vascular diseases. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  19. The effect of high protein diet and exercise on irisin, eNOS, and iNOS expressions in kidney.

    Science.gov (United States)

    Tastekin, Ebru; Palabiyik, Orkide; Ulucam, Enis; Uzgur, Selda; Karaca, Aziz; Vardar, Selma Arzu; Yilmaz, Ali; Aydogdu, Nurettin

    2016-08-01

    Long-term effects of high protein diets (HPDs) on kidneys are still not sufficiently studied. Irisin which increases oxygen consumption and thermogenesis in white fat cells was shown in skeletal muscles and many tissues. Nitric oxide synthases (NOS) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. We aimed to investigate the effects of HPD, irisin and NO expression in kidney and relation of them with exercise and among themselves. Animals were grouped as control, exercise, HPD and exercise combined with HPD (exercise-HPD). Rats were kept on a HPD for 5 weeks and an exercise program was given them as 5 exercise and 2 rest days per week exercising on a treadmill with increasing speed and angle. In our study, while HPD group had similar total antioxidant capacity (TAC) levels with control group, exercise and exercise-HPD groups had lower levels (p highly selective and quite clear in this study. Effects of exercise and HPD on kidney should be evaluated with different exercise protocols and contents of the diet. İrisin, eNOS, and iNOS staining localizations should be supported with various research studies.

  20. MiR-21 is induced in endothelial cells by shear stress and modulates apoptosis and eNOS activity

    Energy Technology Data Exchange (ETDEWEB)

    Weber, Martina; Baker, Meredith B.; Moore, Jeffrey P. [Division of Cardiology, Emory University, 1639 Pierce Drive, WMB 319, Atlanta, GA 30322 (United States); Searles, Charles D., E-mail: csearle@emory.edu [Division of Cardiology, Emory University, 1639 Pierce Drive, WMB 319, Atlanta, GA 30322 (United States); Atlanta Veterans Administration Medical Center, 1670 Clarimont Road, Decatur, GA 30033 (United States)

    2010-03-19

    Mechanical forces associated with blood flow play an important role in regulating vascular signaling and gene expression in endothelial cells (ECs). MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. miRNAs are known to have an important role in modulating EC biology, but their expression and functions in cells subjected to shear stress conditions are unknown. We sought to determine the miRNA expression profile in human ECs subjected to unidirectional shear stress and define the role of miR-21 in shear stress-induced changes in EC function. TLDA array and qRT-PCR analysis performed on HUVECs exposed to prolonged unidirectional shear stress (USS, 24 h, 15 dynes/cm{sup 2}) identified 13 miRNAs whose expression was significantly upregulated (p < 0.05). The miRNA with the greatest change was miR-21; it was increased 5.2-fold (p = 0.002) in USS-treated versus control cells. Western analysis demonstrated that PTEN, a known target of miR-21, was downregulated in HUVECs exposed to USS or transfected with pre-miR-21. Importantly, HUVECs overexpressing miR-21 had decreased apoptosis and increased eNOS phosphorylation and nitric oxide (NO{sup {center_dot}}) production. These data demonstrate that shear stress forces regulate the expression of miRNAs in ECs, and that miR-21 influences endothelial biology by decreasing apoptosis and activating the NO{sup {center_dot}} pathway. These studies advance our understanding of the mechanisms by which shear stress forces modulate vascular homeostasis.

  1. Ibuprofen arginate retains eNOS substrate activity and reverses endothelial dysfunction: implications for the COX-2/ADMA axis.

    Science.gov (United States)

    Kirkby, Nicholas S; Tesfai, Abel; Ahmetaj-Shala, Blerina; Gashaw, Hime H; Sampaio, Walkyria; Etelvino, Gisele; Leão, Nádia Miricéia; Santos, Robson A; Mitchell, Jane A

    2016-12-01

    Nonsteroidal antiinflammatory drugs, including ibuprofen, are among the most commonly used medications and produce their antiinflammatory effects by blocking cyclooxygenase (COX)-2. Their use is associated with increased risk of heart attacks caused by blocking COX-2 in the vasculature and/or kidney, with our recent work implicating the endogenous NOS inhibitor asymmetric dimethylarginine (ADMA), a cardiotoxic hormone whose effects can be prevented by l-arginine. The ibuprofen salt ibuprofen arginate (Spididol) was created to increase solubility but we suggest that it could also augment the NO pathway through codelivery of arginine. Here we investigated the idea that ibuprofen arginate can act to simultaneously inhibit COX-2 and preserve the NO pathway. Ibuprofen arginate functioned similarly to ibuprofen sodium for inhibition of mouse/human COX-2, but only ibuprofen arginate served as a substrate for NOS. Ibuprofen arginate but not ibuprofen sodium also reversed the inhibitory effects of ADMA and N G -nitro-l-arginine methyl ester on inducible NOS (macrophages) and endothelial NOS in vitro (aorta) and in vivo (blood pressure). These observations show that ibuprofen arginate provides, in one preparation, a COX-2 inhibitor and NOS substrate that could act to negate the harmful cardiovascular consequences mediated by blocking renal COX-2 and increased ADMA. While remarkably simple, our findings are potentially game-changing in the nonsteroidal antiinflammatory drug arena.-Kirkby, N. S., Tesfai, A., Ahmetaj-Shala, B., Gashaw, H. H., Sampaio, W., Etelvino, G., Leão, N. M., Santos, R. A., Mitchell, J. A. Ibuprofen arginate retains eNOS substrate activity and reverses endothelial dysfunction: implications for the COX-2/ADMA axis. © The Author(s).

  2. Arginase up-regulation and eNOS uncoupling contribute to impaired endothelium-dependent vasodilation in a rat model of intrauterine growth restriction.

    Science.gov (United States)

    Grandvuillemin, Isabelle; Buffat, Christophe; Boubred, Farid; Lamy, Edouard; Fromonot, Julien; Charpiot, Philippe; Simoncini, Stephanie; Sabatier, Florence; Dignat-George, Françoise; Peyter, Anne-Christine; Simeoni, Umberto; Yzydorczyk, Catherine

    2018-05-09

    Individuals born after intrauterine growth restriction (IUGR) are at increased risk of developing cardiovascular diseases in adulthood, notably hypertension (HTN). Alterations in the vascular system, particularly impaired endothelium-dependent vasodilation, may play an important role in long-term effects of IUGR. Whether such vascular dysfunction precedes HTN has not been fully established in individuals born after IUGR. Moreover, the intimate mechanisms of altered endothelium-dependent vasodilation remain incompletely elucidated. We therefore investigated, using a rat model of IUGR, whether impaired endothelium-dependent relaxation precedes the development of HTN and whether key components of the L-Arginine-nitric oxide (NO) pathway are involved in its pathogenesis. Pregnant rats were fed with a control (CTRL, 23% casein) or low-protein diet (LP, 9% casein) to induce IUGR. Systolic blood pressure (SBP) was measured by tail-cuff plethysmography in 5- and 8-week-old male offspring. Aortic rings were isolated to investigate relaxation to acetylcholine, NO production, eNOS protein content, arginase activity, and superoxide anion production. SBP was not different at 5 weeks, but significantly increased in 8-week-old LP vs. CRTL offspring. In 5-week-old LP vs. CRTL males, endothelium-dependent vasorelaxation was significantly impaired, but restored by pre-incubation with L-Arginine or the arginase inhibitor BEC; NO production was significantly reduced, but restored by L-Arginine pretreatment; total eNOS protein, dimer/monomer ratio, and arginase activity were significantly increased; superoxide anion production was significantly enhanced, but normalized by pretreatment with the NOS inhibitor L-NNA. In this model, IUGR leads to early-impaired endothelium-dependent vasorelaxation, resulting from arginase up-regulation and eNOS uncoupling, which precedes the development of HTN.

  3. Enhanced estradiol-induced vasorelaxation in aortas from type 2 diabetic mice may reflect a compensatory role of p38 MAPK-mediated eNOS activation.

    Science.gov (United States)

    Taguchi, Kumiko; Morishige, Akitaka; Matsumoto, Takayuki; Kamata, Katsuo; Kobayashi, Tsuneo

    2012-08-01

    Cardiovascular problems are a major cause of morbidity and mortality, mainly due to coronary artery disease and atherosclerosis, in type 2 diabetes mellitus. However, female gender is a protective factor in the development of, for example, atherosclerosis and hypertension. One of the female hormones, 17β-estradiol (E2), is known to protect against the cardiovascular injury resulting from endothelial dysfunction, but the mechanism by which it does so remains unknown. Our hypothesis was that E2-mediated activation of Akt and mitogen-activated protein kinase (MAPK), and the subsequent endothelial NO synthase (eNOS) phosphorylation, might protect the aorta in diabetic mellitus. The experimental type 2 diabetic model we employed to test that hypothesis (female mice given streptozotocin and nicotinamide) is here termed fDM. In fDM aortas, we examined the E2-induced relaxation response and the associated protein activities. In control (age-matched, nondiabetic) aortas, E2 induced a vascular relaxation response that was mediated via Akt/eNOS and mitogen-activated/ERK-activating kinase (MEK)/eNOS pathways. In fDM aortas (vs. control aortas), (a) the E2-induced relaxation was enhanced, (b) the mediation of the response was different (via Akt/eNOS and p38 MAPK/eNOS pathways), and (c) E2 stimulation increased p38 MAPK and eNOS phosphorylations, decreased MEK phosphorylation, but did not alter estrogen receptor activity. We infer that at least in fDM aortas, E2 has beneficial effects (enhanced vascular relaxation and protection) that are mediated through Akt activation and (compensating for reduced MEK activation) p38 MAPK activation, leading to enhanced eNOS phosphorylation.

  4. Influence of eNOS gene 4a/bVNTR polymorphism on development of endothelial dysfunction and respiratory system disorders in children - residents of radioactively contaminated areas

    International Nuclear Information System (INIS)

    Stepanova, Je.Yi.; Kolpakov, Yi.Je.; Zigalo, V.M.; And Others

    2015-01-01

    Total of 184 children were examined to determine the possible associative links between eNOS gene intron 4m polymorphism and indices characterizing the functional state of the endothelium and the bronchopulmonary system. Of them the main group of children consisted of 135 childrenresidents of radioactively contaminated areas, the control group - of 49 healthy children who lived in ''clean'' regions by the radioactive con tamination and were not belonging to victims of the Chernobyl accident contingents. The molecular genetic study determining the eNOS gene intron 4 polymorphism was performed. Children of the main group did not differ from that of control and literature data by the frequency of geno types 4a/b and eNOS gene allele polymorphism. An increase in the duration of circulation restoration up to the ini tial level after occlusion test was noted in children of the main group with genotype 4a/4b comparing to children with genotype 4b/4b ; a decrease in the nitrite content was observed in blood serum. The integral indices of elastic ity and tensility of the lung tissue, respiratory permeability were decreased; the signs of bronchospasm were record ed 1.5 times more often. These adverse effects were associated with the presence of genotype allele 4a , the signs of endothelial dysfunction and the level of the incorporated 137 Cs. More pronounced changes in vascular endothelial reaction to occlusion and the significant decrease of serum nitrite level in allele 4a carriers, especially in children who live in adverse ecological conditions, give the right to include them into the risk group for the development of endothelial dysfunction, and to conside pulmonary dysfunction as one of its manifestations

  5. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus O.; Mortensen, Alicja; Nilas, Lisbeth

    2007-01-01

    Objectives: To evaluate the effect of estrogen replacement therapy or soy isoflavones supplement on endothelium-dependent relaxation in vitro and gene expression of endothelial nitric oxide synthase (eNOS) in cerebral arteries in a rabbit model of human hypercholesterolemia. Study design: Thirty...... cholesterol was significantly higher at termination in the SoyLife(R) group (P lipoprotein (LDL) cholesterol was comparable in all treatment groups. Neither treatment influenced the endothelium-dependent responses to carbamylcholine chloride or L-NAME or the endothelium...

  6. Influencia del fenómeno ENOS (El Niño y La Niña) en la disponibilidad de grados-día

    OpenAIRE

    Lozada García, Beatriz; Prela, Angélica; Sentelhas, Paulo

    2004-01-01

    Se evaluó la influencia del fenómeno ENOS (El Niño y La Niña) sobre la disponibilidad de calor, en 8 localidades, ubicadas en la región centro-occidental de Venezuela, utilizando concepto de grados-día (GD). Los cambios de la temperatura, representados por las variaciones en los GD, afectarían directamente el ciclo fenológico los cultivos, generando un incremento o reducción en función de desvíos positivos o negativos, respectivamente. Los GD estimados fueron agrupados según el evento predomi...

  7. Relationship of the MTHFD1 (rs2236225, eNOS (rs1799983, CBS (rs2850144 and ACE (rs4343 gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects

    Directory of Open Access Journals (Sweden)

    Mehri Khatami

    2017-09-01

    Full Text Available Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case–control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively. The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD.

  8. Relationship of the MTHFD1 (rs2236225), eNOS (rs1799983), CBS (rs2850144) and ACE (rs4343) gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects.

    Science.gov (United States)

    Khatami, Mehri; Ratki, Farzaneh Morteza; Tajfar, Saba; Akrami, Fatemeh

    2017-09-01

    Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs) in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case-control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively). The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD. Copyright © 2017. Published by Elsevier Taiwan.

  9. Redox-sensitive up-regulation of eNOS by purple grape juice in endothelial cells: role of PI3-kinase/Akt, p38 MAPK, JNK, FoxO1 and FoxO3a.

    Directory of Open Access Journals (Sweden)

    Mahmoud Alhosin

    Full Text Available The vascular protective effect of grape-derived polyphenols has been attributable, in part, to their direct action on blood vessels by stimulating the endothelial formation of nitric oxide (NO. The aim of the present study was to determine whether Concord grape juice (CGJ, which contains high levels of polyphenols, stimulates the expression of endothelial NO synthase (eNOS in porcine coronary artery endothelial cells and, if so, to determine the signaling pathway involved. CGJ dose- and time-dependently increased eNOS mRNA and protein levels and this effect is associated with an increased formation of NO in endothelial cells. The stimulatory effect of CGJ on eNOS mRNA is not associated with an increased eNOS mRNA stability and inhibited by antioxidants such as MnTMPyP, PEG-catalase, and catalase, and by wortmannin (an inhibitor of PI3-kinase, SB 203580 (an inhibitor of p38 MAPK, and SP 600125 (an inhibitor of JNK. Moreover, CGJ induced the formation of reactive oxygen species (ROS in endothelial cells and this effect is inhibited by MnTMPyP, PEG-catalase, and catalase. The CGJ-induced the phosphorylation of p38 MAPK and JNK kinases is abolished by MnTMPyP. CGJ induced phosphorylation of transcription factors FoxO1 and FoxO3a, which regulate negatively eNOS expression, and this effect is prevented by MnTMPyP, PEG-catalase, wortmannin, SB203580 and SP600125. Moreover, chromatin immunoprecipitation assay indicated that the FoxO3a protein is associated with the eNOS promoter in control cells and that CGJ induced its dissociation. Thus, the present study indicates that CGJ up-regulates the expression of eNOS mRNA and protein leading to an increased formation of NO in endothelial cells. The stimulatory effect of CGJ is a redox-sensitive event involving PI3-kinase/Akt, p38 MAPK and JNK pathways, and the inactivation of the FoxO transcription factors, FoxO1 and FoxO3a, thereby preventing their repression of the eNOS gene.

  10. eNOS Glu298Asp polymorphism is associated with development of complicated plaques in patients from Serbia with advanced carotid atherosclerosis

    Directory of Open Access Journals (Sweden)

    Đurić Tamara

    2013-01-01

    Full Text Available Nitric oxide inhibits adhesion of thrombocytes, proliferation and migration of smooth muscle cells and restricts oxidation of atherogenic low-density lipoproteins. Therefore, decreased production or activity of NO may play a role in the initiation, progression or complications of atherosclerosis. The aim of this study was to estimate the effect of Glu298Asp eNOS gene polymorphism on the individual risk for development of complicated carotid atherosclerotic plaque in patients from Serbia with advanced carotid atherosclerosis (CA who had undergone endarterectomy. The study population included 233 patients. eNOS G894T gene polymorphism was identified by PCR and RFLP methods. Multivariate logistic regression analysis showed that Asp298Asp is an independent risk factor for the presence of complicated plaques in CA patients. Patients who were homozygous for the Asp298 allele had an adjusted OR of 4.36 for the development of complicated plaques compared to those that carry the Glu298 allele. Further validation and replication studies are needed. [Projekat Ministarstva nauke Republike Srbije, br. OI175085

  11. Vascular endothelial dysfunction in β-thalassemia occurs despite increased eNOS expression and preserved vascular smooth muscle cell reactivity to NO.

    Directory of Open Access Journals (Sweden)

    Ekatherina Stoyanova

    Full Text Available The hereditary β-thalassemia major condition requires regular lifelong blood transfusions. Transfusion-related iron overloading has been associated with the onset of cardiovascular complications, including cardiac dysfunction and vascular anomalies. By using an untransfused murine model of β-thalassemia major, we tested the hypothesis that vascular endothelial dysfunction, alterations of arterial structure and of its mechanical properties would occur despite the absence of treatments.Vascular function and structure were evaluated ex vivo. Compared to the controls, endothelium-dependent vasodilation with acetylcholine was blunted in mesenteric resistance arteries of β-thalassemic mice while the endothelium-independent vasodilator (sodium nitroprusside produced comparable vessel dilation, indicating endothelial cell impairment with preserved smooth muscle cell reactivity to nitric oxide (NO. While these findings suggest a decrease in NO bioavailability, Western blotting showed heightened expression of aortic endothelial NO synthase (eNOS in β-thalassemia. Vascular remodeling of the common carotid arteries revealed increased medial elastin content. Under isobaric conditions, the carotid arteries of β-thalassemic mice exhibited decreased wall stress and softening due to structural changes of the vessel wall.A complex vasculopathy was identified in untransfused β-thalassemic mice characterized by altered carotid artery structure and endothelial dysfunction of resistance arterioles, likely attributable to reduced NO bioavailability despite enhanced vascular eNOS expression.

  12. Differential effect of beetroot bread on postprandial DBP according to Glu298Asp polymorphism in the eNOS gene: a pilot study.

    Science.gov (United States)

    Hobbs, D A; George, T W; Lovegrove, J A

    2014-12-01

    Our objective was to investigate whether the presence of Glu298Asp polymorphism in the endothelial NO synthase (eNOS) gene differentially affects the postprandial blood pressure response to dietary nitrate-rich beetroot bread. A randomised, single-blind, controlled, crossover acute pilot study was performed in 14 healthy men (mean age: 34±9 years) who were retrospectively genotyped for Glu298Asp polymorphism (7GG; T carriers 7). Volunteers were randomised to receive 200 g beetroot-enriched bread (1.1 mmol nitrate) or control bread (no beetroot; 0.01 mmol nitrate) on two separate occasions 10 days apart. Baseline and incremental area under the curve of blood pressure and NOx (nitrate/nitrite) were measured for a 6-h postprandial period. A treatment × genotype interaction was observed for diastolic blood pressure (Pconsumption of beetroot bread compared with control bread. No significant differences were observed in the GG group. The beneficial diastolic blood pressure reduction was observed only in the T carriers of the Glu298Asp polymorphism in the eNOS gene after consumption of nitrate-rich beetroot bread. These data require confirmation in a larger population group.

  13. Hepatopulmonary syndrome induced by common bile duct ligation in a rabbit model: correlation between pulmonary vascular dilatation on thin-section CT and angiography and serum nitrite concentration or endothelial nitric oxide synthase (eNOS)1 expression

    International Nuclear Information System (INIS)

    Lee, Ki Nam; Yoon, Seong Kuk; Lee, Jin Wha; Kim, Ki Nam; Park, Byung Ho; Kwak, Jong Young; Jeong, Jin Sook; Kim, Young Hoon

    2004-01-01

    To investigate the correlation between radiologic vascular dilatation and serum nitrite concentration and eNOS expression in the endothelial cell and pneumocyte in a rabbit model of hepatopulmonary syndrome induced by common bile duct ligation (CBDL). Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n=6), or a sham operation (n=4), and intrapulmonary vasodilatation was assessed. The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining. The diameters of pulmonary vessels on thin-section CT were well correlated with nitrite concentrations in serum (r=0.92, ρ < 0.001). Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r=0.94, ρ < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r=0.90, ρ < 0.001). The peripheral pulmonary vasculature in hepatopulmonary syndrome induced by CBLD was dilated on thin-section CT and on angiographs. Our findings suggest that peripheral pulmonary vascular dilatations are correlated with serum nitrite concentrations and pulmonary eNOS expression

  14. L-Arginine ameliorates cardiac left ventricular oxidative stress by upregulating eNOS and Nrf2 target genes in alloxan-induced hyperglycemic rats

    International Nuclear Information System (INIS)

    Ramprasath, Tharmarajan; Hamenth Kumar, Palani; Syed Mohamed Puhari, Shanavas; Senthil Murugan, Ponniah; Vasudevan, Varadaraj; Selvam, Govindan Sadasivam

    2012-01-01

    Highlights: ► L-Arginine treatment reduced the metabolic disturbances in diabetic animals. ► Antioxidant marker proteins were found high in myocardium by L-arginine treatment. ► Elevated antioxidant status, mediates the reduced TBA-reactivity in left ventricle. ► L-Arginine treatment enhanced the Nrf2 and eNOS signaling in left ventricle. ► Improved cell survival signaling by arginine, offers a novel tactic for targeting. -- Abstract: Hyperglycemia is independently related with excessive morbidity and mortality in cardiovascular disorders. L-Arginine-nitric oxide (NO) pathway and the involvement of NO in modulating nuclear factor-E2-related factor-2 (Nrf2) signaling were well established. In the present study we investigated, whether L-arginine supplementation would improve the myocardial antioxidant defense under hyperglycemia through activation of Nrf2 signaling. Diabetes was induced by alloxan monohydrate (90 mg kg −1 body weight) in rats. Both non-diabetic and diabetic group of rats were divided into three subgroups and they were administered either with L-arginine (2.25%) or L-NAME (0.01%) in drinking water for 12 days. Results showed that L-arginine treatment reduced the metabolic disturbances in diabetic rats. Antioxidant enzymes and glutathione levels were found to be increased in heart left ventricles, thereby reduction of lipid peroxidation by L-arginine treatment. Heart histopathological analysis further validates the reversal of typical diabetic characteristics consisting of alterations in myofibers and myofibrillary degeneration. qRT-PCR studies revealed that L-arginine treatment upregulated the transcription of Akt and downregulated NF-κB. Notably, transcription of eNOS and Nrf2 target genes was also upregulated, which were accompanied by enhanced expression of Nrf2 in left ventricular tissue from diabetic and control rats. Under these findings, we suggest that targeting of eNOS and Nrf2 signaling by L-arginine supplementation could be

  15. L-Arginine ameliorates cardiac left ventricular oxidative stress by upregulating eNOS and Nrf2 target genes in alloxan-induced hyperglycemic rats

    Energy Technology Data Exchange (ETDEWEB)

    Ramprasath, Tharmarajan; Hamenth Kumar, Palani; Syed Mohamed Puhari, Shanavas; Senthil Murugan, Ponniah; Vasudevan, Varadaraj [Molecular Cardiology Unit, Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, Tamilnadu (India); Selvam, Govindan Sadasivam, E-mail: drselvamgsbiochem@rediffmail.com [Molecular Cardiology Unit, Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, Tamilnadu (India)

    2012-11-23

    Highlights: Black-Right-Pointing-Pointer L-Arginine treatment reduced the metabolic disturbances in diabetic animals. Black-Right-Pointing-Pointer Antioxidant marker proteins were found high in myocardium by L-arginine treatment. Black-Right-Pointing-Pointer Elevated antioxidant status, mediates the reduced TBA-reactivity in left ventricle. Black-Right-Pointing-Pointer L-Arginine treatment enhanced the Nrf2 and eNOS signaling in left ventricle. Black-Right-Pointing-Pointer Improved cell survival signaling by arginine, offers a novel tactic for targeting. -- Abstract: Hyperglycemia is independently related with excessive morbidity and mortality in cardiovascular disorders. L-Arginine-nitric oxide (NO) pathway and the involvement of NO in modulating nuclear factor-E2-related factor-2 (Nrf2) signaling were well established. In the present study we investigated, whether L-arginine supplementation would improve the myocardial antioxidant defense under hyperglycemia through activation of Nrf2 signaling. Diabetes was induced by alloxan monohydrate (90 mg kg{sup -1} body weight) in rats. Both non-diabetic and diabetic group of rats were divided into three subgroups and they were administered either with L-arginine (2.25%) or L-NAME (0.01%) in drinking water for 12 days. Results showed that L-arginine treatment reduced the metabolic disturbances in diabetic rats. Antioxidant enzymes and glutathione levels were found to be increased in heart left ventricles, thereby reduction of lipid peroxidation by L-arginine treatment. Heart histopathological analysis further validates the reversal of typical diabetic characteristics consisting of alterations in myofibers and myofibrillary degeneration. qRT-PCR studies revealed that L-arginine treatment upregulated the transcription of Akt and downregulated NF-{kappa}B. Notably, transcription of eNOS and Nrf2 target genes was also upregulated, which were accompanied by enhanced expression of Nrf2 in left ventricular tissue from diabetic

  16. eNOS se correlaciona com a biogênese mitocondrial em corações com cardiopatia congênita e cianose

    Directory of Open Access Journals (Sweden)

    Juan Xiao

    2012-09-01

    Full Text Available FUNDAMENTO: O programa de biogênese mitocondrial no coração parece apresentar remodelação adaptativa após estresse biomecânico e oxidativo. Os mecanismos adaptativos que protegem o metabolismo do miocárdio durante a hipóxia são coordenados, em parte, pelo óxido nítrico (NO. OBJETIVO: Observar a biogênese mitocondrial e expressão do óxido nítrico sintase (NOS em corações de cardiopatia congênita com cianose; discutir a resposta mitocondrial à hipóxia crônica do miocárdio. MÉTODOS: Foram investigados 20 pacientes com defeitos cardíacos cianóticos (n = 10 ou acianóticos (n = 10. Foram estudadas amostras do miocárdio na via de saída ventricular direita, tomadas durante a operação. A análise morfométrica de mitocôndrias foi realizada por microscopia eletrônica de transmissão. A relação mtDNA/nDNA foi determinada com PCR em tempo real. Os níveis de transcrição da subunidade I da citocromo c oxidase (COXI, coativador-1α do receptor γ ativado por proliferador de peroxissoma (PGC-1α, o fator respiratório nuclear 1 (NRF1, e fator de transcrição mitocondrial A (Tfam foram detectados por reação em cadeia da polimerase via transcriptase reversa (RT-PCR ativado por fluorescência em tempo real. Os níveis proteicos de COXI e nNOS, iNOS e eNOS foram medidos por técnica de Western Blot. RESULTADOS: A densidade volumétrica mitocondrial (Vv e a densidade numérica (Nv foram significativamente elevadas em pacientes com cianose, em comparação com a cardiopatia congênita acianótica. MtDNA elevada e suprarregulação dos níveis de COXI, PGC-1 α, NRF1 e Tfam mRNA foram observadas em pacientes cianóticos. Os níveis de proteína de COXI e eNOS foram significativamente maiores no miocárdio de pacientes cianóticos que nos de acianóticos. Os níveis de transcrição do PGC-1α se correlacionam com os níveis de eNOS. CONCLUSÃO: A biogênese mitocondrial é ativada no miocárdio da via de saída ventricular na

  17. Age and gender differences in the influences of eNOS T-786C polymorphism on arteriosclerotic parameters in general population in Japan.

    Science.gov (United States)

    Hashimoto, Marowa; Miyai, Nobuyuki; Hattori, Sonomi; Iwahara, Akihiko; Utsumi, Miyoko; Arita, Mikio; Takeshita, Tatsuya

    2016-07-01

    The influence of T-786C polymorphism in the promoter region of endothelial nitric oxide synthase (eNOS) on arteriosclerotic parameters by age and gender were examined. Brachial-ankle pulse wave velocity (baPWV), heart-rate adjusted augmentation index (AIx@75), pulse pressure (PP) and albumin-creatinine ratio (ACR) were assessed as arteriosclerotic parameters in addition to non-high-density lipoprotein cholesterol (non-HDL-C) to HDL-C (non-HDL-C/HDL-C) ratio in 1499 participants. T-786C polymorphism (rs2070744) was screened using a TaqMan allelic discrimination assay. Analyses of covariance were carried. Women with the non-C allele showed significantly lower AIx@75 in participants aged gender differences, possibly involving antioxidative and/or endothelial signaling other than inflammatory signaling.

  18. H2O2 Treatment of HUVECs Facilitates PKC Mediated Thr495 Phosphorylation on eNOS when Pre-treated with High Glucose Levels

    DEFF Research Database (Denmark)

    Guterbaum, Thomas J; Braunstein, Thomas H; Fossum, Anna

    2015-01-01

    Objective: Metabolic syndrome entails hypertension, hyperglycemia, obesity and hypercholesterolemia. This syndrome increases the risk of cardiovascular disease and diabetes. Hyperglycemia during coronary reperfusion is associated with a poor prognosis. Contrastingly, targeting correction of hyper......Objective: Metabolic syndrome entails hypertension, hyperglycemia, obesity and hypercholesterolemia. This syndrome increases the risk of cardiovascular disease and diabetes. Hyperglycemia during coronary reperfusion is associated with a poor prognosis. Contrastingly, targeting correction....... The presence of reactive oxygen species (ROS) in both mitochondria and cytoplasm was measured by fluorescence activated cell sorting (FACS). Phosphorylation of endothelial nitric oxide synthase (eNOS) on threonine 495 (Thr495) and serine 1177 (Ser1177) was assessed by western blotting. Short-term (20 hours...

  19. 2,4,6-Tri-amino-1,3,5-triazin-1-ium 3-(prop-2-eno-yloxy)propano-ate acrylic acid monosolvate monohydrate.

    Science.gov (United States)

    Sangeetha, V; Kanagathara, N; Chakkaravarthi, G; Marchewka, M K; Anbalagan, G

    2013-05-01

    The asymmetric unit of the title salt, C3H7N6 (+)·C6H7O4 (-)·C3H4O2·H2O, contains a 2,4,6-tri-amino-1,3,5-triazin-1-ium cation, a 3-(prop-2-eno-yloxy)propano-ate anion and acrylic acid and water solvent mol-ecules in a 1:1:1:1 ratio and with each species in a general position. In the crystal, the components are linked into a supra-molecular layer in the bc plane via a combination of O-H⋯O, N-H⋯N and N-H⋯O hydrogen bonding. The crystal studied was a non-merohedral twin, the minor component contribution being approximately 26%.

  20. Treadmill exercise alleviates diabetic cardiomyopathy by suppressing plasminogen activator inhibitor expression and enhancing eNOS in streptozotocin-induced male diabetic rats.

    Science.gov (United States)

    Chengji, Wang; Xianjin, Fan

    2018-04-01

    To investigate the biological mechanism of the effect of different intensity exercises on diabetic cardiomyopathy. 87 raise specific pathogen SPF healthy 6-week-old male Sprague-Dawley rats, fed 6 weeks with high-fat diet for rats were used, and a diabetic model was established by intraperitoneal injection of streptozotocin - randomly selected 43 rats were divided into Diabetic control group (DCG, n  = 10), Diabetic exercise group 1 (DEG1, n  = 11), Diabetic exercise group 2 (DEG2, n  = 11) and Diabetic exercise group 3 (DEG3, n  = 11). The rats in DEG1 were forced to run on a motorized treadmill, the exercise load consisted of running at a speed of 10 m/min, the exercise load of the rats in DEG2 were running at a speed of 15 m/min, the exercise load of the rats in DEG3 were running at a speed of 20 m/min, for one hour once a day for 6 weeks. After 6 weeks of exercise intervention, glucose metabolism-related indexes in rats such as blood glucose (FBG), glycosylated serum protein (GSP) and insulin (FINS); cardiac fibrinolytic system parameters such as PAI-1 (plasminogen activator inhibitor 1), Von Willebrand factor (vWF), protein kinase C (PKC) and diacylglycerol (DAG); and serum level of NO, eNOS and T-NOS were measured. Compared with DCG, fasting blood glucose and GSP were decreased, while insulin sensitivity index and insulin level were increased in all rats of the three exercise groups. FBG decrease was statistically significant ( P  diabetic rats; myocardial PAI-1 in DEG1, DEG2 and DEG3 rats decreased significantly ( P  diabetic cardiomyopathy by affecting the levels of PAI-1 and eNOS, and there is a dependence on intensity. © 2018 The authors.

  1. San-Huang-Xie-Xin-Tang Prevents Rat Hearts from Ischemia/Reperfusion-Induced Apoptosis through eNOS and MAPK Pathways

    Directory of Open Access Journals (Sweden)

    Shu-Fen Liou

    2011-01-01

    Full Text Available San-Huang-Xie-Xin-Tang (SHXT is a traditional Chinese medication consisting of three herbs, namely Coptidis rhizome, Scutellariae radix and Rhei rhizome. This study aimed to examine the cardioprotective effects of SHXT in a rat model of acute myocardial apoptosis induced by ischemia/reperfusion (I/R. Vehicle (intravenous saline or SHXT (intravenous or oral was administered prior to I/R (occlusion of left coronary artery for 45 min followed by reperfusion for 2 h. In the vehicle group, myocardial I/R caused myocardial infarction with increased plasma cardiac enzymes, severe arrhythmia and mortality. Myocardial apoptosis was induced by I/R as evidenced by DNA ladder and Bcl-2/Bax ratio. In the SHXT group, we found that SHXT significantly reduced plasma levels of cardiac enzymes, arrhythmia scores (from 5 ± 1 to 2 ± 1, P<.01 and mortality rate (from 53 to 0%, P<.01. In addition, pretreatment with intravenous SHXT reduced the infarct size dose-dependently when compared with the vehicle group (10 mg kg−1: 14.0 ± 0.2 versus 44.5 ± 5.0%, and 30 mg kg−1: 6.2 ± 1.2% versus 44.5 ± 5.0%, both P<.01. Similarly, oral administration of SHXT reduced the infarct size dose-dependently. Furthermore, SHXT markedly decreased the apoptosis induced by I/R with increased Bcl-2/Bax ratio. Finally, we found that SHXT counteracted the I/R-induced downstream signaling, resulting in increased myocardial eNOS expression and plasma nitrite, and decreased activation of ERK1/2, p38 and JNK. These data suggest that SHXT has cardioprotective effects against I/R-induced apoptosis, and that these effects are mediated, at least in part, by eNOS and MAPK pathways.

  2. Role of C677T and A1298C MTHFR, A2756G MTR and -786 C/T eNOS gene polymorphisms in atrial fibrillation susceptibility.

    Directory of Open Access Journals (Sweden)

    Betti Giusti

    Full Text Available BACKGROUND: Hyperhomocysteinemia has been suggested to play a role in the NonValvular Atrial Fibrillation (NVAF pathogenesis. Polymorphisms in genes coding for homocysteine (Hcy metabolism enzymes may be associated with hyperhomocysteinemia and NVAF. METHODOLOGIES: 456 NVAF patients and 912 matched controls were genotyped by an electronic microchip technology for C677T and A1298C MTHFR, A2756G MTR, and -786C/T eNOS gene polymorphisms. Hcy was determined by an immunoassay method. PRINCIPAL FINDINGS: The genotype distribution of the four polymorphisms as well as genotype combinations did not differ in patients and controls. Hcy was higher in patients than in controls (15.2, 95%CI 14.7-15.7 vs 11.3, 95%CI 11.0-11.6 micromol/L; p<0.0001. In both populations, a genotype-phenotype association (p<0.0001 between Hcy and C677T MTHFR polymorphism was observed; in controls a significant (p = 0.029 association between tHcy and -786C/T eNOS polymorphism was also observed. At the multivariate analysis the NVAF risk significantly increased in the upper quartiles of Hcy compared to the lowest: OR from 2.8 (1.68-4.54 95%CI in Q2 to 12.9 (7.96-21.06 95%CI in Q4. CONCLUSIONS: Our data demonstrated the four polymorphisms, although able, at least in part, to affect Hcy, were not associated with an increased risk of NVAF per se or in combination.

  3. Dual-Specificity Phosphatase 4 Overexpression in Cells Prevents Hypoxia/Reoxygenation-Induced Apoptosis via the Upregulation of eNOS

    Science.gov (United States)

    Dougherty, Julie A.; Kilbane Myers, Joanna; Khan, Mahmood; Angelos, Mark G.; Chen, Chun-An

    2017-01-01

    Mitogen-activated protein kinases (MAPKs) signaling cascades regulate several cellular functions, including differentiation, proliferation, survival, and apoptosis. The duration and magnitude of phosphorylation of these MAPKs are decisive determinants of their physiological functions. Dual-specificity phosphatases exert kinetic control over these signaling cascades. Previously, we demonstrated that DUSP4−/− hearts sustain a larger infarct and have poor functional recovery, when isolated hearts were subjected to ischemia/reperfusion. Uncontrolled p38 activation and upregulation of Nox4 expression are the main effectors for this functional alteration. Here, dual-specificity phosphatase 4 (DUSP4) overexpression in endothelial cells was used to investigate the role of DUSP4 on the modulation of reactive oxygen species (ROS) generation and vascular function, when cells were subjected to hypoxia/reoxygenation (H/R) insult. Immunostaining with cleaved caspase-3 revealed that DUSP4 overexpression prevents caspase-3 activation and apoptosis after H/R. The beneficial effects occur via modulating p38 activity, increased NO bioavailability, and reduced oxidative stress. More importantly, DUSP4 overexpression upregulates eNOS protein expression (1.62 ± 0.33 versus 0.65 ± 0.16) during H/R-induced stress. NO is a critical small molecule involved in regulating vascular tone, vascular growth, platelet aggregation, and modulation of inflammation. The level of NO generation determined using DAF-2 fluorescence demonstrated that DUSP4 overexpression augments NO production and thus improves vascular function. The level of superoxide generated from cells after being subjected to H/R was determined using dihydroethidium-HPLC method. The results suggested that DUSP4 overexpression in cells decreases H/R-induced superoxide generation (1.56 ± 0.14 versus 1.19 ± 0.05) and thus reduces oxidant stress. This also correlates with the reduction in the total protein S

  4. Obesity, inflammation, and exercise training: relative contribution of iNOS and eNOS in the modulation of vascular function in the mouse aorta

    Directory of Open Access Journals (Sweden)

    Josiane Fernandes da Silva

    2016-09-01

    Full Text Available Background - The understanding of obsesity-related vascular dysfunction remains controversial mainly because of the diseases associated with vascular injury. Exercise training is known to prevent vascular dysfunction. Using an obesity model without comorbidities, we aimed at investigating the underlying mechanism of vascular dysfunction and how exercise interferes with this process.Methods - High-sugar diet was used to induce obesity in mice. Exercise training was performed 5 days/week. Body weight, energy intake, and adipose tissues were assessed; blood metabolic and hormonal parameters were determined; and serum TNFα was measured. Blood pressure and heart rate were assessed by plethysmography. Changes in aortic isometric tension were recorded on myograph. Western blot was used to analyze protein expression. Nitric oxide (NO was evaluated using fluorescence microscopy. Antisense oligodeoxynucleotides were used for inducible nitric oxide synthase isoform (iNOS knockdown.Results - Body weight, fat mass, total cholesterol, low-density lipoprotein cholesterol fraction, insulin, and leptin were higher in the sedentary obese group (SD than in the sedentary control animals (SS. Exercise training prevented these changes. No difference in glucose tolerance, insulin sensitivity, blood pressure, and heart rate was found. Decreased vascular relaxation and reduced endothelial nitric oxide synthase (eNOS functioning in the SD group were prevented by exercise. Contractile response to phenylephrine was decreased in the aortas of the wild SD mice, compared with that of the SS group; however, no alteration was noted in the SD iNOS-/- animals. The decreased contractility was endothelium-dependent, and was reverted by iNOS inhibition or iNOS silencing. The aortas from the SD group showed increased basal NO production, serum TNFα, TNF receptor-1, and phospho-IκB. Exercise training attenuated iNOS-dependent reduction in contractile response in high-sugar diet

  5. Effects of stimulation of soluble guanylate cyclase on diabetic nephropathy in diabetic eNOS knockout mice on top of angiotensin II receptor blockade.

    Directory of Open Access Journals (Sweden)

    Ina M Ott

    Full Text Available The prevalence of diabetes mellitus and its complications, such as diabetic nephropathy (DN, is rising worldwide and prevention and treatment are therefore becoming increasingly important. Therapy of DN is particularly important for patients who do not adequately respond to angiotensin receptor blocker (ARB treatment. Novel approaches include the stimulation of soluble guanylate cyclase (sGC as it is reported to have beneficial effects on cardiac and renal damage. We aimed to investigate the effects of the sGC stimulator riociguat and ARB telmisartan on kidney function and structure in a hypertensive model of diabetic nephropathy. Seventy-six diabetic male eNOS knockout C57BL/6J mice were randomly divided after having received streptozotocin: telmisartan (1 mg/kg/d, riociguat (3 mg/kg/d, riociguat+telmisartan (3+1 mg/kg/d, and vehicle. Fourteen mice were used as non-diabetic controls. Treatment duration was 11 weeks. Glucose concentrations were increased and similar in all diabetic groups. Telmisartan insignificantly reduced blood pressure by 5.9 mmHg compared with diabetic controls (111.2±2.3 mmHg vs. 117.1±2.2 mmHg; p = 0.071. Treatment with riociguat both alone and in combination with telmisartan led to a significant reduction of blood pressure towards diabetic vehicle (105.2±2.5 mmHg and 105.0±3.2 mmHg, respectively, vs. 117.1±2.2 mmHg. Combined treatment also significantly decreased albuminuria compared with diabetic controls (47.3±9.6 µg/24 h vs. 170.8±34.2 µg/24 h; p = 0.002 reaching levels similar to those of non-diabetic controls (34.4±10.6 µg/24 h, whereas the reduction by single treatment with either telmisartan (97.8±26.4 µg/24 h or riociguat (97.1±15.7 µg/24 h was not statistically significant. The combination treatment led to a significant (p<0.01 decrease of tissue immunoreactivity of malondialdehyde, as consequence of reduced oxidative stress. In conclusion, stimulation of sGC significantly reduced urinary

  6. Teine nahk ehk Ornament ja meie / Hanno Soans

    Index Scriptorium Estoniae

    Soans, Hanno, 1974-

    2003-01-01

    Arhitekt Adolf Loosi suhtumine ornamenti ja nahakaunistustesse, tattoo tagasitulek käibesse läänes XX sajandi II poolel. EKA nahakunsti eriala diplomandi Kerstin Raidma lõputööst, mis sisaldas seeria levinumaid tätoveeringupiirkondi katvaid nahast ehteid, lühidalt sama eriala lõpetaja Agnetha Kurusoni lõputööst "Kest" ja lõputööde hinnetest

  7. Algas "Digipöörde" teine hooaeg! / Raivo Juurak

    Index Scriptorium Estoniae

    Juurak, Raivo, 1949-

    2015-01-01

    Ülemiste tehnoloogialinnakus toimus Samsung Electronics Balticsi ja Tallinna Ülikooli algatatud koolitusprogrammi "Digipööre" teise aasta avaüritus. Selgitusi jagas projekti koolitusjuht Mart Laanpere

  8. Uut moodi Teine maailmasõda / Kaisa Karu

    Index Scriptorium Estoniae

    Karu, Kaisa

    2009-01-01

    Teise maailmasõja 70. aastapäevaks valminud kuueosalise doksarja "Apokalüpsis: WWII", (National Geographic, režissöör Isabelle Clarke, stsenaristid Henri de Turenne ja Daniel Costelle) filmimispaikades

  9. Gaasisõja teine vaatus Valgevenes / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2006-01-01

    Valgevene ja Gazprom pole jõudnud kokkuleppele Valgevenele tarnitava maagaasi hinna tõusus. Gazprom ähvardab järsult vähendada Valgevenesse tarnitava maagaasi kogust, Valgevene ähvardab tõsta maagaasi transiiditasu. Lisa: Kaukaasia valmistub samuti külmaks talveks

  10. Tera siit, teine sealt - keskpõrandale kokku! / Rein Tootmaa

    Index Scriptorium Estoniae

    Tootmaa, Rein

    2003-01-01

    7. Pimedate Ööde Filmifestivali filme - sakslase Hans-Christian Schmid'i mängufilm "Kauged tuled" ("Lichter"), taanlase Lars von Trieri "Dogville", norralase Morte Tydlumi "Semu" ("Buddy"), tema kaasmaalase Jens Lieni ("Jonny Vang"), jaapanlase Takeshi Kitano "Nukud" ("Dolls") ja taanlase Jesper W. Nielseni "Mees ukse taga" ("Manden bag doren")

  11. Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47phox Expression in Rats

    Directory of Open Access Journals (Sweden)

    Thewarid Berkban

    2015-06-01

    Full Text Available The effect of ellagic acid on oxidative stress and hypertension induced by Nω-Nitro-l-arginine methyl ester hydrochloride (L-NAME was investigated. Male Sprague-Dawley rats were administrated with L-NAME (40 mg/kg/day for five weeks. L-NAME induced high systolic blood pressure (SBP and increased heart rate (HR, hindlimb vascular resistance (HVR and oxidative stress. Concurrent treatment with ellagic acid (7.5 or 15 mg/kg prevented these alterations. Co-treatment with ellagic acid was associated with up-regulation of endothelial nitric oxide synthase (eNOS protein production and alleviation of oxidative stress as indicated by decreased superoxide production in the vascular tissue, reduced plasma malondialdehyde levels, reduced NADPH oxidase subunit p47phox expression and increased plasma nitrate/nitrite levels. Our results indicate that ellagic acid attenuates hypertension by reducing NADPH oxidase subunit p47phox expression, which prevents oxidative stress and restores NO bioavailability.

