WorldWideScience

Sample records for technology macro-scale dm

  1. Electromagnetic DM technology meets future AO demands

    Science.gov (United States)

    Hamelinck, Roger; Rosielle, Nick; Steinbuch, Maarten; Doelman, Niek

    New deformable mirror technology is developed by the Technische Universiteit Eindhoven, Delft University of Technology and TNO Science and Industry. Several prototype adaptive deformable mirrors are realized mirrors, up to 427 actuators and ∅150mm diameter, with characteristics suitable for future AO systems. The prototypes consist of a 100µm thick, continuous facesheet on which low voltage, electromagnetic, push-pull actuators impose out-of-plane displacements. The variable reluctance actuators with ±10µm stroke and nanometer resolution are located in a standard actuator module. Each module with 61 actuators connects to a single PCB with dedicated, 16 bit, PWM based, drivers. A LVDS multi-drop cable connects up to 32 actuator modules. With the actuator module, accompanying PCB and multi-drop system the deformable mirror technology is made modular in its mechanics and electronics. An Ethernet-LVDS bridge enables any commercial PC to control the mirror using the UDP standard. Latest results of the deformable mirror technology development are presented.

  2. Diffusiophoresis at the macro-scale

    CERN Document Server

    Mauger, C; Machicoane, N; Bourgoin, M; Cottin-Bizonne, C; Ybert, C; Raynal, F

    2015-01-01

    Diffusiophoresis, a ubiquitous phenomenon which induces particle transport whenever solute gradients are present, was recently put forward in the context of microsystems and shown to strongly impact colloidal transport (from patterning to mixing) at such scales. In the present work, we show experimentally that this nanoscale-rooted mechanism can actually induce changes in the \\textit{macro-scale mixing} of colloids by chaotic advection. Rather than the usual decay of standard deviation of concentration, which is a global parameter, we use different multi-scale tools available for chaotic flows or intermittent turbulent mixing, like concentration spectra, or second and fourth moments of probability density functions of scalar gradients. Not only those tools can be used in open flows (when the mean concentration is not constant), but also they allow for a scale by scale analysis. Strikingly, diffusiophoresis is shown to affect all scales, although more particularly the smallest one, resulting in a change of sca...

  3. Characteristics of soil water retention curve at macro-scale

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Scale adaptable hydrological models have attracted more and more attentions in the hydrological modeling research community, and the constitutive relationship at the macro-scale is one of the most important issues, upon which there are not enough research activities yet. Taking the constitutive relationships of soil water movement--soil water retention curve (SWRC) as an example, this study extends the definition of SWRC at the micro-scale to that at the macro-scale, and aided by Monte Carlo method we demonstrate that soil property and the spatial distribution of soil moisture will affect the features of SWRC greatly. Furthermore, we assume that the spatial distribution of soil moisture is the result of self-organization of climate, soil, ground water and soil water movement under the specific boundary conditions, and we also carry out numerical experiments of soil water movement at the vertical direction in order to explore the relationship between SWRC at the macro-scale and the combinations of climate, soil, and groundwater. The results show that SWRCs at the macro-scale and micro-scale presents totally different features, e.g., the essential hysteresis phenomenon which is exaggerated with increasing aridity index and rising groundwater table. Soil property plays an important role in the shape of SWRC which will even lead to a rectangular shape under drier conditions, and power function form of SWRC widely adopted in hydrological model might be revised for most situations at the macro-scale.

  4. Discrete-element method simulations: from micro to macro scales.

    Science.gov (United States)

    Heyes, D M; Baxter, J; Tüzün, U; Qin, R S

    2004-09-15

    Many liquid systems encountered in environmental science are often complex mixtures of many components which place severe demands on traditional computational modelling techniques. A meso scale description is required to account adequately for their flow behaviour on the meso and macro scales. Traditional techniques of computational fluid dynamics and molecular simulation are not well suited to tackling these systems, and researchers are increasingly turning to a range of relatively new computational techniques that offer the prospect of addressing the factors relevant to multicomponent multiphase liquids on length- and time-scales between the molecular level and the macro scale. In this category, we discuss the off-lattice techniques of 'smooth particle hydrodynamics' (SPH) and 'dissipative particle dynamics' (DPD), and the grid-based techniques of 'lattice gas' and 'lattice Boltzmann' (LB). We highlight the main conceptual and technical features underpinning these methods, their strengths and weaknesses, and provide a few examples of the applications of these techniques that illustrate their utility.

  5. Investigation of Micro- and Macro-Scale Transport Processes for Improved Fuel Cell Performance

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Wenbin

    2015-02-05

    This report documents the work performed by General Motors (GM) under the Cooperative agreement No. DE-EE0000470, “Investigation of Micro- and Macro-Scale Transport Processes for Improved Fuel Cell Performance,” in collaboration with the Penn State University (PSU), University of Tennessee Knoxville (UTK), Rochester Institute of Technology (RIT), and University of Rochester (UR) via subcontracts. The overall objectives of the project are to investigate and synthesize fundamental understanding of transport phenomena at both the macro- and micro-scales for the development of a down-the-channel model that accounts for all transport domains in a broad operating space. GM as a prime contractor focused on cell level experiments and modeling, and the Universities as subcontractors worked toward fundamental understanding of each component and associated interface.

  6. Investigation of Micro- and Macro-Scale Transport Processes for Improved Fuel Cell Performance

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Wenbin [General Motors LLC, Pontiac, MI (United States)

    2014-08-29

    This report documents the work performed by General Motors (GM) under the Cooperative agreement No. DE-EE0000470, “Investigation of Micro- and Macro-Scale Transport Processes for Improved Fuel Cell Performance,” in collaboration with the Penn State University (PSU), University of Tennessee Knoxville (UTK), Rochester Institute of Technology (RIT), and University of Rochester (UR) via subcontracts. The overall objectives of the project are to investigate and synthesize fundamental understanding of transport phenomena at both the macro- and micro-scales for the development of a down-the-channel model that accounts for all transport domains in a broad operating space. GM as a prime contractor focused on cell level experiments and modeling, and the Universities as subcontractors worked toward fundamental understanding of each component and associated interface.

  7. Toward Zero Micro/Macro-Scale Wear Using Periodic Nano-Layered Coatings.

    Science.gov (United States)

    Penkov, Oleksiy V; Devizenko, Alexander Yu; Khadem, Mahdi; Zubarev, Evgeniy N; Kondratenko, Valeriy V; Kim, Dae-Eun

    2015-08-19

    Wear is an important phenomenon that affects the efficiency and life of all moving machines. In this regard, extensive efforts have been devoted to achieve the lowest possible wear in sliding systems. With the advent of novel materials in recent years, technology is moving toward realization of zero wear. Here, we report on the development of new functional coatings comprising periodically stacked nanolayers of amorphous carbon and cobalt that are extremely wear resistant at the micro and macro scale. Because of their unique structure, these coatings simultaneously provide high elasticity and ultrahigh shear strength. As a result, almost zero wear was observed even after one million sliding cycles without any lubrication. The wear rate was reduced by 8-10-fold compared with the best previously reported data on extremely low wear materials.

  8. Macro Scale Independently Homogenized Subcells for Modeling Braided Composites

    Science.gov (United States)

    Blinzler, Brina J.; Goldberg, Robert K.; Binienda, Wieslaw K.

    2012-01-01

    An analytical method has been developed to analyze the impact response of triaxially braided carbon fiber composites, including the penetration velocity and impact damage patterns. In the analytical model, the triaxial braid architecture is simulated by using four parallel shell elements, each of which is modeled as a laminated composite. Currently, each shell element is considered to be a smeared homogeneous material. The commercial transient dynamic finite element code LS-DYNA is used to conduct the simulations, and a continuum damage mechanics model internal to LS-DYNA is used as the material constitutive model. To determine the stiffness and strength properties required for the constitutive model, a top-down approach for determining the strength properties is merged with a bottom-up approach for determining the stiffness properties. The top-down portion uses global strengths obtained from macro-scale coupon level testing to characterize the material strengths for each subcell. The bottom-up portion uses micro-scale fiber and matrix stiffness properties to characterize the material stiffness for each subcell. Simulations of quasi-static coupon level tests for several representative composites are conducted along with impact simulations.

  9. Dermatomyositis (DM)

    Science.gov (United States)

    ... Favorite Name: Category: Share: Yes No, Keep Private Dermatomyositis Share | Dermatomyositis (DM) is a rare inflammatory muscle disease that ... drugs, and infectious agents) in genetically predisposed individuals. Dermatomyositis can occur with other connective tissue disorders such ...

  10. From micro-scale 3D simulations to macro-scale model of periodic porous media

    Science.gov (United States)

    Crevacore, Eleonora; Tosco, Tiziana; Marchisio, Daniele; Sethi, Rajandrea; Messina, Francesca

    2015-04-01

    In environmental engineering, the transport of colloidal suspensions in porous media is studied to understand the fate of potentially harmful nano-particles and to design new remediation technologies. In this perspective, averaging techniques applied to micro-scale numerical simulations are a powerful tool to extrapolate accurate macro-scale models. Choosing two simplified packing configurations of soil grains and starting from a single elementary cell (module), it is possible to take advantage of the periodicity of the structures to reduce the computation costs of full 3D simulations. Steady-state flow simulations for incompressible fluid in laminar regime are implemented. Transport simulations are based on the pore-scale advection-diffusion equation, that can be enriched introducing also the Stokes velocity (to consider the gravity effect) and the interception mechanism. Simulations are carried on a domain composed of several elementary modules, that serve as control volumes in a finite volume method for the macro-scale method. The periodicity of the medium involves the periodicity of the flow field and this will be of great importance during the up-scaling procedure, allowing relevant simplifications. Micro-scale numerical data are treated in order to compute the mean concentration (volume and area averages) and fluxes on each module. The simulation results are used to compare the micro-scale averaged equation to the integral form of the macroscopic one, making a distinction between those terms that could be computed exactly and those for which a closure in needed. Of particular interest it is the investigation of the origin of macro-scale terms such as the dispersion and tortuosity, trying to describe them with micro-scale known quantities. Traditionally, to study the colloidal transport many simplifications are introduced, such those concerning ultra-simplified geometry that usually account for a single collector. Gradual removal of such hypothesis leads to a

  11. Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture.

    Science.gov (United States)

    Son, Jaejung; Bae, Chae Yun; Park, Je-Kyun

    2016-01-11

    Hydrogels can be patterned at the micro-scale using microfluidic or micropatterning technologies to provide an in vivo-like three-dimensional (3D) tissue geometry. The resulting 3D hydrogel-based cellular constructs have been introduced as an alternative to animal experiments for advanced biological studies, pharmacological assays and organ transplant applications. Although hydrogel-based particles and fibers can be easily fabricated, it is difficult to manipulate them for tissue reconstruction. In this video, we describe a fabrication method for micropatterned alginate hydrogel sheets, together with their assembly to form a macro-scale 3D cell culture system with a controlled cellular microenvironment. Using a mist form of the calcium gelling agent, thin hydrogel sheets are easily generated with a thickness in the range of 100 - 200 µm, and with precise micropatterns. Cells can then be cultured with the geometric guidance of the hydrogel sheets in freestanding conditions. Furthermore, the hydrogel sheets can be readily manipulated using a micropipette with an end-cut tip, and can be assembled into multi-layered structures by stacking them using a patterned polydimethylsiloxane (PDMS) frame. These modular hydrogel sheets, which can be fabricated using a facile process, have potential applications of in vitro drug assays and biological studies, including functional studies of micro- and macrostructure and tissue reconstruction.

  12. Monitoring of frozen soil hydrology in macro-scale in the Qinghai-Xizang Plateau

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Monitoring of frozen soil hydrology in macro-scale was performed by Chinese and Japanese scientists from 1997 to 1998. Quality measured data were obtained. Measured data on soil moisture and temperature are preliminarily analyzed. Based on profiles of soil temperature and moisture in individual measured sites, intra-annual freezing and melting process of soil is discussed. Maximum frozen and thawed depths and frozen days in various depths are estimated. The work emphasized the spatial distribution on soil temperature and moisture in macro-scale and the effect of topography on conditions of soil water and heat.

  13. Implementation of Microstructural Material Phenomena in Macro Scale Simulations of Forming Processes

    NARCIS (Netherlands)

    Huetink, J.

    2004-01-01

    The paper deals with problems related to full/macro scale simulations of industrial forming processes. Large-scale numerical simulations and virtual modeling are replacing prototypes in order to reduce costs and time. This requires accurate and reliable predictions. To satisfy these requirements, so

  14. Micro and macro scale electrohydrodynamic enhancement of thin-film evaporation

    Science.gov (United States)

    Darabi, Jafar

    2000-11-01

    Evaporation of thin liquid films has long been recognized as one of the most effective methods of heat removal. As a result, techniques that employ this mechanism have potential for use in many practical applications such as electronic cooling, heat pipes, and process heat exchangers. Demand for high-power density electronics, along with the associated requirements including temperature uniformity and the limitation on maximum temperature, will require the development of new methods of heat removal for these devices. The electrohydrodynamic (EHD) technique offers a promising alternative for the uniform distribution of temperature and the removal of heat at high power levels. These factors directly affect the performance, cost, and reliability of such devices. An experimental investigation was undertaken to study the feasibility of applying the EHD technique for heat transfer enhancement of thin-film evaporation. Macro-scale experiments were conducted on several heat transfer surfaces in both horizontal and vertical orientations and the mechanisms involved in heat transfer enhancement were clarified. For the various heat transfer surface/electrode geometries tested, enhancement factors ranging from 25% to 390% were obtained. The novel concept of EHD-enhanced source level cooling utilizing MEMS and thin-film evaporation was then introduced. The device was designed and fabricated using VLSI fabrication technology. This technology allowed the integration of an active cooling device, a micropump, and temperature sensors into a single chip, greatly facilitating the manufacturing process, increasing the cooling capacity, and improving the thermal management of future high-power density electronics. The results indicate a maximum cooling capacity of 65 W/cm2 and a corresponding pumping head of 250 Pa. This unique microcooling device has high commercialization potential and can pave the way for practical utilization of thin-film evaporation in microelectronics cooling and

  15. Handbook of damage mechanics nano to macro scale for materials and structures

    CERN Document Server

    2015-01-01

    This authoritative reference provides comprehensive coverage of the topics of damage and healing mechanics. Computational modeling of constitutive equations is provided as well as solved examples in engineering applications. A wide range of materials that engineers may encounter are covered, including metals, composites, ceramics, polymers, biomaterials, and nanomaterials. The internationally recognized team of contributors employ a consistent and systematic approach, offering readers a user-friendly reference that is ideal for frequent consultation. Handbook of Damage Mechanics: Nano to Macro Scale for Materials and Structures is ideal for graduate students and faculty, researchers, and professionals in the fields of Mechanical Engineering, Civil Engineering, Aerospace Engineering, Materials Science, and Engineering Mechanics.

  16. Effects of macro-scale uncertainties on the imaging and automatic manipulation of nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Korayem, M. H., E-mail: hkorayem@iust.ac.ir; Sadeghzadeh, S.; Homayooni, A. [Iran University of Science and Technology, Robotic Research Laboratory, School of Mechanical Engineering (Iran, Islamic Republic of)

    2013-01-15

    The steering, positioning, and fabrication operations in nano scale have been hampered by the uncertainties which come from the macro parts of nano-positioners. Among those uncertainties, the nonlinearities of piezo scanners have the highest contribution, which should be identified and compensated. On the other hand, the recognition of the effects of macro-scale nonlinearities on small-scale dynamics requires the simultaneous consideration of both the macro- and small-scale dynamics. This necessitates the implementation of multi-scale methods. In this article, a fixed interfacial multi-scale method (FIMM) that includes the effects of hysteresis has been used for the computationally and mathematically efficient modeling of nano-positioners. This method presents an improved coupling approach that can be used to study the imaging and manipulation of nanoparticles (from one to several hundred nanometers in diameter) subjected to nonlinear as well as linear positioning schemes. After comparing the applied hysteresis model with some previous experimental works, the dynamics of imaging and automatic manipulation of nanoparticles have been studied and some useful results have been presented. This paper opens a new window to the recognition and compensation of the errors of macro-scale nonlinearities imposed on small-scale dynamics.

  17. Meshing complex macro-scale objects into self-assembling bricks

    Science.gov (United States)

    Hacohen, Adar; Hanniel, Iddo; Nikulshin, Yasha; Wolfus, Shuki; Abu-Horowitz, Almogit; Bachelet, Ido

    2015-07-01

    Self-assembly provides an information-economical route to the fabrication of objects at virtually all scales. However, there is no known algorithm to program self-assembly in macro-scale, solid, complex 3D objects. Here such an algorithm is described, which is inspired by the molecular assembly of DNA, and based on bricks designed by tetrahedral meshing of arbitrary objects. Assembly rules are encoded by topographic cues imprinted on brick faces while attraction between bricks is provided by embedded magnets. The bricks can then be mixed in a container and agitated, leading to properly assembled objects at high yields and zero errors. The system and its assembly dynamics were characterized by video and audio analysis, enabling the precise time- and space-resolved characterization of its performance and accuracy. Improved designs inspired by our system could lead to successful implementation of self-assembly at the macro-scale, allowing rapid, on-demand fabrication of objects without the need for assembly lines.

  18. A macro-scale perspective on within-farm management: how climate and topography alter the effect of farming practices.

    Science.gov (United States)

    Amano, Tatsuya; Kusumoto, Yoshinobu; Okamura, Hiroshi; Baba, Yuki G; Hamasaki, Kenji; Tanaka, Koichi; Yamamoto, Shori

    2011-12-01

    Organic farming has the potential to reverse biodiversity loss in farmland and benefit agriculture by enhancing ecosystem services. Although the mixed success of organic farming in enhancing biodiversity has been attributed to differences in taxa and landscape context, no studies have focused on the effect of macro-scale factors such as climate and topography. This study provides the first assessment of the impact of macro-scale factors on the effectiveness of within-farm management on biodiversity, using spiders in Japan as an example. A multilevel modelling approach revealed that reducing pesticide applications increases spider abundance, particularly in areas with high precipitation, which were also associated with high potential spider abundance. Using the model we identified areas throughout Japan that can potentially benefit from organic farming. The alteration of local habitat-abundance relations by macro-scale factors could explain the reported low spatial generality in the effects of organic farming and patterns of habitat association.

  19. Digital holographic setups for phase object measurements in micro and macro scale

    Directory of Open Access Journals (Sweden)

    Lédl Vít

    2015-01-01

    Full Text Available The measurement of properties of so called phase objects is being solved for more than one Century starting probably with schlieren technique 1. Classical interferometry served as a great measurement tool for several decades and was replaced by holographic interferometry, which disposes with many benefits when compared to classical interferometry. Holographic interferometry undergone an enormous development in last decade when digital holography has been established as a standard technique and most of the drawbacks were solved. The paper deals with scope of the huge applicability of digital holographic interferometry in heat and mass transfer measurement from micro to macro scale and from simple 2D measurement up to complex tomographic techniques. Recently the very complex experimental setups are under development in our labs combining many techniques leading to digital holographic micro tomography methods.

  20. Predator-prey interactions as macro-scale drivers of species diversity in mammals

    DEFF Research Database (Denmark)

    Sandom, Christopher James; Sandel, Brody Steven; Dalby, Lars

    mechanistic drivers of mammal species richness at macro-scales for two trophic levels: predators and prey. To disentangle biotic (i.e. functional predator-prey interactions) from abiotic (i.e. environmental) and bottom-up from top-down determinants we considered three hypotheses: 1) environmental factors......-down). We gathered distributional range, mass and diet data for 4,091 terrestrial mammal species, excluding bats. Species richness maps were created for predators and prey and structural equation modelling was used to test the three hypotheses at continental and global scales. We also explored...... the importance of functional trait composition by analyzing richness of large and small mass categories for prey (division at 10 kg) and predators (division at 21.5 kg). Results/Conclusions Mammal species richness increased from the poles to the equator, supporting the classic latitudinal richness gradient...

  1. Effectiveness of technology-assisted case management in low income adults with type 2 diabetes (TACM-DM: study protocol for a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Strom Joni L

    2011-10-01

    Full Text Available Abstract Background An estimated 1 in 3 American adults will have diabetes by the year 2050. Nationally, South Carolina ranks 10th in cases of diagnosed diabetes compared to other states. In adults, type 2 diabetes (T2DM accounts for approximately 90-95% of all diagnosed cases of diabetes. Clinically, provider and health system factors account for Methods We describe a four-year prospective, randomized clinical trial, which will test the effectiveness of technology-assisted case management in low income rural adults with T2DM. Two-hundred (200 male and female participants, 18 years of age or older and with an HbA1c ≥ 8%, will be randomized into one of two groups: (1 an intervention arm employing the innovative FORA system coupled with nurse case management or (2 a usual care group. Participants will be followed for 6-months to ascertain the effect of the interventions on glycemic control. Our primary hypothesis is that among indigent, rural adult patients with T2DM treated in FQHC's, participants randomized to the technology-assisted case management intervention will have significantly greater reduction in HbA1c at 6 months of follow-up compared to usual care. Discussion Results from this study will provide important insight into the effectiveness of technology-assisted case management intervention (TACM for optimizing diabetes care in indigent, rural adult patients with T2DM treated in FQHC's. Trial Registration National Institutes of Health Clinical Trials Registry (http://ClinicalTrials.gov identifier# NCT01373489

  2. Detecting benzoyl peroxide in wheat flour by line-scan macro-scale Raman chemical imaging

    Science.gov (United States)

    Qin, Jianwei; Kim, Moon S.; Chao, Kuanglin; Gonzalez, Maria; Cho, Byoung-Kwan

    2017-05-01

    Excessive use of benzoyl peroxide (BPO, a bleaching agent) in wheat flour can destroy flour nutrients and cause diseases to consumers. A macro-scale Raman chemical imaging method was developed for direct detection of BPO mixed in the wheat flour. A 785 nm line laser was used in a line-scan Hyperspectral Raman imaging system. Raman images were collected from wheat flour mixed with BPO at eight concentrations (w/w) from 50 to 6,400 ppm. A sample holder (150×100×2 mm3) was used to present a thin layer (2 mm thick) of the powdered sample for image acquisition. A baseline correction method was used to correct the fluctuating fluorescence signals from the wheat flour. To isolate BPO particles from the flour background, a simple thresholding method was applied to the single-band fluorescence-free images at a unique Raman peak wavenumber (i.e., 1001 cm-1) preselected for the BPO detection. Chemical images were created to detect and map the BPO particles. Limit of detection for the BPO was estimated in the order of 50 ppm, which is on the same level with regulatory standards.

  3. Linking microstructural evolution and macro-scale friction behavior in metals

    Science.gov (United States)

    Argibay, N.; Chandross, M.; Cheng, S.; Michael, J. R.

    2017-03-01

    A correlation is established between the macro-scale friction regimes of metals and a transition between two dominant atomistic mechanisms of deformation. Metals tend to exhibit bi-stable friction behavior -- low and converging or high and diverging. These general trends in behavior are shown to be largely explained using a simplified model based on grain size evolution, as a function of contact stress and temperature, and are demonstrated for pure copper and gold. Specifically, the low friction regime is linked to the formation of ultra-nanocrystalline surface films (10 to 20 nm), driving toward shear accommodation by grain boundary sliding. Above a critical combination of stress and temperature -- demonstrated to be a material property -- shear accommodation transitions to dislocation dominated plasticity and high friction. We utilize a combination of experimental and computational methods to develop and validate the proposed structure-property relationship. This quantitative framework provides a shift from phenomenological to mechanistic and predictive fundamental understanding of friction for crystalline materials, including engineering alloys.

  4. Reservoir in Global Water Cycle: Macro Scale Hydrologic Modeling for Water Management

    Science.gov (United States)

    Zhou, T.; Nijssen, B.; Haddeland, I.; Gao, H.; Lettenmaier, D. P.

    2014-12-01

    Man-made reservoirs play a key role in the terrestrial water system. They support purposes, such as irrigation, hydropower generation, and flood control, which can substantially change water fluxes at the land surface and redistribute the storage of surface water in space and time. Although most developed countries have sophisticated observing systems for many variables in the natural surface water cycle, long-term and consistent records that focus on water management and human impacts on the global water cycle are much more limited, and most land surface models ignore water management activities. We describe a continental-scale model of reservoir storage, which is combined with a soil moisture deficit-based irrigation scheme within the Variable Infiltration Capacity (VIC) macro-scale hydrological model to simulate the effects of water management in the major river basins of the world. The model is forced with merged NCEP/NCAR and satellite meteorological data at a spatial resolution of 0.25 degrees latitude-longitude, for the period 1948 to 2010. A total of 167 of the largest reservoirs in the world with a total storage capacity around 3900 km3 (nearly 60% of the global total reservoir storage) are simulated. We successfully predict the monthly reservoir storage time series for most of a set of 23 global reservoirs for which observed storage is available either via in situ or satellite remote sensing measurements. We evaluate, on a continental and global basis, the magnitude of inter-seasonal and inter-annual reservoir storage variations in comparison with other terms in the land surface water cycle, including Snow Water Equivalent (SWE) and soil moisture.

  5. Macro-Scale Correction of Precipitation Undercatch in the Midwest/Great Lakes Region

    Science.gov (United States)

    Chiu, C. M.; Hamlet, A. F.

    2015-12-01

    Precipitation gauge undercatch is a serious problem in the context of using observed meteorological data sets for hydrologic modeling studies in regions with cold winters, such as the Midwest. Attention to this matter is urgently needed to support hydroclimatological research efforts in the region. To support hydrologic modeling studies, a new hybrid gridded meteorological dataset at 1/16 degree resolution based on data from CO-OP station records, the U. S. Historical Climatology Network, the Historical Canadian Climate Database, and Precipitation Regression on Independent Slopes Method has been assembled over the Great Lakes and Midwest regions from 1915-2013 at daily time step. Preliminary hydrologic simulations results using the Variable Infiltration Capacity hydrology model with this hybrid gridded meteorological dataset showed that precipitation gauge undercatch was a very significant issue throughout the region, especially for winter snowfall and simulated streamflow, which were both grossly underpredicted. Correction of primary CO-OP station data is generally infeasible due to missing station meta data and lack of local-scale wind speed measurements. Instead, macro-scale post processing techniques were developed to adjust the regridded precipitation product from CO-OP station records from 1950-2013 forwards, accounting for undercatch as a function of regridded wind speed simulations obtained from NCAR Reanalysis. Comparisons of simulated and observed streamflow over seven river basins in the Midwest were used to evaluate the datasets constructed using different combinations of meteorological station inputs, with and without undercatch corrections. The comparisons show promise in producing corrected precipitation data sets from 1950-2013 for hydrologic modeling studies, with substantial improvements in streamflow simulation from the uncalibrated VIC model when gauge undercatch corrections are included.

  6. Quantum manifestation of systems on the macro-scale – the concept of transition state and transition amplitude wave

    Indian Academy of Sciences (India)

    Ram K Varma

    2007-06-01

    Quantum effects which have usually been associated with micro-scale phenomena can also arise on the macro-scale in situations other than the well-known macro-quantum phenomena of superconductivity and superfluidity. Such situations have been shown here to arise in processes involving inelastic scattering with bound or partially bound systems (not bound in all degrees of freedom), and the macro-quantum behaviour is associated with the state of the total system in transition in the process of scattering. Such a state is designated as a `transition-state'. It is pointed out that we have already observed such manifestations for a particular system, the charged particles in a magnetic field where interference effects involving macro-scale matter waves along the magnetic field have been reported [R K Varma et al, Phys. Rev. E65, 026503 (2002)].

  7. Implementation and adaptation of a macro-scale methodology to calculate direct economic losses

    Science.gov (United States)

    Natho, Stephanie; Thieken, Annegret

    2017-04-01

    forestry sector. Furthermore overheads are proposed to include costs of housing content as well as the overall costs of public infrastructure, one of the most important damage sectors. All constants considering sector specific mean sizes or construction costs were adapted. Loss ratios were adapted for each event. Whereas the original UNISDR method over- und underestimates the losses of the tested events, the adapted method is able to calculate losses in good accordance for river floods, hail storms and storms. For example, for the 2013-flood economic losses of EUR 6.3 billion were calculated (UNISDR EUR 0.85 billion, documentation EUR 11 billion). For the hail storms in 2013 the calculated EUR 3.6 billion overestimate the documented losses of EUR 2.7 billion less than the original UNISDR approach with EUR 5.2 billion. Only for flash floods, where public infrastructure can account for more than 90% of total losses, the method is absolutely not applicable. The adapted methodology serves as a good starting point for macro-scale loss estimations by accounting for the most important damage sectors. By implementing this approach into damage and event documentation and reporting standards, a consistent monitoring according to the SFDRR could be achieved.

  8. Picometer-Resolution MEMS Segmented DM Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Microelectromechanical systems (MEMS) technology has the potential to create deformable mirrors (DM) with 10^4 actuators that have size, weight, and power...

  9. Picometer-Resolution MEMS Segmented DM Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Microelectromechanical systems (MEMS) technology has the potential to create deformable mirrors (DM) with 10^4 actuators that have size, weight, and power...

  10. Proposal of a Novel Approach to Developing Material Models for Micro-scale Composites Based on Testing and Modeling of Macro-scale Composites

    Energy Technology Data Exchange (ETDEWEB)

    Siranosian, Antranik Antonio [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Schembri, Philip Edward [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Luscher, Darby Jon [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-04-20

    The Los Alamos National Laboratory's Weapon Systems Engineering division's Advanced Engineering Analysis group employs material constitutive models of composites for use in simulations of components and assemblies of interest. Experimental characterization, modeling and prediction of the macro-scale (i.e. continuum) behaviors of these composite materials is generally difficult because they exhibit nonlinear behaviors on the meso- (e.g. micro-) and macro-scales. Furthermore, it can be difficult to measure and model the mechanical responses of the individual constituents and constituent interactions in the composites of interest. Current efforts to model such composite materials rely on semi-empirical models in which meso-scale properties are inferred from continuum level testing and modeling. The proposed approach involves removing the difficulties of interrogating and characterizing micro-scale behaviors by scaling-up the problem to work with macro-scale composites, with the intention of developing testing and modeling capabilities that will be applicable to the mesoscale. This approach assumes that the physical mechanisms governing the responses of the composites on the meso-scale are reproducible on the macro-scale. Working on the macro-scale simplifies the quantification of composite constituents and constituent interactions so that efforts can be focused on developing material models and the testing techniques needed for calibration and validation. Other benefits to working with macro-scale composites include the ability to engineer and manufacture—potentially using additive manufacturing techniques—composites that will support the application of advanced measurement techniques such as digital volume correlation and three-dimensional computed tomography imaging, which would aid in observing and quantifying complex behaviors that are exhibited in the macro-scale composites of interest. Ultimately, the goal of this new approach is to develop a meso

  11. Preface Gi4DM

    NARCIS (Netherlands)

    Zlatanova, S.; Sithole, G.; Nakagawa, M.; Zhu, Q.

    2015-01-01

    Gi4DM 2015 marks the 10th edition of the Geoinformation for Disaster Management series of conferences. The first conference in 2005 was held in the aftermath of the 2004 Indian Ocean earthquake and tsunami which claimed the lives of over 220000 civilians. While Geoinformatics has a long been used in

  12. Semi-automated image processing system for micro- to macro-scale analysis of immunohistopathology: application to ischemic brain tissue.

    Science.gov (United States)

    Wu, Chunyan; Zhao, Weizhao; Lin, Baowan; Ginsberg, Myron D

    2005-04-01

    Immunochemical staining techniques are commonly used to assess neuronal, astrocytic and microglial alterations in experimental neuroscience research, and in particular, are applied to tissues from animals subjected to ischemic stroke. Immunoreactivity of brain sections can be measured from digitized immunohistology slides so that quantitative assessment can be carried out by computer-assisted analysis. Conventional methods of analyzing immunohistology are based on image classification techniques applied to a specific anatomic location at high magnification. Such micro-scale localized image analysis limits one for further correlative studies with other imaging modalities on whole brain sections, which are of particular interest in experimental stroke research. This report presents a semi-automated image analysis method that performs convolution-based image classification on micro-scale images, extracts numerical data representing positive immunoreactivity from the processed micro-scale images and creates a corresponding quantitative macro-scale image. The present method utilizes several image-processing techniques to cope with variances in intensity distribution, as well as artifacts caused by light scattering or heterogeneity of antigen expression, which are commonly encountered in immunohistology. Micro-scale images are composed by a tiling function in a mosaic manner. Image classification is accomplished by the K-means clustering method at the relatively low-magnification micro-scale level in order to increase computation efficiency. The quantitative macro-scale image is suitable for correlative analysis with other imaging modalities. This method was applied to different immunostaining antibodies, such as endothelial barrier antigen (EBA), lectin, and glial fibrillary acidic protein (GFAP), on histology slides from animals subjected to middle cerebral artery occlusion by the intraluminal suture method. Reliability tests show that the results obtained from

  13. Dse ign and implementation of network video transmission based on DM3730 and GStreamer technology%基于 DM3730和 GStreamer的网络视频传输技术设计与实现

    Institute of Scientific and Technical Information of China (English)

    王洪伟

    2014-01-01

    提出一种低带宽通用的网络传输视频数据的方法,该方法的硬件采用德州仪器ARM+DSP双核的视频处理平台;软件使用基于流水线方式的多媒体框架GStreamer技术,实现了视频采集、H.264压缩与网络传输的功能;最后通过服务器将视频显示出来,服务器在Linux操作系统和Windows XP 操作系统下都可以将摄像头采集的视频信息播放出来,达到远程传输实时监控的效果。%A general low bandwidth network transmission video data method was put forward in this paper .The hard-ware of the method is based on the platform of Texas instruments with dual core of ARM+DSP .The software em-ploys multimedia framework GStreamer technology based on the assembly line way , achieving the functions of video acquisition, H.264 compression and network transmission , and displaying video images by server .The video infor-mation collected by camera can be displayed by server on both Linux and Windows XP OS , achieving the goal of remote transmission and real-time monitoring.

  14. Ubiquitous CM and DM

    Science.gov (United States)

    Crowley, Sandra L.

    2000-01-01

    Ubiquitous is a real word. I thank a former Total Quality Coach for my first exposure some years ago to its existence. My version of Webster's dictionary defines ubiquitous as "present, or seeming to be present, everywhere at the same time; omnipresent." While I believe that God is omnipresent, I have come to discover that CM and DM are present everywhere. Oh, yes; I define CM as Configuration Management and DM as either Data or Document Management. Ten years ago, I had my first introduction to the CM world. I had an opportunity to do CM for the Space Station effort at the NASA Lewis Research Center. I learned that CM was a discipline that had four areas of focus: identification, control, status accounting, and verification. I was certified as a CMIl graduate and was indoctrinated about clear, concise, and valid. Off I went into a world of entirely new experiences. I was exposed to change requests and change boards first hand. I also learned about implementation of changes, and then of technical and CM requirements.

  15. Impact of climate change on flood characteristics in Brahmaputra basin using a macro-scale distributed hydrological model

    Indian Academy of Sciences (India)

    Shyamal Ghosh; Subashisa Dutta

    2012-06-01

    Being the highest specific discharge river system in the world, the Brahmaputra river experiences a number of long-duration flood waves during the monsoon season annually. In order to assess the flood characteristics at the basin and tributary scales, a physically based macro-scale distributed hydrological model (DHM) has been calibrated and validated for 9 wet years. The model performance has been evaluated in terms of prediction of the flood characteristics such as peak discharge, flood duration, arrival time of flood wave, timing of the peak flow and number of flood waves per season. Future changes in the flood wave characteristics of the basin have been evaluated using the validated model with bias-corrected future-projected meteorological scenario from a regional climate model (RCM). Likelihood analysis of the simulated flow time series reveals that significant increase in both peak discharge and flood duration is expected for both the pre-monsoonal and monsoonal seasons in the basin, but the number of flood waves per season would be reduced. Under the projected climate change scenario, it is expected that there will be more catastrophic floods in the basin.

  16. Three-dimensional macro-scale assessment of regional and temporal wall shear stress characteristics on aortic valve leaflets.

    Science.gov (United States)

    Cao, K; Bukač, M; Sucosky, P

    2016-01-01

    The aortic valve (AV) achieves unidirectional blood flow between the left ventricle and the aorta. Although hemodynamic stresses have been shown to regulate valvular biology, the native wall shear stress (WSS) experienced by AV leaflets remains largely unknown. The objective of this study was to quantify computationally the macro-scale leaflet WSS environment using fluid-structure interaction modeling. An arbitrary Lagrangian-Eulerian approach was implemented to predict valvular flow and leaflet dynamics in a three-dimensional AV geometry subjected to physiologic transvalvular pressure. Local WSS characteristics were quantified in terms of temporal shear magnitude (TSM), oscillatory shear index (OSI) and temporal shear gradient (TSG). The dominant radial WSS predicted on the leaflets exhibited high amplitude and unidirectionality on the ventricularis (TSM>7.50 dyn/cm(2), OSI 325.54 dyn/cm(2) s) but low amplitude and bidirectionality on the fibrosa (TSM 0.38, TSG 0.25). This study provides new insights into the role played by leaflet-blood flow interactions in valvular function and critical hemodynamic stress data for the assessment of the hemodynamic theory of AV disease.

  17. Detecting and modelling structures on the micro and the macro scales: Assessing their effects on solute transport behaviour

    Science.gov (United States)

    Haslauer, C. P.; Bárdossy, A.; Sudicky, E. A.

    2017-09-01

    This paper demonstrates quantitative reasoning to separate the dataset of spatially distributed variables into different entities and subsequently characterize their geostatistical properties, properly. The main contribution of the paper is a statistical based algorithm that matches the manual distinction results. This algorithm is based on measured data and is generally applicable. In this paper, it is successfully applied at two datasets of saturated hydraulic conductivity (K) measured at the Borden (Canada) and the Lauswiesen (Germany) aquifers. The boundary layer was successfully delineated at Borden despite its only mild heterogeneity and only small statistical differences between the divided units. The methods are verified with the more heterogeneous Lauswiesen aquifer K data-set, where a boundary layer has previously been delineated. The effects of the macro- and the microstructure on solute transport behaviour are evaluated using numerical solute tracer experiments. Within the microscale structure, both Gaussian and non-Gaussian models of spatial dependence of K are evaluated. The effects of heterogeneity both on the macro- and the microscale are analysed using numerical tracer experiments based on four scenarios: including or not including the macroscale structures and optimally fitting a Gaussian or a non-Gaussian model for the spatial dependence in the micro-structure. The paper shows that both micro- and macro-scale structures are important, as in each of the four possible geostatistical scenarios solute transport behaviour differs meaningfully.

  18. Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels.

    Science.gov (United States)

    Rodriguez-Rivera, Veronica; Weidner, John W; Yost, Michael J

    2016-02-12

    Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper composition, targeted modulus, and well-defined architectural features. Biomaterials that recapitulate the intrinsic architecture of in vivo tissue are vital for studying diseases as well as to facilitate the regeneration of lost and malformed soft tissue. A novel biofabrication technique was developed which combines state of the art imaging, three-dimensional (3D) printing, and selective enzymatic activity to create a new generation of biomaterials for research and clinical application. The developed material, Bovine Serum Albumin rubber, is reaction injected into a mold that upholds specific geometrical features. This sacrificial material allows the adequate transfer of architectural features to a natural scaffold material. The prototype consists of a 3D collagen scaffold with 4 and 3 mm channels that represent a branched architecture. This paper emphasizes the use of this biofabrication technique for the generation of natural constructs. This protocol utilizes a computer-aided software (CAD) to manufacture a solid mold which will be reaction injected with BSA rubber followed by the enzymatic digestion of the rubber, leaving its architectural features within the scaffold material.

  19. Monitoring and assessment of soil erosion at micro-scale and macro-scale in forests affected by fire damage in northern Iran.

    Science.gov (United States)

    Akbarzadeh, Ali; Ghorbani-Dashtaki, Shoja; Naderi-Khorasgani, Mehdi; Kerry, Ruth; Taghizadeh-Mehrjardi, Ruhollah

    2016-12-01

    Understanding the occurrence of erosion processes at large scales is very difficult without studying them at small scales. In this study, soil erosion parameters were investigated at micro-scale and macro-scale in forests in northern Iran. Surface erosion and some vegetation attributes were measured at the watershed scale in 30 parcels of land which were separated into 15 fire-affected (burned) forests and 15 original (unburned) forests adjacent to the burned sites. The soil erodibility factor and splash erosion were also determined at the micro-plot scale within each burned and unburned site. Furthermore, soil sampling and infiltration studies were carried out at 80 other sites, as well as the 30 burned and unburned sites, (a total of 110 points) to create a map of the soil erodibility factor at the regional scale. Maps of topography, rainfall, and cover-management were also determined for the study area. The maps of erosion risk and erosion risk potential were finally prepared for the study area using the Revised Universal Soil Loss Equation (RUSLE) procedure. Results indicated that destruction of the protective cover of forested areas by fire had significant effects on splash erosion and the soil erodibility factor at the micro-plot scale and also on surface erosion, erosion risk, and erosion risk potential at the watershed scale. Moreover, the results showed that correlation coefficients between different variables at the micro-plot and watershed scales were positive and significant. Finally, assessment and monitoring of the erosion maps at the regional scale showed that the central and western parts of the study area were more susceptible to erosion compared with the western regions due to more intense crop-management, greater soil erodibility, and more rainfall. The relationships between erosion parameters and the most important vegetation attributes were also used to provide models with equations that were specific to the study region. The results of this

  20. An Assessment of macro-scale in situ Raman and ultraviolet-induced fluorescence spectroscopy for rapid characterization of frozen peat and ground ice

    Science.gov (United States)

    Laing, Janelle R.; Robichaud, Hailey C.; Cloutis, Edward A.

    2016-04-01

    The search for life on other planets is an active area of research. Many of the likeliest planetary bodies, such as Europa, Enceladus, and Mars are characterized by cold surface environments and ice-rich terrains. Both Raman and ultraviolet-induced fluorescence (UIF) spectroscopies have been proposed as promising tools for the detection of various kinds of bioindicators in these environments. We examined whether macro-scale Raman and UIF spectroscopy could be applied to the analysis of unprocessed terrestrial frozen peat and clear ground ice samples for detection of bioindicators. It was found that this approach did not provide unambiguous detection of bioindicators, likely for a number of reasons, particularly due to strong broadband induced fluorescence. Other contributing factors may include degradation of organic matter in frozen peat to the point that compound-specific emitted fluorescence or Raman peaks were not resolvable. Our study does not downgrade the utility of either UIF or Raman spectroscopy for astrobiological investigations (which has been demonstrated in previous studies), but does suggest that the choice of instrumentation, operational conditions and sample preparation are important factors in ensuring the success of these techniques.

  1. Macro Scale Hydrologic Modeling for Water Management: Re-construction of Large Reservoir Storage Time Series in the Continental U.S

    Science.gov (United States)

    Zhou, T.; Nijssen, B.; Haddeland, I.; Lettenmaier, D. P.

    2013-12-01

    Water management activities such as irrigation water withdrawal, hydropower generation, and flood control, substantially change water fluxes at the land surface and redistribute the storage of surface water in space and time. Although most developed countries have sophisticated observing systems for most variables in the surface water cycle, long-term and consistent records that focus on water management and human impacts on the water cycle are less available. We describe a continental-scale model of reservoir storage, which is combined with a soil moisture deficit-based irrigation scheme within the Variable Infiltration Capacity (VIC) macro scale hydrology model to simulate the effects of water management in the major river basins of the continental U.S. The model is forced with merged NCEP/NCAR and satellite meteorological data at a spatial resolution of 0.5 degrees latitude-longitude, for the period 1948 to 2008. A total of 120 of the largest reservoirs in the U.S. with a storage capacity greater than 1,000,000 acre feet are simulated. Two key variables, time series of monthly irrigation water consumption and monthly reservoir storage, which reflect the water management impacts, are extracted from the model results. The simulation results indicate that the model is able to estimate irrigation water demands successfully in comparison with observations and other inferences, and to accurately re-construct reservoir storage time series. We also discuss early results from global simulations, which allow us to assess human impacts on the global land surface water cycle in data-sparse regions.

  2. Thermodynamics of diffusive DM/DE systems

    Science.gov (United States)

    Haba, Z.

    2017-04-01

    We discuss the energy density, temperature and entropy of dark matter (DM) and dark energy (DE) as functions of the scale factor a in an expanding universe. In a model of non-interacting dark components we repeat a derivation from thermodynamics of the well-known relations between the energy density, entropy and temperature. In particular, the entropy is constant as a consequence of the energy conservation. We consider a model of a DM/DE interaction where the DM energy density increase is proportional to the particle density. In such a model the dependence of the energy density and the temperature on the scale factor a is substantially modified. We discuss (as a realization of the model) DM which consists of relativistic particles diffusing in an environment of DE. The energy gained by the dark matter comes from a cosmological fluid with a negative pressure. We define the entropy and free energy of such a non-equilibrium system. We show that during the universe evolution the entropy of DM is increasing whereas the entropy of DE is decreasing. The total entropy can increase (in spite of the energy conservation) as the DM and DE temperatures are different. We discuss non-equilibrium thermodynamics on the basis of the notion of the relative entropy.

  3. Consequences of DM/antiDM oscillations for asymmetric WIMP darkmatter

    DEFF Research Database (Denmark)

    Cirelli, M.; Panci, P.; Servant, G.

    2012-01-01

    Assuming the existence of a primordial asymmetry in the dark sector, a scenario usually dubbed Asymmetric Dark Matter (aDM), we study the effect of oscillations between dark matter and its antiparticle on the re-equilibration of the initial asymmetry before freeze-out, which enable efficient...... a primordial asymmetry of the same order as the baryon asymmetry naturally gets the correct relic abundance if the DM-number-violating Delta(DM) = 2 mass term is in the similar to meV range. The re-establishment of annihilations implies that constraints from the accumulation of aDM in astrophysical bodies...

  4. Consequences of DM/antiDM oscillations for asymmetric WIMP darkmatter

    DEFF Research Database (Denmark)

    Cirelli, M.; Panci, P.; Servant, G.;

    2012-01-01

    Assuming the existence of a primordial asymmetry in the dark sector, a scenario usually dubbed Asymmetric Dark Matter (aDM), we study the effect of oscillations between dark matter and its antiparticle on the re-equilibration of the initial asymmetry before freeze-out, which enable efficient...... annihilations to recouple. We calculate the evolution of the DM relic abundance and show how oscillations re-open the parameter space of aDM models, in particular in the direction of allowing large (WIMP-scale) DM masses. A typical WIMP with a mass at the EW scale (similar to 100 GeV - 1TeV) presenting...

  5. Partially Melted UHP Eclogite in the Sulu Orogenic Belt, China and its rheological significance to deep continental subduction: Micro- to Macro-scale Evidence

    Science.gov (United States)

    Wang, Lu; Kusky, Timothy; Polat, Ali; Wang, Songjie; Jiang, Xingfu; Zong, Keqing; Wang, Junpeng; Deng, Hao; Fu, Jianmin

    2015-04-01

    Partially Melted UHP Eclogite in the Sulu Orogenic Belt, China and its rheological significance to deep continental subduction: Micro- to Macro-scale Evidence Numerous studies have described partial melting processes in low-high pressure meta-sedimentary rocks, some of which may generate melts that coalesce to form plutons. However, migmatized ultrahigh pressure (UHP) eclogite has never been clearly described from the microscale to macroscale, though experimental studies prove dehydration partial melting of eclogite at high pressure condition1 and low degrees of partially melted eclogite have been reported from the Qaidam UHP orogenic belt in NW China2,3 or inferred from multiphase solid (MS) inclusions within eclogite4 in the Sulu UHP belt. We present field-based documentation of decompression partial melting of UHP eclogite from Yangkou and General's Hill, Sulu Orogen. Migmatized eclogite shows successive stages of anatexis, initially starting from intragranular and grain boundary melt droplets, which grow into a 3D interconnected intergranular network, then segregate and accumulate in pressure shadow areas, and finally merge to form melt channels and dikes that transport melts to upper lithospheric levels. In-situ phengite breakdown-induced partial melting is directly identified by MS inclusions of Kfs+ barium-bearing Kfs + Pl in garnet, connected by 4-10 μm wide veinlets consisting of Bt + Kfs + Pl next to the phengite. Intergranular veinlets of plagioclase + K-feldspar first form isolated beads of melt along grain boundaries and triple junctions of quartz, and with higher degrees of melting, eventually form interconnected 3D networks along grain boundaries in the leucosome, allowing melt to escape from the intergranular realm and collect in low-stress areas. U-Pb (zircon) dating and petrological analyses on residue and leucocratic rocks shows that partial melting occurred at 228-219 Ma, shortly after peak UHP metamorphism (~230 Ma), and at depths of 30-90 km

  6. Influence of pH and Chloride Concentration on the Corrosion Behavior of Unalloyed Copper in NaCl Solution: A Comparative Study Between the Micro and Macro Scales

    Directory of Open Access Journals (Sweden)

    Annemie Adriaens

    2012-11-01

    Full Text Available The effects of pH and chloride concentration on the electrochemical corrosion of copper in aqueous sodium chloride (NaCl media were studied at the micro scale using a microcapillary droplet cell and at the macro scale using a conventional large scale cell. Using an experimental design strategy, electrochemical response surface models of copper versus pH and NaCl concentration were constructed with the minimum number of experiments required. Results show that the electrochemical behavior of copper under corrosive media shows significant differences between the micro and macro scale experiments. At the micro scale, the pit initiation of copper occurs at more negative potentials for high NaCl concentrations and alkaline pH values. Also, the micro scale potentiostatic measurements indicate higher stabilised passive currents at high NaCl concentrations and low (acidic pH values. At the macro scale, the pH is shown to have a greater influence on the corrosion potential. The chloride concentration is the most significant factor in the passive current case while at the micro scale the effect of these two factors on the passive current was found to be the same. The surface morphology of the formed patina on the corroded copper in both micro and macro systems reveal a more significant role of the chloride concentration on the structure and the grain size of the patinas. Finally, micro and macro electrochemical impedance spectroscopy of copper at various NaCl concentrations and pH values demonstrates a different behavior of copper after several potentiodynamic polarization cycles.

  7. TI DM270 PMP解决方案

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ TI PMP特点 TI提供系列PMP解决方案,包括TI DM270/DM275/DM320/DM342/DM420等数字媒体处理器及不同操作系统(linux或wince等)的PMP方案.另外特别值得关注的是微软公司和TI合作推出了PMC(便携式媒体中心)-Ⅱ代.

  8. Identification of 13 DB + dM and 2 DC + dM binaries from the Sloan Digital Sky Survey

    NARCIS (Netherlands)

    Besselaar, E.J.M. van den; Roelofs, G.H.A.; Nelemans, G.A.; Augusteijn, T.; Groot, P.J.

    2005-01-01

    We present the identification of 13 DB + dM binaries and 2 DC + dM binaries from the Sloan Digital Sky Survey (SDSS). Before the SDSS only 2 DB + dM binaries and 1 DC + dM binary were known. At least three, possibly 8, of the new DB + dM binaries seem to have white dwarf temperatures well above 30 0

  9. A Novel Induction Algorithm for DM

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    DM usually means an efficient knowledge discovery from database, and the immune algorithm is a biological theory-based and global searching algorithm. A novel induction algorithm is proposed here which integrates a power of individual immunity and an evolutionary mechanism of population. This algorithm does not take great care of discovering some classifying information, but unknown knowledge or a predication on higher level rules. Theoretical analysis and simulations both show that this algorithm is prone to the stabilization of a population and the improvement of entire capability, and also keeping a high degree of preciseness during the rule induction.

  10. Implementation of SMV based on DM642%基于DM642的SMV实现

    Institute of Scientific and Technical Information of China (English)

    王小兵; 黄冰; 胡巍; 胡斌

    2010-01-01

    介绍了变速率语音算法SMV的DSP实时实现技术.根据标准SMV的要求优化算法的定点C程序,减少占用空间,改善码本的搜索时间,最终在DM642硬件开发平台上运行成功,话音质量符合通信要求.

  11. Human leukocyte Antigen-DM polymorphisms in autoimmune diseases.

    Science.gov (United States)

    Alvaro-Benito, Miguel; Morrison, Eliot; Wieczorek, Marek; Sticht, Jana; Freund, Christian

    2016-08-01

    Classical MHC class II (MHCII) proteins present peptides for CD4(+) T-cell surveillance and are by far the most prominent risk factor for a number of autoimmune disorders. To date, many studies have shown that this link between particular MHCII alleles and disease depends on the MHCII's particular ability to bind and present certain peptides in specific physiological contexts. However, less attention has been paid to the non-classical MHCII molecule human leucocyte antigen-DM, which catalyses peptide exchange on classical MHCII proteins acting as a peptide editor. DM function impacts the presentation of both antigenic peptides in the periphery and key self-peptides during T-cell development in the thymus. In this way, DM activity directly influences the response to pathogens, as well as mechanisms of self-tolerance acquisition. While decreased DM editing of particular MHCII proteins has been proposed to be related to autoimmune disorders, no experimental evidence for different DM catalytic properties had been reported until recently. Biochemical and structural investigations, together with new animal models of loss of DM activity, have provided an attractive foundation for identifying different catalytic efficiencies for DM allotypes. Here, we revisit the current knowledge of DM function and discuss how DM function may impart autoimmunity at the organism level.

  12. DM dimensioning for the next generation AO systems : strategies and rules

    Science.gov (United States)

    Costille, A.; Conan, J.-M.

    2007-07-01

    Adaptive optics (AO) provides a real time correction of turbulence and allows to improve the angular resolution of the astronomical telescopes. The design of AO systems and of its key components (deformable mirror (DM), wave-front sensors...) is very important to provide the best performance. The definition rules are well known for 10m class telescopes. However the study of the next generation of telescopes, especially the Extremely Large Telescope (ELT), associated with the development of new technological solutions, particularly concerning DMs, require to refine and in some cases to reconsider these rules. Both turbulent parameters (seeing, outer scale ...), telescope effects, system design and performance requirements have to be considered in order to define the DM key parameters (stroke, inter-actuator stroke, influence function ...). We present a study based on the combination of analytical expressions and numerical simulation results. We do not give absolute rules of design but we present key aspects to keep in mind when designing the DM.

  13. Differences in aberrant expression and splicing of sarcomeric proteins in the myotonic dystrophies DM1 and DM2.

    Science.gov (United States)

    Vihola, Anna; Bachinski, Linda L; Sirito, Mario; Olufemi, Shodimu-Emmanuel; Hajibashi, Shohrae; Baggerly, Keith A; Raheem, Olayinka; Haapasalo, Hannu; Suominen, Tiina; Holmlund-Hampf, Jeanette; Paetau, Anders; Cardani, Rosanna; Meola, Giovanni; Kalimo, Hannu; Edström, Lars; Krahe, Ralf; Udd, Bjarne

    2010-04-01

    Aberrant transcription and mRNA processing of multiple genes due to RNA-mediated toxic gain-of-function has been suggested to cause the complex phenotype in myotonic dystrophies type 1 and 2 (DM1 and DM2). However, the molecular basis of muscle weakness and wasting and the different pattern of muscle involvement in DM1 and DM2 are not well understood. We have analyzed the mRNA expression of genes encoding muscle-specific proteins and transcription factors by microarray profiling and studied selected genes for abnormal splicing. A subset of the abnormally regulated genes was further analyzed at the protein level. TNNT3 and LDB3 showed abnormal splicing with significant differences in proportions between DM2 and DM1. The differential abnormal splicing patterns for TNNT3 and LDB3 appeared more pronounced in DM2 relative to DM1 and are among the first molecular differences reported between the two diseases. In addition to these specific differences, the majority of the analyzed genes showed an overall increased expression at the mRNA level. In particular, there was a more global abnormality of all different myosin isoforms in both DM1 and DM2 with increased transcript levels and a differential pattern of protein expression. Atrophic fibers in DM2 patients expressed only the fast myosin isoform, while in DM1 patients they co-expressed fast and slow isoforms. However, there was no increase of total myosin protein levels, suggesting that aberrant protein translation and/or turnover may also be involved.

  14. Differences in aberrant expression and splicing of sarcomeric proteins in the myotonic dystrophies DM1 and DM2

    OpenAIRE

    2010-01-01

    Aberrant transcription and mRNA processing of multiple genes due to RNA-mediated toxic gain-of-function has been suggested to cause the complex phenotype in myotonic dystrophies type 1 and 2 (DM1 and DM2). However, the molecular basis of muscle weakness and wasting and the different pattern of muscle involvement in DM1 and DM2 are not well understood. We have analyzed the mRNA expression of genes encoding muscle-specific proteins and transcription factors by microarray profiling and studied s...

  15. EW scale DM models with dark gauge symmetries

    CERN Document Server

    Ko, P

    2016-01-01

    In this talk, I describe a class of electroweak (EW) scale dark matter (DM) models where its stability or longevity are the results of underlying dark gauge symmetries: stable due to unbroken local dark gauge symmetry or topology, or long-lived due to the accidental global symmetry of dark gauge theories. Compared with the usual phenomenological dark matter models (including DM EFT or simplified DM models), DM models with local dark gauge symmetries include dark gauge bosons, dark Higgs bosons and sometimes excited dark matter. And dynamics among these fields are completely fixed by local gauge principle. The idea of singlet portals including the Higgs portal can thermalize these hidden sector dark matter very efficiently, so that these DM could be easily thermal DM. I also discuss the limitation of the usual DM effective field theory or simplified DM models without the full SM gauge symmetry, and emphasize the importance of the full SM gauge symmetry and renormalizability especially for collider searches for...

  16. Macro-scale turbulence modelling for flows in porous media; Modelisation a l'echelle macroscopique d'un ecoulement turbulent au sein d'un milieu poreux

    Energy Technology Data Exchange (ETDEWEB)

    Pinson, F

    2006-03-15

    - This work deals with the macroscopic modeling of turbulence in porous media. It concerns heat exchangers, nuclear reactors as well as urban flows, etc. The objective of this study is to describe in an homogenized way, by the mean of a spatial average operator, turbulent flows in a solid matrix. In addition to this first operator, the use of a statistical average operator permits to handle the pseudo-aleatory character of turbulence. The successive application of both operators allows us to derive the balance equations of the kind of flows under study. Two major issues are then highlighted, the modeling of dispersion induced by the solid matrix and the turbulence modeling at a macroscopic scale (Reynolds tensor and turbulent dispersion). To this aim, we lean on the local modeling of turbulence and more precisely on the k - {epsilon} RANS models. The methodology of dispersion study, derived thanks to the volume averaging theory, is extended to turbulent flows. Its application includes the simulation, at a microscopic scale, of turbulent flows within a representative elementary volume of the porous media. Applied to channel flows, this analysis shows that even within the turbulent regime, dispersion remains one of the dominating phenomena within the macro-scale modeling framework. A two-scale analysis of the flow allows us to understand the dominating role of the drag force in the kinetic energy transfers between scales. Transfers between the mean part and the turbulent part of the flow are formally derived. This description significantly improves our understanding of the issue of macroscopic modeling of turbulence and leads us to define the sub-filter production and the wake dissipation. A f - <{epsilon}>f - <{epsilon}{sub w}>f model is derived. It is based on three balance equations for the turbulent kinetic energy, the viscous dissipation and the wake dissipation. Furthermore, a dynamical predictor for the friction coefficient is proposed. This model is then

  17. Konfirmasi spesifitas GAD65 terhadap anti-GAD65 pada tikus DM dan pasien DM tipe 1

    Directory of Open Access Journals (Sweden)

    Aulanni’a Aulanni’a

    2012-02-01

    Full Text Available The use of glutamic acid decarboxylase (GAD65 from bovine brain has been studied to obtain basic knowledge and diagnosis and prediction of Type 1 Diabetes Mellitus (DM patients. The importance of GAD65 in DM diagnosis based on its patogenesis. One of the autoimmune marker that can be used to detect beta-pancreas destruction in Diabetes Type I is the antibody to glutamic acid decarboxylase (GAD65. Most of the pre-diabetic patients indicate the reactive autoantibody to GAD65. For early detection of anti-GAD65 in the serum of the patient, human recombinat GAD65 has been succeed to be used. However this is not economical, therefore, it is necessary to find the alternative source of cheaper GAD65. The aim of this research is to develop an early detection kit of Type 1 DM based on antibody- GAD65, since the longest patient suffering from DM has higher probability to be complicated, especially for uncured patients. The anti- GAD65 antibodies induced by anti-GAD65 synthetized and labelled by alkaline phosphatase can be used as reagent detection early DM patients. The ten patients of DM as samples (positive of anti-GAD65 and five rats of DM were positive with western blott technique using reagents as result of this research. It can be concluded, GAD65 enzyme isolated from bovine brain induced anti-GAD65 production and have possibilities to be packaged in a diagnostic kit for patient pre DM.

  18. DM-Ice: Current Status and Future Prospects

    CERN Document Server

    Pettus, Walter C

    2015-01-01

    DM-Ice is a program towards the first direct detection search for dark matter in the Southern Hemisphere with a 250 kg-scale NaI(Tl) crystal array. It will provide a definitive understanding of the modulation signal reported by DAMA by running an array at both Northern and Southern Hemisphere sites. A 17 kg predecessor, DM-Ice17, was deployed in December 2010 at a depth of 2457 m under the ice at the geographic South Pole and has concluded its 3.5 yr data run. An active R&D program is underway to investigate detectors with lower backgrounds and improved readout electronics; two crystals with 37 kg combined mass are currently operating at the Boulby Underground Laboratory. We report on the final analyses of the DM-Ice17 data and describe progress towards a 250 kg DM-Ice experiment.

  19. Development of three-dimensional memory (3D-M)

    Science.gov (United States)

    Yu, Hong-Yu; Shen, Chen; Jiang, Lingli; Dong, Bin; Zhang, Guobiao

    2016-10-01

    Since the invention of 3-D ROM in 1996, three-dimensional memory (3D-M) has been under development for nearly two decades. In this presentation, we'll review the 3D-M history and compare different 3D-Ms (including 3D-OTP from Matrix Semiconductor, 3D-NAND from Samsung and 3D-XPoint from Intel/Micron).

  20. Optimization strategy of communication performance in 2D macro-scale pseudo-particle parallel simulation%二维宏观拟颗粒并行模拟程序通信性能优化策略

    Institute of Scientific and Technical Information of China (English)

    严历; 郭力

    2009-01-01

    As the application of particle simulation parallel computing becomes more and more widely used, the communication per-formances in the particle simulation parallel programs can significantly affect the simulation performances when the parallel scale increa-ses, which even may become the major performance bottleneck of the simulation. Based on the analysis of the factors that may affect the communication performances in parallel programs, optimization tests on process partitioning and communication protocol for the commu-nication performance in a 2D macro-scale pseudo-particle parallel simulation program, a typical particle simulation parallel program have been done on Gigabit Etheroet. With improved process partitioning and use of user-level communication protocol, communication performance of the testing program has been enhanced. Some communication performance optimization strategies for particle simulation parallel programs are also proposed in the paper.%随着粒子模拟并行计算在相关领域应用的不断深入和并行节点计算能力的不断增强,粒子模拟并行程序中通信耗时对整体性能的影响越来越显著,甚至成为主要性能瓶颈.本文在分析影响并行程序通信性能多种因素的基础上,从进程划分方式选择、通信协议优化的角度,对1个典型粒子模拟并行程序——二维宏观拟颗粒并行模拟程序在千兆以太网环境下的通信性能的优化策略进行了测试研究,通过改进并行进程划分方式,采用用户级通信协议等方法,使测试程序通信性能获得明显提高,进而提出了粒子模拟并行程序通信性能优化的思路和建议.

  1. PERBEDAAN POLA KONSUMSI DAN STATUS GIZI ANTARA REMAJA DENGAN ORANG TUA DIABETES MELITUS (DM) DAN NON DM

    OpenAIRE

    Marine, Denov; Adiningsih, Sri

    2017-01-01

    Adolescents prefer to consume high fat and high sugar food compare to healthy food such as fruit and vegetable. High fat and high sugar food are risk factor of diabetes mellitus development, especially in adolescents whose parents had diabetes mellitus. The aim of this study was to analyze the difference of eating habit and nutritional status between adolescents with DM parents and non DM parents. This was a cross-sectional research included 42 adolescents as sample that divided into 21 adole...

  2. File list: Oth.ALL.10.dm.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.10.dm.AllCell dm3 TFs and others dm All cell types SRX160967,SRX160968,SRX3...35496,SRX495787,SRX495790,SRX495786,SRX335495 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.ALL.10.dm.AllCell.bed ...

  3. File list: Oth.CeL.50.dm.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CeL.50.dm.AllCell dm3 TFs and others dm Cell line SRX160968,SRX160967,SRX495786...,SRX495787,SRX335495,SRX335496,SRX495790 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.CeL.50.dm.AllCell.bed ...

  4. File list: Oth.CeL.05.dm.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CeL.05.dm.AllCell dm3 TFs and others dm Cell line SRX160967,SRX495786,SRX160968...,SRX495790,SRX335495,SRX335496,SRX495787 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.CeL.05.dm.AllCell.bed ...

  5. File list: Oth.CeL.10.dm.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CeL.10.dm.AllCell dm3 TFs and others dm Cell line SRX160967,SRX160968,SRX335496...,SRX495787,SRX495790,SRX495786,SRX335495 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.CeL.10.dm.AllCell.bed ...

  6. Design Overview of the DM Radio Pathfinder Experiment

    CERN Document Server

    Silva-Feaver, Maximiliano; Cho, Hsiao-Mei; Dawson, Carl; Graham, Peter; Irwin, Kent; Kuenstner, Stephen; Li, Dale; Mardon, Jeremy; Moseley, Harvey; Mule, Richard; Phipps, Arran; Rajendran, Surjeet; Steffen, Zach; Young, Betty

    2016-01-01

    We introduce the DM Radio, a dual search for axion and hidden photon dark matter using a tunable superconducting lumped-element resonator. We discuss the prototype DM Radio Pathfinder experiment, which will probe hidden photons in the 500 peV (100 kHz)-50 neV (10 MHz) mass range. We detail the design of the various components: the LC resonant detector, the resonant frequency tuning procedure, the differential SQUID readout circuit, the shielding, and the cryogenic mounting structure. We present the current status of the pathfinder experiment and illustrate its potential science reach in the context of the larger experimental program.

  7. Electroweak supersymmetric dark matter annihilation in DM rate at NLO

    Energy Technology Data Exchange (ETDEWEB)

    Schmiemann, Saskia; Klasen, Michael; Kovarik, Karol; Steppeler, Patrick [Institut fuer Theoretische Physik, Universitaet Muenster (Germany); Herrmann, Bjoern [LAPTh, Universite Savoie Mont Blanc, CNRS (France); Harz, Julia [CNRS, UMR 7589, LPTHE, Paris (France); Sorbonne Universites, Institut Lagrange de Paris (ILP) (France); Sorbonne Universites, UPMC Univ Paris 06, UMR 7589, LPTHE (France)

    2016-07-01

    Today there are several pieces of evidence for dark matter. One well-known experiment is the measurement of the Dark Matter relic density by the Planck satellite. The talk introduces the 'Dark Matter at next-to-leading order' (DM rate at NLO) project which provides predictions for the dark matter relic density in the MSSM including higher-order corrections. After an introduction of the project DM rate at NLO, I shortly speak about the calculation of the electroweak processes. The main focus lies on the effects of the electroweak tree-level processes on the relic density of neutralinos within selected scenarios.

  8. Design Overview of the DM Radio Pathfinder Experiment

    OpenAIRE

    Silva-Feaver, Maximiliano; Chaudhuri, Saptarshi; Cho, Hsiao-Mei; Dawson, Carl; Graham, Peter; Irwin, Kent; Kuenstner, Stephen; Li, Dale; Mardon, Jeremy; Moseley, Harvey; Mule, Richard; Phipps, Arran; Rajendran, Surjeet; Steffen, Zach; Young, Betty

    2016-01-01

    We introduce the DM Radio, a dual search for axion and hidden photon dark matter using a tunable superconducting lumped-element resonator. We discuss the prototype DM Radio Pathfinder experiment, which will probe hidden photons in the 500 peV (100 kHz)-50 neV (10 MHz) mass range. We detail the design of the various components: the LC resonant detector, the resonant frequency tuning procedure, the differential SQUID readout circuit, the shielding, and the cryogenic mounting structure. We prese...

  9. LikeDM: likelihood calculator of dark matter detection

    CERN Document Server

    Huang, Xiaoyuan; Yuan, Qiang

    2016-01-01

    With the large progresses of searching for dark matter (DM) particles from indirect and direct methods, we develop a numerical tool which enables fast calculation of the likelihood of specified DM particle models given a number of observational data, such as charged cosmic rays from space-borne experiments (e.g., PAMELA, AMS-02), $\\gamma$-rays from Fermi space telescope, and the underground direct detection experiments. The purpose of this tool, \\likedm\\ --- likelihood calculator of dark matter detection, is to bridge the particle model of DM and the observational data. The intermediate steps between these two, including the astrophysical backgrounds, the propagation of charged particles, the analysis of Fermi $\\gamma$-ray data, as well as the DM velocity distribution and the nuclear form factor, have been dealt with in the code. We release the first version (v1.0) focusing on the constraints of charged cosmic and gamma rays and the direct detection part will be implemented in the next version. This manual de...

  10. Antigen list: dm3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available dm3 TFs and others GFP SRX016140,SRX016156,SRX016162,SRX016166,SRX016170,SRX025466,SRX025470,SRX025476,...SRX030958,SRX030960,SRX030962,SRX030964,SRX032116,SRX032121,SRX032125,SRX033313,SRX03331...5,SRX041381,SRX320155,SRX320156,SRX661230,SRX661232,SRX737176,SRX750072,SRX750083 ...

  11. Modified DM Models for Aging Networks Based on Neighborhood Connectivity

    Institute of Scientific and Technical Information of China (English)

    WEI Du-Qu; LIN Min; LUO Xiao-Shu; WANG Gang; ZOU Yan-Li; CHEN Tian-Lun

    2008-01-01

    Two modified Dorogovtsev-Mendes (DM) models of aging networks based on the dynamics of connecting nearest-neighbors are introduced. One edge of the new site is connected to the old site with probabilityekt-αas in the DM's model, where the degree and age of the old site are k and t, respectively. We consider two eases, I.e. The other edges of the new site attaching to the nearest-neighbors of the old site with uniform and degree connectivity probability, respectively. The network structure changes with an increase of aging exponent α. It is found that the networks can produce scale-free degree distributions with small-world properties. And the different connectivity probabilities lead to the different properties of the networks.

  12. Hyperuricaemia and the metabolic syndrome in type 2 DM

    Directory of Open Access Journals (Sweden)

    Ogbera Anthonia O

    2010-04-01

    Full Text Available Abstract Background Elevated serum uric acid levels (SUA have been associated with an increased risk of cardiovascular diseases and the metabolic syndrome (MetS and are often reported to be higher in females than in males. The aim of this report is to determine the prevalence and clinical correlates of hyperuricaemia and also to evaluate associations with the MetS in people with type 2 diabetes mellitus (DM. Methods This was a cross-sectional study conducted in people with type 2 DM in Lagos, Nigeria. Hyperuricaemia was defined by cut-off values of > 7 mg/dl for men and > 6 mg/dl for women. The diagnosis of MetS was made using the new definition by the American Heart Association and other related bodies. Clinical and biochemical parameters were compared between subjects with hyperuricaemia and normouricaemia. Statistical analysis included usage of Student's t test, Pearson correlation coefficients, multivariate regression analysis and chi square. Results 601 patients with type 2 DM aged between 34-91 years were recruited for the study. The prevalence rates of hyperuricaemia and the MetS were 25% and 60% respectively. The frequency of occurrence of hyperuricaemia was comparable in both genders (59% vs 41%, p = 0.3. Although, the prevalence of the MetS in subjects with hyperuricaemia and normouricaemia was comparable (61 vs 56%, p = 0.1, a higher proportion of hyperuricaemic subjects had 3 or more components of the Mets compared with normouricaemic subjects. Possible predictors of hyperuricaemia include central obesity, smoking and elevated serum triglycerides (TG. SUA levels were found to be positively and significantly associated with serum TG (r = 0.2, p = 0.0001 and total cholesterol (r = 13, p = 0.001. Conclusion The prevalence of hyperuricaemia in subjects with type 2 DM is comparable in both genders and possible predictors of hyperuricaemia are potentially modifiable. SUA is positively and significantly associated with serum TG and total

  13. In vitro mapping of Myotonic Dystrophy (DM) gene promoter

    Energy Technology Data Exchange (ETDEWEB)

    Storbeck, C.J.; Sabourin, L. [Univ. of Ottawa (Canada); Baird, S. [Children`s Hospital of Eastern Ontario, Ottawa (Canada)] [and others

    1994-09-01

    The Myotonic Dystrophy Kinase (DMK) gene has been cloned and shared homology to serine/threonine protein kinases. Overexpression of this gene in stably transfected mouse myoblasts has been shown to inhibit fusion into myotubes while myoblasts stably transfected with an antisense construct show increased fusion potential. These experiments, along with data showing that the DM gene is highly expressed in muscle have highlighted the possibility of DMK being involved in myogenesis. The promoter region of the DM gene lacks a consensus TATA box and CAAT box, but harbours numerous transcription binding sites. Clones containing extended 5{prime} upstream sequences (UPS) of DMK only weakly drive the reporter gene chloramphenicol acetyl transferase (CAT) when transfected into C2C12 mouse myoblasts. However, four E-boxes are present in the first intron of the DM gene and transient assays show increased expression of the CAT gene when the first intron is present downstream of these 5{prime} UPS in an orientation dependent manner. Comparison between mouse and human sequence reveals that the regions in the first intron where the E-boxes are located are highly conserved. The mapping of the promoter and the importance of the first intron in the control of DMK expression will be presented.

  14. A Forming Pair of Dwarf Galaxies and Its DM Halo

    Science.gov (United States)

    Pustilnik, S. A.; Brinks, E.; Thuan, T. X.; Izotov, Yu.; Lipovetsky, V.

    SBS 0335-052 and its companion 0335-052W are shown to be a unique pair of currently forming dwarf galaxies associated with a huge HI cloud (Izotov et al. 1997, Lipovetsky et al. 1997, Thuan et al. 1997, Pustilnik et al. 1997). We present the analysis of the velocity field of this HI cloud, obtained with the VLA, and the model of its rotation curve to derive the total mass distribution in this system. We argue that this gas cloud is rotationally supported in the gravitational potential of a massive DM halo, and discuss the implications of this fact for galaxy formation and evolution scenarios. This unique system apparently preserves the original unperturbed structure of its DM halo and is thus very valuable to confront halo properties with model predictions. The parameters of the DM halo and neutral gas set limits, based on observational evidence, to the range of physical conditions under which a pristine hydrogen cloud can survive as a stable object over cosmlogical time scales. We discuss the possible effect of the massive spiral galaxy at the projected distance of about 100/h kpc to these forming galaxies.

  15. Immune dysfunction in patients with diabetes mellitus (DM).

    Science.gov (United States)

    Geerlings, S E; Hoepelman, A I

    1999-12-01

    Patients with diabetes mellitus (DM) have infections more often than those without DM. The course of the infections is also more complicated in this patient group. One of the possible causes of this increased prevalence of infections is defects in immunity. Besides some decreased cellular responses in vitro, no disturbances in adaptive immunity in diabetic patients have been described. Different disturbances (low complement factor 4, decreased cytokine response after stimulation) in humoral innate immunity have been described in diabetic patients. However, the clinical relevance of these findings is not clear. Concerning cellular innate immunity most studies show decreased functions (chemotaxis, phagocytosis, killing) of diabetic polymorphonuclear cells and diabetic monocytes/macrophages compared to cells of controls. In general, a better regulation of the DM leads to an improvement of these cellular functions. Furthermore, some microorganisms become more virulent in a high glucose environment. Another mechanism which can lead to the increased prevalence of infections in diabetic patients is an increased adherence of microorganisms to diabetic compared to nondiabetic cells. This has been described for Candida albicans. Possibly the carbohydrate composition of the receptor plays a role in this phenomenon.

  16. Is mortalin a candidate gene for T1DM ?

    Science.gov (United States)

    Johannesen, Jesper; Pie, Angeles; Karlsen, Allan Ertmann; Larsen, Zenia Marian; Jensen, Allan; Vissing, Henrik; Kristiansen, Ole Peter; Pociot, Flemming; Nerup, Jørn

    2004-01-01

    Mortalin has been found to be up-regulated by 2D-protein gel analysis in isolated rodent islets exposed to cytokines. In islets from two rat strains with different sensitivity to the toxic effects of cytokines we observed a significant difference in IL-1beta mediated mortalin expression. Constitutive over-expression of rat mortalin in NIH3T3 cells reduced cellular survival in accordance with mortalin being associated to cellular senescence. Hence we consider the gene encoding for mortalin at chromosome 5q31.1 a putative candidate gene in cytokine induced beta-cell destruction. We scanned the human mortalin gene for polymorphisms and identified three novel polymorphisms. Neither the SNPs individually nor as constructed haplotypes showed disease association tested by (E)TDT in a Danish type 1 diabetes (T1DM) population. Furthermore, we tested the D5S500 microsatelite located close to 5q31.1 without finding linkage to (T1DM). In conclusion, the functional data identifying a difference in mortalin expression in IL-1beta stimulated islets between two rat strains and over-expression of mortalin in NIH3T3 cells associated with decreased viability suggests a functional role for mortalin in cytokine mediated beta cell destruction; however, the identified polymorphisms did not reveal any association in the presence of linkage disequilibrium of mortalin to T1DM in the Danish population.

