Effects of a novel, selective, sigma1-ligand, MS-377, on phencyclidine-induced behaviour.

Science.gov (United States)

Takahashi, S; Takagi, K; Horikomi, K

2001-07-01

Phencyclidine (PCP)-induced head-weaving is inhibited by a novel selective sigma1-ligand, (R)-(+)-1-(4-chlorophenyl)-3-[4-(2-methoxyethyl)piperazin-1-yl]methyl-2-pyrrolidinone L-tartrate (MS-377), but not by dopamine D2 antagonists. In the present study, we examined the effects of two potent and selective sigma1-ligands, MS-377 and N,N-dipropyl-2-(4-methoxy-3-(2-phenylethoxy)phenyl) ethylamine (NE-100), on PCP-induced rearing behaviour, hyperlocomotion and ataxia in comparison with the currently available antipsychotic agents with affinity for D2 receptors, haloperidol, sultopride and risperidone. Male Wistar rats or ddY mice were administered MS-377, NE-100, haloperidol, sultopride or risperidone, and PCP was administered 60 min later (in the case of NE-100 10 min later). Rearing behaviour, hyperlocomotion and ataxia were examined 10 min after PCP administration. MS-377, haloperidol, sultopride and risperidone dose-dependently inhibited PCP-induced rearing and hyperlocomotion, but did not antagonize PCP-induced ataxia. In contrast, the other selective sigma1-ligand, NE-100, did not affect any of the PCP-induced behaviour patterns in this study. These results suggest that there are at least two types of ligands for sigma1-receptors and that some sigma1-ligands, including MS-377, have more comprehensive effects against PCP-induced abnormal behaviour than other sigma1-ligands or D2 antagonists.

Vermiform appendix: positions and length – a study of 377 cases and literature review

Directory of Open Access Journals (Sweden)

Sandro Cilindro de Souza

2015-10-01

Full Text Available Objective: Evaluation of the frequency of the relative positions and length of vermiform appendix in a group of corpses examined by the authors. Method: Dissection of 377 adult cadavers autopsied. Results and conclusions: Retrocecal: 43.5%; subcecal: 24.4% post-ileal: 14.3%, pelvic: 9.3%; paracecal: 5.8%; and pre-ileal appendices: 2.4%, other positions: 0.27%, mean length: 11.4 cm. Resumo: Objetivo: Avaliação da frequência das posições relativas e do comprimento do apêndice vermiforme em um grupo de cadáveres examinados pelos autores. Método: Dissecção de 377 cadáveres adultos necropsiados. Resultados e conclusões: Apêndices retrocecais: 43,5%, subcecais: 24,4%, pós-ileais: 14,3%, pélvico: 9,3%, paracecais: 5,8%, pré-ileais 2,4%, outras posições: 0,27%. Comprimento médio: 11,4 cm. Keywords: Vermiform appendix, Cecum, Anatomical variation, Appendicitis, Palavras-chave: Apêndice vermiforme, Ceco, Variação anatômica, Apendicite

A short comparison between mT2 and mCT

International Nuclear Information System (INIS)

Serna, Mario

2008-01-01

We compare m T2 with m CT ; both are kinematic variables designed to find relationships between masses of pair-produced new states with symmetric decay chains. We find that for massless visible particles m CT equals m T2 in a particular limit. We identify advantages and disadvantages to the use of each variable. Tovey's paper on m CT also introduced a powerful concept of extracting mass information from an analysis at intermediate stages of a symmetric decay chain. We suggest that m T2 is a better tool for performing this analysis than m CT due to m T2 's better properties under initial state radiation.

Topical ocular sodium 4-phenylbutyrate rescues glaucoma in a myocilin mouse model of primary open-angle glaucoma.

Science.gov (United States)

Zode, Gulab S; Bugge, Kevin E; Mohan, Kabhilan; Grozdanic, Sinisa D; Peters, Joseph C; Koehn, Demelza R; Anderson, Michael G; Kardon, Randy H; Stone, Edwin M; Sheffield, Val C

2012-03-01

Mutations in the myocilin gene (MYOC) are the most common known genetic cause of primary open-angle glaucoma (POAG). The purpose of this study was to determine whether topical ocular sodium 4-phenylbutyrate (PBA) treatment rescues glaucoma phenotypes in a mouse model of myocilin-associated glaucoma (Tg-MYOC(Y437H) mice). Tg-MYOC(Y437H) mice were treated with PBA eye drops (n = 10) or sterile PBS (n = 8) twice daily for 5 months. Long-term safety and effectiveness of topical PBA (0.2%) on glaucoma phenotypes were examined by measuring intraocular pressure (IOP) and pattern ERG (PERG), performing slit lamp evaluation of the anterior chamber, analyzing histologic sections of the anterior segment, and comparing myocilin levels in the aqueous humor and trabecular meshwork of Tg-MYOC(Y437H) mice. Tg-MYOC(Y437H) mice developed elevated IOP at 3 months of age when compared with wild-type (WT) littermates (n = 24; P < 0.0001). Topical PBA did not alter IOP in WT mice. However, it significantly reduced elevated IOP in Tg-MYOC(Y437H) mice to the level of WT mice. Topical PBA-treated Tg-MYOC(Y437H) mice also preserved PERG amplitudes compared with vehicle-treated Tg-MYOC(Y437H) mice. No structural abnormalities were observed in the anterior chamber of PBA-treated WT and Tg-MYOC(Y437H) mice. Analysis of the myocilin in the aqueous humor and TM revealed that PBA significantly improved the secretion of myocilin and reduced myocilin accumulation as well as endoplasmic reticulum (ER) stress in the TM of Tg-MYOC(Y437H) mice. Furthermore, topical PBA reduced IOP elevated by induction of ER stress via tunicamycin injections in WT mice. Topical ocular PBA reduces glaucomatous phenotypes in Tg-MYOC(Y437H) mice, most likely by reducing myocilin accumulation and ER stress in the TM. Topical ocular PBA could become a novel treatment for POAG patients with myocilin mutations.

Clinical utility of a 377 gene custom next-generation sequencing ...

Indian Academy of Sciences (India)

JEN BEVILACQUA

2017-07-26

Jul 26, 2017 ... Clinical utility of a 377 gene custom next-generation sequencing epilepsy panel ... number of genes, making it a very attractive option for a condition as .... clinical value of various test offerings to guide decision making.

Pulmonary 64-MDCT angiography with 50 mL of iodinated contrast material in an unselected patient population: a feasible protocol

Energy Technology Data Exchange (ETDEWEB)

Trad, Henrique Simao; Boasquevisque, Gustavo Santos; Giacometti, Tiago Rangon; Trad, Catherine Yang; Zoghbi Neto, Orlando Salomao; Trad, Clovis Simao, E-mail: hsimtrad@gmail.com [Central de Diagnostico Ribeirao Preto (CEDIRP), Ribeirao Preto, SP (Brazil)

2016-03-15

Objective: To propose a protocol for pulmonary angiography using 64-slice multidetector computed tomography (64-MDCT) with 50 mL of iodinated contrast material, in an unselected patient population, as well as to evaluate vascular enhancement and image quality. Materials and methods: We evaluated 29 patients (22-86 years of age). The body mass index ranged from 19.0 kg/m{sup 2} to 41.8 kg/m{sup 2}. Patients underwent pulmonary CT angiography in a 64-MDCT scanner, receiving 50 mL of iodinated contrast material via venous access at a rate of 4.5 mL/s. Bolus tracking was applied in the superior vena cava. Two experienced radiologists assessed image quality and vascular enhancement. Results: The mean density was 382 Hounsfield units (HU) for the pulmonary trunk; 379 and 377 HU for the right and left main pulmonary arteries, respectively; and 346 and 364 HU for the right and left inferior pulmonary arteries, respectively. In all patients, subsegmental arteries were analyzed. There were streak artifacts from contrast material in the superior vena cava in all patients. However, those artifacts did not impair the image analysis. Conclusion: Our findings suggest that pulmonary angiography using 64-MDCT with 50 mL of iodinated contrast can produce high quality images in unselected patient populations. (author)

Determination of Stress-Corrosion Cracking in Aluminum-Lithium Alloy ML377

Science.gov (United States)

Valek, Bryan C.

1995-01-01

The use of aluminum-lithium alloys for aerospace applications is currently being studied at NASA Langley Research Center's Metallic Materials Branch. The alloys in question will operate under stress in a corrosive environment. These conditions are ideal for the phenomena of Stress-Corrosion Cracking (SCC) to occur. The test procedure for SCC calls for alternate immersion and breaking load tests. These tests were optimized for the lab equipment and materials available in the Light Alloy lab. Al-Li alloy ML377 specimens were then subjected to alternate immersion and breaking load tests to determine residual strength and resistance to SCC. Corrosion morphology and microstructure were examined under magnification. Data shows that ML377 is highly resistant to stress-corrosion cracking.

Effects of sigma(1) receptor ligand, MS-377 on apomorphine- or phencyclidine-induced disruption of prepulse inhibition of acoustic startle in rats.

Science.gov (United States)

Yamada, S; Yamauchi, K; Hisatomi, S; Annoh, N; Tanaka, M

2000-08-25

To evaluate the antipsychotic property of a sigma(1) receptor ligand, (R)-(+)-1-(4-chlorophenyl)-3-¿4-(2-methoxyethyl)piperazin-1-yl¿ methyl-2-pyrrolidinone-L-tartrate (MS-377), an antagonistic effect of MS-377 on the disruption of prepulse inhibition (PPI) of the acoustic startle by apomorphine or phencyclidine (PCP) was investigated in rats. MS-377 antagonized the PCP-induced disruption of PPI. The ED(50) value of MS-377 for this effect was 0.66 mg/kg. In contrast, apomorphine-induced disruption of PPI was not attenuated by MS-377. These data indicate that the PCP-induced disruption of PPI in rats would be, at least partially, mediated by sigma receptors and MS-377 could be a novel anti-psychotic agent with clinical efficacy for the sensorimotor-gating deficit in schizophrenia.

21 CFR 1.377 - What definitions apply to this subpart?

Science.gov (United States)

2010-04-01

..., fruits, vegetables, fish, dairy products, eggs, raw agricultural commodities for use as food or components of food, animal feed, including pet food, food and feed ingredients and additives, including... 21 Food and Drugs 1 2010-04-01 2010-04-01 false What definitions apply to this subpart? 1.377...

MS-377, a novel selective sigma(1) receptor ligand, reverses phencyclidine-induced release of dopamine and serotonin in rat brain.

Science.gov (United States)

Takahashi, S; Horikomi, K; Kato, T

2001-09-21

A novel selective sigma(1) receptor ligand, (R)-(+)-1-(4-chlorophenyl)-3-[4-(2-methoxyethyl)piperazin-1-yl]methyl-2-pyrrolidinone L-tartrate (MS-377), inhibits phencyclidine (1-(1-phenylcyclohexyl)piperidine; PCP)-induced behaviors in animal models. In this study, we measured extracellular dopamine and serotonin levels in the rat brain after treatment with MS-377 alone, using in vivo microdialysis. We also examined the effects of MS-377 on extracellular dopamine and serotonin levels in the rat medial prefrontal cortex after treatment with PCP. MS-377 itself had no significant effects on dopamine release in the striatum (10 mg/kg, p.o.) nor on dopamine or serotonin release in the medial prefrontal cortex (1 and 10 mg/kg, p.o.). PCP (3 mg/kg, i.p.) markedly increased dopamine and serotonin release in the medial prefrontal cortex. MS-377 (1 mg/kg, p.o.), when administered 60 min prior to PCP, significantly attenuated this effect of PCP. These results suggest that the inhibitory effects of MS-377 on PCP-induced behaviors are partly mediated by inhibition of the increase in dopamine and serotonin release in the rat medial prefrontal cortex caused by PCP.

Socio-economical structure of population in Graboc të Poshtëm

OpenAIRE

, F. Isufi; , A. Sogojeva; , S. Bulliqi; , F. Humolli

2011-01-01

This paper is aimed at researching and comparing data derived from the survey, based on factors that influenced the orientation of the population in employment by age group and certain periods of time, 50-years of them, of last century until the nowadays, as well as by analyzing the changes that have occurred between these periods. Lower Graboc has a good geographical position, located at a convenient height of 530-550m above sea level, comprising geological characteristics and major natural ...

Liver Fat, Hepatic Enzymes, Alkaline Phosphatase and the Risk of Incident Type 2 Diabetes: A Prospective Study of 132,377 Adults

OpenAIRE

Chen, Sean Chun-Chang; Tsai, Shan Pou; Jhao, Jing-Yun; Jiang, Wun-Kai; Tsao, Chwen Keng; Chang, Ly-Yun

2017-01-01

Previous studies have reported inconsistent results of the associations of alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) with incident type 2 diabetes (diabetes hereafter). We aimed to resolve the controversy by taking nonalcoholic fatty liver disease (NAFLD) into account. The study population comprised 132,377 non-diabetic individuals (64,875 men and 67,502 women) aged 35?79 who had two or more health examinations dur...

Dynamics of 103K/N and 184M/V HIV-1 drug resistant populations: relative comparison in plasma virus RNA versus CD45RO+T cell proviral DNA

DEFF Research Database (Denmark)

Jakobsen, Martin Roelsgaard; Tolstrup, M; Bertelsen, L

2007-01-01

on real-time PCR and amplification refractory mutation system (ARMS). RESULTS: The 103N and 184V mutations were not detected in patients with stable low viremia. Patients previously exposed to mono or dual therapy often carried minor viral populations of either one or both mutations in plasma. The viral......BACKGROUND: Viral populations defined by 103K/N and 184M/V as linked or single mutations in the HIV-1 reverse transcriptase gene were investigated in plasma samples and compared with previous findings in the CD45RO(+)T cell compartment. OBJECTIVE: To develop an ARMS assay for plasma virions...... an unequal distribution of linked-mutation populations in plasma and CD45RO(+)T cells. Furthermore, the linked 103N-184V mutation may be more fit than the single 184V mutation and this linked population emerges rapidly under inadequate drug pressure. Udgivelsesdato: 2007-Jul...

34 CFR 377.21 - What selection criteria does the Secretary use?

Science.gov (United States)

2010-07-01

... funding; (4) The extent to which the applicant's plan identifies the numbers of eligible clients by type of disability and the number of eligible clients with severe disabilities who are available to... Section 377.21 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF...

Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies.

Directory of Open Access Journals (Sweden)

Shannon E Hill

Full Text Available Olfactomedin (OLF domains are found within extracellular, multidomain proteins in numerous tissues of multicellular organisms. Even though these proteins have been implicated in human disorders ranging from cancers to attention deficit disorder to glaucoma, little is known about their structure(s and function(s. Here we biophysically, biochemically, and structurally characterize OLF domains from H. sapiens olfactomedin-1 (npoh-OLF, also called noelin, pancortin, OLFM1, and hOlfA, and M. musculus gliomedin (glio-OLF, also called collomin, collmin, and CRG-L2, and compare them with available structures of myocilin (myoc-OLF recently reported by us and R. norvegicus glio-OLF and M. musculus latrophilin-3 (lat3-OLF by others. Although the five-bladed β-propeller architecture remains unchanged, numerous physicochemical characteristics differ among these OLF domains. First, npoh-OLF and glio-OLF exhibit prominent, yet distinct, positive surface charges and copurify with polynucleotides. Second, whereas npoh-OLF and myoc-OLF exhibit thermal stabilities typical of human proteins near 55°C, and most myoc-OLF variants are destabilized and highly prone to aggregation, glio-OLF is nearly 20°C more stable and significantly more resistant to chemical denaturation. Phylogenetically, glio-OLF is most similar to primitive OLFs, and structurally, glio-OLF is missing distinguishing features seen in OLFs such as the disulfide bond formed by N- and C- terminal cysteines, the sequestered Ca2+ ion within the propeller central hydrophilic cavity, and a key loop-stabilizing cation-π interaction on the top face of npoh-OLF and myoc-OLF. While deciphering the explicit biological functions, ligands, and binding partners for OLF domains will likely continue to be a challenging long-term experimental pursuit, we used structural insights gained here to generate a new antibody selective for myoc-OLF over npoh-OLF and glio-OLF as a first step in overcoming the impasse in

Measurement of the top quark mass in the dileptonic $\\mathrm{ t \\bar{t} }$ decay channel using the mass observables $M_{\\mathrm{ b }\\ell}$, $M_{\\mathrm{T}2}$, and $M_{\\mathrm{ b }\\ell\