  12. Synergistic Antihypertensive Effect of Carthamus tinctorius L. Extract and Captopril in l-NAME-Induced Hypertensive Rats via Restoration of eNOS and AT1R Expression

    Directory of Open Access Journals (Sweden)

    Putcharawipa Maneesai

    2016-02-01

    Full Text Available This study examined the effect of Carthamus tinctorius (CT extract plus captopril treatment on blood pressure, vascular function, nitric oxide (NO bioavailability, oxidative stress and renin-angiotensin system (RAS in Nω-Nitro-l-arginine methyl ester (l-NAME-induced hypertension. Rats were treated with l-NAME (40 mg/kg/day for five weeks and given CT extract (75 or 150 or 300 or 500 mg/kg/day: captopril (5 mg/kg/day or CT extract (300 mg/kg/day plus captopril (5 mg/kg/day for two consecutive weeks. CT extract reduced blood pressure dose-dependently, and the most effective dose was 300 mg/kg/day. l-NAME-induced hypertensive rats showed abnormalities including high blood pressure, high vascular resistance, impairment of acetylcholine-induced vasorelaxation in isolated aortic rings and mesenteric vascular beds, increased vascular superoxide production and plasma malondialdehyde levels, downregulation of eNOS, low level of plasma nitric oxide metabolites, upregulation of angiotensin II type 1 receptor and increased plasma angiotensin II. These abnormalities were alleviated by treatment with either CT extract or captopril. Combination treatment of CT extract and captopril normalized all the abnormalities found in hypertensive rats except endothelial dysfunction. These data indicate that there are synergistic antihypertensive effects of CT extract and captopril. These effects are likely mediated by their anti-oxidative properties and their inhibition of RAS.

  13. Influence of training status and eNOS haplotypes on plasma nitrite concentrations in normotensive older adults: a hypothesis-generating study.

    Science.gov (United States)

    da Silva, Roberta Fernanda; Sertório, Jonas Tadeu Cau; Lacchini, Riccardo; Trapé, Atila Alexandre; Tanus-Santos, José Eduardo; Rush, James W E; Amaral, Sandra Lia; Zago, Anderson Saranz

    2014-12-01

    The purpose of this study was to evaluate the relationship between 3 eNOS gene polymorphisms and training status (TS) in affecting plasma nitrite concentration (NO2) in normotensive adults over 50 years old. Resting blood pressure (BP) was measured in all participants (n = 101). Plasma was taken to analyze: lipid profile, nitrite concentration (NO2) and lipid peroxide levels (T-BARS). Also, genomic DNA was extracted from plasma for genotyping NOS3 polymorphisms (-786T>C; 894G>T; and VNTR in intron 4). TS was determined by one-mile walk test and Functional Fitness Test Battery from AAHPERD (TS1-regular TS; TS2-good TS; and TS3-very good TS). BP was not influenced by TS, but NO2 was 15% higher in TS3 (123 ± 27 nM) compared to TS-2 (106 ± 22 nM). No differences were found in plasma NO2 in the haplotype analyses. However, the presence of the C allele (T-786C) and ASP allele (Glu298Asp) was found to enhance the correlation between TS and NO2 levels (r = 0.492 in C/4b/ASP haplotype and r = 0.855 in C/4a/ASP haplotype). This study thus identifies NOS3 polymorphism-dependent sensitivity to the effects of physical training on plasma NO2. Maintenance of good levels of training status, in carriers of C allele for T-786C polymorphism, combined with ASP allele for Glu298Asp polymorphism, may result in an increase in the NO2 plasma concentrations, which may reflect improved NO bioavailability in older adult normotensive individuals.

  14. A combination of genistein and magnesium enhances the vasodilatory effect via an eNOS pathway and BK(Ca) current amplification.

    Science.gov (United States)

    Sun, Lina; Hou, Yunlong; Zhao, Tingting; Zhou, Shanshan; Wang, Xiaoran; Zhang, Liming; Yu, Guichun

    2015-04-01

    The phytoestrogen genistein (GST) and magnesium have been independently shown to regulate vascular tone; however, their individual vasodilatory effects are limited. The aim of this study was to examine the combined effects of GST plus magnesium on vascular tone in mesenteric arteries. The effects of pretreatment with GST (0-200 μmol/L), MgCl2 (0-4.8 mmol/L) and GST plus MgCl2 on 10 μmol/L phenylephrine (PE) precontracted mesenteric arteries in rats were assessed by measuring isometric force. BK(Ca) currents were detected by the patch clamp method. GST caused concentration- and partial endothelium-dependent relaxation. Magnesium resulted in dual adjustment of vascular tone. Magnesium-free solution eliminated the vasodilatation of GST in both endothelium-intact and denuded rings. GST (50 μmol/L) plus magnesium (4.8 mmol/L) caused stronger relaxation in both endothelium-intact and denuded rings. Pretreatment with the nitric oxide synthase (NOS) inhibitor L-N-nitroarginine methyl ester (L-NAME, 100 μmol/L) significantly inhibited the effects of GST, high magnesium, and the combination of GST and magnesium. BK(Ca) currents were amplified to a greater extent when GST (50 μmol/L) was combined with 4.8 versus 1.2 mmol/L Mg(2+). Our data suggest that GST plus magnesium provides enhanced vasodilatory effects in rat mesenteric arteries compared with that observed when either is used separately, which was related to an eNOS pathway and BK(Ca) current amplification.

  15. MicroRNA-27b plays a role in pulmonary arterial hypertension by modulating peroxisome proliferator-activated receptor γ dependent Hsp90-eNOS signaling and nitric oxide production

    Energy Technology Data Exchange (ETDEWEB)

    Bi, Rui; Bao, Chunrong; Jiang, Lianyong; Liu, Hao; Yang, Yang; Mei, Ju; Ding, Fangbao, E-mail: dbcar126@126.com

    2015-05-01

    Pulmonary artery endothelial dysfunction is associated with pulmonary arterial hypertension (PAH). Based on recent studies showing that microRNA (miR)-27b is aberrantly expressed in PAH, we hypothesized that miR-27b may contribute to pulmonary endothelial dysfunction and vascular remodeling in PAH. The effect of miR-27b on pulmonary endothelial dysfunction and the underlying mechanism were investigated in human pulmonary artery endothelial cells (HPAECs) in vitro and in a monocrotaline (MCT)-induced model of PAH in vivo. miR-27b expression was upregulated in MCT-induced PAH and inversely correlated with the levels of peroxisome proliferator-activated receptor (PPAR)-γ, and miR-27b inhibition attenuated MCT-induced endothelial dysfunction and remodeling and prevented PAH associated right ventricular hypertrophy and systolic pressure in rats. PPARγ was confirmed as a direct target of miR-27b in HPAECs and shown to mediate the effect of miR-27b on the disruption of endothelial nitric oxide synthase (eNOS) coupling to Hsp90 and the suppression of NO production associated with the PAH phenotype. We showed that miR-27b plays a role endothelial function and NO release and elucidated a potential mechanism by which miR-27b regulates Hsp90-eNOS and NO signaling by modulating PPARγ expression, providing potential therapeutic targets for the treatment of PAH. - Highlights: • miR-27b plays a role in endothelial function and NO release. • miR-27b inhibition ameliorates MCT-induced endothelial dysfunction and PAH. • miR-27b targets PPARγ in HPAECs. • miR-27b regulates PPARγ dependent Hsp90-eNOS and NO signaling.

  16. Impacto del ENOS sobre los rendimientos de maíz en la región pampeana argentina Impact of the ENSO on corn yields in the Argentinean pampas region

    Directory of Open Access Journals (Sweden)

    M. E. Fernández Long

    2011-06-01

    Full Text Available El fenómeno de “El Niño - Oscilación del Sur” (ENOS es uno de los principales responsables de la variabilidad climática interanual, con influencia sobre la región pampeana argentina. El objetivo de este trabajo fue analizar el impacto del ENOS sobre las variaciones anuales de la producción de maíz. La región fue dividida en 10 zonas en las cuales los rendimientos tienen una variabilidad interanual homogénea. Se utilizaron diferentes índices del ENOS. Los resultados indicaron que, en la mayor parte de la región, los desvíos de los rendimientos son, en general, positivos durante eventos “El Niño” y negativos durante “La Niña”. Sin embargo, el impacto es muy fuerte al norte y centro de la región analizada, y se debilita hacia el sur. En el sudeste de la provincia de Buenos Aires el comportamiento es diferente que en el resto. Los índices promediados de mayo a julio son, en general, un buen indicador de la influencia del ENOS sobre la productividad de los cultivos, y brindan una adecuada información para la toma de decisiones de la empresa agropecuaria, con suficiente anticipación respecto del comienzo de la campaña.The “El Niño Southern Oscillation” (ENSO phenomenon is one of the main responsible factor for interannual climate variability in the “pampas” region. This work aims to analyze the impact of ENSO on the annual variations of corn production. The region was divided into 10 areas in which yields have a homogeneous interannual variability. Five ENSO indexes were used The results indicated that, in most parts of the region, the deviations of trend line yields are, in general, positive during “El Niño” events and negative during “La Niña”. However, the impact is very strong in the North and Centre of the analyzed region and weakens southward. In the southeast of the province of Buenos Aires, the behavior is different than the rest. Averaging indexes from May to July we obtained a good indicator

  17. Puruvana puruvanavana : [luuletused] / Eno Raud

    Index Scriptorium Estoniae

    Raud, Eno, 1928-1996

    2000-01-01

    Sisu: Puruvana puruvanavana ; Vetesell ; Pada ja katel ; Nääripäkapikk ; Vanapaar ; Mere peremees ; Lepamaim ; Kiilaspää kiilasjääl ; Tõugu unistus ; Naljatilk silk ; Muttide jalutuskäik ; Hiir lumes ; Kalakari salakaril ; Naljalammas ; Mesilastest ; Kure mure ; Voki laul karule ; Põrsalaul ; Kurk ja kõrvits ; Tuhk ja tina ; Õige pea ja vale pea ; Suu pea, puupea! ; Siga õuel

  18. Efeitos da atorvastatina e do polimorfismo T-786C do gene eNOS sobre parâmetros do metabolismo lipídico plasmático

    Directory of Open Access Journals (Sweden)

    Vanessa Helena de Souza Zago

    2013-01-01

    Full Text Available FUNDAMENTO: A atividade do óxido nítrico sintase endotelial (eNOS pode ser modulada pelo colesterol da lipoproteína de alta densidade (HDL-C, estatinas ou polimorfismos, como o T-786C de eNOS. OBJETIVO: Este estudo teve como objetivo avaliar se o polimorfismo T-786C está associado a alterações nos efeitos da atorvastatina no perfil lipídico, nas concentrações de metabólitos de óxido nítrico (NO e da proteína C reativa de alta sensibilidade (PCR-as. MÉTODOS: Trinta voluntários do sexo masculino, assintomáticos, com idade entre 18-56 anos foram genotipados e classificados de acordo com a ausência (TT, n = 15 ou presença (CC, n = 15 do polimorfismo. Eles foram selecionados aleatoriamente para a utilização de placebo e atorvastatina (10 mg/dia por 14 dias. Após cada tratamento foram medidos lípides, lipoproteínas, frações HDL2 e HDL3, atividade da proteína de transferência de colesteril éster (CETP, metabólitos de NO e PCR-as. RESULTADOS: As comparações entre genótipos após a administração de placebo mostraram aumento da atividade da CETP polimorfismo-dependente (TT, 12 ± 7; CC, 22 ± 12, p < 0,05. As análises da interação entre os tratamentos indicaram que a atorvastatina tem efeito sobre colesterol, LDL, nitrito e razões lípides/proteínas (HDL2 e HDL3 (p < 0,001 em ambos os genótipos. É interessante notar as interações genótipo/droga sobre a CETP (p < 0,07 e a lipoproteína (a [Lp(a] (p < 0,056, levando a uma diminuição limítrofe da CETP, embora sem afetar a Lp(a. A PRC-as não mostrou alterações. CONCLUSÃO: Os resultados sugerem que o tratamento com estatinas pode ser relevante para a prevenção primária da aterosclerose em pacientes com o polimorfismo T-786C do eNOS, considerando os efeitos no metabolismo lipídico.

  19. 2,4,6-Tri­amino-1,3,5-triazin-1-ium 3-(prop-2-eno­yloxy)propano­ate acrylic acid monosolvate monohydrate

    Science.gov (United States)

    Sangeetha, V.; Kanagathara, N.; Chakkaravarthi, G.; Marchewka, M. K.; Anbalagan, G.

    2013-01-01

    The asymmetric unit of the title salt, C3H7N6 +·C6H7O4 −·C3H4O2·H2O, contains a 2,4,6-tri­amino-1,3,5-triazin-1-ium cation, a 3-(prop-2-eno­yloxy)propano­ate anion and acrylic acid and water solvent mol­ecules in a 1:1:1:1 ratio and with each species in a general position. In the crystal, the components are linked into a supra­molecular layer in the bc plane via a combination of O—H⋯O, N—H⋯N and N—H⋯O hydrogen bonding. The crystal studied was a non-merohedral twin, the minor component contribution being approximately 26%. PMID:23723892

  20. The Streptococcus pyogenes Serotype M49 Nra-Ralp3 Transcriptional Regulatory Network and Its Control of Virulence Factor Expression from the Novel eno ralp3 epf sagA Pathogenicity Region▿ †

    Science.gov (United States)

    Kreikemeyer, Bernd; Nakata, Masanobu; Köller, Thomas; Hildisch, Hendrikje; Kourakos, Vassilios; Standar, Kerstin; Kawabata, Shigetada; Glocker, Michael O.; Podbielski, Andreas

    2007-01-01

    Many Streptococcus pyogenes (group A streptococcus [GAS]) virulence factor- and transcriptional regulator-encoding genes cluster together in discrete genomic regions. Nra is a central regulator of the FCT region. Previous studies exclusively described Nra as a transcriptional repressor of adhesin and toxin genes. Here transcriptome and proteome analysis of a serotype M49 GAS strain and an isogenic Nra mutant of this strain revealed the complete Nra regulon profile. Nra is active in all growth phases tested, with the largest regulon in the transition phase. Almost exclusively, virulence factor-encoding genes are repressed by Nra; these genes include the GAS pilus operon, the capsule synthesis operon, the cytolysin-mediated translocation system genes, all Mga region core virulence genes, and genes encoding other regulators, like the Ihk/Irr system, Rgg, and two additional RofA-like protein family regulators. Surprisingly, our experiments revealed that Nra additionally acts as a positive regulator, mostly for genes encoding proteins and enzymes with metabolic functions. Epidemiological investigations revealed strong genetic linkage of one particular Nra-repressed regulator, Ralp3 (SPy0735), with a gene encoding Epf (extracellular protein factor from Streptococcus suis). In a serotype-specific fashion, this ralp3 epf gene block is integrated, most likely via transposition, into the eno sagA virulence gene block, which is present in all GAS serotypes. In GAS serotypes M1, M4, M12, M28, and M49 this novel discrete genetic region is therefore designated the eno ralp3 epf sagA (ERES) pathogenicity region. Functional experiments showed that Epf is a novel GAS plasminogen-binding protein and revealed that Ralp3 activity counteracts Nra and MsmR regulatory activity. In addition to the Mga and FCT regions, the ERES region is the third discrete chromosomal pathogenicity region. All of these regions are transcriptionally linked, adding another level of complexity to the known

  1. The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region.

    Science.gov (United States)

    Kreikemeyer, Bernd; Nakata, Masanobu; Köller, Thomas; Hildisch, Hendrikje; Kourakos, Vassilios; Standar, Kerstin; Kawabata, Shigetada; Glocker, Michael O; Podbielski, Andreas

    2007-12-01

    Many Streptococcus pyogenes (group A streptococcus [GAS]) virulence factor- and transcriptional regulator-encoding genes cluster together in discrete genomic regions. Nra is a central regulator of the FCT region. Previous studies exclusively described Nra as a transcriptional repressor of adhesin and toxin genes. Here transcriptome and proteome analysis of a serotype M49 GAS strain and an isogenic Nra mutant of this strain revealed the complete Nra regulon profile. Nra is active in all growth phases tested, with the largest regulon in the transition phase. Almost exclusively, virulence factor-encoding genes are repressed by Nra; these genes include the GAS pilus operon, the capsule synthesis operon, the cytolysin-mediated translocation system genes, all Mga region core virulence genes, and genes encoding other regulators, like the Ihk/Irr system, Rgg, and two additional RofA-like protein family regulators. Surprisingly, our experiments revealed that Nra additionally acts as a positive regulator, mostly for genes encoding proteins and enzymes with metabolic functions. Epidemiological investigations revealed strong genetic linkage of one particular Nra-repressed regulator, Ralp3 (SPy0735), with a gene encoding Epf (extracellular protein factor from Streptococcus suis). In a serotype-specific fashion, this ralp3 epf gene block is integrated, most likely via transposition, into the eno sagA virulence gene block, which is present in all GAS serotypes. In GAS serotypes M1, M4, M12, M28, and M49 this novel discrete genetic region is therefore designated the eno ralp3 epf sagA (ERES) pathogenicity region. Functional experiments showed that Epf is a novel GAS plasminogen-binding protein and revealed that Ralp3 activity counteracts Nra and MsmR regulatory activity. In addition to the Mga and FCT regions, the ERES region is the third discrete chromosomal pathogenicity region. All of these regions are transcriptionally linked, adding another level of complexity to the known

  2. A multilevel prediction of physiological response to challenge: Interactions among child maltreatment, neighborhood crime, endothelial nitric oxide synthase gene (eNOS), and GABA(A) receptor subunit alpha-6 gene (GABRA6).

    Science.gov (United States)

    Lynch, Michael; Manly, Jody Todd; Cicchetti, Dante

    2015-11-01

    Physiological response to stress has been linked to a variety of healthy and pathological conditions. The current study conducted a multilevel examination of interactions among environmental toxins (i.e., neighborhood crime and child maltreatment) and specific genetic polymorphisms of the endothelial nitric oxide synthase gene (eNOS) and GABA(A) receptor subunit alpha-6 gene (GABRA6). One hundred eighty-six children were recruited at age 4. The presence or absence of child maltreatment as well as the amount of crime that occurred in their neighborhood during the previous year were determined at that time. At age 9, the children were brought to the lab, where their physiological response to a cognitive challenge (i.e., change in the amplitude of the respiratory sinus arrhythmia) was assessed and DNA samples were collected for subsequent genotyping. The results confirmed that complex Gene × Gene, Environment × Environment, and Gene × Environment interactions were associated with different patterns of respiratory sinus arrhythmia reactivity. The implications for future research and evidence-based intervention are discussed.

  3. Effects of Different Types of Electroacupuncture on Expression of High Mobility Group Box Pro-tein-1 and Interleukin-10 in Hippocampus of APP/PS1 Mice%不同电针法对APP/PS1小鼠海马高迁移率族蛋白B1和白细胞介素-10表达的影响

    Institute of Scientific and Technical Information of China (English)

    赵江豪; 姚海江; 王远征; 卢梦晗; 李昱颉; 宋萌; 杨利娟; 刘俊彤; 李志刚

    2018-01-01

    high mobility group box pro-tein-1 (HMGB1) and interleukin-10 (IL-10) in hippocampus were measured with immunohistochemistry and Western blotting. Results The escape latency shortened inEA groups compared with that of the model group since the fourth day of Morris water maze training (P0.05). The ex-pression of HMGB1 decreased (P<0.05) and the expression of IL-10 increased (P<0.05) in EA groups com-pared with that of the model group, and HMGB1 decreased more and IL-10 increased more in the music-EA group than in the impulse-EA group (P<0.05), according to the measurement in immunohistochemistry, not in Western blotting. Conclusion Both impulse-EA and music-EA based on Tongdu Qishen can promote the recovery of the learning and memory in APP/PS1 mice, and music-EA may do more effectively in inhibition of pro-inflammatory factors and promotion of the anti-inflammatory factors.

  4. Factors responsible for subclinical mastitis in cows caused by Staphylococcus chromogenes and its susceptibility to antibiotics based on bap, fnbA, eno, mecA, tetK, and ermA genes.

    Science.gov (United States)

    Bochniarz, M; Adaszek, Ł; Dzięgiel, B; Nowaczek, A; Wawron, W; Dąbrowski, R; Szczubiał, M; Winiarczyk, S

    2016-12-01

    The aim of this study was to recognize selected factors of virulence determining the adhesion of Staphylococcus chromogenes to cows' udder tissues in subclinical mastitis and to evaluate the susceptibility of this pathogen to antibiotics. The subjects of the study were 38 isolates of Staph. chromogenes from 335 samples of milk from cows with subclinical coagulase-negative staphylococci mastitis. Somatic cell count ranged between 216,000 and 568,000/mL of milk (average 356,000/mL of milk). We confirmed the ability to produce slime in 24 isolates (63.2%), and the ability to produce protease in 29 isolates (76.3%). In each slime-producing isolate, the bap gene was not found, and the fnbA and eno genes were not detected. In vitro tests showed that ceftiofur had the highest effectiveness against Staph. chromogenes (89.5% of susceptible isolates). Minimum inhibitory concentrations ranged from 0.06 to 2µg/mL for susceptible isolates. The minimum concentrations required to inhibit growth of 90 and 50% of the isolates for ceftiofur were at or below the cutoffs recommended by the Clinical and Laboratory Standards Institute (2 and 0.06µg/mL, respectively). A significant percentage of the isolates were susceptible to other β-lactam antibiotics: amoxicillin with clavulanic acid (84.2%) and ampicillin (81.6%). The lowest effectiveness among β-lactams was for penicillin (73.7% of susceptible isolates), and the minimum inhibitory concentration for penicillin ranged from chromogenes (71.1 and 63.2% of susceptible isolates, respectively). The genes tetK (6 isolates) and ermA (1 isolate) were also detected. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  5. Teine muutuva keele ettekandepäev / Helle Metslang, Ilona Tragel

    Index Scriptorium Estoniae

    Metslang, Helle, 1950-

    2007-01-01

    Teisest muutuva keele ettekandepäevast Tartus, 3.11.2006. Muutuva keele ettekandepäevi korraldab ETF-i kahe grandiprojekti töörühm: "Eesti keele grammatilise süsteemi muutumine ja selle põhjused : keelemuutuste analüüs kasutuskontekstis" (Tartu Ülikool, grandihoidja Ilona Tragel) ja "Eesti keele grammatika ja sõnavara dünaamika 1990-2000. aastatel" (Tallinna Ülikool, grandihoidja Helle Metslang)

  6. Iraagi väitel tuleb iga teine terrorist Süüriast / Igor Taro

    Index Scriptorium Estoniae

    Taro, Igor

    2007-01-01

    Bagdadis elurajooni turul hukkus enesetapurünnakus 130 inimest. Iraagi kõrge valitsusametniku sõnul tuleb 50% terrorist üle Süüria piiri. Iraagi peaminister Nuri al-Maliki arvamus. Vt. samas: Mässulised leiavad tuge internetist

  7. Adam Olearius ja kultuuriline Teine teekonnal Oktsidendist Orienti / Aigi Heero, Maris Saagpakk

    Index Scriptorium Estoniae

    Heero, Aigi, 1971-

    2013-01-01

    Erinevate rahvaste kujutamisest 1656. aastal ilmunud Adam Oleariuse tuntud varauusaegses reisikirjas "Vermehrte Newe Beschreibung Der Muscowitischen vnd Persischen Reyse". Eestlaste kujutamisest võrdluses teiste rahvastega

  8. Toimetaja on autori teine, täpsem silmapaar / Reili Argus

    Index Scriptorium Estoniae

    Argus, Reili, 1967-

    2010-01-01

    Intervjuu Tallinna Ülikooli eesti keele ja kultuuri instituudi direktori, eesti keele dotsendi Reili Argusega Tallinna Ülikooli referent-toimetaja bakalaureuseõppekavast ja keeletoimetaja magistriõppekavast

  9. "Sa oled mul teine" : teisesusest eesti kultuuri eneseanalüüsis / Aare Pilv

    Index Scriptorium Estoniae

    Pilv, Aare, 1976-

    2008-01-01

    Kolmest eesti kultuuri eneseanalüüsi kontseptsioonist: "katkestus" (Hasso Krull), "eksistentsialistlik absurdiprojekt" (Jaan Undusk), "enesekolonisatsioon" (Tiit Hennoste). Poleemikat T. Hennoste enesekolonisatsiooni teooriaga (olulisim tekst selle kohta on 2006. a. avaldatud artikkel "Noor-Eesti kui lõpetamata enesekoloniseerimisprojekt"). Sisaldab bibliograafiat. Autorist lk. 249

  10. Tekst ja subjekt. Tartu koolkonna teine põlvkond / Marek Tamm

    Index Scriptorium Estoniae

    Tamm, Marek, 1973-

    1997-01-01

    Arvustus: Lotman, Mihhail. Mandelshtam i Pasternak : popõtka kontrastivnoi poetiki. Tallinn : Aleksandra, 1996. (Biblioteka zhurnala Tallinn); Torop, Peeter. Dostojevski : istorija i ideologija. Tartu : Tartu Ülikooli Kirjastus, 1997

  11. Teine katse: Tallinna Muusikakeskooli, Tallinna Balletikooli ja G. Otsa nimelise Tallinna Muusikakooli uus hoone / Tomomi Hayashi

    Index Scriptorium Estoniae

    Hayashi, Tomomi, 1971-

    2011-01-01

    Žürii liige koolide ühishoone rahvusvahelisest arhitektuurivõistlusest, žürii kommentaarid ja ettepanekud premeeritud kolmele tööle. 1. preemia pälvis töö "Vaikuse aed" (Thomas Pucher jt. / Atelier Thomas Pucher ZT GmbH, Austria), 2. preemia töö "Octopus" (Andrus Mark, Emil Urbel, Aleksandr Zverev / AB Emil Urbel), 3. koha töö "Pausid ja poosid" (Jaan Tiidemann, Tõnis Arjus, Eve Komp / Ninja Stuudio). Jarmo Kauge intervjuu Thomas Pucheriga

  12. El cuento americano: Güeno-mapu

    Directory of Open Access Journals (Sweden)

    Gabriel Carvajal

    1966-02-01

    Full Text Available Helechos. Susurran como el abanico del pavo real. Ahí están. En grupos o hileras sobre la huella rojiza que va al caserío. Junto a la selva. Lejos, adentro, a donde solo llegan a ramonear los vahuales. En la traba de pellines, ulmos y araucarias, rosal silvestre y zarza levantada en altura de lianas y cañaveral.

  13. El niño, oscilación del sur (ENOS, con relación a la transmisión de malaria, densidad y paridad de An. albimanus y An. darlingi (diptera: culicidae en dos regiones de Colombia

    Directory of Open Access Journals (Sweden)

    Ricardo Mantilla

    2000-02-01

    Full Text Available

    Una fuerte asociación ha sido registrada entre los incrementos en la transmisiónas forzadas por el fenómeno El Niño. Entre las posibles explicaciones para esta asociación está el efecto del clima sobre la dinámica de población de vectores, por ejemplo, generando cambios en la densidad y en las tasas de sobrevivencia de la población, y en la cantidad y calidad de sitios de reproducción, como también a través de cambios en el período de incubación extrínseco del parásito dentro del vector. Con el propósito de evaluar la influencia de ENOS sobre la densidad y paridad de Anopheles albimanus y An. darlingi, y su relación con la transmisión de malaria, se realizó un estudio longitudinal en Chocó y Casanare. En cada área se seleccionaron dos localidades y se realizaron muestreos entomológicos durante una semana cada mes, desde finales de 1997 hasta 1999. Este período cubrió los eventos El Niño 1997 -1998 y La Niña 1998 - 2000. En las localidades de Chocó se observó un importante incremento en el número de casos de malaria durante el evento ENOS, asociado con la temperatura. No se observó una asociación estadísticamente significativa entre los casos de malaria y la precipitación o la humedad relativa. La relación entre el incremento de malaria durante El Niño y las variables entomológicas no fue evidente. La densidad y paridad de ambas especies de vectores fluctuaron durante el período de estudio y no se encontró una asociación significativa entre estas variables y los factores climáticos como la temperatura. La precipitación y la humedad relativa. La densidad de larvas de An. albimanus fluctuó durante el estudio, y mostró altas densidades alrededor de julio - agosto. No se observó asociación entre la densidad de larvas con la densidad de adultos o las variables climáticas. El efecto del evento ENOS sobre las poblaciones de vectores

  14. Composición y abundancia de artrópodos epígeos del Parque Nacional Llanos de Challe: impactos del ENOS de 1997 y efectos del hábitat pedológico Abundance and composition of epigean arthropods from Llanos de Challe National Park: impacts of ENSO-1997 and effects of the pedological habitat

    Directory of Open Access Journals (Sweden)

    JORGE CEPEDA-PIZARRO

    2005-12-01

    Full Text Available Mediante el uso de trampas de intercepción, se estudió el efecto de la precipitación y el tipo de hábitat sobre la denso-actividad del ensamble de artrópodos epígeos _en particular Tenebrionidae_ del Parque Nacional Llanos de Challe. Este parque está ubicado en el desierto costero transicional de Chile, en la III Región de Atacama. Los muestreos se realizaron durante tres días por mes (septiembre, octubre y diciembre en el período de máxima actividad biológica del sistema y en los años 1989 (año no-ENOS seco, 1997 (año ENOS intenso y 2000 (año no-ENOS húmedo. En las comparaciones se utilizaron dos sitios pedológicamente contrastantes: un hábitat dunario costero y un hábitat pedregoso interior. Se registró la presencia de 15 órdenes de Arthropoda. Los hexápodos constituyeron > 95 % del total de los especímenes capturados (9.065 individuos, siendo Collembola (36,1 % y Coleoptera (29,8 % los órdenes con mayor representatividad numérica. El número de órdenes con representantes activos varió ligeramente entre años: 13 órdenes se registraron en 1989 (44 % de precipitación bajo el promedio, 15 en 1997 (443 % sobre el promedio y 11 en 2000 (52 % sobre el promedio. El efecto del evento ENOS se reflejó claramente en la contribución numérica en la mayoría de los taxa registrados. Aunque más sutilmente, este efecto también se reflejó en la composición del ensamble de taxa dominantes y entre hábitat pedológicos, particularmente con Tenebrionidae y Formicidae. Especialmente en las dunas costeras, Tenebrionidae dominó claramente el ensamble de artrópodos epígeos, siendo Gyriosomus Guérin-Méneville el género más diverso y abundante. La dominancia de Gyriosomus plantea un conjunto de interrogantes respecto de su nivel de endemismo, diversidad y distribución de sus especies, y el papel funcional de estas en el ecosistema estudiadoBy using pitfall traps, the effects of rainfall and habitat type on the denso

  15. Popist Pärdini, Koreast Eestini : Teine festivalikiri maailma praeguse hetke filmipealinnast Cannes'ist / Jaak Lõhmus

    Index Scriptorium Estoniae

    Lõhmus, Jaak

    2001-01-01

    Cannes'i filmifestivali avafilmist, milleks on Baz Luhrmanni muusikal "Moulin Rouge" : Ameerika Ühendriigid 2001. Ka Eestiga seotud seikadest : võistlusprogrammis on prantsuse mängufilm, mille muusika on Arvo Pärdilt, žüriisse kuulub Jaan Ruus ja P. Simmi "Head käed" on filmiturul huvi äratanud

  16. Space tourism; next goal of rocketry; Uchu eno ryokaku yuso

    Energy Technology Data Exchange (ETDEWEB)

    Inatani, Y. [Institute of the Space and Astronautical Science,Tokyo (Japan)

    2000-01-05

    As to the space travel to be conducted as business, the paper presented the results of the study being made to extract subjects. The single-stage space transport system which can be completely reused is said to be realized by much more weight reduction in airframe structure and improvement of propulsion performance, judging from the present technology standard. The reason the present form of rocket has to be reformed is that the cost needed to put a load of 1kg into orbit around the earth is higher in order of 1 million yen than those of other vehicles. Because the rocket is disposable, it is difficult to make an enormous cost reduction, and therefore, it is necessary to realize the same operation form as that adopted to airplane. The price of tickets for space travel rationally determined by the demand side is between 1.5-2.0 million yen. The annual sales are estimated at approximately 1.3 trillion yen on the market. When calculating this as business, it is necessary to reduce the transportation cost from 1,000,000 yen to 90,000-10,000 yen. A conceptual design was made of a space travel standard airframe 'Kanko-maru.' (NEDO)

  17. Meedikud loodavad rahumeelsele leppele / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Tervishoiutöötajate kutseliitude esindajad võtsid väljakuulutatud streigihoiatuse maha lootuses, et kokkulepe meditsiinitöötajate miinimumtunnitasu osas võib sõlmida rahulikul teel. Arvamust avaldab Haiglate Liidu juhatuse esimees Urmas Sule

  18. President istutas Torisse tamme / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    President Toomas Hendrik Ilves osales Toris Eesti sõjameeste mälestuskirikus traditsioonilisel vabadusvõitluse mälestusele pühendatud jumalateenistusel ja istutas kiriku kõrvale prominentide parki tamme. Varem on sellesse parki tammepuu istutanud president Arnold Rüütel, 20. augusti klubi, endine kaitseväe juhataja Aleksander Einseln ja peapiiskop Andres Põder

  19. President Ilves kohtus õpilaste ja kaitseliitlastega / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    President Toomas Hendrik Ilves külastas mitteametlikul visiidil 9.11.2007 Pärnu Sütevaka humanitaargümnaasiumi ja Kaitseliidu Pärnumaa malevat. Sütevaka humanitaargümnaasiumis kuulas president muusikakanali MTV Europe parima uustulnuka auhinna võitnud poistebändi Bedwetters esinemist ning osales lõpuklassi ühiskonnaõpetuse tunnis, rääkides kooli loosungist "Vabadus, loovus, vastutus" inspireeritult vabadusest ja vastutusest

  20. Feminismo e/no pós-colonialismo

    Directory of Open Access Journals (Sweden)

    Deepika Bahri

    2013-11-01

    Full Text Available http://dx.doi.org/10.1590/S0104-026X2013000200018 Neste artigo, a autora discorre sobre a relação entre feminismo e pós-colonialismoa partir dos conceitos-chave nos estudos pós-coloniais, salientando as premissas, os métodose as tensões dessa aliança, bem como os desafios entre os feminismos “ocidental” e “póscolonial”no contexto da globalização.

  1. Influence of spectral solar radiation to the generating power of photovoltaic module; Taiyo denchi shutsuryoku eno taiyoko supekutoru eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Minaki, S.; Ishihara, Y.; Todaka, T.; Harada, K. [Doshisha University, Kyoto (Japan); Oshiro, T.; Nakamura, H. [Japan quality Assurance Organization, Tokyo (Japan)

    1997-11-25

    As to the influence of spectral solar radiation to generating power of solar cells, a study was conducted from the aspects of season, time zone, intensity of solar radiation, etc. In the study, spectral responsive variation correction coefficients were introduced as evaluation values expressing the influence of spectral solar radiation. For the spectral distribution, an all sky spectral pyranometer by wavelength was used, and data were used which were obtained in the measurement in experimental facilities of the solar techno center. Concerning solar cell relative spectral sensitivity values, used were relative spectral sensitivity values of monocrystal and amorphous standard solar cells to the short-circuit current. Spectral response variation correction coefficients are coefficients correcting variations in conversion efficiency of solar cells due to changes in the spectral distribution. The changes of spectral responsive variation correction coefficients were studied using data obtained during April 1994 and March 1996. As a result, it was found that the coefficients showed large changes in summer and small ones in winter and that amorphous solar cells indicate this trend conspicuously. 3 refs., 6 figs., 3 tabs.

  2. Töösuhetest tulenevate tööandja huvide kaitsmine : [bakalaureusetöö] / Alina Demenštein ; Õigusinstituut ; juhendaja: Mall Gramberg

    Index Scriptorium Estoniae

    Demenštein, Alina

    2001-01-01

    Töölepingu olemusest, sõlmimine, täitmine, poolte õigused ja kohustused, kollektiivleping, töölepingu muutmine, töötaja konfidentsiaalsuskohustus, ärisaladus, distsiplinaarkaristused, materiaalne vastutus, töölepingu lõpetamine

  3. Kandungan kolesterol, High Density Lipoprotein (HDL dan low density lipopro-tein (LDL darah burung puyuh dengan pemberian aditif cair buah naga merah

    Directory of Open Access Journals (Sweden)

    Khabib Arrosichin

    2016-04-01

    Full Text Available The study aims to determine, assess and evaluate the content of cholesterol, high density lipoprotein (HDL and Low Density Lipoprotein (LDL blood quail which were treated with liquid additive red dragon fruit. Materials used in the study were 200 female quails aged 14 days with an average weight of 13.61 ± 0.49 g. Ration composed by metabolizable energy content of ± 3000 kcal/kg and ± 20% of crude protein. The study consisted of 4 treatments and 5 replications. The treatments were (T0: without addition of liquid red dragon fruit (control; T1: addition of 5 ml liquid red dragon fruit twice a day; T2: addition of 5 ml liquid red dragon fruit once a day and T3: addition of 5 ml liquid red dragon fruit once in every two days. Blood sampling was performed in EDTA tube at the end of the study. Analysis of samples was carried out in health laboratory Semarang. The results were analyzed using analysis of variance (ANOVA. The study showed that the addition of liquid red dragon fruit additive had no significant effect (P> 0.05 on cholesterol, HDL and LDL of quil’s blood. Keywords: Quail, cholesterol; HDL, LDL, and red dragon fruit

  4. Leedu poliitika ja kultuuri teine hääl : kolme kõlbelise eluloo visand / Leonidas Donskis ; inglise keelest tõlkinud Kaisa Jõgi

    Index Scriptorium Estoniae

    Donskis, Leonidas, 1962-

    2010-01-01

    Leedu liberaalsest rahvuslusest välisleedu õpetlaste: kultuuriloolase Vytautas Kavolise, poliitikateadlase Aleksandras Stromase, kirjandusprofessori ja luuletaja Tomas Venclova elu ja tegevuse kaudu.

  5. Leedu poliitika ja kultuuri teine hääl : kolme kõlbelise eluloo visand / Leonidas Donskis ; [inglise keelest] tõlkinud Kaisa Jõgi

    Index Scriptorium Estoniae

    Donskis, Leonidas, 1962-

    2002-01-01

    Leedu kolme õpetlase, kultuuriloolase Vytautas Kavolise, poliitikateadlase Aleksandras Stromase, kirjandusprofessori ja luuletaja Tomas Venclova, elu ja tegevus. Tõlgitud käsikirjast "The second voice of Lithuanian politics and culture: sketches of three moral biographies"

  6. Tänavu hüppab börs nagu jänes. Aasta teine pool peaks investoreile rõõmsam olema / Romet Kreek

    Index Scriptorium Estoniae

    Kreek, Romet, 1972-

    2011-01-01

    Aasia maaklerfirma CLSA teeb alates 1992. aastast Hongkongis feng shui rikkuseindeksit, kus püütakse prognoosida, mida on saabunud aastal kuude lõikes Hongkongi börsil oodata ning milliste sektorite käsi võiks teistest paremini käia. Graafik

  7. Protein (Cyanobacteria): 464885 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available tein Nos7524_5075 Nostoc sp. PCC 7524 MSLSYDISSILNLLRSLPSTELRTVKEEIDSILKERGTTIRIPDPFKIVPAQVVLKDSNLEESTSEVKLEEEYQQINEDISEPSGVLNLSSIKDATDNKAEKKEAIQEIPRPLGIWKGKVEISEDFYETTNDILSEFGIEE ...

  8. Risk communication. Risk studies in social science; Risk communication. Risk kenkyu eno shakai kagakuteki approach

    Energy Technology Data Exchange (ETDEWEB)

    Asami, M. [Keio Univ., Tokyo (Japan)

    1995-11-05

    It is recently, that is, in and after the 1980s that socio-scientific approaches began to be made to studies on `risk`. It started to be made clear that the progress of the scientific technology does not necessarily bring about good news to man, and obvious estrangement has begun to appear between scientific evaluation and social evaluation of risk. The subject of risk communication (RC) study is to tackle a proposition whether the estrangement will continue to exist as estrangement or the estrangement can be made smaller by any means. This paper explains the recent trend of the study. For example, as for how each individual thinks about risk, that is, the risk perception, a new framework is trially being constructed by introduction of the quantitative method using psychometrics. A duty of RC is to serve to bridge the gap between scientific technology and society which are controlled by values which are incompatible with each other. Therefore, RC will be more and more important. 15 refs., 1 fig., 2 tabs.

  9. Application of wavelet transform to seismic data; Wavelet henkan no jishin tansa eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Nakagami, K; Murayama, R; Matsuoka, T [Japan National Oil Corp., Tokyo (Japan)

    1996-05-01

    Introduced herein is the use of the wavelet transform in the field of seismic exploration. Among applications so far made, there are signal filtering, break point detection, data compression, and the solution of finite differential equations in the wavelet domain. In the field of data compression in particular, some examples of practical application have been introduced already. In seismic exploration, it is expected that the wavelet transform will separate signals and noises in data in a way different from the Fourier transform. The continuous wavelet transform displays time change in frequency easy to read, but is not suitable for the analysis and processing large quantities of data. On the other hand, the discrete wavelet transform, being an orthogonal transform, can handle large quantities of data. As compared with the conventional Fourier transform that handles only the frequency domain, the wavelet transform handles the time domain as well as the frequency domain, and therefore is more convenient in handling unsteady signals. 9 ref., 8 figs.

  10. Spatial autocorrelation method using AR model; Kukan jiko sokanho eno AR model no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, H; Obuchi, T; Saito, T [Iwate University, Iwate (Japan). Faculty of Engineering

    1996-05-01

    Examination was made about the applicability of the AR model to the spatial autocorrelation (SAC) method, which analyzes the surface wave phase velocity in a microtremor, for the estimation of the underground structure. In this examination, microtremor data recorded in Morioka City, Iwate Prefecture, was used. In the SAC method, a spatial autocorrelation function with the frequency as a variable is determined from microtremor data observed by circular arrays. Then, the Bessel function is adapted to the spatial autocorrelation coefficient with the distance between seismographs as a variable for the determination of the phase velocity. The result of the AR model application in this study and the results of the conventional BPF and FFT method were compared. It was then found that the phase velocities obtained by the BPF and FFT methods were more dispersed than the same obtained by the AR model. The dispersion in the BPF method is attributed to the bandwidth used in the band-pass filter and, in the FFT method, to the impact of the bandwidth on the smoothing of the cross spectrum. 2 refs., 7 figs.

  11. Water supply method to the fuel cell cooling water system; Nenryo denchi reikyakusuikei eno kyusui hoho

    Energy Technology Data Exchange (ETDEWEB)

    Urata, T. [Tokyo (Japan); Nishida, S. [Tokyo (Japan)

    1996-12-17

    The conventional fuel cell has long cooling water piping ranging from the fuel cell exit to the steam separator; in addition, the supply water is cooler than the cooling water. When the amount of supply water increases, the temperature of the cooling water is lowered, and the pressure fluctuation in the steam separator becomes larger. This invention relates to the water supply method of opening the supply water valve and supplying water from the supply water system to the cooling water system in accordance with the signal of the level sensor of the steam separator, wherein opening and closing of the supply valve are repeated during water supply. According to the method the pressure drop in every water supply becomes negligibly small; therefore, the pressure fluctuation of the cooling water system can be made small. The interval of the supply water valve from opening to closing is preferably from 3 seconds to 2 minutes. The method is effective when equipment for recovering heat from the cooling water is installed in the downstream pipeline of the fuel cell. 2 figs.

