WorldWideScience

Sample records for technology assay development

  1. Development and validation of a multiplex add-on assay of biomarkers related to sepsis using xMAP technology

    DEFF Research Database (Denmark)

    Kofoed, Kristian; Vest Schneider, Uffe; Scheel, Troels

    2006-01-01

    used markers. METHODS: We used a combination of in-house and commercially available multiplex immunoassays based on Luminex xMAP technology to assay biomarkers of potential interest in EDTA-plasma samples. RESULTS: A 3-plex assay for soluble urokinase plasminogen activator receptor (suPAR), soluble......: A commercially available xMAP panel can be expanded with markers of interest. The combined multiplex assay can measure the 8 analytes with high reproducibility. The xMAP technology is an appealing tool for assaying conventional cytokines in combination with new markers....

  2. Development of an Inhibitor Screening Assay for Mono-ADP-Ribosyl Hydrolyzing Macrodomains Using AlphaScreen Technology.

    Science.gov (United States)

    Haikarainen, Teemu; Maksimainen, Mirko M; Obaji, Ezeogo; Lehtiö, Lari

    2018-03-01

    Protein mono-ADP-ribosylation is a posttranslational modification involved in the regulation of several cellular signaling pathways. Cellular ADP-ribosylation is regulated by ADP-ribose hydrolases via a hydrolysis of the protein-linked ADP-ribose. Most of the ADP-ribose hydrolases share a macrodomain fold. Macrodomains have been linked to several diseases, such as cancer, but their cellular roles are mostly unknown. Currently, there are no inhibitors available targeting the mono-ADP-ribose hydrolyzing macrodomains. We have developed a robust AlphaScreen assay for the screening of inhibitors against macrodomains having mono-ADP-ribose hydrolysis activity. We utilized this assay for validatory screening against human MacroD1 and identified five compounds inhibiting the macrodomain. Dose-response measurements and an orthogonal assay further validated four of these compounds as MacroD1 inhibitors. The developed assay is homogenous, easy to execute, and suitable for the screening of large compound libraries. The assay principle can also be adapted for other ADP-ribose hydrolyzing macrodomains, which can utilize a biotin-mono-ADP-ribosylated protein as a substrate.

  3. Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of aflatoxin B1 in crops.

    Science.gov (United States)

    Zhao, Yong; Liu, Xiao; Wang, Xiaochen; Sun, Chongyun; Wang, Xinrui; Zhang, Pingping; Qiu, Jingfu; Yang, Ruifu; Zhou, Lei

    2016-12-01

    Contamination of grains and other crops by aflatoxin B1 (AFB1), a highly toxic aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus, poses a serious threat to human health and is an important food safety issue. In this study, a competitive up-converting phosphor technology-based lateral flow (AFB1-UPT-LF) assay was developed for rapid detection of AFB1. Detection sensitivity of the proposed assay can reach 0.03ngmL -1 for standard AFB1 solutions, with the coefficients of variation (CV) less than 10% (from 1.0 to 9.4%). A good linearity (r=0.9889) was observed for quantification of AFB1 from 0.03 to 1000ngmL -1 . Except for aflatoxin M1, no cross-reactivity was found with the abrin, ricin, ochratoxin A, botulinum toxin, shiga toxin 1, shiga toxin 2, and staphylococcal enterotoxin B, even at high concentrations of 100 or 1000ngmL - 1 . After optimizing the extraction of AFB1, the assay showed good tolerance to various crop samples, with the detection limit (from 0.1 to 5ngg - 1 ) lower than the corresponding maximum residue level (MRL) set in China. The AFB1-UPT-LF assay provides a promising tool for rapid on-site detection of AFB1 because of its high sensitivity, specificity, and sample tolerance. Copyright © 2016 Elsevier B.V. All rights reserved.

  4. Development and validation of a multiplex add-on assay of biomarkers related to sepsis using xMAP technology

    DEFF Research Database (Denmark)

    Kofoed, Kristian; Vest Schneider, Uffe; Scheel, Troels

    2006-01-01

    BACKGROUND: Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently......%, respectively. Recoveries of suPAR, sTREM-1, and MIF calibrators were 108%, 88%, and 51%, respectively. In plasma collected from 10 patients with bacterial sepsis confirmed by blood culture, the assay detected significantly increased concentrations of all 8 analytes compared with healthy controls. CONCLUSIONS...

  5. Development and validation of a multiplex add-on assay for sepsis biomarkers using xMAP technology

    DEFF Research Database (Denmark)

    Kofoed, Kristian; Schneider, Uffe Vest; Scheel, Troels

    2006-01-01

    BACKGROUND: Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently......%, respectively. Recoveries of suPAR, sTREM-1, and MIF calibrators were 108%, 88%, and 51%, respectively. In plasma collected from 10 patients with bacterial sepsis confirmed by blood culture, the assay detected significantly increased concentrations of all 8 analytes compared with healthy controls. CONCLUSIONS...

  6. Expert system technology for nondestructive waste assay

    International Nuclear Information System (INIS)

    Becker, G.K.; Determan, J.C.

    1998-01-01

    Nondestructive assay waste characterization data generated for use in the National TRU Program must be of known and demonstrable quality. Each measurement is required to receive an independent technical review by a qualified expert. An expert system prototype has been developed to automate waste NDA data review of a passive/active neutron drum counter system. The expert system is designed to yield a confidence rating regarding measurement validity. Expert system rules are derived from data in a process involving data clustering, fuzzy logic, and genetic algorithms. Expert system performance is assessed against confidence assignments elicited from waste NDA domain experts. Performance levels varied for the active, passive shielded, and passive system assay modes of the drum counter system, ranging from 78% to 94% correct classifications

  7. Expert system technology for nondestructive waste assay

    Energy Technology Data Exchange (ETDEWEB)

    Becker, G.K.; Determan, J.C.

    1998-07-01

    Nondestructive assay waste characterization data generated for use in the National TRU Program must be of known and demonstrable quality. Each measurement is required to receive an independent technical review by a qualified expert. An expert system prototype has been developed to automate waste NDA data review of a passive/active neutron drum counter system. The expert system is designed to yield a confidence rating regarding measurement validity. Expert system rules are derived from data in a process involving data clustering, fuzzy logic, and genetic algorithms. Expert system performance is assessed against confidence assignments elicited from waste NDA domain experts. Performance levels varied for the active, passive shielded, and passive system assay modes of the drum counter system, ranging from 78% to 94% correct classifications.

  8. Radioactive waste package assay facility. Volume 1. Application of assay technology

    International Nuclear Information System (INIS)

    Findlay, D.J.S.; Green, T.H.; Molesworth, T.V.; Staniforth, D.; Strachan, N.R.; Rogers, J.D.; Wise, M.O.; Forrest, K.R.

    1992-01-01

    This report, in three volumes, covers the work carried out by Taylor Woodrow Construction Ltd., and two major sub-contractors: Harwell Laboratory (AEA Technology) and Siemens Plessey Controls Ltd., on the development of a radioactive waste package assay facility, for cemented 500 litre intermediate level waste drums. In volume 1, the reasons for assay are considered together with the various techniques that can be used, and the information that can be obtained. The practical problems associated with the use of the various techniques in an integrated assay facility are identified, and the key parameters defined. Engineering and operational features are examined and provisional designs proposed for facilities at three throughput levels: 15,000, 750 and 30 drums per year respectively. The capital and operating costs for such facilities have been estimated. A number of recommendations are made for further work. 16 refs., 14 figs., 13 tabs

  9. Development of Plaque Assay Systems for Poliovirus.

    Science.gov (United States)

    1982-04-01

    inter- action must be ascertained for each type of virus to be collected and assayed. The vaccine strain of poliovirus type 1 (Sabin) was chosen as a...1067 DEVELOPMENT OF PLAQUE ASSAY SYSTEMS FOR POLIOVIRUS (U) by R.E. Fulton and K. Munroe Abstract During the summer months of 1978, Ms. Krista Munroe...quantitation of infectious poliovirus type 1. .Two different plaque assay techniques were developed and compared. The results of this work are presented

  10. The development of SRM assays is transforming proteomics research.

    Science.gov (United States)

    Manes, Nathan P; Nita-Lazar, Aleksandra

    2017-04-01

    Bottom-up targeted proteomics using SRM is a powerful analytical technology, but it requires the development of SRM assays, which is a complex procedure. Whereas proteome-wide SRM assays have recently been developed for a small number of species, this is not so for the mouse. In this issue, Percy et al. report the development of hundreds of mouse SRM assays. Their development required shotgun MS to identify proteotypic peptides, synthesis, and LC-MS characterization of peptide standards, and interlaboratory SRM to robustly assess the quality of the assays. The resulting SRM assays are intended to be used to analyze mouse plasma and cardiac tissue, primarily for cardiovascular disease and cancer research. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

  11. Elements of nondestructive assay (NDA) technology

    International Nuclear Information System (INIS)

    Hatcher, C.R.; Smith, H.

    1984-01-01

    A thorough introduction to nondestructive assay methods and instruments as they are applied to nuclear safeguards is presented. The general principles and major applications of NDA are discussed and situations in which NDA is particularly useful for nuclear safeguards purposes are described. Various passive and active γ-ray and neutron methods are examined and assay situations particularly suited to γ-ray techniques, or to neutron techniques are identified. The role of calorimetry in the NDA of plutonium-bearing materials is also discussed. The advantages and disadvantages of various NDA methods for different types of nuclear materials are mentioned

  12. Assay development status report for total cyanide

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, B.C. [Westinghouse Hanford Co., Richland, WA (United States); Jones, T.E.; Pool, K.H. [Pacific Northwest Lab., Richland, WA (United States)

    1993-02-01

    A validated cyanide assay that is applicable to a variety of tank waste matrices is necessary to resolve certain waste tank safety issues and for purposes of overall waste characterization. The target for this effort is an assay with an applicable range of greater than 1,000 ppM (0.10 wt%) total cyanide and a confidence level greater than 80%. Figure 1 illustrates the operating regime of the proposed cyanide assay method. The Assay Development Status Report for Total Cyanide will summarize the past experience with cyanide analyses on-tank waste matrices and will rate the status of the analytical methods used to assay total cyanide (CN{sup {minus}} ion) in the tank waste matrices as acceptable or unacceptable. This paper will also briefly describe the current efforts for improving analytical resolution of the assays and the attempts at speciation.

  13. Development and validation of ultraviolet spectrophotometric assay ...

    African Journals Online (AJOL)

    Liquid chromatographic methods are described in the 2005 European Pharmacopeia and 2011 British Pharmaceutical Codex for the assay of cefixime antibiotics. These official assay methods are expensive and complex for routine analysis in many pharmaceutical industries in developing countries. A simple, precise and ...

  14. Emerging Technologies and Generic Assays for the Detection of Anti-Drug Antibodies

    Directory of Open Access Journals (Sweden)

    Michael A. Partridge

    2016-01-01

    Full Text Available Anti-drug antibodies induced by biologic therapeutics often impact drug pharmacokinetics, pharmacodynamics response, clinical efficacy, and patient safety. It is critical to assess the immunogenicity risk of potential biotherapeutics in producing neutralizing and nonneutralizing anti-drug antibodies, especially in clinical phases of drug development. Different assay methodologies have been used to detect all anti-drug antibodies, including ELISA, radioimmunoassay, surface plasmon resonance, and electrochemiluminescence-based technologies. The most commonly used method is a bridging assay, performed in an ELISA or on the Meso Scale Discovery platform. In this report, we aim to review the emerging new assay technologies that can complement or address challenges associated with the bridging assay format in screening and confirmation of ADAs. We also summarize generic anti-drug antibody assays that do not require drug-specific reagents for nonclinical studies. These generic assays significantly reduce assay development efforts and, therefore, shorten the assay readiness timeline.

  15. [Development and comparative evaluation of up-converting phosphor technology based lateral flow assay for rapid detection of Yersinia pestis, Bacillus anthracis spore and Brucella spp].

    Science.gov (United States)

    Li, Chunfeng; Zhang, Pingping; Wang, Xiaoying; Liu, Xiao; Zhao, Yong; Sun, Chongyun; Wang, Chengbin; Yang, Ruifu; Zhou, Lei

    2015-01-01

    To develop an up-converting phosphor technology based lateral flow (UPT-LF) assay for rapid and quantitative detection of Yersinia pestis, Bacillus anthracis spore and Brucella spp.and make the comparison with BioThreat Alert (BTA) test strips (Tetracore Inc., USA). Using up-converting phosphor nano-particles (UCP-NPs) as the bio-marker, three double-antibody-sandwich model based UPT-LF strips including Plague-UPT-LF, Anthrax-UPT-LF, Brucella-UPT-LF were prepared and its sensitivity, accuracy, linearity and specificity were determined by detecting 10(10), 10(9), 10(8), 10(7), 10(6), 10(5) and 0 CFU/ml series of concentrations of Y.pestis, B.anthracis, Brucella standards and other 27 kinds of 10(9) CFU/ml series of contrations of bacteria strains.Furthermore, the speed, sensitivity and accuracy of bacteria standards and simulated sample detection were compared between UPT-LF and BTA system. The detection limit of Plague-UPT-LF, Anthrax-UPT-LF and Brucella-LF was 10(5) CFU/ml. The CV of series of bacteria concentrations was ≤ 15%, and the r between lg (T/C-cut-off) and lg (concentration) was 0.996,0.998 and 0.999 (F values were 1 647.57, 743.51 and 1 822.17. All the P values were Brucella-LF were excellent, while that of Anthrax-UPT-LF was a little bit regretful because of non-specific reaction with two isolates of B. subtilis and one B.cereus. On-site evaluation showed the detection time of UPT-LF for all Y.pestis, B.anthracis spore and Brucella spp.was 33, 36 and 37 min, while BTA was 115, 115 and 111 min, which revealed the higher detection speed and sensitivity of UPT-LF comparing with BTA. The negative rate of two methods for blank standard was both 5/5, the sensitivity of UPT-LF for Y.pestis,B.anthracis spore and Brucella spp. was all 10(5) CFU/ml, then BTA was 10(6), 10(6) and 10(5) CFU/ml, respectively. The detection rate of UPT-LF for all three bacteria analog positive samples was 16/16, while BTA for B.anthracis was 7/16 only. The good performance

  16. Technology Transfer of Isotopes-Based Assay: Strategies and Mechanisms

    International Nuclear Information System (INIS)

    Tabbada, R.S.D.C.; Rañada, M.L.O.; Mendoza, A.D.L.; Panganiban, R.; Castañeda, S.S.; Sombrito, E.Z.; Arcamo, S.V.R.

    2015-01-01

    Receptor Binding Assay for Paralytic Shellfish Poisoning (PSP RBA) is an isotope-based assay for detection and quantification of PSP toxins in seafood. It was established in the Philippines through a national program based on the recommendations of the Expert Mission sent by the International Atomic Energy Agency (IAEA). Through the said program, the Philippines Nuclear Research Institute (PNRI) was able to put up an RBA facility and develop expertise. Advantages of the technique against Mouse Bioassay (MBA) and high-performance Liquid Chromatography (HPLC) methods were are established. RBA is being utilized by some developed countries as screening method for Harmful Algal Bloom (HAB) Monitoring. However, it was not immediately adopted by the national HAB regulatory body for the following reasons: (1) acceptance of RBA as an official national method of analysis for PSP, (2) logistics and financial concerns in building up and maintaining a RBA facility, (3) considerations on the use of radioactive materials. To address these issues, the Philippines Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD) approved a Grants-In-Aid Project to initiate and to facilitate the transfer of the RBA technology to the monitoring and regulatory body. The project has two major objectives: capacity building and technology transfer. The capacity building focuses on human resources development of HAB monitoring personnel, specifically training on RBA and on the use of radioactive materials. On the other hand, the technology transfer deals with assistance that PNRI may render in establishing the new RBA facility and over-all know-how of the project. In this is poster, the mechanisms and strategies being undertaken by PNRI, in collaboration with the regulatory and monitoring body, to address the limitation of transferring a technology that utilizes radioactive materials including the technical difficulties are presented and discussed. (author)

  17. Development and evaluation of an up-converting phosphor technology-based lateral flow assay for the rapid, simultaneous detection of Vibrio cholerae serogroups O1 and O139.

    Science.gov (United States)

    Hao, Min; Zhang, Pingping; Li, Baisheng; Liu, Xiao; Zhao, Yong; Tan, Hailing; Sun, Chongyun; Wang, Xiaochen; Wang, Xinrui; Qiu, Haiyan; Wang, Duochun; Diao, Baowei; Jing, Huaiqi; Yang, Ruifu; Kan, Biao; Zhou, Lei

    2017-01-01

    Vibrio cholerae serogroups O1 and O139 are etiological agents of cholera, a serious and acute diarrheal disease, and rapid detection of V. cholerae is a key method for preventing and controlling cholera epidemics. Here, a point of care testing (POCT) method called Vch-UPT-LF, which is an up-converting phosphor technology-based lateral flow (UPT-LF) assay with a dual-target detection mode, was developed to detect V. cholerae O1 and O139 simultaneously from one sample loading. Although applying an independent reaction pair made both detection results for the two Vch-UPT-LF detection channels more stable, the sensitivity slightly declined from 104 to 105 colony-forming units (CFU) mL-1 compared with that of the single-target assay, while the quantification ranges covering four orders of magnitude were maintained. The strip showed excellent specificity for seven Vibrio species that are highly related genetically, and nine food-borne species whose transmission routes are similar to those of V. cholerae. The legitimate arrangement of the two adjacent test lines lessened the mutual impact of the quantitation results between the two targets, and the quantification values did not differ by more than one order of magnitude when the samples contained high concentrations of both V. cholerae O1 and O139. Under pre-incubation conditions, 1×101 CFU mL-1 of V. cholerae O1 or O139 could be detected in fewer than 7 h, while the Vch-UPT-LF assay exhibited sensitivity as high as a real-time fluorescent polymerase chain reaction with fewer false-positive results. Therefore, successful development of Vch-UPT-LF as a dual-target assay for quantitative detection makes this assay a good candidate POCT method for the detection and surveillance of epidemic cholera.

  18. Development of robotic plasma radiochemical assays for positron emission tomography

    International Nuclear Information System (INIS)

    Alexoff, D.L.; Shea, C.; Fowler, J.S.; Gatley, S.J.; Schlyer, D.J.

    1995-01-01

    A commercial laboratory robot system (Zymate PyTechnology II Laboratory Automation System; Zymark Corporation, Hopkinton, MA) was interfaced to standard and custom laboratory equipment and programmed to perform rapid radiochemical analyses for quantitative PET studies. A Zymark XP robot arm was used to carry out the determination of unchanged (parent) radiotracer in plasma using only solid phase extraction methods. Robotic throughput for the assay of parent radiotracer in plasma is 4--6 samples/hour depending on the radiotracer. Robotic assays of parent compound in plasma were validated for the radiotracers [ 11 C]Benztropine, [ 11 C]cocaine, [ 11 C]clorgyline, [ 11 C]deprenyl, [ 11 C]methadone, [ 11 C]methylphenidate, [ 11 C]raclorpride, and [ 11 C]SR46349B. A simple robot-assisted methods development strategy has been implemented to facilitate the automation of plasma assays of new radiotracers

  19. Promising new assays and technologies for the diagnosis and management of infectious diseases.

    Science.gov (United States)

    Mitsuma, S F; Mansour, M K; Dekker, J P; Kim, J; Rahman, M Z; Tweed-Kent, A; Schuetz, P

    2013-04-01

    In the first decade of the 21st century, we have seen the completion of the human genome project and marked progress in the human microbiome project. The vast amount of data generated from these efforts combined with advances in molecular and biomedical technologies have led to the development of a multitude of assays and technologies that may be useful in the diagnosis and management of infectious diseases. Here, we identify several new assays and technologies that have recently come into clinical use or have potential for clinical use in the near future. The scope of this review is broad and includes topics such as the serum marker procalcitonin, gene expression profiling, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and nucleic acid aptamers. Principles that underlie each assay or technology, their clinical applications, and potential strengths and limitations are addressed.

  20. Vitamin D assays: past and present debates, difficulties, and developments.

    Science.gov (United States)

    Fraser, William D; Milan, Anna M

    2013-02-01

    Clinical interest in Vitamin D and its purported roles not only in calcium and bone metabolism but in several other medical conditions (diabetes, cardiovascular disease, multiple sclerosis, cancer, psychiatric disorders, neuro-muscular disease) has led to a surge in laboratory requests for 25 hydroxy vitamin D and 1,25 dihydroxy vitamin D measurement. Circulating 25 hydroxy vitamin D concentration is routinely used as the best indicator of vitamin D status, but measurement of other metabolites, especially the physiologically active 1,25 dihyroxy vitamin D, are of clinical value. Over the last 40 years the development of assays for vitamin D and its metabolites from early competitive binding assays through to immunoassay and liquid chromatography aligned to mass spectrometry have demonstrated various analytical challenges, the advantages and disadvantages of each method are constantly changing with new technological developments. Immunoassay remains the predominant mode of measurement for 25-hydroxy vitamin D although problems with equimolar recovery of the D2 and D3 metabolites remain an issue. Standardisation of all assays has been improved but not resolved with the currently available reference materials as evidenced by the international vitamin D external quality assurance scheme, DEQAS. The choice of method for each laboratory remains a balance mainly between turn around time, convenience, cost and the specificity and accuracy of the information obtained. With increasing discussion and clinical interest surrounding other vitamin D metabolites the vitamin D assay debate is set to continue.

  1. Consuming technologies - developing routines

    DEFF Research Database (Denmark)

    Gram-Hanssen, Kirsten

    2008-01-01

    technologies and in this article these processes will be investigated from three different perspectives: an historical perspective of how new technologies have entered homes, a consumer perspective of how both houses and new technologies are purchased and finally, as the primary part of the article, a user...... perspective of how routines develop while these technologies are being used. In the conclusion these insights are discussed in relation to possible ways of influencing routines....

  2. Graphite technology development plan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1986-07-01

    This document presents the plan for the graphite technology development required to support the design of the 350 MW(t) Modular HTGR within the US National Gas-Cooled Reactor Program. Besides descriptions of the required technology development, cost estimates, and schedules, the plan also includes the associated design functions and design requirements.

  3. Advanced Mirror Technology Development

    Science.gov (United States)

    Stahl, H. Philip

    2017-01-01

    The Advanced Mirror Technology Development (AMTD) project matures critical technologies required to enable ultra-stable 4-m-or-larger monolithic or segmented ultraviolet, optical, and infrared (UVOIR) space telescope primary-mirror assemblies for general astrophysics and ultra-high-contrast observations of exoplanets.

  4. The Comet Assay: Tails of the (Unexpected. Use of the comet assay in pharmaceutical development.

    Directory of Open Access Journals (Sweden)

    Bas-jan Van Der Leede

    2015-08-01

    Full Text Available In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance. In this presentation we want to give insight into the circumstances in vivo comet assay is deployed in a Genetic Toxicology Department of a pharmaceutical company. As the in vivo comet assay is a salvage assay, it means that some events have occurred in an in vitro assay and that the compound (or metabolite responsible for this signal is potentially deselected for further development. More than often the decision to perform an in vivo comet assay is at a very early stage in development and the first time that the compound will be tested in vivo at high/toxic dose levels. As almost no toxicokinetic data and tissue distribution data are available a careful design with maximizes the chances for successful mitigation is necessary. Decisions on acute or repeated dosing need to be made and arrangements for combining the in vivo comet assay with the in vivo micronucleus assay are to be considered. Often synthesis methods need to be scaled up fast to provide the required amount of compound and information on suitable formulations needs to be in place. As exposure data is crucial for interpretation of results, analytical methods need to be brought in place rapidly. An experienced multi skilled and communicative team needs to be available to deploy successfully this kind of assays at an early stage of development. We will present a few scenarios on study conduct and demonstrate how this assay can make a difference for the further development of a new drug.

  5. Ocean Technology Development Tank

    Data.gov (United States)

    Federal Laboratory Consortium — The new SWFSC laboratory in La Jolla incorporates a large sea- and fresh-water Ocean Technology Development Tank. This world-class facility expands NOAA's ability to...

  6. Technology research and development

    International Nuclear Information System (INIS)

    Haas, G.M.; Abdov, M.A.; Baker, C.C.; Beuligmann, R.F.

    1985-01-01

    The U.S. Dept. of Energy discusses the new program plan, the parameters of which are a broad scientific and technology knowledge base, an attractive plasma configuration to be determined, and other issues concerning uncertainty as to what constitutes attractive fusion options to be determined in the future, and increased collaboration. Tables show changing directions in magnetic fusion energy, two examples of boundary condition impacts on long-term technology development, and priority classes of the latter. The Argonne National Laboratory comments on the relationship between science, technology and the engineering aspects of the fusion program. UCLA remarks on the role of fusion technology in the fusion program plan, particularly on results from the recent studies of FINESSE. General Dynamics offers commentary on the issues of a reduced budget, and new emphasis on science which creates an image of the program. A table illustrates technology research and development in the program plan from an industrial perspective

  7. Overcoming disease-specific matrix effect in a clinical pharmacokinetic assay using a microfluidic immunoassay technology.

    Science.gov (United States)

    Williams, Kathi; Erickson, Rich; Fischer, Saloumeh Kadkhodayan

    2017-08-01

    Etrolizumab, a humanized monoclonal antibody, has demonstrated clinical remission in a Phase II study of ulcerative colitis patients. In the Phase III program, a second indication, Crohn's disease was added. The pharmacokinetic ELISA used in the Phase I/II studies in normal human and ulcerative colitis sera exhibited matrix interference in the Crohn's disease population, necessitating implementation of a new technology. Methodology & results: Optimization of the original ELISA and assay redevelopment using different antibody pairs did not result in substantive improvements, necessitating implementation of an alternative technology for assay development. We highlight the challenges encountered with optimization/redevelopment of the original ELISA and discuss results of the new assay on the Gyros platform.

  8. Development of a loop-mediated isothermal amplification assay for ...

    African Journals Online (AJOL)

    Administrator

    2011-09-28

    Sep 28, 2011 ... A loop-mediated isothermal amplification (LAMP) assay targeting uvrC of Mycoplasma bovis was developed and evaluated. The assay specifically amplified only M. bovis; no cross-reactivity was observed for other Mycoplasma species or respiratory bacterial species. The sensitivity of the assay in.

  9. Fusion development and technology

    International Nuclear Information System (INIS)

    Montgomery, D.B.

    1991-01-01

    This report discusses the following topics: superconducting magnet technology high field superconductors; advanced magnetic system and divertor development; poloidal field coils; gyrotron development; commercial reactor studies -- Aries; ITER physics; ITER superconducting PF scenario and magnet analysis; and safety, environmental and economic factors in fusion development

  10. Developments in plutonium waste assay at AWE

    International Nuclear Information System (INIS)

    Miller, T J

    2009-01-01

    In 2002 a paper was presented at the 43rd Annual Meeting of the Institute of Nuclear Materials Management (INMM) on the assay of low level plutonium (Pu) in soft drummed waste (Miller 2002 INMM Ann. Meeting (Orlando, FL, 23-27 July 2002)). The technique described enabled the Atomic Weapons Establishment (AWE), at Aldermaston in the UK, to meet the stringent Low Level Waste Repository at Drigg (LLWRD) conditions for acceptance for the first time. However, it was initially applied to only low density waste streams because it relied on measuring the relatively low energy (60 keV) photon yield from Am-241 during growth. This paper reviews the results achieved when using the technique to assay over 10 000 waste packages and presents the case for extending the range of application to denser waste streams.

  11. Modeling error in experimental assays using the bootstrap principle: understanding discrepancies between assays using different dispensing technologies

    Science.gov (United States)

    Hanson, Sonya M.; Ekins, Sean; Chodera, John D.

    2015-12-01

    All experimental assay data contains error, but the magnitude, type, and primary origin of this error is often not obvious. Here, we describe a simple set of assay modeling techniques based on the bootstrap principle that allow sources of error and bias to be simulated and propagated into assay results. We demonstrate how deceptively simple operations—such as the creation of a dilution series with a robotic liquid handler—can significantly amplify imprecision and even contribute substantially to bias. To illustrate these techniques, we review an example of how the choice of dispensing technology can impact assay measurements, and show how large contributions to discrepancies between assays can be easily understood and potentially corrected for. These simple modeling techniques—illustrated with an accompanying IPython notebook—can allow modelers to understand the expected error and bias in experimental datasets, and even help experimentalists design assays to more effectively reach accuracy and imprecision goals.

  12. Technology transfer for development

    International Nuclear Information System (INIS)

    Abraham, D.

    1990-07-01

    The IAEA has developed a multifaceted approach to ensure that assistance to Member States results in assured technology transfer. Through advice and planning, the IAEA helps to assess the costs and benefits of a given technology, determine the basic requirements for its efficient use in conditions specific to the country, and prepare a plan for its introduction. This report describes in brief the Technical Co-operation Programmes

  13. Development of lead slowing down spectrometer for isotopic fissile assay

    International Nuclear Information System (INIS)

    Lee, Yong Deok; Park, Chang Je; Ahn, Sang Joon; Kim, Ho Dong

    2014-01-01

    A lead slowing down spectrometer (LSDS) is under development for analysis of isotopic fissile material contents in pyro-processed material, or spent fuel. Many current commercial fissile assay technologies have a limitation in accurate and direct assay of fissile content. However, LSDS is very sensitive in distinguishing fissile fission signals from each isotope. A neutron spectrum analysis was conducted in the spectrometer and the energy resolution was investigated from 0.1eV to 100keV. The spectrum was well shaped in the slowing down energy. The resolution was enough to obtain each fissile from 0.2eV to 1keV. The detector existence in the lead will disturb the source neutron spectrum. It causes a change in resolution and peak amplitude. The intense source neutron production was designed for ∼E12 n's/sec to overcome spent fuel background. The detection sensitivity of U238 and Th232 fission chamber was investigated. The first and second layer detectors increase detection efficiency. Thorium also has a threshold property to detect the fast fission neutrons from fissile fission. However, the detection of Th232 is about 76% of that of U238. A linear detection model was set up over the slowing down neutron energy to obtain each fissile material content. The isotopic fissile assay using LSDS is applicable for the optimum design of spent fuel storage to maximize burnup credit and quality assurance of the recycled nuclear material for safety and economics. LSDS technology will contribute to the transparency and credibility of pyro-process using spent fuel, as internationally demanded.

  14. Fusion development and technology

    International Nuclear Information System (INIS)

    Montgomery, D.B.

    1992-01-01

    This report discusses the following: superconducting magnet technology; high field superconductors; advanced magnetic system and divertor development; poloidal field coils; gyrotron development; commercial reactor studies--aries; ITER physics: alpha physics and alcator R ampersand D for ITER; lower hybrid current drive and heating in the ITER device; ITER superconducting PF scenario and magnet analysis; ITER systems studies; and safety, environmental and economic factors in fusion development

  15. Fusion development and technology

    Energy Technology Data Exchange (ETDEWEB)

    Montgomery, D.B.

    1992-01-01

    This report discusses the following: superconducting magnet technology; high field superconductors; advanced magnetic system and divertor development; poloidal field coils; gyrotron development; commercial reactor studies--aries; ITER physics: alpha physics and alcator R D for ITER; lower hybrid current drive and heating in the ITER device; ITER superconducting PF scenario and magnet analysis; ITER systems studies; and safety, environmental and economic factors in fusion development.

  16. Biofuel technologies. Recent developments

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Vijai Kumar [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry; MITS Univ., Rajasthan (India). Dept. of Science; Tuohy, Maria G. (eds.) [National Univ. of Ireland Galway (Ireland). Dept. of Biochemistry

    2013-02-01

    Written by experts. Richly illustrated. Of interest to both experienced researchers and beginners in the field. Biofuels are considered to be the main potential replacement for fossil fuels in the near future. In this book international experts present recent advances in biofuel research and related technologies. Topics include biomethane and biobutanol production, microbial fuel cells, feedstock production, biomass pre-treatment, enzyme hydrolysis, genetic manipulation of microbial cells and their application in the biofuels industry, bioreactor systems, and economical processing technologies for biofuel residues. The chapters provide concise information to help understand the technology-related implications of biofuels development. Moreover, recent updates on biofuel feedstocks, biofuel types, associated co- and byproducts and their applications are highlighted. The book addresses the needs of postgraduate researchers and scientists across diverse disciplines and industrial sectors in which biofuel technologies and related research and experimentation are pursued.

  17. Enzyme assays.

    Science.gov (United States)

    Brodelius, P E

    1991-02-01

    The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay systems.

  18. Developing a yeast-based assay protocol to monitor total ...

    African Journals Online (AJOL)

    A yeast-based assay protocol developed for detecting oestrogenic activity in activated sludge (AS) supernatant is described. The protocol used Saccharomyces cerevisiae construct RMY/ER-ERE with human oestrogen receptor (ERα) and lacZ reporter genes, and was developed by modifying existing assays for use with AS ...

  19. Development of a TaqMan assay for the six major genotypes of hepatitis C virus: Comparison with commercial assays

    DEFF Research Database (Denmark)

    Engle, Ronald E; Russell, Rodney S; Purcell, Robert H

    2008-01-01

    A quantitative real-time PCR assay was developed that detects genomic RNA from reference strains representing the six major genotypes of hepatitis C virus (HCV) with equal sensitivity and accurately measured HCV RNA in JFH1 HCV-infected Huh7.5 cells. The method is indirectly calibrated to the first...... international (WHO 96/790) HCV standard preparation and has a linear dynamic range of 10(2.6)-10(6.5) IU/ml. In addition, the inter- and intra-assay precision were approximately 3% CV and HCV RNA Nucleic Acid Technology kits...... (Versant HCV RNA 3.0 b-DNA and Amplicor HCV Monitor), that also employ the WHO standard, allowed validation of the TaqMan assay against all major HCV genotypes. Both commercial methods detected HCV RNA over a wide dynamic range, but showed a consistent difference of about 0.3 log10 when evaluating samples...

  20. ABC Technology Development Program

    International Nuclear Information System (INIS)

    1994-01-01

    The Accelerator-Based Conversion (ABC) facility will be designed to accomplish the following mission: 'Provide a weapon's grade plutonium disposition capability in a safe, economical, and environmentally sound manner on a prudent schedule for [50] tons of weapon's grade plutonium to be disposed on in [20] years.' This mission is supported by four major objectives: provide a reliable plutonium disposition capability within the next [15] years; provide a level of safety and of safety assurance that meets or exceeds that afforded to the public by modern commercial nuclear power plants; meet or exceed all applicable federal, state, and local regulations or standards for environmental compliance; manage the program in a cost effective manner. The ABC Technology Development Program defines the technology development activities that are required to accomplish this mission. The technology development tasks are related to the following topics: blanket system; vessel systems; reactivity control systems; heat transport system components; energy conversion systems; shutdown heat transport systems components; auxiliary systems; technology demonstrations - large scale experiments

  1. Development of homogeneous luminescence assays for histone demethylase catalysis and binding.

    Science.gov (United States)

    Kawamura, Akane; Tumber, Anthony; Rose, Nathan R; King, Oliver N F; Daniel, Michelle; Oppermann, Udo; Heightman, Tom D; Schofield, Christopher

    2010-09-01

    Covalent modifications to histones play important roles in chromatin dynamics and the regulation of gene expression. The JumonjiC (JmjC)-containing histone demethylases (HDMs) catalyze the demethylation of methylated lysine residues on histone tails. Here we report the development of homogeneous luminescence-based assay methods for measuring the catalytic activity and the binding affinities of peptides to HDMs. The assays use amplified luminescent proximity homogeneous assay (ALPHA) technology, are sensitive and robust, and can be used for small molecule inhibitor screening of HDMs. We have profiled known inhibitors of JMJD2E and demonstrate a correlation between the inhibitor potencies determined by the ALPHA and other types of assays. Although this study focuses on the JMJD2E isoform, the catalytic turnover and binding assays described here can be used in studies on other HDMs. The assays should be useful for the development of small molecule inhibitors selective for HDM isoforms. 2010 Elsevier Inc. All rights reserved.

  2. Robotics Technology Development Program

    International Nuclear Information System (INIS)

    1994-02-01

    The Robotics Technology Development Program (RTDP) is a ''needs-driven'' effort. A lengthy series of presentations and discussions at DOE sites considered critical to DOE's Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination ampersand Dismantlement (D ampersand D). The RTDP Group realized that much of the technology development was common (Cross Cutting-CC) to each of these robotics application areas, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT ampersand E) process urged an additional organizational break-out between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). The RDTP is thus organized around these application areas -- TWR, CAA, MWO, D ampersand D and CC ampersand AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas

  3. Transmutation Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Song, T. Y.; Park, W. S.; Kim, Y. H. (and others)

    2007-06-15

    The spent fuel coming from the PWR is one of the most difficult problems to be solved for the continuous use of nuclear power. It takes a few million years to be safe under the ground. Therefore, it is not easy to take care of the spent fuel for such a long time. Transmutation technology is the key technology which can solve the spent fuel problem basically. Transmutation is to transmute long-lived radioactive nuclides in the spent fuel into short-lived or stable nuclide through nuclear reactions. The long-lived radioactive nuclides can be TRU and fission products such as Tc-99 and I-129. Although the transmutation technology does not make the underground disposal totally unnecessary, the period to take care of the spent fuel can be reduced to the order of a few hundred years. In addition to the environmental benefit, transmutation can be considered to recycle the energy in the spent fuel since the transmutation is performed through nuclear fission reaction of the TRU in the spent fuel. Therefore, transmutation technology is worth being developed in economical aspect. The results of this work can be a basis for the next stage research. The objective of the third stage research was to complete the core conceptual design and verification of the key technologies. The final results will contribute to the establishment of Korean back end fuel cycle policy by providing technical guidelines.

  4. ECH Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Temkin, Richard [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2014-12-24

    Electron Cyclotron Heating (ECH) is needed for plasma heating, current drive, plasma stability control, and other applications in fusion energy sciences research. The program of fusion energy sciences supported by U. S. DOE, Office of Science, Fusion Energy Sciences relies on the development of ECH technology to meet the needs of several plasma devices working at the frontier of fusion energy sciences research. The largest operating ECH system in the world is at DIII-D, consisting of six 1 MW, 110 GHz gyrotrons capable of ten second pulsed operation, plus two newer gyrotrons. The ECH Technology Development research program investigated the options for upgrading the DIII-D 110 GHz ECH system. Options included extending present-day 1 MW technology to 1.3 – 1.5 MW power levels or developing an entirely new approach to achieve up to 2 MW of power per gyrotron. The research consisted of theoretical research and designs conducted by Communication and Power Industries of Palo Alto, CA working with MIT. Results of the study would be validated in a later phase by research on short pulse length gyrotrons at MIT and long pulse / cw gyrotrons in industry. This research follows a highly successful program of development that has led to the highly reliable, six megawatt ECH system at the DIII-D tokamak. Eventually, gyrotrons at the 1.5 megawatt to multi-megawatt power level will be needed for heating and current drive in large scale plasmas including ITER and DEMO.

  5. Graphite Technology Development Plan

    Energy Technology Data Exchange (ETDEWEB)

    W. Windes; T. Burchell; R. Bratton

    2007-09-01

    This technology development plan is designed to provide a clear understanding of the research and development direction necessary for the qualification of nuclear grade graphite for use within the Next Generation Nuclear Plant (NGNP) reactor. The NGNP will be a helium gas cooled Very High Temperature Reactor (VHTR) with a large graphite core. Graphite physically contains the fuel and comprises the majority of the core volume. Considerable effort will be required to ensure that the graphite performance is not compromised during operation. Based upon the perceived requirements the major data needs are outlined and justified from the perspective of reactor design, reatcor performance, or the reactor safety case. The path forward for technology development can then be easily determined for each data need. How the data will be obtained and the inter-relationships between the experimental and modeling activities will define the technology development for graphite R&D. Finally, the variables affecting this R&D program are discussed from a general perspective. Factors that can significantly affect the R&D program such as funding, schedules, available resources, multiple reactor designs, and graphite acquisition are analyzed.

  6. Graphite Technology Development Plan

    Energy Technology Data Exchange (ETDEWEB)

    W. Windes; T. Burchell; M.Carroll

    2010-10-01

    The Next Generation Nuclear Plant (NGNP) will be a helium-cooled High Temperature Gas Reactor (HTGR) with a large graphite core. Graphite physically contains the fuel and comprises the majority of the core volume. Graphite has been used effectively as a structural and moderator material in both research and commercial high-temperature gas-cooled reactors. This development has resulted in graphite being established as a viable structural material for HTGRs. While the general characteristics necessary for producing nuclear grade graphite are understood, historical “nuclear” grades no longer exist. New grades must be fabricated, characterized, and irradiated to demonstrate that current grades of graphite exhibit acceptable non-irradiated and irradiated properties upon which the thermomechanical design of the structural graphite in NGNP is based. This Technology Development Plan outlines the research and development (R&D) activities and associated rationale necessary to qualify nuclear grade graphite for use within the NGNP reactor.

  7. Developing technologies and resources

    International Nuclear Information System (INIS)

    Walker, R.S.

    2015-01-01

    Our success as a nuclear nation rests on interdependent pillars involving industry, governments, regulators, and academia. In a context of coherent public policy, we must achieve: 5 Nuclear Industry Priorities: Ensure refurbishments are completed to cost and schedule; Achieve Canadian supply chain success in international nuclear business; Support a strong Canadian nuclear science, technology and innovation agenda; Enhance the supply of skilled workers; Develop a coordinated and integrated strategy for the long term management of all radioactive waste materials; Refine communication strategies informed by insights from social sciences. Canada's nuclear sector has the opportunity to adapt to the opportunities presented by having a national laboratory in Canada.

  8. Telemetric Technologies for the Assay of Gene Expression

    Science.gov (United States)

    Paul, Anna-Lisa; Bamsey, Matthew; Berinstain, Alain; Neron, Philip; Graham, Thomas; Ferl, Robert

    Telemetric data collection has been widely used in spaceflight applications where human participation is limited (orbital mission payloads) or unfeasible (planetary landers, satellites, and probes). The transmission of digital data from electronic sensors of typical environmental parameters, growth patterns and physical properties of materials is routine telemetry, and even the collection and transmission of deep space images is a standard tool of astrophysics. But telemetric imaging for current biological payloads has thus far been limited to the collection of standard white-light photography that is largely confined to reporting the surface characteristics of the specimens involved. Advances in imaging technologies that facilitate the collection of a variety of light wavelengths will expand the science return on biological payloads to include evaluations of the molecular genetic response of organisms to the spaceflight or extraterrestrial environment, with minimal or no human intervention. Advanced imaging technology in combination with biologically engineered sensor organisms can create a system that can report via telemetry on the patterns of gene expression required to adapt to a novel environment. The utilization of genetically engineered plants as biosensors has made elegant strides in the recent years, providing keen insights into the health of plants in general and particularly in the nature and cellular location of stress responses. Moreover, molecular responses to gravitational vectors have been elegantly analyzed with fluorescent tools. Green Fluorescence Protein (GFP) and other fluorophores have made it possible for analyses of gene expression and biological responses to occur telemetrically, with the information potentially delivered to the investigator over large distances as simple, preprocessed fluorescence images. Having previously deployed transgenic plant biosensors to evaluate responses to orbital spaceflight, we wish to develop both the plants

  9. Nondestructive assay technology and in-plant dynamic materials control: ''DYMAC''

    International Nuclear Information System (INIS)

    Keppin, G.R.; Maraman, W.J.

    1975-01-01

    An advanced system of in-plant materials control known as DYMAC, Dynamic Materials Control, is being developed. This major safeguards R and D effort merges state-of-the-art nondestructive assay instrumentation and computer technology, with the clear objective of demonstrating a workable, cost-effective system of stringent, real time control of nuclear materials in a modern plutonium processing facility. Emphasis is placed on developing practical solutions to generic problems of materials measurement and control, so that resulting safeguards techniques and instrumentation will have widespread applicability throughout the nuclear community. (auth)

  10. Development of sodium technology

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Sung Tai; Nam, H. Y.; Choi, Y. D. [and others

    2000-05-01

    The objective of present study is to produce the experimental data for development and verification of computer codes for development of LMR and to develop the preliminary technologies for the future large scale verification experiments. A MHD experimental test loop has been constructed for the quantitative analysis of the effect of magnetic field on the sodium flow and experiments are carried out for three EM pumps. The previous pressure drop correlations are evaluated using the experimental data obtained from the pressure drop experiment in a 19-pin fuel assembly with wire spacer. An dimensionless variable is proposed to describe the amplitude and frequency of the fluctuation of free surface using the experimental data obtained from free surface experimental apparatus and an empirical correlation is developed using this dimensionless variable. An experimental test loop is constructed to measure the flow characteristics in IHX shell side and the local pressure drop in fuel assembly, and to test the vibration behaviour of fuel pins due to flow induced vibration. The sodium two-phase flow measuring technique using the electromagnetic flowmeter is developed and the sodium differential pressure drop measuring technique using the method of direct contact of sodium and oil is established. The work on the analysis of sodium fire characteristics and produce data for vlidation of computer code is performed. Perfect reopen time of self plugged leak path was observed to be about 130 minutes after water leak initiation. Reopen shape of a specimen appeared to be double layer of circular type, and reopen size of this specimen surface was about 2mm diameter on sodium side. In small water leakage experiments, the following correlation equation about the reopen time between sodium temperature and initial leak rate was obtained, {tau}{sub c} = {delta}{center_dot}g{sup -0.83}{center_dot}10{sup (3570/T{sub Na}-3.34)}, in 400-500 deg C of liquid sodium atmosphere. The characteristics

  11. Development of sodium technology

    International Nuclear Information System (INIS)

    Hwang, Sung Tai; Nam, H. Y.; Choi, Y. D.

    2000-05-01

    The objective of present study is to produce the experimental data for development and verification of computer codes for development of LMR and to develop the preliminary technologies for the future large scale verification experiments. A MHD experimental test loop has been constructed for the quantitative analysis of the effect of magnetic field on the sodium flow and experiments are carried out for three EM pumps. The previous pressure drop correlations are evaluated using the experimental data obtained from the pressure drop experiment in a 19-pin fuel assembly with wire spacer. An dimensionless variable is proposed to describe the amplitude and frequency of the fluctuation of free surface using the experimental data obtained from free surface experimental apparatus and an empirical correlation is developed using this dimensionless variable. An experimental test loop is constructed to measure the flow characteristics in IHX shell side and the local pressure drop in fuel assembly, and to test the vibration behaviour of fuel pins due to flow induced vibration. The sodium two-phase flow measuring technique using the electromagnetic flowmeter is developed and the sodium differential pressure drop measuring technique using the method of direct contact of sodium and oil is established. The work on the analysis of sodium fire characteristics and produce data for vlidation of computer code is performed. Perfect reopen time of self plugged leak path was observed to be about 130 minutes after water leak initiation. Reopen shape of a specimen appeared to be double layer of circular type, and reopen size of this specimen surface was about 2mm diameter on sodium side. In small water leakage experiments, the following correlation equation about the reopen time between sodium temperature and initial leak rate was obtained, τ c = δ·g -0.83 ·10 (3570/T Na -3.34) , in 400-500 deg C of liquid sodium atmosphere. The characteristics of pressure propagation and gas flow, and

  12. Technology Development Facility (TDF)

    International Nuclear Information System (INIS)

    Doggett, J.N.

    1982-01-01

    We have been studying small, driven, magnetic-mirror-based fusion reactors for the Technology Development Facility (TDF), that will test fusion reactor materials, components, and subsystems. Magnetic mirror systems are particularly interesting for this application because of their inherent steady-state operation, potentially high neutron wall loading, and relatively small size. Our design is a tandem mirror device first described by Fowler and Logan, based on the physics of the TMX experiments at Lawrence Livermore National Laboratory (LLNL). The device produces 20 MW of fusion power with a first-wall, uncollided 14-MeV neutron flux of 1.4 MW/m 2 on an area of approximately 8 m 2 , while consuming approximately 250 MW of electrical power. The work was done by a combined industrial-laboratory-university group

  13. Development of Korea telecommunication technology

    International Nuclear Information System (INIS)

    1992-06-01

    It concentrates on development of Korea telecommunication technology, which is made up seven chapters. It gives description of manual central telephone exchange or private automatic telephone exchange, transmission technology on wire line and cable line technology and optical transmission, radio communication technology on mobile and natural satellite communication, network technology with intelligent network, broadband ISDN and packet switched Data Network, terminal technology with telephone and data communication terminal and development of Information Technology in Korea. It has an appendix about development of military communication system.

  14. Technology Development of Safeguards

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ho Dong; Kang, H. Y.; Ko, W. I. (and others)

    2007-04-15

    The objective of this project is to perform R and D on the essential technologies in nuclear material measurement and surveillance and verification system, and to improve the state of being transparent on the nuclear material management of DUPIC Fuel Development Facility (DFDF) through the evaluation of safeguard ability on non-proliferation fuel cycle and nuclear proliferation resistance. Nuclear material position scan system for the reduction of measurement error was developed for the spatial distribution search of spent fuel in DUPIC facility. Web-based realtime remote monitoring system was designed and constructed for satisfying the IAEA's performance criteria of continuous monitoring, and also developed a software for the function of remote control and message. And diversion paths in a proliferation resistant pyroprocess for SFR were analyzed and its protecting system against the diversion paths were suggested for enhancing proliferation resistance of advanced nuclear fuel cycle. These results could be used for planning the further R and D items in the area of safeguards. Those R and D results mentioned above would be helpful for increasing Korean nuclear transparency in the future.

  15. Developments in lubricant technology

    CERN Document Server

    Srivastava, S P

    2014-01-01

    Provides a fundamental understanding of lubricants and lubricant technology including emerging lubricants such as synthetic and environmentally friendly lubricants Teaches the reader to understand the role of technology involved in the manufacture of lubricants Details both major industrial oils and automotive oils for various engines Covers emerging lubricant technology such as synthetic and environmentally friendly lubricants Discusses lubricant blending technology, storage, re-refining and condition monitoring of lubricant in equipment

  16. New Technology For Fissile Assay In Spent Fuel Using LSDS

    International Nuclear Information System (INIS)

    Lee, Yongdeok; Park, Changje; Park, Geunil; Lee, Jungwon; Song, Keechan

    2012-01-01

    The principle of LSDS is very simple. The interrogated neutron induces energy dependent characteristic fission from fissile materials in spent fuel. The fission threshold detector screens the prompt fast fission neutrons from background and fissionable materials. However, intense source neutron is necessary to overcome radiation background. The detected signals have a direct relationship to the content of each fissile material. The isotopic fissile assay using LSDS is applicable for optimum design of spent fuel storage and management, quality assurance of recycled nuclear material, maximization of burnup credit. Another important application is verity burnup code and provide correction factor for improving the fissile material content, fission product correction factor for improving the fissile material content, fission product content and theoretical burnup. Additionally, the isotopic fissile content assay will increase the transparence and credibility for spent fuel storage and its re-utilization, as internationally demanded

  17. Status of LSDS Development for Isotopic Fissile Assay in Used Fuel

    International Nuclear Information System (INIS)

    Lee, Y.D.; Ahn, S.; Kim, H.-D.; Song, K.C.; Park, C.J.

    2015-01-01

    Because of the large amount accumulation of spent fuel, a research to solve the spent fuel problem is actively performed in Korea. One option is to develop the SFR linked with the pyro process to reuse the existing fissile materials in spent fuel. Therefore, an accurate isotopic fissile content assay becomes a key factor in the reuse of fissile material for safety and safeguards purpose. There are several commercial non-destructive technologies for nuclear material assay. However, technology for direct isotopic fissile content assay in spent fuel is not developed yet. Internationally, a verification of special nuclear material in spent fuel, mainly U-235, Pu239, Pu241, is very important for the safeguards objective. These fissile materials can be misused for nuclear weapon purpose, not for peaceful use. As a future nuclear system is developed,, improved safeguards technology must be developed for an approval of fissile materials. A direct measurement of fissile materials is very important to provide a continuous of knowledge on nuclear materials. LSDS (Lead Slowing Down Spectrometer) has an advantage to assay an isotopic fissile content directly, without any help of burnup code and history. LSDS system is under development in KAERI (Korea Atomic Energy Research Institute) for spent fuel and recycled fuel. A linear assay model was setup for U235, Pu239 and Pu241. The dominant individual fission characteristic is appeared between 0.1 eV and 1 keV range. An electron linear accelerator for compact and low cost is under development to produce high source neutron effectively and efficiently. The LSDS is also applicable for optimum design of spent fuel storage and management. The advanced fissile assay technology will contribute to increase the transparency and credibility internationally on a reuse of fissile materials in future nuclear energy system development. (author)

  18. Fluidic Force Discrimination Assays: A New Technology for Tetrodotoxin Detection

    Directory of Open Access Journals (Sweden)

    Cy R. Tamanaha

    2010-03-01

    Full Text Available Tetrodotoxin (TTX is a low molecular weight (~319 Da neurotoxin found in a number of animal species, including pufferfish. Protection from toxin tainted food stuffs requires rapid, sensitive, and specific diagnostic tests. An emerging technique for the detection of both proteins and nucleic acids is Fluidic Force Discrimination (FFD assays. This simple and rapid method typically uses a sandwich immunoassay format labeled with micrometer-diameter beads and has the novel capability of removing nonspecifically attached beads under controlled, fluidic conditions. This technique allows for near real-time, multiplexed analysis at levels of detection that exceed many of the conventional transduction methods (e.g., ELISAs. In addition, the large linear dynamic range afforded by FFD should decrease the need to perform multiple sample dilutions, a common challenge for food testing. By applying FFD assays to an inhibition immunoassay platform specific for TTX and transduction via low magnification microscopy, levels of detection of ~15 ng/mL and linear dynamic ranges of 4 to 5 orders of magnitude were achieved. The results from these studies on the first small molecule FFD assay, along with the impact to detection of seafood toxins, will be discussed in this manuscript.

  19. MELTED BUTTER TECHNOLOGY DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    L. V. Golubeva

    2014-01-01

    Full Text Available Summary. Melted butter is made from dairy butter by rendering the fat phase. It has specific taste and aroma, high-calorie content and good assimilability. Defects of butter which appeared during the storage causes by the development of microbiological processes or by the chemical oxidation. On the development of these processes influence quality and composition of fresh butter, its physical structure, content of the increased amount of gas phase and content of heavy metals, storage conditions. Microbiological spoilage of butter occurs generally due to damage of plasma which is good environment for the development of microorganisms. Defects of microbiological origin include: unclean, sour, moldy, yeasty, cheesy, bitter taste. Defects of test and smell chemical origin are formed due to hydrolytic digestion of lipids. It's prevailed at long storage of butter in the conditions of freezing temperatures. It's picked out the following main processes of spoiling: souring, acidifying and sallowness. Often these processes take place simultaneously.It has been investigated melted butter with lactated additive. The latter improves the microbiological and toxicological safety, prolongs the storage condition of the products. Technological efficiency of the additives is achieved by a multilayer products formation from the inactive bound water, preventing microorganisms growth and by the barrier layer with lactate inhibiting hydrolytic reactions. Oil samples were obtained with the batch-type butter maker application, then they were melted and after that lactated additive were supplemented. It has been studied organoleptic and physico-chemical indices of the melted butter samples. The fatty-acid composition of melted butter were studied. Comparative analysis of fatty-acid composition of cow's milk fat and produced melted butter has shown their similarity. Also in the last sample there is increased weight fraction of linoleic and linolenic acids. The obtained

  20. Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue.

    Science.gov (United States)

    Miles, Timothy D; Martin, Frank N; Coffey, Michael D

    2015-02-01

    Several isothermal amplification techniques recently have been developed that are tolerant of inhibitors present in many plant extracts, which can reduce the need for obtaining purified DNA for running diagnostic assays. One such commercially available technique that has similarities with real-time polymerase chain reaction (PCR) for designing primers and a labeled probe is recombinase polymerase amplification (RPA). This technology was used to develop two simple and rapid approaches for detection of Phytophthora spp.: one genus-specific assay multiplexed with a plant internal control and the other species-specific assays for Phytophthora ramorum and P. kernoviae. All assays were tested for sensitivity (ranging from 3 ng to 1 fg of DNA) and specificity using DNA extracted from more than 136 Phytophthora taxa, 21 Pythium spp., 1 Phytopythium sp., and a wide range of plant species. The lower limit of linear detection using purified DNA was 200 to 300 fg of DNA in all pathogen RPA assays. Six different extraction buffers were tested for use during plant tissue maceration and the assays were validated in the field by collecting 222 symptomatic plant samples from over 50 different hosts. Only 56 samples were culture positive for Phytophthora spp. whereas 91 were positive using the Phytophthora genus-specific RPA test and a TaqMan real-time PCR assay. A technique for the generation of sequencing templates from positive RPA amplifications to confirm species identification was also developed. These RPA assays have added benefits over traditional technologies because they are rapid (results can be obtained in as little as 15 min), do not require DNA extraction or extensive training to complete, use less expensive portable equipment than PCR-based assays, and are significantly more specific than current immunologically based methods. This should provide a rapid, field-deployable capability for pathogen detection that will facilitate point-of-sample collection processing

  1. Assay Development Process | Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    Typical steps involved in the development of a  mass spectrometry-based targeted assay include: (1) selection of surrogate or signature peptides corresponding to the targeted protein or modification of interest; (2) iterative optimization of instrument and method parameters for optimal detection of the selected peptide; (3) method development for protein extraction from biological matrices such as tissue, whole cell lysates, or blood plasma/serum and proteolytic digestion of proteins (usually with trypsin); (4) evaluation of the assay in the intended biological matrix to determine if e

  2. Smart Fabrics Technology Development

    Science.gov (United States)

    Simon, Cory; Potter, Elliott; Potter, Elliott; McCabe, Mary; Baggerman, Clint

    2010-01-01

    Advances in Smart Fabrics technology are enabling an exciting array of new applications for NASA exploration missions, the biomedical community, and consumer electronics. This report summarizes the findings of a brief investigation into the state of the art and potential applications of smart fabrics to address challenges in human spaceflight.

  3. The development of information technologies

    OpenAIRE

    Kostúr Karol

    2002-01-01

    The contribution analyses the tasks information technologies in industry. At present time is the problem of connecting mutual variously levels of management. Therefore, first priority is an integration of information technologies. The information technologies for enterprise management are analysed. The product SAP R/3 appears as suitable for top management. The SAP R/3 Enterprise has a new technology so call web-server. This server enables the integration with e-business. The development my S...

  4. Working Environment and Technological Development

    DEFF Research Database (Denmark)

    Clausen, Christian; Nielsen, Klaus T.; Jensen, Per Langaa

    1997-01-01

    The paper describes the purpose, themes, overarching research questions and specific projects of the programme: Working Environment and Technological Development. The major research themes are:1) Management concepts and the working environment, which considers the visions...... and their and their concept of working environment2) Technology renewal, which considers the role of the working environment in connection with the development and use of concrete technologies3) Working environment planning, which considers the existing efforts to place the working environment in a planning process....

  5. Development of an enzyme-linked immunosorbent assay for toosendanin.

    Science.gov (United States)

    Zhang, Jing; Feng, Gang; Luo, Li; Yu, Xiang Yang; Ma, Zhi Qing; Feng, Jun Tao; Liu, Xian Jin; Zhang, Xing

    2008-08-01

    Enzyme-linked immunosorbent assays (ELISAs) were developed by using polyclonal antibody for toosendanin (TSN), a biopesticide from Melai toosendan Sieb. et Zucc. Their application in the determination of this analyte in spiked cabbage, tomato and apple samples was studied. The haptens, 28-hemisuccinyl-TSN (TSN-S) and 28-hemiglutaryl-TSN (TSN-G) were synthesized by using esterification. Immunogen and coating antigen were synthesized by using the mixed anhydride reaction and active ester protocol, respectively. Rabbits were immunized with TSN-G-BSA and TSN-S-BSA. Using the selected antibody and coating antigen, an indirect competitive ELISA for TSN was developed, which showed an IC(50) value of 1.023 microg mL(-1), with a detection limit of 0.009 microg mL(-1). A direct competitive ELISA using an enzyme tracer was also developed. The assay showed an IC(50) value of 0.840 microg mL(-1) with a detection limit of 0.014 microg mL(-1). Both assays displayed high cross-reactivity to a closely structurally related compound. Recoveries of TSN from both immunoassays of fortified samples ranged from 76.4% to 113.2% and 75.1% to 132.3%, respectively. Linear regression analysis showed good correlation between the TSN concentrations derived from ELISA and HPLC analyses, which suggested that the ELISA is a convenient supplementary analytical tool for monitoring TSN.

  6. Economic and technical assay of the application of radionuclide technology

    International Nuclear Information System (INIS)

    Emrich, F.G.

    1976-05-01

    The dynamically loaded components of combustion engines undergo wear during operation as a function of the operating points and the operating parameters selected. Radionuclide measurement methods developed in the Federal Republic of Germany in the LIT laboratory of Gesellschaft fuer Kernforschung allow to determine the wear of machine components during operation, contrary to conventional length measurement techniques. In the first part of this work the existing wear problems will be analyzed and investigations will be made into the capacities of conventional and radionuclide measurement methods. Based on this, a cost-benefit analysis system was developed for evaluation under technical and economical aspects of the use of different measurement methods allowing to solve detailed wear problems. This system furnishes results on the respective optimum wear measurement method relating to the actual problem and taking into account the relevant technical and economical boundary conditions. (orig.) [de

  7. Development of a multiplexed urine assay for prostate cancer diagnosis.

    Science.gov (United States)

    Vener, Tatiana; Derecho, Carlo; Baden, Jonathan; Wang, Haiying; Rajpurohit, Yashoda; Skelton, Joanne; Mehrotra, Jyoti; Varde, Shobha; Chowdary, Dondapati; Stallings, Walt; Leibovich, Bradley; Robin, Howard; Pelzer, Alexandre; Schäfer, Georg; Auprich, Marco; Mannweiler, Sebastian; Amersdorfer, Peter; Mazumder, Abhijit

    2008-05-01

    Several studies have demonstrated the value of DNA methylation in urine-based assays for prostate cancer diagnosis. However, a multicenter validation with a clinical prototype has not been published. We developed a multiplexed, quantitative methylation-specific polymerase chain reaction (MSP) assay consisting of 3 methylation markers, GSTP1, RARB, and APC, and an endogenous control, ACTB, in a closed-tube, homogeneous assay format. We tested this format with urine samples collected after digital rectal examination from 234 patients with prostate-specific antigen (PSA) concentrations > or =2.5 microg/L in 2 independent patient cohorts from 9 clinical sites. In the first cohort of 121 patients, we demonstrated 55% sensitivity and 80% specificity, with area under the curve (AUC) 0.69. In the second independent cohort of 113 patients, we found a comparable sensitivity of 53% and specificity of 76% (AUC 0.65). In the first cohort, as well as in a combined cohort, the MSP assay in conjunction with total PSA, digital rectal examination status, and age improved the AUC without MSP, although the difference was not statistically significant. Importantly, the GSTP1 cycle threshold value demonstrated a good correlation (R = 0.84) with the number of cores found to contain prostate cancer or premalignant lesions on biopsy. Moreover, samples that exhibited methylation for either GSTP1 or RARB typically contained higher tumor volumes at prostatectomy than those samples that did not exhibit methylation. These data confirm and extend previously reported studies and demonstrate the performance of a clinical prototype assay that should aid urologists in identifying men who should undergo biopsy.

  8. Development of in vitro assay method with radioisotope

    International Nuclear Information System (INIS)

    Choi, Chang Woon; Lim, S. M.; An, S. H.; Woo, K. S.; Chung, W. S.; Lim, S. J.; Hong, S. W.; Oh, O. D.