CERN Document Server

Sirunyan, Albert M; CMS Collaboration; Adam, Wolfgang; Asilar, Ece; Bergauer, Thomas; Brandstetter, Johannes; Brondolin, Erica; Dragicevic, Marko; Erö, Janos; Flechl, Martin; Friedl, Markus; Fruehwirth, Rudolf; Ghete, Vasile Mihai; Hartl, Christian; Krammer, Natascha; Hrubec, Josef; Jeitler, Manfred; König, Axel; Krätschmer, Ilse; Liko, Dietrich; Matsushita, Takashi; Mikulec, Ivan; Rabady, Dinyar; Rad, Navid; Rahbaran, Babak; Rohringer, Herbert; Schieck, Jochen; Strauss, Josef; Waltenberger, Wolfgang; Wulz, Claudia-Elisabeth; Dvornikov, Oleg; Makarenko, Vladimir; Mossolov, Vladimir; Suarez Gonzalez, Juan; Zykunov, Vladimir; Shumeiko, Nikolai; Alderweireldt, Sara; De Wolf, Eddi A; Janssen, Xavier; Lauwers, Jasper; Van De Klundert, Merijn; Van Haevermaet, Hans; Van Mechelen, Pierre; Van Remortel, Nick; Van Spilbeeck, Alex; Abu Zeid, Shimaa; Blekman, Freya; D'Hondt, Jorgen; Daci, Nadir; De Bruyn, Isabelle; Deroover, Kevin; Lowette, Steven; Moortgat, Seth; Moreels, Lieselotte; Olbrechts, Annik; Python, Quentin; Skovpen, Kirill; Tavernier, Stefaan; Van Doninck, Walter; Van Mulders, Petra; Van Parijs, Isis; Brun, Hugues; Clerbaux, Barbara; De Lentdecker, Gilles; Delannoy, Hugo; Fasanella, Giuseppe; Favart, Laurent; Goldouzian, Reza; Grebenyuk, Anastasia; Karapostoli, Georgia; Lenzi, Thomas; Léonard, Alexandre; Luetic, Jelena; Maerschalk, Thierry; Marinov, Andrey; Randle-conde, Aidan; Seva, Tomislav; Vander Velde, Catherine; Vanlaer, Pascal; Vannerom, David; Yonamine, Ryo; Zenoni, Florian; Zhang, Fengwangdong; Cornelis, Tom; Dobur, Didar; Fagot, Alexis; Gul, Muhammad; Khvastunov, Illia; Poyraz, Deniz; Salva Diblen, Sinem; Schöfbeck, Robert; Tytgat, Michael; Van Driessche, Ward; Yazgan, Efe; Zaganidis, Nicolas; Bakhshiansohi, Hamed; Bondu, Olivier; Brochet, Sébastien; Bruno, Giacomo; Caudron, Adrien; De Visscher, Simon; Delaere, Christophe; Delcourt, Martin; Francois, Brieuc; Giammanco, Andrea; Jafari, Abideh; Komm, Matthias; Krintiras, Georgios; Lemaitre, Vincent; Magitteri, Alessio; Mertens, Alexandre; Musich, Marco; Piotrzkowski, Krzysztof; Quertenmont, Loic; Selvaggi, Michele; Vidal Marono, Miguel; Wertz, Sébastien; Beliy, Nikita; Aldá Júnior, Walter Luiz; Alves, Fábio Lúcio; Alves, Gilvan; Brito, Lucas; Hensel, Carsten; Moraes, Arthur; Pol, Maria Elena; Rebello Teles, Patricia; Belchior Batista Das Chagas, Ewerton; Carvalho, Wagner; Chinellato, Jose; Custódio, Analu; Melo Da Costa, Eliza; Da Silveira, Gustavo Gil; De Jesus Damiao, Dilson; De Oliveira Martins, Carley; Fonseca De Souza, Sandro; Huertas Guativa, Lina Milena; Malbouisson, Helena; Matos Figueiredo, Diego; Mora Herrera, Clemencia; Mundim, Luiz; Nogima, Helio; Prado Da Silva, Wanda Lucia; Santoro, Alberto; Sznajder, Andre; Tonelli Manganote, Edmilson José; Torres Da Silva De Araujo, Felipe; Vilela Pereira, Antonio; Ahuja, Sudha; Bernardes, Cesar Augusto; Dogra, Sunil; Tomei, Thiago; De Moraes Gregores, Eduardo; Mercadante, Pedro G; Moon, Chang-Seong; Novaes, Sergio F; Padula, Sandra; Romero Abad, David; Ruiz Vargas, José Cupertino; Aleksandrov, Aleksandar; Hadjiiska, Roumyana; Iaydjiev, Plamen; Rodozov, Mircho; Stoykova, Stefka; Sultanov, Georgi; Shopova, Mariana; Dimitrov, Anton; Glushkov, Ivan; Litov, Leander; Pavlov, Borislav; Petkov, Peicho; Fang, Wenxing; Ahmad, Muhammad; Bian, Jian-Guo; Chen, Guo-Ming; Chen, He-Sheng; Chen, Mingshui; Chen, Ye; Cheng, Tongguang; Jiang, Chun-Hua; Leggat, Duncan; Liu, Zhenan; Romeo, Francesco; Ruan, Manqi; Shaheen, Sarmad Masood; Spiezia, Aniello; Tao, Junquan; Wang, Chunjie; Wang, Zheng; Zhang, Huaqiao; Zhao, Jingzhou; Ban, Yong; Chen, Geng; Li, Qiang; Liu, Shuai; Mao, Yajun; Qian, Si-Jin; Wang, Dayong; Xu, Zijun; Avila, Carlos; Cabrera, Andrés; Chaparro Sierra, Luisa Fernanda; Florez, Carlos; Gomez, Juan Pablo; González Hernández, Carlos Felipe; Ruiz Alvarez, José David; Sanabria, Juan Carlos; Godinovic, Nikola; Lelas, Damir; Puljak, Ivica; Ribeiro Cipriano, Pedro M; Sculac, Toni; Antunovic, Zeljko; Kovac, Marko; Brigljevic, Vuko; Ferencek, Dinko; Kadija, Kreso; Mesic, Benjamin; Susa, Tatjana; Ather, Mohsan Waseem; Attikis, Alexandros; Mavromanolakis, Georgios; Mousa, Jehad; Nicolaou, Charalambos; Ptochos, Fotios; Razis, Panos A; Rykaczewski, Hans; Finger, Miroslav; Finger Jr, Michael; Carrera Jarrin, Edgar; Assran, Yasser; Elkafrawy, Tamer; Mahrous, Ayman; Kadastik, Mario; Perrini, Lucia; Raidal, Martti; Tiko, Andres; Veelken, Christian; Eerola, Paula; Pekkanen, Juska; Voutilainen, Mikko; Härkönen, Jaakko; Jarvinen, Terhi; Karimäki, Veikko; Kinnunen, Ritva; Lampén, Tapio; Lassila-Perini, Kati; Lehti, Sami; Lindén, Tomas; Luukka, Panja-Riina; Tuominiemi, Jorma; Tuovinen, Esa; Wendland, Lauri; Talvitie, Joonas; Tuuva, Tuure; Besancon, Marc; Couderc, Fabrice; Dejardin, Marc; Denegri, Daniel; Fabbro, Bernard; Faure, Jean-Louis; Favaro, Carlotta; Ferri, Federico; Ganjour, Serguei; Ghosh, Saranya; Givernaud, Alain; Gras, Philippe; Hamel de Monchenault, Gautier; Jarry, Patrick; Kucher, Inna; Locci, Elizabeth; Machet, Martina; Malcles, Julie; Rander, John; Rosowsky, André; Titov, Maksym; Abdulsalam, Abdulla; Antropov, Iurii; Baffioni, Stephanie; Beaudette, Florian; Busson, Philippe; Cadamuro, Luca; Chapon, Emilien; Charlot, Claude; Davignon, Olivier; Granier de Cassagnac, Raphael; Jo, Mihee; Lisniak, Stanislav; Miné, Philippe; Nguyen, Matthew; Ochando, Christophe; Ortona, Giacomo; Paganini, Pascal; Pigard, Philipp; Regnard, Simon; Salerno, Roberto; Sirois, Yves; Stahl Leiton, Andre Govinda; Strebler, Thomas; Yilmaz, Yetkin; Zabi, Alexandre; Zghiche, Amina; Agram, Jean-Laurent; Andrea, Jeremy; Bloch, Daniel; Brom, Jean-Marie; Buttignol, Michael; Chabert, Eric Christian; Chanon, Nicolas; Collard, Caroline; Conte, Eric; Coubez, Xavier; Fontaine, Jean-Charles; Gelé, Denis; Goerlach, Ulrich; Le Bihan, Anne-Catherine; Van Hove, Pierre; Gadrat, Sébastien; Beauceron, Stephanie; Bernet, Colin; Boudoul, Gaelle; Carrillo Montoya, Camilo Andres; Chierici, Roberto; Contardo, Didier; Courbon, Benoit; Depasse, Pierre; El Mamouni, Houmani; Fay, Jean; Finco, Linda; Gascon, Susan; Gouzevitch, Maxime; Grenier, Gérald; Ille, Bernard; Lagarde, Francois; Laktineh, Imad Baptiste; Lethuillier, Morgan; Mirabito, Laurent; Pequegnot, Anne-Laure; Perries, Stephane; Popov, Andrey; Sordini, Viola; Vander Donckt, Muriel; Verdier, Patrice; Viret, Sébastien; Khvedelidze, Arsen; Tsamalaidze, Zviad; Autermann, Christian; Beranek, Sarah; Feld, Lutz; Kiesel, Maximilian Knut; Klein, Katja; Lipinski, Martin; Preuten, Marius; Schomakers, Christian; Schulz, Johannes; Verlage, Tobias; Albert, Andreas; Brodski, Michael; Dietz-Laursonn, Erik; Duchardt, Deborah; Endres, Matthias; Erdmann, Martin; Erdweg, Sören; Esch, Thomas; Fischer, Robert; Güth, Andreas; Hamer, Matthias; Hebbeker, Thomas; Heidemann, Carsten; Hoepfner, Kerstin; Knutzen, Simon; Merschmeyer, Markus; Meyer, Arnd; Millet, Philipp; Mukherjee, Swagata; Olschewski, Mark; Padeken, Klaas; Pook, Tobias; Radziej, Markus; Reithler, Hans; Rieger, Marcel; Scheuch, Florian; Sonnenschein, Lars; Teyssier, Daniel; Thüer, Sebastian; Cherepanov, Vladimir; Flügge, Günter; Kargoll, Bastian; Kress, Thomas; Künsken, Andreas; Lingemann, Joschka; Müller, Thomas; Nehrkorn, Alexander; Nowack, Andreas; Pistone, Claudia; Pooth, Oliver; Stahl, Achim; Aldaya Martin, Maria; Arndt, Till; Asawatangtrakuldee, Chayanit; Beernaert, Kelly; Behnke, Olaf; Behrens, Ulf; Bin Anuar, Afiq Aizuddin; Borras, Kerstin; Campbell, Alan; Connor, Patrick; Contreras-Campana, Christian; Costanza, Francesco; Diez Pardos, Carmen; Dolinska, Ganna; Eckerlin, Guenter; Eckstein, Doris; Eichhorn, Thomas; Eren, Engin; Gallo, Elisabetta; Garay Garcia, Jasone; Geiser, Achim; Gizhko, Andrii; Grados Luyando, Juan Manuel; Grohsjean, Alexander; Gunnellini, Paolo; Harb, Ali; Hauk, Johannes; Hempel, Maria; Jung, Hannes; Kalogeropoulos, Alexis; Karacheban, Olena; Kasemann, Matthias; Keaveney, James; Kleinwort, Claus; Korol, Ievgen; Krücker, Dirk; Lange, Wolfgang; Lelek, Aleksandra; Lenz, Teresa; Leonard, Jessica; Lipka, Katerina; Lobanov, Artur; Lohmann, Wolfgang; Mankel, Rainer; Melzer-Pellmann, Isabell-Alissandra; Meyer, Andreas Bernhard; Mittag, Gregor; Mnich, Joachim; Mussgiller, Andreas; Pitzl, Daniel; Placakyte, Ringaile; Raspereza, Alexei; Roland, Benoit; Sahin, Mehmet Özgür; Saxena, Pooja; Schoerner-Sadenius, Thomas; Spannagel, Simon; Stefaniuk, Nazar; Van Onsem, Gerrit Patrick; Walsh, Roberval; Wissing, Christoph; Blobel, Volker; Centis Vignali, Matteo; Draeger, Arne-Rasmus; Dreyer, Torben; Garutti, Erika; Gonzalez, Daniel; Haller, Johannes; Hoffmann, Malte; Junkes, Alexandra; Klanner, Robert; Kogler, Roman; Kovalchuk, Nataliia; Kurz, Simon; Lapsien, Tobias; Marchesini, Ivan; Marconi, Daniele; Meyer, Mareike; Niedziela, Marek; Nowatschin, Dominik; Pantaleo, Felice; Peiffer, Thomas; Perieanu, Adrian; Scharf, Christian; Schleper, Peter; Schmidt, Alexander; Schumann, Svenja; Schwandt, Joern; Sonneveld, Jory; Stadie, Hartmut; Steinbrück, Georg; Stober, Fred-Markus Helmut; Stöver, Marc; Tholen, Heiner; Troendle, Daniel; Usai, Emanuele; Vanelderen, Lukas; Vanhoefer, Annika; Vormwald, Benedikt; Akbiyik, Melike; Barth, Christian; Baur, Sebastian; Baus, Colin; Berger, Joram; Butz, Erik; Caspart, René; Chwalek, Thorsten; Colombo, Fabio; De Boer, Wim; Dierlamm, Alexander; Fink, Simon; Freund, Benedikt; Friese, Raphael; Giffels, Manuel; Gilbert, Andrew; Goldenzweig, Pablo; Haitz, Dominik; Hartmann, Frank; Heindl, Stefan Michael; Husemann, Ulrich; Kassel, Florian; Katkov, Igor; Kudella, Simon; Mildner, Hannes; Mozer, Matthias Ulrich; Müller, Thomas; Plagge, Michael; Quast, Gunter; Rabbertz, Klaus; Röcker, Steffen; Roscher, Frank; Schröder, Matthias; Shvetsov, Ivan; Sieber, Georg; Simonis, Hans-Jürgen; Ulrich, Ralf; Wayand, Stefan; Weber, Marc; Weiler, Thomas; Williamson, Shawn; Wöhrmann, Clemens; Wolf, Roger; Anagnostou, Georgios; Daskalakis, Georgios; Geralis, Theodoros; Giakoumopoulou, Viktoria Athina; Kyriakis, Aristotelis; Loukas, Demetrios; Topsis-Giotis, Iasonas; Kesisoglou, Stilianos; Panagiotou, Apostolos; Saoulidou, Niki; Tziaferi, Eirini; Kousouris, Konstantinos; Evangelou, Ioannis; Flouris, Giannis; Foudas, Costas; Kokkas, Panagiotis; Loukas, Nikitas; Manthos, Nikolaos; Papadopoulos, Ioannis; Paradas, Evangelos; Filipovic, Nicolas; Pasztor, Gabriella; Bencze, Gyorgy; Hajdu, Csaba; Horvath, Dezso; Sikler, Ferenc; Veszpremi, Viktor; Vesztergombi, Gyorgy; Zsigmond, Anna Julia; Beni, Noemi; Czellar, Sandor; Karancsi, János; Makovec, Alajos; Molnar, Jozsef; Szillasi, Zoltan; Bartók, Márton; Raics, Peter; Trocsanyi, Zoltan Laszlo; Ujvari, Balazs; Choudhury, Somnath; Komaragiri, Jyothsna Rani; Bahinipati, Seema; Bhowmik, Sandeep; Mal, Prolay; Mandal, Koushik; Nayak, Aruna; Sahoo, Deepak Kumar; Sahoo, Niladribihari; Swain, Sanjay Kumar; Bansal, Sunil; Beri, Suman Bala; Bhatnagar, Vipin; Chawla, Ridhi; Bhawandeep, Bhawandeep; Kalsi, Amandeep Kaur; Kaur, Anterpreet; Kaur, Manjit; Kumar, Ramandeep; Kumari, Priyanka; Mehta, Ankita; Mittal, Monika; Singh, Jasbir; Walia, Genius; Kumar, Ashok; Bhardwaj, Ashutosh; Choudhary, Brajesh C; Garg, Rocky Bala; Keshri, Sumit; Kumar, Ajay; Malhotra, Shivali; Naimuddin, Md; Ranjan, Kirti; Sharma, Ramkrishna; Sharma, Varun; Bhattacharya, Rajarshi; Bhattacharya, Satyaki; Chatterjee, Kalyanmoy; Dey, Sourav; Dutt, Suneel; Dutta, Suchandra; Ghosh, Shamik; Majumdar, Nayana; Modak, Atanu; Mondal, Kuntal; Mukhopadhyay, Supratik; Nandan, Saswati; Purohit, Arnab; Roy, Ashim; Roy, Debarati; Roy Chowdhury, Suvankar; Sarkar, Subir; Sharan, Manoj; Thakur, Shalini; Behera, Prafulla Kumar; Chudasama, Ruchi; Dutta, Dipanwita; Jha, Vishwajeet; Kumar, Vineet; Mohanty, Ajit Kumar; Netrakanti, Pawan Kumar; Pant, Lalit Mohan; Shukla, Prashant; Topkar, Anita; Aziz, Tariq; Dugad, Shashikant; Kole, Gouranga; Mahakud, Bibhuprasad; Mitra, Soureek; Mohanty, Gagan Bihari; Parida, Bibhuti; Sur, Nairit; Sutar, Bajrang; Banerjee, Sudeshna; Dewanjee, Ram Krishna; Ganguly, Sanmay; Guchait, Monoranjan; Jain, Sandhya; Kumar, Sanjeev; Maity, Manas; Majumder, Gobinda; Mazumdar, Kajari; Sarkar, Tanmay; Wickramage, Nadeesha; Chauhan, Shubhanshu; Dube, Sourabh; Hegde, Vinay; Kapoor, Anshul; Kothekar, Kunal; Pandey, Shubham; Rane, Aditee; Sharma, Seema; Chenarani, Shirin; Eskandari Tadavani, Esmaeel; Etesami, Seyed Mohsen; Khakzad, Mohsen; Mohammadi Najafabadi, Mojtaba; Naseri, Mohsen; Paktinat Mehdiabadi, Saeid; Rezaei Hosseinabadi, Ferdos; Safarzadeh, Batool; Zeinali, Maryam; Felcini, Marta; Grunewald, Martin; Abbrescia, Marcello; Calabria, Cesare; Caputo, Claudio; Colaleo, Anna; Creanza, Donato; Cristella, Leonardo; De Filippis, Nicola; De Palma, Mauro; Fiore, Luigi; Iaselli, Giuseppe; Maggi, Giorgio; Maggi, Marcello; Miniello, Giorgia; My, Salvatore; Nuzzo, Salvatore; Pompili, Alexis; Pugliese, Gabriella; Radogna, Raffaella; Ranieri, Antonio; Selvaggi, Giovanna; Sharma, Archana; Silvestris, Lucia; Venditti, Rosamaria; Verwilligen, Piet; Abbiendi, Giovanni; Battilana, Carlo; Bonacorsi, Daniele; Braibant-Giacomelli, Sylvie; Brigliadori, Luca; Campanini, Renato; Capiluppi, Paolo; Castro, Andrea; Cavallo, Francesca Romana; Chhibra, Simranjit Singh; Codispoti, Giuseppe; Cuffiani, Marco; Dallavalle, Gaetano-Marco; Fabbri, Fabrizio; Fanfani, Alessandra; Fasanella, Daniele; Giacomelli, Paolo; Grandi, Claudio; Guiducci, Luigi; Marcellini, Stefano; Masetti, Gianni; Montanari, Alessandro; Navarria, Francesco; Perrotta, Andrea; Rossi, Antonio; Rovelli, Tiziano; Siroli, Gian Piero; Tosi, Nicolò; Albergo, Sebastiano; Costa, Salvatore; Di Mattia, Alessandro; Giordano, Ferdinando; Potenza, Renato; Tricomi, Alessia; Tuve, Cristina; Barbagli, Giuseppe; Ciulli, Vitaliano; Civinini, Carlo; D'Alessandro, Raffaello; Focardi, Ettore; Lenzi, Piergiulio; Meschini, Marco; Paoletti, Simone; Russo, Lorenzo; Sguazzoni, Giacomo; Strom, Derek; Viliani, Lorenzo; Benussi, Luigi; Bianco, Stefano; Fabbri, Franco; Piccolo, Davide; Primavera, Federica; Calvelli, Valerio; Ferro, Fabrizio; Monge, Maria Roberta; Robutti, Enrico; Tosi, Silvano; Brianza, Luca; Brivio, Francesco; Ciriolo, Vincenzo; Dinardo, Mauro Emanuele; Fiorendi, Sara; Gennai, Simone; Ghezzi, Alessio; Govoni, Pietro; Malberti, Martina; Malvezzi, Sandra; Manzoni, Riccardo Andrea; Menasce, Dario; Moroni, Luigi; Paganoni, Marco; Pedrini, Daniele; Pigazzini, Simone; Ragazzi, Stefano; Tabarelli de Fatis, Tommaso; Buontempo, Salvatore; Cavallo, Nicola; De Nardo, Guglielmo; Di Guida, Salvatore; Esposito, Marco; Fabozzi, Francesco; Fienga, Francesco; Iorio, Alberto Orso Maria; Lanza, Giuseppe; Lista, Luca; Meola, Sabino; Paolucci, Pierluigi; Sciacca, Crisostomo; Thyssen, Filip; Azzi, Patrizia; Bacchetta, Nicola; Benato, Lisa; Bisello, Dario; Boletti, Alessio; Carlin, Roberto; Checchia, Paolo; Dall'Osso, Martino; De Castro Manzano, Pablo; Dorigo, Tommaso; Dosselli, Umberto; Gasparini, Fabrizio; Gasparini, Ugo; Lacaprara, Stefano; Margoni, Martino; Meneguzzo, Anna Teresa; Pazzini, Jacopo; Pozzobon, Nicola; Ronchese, Paolo; Rossin, Roberto; Simonetto, Franco; Torassa, Ezio; Ventura, Sandro; Zanetti, Marco; Zotto, Pierluigi; Zumerle, Gianni; Braghieri, Alessandro; Fallavollita, Francesco; Magnani, Alice; Montagna, Paolo; Ratti, Sergio P; Re, Valerio; Ressegotti, Martina; Riccardi, Cristina; Salvini, Paola; Vai, Ilaria; Vitulo, Paolo; Alunni Solestizi, Luisa; Bilei, Gian Mario; Ciangottini, Diego; Fanò, Livio; Lariccia, Paolo; Leonardi, Roberto; Mantovani, Giancarlo; Mariani, Valentina; Menichelli, Mauro; Saha, Anirban; Santocchia, Attilio; Androsov, Konstantin; Azzurri, Paolo; Bagliesi, Giuseppe; Bernardini, Jacopo; Boccali, Tommaso; Castaldi, Rino; Ciocci, Maria Agnese; Dell'Orso, Roberto; Fedi, Giacomo; Giassi, Alessandro; Grippo, Maria Teresa; Ligabue, Franco; Lomtadze, Teimuraz; Martini, Luca; Messineo, Alberto; Palla, Fabrizio; Rizzi, Andrea; Savoy-Navarro, Aurore; Spagnolo, Paolo; Tenchini, Roberto; Tonelli, Guido; Venturi, Andrea; Verdini, Piero Giorgio; Barone, Luciano; Cavallari, Francesca; Cipriani, Marco; Del Re, Daniele; Diemoz, Marcella; Gelli, Simone; Longo, Egidio; Margaroli, Fabrizio; Marzocchi, Badder; Meridiani, Paolo; Organtini, Giovanni; Paramatti, Riccardo; Preiato, Federico; Rahatlou, Shahram; Rovelli, Chiara; Santanastasio, Francesco; Amapane, Nicola; Arcidiacono, Roberta; Argiro, Stefano; Arneodo, Michele; Bartosik, Nazar; Bellan, Riccardo; Biino, Cristina; Cartiglia, Nicolo; Cenna, Francesca; Costa, Marco; Covarelli, Roberto; Degano, Alessandro; Demaria, Natale; Kiani, Bilal; Mariotti, Chiara; Maselli, Silvia; Migliore, Ernesto; Monaco, Vincenzo; Monteil, Ennio; Monteno, Marco; Obertino, Maria Margherita; Pacher, Luca; Pastrone, Nadia; Pelliccioni, Mario; Pinna Angioni, Gian Luca; Ravera, Fabio; Romero, Alessandra; Ruspa, Marta; Sacchi, Roberto; Shchelina, Ksenia; Sola, Valentina; Solano, Ada; Staiano, Amedeo; Traczyk, Piotr; Belforte, Stefano; Casarsa, Massimo; Cossutti, Fabio; Della Ricca, Giuseppe; Zanetti, Anna; Kim, Dong Hee; Kim, Gui Nyun; Kim, Min Suk; Lee, Jeongeun; Lee, Sangeun; Lee, Seh Wook; Oh, Young Do; Sekmen, Sezen; Son, Dong-Chul; Yang, Yu Chul; Lee, Ari; Kim, Hyunchul; Brochero Cifuentes, Javier Andres; Kim, Tae Jeong; Cho, Sungwoong; Choi, Suyong; Go, Yeonju; Gyun, Dooyeon; Ha, Seungkyu; Hong, Byung-Sik; Jo, Youngkwon; Kim, Yongsun; Lee, Kisoo; Lee, Kyong Sei; Lee, Songkyo; Lim, Jaehoon; Park, Sung Keun; Roh, Youn; Almond, John; Kim, Junho; Lee, Haneol; Oh, Sung Bin; Radburn-Smith, Benjamin Charles; Seo, Seon-hee; Yang, Unki; Yoo, Hwi Dong; Yu, Geum Bong; Choi, Minkyoo; Kim, Hyunyong; Kim, Ji Hyun; Lee, Jason Sang Hun; Park, Inkyu; Ryu, Geonmo; Ryu, Min Sang; Choi, Young-Il; Goh, Junghwan; Hwang, Chanwook; Lee, Jongseok; Yu, Intae; Dudenas, Vytautas; Juodagalvis, Andrius; Vaitkus, Juozas; Ahmed, Ijaz; Ibrahim, Zainol