  17. Perbandingan kadar hs-CRP pada penderita DM tipe 2 yang dislipidemi dan nondislipidemi

    OpenAIRE

    Pasaribu, Juli Y.M

    2016-01-01

    Introduction : DM type 2 is a global health problem which the prevalence is getting more increased. One of the complications DM type 2 is the coronary artery disease (CAD). CAD is caused by the endothelial dysfunction which later becomes atherosclerosis. Besides DM, dyslipidemia might cause the endothelial dysfunction. DM and dyslipidemia, each of them might cause the endothelium dysfunction, furthermore, if it is found that the DM patients suffer dyslipidemia, it will increase the risk of th...

  18. [Characterization of a thermophilic Geobacillus strain DM-2 degrading hydrocarbons].

    Science.gov (United States)

    Liu, Qing-kun; Wang, Jun; Li, Guo-qiang; Ma, Ting; Liang, Feng-lai; Liu, Ru-lin

    2008-12-01

    A thermophilic Geobacillus strain DM-2 from a deep-subsurface oil reservoir was investigated on its capability of degrading crude oil under various conditions as well as its characters on degrading hydrocarbons in optimal conditions. The results showed that Geobacillus strain DM-2 was able to degrade crude oil under anoxic wide-range conditions with pH ranging from 4.0 to 10.0, high temperature in the range of 45-70 degrees C and saline concentration ranging from 0.2% to 3.0%. Furthermore, the optimal temperature and pH value for utilizing hydrocarbons by the strain were 60 degrees C and 7.0, respectively. Under such optimal conditions, the strain utilized liquid paraffine emulsified by itself as its carbon source for growth; further analysis by gas chromatography (GC) and infrared absorption spectroscopy demonstrated that it was able to degrade n-alkanes (C14-C30), branched-chain alkanes and aromatic hydrocarbons in crude oil and could also utilize long-chain n-alkanes from C16 to C36, among of which the degradation efficiency of C28 was the highest, up to 88.95%. One metabolite of the strain oxidizing alkanes is fatty acid.While utilizing C16 as carbon source for 5 d, only one fatty acid-acetic acid was detected by HPLC and MS as the product, with the amount of 0.312 g/L, which indicated that it degraded n-alkanes with pathway of inferior terminal oxidation,and then followed by a beta-oxidation pathway. Due to its characters of efficient emulsification, high-performance degradation of hydrocarbons and fatty-acid production under high temperature and anoxic condition, the strain DM-2 may be potentially applied to oil-waste treatment and microbial enhanced heavy oil recovery in extreme conditions.

  19. Identifying Bank Frauds Using CRISP-DM and Decision Trees

    Directory of Open Access Journals (Sweden)

    Bruno Carneiro da Rocha

    2010-10-01

    Full Text Available This article aims to evaluate the use of techniques of decision trees, in conjunction with the managementmodel CRISP-DM, to help in the prevention of bank fraud. This article offers a study on decision trees, animportant concept in the field of artificial intelligence. The study is focused on discussing how these treesare able to assist in the decision making process of identifying frauds by the analysis of informationregarding bank transactions. This information is captured with the use of techniques and the CRISP-DMmanagement model of data mining in large operational databases logged from internet banktransactions.

  20. Implementing VLPR systems based on TMS320DM642

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This paper gives a practical schema for using DSP boards to construct Vehicle License Plate Recognition (VLPR) modules that could be embedded in any Intelligent Transportation System (ITS). Using DSP can avoid the heavy investment in dedicated VLPR system and improve the computational power compared to PC software environment. Low cost, high computational power, and high flexibility of DSP provide the License Plate Recognition System (LPRS) an excellent cost-effective solution to execute the major part of the recognition tasks. This paper describes a successful implementation of VLPR system based on Texas Instruments (TI)'s TMS320DM642. The DSP board acquires video (which could be output to a monitor for surveillance)from a camera, captures images from the video, locates and recognizes the license plates in images, and then sends the recognized results and related images after compression to a host PC through the network. Finally, the overall software is optimized according to the features of DM642 chip. Experiments showed that the DSP VLPR system performs well on the local license plates, and that the processing speed and accuracy can meet the requirement of practical applications.

  1. Recent advances on macro-scale and micro-scale dynamic interaction between high earth-rock dams and water%高土石坝宏细观坝水动力流固耦合理论研究进展

    Institute of Scientific and Technical Information of China (English)

    岑威钧; 孙辉; 陈亚南

    2013-01-01

    The dynamic interaction will happen among high earth-rock dams, reservoir and pore water under a strong earthquake. This paper introduces the main research history, research results and research trends of water-soil coupling system for earth-rock dams from the macro-scale and micro-scale aspects. On the macro-scale dynamic coupling system, the early dam-water interaction model, sophisticated analytical methods of reservoir water and FSI coordinate system description are summarized, and on the micro-scale dynamic coupling system, mainly from the early water-soil decoupled dynamic analysis method, and the water-soil coupling analysis system based on the Boit's theory and the water-soil mixture theory, ect. Combining the above two research aspects of soil-water coupling system, a macro-scale and micro-scale coupling system of reservoir-dam-pore water is proposed to study the dynamic behavior of high earth-rock dams comprehensively. Finally, the main problems on the macro-scale and micro-scale water-soil coupling theory and further research topics are put forward.%高土石坝遭遇强烈地震时会与坝面库水及坝内孔隙水发生动力流固耦合相互作用,分别从宏观坝水耦合系统和细观水土耦合系统两个角度对高土石坝坝水动力流固耦合理论的研究历史、主要研究成果和研究趋势进行了阐述评价。对宏观尺度的大坝与坝面库水流固耦合作用,主要从早期坝水相互作用模型、库水运动精细分析方法以及流固耦合系统坐标描述等方面做出评述;对细观尺度的坝内水土耦合作用,主要从早期解耦或拟耦合的水土动力分析方法、基于Boit动力固结理论的细观水土动力流固耦合,以及基于混合物理论的细观水土动力流固耦合等方面做出评述。综合两个尺度的流固耦合作用研究现状,建议对高土石坝建立水库土石坝孔隙水的宏细观整体动力流固耦合系统进行综合研究,并

  2. Aberrant intracellular metabolism of T-DM1 confers T-DM1 resistance in HER2-positive gastric cancer cells.

    Science.gov (United States)

    Wang, Hongbin; Wang, Wenqian; Xu, Yongping; Yang, Yong; Chen, Xiaoyan; Quan, Haitian; Lou, Liguang

    2017-04-07

    T-DM1 (Kadcyla), an antibody-drug conjugate (ADC) consisting of HER2-targeted monoclonal antibody trastuzumab linked to anti-microtubule agent mertansine (DM1), has been approved for the treatment of HER2-positive metastatic breast cancer. To date, acquired resistance arises to be a major obstacle to T-DM1 treatment, and mechanisms remain incompletely understood. In the present study, we established a T-DM1-resistant N87-KR cell line from HER2-positive N87 gastric cancer cells to investigate mechanisms of acquired resistance and develop strategies for overcoming it. Although the kinetics of binding, internalization, and externalization of T-DM1 were the same in N87-KR cells and N87 cells, N87-KR was strongly resistant to T-DM1, but remained sensitive to both trastuzumab and DM1. T-DM1 failed to inhibit microtubule polymerization in N87-KR cells. Consistently, lysine-MCC-DM1, the active T-DM1 metabolite that inhibits microtubule polymerization, accumulated much lesser in N87-KR cells. Furthermore, lysosome acidification, achieved by V-ATPase, was much diminished in N87-KR cells. Notably, treatment of sensitive N87 cells with the V-ATPase-selective inhibitor Baf-A1 induced T-DM1 resistance, suggesting that aberrant V-ATPase activity decreases T-DM1 metabolism, leading to T-DM1 resistance in N87-KR cells. Interestingly, HER2-targeted ADCs containing a protease-cleavable linker, such as hertuzumab-vc-MMAE, were capable of efficiently overcoming this resistance. Our results demonstrate for the first time that a decrease in T-DM1 metabolites induced by aberrant V-ATPase activity contributes to T-DM1 resistance, which could be overcome by HER2-targeted ADC containing different linkers, including a protease-cleavable linker. Accordingly, we propose that V-ATPase activity in lysosomes is a novel biomarker for predicting T-DM1 resistance. This article is protected by copyright. All rights reserved.

  3. STT Doubles with Large DM - Part IV: Ophiuchus and Hercules

    Science.gov (United States)

    Knapp, Wilfried; Nanson, John

    2016-04-01

    The results of visual double star observing sessions suggested a pattern for STT doubles with large DM of being harder to resolve than would be expected based on the WDS catalog data. It was felt this might be a problem with expectations on one hand, and on the other might be an indication of a need for new precise measurements, so we decided to take a closer look at a selected sample of STT doubles and do some research. We found that like in the other constellations covered so far (Gem, Leo, UMa, etc.) at least several of the selected objects in Ophiuchus and Hercules show parameters quite different from the current WDS data.

  4. Micro-production using Bits-out technologies

    DEFF Research Database (Denmark)

    Hansen, Hans Nørgaard

    Micro products can be characterised as having at least one critical dimension or functional feature in the sub-mm range. The production of such micro components requires the use of processes usually combined into complex process chains. The choice of processes depends largely on the material...... involved and on the complexity and characteristics of the components. For micro products in metals, polymers or ceramics down-scaling of macro-scale production technologies is a suitable way forward. This however poses challenges in terms of the so-called size effects and also the supporting technologies...

  5. SELF MANAGEMENT INTERVENTION INCREASING COMPLIANCE IN PATIENT WITH DM

    Directory of Open Access Journals (Sweden)

    Siti Nur Kholifah

    2017-01-01

    Full Text Available Introduction: Diabetes mellitus (DM was a degenerative disease which often found in the community. Diabetes was caused by unhealthy habits, such as overeating, lack of exercise, and stress. The purpose of this study was to identify selfmanagement as one of the interventions that can improve treatment compliance in patients with diabetes. Method: This study was used quasy experiments non randomized pretest-posttest design. Samples were 20 families who lived with type 2 diabetes patient. Variable independent was self management intervention and variable dependent was patient complience. Data were collected by using interview, food recall, and observation on behavioral change. Data then analyzed by using paired t-test with α≤0.05. Results: The results had showed that before intervention only 3 (15 % respondents who obey diabetes diet, then increase to 19 (95 % respondents after intervention with p value=0.000. Patient’s medication compliance also increased, from 6 (30 % respondents before intervention to 20 (100% respondents after intervention, with p value= 0.000. Patient compliance on exercise also increase from 2 (10% respondents before intervention, become 19 (95% respondents after intervention, with p value=0.000. Discussion: Self management intervention could improve patient’s knowledge, problem-solving skills, and self-effi cacy. Self management should be done after the patient had understand their disease and realized the importance of self-care. Community health nurses were expected to implement self management as one of nursing intervention, so that patient compliance on their treatment can be increased. Key words: Self management intervention, compliance, patient DM type 2

  6. Design of UAV video encoding system based on DM368%基于 DM368的无人机视频压缩系统的设计

    Institute of Scientific and Technical Information of China (English)

    吴笑天; 鲁剑锋; 王宇庆; 陈典兵

    2014-01-01

    无人机视频数据的实时压缩处理是无人机的关键技术之一.本文以 DM368为核心处理器,以嵌入式 Linux 为板级操作系统,设计出一套可供无人机使用的低功耗视频实时压缩系统.与传统的嵌入式视频 H264软件编码压缩系统相比,本系统依赖 DM368内嵌的视频协处理硬核处理 H264编码,具有功耗低,处理延迟小,外部电路简单等诸多优势.同时系统以嵌入式 Linux 为板级操作系统,实时性强,可稳定高效的管理视频采集、硬核调度、串口通讯、数据输出等诸多任务.试验结果表明,本系统在处理 PAL 制式为视频源的 H264编码任务时,平均功耗仅为1.5 W,具有功耗低、成本低、体积小、重量轻、实时性强等诸多优势,能够满足无人机视频压缩的应用需求.%Real-time UAV video data compression is one of the key technology of UAV.In this paper we propose a real-time,low-power video encoding system design,based on DM368 processor and em-bedded Linux operating system.Compared with traditional embedded video H264 encoding system de-sign,we use DM368 inner video image co-processors to process the encoding task,which has the ad-vantage of low-power consumption,low processing delay,and simple external circuit design.Further-more,we utilize the real-time,stable and efficient embedded Linux operating system,capable of pro-cessing multi-task,such as video capturing,hard-core scheduling,serial communication,and data transmission.Experiment shows that the average power consumption of our system is only 1.5 W when dealing with the PAL video H264 coding task.Our design meet the requirement of UAV video compression task efficiently,with the advantage of low-power,low-cost,small-size,light-weight, and good real-time quality.

  7. DM100 AND DM1200 MELTER TESTING WITH HIGH WASTE LOADING GLASS FORMULATIONS FOR HANFORD HIGH-ALUMINUM HLW STREAMS

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; KOT WK; PEGG IL; JOSEPH I

    2009-12-30

    This Test Plan describes work to support the development and testing of high waste loading glass formulations that achieve high glass melting rates for Hanford high aluminum high level waste (HLW). In particular, the present testing is designed to evaluate the effect of using low activity waste (LAW) waste streams as a source of sodium in place ofchemical additives, sugar or cellulose as a reductant, boehmite as an aluminum source, and further enhancements to waste processing rate while meeting all processing and product quality requirements. The work will include preparation and characterization of crucible melts in support of subsequent DuraMelter 100 (DM 100) tests designed to examine the effects of enhanced glass formulations, glass processing temperature, incorporation of the LAW waste stream as a sodium source, type of organic reductant, and feed solids content on waste processing rate and product quality. Also included is a confirmatory test on the HLW Pilot Melter (DM1200) with a composition selected from those tested on the DM100. This work builds on previous work performed at the Vitreous State Laboratory (VSL) for Department of Energy's (DOE's) Office of River Protection (ORP) to increase waste loading and processing rates for high-iron HLW waste streams as well as previous tests conducted for ORP on the same waste composition. This Test Plan is prepared in response to an ORP-supplied statement of work. It is currently estimated that the number of HLW canisters to be produced in the Hanford Tank Waste Treatment and Immobilization Plant (WTP) is about 12,500. This estimate is based upon the inventory ofthe tank wastes, the anticipated performance of the sludge treatment processes, and current understanding of the capability of the borosilicate glass waste form. The WTP HLW melter design, unlike earlier DOE melter designs, incorporates an active glass bubbler system. The bubblers create active glass pool convection and thereby improve heat

  8. Adiponectin may improve osseointegration of dental implants in T2DM patients.

    Science.gov (United States)

    Bai, Yun; Yin, Guozhu; Luo, En

    2011-08-01

    Type 2 diabetes mellitus (T2DM) is the most common form of diabetes. Compared with the general population, a higher failure rate is seen in T2DM patients. There is also evidence that chronically high levels of plasma glycemia leads to inflammatory effect and a negative influence on bone formation and remodeling, and reduce osseointegration of implants. Recently studies reveal that adiponectin is an insulin-sensitizing adipokine, and closely associated with T2DM. Adiponectin has potent anti-inflammatory properties and has been shown to increase bone density by inhibiting osteoclast formation and promoting the formation of osteoblasts. We therefore hypothesize systemically infused or locally used adiponectin could accelerate osseointegration of dental implants in T2DM. Our hypothesis could help to create an option to improve success ratio of dental implants in T2DM by the replenishment of adiponectin in T2DM patients.

  9. IceCube Events from Heavy DM decays through the Right-handed Neutrino Portal

    CERN Document Server

    Ko, P

    2015-01-01

    The recently observed IceCube PeV events could be due to heavy dark matter (DM) decay. In this paper, we propose a simple DM model with extra $U(1)_X$ gauge symmetry and bridge it with standard model particles through heavy right-handed neutrino. The Dirac fermion DM $\\chi$ with mass ~5 PeV can dominantly decay into a dark Higgs ($\\phi$), the SM Higgs ($h$) and a neutrino ($\

  10. Performance Study of CM/DM Discrimination Network for Conducted EMI Diagnosis

    Institute of Scientific and Technical Information of China (English)

    ZHAOYang; SEEKyeYak

    2003-01-01

    This paper compares the discrimination capability for four different types of Common-mode and Differential-mode Discrimination networks (CM/DM DN). The insertion losses and mode rejection performances for the four CM/DM DNs are measured. Based on the measured results, the CM/DM DN with the best discrimination capability is chosen for the purpose of conducted EMI diagnosis. A practical example is shown to demonstrate the ease of EMI diagnosis, with the aid of the chosen CM/DM DN.

  11. Organizacijska kultura v podjetju dm drogerie markt d.o.o.

    OpenAIRE

    Renko, Jure

    2016-01-01

    Organizacijska kultura pomembno vpliva na uspešnost posameznika v organizaciji in uspeh podjetja pri strankah. V diplomskem delu smo preučili organizacijsko kulturo v podjetju dm drogerie markt d.o.o., ki je del koncerna dm. Management v koncernu dm se zaveda pomembnosti tega kompleksnega in dinamičnega pojava. V dm Slovenija smo naredili raziskavo s pomočjo anketnega vprašalnika, kjer smo izmerili trenutno in želeno organizacijsko kulturo z vidika zaposlenih in s stališča lastnikov. Na podla...

  12. Toward electroweak scale cold dark matter with local dark gauge symmetry and beyond the DM EFT

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Pyungwon, E-mail: pko@kias.re.kr [School of Physics, Korea Institute for Advanced Study 85 Hoegiro, Dongdaemun-gu, Seoul 02455 (Korea, Republic of)

    2016-06-21

    In this talk, I describe a class of electroweak (EW) scale dark matter (DM) models where its stability or longevity are the results of underlying dark gauge symmetries: stable due to unbroken local dark gauge symmetry or topology, or long-lived due to the accidental global symmetry of dark gauge theories. Compared with the usual phenomenological dark matter models (including DM EFT or simplified DM models), DM models with local dark gauge symmetries include dark gauge bosons, dark Higgs bosons and sometimes excited dark matter. And dynamics among these fields are completely fixed by local gauge principle. The idea of singlet portals including the Higgs portal can thermalize these hidden sector dark matter very efficiently, so that these DM could be easily thermal DM. I also discuss the limitation of the usual DM effective field theory or simplified DM models without the full SM gauge symmetry, and emphasize the importance of the full SM gauge symmetry and renormalizability especially for collider searches for DM.

  13. Myotonic dystrophy (DM1) and dysphagia: the need for dysphagia management guidelines and an assessment tool

    NARCIS (Netherlands)

    LaDonna, K.A.; Koopman, W.J.H.; Venance, S.L.

    2011-01-01

    Myotonic dystrophy (DM1) is the most prevalent muscular dystrophy occurring in adulthood. DM1 is a multi-systemic disorder resulting in early-onset cataracts, cardiac rhythm problems, muscle weakness, ptosis, and cognitive and psychiatric manifestations. Dysphagia is one of the most problematic

  14. Comparative experimental and theoretical investigations of the DM neutron moisture probe

    DEFF Research Database (Denmark)

    Ølgaard, Povl Lebeck; Haahr, Vagner

    1967-01-01

    Theoretical and experimental investigations of the Danish produced DM subsurface moisture probe have been carried out at the Research Establishment Risö, and the results obtained are presented in this paper. The DM probe contains an Am-Be fast neutron source and has a glass scintillator containi...

  15. Registration of an oilseed sunflower germplasm HA-DM1 resistant to sunflower downy mildew

    Science.gov (United States)

    HA-DM1 (Reg. No.xxx, PI 674793) sunflower (Helianthus annuus L.) germplasm was developed and released cooperatively by the USDA-ARS, Sunflower and Plant Biology Research Unit and the North Dakota Agricultural Experiment Station in 2015. HA-DM1 is a BC2F4 derived oilseed maintainer line from the cros...

  16. Foundational Forces & Hidden Variables in Technology Commercialization

    Science.gov (United States)

    Barnett, Brandon

    2011-03-01

    The science of physics seems vastly different from the process of technology commercialization. Physics strives to understand our world through the experimental deduction of immutable laws and dependent variables and the resulting macro-scale phenomenon. In comparison, the~goal of business is to make a profit by addressing the needs, preferences, and whims of individuals in a market. It may seem that this environment is too dynamic to identify all the hidden variables and deduct the foundational forces that impact a business's ability to commercialize innovative technologies. One example of a business ``force'' is found in the semiconductor industry. In 1965, Intel co-founder Gordon Moore predicted that the number of transistors incorporated in a chip will approximately double every 24 months. Known as Moore's Law, this prediction has become the guiding principle for the semiconductor industry for the last 40 years. Of course, Moore's Law is not really a law of nature; rather it is the result of efforts by Intel and the entire semiconductor industry. A closer examination suggests that there are foundational principles of business that underlie the macro-scale phenomenon of Moore's Law. Principles of profitability, incentive, and strategic alignment have resulted in a coordinated influx of resources that has driven technologies to market, increasing the profitability of the semiconductor industry and optimizing the fitness of its participants. New innovations in technology are subject to these same principles. So, in addition to traditional market forces, these often unrecognized forces and variables create challenges for new technology commercialization. In this talk, I will draw from ethnographic research, complex adaptive theory, and industry data to suggest a framework with which to think about new technology commercialization. Intel's bio-silicon initiative provides a case study.

  17. Interference effects of two scalar boson propagators on the LHC search for the singlet fermion DM

    CERN Document Server

    Ko, P

    2016-01-01

    A gauge invariant UV-completion for singlet fermion DM interacting with the standard model (SM) particles involves a new singlet scalar. Therefore the model contains two scalar mediators, mixtures of the SM Higgs boson and a singlet scalar boson. Collider phenomenology of the interference effect between these two scalar propagators is studied in this work. This interference effect can be either constructive or destructive in the DM production cross section depending on both singlet scalar and DM masses, and it will soften the final state jets in the full mass region. Applying the CMS mono-jet search to our model, we find the interference effect plays a very important role in the DM search sensitivity, and the DM production cross section of our model is more than one order of magnitude below the LHC sensitivity at current stage.

  18. Interference effects of two scalar boson propagators on the LHC search for the singlet fermion DM

    Science.gov (United States)

    Ko, P.; Li, Jinmian

    2017-02-01

    A gauge invariant UV-completion for singlet fermion DM interacting with the standard model (SM) particles involves a new singlet scalar. Therefore the model contains two scalar mediators, mixtures of the SM Higgs boson and a singlet scalar boson. Collider phenomenology of the interference effect between these two scalar propagators is studied in this work. This interference effect can be either constructive or destructive in the DM production cross section depending on both singlet scalar and DM masses, and it will soften the final state jets in the full mass region. Applying the CMS mono-jet search to our model, we find the interference effect plays a very important role in the DM search sensitivity, and the DM production cross section of our model is more than one order of magnitude below the LHC sensitivity at current stage.

  19. 酸性皂洗剂DM-1530应用实践%The application practice of the acid soaping agent DM-1530

    Institute of Scientific and Technical Information of China (English)

    季金霞; 胡灿辉; 吴少新

    2009-01-01

    酸性皂洗剂DM-1530将酸中和与皂洗合二为一,可以替代醋酸起到中和作用,而且皂洗效果极佳.以大红色纯棉双面针织布为例,分析了不同pH值、DM-1530用量、水的硬度对DM-1530皂洗效果的影响,并通过生产实践,介绍了传统工艺和DM-1530工艺的流程,比较分析了二者的pH值变化情况、皂洗牢度以及生产成本.表明,使用DM-1530可省去皂洗前的酸中和工艺,节约能源,减少污水排放,缩短工艺流程,提高工作效率,皂洗效果良好.

  20. Nonlinear Phenomena in Complex Systems: From Nano to Macro Scale

    CERN Document Server

    Stanley, H

    2014-01-01

    Topics of complex system physics and their interdisciplinary applications to different problems in seismology, biology, economy, sociology,  energy and nanotechnology are covered in this new work from renowned experts in their fields.  In  particular, contributed papers contain original results on network science, earthquake dynamics, econophysics, sociophysics, nanoscience and biological physics. Most of the papers use interdisciplinary approaches based on statistical physics, quantum physics and other topics of complex system physics.  Papers on econophysics and sociophysics are focussed on societal aspects of physics such as, opinion dynamics, public debates and financial and economic stability. This work will be of interest to statistical physicists, economists, biologists, seismologists and all scientists working in interdisciplinary topics of complexity.

  1. STUDY OF PREVALENCE OF THYROID DYSFUNCTION IN TYPE 2 DM IN RURAL POPULATION

    Directory of Open Access Journals (Sweden)

    Nanjil Kumaran

    2015-08-01

    Full Text Available Thyroid disease and diabetes are the two common endocrine disorders encountered in the clinical practice. Type 2 DM is a growing problem in our country and we have observed that many patients are associated with thyroid dysfunction later in their life. The aim of the study is to investigate the prevalence of thyroid dysfunction in patients with Type 2 DM in rural population. METHODS AND MATERIALS : A total of 100 patients attending diabetic clinic in Rajah Muthiah Medical College and Hospital were enrolled in the study and were randomized into test and control groups.70 Type 2 DM patients aged more than 18 years of both genders, Type II DM was diagnosed as pati ents who initially respond to OHA were included in the test group. Patient with thyroid dysfunction, Type 1 DM, chronic kidney disease were excluded. 30 Patients age matched control group without any history of diabetes and thyroid disease was taken for the study. RESULTS : In this study abnormal thyroid function was seen in 17 patients of Type 2 DM and remaining had normal thyroid function. The study shows prevalence of thyroid dysfunction is more in males compared with females. 10 out of 29 (34.4% males had thyroid dysfunction compared with that 7 out of 41 (17% females are suffering from thyroid dysfunction. CONCLUSION : There is growing evidence of an association between thyroid dysfunction and diabetes. Our study shows that higher prevalence of abnormal thyroid hormone levels in Type 2 DM.

  2. Investigation of the interaction of deltamethrin (DM) with human serum albumin by multi-spectroscopic method

    Science.gov (United States)

    Wang, Jiaman; Ma, Liang; Zhang, Yuhao; Jiang, Tao

    2017-02-01

    The interaction of Deltamethrin (DM) with human serum albumin (HSA) under the condition of simulating human blood pH environment (pH = 7.4) was investigated by fluorescence, UV-Vis absorbance and circular dichroism (CD) spectroscopy. It was shown that DM was a static quencher of HSA. The binding constants (Ka) are 3.598 × 104 L mol-1 (25 °C); the thermodynamic parameters (ΔH = -3.269 × 104 kJ mol-1, ΔS = -22.81 kJ mol-1 k-1, ΔG = -25889.8 kJ mol-1) obtained with the thermodynamic equation. The hydrogen bond and Vander Waals were the main driving force. The effect of DM on the conformation of HSA was observed by three-dimensional (3D) fluorescence and circular dichroism spectra, indicating that the interaction between DM and HSA was achieved through the binding of DM with the tryptophan and tyrosine residues of HSA. The study on the interaction of DM and Bovine Serum Albumin (BSA) was researched and compared. Difference exists in the interactions of with each of the serum albumins. We will verify and supplement that DM residue in animals and human metabolism, toxicology and other mechanisms are different.

  3. Reduced expression of the normal DMPK allele in a congenital DM patient

    Energy Technology Data Exchange (ETDEWEB)

    Funanage, V.L.; Carango, P.; Moses, R.M.; Marks, H.G. [Alfred I. duPont Institute, Wilmington, DE (United States)

    1994-09-01

    Both adult-onset and congenital myotonic dystrophy (DM) are autosomal dominant disorders caused by triplet repeat expansions within the 3{prime} untranslated region of the DM protein kinase (DMPK) gene. The size of the repeat region shows a positive correlation with disease severity; in general, the triplet repeat expansions in congenital DM patients are larger than those found in adult DM individuals. In an adult DM patient, the expanded allele of 133 repeats reduced both the synthesis and processing of DMPK mRNA, whereas expression from the unexpanded allele remained unaffected. However, in both muscle and skin tissues from a congenital DM individual, DMPK mRNA expression from the unexpanded allele was also reduced. This reduced expression was maintained in fibroblasts cultured from a skin biospy of the patient; however, normal expression of the unexpanded allele occurred in cultured myoblasts established from this patient`s muscle biopsy. To determine if the expanded repeat exerts a trans effect on DMPK gene expression, we have separated the normal and mutant DMPK alleles from the cogenital DM skin fibroblasts by somatic cell hybridization. Hybrid clones containing only the normal DMPK gene still produced reduced levels of DMPK mRNA, indicating that the reduced expression from the normal allele is due to a cis effect. Cultured skin fibroblasts from the congenital DM patient were exposed to 5-azacytidine to determine if demethylation of the DMPK gene could restore proper expression of the normal allele. We are currently analyzing DMPK mRNA levels in these cells and determining if a difference in the methylation patterns of the normal DMPK alleles from adult and congenital DM patients accounts for this effect.

  4. [Study of a DNA sequence from brine shrimp artemia containing a novel DM domain].

    Science.gov (United States)

    Zeng, Hui; Song, Wen Qin; Chen, Rui Yang

    2004-10-01

    Sex-determining mechanisms are highly variable between phyla. However, there is an apparent exception in which structurally and functionally related genes control sex determination in different phyla: the sexual regulators DSX of Drosophila melanogaster and MAB-3 of Caenorhabditis elegans both containing a DNA-binding motif, DM domain. Proteins containing the domain may also play a role in sexual development of vertebrates. For examples, both the human DMRT1 (doublesex and mab-3 related transcription factor 1) gene and mouse Dmrt1 gene are necessary for male development. In this paper, through the degenerated PCR, a DNA fragment ADM was amplified out from genomic DNA of brine shrimp, Artemia sinica from YunCheng Salt Lake, Shanxi, China and Artemia parthenogenetica from GaHai, Qinghai, China, respectively. ADM encodes 47 amino acids and is highly homologous to amino acid sequence of the known DM domains. By comparing total of 27 DM domains in distant related species, a phylogenic tree of DM domain was constructed. In the tree, these DM domains were divided into different branches according to their subtypes. Among the DM domains that were compared, ADM is most homologous to the DM domain contained in human DMRT3 and mouse Dmrt3, which shares 83% identity between them. In addition, the same length of ADM could also be amplified out from cDNA of Artemia sinica and Artemia parthenogenetica, which indicated that ADM was expressed and located in one exon. The DM domain in brine shrimp reported here would make it possible for cloning the full-length cDNA containing the DM domain and further elucidating their functions.

  5. Dm5-HT2B: Pharmacological Characterization of the Fifth Serotonin Receptor Subtype of Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Wolfgang Blenau

    2017-05-01

    Full Text Available Serotonin (5-hydroxytryptamine, 5-HT is an important regulator of physiological and behavioral processes in both protostomes (e.g., insects and deuterostomes (e.g., mammals. In insects, serotonin has been found to modulate the heart rate and to control secretory processes, development, circadian rhythms, aggressive behavior, as well as to contribute to learning and memory. Serotonin exerts its activity by binding to and activating specific membrane receptors. The clear majority of these receptors belong to the superfamily of G-protein-coupled receptors. In Drosophila melanogaster, a total of five genes have been identified coding for 5-HT receptors. From this family of proteins, four have been pharmacologically examined in greater detail, so far. While Dm5-HT1A, Dm5-HT1B, and Dm5-HT7 couple to cAMP signaling cascades, the Dm5-HT2A receptor leads to Ca2+ signaling in an inositol-1,4,5-trisphosphate-dependent manner. Based on sequence similarity to homologous genes in other insects, a fifth D. melanogaster gene was uncovered coding for a Dm5-HT2B receptor. Knowledge about this receptor’s pharmacological properties is very limited. This is quite surprising because Dm5-HT2B has been attributed to distinct physiological functions based on genetic interference with its gene expression. Mutations were described reducing the response of the larval heart to 5-HT, and specific knockdown of Dm5-HT2B mRNA in hemocytes resulted in a higher susceptibility of the flies to bacterial infection. To gain deeper understanding of Dm5-HT2B’s pharmacology, we evaluated the receptor’s response to a series of established 5-HT receptor agonists and antagonists in a functional cell-based assay. Metoclopramide and mianserin were identified as two potent antagonists that may allow pharmacological interference with Dm5-HT2B signaling in vitro and in vivo.

  6. Myotonic dystrophy type 1 (DM1): a triplet repeat expansion disorder.

    Science.gov (United States)

    Kumar, Ashok; Agarwal, Sarita; Agarwal, Divya; Phadke, Shubha R

    2013-06-15

    Myotonic dystrophy is a progressive multisystem genetic disorder affecting about 1 in 8000 people worldwide. The unstable repeat expansions of (CTG)n or (CCTG)n in the DMPK and ZNF9 genes cause the two known subtypes of myotonic dystrophy: (i) myotonic dystrophy type 1 (DM1) and (ii) myotonic dystrophy type 2 (DM2) respectively. There is currently no cure but supportive management helps equally to reduce the morbidity and mortality and patients need close follow up to pay attention to their clinical problems. This review will focus on the clinical features, molecular view and genetics, diagnosis and management of DM1.

  7. Qualidade de vida e sintomas depressivos em adolescentes com Diabetes Mellitus tipo 1 (DM1)

    OpenAIRE

    Gabriela de Oliveira Pintar

    2015-01-01

    O objetivo geral deste trabalho foi avaliar a presença de sintomas depressivos e as possíveis influências do Diabetes Mellitus tipo I (DM1) na qualidade de vida de adolescentes com esta doença. Para alcançar tal objetivo foram avaliados adolescentes com DM1 (58) e sem DM1 (61) completando um total de 119 adolescentes avaliados; de ambos os sexos. Os locais de coleta de dados foram o Ambulatório de Endocrinologia da Criança e do Adolescente (ECA) do Hospital das Clínicas da Faculdade de Medici...

  8. Studies on the s_dm.t=f verb form in Classical Egyptian

    OpenAIRE

    Zonhoven, Ludovicus Martinus Johannes

    1997-01-01

    This study is devoted to some synchronic aspects of the sDm.t=f verb form, primarily its meaning and uses in Classical Egyptian. In the introduction some attention is paid to the history of the studies of the form and its origin, an aspect which will receive no further consideration. In accordance with present common opinion the sDm.t=f is here considered to belong to the suffix conjugation. Ch. I is primarily concerned with the active Dr sDm.t=f construction, but begins with a general introd...

  9. 基于DM365的便携监控系统解决方案%PortabIe Monitoring System SoIution Based on DM365

    Institute of Scientific and Technical Information of China (English)

    桑赫; 林岩; 张烽

    2015-01-01

    本文提出了一种基于TMS320 DM365芯片的便携监控系统解决方案。设计以 TMS320 DM365为核心硬件设备,在满足基本功能的基础上,尽量减小体积、降低功耗。编写了基于嵌入式 Linux系统的应用程序,完成了音频/视频采集、存储等功能,并设计了有效的电源管理方案,实现了便携监控系统与 Android手机间的数据交互,提升了该系统的易用性。%A portable monitoring system based on TMS320DM365 chip is proposed.The design takes TMS320DM365 as the core of the hardware devices,it minimizes the size and the power consumption as possible while satisfying the basic functions.The collection and storage of audio/video,effective power management scheme and data exchange between portable monitoring device and Android mobile phone are realized through writing application programs based on the embedded Linux system,so the usability of the system is enhanced.

  10. Educational Interactive eBook for Newly Diagnosed Children with T1DM: Children’s Role in Design

    Directory of Open Access Journals (Sweden)

    Damyanka Tsvyatkova

    2015-12-01

    Full Text Available Some of the central concerns in technology development for healthcare interventions in Type 1 Diabetes Mellitus (T1DM are how to involve young patients and their families in the design process and what methods, including artbased activities, would best facilitate a child expressing their innermost feelings when applying child-centered and participatory approaches. This paper describes a variety of artbased tools and methods (e.g. Cooperative Inquiry (CI and Informant Design (ID used in the creative design processes for defining features and eliciting the content of the stories, plots, roles, characters, images, animations, languages, etc., that will inform the design of an educational interactive eBook for newly diagnosed children (aged 8-12 years with T1DM. The articulation of design ideas through modeling plasticine figures, drawing and thinking aloud were generated and collected in three workshop sessions organized for diabetic and healthy children who had different roles in and contributions to the design process. Successfully collected data will be used to build a series of low fidelity paper based eBook prototypes.

  11. Sense and Antisense DMPK RNA Foci Accumulate in DM1 Tissues during Development.

    Directory of Open Access Journals (Sweden)

    Lise Michel

    Full Text Available Myotonic dystrophy type 1 (DM1 is caused by an unstable expanded CTG repeat located within the DMPK gene 3'UTR. The nature, severity and age at onset of DM1 symptoms are very variable in patients. Different forms of the disease are described, among which the congenital form (CDM is the most severe. Molecular mechanisms of DM1 are well characterized for the adult form and involve accumulation of mutant DMPK RNA forming foci in the nucleus. These RNA foci sequester proteins from the MBNL family and deregulate CELF proteins. These proteins are involved in many cellular mechanisms such as alternative splicing, transcriptional, translational and post-translational regulation miRNA regulation as well as mRNA polyadenylation and localization. All these mechanisms can be impaired in DM1 because of the deregulation of CELF and MBNL functions. The mechanisms involved in CDM are not clearly described. In order to get insight into the mechanisms underlying CDM, we investigated if expanded RNA nuclear foci, one of the molecular hallmarks of DM1, could be detected in human DM1 fetal tissues, as well as in embryonic and neonatal tissues from transgenic mice carrying the human DMPK gene with an expanded CTG repeat. We observed very abundant RNA foci formed by sense DMPK RNA and, to a lesser extent, antisense DMPK RNA foci. Sense DMPK RNA foci clearly co-localized with MBNL1 and MBNL2 proteins. In addition, we studied DMPK sense and antisense expression during development in the transgenic mice. We found that DMPK sense and antisense transcripts are expressed from embryonic and fetal stages in heart, muscle and brain and are regulated during development. These results suggest that mechanisms underlying DM1 and CDM involved common players including toxic expanded RNA forming numerous nuclear foci at early stages during development.

  12. Mitochondrial DNA backgrounds might modulate diabetes complications rather than T2DM as a whole.

    Directory of Open Access Journals (Sweden)

    Alessandro Achilli

    Full Text Available Mitochondrial dysfunction has been implicated in rare and common forms of type 2 diabetes (T2DM. Additionally, rare mitochondrial DNA (mtDNA mutations have been shown to be causal for T2DM pathogenesis. So far, many studies have investigated the possibility that mtDNA variation might affect the risk of T2DM, however, when found, haplogroup association has been rarely replicated, even in related populations, possibly due to an inadequate level of haplogroup resolution. Effects of mtDNA variation on diabetes complications have also been proposed. However, additional studies evaluating the mitochondrial role on both T2DM and related complications are badly needed. To test the hypothesis of a mitochondrial genome effect on diabetes and its complications, we genotyped the mtDNAs of 466 T2DM patients and 438 controls from a regional population of central Italy (Marche. Based on the most updated mtDNA phylogeny, all 904 samples were classified into 57 different mitochondrial sub-haplogroups, thus reaching an unprecedented level of resolution. We then evaluated whether the susceptibility of developing T2DM or its complications differed among the identified haplogroups, considering also the potential effects of phenotypical and clinical variables. MtDNA backgrounds, even when based on a refined haplogroup classification, do not appear to play a role in developing T2DM despite a possible protective effect for the common European haplogroup H1, which harbors the G3010A transition in the MTRNR2 gene. In contrast, our data indicate that different mitochondrial haplogroups are significantly associated with an increased risk of specific diabetes complications: H (the most frequent European haplogroup with retinopathy, H3 with neuropathy, U3 with nephropathy, and V with renal failure.

  13. Nature of Technology: Implications for design, development, and enactment of technological tools in school science classrooms

    Science.gov (United States)

    Waight, Noemi; Abd-El-Khalick, Fouad

    2012-12-01

    This position paper provides a theory-based explanation informed by philosophy of technology (PoT) of the recurrent documented patterns often associated with attempts to enact technology-supported, inquiry-based approaches in precollege science classrooms. Understandings derived from the history of technological development in other domains (e.g. medicine, transportation, and warfare) reveal numerous parallels that help to explain these recurrent patterns. Historical analyses of major technologies reveal a conglomerate of factors that interact to produce benefits, as well as intended and unintended consequences. On a macro-scale, PoT facilitates understandings of how technologies interact and are impacted by individuals, society, institutions, economy, politics, and culture. At the micro-level, and most relevant to science education, PoT engages the inherent nature of technology along a number of key dimensions: role of culture and values, notions of technological progression, technology as part of systems, technological diffusion, technology as a fix, and the notions of expertise. Overall, the present analysis has implications for the design, development, implementation, and adoption of technological tools for use in precollege science education, and highlights the role of technology as both artifact and process.

  14. Microstructure evolution and kinetic analysis of DM hot-work die steels during tempering

    Energy Technology Data Exchange (ETDEWEB)

    Zhou Qingchun, E-mail: qingchun@shu.edu.cn [School of Materials Science and Engineering, Shanghai University, Shanghai 200072 (China); Wu Xiaochun; Shi Nannan; Li Junwan; Min Na [School of Materials Science and Engineering, Shanghai University, Shanghai 200072 (China)

    2011-07-15

    Highlights: {yields} Hardness of the DM steels decreases continuously with increasing temperature. {yields} Increasing temperatures results in intra-lath carbides precipitate and coarsen significantly. {yields} The values of n indicate a growth mechanism in coarsening of large spheroids on dislocations. {yields} Mn in DM steels dissolves into carbides and retards the coarsening of carbides at high temperature. - Abstract: Utilizing the hardness measurement, an attempt is made to study the tempering kinetics of DM hot-work die steels, combined with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results demonstrate that the increasing of tempering temperature will result in precipitation and coarsening of the intra-lath spherical carbides. Meanwhile, it is significantly observed the dissolution of needle-like Mo2C carbides with increasing of tempering temperatures. The growth mechanism of carbides is coarsening of large spheroids after complete and impingement precipitation on dislocations. In addition, the activation energy {Delta}H of DM steels during additional tempering between 620 deg. C and 700 deg. C is slightly higher than that of the diffusion of usual alloying elements (e.g. Cr, V, Mo and W) in ferrite, which may be attributed to a higher Mn content in DM steels that dissolves into carbides and retardsthe coarsening of carbides at high temperature.

  15. Complementarity of DM searches in a consistent simplified model: the case of Z{sup ′}

    Energy Technology Data Exchange (ETDEWEB)

    Jacques, Thomas [SISSA and INFN,via Bonomea 265, 34136 Trieste (Italy); Katz, Andrey [Theory Division, CERN,CH-1211 Geneva 23 (Switzerland); Département de Physique Théorique and Center for Astroparticle Physics (CAP),Université de Genève, 24 quai Ansermet, CH-1211 Genève 4 (Switzerland); Morgante, Enrico; Racco, Davide [Département de Physique Théorique and Center for Astroparticle Physics (CAP),Université de Genève, 24 quai Ansermet, CH-1211 Genève 4 (Switzerland); Rameez, Mohamed [Département de Physique Nucléaire et Corpusculaire,Université de Genève, 24 quai Ansermet, CH-1211 Genève 4 (Switzerland); Riotto, Antonio [Département de Physique Théorique and Center for Astroparticle Physics (CAP),Université de Genève, 24 quai Ansermet, CH-1211 Genève 4 (Switzerland)

    2016-10-14

    We analyze the constraints from direct and indirect detection on fermionic Majorana Dark Matter (DM). Because the interaction with the Standard Model (SM) particles is spin-dependent, a priori the constraints that one gets from neutrino telescopes, the LHC, direct and indirect detection experiments are comparable. We study the complementarity of these searches in a particular example, in which a heavy Z{sup ′} mediates the interactions between the SM and the DM. We find that for heavy dark matter indirect detection provides the strongest bounds on this scenario, while IceCube bounds are typically stronger than those from direct detection. The LHC constraints are dominant for smaller dark matter masses. These light masses are less motivated by thermal relic abundance considerations. We show that the dominant annihilation channels of the light DM in the Sun and the Galactic Center are either bb̄ or tt̄, while the heavy DM annihilation is completely dominated by Zh channel. The latter produces a hard neutrino spectrum which has not been previously analyzed. We study the neutrino spectrum yielded by DM and recast IceCube constraints to allow proper comparison with constraints from direct and indirect detection experiments and LHC exclusions.

  16. Complementarity of DM Searches in a Consistent Simplified Model: the Case of Z'

    CERN Document Server

    Jacques, Thomas; Morgante, Enrico; Racco, Davide; Rameez, Mohamed; Riotto, Antonio

    2016-01-01

    We analyze the constraints from direct and indirect detection on fermionic Majorana Dark Matter (DM). Because the interaction with the Standard Model (SM) particles is spin-dependent, a priori the constraints that one gets from neutrino telescopes, the LHC and direct detection experiments are comparable. We study the complementarity of these searches in a particular example, in which a heavy $Z'$ mediates the interactions between the SM and the DM. We find that in most cases IceCube provides the strongest bounds on this scenario, while the LHC constraints are only meaningful for smaller dark matter masses. These light masses are less motivated by thermal relic abundance considerations. We show that the dominant annihilation channels of the light DM in the Sun are either $b \\bar b$ or $t \\bar t$, while the heavy DM annihilation is completely dominated by $Zh$ channel. The latter produces a hard neutrino spectrum which has not been previously analyzed. We study the neutrino spectrum yielded by DM and recast Ice...

  17. Routine daily physical activity and glucose variations are strongly coupled in adults with T1DM.

    Science.gov (United States)

    Farabi, Sarah S; Carley, David W; Cinar, Ali; Quinn, Lauretta

    2015-12-01

    Type 1 Diabetes (T1DM) is characterized by altered glucose homeostasis resulting in wide glucose variations throughout a 24-h period. The relationship between routine daily physical activity and glucose variations has not been systematically investigated in adults with T1DM. The objectives of this study were to characterize and quantify the relationship between routine daily activity and glucose variations in a small group of adults with T1DM. Adults with T1DM treated with an insulin pump were recruited for the study. Over a 3-day period, glucose variations were monitored with a continuous glucose monitoring system (CGMS) and routine daily physical activity was assessed using an accelerometer-based physical activity-monitoring band. Simultaneous glucose and physical activity data for one 24-h period were used for analysis. Cross-correlation function and wavelet coherence analyses were employed to quantify the coupling between physical activity and glucose. Twelve subjects were included in the analysis. Cross-correlation function analysis revealed strong coupling between activity and glucose. Wavelet Coherence demonstrated that slower oscillations (120-340 min) of glucose and physical activity exhibited significantly greater coherence (F = 12.6, P < 0.0001) than faster oscillations (10 and 120 min). Physical activity and glucose demonstrate strong time and frequency-dependent coupling throughout a 24-h time period in adults with T1DM.

  18. Replacing single-view mediolateral oblique (MLO) digital mammography (DM) with synthesized mammography (SM) with digital breast tomosynthesis (DBT) images: Comparison of the diagnostic performance and radiation dose with two-view DM with or without MLO-DBT.

    Science.gov (United States)

    Kang, Hyo-Jin; Chang, Jung Min; Lee, Joongyub; Song, Sung Eun; Shin, Sung Ui; Kim, Won Hwa; Bae, Min Sun; Moon, Woo Kyung

    2016-11-01

    To evaluate the diagnostic performance and radiation dose of single view cranio-caudal (CC) digital mammography (DM) plus mediolateral oblique (MLO) digital breast tomosynthesis (DBT) combined with synthesized mammography (SM) in comparison with two-view DM with or without DBT. This study was approved by our institutional review board, and informed consent was obtained from 130 women. Paired two-view DM and single MLO-DBT with SM images were acquired, and four independent retrospective reading sessions of different combinations of DM, SM and DBT were performed for the presence of malignant tumors using jackknife alternative free-response receiver operator curve (JAFROC) methods. The diagnostic performances and average glandular dose (AGD) were compared between different combinations of DM, SM and DBT. Of 159 lesions in 130 patients, 27 were malignant. When using MLO-DBT with SM instead of MLO-DM, a significantly higher sensitivity (P=0.016) and specificity (P=0.012) were noted than with two-view DM, and comparable figure of merit (FOM), sensitivity, and specificity to two-view DM with DBT were noted. The mean AGD of CC-DM plus MLO-DBT with SM was 5.78mGy±1.06 per patient, which was significantly lower than that with two-view DM with MLO-DBT (8.45mGy±1.32; P <0.001) and slightly higher than that with two-view DM (5.30mGy±0.63). The combined use of CC-DM plus MLO-DBT with SM showed higher sensitivity and specificity to two-view DM with a smaller AGD increment and comparable diagnostic performance to that of two-view DM with MLO-DBT with a significantly lower mean AGD. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  19. A Peptide/MHCII conformer generated in the presence of exchange peptide is substrate for HLA-DM editing

    OpenAIRE

    Andrea Ferrante; Jack Gorski

    2012-01-01

    The mechanism of HLA-DM (DM) activity is still unclear. We have shown that DM-mediated peptide release from HLA-DR (DR) is dependent on the presence of exchange peptide. However, DM also promotes a small amount of peptide release in the absence of exchange peptide. Here we show that SDS-PAGE separates purified peptide/DR1 complexes (pDR1) into two conformers whose ratio is peptide K d-dependent. In the absence of exchange peptide, DM only releases peptide from the slower migrating conformer. ...

  20. Dark matter, dark radiation and Higgs phenomenology in the hidden sector DM models

    CERN Document Server

    Ko, P

    2015-01-01

    I present a class of hidden sector dark matter (DM) models with local dark gauge symmetries, where DM is stable due to unbroken local dark gauge symmetry, or due topology, or it is long-lived because of some accidental symme- tries, and the particle contents and their dynamics are completely fixed by local gauge symmetries. In these models, one have two types of natural force mediators, dark gauge bosons and dark Higgs boson, which would affect DM and Higgs phenomenology in important ways. I discuss various phenomenological issues including the GeV scale gamma-ray excess from the galactic center (GC), (in)direct detection signatures, dark radiation, Higgs phenomenology and Higgs inflation assisted by dark Higgs.

  1. Separation and Purification of Total Phloroglucinols inDryopteris crassirhizoma with DM-130 Macroporous Adsorption Resin

    Institute of Scientific and Technical Information of China (English)

    Jin Zhe; Huang Jian-ping; Wang He-meng; Ju han-xun; Ren Si-rui; Chang Ying

    2015-01-01

    To improve the purity of the total phloroglucinols fromDryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols fromDryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg• mL-1 and 7 BV (210 mL), respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL• min-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.

  2. Plant insecticide L-canavanine repels Drosophila via the insect orphan GPCR DmX.

    Directory of Open Access Journals (Sweden)

    Christian Mitri

    2009-06-01

    Full Text Available For all animals, the taste sense is crucial to detect and avoid ingesting toxic molecules. Many toxins are synthesized by plants as a defense mechanism against insect predation. One example of such a natural toxic molecule is L-canavanine, a nonprotein amino acid found in the seeds of many legumes. Whether and how insects are informed that some plants contain L-canavanine remains to be elucidated. In insects, the taste sense relies on gustatory receptors forming the gustatory receptor (Gr family. Gr proteins display highly divergent sequences, suggesting that they could cover the entire range of tastants. However, one cannot exclude the possibility of evolutionarily independent taste receptors. Here, we show that L-canavanine is not only toxic, but is also a repellent for Drosophila. Using a pharmacogenetic approach, we find that flies sense food containing this poison by the DmX receptor. DmXR is an insect orphan G-protein-coupled receptor that has partially diverged in its ligand binding pocket from the metabotropic glutamate receptor family. Blockade of DmXR function with an antagonist lowers the repulsive effect of L-canavanine. In addition, disruption of the DmXR encoding gene, called mangetout (mtt, suppresses the L-canavanine repellent effect. To avoid the ingestion of L-canavanine, DmXR expression is required in bitter-sensitive gustatory receptor neurons, where it triggers the premature retraction of the proboscis, thus leading to the end of food searching. These findings show that the DmX receptor, which does not belong to the Gr family, fulfills a gustatory function necessary to avoid eating a natural toxin.

  3. DM235 (sunifiram): a novel nootropic with potential as a cognitive enhancer.

    Science.gov (United States)

    Ghelardini, C; Galeotti, N; Gualtieri, F; Romanelli, M N; Bucherelli, C; Baldi, E; Bartolini, A

    2002-06-01

    DM235 (sunifiram), a new compound structurally related to piracetam, prevented the amnesia induced by scopolamine (1.5 mg kg(-1) i.p.), after intraperitoneal (0.001-0.1 mg kg(-1)) or oral (0.01-0.1 mg kg(-1)) administration, as shown by a passive avoidance test in mice. The antiamnesic effect of DM235 was comparable to that of well-known nootropic drugs such as piracetam (30-100 mg kg(-1) i.p.), aniracetam (100 mg kg(-1) p.o.) or rolipram (30 mg kg(-1) p.o.). DM235 also prevented mecamylamine (20 mg kg(-1) i.p.)-, baclofen (2 mg kg(-1) i.p.)- and clonidine (0.125 mg kg(-1) i.p.)-induced amnesia in the same test. In the Morris water maze test with rats, scopolamine (0.8 mg kg(-1) i.p.) inhibited the reduction of escape latency in both acquisition and retention/retraining tests. DM235 (0.1 mg kg(-1) i.p.), 20 min before each daily acquisition training, prevented the scopolamine-induced memory impairment. DM235 (1 mg kg(-1) i.p.) also reduced the duration of pentobarbitone-induced hypnosis in mice without modifying the induction time of hypnosis. At the highest effective doses, the investigated compound neither impaired motor coordination (rota-rod test), nor modified spontaneous motility and inspection activity (Animex and hole board tests). These results indicate that DM235, a compound structurally related to piracetam, is a novel nootropic endowed with the capability to prevent cognitive deficits at very low doses. Indeed, its potency is about 1,000 times higher than that of the most active piracetam-like compounds.

  4. Polyuria with the Concurrent manifestation of Central Diabetes Insipidus (CDI) & Type 2 Diabetes Mellitus (DM)

    OpenAIRE

    Shin, Hyun-Jong; Kim, Jae-ha; Yi, Joo-Hark; Han, Sang-Woong; Kim, Ho-Jung

    2012-01-01

    We report a rare case of the concurrent manifestation of central diabetes insipidus (CDI) and type 2 diabetes mellitus (DM). A 56 year-old man was diagnosed as a type 2 DM on the basis of hyperglycemia with polyuria and polydipsia at a local clinic two months ago and started an oral hypoglycemic medication, but resulted in no symptomatic improvement at all. Upon admission to the university hospital, the patient's initial fasting blood sugar level was 140 mg/dL, and he showed polydipsic and po...

  5. Technology.

    Science.gov (United States)

    Online-Offline, 1998

    1998-01-01

    Focuses on technology, on advances in such areas as aeronautics, electronics, physics, the space sciences, as well as computers and the attendant progress in medicine, robotics, and artificial intelligence. Describes educational resources for elementary and middle school students, including Web sites, CD-ROMs and software, videotapes, books,…

  6. The combined effect of the T2DM susceptibility genes is an important risk factor for T2DM in non-obese Japanese: a population based case-control study

    Directory of Open Access Journals (Sweden)

    Yamakawa-Kobayashi Kimiko

    2012-02-01

    Full Text Available Abstract Background Type 2 diabetes mellitus (T2DM is a complex endocrine and metabolic disorder. Recently, several genome-wide association studies (GWAS have identified many novel susceptibility loci for T2DM, and indicated that there are common genetic causes contributing to the susceptibility to T2DM in multiple populations worldwide. In addition, clinical and epidemiological studies have indicated that obesity is a major risk factor for T2DM. However, the prevalence of obesity varies among the various ethnic groups. We aimed to determine the combined effects of these susceptibility loci and obesity/overweight for development of T2DM in the Japanese. Methods Single nucleotide polymorphisms (SNPs in or near 17 susceptibility loci for T2DM, identified through GWAS in Caucasian and Asian populations, were genotyped in 333 cases with T2DM and 417 control subjects. Results We confirmed that the cumulative number of risk alleles based on 17 susceptibility loci for T2DM was an important risk factor in the development of T2DM in Japanese population (P P P = 0.88 for trend. Conclusions Our findings indicate that there is an etiological heterogeneity of T2DM between obese/overweight and non-obese subjects.

  7. 基于DM365的人脸检测系统的设计与实现%Design and implementation of human facedetection based on DM365

    Institute of Scientific and Technical Information of China (English)

    张健; 蒋挺

    2012-01-01

    随着安防、人机交互等需求的提出,人脸检测已经成为模式识别领域的一个研究热点,日益受到人们的重视。近年来,随着嵌入式系统的快速发展,出现了基于嵌入式设备的人脸检测系统,由于其具有体积小、成本低、布设方便的优点,逐渐得到了人们的青睐,所以取得了很大的发展。本文主要研究和开发了基于德州仪器DM365视频监控设备的人脸检测系统,针对 Adaboost 的人脸检测算法进行了研究,利用改进的带加权判决函数的级联分类器来提高检测准确率,并给出了在DM365嵌入式视频监控设备上的实现。%The face detection algorithm, currently, receives an increasing attention, and has become a hot topic in the field of the pattern recognition with the requirement of the security guards against theft and the human-computer interaction. With the rapid development of the embedded system, the face detection system based on embedded platform emerged in recent years. And this system has earned the favor of users and developed rapidly due to its advantages of tiny volume, low cost and convenient layout. In this paper, we researched and developed a face detection system based on DM365. The AdaBoost algorithm was improved by employing a cascade classifier with weighted decision function. This improved algorithm enhanced accuracy rate of the detection and was implemented on DM365.

  8. Gene structure and amino acid sequence of Latimeria chalumnae (coelacanth) myelin DM20: phylogenetic relation of the fish.

    Science.gov (United States)

    Tohyama, Y; Kasama-Yoshida, H; Sakuma, M; Kobayashi, Y; Cao, Y; Hasegawa, M; Kojima, H; Tamai, Y; Tanokura, M; Kurihara, T

    1999-07-01

    The structure of Latimeria chalumnae (coelacanth) proteolipid protein/DM20 gene excluding exon 1 was determined, and the amino acid sequence of Latimeria DM20 corresponding to exons 2-7 was deduced. The nucleotide sequence of exon 3 suggests that only DM20 isoform is expressed in Latimeria. The structure of proteolipid protein/DM20 gene is well preserved among human, dog, mouse, and Latimeria. Southern blot analysis indicates that Latimeria DM20 gene is a single-copy gene. When the amino acid sequences of DM20 were compared among various species, Latimeria was more similar to tetrapods than other fishes including lungfish, confirming the previous finding by immunoreactivity (Waehneldt and Malotka 1989 J. Neurochem. 52:1941-1943). However, when phylogenetic trees were constructed from the DM20 sequences, lungfish was clearly the closest to tetrapods. Latimeria was situated outside of lungfish by the maximum likelihood method. The apparent similarity of Latimeria DM20 to tetrapod proteolipid protein/DM20 is explained by the slow amino acid substitution rate of Latimeria DM20.

  9. Convergent myotonic dystrophy (DM) haplotypes on 19q13.3: Potential inconsistencies in human disease gene localization

    Energy Technology Data Exchange (ETDEWEB)

    Tsilfidis, C. [Eye Research Institute of Canada, Toronto (Canada); Whiting, E.J. [Univ. of Ottawa (Canada); Korneluk, R.G. [Univ. of Ottawa (Canada)]|[Childrens Hospital of Eastern Ontario, Ottawa (Canada)

    1994-09-01

    Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease which has been shown to be caused by an unstable trinucleotide repeat located on chromosome 19q. We have conducted extensive haplotype analysis on 103 DM chromosome using thirteen 19q13.3 loci identifying 18 RFLPs, spanning a physical distance of 1.3 Mb containing the myotonic dystrophy gene. Three major haplotypes, H1, H2 and H3, comprising 45.6% of the DM chromosomes, were observed in our population. The later two haplotypes, observed exclusively on DM chromosomes of French Canadian origin, contain a 500 kb core region that is identical. The low frequency of this core on normal chromosomes (0.8%) is consistent with a mapping of the DM gene within this region. However, the DM mutation is found 160 kb distal to the point of divergence between these two haplotypes. In contrast, the 450 kb shared by haplotypes H1 and H2 contain the DM mutation. Further, analysis of the DM region using a polymorphic microsatellite GJ-VSSM2 located 15 kb telomeric to the DM gene revealed strong allelic association of allele V on DM chromosomes (present on 6% of normal and 88.2% of DM chromosomes). The fact that allele V was found on all DM chromosomes with the three major haplotypes is indicative of their common origin and includes the two French Canadian haplotypes which share a region proximal to the DM mutation. This analysis indicates that convergent haplotypes, in the absence of a more extensive linkage disequilibrium analysis, may lead to a spurious disease gene localization.

  10. Data of evolutionary structure change: 1AIJL-2J8DM [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AIJL-2J8DM 1AIJ 2J8D L M -------------------ALLSFERKYRVPGGTLV--G...ment> 0 1AIJ L 1AIJ...788360596 8.022679328918457 1 1AIJ... L 1AIJL YGLGG-APLAK...line> GLU CA 293 ARG CA 280 1AIJ

  11. Discovery of Low DM Fast Radio Transients: Geminga Pulsar Caught in the Act

    CERN Document Server

    Maan, Yogesh

    2015-01-01

    We report discovery of several energetic radio bursts at 34 MHz, using the Gauribidanur radio telescope. The radio bursts exhibit two important properties associated with the propagation of astronomical signals through the interstellar medium: (i) frequency dependent dispersive delays across the observing bandwidth, and (ii) Faraday rotation of the plane of linear polarization. These bursts sample a range of dispersion measures (DM; 1.4--3.6$~{\\rm pc}~{\\rm cm}^{-3}$), and show DM-variation at timescales of the order of a minute. Using groups of bursts having a consistent DM, we show that the bursts have originated from the radio-quiet gamma-ray pulsar Geminga. Detection of these bursts supports the existence of occasional radio emission from Geminga. The rare occurrence of these bursts, and the short timescale variation in their DM (if really caused by the intervening medium or the pulsar magnetosphere), might provide clues as to why the pulsar has not been detected in earlier sensitive searches. We present d...

  12. Does the diffusion DM-DE interaction model solve cosmological puzzles?

    CERN Document Server

    Szydlowski, Marek

    2016-01-01

    We study dynamics of cosmological models with diffusion effects modeling dark matter and dark energy interactions. We show the simple model with diffusion between the cosmological constant sector and dark matter, where the canonical scaling law of dark matter $(\\rho_{dm,0}a^{-3}(t))$ is modified by an additive $\\epsilon(t)=\\gamma t a^{-3}(t)$ to the form $\\rho_{dm}=\\rho_{dm,0}a^{-3}(t)+\\epsilon(t)$. We reduced this model to the autonomous dynamical system and investigate it using dynamical system methods. This system possesses a two-dimensional invariant submanifold on which the DM-DE interaction can be analyzed on the phase plane. The state variables are density parameter for matter (dark and visible) and parameter $\\delta$ characterizing the rate of growth of energy transfer between the dark sectors. A corresponding dynamical system belongs to a general class of jungle type of cosmologies represented by coupled cosmological models in a Lotka-Volterra framework. We demonstrate that the de Sitter solution is ...

  13. Non-chiral fusion rules, structure constants of $D_{m}$ minimal models

    CERN Document Server

    Rida, A

    1999-01-01

    We present a technique to construct, for $D_{m}$ unitary minimal models, the non-chiral fusion rules which determines the operator content of the operator product algebra. Using these rules we solve the bootstrap equations and therefore determine the structure constants of these models. Through this approach we emphasize the role played by some discrete symmetries in the classification of minimal models.

  14. AMS02 positron excess from decaying fermion DM with local dark gauge symmetry

    Directory of Open Access Journals (Sweden)

    P. Ko

    2015-02-01

    Full Text Available Positron excess observed by PAMELA, Fermi and AMS02 may be due to dark matter (DM pair annihilation or decay dominantly into muons. In this paper, we consider a scenario with thermal fermionic DM (χ with mass ∼O(1–2 TeV decaying into a dark Higgs (ϕ and an active neutrino (νa instead of the SM Higgs boson and νa. We first present a renormalizable model for this scenario with local dark U(1X gauge symmetry, in which the DM χ can be thermalized by Higgs portal and the gauge kinetic mixing. Assuming the dark Higgs (ϕ mass is in the range 2mμDM can be enhanced to some extent, and three puzzles in the CDM paradigm can be somewhat relaxed.

  15. Effect of DM Actuator Errors on the WFIRST/AFTA Coronagraph Contrast Performance

    Science.gov (United States)

    Sidick, Erkin; Shi, Fang

    2015-01-01

    The WFIRST/AFTA 2.4 m space telescope currently under study includes a stellar coronagraph for the imaging and the spectral characterization of extrasolar planets. The coronagraph employs two sequential deformable mirrors (DMs) to compensate for phase and amplitude errors in creating dark holes. DMs are critical elements in high contrast coronagraphs, requiring precision and stability measured in picometers to enable detection of Earth-like exoplanets. Working with a low-order wavefront-sensor the DM that is conjugate to a pupil can also be used to correct low-order wavefront drift during a scientific observation. However, not all actuators in a DM have the same gain. When using such a DM in low-order wavefront sensing and control subsystem, the actuator gain errors introduce high-spatial frequency errors to the DM surface and thus worsen the contrast performance of the coronagraph. We have investigated the effects of actuator gain errors and the actuator command digitization errors on the contrast performance of the coronagraph through modeling and simulations, and will present our results in this paper.

  16. Studies on the s_dm.t=f verb form in Classical Egyptian

    NARCIS (Netherlands)

    Zonhoven, Ludovicus Martinus Johannes

    1997-01-01

    This study is devoted to some synchronic aspects of the sDm.t=f verb form, primarily its meaning and uses in Classical Egyptian. In the introduction some attention is paid to the history of the studies of the form and its origin, an aspect which will receive no further consideration. In accordance

  17. Studies on the s_dm.t=f verb form in Classical Egyptian

    NARCIS (Netherlands)

    Zonhoven, Ludovicus Martinus Johannes

    1997-01-01

    This study is devoted to some synchronic aspects of the sDm.t=f verb form, primarily its meaning and uses in Classical Egyptian. In the introduction some attention is paid to the history of the studies of the form and its origin, an aspect which will receive no further consideration. In accordance w

  18. Studies on the s_dm.t=f verb form in Classical Egyptian

    NARCIS (Netherlands)

    Zonhoven, Ludovicus Martinus Johannes

    1997-01-01

    This study is devoted to some synchronic aspects of the sDm.t=f verb form, primarily its meaning and uses in Classical Egyptian. In the introduction some attention is paid to the history of the studies of the form and its origin, an aspect which will receive no further consideration. In accordance w

  19. Lipoprotein (a, C-reactive protein and some metabolic cardiovascular risk factors in type 2 DM

    Directory of Open Access Journals (Sweden)

    Ogbera Anthonia O

    2010-07-01

    Full Text Available Abstract Background Lipoprotein (a (LP (a is an independent cardiovascular risk factor that is not widely studied in people of sub-Saharan African origin. The aim of this report is to determine the frequency of occurrence of elevated Lp (a and possible relationship with total cholesterol (TCHOL, high density lipoprotein cholesterol (HDL-C, low density lipoprotein cholesterol (LDL-C, triglycerides (TG, C reactive protein (CRP and serum uric acid (SUA. Methods This is a cross sectional study carried out in 200 Nigerian patients with type 2 DM and 100 sex and age matched healthy Controls aged between 32-86 years. We determined the frequency of occurrence of elevated Lp (a levels in the study subjects and compared clinical and biochemical variables between type 2 diabetic patients and non-diabetic patients. Clinical and biochemical parameters were also compared between subjects with type 2 DM who had elevated LP (a and normal LP (a levels. Long term glycaemic control using glycosylated haemoglobin was determined and compared in the study subjects. Test statistics used include chi square, correlation coefficient analysis and Student's t test. Results The mean Lp(a concentration differed significantly between type 2 diabetic patients and the Control subjects (18.7 (5.8 mg/dl vs 23 (6.8 mg/dl, 0.00001. Similarly, the prevalence of high LP (a levels in type 2 DM patients was significantly higher than that of the Control subjects (12.5% vs 4%, p-0.019. The mean levels of the lipid profile parameters (TCHOL, LDL-C, TG, LDL/HDL and CRP were significantly higher in DM patients than in the Control subjects. The mean LP (a levels were comparable in both sexes and in DM subjects with and without hypertension. TG was the only parameter that differed significantly between subjects with elevated Lp (a levels and those with normal Lp (a levels. There was a significant positive correlation (r between Lp(a levels and TG, LDL-C. TCHOL, LDL/HDL and uric acid. No

  20. The Design of VoIP Terminal Device Based on DM365%基于DM365的IP语音终端设计

    Institute of Scientific and Technical Information of China (English)

    徐海潮; 张江鑫; 蒋伊乐

    2013-01-01

    This paper presents the whole hardware and software framework of the VoIP portable device based on DM365 , focusing on power management , voice codec module and wireless module .It mainly explains the local part of recording .This terminal device has the function of recording , supporting G .711 , storage and playback .It can be used for voice communications via 3 G or Wi-Fi combined with network transmission proto-col.%该文给出了基于DM365的便携式IP语音终端的整体硬件和软件框架,重点阐述了电源管理、语音编解码模块与无线模块等硬件设计,主要讲解了本地录音部分。该终端可以实现语音的录音,G711编解码,存储与回放,可在3G 与 WiFi 间自由切换,结合网络传输协议可用于语音通信。

  1. 基于DM642的嵌入式虹膜识别系统设计%Design of embedded iris identification system based on DM642

    Institute of Scientific and Technical Information of China (English)

    黄健; 沈建新

    2012-01-01

    提出一种基于TMS320DM642的嵌入式虹膜识别系统设计方案.该系统集图像采集、图像处理以及图像识别于一体,采用DSP/BIOS嵌入式操作系统,实现对虹膜图像的实时处理.对归一化的虹膜图像采用2D-Gabor滤波器实现特征提取,通过比较海明距实现编码匹配.实验表明,该系统具有识别准确率高、体积小、功耗低以及可靠性高等优点.%In this paper, a kind of embedded iris identification system based on DM642 is put forward. This system has image acquisition, image processing and image recognition .The software system is developed with DSP/BIOS embedded operating system to realize the real-time processing of iris image. With 2D - Gabor filter, the feature of normalized iris images is extracted, and the code is matched by hamming distance. Experiment results show that this system can run efficiently and stalility, which has high i-dentification accuracy, small size and low power consumption.

  2. The DM-QIM Digital Image Watermarking Algorithm Based on DCT Transformation%基于DCT变换下的DM-QIM数字图像水印算法

    Institute of Scientific and Technical Information of China (English)

    陈燕

    2013-01-01

    基于DCT域下的水印算法相对于空域中水印算法具有更好的稳定性,更大的容量,以及更好的隐蔽性,同时在借助人类的感知模型的情况下能设计出具有较好保真度的水印系统。本论文对DM-QIM(Quantization Index Modulation)水印方案进行了系统的研究,介绍了这种数字水印算法的原理及模型,探究了数字水印的嵌入和提取方案,最后对实验结果做了分析和总结。%DCT-based watermarking algorithm with respect to the airspace watermarking algorithm has strong robustness, and larger capacity, better concealment. While using human perception model can design better fidelity watermarking system. This chapter examines a DM-QIM (Quantization Index Modulation) watermarking scheme, describes the principle of the digital watermarking algorithm and model to analyze the digital watermark embedding and extraction program, finally analyzes and summarizes the experimental results.

  3. Technical improvements in the DNA analysis of the myotonic dystrophy (DM) mutation

    Energy Technology Data Exchange (ETDEWEB)

    Leblond, S.; Lehev, D.; Barcelo, J. [Children`s Hospital of Eastern Ontario, Ottawa (Canada)] [and others

    1994-09-01

    It has become increasingly clear that widespread clinical application of routine DNA diagnosis requires robust and easily replicated methodologies. Mutation analysis in DM involves detection of a CTG expansion which may increase in size between generations within a family. DNA testing has required two distinct methods: genomic and PCR DNA Southern blotting. Genomic Southerns visualize from E1 (hundreds of repeats) to the very largest E4 (thousands of repeats in congenital DM). PCR Southerns permit detection of the smallest mutations (E0, protomutations associated with minimal if any clinical signs) to E3, but E4 is not uniformly visualized. In order to improve the DM testing such that even the largest expansions are visualized by a single PCR test, we have altered the PCR conditions. Since the PCR conditions do not substantially affect the normal allele of less than 200 bp, CTG expansion must be directly monitored by hybridization with a labelled (CTG){sub 10} oligonucleotide. Unlike PCR of the CGG expansion in fragile X, addition of deazaGTP reduced visualization of the DM expansion. Addition of single-stranded protein and DMSO significantly improved PCR up to ten-fold such that E4s were visualized. The CTG expansion was very sensitive to the denaturing cycle temperature (which does not affect the intensity of the normal allele). Thus, 96{degrees}C on the Perkin Elmer 480 was optimal in our hands, with 98{degrees}C and 94{degrees}C actually causing loss of even the intermediate sized E1 and E2 expansions. This has implications when setting up the DM test on different thermocyclers where digital readings may not reflect actual block temperature. These PCR `tune-ups` will support more reliable and streamlined analyses, as more expansion mutations are recognized and routinely offered for clinical use.

  4. Technology

    Directory of Open Access Journals (Sweden)

    Xu Jing

    2016-01-01

    Full Text Available The traditional answer card reading method using OMR (Optical Mark Reader, most commonly, OMR special card special use, less versatile, high cost, aiming at the existing problems proposed a method based on pattern recognition of the answer card identification method. Using the method based on Line Segment Detector to detect the tilt of the image, the existence of tilt image rotation correction, and eventually achieve positioning and detection of answers to the answer sheet .Pattern recognition technology for automatic reading, high accuracy, detect faster

  5. YB-DM-3有机玻璃的疲劳性能研究%Study Fatigue Property of YB-DM-3 Perspex

    Institute of Scientific and Technical Information of China (English)

    马丽婷; 陈新文; 厉蕾

    2011-01-01

    YB-DM-3有机玻璃因其具有良好的透光性和力学性能,目前在飞机上已经得到广泛应用,它是一种区别于金属材料的高分子材料,通过对有机玻璃拉伸疲劳性能的测试得到了疲劳S-N曲线和条件疲劳极限;比较了不同应力比下的有机玻璃拉伸疲劳S-N曲线,随着应力比的增加曲线整体上移.%Perspex have light permeability and mechanical performance, and its application on aircraft is high molecular material of metallic material, test property fatigue of tensile, the perspex S-N curve of tensile fatigue was present in this research.

  6. 基于DM3730平台的智能数字视频监控系统%Intelligent Digital Video Surveillance System Based on DM3730

    Institute of Scientific and Technical Information of China (English)

    周建平; 刘歆浏

    2012-01-01

    Aiming at disadvantages of traditional PC platform monitoring system, such as high system cost, low calculating ability, simple functions, large size, complex operation, and limited usages range, design and implement an intelligent digital control system taking TI company is DM3730 as core. System uses a modular design, a Linux operating system, expansion of a variety of display outputs, configured with CMOS image sensors, Gigabit Ethernet interface module for automatic analysis of video streaming, motion detection, identification, tracking and early warning threat targets, and use Ethernet video compression and transmission. It has the advantages of low cost, high reliability, low false alarm rate.%针对传统PC平台的监控系统成本高、运算能力低、功能单一、体积大、操作复杂、使用范围受限的问题,设计及实现了一款以TI公司的DM3730为核心的智能数字监控系统.该监控系统采用模块化设计,以Linux为操作系统,扩展有多种显示输出接口,配置标准CMOS图像传感器,千兆以太网口等模块.该系统能自动分析视频流,主动检测、识别、跟踪及预警威胁目标,并用以太网实现视频的压缩传输,具备低成本、可靠性高、虚警率低的特点.