  12. Sorption mechanism of solvent vapors to coals; Sekitan eno yobai joki no shuchaku kiko no kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Shimizu, K.; Takanohashi, T.; Iino, M. [Tohoku University, Sendai (Japan). Institute for Chemical Reaction Science

    1996-10-28

    With an objective to clarify the interactions between micropore structure of coal and solvent reagents, a sorption experiment was carried out under solvent saturated vapor pressure. Low-volatile bituminous coal, Pocahontas No. 3 coal, has the aromatic ring structure developed, and makes solvent more difficult to diffuse into coal, hence sorption amount is small. Methanol has permeated since its polarity is high. High-volatile bituminous coal, Illinois No. 6 coal, makes solvent penetrate easily, and the sorption amount was large with both of aromatic and polar solvents. Since brown coal, Beulah Zap coal, contains a large amount of oxygen, and hydrogen bonding is predominant, sorption amount of cyclohexane and benzene having no polarity is small. Methanol diffuses while releasing hydrogen bond due to its polarity, and its sorption amount is large. A double sorption model is available, which expresses the whole sorption amount as a sum of physical sorption amount and amount of permeation into coal. This model was applied when it explained successfully the sorption behavior of the solvents relative to coals, excepting some of the systems. However, also observed were such abnormal behavior as sorption impediment due to interactions between coal surface and solvents, and permeation impediment due to hydroxyl groups inside the coals. 1 ref., 10 figs., 2 tabs.

  13. Adjustment of muscle function to flight in bats; Komori no kinkino no hiko eno tekio

    Energy Technology Data Exchange (ETDEWEB)

    Yamashita, M. [Institute of the Space and Astronautical Science,Tokyo (Japan); Choi, I.H.

    1999-12-05

    This paper outlines the muscle of bats that generates a motive force for flight. The weight of the muscle is less compared with that of birds. The energy required for flight is twice as much as that for running. Conversely, in view of metabolic cost (transporting cost) for moving a unit mass for a unit distance, the transporting cost of bats for flying is one fifth. The acquisition of this flight ability through evolution can be inferred from the fossils of reptiles. Bats, having a stream-lined body shape and a small body mass, are capable of efficient flight. A fast durable flight is possible by having the pectoral muscle constituted of speed muscles of oxidation/glycolysis muscle fiber, a well-developed oxygen transporting system, the arrangement around the capillary of mitochondria and fat grains that are cell organs for producing energy, and a high-density contact between the capillary and the muscle fiber. The muscle functions at low body temperature and imparts adaptability to hibernation with the body temperature lowered. The flight is controlled by the cycle and synchronized with this biological clock, optical cycle and change in temperature. (NEDO)

  14. Resting Tension Affects eNOS Activity in a Calcium-Dependent Way in Airways

    Directory of Open Access Journals (Sweden)

    Paschalis-Adam Molyvdas

    2007-03-01

    Full Text Available The alteration of resting tension (RT from 0.5 g to 2.5 g increased significantly airway smooth muscle contractions induced by acetylcholine (ACh in rabbit trachea. The decrease in extracellular calcium concentration [Ca2+]o from 2 mM to 0.2 mM reduced ACh-induced contractions only at 2.5 g RT with no effect at 0.5 g RT. The nonselective inhibitor of nitric oxide synthase (NOS, NG-nitro-L-arginine methyl ester (L-NAME increased ACh-induced contractions at 2.5 g RT. The inhibitor of inducible NOS, S-methylsothiourea or neuronal NOS, 7-nitroindazole had no effect. At 2.5 g RT, the reduction of [Ca2+]o from 2 mM to 0.2 mM abolished the effect of L-NAME on ACh-induced contractions. The NO precursor L-arginine or the tyrosine kinase inhibitors erbstatin A and genistein had no effect on ACh-induced contractions obtained at 2.5 g RT. Our results suggest that in airways, RT affects ACh-induced contractions by modulating the activity of epithelial NOS in a calcium-dependent, tyrosine-phosphorylation-independent way.

  15. Riik hakkab Via Baltica maanteed neljarealiseks joonistama / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 25. sept. lk. 2. Pärnu maavalitsuse ja maanteeameti eestvedamisel alustatakse Tallinnast Iklani kulgeva Via Baltica liiklussoone teemaplaneeringut, mille järgi tuleb Pärnumaal leida lahendused Are ja Pärnu ümbersõidule ning Nurme õgvendusele. Arvamust avaldab Are vallavanem Jaanus Männik

  16. REŠEVANJE DELOVNIH SPOROV S SODIŠČU PRIDRUŽENO MEDIACIJO

    OpenAIRE

    Goričan Podbrežnik, Sabina

    2016-01-01

    Za človeško družbo je značilno, da se v njej pojavljajo spori.Enega izmed načinov reševanja sporov predstavlja mediacija kot glavni in tipični predstavnik alternativnega reševanja sporov. S sprejetjem Zakona o mediaciji v civilnih in gospodarskih zadevah se je Slovenija pridružila državam, ki imajo zakonsko urejeno mediacijo. Na podlagi Zakona o alternativnem reševanju sodnih sporov pa je mediacija postala tudi del rednega poslovanja slovenskih sodišč. Sodišču pridružena mediacija je edini po...

  17. Model Skinner Operant Conditioning automata i bionički naučeno upravljanje robota

    OpenAIRE

    Cai, Jianxian; Hong, Li; Cheng, Lina; Yu, Ruihong

    2016-01-01

    Fuzzy Skinner Operant Conditioning Automaton (FSOCA) sastavljen je na temelju Operant Conditioning mehanizma primjenom teorije neizrazitih skupova. Osnovno obilježje automata FSOCA je sljedeće: neizraziti rezultati stanja pomoću Gausove funkcije koriste se kao skupovi neizrazitog stanja; neizrazita pravila preslikavanja (fuzzy mapping rules) kod fuzzy-conditioning-operacije zamjenjuju stohastičke "conditioning-operant" skupove preslikavanja. Stoga se automat FSOCA može koristiti za opisivanje...

  18. Pärnu võimukoalitsioon kutsub pataljoni toetama / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Pärnu võimuerakonnad kutsuvad linna ja maakonna elanikke kõnekoosolekule, kus nad soovivad avaldada nördimust kaitseministeeriumi plaani üle sulgeda selle aasta lõpul Pärnu üksik-jalaväepataljon

  19. Maavanema avaldus surus linnavõimu kaitsesse / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Pärnu maavanem Toomas Kivimägi kritiseeris linnavalitsust Via Baltica Pärnu ümbersõidu projekti venimise pärast. Linnapea Mart Viisitamm ei pea avaldust põhjendatuks. Vt. samas lk. 15: Mart Viisitamm. Demokraatia või diktatuur - selles on küsimus

  20. Pidupäevaüritused olid Eestile ilus kingitus / Andris Tammela, Eno-Gerrit Link

    Index Scriptorium Estoniae

    Tammela, Andris

    2008-01-01

    Ülevaade EV 90. aastapäeva juubeliüritusest Pärnus. Vt. samas: Vabariigi juubel värvis Pärnu sinimustvalgeks; Head emotsioonid Eesti aastapäevalt: Are vallavanem Jaanus Männik, Euroopa Parlamendi liige Marianne Mikko, Pärnu linnavolikogu liikmed Valter Parve ja Raul Sarandi ja Pärnu maavanem Toomas Kivimägi

  1. Abilinnapea tõrjub opositsiooni süüdistusi / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Pärnu linnavalitsuse rahanduse abilinnapea Vambo Talu ei nõustu IRL poliitikute süüdistustega, et linna viletsas finantsolukorras on süüdi volikogu loata eelmise aasta eelarvest kärbitud 116 miljonit krooni

  2. Streigi saatus selgub täna lõunaks / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Riiklik lepitaja Henn Pärn tegi viimase kompromissettepaneku. Juhul, kui ametiühingute, tööandjate ja lepitaja kohtumisel osalised seda heaks ei kiida, alustatakse hoiatusstreigiga. Vt. samas: Pärnu streikivate bussijuhtide pöördumine linnarahva poole

  3. Estonia juhti kahtlustatakse ettevõtte varade omastamises / Ester Vilgats, Eno-Gerrit Link, Teet Roosaar

    Index Scriptorium Estoniae

    Vilgats, Ester

    2008-01-01

    Endine Pärnu linnapea, taastusravikeskuse Estonia pikaaegne juht Vello Järvesalu peeti kinni kahtlustatuna ettevõtte varade omastamises suures ulatuses. Kommenteerivad: Mart Viisitamm, Jane Mets ja Ahti Kõo

  4. Päästjatele tuli mullu tuhat väljakutset / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Pärnumaa päästeteenistus sai 2005. a. kõige rohkem väljakutseid Pärnusse ning Via Baltica äärde jäävatesse Pärnumaa valdadesse ja Vändrasse. Kaart: Pärnumaa päästeteenistuste väljasõidud omavalitsustesse 2005

  5. Pärnu linnavalitsuse hinnang esimesele valitsemisaastale / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Ajaleht esitas Pärnu linnajuhtidele kolm küsimust. Vastasid linnapea Mart Viisitamm, abilinnapea majanduse valdkonnas Raul Sarandi, abilinnapea arengu ja planeerimise valdkonnas Mart Alliku, abilinnapea kultuuri, spordi ja sotsiaalvaldkonnas Margus Tammekivi ja linnavalitsuse liige turvalisuse valdkonnas Simmo Saar. Arvamust avaldavad Reformierakonna Pärnu piirkonna esimees Väino Hallik ja SDE esindaja Pärnu linnavolikogus Valter Parve

  6. Poliitikud ei leidnud staadioni planeeringule uusi lahendusi / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Riigikogu Pärnumaa saadikuteühenduse ja Pärnu linnavõimu esindajate ning spordiseltsi Kalev juhtide nõupidamine Kalevi staadioni ümberehituse käimalükkamiseks lõppes tulemusteta, sest kõiki osapooli rahuldava tulemuseni ei jõutud. Kommenteerivad Mark Soosaar ja Vello Järvesalu

  7. Sotsid said prostitutsiooni ürituse pärast noomida / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Ilmunud ka: Pärnu Postimees 16. märts lk. 3. Pärnu linnavalitsuse kultuuriosakonna peaspetsialist Tair Anton tegi kaubanduskeskuses Port Artur 2 prostitutsiooni vastu vastu allkirju kogunud sotsiaaldemokraatidele loata avaliku ürituse korraldamise eest suulise märkuse

  8. Koalitsioon ei plaani linna juhtimises muudatusi / Ester Vilgats, Eno-Gerrit Link

    Index Scriptorium Estoniae

    Vilgats, Ester

    2008-01-01

    Pärnu koalitsiooninõukogu sõnul pole põhjust linnapea Mart Viisitamme, abilinnapea Simmo Saare ja koalitsiooninõukogu esimehe Vello Järvesalu välja vahetamiseks, kuna kedagi pole süüdi mõistetud

  9. President tunnustab Pärnumaad kümne riikliku autasuga / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Pärnumaalt saavad iseseisvuspäeva puhul riikliku autasu kümme inimest. Vt. samas: Ester Vilgats. Ivar Kaldasaunal on alati kollektiiviga vedanud; Vello Lahtvee kolmkümmend aastat kohtunikuna. Teenetemärgi pälvinute seas on Lääne-Eesti päästekeskuse direktor Ivar Kaldasaun ja Pärnu maakohtu kohtunik Vello Lahtvee. Vt. ka juhtkiri lk. 15: Vilgats, Kalev. Ebaõigluse visa vastupanu. Rüütliristi kavaler Harald Nugiseks jäi taas teenetemärgist ilma

  10. Prospects to solar energy power generation in space. Uchu taiyo hatsuden eno tenbo

    Energy Technology Data Exchange (ETDEWEB)

    Kudo, I. (Electrotechnical Laboratory, Tsukuba (Japan))

    1993-05-01

    Solar energy power generation in space uses large arrays of solar cells developed on a geosynchronous orbit to obtain electric energy, which is transmitted to the earth using microwaves. The idea had already been advocated in 1968, which was followed a decade later by joint discussions done by NASA and DOE. The concept intended to take care of the U.S. power demand by using 60 power plant satellites, each having an output of 5 GW. This expanse of the scale, regarded reasonable even today, calls for the solar cell arrays in space spreading over an area of 10 km [times] 5 km if silicon solar cells with a conversion efficiency of 15% are used, and rectenna on the ground (a received wave converting facility) forming an ellipse of 10 km [times] 13 km (assuming a location at the north latitude of 36[degree]). Although there are a number of problems in the idea such as transportation means to lift construction materials into the space and effect of microwaves on the ionosphere and the ecosystems, the Agency of Industrial Science and Technology organized a 'committee for investigating and studying the space power generation systems' in the fiscal year 1991, and has been moving discussions forward since then. 7 refs., 5 figs.

  11. Application of thermal spray coatings for jet engines. Kokuki sangyo eno yosha no oyo

    Energy Technology Data Exchange (ETDEWEB)

    Muto, Y [All Nippon Airways Co. Ltd., Tokyo (Japan)

    1992-10-31

    Application condition of spray coating on jet engine parts and characteristics of spray reparing process are explained. Spray coating used for jet engine is classified as recovery of dimension, crevice adjustment, improvement of resistance to friction, improvement of fretting resistance and heat resistance. Titanium alloy having better adhesion and acid resistance, is used as coating for dimensional recovery, where as nickel-crome-aluminium coating is used for the improvement of heat resistance of stainless steel, etc. Crevice adjustment coatings are used in rotating parts of jet engines, and they are of two types are; gel-double coating of aluminium, nickel-aluminium, etc., abrasive coating of aluminium oxide. Tungsten carbide and cobalt are used as coatings for the friction improvement. Nickel and indium, etc., are used as fretting resistance coating. Various types of ceramics together with heat resistance steels like HS-188 are used as coating for heat resistance improvement. 4 figs., 3 tabs.

  12. Effect of water pollution on marine organisms; Sekiyu osen no kaiyo seibutsu eno eikyo hyoka

    Energy Technology Data Exchange (ETDEWEB)

    Ogata, M. [Okayama Univ., Okayama (Japan); Fujisawa, K. [Okayama Prefectural Fisheries Experiment Station, Okayama (Japan)

    1997-10-10

    Toxicity of petroleum component to aquatic organisms appears as a result of its deposition onto living organisms followed by its migration into bodies of the organisms, and emergence of toxicity from the migrated component. Effect evaluation processes standing on this viewpoint may include the exposure monitoring or migration monitoring, in which the petroleum component migrated into marine organisms is analyzed and the state of the component concentrated in these organisms is measured, or effect monitoring, in which actions of the petroleum component in the organisms are investigated. The effects of petroleum on aquatic organisms would include the following occurrence: direct fatal toxicity acting on cells and membranes, quasi-fatal toxicity causing death indirectly through feeding actions and abnormal actions, direct coating of oil on surface of organisms, which prevents movability and feeding actions of the organisms and reduces hydrophilicity of plumes and hairs, pollution of living organisms due to migration of carcinogenic aromatic compounds into bodies of the living organisms, and change in species compositions and geographic distribution of living organisms due to change in physico-chemical environment. This paper explains cases of detection and identification of organic sulfur compounds, aromatic compounds, polycyclic aromatic compounds, paraffins, olefins and heavy metals in parametric compounds of petroleum. 20 refs., 4 figs., 3 tabs.

  13. Truncus Arteriosus in a 43 year old Male: Case Report | Ng'eno ...

    African Journals Online (AJOL)

    In truncus arteriosus, the embryologic truncus fails to properly divide in-utero resulting in the pulmonary, aortic and coronary arteries arising from a single ascending portion of this trunk. This condition is usually fatal within the first year of life without correction. Over the past two decades, there has been a dramatic expansion ...

  14. Trial to active seismic while drilling; Jinko shingen wo mochiita SWD eno kokoromi

    Energy Technology Data Exchange (ETDEWEB)

    Tsuru, T; Kozawa, T [Japan National Oil Corp., Tokyo (Japan). Technology Research Center

    1996-10-01

    This paper describes the development of a more stable SWD system with larger energy by adding an artificial seismic source near the bit. SWD is a technique by which the seismic wave generated while drilling of rocks by bit can be observed on the ground surface and the records equivalent to the reverse VSP can be obtained. For this system, a shell with a vibrator was fixed immediately on the bit as a sub-generator, and total energy of usual impact by the bit and vibration by the vibrator was used as a seismic source for SWD. For the seismic wave generation mechanism of this vibrator, the shell was resonated by the magnetostrictive element, and vibration was given to the bit and drilling pipe. When this seismic source is used, only single frequency is obtained for each vibration due to the utilization of resonance of shell. Therefore, the generation patterns should be made, by which wide band energy can be obtained after the interaction. Since the survey was conducted using this bottom hole seismic source at the drilling depth more than 3,000 m, it was necessary to enhance the vibration energy. 2 refs., 2 figs.

  15. Application of EM tomography to detect a buried pipe; EM tomography no maisetsukan tansa eno tekiyorei

    Energy Technology Data Exchange (ETDEWEB)

    Sakashita, S [OYO Corp., Tokyo (Japan)

    1996-10-01

    EM tomography was applied to detect buried pipes. Underground radar exploration method is limited to 10m in depth. Positive use of bored holes is desirable, and in such case, magnetic logging based on the magnetic susceptibility (MS) contrast between buried body and surrounding ground is effective. The primary magnetic field is generated by coil current, and the secondary one is generated by the primary one responding to foreign bodies in the ground. Since the measured primary magnetic field of low frequency within 10Hz can be treated as static magnetic field responding to MS in the ground, it is useful to determine MS distributions. Since the measured magnetic field of high frequency within 100kHz can be treated as induction field responding to conductivity in the ground, it is useful to determine resistivity distributions. The EM tomography which can image both above distributions by using electromagnetic wave in a wide frequency range, was applied to detect buried pipes. The EM tomography could detect an buried foreign body of 3m in diameter at 10m in distance between bored holes. The theoretical equation for analysis was also derived. 5 refs., 9 figs., 1 tab.

  16. Application of wear resistant spraying for diesel engine; Diesel kikan eno taimamo yosha no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Kitajima, Y. [Mitsui Engineering and Shipbuliding Co. Ltd., Tokyo (Japan)

    1999-03-31

    Diesel engines used widely as propelling engines of ships have increasingly been provided with a high output and a high thermal efficiency; their structural members, particularly, the component parts for combustion chambers are therefore used under severe conditions, giving rise to the need of surface treatment and surface reforming of the members. Parts for marine diesel engines are huge, so that the technology applicable to the surface treatment and reforming are limited in point of facility and cost; therefore, most suitable is thermal spraying. This paper primarily discusses, among marine diesel engines, a 2-cycle low-speed engine with a 260-980mm bore used for the main engine of a merchant ship such as a container ship, bulk carrier or a tanker, and a 4-cycle medium-speed engine with a 300-420mm bore used for the main engine of a naval vessel; the paper explains the application status of a thermal spraying technology which is in progress to cope with the high output and high thermal efficiency of the diesel engines, explaining particularly the story of the development and the technological features of the wear resistant thermal spraying, which has been put to practical use, on the cylinder liner and the piston ring of the 4-cycle medium-speed engine. (NEDO)

  17. Application of ECS to a hybrid automobile; `ECS` no hybrid jidosha eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Shimizu, M.; Okamura, T.

    1997-01-30

    This paper describes a simulation for a case when an energy capacitor system (ECS) is mounted in a hybrid electric vehicle in place of secondary batteries. The ECS is electric power storage equipment consisted of capacitors and electronic circuits, and capable of quick charge and discharge at high efficiency. Its energy density per unit weight is about the same as lead-acid batteries, but its output density is greater than any type of batteries. Being free of deterioration due to charge and discharge, its cycle life is almost limitless. In order to suppress heat generation, a switching converter is used to control the output to about 300W. However, internal resistance of an ECS is smaller than in an equivalent secondary battery, and internal loss and heat generation stay as low as 1/5 to 1/10. Discussions have been given on cases of using the ECS in small passenger cars, small buses/trucks, and large buses. Since the ECS can use the stored power to about 94% of the capacity, and its cycle life is sufficiently long, it can operate with charge capacity as small as 1/4 of a lead-acid battery. The weight can also be reduced largely. Regenerative power storage efficiency is high, fuel consumption can be improved, heat generation is less, and no forced cooling is required. 9 refs., 4 figs., 1 tab.

  18. Influence of life line stoppage on building equipments; Lifeline tozetsu no kenchiku setsubi eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Sadohara, S. [Yokohama National University, Yokohama (Japan). Faculty of Engineering

    1995-06-05

    Our daily life is being supported by electricity in various ways. This paper, explains the influence of the stoppage of the electricity, affecting infrastructures, buildings and people`s daily life based on the survey result of a long term electricity stoppage in Hiroshima City in September 1991. This paper also introduces the survey result of emergency generators operation in Japan and in the U.S. Last, the ways and ideas to make lifeline reliability higher in the future are summarized. 1 ref., 11 figs., 7 tabs.

  19. PV glass curtain walls; Kenzai ittaigata taiyo denchi gaiheki no kaihatsu (glass curtain wall eno tekiyo)

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, T.; Iwai, T.; Ouchi, T.; Ito, T.; Nagai, T.; Shu, I. [Kajima Corp., Tokyo (Japan); Arai, T. [Showa Shell Sekiyu K.K., Tokyo (Japan); Ishikawa, N.; Tazawa, K.

    1997-12-20

    Reported in this article are PV (photovoltaic) modules now under development for integration into building walls. First of all, the power generating capability of PV modules and appropriate use of the generated power are studied, and the performance (resistance to fire or incombustibility, strength and durability, appearance and design, and dimensional standardization) that such outer wall materials are to be equipped with are determined. Next, module development, installation technique, computer graphics-aided facade designing, and real size module-using proof test are studied before installability, the power to be generated, and designs are finalized. In the development of modules, design evaluation involves the combining of various kinds of glass, solar cells, back sheets, and fillers, and the importance is confirmed of the prevention of insulation degradation around the modules. As for the methods of installation, the gasket method and aluminum sash method, etc., are tested. In the study of facade design, it is found that various expressions are possible by properly choosing gasket colors, module types, and kinds of glass to cover the openings. 1 ref., 6 figs., 3 tabs.

  20. Waste minimization activities in Toyota Motor Corporation; Haikibutsu teigen eno torikumi jokyo

    Energy Technology Data Exchange (ETDEWEB)

    Taguchi, S.; Yamamoto, N.; Kurai, H. [Toyota Motor Corp., Aichi (Japan)

    1998-07-01

    Toyota`s waste reduction efforts based on a drive for dealing with the source of waste generation are introduced. For the propulsion of the policy for dealing directly with the origin of waste, it is necessary to convert the existing manufacturing processes that give high priority to product quality and low production cost into environmental impact reduction oriented processes. In particular, conversion of materials into those that are easier to recycle, technology for reducing defective products, technology for the completion of recycling within the process involved, and the simplification of all the processes are mentioned. For the cleaning job in the machining process, a detergent enhanced in oil/water separating capability is developed, and this prolongs doubly the detergent replacement period and decreases the waste liquid to a 1/4 or less of what the conventional method produces. For the handling of foundry sand that is yielded in great quantities, a flow classification system is developed to replace the conventional gravitational classifier, and the reusable coarse-grain sand in the collected dust which occupies approximately 70% of the whole waste is now recovered for reuse. In the vehicle body coating process, a newly-developed robot arm type coating system is developed, which halves the coating waste. 2 refs., 9 figs., 1 tab.

  1. Role of biotechnology in future agriculture. Korekarano nogyo to biotechnology eno kitai

    Energy Technology Data Exchange (ETDEWEB)

    Komano, T. (Kyoto Univ., Kyoto (Japan). Faculty of Agriculture)

    1992-09-01

    In comparison with ancient times when everything is handled empirically, biological matter suitable for purposes can be produced and utilized faster and more reliably these days when life science has made a great advance. The advancement is related to new breeding technology and production means, and those means offer the point of contact between biotechnology and agriculture. The application fields of biotechnology are microbiology, cell technology, enzyme technology (bioreactor), and gene engineering. High yield, high content of high value ingredients as foods, adaptability to environment, resistance to disease and insect damage, etc. may be the subjects expected for future agricultural organisms. There may be many areas where biotechnology is related to those organisms, but a discussion is made in this report centering around the problem in breeding. Outlines are given on the applied cases of cell technological method, gene engineering method, and recombinant DNA technology, as well as on gene engineering for plants and animals. 10 refs., 7 figs.

  2. Astronaut suitability requirements and selection process; Uchu hikoshi tanjo eno michi (shishitsu yokyu)

    Energy Technology Data Exchange (ETDEWEB)

    Miyoshi, H. [National Space Development Agency of Japan, Tokyo (Japan)

    1999-10-05

    Manned space activities at National Space Development Agency of Japan and the suitability requirements that an astronaut is supposed to satisfy are described. At the first phase, candidates have to participate in a manned space experiment utilizing a NASA space shuttle and, in 1985, Mori, Mukai, and Doi were selected to be payload specialists. At the second phase, Astronauts Wakata, Doi, and Mori were sent to the mission specialist training course, this being one of the jobs aboard a space shuttle, which was for preparing for the construction and operation of the international space station. In January, 1996, Astronaut Wakata performed extravehicular tool manipulation and so forth, and Astronaut Doi did the same in 1997. The endowments that an astronaut is expected to have include undoubted professionalism, adaptability to branches out of his field, adaptability to a prolonged stay in space, spirit of teamwork and coordination, and ability to perform wide range of duties aboard an international space station. (NEDO)

  3. Projekteerijad asuvad kesklinna võimla kavandit täiendama / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2001-01-01

    Pärnu Vanalinna põhikooli kõrvale kavandatavast võimlast. Võimla eskiisprojektide konkursi võitis (2. koht.) Ott Kadariku ja Mihkel Tüüri töö "Jae-Ja", 3. koha sai Kalle Kadalipu töö "Neo"

  4. Application of essential facility doctrine to transmission grid; Sodenmo eno essential facility no hori no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Maruyama, M. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1997-06-01

    This paper arranges the essential facility doctrine in America and describes it according to each judgment example how the doctrine is applied on the access of electricity supply companies to transmission grids. By applying the result to the situation of Japan, the point at issue occurring in the range of the Anti-Monopoly Law was arranged and investigated when third parties (power generating manufacturers) access the transmission grids of electricity supply companies in the form of wholesale or self transmission in the electricity supply industry of Japan. In the judgment example of America, a transmission grid can be considered as essential facility. The refusal of essential facility application is judged to be illegal only when it aims at keeping and expanding the leading market share without a fair reason. The application of essential facility can be refused when the transmission line is insufficient in capacity, when no necessary technical conditions are satisfied during use, or when the cost generated during use cannot be collected. 18 refs., 7 figs., 2 tabs.

  5. Application of FEM flow analysis to environmental engineering. FEM ryutai kaiseki no kankyo gijutsu eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Higuchi, M; Ando, K; Shimada, S; Umetani, H [Toyota Motor Corp., Aichi (Japan)

    1990-04-25

    In order to apply FEM (finite element method) flow analysis to environmental engineering, the two-dimensional analysis system was developed, and applied to the design of plant ventilation and improvement of an enviromental control equipment. Furthermore, the three-dimensional analysis system was developed to extend its application. Since in a large scale model, no enhancement of a processing capacity was expected even by a supercomputer because of longer I/O times between internal and external memories caused by a small internal memory space, the parallel processing system with multiple external memories was introduced to analyze such models. To achieve more efficient processing, multiple series of renumbering codes were also prepared to optimize the processing order of solvers, elements and nodes. As examples, the improvement of a thermal oxidizer and the ventilation design for a forging plant, and as an example of a three-dimensional large scale model, the flow analysis around a plant were presented. 8 refs., 17 figs., 1 tab.

  6. Application of ceramics to the sliding seat of valve bridge; Valve bridge yodobu eno ceramics tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Matsui, T; Ono, T [Mitsubishi Motors Corp., Tokyo (Japan)

    1997-10-01

    For use in the valve train, using an OHV (over head valve) configuration. of a 4 valve diesel engine for trucks and buses; we developed a valve bridge, a component of a valve train, with a ceramic head that is made of silicon nitride(Si3N4) in contact with a rocker arm in order to reduce cost and improve wear resistance for further diesel engine emissions regulations. In order to evaluate the effect of this valve bridge, RIG tests and durability tests on actual engines were carried out. 7 figs., 2 tabs.

  7. effect of dl-carnitine on tissue content of ad eno ine triphospha te

    African Journals Online (AJOL)

    BSN

    values of 16.2 ± 5.2µ mols lg dry weight for ATP and I 1.7± 2.5 ~11nols/g dry weight for CP in normal (non ischaem1c) myocardial tissue. Continuous perfusion of the heans "ith perfusate contaming 4.9µmols/ml. DL-Carnitine appeared to prevent the lowermg of ATP and CP levels as a result of induction of ischaemia.

  8. Utilization of new materials in prestressed concrete. Shin zairyo no puresutoresuto concrete eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Imai, Y. (Taisei Corp., Tokyo (Japan)); Kato, H. (Building Research Inst., Tokyo (Japan)); Mutsuyoshi, H. (Saitama Univ., Urawa (Japan). Faculty of Engineering)

    1994-03-31

    In this session for the new materials, almost all of them were the presentation on the fiber reinforced plastics (FRP). Based on these facts, the topics of new materials about PC centered around FRP, and consequently it was found that how it was expected as the new materials and how it is being watched with keen interest. Even in FRP, the presentations on the carbon fiber reinforced plastics (CFRP) and aramid fiber reinforced plastics (AFRP) were a little bit more than those on the glass fiber reinforced plastics (GFRP), and therefore they were felt to be predominant. Although the ones about a fundamental study on the material characters of FRP held approximately 70%, as a content, there were 6 presentations on the design and execution of the actual bridges in which FRP was used for the PC take-up materials and the oblique cable of cable-stayed bridges, consequently it was found that FRP did not stay at a study stage, and was already shifting to the actual application stage. However, at the current situation, there were many of experimental ones like the small scale bridges in the golf courses, and furthermore it can not be said that a guideline for the design and execution is sufficiently established. 2 figs.

  9. Sõjaajaloolane Ivika Maidre : "Teine maailmasõda lõpeb meis ehk 200 aasta pärast" = Vojennõi istorik Ivika Maidre : "Vtoraja mirovaja voina zakontshitsja v nas let, mozhet, tsherez 200" / Ivika Maidre ; interv. Erik Kalda

    Index Scriptorium Estoniae

    Maidre, Ivika

    2007-01-01

    Autor arvab, et viimase sõja lõppemine meie hinges võib sündida alles mitmete inimpõlvede järel - niikaua tuleb otsida võimalusi kokku leppida. Ilmunud ka: Severnoje Poberezhje : Subbota, 5. mai 2007

  10. Noor-Eesti enesekoloniseerimisprojekt. Teine osa Olulised kirjandusmõtteviisid . The Self-Colonization Project of Young Estonia. Part II. Modes of Literary Thinking and Relations with Colonialism in Estonian Literature of the beginning of the 20th century

    Directory of Open Access Journals (Sweden)

    Tiit Hennoste

    2012-04-01

    Full Text Available The topic of this article is the literary discourse of Young Estonia; its relations with other important Estonian literary discourses from the beginning of the 20th century, and its relations with colonial cultural discourse. First, I give an overview of the basic positions of the Young Estonians’ literary discourse, the main shapers of which were Friedebert Tuglas, Gustav Suits, later on Johannes Semper as well. Next, I present the basic principles of three Estonian literary discourses from the beginning of the 20th century, which were also important to the Young Estonians: nationalist-naturalistic (close-to-life; socialist/ class-based, and 20th century modernist. The Young Estonians began as nationalists and/or socialists. During the formation of Young Estonia’s discourse at the end of the first and beginning of the second decade of the 20th century, 20th century modernism began, which the Young Estonians regarded first and foremost with irony. The Young Estonians’ literary discourse is a mixture of aestheticism, decadence, symbolism, romanticism, and classicism. The point of departure for the Young Estonian approach to literature was eurocentrism. They took a superior and negative view of existing Estonian literature, which they regarded as having fallen drastically behind Europe. Since it was unable to build on its own foundation, it had to borrow from Europe. The discourse’s understandings of cultural values – theory, reading, knowledge, ready-made culture, derive from the centrality of the dynamic of borrowing. New culture could be created freely, without the support of previous local tradition; it was to be an elite culture, while the writer remained an individualist. Literature was to follow the principle of art for art’s sake; aesthetics and the form of the work of art were basic criteria. The formal ideals of the work of art were classicist: unity, integrity (wholeness, harmony, order, logic, etc. Thirdly, I outline the basic positions of colonial thinking about culture, and compare the Young Estonian approaches to literature with colonial thought. The foundation of colonialism is eurocentrism: if Europe is the core; everything else belongs to the periphery, and regarded from the outset as of lesser inherent worth: colonialism is characterized by a superior and negative attitude toward local culture. The colonizing culture interprets its own values and experiences as universals; these are congruent with the outlines of what is deemed to be ”European” culture. The mission of colonialism is to bring the periphery into the sphere of influence of the enlightened core. Though colonial thinking does not commit itself to any specific artistic movement or style, its most important tenet with regard to evaluating art is classicist, emphasizing enduring values and good ”handiwork”. The bases for the creation of colonial culture are ready-made models and categories, and the outcome of colonization is culture constructed according to a model. The outcome of the analysis is that the Young Estonian way of thinking is in close consonance with the colonial way of thinking. Those attitudes that Young Estonians did not adopt, or those they relinquished and discarded, are in opposition to it. My own basic claim is that the Young Estonians unconsciously carried a colonial way of thinking, and that they might be considered to be self-colonizers.

  11. Efficacy of nitric oxide, with or without continuing antihypertensive treatment, for management of high blood pressure in acute stroke (ENOS)

    DEFF Research Database (Denmark)

    Bath, Philip M W; Woodhouse, Lisa; Scutt, Polly

    2015-01-01

    BACKGROUND: High blood pressure is associated with poor outcome after stroke. Whether blood pressure should be lowered early after stroke, and whether to continue or temporarily withdraw existing antihypertensive drugs, is not known. We assessed outcomes after stroke in patients given drugs......·91-1·13; p=0·83), and with continue versus stop antihypertensive drugs OR was 1·05 (0·90-1·22; p=0·55). INTERPRETATION: In patients with acute stroke and high blood pressure, transdermal glyceryl trinitrate lowered blood pressure and had acceptable safety but did not improve functional outcome. We show...

  12. Study about the effect of microgravity on biofunctions; Seitai kino eno bisho juryoku no eikyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    A study is made for the elucidation of the effect of microgravity on biofunctions. The protoplast of shiitake is exposed to microgravity and then cultured, and a significant difference occurs. The rate of colony formation by cell fusion in the test sector is found to be two times higher than that in the control sector. When swimming ciliates which are unicellular are suddenly exposed to microgravity, their swimming speeds changed differently according to the direction of swimming. When a mouse subjected to acupuncture for three days is exposed to microgravity, its water metabolism and excretory function are enhanced. A mouse treated with Chinese medicine reacts in the similar way. The change due to microgravity in the amount of acetylcholine in the hypothalamus is found characteristically time dependent. Mice infected with lethal herpes viruses just after exposure to microgravity die at a rate in proportion to the number of exposures and to the amount of viruses given. The migration speed of chemical stripes in case of a gel-base BZ (Belousov-Zhabotinsky) reaction under microgravity is equal to that on the ground. The trigger wave migration speed in case of a water solution-base BZ reaction under microgravity is reduced to approximately 80% of the speed on the ground. 12 refs., 31 figs., 3 tabs.

  13. Report to the Nuclear Regulatory Commission from the staff panel on the Commission's determination of an Extraordinary Nuclear Occurrence (ENO)

    International Nuclear Information System (INIS)

    1980-01-01

    The Panel finds that the first criterion, pertaining to whether the accident caused a discharge of radioactive material or levels of radiation offsite as defined in 10 CFR 140.84, has not been met. It further finds that there is presently insufficient information to support any definitive finding as to whether or not the second criterion, relating to damage to persons or property offsite as defined in 10 CFR 140.85, has been met. Since the Panel has not found that both criteria have been met, it recommends that the Commission determine that the accident at Three Mile Island did not constitute an extraordinary nuclear occurrence

  14. Optimum design of automobile seat using statistical design support system; Tokeiteki sekkei shien system no jidoshayo seat eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Kashiwamura, T [NHK Spring Co. Ltd., Yokohama (Japan); Shiratori, M; Yu, Q; Koda, I [Yokohama National University, Yokohama (Japan)

    1997-10-01

    The authors proposed a new practical optimum design method called statistical design support system, which consists of five steps: the effectivity analysis, reanalysis, evaluation of dispersion, the optimiza4ion and evaluation of structural reliability. In this study, the authors applied the present system to analyze and optimum design of an automobile seat frame subjected to crushing. This study should that the present method could be applied to the complex nonlinear problems such as large deformation, material nonlinearity as well as impact problem. It was shown that the optimum design of the seat frame has been solved easily using the present system. 6 refs., 5 figs., 5 tabs.

  15. Adaptation of topology optimization on truck-structure; Kinshitsukaho ni yoru iso saitekika shuho no truck kozo eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Tachibana, H; Kojima, A; Chiba, S [Mitsubishi Motors Corp., Tokyo (Japan)

    1997-10-01

    An optimization using the homogenization method has been applied to a truck-structure on the concept design stage. A truck-structure is grouped into 3 classes (thin plate structure , thick plate structure and solid structure), then example, effectiveness and method for the application for the purpose of weight reduction , high rigidity and high eigen-frequency are introduced. 3 refs., 24 figs., 1 tab.

  16. Role of iNOS and eNOS expression in a group of Egyptian diabetic and nondiabetic nephropathy patients

    Directory of Open Access Journals (Sweden)

    Mayssa I. Aly

    2013-01-01

    Conclusion The presence of iNOS is associated with tubular damage resulting in renal failure. The upregulation of NO in diabetes mellitus type 2 may explain the endothelial dysfunction that is associated with almost all diabetic complications.

  17. Latest trends in variable speed drive systems. ; Application to elevators. Kahensoku drive system no saishin doko. ; Elevator eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Inaba, H. (Hitachi, Ltd., Tokyo (Japan))

    1992-07-12

    The history of the elevator development may be summarized as a challenge to heights of buildings, pursuit of energy saving, and realizing comfort. Transfer of its drive system to the inverter drive system has been nearly completed keeping the pace with advance of electronic technologies. This paper describes control systems of elevators, effects of introducing inverters, and future trends. The inverter system used widely now for standard elevators with a speed lower than 105 m/min consists of a converter to rectify the utility power, a regenerative power consuming circuit to suppress overvoltage in the DC stage, and an inverter to supply power to induction motors. Those elevators exceeding a 120 m/min speed use power regenerating inverter system, with its converter controlled using micro computers according to loads and speeds of motors. The inverter system is particularly marked for having realized energy saving, lower power consumption, lower environmental pollution (from harmonics), comfort and maintainability at high levels. 13 refs., 9 figs.

  18. Kinnisvaraäri ajas Pärnu linnajuhid omavahel tülli / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Pärnu linnavolikogu esimees Ahti Kõo peab ebaeetiliseks linnapea Mart Viisitamme ja spordiseltsi Põhjakotkas juhatuse esimehe Ivar Hallopi vahel sõlmitud ühtse kavatsuse protokolli. Ilmunud ka Pärnu Postimees 17. nov. lk. 1

  19. Pärnu leivatööstusest võib saada sakslaste allhankija / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Pere Leib Tootmine plaanib kolida leiva-saia tootmise suuremas osas Tartusse ja keskenduda Pärnus eksporttoodete valmistamisele Saksa turule. Arvamust avaldab Pere Leib Tootmise juhatuse liige Eero Mikenberg

  20. Rapala landitehas püüdis tänavugi suurima kala / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Maailmakuulsa landitootja Rapala Pärnus Reldori tööstuskülas asuv tehas pääses teist aastat järjest Pärnumaa aasta ettevõtete edetabeli etteotsa. Diagramm: Rapala Eesti ASi majandustulemused. Vt. samas: Pärnumaa ettevõtete tipp 50

  1. Häädemeeste vallavanem jäi kihutamise pärast jalameheks / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Ilmunud ka: Postimehes 9. nov. lk. 5 lüh.Korduva kiiruseületamisega silma paistnud Häädemeeste vallavanem Urmas Aava peab viimase kihutamise eest kuuks sõiduõiguseta jätmist liiga karmiks karistuseks

  2. MiR-21 is induced in endothelial cells by shear stress and modulates apoptosis and eNOS activity

    International Nuclear Information System (INIS)

    Weber, Martina; Baker, Meredith B.; Moore, Jeffrey P.; Searles, Charles D.

    2010-01-01

    Mechanical forces associated with blood flow play an important role in regulating vascular signaling and gene expression in endothelial cells (ECs). MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. miRNAs are known to have an important role in modulating EC biology, but their expression and functions in cells subjected to shear stress conditions are unknown. We sought to determine the miRNA expression profile in human ECs subjected to unidirectional shear stress and define the role of miR-21 in shear stress-induced changes in EC function. TLDA array and qRT-PCR analysis performed on HUVECs exposed to prolonged unidirectional shear stress (USS, 24 h, 15 dynes/cm 2 ) identified 13 miRNAs whose expression was significantly upregulated (p · ) production. These data demonstrate that shear stress forces regulate the expression of miRNAs in ECs, and that miR-21 influences endothelial biology by decreasing apoptosis and activating the NO · pathway. These studies advance our understanding of the mechanisms by which shear stress forces modulate vascular homeostasis.

  3. Introduction of electric double layer capacitors in the solar-EV; Solar denki jidosha eno denki nijuso condenser no oyo

    Energy Technology Data Exchange (ETDEWEB)

    Fujinaka, M [Tokyo Denki University, Tokyo (Japan)

    1996-10-27

    A basic experiment was carried out on a supplementary power supply, in which solar cells and electric double layer capacitors(EDLC) were combined for a DC/DC converter, for the use of a solar-electric vehicle (S-EV); and in actuality, an S-EV was loaded with the power supply with a running test conducted on a public road. The EDLC was found effective and useful for avoiding temporary lowering of voltage and momentary break. An electric supply was thereby made possible for an emergency light without the use of the DC/DC converter. However, in a tunnel or a night driving and in case of failure of the DC/DC converter, an operating time of only 7 minutes or so was affordable with the EDLC having a capacity of 100F. Moreover, particularly with a heavy load, it was impossible to maintain a voltage for many hours. Under the circumstances, an S-EV design would primarily require two sets of independent DC/DC converter loaded in the future. The EDLC, young after it was developed, still has a small energy density compared with a lead storage battery. Yet, an EDLC with a higher performance being developed, there is a possibility that it will be applied to S-EV`s by utilizing its characteristics such as a high efficiency and a long service life. 4 refs., 8 figs.

  4. Estimation of the charge quantity from solar cell to battery; Taiyo denchi ni yoru chikudenchi eno juden yosoku

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, K; Nishitani, M [Daiichi University, College of Technology, Kagoshima (Japan)

    1996-10-27

    In performing an experiment of running a small electric vehicle by installing solar cells in it and by charging the storage battery at a specific voltage, an estimation was made on the charge quantity to the battery for each solar altitude and inclination of a module at different hours. The solar altitude was determined at Daiichi University, College of Technology, according to the month and the time of day from a formula using day-declination and time equation of a chronological table of science. The quantity of global solar radiation was determined by resolving the solar radiation into its direct and diffuse components on the basis of the extra-terrestrial solar radiation quantity with the change in radius vector taken into consideration; and then, the global solar radiation on the inclined face was obtained from the angle of inclination and incidence. On the roof of a Daiichi University building, solar cell modules were installed facing north and south at 0{degree}, 30{degree}, 45{degree}, 60{degree} and 90{degree} each, so that a short-circuit current was measured for each differently inclined angle. As a result of the experiment, shown in an regression formula is a relation between the temperature conversion value of the maximum output of the solar cell at the standard temperature of 25{degree}C and the quantity of solar radiation on the inclined surface. Consequently, it enabled the prediction of a charging quantity, in the case of running a small vehicle with solar cells installed, from the quantity of solar radiation on the inclined surface in the clear weather. 2 refs., 4 figs., 2 tabs.