Abidin; Md Ali, Mohd Adli Bin; Mohamad Idris, Faridah; Wan Abdullah, Wan Ahmad Tajuddin; Yusli, Mohd Nizam; Zolkapli, Zukhaimira; Castilla-Valdez, Heriberto; De La Cruz-Burelo, Eduard; Heredia-De La Cruz, Ivan; Hernandez-Almada, Alberto; Lopez-Fernandez, Ricardo; Magaña Villalba, Ricardo; Mejia Guisao, Jhovanny; Sánchez Hernández, Alberto; Carrillo Moreno, Salvador; Oropeza Barrera, Cristina; Vazquez Valencia, Fabiola; Carpinteyro, Severiano; Pedraza, Isabel; Salazar Ibarguen, Humberto Antonio; Uribe Estrada, Cecilia; Morelos Pineda, Antonio; Krofcheck, David; Butler, Philip H; Ahmad, Ashfaq; Ahmad, Muhammad; Hassan, Qamar; Hoorani, Hafeez R; Khan, Wajid Ali; Saddique, Asif; Shah, Mehar Ali; Shoaib, Muhammad; Waqas, Muhammad; Bialkowska, Helena; Bluj, Michal; Boimska, Bozena; Frueboes, Tomasz; Górski, Maciej; Kazana, Malgorzata; Nawrocki, Krzysztof; Romanowska-Rybinska, Katarzyna; Szleper, Michal; Zalewski, Piotr; Bunkowski, Karol; Byszuk, Adrian; Doroba, Krzysztof; Kalinowski, Artur; Konecki, Marcin; Krolikowski, Jan; Misiura, Maciej; Olszewski, Michal; Pyskir, Andrzej; Walczak, Marek; Bargassa, Pedrame; Beirão Da Cruz E Silva, Cristóvão; Calpas, Betty; Di Francesco, Agostino; Faccioli, Pietro; Gallinaro, Michele; Hollar, Jonathan; Leonardo, Nuno; Lloret Iglesias, Lara; Nemallapudi, Mythra Varun; Seixas, Joao; Toldaiev, Oleksii; Vadruccio, Daniele; Varela, Joao; Afanasiev, Serguei; Bunin, Pavel; Gavrilenko, Mikhail; Golutvin, Igor; Gorbunov, Ilya; Kamenev, Alexey; Karjavin, Vladimir; Lanev, Alexander; Malakhov, Alexander; Matveev, Viktor; Palichik, Vladimir; Perelygin, Victor; Shmatov, Sergey; Shulha, Siarhei; Skatchkov, Nikolai; Smirnov, Vitaly; Voytishin, Nikolay; Zarubin, Anatoli; Chtchipounov, Leonid; Golovtsov, Victor; Ivanov, Yury; Kim, Victor; Kuznetsova, Ekaterina; Murzin, Victor; Oreshkin, Vadim; Sulimov, Valentin; Vorobyev, Alexey; Andreev, Yuri; Dermenev, Alexander; Gninenko, Sergei; Golubev, Nikolai; Karneyeu, Anton; Kirsanov, Mikhail; Krasnikov, Nikolai; Pashenkov, Anatoli; Tlisov, Danila; Toropin, Alexander; Epshteyn, Vladimir; Gavrilov, Vladimir; Lychkovskaya, Natalia; Popov, Vladimir; Pozdnyakov, Ivan; Safronov, Grigory; Spiridonov, Alexander; Toms, Maria; Vlasov, Evgueni; Zhokin, Alexander; Aushev, Tagir; Bylinkin, Alexander; Chadeeva, Marina; Markin, Oleg; Tarkovskii, Evgenii; Andreev, Vladimir; Azarkin, Maksim; Dremin, Igor; Kirakosyan, Martin; Leonidov, Andrey; Terkulov, Adel; Baskakov, Alexey; Belyaev, Andrey; Boos, Edouard; Bunichev, Viacheslav; Dubinin, Mikhail; Dudko, Lev; Klyukhin, Vyacheslav; Kodolova, Olga; Korneeva, Natalia; Lokhtin, Igor; Miagkov, Igor; Obraztsov, Stepan; Perfilov, Maxim; Savrin, Viktor; Volkov, Petr; Blinov, Vladimir; Skovpen, Yuri; Shtol, Dmitry; Azhgirey, Igor; Bayshev, Igor; Bitioukov, Sergei; Elumakhov, Dmitry; Kachanov, Vassili; Kalinin, Alexey; Konstantinov, Dmitri; Krychkine, Victor; Petrov, Vladimir; Ryutin, Roman; Sobol, Andrei; Troshin, Sergey; Tyurin, Nikolay; Uzunian, Andrey; Volkov, Alexey; Adzic, Petar; Cirkovic, Predrag; Devetak, Damir; Dordevic, Milos; Milosevic, Jovan; Rekovic, Vladimir; Alcaraz Maestre, Juan; Barrio Luna, Mar; Calvo, Enrique; Cerrada, Marcos; Chamizo Llatas, Maria; Colino, Nicanor; De La Cruz, Begona; Delgado Peris, Antonio; Escalante Del Valle, Alberto; Fernandez Bedoya, Cristina; Fernández Ramos, Juan Pablo; Flix, Jose; Fouz, Maria Cruz; Garcia-Abia, Pablo; Gonzalez Lopez, Oscar; Goy Lopez, Silvia; Hernandez, Jose M; Josa, Maria Isabel; Navarro De Martino, Eduardo; Pérez-Calero Yzquierdo, Antonio María; Puerta Pelayo, Jesus; Quintario Olmeda, Adrián; Redondo, Ignacio; Romero, Luciano; Senghi Soares, Mara; de Trocóniz, Jorge F; Missiroli, Marino; Moran, Dermot; Cuevas, Javier; Erice, Carlos; Fernandez Menendez, Javier; Gonzalez Caballero, Isidro; González Fernández, Juan Rodrigo; Palencia Cortezon, Enrique; Sanchez Cruz, Sergio; Suárez Andrés, Ignacio; Vischia, Pietro; Vizan Garcia, Jesus Manuel; Cabrillo, Iban Jose; Calderon, Alicia; Curras, Esteban; Fernandez, Marcos; Garcia-Ferrero, Juan; Gomez, Gervasio; Lopez Virto, Amparo; Marco, Jesus; Martinez Rivero, Celso; Matorras, Francisco; Piedra Gomez, Jonatan; Rodrigo, Teresa; Ruiz-Jimeno, Alberto; Scodellaro, Luca; Trevisani, Nicolò; Vila, Ivan; Vilar Cortabitarte, Rocio; Abbaneo, Duccio; Auffray, Etiennette; Auzinger, Georg; Baillon, Paul; Ball, Austin; Barney, David; Bloch, Philippe; Bocci, Andrea; Botta, Cristina; Camporesi, Tiziano; Castello, Roberto; Cepeda, Maria; Cerminara, Gianluca; Chen, Yi; Cimmino, Anna; D'Enterria, David; Dabrowski, Anne; Daponte, Vincenzo; David Tinoco Mendes, Andre; De Gruttola, Michele; De Roeck, Albert; Di Marco, Emanuele; Dobson, Marc; Dorney, Brian; Du Pree, Tristan; Duggan, Daniel; Dünser, Marc; Dupont, Niels; Elliott-Peisert, Anna; Everaerts, Pieter; Fartoukh, Stephane; Franzoni, Giovanni; Fulcher, Jonathan; Funk, Wolfgang; Gigi, Dominique; Gill, Karl; Girone, Maria; Glege, Frank; Gulhan, Doga; Gundacker, Stefan; Guthoff, Moritz; Harris, Philip; Hegeman, Jeroen; Innocente, Vincenzo; Janot, Patrick; Kieseler, Jan; Kirschenmann, Henning; Knünz, Valentin; Kornmayer, Andreas; Kortelainen, Matti J; Krammer, Manfred; Lange, Clemens; Lecoq, Paul; Lourenco, Carlos; Lucchini, Marco Toliman; Malgeri, Luca; Mannelli, Marcello; Martelli, Arabella; Meijers, Frans; Merlin, Jeremie Alexandre; Mersi, Stefano; Meschi, Emilio; Milenovic, Predrag; Moortgat, Filip; Morovic, Srecko; Mulders, Martijn; Neugebauer, Hannes; Orfanelli, Styliani; Orsini, Luciano; Pape, Luc; Perez, Emmanuel; Peruzzi, Marco; Petrilli, Achille; Petrucciani, Giovanni; Pfeiffer, Andreas; Pierini, Maurizio; Racz, Attila; Reis, Thomas; Rolandi, Gigi; Rovere, Marco; Sakulin, Hannes; Sauvan, Jean-Baptiste; Schäfer, Christoph; Schwick, Christoph; Seidel, Markus; Sharma, Archana; Silva, Pedro; Sphicas, Paraskevas; Steggemann, Jan; Stoye, Markus; Takahashi, Yuta; Tosi, Mia; Treille, Daniel; Triossi, Andrea; Tsirou, Andromachi; Veckalns, Viesturs; Veres, Gabor Istvan; Verweij, Marta; Wardle, Nicholas; Wöhri, Hermine Katharina; Zagozdzinska, Agnieszka; Zeuner, Wolfram Dietrich; Bertl, Willi; Deiters, Konrad; Erdmann, Wolfram; Horisberger, Roland; Ingram, Quentin; Kaestli, Hans-Christian; Kotlinski, Danek; Langenegger, Urs; Rohe, Tilman; Wiederkehr, Stephan Albert; Bachmair, Felix; Bäni, Lukas; Bianchini, Lorenzo; Casal, Bruno; Dissertori, Günther; Dittmar, Michael; Donegà, Mauro; Grab, Christoph; Heidegger, Constantin; Hits, Dmitry; Hoss, Jan; Kasieczka, Gregor; Lustermann, Werner; Mangano, Boris; Marionneau, Matthieu; Martinez Ruiz del Arbol, Pablo; Masciovecchio, Mario; Meinhard, Maren Tabea; Meister, Daniel; Micheli, Francesco; Musella, Pasquale; Nessi-Tedaldi, Francesca; Pandolfi, Francesco; Pata, Joosep; Pauss, Felicitas; Perrin, Gaël; Perrozzi, Luca; Quittnat, Milena; Rossini, Marco; Schönenberger, Myriam; Starodumov, Andrei; Tavolaro, Vittorio Raoul; Theofilatos, Konstantinos; Wallny, Rainer; Aarrestad, Thea Klaeboe; Amsler, Claude; Caminada, Lea; Canelli, Maria Florencia; De Cosa, Annapaola; Donato, Silvio; Galloni, Camilla; Hinzmann, Andreas; Hreus, Tomas; Kilminster, Benjamin; Ngadiuba, Jennifer; Pinna, Deborah; Rauco, Giorgia; Robmann, Peter; Salerno, Daniel; Seitz, Claudia; Yang, Yong; Zucchetta, Alberto; Candelise, Vieri; Doan, Thi Hien; Jain, Shilpi; Khurana, Raman; Konyushikhin, Maxim; Kuo, Chia-Ming; Lin, Willis; Pozdnyakov, Andrey; Yu, Shin-Shan; Kumar, Arun; Chang, Paoti; Chang, You-Hao; Chao, Yuan; Chen, Kai-Feng; Chen, Po-Hsun; Fiori, Francesco; Hou, George Wei-Shu; Hsiung, Yee; Liu, Yueh-Feng; Lu, Rong-Shyang; Miñano Moya, Mercedes; Paganis, Efstathios; Psallidas, Andreas; Tsai, Jui-fa; Asavapibhop, Burin; Singh, Gurpreet; Srimanobhas, Norraphat; Suwonjandee, Narumon; Adiguzel, Aytul; Bakirci, Mustafa Numan; Boran, Fatma; Cerci, Salim; Damarseckin, Serdal; Demiroglu, Zuhal Seyma; Dozen, Candan; Dumanoglu, Isa; Girgis, Semiray; Gokbulut, Gul; Guler, Yalcin; Hos, Ilknur; Kangal, Evrim Ersin; Kara, Ozgun; Kayis Topaksu, Aysel; Kiminsu, Ugur; Oglakci, Mehmet; Onengut, Gulsen; Ozdemir, Kadri; Tali, Bayram; Turkcapar, Semra; Zorbakir, Ibrahim Soner; Zorbilmez, Caglar; Bilin, Bugra; Bilmis, Selcuk; Isildak, Bora; Karapinar, Guler; Yalvac, Metin; Zeyrek, Mehmet; Gülmez, Erhan; Kaya, Mithat; Kaya, Ozlem; Yetkin, Elif Asli; Yetkin, Taylan; Cakir, Altan; Cankocak, Kerem; Sen, Sercan; Grynyov, Boris; Levchuk, Leonid; Sorokin, Pavel; Aggleton, Robin; Ball, Fionn; Beck, Lana; Brooke, James John; Burns, Douglas; Clement, Emyr; Cussans, David; Flacher, Henning; Goldstein, Joel; Grimes, Mark; Heath, Greg P; Heath, Helen F; Jacob, Jeson; Kreczko, Lukasz; Lucas, Chris; Newbold, Dave M; Paramesvaran, Sudarshan; Poll, Anthony; Sakuma, Tai; Seif El Nasr-storey, Sarah; Smith, Dominic; Smith, Vincent J; Bell, Ken W; Belyaev, Alexander; Brew, Christopher; Brown, Robert M; Calligaris, Luigi; Cieri, Davide; Cockerill, David JA; Coughlan, John A; Harder, Kristian; Harper, Sam; Olaiya, Emmanuel; Petyt, David; Shepherd-Themistocleous, Claire; Thea, Alessandro; Tomalin, Ian R; Williams, Thomas; Baber, Mark; Bainbridge, Robert; Buchmuller, Oliver; Bundock, Aaron; Casasso, Stefano; Citron, Matthew; Colling, David; Corpe, Louie; Dauncey, Paul; Davies, Gavin; De Wit, Adinda; Della Negra, Michel; Di Maria, Riccardo; Dunne, Patrick; Elwood, Adam; Futyan, David; Haddad, Yacine; Hall, Geoffrey; Iles, Gregory; James, Thomas; Lane, Rebecca; Laner, Christian; Lyons, Louis; Magnan, Anne-Marie; Malik, Sarah; 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Wei, Hua; Wimpenny, Stephen; Yates, Brent; Branson, James G; Cerati, Giuseppe Benedetto; Cittolin, Sergio; Derdzinski, Mark; Gerosa, Raffaele; Holzner, André; Klein, Daniel; Krutelyov, Vyacheslav; Letts, James; Macneill, Ian; Olivito, Dominick; Padhi, Sanjay; Pieri, Marco; Sani, Matteo; Sharma, Vivek; Simon, Sean; Tadel, Matevz; Vartak, Adish; Wasserbaech, Steven; Welke, Charles; Wood, John; Würthwein, Frank; Yagil, Avraham; Zevi Della Porta, Giovanni; Amin, Nick; Bhandari, Rohan; Bradmiller-Feld, John; Campagnari, Claudio; Dishaw, Adam; Dutta, Valentina; Franco Sevilla, Manuel; George, Christopher; Golf, Frank; Gouskos, Loukas; Gran, Jason; Heller, Ryan; Incandela, Joe; Mullin, Sam Daniel; Ovcharova, Ana; Qu, Huilin; Richman, Jeffrey; Stuart, David; Suarez, Indara; Yoo, Jaehyeok; Anderson, Dustin; Bendavid, Joshua; Bornheim, Adolf; Bunn, Julian; Duarte, Javier; Lawhorn, Jay Mathew; Mott, Alexander; Newman, Harvey B; Pena, Cristian; Spiropulu, Maria; Vlimant, Jean-Roch; Xie, Si; Zhu, Ren-Yuan; Andrews, Michael Benjamin; Ferguson, Thomas; Paulini, Manfred; Russ, James; Sun, Menglei; Vogel, Helmut; Vorobiev, Igor; Weinberg, Marc; Cumalat, John Perry; Ford, William T; Jensen, Frank; Johnson, Andrew; Krohn, Michael; Leontsinis, Stefanos; Mulholland, Troy; Stenson, Kevin; Wagner, Stephen Robert; Alexander, James; Chaves, Jorge; Chu, Jennifer; Dittmer, Susan; Mcdermott, Kevin; Mirman, Nathan; Patterson, Juliet Ritchie; Rinkevicius, Aurelijus; Ryd, Anders; Skinnari, Louise; Soffi, Livia; Tan, Shao Min; Tao, Zhengcheng; Thom, Julia; Tucker, Jordan; Wittich, Peter; Zientek, Margaret; Winn, Dave; Abdullin, Salavat; Albrow, Michael; Apollinari, Giorgio; Apresyan, Artur; Banerjee, Sunanda; Bauerdick, Lothar AT; Beretvas, Andrew; Berryhill, Jeffrey; Bhat, Pushpalatha C; Bolla, Gino; Burkett, Kevin; Butler, Joel Nathan; Cheung, Harry; Chlebana, Frank; Cihangir, Selcuk; Cremonesi, Matteo; Elvira, Victor Daniel; Fisk, Ian; Freeman, Jim; Gottschalk, Erik; Gray, Lindsey; Green, Dan; Grünendahl, Stefan; Gutsche, Oliver; Hare, Daryl; Harris, Robert M; Hasegawa, Satoshi; Hirschauer, James; Hu, Zhen; Jayatilaka, Bodhitha; Jindariani, Sergo; Johnson, Marvin; Joshi, Umesh; Klima, Boaz; Kreis, Benjamin; Lammel, Stephan; Linacre, Jacob; Lincoln, Don; Lipton, Ron; Liu, Miaoyuan; Liu, Tiehui; Lopes De Sá, Rafael; Lykken, Joseph; Maeshima, Kaori; Magini, Nicolo; Marraffino, John Michael; Maruyama, Sho; Mason, David; McBride, Patricia; Merkel, Petra; Mrenna, Stephen; Nahn, Steve; O'Dell, Vivian; Pedro, Kevin; Prokofyev, Oleg; Rakness, Gregory; Ristori, Luciano; Sexton-Kennedy, Elizabeth; Soha, Aron; Spalding, William J; Spiegel, Leonard; Stoynev, Stoyan; Strait, James; Strobbe, Nadja; Taylor, Lucas; Tkaczyk, Slawek; Tran, Nhan Viet; Uplegger, Lorenzo; Vaandering, Eric Wayne; Vernieri, Caterina; Verzocchi, Marco; Vidal, Richard; Wang, Michael; Weber, Hannsjoerg Artur; Whitbeck, Andrew; Wu, Yujun; Acosta, Darin; Avery, Paul; Bortignon, Pierluigi; Bourilkov, Dimitri; Brinkerhoff, Andrew; Carnes, Andrew; 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Wu, Zhenbin; Zhang, Jingyu; Bilki, Burak; Clarida, Warren; Dilsiz, Kamuran; Durgut, Süleyman; Gandrajula, Reddy Pratap; Haytmyradov, Maksat; Khristenko, Viktor; Merlo, Jean-Pierre; Mermerkaya, Hamit; Mestvirishvili, Alexi; Moeller, Anthony; Nachtman, Jane; Ogul, Hasan; Onel, Yasar; Ozok, Ferhat; Penzo, Aldo; Snyder, Christina; Tiras, Emrah; Wetzel, James; Yi, Kai; Blumenfeld, Barry; Cocoros, Alice; Eminizer, Nicholas; Fehling, David; Feng, Lei; Gritsan, Andrei; Maksimovic, Petar; Roskes, Jeffrey; Sarica, Ulascan; Swartz, Morris; Xiao, Meng; You, Can; Al-bataineh, Ayman; Baringer, Philip; Bean, Alice; Boren, Samuel; Bowen, James; Castle, James; Forthomme, Laurent; Khalil, Sadia; Kropivnitskaya, Anna; Majumder, Devdatta; Mcbrayer, William; Murray, Michael; Sanders, Stephen; Stringer, Robert; Tapia Takaki, Daniel; Wang, Quan; Ivanov, Andrew; Kaadze, Ketino; Maravin, Yurii; Mohammadi, Abdollah; Saini, Lovedeep Kaur; Skhirtladze, Nikoloz; Toda, Sachiko; Rebassoo, Finn; Wright, Douglas; Anelli, Christopher; Baden, Drew; Baron, Owen; Belloni, Alberto; Calvert, Brian; Eno, Sarah Catherine; Ferraioli, Charles; Gomez, Jaime; Hadley, Nicholas John; Jabeen, Shabnam; Jeng, Geng-Yuan; Kellogg, Richard G; Kunkle, Joshua; Mignerey, Alice; Ricci-Tam, Francesca; Shin, Young Ho; Skuja, Andris; Tonjes, Marguerite; Tonwar, Suresh C; Abercrombie, Daniel; Allen, Brandon; Apyan, Aram; Azzolini, Virginia; Barbieri, Richard; Baty, Austin; Bi, Ran; Bierwagen, Katharina; Brandt, Stephanie; Busza, Wit; Cali, Ivan Amos; D'Alfonso, Mariarosaria; Demiragli, Zeynep; Gomez Ceballos, Guillelmo; Goncharov, Maxim; Hsu, Dylan; Iiyama, Yutaro; Innocenti, Gian Michele; Klute, Markus; Kovalskyi, Dmytro; Krajczar, Krisztian; Lai, Yue Shi; Lee, Yen-Jie; Levin, Andrew; Luckey, Paul David; Maier, Benedikt; Marini, Andrea Carlo; Mcginn, Christopher; Mironov, Camelia; Narayanan, Siddharth; Niu, Xinmei; Paus, Christoph; Roland, Christof; Roland, Gunther; Salfeld-Nebgen, Jakob; Stephans, George; Tatar, Kaya; Velicanu, Dragos; Wang, Jing; Wang, Ta-Wei; Wyslouch, Bolek; Benvenuti, Alberto; Chatterjee, Rajdeep Mohan; Evans, Andrew; Hansen, Peter; Kalafut, Sean; Kao, Shih-Chuan; Kubota, Yuichi; Lesko, Zachary; Mans, Jeremy; Nourbakhsh, Shervin; Ruckstuhl, Nicole; Rusack, Roger; Tambe, Norbert; Turkewitz, Jared; Acosta, John Gabriel; Oliveros, Sandra; Avdeeva, Ekaterina; Bloom, Kenneth; Claes, Daniel R; Fangmeier, Caleb; Gonzalez Suarez, Rebeca; Kamalieddin, Rami; Kravchenko, Ilya; Malta Rodrigues, Alan; Monroy, Jose; Siado, Joaquin Emilo; Snow, Gregory R; Stieger, Benjamin; Alyari, Maral; Dolen, James; Godshalk, Andrew; Harrington, Charles; Iashvili, Ia; Kaisen, Josh; Nguyen, Duong; Parker, Ashley; Rappoccio, Salvatore; Roozbahani, Bahareh; Alverson, George; Barberis, Emanuela; Hortiangtham, Apichart; Massironi, Andrea; Morse, David Michael; Nash, David; Orimoto, Toyoko; Teixeira De Lima, Rafael; Trocino, Daniele; Wang, Ren-Jie; Wood, Darien; Bhattacharya, Saptaparna; Charaf, Otman; Hahn, Kristan Allan; Mucia, Nicholas; Odell, Nathaniel; Pollack, Brian; Schmitt, Michael Henry; Sung, Kevin; Trovato, Marco; Velasco, Mayda; Dev, Nabarun; Hildreth, Michael; Hurtado Anampa, Kenyi; Jessop, Colin; Karmgard, Daniel John; Kellams, Nathan; Lannon, Kevin; Marinelli, Nancy; Meng, Fanbo; Mueller, Charles; Musienko, Yuri; Planer, Michael; Reinsvold, Allison; Ruchti, Randy; Rupprecht, Nathaniel; Smith, Geoffrey; Taroni, Silvia; Wayne, Mitchell; Wolf, Matthias; Woodard, Anna; Alimena, Juliette; Antonelli, Louis; Bylsma, Ben; Durkin, Lloyd Stanley; Flowers, Sean; Francis, Brian; Hart, Andrew; Hill, Christopher; Ji, Weifeng; Liu, Bingxuan; Luo, Wuming; Puigh, Darren; Winer, Brian L; Wulsin, Howard Wells; Cooperstein, Stephane; Driga, Olga; Elmer, Peter; Hardenbrook, Joshua; Hebda, Philip; Lange, David; Luo, Jingyu; Marlow, Daniel; Medvedeva, Tatiana; Mei, Kelvin; Ojalvo, Isabel; Olsen, James; Palmer, Christopher; Piroué, Pierre; Stickland, David; Svyatkovskiy, Alexey; Tully, Christopher; Malik, Sudhir; Barker, Anthony; Barnes, Virgil E; Folgueras, Santiago; Gutay, Laszlo; Jha, Manoj; Jones, Matthew; Jung, Andreas Werner; Khatiwada, Ajeeta; Miller, David Harry; Neumeister, Norbert; Schulte, Jan-Frederik; Shi, Xin; Sun, Jian; Wang, Fuqiang; Xie, Wei; Parashar, Neeti; Stupak, John; Adair, Antony; Akgun, Bora; Chen, Zhenyu; Ecklund, Karl Matthew; Geurts, Frank JM; Guilbaud, Maxime; Li, Wei; Michlin, Benjamin; Northup, Michael; Padley, Brian Paul; Roberts, Jay; Rorie, Jamal; Tu, Zhoudunming; Zabel, James; Betchart, Burton; Bodek, Arie; de Barbaro, Pawel; Demina, Regina; Duh, Yi-ting; Ferbel, Thomas; Galanti, Mario; Garcia-Bellido, Aran; Han, Jiyeon; Hindrichs, Otto; Khukhunaishvili, Aleko; Lo, Kin Ho; Tan, Ping; Verzetti, Mauro; Agapitos, Antonis; Chou, John Paul; Gershtein, Yuri; Gómez Espinosa, Tirso Alejandro; Halkiadakis, Eva; Heindl, Maximilian; Hughes, Elliot; Kaplan, Steven; Kunnawalkam Elayavalli, Raghav; Kyriacou, Savvas; Lath, Amitabh; Montalvo, Roy; Nash, Kevin; Osherson, Marc; Saka, Halil; Salur, Sevil; Schnetzer, Steve; 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2017-08-22