  7. Resistance to Downy Mildew in Lettuce 'La Brillante' is Conferred by Dm50 Gene and Multiple QTL.

    Science.gov (United States)

    Simko, Ivan; Ochoa, Oswaldo E; Pel, Mathieu A; Tsuchida, Cayla; Font I Forcada, Carolina; Hayes, Ryan J; Truco, Maria-Jose; Antonise, Rudie; Galeano, Carlos H; Michelmore, Richard W

    2015-09-01

    Many cultivars of lettuce (Lactuca sativa L.) are susceptible to downy mildew, a nearly globally ubiquitous disease caused by Bremia lactucae. We previously determined that Batavia type cultivar 'La Brillante' has a high level of field resistance to the disease in California. Testing of a mapping population developed from a cross between 'Salinas 88' and La Brillante in multiple field and laboratory experiments revealed that at least five loci conferred resistance in La Brillante. The presence of a new dominant resistance gene (designated Dm50) that confers complete resistance to specific isolates was detected in laboratory tests of seedlings inoculated with multiple diverse isolates. Dm50 is located in the major resistance cluster on linkage group 2 that contains at least eight major, dominant Dm genes conferring resistance to downy mildew. However, this Dm gene is ineffective against the isolates of B. lactucae prevalent in the field in California and the Netherlands. A quantitative trait locus (QTL) located at the Dm50 chromosomal region (qDM2.2) was detected, though, when the amount of disease was evaluated a month before plants reached harvest maturity. Four additional QTL for resistance to B. lactucae were identified on linkage groups 4 (qDM4.1 and qDM4.2), 7 (qDM7.1), and 9 (qDM9.2). The largest effect was associated with qDM7.1 (up to 32.9% of the total phenotypic variance) that determined resistance in multiple field experiments. Markers identified in the present study will facilitate introduction of these resistance loci into commercial cultivars of lettuce.

  8. Dynamics of the diffusive DM-DE interaction--dynamical system approach

    CERN Document Server

    Haba, Zbigniew; Szydlowski, Marek

    2016-01-01

    We discuss dynamics of a model of an energy transfer between dark energy (DE) and dark matter (DM). The energy transfer is determined by a non-conservation law resulting from a diffusion of dark matter in an environment of dark energy. The relativistic invariance defines the diffusion in a unique way. The system can contain baryonic matter and radiation which do not interact with the dark sector. We treat the Friedman equation and the conservation laws as a closed dynamical system. The dynamics of the model is examined using the dynamical systems methods for demonstration how solutions depend on initial conditions. We also fit the model parameters using astronomical observation: SNIa, $H(z)$, BAO and Alcock-Paczynski test. We show that the model with diffuse DM-DE is consistent with the data.

  9. Role of miRNAs in Epicardial Adipose Tissue in CAD Patients with T2DM

    Directory of Open Access Journals (Sweden)

    Yang Liu

    2016-01-01

    Full Text Available Background. Epicardial adipose tissue (EAT is identified as an atypical fat depot surrounding the heart with a putative role in the involvement of metabolic disorders, including obesity, type-2 diabetes mellitus, and atherosclerosis. We profiled miRNAs in EAT of metabolic patients with coronary artery disease (CAD and type-2 diabetes mellitus (T2DM versus metabolically healthy patients by microarray. Compared to metabolically healthy patients, we identified forty-two miRNAs that are differentially expressed in patients with CAD and T2DM from Xinjiang, China. Eleven miRNAs were selected as potential novel miRNAs according to P value and fold change. Then the potential novel miRNAs targeted genes were predicted via TargetScan, PicTar, and miRTarbase, and the function of the target genes was predicted via Gene Ontology (GO analysis while the enriched KEGG pathway analyses of the miRNAs targeted genes were performed by bioinformatics software DAVID. Then protein-protein interaction networks of the targeted gene were conducted by online software STRING. Finally, using microarray, bioinformatics approaches revealed the possible molecular mechanisms pathogenesis of CAD and T2DM. A total of 11 differentially expressed miRNAs were identified and among them, hsa-miR-4687-3p drew specific attention. Bioinformatics analysis revealed that insulin signaling pathway is the central way involved in the progression of metabolic disorders. Conclusions. The current findings support the fact that miRNAs are involved in the pathogenesis of metabolic disorders in EAT of CAD patients with T2DM, and validation of the results of these miRNAs by independent and prospective study is certainly warranted.

  10. Protoplanetary disk formation and evolution models: DM Tau and GM Aur

    Science.gov (United States)

    Hueso, R.; Guillot, T.

    2002-09-01

    We study the formation and evolution of protoplanetary disks using an axisymmetric turbulent disk model. We compare model results with observational parameters derived for the DM Tau and GM Aur systems. These are relatively old T Tauri stars with large and massive protoplanetary disks. Early disk formation is studied in the standard scenario of slowly rotating isothermal collapsing spheres and is strongly dependent on the initial angular momentum and the collapse accretion rate. The viscous evolution of the disk is integrated in time using the classical Alpha prescription of turbulence. We follow the temporal evolution of the disks until their characteristics fit the observed characteristics of DM Tau and GM Aur. We therefore obtain the set of model parameters that are able to explain the present state of these disks. We also study the disk evolution under the Beta parameterization of turbulence, recently proposed for sheared flows on protoplanetary disks. Both parameterizations allow explaining the present state of both DM Tau and GM Aur. We infer a value of Alpha between 5x10-3 to 0.02 for DM Tau and one order of magnitude smaller for GM Aur. Values of the Beta parameter are in accordance with theoretical predictions of Beta around 2x10-5 but with a larger dispersion on other model parameters, which make us favor the Alpha parameterization of turbulence. Implications for planetary system development in these systems are presented. In particular, GM Aur is a massive and slowly evolving disk where conditions are very favorable for planetesimal growth. The large value of present disk mass and the relatively small observed accretion rate of this system may also be indicative of the presence of an inner gas giant planet. Acknowledgements: This work has been supported by Programme Nationale de Planetologie. R. Hueso acknowledges a post-doctoral fellowship from Gobierno Vasco.

  11. DD-08PHASE I CANCER CLINICAL TRIAL FOR 4-DEMETHYL-4-CHOLESTERYLOXYCARBONYLPENCLOMEDINE (DM-CHOC-PEN)

    Science.gov (United States)

    Ware, Marcus; Weiner, Roy; Friedlander, Paul; Gordon, Crag; Saenger, Yvonne; Mahmood, Tallat; Rodgers, Andrew; Bastian, Gerald; Urien, S.; Lee; Morgan, Roy

    2014-01-01

    PURPOSE: DM-CHOC-PEN is a poly-chlorinated pyridine cholesteryl carbonate whose MOA is via alkylation of DNA @ N7 – guanine and via oxidative stress. The aims of this clinical trial were to determine maximum-tolerated dose (MTD), safety, dose-limiting toxicities (DLTs), pharmacokinetics (PK) of DM-CHOC-PEN and monitor for clinical responses. METHODS: DM-CHOC-PEN was administered as a 3-hr IV infusion once every 21-days to patients with advanced cancer; melanoma (n = 3), colorectal CA (n = 3), breast (n = 3) and glioblastoma multiforme (n = 6). The trial included patients with advanced cancer +/- CNS involvement. The starting dose was 39 mg/m2 with escalations to date up to 111 mg/m2. RESULTS: Twenty-six (26) patients have been treated. The MTD was 2-tiered and defined as 85.8 mg/m2 for patients with liver involvement and 98.7 mg/m2 for patients without liver abnormalities. The most common adverse effects were fatigue (n = 2), liver dysfunction – elevated bilirubin (Gr-3, n = 3; Gr-2, n = 1), ALT/AST (Gr-2, n = 3), alk phos (Gr-2, n = 3) and an allergic reaction (Gr-2, n = 1). Three (3) patients with liver metastasis demonstrated hyperbilirubinemia (Gr-3 SLT) – 2 at the 98.7 mg/m2 and one (1) at the 111 mg/m2 levels Five (5) additional patients with liver disease have been treated at 85.8 mg/m2 level without toxicity. CONCLUSIONS: DM-CHOC-PEN is safe at the presented dose levels and has a favorable PK profile. Eight (8) patients had responses or significant PFS, including 6 with CNS involvement. A Phase II trial has begun in patients with primary brain cancer and brain metastases from melanoma, breast cancer and lung cancer.

  12. Sensitive limits on the abundance of cold water vapor in the DM Tauri protoplanetary disk

    NARCIS (Netherlands)

    Bergin, E.A.; Hogerheijde, M.R.; Brinch, C.; Fogel, J.; Yıldız, U.A.; Kristensen, L.E.; van Dishoeck, E.F.; Bell, T.A.; Blake, G.A.; Cernicharo, J.; Dominik, C.; Lis, D.; Melnick, G.; Neufeld, D.; Panić, O.; Pearson, J.C.; Bachiller, R.; Baudry, A.; Benedettini, M.; Benz, A.O.; Bjerkeli, P.; Bontemps, S.; Braine, J.; Bruderer, S.; Caselli, P.; Codella, C.; Daniel, F.; Di Giorgio, A.M.; Doty, S.D.; Encrenaz, P.; Fich, M.; Fuente, A.; Giannini, T.; Goicoechea, J.R.; de Graauw, T.; Helmich, F.; Herczeg, G.J.; Herpin, F.; Jacq, T.; Johnstone, D.; Jørgensen, J.K.; Larsson, B.; Liseau, R.; Marseille, M.; McCoey, C.; Nisini, B.; Olberg, M.; Parise, B.; Plume, R.; Risacher, C.; Santiago-García, J.; Saraceno, P.; Shipman, R.; Tafalla, M.; van Kempen, T.A.; Visser, R.; Wampfler, S.F.; Wyrowski, F.; van der Tak, F.; Jellema, W.; Tielens, A.G.G.M.; Hartogh, P.; Stützki, J.; Szczerba, R.

    2010-01-01

    We performed a sensitive search for the ground-state emission lines of ortho-and para-water vapor in the DM Tau protoplanetary disk using the Herschel/HIFI instrument. No strong lines are detected down to 3 sigma levels in 0.5 km s(-1) channels of 4.2 mK for the 1(10)-1(01) line and 12.6 mK for the

  13. TREX-DM: a low background Micromegas-based TPC for low-mass WIMP detection

    CERN Document Server

    Iguaz, F J; Aznar, F; Castel, J F; Cebrian, S; Dafni, T; Garcia, J A; Irastorza, I G; Lagraba, A; Luzon, G; Peiro, A

    2016-01-01

    Dark Matter experiments are recently focusing their detection techniques in low-mass WIMPs, which requires the use of light elements and low energy threshold. In this context, we describe the TREX-DM experiment, a low background Micromegas-based TPC for low-mass WIMP detection. Its main goal is the operation of an active detection mass $\\sim$0.3 kg, with an energy threshold below 0.4 keVee and fully built with previously selected radiopure materials. This work describes the commissioning of the actual setup situated in a laboratory on surface and the updates needed for a possible physics run at the Canfranc Underground Laboratory (LSC) in 2016. A preliminary background model of TREX-DM is also presented, based on a Geant4 simulation, the simulation of the detector's response and two discrimination methods: a conservative muon/electron and one based on a neutron source. Based on this background model, TREX-DM could be competitive in the search for low-mass WIMPs. In particular it could be sensitive, e.g., to t...

  14. Anti-listerial Bactericidal Activity of Lactobacillus plantarum DM5 Isolated from Fermented Beverage Marcha.

    Science.gov (United States)

    Das, Deeplina; Goyal, Arun

    2013-09-01

    The strain Lactobacillus plantarum DM5 was isolated from fermented beverage Marcha of Sikkim and explored for its antagonistic activity against food-borne pathogens. The cell-free supernatant of L. plantarum DM5 showed antibacterial activity of 6,400 AU/mL in MRS medium (pH 6.0) against the indicator strain Staphylococcus aureus. MRS medium supplemented with 15 g/L of maltose at 37 °C under static condition yielded highest antimicrobial activity (6,400 AU/mL) with 3 % increase in specific activity when compared to 20 g/L glucose. The antimicrobial compound was heat stable (60 min at 100 °C) and was active over a wide pH range. It showed bactericidal effect on S. aureus and Listeria monocytogenes by causing 96 and 98 % of cell lysis, respectively. The cell morphology of the treated S. aureus and L. monocytogenes was completely deformed as revealed by scanning electron microscopy, suggesting the high potential of L. plantarum DM5 as natural preservatives in food industry. The antimicrobial compound was purified by 80 % ammonium sulphate precipitation and showed antimicrobial activity of 12,800 AU/mL with 19-fold purification and a molecular mass of 15.2 kDa, indicating the proteinaceous nature of the compound.

  15. Effect of Exercise on Psychological Well-being in T2DM

    Directory of Open Access Journals (Sweden)

    Farzad Najafipoor

    2011-09-01

    Full Text Available Background: Type 2 diabetic patients (T2DM experience health problems including psychiatric and psychological complications that influence their general health. Since exercise has an additional effect on psychological improvement, we aimed to establish the role of exercise as improvement of psychological problems. Methods: 80 subjects with T2DM were assigned to take exercise for 90 minutes per session, 3 times a week for a period of 4 months. They answered the GHQ-12 questionnaire before and after the study project. Results: Questionnaires were scored by Likert model and entered the statistical analysis. Our findings demonstrate a significant decrease in the mean GHQ-12 scores. [13.39 ± 5.89 to 8.52 ± 5.12 (p < 0.001]. Factor analysis by Graetz's three-factor model suggests that factor I (anxiety and depression associates with more improvement than the other factors.Conclusion: Exercise improves psychological distress in T2DM and results in improved well-being.

  16. Fault-tolerant drive electronics for a Xinetics deformable mirror at GeMS DM0

    Science.gov (United States)

    Barberio, Michael J.

    2016-07-01

    Gemini South is replacing one of the (3) CILAS DMs with a 349-actuator Xinetics DM in its GeMS MCAO system. Xinetics mirrors operate over a 40-100V dynamic range and require that inter-actuator stroke differences are limited to half-scale; each actuator must be within 30V of its neighbor to prevent mechanical stress and possible face sheet separation. A robust way to implement this protection is to use high power transient voltage suppressors (TVSs) as a 2D-mesh between the amplifiers and mirror, but this has system implications. A sustained clamp condition dissipates significant power in the devices, and if an actuator fails as short (which occurred once with the DM in a thermal chamber), the system is subject to a cascade failure event as multiple outputs drive the shorted actuator through the TVS network. This latter risk is readily resolved by using series fuses to the DM. In this third-generation driver, current sensing and logic inhibit amplifier outputs after a sustained TVS clamp condition or shorted output, and LED indicators show the location. Redundant thermal sensing is used on modular TVS row and column boards. A second 2D-mesh of high impedance resistors after the fuses will hold an unpowered channel to the average voltage of its neighbors, with a negligible influence function. A Failure Modes and Effects Analysis shows significant fault tolerance.

  17. Polyuria with the Concurrent manifestation of Central Diabetes Insipidus (CDI) & Type 2 Diabetes Mellitus (DM).

    Science.gov (United States)

    Shin, Hyun-Jong; Kim, Jae-Ha; Yi, Joo-Hark; Han, Sang-Woong; Kim, Ho-Jung

    2012-12-01

    We report a rare case of the concurrent manifestation of central diabetes insipidus (CDI) and type 2 diabetes mellitus (DM). A 56 year-old man was diagnosed as a type 2 DM on the basis of hyperglycemia with polyuria and polydipsia at a local clinic two months ago and started an oral hypoglycemic medication, but resulted in no symptomatic improvement at all. Upon admission to the university hospital, the patient's initial fasting blood sugar level was 140 mg/dL, and he showed polydipsic and polyuric conditions more than 8 L urine/day. Despite the hyperglycemia controlled with metformin and diet, his symptoms persisted. Further investigations including water deprivation test confirmed the coexisting CDI of unknown origin, and the patient's symptoms including an intense thirst were markedly improved by desmopressin nasal spray (10 µg/day). The possibility of a common origin of CDI and type 2 DM is raised in a review of the few relevant adult cases in the literature.

  18. Assessing Cognitive Processes with Diffusion Model Analyses: A Tutorial based on fast-dm-30

    Directory of Open Access Journals (Sweden)

    Andreas eVoss

    2015-03-01

    Full Text Available Diffusion models can be used to infer cognitive processes involved in fast binary decision tasks. The model assumes that information is accumulated continuously until one of two thresholds is hit. In the analysis, response time distributions from numerous trials of the decision task are used to estimate a set of parameters mapping distinct cognitive processes. In recent years, diffusion model analyses have become more and more popular in different fields of psychology. This increased popularity is based on the recent development of several software solutions for the parameter estimation. Although these programs make the application of the model relatively easy, there is a shortage of knowledge about different steps of a state-of-the-art diffusion model study. In this paper, we give a concise tutorial on diffusion modelling, and we present fast-dm-30, a thoroughly revised and extended version of the fast-dm software (Voss & Voss, 2007 for diffusion model data analysis. The most important improvement of the fast-dm version is the possibility to choose between different optimization criteria (i.e., Maximum Likelihood, Chi-Square, and Kolmogorov-Smirnov, which differ in applicability for different data sets.

  19. Insulin initiation and intensification in patients with T2DM for the primary care physician

    Directory of Open Access Journals (Sweden)

    Unger J

    2011-06-01

    Full Text Available Jeff UngerCatalina Research Institute, Chino, CA, USAAbstract: Type 2 diabetes mellitus (T2DM is characterized by both insulin resistance and inadequate insulin secretion. All patients with the disease require treatment to achieve and maintain the target glycosylated hemoglobin (A1C level of 6.5%–7%. Pharmacological management of T2DM typically begins with the introduction of oral medications, and the majority of patients require exogenous insulin therapy at some point in time. Primary care physicians play an essential role in the management of T2DM since they often initiate insulin therapy and intensify regimens over time as needed. Although insulin therapy is prescribed on an individualized basis, treatment usually begins with basal insulin added to a background therapy of oral agents. Prandial insulin injections may be added if glycemic targets are not achieved. Treatments may be intensified over time using patient-friendly titration algorithms. The goal of insulin intensification within the primary care setting is to minimize patients' exposure to chronic hyperglycemia and weight gain, and reduce patients' risk of hypoglycemia, while achieving individualized fasting, postprandial, and A1C targets. Simplified treatment protocols and insulin delivery devices allow physicians to become efficient prescribers of insulin intensification within the primary care arena.Keywords: diabetes, basal, bolus, regimens, insulin analogs, structured glucose testing

  20. Intensified glucose self-monitoring with education in Saudi DM patients.

    Science.gov (United States)

    Ba-Essa, Ebtesam M; Mobarak, Eman I; Alghamdi, Abdulaziz; Al-Daghri, Nasser M

    2015-01-01

    The purpose of this study is to assess the impact of intensified SMBG with patient education on DM patients at the Eastern province of Saudi Arabia. 60 poorly controlled adult type 1 and 2 DM patients (30 intervention; 30 control) were included in this 4-month case-control study. All patients were subjected to the same educational program at baseline. Controls were followed up after 3 months. The intervention group was followed monthly. Fasting blood glucose, HbA1c and lipid profile levels were the main outcome measures. The intervention arm showed significant reduction in the post-fasting glucose (P<0.001) and HbA1c (P<0.001) levels as well as a significant increase in glucose testing (P<0.001) than pre-levels. Both post-fasting glucose and HbA1c levels were significantly lower in the intervention arm than the control arm (P<0.001 and P=0.001, respectively). The intervention group also showed higher improvement in knowledge, attitude and behavior than the controls (P<0.001). Short duration of structured periodic SMBG with patient education significantly improved glycemic control in all DM patients, regardless of the type or mode of treatment. It facilitated timely and aggressive treatment modification and encouraged patient self-care behavior.

  1. Curcumin analog DM-1 in monotherapy or combinatory treatment with dacarbazine as a strategy to inhibit in vivo melanoma progression.

    Directory of Open Access Journals (Sweden)

    Fernanda Faião-Flores

    Full Text Available Malignant melanoma is a highly aggressive form of skin cancer with a high mortality rate if not discovered in early stages. Although a limited number of treatment options for melanoma currently exist, patients with a more aggressive form of this cancer frequently decline treatment. DM-1 is a sodium phenolate and curcumin analog with proven anticancer, anti-proliferative and anti-metastatic properties. In this paper, the DM-1 compound showed in vivo antitumor activity alone or in combination with chemotherapeutic DTIC in B16F10 melanoma-bearing mice. Beneficial effects such as melanoma tumor burden reduction with pyknotic nuclei, decreased nuclei/cytoplasmic ratio and nuclear degradation occurred after DM-1 treatment. No toxicological changes were observed in the liver, kidneys, spleen and lungs after DM-1 monotherapy or DTIC combined therapy. DTIC+DM-1 treatment induced the recovery of anemia arising from melanoma and immunomodulation. Both DM-1 treatment alone and in combination with DTIC induced apoptosis with the cleavage of caspase-3, -8 and -9. Furthermore, melanoma tumors treated with DM-1 showed a preferential apoptotic intrinsic pathway by decreasing Bcl-2/Bax ratio. Considering the chemoresistance exhibited by melanoma towards conventional chemotherapy drugs, DM-1 compound in monotherapy or in combination therapy provides a promising improvement in melanoma treatment with a reduction of side effects.

  2. Curcumin Analog DM-1 in Monotherapy or Combinatory Treatment with Dacarbazine as a Strategy to Inhibit In Vivo Melanoma Progression

    Science.gov (United States)

    Faião-Flores, Fernanda; Quincoces Suarez, José Agustín; Fruet, Andréa Costa; Maria-Engler, Silvya Stuchi; Pardi, Paulo Celso; Maria, Durvanei Augusto

    2015-01-01

    Malignant melanoma is a highly aggressive form of skin cancer with a high mortality rate if not discovered in early stages. Although a limited number of treatment options for melanoma currently exist, patients with a more aggressive form of this cancer frequently decline treatment. DM-1 is a sodium phenolate and curcumin analog with proven anticancer, anti-proliferative and anti-metastatic properties. In this paper, the DM-1 compound showed in vivo antitumor activity alone or in combination with chemotherapeutic DTIC in B16F10 melanoma-bearing mice. Beneficial effects such as melanoma tumor burden reduction with pyknotic nuclei, decreased nuclei/cytoplasmic ratio and nuclear degradation occurred after DM-1 treatment. No toxicological changes were observed in the liver, kidneys, spleen and lungs after DM-1 monotherapy or DTIC combined therapy. DTIC+DM-1 treatment induced the recovery of anemia arising from melanoma and immunomodulation. Both DM-1 treatment alone and in combination with DTIC induced apoptosis with the cleavage of caspase-3, -8 and -9. Furthermore, melanoma tumors treated with DM-1 showed a preferential apoptotic intrinsic pathway by decreasing Bcl-2/Bax ratio. Considering the chemoresistance exhibited by melanoma towards conventional chemotherapy drugs, DM-1 compound in monotherapy or in combination therapy provides a promising improvement in melanoma treatment with a reduction of side effects. PMID:25742310

  3. Structural characterization of the maytansinoid–monoclonal antibody immunoconjugate, huN901–DM1, by mass spectrometry

    National Research Council Canada - National Science Library

    Wang, Lintao; Amphlett, Godfrey; Blättler, Walter A; Lambert, John M; Zhang, Wei

    2005-01-01

    ...‐specific drug delivery. The immunoconjugate, huN901–DM1, composed of the humanized monoclonal IgG 1 antibody, huN901, and the maytansinoid drug, DM1, is being tested in clinical trials to treat small cell lung carcinoma (SCLC). huN901...

  4. Cytoplasmic CUG RNA foci are insufficient to elicit key DM1 features.

    Directory of Open Access Journals (Sweden)

    Warunee Dansithong

    Full Text Available The genetic basis of myotonic dystrophy type I (DM1 is the expansion of a CTG tract located in the 3' untranslated region of DMPK. Expression of mutant RNAs encoding expanded CUG repeats plays a central role in the development of cardiac disease in DM1. Expanded CUG tracts form both nuclear and cytoplasmic aggregates, yet the relative significance of such aggregates in eliciting DM1 pathology is unclear. To test the pathophysiology of CUG repeat encoding RNAs, we developed and analyzed mice with cardiac-specific expression of a beta-galactosidase cassette in which a (CTG(400 repeat tract was positioned 3' of the termination codon and 5' of the bovine growth hormone polyadenylation signal. In these animals CUG aggregates form exclusively in the cytoplasm of cardiac cells. A key pathological consequence of expanded CUG repeat RNA expression in DM1 is aberrant RNA splicing. Abnormal splicing results from the functional inactivation of MBNL1, which is hypothesized to occur due to MBNL1 sequestration in CUG foci or from elevated levels of CUG-BP1. We therefore tested the ability of cytoplasmic CUG foci to elicit these changes. Aggregation of CUG RNAs within the cytoplasm results both in Mbnl1 sequestration and in approximately a two fold increase in both nuclear and cytoplasmic Cug-bp1 levels. Significantly, despite these changes RNA splice defects were not observed and functional analysis revealed only subtle cardiac dysfunction, characterized by conduction defects that primarily manifest under anesthesia. Using a human myoblast culture system we show that this transgene, when expressed at similar levels to a second transgene, which encodes expanded CTG tracts and facilitates both nuclear focus formation and aberrant splicing, does not elicit aberrant splicing. Thus the lack of toxicity of cytoplasmic CUG foci does not appear to be a consequence of low expression levels. Our results therefore demonstrate that the cellular location of CUG RNA

  5. A food additive with prebiotic properties of an α-d-glucan from lactobacillus plantarum DM5.

    Science.gov (United States)

    Das, Deeplina; Baruah, Rwivoo; Goyal, Arun

    2014-08-01

    An α-d-glucan produced by Lactobacillus plantarum DM5 was explored for in vitro prebiotic activities. Glucan-DM5 demonstrated 21.6% solubility, 316.9% water holding capacity, 86.2% flocculation activity, 71.4% emulsification activity and a degradation temperature (Td) of 292.2°C. Glucan-DM5 exhibited lowest digestibility of 0.54% by artificial gastric juice, 0.21% by intestinal fluid and 0.32% by α-amylase whereas the standard prebiotic inulin, showed 25.23%, 5.97% and 19.13%, hydrolysis, respectively. Prebiotic activity assay of glucan-DM5 displayed increased growth of probiotic bacteria such as Bifidobacterium infantis and Lactobacillus acidophilus, but did not support the growth of non-probiotic bacteria such as Escherichia coli and Enterobacter aerogenes. The overall findings indicated that glucan from L. plantarum DM5 can serve as a potential prebiotic additive for food products.

  6. High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions.

    Science.gov (United States)

    Hua, Yun; Strock, Christopher J; Johnston, Paul A

    2015-01-01

    This chapter describes the implementation of the p53-hDM2 protein-protein interaction (PPI) biosensor (PPIB) HCS assay to identify disruptors of p53-hDM2 PPIs. Recombinant adenovirus expression constructs were generated bearing the individual p53-GFP and hDM2-RFP PPI partners. The N-terminal p53 transactivating domain that contains the binding site for hDM2 is expressed as a GFP fusion protein that is targeted and anchored in the nucleolus of infected cells by a nuclear localization (NLS) sequence. The p53-GFP biosensor is localized to the nucleolus to enhance and facilitate the image acquisition and analysis of the PPIs. The N-terminus of hDM2 encodes the domain for binding to the transactivating domain of p53, and is expressed as a RFP fusion protein that includes both an NLS and a nuclear export sequence (NES). In U-2 OS cells co-infected with both adenovirus constructs, the binding interactions between hDM2 and p53 result in both biosensors becoming co-localized within the nucleolus. Upon disruption of the p53-hDM2 PPIs, the p53-GFP biosensor remains in the nucleolus while the shuttling hDM2-RFP biosensor redistributes into the cytoplasm. p53-hDM2 PPIs are measured by acquiring fluorescent images of cells co-infected with both adenovirus biosensors on an automated HCS imaging platform and using an image analysis algorithm to quantify the relative distribution of the hDM2-RFP shuttling component of the biosensor between the cytoplasm and nuclear regions of compound treated cells.

  7. Development of a pseudo phased array technique using EMATs for DM weld testing

    Energy Technology Data Exchange (ETDEWEB)

    Cobb, Adam C., E-mail: adam.cobb@swri.org; Fisher, Jay L., E-mail: adam.cobb@swri.org [Southwest Research Institute, Sensor Systems and Nondestructive Technology Department, 6220 Culebra Road, San Antonio, TX 78238-5166 (United States); Shiokawa, Nobuyuki; Hamano, Toshiaki; Horikoshi, Ryoichi; Ido, Nobukazu [IHI Corporation, Nuclear Power Operations, Yokohama Engineering Center, 1, Shin-Nakahara-cho, Isogo-ku, Yokohama 235-8501 (Japan)

    2015-03-31

    Ultrasonic inspection of dissimilar metal (DM) welds in piping with cast austenitic stainless steel (CASS) has been an area ongoing research for many years given its prevalence in the petrochemical and nuclear industries. A typical inspection strategy for pipe welds is to use an ultrasonic phased array system to scan the weld from a sensor located on the outer surface of the pipe. These inspection systems generally refract either longitudinal or shear vertical (SV) waves at varying angles to inspect the weld radially. In DM welds, however, the welding process can produce a columnar grain structure in the CASS material in a specific orientation. This columnar grain structure can skew ultrasonic waves away from their intended path, especially for SV and longitudinal wave modes. Studies have shown that inspection using the shear horizontal (SH) wave mode significantly reduces the effect of skewing. Electromagnetic acoustic transducers (EMATs) are known to be effective for producing SH waves in field settings. This paper presents an inspection strategy that seeks to reproduce the scanning and imaging capabilities of a commercial phase array system using EMATs. A custom-built EMAT was used to collect data at multiple propagation angles, and a processing strategy known as the synthetic aperture focusing technique (SAFT) was used to combine the data to produce an image. Results are shown using this pseudo phased array technique to inspect samples with a DM weld and artificial defects, demonstrating the potential of this approach in a laboratory setting. Recommendations for future work to transition the technique to the field are also provided.

  8. Development of a pseudo phased array technique using EMATs for DM weld testing

    Science.gov (United States)

    Cobb, Adam C.; Fisher, Jay L.; Shiokawa, Nobuyuki; Hamano, Toshiaki; Horikoshi, Ryoichi; Ido, Nobukazu

    2015-03-01

    Ultrasonic inspection of dissimilar metal (DM) welds in piping with cast austenitic stainless steel (CASS) has been an area ongoing research for many years given its prevalence in the petrochemical and nuclear industries. A typical inspection strategy for pipe welds is to use an ultrasonic phased array system to scan the weld from a sensor located on the outer surface of the pipe. These inspection systems generally refract either longitudinal or shear vertical (SV) waves at varying angles to inspect the weld radially. In DM welds, however, the welding process can produce a columnar grain structure in the CASS material in a specific orientation. This columnar grain structure can skew ultrasonic waves away from their intended path, especially for SV and longitudinal wave modes. Studies have shown that inspection using the shear horizontal (SH) wave mode significantly reduces the effect of skewing. Electromagnetic acoustic transducers (EMATs) are known to be effective for producing SH waves in field settings. This paper presents an inspection strategy that seeks to reproduce the scanning and imaging capabilities of a commercial phase array system using EMATs. A custom-built EMAT was used to collect data at multiple propagation angles, and a processing strategy known as the synthetic aperture focusing technique (SAFT) was used to combine the data to produce an image. Results are shown using this pseudo phased array technique to inspect samples with a DM weld and artificial defects, demonstrating the potential of this approach in a laboratory setting. Recommendations for future work to transition the technique to the field are also provided.

  9. Hopanoid-free Methylobacterium extorquens DM4 overproduces carotenoids and has widespread growth impairment

    Science.gov (United States)

    Muller, Emilie E. L.; Bringel, Françoise; Caroll, Sean M.; Pearson, Ann; Vuilleumier, Stéphane; Marx, Christopher J.

    2017-01-01

    Hopanoids are sterol-like membrane lipids widely used as geochemical proxies for bacteria. Currently, the physiological role of hopanoids is not well understood, and this represents one of the major limitations in interpreting the significance of their presence in ancient or contemporary sediments. Previous analyses of mutants lacking hopanoids in a range of bacteria have revealed a range of phenotypes under normal growth conditions, but with most having at least an increased sensitivity to toxins and osmotic stress. We employed hopanoid-free strains of Methylobacterium extorquens DM4, uncovering severe growth defects relative to the wild-type under many tested conditions, including normal growth conditions without additional stressors. Mutants overproduce carotenoids–the other major isoprenoid product of this strain–and show an altered fatty acid profile, pronounced flocculation in liquid media, and lower growth yields than for the wild-type strain. The flocculation phenotype can be mitigated by addition of cellulase to the medium, suggesting a link between the function of hopanoids and the secretion of cellulose in M. extorquens DM4. On solid media, colonies of the hopanoid-free mutant strain were smaller than wild-type, and were more sensitive to osmotic or pH stress, as well as to a variety of toxins. The results for M. extorquens DM4 are consistent with the hypothesis that hopanoids are important for membrane fluidity and lipid packing, but also indicate that the specific physiological processes that require hopanoids vary across bacterial lineages. Our work provides further support to emerging observations that the role of hopanoids in membrane robustness and barrier function may be important across lineages, possibly mediated through an interaction with lipid A in the outer membrane. PMID:28319163

  10. Site of initial diabetes education does not affect metabolic outcomes in children with T1DM.

    Science.gov (United States)

    Tonyushkina, Ksenia N; Visintainer, Paul F; Jasinski, Christopher F; Wadzinski, Thomas L; Allen, Holley F

    2014-03-01

    To determine the difference in metabolic outcomes at 1 and 2 yr post type 1 diabetes mellitus (T1DM) diagnosis in children depending on the site of initial diabetes education: inpatient, vs. outpatient, vs. mixed locations. A retrospective chart review was performed for all patients with new onset antibody positive T1DM, aged 1-18 yr old, diagnosed in 2004-2009, and followed for at least 1 yr in a diabetes program at a tertiary academic health care center. Patients were divided into three groups based on the site of initial diabetes education: inpatient, outpatient, and mixed locations. The primary outcome was A1c at 1 and 2 yr. We enrolled 238 children (133 boys), mean (± SD) age 9.9 (± 4.1). A1c levels did not differ among inpatient, outpatient, and mixed location groups at 1 and 2 yr post diagnosis (p = 0.85 and p = 0.69, respectively) and the long-acting insulin doses were similar at 1 and 2 yr (p = 0.18 and p = 0.15, respectively). There was no difference in the number of acute diabetes complications between the groups. At 1 yr, 21.8% of outpatient-educated children were on insulin pump therapy in contrast to 14.7% of inpatient and 2.7% of mixed educated groups (p = 0.04). Families of children with new onset T1DM can be successfully and safely educated in a clinic setting. An 'education' admission for a medically stable patient is not necessary most of the time, however, clinical judgment and careful assessment of the family's coping and learning capabilities are important when determining the site of education. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Telemetry System Parameters and Bit Error Performance of NRZ and DM PCM/FM

    Science.gov (United States)

    1976-03-29

    MODEL 400 4·POLE, LINEAR L ________ J PHASE FILTER ANDOM OM PSEUDO·R 0 PSEUDO·RA R NDOM :’olRZ EMR 721 TELEMETRY RECOVERED PCM SEQUENCE BIT...with variable bit rate. The NRZ and DM signals were pseudo-random sequences of 2,047 bits. The pre- modulation filter was a four-pole, linear phase ... filter with adjustable bandwidth. RF transmitter devia- tion and RF attenuation were adjustable on the FM signal generator which was operated at

  12. Geoscience Laser Altimeter System (GLAS): Final Test Report of DM LHP TV Testing. Revised

    Science.gov (United States)

    Baker, Charles

    2000-01-01

    The Demonstration Model (DM) Loop Heat Pipe (LHP) was tested at Goddard Space Flight Center (GSFC) during September and October, 1999. The LHP system was placed in the Dynavac 36 in. chamber in Building 4. The test lasted for about 6 weeks. The LHP was built, designed, and manufactured at Dynatherm Corporation, Inc. In Hunt Valley, MD according to GSFC specifications. The purpose of the test was to evaluate the performance of a propylene LHP for the Geoscience Laser Altimetry System (GLAS) instrument application.

  13. T-DM1 Combo Graduates from I-SPY 2.

    Science.gov (United States)

    2016-06-01

    The combination of the antibody-drug conjugate ado-trastuzumab emtansine, or T-DM1, plus pertuzumab given prior to surgery outperformed standard therapy in women with HER2-positive breast cancer enrolled in the I-SPY 2 trial. The results, reported at the American Association for Cancer Research Annual Meeting 2016, suggest that the drug combination would be successful in a phase III trial, potentially leading to an effective, less toxic treatment option for women at risk for metastatic disease.

  14. The Treatment of Long - duaration DM Should Be Dominated by Treating Kidney

    Institute of Scientific and Technical Information of China (English)

    FENGJizhong

    2004-01-01

    Diabetes pertains to Xiaoke in TCM. It is divided into three kinds, namely, upper lung,middle stomach and lower kidney, its treatment is also divided into moistening lung, clearing away stomach-heat and nourishing the kidney accordingly. But to get the root of the matter, the treatment of long - duration DM should be dominated by nourishing kidney and fostering the Yin. According to this theory, the Xiao Tang Yin was formulated. If consideration can be given to other symptoms and flexible modification can be made,good effects were got always.

  15. High-Contrast Coronagraph Performance in the Presence of DM Actuator Defects

    Science.gov (United States)

    Sidick, Erkin; Shaklan, Stuart; Cady, Eric

    2015-01-01

    Deformable Mirrors (DMs) are critical elements in high contrast coronagraphs, requiring precision and stability measured in picometers to enable detection of Earth-like exoplanets. Occasionally DM actuators or their associated cables or electronics fail, requiring a wavefront control algorithm to compensate for actuators that may be displaced from their neighbors by hundreds of nanometers. We have carried out experiments on our High-Contrast Imaging Testbed (HCIT) to study the impact of failed actuators in partial fulfillment of the Terrestrial Planet Finder Coronagraph optical model validation milestone. We show that the wavefront control algorithm adapts to several broken actuators and maintains dark-hole contrast in broadband light.

  16. Right-handed sneutrinos as asymmetric DM and neutrino masses from neutrinophilic Higgs bosons

    CERN Document Server

    Mitropoulos, Pantelis

    2013-01-01

    We consider an extension of the Next-to-Minimal Supersymmetric Standard Model by three right-handed neutrinos and a pair of neutrinophilic Higgs superfields. The small neutrino masses arise naturally from a small vacuum expectation value of the additional Higgs fields (hence without lepton number violation), while the lightest right-handed sneutrinos can constitute asymmetric Dark Matter. The right-handed sneutrino and baryon asymmetries are connected through equilibrium processes in the early universe, explaining the coincidence of the DM and baryon abundances. We show that particle physics and astrophysical constraints are satisfied.

  17. Localized spin excitations in an antiferromagnetic spin system with D-M interaction

    Energy Technology Data Exchange (ETDEWEB)

    Evangeline Rebecca, T.; Latha, M. M., E-mail: lathaisaac@yahoo.com [Department of Physics, Women' s Christian College, Nagercoil 629 001 (India)

    2016-06-15

    The existence of localized spin excitations and spin deviations along the site in a one-dimensional antiferromagnet with Dzyaloshinski-Moriya (D-M) interaction has been studied using quasiclassical approximation. By introducing the Holstein-Primakoff bosonic representation of spin operators, the coherent state ansatz, and the time dependent variational principle, a discrete set of coupled nonlinear partial differential equations governing the dynamics is derived. Employing the multiple-scale method, one, two and three solitary wave solutions are constructed and depicted graphically.

  18. Implementation of area optimization precoder in a 40 Gb/s PolDM-DQPSK system

    Institute of Scientific and Technical Information of China (English)

    ZHOU Li-ming; ZHANG Yang-an; ZHANG Ming-lun; WANG Gai; ZHANG Jin-nan; HUANG Yong-qing; LI Ling

    2010-01-01

    @@ In this paper,a new model based on an improved Brent Kung(BK)parallel prefix network(PPN)algorithm is proposed and realized in the field programmable gate array(FPGA).This model is employed in the implementation of 20 Gb/s differential quadrature phase-shift keying(DQPSK)precoder in 40 Gb/s polarization division multiplex(PolDM)DQPSK system.In the computation process,the computation complexity(area)optimization with fan-out limited is achieved.In the implementation,770 FPGA slice registers are utilized,which save about 60% logic resources compared with the previous Kogge Stone(KS)algorithm.

  19. Advanced Adaptive Optics Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S

    2001-09-18

    The NSF Center for Adaptive Optics (CfAO) is supporting research on advanced adaptive optics technologies. CfAO research activities include development and characterization of micro-electro-mechanical systems (MEMS) deformable mirror (DM) technology, as well as development and characterization of high-resolution adaptive optics systems using liquid crystal (LC) spatial light modulator (SLM) technology. This paper presents an overview of the CfAO advanced adaptive optics technology development activities including current status and future plans.

  20. A rapid polymerase chain reaction-based test for screening Steinert′s disease (DM1

    Directory of Open Access Journals (Sweden)

    Hamzi Khalil

    2010-01-01

    Full Text Available Myotonic dystrophy (DM is a multisystemic neuromuscular disorder caused by a dynamic mutation of (CTG trinucleotide repeats in the 3′ untranslated region of the myotonic dystrophy protein kinase gene (DMPK. The aim of the present study was to establish the use of polymerase chain reaction (PCR-based simple and rapid method for initial sample screening. Only a minority of samples were tested positive with the above method and need to be detected by tri primer (TP-PCR and Southern blotting which is more time consuming and involves use of radioactive material. This study concerned 24 patients from nine families with a clinical diagnosis of the DM1. DNA extracted from the blood was used for amplification of the triplet repeat sequences at the DMPK loci. We obtained two bands for the normal subjects and one band for patients corresponding to normal DMPK allele, confirmed by the TP-PCR and the Southern blot. This rapid test for initial screening of samples for the presence of DMPK mutations is economical and reliable method. This method reduces the number of samples needing TP-PCR and Southern blotting.

  1. TREX-DM: a low background Micromegas-based TPC for low mass WIMP detection

    CERN Document Server

    Iguaz, F J; Aznar, F; Castel, J F; Cebrian, S; Dafni, T; Garcia, J A; Gomez, H; Gonzalez-Diaz, D; Irastorza, I G; Lagraba, A; Luzon, G; Peiro, A; Rodriguez, A

    2015-01-01

    Dark Matter experiments are recently focusing their detection techniques in low-mass WIMPs, which requires the use of light elements and low energy threshold. In this context, we present the TREX-DM experiment, a low background Micromegas-based TPC for low-mass WIMP detection. Its main goal is the operation of an active detection mass $\\sim$0.300 kg, with an energy threshold below 0.4 keVee and fully built with previously selected radiopure materials. This article describes the actual setup, the first results of the comissioning in Ar+2\\%iC$_4$H$_{10}$ at 1.2 bar and the future updates for a possible physics run at the Canfranc Underground Laboratory in 2016. A first background model is also presented, based on Geant4 simulations and a muon/electron discrimination method. In a conservative scenario, TREX-DM could be sensitive to DAMA/LIBRA and other hints of positive WIMPs signals, with some space for improvement with a neutron/electron discrimination method or the use of other light gases.

  2. PPPC 4 DM ID: a poor particle physicist cookbook for dark matter indirect detection

    Energy Technology Data Exchange (ETDEWEB)

    Cirelli, Marco; Panci, Paolo; Strumia, Alessandro [CERN Theory Division, CH-1211 Geneve (Switzerland); Corcella, Gennaro [Museo Storico della Fisica, Centro Studi e Ricerche E. Fermi, P. del Viminale 1, I-00185 Rome (Italy); Hektor, Andi; Kadastik, Mario; Raidal, Martti [National Institute of Chemical Physics and Biophysics, Ravala 10, 10143 Tallinn (Estonia); Hütsi, Gert [Tartu Observatory, Tõravere 61602 (Estonia); Sala, Filippo, E-mail: marco.cirelli@cea.fr [Scuola Normale Superiore, Piazza dei Cavalieri 7, I-56126 Pisa (Italy)

    2011-03-01

    We provide ingredients and recipes for computing signals of TeV-scale Dark Matter annihilations and decays in the Galaxy and beyond. For each DM channel, we present the energy spectra at production, computed by high-statistics simulations. We estimate the Monte Carlo uncertainty by comparing the results yielded by the Pythia and Herwig event generators. We then provide the propagation functions for charged particles in the Galaxy, for several DM distribution profiles and sets of propagation parameters. Propagation of e{sup ±} is performed with an improved semi-analytic method that takes into account position-dependent energy losses in the Milky Way. Using such propagation functions, we compute the energy spectra of e{sup ±}, p-bar and d-bar at the location of the Earth. We then present the gamma ray fluxes, both from prompt emission and from Inverse Compton scattering in the galactic halo. Finally, we provide the spectra of extragalactic gamma rays. All results are available in numerical form and ready to be consumed.

  3. Micromegas for dark matter searches: CAST/IAXO & TREX-DM

    CERN Document Server

    Garza, J G; Castel, J.F.; Cebrián, S.; Dafni, T.; Ferrer-Ribas, E.; Galán, J.; García, J.A.; Giomataris, I.; Iguaz, F.J.; Irastorza, I.G.; Luzón, G.; Mirallas, H.; Papaevangelou, T.; Peiró, A.; Tomás, A.; Vafeiadis, T.

    2016-01-01

    The most compelling candidates for Dark Matter to day are WIMPs and axions. The applicability of gasesous Time Projection Chambers (TPCs) with Micromesh Gas Structures (Micromegas) to the search of these particles is explored within this work. Both particles would produce an extremely low rate at very low energies in particle detectors. Micromegas detectors can provide both low background rates and low en- ergy threshold, due to the high granularity, radiopurity and uniformity of the readout. Small (few cm wide) Micromegas detectors are used to image the axion-induced x-ray signal expected in the CERN Axion Solar Telescope (CAST) experiment. We show the background levels obtained in CAST and the prospects to further reduce them to the values required by the Internation Axion Observatory (IAXO). We also present TREX-DM, a scaled-up version of the Micromegas used in axion research, but this time dedicated to the low-mass WIMP detection. TREX-DM is a high-pressure Micromegas-based TPC designed to host a few hund...

  4. Chromospheric activity on late-type star DM UMa using high-resolution spectroscopic observations

    CERN Document Server

    Zhang, LiYun; Han, Xianming L; Chang, Liang; Wang, Daimei

    2016-01-01

    We present new 14 high-resolution echelle spectra to discuss the level of chromospheric activity of DM UMa in {He I} D3, {Na I} D1, D2, Halpha, and {Ca II} infrared triplet lines (IRT). It is the first time to discover the emissions above the continuum in the {He I} D3 lines on 2015 February 9 and 10. The emission on February 9 is the strongest one ever detected for DM UMa. We analysed these chromospheric active indicators by employing the spectral subtraction technique. The subtracted spectra reveal weak emissions in the {Na I} D1, D2 lines, strong emission in the Halpha line, and clear excess emissions in the {Ca II} IRT lines. Our values for the EW8542/EW8498 ratio are on the low side, in the range of 1.0-1.7. There are also clear phase variations of the level of chromospheric activity in equivalent width (EW) light curves in these chromospheric active lines (especially the Halpha line). These phenomena might be explained by flare events or rotational modulations of the level of chromospheric activity.

  5. SOLA-DM: A numerical solution algorithm for transient three-dimensional flows

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, T.L.; Nichols, B.D.; Hirt, C.W.; Stein, L.R.

    1988-02-01

    SOLA-DM is a three-dimensional time-explicit, finite-difference, Eulerian, fluid-dynamics computer code for solving the time-dependent incompressible Navier-Stokes equations. The solution algorithm (SOLA) evolved from the marker-and-cell (MAC) method, and the code is highly vectorized for efficient performance on a Cray computer. The computational domain is discretized by a mesh of parallelepiped cells in either cartesian or cylindrical geometry. The primary hydrodynamic variables for approximating the solution of the momentum equations are cell-face-centered velocity components and cell-centered pressures. Spatial accuracy is selected by the user to be first or second order; the time differencing is first-order accurate. The incompressibility condition results in an elliptic equation for pressure that is solved by a conjugate gradient method. Boundary conditions of five general types may be chosen: free-slip, no-slip, continuative, periodic, and specified pressure. In addition, internal mesh specifications to model obstacles and walls are provided. SOLA-DM also solves the equations for discrete particle dynamics, permitting the transport of marker particles or other solid particles through the fluid to be modeled. 7 refs., 7 figs.

  6. Data Clustering Menggunakan Metodologi CRISP-DM Untuk Pengenalan Pola Proporsi Pelaksanaan Tridharma

    Directory of Open Access Journals (Sweden)

    Irwan Budiman

    2014-01-01

    Full Text Available Quality of human resources faculty can be reflected from the implementation of productivity and quality Tridharma (education, research, community service  and  supporting  field  activities.  Lecturer  Workload  and Evaluation of  Higher Education  Tridharma  (BKD  and theEPT-PT  aims  to  ensure  the  implementation  of  the  faculty  task  runs  according  to  the  criteria  set  out  in  legislation.  Data  clusteringTridharma  implementation is needed to  get  some  knowledge  of the  pattern of Tridharma  implementation  at  college.  Clustering  as a  data mining  technique  should be  scalable, reliable  and  meet  an  agreed  standard.  CRISP-DM is the standardization of  data mining  is  used  in this study. The results of data clustering found the pattern of proportion of Tridharma  into 3 clusters representing patterns: professionals, managers and teachers.Keywords : Clustering, CRISP-DM, K-Means, Tridharma

  7. DM/DE revealed: The physical origin of Lambda-CDM

    Science.gov (United States)

    Beichler, James

    2009-10-01

    In the past few decades two new `crises' affecting fundamental physics have emerged in the observation of phenomena that indicate the existence of Dark Matter and Dark Energy. These are not problems which can be solved by quantum theory or particles, but rather problems that are related to gravity theory as expressed by the general theory of relativity. Numerous ideas and hypotheses have been suggested to explain these new problematic phenomena, but no particular hypothesis or resulting model has proven satisfactory. No model yet proposed seems to be able to explain either DM and DE or both even though theoretical physicists generally agree that the two should have a single common explanation. There is, however, one relatively unknown exception that can explain both DM and DE without any additional hypotheses and this model explains the physical origins of the Lambda-CDM term that others have already proposed adding to general relativity. Yet this new model is not without consequences for the rest of physics and science: Accepting this new model would mean acknowledging the existence of a higher space-like dimension in addition to our normal four-dimensions of space-time.

  8. An estimate of the DM profile in the Galactic bulge region

    CERN Document Server

    Iocco, Fabio

    2016-01-01

    We present an analysis of the mass distribution in the region of the Galactic bulge, which leads to constraints on the total amount and distribution of Dark Matter (DM) therein. Our results -based on the dynamical measurement of the BRAVA collaboration- are quantitatively compatible with those of a recent analysis, and generalised to a vaste sample of observationally inferred morphologies of the stellar components in the region of the Galactic bulge. By fitting the inferred DM mass to a generalised NFW profile, we find that cores (index gamma smaller than 0.6) are forbidden only for very light configurations of the bulge, and that cusps (index gamma bigger than 1.2) are allowed, but not necessarily preferred. Interestingly, we find that the results for the bulge region are compatible with those obtained with dynamical methods (based on the rotation curve) applied to outer regions of the Milky Way, for all morphologies adopted. We find that the uncertainty on the shape of the stellar morphology heavily affects...

  9. Identification of an extracellular antifungal protein from the endophytic fungus Colletotrichum sp. DM06.

    Science.gov (United States)

    Dey, Prabuddha; Kamdar, Maulik R; Mandal, Santi M; Maiti, Mrinal K

    2013-02-01

    An extracellular antifungal protein of 28 kDa (exAFP-C28) was identified from an endophytic fungus Colletotrichum sp. DM-06. After purification, the MIC value of exAFP-C28 against Candida albicans, a well-known human pathogenic fungus was found to be 32 μg/mL that unaffected the human red blood cells. The antifungal activity associated with exAFP-C28 was manifested by the increased membrane permeability of C. albicans cells followed by disruption. Proteomics and bioinformatics analyses revealed that several peptide fragments of exAFP-C28 have identity with the bacterial 50S ribosomal protein L10, and a stretch of 55 amino acids of two peptide fragments corresponding to the Nterminus of L10 protein is capable of forming amphipathic helix required for membrane penetration. Taken together, our results suggest that the exAFP-C28 protein from Colletotrichum sp. DM-06 is a promising therapeutic agent in controlling candidiasis disease in animals including humans.

  10. Measurement of the attenuation length of argon scintillation light in the ArDM LAr TPC

    CERN Document Server

    Calvo, J; Crivelli, P; Daniel, M; DiLuise, S; Gendotti, A; Horikawa, S; Molina-Bueno, L; Montes, B; Mu, W; Murphy, S; Natterer, G; Ngyuen, K; Periale, L; Quan, Y; Radics, B; Regenfus, C; Romero, L; Rubbia, A; Santorelli, R; Sergiampietri, F; Viant, T; Wu, S

    2016-01-01

    We report on a measurement of the attenuation length for the scintillation light in the tonne size liquid argon target of the ArDM dark matter experiment. The data was recorded in the first underground operation of the experiment in single-phase operational mode. The results were achieved by comparing the light yield spectra from 39-Ar and 83m-Kr to a description of the ArDM setup with a model of full light ray tracing. A relatively low value close to 0.5 m was found for the attenuation length of the liquid argon bulk to its own scintillation light. We interpret this result as a presence of optically active impurities in the liquid argon which are not filtered by the installed purification systems. We also present analyses of the argon gas employed for the filling and discuss cross sections in the vacuum ultraviolet of various molecules in respect to purity requirements in the context of large liquid argon installations.

  11. CMIN — a CRISP-DM-based case tool for supporting data mining projects

    Directory of Open Access Journals (Sweden)

    Carlos Cobos

    2010-10-01

    Full Text Available This paper introduces CMIN, an integrated computer aided software engineering (CASE tool based on cross-industry standard process for data mining (CRISP-DM 1.0 designed to support carrying out data mining projects. It is “integrated” in the sense that it supports all phases of a process. A general overview of how CMIN works is presented first, including a treatment of processes, templates and project management. CMIN’s capacity for easily and intuitively monitoring projects is highlighted, as is the manner in which CMIN allows a user to increase knowledge regarding using CRISP-DM or any other process defined in the CASE tool through the help and information presented in each step. Next, it is shown how CMIN can bind new data mining algorithms in runtime (without the need to recompile the tool to support modelling tasks (based on a Workflow and evaluate data mining projects. Finally, the results of two evaluations of the tool, some conclusions and suggestions for future work are presented.

  12. Non-diabetic renal disease (NDRD) in patients with type 2 diabetes mellitus (type 2 DM).

    Science.gov (United States)

    Prakash, Jai

    2013-03-01

    In contrast to Type 1 diabetes mellitus (DM), the incidence of non-diabetic renal disease (NDRD) is very high in Type 2 diabetic patients. A wide spectrum of non-diabetic nephropathy (NDN) including both glomerular and tubulointerstitial lesions are reported in patients with Type 2 DM and their precise diagnosis requires histological examination of kidney tissue. Renal biopsy studies suggest that 25-50% of patients with Type 2 diabetes had glomerular lesions unrelated to or in addition to diabetic nephropathy. Histological studies confirm that NDRD can occur in isolated form without diabetic nephropathy or superimposed on diabetic nephropathy. Diabetic nephropathy can occur in absence of retinopathy and chance of getting diabetic and non-diabetic renal lesions are nearly equal in Type 2 diabetic patient in absence of diabetic retinopathy (RP). The presence of RP suggests the concurrence of DN, but does not exclude non-diabetic nephropathy. Clearly, renal biopsy is indicated in proteinuric Type 2 diabetic patients for precise diagnosis of diabetic vs non-diabetic renal disease. Appropriate treatment of NDRD is associated with good clinical outcome. Thus, it is gratifying to treat NDRD in selected patients. Besides, 40 to 60% of ESRD in Type 2 diabetic patients is not caused by diabetic nephropathy.

  13. Efek perlindungan kombinasi kuersetin dan omega-3 terhadap sel β pankreas tikus dm tipe 2

    Directory of Open Access Journals (Sweden)

    Asri Hendrawati

    2017-06-01

    Full Text Available Diabetes mellitus (DM is a metabolic disease that causes damage pancreatic cells. Quercetin and omega-3 widely studied as an adjunctive therapy in DM because it can reduce oxidative stress and prevent cell damage. The aim of the study was to measure the ability of quercetin and omega-3 combination in protecting pancreatic β-cells in diabetic rats. Subjects consisted of 28 rats, divided into 1 healthy group and 6 diabetic groups (4 rats per group. The tested materials are quercetin doses 5, 20 and 80 mg/kg/day and omega-3 doses 25, 100 and 400mg/kg /day orally for 4 weeks. After treatment, the pancreatic tissue was taken for hematoxylin-eosin staining and be calculated the percentage of it damage. In this research, combination of quercetin 80mg/kg/day and omega-3 400 mg /kg/day lowered levels of destructionof pancreatic β cells significantly better than a smaller dose combination or without the combination (p<0,05. So, combination of quercetin 80 mg/kg/day and omega-3 400 mg/kg/day lowered levels of destruction of pancreatic β-cells most significantly better than a smaller ose combination or without the combination.

  14. Evolution of protoplanetary disks: Constraints from DM Tauri and GM Aurigae

    CERN Document Server

    Hueso, R; Hueso, Ricardo; Guillot, Tristan

    2005-01-01

    We present a one-dimensional model of the formation and viscous evolution of protoplanetary disks. The formation of the early disk is modeled as the result of the gravitational collapse of an isothermal molecular cloud. The disk's viscous evolution is integrated according to two parameterizations of turbulence: The classical $\\alpha$ representation and a $\\beta$ parameterization, representative of non-linear turbulence driven by the keplerian shear. We apply the model to DM Tau and GM Aur, two classical T-Tauri stars with relatively well-characterized disks, retrieving the evolution of their surface density with time. We perform a systematic Monte-Carlo exploration of the parameter space (i.e. values of the $\\alpha$-$\\beta$ parameters, and of the temperature and rotation rate in the molecular cloud) to find the values that are compatible with the observed disk surface density distribution, star and disk mass, age and present accretion rate. We find that the observations for DM Tau require $0.001<\\alpha<...

  15. [Functional activity of the modA, gene in Methylobacterium dichloromethanicum DM4].

    Science.gov (United States)

    Firsova, Y E; Trotsenko, Y A

    2014-01-01

    The putative METDI2644 (modA2) gene of Methylobacterium dichloromethanicum DM4, present in the 126-kbp chromosomal fragment associated with dichloromethane (DCM) degradation was investigated. While this gene is presumed to encode the periplasmic substrate-binding subunit of the molybdate ABC transporter, its conceptual translation also exhibits similarity to the proteins containing the ostA conservative domain and responsible for resistance of gram-negative bacteria to organic solvents. Reverse transcription polymerase chain reaction (RT-PCR) revealed the RNA transcripts of this gene in the cells grown on either DCM or methanol. The mobilizable suicide vector pK18mob was used to obtain a knockout mutant with the METDI2644 gene inactivated by insertion of the gentamycin cassette. The mutant pregrown on methanol exhibited lower growth rate on DCM than the wild-type strain DM4. The difference was not alleviated by addition of sodium molybdate. Our results suggest that the METDI2644 gene product plays a role in cell adaptation to DCM degradation.

  16. PPARs in obesity-induced T2DM, dyslipidaemia and NAFLD.

    Science.gov (United States)

    Gross, Barbara; Pawlak, Michal; Lefebvre, Philippe; Staels, Bart

    2017-01-01

    Obesity is a worldwide epidemic that predisposes individuals to cardiometabolic complications, such as type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), which are all related to inappropriate ectopic lipid deposition. Identification of the pathogenic molecular mechanisms and effective therapeutic approaches are highly needed. The peroxisome proliferator-activated receptors (PPARs) modulate several biological processes that are perturbed in obesity, including inflammation, lipid and glucose metabolism and overall energy homeostasis. Here, we review how PPARs regulate the functions of adipose tissues, such as adipogenesis, lipid storage and adaptive thermogenesis, under healthy and pathological conditions. We also discuss the clinical use and mechanism of PPAR agonists in the treatment of obesity comorbidities such as dyslipidaemia, T2DM and NAFLD. First generation PPAR agonists, primarily those acting on PPARγ, are associated with adverse effects that outweigh their clinical benefits, which led to the discontinuation of their development. An improved understanding of the physiological roles of PPARs might, therefore, enable the development of safe, new PPAR agonists with improved therapeutic potential.

  17. SY 10-2 THE ROLE OF SGLT2 INHIBITORS IN THE MANAGEMENT OF T2DM.

    Science.gov (United States)

    Jung, Chang Hee

    2016-09-01

    Type 2 diabetes mellitus (T2DM) is a complex endocrine and metabolic disorder and is a major global public health problem with a rapidly increasing prevalence. Although a wide range of pharmacotherapy for glycemic control in T2DM is now available, management of T2DM remains complex and challenging. The kidneys contribute to glucose homeostasis primarily by the glucose reabsorption from the glomerular filtrate. The sodium-glucose co-transporter 2 (SGLT2) inhibitors, a new class of antidiabetes agents that inhibit glucose absorption from the kidney tubule independent of insulin, offer a unique opportunity to improve outcomes for patient with T2DM.In this session, I'd like to discuss several topics regarding SGLT2 inhibitors including their glucose lowering effects, recently issued adverse effects and long-term cardiovascular benefits from the perspectives of endocrinologist.

  18. Biohydrogen production from desugared molasses (DM) using thermophilic mixed cultures immobilized on heat treated anaerobic sludge granules

    DEFF Research Database (Denmark)

    Kongjan, Prawit; O-Thong, Sompong; Angelidaki, Irini

    2011-01-01

    Hydrogen production from desugared molasses (DM) was investigated in both batch and continuous reactors using thermophilic mixed cultures enriched from digested manure by load shock (loading with DM concentration of 50.1 g-sugar/L) to suppress methanogens. H2 gas, free of methane, was produced...... by Thermoanaerobacterium spp., which are key players in fermentative hydrogen production of DM under thermophilic conditions. Furthermore, the granules in the UASB reactor were also significantly containing Thermoanaerobacterium spp. and phylum Firmecutes (most Clotridium, Bacillus and Desulfobacterium....... The enriched hydrogen producing mixed culture achieved from the 16.7 g-sugars/L DM batch cultivation was immobilized on heat treated anaerobic sludge granules in an up-flow anaerobic sludge blanket (UASB) reactor. The UASB reactor, operated at a hydraulic retention time (HRT) of 24 h fed with 16.7 g...

  19. The interaction of the antitoxin DM43 with a snake venom metalloproteinase analyzed by mass spectrometry and surface plasmon resonance

    DEFF Research Database (Denmark)

    Brand, Guilherme D; Salbo, Rune; Jørgensen, Thomas J D

    2012-01-01

    obtained after successive injections of DM43 in a concentration series were globally fitted to a simple bimolecular interaction, yielding the following kinetic rates for the DM43/jararhagin interaction: k(a) = 3.54 ± 0.03 × 10(4) M(-1) s(-1) and k(d) = 1.16 ± 0.07 × 10(-5) s(-1), resulting...

  20. Influence of ring size on the cognition-enhancing activity of DM235 and MN19, two potent nootropic drugs.

    Science.gov (United States)

    Guandalini, L; Martini, E; Di Cesare Mannelli, L; Dei, S; Manetti, D; Scapecchi, S; Teodori, E; Ghelardini, C; Romanelli, M N

    2012-03-01

    A series of analogs of DM235 and MN19, characterized by rings with different size, have been prepared and evaluated for their nootropic activity in the mouse passive-avoidance test. It was found that the optimal ring size for the analogs of DM235, showing endocyclic both amidic groups, is 6 or 7 atoms. For the compounds structurally related to MN19, carrying an exocyclic amide group, the piperidine ring is the moiety which gives the most interesting compounds.

  1. Substituted piperazines as nootropic agents: 2- or 3-phenyl derivatives structurally related to the cognition-enhancer DM235.

    Science.gov (United States)

    Guandalini, Luca; Martino, Maria Vittoria; Di Cesare Mannelli, Lorenzo; Bartolucci, Gianluca; Melani, Fabrizio; Malik, Ruchi; Dei, Silvia; Floriddia, Elisa; Manetti, Dina; Orlandi, Francesca; Teodori, Elisabetta; Ghelardini, Carla; Romanelli, Maria Novella

    2015-04-15

    A series of 2-phenyl- or 3-phenyl piperazines, structurally related to DM235 and DM232, two potent nootropic agents, have been prepared and tested in the mouse passive-avoidance test, to assess their ability to revert scopolamine-induced amnesia. Although the newly synthesized molecules were less potent than the parent compounds, some useful information has been obtained from structure-activity relationships. A small but significant enantioselectivity has been found for the most potent compound 5a.

  2. History of Cesarean Section Associated with Childhood Onset of T1DM in Newfoundland and Labrador, Canada

    Directory of Open Access Journals (Sweden)

    J. Phillips

    2012-01-01

    Full Text Available Objectives. Newfoundland and Labrador (NL has one of the highest incidences of Type 1 diabetes mellitus (T1DM worldwide. Rates of T1DM are increasing and the search for environmental factors that may be contributing to this increase is continuing. Methods. This was a population-based case control design involving the linkage of data from a diabetes database with live birth registration data. 266 children aged 0–15 years with T1DM were compared to age- and gender-matched controls. Chi-square analysis and multivariate conditional logistic regression were carried out to assess maternal and infant factors (including maternal age, marital status, education, T1DM, hypertension, birth order, delivery method, gestational age, size-for-gestational-age, and birth weight. Results. Cases of T1DM were more likely to be large-for-gestational-age (P=0.024 and delivered by C-section (P=0.009 as compared to controls. C-section delivery was associated with increased risk of T1DM (HR 1.41, P=0.015 when birth weight and gestational age were included in the model, but not when size-for-gestational-age was included (HR 1.3, P=0.076. Conclusions. Birth by C-section was found to be a risk factor for the development of T1DM in a region with high rates of T1DM and birth by C-section. These findings may have an impact on health practice, health care planning, and future research.

  3. Hubungan Tingkat Keteraturan Berolahraga Terhadap Komplikasi Penyakit pada Pasien DM Tipe 2 di Poliklinik Endokrin RSUP DR. M. Djamil Padang

    Directory of Open Access Journals (Sweden)

    Reza Ekatama Rajasa

    2016-08-01

    Full Text Available AbstrakAda banyak faktor yang mempengaruhi komplikasi pada pasien DM tipe 2, salah satunya adalah keteraturan berolahraga. Aktivitas fisik yang kurang akan  berisiko terjadinya hiperglikemia. Kondisi ini lambat laun akan menyebabkan kerusakan mikrovaskular dan makrovaskular. Olahraga yang teratur dapat membuat normal gula darah. Tujuan penelitian ini adalah meneliti lebih lanjut tentang hubungan keteraturan berolahraga terhadap komplikasi DM tipe 2. Penelitian ini dilakukan pada masyarakat yang berkunjung ke poliklinik RSUP Dr. M. Djamil Padang. Ini merupakan studi retrospektif dengan jumlah subjek 73 orang. Pengumpulan data responden dilakukan dengan wawancara dan rekam medis. Analisis statistik yang digunakan adalah uji chi-square.Hasil penelitian ini menunjukkan bahwa 65,8% responden menderita 3 atau lebih komplikasi (banyak, sedangkan 34,2% menderita kurang dari 3 komplikasi (sedikit. Kesimpulan studi ini adalah terdapat hubungan antara keteraturan berolahraga dengan komplikasi DM tipe 2.Kata kunci: keteraturan berolahraga, komplikasi DM tipe 2AbstractThere are many factors correlate to complications in  type 2 diabetes mellitus (DM patients, one of them is exercise regularity. Lack of physical activity can lead to the hiperglycemia condition. It  could be the cause of  micovascular and macrovascular injuries. Exercise regularity can lead blood glucose toward normal. The objective of this study was to investigate the correlation of exercise regularity to the type 2 DM complications.The research was conducted to the  people who visit polyclynic of M. Djamil Hospital. It is a retrospective study with 73 subjek. Data collection was performed through interviews and using medical records. The statistical analysis was performed chi-square test.The result of this research found that 65.8% of respondent had many complication ( three or more, whereas 34.2% of had less than three complications. Analysis by chi square test, concluded that there

  4. A novel method to measure HLA-DM-susceptibility of peptides bound to MHC class II molecules based on peptide binding competition assay and differential IC(50) determination.

    Science.gov (United States)

    Yin, Liusong; Stern, Lawrence J

    2014-04-01

    HLA-DM (DM) functions as a peptide editor that mediates the exchange of peptides loaded onto MHCII molecules by accelerating peptide dissociation and association kinetics. The relative DM-susceptibility of peptides bound to MHCII molecules correlates with antigen presentation and immunodominance hierarchy, and measurement of DM-susceptibility has been a key effort in this field. Current assays of DM-susceptibility, based on differential peptide dissociation rates measured for individually labeled peptides over a long time base, are difficult and cumbersome. Here, we present a novel method to measure DM-susceptibility based on peptide binding competition assays performed in the presence and absence of DM, reported as a delta-IC(50) (change in 50% inhibition concentration) value. We simulated binding competition reactions of peptides with various intrinsic and DM-catalyzed kinetic parameters and found that under a wide range of conditions the delta-IC(50) value is highly correlated with DM-susceptibility as measured in off-rate assay. We confirmed experimentally that DM-susceptibility measured by delta-IC(50) is comparable to that measured by traditional off-rate assay for peptides with known DM-susceptibility hierarchy. The major advantage of this method is that it allows simple, fast and high throughput measurement of DM-susceptibility for a large set of unlabeled peptides in studies of the mechanism of DM action and for identification of CD4+ T cell epitopes.

  5. CTG repeats distribution and Alu insertion polymorphism at myotonic dystrophy (DM) gene in Amhara and Oromo populations of Ethiopia.

    Science.gov (United States)

    Gennarelli, M; Pavoni, M; Cruciani, F; De Stefano, G; Dallapiccola, B; Novelli, G

    1999-01-01

    Myotonic dystrophy (DM) is a dominantly inherited neuromuscular disease, highly variable and multisystemic, which is caused by the expansion of a CTG repeat located in the 3' untranslated region of the DMPK gene. Normal alleles show a copy number of 5-37 repeats on normal chromosomes, amplified to 50-3000 copies on DM chromosomes. The trinucleotide repeat shows a trimodal allele distribution in the majority of the examined population. The first class includes alleles carrying (CTG)5, the second class, alleles in the range 7-18 repeats, and the third class, alleles (CTG) > or =19. The frequency of this third class is directly related to the prevalence of DM in different populations, suggesting that normal large-sized alleles predispose toward DM. We studied CTG repeat allele distribution and Alu insertion and/or deletion polymorphism at the myotonic dystrophy locus in two major Ethiopian populations, the Amhara and Oromo. CTG allele distribution and haplotype analysis on a total of 224 normal chromosomes showed significant differences between the two ethnic groups. These differences have a bearing on the out-of-Africa hypothesis for the origin of the DM mutation. In addition, (CTG) > or =19 were exclusively detected in the Amhara population, confirming the predisposing role of these alleles compared with the DM expansion-mutation.

  6. Development of a genomic DNA reference material panel for myotonic dystrophy type 1 (DM1) genetic testing.

    Science.gov (United States)

    Kalman, Lisa; Tarleton, Jack; Hitch, Monica; Hegde, Madhuri; Hjelm, Nick; Berry-Kravis, Elizabeth; Zhou, Lili; Hilbert, James E; Luebbe, Elizabeth A; Moxley, Richard T; Toji, Lorraine

    2013-07-01

    Myotonic dystrophy type 1 (DM1) is caused by expansion of a CTG triplet repeat in the 3' untranslated region of the DMPK gene that encodes a serine-threonine kinase. Patients with larger repeats tend to have a more severe phenotype. Clinical laboratories require reference and quality control materials for DM1 diagnostic and carrier genetic testing. Well-characterized reference materials are not available. To address this need, the Centers for Disease Control and Prevention-based Genetic Testing Reference Material Coordination Program, in collaboration with members of the genetic testing community, the National Registry of Myotonic Dystrophy and Facioscapulohumeral Muscular Dystrophy Patients and Family Members, and the Coriell Cell Repositories, has established and characterized cell lines from patients with DM1 to create a reference material panel. The CTG repeats in genomic DNA samples from 10 DM1 cell lines were characterized in three clinical genetic testing laboratories using PCR and Southern blot analysis. DMPK alleles in the samples cover four of five DM1 clinical categories: normal (5 to 34 repeats), mild (50 to 100 repeats), classical (101 to 1000 repeats), and congenital (>1000 repeats). We did not identify or establish Coriell cell lines in the premutation range (35 to 49 repeats). These samples are publicly available for quality control, proficiency testing, test development, and research and should help improve the accuracy of DM1 testing.

  7. Characterization of a noncytotoxic bacteriocin from probiotic Lactobacillus plantarum DM5 with potential as a food preservative.

    Science.gov (United States)

    Das, Deeplina; Goyal, Arun

    2014-10-01

    The aim of this work was to purify and characterize the bacteriocin produced by probiotic Lactobacillus plantarum DM5 in order to evaluate its potential as nutraceuticals. Lb. plantarum DM5 exhibited in vitro probiotic properties such as high resistance to gastric juice and bile salt, adherence to human adenocarcinoma (HT-29) cells, bile salt hydrolase and cholesterol assimilation activity. Moreover, Lb. plantarum DM5 showed bacteriocin activity against several major food borne pathogens. Zymogram analysis of purified bacteriocin (plantaricin DM5) showed a molecular size of ∼15.2 kDa. Plantaricin DM5 was sensitive to proteolytic enzymes but stable in the pH range of 2.0-10.0, and it was heat resistant (121 °C for 15 min) and remained active upon treatment with surfactants and detergents. Cytotoxicity analysis of plantaricin DM5 on human embryonic kidney 293 (HEK 293) and human cervical cancer (HeLa) cell lines revealed its nontoxic and biocompatible nature. To the best of our knowledge, this is the first study on the isolated strain expressing probiotic properties and broad antimicrobial activity without any cytotoxic effect on mammalian cells from indigenous fermented beverage Marcha from India, and thus contributes to the food industry as a novel bio-preservant.

  8. Inhibition of HLA-DM mediated MHC class II peptide loading by HLA-DO promotes self tolerance

    Directory of Open Access Journals (Sweden)

    Lisa K. Denzin

    2013-12-01

    Full Text Available Major histocompatibility class II (MHCII molecules are loaded with peptides derived from foreign and self-proteins within the endosomes and lysosomes of antigen presenting cells (APCs. This process is mediated by interaction of MHCII with the conserved, nonpolymorphic MHCII-like molecule HLA-DM (DM. DM activity is directly opposed by HLA-DO (DO, another conserved, non-polymorphic MHCII like molecule. DO is an MHCII substrate mimic. Binding of DO to DM prevents MHCII from binding to DM, thereby inhibiting peptide loading. Inhibition of DM function enables low stability MHC complexes to survive and populate the surface of APCS. As a consequence, DO promotes the display of a broader pool of low abundance self-peptides. Broadening the peptide repertoire theoretically reduces the likelihood of inadvertently acquiring a density of self-ligands that is sufficient to activate self-reactive T cells. One function of DO, therefore, is to promote T cell tolerance by shaping the visible image of self. Recent data also shows that DO influences the adaptive immune response by controlling B cell entry into the germinal center reaction. This review explores the data supporting these concepts.

  9. Bioinformatics Analysis of Mitochondrial Transcription Termination Factor DmTTF Protein in Drosophila melanogaster%黑腹果蝇线粒体转录终止因子DmTTF蛋白的生物信息学分析

    Institute of Scientific and Technical Information of China (English)

    熊伟; 张晓娟; 左绍远; 张海洋; 冯慕华

    2015-01-01

    Objective: To analyze the structure and function of mitochondrial transcription termination factor DmTTF protein in Drosophila melanogaster based on bioinformatics method. Methods:Based on the amino acid sequence of mitochondrial transcription termination factor DmTTF protein in D. melanogaster from FlyBase database, the protein physicochemical property, hydrophilicity/hydrophobicity, subcellular localization, secondary protein structure, protein domain, protein tertiary structure, protein-protein interaction, as well as the protein evolution relationship of different species were analyzed by using different bioinformatics software. Results: The mitochondrial transcription termination factor DmTTF protein consists of 410 amino acids, the estimated theoretical molecular weight and is oelectric point were 48.3 kDa and 9.62 respectively, which belong to the MTERF protein superfamily without transmembrane region, and it is a hydrophilic protein. The main composition of the DmTTF protein secondary structure were α-helix and random coil, showed similar result with protein tertiary structure whose spatial structure and the coverage of template protein 3mvb.1.A was 74.0%. Proteins that interact with DmTTF were mitochondrial transcription factors or mitochondrial respiratory chain proteins. DmTTF protein in D. melanogaster and encoded homologous protein in Musca demestica and Anopheles darlingi showed highly homology. Conclusion: DmTTF protein in D. melanogaster contained the typical structure of mitochondrial transcription termination factor protein family, which might involve in the transcription regulation of mitochondrial gene of D. melanogaster.%目的:基于生物信息学方法分析黑腹果蝇线粒体转录终止因子DmTTF蛋白的结构和功能.方法:检索FlyBase数据库中黑腹果蝇线粒体转录终止因子DmTTF蛋白的氨基酸序列,利用不同生物信息学软件对该蛋白质的理化性质、亲水性/疏水性、亚细胞定位、二级结

  10. Background studies for a ton-scale argon dark matter detector (ArDM)

    CERN Document Server

    Kaufmann, L

    2006-01-01

    The ArDM project aims at operating a large noble liquid detector to search for direct evidence of Weakly Interacting Massive Particles (WIMP) as Dark Matter in the universe. Background sources relevant to ton-scale liquid and gaseous argon detectors, such as neutrons from detector components, muon-induced neutrons and neutrons caused by radioactivity of rock, as well as the internal $^{39}Ar$ background, are studied with simulations. These background radiations are addressed with the design of an appropriate shielding as well as with different background rejection potentialities. Among them the project relies on event topology recognition, event localization, density ionization discrimination and pulse shape discrimination. Background rates, energy spectra, characteristics of the background-induced nuclear recoils in liquid argon, as well as the shielding performance and rejection performance of the detector are described.