  5. Problems in green improvement projects in recent city planning. Kinnen no toshi keikaku ni okeru ryokuchi keikaku eno kadai

    Energy Technology Data Exchange (ETDEWEB)

    Funabiki, T. (Ministry of Construction, Tokyo (Japan))

    1992-08-06

    The present city plans are questioned on the formation of a good city environment, improvement of mansion/building supply, due to the new city problems on the Tokyo concentration and re-increase of the land value recently. Based on the discussion of the central city planning council, various improvement of the system were made by government. As a structure plan of the city, the green area plan that the balance between the urban land use and the national land use is distributed, must be included. As the problem of the green area plan on the system of city plan, this paper explained the distinguish between city area and city adjustment area, the green master plan about the green area preservation in long time, the green area system corresponded to the policy for realizing the plan of preserving the green area mainly, and the park work and green area policy corresponded to the policy for realizing the plan of creating the green area.

  6. President tunnustab Eesti riigi edendajate elutööd / Silja Joon, Ester Vilgats, Eno-Gerrit Link

    Index Scriptorium Estoniae

    Joon, Silja, 1966-

    2008-01-01

    Pärnumaalased, kes pälvivad iseseisvuspäeva puhul ja Eesti riigile osutatud teenete tunnustamiseks president Toomas Hendrik Ilveselt riiklikud autasud. Juuresolevatel fotodel Valgetähe V klassi ordeni pälvinud koorijuht ja muusikaõpetaja Toomas Voll ja Kotkaristi IV klassi teenetemärgi pälvinud Lääne prefektuuri politseiametnik Kalvi Almosen. Vt. ka lk. 1: Enn Hallik. Tatjana Jaanson üllatus suurest aust. Sõudetreener pälvib Valgetähe IV klassi teenetemärgi

  7. Application of laser cladding to the aeroengine component. Koku engine buhin eno laser nikumori yosetsu no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Morita, A [Ishikawajima-Harima Heavy Industries, Co. Ltd., Tokyo (Japan)

    1991-08-01

    Keeping the pace with recent development and application of laser cladding, hard-facing is used more frequently on turbine blades made of superalloys used in aeroengines. This paper explains the basic principles and features of laser hard-facing technique, welding parameters, and examples of practical use. Examples of practical use include application to turbine blades used in ALF502R-5 turbo fan engines for commuter aircraft and high-pressure turbine blades used in RB211 turbo fan engines for large passenger aircraft. In the former engine, improvement of abrasion resistance was intended at the shroud section where blades are in contact with each other, for which inconel was used as the base material and CO-group alloy as the welding material. The welding used a powder supply system with a laser generator oscillating CO{sub 2} at 5 kW and employing a beam collecting mirror plus scanner to attain a beam covering wider width. Faces with higher performance were obtained than by the conventional TIG welding, and the finishing time was decreased largely. 2 refs., 9 figs., 3 tabs.

  8. Crankshaft strength and rigidity analysis and application to shape optimization; Crank jiku kyodo gosei kaisekiho to keijo saitekika eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Numajiri, S [Mitsubishi Motor Corp., Tokyo (Japan); Tamura, Y [Mitsubishi Automotive Engineering Co. Ltd., Tokyo (Japan)

    1997-10-01

    A crankshaft bending stress analysis method using an elastically supported continuous beam model has been established based on the exact evaluation of the stress concentration at fillet R sections and the crankshaft rigidity. Through various examinations, it was revealed that the calculation results of the bending stress well agreed with the actual measurements. This allowed the reliability analysis and the rigidity analysis to be used to determine optimized crankshaft specifications and web shape, which in turn made it possible to apply this method to the weight reduction of a crankshaft (material removal from web). 1 ref., 7 figs., 1 tab.

  9. Power feeding to terminal devices in optical subscriber network; Hikari fuaiba tsushin ni okeru tanmatsu kiki eno kyuden

    Energy Technology Data Exchange (ETDEWEB)

    Yamasaki, M

    1998-09-01

    This paper outlines present state of the feeding system in the light access system in consideration of backup in case of home power failure. In the optical communication, equipment with bilateral conversion function for light and electricity has to be installed both on communication service side and home side. Outline of the light access system and equipment requiring backup are shown. The following items are explained: main types of the light access system, such as FTTC (fiber to the curb), FTTP (fiber to the pole), FTTH (fiber to the home) and HFC (hybrid fiber coax), and the feeding system; problems on feeding system for the light access system. Key elements of ONU (optical network unit) for FTTH and construction of the ONU power source are illustrated. The ONU power source is composed of a backup power supply unit and main body supply unit. Backup power is indispensable to the ONU and analog telephones: output is supplied by the backup batteries in power failure. For miniaturization of the backup power source, power supply circuits and backup batteries have to be miniaturized simultaneously. History of miniaturization is explained on the experimental models. 6 refs., 7 figs., 1 tab.

  10. Role of civil technology towards environmental conservation and creation; Kankyo hozen/sozo eno doboku gijutsu no yakuwari

    Energy Technology Data Exchange (ETDEWEB)

    Kato, T. [Maeda Corp., Tokyo (Japan)

    1995-01-15

    The recent circumstances domestic and abroads around `environment` are showing a kaleidoscopic movement with the Earth Summit in 1993 as a turning point, such as the materialization of the Environmental Fundamental Law, enactment of environmental policy fundamental principle by the Ministry of Construction, and furthermore and adoption of the earth environmental action plan-agenda 21-by the Japan Society of Civil Engineers. Under the circumstances like this the civil work execution study committee has planned this panel discussion for study, for a purpose of catching hold of the environmental problem from a standpoint of the actual constructor and furthermore searching for what should be done. As a consequence, the various opinions have been presented as follows: Namely, in order to grapple with the environmental problems it is required for that a co-operation work of the civil engineers should be done with the people in the other field. As for the environmental problems, a discussion forecasted for the future is necessary. Because an environment itself is fuzzy matter, it would be better not to give the too clear solution for a concept. An attitude to make much of the matter which can not be counted is needed. The mitigation (influence alleviation countermeasure) is required for applying flexibly.

  11. Realization of an accelerator for environmental refreshment in case of soil; Fukyugata kasokuki ni yoru kankyo sosei eno koka

    Energy Technology Data Exchange (ETDEWEB)

    Isogai, H [Electrotechnical Laboratory, Tsukuba (Japan)

    1997-11-25

    Learning from a history of the earth of ancient times, the paper studies effects of refreshing environment and soil or making them natural and harmless mostly using primitive bacteria, and develops element technology which is industrially accelerating. The spread type accelerator working only by solar energy was installed and operated for experiment. As indices of refreshment effects, a study was comparatively made between a sugar degree of sweet potato and a yield shown in the number of potatotimesunit weight. An EM propagation garbage layer is formed, and the two sections were installed of a site of the actual installation of the spread type accelerator and a site naturally left alone. Other effects of weather, temperature and humidity which are external factors and general fertilization are offset as common modes, and a consideration was so made that only refreshment effects of primitive bacteria can be relatively compared. Acceleration effects in using accelerator for growing sweet potato could be quantitatively confirmed. As to sugar degree, because the last study was made in the beginning of summer, the underground temperature was high enough. Even only EM effects were fully effective. This time, the weather was much colder, and it was found to be a must to use accelerator. 2 figs.

  12. Port Artur 3 arhitektuurikonkursi võidutöö sündis Itaalias / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Pärnu kesklinna jõeäärse ala rahvusvahelise arhitektuurikonkursi tulemustest. Võitis itaalia arhitektide projekt "Flood". Teise koha võitis projekt "Istu ja vaata" (Jan Skolimovski ja Peeter Loo), kolmanda koha "Nicesaar" (Ott Kadarik, Villem Tomiste, Mihkel Tüür)

  13. Approach to load leveling in Kansai Electric Power Co. Inc.; Kansai Denryoku no fuka heijunka eno torikumi

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-09-01

    This paper presents an electric hot-water heater and ice storage air conditioning system as systems to be recommended for load leveling. Electric hot-water heater is featured by safety, cleanliness, silence and convenience because of no use of fire. Its electricity charge is only 7.15 yen/kWh less than 1/3 of that for ordinary homes because of use of midnight power. Mainly used MPU-control type electric hot-water heater is more economical because of a 15% discount system. Ice storage air conditioning system is operated in the daytime using ice made by midnight power. It is featured by reduction of facility and installation costs due to the small capacity of heat source equipment, use of inexpensive midnight power, and reduction of running cost due to small contract demand. However, since an ice storage air conditioning system is in the initial stage of diffusion, its initial cost is expensive as compared with conventional non-heat storage air conditioning systems, remaining the issue of cost reduction. 3 figs., 1 tab.

  14. Load levelling measures being tackled by shikoku Electric Power Co. Inc.; Shikoku Denryoku Kabushi Kaisha no fuka heijunka eno torikumi

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-11-01

    For the electric power companies, the activity of pushing forward the load levelling contributes greatly to the curtailment of cost by means of effective formation and utilization of facilities. It is planned to take up more positive activities with a target of 3% improvement in the load factor in ten years. Concrete measures for further promotion of load levelling are the diversity of the rate menu of appraising the load levelling effort of the customers and the promotion of the spread of equipment and systems relating to load levelling such as electric water heating apparatuses and thermal energy storage air conditioning systems. The rate system has been reviewed taking the opportunity of rate revision made in January, 1996. Particularly, further load shift to midnight time zone is expected as a result of better rate incentive for customers which is brought about by large reduction of the rate level due to the cost reduction effect accompanying the improvement in atomic power generation ratio. Outlines are given on the fields relating to general households, business, dwellings, buildings, and industry. 3 figs., 1 tab.

  15. Approach undertaken by our Company for leveling electric loads; Chubu Denryoku Kabushi Kaisha no fuka heijunka eno torikumi ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    Measures for leveling the electric loads relying upon two facets - tariff system and business activities - in Chubu Electric Power Co., Inc. are presented. Firstly, the existing pricing systems for midnight electric power and electric light according to the period of time for the household as well as those of seasonal and time zone electric power, modifiable contract with the time zone and so forth for large industry users, together with a similar contract for the owner of regenerating installation are outlined. In addition, a price reduction system is indicated in favor of a scheduled shutdown of large industry operations as a means of avoiding electric consumption during weekdays in summer and between 13h and 16h when large demands exist. Further, a selective contract system put in service in 1995 and the price reduction for ice regenerating air conditioning system are cited. As for business facet, 1995 is regarded as the first year of the ice regenerator for its generalization through exhibition and promotive campaign to the foods processing industry, and the application of the latent heat regenerative system to perishable foods is recommended. Moreover, installation of energy saving type automatic vending machine, so-called eco-vendor is encouraged for its merit of peak-cut and energy price lowering. Lastly, activities to extend the use of electric calorifiers and the regenerative floor heating are mentioned. 2 figs., 1 tab.

  16. Load leveling efforts of The Hokkaido Electric Power Co. Inc.; Hokkaido Denryoku no fuka heijunka eno torikumi ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    The Hokkaido Electric Power Co., Inc., aiming to enhance power generation efficiency through power load levelling, strives to expand and substantiate its electricity billing menu and to popularize and encourage the use of levelling-oriented apparatuses and systems most of which are designed for utilizing midnight power. The billing menu has in it a snow-melting power which is cut off for load levelling during the peak demand time zone. For domestic use, a time zone-specified lighting system named Dream Eight is created, which is one of the billing systems dependent upon time zone. Introduced therein for industrial use is a demand/supply adjustment contract system. Furthermore, in compliance with the amended Electricity Business Law that came into force in 1995, efforts are under way for revising the period wherein power is to be supplied for melting snow, expanding the scope of application of the power supply system dependent upon time zone, and newly introducing a heat accumulation assisted peak adjustment contract system and an operation adjustment contract system. As for business efforts in relation to load levelling, the company proposes household electrical systems centering about 200V high-efficiency apparatuses, electric water warmer contributing to the enhancement of year-round load levelling, popularization and reinforcement of electric snow melting systems, and power utilizing technologies capable of meeting local demands raised for example by agriculture and fishery.

  17. Investigation of load leveling in Hokuriku Electric Power Co., Inc.; Hokuriku denryoku no fuka heijunka eno torikumi

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    Hokuriku Electric Power Co., Inc. aims at an around 2% improvement of the load factor up to 2005, by which the quick and proper service and the proposal of load leveling menu are planned. This paper describes an outline of the investigation of load leveling. Various programs have been proposed so that the customers can further shift the load by their consideration. Proposed systems include the time-of-day electricity rate system, the load regulation contract system for industries, the seasonal time-of-day rate system, the electric power system for snowmelt in which the load is dumped at the peak, and the secondary electric power system for snowmelt. Accompanying with the revision of electric utility law, the enlargement of its available time, the price reduction, and the discount rate system for the ice regenerative air conditioners have been provided. For the business activities, a demonstration model house was exhibited to indicate a proper house with local characteristics in Hokuriku district. Furthermore, the spreading activities of regenerative systems and the consulting activities have been positively promoted. 4 figs., 1 tab.

  18. Efforts of load leveling in Tohoku Electric Power Co. Inc.; Tohoku denryoku no fuka heijunka eno torikumi ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    This paper describes efforts of load leveling in Tohoku Electric Power Co., Inc. from the viewpoint of the rate system and business development. For the approach from the rate system, various rate menus for the peak shift have been arranged for domestic and large industry customers. To create the midnight demand, spread and expansion of electric hot water service are promoted. Consequently, the contract of midnight power has reached 914,000 kW at the end of 1995, which was 774,000 kW in 1985. To spread the ice regenerative air conditioning systems, the grant of incentive to the manufacturers and special discount of electricity rate have been conducted. These systems have been introduced in the eight places of Tohoku Electric Power`s business. The spread incident system has been created to introduce the automatic vending machines for beverages with the power peak cut function. Unused energy, such as exhaust heat from the non-treated sewage water and substations has been utilized as a heat supply project for city redevelopment. Multi-functional heat pump with regenerative function has been developed. This system aims at the peak shift by combining multi-functional heat pump and electric hot water service. 2 figs., 2 tabs.

  19. Ubiad1 is an antioxidant enzyme that regulates eNOS activity by CoQ10 synthesis

    NARCIS (Netherlands)

    Mugoni, V.; Postel, R.; Catanzaro, V.; De Luca, E.; Turco, E.; Digilio, G.; Silengo, L.; Murphy, M.P.; Medana, C.; Stainier, D.Y.; Bakkers, J.; Santoro, M.M.

    2013-01-01

    Protection against oxidative damage caused by excessive reactive oxygen species (ROS) by an antioxidant network is essential for the health of tissues, especially in the cardiovascular system. Here, we identified a gene with important antioxidant features by analyzing a null allele of zebrafish

  20. Application of CO2 laser cutting machine to shipbuilding; Zosen no setsudan eno CO2 laser no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Fujimoto, Y. [Mitsui Engineering and Shipbuilding Co. Ltd., Tokyo (Japan)

    1998-11-01

    Carbon dioxide laser has been applied to cutting works in shipbuilding. As NC cutter was obsolete, CO2 laser cutting machine with a rated output 3 kW was introduced. According to the specifications of the machinable plate, the maximum thickness is 19 mm, and effective width and length are 5.4 m and 29.1 m, respectively. As the cutting width is wide and the running distance of laser beam is long, the error of light axis is expanded only by a small error of irradiation angle, which results in the inconvenience. Stable operation was realized by improving the fixation of reflecting mirror. Right angle cutting section with a cutting curve below 0.5 mm was obtained, and cutting time was reduced by the one-line cutting by which both sections can be available with a single cutting line. For the cutting of thin plate with a thickness less than 10 mm, strain was not formed, and remedy was not required. Block strain was remarkably reduced during the assembly process. Maintenance-free operation without monitoring can be performed for a long time. This machine is operated at night and lunch time, resulting in the reduced processes. The working environment is appropriate without noise and dust. Facility cost and maintenance cost were also reduced. Lower cutting speed is a weak point, when compared with the plasma cutting machine. The present machine is not applied to aluminum plates with high surface reflectivity. 13 figs.

  1. enos a ochrana informací v mobilních telekomunikacích

    OpenAIRE

    Pacák, Pavel

    2011-01-01

    Charles University in Prague Faculty of Arts Institute of Information Studies and Librarianship Study program: Information Studies and Librarianship Field of study: Information Studies and Librarianship Pavel Pacák The transfer and protection of information in mobile telecommunications Bachelor thesis abstract The work focuses on the transfer and protection of information in mobile telecommunications. First, historical development of each technology used is introduced. Also different forms of...

  2. Ecological processes for Environmental Impact Assessment in Coastal Waters; Engan no kankyo eikyo hyoka (EIA) eno seitaigakuteki apurochi

    Energy Technology Data Exchange (ETDEWEB)

    Ishikawa, Kimitoshi. [Environmental Assessment Dept., Tokyo (Japan)

    1999-03-25

    This paper focuses on a method for estimating adverse effects on natural environment by economic development in coastal waters in Japan. In particular, the most critical relations concerning the impact on marine organisms is pointed out and discussed according to an environmental impact assessment. Relations between environmental impact assessment and marine organisms needs a quantitative ecological approach in order to succeed in sustainable development of coastal waters management. Recently, the Environment Agency of Japan pointed out the unreliability and the theme on accuracy and the staff for biology and ecology in the environmental impact study. Ecological response to environmental impact occurs in a wide spectrum of physiological and biological and biological functions. Therefore biological monitoring measures must correspond to the given time-space scale of natural mechanisms. For the evaluation of environmental impact, it is desire able to develop of experimental technics and collect biological and ecological basic data for the object. I Reconstructing the following: 1. Reconstructing a clearlistic EIA process by EIA agencies and practitioners, 2. Scorping the biological and ecological issues by EIA professionals, 3. Using the current computer technology, 4. Considering monitoring systems over scales of time and space for the ecological target and 5. Making available to public all monitoring data and reports of EIA. (author)

  3. Application fo tunneling and atomic force detection to machines and scientific instruments. Tunnel gensho ya genshikan no mechatronics eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Kawakatsu, H. (The University of Tokyo, Tokyo (Japan). Institute of Industrial Science)

    1991-11-01

    It is now about ten years since the scanning tunnel microscope (STM) and the atomic force microscope (AFM) were invented. These microscopes are used very importantly today as a surface analyzer capable of atomic order analysis for their very high resolution. The STM and AFM obtain images of atomic orders by means of mechanical scanning of the probes. This means that the microscopes can control positioning, force detection and displacement in regions of sub-nanometer orders. Examples of researches may include detection of acceleration, length measurement, positioning, force detection, nano-tripology, processing and gravitational wave detection. As described above, this paper notes the researches derived from the operational principles of the STM and AFM, rather than the researches on the observation objects. The great diversity of the researches taken up and the greatness in the number of researches in the STM and AFM gather how superbly germinative these were. 34 refs.

  4. Astronaut training plans and training facilities in Japan; Uchu hikoshi tanjo eno michi (kunren to kunren setsubi)

    Energy Technology Data Exchange (ETDEWEB)

    Harada, C. [National Space Development Agency of Japan, Tokyo (Japan)

    1999-10-05

    Introduced are the training of astronauts for duties aboard a space shuttle, training provided by NASDA (National Space Development Agency of Japan), and training facilities. The astronaut candidate training course involves space science, space medicine, ocean science, and others, in addition to flight training aboard the T-38 jet trainer, emergency procedure training, shuttle system training, weightlessness training aboard the KC-135 jet plane on a ballistic flight, and SCUBA training. After candidates are named to serve aboard the space shuttle, they are to undergo training related to the shuttle system, emergency exit, adaptation to the surroundings, and the space laboratory system. As for ISS (international space station), astronauts will have to construct the station, and to stay there for a long time operating and maintaining the station and manipulating various experimental apparatuses. The astronaut training process in Japan covers approximately four years, including candidate training, advanced training, and mission dependent training. The training facilities include a weightless environment test system, low-pressure environment adaptation training system, etc., available at NASDA's Tsukuba Space Center. (NEDO)

  5. Ilves viskas koos Bedwettersiga näppu. President Ilves kohtus õpilaste ja kaitseliitlastega / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Münchenis MTV Euroopa muusikaauhindade jagamisel Uue Euroopa Heli kategoorias lauluga "Dramatic Letter to Conscience"esikoha võitnud Pärnu pop-punkbändist Bedwetters ja president Toomas Hendrik Ilvese Pärnu Sütevaka humanitaargümnaasiumi ja Kaitseliidu Pärnumaa maleva külastusest 9. novembril

  6. Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Fujita, M; Ike, M [Osaka University, Osaka (Japan). Faculty of Engineering

    1993-11-10

    This paper summarizes the following techniques: a gene engineering method for bioremediation and wastewater treatment, microorganism breeding using the former method, and a monitoring technique for genetical and ecological stability of genetically engineered microorganisms. Recombination bacteria reinforced with PH genes showed higher phenol removing rate than wild strains, but presented accumulation of catechol in such a large quantity as cannot be seen in wild strains, with the complete degradation rate rather decreased. Gene recombined bacteria structured by introducing the recombined plasmid, pBH500, had high genetic stability when P.putida BH-1 is used as a host. E.coli C600 having recombined plasmid and P.putida BH were added and cultivated in activated sludge. As a result, both recombined bacteria showed rapid logarithmic decrease just after the addition, then, maintained the relatively stable population groups, and remained in the activated sludge for an extended period of time. In monitoring techniques, the colony hybridization process detected clearly the gene recombined bacteria. 9 refs., 7 figs., 1 tab.

  7. Report to the Nuclear Regulatory Commission from the staff panel on the Commission's determination of an Extraordinary Nuclear Occurrence (ENO)

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-01-01

    The Panel finds that the first criterion, pertaining to whether the accident caused a discharge of radioactive material or levels of radiation offsite as defined in 10 CFR 140.84, has not been met. It further finds that there is presently insufficient information to support any definitive finding as to whether or not the second criterion, relating to damage to persons or property offsite as defined in 10 CFR 140.85, has been met. Since the Panel has not found that both criteria have been met, it recommends that the Commission determine that the accident at Three Mile Island did not constitute an extraordinary nuclear occurrence.

  8. Design and erection of the MUSEUM BRIDGE; Bijutsu no sekai eno yume no kakehashi. MUSEUM BRIDGE no sekkei seko

    Energy Technology Data Exchange (ETDEWEB)

    Ishihara, S.; Tsunekawa, M.; Umda, A.; Kishimoto, Y.; Yotsukura, Y.; Miitsu, K.; Okada, S.; Furukawa, M. [Kawasaki Heavy Industries, Ltd., Kobe (Japan)

    1998-12-20

    MUSEUM Projects for the religious corporation of Shinji-Shumeikai, at Shigaraki, Shiga prefecture. Fundamental design was by a project team led by the world-famous architect I.M. Pei. The museum is located in a prefectural park surrounded by forests. The design concept of the bridge aimed to connect Shangri-La (museum) to the real world. In order to satisfy this purpose, a cable-stayed bridge with external tendon was adopted as an original structure style, and details such as handrails, gratings, and gloss painting taken into consideration. The authors report the characteristics of the bridge and the sequence of erection. (author)

  9. ORF Alignment: NC_003295 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available TEIN ... [Ralstonia solanacearum GMI1000] ... Length = 101 ... Query: 1 ... MIPGELLPQDGDLELNAGRPTLTVTVANT...GDRPVQIGSHYHFHEVNDALRFDREATRGYR 60 ... MIPGELLPQDGDLELNAGRPTLTVTVANT...GDRPVQIGSHYHFHEVNDALRFDREATRGYR Sbjct: 1 ... MIPGELLPQDGDLELNAGRPTLTVTVANTGDRPVQIGSHYHFHEVNDALRFDREATRGYR 60 ...

  10. ORF Alignment: NC_005072 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein S8 ... [Prochlorococcus marinus subsp. pastoris str. CCMP1986] ... Length = 128 ... Query: 5 ... DPISDMLTRIRNASQKKHTST...AIPASKMSLSIAKVLQKEGFITDINEEGEGYKSQIVLGL 64 ... DPISDMLTRIRNASQKKHTST...AIPASKMSLSIAKVLQKEGFITDINEEGEGYKSQIVLGL Sbjct: 1 ... DPISDMLTRIRNASQKKHTSTAIPASKMSLSIAKVLQKE

  11. ORF Alignment: NC_002935 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein ... [Corynebacterium diphtheriae] ... Length = 153 ... Query: 139 PAIPDDGPEIIVMTHPLDNVSEIDDV...HAEFLDQPDDDWLELYHFRGKALPRHALELLRTT 198 ... PAIPDDGPEIIVMTHPLDNVSEIDDVHAEFL...DQPDDDWLELYHFRGKALPRHALELLRTT Sbjct: 1 ... PAIPDDGPEIIVMTHPLDNVSEIDDVHAEFLDQPDDDWLELYHFRGKALPRHALELLRTT 60 ... Qu

  12. ORF Alignment: NC_002939 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available GESSFNPLAINYNSNGTYDYGLMQINSSWEPALRK 79 ... ASAFCFEEAGNRYGISPQLLYAISKGESSFNPLAINYNSNGTYDYGLMQINSS...WEPALRK Sbjct: 1 ... ASAFCFEEAGNRYGISPQLLYAISKGESSFNPLAINYNSNGTYDYGLMQINSSWEPALRK 60 ... ...tein ... [Geobacter sulfurreducens PCA] ... Length = 101 ... Query: 20 ... ASAFCFEEAGNRYGISPQLLYAISK

  13. ORF Alignment: NC_003919 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein ... B [Xanthomonas axonopodis pv. citri str. 306] ... Length = 101 ... Query: 9 ... TSHRPYLLRALVEWIND...NGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD 68 ... TSHRPYLLRALVEWIND...NGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD Sbjct: 1 ... TSHRPYLLRALVEWINDNGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD 60 ...

  14. ORF Alignment: NC_006834 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein ... B [Xanthomonas axonopodis pv. citri str. 306] ... Length = 101 ... Query: 9 ... TSHRPYLLRALVEWIND...NGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD 68 ... TSHRPYLLRALVEWIND...NGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD Sbjct: 1 ... TSHRPYLLRALVEWINDNGMTPHVLVDAGLPGVQVPASAVKDGRVVLNIAERAVVGLQVD 60 ...

  15. ORF Alignment: NC_002939 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein-alanine acetyltransferase [Geobacter ... sulfurreducens PCA] ... Length = 146 ... Query: 1 ... MNTADCP...TIMPMTEGDLDEVLQIESDSFPRPWTRDHFVAELASPRSFPVVARSPGGLIVG 60 ... MNTADCP...TIMPMTEGDLDEVLQIESDSFPRPWTRDHFVAELASPRSFPVVARSPGGLIVG Sbjct: 1 ... MNTADCPTIMPMTEGDLDEVLQIESDSFPRPWTRDHFVAEL

  16. Fiscal 1999 survey report. Survey of leading countries' approaches to biomass energy development; 1999 nendo shuyokoku ni okeru biomass energy kaihatsu eno torikumi ni kansuru chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    The survey aims to help Japan deliberate its future biomass energy development strategy including the course Japan is to follow in its research and development of biomass energy by clarifying leading countries' approaches to the subject matter and trends of their research and development efforts in this connection. The states of biomass energy development in the U.S. and Europe are reported. In the U.S., President Clinton issued Executive Order 13134 on August 12, 1999, regarding bio-based products and bioenergy development. In this country, bioenergy and bio-based production technologies have developed to reach a stage where business pays, and commercial plants are in service. The U.S. Administration mentions as a strategy the efficient development of the bioenergy industry. In Europe, where resources are versatile and local, it is difficult to assess the economy of scale, and small-scale development efforts are being accumulated. Practical technologies under development mostly involve direct combustion. European measures are similar to U.S. measures in that such political goals as local development and employment promotion are firmly woven into them. (NEDO)

  17. Expression of iNOS, eNOS, and peroxynitrite-modified proteins in experimental anti-myeloperoxidase associated crescentic glomerulonephritis

    NARCIS (Netherlands)

    Heeringa, P; van Goor, H; Moshage, H; Klok, PA; Huitema, MG; de Jager, A; Schep, AJ; Kallenberg, CGM

    Nitric oxide radicals are recognized as important mediators in various physiological and pathophysiological processes. During inflammation, increased amounts of nitric oxide (NO) are produced, but it is unclear whether NO radicals are either protective or harmful. To obtain more insight into the

  18. Study on the effect of micro-gravity on bio-functions; Seitai kino eno bisho juryoku no eikyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The effect of micro-gravity on bio-functions and vital reaction was studied using the facility of Underground Gravity-free Experiment Center Co. On the effect on osteoblast shape and gene expression, although fluorochrome reacting with Ca was well taken into cells, no significant difference in Ca content in cells was observed before/after falling. Expression of genes related to cell propagation was controlled under micro-gravity. Protoplast fusion of Lentinus was unaffected by micro-gravity. The mRNA fragments of gravity sensitive mutant of rice plant were affected by micro-gravity. Paramecium was set swimming in solutions with different specific gravities. The reaction behavior of Paramecium was affected by the difference in specific gravity between cell bodies and solutions. The water content metabolism functions of a mouse with needle stimulus, in particular excretory, were slightly promoted by micro- gravity. The cortisol level in blood of a falling mouse group rose showing strong stress. As the preliminary study on the geotaxis of insects, motion of bagworm was observed. 12 refs., 38 figs., 4 tabs.

  19. Application to the field of medical and welfare with fluid power; Fluid power servo no iryo fukushi bun`ya eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayakawa, Y. [Nara Technical Coll., Nara (Japan)

    1999-05-15

    Reported herein are medical and welfare apparatuses activated by fluid power servos. Reference is also made to equipment now undergoing research and development. Numerous fluid power-driven welfare and caring apparatuses have been developed, some of which are named below. In an externally powered prosthetic leg, a rocking type hydraulic servo actuator is attached on the knee section. In this device, switching is performed between an active operation in which much torque is required for instance going up and down a stairway and a passive operation in which less torque is required for instance walking on a flat place, this for increased energy efficiency. In an externally powered orthosisis, an exoskeleton is installed on the lower extremities of a patient suffering from the paralysis of both legs, and enables physical exercises for rehabilitation. Such devices are controlled by one of the two methods, the master-slave method or the manual method. Devices for the transfer of patients include an in-home bathing assist system, powered assist suit for caring, powered assist chair, movable lift for transfer, pneumatic Rubbertuator-driven rehabilitation equipment, walk training device, and wavy motion pneumatic vibrator. (NEDO)

  20. Economical effect of introducing a photovoltaic system on future electric power system; Shorai no denryoku keito eno taiyoko hatsuden system donyu ni yoru keizaiteki eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Akata, N.; Kenmoku, Y.; Sakakibara, T. [Toyohashi University of Technology, Aichi (Japan); Kawamoto, T. [Shizuoka University, Shizuoka (Japan); Nakagawa, S. [Maizuru National College of Technology, Kyoto (Japan)

    1997-11-25

    Economic impact of introduction in the future of photovoltaic power generation (PV) systems into the commercial power system is discussed from the electric utilities` point of view. In the discussion, future demand for power is predicted from the demand for power in the base year, and the optimum power source configuration corresponding to the future demand and power generation curve are worked out by use of linear programming. Then, the fixed cost and variable cost are calculated for each of the power source components, and then the expected reduction in cost per unit of power generated is calculated. As the power sources, the hydroelectric, pumped storage, oil-fired, coal-fired, LNG-fired, combined cycle, and atomic technologies are considered. The conclusion is stated below. Upon introduction of PV power equivalent to 10% of the maximum demand power, the maximum power in the service area of Chubu Electric Power Co., Inc., will be reduced by 1.23GW in 2025, and the yearly demand power by 5472GWh. Since PV lowers the peak, an increased number of power sources low in variable cost will be used although such will be low in tracking capability. An increase in power demand will enhance the cost reducing effect of the PV system. 5 refs., 4 figs., 3 tabs.

  1. Conversion of char nitrogen to N2 under incomplete combustion conditions; Fukanzen nensho jokenka ni okeru char chuchisso no N2 eno tenka

    Energy Technology Data Exchange (ETDEWEB)

    Zhuang, Q.; Yamauchi, A.; Oshima, Y.; Wu, Z.; Otsuka, Y. [Tohoku University, Sendai (Japan). Institute for Chemical Reaction Science

    1996-10-28

    The effect of combustion conditions on conversion of char nitrogen to N2 was studied in the combustion experiment of char obtained by pyrolysis of coal. Char specimen was prepared by holding ZN coal of Chinese lignite in Ar atmosphere at 1123K for one hour. A batch scale quartz-made fluidized bed reactor was used for combustion experiment. After the specimen was fluidized in reaction gas, it was rapidly heated to start combustion reaction. CO, CO2 and N2 in produced gases were online measured by gas chromatography (GC). As the experimental result, under the incomplete combustion condition where a large amount of CO was produced by consuming almost all of O2, no NOx and N2O produced from char were found, and almost all of N-containing gas was N2. At the final stage of combustion, pyridinic-N disappeared completely, and pyrrolic-N decreased, while O-containing nitrogen complexes became a main component. It was thus suggested that O-containing nitrogen complexes are playing the role of intermediate product in combustion reaction. 7 refs., 4 figs., 1 tab.

  2. Application of photo-excitation reaction on titanium oxide thin film for control of wettability; Sanka chitann hakumakujo no hikari reiki hanno no nuresei seigyo eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, T.; Nakajima, A.; Hashimoto, K. [The Univ. of Tokyo, Tokyo (Japan); Takada, Y. [Kyushu Univ., Fukuoka (Japan)

    2000-03-31

    It is clarified that the photo-excitation hydrophilic reaction increasing wettability remarkably is induced by changing surface structure of titanium oxide radiated light. There are already many examples being in practical use of coating products applied hydrophilic reaction of titanium oxide surface such as drip-proof side millers for automobiles, self-cleaning building materials, and the like. When surface of titanium oxide having high activities for oxidisation and decomposition is coated organic materials and radiated light, wettability of surface changes as organic materials are decomposed. If it is possible to change wettability shaping pattern drastically by radiating light, the possibility of application for printing materials will be developed. After increasing contact angle by coating water and oil repellent on the titanium oxide thin film, images can be shaped by radiating light into pattern for changing surface of titanium oxide to be ultra hydrophilicity as decomposition of repellent. At that time, contact angle is 150 degree in water, 80 degree in oil, for not radiated aria, and is 0 degree in water and oil for radiated aria. Application for control technology of wettability keeps possibility of broader development to itself, not staying ability of self-cleaning and drip-proof. (NEDO)

  3. Growth hormone receptor deficiency in mice results in reduced systolic blood pressure and plasma renin, increased aortic eNOS expression, and altered cardiovascular structure and function

    DEFF Research Database (Denmark)

    Egecioglu, E; Andersson, I J; Bollano, E

    2007-01-01

    To study the role of the growth hormone receptor (GHR) in the development of cardiovascular structure and function, female GHR gene-disrupted or knockout (KO) and wild-type (WT) mice at age 18 wk were used. GHR KO mice had lower plasma renin levels (12 +/- 2 vs. 20 +/- 4 mGU/ml, P < 0.05) and inc....... These data suggest an important role for an intact GH/IGF-I axis in the maintenance of a normal cardiovascular system....

  4. Instantaneous heat flux flowing into piston top-land surface of D.I. diesel engine; DI diesel kikan no piston top land bu eno shunji netsuryusoku

    Energy Technology Data Exchange (ETDEWEB)

    Taguma, M [Zexel Corp., Tokyo (Japan); Inui, M; Enomoto, Y; Hagihara, Y [Musashi Institute of Technology, Tokyo (Japan); Koyama, T [Mitsubishi Motors Co., Tokyo (Japan)

    1997-10-01

    The thermal loads of the piston top-land surface in D.I. diesel engine during actual operation is not cleared. The authors fixed thin film thermocouples in the top-land center of a standard piston, and measured the instantaneous heat fluxes in that place. As a result, the authors made clear the thermal loads of the piston top-land surface in a cycle, and confirmed presence of the flame inflow to the piston top-land center. In addition, the authors made clear the thermal loads of the piston top-land surface in EGR operation. 4 refs., 8 figs.

  5. Inverse LMI control design method and its application to position keeping system; Gyakumondai LMI seigyokei sekkeiho no kaihatsu to ichi hoji system eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, I; Hayashi, T [Mitsubishi Heavy Industries, Ltd., Tokyo (Japan)

    1997-10-01

    Recently, with a progress of robust control such as H{infinity} control, the application of a control system design considering a robustness of controlled system fluctuation to real machinery became possible. In addition, LMI (Linear Matrix Inequality) control system design method is superior to conventional H{infinity} control, although including various difficult elements. The design algorithm permitting the robust control system design of non-linear problems by giving controlled systems was thus developed by introducing an inverse LMI control design method. This design algorithm determines the solution of models outputting the deviation between a target value and controlled variables by solving the simultaneous equations composed of a linear matrix inequality under an impulse response constraint condition and that under an H{infinity} norm constraint condition. This technique allows to give a pending step response as control condition. The technique was confirmed to be effective by its application to the position keeping control problem of marine floating structures. 6 refs., 11 figs., 2 tabs.

  6. Sildenafil citrate-restored eNOS and PDE5 regulation in sickle cell mouse penis prevents priapism via control of oxidative/nitrosative stress.

    Science.gov (United States)

    Bivalacqua, Trinity J; Musicki, Biljana; Hsu, Lewis L; Berkowitz, Dan E; Champion, Hunter C; Burnett, Arthur L

    2013-01-01

    Sildenafil citrate revolutionized the practice of sexual medicine upon its federal regulatory agency approval approximately 15 years ago as the prototypical phosphodiesterase type 5 inhibitor indicated for the treatment of male erectile dysfunction. We now provide scientific support for its alternative use in the management of priapism, a clinical disorder of prolonged and uncontrolled penile erection. Sildenafil administered continuously to sickle cell mice, which show a priapism phenotype, reverses oxidative/nitrosative stress effects in the penis, mainly via reversion of uncoupled endothelial nitric oxide synthase to the functional coupled state of the enzyme, which in turn corrects aberrant signaling and function of the nitric oxide/cyclic GMP/protein kinase G/phosphodiesterase type 5 cascade. Priapism tendencies in these mice are reverted partially toward normal neurostimulated erection frequencies and durations after sildenafil treatment in association with normalized cyclic GMP concentration, protein kinase G activity and phosphodiesterase type 5 activity in the penis. Thus, sildenafil exerts pleiotropic effects in the penis that extend to diverse erection disorders.

  7. Study on low permeable backfill materials. Backfill materials for waste disposal facilities; Koshisuisei juten zairyo ni kansuru kenkyu. Hoshasei haikibutsu shobun shisetsu eno tekiyosei kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Shiraishi, F; Konishi, M; Shiraishi, H [Okumura Corp., Osaka (Japan)

    1994-11-15

    A discussion was given on the fundamental properties of mortar made from cement mixed with silica fume. Four kinds of silica fumes with different particle size distributions were used. The following results were obtained: distribution of pores in mortar admixed with silica fume shifts to the smaller radius side; while the admixture increases the compression strength in mortar, the increasing trend varies depending on the kinds of silica fumes; this is thought to be caused from the difference in pozzolan activity between silica fumes; and the strength increase is caused by densification of the structure due to the pozzolan reaction that is generated after hydration, or in other words, decrease in the pore volume. The applicability of the mortar was investigated as a filling mortar to fill up clearances between wastes. It was found that the filling performance can be controlled by mixing silica fume at an adequate level and adjusting the viscosity. The diffusion coefficient for the admixed concrete is smaller by 30% to one digit than that for the ordinary concrete. High-performance water stopping concrete of 0.11 {times} 10{sup 4}cm {sup 2}/s at maximum was obtained. The concrete can be regarded as a material having superb filling and water stopping performances. 6 refs., 4 figs., 5 tabs.

  8. Application of photovoltaic generating system to electric power in large ship; Taiyoko hatsuden system no ogata senpaku eno oyo ni kansuru kento

    Energy Technology Data Exchange (ETDEWEB)

    Katagi, T; Ogawa, S; Nishikawa, E; Hashimoto, T [Kobe University of Mercantile Marine, Kobe (Japan); Ishida, K

    1996-10-27

    This paper describes the design of electric power system in a ship with photovoltaic power generating system, to examine applicability of the photovoltaic power generating system to the inboard power source. It also discusses effectiveness of the system for sea environment. At first, the actual route of a car carrier, meteorological data, and quantity of power consumption were picked up from the deck logbook and engine logbook. Then, the installation area of photovoltaic arrays, the quantity of photovoltaic power generation derived from the quantity of solar radiation, and the capacities of batteries and inverters were calculated, to design the electric power system in the ship with photovoltaic power generation system. Moreover, the NOx and SOx emissions were compared between the present power system and the usual power system using diesel power generator, to discuss the effectiveness of the present system for sea environment. Consequently, it was found that the emission of NOx was reduced by about 33% and the emission of SOx was reduced by about 28% compared to the usual power system. The effectiveness for sea environment was confirmed. 9 refs., 5 figs., 4 tabs.

  9. Economic development in India and the effect on the energy market in Asia; Indo no keizai hatten to Asia no energy shijo eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    As for the real GDP growth rate in India, the real GDP per capita during 1980-1993 increased 2.9% on annual average, but that in China did 8.0%. India has largely been behind China in the economic growth, but since the liberalization of economy in 1991, the Indian economy has remarkably been developed. It is predicted that the real GDP during 1993-2010 will be 5-6% on annual average. The difference in GDP increase between India and China is caused by differences in energy conservation, speed of conversion from non-commercial energy to commercial energy, serviceability of the industrial structure, etc. It is predicted that the primary energy consumption will grow on the level of China. The oil demand will grow 4-6% in China and 5-6% in India in 1993-2010, showing more increase in India. For oil import to India to exceed 1 million B/D in 2000, it is necessary to clear the ceiling of the international balance. For India to become a big country corresponding to its population size, a lot of difficulties should be overcome. 49 refs., 104 figs., 62 tabs.

  10. Management in ekonomija: ali je to dvoje ali eno? (Elementi in razumevanje postocializma in managerske revolucije = Management and Economy: Are They the Same Or Not?

    Directory of Open Access Journals (Sweden)

    Tonci Kuzmanic

    2007-03-01

    Full Text Available Author starts from important but suppressed equalisation of economy and management in post socialist and post capitalist circumstances which represents the very essence of post-modern global dominance (globalisation. An attempt to distinguish economy from management is connected with the appearance of neo-liberalism as the central argumentative machinery of management being connected with postmodern type of subjectivity. In comparison with the so-called »objective law« around which economy used to revolve (ideally, of course, management explicitly leaves this terrain and opens itself up to the subjective, even subversive activity at the level of global society. In that sense economy and management are operating on two radically different levels and we could even speak of two different entities which should be analytically distinguished. The very supposition for modern defence with regard to post-modern forms of dominance is exactly a possibility of differentiating between economy and management.

  11. ANALYSIS OF ACE, ACTN3, ENOS, PPARG, PPARA, HIF-15, PPARGC1B GENE POLYMORPHISMS FOR DETERMINATION A GENETIC PREDISPOSITION TO A VARIETY OF SPORTS

    Directory of Open Access Journals (Sweden)

    S. B. Drozdovska

    2013-05-01

    Full Text Available To establishing the possibility of assessing genetic iinherited predisposition to various sports, the differences in the distribution of genotypes of the complex polymorphisms in groups of athletes, specializing in sports with different types of energy supply of muscular work were studied. The paper examined the DNA 332 persons, of which 110 athletes involved in speed- power sports, 85 - in endurance sports , 51 - in sports that require a combination of strength and endurance, 86 - with no experience regular exercise.

  12. Field experiment for investigation of very shallow basement structure by micro-gravity survey; Microgravity tansa no gokusenbu kiban chosa eno tekiyo jikken

    Energy Technology Data Exchange (ETDEWEB)

    Oshita, K; Nozaki, K [OYO Corp., Tokyo (Japan)

    1997-10-22

    This paper illustrates the field experiment results in which micro-gravity survey was applied to investigation of very shallow basement structure between a few m and 10 m. Its applicability was discussed. In principle, the micro-gravity survey was conducted at the measuring points in a grid with 20 m pitch. Measuring points of 174 were used. The gravity system used for the measurements is an automatic gravimeter CG-3M made by the Scintrex. Survey results of P-wave reflection method conducted at the site using a vibrator focus were used as control data of micro-gravity survey. Consequently, change in the thickness of surface layer (earth filling) shallower than the depth of -10 m could be grasped as a plane. It was found that the micro-gravity survey is a useful method for the investigation of very shallow basement structure. Survey results by the reflection method could contribute to the determination of trend face at filtration and construction of density model as well as the geologic interpretation of gravity anomaly. As a result, reliability of micro-gravity survey and reflection method could be enhanced, mutually. 3 refs., 8 figs.