A measurement of the top quark mass ($M_{\\mathrm{ t }}$) in the dileptonic $\\mathrm{ t \\bar{t} }$ decay channel is performed using data from proton-proton collisions at a center-of-mass energy of 8 TeV. The data was recorded by the CMS experiment at the LHC and corresponding to an integrated luminosity of 19.7 $\\pm$ 0.5 fb$^{-1}$. Events are selected with two oppositely charged leptons ($\\ell=$ e, $\\mu$) and two jets identified as originating from b quarks. The analysis is based on three kinematic observables whose distributions are sensitive to the value of $M_{\\mathrm{ t }}$. An invariant mass observable, $M_{\\mathrm{ b }\\ell}$, and a 'stransverse mass' observable, $M_{\\mathrm{T}2}$, are employed in a simultaneous fit to determine the value of $M_{\\mathrm{ t }}$ and an overall jet energy scale factor (JSF). A complementary approach is used to construct an invariant mass observable, $M_{\\mathrm{ b }\\ell\

Inhibition by sigma receptor ligand, MS-377, of N-methyl- D-aspartate-induced currents in dopamine neurons of the rat ventral tegmental area.

Science.gov (United States)

Yamazaki, Yuu; Ishioka, Miwa; Matsubayashi, Hiroaki; Amano, Taku; Sasa, Masashi

2002-04-01

MS-377 [( R)-(+)-1-(4-chlorophenyl)-3-[4-(2-methoxyethyl) piperazin-1-yl]methyl-2-pyrrolidinone L-tartrate] is a novel anti-psychotic drug candidate with high affinity for sigma receptors but devoid of binding affinity for PCP binding site of NMDA receptor/ion channel complex. The effects of MS-377 on NMDA receptor and/or its ion channel complex were examined to elucidate the antipsychotic properties of MS-377. We examined the effect of MS-377 on NMDA ( N-methyl- D-aspartate)-induced current in acutely dissociated dopamine neurons of rat ventral tegmental area (VTA) using patch clamp whole cell recording. MS-377 applied in a bath inhibited the peak current evoked by NMDA applied via the U-tube method for 2 s in a concentration-dependent manner. Other sigma receptor ligands, BD-1063 (1-[2-(3,4-dichlorophenyl)ethyl]-4-methylpiperazine), NE-100 ( N, N-dipropyl-2-[4-methoxy-3-(2-phenylenoxy)-phenyl]-ethylamine monohydrochloride) and haloperidol also inhibited NMDA-induced current in a concentration-dependent manner. Interestingly, concomitant application of MS-377 with BD-1063, NE-100 or haloperidol at concentrations that had no effects on NMDA-induced current, potentiated the MS-377-induced inhibition. The results suggest that MS-377, as well as other sigma receptor ligands, indirectly acts on the sigma receptor to inhibit glutaminergic transmission mediated by NMDA receptor/ion channel complex in VTA dopamine neurons, thereby inhibiting dopamine release in target VTA areas.

Access Control in IoT/M2M - Cloud Platform

DEFF Research Database (Denmark)

Anggorojati, Bayu

Billions of devices are connected to the Internet nowadays, and the number will continue to grow in the future thanks to the advances in the electronics and telecommunication technology developments. Its application in broad aspects of human’s life brings a lot of benefits by improving productivity...... and quality of life. This paradigm, which is often called Internet of Things (IoT) or Machine-to-Machine (M2M), will provide an unprecedented opportunity to create applications and services that go far beyond the mere purpose of each participant. Many studies on the both technical and social aspects of Io......T have shown that the concern about the security and privacy play a huge role for the mass adoption of the IoT/M2M as cloud services. Among the important topics within the security and privacy, the access control is an important mechanism, which essentially manages how the important assets or resource...

Identification of the GST-T1 and GST-M1 null genotypes using high resolution melting analysis.

Science.gov (United States)

Drobná, Zuzana; Del Razo, Luz Maria; Garcia-Vargas, Gonzalo; Sánchez-Ramírez, Blanca; González-Horta, Carmen; Ballinas-Casarrubias, Lourdes; Loomis, Dana; Stýblo, Miroslav

2012-01-13

Glutathione S-transferases, including GST-T1 and GST-M1, are known to be involved in the phase II detoxification pathways for xenobiotics as well as in the metabolism of endogenous compounds. Polymorphisms in these genes have been linked to an increased susceptibility to carcinogenesis and associated with risk factors that predispose to certain inflammatory diseases. In addition, GST-T1 and GST-M1 null genotypes have been shown to be responsible for interindividual variations in the metabolism of arsenic, a known human carcinogen. To assess the specific GST genotypes in the Mexican population chronically exposed to arsenic, we have developed a multiplex High Resolution Melting PCR (HRM-PCR) analysis using a LightCycler480 instrument. This method is based on analysis of the PCR product melting curve that discriminates PCR products according to their lengths and base sequences. Three pairs of primers that specifically recognize GST-T1, GST-M1, and β-globin, an internal control, to produce amplicons of different length were designed and combined with LightCycler480 High Resolution Melting Master Mix containing ResoLight, a completely saturating DNA dye. Data collected from melting curve analysis were evaluated using LightCycler480 software to determine specific melting temperatures of individual melting curves representing target genes. Using this newly developed multiplex HRM-PCR analysis, we evaluated GST-T1 and GST-M1 genotypes in 504 DNA samples isolated from the blood of individuals residing in Zimapan, Lagunera, and Chihuahua regions in Mexico. We found that the Zimapan and Lagunera populations have similar GST-T1 and GST-M1 genotype frequencies which differ from those of the Chihuahua population. In addition, 14 individuals have been identified as carriers of the double null genotype, i.e., null genotypes in both GST-T1 and GST-M1 genes. Although this procedure does not distinguish between biallelic (+/+) and monoallelic (+/-) genotypes, it can be used in an

Gri Suyun Arıtımı ve Yeniden Kullanımı

OpenAIRE

ÜSTÜN, Gökhan; TIRPANCI, Ayşenur

2015-01-01

Çalışmanın amacı, gri su arıtımının ve yeniden kullanılması konusunun incelenmesidir. Bu amaç için daha önce yapılmış literatür çalışmaları araştırılıp, yorumlanmıştır. Çalışmanın ilk aşamasında gri suyun tanımlanması ile fiziksel, kimyasal ve biyolojik karakteristiği açıklanmıştır. İkinci kısmında, gri suyun arıtım yöntemleri ve yeniden kullanımı incelenmiştir. Üçüncü kısımda gri suların arıtımında kullanılan teknolojiler tek tek açıklanmıştır. Son olarak gri su arıtımı ve yeniden kullanımı ...

Evaluation of T-lymphocyte populations in humans exposed to action of beryllium compounds

Energy Technology Data Exchange (ETDEWEB)

Ermakova, N G

1977-10-01

Earlier studies in vitro on beryllosis and cellular immunity, and knowledge of the role of T-cells in development of cellular immunity suggested usefulness of a quantitative determination of T-cell populations in beryllosis. Assay was based on the test for spontaneous rosette formation in beryllosis patients and in healthy workers who come in contact with Be compounds (Jondal, modified by M. A. Stenina) and on the leucocyte migration inhibition reaction (Soborg and Bendixen). A large range of changes were revealed in population size and in adhesion qualities of T-lymphocytes in the patients and in those exposed to Be. The test for spontaneous rosette formation was found to be sufficiently indicative for use in study of beryllosis pathogenesis and to correlate with the state of activity of the disease.

A sub-population of circulating porcine gammadelta T cells can act as professional antigen presenting cells.

Science.gov (United States)

Takamatsu, H-H; Denyer, M S; Wileman, T E

2002-09-10

A sub-population of circulating porcine gammadelta T cells express cell surface antigens associated with antigen presenting cells (APCs), and are able to take up soluble antigen very effectively. Functional antigen presentation by gammadelta T cells to memory helper T cells was studied by inbred pig lymphocytes immunised with ovalbumin (OVA). After removing all conventional APCs from the peripheral blood of immunised pigs, the remaining lymphocytes still proliferated when stimulated with OVA. When gammadelta T cells were further depleted, OVA specific proliferation was abolished, but reconstitution with gammadelta T cells restored proliferation. The proliferation was blocked by monoclonal antibodies (mAb) against MHC class II or CD4, and by pre-treatment of gammadelta T cells with chloroquine. These results indicate that a sub-population of circulating porcine gammadelta T cells act as APCs and present antigen via MHC class II.

Genetic polymorphisms of glutathione-s-transferase M1 and T1 genes with risk of diabetic retinopathy in Iranian population

Directory of Open Access Journals (Sweden)

Elham Moasser

2014-05-01

Full Text Available Objective(s:To the best of our knowledge, this is the first report on the contributions of GST genetic variants to the risk of diabetic retinopathy in an Iranian population. Therefore, the objective of this study was to determine whether sequence variation in glutathione S-transferase gene (GSTM1 and GSTT1 is associated with development of diabetic retinopathy in type 2 diabetes mellitus (T2DM Iranian patients. Materials and Methods: A total of 605 subjects were investigated in this case-control study; Study groups consisted of 201 patients with diabetic retinopathy (DR, 203 subjects with no clinically significant signs of DR and a group of 201 cases of healthy volunteers with no clinical evidence of diabetes mellitus or any other diseases. The GSTM1 and GSTT1 were genotyped by multiplex-polymerase chain reaction (multiplex-PCR analysis in all 404 T2DM patients and 201 healthy individuals served as control. Results: Increased odds ratio showed that GSTM1-null genotype had a moderately higher occurrence in T2DM patients (OR=1.43, 95% CI=1.01–2.04; P=0.03 than in healthy individuals. However, the frequency of GSTT1 genotype (OR=1.41; 95% CI=0.92-2.18; P=0.09 was not significantly different comparing both groups. Although, regression analysis in T2DM patients showed that GSTM1 and GSTT1 genotypes are not associated with T2DM retinopathy development. Conclusion: Our findings suggest that GSTM1 and GSTT1 genotypes might not be involved in the pathogenesis of type 2 diabetes mellitus retinopathy in the Southern Iranian population. However, further investigations are needed to confirm these results in other larger populations.

The T.M. Calorimeter

International Nuclear Information System (INIS)

Mas, P.; Goer, J. de

1970-01-01

The T.M. calorimeter is the isothermal type. It consists only of a sample of graphite and a jacket of stainless steel filled with nitrogen. The chromel-alumel thermocouples which measure the temperature difference between the sample and the jacket also serve to suspend the sample. The jacket is kept at a constant temperature: i.e. that of the water in the swimming pool

Li collection experiments on T-11M and T-10 in framework of Li closed loop concept

International Nuclear Information System (INIS)

Mirnov, Sergey V.; Alekseev, Andrey G.; Belov, Alexandr M.; Djigailo, Nadejda T.; Kostina, Anastasiya N.; Lazarev, Vladimir B.; Lyublinski, Igor E.; Nesterenko, Vladislav M.; Vertkov, Aleksei V.; Vershkov, Vladimir A.

2012-01-01

Highlights: ► We investigated the Li collection by different type of limiters intersecting the scrape-of-layer (SOL) of T-10 and T-11M tokamaks. ► The analysis of the sample-witnesses located on T-11M limiters showed, that 60 ± 20% of the lithium injected during plasma operating of T-11M had been collected by limiters. ► We believe that is the real opportunity of the tokamak plasma facing components (PFC) development on the basis of liquid lithium circulation. - Abstract: The concept of a steady state tokamak with plasma facing components (PFC) on the basis of liquid lithium circulation demands the decision of three tasks: lithium injection to the plasma, lithium ions collection before their deposition on the vacuum vessel and lithium returning to the injection zone. Main subject of paper is the investigations of Li collection by different types of limiters intersected the scrape-of-layer (SOL) in T-10 and T-11M tokamaks. For finding solution for this problem in T-11M and T-10, experiments have been applied with Li-, C-rail limiters and ring SS R-limiter-collector (T-11M). The efficiency of Li collection by limiters in T-11M and T-10 tokamaks was investigated by post mortem sample–witness analysis and (T-11M) by the use of the mobile graphite probe (limiter) as a recombination target in the stream of lithium ions. The characteristic depth of lithium penetration in the SOL area of T-11M is about 2 cm and 4 cm in SOL of T-10. The quantitative analysis of the sample–witnesses located on T-11M limiters showed that 60 ± 20% of the lithium injected during plasma operating of T-11M had been collected by limiters. It confirms an opportunity of the lithium ions collection by limiters in tokamak SOL.

34 CFR 377.1 - What is the Demonstration Projects to Increase Client Choice Program?

Science.gov (United States)

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What is the Demonstration Projects to Increase Client... PROJECTS TO INCREASE CLIENT CHOICE PROGRAM General § 377.1 What is the Demonstration Projects to Increase Client Choice Program? The Demonstration Projects to Increase Client Choice Program is designed to...

Predictions for m{sub t} and M{sub W} in minimal supersymmetric models

Energy Technology Data Exchange (ETDEWEB)

Buchmueller, O. [Imperial College, London (United Kingdom). High Energy Physics Group; Cavanaugh, R. [Fermi National Accelerator Lab., Batavia, IL (United States); Illinois Univ., Chicago, IL (United States). Dept. of Physics; Roeck, A. de [European Lab. for Particle Physics (CERN), Geneva (Switzerland); Universitaire Instelling Antwerpen, Wilrijk (Belgium); Ellis, J.R. [European Lab. for Particle Physics (CERN), Geneva (Switzerland); Flaecher, H. [Rochester Univ., NY (United States). Dept. of Physics and Astronomy; Heinemeyer, S. [Instituto de Fisica de Cantabria, Santander (Spain); Isidori, G. [INFN, Laboratori Nazionali di Frascati (Italy); Technische Univ. Muenchen (Germany). Inst. for Advanced Study; Olive, K.A. [Minnesota Univ., Minnesota, MN (United States). William I. Fine Theoretical Physics Institute; Ronga, F.J. [ETH Zuerich (Switzerland). Institute for Particle Physics; Weiglein, G. [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)

2009-12-15

Using a frequentist analysis of experimental constraints within two versions of the minimal supersymmetric extension of the Standard Model, we derive the predictions for the top quark mass, m{sub t}, and the W boson mass, m{sub W}. We find that the supersymmetric predictions for both m{sub t} and m{sub W}, obtained by incorporating all the relevant experimental information and state-of-the-art theoretical predictions, are highly compatible with the experimental values with small remaining uncertainties, yielding an improvement compared to the case of the Standard Model. (orig.)

Cellular size as a means of tracking mTOR activity and cell fate of CD4+ T cells upon antigen recognition.

Directory of Open Access Journals (Sweden)

Kristen N Pollizzi

Full Text Available mTOR is a central integrator of metabolic and immunological stimuli, dictating immune cell activation, proliferation and differentiation. In this study, we demonstrate that within a clonal population of activated T cells, there exist both mTORhi and mTORlo cells exhibiting highly divergent metabolic and immunologic functions. By taking advantage of the role of mTOR activation in controlling cellular size, we demonstrate that upon antigen recognition, mTORhi CD4+ T cells are destined to become highly glycolytic effector cells. Conversely, mTORlo T cells preferentially develop into long-lived cells that express high levels of Bcl-2, CD25, and CD62L. Furthermore, mTORlo T cells have a greater propensity to differentiate into suppressive Foxp3+ T regulatory cells, and this paradigm was also observed in human CD4+ T cells. Overall, these studies provide the opportunity to track the development of effector and memory T cells from naïve precursors, as well as facilitate the interrogation of immunologic and metabolic programs that inform these fates.