  11. STT Doubles with Large DM - Part V: Aquila, Delphinus, Cygnus, Aquarius

    Science.gov (United States)

    Knapp, Wilfried; Nanson, John

    2016-07-01

    The results of visual double star observing sessions suggested a pattern for STT doubles with large DM of being harder to resolve than would be expected based on the WDS catalog data. It was felt this might be a problem with expectations on one hand, and on the other might be an indication of a need for new precise measurements, so we decided to take a closer look at a selected sample of STT doubles and do some research. We found that, as in the other constellations covered so far (Gem, Leo, UMa etc.), at least several of the selected objects in Aql, Del, Cyg and Aqr show parameters quite different from the current WDS data

  12. DM9000AE及其在嵌入式以太网中的应用

    Institute of Scientific and Technical Information of China (English)

    黄惠群; 吴景东

    2006-01-01

    介绍了一种以ARM微处理器LPC2106为核心的嵌入式以太网接口设计方法.对系统软硬件设计进行了介绍.硬件部分介绍了系统的硬件组成以及网络控制器DM9000AE的性能和工作原理.系统软件设计介绍了IwIP TCP/IP协议的移植以及应该注意的问题.在此软硬件基础上完成了一种轻型的,性价比高的嵌入式以太网接口卡的设计.

  13. The design of red-blue 3D video fusion system based on DM642

    Science.gov (United States)

    Fu, Rongguo; Luo, Hao; Lv, Jin; Feng, Shu; Wei, Yifang; Zhang, Hao

    2016-10-01

    Aiming at the uncertainty of traditional 3D video capturing including camera focal lengths, distance and angle parameters between two cameras, a red-blue 3D video fusion system based on DM642 hardware processing platform is designed with the parallel optical axis. In view of the brightness reduction of traditional 3D video, the brightness enhancement algorithm based on human visual characteristics is proposed and the luminance component processing method based on YCbCr color space is also proposed. The BIOS real-time operating system is used to improve the real-time performance. The video processing circuit with the core of DM642 enhances the brightness of the images, then converts the video signals of YCbCr to RGB and extracts the R component from one camera, so does the other video and G, B component are extracted synchronously, outputs 3D fusion images finally. The real-time adjustments such as translation and scaling of the two color components are realized through the serial communication between the VC software and BIOS. The system with the method of adding red-blue components reduces the lost of the chrominance components and makes the picture color saturation reduce to more than 95% of the original. Enhancement algorithm after optimization to reduce the amount of data fusion in the processing of video is used to reduce the fusion time and watching effect is improved. Experimental results show that the system can capture images in near distance, output red-blue 3D video and presents the nice experiences to the audience wearing red-blue glasses.

  14. Changes in endotoxin levels in T2DM subjects on anti-diabetic therapies

    Directory of Open Access Journals (Sweden)

    Kumar Sudhesh

    2009-04-01

    Full Text Available Abstract Introduction Chronic low-grade inflammation is a significant factor in the development of obesity associated diabetes. This is supported by recent studies suggesting endotoxin, derived from gut flora, may be key to the development of inflammation by stimulating the secretion of an adverse cytokine profile from adipose tissue. Aims The study investigated the relationship between endotoxin and various metabolic parameters of diabetic patients to determine if anti-diabetic therapies exerted a significant effect on endotoxin levels and adipocytokine profiles. Methods Fasting blood samples were collected from consenting Saudi Arabian patients (BMI: 30.2 ± (SD5.6 kg/m2, n = 413, consisting of non-diabetics (ND: n = 67 and T2DM subjects (n = 346. The diabetics were divided into 5 subgroups based on their 1 year treatment regimes: diet-controlled (n = 36, metformin (n = 141, rosiglitazone (RSG: n = 22, a combined fixed dose of metformin/rosiglitazone (met/RSG n = 100 and insulin (n = 47. Lipid profiles, fasting plasma glucose, insulin, adiponectin, resistin, TNF-α, leptin, C-reactive protein (CRP and endotoxin concentrations were determined. Results Regression analyses revealed significant correlations between endotoxin levels and triglycerides (R2 = 0.42; p 2 = 0.10; p 2 = 0.076; p 2 = 0.032; p 2 = 0.055; p Conclusion We conclude that sub-clinical inflammation in T2DM may, in part, be mediated by circulating endotoxin. Furthermore, that whilst the endotoxin and adipocytokine profiles of diabetic patients treated with different therapies were comparable, the RSG group demonstrated significant differences in both adiponectin and endotoxin levels. We confirm an association between endotoxin and serum insulin and triglycerides and an inverse relationship with HDL. Lower endotoxin and higher adiponectin in the groups treated with RSG may be related and indicate another mechanism for the effect of RSG on insulin sensitivity.

  15. Retrogression of Nervous Fibers According to the Age of Patients with Diabetes Mellitus (DM).

    Science.gov (United States)

    Han, Dongwook

    2013-09-01

    [Purpose] This study was performed to discover the possible onset time of diabetic neuropathy by age of diabetic patients, and to provide the knowledge necessary for preventing or managing diabetic neuropathy. [Subjects] The subjects of this study were outpatients who visited D Hospital Department of Neurology with complaints of significant neuropathic symptoms including dullness, numbness and paraesthesia. [Methods] Stimulations of 5 Hz, 250 Hz and 2,000 Hz were generated with a Neurometer CPT (Neurotron Inc., Baltimore, MD, USA) and delivered selectively to C fibers, A-delta fibers and A-beta fibers. The intensity of the stimulations of 5 Hz, 250 Hz and 2,000 Hz was incrementally increased as much as 0.01 mA. [Result] The results of this experiment show that the period of retrogression of nervous fibers was different significantly according to the age of patients with diabetes mellitus. Especially, in the case of individuals in their 50's, Aβ, Aδ, and C fibers in both the right and left lower limbs significantly changed within a period of 2 months. In the case of individuals in their 60's, Aβ and C fibers of the right lower limb meaningfully changed 2 months after the onset of the disease, and Aβ, Aδ, and C fibers of the left lower limb also significantly changed within a period of 2 months. [Conclusion] We discovered that patients suffering from DM especially in their 50's or 60's should be thoroughly followed for their condition, right from the onset of DM, in order to prevent the retrogression of nervous fibers.

  16. Comparison of epsilon- and delta-class glutathione S-transferases: the crystal structures of the glutathione S-transferases DmGSTE6 and DmGSTE7 from Drosophila melanogaster.

    Science.gov (United States)

    Scian, Michele; Le Trong, Isolde; Mazari, Aslam M A; Mannervik, Bengt; Atkins, William M; Stenkamp, Ronald E

    2015-10-01

    Cytosolic glutathione transferases (GSTs) comprise a large family of enzymes with canonical structures that diverge functionally and structurally among mammals, invertebrates and plants. Whereas mammalian GSTs have been characterized extensively with regard to their structure and function, invertebrate GSTs remain relatively unstudied. The invertebrate GSTs do, however, represent potentially important drug targets for infectious diseases and agricultural applications. In addition, it is essential to fully understand the structure and function of invertebrate GSTs, which play important roles in basic biological processes. Invertebrates harbor delta- and epsilon-class GSTs, which are not found in other organisms. Drosophila melanogaster GSTs (DmGSTs) are likely to contribute to detoxication or antioxidative stress during development, but they have not been fully characterized. Here, the structures of two epsilon-class GSTs from Drosophila, DmGSTE6 and DmGSTE7, are reported at 2.1 and 1.5 Å resolution, respectively, and are compared with other GSTs to identify structural features that might correlate with their biological functions. The structures of DmGSTE6 and DmGSTE7 are remarkably similar; the structures do not reveal obvious sources of the minor functional differences that have been observed. The main structural difference between the epsilon- and delta-class GSTs is the longer helix (A8) at the C-termini of the epsilon-class enzymes.

  17. Distinct roles of the DmNav and DSC1 channels in the action of DDT and pyrethroids.

    Science.gov (United States)

    Rinkevich, Frank D; Du, Yuzhe; Tolinski, Josh; Ueda, Atsushi; Wu, Chun-Fang; Zhorov, Boris S; Dong, Ke

    2015-03-01

    Voltage-gated sodium channels (Nav channels) are critical for electrical signaling in the nervous system and are the primary targets of the insecticides DDT and pyrethroids. In Drosophila melanogaster, besides the canonical Nav channel, Para (also called DmNav), there is a sodium channel-like cation channel called DSC1 (Drosophila sodium channel 1). Temperature-sensitive paralytic mutations in DmNav (para(ts)) confer resistance to DDT and pyrethroids, whereas DSC1 knockout flies exhibit enhanced sensitivity to pyrethroids. To further define the roles and interaction of DmNav and DSC1 channels in DDT and pyrethroid neurotoxicology, we generated a DmNav/DSC1 double mutant line by introducing a para(ts1) allele (carrying the I265N mutation) into a DSC1 knockout line. We confirmed that the I265N mutation reduced the sensitivity to two pyrethroids, permethrin and deltamethrin of a DmNav variant expressed in Xenopus oocytes. Computer modeling predicts that the I265N mutation confers pyrethroid resistance by allosterically altering the second pyrethroid receptor site on the DmNav channel. Furthermore, we found that I265N-mediated pyrethroid resistance in para(ts1) mutant flies was almost completely abolished in para(ts1);DSC1(-/-) double mutant flies. Unexpectedly, however, the DSC1 knockout flies were less sensitive to DDT, compared to the control flies (w(1118A)), and the para(ts1);DSC1(-/-) double mutant flies were even more resistant to DDT compared to the DSC1 knockout or para(ts1) mutant. Our findings revealed distinct roles of the DmNav and DSC1 channels in the neurotoxicology of DDT vs. pyrethroids and implicate the exciting possibility of using DSC1 channel blockers or modifiers in the management of pyrethroid resistance.

  18. Preclinical safety profile of trastuzumab emtansine (T-DM1): Mechanism of action of its cytotoxic component retained with improved tolerability

    Energy Technology Data Exchange (ETDEWEB)

    Poon, Kirsten Achilles, E-mail: achilles.kirsten@gene.com [Genentech, Inc., South San Francisco, CA (United States); Flagella, Kelly; Beyer, Joseph [Genentech, Inc., South San Francisco, CA (United States); Tibbitts, Jay [UCB, Brussels (Belgium); Kaur, Surinder; Saad, Ola; Yi, Joo-Hee; Girish, Sandhya; Dybdal, Noel; Reynolds, Theresa [Genentech, Inc., South San Francisco, CA (United States)

    2013-12-01

    Trastuzumab emtansine (T-DM1) is the first antibody-drug conjugate (ADC) approved for patients with human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer. The therapeutic premise of ADCs is based on the hypothesis that targeted delivery of potent cytotoxic drugs to tumors will provide better tolerability and efficacy compared with non-targeted delivery, where poor tolerability can limit efficacious doses. Here, we present results from preclinical studies characterizing the toxicity profile of T-DM1, including limited assessment of unconjugated DM1. T-DM1 binds primate ErbB2 and human HER2 but not the rodent homolog c-neu. Therefore, antigen-dependent and non-antigen-dependent toxicity was evaluated in monkeys and rats, respectively, in both single- and repeat-dose studies; toxicity of DM1 was assessed in rats only. T-DM1 was well tolerated at doses up to 40 mg/kg (∼ 4400 μg DM1/m{sup 2}) and 30 mg/kg (∼ 6000 μg DM1/m{sup 2}) in rats and monkeys, respectively. In contrast, DM1 was only tolerated up to 0.2 mg/kg (1600 μg DM1/m{sup 2}). This suggests that at least two-fold higher doses of the cytotoxic agent are tolerated in T-DM1, supporting the premise of ADCs to improve the therapeutic index. In addition, T-DM1 and DM1 safety profiles were similar and consistent with the mechanism of action of DM1 (i.e., microtubule disruption). Findings included hepatic, bone marrow/hematologic (primarily platelet), lymphoid organ, and neuronal toxicities, and increased numbers of cells of epithelial and phagocytic origin in metaphase arrest. These adverse effects did not worsen with chronic dosing in monkeys and are consistent with those reported in T-DM1-treated patients to date. - Highlights: • T-DM1 was well tolerated in preclinical studies in rats and cynomolgus monkeys. • T-DM1 is associated with bone marrow/hematologic, hepatic, and neuronal toxicities. • T-DM1 toxicities are related to DM1 mechanisms of action and pharmacologic

  19. Diagnóstico molecular de la Distrofia Miotónica (DM en Costa Rica

    Directory of Open Access Journals (Sweden)

    Fernando Morales Montero

    2001-10-01

    Full Text Available La Distrofia Miotónica es una enfermedad multisistémica de herencia autosómica dominante. El defecto molecular es una expansión del trinucleótido CTG presente en la región 3' no codificante (3´UTR del gen DMPK, localizado en el cromosoma 19q13.3. El objetivo del estudio fue implementar el diagnóstico molecular de la Dm con el fin de contribuir a mejorar el manejo clínico de los pacientes afectados y a que el consejo genético sea más certero y preciso. El estudio se realizó en pacientes con diagnóstico mediante el uso de técnicas molecular, a saber, la hidridación de Southern y la PCR. Se obtuvo el diagnóstico molecular de 84 pacientes de 21 diferentes familias; en 34 se diagnosticó el defecto molecular. En 21 personas de familias donde se comprobó que segrega la mutación, el gen resultó normal. En 29 pacientes de ocho familias no se encontró la mutación. Se observó una correlación positiva entre la severidad de la enfermedad y el número de repeticiones CTG. Aquellos casos que resultaron negativos probablemente sean pacientes con mutaciones en otros genes, ya sea PROMM/DM2 u otras miotonias hereditarias. El diagnóstico molecular debe usarse como herramienta para logra la clasificación clínica de los pacientes. El abordaje correcto de la enfermedad, debido a que todavía no existe tratamiento, debe incluir, además del manejo clínico interdisciplinario, la prevención mediante el consejo genético basado en el diagnóstico molecular preciso de la condición de portador o portadora.

  20. The correlation of reproduction-related gene expression with germ cell number in DM and PLL gilts.

    Science.gov (United States)

    Hu, Yan-Biao; Pan, Zeng-Xiang; Xu, Dan; Xu, Yin-Xue; Liu, Hong-Lin; Huang, Rui-Hua; Hu, Zhi-Gang

    2006-09-01

    In this study, the ovarian germ cell number was counted in 3-week-old Duroc x Meishan (DM, n=30) and PIC x (Landrace x Large White) (PLL, n=53) gilts, and the mRNA expression levels of four reproduction-related genes were investigated by quantitative RT-PCR. Correlation of germ cell number with the expression level of these genes was analyzed. Results showed that the germ cell number of DM was significantly higher than that of PLL gilts (PPLL gilts (P=0.269). No significant correlation existed between germ cell number and ovarian weight in the two gilt groups (R=0.335, P=0.07; R=0.119, P=0.398, respectively). A significant correlation was found between the germ cell number and expression level of ESR and IGF1R mRNA in DM gilts (R=0.648, PPLL gilts (R=0.435, PPLL gilts.

  1. Beyond the standard $\\Lambda$CDM cosmology: the observed structure of DM halos and the shape of the power spectrum

    CERN Document Server

    Demiański, M

    2015-01-01

    To restore the evolutionary history of the Dark Matter (DM) dominated objects -- galaxies and clusters of galaxies. Analyze the observational data to reveal correlations between the virial mass, $M_{vir}$, of halos and main properties of their central cores, namely, the mean DM density, pressure and entropy, and the redshifts of halo formation, $z_f$. These correlations indicate a high degree of self similarity of both the process of halos formation and the internal structure of relaxed halos. We confirm the CDM--like shape of the small scale power spectrum. However our reconstruction of evolutionary history of observed objects differs from expectations of the standard $\\Lambda$CDM cosmology and requires either multicomponent composition of DM or more complex primordial power spectrum of density perturbations with significant excess of power at scales of clusters of galaxies and larger. This approach seems to be quite efficient and suitably supplements the current investigations of galaxies at large redshifts...

  2. Crystal Structure of Allophycocyanin from Marine Cyanobacterium Phormidium sp. A09DM.

    Science.gov (United States)

    Sonani, Ravi Raghav; Gupta, Gagan Deep; Madamwar, Datta; Kumar, Vinay

    2015-01-01

    Isolated phycobilisome (PBS) sub-assemblies have been widely subjected to X-ray crystallography analysis to obtain greater insights into the structure-function relationship of this light harvesting complex. Allophycocyanin (APC) is the phycobiliprotein always found in the PBS core complex. Phycocyanobilin (PCB) chromophores, covalently bound to conserved Cys residues of α- and β- subunits of APC, are responsible for solar energy absorption from phycocyanin and for transfer to photosynthetic apparatus. In the known APC structures, heterodimers of α- and β- subunits (known as αβ monomers) assemble as trimer or hexamer. We here for the first time report the crystal structure of APC isolated from a marine cyanobacterium (Phormidium sp. A09DM). The crystal structure has been refined against all the observed data to the resolution of 2.51 Å to Rwork (Rfree) of 0.158 (0.229) with good stereochemistry of the atomic model. The Phormidium protein exists as a trimer of αβ monomers in solution and in crystal lattice. The overall tertiary structures of α- and β- subunits, and trimeric quaternary fold of the Phormidium protein resemble the other known APC structures. Also, configuration and conformation of the two covalently bound PCB chromophores in the marine APC are same as those observed in fresh water cyanobacteria and marine red algae. More hydrophobic residues, however, constitute the environment of the chromophore bound to α-subunit of the Phormidium protein, owing mainly to amino acid substitutions in the marine protein.

  3. DM Ori: A Young Star Occulted by a Disturbance in Its Protoplanetary Disk

    Science.gov (United States)

    Rodriguez, Joseph E.; Stassun, Keivan G.; Cargile, Phillip; Shappee, Benjamin J.; Siverd, Robert J.; Pepper, Joshua; Lund, Michael B.; Kochanek, Christopher S.; James, David; Kuhn, Rudolf B.; Beatty, Thomas G.; Gaudi, B. Scott; Weintraub, David A.; Stanek, Krzysztof Z.; Holoien, Thomas W.-S.; Prieto, Jose L.; Feldman, Daniel M.; Espaillat, Catherine C.

    2016-11-01

    In some planet formation theories, protoplanets grow gravitationally within a young star’s protoplanetary disk, a signature of which may be a localized disturbance in the disk’s radial and/or vertical structure. Using time-series photometric observations by the Kilodegree Extremely Little Telescope South project and the All-Sky Automated Survey for SuperNovae, combined with archival observations, we present the discovery of two extended dimming events of the young star, DM Ori. This young system faded by ∼1.5 mag from 2000 March to 2002 August and then again in 2013 January until 2014 September (depth ∼1.7 mag). We constrain the duration of the 2000–2002 dimming to be 6 au from the host star, moving at ∼14.6 km s‑1 and is ∼4.9 au in width. This localized structure may indicate a disturbance such as that which may be caused by a protoplanet early in its formation.

  4. The Distribution and Chemistry of H$_2$CO in the DM Tau Protoplanetary Disk

    CERN Document Server

    Loomis, Ryan A; Öberg, Karin I; Guzman, Viviana V; Andrews, Sean M

    2015-01-01

    H$_2$CO ice on dust grains is an important precursor of complex organic molecules (COMs). H$_2$CO gas can be readily observed in protoplanetary disks and may be used to trace COM chemistry. However, its utility as a COM probe is currently limited by a lack of constraints on the relative contributions of two different formation pathways: on icy grain-surfaces and in the gas-phase. We use archival ALMA observations of the resolved distribution of H$_2$CO emission in the disk around the young low-mass star DM Tau to assess the relative importance of these formation routes. The observed H$_2$CO emission has a centrally peaked and radially broad brightness profile (extending out to 500 AU). We compare these observations with disk chemistry models with and without grain-surface formation reactions, and find that both gas and grain-surface chemistry are necessary to explain the spatial distribution of the emission. Gas-phase H$_2$CO production is responsible for the observed central peak, while grain-surface chemist...

  5. A White Light Megaflare on the dM4.5e Star YZ CMi

    CERN Document Server

    Kowalski, Adam F; Holtzman, Jon A; Wisniewski, John P; Hilton, Eric J

    2010-01-01

    On UT 2009 January 16, we observed a white light megaflare on the dM4.5e star YZ CMi as part of a long-term spectroscopic flare-monitoring campaign to constrain the spectral shape of optical flare continuum emission. Simultaneous U-band photometric and 3350A-9260A spectroscopic observations were obtained during 1.3 hours of the flare decay. The event persisted for more than 7 hours and at flare peak, the U-band flux was almost 6 magnitudes brighter than in the quiescent state. The properties of this flare mark it as one of the most energetic and longest-lasting white light flares ever to be observed on an isolated low-mass star. We present the U-band flare energetics and a flare continuum analysis. For the first time, we show convincingly with spectra that the shape of the blue continuum from 3350A to 4800A can be represented as a sum of two components: a Balmer continuum as predicted by the Allred et al radiative hydrodynamic flare models and a T ~ 10,000K blackbody emission component as suggested by many pr...

  6. A novel design of subminiature star sensor's imaging system based on TMS320DM3730

    Science.gov (United States)

    Liu, Meiying; Wang, Hu; Wen, Desheng; Yang, Shaodong

    2017-02-01

    Development of the next generation star sensor is tending to miniaturization, low cost and low power consumption, so the imaging system based on FPGA in the past could not meet its developing requirements. A novel design of digital imaging system is discussed in this paper. Combined with the MT9P031 CMOS image sensor's timing sequence and working mode, the sensor driving circuit and image data memory circuit were implemented with the main control unit TMS320DM3730. In order to make the hardware system has the advantage of small size and light weight, the hardware adopted miniaturization design. The software simulation and experimental results demonstrated that the designed imaging system was reasonable, the function of tunable integration time and selectable window readout modes were realized. The communication with computer was exact. The system has the advantage of the powerful image processing, small-size, compact, stable, reliable and low power consumption. The whole system volume is 40 mm *40 mm *40mm,the system weight is 105g, the system power consumption is lower than 1w. This design provided a feasible solution for the realization of the subminiature star sensor's imaging system.

  7. The Influence of Diabetic Experience against the Behavior of DM Controlling

    Directory of Open Access Journals (Sweden)

    Yusran Haskas

    2016-06-01

    Full Text Available The behavior control of diabetes mellitus can improve the life quality of patient through planning specific behaviors. The behavior can be reflected by the experience that diabetes itself or observed in surrounding. Therefore, this study was conducted to investigate the effect on behavior of diabetics experience against diabetes control. This type of study is explanatory research using cross sectional design. The collecting data was using diabetics experience related diabetes disease. The consecutive sampling technique was used to obtain 134 samples. Data were analyzed with chi square test. The results showed that the average 62.2% of diabetics to control diabetes well have a pleasant and unpleasant experience. Chi-square test results showed that diabetics experience have influence against the behavior diabetes control (p = 0.043. Findings influence on diabetics experience against the behavior control diabetes have implications for providing information and support positive consistently in order to become a source of knowledge and experience in improving the behavior good control of DM.

  8. Sensitive limits on the abundance of cold water vapor in the DM Tau protoplanetary disk

    CERN Document Server

    Bergin, E A; Brinch, C; Fogel, J; Yildiz, U A; Kristensen, L E; van~Dishoeck, E F; Bell, T A; Blake, G A; Cernicharo, J; Dominik, C; Lis, D; Melnick, G; Neufeld, D; Panic, O; Pearson, J C; Bachiller, R; Baudry, A; Benedettini, M; Benz, A O; Bjerkeli, P; Bontemps, S; Braine, J; Bruderer, S; Caselli, P; Codella, C; Daniel, F; di Giorgio, A M; Doty, S D; Encrenaz, P; Fich, M; Fuente, A; Giannini, T; Goicoechea, J R; de Graauw, Th; Helmich, F; Herczeg, G J; Herpin, F; Jacq, T; Johnstone, D; Jorgensen, J K; Larsson, B; Liseau, R; Marseille, M; McCoey, C; Nisini, B; Olberg, M; Parise, B; Plume, R; Risacher, C; Santiago-Garcia, J; Saraceno, P; Shipman, R; Tafalla, M; van Kempen, T A; Visser, R; Wampfler, S F; Wyrowski, F; van der Tak, F; Jellema, W; Tielens, A G G M; Hartogh, P; Stutzki, J; Szczerba, R

    2010-01-01

    We performed a sensitive search for the ground-state emission lines of ortho- and para-water vapor in the DM Tau protoplanetary disk using the Herschel/HIFI instrument. No strong lines are detected down to 3sigma levels in 0.5 km/s channels of 4.2 mK for the 1_{10}--1_{01} line and 12.6 mK for the 1_{11}--0_{00} line. We report a very tentative detection, however, of the 1_{10}--1_{01} line in the Wide Band Spectrometer, with a strength of T_{mb}=2.7 mK, a width of 5.6 km/s and an integrated intensity of 16.0 mK km/s. The latter constitutes a 6sigma detection. Regardless of the reality of this tentative detection, model calculations indicate that our sensitive limits on the line strengths preclude efficient desorption of water in the UV illuminated regions of the disk. We hypothesize that more than 95-99% of the water ice is locked up in coagulated grains that have settled to the midplane.

  9. ArDM: a ton-scale LAr detector for direct Dark Matter searches

    CERN Document Server

    AUTHOR|(CDS)2072225; Badertscher, A.; Boccone, V.; Bueno, A.; Carmona-Benitez, M.C.; Coleman, J.; Creus, W.; Curioni, A.; Daniel, M.; Dawe, E.J.; Degunda, U.; Gendotti, A.; Epprecht, L.; Horikawa, S.; Kaufmann, L.; Knecht, L.; Laffranchi, M.; Lazzaro, C.; Lightfoot, P.K.; Lussi, D.; Lozano, J.; Mavrokoridis, K.; Melgarejo, A.; Mijakowski, P.; Natterer, G.; Navas-Concha, S.; Otyugova, P.; de Prado, M.; Przewlocki, P.; Regenfus, C.; Resnati, F.; Robinson, M.; Rochet, J.; Romero, L.; Rondio, E.; Rubbia, A.; Scotto-Lavina, L.; Spooner, N.J.C.; Strauss, T.; Touramanis, C.; Ulbricht, J.; Viant, T.

    2011-01-01

    The Argon Dark Matter (ArDM-1t) experiment is a ton-scale liquid argon (LAr) double-phase time projection chamber designed for direct Dark Matter searches. Such a device allows to explore the low energy frontier in LAr with a charge imaging detector. The ionization charge is extracted from the liquid into the gas phase and there amplified by the use of a Large Electron Multiplier in order to reduce the detection threshold. Direct detection of the ionization charge with fine spatial granularity, combined with a measurement of the amplitude and time evolution of the associated primary scintillation light, provide powerful tools for the identification of WIMP interactions against the background due to electrons, photons and possibly neutrons if scattering more than once. A one ton LAr detector is presently installed on surface at CERN to fully test all functionalities and it will be soon moved to an underground location. We will emphasize here the lessons learned from such a device for the design of a large LAr ...

  10. DM Ori: A Young Star Occulted by a Disturbance in its Protoplanetary Disk

    CERN Document Server

    Rodriguez, Joseph E; Cargile, Phillip; Shappee, Benjamin J; Siverd, Robert J; Pepper, Joshua; Lund, Michael B; Kochanek, Christopher S; James, David; Kuhn, Rudolf B; Beatty, Thomas G; Gaudi, B Scott; Weintraub, David A; Stanek, Krzysztof Z; Holoien, Thomas W S; Prieto, Jose L; Feldman, Daniel M; Espaillat, Catherine C

    2016-01-01

    In some planet formation theories, protoplanets grow gravitationally within a young star's protoplanetary disk, a signature of which may be a localized disturbance in the disk's radial and/or vertical structure. Using time-series photometric observations by the Kilodegree Extremely Little Telescope South (KELT-South) project and the All-Sky Automated Survey for SuperNovae (ASAS-SN), combined with archival observations, we present the discovery of two extended dimming events of the young star, DM Ori. This young system faded by $\\sim$1.5 mag from 2000 March to 2002 August and then again in 2013 January until 2014 September (depth $\\sim$1.7 mag). We constrain the duration of the 2000-2002 dimming to be $$6 AU from the host star, moving at $\\sim$14.6 km/s, and is $\\sim$4.9 AU in width. This localized structure may indicate a disturbance such as may be caused by a protoplanet early in its formation.

  11. Faint dwarfs as a test of DM models: WDM vs. CDM

    CERN Document Server

    Governato, Fabio; Pontzen, Andrew; Loebman, Sarah; Reed, Darren; Brooks, Alyson M; Behroozi, Peter; Christensen, Charlotte; Madau, Piero; Mayer, Lucio; Shen, Sijing; Walker, Matthew; Quinn, Thomas; Wadsley, James

    2014-01-01

    We use high resolution Hydro$+$N-Body cosmological simulations to compare the assembly and evolution of a small field dwarf (stellar mass ~ 10$^{6-7}$ M$\\odot$, total mass 10$^{10}$ M$\\odot$ in $\\Lambda$ dominated CDM and 2keV WDM cosmologies. We find that star formation (SF) in the WDM model is reduced and delayed by 1-2 Gyr relative to the CDM model, independently of the details of SF and feedback. Independent of the DM model, but proportionally to the SF efficiency, gas outflows lower the central mass density through `dynamical heating', such that all realizations have circular velocities $<$ 20kms at 500$~$pc, in agreement with local kinematic constraints. As a result of dynamical heating, older stars are less centrally concentrated than younger stars, similar to stellar population gradients observed in nearby dwarf galaxies. We translate our simulations into artificial color-magnitude diagrams and star formation histories in order to directly compare to available observations. The simulated galaxies f...

  12. Reproducing properties of MW dSphs as descendants of DM-free TDGs

    CERN Document Server

    Yang, Yanbin; Fouquet, Sylvain; Flores, Hector; Puech, Mathieu; Pawlowski, Marcel S; Kroupa, Pavel

    2014-01-01

    The Milky Way (MW) dwarf spheroidal (dSph) satellites are known to be the most dark-matter (DM) dominated galaxies with estimates of dark to baryonic matter reaching even above one hundred. It comes from the assumption that dwarfs are dynamically supported by their observed velocity dispersions. However their spatial distributions around the MW is not at random and this could challenge their origin, previously assumed to be residues of primordial galaxies accreted by the MW potential. Here we show that alternatively, dSphs could be the residue of tidal dwarf galaxies (TDGs), which would have interacted with the Galactic hot gaseous halo and disk. TDGs are gas-rich and have been formed in a tidal tail produced during an ancient merger event at the M31 location, and expelled towards the MW. Our simulations show that low-mass TDGs are fragile to an interaction with the MW disk and halo hot gas. During the interaction, their stellar content is progressively driven out of equilibrium and strongly expands, leading ...

  13. 爱普生推出全方位家庭娱乐一体机EMP-DM1

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    5月23日,爱普生(中国)有限公司在北京举行了题为“随心动,随心映——爱普生私人FUN映机EMP—DM1”的新品发布会。作为一款入门级家用投影机,EMP—DM1采用了.all in one的设计理念,将投影机、音响、

  14. Beyond the standard $\\Lambda$CDM cosmology: the observed structure of DM halos and the shape of the power spectrum

    OpenAIRE

    Demiański, M.; Doroshkevich, A. G.

    2015-01-01

    Recent advances in observational astronomy allow to study various groups of Dark Matter (DM) dominated objects from the dwarf spheroidal (dSph) galaxies to clusters of galaxies that span the mass range from $10^{6} M_{\\odot}$ to $10^{15} M_{\\odot}$. To analyze data of this divers collection of objects we used a simple toy model of spherical DM halo formation that was initially proposed by Peebles. This model introduced the concept of the epoch or redshift of halo formation. Using this concept...

  15. Implementation of Hybrid DCT/DPCM Video-Encoder in the DM642 EVM for Educational Purposes

    Directory of Open Access Journals (Sweden)

    Jozef Huska

    2006-01-01

    Full Text Available There is presented implementation of hybrid DCT/DPCM video-encoder in the power ful TI’s media processor TMS320DM642 embed in evaluation module DM642 EVM. Code Composer Studio 3 is used as working space. Videoencoder is implemented with use of C code so it is easily understandable. The easiness is very important because the implemented video-encoder will be used for educational purposes. The structure of the encoder is lightly recognizable forstudents frequenting the courses Analog and Digital Television, Digital Image Processing and Encoding so some routines could lightly modified.

  16. Comparison of DAMA/LIBRA and DM ICE Results using Information Theory to Rule out Dark Matter Claims

    CERN Document Server

    Cline, David

    2015-01-01

    We study the details of the DAMA/LIBRA results and compare those with the recent published DM Ice results of ICE Cube. In various recent papers, it was shown that the 40K peak on DAMA/LIBRA data leaves no room for a Dark Matter signal in the bulk of the data. Using Information Theory for the different types of detection environments, we show that annual variation calculations and the DM Ice data reinforce the claims that the DAMA/LIBRA detector is not observing Dark Matter WIMPs.

  17. CTX Correlation to Disease Duration and Adiponectin in Egyptian Children with T1DM/ Korelacija između CTX-a i trajanja bolesti i adiponektina kod egipatske dece sa T1DM

    Directory of Open Access Journals (Sweden)

    Hashim Amel A.

    2016-01-01

    Full Text Available Uvod: U ovoj studiji istraživali smo odnos između adiponektina i markera koštanih promena kod egipatske dece i ado­lescenata sa T1DM, uticaj trajanja bolesti na ove markere, kao i potencijalne korelacije između adiponektina i ko­štanih markera kod ovih pacijenata.

  18. 在TMS320DM642上实现嵌入式TCP/IP协议栈%Embedded Solution of TCP/IP Protocol Stack Based on TMS320DM642

    Institute of Scientific and Technical Information of China (English)

    王平; 孙乐美

    2007-01-01

    实现了一个运行在数字信号处理器TMS320DM642上的嵌入式TCP/IP协议栈.对TCP/IP协议中的IP协议、ARP协议、UDP协议进行分析,完成基于TCP/IP协议的嵌入式网络系统.

  19. TMS320DM6467的视频转码监控系统设计%Design of Video Transcoding Monitoring System Based on TMS320DM6467

    Institute of Scientific and Technical Information of China (English)

    吴航; 林岩

    2012-01-01

    提出基于嵌入式Web服务器Boa和CGI技术的网络通信思想,以VPC6467视频转码系统为硬件平台,基于嵌入式Linux操作系统的编译开发环境,设计了视频转码设备的监控系统.详述了虚拟共享内存的进程通信机制、HTML页面与服务器通信的CGI技术、Linux系统交叉编译环境的搭建,以及监控系统各个模块的设计思想.%An network communication method is proposed based on embedded Web server Boa and CGI technology. Taking VPC6467 video transcoding system as hardware platform, the video transcoding monitoring system is realized based on compiling development environment of embedded Linux operating system. Process communication mechanism of virtual shared memory, CGI technique for communication between HTML pages and server,cross-compiling environment building in Linux system, and design ideas of all modules in the monitoring system are elaborated.

  20. Blood Pressure is Associated With Cerebral Blood Flow Alterations in Patients With T2DM as Revealed by Perfusion Functional MRI.

    Science.gov (United States)

    Xia, Wenqing; Rao, Hengyi; Spaeth, Andrea M; Huang, Rong; Tian, Sai; Cai, Rongrong; Sun, Jie; Wang, Shaohua

    2015-12-01

    Type 2 diabetes mellitus (T2DM) and hypertension are both associated with cognitive impairment and brain function abnormalities. We investigated whether abnormal cerebral blood flow (CBF) patterns exists in T2DM patients and possible relationships between aberrant CBF and cognitive performance. Furthermore, we examined the influence of hypertension on CBF alterations in T2DM patients. T2DM patients (n = 38) and non-T2DM subjects (n = 40) were recruited from clinics, hospitals, and normal community health screenings. Cerebral blood flow images were collected and analyzed using arterial spin labeling perfusion functional magnetic resonance imaging (fMRI). Regions with major CBF differences between T2DM patients and non-T2DM controls were detected via 1-way ANOVA. The interaction effects between hypertension and T2DM for CBF alterations were also examined. Correlation analyses illustrated the association between CBF values and cognitive performance and between CBF and blood pressure. Compared with non-T2DM controls, T2DM patients exhibited decreased CBF, primarily in the visual area and the default mode network (DMN); decreased CBF in these regions was correlated with cognitive performance. There was a significant interaction effect between hypertension and diabetes for CBF in the precuneus and the middle occipital gyrus. Additionally, blood pressure correlated negatively with CBF in T2DM patients.T2DM patients exhibited reduced CBF in the visual area and DMN. Hypertension may facilitate a CBF decrease in the setting of diabetes. T2DM patients may benefit from blood pressure control to maintain their brain perfusion through CBF preservation.

  1. Characterization and biocompatibility of glucan: a safe food additive from probiotic Lactobacillus plantarum DM5.

    Science.gov (United States)

    Das, Deeplina; Goyal, Arun

    2014-03-15

    Exopolysaccharide produced by lactic acid bacteria are the subject of an increasing number of studies for their potential applications in the food industry as stabilizing, bio-thickening and immunostimulating agents. In this regard, the authors isolated an exopolysaccharide producing probiotic lactic acid bacterium from fermented beverage Marcha of north eastern Himalayas. The isolate Lactobacillus plantarum DM5 showed extracellular glucansucrase activity of 0.48 U mg⁻¹ by synthesizing natural exopolysaccharide glucan (1.87 mg mL⁻¹) from sucrose. Zymogram analysis of purified enzyme confirms the presence of glucosyltransferase of approximately 148 kDa with optimal activity of 18.7 U mg⁻¹ at 30 °C and pH 5.4. The exopolysaccharide was purified by gel permeation chromatography and had an average molecular weight of 1.11 × 10⁶ Da. Acid hydrolysis and structural characterization of exopolysaccharide revealed that it was composed of d-glucose residues, containing 86.5% of α-(1→6) and 13.5% of α-(1→3) linkages. Rheological study exhibited a shear thinning effect of glucan appropriate for food additives. A cytotoxicity test of glucan on human embryonic kidney 293 (HEK 293) and human cervical cancer (HeLa) cell lines revealed its nontoxic biocompatible nature. This is the first report on the structure and biocompatibility of homopolysaccharide α-D-glucan (dextran) from probiotic Lactobacillus plantarum strain and its unique physical and rheological properties that facilitate its application in the food industry as viscosifying and gelling agent. © 2013 Society of Chemical Industry.

  2. THE DISTRIBUTION AND CHEMISTRY OF H{sub 2}CO IN THE DM TAU PROTOPLANETARY DISK

    Energy Technology Data Exchange (ETDEWEB)

    Loomis, Ryan A.; Öberg, Karin I.; Guzman, Viviana V. [Department of Astronomy, Harvard University, Cambridge, MA 02138 (United States); Cleeves, L. Ilsedore [Department of Astronomy, University of Michigan, Ann Arbor, MI 48109 (United States); Andrews, Sean M., E-mail: rloomis@cfa.harvard.edu [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States)

    2015-08-20

    H{sub 2}CO ice on dust grains is an important precursor of complex organic molecules (COMs). H{sub 2}CO gas can be readily observed in protoplanetary disks and may be used to trace COM chemistry. However, its utility as a COM probe is currently limited by a lack of constraints on the relative contributions of two different formation pathways: on icy grain surfaces and in the gas phase. We use archival Atacama Large (sub-)Millimeter Array observations of the resolved distribution of H{sub 2}CO emission in the disk around the young low-mass star DM Tau to assess the relative importance of these formation routes. The observed H{sub 2}CO emission has a centrally peaked and radially broad brightness profile (extending out to 500 AU). We compare these observations with disk chemistry models with and without grain-surface formation reactions and find that both gas and grain-surface chemistry are necessary to explain the spatial distribution of the emission. Gas-phase H{sub 2}CO production is responsible for the observed central peak, while grain-surface chemistry is required to reproduce the emission exterior to the CO snow line (where H{sub 2}CO mainly forms through the hydrogenation of CO ice before being non-thermally desorbed). These observations demonstrate that both gas and grain-surface pathways contribute to the observed H{sub 2}CO in disks and that their relative contributions depend strongly on distance from the host star.

  3. A Widespread, Clumpy Starburst in the Isolated Ongoing Dwarf Galaxy Merger dm1647+21

    Science.gov (United States)

    Privon, G. C.; Stierwalt, S.; Patton, D. R.; Besla, G.; Pearson, S.; Putman, M.; Johnson, K. E.; Kallivayalil, N.; Liss, S.; Titans, TiNy

    2017-09-01

    Interactions between pairs of isolated dwarf galaxies provide a critical window into low-mass hierarchical, gas-dominated galaxy assembly and the build-up of stellar mass in low-metallicity systems. We present the first Very Large Telescope/Multi Unit Spectroscopic Explorer (VLT/MUSE) optical integral field unit (IFU) observations of the interacting dwarf pair dm1647+21 selected from the TiNy Titans survey. The Hα emission is widespread and corresponds to a total unobscured star formation rate (SFR) of 0.44 M ⊙ yr‑1, which is 2.7 times higher than the SFR inferred from Sloan Digital Sky Survey (SDSS) data. The implied specific SFR (sSFR) for the system is elevated by more than an order of magnitude above non-interacting dwarfs in the same mass range. This increase is dominated by the lower-mass galaxy, which has a sSFR enhancement of >50. Examining the spatially resolved maps of classic optical line diagnostics, we find that the interstellar medium (ISM) excitation can be fully explained by star formation. The velocity field of the ionized gas is not consistent with simple rotation. Dynamical simulations indicate that the irregular velocity field and the stellar structure is consistent with the identification of this system as an ongoing interaction between two dwarf galaxies. The widespread, clumpy enhancements in the star formation in this system point to important differences in the effect of mergers on dwarf galaxies, compared to massive galaxies; rather than the funneling of gas to the nucleus and giving rise to a nuclear starburst, starbursts in low-mass galaxy mergers may be triggered by large-scale ISM compression, and thus may be more distributed.

  4. HLA-DM Polymorphism and Risk of Trichloroethylene Induced Medicamentosa-like Dermatitis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective To establish the association between genetic polymorphisms of HLA-DMA and HLA-DMB and risk of developing trichloroethylene-induced medicamentosa-like dermatitis (TIMLD). Methods Sixty-one cases were medically confirmed to have been affected with TIMLD and 60 controls were selected from exposed workers who were free from TIMLD.The TIMLD cases and controls were similar in terms of age, sex, and duration of exposure. DNA was extracted both from the TIMLD cases and controls, HLA-DMA and HLA-DMB loci were amplified by using Touchdown PCR, and the alleles and genotypes were confirmed by restriction fragment length polymorphism (RFLP) and direct sequencing. Finally, the frequencies of HLA-DMA and HLA-DMB variants were compared between the two groups. Results The results showed that the frequency of HLA-DMA*0101 and HLA-DMB*0103 alleles was significantly increased in TIMLD patients than in controls (71.3% vs. 55.0% for HLA-DMA*0101; P<0.05) (11.5% vs. 3.3% for HLA-DMB*0103; P<0.05). In addition, the frequency of HLA-DMA*0102-*0102 homozygous genotype was also significantly higher in the controls than in the patients (25.0% vs.8.2%, P<0.05), whereas the frequency of heterozygous HLA-DMB *0101-*0102 genotype was lower in the patients in comparison with the controls. Conclusion The polymorphisms of HLA-DM may be associated with the susceptibility to TIMLD.

  5. Plasma amino acid and metabolite signatures tracking diabetes progression in the UCD-T2DM rat model

    Science.gov (United States)

    Elevations of plasma concentrations of branched-chain amino acids (BCAAs) are observed in human insulin resistance and type 2 diabetes mellitus (T2DM); however, there has been some controversy with respect to the passive or causative nature of the BCAA phenotype. Using untargeted metabolomics, plasm...

  6. Detection of H2D+: Measuring the Midplane Degree of Ionization in the Disks of DM Tauri and TW Hydrae

    NARCIS (Netherlands)

    Ceccarelli, C.; Dominik, C.; Lefloch, B.; Caselli, P.; Caux, E.

    2004-01-01

    We report the first detection of the ground transition of the ortho-H2D+ molecule toward one disk source, DM Tau, a tentative detection toward TW Hya, and an upper limit toward LkCa 15. The three observed sources possess young gas-rich disks with large CO depletion factors. We argue that the observe

  7. Simplified DM models with the full SM gauge symmetry : the case of $t$-channel colored scalar mediators

    CERN Document Server

    Ko, P; Park, Myeonghun; Yokoya, Hiroshi

    2016-01-01

    The general strategy for dark matter (DM) searches at colliders currently relies on simplified models. In this paper, we propose a new $t$-channel UV-complete simplified model that improves the existing simplified DM models in two important respects: (i) we impose the full SM gauge symmetry including the fact that the left-handed and the right-handed fermions have two independent mediators with two independent couplings, and (ii) we include the renormalization group evolution when we derive the effective Lagrangian for DM-nucleon scattering from the underlying UV complete models by integrating out the $t$-channel mediators. The first improvement will introduce a few more new parameters compared with the existing simplified DM models. In this study we look at the effect this broader set of free parameters has on direct detection and the mono-$X$ + MET ($X$=jet,$W,Z$) signatures at 13 TeV LHC while maintaining gauge invariance of the simplified model under the full SM gauge group. We find that the direct detect...

  8. Prescription Pattern of Antihypertensive Agents in T2DM Patients Visiting Tertiary Care Centre in North India

    Directory of Open Access Journals (Sweden)

    Ethiraj Dhanaraj

    2012-01-01

    Full Text Available Background. Hypertension management is of a paramount importance in diabetic patients for cardiovascular risk reduction. Aim. To evaluate prescribing pattern of antihypertensive in T2DM (type 2 diabetes patients and compare with existing recent guidelines. Methods. A cross-sectional study involving evaluation of all T2DM patients referred to endocrinology unit at tertiary care centre for hypertension, comorbid complications, and recording prescription. Utilization of 5 different antihypertensive drug classes was compared for all patients receiving 1, 2, 3, 4, or more drugs. Logistical regression was used to assess likelihood of prescription of drugs and/or therapy for specific conditions mentioned in the guidelines. Results. Out of 1358, T2DM enrolled patients 1186 (87% had hypertension (males 52%, females 48%. The median duration (IQ of hypertension diabetics was 4 (1–10 years. A total of 25% patients had controlled BP and 75% with uncontrolled blood pressure (13% isolated systolic hypertension, 6% isolated diastolic hypertension, and 55% both elevated. Overall, ACE inhibitors (ACEIs were prescribed the highest (59% followed by angiotensin receptor blockers (ARBs (52%, calcium channel blockers (CCBs (29%, diuretics (27%, and beta-blockers (14%. Overall, 55% of T2DM patients were on polytherapy, 41% on monotherapy, and 4% had no antihypertensive treatment. Polytherapy was more predominant with age, duration of diabetes, duration of hypertension, and comorbid complications. Conclusion. Although prescribing pattern of antihypertensive showed adherence to existing evidence-based guidelines, higher proportion of uncontrolled hypertensive patients was found.

  9. Molecular, Physiological, and Motor Performance Defects in DMSXL Mice Carrying >1,000 CTG Repeats from the Human DM1 Locus

    OpenAIRE

    Aline Huguet; Fadia Medja; Annie Nicole; Alban Vignaud; Céline Guiraud-Dogan; Arnaud Ferry; Valérie Decostre; Jean-Yves Hogrel; Friedrich Metzger; Andreas Hoeflich; Martin Baraibar; Mário Gomes-Pereira; Jack Puymirat; Guillaume Bassez; Denis Furling

    2012-01-01

    Myotonic dystrophy type 1 (DM1) is caused by an unstable CTG repeat expansion in the 3'UTR of the DM protein kinase (DMPK) gene. DMPK transcripts carrying CUG expansions form nuclear foci and affect splicing regulation of various RNA transcripts. Furthermore, bidirectional transcription over the DMPK gene and non-conventional RNA translation of repeated transcripts have been described in DM1. It is clear now that this disease may involve multiple pathogenic pathways including changes in gene ...

  10. Comparative study on pharmacological effects of DM-phencynonate hydrochloride and its optical isomers

    Institute of Scientific and Technical Information of China (English)

    Li-yun WANG; Jian-quan ZHENG; Yun WANG; Bo-hua ZHONG; Jin-xiu RUAN; Ke-liang LIU

    2005-01-01

    Aim: The 3 -azabicyclo(3,3, 1)nonanyl-9-α-yl-α-cyclopentyl-α-phenyl-α-glycolate (DM-phencynonate hydrochloride, DMCPG) is a demethylated metabolite of 3-methyl-3-azabicyclo(3,3,1)nonanyl-9-α-yl-α-cyclopentyl-α-phenyl-α-glycolate (phencynonate hydrochloride, CPG). (±)DMCPG had one chiral center and two enantiomers [R(-) and S(+)DMCPG]. Here we carried out a comparative study of the pharmacological profiles of these optical isomers. Methods: Affinity and relative efficacy were tested using a radioligand-binding assay with muscarinic acetylcholine receptors from the rat cerebral cortex. Pharmacological activity was assessed in three individual experiments: (1) potentiating the effect of a subthreshold hypnotic dose of sodium pentobarbital; (2) inhibiting oxotremorineinduced salivation; and (3) inhibiting the contractile response to carbachol.Results: In the competitive binding assay, R(-)DMCPG (Ki=763.75 nmol/L) was 4-and 2-fold more potent than (±)DMCPG (Ki=3186 nmol/L) and S(+)DMCPG (Ki= 1699 nmol/L) in inhibiting the binding of [3H]QNB. The R(-) and S (+) configurations showed positive cooperation (nH>1) with the muscarinic receptor, whereas (±)DMCPG had a negative cooperation (nH<1) relationship with the muscarinic receptor in a radio-binding assay. Both the R(-) and S(+) configurations could potentiate the effect of sub-threshold hypnotic dose of sodium pentobarbital in a dose-dependent manner (the ED50 values were 2.53 and 18.65 mg/kg, respectively),but (±)DMCPG did not display significant central depressant effects at doses from 10 to 29.15 mg/kg (P>0.05). (±)DMCPG and its optical isomers suppressed the guinea pig ileum contractile response to carbachol. The IC50 values were 7.78×10-9,1.88×10-7, and 1.03× 10-7 nmol/L, respectively. In the anti-salivation study,(±)DMCPG and its enantiomers depressed oxotremorine- induced salivation in a dose-dependent manner, and the order of potency was R(-)DMCPG (ED50=0.44 mg/kg)>(±)DMCPG (ED50

  11. Attitudes and Barriers to Exercise in Adults with Type 1 Diabetes (T1DM) and How Best to Address Them: A Qualitative Study

    OpenAIRE

    Nadia Lascar; Amy Kennedy; Beverley Hancock; David Jenkins; Andrews, Robert C.; Sheila Greenfield; Parth Narendran

    2014-01-01

    BACKGROUND:Regular physical activity has recognised health benefits for people with T1DM. However a significant proportion of them do not undertake the recommended levels of activity. Whilst questionnaire-based studies have examined barriers to exercise in people with T1DM, a formal qualitative analysis of these barriers has not been undertaken. Our aims were to explore attitudes, barriers and facilitators to exercise in patients with T1DM.METHODOLOGY:A purposeful sample of long standing T1DM...

  12. Estimativa da entalpia reticular de adutos (DM Hm o utilizando-se formas modificadas da equação de Kapustinskii Estimating the value of lattice enthalpy (DM Hm o for adducts by using modified forms of Kapustinskii equation

    Directory of Open Access Journals (Sweden)

    Robson Fernandes de Farias

    1999-04-01

    Full Text Available In this work are presented two modified forms of Kapustinskii equation that could be used to estimate the values of the lattice enthalphies for adducts: DM Hm o=(-n.z+ .z- .10(2/D.(1-d*/D .K and DM Hm o=(-n.z+ .z-.10(2/d.(1-d*/d.K.d. Two new parameters related with steric effects and donor power of the ligands, J anddare introduced. The proposed equations were tested for 49 adducts (mainly from the zinc group halides. The difference between experimental (calorimetric and calculated values (using the proposed equations values are less than 5% for 41 of the tested adducts.

  13. Airplane's video image real-time compression system with TMS320DM642%基于TMS320DM642的机载视频图像实时压缩与处理系统

    Institute of Scientific and Technical Information of China (English)

    陈步康; 王元钦; 周成刚

    2011-01-01

    为了实现机载视频图像的高分辨率实时传输,设计了一种基于TMS320DM642 DSP的机载视频图像实时压缩与处理系统.在分析DM642芯片集成的片内外设特点基础上,设计了基于TMS320DM642 DSP的高集度的视频图像压缩处理平台,并采用基于C64X CPU的软件优化技术对DSP中MPEG-4压缩算法进行了优化,实现了对25帧/秒D1(704×576)或CIF(352×288)分辨率机载视频图像的实时压缩和恒定码率无线传输.%In order to transmit airplane's video image immediately and graphically, this paper designed an airplane's video image real-time MPEG-4 compression system which is based on the newest media processor TMS320DM642. Based on the analysis of the characteristic of the processor,the paper designed the hardware of the system. By using the optimization technique of the software which is based on C64X CPU,the system achieved the optimization of MPEG-4 arithmetic.The experiment shows that the compression system can real-timely compress 25 frames' D1 or CIF images per second according to the command , and transmit it with invariable rate.

  14. 基于TMS320DM6437的掌静脉图像采集系统设计%Design based on TMS320DM6437 palm vein image acquisition system

    Institute of Scientific and Technical Information of China (English)

    苑玮琦; 卢守波

    2015-01-01

    The design and implementation of a TMS320DM6437 to DSP as the core of the palm vein image acquisition system. This system adopts the wavelength of 470nm blue light and wavelength of 850nm near infrared light as the main light source and light source system by contactless palm hand imageacquisition mode, after the hand image acquisition, feature extraction ofinformation for the hand, and then combine the feature recognitionalgorithm for identification. Video processing subsystem of the system mainly consists of CMOS sensor, the TMS320DM6437 (VPSS) and display.Experimental results show that the image acquisition to the system clear,good system stability, fast acquisition speed, meet the requirement ofsystem design.%设计并实现了一种以TMS320DM6437 DSP为核心的掌静脉图像采集系统. 本系统采用了波长为470 nm蓝光和波长为850 nm近红外光作为主要光源,通过手掌和光源系统非接触的方式采集手部图像,采集完手部图像后,提取手部的特征信息,然后结合特征识别算法进行身份识别.该系统主要包括CMOS传感器、TMS320DM6437的视频处理子系统(VPSS)和显示器. 实验结果表明:该系统采集到的图像清晰、系统稳定性好、采集速度快,满足了系统的设计要求.

  15. 基于TMS320DM365的高速网络摄像机的设计%Design of high speed IP camera based on TMS320DM365

    Institute of Scientific and Technical Information of China (English)

    秦臻; 曹剑中

    2011-01-01

    Trditional network canmeras have many problems, such as complex configration, low effciency and high cost The design of high speed IP camera based on TMS320DM365 and embedded linux. Through the research of hardware and software desing of high speed IP camrea based on TMS320DM365. the video data is collected by writing driver program followingng V4L2standard from a front camera The video is compressed based on H.264 standard, then the compressed video data can be transported in 100M network follow the protocols of RTP and RTCP.%针对传统网络摄像杌的结构复杂、效率低下以及昂贵的成本等问题,提出了基于嵌入式Linux和达芬奇技术TMS320DM365处理器平台的高速网络摄像机系统的设计方案.通过对基于TMS320DM365的高速网络摄像机进行硬件软件设计,从前端摄像头采集视频数据,通过Linux操作系统的V4L2编写驱动程序从而实现视频的采集.经H.264压缩算法对视频数据进行处理,最后通过RTP以及RTCP编码后经由100 M网口完成网络传输.

  16. 神秘瑰丽的传说FRANCK MULLER 7008 DM T陀飞轮腕表

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    该腕表表盘弃用传统长短针,只以两个FRANCK MULLER专利的圆形转盘来显示时、分功能。7008 DM T陀飞轮腕表采用经典圆形表壳,与面盘上的时分指示盘以及不停舞动的陀飞轮形成完美呼应。

  17. Clarifying the role of the rostral dmPFC/dACC in fear/anxiety: learning, appraisal or expression?

    Directory of Open Access Journals (Sweden)

    Simon Maier

    Full Text Available Recent studies have begun to carve out a specific role for the rostral part of the dorsal medial prefrontal cortex (dmPFC and adjacent dorsal anterior cingulate cortex (dACC in fear/anxiety. Within a novel general framework of dorsal mPFC/ACC areas subserving the appraisal of threat and concomitant expression of fear responses and ventral mPFC/ACC areas subserving fear regulation, the rostral dmPFC/dACC has been proposed to specifically mediate the conscious, negative appraisal of threat situations including, as an extreme variant, catastrophizing. An alternative explanation that has not been conclusively ruled out yet is that the area is involved in fear learning. We tested two different fear expression paradigms in separate fMRI studies (study 1: instructed fear, study 2: testing of Pavlovian conditioned fear with independent groups of healthy adult subjects. In both paradigms the absence of reinforcement precluded conditioning. We demonstrate significant BOLD activation of an identical rostral dmPFC/dACC area. In the Pavlovian paradigm (study 2, the area only activated robustly once prior conditioning had finished. Thus, our data argue against a role of the area in fear learning. We further replicate a repeated observation of a dissociation between peripheral-physiological fear responding and rostral dmPFC/dACC activation, strongly suggesting the area does not directly generate fear responses but rather contributes to appraisal processes. Although we succeeded in preventing extinction of conditioned responding in either paradigm, the data do not allow us to definitively exclude an involvement of the area in fear extinction learning. We discuss the broader implications of this finding for our understanding of mPFC/ACC function in fear and in negative emotion more generally.

  18. HDL-C / apoA-I ] : a multi-vessel cardiometabolic risk marker in women with T2DM.

    Science.gov (United States)

    Hermans, Michel P; Valensi, Paul; Ahn, Sylvie A; Rousseau, Michel F

    2017-09-15

    Although women have higher HDL-C than men, their HDL particles are also prone to become small, dense and dysfunctional in case of T2DM. To assess the vascular risk related to HDLs of different sizes/densities without direct measurement, we adjusted HDL-C to its main apolipoprotein (apoA-I), as [HDL-C/apoA-I]. This ratio estimates HDLs size and provides indices as to their number, cholesterol load, and density. We stratified 280 Caucasian T2DM women according to [HDL-C/apoA-I] quartiles (Q), to determine how it segregates cardiometabolic risk, β-cell function, glycemic control, and vascular complications. Five parameters were derived from combined determination of HDL-C and apoA-I : HDL size; HDL number; cholesterol load per particle (pP); apoA-IpP; and HDL density. An adverse cardiometabolic profile characterized Q-I and Q-II patients, whose HDLs were denser and apoA-I-depleted, whereas Q-III patients had HDLs with characteristics closer to controls. Q-IV patients had HDLs of supernormal size/composition and a more favorable phenotype in terms of fat distribution; insulin sensitivity (64% vs 41%), metabolic syndrome, β-cell function (32% vs 23%); exogenous insulin (44 vs 89 U/day), and glycemic control (HbA1c: 56 vs 61 mmol/mol), associated with lower prevalence of micro-/macrovascular complications: all-cause microangiopathy 47% vs 61%; retinopathy 22% vs 34%; all-cause macroangiopathy 19% vs 31%; and CAD: 6% vs 24% (pHDL-C/apoA-I] can stratify T2DM women according to metabolic phenotype, macrovascular and coronary damage, β-cell function, microangiopathic risk, and retinopathy. This ratio is a versatile and readily available marker of cardiometabolic status and vascular complications in T2DM women. This article is protected by copyright. All rights reserved.

  19. Mice mutant in the DM domain gene Dmrt4 are viable and fertile but have polyovular follicles.

    Science.gov (United States)

    Balciuniene, Jorune; Bardwell, Vivian J; Zarkower, David

    2006-12-01

    Proteins containing the DM domain, a zinc finger-like DNA binding motif, have been implicated in sexual differentiation in diverse metazoan organisms. Of seven mammalian DM domain genes, only Dmrt1 and Dmrt2 have been functionally analyzed. Here, we report expression analysis and targeted disruption of Dmrt4 (also called DmrtA1) in the mouse. Dmrt4 is widely expressed during embryonic and postnatal development. However, we find that mice homozygous for a putative null mutation in Dmrt4 develop essentially normally, undergo full sexual differentiation in both sexes, and are fertile. We observed two potential mutant phenotypes in Dmrt4 mutant mice. First, ovaries of most mutant females have polyovular follicles, suggesting a role in folliculogenesis. Second, 25% of mutant males consistently exhibited copulatory behavior toward other males. We also tested potential redundancy between Dmrt4 and two other gonadally expressed DM domain genes, Dmrt1 and Dmrt7. We observed no enhancement of gonadal phenotypes in the double mutants, suggesting that these genes function independently in gonadal development.

  20. Balancing Hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif.

    Science.gov (United States)

    Bilioni, Aphrodite; Sánchez-Hernández, David; Callejo, Ainhoa; Gradilla, Ana-Citlali; Ibáñez, Carmen; Mollica, Emanuela; Carmen Rodríguez-Navas, M; Simon, Eléanor; Guerrero, Isabel

    2013-04-15

    Hedgehog can signal both at a short and long-range, and acts as a morphogen during development in various systems. We studied the mechanisms of Hh release and spread using the Drosophila wing imaginal disc as a model system for polarized epithelium. We analyzed the cooperative role of the glypican Dally, the extracellular factor Shifted (Shf, also known as DmWif), and the Immunoglobulin-like (Ig-like) and Fibronectin III (FNNIII) domain-containing transmembrane proteins, Interference hedgehog (Ihog) and its related protein Brother of Ihog (Boi), in the stability, release and spread of Hh. We show that Dally and Boi are required to prevent apical dispersion of Hh; they also aid Hh recycling for its release along the basolateral part of the epithelium to form a long-range gradient. Shf/DmWif on the other hand facilitates Hh movement restrained by Ihog, Boi and Dally, establishing equilibrium between membrane attachment and release of Hh. Furthermore, this protein complex is part of thin filopodia-like structures or cytonemes, suggesting that the interaction between Dally, Ihog, Boi and Shf/DmWif is required for cytoneme-mediated Hh distribution during gradient formation.

  1. Status of ArDM-1t: First observations from operation with a full ton-scale liquid argon target

    CERN Document Server

    Calvo, J; Daniel, M; Degunda, U; Di Luise, S; Epprecht, L; Gendotti, A; Horikawa, S; Knecht, L; Montes, B; Mu, W; Munoz, M; Murphy, S; Natterer, G; Nguyen, K; Nikolics, K; Periale, L; Regenfus, C; Romero, L; Rubbia, A; Santorelli, R; Sergiampietri, F; Sgalaberna, D; Viant, T; Wu, S

    2015-01-01

    ArDM-1t is the first operating ton-scale liquid argon detector for direct search of Dark Matter particles. Developed at CERN as Recognized Experiment RE18, the experiment has been approved in 2010 to be installed in the Spanish underground site LSC (Laboratorio Subterraneo de Canfranc). Under the label of LSC EXP-08-2010 the ArDM detector underwent an intensive period of technical completion and safety approval until the recent filling of the target vessel with almost 2 ton of liquid argon. This report describes the experimental achievements during commissioning of ArDM and the transition into a stage of first physics data taking in single phase operational mode. We present preliminary observations from this run. A first indication for the background discrimination power of LAr detectors at the ton-scale is shown. We present an outlook for completing the detector with the electric drift field and upgrade of the scintillation light readout system with novel detector modules based on SiPMs in order to improve t...

  2. Neutron background at the Canfranc Underground Laboratory and its contribution to the IGEX-DM dark matter experiment

    CERN Document Server

    Carmona, J M; García, E; Irastorza, I G; Luzón, G; Morales, A; Morales, J; De Solorzano, A O; Puimedón, J; Sarsa, M L; Villar, J A

    2004-01-01

    A quantitative study of the neutron environment in the Canfranc Underground Laboratory has been performed. The analysis is based on a complete set of simulations and, particularly, it is focused on the IGEX-DM dark matter experiment. The simulations are compared to the IGEX-DM low energy data obtained with different shielding conditions. The results of the study allow us to conclude, with respect to the IGEX-DM background, that the main neutron population, coming from radioactivity from the surrounding rock, is practically eliminated after the implementation of a suitable neutron shielding. The remaining neutron background (muon-induced neutrons in the shielding and in the rock) is substantially below the present background level thanks to the muon veto system. In addition, the present analysis gives us a further insight on the effect of neutrons in other current and future experiments at the Canfranc Underground Laboratory. The comparison of simulations with the body of data available has allowed to set the ...

  3. The Effect of ‘Locus of Control’ on the Diabetes Mellitus Patients’ Intention in Performing the Dm Control

    Directory of Open Access Journals (Sweden)

    Yusran Haskas

    2016-02-01

    Full Text Available The behavioral control of diabetes mellitus can improve the quality of life through specific behavior planning. In the theory of planned behavior, the intentions are needed to bring up a person's behavior. A person can act on his intentions if he has control over his behavior. To control the behavior, the role of locus of control is required. Therefore, the research was conducted to investigate the effect of locus of control on the intention of diabetes mellitus patients in performing the diabetes mellitus control. This type of research was explanatory research using cross sectional design. The instrument of diabetic locus of control scale was used to collect the data. Consecutive sampling technique was used to obtain 134 samples. The data were analyzed by simple linear regression. The findings showed that 88,1% of respondents tend to have an internal locus of control and 98,6% of respondents have a strong intention to control the DM. Simple linear regression test results indicate that the locus of control significantly affect the diabetic patients’ intention to perform the control (p=0,032. The findings of the effect of locus of control on the intention of DM patients have implications for the need of the provision of information and positive support consistently in order to become a source of a filler control center that initiated the emergence of a strong intention to conduct the DM control.

  4. Performance of the CellaVision ® DM96 system for detecting red blood cell morphologic abnormalities

    Directory of Open Access Journals (Sweden)

    Christopher L Horn

    2015-01-01

    Full Text Available Background: Red blood cell (RBC analysis is a key feature in the evaluation of hematological disorders. The gold standard light microscopy technique has high sensitivity, but is a relativity time-consuming and labor intensive procedure. This study tested the sensitivity and specificity of gold standard light microscopy manual differential to the CellaVision ® DM96 (CCS; CellaVision, Lund, Sweden automated image analysis system, which takes digital images of samples at high magnification and compares these images with an artificial neural network based on a database of cells and preclassified according to RBC morphology. Methods: In this study, 212 abnormal peripheral blood smears within the Calgary Laboratory Services network of hospital laboratories were selected and assessed for 15 different RBC morphologic abnormalities by manual microscopy. The same samples were reassessed as a manual addition from the instrument screen using the CellaVision ® DM96 system with 8 microscope high power fields (×100 objective and a 22 mm ocular. The results of the investigation were then used to calculate the sensitivity and specificity of the CellaVision ® DM96 system in reference to light microscopy. Results: The sensitivity ranged from a low of 33% (RBC agglutination to a high of 100% (sickle cells, stomatocytes. The remainder of the RBC abnormalities tested somewhere between these two extremes. The specificity ranged from 84% (schistocytes to 99.5% (sickle cells, stomatocytes. Conclusions: Our results showed generally high specificities but variable sensitivities for RBC morphologic abnormalities.

  5. Poor glycemic control impacts linear and non-linear dynamics of heart rate in DM type 2

    Directory of Open Access Journals (Sweden)

    Daniela Bassi

    2015-08-01

    Full Text Available INTRODUCTION: It is well known that type 2 diabetes mellitus (T2DM produces cardiovascular autonomic neuropathy (CAN, which may affect the cardiac autonomic modulation. However, it is unclear whether the lack of glycemic control in T2DM without CAN could impact negatively on cardiac autonomic modulation. Objective: To evaluate the relationship between glycemic control and cardiac autonomic modulation in individuals with T2DM without CAN. Descriptive, prospective and cross sectional study.METHODS: Forty-nine patients with T2DM (51±7 years were divided into two groups according to glycosylated hemoglobin (HbA1c: G1≤7% and G2>7.0%. Resting heart rate (HR and RR interval (RRi were obtained and calculated by linear (Mean iRR; Mean HR; rMSSD; STD RR; LF; HF; LF/HF, TINN and RR Tri, and non-linear (SD1; SD2; DFα1; DFα2, Shannon entropy; ApEn; SampEn and CD methods of heart rate variability (HRV. Insulin, HOMA-IR, fasting glucose and HbA1c were obtained by blood tests.RESULTS: G2 (HbA1c≤7% showed lower values for the mean of iRR; STD RR; RR Tri, TINN, SD2, CD and higher mean HR when compared with G1 (HbA1c > 7%. Additionally, HbA1c correlated negatively with mean RRi (r=0.28, p=0.044; STD RR (r=0.33, p=0.017; RR Tri (r=-0.35, p=0.013, SD2 (r=-0.39, p=0.004 and positively with mean HR (r=0.28, p=0.045. Finally, fasting glucose correlated negatively with STD RR (r=-0.36, p=0.010; RR Tri (r=-0.36, p=0.010; TINN (r=-0.33, p=0.019 and SD2 (r=-0.42, p=0.002.CONCLUSION: We concluded that poor glycemic control is related to cardiac autonomic modulation indices in individuals with T2DM even if they do not present cardiovascular autonomic neuropathy.

  6. Conformational lability in the class II MHC 310 helix and adjacent extended strand dictate HLA-DM susceptibility and peptide exchange

    Science.gov (United States)

    Painter, Corrie A.; Negroni, Maria P.; Kellersberger, Katherine A.; Zavala-Ruiz, Zarixia; Evans, James E.; Stern, Lawrence J.

    2011-01-01

    HLA-DM is required for efficient peptide exchange on class II MHC molecules, but its mechanism of action is controversial. We trapped an intermediate state of class II MHC HLA-DR1 by substitution of αF54, resulting in a protein with increased HLA-DM binding affinity, weakened MHC-peptide hydrogen bonding as measured by hydrogen-deuterium exchange mass spectrometry, and increased susceptibility to DM-mediated peptide exchange. Structural analysis revealed a set of concerted conformational alterations at the N-terminal end of the peptide-binding site. These results suggest that interaction with HLA-DM is driven by a conformational change of the MHC II protein in the region of the α-subunit 310 helix and adjacent extended strand region, and provide a model for the mechanism of DM-mediated peptide exchange. PMID:22084083

  7. Conformational lability in the class II MHC 310 helix and adjacent extended strand dictate HLA-DM susceptibility and peptide exchange.

    Science.gov (United States)

    Painter, Corrie A; Negroni, Maria P; Kellersberger, Katherine A; Zavala-Ruiz, Zarixia; Evans, James E; Stern, Lawrence J

    2011-11-29

    HLA-DM is required for efficient peptide exchange on class II MHC molecules, but its mechanism of action is controversial. We trapped an intermediate state of class II MHC HLA-DR1 by substitution of αF54, resulting in a protein with increased HLA-DM binding affinity, weakened MHC-peptide hydrogen bonding as measured by hydrogen-deuterium exchange mass spectrometry, and increased susceptibility to DM-mediated peptide exchange. Structural analysis revealed a set of concerted conformational alterations at the N-terminal end of the peptide-binding site. These results suggest that interaction with HLA-DM is driven by a conformational change of the MHC II protein in the region of the α-subunit 3(10) helix and adjacent extended strand region, and provide a model for the mechanism of DM-mediated peptide exchange.

  8. Commissioning of the ArDM experiment at the Canfranc underground laboratory: first steps towards a tonne-scale liquid argon time projection chamber for Dark Matter searches

    Science.gov (United States)

    Calvo, J.; Cantini, C.; Crivelli, P.; Daniel, M.; Di Luise, S.; Gendotti, A.; Horikawa, S.; Montes, B.; Mu, W.; Murphy, S.; Natterer, G.; Nguyen, K.; Periale, L.; Quan, Y.; Radics, B.; Regenfus, C.; Romero, L.; Rubbia, A.; Santorelli, R.; Sergiampietri, F.; Viant, T.; Wu, S.

    2017-03-01

    The Argon Dark Matter (ArDM) experiment consists of a liquid argon (LAr) time projection chamber (TPC) sensitive to nuclear recoils, resulting from scattering of hypothetical Weakly Interacting Massive Particles (WIMPs) on argon targets. With an active target mass of 850 kg ArDM represents an important milestone towards developments for large LAr Dark Matter detectors. Here we present the experimental apparatus currently installed underground at the Laboratorio Subterráneo de Canfranc (LSC), Spain. We show data on gaseous or liquid argon targets recorded in 2015 during the commissioning of ArDM in single phase at zero E-field (ArDM Run I). The data confirms the overall good and stable performance of the ArDM tonne-scale LAr detector.

  9. Regulation of DM-20 mRNA expression and intracellular translocation of glutathione-S-transferase pi isoform during oligodendrocyte differentiation in the adult rat spinal cord.

    Science.gov (United States)

    Kitada, Masaaki; Takeda, Kazuya; Dezawa, Mari

    2016-07-01

    We previously demonstrated that NG2-positive oligodendrocyte precursor cells (OPCs) do not express DM-20 mRNA and identified a distinct DM-20 mRNA-positive cell population expressing glutathione-S-transferase pi isoform (GST-pi) in the nucleus (GST-pi(Nuc)) of the adult rat spinal cord. As GST-pi intranuclear localization correlates with progenitor cell properties, we examined the differentiation status of this cell population under the intensive 5-bromo-2'-deoxyuridine (BrdU) administration method, consisting of intraperitoneal BrdU injections every 2 h for 48 h. We observed that a certain population of proliferating/proliferated cells expressed DM-20 mRNA, and sometimes two proliferating/proliferated cells were observed still attached to each other. We performed triple staining for BrdU, DM-20 mRNA, and NG2 and found pairs of neighboring BrdU-positive cells, which were considered to originate from the same progenitor cells and where both cells expressed DM-20 mRNA. Triple staining for BrdU, DM-20 mRNA, and GST-pi detected proliferating/proliferated cells exhibiting the GST-pi(Nuc)/DM-20 mRNA-positive expression pattern. These findings suggested the presence of a GST-pi(Nuc)/DM-20 mRNA-positive oligodendrocyte-lineage progenitor cell population in the adult rat spinal cord. However, we did not find any pair of neighboring BrdU-positive cells with this expression pattern. These observations collectively support the idea that GST-pi(Nuc)/DM-20 mRNA-expressing cells are the progeny of NG2-positive OPCs rather than a novel type of oligodendrocyte-lineage progenitor cells and that DM-20 mRNA expression is dynamically regulated during differentiation of OPCs into oligodendrocytes.

  10. FINAL REPORT INTEGRATED DM1200 MELTER TESTING USING AZ 102 AND C 106/AY-102 HLW SIMULANTS: HLW SIMULANT VERIFICATION VSL-05R5800-1 REV 0 6/27/05

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; BRANDYS M; KOT WK; PEGG IL

    2011-12-29

    The principal objectives of the DM1200 melter tests were to determine the effects of feed rheology, feed solid content, and bubbler configuration on glass production rate and off-gas system performance while processing the HLW AZ-101 and C-106/AY-102 feed compositions; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components, as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and perform pre- and post test inspections of system components. The specific objectives (including test success criteria) of this testing, along with how each objective was met, are outlined in a table. The data provided in this Final Report address the impacts of HLW melter feed rheology on melter throughput and validation of the simulated HLW melter feeds. The primary purpose of this testing is to further validate/verify the HLW melter simulants that have been used for previous melter testing and to support their continued use in developing melter and off-gas related processing information for the Project. The primary simulant property in question is rheology. Simulants and melter feeds used in all previous melter tests were produced by direct addition of chemicals; these feed tend to be less viscous than rheological the upper-bound feeds made from actual wastes. Data provided here compare melter processing for the melter feed used in all previous DM100 and DM1200 tests (nominal melter feed) with feed adjusted by the feed vendor (NOAH Technologies) to be more viscous, thereby simulating more closely the upperbounding feed produced from actual waste. This report provides results of tests that are described in the Test Plan for this work. The Test Plan is responsive to one of several test objectives covered in the WTP Test Specification for this work; consequently, only part of the scope described in the Test Specification was addressed in this particular Test Plan. For the purpose of

  11. Sf9细胞存在Dm0-like核纤层蛋白的证据%Evidence for the existence of Dm0-like Lamin in Sf9 cells

    Institute of Scientific and Technical Information of China (English)

    卫文强; 季少平; 张银燕

    2015-01-01

    为了确定Sf9细胞是否存在核纤层(lamina)及其性状,该文首先用已知的昆虫的核纤层蛋白(Lamin)的基因序列在Spodobase数据库搜索Sf9细胞的同源序列,并将推导的氨基酸序列与其他物种的同源蛋白进行比对.再利用抗果蝇Lamin Dm0抗体ADL67通过免疫印迹法(Western blotting)对Sf9细胞的蛋白裂解物进行检测,并通过免疫荧光技术(immunofluore-scence)对Sf9细胞进行染色.在Spodobase数据库搜索到1条Sf9细胞的Dm0-likelamin EST序列,同源比对显示它与其他物种的Lamin存在一定的同源性,尤其与家蚕、果蝇的同源性相对较高.免疫印迹结果表明Sf9细胞裂解物中存在大小约为70 ku的蛋白,免疫荧光检测表明Sf9细胞核周呈现阳性反应,这些特征与已知的其他物种的核纤层的性状相似.结果表明,Sf9细胞可能存在Dm0-like核纤层蛋白,可作为探讨杆状病毒核衣壳穿过核纤层的机制之依据.