  13. Study on application of radar technique to explore mineral resources. 1. Sample test in laboratory; Radar ho no kosho tansa eno tekiyosei kento. 1. Shitsunai shiryo shiken

    Energy Technology Data Exchange (ETDEWEB)

    Chiba, A; Okada, K [Sumitomo Metal Mining Co. Ltd., Tokyo (Japan); Arai, E [Metal Mining Agency of Japan, Tokyo (Japan); Noguchi, K; Fujiwara, K [Waseda University, Tokyo (Japan)

    1997-10-22

    Dielectric constants of ore body and mother rock specimens taken from an epithermal gold deposit were measured in the frequency band of radar technique. Applicability of the radar technique to mineral exploration has been investigated by comparing measured results and ore showing. This paper describes the results. Measured results of the dielectric constants are summarized as follows. The specific dielectric constant in the forced dry condition did not depend on specimens. The specific dielectric constant in the water saturated condition increased with increasing the porosity. The conductivity increased with increasing the specific dielectric constant. The specific dielectric constant and conductivity increased with increasing the water content. The specific dielectric constant did not depend on types of rocks. The specific dielectric constant decreased with increasing the frequency. Difference of the specific dielectric constant in the water saturated condition decreased with increasing the frequency. The radar technique was applied to blind deposits. Since they were often in the ground water saturated zones, it was considered that the reflection at the boundary was enhanced with increasing the difference of specific dielectric constant between ore body and mother rock compared with that in unsaturated zones. 8 refs., 8 figs., 3 tabs.

  14. Simulation of emergency evacuation behaviour during disaster by using distinct element method; Hisaiji no gunshu hinan kodo simulation eno kobetsu yosoho no tekiyo ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Kiyono, J.; Miura, F.; Takimoto, K. [Yamaguchi Univ., Yamaguchi (Japan). Faculty of Engineering

    1996-04-21

    In this study, the possibility to apply the Distinct Element Method (DEM) to human evacuation behavior during a disaster was investigated, evacuation from rooms to outside though a passage or steps and a jam were examined as a basic consideration. The main results were obtained as follows: according to an analysis of evacuation behavior when elements of DEM were substituted for individual humans, the trace of individual people and force acting there to and the trace of the whole group so every time can be understood, analysis can also be conducted from local to macro. In the DEM analysis, a mutual distance between people and characteristics of behavior can be expressed well by introducing an imaginary bane into the elements. Evacuation time due to change of space shape can be compared and time series change of force acting on individual people can be understood according to the investigation on evacuation time difference due to shape change at the exit and entrance and the jam caused by the arch effect happened when the crowed flow appeared at the exit and entrance. 16 refs., 21 figs., 4 tabs.

  15. Sildenafil citrate-restored eNOS and PDE5 regulation in sickle cell mouse penis prevents priapism via control of oxidative/nitrosative stress.

    Directory of Open Access Journals (Sweden)

    Trinity J Bivalacqua

    Full Text Available Sildenafil citrate revolutionized the practice of sexual medicine upon its federal regulatory agency approval approximately 15 years ago as the prototypical phosphodiesterase type 5 inhibitor indicated for the treatment of male erectile dysfunction. We now provide scientific support for its alternative use in the management of priapism, a clinical disorder of prolonged and uncontrolled penile erection. Sildenafil administered continuously to sickle cell mice, which show a priapism phenotype, reverses oxidative/nitrosative stress effects in the penis, mainly via reversion of uncoupled endothelial nitric oxide synthase to the functional coupled state of the enzyme, which in turn corrects aberrant signaling and function of the nitric oxide/cyclic GMP/protein kinase G/phosphodiesterase type 5 cascade. Priapism tendencies in these mice are reverted partially toward normal neurostimulated erection frequencies and durations after sildenafil treatment in association with normalized cyclic GMP concentration, protein kinase G activity and phosphodiesterase type 5 activity in the penis. Thus, sildenafil exerts pleiotropic effects in the penis that extend to diverse erection disorders.

  16. Sočutno starševstvo in vzgojni stili v eno- in dvostarševski družini

    OpenAIRE

    Novak, Petra; Urh, Katarina; Presker, Martina

    2018-01-01

    Vzgoja otroka je ena izmed najtežjih vsebin življenja, v katero so starši neposredno vpleteni v procesu svojega delovanja. Otrok se rodi v že ustaljen sistem, ki si ga zakonca oziroma partnerja ustvarita še pred rojstvom otroka. Z vsakim novorojenim otrokom je treba vzpostaviti novo družinsko ravnovesje, da se bo družina lahko razvijala naprej. Pri razvoju otroka v družini ima najpomembnejšo vlogo odnos, ki ga starša vzpostavita z njim takoj ob njegovem rojstvu. Enako pomembno je tudi čustven...

  17. Všeobecný přenos daňové povinnosti jako krok k zásadní modernizaci DPH

    OpenAIRE

    Kohoutková, Růžena

    2014-01-01

    This final thesis deals with introduction of reverse charge mechanism of value added tax. The current system of value added tax has weaknesses, including carousel fraud, negative impact on cash flow of businesses and complicated rules to collect and manage the tax. Reverse charge mechanism is considered as a solution of existing weaknesses of current system. Although this regime is able to solve the mentioned weaknesses, it also has some disadvantages. Especially, it is a negative impact on c...

  18. Material revolution, and analysis of its impacts on the industry and technology of the next generation; Zairyo kakumei to jisedai sangyo gijutsu eno impact bunseki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1984-03-01

    This paper discusses possibility of advanced materials from seeds and expectations from needs; and also describes consistency between the seeds and needs, and possible impacts of these materials on the industry and technology of the next generation. Chapter 1 presents the promising results and eventual possibilities, based on the basic studies, of the advanced materials, e.g., fine ceramics, highly functional polymers, new alloys and composite materials. Chapter 2 analyzes the properties and functions attainable by these materials, and expected applicable areas; investigates their technical and economical possibilities; and also investigates their ripple effects on the technology and economy. Chapter 3 investigates the current situations of the demands in the automobile, chemical plant and energy areas, and problems involved in development of these materials for these areas. Chapter 4 investigates the measures to promote consistency between the seeds and needs of these materials, and impacts of these advanced materials on the related techniques, related industries and industrial structures. Chapter 5 summarizes the problems and measures narrowed down in Chapters 1 to 4 comprehensively for the above areas; and the measures to be taken by the private sector, and cooperation by the industrial, government and academic circles. (NEDO)

  19. In Vitro Activation of eNOS by Mangifera indica (Careless™) and Determination of an Effective Dosage in a Randomized, Double-Blind, Human Pilot Study on Microcirculation.

    Science.gov (United States)

    Gerstgrasser, Alexandra; Röchter, Sigrid; Dressler, Dirk; Schön, Christiane; Reule, Claudia; Buchwald-Werner, Sybille

    2016-03-01

    Mangifera indica fruit preparation (Careless™) activates the evolutionary conserved metabolic sensors sirtuin 1 and adenosine monophosphate-activated protein kinase, which have been identified as playing a key role in microcirculation and endothelial function. Here, an acute effect of a single dose of 100 mg or 300 mg Careless™ on microcirculation was investigated in a randomized, double-blind, crossover pilot study in ten healthy women to determine the effective dosage. Microcirculation and endothelial function were assessed by the Oxygen-to-see system and pulse amplitude tonometry (EndoPAT™), respectively. Cutaneous blood flow was increased over time by 100 mg (54% over pre-values, p = 0.0157) and 300 mg (35% over pre-value, p = 0.209) Careless™. The EndoPAT™ reactive hyperemia response was slightly improved 3 h after intake compared to pretesting with 300 mg Careless™. Furthermore, activation of endothelial nitric oxide synthase, as an important regulator for endothelial function, was tested in vitro in primary human umbilical vein endothelial cells. Careless™, after simulation of digestion, increased the activated form of endothelial nitric oxide synthase dose-dependently by 23% (300 µg/mL), 42% (1500 µg/mL), and 60% (3000 µg/mL) compared to the untreated control. In conclusion, the study suggests moderate beneficial effects of Careless™ on microcirculation, which is at least partly mediated by endothelial nitric oxide synthase activation. Georg Thieme Verlag KG Stuttgart · New York.

  20. Effects of fuel properties and oxidation catalyst on diesel exhaust emissions; Keiyu seijo oyobi sanka shokubai no diesel haishutsu gas eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Aihara, S; Morihisa, H; Tamanouchi, M; Araki, H; Yamada, S [Petroleum Energy Center, Advanced Technology and Research Institute, Tokyo (Japan)

    1997-10-01

    Effects of fuel properties (T90 and Poly-Aromatic Hydrocarbons: PAH) and oxidation catalyst on diesel exhaust emissions were studied using three DI diesel engines and two diesel passenger cars. (IDI engine) PM emissions were found to increase as T90 and PAH increased and could be decreased considerably for each fuel if an oxidation catalyst was installed. 5 refs., 9 figs., 3 tabs.

  1. Simple method for thick blade calculation. Part 2. Application to a thin blade; Kanbenna ichiatsuyoku keisanho (zokuho). Usui tsubasa eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Maita, S; Ando, J; Nakatake, K [Kyushu University, Fukuoka (Japan). Faculty of Engineering

    1996-04-10

    A source and QCM (SQCM) method has been developed, by which the blade performance can be calculated in a short time with satisfying the Kutta`s condition without repeating calculations even for the three-dimensional blade. Performances were calculated for the two-dimensional and three-dimensional blades. The SQCM has provided appropriate results. However, it was found that there are some problems for thin blades. In this study, the SQCM has been applied to a thin blade. The conventional eddy model equation of SQCM is not a continuous distribution in the strict sense. In this study, this problem has been modified. When point eddies with constant strength are in line continuously within the interval, the induced velocity at an arbitrary position can be expressed by the integral equation using a position of marginal point of the interval. This equation can be analyzed by the integral of influence coefficient. The position of marginal point of the interval is a position of control point determined by the QCM theory. As a result of the modification, it was found that the SQCM in response to a thin blade has provided precise calculation results stably even for an ultra thin blade with the blade thickness ratio of 0.001. 1 ref., 8 figs.

  2. Application of vertical electrical sounding combined with induced polarization method in ground water exploration; IP koka wo koryoshita hiteikoho suichoku tansa no chikasui chosa eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Kondo, M; Sakurada, H [Sumiko Consultants Co. Ltd., Tokyo (Japan); Suzuki, T [Hokkaido Development Bureau, Hokkaido Development Agency, Sapporo (Japan)

    1996-10-01

    For ground water exploration using vertical Schlumberger exploration method, measurement and analysis combined with induced polarization (IP) effect were conducted as trial. For the Schlumberger method, potential is measured at the center between potential electrodes during flow of dc current between current electrodes. In the case of vertical exploration, measurements are repeated with fixed potential electrodes by extending the distance between current electrodes. Ground water exploration was conducted using this method at Otaki village, Hokkaido. Geology of surveyed plateau consists of a basement of Pliocene tuffs and Quaternary Pleistocene sediments covering on the surface. For the results of analysis, four to seven beds were detected from the resistivity. The depth up to the lowest bed was between 25 and 85 m, the resistivity of each bed was between 9 and 8,000 ohm{times}m, and the polarizability was between 1 and 15 mV/V. Among these resistivity zones, it was judged that zones satisfying following three conditions correspond to coarse grain sediments saturated with ground water, and can be expected as aquifers; having resistivity ranging between 100 and 1,000 ohm{times}m, polarizability higher than 10 mV/V, and relatively large thickness. 11 refs., 6 figs.

  3. Three dimensional transient electromagnetic model study for fracture prediction from tunnel face; Sanjigen model keisan ni yoru TEM ho no tunnel zenpo tansa eno tekiyosei no kento

    Energy Technology Data Exchange (ETDEWEB)

    Wada, K; Tsutsui, T; Saito, A [Mitsui Mineral Development Engineering Co. Ltd., Tokyo (Japan); Hara, T [Toda Corp., Tokyo, (Japan); Zhdanov, M [University of Utah, UT (United States)

    1996-10-01

    In order to apply TEM model to fracture prediction at tunnel face, 3-D TEM model computation by FEM was conducted by installing a transmission loop on a tunnel face. MT field responses diffusing into the 3-D model were computed by time-domain difference calculus, and analytical precision was improved by introducing a staggered grid method. In the case where a low resistive zone exists before a tunnel face, time variance in diffused eddy current and induction current in the low resistive zone could be obtained. The difference in tunnel-axial transient curve (transient phenomenon curve in magnetic field) between uniform medium and low resistive zone models was based on the absorption process of diffused eddy current into the low resistive zone, and the expanding process of it toward the outside. Change in background condition could be predicted from the background and the ratio of transient curves every measurement. The detection limit of the low resistive zone was dependent on resistivity contrast, distance and geometry. Fluctuation in measurement due to noises and S/N ratio were also essential. 3 refs., 10 figs.

  4. Can spatial autocorrelation method be applied to arbitrary array shape; Kukan jiko sokanho no nin`i array eno tekiyo kanosei

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, H; Iwamoto, K; Saito, T; Tachibana, M [Iwate University, Iwate (Japan). Faculty of Engineering

    1997-05-27

    Methods to learn underground structures by utilizing the dispersion phenomenon of surface waves contained in microtremors include the frequency-wave number analysis method (the F-K method) and the spatial autocorrelation method (the SAC method). Despite the fact that the SAC method is capable of exploring structures at greater depths, the method is not utilized because of its stringent restriction in arrangement of seismometers during observation that they must be arranged evenly on the same circumference. In order to eliminate this restriction in the SAC method, a research group in the Hokuriku University has proposed an expanded spatial autocorrelation (ESAC) method. Using the concept of the ESAC method as its base, a method was realized to improve phase velocity estimation by making a simulation on an array shifted to the radius direction. As a result of the discussion, it was found that the proposed improvement method can be applied to places where waves come from a number of directions, such as urban areas. If the improvement method can be applied, the spatial autocorrelation function needs not be even in the circumferential direction. In other words, the SAC method can be applied to arbitrary arrays. 1 ref., 7 figs.

  5. Passive leg movement enhances interstitial VEGF protein, endothelial cell proliferation, and eNOS mRNA content in human skeletal muscle

    DEFF Research Database (Denmark)

    Hellsten, Ylva; Rufener, Nora; Nielsen, Jens J

    2008-01-01

    .05) in blood flow without a significant enhancement in oxygen uptake. Muscle interstitial fluid was sampled with microdialysis technique and analyzed for vascular endothelial growth factor (VEGF) protein and for the effect on endothelial cell proliferation. Biopsies obtained from the musculus vastus lateralis...... to cultured endothelial cells revealed that dialysate obtained during leg movement induced a 3.2-fold higher proliferation rate (P level fourfold above resting levels. VEGF mRNA and MMP-2 mRNA levels were...

  6. Utilization of hot exhaust water from power station to marine cultivation to increase product. Hatsudensho onhaisui no suisan zoyoshoku eno riyo

    Energy Technology Data Exchange (ETDEWEB)

    Chiba, K [The University of Tokyo, Tokyo (Japan). Faculty of Agriculture

    1990-07-20

    A large quantity of waste sea water, exhausted after having been used as cooling water in the thermal and unclear power stations, is higher by about 7 centigrade in temperature than that when taken in. Its utilization to the marine cultivation to increase product was explained in present status and points of problem. Among aquatic animals to like high temperature, those, valuavle from the viewpoint of fishery, are bred in different places. Upon breeding spawn to fry young fish, there are two methods of using it, ie., as species to be cultivated or let go in the water flow, and for the production of edible fish, high in price, such as sea bream, yellowtail and lobster. In any case, hot exhaust water gives effect of having fish quickly grow, and stimulating spawn and fry young fish in sexual ripeness. For example, ear shell and prawn are 5 to 6 times in elongation of shell length and 9 times even in weight, respectively, as large as those in case of natural sea water. While there are problems in costing required for pumping hot exhaust water up in the cultivation on land, and temperature adjustment in summer and winter, and water quality control in the crawl cultivation at the water takeout, which must be going to be solved for the commercialization and industrialization. 10 figs., 1 tab.

  7. Application of adaptive control to windmill power system by computer simulation. Simulation ni yoru tekio seigyo no furyoku hatsuden system eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Tomiya, T.; Matsuo, T. (Anan College of Technology, Tokushima (Japan)); Suzuki, T.; Kamano, T. (Tokushima University, Tokushima (Japan). Faculty of Engineering)

    1991-07-20

    A windmill power system using load DC generators having resistive load was subjected to discussions by simulation on development of a control method and its output characteristics to draw out the wind energy effectively even when the system characteristics are unknown. The control method used is a mountaineering method available among adaptive control methods. As a result of discussions, the effectiveness of the mountaineering method was confirmed in controlling the output either when the wind velocity is constant or variable. With respect to the control interval, which is a problem in carrying out a control, it was found that the control output stabilizes as the control interval is increased when the wind velocity is constant, but takes longer time before reaching a steady state output. When the wind velocity is variable, the increased control interval reduces the output slightly because of the load resistance change becoming coarser. It was also revealed that, on simulated natural winds, this control method can draw out more energy than by a fixed load resistance. 7 refs., 11 figs., 1 tab.

  8. Genetic algorithm based on virus theory of evolution for traveling salesman problem; Virus shinkaron ni motozuku identeki algorithm no junkai salesman mondai eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Kubota, N. [Osaka Inst. of Technology, Osaka (Japan); Fukuda, T. [Nagoya University, Nagoya (Japan)

    1998-05-31

    This paper deals with virus evolutionary genetic algorithm. The genetic algorithms (GAs) have been demonstrated its effectiveness in optimization problems in these days. In general, the GAs simulate the survival of fittest by natural selection and the heredity of the Darwin`s theory of evolution. However, some types of evolutionary hypotheses such as neutral theory of molecular evolution, Imanishi`s evolutionary theory, serial symbiosis theory, and virus theory of evolution, have been proposed in addition to the Darwinism. Virus theory of evolution is based on the view that the virus transduction is a key mechanism for transporting segments of DNA across species. This paper proposes genetic algorithm based on the virus theory of evolution (VE-GA), which has two types of populations: host population and virus population. The VE-GA is composed of genetic operators and virus operators such as reverse transcription and incorporation. The reverse transcription operator transcribes virus genes on the chromosome of host individual and the incorporation operator creates new genotype of virus from host individual. These operators by virus population make it possible to transmit segment of DNA between individuals in the host population. Therefore, the VE-GA realizes not only vertical but also horizontal propagation of genetic information. Further, the VE-GA is applied to the traveling salesman problem in order to show the effectiveness. 20 refs., 10 figs., 3 tabs.

  9. FY 2000 report on the survey of strategies for spreading renewable energy to developing countries; 2000 nendo tojokoku eno saisei kano energy fukyu senryaku chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    For Laos, Bangladesh, Malaysia and Vietnam, survey was conducted to promote the introduction and spread of renewable energy, mainly the photovoltaic power generation. Relating to the solar radiation, the amount in the four countries is much higher than that in Japan, 3.4kWh/m{sup 2}/day on average. The countries are areas suitable for the photovoltaic power generation. As to the wind velocity, the area where the wind velocity is higher than 5m/second, which enables the introduction of the wind power generation, is limited to the south/west of Bangladesh, Malaysia, and the coastal part and islands in Vietnam. The wind power generation is being used in Malaysia and Vietnam. As for the precipitation, it rains concentratedly in the rainy season, and therefore, it is difficult to stably secure energy through the year by the small-scale hydroelectric power generation. In Vietnam, however, resident individuals eagerly introduce the small-scale hydroelectric power generation, and about 20,000kW is now in operation. Concerning the introduction of the photovoltaic power generation, Bangladesh is in a state of working out a master plan, Laos is in a state of drawing up a plan for the spread, and Malaysia and Vietnam are in a state of the verification. (NEDO)

  10. Fundamental study on PV system for floating water power device `Mighty Whale`; Okiai futaishiki haryoku sochi `Mighty Whale` eno taiyoko hatsuden sochi tosai no kihon kento

    Energy Technology Data Exchange (ETDEWEB)

    Washio, Y [Japan Marine Science and Technology Center, Kanagawa (Japan)

    1996-10-27

    Studied in this paper is the use of a photovoltaic power generation system in combination with a wave energy conversion system to cover the latter`s weak point that it is available but intermittently. An offshore floating wave power device Mighty Whale (MW) is described, which will have a wave energy absorbing air chamber in the front and an anti-rocking float that looks like a whale in the rear. The movement, up and down and back and forth, of the water surface in the air chamber due to incident waves turns the power generator turbine. Out of the space available on the MW, a 200m{sup 2} area has been specified as the place suitable for the installation of solar cells capable of a maximum output of 20kW, for which specification the elevation of the sun has been taken into account. Batteries will routinely be supplied with power in parallel from the wave activated and photovoltaic devices, the supply will be limited when the batteries are fully charged, and an auxiliary power generator will be run in case of shortage. Power that can be provided by a 20kW solar array was calculated using actual data of sunshine on the slope for each array orientation, and required battery capacity was calculated for each solar array orientation. Important items for consideration for the insurance of reliability on the ocean include measures against salt damage, detailed designing of wiring, and coordinated operation of photovoltaic and wave activated power generation systems. 3 refs., 6 figs., 5 tabs.

  11. Thermal sensation vote simulation on the aged for bus air conditioning; Koreisha wo koryoshita onkan suchi simulation no bus kucho eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Takeuchi, H; Nishijima, H; Kitada, M; Shinma, A [Denso Corp., Aichi (Japan)

    1997-10-01

    The Numbers of passengers on the aged of sight-seeing buses about 5 times bigger than those of car passengers, have been increasing gradually. The former paper of DENSO in 1994 introduces quantitative method, which simulates passenger heat amount given by sun light at various solar positions. At this time, this paper introduces quantitative method, which simulates passengers thermal sensation skin temperature and also heat amount given by sun light at various solar positions. 5 refs., 8 figs., 2 tabs.

  12. Psychological influences for the driver by the changes of running circumstance; Jidosha no soko kankyo henka no driver eno shinriteki eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Okura, S; Yokomori, M; Yamaguchi, S [Meijo University, Nagoya (Japan)

    1997-10-01

    A driver is influenced psychologically by the cars proceeding on the street and the cars parked on the shoulder which come into the front view. The traffic accidents are caused when these influences put a lot of stress on a driver. In this report, we study the relations between the road traffic condition on the streets and the highway and the driver`s psychological and psysiological responses by using the driver`s front view, sweating and pulse rate. 6 refs., 8 figs.

  13. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus Otto; Mortensen, A; Nilas, Lisbeth

    2007-01-01

    -six female ovariectomized Watanabe heritable hyperlipidemic (WHHL) rabbits were randomised to treatment with 17beta-estradiol (17beta-E(2)), SoyLife 150 or control for 16 weeks. Ring segments of basilar artery (BA) and posterior cerebral artery (PCA) were mounted in myographs for isometric tension recordings...

  14. Recent progress in 'bioelectronics' research. Part 4. ; Recent trends in biosensor research and development. Baioerekutoronikusu eno michi (shorai wa saibogu mo). 4. ; Baiosensa gijutsu saizensen

    Energy Technology Data Exchange (ETDEWEB)

    Karube, I. (The Univ. of Tokyo, Tokyo (Japan). Research Center for Advanced Science and Technology)

    1994-02-20

    Biosensors are structured basing on a variety of principles. The latest trend of the research and development of biosensors is introduced to explain how the five senses such as taste sense and olfactory sense are detected. A biosensor is structured by combining biological element with a transducer which converts the result measured by the said element into electrical signal. Enzyme is most widely used at present. The enzyme recognizes a chemical substance very closely, and has a feature of promoting its reaction only. Chemical substances, therefore, can be distinguished if this enzyme is used. The principle of the biosensor is explained quoting the enzyme sensor as an example. Immune reaction and microbe reaction can be monitored by the similar principle to the above-mentioned principle. Sensors for measuring substances which are the bases of taste have been developed. Studies on smell sensors are also actively carried on, and the quartz oscillator is used mainly. 4 refs., 4 figs.

  15. Advancing aging society and its effect on the residential use energy demand; Shintensuru koreika shakai to kateiyo energy juyo eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    The paper analyzed various factors regulating the energy demand in aged households and viewed the future residential use energy demand in the aging society. In Part 1, based on the family budget survey annual report, a study was made of the energy consumption situation of aged people and the trend of the future residential energy consumption. In Part 2, a study was conducted based on survey data on the U.K., France, Sweden and Denmark. In Western countries which are the developed countries of aging, the energy conservation policy effectively worked for the space heating demand which is highest of all, and factors of the energy consumption increase by aging were absorbed. However, since in Japan, aging is rapidly advancing and further there are relatively more factors which connect to an increase in energy consumption in aged households as compared with Western countries, it is thought that Japan is in a situation where the energy consumption increases more often, influenced by aged households. 91 refs., 130 figs., 41 tabs.

  16. FY 1990 achievement report on the survey of possibility of introducing solar energy into buildings; 1990 nendo taiyo energy no kenchikubutsu eno donyu kanosei chosa seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-03-01

    This paper describes the achievements in fiscal 1990 on survey on possibility of introducing solar energy into buildings. Capacity of photovoltaic power generation facilities installed on an area of 1600 km{sup 2}, such as roofs of residential houses, schools and building may be calculated on a trial bases as 100 million kW at maximum. The capacity can replace 16% of the total demand and corresponds to 26.30 million kiloliters (9%) as converted to crude oil. If photovoltaic power generation is utilized on buildings of users, diversified technological criteria are required from the viewpoint of legislation covering wide range of architectural, electrical and disaster aspects. However, no direct and clear standards are available now, except for the electric power business law. What are urgently required are solar cell standardization, incentive subsidies, aging safety, institutions for electric power sale and purchase, and establishment of technological criteria. An utilization example survey revealed problems in array installation and size. Increased demand, mass production and cost reduction will create a favorable cycle. Introduction, proliferation, and acceleration under policies at public facilities which are expected of enlightenment and development into other areas will be the effective means for the purpose. (NEDO)

  17. Application of super workable concrete to main tower of cable-stayed prestressed concrete bridge. ; Kiba park grand bridge. PC shachokyo no shuto eno tekiyo. ; Kiba koen ohashi

    Energy Technology Data Exchange (ETDEWEB)

    Matsuoka, Y.; Shindo, T.; Sakamoto, A. (Taisei Corp., Tokyo (Japan))

    1993-08-01

    The Kiba Park Grand Bridge is a cable-stayed prestressed concrete (PC) bridge with a length of 186m. The main tower of this PC cable-stayed bridge consists of a pair of vertical columns with height of 60m and a beam connecting the columns. For the purpose of the advanced efficiency of construction without formwork and removal work and the improvement of durability, the precast buried formwork made of polymer impregnated concrete formwork was adopted. Approximate 650 cubic meter of super workable concrete was placed for the upper part ranging from 7th to 17th blocks of vertical columns and the beam. Blast furnace cement B and fly ash were used as binder. Naphthalenesulfonic acid type high performance water reducing agent and lignosulfonic acid type AE (air-entraining) water reducing agent were used as admixtures. Super workable concrete was mixed using forced double-axle mixers in the ready-mixed concrete plant. Satisfactory quality of the fresh concrete and strength of the hardened concrete were obtained. 2 refs., 11 figs., 3 tabs.

  18. Progress of admixtures and quality of concrete. 2. ; Approaches to ultra-high-strength concrete. Konwa zairyo no shinpo to concrete no hinshitsu. 2. ; Chokokyodo concrete eno approach

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, T. (Shimizu Construction Co. Ltd., Tokyo (Japan)); Abe, M. (Building Research Institute, Tsukuba (Japan))

    1994-02-15

    Ultra-high-strength concrete of 600 kgf/cm[sup 2] or more is reviewed. MDF (macro defect free) cement, spheroidal cement and mechanically stabilized cement have been developed for ultra-high-strength concrete, however, in general, DSP (densified system containing homogeneously arranged ultra-fine particles) technique is now usual in which a water-cement ratio is reduced by use of advanced air entraining and water reducing agents and cured concrete is densified by use of ultra-fine particles as admixture. Four kinds of substances such as naphthalene system and polycarboxylic acid system are used as air entraining and water reducing agents, and silica fume is used as ultra-fine particle admixture which can be effectively replaced with blast furnace slag or fly ash. Various use examples of ultra-high-strength concrete such as an ocean platform are found in the world, however, only some examples such as a PC truss bridge and the main tower of a PC cable stayed bridge in Japan. 22 refs., 10 figs., 2 tabs.

  19. Deposition of calcium carbonate into postglacial reefs: a test on a 'coral reef hypothesis'. Kohyoki no sangosho eno tansan calcium taiseki sokudo

    Energy Technology Data Exchange (ETDEWEB)

    Kayanne, H [Geological Survey of Japan, Tsukuba (Japan)

    1993-06-15

    This paper describes the following matters on changes in rates of deposition of calcium carbonate into postglacial coral reefs: Estimation was made on change in CaCO3 deposition in four coral reefs the data of which relating to all cross sections down to reef base have been acquired by drilling; the main deposition periods in the coral reefs formed in the postglacial period were five to six thousand years ago; the maximum deposition rate is estimated to be 2.7 [times] 10[sup 14] gC per one thousand years under an assumption that the total deposition amount in postglacial coral reefs is 1.2 [times] 10[sup 18] gC (converted to carbon amount); the recent deposition rate is (1/7.5) that of the former rate; from information obtained on submerged coral reefs, deposition amounts in coral reefs before 10,000 years ago are judged to have been smaller than those thereafter; and the above knowledges do not support the 'coral reef hypothesis' by Berger et al. that deposition of calcium carbonate into postglacial coral reefs has occurred from 15,000 years ago to 10,000 years ago. 30 refs., 2 figs.

  20. Studies of an application of mobile communication system to the private telecommunication network. Part 2. Trend of mobile communication systems and application to power utility; Idotai tsushin system no denryoku tsushinmo eno tekiyo. 2. Idotai tsushin system no genjo to denki jigyo eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Iwasaki, H. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1997-06-01

    This paper presents future prospects on development of different mobile communication systems operated by public communications operators, and discusses their application to power utilities. For automobile and cellular telephones, discussions are being made on the code division multiple access (CDMA) system which multiplexes and transmits information by using tally codes. Influence of noise decreases, and so does the need of retransmission due to code errors. Higher speed transmission than conventional systems may be expected. The system can be utilized for transmitting still images, such as for status of construction being carried out by power utility companies. For PHS, a discussion will start on 32 to 64-kbps transmission in fiscal 1997. By using the system together with an image compression technology, the system may be expected of utilization as a moving image transmission method for supporting site works by utility companies. The low and medium orbit satellite communications whose use is scheduled to start shortly can be utilized as construction and emergency disaster communication networks for mountainous areas and islands. Movements around the frequency band utilization and communication systems of IMT-2000 are becoming more complex. The Japanese proposal plans transmission of moving images, whereas more diverse utilization can be expected, such as for voice, data and images, in areas where no ground communication facilities are available for electric power operations. 16 refs., 4 figs., 3 tabs.

  1. Study of transmitting electric power utility communications in IP network. Transmission of existing electric power utility communications in best effort type IP network under delay constraint; Denryokuyo tsushin kaisen no IP mo eno shuyo kento. Chien jikan seiyakuka ni okeru best effort gata IP mo eno kison denryokuyo tsushin kaisen no shuyo

    Energy Technology Data Exchange (ETDEWEB)

    Miyake, H.

    2000-07-01

    Since IP network does best effort behavior fundamentally, it always generates delay time and delay variation. For this reason, IP network has been unsuitable to transmit real time data such as a voice. However, in recent years, the technologies which transmit real time data in IP network, e.g. VoIP, have spread. If it is possible to transmit the existing electric power utility communications in IP network using these technologies, total network cost can be reduced by the reduction of required bandwidth and the simplification of communication network systems. In this report, it is examined quantitatively whether end-end delay time of the existing electric power utility communications, e.g. on-line, telephone, carrier relay, CDT (cyclic digital transmission equipment), video conference, ITV(industrial television), is within their permission delay time when they are transmitted in the best effort type IP network. (author)

  2. Mina ja Mina Kunstihoones / Maarja Undusk

    Index Scriptorium Estoniae

    Undusk, Maarja

    2004-01-01

    Tallinna Kunstihoone nõukogu liige M. Undusk Tallinna Kunstihoones eksponeeritud Eesti Kunstnike Liidu aastanäitusest "Mina ja Teine", selle arutelust 16. I. Tallinna Kunstihoone näitusekorraldusest, kuraatoritest, suhetest EKL-ga

  3. Viking valitseb Rootsi-Soome liinil / Annika Matson

    Index Scriptorium Estoniae

    Matson, Annika, 1976-

    2004-01-01

    Viking Line'il Soome ja Rootsi vahelisel liinil reisijateveos suurim turuosa. Ettevõtte tegevusest, majandustulemustest. Kommenteerib Romet Kreek. Diagrammid: Soome ja Rootsi vahel sõitvatest laevadest pea iga teine on punane; Tõusuaastaid olnud vaid neli

  4. Prima Vista toob pargiraamatukogu

    Index Scriptorium Estoniae

    2005-01-01

    5. ja 6. mail 2005 toimub teine Tartu kirjandusfestival "Prima Vista". Avatakse pargiraamatukogu, kus esinevad 5. mail Jürgen Rooste, Merca, Aapo Ilves, Peep Ilmet, Marko Martinson, Jüri Kaldmaa, Jaan Pehk

  5. Baltic notes : the best in music

    Index Scriptorium Estoniae

    2005-01-01

    Heliplaatidest: "World Lullabies", Mielavs un Parcelaji "Paruna ar sevi", InCulto "Post Sov Pop", Lauri Saatpalu & Katrin Mandel "Sa Oled Hea", D.J.Critikal "Chapter One Ehk Teine Maitse", Jurga "Aukso Pieva", Terminaator "Go Live"

  6. The Swedish view : three countries, one market / Ben Nimmo

    Index Scriptorium Estoniae

    Nimmo, Ben

    2005-01-01

    Rootsi on Põhjamaadest suurim investor Balti riikides ning suuruselt teine investor Lätis ja Leedus. Majanduslikust koostööst Rootsi ja Baltimaade vahel. Tabel: Rootsi ja Baltimaade majandusnäitajad 2004. aastal

  7. The Constitutional Review Chamber of the Republic of Estonia : no. of the case 3-4-1-6-08 : date of decision 1 July 2008

    Index Scriptorium Estoniae

    2008-01-01

    Kohtulahendi 3-4-1-6-08 (Tallinna Ringkonnakohtu taotlus tunnistada põhiseadusega vastuolus olevaks lennundusseaduse § 71 lg 2 teine lause, millega on pandud ettevõtjale kontrolli teostamise kulude tasumise kohustus) tekst inglise keeles

  8. Telemehe salaelu / Jaan Ruus

    Index Scriptorium Estoniae

    Ruus, Jaan, 1938-2017

    2008-01-01

    George Clooney ja tema teine mängufilm "Ohtliku mehe pihtimused" ("Cofessions of a Dangerous Mind") : stsenarist teleprodutsent Chuck Barris'e memuaaride põhjal Charlie Kaufman : pearollis Sam Rockwell : Ameerika Ühendriigid - Saksamaa 2002

  9. Saatchi "Newspeak" - metropol defineerib meie keele? / Jaan Elken

    Index Scriptorium Estoniae

    Elken, Jaan, 1954-

    2010-01-01

    Näitusest "Newspeak: British Art Now Part 1" ("Uus kõnepruuk: Briti kunst nüüd. I") Saatchi galeriis Londonis, avatud 17. oktoobrini 2010. Näituse teine osa avatakse 27. oktoobril. Saatchi galeriist

  10. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2002-01-01

    Uutest plaatidest David Guetta "Just A Little More Love", Bond "Shine", Chris de Burgh "Timing Is Everything...", Diana Krall "Live In Paris", Richard Ascroft "Human Conditions", Rein Laaneorg "Teine elu", Leaves "Breathe", Derek Trucks Band "Jouful Noise"

  11. The challenge of three books of essays / Jüri Talvet

    Index Scriptorium Estoniae

    Talvet, Jüri, 1945-

    1997-01-01

    Arvustus: Kaplinski, Jaan. See ja teine. Tartu : TÜ Kirjastus, 1996; Priimägi, Linnar. Kommentaarium. Tln. : Perioodika, 1997. (Loomingu Rmtk.; nr. 1-3); Krull, Hasso. Katkestuse kultuur. Tln. : Vagabund, 1996

  12. World Economic Forum discusses Lithuanian prospects / Nathan Greenhalgh

    Index Scriptorium Estoniae

    Greenhalgh, Nathan

    2009-01-01

    Maailma Majandusfoorumil pakuti välja lahendusi Leedu majandusliku olukorra parandamiseks. Üks meetod majanduskriisist väljumiseks oleks liti devalveerimine ning teine koos Läti ja Eestiga Baltimaade arengupanga loomine

  13. Main: FBA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available teins Fuyuhiko Inagaki Graduate School of Pharmaceutical Sciences, Hokkaido University - Cells possess a pro...ved in the autophagy, but its processes and mechanisms remain to be elucidated -

  14. Yeast Interacting Proteins Database: YPR103W, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available tein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose sensors...gulated gene expression; interacts with protein kinase Snf1p, glucose sensors Snf

  15. Pjat smertnõhh grehhov Garri Pottera (s posledujushtshim razoblatshenijem) / Nikolai Karajev

    Index Scriptorium Estoniae

    Karajev, Nikolai, 1978-

    2003-01-01

    Vastukajasid J.K. Rowling'u Harry Potteri lugudele, kuna ekraanile jõuab teine nende ekraniseering "Harry Potter ja saladuste kamber" ("Harry Potter and the Chamber of Secrets") : režissöör Chris Columbus

  16. Nissan raputab Jaapani majandust / Alexander Garth

    Index Scriptorium Estoniae

    Garth, Alexander

    1999-01-01

    Jaapani suuruselt teine autotootja Nissan kavandab viie kodumaise tehase sulgemist, töötajate arvu vähendamist 21 000 inimese võrra ja loobumist pooltest oma varustajafirmadest järgmisel kolme aasta jooksul

  17. Endine linnaametnik saab pealinna suurpargi golfiväljakute rajamiseks / Silva Männik

    Index Scriptorium Estoniae

    Männik, Silva, 1974-

    2004-01-01

    Endise linnaametniku Tarmo Valgepea poolt juhitav MTÜ Eesti Golfi Liit sai Pirital asuva ligi 30 ha suuruse Lillepi pargi hoonestusõiguse sotsiaalmaa maksustamise hinnaga. Kaart: Tallinna suuruselt teine park muutub golfikeskuseks

  18. Ilves secures vote to continue in post / Karoliina Raudsepp

    Index Scriptorium Estoniae

    Raudsepp, Karoliina

    2011-01-01

    Eesti presidendiks valiti taas Toomas Hendrik Ilves. Teine presidendikandidaat Indrek Tarand sai 25 häält. Presidendivalimiste päeval toimus Toompeal presidendi otsevalimise pooldajate meeleavaldus. Väljavõte presidendi kõnest

  19. Deciphering groundwater quality for irrigation and domestic purposes

    Indian Academy of Sciences (India)

    for groundwater planning and management in the study area. It is not only the .... hydrochemical methods to assess the suitability of groundwater in .... levels and aid in the production of energy and pro- tein. ...... Alkali Soils; Agric Handbook 60.

  20. Luulud on lõbusamad kui reaalsus / Mari Laaniste

    Index Scriptorium Estoniae

    Laaniste, Mari, 1977-

    2006-01-01

    George Clooney ja tema teine mängufilm "Ohtliku mehe pihtimused" ("Cofessions of a Dangerous Mind") : stsenarist teleprodutsent Chuck Barris'e memuaaride põhjal Charlie Kaufman : pearollis Sam Rockwell : Ameerika Ühendriigid - Saksamaa 2002

  1. Kasvuhoone külmkapis ja ajareleega termos / Aarne Mäe

    Index Scriptorium Estoniae

    Mäe, Aarne, 1967-

    2006-01-01

    Priit Verlini muljeid Barcelonast Elektroluxi korraldatud disainiüliõpilaste konkursi Elektrolux Design Lab näituselt. Võitis Metin Kaplan Türgist, teine koht Brian Law Chuanile Singapurist, kolmas koht Eduardo Altamirano Segoviale Mehhikost

  2. Sony andis hirmutava kasumihoiatuse / Romet Kreek

    Index Scriptorium Estoniae

    Kreek, Romet, 1972-

    2005-01-01

    Maailma suuruselt teine tarbeelektroonika tootja Sony teatas, et selle aasta kasumiks tuleb 10 miljardit jeeni. Kasumihoiatus põhjustas Sony aktsia hinna kukkumise Tokyo börsil 3,6 protsenti, 3710 jeenile

  3. The nutritional composition ofSouth African eggs

    African Journals Online (AJOL)

    - tein ofanimal origin, all the essential amino acids, thiamine, riboflavin, pantothenic and folic acids, vitamin B 12 , biotin, vitamin D" vitamin E and phosphorus. Eggs are readily available and in- expensive and should be included in the diets of.

  4. Intellectual disability, oncogenes and tumour suppressor genes: the ...

    Indian Academy of Sciences (India)

    Identifying pathogenic copy number variations (CNV) involved in genomic disorders ... pathogenicity in the diagnosis of patients with intellectual disability, the ... tein has an essential role in the developing nervous system for the correct timing ...