ArtinM Mediates Murine T Cell Activation and Induces Cell Death in Jurkat Human Leukemic T Cells

Science.gov (United States)

Oliveira-Brito, Patrícia Kellen Martins; Gonçalves, Thiago Eleutério; Vendruscolo, Patrícia Edivânia; Roque-Barreira, Maria Cristina

2017-01-01

The recognition of cell surface glycans by lectins may be critical for the innate and adaptive immune responses. ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing TLR2 N-glycans and induces Th1 immunity. We recently demonstrated that ArtinM stimulated CD4+ T cells to produce proinflammatory cytokines. Here, we further studied the effects of ArtinM on adaptive immune cells. We showed that ArtinM activates murine CD4+ and CD8+ T cells, augmenting their positivity for CD25, CD69, and CD95 and showed higher interleukin (IL)-2 and interferon (IFN)-γ production. The CD4+ T cells exhibited increased T-bet expression in response to ArtinM, and IL-2 production by CD4+ and CD8+ T cells depended on the recognition of CD3εγ-chain glycans by ArtinM. The ArtinM effect on aberrantly-glycosylated neoplastic lymphocytes was studied in Jurkat T cells, in which ArtinM induced IL-2, IFN-γ, and IL-1β production, but decreased cell viability and growth. A higher frequency of AnnexinV- and propidium iodide-stained cells demonstrated the induction of Jurkat T cells apoptosis by ArtinM, and this apoptotic response was reduced by caspases and protein tyrosine kinase inhibitors. The ArtinM effects on murine T cells corroborated with the immunomodulatory property of lectin, whereas the promotion of Jurkat T cells apoptosis may reflect a potential applicability of ArtinM in novel strategies for treating lymphocytic leukemia. PMID:28665310

Inflammatory mediator bradykinin increases population of sensory neurons expressing functional T-type Ca(2+) channels.

Science.gov (United States)

Huang, Dongyang; Liang, Ce; Zhang, Fan; Men, Hongchao; Du, Xiaona; Gamper, Nikita; Zhang, Hailin

2016-04-29

T-type Ca(2+) channels are important regulators of peripheral sensory neuron excitability. Accordingly, T-type Ca(2+) currents are often increased in various pathological pain conditions, such as inflammation or nerve injury. Here we investigated effects of inflammation on functional expression of T-type Ca(2+) channels in small-diameter cultured dorsal root ganglion (DRG) neurons. We found that overnight treatment of DRG cultures with a cocktail of inflammatory mediators bradykinin (BK), adenosine triphosphate (ATP), norepinephrine (NE) and prostaglandin E2 (PGE2) strongly increased the population size of the small-diameter neurons displaying low-voltage activated (LVA, T-type) Ca(2+) currents while having no effect on the peak LVA current amplitude. When applied individually, BK and ATP also increased the population size of LVA-positive neurons while NE and PGE2 had no effect. The PLC inhibitor U-73122 and B2 receptor antagonist, Hoe-140, both abolished the increase of the population of LVA-positive DRG neurons. Inflammatory treatment did not affect CaV3.2 mRNA or protein levels in DRG cultures. Furthermore, an ubiquitination inhibitor, MG132, did not increase the population of LVA-positive neurons. Our data suggest that inflammatory mediators BK and ATP increase the abundance of LVA-positive DRG neurons in total neuronal population by stimulating the recruitment of a 'reserve pool' of CaV3.2 channels, particularly in neurons that do not display measurable LVA currents under control conditions. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Plantes médicinales utilisées par les populations Bassa de la région ...

African Journals Online (AJOL)

Une enquête ethnobotanique portant sur un échantillon de 90 ménages a été conduite en juin 2010 dans trois villages de la région de Douala, au Cameroun, afin de recenser les plantes médicinales utilisées par les populations rurales. Elle montre que 48 espèces relevant de 44 genres et de 26 familles sont utilisées dans ...

Association between RAS Gene Polymorphisms (ACE I/D, AGT M235T and Henoch-Schönlein Purpura in a Turkish Population

Directory of Open Access Journals (Sweden)

Sinem Nalbantoglu

2013-01-01

Full Text Available Henoch-Schönlein purpura (HSP is a small-vessel vasculitis of autoimmune hypersensitivity, and renin-angiotensin system (RAS regulates vascular homeostasis and inflammation with activation of cytokine release. Thus, we aimed to investigate the association between HSP and ACE I/D and AGT M235T polymorphisms. Genotyping was determined by allele specific PCR and PCR-RFLP. We obtained a significant difference in genotype distribution (p = 0.003 and allele frequencies (p 0.05 and allele frequencies (p > 0.05 of the AGT M235T polymorphism. Risk assessment showed significant risk for HSP in the subjects both with the ID + DD genotype (p = 0.019, OR: 2.288, 95% CI: 1.136–4.609 and D allele (OR: D vs. I: 2.0528, 95% CI: 1.3632–3.0912, p = 0.001 while no significant risk was obtained for HSP in the subjects both with the MT + TT genotype (p = 0.312, OR: 1.3905, 95% CI: 0.7326–2.6391 and T allele (OR: T vs. M: 1.065, 95% CI: 0.729–1.557, p = 0.743. Furthermore, when patients were stratified by the presence of certain systemic complications of HSP, no significant association was detected with ACE I/D, and AGT M235T polymorphisms. Our findings suggest that ACE I/D polymorphism is significantly associated with HSP susceptibility.

From WSN towards WoT: Open API Scheme Based on oneM2M Platforms

Science.gov (United States)

Kim, Jaeho; Choi, Sung-Chan; Ahn, Il-Yeup; Sung, Nak-Myoung; Yun, Jaeseok

2016-01-01

Conventional computing systems have been able to be integrated into daily objects and connected to each other due to advances in computing and network technologies, such as wireless sensor networks (WSNs), forming a global network infrastructure, called the Internet of Things (IoT). To support the interconnection and interoperability between heterogeneous IoT systems, the availability of standardized, open application programming interfaces (APIs) is one of the key features of common software platforms for IoT devices, gateways, and servers. In this paper, we present a standardized way of extending previously-existing WSNs towards IoT systems, building the world of the Web of Things (WoT). Based on the oneM2M software platforms developed in the previous project, we introduce a well-designed open API scheme and device-specific thing adaptation software (TAS) enabling WSN elements, such as a wireless sensor node, to be accessed in a standardized way on a global scale. Three pilot services are implemented (i.e., a WiFi-enabled smart flowerpot, voice-based control for ZigBee-connected home appliances, and WiFi-connected AR.Drone control) to demonstrate the practical usability of the open API scheme and TAS modules. Full details on the method of integrating WSN elements into three example systems are described at the programming code level, which is expected to help future researchers in integrating their WSN systems in IoT platforms, such as oneM2M. We hope that the flexibly-deployable, easily-reusable common open API scheme and TAS-based integration method working with the oneM2M platforms will help the conventional WSNs in diverse industries evolve into the emerging WoT solutions. PMID:27782058

From WSN towards WoT: Open API Scheme Based on oneM2M Platforms

Directory of Open Access Journals (Sweden)

Jaeho Kim

2016-10-01

Full Text Available Conventional computing systems have been able to be integrated into daily objects and connected to each other due to advances in computing and network technologies, such as wireless sensor networks (WSNs, forming a global network infrastructure, called the Internet of Things (IoT. To support the interconnection and interoperability between heterogeneous IoT systems, the availability of standardized, open application programming interfaces (APIs is one of the key features of common software platforms for IoT devices, gateways, and servers. In this paper, we present a standardized way of extending previously-existing WSNs towards IoT systems, building the world of the Web of Things (WoT. Based on the oneM2M software platforms developed in the previous project, we introduce a well-designed open API scheme and device-specific thing adaptation software (TAS enabling WSN elements, such as a wireless sensor node, to be accessed in a standardized way on a global scale. Three pilot services are implemented (i.e., a WiFi-enabled smart flowerpot, voice-based control for ZigBee-connected home appliances, and WiFi-connected AR.Drone control to demonstrate the practical usability of the open API scheme and TAS modules. Full details on the method of integrating WSN elements into three example systems are described at the programming code level, which is expected to help future researchers in integrating their WSN systems in IoT platforms, such as oneM2M. We hope that the flexibly-deployable, easily-reusable common open API scheme and TAS-based integration method working with the oneM2M platforms will help the conventional WSNs in diverse industries evolve into the emerging WoT solutions.

From WSN towards WoT: Open API Scheme Based on oneM2M Platforms.

Science.gov (United States)

Kim, Jaeho; Choi, Sung-Chan; Ahn, Il-Yeup; Sung, Nak-Myoung; Yun, Jaeseok

2016-10-06

Conventional computing systems have been able to be integrated into daily objects and connected to each other due to advances in computing and network technologies, such as wireless sensor networks (WSNs), forming a global network infrastructure, called the Internet of Things (IoT). To support the interconnection and interoperability between heterogeneous IoT systems, the availability of standardized, open application programming interfaces (APIs) is one of the key features of common software platforms for IoT devices, gateways, and servers. In this paper, we present a standardized way of extending previously-existing WSNs towards IoT systems, building the world of the Web of Things (WoT). Based on the oneM2M software platforms developed in the previous project, we introduce a well-designed open API scheme and device-specific thing adaptation software (TAS) enabling WSN elements, such as a wireless sensor node, to be accessed in a standardized way on a global scale. Three pilot services are implemented (i.e., a WiFi-enabled smart flowerpot, voice-based control for ZigBee-connected home appliances, and WiFi-connected AR.Drone control) to demonstrate the practical usability of the open API scheme and TAS modules. Full details on the method of integrating WSN elements into three example systems are described at the programming code level, which is expected to help future researchers in integrating their WSN systems in IoT platforms, such as oneM2M. We hope that the flexibly-deployable, easily-reusable common open API scheme and TAS-based integration method working with the oneM2M platforms will help the conventional WSNs in diverse industries evolve into the emerging WoT solutions.

The potential of Internet of m-health Things "m-IoT" for non-invasive glucose level sensing.

Science.gov (United States)

Istepanian, R S H; Hu, S; Philip, N Y; Sungoor, A

2011-01-01

An amalgamated concept of Internet of m-health Things (m-IoT) has been introduced recently and defined as a new concept that matches the functionalities of m-health and IoT for a new and innovative future (4G health) applications. It is well know that diabetes is a major chronic disease problem worldwide with major economic and social impact. To-date there have not been any studies that address the potential of m-IoT for non-invasive glucose level sensing with advanced opto-physiological assessment technique and diabetes management. In this paper we address the potential benefits of using m-IoT in non-invasive glucose level sensing and the potential m-IoT based architecture for diabetes management. We expect to achieve intelligent identification and management in a heterogeneous connectivity environment from the mobile healthcare perspective. Furthermore this technology will enable new communication connectivity routes between mobile patients and care services through innovative IP based networking architectures.

Identifying Multiple Populations in M71 using CN

Science.gov (United States)

Gerber, Jeffrey M.; Friel, Eileen D.; Vesperini, Enrico

2018-01-01

It is now well established that globular clusters (GCs) host multiple stellar populations characterized by differences in several light elements. While these populations have been found in nearly all GCs, we still lack an entirely successful model to explain their formation. A key constraint to these models is the detailed pattern of light element abundances seen among the populations; different techniques for identifying these populations probe different elements and do not always yield the same results. We study a large sample of stars in the GC M71 for light elements C and N, using the CN and CH band strength to identify multiple populations. Our measurements come from low-resolution spectroscopy obtained with the WIYN-3.5m telescope for ~150 stars from the tip of the red-giant branch down to the main-sequence turn-off. The large number of stars and broad spatial coverage of our sample (out to ~3.5 half-light radii) allows us to carry out a comprehensive characterization of the multiple populations in M71. We use a combination of the various spectroscopic and photometric indicators to draw a more complete picture of the properties of the populations and to investigate the consistency of classifications using different techniques.

Detection of T790M, the acquired resistance EGFR mutation, by tumor biopsy versus noninvasive blood-based analyses

Science.gov (United States)

Sundaresan, Tilak K.; Sequist, Lecia V.; Heymach, John V.; Riely, Gregory J.; Jänne, Pasi A.; Koch, Walter H.; Sullivan, James P.; Fox, Douglas B.; Maher, Robert; Muzikansky, Alona; Webb, Andrew; Tran, Hai T.; Giri, Uma; Fleisher, Martin; Yu, Helena A.; Wei, Wen; Johnson, Bruce E.; Barber, Thomas A.; Walsh, John R.; Engelman, Jeffrey A.; Stott, Shannon L.; Kapur, Ravi; Maheswaran, Shyamala; Toner, Mehmet

2015-01-01

Purpose The T790M gatekeeper mutation in the Epidermal Growth Factor Receptor (EGFR) is acquired by some EGFR-mutant non-small cell lung cancers (NSCLC) as they become resistant to selective tyrosine kinase inhibitors (TKIs). As third generation EGFR TKIs that overcome T790M-associated resistance become available, noninvasive approaches to T790M detection will become critical to guide management. Experimental Design As part of a multi-institutional Stand-Up-To-Cancer collaboration, we performed an exploratory analysis of 40 patients with EGFR-mutant tumors progressing on EGFR TKI therapy. We compared the T790M genotype from tumor biopsies with analysis of simultaneously collected circulating tumor cells (CTC) and circulating tumor DNA (ctDNA). Results T790M genotypes were successfully obtained in 30 (75%) tumor biopsies, 28 (70%) CTC samples and 32 (80%) ctDNA samples. The resistance-associated mutation was detected in 47–50% of patients using each of the genotyping assays, with concordance among them ranging from 57–74%. While CTC- and ctDNA-based genotyping were each unsuccessful in 20–30% of cases, the two assays together enabled genotyping in all patients with an available blood sample, and they identified the T790M mutation in 14 (35%) patients in whom the concurrent biopsy was negative or indeterminate. Conclusion Discordant genotypes between tumor biopsy and blood-based analyses may result from technological differences, as well as sampling different tumor cell populations. The use of complementary approaches may provide the most complete assessment of each patient’s cancer, which should be validated in predicting response to T790M-targeted inhibitors. PMID:26446944

Insulīna rezistence PCOS pacientēm un tās saistība ar citiem riska faktoriem (kardiovaskulāro slimību, cukura diabēts)

OpenAIRE

Gutņikovs, Oļegs

2014-01-01

Darba pamatojums: PCOS ir viena no biežākajām endokrinopātijām sievietēm reproduktīvajā vecumā. Pēc literatūras datiem, pacientēm ar PCOS pieaug KVS un cukura diabēta attīstības risks. Tāpēc šajā pētījumā tiek analizēts, vai šie dati attiecināmi arī uz Latvijas populāciju. Pētījuma mērķis: salīdzināt KVS attīstības risku PCOS pacientēm ar vispārējo populāciju Latvijas teritorijā. Materiāli un metodes: Retrospektīva pētījuma ietvaros tika analizētas slimības vēstures PCOS pacientēm (E28...

Measuring high-sensitivity cardiac troponin T blood concentration in population surveys.

OpenAIRE

Lazzarino, AI; Mindell, JS

2017-01-01

Introduction The blood test for high-sensitivity cardiac troponin T (HS-CTnT) has been proposed as a marker of cardiovascular risk in the general population, as it is associated with subsequent incidence of cardiovascular events and mortality. We aimed at evaluating the feasibility of HS-CTnT testing within large nationally-representative population surveys in which blood samples are collected during household visits, shipped using the standard civil postal service, and then frozen for subseq...

Measuring high-sensitivity cardiac troponin T blood concentration in population surveys

OpenAIRE

Lazzarino, A. I.; Mindell, J. S.

2017-01-01

INTRODUCTION: The blood test for high-sensitivity cardiac troponin T (HS-CTnT) has been proposed as a marker of cardiovascular risk in the general population, as it is associated with subsequent incidence of cardiovascular events and mortality. We aimed at evaluating the feasibility of HS-CTnT testing within large nationally-representative population surveys in which blood samples are collected during household visits, shipped using the standard civil postal service, and then frozen for subse...

Retracted: Association of ACE I/D gene polymorphism with T2DN susceptibility and the risk of T2DM developing into T2DN in a Caucasian population.

Science.gov (United States)

Liu, Guohui; Zhou, Tian-Biao; Jiang, Zongpei; Zheng, Dongwen

2015-03-01

The association of the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) gene polymorphism with type-2 diabetic nephropathy (T2DN) susceptibility and the risk of type-2 diabetes mellitus (T2DM) developing into T2DN in Caucasian populations is still controversial. A meta-analysis was performed to evaluate the association of ACE I/D gene polymorphism with T2DN susceptibility and the risk of T2DM developing into T2DN in Caucasian populations. A predefined literature search and selection of eligible relevant studies were performed to collect data from electronic databases. Sixteen articles were identified for the analysis of the association of ACE I/D gene polymorphism with T2DN susceptibility and the risk of T2DM developing into T2DN in Caucasian populations. ACE I/D gene polymorphism was not associated with T2DN susceptibility and the risk of patients with T2DM developing T2DN in Caucasian populations. Sensitivity analysis according to sample size of case (ACE I/D gene polymorphism was not associated with T2DN susceptibility and the risk of patients with T2DM developing T2DN in Caucasian populations. However, more studies should be performed in the future. © The Author(s) 2014.

sup(113m)In E.D.T.M.P

International Nuclear Information System (INIS)

Nowotny, G.; Fraga de Suarez, A.H.; Mitta, A.E.A.

1978-05-01

A radiopharmaceutical prepared from ethylendiaminotetrametilenphosphoric acid (E.T.M.P.) is described, which can be labelled with 113 In for its use in bone scintillography. This compound has been tested satisfactorily in animals. Chemical controls for labelling are discussed as well as the biological ones for distribution and toxicity. A radiochemical yield of 95% or higher was obtained and after two hours of administration nearly 30% of dose can be localized is skeleton. (author) [es

Lavenergihuset tæt på målet!

DEFF Research Database (Denmark)

Rode, Carsten

2012-01-01

Lavenergihuset i Sisimiut blev indviet i april 2005. Det var projekteret til at have et årligt energiforbrug til opvarmning på 80 kWh/m2. Men gennem de første 4½ år af husets levetid har forbruget vist sig at ligge på ca. 140 kWh/m2 – altså ca. 75% mere end planlagt. I vinteren 2009/10 blev der dog...... isoleringsevnen er blevet bedre. Alt dette har gjort, at energiforbruget nu er nede på ca. 95 kWh/m2 målt fra december 2009 til november 2010. Indendørs har temperaturen været på ca. 23°C, mod de 21°C huset var planlagt til at have, og det teoretiske energiforbrug ved den højere indendørs temperatur skulle så...... muligt at redegøre for det sidste, der mangler i at husets ydeevne når målet. Umiddelbart betragtet har det givet en række udfordringer at følge huset og dets energiforbrug. Den tætte opfølgning har dog givet nogle unikke erfaringer, som de involverede og branchen kan tage ved lære af. I foredraget gives...

26 CFR 1.103(n)-6T - Determinations of population (temporary).

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Determinations of population (temporary). 1.103....103(n)-6T Determinations of population (temporary). Q-1: What is the proper method for determining population? A-1: All determinations of population must be made with respect to any calendar year on the basis...

Saksa krambiteater - levinud müüt / Laur Kaunissaare

Index Scriptorium Estoniae

Kaunissaare, Laur, 1982-

2004-01-01

Saksa teatrites nähtud lavastustest - R. Wilsoni lavastus "Leonce ja Lena" Berliner Ensemble'is, T. Ostermeieri "Nukumaja" Schaubühnes, Berliner Ensemble'i proovilaval mängitav monodraama "Artaud meenutab Adolf Hitlerit ja Romanisches Cafed" (esitab M. Wuttke), M. Thalheimeri lavastus "Emilia Galotti" Deutsches Theateris

A preliminary study measuring the number of T-cell receptor-rearrangement excision circles (TRECs) in peripheral blood T-cell populations of A-bomb survivors and control populations

International Nuclear Information System (INIS)

Kubo, Yoshiko; Yamaoka, Mika; Kusunoki, Yoichiro

2006-01-01

More than a half century after damage of the immune systems by the radiation from A-bomb, we can still observe significant decreases in the percentages of naieve CD4 and CD8 T cells among the survivors. To investigate whether the observed decreases in the naieve T-cell populations may have resulted from reduction in thymic T-cell production ability of survivors, we established a real-time polymerase chain reaction (PCR) method to examine the number of T-cell receptor-rearrangement excision circles (TRECs) in peripheral blood CD4 and CD8 T-cell populations. The real-time PCR quantitatively detected TREC sequences with a good reproducibility in human laboratory controls. In the 445 survivors so far been examined, multiple regression analysis indicated that the number of TRECs in the CD4 T-cell fraction was significantly higher in females than in males and decreased significantly with age in both males and females. This analysis also suggested a possible dose-dependent decrease in the number of TRECs in the CD4 T-cell fraction of the survivors who were less than 20 years of age at the time of bombing (p=0.09). A similar statistically significant trend for gender difference or age was observed in the CD8 T-cell fraction of the survivors. However, there was no effect of radiation exposure on the number of TRECs in the CD8-T cell fraction. The results indicate the possibility that A-bomb radiation exposure may have induced a long-term impairment in thymic CD4 T-cell production. Further investigations in a larger study population are necessary to test this hypothesis. (author)

The Population of Supernova Remnants in M51

Science.gov (United States)

Long, Knox S.; Blair, William P.; Kuntz, K. D.; Winkler, P. Frank

2017-08-01

The nearby, actively star-forming, nearly face-on spiral galaxy, M51 (NGC 5194/5), has been the site of four supernovae since 1941. As a result it should have a rich population of young supernova remnants (SNRs). Here we describe a search for optical SNRs in M51 among the 298 X-ray sources discovered inside the D25 contour in deep Chandra observations. The search uses interference filter images obtained with the WFC3 on Hubble Space Telescope and more recent images from GMOS on Gemini North. Of 80 emission nebulae identified in the HST images as SNR candidates based on elevated [SII]: Ha ratios compared to HII regions, 40 have X-ray counterparts. The diameters of the SNRs and SNR candidates detected with HST are systematically smaller than seen in SNR populations of other galaxies at comparable distances. However, this is most likely an instrumental effect, which our ongoing analysis of the new GMOS images will correct. At that point, we will be able to make of fair multi-wavelength comparison of the SNR population in M51 with other nearby, actively star-forming spiral galaxies, such as M83 and NGC6946.