  12. CMIN - herramienta case basada en CRISP-DM para el soporte de proyectos de minería de datos CMIN — a CRISP-DM-based case tool for supporting data mining projects

    Directory of Open Access Journals (Sweden)

    Mendoza Martha

    2010-12-01

    Full Text Available En este artículo se presenta la CMIN, una herramienta CASE (Computer Aided Software Engineering integrada (que soporta todas las fases de un proceso basada en CRISP-DM 1.0 (Cross – Industry Standard Process for Data Mining para soportar el desarrollo de proyectos de minería de datos.Primero se expone la funcionalidad general de CMIN, lo que incluye la gestión de procesos, plantillas y proyectos, y se destaca la capacidad de CMIN para realizar el seguimiento de los proyectos de una forma fácil e intuitiva y la manera como CMIN posibilita que el usuario incremente su conocimiento en el uso de CRISP-DM o de cualquier otro proceso que se defina en la herramienta a través de las ayudas e información que se ofrece en cada paso del proceso. Después, se detalla cómo CMIN permite enlazar en tiempo de ejecución (sin necesidad de volver a compilar la herramienta nuevos algoritmos de minería de datos que apoyen la labor de modelado (basada en un flujo de trabajo o workflow en un proyecto de minería de datos. Finalmente, se ofrecen los resultados de dos evaluaciones de la herramienta, las conclusiones y el trabajo futuro.This paper introduces CMIN, an integrated computer aided software engineering (CASE tool based on cross-industry standard process for data mining (CRISP-DM 1.0 designed to support carrying out data mining projects. It is “integrated” in the sense that it supports all phases of a process. A general overview of how CMIN works is presented first, including a treatment of processes, templates and project management. CMIN’s capacity for easily and intuitively monitoring projects is highlighted, as is the manner in which CMIN allows a user to increase knowledge regarding using CRISP-DM or any other process defined in the CASE tool through the help and information presented in each step. Next, it is shown how CMIN can bind new data mining algorithms in runtime (without the need to recompile the tool to support modelling tasks

  13. 基于DM6437的Adaboost人脸检测算法的设计与实现%Design and implementation of Adaboost face detection algorithm based on DM6437

    Institute of Scientific and Technical Information of China (English)

    倪福银

    2012-01-01

    研究并实现了基于DM6437的Adaboost人脸检测算法。在对相关的人脸检测算法研究的基础上,选择了适应能力强、错误率小的Adaboost算法,通过对输入样本进行Harr特征提取,从中选出最优的Haar特征,然后将训练得到的Haar特征转换成弱分类器,再将弱分类器优化组合成强分类器,最后形成级联强分类器用于人脸检测。通过OpenCV在计算机上仿真实现该算法’,完成了Adaboost人脸检测算法的DSP程序设计,在DM6437硬件平台上实现了人脸实时检测功能。结果表明,运用该算法能够有效地进行人脸检测,可用于工程实践。%The paper studies realizes the Adaboost face detection algorithm based on DM6437. After studying the relevant algo- rithms, the paper chooses Adaboost face detection algorithm which is adaptable and has small error rate. Firstly ,it extracts the Hart features of input samples and chooses optimal Harr features through training, and changes it into weak classifier. Then, it makes weak classifier optimized into strong classifier. Finally it forms cascade classifier which is used in face detection. It simulates Ad- aboost face detection algorithm through OpenCV on the computer. The paper completes the program design of DSP and realizes the function of real-time face detection in DM6437 hardware platform. Results show that this algorithm can be used to realize face de- tection efficiendy, and can be used in engineering practice.

  14. Synthesis and Characterization of Cottonseed Oil Fatty Acid Butylamide%DM6446平台下车道线检测系统设计与实现

    Institute of Scientific and Technical Information of China (English)

    王晓栋; 严菲; 许华荣

    2012-01-01

    为了克服传统的“驾驶员一汽车一道路”闭环汽车驾驶模式中存在的缺点,提高车辆行驶安全,提出一种基于ARM+DSP架构的嵌入式实时车道线检测系统。介绍该系统的总体架构和异构双核处理器的数据处理流程及系统核心算法的实现和优化方法.同时提出了针对DM6446异构双核开发平台的系统实现方案,并通过实验验证了系统的有效性.%Fatty acid butylamide was synthesized with fatty acid from cottonseed oil and butylamine used as raw materials in this article. Synthetic conditions of fatty acid butylamide were researched through orthogonal experiment. The optimum technological conditions were as following: reaction temperature 125 ~C, reaction time 3.0 h, amount of catalyst 1.5%, molar ratio of fatty acid and butylamine 10: 7. Conversion rate of hutylamine can reach 92.45% under optimum conditions. The'structure of product was confirmed by Fourier transform infrared spectrometer( FrIR/for identifying, th ,~ I. L..__.

  15. Development of an AP-FRET based analysis for characterizing RNA-protein interactions in myotonic dystrophy (DM1.

    Directory of Open Access Journals (Sweden)

    Shagufta Rehman

    Full Text Available Förster Resonance Energy Transfer (FRET microscopy is a powerful tool used to identify molecular interactions in live or fixed cells using a non-radiative transfer of energy from a donor fluorophore in the excited state to an acceptor fluorophore in close proximity. FRET can be a very sensitive tool to study protein-protein and/or protein-nucleic acids interactions. RNA toxicity is implicated in a number of disorders; especially those associated with expanded repeat sequences, such as myotonic dystrophy. Myotonic dystrophy (DM1 is caused by a (CTGn repeat expansion in the 3' UTR of the DMPK gene which results in nuclear retention of mutant DMPK transcripts in RNA foci. This results in toxic gain-of-function effects mediated through altered functions of RNA-binding proteins (e.g. MBNL1, hnRNPH, CUGBP1. In this study we demonstrate the potential of a new acceptor photobleaching assay to measure FRET (AP-FRET between RNA and protein. We chose to focus on the interaction between MBNL1 and mutant DMPK mRNA in cells from DM1 patients due to the strong microscopic evidence of their co-localization. Using this technique we have direct evidence of intracellular interaction between MBNL1 and the DMPK RNA. Furthermore using the AP-FRET assay and MBNL1 mutants, we show that all four zinc-finger motifs in MBNL1 are crucial for MBNL1-RNA foci interactions. The data derived using this new assay provides compelling evidence for the interaction between RNA binding proteins and RNA foci, and mechanistic insights into MBNL1-RNA foci interaction demonstrating the power of AP-FRET in examining RNA-Protein interactions in DM1.

  16. Age of onset of RNA toxicity influences phenotypic severity: evidence from an inducible mouse model of myotonic dystrophy (DM1).

    Science.gov (United States)

    Gladman, Jordan T; Mandal, Mahua; Srinivasan, Varadamurthy; Mahadevan, Mani S

    2013-01-01

    Myotonic dystrophy type 1 (DM1) is the most common muscular dystrophy in adults. It is caused by an expanded (CTG)n tract in the 3' UTR of the Dystrophia Myotonica Protein Kinase (DMPK) gene. This causes nuclear retention of the mutant mRNA into ribonuclear foci and sequestration of interacting RNA-binding proteins (such as muscleblind-like 1 (MBNL1)). More severe congenital and childhood-onset forms of the disease exist but are less understood than the adult disease, due in part to the lack of adequate animal models. To address this, we utilized transgenic mice over-expressing the DMPK 3' UTR as part of an inducible RNA transcript to model early-onset myotonic dystrophy. In mice in which transgene expression was induced during embryogenesis, we found that by two weeks after birth, mice reproduced cardinal features of myotonic dystrophy, including myotonia, cardiac conduction abnormalities, muscle weakness, histopathology and mRNA splicing defects. Notably, these defects were more severe than in adult mice induced for an equivalent period of exposure to RNA toxicity. Additionally, the utility of the model was tested by over-expressing MBNL1, a key therapeutic strategy being actively pursued for treating the disease phenotypes associated with DM1. Significantly, increased MBNL1 in skeletal muscle partially corrected myotonia and splicing defects present in these mice, demonstrating the responsiveness of the model to relevant therapeutic interventions. Furthermore, these results also represent the first murine model for early-onset DM1 and provide a tool to investigate the effects of RNA toxicity at various stages of development.

  17. Age of onset of RNA toxicity influences phenotypic severity: evidence from an inducible mouse model of myotonic dystrophy (DM1.

    Directory of Open Access Journals (Sweden)

    Jordan T Gladman

    Full Text Available Myotonic dystrophy type 1 (DM1 is the most common muscular dystrophy in adults. It is caused by an expanded (CTGn tract in the 3' UTR of the Dystrophia Myotonica Protein Kinase (DMPK gene. This causes nuclear retention of the mutant mRNA into ribonuclear foci and sequestration of interacting RNA-binding proteins (such as muscleblind-like 1 (MBNL1. More severe congenital and childhood-onset forms of the disease exist but are less understood than the adult disease, due in part to the lack of adequate animal models. To address this, we utilized transgenic mice over-expressing the DMPK 3' UTR as part of an inducible RNA transcript to model early-onset myotonic dystrophy. In mice in which transgene expression was induced during embryogenesis, we found that by two weeks after birth, mice reproduced cardinal features of myotonic dystrophy, including myotonia, cardiac conduction abnormalities, muscle weakness, histopathology and mRNA splicing defects. Notably, these defects were more severe than in adult mice induced for an equivalent period of exposure to RNA toxicity. Additionally, the utility of the model was tested by over-expressing MBNL1, a key therapeutic strategy being actively pursued for treating the disease phenotypes associated with DM1. Significantly, increased MBNL1 in skeletal muscle partially corrected myotonia and splicing defects present in these mice, demonstrating the responsiveness of the model to relevant therapeutic interventions. Furthermore, these results also represent the first murine model for early-onset DM1 and provide a tool to investigate the effects of RNA toxicity at various stages of development.

  18. ANAEMIA AS A RISK FACTOR FOR MICROVASCULAR COMPLICATIONS IN TYPE 2 DM- A CROSS-SECTIONAL STUDY

    Directory of Open Access Journals (Sweden)

    Kamanuru Ethirajulu Govindarajulu

    2016-11-01

    Full Text Available BACKGROUND It is well known that diabetes adversely affects the kidneys finally leading to anaemia by various mechanisms. Several studies had postulated that anaemia developing before renal complications has an independent association with microvascular complication in type 2 diabetic patients. The aim of the study is to estimate the prevalence of anaemia in persons with type 2 diabetes mellitus and its role as a risk factor for the presence and the severity of microvascular complication in a populationbased study. MATERIALS AND METHODS This is a cross-sectional study conducted in patients coming to OPD of the Department of General Medicine in Government Vellore Medical College for a duration of 3 months from June 01, 2016, to August 31, 2016. Type 2 DM patients between the age group 20-60 years attending our diabetic clinic of both sex were included in our study. RESULTS From a total of 100 patients, 41% had anaemia including 34% with normochromic normocytic, 65.85% with hyperchromic microcytic anaemia and none of the patient had macrocytic anaemia. Patients who are anaemic had more frequent microvascular complications. There was no significant difference between males and females. The average duration of diabetes has a positive correlation with anaemia. All the microvascular complications like neuropathy, nephropathy and retinopathy had significant association with the presence of anaemia in type 2 patients. Nephropathy had a significant higher frequency compared to others as a complication in type 2 DM. CONCLUSION Our study shows that there is increased prevalence of anaemia in type 2 DM patients and the prevalence of microvascular complications is significantly higher among the diabetic patients with anaemia.

  19. Highly stable 2D material (2DM) field-effect transistors (FETs) with wafer-scale multidyad encapsulation

    Science.gov (United States)

    Kim, Choong-Ki; Gyo Jeong, Eun; Kim, Eungtaek; Song, Jeong-Gyu; Kim, Youngjun; Woo, Whang Je; Lee, Myung Keun; Bae, Hagyoul; Jeon, Seong-Bae; Kim, Hyungjun; Choi, Kyung Cheol; Choi, Yang-Kyu

    2017-02-01

    Field-effect transistors (FETs) composed of 2D materials (2DMs) such as transition-metal dichalcogenide (TMD) materials show unstable electrical characteristics in ambient air due to the high sensitivity of 2DMs to water adsorbates. In this work, in order to demonstrate the long-term retention of electrical characteristics of a TMD FET, a multidyad encapsulation method was applied to a MoS2 FET and thereby its durability was warranted for one month. It was well known that the multidyad encapsulation method was effective to mitigate high sensitivity to ambient air in light-emitting diodes (LEDs) composed of organic materials. However, there was no attempt to check the feasibility of such a multidyad encapsulation method for 2DM FETs. It is timely to investigate the water vapor transmission ratio (WVTR) required for long-term stability of 2DM FETs. The 2DM FETs were fabricated with MoS2 flakes by both an exfoliation method, that is desirable to attain high quality film, and a chemical vapor deposition (CVD) method, that is applicable to fabrication for a large-sized substrate. In order to eliminate other unwanted variables, the MoS2 FETs composed of exfoliated flakes were primarily investigated to assure the effectiveness of the encapsulation method. The encapsulation method uses multiple dyads comprised of a polymer layer by spin coating and an Al2O3 layer deposited by atomic layer deposition (ALD). The proposed method shows wafer-scale uniformity, high transparency, and protective barrier properties against adsorbates (WVTR of 8 × 10-6 g m-2 day-1) over one month.

  20. [Development of a multimedia learning DM diet education program using standardized patients and analysis of its effects on clinical competency and learning satisfaction for nursing students].

    Science.gov (United States)

    Hyun, Kyung Sun; Kang, Hyun Sook; Kim, Won Ock; Park, Sunhee; Lee, Jia; Sok, Sohyune

    2009-04-01

    The purpose of this study was to develop a multimedia learning program for patients with diabetes mellitus (DM) diet education using standardized patients and to examine the effects of the program on educational skills, communication skills, DM diet knowledge and learning satisfaction. The study employed a randomized control posttest non-synchronized design. The participants were 108 third year nursing students (52 experimental group, 56 control group) at K university in Seoul, Korea. The experimental group had regular lectures and the multimedia learning program for DM diet education using standardized patients while the control group had regular lectures only. The DM educational skills were measured by trained research assistants. The students who received the multimedia learning program scored higher for DM diet educational skills, communication skills and DM diet knowledge compared to the control group. Learning satisfaction of the experimental group was higher than the control group, but statistically insignificant. Clinical competency was improved for students receiving the multimedia learning program for DM diet education using standardized patients, but there was no statistically significant effect on learning satisfaction. In the nursing education system there is a need to develop and apply more multimedia materials for education and to use standardized patients effectively.

  1. Perspectives on Peripheral Neuropathy as a Consequence of Metformin-Induced Vitamin B12 Deficiency in T2DM

    Directory of Open Access Journals (Sweden)

    Marwan A. Ahmed

    2017-01-01

    Full Text Available Peripheral neuropathy (PN is a primary complication of type 2 diabetes mellitus (T2DM and a direct manifestation of vitamin B12 deficiency. Examining the effects of metformin use on PN status became imperative following clinical studies that showed the vitamin B12-lowering effect of the medication. The complexity of the topic and the inconsistency of the results warrant consideration of topic-specific perspectives for better understanding of the available evidence and more appropriate design of future studies.

  2. Depomed公司宣布DM-1796的Ⅲ期试验获重要结果

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Depomed公司宣布了一项Ⅲ期临床试验的重要结果,说明采用FDA规定的基线值代替缺失值(baseline observation carried forward,BOCF)的评价方法,DM-1796(或者叫做gabapentin ER)(Ⅰ)与安慰剂相比使带状疱疹后神经痛(pos therpetic neuralgia,PHN)有统计学意义上的显著减轻。

  3. 基于TMS320DM642实时手势识别系统的研究%Study on real-time gesture recognition system based on TMS320DM642

    Institute of Scientific and Technical Information of China (English)

    周小勇; 马军山; 李晓沛

    2011-01-01

    To satisfy the demands of hand gesture recognition in human-computer interaction system, a design method of real-time gesture recognition system based on TMS320DM642 is proposed. The system is composed by these function module groups: the gesture image acquisition modules and the gesture image processing and recognition modules. The experimental results show that the system works frequency is fast enough to realize the processing and recognition function via hand gesture pretreatment, skin segmentation, feature extraction and recognition. It is appropriate for the hand gesture recognition in human-computer interaction system.%为满足人机交互系统中手势识别与理解的需要,提出了一种基于TMS320DM642的实时手势识别系统.整个系统由手势图像采集、手势图像处理和匹配识别组成.实验结果表明,该系统通过手势图像预处理、肤色分割、特征提取及识别等算法的实现,能正确识别待测的手势图像,其速度可以满足后端处理的需要,可为下一步人机交互系统中手势的理解和识别打下良好的基础.

  4. 基于S3C2440的DM9000网卡驱动的移植%Transplantation of DM9000 network driver based on S3C2440

    Institute of Scientific and Technical Information of China (English)

    杨阳

    2014-01-01

    主要研究了基于 Linux 内核的网卡驱动的移植。 Linux 网络设备驱动程序的体系结构可以分为4层,首先分析了各层的具体功能实现,并在此基础上充分利用 S3C2440开发板完成 DM9000网卡驱动的移植,编译内核生成内核镜像。最后介绍了网络驱动测试的三种方法,并采用第一种方法,即使用 ifconfig 进行 IP 地址的设置,完成了测试。%This paper mainly studies the transplantation of network driver based on the Linux kernel. The architecture of Linux network device driver can be divided into four layers. Firstly, this paper analyzes the specific function implementation of each layer. And on this basis, it completes the transplantation of DM9000 network driver by making full use of the S3C2440 development board. Then it compiles the kernel to generate the kernel image. Finally, it introduces three methods of network drive test, and chooses the first one which uses command ifconfig to set IP address to finish the test.

  5. Separation of bacterial spores from flowing water in macro-scale cavities by ultrasonic standing waves

    CERN Document Server

    Lipkens, B; Costolo, M; Stevens, A; Rietman, Edward

    2010-01-01

    The separation of micron-sized bacterial spores (Bacillus cereus) from a steady flow of water through the use of ultrasonic standing waves is demonstrated. An ultrasonic resonator with cross-section of 0.0254 m x 0.0254 m has been designed with a flow inlet and outlet for a water stream that ensures laminar flow conditions into and out of the resonator section of the flow tube. A 0.01905-m diameter PZT-4, nominal 2-MHz transducer is used to generate ultrasonic standing waves in the resonator. The acoustic resonator is 0.0356 m from transducer face to the opposite reflector wall with the acoustic field in a direction orthogonal to the water flow direction. At fixed frequency excitation, spores are concentrated at the stable locations of the acoustic radiation force and trapped in the resonator region. The effect of the transducer voltage and frequency on the efficiency of spore capture in the resonator has been investigated. Successful separation of B. cereus spores from water with typical volume flow rates of...

  6. The divining root: moisture-driven responses of roots at the micro- and macro-scale.

    Science.gov (United States)

    Robbins, Neil E; Dinneny, José R

    2015-04-01

    Water is fundamental to plant life, but the mechanisms by which plant roots sense and respond to variations in water availability in the soil are poorly understood. Many studies of responses to water deficit have focused on large-scale effects of this stress, but have overlooked responses at the sub-organ or cellular level that give rise to emergent whole-plant phenotypes. We have recently discovered hydropatterning, an adaptive environmental response in which roots position new lateral branches according to the spatial distribution of available water across the circumferential axis. This discovery illustrates that roots are capable of sensing and responding to water availability at spatial scales far lower than those normally studied for such processes. This review will explore how roots respond to water availability with an emphasis on what is currently known at different spatial scales. Beginning at the micro-scale, there is a discussion of water physiology at the cellular level and proposed sensory mechanisms cells use to detect osmotic status. The implications of these principles are then explored in the context of cell and organ growth under non-stress and water-deficit conditions. Following this, several adaptive responses employed by roots to tailor their functionality to the local moisture environment are discussed, including patterning of lateral root development and generation of hydraulic barriers to limit water loss. We speculate that these micro-scale responses are necessary for optimal functionality of the root system in a heterogeneous moisture environment, allowing for efficient water uptake with minimal water loss during periods of drought. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  7. Macro-scale pseudo-particle modeling for particle-fluid systems

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Pseudo-particle modeling (PPM) is a particle method (PM) proposed in 1996. Though it is effective for the simulation of microscopic particle-fluid systems, its application to practical systems is still limited by computational cost.In this note, we speed up the computation by using a combination of weighted averaging with finite difference techniques to upgrade the particle interactions to a fluid element level, which conforms to the Navier-Stokes equation. The approach, abbreviated to MaPPM, is then applied to the problem of one-dimensional Poiseuille flow with a quantitative comparison to the results of another related PM smoothed particle hydrodynamics (SPH), where the accuracy and efficiency of MaPPM is found to be much better than that of SPH. Flows around a cylinder and multiple freely moving particles are also simulated with the new model, resulting in reasonable flow pattern and drag coefficient. The convergence and robustness of the algorithm prove promising.``

  8. Population vulnerability to biannual cholera outbreaks and associated macro-scale drivers in the Bengal Delta.

    Science.gov (United States)

    Akanda, Ali Shafqat; Jutla, Antarpreet S; Gute, David M; Sack, R Bradley; Alam, Munirul; Huq, Anwar; Colwell, Rita R; Islam, Shafiqul

    2013-11-01

    The highly populated floodplains of the Bengal Delta have a long history of endemic and epidemic cholera outbreaks, both coastal and inland. Previous studies have not addressed the spatio-temporal dynamics of population vulnerability related to the influence of underlying large-scale processes. We analyzed spatial and temporal variability of cholera incidence across six surveillance sites in the Bengal Delta and their association with regional hydroclimatic and environmental drivers. More specifically, we use salinity and flood inundation modeling across the vulnerable districts of Bangladesh to test earlier proposed hypotheses on the role of these environmental variables. Our results show strong influence of seasonal and interannual variability in estuarine salinity on spring outbreaks and inland flooding on fall outbreaks. A large segment of the population in the Bengal Delta floodplains remain vulnerable to these biannual cholera transmission mechanisms that provide ecologic and environmental conditions for outbreaks over large geographic regions.

  9. Silicon crystal growth from the melt: Analysis from atomic and macro scales

    Energy Technology Data Exchange (ETDEWEB)

    Kakimoto, K.; Liu, L.; Kitashima, T.; Murakawa, A.; Hashimoto, Y. [Research Institute for Applied Mechanics, Kyushu University, 6-1, Kasuga-Koen, Kasuga 816-8580 (Japan)

    2005-04-01

    The effect of impurity concentration on thermal conductivity of natural and isotope silicon by using equilibrium molecular dynamics simulation is investigated. It was found that the concentrations of the impurities such as boron, phosphor and arsene play an important role in the propagation of phonons in silicon crystals. It was also clarified that a mass difference of impurities and host crystals results in degradation of thermal conductivity of silicon. (copyright 2005 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  10. Data driven clustering of rain events: microphysics information derived from macro scale observations

    OpenAIRE

    Dilmi, Mohamed Djallel; Mallet, Cécile; Barthes, Laurent; Chazottes, Aymeric

    2016-01-01

    The study of rain time series records is mainly carried out using rainfall rate or rain accumulation parameters estimated on a fixed duration (typically 1 min, 1 hour or 1 day). In this paper we used the concept of rain event. Among the numerous existing variables dedicated to the characterisation of rain events, the first part of this paper aims to obtain a parsimonious characterisation of these events using a minimal set of variables. In this context an algorithm based on Genetic Algorithm ...

  11. Predator-prey interactions as macro-scale drivers of species diversity in mammals

    DEFF Research Database (Denmark)

    Sandom, Christopher James; Sandel, Brody Steven; Dalby, Lars

    -down). We gathered distributional range, mass and diet data for 4,091 terrestrial mammal species, excluding bats. Species richness maps were created for predators and prey and structural equation modelling was used to test the three hypotheses at continental and global scales. We also explored...

  12. Macro-Scale Patterns in Upwelling/Downwelling Activity at North American West Coast

    Science.gov (United States)

    Saldívar-Lucio, Romeo; Di Lorenzo, Emanuele; Nakamura, Miguel; Villalobos, Héctor; Lluch-Cota, Daniel; Del Monte-Luna, Pablo

    2016-01-01

    The seasonal and interannual variability of vertical transport (upwelling/downwelling) has been relatively well studied, mainly for the California Current System, including low-frequency changes and latitudinal heterogeneity. The aim of this work was to identify potentially predictable patterns in upwelling/downwelling activity along the North American west coast and discuss their plausible mechanisms. To this purpose we applied the min/max Autocorrelation Factor technique and time series analysis. We found that spatial co-variation of seawater vertical movements present three dominant low-frequency signals in the range of 33, 19 and 11 years, resembling periodicities of: atmospheric circulation, nodal moon tides and solar activity. Those periodicities might be related to the variability of vertical transport through their influence on dominant wind patterns, the position/intensity of pressure centers and the strength of atmospheric circulation cells (wind stress). The low-frequency signals identified in upwelling/downwelling are coherent with temporal patterns previously reported at the study region: sea surface temperature along the Pacific coast of North America, catch fluctuations of anchovy Engraulis mordax and sardine Sardinops sagax, the Pacific Decadal Oscillation, changes in abundance and distribution of salmon populations, and variations in the position and intensity of the Aleutian low. Since the vertical transport is an oceanographic process with strong biological relevance, the recognition of their spatio-temporal patterns might allow for some reasonable forecasting capacity, potentially useful for marine resources management of the region. PMID:27893826

  13. Characterizing pesticide sorption and degradation in macro scale biopurification systems using column displacement experiments.

    Science.gov (United States)

    De Wilde, Tineke; Spanoghe, Pieter; Mertens, Jan; Sniegowksi, Kristel; Ryckeboer, Jaak; Jaeken, Peter; Springael, Dirk

    2009-04-01

    The efficiency of biopurification systems to treat pesticide-contaminated water was previously studied in microcosms. To validate the obtained results, macrocosm systems were set-up. Four pesticides (linuron, isoproturon, bentazone, and metalaxyl) were continuously applied to ten different organic substrate mixes. Retention of the pesticides was similar and in some cases slightly lower in the macrocosms compared to the microcosms. Differences in retention between the different mixes were however minimal. Moreover, the classification of the retention strength of the pesticides was identical to that observed in microcosms: linuron>isoproturon>metalaxyl>bentazone. Monod kinetics were used to describe delayed degradation, which occurred for isoproturon, metalaxyl and bentazone. No breakthrough of linuron was observed, thus, this pesticide was appointed as the most retained and/or degraded pesticide, followed by isoproturon, metalaxyl and bentazone. Finally, most of the matrix mixes efficient in degrading or retaining pesticides were mixes containing dried cow manure.

  14. Characterizing pesticide sorption and degradation in macro scale biopurification systems using column displacement experiments

    Energy Technology Data Exchange (ETDEWEB)

    Wilde, Tineke de [Laboratory of Crop Protection Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium)], E-mail: Tineke.DeWilde@UGent.be; Spanoghe, Pieter [Laboratory of Crop Protection Chemistry, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium); Mertens, Jan; Sniegowksi, Kristel; Ryckeboer, Jaak [Division of Soil and Water Management, Faculty of Bioscience Engineering, K.U. Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee (Belgium); Jaeken, Peter [PCF-Royal Research Station of Gorsem, De Brede Akker 13, 3800 Sint-Truiden (Belgium); Springael, Dirk [Division of Soil and Water Management, Faculty of Bioscience Engineering, K.U. Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee (Belgium)

    2009-04-15

    The efficiency of biopurification systems to treat pesticide-contaminated water was previously studied in microcosms. To validate the obtained results, macrocosm systems were set-up. Four pesticides (linuron, isoproturon, bentazone, and metalaxyl) were continuously applied to ten different organic substrate mixes. Retention of the pesticides was similar and in some cases slightly lower in the macrocosms compared to the microcosms. Differences in retention between the different mixes were however minimal. Moreover, the classification of the retention strength of the pesticides was identical to that observed in microcosms: linuron > isoproturon > metalaxyl > bentazone. Monod kinetics were used to describe delayed degradation, which occurred for isoproturon, metalaxyl and bentazone. No breakthrough of linuron was observed, thus, this pesticide was appointed as the most retained and/or degraded pesticide, followed by isoproturon, metalaxyl and bentazone. Finally, most of the matrix mixes efficient in degrading or retaining pesticides were mixes containing dried cow manure. - Transport of pesticides in macrocosm containing organic substrates.

  15. Micro- and macro-scale self-organization in a dissipative plasma

    Energy Technology Data Exchange (ETDEWEB)

    Skoric, M.M.; Sato, T. [National Inst. for Fusion Science, Toki, Gifu (Japan); Maluckov, A.; Jovanovic, M.S.

    1998-10-01

    We study a nonlinear three-wave interaction in an open dissipative model of stimulated Raman backscattering in a plasma. A hybrid kinetic-fluid scheme is proposed to include anomalous kinetic dissipation due to electron trapping and plasma wave breaking. We simulate a finite plasma with open boundaries and vary a transport parameter to examine a route to spatio-temporal complexity. An interplay between self-organization at micro (kinetic) and macro (wave/fluid) scales is revealed through quasi-periodic and intermittent evolution of dynamical variables, dissipative structures and related entropy rates. An evidence that entropy rate extrema correspond to structural transitions is found. (author)

  16. Negative-resistance voltage-current characteristics of superconductor contact junctions for macro-scale applications

    CERN Document Server

    Takayasu, M; Minervini, J V; 10.1109/TASC.2003.812854

    2003-01-01

    Voltage-current characteristics of mechanical pressure contact junctions between superconducting wires are investigated using a voltage-driving method. It is found that the switching regions at low voltages result from negative resistance of the contact junction. The current transport of the contact junctions is discussed from the perspective of two existing models: the multiple Andreev reflections at the two SN interfaces of a SNS (Superconductor/Normal metal /Superconductor) junction and the inhomogeneous multiple Josephson weak-link array. (13 refs).

  17. Turbulent velocity and concentration measurements in a macro-scale multi-inlet vortex nanoprecipitation reactor

    Science.gov (United States)

    Liu, Zhenping; Fox, Rodney; Hill, James; Olsen, Michael

    2013-11-01

    Flash Nanoprecipitation (FNP) is a technique to produce monodisperse functional nanoparticles. Microscale multi-inlet vortex reactors (MIVR) have been effectively applied to FNP due to their ability to provide rapid mixing and flexibility of inlet flow conditions. A scaled-up MIVR could potentially generate large quantities of functional nanoparticles, giving FNP wider applicability in industry. In the presented research, the turbulent velocity field inside a scaled-up, macroscale MIVR is measured by particle image velocimetry (PIV). Within the reactor, velocity is measured using both two-dimensional and stereoscopic PIV at two Reynolds numbers (3500 and 8750) based on the flow at each inlet. Data have been collected at numerous locations in the inlet channels, the reaction chamber, and the reactor outlet. Mean velocity and Reynolds stresses have been obtained based on 5000 instantaneous velocity realizations at each measurement location. The turbulent mixing process has also been investigated with passive scalar planar laser-induced fluorescence and simultaneous PIV/PLIF. Velocity and concentration results are compared to results from previous experiments in a microscale MIVR. Scaled profiles of turbulent quantities are similar to those previously found in the microscale MIVR.

  18. Experimental study of erosion by suffusion at the micro-macro scale

    Science.gov (United States)

    Nguyen, Cong Doan; Benahmed, Nadia; Philippe, Pierre; Diaz Gonzalez, Elizabeth Victoria

    2017-06-01

    Internal erosion is a complex phenomenon which represents one of the main sources of risk to the safety of earth hydraulic structures such as embankment dams, dikes and levees. Its occurrence may cause instability and failure of these structures with consequences that can be dramatic. Erosion by suffusion corresponds to the process of detachment and transport, under the action of hydraulic flow, of the finest soil particles within the porous media formed mainly of large grains. Its occurrence usually causes change of the initial microstructure and hence a change in the physical, hydraulic and mechanical characteristics of the soil. In this study, we present first an experimental characterization of the erosion mechanism during its occurrence within a granular soil. Particular emphasis was put on the role of hydraulic conditions in triggering of fines migration. Thereafter, we present a preliminary microstructural characterization of the erosion process through direct visualization by optical techniques of particles migration using crushed glass samples as model materials.

  19. A MACRO-SCALE SEMI-DISTRIBUTED HYDROLOGICAL MODEL AND APPLICATION TO THE DATONG RIVER VALLEY

    Institute of Scientific and Technical Information of China (English)

    YANG Zhi-feng; LIU Lu-liu; SHEN Zhen-yao; GORDON G. Huang

    2005-01-01

    A daily distributed hydrological model was developed using routine hydro-meteorological data on the basis of the raster DEM and land cover data.Then the model was used to model daily runoff of the Datong River Valley located in the upper catchment of the Yellow River Basin.The runoff comprises surface flow, subsurface flow and ground water flow.Evapotranspiration comprises canopy evaporation, snow sublimation and soil evapotranspiration.The infiltration to the soil was estimated with improved Green-Ampt model, and the potential evapotranspiration is estimated with Morton CRAE method, which only needs the routine meteorological data.Simulation results and the comparison with semi-distributed SLURP hydrological model show that the structure of the model presented herein is reasonable.

  20. A global fingerprint of macro-scale changes in urban structure from 1999 to 2009

    Science.gov (United States)

    Frolking, Steve; Milliman, Tom; Seto, Karen C.; Friedl, Mark A.

    2013-06-01

    Urban population now exceeds rural population globally, and 60-80% of global energy consumption by households, businesses, transportation, and industry occurs in urban areas. There is growing evidence that built-up infrastructure contributes to carbon emissions inertia, and that investments in infrastructure today have delayed climate cost in the future. Although the United Nations statistics include data on urban population by country and select urban agglomerations, there are no empirical data on built-up infrastructure for a large sample of cities. Here we present the first study to examine changes in the structure of the world’s largest cities from 1999 to 2009. Combining data from two space-borne sensors—backscatter power (PR) from NASA’s SeaWinds microwave scatterometer, and nighttime lights (NL) from NOAA’s defense meteorological satellite program/operational linescan system (DMSP/OLS)—we report large increases in built-up infrastructure stock worldwide and show that cities are expanding both outward and upward. Our results reveal previously undocumented recent and rapid changes in urban areas worldwide that reflect pronounced shifts in the form and structure of cities. Increases in built-up infrastructure are highest in East Asian cities, with Chinese cities rapidly expanding their material infrastructure stock in both height and extent. In contrast, Indian cities are primarily building out and not increasing in verticality. This new dataset will help characterize the structure and form of cities, and ultimately improve our understanding of how cities affect regional-to-global energy use and greenhouse gas emissions.

  1. Solving for Micro- and Macro- Scale Electrostatic Configurations Using the Robin Hood Algorithm

    CERN Document Server

    Formaggio, J A; Corona, T J; Stefancic, H; Abraham, H; Gluck, F

    2011-01-01

    We present a novel technique by which highly-segmented electrostatic configurations can be solved. The Robin Hood method is a matrix-inversion algorithm optimized for solving high density boundary element method (BEM) problems. We illustrate the capabilities of this solver by studying two distinct geometry scales: (a) the electrostatic potential of a large volume beta-detector and (b) the field enhancement present at surface of electrode nano-structures. Geometries with elements numbering in the O(10^5) are easily modeled and solved without loss of accuracy. The technique has recently been expanded so as to include dielectrics and magnetic materials.

  2. Transpression / transtension: a model for micro- to macro-scale deformation

    Science.gov (United States)

    Sanderson, David J.

    2014-05-01

    Transpression and transtension were terms introduces by Harland (1971) to define deformation that involves both transcurrent (strike-slip) movement along a zone and compression or extension across it. Sanderson & Marchini (1984) produced a strain model for transpression, and the concept has subsequently been applied in a variety of tectonic settings over a wide range of scales. Transpression is modelled by the simultaneous application of a transcurrent shear and horizontal shortening orthogonal to a block, with no lateral stretch. Sanderson & Marchini originally used two parameters α (the vertical elongation) and γ (the shear strain on the zone boundary) to define the deformation within the block. For constant volume deformation, the shortening across the zone is simply β = α-1, but volume change (Δ) is easily incorporated in the models, where α β = (1+Δ). One may also specify transpression in terms of the strain rates (δɛsgγ) and the direction (A) and amount (S) of convergence/divergence, where tan A = δγ / δɛ. The transpressional model has a number of important implications, which include: It generally leads to triaxial deformation, hence is intrinsically 3-dimensional, e.g. flattening strains characterise transpressional zones, whereas constrictional strains result from transtension. It represents a spectrum of strain states, providing a useful way of classifying deformational styles between generalised compressional, strike-slip and extensional regimes. The vorticity axis will be normal to the shear direction (vertical) and does not need to be parallel to the intermediate principle stain axis. At a convergence angle of A ≡70.5O the incremental and finite strain axes may be differently oriented and this may produce situations where structures may appear to develop in unusual orientations with respect to the finite strain fabrics Both the compressional and shear components contribute to the stretch Sn normal to the zone, where Sn = (α2 + γ2)-1 /2 . Thus the shortening (stretch) measured across a transpression zone (including simple shear zones), by methods such as balancing sections, should not be confused with the parameter β, which measures the convergence of the zone boundaries. It may be applied to zones of deformation on a variety of scales. It has been generalised to include several other components of deformation, including lateral extrusion, vertical shear both parallel and normal to the zone and oblique shear, inclined shear, etc. The controlling parameters may vary spatially within a zone (spatial partitioning) or with time during its evolution (temporal partitioning). In many settings involving oblique convergence, the strike-slip component may be taken up mainly on faults within the zone, whilst the compression is more broadly distributed. Spatial partitioning can allow continuity across the zone boundary (e.g. Robin & Cruden 1994).

  3. From Micro- to Macro-scales in the Heliosphere and Magnetospheres

    CERN Document Server

    Shaikh, Dastgeer; Lu, Q M; Zank, G P

    2010-01-01

    From a broader perspective, the heliosphere and planetary magnetospheres provide a test bed to explore the plasma physics of the Universe. In particular, the underlying nonlinear coupling of different spatial and temporal scales plays a key role in determining the structure and dynamics of space plasmas and electromagnetic fields. Plasmas and fields exhibit both laminar and turbulent properties, corresponding to either well organized or disordered states, and the development of quantitative theoretical and analytical descriptions from physics based first principles is a profound challenge. Limited observations and complications introduced by geometry and physical parameters conspire to complicate the problem. Dimensionless scaling analysis and statistical methods are universally applied common approaches that allow for the application of related ideas to multiple physical problems. We discuss several examples of the interplay between the scales in a variety of space plasma environments, as exemplified in the ...

  4. Characterizing Micro- and Macro-Scale Seismicity from Bayou Corne, Louisiana

    Science.gov (United States)

    Baig, A. M.; Urbancic, T.; Karimi, S.

    2013-12-01

    The initiation of felt seismicity in Bayou Corne, Louisiana, coupled with other phenomena detected by residents on the nearby housing development, prompted a call to install a broadband seismic network to monitor subsurface deformation. The initial deployment was in place to characterize the deformation contemporaneous with the formation of a sinkhole located in close proximity to a salt dome. Seismic events generated during this period followed a swarm-like behaviour with moment magnitudes culminating around Mw2.5. However, the seismic data recorded during this sequence suffer from poor signal to noise, onsets that are very difficult to pick, and the presence of a significant amount of energy arriving later in the waveforms. Efforts to understand the complexity in these waveforms are ongoing, and involve invoking the complexities inherent in recording in a highly attenuating swamp overlying a complex three-dimensional structure with the strong material property contrast of the salt dome. In order to understand the event character, as well as to locally lower the completeness threshold of the sequence, a downhole array of 15 Hz sensors was deployed in a newly drilled well around the salt dome. Although the deployment lasted a little over a month in duration, over 1000 events were detected down to moment magnitude -Mw3. Waveform quality tended to be excellent, with very distinct P and S wave arrivals observable across the array for most events. The highest magnitude events were seen as well on the surface network and allowed for the opportunity to observe the complexities introduced by the site effects, while overcoming the saturation effects on the higher-frequency downhole geophones. This hybrid downhole and surface array illustrates how a full picture of subsurface deformation is only made possible by combining the high-frequency downhole instrumentation to see the microseismicity complemented with a broadband array to accurately characterize the source parameters for the larger magnitude events. Our presentation is focused on investigating this deformation, characterizing the scaling behaviour and the other source processes by taking advantage of the wide-band afforded to us through the deployment.

  5. Aberrant brain regional homogeneity and functional connectivity in middle-aged T2DM patients: a resting-state functional MRI study

    Directory of Open Access Journals (Sweden)

    Daihong Liu

    2016-09-01

    Full Text Available Type 2 diabetes mellitus (T2DM has been associated with cognitive impairment. However, its neurological mechanism remains elusive. Combining regional homogeneity (ReHo and functional connectivity (FC analyses, the present study aimed to investigate brain functional alterations in middle-aged T2DM patients, which could provide complementary information for the neural substrates underlying T2DM-associated brain dysfunction. Twenty-five T2DM patients and 25 healthy controls were involved in neuropsychological testing and structural and resting-state functional magnetic resonance imaging data acquisition. ReHo analysis was conducted to determine the peak coordinates of brain regions with abnormal local brain activity synchronization. Then, the identified brain regions were considered as seeds, and FC between these brain regions and global voxels was computed. Finally, the potential correlations between the imaging indices and neuropsychological data were also explored. Compared with healthy controls, T2DM patients exhibited higher ReHo values in the anterior cingulate gyrus and lower ReHo in right fusiform gyrus, right precentral gyrus and right medial orbit of the superior frontal gyrus. Considering these areas as seed regions, T2DM patients displayed aberrant FC, mainly in the frontal and parietal lobes. The pattern of FC alterations in T2DM patients was characterized by decreased connectivity and positive to negative or negative to positive converted connectivity. Digital Span Test forward scores revealed significant correlations with the ReHo values of the right precentral gyrus (ρ = 0.527, p = 0.014 and FC between the right fusiform gyrus and middle temporal gyrus (ρ = -0.437, p = 0.048. Our findings suggest that T2DM patients suffer from cognitive dysfunction related to spatially local and remote brain activity synchronization impairment. The patterns of ReHo and FC alterations shed light on the mechanisms underlying T2DM-associated brain

  6. DM在航油管道外防腐层检测中的应用%The Application of DM at the External Anticorrosion Coating Detection of a Fuel Pipeline

    Institute of Scientific and Technical Information of China (English)

    陈智君; 李勇樊; 顾平

    2016-01-01

    本文通过研究各种管道检测技术的基本原理,对比了它们的优缺点,强调了综合检测技术的重要性。在此基础上介绍了DM(Defect Mapper)管道防腐层检测仪的基本原理和使用方法,并在某航油管道外防腐层检测中得到应用,获得良好的检测效果。%This paper introduced the basic principle of different pipeline detection technology and compared their advantages and disadvantages and emphasized the importance of comprehensive testing technology. We also introduced the basic principles and methods of DM pipeline coating detector. And get good results through its practical application in some jet fuel pipeline.

  7. Emerging Technologies: Biosecurity and Consequence Management Implications

    Science.gov (United States)

    Perkins, Dana; Nordmann, Brian

    The natural outbreaks of disease and pandemics are transnational threats that create international challenges when detection and containment are not timely due to scarce human and material resources. Whether the cause of those outbreaks is natural or intentional in origin, the main goal of consequence management operations is to save lives. The consequence management process is a continuum of inter-connected phases such as planning, preparation, response, and recovery. The rapid advances of life sciences and the emergence of dual-use technologies such as synthetic biology and nanotechnology pose additional challenges in terms of planning for the unknown potential threats whether they may be synthetic microorganisms with unpredictable dissemination patterns or nanoscale-manipulated biological agents evading current detection capabilities. The US National Strategy for Countering Biological Threats is emphasizing prevention while continuing to support the national preparedness goals and response/recovery capabilities. The recent policies, guidelines, and recommendations on overhauling the biological risk management in the United States are a proactive stance to a rapidly changing global environment. They include optimization of the current oversight frameworks and active engagement of the industry and academia in order to reduce the risk that individuals with ill intent may exploit the commercial application of nucleic acid synthesis technology to access genetic material derived from or by encoding Biological Select Agents or Toxins. We are also actively seeking to increase our knowledge of health effects of various types of nanomaterials, and how to assess, control, and prevent harmful exposure, taking into consideration the numerous gaps that currently exist with regard to the distinct behavior of nanoparticles compared to the same chemical or material at "macro-scale". Fundamentally, a biological incident, whether it is of natural, accidental, or deliberate origin

  8. [THE ROLE OF sbr/Dm nxf1 GENE DURING SYNCYTIAL PERIODS OF DEVELOPMENT IN DROSOPHILA MELANOGASTER].

    Science.gov (United States)

    Golubkova, E V; Atsapkina, A A; Mamon, L A

    2015-01-01

    The syncytial development is a feature of early embryogenesis and spermatogenesis in Drosophila melanogaster. All elements of syncytium are interconnected by single cytoskeletal network that enables equal conditions and provides synchronic development. The cytoskeleton is essential for the formation and functioning of the mitotic spindle, cytoskeletal elements are the main structural component of cilia and flagella. Intra- and intercellular transport, morphogenesis processes depend from cytoskeleton on both within a single cell, and at the level of the whole organism. The sbr (small bristles) gene of D. melanogaster belongs to the NXF (nuclear export factor) evolutionarily conservative proteins family. Gene Dm nxf1 (sbr), as well as its orthologs in other organisms, controls the export of poly(A)-containing RNA from the nucleus to the cytoplasm, and the corresponding proteins are usually localized in the nucleus or in the nuclear envelope. For SBR protein we have shown the localization not only in the nucleus, but in the cytoplasm marking of characteristic cytoplasmic structures. A breach of the cytoskeleton in the sbr (Dm nxf1) mutant in D. melanogaster shown by us and cytoplasmic localization of the protein SBR allow us to link the specific functions of this protein with the dynamics of the cytoskeleton.

  9. Erectile Dysfunction and Low Sex Drive in Men with Type 2 DM: The Potential Role of Diabetic Pharmacotherapy

    Science.gov (United States)

    Al-Gareeb, Ali I.

    2016-01-01

    Introduction Diabetic men with erectile dysfunction have not been widely studied. They have low testosterone levels, causing low sex drive and erectile dysfunction. Aim To assess the erectile dysfunction and sex drive in relation to testosterone serum levels in type 2 Diabetes Mellitus (DM) patients. Materials and Methods A total of 64 patients with type 2 DM were enrolled in this cross-sectional study, according to the treatment types they were divided into three groups, group (A): 34 patients treated with metformin, group (B): 30 patients treated with sulfonylurea and group (C): 27 healthy normal non-diabetic men are taken as control. Total testosterone (TT), Free Testosterone (FT), Free Androgenic Index (FAI), Sex Hormone Binding Globulin (SHBG), lipid profile and anthropometric parameters in metformin and sulfonylurea treated patients were compared to normal healthy men along with Sexual Health Inventory for Men (SHIM). Results Total testosterone serum levels were high in sulfonylurea treated patients as compared to metformin treated patients’ p elevation in testosterone levels, sex drive and erectile function. PMID:28208875

  10. Research of CBR, DM and smart algorithms based design methods for high-rise building structure form-selection

    Institute of Scientific and Technical Information of China (English)

    ZHANG Shi-hai; LIU Shu-jun; LIU Xiao-yan; OU Jin-ping

    2006-01-01

    First, the high-rise building structure design process is divided into three relevant steps, that is,scheme generation and creation, performance evaluation, and scheme optimization. Then with the application of relational database, the case database of high-rise structures is constructed, the structure form-selection designing methods such as the smart algorithm based on CBR, DM, FINS, NN and GA is presented, and the original forms system of this method and its general structure are given. CBR and DM are used to generate scheme candidates; FINS and NN to evaluate and optimize the scheme performance; GA to create new structure forms.Finally, the application cases are presented, whose results fit in with the real project. It proves by combining and using the expert intelligence, algorithm intelligence and machine intelligence that this method makes good use of not only the engineering project knowledge and expertise but also much deeper knowledge contained in various engineering cases. In other words, it is because the form selection has a strong background support of vast real cases that its results prove more reliable and more acceptable. So the introduction of this method prorides an effective approach to improving the quality, efficiency, automatic and smart level of high-rise structures form selection design.

  11. Comissioning of TREX-DM, a low background Micromegas-based Time Projection Chamber for low mass WIMP detection

    CERN Document Server

    Iguaz, F J; Aznar, F; Castel, J F; Cebrian, S; Dafni, T; Garcia, J A; Irastorza, I G; Lagraba, A; Luzon, G; Peiro, A

    2015-01-01

    Dark Matter experiments are recently focusing their detection techniques in low-mass WIMPs, which requires the use of light elements and low energy threshold. In this context, we describe the TREX-DM experiment, a low background Micromegas-based Time Projection Chamber for low-mass WIMP detection. Its main goal is the operation of an active detection mass $\\sim$0.3 kg, with an energy threshold below 0.4~keVee and fully built with previously selected radiopure materials. This work focuses on the commissioning of the actual setup situated in a laboratory on surface. A preliminary background model of the experiment is also presented, based on Geant4 simulations and two discrimination methods: a conservative muon/electron and one based on a $^{252}$Cf source. Based on this model, TREX-DM could be competitive in the search for low mass WIMPs and, in particular, it could be sensitive to the WIMP interpretation of the DAMA/LIBRA hint.

  12. Contrasting Galaxy Formation from Quantum Wave Dark Matter, $\\psi$DM, with $\\Lambda$CDM, using Planck and Hubble Data

    CERN Document Server

    Schive, Hsi-Yu; Broadhurst, Tom; Huang, Kuan-Wei

    2015-01-01

    The newly established luminosity functions of high-z galaxies at $4 \\lesssim z \\lesssim 10$ can provide a stringent check on dark matter models that aim to explain the core properties of dwarf galaxies. The cores of dwarf spheroidal galaxies are understood to be too large to be accounted for by free streaming of warm dark matter without overly suppressing the formation of such galaxies. Here we demonstrate with cosmological simulations that wave dark matter, $\\psi$DM, appropriate for light bosons such as axions, does not suffer this problem, given a boson mass of $m_{\\psi} \\ge 1.2 \\times 10^{-22}{\\,\\rm eV}$ ($2\\sigma$). In this case, the halo mass function is suppressed below $\\sim 10^{10}{\\,M_\\odot}$ at a level that is consistent with the high-z luminosity functions, while simultaneously generating the kpc-scale cores in dwarf galaxies arising from the solitonic ground state in $\\psi$DM. We demonstrate that the reionization history in this scenario is consistent with the Thomson optical depth recently report...

  13. Transport of platinum bonded nucleotides into proteoliposomes, mediated by Drosophila melanogaster thiamine pyrophosphate carrier protein (DmTpc1).

    Science.gov (United States)

    Carrisi, Chiara; Antonucci, Daniela; Lunetti, Paola; Migoni, Danilo; Girelli, Chiara R; Dolce, Vincenza; Fanizzi, Francesco P; Benedetti, Michele; Capobianco, Loredana

    2014-01-01

    The results of the present study suggest that DmTpc1 is actively implicated in the specific uptake of free cytoplasmic Pt bonded nucleotides, and therefore could be linked to the mechanism of action of some platinum-based antitumor drugs. Although DmTpc1 has a low affinity for model [Pt(dien)(N7-5'-dGTP)] and cis-[Pt(NH3)2(py)(N7-5'-dGTP)] compared to dATP it's well known that DNA platination level of few metal atoms per double-stranded molecule may account for the pharmacological activity of platinum based antitumor drugs. This is the first investigation where it has been demonstrated that a mitochondrial carrier is directly involved in the transport of metalated purines related with the cisplatin mechanism of action. Moreover it is shown as a lower hindrance of nucleotide bonded platinum complexes could strongly enhance mitochondrial uptake. Furthermore, a new application of ICP-AES addressed to measure the transport of metalated nucleobases, by using a recombinant protein reconstituted into liposomes, has been here, for the first time, developed and compared with a standard technique such as the liquid scintillation counting.

  14. 基于TMS320DM642的矿用数字化扩音电话系统设计%Design of Digital Broadcast Telephone System of Coal Mine Based on TMS320DM642

    Institute of Scientific and Technical Information of China (English)

    马正华; 姚刘君; 周炯如

    2012-01-01

    This paper analyzes the existing drawbackg of broadcast telephone system of coal mine, and introduces a design scheme of digital broadcast telephone system of coal mine baaed on Texas Instruments (TI) TMS320DM642 digital processor and TCP/IP protocol. The system makes use of audio codec chip TLV320AIC23B to achieve audio codec and adaptive anti-noise algorithm so that the output audio' s quality could be good to meet standard of the voice in the mine, and then takes advantage of seamlessly connectivity between TMS32ODM642 EMAC and physical layer chip Intel LXT971 to complete digital voice transmission based on embedded TCP/IP protocol stack. In the System software portion, the system integrates audio collection task, audio processing task, network transmission and other tasks by RF5 in the DSP/BIOS. In the telephone terminal voice signals can be output through speakers or headphones to achieve high-quality, remote, and stable voice communications under mine.%通过分析目前的矿用扩音电话系统的现状,为解决矿井下语音通信中高噪声强干扰问题,提出一种基于数字处理器TMS320DM642和嵌入式TCP/IP协议的矿用数字化扩音电话系统的设计方案;采用音频编解码芯片TLV320AIC23B实现语音处理以达到矿井下语音质量的要求,并利用物理层芯片Intel LXT971与TMS320DM642的无缝连接,实现基于嵌入式TCP/IP协议栈的数字语音传输;系统软件采用RF5框架开发,基于DSP/BIOS实现语音采集,语音处理,网络传输等任务;通过对各关键模块测试与分析,系统运行稳定可靠,实现矿用数字化扩音电话的高质量,远距离和稳定的语音通信.

  15. Pengendalian status gizi, kadar glukosa darah, dan tekanan darah melalui terapi gizi medis pada pasien diabetes mellitus (DM tipe 2 rawat jalan di RSU Mataram NTB

    Directory of Open Access Journals (Sweden)

    Suhaema Suhaema

    2010-11-01

    Conclusion: Intake of nutrient, nutrition status, blood glucose level and blood pressure of type 2 DM patients that got MNT was more controllable than of those that got conventional nutrition counseling.

  16. The Dionaea muscipula ammonium channel DmAMT1 provides NH₄⁺ uptake associated with Venus flytrap's prey digestion.

    Science.gov (United States)

    Scherzer, Sönke; Krol, Elzbieta; Kreuzer, Ines; Kruse, Jörg; Karl, Franziska; von Rüden, Martin; Escalante-Perez, Maria; Müller, Thomas; Rennenberg, Heinz; Al-Rasheid, Khaled A S; Neher, Erwin; Hedrich, Rainer

    2013-09-09

    Ammonium transporter (AMT/MEP/Rh) superfamily members mediate ammonium uptake and retrieval. This pivotal transport system is conserved among all living organisms. For plants, nitrogen represents a macronutrient available in the soil as ammonium, nitrate, and organic nitrogen compounds. Plants living on extremely nutrient-poor soils have developed a number of adaptation mechanisms, including a carnivorous lifestyle. This study addresses the molecular nature, function, and regulation of prey-derived ammonium uptake in the Venus flytrap, Dionaea muscipula, one of the fastest active carnivores. The Dionaea muscipula ammonium transporter DmAMT1 was localized in gland complexes where its expression was upregulated upon secretion. These clusters of cells decorating the inner trap surface are engaged in (1) secretion of an acidic digestive enzyme cocktail and (2) uptake of prey-derived nutrients. Voltage clamp of Xenopus oocytes expressing DmAMT1 and membrane potential recordings with DmAMT1-expressing Dionaea glands were used to monitor and compare electrophysiological properties of DmAMT1 in vitro and in planta. DmAMT1 exhibited the hallmark biophysical properties of a NH4(+)-selective channel. At depolarized membrane potentials (Vm = 0), the Km (3.2 ± 0.3 mM) indicated a low affinity of DmAMT1 for ammonium that increased systematically with negative going voltages. Upon hyperpolarization to, e.g., -200 mV, a Km of 0.14 ± 0.015 mM documents the voltage-dependent shift of DmAMT1 into a NH4(+) transport system of high affinity. We suggest that regulation of glandular DmAMT1 and membrane potential readjustments of the endocrine cells provide for effective adaptation to varying, prey-derived ammonium sources. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Presence of elevated non-HDL among patients with T2DM with CV events despite of optimal LDL-C - A report from South India.

    Science.gov (United States)

    Kumpatla, Satyavani; Soni, Anju; Narasingan, S N; Viswanathan, Vijay

    2016-01-01

    Elevated non-high density lipoprotein cholesterol (non-HDL-C) was the commonest lipid abnormality among T2DM patients with cardiovascular events (CV) events. Prevalence of elevated non-HDL-C was 21.6% among patients who were on statin therapy and with optimal low density lipoprotein-cholesterol (LDL-C) levels. Despite an optimal LDL-C level, 47% of the T2DM patients with CV events had elevated non-HDL-C.

  18. Molecular, physiological, and motor performance defects in DMSXL mice carrying >1,000 CTG repeats from the human DM1 locus.

    Directory of Open Access Journals (Sweden)

    Aline Huguet

    Full Text Available Myotonic dystrophy type 1 (DM1 is caused by an unstable CTG repeat expansion in the 3'UTR of the DM protein kinase (DMPK gene. DMPK transcripts carrying CUG expansions form nuclear foci and affect splicing regulation of various RNA transcripts. Furthermore, bidirectional transcription over the DMPK gene and non-conventional RNA translation of repeated transcripts have been described in DM1. It is clear now that this disease may involve multiple pathogenic pathways including changes in gene expression, RNA stability and splicing regulation, protein translation, and micro-RNA metabolism. We previously generated transgenic mice with 45-kb of the DM1 locus and >300 CTG repeats (DM300 mice. After successive breeding and a high level of CTG repeat instability, we obtained transgenic mice carrying >1,000 CTG (DMSXL mice. Here we described for the first time the expression pattern of the DMPK sense transcripts in DMSXL and human tissues. Interestingly, we also demonstrate that DMPK antisense transcripts are expressed in various DMSXL and human tissues, and that both sense and antisense transcripts accumulate in independent nuclear foci that do not co-localize together. Molecular features of DM1-associated RNA toxicity in DMSXL mice (such as foci accumulation and mild missplicing, were associated with high mortality, growth retardation, and muscle defects (abnormal histopathology, reduced muscle strength, and lower motor performances. We have found that lower levels of IGFBP-3 may contribute to DMSXL growth retardation, while increased proteasome activity may affect muscle function. These data demonstrate that the human DM1 locus carrying very large expansions induced a variety of molecular and physiological defects in transgenic mice, reflecting DM1 to a certain extent. As a result, DMSXL mice provide an animal tool to decipher various aspects of the disease mechanisms. In addition, these mice can be used to test the preclinical impact of systemic

  19. Staufen1 Regulates Multiple Alternative Splicing Events either Positively or Negatively in DM1 Indicating Its Role as a Disease Modifier.

    Directory of Open Access Journals (Sweden)

    Emma Bondy-Chorney

    2016-01-01

    Full Text Available Myotonic dystrophy type 1 (DM1 is a neuromuscular disorder caused by an expansion of CUG repeats in the 3' UTR of the DMPK gene. The CUG repeats form aggregates of mutant mRNA, which cause misregulation and/or sequestration of RNA-binding proteins, causing aberrant alternative splicing in cells. Previously, we showed that the multi-functional RNA-binding protein Staufen1 (Stau1 was increased in skeletal muscle of DM1 mouse models and patients. We also showed that Stau1 rescues the alternative splicing profile of pre-mRNAs, e.g. the INSR and CLC1, known to be aberrantly spliced in DM1. In order to explore further the potential of Stau1 as a therapeutic target for DM1, we first investigated the mechanism by which Stau1 regulates pre-mRNA alternative splicing. We report here that Stau1 regulates the alternative splicing of exon 11 of the human INSR via binding to Alu elements located in intron 10. Additionally, using a high-throughput RT-PCR screen, we have identified numerous Stau1-regulated alternative splicing events in both WT and DM1 myoblasts. A number of these aberrant ASEs in DM1, including INSR exon 11, are rescued by overexpression of Stau1. However, we find other ASEs in DM1 cells, where overexpression of Stau1 shifts the splicing patterns away from WT conditions. Moreover, we uncovered that Stau1-regulated ASEs harbour Alu elements in intronic regions flanking the alternative exon more than non-Stau1 targets. Taken together, these data highlight the broad impact of Stau1 as a splicing regulator and suggest that Stau1 may act as a disease modifier in DM1.

  20. Ischemia‐reperfusion injury and hypoglycemia risk in insulin‐treated T1DM rats following different modalities of regular exercise

    OpenAIRE

    Matthew W. McDonald; Hall, Katharine E.; Jiang, Mao; Noble, Earl G.; Melling, C.W. James

    2014-01-01

    Abstract While regular exercise is known to improve cardiovascular function, individuals with type 1 diabetes mellitus (T1DM) have an increased risk for exercise‐induced hypoglycemia. Clinical data suggest that higher intensities of acute exercise may alleviate the onset of hypoglycemia; however, the cardiovascular benefit from these forms of exercise in patients with T1DM has yet to be established. The purpose of this study was to investigate the cardiovascular benefit of different regular e...

  1. Arsenic exposure and calpain-10 polymorphisms impair the function of pancreatic beta-cells in humans: a pilot study of risk factors for T2DM.

    Directory of Open Access Journals (Sweden)

    Andrea Díaz-Villaseñor

    Full Text Available The incidence of type 2 diabetes mellitus (T2DM is increasing worldwide and diverse environmental and genetic risk factors are well recognized. Single nucleotide polymorphisms (SNPs in the calpain-10 gene (CAPN-10, which encodes a protein involved in the secretion and action of insulin, and chronic exposure to inorganic arsenic (iAs through drinking water have been independently associated with an increase in the risk for T2DM. In the present work we evaluated if CAPN-10 SNPs and iAs exposure jointly contribute to the outcome of T2DM. Insulin secretion (beta-cell function and insulin sensitivity were evaluated indirectly through validated indexes (HOMA2 in subjects with and without T2DM who have been exposed to a gradient of iAs in their drinking water in northern Mexico. The results were analyzed taking into account the presence of the risk factor SNPs SNP-43 and -44 in CAPN-10. Subjects with T2DM had significantly lower beta-cell function and insulin sensitivity. An inverse association was found between beta-cell function and iAs exposure, the association being more pronounced in subjects with T2DM. Subjects without T2DM who were carriers of the at-risk genotype SNP-43 or -44, also had significantly lower beta-cell function. The association of SNP-43 with beta-cell function was dependent on iAs exposure, age, gender and BMI, whereas the association with SNP-44 was independent of all of these factors. Chronic exposure to iAs seems to be a risk factor for T2DM in humans through the reduction of beta-cell function, with an enhanced effect seen in the presence of the at-risk genotype of SNP-43 in CAPN-10. Carriers of CAPN-10 SNP-44 have also shown reduced beta-cell function.

  2. Arsenic Exposure and Calpain-10 Polymorphisms Impair the Function of Pancreatic Beta-Cells in Humans: A Pilot Study of Risk Factors for T2DM

    Science.gov (United States)

    Díaz-Villaseñor, Andrea; Cruz, Laura; Cebrián, Arturo; Hernández-Ramírez, Raúl U.; Hiriart, Marcia; García-Vargas, Gonzálo; Bassol, Susana; Sordo, Monserrat; Gandolfi, A. Jay; Klimecki, Walter T.; López-Carillo, Lizbeth; Cebrián, Mariano E.; Ostrosky-Wegman, Patricia

    2013-01-01

    The incidence of type 2 diabetes mellitus (T2DM) is increasing worldwide and diverse environmental and genetic risk factors are well recognized. Single nucleotide polymorphisms (SNPs) in the calpain-10 gene (CAPN-10), which encodes a protein involved in the secretion and action of insulin, and chronic exposure to inorganic arsenic (iAs) through drinking water have been independently associated with an increase in the risk for T2DM. In the present work we evaluated if CAPN-10 SNPs and iAs exposure jointly contribute to the outcome of T2DM. Insulin secretion (beta-cell function) and insulin sensitivity were evaluated indirectly through validated indexes (HOMA2) in subjects with and without T2DM who have been exposed to a gradient of iAs in their drinking water in northern Mexico. The results were analyzed taking into account the presence of the risk factor SNPs SNP-43 and -44 in CAPN-10. Subjects with T2DM had significantly lower beta-cell function and insulin sensitivity. An inverse association was found between beta-cell function and iAs exposure, the association being more pronounced in subjects with T2DM. Subjects without T2DM who were carriers of the at-risk genotype SNP-43 or -44, also had significantly lower beta-cell function. The association of SNP-43 with beta-cell function was dependent on iAs exposure, age, gender and BMI, whereas the association with SNP-44 was independent of all of these factors. Chronic exposure to iAs seems to be a risk factor for T2DM in humans through the reduction of beta-cell function, with an enhanced effect seen in the presence of the at-risk genotype of SNP-43 in CAPN-10. Carriers of CAPN-10 SNP-44 have also shown reduced beta-cell function. PMID:23349674

  3. Diabetes and pulmonary tuberculosis: a global overview with special focus on the situation in Asian countries with high TB-DM burden

    Science.gov (United States)

    Zheng, Chunlan; Hu, Minhui; Gao, Feng

    2017-01-01

    ABSTRACT Background: The double burden of tuberculosis (TB) and diabetes mellitus (DM) is hitting certain Asian countries harder than other areas. In a global estimate, 15% of all TB cases could be attributable to DM, with 40% of those cases coming from India and China. Many other countries of South, East, and South-East Asia are of particular concern given their TB burdens, large projected increases in DM prevalence, and population size. Objective: In this narrative review, we aimed to: (i) give an overall insight into the evidence on TB-DM epidemiology from high double burden Asian countries, (ii) present the evidence on bi-directional screening implementation in this region, (iii) discuss possible factors related to higher TB susceptibility of Asian diabetic patients, and (iv) identify TB-DM comorbidity treatment challenges. Methods: The PubMed and Google Scholar databases were searched for all studies addressing DM/TB epidemiology, bi-directional screening and management in South, East and South-East Asia. Results: We identified the DM prevalences among TB patients as ranging from approximately 5% to more than 50%, whereas TB prevalences among diabetic patients were 1.8–9.5 times higher than in the general population in developing Asian countries. Evidence from studies designed to address diagnosis and treatment of the dual disease in these critical regions is scarce as well as the evidence related to possible DM patients’ genetic and acquired predisposition for TB. Conclusion: More prospective studies specifically designed to address adequate screening techniques, identify patients at risk, and define an adequate treatment of dual disease in this region are needed without delay. PMID:28245710

  4. Molecular, physiological, and motor performance defects in DMSXL mice carrying >1,000 CTG repeats from the human DM1 locus.

    Science.gov (United States)

    Huguet, Aline; Medja, Fadia; Nicole, Annie; Vignaud, Alban; Guiraud-Dogan, Céline; Ferry, Arnaud; Decostre, Valérie; Hogrel, Jean-Yves; Metzger, Friedrich; Hoeflich, Andreas; Baraibar, Martin; Gomes-Pereira, Mário; Puymirat, Jack; Bassez, Guillaume; Furling, Denis; Munnich, Arnold; Gourdon, Geneviève

    2012-01-01

    Myotonic dystrophy type 1 (DM1) is caused by an unstable CTG repeat expansion in the 3'UTR of the DM protein kinase (DMPK) gene. DMPK transcripts carrying CUG expansions form nuclear foci and affect splicing regulation of various RNA transcripts. Furthermore, bidirectional transcription over the DMPK gene and non-conventional RNA translation of repeated transcripts have been described in DM1. It is clear now that this disease may involve multiple pathogenic pathways including changes in gene expression, RNA stability and splicing regulation, protein translation, and micro-RNA metabolism. We previously generated transgenic mice with 45-kb of the DM1 locus and >300 CTG repeats (DM300 mice). After successive breeding and a high level of CTG repeat instability, we obtained transgenic mice carrying >1,000 CTG (DMSXL mice). Here we described for the first time the expression pattern of the DMPK sense transcripts in DMSXL and human tissues. Interestingly, we also demonstrate that DMPK antisense transcripts are expressed in various DMSXL and human tissues, and that both sense and antisense transcripts accumulate in independent nuclear foci that do not co-localize together. Molecular features of DM1-associated RNA toxicity in DMSXL mice (such as foci accumulation and mild missplicing), were associated with high mortality, growth retardation, and muscle defects (abnormal histopathology, reduced muscle strength, and lower motor performances). We have found that lower levels of IGFBP-3 may contribute to DMSXL growth retardation, while increased proteasome activity may affect muscle function. These data demonstrate that the human DM1 locus carrying very large expansions induced a variety of molecular and physiological defects in transgenic mice, reflecting DM1 to a certain extent. As a result, DMSXL mice provide an animal tool to decipher various aspects of the disease mechanisms. In addition, these mice can be used to test the preclinical impact of systemic therapeutic

  5. “I Know that You Know that I Know”: Neural Substrates Associated with Social Cognition Deficits in DM1 Patients

    Science.gov (United States)

    Serra, Laura; Cercignani, Mara; Bruschini, Michela; Cipolotti, Lisa; Mancini, Matteo; Silvestri, Gabriella; Petrucci, Antonio; Bucci, Elisabetta; Antonini, Giovanni; Licchelli, Loretta; Spanò, Barbara; Giacanelli, Manlio; Caltagirone, Carlo; Meola, Giovanni; Bozzali, Marco

    2016-01-01

    Myotonic dystrophy type-1 (DM1) is a genetic multi-systemic disorder involving several organs including the brain. Despite the heterogeneity of this condition, some patients with non-congenital DM1 can present with minimal cognitive impairment on formal testing but with severe difficulties in daily-living activities including social interactions. One explanation for this paradoxical mismatch can be found in patients’ dysfunctional social cognition, which can be assessed in the framework of the Theory of Mind (ToM). We hypothesize here that specific disease driven abnormalities in DM1 brains may result in ToM impairments. We recruited 20 DM1 patients who underwent the “Reading the Mind in the Eyes” and the ToM-story tests. These patients, together with 18 healthy controls, also underwent resting-state functional MRI. A composite Theory of Mind score was computed for all recruited patients and correlated with their brain functional connectivity. This analysis provided the patients’ “Theory of Mind-network”, which was compared, for its topological properties, with that of healthy controls. We found that DM1 patients showed deficits in both tests assessing ToM. These deficits were associated with specific patterns of abnormal connectivity between the left inferior temporal and fronto-cerebellar nodes in DM1 brains. The results confirm the previous suggestions of ToM dysfunctions in patients with DM1 and support the hypothesis that difficulties in social interactions and personal relationships are a direct consequence of brain abnormalities, and not a reaction symptom. This is relevant not only for a better pathophysiological comprehension of DM1, but also for non-pharmacological interventions to improve clinical aspects and impact on patients’ success in life. PMID:27258100

  6. An embedded intelligent directed lessons recording system based on TI DM365%基于TI DM365的嵌入式智能导播录课系统

    Institute of Scientific and Technical Information of China (English)

    孙小期; 王晓东; 汤锐彬; 张婷; 刘家璇; 陈芬

    2015-01-01

    In recent years, with the launch of MOOC, Flipped Classroom and other online education products, the Internet is gradually changing the traditional way of teaching. The key factor of those online education products is recording quality , individualized teaching videos. This paper describes an embedded intelligent directed lessons recording system based on TI DM365 , which uses TI DM365 DSP chip receiving three-way video and two-way audio on hardware and provides USB , LAN and WiFi interfaces. Using FFmpeg open source library of advanced audio and video codec library libavcodec , the PC software of this system is developed by VS2013 C ++. The system has a multi-channel audio and video francis decoding, six kinds of intelligence directed and switch modes, local, cloud storage and on-demand playback functions. System test results show that the system performance is stable, the delay of audio and video is less than 100 ms and the recorded video resolution reaches 1080 p, which suggest sound quality is good. Compared with the existing lessons record mode, the system has a series of advantages such as simplicity, convenience, economization and humanization.%近年来,随着 MOOC、翻转课堂等在线教育产品的推出,互联网正渐渐改变着传统的教学方式,其关键因素在于优质、个性化教学视频的录制。本文介绍了一种基于 TI DM365芯片的嵌入式智能导播录课系统,硬件上采用 TI DM365 DSP 芯片接收3路视频和2路音频,并提供 USB、LAN及 WiFi 接口;软件上采用 FFmpeg 开源库中先进的音视频编解码库 libavcodec ,系统上位机软件基于VS2013 C++开发。该系统具有多路音、视频混流解码,六种智能导播和切换模式,本地、云端存储和点播回放等功能。系统测试结果表明:该系统性能稳定、音视频延时率小于100 ms ,所录制的视频的分辨率达到1080 p、音质效果佳。与现有的录课方式相比,本系统具有简

  7. RESEARCH OF ALGORITHMS FOR DM3 MULTI-SOURCE AREHOUSE CONSISTENCY%DM3多数据源数据仓库的一致性维护算法研究

    Institute of Scientific and Technical Information of China (English)

    王元珍; 熊己兴

    2001-01-01

    数据仓库是一个集成了多个分布式、自治或异构数据源上的信息的数据储藏室,以支持用户的查询和分析.本文介绍了DM3数据仓库实现多数据源实化视图一致性维护的策略,分析了产生视图不一致性的原因和解决办法,以及改进后的一致性维护算法:Strobe*算法和T-Strobe*算法.

  8. Design and implementation of panorama multi-target detection system based on DM642%基干DM642的全景多目标检测系统的设计与实现

    Institute of Scientific and Technical Information of China (English)

    刘歆浏; 田瑞娟; 蒋飞; 周建平

    2014-01-01

    To achieve omni directional monitoring and intelligent warning,a DM642-based panorama multi-target detection system was designed. The construction,achievement and optimization of the system and algorithm are analyzed in the aspects of hardware design and software design. A detail analysis of the system’s operation flaw is done in allusion to complex functions and architecture. The framework is built by DSP/BIOS. The background modeling based on energy accumulation and critical image processing algorithm based on SIFT are applied. Finally,the feasibility was verified by the system debugging.%为了实现全方位监控和智能预警,设计了一套以DM642为核心的全景多目标检测系统。从硬件设计和软件设计两个方面分析了系统和算法的构建、实现和优化。针对该系统复杂的功能和架构,详细分析其工作流程,采用了基于DSP/BIOS的框架搭建,并且设计和采用基于能量累计的背景建模和基于SIFT的图像匹配等关键图像处理算法。并最终通过系统调试验证其设计的可行性。

  9. 基于Davinei-DM6467的高斯混合模型算法的实现%Realization of gaussian mixture model algorithm based on Davinci-DM6467

    Institute of Scientific and Technical Information of China (English)

    刘德方; 王戴木; 邓明; 陈静; 赵正平

    2012-01-01

    针对智能监控中运动目标检测的问题,提出了基于Davinci—DM6467的高斯混合模型像素级的背景分割策略。对彩色图像建立高斯混合模型,根据场景中象素点的稳定性来调整模型参数的更新速率;通过和马氏阈值进行对比来判断是不是要更新背景模型;通过和背景阈值进行对比来判断哪几个模型是属于背景区域。经验证性实验测试,结果表明,高斯混合模型在运动检测中实时性好,对环境有较强的鲁棒性。%In the light of movement target detection during intelligence monitoring, the author put forward Gaussian mixture model of pixel level background segmentation strategy based on Davinei-DM6467. Firstly establishing Gaussian mixture model for colorful images and then adjusting the updating velocity of model parameters according to the stability of each pixels in frames ; secondly comparing them with Markov threshold to judge whether to update the background model; finally comparing them with back-ground threshold to judge which several models belong to background region. Experimental results show that Gaussian mixture model in motion detection is possessed of good real-time and strong robustness to environment.