  5. O voine i pamjati

    Index Scriptorium Estoniae

    2005-01-01

    Venemaa telekanal "Kultura" demonstreeris tsüklis "Teine Maailmasõda Euroopa kino silmade läbi" filme "Rooma - avatud linn", "Saksamaa. Aasta Null", "Kõik oma kodudesse", "Mary-Oktoober", "Läbi Pariisi"

  6. Reference: 575 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available proteins present in plasma membrane microdomains, appear to be involved in P. indica-induced growth promotion...tein is required for growth promotion and enhanced seed production mediated by the endophytic fungus Pirifor

  7. Kolm murdepunkti Eesti sisepoliitikas 1918-1934 / Ago Pajur

    Index Scriptorium Estoniae

    Pajur, Ago, 1962-

    2008-01-01

    Parlamentaarse demokraatia perioodi murdepunktidest. Esimene murdepunkt : 1920. aasta põhiseadus. Teine murdepunkt : kommunistide putšikatse. Kolmas murdepunkt : Suur depressioon. Tabelid: Erakondade esindatus Riigikogu teises, kolmandas ja neljandas koosseisus ; V Riigikogu erakondliku koosseise muutumine (kohtade arv)

  8. Popmuusika / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2006-01-01

    Uutest heliplaatidest Nits "Les Nuits", Bullfrog Brown "Snakes & Devils", "Motown remixed", "The Dukes Of Hazzard", Critical "Chapter One ehk Teine Maitse", The Dandy Warhols "Odditorium Or Warlords Of Mars", Solveig Slettahjell Slow motion Quintet "Pixiedust"

  9. Taassündinud selleks, et tappa / Olivier Roy ; tõlk. Külli-Riin Tigasson

    Index Scriptorium Estoniae

    Roy, Olivier

    2005-01-01

    Islami radikaliseerumisest, fundamentalismist. Autori väitel annavad kristlik ja islamiäärmuslus tunnistust üleilmastumise ajastu kultuurikriisist, islamiäärmusluse kasvulava on eeskätt noorte Euroopa muslimite teine põlvkond

  10. Akademitsheskije zaporozhtsõ / Nikolai Nortov

    Index Scriptorium Estoniae

    Nortov, Nikolai

    2005-01-01

    Autori kommentaarid Eesti 43 akadeemiku koostatud memorandumi tekstile ÜRO Peaassamblee, Euroopa Parlamendi ja Euroopa Nõukogu Parlamentaarse Assamblee poole pöördumiseks. Arvamust avaldab Mihhail Bronštein

  11. Web aitab nutikamaid firmasid / Peeter Stamberg

    Index Scriptorium Estoniae

    Stamberg, Peeter

    2003-01-01

    Interneti-lahendustesse investeerimine on aidanud paljudel ettevõtetel oma tulemusi parandada. Autor teeb ülevaate ajakirjas BusinessWeek avaldatud edukamatest internetiprojektidest. Lisa: Internetiäri teine laine

  12. Jack Sparrow on tagasi! / Timo Diener

    Index Scriptorium Estoniae

    Diener, Timo

    2006-01-01

    Gore Verbinski lavastatud piraadi-triloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" ("Pirates of the Caribbean : Dead Man's Chest"), peaosas Johnny Depp : Ameerika Ühendriigid 2006

  13. Bill Nighy - poolenisti homaar, poolenisti kalmaar / Bill Nighy ; interv. Margit Tõnson

    Index Scriptorium Estoniae

    Nighy, Bill

    2006-01-01

    Londonis esilinastus Gore Verbinski piraaditriloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" : Ameerika Ühendriigid 2006. Intervjuu briti näitlejaga, kes kehastas filmis Davy Jones'i

  14. Matkafoto 2002 / Kaupo Kikkas

    Index Scriptorium Estoniae

    Kikkas, Kaupo, 1983-

    2003-01-01

    Konkursi võitis Valdek Udris fotoga "Nägemus" Mont Blanci vallutamisest, teine oli Marten Kaevatsi foto "Priimusele" ja Peeter Saabase foto "Ajaviitemuusika", kolmanda koha said Aivo Kärbo, Arne Sarapuu ja Viktor Palm

  15. Uued plaadid / Koit Raudsepp

    Index Scriptorium Estoniae

    Raudsepp, Koit

    1999-01-01

    Uute plaatide 2 Quick Start "Teine pool, The Very Best Of 2QS", Jimi Hendrix "Live At Woodstock", Beverly Craven "Mixed Emotions", Ratt "Ratt", Todd Terry "Resolutions", Kenny Rogers "All The Hits And New Love Songs" tutvustus

  16. "Sõrmuste isandale" müüdi ette tuhandeid pileteid

    Index Scriptorium Estoniae

    2003-01-01

    Peter Jacksoni teine film "Sõrmuste isand : Kaks kantsi" ("The Lord of the Rings : The Two Towers") ja selle publiku-menust võrreldes ka teise Harry Potteri filmiga. Sama sõnum ka Linnaleht : Tartu, Pärnu

  17. The effect of autumn and spring planting time on seed yield and ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-03

    Jun 3, 2008 ... tein sources for human consumption, as well as for feeding animal in many ...... Protein quality of tropical food legumes. J. Sci. Technol. ... Nijhoff/Dr. W. Junk Publishers, The Hague, The Netherlands, pp 7-. 16. Hulse JH ...

  18. ORF Alignment: EHA1 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available tein [Entamoeba histolytica HM-1:IMSS] ... Length = 131 ... Query: 3 ... FEYLDHPADVILHSWGQNIIEAFENAAAGMFNFMSDLT...NIDATSYEEALVKFLDSWLCIF 62 ... FEYLDHPADVILHSWGQNIIEAFENAAAGMFNFMSDLTNIDATSYEEALVKFLDSWLCIF Sbjct...: 1 ... FEYLDHPADVILHSWGQNIIEAFENAAAGMFNFMSDLTNIDATSYEEALVKFLDSWLCIF 60 ... Query: 123 DQTHIHILLDI 133 ... DQTHIHILLDI Sbjct: 121 DQTHIHILLDI 131

  19. Evaluation of biochemical changes in unstimulated salivary, calcium ...

    African Journals Online (AJOL)

    TORNADO

    2012-01-26

    Jan 26, 2012 ... salivary, calcium, phosphorous and total protein during ... teins in saliva are important components and any chan- ... Sialochemical analysis .... quantities of protein utilizing the principal of protein-dye binding. Anal biochem.

  20. Venezia filmifestivali kimbutab staaripõud / Triin Tael

    Index Scriptorium Estoniae

    Tael, Triin

    2008-01-01

    Algas Veneetsia 65. filmifestival. Tanel Toomi lühimängufilm "Teine tulemine" on võetud Veneetsia filmifestivali lühifilmide programmi. Sellest sõnum "Eesti linateos võistleb lühifilmide programmis"

  1. A new leader for Lithuania's ruling party? / Kestutis Girnius

    Index Scriptorium Estoniae

    Girnius, Kestutis K.

    2011-01-01

    Irene Degutiene, kes on reitingult president Dalia Grybauskaite järel teine poliitik Leedus, tahab asendada erakonna Isamaaliit – Leedu Kristlikud Demokraadid praegust esimeest Andrius Kubiliust, kes on ka riigi peaminister

  2. Kunstikriitik Ants Juske sai doktoriks / Neeme Korv

    Index Scriptorium Estoniae

    Korv, Neeme, 1974-

    2003-01-01

    Tartu Kõrgema Kunstikooli rektor Ants Juske kaitses 7. veebruaril Tallinnas Kunstiakadeemias edukalt doktoriväitekirja, juhendajaks oli professor Boris Bernštein ning oponeerisid doktor Altti Kuusamo Soomest ja professor Peeter Tulviste

  3. Litva otsenila mirnõi atom / Vladimir Skripov

    Index Scriptorium Estoniae

    Skripov, Vladimir

    2006-01-01

    Vene-Ukraina gaasitüli on sundinud Leedut tegema korrektiive energiapoliitikas. On soovitatud Ignalina tuumajaama teise bloki seiskamise edasilükkamist kuni leitakse teine allikas energeetilise julgeoleku tagamiseks

  4. Genetics Home Reference: carnitine palmitoyltransferase II deficiency

    Science.gov (United States)

    ... PubMed Isackson PJ, Bennett MJ, Lichter-Konecki U, Willis M, Nyhan WL, Sutton VR, Tein I, Vladutiu ... 27 [updated 2017 Mar 16]. In: Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean ...

  5. Kuu plaat : Cardigans "Super Extra Gravity". Plaadid kauplusest Lasering

    Index Scriptorium Estoniae

    2005-01-01

    Heliplaatidest : Cardigans "Super Extra Gravity", Metsatöll "Terast mis hangund me hinge 10218", Ursula "Annamemenõu", Critikal "Chapter One ehk Teine Maitse", Robbie Williams "Intensive Care", Depeche Mode "Playing the Angel"

  6. Allosteric Regulation of Proteins

    Indian Academy of Sciences (India)

    interactions with other proteins, or binding of small molecules. Covalent .... vealed through structural elucidation of the protein in free and oxygen-bound forms .... stance, molecular dynamic simulation of glutamine binding pro- tein shows that ...

  7. 4 dnja v drugom formate / Dmitri Babitshenko

    Index Scriptorium Estoniae

    Babitshenko, Dmitri

    2007-01-01

    25.-28. okt. Teises Teatris toimuvast festivalist "Teine formaat 2. Sügishooaeg" (Drugoi format 2), mille programmis on muusikat, teatrietendusi, luulet, tantsulavastusi ja filme. Festivali organiseerija on Teise Teatri administraator Anastassia Kulkova

  8. ECB warns of risks involved in maintaining currency pegs

    Index Scriptorium Estoniae

    2009-01-01

    Euroopa Keskpank leiab, et Baltimaid võib ähvardada teine majanduskriis, kui valitsused ei võta vastu konkreetseid otsuseid. Euroopa Keskpanga hinnangul peaksid nad täiustama ka oma pangasüsteemi regulatsiooni

  9. Uudised : Kaunas

    Index Scriptorium Estoniae

    2005-01-01

    Baltic Multiplex Ventures, mille üheks osanikuks on BDG, avab Kaunases uue kobarkino ja meelelahutuskeskuse CINAMON. Esimene taoline keskus Eestis tuleb 2006.a. Tartusse ja teine Tallinna, uude Sakala keskusse

  10. Eesti Nõukogude Kunstnike Liidu asutamine / Jaak Kangilaski, Juta Kivimäe

    Index Scriptorium Estoniae

    Kangilaski, Jaak, 1939-

    2013-01-01

    4. jaanuaril 1943 leidis Jaroslavlis aset Eesti Nõukogude Kunstnike Liidu asutamise protseduur. Aasta pärast toimus Moskvas teine peakoosolek (kongress). Kunstilt nõuti sõja ajal teravat poliitilist kihutustööd

  11. 14. XI avati Pärnu uue kunsti muuseumi trepigaleriis...

    Index Scriptorium Estoniae

    2003-01-01

    Miamis elava Donald Koppeli sõja- ja pagemisfotode näitus. D. Koppeli teine fotoseeria juhib vaataja põgenemisteekonnale Eestist läbi Rootsi, Shotimaa, Walesi ja Portugali Madeira saarestiku üle Atlandi ookeani

  12. Võõrituse abil elulähedusse / Madis Kolk

    Index Scriptorium Estoniae

    Kolk, Madis

    2007-01-01

    Eesti Raadioteatri mõningatest kuuldemängudest : "Mardileib", "Kui mind ei oleks, oleks maailm teine", "Aabitsa kukk", "Elu sees", "Mavens ja Peder meedia rambivalguses", "Laste maailm", "Ahi", "Araabia öö"

  13. ETV teeb Tartu vanglas tantsufilmi

    Index Scriptorium Estoniae

    2005-01-01

    ETV-l valmib koos EFS-i ja Estinfilmiga kultuurikanali ARTE tellimusel tantsufilm "Teine" : režissöör Rene Vilbre : stsenarist Teet Kask : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir

  14. Helena Jonsdottiri tantsud kaameraga / Helena Jonsdottir ; interv. Margit Tõnson

    Index Scriptorium Estoniae

    Jonsdottir, Helena

    2005-01-01

    Tartu vanglas filmitud tantsufilm "Teine" ("Another"), režissöör Rene Vilbre. Islandi koreograaf ja stsenaariumi autor Helena Jonsdottir räägib filmi tegemisest, lisatud fakte koreograafi loomingust

  15. Под сенью комиксов в цвету /Николай Караев

    Index Scriptorium Estoniae

    Караев, Николай, 1978-

    2010-01-01

    Eesti I Koomiksibiennaal Kultuuritehase Polymer Kunstikonteineris Tallinnas alates 16. aprillist. Kuraatorid on Elisabeth Salmin ja Sandra Jõgeva. Esitletakse kollektiivselt loodud kunstnikuraamatut "Comic Magazine", toimub kirjanduslike kohtumiste seeria "Üks hullem kui teine" koomiksieri

  16. Rozhdestvenski blagovest prozvutshal v desjatõi raz

    Index Scriptorium Estoniae

    2005-01-01

    2004. a. jõulupreemiad "Rõõmukuulutus" pälvisid teiste hulgas Vene Draamateatri näitleja I. Nartov, loomingulise ühenduse "Teine Teater" asutaja ja produtsent N. Matshenene ja ajakirjanik ning teatrikriitik E. Kekelidze

  17. Logitech tutvustas juubelitooteid / Kuldar Kullasepp

    Index Scriptorium Estoniae

    Kullasepp, Kuldar, 1980-

    2006-01-01

    Vt. ka Postimees : na russkom jazõke 25. sept., lk. 14. Arvutite lisaseadmete tootja tõi turule kaks uut arvutihiirt: MX Revolution ja VX Revolution. Esimene neist on mõeldud laua-, teine sülearvutite kasutajatele

  18. Kohtla-Järve kunstnik maalis Koreas kaks hiigelkoopiat / Tiia Linnard

    Index Scriptorium Estoniae

    Linnard, Tiia, 1962-

    2004-01-01

    Aarne Hanni maalis Rogier van der Weydeni Johannese altarimaali 160 x 205 cm suuruse koopia, mis leiab koha Souli hiigelkirikus. Teine tundmatu maali koopia kujutas Jeesust ja Peetrust. Kunstniku kommentaarid

  19. Genome-wide identification, functional analysis and expression ...

    African Journals Online (AJOL)

    Fuyou Fu

    2013-07-24

    Jul 24, 2013 ... 1Chongqing Agricultural Technology Extension Station, Chongqing, 400037, China. 2Chongqing ... These data provide some clues for future molecular ... teins consist of one or two cytosolically oriented nubleo- tide-binding ...

  20. Fulltext PDF

    Indian Academy of Sciences (India)

    Integrated Ph.D Programme in Biological, Chemical and. Physical Sciences at ... phasis on an interdisciplinary approach. The course work is ... tein chemistry and engineering; cellular and applied immunology; molecular endocrinol- ogy and ...

  1. Prunskiene spurned for Kaliningrad visit / Milda Seputyte

    Index Scriptorium Estoniae

    Seputyte, Milda

    2005-01-01

    Leedu poliitikud avaldavad pahameelt põllumajandusminister Kazimiera Prunskiene Kaliningradi juubelipidustustele minemise üle, väites, et kui riigi presidenti pole kutsutud, siis ei tohiks ka keegi teine riiki esindada

  2. Meetodid opijärgse nakkuse vältimiseks / tõlkinud Kalle Klein

    Index Scriptorium Estoniae

    2010-01-01

    Kaks uurimismeeskonda on teatanud, et operatsiooniga seotud nakkusi saab vältida kahe erineva meetodi abil, millest üks on suunatud ninas kasvavale ja antibiootikumidele mittealluvale bakterile Staphylococcus aureus, teine aga nahal elavatele mikroobidele

  3. Plaadid / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2002-01-01

    Uutest plaatidest CAM "Soulshine", Will Smith "Born To Reign", Three Tenors "The Greatest Trios", Arnold Oksmaa "Teine Arnold", Norman Brown "Just Chillin", Safri Duo "Episode II New Edition", Rush "Vapor Trails", Vähi, Vetkalov, Tkatchenko "Truly Yours"

  4. Ööl vastu 13. II, oma 39. sünnipäeva eelõhtul, anti Tallinna Linnateatris üle kolleegi- ja muud preemiad

    Index Scriptorium Estoniae

    2005-01-01

    Kolleegipreemia said E. Nüganen (parim lavastus, "Tõde ja õigus. Teine osa"), Iir Hermeliin (parim kujundus, "Tõde ja õigus. Teine osa"), E. Tamm (parim naisnäitleja), A. Aadli (parim meesnäitleja), M. Koldits (parim kõrvalosatäitja), rekvisiitor D. Maripuu (parim lavastusala töötaja), M. Janisk (parim maja töötaja) ja A. Üksküla (eripreemia). Jagati ka juhtkonna preemiaid

  5. Resveratrol: A Multifunctional Compound Improving Endothelial Function

    OpenAIRE

    Li, Huige; F?rstermann, Ulrich

    2009-01-01

    The red wine polyphenol resveratrol boosts endothelium-dependent and -independent vasorelaxations. The improvement of endothelial function by resveratrol is largely attributable to nitric oxide (NO) derived from endothelial NO synthase (eNOS). By stimulating eNOS expression, eNOS phosphorylation and eNOS deacetylation, resveratrol enhances endothelial NO production. By upregulating antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) and suppressing the expression a...

  6. Vliv konečné apertury na přenos výkonu super-gaussovských svazků - analýza s využitím VirtualLabu

    Czech Academy of Sciences Publication Activity Database

    Stanke, Ladislav; Palatka, Miroslav

    2017-01-01

    Roč. 62, Jan (2017), s. 26-29 ISSN 0447-6441 R&D Projects: GA MŠk EF15_008/0000162 Grant - others:ELI Beamlines(XE) CZ.02.1.01/0.0/0.0/15_008/0000162 Institutional support: RVO:68378271 Keywords : beam clipping * super-Gaussian beams * simulation Subject RIV: BH - Optics, Masers, Lasers OBOR OECD: Optics (including laser optics and quantum optics)

  7. FY 1998 report on the results of R and D projects by local consortiums for immediate effects. Development of woody ceramics and application to building parts; 1998 nendo woody ceramics no sosei to kenchiku buzai nado eno oyo seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    The R and D project has been implemented for developing materials for, e.g., light, highly heat-insulating and energy-saving type building parts (e.g., roofing tiles and tiles), and light tableware having warmness of wood. The stock materials for china and porcelain, roofing tile clay, tiles and cement are dispersed with hollow microcapsules as the filler, which are of glassy balloons thermally treated to be highly functional, in order to develop superlight, highly heat insulating and high-strength materials. For development of the woody ceramic materials, the microcapsules are investigated for size, thickness, thermal shrinkage, fusibility, compressive stress, reinforcing mechanisms, and monodispersibility. Also investigated are optimization of material formability, development of commercial ceramics and evaluation of their marketability, decoration techniques, antimicrobial membrane materials and their immobilization techniques, development of antimicrobial ceramic tiles, evaluation of the antimicrobial membranes, evaluation of resistance to microwaves, and so on. The efforts are also directed to researches on fundamental techniques for woody tile clay, and R and D of the application techniques and products of the woody cement. (NEDO)

  8. Polimorfísmos genéticos de COX-1 y eNOS y hemorragias gastrointestinales: interacción con aire: Un estudio de casos y controles

    OpenAIRE

    Salado Valdivieso, María Inés

    2014-01-01

    La hemorragia gastrointestinal alta es un cuadro grave que está producido por medicamentos en un porcentaje elevado de los casos. Ciertos fármacos como los antiinflamatorios no esteroideos (AINE), los antiagregantes plaquetarios o los anticoagulantes orales, pueden dar lugar a una hemorragia que exija la hospitalización del paciente y, en ocasiones, provoque la muerte. Además de los fármacos, se han identificado otros factores que se asocian con la hemorragia gastrointestinal; entre ellos es...

  9. Studies of initial stage in coal liquefaction. Effect of decomposition of oxygen-functional groups on coal liquefaction; Ekika hanno no shoki katei ni kansuru kenkyu. 3. Gansanso kannoki no bunkai kyodo to ekika hanno eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Komeiji, A.; Kaneko, T.; Shimazaki, K. [Nippon Brown Coal Liquefaction Co. Ltd., Tokyo (Japan)

    1996-10-28

    Pretreatment of brown coal in oil was conducted using 1-methyl naphthalene or mixture of tetralin and 1-methyl naphthalene as solvent at temperatures ranging from 300 to 430{degree}C under nitrogen atmosphere. Effects of the solvent properties on the structural change of oxygen-functional groups (OFG) and coal liquefaction were investigated by means of quantitative analysis of OFG and solid state {sup 13}C-NMR measurement. When hydrogen transfer from solvent was insufficient, it was suggested that brown coal molecules loose their hydrogen to be aromatized. While, at lower temperatures ranging from 300 to 350{degree}C, hydrogen contained in brown coal molecules was consumed for the stabilization of pyrolytic radicals, and the deterioration of liquefaction was not observed. When hydrogen transfer from solvent was insufficient at higher temperatures above 400{degree}C in nitrogen atmosphere during pretreatment in oil, crosslinking like benzofuran type was formed by dehydration condensation of hydroxyl group in brown coal, to deteriorate the liquefaction, remarkably. The addition of donor solvent like tetralin decreased the formation of crosslinking like benzofuran type, which suppressed the deterioration of liquefaction. 8 refs., 5 figs.

  10. Application of plasma sprayed ceramic coatings to the base materials of the rotating disk in the centrifugal atomization process. Enshinryoku funmuho ni okeru kaiten enban eno ceramic yosha himaku no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Sato, T; Okimoto, K [Government Industrial Research Inst., Kyushu, Tosu, Saga (Japan); Yasutake, R [Koeiseiko Co. Ltd., Fukuoka (Japan)

    1992-07-08

    Applicability of the ceramic coating on the rotating disk was studied. In regard to the synthesis of Cu-based rapidly solidified powders, centrifugal atomization with molten Cu-24.6Sn was carried out using rotating disks sprayed with four kinds of sprayed ceramic coatings. It was found that atomization of Al203-40%TiO2 sprayed coating has been the best, and the yield ratio has been about 60 %. The melt temperature in case of Fe-based rapidly solidified metal powders, has risen above 1600[degree]C, and the required conditions for rotating disk have been very difficult to meet. The reason for it is thought that there has also been limitations regarding the functions of the characteristics like heat transfer, heat capacity, etc. Fe-24Cr-5Ni-1Mo 2 phase stainless steel powder has shown the most suitable trend among the seven kinds of disk materials examined for ZrO2 ceramic sprayed coatings. 6 refs., 5 figs., 2 tabs.

  11. Research of the elastic waves generated by a pulse laser. Excitation mechanism of elastic waves and application to nondestructive testing; Pulse laser de reikishita danseiha ni kansuru kenkyu. Danseiha reiki no mechanism to hihakai kensa eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Cho, H.; Takemoto, M. [Aoyama Gakuin University, Tokyo (Japan). College of Science and Engineering

    1994-07-20

    A bulk wave is generated when a pulse laser is irradiated to the material, and the characteristics of a Young`s modulus and Poisson`s ratio can be nondestructively estimated from the bulk wave. The generation mechanism of laser ultrasonic waves must be first clarified for such application. In this paper, fundamental research was conducted to study the generation mechanism of the elastic waves excited by a Q-switched Nd-YAG laser, and the generation method and characteristics of Rayleigh waves. The following result was obtained. A bulk wave is generated by the disk-like adiabatic expansion near the surface if the laser power is small when a spot-shape pulse laser was irradiated. A bulk wave is generated by the thin disk-like adiabatic expansion beneath the surface due to the thermal diffusion in the depth direction of a base material when the laser power becomes large. Moreover, a bulk wave is generated by the impact force due to abrasion and plasma when the power becomes still larger. The information on the bulk wave characteristics and Rayleigh wave was also obtained. 25 refs., 15 figs., 1 tab.

  12. Three-dimensional computer simulation at vehicle collision using dynamic model. Application to various collision types; Rikigaku model ni yoru jidosha shototsuji no sanjigen kyodo simulation. Shushu no shototsu keitai eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Abe, M; Morisawa, M [Musashi Institute of Technology, Tokyo (Japan); Sato, T [Keio University, Tokyo (Japan); Kobayashi, K [Molex-Japan Co. Ltd., Tokyo (Japan)

    1997-10-01

    The past study of safety at vehicle collision pays attention to phenomena within the short time from starting collision, and the behavior of rollover is studied separating from that at collision. Most simulations of traffic accident are two-dimensional simulations. Therefore, it is indispensable for vehicle design to the analyze three-dimensional and continuous behavior from crash till stopping. Accordingly, in this study, the three-dimensional behavior of two vehicles at collision was simulated by computer using dynamic models. Then, by comparison of the calculated results with real vehicles` collision test data, it was confirmed that dynamic model of this study was reliable. 10 refs., 6 figs., 3 tabs.

  13. New concept of combustion technology in small DI diesel engines. 4th Report. Effects of fuel injection rates on MK combustion; Kogata chokufun diesel kikan no shinnensho concept. 4. Funsharitsu no MK nensho eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, S; Matsui, Y; Kamihara, T [Nissan Motor Co. Ltd., Tokyo (Japan)

    1997-10-01

    A previous paper showed that EGR cooling and a low compression ratio which prolongs the ignition delay can expand the area of the new combustion concept. Experimental investigations were conducted in this research to examine the effects of the fuel injection rates, the injection pressure and the injection duration, on the exhaust emissions of an engine incorporating the MK concept The results showed that a higher injection pressure was effective in reducing NOx and particulate matter (PM) under MK combustion conditions. 10 refs., 9 figs., 1 tab.

  14. CFD simulation of three-dimensional motion of a vehicle with movable wings. Application to the keel of a racing yacht; 3 jigen undo suru kado yokufu shinko buttai no CFD simulation. Racing yacht keel eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Takada, N. [Mitsubishi Heavy Industries Ltd., Tokyo (Japan); Sato, T. [Tokyo Univ. (Japan)

    1998-12-31

    The computational fluid dynamics (CFD) is so remarkably developed in the various kinds of science and technology fields that it is utilized in aeroplane and other machines and structures. The introduction of Navier-Stokes equation into the fixed coordinate system makes it possible to perform the CFD simulation of vigorously moving body. Combining its procedure with the moving grid scheme enables us to maneuver a moving wing. A body with complicated shape like the keel of racing yacht is expressed by the multi-block grid to develop the CFD code corresponding to it. The simulation of forced motion, which is the first step of the motion simulation system, is applied to the keel of racing yacht to prove that the viscous flow field around the complicatedly shaped body. 6 refs., 17 figs., 2 tabs.

  15. Biochemical and toxicological impacts of persistent organochlorines on aquatic ecosystem. With particular attention to dioxins and their related compounds; Zanryusei yuki enso kagobutsu no suiken seitaikei eno eikyo. Toku ni dioxin rui ni chakumokushite

    Energy Technology Data Exchange (ETDEWEB)

    Iwata, H. [Hokkaido University, Sapporo (Japan)

    1998-07-10

    PCDDs, PCDFs and coplanar PCBs are organochlorine compounds which induce toxicological impacts on test animals, such as reduced weight, thymic gland dwarf, hepatotoxicity, immunotoxicity, developmental toxicity, carcinogenecity, teratogenicity and endocrine disturbance. They are generally referred to as dioxins. They are difficult to be decomposed in vivo, and tend to be more concentrated in higher organisms, placed at higher positions in the food chain. In aquatic ecosystems, they are observed to accumulate at high concentrations in fishes, fish-eating birds, claspers and whales. Therefore, there are growing concerns over their possible adverse effects on wild animals in aquatic ecosystems. This paper describes the effects of persistent organochlorine compounds on fishes, birds and aquatic mammals; and induction of cytochrome P450 of each organic species, caused by exposure to dioxins, and the induction examples in aquatic ecosystems. It also discusses whether or not the cytochrome P450 induction capacity of each organism species can be used as an index which explains difference between organism species in toxicological impacts of dioxins. 33 refs., 1 fig., 1 tab.

  16. Report on the survey in fiscal 1998. Systematic arrangement of environment technologies. 5 (Application of advanced technologies to environmental measures); 1998 nendo chosa hokokusho. Kankyo gijutsu ni kansuru taikeiteki seiri. 5 (sentan gijutsu no kankyo taisaku eno oyo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Global environment handling technologies were investigated and put into order. Universities often make research and development on themes that can be handled at laboratory levels or by simulations. Development at a practical application level is few. Technological seeds may include manufacture of hydrogen by water and steam decomposition using solar energy, and new synthesizing reactions utilizing solar energy. Included may also be urban type wind power generation, superconductive energy storage systems, biomass utilization, and natural energy utilization. Furthermore, CO2 recovery and utilization by liquefaction, alkaline metal heat power generation, and pulse power technologies can also be found. Studies on applying advanced technologies to environmental measures include composite materials, membrane separation, photo-catalysts, optical elements, porous bodies, functional polymers, bio-reactors, electron beams, and aeration. Private corporations are working noticeably on prevention of fluorocarbon and dioxin emission, PCB treatment and waste water treatment technologies, catalyst application technologies, recycling technologies, and incineration ash treatment and utilization technologies. (NEDO)

  17. FY 1997 report on the survey of potential impacts of enlarging ASEAN on political and economic systems in South East Asia; 1997 nendo chosa hokokusho (ASEAN kakudai no Higashi Asia no seiji keizai chitsujo eno eikyo chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This report surveys potential impacts of ASEAN (Association of South East Asian Nations) on inter-ASEAN affairs and its external relations when ASEAN will enlarge its members to include all nations in South East Asia, and thus fully represent the region. For this purpose, the survey was conducted on Vietnam, Myanmar, Laos and Cambodia, which joined in 1995, from the viewpoint of their economic and political system, and their relations with other member countries. The nature of ASEAN has gradually transformed, in which all the countries in the region have increased and internal economic issues have been tackled. It has an aim to stimulate inter-ASEAN trade and induce foreign direct investment into ASEAN as a whole by reducing import duties on intra-ASEAN trade. Underlying in these, new development is a concern about growing economic and military power of China. ASEAN solidarity will work an leverage against China should change toward worse, and ASEAN will function as a regional stabilization factor. ASEAN is needed for the stability of both in economic and political order in East Asia. Japan has to further promote its cooperation with ASEAN to help its solidarity as an association. 24 refs., 21 figs., 25 tabs.

  18. Síntese da 5-metil-2-oxabiciclo[3.3.1]nom-7-eno-3,6-diona.Determinaçăo estrutural de uma lactona bioativa isolada de otoba parvifolia /

    OpenAIRE

    Lenz, Cesar Antonio

    2003-01-01

    Orientador: Francisco de Assis Marques Tese (doutorado) - Universidade Federal do Paraná, Setor de Cięncias Exatas, Programa de Pós-Graduaçăo em Química. Defesa: Curitiba, 2003 Inclui bibliografia

  19. Bussijuhtide streigi ärahoidmiseks on täna jäänud viimane võimalus / Ingvar Bärenklau, Hindrek Riikoja, Eno-Gerrit Link

    Index Scriptorium Estoniae

    Bärenklau, Ingvar, 1967-

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke, 11. dets. 2007, lk. 6. Postimees : na russkom jazõke lüh. Transpordi ametiühingu ja autoettevõtete liidu delegatsioonid annavad hinnangu riikliku lepitaja ettepanekule palgatüli lahendamiseks, mis on viimane võimalus üldstreigi ärahoidmiseks. Lisa: Võimaliku streigi ajakava; Kuidas elab Tartu streigipäevadel?; Streigid ja streigiähvardused. Vt. samas intervjuud bussijuht Leo Sillastega: 6000-kroonist kuupalka peetakse kodus valejutuks; Pärnu bussijuhid on streigi osas ühel meelel

  20. Study of combustion in microgravity environments and application of the findings to combustors for industrial use; Bisho juryoku kankyoka ni okeru nensho kenkyu oyobi sangyoyo nensho kiki eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Sato, J. [Ishikawajima-Harima Heavy Industries Co. Ltd., Tokyo (Japan)

    1997-09-10

    Natural convection is one of the factors that make the elucidation of the combustion phenomenon difficult. Such being the case, it is necessary to utilize microgravity enviroments to learn fuel combustion characteristics and parameters governing the combustion phenomenon for the development of optimal burning appliances. The free-fall facility of JAMIC (Japan Micro Gravity Center) creates a microgravity field that lasts for 10 seconds, and helps perform various combustion-related researches. This report outlines the findings obtained thanks to the use of this facility. In a `study concerning the creation of sophisticated combustion technology,` the combustion of 50{mu}m fuel droplets (too small to involve natural convetion) in a jet engine combustor is simulated in a microgravity field using experimentally producible 1mm drops, and the relationship between the droplet burn time and pressure is disclosed. In addition, using a small combustion furnace, the behavior of a natural-size flame is estimated and the propagation speed of a carbon powder flame is studied. 6 figs.

  1. Synthesis and Characterisation of Tris(1-carboxyl-2-phenyl-1,2-ethyl eno dithiol enic-S,S') Tungsten Complex as Photo catalyst for Photolysis of H2O Molecules

    International Nuclear Information System (INIS)

    Fadhli Hadana Rahman; Rusli Daik; Mohammad Kassim; Khuzaimah; Wan Ramli Wan Daud

    2008-01-01

    Tris(1-carboxyl-2-phenyl-1,2-ethylenodithiolenic-S,S ' ) tungsten complex is one of the most promising photo catalyst to be used in photolysis of water to produce hydrogen. The first step of the synthesis involves a metathesis reaction of tetrapropylammonium bromide [((C 3 H 7 ) 4 N)Br] and ammonium tetrathiotungstate [(NH 4 ) 2 WS 4 ] to form a tetrapropylammonium tetrathiotungstate [((C 3 H 7 ) 4 N) 2 WS 4 ] (precursor). Then, the precursor was reacted with phenyl acetylenecarboxylic acid (C 9 H 6 O 2 ) to form tris(1-carboxyl-2-phenyl-1,2-ethylenodithiolenic-S,S ' ) tungsten complex (C 27 H 18 O 2 S 6 W). The infra-red, ultra violet/ visible (UV/ Vis) spectrum, nuclear magnetic resonance (NMR) and elemental micro-analysis of C, H, N and S agreed with the characteristic of the tris(1-carboxyl-2-phenyl-1,2-ethylenodithiolenic-S,S ' ) tungsten complex. The (W-S), (C-S) and (C=O) stretching frequencies were detected at 511, (1470 and 1035) and 1655 cm -1 , respectively. The 1 H NMR spectrum showed six protons in the complex. The 13 C NMR showed only 7 signals for carbon atom in the benzene ring, ethylene groups and carboxylic acid pendant group due to the symmetry of the molecules. The reaction yield was about 50 percent. Photolysis of acetone spiked H 2 O showed that the catalyst was able to produced 1.8 μmol/ h hydrogen. (author)

  2. Electrolyte loss mechanism of molten carbonate fuel cells. 2.; Application to the cell with matrix electrolyte layer; Yoyu tansan`engata nenryo denchi ni okeru denkaishitsu loss kiko ni tsuite. 2.; Matrix gata denkaishitsuso wo yusuru denchi eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Sonai, A; Murata, K [Toshiba Research and Development Center, Kawasaki (Japan)

    1993-11-01

    A single cell of molten carbonate fuel cell using a matrix electrolyte layer fabricated by using the doctor blade process has been operated for several thousand hours, measured of electrolyte loss amount, and analyzed by using a new electrolyte loss mechanism. The result may be summarized as follows: according to a result of measuring the matrix layer pore distribution, the average pore size has increased little by little; pores with diameters greater than 2 {mu}m at which no electrolyte retention becomes possible remain at nearly constant ratio up to 1800 hours, but increased after 2500 hours; the pore capacity in ports with the largest electrolyte retaining diameter of 2 {mu}m or less showed slight decrease with time in the anode, and an initial decrease followed by flatness, and then a sharp decrease after 1800 hours in the matrix layer; the electrolyte loss measurement values have remained nearly constant for 25 hours to 1800 hours, but increased sharply thereafter; and the electrolyte loss in this single cell due to pore capacity decrease in pores as power generating parts with diameters smaller than 2 {mu}m was explained quantitatively by a new electrolyte loss mechanism. 11 refs., 6 figs.

  3. Fázově koherentní přenos stabilní optické frekvence Brno - Praha po optickém vlákně délky 306 km

    Czech Academy of Sciences Publication Activity Database

    Číp, Ondřej; Čížek, Martin; Pravdová, Lenka; Hucl, Václav; Řeřucha, Šimon; Hrabina, Jan; Mikel, Břetislav; Lazar, Josef; Vojtěch, J.; Smotlacha, V.

    2016-01-01

    Roč. 61, 4-5 (2016), s. 91-97 ISSN 0447-6441 R&D Projects: GA ČR GB14-36681G; GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 Keywords : laser * optical fiber * optical and frequency transfer * optical frequency standard Subject RIV: BH - Optics, Masers, Lasers

  4. Epidemias de ferrugem asiática no Rio Grande do Sul explicadas pelo fenômeno ENOS e pela incidência da doença na entressafra

    Directory of Open Access Journals (Sweden)

    Claudinei Antonio Minchio

    Full Text Available RESUMO Estudou-se a interferência da variabilidade da variabilidade climática e do número de relatos de ferrugem asiática na entressafra sobre a epidemia da doença no estado do Rio Grande do Sul, durante 11 safras agrícolas. A ocorrência da doença foi obtida a partir de relatos apresentados no Consórcio Antiferrugem e dividida entre os períodos “Safra”, “Entressafra” e “Ano Todo”. Os dados das epidemias foram testados para correlação, via regressões polinomiais lineares e quadráticas, com as variáveis climáticas: precipitação pluvial, índice padronizado de precipitação - SPI, índice de oscilação sul - IOS e anomalia da Temperatura na Superfície do Mar - SST no Oceano Pacífico Tropical e número de ocorrências da doença no período de entressafra. Os índices que melhor explicaram a variação das ocorrências da doença para o período “Ano Todo” foram o SST e IOS, com coeficientes de determinação (R2 de 0,94 e 0,59, respectivamente. As ocorrências de ferrugem no período “Entressafra” influenciaram positivamente nas ocorrências no período “Safra”.

  5. Application of waste tires to asphalt pavement. Improvement of adhesion of asphalt with rubber particles; Haitaiya no asphalt hoso eno tekiyo. Asphalt to gomu ryushi no fuchakusei no kairyo ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Nakaoka, I. [Kansai Electric Power Co. Inc., Osaka (Japan)

    1994-10-10

    With an objective to apply waste tires to asphalt pavement, an experiment was carried out to improve adhesion of asphalt with rubber particles by using polymers. The state of interface on rubber particle and asphalt mixture was observed by a scanning electron microscope. As a result, it was found that the surface of untreated rubber particles is not bonded with the asphalt, but polymer treated mixture was found to have the affinity of rubber particles with asphalt improved. Tensile bonding strength was tested on rubber plates and asphalt. The result revealed that the polymer-reformed mixture has two times as large tensile bonding strength as that of the untreated mixture. With regard to the characters of asphalt mixture mixed with rubber particles, the stability shows a decreasing trend as compared with the standard asphalt concrete, but presents an excellent performance in wear. The fluidity resistance value is inferior to the standard, but not as great as presenting a problem under normal using environment, where its applicability as a road paving material was verified. 4 figs., 2 tabs.

  6. FY1995 study of ultra high resolution laser and microwave spectroscopy and its application to future standards; 1995 nendo choseimitsu laser micro ha bunko to sono jisedai hyojun eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    Ultra high resolution spectroscopy is carried out in a range from microwave to ultraviolet using lasers with laser cooling, saturated absorption, 2 photon absorption and ion trap, and optical parametric oscillation is studied for optical frequency measurement. A temperature of 10 {mu}K and that of 150 mK were obtained for Cs atoms and a single Yb ion, respectively. A stability of 3.3 x 10{sup -12} was obtained for iodine stabilized YAG lasers. A saturation signal of C{sub 2}H{sub 2} was observed during wore than 9 months. An optical parametric oscillator, which was fabricated to measure an optical frequency, was probed to work continuously more than 3 hours. (NEDO)

  7. Development of a readily recyclable sound insulation material made of polyester fibers. Application of the PET fibers from plastic bottles; Recycle kanona jidoshayo polyester sei kyuon zairyo no kaihatsu. Shiyozumi pet bottle zai no insulator zai eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Nemoto, K; Watanabe, K; Sugawara, H; Minemura, Y [Nissan Motor Co. Ltd., Tokyo (Japan)

    1997-10-01

    We have developed new polyester sound-absorbing materials made of fine and modified-cross-section polyester fabric. They provide noticeably higher sound-absorbing performance than traditional materials. Another feature of the new materials is their excellent recyclability since they are made of polyester. Application of the new materials to the dash silencer and the floor carpeting produced a great improvement in sound-insulation performance with less weight. 2 refs., 7 figs.

  8. Development II of ion current combustion control system. Application to the lean burn system of the micro vehicle engine; Ion denryu wo mochiita nensho seigyo system no kaihatsu. 2. Keijidosha engine wo mochiita lean burn eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Iida, T; Asano, M; Kajitani, M; Kuma, t; Morinaga, Y [Daihatsu Motor Co. Ltd., Osaka (Japan)

    1997-10-01

    The lean bum engine has not been introduced into the micro vehicle so far because of the low cost requirement and small displacement, in spite of it is one of the effective solutions which increase the fuel economy. By the way, we had already reported in the former paper that we developed the ion current combustion control system the architecture of which is cheaper than the other system. In this paper, we described the way how the above system is applied to the lean burn system of the micro vehicle engine and also we reported the gain of fuel economy and the exhaust emission level. 5 refs., 11 figs., 2 tabs.

  9. Life cycle assessment of supercharger for automotive use. Small displacement, high charging pressure engine and environmental load; Jidosha tosaiyo supercharger no life cycle assessment (LCA hyoka). Shohaikiryo kokakyu engine no kankyo eno yasashisa

    Energy Technology Data Exchange (ETDEWEB)

    Takabe, S; Sonoya, T; Hara, M [Ishikawajima-Harima Heavy Industries Co. Ltd., Tokyo (Japan)

    1997-10-01

    In resent years environmental conservation requires low fuel consumption and low emission engine. And environmental load of every car life stage (production, using, abolition) is considered. Life Cycle Assessment of supercharging small displacement engine is reported, compared with natural aspirated engine as same maximum torque and maximum power as supercharging engine. 6 refs., 8 figs., 3 tabs.

  10. Strain measurement of wheel by a super-small size strain history recorder and its application to fatigue design; Chokogata jitsudo hizumi keisoku sochi ni yoru wheel no hizumi keisoku to hiro kyodo sekkei eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, Y [Kyushu University, Fukuoka (Japan); Mineki, K; Wakamatsu, K [Central Motor Wheel, Tokyo (Japan); Morita, T

    1997-10-01

    A very small strain history recorder based on the rainflow method has been developed and applied to strain measurement of car wheels under several road tests. Various strain amplitude histogram data under mountain road, city road and high-way were acquired by the recorder for various types of wheels. The data were studied from the viewpoint of random fatigue and the fatigue damages were evaluated by Miner`s rule. The results of the damage evaluation were used for the improvement of shapes of wheels. 2 refs., 6 figs., 1 tab.

  11. Achievement report on the research on possibility of expanding application of information technology to automobile production line; Jidosha seisan kotei eno joho tsushin gijutsu tekiyo kakudai kanosei ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Before submitting propositions on important technological tasks to be generated in the automobile production line upon application of IT (information technology), a questionnairing is implemented to know the actualities of the line for the purpose of planning a proper merger of IT and the manufacturing technology development capability of the IT accepting site. The questionnaire, aiming at the embodiment of the goals of the projects 'IT related technical tasks relative to its helping human creation of new technologies and enhancement of human ability to make improvement' and 'Technical tasks of developing technical skills into technologies,' asks engineers about the actualities of knowhow at automobile production lines and its development into technologies, about how they take care of what they learned from their predecessors, and about their expectations from and views and opinions toward IT introduction and utilization. It is important to efficiently utilize IT as a means to solve problems involving the knowhow related training on the production line and the handing down of the acquired knowhow to the juniors. Since individual workers' knowhow assumes a certain role at each stage of the automobile production line and since it is necessary to extract the maximum from each worker and to utilize the fruit for improvement on productivity and work efficiency, it is indispensable to efficiently merge the knowhow with IT. (NEDO)

  12. Study on electrical monitoring of fill-up improvement. Utilization of resistivity data for soil compaction management; Hiteiko mitsudo kanri ni kansuru kisoteki kenkyu. Moritsuchi seko kanri eno hiteiko yuko riyo

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, N; Hiramatsu, W [Dai Nippon Construction, Gifu (Japan); Sugano, T [Kyoto University, Kyoto (Japan). Faculty of Engineering

    1996-10-01

    At present, measurement of wet density and water content by radio isotope (RI method) is used for filling management. It is supposed for filling in road construction that putting-out thickness of banking material reaches nearly 1m in the future rapid construction, however, use of RI method is difficult because of inserting and installing of a radiation source rod, large measuring apparatus, and the legal regulation on radioactivity intensity. Application of resistivity measurement was thus considered. Since resistivity has the same parameter as compaction curve, and monotonously decreases with an increase in water content by volume, it is applicable as construction management index. The management reference of resistivity (Rm) is set by plotting of a compaction curve and corresponding resistivity curve, determination of the reference resistivity (Ro) corresponding to a maximum dry density and optimum water content ratio, and setting of the allowance to Ro according to soil or application. It was clarified that Rm is applicable as set for every filling material. 1 ref., 10 figs., 2 tabs.

  13. Application of magnetotelluric method to hydrocarbon exploration. Case study of MT survey in Yurihara oil and gas field, Akita prefecture; MT ho no kokunai sekiyu tanko eno tekiyo. Akitaken Yurihara chiiki ni okeru jikken chosa kekka

    Energy Technology Data Exchange (ETDEWEB)

    Mitsuhata, Y; Matsuo, K; Ishikawa, H; Tanaka, H; Nakagami, K [Japan National Oil Corp., Tokyo (Japan). Technology Research Center

    1996-10-01

    The experiment and survey were carried out to verify the effectiveness and limitation of MT method. The previous seismic reflection method is unsuitable for steep terrain and over-folded complicated geological structure. In such difficult areas, application of MT method is increasing as complement or substitution for the reflection method. However, the evaluation of resistivity structure interpretation for every area is slightly difficult because of lack of data, poor quality of data, and no verification data at boreholes. Consequently, the survey was again carried out in well-known Yurihara oil and gas field, Akita prefecture whose geological structure has been surveyed in detail at boreholes, by adding new 32 sites, 22 sites on the south side and 10 sites on the north side of the measuring line extending from east to west. As a result, it was clarified that the lower high-resistivity layer found by MT method is equal to the Nishikurosawa basaltic layer, and basalt extends to the west in the north area. This result can`t be simply compared with the previous geological profile. 8 refs., 8 figs.