Estofiili mõtteid / Edward Lucas ; tõlk. Erik Aru

Index Scriptorium Estoniae

Lucas, Edward

2008-01-01

Ajakirja The Economist ajakirjanik on seisukohal, et pronkssõdurit kasutati parteipoliitilistel eesmärkidel ning see oli mäng tulega. Lääs tuli appi, kuid mitte tänu Eesti välisministeeriumile, vaid Venemaa jämedate vigade tõttu

Establishment of reference CD4+ T cell values for adult Indian population

Directory of Open Access Journals (Sweden)

Ray Krishnangshu

2011-10-01

Full Text Available Abstract Background CD4+ T lymphocyte counts are the most important indicator of disease progression and success of antiretroviral treatment in HIV infection in resource limited settings. The nationwide reference range of CD4+ T lymphocytes was not available in India. This study was conducted to determine reference values of absolute CD4+ T cell counts and percentages for adult Indian population. Methods A multicentric study was conducted involving eight sites across the country. A total of 1206 (approximately 150 per/centre healthy participants were enrolled in the study. The ratio of male (N = 645 to female (N = 561 of 1.14:1. The healthy status of the participants was assessed by a pre-decided questionnaire. At all centers the CD4+ T cell count, percentages and absolute CD3+ T cell count and percentages were estimated using a single platform strategy and lyse no wash technique. The data was analyzed using the Statistical Package for the Social Scientist (SPSS, version 15 and Prism software version 5. Results The absolute CD4+ T cell counts and percentages in female participants were significantly higher than the values obtained in male participants indicating the true difference in the CD4+ T cell subsets. The reference range for absolute CD4 count for Indian male population was 381-1565 cells/μL and for female population was 447-1846 cells/μL. The reference range for CD4% was 25-49% for male and 27-54% for female population. The reference values for CD3 counts were 776-2785 cells/μL for Indian male population and 826-2997 cells/μL for female population. Conclusion The study used stringent procedures for controlling the technical variation in the CD4 counts across the sites and thus could establish the robust national reference ranges for CD4 counts and percentages. These ranges will be helpful in staging the disease progression and monitoring antiretroviral therapy in HIV infection in India.

USPIO-labeling in M1 and M2-polarized macrophages: An in vitro study using a clinical magnetic resonance scanner.

Science.gov (United States)

Zini, Chiara; Venneri, Mary A; Miglietta, Selenia; Caruso, Damiano; Porta, Natale; Isidori, Andrea M; Fiore, Daniela; Gianfrilli, Daniele; Petrozza, Vincenzo; Laghi, Andrea

2018-08-01

Aim of the study was to evaluate USPIO labeling in different macrophage populations using a clinical 3.0T MR unit with optical and electron microscopy as the gold standard. Human monocytic cell line THP-1 cells were differentiated into macrophages. Afterwards, M0 macrophages were incubated with IL-4 and IL-13 in order to obtain M2 polarized macrophages or with IFN-gamma and LPS for classical macrophage activation (M1). These groups were incubated with USPIO-MR contrast agent (P904) for 36 hr; M0, M0 + P904, M1 +  P904, and M2 + P904 were analyzed in gel phantoms with a 3.0T MR scanner. m-RNA of M1 and M2 markers confirmed the polarization of THP-1-derived macrophages. M2 + P904 showed a much higher T1 signal (p <  0.0001), a significantly lower (p < 0.0001) T2* signal, and significantly higher R* (p < 0.0001) compared to the other populations. Hystological analysis confirmed higher iron content in the M2-polarized population compared to both M1-polarized (p = 0.04) and M0-P904 (p = 0.003). Ultrastructure analysis demonstrated ubiquitous localization of P904 within the cellular compartments. Our results demonstrate that a selective USPIO-labeling of different macrophage populations can be detected in vitro using the 3.0T clinical scanner. © 2017 Wiley Periodicals, Inc.

Kunstioksjonil müüdi vaid neljandik töödest

Index Scriptorium Estoniae

1999-01-01

Rahvusraamatukogus toimunud galerii Riose kunstioksjonil müüdi 45-st enampakkumisele pandud tööst ainult 11. Kalleima hinnaga müüdi Paul Raua maal "Lühikese jala vaade" (51000 krooni). Ostmata jäänud töid

Multiple dendritic cell populations activate CD4+ T cells after viral stimulation.

Directory of Open Access Journals (Sweden)

Adele M Mount

2008-02-01

Full Text Available Dendritic cells (DC are a heterogeneous cell population that bridge the innate and adaptive immune systems. CD8alpha DC play a prominent, and sometimes exclusive, role in driving amplification of CD8(+ T cells during a viral infection. Whether this reliance on a single subset of DC also applies for CD4(+ T cell activation is unknown. We used a direct ex vivo antigen presentation assay to probe the capacity of flow cytometrically purified DC populations to drive amplification of CD4(+ and CD8(+ T cells following infection with influenza virus by different routes. This study examined the contributions of non-CD8alpha DC populations in the amplification of CD8(+ and CD4(+ T cells in cutaneous and systemic influenza viral infections. We confirmed that in vivo, effective immune responses for CD8(+ T cells are dominated by presentation of antigen by CD8alpha DC but can involve non-CD8alpha DC. In contrast, CD4(+ T cell responses relied more heavily on the contributions of dermal DC migrating from peripheral lymphoid tissues following cutaneous infection, and CD4 DC in the spleen after systemic infection. CD4(+ T cell priming by DC subsets that is dependent upon the route of administration raises the possibility that vaccination approaches could be tailored to prime helper T cell immunity.

Maqȃmȃt Tasawuf dan Terapi Anti Korupsi

Directory of Open Access Journals (Sweden)

Supian Ramli

2017-07-01

Full Text Available The goal of this paper is to find a comprehensive solution about corrupt behavior with Sufism teachings approach, especially about maqȃmȃt. In this context, the author uses approaching method of thinking, understanding, and practice of Sufism values ​​as an alternative to eradicating corruption, through critical analysis in looking at all the problems that occur in growing corruption behavior, by revitalizing the role and function of Sufism in this era. The conclusion of this paper shows that maqȃmȃt tasawuf in modern life can be an alternative in eradicating corruption. Therefore, every element of society and government needs to socialize the importance of the spirit of Sufism, uphold the high moral and legal awareness in managing various efforts, the government and all aspects of this life and can override the materialistic and hedonistic life which is a major obstacle factor for the absorption of values sufistik in our life.  Keywords: Sufism, Eradicating Corruption, Maqȃmȃt Abstrak Tulisan ini bertujuan untuk mencari solusi yang komprehensif terkait prilaku korupsi melalui ajaran tasawuf  khususnya terkait maqȃmȃt. Dalam konteks ini penulis menggunakan pendekatan pemikiran, pemahaman dan pengamalan nilai-nilai tasawuf sebagai alternatif pemberantasan korupsi, melalui telaah kritis dalam melihat semua persoalan yang terjadi dalam menumbuh suburkan prilaku korupsi, dengan melakukan revitalisasi peran dan fungsi pemikiran tasawuf dalam kehidupan sekarang. Kesimpulan dari  tulisan ini menunjukan bahwa maqȃmȃt tasawuf dalam kehidupan modern dapat menjadi alternatif dalam pemberantasan korupsi. Oleh karenanya, setiap elemen masyarakat dan pemerintah perlu untuk mensosialisasikan pentingnya spirit tasawuf , menjunjung moral yang tinggi dan kesadaran hukum dalam mengelola berbagai usaha, pemerintahanan dan semua aspek kehidupan ini serta dapat mengesampingkan kehidupan materialistis dan hedonistis  yang menjadi satu faktor

Kütüphan-e Türkiye Projesi: Halk Kütüphanesi Potansiyel Kullanım Araştırması

Directory of Open Access Journals (Sweden)

Umut Al

2014-12-01

Full Text Available Bu çalışmada Kütüphan-e Türkiye Projesi kapsamında yapılan Halk Kütüphanesi Potansiyel Kullanım Araştırması sonucunda elde edilen bulgular değerlendirilmektedir. Çalışma kapsamında, “İstatistiki Bölge Birimleri Sınıflandırması, Düzey 2” çerçevesinde seçilen 26 ildeki potansiyel halk kütüphanesi kullanımı çeşitli yönlerden incelenmekte, halk kütüphanesi kullanımı ve bazı değişkenler (yaş, gelir düzeyi gibi arasındaki ilişkiler analiz edilmektedir. Çalışmada halk kütüphanesi kullanım örüntülerinin coğrafik bölgeler arasında farklılık gösterip göstermediği de araştırılmaktadır. Halk Kütüphanesi Potansiyel Kullanım Araştırması Anketi 20 Şubat 2014 ile 18 Mart 2014 tarihleri arasında Kütüphan-e Türkiye Projesi kapsamında incelenen 26 ilde 2654 kişiye uygulanmıştır. Anket 24 sorudan oluşmaktadır ve halk kütüphanelerinin kullanılma düzeyi, halk kütüphanelerinin kullanılmama nedenleri, potansiyel halk kütüphanesi kullanıcılarının bilgi ve iletişim teknolojilerine yönelik becerileri gibi konularda detaylı bilgilerin elde edilmesine olanak tanımaktadır. Çalışma sonucunda kırsal alanda yaşayanların kentsel alanda yaşayanlara göre halk kütüphanelerinden daha az yararlanma eğiliminde oldukları, halk kütüphanelerini kullanmayan kesimin yoğun olarak böyle bir gereksinime sahip olmadığı için kullanmadıkları, kütüphane kullanımının eğitim, iş durumu, yaş, gelir düzeyi, yaşanılan coğrafik bölge gibi değişkenlere bağlı olarak farklılık gösterdiği belirlenmiştir. Kütüphan-e Türkiye Projesi’nin planlama faaliyetleri açısından oldukça önemli olan potansiyel kullanıcıların hangi ücretsiz eğitimleri talep ettiği bilgisi de Halk Kütüphanesi Potansiyel Kullanım Araştırması Anketi aracılığıyla elde edilmiştir.

The Clinical usefulness of {sup 99mT}c HMPAO Leukocyte/{sup 99mT}c phytate bone marrow scintigraphy for diagnosis of prosthetic knee infection: A preliminary study

Energy Technology Data Exchange (ETDEWEB)

Jung, Kyung Pyo; Park, Ji Sun; Lee, Ah Young; Choi, Su Jung; Lee, Seok Mo; Bae, Sang Kyun [Inje Univ., Pusan Paik Hospital, Pusan (Korea, Republic of)

2012-12-15

The preferred radionuclide imaging procedure for diagnosing prosthetic joint infection is combined radiolabeled leukocyte/{sup 99mT}c sulfur colloid bone marrow scintigraphy, which has an accuracy of over 90%. Unfortunately, sulfur colloid is no longer available in South Korea. in this study, we evaluated the usefulness of {sup 99mT}c phytate, a substitute for {sup 99mT}c sulfur colloid, when combined with radiolabeled leukocyte scintigraphy in suspected prosthetic knee infections. Eleven patients (nine women, two men; mean age 72{+-}6 years) with painful knee prostheses and a suspicion of infection underwent both {sup 99mT}c phytate bone marrow scintigraphy (BMS). The combined images were interpreted as positive for infection when radioactivity in the LS at the sits of clinical interest clearly exceeded that of the BMS (discordant); they were interpreted as negative when the increased activity in the LS was consistent with an increased activity in the BMS(concordant). The final diagnosis was made with microbiological or intraoperative findings and a clinical follow up of at least 12 months. Five of eleven patients were diagnosed as having an infected prosthesis. The overall sensitivity, specificity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy of the combined LS/BMS were 100%, 83%, 83%, 100% and 91%, respectively. We find that combined {sup 99mT}c HMPAO LS/{sup 99mT}c phytate BMS shows comparable diagnostic performance to other studies utilizing sulfur colloid. Combined {sup 99mT}c HMPAO LS/{sup 99mT}c phytate BMS is therefore expected to be an acceptable alternative to combined radiolabeled LS/{sup 99ms}ulfur colloid BMS for diagnosing prosthetic knee infections.

Spontaneous reverse movement of mRNA-bound tRNA through the ribosome.

Science.gov (United States)

Konevega, Andrey L; Fischer, Niels; Semenkov, Yuri P; Stark, Holger; Wintermeyer, Wolfgang; Rodnina, Marina V

2007-04-01

During the translocation step of protein synthesis, a complex of two transfer RNAs bound to messenger RNA (tRNA-mRNA) moves through the ribosome. The reaction is promoted by an elongation factor, called EF-G in bacteria, which, powered by GTP hydrolysis, induces an open, unlocked conformation of the ribosome that allows for spontaneous tRNA-mRNA movement. Here we show that, in the absence of EF-G, there is spontaneous backward movement, or retrotranslocation, of two tRNAs bound to mRNA. Retrotranslocation is driven by the gain in affinity when a cognate E-site tRNA moves into the P site, which compensates the affinity loss accompanying the movement of peptidyl-tRNA from the P to the A site. These results lend support to the diffusion model of tRNA movement during translocation. In the cell, tRNA movement is biased in the forward direction by EF-G, which acts as a Brownian ratchet and prevents backward movement.

Prantsusmaa mälupoliitikad / Patrick Garcia ; tõlk. Triinu Tamm

Index Scriptorium Estoniae

Garcia, Patrick

2006-01-01

Ajalookirjutusest Prantsusmaal pärast revolutsiooni, elukutselise ajaloolaskonna sünnist Prantsusmaal, 11. novebmri ja 8. mai tähistamisest, okupatsiooniperioodi kriitilisest läbivaatamisest, mälukonfliktidest tänapäeva demokraatlikus ühiskonnas

CCR6 is expressed on an IL-10-producing, autoreactive memory T cell population with context-dependent regulatory function.

Science.gov (United States)

Rivino, Laura; Gruarin, Paola; Häringer, Barbara; Steinfelder, Svenja; Lozza, Laura; Steckel, Bodo; Weick, Anja; Sugliano, Elisa; Jarrossay, David; Kühl, Anja A; Loddenkemper, Christoph; Abrignani, Sergio; Sallusto, Federica; Lanzavecchia, Antonio; Geginat, Jens

2010-03-15

Interleukin (IL)-10 produced by regulatory T cell subsets is important for the prevention of autoimmunity and immunopathology, but little is known about the phenotype and function of IL-10-producing memory T cells. Human CD4(+)CCR6(+) memory T cells contained comparable numbers of IL-17- and IL-10-producing cells, and CCR6 was induced under both Th17-promoting conditions and upon tolerogenic T cell priming with transforming growth factor (TGF)-beta. In normal human spleens, the majority of CCR6(+) memory T cells were in the close vicinity of CCR6(+) myeloid dendritic cells (mDCs), and strikingly, some of them were secreting IL-10 in situ. Furthermore, CCR6(+) memory T cells produced suppressive IL-10 but not IL-2 upon stimulation with autologous immature mDCs ex vivo, and secreted IL-10 efficiently in response to suboptimal T cell receptor (TCR) stimulation with anti-CD3 antibodies. However, optimal TCR stimulation of CCR6(+) T cells induced expression of IL-2, interferon-gamma, CCL20, and CD40L, and autoreactive CCR6(+) T cell lines responded to various recall antigens. Notably, we isolated autoreactive CCR6(+) T cell clones with context-dependent behavior that produced IL-10 with autologous mDCs alone, but that secreted IL-2 and proliferated upon stimulation with tetanus toxoid. We propose the novel concept that a population of memory T cells, which is fully equipped to participate in secondary immune responses upon recognition of a relevant recall antigen, contributes to the maintenance of tolerance under steady-state conditions.

RETRACTED: Relationship between the ACE I/D gene polymorphism and T1DN susceptibility/risk of T1DM developing into T1DN in the Caucasian population.

Science.gov (United States)

Zhou, Tian-Biao; Guo, Xue-Feng; Jiang, Zongpei; Li, Hong-Yan

2015-12-01

The following article has been included in a multiple retraction: Tian-Biao Zhou, Xue-Feng Guo, Zongpei Jiang, and Hong-Yan Li Relationship between the ACE I/D gene polymorphism and T1DN susceptibility/risk of T1DM developing into T1DN in the Caucasian population Journal of Renin-Angiotensin-Aldosterone System 1470320314563425, first published on February 1, 2015 doi: 10.1177/1470320314563425 This article has been retracted at the request of the Editors and the Publisher. After conducting a thorough investigation, SAGE found that the submitting authors of a number of papers published in the Journal of the Renin-Angiotensin Aldosterone System ( JRAAS) (listed below) had supplied fabricated contact details for their nominated reviewers. The Editors accepted these papers based on the reports supplied by the individuals using these fake reviewer email accounts. After concluding that the peer review process was therefore seriously compromised, SAGE and the journal Editors have decided to retract all affected articles. Online First articles (these articles will not be published in an issue) Wenzhuang Tang, Tian-Biao Zhou, and Zongpei Jiang Association of the angiotensinogen M235T gene polymorphism with risk of diabetes mellitus developing into diabetic nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563426, first published on December 18, 2014 doi: 10.1177/1470320314563426 Tian-Biao Zhou, Hong-Yan Li, Zong-Pei Jiang, Jia-Fan Zhou, Miao-Fang Huang, and Zhi-Yang Zhou Role of renin-angiotensin-aldosterone system inhibitors in radiation nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563424, first published on December 18, 2014 doi: 10.1177/1470320314563424 Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January

Investigating seagrass in Toxoplasma gondii transmission in Florida (Trichechus manatus latirostris) and Antillean (T. m. manatus) manatees

Science.gov (United States)

Wyrosdick, Heidi M; Gerhold, Richard; Su, Chunlei; Mignucci-Giannoni, Antonio A.; Bonde, Robert K.; Chapman, Alycia; Riviera-Perez, Carla; Martinez, Jessica; Miller, Debra L.

2017-01-01

Toxoplasma gondii is a feline protozoan reported to cause morbidity and mortality in manatees and other marine mammals. Given the herbivorous nature of manatees, ingestion of oocysts from contaminated water or seagrass is presumed to be their primary mode of infection. The objectives of this study were to investigate oocyst contamination of seagrass beds in Puerto Rico and determine the seroprevalence of T. gondii in Antillean (Trichechus manatus manatus) and Florida (T. m. latirostris) manatees. Sera or plasma from Antillean (n = 5) and Florida (n = 351) manatees were tested for T. gondii antibodies using the modified agglutination test. No T. gondii DNA was detected via PCR in seagrass samples (n = 33) collected from Puerto Rico. Seroprevalence was 0%, suggesting a lower prevalence of T. gondii in these manatee populations than previously reported. This was the first study to investigate the potential oocyst contamination of the manatee diet, and similar studies are important for understanding the epidemiology of T. gondii in herbivorous marine mammals.

elevation acute myoc

African Journals Online (AJOL)

USER

2010-06-21

Jun 21, 2010 ... is up-regulated in fresh thrombi, thereby contributing to thrombus .... Few studies have evaluated the role of leukocyte subtypes in ... Weibel–Palade bodies of endothelial cells to the cell .... GMP-140, a platelet - granule ...

m/sup 7/Guanosine in tRNA of Escherichia coli

Energy Technology Data Exchange (ETDEWEB)

Shugart, L; Chastain, B

1979-01-01

The nucleoside content of six different individual tRNAs was re-investigated. Particular emphasis was placed on obtaining an accurate, quantitative estimation of the amount of the minor nucleoside m/sup 7/guanosine which was found to occur at less than one full residue in tRNA/sup Phe/(E.coli) and tRNA/sup Arg/(E. coli) but not in the other species of tRNAs examined. Several hypotheses to explain this observation are discussed.

Identification of Aquifex aeolicus tRNA (m2(2G26) methyltransferase gene.