  10. Evidences of +896 A/G TLR4 Polymorphism as an Indicative of Prevalence of Complications in T2DM Patients

    Directory of Open Access Journals (Sweden)

    Carmela Rita Balistreri

    2014-01-01

    Full Text Available T2DM is today considered as world-wide health problem, with complications responsible of an enhanced mortality and morbidity. Thus, new strategies for its prevention and therapy are necessary. For this reason, the research interest has focused its attention on TLR4 and its polymorphisms, particularly the rs4986790. However, no conclusive findings have been reported until now about the role of this polymorphism in development of T2DM and its complications, even if a recent meta-analysis showed its T2DM association in Caucasians. In this study, we sought to evaluate the weight of rs4986790 polymorphism in the risk of the major T2DM complications, including 367 T2DM patients complicated for the 55.6%. Patients with A/A and A/G TLR4 genotypes showed significant differences in complication’s prevalence. In particular, AG carriers had higher risk prevalence for neuropathy (P=0.026, lower limb arteriopathy (P=0.013, and the major cardiovascular pathologies (P=0.017. Their cumulative risk was significant (P=0.01, with a threefold risk to develop neuropathy, lower limb arteriopathy, and major cardiovascular events in AG cases compared to AA cases. The adjusted OR for the confounding variables was 3.788 (95% CI: 1.642–8.741. Thus, the rs4986790 polymorphism may be an indicative of prevalence of complications in T2DM patients.

  11. Exposure-response relationship of T-DM1: insight into dose optimization for patients with HER2-positive metastatic breast cancer.

    Science.gov (United States)

    Wang, J; Song, P; Schrieber, S; Liu, Q; Xu, Q; Blumenthal, G; Amiri Kordestani, L; Cortazar, P; Ibrahim, A; Justice, R; Wang, Y; Tang, S; Booth, B; Mehrotra, N; Rahman, A

    2014-05-01

    Exposure-response (E-R) analyses for ado-trastuzumab emtansine (T-DM1, Kadcyla) were performed using data from a randomized, active control (lapatinib plus capecitabine) trial in patients with human epidermal growth factor 2-positive metastatic breast cancer. Kaplan-Meier survival analyses stratified by T-DM1 trough concentration on day 21 of cycle 1 (Cmin,C1D21) were performed for overall survival (OS) and progression-free survival (PFS). E-R analyses indicated that after adjusting for baseline risk factors, higher T-DM1 exposure is associated with improved efficacy. T-DM1-treated patients with Cmin,C1D21 lower than the median value had values of OS and PFS comparable to those of the active control arm. The percentage of patients who received T-DM1 dose adjustments was similar across the exposure range and was lower than that of the active control arm. Our findings suggest that there may be an opportunity to optimize Kadcyla dose in the patient subgroup with low T-DM1 exposure for improved efficacy with acceptable tolerability.

  12. CATALYST TECHNOLOGY DEVELOPMENT FROM MACRO-,MICRO- DOWN TO NANO-SCALE

    Institute of Scientific and Technical Information of China (English)

    Wei Liu

    2005-01-01

    Catalyst and catalytic process technology has been an ever-growing field that involves chemical engineering, chemistry, and material science. A number of excellent review articles and books have been published on the subject. In this work, the author reviews the evolution and development of catalyst products with multi-scale methodology.The catalyst technologies are classified into three levels, macro-scale (reactor size), mini- and micro-scale (catalyst unit),and nano-scale (catalyst intrinsic structures). Innovation at different scales requires different sets of expertise, method,and knowledge. Specific examples of significant impact to practical application are used to illustrate technology development at each scale. The multi-scale analysis enables clear delineation of technology components and their relationship for a catalyst product and catalytic process. Manipulation of catalyst structures at nano-scale to increase intrinsic activity and/or selectivity is considered of large potential for future catalyst product development. Recent research results on Cu-CeO2 and Au-CeO2 composite catalysts for air pollution control and hydrogen production are used to show how novel catalytic properties can be discovered by unique combination of different but common materials at the nano-scale.

  13. GPR119 agonists: a promising approach for T2DM treatment? A SWOT analysis of GPR119.

    Science.gov (United States)

    Kang, Sang-Uk

    2013-12-01

    Ever since its advent as a promising therapeutic target for type 2 diabetes mellitus (T2DM), G-protein-coupled receptor 119 (GPR119) has received much interest from the pharmaceutical industry. This interest peaked in June 2010, when Sanofi-Aventis agreed to pay Metabolex (Cymabay Therapeutics) US$375 million for MBX-2982, which was a representative orally active GPR119 agonist. However, Sanofi-Aventis opted to terminate the deal in May 2011 and another leading GPR119 agonist, GSK1292263, had a loss of efficacy during its clinical trial. In this review, I discuss the pros and cons of GPR119 through a strengths, weaknesses, opportunities, and threats (SWOT) analysis and propose development strategies for the eventual success of a GPR119 agonist development program.

  14. Administration of pioglitazone alone or with alogliptin delays diabetes onset in UCD-T2DM rats.

    Science.gov (United States)

    Cummings, Bethany P; Bettaieb, Ahmed; Graham, James L; Stanhope, Kimber; Haj, Fawaz G; Havel, Peter J

    2014-04-01

    There is a need to identify strategies for type 2 diabetes prevention. Therefore, we investigated the efficacy of pioglitazone and alogliptin alone and in combination to prevent type 2 diabetes onset in UCD-T2DM rats, a model of polygenic obese type 2 diabetes. At 2 months of age, rats were divided into four groups: control, alogliptin (20 mg/kg per day), pioglitazone (2.5 mg/kg per day), and alogliptin+pioglitazone. Non-fasting blood glucose was measured weekly to determine diabetes onset. Pioglitazone alone and in combination with alogliptin lead to a 5-month delay in diabetes onset despite promoting increased food intake and body weight (BW). Alogliptin alone did not delay diabetes onset or affect food intake or BW relative to controls. Fasting plasma glucose, insulin, and lipid concentrations were lower and adiponectin concentrations were threefold higher in groups treated with pioglitazone. All treatment groups demonstrated improvements in glucose tolerance and insulin secretion during an oral glucose tolerance test with an additive improvement observed with alogliptin+pioglitazone. Islet histology revealed an improvement of islet morphology in all treatment groups compared with control. Pioglitazone treatment also resulted in increased expression of markers of mitochondrial biogenesis in brown adipose tissue and white adipose tissue, with mild elevations observed in animals treated with alogliptin alone. Pioglitazone markedly delays the onset of type 2 diabetes in UCD-T2DM rats through improvements of glucose tolerance, insulin sensitivity, islet function, and markers of adipose mitochondrial biogenesis; however, addition of alogliptin at a dose of 20 mg/kg per day to pioglitazone treatment does not enhance the prevention/delay of diabetes onset.

  15. A STUDY ON CORRELATION OF DIABETIC RETINOPATHY IN RELATION TO DIABETIC NEPHROPATHY IN TYPE II DM PATIENTS

    Directory of Open Access Journals (Sweden)

    Yalaka Jayapal

    2015-08-01

    Full Text Available INTRODUCTION : Diabetes mellitus is one of the most common metabolic disorders of multiple etiology. The multisystem effects of diabetes such as retinopathy, nephropathy, neuropathy and cardiovascular diseases have an important impinging on the working age individuals in our country. Diabetic Retinopathy (DR is one of the leading c auses of blindness in the world that increases the chance of losing vision to about 25 times higher compare to normal individuals. Diabetic Nephropathy (DN is the major life - threatening complication which develops in approximately 20% to 40% of type 1 and less than 20% of type 2 diabetic patients. DN is the leading known cause of End stage renal disease (ESRD . AIM AND OBJECTIVE : A systematic cross - sectional study was conducted in Sarojini Devi eye hospital to assess the correlation between Diabetic Retino pathy and Diabetic Nephropathy in Type II Diabetes Mellitus patients. METHODS AND MATERIAL S : A study was conducted on 108 Type II DM patients presenting to Sarojini Devi Eye hospital, Hyderabad and were assessed for Retinopathy and Nephropathy and Correlat ion between Retinopathy and Nephropathy was studied from December 2011 to December 2014. RESULTS : Out of 54 Diabetic Retinopathy patients, 28 (51.8% patients had DN, 26 patients (48.2% had no evidence of DN. Out of 54 Diabetic Nephropathy patients, 36(66 .6% had DR; 18(33.4% had No evidence of DR. CONCLUSION : The results of our study suggest that Diabetic Nephropathy has a positive association with the presence of Diabetic Retinopathy in persons with Type II DM.

  16. Casting Technology Development for SFR Metallic Fuel

    Energy Technology Data Exchange (ETDEWEB)

    Lee, C.T.; Oh, S.J.; Ryu, H.J.; Kim, K.H.; Lee, Y.S.; Kim, S.K.; Woo, Y.M.; Ko, Y.M.; Lee, C.B. [KAERI, 150 Deokjin-dong, Yuseong, Daejeon, 305-353 (Korea, Republic of)

    2009-06-15

    Fabrication technology of metallic fuel for sodium fast reactor (SFR) is being developed in Korea as a national mid- and long-term nuclear R and D program from 2007. The metallic fuel for SFR should be remotely fabricated under a radiation shielded environment such as a glove box or hot cell, because it contains long-lived minor actinides such as Np, Am and Cm. In order to design a reliable remote fabrication system, various casting techniques have been studied by using U-Zr and U-Zr-RE alloys as surrogate fuel material. Rare earth elements such as Ce or Nd were used as a surrogate for minor actinide elements or solid solution fission products. Macro-scale soundness, microstructures and compositional homogeneity of metallic fuel samples fabricated by vacuum-assisted injection casting, vacuum-assisted gravity casting, centrifugal atomization and continuous casting were compared. Although sound slugs of U-Zr metallic fuel of 4{approx}6 mm in diameter could be fabricated by vacuum-assisted injection casting or vacuum-assisted gravity casting, it was necessary to consider that vaporization of Am and volume of radioactive wastes such as crucibles and molds should be minimized. Effects of casting parameters on the volatile loss, and effects of coatings on the chemical reaction between metallic fuel and molds are discussed. Some methods to reduce the volatile Am loss and waste molds and crucibles will be proposed. Short rods of U-Zr or U-Zr-Ce fuel will be fabricated by the vacuum-assisted gravity casting technique for an irradiation test in the HANARO research reactor from 2010. (authors)

  17. Aproveitamento de subproduto industrial de óleos vegetais para produção de riboflavina por Candida guilliermondii DM 644 Utilization industrial waste from vegetal oils riboflavine production by candida guilliermondii DM 644

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Andrade Pessoa

    2003-12-01

    Full Text Available A produção e consumo de alimentos industrializados têm aumentado a preocupação com suplementação e enriquecimento de alimentos com vitaminas e sais minerais, visando repor as possíveis perdas durante os processos de fabricação, principalmente das vitaminas hidrossolúveis, mais especificamente da vitamina B2 ou riboflavina. Assim sendo, a proposta deste trabalho foi utilizar como componente principal do meio, para produção da riboflavina, um subproduto do refino de óleos vegetais e o microrganismo Candida guillermondii DM 644. A produção da vitamina B2 foi realizada por fermentação em batelada utilizando Erlenmeyer. As condições empregadas foram agitação orbital, ausência de luz, 30°C, e 24h de incubação. A otimização da produção de riboflavina foi realizada através de Delineamento Fatorial Fracionário, para avaliar os efeitos da concentração de matéria graxa, fonte de nitrogênio, pH, velocidade de agitação, fonte de fósforo e extrato de levedura e as possíveis interações. A concentração máxima de riboflavina foi 19,12mg/mL. Os fatores mais importantes para produção de riboflavina foram a concentração de matéria graxa e a fonte de nitrogênio, enquanto que a fonte de fósforo e o extrato de leveduras não estimularam sua biossíntese. A máxima produção foi obtida com matéria graxa a 10g/L, uréia a 2,5g/L e pH 5,0. A velocidade de agitação (200 e 400rpm não interferiu no processo biotecnológico.The ever growing production and consumption of industrialized foods have increased the concern about supplementation and enrichment of food with vitamins and minerals, attempting the replenishment of the possible losses during their production processes, mainly the hydrosoluble vitamins, more specifically B2 vitamin or riboflavin. A subproduct of vegetal oil refining and the microorganism Candida guilliermondii DM 644 were used as the main substrate. The vitamin B2 was produced by the batch

  18. EW baryogenesis via DM

    CERN Document Server

    Lewicki, Marek

    2016-01-01

    We discuss the impact of a swifter cosmological expansion induced by modified cosmological history of the universe on scenarios realising electroweak baryogenesis. We detail the possible experimental bounds one can place on such cosmological modification and show how the detection capabilities of particle models are modified within these bounds. On the particle physics side we focus on the Standard Model supplemented by a dimension six operator which directly modifies the Higgs potential. We show that due to the cosmological modification, electroweak baryogenesis in this model can be realized, with the modification of the triple Higgs coupling below HL-LHC sensitivity.

  19. Development and responses of brain metastases during treatment with trastuzumab emtansine (T-DM1) for HER2 positive advanced breast cancer: A single institution experience.

    Science.gov (United States)

    Okines, Alicia; Irfan, Tazia; Khabra, Komel; Smith, Ian; O'Brien, Mary; Parton, Marina; Noble, Jill; Stanway, Susie; Somaiah, Navita; Ring, Alistair; Johnston, Stephen; Turner, Nicholas

    2017-08-22

    Ado-trastuzumab emtansine (T-DM1) is an antibody-drug conjugate that does not cross an intact blood-brain barrier. In the EMILIA trial of T-DM1 vs capecitabine/lapatinib for HER2 positive advanced breast cancer, all patients had baseline brain imaging, and 9/450 (2%) of patients with negative baseline imaging developed new brain disease during T-DM1. We assessed the frequency of brain progression in clinical practice, without routine baseline imaging. We undertook a retrospective study of all patients treated with T-DM1 at the Royal Marsden Hospital from 2011 to 2016. Data collected included baseline characteristics, previous treatment for advanced breast cancer, sites of metastatic disease, duration of T-DM1, sites of progression, and treatment of CNS progression. Fifty-five patients were identified who had received a median of two prior lines of treatment (range 0-5). All were HER2 positive; 45 patients had IHC 3+ tumors and 10 were ISH positive. Patients received a median of 12 cycles of T-DM1 (range 1-34), and six remain on treatment at the time of analysis. Before commencing T-DM1, 16/55 (29%) had known brain metastases (treated with whole brain [9] stereotactic radiotherapy [6] or both [1]). Brain was the first site of progression in 56% (9/16) patients, with a median time to brain progression of 9.9 months (95% CI 3.9-12.2). In patients without known baseline brain metastases, 17.9% (7/39) developed new symptomatic brain disease during T-DM1, after a median of 7.5 months (95%CI 3.8-9.6). Brain progression was isolated, with control of extra-cranial disease in 4/7 patients. Only one patient was suitable for stereotactic radiotherapy. Median time to extra-cranial progression in all patients was 11.5 months (95% CI 9.1-17.7), and median OS in all patients was 17.8 months (95% CI 14.2-22). In patients not screened for brain metastases at baseline, the brain was the first site of progression in a significant proportion. Baseline brain imaging may have a role

  20. Aberrant Brain Regional Homogeneity and Functional Connectivity in Middle-Aged T2DM Patients: A Resting-State Functional MRI Study

    Science.gov (United States)

    Liu, Daihong; Duan, Shanshan; Zhang, Jiuquan; Zhou, Chaoyang; Liang, Minglong; Yin, Xuntao; Wei, Ping; Wang, Jian

    2016-01-01

    Type 2 diabetes mellitus (T2DM) has been associated with cognitive impairment. However, its neurological mechanism remains elusive. Combining regional homogeneity (ReHo) and functional connectivity (FC) analyses, the present study aimed to investigate brain functional alterations in middle-aged T2DM patients, which could provide complementary information for the neural substrates underlying T2DM-associated brain dysfunction. Twenty-five T2DM patients and 25 healthy controls were involved in neuropsychological testing and structural and resting-state functional magnetic resonance imaging (rs-fMRI) data acquisition. ReHo analysis was conducted to determine the peak coordinates of brain regions with abnormal local brain activity synchronization. Then, the identified brain regions were considered as seeds, and FC between these brain regions and global voxels was computed. Finally, the potential correlations between the imaging indices and neuropsychological data were also explored. Compared with healthy controls, T2DM patients exhibited higher ReHo values in the anterior cingulate gyrus (ACG) and lower ReHo in the right fusiform gyrus (FFG), right precentral gyrus (PreCG) and right medial orbit of the superior frontal gyrus (SFG). Considering these areas as seed regions, T2DM patients displayed aberrant FC, mainly in the frontal and parietal lobes. The pattern of FC alterations in T2DM patients was characterized by decreased connectivity and positive to negative or negative to positive converted connectivity. Digital Span Test (DST) forward scores revealed significant correlations with the ReHo values of the right PreCG (ρ = 0.527, p = 0.014) and FC between the right FFG and middle temporal gyrus (MTG; ρ = −0.437, p = 0.048). Our findings suggest that T2DM patients suffer from cognitive dysfunction related to spatially local and remote brain activity synchronization impairment. The patterns of ReHo and FC alterations shed light on the mechanisms underlying T2DM

  1. Lab-On-a-Chip Application Development (LOCAD): Bridging Technology Readiness for Exploration

    Science.gov (United States)

    Spearing, Scott F.; Jenkins, Andy

    2004-01-01

    At Marshall Space Flight Center we have established a capability to investigate the use of microfluidics for space flight. The Lab-On-a-Chip Application Development (LOCAD) team has created a program for advancing Technology Readiness Levels (TRL) of 1 and 2 to TRL 6 and 7, quickly and economically for Lab-On-a-Chip (LOC) applications. Scientists and engineers can utilize LOCAD'S process to efficiently learn about microfluidics and determine if microfluidics is applicable to their needs. Once the applicability has been determined, LOCAD can then perform tests to develop the new fluidic protocols which are different from macro-scale chemical reaction protocols. With this information new micro-fluidic devices can be created and tested. Currently, LOCAD is focused on using microfluidics for both Environmental Monitoring & Control, and Medical Systems. Eventually, handheld portable units utilizing LOC technology will perform rapid tests to determine water quality, and microbial contamination levels. Since LOC technology is drastically reduced in physical size, it thereby reduces power, weight, volume, and sample requirements, a big advantage considering the resource constraints associated with spaceflight. Another one of LOCAD's current activities is the development of a microfluidic system to aid in the search for life on Mars.

  2. Can Integrated Micro-Optical Concentrator Technology Revolutionize Flat-Plate Photovoltaic Solar Energy Harvesting?

    Science.gov (United States)

    Haney, Michael W.

    2015-12-01

    The economies-of-scale and enhanced performance of integrated micro-technologies have repeatedly delivered disruptive market impact. Examples range from microelectronics to displays to lighting. However, integrated micro-scale technologies have yet to be applied in a transformational way to solar photovoltaic panels. The recently announced Micro-scale Optimized Solar-cell Arrays with Integrated Concentration (MOSAIC) program aims to create a new paradigm in solar photovoltaic panel technology based on the incorporation of micro-concentrating photo-voltaic (μ-CPV) cells. As depicted in Figure 1, MOSAIC will integrate arrays of micro-optical concentrating elements and micro-scale PV elements to achieve the same aggregated collection area and high conversion efficiency of a conventional (i.e., macro-scale) CPV approach, but with the low profile and mass, and hopefully cost, of a conventional non-concentrated PV panel. The reduced size and weight, and enhanced wiring complexity, of the MOSAIC approach provide the opportunity to access the high-performance/low-cost region between the conventional CPV and flat-plate (1-sun) PV domains shown in Figure 2. Accessing this portion of the graph in Figure 2 will expand the geographic and market reach of flat-plate PV. This talk reviews the motivation and goals for the MOSAIC program. The diversity of the technical approaches to micro-concentration, embedded solar tracking, and hybrid direct/diffuse solar resource collection found in the MOSAIC portfolio of projects will also be highlighted.

  3. sfDM: Open-Source Software for Temporal Analysis and Visualization of Brain Tumor Diffusion MR Using Serial Functional Diffusion Mapping.

    Science.gov (United States)

    Ceschin, Rafael; Panigrahy, Ashok; Gopalakrishnan, Vanathi

    2015-01-01

    A major challenge in the diagnosis and treatment of brain tumors is tissue heterogeneity leading to mixed treatment response. Additionally, they are often difficult or at very high risk for biopsy, further hindering the clinical management process. To overcome this, novel advanced imaging methods are increasingly being adapted clinically to identify useful noninvasive biomarkers capable of disease stage characterization and treatment response prediction. One promising technique is called functional diffusion mapping (fDM), which uses diffusion-weighted imaging (DWI) to generate parametric maps between two imaging time points in order to identify significant voxel-wise changes in water diffusion within the tumor tissue. Here we introduce serial functional diffusion mapping (sfDM), an extension of existing fDM methods, to analyze the entire tumor diffusion profile along the temporal course of the disease. sfDM provides the tools necessary to analyze a tumor data set in the context of spatiotemporal parametric mapping: the image registration pipeline, biomarker extraction, and visualization tools. We present the general workflow of the pipeline, along with a typical use case for the software. sfDM is written in Python and is freely available as an open-source package under the Berkley Software Distribution (BSD) license to promote transparency and reproducibility.

  4. Impact of faecal DM excretion on faecal calcium losses in dogs eating complete moist and dry pet foods - food digestibility is a major determinant of calcium requirements.

    Science.gov (United States)

    Kienzle, Ellen; Brenten, Thomas; Dobenecker, Britta

    2017-01-01

    The recommendations for the Ca supply for maintenance of dogs have been reduced by about 75 % in the last decades. An important factor for Ca requirements is faecal Ca losses. In previous studies with experimental diets faecal Ca losses depended on Ca intake and on faecal DM excretion. A predictive equation for faecal Ca losses in mg/kg body weight (BW) developed in a fibre model is: faecal losses = -33·8 + (13·6 faecal DM excretion (g/kg BW)) + (0·78 Ca intake (mg/kg BW)). The present study aimed at testing this equation in pet food with material from trials carried out for other purposes. Digestion trials with twenty-five dry and fifteen moist foods (326 observations in total) were evaluated retrospectively. Faecal DM excretion and faecal Ca losses were significantly correlated (r(2) 0·86; P food. Faecal DM excretion has a considerable impact on faecal Ca losses in a practical feeding situation. In conclusion, Ca requirements for maintenance may vary with food DM intake and digestibility.

  5. Early Vascular Damage in Young Women with DM-1 and Its Relation to Anti-Müllerian Hormone: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Annelien C. de Kat

    2016-01-01

    Full Text Available Vascular function is suggested to be associated with ovarian reserve, but the relationship with microvascular function has never been studied. In this cross-sectional pilot study, the relationship of microvascular damage markers with AMH was studied in premenopausal women. Twenty-two regularly cycling women with type 1 diabetes (DM-1 and a reference group of 20 healthy regularly cycling women were included, from whom blood was drawn in the early follicular phase of the menstrual cycle. The main outcome was the correlation between circulating progenitor cells (CPCs, markers for early vascular damage, and AMH, a marker for ovarian reserve. Secondary endpoints for early vascular impairment were circulating angiogenic cells and additional biomarkers. Median AMH levels were 2.2 µg/L [1.2–3.5] in the DM-1 group and 2.1 µg/L [0.85–3.8] in the reference group. CPCs were significantly decreased in women with DM-1; 1204±537 CD34+/CD45dim cells were counted in the DM-1 group, compared to 2264±1124 in the reference group. CPCs and other markers of early vascular damage were not correlated with AMH levels in a multivariable analysis. These results underscore previous findings of early vascular damage in DM-1 and suggest that there may not be a relationship between vascular function and ovarian reserve. Trial Registration. This trial is registered with Clinicaltrials.gov NCT01665716.

  6. 使用DSP/BIOS开发DM 642图像融合系统驱动程序%Using DSP/BIOS in Driver Development of the Image Fusion System Based on DM 642

    Institute of Scientific and Technical Information of China (English)

    王强; 倪国强; 李江涛; 宋亚军

    2006-01-01

    描述了在自主研制的TMS320DM642图像融合系统平台上,使用实时多线程操作系统(DSP/BIOS)开发驱动程序和编写融合算法的方法和流程.综合运用了BIOS、CSL、BSL、DDK、RF5、XDIAS等技术编写设备驱动程序,使用标准的类/微型驱动程序模型开发新的视频驱动程序,实现了软件开发的模块化和层次化.在此基础上编写了图像融合程序,总结了CCS编程中使用的各项优化技术.与传统软件开发比较,基于DSP/BIOS的DSP驱动程序缩短了开发时间,增加了代码的兼容性、继承性和可移植性.

  7. Evaluation of new alternatives in wastewater treatment plants based on dynamic modelling and life cycle assessment (DM-LCA).

    Science.gov (United States)

    Bisinella de Faria, A B; Spérandio, M; Ahmadi, A; Tiruta-Barna, L

    2015-11-01

    With a view to quantifying the energy and environmental advantages of Urine Source-Separation (USS) combined with different treatment processes, five wastewater treatment plant (WWTP) scenarios were compared to a reference scenario using Dynamic Modelling (DM) and Life Cycle Assessment (LCA), and an integrated DM-LCA framework was thus developed. Dynamic simulations were carried out in BioWin(®) in order to obtain a realistic evaluation of the dynamic behaviour and performance of plants under perturbation. LCA calculations were performed within Umberto(®) using the Ecoinvent database. A Python™ interface was used to integrate and convert simulation data and to introduce them into Umberto(®) to achieve a complete LCA evaluation comprising foreground and background processes. Comparisons between steady-state and dynamic simulations revealed the importance of considering dynamic aspects such as nutrient and flow peaks. The results of the evaluation highlighted the potential of the USS scenario for nutrient recovery whereas the Enhanced Primary Clarification (EPC) scenario gave increased biogas production and also notably decreased aeration consumption, leading to a positive energy balance. Both USS and EPC scenarios also showed increased stability of plant operation, with smaller daily averages of total nitrogen and phosphorus. In this context, USS and EPC results demonstrated that the coupled USS + EPC scenario and its combinations with agricultural spreading of N-rich effluent and nitritation/anaerobic deammonification could present an energy-positive balance with respectively 27% and 33% lower energy requirements and an increase in biogas production of 23%, compared to the reference scenario. The coupled scenarios also presented lesser environmental impacts (reduction of 31% and 39% in total endpoint impacts) along with effluent quality well within the specified limits. The marked environmental performance (reduction of global warming) when nitrogen is used

  8. Historical review: the carbon monoxide diffusing capacity (DLCO) and its membrane (DM) and red cell (Theta.Vc) components.

    Science.gov (United States)

    Hughes, J M B; Bates, D V

    2003-11-14

    The single breath carbon monoxide diffusing capacity (DLCO sb), also called the transfer factor (TLCO), was introduced by Marie and August Krogh in two papers (Krogh and Krogh, Skand. Arch. Physiol. 23, 236-247, 1909; Krogh, J. Physiol., Lond. 49, 271-296, 1915). Physiologically, their measurements showed that sufficient oxygen (by extrapolation from CO) diffused passively from gas to blood without the need to postulate oxygen secretion, a popular theory at the time. Their DLCO sb technique was neglected until the advent of the infra-red CO meter in the 1950s. Ogilvie et al., J. Clin. Invest. 36, 1-17, 1957 published a standardized technique for a 'modified Krogh' single breath DLCO, which eventually became the method of choice in pulmonary function laboratories. The Roughton-Forster equation (J. Appl. Physiol. 1957, 11, 290-302) was an important step conceptually; it partitioned alveolar-capillary diffusion of oxygen (O2) and carbon monoxide (CO) into a membrane component (DM) and a red cell component (theta.Vc) where theta is the DLCO (or DL(O2)) per ml of blood (measured in vitro), and Vc is the pulmonary capillary volume. This equation was based on the kinetics of O2 and CO with haemoglobin (Hb) in solution and with whole blood Hartridge and Roughton, Nature, 1923, 111, 325-326; Proc. R. Soc. Lond. Ser. A, 1923, 104, 376-394; (Proc. R. Soc. Lond. Ser. B, 1923, 94, 336-367; Proc. R. Soc. Lond. Ser. A 1923, 104, 395-430; J. Physiol., Lond. 1927, 62, 232-242; Roughton, Proc. R. Soc. Lond. Ser. B 1932, 111, 1-36) and on the relationship between alveolar P(O2) and 1/DLCO. Subsequently, the relationship between DL(O2) (Lilienthal et al., Am. J. Physiol. 147, 199-216, 1946) and DL(CO) was defined. More recently, the measurement of the nitric oxide diffusing capacity (DLNO) has been introduced. For DL(O2) and DLNO the membrane component (as 1/DM) is an important part of the overall diffusion (transfer) resistance. For the DLCO, 1/theta.Vc probably plays the greater

  9. First Search for a Dark Matter Annual Modulation Signal with NaI(Tl) in the Southern Hemisphere by DM-Ice17

    Energy Technology Data Exchange (ETDEWEB)

    Barbosa de Souza, E.; et al.

    2016-02-18

    The first search for a dark matter annual modulation signal with NaI(Tl) target material in the Southern Hemisphere conducted with the DM-Ice17 experiment is presented. DM-Ice17 consists of 17 kg of NaI(Tl) scintillating crystal under 2200 m.w.e. overburden of Antarctic glacial ice. The analysis presented here utilizes a 60.8 kg yr exposure. While unable to exclude the signal reported by DAMA/LIBRA, the DM-Ice17 data are consistent with no modulation in the energy range of 4-20 keV, providing the strongest limits on WIMP candidates from a direct detection experiment located in the Southern Hemisphere. Additionally, the successful deployment and stable operation of 17 kg of NaI(Tl) crystal over 3.5 years establishes the South Pole ice as a viable location for future underground, low-background experiments.

  10. First Search for a Dark Matter Annual Modulation Signal with NaI(Tl) in the Southern Hemisphere by DM-Ice17

    CERN Document Server

    de Souza, E Barbosa; Cole, A; Ezeribe, A C; Grant, D; Halzen, F; Heeger, K M; Hsu, L; Hubbard, A J F; Jo, J H; Karle, A; Kauer, M; Kudryavtsev, V A; Lim, K E; Macdonald, C; Maruyama, R H; Mouton, F; Paling, S M; Pettus, W; Pierpoint, Z P; Reilly, B N; Robinson, M; Rogers, F R; Sandstrom, P; Scarff, A; Spooner, N J C; Telfer, S; Yang, L

    2016-01-01

    The first search for a dark matter annual modulation signal with NaI(Tl) target material in the Southern Hemisphere conducted with the DM-Ice17 experiment is presented. DM-Ice17 consists of 17 kg of NaI(Tl) scintillating crystal under 2200 m.w.e. overburden of Antarctic glacial ice. The analysis presented here utilizes a 60.8 kg yr exposure. While unable to exclude the signal reported by DAMA/LIBRA, the DM-Ice17 data are consistent with no modulation in the energy range of 4-20 keV, providing the strongest limits on WIMP candidates from a direct detection experiment located in the Southern Hemisphere. Additionally, the successful deployment and stable operation of 17 kg of NaI(Tl) crystal over 3.5 years establishes the South Pole ice as a viable location for future underground, low-background experiments.

  11. [Levels of some auto-antibodies and C-peptide in insulin-treated patients with diabetes mellitus (DM) depending on gender of the patients and disease duration].

    Science.gov (United States)

    Likhonosov, P N; Khalangot, N D

    2007-01-01

    Determination of antibodies to thyroid peroxidase was recently proposed to single out subgroups among patients with DM1 for following clinical and genetic studies. 73 men and 64 women who are being treated with insulin and included in territorial register of patients with DM of Mariupol city, Donetsk region, Ukraine. Average age of patients at the stage of investigation was 35.17+/-1.16, the duration of the disease was 11.85+/-0.74, BMI-23.13+/-0.32 kg/m2, HBA1c9.23+/-0.25, C-peptide 0.48+/-0.09 nmol/l, glutaminic acid decarboxylase antibodies-GADAs-0.63+/-0.04 units/ml and did not depend on gender. Patients aged less then 30 years had their level of GADAbs and TPOAbs changed linearly correlated with the duration of diabetes mellitus. Obtained data can be used to assess subgroups among patients with DM1.

  12. Curcumin inhibits growth potential by G1 cell cycle arrest and induces apoptosis in p53-mutated COLO 320DM human colon adenocarcinoma cells.

    Science.gov (United States)

    Dasiram, Jade Dhananjay; Ganesan, Ramamoorthi; Kannan, Janani; Kotteeswaran, Venkatesan; Sivalingam, Nageswaran

    2017-02-01

    Curcumin, a natural polyphenolic compound and it is isolated from the rhizome of Curcuma longa, have been reported to possess anticancer effect against stage I and II colon cancer. However, the effect of curcumin on colon cancer at Dukes' type C metastatic stage III remains still unclear. In the present study, we have investigated the anticancer effects of curcumin on p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. The cellular viability and proliferation were assessed by trypan blue exclusion assay and MTT assay, respectively. The cytotoxicity effect was examined by lactate dehydrogenase (LDH) cytotoxicity assay. Apoptosis was analyzed by DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis. Cell cycle distribution was performed by flow cytometry analysis. Here we have observed that curcumin treatment significantly inhibited the cellular viability and proliferation potential of p53 mutated COLO 320DM cells in a dose- and time-dependent manner. In addition, curcumin treatment showed no cytotoxic effects to the COLO 320DM cells. DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis revealed that curcumin treatment induced apoptosis in COLO 320DM cells. Furthermore, curcumin caused cell cycle arrest at the G1 phase, decreased the cell population in the S phase and induced apoptosis in COLO 320DM colon adenocarcinoma cells. Together, these data suggest that curcumin exerts anticancer effects and induces apoptosis in p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage.

  13. The novel adjuvant dmLT promotes dose sparing, mucosal immunity and longevity of antibody responses to the inactivated polio vaccine in a murine model.

    Science.gov (United States)

    Norton, Elizabeth B; Bauer, David L; Weldon, William C; Oberste, M Steven; Lawson, Louise B; Clements, John D

    2015-04-15

    One option for achieving global polio eradication is to replace the oral poliovirus vaccine (OPV), which has the risk of reversion to wild-type virulence, with the inactivated poliovirus vaccine (IPV) vaccine. Adjuvants and alternate routes of immunization are promising options that may reduce antigen dose in IPV vaccinations, potentially allowing dose sparing and cost savings. Use of adjuvants and alternate routes of immunization could also help promote mucosal immunity, potentially mimicking the protection against intestinal virus shedding seen with OPV. In the current study, we examined the impact of combining the novel adjuvant dmLT with trivalent IPV for dose sparing, induction of mucosal immunity and increasing longevity of anti-poliovirus (PV) responses in a mouse model following either intradermal (ID) or intramuscular (IM) delivery. We found that non-adjuvanted ID delivery was not superior to IM delivery for fractional dose sparing, but was associated with development of mucosal immunity. Vaccination with IPV+dmLT promoted serum anti-PV neutralizing antibodies with fractional IPV doses by either IM or ID delivery, achieving at least five-fold dose sparing above non-adjuvanted fractional doses. These responses were most noticeable with the PV1 component of the trivalent vaccine. dmLT also promoted germinal center formation and longevity of serum anti-PV neutralizing titers. Lastly, dmLT enhanced mucosal immunity, as defined by fecal and intestinal anti-PV IgA secretion, when included in IPV immunization by ID or IM delivery. These studies demonstrate that dmLT is an effective adjuvant for either IM or ID delivery of IPV. Inclusion of dmLT in IPV immunizations allows antigen dose sparing and enhances mucosal immunity and longevity of anti-PV responses.

  14. Attitudes and barriers to exercise in adults with type 1 diabetes (T1DM and how best to address them: a qualitative study.

    Directory of Open Access Journals (Sweden)

    Nadia Lascar

    Full Text Available Regular physical activity has recognised health benefits for people with T1DM. However a significant proportion of them do not undertake the recommended levels of activity. Whilst questionnaire-based studies have examined barriers to exercise in people with T1DM, a formal qualitative analysis of these barriers has not been undertaken. Our aims were to explore attitudes, barriers and facilitators to exercise in patients with T1DM.A purposeful sample of long standing T1DM patients were invited to participate in this qualitative study. Twenty-six adults were interviewed using a semi-structured interview schedule to determine their level of exercise and barriers to initiation and maintenance of an exercise programme.Six main barriers to exercise were identified: lack of time and work related factors; access to facilities; lack of motivation; embarrassment and body image; weather; and diabetes specific barriers (low levels of knowledge about managing diabetes and its complications around exercise. Four motivators to exercise were identified: physical benefits from exercise; improvements in body image; enjoyment and the social interaction of exercising at gym or in groups. Three facilitators to exercise were identified: free or reduced admission to gyms and pools, help with time management, and advice and encouragement around managing diabetes for exercise.Many of the barriers to exercise in people with T1DM are shared with the non-diabetic population. The primary difference is the requirement for education about the effect of exercise on diabetes control and its complications. There was a preference for support to be given on a one to one basis rather than in a group environment. This suggests that with the addition of the above educational requirements, one to one techniques that have been successful in increasing activity in patients with other chronic disease and the general public should be successful in increasing activity in patients with T1DM.

  15. TREX-DM: a low-background Micromegas-based TPC for low-mass WIMP detection

    CERN Document Server

    Iguaz, F J; Aznar, F; Castel, J F; Cebrián, S; Dafni, T; García, J A; Irastorza, I G; Lagraba, A; Luzón, G; Peiró, A

    2015-01-01

    If Dark Matter is made of Weakly Interacting Massive Particles (WIMPs) with masses below 10-20 GeV, the corresponding nuclear recoils in mainstream WIMP experiments are of energies too close, or below, the experimental threshold. New detection techniques, focused on the use of light target nuclei together with low energy thresholds, are needed to get competitive sensitivity to the low-mass range of the WIMP parameter space. Gas Time Projection Chambers (TPCs) can be operated with a variety of target elements, and on account of the amplification in gas, very low thresholds are achievable. Recent advances in electronics and in novel radiopure TPC readouts, especially micro-mesh gas structure (Micromegas) are improving the scalability and low-background prospects of Micromegas-based TPCs. If we add their well-known tracking capabilities, they are a good detection option for the search of low-mass WIMPs. Here we present TREX-DM, a prototype built to test this concept. The detector is designed to host an active ma...

  16. Understanding the Core-Halo Relation of Quantum Wave Dark Matter, $\\psi$DM, from 3D Simulations

    CERN Document Server

    Schive, Hsi-Yu; Woo, Tak-Pong; Wong, Shing-Kwong; Chiueh, Tzihong; Broadhurst, Tom; Hwang, W-Y Pauchy

    2014-01-01

    We examine the nonlinear structure of gravitationally collapsed objects that form in our simulations of wavelike cold dark matter ($\\psi$DM), described by the Schr\\"odinger-Poisson (SP) equation. A distinct gravitationally self-bound solitonic core is found at the center of every halo, with a profile quite different from cores modeled in the warm or self-interacting dark matter scenarios. Furthermore, we show that each solitonic core is surrounded by an extended halo composed of large fluctuating dark matter granules which modulate the halo density on a scale comparable to the diameter of the solitonic core. The scaling symmetry of the SP equation and the uncertainty principle tightly relate the core mass to the halo specific energy, which, in the context of cosmological structure formation, leads to a simple scaling between core mass ($M_c$) and halo mass ($M_h$), $M_c \\propto a^{-1/2} M_h^{1/3}$, where $a$ is the cosmic scale factor. We verify this scaling relation by (i) examining the internal structure of...

  17. TREX-DM: a low-background Micromegas-based TPC for low-mass WIMP detection

    Energy Technology Data Exchange (ETDEWEB)

    Iguaz, F.J.; Garza, J.G.; Castel, J.F.; Cebrian, S.; Dafni, T.; Garcia, J.A.; Irastorza, I.G.; Lagraba, A.; Luzon, G.; Peiro, A. [Universidad de Zaragoza, Grupo de Fisica Nuclear y Astroparticulas, Zaragoza (Spain); Aznar, F. [Universidad de Zaragoza, Grupo de Fisica Nuclear y Astroparticulas, Zaragoza (Spain); Universidad de Zaragoza, Centro Universitario de la Defensa, Zaragoza (Spain)

    2016-10-15

    If Dark Matter is made of Weakly Interacting Massive Particles (WIMPs) with masses below ∝20 GeV, the corresponding nuclear recoils in mainstream WIMP experiments are of energies too close, or below, the experimental threshold. Gas Time Projection Chambers (TPCs) can be operated with a variety of target elements, offer good tracking capabilities and, on account of the amplification in gas, very low thresholds are achievable. Recent advances in electronics and in novel radiopure TPC readouts, especially micro-mesh gas structure (Micromegas), are improving the scalability and low-background prospects of gaseous TPCs. Here we present TREX-DM, a prototype to test the concept of a Micromegas-based TPC to search for low-mass WIMPs. The detector is designed to host an active mass of ∝0.300 kg of Ar at 10 bar, or alternatively ∝0.160 kg of Ne at 10 bar, with an energy threshold below 0.4 keVee, and is fully built with radiopure materials. We will describe the detector in detail, the results from the commissioning phase on surface, as well as a preliminary background model. The anticipated sensitivity of this technique may go beyond current experimental limits for WIMPs of masses of 2-8 GeV. (orig.)

  18. Kepler Flares. IV. A Comprehensive Analysis of the Activity of the dM4e Star GJ 1243

    Science.gov (United States)

    Silverberg, Steven M.; Kowalski, Adam F.; Davenport, James R. A.; Wisniewski, John P.; Hawley, Suzanne L.; Hilton, Eric J.

    2016-10-01

    We present a comprehensive study of the active dM4e star GJ 1243. We use previous observations and ground-based echelle spectroscopy to determine that GJ 1243 is a member of the Argus association of field stars, suggesting it is ∼ 30{--}50 {{Myr}} old. We analyze 11 months of 1 minute cadence data from Kepler, presenting Kepler flare frequency distributions, as well as determining correlations between flare energy, amplitude, duration, and decay time. We find that the exponent α of the power-law flare energy distribution varies in time, primarily due to completeness of sample and the low frequency of high-energy flares. We also find a deviation from a single power law at high energy. We use ground-based spectroscopic observations that were simultaneous with the Kepler data to provide simultaneous photometric and spectroscopic analysis of three low-energy flares, the lowest-energy dMe flares with detailed spectral analysis to date on any star. The spectroscopic data from these flares extend constraints for radiative hydrodynamic flare models to a lower energy regime than has previously been studied. We use this simultaneous spectroscopy and Kepler photometry to develop approximate conversions from the Kepler bandpass to the traditional U and B bands. This conversion will be a critical factor in comparing any Kepler flare analyses to the canon of previous ground-based flare studies.

  19. TREX-DM: a low-background Micromegas-based TPC for low-mass WIMP detection

    Science.gov (United States)

    Iguaz, F. J.; Garza, J. G.; Aznar, F.; Castel, J. F.; Cebrián, S.; Dafni, T.; García, J. A.; Irastorza, I. G.; Lagraba, A.; Luzón, G.; Peiró, A.

    2016-10-01

    If Dark Matter is made of Weakly Interacting Massive Particles (WIMPs) with masses below {˜ }20 GeV, the corresponding nuclear recoils in mainstream WIMP experiments are of energies too close, or below, the experimental threshold. Gas Time Projection Chambers (TPCs) can be operated with a variety of target elements, offer good tracking capabilities and, on account of the amplification in gas, very low thresholds are achievable. Recent advances in electronics and in novel radiopure TPC readouts, especially micro-mesh gas structure (Micromegas), are improving the scalability and low-background prospects of gaseous TPCs. Here we present TREX-DM, a prototype to test the concept of a Micromegas-based TPC to search for low-mass WIMPs. The detector is designed to host an active mass of {˜ }0.300 kg of Ar at 10 bar, or alternatively {˜ }0.160 kg of Ne at 10 bar, with an energy threshold below 0.4 keVee, and is fully built with radiopure materials. We will describe the detector in detail, the results from the commissioning phase on surface, as well as a preliminary background model. The anticipated sensitivity of this technique may go beyond current experimental limits for WIMPs of masses of 2-8 GeV.

  20. The transcriptome of the reference potato genome Solanum tuberosum Group Phureja clone DM1-3 516R44.

    Science.gov (United States)

    Massa, Alicia N; Childs, Kevin L; Lin, Haining; Bryan, Glenn J; Giuliano, Giovanni; Buell, C Robin

    2011-01-01

    Advances in molecular breeding in potato have been limited by its complex biological system, which includes vegetative propagation, autotetraploidy, and extreme heterozygosity. The availability of the potato genome and accompanying gene complement with corresponding gene structure, location, and functional annotation are powerful resources for understanding this complex plant and advancing molecular breeding efforts. Here, we report a reference for the potato transcriptome using 32 tissues and growth conditions from the doubled monoploid Solanum tuberosum Group Phureja clone DM1-3 516R44 for which a genome sequence is available. Analysis of greater than 550 million RNA-Seq reads permitted the detection and quantification of expression levels of over 22,000 genes. Hierarchical clustering and principal component analyses captured the biological variability that accounts for gene expression differences among tissues suggesting tissue-specific gene expression, and genes with tissue or condition restricted expression. Using gene co-expression network analysis, we identified 18 gene modules that represent tissue-specific transcriptional networks of major potato organs and developmental stages. This information provides a powerful resource for potato research as well as studies on other members of the Solanaceae family.

  1. Realization of a single image haze removal system based on DaVinci DM6467T processor

    Science.gov (United States)

    Liu, Zhuang

    2014-10-01

    Video monitoring system (VMS) has been extensively applied in domains of target recognition, traffic management, remote sensing, auto navigation and national defence. However the VMS has a strong dependence on the weather, for instance, in foggy weather, the quality of images received by the VMS are distinct degraded and the effective range of VMS is also decreased. All in all, the VMS performs terribly in bad weather. Thus the research of fog degraded images enhancement has very high theoretical and practical application value. A design scheme of a fog degraded images enhancement system based on the TI DaVinci processor is presented in this paper. The main function of the referred system is to extract and digital cameras capture images and execute image enhancement processing to obtain a clear image. The processor used in this system is the dual core TI DaVinci DM6467T - ARM@500MHz+DSP@1GH. A MontaVista Linux operating system is running on the ARM subsystem which handles I/O and application processing. The DSP handles signal processing and the results are available to the ARM subsystem in shared memory.The system benefits from the DaVinci processor so that, with lower power cost and smaller volume, it provides the equivalent image processing capability of a X86 computer. The outcome shows that the system in this paper can process images at 25 frames per second on D1 resolution.

  2. The Transcriptome of the Reference Potato Genome Solanum tuberosum Group Phureja Clone DM1-3 516R44

    Science.gov (United States)

    Massa, Alicia N.; Childs, Kevin L.; Lin, Haining; Bryan, Glenn J.; Giuliano, Giovanni; Buell, C. Robin

    2011-01-01

    Advances in molecular breeding in potato have been limited by its complex biological system, which includes vegetative propagation, autotetraploidy, and extreme heterozygosity. The availability of the potato genome and accompanying gene complement with corresponding gene structure, location, and functional annotation are powerful resources for understanding this complex plant and advancing molecular breeding efforts. Here, we report a reference for the potato transcriptome using 32 tissues and growth conditions from the doubled monoploid Solanum tuberosum Group Phureja clone DM1-3 516R44 for which a genome sequence is available. Analysis of greater than 550 million RNA-Seq reads permitted the detection and quantification of expression levels of over 22,000 genes. Hierarchical clustering and principal component analyses captured the biological variability that accounts for gene expression differences among tissues suggesting tissue-specific gene expression, and genes with tissue or condition restricted expression. Using gene co-expression network analysis, we identified 18 gene modules that represent tissue-specific transcriptional networks of major potato organs and developmental stages. This information provides a powerful resource for potato research as well as studies on other members of the Solanaceae family. PMID:22046362

  3. A Multi-Beam Radio Transient Detector With Real-Time De-Dispersion Over a Wide DM Range

    CERN Document Server

    Clarke, Nathan; Navarro, Robert; Trinh, Joseph

    2014-01-01

    Isolated, short dispersed pulses of radio emission of unknown origin have been reported and there is strong interest in wide-field, sensitive searches for such events. To achieve high sensitivity, large collecting area is needed and dispersion due to the interstellar medium should be removed. To survey a large part of the sky in reasonable time, a telescope that forms multiple simultaneous beams is desirable. We have developed a novel FPGA-based transient search engine that is suitable for these circumstances. It accepts short-integration-time spectral power measurements from each beam of the telescope, performs incoherent de-dispersion simultaneously for each of a wide range of dispersion measure (DM) values, and automatically searches the de-dispersed time series for pulse-like events. If the telescope provides buffering of the raw voltage samples of each beam, then our system can provide trigger signals to allow data in those buffers to be saved when a tentative detection occurs; this can be done with a la...

  4. Kepler Flares IV: A Comprehensive Analysis of the Activity of the dM4e Star GJ 1243

    CERN Document Server

    Silverberg, Steven M; Davenport, James R A; Wisniewski, John P; Hawley, Suzanne L; Hilton, Eric J

    2016-01-01

    We present a comprehensive study of the active dM4e star GJ 1243. We use previous observations and ground-based echelle spectroscopy to determine that GJ 1243 is a member of the Argus association of field stars, suggesting it is $\\sim 30-50$ Myr old. We analyze eleven months of 1-minute cadence data from Kepler, presenting Kepler flare frequency distributions, as well as determining correlations between flare energy, amplitude, duration, and decay time. We find that the exponent $\\alpha$ of the power-law flare energy distribution varies in time, primarily due to completeness of sample and the low frequency of high-energy flares. We also find a deviation from a single power law at high energy. We use ground-based spectroscopic observations simultaneous with the Kepler data to provide simultaneous photometric and spectroscopic analysis of three low-energy flares, the lowest-energy dMe flares with detailed spectral analysis to date on any star. The spectroscopic data from these flares extend constraints for radi...

  5. Patients Empowerment Based on Experimential Learning to Behavior of Acute Compilation Prevention and Blood Glucose Levels of Patients DM

    Directory of Open Access Journals (Sweden)

    Nunung Ernawati

    2015-10-01

    Full Text Available Introduction: Acute complications are common in patients with Diabetes Mellitus (DM. To improve the behavior prevention of complications and control blood sugar levels, patients need to be equipped with knowledge about the disease process to built a positive attitude and good behavior. The aim of this study was to analyze the effect of patient empowerment based on experiential learning behavior on the prevention of complications and blood sugar levels. Methods: This study used a quasi-experimental design with pre-post test approach using control groups. Samples were 46 diabetic patients who take control in poly RS Mardi Waluyo Blitar taken by consecutive sampling. Data were collected using a questionnaire and checklist recall. Data were analyzed using paired t test, wilcoxon signed rank test and mann whitney. Results: The patient empowerment-based experiential learning have a significant impact on the behavior of prevention of complications. Discussion: Methods of experiential learning can be applied to improve the self-care of patients, especially those who have experienced an acute complications, so the patient is easier to implement behavioral prevention of complications and control blood sugar levels. Keywords: patient empowerment, experiential learning, behavioral prevention, blood glucose

  6. Alteraciones neuropsicológicas asociadas a la diabetes mellitus 2 (dm2) y a sus factores de riesgo en población antioqueña

    OpenAIRE

    Gómez Muñoz, Natalia

    2011-01-01

    Ubicación en Biblioteca USB Medellín: CD-1914t.-- Grupo de Investigación en Salud Comportamental; Línea de Investigación: Neurociencia Cognitiva. La Diabetes Mellitus 2 (DM2) es un trastorno metabólico de múltiples etiologías. Los cambios en el estilo de vida, el envejecimiento progresivo de la población ha contribuido a un aumento significativo de la enfermedad, con una prevalencia mundial de población afectada de casi el 7%. La DM2 ha sido asociada a múltiples complicaciones microvascula...

  7. INTEGRATED DM 1200 MELTER TESTING OF HLW C-106/AY-102 COMPOSITION USING BUBBLERS VSL-03R3800-1 REV 0 9/15/03

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; KOT WK; PEGG IL

    2011-12-29

    This report documents melter and off-gas performance results obtained on the DM1200 HLW Pilot Melter during processing of simulated HLW C-106/AY-102 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW C-106/AY-102 feed; determine the effect of bubbling rate on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post test inspections of system components.

  8. MBNL142 and MBNL143 gene isoforms, overexpressed in DM1-patient muscle, encode for nuclear proteins interacting with Src family kinases.

    Science.gov (United States)

    Botta, A; Malena, A; Tibaldi, E; Rocchi, L; Loro, E; Pena, E; Cenci, L; Ambrosi, E; Bellocchi, M C; Pagano, M A; Novelli, G; Rossi, G; Monaco, H L; Gianazza, E; Pantic, B; Romeo, V; Marin, O; Brunati, A M; Vergani, L

    2013-08-15

    Myotonic dystrophy type-1 (DM1) is the most prevalent form of muscular dystrophy in adults. This disorder is an RNA-dominant disease, caused by expansion of a CTG repeat in the DMPK gene that leads to a misregulation in the alternative splicing of pre-mRNAs. The longer muscleblind-like-1 (MBNL1) transcripts containing exon 5 and the respective protein isoforms (MBNL142-43) were found to be overexpressed in DM1 muscle and localized exclusively in the nuclei. In vitro assays showed that MBNL142-43 bind the Src-homology 3 domain of Src family kinases (SFKs) via their proline-rich motifs, enhancing the SFK activity. Notably, this association was also confirmed in DM1 muscle and myotubes. The recovery, mediated by an siRNA target to Ex5-MBNL142-43, succeeded in reducing the nuclear localization of both Lyn and MBNL142-43 proteins and in decreasing the level of tyrosine phosphorylated proteins. Our results suggest an additional molecular mechanism in the DM1 pathogenesis, based on an altered phosphotyrosine signalling pathway.

  9. Lactuca saligna, a non-host for lettuce downy mildew (Bremia lactucae), harbors a new race-specific Dm gene and three QTL's for resistance

    NARCIS (Netherlands)

    Jeuken, M.J.W.; Lindhout, P.

    2002-01-01

    Lactuca sativa (lettuce) is susceptible to Bremia lactucae (downy mildew). In cultivated and wild Lactuca species, Dm genes have been identified that confer race-specific resistance. However, these genes were soon rendered ineffective by adaptation of the pathogen. Lactuca saligna (wild lettuce) is

  10. Health-related quality of life (HRQOL) and its associated factors in children with Type 1 Diabetes Mellitus (T1DM)

    NARCIS (Netherlands)

    Murillo, M. (Marta); Bel, J. (Joan); Pérez, J. (Jacobo); Corripio, R. (Raquel); Carreras, G. (Gemma); Herrero, X. (Xavier); Mengibar, J.-M. (Josep-Maria); D. Rodriguez-Arjona (Dolors); U. Ravens-Sieberer (Ulrike); H. Raat (Hein); L. Rajmil (Luis)

    2017-01-01

    textabstractBackground: The objective of the study was to describe the baseline health-related quality of life (HRQOL) in a cohort of children and adolescents with type 1 diabetes mellitus (T1DM), and analyze its associated clinical and sociodemographic factors, assessing HRQOL through internet. Met

  11. Anion size control of the packing in the metallic versus semiconducting chiral radical cation salts (DM-EDT-TTF)2XF6 (X = P, As, Sb).

    Science.gov (United States)

    Pop, Flavia; Auban-Senzier, Pascale; Canadell, Enric; Avarvari, Narcis

    2016-10-13

    Control of the structural type in metallic enantiopure and racemic radical cation salts is achieved through hydrogen bonding interactions between the chiral donor DM-EDT-TTF and the XF6 anions (X = P, As, Sb), determined by the anion size and the chiral information.

  12. Clinical Application of Urodilatin in Type 2 Diabetic Nephropathy%尿钠素在DM2-N中的变化及意义

    Institute of Scientific and Technical Information of China (English)

    朱易华; 曹兴建; 陈宇翔; 张克霞; 金艳

    2011-01-01

    目的:探讨尿钠素(URO)对2型糖尿病(DM2)早期肾小管损伤筛选的价值.方法:采用放免法检测正常对照组(41例)、DM2无肾病组(33例)、DM2早期糖尿病肾病(DN)组(37例)和DM2合并肾病组(26例)尿液中的URO,分析各组间URO水平的变化并与尿微量白蛋白与尿肌酐比值(mA/UCr)进行相关分析.URO诊断性能采用受试者工作特征(ROC)曲线进行分析.结果:在DM2无肾病组、早期肾病组和合并肾病组间,随着肾损伤程度的加深,患者尿URO的浓度出现显著性的减低(P<0.001);URO与mA/UCr间相关性分析显示,存在良好的负相关关系(r=-0.626,P<0.01).而且在DM2早期阶段,URO在ROC曲线的线下面积为0.759,取临界值≤51.5pg/ml时,其敏感性与特异性分别为67.14%和70.29%,与mA/UCr有相近的诊断效率.结论:尿液中URO排泄下降能特异性地反映DM2早期肾小管的损伤,并可作为重要的评估参数.%Objective To investigate the clinical application of urodilatin ( URO ) in tubular injury of DM2. Methods 41 healthy controls, 33 type 2 diabetics without nephropathy, 37 patients with early stage of diabetic nephropathy and 26 patients with clinical diabetic nephropathy were enrolled in the study and categorized into four groups. Urodilatin was measured by radioimmunoassay ( RIA ). The changes of urodilatin levels among four groups were analyzed, and correlation analyses were performed between urodilatin and urinary micro-albumin/urine creatinine ( mA/UCr ). The efficiency index of URO were evaluated by receiver operation characteristic ( ROC ). Results Compared with those in the controls, diabetics without nephropathy, early stage of diabetic nephropathy and clinical diabetic nephropathy, the urodilatin level decreased significantly in the course of diabetic nephropathy ( P<0.001 ). The value of URO was significantly correlated with mA/UCr ( r=-0.626, P<0.01 ). In early phase of DM2, The area under curve was 0.759. When the cut

  13. Dynamic Changes in the Intracellular Association of Selected Rab Small GTPases with MHC Class II and DM during Dendritic Cell Maturation.

    Science.gov (United States)

    Pérez-Montesinos, Gibrán; López-Ortega, Orestes; Piedra-Reyes, Jessica; Bonifaz, Laura C; Moreno, José

    2017-01-01

    Antigen processing for presentation by major histocompatibility complex class II (MHCII) molecules requires the latter to travel through the endocytic pathway together with its chaperons: the invariant chain (Ii) and DM. Nevertheless, the nature of the compartments where MHCII molecules travel to acquire peptides lacks definition regarding molecules involved in intracellular vesicular trafficking, such as Rab small GTPases. We aimed to define which Rab proteins are present during the intracellular transport of MHCII, DM, and Ii through the endocytic pathway on their route to the cell surface during dendritic cell (DC) maturation. We examined, by means of three-color confocal microscopy, the association of MHCII, DM, and Ii with Rab5, Rab7, Rab9, and Rab11 during the maturation of bone marrow-derived or spleen DC in response to LPS as an inflammatory stimulus. Prior to the stage of immature DC, MHCII migrated from diffuse small cytoplasmic vesicles, predominantly Rab5+Rab7- and Rab5+Rab7+ into a pericentriolar Rab5+Rab7+Rab9+ cluster, with Rab11+ areas. As DC reached the mature phenotype, MHCII left the pericentriolar endocytic compartments toward the cell surface in Rab11+ and Rab9+Rab11+ vesicles. The invariant chain and MHCII transport pathways were not identical. DM and MHCII appeared to arrive to pericentriolar endocytic compartments of immature DC through partially different routes. The association of MHCII molecules with distinct Rab GTPases during DC maturation suggests that after leaving the biosynthetic pathway, MHCII sequentially traffic from typical early endosomes to multivesicular late endosomes to finally arrive at the cell surface in Rab11+ recycling-type endosomes. In immature DCs, DM encounters transiently MHCII in the Rab5+Rab7+Rab9+ compartments, to remain there in mature DC.

  14. C-Peptide Is Independently Associated with an Increased Risk of Coronary Artery Disease in T2DM Subjects: A Cross-Sectional Study.

    Directory of Open Access Journals (Sweden)

    Lingshu Wang

    Full Text Available C-peptide has been reported to be a marker of subclinical atherosclerosis in type 2 diabetes mellitus (T2DM patients, whereas its role in coronary artery disease (CAD has not been clarified, especially in diabetics with differing body mass indices (BMIs.This cross-sectional study included 501 patients with T2DM. First, all subjects were divided into the following two groups: CAD and non-CAD. Then, binary logistic regression was used to determine the risk factors for CAD for all patients. To clarify the role of obesity, we re-divided all subjects into two additional groups (obese and non-obese based on BMI. Finally, binary logistic regression was used to determine the risk factors for CAD for each weight group.The patients with CAD showed a higher BMI and fasting C-peptide level in addition to an increased prevalence of traditional risk factors for CAD, such as hypertension, insulin resistance, higher cholesterol, cysteine-C (Cys-C and lower estimated glomerular filtration rate (eGFR. Logistic regression analysis showed that fasting C-peptide (OR=1.513, p=0.005, insulin treatment (OR=1.832, p=0.027 hypertension (OR=1.987, p=0.016 and hyperlipidemia (OR=4.159, p<0.001 significantly increased the risk of clinical CAD in the T2DM patients independent of age, gender, diabetes duration, smoking and alcohol statuses, fasting insulin and glucose, hypoglycemic episodes, UA and eGFR. Additionally, in both of the obese (OR=1.488, p=0.049 and non-obese (OR=1.686, p=0.037 DM groups, C-peptide was associated with an increased risk of CAD after multiple adjustments.C-peptide is associated with an increased CAD risk in T2DM patients, no matter whether they are obese or not.

  15. The effect of Liuwei Dihuang decoction on PI3K/Akt signaling pathway in liver of type 2 diabetes mellitus (T2DM) rats with insulin resistance.

    Science.gov (United States)

    Dai, Bing; Wu, Qinxuan; Zeng, Chengxi; Zhang, Jiani; Cao, Luting; Xiao, Zizeng; Yang, Menglin

    2016-11-04

    Liuwei Dihaung decoction (LWDHT) is a well-known classic traditional Chinese medicine formula, consists of six herbs including Rehmannia glutinosa Libosch.(family: Scrophulariaceae), Cornus officinalis Sieb.(family: Cornaceae), Dioscorea opposite Thunb.(family: Dioscoreaceae), Alisma orientale(G. Samuelsson) Juz (family: Alismataceae), Poria cocos (Schw.) Wolf (family: Polyporaceae) and Paeonia suffruticosa Andrews (family: Paeoniaceae). It has been used in the treatment of many types of diseases with signs of deficiency of Yin in the kidneys in China clinically. This study is aimed at investigating the effect of Liuwei dihuang decoction on PI3K/Akt signaling pathway in liver of T2DM rats with insulin resistance. T2DM model was induced in male Sprague-Dawley (SD) rats by high sugar and high fat diets combined with small dose of streptozocin (STZ) injection. The successful T2DM rats were randomly allocated three group--vehicle group, positive control group and Liuwei Dihuang decoction group. After 12-weeks treatment with distilled water, rosiglitazone and LWDHT by intragastric administration respectively, the rats were put to death in batches. The variance of fasting blood glucose (FBG) and fasting insulin (FINS) in serum were determined, the pathological changes of each rats' liver were observed by hematoxylin-eosin (HE) staining, the expression of insulin receptor substrate 2(IRS2), phosphatidylinositol 3-kinase (PI3K) and protein kinas B (Akt) involving the canonical PI3K/Akt signaling pathway were detected by Real-time fluorescent quantitative PCR (RT-PCR), and the expression level of IRS2, PI3K, Akt protein and phosphorylated IRS2, PI3K, Akt protein were evaluated by Western Blot. All the data were analyzed by SPSS 17.0. Four weeks of treatment with LWDHT could significantly decrease the level of FBG and FINS in serum, improve the cellular morphology of liver, kidney, pancreas tissue, and the expression of IRS2, PI3K, Akt mRNA and phosphorylated IRS2, PI3K, Akt

  16. Relations Between Atherogenic Index of Plasma, Ratio of Small Dense Low Density Lipoprotein/Lecithin Cholesterol Acyl Transferase and Ratio of Small Dense Low Density Lipoprotein/Cholesteryl Ester Transfer Protein of Controlled and Uncontrolled Type 2 DM

    Directory of Open Access Journals (Sweden)

    Ellis Susanti

    2009-08-01

    Full Text Available BACKGROUND: Patients with Diabetes Melitus are proven to be prone to atherosclerosis and coronary heart disease, especially type 2 Diabetes Melitus (T2DM patient who have higher risk and mortality for cardiovascular risk factor. The Dyslipidemia condition is very common in T2DM as one of the risk factors. Diabetic dyslipidemia is marked by the increased triglyceride (TG, low HDL cholesterol (HDL-C, and increased small dense LDL and apolipoprotein B. Therefore the aim of this study is to assess the differential and correlation between Atherogenic Index of Plasma (AIP, ratio of small dense low density lipoprotein (sdLDL/lecithin cholesterol acyl transferase (LCAT and ratio of sdLDL/cholesteryl ester transfer protein (CETP of controlled and uncontrolled T2DM. METHODS: This study was observational with cross sectional design. In total of 72 patients with T2DM consist of 36 controlled and 36 uncontrolled, participated in this study. The serum TG, HDL-C, sdLDL, LCAT and CETP were examined in their relationship with to T2DM risk. RESULTS: The results of the study indicate that the AIP (p<0.001 increase controlled and uncontrolled T2DM and the ratio of sdLDL/CETP (p=0.004, odds ratio of AIP was 4 (95% CI: 1.501-10.658 and odds ratio of sdLDL/CETP ratio was 4 (95% CI: 1.501-10.658 in uncontrolled T2DM. CONCLUSIONS: This study showed that the AIP and ratio of small dense LDL/CETP had a significant correlation with the uncontrolled T2DM. The AIP and ratio of small dense LDL/CETP increase was found at the uncontrolled T2DM to be 4 times greater than the controlled T2DM. KEYWORDS: T2DM, atherosclerosis, atherogenic index of plasma, small dense LDL, LCAT, CETP, ratio of sdLDL/LCAT, ratio of sdLDL/CETP.

  17. FINAL REPORT DM1200 TESTS WITH AZ 101 HLW SIMULANTS VSL-03R3800-4 REV 0 2/17/04

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; BARDAKCI T; D' ANGELO NA; GONG W; KOT WK; PEGG IL

    2011-12-29

    This report documents melter and off-gas performance results obtained on the DM 1200 HLW Pilot Melter during processing of simulated HLW AZ-101 feed. The principal objectives of the DM1200 melter testing were to determine the achievable glass production rates for simulated HLW AZ-101 feed; determine the effect of bubbling rate and feed solids content on production rate; characterize melter off-gas emissions; characterize the performance of the prototypical off-gas system components as well as their integrated performance; characterize the feed, glass product, and off-gas effluents; and to perform pre- and post-test inspections of system components. The test objectives (including test success criteria), along with how they were met, are outlined in a table.

  18. DichroMatch at the Protein Circular Dichroism Data Bank (DM@PCDDB): A Web-based Tool for Identifying Protein Nearest Neighbors using Circular Dichroism Spectroscopy.

    Science.gov (United States)

    Whitmore, Lee; Mavridis, Lazaros; Wallace, B A; Janes, Robert W

    2017-06-05

    Circular dichroism spectroscopy is a well-used, but simple method in structural biology for providing information on the secondary structure and folds of proteins. DichroMatch (DM@PCDDB) is an online tool that is newly available in the Protein Circular Dichroism Data Bank (PCDDB), which takes advantage of the wealth of spectral and metadata deposited therein, to enable identification of spectral nearest neighbours of a query protein based on six different methods of spectral matching. DM@PCDDB can potentially provide novel information about structural relationships between proteins and can be used in comparison studies of protein homologues and orthologues. This article is protected by copyright. All rights reserved. © 2017 The Protein Society.

  19. Topical or systemic 16,16 dm prostaglandin E sub 2 or WR-2721 (WR-1065) protects mice from alopecia after fractionated irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Geng, L.; Malkinson, F.D. (Rush-Presbyterian-St. Luke' s Medical Center, Chicago, IL (United States)); Hanson, W.R. (Hines VA Medical Center, IL (United States))

    1992-04-01

    Previous studies in mice demonstrated that systemic or topical 16,16 dm PGE{sub 2} protected against single dose radiation-induced hair loss. The authors have now investigated prostaglandin, or WR-2721, protection against murine alopecia produced by varying doses and schedules of fractionated radiation. In some studies with the radioprotectors given systematically, WR-2721 afforded slightly greater radioprotection than 16,16 dm PGE{sub 2}. The two compounds were essentially equally radioprotective in the topical application studies. Since both systemic and topical applications of the agents tested enhanced hair regrowth following radiation, the authors conclude that clinical use of these compounds may provide some protection of hair follicles, and perhaps other tissues, lying within a radiation therapy field. (author).

  20. The rationale, design, and baseline characteristics of PREVENT-DM: A community-based comparative effectiveness trial of lifestyle intervention and metformin among Latinas with prediabetes.

    Science.gov (United States)

    Perez, Alberly; Alos, Victor A; Scanlan, Adam; Maia, Catarina M; Davey, Adam; Whitaker, Robert C; Foster, Gary D; Ackermann, Ronald T; O'Brien, Matthew J

    2015-11-01

    Promotora Effectiveness Versus Metformin Trial (PREVENT-DM) is a randomized comparative effectiveness trial of a lifestyle intervention based on the Diabetes Prevention Program delivered by community health workers (or promotoras), metformin, and standard care. Eligibility criteria are Hispanic ethnicity, female sex, age ≥ 20 years, fluent Spanish-speaking status, BMI ≥ 23 kg/m(2), and prediabetes. We enrolled 92 participants and randomized them to one of the following three groups: standard care, DPP-based lifestyle intervention, or metformin. The primary outcome of the trial is the 12-month difference in weight between groups. Secondary outcomes include the following cardiometabolic markers: BMI, waist circumference, blood pressure, and fasting plasma glucose, hemoglobin A1C (HbA1c), total cholesterol, triglycerides, LDL cholesterol, HDL cholesterol, and insulin. PREVENT-DM participants are socioeconomically disadvantaged Latinas with a mean annual household income of $15,527 ± 9922 and educational attainment of 9.7 ± 3.6 years. Eighty-six percent of participants are foreign born, 20% have a prior history of gestational diabetes, and 71% have a first-degree relative with diagnosed diabetes. At baseline, PREVENT-DM participants had a mean age of 45.1 ± 12.5 years, weight of 178.8 ± 39.3 lbs, BMI of 33.3 ± 6.5 kg/m(2), HbA1c of 5.9 ± 0.2%, and waist circumference of 97.4 ± 11.1cm. Mean baseline levels of other cardiometabolic markers were normal. The PREVENT-DM study successfully recruited and randomized an understudied population of Latinas with prediabetes. This trial will be the first U.S. study to test the comparative effectiveness of metformin and lifestyle intervention versus standard care among prediabetic adults in a "real-world" setting.

  1. Angiosperms Are Unique among Land Plant Lineages in the Occurrence of Key Genes in the RNA-Directed DNA Methylation (RdDM) Pathway.

    Science.gov (United States)

    Ma, Lu; Hatlen, Andrea; Kelly, Laura J; Becher, Hannes; Wang, Wencai; Kovarik, Ales; Leitch, Ilia J; Leitch, Andrew R

    2015-09-02

    The RNA-directed DNA methylation (RdDM) pathway can be divided into three phases: 1) small interfering RNA biogenesis, 2) de novo methylation, and 3) chromatin modification. To determine the degree of conservation of this pathway we searched for key genes among land plants. We used OrthoMCL and the OrthoMCL Viridiplantae database to analyze proteomes of species in bryophytes, lycophytes, monilophytes, gymnosperms, and angiosperms. We also analyzed small RNA size categories and, in two gymnosperms, cytosine methylation in ribosomal DNA. Six proteins were restricted to angiosperms, these being NRPD4/NRPE4, RDM1, DMS3 (defective in meristem silencing 3), SHH1 (SAWADEE homeodomain homolog 1), KTF1, and SUVR2, although we failed to find the latter three proteins in Fritillaria persica, a species with a giant genome. Small RNAs of 24 nt in length were abundant only in angiosperms. Phylogenetic analyses of Dicer-like (DCL) proteins showed that DCL2 was restricted to seed plants, although it was absent in Gnetum gnemon and Welwitschia mirabilis. The data suggest that phases (1) and (2) of the RdDM pathway, described for model angiosperms, evolved with angiosperms. The absence of some features of RdDM in F. persica may be associated with its large genome. Phase (3) is probably the most conserved part of the pathway across land plants. DCL2, involved in virus defense and interaction with the canonical RdDM pathway to facilitate methylation of CHH, is absent outside seed plants. Its absence in G. gnemon, and W. mirabilis coupled with distinctive patterns of CHH methylation, suggest a secondary loss of DCL2 following the divergence of Gnetales. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  2. Association of Polymorphisms of β3—Adrenergic Receptor,Angiotensinogen and Angiotensin—Converting Enzyme Gene with Hypertension in type 2 DM

    Institute of Scientific and Technical Information of China (English)

    丁国宪; 沈捷; 等

    2002-01-01

    Objective To explore the relationship between the mutant genes of ACE,ATN,β3-AR and hypertension in patients with type 2DM.Methods 281 recruited Chinese subjects were divided into two groups according to the oral glucose tolerance test (OGTT):(1)non-diabetes group including normal and impaired glucose tolerance(NGT,IGT):169cases;(2)Type 2 diabetes mellitus(DM):112 cases.The subjects were genotyped for the ACE gene,the ATN gene and the codon 64 of β3-AR gene polymorphisms by applying polymerase chain reaction(PCR),PCR restriction fragment-length polymorphisms screening with the use of endonuclease.Results Our study found that the frequency of D/D genotype and D allele of ACE gene,a/a genotype and an allele of ATN gene in HT patients without DM were increased (P all<0.05);that the frequency of codon 64 mutation of β3-AR gene also increased in HT patients with NGT(P<0.05).In the model of multiple factors non-condition-al Logistic regression analyses,HT had relationship with history of hypertension,age and glucose tolerance(OR=10.7457,1.7804,2.0346;P=0.0004,0.0000,0.0246;respectively),with polymorphism of ATN gene,β3AR gene,ACE gene(OR=2.2736,1.9353,1.8309;P=0.0543,0.0287,0.0432;resceptively).Conclusion These results suggest that variants of ACE gene,β3AR gene,ATN gene were associated with HT in type 2 DM.

  3. Association of Polymorphisms of β3-Adrenergic Receptor,Angiotensinogen and Angiotensin-Converting Enzyme Gene with Hypertension in type 2 DM

    Institute of Scientific and Technical Information of China (English)

    丁国宪; 沈捷; 陈家伟

    2002-01-01

    ObjectiveTo explore the relationship between the mutant genes of ACE,ATN,β3-AR and hypertension in patients with type 2 DM.Methods281 recruited Chinese subjects were divided into two groups according to the mellitus (DM):112 cases.The subjects were genotyped for the ACE gene,the ATN gene and the codon 64 of β3-AR gene polymorphisms by applying polymerase chain reaction (PCR),PCR restriction fragment-length polymorphisms screening with the use of endonuclease.ResultsOur study found that the frequency of D/D genotype and D allele of ACE gene,a/a genotype and an allele of ATN gene in HT patients without DM were increased (P all <0.05);that the frequency of codon 64 mutation of β3-AR gene also increased in HT patients with NGT (P <0.05).In the model of multiple factors non-condition-al Logistic regression analyses,HT had relationship with history of hypertension,age and glucose tolerance (OR=10.745 7,1.780 4,2.034 6;P=0.000 4,0.000 0,0.024 6;respectively),with polymorphism of ATN gene,β3AR gene,ACE gene (OR=2.273 6,1.935 3,1.830 9;P=0.054 3,0.028 7,0.043 2;resceptively).ConclusionThese results suggest that variants of ACE gene,β3AR gene,ATN gene were associated with HT in type 2 DM.``

  4. Arabidopsis thaliana DM2h (R8) within the Landsberg RPP1-like Resistance Locus Underlies Three Different Cases of EDS1-Conditioned Autoimmunity.