  14. Hodnocení efektivity vynaložených prostředků na reklamu během sportovních přenosů

    OpenAIRE

    Kozlová, Natálie

    2015-01-01

    Title: Evaluation of the effectiveness of advertising expenses during sport broadcasts Objectives: The main goal of this work is to compare the amount of money invested into the commercial advertisement during sport broadcasts at the ČT sport channel with viewer ratings. The used metric is a coefficient computed as a ratio between the viewer rating of the particular broadcast and the corresponding advertisement cost, normalized using Cost per Thousand method. Methods: Analysis of the secondar...

  15. Advanced mineral and lithological mapping using high spectral resolution TIR data from the active CO2 remote sensing system; CO2 laser wo mochiita kosupekutoru bunkaino netsusekigai remote sensing data no ganseki kobutsu shikibetsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Okada, K [Sumitomo Metal Mining Co. Ltd., Osaka (Japan); Hato, M [Earth Remote Sensing Data Analysis Center, Tokyo (Japan); Cudahy, T; Tapley, I

    1997-05-27

    A study was conducted on rock/mineral mapping technology for the metal ore deposit survey using MIRACO2LAS, an active type thermal infrared ray remote sensing system which was developed by CSIRO of Australia and is now the highest in spectral resolution in the world, and TIMS of NASA which is a passive type system. The area for the survey is the area of Olary/Broken Hill and Mt. Fitton of Australia. A good correlation is seen between the ground reflectance measured by MIRACO2LAS and the value measured by the chamber CO2 laser of rocks sampled at the above-mentioned area. In case that the width of spectral characteristics is below 300nm, the inspection ability by MIRACO2LAS`s high spectral resolution is more determined in mineral mapping as compared with TIMS which is large in band width. Minerals mapped using MIRACO2LAS are quartz, talc, amphibole, hornblende, garnet, supessartine, dolomite, magnesite, etc. 4 refs., 3 figs.

  16. Sterilization using ozone-ion gas sterilization using ozone-ion gas for mattresses used by the elderly; Ozone ion kunjoho ni kansuru kenkyu. Ozone ion kunjoho no zaitaku kaigoyo mattress mekkin eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Mikami, H.; Suzuki, A.; Hamasaki, H. [Shinryo Corp., Tokyo (Japan); Ishikawa, S.; Miyata, M.; Nanba, T. [Kitasato Univ., Tokyo (Japan); Sueyoshi, K. [Mitsubishi Research Institute Inc., Tokyo (Japan)

    1999-05-25

    The new generation of Japanese is facing an aging society: the number of young people is decreasing dramatically, while the number of elderly is increasing. Consequently, care for the elderly has become a big problem. One particular problem area we`ve investigated is the use of unclean mattresses by the bedridden elderly. These mattresses provide an all too excellent environments for the growth of microbes. We measured the density of microbes on the cover of mattresses at 775cfu/cm{sup 2} and in the bed filling at 136cfu/g. The dominant species of microbes were MRSA and Bacillus. We used MRSA, Bacillus subtilis and Escherichia coli as biological indicators and tested whether our system using ozone-ion fumigants was applicable for sterilization of mattresses. The survival ratio of MRSA were 10{sup -8} {approx} 10{sup -9}, 10{sup -7} {approx} 10{sup -8} for B. subtilis and below 10{sup -8} for E. coli. These experiments made it clear that our system is applicable for sterilization of the mattresses used by bedfast elderly. (author)

  17. Adoption of a fiber reinforced plastics (FRP) inner cylinder for the chimney stack at the Wakayama power plant; Wakayama hatsudensho entotsu eno FRP sei naito no saiyo ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Hirakawa, M. [Kansai Electric Power Co. Inc., Osaka (Japan)

    2000-05-10

    The chimney stack at the Wakayama power plant is planned to adopt a fiber reinforced plastics (FRP) inner cylinder having greater cost reduction effect than a steel made inner cylinder. The present study has performed different experiments on FRP under the gas emitting condition at the Wakayama power plant, and discussed structural plans based on the result therefrom. The Wakayama power plant has the waste gas raised in temperature because of difference in the power generation system. Therefore, physical constant verification tests and heat resistance tests were performed on FRP to verify its applicability. Structural discussions to pursue economy were given on the 'joints' and 'suspension points' (supporting points to suspend the FRP inner cylinder) being the major FRP structures based on the actual record at the Nanko power plant. The tests have placed focus on the 'heat resistance', and different experiments were carried out upon selecting two kinds of novolak-based vinyl ester resins. As a result, applicability of the FRP made inner cylinder to high waste gas temperatures was verified, and so were various property values to be used for designing the cylinder. Structural design was performed by applying design safety factor to the derived property values, and the basic structural plan was established for the FRP inner cylinder. (NEDO)

  18. Report for fiscal 2000 investigations on Activities Implemented Jointly in China and promotion of transfer to CDM; 2000 nendo Chugoku ni okeru kyodo jisshi katsudo oyobi CDM eno iko suishin chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    It is purposed to promote the Activities Implemented Jointly (AIJ) and the clean development mechanism (CDM) intended of reducing carbon dioxide emission amount in China. Investigations have been performed on China's environment and energy problems, CDM assignments and transfer means, and project candidates. China emits a great amount of CO2 due to coal combustion, and the CO2 emission is anticipated to increase from the coal burning thermal power generation that may continue into the future. Countermeasures for the thermal power department as the object are required. Since 1997, China has been performing the AIJ project with Norway, Japan, and America, wherein such projects have been implemented with Japan as the dry coke fire extinguishing facility model project, the energy saving model project using alloy iron electric furnaces, and the model project to utilize effectively the refuse combustion waste heat. China is one of the countries in which the greenhouse effect gas emission reducing project can be performed at a minimum cost, who will be the important party in the CDM performed by the developed countries to achieve their obligation to reduce the greenhouse effect gas emission. (NEDO)

  19. Evaluation report on the study on the application of 3D woven C/C composite materials; Sanjigenori C/C composite zairyo no kikai buhin eno oyo ni kansuru kenkyu hyoka hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper described a study made during 1995 through 1998 on the application of 3D woven C/C composite materials to machine parts. The materials are large in rigidity, small in specific gravity, excellent in specific rigidity, and suitable for high speed rotation. They are excellent in heat resistance/dynamic reformation and expected as materials which can exceed limits of the existing steel for machine parts. However, to use them as machine parts, a possibility of high precision cutting is required. Therefore, a study was made on metal multi-layer membrane coating to C/C composites. Pitch-system high-elastic carbon fibers were made a 4-directional material by orienting horizontally in 3 directions at 120 degrees per plane and also orienting vertically. Various materials such as W, Mo and WC were coated by the laser assisted plasma hybrid spraying method. High adhesion coats with little pore were obtained. Multi-directionally woven C/C composites used to be poor in surface precision workability, but the problem was able to be solved by coating surfaces with metal layers and metal multi-layer membranes. The application is expanded not only to polygonal mirrors (for laser printing machine) and milling spindles, but largely to machine parts. 11 figs., 1 tab.

  20. Fiscal 1999 survey report. Survey of leading countries' approaches to biomass energy development; 1999 nendo shuyokoku ni okeru biomass energy kaihatsu eno torikumi ni kansuru chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    The survey aims to help Japan deliberate its future biomass energy development strategy including the course Japan is to follow in its research and development of biomass energy by clarifying leading countries' approaches to the subject matter and trends of their research and development efforts in this connection. The states of biomass energy development in the U.S. and Europe are reported. In the U.S., President Clinton issued Executive Order 13134 on August 12, 1999, regarding bio-based products and bioenergy development. In this country, bioenergy and bio-based production technologies have developed to reach a stage where business pays, and commercial plants are in service. The U.S. Administration mentions as a strategy the efficient development of the bioenergy industry. In Europe, where resources are versatile and local, it is difficult to assess the economy of scale, and small-scale development efforts are being accumulated. Practical technologies under development mostly involve direct combustion. European measures are similar to U.S. measures in that such political goals as local development and employment promotion are firmly woven into them. (NEDO)

  1. Měření dohlednosti kamerou pro účely studia vlivu reálné atmosféry na přenos optického bezkabelového spoje

    Czech Academy of Sciences Publication Activity Database

    Brázda, V.; Fišer, Ondřej

    2013-01-01

    Roč. 2013, č. 1 (2013) ISSN 1802-4564 R&D Pro jects: GA ČR(CZ) GAP102/11/1376 Institutional support: RVO:68378289 Keywords : visibility * digital camera * free space optical link Subject RIV: DG - Athmosphere Sciences, Meteorology http://147.228.94.30/index.php?option=com_content&view=article&id=369:meni-dohlednosti-kamerou- pro -uely-studia-vlivu-realne-atmosfery-na-penos-optickeho-bezkabeloveho-spoje&catid=44:islo-12013&Itemid=51

  2. Environmental science, petroleum engineer`s view. CO2 gas reduction technologies for global warming prevention; Sekiyu gijutsusha no shiten kara no kankyo kagaku. Chikyu ondanka eno taio no tame no CO{sub 2} yokusei gijutsu

    Energy Technology Data Exchange (ETDEWEB)

    Nomura, K [Tokyo (Japan)

    1998-08-01

    This paper discusses the techniques concerning diesel engines for cogeneration systems, techniques concerning fuel consumption-saving type lubricating oils and techniques concerning oil refineries, out of the CO2 emission control (energy saving) techniques for coping with the global warming which the petroleum engineers are now tackling. The paper introduces CO2 emission control techniques using a petroleum cogeneration system and studies on NOx removing techniques using catalysts as the techniques concerning diesel engines; techniques for reducing friction loss, which occurs in an engine and a transmission, by improving the recipes of lubricating oils and the development of novel friction modifiers as the fuel consumption-saving type lubricating oil techniques; and the CO2 separating techniques using a system for recovering the low-temperature waste heat produced in petroleum refinement with a high efficiency, or using a solid film such as a molecular sieve as the energy-saving techniques used in oil refineries. 18 refs., 8 figs., 1 tab.

  3. Achievement report on the survey concerning possibility of expansion of ITS technology application to various mobility systems; Kakushu mobility system eno ITS gijutsu tekiyo kakudai kanosei ni kansuru chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The project aims to evaluate the results so far achieved in 'The research and development of ITS (intelligent transportation system) by use of clean energy vehicles' and 'Research and development of ITS technology assisted mobility systems' and the development systems under which the efforts are exerted and to evaluate the possibility of their practical application and the spread of the effects brought about. Ex post facto evaluation is conducted of two types of test systems. The first type includes a rent-a-car for business use in the urban area, a rent-a-car for sightseeing, and a system which is an ITS provided electric car as a domestic second car in the residential district. The second type includes a shuttle bus park-and-ride system and a natural gas fueled physical distribution truck. The result is that the ITS provided electric car system is evaluated high by evaluation committee members. The score lowers in the order of the system for business use in the urban area, the system for sightseeing, and the second car system in the residential district. An electric car reduces its environmental impact when it uses a power generation system that utilizes renewable energy. It has become clear that high convenience will be achieved when a dispatching system is improved for a combination of a 'subminiature electric vehicle and ITS technology' as a method to satisfy the demand for commutation means in case the clientele is one or two people. (NEDO)

  4. Research report for fiscal 1998. Basic research for promoting joint implementation, etc. (conversion of old coal-fired thermoelectric power plants in Poland into combined cycle plants); 1998 nendo chosa hokokusho. Poland sekitan karyoku hatsudensho (kyushiki) combined cycle eno tenkan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    A project is discussed for modernization for energy efficiency enhancement and greenhouse gas reduction. The most effective way to reduce greenhouse gas in Poland is to totally replace the existing coal-fired power plants with natural gas combined cycle plants. Under this project, however, natural gas-fired power generation and integrated coal/brown coal gasification combined cycle power generation are both subjected to study. This is because the power plant modernization project is closely related to the fate of coal/brown coal industries which constitute the important industrial department of Poland. As for the earning rate of the project in case of natural gas-fired combined cycle power generation, the rate will be 13.2% even at the Kaweczyn station which is the highest in earning rate, and this fails to satisfy the project conditions. If integrated coal/brown gasification combined cycle power generation is chosen, the rate will be still lower. When the cost for greenhouse gas reduction is taken up, the Konin station exhibits the lowest of 9 dollars/tCO2, and the others 15-17 dollars/tCO2. When coal gas combined cycle is employed, the cost will be 3-4 times higher. (NEDO)

  5. Fracture mechanical modeling of brittle crack propagation and arrest of steel. 3. Application to duplex-type test; Kozai no zeisei kiretsu denpa teisi no rikigaku model. 3. Konseigata shiken eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Aihara, S.; Tsuchida, Y. [Nippon Steel Corp., Tokyo (Japan); Machida, S.; Yoshinari, H. [The University of Tokyo, Tokyo (Japan). Faculty of Engineering

    1996-12-31

    A proposal was made previously on a model of brittle crack propagation and arrest that considers the effect of crack opening suppression by using unbroken ligaments generated on steel plate surface and the effect that cracks precede in the central part of the plate thickness, based on a local limit stress theory for brittleness fracture. This paper discusses applicability of this model to a mixed type test, and elucidates causes for difference in the arrest tenacity of both types in a double tensile test of the standard size. The brittle crack propagation and arrest model based on the local limit stress theory was found applicable to a simulation of the mixed type test. Experimental crack propagation speed history and behavior of the arrest were reproduced nearly completely by using this model. When load stress is increased, the arrests in the mixed type test may be classified into arrests of both inside the steel plate and near the surface, cracks in the former position or arrest in the latter position, and rush of cracks into both positions. Furthermore, at higher stresses, the propagation speed drops once after cracks rushed into the test plate, but turns to a rise, leading to propagation and piercing. 8 refs., 15 figs., 3 tabs.

  6. Ultimate strength analysis of thin plated structures using eigen-functions. 3rd Report. Application to reliability analysis; Koyu kansu wo mochiita usuita kozobutsu no dansosei kaisekiho. 3. Shinraisei kaiseki eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ueda, Y. [Osaka University, Osaka (Japan). Welding Research Institute; Masaoka, K.; Okada, H. [University of Osaka Prefecture, Osaka (Japan). Faculty of Engineering

    1996-12-31

    A reliability analysis was performed on ultimate strength of a hull by introducing reliability engineerings into the idealized structural unit method. Elements developed under the present study were applied to a model of an actual structure to indicate that even an analysis requiring much time under the finite element method can be performed in a short time and at high accuracy when this method is used. Analysis acted with bending moment and shear force simultaneously was performed on a model used as a structure in experiments carried out by Nishihara, assuming pure bending moment and longitudinal strength during slamming. Then, a reliability analysis was conducted on the same model based on this analysis method to investigate the ultimate strength. In an analysis of an ultimate strength when bending and shearing that assume slamming act upon simultaneously, axial force in the hull side decreases as loading increases, wherein how the shearing force increases can be identified clearly. Although existence of initial bends reduces the strength, the effect of variance in the vicinity of the average value on the reliability is rather small, while the effect due to variance in yield stress is greater. 27 refs., 14 figs., 4 tabs.

  7. Equivalent CTOD concept based on the local approach and its application to fracture performance evaluation of welded joints; Local approach ni motozuku toka CTOD gainen no teian to tsugite hakai seino hyoka eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ohata, M.; Minami, F.; Toyoda, M. [Osaka University, Osaka (Japan). Faculty of Engineering; Tanaka, T.; Arimochi, K. [Sumitomo Metal Industries, Ltd., Osaka (Japan); Glover, A. [Nova Gas Transmission Ltd., Calgary (Canada); North, T. [University of Toronto (Canada)

    1996-12-31

    A proposal was given on an equivalent crack tip opening displacement (CTOD) concept which relates quantitatively fracture performance of a structural member with the result of a three-point bending CTOD test via the Weibull stress based on a local approach. The equivalent CTOD is defined as a CTOD in which a three-point CTOD test piece and a structural member provide the same Weibull stress. Experimental and analytical discussions were performed on X80 steel welded joints. Effectiveness of the equivalent CTOD concept was verified from the fact that the fracture performance in welded joints with a large width estimated from the result of the three-point bending CTOD test using the equivalent CTOD concept showed good correspondence with the fracture performance obtained in the experiments. On the other hand, the result of estimation using the conventional CTOD concept is considerably smaller than measurements. As an application of the equivalent CTOD concept, a new determination procedure was introduced on required fracture tenacity to ensure deformation performance required on structural elements. The required CTOD value shows a trend that the smaller the ratio of yield stress of the welded metals to that in the base material, the greater the required CTOD grows. 16 refs., 19 figs., 3 tabs.

  8. Present situation, current problems and countermeasures to solve them by the material technology group in chemical companies; Kagaku shakai ni okeru sochi zairyo gijutsu no genjo to kadai oyobi sore eno taio

    Energy Technology Data Exchange (ETDEWEB)

    Nakahara, Masao. [Asahi Chemical Industry Corp., Tokyo (Japan). Production Technology Center

    1999-05-15

    A questionnaire investigation about the present situation, current problems and countermeasure to be discussed with by material technology groups in the eight chemical companies was carried out. It has become clear from the analysis of answers obtained that the roll of material group has been changing under the diminishing construction of new plant and the increased needs of stable and sustainable plant operation. The present major subjects of material groups, which are pointed out in the answers, are such new technological problems as the plant life estimation, the dissemination and handing down of material technology and the upbringing of technology successors. The countermeasures to meet those requirements made clear are such technology developments as a new monitoring system, preparation of database on material technology and closer cooperation with relatied associations. It is hoped for that the findings of this study would lead to further and more detailed indentification of common problems to be solved and development of practical countermeasures therefor. (author)

  9. Application of a temperature selective storage tank solar system. Part 4. Fundamental experiment under a solar simulator; Ondo sentaku chikunetsuso no solar system eno tekiyo. 4. Solar simulator ni yoru kiso jikken

    Energy Technology Data Exchange (ETDEWEB)

    Kanayama, K; Endo, N; Baba, H; Okamoto, A [Kitami Institute of Technology, Hokkaido (Japan); Kamiya, Y [Kanto Gakuin University, Yokohama (Japan)

    1996-10-27

    The storage tank is classified into a mixed type and stratified type. The stratified type is judged to be more advantageous from a viewpoint of the effective energy utilization. An experiment was made using a solar simulator to put the system, consisting of a vacuum double-glass tube collector and temperature selective storage tank, to practical use. The ejection position of the storage tank at the top is superior to that at the bottom, in the 60{degree}C layer of three layers (60, 40, and 20{degree}C). The ejection position hardly varies with the shape (straight or elbow) of an ejection port. When the temperature stratified layer is formed in two layers (40 {times} 2, 20{degree}C) to three layers (60, 40, and 20{degree}C), heat can be stably stored as the flow rate is higher. The stratified storage tank is inferior to the mixed storage tank in heat collection efficiency, but the specific exergy increases. By increasing the number of heat storage layers, the result of this experiment can also be applied to the linear temperature gradient layer obtained in the practical use. As a result of the above experiment, the basic data for an automated system design during practical application was obtained. 3 refs., 15 figs.

  10. Application of a temperature selective heat storage tank to a solar system. Part 3. Solar heat collecting system; Ondo sentaku chikunetsuso no solar system eno tekiyo. 3. Shunetsu hoshiki ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Kamiya, Y [Kanto Gakuin University, Yokohama (Japan); Kanayama, K [Kitami Institute of Technology, Hokkaido (Japan)

    1996-10-27

    The tank system and tank-less system when the temperature selective heat storage tank is applied to a solar system were considered. In the tank system, the simulation shows that the annual supplementary heat consumption is reduced as the tank capacity becomes lower. The most suitable operating time set for determining the reference time is about five hours in winter and about nine hours in summer. The annual operating time is about 6.5 hours. In the tank-less system, the most suitable minimum flow rate per solar collector area of a heating medium in Tokyo and its districts is 10 L/hm{sup 2} for both three-step flow control and constant flow control. The tank-less system is slightly lower in annual supplementary heat consumption than the tank system. For the three-step flow control, a change in the annual supplementary heat consumption is lower than that in the minimum flow rate. For the constant flow control, however, the annual supplementary heat consumption rapidly increases when the flow rate more decreases than the optimum value. The number of pump start/stop counts for the three-step flow control is less than two times as high as for the constant flow control. 4 refs., 6 figs., 1 tab.

  11. Separation and purification of lactic acid. Thermal catalytic depolymerization of poly-lactic acid into lactide; Hakkoho nyusan no bunri seisei ni kansuru kenkyu. Pori nyusan no rakuchido eno sesshokuteki netsukai jugo

    Energy Technology Data Exchange (ETDEWEB)

    Morita, M.; Hirama, Y.; Liew, M. [Hokkaido National Industrial Research Institute, Sapporo (Japan)

    1996-05-10

    A new separation and purification method for lactic acid from fermentation broth is proposed by which poly-lactic acid produced from unpurified lactic acid is catalytically depolymerized into lactide fractions then further purified into lactide. In the present study, thermal depolymerization catalysts were investigated for commercial use. Iron catalysts, especially metallic iron, and ferrous oxide and lactate, were found to provide almost the same catalytic activity and lactide composition in depolymerization products and those in tin octoate and antimony oxide catalysts. Ferrous oxide was also applied to depolymerize poly-lactic acid derived form unpurified lactic acid to compare catalytic activity and lactide composition and was confirmed to show results similar to those of pure polymer. Based on these findings, it is concluded that iron catalysts can be used commercially. Furthermore, catalytic depolymerization of poly-lactic acids with different molecular weights were studied. Polymers with Mw 5,000-10,000 were found to be better for production of lactide, based on the behavior of depolymerization and lactide content in the product. 5 refs., 9 figs., 1 tab.

  12. Development of new technologies for high quality thin film and its application to energy engineering; Hikari seigyo to sokudo senbetsu ni yoru chokinshitsu usumaku no seisaku to energy bun`ya eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Hijikata, K; Inoue, T; Nagasaki, T; Suzuki, Y; Sato, I [Tokyo Institute of Technology, Tokyo (Japan); Nakabeppu, O [The University of Tokyo, Tokyo (Japan). Faculty of Engineering

    1997-02-01

    This paper describes laser irradiation and velocity selective deposition experiments for fabricating high quality thin films. For the formation of Ag thin film by vacuum deposition method, YAG laser was irradiated to atoms or clusters parallel or perpendicular to the NaCl single crystal substrate, to deposit them on the substrate. For another experiment, Ag atoms selected in the given velocity were deposited on the (001) NaCl substrate by passing the molecular beam through the velocity selector. When laser was not irradiated, the thin film showed a random structure. Epitaxial growth was accelerated by the laser irradiation. When the selective velocity was set in 353 m/s at the constant intensity of molecular beam, the diffraction pattern of the thin film showed net pattern. When the selective velocity was set in 529 m/s, a structure in which ring pattern was overlapped on the net pattern was obtained. 2 figs.

  13. Development of hydriding alloys with multi-functionally-graded properties and their applications to energy conversion devices; Keishagata fukugo kino wo hyomen ni motsu suiso kyuzo gokin no kaihatsu to energy henkan gijutsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Suda, S; Kadoma, H; Nagamoto, H; Okura, T [Kogakuin University, Tokyo (Japan)

    1997-02-01

    This paper describes the formation of fluoride layer on the surface of hydriding alloys. The fluoride formation reaction consists of a reduction removal process of surface oxide and a fluorination process. Specific surface area of alloy grains can be increased by the hydrogenation reaction in the surface layer accompanied with the removal of surface oxide, which results in easier permeation of molecular hydrogen into fluoride layer. During the fluorination process, a large amount of Ni in the alloy components is eluted, which results in the reduction of Ni distribution immediately under the fluoride layer in the alloy. Consequently, collector sites near the surface are reduced, and conductivity among alloy grains is degraded. To enhance the hydrogen collector sites, specific surface area of alloy grains can be increased by controlling the pH value of fluorination treatment solution in a given range. Moreover, performance of fluoride layer can be advanced by electrochemically dispersing metal Ni in the fluoride layer using Ni complex ion mixed in the treatment solution. 2 refs., 3 figs.

  14. Effect of sail on augmenting attitude stability of hovering VTOL vehicle supported by one-ducted-fan. Ductedter dot fan shiji VTOL ki no hover shisei anteisei eno ho no eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Ando, S.; Kato, M. (Nagoya University, Nagoya (Japan). Faculty of Engineering)

    1991-07-05

    An analytical investigation was given on augmenting attitude stability of a VTOL airplane supported by one-ducted-fan, at hovering, by means of installing a sail having air resistance on a place higher than the vehicle center-of-gravity. Since the problem becomes nonlinear, the time-marching technique was used. The sail on which the emphasis was placed had an area of as less than several times as much of the duct sectional area, and a height of as less than several times as much of the duct diameter. The stabilization effect is expressed as {Delta}h = center of gravity height region providing the stability/duct diameter. Without a sail, {Delta}h was several percent, but with a sail, the value increased to about ten times as much. This increase in {Delta}h was remarkable in the direction that elevate the position of the center of gravity. More stability is obtained by increasing the sail height than by increasing the area. Substitutes to a sail, working on the same principle, could be a gas-bag and wind mill. A gas-bag, which can change its volume readily, will be able to vary the sail area easily. A wind mill could make the weight lighter than a sail, and has a possibility of varying the resistance through changing its blade pitch angle. 2 refs., 2 figs., 1 tab.

  15. Report on FS survey for project of introduction of highly energy-efficient trolley buses into the Mexico Metropolitan Area in FY 1997; 1997 nendo chosa hokokusho (Mexico city eno sho energy gata trolley bus donyu project FS chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    Traffic jam and air pollution by automobiles are currently getting severe in the Mexico Metropolitan Area. Trolley buses were introduced for improving the environment more than ten years ago. Vehicles become too old for use, and their energy efficiency is low. In this project, 200 highly energy-efficient trolley buses are introduced to replace old inefficient trolley buses, which results in the improved traffic convenience in the City and enhanced energy efficiency. The new energy-efficient trolley buses are made of energy-efficient control VVVF inverters produced and exported by MELCO (Mitsubishi Electric Corporation), energy-efficient motors by MELMEX (Mitsubishi Electric Corporation, Mexico), and bodies by MASA which is a major manufacturer of bus bodies. The objective of this research is to analyze the background of introduction of new energy-efficient trolley buses and the effects of the present project. The results of analysis are going to be utilized for future promotion of the Joint Implementation and Clean Development Mechanism. 30 refs., 30 figs., 24 tabs.

  16. Desarrollo y aplicación de índices y simulaciones para la vigilancia y el pronóstico a mediano plazo del impacto del ENOS frente a la costa peruana

    OpenAIRE

    Quispe Ccalluari, Carlos; Tam Málaga, Jorge; Arrellano Rojas, Cinthia; Chamorro Gómez, Adolfo; Espinoza Morriberón, Dante; Romero Tadeo, Carlos; Ramos Flores, Jorge

    2017-01-01

    El propósito del trabajo es presentar índices oceanográficos del Pacifico Sudeste, pronósticos de la propagación de las ondas Kelvin ecuatoriales (OKE) y pronósticos del Índice Térmico Costero Peruano (ITCP), usando como un ejemplo la información observacional actualizada hasta setiembre 2016. Durante el trimestre julio-agosto-setiembre (JAS) 2016, los Índices Térmico Costero Peruano (ITCP) y del Anticiclón del Pacífico Sur (IAPS) presentaron condiciones neutras, el Índice del Áre...

  17. Preparation of Bi-Pb-Sr-Ca-Cu-O high Tc thick films on Ag or MgO substrate with superconductor paste; Bi kei ko Tc chodendo paste ni yoru Ag, MgO kibanjo eno atsumaku sakusei

    Energy Technology Data Exchange (ETDEWEB)

    Takabatake, N. [Ishikawa Technical High School, Ishikawa (Japan); Tsubota, T.; Ishikawa, T.; Ohashi, K. [Kanazawa Institute of Technology, Ishikawa (Japan)

    1995-07-15

    The following were reported on making Bi series superconducting thick films by a wet method. A paste was made by adding PSO (or ethanol solution of ethylenegrycol) of Yushiro Chemical Industry Co., Ltd. as a binder to a Bi series 2223 single-phase powder sample (blending composition Bi:Pb:Sr:Ca:Cu=1.8:0.4:2:2:3.2); the sample was then coated on an Ag (or MgO) substrate with a brush; after being dried at 105{degree}C for one hour, it was thermally decomposed at 700{degree}C for one hour; then, the process of prissurized forming (at pressure 2 to 5 ton f/cm{sup 2}) and of sintering at 845{degree}C for 20 hours or more were performed on the sample to form a thick film sample. A critical temperature Tc, critical current density Jc, etc., were measured on such thick film sample. As a result, nearly same values were obtained as Tc (93K) and Jc (84A/cm{sup 2}) which were those of a bulk sample made by using the same powder sample, pressurizing at 2 ton f/cm{sup 2} for pelletizing, and sintering at 845{degree}C for 20 hours. 6 refs., 7 figs., 1 tab.

  18. Příroda a domov : postřehy z pouště: domov je něco, co je na přírodě vyvzdorováno a odlišeno

    Czech Academy of Sciences Publication Activity Database

    Cílek, Václav

    2010-01-01

    Roč. 21, č. 23 (2010), s. 79-79 ISSN 0862-6545 Institutional research plan: CEZ:AV0Z30130516 Keywords : nature * desert * desert animals * nature a culture * man and nature Subject RIV: AC - Archeology, Anthropology, Ethnology

  19. enos tradičních hodnot Romů ve smíšených manželstvích romsko-českých

    OpenAIRE

    Innemanová, Petra

    2007-01-01

    The goal of my graduation treatise is research of three generations of mixed Romany-Czech marriages in the aspects of upbringing, education and traditions and how and how many of these aspects were transmitted in a progress of time. Which Romanian traditional values have kept during the theree genarations of the inter marriages. In the theoretical part of my treatise I tried to describe all topics which I then researched in the practical part with individual couples which were namely the fell...

  20. Aspiration toward geothermal energy utilization in regional development plan. Part 6. ; Hydrothermal fluid utilization business in Matsuo-mura of Iwate prefecture. Chiiki keikaku ni okeru 'chinetsu riyo' eno hofu. 6. ; Iwateken Matsuomura no chinetsu nessui riyo jigyo

    Energy Technology Data Exchange (ETDEWEB)

    Otobe, Y; Furutate, E

    1992-10-31

    Twenty six years have passed since the first geothermal power station was constructed in Matsuo-mura of Iwate Prefecture, Japan. This paper describes the history, the present situation and the future conception of the geothermal energy utilization in this village. This village includes Hachimantai of a vantage ground in the center and has the gross area of 233.8km[sup 2], the annual average temperature of 8.3 centigrade and the continuous snow cover period of about 100 days. The hot water leading facility was cooperatively constructed by Japan Metals and Chemicals, Hachimantai Hot Spring Development and Matsuo-mura. The total working expense is 539.3 million yen. Hot water sources are the condensate from the condenser of geothermal power plant and hot spring. This mixed hot water of 4.3 t/min is led to respective facilities. The hot water supplying channel has the length of 12.8km from the power station through the Hachimantai hot spring resort, Kamiyogi to Takaishino. Respective total areas of greenhouses using hydrothermal fluid in both districts are 1,075ha and the inlet temperature of hot water is 60 centigrade and kinds of crop are 5 like green pepper and others. Takaishino agricultural park has selected flower and ornamental plant culture such as poppy anemone, stock and statice which are suitable for this district of low temperature and insufficient sunshine. The planted area is 10,700m[sup 2]. 2 refs., 9 figs., 4 tabs.

  1. Bi-axial M-. Phi. analyses of RC columns using fiber model and comparison with experimental results. 2 jikumage wo ukeru hashirabuzai no M-. Phi. kankei eno fiber model no tekigosei

    Energy Technology Data Exchange (ETDEWEB)

    Murayama, Y.; Tokuyama, S.; Furuichi, K. (Kajima Corp., Tokyo (Japan))

    1991-10-31

    In order to examine the accuracy of a fiber model for analyzing the stability of towers of a cable-stayed bridge subjected to biaxial bending force due to earthquake, experimental results of column test specimens were compared with analytical results. The relation between biaxial bending moment and curvature (M-{Phi}) was measured in biaxial bending model experiment using a reinforcement ratio, axial compressive force and loading pattern as parameters. Since the relation was greatly affected by axial modeling of a reinforcing material, the parameter analysis was conducted by paying particular attention to reinforcement models (bilinear model, cubic one and Ramberg-Osgood one). As a result, the Ramberg-Osgood model was suitable for columns with a large longitudinal reinforcement ratio and large axial tension such as seen in highrise buildings, while the cubic model was more suitable for columns with a small ratio such as seen in cable-stayed bridges. 4 refs., 10 figs., 1 tab.

  2. Energy-based damping evaluation of cable-stayed bridges and its application to Tsurumi Tsubasa bridge; Shachokyo shindo gensui no energy teki hyokaho to Tsurumi Tsubasakyo eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, H. [Saitama University, Saitama (Japan). Faculty of Engineering; Takano, H.; Ogasawara, M.; Shimosato, T. [Metropolitan Expressway Public Corp., Tokyo (Japan); Kato, M.; Kato, H. [NKK Corp., Tokyo (Japan)

    1996-07-21

    This paper provides and discusses a more common energy-based evaluation method of vibration damping in cable-stayed bridges. This method was applied to data obtained from the field vibration test of the Tsurumi Tsubasa Bridge. The damping was defined as dissipation energy in one cycle against the total potential energy. The dissipation energy from shoe friction, aerodynamic damping, and dampers for cables was added to the method proposed by Yamaguchi, et. al., in which the energy of girders, towers, and cables were quantitated, and the dissipation energy was determined from the loss factor of each constituent, to evaluate the damping of whole bridge. Thus, a more common energy-based evaluation method was provided. This method was applied to the damping obtained from the field vibration test of Tsurumi Tsubasa Bridge. Consequently, it was found that the damping of whole bridge was significantly affected by the shoe friction, aerodynamic damping, and dampers for cables. Distinguished damping characteristics of the Tsurumi Tsubasa Bridge could be explained by the energy-based evaluation. Validity of the energy-based evaluation method for damping of cable-stayed bridges was demonstrated. 15 refs., 9 figs., 6 tabs.

  3. Fracture mechanical modeling of brittle crack propagation and arrest of steel. 3. Application to duplex-type test; Kozai no zeisei kiretsu denpa teisi no rikigaku model. 3. Konseigata shiken eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Aihara, S; Tsuchida, Y [Nippon Steel Corp., Tokyo (Japan); Machida, S; Yoshinari, H [The University of Tokyo, Tokyo (Japan). Faculty of Engineering

    1997-12-31

    A proposal was made previously on a model of brittle crack propagation and arrest that considers the effect of crack opening suppression by using unbroken ligaments generated on steel plate surface and the effect that cracks precede in the central part of the plate thickness, based on a local limit stress theory for brittleness fracture. This paper discusses applicability of this model to a mixed type test, and elucidates causes for difference in the arrest tenacity of both types in a double tensile test of the standard size. The brittle crack propagation and arrest model based on the local limit stress theory was found applicable to a simulation of the mixed type test. Experimental crack propagation speed history and behavior of the arrest were reproduced nearly completely by using this model. When load stress is increased, the arrests in the mixed type test may be classified into arrests of both inside the steel plate and near the surface, cracks in the former position or arrest in the latter position, and rush of cracks into both positions. Furthermore, at higher stresses, the propagation speed drops once after cracks rushed into the test plate, but turns to a rise, leading to propagation and piercing. 8 refs., 15 figs., 3 tabs.

  4. Equivalent CTOD concept based on the local approach and its application to fracture performance evaluation of welded joints; Local approach ni motozuku toka CTOD gainen no teian to tsugite hakai seino hyoka eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ohata, M; Minami, F; Toyoda, M [Osaka University, Osaka (Japan). Faculty of Engineering; Tanaka, T; Arimochi, K [Sumitomo Metal Industries, Ltd., Osaka (Japan); Glover, A [Nova Gas Transmission Ltd., Calgary (Canada); North, T [University of Toronto (Canada)

    1997-12-31

    A proposal was given on an equivalent crack tip opening displacement (CTOD) concept which relates quantitatively fracture performance of a structural member with the result of a three-point bending CTOD test via the Weibull stress based on a local approach. The equivalent CTOD is defined as a CTOD in which a three-point CTOD test piece and a structural member provide the same Weibull stress. Experimental and analytical discussions were performed on X80 steel welded joints. Effectiveness of the equivalent CTOD concept was verified from the fact that the fracture performance in welded joints with a large width estimated from the result of the three-point bending CTOD test using the equivalent CTOD concept showed good correspondence with the fracture performance obtained in the experiments. On the other hand, the result of estimation using the conventional CTOD concept is considerably smaller than measurements. As an application of the equivalent CTOD concept, a new determination procedure was introduced on required fracture tenacity to ensure deformation performance required on structural elements. The required CTOD value shows a trend that the smaller the ratio of yield stress of the welded metals to that in the base material, the greater the required CTOD grows. 16 refs., 19 figs., 3 tabs.

  5. Ultimate strength analysis of thin plated structures using eigen-functions. 3rd Report. Application to reliability analysis; Koyu kansu wo mochiita usuita kozobutsu no dansosei kaisekiho. 3. Shinraisei kaiseki eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ueda, Y [Osaka University, Osaka (Japan). Welding Research Institute; Masaoka, K; Okada, H [University of Osaka Prefecture, Osaka (Japan). Faculty of Engineering

    1997-12-31

    A reliability analysis was performed on ultimate strength of a hull by introducing reliability engineerings into the idealized structural unit method. Elements developed under the present study were applied to a model of an actual structure to indicate that even an analysis requiring much time under the finite element method can be performed in a short time and at high accuracy when this method is used. Analysis acted with bending moment and shear force simultaneously was performed on a model used as a structure in experiments carried out by Nishihara, assuming pure bending moment and longitudinal strength during slamming. Then, a reliability analysis was conducted on the same model based on this analysis method to investigate the ultimate strength. In an analysis of an ultimate strength when bending and shearing that assume slamming act upon simultaneously, axial force in the hull side decreases as loading increases, wherein how the shearing force increases can be identified clearly. Although existence of initial bends reduces the strength, the effect of variance in the vicinity of the average value on the reliability is rather small, while the effect due to variance in yield stress is greater. 27 refs., 14 figs., 4 tabs.

  6. Fiscal 1997 report on the feasibility survey of Japan`s cooperation into energy/environment related fields in Asian countries; 1997 nendo chosa hokokusho (Asia shokoku ni okeru energy kankyo kanren bun`ya eno kyoryoku kanosei chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    A survey was made to study possibilities of Japan`s cooperation in tackling energy/environment problems in Asian countries. In 1995 the GNP growth rate was 2.6% in the world, while it was 7.9% in Asia showing a rapid growth. With the accelerating industrialization and urbanization, energy demand is now over three times as much as that in the 1980s. This means increases in waste and environmental pollution. Especially, the use of firewood in non-electrified areas is a serious problem the same as the slash-and-burn farming and deforestation. Also relating to the production of electric power, it is inevitable to choose hydroelectric power and renewable energy in addition to fossil fuels. Therefore, the necessity is heightening of various technologies for it and environmental protection technologies such as coal cleaning. Local areas, where the population density is extremely low and the electrification cost is high, became victims of elecrification. There the power system should be shifted from the central supply system to the local distributed one. For it, it is necessary to recognize an importance of the distributed type renewable energy which is friendly to the environment. The paper outlined the state of each country and energy by policy and environment. 10 figs., 83 tabs.

  7. Synthesis and crytallization of amorphous In-Te alloys

    International Nuclear Information System (INIS)

    Vengrenovich, R.D.; Lopatnyuk, I.A.; Mikhal'chenko, V.P.; Kasiyan, I.M.; Geshko, E.I.

    1988-01-01

    Tendency of Te-In alloys with indium content from 5 to 40 % to amorphization is investigated. It is marked that in this interval of concentrations the alloys have the tendency to subcooling even at cooling velocities equalling only 0.2-0.3 K/s. Maximal subcooling ΔT=70 deg takes place for the eutectic composition. Tendency of Te-In alloys to vitrification is explained by the character to interatomic interactions in a liquid, the interactions promote the formation of molecular clusters in it in cooling, that leads to fast increase of viscosity and to increase of T g amorphization temperature

  8. Resveratrol and Endothelial Nitric Oxide

    Directory of Open Access Journals (Sweden)

    Ning Xia

    2014-10-01

    Full Text Available Nitric oxide (NO derived from the endothelial NO synthase (eNOS has antihypertensive, antithrombotic, anti-atherosclerotic and antiobesogenic properties. Resveratrol is a polyphenol phytoalexin with multiple cardiovascular and metabolic effects. Part of the beneficial effects of resveratrol are mediated by eNOS. Resveratrol stimulates NO production from eNOS by a number of mechanisms, including upregulation of eNOS expression, stimulation of eNOS enzymatic activity and reversal of eNOS uncoupling. In addition, by reducing oxidative stress, resveratrol prevents oxidative NO inactivation by superoxide thereby enhancing NO bioavailability. Molecular pathways underlying these effects of resveratrol involve SIRT1, AMPK, Nrf2 and estrogen receptors.

  9. On the application of subcell resolution to conservation laws with stiff source terms

    International Nuclear Information System (INIS)

    Chang, S.

    1989-11-01

    LeVeque and Yee recently investigated a one-dimensional scalar conservation law with stiff source terms modeling the reacting flow problems and discovered that for the very stiff case most of the current finite difference methods developed for non-reacting flows would produce wrong solutions when there is a propagating discontinuity. A numerical scheme, essentially nonoscillatory/subcell resolution - characteristic direction (ENO/SRCD), is proposed for solving conservation laws with stiff source terms. This scheme is a modification of Harten's ENO scheme with subcell resolution, ENO/SR. The locations of the discontinuities and the characteristic directions are essential in the design. Strang's time-splitting method is used and time evolutions are done by advancing along the characteristics. Numerical experiment using this scheme shows excellent results on the model problem of LeVeque and Yee. Comparisons of the results of ENO, ENO/SR, and ENO/SRCD are also presented

  10. Exhaled Nitric Oxide is Decreased by Exposure to the Hyperbaric Oxygen Therapy Environment

    Directory of Open Access Journals (Sweden)

    Zudin A. Puthucheary

    2006-01-01

    or 40% oxygen, 1 ATA. In an in vitro study, nitrate/nitrite release decreased after 90 minutes HBOT in airway epithelial (A549 cells. Conclusion. HBO exposure causes a fall in eNO. Inducible nitric oxide synthase (iNOS may cause elevated eNO in patients secondary to inflammation, and inhibition of iNOS may be the mechanism of the reduction of eNO seen with HBOT.

  11. Exhaled Nitric Oxide Is Useful in Symptomatic Radioactive Pneumonia: A Retrospective Study

    Directory of Open Access Journals (Sweden)

    Jiancheng Li

    2017-01-01

    Full Text Available The aim was to defect the exhaled nitric oxide (eNO prediction value of symptomatic radioactive pneumonia (SRP. 64 cases of lung cancer or esophagus cancer, who had the primary radiotherapy (intensity-modulated radiation therapy, were included from 2015 June to 2016 January. During the following, the patients were divided: the symptomatic radiation pneumonia group (SRP, with the CTCAE v4.0 score > 2 and the asymptomatic radiation pneumonia group (ASRP, with CTCAE v4.0 score ≤ 1. All the patients were measured eNO before and at the end of thoracic radiotherapy and gain the posttherapy eNO value and the eNO ratio (posttherapy eNO value/pretherapy eNO value, then the predictive values of eNO toward SRP were measured using the receiver-operating characteristic (ROC. 17 cases were included in the SRP group and the other 47 were included in the ASRP group. The posttherapy eNO was 29.35 (19~60 bbp versus 20.646 (11~37 (P<0.001, and the ratio was 1.669 (0.61~3.5 versus 0.920 (0.35~1.5 (P<0.01 (symptomatic versus asymptomatic. ROC showed that the cutoff value of SRP was 19.5 bbp (posttherapy eNO, area under concentration-time curve (AUC = 0.879 and 1.305 (eNO ratio, AUC = 0.774, which meant that posttherapy eNO and eNO ratio were useful in finding SRP.

  12. Association of endothelial nitric oxide synthase gene polymorphism with the risk of Henoch-Schönlein purpura/Henoch-Schönlein purpura nephritis.