Science.gov (United States)

Takeda, Hiroshi; Hori, Hiroyuki; Endo, Yaeta

2002-01-01

The modifications of N2,N2-dimethylguanine (m2(2)G) are found in tRNAs and rRNAs from eukarya and archaea. In tRNAs, modification at position G26 is generated by tRNA (m2(2)G26) methyltransferase, which is encoded by the corresponding gene, trm1. This enzyme catalyzes the methyl-transfer from S-adenosyl-L-methionine to the semi-conserved residue, G26, via the intermediate modified base, m2G26. Recent genome sequencing project has been reported that the putative trm1 is encoded in the genome of Aquifex aeolicus, a hyper-thermophilic eubacterium as only one exception among eubacteria. In order to confirm whether this bacterial trm1 gene product is a real tRNA (m2(2)G26) methyltransferase or not, we expressed this protein by wheat germ in vitro cell-free translation system. Our biochemical analysis clearly showed that this gene product possessed tRNA (m2(2)G26) methyltransferase activity.

Photometry and Proper Motions of M, L, and T Dwarfs from the Pan-STARRS1 3π Survey

Science.gov (United States)

Best, William M. J.; Magnier, Eugene A.; Liu, Michael C.; Aller, Kimberly M.; Zhang, Zhoujian; Burgett, W. S.; Chambers, K. C.; Draper, P.; Flewelling, H.; Kaiser, N.; Kudritzki, R.-P.; Metcalfe, N.; Tonry, J. L.; Wainscoat, R. J.; Waters, C.

2018-01-01

We present a catalog of 9888 M, L and T dwarfs detected in the Pan-STARRS1 3π Survey (PS1), covering three-quarters of the sky. Our catalog contains nearly all known objects of spectral types L0–T2 in the PS1 field, with objects as early as M0 and as late as T9, and includes PS1, 2MASS, AllWISE, and Gaia DR1 photometry. We analyze the different types of photometry reported by PS1 and use two types in our catalog in order to maximize both depth and accuracy. Using parallaxes from the literature, we construct empirical SEDs for field ultracool dwarfs spanning 0.5–12 μm. We determine typical colors of M0–T9 dwarfs and highlight the distinctive colors of subdwarfs and young objects. We combine astrometry from PS1, 2MASS, and Gaia DR1 to calculate new proper motions for our catalog. We achieve a median precision of 2.9 mas yr‑1, a factor of ≈3‑10 improvement over previous large catalogs. Our catalog contains proper motions for 2405 M6–T9 dwarfs and includes the largest set of homogeneous proper motions for L and T dwarfs published to date, 406 objects for which there were no previous measurements, and 1176 objects for which we improve upon previous literature values. We analyze the kinematics of ultracool dwarfs in our catalog and find evidence that bluer but otherwise generic late-M and L field dwarfs (i.e., not subdwarfs) tend to have tangential velocities higher than those of typical field objects. With the public release of the PS1 data, this survey will continue to be an essential tool for characterizing the ultracool dwarf population.

Response of TLD-100"T"M microtubes to two RQR3 quality radiation beams

International Nuclear Information System (INIS)

Nunes, M.G.; Villani, D.; Almeida, S.B.; Vivolo, V.; Yoriyaz, H.; Louis, G.M.J.

2016-01-01

The present work compares the response of TLD-100"T"M microcubes to two RQR 3 diagnostic radiology reference quality radiation beams, defined by IEC-61267 norm, aiming to evaluate the detectability of TLD-100"T"M energy dependence reported in literature within the same reference quality radiation range. TLD-100"T"M microcubes reproducibility is assessed through the response of a second set of TLD-100"T"M microcubes, evaluated in a second thermoluminescence reader, to the RQR 3 diagnostic radiology reference quality radiation beam implemented at the Laboratorio de Calibracao de Instrumentos of IPEN, Sao Paulo, SP, Brazil. The dependence of TLD-100"T"M microcubes TL response was not detectable in these conditions and the reproducibility of the measurements is 90,2%. (author)

The CD4+CD26-T-cell population in classical Hodgkin's lymphoma displays a distinctive regulatory T-cell profile

NARCIS (Netherlands)

Ma, Yue; Visser, Lydia; Blokzijl, Tjasso; Harms, Geert; Atayar, Cigdem; Poppema, Sibrand; van den Berg, Anke

Little is known about the gene expression profile and significance of the rosetting CD4+CD26- T cells in classical Hodgkin's lymphoma (cHL). To characterize these T cells, CD4+CD26- and CD4+CD26+ T-cell populations were sorted from lymph node (LN) cell suspensions from nodular sclerosis HL (NSHL)

Minor Electrocardiographic ST-T Change and Risk of Stroke in the General Japanese Population.

Science.gov (United States)

Ishikawa, Joji; Hirose, Hideo; Schwartz, Joseph E; Ishikawa, Shizukiyo

2018-06-25

Minor ST-T changes are frequently observed on the electrocardiogram (ECG), but the risk of stroke associated with such changes is unclear.Methods and Results:In 10,642 subjects from the Japanese general population, we evaluated minor and major ST-T changes (major ST depression ≥0.1 mV) on ECGs obtained at annual health examinations. At baseline, minor ST-T changes were found in 10.7% of the subjects and 0.5% had major ST-T changes. Minor ST-T changes were associated with older age, female gender, higher systolic blood pressure, presence of hyperlipidemia, and use of antihypertensive medication. There were 375 stroke events during the follow-up period (128.7±28.1 months). In all subjects, minor ST-T changes (HR, 2.10; 95% CI: 1.57-2.81) and major ST-T changes (HR, 8.64; 95% CI: 4.44-16.82) were associated with an increased risk of stroke, but the stroke risk associated with minor ST-T changes had borderline significance after adjustment for conventional risk factors (P=0.055). In subgroup analysis, the risk of stroke was significantly associated with minor ST-T changes in subjects who had hyperlipidemia (HR, 1.75; 95% CI: 1.15-2.67) compared to those without hyperlipidemia (HR, 1.01; 95% CI: 0.64-1.59; P for interaction=0.016), even after adjustment for ECG-diagnosed left ventricular hypertrophy. Minor ST-T changes were particularly associated with a higher risk of stroke in subjects with hyperlipidemia and this association was independent of electrocardiographic left ventricular hypertrophy.

The epidemiologic study: the serum levels of TSH, T4, T3 and autoantibodies in the Polish population

International Nuclear Information System (INIS)

Gardas, A.

1991-01-01

In 1986-1990 epidemiologic studies on the thyroid diseased frequency in the Polish population were undertaken. During this studies the serum levels of TSH, T 4 , T 3 and autoantibodies were estimated in more then 30 thousand people. TSH was estimated in 30749, T 4 in 30928, T 3 in 30621 and autoantibodies in 31265 randomly chosen people in 5 districts. We have arbitrarily established the normal range for TSH to be between 0.4-3.8 μIU/ml, for T 4 4.4-12.5 μg and for T 3 0.9-1.95 ng/ml. The tested group has been divide in 4 subgroup: girls and boys from 1 to 15 years of age of the Chernobyl accident, men and women from 15 to 50 years of age at the date of the accident. TSH values in the normal range were found in 85 to 92% of the tested population, depending on the subgroup. T 4 vales in the normal range were found in 85 to 92% of the tested groups. T 3 vales in the normal range were found in 86 to 93% of the tested groups. The absence on any kind of autoantibodies was established in 89.7% of the tested population. TSH values was above the normal range (above 3.8 μIU/ml) in 3.4% of boys, 4.3% girls, 3.2% men and 3.8% women. TSH vales below the normal range (less the 0.4 μIU/ml) were found in 4.3% boys, 5% girls, 10% men and 11.2% women. T 4 values above the normal range (higher then 12.5 μg%) were found in 12.9% boys, 12.6% girls, 4.6% men and 5.9% women. T 4 vales below the normal range (less then 4.4 μg%) were detected in 0.9% boys, 1.4% girls, 2.4% men and 2.0% women. T 3 values higher then 1.95 ng/ml were found in 10.8% of boys, 11.5% girls, 2.6% men and 3.5% women. The percentage of values above of below the arbitrarily chosen normal range depends on the age and sex group. (author). 5 refs, 4 tabs

Effect of PEG and mPEG-anthracene on tRNA aggregation and particle formation.

Science.gov (United States)

Froehlich, E; Mandeville, J S; Arnold, D; Kreplak, L; Tajmir-Riahi, H A

2012-01-09

Poly(ethylene glycol) (PEG) and its derivatives are synthetic polymers with major applications in gene and drug delivery systems. Synthetic polymers are also used to transport miRNA and siRNA in vitro. We studied the interaction of tRNA with several PEGs of different compositions, such as PEG 3350, PEG 6000, and mPEG-anthracene under physiological conditions. FTIR, UV-visible, CD, and fluorescence spectroscopic methods as well as atomic force microscopy (AFM) were used to analyze the PEG binding mode, the binding constant, and the effects of polymer complexation on tRNA stability, aggregation, and particle formation. Structural analysis showed that PEG-tRNA interaction occurs via RNA bases and the backbone phosphate group with both hydrophilic and hydrophobic contacts. The overall binding constants of K(PEG 3350-tRNA)= 1.9 (±0.5) × 10(4) M(-1), K(PEG 6000-tRNA) = 8.9 (±1) × 10(4) M(-1), and K(mPEG-anthracene)= 1.2 (±0.40) × 10(3) M(-1) show stronger polymer-RNA complexation by PEG 6000 and by PEG 3350 than the mPEG-anthracene. AFM imaging showed that PEG complexes contain on average one tRNA with PEG 3350, five tRNA with PEG 6000, and ten tRNA molecules with mPEG-anthracene. tRNA aggregation and particle formation occurred at high polymer concentrations, whereas it remains in A-family structure.

RETRACTED: Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population.

Science.gov (United States)

Zhong, Weiqiang; Jiang, Zongpei; Zhou, Tian-Biao

2015-12-01

This article has been included in a multiple retraction: Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January 26, 2015 doi: 10.1177/1470320314566019 This article has been retracted at the request of the Editors and the Publisher. After conducting a thorough investigation, SAGE found that the submitting authors of a number of papers published in the Journal of the Renin-Angiotensin Aldosterone System ( JRAAS) (listed below) had supplied fabricated contact details for their nominated reviewers. The Editors accepted these papers based on the reports supplied by the individuals using these fake reviewer email accounts. After concluding that the peer review process was therefore seriously compromised, SAGE and the journal Editors have decided to retract all affected articles. Online First articles (these articles will not be published in an issue) Wenzhuang Tang, Tian-Biao Zhou, and Zongpei Jiang Association of the angiotensinogen M235T gene polymorphism with risk of diabetes mellitus developing into diabetic nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563426, first published on December 18, 2014 doi: 10.1177/1470320314563426 Tian-Biao Zhou, Hong-Yan Li, Zong-Pei Jiang, Jia-Fan Zhou, Miao-Fang Huang, and Zhi-Yang Zhou Role of renin-angiotensin-aldosterone system inhibitors in radiation nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563424, first published on December 18, 2014 doi: 10.1177/1470320314563424 Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January 26, 2015 doi: 10

Märkmeid surnud majast : tänase Euroopa mälu / Tony Judt ; tõlk. Märt Väljataga

Index Scriptorium Estoniae

Judt, Tony, 1948-2010

2006-01-01

Erinevustest holokausti mäletamises Lääne- ja Ida-Euroopas. Miks erinevad venelaste ja Venemaa satelliitriikide mälestused Teisest maailmasõjast. Venelaste lõhenenud mälust - kodanikuühendustest "Memoriaal" ja "Pamjat". Kiusatusest ületada kommunismimälestus selle peapeale pööramisega. Ebamugavast tõest, et enamiku inimeste jaoks ei olnud 1939. ja 1945. a. vahel juutidega toimunu tegelikult tähtis.

Distribution of espM and espT among enteropathogenic and enterohaemorrhagic Escherichia coli

Science.gov (United States)

Arbeloa, Ana; Blanco, Miguel; Moreira, Fabiana C.; Bulgin, Richard; López, Cecilia; Dahbi, Ghizlane; Blanco, Jesús E.; Mora, Azucena; Alonso, María Pilar; Mamani, Rosalia Ceferina; Gomes, Tânia A. T.; Blanco, Jorge; Frankel, Gad

2009-01-01

Enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) translocate dozens of type III secretion system effectors, including the WxxxE effectors Map, EspM and EspT that activate Rho GTPases. While map, which is carried on the LEE pathogenicity island, is absolutely conserved among EPEC and EHEC strains, the prevalence of espM and espT is not known. Here we report the results of a large screen aimed at determining the prevalence of espM and espT among clinical EPEC and EHEC isolates. The results suggest that espM, detected in 51 % of the tested strains, is more commonly found in EPEC and EHEC serogroups that are linked to severe human infections. In contrast, espT was absent from all the EHEC isolates and was found in only 1.8 % of the tested EPEC strains. Further characterization of the virulence gene repertoire of the espT-positive strains led to the identification of a new ζ2 intimin variant. All the espT-positive strains but two contained the tccP gene. espT was first found in Citrobacter rodentium and later in silico in EPEC E110019, which is of particular interest as this strain was responsible for a particularly severe diarrhoeal outbreak in Finland in 1987 that affected 650 individuals in a school complex and an additional 137 associated household members. Comparing the protein sequences of EspT to that of E110019 showed a high level of conservation, with only three strains encoding EspT that differed in 6 amino acids. At present, it is not clear why espT is so rare, and what impact EspM and EspT have on EPEC and EHEC infection. PMID:19528152

Estudo do comportamento térmico de laminados carbono/epóxi submetidos a múltiplos ciclos térmicos

Directory of Open Access Journals (Sweden)

Christiane Sales Reis de Souza

2016-01-01

Full Text Available Resumo Este trabalho apresenta um estudo comparativo dos comportamentos térmicos de três famílias de laminados de fibra de carbono/resina epóxi, submetidas a múltiplos ciclos térmicos, via análises termogravimétricas e dinâmico-mecânicas. Este estudo procura aliar a possibilidade de utilização de retalhos de pré-impregnados de fibra de carbono com resina epóxi - um material nobre na indústria aeronáutica, por meio da comparação do comportamento térmico de um laminado preparado com retalhos de pré-impregnados (laminado R, com outros dois laminados, sendo um manufaturado com prepreg comercial (laminado A e um terceiro obtido por impregnação manual de reforço seco de fibra de carbono com resina epóxi (laminado B. Os laminados curados a 180 °C foram submetidos a múltiplos ciclos térmicos, totalizando 40, 100 e 300 h de exposição na temperatura máxima de 180 °C. As análises termogravimétricas mostram que os laminados submetidos às múltiplas ciclagens apresentam estabilidade térmica na temperatura de 180 °C, com perda de massa relativa à umidade absorvida pelos laminados. As análises dinâmico-mecânicas evidenciam que as múltiplas ciclagens térmicas afetam pouco ou até aumentam as temperaturas de transição vítrea dos sistemas de resinas epóxis estudados. A partir dos resultados obtidos pode-se afirmar que os laminados submetidos às múltiplas ciclagens térmicas apresentam estabilidade térmica suficiente para serem utilizados, por exemplo, em ferramentais de cura de componentes aeronáuticos.

mIoT Slice for 5G Systems: Design and Performance Evaluation.

Science.gov (United States)

Trivisonno, Riccardo; Condoluci, Massimo; An, Xueli; Mahmoodi, Toktam

2018-02-21

Network slicing is a key feature of the upcoming 5G networks allowing the design and deployment of customized communication systems to integrate services provided by vertical industries. In this context, massive Internet of Things (mIoT) is regarded as a compelling use case, both for its relevance from business perspective, and for the technical challenges it poses to network design. With their envisaged massive deployment of devices requiring sporadic connectivity and small data transmission, yet Quality of Service (QoS) constrained, mIoT services will need an ad-hoc end-to-end (E2E) slice, i.e., both access and core network with enhanced Control and User planes (CP/UP). After revising the key requirements of mIoT and identifying major shortcomings of previous generation networks, this paper presents and evaluates an E2E mIoT network slicing solution, featuring a new connectivity model overcoming the load limitations of legacy systems. Unique in its kind, this paper addresses mIoT requirements from an end-to-end perspective highlighting and solving, unlike most prior related work, the connectivity challenges posed to the core network. Results demonstrate that the proposed solution, reducing CP signaling and optimizing UP resource utilization, is a suitable candidate for next generation network standards to efficiently handle massive device deployment.

Otsustusõigus töötajatele!? / Lawrence M. Fisher ; tõlk. Kadi Tarand

Index Scriptorium Estoniae

Fisher, Lawrence M.

2007-01-01

Semco Grupi peadirektor Ricardo Semler on ettevõtte juhtimises praktiseerinud uut meetodit, mille puhul töölised on kaasatud oluliste otsuste tegemisse. Osalusjuhtimine ja korporatiivdemokraatia. Kommenteerivad: Kaspars Kaulinsh. Semleri meetodi rakendamiseks tuleb pidada kaks olulist võitlust; Alo Lillepea. Kuus inimest kümnest ei mõista Semlerit

Idaho National Laboratory Technology to Market (T2M) Final Report

Energy Technology Data Exchange (ETDEWEB)

Wright, Christopher Todd [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bush, Jason William [Idaho National Lab. (INL), Idaho Falls, ID (United States); Gentle, Jake Paul [Idaho National Lab. (INL), Idaho Falls, ID (United States); Hill, Porter Jack [Idaho National Lab. (INL), Idaho Falls, ID (United States); Myers, Kurt Steven [Idaho National Lab. (INL), Idaho Falls, ID (United States); Williams, Christopher Luke [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-01-01

The objective of this project is to establish a tiered Technology to Market (T2M) curriculum for basic researchers to project leads to measure the effect of technology transfer skills on project success and impact. The plan will train five researchers in basic technology transfer principles where success will be measured by assessing improvements in T2M skills and knowledge after the training is complete, likely using before and after surveys.

Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups.

Science.gov (United States)

de Oliveira Hiragi, Cássia; Miranda-Vilela, Ana Luisa; Rocha, Dulce Maria Sucena; de Oliveira, Silviene Fabiana; Hatagima, Ana; de Nazaré Klautau-Guimarães, Maria

2011-01-01

Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution of these variants. We described the distribution frequency of CAT (21A/T), SOD2 (Ala9Val), GPX1 (Pro198Leu), GSTM1 and GSTT1 polymorphisms in three Brazilian population groups: Kayabi Amerindians (n = 60), Kalunga Afro-descendants (n = 72), and an urban mixed population from Federal District (n = 162). Frequencies of the variants observed in Kalunga (18% to 58%) and Federal District (33% to 63%) were similar to those observed in Euro and Afro-descendants, while in Kayabi (3% to 68%), depending on the marker, frequencies were similar to the ones found in different ethnic groups. Except for SOD2 in all population groups studied here, and for GPX1 in Kalunga, the genotypic distributions were in accordance with Hardy-Weinberg Equilibrium. These data can clarify the contribution of different ethnicities in the formation of mixed populations, such as that of Brazil. Moreover, outcomes will be valuable resources for future functional studies and for genetic studies in specific populations. If these studies are designed to comprehensively explore the role of these genetic polymorphisms in the etiology of human diseases they may help to prevent inconsistent genotype-phenotype associations in pharmacogenetic studies.

High frequency of mutation G377S in Brazilian type 3 Gaucher disease patients

Directory of Open Access Journals (Sweden)

R. Rozenberg

2006-09-01

Full Text Available Gaucher disease (GD, the most prevalent lysosome storage disorder, presents an autosomal recessive mode of inheritance. It is a paradigm for therapeutic intervention in medical genetics due to the existence of effective enzyme replacement therapy. We report here the analysis of GD in 262 unrelated Brazilian patients, carried out in order to establish the frequency of the most common mutations and to provide prognostic information based on genotype-phenotype correlations. Among 247 type 1 GD patients, mutation N370S was detected in 47% of all the alleles, but N370S/N370S homozygosity was found in only 10% of the patients, a much lower frequency than expected, suggesting that most individuals presenting this genotype may not receive medical attention. Recombinant alleles were detected at a high frequency: 44% of the chromosomes bearing mutation L444P had other mutations derived from the pseudogene sequence, present in 25% of patients. Three neuronopathic type 2 patients were homozygous for L444P, all presenting additional mutations (E326K or recombinant alleles that probably lead to the more severe phenotypes. Six children, classified as type 1 GD patients, had a L444P/L444P genotype, showing that neuronopathic symptoms may only manifest later in life. This would indicate the need for a higher treatment dose during enzyme replacement therapy. Finally, mutation G377S was present in 4 homozygous type 1 patients and also in compound heterozygosity in 5 (42% type 3 patients. These findings indicate that G377S cannot be unambiguously classified as mild and suggest an allele-dose effect for this mutation.

Development of a spirometry T-score in the general population.