    Science.gov (United States)

    Stuttmann, Johannes; Peine, Nora; Garcia, Ana V; Wagner, Christine; Choudhury, Sayan R; Wang, Yiming; James, Geo Velikkakam; Griebel, Thomas; Alcázar, Ruben; Tsuda, Kenichi; Schneeberger, Korbinian; Parker, Jane E

    2016-04-01

    Plants have a large panel of nucleotide-binding/leucine rich repeat (NLR) immune receptors which monitor host interference by diverse pathogen molecules (effectors) and trigger disease resistance pathways. NLR receptor systems are necessarily under tight control to mitigate the trade-off between induced defenses and growth. Hence, mis-regulated NLRs often cause autoimmunity associated with stunting and, in severe cases, necrosis. Nucleocytoplasmic ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) is indispensable for effector-triggered and autoimmune responses governed by a family of Toll-Interleukin1-Receptor-related NLR receptors (TNLs). EDS1 operates coincidently or immediately downstream of TNL activation to transcriptionally reprogram cells for defense. We show here that low levels of nuclear-enforced EDS1 are sufficient for pathogen resistance in Arabidopsis thaliana, without causing negative effects. Plants expressing higher nuclear EDS1 amounts have the genetic, phenotypic and transcriptional hallmarks of TNL autoimmunity. In a screen for genetic suppressors of nuclear EDS1 autoimmunity, we map multiple, independent mutations to one gene, DM2h, lying within the polymorphic DANGEROUS MIX2 cluster of TNL RPP1-like genes from A. thaliana accession Landsberg erecta (Ler). The DM2 locus is a known hotspot for deleterious epistatic interactions leading to immune-related incompatibilities between A. thaliana natural accessions. We find that DM2hLer underlies two further genetic incompatibilities involving the RPP1-likeLer locus and EDS1. We conclude that the DM2hLer TNL protein and nuclear EDS1 cooperate, directly or indirectly, to drive cells into an immune response at the expense of growth. A further conclusion is that regulating the available EDS1 nuclear pool is fundamental for maintaining homeostatic control of TNL immune pathways.

  5. Arabidopsis thaliana DM2h (R8 within the Landsberg RPP1-like Resistance Locus Underlies Three Different Cases of EDS1-Conditioned Autoimmunity.

    Directory of Open Access Journals (Sweden)

    Johannes Stuttmann

    2016-04-01

    Full Text Available Plants have a large panel of nucleotide-binding/leucine rich repeat (NLR immune receptors which monitor host interference by diverse pathogen molecules (effectors and trigger disease resistance pathways. NLR receptor systems are necessarily under tight control to mitigate the trade-off between induced defenses and growth. Hence, mis-regulated NLRs often cause autoimmunity associated with stunting and, in severe cases, necrosis. Nucleocytoplasmic ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1 is indispensable for effector-triggered and autoimmune responses governed by a family of Toll-Interleukin1-Receptor-related NLR receptors (TNLs. EDS1 operates coincidently or immediately downstream of TNL activation to transcriptionally reprogram cells for defense. We show here that low levels of nuclear-enforced EDS1 are sufficient for pathogen resistance in Arabidopsis thaliana, without causing negative effects. Plants expressing higher nuclear EDS1 amounts have the genetic, phenotypic and transcriptional hallmarks of TNL autoimmunity. In a screen for genetic suppressors of nuclear EDS1 autoimmunity, we map multiple, independent mutations to one gene, DM2h, lying within the polymorphic DANGEROUS MIX2 cluster of TNL RPP1-like genes from A. thaliana accession Landsberg erecta (Ler. The DM2 locus is a known hotspot for deleterious epistatic interactions leading to immune-related incompatibilities between A. thaliana natural accessions. We find that DM2hLer underlies two further genetic incompatibilities involving the RPP1-likeLer locus and EDS1. We conclude that the DM2hLer TNL protein and nuclear EDS1 cooperate, directly or indirectly, to drive cells into an immune response at the expense of growth. A further conclusion is that regulating the available EDS1 nuclear pool is fundamental for maintaining homeostatic control of TNL immune pathways.

  6. TNF-α is upregulated in T2DM patients with fracture and promotes the apoptosis of osteoblast cells in vitro in the presence of high glucose.

    Science.gov (United States)

    Sun, Mingqi; Yang, Junli; Wang, Jianzhong; Hao, Ting; Jiang, Dianming; Bao, Guoyu; Liu, Guanghui

    2016-04-01

    Fracture healing is regulated by proinflammatory mediators such as tumor necrosis factor-α (TNF-α), which poses influence on the balance between bone formation and remodeling. And the diabetes is thought to contribute to the delayed diabetic fracture healing. In the present study, we examined the promotion to proinflammatory cytokines and chemokines in type 2 diabetes mellitus (T2DM) patients with bone fractures, and then evaluated the promotion to TNF-α by the high glucose treatment in human osteoblast-like MG-63 cells and the regulatory role of the promoted TNF-α on the MG-63 cell apoptosis. It was demonstrated that there were significantly-upregulated high-sensitivity C-reactive protein (hsCRP) TNF-α, IL-1β, IL-6, IFN-γ-inducible protein 10 (IP-10) and RANTES in T2DM patients with bone fracture. And the promotion to TNF-α and IL-1β was confirmed in vitro in both mRNA and protein levels in high glucose-treated MG-63 cells. And either TNF-α or high glucose reduced the viability of MG-63 cells, promoted apoptosis and upregulated apoptosis-associated markers, such as released cytochrome c, cleaved caspase 3 and lyzed PARP. Moreover, there was a synergistic effect between TNF-α and high glucose. The viability reduction and the apoptosis induction of MG-63 cells were significantly higher in the group with both TNF-α and high glucose treatments, than in the group with singular TNF-α treatment. In conclusion, our study demonstrated that proinflammatory cytokines and chemokines were promoted in T2DM patients with bone fracture or in osteoblasts by the high glucose stimulation. TNF-α and high glucose synergistically reduced the viability and induced the apoptosis in the osteoblast-like MG-63 cells in vitro. It implies the significant regulatory role of TNF-α in the delayed fracture healing in T2DM.

  7. Effect of metformin combined with Liuwei Dihuang pill on serum illness-related molecule contents in T2DM patients with NAFLD

    Institute of Scientific and Technical Information of China (English)

    Zhong-Ping Zheng; Feng Tian; Zhi-Gang Zheng; Guo-Qing Zhang; Feng Guo; Xing-Hua Guo

    2016-01-01

    Objective:To study the effect of metformin combined with Liuwei Dihuang pill on serum illness-related molecule contents in T2DM patients with NAFLD.Methods: A total of 86 cases of newly diagnosed T2DM patients with NAFLD were selected for study and randomly divided into combination therapy group and monotherapy group who received metformin combined with Liuwei Dihuang pill therapy and metformin monotherapy respectively, and then serum glycolipid metabolism indexes, liver function indexes as well as oxidative stress response and inflammatory response indexes of two groups were detected.Results:(1) Glycolipid metabolism indexes: FPG, 2hPG, HbA1c, MAGE, HOMA-IR, TG, TC and LDL-C as well as BMI and waist hip ratio of combination therapy group were lower than those of monotherapy group, and HDL-C content was significantly higher than that of monotherapy group; (2) liver function indexes: AST, ALT,γ-GT, STB and CG contents of combination therapy group were significantly lower than those of monotherapy group; (3) oxidative stress response and inflammatory response indexes: MDA, AOPP, TNF-α and IL-6 contents of combination therapy group were significantly lower than those of monotherapy group.Conclusion:Metformin combined with Liuwei Dihuang pill helps to regulate glycolipid metabolism, reduce liver function damage and alleviate oxidative stress response and inflammatory response in T2DM patients with NAFLD.

  8. Potential probiotic attributes and antagonistic activity of an indigenous isolate Lactobacillus plantarum DM5 from an ethnic fermented beverage "Marcha" of north eastern Himalayas.

    Science.gov (United States)

    Das, Deeplina; Goyal, Arun

    2014-05-01

    A novel isolate DM5 identified as Lactobacillus plantarum displayed in vitro probiotic properties as well as antimicrobial activity. It showed adequate level of survival to the harsh conditions of the gastrointestinal tract and survived low acidic pH 2.5 for 5 h. Artificial gastric juice and intestinal fluidic environment decreased the initial viable cell population of isolate DM5 only by 7% and 13%, respectively, while lysozyme (200 µg/ml) and bile salt (0.5%) enhanced its growth. It was found to deconjugate taurodeoxycholic acid, indicating its potential to reduce hypercholesterolemia. Isolate DM5 demonstrated cell surface hydrophobicity of 53% and autoaggregation of 54% which are the prerequisite for adhesion to epithelial cells and colonization to host. Bacteriocin activity of isolate was found to be 6400 AU/ml as it inhibited the growth of food borne pathogens Escherichia coli, Staphylococcus aureus, and Alcaligenes faecalis. The bactericidal action of bacteriocin from isolate was analyzed by flow cytometry, rendering its use as prospective probiotic and starter culture in food industry.

  9. PI3K-GLUT4 Signal Pathway Associated with Effects of EX-B3 Electroacupuncture on Hyperglycemia and Insulin Resistance of T2DM Rats

    Directory of Open Access Journals (Sweden)

    Bing-Yan Cao

    2016-01-01

    Full Text Available Objectives. To explore electroacupuncture’s (EA’s effects on fasting blood glucose (FBG and insulin resistance of type 2 diabetic mellitus (T2DM model rats and give a possible explanation for the effects. Method. It takes high fat diet and intraperitoneal injection of streptozotocin (STZ, 30 mg/kg for model preparation. Model rats were randomly divided into T2DM Model group, EA weiwanxiashu (EX-B3 group, and sham EA group (n=12/group. EA (2 Hz continuous wave, 2 mA, 20 min/day, 6 days/week, 4 weeks was applied as intervention. FBG, area under curve (AUC of oral glucose tolerance test (OGTT, insulin resistance index (HOMA-IR, pancreatic B cell function index (HOMA-B, skeletal muscle phosphorylated phosphatidylinositol-3-kinase (PI3K, glucose transporter 4 (GLUT4, and membrane GLUT4 protein expression were measured. Results. EA weiwanxiashu (EX-B3 can greatly upregulate model rat’s significantly reduced skeletal muscle PI3K (Y607 and membrane GLUT4 protein expression (P<0.01, effectively reducing model rats’ FBG and AUC of OGTT (P<0.01. The effects are far superior to sham EA group. Conclusion. EA weiwanxiashu (EX-B3 can upregulate skeletal muscle phosphorylated PI3K protein expression, to stimulate membrane translocation of GLUT4 and thereby increase skeletal muscle glucose intake to treat T2DM.

  10. PI3K-GLUT4 Signal Pathway Associated with Effects of EX-B3 Electroacupuncture on Hyperglycemia and Insulin Resistance of T2DM Rats

    Science.gov (United States)

    2016-01-01

    Objectives. To explore electroacupuncture's (EA's) effects on fasting blood glucose (FBG) and insulin resistance of type 2 diabetic mellitus (T2DM) model rats and give a possible explanation for the effects. Method. It takes high fat diet and intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) for model preparation. Model rats were randomly divided into T2DM Model group, EA weiwanxiashu (EX-B3) group, and sham EA group (n = 12/group). EA (2 Hz continuous wave, 2 mA, 20 min/day, 6 days/week, 4 weeks) was applied as intervention. FBG, area under curve (AUC) of oral glucose tolerance test (OGTT), insulin resistance index (HOMA-IR), pancreatic B cell function index (HOMA-B), skeletal muscle phosphorylated phosphatidylinositol-3-kinase (PI3K), glucose transporter 4 (GLUT4), and membrane GLUT4 protein expression were measured. Results. EA weiwanxiashu (EX-B3) can greatly upregulate model rat's significantly reduced skeletal muscle PI3K (Y607) and membrane GLUT4 protein expression (P < 0.01), effectively reducing model rats' FBG and AUC of OGTT (P < 0.01). The effects are far superior to sham EA group. Conclusion. EA weiwanxiashu (EX-B3) can upregulate skeletal muscle phosphorylated PI3K protein expression, to stimulate membrane translocation of GLUT4 and thereby increase skeletal muscle glucose intake to treat T2DM. PMID:27656242

  11. Meta-Analysis of the association of IGF2BP2 gene rs4402960 polymorphisms with T2DM in Asia

    Directory of Open Access Journals (Sweden)

    Huang Zhengchun

    2017-01-01

    Full Text Available In order to evaluate the association of IGF2BP2 (SNPS:rs4402960 gene polymorphism and type 2 diabetes mellitus (T2DM susceptibility, we searched for all the related research literature by CNKI, Wanfan, Pubmed and Springer link database to collect data.Meta-analysis software(RevMan5.0 was applied for heterogeneity tests in genotype level, two methods(fixed-effects and random-effects modelwere performed to pool the odds ratio(OR. Random-effects model was adopted if there were obvious heterogeneity among studies. On the contrary, a fixed effect model was used. At the same time, publication bias was examined by Funnel plot. The studies collected 28 articles(including 35 studies, and included 52277cases of T2DM and 54168 controls. The pooled ORs of allele (T vs Gof rs4402960 polymorphic loci in IGF2BP2 was significant association with T2DM (OR=1.163 95%CI=[1.138,1.189] P<0.00001.

  12. Isolation and identification of the antagonistic strain DM-54 of Bacillus amyloliquefacien against Verticillium dahliae, and optimization of antifungal protein producing conditions

    Institute of Scientific and Technical Information of China (English)

    Ying ZHU; Shuna LI; Hongshui YUAN; Xiaojun GUO; Baocheng ZHU

    2009-01-01

    The strains capable of resistance against Verticillium dahliae Kleb were isolated and screened from the soils of cotton fields from several different provinces in China. A strain, coded DM-54, with a rather high antagonistic activity was obtained. Its morphological characteristics, physiological and biochemical properties and a 16 S rDNA sequence of this strain were further studied. The DM-54 strain was finally identified as a kind of Bacillus amyloliquefacien. Through a single factor experiment and an orthogonal experiment, the optimal shaking flask fermentation condition of strain DM-54 was found to be: media composed of 5% dextrin, 3% soy peptone, 0.02% MgSO4 0.01% CaCl2, initial pH 7.0 and 10% inoculum volume, media volume 30/250 (mL/mL), fermentation temperature at 32℃, rotating speed activity was distinguished to be elevated, at about 39.9%. Our research offers an effective means for the massive production of antagonistic proteins.

  13. Selective Detection and Automated Counting of Fluorescently-Labeled Chrysotile Asbestos Using a Dual-Mode High-Throughput Microscopy (DM-HTM Method

    Directory of Open Access Journals (Sweden)

    Jung Kyung Kim

    2013-05-01

    Full Text Available Phase contrast microscopy (PCM is a widely used analytical method for airborne asbestos, but it is unable to distinguish asbestos from non-asbestos fibers and requires time-consuming and laborious manual counting of fibers. Previously, we developed a high-throughput microscopy (HTM method that could greatly reduce human intervention and analysis time through automated image acquisition and counting of fibers. In this study, we designed a dual-mode HTM (DM-HTM device for the combined reflection and fluorescence imaging of asbestos, and automated a series of built-in image processing commands of ImageJ software to test its capabilities. We used DksA, a chrysotile-adhesive protein, for selective detection of chrysotile fibers in the mixed dust-free suspension of crysotile and amosite prepared in the laboratory. We demonstrate that fluorescently-stained chrysotile and total fibers can be identified and enumerated automatically in a high-throughput manner by the DM-HTM system. Combined with more advanced software that can correctly identify overlapping and branching fibers and distinguish between fibers and elongated dust particles, the DM-HTM method should enable fully automated counting of airborne asbestos.

  14. A study on the change and correlation of bone mineral density,vitamin D,IGF-I,IGF-II in T2DM male patients%T2DM 男性患者骨密度、IGF-I、IGF-II、维生素 D 的变化及相关性研究

    Institute of Scientific and Technical Information of China (English)

    张燕; 曾荣峰; 李延兵

    2015-01-01

    Objective To investigate the type 2 diabetes mellitus(T2DM)and bone mineral density(BMD) in male patients with insulin-like growth factor I(IGF-I),insulin-like growth factor II(IGF-II),the change and correlation of vitamin D.Methods One hundred cases of T2DM patients were chosen as T2DM group,50 cases of healthy controls undergoing physical examinations during the same period were chosen as control group and lumbar vertebral body(L2-4 )and femoral neck BMD,IGF-I,IGF-II and serum 25-hydroxy vitamin D3 were detected and correlation analysis was performed.Results In T2DM group,L2-4 and femoral neck BMD,IGF-I,IGF-II, 25-hydroxy vitamin D3 were lower than that of the control group( P <0.01).L2-4 bone mineral density and bone mineral density of fem-oral neck,IGF-I,IGF-II,25-hydroxy vitamin D3 between the two groups showed a positive correlation( P <0.01).Conclusion T2DM male patients may suffer from reduced bone mineral density,lower levels of IGF-I,IGF-II,vitamin D,and reduced IGF-I,IGF-II and vi-tamin D levels may be the relative factors causing decrease of BMD in T2DM male patients.%目的:探讨2型糖尿病(T2DM)男性患者骨密度、胰岛素样生长因子 I(IGF-I)、胰岛素样生长因子 II(IGF-II)及维生素 D 的变化及相关性。方法选取 T2DM 男性患者100例(T2DM 组),同期选取健康体检男性50例(对照组),分别检测两组腰椎体(L2~4)与股骨颈骨密度、IGF-I、IGF-II 及血清25-羟维生素 D3水平变化,分析其相关性。结果T2DM 组患者 L2~4与股骨颈骨密度、IGF-I、IGF-II、25-羟维生素 D3均低于对照组( P <0.01);L2~4骨密度、股骨颈骨密度、IGF-I、IGF-II、25-羟维生素 D3间两两比较均呈正相关性(均 P <0.01)。结论T2DM 男性患者存在骨密度、IGF-I、IGF-II 及维生素 D 水平降低, IGF-I、IGF-II、维生素 D 水平降低可能是引发 T2DM 男性患者骨密度降低的相关原因。

  15. 菌株DM8320安全评价及其发酵乳对大鼠肾结石形成的影响%Safety evaluation of Lactobacillus DM8320 and effects of its fermented milk on rat kidney stone formation

    Institute of Scientific and Technical Information of China (English)

    董婷婷; 朱琳; 袁杰利

    2013-01-01

    Objective To assess the safety of Lactobacillus DM8320 preliminarily and observe the effects of its fermented milk on rat kidney stone formation. Methods Acute toxicity test, bacterial translocation test and plasmid detection test were performed. In the model construction process of Wistar rats with kidney stones, fermented milk of DM8320 was administered. On the 29th day, the body weight and the contents of calcium and oxalic acid in urine were detected. Paraffin sections of rat left kidneys were made to observe the stone occurrence of each experimental group. Results DM8320 strains had no plasmids, no acute toxicity, and did not translocate in healthy rats. DM8320 fermented milk had reduced the oxalic acid and calcium contents in the urine of rats with kidney stones. Paraffin section results showed that the calcium oxalate content of the stone group was the largest, followed by the low dose treatment group, and that of the high dose treatment group was the least. Conclusion Strain DM8320 has a certain degree of safety, and its fermented milk can inhibit the formation of kidney stones in Wistar rats.%目的 对具有降解草酸能力的菌株DM8320进行初步安全性评价并观察其发酵乳对肾结石模型大鼠的影响.方法 通过检测DM8320的质粒,细菌易位试验以及急性毒性试验对DM8320进行初步安全性评价.构建大鼠肾结石模型的同时灌胃目的菌株的牛奶发酵乳,实验第29天,收集24 h尿液并检测尿液中钙离子、草酸的含量.取大鼠左肾制作石蜡切片,观察各个实验组结石发生情况.结果 菌株DM8320中不含质粒,未发生细菌易位现象,不具有毒性.DM8320发酵乳可降低肾结石模型大鼠尿中的草酸和钙离子含量.观察各组大鼠肾脏石蜡切片,结石组中结石含量最多,低剂量组次之,高剂量组肾结石结晶含量较少.结论 菌株DM8320具有一定的安全性,其发酵乳对Wistar大鼠肾结石的形成具有一定的抑制作用.

  16. Protection against T1DM-Induced Bone Loss by Zinc Supplementation: Biomechanical, Histomorphometric, and Molecular Analyses in STZ-Induced Diabetic Rats.

    Directory of Open Access Journals (Sweden)

    Raul Hernandes Bortolin

    Full Text Available Several studies have established an association between diabetes and alterations in bone metabolism; however, the underlying mechanism is not well established. Although zinc is recognized as a potential preventive agent against diabetes-induced bone loss, there is no evidence demonstrating its effect in chronic diabetic conditions. This study evaluated the effects of zinc supplementation in a chronic (90 days type 1 diabetes-induced bone-loss model. Male Wistar rats were distributed in three groups: control, type 1 diabetes mellitus (T1DM, and T1DM plus zinc supplementation (T1DMS. Serum biochemical analysis; tibia histomorphometric, biomechanical, and collagen-content analyses; and femur mRNA expression were evaluated. Relative to T1DM, the zinc-supplemented group showed increased histomorphometric parameters such as TbWi and BAr and decreased TbSp, increased biomechanical parameters (maximum load, stiffness, ultimate strain, and Young's modulus, and increased type I collagen content. Interestingly, similar values for these parameters were observed between the T1DMS and control groups. These results demonstrate the protective effect of zinc on the maintenance of bone strength and flexibility. In addition, downregulation of OPG, COL1A, and MMP-9 genes was observed in T1DMS, and the anabolic effects of zinc were evidenced by increased OC expression and serum ALP activity, both related to osteoblastogenesis, demonstrating a positive effect on bone formation. In contrast, T1DM showed excessive bone loss, observed through reduced histomorphometric and biomechanical parameters, characterizing diabetes-associated bone loss. The bone loss was also observed through upregulation of OPG, COL1A, and MMP-9 genes. In conclusion, zinc showed a positive effect on the maintenance of bone architecture and biomechanical parameters. Indeed, OC upregulation and control of expression of OPG, COL1A, and MMP-9 mRNAs, even in chronic hyperglycemia, support an anabolic

  17. Protection against T1DM-Induced Bone Loss by Zinc Supplementation: Biomechanical, Histomorphometric, and Molecular Analyses in STZ-Induced Diabetic Rats.

    Science.gov (United States)

    Bortolin, Raul Hernandes; da Graça Azevedo Abreu, Bento João; Abbott Galvão Ururahy, Marcela; Costa de Souza, Karla Simone; Bezerra, João Felipe; Loureiro, Melina Bezerra; da Silva, Flávio Santos; Marques, Dáfiny Emanuele da Silva; Batista, Angélica Amanda de Sousa; Oliveira, Gisele; Luchessi, André Ducati; Lima, Valéria Morgiana Gualberto Duarte Moreira; Miranda, Carlos Eduardo Saraiva; Lia Fook, Marcus Vinicius; Almeida, Maria das Graças; de Rezende, Luciana Augusto; de Rezende, Adriana Augusto

    2015-01-01

    Several studies have established an association between diabetes and alterations in bone metabolism; however, the underlying mechanism is not well established. Although zinc is recognized as a potential preventive agent against diabetes-induced bone loss, there is no evidence demonstrating its effect in chronic diabetic conditions. This study evaluated the effects of zinc supplementation in a chronic (90 days) type 1 diabetes-induced bone-loss model. Male Wistar rats were distributed in three groups: control, type 1 diabetes mellitus (T1DM), and T1DM plus zinc supplementation (T1DMS). Serum biochemical analysis; tibia histomorphometric, biomechanical, and collagen-content analyses; and femur mRNA expression were evaluated. Relative to T1DM, the zinc-supplemented group showed increased histomorphometric parameters such as TbWi and BAr and decreased TbSp, increased biomechanical parameters (maximum load, stiffness, ultimate strain, and Young's modulus), and increased type I collagen content. Interestingly, similar values for these parameters were observed between the T1DMS and control groups. These results demonstrate the protective effect of zinc on the maintenance of bone strength and flexibility. In addition, downregulation of OPG, COL1A, and MMP-9 genes was observed in T1DMS, and the anabolic effects of zinc were evidenced by increased OC expression and serum ALP activity, both related to osteoblastogenesis, demonstrating a positive effect on bone formation. In contrast, T1DM showed excessive bone loss, observed through reduced histomorphometric and biomechanical parameters, characterizing diabetes-associated bone loss. The bone loss was also observed through upregulation of OPG, COL1A, and MMP-9 genes. In conclusion, zinc showed a positive effect on the maintenance of bone architecture and biomechanical parameters. Indeed, OC upregulation and control of expression of OPG, COL1A, and MMP-9 mRNAs, even in chronic hyperglycemia, support an anabolic and protective

  18. FINAL REPORT SUMMARY OF DM 1200 OPERATION AT VSL VSL-06R6710-2 REV 0 9/7/06

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; DIENER G; BARDAKCI T; PEGG IL

    2011-12-29

    The principal objective of this report was to summarize the testing experience on the DuraMelter 1200 (DMI200), which is the High Level Waste (HLW) Pilot Melter located at the Vitreous State Laboratory (VSL). Further objectives were to provide descriptions of the history of all modifications and maintenance, methods of operation, problems and unit failures, and melter emissions and performance while processing a variety of simulated HL W and low activity waste (LAW) feeds for the Hanford Waste Treatment and Immobilization Plant (WTP) and employing a variety of operating methods. All of these objectives were met. The River Protection Project - Hanford Waste Treatment and Immobilization Plant (RPP-WTP) Project has undertaken a 'tiered' approach to vitrification development testing involving computer-based glass formulation, glass property-composition models, crucible melts, and continuous melter tests of increasing, more realistic scales. Melter systems ranging from 0.02 to 1.2 m{sup 2} installed at the Vitreous State Laboratory (VSL) have been used for this purpose, which, in combination with the 3.3 m{sup 2} low activity waste (LAW) Pilot Melter at Duratek, Inc., span more than two orders of magnitude in melt surface area. In this way, less-costly small-scale tests can be used to define the most appropriate tests to be conducted at the larger scales in order to extract maximum benefit from the large-scale tests. For high level waste (HLW) vitrification development, a key component in this approach is the one-third scale DuraMelter 1200 (DM 1200), which is the HLW Pilot Melter that has been installed at VSL with an integrated prototypical off-gas treatment system. That system replaced the DM1000 system that was used for HLW throughput testing during Part B1. Both melters have similar melt surface areas (1.2 m{sup 2}) but the DM1200 is prototypical of the present RPP-WTP HLW melter design whereas the DM1000 was not. In particular, the DM1200 provides for

  19. DS-8201a, a new HER2-targeting antibody-drug conjugate incorporating a novel DNA topoisomerase I inhibitor, overcomes HER2-positive gastric cancer T-DM1 resistance.

    Science.gov (United States)

    Takegawa, Naoki; Nonagase, Yoshikane; Yonesaka, Kimio; Sakai, Kazuko; Maenishi, Osamu; Ogitani, Yusuke; Tamura, Takao; Nishio, Kazuto; Nakagawa, Kazuhiko; Tsurutani, Junji

    2017-10-15

    Anti-HER2 therapies are beneficial for patients with HER2-positive breast or gastric cancer. T-DM1 is a HER2-targeting antibody-drug conjugate (ADC) comprising the antibody trastuzumab, a linker, and the tubulin inhibitor DM1. Although effective in treating advanced breast cancer, all patients eventually develop T-DM1 resistance. DS-8201a is a new ADC incorporating an anti-HER2 antibody, a newly developed, enzymatically cleavable peptide linker, and a novel, potent, exatecan-derivative topoisomerase I inhibitor (DXd). DS-8201a has a drug-to-antibody-ratio (DAR) of 8, which is higher than that of T-DM1 (3.5). Owing to these unique characteristics and unlike T-DM1, DS-8201a is effective against cancers with low-HER2 expression. In the present work, T-DM1-resistant cells (N87-TDMR), established using the HER2-positive gastric cancer line NCI-N87 and continuous T-DM1 exposure, were shown to be susceptible to DS-8201a. The ATP-binding cassette (ABC) transporters ABCC2 and ABCG2 were upregulated in N87-TDMR cells, but HER2 overexpression was retained. Furthermore, inhibition of ABCC2 and ABCG2 by MK571 restored T-DM1 sensitivity. Therefore, resistance to T-DM1 is caused by efflux of its payload DM1, due to aberrant expression of ABC transporters. In contrast to DM1, DXd payload of DS-8201a inhibited the growth of N87-TDMR cells in vitro. This suggests that either DXd may be a poor substrate of ABCC2 and ABCG2 in comparison to DM1, or the high DAR of DS-8201a relative to T-DM1 compensates for increased efflux. Notably, N87-TDMR xenograft tumor growth was prevented by DS-8201a. In conclusion, the efficacy of DS-8201a as a treatment for patients with T-DM1-resistant breast or gastric cancer merits investigation. © 2017 UICC.

  20. A khorasan wheat-based replacement diet improves risk profile of patients with type 2 diabetes mellitus (T2DM): a randomized crossover trial.

    Science.gov (United States)

    Whittaker, Anne; Dinu, Monica; Cesari, Francesca; Gori, Anna Maria; Fiorillo, Claudia; Becatti, Matteo; Casini, Alessandro; Marcucci, Rossella; Benedettelli, Stefano; Sofi, Francesco

    2017-04-01

    The aim of the present study was to examine whether a replacement diet with products made with organic ancient khorasan wheat could provide additive protective effects in reducing glucose, insulin, lipid and inflammatory risk factors, and in restoring blood redox balance in type 2 diabetes mellitus (T2DM) patients compared to diet with product made with modern organic wheat. We conducted a randomized, double-blinded crossover trial with two intervention phases on 21 T2DM patients (14 females, 7 males). The participants were assigned to consume products (bread, pasta, crackers and biscuits) made using semi-whole flour from organic wheat that was either from ancient khorasan wheat or modern control wheat for 8 weeks in a random order. An 8-week washout period was implemented between the interventions. Laboratory analyses were performed both at the beginning and at the end of each intervention phase. The metabolic risk profile improved only after the khorasan intervention period, as measured by a reduction in total and LDL cholesterol (mean reduction: -3.7 and -3.4 %, respectively), insulin (-16.3 %) and blood glucose (-9.1 %). Similarly, there was a significant reduction in circulating levels of reactive oxygen species (ROS), vascular endothelial growth factor (VEGF) and interleukin-1ra, and a significant increase of total antioxidant capacity (+6.3 %). No significant differences from baseline were noted after the modern control wheat intervention phase. The change (from pre- to post-intervention) between the two intervention arms was significantly different (p diet with ancient khorasan wheat consumption provided additive protection in reducing total and LDL cholesterol, insulin, blood glucose, ROS production, and some inflammatory risk factors, which are all key factors warranting of control in secondary prevention of T2DM compared to a diet with products made with modern wheat.

  1. Computer modelling in combination with in vitro studies reveals similar binding affinities of Drosophila Crumbs for the PDZ domains of Stardust and DmPar-6.

    Science.gov (United States)

    Kempkens, Ozlem; Médina, Emmanuelle; Fernandez-Ballester, Gregorio; Ozüyaman, Susann; Le Bivic, André; Serrano, Luis; Knust, Elisabeth

    2006-08-01

    Formation of multiprotein complexes is a common theme to pattern a cell, thereby generating spatially and functionally distinct entities at specialised regions. Central components of these complexes are scaffold proteins, which contain several protein-protein interaction domains and provide a platform to recruit a variety of additional components. There is increasing evidence that protein complexes are dynamic structures and that their components can undergo various interactions depending on the cellular context. However, little is known so far about the factors regulating this behaviour. One evolutionarily conserved protein complex, which can be found both in Drosophila and mammalian epithelial cells, is composed of the transmembrane protein Crumbs/Crb3 and the scaffolding proteins Stardust/Pals1 and DPATJ/PATJ, respectively, and localises apically to the zonula adherens. Here we show by in vitro analysis that, similar as in vertebrates, the single PDZ domain of Drosophila DmPar-6 can bind to the four C-terminal amino acids (ERLI) of the transmembrane protein Crumbs. To further evaluate the binding capability of Crumbs to DmPar-6 and the MAGUK protein Stardust, analysis of the PDZ structural database and modelling of the interactions between the C-terminus of Crumbs and the PDZ domains of these two proteins were performed. The results suggest that both PDZ domains bind Crumbs with similar affinities. These data are supported by quantitative yeast two-hybrid interactions. In vivo analysis performed in cell cultures and in the Drosophila embryo show that the cytoplasmic domain of Crumbs can recruit DmPar-6 and DaPKC to the plasma membrane. The data presented here are discussed with respect to possible dynamic interactions between these proteins.

  2. PARTICIPATION OF Y89 AND Y97 IN THE CONJUGATING ACTIVITY OF Drosophila melanogaster GLUTATHIONE S-TRANSFERASE D3 (DmGSTD3).

    Science.gov (United States)

    Vignesvaran, Kithalakshmi; Alias, Zazali

    2016-07-01

    Drosophila melanogaster glutathione S-transferase D3 (DmGSTD3) has a shorter amino acid sequence as compared to other GSTs known in the fruit flies. This is due to the 15 amino acid N-terminal truncation in which normally active amino acid residue is located. The work has made use of homology modeling to visualize the arrangement of amino acid side chains in the glutathione (GSH) substrate cavity. The identified amino acids were then replaced with amino acids without functional groups in the side chains and the mutants were analyzed kinetically. Homology modeling revealed that the side chains of Y89 and Y97 were shown facing toward the substrate cavity proposing their possible role in catalyzing the conjugation. Y97A and Y89A GSH gave large changes in Km (twofold increase), Vmax (fivefold reduction), and Kcat /Km values for GSH suggesting their significant role in the conjugation reaction. The replacement at either positions has not affected the affinity of the enzyme toward 1-chloro-2,4-dinitrobenzene as no significant change in values of Kmax was observed. The replacement, however, had significantly reduced the catalytic efficiency of both mutants with (Kcat /Km )(GSH) and (Kcat /Km )(CDNB) of eight- and twofold reduction. The recombinant DmGSTD3 has shown no activity toward 1,2-dichloro-4-nitrobenzene, 2,4-hexadienal, 2,4-heptadienal, p-nitrobenzyl chloride, ethacrynic acid, and sulfobromophthalein. Therefore, it was evident that DmGSTD3 has made use of distal amino acids Y97 and Y89 for GSH conjugation.

  3. TECHNETIUM RETENTION IN WTP LAW GLASS WITH RECYCLE FLOW-SHEET DM10 MELTER TESTING VSL-12R2640-1 REV 0

    Energy Technology Data Exchange (ETDEWEB)

    Abramowitz, Howard [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; Brandys, Marek [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; Cecil, Richard [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; D& #x27; Angelo, Nicholas [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; Matlack, Keith S. [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; Muller, Isabelle S. [Catholic Univ. of America, Washington, DC (United States). Vitreous State Lab.; Pegg, Ian L. [Energy Solutions, Federal EPC, Inc., Columbia, MD (United States); Callow, Richard A. [Energy Solutions, Federal EPC, Inc., Columbia, MD (United States); Joseph, Innocent

    2012-12-11

    Melter tests were conducted to determine the retention of technetium and other volatiles in glass while processing simulated Low Activity Waste (LAW) streams through a DM10 melter equipped with a prototypical off-gas system that concentrates and recycles fluid effiuents back to the melter feed. To support these tests, an existing DM10 system installed at Vitreous State Laboratory (VSL) was modified to add the required recycle loop. Based on the Hanford Tank Waste Treatment and Immobilization Plant (WTP) LAW off-gas system design, suitably scaled versions of the Submerged Bed Scrubber (SBS), Wet Electrostatic Precipitator (WESP), and TLP vacuum evaporator were designed, built, and installed into the DM10 system. Process modeling was used to support this design effort and to ensure that issues associated with the short half life of the {sup 99m}Tc radioisotope that was used in this work were properly addressed and that the system would be capable of meeting the test objectives. In particular, this required that the overall time constant for the system was sufficiently short that a reasonable approach to steady state could be achieved before the {sup 99m}Tc activity dropped below the analytical limits of detection. The conceptual design, detailed design, flow sheet development, process model development, Piping and Instrumentation Diagram (P&ID) development, control system design, software design and development, system fabrication, installation, procedure development, operator training, and Test Plan development for the new system were all conducted during this project. The new system was commissioned and subjected to a series of shake-down tests before embarking on the planned test program. Various system performance issues that arose during testing were addressed through a series of modifications in order to improve the performance and reliability of the system. The resulting system provided a robust and reliable platform to address the test objectives.

  4. Resolution of Pd catalyst with tropos biphenylphosphine (BIPHEP) ligand by DM-DABN: asymmetric catalysis by an enantiopure BIPHEP-Pd complex.

    Science.gov (United States)

    Mikami, Koichi; Aikawa, Kohsuke; Yusa, Yukinori; Hatano, Manabu

    2002-01-10

    [reaction: see text] The racemic Pd complex with the chirally flexible (tropos) biphenylphosphine (BIPHEP) ligand can be resolved with enantiopure 3,3'-dimethyl-2,2'-diamino-1,1'-binaphthyl (DM-DABN) as a resolving agent at room temperature. The enantiopure BIPHEP-Pd complex is obtained from complexation with enantiopure DABN followed by tropo-inversion into the single BIPHEP-Pd diastereomer at 80 degrees C and protonation at 0 degrees C. The enantiopure BIPHEP-Pd complex can be used as an efficient Lewis acid catalyst for the Diels-Alder reaction at room temperature to give high enantioselectivity (82% ee, 60%).

  5. DS-8201a, A Novel HER2-Targeting ADC with a Novel DNA Topoisomerase I Inhibitor, Demonstrates a Promising Antitumor Efficacy with Differentiation from T-DM1.

    Science.gov (United States)

    Ogitani, Yusuke; Aida, Tetsuo; Hagihara, Katsunobu; Yamaguchi, Junko; Ishii, Chiaki; Harada, Naoya; Soma, Masako; Okamoto, Hiromi; Oitate, Masataka; Arakawa, Shingo; Hirai, Takehiro; Atsumi, Ryo; Nakada, Takashi; Hayakawa, Ichiro; Abe, Yuki; Agatsuma, Toshinori

    2016-10-15

    An anti-HER2 antibody-drug conjugate with a novel topoisomerase I inhibitor, DS-8201a, was generated as a new antitumor drug candidate, and its preclinical pharmacologic profile was assessed. In vitro and in vivo pharmacologic activities of DS-8201a were evaluated and compared with T-DM1 in several HER2-positive cell lines and patient-derived xenograft (PDX) models. The mechanism of action for the efficacy was also evaluated. Pharmacokinetics in cynomolgus monkeys and the safety profiles in rats and cynomolgus monkeys were assessed. DS-8201a exhibited a HER2 expression-dependent cell growth-inhibitory activity and induced tumor regression with a single dosing at more than 1 mg/kg in a HER2-positive gastric cancer NCI-N87 model. Binding activity to HER2 and ADCC activity of DS-8201a were comparable with unconjugated anti-HER2 antibody. DS-8201a also showed an inhibitory activity to Akt phosphorylation. DS-8201a induced phosphorylation of Chk1 and Histone H2A.X, the markers of DNA damage. Pharmacokinetics and safety profiles of DS-8201a were favorable and the highest non-severely toxic dose was 30 mg/kg in cynomolgus monkeys, supporting DS-8201a as being well tolerated in humans. DS-8201a was effective in a T-DM1-insensitive PDX model with high HER2 expression. DS-8201a, but not T-DM1, demonstrated antitumor efficacy against several breast cancer PDX models with low HER2 expression. DS-8201a exhibited a potent antitumor activity in a broad selection of HER2-positive models and favorable pharmacokinetics and safety profiles. The results demonstrate that DS-8201a will be a valuable therapy with a great potential to respond to T-DM1-insensitive HER2-positive cancers and low HER2-expressing cancers. Clin Cancer Res; 22(20); 5097-108. ©2016 AACR. ©2016 American Association for Cancer Research.

  6. INFLUENCIA DE LAS TÉCNICAS DE RELAJACIÓN Y MANEJO DEL ESTRÉS EN PACIENTES CON DM2 E HIPERTENSIÓN ARTERIAL.

    OpenAIRE

    Rodríguez Sevillano, Brendha Liliana

    2012-01-01

    NTRODUCCION: La diabetes mellitus e hipertensión arterial, con frecuencia se acompañan de estrés. Las técnicas de relajación ayudan a disminuir el estrés. OBJETIVO: Identificar cambios en el estilo de vida y respuesta al estrés de pacientes con DM2 e HAS, después de la practica de técnicas de relajación y manejo del estrés. Cuasi- experimental. Se incluyeron 15 MATERIAL Y METODOS: estudio pacientes comparados con un grupo de pacientes que no recibieron técnicas de rela...

  7. Macro-scale complexity of nano- to micro-scale architecture of olivine crystals through an iodine vapour transport mechanism

    Indian Academy of Sciences (India)

    Raymond L D Whitby; Takahiro Fukuda; Toru Maekawa

    2014-04-01

    The production of nano- to micro-scale olivine (magnesium and iron silicate) crystals has been achieved at relatively low temperatures through an iodine vapour transport of the metal onto amorphous silicon dioxide. The process occurs down a temperature gradient from 800 to 600°C yielding high quality crystals with long range crystallinity, highly complex interconnectivity and intricate macroscale architecture. Scanning electron microscopy (SEM) imaging of the substrate before and after the reaction reveals that the amorphous silicon oxide species is mobile, due to the lack of correlation between the silicon oxide layer and the final olivine particles, leading to a vapour–liquid–solid or vapour–solid growth mechanism. This technique demonstrates a facile, low temperature synthetic route towards olivine crystals with nano- to micro-scale dimensions.

  8. A Spatially Explicit and Seasonally Varying Cholera Prevalence Model With Distributed Macro-Scale Environmental and Hydroclimatic Forcings

    Science.gov (United States)

    Akanda, A. S.; Jutla, A. S.; Eltahir, E. A.; Islam, S.

    2011-12-01

    Despite major advances in the ecological and microbiological understanding of the bacterium Vibrio cholerae, the role of underlying large-scale processes in the progression of the cholera disease in space and time is not well understood. Here, we present a spatially explicit and seasonally varying coupled hydroclimatology-epidemiology model for understanding regional scale cholera prevalence in response to large scale hydroclimatic and environmental forcings. Our results show that environmental cholera transmission can be modulated by two spatially and seasonally distinct mechanisms - influenced by dry and wet season hydrologic determinants. The model is applied to the Ganges-Brahmaputra-Meghna Basin areas in Bangladesh to simulate spatially explicit cholera prevalence rates, and validated with long-term cholera data from Dhaka and shorter-term records from regional surveillance locations. The model reproduces the variability of cholera prevalence at monthly, seasonal, and interannual timescales and highlights the role of asymmetric large scale hydroclimatic processes as the dominant controls. Our findings have important implications for formulating effective cholera intervention strategies, and for understanding the impacts of changing climate patterns on seasonal cholera transmission.

  9. From macro-scale to micro-scale computational anatomy: a perspective on the next 20 years.

    Science.gov (United States)

    Mori, Kensaku

    2016-10-01

    This paper gives our perspective on the next two decades of computational anatomy, which has made great strides in the recognition and understanding of human anatomy from conventional clinical images. The results from this field are now used in a variety of medical applications, including quantitative analysis of organ shapes, interventional assistance, surgical navigation, and population analysis. Several anatomical models have also been used in computational anatomy, and these mainly target millimeter-scale shapes. For example, liver-shape models are almost completely modeled at the millimeter scale, and shape variations are described at such scales. Most clinical 3D scanning devices have had just under 1 or 0.5 mm per voxel resolution for over 25 years, and this resolution has not changed drastically in that time. Although Z-axis (head-to-tail direction) resolution has been drastically improved by the introduction of multi-detector CT scanning devices, in-plane resolutions have not changed very much either. When we look at human anatomy, we can see different anatomical structures at different scales. For example, pulmonary blood vessels and lung lobes can be observed in millimeter-scale images. If we take 10-µm-scale images of a lung specimen, the alveoli and bronchiole regions can be located in them. Most work in millimeter-scale computational anatomy has been done by the medical-image analysis community. In the next two decades, we encourage our community to focus on micro-scale computational anatomy. In this perspective paper, we briefly review the achievements of computational anatomy and its impacts on clinical applications; furthermore, we show several possibilities from the viewpoint of microscopic computational anatomy by discussing experimental results from our recent research activities.

  10. Meso-Scale Experimental & Numerical Studies for Predicting Macro-scale Performance of Advanced Reactive Materials (ARMs)

    Science.gov (United States)

    2015-04-01

    develop and implement the techniques to quantify and mathematically represent such differences in the spatial arrangements; to formulate approaches to...two-point correlation functions are mathematically constrained to be equal at 0, the t-test fails to reject the hypothesis that the two functions are...2002). 8 S.H. Fisher and M.C. Grubelich, 32ndAIAA/ASME/SAE/ASEE Jointt Propulsion conference, Lake Buena Vista , FL, July 1-3, 1996, SANDIA Report

  11. Experimental Investigation on Effect of Fin Height on Microscale Heat Transfer and Fluid Flow for Macro Scale Industrial Applications

    Science.gov (United States)

    Cheng, K. X.; Goh, A. L.; Hadi, M.; Ooi, K. T.

    2017-03-01

    Microchannel for macro geometry application is gaining popularity particularly in aerospace, biomedical and photovoltaic. A novel method of employing microchannel in macro geometry at lower cost using conventional machining methods has been developed. A solid cylinder on outer diameter 19.4 mm is placed concentrically into a copper pipe of inner diameter 20 mm, forming an annular microchannel with 300 μm gap. This study takes a step further by introducing surface profile of different heights on the surface of solid cylinder and investigating the effect on two main design objectives- increasing heat removal capability at same pumping power and reducing pumping power for the same heat removal duty. Four surface profiles -parallel fins as well as fins with height of 0.1, 0.2 and 0.3 mm, were investigated experimentally at constant heat flux at Reynolds number from 690 to 4600. The amount of fluid in the microchannel, channel length of 30 mm, bifurcating angle of 75 degrees and mean hydraulic diameter of 600 μm are kept as constant parameters. A plain insert is used as benchmark for comparison of enhancement. In this study, insert with fins of 0.3 mm attains the highest enhancement of 43 percent increment in heat transfer as compared to plain insert using the same pumping power. While keeping the heat removal duty constant, the same insert is able to perform the duty using less than 50 percent the pumping power required by the plain insert at low Reynolds numbers.

  12. Meso-Scale and Macro-Scale Analysis of the Geochemical and Physical Processes Responsible for Acid Rock Drainage

    Science.gov (United States)

    Otwinowski, Matthew

    1997-08-01

    We have developed a computer model which describes the geochemical and physical processes responsible for acid drainage from waste rock piles. The model is in the form of coupled nonlinear PDEs which describe: the kinetics of the chemical reactions, the release of contaminants, the generation of energy due to the exothermic oxidation of sulphides, the diffusive and convective transport of oxygen and water, and the transport of energy by conduction and convection. The meso-scale and large-scale characterization of waste rock and waste rock piles is discussed. We show that long-term leaching rates are inversely proportional to the square of particle diameter and that the previously used models underestimate the particle size effect on long-term sulphide oxidation. Experimental data on rock fragmentation are used for a fractal statistical characterization of waste rock piles. The acid generation rates, oxygen consumption rates and temperature profiles have been determined for piles containing from fifty thousand to five hundred thousand tonnes of waste rock. The thermodynamic instabilities, which occur at certain critical values of pile height, are responsible for thermodynamic catastrophes which result in a rapid increase of acid generation rates. The critical height is determined by the values of sulphide concentration, particle size, pile porosity and other factors. The numerical code is based on the finite elements method with an adaptive grid generator. abstract.

  13. Damage Prediction Using Several Types of Macro-scale Damage Models in Different Cold Wire Production Lines

    OpenAIRE

    Cao, Trong-Son; Montmitonnet, Pierre; Bouchard, Pierre-Olivier; Bobadilla, Christian; Vachey, Christophe

    2014-01-01

    International audience; The purpose of the present paper is to show how and to what extent the introduction of refined, shear sensitive models improves on previous ones, based on triaxiality only, for the phenomenological description of ductile damage in bulk cold metal forming processes. Wire-drawing and wire rolling are taken as examples. A set of mechanical tests has been conducted: round bar tension, notched bar tension, plane strain tension, and torsion for pure shear deformation. Both c...

  14. Modelling PM10 aerosol data from the Qalabotjha low-smoke fuels macro-scale experiment in South Africa

    CSIR Research Space (South Africa)

    Engelbrecht, JP

    2000-03-30

    Full Text Available D-grade (i.e. poor quality) coal is widely used for household cooking and heating purposes by lower-income urban communities in South Africa. The smoke from the combustion of coal has had a severe impact on the health of society in the townships...

  15. Glaciers and small ice caps in the macro-scale hydrological cycle - an assessment of present conditions and future changes

    Science.gov (United States)

    Lammers, Richard; Hock, Regine; Prusevich, Alexander; Bliss, Andrew; Radic, Valentina; Glidden, Stanley; Grogan, Danielle; Frolking, Steve

    2014-05-01

    Glacier and small ice cap melt water contributions to the global hydrologic cycle are an important component of human water supply and for sea level rise. This melt water is used in many arid and semi-arid parts of the world for direct human consumption as well as indirect consumption by irrigation for crops, serving as frozen reservoirs of water that supplement runoff during warm and dry periods of summer when it is needed the most. Additionally, this melt water reaching the oceans represents a direct input to sea level rise and therefore accurate estimates of this contribution have profound economic and geopolitical implications. It has been demonstrated that, on the scale of glacierized river catchments, land surface hydrological models can successfully simulate glacier contribution to streamflow. However, at global scales, the implementation of glacier melt in hydrological models has been rudimentary or non-existent. In this study, a global glacier mass balance model is coupled with the University of New Hampshire Water Balance/Transport Model (WBM) to assess recent and projected future glacier contributions to the hydrological cycle over the global land surface (excluding the ice sheets of Greenland and Antarctica). For instance, results of WBM simulations indicate that seasonal glacier melt water in many arid climate watersheds comprises 40 % or more of their discharge. Implicitly coupled glacier and WBM models compute monthly glacier mass changes and resulting runoff at the glacier terminus for each individual glacier from the globally complete Randolph Glacier Inventory including over 200 000 glaciers. The time series of glacier runoff is aggregated over each hydrological modeling unit and delivered to the hydrological model for routing downstream and mixing with non-glacial contribution of runoff to each drainage basin outlet. WBM tracks and uses glacial and non-glacial components of the in-stream water for filling reservoirs, transfers of water between drainage basins (inter-basin hydrological transfers), and irrigation along the global system of rivers with net discharge to the ocean. Climate scenarios from global climate models prepared for IPCC AR5 are used to explore an expected range of possible future glacier outflow variability to estimate the impacts on human use of these valuable waters and their poorly understood net contribution to sea level change.

  16. FINAL REPORT REGULATORY OFF GAS EMISSIONS TESTING ON THE DM1200 MELTER SYSTEM USING HLW AND LAW SIMULANTS VSL-05R5830-1 REV 0 10/31/05

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; BRANDYS M; KOT WK; PEGG IL

    2011-12-29

    system was reconfigured to enable testing of the baseline HLW or LAW off-gas trains to perform off-gas emissions testing with both LAW and HLW simulants in the present work. During 2002 and 2003, many of these off-gas components were tested individually and in an integrated manner with the DM1200 Pilot Melter. Data from these tests are being used to support engineering design confirmation and to provide data to support air permitting activities. In fiscal year 2004, the WTP Project was directed by the Office of River Protection (ORP) to comply with Environmental Protection Agency (EPA) Maximum Achievable Control Technology (MACT) requirements for organics. This requires that the combined melter and off-gas system have destruction and removal efficiency (DRE) of >99.99% for principal organic dangerous constituents (PODCs). In order to provide confidence that the melter and off-gas system are able to achieve the required DRE, testing has been directed with both LAW and HLW feeds. The tests included both 'normal' and 'challenge' WTP melter conditions in order to obtain data for the potential range of operating conditions for the WTP melters and off-gas components. The WTP Project, Washington State Department of Ecology, and ORP have agreed that naphthalene will be used for testing to represent semi-volatile organics and allyl alcohol will be used to represent volatile organics. Testing was also performed to determine emissions of halides, metals, products of incomplete combustion (PICs), dioxins, furans, coplanar PCBs, total hydrocarbons, and COX and NOX, as well as the particle size distribution (PSD) of particulate matter discharged at the end of the off-gas train. A description of the melter test requirements and analytical methods used is provided in the Test Plan for this work. Test Exceptions were subsequently issued which changed the TCO catalyst, added total organic emissions (TOE) to exhaust sampling schedule, and allowing modification of the

  17. Modeling of arylamide helix mimetics in the p53 peptide binding site of hDM2 suggests parallel and anti-parallel conformations are both stable.

    Directory of Open Access Journals (Sweden)

    Jonathan C Fuller

    Full Text Available The design of novel α-helix mimetic inhibitors of protein-protein interactions is of interest to pharmaceuticals and chemical genetics researchers as these inhibitors provide a chemical scaffold presenting side chains in the same geometry as an α-helix. This conformational arrangement allows the design of high affinity inhibitors mimicking known peptide sequences binding specific protein substrates. We show that GAFF and AutoDock potentials do not properly capture the conformational preferences of α-helix mimetics based on arylamide oligomers and identify alternate parameters matching solution NMR data and suitable for molecular dynamics simulation of arylamide compounds. Results from both docking and molecular dynamics simulations are consistent with the arylamides binding in the p53 peptide binding pocket. Simulations of arylamides in the p53 binding pocket of hDM2 are consistent with binding, exhibiting similar structural dynamics in the pocket as simulations of known hDM2 binders Nutlin-2 and a benzodiazepinedione compound. Arylamide conformations converge towards the same region of the binding pocket on the 20 ns time scale, and most, though not all dihedrals in the binding pocket are well sampled on this timescale. We show that there are two putative classes of binding modes for arylamide compounds supported equally by the modeling evidence. In the first, the arylamide compound lies parallel to the observed p53 helix. In the second class, not previously identified or proposed, the arylamide compound lies anti-parallel to the p53 helix.

  18. Polymorphisms of HLA-DM on Treatment Response to Interferon/Ribavirin in Patients with Chronic Hepatitis C Virus Type 1 Infection

    Science.gov (United States)

    Chen, Hongbo; Yao, Yinan; Wang, Yifan; Zhou, Hua; Xu, Tianxiang; Liu, Jing; Wang, Guocheng; Zhang, Yongfeng; Chen, Xiang; Liu, Qingwei; Huang, Peng; Yu, Rongbin

    2016-01-01

    Background: HLA-DM gene, which is related to antigen processing and presentation and located in the non-classical class-II region of human leukocyte antigen (HLA) region, may play a crucial role in chronic hepatitis C virus (HCV) infection treatment outcomes. The study was conducted to evaluate the role of the variant of several single nucleotide polymorphisms (SNPs) in HLA-DM gene in HCV treatment outcomes. Methods: We genotyped four SNPs from the candidate genes (HLA-DMA and DMB) in 336 patients who were treated with pegylated interferon-alpha and ribavirin (PEG IFN-α/RBV). Multivariate analysis of factors predicting sustained virological response (SVR) was conducted. Results: HLA-DMA rs1063478 and DMB rs23544 were independent factors of HCV treatment outcomes in Chinese Han population. Individuals who carried favorable genotypes of rs1063478TT and rs23544GG were more likely to achieve SVR {Dominant model: odds ratio (OR) = 2.05, 95% confidence interval (CI) = 1.24–3.41; OR = 2.04, 95% CI =1.23–3.35, respectively}. Rs23544, rs1063478, baseline glucose, baseline platelet and T4 level were independent predictors of SVR. The area under the receiver operating characteristic (ROC) curve (AUC) was 0.740. Conclusions: The genetic variation of rs1063478 and rs23544 are associated with the treatment outcomes in the Chinese Han population. PMID:27775635

  19. Effect of cationic molecules on the oxygen reduction reaction on fuel cell grade Pt/C (20 wt%) catalyst in potassium hydroxide (aq, 1 mol dm(-3)).

    Science.gov (United States)

    Ong, Ai Lien; Inglis, Kenneth K; Whelligan, Daniel K; Murphy, Sam; Varcoe, John R

    2015-05-14

    This study investigates the effect of 1 mmol dm(-3) concentrations of a selection of small cationic molecules on the performance of a fuel cell grade oxygen reduction reaction (ORR) catalyst (Johnson Matthey HiSPEC 3000, 20 mass% Pt/C) in aqueous KOH (1 mol dm(-3)). The cationic molecules studied include quaternary ammonium (including those based on bicyclic systems) and imidazolium types as well as a phosphonium example: these serve as fully solubilised models for the commonly encountered head-groups in alkaline anion-exchange membranes (AAEM) and anion-exchange ionomers (AEI) that are being developed for application in alkaline polymer electrolyte fuel cells (APEFCs), batteries and electrolysers. Both cyclic and hydrodynamic linear sweep rotating disk electrode voltammetry techniques were used. The resulting voltammograms and subsequently derived data (e.g. apparent electrochemical active surface areas, Tafel plots, and number of [reduction] electrons transferred per O2) were compared. The results show that the imidazolium examples produced the highest level of interference towards the ORR on the Pt/C catalyst under the experimental conditions used.

  20. DM rate at NLO and the impact of SUSY-QCD-corrections to (co-)annihilation-processes on neutralino dark matter

    Energy Technology Data Exchange (ETDEWEB)

    Harz, Julia [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Herrmann, Bjoern [Laboratoire d' Annecy de Physique Theorique, Annecy-le-Vieux (France); Klasen, Michael; Meinecke, Moritz; Steppeler, Patrick [Institute of Theoretical Physics Muenster (Germany); Kovarik, Karol [Karlsruher Institut fuer Technologie (KIT), Karlsruhe (Germany); Le Boulc' h, Quentin [Laboratoire de Physique Subatomique et de Cosmologie, Grenoble (France)

    2013-07-01

    A powerful method to constrain the parameter space of theories beyond the Standard Model is to compare the predicted dark matter relic density with cosmological precision measurements, in particular with WMAP- and the upcoming Planck-data. On the particle physics side, the main uncertainty on the relic density arises from the (co-)annihilation cross sections of the dark matter particle. After a motivation for including higher order corrections in the prediction of the relic density, the DM rate at NLO-project will be presented, a software package that allows for the computation of the neutralino (co-)annihilation cross sections including SUSY-QCD corrections at the one-loop level and the evaluation of their effect on the relic density using a link to the public codes MicrOMEGAs and DarkSUSY. Recent results of the impact of SUSY-QCD corrections on the neutralino (co-)annihilation cross section as well as further ongoing projects in the context of the DM rate at NLO-project are discussed.

  1. Measurement of Muon Annual Modulation and Muon-Induced Phosphorescence in NaI(Tl) Crystals with DM-Ice17

    CERN Document Server

    Cherwinka, J; Halzen, F; Heeger, K M; Hsu, L; Hubbard, A J F; Karle, A; Kauer, M; Kudryavtsev, V A; Lim, K E; Macdonald, C; Maruyama, R H; Paling, S; Pettus, W; Pierpoint, Z P; Reilly, B N; Robinson, M; Sandstrom, P; Spooner, N J C; Telfer, S; Yang, L

    2015-01-01

    We report the measurement of muons and muon-induced phosphorescence in DM-Ice17, a NaI(Tl) direct detection dark matter experiment at the South Pole. Muons are identified by the observed pulse shape and large energy deposition of their interaction in the crystals. The measured muon rate in DM-Ice17 is 2.93 $\\pm$ 0.04 $\\mu$/crystal/day with a modulation amplitude of 12.3 $\\pm$ 1.7%, consistent with expectation. Following muon interactions, we observe long-lived phosphorescence in the NaI(Tl) crystals with a decay time of 5.5 $\\pm$ 0.5 s. The prompt energy deposited by a muon is correlated to the amount of delayed phosphorescence, the brightest of which consist of tens of millions of photons. As they are distributed over tens of seconds, the rate and timing structure of photon arrivals do not mimic a scintillation signal above 2 keV$_\\mathrm{ee}$. While the properties of phosphorescence vary between individual crystals, the annually-modulating signal observed by DAMA cannot be accounted for by phosphorescence w...

  2. Polymorphisms of HLA-DM on Treatment Response to Interferon/Ribavirin in Patients with Chronic Hepatitis C Virus Type 1 Infection

    Directory of Open Access Journals (Sweden)

    Hongbo Chen

    2016-10-01

    Full Text Available Background: HLA-DM gene, which is related to antigen processing and presentation and located in the non-classical class-II region of human leukocyte antigen (HLA region, may play a crucial role in chronic hepatitis C virus (HCV infection treatment outcomes. The study was conducted to evaluate the role of the variant of several single nucleotide polymorphisms (SNPs in HLA-DM gene in HCV treatment outcomes. Methods: We genotyped four SNPs from the candidate genes (HLA-DMA and DMB in 336 patients who were treated with pegylated interferon-alpha and ribavirin (PEG IFN-α/RBV. Multivariate analysis of factors predicting sustained virological response (SVR was conducted. Results: HLA-DMA rs1063478 and DMB rs23544 were independent factors of HCV treatment outcomes in Chinese Han population. Individuals who carried favorable genotypes of rs1063478TT and rs23544GG were more likely to achieve SVR {Dominant model: odds ratio (OR = 2.05, 95% confidence interval (CI = 1.24–3.41; OR = 2.04, 95% CI =1.23–3.35, respectively}. Rs23544, rs1063478, baseline glucose, baseline platelet and T4 level were independent predictors of SVR. The area under the receiver operating characteristic (ROC curve (AUC was 0.740. Conclusions: The genetic variation of rs1063478 and rs23544 are associated with the treatment outcomes in the Chinese Han population.

  3. WJD 5th Anniversary Special Issues(2): Type 2 diabetes Structured SMBG in early management of T2DM: Contributions from the St Carlos study

    Institute of Scientific and Technical Information of China (English)

    Teresa; Ruiz; Gracia; Nuria; García; de; la; Torre; Lobo; Alejandra; Durán; Rodríguez; Hervada; Alfonso; L; Calle; Pascual

    2014-01-01

    Diabetes mellitus type 2(T2DM)is a global pandemic that will affect 300 million people in the next decade.It has been shown that early and aggressive treatment of T2DM from the onset decreases complications,and the patient’s active role is necessary to achieve better glycemic control.In order to achieve glycemic control targets,an active attitude in patients is needed,and selfmonitoring of blood glucose(SMBG)plays a significant role.Nowadays,SMBG has become an important component of modern therapy for diabetes mellitus,and is even more useful if it is performed in a structured way.SMBG aids physicians and patients to achieve a specific level of glycemic control and to prevent hypoglycemia.In addition,SMBG empowers patients to achieve nutritional and physical activity goals,and helps physicians to optimize the different hypoglycemic therapies as demonstrated in the St Carlos study.This article describes the different ways of using this educational and therapeutic tool from the medical point of view as well as from the patient’s perspective.

  4. Communication between TMS320DM642 and PC/104+ in Traffic Video Supervisory System%交通视频监控系统中TMS320DM642与嵌入式PC/104+模块的通信

    Institute of Scientific and Technical Information of China (English)

    谢雅端; 刘富强; Matthieu Sauce

    2005-01-01

    描述了TI公司媒体处理器TMS320DM642 HPI的主要特点,介绍了PCI桥接器PCI2040的结构原理,提出了通过PCI2040实现TMS320DM642与PC/104+模块进行通信的设计方案.本方案充分利用PCI总线的高数据吞吐能力和DSP的HPI接口功能,可在DSP和PC/104+模块之间实现较高的数据传输率,实现了PC/104+实时读/写DSP任意片内存储单元的内容.最后给出交通视频监控系统的设计方案,详细说明了通信过程的软件编程.

  5. Intermittent Vagal Nerve Block for Improvements in Obesity, Cardiovascular Risk Factors, and Glycemic Control in Patients with Type 2 Diabetes Mellitus: 2-Year Results of the VBLOC DM2 Study.

    Science.gov (United States)

    Shikora, Scott A; Toouli, James; Herrera, Miguel F; Kulseng, Bård; Brancatisano, Roy; Kow, Lilian; Pantoja, Juan P; Johnsen, Gjermund; Brancatisano, Anthony; Tweden, Katherine S; Knudson, Mark B; Billington, Charles J; Billingto, Charles J

    2016-05-01

    One-year results of the VBLOC DM2 study found that intermittent vagal blocking (VBLOC therapy) was safe among subjects with obesity and type 2 diabetes mellitus (T2DM) and led to significant weight loss and improvements in glycemic parameters and cardiovascular risk factors. Longer-term data are needed to determine whether the results are sustained. VBLOC DM2 is a prospective, observational study of 28 subjects with T2DM and body mass index (BMI) between 30 and 40 kg/m(2) to assess mid-term safety and weight loss and improvements in glycemic parameters, and other cardiovascular risk factors with VBLOC therapy. Continuous outcome variables are reported using mixed models. At 24 months, the mean percentage of excess weight loss was 22% (95% CI, 15 to 28, p obesity and glycemic control were largely sustained after 2 years of treatment with VBLOC therapy with a well-tolerated risk profile.

  6. A retrospective analysis of a societal experiment among the Danish population suggests that exposure to extra doses of vitamin A during fetal development may lower type 2 diabetes mellitus (T2DM) risk later in life

    DEFF Research Database (Denmark)

    Keller, Amélie; Ängquist, Lars; Jacobsen, Ramune

    2017-01-01

    had been exposed to the extra vitamin A from fortification during pregnancy had a lower risk of developing T2DM in adult life, compared with offspring of mothers exposed to less vitamin A. Individuals from birth cohorts with the higher prenatal vitamin A exposure (born 1 December 1962-31 March 1964......Vitamin A deficiency has been associated with impaired fetal pancreatic development and increased risk of developing type 2 diabetes mellitus (T2DM). In 1962, mandatory margarine fortification with vitamin A was increased by 25 % in Denmark. We aimed to determine whether offspring of mothers who......DM by vitamin A exposure level. A total of 193 803 individuals were followed up until midlife. Our results showed that individuals exposed prenatally to extra vitamin A from fortified margarine had a lower risk of developing T2DM than those exposed to lower levels: OR 0·88; 95 % CI 0·81, 0·95, P=0...

  7. Incorporation of Rubber Powder as Filler in a New Dry-Hybrid Technology: Rheological and 3D DEM Mastic Performances Evaluation

    Science.gov (United States)

    Vignali, Valeria; Mazzotta, Francesco; Sangiorgi, Cesare; Simone, Andrea; Lantieri, Claudio; Dondi, Giulio

    2016-01-01

    In recent years, the use of crumb rubber as modifier or additive within asphalt concretes has allowed obtaining mixtures able to bind high performances to recovery and reuse of discarded tires. To date, the common technologies that permit the reuse of rubber powder are the wet and dry ones. In this paper, a dry-hybrid technology for the production of Stone Mastic Asphalt mixtures is proposed. It allows the use of the rubber powder as filler, replacing part of the limestone one. Fillers are added and mixed with a high workability bitumen, modified with SBS (styrene-butadiene-styrene) polymer and paraffinic wax. The role of rubber powder and limestone filler within the bituminous mastic has been investigated through two different approaches. The first one is a rheological approach, which comprises a macro-scale laboratory analysis and a micro-scale DEM simulation. The second, instead, is a performance approach at high temperatures, which includes Multiple Stress Creep Recovery tests. The obtained results show that the rubber works as filler and it improves rheological characteristics of the polymer modified bitumen. In particular, it increases stiffness and elasticity at high temperatures and it reduces complex modulus at low temperatures. PMID:28773965

  8. Incorporation of Rubber Powder as Filler in a New Dry-Hybrid Technology: Rheological and 3D DEM Mastic Performances Evaluation

    Directory of Open Access Journals (Sweden)

    Valeria Vignali

    2016-10-01

    Full Text Available In recent years, the use of crumb rubber as modifier or additive within asphalt concretes has allowed obtaining mixtures able to bind high performances to recovery and reuse of discarded tires. To date, the common technologies that permit the reuse of rubber powder are the wet and dry ones. In this paper, a dry-hybrid technology for the production of Stone Mastic Asphalt mixtures is proposed. It allows the use of the rubber powder as filler, replacing part of the limestone one. Fillers are added and mixed with a high workability bitumen, modified with SBS (styrene-butadiene-styrene polymer and paraffinic wax. The role of rubber powder and limestone filler within the bituminous mastic has been investigated through two different approaches. The first one is a rheological approach, which comprises a macro-scale laboratory analysis and a micro-scale DEM simulation. The second, instead, is a performance approach at high temperatures, which includes Multiple Stress Creep Recovery tests. The obtained results show that the rubber works as filler and it improves rheological characteristics of the polymer modified bitumen. In particular, it increases stiffness and elasticity at high temperatures and it reduces complex modulus at low temperatures.

  9. Mobile health technology in the prevention and management of Type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Shruti Muralidharan

    2017-01-01

    Full Text Available Essential steps in diabetes prevention and management include translating research into the real world, improving access to health care, empowering the community, collaborative efforts involving physicians, diabetes educators, nurses, and public health scientists, and access to diabetes prevention and management efforts. Mobile phone technology has shown wide acceptance across various ages and socioeconomic groups and offers several opportunities in health care including self-management as well as prevention of Type 2 diabetes mellitus (T2DM. The future seems to lie in mobile health (mHealth applications that can use embedded technology to showcase advanced uses of a smartphone to help with prevention and management of chronic disorders such as T2DM. This article presents a narrative review of the mHealth technologies used for the prevention and management of T2DM. Majority (48% of the studies used short message service (SMS technology as their intervention while some studies (29% incorporated applications for medication reminders and insulin optimization for T2DM management. Few studies (23% showed that, along with mHealth technology, health-care professionals' support resulted in added positive outcomes for the patients. This review highlights the fact that an mHealth intervention need not be restricted to SMS alone.

  10. hs-CRP与2型糖尿病伴有高血压的关系%High plasmid hs-CRP level in patients with DM2 and HBP Daqing Oilfield General Hospital Laboratory Medicine

    Institute of Scientific and Technical Information of China (English)

    张丽娜; 车璐; 曹艳菲; 李新娜; 崔颖; 寇筱囡; 陈刚

    2013-01-01

    Objective We study if plasma levels of hs-CRP related to DM2+HBP patients. Methods hs-CRP were measured in subjects with DM2 and /or HBP, including 13 patients with DM2, 17 patients with HBP, 34 patients with DM2+HBP, and 25 healthy test control subjects. hs-CRP tested by Electro-Chemiluminescence Immuno Assay (ECLI). Results The plasma hs-CRP levels were significantly lower in the controls than in the DM2+HBP group (p<0.05), DM2 associated with HBP was also correlated with increased plasma hs-CRP levels (n=89, r =0.25, p=0.0160). Conclusions This study suggests that patients with two associated diseases have a more active inflammatory state.%目的:研究炎性反应标志物超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)与2型糖尿病(type 2 diabetes mel itus, DM2)伴随或者不伴随高血压是否存在相关性。方法入组13例2型糖尿病患者无合并症,17例高血压患者(high blood pressure, HBP),34例2型糖尿病伴随高血压患者(DM2+HBP),25例健康体检对照组,电化学免疫发光法测定血清hs-CRP。结果健康对照组hs-CRP水平明显低于HBP+DM2患者(p<0.05),DM2伴随HBP与hs-CRP水平显著相关(n=89, r=0.25, p=0.0160)。讨论 DM2+HBP患者具有相对活跃的炎性状态。

  11. Application of a PK-PD Modeling and Simulation-Based Strategy for Clinical Translation of Antibody-Drug Conjugates: a Case Study with Trastuzumab Emtansine (T-DM1).

    Science.gov (United States)

    Singh, Aman P; Shah, Dhaval K

    2017-04-03

    Successful clinical translation of antibody-drug conjugates (ADCs) can be challenging due to complex pharmacokinetics and differences between preclinical and clinical tumors. To facilitate this translation, we have developed a general pharmacokinetic-pharmacodynamic (PK-PD) modeling and simulation (M&S)-based strategy for ADCs. Here we present the validation of this strategy using T-DM1 as a case study. A previously developed preclinical tumor disposition model for T-DM1 (Singh and Shah, AAPSJ. 2015; 18(4):861-875) was used to develop a PK-PD model that can characterize in vivo efficacy of T-DM1 in preclinical tumor models. The preclinical data was used to estimate the efficacy parameters for T-DM1. Human PK of T-DM1 was a priori predicted using allometric scaling of monkey PK parameters. The predicted human PK, preclinically estimated efficacy parameters, and clinically observed volume and growth parameters for breast cancer were combined to develop a translated clinical PK-PD model for T-DM1. Clinical trial simulations were performed using the translated PK-PD model to predict progression-free survival (PFS) and objective response rates (ORRs) for T-DM1. The model simulated PFS rates for HER2 1+ and 3+ populations were comparable to the rates observed in three different clinical trials. The model predicted only a modest improvement in ORR with an increase in clinically approved dose of T-DM1. However, the model suggested that a fractionated dosing regimen (e.g., front loading) may provide an improvement in the efficacy. In general, the PK-PD M&S-based strategy presented here is capable of a priori predicting the clinical efficacy of ADCs, and this strategy has been now retrospectively validated for all clinically approved ADCs.

  12. CHP Technologies

    Science.gov (United States)

    Learn about CHP technologies, including reciprocating engines, combustion turbines, steam turbines, microturbines, fuel cells, and waste heat to power. Access the Catalog of CHP Technologies and the Biomass CHP Catalog of Technologies.

  13. Assistive Technology

    Science.gov (United States)

    ... Page Resize Text Printer Friendly Online Chat Assistive Technology Assistive technology (AT) is any service or tool that helps ... be difficult or impossible. For older adults, such technology may be a walker to improve mobility or ...

  14. First Results of the GPS.DM Observatory: Search for Dark Matter and Exotic Physics with Atomic Clocks and GPS Constellation

    Science.gov (United States)

    Roberts, Benjamin; Blewitt, Geoff; Dailey, Conner; Pospelov, Maxim; Rollings, Alex; Sherman, Jeff; Williams, Wyatt; Derevianko, Andrei; GPS. DM Collaboration

    2017-01-01

    Despite the overwhelming cosmological evidence for the existence of dark matter, and the considerable effort of the scientific community over decades, there is no evidence for dark matter in terrestrial experiments. The GPS.DM observatory uses the existing GPS constellation as a 50,000 km-aperture sensor array, analysing the satellite and terrestrial atomic clock data for exotic physics signatures. In particular, the collaboration searches for evidence of transient variations of fundamental constants correlated with the Earth's galactic motion through the dark matter halo. There already exists more than 10 years of good clock timing data that can be used in the search. This type of search is particularly sensitive to exotic forms of dark matter, such as topological defects. Supported by the NSF.

  15. Solid supported microwave induced synthesis of imidazole–pyrimidine hybrids: Antimicrobial evaluation and docking study as 14DM-CPY51 inhibitors

    Directory of Open Access Journals (Sweden)

    Naziyanaz B. Pathan

    2016-09-01

    Full Text Available As part of our exploration for new antifungal agents, substituted 4,5-diphenyl imidazolyl pyrimidine hybrids were synthesized. A series of substituted ethyl 1,2,3,6-tetrahydro-4-methyl-2-oxo/thioxo-6-phenyl-1-(4,5-diphenyl-1-H-imidazol-2-yl pyrimidine-5-carboxylates have been studied for their binding active sites of cytochrome P450 14α-sterol demethylase CPY51 enzyme. For comparison, the binding behavior of known 14DM selective (Fluconazole and non-selective (Clotrimazole, Miconazole, Griesofulvin drugs has also been studied. Synthesized compounds were screened for their in vitro antibacterial activity against Staphylococcus aureus, Salmonella typhi, Pseudomonas aurogenosa and Klebsiella pneumonae and also antifungal activity against the opportunistic pathogens Candida albicans.

  16. Final Report - Glass Formulation Development and DM10 Melter Testing with ORP LAW Glasses, VSL-09R1510-2, Rev. 0, dated 6/12/09

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A.; Pegg, I. L.; Matlack, K. S.; Joseph, I.; Muller, I. S.; Gong, W.

    2013-11-13

    The principal objective of the work described in this Final Report is to extend the glass formulation methodology developed in the earlier work by development of acceptable glass compositions for four LAW compositions specified by ORP that cover the range of sulfate to sodium and potassium to sodium ratios expected in Hanford LAW. The glass formulations were designed to exclude titanium and iron as glass former additives, while tin and vanadium as glass former additives were evaluated for beneficial effects in increasing waste loading in the glasses. This was accomplished through a combination of crucible-scale tests and tests on the DM10 melter system. This melter is the most efficient melter platform for screening glass compositions over a wide range of sulfate concentrations and therefore was selected for the present tests. The current tests provide information on melter processing characteristics and off-gas data, including sulfur incorporation and partitioning.

  17. Characterization and Optimization of a Novel Protein–Protein Interaction Biosensor High-Content Screening Assay to Identify Disruptors of the Interactions Between p53 and hDM2

    Science.gov (United States)

    Dudgeon, Drew D.; Shinde, Sunita N.; Shun, Tong Ying; Lazo, John S.; Strock, Christopher J.; Giuliano, Kenneth A.; Taylor, D. Lansing; Johnston, Patricia A.