    1999-04-01

    Radioimmunoassay (RIA) and related competitive protein-binding methods began a little over 20 years ago as a cumbersome research methodology in a few specialized laboratories. Endocrinology has been greatly enriched by the new knowledge that has come as a direct result of RIA methods. Establishment of the taxol RIA system will be expected to develop RIA for drug monitoring. Scintillation proximity assay was useful since any separation step is not required, it has the advantage of dealing with multiple samples. The increased sensitivity of the new assay in determining HCV RT([ 125 I]dUTP) suggests that it would be worth investigating whether the system can be applied to analysis. [ 125 I] lodotyramine with 98.5% radiochemical purity. Optimal background counts was certificated using varied radioactivity of radionuclides. Appropriate standard curve was obtained from SPA method successively, and the concentration of hCG from unknown serum was determined by standard curve. The result concentration of hCG from unknown serum was determined by synthesized successively and purified by HPLC system. Hybridoma reducing monoclonal anti thyroglobulin antibodies titer is measured by ELISA. These studies play an important role in development of in vitro assay with radionuclides

  9. Development of in vitro assay method with radioisotope

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Chang Woon; Lim, S. M.; An, S. H.; Woo, K. S.; Chung, W. S.; Lim, S. J.; Hong, S. W. [Korea Atomic Energy Research Institute. Korea Cancer Center Hospital, Seoul (Korea, Republic of); Oh, O. D. [Yonsei University, Seoul (Korea, Republic of)

    1999-04-01

    Radioimmunoassay (RIA) and related competitive protein-binding methods began a little over 20 years ago as a cumbersome research methodology in a few specialized laboratories. Endocrinology has been greatly enriched by the new knowledge that has come as a direct result of RIA methods. Establishment of the taxol RIA system will be expected to develop RIA for drug monitoring. Scintillation proximity assay was useful since any separation step is not required, it has the advantage of dealing with multiple samples. The increased sensitivity of the new assay in determining HCV RT([{sup 125}I]dUTP) suggests that it would be worth investigating whether the system can be applied to analysis. [{sup 125}I] lodotyramine with 98.5% radiochemical purity. Optimal background counts was certificated using varied radioactivity of radionuclides. Appropriate standard curve was obtained from SPA method successively, and the concentration of hCG from unknown serum was determined by standard curve. The result concentration of hCG from unknown serum was determined by synthesized successively and purified by HPLC system. Hybridoma reducing monoclonal anti thyroglobulin antibodies titer is measured by ELISA. These studies play an important role in development of in vitro assay with radionuclides.

  10. Development of a lion-specific interferon-gamma assay.

    Science.gov (United States)

    Maas, M; van Kooten, P J S; Schreuder, J; Morar, D; Tijhaar, E; Michel, A L; Rutten, V P M G

    2012-10-15

    The ongoing spread of bovine tuberculosis (BTB) in African free-ranging lion populations, for example in the Kruger National Park, raises the need for diagnostic assays for BTB in lions. These, in addition, would be highly relevant for zoological gardens worldwide that want to determine the BTB status of their lions, e.g. for translocations. The present study concerns the development of a lion-specific IFN-γ assay, following the production and characterization of monoclonal antibodies specific for lion interferon-gamma (IFN-γ). Recombinant lion IFN-γ (rLIFN-γ) was produced in mammalian cells and used to immunize mice to establish hybridoma cell lines producing monoclonal antibodies. These were used to develop a sensitive, lion IFN-γ-specific capture ELISA, able to detect rLIFN-γ to the level of 160 pg/ml. Recognition of native lion IFN-γ was shown in an initial assessment of supernatants of mitogen stimulated whole blood cultures of 11 known BTB-negative lions. In conclusion, the capture ELISA shows potential as a diagnostic assay for bovine tuberculosis in lions. Preliminary results also indicate the possible use of the test for other (feline) species. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Tumor markers kits development for use in radioimmunometric assays

    International Nuclear Information System (INIS)

    Ahmed, B.

    1997-01-01

    The immunoassays such as RIA and IRMA are now widely used through the world for the quantitation of a variety of substances in the biological fluid for their high sensibility and specificity which required simple equipments. These techniques are also very used in Algeria for an effective amelioration of public heath The assays kits of RIA/IRMA of thyroid hormones are the most used, followed by peptidic hormones, steroids hormones and IRMA Tumor Markers (T.M) kits. In spite of the important demand, of tumor markers kits for the diagnosis and follow up of cancers their use are always insufficient due to the high cost. The research contract programme proposed by IAEA on the theme 'The Developments of IRMA Tumor Markers Kits' of prostate specific Antigen (PSA) and Tissue Polypeptide Specific Antigen (TPS) will allowed us to produce locally with best quality-price, the main reagents for PSA and TPS IRMA assays kits for diagnosis and follow up the prostate and breast cancers which are very spready in the country. This report include the following points: Generalities on the use of tumor markers in Algeria, programme for the Development of the PSA IRMA assay (schedule of protocols applied for each reagents; annual planning for assessing the programme activities) and conclusion

  12. LSDS Development for Isotopic Fissile Assay in Spent Fuel

    International Nuclear Information System (INIS)

    Lee, Yong Deok; Park, Chang Je; Park, Geun Il; Lee, Jung Won; Song, Kee Chan

    2011-01-01

    As an option to reduce a spent fuel and reuse an existing fissile material in spent fuel, sodium fast reactor SFR program linked with pyro-processing is under development in KAERI. A uranium-TRU mixture through a pyro-process is used to fabricate SFR fuel. An assay of isotopic fissile content plays an important role in an optimum design of storage site and reuse of fissile materials of spent fuel. Lead slowing down spectrometer LSDS is being developed in KAERI to analyze isotopic fissile material content. LSDS has several features: direct fissile assay, near real time fissile assay, no influence from radiation background, fissile isotopic assay and applicable to spent fuel and recycled fuel. Based on the designed geometry, neutron energy resolution was investigated. The neutron energy spectrum was analyzed as well. Spent fuel emits large number of neutrons by spontaneous fission. Neutron generator must overcome the neutron background to get the pure fission signals from fissile materials. Neutron generator is planned to have compact system with one section electron linac which is easy maintenance, less cost and high neutron yield. The LSD has the power to resolve the fission characteristics from each fissile material. This feature can analyze the content of isotopic fissile. From 1keV to 0.1eV energy range, the energy resolution is enough to get the individual fissile fission signatures. The dominant fission signature is shown below 1eV for each fissile isotope. The neutron generation system with target was designed to get fission signals by fissile materials. The system was decided to overcome neutron backgrounds and to get good counting statistics. Finally, an accurate fissile material content will contribute to safety of spent fuel reuse in future nuclear energy system and optimum design of spent fuel storage site. Additionally, an accurate fissile material content will increase international transparence and credibility for the reuse of PWR spent fuel

  13. Wind energy technology developments

    DEFF Research Database (Denmark)

    Madsen, Peter Hauge; Hansen, Morten Hartvig; Pedersen, Niels Leergaard

    2014-01-01

    turbine blades and towers are very large series-produced components, which costs and quality are strongly dependent on the manufacturing methods. The industrial wind energy sector is well developed in Denmark, and the competitive advantage of the Danish sector and the potential for job creation...

  14. Technological development in fisheries management

    DEFF Research Database (Denmark)

    Eigaard, Ole Ritzau; Marchal, Paul; Gislason, Henrik

    2014-01-01

    Many marine fish stocks are overexploited and considerable overcapacity exists in fishing fleets worldwide. One of the reasons for the imbalance between resource availability and fishing capacity is technological development, which continuously increases the efficiency of the vessels—a mechanism...... referred to as “technological creep.” We review how the introduction of new and more efficient electronic equipment, gear design, engines, deck equipment, and catch-handling procedures influences the capture efficiency (catchability) of commercial fishing vessels. On average, we estimate that catchability...... increases by 3.2% per year due to technological developments, an increase often ignored in fisheries management. The documentation and quantification of technological creep improves the basis for successfully integrating the effects of technological development (and catchability changes) in fisheries...

  15. Nigerian Journal of Technological Development

    African Journals Online (AJOL)

    The Nigerian Journal of Technological Development is now a quarterly publication of the Faculty of Engineering & Technology, University of Ilorin, Ilorin, Nigeria. It publishes original high-quality articles focusing on all aspects of Engineering and Applied Sciences in March, June, September and December. Manuscripts are ...

  16. High performance fuel technology development

    Energy Technology Data Exchange (ETDEWEB)

    Koon, Yang Hyun; Kim, Keon Sik; Park, Jeong Yong; Yang, Yong Sik; In, Wang Kee; Kim, Hyung Kyu [KAERI, Daejeon (Korea, Republic of)

    2012-01-15

    {omicron} Development of High Plasticity and Annular Pellet - Development of strong candidates of ultra high burn-up fuel pellets for a PCI remedy - Development of fabrication technology of annular fuel pellet {omicron} Development of High Performance Cladding Materials - Irradiation test of HANA claddings in Halden research reactor and the evaluation of the in-pile performance - Development of the final candidates for the next generation cladding materials. - Development of the manufacturing technology for the dual-cooled fuel cladding tubes. {omicron} Irradiated Fuel Performance Evaluation Technology Development - Development of performance analysis code system for the dual-cooled fuel - Development of fuel performance-proving technology {omicron} Feasibility Studies on Dual-Cooled Annular Fuel Core - Analysis on the property of a reactor core with dual-cooled fuel - Feasibility evaluation on the dual-cooled fuel core {omicron} Development of Design Technology for Dual-Cooled Fuel Structure - Definition of technical issues and invention of concept for dual-cooled fuel structure - Basic design and development of main structure components for dual- cooled fuel - Basic design of a dual-cooled fuel rod.

  17. Investigation of the antimicrobial activity of soy peptides by developing a high throughput drug screening assay

    OpenAIRE

    Dhayakaran, Rekha; Neethirajan, Suresh; Weng, Xuan

    2016-01-01

    Background Antimicrobial resistance is a great concern in the medical community, as well as food industry. Soy peptides were tested against bacterial biofilms for their antimicrobial activity. A high throughput drug screening assay was developed using microfluidic technology, RAMAN spectroscopy, and optical microscopy for rapid screening of antimicrobials and rapid identification of pathogens. Methods Synthesized PGTAVFK and IKAFKEATKVDKVVVLWTA soy peptides were tested against Pseudomonas aer...

  18. After TGN1412: recent developments in cytokine release assays.

    Science.gov (United States)

    Stebbings, R; Eastwood, D; Poole, S; Thorpe, R

    2013-01-01

    The failure of regulatory science to keep pace with and support the development of new biological medicines was very publically highlighted in March 2006 when the first-in-man Phase I clinical trial of the immunomodulatory CD28-specific monoclonal antibody (mAb) TGN1412 ended in disaster when all six volunteers suffered a life-threatening adverse reaction termed a 'Cytokine Storm'. The poor predictive value of standard pre-clinical safety tests and animal models applied to TGN1412 demonstrated the need for a new generation of immunotoxicity assays and animal models that are both sensitive and predictive of clinical outcome in man. The non-predictive result obtained from pre-clinical safety testing in cynomolgus macaques has now been attributed to a lack of CD28 expression on CD4+ effector memory T-cells that therefore cannot be stimulated by TGN1412. In contrast, high levels of CD28 are expressed on human CD4+ effector memory T-cells, the source of most TGN1412-stimulated pro-inflammatory cytokines. Standard in vitro safety tests with human cells were also non-predictive as they did not replicate in vivo presentation of TGN1412. It was subsequently shown that, if an immobilized therapeutic mAb-based assay or endothelial cell co-culture assay was used to evaluate TGN1412, then these would have predicted a pro-inflammatory response in man. New in vitro assays based on these approaches are now being applied to emerging therapeutics to hopefully prevent a repeat of the TGN1412 incident. It has emerged that the mechanism of pro-inflammatory cytokine release stimulated by TGN1412 is different to that of other therapeutic mAbs, such that standard pro-inflammatory markers such as TNFα and IL-8 are not discriminatory. Rather, IL-2 release and lymphoproliferation are optimal readouts of a TGN1412-like pro-inflammatory response.

  19. Multichip module technology development

    International Nuclear Information System (INIS)

    Kapustinsky, J.S.; Boissevain, J.G.; Muck, R.C.; Smith, G.D.; Wong-Swanson, B.G.; Ziock, H.J.

    1997-01-01

    This is the final report of a one-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). A Multichip Module (MCM) was designed and submitted for fabrication to the Lockheed Martin foundry using a licensed process called High Density Interconnect (HDI). The HDI process uses thin film techniques to create circuit interconnect patterns on multiple layers of dielectric film which are deposited directly on top of unpackaged electronic die. This results in an optimally small package that approaches the area of the bare die themselves. This project tested the capability of the Lockheed Martin foundry to produce, in an HDI process, a complex mixed-mode (analog and digital) circuit on a single MCM substrate

  20. Radioisotope Power Systems Technology Development

    Data.gov (United States)

    National Aeronautics and Space Administration — The goal of the RPS's technology portfolio is to advance performance of radioisotope power systems through new and novel innovations being developed and transitioned...

  1. Energy consumption and technological developments

    International Nuclear Information System (INIS)

    Okorokov, V.R.

    1990-02-01

    The paper determines an outline of the world energy prospects based on principal trends of the development of energy consumption analysed over the long past period. According to the author's conclusion the development of energy systems will be determined in the nearest future (30 - 40 years) by contemporary energy technologies based on the exploitation of traditional energy resources but in the far future technologies based on the exploitation of thermonuclear and solar energy will play the decisive role. (author)

  2. Development of Radioisotope Tracer Technology

    International Nuclear Information System (INIS)

    Jung, Sung Hee; Jin, Joon Ha; Kim, Jong Bum; Kim, Jin Seop; Kim, Jae Jo; Park, Soon Chul; Lim, Don Soon; Choi, Byung Jong; Jang, Dong Soon; Kim, Hye Sook

    2007-06-01

    The project is aimed to develop the radiotracer technology for process optimization and trouble-shooting to establish the environmental and industrial application of radiation and radioisotopes. The advanced equipment and software such as high speed data acquisition system, RTD model and high pressure injection tool have developed. Based on the various field application to the refinery/petrochemical industries, the developed technology was transfer to NDT company for commercial service. For the environmental application of radiotracer technology, injector, detector sled, core sampler, RI and GPS data logging system are developed and field tests were implemented successfully at Wolsung and Haeundae beach. Additionally tracer technology were also used for the performance test of the clarifier in a wastewater treatment plant and for the leak detection in reservoirs. From the experience of case studies on radiotracer experiment in waste water treatment facilities, 'The New Excellent Technology' is granted from the ministry of environment. For future technology, preliminary research for industrial gamma transmission and emission tomography which are new technology combined with radioisotope and image reconstruction are carried out

  3. Transmission blocking malaria vaccines: Assays and candidates in clinical development.

    Science.gov (United States)

    Sauerwein, R W; Bousema, T

    2015-12-22

    Stimulated by recent advances in malaria control and increased funding, the elimination of malaria is now considered to be an attainable goal for an increasing number of malaria-endemic regions. This has boosted the interest in transmission-reducing interventions including vaccines that target sexual, sporogenic, and/or mosquito-stage antigens to interrupt malaria transmission (SSM-VIMT). SSM-VIMT aim to prevent human malaria infection in vaccinated communities by inhibiting parasite development within the mosquito after a blood meal taken from a gametocyte carrier. Only a handful of target antigens are in clinical development and progress has been slow over the years. Major stumbling blocks include (i) the expression of appropriately folded target proteins and their downstream purification, (ii) insufficient induction of sustained functional blocking antibody titers by candidate vaccines in humans, and (iii) validation of a number of (bio)-assays as correlate for blocking activity in the field. Here we discuss clinical manufacturing and testing of current SSM-VIMT candidates and the latest bio-assay development for clinical evaluation. New testing strategies are discussed that may accelerate the evaluation and application of SSM-VIMT. Copyright © 2015. Published by Elsevier Ltd.

  4. Development of a chemiluminescence-based ribonuclease protection assay.

    Science.gov (United States)

    Rosenau, Christopher; Kaboord, Barbara; Qoronfleh, M Walid

    2002-12-01

    The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs are traditionally performed using radiolabeled probes that often require gel purification steps and lengthy exposure times to visualize results. Moreover, these probes can only be used for 1-2 weeks because of their short isotopic half-life and radiolysis. We report a method that improves the traditional RPA by replacing radiolabeled probes with biotinylated probes and lengthy exposure times with quick, streptavidin/HRP-based chemiluminescent detection technology. Biotinylated probes can be used without get purification and are stable for years, as opposed to weeks. Most importantly, our streptavidin/HRP-based chemiluminescent technology enables us to achieve sensitivity results similar to radioactive RPAs and to detect multiple transcripts in a single sample more efficiently. Furthermore, this new protocol addresses and eliminates the one major drawback unique to using biotinylated probes in chemiluminescent RPAs: a confounding artifact, not seen when running radioactive RPAs but commonly detected when using certain biotinylated rare message probes.

  5. Engineering research, development and technology

    International Nuclear Information System (INIS)

    1994-05-01

    The mission of the Engineering Research, Development, and Technology Program at Lawrence Livermore National Laboratory (LLNL) is to develop the technical staff, tools, and facilities needed to support current and future LLNL programs. The efforts are guided by a dual-benefit research and development strategy that supports Department of Energy missions, such as national security through nuclear deterrence and economic competitiveness through partnerships with U.S. industry. This annual report, organized by thrust area, describes the activities for the fiscal year 1993. The report provides timely summaries of objectives, methods, and results from nine thrust areas for this fiscal year: Computational Electronics and Electromagnetics; Computational Mechanics; Diagnostics and Microelectronics; Fabrication Technology; Materials Science and Engineering; Power Conversion Technologies; Nondestructive Evaluation; Remote Sensing, Imaging, and Signal Engineering; and Emerging Technologies. Separate abstracts were prepared for 47 papers in this report

  6. Development of versatile universal reagent immunoradiometric assay technique

    International Nuclear Information System (INIS)

    Hazra, D.K.

    1982-10-01

    Immunoradiometric assays, which make use of labelled antibodies, potentially offer better sensitivity and specificity than do radioimmunoassays, which use labelled antigens. In addition, they can in principle be performed in a particularly convenient scheme wherein the same labelled reagent may be used for many different analytes - thus serving as a ''universal'' labelled reagent. Thus if the specific antibody for every analyte is raised in rabbits, and an anti-rabbit antibody is labelled, the latter may be added after the specific antibody to quantify the amount of specific antibody bound to analyte and thereby the amount of analyte present. The potential greater sensitivity and specificity of the immunoradiometric procedure, coupled with the potential convenience of the ''universal'' labelled reagent, might allow such immunoradiometric techniques to be used effectively in the study of communicable diseases in developing countries. Development of these procedures was the subject of this investigation. Many components of these procedures had to be explored and provisionally optimized, including coating of assay tubes with ''extraction'' antibody, immunological purification of antibodies, labelling of antibodies, and intermediate steps toward these goals. Applications were thereupon tested using those provisionally optimized components. The ''universal'' labelled reagent, a donkey anti-rabbit antiserum, was successfully used in the assay of TSH; however, cross reactions of the reagent with non-rabbit immunoglobulins and other materials present seriously limited the sensitivity of the method. Using conventional immunoradiometric procedures, circulating TB and amoebic antibodies could be detected in patients suffering from these diseases. Similarly, circulating antigens in the same patients could also be detected, but not with sufficient sensitivity and specificity to provide a reliable analytical system. Numerous improvements will be required before these techniques

  7. The EM technology development strategy

    International Nuclear Information System (INIS)

    Frank, C.W.; Barainca, M.; Kubo, A.S.

    1992-01-01

    The Office of Technology Development (TD) supports research and development of technologies that will lower cost, reduce risk, improve safety, and accelerate cleanup of the Nuclear Weapons Complex and provide solutions to currently untractable environmental problems. The TD strategic plan outlines Applied Research, Development, Demonstration, Testing, and Evaluation (RDDT and E) that will provide needed technology products to be used by Environmental Restoration and Waste Management operations (i.e., our customers). The TD strategic plan is derived from EM Goals, Objectives, and Strategy and is incorporated into DOE'S Five-Year Plan for Environmental Restoration and Waste Management. The TD strategic plan is developed based on integrating customer requirements, and is complemented by a top-down, bottom-up analysis of Site Specific Technology Needs and environmental problems. The execution of TD's strategic plan is implemented largely through Integrated Programs (IP) and Integrated Demonstrations (ID). IDs have proven to be a cost-effective method of managing technology development, testing and evaluation, and implementation of successful technology systems into the DOE Environmental Restoration and Waste Management Programs. The Savannah River ID for Volatile Organic Compounds (VOCs) in Saturated Soils resulted in a 51 percent cost savings over stand-alone demonstrations, saving over $8 million. The IPs and IDs are selected based on customer needs, technical complexity, and complex-wide regulatory and compliance agreements. New technology systems are selected for incorporation into an IP or ID from offerings of the DOE laboratories, industry, and the universities. A major TD initiative was announced in August 1991, with the release of a Program Research and Development Announcement (PRDA) requesting industry and universities to propose innovative new technologies to clean up the Weapons Complex. (author)

  8. The development of information technologies

    Directory of Open Access Journals (Sweden)

    Kostúr Karol

    2002-12-01

    Full Text Available The contribution analyses the tasks information technologies in industry. At present time is the problem of connecting mutual variously levels of management. Therefore, first priority is an integration of information technologies. The information technologies for enterprise management are analysed. The product SAP R/3 appears as suitable for top management. The SAP R/3 Enterprise has a new technology so call web-server. This server enables the integration with e-business. The development my SAP is based on application of Business information warehouse (BW and Strategic enterprise management (SEM. The cheaper products (SPIN + EVIS are characterized too. Whitness Miner is very interesting system. The contribution this technology is programming realization of method KDD – knowledge discovery in databases.

  9. Development, validation and quantitative assessment of an enzymatic assay suitable for small molecule screening and profiling: A case-study

    Directory of Open Access Journals (Sweden)

    Vicente Sancenon

    2015-06-01

    Full Text Available The successful discovery and subsequent development of small molecule inhibitors of drug targets relies on the establishment of robust, cost-effective, quantitative, and physiologically relevant in vitro assays that can support prolonged screening and optimization campaigns. The current study illustrates the process of developing and validating an enzymatic assay for the discovery of small molecule inhibitors using alkaline phosphatase from bovine intestine as model target. The assay development workflow includes an initial phase of optimization of assay materials, reagents, and conditions, continues with a process of miniaturization and automation, and concludes with validation by quantitative measurement of assay performance and signal variability. The assay is further evaluated for dose–response and mechanism-of-action studies required to support structure–activity-relationship studies. Emphasis is placed on the most critical aspects of assay optimization and other relevant considerations, including the technology, assay materials, buffer constituents, reaction conditions, liquid handling equipment, analytical instrumentation, and quantitative assessments. Examples of bottlenecks encountered during assay development and strategies to address them are provided.

  10. Titanium Aluminide Casting Technology Development

    Science.gov (United States)

    Bünck, Matthias; Stoyanov, Todor; Schievenbusch, Jan; Michels, Heiner; Gußfeld, Alexander

    2017-12-01

    Titanium aluminide alloys have been successfully introduced into civil aircraft engine technology in recent years, and a significant order volume increase is expected in the near future. Due to its beneficial buy-to-fly ratio, investment casting bears the highest potential for cost reduction of all competing production technologies for TiAl-LPTB. However, highest mechanical properties can be achieved by TiAl forging. In view of this, Access e.V. has developed technologies for the production of TiAl investment cast parts and TiAl die cast billets for forging purposes. While these parts meet the highest requirements, establishing series production and further optimizing resource and economic efficiency are present challenges. In order to meet these goals, Access has recently been certified according to aircraft standards, aiming at qualifying parts for production on technology readiness level 6. The present work gives an overview of the phases of development and certification.

  11. Comaparison of Web Developement Technologies

    OpenAIRE

    Ramesh Nagilla, Ramesh

    2012-01-01

    Web applications play an important role for many business purpose activities in the modernworld. It has become a platform for the companies to fulfil the needs of their business. In thissituation, Web technologies that are useful in developing these kinds of applications become animportant aspect. Many Web technologies like Hypertext Preprocessor (PHP), Active ServerPages (ASP.NET), Cold Fusion Markup Language (CFML), Java, Python, and Ruby on Rails areavailable in the market. All these techn...

  12. European innovation and technology development

    International Nuclear Information System (INIS)

    Johnson, R.W.

    1991-01-01

    The promotion of technological innovation by European national governments and the EC in pursuit of both increased recovery and the anchoring of technology in supply, manufacturing and service sector companies has been a feature of the strategic involvement by European states in exploration and production research and development. This paper summaries past trends in this activity and reviews the targets for future industry innovation which will enable European (primarily the North Sea) production to be sustained for a further generation

  13. A flow cytometric assay technology based on quantum dots-encoded beads

    International Nuclear Information System (INIS)

    Wang Haiqiao; Liu Tiancai; Cao Yuancheng; Huang Zhenli; Wang Jianhao; Li Xiuqing; Zhao Yuandi

    2006-01-01

    A flow cytometric detecting technology based on quantum dots (QDs)-encoded beads has been described. Using this technology, several QDs-encoded beads with different code were identified effectively, and the target molecule (DNA sequence) in solution was also detected accurately by coupling to its complementary sequence probed on QDs-encoded beads through DNA hybridization assay. The resolution of this technology for encoded beads is resulted from two longer wavelength fluorescence identification signals (yellow and red fluorescent signals of QDs), and the third shorter wavelength fluorescence signal (green reporting signal of fluorescein isothiocyanate (FITC)) for the determination of reaction between probe and target. In experiment, because of QDs' unique optical character, only one excitation light source was needed to excite the QDs and probe dye FITC synchronously comparing with other flow cytometric assay technology. The results show that this technology has present excellent repeatability and good accuracy. It will become a promising multiple assay platform in various application fields after further improvement

  14. Application of expert system technology to nondestructive waste assay - initial prototype model

    International Nuclear Information System (INIS)

    Becker, G.K.; Determan, J.C.

    1997-01-01

    Expert system technology has been identified as a technique useful for filling certain types of technology/capability gaps in existing waste nondestructive assay (NDA) applications. In particular, expert system techniques are being investigated with the intent of providing on-line evaluation of acquired data and/or directed acquisition of data in a manner that mimics the logic and decision making process a waste NDA expert would employ. The space from which information and data sources utilized in this process is much expanded with respect to the algorithmic approach typically utilized in waste NDA. Expert system technology provides a mechanism to manage and reason with this expanded information/data set. The material presented in this paper concerns initial studies and a resultant prototype expert system that incorporates pertinent information, and evaluation logic and decision processes, for the purpose of validating acquired waste NDA measurement assays. 6 refs., 6 figs

  15. Development of Radiochemical Separation Technology

    International Nuclear Information System (INIS)

    Lee, Eil Hee; Kim, K. W.; Yang, H. B.

    2007-06-01

    This project of the second phase was aimed at the development of basic unit technologies for advanced partitioning, and the application tests of pre-developed partitioning technologies for separation of actinides by using a simulated multi-component radioactive waste containing Am, Np, Tc, U and so on. The goals for recovery yield of TRU, and for purity of Tc are high than 99% and about 99%, respectively. The work scopes and contents were as follows. 1). For the development of basic unit technologies for advanced partitioning. 1. Development of technologies for co-removal of TRU and for mutual separation of U and TRU with a reduction-complexation reaction. 2. Development of extraction system for high-acidity co-separation of An(+3) and Ln(+3) and its radiolytic evaluation. 3. Synthesis of extractants for the selective separation of An(+3) and its relevant extraction system development. 4. Development of a hybrid system for the recovery of noble metals and its continuous separation tests. 5. Development of electrolytic system for the decompositions of N-NO3 and N-NH3 compounds to nitrogen gas. 2). For the application test of pre-developed partitioning technologies for the separation of actinide elements in a simulated multi-component solution equivalent to HLW level. 1. Co-separation of Tc, Np and U by a (TBP-TOA)/NDD system. 2. Mutual-separation of Am, Cm and RE elements by a (Zr-DEHPA)/NDD system. All results will be used as the fundamental data for the development of advanced partitioning process in the future

  16. Development of Radiochemical Separation Technology

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eil Hee; Kim, K. W.; Yang, H. B. (and others)

    2007-06-15

    This project of the second phase was aimed at the development of basic unit technologies for advanced partitioning, and the application tests of pre-developed partitioning technologies for separation of actinides by using a simulated multi-component radioactive waste containing Am, Np, Tc, U and so on. The goals for recovery yield of TRU, and for purity of Tc are high than 99% and about 99%, respectively. The work scopes and contents were as follows. 1). For the development of basic unit technologies for advanced partitioning. 1. Development of technologies for co-removal of TRU and for mutual separation of U and TRU with a reduction-complexation reaction. 2. Development of extraction system for high-acidity co-separation of An(+3) and Ln(+3) and its radiolytic evaluation. 3. Synthesis of extractants for the selective separation of An(+3) and its relevant extraction system development. 4. Development of a hybrid system for the recovery of noble metals and its continuous separation tests. 5. Development of electrolytic system for the decompositions of N-NO3 and N-NH3 compounds to nitrogen gas. 2). For the application test of pre-developed partitioning technologies for the separation of actinide elements in a simulated multi-component solution equivalent to HLW level. 1. Co-separation of Tc, Np and U by a (TBP-TOA)/NDD system. 2. Mutual-separation of Am, Cm and RE elements by a (Zr-DEHPA)/NDD system. All results will be used as the fundamental data for the development of advanced partitioning process in the future.

  17. Experience of developing an integrated nondestructive assay system

    International Nuclear Information System (INIS)

    Hsue, S.T.; Baker, M.P.

    1987-01-01

    A consortium of laboratories is collaborating with the Savannah River Plant to develop an integrated system of state-of-the-art nondestructive assay (NDA) instrumentation to provide nuclear materials accounting and process control information for a new plutonium scrap recovery facility. Individual instruments report assay results to an instrument control computer (ICC); the ICC, in turn, is part of a larger computer network that includes computers that perform process control and materials accounting functions. The design of the integrated NDA measurement system is shown. Each NDA instrument that is part of the integrated system is microcomputer-based and thus is capable of stand-alone operation if the central computer is out of service. Certain hardware features, such as microcomputers, pulse processing modules, and multichannel analyzers, are standardized throughout the system. Another standard feature is the communication between individual NDA instruments and the ICC. The most unique phase of the project is the integral staging. The primary purpose of this phase is to check the communications between various computers and to verify the ICC software during the operation of the NDA instruments. Implementing this integrated system in a process environment represents a major step in realizing the full capabilities of modern NDA instrumentation

  18. Development of recombinant human IgA for anticardiolipin antibodies assay standardization.

    Science.gov (United States)

    Knappik, Achim; Capuano, Francesco; Frisch, Christian; Ylera, Francisco; Bonelli, Fabrizio

    2009-09-01

    Controls and calibrators in autoimmune assays are typically developed from patient sera. However, the use of sera is accompanied by a number of disadvantages, such as lack of monospecificity, lack of assay comparability, and supply limitations. Ideally, the control reagent would be an antigen-specific human monoclonal antibody preparation that is defined and pure, easy to produce without any supply limitations, and of defined isotype (IgG, IgM, or IgA). The generation of antigen-specific human monoclonal antibodies has been complicated, but recent advances in development of fully human antibodies by means of in vitro antibody gene library selection has opened a way for the isolation of human antibodies to virtually any antigen, including self-antigens. Such antibodies can be converted to any isotype by gene cloning. Here we developed a set of human monoclonal IgA antibodies specific for the cardiolipin-beta2-glycoprotein 1 complex, using the HuCAL technology. We evaluated the IgA variants of those antibodies for their use as standards in IgA anticardiolipin antibody assays and compared these reagents with serum controls. Such recombinant antibodies may ultimately replace patient sera as assay control and calibration reagents.

  19. Integrating Product and Technology Development

    DEFF Research Database (Denmark)

    Meijer, Ellen Brilhuis; Pigosso, Daniela Cristina Antelmi; McAloone, Tim C.

    2016-01-01

    .g. managing dependencies) and opportunities (e.g. streamlining development). This paper presents five existing reference models for technology development (TD), which were identified via a systematic literature review, where their possible integration with product development (PD) reference models......Although dual innovation projects, defined in this article as the concurrent development of products and technologies, often occur in industry, these are only scarcely supported methodologically. Limited research has been done about dual innovation projects and their inherent challenges (e...... was investigated. Based on the specific characteristics desired for dual innovation projects, such as integrated product development and coverage of multiple development stages, a set of selection criteria was employed to select suitable PD and TD reference models. The integration and adaptation of the selected...

  20. Space technology developments in Malaysia:

    Science.gov (United States)

    Sabirin, A.

    The venture of space is, by nature, a costly one. However, exploring space is not just an activity reserved for international superpowers. Smaller and emerging space nations, some with burgeoning space programs of their own, can play a role in space technology development and interplanetary exploration, sometimes simply by just being there. Over the past four decades, the range of services delivered by space technologies in Malaysia has grown enormously. For many business and public services, space based technologies have become the primary means of delivery of such services. Space technology development in Malaysia started with Malaysia's first microsatellite, TiungSAT-1. TiungSAT-1 has been successfully launched from the Baikonur Cosmodrome, Kazakhstan on the 26th of September 2000 on a Russian-Ukrainian Dnepr rocket. There have been wide imaging applications and information extraction using data from TiungSAT-1. Various techniques have been applied to the data for different applications in environmental assessment and monitoring as well as resource management. As a step forward, Malaysia has also initiated another space technology programme, RAZAKSAT. RAZAKSAT is a 180kg class satellite designed to provide 2.5meter ground sampling distance resolution imagery on a near equatorial orbit. Its mission objective is to demonstrate the capability of a medium high resolution remote sensing camera using a cost effective small satellite platform and a multi-channel linear push-broom electro-optical instrument. Realizing the immense benefits of space technology and its significant role in promoting sustainable development, Malaysia is committed to the continuous development and advancement of space technology within the scope of peaceful use of outer space and boosting its national economic growth through space related activities.

  1. Advanced Mirror & Modelling Technology Development

    Science.gov (United States)

    Effinger, Michael; Stahl, H. Philip; Abplanalp, Laura; Maffett, Steven; Egerman, Robert; Eng, Ron; Arnold, William; Mosier, Gary; Blaurock, Carl

    2014-01-01

    The 2020 Decadal technology survey is starting in 2018. Technology on the shelf at that time will help guide selection to future low risk and low cost missions. The Advanced Mirror Technology Development (AMTD) team has identified development priorities based on science goals and engineering requirements for Ultraviolet Optical near-Infrared (UVOIR) missions in order to contribute to the selection process. One key development identified was lightweight mirror fabrication and testing. A monolithic, stacked, deep core mirror was fused and replicated twice to achieve the desired radius of curvature. It was subsequently successfully polished and tested. A recently awarded second phase to the AMTD project will develop larger mirrors to demonstrate the lateral scaling of the deep core mirror technology. Another key development was rapid modeling for the mirror. One model focused on generating optical and structural model results in minutes instead of months. Many variables could be accounted for regarding the core, face plate and back structure details. A portion of a spacecraft model was also developed. The spacecraft model incorporated direct integration to transform optical path difference to Point Spread Function (PSF) and between PSF to modulation transfer function. The second phase to the project will take the results of the rapid mirror modeler and integrate them into the rapid spacecraft modeler.

  2. Development of a radioreceptor assay for β2 adrenergic agonists

    International Nuclear Information System (INIS)

    Helbo, V.; Vandenbroeck, M.; Maghuin-Rogister, G.

    1994-01-01

    Several β 2 adrenergic agonists are illegally used as growth promoters in meat production. We have developed and evaluated a radioreceptor assay for the multianalyte detection of these compounds. The method is based on a competition for binding with receptors (plasma membranes prepared from bovine teat muscles) between a radioactive tracer ( 3 H-dihydroalprenolol) and β 2 agonist residues present in the samples. The method has been validated for three β 2 agonists (clenbuterol, mabuterol and cimaterol) in bovine urine samples. The detection limit (mean of ''blank'' values + 3 SEM) in urine was 2.4 ppb clenbuterol. Using this procedure, samples containing at least 5 ppb of clenbuterol, mabuterol or cimaterol could be identified as positive for the presence of β 2 agonists. (orig.) [de

  3. Technological Development and Fisheries Management

    NARCIS (Netherlands)

    Eigaard, O.R.; Marchal, P.; Gislason, H.; Rijnsdorp, A.D.

    2014-01-01

    Many marine fish stocks are overexploited and considerable overcapacity exists in fishing fleets worldwide. One of the reasons for the imbalance between resource availability and fishing capacity is technological development, which continuously increases the efficiency of the vessels—a mechanism

  4. Technology and Motor Ability Development

    Science.gov (United States)

    Wang, Lin; Lang, Yong; Luo, Zhongmin

    2014-01-01

    As a new member joining the technology family, active video games have been developed to promote physical exercise. This working-in-progress paper shares an ongoing project on examining the basic motor abilities that are enhanced through participating in commercially available active video games. [For the full proceedings see ED557181.

  5. Development of radioisotope tracer technology

    International Nuclear Information System (INIS)

    Jin, Joon Ha; Lee, Myun Joo; Jung, Sung Hee; Park, Soon Chul; Lim, Dong Soon; Kim, Jae Ho; Lee, Jae Choon; Lee, Doo Sung; Cho, Yong Suk; Shin, Sung Kuan

    2000-04-01

    The purpose of this study is to develop the radioisotope tracer technology, which can be used in solving industrial and environmental problems and to build a strong tracer group to support the local industries. In relation to the tracer technology in 1999, experiments to estimate the efficiencies of a sludge digester of a waste water treatment plant and a submerged biological reactor of a dye industry were conducted. As a result, the tracer technology for optimization of facilities related to wastewater treatment has been developed and is believed to contribute to improve their operation efficiency. The quantification of the experimental result was attempted to improve the confidence of tracer technology by ECRIN program which basically uses the MCNP simulation principle. Using thin layer activation technique, wear of tappet shim was estimated. Thin layer surface of a tappet shim was irradiated by proton beam and the correlation between the measured activity loss and the amount of wear was established. The equipment was developed to adjust the energy of proton which collides with the surface of tappet. The tracer project team has participated into the tracer test for estimating the efficiency of RFCC system in SK cooperation. From the experiment the tracer team has obtained the primary elements to be considered for judging the efficiency of RFCC unit. By developing the tracer techniques to test huge industrial units like RFCC, the tracer team will be able to support the local industries that require technical services to solve any urgent trouble. (author)

  6. competitive technologies for sustainable development

    International Nuclear Information System (INIS)

    Chriqui, Vincent; Bergougnoux, Jean; Hossie, Gaelle; Beeker, Etienne; Buba, Johanne; Delanoe, Julien; Ducos, Geraldine; Hilt, Etienne; Rigard-Cerison, Aude; Teillant, Aude; Auverlot, Dominique; Martinez, Elise; Dambrine, Fabrice; Roure, Francoise

    2012-08-01

    By letter dated 27 April 2011, the Director General of the Centre for Strategic Analysis, Vincent Chriqui, confided to Jean Bergougnoux, honorary president of the SNCF, Honorary General Director of EDF, the task of animating a reflection Prospective Technological Studies of the sectors of energy, transport and construction. This synthesis report, prepared with the assistance of rapporteurs Centre for Strategic Analysis, attempts to summarize and put into perspective all the work which show these specific reports. Admittedly some very complex issues still need supplements. It may therefore be useful to extend this work in a number of areas. Beyond its role in the competitiveness of a country, technological innovation is essential to provide appropriate responses to the challenges of our commitment to sustainable development in terms of economic growth, preservation of the environmental and social progress. Mission for Prospective Technological conducted by the Centre for Strategic Analysis has sought to clarify this dual problem by proposing a long-term vision for the energy, transport and construction. For each technology studied, it has attempted to assess both the possible contribution to sustainable development and the competitive potential of our country on the international scene. His work, chaired by Jean Bergougnoux have reviewed the technological advances that may occur in the coming decades in the sectors concerned. They examined the conditions for integration of these advances in systems and subsystems existing (or create) and the conditions of a mature technical, economic but also social. Wherever possible, two time horizons were identified: a medium-term horizon, 2030, for which we have a fairly clear vision of future developments and long-term horizon, 2050, which allows to consider jumps Scientists are still uncertain. Finally, the mission is interested in four transverse technologies involved consistently in the three study areas, which are likely to

  7. Development and validation of a generic fluorescent methyltransferase activity assay based on the Transcreener® AMP/GMP Assay

    Science.gov (United States)

    Klink, Tony A.; Staeben, Matt; Twesten, Kim; Kopp, Andrew L.; Kumar, Meera; Dunn, Rebecca Schall; Pinchard, Cori A.; Kleman-Leyer, Karen M.; Klumpp, Martin; Lowery, Robert G.

    2013-01-01

    Methylation is a ubiquitous covalent modification used to control the function of diverse biomolecules including hormones, neurotransmitters, xenobiotics, proteins, nucleic acids and lipids. Histone methyltransferases (HMTs) are currently of high interest as drug targets because of their role in epigenetic regulation, however most HMT assay methods are either not amenable to an HTS environment or are applicable to a limited number of enzymes. We developed a generic methyltransferase assay method using fluorescent immunodetection of AMP, which is formed from the MT reaction product S-adenosylhomocysteine in a dual enzyme coupling step. The detection range of the assay, its suitability for HTS, including stability of reagents following dispensing and after addition to reactions as well as the potential for interference from drug like molecules was investigated. In addition, the use of the assay for measuring inhibitor potencies with peptide or intact protein substrates was examined through pilot screening with selected reference enzymes including HMT G9a. By combining a novel enzymatic coupling step with the well characterized Transcreener® AMP/GMP assay, we have developed a robust HTS assay for HMTs which should be broadly applicable to other types of methyltransferases as well. PMID:21956169

  8. Technology development life cycle processes.

    Energy Technology Data Exchange (ETDEWEB)

    Beck, David Franklin

    2013-05-01

    This report and set of appendices are a collection of memoranda originally drafted in 2009 for the purpose of providing motivation and the necessary background material to support the definition and integration of engineering and management processes related to technology development. At the time there was interest and support to move from Capability Maturity Model Integration (CMMI) Level One (ad hoc processes) to Level Three. As presented herein, the material begins with a survey of open literature perspectives on technology development life cycles, including published data on %E2%80%9Cwhat went wrong.%E2%80%9D The main thrust of the material presents a rational expose%CC%81 of a structured technology development life cycle that uses the scientific method as a framework, with further rigor added from adapting relevant portions of the systems engineering process. The material concludes with a discussion on the use of multiple measures to assess technology maturity, including consideration of the viewpoint of potential users.

  9. [Development of Rapid Assay of Transthyretin in Whole Blood].

    Science.gov (United States)

    Sato, Shinpei; Noma, Katsura; Morimoto, Makoto; Nobori, Tsutomu

    2014-09-01

    Transthyretin (TTR), a rapid turnover protein with a half-life of 2 days, which is shorter than that of albumin, is considered to be a sensitive indicator of a patient's nutritional status. We have newly developed a rapid assay of TTR by immunochromatography (IC) using samples of whole blood as well as serum at volumes of 10 μL. The fundamental performance was evaluated using control sera. In addition, whole blood samples were measured by IC and the results were compared with measured levels of plasma samples by turbidimetry and those of sera by nephelometry. The limit of quantification was 8.3 mg/dL. The linearity was 8.7-35.4 mg/dL. The reproducibility was 6.1-8.6% coefficient of variation. Bilirubin, hemolysis, chyle, and rheumatoid factor did not influence the measured levels. An increase of 1% in hematocrit resulted in a decrease of 1% in the measured levels. The correlation between IC (y) and nephelometry (x) was determined to be y = 0.927x+2.62 (r = 0.935, n = 64). The newly developed IC showed good performance and it was suggested that IC would be useful for nutrition monitoring as point of care test.

  10. Call for Applications – Clinical Assay Development Program | Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    The NCI Clinical Assay Development Program (CADP) is requesting project applications from investigators seeking clinical assay validation resources.  These resources are designed to assist with the development of assays that may predict therapy response or prognostic behavior of a diagnosed cancer, primarily for use in clinical trials. Approved projects for the NCI CADP will be provided access to the Institute’s assay development and validation resources, including project management support.

  11. Advanced Modular Inverter Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Adam Szczepanek

    2006-02-04

    Electric and hybrid-electric vehicle systems require an inverter to convert the direct current (DC) output of the energy generation/storage system (engine, fuel cells, or batteries) to the alternating current (AC) that vehicle propulsion motors use. Vehicle support systems, such as lights and air conditioning, also use the inverter AC output. Distributed energy systems require an inverter to provide the high quality AC output that energy system customers demand. Today's inverters are expensive due to the cost of the power electronics components, and system designers must also tailor the inverter for individual applications. Thus, the benefits of mass production are not available, resulting in high initial procurement costs as well as high inverter maintenance and repair costs. Electricore, Inc. (www.electricore.org) a public good 501 (c) (3) not-for-profit advanced technology development consortium assembled a highly qualified team consisting of AeroVironment Inc. (www.aerovironment.com) and Delphi Automotive Systems LLC (Delphi), (www.delphi.com), as equal tiered technical leads, to develop an advanced, modular construction, inverter packaging technology that will offer a 30% cost reduction over conventional designs adding to the development of energy conversion technologies for crosscutting applications in the building, industry, transportation, and utility sectors. The proposed inverter allows for a reduction of weight and size of power electronics in the above-mentioned sectors and is scalable over the range of 15 to 500kW. The main objective of this program was to optimize existing AeroVironment inverter technology to improve power density, reliability and producibility as well as develop new topology to reduce line filter size. The newly developed inverter design will be used in automotive and distribution generation applications. In the first part of this program the high-density power stages were redesigned, optimized and fabricated. One of the main

  12. Development of novel IVD assays: a manufacturer's perspective.

    Science.gov (United States)

    Metcalfe, Thomas A

    2010-01-01

    IVD manufacturers are heavily reliant on novel IVD assays to fuel their growth and drive innovation within the industry. They represent a key part of the IVD industry's value proposition to customers and the healthcare industry in general, driving product differentiation, helping to create demand for new systems and generating incremental revenue. However, the discovery of novel biomarkers and their qualification for a specific clinical purpose is a high risk undertaking and the large, risky investments associated with doing this on a large scale are incompatible with IVD manufacturer's business models. This article describes the sources of novel IVD assays, the processes for discovering and qualifying novel assays and the reliance of IVD manufacturers on collaborations and in-licensing to source new IVD assays for their platforms.

  13. EAGLE: relay mirror technology development

    Science.gov (United States)

    Hartman, Mary; Restaino, Sergio R.; Baker, Jeffrey T.; Payne, Don M.; Bukley, Jerry W.

    2002-06-01

    EAGLE (Evolutionary Air & Space Global Laser Engagement) is the proposed high power weapon system with a high power laser source, a relay mirror constellation, and the necessary ground and communications links. The relay mirror itself will be a satellite composed of two optically-coupled telescopes/mirrors used to redirect laser energy from ground, air, or space based laser sources to distant points on the earth or space. The receiver telescope captures the incoming energy, relays it through an optical system that cleans up the beam, then a separate transmitter telescope/mirror redirects the laser energy at the desired target. Not only is it a key component in extending the range of DoD's current laser weapon systems, it also enables ancillary missions. Furthermore, if the vacuum of space is utilized, then the atmospheric effects on the laser beam propagation will be greatly attenuated. Finally, several critical technologies are being developed to make the EAGLE/Relay Mirror concept a reality, and the Relay Mirror Technology Development Program was set up to address them. This paper will discuss each critical technology, the current state of the work, and the future implications of this program.

  14. How does technological regime affect performance of technology development projects?

    NARCIS (Netherlands)

    Song, Michael; Hooshangi, Soheil; Zhao, Y. Lisa; Halman, Johannes I.M.

    2014-01-01

    In this study, we examine how technological regime affects the performance of technology development projects (i.e., project quality, sales, and profit). Technological regime is defined as the set of attributes of a technological environment where the innovative activities of firms take place.

  15. Development of DUPIC safeguards technology

    Energy Technology Data Exchange (ETDEWEB)

    Kim, H. D.; Ko, W. I.; Song, D. Y. [and others

    2000-03-01

    During the first phase of R and D program conducted from 1997 to 1999, nuclear material safeguards studies system were performed on the technology development of DUPIC safeguards system such as nuclear material measurement in bulk form and product form, DUPIC fuel reactivity measurement, near-real-time accountancy, and containment and surveillance system for effective and efficient implementation of domestic and international safeguards obligation. For the nuclear material measurement system, the performance test was finished and received IAEA approval, and now is being used in DUPIC Fuel Fabrication Facility(DFDF) for nuclear material accounting and control. Other systems being developed in this study were already installed in DFDF and being under performance test. Those systems developed in this study will make a contribution not only to the effective implementation of DUPIC safeguards, but also to enhance the international confidence build-up in peaceful use of spent fuel material. (author)

  16. Recent progress in developing proximity ligation assays for pathogen detection.

    Science.gov (United States)

    Greenwood, Christina; Johnson, Gemma; Dhillon, Harvinder S; Bustin, Stephen

    2015-01-01

    The effective management of infectious diseases depends on the early detection of the microbes responsible, since pathogens are most effectively eliminated in the initial stages of infection. Current immunodiagnostic methods lack the sensitivity for earliest possible diagnosis. Nucleic acid-based tests (NATs) are more sensitive, but the detection of microbial DNA does not definitively prove the presence of a viable microorganism capable of causing a given infection. Proximity assays combine the specificity of antibody-based detection of proteins with the sensitivity and dynamic range of NATs, and their use may allow earlier as well as more clinically relevant detection than is possible with current NATs or immunoassays. However, the full potential of proximity assays for pathogen detection remains to be fulfilled, mainly due to the challenges associated with identifying suitable antibodies and antibody combinations, sensitivity issues arising from non-specific interactions of proximity probes and the longer incubation times required to carry out the assays.

  17. Development of a VHH-Based Erythropoietin Quantification Assay

    DEFF Research Database (Denmark)

    Kol, Stefan; Beuchert Kallehauge, Thomas; Adema, Simon

    2015-01-01

    human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification...

  18. Development of a lion-specific interferon-gamma assay

    NARCIS (Netherlands)

    Maas, M.; Kooten, van P.J.S.; Schreuder, J.; Morar, D.; Tijhaar, E.; Michel, A.L.; Rutten, V.P.M.G.

    2012-01-01

    The ongoing spread of bovine tuberculosis (BTB) in African free-ranging lion populations, for example in the Kruger National Park, raises the need for diagnostic assays for BTB in lions. These, in addition, would be highly relevant for zoological gardens worldwide that want to determine the BTB

  19. Development of nuclear analytical technology

    International Nuclear Information System (INIS)

    Jee, Kwang Yong; Kim, W. H.; Park, Yeong J.; Park, Yong J.; Sohn, S. C.; Song, B. C.; Jeon, Y. S.; Pyo, H. Y.; Ha, Y. K.

    2004-04-01

    The objectives of this study are to develop the technology for the determination of isotopic ratios of nuclear particles detected from swipe samples and to develop the NIPS system. The R and D contents and results of this study are firstly the production of nuclear micro particle(1 ∼ 20 μm) and standardization, the examination of variation in fission track characteristic according to nuclear particle size and enrichment( 235 U: 1-50%), the construction of database and the application of this technique to swipe samples. If this technique is verified its superiority by various field tests and inter-laboratory comparison program with other institutes in developed countries, it can be possible to join NWAL supervised under IAEA and to export our technology abroad. Secondly, characteristics of alpha track by boron (n, α) nuclear reaction were studied to measure both total boron concentration and 10B enrichment. The correlation of number of alpha tracks and various 10B concentration was studied to evaluate the reliability of this method. Especially, cadmium shielding technique was introduced to reduce the background of alpha tracks by covering the solid track detector and the multi-dot detector plate was developed to increase the reproducibility of measurement by making boron solution dried evenly in the plate. The results of the alpha track method were found to be well agreed with those of mass spectroscopy within less than 10 % deviation. Finally, the NIPS system using 252 Cf neutron source was developed and prompt gamma spectrum and its background were obtained. Monte Carlo method using MCNP-4B code was utilized for the interpretation of neutron and gamma-ray shielding condition as well as the moderation of a fast neutron. Gamma-gamma coincidence was introduced to reduce the prompt gamma background. The counting efficiency of the HPGe detector was calibrated in the energy range from 50 keV to 10 MeV using radio isotope standards and prompt gamma rays of Cl for the

  20. Dry rod consolidation technology development

    International Nuclear Information System (INIS)

    Rasmussen, T.L.; Schoonen, D.H.; Feldman, E.M.; Fisher, M.W.

    1987-01-01

    The Department of Energy's (DOE) Office of Civilian Radioactive Waste Management (OCRWM) is funding a program to consolidate commercial spent fuel for testing in dry storage casks and to develop technology that will be fed into other OCRWM programs, e.g., Prototypical Consolidation Demonstration Program (PCDP). The program is being conducted at the Idaho National Engineering Laboratory (INEL) by the INEL Operating Contractor EG and G Idaho, Inc. Hardware and software have been designed and fabricated for installation in a hot cell adjacent to the Test Area North (TAN) Hot Shop Facility. This equipment is used to perform dry consolidation of commercial spent fuel from the Virginia Power (VP) Cooperative Agreement Spent Fuel Storage Cask (SFSC) Demonstration Program and assemblies that had previously been stored at the Engine Maintenance and Disassembly (EMAD) facility in Nevada. Consolidation is accomplished by individual, horizontal rod pulling. A computerized semiautomatic control system with operator involvement is utilized to conduct consolidation operations. During consolidation operations, data is taken to characterize this technology. Still photo, video tape, and other documentation will be generated to make developed information available to interested parties. Cold checkout of the hardware and software was completed in September of 1986. Following installation in the hot cell, consolidation operations begins in May 1987. Resulting consolidated fuel will be utilized in the VP Cooperative Agreement SFSC Program

  1. Dry rod consolidation technology development

    International Nuclear Information System (INIS)

    Rasmussen, T.L.; Schoonen, D.H.; Fisher, M.W.

    1986-01-01

    The Department of Energy's (DOE) Office of Civilian Radioactive Waste Management (OCRWM) is funding a Program to consolidate commercial spent fuel for testing in dry storage casks and to develop technology that will be fed into other OCRWM Programs, e.g., Prototypical Consolidation Demonstration Program. The Program is being conducted at the Idaho National Engineering Laboratory (INEL) by the Operating Contractor, EGandG Idaho, Inc. Hardware and software have been designed and fabricated for installation in a hot cell adjacent to the Test Area North (TAN) Hot Shop Facility. This equipment will be used to perform dry consolidation of commercial spent fuel from the Virginia Power (VP) Cooperative Agreement Spent Fuel Storage Cask (SPSC) Demonstration Program and assemblies that had previously been stored at the Engine Maintenance and Disassembly (EMAD) facility in Nevada. Consolidation will be accomplished by individual, horizontal rod pulling. A computerized semi-automatic control system with operator involvement will be utilized to conduct consolidation operations. Special features have been incorporated in the design to allow crud collection and measurement of rod pulling forces. During consolidation operations, data will be taken to characterize this technology. Still photo, video tape, and other documentation will be generated to make developed information available to interested parties. Cold checkout of the hardware and software will complete in September of 1986. Following installation in the hot cell, consolidation operations will begin in January 1987. Resulting consolidated fuel will be utilized in the VP Cooperative Agreement SFSC Program

  2. Development of a rapid, simple assay of plasma total carotenoids

    Science.gov (United States)

    2012-01-01

    Background Plasma total carotenoids can be used as an indicator of risk of chronic disease. Laboratory analysis of individual carotenoids by high performance liquid chromatography (HPLC) is time consuming, expensive, and not amenable to use beyond a research laboratory. The aim of this research is to establish a rapid, simple, and inexpensive spectrophotometric assay of plasma total carotenoids that has a very strong correlation with HPLC carotenoid profile analysis. Results Plasma total carotenoids from 29 volunteers ranged in concentration from 1.2 to 7.4 μM, as analyzed by HPLC. A linear correlation was found between the absorbance at 448 nm of an alcohol / heptane extract of the plasma and plasma total carotenoids analyzed by HPLC, with a Pearson correlation coefficient of 0.989. The average coefficient of variation for the spectrophotometric assay was 6.5% for the plasma samples. The limit of detection was about 0.3 μM and was linear up to about 34 μM without dilution. Correlations between the integrals of the absorption spectra in the range of carotenoid absorption and total plasma carotenoid concentration gave similar results to the absorbance correlation. Spectrophotometric assay results also agreed with the calculated expected absorbance based on published extinction coefficients for the individual carotenoids, with a Pearson correlation coefficient of 0.988. Conclusion The spectrophotometric assay of total carotenoids strongly correlated with HPLC analysis of carotenoids of the same plasma samples and expected absorbance values based on extinction coefficients. This rapid, simple, inexpensive assay, when coupled with the carotenoid health index, may be useful for nutrition intervention studies, population cohort studies, and public health interventions. PMID:23006902

  3. Information Communication Technology Planning in Developing Countries

    Science.gov (United States)

    Malapile, Sandy; Keengwe, Jared

    2014-01-01

    This article explores major issues related to Information Communication Technology (ICT) in education and technology planning. Using the diffusion of innovation theory, the authors examine technology planning opportunities and challenges in Developing countries (DCs), technology planning trends in schools, and existing technology planning models…

  4. Development and Demonstration of a Multiplexed Magnetic Tweezers Assay

    Science.gov (United States)

    Johnson, Keith Charles

    This dissertation is concerned with the methods and applications of single molecule force spectroscopy. In the introduction, the traditional single molecule force spectroscopy instruments are introduced and the advantages and drawbacks of each are discussed. The first chapter is a review of methods to ensure that biomolecular bond lifetime parameter estimations are not contaminated by multiple bond data. This review culminates in an examination of the literature on the strength of the bond between biotin and streptavidin and finds that by filtering the numerous publications for those that clearly demonstrate specific single bond behavior, there is a consensus of the bond strength and kinetic parameters. The second chapter of the dissertation discusses the capabilities of a magnetic tweezer assay, which combines massive multiplexing, precision bead tracking, and bi-directional force control into a flexible and stabile platform for examining single molecule behavior. Using a novel method for increasing the precision of force estimations on heterogeneous paramagnetic beads, I demonstrate the instrument by examining the force dependence of uncoiling and recoiling velocity of type 1 fimbriae from Eschericia coli (E. coli) bacteria, and see similar results to previous studies. Chapter 3 is a study of the lifetime of the activated FimH-mannose bond under various force conditions using the previously described magnetic tweezer. The bond is found to be extremely long-lived at forces less than 30 pN, with an average lifetime > 1000 times longer than the biotin-streptavidin bond, making it one of the strongest non-covalent interactions known in nature. Furthermore, the average lifetime of the bond is similar between 9 and 30 pN of force, suggesting a force range at which the lifetime is force-independent, demonstrating ideal bond behavior for the first time in a natural system. It is hypothesized that the long lifetime and ideal behavior is due to a gateway that locks mannose

  5. Inspection technologies -Development of national safeguards technology-

    International Nuclear Information System (INIS)

    Hong, J. S.; Kim, B. K.; Kwack, E. H.

    1996-12-01

    17 facility regulations prepared by nuclear facilities according to the Ministerial Notices were evaluated. Safeguards inspection activities under Safeguards are described. Safeguards inspection equipments and operation manuals to be used for national inspection are also described. Safeguards report are produced and submitted to MOST by using the computerized nuclear material accounting system at state level. National inspection support system are developed to produce the on-site information for domestic inspection. Planning and establishment of policy for nuclear control of nuclear materials, international cooperation for nuclear control, CTBT, strengthening of international safeguards system, and the supply of PWRs to North Korea are also described. (author). 43 tabs., 39 figs

  6. Advanced baffle materials technology development

    Science.gov (United States)

    Johnson, E. A.; Vonbenken, C. J.; Halverson, W. D.; Evans, R. D.; Wollam, J. S.

    1991-10-01

    Optical sensors for strategic defense will require optical baffles to achieve adequate off-axis stray light rejection and pointing accuracy. Baffle materials must maintain their optical performance after exposure to both operational and threat environments. In addition, baffle materials must not introduce contamination which would compromise the system signal-to-noise performance or impair system mission readiness. Critical examination of failure mechanisms in current baffle materials are quite fragile and contribute to system contamination problems. Spire has developed technology to texture the substrate directly, thereby, removing minute, fragile interfaces subject to mechanical failure. This program has demonstrated that ion beam texturing produces extremely dark surfaces which are immune to damage from ordinary handling. This technology allows control of surface texture feature size and hence the optical wavelength at which the surface absorbs. The USAMTL/Spire program has produced dramatic improvements in the reflectance of ion beam textured aluminum without compromising mechanical hardness. In simulated launch vibration tests, this material produced no detectable contamination on adjacent catcher plates.

  7. Comparison of functional assays used in the clinical development of a placental malaria vaccine.

    Science.gov (United States)

    Pehrson, Caroline; Heno, Kristine K; Adams, Yvonne; Resende, Mafalda; Mathiesen, Line; Soegaard, Max; de Jongh, Willem A; Theander, Thor G; Salanti, Ali; Nielsen, Morten A

    2017-01-23

    Malaria in pregnancy is associated with significant morbidity in pregnant women and their offspring. Plasmodium falciparum infected erythrocytes (IE) express VAR2CSA that mediates binding to chondroitin sulphate A (CSA) in the placenta. Two VAR2CSA-based vaccines for placental malaria are in clinical development. The purpose of this study was to evaluate the robustness and comparability of binding inhibition assays used in the clinical development of placental malaria vaccines. The ability of sera from animals immunised with different VAR2CSA constructs to inhibit IE binding to CSA was investigated in three in vitro assays using 96-well plates, petri dishes, capillary flow and an ex vivo placental perfusion assay. The inter-assay variation was not uniform between assays and ranged from above ten-fold in the flow assay to two-fold in the perfusion assay. The intra-assay variation was highest in the petri dish assay. A positive correlation between IE binding avidity and the level of binding after antibody inhibition in the petri dish assay indicate that high avidity IE binding is more difficult to inhibit. The highest binding inhibition sensitivity was found in the 96-well and petri dish assays compared to the flow and perfusion assays where binding inhibition required higher antibody titers. The inhibitory capacity of antibodies is not easily translated between assays and the high sensitivity of the 96-well and petri dish assays stresses the need for comparing serial dilutions of serum. Furthermore, IE binding avidity must be in the same range when comparing data from different days. There was an overall concordance in the capacity of antibody-mediated inhibition, when comparing the in vitro assays with the perfusion assay, which more closely represents in vivo conditions. Importantly the ID1-ID2a protein in a liposomal formulation, currently in a phase I trial, effectively induced antibodies that inhibited IE adhesion in placental tissue. Copyright © 2016

  8. Technology transfer and international development: Materials and manufacturing technology

    Science.gov (United States)

    1982-01-01

    Policy oriented studies on technological development in several relatively advanced developing countries were conducted. Priority sectors defined in terms of technological sophistication, capital intensity, value added, and export potential were studied in Brazil, Venezuela, Israel, and Korea. The development of technological policy alternatives for the sponsoring country is assessed. Much emphasis is placed on understanding the dynamics of the sectors through structured interviews with a large sample of firms in the leading manufacturing and materials processing sectors.