    Science.gov (United States)

    Zhong, Weiqiang; Zhou, Tian-Biao; Jiang, Zongpei

    2015-04-01

    Association between endothelial nitric oxide synthase (eNOS) gene polymorphism and Henoch-Schönlein purpura (HSP)/Henoch-Schönlein purpura nephritis (HSPN) risk is still controversial. A meta-analysis was performed to evaluate the association between eNOS gene polymorphism and HSP/HSPN susceptibility. A predefined literature search and selection of eligible relevant studies were performed to collect data from electronic database. Three articles were identified for the analysis of association between eNOS gene polymorphism and HSPN/HSP risk. eNOS G894T gene polymorphism was not associated with HSPN susceptibility and the risk of patients with HSP developing into HSPN. Interestingly, eNOS G894T T allele and GG genotype were associated with HSP susceptibility, but not the TT genotype. eNOS T786C TT genotype was associated with HSPN susceptibility, but not C allele and CC genotype. Furthermore, eNOS T786C gene polymorphism was not associated with HSP risk and the risk of patients with HSP developing into HSPN. In conclusion, eNOS T786C TT genotype was associated with and eNOS G894T T allele and GG genotype were associated with HSP susceptibility. However, more studies should be performed in the future.

  13. Posttranslational inactivation of endothelial nitric oxide synthase in the transgenic sickle cell mouse penis

    Science.gov (United States)

    Musicki, Biljana; Champion, Hunter C.; Hsu, Lewis L.; Bivalacqua, Trinity J.; Burnett, Arthur L.

    2017-01-01

    INTRODUCTION Sickle cell disease (SCD)-associated priapism is characterized by endothelial nitric oxide synthase (eNOS) dysfunction in the penis. However, the mechanism of decreased eNOS function/activation in the penis in association with SCD is not known. AIMS Our hypothesis in the present study was that eNOS is functionally inactivated in the SCD penis in association with impairments in eNOS posttranslational phosphorylation and the enzyme’s interactions with its regulatory proteins. METHODS Sickle cell transgenic (sickle) mice were used as an animal model of SCD. Wild type (WT) mice served as controls. Penes were excised at baseline for molecular studies. eNOS phosphorylation on Ser-1177 (positive regulatory site) and Thr-495 (negative regulatory site), total eNOS, and phosphorylated AKT (upstream mediator of eNOS phosphorylation on Ser-1177) expressions, and eNOS interactions with heat shock protein 90 (HSP90) and caveolin-1 were measured by Western blot. Constitutive NOS catalytic activity was measured by conversion of L-[14C]arginine-to-L-[14C]citrulline in the presence of calcium. MAIN OUTCOME MEASURES Molecular mechanisms of eNOS dysfunction in the sickle mouse penis. RESULTS eNOS phosphorylated on Ser-1177, an active portion of eNOS, was decreased in the sickle mouse penis compared to WT penis. eNOS interaction with its positive protein regulator HSP90, but not with its negative protein regulator caveolin-1, and phosphorylated AKT expression, as well as constitutive NOS activity, were also decreased in the sickle mouse penis compared to WT penis. eNOS phosphorylated on Thr-495, total eNOS, HSP90, and caveolin-1 protein expressions in the penis were not affected by SCD. CONCLUSION These findings provide a molecular basis for chronically reduced eNOS function in the penis by SCD, which involves decreased eNOS phosphorylation on Ser-1177 and decreased eNOS-HSP90 interaction. PMID:21143412

  14. Requirement of phosphorylatable endothelial nitric oxide synthase at Ser-1177 for vasoinhibin-mediated inhibition of endothelial cell migration and proliferation in vitro.

    Science.gov (United States)

    García, Celina; Nuñez-Anita, Rosa Elvira; Thebault, Stéphanie; Arredondo Zamarripa, David; Jeziorsky, Michael C; Martínez de la Escalera, Gonzalo; Clapp, Carmen

    2014-03-01

    Endothelial nitric oxide synthase (eNOS)-derived nitric oxide is a major vasorelaxing factor and a mediator of vasopermeability and angiogenesis. Vasoinhibins, a family of antiangiogenic prolactin fragments that include 16 K prolactin, block most eNOS-mediated vascular effects. Vasoinhibins activate protein phosphatase 2A, causing eNOS inactivation through dephosphorylation of eNOS at serine residue 1179 in bovine endothelial cells and thereby blocking vascular permeability. In this study, we examined whether human eNOS phosphorylation at S1177 (analogous to bovine S1179) influences other actions of vasoinhibins. Bovine umbilical vein endothelial cells were stably transfected with human wild-type eNOS (WT) or with phospho-mimetic (S1177D) or non-phosphorylatable (S1177A) eNOS mutants. Vasoinhibins inhibited the increases in eNOS activity, migration, and proliferation following the overexpression of WT eNOS but did not affect these responses in cells expressing S1177D and S1177A eNOS mutants. We conclude that eNOS inhibition by dephosphorylation of S1177 is fundamental for the inhibition of endothelial cell migration and proliferation by vasoinhibins.

  15. Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells.

    Science.gov (United States)

    Ugusman, Azizah; Zakaria, Zaiton; Hui, Chua Kien; Nordin, Nor Anita Megat Mohd

    2010-07-01

    Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs). HUVECS WERE DIVIDED INTO FOUR GROUPS: control, treatment with 180 microM hydrogen peroxide (H(2)O(2)), treatment with 150 microg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H(2)O(2) for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction. Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs. Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs.

  16. Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells

    Directory of Open Access Journals (Sweden)

    Azizah Ugusman

    2010-01-01

    Full Text Available OBJECTIVE: Nitric oxide produced by endothelial nitric oxide synthase (eNOS possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs. METHODS: HUVECs were divided into four groups: control, treatment with 180 μM hydrogen peroxide (H2O2, treatment with 150 μg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction. RESULTS: Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs. CONCLUSION: Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs.

  17. Six Open Questions about the Migration of Engineered Nano-Objects from Polymer-Based Food Contact Materials: A Review

    DEFF Research Database (Denmark)

    Jokar, Maryam; Pedersen, Gitte Alsing; Löschner, Katrin

    2017-01-01

    . Current research focusing on FCMs has often reached inconsistency regarding migration of ENOs. The scope of this critical review is to give a concise overview of the most relevant aspects of the subject, and to identify and discuss the major open questions in relation to migration of ENOs from FCMs....... This includes the very fundamental questions whether ENOs can migrate from FCMs at all and what the potential release mechanisms of ENOs could be. The inconsistency of findings from experimental studies is highlighted based on the example of silver nanoparticle migration from polymer-based FCMs. Challenges...

  18. Crystal structure of enolase from Drosophila melanogaster.

    Science.gov (United States)

    Sun, Congcong; Xu, Baokui; Liu, Xueyan; Zhang, Zhen; Su, Zhongliang

    2017-04-01

    Enolase is an important enzyme in glycolysis and various biological processes. Its dysfunction is closely associated with diseases. Here, the enolase from Drosophila melanogaster (DmENO) was purified and crystallized. A crystal of DmENO diffracted to 2.0 Å resolution and belonged to space group R32. The structure was solved by molecular replacement. Like most enolases, DmENO forms a homodimer with conserved residues in the dimer interface. DmENO possesses an open conformation in this structure and contains conserved elements for catalytic activity. This work provides a structural basis for further functional and evolutionary studies of enolase.

  19. Araabia šeik ostis tüki Tallinnas tegutsevast pangast / Mikk Salu

    Index Scriptorium Estoniae

    Salu, Mikk, 1975-

    2006-01-01

    SBM panga omaniku Marfin Grupi suurimaks aktsionäriks tõusis Dubai Financials, mis on autori sõnul emiiriperekonna Al Maktoumi võimalus Euroopasse sisenemisel. Marfini peajuristi Fotios Karatzenise hinnangul on huvitavad investeerimisobjektid Eestis sadamad ja kinnisvara. Vt. samas intervjuud Fotios Karatzenisega. Lisad: Teine tase; Marfin Grupp: Kreeka-Araabia pank

  20. NG Kunstikonteiner alustab kirjandusega

    Index Scriptorium Estoniae

    2008-01-01

    Kunstnik Sandra Jõgeva korraldab Tallinnas kultuuritehases Polymer alternatiivgaleriis Non Grata Kunstikonteiner kirjanduslike kohtumiste seeria "Üks hullem kui teine". Kirjandusüritused ei ole mõeldud ainult oma ala insider'itele. 17. juulil esinevad Armin Kõomägi teemal "Kirjandus ja äri", Andrus Elbing "Vangla sinus eneses" jt. Vt. ka Looming, 2008, nr. 8, lk. 1271

  1. Genetic diversity in barley landraces (Hordeum vulgare L. subsp ...

    Indian Academy of Sciences (India)

    SDS-PAGE was performed to separate the extracted pro- teins by using 12% separating and 5% stacking gels with a. Mini Protean System from Bio-Rad according to Laemmli modified method (Laemmli 1970). In this study, we adopted constant current electrophoresis instead of constant voltage. The loading of sample was ...

  2. Ansip : uus valitsus tuleb sel nädalal / Anneli Ammas, Jaanus Piirsalu

    Index Scriptorium Estoniae

    Ammas, Anneli, 1962-

    2005-01-01

    Reformierakonna esimees Andrus Ansip on seisukohal, et kokkulepped valitsuse moodustamiseks sõlmitakse sel nädalal, Riigikogu juhatuse valimised näitasid, et ratsionaalseteks kokkulepeteks ollakse valmis. Peale A. Ansipi on teine võimalik peaministrikandidaat Villu Reiljan. Lisa: Erakondade positsioonid kõnelustel

  3. Tõhus ja õiglane menetlus õigusakti põhiseadusvastasuse nõude lahendamisel / Tim Kolk

    Index Scriptorium Estoniae

    Kolk, Tim

    2012-01-01

    Põhiseaduse § 15 lõike 1 teine lause sätestab igaühe õiguse nõuda oma kohtuasja läbivaatamisel mis tahes asjassepuutuva seaduse, muu õigusakti või toimingu põhiseadusvastaseks tunnistamist. Kohustustest, mis see säte paneb kohtunikele ning kuidas erinevates kohtumenetlustes seda õigust kasutada ja kohustusi täita

  4. Lapimaal alla kukkunud kopterit juhtis Eesti piirivalve piloot / Küllike Rooväli

    Index Scriptorium Estoniae

    Rooväli, Küllike, 1969-

    2005-01-01

    21. septembril Soomes Lapimaal Vuotso küla lähedal alla kukkunud väikekopterit Schweizer 300C juhtis eestlane - piirivalve lennusalga päästekopteri teine piloot Rannel Vassiljev. Lisa, kaart: Kopteriõnnetus Lapimaal

  5. Neighbors uneasy about radioactive waste proposal / Milda Seputyte

    Index Scriptorium Estoniae

    Seputyte, Milda

    2005-01-01

    Lätlased on vastu Leedu plaanile rajada Läti-Leedu piiri lähedusse lühiajaliste radioaktiivsete jäätmete ladustuskoht. Läti keskkonnaminister Raimonds Vejonis saatis oma Leedu kolleegile kirja, milles soovitab leida ettevõtmisele teine koht, kuna lähedal asub Läti jaoks strateegiliselt tähtis turismipiirkond

  6. Romaanid võistu rebimas - August Jakobsonist Sass Hennoni / Jaak Urmet

    Index Scriptorium Estoniae

    Urmet, Jaak, 1979-

    2005-01-01

    2004. a. romaanivõistluse esikoharomaan on Sass Henno "Mina olin siin", teine koht: Olavi Ruitlane "Kaval-Peeter ja Vene-Pagan", kolmas koht: Meelis Friedenthal "Kuldne aeg". Sisaldab võitjate nimekirja 1979-2004 ja andmeid noorte silmapaistvate autorite kohta: Kaarel Kressa, Toomas Verrev, Priit Kruus, Rolf Liiv, André Trinity, Jana Lepik,

  7. II Läänemere maade muinsuskaitsefoorum

    Index Scriptorium Estoniae

    2006-01-01

    Teine Läänemeremaade muinsuskaitsefoorum teemal "Urban Heritage - Collective Privilege" Helsingis 7.-11. VI 2005. Põhikonverentsi jutatasid sisse Riita Nikula, Herb Stovel ja Hans Jacob Roald, kes esitasid oma nägemuse ajaloolise linnakeskonna võimalustest ja ohtudest

  8. Yeast Interacting Proteins Database: YOR302W, YOR047C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available rol of glucose-regulated gene expression; interacts with protein kinase Snf1p, glucose sensors Snf3p and Rgt...tein kinase Snf1p, glucose sensors Snf3p and Rgt2p, and TATA-binding protein Spt1

  9. Sinimägede sõdurid kirjutasid kargeid armastuskirju / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2006-01-01

    6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures 2006

  10. Mai alguses linastub dokfilm "Sinimäed" / Andris Feldmanis

    Index Scriptorium Estoniae

    Feldmanis, Andris, 1982-

    2006-01-01

    Valminud on 6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures

  11. Teise maailmasõja lõpptulemus seisuga 8. mai 2006 - eestlaste võit!

    Index Scriptorium Estoniae

    2006-01-01

    8. mail esilinastub kinos ja õhtul ETV-s 6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures

  12. Eesti võimalus suures sõjas, veel tänagi / Erkki Bahovski

    Index Scriptorium Estoniae

    Bahovski, Erkki, 1970-

    2006-01-01

    9. mail esilinastus ETV-s 6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures 2006

  13. Miljoneid maksev filmisari jõudis teise esilinastuseni / Andri Maimets

    Index Scriptorium Estoniae

    Maimets, Andri, 1979-

    2006-01-01

    6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures 2006

  14. ETV tähistab tantsupäeva kolme filmiga

    Index Scriptorium Estoniae

    2006-01-01

    Rahvusvahelise tantsupäeva puhul 29. apr. on Eesti Televisiooni kavas kolm tantsuprogrammi - Uwe Scholzi tantsulavastus "Suur Missa", tantsufilm "Teine" (filmi režissöör René Vilbre) ja kolmas film on Inglismaal valminud Lloyd Newsoni "Elu hind"

  15. Sunduk kapitana Deppa / Maria Tsheskis

    Index Scriptorium Estoniae

    Tsheskis, Maria

    2006-01-01

    Gore Verbinski lavastatud piraadi-triloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" ("Pirates of the Caribbean : Dead Man's Chest") : Ameerika Ühendriigid 2006. Ülevaatlikult ka esimese filmiga seonduvast. Augustis lõppevad kolmanda filmi võtted

  16. Garri Pottera obideli

    Index Scriptorium Estoniae

    2002-01-01

    J.K. Rowling'u Harry Potteri lugude teine film "Harry Potter ja saladuste kamber" ("Harry Potter and the Chamber of Secrets") : režissöör Chris Columbus ja filmi kassatulu võrdlus teiste suurfilmidega esilinastuse nädalal USAs, Itaalias ja Suurbritannias

  17. A novel TSC2 mutation in a Chinese family with tuberous sclerosis ...

    Indian Academy of Sciences (India)

    1Department of Medical Genetics, College of Basic Medical Science and 2Undergraduate Student Brigade, ... Recent studies in the TSC patients indicated that mutations ... Because of the low back pain he ... tein of 946 amino acids instead of a normal tuberin protein ... In the TSC cohort, more than 90% had skin lesions,.

  18. DNA Vaccines

    Indian Academy of Sciences (India)

    diseases. Keywords. DNA vaccine, immune response, antibodies, infectious diseases. GENERAL .... tein vaccines require expensive virus/protein purification tech- niques as ... sphere continue to remain major health hazards in developing nations. ... significance since it can be produced at a very low cost and can be stored ...

  19. c Intes compo on die stine h osition ets wit istolog of Nile th both gy ...

    African Journals Online (AJOL)

    SAM

    plicates. Fish estigated. Tr had six rep ermined. Th hey were s the most e tein was e .... recommends the use of up to 20% sunflower in the diet of ... to improve the quality of feed. .... Trial 1 was run for a period of 120 days in a pond with a water.

  20. Influence of Substrate Composition on vitro Oxygen Consumption of ...

    African Journals Online (AJOL)

    1974-09-11

    Sep 11, 1974 ... and it activates the angiotensin sys- tem by converting angiotensin I to angiotensin n.l3 It also participates in the de 110\\10 synthesis of fatty acids," pro- teins" and of phospholipids (surfactant)." The oxygen consumed by the lung is used not only for its own basal metabolic needs but for additional metabolic.

  1. Hefty European dough for Lithuanian media bends journalism's core / Linas Jegelevicius

    Index Scriptorium Estoniae

    Jegelevicius, Linas

    2011-01-01

    Euroopa Liidu projekt, mille käigus finantseeritakse kohaliku ajakirjanduse väljaandmist aastatel 2007-2013 ei pruugi olla ajakirjaduseetikaga kooskõlas. 2009. aastal läbiviidud uuringust selgus, et iga teine Leedu elanik leiab, et kättesaadavaim EL-ile toetust avaldav infoallikas on kohalik ajakirjandus

  2. Provintsialnõje strasti... provintsialnõi terror... / Boris Tuch

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2007-01-01

    H. Toompere jun. lavastusest "Sortsid" F. Dostojevski romaani järgi, esietendus 14. veebr. Eesti Draamateatris ja M. Tsumatshenko "Onu unenägu" F. Dostojevski jutustuse järgi, esietendus 15. veebr. Vene Teatris. Artikli teine osa ilmus 9. märts, lk. 25 : ill

  3. Võõrvõimu tekitatud kahju heastamise kriteeriumid : Euroopa Inimõiguste Kohtu praktika / Maris Kuurberg

    Index Scriptorium Estoniae

    Kuurberg, Maris

    2010-01-01

    Euroopa Inimõiguste Kohtu praktikast kohtuasjades, mis puudutavad Euroopa inimõiguste ja põhivabaduste kaitse konventsiooni osalisriigi kohustusi kahju heastamisel, mille on tekitanud enne konventsiooni jõustumist selles riigis riiki okupeerinud või annekteerinud teine riik

  4. Salatoimikud : ma tahan uskuda / Mart Rummo

    Index Scriptorium Estoniae

    Rummo, Mart

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  5. Uuesti avatud salatoimikud / Timo Diener

    Index Scriptorium Estoniae

    Diener, Timo

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  6. Täiesti salatised toimikud nostalgiakastmes / Mart Niineste

    Index Scriptorium Estoniae

    Niineste, Mart, 1983-

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  7. Scully : võin ka lolli mängida / Gillian Anderson ; tõlk. Triin Tael

    Index Scriptorium Estoniae

    Anderson, Gillian

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008. Intervjuu ilmus juulis portaalis USA Weekend

  8. Väliskülalistele jäi silma eestlaste suur armastus oma teatri vastu / Ivar Põllu

    Index Scriptorium Estoniae

    Põllu, Ivar, 1974-

    2005-01-01

    10. sept. lõppenud festivali "Draama 2005" laureaatidest. Parima lavastuse auhinna pälvis T. Ojasoo ja E.-L. Semperi "Julia" ja väliskriitikute eriauhinna E. Nüganeni "Tõde ja õigus. Teine osa". Lisatud laureaatide nimekiri. Vt. ka lk..2 "Draama läbi. Draamata"

  9. Noorteromaani konkursil jagati 60 000 krooni / Heili Sibrits

    Index Scriptorium Estoniae

    Sibrits, Heili, 1977-

    2010-01-01

    Eesti Lastekirjanduse Keskuse ja kirjastuse Tänapäev algupärase noorsooromaani võistluse peapreemia sai Reeli Reinausi romaan "Must vares", teine koht Kätlin Priilinn "Sefiirist loss", kolmas koht Marianne Harju "Talvehullus" ja Kristel Kriisa "Neetud"

  10. Reference: 304 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available genous carbon sources. Expression patterns of the plastid-encoded genes in the corr...teins (pTACs), have not yet been described. T-DNA insertions in three corresponding genes, ptac2, -6, and -12, are lethal without exo

  11. Piratskije istorii - eto nastojashtsheje zolotoje dno / Ilja Svensson

    Index Scriptorium Estoniae

    Svensson, Ilja

    2006-01-01

    Gore Verbinski lavastatud piraadi-triloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" ("Pirates of the Caribbean : Dead Man's Chest") : Ameerika Ühendriigid 2006. Ülevaatlikult ka esimese filmiga seonduvast. Augustis lõppevad kolmanda filmi võtted

  12. Proteins in similarity relationship with the cluster - Gclust Server | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Gclust Server Proteins in similarity relationship with the cluster Data detail Data name Pro...teins in similarity relationship with the cluster DOI 10.18908/lsdba.nbdc00464-003 Description of data conte...s Proteins in similarity relationship with the cluster - Gclust Server | LSDB Archive ...

  13. Kütioru Avatud Ateljee maailmas

    Index Scriptorium Estoniae

    1999-01-01

    Londoni ajakiri 'Casa Vogue' avaldab millenniuminumbri kaaneloona ülevaate Peeter ja Leelo Lauritsa juhitavast Kütioru Avatud Ateljeest. 4.-5. XII toimub Amsterdamis organisatsiooni Artists in Nature teine konverents, millel osaleb Kütioru Avatud Ateljee. Peeter Laurits ja Terje Ojaver on kutsutud osalema 5. XI avataval näitusel 'Diaspora' Oviedo linnas Hispaanias

  14. GenBank blastx search result: AK061773 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061773 001-039-D01 AF072475.1 Megasphaera elsdenii electron-transferring flavopro...tein b subunit (etfB) and electron-transferring flavoprotein a subunit (etfA) genes, complete cds.|BCT BCT 4e-27 +3 ...

  15. Saak on salves, teatriparemik teada / Agni Lass

    Index Scriptorium Estoniae

    Lass, Agni

    2005-01-01

    10. sept. lõppenud festivali "Draama 2005" laureaatidest. Parima lavastuse auhinna pälvis T. Ojasoo ja E.-L. Semperi "Julia" ja väliskriitikute eriauhinna E. Nüganeni "Tõde ja õigus. Teine osa". Lisatud laureaatide nimekiri, samuti teatriplakativõistluse laureaadid

  16. Raznõje litsa Indreka Paasa / Eteri Kekelidze

    Index Scriptorium Estoniae

    Kekelidze, Eteri, 1944-

    2008-01-01

    Vargamäel 22 tundi kestnud maratonetendusest Tammsaare "Tõe ja õiguse" põhjal - Elmo Nüganeni lavastused "Tõde ja õigus. Teine osa", "Karin. Indrek. Tõde ja õigus. 4" ja "Wargamäe Wabariik" (III osa) ning Jaanus Rohumaa lavastus "Vargamäe kuningriik" (I ja V osa). Pikemalt "Wargamäe Wabariigi" lavastusest

  17. Unusual structure and splicing pattern of the vertebrate ...

    Indian Academy of Sciences (India)

    ROSA CALVELLO

    2018-03-08

    Mar 8, 2018 ... teins (mitochondrial phosphate carriers) play an essential role in oxidative ... top most of the 22 mismatches occur at the third nucleotide of the coding ... cate sections, namely the upstream copy in the 'standard' transcript .... 2007; Shah and ..... logic groups: divergence times of eutherian mammals. Science.

  18. Vladimir Võssotski : planeta? ulitsa? tsvetok! / Deniss Ushakov

    Index Scriptorium Estoniae

    Ushakov, Deniss

    2008-01-01

    Vladimir Võssotski 70. sünniaastapäeva puhul meenutatakse viise, kuidas on püütud ja püütakse jäädvustada tema mälestust (raamatud, näitused, muuseumid, ausambad, tänavad jne). Sõna saavad ka poeg Nikita Võssotski ja teine abikaasa Ljudmila Abramova

  19. Ülihiidtäht Betelgeuse kaotab massi / Jüri Ivask

    Index Scriptorium Estoniae

    Ivask, Jüri, 1961-

    2009-01-01

    Betelgeuse, heleduselt teine täht Orioni tähtkujus, on punane ülihiid - üks suurimaid tähti, mida teatakse. Olles vaid mõne miljoni aasta vanune, läheneb Betelgeuse juba oma eluea lõpule ning peab peagi plahvatama supernoovana

  20. Pakistani juht sepitseb endale teist ametiaega / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2007-01-01

    Autori sõnul üritab Pakistani president Pervez Musharraf saavutada oma tagasivalimist lubadusega hakata tsiviilisikust presidendiks. Võimud on ähvardanud maapaost naasva endise Pakistani peaministri Benazir Bhutto vangi panna, juba on välja saadetud teine ekspeaminister Nawaz Sharif. Lisa: Kodusõja oht. Piirialad ähvardavad lahku lüüa

  1. Surnud mehe käsi / Veiko Belials

    Index Scriptorium Estoniae

    Belials, Veiko, 1966-

    1997-01-01

    Arvustus: Asimov, Isaac. Asum / inglise k. tlk. Linda Ariva. Tallinn : Eesti Raamat, 1985. (Mirabilia); Asimov, Isaac. Asum ja impeerium / inglise k. tlk. Linda Ariva. Tallinn : Eesti Raamat, 1989. (Mirabilia); Asimov, Isaac. Teine asum / inglise k. tlk. Indrek Elling. Tallinn : Eesti Raamat, 1996. (Mirabilia)

  2. The hepatitis C virus persistence: how to evade the immune system?

    Indian Academy of Sciences (India)

    Unknown

    1.1b HCV proteins and functions: The first structural protein, from the N-terminus of ... sera of the patients are associated with lipids and lipopro- teins, the nonspecific ...... Classification of hepatitis C virus into six major genotypes and a series of ...

  3. 500-tonniste muldseintega elamu / Ene Läkk, Helen Urbanik

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Arhitekt Rick Joy projekteeritud arst John Palmeri villa USAs Arizona osariigis, Santa Catalina mäestiku jalamil. Hoone ühes osas asuvad perekonna eluruumid, teine on avatud ruum. Pressitud muldseinad ja liblikatiiva kujulised terasest katusesiilud jäljendavad ümbritseva maastiku vorme. Suuri seina- ja aknapindu katab raamimata klaas.

  4. Kolm Eesti teost võistleb Prix Europa festivalil / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2004-01-01

    Tänavusel televisiooni ja raadiotööde festivalil kandideerivad 240 töö hulgas ka Liivo Niglase dokumentaalfilm "Juri Vella maailm" ja Tamur Tohveri lavastatud kaks raadiokuuldemängu "Boob teab" ja "Üks teine lugu"

  5. Noored eestlased Ladina-Ameerika narkokatlas / Krister Kivi

    Index Scriptorium Estoniae

    Kivi, Krister, 1975-

    2007-01-01

    Ladina-Ameerika vanglates kannab praegu karistust 23 Eesti päritolu narkokullerit. Autor külastas Eestist pärit kinnipeetavaid Quito vanglas ning vestles naisega, kelle poeg kannab karistust Venezuelas. Vt. samas: Janar Filippov. Kullerite värbajaid pole tabatud; "Narkomuulade" teine laine

  6. Mockus goes to run-off in Colombian presidential election / Rokas M. Tracevskis

    Index Scriptorium Estoniae

    Tracevskis, Rokas M.

    2010-01-01

    Kolumbias 30. mail toimunud presidendivalimistel sai Leedu juurtega Antanas Mockus 21% häältest, tema peamine vastane Juan Manuel Santos sai aga 47% häältest. Presidendivalimiste teine voor toimub 20. juunil. Poola presidendi Bronislav Komorowski ja Kreeka peaministri George Papandreu Leedu-juurtest

  7. Piratõ Karibskogo morja obideli tuzemtsev / Jekaterina Zhivova

    Index Scriptorium Estoniae

    Zhivova, Jekaterina

    2006-01-01

    Gore Verbinski lavastatud piraadi-triloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" ("Pirates of the Caribbean : Dead Man's Chest") : Ameerika Ühendriigid 2006. Ülevaatlikult ka esimese filmiga seonduvast. Augustis lõppevad kolmanda filmi võtted

  8. Selles töös oli kaks õudust : vangla ja tants / Mait Malmsten ; interv. Tiina Ansip

    Index Scriptorium Estoniae

    Malmsten, Mait

    2005-01-01

    Näitleja räägib oma tööst ja rollist rahvusvahelises ühistöös valminud tantsufilmis "Teine" ("Another") : režissöör Rene Vilbre : koreograaf ja peaoaline Helena Jonsdottir. Filmi võtted toimusid Tartu vanglas

  9. Võtme ja riiviga tantsufilm / Kristiina Garancis

    Index Scriptorium Estoniae

    Garancis, Kristiina, 1969-

    2005-01-01

    Rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre : stsenaristid Rene Vilbre, Teet Kask, Helena Jonsdottir : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir : osatäitjad Mait Malmsten, Ruslan Stepanov : Eesti - Island 2005

  10. Sõpruses esilinastub Eesti-Islandi tantsufilm

    Index Scriptorium Estoniae

    2005-01-01

    10. novembril esilinastub rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre : stsenaristid Rene Vilbre, Teet Kask, Helena Jonsdottir : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir : osatäitjad Mait Malmsten, Ruslan Stepanov. Filmi võtted toimusid Tartu vanglas

  11. Vanglarahvas tantsib Euroopasse / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2005-01-01

    Rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre : stsenaristid Rene Vilbre, Teet Kask, Helena Jonsdottir : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir : osatäitjad Mait Malmsten, Ruslan Stepanov. Filmi võtted toimusid Tartu vanglas

  12. Rohkem kui lihtsalt tantsufilm / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2005-01-01

    Rahvusvahelises ühistöös valminud tantsufilm "Teine" ("Another") : režissöör Rene Vilbre : stsenaristid Rene Vilbre, Teet Kask, Helena Jonsdottir : produtsent Heidi Pruuli : koreograaf ja peaoaline Helena Jonsdottir : osatäitjad Mait Malmsten, Ruslan Stepanov : Eesti - Island 2005

  13. EMT võrgurikke tõttu jäi 750 000 mobiiltelefoni tummaks / Raigo Neudorf

    Index Scriptorium Estoniae

    Neudorf, Raigo

    2010-01-01

    Eesti suurima mobiilside operaatori EMT tuumikvõrku tabas rike, mille tõttu oli umbes pooleteise tunni jooksul häiritud ligi kolmveerand miljoni kliendi sideühendus. EMT on Elioni kõrval juba teine suurfirma, keda viimasel ajal on tabanud ulatuslik katkestus

  14. Неполадки в EMT оставили без связи 750 000 человек / Райго Неудорф

    Index Scriptorium Estoniae

    Неудорф, Райго

    2010-01-01

    Eesti suurima mobiilside operaatori EMT tuumikvõrku tabas rike, mille tõttu oli umbes pooleteise tunni jooksul häiritud ligi kolmveerand miljoni kliendi sideühendus. EMT on Elioni kõrval juba teine suurfirma, keda viimasel ajal on tabanud ulatuslik katkestus

  15. Minimalistlikud muinasjutud / Veiko Märka

    Index Scriptorium Estoniae

    Märka, Veiko, 1964-

    2007-01-01

    Sisu: Feodaalkord Lapimaal ; Taimetoitlase õudusunenägu ; Savisaare pilt ; Mis mulle meenub, nähes lepatriinut? ; Viagra võluvägi ; Uskumatu loomaporno ; Tunne oma kodumaad ; Tunne oma kodumaad 2. ; Üleskutse Polüneesia puuviljakasvatajatele ; EELK peapiiskopi maja ; Stalingradi lahing ; Tark mees ; Teine Arnold ; Kolmas Arnold

  16. "Head käed" pääses Berliini filmifestivalile / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2002-01-01

    Peeter Simmi mängufilmi näidatakse järgmisel Berliini filmifestivalil Panorama programmis. Teine eesti mängufilm - Arvo Iho "Karu süda" linastub vaid festivali filmiturul. Ühelgi rahvusvahelisel festivalil film siiani pole osalenud

  17. Gedichte aus den Jahren 1968 bis 1975 / Viivi Luik ; tõlk. Gisbert Jänicke

    Index Scriptorium Estoniae

    Luik, Viivi, 1946-

    1999-01-01

    Tekst saksa ja eesti keeles. Sisu: Weite = Avarus ; "Wie der Krieg..." = "Otsekui sõda..." ; "es vergehn..." = "hävivad..." ; Das Geheimnis = Saladus ; "Ob auch ich weiss..." = "Kas minagi tean..." ; "Ich redete einmal von Feldern..." = "Rääkisin väljadest ükskord..." ; Andere = Teine ; Der Tag = Päev

  18. "Russkii bunt" smetajut s polok / Konstantin Morenko

    Index Scriptorium Estoniae

    Morenko, Konstantin

    2007-01-01

    Urmas E. Liivi dokumentaalfilm "Pronksöö : Vene mäss Tallinnas" DVD tuli müügile 9. juulil. Esimene tiraazh (üle 4000) müüdi läbi kuu ajaga, tellitud on teine (3000), millest osa loodetakse müüa väljaspoole Eestit

  19. Mereröövlid saabusid suvist letargiat maha raputama / Mari Rebane

    Index Scriptorium Estoniae

    Rebane, Mari

    2006-01-01

    Esilinastus Gore Verbinski piraaditriloogia teine film "Kariibi mere piraadid : Surnud mehe aardekirst" : Ameerika Ühendriigid 2006. Lisaks lühendatud artikkel Los Angeles Times 10. juuli Josh Friedman "Piraadid püstitasid kassarekordi" ja katke ajalehele Guardian antud intervjuust Johnny Deppiga "Kapten Jack Sparrow laseb Deppil pingeid maandada"

  20. Kunstnike aastanäitus kui kobarkino

    Index Scriptorium Estoniae

    2003-01-01

    19. detsembrist Tallinnas Eesti Kunstnike Liidu IV aastanäitus: Hobusepea ja Draakoni galeriis Andrus Joonase kuraatoriprojekt "Rehabilitatsioon" (avamisel esineb rühmitus Fashist Lendas Üle), Tallinna Kunstihoones ja Kunstihoone galeriis Ants Juske kureeritud "Mina ja Teine" (kujundaja Jaan Elken, avamisel esitles Eesti Litograafiakeskus oma uut litomappi)

  1. Noorte lühinäidendid jahmatasid publikut / Maria Uuetoa

    Index Scriptorium Estoniae

    Uuetoa, Maria

    2007-01-01

    Eesti Nuku- ja Noorsooteater tõi lavale kolmest lühinäidendist koosneva etenduse "Põlev pööning". Näidendivõistluselt "Pööning" valitud kolm näidendit on Kristiina Jalasto "Kui mind ei oleks, oleks maailm teine" (lav. Andres Dvinjaninov), Priit Põldma "Koerad" (lav. Vahur Keller) ja Maili Lehtpuu "Koristajad" (lav. Reeda Toots)

  2. Genetic variability and relationship between MT-1 elephant grass ...

    Indian Academy of Sciences (India)

    tein, 28 to 40 per cent in crude fiber, 10 to 16 per cent in ash, 0.9 ... and forage production in the alley cropping system of agro- ... Keywords. genetic diversity; cluster analysis; DNA markers; Shannon information index; Pennisetum purpureum.

  3. Savisaare jõuluime taustaks on valimiseelne leping Kruudaga / Hannes Krause

    Index Scriptorium Estoniae

    Krause, Hannes

    2005-01-01

    Keskerakonna esimehele Edgar Savisaarele Keila-Joal asuva suvila müümisest alla turuhinna. Kommenteerivad Ivar Tallo ja Margus Leivo. Lisad: Savisaare majatehing; Tehing; Savisaarel teine majaskandaal. Vt. samas: Hannes Krause, Jaanus Piirsalu. Aruanne võib näidata maja õiget hinda; Hannes Krause, Kristjan Jõevere. Savisaare omaga sarnane maja müügis 3 miljoniga

  4. Tougher times for the ruling center-right / Rokas M. Tracevskis

    Index Scriptorium Estoniae

    Tracevskis, Rokas M.

    2010-01-01

    Viimaste sisepoliitiliste muutuste tagajärjel on Leedus praegu võimul vähemusvalitsus. Kui parlament avaldab umbusaldust Linas Karaliusele ja Aleksandr Sacharukile, kellest esimene puudus istungitelt ja teine hääletas parlamendis tema eest, võib koalitsioon saada parlamendis kaks lisakohta. Endise Leedu suursaadiku Gruusias arvamus

  5. Dan Põldroos mängib Eesti superkangelast / Eve Heinla

    Index Scriptorium Estoniae

    Heinla, Eve, 1966-

    2001-01-01

    Eestlaste filmigrupp filmis Tuneesias esimest osa kodumaisest koomilisest märuliseriaalist "Kodumaa parim poeg ehk eriagent 1188" : stsenarist Toomas Kall : režissöör Peeter Simm : peaosades näitleja ja sarja produtsent Dan Põldroos ning teine režissöör Ervin Õunapuu

  6. Understanding protein–protein interactions by genetic suppression

    Indian Academy of Sciences (India)

    Unknown

    Protein–protein interactions influence many cellular processes and it is increasingly being felt that even a weak and ... In a bacterial system where the complete genome sequence is available, it is an arduous ... teins (primary mutations) are useful in these studies. ... of interaction of this antibiotic with the central enzyme.

  7. "Situated Self" käsitleb poliitilise agoraa tagamaid / Branislav Dimitrijevich ; interv. Rael Artel

    Index Scriptorium Estoniae

    Dimitrijević, Branislav, 1967-

    2005-01-01

    Üks kuraatoritest näitusest "Situated Self : Confused, Compassionate and Conflictful", mis toimus veebruaris märtsis Belgradi kaasaegse kunsti muuseumis ja mais-juunis Helsingi Tennispalatsis. Projekti teine kuraator Mika Hannula. Eksponeeriti 20 Euroopa perifeeria kunstniku loomingut

  8. Euroopa tellisepreemia 2006 / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2006-01-01

    Viinis toimunud Euroopa tellisarhitektuuri auhindade Brick'06 kätteandmisel märgiti ära Tartu Jaani kiriku taastamine (arhitekt Udo Tiirmaa, kunstiajaloolane Kaur Alttoa). Esimene koht: Ungari Pecsi linna Barbakani väljaku elu- ja ärikompleks (arhitektid Ferenc Csagoly, Ferenc Keller). Teine koht: kiriku rekonstruktsioon raamatukoguks Madridis (arhitekt Jose Ignacio Rodriguez)

  9. Semiootilisest ekspertiisist / Anti Randviir

    Index Scriptorium Estoniae

    Randviir, Anti, 1975-

    2006-01-01

    Tartu Ülikooli semiootikaosakonna vanemteadur käsitleb seoses K-kohukese ja "Kommarid ahju!" ekspertiisiga kahte aspekti: esimene puudutab semiootiku kui ametimehe ülesandeid, teine moment on semiootiku ja tavakodaniku suhted. "Kommarid ahju!" teksti analüüsist

  10. Lorena reeglid / Lorena Rojas ; interv. Kaisa Karu

    Index Scriptorium Estoniae

    Rojas, Lorena

    2007-01-01

    Mehhiko telenovelade staar, kes mängib ka sel hooajal TV3s jooksvas seriaalis "Teine võimalus" ("El cuerpo del deseo"), valiti ajak. People poolt 2006.a. maailma 100 ilusama naise hulka. Sama : Teleleht nr. 3, lk. 38 : ill

  11. Keegi ei ela eksiilis, kõik elavad kodus / Sirje Helme

    Index Scriptorium Estoniae

    Helme, Sirje, 1949-

    2004-01-01

    Tallinna XIII graafikatriennaalist. Põhinäitused: Rotermanni soolalaos "Maapagu", mis hõlmab rahvusvahelise žürii valitud näitust ja Peeter Alliku, Redas Dirzhyse, Guntars Sietinshi ning Juris Petraskevici kureeritud balti graafika näitust, Tallinna Kunstihoones ning Ku galeriis Sirje Helme kureeritud "Teine naaber"

  12. ANCEI

    Indian Academy of Sciences (India)

    new interpretations." Indeed, when Beadle left ... of being able to combine genetics and develop- opmental ... Yet, the latter field came of own right, in addition to being of practical value its own much ... are related to the physiology of the brain. We knoweven ... genes encode proteins, and .. the function of pro- teins affects ...

  13. Berlinalel hõrku kinokunsti maitsmas / Annika Koppel

    Index Scriptorium Estoniae

    Koppel, Annika

    2007-01-01

    57. Berliini filmifestivali auhinnatud filmidest. Peaauhind Kuldkaru - hiina režissööri Wang Quanani film "Tuya abielu" ("Tuya de hun shi"), parima režissööri Hõbekaru - iisraellane Joseph Cedar ("Beafort"), parim meesnäitleja - Julio Chavez (Ariel Rotteri "Teine"), parim naisnäitleja - Nina Hossa (Christian Petzoldi "Yella")

  14. Näitus Mahtra talurahvamuuseumi arhitektuurikonkursile laekunud töödest

    Index Scriptorium Estoniae

    2005-01-01

    Võistlustööde hindamiskomisjoni liikmed. Parim töö "Heinamaa", arhitektid Tomomi Hayashi ja Hanno Grossschmidt arhitektuuribüroost HG Arhitektuur. Teine koht Andres Lemberi ja Tarmo Maiste tööle "Talgud", kolmas koht Liina Jaanuse tööle "Pärand", neljas koht Indrek Järve tööle "Part"

  15. Lionsi asepresident kiitis Eesti "lõvisid" / Marika Milve

    Index Scriptorium Estoniae

    Milve, Marika, 1968-

    1995-01-01

    Lions klubi Eestis. Vt. ka Meie Maa "Lionsite maailma-assotsiatsiooni teine asepresident Augustino Solivo külastas Saaremaad" 8- juuni, lk. 1, Oma Saar "Augustino Soliva: tavaliselt oskavad inimesed võtta, kuid ei oska anda" 7. juuni, lk. 3

  16. 2x väikesest saab suur / Varje Talivee

    Index Scriptorium Estoniae

    Talivee, Varje

    2011-01-01

    Sisearhitekt Aet Piel kujundas osavate kujundusvõtetega väikesest 43 m² kahetoalisest tüüpkorterist kaks ruumikalt mõjuvat tervikut, millest üks on elegantselt hall ning teine valge ja helge. Mõlemaid näidislahendusi esitleti sisustusmessil Interjöör 2011

  17. Krugovrashtshenije pobegov v prirode / Nikolai Hrustaljov

    Index Scriptorium Estoniae

    Hrustaljov, Nikolai

    2008-01-01

    Venemaal, Ivanovski oblastis Jurjevetsi toimus teine Andrei Tarkovskile pühendatud filmifestival "Peegel" ("Zerkalo"). Pikemalt Hiner Saleemi filmist "Pariisi katuste all" ("Sous les toits de Paris") : Prantsusmaa 2007 ja Csaba Bolloki filmist "Iska teekond" ("Iszka utazasa") : Ungari 2007. Ka teistest auhinnavõitjatest

  18. Vargamäelt leigust ei leitud / Jan Kaus

    Index Scriptorium Estoniae

    Kaus, Jan, 1971-

    2008-01-01

    Vargamäel 21. juunil 22 tundi kestnud maratonetendusest Tammsaare "Tõe ja õiguse" põhjal - Elmo Nüganeni lavastused "Tõde ja õigus. Teine osa", "Karin. Indrek. Tõde ja õigus. 4" ja "Wargamäe Wabariik" (III osa) ning Jaanus Rohumaa lavastus "Vargamäe kuningriik" (I ja V osa)

  19. IKEA teeb panuse Briti ja Venemaa turule / Tõnis Arnover

    Index Scriptorium Estoniae

    Arnover, Tõnis, 1952-

    2006-01-01

    Rootsi mööblikontsern IKEA laiendab müügipindu Suurbritannias, Venemaal ja Jaapanis. Diagramm: Suurbritannia turg IKEA-le ka praegu tähtsuselt teine. Vt. samas: IKEA firmakultuur ei näe ette juhtivtöötajate poputamist

  20. Tartu rahvusvaheliseks kunstilinnaks! / Kaisa Eiche ; intervjueerinud Reet Varblane

    Index Scriptorium Estoniae

    Eiche, Kaisa, 1982-

    2010-01-01

    Tartu kunstikuu organisaator Kaisa Eiche 8. septembrist 2. oktoobrini 2010 toimuvast kunstikuust-festivalist "ART IST KUKU NU UT", mille teine korraldaja on Rael Artel. Pikemalt trükisest "ART IST KUKU NU UT" ja 8. septembril Y-galeriis avatavast Rael Arteli kureeritud rahvusvahelisest näitusest "Lisa 6. Nähtamatu käe poliitika" (osalejad loetletud)