    2010-01-01

    Abstract We present here the characterization and optimization of a novel imaging-based positional biosensor high-content screening (HCS) assay to identify disruptors of p53-hDM2 protein–protein interactions (PPIs). The chimeric proteins of the biosensor incorporated the N-terminal PPI domains of p53 and hDM2, protein targeting sequences (nuclear localization and nuclear export sequence), and fluorescent reporters, which when expressed in cells could be used to monitor p53-hDM2 PPIs through changes in the subcellular localization of the hDM2 component of the biosensor. Coinfection with the recombinant adenovirus biosensors was used to express the NH-terminal domains of p53 and hDM2, fused to green fluorescent protein and red fluorescent protein, respectively, in U-2 OS cells. We validated the p53-hDM2 PPI biosensor (PPIB) HCS assay with Nutlin-3, a compound that occupies the hydrophobic pocket on the surface of the N-terminus of hDM2 and blocks the binding interactions with the N-terminus of p53. Nutlin-3 disrupted the p53-hDM2 PPIB in a concentration-dependent manner and provided a robust, reproducible, and stable assay signal window that was compatible with HCS. The p53-hDM2 PPIB assay was readily implemented in HCS and we identified four (4) compounds in the 1,280-compound Library of Pharmacologically Active Compounds that activated the p53 signaling pathway and elicited biosensor signals that were clearly distinct from the responses of inactive compounds. Anthracycline (topoisomerase II inhibitors such as mitoxantrone and ellipticine) and camptothecin (topoisomerase I inhibitor) derivatives including topotecan induce DNA double strand breaks, which activate the p53 pathway through the ataxia telangiectasia mutated-checkpoint kinase 2 (ATM-CHK2) DNA damage response pathway. Although mitoxantrone, ellipticine, camptothecin, and topotecan all exhibited concentration-dependent disruption of the p53-hDM2 PPIB, they were much less potent than Nutlin-3. Further

  18. Characterization and optimization of a novel protein-protein interaction biosensor high-content screening assay to identify disruptors of the interactions between p53 and hDM2.

    Science.gov (United States)

    Dudgeon, Drew D; Shinde, Sunita N; Shun, Tong Ying; Lazo, John S; Strock, Christopher J; Giuliano, Kenneth A; Taylor, D Lansing; Johnston, Patricia A; Johnston, Paul A

    2010-08-01

    We present here the characterization and optimization of a novel imaging-based positional biosensor high-content screening (HCS) assay to identify disruptors of p53-hDM2 protein-protein interactions (PPIs). The chimeric proteins of the biosensor incorporated the N-terminal PPI domains of p53 and hDM2, protein targeting sequences (nuclear localization and nuclear export sequence), and fluorescent reporters, which when expressed in cells could be used to monitor p53-hDM2 PPIs through changes in the subcellular localization of the hDM2 component of the biosensor. Coinfection with the recombinant adenovirus biosensors was used to express the NH-terminal domains of p53 and hDM2, fused to green fluorescent protein and red fluorescent protein, respectively, in U-2 OS cells. We validated the p53-hDM2 PPI biosensor (PPIB) HCS assay with Nutlin-3, a compound that occupies the hydrophobic pocket on the surface of the N-terminus of hDM2 and blocks the binding interactions with the N-terminus of p53. Nutlin-3 disrupted the p53-hDM2 PPIB in a concentration-dependent manner and provided a robust, reproducible, and stable assay signal window that was compatible with HCS. The p53-hDM2 PPIB assay was readily implemented in HCS and we identified four (4) compounds in the 1,280-compound Library of Pharmacologically Active Compounds that activated the p53 signaling pathway and elicited biosensor signals that were clearly distinct from the responses of inactive compounds. Anthracycline (topoisomerase II inhibitors such as mitoxantrone and ellipticine) and camptothecin (topoisomerase I inhibitor) derivatives including topotecan induce DNA double strand breaks, which activate the p53 pathway through the ataxia telangiectasia mutated-checkpoint kinase 2 (ATM-CHK2) DNA damage response pathway. Although mitoxantrone, ellipticine, camptothecin, and topotecan all exhibited concentration-dependent disruption of the p53-hDM2 PPIB, they were much less potent than Nutlin-3. Further, their

  19. Thrombin Receptor-Activating Protein (TRAP-Activated Akt Is Involved in the Release of Phosphorylated-HSP27 (HSPB1 from Platelets in DM Patients

    Directory of Open Access Journals (Sweden)

    Haruhiko Tokuda

    2016-05-01

    Full Text Available It is generally known that heat shock protein 27 (HSP27 is phosphorylated through p38 mitogen-activated protein (MAP kinase. We have previously reported that HSP27 is released from human platelets associated with collagen-induced phosphorylation. In the present study, we conducted an investigation into the effect of thrombin receptor-activating protein (TRAP on the release of HSP27 in platelets in type 2 diabetes mellitus (DM patients. The phosphorylated-HSP27 levels induced by TRAP were directly proportional to the aggregation of platelets. The levels of phosphorylated-HSP27 (Ser-78 were correlated with the levels of phosphorylated-p38 MAP kinase and phosphorylated-Akt in the platelets stimulated by 10 µM TRAP but not with those of phosphorylated-p44/p42 MAP kinase. The levels of HSP27 released from the TRAP (10 µM-stimulated platelets were correlated with the levels of phosphorylated-HSP27 in the platelets. The released platelet-derived growth factor-AB (PDGF-AB levels were in parallel with the HSP27 levels released from the platelets stimulated by 10 µM TRAP. Although the area under the curve (AUC of small aggregates (9–25 µm induced by 10 µM TRAP showed no significant correlation with the released HSP27 levels, AUC of medium aggregates (25–50 µm, large aggregates (50–70 µm and light transmittance were significantly correlated with the released HSP27 levels. TRAP-induced phosphorylation of HSP27 was truly suppressed by deguelin, an inhibitor of Akt, in the platelets from a healthy subject. These results strongly suggest that TRAP-induced activation of Akt in addition to p38 MAP kinase positively regulates the release of phosphorylated-HSP27 from human platelets, which is closely related to the platelet hyper-aggregation in type 2 DM patients.

  20. Lactuca saligna, a non-host for lettuce downy mildew ( Bremia lactucae), harbors a new race-specific Dm gene and three QTLs for resistance.

    Science.gov (United States)

    Jeuken, M.; Lindhout, P.

    2002-08-01

    Lactuca sativa (lettuce) is susceptible to Bremia lactucae (downy mildew). In cultivated and wild Lactuca species, Dm genes have been identified that confer race-specific resistance. However, these genes were soon rendered ineffective by adaptation of the pathogen. Lactuca saligna (wild lettuce) is resistant to all downy mildew races and can be considered as a non-host. Therefore, L. saligna might be an alternative source for a more-durable resistance to downy mildew in lettuce. In order to analyze this resistance, we have developed an F(2) population based on a resistant L. saligna x susceptible L. sativa cross. This F(2) population was fingerprinted with AFLP markers and tested for resistance to two Bremia races NL14 and NL16. The F(2) population showed a wide and continuous range of resistance levels from completely resistant to completely susceptible. By comparison of disease tests, we observed a quantitative resistance against both Bremia races as well as a race-specific resistance to Bremia race NL16 and not to NL14. QTL mapping revealed a qualitative gene ( R39) involved in the race-specific resistance and three QTLs ( RBQ1, RBQ2 and RBQ3) involved in the quantitative resistance. The qualitative gene R39 is a dominant gene that gives nearly complete resistance to race NL16 in L. saligna CGN 5271 and therefore it showed features similar to Dm genes. The three QTLs explained 51% of the quantitative resistance against NL14, which indicated that probably only the major QTLs have been detected in this F(2) population. The perspectives for breeding for durable resistance are discussed.

  1. Bile-acid-mediated decrease in endoplasmic reticulum stress: a potential contributor to the metabolic benefits of ileal interposition surgery in UCD-T2DM rats

    Directory of Open Access Journals (Sweden)

    Bethany P. Cummings

    2013-03-01

    Post-operative increases in circulating bile acids have been suggested to contribute to the metabolic benefits of bariatric surgery; however, their mechanistic contributions remain undefined. We have previously reported that ileal interposition (IT surgery delays the onset of type 2 diabetes in UCD-T2DM rats and increases circulating bile acids, independently of effects on energy intake or body weight. Therefore, we investigated potential mechanisms by which post-operative increases in circulating bile acids improve glucose homeostasis after IT surgery. IT, sham or no surgery was performed on 2-month-old weight-matched male UCD-T2DM rats. Animals underwent an oral fat tolerance test (OFTT and serial oral glucose tolerance tests (OGTT. Tissues were collected at 1.5 and 4.5 months after surgery. Cell culture models were used to investigate interactions between bile acids and ER stress. IT-operated animals exhibited marked improvements in glucose and lipid metabolism, with concurrent increases in postprandial glucagon-like peptide-1 (GLP-1 secretion during the OFTT and OGTTs, independently of food intake and body weight. Measurement of circulating bile acid profiles revealed increases in circulating total bile acids in IT-operated animals, with a preferential increase in circulating cholic acid concentrations. Gut microbial populations were assessed as potential contributors to the increases in circulating bile acid concentrations, which revealed proportional increases in Gammaproteobacteria in IT-operated animals. Furthermore, IT surgery decreased all three sub-arms of ER stress signaling in liver, adipose and pancreas tissues. Amelioration of ER stress coincided with improved insulin signaling and preservation of β-cell mass in IT-operated animals. Incubation of hepatocyte, adipocyte and β-cell lines with cholic acid decreased ER stress. These results suggest that postoperative increases in circulating cholic acid concentration contribute to improvements in

  2. Clinical signature and pathogenetic factors of diabetes associated with pancreas disease (T3cDM): a prospective observational study in surgical patients.

    Science.gov (United States)

    Balzano, Gianpaolo; Dugnani, Erica; Pasquale, Valentina; Capretti, Giovanni; Radaelli, Maria Grazia; Garito, Tania; Stratta, Gregorio; Nini, Alessandro; Di Fenza, Raffaele; Castoldi, Renato; Staudacher, Carlo; Reni, Michele; Scavini, Marina; Doglioni, Claudio; Piemonti, Lorenzo

    2014-10-01

    To characterize the clinical signature and etiopathogenetic factors of diabetes associated with pancreas disease [type 3 diabetes mellitus (T3cDM)]. To estimate incidence and identify predictors of both diabetes onset and remission after pancreatic surgery. A prospective observational study was conducted. From January 2008 to December 2012, patients (n = 651) with new diagnosis of pancreatic disease admitted to the Pancreatic Surgery Unit of the San Raffaele Scientific Institute were evaluated. Hospital and/or outpatient medical records were reviewed. Blood biochemical values including fasting blood glucose, insulin and/or C-peptide, glycosylated hemoglobin and anti-islet antibodies were determined. Diabetes onset was assessed after surgery and during follow-up. At baseline, the prevalence of diabetes was 38 % (age of onset 64 ± 11 years). In most cases, diabetes occurred within 48 months from pancreatic disease diagnosis. Among different pancreatic diseases, minor differences were observed in diabetes characteristics, with the exception of the prevalence. Diabetes appeared associated with classical risk factors for type 2 diabetes (i.e., age, sex, family history of diabetes and body mass index), and both beta-cell dysfunction and insulin resistance appeared relevant determinants. The prevalence of adult-onset autoimmune diabetes was as previously reported within type 2 diabetes. Within a few days after surgery, either diabetes remission or new-onset diabetes was observed. In patients with pancreatic cancer, no difference in diabetes remission was observed after palliative or resective surgery. Classical risk factors for type 2 diabetes were associated with the onset of diabetes after surgery. T3cDM appeared as a heterogeneous entity strongly overlapped with type 2 diabetes.

  3. Cirurgia bariátrica e metabólica e complicações microvasculares do diabetes mellitus tipo 2 (DM2

    Directory of Open Access Journals (Sweden)

    Ricardo Cohen

    2015-09-01

    Full Text Available ResumoIntrodução:O diabetes mellitus tipo 2 (DM2 é caracterizado por uma desregulação metabólica, originando complicações microvasculares, mais especificamente a retinopatia, nefropatia e a neuropatia. A prevenção e tratamento das complicações são alvo da farmacoterapia, porém, evidências demonstram que a cirurgia bariátrica/metabólica é superior ao melhor tratamento farmacológico, pois apresenta melhor controle da glicemia, hipertensão e dislipidemias.Métodos:Por meio de pesquisa no PubMed, são discutidas as recentes publicações que evidenciam o efeito positivo das intervenções cirúrgicas sobre as complicações microvasculares, como melhora da microalbuminúria e mesmo preservação de função renal.Discussão:Existem evidências de benefício das operações bariátricas/metabólicas sobre a nefropatia diabética. Os dados sobre retinopatia são ainda ambivalentes. Na literatura, há uma diferença significativa no benefício da cirurgia em neuropatia.Conclusão:Apesar de resultados surpreendentes e positivos, para que se estabeleça definitivamente o papel da cirurgia bariátrica/metabólica nas complicações micovasculares do DM2, há a necessidade de novos estudos randomizados controlados e prospectivos.

  4. Relative factors to sarcopenia in T2DM patients%2型糖尿病患者肌少症的相关因素

    Institute of Scientific and Technical Information of China (English)

    吴佳佳; 王炜; 祝捷; 吴玉洁; 陈超; 邢学农

    2016-01-01

    , age, 25-hydroxyvitamin D (25OHD) level and diabetic duration in type 2 diabetes mellitus (T2DM) patients.Methods Sixty controls, one hun-dred thirty-two patients with T2DM were enrolled in the study .All the subjects underwent body composition measurement by dual-energy X-ray absorptiometry (DXA), and the serum 25OHD level were measured by electrochemiluminescence method.Sarcopenia was diagnosed by the Baumgartner diagnostic criteria .The prevalence of sarcopenia in T2DM group and the control group was compared , and in different gender respectively .The subjects in the two groups were divided in-to five subgroups according to age .Afterwards, these subgroups were divided into vitamin D severe deficiency ( <10 ng/mL), deficiency (≥10 and <20 ng/mL), insufficiency (≥20 and <30 ng/mL) and sufficiency group (≥30 ng/mL).T2DM group was divided into a-e groups according to the diabetic duration .The prevalence of sarcopenia in each group was compared .Results Compared with the control group (11.7%), the prevalence of sarcopenia in T2DM group (24.2%) was higher.The prevalence of sarcopenia in men was higher than in women ( T2DM group: 32.8%/15.4%; the control group: 23.1%/2.9%, P<0.05 ) .The prevalence of sarcopenia in subjects older than 80 years old (T2DM group: 50.0%, the control group: 42.9%) was the highest, and the subjects with sarcopenia was older than those without sarcopenia (P<0.05).The prevalence of sarcopenia in vitamin D severe deficiency group (T2DM group: 41.7%; the control group: 30.0%) was higher, and the 25OHD level of subjects with sarcopenia was lower than those without sarcopenia ( P<0.05 ) .The prevalence of sarcopenia in diabetic duration more than 20 years group (61.1%) was higher, and the diabetic duration of subjects with sarcopenia was longer than those without sarcopenia (P<0.05).Conclusion Aging, gender, T2DM and diabetic duration are risk factors for sarcopenia .Low serum 25OHD level may be related to sarcopenia .

  5. Construction of a multisite DataLink using electronic health records for the identification, surveillance, prevention, and management of diabetes mellitus: the SUPREME-DM project.

    Science.gov (United States)

    Nichols, Gregory A; Desai, Jay; Elston Lafata, Jennifer; Lawrence, Jean M; O'Connor, Patrick J; Pathak, Ram D; Raebel, Marsha A; Reid, Robert J; Selby, Joseph V; Silverman, Barbara G; Steiner, John F; Stewart, W F; Vupputuri, Suma; Waitzfelder, Beth

    2012-01-01

    Electronic health record (EHR) data enhance opportunities for conducting surveillance of diabetes. The objective of this study was to identify the number of people with diabetes from a diabetes DataLink developed as part of the SUPREME-DM (SUrveillance, PREvention, and ManagEment of Diabetes Mellitus) project, a consortium of 11 integrated health systems that use comprehensive EHR data for research. We identified all members of 11 health care systems who had any enrollment from January 2005 through December 2009. For these members, we searched inpatient and outpatient diagnosis codes, laboratory test results, and pharmaceutical dispensings from January 2000 through December 2009 to create indicator variables that could potentially identify a person with diabetes. Using this information, we estimated the number of people with diabetes and among them, the number of incident cases, defined as indication of diabetes after at least 2 years of continuous health system enrollment. The 11 health systems contributed 15,765,529 unique members, of whom 1,085,947 (6.9%) met 1 or more study criteria for diabetes. The nonstandardized proportion meeting study criteria for diabetes ranged from 4.2% to 12.4% across sites. Most members with diabetes (88%) met multiple criteria. Of the members with diabetes, 428,349 (39.4%) were incident cases. The SUPREME-DM DataLink is a unique resource that provides an opportunity to conduct comparative effectiveness research, epidemiologic surveillance including longitudinal analyses, and population-based care management studies of people with diabetes. It also provides a useful data source for pragmatic clinical trials of prevention or treatment interventions.

  6. FINAL REPORT REGULATORY OFF GAS EMISSIONS TESTING ON THE DM1200 MELTER SYSTEM USING HLW AND LAW SIMULANTS VSL-05R5830-1 REV 0 10/31/05

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; BRANDYS M; KOT WK; PEGG IL

    2011-12-29

    system was reconfigured to enable testing of the baseline HLW or LAW off-gas trains to perform off-gas emissions testing with both LAW and HLW simulants in the present work. During 2002 and 2003, many of these off-gas components were tested individually and in an integrated manner with the DM1200 Pilot Melter. Data from these tests are being used to support engineering design confirmation and to provide data to support air permitting activities. In fiscal year 2004, the WTP Project was directed by the Office of River Protection (ORP) to comply with Environmental Protection Agency (EPA) Maximum Achievable Control Technology (MACT) requirements for organics. This requires that the combined melter and off-gas system have destruction and removal efficiency (DRE) of >99.99% for principal organic dangerous constituents (PODCs). In order to provide confidence that the melter and off-gas system are able to achieve the required DRE, testing has been directed with both LAW and HLW feeds. The tests included both 'normal' and 'challenge' WTP melter conditions in order to obtain data for the potential range of operating conditions for the WTP melters and off-gas components. The WTP Project, Washington State Department of Ecology, and ORP have agreed that naphthalene will be used for testing to represent semi-volatile organics and allyl alcohol will be used to represent volatile organics. Testing was also performed to determine emissions of halides, metals, products of incomplete combustion (PICs), dioxins, furans, coplanar PCBs, total hydrocarbons, and COX and NOX, as well as the particle size distribution (PSD) of particulate matter discharged at the end of the off-gas train. A description of the melter test requirements and analytical methods used is provided in the Test Plan for this work. Test Exceptions were subsequently issued which changed the TCO catalyst, added total organic emissions (TOE) to exhaust sampling schedule, and allowing modification of the

  7. Instrument for assessing mobile technology acceptability in diabetes self-management: a validation and reliability study.

    Science.gov (United States)

    Frandes, Mirela; Deiac, Anca V; Timar, Bogdan; Lungeanu, Diana

    2017-01-01

    Nowadays, mobile technologies are part of everyday life, but the lack of instruments to assess their acceptability for the management of chronic diseases makes their actual adoption for this purpose slow. The objective of this study was to develop a survey instrument for assessing patients' attitude toward and intention to use mobile technology for diabetes mellitus (DM) self-management, as well as to identify sociodemographic characteristics and quality of life factors that affect them. We first conducted the documentation and instrument design phases, which were subsequently followed by the pilot study and instrument validation. Afterward, the instrument was administered 103 patients (median age: 37 years; range: 18-65 years) diagnosed with type 1 or type 2 DM, who accepted to participate in the study. The reliability and construct validity were assessed by computing Cronbach's alpha and using factor analysis, respectively. The instrument included statements about the actual use of electronic devices for DM management, interaction between patient and physician, attitude toward using mobile technology, and quality of life evaluation. Cronbach's alpha was 0.9 for attitude toward using mobile technology and 0.97 for attitude toward using mobile device applications for DM self-management. Younger patients (Spearman's ρ=-0.429; Ptruthfulness and easiness to use.

  8. Clinical Significance of Changes on Serum Cortisol and ACTH in Impaired Glucose Regulation and Type 2 Diabetics%血清Cortisol及ACTH在糖调节受损和DM2患者中的变化及意义

    Institute of Scientific and Technical Information of China (English)

    周晓莉; 饶一武

    2013-01-01

    目的:探讨血清皮质醇(Cortisol)及促肾上腺皮质激素(ACTH)在糖调节受损(IGR)和2型糖尿病(DM2)患者中的变化及意义.方法:比较糖调节受损组、DM2组与正常对照组在8:00及24:00两次的血清Cortisol和ACTH水平;并进一步将糖调节受损组分为糖耐量减低(IGT)组和空腹血糖受损(IFG)组分别与DM2组比较8:00及24:00两次的血清Cortisol和ACTH水平.结果:①IGR和DM2组患者8:00及24:00两次的Cortisol和ACTH较正常对照组升高,差异有统计学意义(P<0.01或P< 0.05);②DM2组8:00血清Cortisol和ACTH水平低于IGT组和IFG组,差异有统计学意义(P<0.01或P< 0.05);③IGR和DM2患者血清Cortisol及ACTH水平相关分析显示Cortisol、ACTH间呈正相关(r=0.4002、0.4231,P<0.05).结论:IGR和DM2患者体内存在以血清Cortisol及ACTH分泌增高为主要表现的HPA轴功能紊乱,且随病情进展HPA轴有功能"耗竭"的趋势.

  9. Superior in vitro and in vivo activity of trastuzumab-emtansine (T-DM1) in comparison to trastuzumab, pertuzumab and their combination in epithelial ovarian carcinoma with high HER2/neu expression.

    Science.gov (United States)

    Menderes, Gulden; Bonazzoli, Elena; Bellone, Stefania; Altwerger, Gary; Black, Jonathan D; Dugan, Katherine; Pettinella, Francesca; Masserdotti, Alice; Riccio, Francesco; Bianchi, Anna; Zammataro, Luca; de Haydu, Christopher; Buza, Natalia; Hui, Pei; Wong, Serena; Huang, Gloria S; Litkouhi, Babak; Ratner, Elena; Silasi, Dan-Arin; Azodi, Masoud; Schwartz, Peter E; Santin, Alessandro D

    2017-10-01

    Epithelial ovarian cancer (EOC) remains the most lethal gynecologic malignancy. The objective of this study was to compare the anti-tumor activity of HER2/neu-targeting monoclonal antibodies, trastuzumab (T), pertuzumab (P), combination of trastuzumab and pertuzumab (T+P) and trastuzumab-emtansine (T-DM1) in EOC with high HER2/neu expression. Primary EOC cell lines were established and cell blocks were analyzed for HER2/neu expression. Cytostatic, apoptotic and antibody-dependent cell-mediated cytotoxicity (ADCC) activities of T, P, T+P and T-DM1 were evaluated in vitro. The in vivo antitumor activity was tested in xenograft models with 3+ HER2/neu expression. High (3+) HER2/neu expression was detected in 40% of the primary EOC cell lines. T, P, T+P, and T-DM1 were similarly effective in inducing strong ADCC against primary EOC cell lines expressing 3+ HER2/neu. The combination of T and P was more cytostatic when compared with that of T or P used alone (pT-DM1 induced significantly more apoptosis when compared with T+P (pT-DM1 was significantly more effective in tumor growth inhibition in vivo in EOC xenografts overexpressing HER2/neu when compared to T alone, P alone and T+P (p=0.04). In vitro and in vivo experiments with 3+ HER2/neu expressing EOC revealed limited anti-tumor activity of T or P. T-DM1 showed superior anti-tumor activity to T and P as single agents and as a combination. Our preclinical data support the design of clinical studies with T-DM1 for the treatment of chemotherapy-resistant EOC overexpressing HER2/neu. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media Aspectos biológicos do clone Dm 28c de Trypanosoma cruzi após metaciclogênese em meio quimicamente definido

    Directory of Open Access Journals (Sweden)

    Victor T. Contreras

    1988-03-01

    Full Text Available The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media. In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.A caracterização biológica do clone Dm 28c de Trypanosoma cruzi em termos do seu crescimento em meio LIT, ciclo celular, infectividade para camundongos e interação com células fagocíticas profissionais e não-profissionais, mostra que o mesmo comporta-se como um fiel representante da espécie T. cruzi. As propriedades biológicas deste clone miotrópico não mudam de acordo com a proveniência das formas tripomastigotas (i. e., de triatomíneos, de camundongos infectados, de cultura celular ou dos meios quimicamente definidos TAUP e TAU3AAG. Ainda mais, formas tripomastigotas metacíclicas do clone Dm 28c derivado do meio TAU3AAG apresentam um alto grau de infectividade para fibroblastos e células de músculo. Experimentos de ligação de ferritina cationizada à superfície de

  11. SP-1000i全自动推片染色仪及CellaVision DM96自动阅片仪在形态学检验人员镜下比对中的应用%Application of Morphology Microscopically Comparison by Using SP-1000i and CellaVision DM96

    Institute of Scientific and Technical Information of China (English)

    刘艳红

    2016-01-01

    Objective To improve the objectivity and consistency of microscopically comparison in morphology by using SP-1000i and CellaVision DM96.Methods Ten cases of venous blood samples were collected.The morphological comparison of white blood cells were performed between the traditional method and the new method (by using SP-1000i and CellaVision DM96),at the same time,between 2 veterans and other 10 common operators.According to the same methods,the time was compared.Results By using this new method,the consistency of microscopically comparison was greatly improved (P<0.01),the number of people who need to compare again was decreased obviously(P<0.01),and the time of the procedure was also shortened evidently(P<0.05).Conclusion The quality control of clinic laboratory will must be greatly improved with timesaving and labour-saving when SP-1000i and CellaVision DM96 are applied in microscopically comparison in mor-phology.%目的将 SP-1000i全自动推片染色仪及CellaVision DM96自动阅片仪应用于形态学镜下人员比对工作以提高客观性和一致性。方法实验室两名有经验的员工及其他10名值班人员分别按照传统镜检方法和新比对方法(使用 SP-1000i全自动推片染色仪及CellaVision DM96自动阅片仪)对10例静脉血样本进行白细胞分类形态学比对,并比较两种比对方法所需的时间。结果采用新比对方法较传统方法能够明显提高人员间对各类细胞分类的一致性(P<0.01),大大降低了需重新比对的人数(P<0.01),明显缩短了参比人员所花费的时间(P<0.01)。结论将 SP-1000i和 CellaVision DM96应用于细胞形态学镜下人员比对工作,可以省时、省力、高效地提高实验室质量管理。

  12. Technology Innovation Of Organic Waste Decomposition In Providing Feedstuffs

    Directory of Open Access Journals (Sweden)

    S. Prawirodigdo

    2011-06-01

    Full Text Available Previous investigations in Indonesia indicated that an inactive ovary was a chronically reproduction problem in ruminants. There was a tendency that nutrition deficiency inhibited ovulation, oestrus occurrence, and conception in ruminants. Obviously, there is a correlation between sufficient nutrient consumption and reproduction performance of such animals. Thus, application of the production/reproduction technology innovation for improving ruminant’s productivity in the villages needs to be supported by the availability of sufficient feed. Whilst, there is a competition among ruminants in fulfilling feed requirement. On the other hand, there are large amounts of organic waste of food and plantation estate industries which are potential for non-traditional feedstuffs. The examples of such organic wastes are: 4,817,630 ton dry matter (DM of cacao pod, 314,042.51 ton DM of coffee pulp and hulls, and 29,700,000 ton DM of palm frond, leaves and trunks. Unfortunately, such materials contain anti-nutritive substance. Nevertheless, technology innovation for decomposing organic waste is available and its validity has been proven to be satisfactory and appropriate. Regarding the limitation of feedstuffs, introduction of technology innovation for organic waste decomposition to provide feed for improving livestock productivity is promising to be applied.

  13. Study on the Separation and Purification of Elaeagnus Angustifolia Polysaccharide by Using the DM-18 Macroporous Resins%DM-18型大孔树脂分离纯化新疆沙枣果肉中多糖的工艺

    Institute of Scientific and Technical Information of China (English)

    杨魁; 陈晴晴; 杨金凤; 杨静; 陈景堃; 刘红

    2014-01-01

    To research and optimize the best conditions of the DM-18 macroporous resins,a dynamic absorption and desorption experiment was designed.The result showed that the best conditions as follows:2.0 mg /mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide,the sample loading amount of 3.0 BV,1.5 BV/h of the rate,the eluent concentration of 35%,the total quantity of eluent of 4.0 BV and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.13% and 92.17% under the conditions above.It concludes that the DM-18 macroporous resins is a good material to separae and purificate the Elaeagnus angustifolia polysaccharide.%本文研究了DM-18型大孔树脂分离纯化沙枣多糖的工艺条件,考察了各因素对分离、纯化沙枣多糖效果的影响,确定了分离沙枣多糖的最佳分离条件。结果表明:在沙枣多糖样品溶液2.0 mg /mL,上样速率为1.5 BV/h,上样液pH 值为7.0,上样量为3.0 BV、洗脱剂乙醇浓度为35%、洗脱剂用量为4.0 BV、洗脱速率为1.0 BV/h时,DM-18型大孔树脂对沙枣多糖的动态吸附率和解吸率分别达到90.13%和92.17%,表明该大孔树脂是一种较好的分离纯化沙枣多糖的材料。

  14. « Et ils ne vécurent pas heureux » : la fin de Jane Eyre réécrite dans Charlotte de D.M. Thomas “And they didn’t live happily ever after”: D.M. Thomas’s Rewriting of the Ending of Jane Eyre in Charlotte

    Directory of Open Access Journals (Sweden)

    Armelle Parey

    2009-10-01

    Full Text Available D.M. Thomas’s rewriting of Jane Eyre in Charlotte (2000 takes the shape of a transformation of the ending which enables the second Mrs Rochester’s story to be continued in the West Indies, thus also acknowledging Jean Rhys’s Wide Sargasso Sea as another intertext. In order to challenge the narrative and ideological hierarchy of the source text by rewriting its ending, the pastiche defamiliarises it while some chapters set in 1999 move the limits of rewriting and take the reader into a narrative spiral.

  15. Association of shortened telomere length with oxidative stress in leukocytes of T1DM and T2DM patients%1型及2型糖尿病患者外周血白细胞端粒长度减短与氧化应激关系的研究

    Institute of Scientific and Technical Information of China (English)

    杨雁; 胡鹏; 刘喆隆; 严江涛; 张晓洁; 袁刚

    2013-01-01

    Objective To analyze the severity of DNA damage by oxidative stress in diabetic patients and the correlation of diabetic oxidative stress with the changes of telomere length > and to explore the causes of shortened telomere length in diabetic condition. Methods T2DM patients (n = 62), T1DM patients (n=34), and non-diabetic subjects (n = 41), were enrolled in this trial. The leukocytes of peripheral blood of all subjects were obtained for detecting the 8-hydroxyl deoxyguanosine (8-OHdG) by ELISA. By using a quantitative PCR assay, the telomere length was determined and the average telomere size in leukocyte DNA was measured. Results The 8-OHdG level of T2DM group (2. 97 ±0. 85) and T1DM group (1. 97±0. 87) was significantly higher than that of the control group (0. 90 ±0. 44). In comparing the telomere length of T2DM group (1. 67 ± 0. 50) and T1DM group (1. 47±0. 50) with the control group (2. 39±0. 55), that of the former two groups was significantly shortened than that of the later group. In the multivariate linear regression analysis, the age and 8-OHdG level were negatively correlated with telomere length (P=0.001, β=-0.018; P=0. 023, β=-0.158) in T2DM group, but in the T1DM group, only the 8-OHdG level was negatively correlated with telomere length (P=0. 001, β= -0. 358). Conclusion The telomere length is significantly shortened in leukocytes of diabetic patients, and the shortening in the telomere of the T1DM patients is more obvious. The telomere shortening in diabetic condition is mainly associated with the oxidative stress induced by hyperglycemia, age, and the level of insulin secretion.%目的 分析糖尿病氧化应激对DNA损伤程度与端粒长度的相关性,探讨糖尿病时端粒减短的原因. 方法 选取T2DM患者(T2DM组)62例,T1DM患者(T1DM组)34例,对照(NC)组41名,提取外周血白细胞,ELISA法检测白细胞中8羟基脱氧鸟苷(8-OHdG),定量PCR检测端粒长度.结果 8-OHdG水平T2DM组(2.97±0.85)与T1DM组(1.97±0

  16. 非硅型氧漂稳定剂DM-1404的性能及应用%Application and Property of Non-Silicone Oxidation Bleach Stabilizer DMo1404

    Institute of Scientific and Technical Information of China (English)

    陈辉丰; 周秋宝

    2011-01-01

    The properties of non-silicone oxidation bleach stabilizer DM-1404 on the stabilization of hydro-gen peroxide and alkali-resistant and anti-fouling are studied. Application of non-Silicone stabilizer DM-1404 for the cotton fabric dip bleaching, cold pad-bath bleaching and pad-steam process is tested, which was compared with Na2SiO3. The experiment result exhibited that DM- 1404 has many advantages such as rendering fabric better whiteness, capillary effect and hand feeling, its anti-fouling ability is superior to Na2SiO3.%研究了非硅型氧漂稳定剂DM-1404的耐碱性、防污垢性及对双氧水的稳定作用,并试验了稳定剂DM-1404在棉织物浸漂、冷轧堆、汽蒸工艺中的应用效果,并与硅酸钠作了比较。结果表明,该双氧水稳定剂对双氧水有良好的稳定作用,防污垢能力优于硅酸钠,能使织物获得较好的白度、毛效和手感。

  17. Size, density and cholesterol load of HDL predict microangiopathy, coronary artery disease and β-cell function in men with T2DM.

    Science.gov (United States)

    Hermans, Michel P; Amoussou-Guenou, K Daniel; Bouenizabila, Evariste; Sadikot, Shaukat S; Ahn, Sylvie A; Rousseau, Michel F

    2016-09-01

    The role of high-density lipoprotein cholesterol (HDL-C) as modifiable risk factor for cardiovascular (CV) disease is increasingly debated, notwithstanding the finding that small-dense and dysfunctional HDL are associated with the metabolic syndrome and T2DM. In order to better clarify the epidemiological risk related to HDL of different size/density, without resorting to direct measures, it would seem appropriate to adjust HDL-C to the level of its main apolipoprotein (apoA-I), thereby providing an [HDL-C/apoA-I] ratio. The latter allows not only to estimate an average size for HDLs, but also to derive indices on particle number, cholesterol load, and density. So far, the potential usefulness of this ratio in diabetes is barely addressed. To this end, we sorted 488 male patients with T2DM according to [HDL-C/apoA-I] quartiles (Q), to determine how the ratio relates to cardiometabolic risk, β-cell function, glycaemic control, and micro- and macrovascular complications. Five lipid parameters were derived from the combined determination of HDL-C and apoA-I, namely HDL size; particle number; cholesterol load/particle; apoA-I/particle; and particle density. An unfavorable cardiometabolic profile characterized patients from QI and QII, in which HDLs were pro-atherogenic, denser and apoA-I-depleted. By contrast, QIII patients had an [HDL-C/apoA-I] ratio close to that of non-diabetic controls. QIV patients had better than average HDL size and composition, and in those patients whose [HDL-C/apoA-I] ratio was above normal, a more favorable phenotype was observed regarding lifestyle, anthropometry, metabolic comorbidities, insulin sensitivity, MetS score/severity, glycaemic control, and target-organ damage pregalence in small or large vessels. In conclusion, [HDL-C/apoA-I] and the resulting indices of HDL composition and functionality predict macrovascular risk and β-cell function decline, as well as overall microangiopathic risk, suggesting that this ratio could serve

  18. Hydrocarbon production and reservoir management: recent advances in closed-loop optimization technology

    NARCIS (Netherlands)

    Abbink, O.A.; Hanea, R.G.; Nennie, E.D.; Peters, R.C.A.M.

    2009-01-01

    Petroleum production is a relatively inefficient process. For oil production, it is, generally, less than 60 % effective on a macro scale and less than 60 % effective on a micro scale. This results, commonly, in an actual oil recovery of less than 35 %. Optimization of the production process will, t

  19. Development of Nanotools for Applications in (sub-)Femtofluidics and Graphene Technologies

    NARCIS (Netherlands)

    Perez Garza, H.H.

    2015-01-01

    Properties of matter at the nano-scale may be very different from the ones observed at the macro-scale. The continuous variation of characteristics with diminishing size results in relevant changes in behavior. Similarly, these changes are caused by the rise of completely new phenomena (i.e. quantum

  20. Development of Nanotools for Applications in (sub-)Femtofluidics and Graphene Technologies

    NARCIS (Netherlands)

    Perez Garza, H.H.

    2015-01-01

    Properties of matter at the nano-scale may be very different from the ones observed at the macro-scale. The continuous variation of characteristics with diminishing size results in relevant changes in behavior. Similarly, these changes are caused by the rise of completely new phenomena (i.e. quantum

  1. Technology Maturity is Technology Superiority

    Science.gov (United States)

    2008-09-09

    Dominant Air Power: Design For Tomorrow…Deliver Today 2 TECHNOLOGY MATURITY CONFERENCE • ONE DEFINITION OF MATURITY – GOOD JUDGEMENT COMES FROM...EXPERIENCE—EXPERIENCE COMES FROM BAD JUDGEMENT Dominant Air Power: Design For Tomorrow…Deliver Today 3 TECHNOLOGY MATURITY CONFERENCE • THIS WILL BE A...2008 TECHNOLOGY MATURITY CONFERENCE “ TECHNOLOGY MATURITY IS TECHNOLOGY SUPERIORITY” Aeronautical Systems Center Dr. Tom Christian ASC/EN, WPAFB OH

  2. The effects of the RNA in Lactobacillus DM9811 ferment culture on cAMP/cGMP in HELA cell%乳杆菌DM9811代谢产物中的RNA组分对宫颈癌细胞cAMP/cGMP影响

    Institute of Scientific and Technical Information of China (English)

    汪剑萍; 唐立

    2007-01-01

    目的 通过分析乳杆菌DM9811代谢产物中的RNA组分对宫颈癌细胞cAMP/cGMP影响,探讨乳杆菌对细胞作用的分子机制.方法 采用细胞与分子生物学方法.结果 乳杆菌代谢产物中的RNA组分对细胞的信息分子cAMP具有重要的调节活性.当除去代谢产物中的RNA分子刺激细胞时,细胞内的cAMP水平低于纯化后的RNA组cAMP水平,低了1.75倍,cGMP水平也出现了对应关系.结论 乳杆菌DM9811代谢产物中的RNA组分对细胞内信息分子cAMP/cGMP具有调节作用.

  3. Dark Matter as a Possible New Energy Source for Future Rocket Technology

    CERN Document Server

    Liu, Jia

    2009-01-01

    Current rocket technology can not send the spaceship very far, because the amount of the chemical fuel it can take is limited. We try to use dark matter (DM) as fuel to solve this problem. In this work, we give an example of DM engine using dark matter annihilation products as propulsion. The acceleration is proportional to the velocity, which makes the velocity increase exponentially with time in non-relativistic region. The important points for the acceleration are how dense is the DM density and how large is the saturation region. The parameters of the spaceship may also have great influence on the results. We show that the (sub)halos can accelerate the spaceship to velocity $ 10^{- 5} c \\sim 10^{- 3} c$. Moreover, in case there is a central black hole in the halo, like the galactic center, the radius of the dense spike can be large enough to accelerate the spaceship close to the speed of light.

  4. The Role of DmCatD, a Cathepsin D-Like Peptidase, and Acid Phosphatase in the Process of Follicular Atresia in Dipetalogaster maxima (Hemiptera: Reduviidae), a Vector of Chagas' Disease

    Science.gov (United States)

    Leyria, Jimena; Fruttero, Leonardo L.; Nazar, Magalí; Canavoso, Lilián E.

    2015-01-01

    In this work, we have investigated the involvement of DmCatD, a cathepsin D-like peptidase, and acid phosphatase in the process of follicular atresia of Dipetalogaster maxima, a hematophagous insect vector of Chagas’ disease. For the studies, fat bodies, ovaries and hemolymph were sampled from anautogenous females at representative days of the reproductive cycle: pre-vitellogenesis, vitellogenesis as well as early and late atresia. Real time PCR (qPCR) and western blot assays showed that DmCatD was expressed in fat bodies and ovaries at all reproductive stages, being the expression of its active form significantly higher at the atretic stages. In hemolymph samples, only the immunoreactive band compatible with pro-DmCatD was observed by western blot. Acid phosphatase activity in ovarian tissues significantly increased during follicular atresia in comparison to pre-vitellogenesis and vitellogenesis. A further enzyme characterization with inhibitors showed that the high levels of acid phosphatase activity in atretic ovaries corresponded mainly to a tyrosine phosphatase. Immunofluorescence assays demonstrated that DmCatD and tyrosine phosphatase were associated with yolk bodies in vitellogenic follicles, while in atretic stages they displayed a different cellular distribution. DmCatD and tyrosine phosphatase partially co-localized with vitellin. Moreover, their interaction was supported by FRET analysis. In vitro assays using homogenates of atretic ovaries as the enzyme source and enzyme inhibitors demonstrated that DmCatD, together with a tyrosine phosphatase, were necessary to promote the degradation of vitellin. Taken together, the results strongly suggested that both acid hydrolases play a central role in early vitellin proteolysis during the process of follicular atresia. PMID:26091289

  5. A retrospective analysis of a societal experiment among the Danish population suggests that exposure to extra doses of vitamin A during fetal development may lower type 2 diabetes mellitus (T2DM) risk later in life.

    Science.gov (United States)

    Keller, Amélie; Ängquist, Lars; Jacobsen, Ramune; Vaag, Allan; Heitmann, Berit L

    2017-03-01

    Vitamin A deficiency has been associated with impaired fetal pancreatic development and increased risk of developing type 2 diabetes mellitus (T2DM). In 1962, mandatory margarine fortification with vitamin A was increased by 25 % in Denmark. We aimed to determine whether offspring of mothers who had been exposed to the extra vitamin A from fortification during pregnancy had a lower risk of developing T2DM in adult life, compared with offspring of mothers exposed to less vitamin A. Individuals from birth cohorts with the higher prenatal vitamin A exposure (born 1 December 1962-31 March 1964) and those with lower prenatal exposure (born 1 September 1959-31 December 1960) were followed up with regard to development of T2DM before 31 December 2012 in the Danish National Diabetes Registry and National Patient Register. Logistic and Cox regression analyses were performed to determine the risk of T2DM by vitamin A exposure level. A total of 193 803 individuals were followed up until midlife. Our results showed that individuals exposed prenatally to extra vitamin A from fortified margarine had a lower risk of developing T2DM than those exposed to lower levels: OR 0·88; 95 % CI 0·81, 0·95, P=0·001, after adjustment for sex. Fetal exposure to small, extra amounts of vitamin A from food fortification may reduce the risk of T2DM. These results may have public health relevance, as they demonstrate that one of the most costly chronic diseases may be prevented by food fortification - a simple and affordable public health nutrition intervention.

  6. Effects of Clear Kefir on Biomolecular Aspects of Glycemic Status of Type 2 Diabetes Mellitus (T2DM Patients in Bandung, West Java [Study on Human Blood Glucose, c Peptide and Insulin

    Directory of Open Access Journals (Sweden)

    Judiono J

    2014-08-01

    Full Text Available Background: Diabetes Mellitus (DM triggers an excessive reaction of free-radicals. It increases reactive oxygen species and reduces antioxidants status as well as the β cell damage. Clear kefir was used for DM therapies, however it limited biomolecular exploration of its bioactive roles. Research aimed to investigate the effects of clear kefir on the biomolecular nature of the glycemic status of T2DM in Bandung. Methods: The randomized pretest-posttest control group was conducted by 106 T2DM patients. Research was done in several hospitals in Bandung and Cimahi, West Java from 2012–2013. Samples were divided randomly into three groups: (1 T2DM with HbA1c 7 fed standard diet and supplemented 200 ml/day by clear kefir, (3 T2DM with HbA1c was fed a standard diet as a control group. Dose response was obtained from a preeliminary vivo study, and then converted to human dosage by year 2011. Intervention was effectively done for 30 days. HbA1c was measured by HPLC. Fasting blood glucose (FBG and Postprandial blood glucose levels (PBG were measured by enzymes levels. C Peptide and insulin were measured by Elisa. Data was analyzed by a statictics programme by significance p<0,05. Study was approved by ethic committee. Results : HbA1c was significantly reduced in delta level (p<0.01 and FBG (p<0.015 among kefir groups. PBG was not significantly reduced among groups. C-Peptide was significantly increased in delta level, except in control group (p<0.014. Insulin was reduced significantly, except in control group (p<0.003. Conclusions : Supplementation of clear kefir reduced blood glucose levels (HbA1c, FBG, PBG and increased c-peptide. Clear kefir’s biomolecular mechanisms and chemistry characterization is a challenge for future studies.

  7. A STUDY ON PREVALENCE OF DM, HYPERTENSION AND ASSOCIATION WITH LIFE STYLE AS RISK FACTORS IN A RURAL POPULATION DISTRICT GHAZIABAD (U. P.

    Directory of Open Access Journals (Sweden)

    Narendra

    2014-12-01

    Full Text Available BACKGROUND & OBJECTIVE: Estimation of rising prevalence of Diabetes Mellitus, Hypertension, Obesity etc & association with various risk factors is having bearing on effective preventive programmes. Cross sectional study was conducted in rural population in a health centre to assess prevalence of DM, Hypertension, and association with their life style if any. METHODS: Pretested proforma administered to all males 35 -50 yrs age coming to a health Centre. Information on diets, habits, physical activity, medical & family history, height, weight, blood pressure, blood sugar level was taken. For data analyses epi info software used. RESULTS: Out of 1120 participants, 186 were having Hypertension, prevalence of 16.87%, 14 hypertensive, (7.53 % with positive family history of hypertension. 89 were Diabetic, prevalence of 7.94 %. In this group, 7 (7.90% had family history of diabetes. Overall 258 (25.03% had abnormal BMI, age group with highest BMI as risk factor, had higher hypertensive & Diabetics persons, most of them consuming non-vegetarian diet with saturated fats. Most of participants were doing mild to moderate physical activity. INTERPRETATION & CONCLUSIONS: The study provides insight on high burden, of Hypertension, Diabetes Mellitus, and the associated risk factors in a rural population in a health Centre. Life style modifications, more physical activity, lesser intake of non-vegetarian items, fats, salt in the diet, will result in lesser risk & load of these diseases.

  8. CID: Chemistry in disks VI.sulfur-bearing molecules in the protoplanetary disks surrounding LkCa15, MWC480, DM Tau, and GO Tau

    CERN Document Server

    Dutrey, Anne; Boehler, Yann; Guilloteau, Stéphane; Hersant, Franck; Semenov, Dmitry; Chapillon, Edwige; Henning, Thomas; Piétu, Vincent; Launhardt, Ralf; Gueth, Frederic; Schreyer, Katharina

    2011-01-01

    We study the content in S-bearing molecules of protoplanetary disks around low-mass stars. We used the new IRAM 30-m receiver EMIR to perform simultaneous observations of the $1_{10}-1_{01}$ line of H$_2$S at 168.8 GHz and $2_{23}-1_{12}$ line of SO at 99.3 GHz. We compared the observational results with predictions coming from the astrochemical code NAUTILUS, which has been adapted to protoplanetary disks. The data were analyzed together with existing CS J=3-2 observations. We fail to detect the SO and H$_2$S lines, although CS is detected in LkCa15, DM\\,Tau, and GO\\,Tau but not in MWC\\,480. However, our new upper limits are significantly better than previous ones and allow us to put some interesting constraints on the sulfur chemistry. Our best modeling of disks is obtained for a C/O ratio of 1.2, starting from initial cloud conditions of H density of $2\\times 10^5$ cm$^{-3}$ and age of $10^6$ yr. The results agree with the CS data and are compatible with the SO upper limits, but fail to reproduce the H$_2$...

  9. Effects of lorcaserin on fat and lean mass loss in obese and overweight patients without and with type 2 diabetes mellitus: the BLOSSOM and BLOOM-DM studies.

    Science.gov (United States)

    Apovian, C; Palmer, K; Fain, R; Perdomo, C; Rubino, D

    2016-09-01

    Body composition was determined using dual-energy X-ray absorptiometry (DXA) in a subset of patients without (BLOSSOM) and with (BLOOM-DM) type 2 diabetes who received diet and exercise counselling along with either lorcaserin 10 mg twice daily or placebo. DXA scans were performed on study day 1 (baseline), week 24 and week 52. Baseline demographics of the subpopulations (without diabetes, n = 189; with diabetes, n = 63) were similar between studies and representative of their study populations. At week 52, patients without diabetes on lorcaserin lost significantly more fat mass relative to those on placebo (-12.06% vs -5.93%; p = 0.008). In patients with diabetes, fat mass was also decreased with lorcaserin relative to placebo (-9.87% vs -1.65%; p fat mass was lost in the trunk region with lorcaserin compared with placebo (without diabetes: -3.31% vs -2.05%; with diabetes: -3.65% vs -0.36%). Weight loss with lorcaserin was associated with a greater degree of fat mass loss than lean mass loss, and most of the fat mass lost for patients without and with diabetes was from the central region of the body. © 2016 John Wiley & Sons Ltd.

  10. Globalization & technology

    DEFF Research Database (Denmark)

    Narula, Rajneesh

    Technology and globalization are interdependent processes. Globalization has a fundamental influence on the creation and diffusion of technology, which, in turn, affects the interdependence of firms and locations. This volume examines the international aspect of this interdependence at two levels...

  11. Living Technology

    DEFF Research Database (Denmark)

    2010-01-01

    This book is aimed at anyone who is interested in learning more about living technology, whether coming from business, the government, policy centers, academia, or anywhere else. Its purpose is to help people to learn what living technology is, what it might develop into, and how it might impact...... our lives. The phrase 'living technology' was coined to refer to technology that is alive as well as technology that is useful because it shares the fundamental properties of living systems. In particular, the invention of this phrase was called for to describe the trend of our technology becoming...... increasingly life-like or literally alive. Still, the phrase has different interpretations depending on how one views what life is. This book presents nineteen perspectives on living technology. Taken together, the interviews convey the collective wisdom on living technology's power and promise, as well as its...

  12. Living Technology

    DEFF Research Database (Denmark)

    2010-01-01

    This book is aimed at anyone who is interested in learning more about living technology, whether coming from business, the government, policy centers, academia, or anywhere else. Its purpose is to help people to learn what living technology is, what it might develop into, and how it might impact...... our lives. The phrase 'living technology' was coined to refer to technology that is alive as well as technology that is useful because it shares the fundamental properties of living systems. In particular, the invention of this phrase was called for to describe the trend of our technology becoming...... increasingly life-like or literally alive. Still, the phrase has different interpretations depending on how one views what life is. This book presents nineteen perspectives on living technology. Taken together, the interviews convey the collective wisdom on living technology's power and promise, as well as its...

  13. Emerging Technologies

    OpenAIRE

    Salgar, S. M.

    2004-01-01

    Phenomenal advancements have taken place in the field of Information and communication technologies in the last decade. Spectacular and innovative changes are expected to take place in these fields in coming decade. Networking technologies are going through a sea change. This paper enumerates the likely networking technologies which are emerging, particularly WLANs. Most of the personal communication in the country will be through cellular/ mobile technologies, which are also covered in the p...

  14. Soulful Technologies

    DEFF Research Database (Denmark)

    Fausing, Bent

    2010-01-01

    or anthropomorphism is important for the branding of new technology. Technology is seen as creating a techno-transcendence towards a more qualified humanity which is in contact with fundamental human values like intuition, vision, and sensing; all the qualities that technology, industrialization, and rationalization...

  15. Technology Tiers

    DEFF Research Database (Denmark)

    Karlsson, Christer

    2015-01-01

    A technology tier is a level in a product system: final product, system, subsystem, component, or part. As a concept, it contrasts traditional “vertical” special technologies (for example, mechanics and electronics) and focuses “horizontal” feature technologies such as product characteristics...

  16. Technology Tiers

    DEFF Research Database (Denmark)

    Karlsson, Christer

    2015-01-01

    A technology tier is a level in a product system: final product, system, subsystem, component, or part. As a concept, it contrasts traditional “vertical” special technologies (for example, mechanics and electronics) and focuses “horizontal” feature technologies such as product characteristics...

  17. Assistive Technologies

    Science.gov (United States)

    Auat Cheein, Fernando A., Ed.

    2012-01-01

    This book offers the reader new achievements within the Assistive Technology field made by worldwide experts, covering aspects such as assistive technology focused on teaching and education, mobility, communication and social interactivity, among others. Each chapter included in this book covers one particular aspect of Assistive Technology that…

  18. SLIM, Short-pulse Technology for High Gradient Induction Accelerators

    Energy Technology Data Exchange (ETDEWEB)

    Arntz, Floyd; /Diversified Tech., Bedford; Kardo-Sysoev, A.; /Ioffe Phys. Tech. Inst.; Krasnykh, A.; /SLAC

    2008-12-16

    A novel short-pulse concept (SLIM) suited to a new generation of a high gradient induction particle accelerators is described herein. It applies advanced solid state semiconductor technology and modern microfabrication techniques to a coreless induction method of charged particle acceleration first proven on a macro scale in the 1960's. Because this approach avoids use of magnetic materials there is the prospect of such an accelerator working efficiently with accelerating pulses in the nanosecond range and, potentially, at megahertz pulse rates. The principal accelerator section is envisioned as a stack of coreless induction cells, the only active element within each being a single, extremely fast (subnanosecond) solid state opening switch: a Drift Step Recovery Diode (DSRD). Each coreless induction cell incorporates an electromagnetic pulse compressor in which inductive energy developed within a transmission-line feed structure over a period of tens of nanoseconds is diverted to the acceleration of the passing charge packet for a few nanoseconds by the abrupt opening of the DSRD switch. The duration of this accelerating output pulse--typically two-to-four nanoseconds--is precisely determined by a microfabricated pulse forming line connected to the cell. Because the accelerating pulse is only nanoseconds in duration, longitudinal accelerating gradients approaching 100 MeV per meter are believed to be achievable without inciting breakdown. Further benefits of this approach are that, (1) only a low voltage power supply is required to produce the high accelerating gradient, and, (2) since the DSRD switch is normally closed, voltage stress is limited to a few nanoseconds per period, hence the susceptibility to hostile environment conditions such as ionizing radiation, mismatch (e.g. in medical applications the peak beam current may be low), strong electromagnetic noise levels, etc is expected to be minimal. Finally, we observe the SLIM concept is not limited to

  19. Sensemaking technologies

    DEFF Research Database (Denmark)

    Madsen, Charlotte Øland

    Research scope: The scope of the project is to study technological implementation processes by using Weick's sensemaking concept (Weick, 1995). The purpose of using a social constructivist approach to investigate technological implementation processes is to find out how new technologies transform......, Orlikowski 2000). Viewing the use of technology as a process of enactment opens up for investigating the social processes of interpreting new technology into the organisation (Orlikowski 2000). The scope of the PhD project will therefore be to gain a deeper understanding of how the enactment of new...... & Brass, 1990; Kling 1991; Orlikowski 2000). It also demonstrates that technology is a flexible variable adapted to the organisation's needs, culture, climate and management philosophy, thus leading to different uses and outcomes of the same technology in different organisations (Barley 1986; 1990...

  20. Appropriate Technology as Indian Technology.

    Science.gov (United States)

    Barry, Tom

    1979-01-01

    Describes the mounting enthusiasm of Indian communities for appropriate technology as an inexpensive means of providing much needed energy and job opportunities. Describes the development of several appropriate technology projects, and the goals and activities of groups involved in utilizing low scale solar technology for economic development on…

  1. Design and implementation of financial analysis system with hospital based on techniques of OLAP+DM%基于OLAP+DM技术的医院财务分析系统的设计与实现

    Institute of Scientific and Technical Information of China (English)

    王萤; 徐琛

    2016-01-01

    In this paper, the success of the hospital′s data bank and affiliated hospitals effectively combine information base, establishment of a large integrated data resource pool, and after a series of data extraction, transformation and loading for the integration of resources to make effective preparations, and the eventual establishment of an effective financial analysis of the data warehouse, to solve the industry has been plagued by the problem of data islands. On this basis, but also through analysis and derive some out using OLAP and DM technology, the financial statements of a full range of multi-dimensional analysis, and for the reliability and value of data mining to some extent, will the results of the data analysis provided to the hospital′s management and decision-making. To systematically test shows that the system is reliable and authoritative in the data analysis and processing integration.%文中成功将医院的数据信息库与下属医院的信息库有效相结合,建立了庞大的整合数据资源池,并经过一系列的数据抽取、转换和加载为资源的整合做出了有效的准备工作,且最终建立了一个有效的财务分析数据仓库,解决了始终困扰行业的数据孤岛问题。在此基础上,还经过一些列的分析和推导,利用OLAP和DM技术,对财务报表进行了全方位多维度的分析,同时对于数据的可靠性和价值性进行一定程度上的数据挖掘,将对数据分析的结果提供给医院的管理层和决策层。对系统地测试表明,该系统在数据分析和处理整合上均具有可靠性和权威性。

  2. Refused derived fuels. Technical rules of new law 5 February 1998 concerning its production and utilization; Combustibile derivato dai rifiuti. Le nuove norme tecniche (DM 5 febbraio 98) riguardanti la sua produzione ed utilizzazione

    Energy Technology Data Exchange (ETDEWEB)

    Maldifassi, C.; Tenti, R. [Consorzio Quadrifoglio, Florence (Italy)

    1999-08-01

    Characteristics of Refused Derived Fuels, inferred from samples which represent significantly the production of some recent plants, are compared with requisites of the new law dated 5 February 1998 and those mentioned of the previous law of 16 January 1995 and with technical norm UNI 9903 rule. The results is that the 'non conformities' are very numerous, only 4% os samples is in accordance with the low 5 February 1998. Impositions of norms exceedingly strict and onerous as are those of the law 5 February 1998, highly obstructs this form of remake use of wastes, with adverse environment consequences to what the legislator is determined with the framework law in fact of wastes (5 February 1998). [Italian] Le caratteristiche del combustibile derivato dai rifiuti desunte dai campioni provenienti da alcuni impianti recenti, sono confrontate con i requisiti di cui al nuovo DM del 5 febbraio 98 con queli del precedente DM 16 gennaio 95 e con la norma UNI 9903. Le 'non conformita'' sono molto numerose, verso il DM 5 febbraio 98 e' conforme solo il 5% dei campioni. L'imposizione di norme eccessivamente severe ed onerose, come sono quelle del DM 5 febbraio 98, ostacola fortemente questa fora di riutilizzo dei rifiuti, con effetti ambientali contrari a quelli che il legislatore si prefigge con la norma 'quadro' in materia di rifiuti (D.Lgs. 5 febbraio 97, n.22).

  3. Living with diabetes: a group-based self-management support programme for T2DM patients in the early phases of illness and their partners, study protocol of a randomised comtrolled trial.

    NARCIS (Netherlands)

    Puffelen, A.L. van; Rijken, M.; Heijmans, M.J.W.M.; Nijpels, G.; Rutten, G.E.H.M.; Schellevis, F.G.

    2014-01-01

    Background: The present article presents the protocol for a randomised controlled trial to test the effectiveness of a group-based self-management support programme for recently diagnosed type 2 diabetes mellitus (T2DM) patients (one to three years post-diagnosis) and their partners. The course aims

  4. Risk factors of nonalcoholic fatty liver disease in patients with T2DM%2型糖尿病合并非酒精性脂肪肝的危险因素分析

    Institute of Scientific and Technical Information of China (English)

    张晓宇; 尹卓娜; 徐谷根

    2015-01-01

    ObjectiveTo explore the risk factors of nonalcoholic fatty liver disease (NAFLD) in patients with T2DM.Methods A total of 176 patients with T2DM in Department of Endocrinology in The Second People's Hospital of Guangdong Province from January 2013 to January 2014 were enrolled. T2DM was diagnosed by WHO standard and NAFLD was by guidelines for management of NAFLD. Results Body mass index, systolic blood pressure, heart rate, fasting insulin, postprandial blood glucose, homeostasis model assessment insulin resistance (HOMA-IR), uric, low density lipoprotein, Crea, alanine transaminase, aspartate aminotransferase, γ-glutamyl transpeptidase, intima-media thickness (IMT) of patients with NAFLD in T2DM group were significantly different, compared with those in non-NAFLD group (P50岁的2型糖尿病女性患者NAFLD患病率明显升高(P<0.05).结论 HOMA-IR、AST、右颈动脉分叉IMT是NAFLD的主要独立危险因素.

  5. Instrument for assessing mobile technology acceptability in diabetes self-management: a validation and reliability study

    Directory of Open Access Journals (Sweden)

    Frandes M

    2017-02-01

    Full Text Available Mirela Frandes,1 Anca V Deiac,2 Bogdan Timar,1,3 Diana Lungeanu1,2 1Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy of Timisoara, 2Department of Mathematics, Polytechnic University of Timisoara, 3Third Medical Clinic, Emergency Hospital of Timisoara, Timisoara, Romania Background: Nowadays, mobile technologies are part of everyday life, but the lack of instruments to assess their acceptability for the management of chronic diseases makes their actual adoption for this purpose slow.Objective: The objective of this study was to develop a survey instrument for assessing patients’ attitude toward and intention to use mobile technology for diabetes mellitus (DM self-management, as well as to identify sociodemographic characteristics and quality of life factors that affect them.Methods: We first conducted the documentation and instrument design phases, which were subsequently followed by the pilot study and instrument validation. Afterward, the instrument was administered 103 patients (median age: 37 years; range: 18–65 years diagnosed with type 1 or type 2 DM, who accepted to participate in the study. The reliability and construct validity were assessed by computing Cronbach’s alpha and using factor analysis, respectively.Results: The instrument included statements about the actual use of electronic devices for DM management, interaction between patient and physician, attitude toward using mobile technology, and quality of life evaluation. Cronbach’s alpha was 0.9 for attitude toward using mobile technology and 0.97 for attitude toward using mobile device applications for DM self-management. Younger patients (Spearman’s ρ=-0.429; P<0.001 with better glycemic control (Spearman’s ρ=-0.322; P<0.001 and higher education level (Kendall’s τ=0.51; P<0.001 had significantly more favorable attitude toward using mobile assistive applications for DM control. Moreover, patients with a higher quality of

  6. The pathogenesis of interstitial lung disease (ILD) associated with polymyositis (PM) and dermatomyositis (DM)%多发性肌炎(PM)/皮肌炎(DM)相关的肺间质病变发(ILD)病机制的研究进展

    Institute of Scientific and Technical Information of China (English)

    张立

    2015-01-01

    肺间质病变(interstitial lung disease,ILD)是多发性肌炎(polymyositis,PM)/皮肌炎(dermatomyositis,DM)的常见并发症,预后不良且死亡率高,是PM/DM患者住院和死亡的重要原因.PM/DM相关ILD发病机制目前仍不清楚;治疗上仍以激素为主,尚缺乏有效的治疗方法,近年来,对ILD的发病机制和治疗已成为一个研究热点.本文就参与发病的自身抗体、免疫细胞、细胞因子、组织蛋白酶、遗传因素学等方面对PM/DM相关的ILD的发病机制研究作一简要综述.

  7. Onsite Greywater Treatment using Pilot Scale Technology

    Directory of Open Access Journals (Sweden)

    Manzoor-ul-Haq Rajput

    2015-04-01

    Full Text Available The GROW Technology for greywater treatment was installed at the MUET (Mehran University of Engineering & Technology, hostel and run under continuous flow conditions with hydraulic loading rate of 0.15m.d-1. The monitoring and analysis of influent and effluent water were carried out during January-December, 2010. Local plants species such as water hyacinth, Pennywort (duck weed, Mint and Cattail were used in the GROW rig as a mixed mode. Coarse Gravels were filled in the troughs as a medium. The collected samples were analyzed for BOD5 (Biochemical Oxygen Demand, COD (Chemical Oxygen Demand, TSS (Total Suspended Solids, pH, and DO (Dissolved Oxygen. Removal efficiencies of BOD5, COD and TSS were calculated as 83.0,69.0 and 84.0% respectively. DO was found increased from 0.6-3.5 mg.dm-3 while pH was observed between 6.5-7.8

  8. Implementation of a 220,000-compound HCS campaign to identify disruptors of the interaction between p53 and hDM2 and characterization of the confirmed hits.

    Science.gov (United States)

    Dudgeon, Drew D; Shinde, Sunita; Hua, Yun; Shun, Tong Ying; Lazo, John S; Strock, Christopher J; Giuliano, Kenneth A; Taylor, D Lansing; Johnston, Patricia A; Johnston, Paul A

    2010-08-01

    In recent years, advances in structure-based drug design and the development of an impressive variety of high-throughput screening (HTS) assay formats have yielded an expanding list of protein-protein interaction inhibitors. Despite these advances, protein-protein interaction targets are still widely considered difficult to disrupt with small molecules. The authors present here the results from screening 220,017 compounds from the National Institute of Health's small-molecule library in a novel p53-hDM2 protein-protein interaction biosensor (PPIB) assay. The p53-hDM2 positional biosensor performed robustly and reproducibly throughout the high-content screening (HCS) campaign, and analysis of the multiparameter data from images of the 3 fluorescent channels enabled the authors to identify and eliminate compounds that were cytotoxic or fluorescent artifacts. The HCS campaign yielded 3 structurally related methylbenzo-naphthyridin-5-amine (MBNA) hits with IC(50)s between 30 and 50 microM in the p53-hDM2 PPIB. In HCT116 cells with wild-type (WT) p53, the MBNAs enhanced p53 protein levels, increased the expression of p53 target genes, caused a cell cycle arrest in G1, induced apoptosis, and inhibited cell proliferation with an IC(50) ~4 microM. The prototype disruptor of p53-hDM2 interactions Nutlin-3 was more potent than the MBNAs in the p53-hDM2 PPIB assay but produced equivalent biological results in HCT116 cells WT for p53. Unlike Nutlin-3, however, MBNAs also increased the percentage of apoptosis in p53 null cells and exhibited similar potencies for growth inhibition in isogenic cell lines null for p53 or p21. Neither the MBNAs nor Nutin-3 caused cell cycle arrest in p53 null HCT116 cells. Despite the relatively modest size of the screening library, the combination of a novel p53-hDM2 PPIB assay together with an automated imaging HCS platform and image analysis methods enabled the discovery of a novel chemotype series that disrupts p53-hDM2 interactions in cells.

  9. Effect of metformin combined with Liuwei Dihuang pill on serum illness-related molecule con-tents in T2DM patients with NAFLD%二甲双胍联用六味地黄丸对 T2DM 并 NAFLD 患者血清病情相关分子含量的影响

    Institute of Scientific and Technical Information of China (English)

    郑仲萍; 田峰; 郑志刚; 张国庆; 郭锋; 郭杏花

    2016-01-01

    Objective:To study the effect of metformin combined with Liuwei Dihuang pill on serum illness-related mol-ecule contents in type 2 diabetes mellitus (T2DM)patients with non-alcoholic fatty liver disease (NAFLD).Methods:A total of 86 cases of newly diagnosed T2DM patients with NAFLD were selected for study and randomly divided into combination therapy group and monotherapy group who received metformin combined with Liuwei Dihuang pill therapy and metformin monotherapy respectively,and then serum glycolipid metabolism indexes,liver function indexes as well as oxidative stress re-sponse and inflammatory response indexes of two groups were detected.Results:(1 )Glycolipid metabolism indexes:fasting plasma glucose (FPG),2-h plasma glucose (2hPG),haemoglobin A1c (HbA1c),mean amplitude of glycemic excursion (MAGE),HOMA-IR,triglyceride (TG),total cholesterol (TC)and low-density lipoprotein cholesterol (LDL-C)as well as body mass index (BMI)and waist hip ratio of combination therapy group were lower than those of monotherapy group (P <0.05),and high-density lipoprotein cholesterol (HDL-C)content was significantly higher than that of monotherapy group (P <0.05);(2)liver function indexes:aspartate transaminase (AST),alanine aminotransferase (ALT),γ-glutamyl transpeptidase (γ-GT),serum total bilirubin (STB)and cholyglycine (CG)contents of combination therapy group were significantly lower than those of monotherapy group (P <0.05);(3)oxidative stress response and inflammatory response indexes:malondialde-hyde (MDA),advanced oxidation protein products (AOPP),tumor necrosis factor-α(TNF-α)and interleukin (IL)-6 contents of combination therapy group were significantly lower than those of monotherapy group (P < 0.05 ).Conclusion:Metformin combined with Liuwei Dihuang pill helps to regulate glycolipid metabolism,reduce liver function damage and alleviate oxidative stress response and inflammatory response in T2DM patients with NAFLD.%目的::研究二甲

  10. Sensemaking technologies

    DEFF Research Database (Denmark)

    Madsen, Charlotte Øland

    & Brass, 1990; Kling 1991; Orlikowski 2000). It also demonstrates that technology is a flexible variable adapted to the organisation's needs, culture, climate and management philosophy, thus leading to different uses and outcomes of the same technology in different organisations (Barley 1986; 1990......, Orlikowski 2000). Viewing the use of technology as a process of enactment opens up for investigating the social processes of interpreting new technology into the organisation (Orlikowski 2000). The scope of the PhD project will therefore be to gain a deeper understanding of how the enactment of new...

  11. Chemistry Technology

    Data.gov (United States)

    Federal Laboratory Consortium — Chemistry technology experts at NCATS engage in a variety of innovative translational research activities, including:Design of bioactive small molecules.Development...

  12. Instrument for assessing mobile technology acceptability in diabetes self-management: a validation and reliability study

    Science.gov (United States)

    Frandes, Mirela; Deiac, Anca V; Timar, Bogdan; Lungeanu, Diana

    2017-01-01

    Background Nowadays, mobile technologies are part of everyday life, but the lack of instruments to assess their acceptability for the management of chronic diseases makes their actual adoption for this purpose slow. Objective The objective of this study was to develop a survey instrument for assessing patients’ attitude toward and intention to use mobile technology for diabetes mellitus (DM) self-management, as well as to identify sociodemographic characteristics and quality of life factors that affect them. Methods We first conducted the documentation and instrument design phases, which were subsequently followed by the pilot study and instrument validation. Afterward, the instrument was administered 103 patients (median age: 37 years; range: 18–65 years) diagnosed with type 1 or type 2 DM, who accepted to participate in the study. The reliability and construct validity were assessed by computing Cronbach’s alpha and using factor analysis, respectively. Results The instrument included statements about the actual use of electronic devices for DM management, interaction between patient and physician, attitude toward using mobile technology, and quality of life evaluation. Cronbach’s alpha was 0.9 for attitude toward using mobile technology and 0.97 for attitude toward using mobile device applications for DM self-management. Younger patients (Spearman’s ρ=−0.429; P<0.001) with better glycemic control (Spearman’s ρ=−0.322; P<0.001) and higher education level (Kendall’s τ=0.51; P<0.001) had significantly more favorable attitude toward using mobile assistive applications for DM control. Moreover, patients with a higher quality of life presented a significantly more positive attitude toward using modern technology (Spearman’s ρ=0.466; P<0.001). Conclusion The instrument showed good reliability and internal consistency, making it suitable for measuring the acceptability of mobile technology for DM self-management. Additionally, we found that even

  13. FINAL REPORT INTEGRATED DM1200 MELTER TESTING OF BUBBLER CONFIGURATIONS USING HLW AZ-101 SIMULANTS VSL-04R4800-4 REV 0 10/5/04

    Energy Technology Data Exchange (ETDEWEB)

    KRUGER AA; MATLACK KS; GONG W; BARDAKCI T; D' ANGELO NA; LUTZE W; CALLOW RA; BRANDYS M; KOT WK; PEGG IL

    2011-12-29

    This report documents melter and off-gas performance results obtained on the DM1200 HLW Pilot Melter during processing of AZ-101 HLW simulants. The tests reported herein are a subset of six tests from a larger series of tests described in the Test Plan for the work; results from the other tests have been reported separately. The solids contents of the melter feeds were based on the WTP baseline value for the solids content of the feeds from pretreatment which changed during these tests from 20% to 15% undissolved solids resulting in tests conducted at two feed solids contents. Based on the results of earlier tests with single outlet 'J' bubblers, initial tests were performed with a total bubbling rate of 651 pm. The first set of tests (Tests 1A-1E) addressed the effects of skewing this total air flow rate back and forth between the two installed bubblers in comparison to a fixed equal division of flow between them. The second set of tests (2A-2D) addressed the effects of bubbler depth. Subsequently, as the location, type and number of bubbling outlets were varied, the optimum bubbling rate for each was determined. A third (3A-3C) and fourth (8A-8C) set of tests evaluated the effects of alternative bubbler designs with two gas outlets per bubbler instead of one by placing four bubblers in positions simulating multiple-outlet bubblers. Data from the simulated multiple outlet bubblers were used to design bubblers with two outlets for an additional set of tests (9A-9C). Test 9 was also used to determine the effect of small sugar additions to the feed on ruthenium volatility. Another set of tests (10A-10D) evaluated the effects on production rate of spiking the feed with chloride and sulfate. Variables held constant to the extent possible included melt temperature, plenum temperature, cold cap coverage, the waste simulant composition, and the target glass composition. The feed rate was increased to the point that a constant, essentially complete, cold cap was

  14. Patterns of User Engagement with Mobile- and Web-Delivered Self-Care Interventions for Adults with T2DM: A Review of the Literature.

    Science.gov (United States)

    Nelson, Lyndsay A; Coston, Taylor D; Cherrington, Andrea L; Osborn, Chandra Y

    2016-07-01

    Technology-delivered interventions can improve the health behaviors and clinical outcomes of persons with diabetes, but only if end users engage with these interventions. To summarize the current knowledge on engagement with technology-based interventions, we conducted a review of recent mobile- and web-delivered intervention studies for adults with type 2 diabetes published from 2011 to 2015. Among 163 identified studies, 24 studies satisfied our inclusion criteria. There was substantial variation in how intervention engagement was reported across studies. Engagement rates were lower among interventions with a longer duration, and engagement decreased over time. In several studies, older age and lower health literacy were associated with less engagement, and more engagement was associated with intervention improvement in at least one outcome, including glycemic control. Future technology-based intervention studies should report on engagement, examine and report on associations between user characteristics and engagement, and aim to standardize how this is reported, particularly in longer trials.

  15. Patterns of user engagement with mobile- and web-delivered self-care interventions for adults with T2DM: A review of the literature

    Science.gov (United States)

    Nelson, Lyndsay A.; Coston, Taylor D.; Cherrington, Andrea L.; Osborn, Chandra Y.

    2017-01-01

    Technology-delivered interventions can improve the health behaviors and clinical outcomes of persons with diabetes, but only if end users engage with these interventions. To summarize the current knowledge on engagement with technology-based interventions, we conducted a review of recent mobile- and web-delivered intervention studies for adults with type 2 diabetes published from 2011–2015. Among 163 identified studies, 24 studies satisfied our inclusion criteria. There was substantial variation in how intervention engagement was reported across studies. Engagement rates were lower among interventions with a longer duration, and engagement decreased over time. In several studies, older age and lower health literacy were associated with less engagement, and more engagement was associated with intervention improvement in at least one outcome, including glycemic control. Future technology-based intervention studies should report on engagement, examine and report on associations between user characteristics and engagement, and aim to standardize how this is reported, particularly in longer trials. PMID:27255269

  16. Thermally activated technologies: Technology Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2003-05-01

    The purpose of this Technology Roadmap is to outline a set of actions for government and industry to develop thermally activated technologies for converting America’s wasted heat resources into a reservoir of pollution-free energy for electric power, heating, cooling, refrigeration, and humidity control. Fuel flexibility is important. The actions also cover thermally activated technologies that use fossil fuels, biomass, and ultimately hydrogen, along with waste heat.

  17. Thermally activated technologies: Technology Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2003-05-01

    The purpose of this Technology Roadmap is to outline a set of actions for government and industry to develop thermally activated technologies for converting America’s wasted heat resources into a reservoir of pollution-free energy for electric power, heating, cooling, refrigeration, and humidity control. Fuel flexibility is important. The actions also cover thermally activated technologies that use fossil fuels, biomass, and ultimately hydrogen, along with waste heat.

  18. Plastics Technology.

    Science.gov (United States)

    Barker, Tommy G.

    This curriculum guide is designed to assist junior high schools industrial arts teachers in planning new courses and revising existing courses in plastics technology. Addressed in the individual units of the guide are the following topics: introduction to production technology; history and development of plastics; safety; youth leadership,…

  19. Lasers technology

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-01

    The Lasers Technology Program of IPEN is committed to the development of new lasers based on the research of optical materials and new technologies, as well to laser applications in several areas: Nuclear, Medicine, Dentistry, Industry, Environment and Advanced Research. The Program is basically divided into two main areas: Material and Laser Development and Laser Applications.

  20. Maritime Technology

    DEFF Research Database (Denmark)

    Sørensen, Herman

    1997-01-01

    Elementary introduction to the subject "Maritime Technology".The contents include drawings, sketches and references in English without any supplementary text.......Elementary introduction to the subject "Maritime Technology".The contents include drawings, sketches and references in English without any supplementary text....