  9. Technology in Sustainable Development Context

    Science.gov (United States)

    Uno, Kimio

    The economic and demographic growth in Asia has put increased importance to this part of the world whose contribution to the global community is vital in meeting global challenges. International cooperation in engineering education assumes a pivotal role in providing access to the frontiers of scientific and technological knowledge to the growing youths in the region. The thrust for advancement has been provided by the logic coming from the academic world itself, whereas expectations are high that the engineering education responds to challenges that are coming from outside the universities, such as environmental management, disaster management, and provision of common knowledge platform across disciplinary lines. Some cases are introduced in curriculum development that incorporates fieldwork and laboratory work intended to enhance the ability to cooperate. The new mode is discussed with focus on production, screening, storing/delivery, and leaning phases of knowledge. The strength of shared information will be enhanced through international cooperation.

  10. Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates†

    Science.gov (United States)

    Bowers, Holly A.; Tengs, Torstein; Glasgow, Howard B.; Burkholder, JoAnn M.; Rublee, Parke A.; Oldach, David W.

    2000-01-01

    Pfiesteria complex species are heterotrophic and mixotrophic dinoflagellates that have been recognized as harmful algal bloom species associated with adverse fish and human health effects along the East Coast of North America, particularly in its largest (Chesapeake Bay in Maryland) and second largest (Albermarle-Pamlico Sound in North Carolina) estuaries. In response to impacts on human health and the economy, monitoring programs to detect the organism have been implemented in affected areas. However, until recently, specific identification of the two toxic species known thus far, Pfiesteria piscicida and P. shumwayae (sp. nov.), required scanning electron microscopy (SEM). SEM is a labor-intensive process in which a small number of cells can be analyzed, posing limitations when the method is applied to environmental estuarine water samples. To overcome these problems, we developed a real-time PCR-based assay that permits rapid and specific identification of these organisms in culture and heterogeneous environmental water samples. Various factors likely to be encountered when assessing environmental samples were addressed, and assay specificity was validated through screening of a comprehensive panel of cultures, including the two recognized Pfiesteria species, morphologically similar species, and a wide range of other estuarine dinoflagellates. Assay sensitivity and sample stability were established for both unpreserved and fixative (acidic Lugol's solution)-preserved samples. The effects of background DNA on organism detection and enumeration were also explored, and based on these results, we conclude that the assay may be utilized to derive quantitative data. This real-time PCR-based method will be useful for many other applications, including adaptation for field-based technology. PMID:11055905

  11. Global Air Mobility Advanced Technologies (GAMAT) Advanced Technology Development (ATD) Phase II Research and Development

    National Research Council Canada - National Science Library

    Kuper, Samuel R; Scott, Ronald; Kazmierczak, Thomas; Roth, Emilie; Whitaker, Randall

    2004-01-01

    ...) Advanced Technology Development (ATD) research and development program. The goal of the GAMAT ATD was to further the development of a new type of user interface technology called Work-Centered Support System (WCSS) technology. The U.S...

  12. Evaluation of a liquid dispenser for assay development and enzymology in 1536-well format.

    Science.gov (United States)

    Butendeich, Heike; Pierret, Noëlle M; Numao, Shin

    2013-06-01

    Although developments in liquid dispensers have made the use of 1536-well plates for high-throughput screening (HTS) standard, there is still a gap in dispenser technology for performing matrix experiments with several components. Experiments such as those performed during assay development and enzymological studies are therefore still performed by manual pipetting in lower-density plates. We have evaluated a new dispenser, the Certus liquid dispenser (Gyger Fluidics GmbH, Switzerland), that is capable of flexible dispensing in 1536-well format, with a dead volume of less than 200 µL. Taking advantage of the precision of the dispenser for volumes down to 50 nL, we have created concentration gradients on plates by dispensing different volumes of reagent and then backfilling with buffer. Using this method and the flexibility of the dispenser software, we have performed several multidimensional experiments varying two to three components, including an assay development for an HTS, a mode of inhibition study, and a cofactor optimization, in which we determined 32 KM values. Overall, the flexibility of the plate layout for multiple components, the accuracy to dispense volumes ranging 2 log orders, and minimal reagent usage enable this dispenser for complex biochemical experiments.

  13. Arctic Energy Technology Development Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Sukumar Bandopadhyay; Charles Chamberlin; Robert Chaney; Gang Chen; Godwin Chukwu; James Clough; Steve Colt; Anthony Covescek; Robert Crosby; Abhijit Dandekar; Paul Decker; Brandon Galloway; Rajive Ganguli; Catherine Hanks; Rich Haut; Kristie Hilton; Larry Hinzman; Gwen Holdman; Kristie Holland; Robert Hunter; Ron Johnson; Thomas Johnson; Doug Kame; Mikhail Kaneveskly; Tristan Kenny; Santanu Khataniar; Abhijeet Kulkami; Peter Lehman; Mary Beth Leigh; Jenn-Tai Liang; Michael Lilly; Chuen-Sen Lin; Paul Martin; Pete McGrail; Dan Miller; Debasmita Misra; Nagendra Nagabhushana; David Ogbe; Amanda Osborne; Antoinette Owen; Sharish Patil; Rocky Reifenstuhl; Doug Reynolds; Eric Robertson; Todd Schaef; Jack Schmid; Yuri Shur; Arion Tussing; Jack Walker; Katey Walter; Shannon Watson; Daniel White; Gregory White; Mark White; Richard Wies; Tom Williams; Dennis Witmer; Craig Wollard; Tao Zhu

    2008-12-31

    The Arctic Energy Technology Development Laboratory was created by the University of Alaska Fairbanks in response to a congressionally mandated funding opportunity through the U.S. Department of Energy (DOE), specifically to encourage research partnerships between the university, the Alaskan energy industry, and the DOE. The enabling legislation permitted research in a broad variety of topics particularly of interest to Alaska, including providing more efficient and economical electrical power generation in rural villages, as well as research in coal, oil, and gas. The contract was managed as a cooperative research agreement, with active project monitoring and management from the DOE. In the eight years of this partnership, approximately 30 projects were funded and completed. These projects, which were selected using an industry panel of Alaskan energy industry engineers and managers, cover a wide range of topics, such as diesel engine efficiency, fuel cells, coal combustion, methane gas hydrates, heavy oil recovery, and water issues associated with ice road construction in the oil fields of the North Slope. Each project was managed as a separate DOE contract, and the final technical report for each completed project is included with this final report. The intent of this process was to address the energy research needs of Alaska and to develop research capability at the university. As such, the intent from the beginning of this process was to encourage development of partnerships and skills that would permit a transition to direct competitive funding opportunities managed from funding sources. This project has succeeded at both the individual project level and at the institutional development level, as many of the researchers at the university are currently submitting proposals to funding agencies, with some success.

  14. Cooperative technology development: An approach to advancing energy technology

    International Nuclear Information System (INIS)

    Stern, T.

    1989-09-01

    Technology development requires an enormous financial investment over a long period of time. Scarce national and corporate resources, the result of highly competitive markets, decreased profit margins, wide currency fluctuations, and growing debt, often preclude continuous development of energy technology by single entities, i.e., corporations, institutions, or nations. Although the energy needs of the developed world are generally being met by existing institutions, it is becoming increasingly clear that existing capital formation and technology transfer structures have failed to aid developing nations in meeting their growing electricity needs. This paper will describe a method for meeting the electricity needs of the developing world through technology transfer and international cooperative technology development. The role of nuclear power and the advanced passive plant design will be discussed. (author)

  15. Robotics Technology Development Program Cross Cutting and Advanced Technology

    International Nuclear Information System (INIS)

    Harrigan, R.W.; Horschel, D.S.

    1994-01-01

    Need-based cross cutting technology is being developed which is broadly applicable to the clean up of hazardous and radioactive waste within the US Department of Energy's complex. Highly modular, reusable technologies which plug into integrated system architectures to meet specific robotic needs result from this research. In addition, advanced technologies which significantly extend current capabilities such as automated planning and sensor-based control in unstructured environments for remote system operation are also being developed and rapidly integrated into operating systems

  16. Impact of immunization technology and assay application on antibody performance--a systematic comparative evaluation.

    Directory of Open Access Journals (Sweden)

    Michael C Brown

    Full Text Available Antibodies are quintessential affinity reagents for the investigation and determination of a protein's expression patterns, localization, quantitation, modifications, purification, and functional understanding. Antibodies are typically used in techniques such as Western blot, immunohistochemistry (IHC, and enzyme-linked immunosorbent assays (ELISA, among others. The methods employed to generate antibodies can have a profound impact on their success in any of these applications. We raised antibodies against 10 serum proteins using 3 immunization methods: peptide antigens (3 per protein, DNA prime/protein fragment-boost ("DNA immunization"; 3 per protein, and full length protein. Antibodies thus generated were systematically evaluated using several different assay technologies (ELISA, IHC, and Western blot. Antibodies raised against peptides worked predominantly in applications where the target protein was denatured (57% success in Western blot, 66% success in immunohistochemistry, although 37% of the antibodies thus generated did not work in any of these applications. In contrast, antibodies produced by DNA immunization performed well against both denatured and native targets with a high level of success: 93% success in Western blots, 100% success in immunohistochemistry, and 79% success in ELISA. Importantly, success in one assay method was not predictive of success in another. Immunization with full length protein consistently yielded the best results; however, this method is not typically available for new targets, due to the difficulty of generating full length protein. We conclude that DNA immunization strategies which are not encumbered by the limitations of efficacy (peptides or requirements for full length proteins can be quite successful, particularly when multiple constructs for each protein are used.

  17. Open Technology Development: Roadmap Plan

    National Research Council Canada - National Science Library

    Herz, J. C; Lucas, Mark; Scott, John

    2006-01-01

    .... Collaborative and distributed online tools; and 4. Technological Agility. Open standards and interfaces were initially established through ARPA and distributed via open source software reference implementations...

  18. HTGR technology development: status and direction

    International Nuclear Information System (INIS)

    Kasten, P.R.

    1982-01-01

    During the last two years there has been an extensive and comprehensive effort expended primarily by General Atomic (GA) in generating a revised technology development plan. Oak Ridge National Laboratory (ORNL) has assisted in this effort, primarily through its interactions over the past years in working together with GA in technology development, but also through detailed review of the initial versions of the technology development plan as prepared by GA. The plan covers Fuel Technology, Materials Technology (including metals, graphite, and ceramics), Plant Technology (including methods, safety, structures, systems, heat exchangers, control and electrical, and mechanical), and Component Design Verification and Support areas

  19. Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis.

    Directory of Open Access Journals (Sweden)

    Khalid Shahin

    Full Text Available Francisella noatunensis subsp. orientalis (Fno is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality. Early and accurate detection of Fno is crucial to set appropriate outbreak control measures in tilapia farms. Laboratory detection of Fno mainly depends on bacterial culture and molecular techniques. Recombinase polymerase amplification (RPA is a novel isothermal technology that has been widely used for the molecular diagnosis of various infectious diseases. In this study, a recombinase polymerase amplification (RPA assay for rapid detection of Fno was developed and validated. The RPA reaction was performed at a constant temperature of 42°C for 20 min. The RPA assay was performed using a quantitative plasmid standard containing a unique Fno gene sequence. Validation of the assay was performed not only by using DNA from Fno, closely related Francisella species and other common bacterial pathogens in tilapia farms, but also by screening 78 Nile tilapia and 5 water samples. All results were compared with those obtained by previously established real-time qPCR. The developed RPA showed high specificity in detection of Fno with no cross-detection of either the closely related Francisella spp. or the other tested bacteria. The Fno-RPA performance was highly comparable to the published qPCR with detection limits at 15 and 11 DNA molecules detected, respectively. The RPA gave quicker results in approximately 6 min in contrast to the qPCR that needed about 90 min to reach the same detection limit, taking only 2.7-3 min to determine Fno in clinical samples. Moreover, RPA was more tolerant to reaction inhibitors than qPCR when tested with field samples. The fast reaction, simplicity, cost-effectiveness, sensitivity and specificity make the RPA an attractive diagnostic tool that will contribute to controlling the

  20. Technological development in mechanical ventilation.

    Science.gov (United States)

    Conti, Giorgio; Costa, Roberta

    2010-02-01

    Innovative modes of mechanical ventilation, mainly based on complex closed loop technologies, have been recently developed and are now available for clinical use. Proportional assist ventilation with load-adjustable gain factors and neurally adjusted ventilatory assist are innovative modes of mechanical ventilation delivering a level of assistance proportional to the patient's effort, thus improving patient-ventilator synchrony and potentially representing a real clinical advantage. Adaptive support ventilation is a ventilatory mode delivering assisted (pressure support ventilation-like) or controlled breathing cycles (pressure-controlled-like), related to a minute ventilation target set by the clinician and on automated measurements of the patient's respiratory mechanics. Noisy pressure support ventilation, finally, is a recently described experimental evolution of pressure support, with some improvement potentials, but no clinical application till now. The recently reported results with proportional assist ventilation with load-adjustable gain factors, neurally adjusted ventilatory assist, and adaptive support ventilation are, till now, mainly based on preliminary physiologic and clinical studies; although they seem to be promising, suggesting that closed loop-based modes could represent a real innovation in the field of mechanical ventilation, further clinical evaluation is needed before their widespread diffusion into clinical practice.

  1. Development of superconductor application technology

    Energy Technology Data Exchange (ETDEWEB)

    Hong, G. W.; Kim, C. J.; Lee, H. G.; Lee, H. J.; Kim, K. B.; Won, D. Y.; Jang, K. I.; Kwon, S. C.; Kim, W. J.; Ji, Y. A.; Yang, S. W.; Kim, W. K.; Park, S. D.; Lee, M. H.; Lee, D. M.; Park, H. W.; Yu, J. K.; Lee, I. S.; Kim, J. J.; Choi, H. S.; Chu, Y.; Kim, Y. S.; Kim, D. H.

    1997-09-01

    Fabrication of high Tc bulk superconductor and its application, fabrication of superconducting wire for electric power device and analysis for cryogenic system were carried out for developing superconductor application technologies for electric power system. High quality YBaCuO bulk superconductor was fabricated by controlling initial powder preparation process and prototype flywheel energy storage device was designed basically. The superconducting levitation force measuring device was made to examine the property of prepared superconductor specimen. Systematic studies onthe method of starting powder preparation, mechanical fabrication process, heat treatment condition and analysis of plastic deformation were carried out to increase the stability and reproducibility of superconducting wire. A starting power with good reactivity and fine particle size was obtained by mechanical grinding, control of phase assemblage, and emulsion drying method. Ag/BSCCO tape with good cross sectional shape and Jc of 20,000 A/cm{sup 2} was fabricated by applying CIP packing procedure. Multifilamentary wire with Jc of 10,000 A/cm{sup 2} was fabricated by rolling method using square billet as starting shape. The joining of the multifilamentary wire was done by etching and pressing process and showed 50% of joining efficiency. Analysis on the heat loss in cryostat for high Tc superconducting device was carried out for optimum design of the future cryogenic system. (author). 66 refs., 104 figs.

  2. Development of superconductor application technology

    International Nuclear Information System (INIS)

    Hong, G. W.; Kim, C. J.; Lee, H. G.; Lee, H. J.; Kim, K. B.; Won, D. Y.; Jang, K. I.; Kwon, S. C.; Kim, W. J.; Ji, Y. A.; Yang, S. W.; Kim, W. K.; Park, S. D.; Lee, M. H.; Lee, D. M.; Park, H. W.; Yu, J. K.; Lee, I. S.; Kim, J. J.; Choi, H. S.; Chu, Y.; Kim, Y. S.; Kim, D. H.

    1997-09-01

    Fabrication of high Tc bulk superconductor and its application, fabrication of superconducting wire for electric power device and analysis for cryogenic system were carried out for developing superconductor application technologies for electric power system. High quality YBaCuO bulk superconductor was fabricated by controlling initial powder preparation process and prototype flywheel energy storage device was designed basically. The superconducting levitation force measuring device was made to examine the property of prepared superconductor specimen. Systematic studies onthe method of starting powder preparation, mechanical fabrication process, heat treatment condition and analysis of plastic deformation were carried out to increase the stability and reproducibility of superconducting wire. A starting power with good reactivity and fine particle size was obtained by mechanical grinding, control of phase assemblage, and emulsion drying method. Ag/BSCCO tape with good cross sectional shape and Jc of 20,000 A/cm 2 was fabricated by applying CIP packing procedure. Multifilamentary wire with Jc of 10,000 A/cm 2 was fabricated by rolling method using square billet as starting shape. The joining of the multifilamentary wire was done by etching and pressing process and showed 50% of joining efficiency. Analysis on the heat loss in cryostat for high Tc superconducting device was carried out for optimum design of the future cryogenic system. (author). 66 refs., 104 figs

  3. AND TECHNOLOGY FOR NATIONAL DEVELOPMENT

    African Journals Online (AJOL)

    within the interest and capabilities of all humans regardless of race, gender, national- ity or ethnicity (Byant & Swinton, ... and technology among men and women that are taken for granted have changed. The marginalization of .... Secondly, science and technology are perceived as powerful model for understanding and ...

  4. Success factors in technology development

    Science.gov (United States)

    Preston, John T.

    1995-01-01

    Universities in the U.S. have a significant impact on business through the transfer of technology. This paper describes goals and philosophy of the Technology Licensing Office at the Massachusetts Institute of Technology. This paper also relates the critical factors for susscessful technology transfer, particularly relating to new business formation. These critical factors include the quality of the technology, the quality of the management, the quality of the investor, the passion for success, and the image of the company. Descriptions of three different levels of investment are also given and the most successful level of investment for starting a new company is reviewed. Licensing to large companies is also briefly reviewed, as this type of licensing requires some different strategies than that of licensing to start-up companies. High quality critical factors and intelligent investment create rewards for the parties and successful ventures.

  5. Development of borehole sealing technology

    International Nuclear Information System (INIS)

    Shimada, Kuniaki; Abe, Kenichi; Tanada, Masuakira; Fujii, Mitsuru

    2004-03-01

    As a part of the geoscientific research in JNC Tono Geoscience Center, we are conducting the borehole investigation as a method of surveying techniques to gain an understanding of geological environment characterization (geology/geological structure, rock hydraulic characteristics, ground water geochemical characteristics and rock mechanics) from surface to deep underground. The borehole for the borehole investigation is used for monitoring hole after the borehole investigation. Since the borehole may act as a passage of groundwater flow and disturb the geological environment artificially, it has to be sealed in finally. Moreover, the hydraulic testing and the geochemical analysis of groundwater that be conducted in the zones injected some kind of lost circulation materials might be impacted on the accuracy of test result. The actual technologies regarding to these themes was researched and evaluated. In the second step, clarification of problems and procedure of R and D for solution of these problems was examined. In order to estimate the effect of lost circulation materials on hydraulic testing, a laboratory test of borehole behavior was performed using a scale model that consisted of a borehole and a water-loss zone. In this test, we found out that the lost circulation material was desorbed from the water-loss zone by back-flow action. It was proved by the test that there is little influence from lost circulation materials on hydraulic testing. Investigation regarding borehole sealing technology was conducted in literature search and interview to overseas researchers. In consequent, three kinds of materials - bentonite clay, bentonite pellet, and ethanol bentonite, were selected as effective sealing material. Moreover, five kinds of methods were selected as effective sealing methods. In water permeability test of sealing material, three kinds of sealing materials indicated lower permeability - order of 10 -11 m/sec, and it was evaluated that it could be worked

  6. FY-95 technology catalog. Technology development for buried waste remediation

    International Nuclear Information System (INIS)

    1995-01-01

    The US Department of Energy's (DOE) Buried Waste Integrated Demonstration (BWID) program, which is now part of the Landfill Stabilization Focus Area (LSFA), supports applied research, development, demonstration, and evaluation of a multitude of advanced technologies dealing with underground radioactive and hazardous waste remediation. These innovative technologies are being developed as part of integrated comprehensive remediation systems for the effective and efficient remediation of buried waste sites throughout the DOE complex. These efforts are identified and coordinated in support of Environmental Restoration (EM-40) and Waste Management (EM-30) needs and objectives. Sponsored by the DOE Office of Technology Development (EM-50), BWID and LSFA work with universities and private industry to develop technologies that are being transferred to the private sector for use nationally and internationally. This report contains the details of the purpose, logic, and methodology used to develop and demonstrate DOE buried waste remediation technologies. It also provides a catalog of technologies and capabilities with development status for potential users. Past FY-92 through FY-94 technology testing, field trials, and demonstrations are summarized. Continuing and new FY-95 technology demonstrations also are described

  7. A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2.

    Science.gov (United States)

    Lieveld, M; Carregosa, A; Benoy, I; Redzic, N; Berth, M; Vanden Broeck, D

    2017-10-01

    Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2. The aim of this study was to develop a high resolution melting (HRM) technology-based assay for sensitive HSV-1 and HSV-2 detection and genotyping. Using a panel of 46 clinical specimens, the performance of the HRM assay was compared to two commercial HSV tests: the HRM assay detected HSV in all 23 positive samples, with no false positive results (100% concordance with HSV I/II Real-TM assay). Additionally, the HRM assay correctly genotyped both HSV types in a subset of these clinical samples, as determined by the Realstar HSV PCR Kit. The HSV HRM assay provides a cost-effective alternative method to conventional more expensive assays and can be used in routine clinical specimens, in cases where it is particularly necessary to detect and distinguish HSV-1 from -2. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Status of Irradiation technology development in JMTR

    International Nuclear Information System (INIS)

    Inaba, Y.; Inoue, S.; Izumo, H.; Kitagishi, S.; Tsuchiya, K.; Saito, T.; Ishitsuka, E.

    2008-01-01

    Irradiation Engineering Section of the Neutron Irradiation and Testing Reactor Center was organized to development the new irradiation technology for the application at JMTR re operation. The new irradiation engineering building was remodeled from the old RI development building, and was started to use from the end of September, 2008. Advanced in situ instrumentation technology (high temperature multi paired thermocouple, ceramic sensor, application of optical measurement), 99M o production technology by new Mo solution irradiation method, recycling technology on used beryllium reflector, and so on are planned as the development of new irradiation technologies. The development will be also important for the education and training programs through the development of young generation in not only Japan but also Asian counties. In this report, as the status of the development the new irradiation technology, new irradiation engineering building, high temperature multi paired thermocouple, experiences of optical measurement, recycling technology on used beryllium reflector are introduced

  9. Policy issues inherent in advanced technology development

    International Nuclear Information System (INIS)

    Baumann, P.D.

    1994-01-01

    In the development of advanced technologies, there are several forces which are involved in the success of the development of those technologies. In the overall development of new technologies, a sufficient number of these forces must be present and working in order to have a successful opportunity at developing, introducing and integrating into the marketplace a new technology. This paper discusses some of these forces and how they enter into the equation for success in advanced technology research, development, demonstration, commercialization and deployment. This paper limits itself to programs which are generally governmental funded, which in essence represent most of the technology development efforts that provide defense, energy and environmental technological products. Along with the identification of these forces are some suggestions as to how changes may be brought about to better ensure success in a long term to attempt to minimize time and financial losses

  10. Status of irradiation technology development in JMTR

    International Nuclear Information System (INIS)

    Inaba, Y.; Inoue, S.; Izumo, H.; Kitagishi, S.; Tsuchiya, K.; Saito, T.; Ishitsuka, E.

    2008-01-01

    Irradiation Engineering Section of the Neutron Irradiation and Testing Reactor Centre was organised to development the new irradiation technology for the application at JMTR re-operation. The new irradiation engineering building was remoulded from the old RI development building, and was started to use from the end of September, 2008. Advanced in-situ instrumentation technology(high temperature multi-paired thermocouple, ceramic sensor,application of optical measurement), 99 Mo production technology by new Mo solution irradiation method,recycling technology on used beryllium reflector, and so on are planned as the development of new irradiation technologies. The development will be also important for the education and training programs through the development of young generation in not only Japan but also Asian countries. In this report, as the status of the development the new irradiation technology, new irradiation engineering building, high temperature multi-paired thermocouple, experiences of optical measurement, recycling technology on used beryllium reflector are introduced

  11. Mobile Sensor Technologies Being Developed

    Science.gov (United States)

    Greer, Lawrence C.; Oberle, Lawrence G.

    2003-01-01

    The NASA Glenn Research Center is developing small mobile platforms for sensor placement, as well as methods for communicating between roving platforms and a central command location. The first part of this project is to use commercially available equipment to miniaturize an existing sensor platform. We developed a five-circuit-board suite, with an average board size of 1.5 by 3 cm. Shown in the preceding photograph, this suite provides all motor control, direction finding, and communications capabilities for a 27- by 21- by 40-mm prototype mobile platform. The second part of the project is to provide communications between mobile platforms, and also between multiple platforms and a central command location. This is accomplished with a low-power network labeled "SPAN," Sensor Platform Area Network, a local area network made up of proximity elements. In practice, these proximity elements are composed of fixed- and mobile-sensor-laden science packages that communicate to each other via radiofrequency links. Data in the network will be shared by a central command location that will pass information into and out of the network through its access to a backbone element. The result will be a protocol portable to general purpose microcontrollers satisfying a host of sensor networking tasks. This network will enter the gap somewhere between television remotes and Bluetooth but, unlike 802.15.4, will not specify a physical layer, thus allowing for many data rates over optical, acoustical, radiofrequency, hardwire, or other media. Since the protocol will exist as portable C-code, developers may be able to embed it in a host of microcontrollers from commercial to space grade and, of course, to design it into ASICs. Unlike in 802.15.4, the nodes will relate to each other as peers. A demonstration of this protocol using the two test bed platforms was recently held. Two NASA modified, commercially available, mobile platforms communicated and shared data with each other and a

  12. Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

    OpenAIRE

    Toriyama, Koji; Suzuki, Takashi; Inoue, Tomoyuki; Eguchi, Hiroshi; Hoshi, Saichi; Inoue, Yoshitsugu; Aizawa, Hideki; Miyoshi, Kazutomi; Ohkubo, Michio; Hiwatashi, Eiji; Tachibana, Hiroshi; Ohashi, Yuichi

    2014-01-01

    We developed an immunochromatographic assay kit that uses fluorescent silica nanoparticles bound to anti-Acanthamoeba antibodies (fluorescent immunochromatographic assay [FICGA]) and evaluated its efficacy for the detection of Acanthamoeba and diagnosis of Acanthamoeba keratitis (AK). The sensitivity of the FICGA kit was evaluated using samples of Acanthamoeba trophozoites and cysts diluted to various concentrations. A conventional immunochromatographic assay kit with latex labels (LICGA) was...

  13. Scoping study to expedite development of a field deployable and portable instrument for UF6 enrichment assay

    OpenAIRE

    Chan, CYG; Valentine, JD; Russo, RE

    2017-01-01

    The primary objective of the present study is to identity the most promising, viable technologies that are likely to culminate in an expedited development of the next-generation, field-deployable instrument for providing rapid, accurate, and precise enrichment assay of uranium hexafluoride (UF6). UF6 is typically involved, and is arguably the most important uranium compound, in uranium enrichment processes. As the first line of defense against proliferation, accurate analytical techniques t...

  14. Electric Aircraft Systems Technology Development

    Data.gov (United States)

    National Aeronautics and Space Administration — This project looks at multiple manned/unmanned full-scale/sub-scale flying research prototypes that will lead to the integration of electric propulsion technology on...

  15. Additive Manufacturing Technology Development Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The 3D Printing In Zero-G (3D Print) technology demonstration project is a proof-of-concept test designed to assess the properties of melt deposition modeling...

  16. Development of Pollution Prevention Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Polle, Juergen [Brooklyn College, The City University of New York (CUNY), Brooklyn, New York, (United States); Sanchez-Delgado, Roberto [Brooklyn College, The City University of New York (CUNY), Brooklyn, New York, (United States)

    2013-12-30

    This project investigated technologies that may reduce environmental pollution. This was a basic research/educational project addressing two major areas: A. In the algae research project, newly isolated strains of microalgae were investigated for feedstock production to address the production of renewable fuels. An existing collection of microalgae was screened for lipid composition to determine strains with superior composition of biofuel molecules. As many microalgae store triacylglycerides in so-called oil bodies, selected candidate strains identified from the first screen that accumulate oil bodies were selected for further biochemical analysis, because almost nothing was known about the biochemistry of these oil bodies. Understanding sequestration of triacylglycerides in intracellular storage compartments is essential to developing better strains for achieving high oil productivities by microalgae. At the onset of the project there was almost no information available on how to obtain detailed profiles of lipids from strains of microalgae. Our research developed analytical methods to determine the lipid profiles of novel microalgal strains. The project was embedded into other ongoing microalgal projects in the Polle laboratory. The project benefited the public, because students were trained in cell cultivation and in the operation of state-of-the-art analytical equipment. In addition, students at Brooklyn College were introduced into the concept of a systems biology approach to study algal biofuels production. B. A series of new nanostructured catalysts were synthesized, and characterized by a variety of physical and chemical methods. Our catalyst design leads to active nanostructures comprising small metal particles in intimate contact with strongly basic sites provided by the supports, which include poly(4-vinylpyridine), magnesium oxide, functionalized multi-walled carbon nanotubes, and graphene oxide. The new materials display a good potential as catalysts

  17. Development of a multi-product leached protein A assay for bioprocess samples containing recombinant human monoclonal antibodies.

    Science.gov (United States)

    Ren, Diya; Darlucio, Maria R; Chou, Judy H

    2011-03-07

    The detection of low level of protein A leached from monoclonal antibody downstream purification process is often interfered by the presence of excess amount of product antibody. In order to prevent this interference, we developed a new multi-product leached protein A assay that used acidification to completely dissociate the IgG-ProteinA complex, followed by neutralization under selected condition to prevent re-formation of the IgG-ProteinA complex. The amount of protein A was then determined by a sandwich immunoassay using Meso Scale Discovery technology. The assay takes approximately 3h to complete for one 96-well plate of samples, and this has been successfully applied to samples containing different monoclonal antibody products examined so far. The data demonstrates that this assay is robust and efficient in determining leached protein A contamination during purification of recombinant monoclonal antibodies. Copyright © 2010 Elsevier B.V. All rights reserved.

  18. Climate change 2 - Canadian technology development conference

    International Nuclear Information System (INIS)

    Tsang, K.T.

    2001-01-01

    These Conference Proceedings summarise the program of the 'Climate Change 2: Canadian Technology Development' Conference held on October 3-5, 2001 in Toronto, Ontario, Canada. The Conference program was multidisciplinary, representing diverse science and technology-oriented associations and organizations from across Canada. The conference was designed to provide a forum to explore and showcase Canadian technologies that could be developed and deployed to reduce greenhouse-gas emissions and/or remove greenhouse gases from the atmosphere. The Conference brought together national and provincial policy makers and innovators to discover, discuss and develop technologies and strategies to encourage appropriate energy, agriculture and forestry technology

  19. Inflammatory markers in Huntington's disease plasma—A robust nanoLC–MRM-MS assay development

    Directory of Open Access Journals (Sweden)

    Melinda Rezeli

    2014-06-01

    Full Text Available The development of an MRM assay for the measurements of six inflammatory markers is presented. We report a robust and sensitive quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing. The assay has a dynamic range with 4 orders of magnitude and the LOQs are in the attomolar range. We used plasma from Huntington's disease gene carriers and healthy controls to compare our MRM method with antibody based methods. Importantly, we found a good agreement between assays for the measurement of C-reactive protein, in contrast to complement component 3 and complement factor H.

  20. CROSSCUTTING TECHNOLOGY DEVELOPMENT AT THE CENTER FOR ADVANCED SEPARATION TECHNOLOGIES

    Energy Technology Data Exchange (ETDEWEB)

    Christopher E. Hull

    2006-05-15

    This Technical Progress Report describes progress made on the twenty nine subprojects awarded in the second year of Cooperative Agreement DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices.

  1. Crosscutting Technology Development at the Center for Advanced Separation Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Christopher E. Hull

    2006-09-30

    This Technical Progress Report describes progress made on the twenty nine subprojects awarded in the second year of Cooperative Agreement DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices.

  2. CROSSCUTTING TECHNOLOGY DEVELOPMENT AT THE CENTER FOR ADVANCED SEPARATION TECHNOLOGIES

    Energy Technology Data Exchange (ETDEWEB)

    Christopher E. Hull

    2005-11-04

    This Technical Progress Report describes progress made on the twenty nine subprojects awarded in the second year of Cooperative Agreement DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices.

  3. Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species.

    Science.gov (United States)

    Silbereisen, Angelika; Tamborrini, Marco; Wittwer, Matthias; Schürch, Nadia; Pluschke, Gerd

    2015-10-05

    Brucella, a Gram-negative bacterium, is classified as a potential bioterrorism agent mainly due to the low dose needed to cause infection and the ability to transmit the bacteria via aerosols. Goats/sheep, cattle, pigs, dogs, sheep and rodents are infected by B. melitensis, B. abortus, B. suis, B. canis, B. ovis and B. neotomae, respectively, the six classical Brucella species. Most human cases are caused by B. melitensis and B. abortus. Our aim was to specifically detect Brucellae with 'smooth' lipopolysaccharide (LPS) using a highly sensitive monoclonal antibody (mAb) based immunological assay. To complement molecular detection systems for potential bioterror agents, as required by international biodefense regulations, sets of mAbs were generated by B cell hybridoma technology and used to develop immunological assays. The combination of mAbs most suitable for an antigen capture assay format was identified and an immunoassay using the Luminex xMAP technology was developed. MAbs specific for the LPS O-antigen of Brucella spp. were generated by immunising mice with inactivated B. melitensis or B. abortus cells. Most mAbs recognised both B. melitensis and B. abortus and antigen binding was not impeded by inactivation of the bacterial cells by γ irradiation, formalin or heat treatment, a step required to analyse the samples immunologically under biosafety level two conditions. The Luminex assay recognised all tested Brucella species with 'smooth' LPS with detection limits of 2×10(2) to 8×10(4) cells per mL, depending on the species tested. Milk samples spiked with Brucella spp. cells were identified successfully using the Luminex assay. In addition, the bead-based immunoassay was integrated into a multiplex format, allowing for simultaneous, rapid and specific detection of Brucella spp., Bacillus anthracis, Francisella tularensis and Yersinia pestis within a single sample. Overall, the robust Luminex assay should allow detection of Brucella spp. in both natural

  4. Co-ordinated research program on development of kits for radioimmunometric assays of tumor markers

    International Nuclear Information System (INIS)

    Acevedo Castro, B.E.; Perera Negrin, Y.; Pichardo Diaz, D.; Murugiah, R.; Ayala Avila, M.; Gavilondo Cowley, J.; Ruiz Pena, M.; Caso Pena, R.; Hernandez Pagarizabal, M.

    1999-01-01

    Mice were immunized with semen and natural PSA, following three different schemes. Splenocytes from two hyper immune animals were fused with the P3/x63.Ag8.653 myeloma under conventional hybridoma procedures. Stable hybrid cell cultures secreting antibodies specific to natural PSA were obtained by cloning dilutions procedures. With a group of stable hybridoma cultures we developed epitope characterization assays to determine whether the antibodies were capable of recognizing PSA. According to the recognition level in ELISA, the hybridomas were classified in different groups,. We select the best pairs of Mabs for developing a total and free PSA assays based on ELISA or IRMA format. Our total-PSA based on IRMA format presented a good correlation in comparison with CIS bio total PSA assay. We recommend our anti-PSA monoclonal antibodies to develop an IRMA assay for total PSA. Cuban free-PSA assay is under evaluation at present. (author)

  5. Development of an immunochromatographic assay for the ß-adrenergic agonist feed additive zilpaterol

    Science.gov (United States)

    A zilpaterol immunochromatographic assay was developed as an economical and user friendly rapid detection method for zilpaterol. The assay sensitivity was 1.7 – 23.2 ng/g or mL with a variety of feed and animal matrices and did not cross react with clenbuterol or ractopamine. No sample pre-treatme...

  6. The development of a high-content screening binding assay for the smoothened receptor.

    Science.gov (United States)

    Bee, Weilin Tiger; Xie, Wensheng; Truong, Maggie; Will, Matthew; Turunen, Brandon; Zuercher, William J; McMillan, Lynette; Li, Hu; Hornberger, Keith R; Davenport, Elizabeth A; Ames, Robert S; Kallal, Lorena A

    2012-08-01

    In this study, the development of an image-based high-content screening (HCS) binding assay for the seven-transmembrane (7TM) receptor Smoothened (Smo) is described. Using BacMam-based gene delivery of Smo, BODIPY-cyclopamine as a fluorescent probe, and a confocal imaging system, a robust 384-well assay that could be used for high-throughput compound profiling activities was developed. The statistically robust HCS binding assay was developed through optimization of multiple parameters, including cell transduction conditions, Smo expression levels, the image analysis algorithm, and staining procedures. Evaluation of structurally diverse compounds, including functional Smo activators, inhibitors, and related analogs, demonstrated good compound potency correlations between high-content imaging binding, membrane fluorescence polarization binding, and gene reporter assays. Statistical analysis of data from a screening test set of compounds at a single 10-µM concentration suggested that the high-content imaging Smo binding assay is amenable for use in hit identification. The 384-well HCS assay was rapidly developed and met statistical assay performance targets, thus demonstrating its utility as a fluorescent whole-cell binding assay suitable for compound screening and profiling.

  7. Development of a thyroperoxidase inhibition assay for high-throughput screening

    Science.gov (United States)

    High-throughput screening (HTPS) assays to detect inhibitors of thyroperoxidase (TPO), the enzymatic catalyst for thyroid hormone (TH) synthesis, are not currently available. Herein we describe the development of a HTPS TPO inhibition assay. Rat thyroid microsomes and a fluores...

  8. Information technology for sustainable development

    DEFF Research Database (Denmark)

    Holgaard, Jette Egelund; Guerra, Aida; Knoche, Hendrik

    2013-01-01

    In this paper we present different strategies to integrate concerns about sustainability into Information and Communication Technology (ITC) projects by use of problem based learning (PBL) methodology. In alignment with PBL we introduce two different models for problem analysis where students move...... that initiate design, implementation and test of ICT for SD. On the empirical level we draw from experiments in autumn 2009 and spring 2013 within the field of Media technology. Observations, text analysis of students’ work as well as reflections from staff has been relied on in order to analyse the practical...

  9. History of nuclear technology development in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Yamashita, Kiyonobu, E-mail: yamashita.kiyonobu@jaea.go.jp [Visiting Professor, at the Faculty of Petroleum and Renewable Energy Engineering, University Teknologi Malaysia Johor Bahru 81310 (Malaysia); General Advisor Nuclear HRD Centre, Japan Atomic Energy Agency, TOKAI-mura, NAKA-gun, IBARAKI-ken, 319-1195 (Japan)

    2015-04-29

    Nuclear technology development in Japan has been carried out based on the Atomic Energy Basic Act brought into effect in 1955. The nuclear technology development is limited to peaceful purposes and made in a principle to assure their safety. Now, the technologies for research reactors radiation application and nuclear power plants are delivered to developing countries. First of all, safety measures of nuclear power plants (NPPs) will be enhanced based on lesson learned from TEPCO Fukushima Daiichi NPS accident.

  10. History of nuclear technology development in Japan

    International Nuclear Information System (INIS)

    Yamashita, Kiyonobu

    2015-01-01

    Nuclear technology development in Japan has been carried out based on the Atomic Energy Basic Act brought into effect in 1955. The nuclear technology development is limited to peaceful purposes and made in a principle to assure their safety. Now, the technologies for research reactors radiation application and nuclear power plants are delivered to developing countries. First of all, safety measures of nuclear power plants (NPPs) will be enhanced based on lesson learned from TEPCO Fukushima Daiichi NPS accident

  11. Development of NDT technology of the welds

    International Nuclear Information System (INIS)

    Li Jianwen; Xu Yansen; Wang Zengyong

    2002-01-01

    Non-destructive testing (NDT) and its up-to-date development are summarized, including the advantages and disadvantages and the development of NDT technology. The up-to-date development of X-ray imaging and industrial CT is emphasised on, and the fundamental theory of ultrasonic imaging and related signal processing technology is introduced

  12. Understanding Technology: Key to Development. | Bvekerwa ...

    African Journals Online (AJOL)

    This paper observes that technology as a concept is not fully understood in the developing world. More often than not technology and science are taken to mean the same thing. It is argued here that the two terms are not synonymous but are actually two sides of the same coin. It is further illustrated that technology is not ...

  13. The develop of technology production in Spain

    International Nuclear Information System (INIS)

    Fernandez Labastida, J. M.

    2007-01-01

    Spanish Science and Technology system has been very effective in scientific production but not in technology transfer to economic activities. A cultural change is needed to improve the knowledge transfer mechanisms. Some specific actions are proposed in order to develop useful instruments to achieve a better technology transfer system. (Author)

  14. Pathways to Inclusive Development through Innovation, Technology ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Technology and innovation can drive economic growth, help solve social and environmental problems, and reduce poverty. All countries need to develop the capacity to produce and use science and technology themselves and adapt knowledge to their needs and contexts. While industrial upgrading through technology ...

  15. Pipe Leak Detection Technology Development

    Science.gov (United States)

    The U. S. Environmental Protection Agency (EPA) has determined that one of the nation’s biggest infrastructural needs is the replacement or rehabilitation of the water distribution and transmission systems. The institution of more effective pipe leak detection technology will im...

  16. Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus.

    Science.gov (United States)

    Wanzhe, Y; Jianuan, L; Peng, L; Jiguo, S; Ligong, C; Juxiang, L

    2016-01-01

    Duck tembusu virus (DTMUV) has caused significant economic losses to the poultry industry in China since the spring of 2010. In this study, a nano-PCR assay targeting E gene of DTMUV was developed and their sensitivities and specificities were investigated. Under the optimized conditions of nano-PCR assay for detection of DTMUV, the nano-PCR assay was 10-fold more sensitive than a conventional PCR assay. The lower detection limit of the nano-PCR assay was 1·8 × 10(2)  copies μl(-1) of DTMUV RNA, as no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nano-PCR assay for the detection of DTMUV. The sensitive, and specific nano-PCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of DTMUV-infection. A nanoparticle-assisted polymerase chain reaction (nano-PCR) assay was developed in this study for the rapid detection of duck tembusu virus (DTMUV) with high sensitivity and specificity. This technique has potential application in both clinical diagnosis and field surveillance of DTMUV-infection. © 2015 The Society for Applied Microbiology.

  17. Clean Technology Evaluation & Workforce Development Program

    Energy Technology Data Exchange (ETDEWEB)

    Patricia Glaza

    2012-12-01

    The overall objective of the Clean Technology Evaluation portion of the award was to design a process to speed up the identification of new clean energy technologies and match organizations to testing and early adoption partners. The project was successful in identifying new technologies targeted to utilities and utility technology integrators, in developing a process to review and rank the new technologies, and in facilitating new partnerships for technology testing and adoption. The purpose of the Workforce Development portion of the award was to create an education outreach program for middle & high-school students focused on clean technology science and engineering. While originally targeting San Diego, California and Cambridge, Massachusetts, the scope of the program was expanded to include a major clean technology speaking series and expo as part of the USA Science & Engineering Festival on the National Mall in Washington, D.C.

  18. Lunar Surface Systems Supportability Technology Development Roadmap

    Science.gov (United States)

    Oeftering, Richard C.; Struk, Peter M.; Green, Jennifer L.; Chau, Savio N.; Curell, Philip C.; Dempsey, Cathy A.; Patterson, Linda P.; Robbins, William; Steele, Michael A.; DAnnunzio, Anthony; hide

    2011-01-01

    The Lunar Surface Systems Supportability Technology Development Roadmap is a guide for developing the technologies needed to enable the supportable, sustainable, and affordable exploration of the Moon and other destinations beyond Earth. Supportability is defined in terms of space maintenance, repair, and related logistics. This report considers the supportability lessons learned from NASA and the Department of Defense. Lunar Outpost supportability needs are summarized, and a supportability technology strategy is established to make the transition from high logistics dependence to logistics independence. This strategy will enable flight crews to act effectively to respond to problems and exploit opportunities in an environment of extreme resource scarcity and isolation. The supportability roadmap defines the general technology selection criteria. Technologies are organized into three categories: diagnostics, test, and verification; maintenance and repair; and scavenge and recycle. Furthermore, "embedded technologies" and "process technologies" are used to designate distinct technology types with different development cycles. The roadmap examines the current technology readiness level and lays out a four-phase incremental development schedule with selection decision gates. The supportability technology roadmap is intended to develop technologies with the widest possible capability and utility while minimizing the impact on crew time and training and remaining within the time and cost constraints of the program.

  19. Innovative Technology Development Program. Final summary report

    International Nuclear Information System (INIS)

    Beller, J.

    1995-08-01

    Through the Office of Technology Development (OTD), the U.S. Department of Energy (DOE) has initiated a national applied research, development, demonstration, testing, and evaluation program, whose goal has been to resolve the major technical issues and rapidly advance technologies for environmental restoration and waste management. The Innovative Technology Development (ITD) Program was established as a part of the DOE, Research, Development, Demonstration, Testing, and Evaluation (RDDT ampersand E) Program. The plan is part of the DOE's program to restore sites impacted by weapons production and to upgrade future waste management operations. On July 10, 1990, DOE issued a Program Research and Development Announcement (PRDA) through the Idaho Operations Office to solicit private sector help in developing innovative technologies to support DOE's clean-up goals. This report presents summaries of each of the seven projects, which developed and tested the technologies proposed by the seven private contractors selected through the PRDA process

  20. Innovative Technology Development Program. Final summary report

    Energy Technology Data Exchange (ETDEWEB)

    Beller, J.

    1995-08-01

    Through the Office of Technology Development (OTD), the U.S. Department of Energy (DOE) has initiated a national applied research, development, demonstration, testing, and evaluation program, whose goal has been to resolve the major technical issues and rapidly advance technologies for environmental restoration and waste management. The Innovative Technology Development (ITD) Program was established as a part of the DOE, Research, Development, Demonstration, Testing, and Evaluation (RDDT&E) Program. The plan is part of the DOE`s program to restore sites impacted by weapons production and to upgrade future waste management operations. On July 10, 1990, DOE issued a Program Research and Development Announcement (PRDA) through the Idaho Operations Office to solicit private sector help in developing innovative technologies to support DOE`s clean-up goals. This report presents summaries of each of the seven projects, which developed and tested the technologies proposed by the seven private contractors selected through the PRDA process.

  1. Radioactive Dry Process Material Treatment Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Park, J. J.; Hung, I. H.; Kim, K. K. (and others)

    2007-06-15

    The project 'Radioactive Dry Process Material Treatment Technology Development' aims to be normal operation for the experiments at DUPIC fuel development facility (DFDF) and safe operation of the facility through the technology developments such as remote operation, maintenance and pair of the facility, treatment of various high level process wastes and trapping of volatile process gases. DUPIC Fuel Development Facility (DFDF) can accommodate highly active nuclear materials, and now it is for fabrication of the oxide fuel by dry process characterizing the proliferation resistance. During the second stage from march 2005 to February 2007, we carried out technology development of the remote maintenance and the DFDF's safe operation, development of treatment technology for process off-gas, and development of treatment technology for PWR cladding hull and the results was described in this report.

  2. PubChem BioAssay: A Decade's Development toward Open High-Throughput Screening Data Sharing.

    Science.gov (United States)

    Wang, Yanli; Cheng, Tiejun; Bryant, Stephen H

    2017-07-01

    High-throughput screening (HTS) is now routinely conducted for drug discovery by both pharmaceutical companies and screening centers at academic institutions and universities. Rapid advance in assay development, robot automation, and computer technology has led to the generation of terabytes of data in screening laboratories. Despite the technology development toward HTS productivity, fewer efforts were devoted to HTS data integration and sharing. As a result, the huge amount of HTS data was rarely made available to the public. To fill this gap, the PubChem BioAssay database ( https://www.ncbi.nlm.nih.gov/pcassay/ ) was set up in 2004 to provide open access to the screening results tested on chemicals and RNAi reagents. With more than 10 years' development and contributions from the community, PubChem has now become the largest public repository for chemical structures and biological data, which provides an information platform to worldwide researchers supporting drug development, medicinal chemistry study, and chemical biology research. This work presents a review of the HTS data content in the PubChem BioAssay database and the progress of data deposition to stimulate knowledge discovery and data sharing. It also provides a description of the database's data standard and basic utilities facilitating information access and use for new users.

  3. Technology transfer in the Clean Development Mechanism

    International Nuclear Information System (INIS)

    De Coninck, H.C.; Haake, F.; Van der Linden, N.H.

    2007-01-01

    Technology transfer is often mentioned as an ancillary benefit of the Kyoto Protocol's Clean Development Mechanism (CDM), but this claim has never been researched or substantiated. The question of technology transfer is important from two perspectives: for host countries, whether the CDM provides a corridor for foreign, climate-friendly technologies and investment, and for industrialised countries as it provides export potential for climate-friendly technologies developed as a consequence of stringent greenhouse gas targets. In order to better understand whether technology transfer from the EU and elsewhere is occurring through the CDM, and what is the value of the associated foreign investment, this paper examines technology transfer in the 63 CDM projects that were registered on January 1st, 2006. Technology originates from outside the host country in almost 50% of the evaluated projects. In the projects in which the technology originates from outside the host country, 80% use technology from the European Union. Technologies used in non-CO2 greenhouse gas and wind energy projects, and a substantial share of the hydropower projects, use technology from outside the host country, but biogas, agricultural and biomass projects mainly use local technology. The associated investment value with the CDM projects that transferred technology is estimated to be around 470 million Euros, with about 390 coming from the EU. As the non-CO2 greenhouse gas projects had very low capital costs, the investment value was mostly in the more capital-intensive wind energy and hydropower projects

  4. Learning in renewable energy technology development

    International Nuclear Information System (INIS)

    Junginger, M.

    2005-01-01

    The main objectives of this thesis are: to investigate technological change and cost reduction for a number of renewable electricity technologies by means of the experience curve approach; to address related methodological issues in the experience curve approach, and, based on these insights; and to analyze the implications for achieving the Dutch renewable electricity targets for the year 2020 within a European context. In order to meet these objectives, a number of research questions have been formulated: What are the most promising renewable electricity technologies for the Netherlands until 2020 under different technological, economic and environmental conditions?; To what extent is the current use of the experience curve approach to investigate renewable energy technology development sound, what are differences in the utilization of this approach and what are possible pitfalls?; How can the experience curve approach be used to describe the potential development of partially new energy technologies, such as offshore wind energy? Is it possible to describe biomass fuel supply chains with experience curves? What are the possibilities and limits of the experience curve approach when describing non-modular technologies such as large (biomass) energy plants?; What are the main learning mechanisms behind the cost reduction of the investigated technologies?; and How can differences in the technological progress of renewable electricity options influence the market diffusion of renewable electricity technologies, and what implications can varying technological development and policy have on the implementation of renewable electricity technologies in the Netherlands? The development of different renewable energy technologies is investigated by means of some case studies. The possible effects of varying technological development in combination with different policy backgrounds are illustrated for the Netherlands. The thesis focuses mainly on the development of investment

  5. Microhole Drilling Tractor Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Western Well Tool

    2007-07-09

    In an effort to increase the U.S. energy reserves and lower costs for finding and retrieving oil, the USDOE created a solicitation to encourage industry to focus on means to operate in small diameter well-Microhole. Partially in response to this solicitation and because Western Well Tool's (WWT) corporate objective to develop small diameter coiled tubing drilling tractor, WWT responded to and was awarded a contract to design, prototype, shop test, and field demonstrate a Microhole Drilling Tractor (MDT). The benefit to the oil industry and the US consumer from the project is that with the MDT's ability to facilitate Coiled Tubing drilled wells to be 1000-3000 feet longer horizontally, US brown fields can be more efficiently exploited resulting in fewer wells, less environmental impact, greater and faster oil recovery, and lower drilling costs. Shortly after award of the contract, WWT was approached by a major oil company that strongly indicated that the specified size of a tractor of 3.0 inches diameter was inappropriate and that immediate applications for a 3.38-inch diameter tractor would substantially increase the usefulness of the tool to the oil industry. Based on this along with an understanding with the oil company to use the tractor in multiple field applications, WWT applied for and was granted a no-cost change-of-scope contract amendment to design, manufacture, assemble, shop test and field demonstrate a prototype a 3.38 inch diameter MDT. Utilizing existing WWT tractor technology and conforming to an industry developed specification for the tool, the Microhole Drilling Tractor was designed. Specific features of the MDT that increase it usefulness are: (1) Operation on differential pressure of the drilling fluid, (2) On-Off Capability, (3) Patented unique gripping elements (4) High strength and flexibility, (5) Compatibility to existing Coiled Tubing drilling equipment and operations. The ability to power the MDT with drilling fluid results in a

  6. Development of a Waste Vault Directional Assay System (VDAS)

    Energy Technology Data Exchange (ETDEWEB)

    LaFleur, Adrienne Marie [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-11-08

    We are developing a portable neutron and gamma detector called VDAS with sufficient sensitivity and directional capability such that it could operate in high gamma dose rate environments. This detector will be designed such that it can be remotely deployed and adaptable to a broad range of possible facility infrastructure to record a 3D array of gross neutron and gamma measurements, including axial and radial rotation, for identifying potential SNM material locations.

  7. The first FDA marketing authorizations of next-generation sequencing technology and tests: challenges, solutions and impact for future assays.

    Science.gov (United States)

    Bijwaard, Karen; Dickey, Jennifer S; Kelm, Kellie; Težak, Živana

    2015-01-01

    The rapid emergence and clinical translation of novel high-throughput sequencing technologies created a need to clarify the regulatory pathway for the evaluation and authorization of these unique technologies. Recently, the US FDA authorized for marketing four next generation sequencing (NGS)-based diagnostic devices which consisted of two heritable disease-specific assays, library preparation reagents and a NGS platform that are intended for human germline targeted sequencing from whole blood. These first authorizations can serve as a case study in how different types of NGS-based technology are reviewed by the FDA. In this manuscript we describe challenges associated with the evaluation of these novel technologies and provide an overview of what was reviewed. Besides making validated NGS-based devices available for in vitro diagnostic use, these first authorizations create a regulatory path for similar future instruments and assays.

  8. KSC Education Technology Research and Development Plan

    Science.gov (United States)

    Odell, Michael R. L.

    2003-01-01

    Educational technology is facilitating new approaches to teaching and learning science, technology, engineering, and mathematics (STEM) education. Cognitive research is beginning to inform educators about how students learn providing a basis for design of more effective learning environments incorporating technology. At the same time, access to computers, the Internet and other technology tools are becoming common features in K-20 classrooms. Encouraged by these developments, STEM educators are transforming traditional STEM education into active learning environments that hold the promise of enhancing learning. This document illustrates the use of technology in STEM education today, identifies possible areas of development, links this development to the NASA Strategic Plan, and makes recommendations for the Kennedy Space Center (KSC) Education Office for consideration in the research, development, and design of new educational technologies and applications.

  9. Technology development for radiation shielding analysis

    International Nuclear Information System (INIS)

    Ha, Jung Woo; Lee, Jae Kee; Kim, Jong Kyung

    1986-12-01

    Radiation shielding analysis in nuclear engineering fields is an important technology which is needed for the calculation of reactor shielding as well as radiation related safety problems in nuclear facilities. Moreover, the design technology required in high level radioactive waste management and disposal facilities is faced on serious problems with rapidly glowing nuclear industry development, and more advanced technology has to be developed for tomorrow. The main purpose of this study is therefore to build up the self supporting ability of technology development for the radiation shielding analysis in order to achieve successive development of nuclear industry. It is concluded that basic shielding calculations are possible to handle and analyze by using our current technology, but more advanced technology is still needed and has to be learned for the degree of accuracy in two-dimensional shielding calculation. (Author)

  10. Advanced Reactor Technology -- Regulatory Technology Development Plan (RTDP)

    International Nuclear Information System (INIS)

    Moe, Wayne Leland

    2015-01-01

    This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a ''critical path'' for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain ''minimum'' levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial ''first step'' in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by

  11. Advanced Reactor Technologies - Regulatory Technology Development Plan (RTDP)

    Energy Technology Data Exchange (ETDEWEB)

    Moe, Wayne L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2017-08-23

    This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a “critical path” for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain “minimum” levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial “first step” in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by technology development studies, the anticipated regulatory

  12. Advanced Reactor Technology -- Regulatory Technology Development Plan (RTDP)

    Energy Technology Data Exchange (ETDEWEB)

    Moe, Wayne Leland [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-05-01

    This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a “critical path” for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain “minimum” levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial “first step” in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by technology development studies, the anticipated regulatory

  13. Genesis and development of DPPH method of antioxidant assay.

    Science.gov (United States)

    Kedare, Sagar B; Singh, R P

    2011-08-01

    α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

  14. Technological Developments in Networking, Education and Automation

    CERN Document Server

    Elleithy, Khaled; Iskander, Magued; Kapila, Vikram; Karim, Mohammad A; Mahmood, Ausif

    2010-01-01

    "Technological Developments in Networking, Education and Automation" includes a set of rigorously reviewed world-class manuscripts addressing and detailing state-of-the-art research projects in the following areas: Computer Networks: Access Technologies, Medium Access Control, Network architectures and Equipment, Optical Networks and Switching, Telecommunication Technology, and Ultra Wideband Communications. Engineering Education and Online Learning: including development of courses and systems for engineering, technical and liberal studies programs; online laboratories; intelligent

  15. Enrichment Assay Methods Development for the Integrated Cylinder Verification System

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Leon E.; Misner, Alex C.; Hatchell, Brian K.; Curtis, Michael M.

    2009-10-22

    International Atomic Energy Agency (IAEA) inspectors currently perform periodic inspections at uranium enrichment plants to verify UF6 cylinder enrichment declarations. Measurements are typically performed with handheld high-resolution sensors on a sampling of cylinders taken to be representative of the facility's entire product-cylinder inventory. Pacific Northwest National Laboratory (PNNL) is developing a concept to automate the verification of enrichment plant cylinders to enable 100 percent product-cylinder verification and potentially, mass-balance calculations on the facility as a whole (by also measuring feed and tails cylinders). The Integrated Cylinder Verification System (ICVS) could be located at key measurement points to positively identify each cylinder, measure its mass and enrichment, store the collected data in a secure database, and maintain continuity of knowledge on measured cylinders until IAEA inspector arrival. The three main objectives of this FY09 project are summarized here and described in more detail in the report: (1) Develop a preliminary design for a prototype NDA system, (2) Refine PNNL's MCNP models of the NDA system, and (3) Procure and test key pulse-processing components. Progress against these tasks to date, and next steps, are discussed.

  16. SRS environmental technology development field test platform

    International Nuclear Information System (INIS)

    Riha, B.D.; Rossabi, J.; Eddy-Dilek, C.A.

    1995-01-01

    A critical and difficult step in the development and implementation of new technologies for environmental monitoring and characterization is successfully transferring these technologies to industry and government users for routine assessment and compliance activities. The Environmental Sciences Section of the DOE Savannah River Technology Center provides a forum for developers, potential users, and regulatory organizations to evaluate new technologies in comparison with baseline technologies in a well characterized field test bed. The principal objective of this project is to conduct comprehensive, objective field tests of monitoring and characterization technologies that are not currently used in EPA standard methods and evaluate their performance during actual operating conditions against baseline methods. This paper provides an overview of the field test site and a description of some of the technologies demonstrated at the site including their field applications

  17. The development of a state-of-the-art assay system for uranium-235 in solutions

    Energy Technology Data Exchange (ETDEWEB)

    Parker, J.L.; Sampson, T.E.; Cowder, L.R.; Kern, E.A.; Garcia, D.L.; Ensslin, N.

    1986-12-01

    We describe the development of a high-accuracy, high-precision, and high-throughput system for the assay of /sup 235/U in solution samples. The tradeoffs involved in the various development steps are discussed and the ultimate system performance is documented. Assay accuracy and precision better than 0.2% should be attained in routine use. This is a significant improvement in the state of the art.

  18. The development of a state-of-the-art assay system for uranium-235 in solutions

    International Nuclear Information System (INIS)

    Parker, J.L.; Sampson, T.E.; Cowder, L.R.; Kern, E.A.; Garcia, D.L.; Ensslin, N.

    1986-12-01

    We describe the development of a high-accuracy, high-precision, and high-throughput system for the assay of 235 U in solution samples. The tradeoffs involved in the various development steps are discussed and the ultimate system performance is documented. Assay accuracy and precision better than 0.2% should be attained in routine use. This is a significant improvement in the state of the art

  19. Development of real-time PCR assay for genetic identification of the mottled skate, Beringraja pulchra.

    Science.gov (United States)

    Hwang, In Kwan; Lee, Hae Young; Kim, Min-Hee; Jo, Hyun-Su; Choi, Dong-Ho; Kang, Pil-Won; Lee, Yang-Han; Cho, Nam-Soo; Park, Ki-Won; Chae, Ho Zoon

    2015-10-01

    The mottled skate, Beringraja pulchra is one of the commercially important fishes in the market today. However, B. pulchra identification methods have not been well developed. The current study reports a novel real-time PCR method based on TaqMan technology developed for the genetic identification of B. pulchra. The mitochondrial cytochrome oxidase subunit 1 (COI) nucleotide sequences of 29 B. pulchra, 157 skates and rays reported in GenBank DNA database were comparatively analyzed and the COI sequences specific to B. pulchra was identified. Based on this information, a system of specific primers and Minor Groove Binding (MGB) TaqMan probe were designed. The assay successfully discriminated in 29 specimens of B. pulchra and 27 commercial samples with unknown species identity. For B. pulchra DNA, an average Threshold Cycle (Ct) value of 19.1±0.1 was obtained. Among 27 commercial samples, two samples showed average Ct values 19.1±0.0 and 26.7±0.1, respectively and were confirmed to be B. pulchra based on sequencing. The other samples tested showed undetectable or extremely weak signals for the target fragment, which was also consistent with the sequencing results. These results reveal that the method developed is a rapid and efficient tool to identify B. pulchra and might prevent fraud or mislabeling during the distribution of B. pulchra products. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  20. Social and Technological Development in Context

    DEFF Research Database (Denmark)

    Koch, Christian

    1997-01-01

    This papers studies the processes developing technology and its social "sorroundings", the social networks. Positions in the debate on technological change is discussed. A central topic is the enterprise external development and decision processes and their interplay with the enterprise internal...

  1. Information and Communication Technologies for Development in ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Information and Communication Technologies for Development in the Middle East : Exploration. This project seeks to foster an inclusive knowledge society by building capacity to apply information and communication technology (ICT) to human, social and economic development in the Middle ... Royal Scientific Society.

  2. Aligning Technology Education Teaching with Brain Development

    Science.gov (United States)

    Katsioloudis, Petros

    2015-01-01

    This exploratory study was designed to determine if there is a level of alignment between technology education curriculum and theories of intellectual development. The researcher compared Epstein's Brain Growth Theory and Piaget's Status of Intellectual Development with technology education curriculum from Australia, England, and the United…

  3. New nuclear technology; International developments. Review 1995

    International Nuclear Information System (INIS)

    Devell, L.; Aggeryd, I.; Hultgren, Aa.; Lundell, B.; Pedersen, T.

    1995-09-01

    A summary review of the development of new nuclear rector technology is presented in this report. Fuel cycle strategies and waste handling developments are also commented. Different plans for dismantling nuclear weapons are presented. 18 refs

  4. Development of Food Preservation and Processing Technologies by Radiation Technology

    International Nuclear Information System (INIS)

    Byun, Myung Woo; Lee, Ju Won; Kim, Jae Hun

    2007-07-01

    To secure national food resources, development of energy-saving food processing and preservation technologies, establishment of method on improvement of national health and safety by development of alternative techniques of chemicals and foundation of the production of hygienic food and public health related products by irradiation technology were studied. Results at current stage are following: As the first cooperative venture business technically invested by National Atomic Research Development Project, institute/company's [technology-invested technology foundation No. 1] cooperative venture, Sun-BioTech Ltd., was founded and stated its business. This suggested new model for commercialization and industrialization of the research product by nation-found institute. From the notice of newly approved product list about irradiated food, radiation health related legal approval on 7 food items was achieved from the Ministry of health and wellfare, the Korea Food and Drug Administration, and this contributed the foundation of enlargement of practical use of irradiated food. As one of the foundation project for activation of radiation application technology for the sanitation and secure preservation of special food, such as military meal service, food service for patient, and food for sports, and instant food, such as ready-to-eat/ready-to-cook food, the proposal for radiation application to the major military commander at the Ministry of National Defence and the Joint Chiefs of Staff was accepted for the direction of military supply development in mid-termed plan for the development of war supply. Especially, through the preliminary research and the development of foundation technology for the development of the Korean style space food and functional space food, space Kimch with very long shelf life was finally developed. The development of new item/products for food and life science by combining RT/BT, the development of technology for the elimination/reduction of

  5. Development of Food Preservation and Processing Technologies by Radiation Technology

    Energy Technology Data Exchange (ETDEWEB)

    Byun, Myung Woo; Lee, Ju Won; Kim, Jae Hun (and others)

    2007-07-15

    To secure national food resources, development of energy-saving food processing and preservation technologies, establishment of method on improvement of national health and safety by development of alternative techniques of chemicals and foundation of the production of hygienic food and public health related products by irradiation technology were studied. Results at current stage are following: As the first cooperative venture business technically invested by National Atomic Research Development Project, institute/company's [technology-invested technology foundation No. 1] cooperative venture, Sun-BioTech Ltd., was founded and stated its business. This suggested new model for commercialization and industrialization of the research product by nation-found institute. From the notice of newly approved product list about irradiated food, radiation health related legal approval on 7 food items was achieved from the Ministry of health and wellfare, the Korea Food and Drug Administration, and this contributed the foundation of enlargement of practical use of irradiated food. As one of the foundation project for activation of radiation application technology for the sanitation and secure preservation of special food, such as military meal service, food service for patient, and food for sports, and instant food, such as ready-to-eat/ready-to-cook food, the proposal for radiation application to the major military commander at the Ministry of National Defence and the Joint Chiefs of Staff was accepted for the direction of military supply development in mid-termed plan for the development of war supply. Especially, through the preliminary research and the development of foundation technology for the development of the Korean style space food and functional space food, space Kimch with very long shelf life was finally developed. The development of new item/products for food and life science by combining RT/BT, the development of technology for the elimination/reduction of

  6. Forward-Looking Planning of Technology Development

    Directory of Open Access Journals (Sweden)

    Katarzyna Halicka

    2015-12-01

    Full Text Available The main aim of this article is to adapt the Future-Oriented Technology Analysis (FTA to prospective planning of technology development. Firstly, the article presents the assumptions, methods and idea, as well as the concept of the FTA method. Moreover, selected publications on the use of this method were analysed. Then, an original, base model of forward-looking planning of technology development was constructed and presented. The end result of this process will be the development of the localized in time, presented in graphic form, action plan referred to as the route of technology development. Basing on the literature review and the research projects a preliminary route of development of arbitrarily chosen technology was also built and presented.

  7. Development of environmental radiation control technology

    International Nuclear Information System (INIS)

    Kim, Ingyu; Kim, Enhan; Keum, Dongkwon

    2012-04-01

    To develop the comprehensive environmental radiation management technology, - An urban atmospheric dispersion model and decision-aiding model have been developed. - The technologies for assessing the radiation impact to non-human biota and the environmental medium contamination have developed. - The analytical techniques of the indicator radionuclides related to decommissioning of nuclear facilities and nuclear waste repository have been developed. - The national environmental radiation impact has been assessed, and the optimum management system of natural radiation has been established

  8. Development of high burnup nuclear fuel technology

    International Nuclear Information System (INIS)

    Suk, Ho Chun; Kang, Young Hwan; Jung, Jin Gone; Hwang, Won; Park, Zoo Hwan; Ryu, Woo Seog; Kim, Bong Goo; Kim, Il Gone

    1987-04-01

    The objectives of the project are mainly to develope both design and manufacturing technologies for 600 MWe-CANDU-PHWR-type high burnup nuclear fuel, and secondly to build up the foundation of PWR high burnup nuclear fuel technology on the basis of KAERI technology localized upon the standard 600 MWe-CANDU- PHWR nuclear fuel. So, as in the first stage, the goal of the program in the last one year was set up mainly to establish the concept of the nuclear fuel pellet design and manufacturing. The economic incentives for high burnup nuclear fuel technology development are improvement of fuel utilization, backend costs plant operation, etc. Forming the most important incentives of fuel cycle costs reduction and improvement of power operation, etc., the development of high burnup nuclear fuel technology and also the research on the incore fuel management and safety and technologies are necessary in this country

  9. Development of technologies for solar energy utilization

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    With relation to the development of photovoltaic power systems for practical use, studies were made on thin-substrate polycrystalline solar cells and thin-film solar cells as manufacturing technology for solar cells for practical use. The technological development for super-high efficiency solar cells was also being advanced. Besides, the research and development have been conducted of evaluation technology for photovoltaic power systems and systems to utilize the photovoltaic power generation and peripheral technologies. The demonstrative research on photovoltaic power systems was continued. The international cooperative research on photovoltaic power systems was also made. The development of a manufacturing system for compound semiconductors for solar cells was carried out. As to the development of solar energy system technologies for industrial use, a study of elemental technologies was first made, and next the development of an advanced heat process type solar energy system was commenced. In addition, the research on passive solar systems was made. An investigational study was carried out of technologies for solar cities and solar energy snow melting systems. As international joint projects, studies were made of solar heat timber/cacao drying plants, etc. The paper also commented on projects for international cooperation for the technological development of solar energy utilization systems. 26 figs., 15 tabs.

  10. Critical stages of a biodetection platform development from sensor chip fabrication to surface chemistry and assay development

    Science.gov (United States)

    Uludag, Yildiz

    2014-06-01

    Once viewed solely as a tool to analyse biomolecular interactions, biosensors are gaining widespread interest for diagnostics, biological defense, environmental and quality assurance in agriculture/food industries. Advanced micro fabrication techniques have facilitated integration of microfluidics with sensing functionalities on the same chip making system automation more convenient1. Biosensor devices relying on lab-on-a-chip technologies and nanotechnology has attracted much of attention in recent years for biological defense research and development. However, compared with the numerous publications and patents available, the commercialization of biosensors technology has significantly lagged behind the research output. This paper reviews the reasons behind the slow commercialisation of biosensors with an insight to the critical stages of a biosensor development from the sensor chip fabrication to surface chemistry applications and nanotechnology applications in sensing with case studies. In addition, the paper includes the description of a new biodetection platform based on Real-time Electrochemical ProfilingTM (REPTM) that comprises novel electrode arrays and nanoparticle based sensing. The performance of the REPTM platform has been tested for the detection of Planktothrix agardhii, one of the toxic bloom-forming cyanobacteria, usually found in shallow fresh water sources that can be used for human consumption. The optimised REPTM assay allowed the detection of P. agardhii DNA down to 6 pM. This study, showed the potential of REPTM as a new biodetection platform for toxic bacteria and hence further studies will involve the development of a portable multi-analyte biosensor based on REPTM technology for on-site testing.

  11. Technology development and applications at Fernald

    International Nuclear Information System (INIS)

    Pettit, P.J.; Skriba, M.C.; Warner, R.D.

    1995-01-01

    At the Fernald Environmental Management Project (FEMP) northwest of Cincinnati, Ohio, the U.S. Department of Energy and contractor Fernald Environmental Restoration Management Corporation (FERMCO) are aggressively pursuing both the development and the application of improved, innovative technology to the environmental restoration task. Application of emerging technologies is particularly challenging in a regulatory environment that places pressure on operational managers to develop and meet tight schedules. The regulatory and operational needs make close communication essential between technology developers and technology users (CERCLA/RCRA Unit managers). At Fernald this cooperation and communication has led, not only to the development and demonstration of new technologies with applications at other sites, but also to application of new technologies directly to the Fernald clean up. New technologies have been applied to improve environmental safety and health, improve the effectiveness of restoration efforts, and to cut restoration costs. The paper will describe successful efforts to develop and apply new technologies at the FEMP and will emphasize those technologies that have been applied and are planned for use in the clean up of this former uranium production facility

  12. Mirror fusion vacuum technology developments

    International Nuclear Information System (INIS)

    Batzer, T.H.; Call, W.R.

    1983-01-01

    Magnetic Mirror Fusion experiments, such as MFTF-B+T (Mirror Fusion Test Facility-B, Tritium Upgrade) and foreseeable follow-on devices, have operational and maintenance requirements that have not yet been fully demonstrated. Among those associated with vacuum technology are the very-high continuous-pumping speeds, 10 7 to 10 8 l/s for D 2 , T 2 and, to a lesser extent, He; the early detection of water leaks from the very-high heat-flux neutral-beam dumps and the detection and location of leaks in the superconducting magnets not protected by guard vacuums. Possible solutions to these problems have been identified and considerable progress has been made toward successfully demonstrating their feasibility

  13. Technologies for a sustainable development; Technologies pour un developpement durable

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    The European Event on Technology (EET), a recurrent annual event since 1992, is a major meeting opportunity for researchers and engineers as well as private and public decision-makers, on technologies, their evolution and their industrial and social implications. In less than a decade, sustainable development has become both an economic and a political priority. It was urgent and legitimate that those who are the mainsprings should take hold of the subject and give it technological content, estimate its costs and define clear timetables. The debates consist of: plenary sessions on environmental, social and economic stakes of sustainable development and the challenges for, and commitment of engineers, managers and politicians with respect to these goals; and workshops, which provide an overview of recently acquired or upcoming technologies developed by sector: energy, transports, new information technologies, new industrial manufacturing technologies (materials, products, services), waste management, global environment monitoring, water management, bio-technologies, and innovation management. This document brings together the different talks given by the participants. Among these, the following ones fall into the energy and environment scope: energy efficiency of buildings: towards energy autonomy; superconductors enable in new millennium for electric power industry; advanced gas micro-turbine-driven generator technology; environmental and technical challenges of an offshore wind farm; future nuclear energy systems; modelling combustion in engines: progress and prospects for reducing emissions; on-board computers: reduction in consumption and emissions of engine-transmission units for vehicles; polymer-lithium batteries: perspectives for zero-emission traction; hybrid vehicles and energy/environmental optimization: paths and opportunities; fuel cells and zero-emission: perspectives and developments; global change: causes, modeling and economic issues; the GMES

  14. Development of isotope hydrology technology in China

    International Nuclear Information System (INIS)

    Li Zhangsu

    1988-01-01

    The development of isotope hydrology technology in China is described. The isotope technology provides an independent approach for solving hydrological problems. Isotope hydrology is applied in three ways: the use of change in environmental isotopic composition of water (especially used in water resources exploitation), the use of artificial radioactive tracers and the use of redioisotope instruments. Many important achievements have been obtained in application of isotopic hydrology technology. For the sake of promoting rapid development of isotope hydrology the topics on management, technology and others are commented

  15. Space Technology Mission Directorate: Game Changing Development

    Science.gov (United States)

    Gaddis, Stephen W.

    2015-01-01

    NASA and the aerospace community have deep roots in manufacturing technology and innovation. Through it's Game Changing Development Program and the Advanced Manufacturing Technology Project NASA develops and matures innovative, low-cost manufacturing processes and products. Launch vehicle propulsion systems are a particular area of interest since they typically comprise a large percentage of the total vehicle cost and development schedule. NASA is currently working to develop and utilize emerging technologies such as additive manufacturing (i.e. 3D printing) and computational materials and processing tools that could dramatically improve affordability, capability, and reduce schedule for rocket propulsion hardware.

  16. Development of coal energy utilization technologies

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    Coal liquefaction produces new and clean energy by performing hydrogenation, decomposition and liquefaction on coal under high temperatures and pressures. NEDO has been developing bituminous coal liquefaction technologies by using a 150-t/d pilot plant. It has also developed quality improving and utilization technologies for liquefied coal, whose practical use is expected. For developing coal gasification technologies, construction is in progress for a 200-t/d pilot plant for spouted bed gasification power generation. NEDO intends to develop coal gasification composite cycle power generation with high efficiency and of environment harmonious type. This paper summarizes the results obtained during fiscal 1994. It also dwells on technologies to manufacture hydrogen from coal. It further describes development of technologies to manufacture methane and substituting natural gas (SNG) by hydrogenating and gasifying coal. The ARCH process can select three operation modes depending on which of SNG yield, thermal efficiency or BTX yield is targeted. With respect to promotion of coal utilization technologies, description is given on surveys on development of next generation technologies for coal utilization, and clean coal technology promotion projects. International coal utilization and application projects are also described. 9 figs., 3 tabs.

  17. Development of a paper-based vertical flow SERS assay for citrulline detection using aptamer-conjugated gold nanoparticles

    Science.gov (United States)

    Locke, Andrea; Deutz, Nicolaas; Coté, Gerard

    2018-02-01

    Research toward development of point-of-care (POC) technologies is emerging as a means for diagnosis and monitoring of patients outside the hospital. These POC devices typically utilize assays capable of detecting low level biomarkers indicative of specific diseases. L-citrulline, an α-amino acid produced in the intestinal mucosa cells, is one such biomarker typically found circulating within the plasma at physiological concentrations of 40 μM. Researchers have found that intestinal enterocyte malfunction causes its level to be significantly lowered, establishing it as a potential diagnostic biomarker for gut function. Our research group has proposed the development of a surface enhanced Raman spectroscopy (SERS) based assay, using vertical flow paper fluidics, for citrulline detection. The assay consists of a fluorescently active, Raman reporter labeled aptamer conjugated on gold nanoparticles. The aptamer changes its confirmation on binding to its target, which in turn changes the distance between the Raman active molecule and the nanoparticle surface. These particles were embedded within a portable chip consisting of cellulose-based paper. After the chips were loaded with different concentrations of free L-citrulline in phosphate buffer, time was given for the assay to interact with the sample. A handheld Raman spectrometer (638 nm; Ocean Optics) was used to measure the SERS intensity. Results showed decrease in intensity with increasing concentration of L-citrulline (0-50μM).

  18. Development of microLIPS (Luciferase Immunoprecipitation Systems): a novel microfluidic assay for rapid serum antibody detection

    Science.gov (United States)

    Chandrangsu, Matt; Burbelo, Peter D.; Iadarola, Michael J.; Smith, Paul D.; Morgan, Nicole Y.

    2012-06-01

    There is considerable interest in the development of rapid, point-of-care antibody detection for the diagnosis of infectious and auto-immune diseases. In this paper, we present work on the development of a self-contained microfluidic format for the Luciferase Immunoprecipitation Systems (LIPS) assay. Whereas the majority of immunoassays for antigen-specific antibodies employ either bacteria- or yeast-expressed proteins and require the use of secondary antibodies, the LIPS technique uses a fusion protein comprised of a Renilla luciferase reporter and the antigen of interest produced via mammalian cell culture, ensuring the addition of mammalian post-translational modifications. Patient serum is mixed with the fusion protein and passed over immobilized Protein A/G; after washing, the only remaining luciferase-tagged antigens are those retained by specific antibodies. These can be quantitatively measured using chemiluminescence upon the introduction of coelenterazine. The assay has been successfully employed for a wide variety of diseases in a microwell format. We report on a recent demonstration of rapid HSV-2 diagnosis with the LIPS assay in a microfluidic format, using one microliter of serum and obtaining results in under ten minutes. We will also discuss recent progress on two fronts, both aimed at the deployment of this technology in the field: first, simplifying assay operation through the automation of flow control using power-free means; and second, efforts to increase signal levels, primarily through strategies to increase antibody binding capacity, in order to move towards portable battery powered electronics.

  19. A Comparison of Assay Performance Between the Calcium Mobilization and the Dynamic Mass Redistribution Technologies for the Human Urotensin Receptor

    Science.gov (United States)

    Lee, Mi Young; Mun, Jihye; Lee, Jeong Hyun; Lee, Sunghou; Lee, Byung Ho

    2014-01-01

    Abstract The popular screening method for urotensin (UT) receptor antagonists is to measure the intracellular calcium concentration with a calcium-sensitive fluorescent dye. This assay format has an inherent limitation on the problem related to the fluorescence interference as it involves fluorescent dyes. In the present study, a label-free assay for the screening of UT receptor antagonists was developed by using dynamic mass redistribution (DMR) assay based on label-free optical biosensor. The addition of urotensin II (UII) stimulated a DMR profile to HEK293 cells stably expressing the human UT receptor (HEK293UT cells) but not on parental cells. The EC50 value of UII in label-free assay was 4.58 nM, which is very similar to that in conventional calcium mobilization assay (4.15 nM). Compared with the calcium mobilization assay for UII (Z′ factor, 0.77), the current label-free assay presented improved Z′ factor (0.81), with a relatively similar S/B ratio (28.0 and 25.6, respectively). The known high-affinity UT receptor antagonists, SB657510, GSK562590, and urantide, exhibited comparable IC50 values but rather less potent in the DMR assay than in calcium mobilization. Our DMR assay was able to present various functional responses, including inverse agonism in SB657510 and GSK1562590 as well as partial agonism in urantide. Moreover, the DMR assay exerted the stable antagonist window upon the minimal agonist stimulus. These results suggest that the label-free cell-based UT receptor assay can be applicable to evaluate the various functional activities of UT receptor-related drug candidates. PMID:25147908

  20. Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species.

    Science.gov (United States)

    Chae, Hansong; Han, Seung Jung; Kim, Su-Young; Ki, Chang-Seok; Huh, Hee Jae; Yong, Dongeun; Koh, Won-Jung; Shin, Sung Jae

    2017-09-01

    The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of this assay were to (i) discriminate between the Mycobacterium tuberculosis complex (MTBC) and NTM using rv0577 or RD750, (ii) differentiate M. tuberculosis ( M. tuberculosis ) from MTBC using RD9, (iii) selectively identify the widespread M. tuberculosis Beijing genotype by targeting mtbk_20680 , and (iv) simultaneously detect five clinically important NTM ( M. avium , M. intracellulare , M. abscessus , M. massiliense , and M. kansasii ) by targeting IS 1311 , DT1, mass_3210 , and mkan_rs12360 An initial evaluation of the multiplex PCR assay using reference strains demonstrated 100% specificity for the targeted Mycobacterium species. Analytical sensitivity ranged from 1 to 10 pg for extracted DNA and was 10 3 and 10 4 CFU for pure cultures and nonhomogenized artificial sputum cultures, respectively, of the targeted species. The accuracy of the multiplex PCR assay was further evaluated using 55 reference strains and 94 mycobacterial clinical isolates. Spoligotyping, multilocus sequence analysis, and a commercial real-time PCR assay were employed as standard assays to evaluate the multiplex PCR assay with clinical M. tuberculosis and NTM isolates. The PCR assay displayed 100% identification agreement with the standard assays. Our multiplex PCR assay is a simple, convenient, and reliable technique for differential identification of MTBC, M. tuberculosis , M. tuberculosis Beijing genotype, and major NTM species. Copyright © 2017 American Society for Microbiology.

  1. Technological Microbiology: Development and Applications

    Directory of Open Access Journals (Sweden)

    Luciana C. Vitorino

    2017-05-01

    Full Text Available Over thousands of years, modernization could be predicted for the use of microorganisms in the production of foods and beverages. However, the current accelerated pace of new food production is due to the rapid incorporation of biotechnological techniques that allow the rapid identification of new molecules and microorganisms or even the genetic improvement of known species. At no other time in history have microorganisms been so present in areas such as agriculture and medicine, except as recognized villains. Currently, however, beneficial microorganisms such as plant growth promoters and phytopathogen controllers are required by various agricultural crops, and many species are being used as biofactories of important pharmacological molecules. The use of biofactories does not end there: microorganisms have been explored for the synthesis of diverse chemicals, fuel molecules, and industrial polymers, and strains environmentally important due to their biodecomposing or biosorption capacity have gained interest in research laboratories and in industrial activities. We call this new microbiology Technological Microbiology, and we believe that complex techniques, such as heterologous expression and metabolic engineering, can be increasingly incorporated into this applied science, allowing the generation of new and improved products and services.

  2. Technological Microbiology: Development and Applications.

    Science.gov (United States)

    Vitorino, Luciana C; Bessa, Layara A

    2017-01-01

    Over thousands of years, modernization could be predicted for the use of microorganisms in the production of foods and beverages. However, the current accelerated pace of new food production is due to the rapid incorporation of biotechnological techniques that allow the rapid identification of new molecules and microorganisms or even the genetic improvement of known species. At no other time in history have microorganisms been so present in areas such as agriculture and medicine, except as recognized villains. Currently, however, beneficial microorganisms such as plant growth promoters and phytopathogen controllers are required by various agricultural crops, and many species are being used as biofactories of important pharmacological molecules. The use of biofactories does not end there: microorganisms have been explored for the synthesis of diverse chemicals, fuel molecules, and industrial polymers, and strains environmentally important due to their biodecomposing or biosorption capacity have gained interest in research laboratories and in industrial activities. We call this new microbiology Technological Microbiology, and we believe that complex techniques, such as heterologous expression and metabolic engineering, can be increasingly incorporated into this applied science, allowing the generation of new and improved products and services.

  3. Technological Microbiology: Development and Applications

    Science.gov (United States)

    Vitorino, Luciana C.; Bessa, Layara A.

    2017-01-01

    Over thousands of years, modernization could be predicted for the use of microorganisms in the production of foods and beverages. However, the current accelerated pace of new food production is due to the rapid incorporation of biotechnological techniques that allow the rapid identification of new molecules and microorganisms or even the genetic improvement of known species. At no other time in history have microorganisms been so present in areas such as agriculture and medicine, except as recognized villains. Currently, however, beneficial microorganisms such as plant growth promoters and phytopathogen controllers are required by various agricultural crops, and many species are being used as biofactories of important pharmacological molecules. The use of biofactories does not end there: microorganisms have been explored for the synthesis of diverse chemicals, fuel molecules, and industrial polymers, and strains environmentally important due to their biodecomposing or biosorption capacity have gained interest in research laboratories and in industrial activities. We call this new microbiology Technological Microbiology, and we believe that complex techniques, such as heterologous expression and metabolic engineering, can be increasingly incorporated into this applied science, allowing the generation of new and improved products and services. PMID:28539920

  4. Information and Communication Technology for Rural Development

    OpenAIRE

    Ankur Mani Tripathi; Abhishek Kumar Singh; Arvind Kumar

    2012-01-01

    Due to the lack of knowledge and use of ICT in rural areas, development is at a very low rate. Some improvement and advancement in the technologies provided by the government but there is no more effect in the development of rural areas. Information and communication technologies are developing day by day but are lessapplicable in rural areas. Lack of communication and resources are the cause of undeveloped. Main problem are in rural areas are electricity, communication, transportation and la...

  5. Nuclear technology for sustainable development

    International Nuclear Information System (INIS)

    2001-01-01

    Introduces three of the IAEA's current programmes: Promoting food security - use of the sterile insect technique to eradicate the tsetse fly in Sub-Saharan Africa; Managing water resources - use of isotope hydrology to check water for traces of arsenic in Bangladesh; Improving human health - use of nuclear techniques for diagnosis, imaging and cancer treatment in developing countries

  6. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

    OpenAIRE

    Sam R. Nugen; Catherine Fill; Yuhong Wang

    2012-01-01

    Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, th...

  7. Development of silicon photonic microring resonator biosensors for multiplexed cytokine assays and in vitro diagnostics

    Science.gov (United States)

    Luchansky, Matthew Sam

    In order to guide critical care therapies that are personalized to a patient's unique disease state, a diagnostic or theranostic medical device must quickly provide a detailed biomolecular understanding of disease onset and progression. This detailed molecular understanding of cellular processes and pathways requires the ability to measure multiple analytes in parallel. Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. Currently used analytical methods are deficient in that they often provide either highly quantitative data for a single biomarker or qualitative data for many targets, but methods that simultaneously provide highly quantitative analysis of many targets have yet to be adequately developed. Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. In an effort to fill this unmet need, this doctoral dissertation describes the development of a clinically translational biosensing technology based on silicon photonics and developed in the chemistry research laboratory of Ryan C. Bailey. Silicon photonic microring resonators, a class of high-Q optical sensors, represent a promising platform for rapid, multiparameter in vitro measurements. The original device design utilizes 32-ring arrays for real-time biomolecular sensing without fluorescent labels, and these optical biosensors display great potential for more highly multiplexed (100s-1000s) measurements based on the impressive scalability of silicon device fabrication. Though this technology can be used to detect a variety of

  8. Solar cell materials developing technologies

    CERN Document Server

    Conibeer, Gavin J

    2014-01-01

    This book presents a comparison of solar cell materials, including both new materials based on organics, nanostructures and novel inorganics and developments in more traditional photovoltaic materials. It surveys the materials and materials trends in the field including third generation solar cells (multiple energy level cells, thermal approaches and the modification of the solar spectrum) with an eye firmly on low costs, energy efficiency and the use of abundant non-toxic materials.

  9. Development of Nuclear Analytical Technology

    Energy Technology Data Exchange (ETDEWEB)

    Park, Yong Joon; Kim, J. Y.; Sohn, S. C. (and others)

    2007-06-15

    The pre-treatment and handling techniques for the micro-particles in swipe samples were developed for the safeguards purpose. The development of screening technique for the swipe samples has been established using the nuclear fission track method as well as the alpha track method. The laser ablation system to take a nuclear particle present in swipe was designed and constructed for the determination of the enrichment factors for uranium or plutonium, and its performance was tested in atmosphere as well as in vacuum. The optimum conditions for the synthesis of silica based micro-particles were obtained for mass production. The optimum ion exchange resin was selected and the optimum conditions for the uranium adsorption in resin bead technique were established for the development of the enrichment factor for nuclear particles in swipe. The established technique was applied to the swipe taken directly from the nuclear facility and also to the archive samples of IAEA's environmental swipes. The evaluation of dose rate of neutron and secondary gamma-ray for the radiation shields were carried out to design the NIPS system, as well as the evaluation of the thermal neutron concentration effect by the various reflectors. D-D neutron generator was introduced as a neutron source for the NIPS system to have more advantages such as easier control and moderation capability than the {sup 252}Cf source. Simulated samples for explosive and chemical warfare were prepared to construct a prompt gamma-ray database. Based on the constructed database, a computer program for the detection of illicit chemical and nuclear materials was developed using the MATLAB software.

  10. Technology development multidimensional review for engineering and technology managers

    CERN Document Server

    Neshati, Ramin; Watt, Russell; Eastham, James

    2014-01-01

    Developing new products, services, systems, and processes has become an imperative for any firm expecting to thrive in today’s fast-paced and hyper-competitive environment.  This volume integrates academic and practical insights to present fresh perspectives on new product development and innovation, showcasing lessons learned on the technological frontier.  The first part emphasizes decision making.  The second part focuses on technology evaluation, including cost-benefit analysis, material selection, and scenarios. The third part features in-depth case studies to present innovation management tools, such as customer needs identification, technology standardization, and risk management. The fourth part highlights important international trends, such as globalization and outsourcing. Finally the fifth part explores social and political aspects.

  11. Integrated Micro Product and Technology Development

    DEFF Research Database (Denmark)

    Hansen, Hans Nørgaard

    2003-01-01

    The paper addresses the issues of integrated micro product and technology development. The implications of the decisions in the design phase on the subsequent manufacturing processes are considered vital. A coherent process chain is a necessary prerequisite for the realisation of the industrial...... potential of micro technology....

  12. Recent developments in chemical decontamination technology

    Energy Technology Data Exchange (ETDEWEB)

    Wood, C.J. [Electric Power Research Institute, Palo Alto, CA (United States)

    1995-03-01

    Chemical decontamination of parts of reactor coolant systems is a mature technology, used routinely in many BWR plants, but less frequently in PWRs. This paper reviews recent developments in the technology - corrosion minimization, waste processing and full system decontamination, including the fuel. Earlier work was described in an extensive review published in 1990.

  13. Readiness for banking technologies in developing countries

    African Journals Online (AJOL)

    Are customers who have a higher perceived desirability of using technological .... low-income people. Hypothesis development. 1Before it is possible to determine the relationship between technology readiness and key demographics ... not possible to draw a random sample of respondents from the target population.

  14. Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus

    Directory of Open Access Journals (Sweden)

    Kanisht Batra

    2015-11-01

    Full Text Available Aim: The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP targeting highly conserved genomic regions of Capripoxvirus (CaPVs and its comparative evaluation with real-time polymerase chain reaction (PCR. Material and Methods: Lyophilized vaccine strain of sheeppox virus (SPPV was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32 coding gene targeting highly conserved genomic regions of CaPV responsible for causing sheep pox, goat pox, and lumpy skin disease in sheep, goat and cattle respectively. Serial tenfold dilution of SPPV recombinant plasmid DNA was used for a calculating limit of detection. Analytical sensitivity and specificity were performed. Results: The test described is quick (30 min, sensitive and specific for detection of CaPVs. The described assay did not show any cross-reactivity to other related viruses that cause apparently similar clinical signs. It was found to be ten times more sensitive than conventional PCR however, 100 times less sensitive than quantitative PCR (qPCR. LAMP assay results were monitored by color change method using picogreen dye and agarose gel electrophoresis. Conclusion: LAMP assay can be a very good alternative for CaPV detection to other molecular techniques requiring sophisticated equipments.

  15. Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

    Directory of Open Access Journals (Sweden)

    Yang Yang

    2017-01-01

    Full Text Available Porcine circovirus virus type II (PCV2 is the etiology of postweaning multisystemic wasting syndrome (PMWS, porcine dermatitis, nephropathy syndrome (PDNS, and necrotizing pneumonia. Rapid diagnosis tool for detection of PCV2 plays an important role in the disease control and eradication program. Recombinase polymerase amplification (RPA assays using a real-time fluorescent detection (PCV2 real-time RPA assay and RPA combined with lateral flow dipstick (PCV2 RPA LFD assay were developed targeting the PCV2 ORF2 gene. The results showed that the sensitivity of the PCV2 real-time RPA assay was 102 copies per reaction within 20 min at 37°C and the PCV2 RPA LFD assay had a detection limit of 102 copies per reaction in less than 20 min at 37°C. Both assays were highly specific for PCV2, with no cross-reactions with porcine circovirus virus type 1, foot-and-mouth disease virus, pseudorabies virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and classical swine fever virus. Therefore, the RPA assays provide a novel alternative for simple, sensitive, and specific identification of PCV2.

  16. CROSSCUTTING TECHNOLOGY DEVELOPMENT AT THE CENTER FOR ADVANCED SEPARATION TECHNOLOGIES

    Energy Technology Data Exchange (ETDEWEB)

    Hugh W. Rimmer

    2004-05-12

    This Technical Progress Report describes progress made on the seventeen subprojects awarded in the first year of Cooperative Agreement DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. This work is summarized in the body of the main report: the individual sub-project Technical Progress Reports are attached as Appendices. Due to the time taken up by the solicitation/selection process, these cover the initial 6-month period of project activity only. The U.S. is the largest producer of mining products in the world. In 1999, U.S. mining operations produced $66.7 billion worth of raw materials that contributed a total of $533 billion to the nation's wealth. Despite these contributions, the mining industry has not been well supported with research and development funds as compared to mining industries in other countries. To overcome this problem, the Center for Advanced Separation Technologies (CAST) was established to develop technologies that can be used by the U.S. mining industry to create new products, reduce production costs, and meet environmental regulations. Originally set up by Virginia Tech and West Virginia University, this endeavor has been expanded into a seven-university consortium--Virginia Tech, West Virginia University, University of Kentucky, University of Utah, Montana Tech, New Mexico Tech and University of Nevada, Reno--that is supported through U.S. DOE Cooperative Agreement No. DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. Much of the research to be conducted with Cooperative Agreement funds will be longer-term, high-risk, basic research and will be carried out in five broad areas: (1) Solid-solid separation (2) Solid-liquid separation (3) Chemical/Biological Extraction (4) Modeling and Control, and (5) Environmental Control.

  17. Energy technology transfer to developing countries

    International Nuclear Information System (INIS)

    Butera, F.; Farinelli, U.

    1992-01-01

    With the use of critical analyses of some examples of technology transfer by industrialized to third world countries, this paper illustrates the importance, in technology transfer, of giving due consideration to the specific social and marketing contexts of the targeted developing country and its physical and financial capability to acquire all the technology necessary to make the total realization of a desired industrial scheme feasible from the economic, technical and social points of view. It also indicates that the most effective transfers are those in which efforts are made to optimize local work force learning levels, process scheme efficiency and cost through the careful integration of innovative with conventional technologies

  18. The Clean Development Mechanism and Technology Transfer

    DEFF Research Database (Denmark)

    Aggarwal, Aradhna

    2017-01-01

    This study assesses the impact of the Clean Development Mechanism (CDM) on the transfer of clean technology in India. The reason this study is unique is because firstly, it adopts an outcome-oriented approach to define ‘technology transfer’, which means that technology transfer occurs if firms...... are able to upgrade their ‘dynamic capabilities’. It uses three indicators of firms’ dynamic capabilities: R&D expenditures to sales ratio, fuel consumption to sales ratio and total factor productivity growth. Secondly, it moves away from the analysis of technology transfer claims made in either Project...

  19. Scoping study to expedite development of a field deployable and portable instrument for UF6 enrichment assay

    Energy Technology Data Exchange (ETDEWEB)

    Chan, George; Valentine, John D.; Russo, Richard E.

    2017-09-14

    The primary objective of the present study is to identity the most promising, viable technologies that are likely to culminate in an expedited development of the next-generation, field-deployable instrument for providing rapid, accurate, and precise enrichment assay of uranium hexafluoride (UF6). UF6 is typically involved, and is arguably the most important uranium compound, in uranium enrichment processes. As the first line of defense against proliferation, accurate analytical techniques to determine the uranium isotopic distribution in UF6 are critical for materials verification, accounting, and safeguards at enrichment plants. As nuclear fuel cycle technology becomes more prevalent around the world, international nuclear safeguards and interest in UF6 enrichment assay has been growing. At present, laboratory-based mass spectrometry (MS), which offers the highest attainable analytical accuracy and precision, is the technique of choice for the analysis of stable and long-lived isotopes. Currently, the International Atomic Energy Agency (IAEA) monitors the production of enriched UF6 at declared facilities by collecting a small amount (between 1 to 10 g) of gaseous UF6 into a sample bottle, which is then shipped under chain of custody to a central laboratory (IAEA’s Nuclear Materials Analysis Laboratory) for high-precision isotopic assay by MS. The logistics are cumbersome and new shipping regulations are making it more difficult to transport UF6. Furthermore, the analysis is costly, and results are not available for some time after sample collection. Hence, the IAEA is challenged to develop effective safeguards approaches at enrichment plants. In-field isotopic analysis of UF6 has the potential to substantially reduce the time, logistics and expense of sample handling. However, current laboratory-based MS techniques require too much infrastructure and operator expertise for field deployment and operation. As outlined in the IAEA Department of Safeguards Long

  20. Mixed Waste Integrated Program emerging technology development

    Energy Technology Data Exchange (ETDEWEB)

    Berry, J.B. [Oak Ridge National Lab., TN (United States); Hart, P.W. [USDOE, Washington, DC (United States)

    1994-06-01

    The US Department of Energy (DOE) is responsible for the management and treatment of its mixed low-level wastes (MLLW). MLLW are regulated under both the Resource Conservation and Recovery Act and various DOE orders. Over the next 5 years, DOE will manage over 1.2 m{sup 3} of MLLW and mixed transuranic (MTRU) wastes. In order to successfully manage and treat these mixed wastes, DOE must adapt and develop characterization, treatment, and disposal technologies which will meet performance criteria, regulatory approvals, and public acceptance. Although technology to treat MLLW is not currently available without modification, DOE is committed to developing such treatment technologies and demonstrating them at the field scale by FY 1997. The Office of Research and Development`s Mixed Waste Integrated Program (MWIP) within the DOE Office of Environmental Management (EM), OfFice of Technology Development, is responsible for the development and demonstration of such technologies for MLLW and MTRU wastes. MWIP advocates and sponsors expedited technology development and demonstrations for the treatment of MLLW.

  1. Development of a solid-phase assay for measurement of proteolytic enzyme activity

    International Nuclear Information System (INIS)

    Varani, J.; Johnson, K.; Kaplan, J.

    1980-01-01

    A solid-phase, plate assay was developed for the measurement of proteolytic enzyme activity. In this assay procedure, radiolabeled substrates were dried onto the surface of microtiter wells. Following drying, the wells were washed two times with saline to remove the nonadherent substrate. When proteolytic enzymes were added to the wells, protein hydrolysis occurred, releasing radioactivity into the supernatant fluid. The amount of protein hydrolysis that occurred was reflected by the amount of radioactivity in the supernatant fluid. When 125 I-hemoglobin was used as the substrate, it was as susceptible to hydrolysis by trypsin in the solid-phase assay as it was in solution in a standard assay procedure. Protease activity from a variety of sources (including from viable cells as well as from extracellular sources) were also able to hydrolyze the hemoglobin on the plate. 125 I-Labeled serum albumen, fibrinogen, and rat pulmonary basement membrane were also susceptible to hydrolysis by trypsin in the solid phase. When [ 14 C]elastin was dried onto the plate, it behaved in a similar manner to elastin in solution. It was resistant to hydrolysis by nonspecific proteases such as trypsin and chymotrypsin but was highly susceptible to hydrolysis by elastase. The solid-phase plate assay has several features which recommended it for routine use. It is as sensitive as standard tube assays (and much more sensitive than routinely used colormetric assays). It is quick and convenient; there are no precipitation, centrifugation, or filtration steps. In addition, very small volumes of radioactive wastes are generated. Another advantage of the solid-phase plate assay is the resistance of the dried substrates to spontaneous breakdown and to microbial contamination. Finally, this assay is suitable for use with viable cells as well as for extracellular proteases

  2. INNOVATIVE DEVELOPMENT OF WAREHOUSE TECHNOLOGY

    Directory of Open Access Journals (Sweden)

    Judit OLÁH

    2017-12-01

    Full Text Available The smooth operation of stocking and the warehouse play a very important role in all manufacturing companies; therefore ongoing monitoring and application of new techniques is essential to increase efficiency. The aim of our research is twofold: the utilization of the pallet shuttle racking system, and the introduction of a development opportunity by the merging of storage and order picking operations in the pallet shuttle system. It can be concluded that it is beneficial for the company to purchase two mobile cars in order to increase the utilization of the pallet shuttle racking system from 60% to 72% and that of the storage from 74% to 76%. We established that after the merging of the storage and order picking activities within the pallet shuttle system, the forklift driver can also complete the selection activities immediately after storage. By merging the two operations and saving time the number of forklift drivers can be reduced from 4 to 3 per shift.

  3. High-Speed Sealift Technology Development Plan

    National Research Council Canada - National Science Library

    2002-01-01

    .... The purpose of the project was to define the technology investments required to enable development of the high-speed commercial and military ships needed to provide realistic future mission capabilities...

  4. Decontamination Technology Development for Nuclear Research Facilities

    International Nuclear Information System (INIS)

    Oh, Won Zin; Jung, Chong Hun; Choi, Wang Kyu; Won, Hui Jun; Kim, Gye Nam

    2004-02-01

    Technology development of surface decontamination in the uranium conversion facility before decommissioning, technology development of component decontamination in the uranium conversion facility after decommissioning, uranium sludge treatment technology development, radioactive waste soil decontamination technology development at the aim of the temporary storage soil of KAERI, Optimum fixation methodology derivation on the soil and uranium waste, and safety assessment methodology development of self disposal of the soil and uranium waste after decontamination have been performed in this study. The unique decontamination technology applicable to the component of the nuclear facility at room temperature was developed. Low concentration chemical decontamination technology which is very powerful so as to decrease the radioactivity of specimen surface under the self disposal level was developed. The component decontamination technology applicable to the nuclear facility after decommissioning by neutral salt electro-polishing was also developed. The volume of the sludge waste could be decreased over 80% by the sludge waste separation method by water. The electrosorption method on selective removal of U(VI) to 1 ppm of unrestricted release level using the uranium-containing lagoon sludge waste was tested and identified. Soil decontamination process and equipment which can reduce the soil volume over 90% were developed. A pilot size of soil decontamination equipment which will be used to development of real scale soil decontamination equipment was designed, fabricated and demonstrated. Optimized fixation methodology on soil and uranium sludge was derived from tests and evaluation of the results. Safety scenario and safety evaluation model were development on soil and uranium sludge aiming at self disposal after decontamination

  5. Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology.

    Science.gov (United States)

    Zhao, Zhuoya; Xing, Zenan; Zhou, Min; Chen, Yi; Li, Chenzhong; Wang, Ruozhong; Xu, Wenzhong; Ma, Mi

    2015-11-01

    DELLA proteins and phytohormone gibberellin act together to control convergence point of plant development. A gibberellin-bound nuclear receptor that interacts with the N-terminal domain of DELLA proteins is required for gibberellin induced degradation of DELLA proteins. N-terminal DELLA domain includes two conserved motifs: DELLA and VHYNP. However, their respective functions remain unclear. Meanwhile, the identification and detection of several bioactive gibberellins from the more than 100 gibberellin metabolites are overwhelmingly difficult for their similar structures. Using in vitro biochemical approach, our work demonstrates for the first time that the synthetic GAI N-terminal DELLA domain peptides have similar bioactive function as the expressed protein to interact with AtGID1a receptor. Furthermore, our results reveal that DELLA motif is vitally important region and DELLA segment is essentially required region to recognize AtGID1a receptor. Finally, based on bioactive GA-dependent of the interaction between AtGID1a and DELLA protein, we generated a new method that could identify and detect bioactive GAs accurately and rapidly with surface plasmon resonance assays. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Development of a radioreceptor assay for human chorion gonadotropin: Application in normal and pathological pregnancies

    International Nuclear Information System (INIS)

    Koch, R.

    1978-01-01

    Rats testes were homogenised, and the binding capacity of several dilutions of these were tested with iodine 125 -labelled human choriongonadotropin. Investigations about binding over a period of 36 hrs. with 3 different temperatures, inhibition tests and cross reaction analyses for determining the specificity were carried out. 2 assay systems could be developed. The highly sensitive assay was applied at early pregnancy, at suspected disturbed or ectopic gravidity and allowed to measure the hCG-serum concentration above the physiological basal secretion of hLH. The less sensitive assay was used for measuring hCG in later stages of pregnancy, chorionepitheliomas and other hCG producing tumours. With the highly sensitive and specific assay, hCG was determinable 8 to 10 days post conceptionem. (orig.) [de

  7. Development of a replication-competent lentivirus assay for dendritic cell-targeting lentiviral vectors

    Directory of Open Access Journals (Sweden)

    Daniel C Farley

    Full Text Available It is a current regulatory requirement to demonstrate absence of detectable replication-competent lentivirus (RCL in lentiviral vector products prior to use in clinical trials. Immune Design previously described an HIV-1-based integration-deficient lentiviral vector for use in cancer immunotherapy (VP02. VP02 is enveloped with E1001, a modified Sindbis virus glycoprotein which targets dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN expressed on dendritic cells in vivo. Vector enveloped with E1001 does not transduce T-cell lines used in standard HIV-1-based RCL assays, making current RCL testing formats unsuitable for testing VP02. We therefore developed a novel assay to test for RCL in clinical lots of VP02. This assay, which utilizes a murine leukemia positive control virus and a 293F cell line expressing the E1001 receptor DC-SIGN, meets a series of evaluation criteria defined in collaboration with US regulatory authorities and demonstrates the ability of the assay format to amplify and detect a hypothetical RCL derived from VP02 vector components. This assay was qualified and used to test six independent GMP production lots of VP02, in which no RCL was detected. We propose that the evaluation criteria used to rationally design this novel method should be considered when developing an RCL assay for any lentiviral vector.

  8. Developing technologies for lunar-based astronomy

    Science.gov (United States)

    Johnson, Stewart W.; Burns, Jack O.; Chua, Koon Meng; Wetzel, John P.

    1992-01-01

    Prospects for lunar-based astronomy and the development of the required technologies are briefly reviewed. A systematic approach to lunar-based astronomy includes a progression in capability from small automated telescopes to the 16-meter reflector on the moon. A next step beyond the 16-meter reflector will be a Lunar Optical/Ultraviolet/Infrared Synthesis Array. Intermediate steps are represented by the Lunar Transit Telescope and the Lunar Cluster Telescope Experiment. Priorities for the required technology development are identified.

  9. Global Nuclear Energy Partnership Technology Development Plan

    Energy Technology Data Exchange (ETDEWEB)

    David J. Hill

    2007-07-01

    This plan describes the GNEP Technology Demonstration Program (GNEP-TDP). It has been prepared to guide the development of integrated plans and budgets for realizing the domestic portion of the GNEP vision as well as providing the basis for developing international cooperation. Beginning with the GNEP overall goals, it describes the basic technical objectives for each element of the program, summarizes the technology status and identifies the areas of greatest technical risk. On this basis a proposed technology demonstration program is described that can deliver the required information for a Secretarial decision in the summer of 2008 and support construction of facilities.

  10. Developing Technological Capabilities in Agro-Industry

    DEFF Research Database (Denmark)

    Whitfield, Lindsay

    2012-01-01

    This article examines the emergence and trajectory of a new agro-industry in Ghana, the pineapple export industry, using the technological capabilities approach. It explains the limited expansion of the industry and its declining competitiveness in the face of new competition by looking at how...... Ghanaian exporters developed technological capabilities initially and the incentives and disincentives to building on those capabilities. The article argues that at the heart of the industry's crisis was an inability to further develop technological capabilities. The crisis had systemic features that have...

  11. Capitalizing on App Development Tools and Technologies

    Science.gov (United States)

    Luterbach, Kenneth J.; Hubbell, Kenneth R.

    2015-01-01

    Instructional developers and others creating apps must choose from a wide variety of app development tools and technologies. Some app development tools have incorporated visual programming features, which enable some drag and drop coding and contextual programming. While those features help novices begin programming with greater ease, questions…

  12. Banking, Technology Workers and Their Career Development.

    Science.gov (United States)

    Armstrong, Lesley; West, Jim

    2001-01-01

    An Australian bank developed a four-stage career development strategy for information technology workers: (1) career coaching sessions with executives; (2) career coaching seminars for line managers and team leaders; (3) staff career planning workshops; and (4) online career development support. The program resulted in increased satisfaction,…

  13. Mixed Waste Integrated Program emerging technology development

    International Nuclear Information System (INIS)

    Berry, J.B.; Hart, P.W.

    1994-01-01

    The US Department of Energy (DOE) is responsible for the management and treatment of its mixed low-level wastes (MLLW). MLLW are regulated under both the Resource Conservation and Recovery Act and various DOE orders. Over the next 5 years, DOE will manage over 1.2 m 3 of MLLW and mixed transuranic (MTRU) wastes. In order to successfully manage and treat these mixed wastes, DOE must adapt and develop characterization, treatment, and disposal technologies which will meet performance criteria, regulatory approvals, and public acceptance. Although technology to treat MLLW is not currently available without modification, DOE is committed to developing such treatment technologies and demonstrating them at the field scale by FY 1997. The Office of Research and Development's Mixed Waste Integrated Program (MWIP) within the DOE Office of Environmental Management (EM), OfFice of Technology Development, is responsible for the development and demonstration of such technologies for MLLW and MTRU wastes. MWIP advocates and sponsors expedited technology development and demonstrations for the treatment of MLLW

  14. Coherent Architecture Development as a Basis for Technology Development

    DEFF Research Database (Denmark)

    Ravn, Poul Martin

    coherent architectures in a technology context as a basis for identification of critical development areas, this research has been focused around the following three areas: 1. Product architecture instances for prototypes testing novel technology. 2. Product architecture definition for a sub-system based......The subject of this PhD thesis is architecture-centered design. It elaborates especially on two specific areas: the coherence in architectures in a technology development context and the identification of critical development areas via property-based reasoning, based on an understanding of cette...... coherence. Despite the acceptance and results presented in multiple studies from the application of architectures, the research on architecture work in a technology development context is limited. Technologies are often developed and represented in the form of product sub-systems that are made available...

  15. Development of maraviroc, a CCR5 antagonist for treatment of HIV, using a novel tropism assay.

    Science.gov (United States)

    van der Ryst, Elna; Heera, Jayvant; Demarest, James; Knirsch, Charles

    2015-06-01

    Assays to identify infectious organisms are critical for diagnosis and enabling the development of therapeutic agents. The demonstration that individuals with a 32-bp deletion within the CCR5 locus were resistant to human immunodeficiency virus (HIV) infection, while those heterozygous for the mutation progressed more slowly, led to the discovery of maraviroc (MVC), a CCR5 antagonist. As MVC is only active against CCR5-tropic strains of HIV, it was critical to develop a diagnostic assay to identify appropriate patients. Trofile™, a novel phenotypic tropism assay, was used to identify patients with CCR5-tropic virus for the MVC development program. Results of these clinical studies demonstrated that the assay correctly identified patients likely to respond to MVC. Over time, the performance characteristics of the phenotypic assay were enhanced, necessitating retesting of study samples. Genotypic tropism tests that have the potential to allow for local use and more rapid turnaround times are also being developed. © 2015 New York Academy of Sciences.

  16. SMD Technology Development Story for NASA Annual Technology report

    Science.gov (United States)

    Seablom, Michael S.

    2017-01-01

    The role of the Science Mission Directorate (SMD) is to enable NASA to achieve its science goals in the context of the Nation's science agenda. SMD's strategic decisions regarding future missions and scientific pursuits are guided by Agency goals, input from the science community-including the recommendations set forth in the National Research Council (NRC) decadal surveys-and a commitment to preserve a balanced program across the major science disciplines. Toward this end, each of the four SMD science divisions-Heliophysics, Earth Science, Planetary Science, and Astrophysics-develops fundamental science questions upon which to base future research and mission programs. Often the breakthrough science required to answer these questions requires significant technological innovation-e.g., instruments or platforms with capabilities beyond the current state of the art. SMD's targeted technology investments fill technology gaps, enabling NASA to build the challenging and complex missions that accomplish groundbreaking science.

  17. Development of an FgMito assay: A highly sensitive mitochondrial based qPCR assay for quantification of Fusarium graminearum sensu stricto.

    Science.gov (United States)

    Kulik, Tomasz; Ostrowska, Anna; Buśko, Maciej; Pasquali, Matias; Beyer, Marco; Stenglein, Sebastian; Załuski, Dariusz; Sawicki, Jakub; Treder, Kinga; Perkowski, Juliusz

    2015-10-01

    An ascomycete fungus, Fusarium graminearum sensu stricto (s.s.), is the major cause of Fusarium head blight (FHB), a devastating disease of cereals worldwide. The fungus contaminates crops with mycotoxins, which pose a serious threat to food and feed safety. In this study, we developed a highly sensitive mitochondrial based qPCR assay (FgMito qPCR) for quantification of F. graminearum s.s. To ensure high sensitivity of the assay, primers and a Minor-groove binding (MGB) probe were designed based on multi-copy mitochondrial DNA. The FgMito assay was successfully validated against a range of geographically diverse F. graminearum s.s. strains to ensure uniformity of the assay at an intraspecific level, as well as with other fungal species to ensure specificity. The assay was further evaluated in terms of efficiency and sensitivity against a test panel of different F. graminearum s.s. strains with various levels of pure fungal DNA and in the presence of wheat background DNA. The results showed a high efficiency of the assay developed, ranging from 93% to 101% with r(2)-values of >0.99. We further showed that three low concentrations of fungal template 2 pg, 0.6 pg and 0.2 pg could be reliably quantified in the presence of wheat background DNA. The FgMito assay was used to quantify F. graminearum s.s. DNA on 65 field samples from a range of hosts with defined levels of trichothecenes. We revealed a significant positive correlation between fungal DNA quantity and the sum of trichothecenes. Lastly, we showed a higher sensitivity of the FgMito assay than the nuclear based qPCR assay for F. graminearum s.s. by comparing Ct-values from both assays. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines.

    Science.gov (United States)

    Košir, Alexandra Bogožalec; Spilsberg, Bjørn; Holst-Jensen, Arne; Žel, Jana; Dobnik, David

    2017-08-17

    Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the world market, qPCR analysis becomes laborious and expensive. Innovative cost-effective approaches are therefore urgently needed. Here, we report the development and inter-laboratory assessment of multiplex assays to quantify GMO soybean using droplet digital PCR (ddPCR). The assays were developed to facilitate testing of foods and feed for compliance with current GMO regulations in the European Union (EU). Within the EU, the threshold for labelling is 0.9% for authorised GMOs per ingredient. Furthermore, the EU has set a technical zero tolerance limit of 0.1% for certain unauthorised GMOs. The novel multiplex ddPCR assays developed target 11 GMO soybean lines that are currently authorised, and four that are tolerated, pending authorisation in the EU. Potential significant improvements in cost efficiency are demonstrated. Performance was assessed for the critical parameters, including limits of detection and quantification, and trueness, repeatability, and robustness. Inter-laboratory performance was also determined on a number of proficiency programme and real-life samples.

  19. Technology certification and technology acceptance: Promoting interstate cooperation and market development for innovative technologies

    International Nuclear Information System (INIS)

    Brockbank, B.R.

    1995-03-01

    In the past two years, public and private efforts to promote development and deployment of innovative environmental technologies have shifted from the analysis of barriers to the implementation of a variety of initiatives aimed at surmounting those barriers. Particular attention has been directed at (1) streamlining fragmented technology acceptance processes within and among the states, and (2) alleviating disincentives, created by inadequate or unverified technology cost and performance data, for users and regulators to choose innovative technologies. Market fragmentation currently imposes significant cost burdens on technology developers and inhibits the investment of private capital in environmental technology companies. Among the responses to these problems are state and federal technology certification/validation programs, efforts to standardize cost/performance data reporting, and initiatives aimed at promoting interstate cooperation in technology testing and evaluation. This paper reviews the current status of these initiatives, identifies critical challenges to their success, and recommends strategies for addressing those challenges

  20. Development of ultrasensitive spectroscopic analysis technology

    International Nuclear Information System (INIS)

    Cha, Hyung Ki; Song, K. S.; Kim, D. H.; Yang, K. H.; Jung, E. C.; Jeong, D. Y.; Yi, Y. J.; Lee, S. M.; Hong, K. H.; Han, J. M.; Yoo, B. D.; Rho, S. P.; Yi, J. H.; Park, H. M.; Cha, B. H.; Nam, S. M.; Lee, J. M.

    1997-09-01

    For the development of the laser initiated high resolution, ultra sensitive analysis technology following field of researches have been performed. 1) Laser resonance ionization technology, 2) Laser-induced rare isotope detection technology, 3) Laser-induced plasma analysis technology, 4) Microparticle analysis technology by using ion trap, 5) Laser induced remote sensing technique. As a result a monitoring system for photoionized product is developed and the test of system is performed with Sm sample. The rare isotope detection system is designed and a few key elements of the system are developed. In addition a laser-induced plasma analysis system is developed and samples such as Zircaloy, Zinc-base alloy, rock samples are reasonably analyzed. The detection sensitivity is identified as good as a few ppm order. An ion trap is developed and microparticles such as SiC are trapped inside the trap by ac and dc fields. The fluorescence signals from the organic dyes as well as rare earth element which are absorbed on the microparticles are detected. Several calibration curves are also obtained. In the field of laser remote sensing a mobile Lidar system is designed and several key elements are developed. In addition the developed system is used for the detection of Ozone, NO 2 , SO 2 , etc. (author). 57 refs., 42 figs

  1. Development of ultrasensitive spectroscopic analysis technology

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Hyung Ki; Song, K. S.; Kim, D. H.; Yang, K. H.; Jung, E. C.; Jeong, D. Y.; Yi, Y. J.; Lee, S. M.; Hong, K. H.; Han, J. M.; Yoo, B. D.; Rho, S. P.; Yi, J. H.; Park, H. M.; Cha, B. H.; Nam, S. M.; Lee, J. M.

    1997-09-01

    For the development of the laser initiated high resolution, ultra sensitive analysis technology following field of researches have been performed. (1) Laser resonance ionization technology, (2) Laser-induced rare isotope detection technology, (3) Laser-induced plasma analysis technology, (4) Microparticle analysis technology by using ion trap, (5) Laser induced remote sensing technique. As a result a monitoring system for photoionized product is developed and the test of system is performed with Sm sample. The rare isotope detection system is designed and a few key elements of the system are developed. In addition a laser-induced plasma analysis system is developed and samples such as Zircaloy, Zinc-base alloy, rock samples are reasonably analyzed. The detection sensitivity is identified as good as a few ppm order. An ion trap is developed and microparticles such as SiC are trapped inside the trap by ac and dc fields. The fluorescence signals from the organic dyes as well as rare earth element which are absorbed on the microparticles are detected. Several calibration curves are also obtained. In the field of laser remote sensing a mobile Lidar system is designed and several key elements are developed. In addition the developed system is used for the detection of Ozone, NO{sub 2}, SO{sub 2}, etc. (author). 57 refs., 42 figs.

  2. Development of a nested-PCR assay for the detection of Cryptosporidium parvum in finished water.

    Science.gov (United States)

    Monis, P T; Saint, C P

    2001-05-01

    A nested-PCR assay, incorporating an internal positive control, was developed for Cryptosporidium monitoring in finished water. This assay was capable of reproducibly detecting 8 oocysts in spiked-filtered water samples collected from 5 South Australian water treatment plants. The RT-PCR assay of Kaucner and Stinear (Appl. Environ. Microbiol. 64(5) (1998) 1743) was also evaluated for the detection of Cryptosporidium parvum. Initially, under our experimental conditions, a detection level of 27 oocysts was achieved for spiked reagent water samples. This level was improved to 5 oocysts by modification of the method. Untreated South Australian source waters concentrated by calcium carbonate flocculation were found to be highly inhibitory to the RT-PCR assay. Concentration of similar samples using Envirochek filters appeared to eliminate PCR inhibition. While both methods possessed similar sensitivities the nested-PCR assay was more reproducible, more cost effective, simpler to perform and could detect both viable and non-viable intact Cryptosporidium parvum oocysts, which is an important consideration for plant operators. These factors make the nested-PCR assay the method of choice for screening large numbers of potable water samples, where a reliable low level of detection is essential.

  3. Development and optimization of a direct plaque assay for human and avian metapneumoviruses

    Science.gov (United States)

    Zhang, Yu; Wei, Yongwei; Li, Junan; Li, Jianrong

    2012-01-01

    The genus Metapneumovirus within the subfamily Pneumovirinae and family Paramyxoviridae includes only two viruses, human metapneumovirus (hMPV) and avian metapneumovirus (aMPV), which cause respiratory disease in humans and birds, respectively. These two viruses grow poorly in cell culture and other quantitation methods, such as indirect immuno-staining and immuno-fluorescent assays, are expensive, time consuming, and do not allow for plaque purification of the virus. In order to enhance research efforts for studying these two viruses, a direct plaque assay for both hMPV and aMPV has been developed. By optimizing the chemical components of the agarose overlay, it was found that both hMPV with a trypsin-independent F cleavage site and aMPV formed clear and countable plaques in a number of mammalian cell lines (such as Vero-E6 and LLC-MK2 cells) after 5 days of incubation. The plaque forming assay has similar sensitivity and reliability as the currently used immunological methods for viral quantitation. The plaque assay is also a more simple, rapid, and economical method compared to immunological assays, and in addition allows for plaque purification of the viruses. The direct plaque assay will be a valuable method for the quantitation and evaluation of the biological properties of some metapneumoviruses. PMID:22684013

  4. CROSSCUTTING TECHNOLOGY DEVELOPMENT AT THE CENTER FOR ADVANCED SEPARATION TECHNOLOGIES

    Energy Technology Data Exchange (ETDEWEB)

    Christopher E. Hull

    2005-01-20

    The U.S. is the largest producer of mining products in the world. In 2003, U.S. mining operations produced $57 billion worth of raw materials that contributed a total of $564 billion to the nation's wealth. Despite these contributions, the mining industry has not been well supported with research and development funds as compared to mining industries in other countries. To overcome this problem, the Center for Advanced Separation Technologies (CAST) was established to develop technologies that can be used by the U.S. mining industry to create new products, reduce production costs, and meet environmental regulations. Much of the research to be conducted with Cooperative Agreement funds will be longer-term, high-risk, basic research and will be carried out in five broad areas: (1) Solid-solid separation; (2) Solid-liquid separation; (3) Chemical/Biological Extraction; (4) Modeling and Control; and (5) Environmental Control.

  5. Research and development and management of technology

    International Nuclear Information System (INIS)

    Kim, Yeong Gil

    1989-04-01

    This book mentions current state of affairs on research and development and prospect : activity of business, field like information and materials, energy and resource, public welfare, general industry technology. It introduces policy on promotion of research and development such as propel of special research and development business, propel strategy for 10 priority tasks, reinforcement of basic research, promotion of information industry and propel for technical development of business.

  6. HUMID AIR TURBINE CYCLE TECHNOLOGY DEVELOPMENT PROGRAM

    Energy Technology Data Exchange (ETDEWEB)

    Richard Tuthill

    2002-07-18

    The Humid Air Turbine (HAT) Cycle Technology Development Program focused on obtaining HAT cycle combustor technology that will be the foundation of future products. The work carried out under the auspices of the HAT Program built on the extensive low emissions stationary gas turbine work performed in the past by Pratt & Whitney (P&W). This Program is an integral part of technology base development within the Advanced Turbine Systems Program at the Department of Energy (DOE) and its experiments stretched over 5 years. The goal of the project was to fill in technological data gaps in the development of the HAT cycle and identify a combustor configuration that would efficiently burn high moisture, high-pressure gaseous fuels with low emissions. The major emphasis will be on the development of kinetic data, computer modeling, and evaluations of combustor configurations. The Program commenced during the 4th Quarter of 1996 and closed in the 4th Quarter of 2001. It teamed the National Energy Technology Laboratory (NETL) with P&W, the United Technologies Research Center (UTRC), and a subcontractor on-site at UTRC, kraftWork Systems Inc. The execution of the program started with bench-top experiments that were conducted at UTRC for extending kinetic mechanisms to HAT cycle temperature, pressure, and moisture conditions. The fundamental data generated in the bench-top experiments was incorporated into the analytical tools available at P&W to design the fuel injectors and combustors. The NETL then used the hardware to conduct combustion rig experiments to evaluate the performance of the combustion systems at elevated pressure and temperature conditions representative of the HAT cycle. The results were integrated into systems analysis done by kraftWork to verify that sufficient understanding of the technology had been achieved and that large-scale technological application and demonstration could be undertaken as follow-on activity. An optional program extended the

  7. Advances in Robotic Servicing Technology Development

    Science.gov (United States)

    Gefke, Gardell G.; Janas, Alex; Pellegrino, Joseph; Sammons, Matthew; Reed, Benjamin

    2015-01-01

    NASA's Satellite Servicing Capabilities Office (SSCO) has matured robotic and automation technologies applicable to in-space robotic servicing and robotic exploration over the last six years. This paper presents the progress of technology development activities at the Goddard Space Flight Center Servicing Technology Center and on the ISS, with an emphasis on those occurring in the past year. Highlighted advancements are design reference mission analysis for servicing in low Earth orbit (LEO) and asteroid redirection; delivery of the engineering development unit of the NASA Servicing Arm; an update on International Space Station Robotic Refueling Mission; and status of a comprehensive ground-based space robot technology demonstration expanding in-space robotic servicing capabilities beginning fall 2015.

  8. Fission Surface Power Technology Development Status

    Science.gov (United States)

    Palac, Donald T.; Mason, Lee S.; Houts, Michael G.; Harlow, Scott

    2010-01-01

    Power is a critical consideration in planning exploration of the surfaces of the Moon, Mars, and beyond. Nuclear power is an important option, especially for locations in the solar system where sunlight is limited in availability or intensity. NASA is maintaining the option for fission surface power for the Moon and Mars by developing and demonstrating technology for an affordable fission surface power system. Because affordability drove the determination of the system concept that this technology will make possible, low development and recurring costs result, while required safety standards are maintained. However, an affordable approach to fission surface power also provides the benefits of simplicity, robustness, and conservatism in design. This paper will illuminate the multiplicity of benefits to an affordable approach to fission surface power, and will describe how the foundation for these benefits is being developed and demonstrated in the Exploration Technology Development Program s Fission Surface Power Project.

  9. Trends in Wind Energy Technology Development

    DEFF Research Database (Denmark)

    Rasmussen, Flemming; Madsen, Peter Hauge; Tande, John O.

    2011-01-01

    . The development of new and larger turbines to some extent stagnated, and costs even rose due to high demand and rising materials costs. We believe, however – and this is supported by recent trends – that the next decade will be a new period of technology development and further scale-up, leading to more cost-effective....... The huge potential of wind, the rapid development of the technology and the impressive growth of the industry justify the perception that wind energy is changing its role to become the future backbone of a secure global energy supply. Between the mid-1980s, when the wind industry took off, and 2005 wind...... turbine technology has seen rapid development, leading to impressive increases in the size of turbines, with corresponding cost reductions. From 2005 to 2009 the industry’s focus seems to have been on increasing manufacturing capacity, meeting market demand and making wind turbines more reliable...

  10. Technological opportunities and paths of development

    DEFF Research Database (Denmark)

    Plichta, Kirsten

    1993-01-01

    in the industry. 6) It is argued that such paths of incremental improvement at the industry level may be an outcome of a) the dynamics that produce the technological opportunities; b) the institutions that govern decisions and expectations and c) the criteria by which the chooses between different firms...... technological development efforts. 7) The dynamic that produces opportunities for technological development will in part depend on characteristics of the product (e.g., its complexity), the interdependence between the product and production techniques, the diffusion of the product and in part...... the outcome of different firms development effort may also help shape a path at the industry level. This may be because the criteria by which the market selects between the different product may to some extent be anticipated by the developing firms or because the criteria by which the market select betwee...

  11. Developing technology pushed breakthroughs: an empirical study

    Directory of Open Access Journals (Sweden)

    Jari Sarja

    2017-12-01

    Full Text Available Developing a technology push product that brings real novelty to the market is difficult, risky and costly. This case study analyzes success factors defined by the literature. True industrial cases, representing Finnish ICT firms in their early phase after a successful market entry, were researched for the success factor analysis. The whole set of the previously introduced success factors were variably supported, and three new factors arose. Because the technology pushed development processes are risky with high failure rates, the validated success factors are valuable knowledge for the developments intensive firm’s management.

  12. Development and assembly of equipment for non destructive assay system control using nuclear radiation

    International Nuclear Information System (INIS)

    Melo, Jose Altino Tupinamba

    2006-01-01

    Nondestructive Assay (NDA) is applied to machines and components quality tests. These elements would not have a good performance if they were conceived without concern about the mechanical project quality, used materials, manufacture processes and inspection and maintenance methodology. There are constant developments in high level of technology with the objective of guaranteeing the components quality and the good functioning of these machines, in the mechanics, naval, aeronautical, petrochemical and steel industry, energy and nuclear generation as well. The globalization in the industry lines is a fact, leading to an increase in the multinational projects and products. The following questions arise: how to assure the high quality of components and processes? How to optimize the test methods to assure that the materials do not have defects affecting the performance of the components? The answers to the questions above are found in the application of NDA. The complex materials analysis (inhomogeneous) using NDA requires a detailed study of the sensors response signal. In this work, a measure and control system of non destructive processes was developed, using a radioactive source with a defined energy in function of the material to be analyzed. This system involves: (a) Interface of input/output (I/O) (the Hardware) and (b) graphical Interface (Software). In the non destructive analysis, it is made the comparison of the signal proceeding from the sensor with a signal preset (Set Point) or analogical signal of reference (Base Line), which is adjusted in the I/O Interface. Analyzed the signal, the system will make the decision: (a) to reject or (b) to accept the analyzed material. The I/O Interface is implemented by electronic equipment with a MCS51. The purpose of this interface is to supply conditions to exchange information, using serial RS232, between the sensor and the microcomputer. The graphical Interface (software) is written in visual C++ language (author)

  13. Music, Technology and National Development: Rethinking Some ...

    African Journals Online (AJOL)

    There is equally the tacit acceptance of the importance of the role of technology for music in particular and development in general. This paper examines the background assumptions that set in motion the conference theme, to find out what music can genuinely contribute to national development, given the trajectories which ...

  14. Developing Industrial and Technological Manpower via Technical ...

    African Journals Online (AJOL)

    In line with the assertion, this study identified the mechanisms for developing industrial and technological manpower via TVET for national development. The study was guided with two research questions and one hypothesis. The descriptive survey research design was employed. The sample was made up of 135 TVET ...

  15. New Achievements in Technology Education and Development

    Science.gov (United States)

    Soomro, Safeeullah, Ed.

    2010-01-01

    Since many decades Education Science and Technology has an achieved tremendous recognition and has been applied to variety of disciplines, mainly Curriculum development, methodology to develop e-learning systems and education management. Many efforts have been taken to improve knowledge of students, researchers, educationists in the field of…

  16. Development of coal hydro gasification technology

    International Nuclear Information System (INIS)

    Itoh, Kazuo; Nomura, Kazuo; Asaoka, Yoshikiyo; Kato, Shojiro; Seo, Tomoyuki

    1997-01-01

    Taking a potential future decrease in natural gas supply into consideration, we are looking for a way to secure a stable supply of high quality substitute natural gas made from coal (which occurs abundantly throughout the world) in large volumes at low cost. We are working towards our goal of commercializing coal hydro gasification technology in the 2010's and have started developing elemental technology from FY, 1996 as a part of the governmental new energy program. (au)

  17. Development of a central nervous system axonal myelination assay for high throughput screening.

    Science.gov (United States)

    Lariosa-Willingham, Karen D; Rosler, Elen S; Tung, Jay S; Dugas, Jason C; Collins, Tassie L; Leonoudakis, Dmitri

    2016-04-22

    Regeneration of new myelin is impaired in persistent multiple sclerosis (MS) lesions, leaving neurons unable to function properly and subject to further degeneration. Current MS therapies attempt to ameliorate autoimmune-mediated demyelination, but none directly promote the regeneration of lost and damaged myelin of the central nervous system (CNS). Development of new drugs that stimulate remyelination has been hampered by the inability to evaluate axonal myelination in a rapid CNS culture system. We established a high throughput cell-based assay to identify compounds that promote myelination. Culture methods were developed for initiating myelination in vitro using primary embryonic rat cortical cells. We developed an immunofluorescent phenotypic image analysis method to quantify the morphological alignment of myelin characteristic of the initiation of myelination. Using γ-secretase inhibitors as promoters of myelination, the optimal growth, time course and compound treatment conditions were established in a 96 well plate format. We have characterized the cortical myelination assay by evaluating the cellular composition of the cultures and expression of markers of differentiation over the time course of the assay. We have validated the assay scalability and consistency by screening the NIH clinical collection library of 727 compounds and identified ten compounds that promote myelination. Half maximal effective concentration (EC50) values for these compounds were determined to rank them according to potency. We have designed the first high capacity in vitro assay that assesses myelination of live axons. This assay will be ideal for screening large compound libraries to identify new drugs that stimulate myelination. Identification of agents capable of promoting the myelination of axons will likely lead to the development of new therapeutics for MS patients.

  18. Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents.

    Science.gov (United States)

    Euler, Milena; Wang, Yongjie; Heidenreich, Doris; Patel, Pranav; Strohmeier, Oliver; Hakenberg, Sydney; Niedrig, Matthias; Hufert, Frank T; Weidmann, Manfred

    2013-04-01

    Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents. The panel included RPAs for Francisella tularensis, Yersinia pestis, Bacillus anthracis, variola virus, and reverse transcriptase RPA (RT-RPA) assays for Rift Valley fever virus, Ebola virus, Sudan virus, and Marburg virus. Their analytical sensitivities ranged from 16 to 21 molecules detected (probit analysis) for the majority of RPA and RT-RPA assays. A magnetic bead-based total nucleic acid extraction method was combined with the RPAs and tested using inactivated whole organisms spiked into plasma. The RPA showed comparable sensitivities to real-time RCR assays in these extracts. The run times of the assays at 42°C ranged from 6 to 10 min, and they showed no cross-detection of any of the target genomes of the panel nor of the human genome. The RPAs therefore seem suitable for the implementation of syndromic panels onto microfluidic platforms.

  19. AFCI Safeguards Enhancement Study: Technology Development Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Leon E.; Dougan, A.; Tobin, Stephen; Cipiti, B.; Ehinger, Michael H.; Bakel, A. J.; Bean, Robert; Grate, Jay W.; Santi, P.; Bryan, Steven; Kinlaw, M. T.; Schwantes, Jon M.; Burr, Tom; Lehn, Scott A.; Tolk, K.; Chichester, David; Menlove, H.; Vo, D.; Duckworth, Douglas C.; Merkle, P.; Wang, T. F.; Duran, F.; Nakae, L.; Warren, Glen A.; Friedrich, S.; Rabin, M.

    2008-12-31

    The Advanced Fuel Cycle Initiative (AFCI) Safeguards Campaign aims to develop safeguards technologies and processes that will significantly reduce the risk of proliferation in the U.S. nuclear fuel cycle of tomorrow. The Safeguards Enhancement Study was chartered with identifying promising research and development (R&D) directions over timescales both near-term and long-term, and under safeguards oversight both domestic and international. This technology development roadmap documents recognized gaps and needs in the safeguarding of nuclear fuel cycles, and outlines corresponding performance targets for each of those needs. Drawing on the collective expertise of technologists and user-representatives, a list of over 30 technologies that have the potential to meet those needs was developed, along with brief summaries of each candidate technology. Each summary describes the potential impact of that technology, key research questions to be addressed, and prospective development milestones that could lead to a definitive viability or performance assessment. Important programmatic linkages between U.S. agencies and offices are also described, reflecting the emergence of several safeguards R&D programs in the U.S. and the reinvigoration of nuclear fuel cycles across the globe.

  20. Development of technologies for utilizing geothermal energy

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    In verifying the effectiveness of the deep geothermal resource exploration technology, development is being carried out on a fracture-type reservoir exploration method. The seismic exploration method investigates detailed structures of underground fracture systems by using seismic waves generated on the ground surface. Verification experiments for fiscal 1994 were carried out by selecting the Kakkonda area in which small fracture networks form reservoir beds. Geothermal resources in deep sections (deeper than 2000 m with temperatures higher than 350{degree}C) are promising in terms of amount of the resources, but anticipated with difficulty in exploration and impediments in drilling. To avoid these risks, studies are being progressed on the availability of resources in deep sections, their utilization possibility, and technologies of effective exploration and drilling. This paper summarizes the results of deep resource investigations during fiscal 1994. It also describes such technological development as hot water utilizing power generation. Development is performed on a binary cycle power generation plant which pumps and utilizes hot water of 150 to 200{degree}C by using a downhole pump. The paper also reports development on element technologies for hot rock power generation systems. It also dwells on development of safe and effective drilling and production technologies for deep geothermal resources.

  1. The microculture-kinetic (MiCK) assay: the role of a drug-induced apoptosis assay in drug development and clinical care.

    Science.gov (United States)

    Bosserman, Linda; Prendergast, Franklyn; Herbst, Roy; Fleisher, Martin; Salom, Emery; Strickland, Steven; Raptis, Anastasios; Hallquist, Allan; Perree, Mathieu; Rajurkar, Swapnil; Karimi, Misagh; Rogers, Karl; Davidson, Dirk; Willis, Carl; Penalver, Manuel; Homesley, Howard; Burrell, Matthew; Garrett, Audrey; Rutledge, James; Chernick, Michael; Presant, Cary A

    2012-08-15

    A drug-induced apoptosis assay, termed the microculture-kinetic (MiCK) assay, has been developed. Blinded clinical trials have shown higher response rates and longer survival in groups of patients with acute myelocytic leukemia and epithelial ovarian cancer who have been treated with drugs that show high apoptosis in the MiCK assay. Unblinded clinical trials in multiple tumor types have shown that the assay will be used frequently by clinicians to determine treatment, and when used, results in higher response rates, longer times to relapse, and longer survivals. Model economic analyses suggest possible cost savings in clinical use based on increased generic drug use and single-agent substitution for combination therapies. Two initial studies with drugs in development are promising. The assay may help reduce costs and speed time to drug approval. Correlative studies with molecular biomarkers are planned. This assay may have a role both in personalized clinical therapy and in more efficient drug development. ©2012 AACR.

  2. Crosscutting Technology Development at the Center for Advanced Separation Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Christopher Hull

    2009-10-31

    The U.S. is the largest producer of mining products in the world. In 2003, U.S. mining operations produced $57 billion worth of raw materials that contributed a total of $564 billion to the nation's wealth. Despite these contributions, the mining industry has not been well supported with research and development funds as compared to mining industries in other countries. To overcome this problem, the Center for Advanced Separation Technologies (CAST) was established to develop technologies that can be used by the U.S. mining industry to create new products, reduce production costs, and meet environmental regulations. Originally set up by Virginia Tech and West Virginia University, this endeavor has been expanded into a seven-university consortium -- Virginia Tech, West Virginia University, University of Kentucky, University of Utah, Montana Tech, New Mexico Tech and University of Nevada, Reno - that is supported through U.S. DOE Cooperative Agreement No. DE-FC26-02NT41607: Crosscutting Technology Development at the Center for Advanced Separation Technologies. Much of the research to be conducted with Cooperative Agreement funds will be longer-term, high-risk, basic research and will be carried out in five broad areas: (1) Solid-solid separation; (2) Solid-liquid separation; (3) Chemical/biological extraction; (4) Modeling and control; and (5) Environmental control. Distribution of funds is handled via competitive solicitation of research proposals through Site Coordinators at the seven member universities. These were first reviewed and ranked by a group of technical reviewers (selected primarily from industry). Based on these reviews, and an assessment of overall program requirements, the CAST Technical Committee made an initial selection/ranking of proposals and forwarded these to the DOE/NETL Project Officer for final review and approval. The successful projects are listed by category, along with brief abstracts of their aims and objectives.

  3. Wind technology development: Large and small turbines

    Science.gov (United States)

    Thresher, R. W.; Hock, S. M.; Loose, R. R.; Goldman, P.

    1994-12-01

    Wind technology has developed rapidly over the last decade with the design and development of advanced systems with improved performance, higher reliability, and lower costs. During the past several years, substantial gains have been made in wind turbine designs, lowering costs to an average of $0.05/kWh while further technology development is expected to allow the cost to drop below $0.04/kWh by 2000. As a result, wind is expected to be one of the least expensive forms of new electric generation in the next century. This paper will present the technology developments for both utility-scale wind turbines and remote, small-village wind turbines that are currently available or in development. Technology innovations are being adapted for remote and stand-alone power applications with smaller wind turbines. Hybrid power systems using smaller 1 to 50 (kW) wind turbines are being developed for non-grid-connected electrical generation applications. These village power systems typically use wind energy, photovoltaics, battery storage, and conventional diesel generators to power remote communities. Smaller turbines are being explored for application as distributed generation sources on utility grids to supply power during periods of peak demand, avoiding costly upgrades in distribution equipment. New turbine designs now account for turbulence-induced loads, unsteady aerodynamic stall effects, and complex fatigue loads, making use of new technology developments such as advanced airfoils. The new airfoils increase the energy capture, improve the operating efficiency, and reduce the sensitivity of the airfoils to operation roughness. Electronic controls are allowing variable rotor speed operation; while aerodynamic control devices, such as ailerons and flaps, are used to modulate power or stop the rotor in high-speed conditions. These technology trends and future turbine configurations are being sponsored and explored by the U.S. Department of Energy's Wind Energy Program.

  4. Development of an assay for a biomarker of pregnancy and early fetal loss

    International Nuclear Information System (INIS)

    Canfield, R.E.; O'Connor, J.F.; Birken, S.; Krichevsky, A.; Wilcox, A.J.

    1987-01-01

    Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; α and β, with molecular weights of 14 kD and 23 kD, respectively. Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH. Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. Antibody cooperative effects, in conjunction with kinetic advantages derived from the concentration factors by use of the sandwich assay technique (immunoradiometric assay, IRMA), have enabled development of extremely sensitive and specific measurement protocols for urinary hCG. The assay described herein permits the detection of pregnancy on an average 25.4 days after the first day of the preceding menses, as opposed to 29.5 days for conventional radioimmunoassay techniques. In addition, the greater sensitivity and specificity of this assay method has permitted the detection of episodes of fetal loss not detected by radioimmunoassay of urine specimens. A large scale epidemiological study is in progress using this assay technique as a way to identify pregnancies that are lost before becoming clinically apparent

  5. Development of industrial ion implantation technology

    International Nuclear Information System (INIS)

    Choi, Byung Ho; Hwang, Churl Kew; Kim, Wan; Jin, Jung Tai; Jung, Ki Sok; Yoon, Su Ho; Shin, Won Churl; Kim, Jong Gook; Han, Jeon Geon; Chung, Ki Hyung

    1994-01-01

    On a cooperation between KAERI, Kurchatov Institute (Russia), and Mirae Co., development of a metal ion implanter and ion implantation technology is performed on a basic idea of popularization and refinement of ion implantation technology applied to the industrial components. The developed implanter is a two beam type: the mass separation line produces several mA of metal ion beams and the non-separation line produces several tens of mA gas ion beams, thus making the synergistic effect possible by the irradiation of beams from both lines. The target is made of a rotating plate of 60cm in radius and can treat various types of industrial components or parts. About 60 kinds of specimens were treated for the development of implantation technology. Two or five times lengthening of longevities were achieved on the PCB drills, razor blades, cutters, and precision dies. (Author)

  6. Development of national safeguards inspection technology

    Energy Technology Data Exchange (ETDEWEB)

    Kwack, Eun Ho; You, Keon Jung; Kim, Jin Su [and others

    1995-12-01

    It is considered that the safeguards trend in the IAEA and international nonproliferation regime is being accelerated toward strengthened safeguards system. In order to effectively respond to the stream as a whole, government has to have a firm policy-intention on nuclear non-proliferation, and supporting stance as to the stream should be taken consistently. Also, technological development satisfying the non-proliferation requirement would be pursued for the establishment of transparency and the enhancement of international confidence. At present, Korea stands at take-off stage in terms of safeguards. Therefore, necessary measures such as arrangement of legal framework, staffing and training of inspection man-power, and purchase of inspection equipment should be taken at the same time for earlier settlement of national safeguards system. In this connection, international cooperation with the share of the inspection results and equipment between the IAEA and TCNC is demanded. In the long term, the development of inspection technology as well as the research of sophisticated technology will have to be pursued. Emphasis should be placed on the regional safeguards system as well. In addition, it is necessary to ensure internationally that nuclear technological development to be planned is development of safeguards technology. (author). 20 tabs., 24 figs., 45 refs.

  7. ISV technology development plan for buried waste

    International Nuclear Information System (INIS)

    Nickelson, D.F.; Callow, R.A.; Luey, J.K.

    1992-07-01

    This report identifies the main technical issues facing the in situ vitrification (ISV) application to buried waste, and presents a plan showing the top-level schedule and projected resources needed to develop and demonstrate the technology for meeting Environmental Restoration Department (ERD) needs. The plan also proposes a model strategy for the technology transfer from the Department of Energy's Office of Technology Development (DOE-OTD) to the Office of Environmental Restoration (DOE-ER) as the technology proceeds from issues resolution (development) to demonstration and remedial readiness. Implementation of the plan would require $34,91 1K in total funding to be spread in the years FY-93 through FY-98. Of this amount, $10,183K is planned to be funded by DOE-OTD through the ISV Integrated Program. The remaining amount, $24,728K, is recommended to be split between the Department of Energy (DOE) Office of Technology Development ($6,670K) and DOE Office of Environmental Restoration ($18,058K)

  8. Development of an Enzyme Linked Immunosorbent Assay to Detect Chicken Parvovirus Specific Antibodies

    Science.gov (United States)

    Here we report the development and application of an enzyme linked immunosorbent assay to detect parvovirus-specific antibodies in chicken sera. We used an approach previously described for other parvoviruses to clone and express viral structural proteins in insect cells from recombinant baculovirus...

  9. Development of a dipstick assay for detection of Leishmania-specific canine antibodies

    NARCIS (Netherlands)

    Schallig, Henk D. F. H.; Cardoso, Luís; Hommers, Marieke; Kroon, Nel; Belling, Guus; Rodrigues, Manuela; Semião-Santos, Saul J.; Vetter, Hans

    2004-01-01

    A dipstick assay, based on Leishmania infantum antigen, for the rapid detection of Leishmania-specific antibodies in canine serum samples was developed and evaluated. After determination of optimal dipstick test conditions, test performance was compared with two existing serological tests, i.e., the

  10. Development and evaluation of Taqman assays for the differentiation of Dickeya (sub)species

    NARCIS (Netherlands)

    Wolf, van der J.M.; Haas, de B.H.; Hoof, van R.A.; Haan, de E.G.; Bovenkamp, van den G.W.

    2014-01-01

    TaqMan assays were developed for the detection of seven Dickeya species, namely D. dianthicola, D. dadantii, D. paradisiaca, D. chrysanthemi, D. zeae, D. dieffenbachiae and D. solani. Sequences of the gene coding for dnaX were used for the design of primers and probes. In studies with axenic

  11. Analysis of lipoproteins by capillary zone electrophoresis in microfluidic devices: Assay development and surface roughness measurements

    NARCIS (Netherlands)

    Weiller, Bruce H.; Ceriotti, Laura; Shibata, Takayuki; Rein, Dietrich; Roberts, Matthew A.; Lichtenberg, Jan; German, J. Bruce; De Rooij, Nico F.; Verpoorte, Elisabeth

    2002-01-01

    The development of a new assay for lipoproteins by capillary electrophoresis in fused-silica capillaries and in glass microdevices is described in this paper. The separation of low-density (LDL) and high-density (HDL) lipoproteins by capillary zone electrophoresis is demonstrated in fused-silica

  12. Development of kit for immunoradiometric assay of prostate specific antigen: Preliminary results

    International Nuclear Information System (INIS)

    Benzaid, A.; Boudjemai, S.; Benhidour, A.; Saada, B.

    1999-01-01

    This report presents the preliminary results obtained in the development of immunoradiometric assay of Prostate specific Antigen. The paper describes the immobilisation of anti-PSA on polystyrene beads and tubers and 125 I labelling of anti-T-PSA and f-PSA monoclonal antibodies. The obtained results were encouraging and work is in progress. (author)

  13. Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron

    NARCIS (Netherlands)

    Suaréz-Pantaleón, C.; Wichers, J.H.; Abad-Somovilla, A.; Amerongen, van A.

    2013-01-01

    Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator

  14. Development of advanced neutron beam technology

    Energy Technology Data Exchange (ETDEWEB)

    Seong, B. S.; Lee, J. S.; Sim, C. M. (and others)

    2007-06-15

    The purpose of this work is to timely support the national science and technology policy through development of the advanced application techniques for neutron spectrometers, built in the previous project, in order to improve the neutron spectrometer techniques up to the world-class level in both quantity and quality and to reinforce industrial competitiveness. The importance of the research and development (R and D) is as follows: 1. Technological aspects - Development of a high value-added technology through performing the advanced R and D in the broad research areas from basic to applied science and from hard to soft condensed matter using neutron scattering technique. - Achievement of an important role in development of the new technology for the following industries aerospace, defense industry, atomic energy, hydrogen fuel cell etc. by the non-destructive inspection and analysis using neutron radiography. - Development of a system supporting the academic-industry users for the HANARO facility 2. Economical and Industrial Aspects - Essential technology in the industrial application of neutron spectrometer, in the basic and applied research of the diverse materials sciences, and in NT, BT, and IT areas - Broad impact on the economics and the domestic and international collaborative research by using the neutron instruments in the mega-scale research facility, HANARO, that is a unique source of neutron in Korea. 3. Social Aspects - Creating the scientific knowledge and contributing to the advanced industrial society through the neutron beam application - Improving quality of life and building a national consensus on the application of nuclear power by developing the RT fusion technology using the HANARO facility. - Widening the national research area and strengthening the national R and D capability by performing advanced R and D using the HANARO facility.

  15. Development of advanced neutron beam technology

    International Nuclear Information System (INIS)

    Seong, B. S.; Lee, J. S.; Sim, C. M.

    2007-06-01

    The purpose of this work is to timely support the national science and technology policy through development of the advanced application techniques for neutron spectrometers, built in the previous project, in order to improve the neutron spectrometer techniques up to the world-class level in both quantity and quality and to reinforce industrial competitiveness. The importance of the research and development (R and D) is as follows: 1. Technological aspects - Development of a high value-added technology through performing the advanced R and D in the broad research areas from basic to applied science and from hard to soft condensed matter using neutron scattering technique. - Achievement of an important role in development of the new technology for the following industries aerospace, defense industry, atomic energy, hydrogen fuel cell etc. by the non-destructive inspection and analysis using neutron radiography. - Development of a system supporting the academic-industry users for the HANARO facility 2. Economical and Industrial Aspects - Essential technology in the industrial application of neutron spectrometer, in the basic and applied research of the diverse materials sciences, and in NT, BT, and IT areas - Broad impact on the economics and the domestic and international collaborative research by using the neutron instruments in the mega-scale research facility, HANARO, that is a unique source of neutron in Korea. 3. Social Aspects - Creating the scientific knowledge and contributing to the advanced industrial society through the neutron beam application - Improving quality of life and building a national consensus on the application of nuclear power by developing the RT fusion technology using the HANARO facility. - Widening the national research area and strengthening the national R and D capability by performing advanced R and D using the HANARO facility

  16. Adherence of Candida albicans to bladder mucosa: development and application of a tissue explant assay.

    Science.gov (United States)

    Lyman, C A; Navarro, E; Garrett, K F; Roberts, D D; Pizzo, P A; Walsh, T J

    1999-01-01

    In order to study the interactions between Candida species and uroepithelial tissue, a tissue explant assay was developed using bladder mucosa harvested from New Zealand white rabbits. Blastoconidia of Candida albicans, Candida tropicalis and Candida glabrata attached to the uroepithelial tissue in similar quantities. However, there was significantly more adherence to the uroepithelium by pre-germinated C. albicans compared with C. albicans blastoconidia. Furthermore, the amount of uroepithelial tissue injury was directly related to the length of exposure of the tissue to Candida. Thus, this tissue explant assay may provide a useful method for investigating properties related to fungal adherence to transitional uroepithelium and organism-mediated tissue injury.

  17. Using ISS to develop telescope technology

    Science.gov (United States)

    Saenz-Otero, Alvar; Miller, David W.

    2005-08-01

    Future space telescope missions concepts have introduced new technologies such as precision formation flight, optical metrology, and segmented mirrors. These new technologies require demonstration and validation prior to deployment in final missions such as the James Webb Space Telescope, Terrestrial Planet Finder, and Darwin. Ground based demonstrations do not provide the precision necessary to obtain a high level of confidence in the technology; precursor free flyer space missions suffer from the same problems as the final missions. Therefore, this paper proposes the use of the International Space Station as an intermediate research environment where these technologies can be developed, demonstrated, and validated. The ISS provides special resources, such as human presence, communications, power, and a benign atmosphere which directly reduce the major challenges of space technology maturation: risk, complexity, cost, remote operations, and visibility. Successful design of experiments for use aboard the space station, by enabling iterative research and supporting multiple scientists, can further reduce the effects of these challenges of space technology maturation. This paper presents results of five previous MIT Space Systems Laboratory experiments aboard the Space Shuttle, MIR, and the ISS to illustrate successful technology maturation aboard these facilities.

  18. Highly sensitive detection of human IgG using a novel bio-barcode assay combined with DNA chip technology

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Zhenbao [Central South University, School of Pharmaceutical Sciences (China); Zhou, Bo, E-mail: zhoubo1771@163.com [The Affiliated Zhongda Hospital of Southeast University, Department of Gerontology (China); Wang, Haiqing; Lu, Feng; Liu, Tianjun; Song, Cunxian; Leng, Xigang, E-mail: lengxigyky@163.com [Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College (China)

    2013-09-15

    A simple and ultrasensitive detection of human IgG based on signal amplification using a novel bio-barcode assay and DNA chip technology was developed. The sensing platform was a sandwich system made up of antibody-modified magnetic microparticles (Ab-MMPs)/human IgG/Cy3-labeled single-stranded DNA and antibody-modified gold nanoparticles (Cy3-ssDNA-Ab-AuNPs). The MMPs (2.5 {mu}m in diameter) modified with mouse anti-human IgG monoclonal-antibodies could capture human IgG and further be separated and enriched via a magnetic field. The AuNPs (13 nm in diameter) conjugated with goat anti-human IgG polyclonal-antibodies and Cy3-ssDNA could further combine with the human IgG/Ab-MMP complex. The Cy3-ssDNA on AuNPs was then released by TCEP to hybridize with the DNA chip, thus generating a detectable signal by the fluorescence intensity of Cy3. In order to improve detection sensitivity, a three-level cascaded signal amplification was developed: (1) The MMP enrichment as the first-level; (2) Large quantities of Cy3-ssDNA on AuNPs as the second-level; (3) The Cy3-ssDNA conjugate with DNA chip as the third-level. The highly sensitive technique showed an increased response of the fluorescence intensity to the increased concentration of human IgG through a detection range from 1 pg mL{sup -1} to 10 ng mL{sup -1}. This sensing technique could not only improve the detection sensitivity for the low concentration of human IgG but also present a robust and efficient signal amplification model. The detection method has good stability, specificity, and reproducibility and could be applied in the detection of human IgG in the real samples.

  19. Making technological innovation work for sustainable development.

    Science.gov (United States)

    Anadon, Laura Diaz; Chan, Gabriel; Harley, Alicia G; Matus, Kira; Moon, Suerie; Murthy, Sharmila L; Clark, William C

    2016-08-30

    This paper presents insights and action proposals to better harness technological innovation for sustainable development. We begin with three key insights from scholarship and practice. First, technological innovation processes do not follow a set sequence but rather emerge from complex adaptive systems involving many actors and institutions operating simultaneously from local to global scales. Barriers arise at all stages of innovation, from the invention of a technology through its selection, production, adaptation, adoption, and retirement. Second, learning from past efforts to mobilize innovation for sustainable development can be greatly improved through structured cross-sectoral comparisons that recognize the socio-technical nature of innovation systems. Third, current institutions (rules, norms, and incentives) shaping technological innovation are often not aligned toward the goals of sustainable development because impoverished, marginalized, and unborn populations too often lack the economic and political power to shape innovation systems to meet their needs. However, these institutions can be reformed, and many actors have the power to do so through research, advocacy, training, convening, policymaking, and financing. We conclude with three practice-oriented recommendations to further realize the potential of innovation for sustainable development: (i) channels for regularized learning across domains of practice should be established; (ii) measures that systematically take into account the interests of underserved populations throughout the innovation process should be developed; and (iii) institutions should be reformed to reorient innovation systems toward sustainable development and ensure that all innovation stages and scales are considered at the outset.

  20. Making technological innovation work for sustainable development

    Science.gov (United States)

    Anadon, Laura Diaz; Harley, Alicia G.; Matus, Kira; Moon, Suerie; Murthy, Sharmila L.

    2016-01-01

    This paper presents insights and action proposals to better harness technological innovation for sustainable development. We begin with three key insights from scholarship and practice. First, technological innovation processes do not follow a set sequence but rather emerge from complex adaptive systems involving many actors and institutions operating simultaneously from local to global scales. Barriers arise at all stages of innovation, from the invention of a technology through its selection, production, adaptation, adoption, and retirement. Second, learning from past efforts to mobilize innovation for sustainable development can be greatly improved through structured cross-sectoral comparisons that recognize the socio-technical nature of innovation systems. Third, current institutions (rules, norms, and incentives) shaping technological innovation are often not aligned toward the goals of sustainable development because impoverished, marginalized, and unborn populations too often lack the economic and political power to shape innovation systems to meet their needs. However, these institutions can be reformed, and many actors have the power to do so through research, advocacy, training, convening, policymaking, and financing. We conclude with three practice-oriented recommendations to further realize the potential of innovation for sustainable development: (i) channels for regularized learning across domains of practice should be established; (ii) measures that systematically take into account the interests of underserved populations throughout the innovation process should be developed; and (iii) institutions should be reformed to reorient innovation systems toward sustainable development and ensure that all innovation stages and scales are considered at the outset. PMID:27519800

  1. Development of an enzyme-linked immunosorbent assay method to detect mustard protein in mustard seed oil

    NARCIS (Netherlands)

    Koppelman, S.J.; Vlooswijk, R.; Bottger, G.; Duijn, G. van; Schaft, P. van der; Dekker, J.; Bemgen, H. van

    2007-01-01

    An enzyme-linked immunosorbent assay for the detection of mustard protein was developed. The assay is based on a polyclonal antiserum directed against a mixture of mustard proteins raised in rabbits. The assay has a detection limit of 1.5 ppm (milligrams per kilogram) and is suitable for the

  2. Development of information technology: Ethical dimensions

    Directory of Open Access Journals (Sweden)

    Pećanac Slobodan

    2016-01-01

    Full Text Available Despite disagreements about whether the technology development in general (including information technology is something that is good or bad for the morale of the community and the individual, there is no dispute that this influence exists. Even when negatively affecting morale, these technologies do so only in the presence of other essential factors primarly and above all human intentions that go beyond the moral norms. So, no matter how much we are certain that information technologies themselves are not the main factor of immorality that does not mean that their use that leads to the 'chaos of immorality' should not be prevented. First of all, by strengthening of the moral community. In any case, mankind can not afford the luxury of hesitating when considering ethical questions posed to the fast development of technology and information transfer despite the pressures to shift all responsibility on lawyers and politicians. Certain legislation within the communities is inevitable and no doubt necessary, but solving ethical dilemmas in the field of new media should be left to the moral community and the reasonable judgment of all its members and not the exclusive regulatory powers of government. Defence of the communication ethics through new media is conditioned by the previously achieved results of efforts to defend the basic principles of sociability. Therefore, conscious efforts for establishing a much stronger social moral legally must lead to a reduction of moral chaos in all areas of communication and means of communication and new information technologies.

  3. Development of technologically important crystals and devices

    International Nuclear Information System (INIS)

    Singh, S.G.; Tyagi, M.; Desai, D.G.; Singh, A.K.; Tiwari, Babita; Sen, Shashwati; Chauhan, A.K.; Gadkari, S.C.

    2011-01-01

    Device-grade single crystals of technologically important materials for use as scintillators in nuclear radiation detection, lasers and optical devices are grown in Crystal Technology Section of the Technical Physics Division. The expertise has been developed to establish crystal growth processes and design and fabricate crystal growth equipment. In addition, single crystals of new materials to be used in neutron and gamma detection are also being studied and developed currently. The processing, characterization and testing of devices are carried out using in-house facilities as well as in collaboration with other Divisions. (author)

  4. Development of nuclear equipment qualification technology

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Heon O; Kim, Wu Hyun; Kim, Jin Wuk; Kim, Jeong Hyun; Lee, Jeong Kyu; Kim, Yong Han; Jeong, Hang Keun [Korea Institute of Machinery and Materials, Taejon (Korea)

    1999-03-01

    In order to enhance testing and evaluation technologies, which is one of the main works of the Chanwon branch of KIMM(Korea Institute of Machinery and Materials), in addition to the present work scope of the testing and evaluation in the industrial facilities such as petroleum and chemical, plants, the qualification technologies of the equipments important to safety used in the key industrial facilities such as nuclear power plants should be localized: Equipments for testing and evaluation is to be set up and the related technologies must be developed. In the first year of this study, of vibration aging qualification technologies of equipments important to safety used in nuclear power plants have been performed. (author). 27 refs., 81 figs., 17 tabs.

  5. Recent Technological Developments and Their Influence

    International Nuclear Information System (INIS)

    Neitzel, U.

    2000-01-01

    At present, medical X ray imaging is undergoing major changes triggered by important technological developments. Because of the widespread availability of powerful computers and recent advances in large-area electronics, digital imaging technologies are about to replace the last stronghold of conventional imaging, the radiographic film. An overview is given of the technological principles of the different digital image receptors and their relative merits. Another area where digital acquisition technology has already penetrated into clinical practice is fluoroscopy. Digital snapshots have virtually replaced the spot film. Grid controlled fluoroscopy allows the radiation pulses and thus the dose to be tailored according to the diagnostic question. Dose reductions of up to 80% are possible without loss of diagnostic information. The decoupling of detection and display in digital systems gives new freedom for optimising the imaging parameters but also requires new and appropriate optimisation strategies with respect to image quality and dose. (author)

  6. Development of multiplex-PCR assay for rapid detection of Candida spp.

    Directory of Open Access Journals (Sweden)

    Ni Made A. Tarini

    2010-05-01

    Full Text Available Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp.Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen® kit for Multiplex-PCR assay.Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of Candida spp. (Med J Indones 2010; 19:83-7Keywords: Candida spp., multiplex-PCR

  7. [Development of PCR assay for detection of Angiostrongylus cantonensis in Pomacea canaliculata].

    Science.gov (United States)

    Zhang, Yi; Zhou, Xiao-nong; Liu, He-xiang; Lv, Shan; Li, Li-sha; Lin, Jin-xiang; Li, You-song

    2006-10-01

    To establish a PCR assay for detecting the third-stage larvae of Angiostrongylus cantonensis in Pomacea canaliculata. Polymerase chain reaction primers were designed by the software Lasergene, based on the specific cDNA of the third-stage larvae of A.cantonensis in Genbank. The total RNA was prepared from the third-stage larvae of A.cantonensis and of the snails by TRIzol one-step protocol. Amplification by RT-PCR was carried out following the kit protocol. RT-PCR assay revealed a clear differentiation between infected and negative snails. When a mixture of the total RNA from the negative snails and the third-stage larvae of A.cantonensis was tested by the PCR assay, the detectable level was 128 pg RNA, a concentration close to one third-stage larva of A.cantonensis, minimum concentration that could be found by naked eyes. The minimum detected total RNA concentration of the third-stage larvae of A.cantonensis was 105 pg by PCR assay. A PCR assay has been developed for detecting A.cantonensis larva in Pomacea canaliculata.

  8. Development and validation of a rapid turbidimetric assay to determine the potency of norfloxacin in tablets

    Directory of Open Access Journals (Sweden)

    Lucas Chierentin

    2015-09-01

    Full Text Available Norfloxacin is one of the first commercially available (and most widely used fluoroquinolone antibiotics. This paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric assay method to quantify norfloxacin in tablets formulations in only 4 hours. The bioassay is based on the inhibitory effect of norfloxacin upon the strain ofStaphylococcus epidermidis ATCC 12228 IAL 2150 used as test microorganism. The assay was performed 3x3 parallel lines like, three tubes for each concentration of reference substance and three tubes for each sample concentration. The results were treated statistically by analysis of variance and were found to be linear (r2 = 0.9999 in the selected range of 25-100 μg mL-1; precise (intra-assay: relative standard deviation (RSD = 1.33%; inter-assay: RSD = 0.21%, accurate (100.74% and robust with RSD lower than 4.5%. The student's t-test showed no statistically significant difference between the proposed turbidimetric method and an HPLC method previously validated. However the turbidimetric assay can be used as a valuable alternative methodology for the routine quality control of this medicine, complementary to other physical-chemical methods.

  9. Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence.

    Directory of Open Access Journals (Sweden)

    Zachary J Farino

    Full Text Available Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.

  10. Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay.

    Science.gov (United States)

    Vasconcellos Cardoso, M; Blanchard, A; Ferris, S; Verlengia, R; Timenetsky, J; Florio Da Cunha, R A

    2000-03-15

    Ureaplasma diversum has been associated with different clinical manifestations including bovine vulvitis, endometritis, salpingitis, spontaneous abortion and infertility. Because the isolation of this ureaplasma from clinical samples is difficult, there is a need for improved detection methods. We developed a PCR assay based on amplification of a region of the gene encoding 16S rRNA. The specificity of the amplification was verified by sequence analysis. Female bovine vaginal swabs (n=168) were collected and the presence of U. diversum evaluated by both culture methods and by the PCR assay. Culture was positive for 60 samples (35.7%), and PCR-specific amplification was obtained for 89 samples (52.9%). These results indicated a high prevalence of U. diversum in the selected animals and the higher sensitivity of this PCR assay as compared to culture.

  11. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

    Directory of Open Access Journals (Sweden)

    Sam R. Nugen

    2012-01-01

    Full Text Available Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, with relevance in human and animal health in sub-Saharan Africa. The intensity of the chemiluminescent signal was evaluated by using a charge-coupled device as well as a microtiter plate reader. We demonstrated that our lateral flow chemiluminescent assay has a very low limit of detection and is easy to use. The limit of detection was determined to be 0.5 fmols of nucleic acid target.

  12. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

    Science.gov (United States)

    Wang, Yuhong; Fill, Catherine; Nugen, Sam R.

    2012-01-01

    Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, with relevance in human and animal health in sub-Saharan Africa. The intensity of the chemiluminescent signal was evaluated by using a charge-coupled device as well as a microtiter plate reader. We demonstrated that our lateral flow chemiluminescent assay has a very low limit of detection and is easy to use. The limit of detection was determined to be 0.5 fmols of nucleic acid target. PMID:25585630

  13. Development of a multiplex PCR assay for characterization of embryonic stem cells.

    Science.gov (United States)

    Pal, Rajarshi; Mamidi, Murali Krishna; Das, Anjan Kumar; Rao, Mahendra; Bhonde, Ramesh

    2013-01-01

    Several molecular methods like real-time PCR (Q-PCR), expression sequence tag (EST) scan, microarray and microRNA analysis, and massively parallel signature sequencing (MPSS) have proved to be increasingly sensitive and efficient for monitoring human embryonic stem cell (hESC) differentiation. However, most of these high-throughput tests have a limited use due to high cost, extended turnaround time, and the involvement of highly specialized technical expertise. Hence, there is a need of rapid, cost-effective, robust, yet sensitive method for routine screening of hESCs. A critical requirement in hESC cultures is to maintain a uniform undifferentiated state and to determine their differentiation capacity by showing the expression of germ-layer-specific gene markers. To determine the modulation of gene expression in hESCs during propagation, expansion, and differentiation via embryoid body (EB) formation, we developed a simple, rapid, inexpensive, and definitive multimarker, semiquantitative multiplex RT-PCR (mxPCR) platform technology. Among the 15 gene primers tested, 4 were pluripotent markers comprising of set 1; and 3 lineage-specific markers from each ecto-, meso-, and endoderm layers were combined as sets 2, 3, and 4, respectively. In summary, this study was performed to characterize hESCs on a molecular level and to determine the quality and degree of variability among hESC and their early progenies (EB). This single-reaction mxPCR assay was flexible and, by selecting appropriate reporter genes, can be designed for characterization of different hESC lines during routine maintenance and directed differentiation.

  14. Development of fabrication technology for CANDU advanced fuel -Development of the advanced CANDU technology-

    International Nuclear Information System (INIS)

    Choi, Chang Beom; Kim, Hyeong Soo; Kim, Sang Won; Seok, Ho Cheon; Shim, Ki Seop; Byeon, Taek Sang; Jang, Ho Il; Kim, Sang Sik; Choi, Il Kwon; Cho, Dae Sik; Sheo, Seung Won; Lee, Soo Cheol; Kim, Yoon Hoi; Park, Choon Ho; Jeong, Seong Hoon; Kang, Myeong Soo; Park, Kwang Seok; Oh, Hee Kwan; Jang, Hong Seop; Kim, Yang Kon; Shin, Won Cheol; Lee, Do Yeon; Beon, Yeong Cheol; Lee, Sang Uh; Sho, Dal Yeong; Han, Eun Deok; Kim, Bong Soon; Park, Cheol Joo; Lee, Kyu Am; Yeon, Jin Yeong; Choi, Seok Mo; Shon, Jae Moon

    1994-07-01

    The present study is to develop the advanced CANDU fuel fabrication technologies by means of applying the R and D results and experiences gained from localization of mass production technologies of CANDU fuels. The annual portion of this year study includes following: 1. manufacturing of demo-fuel bundles for out-of-pile testing 2. development of technologies for the fabrication and inspection of advanced fuels 3. design and munufacturing of fuel fabrication facilities 4. performance of fundamental studies related to the development of advanced fuel fabrication technology

  15. Foreign cooperative technology development and transfer

    International Nuclear Information System (INIS)

    Schassburger, R.J.; Robinson, R.A.

    1988-01-01

    It is the policy of the US Department of Energy (DOE) that, in pursuing the development of mined geologic repositories in the United States, the waste isolation program will continue to actively support international cooperation and exchange activities that are judged to be in the best interest of the program and in compliance with the Nuclear Waste Policy Act of 1982, Sec. 223. Because there are common technical issues and because technology development often requires large expenditures of funds and dedication of significant capital resources, it is advantageous to cooperate with foreign organizations carrying out similar activities. The DOE's Office of Civilian Radioactive Waste Management is working on cooperative nuclear waste isolation technology development programs with the Organization for Economic Cooperation and Development/Nuclear Energy Agency (OECD/NEA), Canada's Atomic Energy of Canada, Limited (AECL), Sweden, Switzerland, and the Federal Republic of Germany. This paper describes recent technology results that have been obtained in DOE's foreign cooperative programs. Specific technology development studies are discussed for cooperative efforts with Canada, OECD/NEA, and a natural analog project in Brazil

  16. Development and implementation of a high-throughput compound screening assay for targeting disrupted ER calcium homeostasis in Alzheimer's disease.

    Directory of Open Access Journals (Sweden)

    Kamran Honarnejad

    Full Text Available Disrupted intracellular calcium homeostasis is believed to occur early in the cascade of events leading to Alzheimer's disease (AD pathology. Particularly familial AD mutations linked to Presenilins result in exaggerated agonist-evoked calcium release from endoplasmic reticulum (ER. Here we report the development of a fully automated high-throughput calcium imaging assay utilizing a genetically-encoded FRET-based calcium indicator at single cell resolution for compound screening. The established high-throughput screening assay offers several advantages over conventional high-throughput calcium imaging technologies. We employed this assay for drug discovery in AD by screening compound libraries consisting of over 20,000 small molecules followed by structure-activity-relationship analysis. This led to the identification of Bepridil, a calcium channel antagonist drug in addition to four further lead structures capable of normalizing the potentiated FAD-PS1-induced calcium release from ER. Interestingly, it has recently been reported that Bepridil can reduce Aβ production by lowering BACE1 activity. Indeed, we also detected lowered Aβ, increased sAPPα and decreased sAPPβ fragment levels upon Bepridil treatment. The latter findings suggest that Bepridil may provide a multifactorial therapeutic modality for AD by simultaneously addressing multiple aspects of the disease.

  17. Development and Implementation of a High-Throughput Compound Screening Assay for Targeting Disrupted ER Calcium Homeostasis in Alzheimer's Disease

    Science.gov (United States)

    Honarnejad, Kamran; Daschner, Alexander; Giese, Armin; Zall, Andrea; Schmidt, Boris; Szybinska, Aleksandra; Kuznicki, Jacek; Herms, Jochen

    2013-01-01

    Disrupted intracellular calcium homeostasis is believed to occur early in the cascade of events leading to Alzheimer's disease (AD) pathology. Particularly familial AD mutations linked to Presenilins result in exaggerated agonist-evoked calcium release from endoplasmic reticulum (ER). Here we report the development of a fully automated high-throughput calcium imaging assay utilizing a genetically-encoded FRET-based calcium indicator at single cell resolution for compound screening. The established high-throughput screening assay offers several advantages over conventional high-throughput calcium imaging technologies. We employed this assay for drug discovery in AD by screening compound libraries consisting of over 20,000 small molecules followed by structure-activity-relationship analysis. This led to the identification of Bepridil, a calcium channel antagonist drug in addition to four further lead structures capable of normalizing the potentiated FAD-PS1-induced calcium release from ER. Interestingly, it has recently been reported that Bepridil can reduce Aβ production by lowering BACE1 activity. Indeed, we also detected lowered Aβ, increased sAPPα and decreased sAPPβ fragment levels upon Bepridil treatment. The latter findings suggest that Bepridil may provide a multifactorial therapeutic modality for AD by simultaneously addressing multiple aspects of the disease. PMID:24260442

  18. Advanced technology development reducing CO2 emissions

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Sup

    2010-09-15

    Responding to Korean government policies on green growth and global energy/ environmental challenges, SK energy has been developing new technologies to reduce CO2 emissions by 1) CO2 capture and utilization, 2) efficiency improvement, and 3) Li-ion batteries. The paper introduces three advanced technologies developed by SK energy; GreenPol, ACO, and Li-ion battery. Contributing to company vision, a more energy and less CO2, the three technologies are characterized as follows. GreenPol utilizes CO2 as a feedstock for making polymer. Advanced Catalytic Olefin (ACO) reduces CO2 emission by 20% and increase olefin production by 17%. Li-ion Batteries for automotive industries improves CO2 emission.

  19. Developing innovative environmental technologies for DOE needs

    International Nuclear Information System (INIS)

    Devgun, J.S.; Sewell, I.O.; DeGregory, J.

    1995-01-01

    Environmental restoration and waste management activities at US Department of Energy (DOE) facilities are diverse and complex. Contamination at DOE sites and facilities includes radionuclides, chlorinated hydrocarbons, volatile organic compounds, non-aqueous phase liquids, and heavy metals, among others. Soil and groundwater contamination are major areas of concern and DOE has focused very significant efforts in these areas. Relevant technology development activities are being conducted at DOE's own national laboratories, as well as through collaborative efforts with other federal agencies and the private sector. These activities span research and development (R ampersand D) of new concepts and techniques to demonstration and commercialization of mature technologies. Since 1990, DOE has also supported R ampersand D of innovative technologies through interagency agreements with US Environmental Protection Agency (EPA), US Department of Defense, the National Science Foundation, and others

  20. 78 FR 17418 - Rural Health Information Technology Network Development Grant

    Science.gov (United States)

    2013-03-21

    ... Information Technology Network Development Grant AGENCY: Health Resources and Services Administration (HRSA...-competitive replacement award under the Rural Health Information Technology Network Development Grant (RHITND... relinquishing its fiduciary responsibilities for the Rural Health Information Technology Network Development...

  1. Cosmic Origins (COR) Technology Development Program Overview

    Science.gov (United States)

    Werneth, Russell; Pham, B.; Clampin, M.

    2014-01-01

    The Cosmic Origins (COR) Program Office was established in FY11 and resides at the NASA Goddard Space Flight Center (GSFC). The office serves as the implementation arm for the Astrophysics Division at NASA Headquarters for COR Program related matters. We present an overview of the Program’s technology management activities and the Program’s technology development portfolio. We discuss the process for addressing community-provided technology needs and the Technology Management Board (TMB)-vetted prioritization and investment recommendations. This process improves the transparency and relevance of technology investments, provides the community a voice in the process, and leverages the technology investments of external organizations by defining a need and a customer. Goals for the COR Program envisioned by the National Research Council’s (NRC) “New Worlds, New Horizons in Astronomy and Astrophysics” (NWNH) Decadal Survey report includes a 4m-class UV/optical telescope that would conduct imaging and spectroscopy as a post-Hubble observatory with significantly improved sensitivity and capability, a near-term investigation of NASA participation in the Japanese Aerospace Exploration Agency/Institute of Space and Astronautical Science (JAXA/ISAS) Space Infrared Telescope for Cosmology and Astrophysics (SPICA) mission, and future Explorers.

  2. Smallholder agricultural technology development in Soroti district ...

    African Journals Online (AJOL)

    Mo

    Greater involvement of farmers in development and dissemination of agricultural technology is a key component in current reforms of ... its members to engage in demand-driven agricultural services in Soroti district and thereby contributed to the success of .... long training in integrated production and pest management.

  3. Theorizing about technological development of manufacturing companies

    DEFF Research Database (Denmark)

    Matthiesen, Rikke Vestergaard

    2012-01-01

    The aim of the paper is to apply metatriangulation and theory building to strengthen our theorizing on technological development of manufacturing firms. AMT literature is reviewed and by means of bracketing, the paper identifies a set of problematic assumptions embedded within traditional AMT res...

  4. Information and Communication Technologies for Development in ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    This project seeks to foster an inclusive knowledge society by building capacity to apply information and communication technology (ICT) to human, social and economic development in the Middle East, specifically Jordan, Lebanon, Palestine (West Bank and Gaza), Syria and Yemen. The project will focus on the areas of ...

  5. Mobile Haptic Technology Development through Artistic Exploration

    DEFF Research Database (Denmark)

    Cuartielles, David; Göransson, Andreas; Olsson, Tony

    2012-01-01

    This paper investigates how artistic explorations can be useful for the development of mobile haptic technology. It presents an alternative framework of design for wearable haptics that contributes to the building of haptic communities outside specialized research contexts. The paper also present...

  6. Developing Pre-service Teachers' Technology Integration ...

    African Journals Online (AJOL)

    Developing Pre-service Teachers' Technology Integration Competencies in Science and Mathematics Teaching: Experiences from Tanzania and Uganda. ... This study investigated the ICT integration practices in pre-service teacher education in the School of Education at Makerere University (College of Education and ...

  7. Constructive Technology Assessment for HIT development

    DEFF Research Database (Denmark)

    Høstgaard, Anna Marie Balling; Bertelsen, Pernille; Petersen, Lone Stub

    2013-01-01

    Experience and time has shown a need for new evaluation methods for evaluating Health Information Technology (HIT), as summative evaluation methods fail to accommodate the rapid and constant changes in HIT over time and to involve end-users, which has been recognized as an important success facto...... during all the phases in the process. Thereby anumber of problems were prevented to occur later on.Thus, the CTA method and its framework are useful for evaluators and project-management in order to facilitate and support successful HIT development.......Experience and time has shown a need for new evaluation methods for evaluating Health Information Technology (HIT), as summative evaluation methods fail to accommodate the rapid and constant changes in HIT over time and to involve end-users, which has been recognized as an important success factor...... in HIT development. A new evaluation methodology, including an analytical framework, has been developed specifically for HIT development: Constructive Technology Assessment (CTA) for HIT. It offers solutions to both the problems associated with summative technology evaluation and a way to involve end...

  8. Current Developments in DETER Cybersecurity Testbed Technology

    Science.gov (United States)

    2015-12-08

    1 Current Developments in DETER Cybersecurity Testbed Technology Terry Benzel1*, Bob Braden*, Ted Faber*, Jelena Mirkovic*, Steve Schwab...fundamentally transformational cybersecurity research methodologies. This paper discusses underlying rationale, together with initial design and...implementation, of key technical concepts that drive these transformations. 1. Introduction The DETER cybersecurity testbed [3] is a dedicated

  9. Strategic alliances in engineering, technology and development

    International Nuclear Information System (INIS)

    Jazrawi, W.

    1991-01-01

    The role of strategic alliances in the development of heavy oil resources, both mineable and in-situ, is discussed. A strategic alliance is defined as a custom designed, long term collaborative working arrangement between two parties to pool, exchange, and integrate their resources to maximize mutual gain. A combination of one or more of the following success factors is seen as contributing to the unlocking of static heavy oil resources: sufficiently high and sustained crude oil prices; strategic intent to pursue heavy oil development regardless of short-term setbacks or economic downturns; technology breakthroughs that can reduce bitumen supply and upgrading costs; and strategic alliances. An idealized model for strategic alliances designed to help develop heavy oil resources is illustrated. The advantages and pitfalls involved in strategic alliances are listed along with the characteristics of viable contract agreements for such alliances. Some examples of strategic alliances in engineering and technology development are presented from Alberta experience. 2 figs., 1 tab

  10. NASA Astrophysics Funds Strategic Technology Development

    Science.gov (United States)

    Seery, Bernard D.; Ganel, Opher; Pham, Bruce

    2016-01-01

    The COR and PCOS Program Offices (POs) reside at the NASA Goddard Space Flight Center (GSFC), serving as the NASA Astrophysics Division's implementation arm for matters relating to the two programs. One aspect of the PO's activities is managing the COR and PCOS Strategic Astrophysics Technology (SAT) program, helping mature technologies to enable and enhance future astrophysics missions. For example, the SAT program is expected to fund key technology developments needed to close gaps identified by Science and Technology Definition Teams (STDTs) planned to study several large mission concept studies in preparation for the 2020 Decadal Survey.The POs are guided by the National Research Council's "New Worlds, New Horizons in Astronomy and Astrophysics" Decadal Survey report, NASA's Astrophysics Implementation Plan, and the visionary Astrophysics Roadmap, "Enduring Quests, Daring Visions." Strategic goals include dark energy, gravitational waves, and X-ray observatories. Future missions pursuing these goals include, e.g., US participation in ESA's Euclid, Athena, and L3 missions; Inflation probe; and a large UV/Optical/IR (LUVOIR) telescope.To date, 65 COR and 71 PCOS SAT proposals have been received, of which 15 COR and 22 PCOS projects were funded. Notable successes include maturation of a new far-IR detector, later adopted by the SOFIA HAWC instrument; maturation of the H4RG near-IR detector, adopted by WFIRST; development of an antenna-coupled transition-edge superconducting bolometer, a technology deployed by BICEP2/BICEP3/Keck to measure polarization in the CMB signal; advanced UV reflective coatings implemented on the optics of GOLD and ICON, two heliophysics Explorers; and finally, the REXIS instrument on OSIRIS-REx is incorporating CCDs with directly deposited optical blocking filters developed by another SAT-funded project.We discuss our technology development process, with community input and strategic prioritization informing calls for SAT proposals and

  11. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus▿

    Science.gov (United States)

    Bista, Bipin Raj; Ishwad, Chandra; Wadowsky, Robert M.; Manna, Pradip; Randhawa, Parmjeet Singh; Gupta, Gaurav; Adhikari, Meena; Tyagi, Rakhi; Gasper, Gina; Vats, Abhay

    2007-01-01

    Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for “point of care” screening of BKV. PMID:17314224

  12. Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus.

    Science.gov (United States)

    Bista, Bipin Raj; Ishwad, Chandra; Wadowsky, Robert M; Manna, Pradip; Randhawa, Parmjeet Singh; Gupta, Gaurav; Adhikari, Meena; Tyagi, Rakhi; Gasper, Gina; Vats, Abhay

    2007-05-01

    Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for "point of care" screening of BKV.

  13. Multiplex polymerase chain reaction assay developed to diagnose adult bacterial meningitis in Taiwan.

    Science.gov (United States)

    Lee, Chi-Tsung; Hsiao, Kuang-Ming; Chen, Jin-Cherng; Su, Cheng-Chuan

    2015-11-01

    Acute bacterial meningitis causes high morbidity and mortality; the associated clinical symptoms often are insensitive or non-specific; and the pathogenic bacteria are geographically diverse. Clinical diagnosis requires a rapid and accurate methodology. This study aimed to develop a new multiplex polymerase chain reaction (mPCR) assay to detect simultaneously six major bacteria that cause adult bacterial meningitis in Taiwan: Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus aureus, Escherichia coli, and Acinetobacter baumannii. Species-specific primers for the six bacteria were developed using reference strains. The specificities of the mPCRs for these bacteria were validated, and the sensitivities were evaluated via serial dilutions. The mPCR assay specifically detected all of the six pathogens, particularly with sensitivities of 12 colony forming units (CFU)/mL, 90 CFU/mL, and 390 CFU/mL for E. coli, S. pneumoniae, and K. pneumoniae, respectively. This mPCR assay is a rapid and specific tool to detect the six major bacterial pathogens that cause acute adult meningitis in Taiwan, particularly sensitive for detecting E. coli, S. pneumoniae, and K. pneumoniae. The assay may facilitate early diagnosis and guidance for antimicrobial therapy for adult patients with this deadly disease in Taiwan. © 2015 APMIS. Published by John Wiley & Sons Ltd.

  14. Development And Application of Functional Assays For Freshwater Dissolved Organic Matter

    Science.gov (United States)

    Thacker, S.; Tipping, E.; Gondar, D.; Baker, A.

    2006-12-01

    Dissolved organic matter (DOM) in natural waters participates in many important ecological and geochemical reactions, including acid-base buffering, light absorption, proton binding, binding of heavy metals, organic contaminants, aluminium and radionuclides, adsorption at surfaces, aggregation and photochemical reactivity. We are studying DOM in order to understand and quantify these functional properties, so we can use the knowledge to predict the influence of DOM on the natural freshwater environment. As DOM has no readily identifiable structure, our approach is to measure what it does, rather than what it is. Thus, we have developed a series of 12 standardised, reproducible assays of physico-chemical functions of dissolved organic matter (DOM) in freshwaters. The assays provide quantitative information on light absorption, fluorescence, photochemical fading, pH buffering, copper binding, benzo(a)pyrene binding, hydrophilicity and adsorption to alumina. We have collected twenty DOM samples in total, ten samples from a eutrophic lake (Esthwaite Water) and ten samples from three stream waters. A mild isolation method was then used to concentrate the DOM samples for the assay work. When assaying the concentrates, parallel assays were also preformed with Suwannee River Fulvic Acid (SRFA), as a quality control standard. Our results showed that; (i) for eleven of the assays, the variability among the twenty DOM samples was significantly (p<0.001) greater than can be explained by analytical error, i.e. by comparison with results from the SRFA quality control; (ii) the functional properties of the DOM from Esthwaite Water are strongly influenced by the seasonally-dependent input of autochthonous DOM, derived from phytoplankton. The autochthonous DOM is less fluorescent, light absorbing, hydrophobic and has a lower acid group content and capacity to be adsorbed onto alumina than terrestrially derived allochthonous DOM; (iii) significant correlations were found between

  15. Green technology innovation in a developing country

    Science.gov (United States)

    Treesubsuntorn, Chairat; Dolphen, Rujira; Dhurakit, Prapai; Siswanto, Dian; Thiravetyan, Paitip

    2017-11-01

    Developing countries rapidly grow when green technology, which is referred to as eco-friendly processes or methods, is developed in parallel. Here, some examples of green technology research and development in Thailand will be overviewed. A huge amount of agricultural waste is generated during agricultural processes. Applying these agricultural wastes in order to maximize the benefits for environmental cleanups of water, soil and air has been studied and commercialized. For example: 1) Application of agricultural waste and/or biochar developed from agricultural waste as biological adsorbents for wastewater treatment in some industries, such as textile/dye industries, and printing industries. In addition, this agricultural waste can also be applied in decolorization of sugar syrup from sugar industries; 2) The research on modified biomaterials as adsorbents and packing materials in biofilters would also be presented, and now, pilot scale biofilters have been developed and applied to solve air pollution problems in the field for future commercialization; 3) Some agricultural waste and/or biochar developed from agricultural waste in our laboratory can promote rice growth and improve rice quality via the reduction of Cd uptake and translocation in rice. Phytoremediation technology, in which plants are used to improve the environmental quality in water and air, has also been studied and would be presented. 1) Some species of native Thai plants can effectively remove heavy metals and dye from wastewater. For this research, a constructed wetland for wastewater treatment was developed and applied in a real contaminated site. 2) In air phytoremediation, some plant species harbor highly volatile organic compound (VOC) removal efficiency. In addition, plants do not only absorb organic pollutants, but also they have the innate ability to degrade organic compounds and use them as carbon sources for their growth. In addition, plant growth-promoting (PGP) bacteria inoculation

  16. Development of fuel and energy storage technologies

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    Development of fuel cell power plants is intended of high-efficiency power generation using such fuels with less air pollution as natural gas, methanol and coal gas. The closest to commercialization is phosphoric acid fuel cells, and the high in efficiency and rich in fuel diversity is molten carbonate fuel cells. The development is intended to cover a wide scope from solid electrolyte fuel cells to solid polymer electrolyte fuel cells. For new battery power storage systems, development is focused on discrete battery energy storage technologies of fixed type and mobile type (such as electric vehicles). The ceramic gas turbine technology development is purposed for improving thermal efficiency and reducing pollutants. Small-scale gas turbines for cogeneration will also be developed. Development of superconduction power application technologies is intended to serve for efficient and stable power supply by dealing with capacity increase and increase in power distribution distance due to increase in power demand. In the operations to improve the spread and general promotion systems for electric vehicles, load leveling is expected by utilizing and storing nighttime electric power. Descriptions are given also on economical city systems which utilize wide-area energy. 30 figs., 7 tabs.

  17. Technology development risk assessment and mixed interests

    International Nuclear Information System (INIS)

    Borrelli, G.; Sartori, S.

    1992-05-01

    The main purpose of this work is to demonstrate by means of a critical analysis of the state-of-the-art in technological and environmental risk analysis and decision making, that risk and environmental management decisions involve heterogeneous groups of social actors, each representing conflicting interests. It is argued that risk analyses should therefore be based on social interaction and communication paradigma, as well as, on a new rational way of thinking concerning the optimum choice of suitable technological development strategies leading towards a publicly acceptable balance between national energy-economic strategic necessities and social and individual perception of risk

  18. Survey and analysis of federally developed technology

    Energy Technology Data Exchange (ETDEWEB)

    Reed, J.E.; Conrad, J.L.

    1983-02-01

    The methodology and results of a test effort to determine whether there exist unexpected opportunities for the direct transfer of technologies from federal laboratories to industry are presented. Specifically, the latest results of six federal laboratories with potential application in the pulp and paper industry, particularly those results applicable to improving energy productivity, were evaluated, cataloged, and distributed to industry representatives to gauge their reaction. The principal methodological steps in this effort were the development of a taxonomy of the pulp and paper industry, identification of industry needs and laboratory capabilities, laboratory visits, review of technology findings with industry, and evaluation and compilation of industry responses.

  19. Continuation of Crosscutting Technology Development at Cast

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Roe-Hoan

    2012-03-31

    This Final Technical Report describes progress made on the sub-projects awarded in the Cooperative Agreement DE-FC26-05NT42457: Continuation of Crosscutting Technology Development at Center for Advanced Separation Technologies (CAST). The final reports for each sub-project are attached in the appendix. Much of the research to be conducted with Cooperative Agreement funds will be longer-term, high-risk, basic research and will be carried out in five broad areas: a) Solid-solid separation b) Solid-liquid separation c) Chemical/Biological Extraction d) Modeling and Control, and e) Environmental Control.

  20. New weapons to penetrate the armor: Novel reagents and assays developed at the NCI RAS Initiative to enable discovery of RAS therapeutics.

    Science.gov (United States)

    Esposito, Dominic; Stephen, Andrew G; Turbyville, Thomas J; Holderfield, Matthew

    2018-02-09

    Development of therapeutic strategies against RAS-driven cancers has been challenging due in part to a lack of understanding of the biology of the system and the ability to design appropriate assays and reagents for targeted drug discovery efforts. Recent developments in the field have opened up new avenues for exploration both through advances in the number and quality of reagents as well as the introduction of novel biochemical and cell-based assay technologies which can be used for high-throughput screening of compound libraries. The reagents and assays developed at the NCI RAS Initiative offer a suite of new weapons that could potentially be used to enable the next generation of RAS drug discovery efforts with the hope of finding novel therapeutics for a target once deemed undruggable. Copyright © 2018. Published by Elsevier Ltd.

  1. Technologies in support of CANDU development

    International Nuclear Information System (INIS)

    Turner, C.; Tapping, B.

    2005-01-01

    Atomic Energy of Canada, Ltd. (AECL) has significant research and development (R and D) programs designed to meet the needs of both existing CANDU reactors and new and evolving CANDU plant designs. These R and D programs cover a wide range of technology, from chemistry and materials support through to inspection and life management tools. Emphasis is placed on effective technology development programs for fuel channels, feeders and steam generators to ensure their operation through design life, and beyond. This paper specifically addresses how the R and D has been applied in the production of longer-lived pressure tubes for the most recent CANDU 6 reactors, and how this technology forms the basis for the pressure tubes of the Advanced CANDU Reactor (ACR). Similarly, AECL has developed solutions for other critical components such as calandria tubes, feeder pipe and steam generators. The paper also discusses how the R and D knowledge has been integrated into aging management databases and health monitoring tools. Since 1997, AECL has been working with CANDU utilities on comprehensive and integrated CANDU Plant Life Management (PLiM) programs for successful and reliable plant operation through design life and beyond. AECL has developed and implemented an advanced chemistry monitoring and diagnostic system, called ChemAND which allows on-line access by the operators to current and past chemistry conditions enabling appropriate responses and facilitating planning of shutdown maintenance actions. An equivalent tool for monitoring, trending and diagnosing thermal and mechanical data has also been developed; this tool is called ThermAND. AECL is developing the Maintenance Information, Monitoring, and Control (MIMC) system, which provide information to the user for condition-based decision-making in maintenance. To enable more effective inspections, surveillance and data collection, AECL has developed unique one-off tooling to carry out unanticipated inspection and repair

  2. Development of safety analysis technology for LMR

    International Nuclear Information System (INIS)

    Hahn, Do Hee; Kwon, Y. M.; Suk, S. D.

    2002-05-01

    In the present study, the KALIMER safety analysis has been made for the transients considered in the design concept, hypothetical core disruptive accident (HCDA), and containment performance with the establishment of the design basis. Such analyses have not been possible without the computer code improvement, and the experience attained during this research period must have greatly contributed to the achievement of the self reliance in the domestic technology establishment on the safety analysis areas of the conceptual design. The safety analysis codes have been improved to extend their applicable ranges for detailed conceptual design, and a basic computer code system has been established for HCDA analysis. A code-to-code comparison analysis has been performed as a part of code verification attempt, and the leading edge technology of JNC also has been brought for the technology upgrade. In addition, the research and development on the area of the database establishment has been made for the efficient and systematic project implementation of the conceptual design, through performances on the development of a project scheduling management, integration of the individually developed technology, establishment of the product database, and so on, taking into account coupling of the activities conducted in each specific area

  3. PRINTING TECHNIQUES: RECENT DEVELOPMENTS IN PHARMACEUTICAL TECHNOLOGY.

    Science.gov (United States)

    Jamroz, Witold; Kurek, Mateusz; Lyszczarz, Ewelina; Brniak, Witold; Jachowicz, Renata

    2017-05-01

    In the last few years there has been a huge progress in a development of printing techniques and their application in pharmaceutical sciences and particularly in the pharmaceutical technology. The variety of printing methods makes it necessary to systemize them, explain the principles of operation, and specify the possibilities of their use in pharmaceutical technology. This paper aims to review the printing techniques used in a drug development process. The growing interest in 2D and 3D printing methods results in continuously increasing number of scientific papers. Introduction of the first printed drug Spritam@ to the market seems to be a milestone of the 3D printing development. Thus, a particular aim of this review is to show the latest achievements of the researchers in the field of the printing medicines.

  4. Application of radiation technology in vaccines development.

    Science.gov (United States)

    Seo, Ho Seong

    2015-07-01

    One of the earliest methods used in the manufacture of stable and safe vaccines is the use of chemical and physical treatments to produce inactivated forms of pathogens. Although these types of vaccines have been successful in eliciting specific humoral immune responses to pathogen-associated immunogens, there is a large demand for the development of fast, safe, and effective vaccine manufacturing strategies. Radiation sterilization has been used to develop a variety of vaccine types, because it can eradicate chemical contaminants and penetrate pathogens to destroy nucleic acids without damaging the pathogen surface antigens. Nevertheless, irradiated vaccines have not widely been used at an industrial level because of difficulties obtaining the necessary equipment. Recent successful clinical trials of irradiated vaccines against pathogens and tumors have led to a reevaluation of radiation technology as an alternative method to produce vaccines. In the present article, we review the challenges associated with creating irradiated vaccines and discuss potential strategies for developing vaccines using radiation technology.

  5. Decommissioning Technology Development for Nuclear Research Facilities

    International Nuclear Information System (INIS)

    Lee, K. W.; Kang, Y. A.; Kim, G. H.

    2007-06-01

    It is predicted that the decommissioning of a nuclear power plant would happen in Korea since 2020 but the need of partial decommissioning and decontamination for periodic inspection and life extension still has been on an increasing trend and its domestic market has gradually been extended. Therefore, in this project we developed following several essential technologies as a decommissioning R and D. The measurement technology for in-pipe radioactive contamination was developed for measuring alpha/beta/gamma emitting nuclides simultaneously inside a in-pipe and it was tested into the liquid waste transfer pipe in KRR-2. And the digital mock-up system for KRR-1 and 2 was developed for choosing the best scenarios among several scenarios on the basis of various decommissioning information(schedule, waste volume, cost, etc.) that are from the DMU and the methodology of decommissioning cost estimation was also developed for estimating a research reactor's decommissioning cost and the DMU and the decommissioning cost estimation system were incorporated into the decommissioning information integrated management system. Finally the treatment and management technology of the irradiated graphites that happened after decommissioning KRR-2 was developed in order to treat and manage the irradiated graphites safely

  6. Latest Developments in Nuclear Emulsion Technology

    Science.gov (United States)

    Morishima, Kunihiro

    Nuclear emulsion is high sensitive photographic film used for detection of three-dimensional trajectory of charged particles. These trajectories are recorded as tracks consist of a lot of silver grains. The size of silver grain is about 1 μm, so that nuclear emulsion has submicron three-dimensional spatial resolution, which gives us a few mrad three-dimensional angular resolution. The important technical progress was speed-up of the read-out technique of nuclear emulsions built with optical microscope system. We succeeded in developing a high-speed three-dimensional read-out system named Super Ultra Track Selector (S-UTS) with the operating read-out speed of approximately 50 cm2/h. Nowadays we are developing the nuclear emulsion gel independently in Nagoya University by introducing emulsion gel production machine. Moreover, we are developing nuclear emulsion production technologies (gel production, poring and mass production). In this paper, development of nuclear emulsion technologies for the OPERA experiment, applications by the technologies and current development are described.

  7. Decommissioning Technology Development for Nuclear Research Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Lee, K. W.; Kang, Y. A.; Kim, G. H. (and others)

    2007-06-15

    It is predicted that the decommissioning of a nuclear power plant would happen in Korea since 2020 but the need of partial decommissioning and decontamination for periodic inspection and life extension still has been on an increasing trend and its domestic market has gradually been extended. Therefore, in this project we developed following several essential technologies as a decommissioning R and D. The measurement technology for in-pipe radioactive contamination was developed for measuring alpha/beta/gamma emitting nuclides simultaneously inside a in-pipe and it was tested into the liquid waste transfer pipe in KRR-2. And the digital mock-up system for KRR-1 and 2 was developed for choosing the best scenarios among several scenarios on the basis of various decommissioning information(schedule, waste volume, cost, etc.) that are from the DMU and the methodology of decommissioning cost estimation was also developed for estimating a research reactor's decommissioning cost and the DMU and the decommissioning cost estimation system were incorporated into the decommissioning information integrated management system. Finally the treatment and management technology of the irradiated graphites that happened after decommissioning KRR-2 was developed in order to treat and manage the irradiated graphites safely.

  8. Development of laser technology in Poland

    CERN Document Server

    Gajda, J

    2009-01-01

    The paper presents chosen development threads of laser technology and associated branches of optoelectronics in this country. An occasion to summarize the work and show their current status is the 50 th anniversary of construction of the first laser. The first laser in Poland was launched successfully in 1969, almost simultaneously at WAT and PW. Domestic achievements in this area are summarized every three years by Symposium on Laser Technology held traditionally in Swinoujscie. The work carried on in Poland concerns technology of laser materials, construction of new lasers and associated equipment as well as laser applications. Many technical teams participate in laser oriented European structural and framework projects. Ths is an invited paper to present participation of Polish teams in such European projects as EuCARD - European Coordination of Accelerator Research, FLASH - Free ELctron Laser in DESY Hamburg, E-XFEL - European X-Ray FEL, ELI - Extreme Light Infrastructure, and HIPER.

  9. Space solar cell technology development - A perspective

    Science.gov (United States)

    Scott-Monck, J.

    1982-01-01

    The developmental history of photovoltaics is examined as a basis for predicting further advances to the year 2000. Transistor technology was the precursor of solar cell development. Terrestrial cells were modified for space through changes in geometry and size, as well as the use of Ag-Ti contacts and manufacture of a p-type base. The violet cell was produced for Comsat, and involved shallow junctions, new contacts, and an enhanced antireflection coating for better radiation tolerance. The driving force was the desire by private companies to reduce cost and weight for commercial satellite power supplies. Liquid phase epitaxial (LPE) GaAs cells are the latest advancement, having a 4 sq cm area and increased efficiency. GaAs cells are expected to be flight ready in the 1980s. Testing is still necessary to verify production techniques and the resistance to electron and photon damage. Research will continue in CVD cell technology, new panel technology, and ultrathin Si cells.

  10. Human CCDC47 sandwich immunoassay development with electrochemiluminescence technology.

    Science.gov (United States)

    Wu, Wenfang S; Zhu, Liang; Patil, Saurabh; Gokul, Karthiga; Reilly, Sean; Chan, Joyce; Tekumalla, Poornima; Vishnudas, Vivek; Kiebish, Michael A; Sarangarajan, Rangaprasad; Akmaev, Viatcheslav R; Kellogg, Mark D; Narain, Niven R

    2018-01-01

    Coiled-Coil Domain Containing 47 (CCDC47) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. CCDC47 also interacts with ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief. In this report, we developed human CCDC47 monoclonal antibodies and a sandwich immunoassay for CCDC47 measurement in biological matrices. Specificity of developed antibodies were confirmed by immunoblot and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of immunoprecipitated cell lysates. To achieve high analytical sensitivity, traditional colorimetric enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence (ECL) technology were compared, and 3 logs of increased sensitivity was observed with the use of ECL. A CCDC47 sandwich ECL assay was subsequently developed and performances evaluated for calibration curves, precision and accuracy, as well as selectivity and interferences for sample measurement. Sample stability was also characterized for freeze/thaw cycles and short/long term storage conditions. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Development of technology for biological dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Kim, In Gyu; Kim, Kug Chan; Lee, Kang Sik; Kim, Jin Kyu; Chun, Ki Jung; Shim, Hye Won; Park, Seon Young

    1997-07-01

    Adult male rats were treated a single, whole body exposure to a dose of 0.1, 0.5, 1, 2, 3, 5, 7 and 9 Gy. The animal were sacrificed 6, 24, 48, 72, 96 and 212 hours following exposure. Enzyme activity changes such as alkaline phosphatase, GOT, creating kinase, MDH and LDH in rat serum as biochemical indicators useful for evaluating radiation exposure were measured. An competitive enzyme linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum after gamma-irradiation. Rat alpha-2 macroglobulin, alpha-1 acid glycoprotein, haptoglobin, ceruloplasmin, C-reactive protein and alpha-1 antitrypsin were purified from the plasma of turpentine treated rats. In vitro somatic mutation induced by gamma-irradiation and pentachlorophenol (PCP) which is representative of chemical pollutant was measured at the hypoxanthine-guanine phosphorybosyl transferase (HPRT) locus in human T-lymphocytes by a cell cloning assay. Mutant cells were selected by their ability to form a clone in the presence of purine analogue 6-thioguanine. Reverse transcriptase/polymerase chain reaction technique was needed for the mutation spectrum to discriminate combined exposure to radiation and chemicals. (author). 98 refs., 7 tabs., 47 figs.

  12. Technology development for nuclear material accountability

    International Nuclear Information System (INIS)

    Hong, Jong Sook; Lee, Byung Doo; Cha, Hong Ryul; Choi, Hyoung Nae; Park, Ho Jun.

    1990-01-01

    Neutron yields from 19 F(α,n) 22 Na reaction of uranium neutron interaction with uranium-bass materials, and the characteristics of shielded neutron assay probe have been studied. On the basis of the above examination, U-235 enrichment in UF 6 cylinders like model 30B and model 48Y was measured by the reaction and U-235 contents in the containers by non-destructive total passive neutron assay method. Total measurement efficiency as a result was found to be 6.44 x 10 -4 and 1.25 x 10 -4 for model 30B and model 40Y UF 6 cylinder, respectively. The uncertainty of measured enrichment as compared to Tag value obtained from chemical analysis approached about 5 % of relative error at 95 % confidence interval. In the follow-up action for the previously developed (1988) computer system of nuclear material accounting the error searching and treatment routine in accordance with code 10, of IAEA and respective facility attachment has been added to easing the burden of manual error correction by operator. In addition, the procedure for LEMUF calculation has been prepared to help bulk facility operators evaluating MUF in the period of material balance. (author)

  13. Development of an in Vitro Potency Assay for Anti-anthrax Lethal Toxin Neutralizing Antibodies

    Directory of Open Access Journals (Sweden)

    Sjoerd Rijpkema

    2012-01-01

    Full Text Available Lethal toxin (LT of Bacillus anthracis reduces the production of a number of inflammatory mediators, including transcription factors, chemokines and cytokines in various human cell lines, leading to down-regulation of the host inflammatory response. Previously we showed that the reduction of interleukin-8 (IL-8 is a sensitive marker of LT-mediated intoxication in human neutrophil-like NB-4 cells and that IL-8 levels are restored to normality when therapeutic monoclonal antibodies (mAb with toxin-neutralising (TN activity are added. We used this information to develop cell-based assays that examine the effects of TN therapeutic mAbs designed to treat LT intoxication and here we extend these findings. We present an in vitro assay based on human endothelial cell line HUVEC jr2, which measures the TN activity of therapeutic anti-LT mAbs using IL-8 as a marker for intoxication. HUVEC jr2 cells have the advantage over NB-4 cells that they are adherent, do not require a differentiation step and can be used in a microtitre plate format and therefore can facilitate high throughput analysis. This human cell-based assay provides a valid alternative to the mouse macrophage assay as it is a more biologically relevant model of the effects of toxin-neutralising antibodies in human infection.

  14. Development of the advanced CANDU technology

    Energy Technology Data Exchange (ETDEWEB)

    Suk, Soo Dong; Min, Byung Joo [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of); Na, Y. H.; Lee, S. Y.; Choi, J. H.; Lee, B. C.; Kim, S. N.; Jo, C. H.; Paik, J. S.; On, M. R.; Park, H. S.; Kim, S. R. [Korea Electric Power Co., Taejon (Korea, Republic of)

    1997-07-01

    The purpose of this study is to develop the advanced design technology to improve safety, operability and economy and to develop and advanced safety evaluation system. More realistic and reasonable methodology and modeling was employed to improve safety margin in containment analysis. Various efforts have been made to verify the CATHENA code which is the major safety analysis code for CANDU PHWR system. Fully computerized prototype ECCS was developed. The feasibility study and conceptual design of the distributed digital control system have been performed as well. The core characteristics of advanced fuel cycle, fuel management and power upgrade have been studied to determine the advanced core. (author). 77 refs., 51 tabs., 108 figs.

  15. Development of MOX manufacturing technology in BNFL

    International Nuclear Information System (INIS)

    Buchan, P.G.; Powell, D.J.; Edwards, J.

    1998-01-01

    BNFL is successfully operating a small scale MOX fuel fabrication facility at its Sellafield Site and is currently constructing an advanced, commercial scale MOX facility to complement its existing LWR UO 2 fabrication capability. BNFL's MOX fuel capability is fully supported by a comprehensive technology development programme aimed at providing a high quality product which is successfully competing in the market. Building on the experience gained over the last 30 years, is from the production of both thermal and fast reactor MOX fuels, BNFL's development team set a standard for its MOX product which is targeted at exceeding the performance of UO 2 fuel in reactor. In order to meet the stringent design requirements the product development team has introduced the Short Binderless Route (SBR) process that is now used routinely in BNFL's MOX Demonstration Facility (MDF) and which forms the basis for BNFL's large scale Sellafield MOX Plant. This plant not only uses the SBR process for MOX production but also incorporates the most advanced technology available anywhere in the world for nuclear fuel production. A detailed account of the technology developed by BNFL to support its MOX fuels business will be provided, together with an explanation of the processes and plants used for MOX fuel production by BNFL. The paper also looks at the future needs of the MOX business and how improvements in pellet design can assist the MOX fabrication production process to meet the user demand requirements of utilities around the world. (author)

  16. Development of germline manipulation technologies in livestock

    International Nuclear Information System (INIS)

    Whitelaw, C.B.A.

    2005-01-01

    Genetic improvement by conventional breeding is restricted to those genetic loci present in the parental breeding individuals. Gene addition through transgenic technology offers a route to overcome this restriction. The transgene can be introduced into the germ cells or the fertilized zygote, using viral vectors, by simple co-culture or direct micro-injection. Alternatively, the transgene can be incorporated into a somatic cell, which is then incorporated into a developing embryo. This latter approach allows gene-targeting strategies to be employed. Using pronuclear injection methods, transgenic livestock have been generated with the aim of enhancing breeding traits of agricultural importance, or for biomedical applications. Neither has been taken beyond the development phase. Before they are, in addition to issues of commercial development, basic technological issues addressing inefficiency and complexity of the methodology need to be overcome, and appropriate gene targets identified. At the moment, perhaps the most encouraging development involves the use of viral vectors that offer increased simplicity and efficiency. By combining this new technology with transgenes that evoke the powerful intracellular machinery involved in RNA interference, pioneering applications to generate animals that are less susceptible to infectious disease may be possible. (author)

  17. Development of neutron science and technology

    International Nuclear Information System (INIS)

    Lee, Ki Hong; Seong, Baik Seok; Lee, Jeong Soo

    2012-04-01

    Using various neutron scattering, imaging, and activation analysis instruments and irradiation facility and capsules, the short-term industrial application and mid and long-term basic science with neutrons was carried out. In this regard, we proposed the utilization of the neutron scattering and diffraction techniques to the study of physical, mechanical material properties in industrial components. The nano magnetic thin film structure study using neutron reflectometry, spin structure and dynamics study using neutron scattering, hydrogen combination structure study using single crystal diffraction were carried out. The triple-axis spectrometer has been installed. Also, a new growth facility of single crystal has been developed to supply crystals for the neutron scattering experiment. We have contributed to the performance enhancement of hydrogen fuel cell by the development of quantitative neutron radiography technology and developed the differential phase imaging technology using silicon grating. To perform precise neutron activation analysis, a Compton suppressed gamma-ray spectroscopy system was installed. Through the analysis of actual samples as well as geological and biological reference materials, performance test was carried out. We built up analytical data base and develope integrated analytical program for INAA/PGAA. The analysis and evaluation technology of the irradiation capsule test in HANARO for the commercial and future nuclear reactor systems was improved

  18. Development of Advanced Ceramic Manufacturing Technology; FINAL

    International Nuclear Information System (INIS)

    Pujari, V.K.

    2001-01-01

    Advanced structural ceramics are enabling materials for new transportation engine systems that have the potential for significantly reducing energy consumption and pollution in automobiles and heavy vehicles. Ceramic component reliability and performance have been demonstrated in previous U.S. DOE initiatives, but high manufacturing cost was recognized as a major barrier to commercialization. Norton Advanced Ceramics (NAC), a division of Saint-Gobain Industrial Ceramics, Inc. (SGIC), was selected to perform a major Advanced Ceramics Manufacturing Technology (ACMT) Program. The overall objectives of NAC's program were to design, develop, and demonstrate advanced manufacturing technology for the production of ceramic exhaust valves for diesel engines. The specific objectives were (1) to reduce the manufacturing cost by an order of magnitude, (2) to develop and demonstrate process capability and reproducibility, and (3) to validate ceramic valve performance, durability, and reliability. I n order to achieve these objectives, NAC, a leading U.S. advanced ceramics component manufacturer, assembled a multidisciplinary, vertically integrated team. This team included: a major diesel engine builder, Detroit Diesel Corporation (DDC); a corporate ceramics research division, SGIC's Northboro R and D Center; intelligent processing system developers, BDM Federal/MATSYS; a furnace equipment company, Centorr/Vacuum Industries; a sintering expert, Wittmer Consultants; a production OEM, Deco-Grand; a wheel manufacturer and grinding operation developer, Norton Company's Higgins Grinding Technology Center (HGTC); a ceramic machine shop, Chand Kare Technical Ceramics; and a manufacturing cost consultant, IBIS Associates. The program was divided into four major tasks: Component Design and Specification, Component Manufacturing Technology Development, Inspection and Testing, and Process Demonstration

  19. Development of safety analysis technology for LMR

    International Nuclear Information System (INIS)

    Lee, Y. B.; Kwon, Y. M.; Suk, S. D.

    2005-03-01

    The MATRA-LMR-FB has been developed internally for the damage prevention as well as the safety assessment during a channel blockage accident and, as a the result, the quality of the code becomes comparable to that developed in the leading countries. For a code-to-code comparison, KAERI could have access to the SASSYS-1 through a bilateral collaboration between KAERI and ANL. The study could bring into the reliability improvements both on the reactivity models in the SSC-K and on the SSC-K prediction capability. It finally leads to the completion of the SSC-K version 1.3 resulting from the qualitative and quantitative code-to-code comparison. The preliminary analysis for a metal fueled LMR could also become possible with the MELT-III and the VENUS-II, which had originally been developed for the HCDA analysis with an oxidized fuel, by developing the relevant models For the development of the safety evaluation technology, the safety limits have been set up, and the analyses of the internal and external channel blockages in an assembly have also been performed. Besides, the more reliable analysis results on the key design concepts could be obtained by way of the methodology improvement resulting from the qualitative and quantitative comparison study. For an efficient and systematic control of the main project, the integration of the developed technologies and the establishment of their data base have been pursued. It has gone through the development of the process control with taking account of interfaces among the sub-projects, the overall coordination of the developed technologies, the data base for the design products, and so on

  20. Office of Technology Development integrated program for development of in situ remediation technologies

    International Nuclear Information System (INIS)

    Peterson, M.

    1992-08-01

    The Department of Energy's Office of Technology Development has instituted an integrated program focused on development of in situ remediation technologies. The development of in situ remediation technologies will focus on five problem groups: buried waste, contaminated soils, contaminated groundwater, containerized wastes and underground detonation sites. The contaminants that will be included in the development program are volatile and non volatile organics, radionuclides, inorganics and highly explosive materials as well as mixtures of these contaminants. The In Situ Remediation Integrated Program (ISR IP) has defined the fiscal year 1993 research and development technology areas for focusing activities, and they are described in this paper. These R ampersand D topical areas include: nonbiological in situ treatment, in situ bioremediation, electrokinetics, and in situ containment

  1. Assay Development for the Discovery of Semaphorin 3B Inducing Agents from Natural Product Sources

    Science.gov (United States)

    Yong, Yeonjoong; Pan, Li; Ren, Yulin; Fatima, Nighat; Ahmed, Safia; Chang, Leng Chee; Zhang, Xiaoli; Kinghorn, A. Douglas; Swanson, Steven M.; Carcache de Blanco, Esperanza J.

    2014-01-01

    Semaphorins are a class of membrane-bound and secreted proteins. They have been found to regulate basic cell functions such as axonal growth cone guidance and recent studies have focused on their effect on tumor progression. Semaphorin 3B (Sema 3B) particularly is a secreted protein that has been known to modulate proliferation and apoptosis, processes that are critical for tumor progression and development. In spite of its importance, there is yet no high-throughput screening assay available to detect or quantify the expression of Sema 3B for natural product anticancer drug discovery purposes. Therefore, the development of a new high-throughput bioassay for the discovery of Sema 3B inducing agents from natural product sources is described herein. A wide variety of pure compounds and extracts from plants and microorganisms has been found suitable for screening using this Sema 3B assay to detect and quantify the effect of Sema 3B inducing agents and thereby identify new selective bioactive Sema 3B lead compounds for anticancer drug discovery and development. Also, this new bioassay procedure is based on a high-throughput platform using an enzyme-linked immunosorbent assay that involves the optimization of sensitivity and selectivity levels as well as accuracy, reproducibility, robustness, and cost effectiveness. PMID:25016954

  2. A mycobacterial growth inhibition assay (MGIA) for bovine TB vaccine development.

    Science.gov (United States)

    Pepponi, Ilaria; Khatri, Bhagwati; Tanner, Rachel; Villarreal-Ramos, Bernardo; Vordermeier, Martin; McShane, Helen

    2017-09-01

    Human tuberculosis remains a significant cause of mortality and morbidity throughout the world. The global economic impact of bovine TB is considerable. An effective vaccine would be the most cost-effective way to control both epidemics, particularly in emerging economies. TB vaccine research would benefit from the identification of an immune correlate of protection with which vaccines could be gated at both preclinical and clinical levels. In-vitro mycobacterial growth inhibition assays (MGIA) are functional assays that include most aspects of the complex host immune response to mycobacteria, and they may serve as functional immune correlates for vaccine development. We applied to cattle an MGIA that was developed for use with human and murine samples. Several technical difficulties were encountered while transferring it to the cattle model. However, our data demonstrate that the assay was not discriminatory in cattle and further work is needed before using it for bovine TB vaccine development. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  3. Development and Validation of a Semiquantitative, Multitarget PCR Assay for Diagnosis of Bacterial Vaginosis

    Science.gov (United States)

    Lembke, Bryndon D.; Ramachandran, Kalpana; Body, Barbara A.; Nye, Melinda B.; Rivers, Charles A.; Schwebke, Jane R.

    2012-01-01

    Quantitative PCR assays were developed for 4 organisms reported previously to be useful positive indicators for the diagnosis of bacterial vaginosis (BV)—Atopobium vaginae, Bacterial Vaginosis-Associated Bacterium 2 (BVAB-2), Gardnerella vaginalis, and Megasphaera-1—and a single organism (Lactobacillus crispatus) that has been implicated as a negative indicator for BV. Vaginal samples (n = 169), classified as positive (n = 108) or negative (n = 61) for BV based on a combination of the Nugent Gram stain score and Amsel clinical criteria, were analyzed for the presence and quantity of each of the marker organisms, and the results were used to construct a semiquantitative, multiplex PCR assay for BV based on detection of 3 positive indicator organisms (A. vaginae, BVAB-2, and Megasphaera-1) and classification of samples using a combinatorial scoring system. The prototype BV PCR assay was then used to analyze the 169-member developmental sample set and, in a prospective, blinded manner, an additional 227 BV-classified vaginal samples (110 BV-positive samples and 117 BV-negative samples). The BV PCR assay demonstrated a sensitivity of 96.7% (202/209), a specificity of 92.2% (153/166), a positive predictive value of 94.0%, and a negative predictive value of 95.6%, with 21 samples (5.3%) classified as indeterminate for BV. This assay provides a reproducible and objective means of evaluating critical components of the vaginal microflora in women with signs and symptoms of vaginitis and is comparable in diagnostic accuracy to the conventional gold standard for diagnosis of BV. PMID:22535982

  4. Technology Development for High Efficiency Optical Communications

    Science.gov (United States)

    Farr, William H.

    2012-01-01

    Deep space optical communications is a significantly more challenging operational domain than near Earth space optical communications, primarily due to effects resulting from the vastly increased range between transmitter and receiver. The NASA Game Changing Development Program Deep Space Optical Communications Project is developing four key technologies for the implementation of a high efficiency telecommunications system that will enable greater than 10X the data rate of a state-of-the-art deep space RF system (Ka-band) for similar transceiver mass and power burden on the spacecraft. These technologies are a low mass spacecraft disturbance isolation assembly, a flight qualified photon counting detector array, a high efficiency flight laser amplifier and a high efficiency photon counting detector array for the ground-based receiver.

  5. Data on development of new energy technologies

    Science.gov (United States)

    1994-03-01

    The paper compiles data on the trend of development of new energy technologies into a book. By category, renewable energy is solar energy, wind power generation, geothermal power generation, ocean energy, and biomass. As a category of fuel form conversion, cited are coal liquefaction/gasification, coal gasification combined cycle power generation, and natural gas liquefaction/decarbonization. The other categories are cogeneration by fuel cell and ceramic gas turbine, district heat supply system, power load leveling technology, transportation-use substitution-fuel vehicle, and others (Stirling engine, superconducting power generator, etc.). The data are systematically compiled on essential principles, transition of introduction, objectives of introduction, status of production, cost, development schedule, performance, etc. The paper also deals with the related legislation system, developmental organizations, and a menu for power companies' buying surplus power.

  6. The Plasma Hearth Process Technology Development Project

    International Nuclear Information System (INIS)

    Geimer, R.; Batdorf, J.; Wolfe, P.

    1993-01-01

    The US DOE Office of Technology Development (OTD) is currently evaluating the Plasma Hearth Process (PHP) for potential treatment of several DOE waste types. The PHP is a high-temperature vitrification process that has potential application for a wide range of mixed waste types in both the low-level and transuranic mixed waste categories. The PHP is being tested under both the OTD Mixed Waste Integrated Program and the Buried Waste Integrated Demonstration. Initial testing has been completed on several different surrogate waste forms that are representative of some of the DOE mixed waste streams. Destruction of organic material exceeds that of conventional incineration technologies. The vitrified residual has leaching characteristics comparable to glass formulations produced in the high-level waste program. The first phase of the PHP demonstration project has been successfully completed, and the project is currently beginning a comprehensive second phase of development and testing

  7. Development of Radiation Breeding Technology of Macroalgae

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Jongil; Park, J. H.; Song, B. S.; Kim, J. K.; Kim, J. H.; Lee, H. J.; Yang, H. Y.

    2013-01-15

    Macroalgae has been utilized as food material from long time ago, and recently newly recognized as functional materials and the source of bio-fuel. But, the study on the macroalgae is just beginning and the study on radiation effect and radiation breeding technology was not reported. In this study, the effect of radiation on the macroalgae Porphyra was investigated for the development of new mutant strains. Prphyra was successively cultivated in the laboratory chamber with optimized growth condition. Also, the radiation sensitivity of Porphyra was determined. To develop the mutant strain, irradiated Porphyra strains were screened for better growth and higher resistance against oxidative stress. The selected Porphyra was further cultivated in ocean site. Also, several genes from mutant Porphyra was heterologous expressed and studied for its dunctionality. This results can provide mutation technology of macroalgae and further contribute in the activation of fishery industry and national health enhancement.

  8. Thai health technology assessment guideline development.

    Science.gov (United States)

    Teerawattananon, Yot; Chaikledkaew, Usa

    2008-06-01

    Health Technology Assessment (HTA) is a comprehensive form of policy research that provides information on the consequences of the application of health technology. It is used primarily to guide health care resource allocation decisions. In Thailand, there is increasing impetus to use HTA information to allow more explicit and transparent health care priority setting. A previous study indicated that serious attention needed to be given to the quality of reporting and the use of information in the analyses. These problems could be reduced by setting up standard guidelines for conducting HTA to stimulate the provision of standardized, reliable and good quality information for policy makers. Nevertheless, Thailand has not yet set up such guidelines. This may lead to low quality evaluations. Therefore, the objective of this article was to describe the rationale for guideline development, supporting principles, guideline development process, sources of information, and future challenges for HTA.

  9. Development of the centribaric dewatering technology

    Energy Technology Data Exchange (ETDEWEB)

    Keles, S.; Luttrell, G.; Yoon, R.H.; Estes, T.; Schultz, W.; Bethell, P. [Virginia Tech, Blacksburg, VA (United States)

    2010-07-01

    The solid-solid separation processes employed by modern coal preparation plants require large amounts of process water. After cleaning, the unwanted water must be removed from the surfaces of the particles using mechanical dewatering equipment such as filters or centrifuges. Unfortunately, the existing processes that are used to dewater fine particles are inefficient in terms of moisture reduction and/or solids recovery. In light of these problems, a new ultrafine dewatering process called the Centribaric technology has been developed. This novel device combines centrifugation and pressure filtration within one process to substantially reduce moisture over what can be achieved using conventional dewatering systems. This article discusses the evolution of the Centribaric technology from early research through commercial development and presents data from recent laboratory, pilot-plant, and in-plant test programs.

  10. Technology Development for a Neutrino Astrophysical Observatory

    International Nuclear Information System (INIS)

    Chaloupka, V.; Cole, T.; Crawford, H.J.; He, Y.D.; Jackson, S.; Kleinfelder, S.; Lai, K.W.; Learned, J.; Ling, J.; Liu, D.; Lowder, D.; Moorhead, M.; Morookian, J.M.; Nygren, D.R.; Price, P.B.; Richards, A.; Shapiro, G.; Shen, B.; Smoot, George F.; Stokstad, R.G.; VanDalen, G.; Wilkes, J.; Wright, F.; Young, K.

    1996-01-01

    We propose a set of technology developments relevant to the design of an optimized Cerenkov detector for the study of neutrino interactions of astrophysical interest. Emphasis is placed on signal processing innovations that enhance significantly the quality of primary data. These technical advances, combined with field experience from a follow-on test deployment, are intended to provide a basis for the engineering design for a kilometer-scale Neutrino Astrophysical Observatory

  11. High technology in developing countries: Analysis of technology strategy, technology transfer, and success factors in the aircraft industry

    NARCIS (Netherlands)

    Steenhuis, H.J.; de Bruijn, E.J.

    2004-01-01

    Economical development is highly related to technological development. It is therefore not surprising that many of the industrially developing nations follow explicit strategies to increase their technological competence level. Industrially developing countries may even pursue a strategy of

  12. The development of nuclear technology transfer

    International Nuclear Information System (INIS)

    Nack-chung Sung

    1987-01-01

    Korea, as a recipient of nuclear technology transfer, has good experience of progressively building up its indigeneous capability of nuclear technology through three stages of technology transfer, namely: technology transfer under the turnkey approach, component approach, and integrated technology transfer with a local prime contractor. Here, each stage of experience of technology transfer, with Korea as a recipient, is presented. (author)

  13. Medically relevant ElectroNeedle technology development.

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Carrie Frances; Thomas, Michael Loren; McClain, Jaime L.; Harper, Jason C.; Achyuthan, Komandoor E.; Ten Eyck, Gregory A.

    2008-11-01

    ElectroNeedles technology was developed as part of an earlier Grand Challenge effort on Bio-Micro Fuel Cell project. During this earlier work, the fabrication of the ElectroNeedles was accomplished along with proof-of-concept work on several electrochemically active analytes such as glucose, quinone and ferricyanide. Additionally, earlier work demonstrated technology potential in the field of immunosensors by specifically detecting Troponin, a cardiac biomarker. The current work focused upon fabrication process reproducibility of the ElectroNeedles and then using the devices to sensitively detect p-cresol, a biomarker for kidney failure or nephrotoxicity. Valuable lessons were learned regarding fabrication assurance and quality. The detection of p-cresol was accomplished by electrochemistry as well as using fluorescence to benchmark ElectroNeedles performance. Results from these studies will serve as a guide for the future fabrication processes involving ElectroNeedles as well as provide the groundwork necessary to expand technology applications. One paper has been accepted for publication acknowledging LDRD funding (K. E. Achyuthan et al, Comb. Chem. & HTS, 2008). We are exploring the scope for a second paper describing the applications potential of this technology.

  14. Development of Reconstitution Technology for Surveillance Specimens

    International Nuclear Information System (INIS)

    Yasushi Atago; Shunichi Hatano; Eiichiro Otsuka

    2002-01-01

    The Japan Power Engineering and Inspection Corporation (JAPEIC) has been carrying out the project titled 'Nuclear Power Plant Integrated Management Technology (PLIM)' consigned by Japanese Ministry of Economy, Trade and Industry (METI) since 1996FY as a 10-years project. As one of the project themes, development of reconstitution technology for reactor pressure vessel (RPV/RV) surveillance specimens, which are installed in RPVs to monitor the neutron irradiation embrittlement on RPV/RV materials, is now on being carried out to deal with the long-term operation of nuclear power plants. The target of this theme is to establish the technical standard for applicability of reconstituted surveillance specimens including the reconstitution of the Charpy specimens and Compact Tension (CT) specimens. With the Charpy specimen reconstitution, application of 10 mm length inserts is used, which enables the conversion of tests from the LT-direction to the TL-direction. This paper presents the basic data from Charpy and CT specimens of RPV materials using the surveillance specimens obtained for un-irradiated materials including the following. 1) Reconstitution Technology of Charpy Specimens. a) The interaction between plastic zone and Heat Affected Zone (HAZ). b) The effects of the possible deviations from the standard specimens for the reconstituted specimens. 2) Reconstitution Technology of CT specimens. a) The correlation between fracture toughness and plastic zone width. Because the project is now in progress, this paper describes the outline of the results obtained as of the end of 2000 FY. (authors)

  15. Developing Technology Products - A Physicist's Perspective

    Science.gov (United States)

    Burka, Michael

    2014-03-01

    There are many physicists working in the industrial sector. We rarely have the word physicist in our job title; we are far more commonly called engineers or scientists. But, we are physicists, and we succeed because our training in physics has given us the habits of mind and the technical skills that one needs to solve complex technical challenges. This talk will explore the transition from physics research to technology product development using examples from my own career, first as a postdoctoral fellow and research scientist on the LIGO project, and then developing products in the spectroscopy, telecommunications, and medical device industries. Approaches to identifying and pursuing opportunities in industry will be discussed.

  16. Game development and simulation with unreal technology

    CERN Document Server

    Tavakkoli, Alireza

    2015-01-01

    Game Development and Simulation with Unreal Technology explores the use of Unreal Engine 4 (UE4) for the development of real-time digital interactive contents to be used in computerized games or simulations. The engine is considered in three main iterations: from the basic use of the engine to build games and simulation content out of the box, to intermediate design of interactive content by building on the pre-supplied contents from the base engine, and through advanced implementations geared toward a comprehensive understanding of the power of the Unreal Engine. This is one of the first book

  17. Developing Digital Technologies for Undergraduate University Mathematics

    DEFF Research Database (Denmark)

    Triantafyllou, Eva; Timcenko, Olga

    2013-01-01

    adopt a participatory design method, involving collaboration with students and teachers. As a first step in our design, we developed in collaboration with teachers a set of visualization applets using GeoGebra for the “Mathematics for Multimedia Applications” course taught for Media Technology students...... during lectures and exercise time. During these observations we were able to investigate how the applets were used in practice but also to get insight in the challenges that the students face during mathematics learning. These findings together with student feedback inspire the next round of design...... requirements for the development of digital tools that support mathematics teaching and learning at university level....

  18. Development of decontamination, decommissioning and environmental restoration technology

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Byung Jik; Kwon, H. S.; Kim, G. N. and others

    1999-03-01

    Through the project of 'Development of decontamination, decommissioning and environmental restoration technology', the followings were studied. 1. Development of decontamination and repair technology for nuclear fuel cycle facilities 2. Development of dismantling technology 3. Development of environmental restoration technology. (author)

  19. Development of the advanced CANDU technology -Development of basic technology for HWR design

    International Nuclear Information System (INIS)

    Seok, Ho Cheon; Seok, Soo Dong; Lee, Sang Yong

    1996-07-01

    It is believed that it is easier for Korea to become self-reliant in PHWR technology than in PWR technology, mainly because of the lower design pressure and temperature and because of the simplicity, economy, flexibility of the fuel cycle in comparison with PWR systems. Even though one has no doubt about the safety and the economics of the PHWR's that are now being operated or constructed in Korea. It is necessary to develop the advanced design technology for even safer and more economical PHWR systems to overcome the ever growing international resistance to sharing of nuclear technology and to meet the even more stringent requirements for the future public acceptance of nuclear power. This study is to develop the more advance design technology compared to the existing one, especially in the field of reactor physics, safety systems and safety evaluation to realize the above requirements. 71 tabs., 147 figs., 143 refs. (Author)

  20. Development of the advanced CANDU technology -Development of basic technology for HWR design-

    Energy Technology Data Exchange (ETDEWEB)

    Suk, Hoh Chun; Lee, Sang Yong; Suk, Soo Dong [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-07-01

    It is believed that it is easier for Korea to become self-reliant in PHWR technology than in PWR technology, mainly because of the lower design pressure and temperature and because of the simplicity, economy, flexibility of the fuel cycle in comparison with PWR systems. Even though one has no doubt about the safety and the economics of the PHWR`s that are now being operated or constructed in Korea, it is necessary to develop the advanced design technology for even safer and more economical PHWR systems to overcome the ever growing international resistance to sharing of nuclear technology and to meet the even more stringent requirements for the future public acceptance of nuclear power. This study is to develop the more advance design technology compared to the existing one, by performing in-depth studies especially in the field of reactor physics, safety systems and safety evaluation to realize the above requirements. 90 figs, 50 tabs, 38 refs. (Author).

  1. Development of multiplex serological assay for the detection of human African trypanosomiasis.

    Science.gov (United States)

    Nzou, Samson Muuo; Fujii, Yoshito; Miura, Masashi; Mwau, Matilu; Mwangi, Anne Wanjiru; Itoh, Makoto; Salam, Md Abdus; Hamano, Shinjiro; Hirayama, Kenji; Kaneko, Satoshi

    2016-04-01

    Human African trypanosomiasis (HAT) is a disease caused by Kinetoplastid infection. Serological tests are useful for epidemiological surveillance. The aim of this study was to develop a multiplex serological assay for HAT to assess the diagnostic value of selected HAT antigens for sero-epidemiological surveillance. We cloned loci encoding eight antigens from Trypanosoma brucei gambiense, expressed the genes in bacterial systems, and purified the resulting proteins. Antigens were subjected to Luminex multiplex assays using sera from HAT and VL patients to assess the antigens' immunodiagnostic potential. Among T. b. gambiense antigens, the 64-kDa and 65-kDa invariant surface glycoproteins (ISGs) and flagellar calcium binding protein (FCaBP) had high sensitivity for sera from T. b. gambiense patients, yielding AUC values of 0.871, 0.737 and 0.858 respectively in receiver operating characteristics (ROC) analysis. The ISG64, ISG65, and FCaBP antigens were partially cross-reactive to sera from Trypanosoma brucei rhodesiense patients. The GM6 antigen was cross-reactive to sera from T. b. rhodesiense patients as well as to sera from VL patients. Furthermore, heterogeneous antibody responses to each individual HAT antigen were observed. Testing for multiple HAT antigens in the same panel allowed specific and sensitive detection. Our results demonstrate the utility of applying multiplex assays for development and evaluation of HAT antigens for use in sero-epidemiological surveillance. Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  2. Development of a sensitive molecular detection assay for mango malformation disease caused by Fusarium mangiferae.

    Science.gov (United States)

    Wu, Jingbo; Liu, Feng; Zhan, Rulin; Li, Guoping; Zhao, Yanlong; Jinmei, Chang; He, Yanbiao

    2016-05-01

    To develop a sensitive and specific molecular assay for detection of mango malformation disease (MMD), which is caused primarily by Fusarium mangiferae. We screened 100 ISSR primers and identified one (UBC888) that directed the stable amplification of a specific gene fragment of 479 bp (GenBank accession number KJ526382). Based on the DNA sequence of this fragment, a pair of SCAR primers (W342 and W1772) were designed to amplify another gene fragment of 1376 bp (GenBank accession number KJ526383), demonstrating the successful conversion of an ISSR marker to a SCAR marker. An effective and simple detection assay for MMD was established based on this pair of PCR primers, with a high level of specificity and sensitivity to the DNA of F. mangiferae and other species of Fusarium both in vitro and in vivo. It can detect as little as 10 pg fungal DNA from the DNA of mango's tissues. Our assay provides a practical method for the early diagnosis so that proper prevention of the mango malformation disease can be developed.

  3. Web Development Technology-PHP. How It Is Related To Web Development Technology ASP.NET

    Directory of Open Access Journals (Sweden)

    Manya Sharma

    2015-01-01

    Full Text Available ABSTRACT This paper tells about the technologies used in PHP and how they are related to ASP.NET. The paper begin with the introduction of PHP defining what and how technologies has been used in development of User Complaint Web Application. How thistechnology is related to ASP.NET in features such as implementation functionality validation and proactive behavior involved in validating user input from the browser providing users feedback overall time consumed in development and maintenance.

  4. On the possibility of using polycrystalline material in the development of structure-based generic assays

    International Nuclear Information System (INIS)

    Allaire, Marc; Moiseeva, Natalia; Botez, Cristian E.; Engel, Matthew A.; Stephens, Peter W.

    2009-01-01

    The correlation coefficients calculated between raw powder diffraction profiles can be used to identify ligand-bound/unbound states of lysozyme. The discovery of ligands that bind specifically to a targeted protein benefits from the development of generic assays for high-throughput screening of a library of chemicals. Protein powder diffraction (PPD) has been proposed as a potential method for use as a structure-based assay for high-throughput screening applications. Building on this effort, powder samples of bound/unbound states of soluble hen-egg white lysozyme precipitated with sodium chloride were compared. The correlation coefficients calculated between the raw diffraction profiles were consistent with the known binding properties of the ligands and suggested that the PPD approach can be used even prior to a full description using stereochemically restrained Rietveld refinement

  5. Development of a prototype hybrid L-edge/L-XRF densitometer for nuclear fuel assay.

    Science.gov (United States)

    Joung, Sungyeop; Park, Seunghoon

    2018-03-01

    The hybrid L-edge/L-XRF densitometer (HLED) was developed for on-site nuclear fuel assays intended for safeguards purpose. The HLED can simultaneously measure both X-ray photon transmissions and characteristic X-ray emissions, which characterizes the elemental composition of samples of interest to determine the concentration of actinide-bearing materials, such as plutonium and uranium, in a nuclear fuel. A prototype of the HLED equipment was fabricated and tested to study the feasibility of nuclear material assays using a surrogate material (lead) to avoid radiation effects from nuclear materials. The uncertainty of the L-edge and L-XRF characteristics of the sample material are discussed in the article. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay.

    Science.gov (United States)

    Tengs, T; Bowers, H A; Ziman, A P; Stoecker, D K; Oldach, D W

    2001-02-01

    Nuclear and chloroplast-encoded small subunit ribosomal DNA sequences were obtained from several strains of the toxic dinoflagellate Gymnodinium galatheanum. Phylogenetic analyses and comparison of sequences indicate that the chloroplast sequences show a higher degree of sequence divergence than the nuclear homologue. The chloroplast sequences were chosen as targets for the development of a 5'--3' exonuclease assay for detection of the organism. The assay has a very high degree of specificity and has been used to screen environmental water samples from a fish farm where the presence of this dinoflagellate species has previously been associated with fish kills. Various hypotheses for the derived nature of the chloroplast sequences are discussed, as well as what is known about the toxicity of the species.

  7. Fission Surface Power Technology Development Update

    Science.gov (United States)

    Palac, Donald T.; Mason, Lee S.; Houts, Michael G.; Harlow, Scott

    2011-01-01

    Power is a critical consideration in planning exploration of the surfaces of the Moon, Mars, and places beyond. Nuclear power is an important option, especially for locations in the solar system where sunlight is limited or environmental conditions are challenging (e.g., extreme cold, dust storms). NASA and the Department of Energy are maintaining the option for fission surface power for the Moon and Mars by developing and demonstrating technology for a fission surface power system. The Fission Surface Power Systems project has focused on subscale component and subsystem demonstrations to address the feasibility of a low-risk, low-cost approach to space nuclear power for surface missions. Laboratory demonstrations of the liquid metal pump, reactor control drum drive, power conversion, heat rejection, and power management and distribution technologies have validated that the fundamental characteristics and performance of these components and subsystems are consistent with a Fission Surface Power preliminary reference concept. In addition, subscale versions of a non-nuclear reactor simulator, using electric resistance heating in place of the reactor fuel, have been built and operated with liquid metal sodium-potassium and helium/xenon gas heat transfer loops, demonstrating the viability of establishing system-level performance and characteristics of fission surface power technologies without requiring a nuclear reactor. While some component and subsystem testing will continue through 2011 and beyond, the results to date provide sufficient confidence to proceed with system level technology readiness demonstration. To demonstrate the system level readiness of fission surface power in an operationally relevant environment (the primary goal of the Fission Surface Power Systems project), a full scale, 1/4 power Technology Demonstration Unit (TDU) is under development. The TDU will consist of a non-nuclear reactor simulator, a sodium-potassium heat transfer loop, a power

  8. Tracing Impacts of Science and Technology Development

    Science.gov (United States)

    Powell, Jeanne

    2003-03-01

    ATP's Mission and Operations. The ATP partners with industry to accelerate the development of innovative technologies for broad national economic benefit. The program's focus is on co-funding collaborative, multi-disciplinary technologies and enabling technology platforms that appear likely to be commercialized, with private sector funding, once the high technical risks are reduced. Industry-led projects are selected for funding in rigorous competitions on the basis of technical and economic merit. Since 1990, ATP has co-funded 642 projects, with 1,329 participants and another 1,300 subcontractors. Measuring to Mission: Overview of ATP's Evaluation Program. ATP's multi-component evaluation strategy provides measures of progress and performance matched to the stage of project evolution; i.e., for the short-term, from the time of project selection and over the course of the R for the mid-term, as commercial applications are pursued, early products reach the market, and dissemination of knowledge created in the R projects occurs; and for the longer-term, as more fully-developed technologies diffuse across multiple products and industries. The approach is applicable to all public S programs and adaptable to private or university projects ranging from basic research to applied industrial R. Examples of Results. ATP's composite performance rating system assesses ATP's completed projects against multi-faceted performance criteria of Knowledge Creation and Dissemination and Commercialization Progress 2-3 years after the end of ATP-funded R. It generates scores ranging from zero to four stars. Results for ATP's first 50 completed projects show that 16are in the bottom group of zero or one stars. 60the middle group. It is understood that not all ATP projects will be successful given the program's emphasis on funding high-risk technology development that the private sector is unwilling and unable to fund alone. Different technologies have different timelines for

  9. Technology road mapping to guide development planning

    International Nuclear Information System (INIS)

    Goossen, J.E.; Congedo, T.V.

    2004-01-01

    For the past five years, Westinghouse Electric Company, has made ever increasing use of Technology Road Mapping, to direct company development efforts to achieve maximum benefits for our customers and ourselves. Comprised of business units in Nuclear Fuels, Nuclear Services and Nuclear Power Plants, including domestic and international business segments, Westinghouse must pay particular attention to coordinating development to satisfy the diverse needs of our growing international customer base. We must develop products which both benefit the individual Business Unit customer base, and which create synergy to produce the best possible offerings to the broader marketplace. The knowledge we gain through customer contacts and direct customer participation provides the basis from which we develop the Technology Road Map. This Road Map development process can be compared to painting a picture, where the background colors and features correspond to drivers related to the Customer and the prevailing features of the market environment. The subsequent layers of detail include broad Technical Objectives and then specific Technical Goals which will support achieving those objectives. The process is described in detail, and examples are provided. (authors)

  10. Decommissioning technology development for research reactors

    International Nuclear Information System (INIS)

    Lee, K. W.; Kim, S. K.; Kim, Y. K.

    2004-03-01

    Although it is expected that the decommissioning of a nuclear power plant will happen since 2020, the need of partial decommissioning and decontamination for periodic inspection and life extension has been on an increasing trend and domestic market has gradually been extended. Therefore, in this project the decommissioning DB system on the KRR-1 and 2 was developed as establishing the information classification system of the research reactor dismantling and the structural design and optimization of the decommissioning DB system. Also in order to secure the reliability and safety about the dismantling process, the main dismantling simulation technology that can verify the dismantling process before their real dismantling work was developed. And also the underwater cutting equipment was developed to remove these stainless steel parts highly activated from the RSR. First, the its key technologies were developed and then the design, making, and capability analysis were performed. Finally the actual proof was achieved for applying the dismantling site. an automatic surface contamination measuring equipment was developed in order to get the sample automatically and measure the radiation/radioactivity

  11. Development and characterization of a high density SNP genotyping assay for cattle.

    Directory of Open Access Journals (Sweden)

    Lakshmi K Matukumalli

    Full Text Available The success of genome-wide association (GWA studies for the detection of sequence variation affecting complex traits in human has spurred interest in the use of large-scale high-density single nucleotide polymorphism (SNP genotyping for the identification of quantitative trait loci (QTL and for marker-assisted selection in model and agricultural species. A cost-effective and efficient approach for the development of a custom genotyping assay interrogating 54,001 SNP loci to support GWA applications in cattle is described. A novel algorithm for achieving a compressed inter-marker interval distribution proved remarkably successful, with median interval of 37 kb and maximum predicted gap of <350 kb. The assay was tested on a panel of 576 animals from 21 cattle breeds and six outgroup species and revealed that from 39,765 to 46,492 SNP are polymorphic within individual breeds (average minor allele frequency (MAF ranging from 0.24 to 0.27. The assay also identified 79 putative copy number variants in cattle. Utility for GWA was demonstrated by localizing known variation for coat color and the presence/absence of horns to their correct genomic locations. The combination of SNP selection and the novel spacing algorithm allows an efficient approach for the development of high-density genotyping platforms in species having full or even moderate quality draft sequence. Aspects of the approach can be exploited in species which lack an available genome sequence. The BovineSNP50 assay described here is commercially available from Illumina and provides a robust platform for mapping disease genes and QTL in cattle.

  12. Quantifying MMA by SLE LC-MS/MS: Unexpected challenges in assay development.

    Science.gov (United States)

    Lo, Sheng-Ying; Gordon, Cindy; Sadilkova, Katerina; Jack, Rhona M; Dickerson, Jane A

    2016-09-01

    Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simple method preparation. MMA and stable isotope labeled d3-MMA was extracted using supported liquid extraction (SLE). Assay imprecision, bias, linearity, recovery and carryover were determined. The relationship between MMA and propionyl acylcarnitine (C3-acylcarnitine) was also evaluated using historical paired results from 51 unique individuals. Baseline separation between MMA and succinic acid was completed in 7min. The assay was linear from 0.1 to 500μM. The intra-day and inter-day imprecision CV ranged from 4.1 to 13.2% (0.3 to 526μM) and 5.0 to 15.7% (0.3 to 233μM), respectively. Recovery ranged from 93 to 125%. The correlation with a national reference laboratory LC-MS/MS assay showed a Deming regression of 1.026 and intercept of -1.335. Carryover was determined to be MMA and C3-acylcarnitine. This report describes the first LC-MS/MS method using SLE for MMA extraction. In addition, we illustrate the challenges encountered during this method development that should be assessed and resolved by any laboratory implementing a SLE LC-MS/MS assay designed to quantify analytes across several orders of magnitude. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  13. Development of an ultrasensitive PCR assay for polycyclic musk determination in fish.

    Science.gov (United States)

    Zhang, Xiaohan; Zhuang, Huisheng

    2018-02-21

    Polycyclic musks (PCMs) in the aquatic environment and organisms have become an emerging environmental issue because of their potential risk. The most used method for polycyclic musk determination is gas chromatography-mass spectrometry (GC-MS) with different sample extractions, which are somewhat expensive to operate, complex and laborious. In this study, a novel and ultrasensitive real-time polymerase chain reaction (PCR) assay with multiple signal amplification of carboxylic-DNA by gold nanoparticle-polyamidoamine conjugation (Au-PAMAM) was developed for determining polycyclic musks in fish. Hapten and immunogen were specially prepared. Polyclonal antibodies were produced based on the optimal immunisation, and the antibodies were characterised. Due to PAMAM's unique nanostructure of numerous functional amino groups, polyclonal antibody and carboxylic-DNA were immobilised by Au-PAMAM conjugation to develop the antibody-Au-PAMAM-DNA probes, which were used as a signal DNA amplifier in the PCR system. Compared with real-time immuno-PCR, this biological probe-amplified immuno-PCR (BPAI-PCR) assay had higher sensitivity due to the probes' higher ratio of signal DNA. Finally, the BPAI-PCR assay was applied to analyse AHTN (7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene,Tonalide) concentrations in fish samples in the range from 1 pg/L to 10 ng/L, giving an of LOD 0.61 pg/L. In general, due to the specificity of the antibody and novel nanoprobe design, this BPAI-PCR assay provided a potential way for trace analysis of AHTN in the aquatic organisms. The high concentrations of AHTN found in cultivated fish should encourage further toxicological studies.

  14. Development of at-line assay to monitor charge variants of MAbs during production.

    Science.gov (United States)

    St Amand, M M; Ogunnaike, B A; Robinson, A S

    2014-01-01

    One major challenge currently facing the biopharmaceutical industry is to understand how MAb microheterogeneity affects therapeutic efficacy, potency, immunogenicity, and clearance. MAb micro-heterogeneity can result from post-translational modifications such as sialylation, galactosylation, C-terminal lysine cleavage, glycine amidation, and tryptophan oxidation, each of which can generate MAb charge variants; such heterogeneity can affect pharmacokinetics (PK) considerably. Implementation of appropriate on-line quality control strategies may help to regulate bioprocesses, thus enabling more homogenous material with desired post-translational modifications and PK behavior. However, one major restriction to implementation of quality control strategies is the availability of techniques for obtaining on-line or at-line measurements of these attributes. In this work, we describe the development of an at-line assay to separate MAb charge variants in near real-time, which could ultimately be used to implement on-line quality control strategies for MAb production. The assay consists of a 2D-HPLC method with sequential in-line Protein A and WCX-10 HPLC column steps. To perform the 2D-HPLC assay at-line, the two columns steps were integrated into a single method using a novel system configuration that allowed parallel flow over column 1 or column 2 or sequential flow from column 1 to column 2. A bioreactor system was also developed such that media samples could be removed automatically from bioreactor vessels during production and delivered to the 2D-HPLC for analysis. With this at-line HPLC assay, we have demonstrated that MAb microheterogeneity occurs throughout the cell cycle whether the host cell line is grown under different or the same nominal culture conditions. © 2013 American Institute of Chemical Engineers.

  15. Development of radiological emergency preparedness technology

    International Nuclear Information System (INIS)

    Han, Moon Hee; Kim, In Gyoo; Kim, Kook Chan; Kim, Eun Han; Suh, Kyung Suk; Hwang, Won Tae; Choi, Young gil; Shim, Hae Won; Lee, Jeong Ho; Lee, Kang Suk

    2000-04-01

    Large-scale field tracer experiments have been conducted on Ulchin, Wolsung and Daeduk sites for the purpose of validating FADAS and of analyzing the environmental characteristics around the nuclear sites. The most influential factor in atmospheric dispersion is the meteorological condition. During the experiment, meteorological data were measured on the release point and the selected positions among sampling points. Once radioactive materials are released to the atmosphere, members of public may be exposed through the environmental media such as air, soil and foods. Therefore, to protect the public, adequate countermeasures should be taken at due time for those exposure pathways. both processes, of justification and optimization are applied to a countermeasure simultaneously for decision-making. The work scope of Biological research for the radiation protection had contained the search of biological microanalytic methods for assessing the health effect by γ-radiation and toxic agents, the standardization of human T-lymphocyte cell culture and polymerase chain reaction, T-cell clonal assay, and the quantification of mutation frequency in the hypoxanthine (guanine) phosphoribosyl transferase (HPRT) gene locus by single exposure or combined exposure. Especially, the polymerase chain reaction methods using reverse transcriptase has been developed to analyze the mutant gene induced by γ-radiation and pentachlorophenol(PCP) exposure, and to investigate the point mutations in the HPRT gene locus of T-lymphocytes. The HPRT T-cell clonal assay revealed that could not differentiate γ-irradiation from PCP, because the frequency of somatic mutations induced by both damaging agents increased in a dose-dependent manner. RT/PCR technique will be needed for the mutation spectrum of HPRT gene essential to discriminate that by radiation exposure from health effect by chemical exposure on man. (author)

  16. Development of radiological emergency preparedness technology

    Energy Technology Data Exchange (ETDEWEB)

    Han, Moon Hee; Kim, In Gyoo; Kim, Kook Chan; Kim, Eun Han; Suh, Kyung Suk; Hwang, Won Tae; Choi, Young gil; Shim, Hae Won; Lee, Jeong Ho; Lee, Kang Suk

    2000-04-01

    Large-scale field tracer experiments have been conducted on Ulchin, Wolsung and Daeduk sites for the purpose of validating FADAS and of analyzing the environmental characteristics around the nuclear sites. The most influential factor in atmospheric dispersion is the meteorological condition. During the experiment, meteorological data were measured on the release point and the selected positions among sampling points. Once radioactive materials are released to the atmosphere, members of public may be exposed through the environmental media such as air, soil and foods. Therefore, to protect the public, adequate countermeasures should be taken at due time for those exposure pathways. both processes, of justification and optimization are applied to a countermeasure simultaneously for decision-making. The work scope of Biological research for the radiation protection had contained the search of biological microanalytic methods for assessing the health effect by {gamma}-radiation and toxic agents, the standardization of human T-lymphocyte cell culture and polymerase chain reaction, T-cell clonal assay, and the quantification of mutation frequency in the hypoxanthine (guanine) phosphoribosyl transferase (HPRT) gene locus by single exposure or combined exposure. Especially, the polymerase chain reaction methods using reverse transcriptase has been developed to analyze the mutant gene induced by {gamma}-radiation and pentachlorophenol(PCP) exposure, and to investigate the point mutations in the HPRT gene locus of T-lymphocytes. The HPRT T-cell clonal assay revealed that could not differentiate {gamma}-irradiation from PCP, because the frequency of somatic mutations induced by both damaging agents increased in a dose-dependent manner. RT/PCR technique will be needed for the mutation spectrum of HPRT gene essential to discriminate that by radiation exposure from health effect by chemical exposure on man. (author)

  17. Small Hydropower Research and Development Technology Project

    Energy Technology Data Exchange (ETDEWEB)

    Blackmore, Mo [Near Space Systems, Inc.

    2013-12-06

    The objective of this work was to investigate, develop, and validate the next generation of small hydroturbine generator designs that maximize the energy transfer from flowing water to electrical power generation. What resulted from this effort was the design of a new technology hydroturbine that Near Space Systems (NSS) has named the Star*Stream© Hydroturbine. Using a design that eliminates nearly all of the shortfalls of conventional hydroturbines, the Star*Stream© Hydroturbine employs a new mechanical-to-electrical energy transfer hydro design that operates without lubrication of any kind, and does not introduce foreign chemicals or particulate matter from oil or drive shaft seal degradation into the hydro ecology. In its unique configuration, the Star*Stream© Hydroturbine is nearly environmentally inert, without the negative aspects caused by interrupting the ecological continuity, i.e., disruptions to sedimentation, water quality, habitat changes, human displacement, fish migration, etc., - while it ensures dramatically reduced timeframes to project completion. While a remarkable reduction in LCOE resulting from application of the Star*Stream© Hydroturbine technology has been the core achievement of the this effort, there have been numerous technological breakthroughs from the development effort.

  18. Application of nondestructive assay technology in Oak Ridge National Laboratory's waste management program

    International Nuclear Information System (INIS)

    Schultz, F.J.; Smith, M.A.; Brandenburg, R.W.; Caylor, B.A.; Coffey, D.E.; Hensley, D.C.; Phoenix, L.B.

    1990-01-01

    Waste characterization is the process whereby physical properties and chemical composition of waste are determined. Waste characterization is an important element of a waste certification program in that it provides information which is necessary to certify that waste meets the acceptance criteria for storage, treatment, or disposal. Department of Energy (DOE) Order 5820.2A and WIPP-DOE-069 list and describe the germane waste form, package, and container criteria for the storage of both solid low-level waste (SLLW) and transuranic (TRU) waste, including chemical composition and compatibility, hazardous material content, fissile material content, equivalent alpha activity, thermal heat output, and absence of free liquids, explosives, and compressed gases. At the Oak Ridge National Laboratory (ORNL), the responsibility for waste characterization begins with the individual(s) who generate the waste. The generator must be able to document the type and estimate the quantity of various materials which have been placed into the waste container. Analyses of process flow sheets and a statistically valid sampling program can provide much of the required information as well as a documented level of confidence in the acquired data. A program is being instituted in which the major generator facilities perform radionuclide assay of small packets of waste prior to being placed into a waste drum. 10 refs., 1 fig., 1 tab

  19. Development of public health assurance technology by radiation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Ho; Lim, Sang Yong; Yang, Jae Seung (and others)

    2007-07-15

    This project was performed to develop the radiation sterilization process of public health products and RT/BT fusion technology and to secure a detection and quarantine system of irradiated food. To establish the radiation sterilization of public health goods, current status of radiation sterilization of disposable medical equipment was investigated and the manufacturing process of disposable media for microbial cultivation were developed using a gamma sterilization. In addition, microbial contamination of disposable kitchen utensils was surveyed and pathogen-free organic compost was developed by radiation sterilization. The radiation responses of bacteria including Salmonella, Vibrio, E. coli, and D. radiodurans were analyzed by DNA chip and 2-D electrophoresis. To validate the safety of surviving bacteria after irradiation, the expressions of virulence genes of pathogenic bacteria were monitored using real-time PCR, and the growth of mycotoxin-producing funguses was studied after irradiation. And also, quantitative detection methods of irradiated and inactivated Salmonella using a real-time PCR and a immuno assay. To establish the quarantine and quality assurance of irradiated food and public health products, radiation technology was applied to the fermented foods, minimally processed food and dried vegetables. Radiation effects on insects was examined and the corresponding data base was constructed. We also collaborated on the preliminary test of international trade of sea food with USA or India. To establish the official detection method of irradiated food, physical, chemical and biological detection methods for irradiated food were verified. Finally, multiple range test of irradiated food was performed.

  20. Development of public health assurance technology by radiation

    International Nuclear Information System (INIS)

    Kim, Dong Ho; Lim, Sang Yong; Yang, Jae Seung

    2007-07-01

    This project was performed to develop the radiation sterilization process of public health products and RT/BT fusion technology and to secure a detection and quarantine system of irradiated food. To establish the radiation sterilization of public health goods, current status of radiation sterilization of disposable medical equipment was investigated and the manufacturing process of disposable media for microbial cultivation were developed using a gamma sterilization. In addition, microbial contamination of disposable kitchen utensils was surveyed and pathogen-free organic compost was developed by radiation sterilization. The radiation responses of bacteria including Salmonella, Vibrio, E. coli, and D. radiodurans were analyzed by DNA chip and 2-D electrophoresis. To validate the safety of surviving bacteria after irradiation, the expressions of virulence genes of pathogenic bacteria were monitored using real-time PCR, and the growth of mycotoxin-producing funguses was studied after irradiation. And also, quantitative detection methods of irradiated and inactivated Salmonella using a real-time PCR and a immuno assay. To establish the quarantine and quality assurance of irradiated food and public health products, radiation technology was applied to the fermented foods, minimally processed food and dried vegetables. Radiation effects on insects was examined and the corresponding data base was constructed. We also collaborated on the preliminary test of international trade of sea food with USA or India. To establish the official detection method of irradiated food, physical, chemical and biological detection methods for irradiated food were verified. Finally, multiple range test of irradiated food was performed

  1. Fission Surface Power Technology Development Status

    Science.gov (United States)

    Palac, Donald T.; Mason, Lee S.; Harlow, Scott

    2009-01-01

    With the potential future deployment of a lunar outpost there is expected to be a clear need for a high-power, lunar surface power source to support lunar surface operations independent of the day-night cycle, and Fission Surface Power (FSP) is a very effective solution for power levels above a couple 10 s of kWe. FSP is similarly enabling for the poorly illuminated surface of Mars. The power levels/requirements for a lunar outpost option are currently being studied, but it is known that cost is clearly a predominant concern to decision makers. This paper describes the plans of NASA and the DOE to execute an affordable fission surface power system technology development project to demonstrate sufficient technology readiness of an affordable FSP system so viable and cost-effective FSP system options will be available when high power lunar surface system choices are expected to be made in the early 2010s.

  2. Technology development activities supporting tank waste remediation

    International Nuclear Information System (INIS)

    Bonner, W.F.; Beeman, G.H.

    1994-06-01

    This document summarizes work being conducted under the U.S. Department of Energy's Office of Technology Development (EM-50) in support of the Tank Waste Remediation System (TWRS) Program. The specific work activities are organized by the following categories: safety, characterization, retrieval, barriers, pretreatment, low-level waste, and high-level waste. In most cases, the activities presented here were identified as supporting tank remediation by EM-50 integrated program or integrated demonstration lead staff and the selections were further refined by contractor staff. Data sheets were prepared from DOE-HQ guidance to the field issued in September 1993. Activities were included if a significant portion of the work described provides technology potentially needed by TWRS; consequently, not all parts of each description necessarily support tank remediation

  3. Photonics technology development for optical fuzing.

    Energy Technology Data Exchange (ETDEWEB)

    Liu, J.J. (U.S. Army Research Laboratory, Adelphi, MD); Geib, Kent Martin; von der Lippe, C.M. (U.S. Army ARDEC, Adelphi, MD); Peake, Gregory Merwin; Serkland, Darwin Keith; Keeler, Gordon Arthur; Mar, Alan

    2005-07-01

    This paper describes the photonic component development, which exploits pioneering work and unique expertise at Sandia National Laboratories, ARDEC and the Army Research Laboratory by combining key optoelectronic technologies to design and demonstrate components for this fuzing application. The technologies under investigation for the optical fuze design covered in this paper are vertical cavity surface emitting lasers (VECSELs), integrated resonant cavity photodetectors (RCPD), and diffractive micro-optics. The culmination of this work will be low cost, robust, fully integrated, g-hardened components designed suitable for proximity fuzing applications. The use of advanced photonic components will enable replacement of costly assemblies that employ discrete lasers, photodetectors, and bulk optics. The integrated devices will be mass produced and impart huge savings for a variety of Army applications.

  4. Development of Interconnect Technologies for Particle Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Tripathi, Mani [Univ. of California, Davis, CA (United States)

    2015-01-29

    This final report covers the three years of this grant, for the funding period 9/1/2010 - 8/31/2013. The project consisted of generic detector R&D work at UC Davis, with an emphasis on developing interconnect technologies for applications in HEP. Much of the work is done at our Facility for Interconnect Technologies (FIT) at UC Davis. FIT was established using ARRA funds, with further studies supported by this grant. Besides generic R&D work at UC Davis, FIT is engaged in providing bump bonding help to several DOE supported detector R&D efforts. Some of the developmental work was also supported by funding from other sources: continuing CMS project funds and the Linear Collider R&D funds. The latter program is now terminated. The three year program saw a good deal of progress on several fronts, which are reported here.

  5. Small Orbital Stereo Tracking Camera Technology Development

    Science.gov (United States)

    Gagliano, L.; Bryan, T.; MacLeod, T.

    On-Orbit Small Debris Tracking and Characterization is a technical gap in the current National Space Situational Awareness necessary to safeguard orbital assets and crew. This poses a major risk of MOD damage to ISS and Exploration vehicles. In 2015 this technology was added to NASAs Office of Chief Technologist roadmap. For missions flying in or assembled in or staging from LEO, the physical threat to vehicle and crew is needed in order to properly design the proper level of MOD impact shielding and proper mission design restrictions. Need to verify debris flux and size population versus ground RADAR tracking. Use of ISS for In-Situ Orbital Debris Tracking development provides attitude, power, data and orbital access without a dedicated spacecraft or restricted operations on-board a host vehicle as a secondary payload. Sensor Applicable to in-situ measuring orbital debris in flux and population in other orbits or on other vehicles. Could enhance safety on and around ISS. Some technologies extensible to monitoring of extraterrestrial debris as well To help accomplish this, new technologies must be developed quickly. The Small Orbital Stereo Tracking Camera is one such up and coming technology. It consists of flying a pair of intensified megapixel telephoto cameras to evaluate Orbital Debris (OD) monitoring in proximity of International Space Station. It will demonstrate on-orbit optical tracking (in situ) of various sized objects versus ground RADAR tracking and small OD models. The cameras are based on Flight Proven Advanced Video Guidance Sensor pixel to spot algorithms (Orbital Express) and military targeting cameras. And by using twin cameras we can provide Stereo images for ranging & mission redundancy. When pointed into the orbital velocity vector (RAM), objects approaching or near the stereo camera set can be differentiated from the stars moving upward in background.

  6. Development of kits for radioimmunometric assays for tumour markers. Final report of a co-ordinated research project 1997-2001

    International Nuclear Information System (INIS)

    2002-08-01

    Many tumour marker assays have been reported over the years and their role is well recognized and acknowledged in the follow-up of known cancer cases. However, their true potential for use in primary diagnosis or screening of high risk groups is still to be fully realized due to the need to achieve better specificity. Among the various tumour markers, the one for prostate cancer - prostate specific antigen (PSA) - appears to have better specificity, coming close to a tumour specific antigen. Prostate cancer is a commonly encountered cancer in men, and can be effectively treated if detected early. PSA levels in serum appear to provide good correlation with tumour burden. Estimation of free PSA in serum is reported to further improve the diagnosis. In several developed countries routine screening of men above 50 years of age for prostate cancer using serum PSA as marker is recommended. Radioimmunometric assay techniques offer themselves as attractive candidates for measurement of tumour markers. They are robust, economical and didactic, thus eminently suitable for technology transfer, training and teaching. Preparation of primary reagents is relatively easy. The methodology is flexible. As a result of co-operation projects of the IAEA, many developing Member States have built up indigenous capabilities to perform radioimmunometric assays, which can be extended to development of kits for tumour marker assays. Considering the need for indigenous development of capabilities to produce reliable kits for radioimmunometric assays for PSA, in 1997 the IAEA initiated a Co-ordinated Research Project (CRP) on Development of Kits for Radioimmunometric Assays for Tumour Markers. Even though the focus of the project was PSA, it was expected that the expertise to be gained by the participants would also help them undertake development of kits for other tumour markers, essentially using the same methodology. Ten laboratories from Europe, Asia, Africa and the Americas participated

  7. The development of an expert system for the characterization of waste assay data

    International Nuclear Information System (INIS)

    Bridges, S.; Hodges, J.; Sparrow, C.

    1997-01-01

    Containers of transuranic and low-level alpha contaminated waste generated as a byproduct of Department of Energy defense-related programs must be characterized before their proper disposition can be determined. Nondestructive assay methods are the most desirable means for assessing the mass and activity of the entrained transuranic radionuclides. However, there are other sources of information that may be useful in the characterization of the entrained waste (e.g., container manifests, information about the generation process, and destructive assay techniques performed on representative samples). This paper describes initial work on an expert system being developed to analyze and characterize containerized radiological waste. This system is being developed by scientists at the Mississippi State University Diagnostic and Instrumentation Laboratory (DIAL) in collaboration with scientists at the Idaho National Engineering Laboratory. The DIAL scientists are responsible for (1) the development of techniques to represent and reason with evidence from a variety of sources, and (2) the development of appropriate method(s) to represent and reason with confidence levels associated with that evidence. This paper describes exploratory versions of the expert system developed to evaluate four techniques for representing and reasoning with the confidence in the evidence: MYCIN-style certainty factors, Dempster-Shafer Theory, Bayesian networks, and fuzzy logic. 16 refs., 8 figs., 4 tabs

  8. Multipurpose prevention technologies: products in development.

    Science.gov (United States)

    Friend, David R; Clark, Justin T; Kiser, Patrick F; Clark, Meredith R

    2013-12-01

    Multipurpose prevention technologies (MPTs) are broadly defined as products capable of simultaneously addressing multiple sexual and reproductive health needs including unintended pregnancy, STIs including HIV-1, and other reproductive tract infections. MPTs have been discussed for a few decades but little product development has occurred. With the recent proof-of-concept that a topically applied antiretroviral (ARV) can effectively reduce sexual transmission of HIV-1 (tenofovir 1% gel) the impetus to develop MPTs is gaining momentum. Products currently in development are broadly categorized as either long-acting or on-demand. Long-acting MPTs include intravaginal rings (IVRs) and long-acting injectable products. Several IVR MPTs are under development including one designed to release tenofovir to prevent transmission of HIV-1 and levonorgestrel (LNG) to prevent unintended pregnancy over a 90-day period. Another MPT IVR under development is designed to release the ARV dapivirine and LNG for 2 months. Long-acting injectable pre-exposure prophylaxis (PrEP) formulations of rilpivirine (TMC278) and GSK1265744 have entered clinical evaluation and could form the basis of long-acting injectable products for HIV-1 prevention and prevention of unintended pregnancy. On-demand products include TFV 1% gel (HIV-1/HSV-2 prevention), a zinc/carrageenan zinc gel (HIV-1/HSV-2 prevention), and the SILCS diaphragm administered with TFV 1% gel. Significant technical, funding, and regulatory hurdles must be overcome to develop most MPTs; however, the significant reproductive health benefits to many women around the world should provide motivation to overcome these hurdles. This article is based on a presentation at the "Product Development Workshop 2013: HIV and Multipurpose Prevention Technologies", held in Arlington, Virginia on February 21-22, 2013. It forms part of a special supplement to Antiviral Research. Copyright © 2013. Published by Elsevier B.V.

  9. Development of radioisotope preparation and application technology

    International Nuclear Information System (INIS)

    Han, Hyon Soo; Park, K. B.; Bang, H. S. and others

    2000-04-01

    The purpose of this project is to develop RI production technology utility 'HANARO' and to construct a sound infra-structure for mass production and supply to domestic users. The developed contents and results are as follows: two types of rig for irradiation in reactor core were designed and manufactured. The safety of OR rig during irradiation was identified through various test and it is used for RI production. The prepared IR rig will be used to performance tests for safety. We prepared two welders and welding jigs for production of sealed sources, and equipments for quality control of the welded materials. Production processes and apparatus Cr-51, P-32, I-125 and Sr-89, were developed. Developed results would be used for routine production and supply of radioisotopes. The automatic Tc-99m extraction apparatus was supplied to Libya under IAEA support. For approval on special form radioactive material of the developed Ir-192 source assembly and projector documents were prepared and submitted to MOST. The high dose rate Ir-192 source(diameter 1.1 mm, length 5.2 mm) for RALS and the laser welding system for its fabrication were developed. Production technologies of Ir-192 sources for destructive test and medical therapy were transferred to private company for commercial supply. The chemical immobilization method based on the self-assemble monolayer of ω-functionalized thiol and the sensing scheme based on the beta-emitter labeling method were developed for the fabrication radioimmuno-sensors. Results of this study will be applied to mass production of radioisotopes 'HANARO' and are to contribute the advance of domestic medicine and industry related to radioisotopes

  10. Development of radioisotope preparation and application technology

    Energy Technology Data Exchange (ETDEWEB)

    Han, Hyon Soo; Park, K. B.; Bang, H. S. [and others

    2000-04-01

    The purpose of this project is to develop RI production technology utility 'HANARO' and to construct a sound infra-structure for mass production and supply to domestic users. The developed contents and results are as follows: two types of rig for irradiation in reactor core were designed and manufactured. The safety of OR rig during irradiation was identified through various test and it is used for RI production. The prepared IR rig will be used to performance tests for safety. We prepared two welders and welding jigs for production of sealed sources, and equipments for quality control of the welded materials. Production processes and apparatus Cr-51, P-32, I-125 and Sr-89, were developed. Developed results would be used for routine production and supply of radioisotopes. The automatic Tc-99m extraction apparatus was supplied to Libya under IAEA support. For approval on special form radioactive material of the developed Ir-192 source assembly and projector documents were prepared and submitted to MOST. The high dose rate Ir-192 source(diameter 1.1 mm, length 5.2 mm) for RALS and the laser welding system for its fabrication were developed. Production technologies of Ir-192 sources for destructive test and medical therapy were transferred to private company for commercial supply. The chemical immobilization method based on the self-assemble monolayer of {omega}-functionalized thiol and the sensing scheme based on the beta-emitter labeling method were developed for the fabrication radioimmuno-sensors. Results of this study will be applied to mass production of radioisotopes 'HANARO' and are to contribute the advance of domestic medicine and industry related to radioisotopes.

  11. Development of a recombinant DNA assay system for the detection of genetic change in astronauts' cells

    International Nuclear Information System (INIS)

    Atchley, S.V.; Chen, D.J.C.; Strniste, G.F.; Walters, R.A.; Moyzis, R.K.

    1984-01-01

    We are developing a new recombinant DNA system for the detection and measurement of genetic change in humans caused by exposure to low level ionizing radiation. A unique feature of the method is the use of cloned repetitive DNA probes to assay human DNA for structural changes during or after irradiation. Repetitive sequences exist in different families. Collectively they constitute over 25% of the DNA in a human cell. Repeat families have between 10 and 500,000 members. We have constructed repetitive DNA sequence libraries using recombinant DNA techniques. From these libraries we have isolated and characterized individual repeats comprising 75 to 90% of the mass of human repetitive DNA. Repeats used in our assay system exist in tandem arrays in the genome. Perturbation of these sequences in a cell, followed by detection with a repeat probe, produces a new, multimeric ''ladder'' pattern on an autoradiogram. The repeat probe used in our initial study is complementary to 1% of human DNA. Therefore, the sensitivity of this method is several orders of magnitude better than existing assays. Preliminary evidence from human skin cells exposed to acute, low-dose x-ray treatments indicates that DNA is affected at a dose as low as 5R. The radiation doses used in this system are well within the range of doses received by astronauts during spaceflight missions. Due to its small material requirements, this technique could easily be adapted for use in space. 16 refs., 1 fig

  12. Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples

    Science.gov (United States)

    Pérez, Lester J.; de Arce, Heidy Díaz

    2009-01-01

    Aujeszky’s disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase chain reaction (PCR) assay are presented for the diagnosis of pseudorabies infection. This assay was based on the amplification of a highly conserved viral gD gene fragment. PCR products of the expected size were obtained from PRV strains. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The analytical sensitivity of the test was estimated to be 1.34 TCID50/ 50 uL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. PMID:24031383

  13. Development of an immunochromatographic assay kit using fluorescent silica nanoparticles for rapid diagnosis of Acanthamoeba keratitis.

    Science.gov (United States)

    Toriyama, Koji; Suzuki, Takashi; Inoue, Tomoyuki; Eguchi, Hiroshi; Hoshi, Saichi; Inoue, Yoshitsugu; Aizawa, Hideki; Miyoshi, Kazutomi; Ohkubo, Michio; Hiwatashi, Eiji; Tachibana, Hiroshi; Ohashi, Yuichi

    2015-01-01

    We developed an immunochromatographic assay kit that uses fluorescent silica nanoparticles bound to anti-Acanthamoeba antibodies (fluorescent immunochromatographic assay [FICGA]) and evaluated its efficacy for the detection of Acanthamoeba and diagnosis of Acanthamoeba keratitis (AK). The sensitivity of the FICGA kit was evaluated using samples of Acanthamoeba trophozoites and cysts diluted to various concentrations. A conventional immunochromatographic assay kit with latex labels (LICGA) was also evaluated to determine its sensitivity in detecting Acanthamoeba trophozoites. To check for cross-reactivity, the FICGA was performed by using samples of other common causative pathogens of infectious keratitis, such as Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans. Corneal scrapings from patients with suspected AK were tested with the FICGA kit to detect the presence of Acanthamoeba, and the results were compared with those of real-time PCR. The FICGA kit detected organisms at concentrations as low as 5 trophozoites or 40 cysts per sample. There were no cross-reactivities with other pathogens. The FICGA was approximately 20 times more sensitive than the LICGA for the detection of Acanthamoeba trophozoites. The FICGA kit yielded positive results for all 10 patients, which corresponded well with the real-time PCR results. The FICGA kit demonstrated high sensitivity for the detection of Acanthamoeba and may be useful for the diagnosis of AK. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  14. Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

    Science.gov (United States)

    Toriyama, Koji; Inoue, Tomoyuki; Eguchi, Hiroshi; Hoshi, Saichi; Inoue, Yoshitsugu; Aizawa, Hideki; Miyoshi, Kazutomi; Ohkubo, Michio; Hiwatashi, Eiji; Tachibana, Hiroshi; Ohashi, Yuichi

    2014-01-01

    We developed an immunochromatographic assay kit that uses fluorescent silica nanoparticles bound to anti-Acanthamoeba antibodies (fluorescent immunochromatographic assay [FICGA]) and evaluated its efficacy for the detection of Acanthamoeba and diagnosis of Acanthamoeba keratitis (AK). The sensitivity of the FICGA kit was evaluated using samples of Acanthamoeba trophozoites and cysts diluted to various concentrations. A conventional immunochromatographic assay kit with latex labels (LICGA) was also evaluated to determine its sensitivity in detecting Acanthamoeba trophozoites. To check for cross-reactivity, the FICGA was performed by using samples of other common causative pathogens of infectious keratitis, such as Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans. Corneal scrapings from patients with suspected AK were tested with the FICGA kit to detect the presence of Acanthamoeba, and the results were compared with those of real-time PCR. The FICGA kit detected organisms at concentrations as low as 5 trophozoites or 40 cysts per sample. There were no cross-reactivities with other pathogens. The FICGA was approximately 20 times more sensitive than the LICGA for the detection of Acanthamoeba trophozoites. The FICGA kit yielded positive results for all 10 patients, which corresponded well with the real-time PCR results. The FICGA kit demonstrated high sensitivity for the detection of Acanthamoeba and may be useful for the diagnosis of AK. PMID:25392356

  15. Development of coated particle fuel technology

    International Nuclear Information System (INIS)

    Cho, Moonsung; Kim, B. G.; Kim, D. J.

    2011-06-01

    Ammonia contacting method for prehardenning the surfaces of ADU liquid droplets and the ageing/washing/drying method and equipment for spherical dried-ADU particles were improved and tested with laboratory sacle. After the improvement of fabrication process, the sphericity of UO 2 kernel obtained to 1.1, and the sintered density and O/U ratio of final UO 2 kernel were above 10.60g/cm 3 . 2.01 respectively. Defects of SiC coating layer could be minimized by optimization of gas flow rate. The fracture strength of SiC layer increased from 450 MPa to 530 MPa by controlling the coating defects. An effort was made to develop the fundamental technology for the fuel element compact for use in High Temperature Gas-cooled Reactor(HTGR) through an establishment of fabrication process, required materials and process equipment as well as performing experiments to identify the basic process conditions and optimize them. Thermal load simulation and verification experiments were carried out for an assesment of the design feasibility of the irradiation rod. Out-of-pile testing of irradiation device such as measurement of pressure drop and vibration, endurance test was performed and the validity of its design was confirmed. A fuel performance analysis code, COPA has been developed to calculate the fuel temperature, the failure fractions of coated fuel particles, the release of fission products. The COPA code can be used to evaluate the performance of the high temperature reactor fuel under the reactor operation, irradiation, heating conditions. KAERI participated in the round robin test of IAEA CRP-6 program to characterize the diameter, sphericity, coating thickness, density and anisotropy of coated particles provided by Korea, USA and South Africa. QC technology was established for TRISO-coated fuel particle. A method for accurate measurement of the optical anisotropy factor for PyC layers of coated particles was developed. Technology and inspection procedures for density

  16. Development of a direct PCR assay to detect Taenia multiceps eggs isolated from dog feces.

    Science.gov (United States)

    Wang, Ning; Wang, Yu; Ye, Qinghua; Yang, Yingdong; Wan, Jie; Guo, Cheng; Zhan, Jiafei; Gu, Xiaobin; Lai, Weimin; Xie, Yue; Peng, Xuerong; Yang, Guangyou

    2018-02-15

    Taenia multiceps is a tapeworm that leads to the death of livestock, resulting in major economic losses worldwide. The adult stage of this parasite invades the small intestine of dogs and other canids. In the present study, we developed a direct PCR assay to detect T. multiceps eggs isolated from dog feces to help curb further outbreaks. The genomic DNA was rapidly released using a lysis buffer and the PCR reaction was developed to amplify a 433-bp fragment of the T. multiceps mitochondrial gene encoding NADH dehydrogenase subunit 5 (nad5) from eggs isolated from dog feces. The procedure could be completed within 3 h, including flotation. The sensitivity of the assay was determined by detecting DNA from defined numbers of eggs, and the specificity was determined by detecting DNA from other intestinal tapeworm and roundworm species that commonly infect dogs. In addition, 14 taeniid-positive fecal samples determined by the flotation technique were collected and further evaluated by the regular PCR and our direct PCR. The results showed that the direct PCR developed herein was sensitive enough to detect the DNA from as few as 10 T. multiceps eggs and that no cross-reactions with other tapeworm and roundworm were observed, suggesting its high sensitivity and specificity for T. multiceps detection. Moreover, 14 taeniid-positive samples were screened by the regular PCR and direct PCR, with detection rates of 78.6% and 85.7%, respectively. In conclusion, the direct PCR assay developed in the present study has high sensitivity and specificity to identify T. multiceps eggs isolated from dog feces and therefore could represent an invaluable tool to identify T. multiceps outbreaks and would contribute to future clinical applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Development of Conventional and Real-Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies.

    Science.gov (United States)

    Wong, Samson S Y; Poon, Rosana W S; Chau, Sandy; Wong, Sally C Y; To, Kelvin K W; Cheng, Vincent C C; Fung, Kitty S C; Yuen, K Y

    2015-07-01

    Scabies remains the most prevalent, endemic, and neglected ectoparasitic infestation globally and can cause institutional outbreaks. The sensitivity of routine microscopy for demonstration of Sarcoptes scabiei mites or eggs in skin scrapings is only about 50%. Except for three studies using conventional or two-tube nested PCR on a small number of cases, no systematic study has been performed to improve the laboratory diagnosis of this important infection. We developed a conventional and a real-time quantitative PCR (qPCR) assay based on the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of S. scabiei. The cox1 gene is relatively well conserved, with its sequence having no high levels of similarity to the sequences of other human skin mites, pathogenic zoonotic mites, or common house dust mite species. This mitochondrial gene is also present in large quantities in arthropod cells, potentially improving the sensitivity of a PCR-based assay. In our study, both assays were specific and were more sensitive than microscopy in diagnosing scabies, with positive and negative predictive values of 100%. The S. scabiei DNA copy number in the microscopy-positive specimens was significantly higher than that in the microscopy-negative specimens (median S. scabiei DNA copy number, 3.604 versus 2.457 log10 copies per reaction; P = 0.0213). In the patient with crusted scabies, the qPCR assay performed on lesional skin swabs instead of scrapings revealed that the parasite DNA load took about 2 weeks to become negative after treatment. The utility of using lesional skin swabs as an alternative sample for diagnosis of scabies by PCR should be further evaluated. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  18. Development and validation of a rapid, aldehyde dehydrogenase bright-based cord blood potency assay.

    Science.gov (United States)

    Shoulars, Kevin; Noldner, Pamela; Troy, Jesse D; Cheatham, Lynn; Parrish, Amanda; Page, Kristin; Gentry, Tracy; Balber, Andrew E; Kurtzberg, Joanne

    2016-05-12

    Banked, unrelated umbilical cord blood provides access to hematopoietic stem cell transplantation for patients lacking matched bone marrow donors, yet 10% to 15% of patients experience graft failure or delayed engraftment. This may be due, at least in part, to inadequate potency of the selected cord blood unit (CBU). CBU potency is typically assessed before cryopreservation, neglecting changes in potency occurring during freezing and thawing. Colony-forming units (CFUs) have been previously shown to predict CBU potency, defined as the ability to engraft in patients by day 42 posttransplant. However, the CFU assay is difficult to standardize and requires 2 weeks to perform. Consequently, we developed a rapid multiparameter flow cytometric CBU potency assay that enumerates cells expressing high levels of the enzyme aldehyde dehydrogenase (ALDH bright [ALDH(br)]), along with viable CD45(+) or CD34(+) cell content. These measurements are made on a segment that was attached to a cryopreserved CBU. We validated the assay with prespecified criteria testing accuracy, specificity, repeatability, intermediate precision, and linearity. We then prospectively examined the correlations among ALDH(br), CD34(+), and CFU content of 3908 segments over a 5-year period. ALDH(br) (r = 0.78; 95% confidence interval [CI], 0.76-0.79), but not CD34(+) (r = 0.25; 95% CI, 0.22-0.28), was strongly correlated with CFU content as well as ALDH(br) content of the CBU. These results suggest that the ALDH(br) segment assay (based on unit characteristics measured before release) is a reliable assessment of potency that allows rapid selection and release of CBUs from the cord blood bank to the transplant center for transplantation. © 2016 by The American Society of Hematology.

  19. Development and validation of a rapid, aldehyde dehydrogenase bright–based cord blood potency assay

    Science.gov (United States)

    Noldner, Pamela; Troy, Jesse D.; Cheatham, Lynn; Parrish, Amanda; Page, Kristin; Gentry, Tracy; Balber, Andrew E.; Kurtzberg, Joanne

    2016-01-01

    Banked, unrelated umbilical cord blood provides access to hematopoietic stem cell transplantation for patients lacking matched bone marrow donors, yet 10% to 15% of patients experience graft failure or delayed engraftment. This may be due, at least in part, to inadequate potency of the selected cord blood unit (CBU). CBU potency is typically assessed before cryopreservation, neglecting changes in potency occurring during freezing and thawing. Colony-forming units (CFUs) have been previously shown to predict CBU potency, defined as the ability to engraft in patients by day 42 posttransplant. However, the CFU assay is difficult to standardize and requires 2 weeks to perform. Consequently, we developed a rapid multiparameter flow cytometric CBU potency assay that enumerates cells expressing high levels of the enzyme aldehyde dehydrogenase (ALDH bright [ALDHbr]), along with viable CD45+ or CD34+ cell content. These measurements are made on a segment that was attached to a cryopreserved CBU. We validated the assay with prespecified criteria testing accuracy, specificity, repeatability, intermediate precision, and linearity. We then prospectively examined the correlations among ALDHbr, CD34+, and CFU content of 3908 segments over a 5-year period. ALDHbr (r = 0.78; 95% confidence interval [CI], 0.76-0.79), but not CD34+ (r = 0.25; 95% CI, 0.22-0.28), was strongly correlated with CFU content as well as ALDHbr content of the CBU. These results suggest that the ALDHbr segment assay (based on unit characteristics measured before release) is a reliable assessment of potency that allows rapid selection and release of CBUs from the cord blood bank to the transplant center for transplantation. PMID:26968535

  20. Development of an ATP assay for rapid onboard testing to detect living microorganisms in ballast water

    Science.gov (United States)

    Hyun, Bonggil; Cha, Hyung-Gon; Lee, Nayoung; Yum, Seungshic; Baek, Seung Ho; Shin, Kyoungsoon

    2018-03-01

    Ballast water is a principal pathway for the introduction of pathogens and non-indigenous species to ports worldwide. The International Maritime Organization (IMO) and the United States Coast Guard (USCG) have adopted ballast water management regulations that require, e.g., the installation of shipboard ballast water management systems (BWMS). Rapid and simple analytical methods are needed to monitor whether ballast water disinfection ensures compliance with the discharge standards. In this study laboratory and full scale land-based testing was used to investigate the suitability of an adenosine triphosphate (ATP) assay for quantifying living organisms (≥ 10 and < 50 μm minimum dimension) in ballast water. In laboratory experiments the ATP assay was highly sensitive, with a detection limit of < 5 cells 0.1 mL- 1. Diatom species (Chaetoceros simplex and Skeletonema costatum) had low ATP concentrations compared with dinoflagellate, Raphidophyceae, and Chrysophyceae species. This was because of differences in cell volume, as the ATP concentration increased exponentially with increasing cell volume. Using a regression model between ATP concentration and cell volume, an estimated the pass and fail ATP concentration in this study (788-98,610 pg mL- 1) was developed for the discharge of ballast water. In land-based testing the ATP assay also showed a good correlation with the presence of living natural plankton cells in control samples, but the ATP concentration (137 pg mL- 1) was much lower than the ATP guideline. The low ATP concentration in natural plankton cells may reflect a decline in their biological activity because of extended exposure to dark conditions. Although our results need further validation, the ATP assay is a suitable tool for monitoring compliance of ballast water treatment.

  1. Development of the NBT assay as a marker of sperm oxidative stress.

    Science.gov (United States)

    Tunc, Ozlem; Thompson, Jeremy; Tremellen, Kelton

    2010-02-01

    Oxidative stress is a well-established cause of male infertility, with reactive oxygen species (ROS) causing infertility principally by impairing sperm motility and DNA integrity. Currently, most clinics do not test their infertile patients for the presence of oxidative stress because the available tests are expensive or difficult to perform. As antioxidant therapy may improve sperm DNA integrity and pregnancy outcomes, it has become apparent that there is an unmet clinical need for an inexpensive and easy-to-perform assay to identify sperm oxidative stress. The aim of this study was to develop a standardized protocol for performing a photometric nitro blue tetrazolium (NBT) assay for the measurement of seminal ROS production via production of coloured formazan, whilst correlating these results with impaired sperm function (motility and DNA integrity). Semen samples from 21 fertile and 36 male aetiology infertile men were assessed for ROS production (NBT assay), sperm DNA integrity (TUNEL), apoptosis (Annexin V) and sperm motility. Infertile men's semen contained on average fourfold higher levels of ROS than fertile men. The production of ROS by sperm was positively correlated with sperm DNA fragmentation and apoptosis, whilst being negatively correlated with sperm motility. Receiver-operating characteristic plot analysis established a cut-off point of 24 microg formazan/10(7) sperm having a sensitivity of 91.7% and a specificity of 81% for determining the fertility status of an individual. This study has been successful in establishing a standardized protocol for performing a photometric seminal NBT assay that has significant clinical utility in identifying men with impaired fertility because of oxidative stress.

  2. Status of SOFCo SOFC technology development

    Energy Technology Data Exchange (ETDEWEB)

    Privette, R.; Perna, M.A.; Kneidel, K. [SOFCo, Alliance, OH (United States)] [and others

    1996-12-31

    SOFCo, a Babcock & Wilcox/Ceramatec Research & Development Limited Partnership, is a collaborative research and development venture to develop technologies related to planar, solid-oxide fuel cells (SOFCs). SOFCo has successfully demonstrated a kW-class, solid-oxide fuel cell module operating on pipeline natural gas. The SOFC system design integrates the air preheater and the fuel processor with the fuel cell stacks into a compact test unit; this is the platform for multi-kW modules. The cells, made of tape-cast zirconia electrolyte and conventional electrode materials, exhibit excel lent stability in single-cell tests approaching 40,000 hours of operation. Stack tests using 10-cm and 15-cm cells with ceramic interconnects also show good performance and stability in tests for many thousands of hours.

  3. Development of membrane technology in BARC

    International Nuclear Information System (INIS)

    Misra, B.M.

    2003-01-01

    BARC has been engaged in research and development work on pressure-driven membrane technology from laboratory to pilot plant scale and its commercial scale deployment, for sea and brackish water desalination into potable water, effluent water treatment and water reuse and in various industrial separations including decontamination of radioactive liquid effluents for their safe disposal into the environment. This paper gives a brief description of pressure-driven membrane processes, reverse osmosis, nano filtration, ultrafiltration and micro filtration. Selection of polymeric candidate materials, preparation of semi-permeable membranes and their characterization has been discussed. Various applications of these processes conducted on pilot plant scale have been presented. Large scale deployment of membrane processes for sea water desalination has been indicated. Research and development at BARC has thus resulted in the indigenous development of membrane processes for commercial scale operation. (author)

  4. Current developments in TREAT hodoscope technology

    International Nuclear Information System (INIS)

    De Volpi, A.

    1975-01-01

    The development of fuel motion monitoring is traced from its inception through present operation and into future programs. After noting the role of fuel motion studies in terms of safety assurance for the LMFBR, the history of in-pile fuel monitoring is reviewed. The operational record of the present TREAT fast neutron hodoscope is summarized with attention to various performance features. Development plans for the TREAT hodoscope are described in some detail. Application of the hodoscope has been considered for eight safety facilities other than TREAT. In addition, there is a possible role for fuel monitoring techniques to be extended to real-time ex-vessel core surveillance in operating reactors. Certain intrinsic strengths of the hodoscope technique for material monitoring are identified. The pattern of development may be characterized as an adaptation of several technologies to fit available requirements and resources

  5. Development of environmental radiation control technology

    International Nuclear Information System (INIS)

    Han, M. H.; Kim, E. H.; Keum, D. K.; Kang, M. J.; Jang, B. W.

    2010-04-01

    The objectives of the study are to development of an urban atmospheric dispersion model and data assimilation technique for improving the reliability, to develop the technology for assessing the radiation impact to biota and the surface water transport model, to develop the analytical techniques for the indicator radionuclides on decommissioning of nuclear facilities and nuclear waste disposal sites and to assess of the national environmental radiation impact and establish the optimum management bases of natural radiation. The obtained results might be used; for assessing the radiological effects due to and radiological incident in an urban area, for assessing radiation doses on biota for the environmental protection from ionizing radiation with the application of new concept of the ICP new recommendation, for analyzing the indicator radionuclides on decommissioning of nuclear facilities and nuclear waste disposal sites, and for providing the natural radionuclide database of Korea to international organizations such as UNSCEAR. It can be used for emphasizing relative nuclear safety

  6. Development of spent fuel remote handling technology

    Energy Technology Data Exchange (ETDEWEB)

    Park, B. S.; Yoon, J. S.; Hong, H. D. (and others)

    2007-02-15

    In this research, the remote handling technology was developed for the ACP application. The ACP gives a possible solution to reduce the rapidly cumulative amount of spent fuels generated from the nuclear power plants in Korea. The remote technologies developed in this work are a slitting device, a voloxidizer, a modified telescopic servo manipulator and a digital mock-up. A slitting device was developed to declad the spent fuel rod-cuts and collect the spent fuel UO{sub 2} pellets. A voloxidizer was developed to convert the spent fuel UO{sub 2} pellets obtained from the slitting process in to U{sub 3}O{sub 8} powder. Experiments were performed to test the capabilities and remote operation of the developed slitting device and voloxidizer by using simulated rod-cuts and fuel in the ACP hot cell. A telescopic servo manipulator was redesigned and manufactured improving the structure of the prototype. This servo manipulator was installed in the ACP hot cell, and the target module for maintenance of the process equipment was selected. The optimal procedures for remote operation were made through the maintenance tests by using the servo manipulator. The ACP digital mockup in a virtual environment was established to secure a reliability and safety of remote operation and maintenance. The simulation for the remote operation and maintenance was implemented and the operability was analyzed. A digital mockup about the preliminary conceptual design of an enginnering-scale ACP was established, and an analysis about a scale of facility and remote handling was accomplished. The real-time diagnostic technique was developed to detect the possible fault accidents of the slitting device. An assessment of radiation effect for various sensors was also conducted in the radiation environment.

  7. Development of Recombinase Polymerase Amplification Assays for Detection of Orientia tsutsugamushi or Rickettsia typhi.

    Directory of Open Access Journals (Sweden)

    Chien-Chung Chao

    Full Text Available Sensitive, specific and rapid diagnostic tests for the detection of Orientia tsutsugamushi (O. tsutsugamushi and Rickettsia typhi (R. typhi, the causative agents of scrub typhus and murine typhus, respectively, are necessary to accurately and promptly diagnose patients and ensure that they receive proper treatment. Recombinase polymerase amplification (RPA assays using a lateral flow test (RPA-nfo and real-time fluorescent detection (RPA-exo were developed targeting the 47-kDa gene of O. tsutsugamushi or 17 kDa gene of R. typhi. The RPA assay was capable of detecting O. tsutsugamushi or R. typhi at levels comparable to that of the quantitative PCR method. Both the RPA-nfo and RPA-exo methods performed similarly with regards to sensitivity when detecting the 17 kDa gene of R. typhi. On the contrary, RPA-exo performed better than RPA-nfo in detecting the 47 kDa gene of O. tsutsugamushi. The clinical performance of the O. tsutsugamushi RPA assay was evaluated using either human patient samples or infected mouse samples. Eight out of ten PCR confirmed positives were determined positive by RPA, and all PCR confirmed negative samples were negative by RPA. Similar results were obtained for R. typhi spiked patient sera. The assays were able to differentiate O. tsutsugamushi and R. typhi from other phylogenetically related bacteria as well as mouse and human DNA. Furthermore, the RPA-nfo reaction was completed in 20 minutes at 37°C followed by a 10 minute incubation at room temperature for development of an immunochromatographic strip. The RPA-exo reaction was completed in 20 minutes at 39°C. The implementation of a cross contamination proof cassette to detect the RPA-nfo fluorescent amplicons provided an alternative to regular lateral flow detection strips, which are more prone to cross contamination. The RPA assays provide a highly time-efficient, sensitive and specific alternative to other methods for diagnosing scrub typhus or murine typhus.

  8. Technology Development for Cosmic Microwave Background Cosmology

    Science.gov (United States)

    Munson, Charles D.

    2017-05-01

    The Cosmic Microwave Background (CMB) offers a unique window into the early universe by probing thermal radiation remaining from the big bang. Due to its low temperature and bright foregrounds, its thorough characterization requires technological advancement beyond the current state-of-the-art. In this thesis, I present the development and fabrication of novel metamaterial silicon optics to improve the sensitivity of current and future CMB telescopes. By machining subwavelength features into the silicon surfaces, traditional antireflection coatings can be replaced by all-silicon metamaterials that significantly reduce reflections over previous approaches. I discuss the design of these structured surfaces and the design and construction of a sophisticated fabrication facility necessary to implement this technology on large diameter (30+ cm) lenses for the Atacama Cosmology Telescope Polarization project (ACTPol). I then apply this metamaterial technology to the development of improved free-space filters for millimeter and sub-millimeter wavelength imaging (focusing specifically on blocking infrared radiation, necessary for current cryogenic detector systems). This produces a highly effective infrared-blocking filter, blocking over 99% of the incident power from a 300 K blackbody while maintaining transmission of better than 99% in a target CMB observing band (between 70 and 170 GHz). I conclude with a discussion of the development of a real-space simulation framework to assist in better understanding current CMB results and forecasting for future experiments. By taking a CMB realization and adding to it accurate real-space modeling of the Sunyaev-Zel'dovich effect and weak lensing distortions (introduced by galaxy clusters), a better understanding of the impacts of large scale structure on the CMB can be obtained.

  9. Development of Advanced Ceramic Manufacturing Technology

    Energy Technology Data Exchange (ETDEWEB)

    Pujari, V.K.

    2001-04-05

    Advanced structural ceramics are enabling materials for new transportation engine systems that have the potential for significantly reducing energy consumption and pollution in automobiles and heavy vehicles. Ceramic component reliability and performance have been demonstrated in previous U.S. DOE initiatives, but high manufacturing cost was recognized as a major barrier to commercialization. Norton Advanced Ceramics (NAC), a division of Saint-Gobain Industrial Ceramics, Inc. (SGIC), was selected to perform a major Advanced Ceramics Manufacturing Technology (ACMT) Program. The overall objectives of NAC's program were to design, develop, and demonstrate advanced manufacturing technology for the production of ceramic exhaust valves for diesel engines. The specific objectives were (1) to reduce the manufacturing cost by an order of magnitude, (2) to develop and demonstrate process capability and reproducibility, and (3) to validate ceramic valve performance, durability, and reliability. The program was divided into four major tasks: Component Design and Specification, Component Manufacturing Technology Development, Inspection and Testing, and Process Demonstration. A high-power diesel engine valve for the DDC Series 149 engine was chosen as the demonstration part for this program. This was determined to be an ideal component type to demonstrate cost-effective process enhancements, the beneficial impact of advanced ceramics on transportation systems, and near-term commercialization potential. The baseline valve material was NAC's NT451 SiAION. It was replaced, later in the program, by an alternate silicon nitride composition (NT551), which utilized a lower cost raw material and a simplified powder-processing approach. The material specifications were defined based on DDC's engine requirements, and the initial and final component design tasks were completed.

  10. Developments in rubber technology 2 synthetic rubbers

    CERN Document Server

    Lee, K

    1981-01-01

    This book is intended for those people who have a knowledge or understanding of rubber materials and processes but who wish to update their knowledge. It should be read in conjunction with Developments in Rubber Technology-l as that volume discussed developments in natural rubber and selected special purpose synthetic rubbers as well as additives. The authors have been selected for their expertise in each particular field and we, as editors, would like to express our appreciation to the individual authors and also to their companies. Such a book would be impossible to produce without such active cooperation as we have received. Volumes 1 and 2 of Developments in Rubber Technology cover rubbers which are processed and vulcanised in the traditional manner. It is appreciated that the omission of non-vulcanised rubber materials (the so­ called thermoplastic elastomers) will be unwelcome to many readers but it is intended, because of the size of the subject, to cover these materials in a subsequent volume. A.W. K...

  11. Development of inspection safety evaluation technology

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Seok Chul; Yoon, Yeo Chang; Kim, Jong Soo; Lee, Tae Young; Kim, Chang Ryol; Lee, Hyung Sub; Kim, Jong Soo

    1995-12-01

    The purpose of this project is to protection nation inspector`s over exposure from radiation that can be occurred by inspection activity at nuclear facilities and its environment, and to ensure the safety of inspection activity at the nuclear facilities. To effectively carry out the domestic inspection task to be enforced from 1996, the evaluation for special radiation exposure rate of nuclear facilities, air and surface contamination level, and measurement and monitoring of water contamination level were made to determine whether these measured values exceeded permissible limitations, and to protect the inspector`s over exposure from radiation at domestic nuclear facilities. Management of inspector`s exposure was carried out under assistance of the Department of Health Physics. Performance tests of two gamma detectors, one neutron detector, alpha and beta detector, and gamma spectroscopy analyzer were carried out to control dose on extremity, the characteristic test for extremity dosimeter was carried out and the theoretical calculation of gamma dose conversion factors based on ANSI N13.32 standard was performed. Under the 93+2 program, IAEA began to recognize the necessity of environmental observation technology development of air-borne particulates travelled from long distance location. Associated with the necessity of this technology development, a proposal of international joint research for development of the special radiation measurement and analysis has been prepared. (author). 21 tabs., 24 figs., 20 refs.

  12. Technology development for high temperature logging tools

    Energy Technology Data Exchange (ETDEWEB)

    Veneruso, A.F.; Coquat, J.A.

    1979-01-01

    A set of prototype, high temperature logging tools (temperature, pressure and flow) were tested successfully to temperatures up to 275/sup 0/C in a Union geothermal well during November 1978 as part of the Geothermal Logging Instrumentation Development Program. This program is being conducted by Sandia Laboratories for the Department of Energy's Division of Geothermal Energy. The progress and plans of this industry based program to develop and apply the high temperature instrumentation technology needed to make reliable geothermal borehole measurements are described. Specifically, this program is upgrading existing sondes for improved high temperature performance, as well as applying new materials (elastomers, polymers, metals and ceramics) and developing component technology such as high temperature cables, cableheads and electronics to make borehole measurements such as formation temperature, flow rate, high resolution pressure and fracture mapping. In order to satisfy critical existing needs, the near term goal is for operation up to 275/sup 0/C and 7000 psi by the end of FY80. The long term goal is for operation up to 350/sup 0/C and 20,000 psi by the end of FY84.

  13. Development of Essential Technology for VHTR

    International Nuclear Information System (INIS)

    Kim, Yong Wan; Koo, G. H.; Kim, D. H.

    2009-04-01

    The research tasks performed in this project can be classified into five categories; high temperature material of VHTR reactor and components for hydrogen production, the nuclear graphite for the core material, the essential technologies for VHTR components, Process Heat Exchanger (PHE) fabrication, and gas loop for PHE verification tests. Research tasks on high temperature materials of VHTR reactor and components include creep properties of super alloy for high temperature components, properties of a modified 9Cr-1Mo alloy, fabrication and properties of in-core ceramic composites, and corrosion properties of the materials for the sulfuric acid decomposer. The technologies of graphitization evaluation, nondestructive defect detection, and impurity analysis were developed in field of nuclear graphites. The properties of graphites were evaluated by tests using small specimen test. The abroad status of graphite machining was reviewed. Review about the status of VHTR components, structural sizing and analysis for hot gas duct, thermal sizing of IHX were performed in the field of the essential technologies for VHTR components. The surface modification process with ion beam mixing was optimized and evaluated for the fabrication of process heat exchanger (PHE). The secondary sulfuric acid loop was designed and constructed in the gas loop. The lab-scale PHE test was performed in the gas loop. In addition, the conceptual design of the mid-size helium loop was performed in the next stage of this project

  14. Wind Energy Workforce Development: Engineering, Science, & Technology

    Energy Technology Data Exchange (ETDEWEB)

    Lesieutre, George A.; Stewart, Susan W.; Bridgen, Marc

    2013-03-29

    Broadly, this project involved the development and delivery of a new curriculum in wind energy engineering at the Pennsylvania State University; this includes enhancement of the Renewable Energy program at the Pennsylvania College of Technology. The new curricula at Penn State includes addition of wind energy-focused material in more than five existing courses in aerospace engineering, mechanical engineering, engineering science and mechanics and energy engineering, as well as three new online graduate courses. The online graduate courses represent a stand-alone Graduate Certificate in Wind Energy, and provide the core of a Wind Energy Option in an online intercollege professional Masters degree in Renewable Energy and Sustainability Systems. The Pennsylvania College of Technology erected a 10 kilowatt Xzeres wind turbine that is dedicated to educating the renewable energy workforce. The entire construction process was incorporated into the Renewable Energy A.A.S. degree program, the Building Science and Sustainable Design B.S. program, and other construction-related coursework throughout the School of Construction and Design Technologies. Follow-on outcomes include additional non-credit opportunities as well as secondary school career readiness events, community outreach activities, and public awareness postings.

  15. High technology in developing countries: Analysis of technology strategy, technology transfer, and success factors in the aircraft industry

    OpenAIRE

    Steenhuis, H.J.; de Bruijn, E.J.

    2004-01-01

    Economical development is highly related to technological development. It is therefore not surprising that many of the industrially developing nations follow explicit strategies to increase their technological competence level. Industrially developing countries may even pursue a strategy of developing high technology competencies. This paper analysis the strategies of some developing countries in a particular high technology industry: the aircraft manufacturing industry. The focus is on Brazi...

  16. Impaired DNA repair as assessed by the ``comet`` assay in patients developing thyroid carcinoma after radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Leprat, F.; Sarasin, A.; Suarez, H.G. [Institut de Recherches sur le Cancer, 94 - Villesuif (France); Leprat, F.; Alapetite, C.; Rosselli, F.; Ridet, A.; Moustacch, E.; Alapetite, C. [Institut Curie, 75 - Paris (France); Schlumberger, M. [Institut Gustave Roussy, 94 - Villejuif (France)

    1997-03-01

    A defective cellular response to DNA lesions induced be genotoxic agents may be associated to an increased cancer proneness. This has been clearly identified in some rare but extensively studied genetic diseases such as xeroderma pigmentosum (XP), ataxia telangiectasia (AT) and Fanconi anemia (FA). In practical oncology, most patients receive genotoxic therapeutic agents and the presence of so far unidentified sensitive genotypes could account for an increased susceptibility to cancer in a subgroup of exposed patients. The thyroid gland of children is especially sensitive to the carcinogenic effect of ionizing radiation. Evidence for risk is reported even at doses as low as 0.1 Gy, and the excess relative risk to develop a thyroid tumor following a radiation dose of 1 Gy in childhood is of 7.7 [l]. In order to determine if a defect in repair of DNA strand breaks could be involved, as an early step, in the development of secondary thyroid tumors after radiotherapy, we examined, using the alkaline single cell gel electrophoresis assay (SCGE or `comet`), the response to in vitro {gamma}-rays exposure of lymphocytes of a small group of patients who developed thyroid carcinoma after radiotherapy for a primary tumor. Because of its practical advantages, the alkaline comet assay offers the opportunity to question the role of DNA strand beaks rejoining capacity of the individual in the radiation induced carcinogenesis of thyroid tumors. This preliminary study of a small group of patients with therapeutic irradiation at childhood for a primary tumor indicates that, at the time of blood sampling, lymphocytes of some of these patients demonstrated reduced rejoining capacity. These results suggest that the comet assay might help to distinguish a subgroup of individuals at risk for radiation induced genomic instability and encourage further investigation. (authors)

  17. Lessons Learned: Transfer of the High-Definition Circulating Tumor Cell Assay Platform to Development as a Commercialized Clinical Assay Platform.

    Science.gov (United States)

    Kuhn, P; Keating, S M; Baxter, G T; Thomas, K; Kolatkar, A; Sigman, C C

    2017-11-01

    Planning and transfer of a new technology platform developed in an academic setting to a start-up company for medical diagnostic product development may appear daunting and costly in terms of complexity, time, and resources. In this review we outline the key steps taken and lessons learned when a technology platform developed in an academic setting was transferred to a start-up company for medical diagnostic product development in the interest of elucidating development toolkits for academic groups and small start-up companies starting on the path to commercialization and regulatory approval. © 2017, The American Society for Clinical Pharmacology and Therapeutics.

  18. Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.

    Science.gov (United States)

    García, Alfredo; Martínez, Remigio; Benitez-Medina, José Manuel; Risco, David; Garcia, Waldo Luis; Rey, Joaquín; Alonso, Juan Manuel; Hermoso de Mendoza, Javier

    2013-01-01

    Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.

  19. NASA's Exploration Technology Development Program Energy Storage Project Battery Technology Development

    Science.gov (United States)

    Reid, Concha M.; Miller, Thomas B.; Mercer, Carolyn R.; Jankovsky, Amy L.

    2010-01-01

    Technical Interchange Meeting was held at Saft America s Research and Development facility in Cockeysville, Maryland on Sept 28th-29th, 2010. The meeting was attended by Saft, contractors who are developing battery component materials under contracts awarded through a NASA Research Announcement (NRA), and NASA. This briefing presents an overview of the components being developed by the contractor attendees for the NASA s High Energy (HE) and Ultra High Energy (UHE) cells. The transition of the advanced lithium-ion cell development project at NASA from the Exploration Technology Development Program Energy Storage Project to the Enabling Technology Development and Demonstration High Efficiency Space Power Systems Project, changes to deliverable hardware and schedule due to a reduced budget, and our roadmap to develop cells and provide periodic off-ramps for cell technology for demonstrations are discussed. This meeting gave the materials and cell developers the opportunity to discuss the intricacies of their materials and determine strategies to address any particulars of the technology.

  20. Development of loop-mediated isothermal amplification and PCR assays for rapid and simple detection of Campylobacter fetus subsp. venerealis.

    Science.gov (United States)

    Yamazaki, Wataru; Taguchi, Masumi; Misawa, Naoaki

    2010-07-01

    Campylobacter fetus is divided into CFV and CFF. Because CFV causes bovine genital campylobacteriosis, differentiation of the two subspecies is essential to the implementation of efficient CFV control and eradication programs. We have developed LAMP and duplex PCR assays for rapid and simple detection of CFV. The LAMP assay correctly detected 7 CFV strains and did not detect 53 CFF, 35 non-fetus Campylobacter and 25 non-Campylobacter strains. The PCR assay successfully differentiated the two subspecies. The LAMP and PCR assays were faster than conventional biochemical assays, requiring for detection less than 50 min and less than 4 hr, respectively, from the beginning of DNA extraction from a single colony on blood agar to final determination. Our LAMP and PCR assays are rapid and practical tools for detection of CFV.

  1. Development of a PCR assay suitable for Campylobacter spp. mass screening programs in broiler production

    DEFF Research Database (Denmark)

    Bang, Dang Duong; Pedersen, Karl; Madsen, Mogens

    2001-01-01

    culture techniques since 1998. However, using conventional culture methods is time consuming and laborious, and therefore a Polymerase Chain Reaction (PCR) Campylobacter detection assay suitable for mass screening of cloacal swab samples from broilers was developed. By comparing the PCR detection...... with conventional culture methods, significantly more samples were found positive for Campylobacter with the PCR method. The PCR method is rapid, sensitive and suitable for mass screening for Campylobacter in poultry. Using this PCR method Campylobacter can be detected within 15 h. Notably, the method can...... be applied to detect Campylobacter directly from chicken feces at the species level....

  2. Development of nuclear fuel cycle technologies

    International Nuclear Information System (INIS)

    Suzuoki, Akira; Matsumoto, Takashi; Suzuki, Kazumichi; Kawamura, Fumio

    1995-01-01

    In the long term plan for atomic energy that the Atomic Energy Commission decided the other day, the necessity of the technical development for establishing full scale fuel cycle for future was emphasized. Hitachi Ltd. has engaged in technical development and facility construction in the fields of uranium enrichment, MOX fuel fabrication, spent fuel reprocessing and so on. In uranium enrichment, it took part in the development of centrifuge process centering around Power Reactor and Nuclear Fuel Development Corporation (PNC), and took its share in the construction of the Rokkasho uranium enrichment plant of Japan Nuclear Fuel Service Co., Ltd. Also it cooperates with Laser Enrichment Technology Research Association. In Mox fuel fabrication, it took part in the construction of the facilities for Monju plutonium fuel production of PNC, for pellet production, fabrication and assembling processes. In spent fuel reprocessing, it cooperated with the technical development of maintenance and repair of Tokai reprocessing plant of PNC, and the construction of spent fuel stores in Rokkasho reprocessing plant is advanced. The centrifuge process and the atomic laser process of uranium enrichment are explained. The high reliability of spent fuel reprocessing plants and the advancement of spent fuel reprocessing process are reported. Hitachi Ltd. Intends to exert efforts for the technical development to establish nuclear fuel cycle which increases the importance hereafter. (K.I.)

  3. Development of clean environment conservation technology by radiation

    International Nuclear Information System (INIS)

    Lee, Myunjoo; Kim, Tak Hyun; Jung, In Ha

    2012-04-01

    This report is aim to develop the technology for environmental conservation by radiation. It is consisted of two research parts. One is development of wastewater disinfection technology by radiation and the other is development of livestock waste treatment technology by radiation. For the development of wastewater disinfection technology disinfect ion process, technology for treatment of toxic organic chemicals and assessment of ecological toxicity, technology for treatment of endocrine disrupting chemicals and assessment of genetic safety were developed. For the development of livestock waste treatment technology, process for simultaneous removal of nutrients, technology for disinfection and quality enhancement of livestock waste compost, technology for reduction of composting periods, monitoring of toxic organic compounds, pretreatment technology for organic toxic chemicals and enhancement of biological treatment efficiencies were developed. Based on basic research, advanced livestock wastewater treatment process using radiation was established

  4. Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species.

    Science.gov (United States)

    Gopaul, Krishna K; Sells, Jessica; Lee, Robin; Beckstrom-Sternberg, Stephen M; Foster, Jeffrey T; Whatmore, Adrian M

    2014-12-11

    The zoonosis brucellosis causes economically significant reproductive problems in livestock and potentially debilitating disease of humans. Although the causative agent, organisms from the genus Brucella, can be differentiated into a number of species based on phenotypic characteristics, there are also significant differences in genotype that are concordant with individual species. This paper describes the development of a five target multiplex assay to identify five terrestrial Brucella species using real-time polymerase chain reaction (PCR) and subsequent high resolution melt curve analysis. This technology offers a robust and cost effective alternative to previously described hydrolysis-probe Single Nucleotide Polymorphism (SNP)-based species defining assays. Through the use of Brucella whole genome sequencing five species defining SNPs were identified. Individual HRM assays were developed to these target these changes and, following optimisation of primer concentrations, it was possible to multiplex all five assays in a single tube. In a validation exercise using a panel of 135 Brucella strains of terrestrial and marine origin, it was possible to distinguish the five target species from the other species within this panel. The HRM multiplex offers a number of diagnostic advantages over previously described SNP-based typing approaches. Further, and uniquely for HRM, the successful multiplexing of five assays in a single tube allowing differentiation of five Brucella species in the diagnostic laboratory in a cost-effective and timely manner is described. However there are possible limitations to using this platform on DNA extractions direct from clinical material.

  5. The impact of assay technology as applied to safety assessment in reducing compound attrition in drug discovery.

    Science.gov (United States)

    Thomas, Craig E; Will, Yvonne

    2012-02-01

    Attrition in the drug industry due to safety findings remains high and requires a shift in the current safety testing paradigm. Many companies are now positioning safety assessment at each stage of the drug development process, including discovery, where an early perspective on potential safety issues is sought, often at chemical scaffold level, using a variety of emerging technologies. Given the lengthy development time frames of drugs in the pharmaceutical industry, the authors believe that the impact of new technologies on attrition is best measured as a function of the quality and timeliness of candidate compounds entering development. The authors provide an overview of in silico and in vitro models, as well as more complex approaches such as 'omics,' and where they are best positioned within the drug discovery process. It is important to take away that not all technologies should be applied to all projects. Technologies vary widely in their validation state, throughput and cost. A thoughtful combination of validated and emerging technologies is crucial in identifying the most promising candidates to move to proof-of-concept testing in humans. In spite of the challenges inherent in applying new technologies to drug discovery, the successes and recognition that we cannot continue to rely on safety assessment practices used for decades have led to rather dramatic strategy shifts and fostered partnerships across government agencies and industry. We are optimistic that these efforts will ultimately benefit patients by delivering effective and safe medications in a timely fashion.

  6. Trends in the development of confectionery technology

    Directory of Open Access Journals (Sweden)

    Z. A. Kanarskaya

    2016-01-01

    Full Text Available Nutrition is one of the main factors that have an enormous impact on the health, performance, creativity, activity and life expectancy, as all essential nutrients enter the body is food. Nutrients are transformed by metabolism in the structural elements of the cell, providing the necessary human plastic material and energy. The traditional source of important nutrients for the human body are the pastries, which are in our population is very popular. However, confectionery products is inherent drawback. Excessive consumption of flour confectionery goods violated balanced diets on nutrients and energy value. This is due to a high fat, carbohydrates, and low enough in some cases, complete absence of fiber, minerals and vitamins. In connection with the formation of the system of healthy nutrition of the population requires the development of production technology of confectionery products with the introduction in their structure of functional ingredients, do not change the organoleptic properties of the product, but can help reduce caloric properties of food and the taste of Tatar national. The purpose of this survey – to identify trends in the development of confectionery technology. This review shows that in confectionery production technology to reduce calories and increase the biological value of the replacement trend in the formulations of wheat flour on chickpeas, flax, triticale, sorghum, rice, barley, corn, bird cherry. It is noted that in the recipes for cakes confectionery introduced from non-traditional raw materials containing dietary fiber. It determined to be a gluten-free medical and functional confectionery products for people with celiac disease.

  7. Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y.

    Science.gov (United States)

    Empey Campora, Cara; Hokama, Yoshitsugi; Yabusaki, Kenichi; Isobe, Minoru

    2008-01-01

    A sandwich enzyme-linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G-horseradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined.

  8. Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis.

    Science.gov (United States)

    Tardif, Keith D; Schlaberg, Robert

    2017-05-01

    Identification of Candida species by traditional methods can be time-consuming and have limited analytical sensitivity. We developed a multiplex real-time PCR assay for detection and differentiation of Candida species causing vulvovaginal candidiasis (VVC). Overall, this PCR assay is a powerful diagnostic tool offering superior accuracy, sensitivity, and specificity. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. Technology development needs summary, FY 1995

    International Nuclear Information System (INIS)

    1994-03-01

    Historic activities of DOE during the period of nuclear weapons development, and disposal practices of that time, resulted in the discharge of chemical and radioactive materials to the environment at many DOE facilities and sites. DOE has now focused a major technical effort on mitigating the effects of those discharges through an environmental restoration program. Since this could lead to prohibitive costs if conventional technology is applied for remedial action, a national program will be initiated to develop and demonstrate faster, better, cheaper, and safer means of restoring the DOE sites to conditions that will meet state and federal environment regulations. Key elements of the initiative are the Integrated Programs and Integrated Demonstrations, which work together to identify possible solutions to major environmental problems. Needed statements are given for the following programs: mixed waste landfill, uranium in soils, VOC-arid, decontamination and decommissioning of facilities, buried waste, characterization/monitoring/sensor technology, mixed waste, in situ remediation, efficient separations/processing, minimum additive waste stabilization, supercritical water oxidation. A section on how to get involved is included

  10. Current Status of VHTR Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    David Petti; Hans Gougar; Richard Wright; William Windes; Steve Herring; Richard Schultz; Paul Humrickhouse

    2010-10-01

    Abstract – High Temperature Gas-cooled Reactors (HTGRs) featuring particle fuel reached the stage of commercial deployment in the mid-1980s with the Fort St.Vrain and Thorium HochTemperatur Reaktor feeding electricity to the grids in the United States and West Germany, respectively. The technology was then adopted by Japan and China with the operation of the High Temperature Test Reactor in Oarai, Japan and the High Temperature Reactor (HTR-10) in China. Increasing the outlet temperature of the HTGR to even higher temperatures above 900°C will improve the thermodynamic efficiency of the system and enable application of a new class of gas reactor, the very high temperature reactor, to provide process heat, electricity, and hydrogen to chemical industries with the attendant benefits of improved energy security and reduced CO2 emissions. However, the increase in coolant outlet temperature presents a number of technical challenges associated with fuel, materials, power conversion, and analysis methods for the reactor and hydrogen production. The U.S. Department of Energy is sponsoring a broad program of research and development with a goal of addressing the technical challenges over a broad range of outlet temperatures as part of the Next Generation Nuclear Plant Project. This paper describes the research and development activities that are currently underway to realize the technologies needed for an HTGR that features outlet temperatures of 750 to 950°C.

  11. Technology development needs summary, FY 1995

    Energy Technology Data Exchange (ETDEWEB)

    1994-03-01

    Historic activities of DOE during the period of nuclear weapons development, and disposal practices of that time, resulted in the discharge of chemical and radioactive materials to the environment at many DOE facilities and sites. DOE has now focused a major technical effort on mitigating the effects of those discharges through an environmental restoration program. Since this could lead to prohibitive costs if conventional technology is applied for remedial action, a national program will be initiated to develop and demonstrate faster, better, cheaper, and safer means of restoring the DOE sites to conditions that will meet state and federal environment regulations. Key elements of the initiative are the Integrated Programs and Integrated Demonstrations, which work together to identify possible solutions to major environmental problems. Needed statements are given for the following programs: mixed waste landfill, uranium in soils, VOC-arid, decontamination and decommissioning of facilities, buried waste, characterization/monitoring/sensor technology, mixed waste, in situ remediation, efficient separations/processing, minimum additive waste stabilization, supercritical water oxidation. A section on how to get involved is included.

  12. Photonics technology development for optical fuzing

    Science.gov (United States)

    Geib, K. M.; Serkland, D. K.; Keeler, G. A.; Peake, G. M.; Mar, A.; von der Lippe, C. M.; Liu, J. J.

    2005-09-01

    This paper describes the photonic component development taking place at Sandia National Laboratories, ARDEC and the Army Research Laboratory in support of an effort to develop a robust, compact, and affordable photonic proximity sensor for munitions fuzing applications. Successful implementation of this sensor will provide a new capability for direct fire applications. The technologies under investigation for the optical fuze design covered in this paper are vertical-cavity surface-emitting lasers (VCSELs), vertical-external-cavity surface-emitting lasers (VECSELs), integrated resonant-cavity photodetectors (RCPDs), and refractive micro-optics. The culmination of this work will be low cost, robust, fully integrated, g-hardened components suitable for proximity fuzing applications. The use of advanced photonic components will enable replacement of costly assemblies that employ discrete lasers, photodetectors, and bulk optics. The integrated devices will be mass produced and impart huge savings for a variety of Army applications. The specific application under investigation is for gun-fired munitions. Nevertheless, numerous civilian uses exist for this proximity sensor in automotive, robotics and aerospace applications. This technology is also applicable to robotic ladar and short-range 3-D imaging.

  13. Guiding recombinant antivenom development by omics technologies

    DEFF Research Database (Denmark)

    Laustsen, Andreas Hougaard

    2017-01-01

    , endogenous animal proteins with toxin-neutralizing capabilities, and recombinant monoclonal antibodies. Harnessing either of these approaches, antivenom development may benefit from an in-depth understanding of venom compositions and the medical importance of individual venom toxins. Focus is thus also...... directed towards the different omics technologies (particularly venomics, antivenomics, and toxicovenomics) that are being used to uncover novel animal toxins, shed light on venom complexity, and provide directions for how to determine the medical relevance of individual toxins within whole venoms. Finally......In this review, the different approaches that have been employed with the aim of developing novel antivenoms against animal envenomings are presented and discussed. Reported efforts have focused on the use of innovative immunization strategies, small molecule inhibitors against enzymatic toxins...

  14. New developments in nuclear medicine technology

    International Nuclear Information System (INIS)

    Ziegler, S.I.; Pichler, B.J.

    2000-01-01

    During the past few years, there have been new impulses in the development of a number of technologies employed in Nuclear Medicine imaging. These include new scintillation materials, the way of detecting the scintillation light, and completely novel methods to detect gamma rays by means of semiconductor detectors. In addition to combined instrumentation that can be used for SPECT and PET, already in clinical use, combined scintigraphic and anatomic imaging devices are now becoming available, for example SPECT/CT or PET/CT. This review article describes the most important of the new components, part of which have already entered product development and part of which are still in the research phase. The review focus on the employment of modern semiconductor detectors in Nuclear Medicine. (orig.) [de

  15. Energy Storage Technology Development for Space Exploration

    Science.gov (United States)

    Mercer, Carolyn R.; Jankovsky, Amy L.; Reid, Concha M.; Miller, Thomas B.; Hoberecht, Mark A.

    2011-01-01

    The National Aeronautics and Space Administration is developing battery and fuel cell technology to meet the expected energy storage needs of human exploration systems. Improving battery performance and safety for human missions enhances a number of exploration systems, including un-tethered extravehicular activity suits and transportation systems including landers and rovers. Similarly, improved fuel cell and electrolyzer systems can reduce mass and increase the reliability of electrical power, oxygen, and water generation for crewed vehicles, depots and outposts. To achieve this, NASA is developing non-flow-through proton-exchange-membrane fuel cell stacks, and electrolyzers coupled with low permeability membranes for high pressure operation. The primary advantage of this technology set is the reduction of ancillary parts in the balance-of-plant fewer pumps, separators and related components should result in fewer failure modes and hence a higher probability of achieving very reliable operation, and reduced parasitic power losses enable smaller reactant tanks and therefore systems with lower mass and volume. Key accomplishments over the past year include the fabrication and testing of several robust, small-scale non-flow-through fuel cell stacks that have demonstrated proof-of-concept. NASA is also developing advanced lithium-ion battery cells, targeting cell-level safety and very high specific energy and energy density. Key accomplishments include the development of silicon composite anodes, lithiatedmixed- metal-oxide cathodes, low-flammability electrolytes, and cell-incorporated safety devices that promise to substantially improve battery performance while providing a high level of safety.

  16. Risk Management for Human Support Technology Development

    Science.gov (United States)

    jones, Harry

    2005-01-01

    NASA requires continuous risk management for all programs and projects. The risk management process identifies risks, analyzes their impact, prioritizes them, develops and carries out plans to mitigate or accept them, tracks risks and mitigation plans, and communicates and documents risk information. Project risk management is driven by the project goal and is performed by the entire team. Risk management begins early in the formulation phase with initial risk identification and development of a risk management plan and continues throughout the project life cycle. This paper describes the risk management approach that is suggested for use in NASA's Human Support Technology Development. The first step in risk management is to identify the detailed technical and programmatic risks specific to a project. Each individual risk should be described in detail. The identified risks are summarized in a complete risk list. Risk analysis provides estimates of the likelihood and the qualitative impact of a risk. The likelihood and impact of the risk are used to define its priority location in the risk matrix. The approaches for responding to risk are either to mitigate it by eliminating or reducing the effect or likelihood of a risk, to accept it with a documented rationale and contingency plan, or to research or monitor the risk, The Human Support Technology Development program includes many projects with independently achievable goals. Each project must do independent risk management, considering all its risks together and trading them against performance, budget, and schedule. Since the program can succeed even if some projects fail, the program risk has a complex dependence on the individual project risks.

  17. Development of a direct competitive enzyme-linked immunosorbent assay for parathion residue in food samples.

    Science.gov (United States)

    Gui, Wen-Jun; Liu, Yi-Hua; Wang, Chun-Mei; Liang, Xiao; Zhu, Guo-Nian

    2009-10-01

    A heterologous direct competitive enzyme-linked immunosorbent assay (ELISA) for parathion residue determination is described based on a monoclonal antibody and a new competitor. The effects of several physicochemical factors, such as methanol concentration, ionic strength, pH value, and sample matrix, on the performance of the ELISA were optimized for the sake of obtaining a satisfactory assay sensitivity. Results showed that when the assay medium was in the optimized condition (phosphate buffer solution [PBS] containing 10% [v/v] methanol and 0.2 mol/L NaCl at a pH value of 5.0), the sensitivity (estimated as the IC(50) value) and the limit of detection (LOD, estimated as the IC(10) value) were 1.19 and 0.08 ng/ml, respectively. The precision investigation indicated that the intraassay precision values all were below 10% and that the interassay precision values ranged from 4.89 to 19.12%. In addition, the developed ELISA showed a good linear correlation (r(2)=0.9962) to gas chromatography within the analyte's concentration range of 0.1 to 16 ng/ml. When applied to the fortified samples (parathion adding level: 5-15 microg/kg), the developed ELISA presented mean recoveries of 127.46, 122.52, 91.92, 124.01, 129.72, 99.37, and 87.17% for tomato, cucumber, banana, apple, orange, pear, and sugarcane, respectively. Results indicated that the established ELISA is a potential tool for parathion residue determination.

  18. Development of forage cryo minced fish technology

    Directory of Open Access Journals (Sweden)

    Titova S. A.

    2017-11-01

    Full Text Available The technology of grinding the frozen fish raw material using the useful model of a piston-type extrusion plant with cold working bodies for fodder minced fish (cryo minced fish feed by the method of cryoextrusion has been developed. The possibility of replacing the standard manufacturing operations (defrosting and subsequent grinding of raw materials to the one (cryoextrusion has been substantiated. The optimal shape of holes of the die for forcing the raw material has been determined in the form of "hourglass", the hole diameter is 7 mm, the length of the process of pushing is 40 s. It has been established that the finished product (cryo minced fish feed has a homogeneous grinding, juicy, crumbly texture, the temperature in the thickness of the product is equal to the temperature of the raw material before processing (–18 °C. The technological scheme of producing the minced fish feed by the method of cryoextrusion has been developed. Researching the chemical composition of fish and feed of mince produced by the traditional and developed methods it has been established that protein content in the cryo minced fish feed remains unchanged. The amino acid composition of cryo minced fish feed has been determined, the amount of essential amino acids is 365.6 mg/g of protein. In the proteins of the cryo minced fish feed methionine has been contained in the minimum quantity (10.0 mg/g protein and in the maximum – serine (155.3 mg/g protein. In the protein of the cryo minced fish feed the only limiting amino acid is methionine. The coefficient of rationality of the cryo minced fish feed whiting is equal to 0.33. The product is not toxic, is not subject to contamination with dangerous microorganisms, has high biological value, contains necessary in animal nutrition mineral elements, the energy value per 100 g of the product is 87 kcal. The combination of the processes of cryoextrusion and lyophilization allows to obtain a new food product with high

  19. Technology platforms: opportunities and development perspectives

    Science.gov (United States)

    Belyakov, G. P.; Avramchikova, N. T.; Belyakova, G. Y.; Chuvashova, M. N.

    2016-11-01

    The term “technology platform” is used to define thematic scopes in terms of which technological priorities are formed concerning state, business, science and education cooperation that are aimed to solve the problem of state technological independence. The authors have examined the implementation of this tool so that to concentrate essential resources in priority-driven vectors of science and technological advancement of a guiding cluster of innovative technologies in the field of space applications.

  20. Liquid metal reactor development. Development of LMR coolant technology

    Energy Technology Data Exchange (ETDEWEB)

    Nam, H. Y.; Choi, S. K.; Hwang, J. s.; Lee, Y. B.; Choi, B. H.; Kim, J. M.; Kim, Y. G.; Kim, M. J.; Lee, S. D.; Kang, Y. H.; Maeng, Y. Y.; Kim, T. R.; Park, J. H.; Park, S. J.; Cha, J. H.; Kim, D. H.; Oh, S. K.; Park, C. G.; Hong, S. H.; Lee, K. H.; Chun, M. H.; Moon, H. T.; Chang, S. H.; Lee, D. N.

    1997-07-15

    Following studies have been performed during last three years as the 1.2 phase study of the mid and long term nuclear technology development plan. First, the small scale experiments using the sodium have been performed such as the basic turbulent mixing experiment which is related to the design of a compact reactor, the flow reversal characteristics experiment by natural circulation which is necessary for the analysis of local flow reversal when the electromagnetic pump is installed, the feasibility test of the decay heat removal by wall cooling and the operation of electromagnetic pump. Second, the technology of operation mechanism of sodium facility is developed and the technical analysis and fundamental experiments of sodium measuring technology has been performed such as differential pressure measuring experiment, local flow rate measuring experimenter, sodium void fraction measuring experiment, under sodium facility, the free surface movement experiment and the side orifice pressure drop experiment. A new bounded convection scheme was introduced to the ELBO3D thermo-hydraulic computer code designed for analysis of experimental result. A three dimensional computer code was developed for the analysis of free surface movement and the analysis model of transmission of sodium void fraction was developed. Fourth, the small scale key components are developed. The submersible-in-pool type electromagnetic pump which can be used as primary pump in the liquid metal reactor is developed. The SASS which uses the Curie-point electromagnet and the mock-up of Pantograph type IVTM were manufactured and their feasibility was evaluated. Fifth, the high temperature characteristics experiment of stainless steel which is used as a major material for liquid metal reactor and the material characteristics experiment of magnet coil were performed. (author). 126 refs., 98 tabs., 296 figs.

  1. Innovations in the Assay of Un-Segregated Multi-Isotopic Grade TRU Waste Boxes with SuperHENC and FRAM Technology

    International Nuclear Information System (INIS)

    Simpson, A. P.; Barber, S.; Abdurrahman, N. M.

    2006-01-01

    The Super High Efficiency Neutron Coincidence Counter (SuperHENC) was originally developed by BIL Solutions Inc., Los Alamos National Laboratory (LANL) and Rocky Flats Environmental Technology Site (RFETS) for assay of transuranic (TRU) waste in Standard Waste Boxes (SWB) at Rocky Flats. This mobile system was a key component in the shipment of over 4,000 SWBs to the Waste Isolation Pilot Plant (WIPP) in Carlsbad, New Mexico. The system was WIPP certified in 2001 and operated at the site for four years. The success of this system, a passive neutron coincidence counter combined with high resolution gamma spectroscopy, led to the order of two new units, delivered to Hanford in 2004. Several new challenges were faced at Hanford: For example, the original RFETS system was calibrated for segregated waste streams such that metals, plastics, wet combustibles and dry combustibles were separated by 'Item Description Codes' prior to assay. Furthermore, the RFETS mission of handling only weapons grade plutonium, enabled the original SuperHENC to benefit from the use of known Pu isotopics. Operations at Hanford, as with most other DOE sites, generate un-segregated waste streams, with a wide diversity of Pu isotopics. Consequently, the new SuperHENCs are required to deal with new technical challenges. The neutron system's software and calibration methodology have been modified to encompass these new requirements. In addition, PC-FRAM software has been added to the gamma system, providing a robust isotopic measurement capability. Finally a new software package has been developed that integrates the neutron and gamma data to provide a final assay results and analysis report. The new system's performance has been rigorously tested and validated against WIPP quality requirements. These modifications, together with the mobile platform, make the new SuperHENC far more versatile in handling diverse waste streams and allow for rapid redeployment around the DOE complex. (authors)

  2. Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri.

    Science.gov (United States)

    Réveiller, Fabienne L; Cabanes, Pierre-André; Marciano-Cabral, Francine

    2002-05-01

    Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a fatal disease of the central nervous system that is acquired while swimming or diving in freshwater. A cDNA clone designated Mp2C15 obtained from N. fowleri was used as a probe to distinguish N. fowleri from other free-living amoebae. The Mp2C15 probe hybridized to genomic DNA from pathogenic N. fowleri and antigenically related non-pathogenic N. lovaniensis. Mp2C15 was digested with the restriction enzyme XbaI, resulting in two fragments, Mp2C15.G and Mp2C15.P. Four species of Naegleria and four species of Acanthamoeba were examined for reactivity with Mp2C15.P. Mp2C15.P was specific for N. fowleri and was used in the development of a nested PCR assay which is capable of detecting as little as 5 pg of N. fowleri DNA or five intact N. fowleri amoebae. In summary, a rapid, sensitive, and specific assay for the detection of N. fowleri was developed.

  3. Development of a PCR assay for identification of antibiotic resistance determinants at Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Cornelia CRĂCIUNAŞ

    2010-11-01

    Full Text Available The aim of the present study was to develop a PCR assay for identification of antibiotic resistance genes at Staphylococcus aureus clinical isolates. Thirty five isolates from the Emergency County Hospital and Clinical Hospital for Infectious Diseases in Cluj-Napoca, Romania, were analyzed. The bacteria were isolated over two years period, in 2007 and 2009 and serotyped at the Clinical Hospital of Infectious Disease, Cluj-Napoca. In order to develop a PCR assay for identification of antibiotic resistance genes at S. aureus clinical isolates, the bacterial strains were tested for the presence of the tst, sea, mecA, femA, ermC and ermA genes using the polymerase chain reaction and gene-specific primers. We succeeded in amplifying mecA gene to all isolates that exhibit methicillin resistance. Our result concord 100% with standard culture method but they were obtained in 5 hours instead of four days. In order to increase the efficiency of PCR typing and reduce reagent costs, multiple sets of primers were included in a single reaction tube in a multiplex PCR. We succeeded in elaborating a multiplex PCR for mecA and femA genes.

  4. Strategic decision-making for technology implementation in developing countries

    OpenAIRE

    Hipkin, Ian; Bennett, David

    2003-01-01

    Researchers and managers stress the importance of long-term technology strategies to develop technological capabilities for global competitive advantage. This paper explores the relationship between technology decision-making and strategy in technology transfer (TT) in developing countries, with special reference to South Africa. Earlier research by the authors considered technology and operations integration in developing countries and identified factors that were important to managers in th...

  5. Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos

    Directory of Open Access Journals (Sweden)

    Lee Okhyun

    2012-06-01

    Full Text Available Abstract Background Oestrogenic contaminants are widespread in the aquatic environment and have been shown to induce adverse effects in both wildlife (most notably in fish and humans, raising international concern. Available detecting and testing systems are limited in their capacity to elucidate oestrogen signalling pathways and physiological impacts. Here we developed a transient expression assay to investigate the effects of oestrogenic chemicals in fish early life stages and to identify target organs for oestrogenic effects. To enhance the response sensitivity to oestrogen, we adopted the use of multiple tandem oestrogen responsive elements (EREc38 in a Tol2 transposon mediated Gal4ff-UAS system. The plasmid constructed (pTol2_ERE-TATA-Gal4ff, contains three copies of oestrogen response elements (3ERE that on exposure to oestrogen induces expression of Gal4ff which this in turn binds Gal4-responsive Upstream Activated Sequence (UAS elements, driving the expression of a second reporter gene, EGFP (Enhanced Green Fluorescent Protein. Results The response of our construct to oestrogen exposure in zebrafish embryos was examined using a transient expression assay. The two plasmids were injected into 1–2 cell staged zebrafish embryos, and the embryos were exposed to various oestrogens including the natural steroid oestrogen 17ß-oestradiol (E2, the synthetic oestrogen 17α- ethinyloestradiol (EE2, and the relatively weak environmental oestrogen nonylphenol (NP, and GFP expression was examined in the subsequent embryos using fluorescent microscopy. There was no GFP expression detected in unexposed embryos, but specific and mosaic expression of GFP was detected in the liver, heart, somite muscle and some other tissue cells for exposures to steroid oestrogen treatments (EE2; 10 ng/L, E2; 100 ng/L, after 72 h exposures. For the NP exposures, GFP expression was observed at 10 μg NP/L after 72 h (100 μg NP/L was toxic to the fish. We

  6. Development of IT-based data communication network technology

    International Nuclear Information System (INIS)

    Hong, Seok Boong; Jeong, K. I.; Yoo, Y. R.

    2010-10-01

    - Developing broadband high-reliability real-time communications technology for NPP - Developing reliability and performance validation technology for communications network - Developing security technology for NPP communications network - Developing field communications network for harsh environment of NPP - International standard registration(Oct. 28, 2009, IEC 61500

  7. User Interface Technology for Formal Specification Development

    Science.gov (United States)

    Lowry, Michael; Philpot, Andrew; Pressburger, Thomas; Underwood, Ian; Lum, Henry, Jr. (Technical Monitor)

    1994-01-01

    Formal specification development and modification are an essential component of the knowledge-based software life cycle. User interface technology is needed to empower end-users to create their own formal specifications. This paper describes the advanced user interface for AMPHION1 a knowledge-based software engineering system that targets scientific subroutine libraries. AMPHION is a generic, domain-independent architecture that is specialized to an application domain through a declarative domain theory. Formal specification development and reuse is made accessible to end-users through an intuitive graphical interface that provides semantic guidance in creating diagrams denoting formal specifications in an application domain. The diagrams also serve to document the specifications. Automatic deductive program synthesis ensures that end-user specifications are correctly implemented. The tables that drive AMPHION's user interface are automatically compiled from a domain theory; portions of the interface can be customized by the end-user. The user interface facilitates formal specification development by hiding syntactic details, such as logical notation. It also turns some of the barriers for end-user specification development associated with strongly typed formal languages into active sources of guidance, without restricting advanced users. The interface is especially suited for specification modification. AMPHION has been applied to the domain of solar system kinematics through the development of a declarative domain theory. Testing over six months with planetary scientists indicates that AMPHION's interactive specification acquisition paradigm enables users to develop, modify, and reuse specifications at least an order of magnitude more rapidly than manual program development.

  8. Development and Evaluation of Three Real-Time PCR Assays for Genotyping and Source Tracking Cryptosporidium spp. in Water

    Science.gov (United States)

    Li, Na; Neumann, Norman F.; Ruecker, Norma; Alderisio, Kerri A.; Sturbaum, Gregory D.; Villegas, Eric N.; Chalmers, Rachel; Monis, Paul; Feng, Yaoyu

    2015-01-01

    The occurrence of Cryptosporidium oocysts in drinking source water can present a serious public health risk. To rapidly and effectively assess the source and human-infective potential of Cryptosporidium oocysts in water, sensitive detection and correct identification of oocysts to the species level (genotyping) are essential. In this study, we developed three real-time PCR genotyping assays, two targeting the small-subunit (SSU) rRNA gene (18S-LC1 and 18S-LC2 assays) and one targeting the 90-kDa heat shock protein (hsp90) gene (hsp90 assay), and evaluated the sensitivity and Cryptosporidium species detection range of these assays. Using fluorescence resonance energy transfer probes and melt curve analysis, the 18S-LC1 and hsp90 assays could differentiate common human-pathogenic species (C. parvum, C. hominis, and C. meleagridis), while the 18S-LC2 assay was able to differentiate nonpathogenic species (such as C. andersoni) from human-pathogenic ones commonly found in source water. In sensitivity evaluations, the 18S-LC2 and hsp90 genotyping assays could detect as few as 1 Cryptosporidium oocyst per sample. Thus, the 18S-LC2 and hsp90 genotyping assays might be used in environmental monitoring, whereas the 18S-LC1 genotyping assay could be useful for genotyping Cryptosporidium spp. in clinical specimens or wastewater samples. PMID:26092455

  9. Development of a dot blot assay using gene probes for the detection of enteroviruses in water

    International Nuclear Information System (INIS)

    Margolin, A.B.

    1986-01-01

    Enteric viruses are viruses which replicate in the intestinal tract of man and animals. One mode of transmission for enteric viruses is the fecal-oral route. Drinking water which has been contaminated with sewage or sewage effluent has been implicated as a means for the spread of enteric viruses. Current methods for the detection of enteric viruses in water requires the use of animal cell culture. This technique has several drawbacks. More rapid techniques, such as fluorescent antibody or radioimmunoassay do not have the needed sensitivity to detect the low levels of virus found in contaminated water. An alternative technique for the detection of viruses in water was sought. Recent advances in recombinant DNA technology now makes it possible to detect viruses without the use of cell culture or antibodies. Gene probes that hybridize to the RNA of poliovirus and hepatitis A virus were tested for their ability to detect different enteric viruses. The probes were labeled with 32 P dCTP and 32 P dATP to a specific activity greater then 1.0 x 10 9 cpm/ug DNA. One infectious unit of poliovirus and hepatitis A virus was detected using labeled cDNA probes. Upon comparison, the dot blot assay was as sensitive as tissue culture for the detection of poliovirus in beef extract, secondary effluent, and tap water. Environmental samples, such as secondary effluent, reclaimed wastewater and unchlorinated drinking water were also assayed for poliovirus and hepatitis A virus with the use of gene probes. The results presented here offer an alternative method for screening water samples for the presence of enteric viruses

  10. Development of conformal respirator monitoring technology

    International Nuclear Information System (INIS)

    Shonka, J.J.; Weismann, J.J.; Logan, R.J.

    1997-04-01

    This report summarizes the results of a Small Business Innovative Research Phase II project to develop a modular, surface conforming respirator monitor to improve upon the manual survey techniques presently used by the nuclear industry. Research was performed with plastic scintillator and gas proportional modules in an effort to find the most conducive geometry for a surface conformal, position sensitive monitor. The respirator monitor prototype developed is a computer controlled, position-sensitive detection system employing 56 modular proportional counters mounted in molds conforming to the inner and outer surfaces of a commonly used respirator (Scott Model 801450-40). The molds are housed in separate enclosures and hinged to create a open-quotes waffle-ironclose quotes effect so that the closed monitor will simultaneously survey both surfaces of the respirator. The proportional counter prototype was also designed to incorporate Shonka Research Associates previously developed charge-division electronics. This research provided valuable experience into pixellated position sensitive detection systems. The technology developed can be adapted to other monitoring applications where there is a need for deployment of many traditional radiation detectors

  11. Genetic mouse embryo assay: improving performance and quality testing for assisted reproductive technology (ART) with a functional bioassay.

    Science.gov (United States)

    Gilbert, Rebecca S; Nunez, Brandy; Sakurai, Kumi; Fielder, Thomas; Ni, Hsiao-Tzu

    2016-03-24

    Growing concerns about safety of ART on human gametes, embryos, clinical outcomes and long-term health of offspring require improved methods of risk assessment to provide functionally relevant assays for quality control testing and pre-clinical studies prior to clinical implementation. The one-cell mouse embryo assay (MEA) is the most widely used for development and quality testing of human ART products; however, concerns exist due to the insensitivity/variability of this bioassay which lacks standardization and involves subjective analysis by morphology alone rather than functional analysis of the developing embryos. We hypothesized that improvements to MEA by the use of functional molecular biomarkers could enhance sensitivity and improve detection of suboptimal materials/conditions. Fresh one-cell transgenic mouse embryos with green fluorescent protein (GFP) expression driven by Pou6f1 or Cdx2 control elements were harvested and cultured to blastocysts in varied test and control conditions to compare assessment by standard morphology alone versus the added dynamic expression of GFP for screening and selection of critical raw materials and detection of suboptimal culture conditions. Transgenic mouse embryos expressing functionally relevant biomarkers of normal early embryo development can be used to monitor the developmental impact of culture conditions. This novel approach provides a superior MEA that is more meaningful and sensitive for detection of embryotoxicity than morphological assessment alone.

  12. Development of Industrial Process Diagnosis and Measurement Technology

    International Nuclear Information System (INIS)

    Jung, Sung Hee; Kim, Jong Bum; Moon, Jin Ho

    2010-04-01

    Section 1. Industrial Gamma CT Technology for Process Diagnosis: The project is aimed to develop industrial process gamma tomography system for investigation on structural and physical malfunctioning and process media distribution by means of sealed gamma source and radioactive materials. Section 2. Development of RI Hydraulic Detection Technology for Industrial Application: The objectives in this study are to develop the evaluation technology of the hydrological characteristics and the hydraulic detection technology using radioisotope, and to analyze the hydrodynamics and pollutant transport in water environment like surface and subsurface. Section 3. Development of RT-PAT System for Powder Process Diagnosis: The objective of this project is the development of a new radiation technology to improve the accuracy of the determination of moisture content in a powder sample by using radiation source through the so-called RT-PAT (Radiation Technology-Process Analytical Technology), which is a new concept of converging technology between the radiation technology and the process analytical technology

  13. Development of industrial process diagnosis and measurement technology

    International Nuclear Information System (INIS)

    Jung, Sunghee; Kim, Jongbum; Moon, Jinho; Suh, Kyungsuk; Kim, Jongyun

    2012-04-01

    Section1. Industrial Gamma CT Technology for Process Diagnosis The project is aimed to develop industrial process gamma tomography system for investigation on structural and physical malfunctioning and process media distribution by means of sealed gamma source and radioactive materials. Section2. Development of RI Hydraulic Detection Technology for Industrial Application The objectives in this study are to develop the evaluation technology of the hydrological characteristics and the hydraulic detection technology using radioisotope, and to analyze the hydrodynamics and pollutant transport in water environment like surface and subsurface. Section3. Development of RT-PAT System for Powder Process Diagnosis The objective of this project is the development of a new radiation technology to improve the accuracy of the determination of moisture content in a powder sample by using radiation source through the so-called RT-PAT (Radiation Technology-Process Analytical Technology), which is a new concept of converging technology between the radiation technology and the process analytical technology

  14. Protection of civil rights and technological development

    International Nuclear Information System (INIS)

    Wagner, H.

    1985-01-01

    The question of how the consequences of technological development are to be mastered on the legal level is not primarily a problem of the administrative procedure. The essential point in the discussion on protection of basic rights is not to be seen in the citizen's right to participate in administrative procedures, but rather in the substantive civil rights of individual persons as against the government, together with the system of values established in the Basic Law and derived from the basic rights, as well as in the proper functioning of the principle of separation of powers (legislature, administration, and judicature). The law on procedure and thus the idea of (protection of civil rights by rules of procedure) in this context only have an enhancing effect. Practical protection of civil rights is also ascertained in the Federal Republic of Germany by the particularly well-established guarantee of access to the courts. Another idea to be mentioned in this context is an approach that can be termed as 'protection of civil rights by government-funded research'. Other facts contributing to building a protective shield against infringement of civil rights by technology, activities or installations, include e.g. the federal structure of the state and the administration, type and scope of organisation of the public service, the established system of environmental protection and the safety provided by the current system of expert opinions. (orig./HSCH) [de

  15. Development of fission Mo-99 production technology

    International Nuclear Information System (INIS)

    Park, Jin Ho; Choung, W. M.; Lee, K. I. and others

    2001-05-01

    This R and D project is planed to supply domestic demands of Mo-99 through fission route, and consequently this project will be expected to rise up utilization of HANARO and KAERI's capability for marketing extension into domestic and oversea radiopharmaceutical market. HEU and LEU target types are decided and designed for fission Mo-99 production in domestic. Experimental study of target fabrication technology was performed and developed processing equipments. And conceptual design of target loading/unloading in/from HANARO device are performed. Tracer test of Mo-99 separation and purification process was performed, test results reach to Mo-99 recovery yield above 80% and decontamination factor above 1600. Combined Mo-99 separation and purification process was decided for hot test scheduled from next year, and performance test was performed. Conceptual design for modification of existing hot cell for fission Mo-99 production facility was performed and will be used for detail design. Assumption for the comparison of LEU and HEU target in fission Mo-99 production process were suggested and compared of merits and demerits in view of fabrication technology and economy feasibility

  16. Development of fission Mo-99 production technology

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jin Ho; Choung, W. M.; Lee, K. I. and others

    2001-05-01

    This R and D project is planed to supply domestic demands of Mo-99 through fission route, and consequently this project will be expected to rise up utilization of HANARO and KAERI's capability for marketing extension into domestic and oversea radiopharmaceutical market. HEU and LEU target types are decided and designed for fission Mo-99 production in domestic. Experimental study of target fabrication technology was performed and developed processing equipments. And conceptual design of target loading/unloading in/from HANARO device are performed. Tracer test of Mo-99 separation and purification process was performed, test results reach to Mo-99 recovery yield above 80% and decontamination factor above 1600. Combined Mo-99 separation and purification process was decided for hot test scheduled from next year, and performance test was performed. Conceptual design for modification of existing hot cell for fission Mo-99 production facility was performed and will be used for detail design. Assumption for the comparison of LEU and HEU target in fission Mo-99 production process were suggested and compared of merits and demerits in view of fabrication technology and economy feasibility.

  17. Development of Atmospheric Kr-85 Analysis Technology

    Energy Technology Data Exchange (ETDEWEB)

    Keum, Dong Kwon; Lee, Han Soo; Choi, Hei Hu; Kang, Hee Seok; Lee, Wan Ro; Lee, Chang Woo

    2004-12-15

    In order to establish effectively the atmospheric Kr-85 analysis system. the preliminary study, including the analysis of state of the art, the construction of a Kr collection system and its performance test, and the set up of future plan have been performed. As the result of the performance test of the collection system. the concentration of Kr in air was concentrated by about 700 times, compared to that before the collection, and the collected Kr gas volume was about 2.1 cc, which was about 10 times larger than the minimum Kr gas volume (about 0.2cc) required for the measurement of the radioactivity using GM tube. Three-stage action plan for the establishment of the Kr-85 analysis system is presented. In the next stage, the focused works include the completion of the design technology for the Kr collection system via a practical application and the development of critical unit technology for the separation and purification of sample.

  18. Implementation and development of an automated, ultra-high-capacity, acoustic, flexible dispensing platform for assay-ready plate delivery.

    Science.gov (United States)

    Griffith, Dylan; Northwood, Roger; Owen, Paul; Simkiss, Ellen; Brierley, Andrew; Cross, Kevin; Slaney, Andrew; Davis, Miranda; Bath, Colin

    2012-10-01

    Compound management faces the daily challenge of providing high-quality samples to drug discovery. The advent of new screening technologies has seen demand for liquid samples move toward nanoliter ranges, dispensed by contactless acoustic droplet ejection. Within AstraZeneca, a totally integrated assay-ready plate production platform has been created to fully exploit the advantages of this technology. This enables compound management to efficiently deliver large throughputs demanded by high-throughput screening while maintaining regular delivery of smaller numbers of compounds in varying plate formats for cellular or biochemical concentration-response curves in support of hit and lead optimization (structure-activity relationship screening). The automation solution, CODA, has the capability to deliver compounds on demand for single- and multiple-concentration ranges, in batch sizes ranging from 1 sample to 2 million samples, integrating seamlessly into local compound and test management systems. The software handles compound orders intelligently, grouping test requests together dependent on output plate type and serial dilution ranges so that source compound vessels are shared among numerous tests, ensuring conservation of sample, reduced labware and costs, and efficiency of work cell logistics. We describe the development of CODA to address the customer demand, challenges experienced, learning made, and subsequent enhancements.

  19. Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus).

    Science.gov (United States)

    Paudel, Sarad; Villanueva, Marvin A; Mikota, Susan K; Nakajima, Chie; Gairhe, Kamal P; Subedi, Suraj; Rayamajhi, Nabin; Sashika, Mariko; Shimozuru, Michito; Matsuba, Takashi; Suzuki, Yasuhiko; Tsubota, Toshio

    2016-08-01

    We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.

  20. Safeguards technology research and development at CIAE

    International Nuclear Information System (INIS)

    Yang Qun

    2001-01-01

    Full text: China Institute of Atomic Energy (CIAE) is a multi-disciplinary institute under the leadership of China National Nuclear Corporation (CNNC). The Laboratory of Technical Research for Nuclear Safeguards was established at CIAE in 1991 to develop safeguards technology and to provide technical assistance to competent authorities for nuclear material management and control, which became one of the key laboratories approved by CNNC in 1993. The main research works for safeguards at CIAE include: nuclear material control and accounting, facilities license review and assessment, domestic inspection, NDA and DA analysis, physical protection and technical training. Research and development of equipment and technique for safeguards has been continuing at CIAE. A variety of NDA equipment that has different resolution and analysis capability has been developed. Method of NDA measurement has been investigated for nuclear material with different characteristics. Mathematics method such as Monte Carlo simulation is applied in NDA. Advanced destructive analysis (DA) instrument is installed at laboratory of CIAE, such as TIMS, ICP-MS and electronic chemistry analyzing system. The high accuracy results of element analysis and isotopic analysis for nuclear material can be obtained. It is possible to measure the types and quantities of nuclear material in a given area by means of NDA and DA. Physical protection system has also been developed. It consists of access control and management, various alarm (including perimeter alarm, intrusion alarms, fire alarms), video and audio monitors, intercommunication set and central console. The system can meet technical requirement for safeguards of first rank. Nuclear material accounting is an important aspect of safeguards research at CIAE. The computer software related to material accounting has been developed. It is the important task for scientists at CIAE to design and review nuclear accounting systems in various facilities. For

  1. Development of proliferation resistant isotope separation technology

    International Nuclear Information System (INIS)

    Jeong, Doyoung; Ko, Kwanghoon; Kim, Taeksoo; Park, Hyunmin; Lim, Gwon; Cha, Yongho; Han, Jaemin; Baik, Sunghoon; Cha, Hyungki

    2012-02-01

    This project was accomplished with an aim of establishing the industrial facilities for isotope separation in Korea. The experiment for the measurement of neutrino mass that has been an issue in physics, needs very much of enriched calcium-48 isotope. However, calcium-48 isotope can be produced only by the electro-magnetic method and, thus, its price is very expensive. Therefore, we expect that ALSIS can replace the electro-magnetic method for calcium-48 isotope production. In this research stage, the research was advanced systematically with core technologies, such as atomic vapor production, the measurement of vapor characteristics and stable and powerful laser development. These researches will be the basis of the next research stages. In addition, the international research trends and cooperation results are reported in this report

  2. EPA-developed, patented technologies available for licensing

    Science.gov (United States)

    Under the Federal Technology Transfer Act (FTTA), Federal Agencies can patent inventions developed during the course of research. These technologies can then be licensed to businesses or individuals for further development and sale in the marketplace.

  3. Risk analysis and technology assessment in support of technology development

    NARCIS (Netherlands)

    Wezel, van Annemarie P.; Lente, van Harro; Sandt, van de Johannes J.M.; Bouwmeester, Hans; Vandeberg, Rens L.J.; Sips, Adrienne J.A.M.

    2018-01-01

    Governments invest in “key enabling technologies,” such as nanotechnology, to solve societal challenges and boost the economy. At the same time, governmental agencies demand risk reduction to prohibit any often unknown adverse effects, and industrial parties demand smart approaches to reduce

  4. Development of Integrated Information Technology System

    National Research Council Canada - National Science Library

    Gilstrap, Scott

    2004-01-01

    The Integrated Medical Information Technology System (IMITS) Program is focused on implementation of advanced technology solutions that eliminate inefficiencies, increase utilization and improve quality of care for active duty forces...

  5. Development of the Integrated Information Technology System

    National Research Council Canada - National Science Library

    2005-01-01

    The Integrated Medical Information Technology System (IMITS) Program is focused on implementation of advanced technology solutions that eliminate inefficiencies, increase utilization and improve quality of care for active duty forces...

  6. Development of a Practical Complete-Kill Assay to Evaluate Anti-Acanthamoeba Drugs

    Science.gov (United States)

    Kowalski, Regis P.; Abdel Aziz, Salwa; Romanowski, Eric G.; Shanks, RobertM. Q.; Nau, Amy C.; Raju, Leela V.

    2015-01-01

    IMPORTANCE Acanthamoeba keratitis is a debilitating eye disease that requires effective topical drugtherapy. Currently, there is no standard in vitro test to evaluate anti-Acanthamoeba drugs. OBJECTIVE To develop a practical in vitro complete-kill assay to assess anti-Acanthamoeba drugs. DESIGN AND SETTING Isolates of Acanthamoeba strains (n = 15) evaluated in a clinical laboratory. An in vitro laboratory assay was created to determine whether polyhexamethylene biguanide, 0.02%, chlorhexidine digluconate, 0.02%, hexamidine diisethioonate, 0.1%, and voriconazole, 1.0%, were effective in completely killing 15 different isolates of Acanthamoeba at time points of 24, 48, and 72 hours in comparison with a saline control. Each 0.5-mL volume of drug was inoculated with 0.1 mL of Acanthamoeba cysts (range, 1–3×106/mL) (determined with a hemacytometer) and allowed to incubate at 30°C. At the time points listed, 0.05 mL from each treatment group was inoculated onto nonnutrient agar overlaid with Enterobacter aerogenes. The plates were microscopically examined for growth 1 and 2 weeks after inoculation. At 2 weeks, all plates were subcultured onto a fresh medium. At another 7 days, the growth in subculture at each time point was graded "1" for growth and "0" for no growth. MAIN OUTCOMES AND MEASURES The cumulative grades of 3 time points (range, 0–3) for each drug and isolate were nonparametrically compared to determine differences in growth between the drugs. The "kill" incidence rates over the 3 time points were also compared. RESULTS In vitro testing determined that antiacanthamoebal efficacy (determined by the median growth grade and the kill incidence rate) was more prominent for hexamidine diisethioonate (median growth grade, 0.0; kill incidence rate, 93% [14 of 15 isolates]) and polyhexamethylene biguanide (median growth grade, 0.0; kill incidence rate, 80% [12 of 15 isolates]) than for chlorhexidine digluconate (median growth grade, 1.0; kill incidence rate, 40

  7. Interactions of energy technology development and new energy exploitation with water technology development in China

    International Nuclear Information System (INIS)

    Liang, Sai; Zhang, Tianzhu

    2011-01-01

    Interactions of energy policies with water technology development in China are investigated using a hybrid input-output model and scenario analysis. The implementation of energy policies and water technology development can produce co-benefits for each other. Water saving potential of energy technology development is much larger than that of new energy exploitation. From the viewpoint of proportions of water saving co-benefits of energy policies, energy sectors benefit the most. From the viewpoint of proportions of energy saving and CO 2 mitigation co-benefits of water technology development, water sector benefits the most. Moreover, economic sectors are classified into four categories concerning co-benefits on water saving, energy saving and CO 2 mitigation. Sectors in categories 1 and 2 have big direct co-benefits. Thus, they can take additional responsibility for water and energy saving and CO 2 mitigation. If China implements life cycle materials management, sectors in category 3 can also take additional responsibility for water and energy saving and CO 2 mitigation. Sectors in category 4 have few co-benefits from both direct and accumulative perspectives. Thus, putting additional responsibility on sectors in category 4 might produce pressure for their economic development. -- Highlights: ► Energy policies and water technology development can produce co-benefits for each other. ► For proportions of water saving co-benefits of energy policies, energy sectors benefit the most. ► For proportions of energy saving and CO 2 mitigation co-benefits of water policy, water sector benefits the most. ► China’s economic sectors are classified into four categories for policy implementation at sector scale.

  8. Development and validation of a Luminex assay for detection of a predictive biomarker for PROSTVAC-VF therapy.

    Directory of Open Access Journals (Sweden)

    Julie L Lucas

    Full Text Available Cancer therapies can provide substantially improved survival in some patients while other seemingly similar patients receive little or no benefit. Strategies to identify patients likely to respond well to a given therapy could significantly improve health care outcomes by maximizing clinical benefits while reducing toxicities and adverse effects. Using a glycan microarray assay, we recently reported that pretreatment serum levels of IgM specific to blood group A trisaccharide (BG-Atri correlate positively with overall survival of cancer patients on PROSTVAC-VF therapy. The results suggested anti-BG-Atri IgM measured prior to treatment could serve as a biomarker for identifying patients likely to benefit from PROSTVAC-VF. For continued development and clinical application of serum IgM specific to BG-Atri as a predictive biomarker, a clinical assay was needed. In this study, we developed and validated a Luminex-based clinical assay for measuring serum IgM specific to BG-Atri. IgM levels were measured with the Luminex assay and compared to levels measured using the microarray for 126 healthy individuals and 77 prostate cancer patients. This assay provided reproducible and consistent results with low %CVs, and tolerance ranges were established for the assay. IgM levels measured using the Luminex assay were found to be highly correlated to the microarray results with R values of 0.93-0.95. This assay is a Laboratory Developed Test (LDT and is suitable for evaluating thousands of serum samples in CLIA certified laboratories that have validated the assay. In addition, the study demonstrates that discoveries made using neoglycoprotein-based microarrays can be readily migrated to a clinical assay.

  9. Development and validation of a Luminex assay for detection of a predictive biomarker for PROSTVAC-VF therapy

    Science.gov (United States)

    Lucas, Julie L.; Tacheny, Erin A.; Ferris, Allison; Galusha, Michelle; Srivastava, Apurva K.; Ganguly, Aniruddha; Williams, P. Mickey; Sachs, Michael C.; Thurin, Magdalena; Tricoli, James V.; Ricker, Winnie; Gildersleeve, Jeffrey C.

    2017-01-01

    Cancer therapies can provide substantially improved survival in some patients while other seemingly similar patients receive little or no benefit. Strategies to identify patients likely to respond well to a given therapy could significantly improve health care outcomes by maximizing clinical benefits while reducing toxicities and adverse effects. Using a glycan microarray assay, we recently reported that pretreatment serum levels of IgM specific to blood group A trisaccharide (BG-Atri) correlate positively with overall survival of cancer patients on PROSTVAC-VF therapy. The results suggested anti-BG-Atri IgM measured prior to treatment could serve as a biomarker for identifying patients likely to benefit from PROSTVAC-VF. For continued development and clinical application of serum IgM specific to BG-Atri as a predictive biomarker, a clinical assay was needed. In this study, we developed and validated a Luminex-based clinical assay for measuring serum IgM specific to BG-Atri. IgM levels were measured with the Luminex assay and compared to levels measured using the microarray for 126 healthy individuals and 77 prostate cancer patients. This assay provided reproducible and consistent results with low %CVs, and tolerance ranges were established for the assay. IgM levels measured using the Luminex assay were found to be highly correlated to the microarray results with R values of 0.93–0.95. This assay is a Laboratory Developed Test (LDT) and is suitable for evaluating thousands of serum samples in CLIA certified laboratories that have validated the assay. In addition, the study demonstrates that discoveries made using neoglycoprotein-based microarrays can be readily migrated to a clinical assay. PMID:28771597

  10. Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus.

    Science.gov (United States)

    Garg, Himanshu; Sedano, Melina; Plata, Gabrielle; Punke, Erin B; Joshi, Anjali

    2017-10-15

    Recent worldwide outbreaks of Zika virus (ZIKV) infection and the lack of an approved vaccine raise serious concerns regarding preparedness to combat this emerging virus. We used a virus-like particle (VLP)-based approach to develop a vaccine and a microneutralization assay for ZIKV. A synthetic capsid-premembrane-envelope (C-prM-E) gene construct of ZIKV was used to generate reporter virus particles (RVPs) that package a green fluorescent protein (GFP) reporter-expressing West Nile virus (WNV) replicon. The assay was adapted to a 96-well format, similar to the plaque reduction neutralization test (PRNT), and showed high reproducibility with specific detection of ZIKV neutralizing antibodies. Furthermore, C-prM-E and prM-E VLPs were tested as vaccine candidates in mice and compared to DNA vaccination. While the ZIKV prM-E construct alone was sufficient for generating VLPs, efficient VLP production from the C-prM-E construct could be achieved in the presence of the WNV NS2B-3 protease, which cleaves C from prM, allowing virus release. Immunization studies in mice showed that VLPs generated higher neutralizing antibody titers than those with the DNA vaccines, with C-prM-E VLPs giving slightly higher titers than those with prM-E VLPs. The superiority of C-prM-E VLPs suggests that inclusion of capsid may have benefits for ZIKV and other flaviviral VLP vaccines. To facilitate the VLP platform, we generated a stable cell line expressing high levels of ZIKV prM-E proteins that constitutively produce VLPs as well as a cell line expressing ZIKV C-prM-E proteins for RVP production. While several vaccine platforms have been proposed for ZIKV, this study describes a safe, effective, and economical VLP-based vaccine against ZIKV. IMPORTANCE To address the growing Zika virus epidemic, we undertook this study with two objectives: first, to develop a safe, effective, and economical vaccine for ZIKV, and second, to develop a rapid and versatile assay to detect the anti-ZIKV immune

  11. Development of Coated Particle Fuel Technology

    International Nuclear Information System (INIS)

    Cho, Moon Sung; Kim, B. G.; Kim, Y. K.

    2009-04-01

    UO 2 kernel fabrication technology was developed at the lab sacle(20∼30g-UO 2 /batch). The GSP technique, modified method of sol-gel process, was used in the preparation of spherical ADU gel particle and these particles were converted to UO 3 and UO 2 phases in calcination furnace and sintering furnace respectively. Based on the process variables optimized using simulant kernels in 1-2 inch beds, SiC TRISO-coated particles were fabricated using UO 2 kernel. Power densities of TRISO coated particle fuels and gamma heat of the tubes are calculated as functions of vertical location of the fuel specimen in the irradiation holes by using core physics codes, MCNP and Helios. A finite model was developed for the calculations of temperatures and stresses of the specimen and the irradiation tubes. Dimensions of the test tubes are determined based on the temperatures and stresses as well as the gamma heat generated at the given condition. 9 modules of the COPA code (MECH, FAIL, TEMTR, TEMBL, TEMPEB, FPREL, MPRO, BURN, ABAQ), the MECH, FAIL, TEMTR, TEMBL, TEMPEB, and FPREL were developed. The COPA-FPREL was verified through IAEA CRP-6 accident benchmarking problems. KAERI participated in the round robin test of IAEA CRP-6 program to characterize the diameter, sphericity, coating thickness, density and anisotropy of coated particles provided by Korea, USA and South Africa. The inspection and test plan describing specifications and inspection method of coated particles was developed to confirm the quality standard of coated particles. The quality inspection instructions were developed for the inspection of coated particles by particle size analyzer, density inspection of coating layers by density gradient column, coating thickness inspection by X-ray, and inspection of optical anistropy factor of PyC layer. The quality control system for the TRISO-coated particle fuel was derived based on the status of quality control systems of other countries

  12. Development of a whole-cell-based screening method for a carotenoid assay using aerial microalgae.

    Science.gov (United States)

    Aburai, Nobuhiro; Kazama, Hiroaki; Tsuruoka, Atsushi; Goto, Mizuki; Abe, Katsuya

    2018-02-20

    Non-destructive approaches based on the application of optical spectroscopy are important for monitoring carotenoid accumulation in a whole cell cultured under various conditions. A simple and rapid assay utilizing aerial microalgae helps to identify stress conditions that can efficiently enhance the carotenogenesis in photosynthetic organisms. The spectra of cell suspensions were characterized in the aerial microalga Coelastrella sp. KGU-Y002, which are unicellular and undifferentiated. Total carotenoid contents could be successfully estimated on the basis of the absorbance values of the cell suspensions and calibration data analyzed by HPLC (high-performance liquid chromatography). A novel screening method, the so-called "whole-cell-based screening method" for carotenoid assays (WCA), was developed based on this procedure. It was possible to investigate the effects of various stresses on carotenoid accumulation in the aerial microalga by adapting this bioassay to a 96-well microtiter plate. When bioactive compounds were screened from our library of plant extracts using this method, an active compound was identified from the plant extract. Copyright © 2018 Elsevier B.V. All rights reserved.

  13. Development of a radioreceptor assay for {beta}{sub 2} adrenergic agonists

    Energy Technology Data Exchange (ETDEWEB)

    Helbo, V. [Lab. d`analyse des denrees alimentaires d`origine animale, Faculte de Medecine Veterinaire de l`Universite, Liege (Belgium); Vandenbroeck, M. [Lab. d`analyse des denrees alimentaires d`origine animale, Faculte de Medecine Veterinaire de l`Universite, Liege (Belgium); Maghuin-Rogister, G. [Lab. d`analyse des denrees alimentaires d`origine animale, Faculte de Medecine Veterinaire de l`Universite, Liege (Belgium)

    1994-05-01

    Several {beta}{sub 2} adrenergic agonists are illegally used as growth promoters in meat production. We have developed and evaluated a radioreceptor assay for the multianalyte detection of these compounds. The method is based on a competition for binding with receptors (plasma membranes prepared from bovine teat muscles) between a radioactive tracer ({sup 3}H-dihydroalprenolol) and {beta}{sub 2} agonist residues present in the samples. The method has been validated for three {beta}{sub 2} agonists (clenbuterol, mabuterol and cimaterol) in bovine urine samples. The detection limit (mean of ``blank`` values + 3 SEM) in urine was 2.4 ppb clenbuterol. Using this procedure, samples containing at least 5 ppb of clenbuterol, mabuterol or cimaterol could be identified as positive for the presence of {beta}{sub 2} agonists. (orig.) [Deutsch] Mehrere {beta}{sub 2} adrenerge Agonisten werden illegal als Wachstumsfoerderer in der Fleischproduktion eingesetzt. Wir entwickelten und testeten einen RRA (``Radioreceptor Assay``) zur Mehrfachrueckstandsanalyse dieser Zusammensetzungen. Die Methode basiert auf einer Kompetition eines radioaktiven Markers ({sup 3}H-dihydroalpenolol) mit den Rueckstaenden der {beta}{sub 2} Agonisten der Proben um Bindungsstellen der Rezeptoren (Plasmamembranen, welche aus Muskelzellen von Rinderzitzen gewonnen wurden). Die Methode wurde fuer 3 {beta}{sub 2} Agonisten (Clenbuterol, Mabuterol und Cimaterol) in Harnproben anerkannt. Die Nachweisgrenze (Durchschnitt der Leerwerte + 3 Standardabweichungen) bei Harnproben liegt bei 2,4 ppb fuer Clenbuterol. Diese Methode ermoeglicht, Konzentrationen von mindestens 5 ppb an Clenbuterol, Mabuterol und Cimaterol im Probenmaterial nachzuweisen. (orig.)

  14. Molecular identification of python species: development and validation of a novel assay for forensic investigations.

    Science.gov (United States)

    Ciavaglia, Sherryn A; Tobe, Shanan S; Donnellan, Stephen C; Henry, Julianne M; Linacre, Adrian M T

    2015-05-01

    Python snake species are often encountered in illegal activities and the question of species identity can be pertinent to such criminal investigations. Morphological identification of species of pythons can be confounded by many issues and molecular examination by DNA analysis can provide an alternative and objective means of identification. Our paper reports on the development and validation of a PCR primer pair that amplifies a segment of the mitochondrial cytochrome b gene that has been suggested previously as a good candidate locus for differentiating python species. We used this DNA region to perform species identification of pythons, even when the template DNA was of poor quality, as might be the case with forensic evidentiary items. Validation tests are presented to demonstrate the characteristics of the assay. Tests involved the cross-species amplification of this marker in non-target species, minimum amount of DNA template required, effects of degradation on product amplification and a blind trial to simulate a casework scenario that provided 100% correct identity. Our results demonstrate that this assay performs reliably and robustly on pythons and can be applied directly to forensic investigations where the presence of a species of python is in question. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  15. Development of the larval amphibian growth and development assay: Effects of benzophenone-2 exposure in Xenopus laevis from embryo to juvenile

    Science.gov (United States)

    The Larval Amphibian Growth and Development Assay (LAGDA) is a globally harmonized chemical testing guideline developed by the U.S. Environmental Protection Agency in collaboration with Japan’s Ministry of Environment to support risk assessment. The assay is employed as a ...

  16. Liquid metal reactor development. Development of LMR design technology

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young Cheol; Kim, Y. I.; Kim, Y. G.; Kim, E. K.; Song, H.; Chung, H. T.; Sim, Y. S.; Min, B. T.; Kim, Y. S.; Wi, M. H.; Yoo, B.; Lee, J. H.; Lee, H. Y.; Kim, J. B.; Koo, G. H.; Hahn, D. H.; Na, B. C.; Hwang, W.; Nam, C.; Ryu, W. S.; Lim, G. S.; Kim, D. H.; Kim, J. D.; Gil, C. S.

    1997-07-01

    This project was performed in five parts, the scope and contents of which are as follows: The nuclear data processing system was established and the KFS group constant library was improved and verified. Basic computation system was constructed by either developing or adding its function. Input/output (I/O) interface processing was developed to establish an integrated calculation system for LMR core nuclear rand thermal-hydraulic design and analysis. An experimental data analysis was performed to validate the constructed core neutronic calculation system. Using the established core calculation system and design technology, preliminary core design and performance analysis on the domestic LMR core design concept were carried out. To develop the basic technology of the LMR system analysis, LMR system behavior characteristics evaluation, thermal -fluid system analysis in the reactor pool, preliminary overall plant analysis and computer codes development have been performed. A porous model and simple one-dimensional model have been evaluated for the reactor pool analysis. The evaluation of the residual heat removal system on different design concepts has been also conducted. For the development of high temperature structural analysis, the heat transfer and thermal stress analyses were performed using finite element program with user subroutine that has been developed with an implementation of the Chaboche constitutive model for inelastic analysis capability, and the evaluation of creep-fatigue and ratcheting behavior of high temperature structure was carried out using this program. for development of the seismic isolation system and to predict the shear behavior for the laminated rubber bearing were established. And the behavior tests of isolation bearing and rubber specimens were carried out, and the seismic response tests for the isolation model structure were performed using the 30 ton shaking table. (author). 369 refs., 119 tabs., 320 figs.

  17. Wikipedia use: Risk for developing technology addiction

    OpenAIRE

    Sharma, Manoj Kumar

    2016-01-01

    The present case highlights the addictive potential of Wikipedia usage. The users approached a technology addiction clinic for the management of excessive use of technology. A clinical interview was used to elicit information about usages. It indicates the addictive use of Wikipedia and associated dysfunction in lifestyle. It has implication for promotion of healthy use of technology.

  18. Wikipedia use: Risk for developing technology addiction

    Directory of Open Access Journals (Sweden)

    Manoj Kumar Sharma

    2016-01-01

    Full Text Available The present case highlights the addictive potential of Wikipedia usage. The users approached a technology addiction clinic for the management of excessive use of technology. A clinical interview was used to elicit information about usages. It indicates the addictive use of Wikipedia and associated dysfunction in lifestyle. It has implication for promotion of healthy use of technology.

  19. Digital Technology and Student Cognitive Development

    Science.gov (United States)

    Cavanaugh, J. Michael; Giapponi, Catherine C.; Golden, Timothy D.

    2016-01-01

    Digital technology has proven a beguiling, some even venture addictive, presence in the lives of our 21st century (millennial) students. And while screen technology may offer select cognitive benefits, there is mounting evidence in the cognitive neuroscience literature that digital technology is restructuring the way our students read and think,…

  20. Wikipedia use: Risk for developing technology addiction.

    Science.gov (United States)

    Sharma, Manoj Kumar

    2016-01-01

    The present case highlights the addictive potential of Wikipedia usage. The users approached a technology addiction clinic for the management of excessive use of technology. A clinical interview was used to elicit information about usages. It indicates the addictive use of Wikipedia and associated dysfunction in lifestyle. It has implication for promotion of healthy use of technology.