Science.gov (United States)

Lee, Sei Won; Kim, Hyun Kuk; Baek, Seunghee; Jung, Ji-Ye; Kim, Young Sam; Lee, Jae Seung; Lee, Sang-Do; Mannino, David M; Oh, Yeon-Mok

2016-01-01

Spirometry values may be expressed as T-scores in standard deviation units relative to a reference in a young, normal population as an analogy to the T-score for bone mineral density. This study was performed to develop the spirometry T-score. T-scores were calculated from lambda-mu-sigma-derived Z-scores using a young, normal age reference. Three outcomes of all-cause death, respiratory death, and COPD death were evaluated in 9,101 US subjects followed for 10 years; an outcome of COPD-related health care utilization (COPD utilization) was evaluated in 1,894 Korean subjects followed for 4 years. The probability of all-cause death appeared to remain nearly zero until -1 of forced expiratory volume in 1 second (FEV1) T-score but increased steeply where FEV1 T-score reached below -2.5. Survival curves for all-cause death, respiratory death, COPD death, and COPD utilization differed significantly among the groups when stratified by FEV1 T-score (Pspirometry T-score could predict all-cause death, respiratory death, COPD death, and COPD utilization.

The dual specificity PI3K/mTOR inhibitor PKI-587 displays efficacy against T-cell acute lymphoblastic leukemia (T-ALL).

Science.gov (United States)

Gazi, Mohiuddin; Moharram, Sausan A; Marhäll, Alissa; Kazi, Julhash U

2017-04-28

Although significant improvements have been made in the treatment of acute lymphoblastic leukemia (ALL), there is a substantial subset of high-risk T-cell ALL (T-ALL) patients with relatively poor prognosis. Like in other leukemia types, alterations of the PI3K/mTOR pathway are predominant in ALL which is also responsible for treatment failure and relapse. In this study, we show that relapsed T-ALL patients display an enrichment of the PI3K/mTOR pathway. Using a panel of inhibitors targeting multiple components of the PI3K/mTOR pathway, we observed that the dual-specific PI3K/mTOR inhibitor PKI-587 was the most selective inhibitor for T-ALL cells dependent on the PI3K/mTOR pathway. Furthermore, we observed that PKI-587 blocked proliferation and colony formation of T-ALL cell lines. Additionally, PKI-587 selectively abrogated PI3K/mTOR signaling without affecting MAPK signaling both in in vitro and in vivo. Inhibition of the PI3K/mTOR pathway using PKI-587 delayed tumor progression, reduced tumor load and enhanced the survival rate in immune-deficient mouse xenograft models without inducing weight loss in the inhibitor treated mice. This preclinical study shows beneficial effects of PKI-587 on T-ALL that warrants further investigation in the clinical setting. Copyright © 2017 Elsevier B.V. All rights reserved.

A Role for M-Matrices in Modelling Population Growth

Science.gov (United States)

James, Glyn; Rumchev, Ventsi

2006-01-01

Adopting a discrete-time cohort-type model to represent the dynamics of a population, the problem of achieving a desired total size of the population under a balanced growth (contraction) and the problem of maintaining the desired size, once achieved, are studied. Properties of positive-time systems and M-matrices are used to develop the results,…

~ i t i d e r m a t o ~ h ~ t i c Activities of Nine (9) Essential Oils J.R. ...

African Journals Online (AJOL)

i t i d e r m a t o ~ h ~ t i c Activities of Nine (9) Essential Oils. J.R. KUIATE", S.P. KUATE'.~, N.E. KEMADJOU~, S. DJOKOUA~, F. ZIFACK~ AND J. ~. 0. ~. 0. ~. I Department of Biochemistry, FS, University of Dschang, P.O. Box 67 Dschang, Cameroon. 2~epartment of Biochemistry, FS, University of Yaoundt!, P.O. Box 812 ...

C677T (RS1801133 ) MTHFR gene polymorphism frequency in a colombian population.

Science.gov (United States)

Romero-Sánchez, Consuelo; Gómez-Gutierrez, Alberto; Gómez, Piedad Elena; Casas-Gomez, Maria Consuelo; Briceño, Ignacio

2015-01-01

Abnormal levels of the enzyme methylenetetrahydrofolate reductase (MTHFR) are associated with an increased risk of both cardiovascular and cerebrovascular disease and higher concentrations of homocysteine. Abnormal levels are also related to birth defects, pregnancy complications, cancer and toxicity to methotrexate (MTX). Polymorphisms of MTHFR affect the activity of the enzyme. Genetic associations have been related to treatment efficacy. To establish the frequency of the C> T polymorphism at nucleotide 677 of the MTHFR gene in a group of Colombian individuals. Data from pharmacogenetic microarrays that include MTX sensibility-associated polymorphisms were retrospectively collected (Pathway Genomics(®)). The frequency of the C> T MTHFR rs1801133 marker polymorphism was analyzed. Microarray data from 68 men and 84 women were analyzed. Comparisons of genotype C/C vs. C/T and T/T were statistically significantly different (p= 0.00, p= 0.026, respectively), as were C/T and T / T (p= 0.0001). Results for the C/C and C/T genotypes in a Colombian population are similar to other previously studied groups of healthy subjects. Subjects from our population might be at risk of developing diseases associated with MTHFR polymorphisms and might present toxicity and adverse effects if treated with MTX, which suggests the need to evaluate therapeutic alternatives based on individual pharmacogenetic studies.

Glutathione S-transferase M1 and T1 null genotype frequency ...

Indian Academy of Sciences (India)

PREM CHANDRA SUTHAR

2018-03-24

Mar 24, 2018 ... A comparative analysis with different tribal as well as world population has also been ...... (GSTM1, T1 and P1) gene polymorphisms with type 2 diabetes mellitus .... from the Spanish Bladder Cancer Study and meta-analyses.

Biophysical characterization of the olfactomedin domain of myocilin, an extracellular matrix protein implicated in inherited forms of glaucoma.

Directory of Open Access Journals (Sweden)

Susan D Orwig

Full Text Available Myocilin is an eye protein found in the trabecular extracellular matrix (TEM, within the anatomic region that controls fluid flow. Variants of myocilin, localized to its olfactomedin (OLF domain, have been linked to inherited forms of glaucoma, a disease associated with elevated intraocular pressure. OLF domains have also been implicated in psychiatric diseases and cancers by their involvement in signaling, neuronal growth, and development. However, molecular characterization of OLFs has been hampered by challenges in recombinant expression, a hurdle we have recently overcome for the myocilin OLF domain (myoc-OLF. Here, we report the first detailed solution biophysical characterization of myoc-OLF to gain insight into its structure and function. Myoc-OLF is stable in the presence of glycosaminoglycans, as well as in a wide pH range in buffers with functional groups reminiscent of such glycosaminoglycans. Circular dichroism (CD reveals significant β-sheet and β-turn secondary structure. Unexpectedly, the CD signature is reminiscent of α-chymotrypsin as well as another ocular protein family, the βγ-crystallins. At neutral pH, intrinsic tryptophan fluorescence and CD melts indicate a highly cooperative transition with a melting temperature of ∼55 °C. Limited proteolysis combined with mass spectrometry reveals that the compact core structural domain of OLF consists of approximately residues 238-461, which retains the single disulfide bond and is as stable as the full myoc-OLF construct. The data presented here inform new testable hypotheses for interactions with specific TEM components, and will assist in design of therapeutic agents for myocilin glaucoma.

Resolving combinatorial ambiguities in dilepton t t¯ event topologies with constrained M2 variables

Science.gov (United States)

Debnath, Dipsikha; Kim, Doojin; Kim, Jeong Han; Kong, Kyoungchul; Matchev, Konstantin T.

2017-10-01

We advocate the use of on-shell constrained M2 variables in order to mitigate the combinatorial problem in supersymmetry-like events with two invisible particles at the LHC. We show that in comparison to other approaches in the literature, the constrained M2 variables provide superior ansätze for the unmeasured invisible momenta and therefore can be usefully applied to discriminate combinatorial ambiguities. We illustrate our procedure with the example of dilepton t t ¯ events. We critically review the existing methods based on the Cambridge MT 2 variable and MAOS reconstruction of invisible momenta, and show that their algorithm can be simplified without loss of sensitivity, due to a perfect correlation between events with complex solutions for the invisible momenta and events exhibiting a kinematic endpoint violation. Then we demonstrate that the efficiency for selecting the correct partition is further improved by utilizing the M2 variables instead. Finally, we also consider the general case when the underlying mass spectrum is unknown, and no kinematic endpoint information is available.

C677T (RS1801133 ) MTHFR gene polymorphism frequency in a colombian population

Science.gov (United States)

Gómez-Gutierrez, Alberto; Gómez, Piedad Elena; Casas-Gomez, Maria Consuelo; Briceño, Ignacio

2015-01-01

Introduction: Abnormal levels of the enzyme methylenetetrahydrofolate reductase (MTHFR) are associated with an increased risk of both cardiovascular and cerebrovascular disease and higher concentrations of homocysteine. Abnormal levels are also related to birth defects, pregnancy complications, cancer and toxicity to methotrexate (MTX). Polymorphisms of MTHFR affect the activity of the enzyme. Genetic associations have been related to treatment efficacy. Objective: To establish the frequency of the C> T polymorphism at nucleotide 677 of the MTHFR gene in a group of Colombian individuals. Methods: Data from pharmacogenetic microarrays that include MTX sensibility-associated polymorphisms were retrospectively collected (Pathway Genomics®). The frequency of the C> T MTHFR rs1801133 marker polymorphism was analyzed. Results: Microarray data from 68 men and 84 women were analyzed. Comparisons of genotype C/C vs. C/T and T/T were statistically significantly different (p= 0.00, p= 0.026, respectively), as were C/T and T / T (p= 0.0001). Conclusions: Results for the C/C and C/T genotypes in a Colombian population are similar to other previously studied groups of healthy subjects. Subjects from our population might be at risk of developing diseases associated with MTHFR polymorphisms and might present toxicity and adverse effects if treated with MTX, which suggests the need to evaluate therapeutic alternatives based on individual pharmacogenetic studies. PMID:26309343

2018-05-05T21:20:14Z https://www.ajol.info/index.php/all/oai oai:ojs ...

African Journals Online (AJOL)

article/37925 2018-05-05T21:20:14Z cajeb:ART Macrophyte diversity in polluted and non-polluted wetlands in Cameroon Fonkou, Théophile; BP 377 Dschang, Cameroon Nguetsop, Victor F Pinta, Jonas Y Dekoum, Vincent MA Lekeufack, Martin ...

Ultracool Subdwarfs: Metal-poor Stars and Brown Dwarfs Extending into the Late-type M, L and T Dwarf Regimes

OpenAIRE

Burgasser, Adam J.; Kirkpatrick, J. Davy; Lepine, Sebastien

2004-01-01

Recent discoveries from red optical proper motion and wide-field near-infrared surveys have uncovered a new population of ultracool subdwarfs -- metal-poor stars and brown dwarfs extending into the late-type M, L and possibly T spectral classes. These objects are among the first low-mass stars and brown dwarfs formed in the Galaxy, and are valuable tracers of metallicity effects in low-temperature atmospheres. Here we review the spectral, photometric, and kinematic properties of recent discov...

Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma

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Fernando A Monteiro

1999-09-01

Full Text Available DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes were genetically identical.

Somaatiline mõiste KÄSI tähendusmuutuste esiletoojana / Silvi Tenjes

Index Scriptorium Estoniae

Tenjes, Silvi, 1958-

2007-01-01

Vaadeldakse eesti- ja venekeelseid "käe" mõistega seotud väljendeid Mihhail Bulgakovi "Meistris ja Margaritas" ning uuritakse, kas saadud tulemused võiksid peegeldada sotsiokultuurilisi mõjusid keele tähendussfäärile juba rahvaste rändamise ajast alates

2'-O-methylation in mRNA disrupts tRNA decoding during translation elongation.

Science.gov (United States)

Choi, Junhong; Indrisiunaite, Gabriele; DeMirci, Hasan; Ieong, Ka-Weng; Wang, Jinfan; Petrov, Alexey; Prabhakar, Arjun; Rechavi, Gideon; Dominissini, Dan; He, Chuan; Ehrenberg, Måns; Puglisi, Joseph D

2018-03-01

Chemical modifications of mRNA may regulate many aspects of mRNA processing and protein synthesis. Recently, 2'-O-methylation of nucleotides was identified as a frequent modification in translated regions of human mRNA, showing enrichment in codons for certain amino acids. Here, using single-molecule, bulk kinetics and structural methods, we show that 2'-O-methylation within coding regions of mRNA disrupts key steps in codon reading during cognate tRNA selection. Our results suggest that 2'-O-methylation sterically perturbs interactions of ribosomal-monitoring bases (G530, A1492 and A1493) with cognate codon-anticodon helices, thereby inhibiting downstream GTP hydrolysis by elongation factor Tu (EF-Tu) and A-site tRNA accommodation, leading to excessive rejection of cognate aminoacylated tRNAs in initial selection and proofreading. Our current and prior findings highlight how chemical modifications of mRNA tune the dynamics of protein synthesis at different steps of translation elongation.

A study of the M235T variant of the angiotensinogen gene and ...

African Journals Online (AJOL)

Mary E. Kooffreh

2012-08-11

Aug 11, 2012 ... sion analysis were used to analyze the data obtained. ... interval: 0.39–1.06) there were no significant differences between the genotype ... of the M235T variant was high, it was not a significant risk factor for ... Before taking the measurement, the .... Distribution of the AGT M235T allele by gender in the.

Population density approach for discrete mRNA distributions in generalized switching models for stochastic gene expression.

Science.gov (United States)

Stinchcombe, Adam R; Peskin, Charles S; Tranchina, Daniel

2012-06-01

We present a generalization of a population density approach for modeling and analysis of stochastic gene expression. In the model, the gene of interest fluctuates stochastically between an inactive state, in which transcription cannot occur, and an active state, in which discrete transcription events occur; and the individual mRNA molecules are degraded stochastically in an independent manner. This sort of model in simplest form with exponential dwell times has been used to explain experimental estimates of the discrete distribution of random mRNA copy number. In our generalization, the random dwell times in the inactive and active states, T_{0} and T_{1}, respectively, are independent random variables drawn from any specified distributions. Consequently, the probability per unit time of switching out of a state depends on the time since entering that state. Our method exploits a connection between the fully discrete random process and a related continuous process. We present numerical methods for computing steady-state mRNA distributions and an analytical derivation of the mRNA autocovariance function. We find that empirical estimates of the steady-state mRNA probability mass function from Monte Carlo simulations of laboratory data do not allow one to distinguish between underlying models with exponential and nonexponential dwell times in some relevant parameter regimes. However, in these parameter regimes and where the autocovariance function has negative lobes, the autocovariance function disambiguates the two types of models. Our results strongly suggest that temporal data beyond the autocovariance function is required in general to characterize gene switching.

PROMIS Physical Function Correlation With NDI and mJOA in the Surgical Cervical Myelopathy Patient Population.

Science.gov (United States)

Owen, Robert J; Zebala, Lukas P; Peters, Colleen; McAnany, Steven

2018-04-15

Retrospective review. To determine the correlation of Patient-Reported Outcomes Measurement Information System (PROMIS) physical function with Neck Disability Index (NDI) and Modified Japanese Orthopedic Association (mJOA) scores in the surgical cervical myelopathy patient population. Outcome measures such as NDI and mJOA are essential for analyzing treatments for cervical myelopathy. Administrative burdens impose limits on completion of these measures. The PROMIS group developed an outcome measure to improve reporting of patient symptoms and function and to reduce administrative burden. Despite early success, NDI and mJOA have not been compared with PROMIS in patients with cervical myelopathy. This study determines the correlation of NDI and mJOA with PROMIS in surgical patients with cervical myelopathy. A total of 60 patients with cervical myelopathy undergoing surgery were included. PROMIS, NDI, and mJOA were collected preoperatively, and in the first 6 months postoperatively. Correlations between NDI, mJOA, and PROMIS were quantified using Pearson correlation coefficients. Students t tests were used to test significance. All 60 (100%) of patients completed preoperative questionnaires. Fifty-five (92%) of patients completed initial follow-up questionnaires within the first 6 months. PROMIS physical function and NDI demonstrated a strong negative correlation at baseline and in initial follow-up (R = -0.69, -0.76). PROMIS and mJOA demonstrated a strong positive correlation at baseline and in initial follow-up (R = 0.61, 0.72). PROMIS physical function has a strong negative correlation with NDI and a strong positive correlation with mJOA at baseline and in the early postoperative course in patients undergoing surgery for cervical myelopathy. Surgeons may factor these outcomes into the delivery and interpretation of patient-reported outcome measures in this population. Use of PROMIS may improve completion of outcome measures in the office and reduce

Somatická analýza posluchačů tělesné výchovy ve čtyřletém sledovaném období Somatic analysis of PE students within a four-year research period

Directory of Open Access Journals (Sweden)

Vojtěch Gajda

2006-02-01

Full Text Available Moderní pojetí interpretuje sportovní výkon jako vymezený systém prvků, které jsou označovány jako komponenty, determinanty, faktory apod. Velmi důležitou skupinou faktorů jsou faktory somatické, které se týkají podpůrného systému. Za somatické faktory považujeme tělesnou výšku, hmotnost, délkové, šířkové a obvodové rozměry a z nich tedy plynoucí tělesnou stavbu (velmi často vyjádřenou somatotypem. Příspěvek se zabývá tělesnou stavbou studentů oboru TV 1. ročníku, které můžeme považovat za selektovanou skupinu populace s poměrně velkým objemem pohybové aktivity. Šetření bylo prováděno v letech 2001–2004 a bylo do něj zařazeno 265 studentů (166 mužů a 99 žen. Pro zjištění tělesné stavby byl určován somatotyp podle metodiky Heath-Certer (Riegerová & Ulbrichová, 1993. Při srovnání průměrných somatotypů studentů tělesné výchovy v jednotlivých letech jsme nezaznamenali významné rozdíly. Za výjimku můžeme považovat snad jen endomorfní komponentu u mužů, která měla výrazně klesající trend (hodnota se blížila k hranici statistické významnosti α = 0,05. Vzhledem k tomu, že považujeme studenty tělesné výchovy za selektovanou populaci s velkým objemem pohybové aktivity, předpokládali jsme u nich výrazný rozvoj habitu a typ somatotypu, kterým se většina přiblíží optimálnímu somatotypu pro studenty TV (Riegerová & Ulbrichová, 1993. Měření však ukázala, že studenti výrazně zaostávají především v oblasti mezomorfie, což ukazuje také srovnání s populačním normativem. Domníváme se, že zjištěné hodnoty mohou vypovídat o klesající úrovni fyzického a s tím souvisejícího somatického rozvoje naší populace. Modern theories and studies interpret sport performance as a defined system of elements which are characterized as components, determiners, factors, etc. Somatic factors concerning the support system

T his article is m ainly about using sim ple trigonom etric identities ...

Indian Academy of Sciences (India)

T his article is m ainly about using sim ple trigonom etric identities and basic calculus to deduce som e new and interesting series. In particular, I use basic aspects of. G am m a and B eta integrations to deduce these series. T hese lead to interesting series for ¼ or functions of ¼ . O ne form ofbeta integration w hich is often ...

MD SIMULATION STUDIES TO INVESTIGATE ISO-ENERGETIC CONFORMATIONAL BEHAVIOUR OF MODIFIED NUCLEOSIDES M2G AND M22G PRESENT IN tRNA

Directory of Open Access Journals (Sweden)

Rohit S Bavi

2013-02-01

Full Text Available Modified nucleic acid bases are most commonly found in tRNA. These may contain modifications from simple methylation to addition of bulky groups. Methylation of the four canonical nucleotide bases at a wide variety of positions is particularly prominent among the known modification. Methylation of N2 group of guanine is a relatively common modification in tRNA and rRNA. N2-methylguanosine (m2G is the second most often encountered nucleoside in E. coli tRNAs. N2, N2-dimethylguanosine (m22G is found in the majority of eukaryotic tRNAs and involved in forming base pair interactions with adjacent bases. Hence, in order to understand the structural significance of these methylated nucleic acid bases we have carried out molecular dynamics simulation to see the salvation effect. The results obtained shows iso-energetic conformational behaviors for m2G and m22G. The simulation trajectory of m2G shows regular periodical fluctuations suggesting that m2G is equally stable as either s-cis or s-trans rotamers. The two rotamers of m2G may interact canonically or non-canonically with opposite base as s-trans m2G26:C/A/U44 and s-cis m2G26:A/U44. The free rotations around the C-N bond could be the possible reason for these iso-energetic conformations. Dimethylation of G has almost no influence on base pairing with either A or U. Thus, these results reveal that modified nucleosides m2G and m22G may play an important role to prevent tRNA from adopting the unusual mitochondrial like conformation.

Microwave measurements of the time evolution of electron density in the T-11M tokamak

International Nuclear Information System (INIS)

Petrov, V.G.; Petrov, A.A.; Malyshev, A.Yu.; Markov, V.K.; Babarykin, A.V.

2004-01-01

Unambiguous diagnostics intended for measuring the time behavior of the electron density and monitoring the line-averaged plasma density in the T-11M tokamak are described. The time behavior of the plasma density in the T-11M tokamak is measured by a multichannel phase-jump-free microwave polarization interferometer based on the Cotton-Mouton effect. After increasing the number of simultaneously operating interferometer channels and enhancing the sensitivity of measurements, it became possible to measure the time evolution of the plasma density profile in the T-11M tokamak. The first results from such measurements in various operating regimes of the T-11M tokamak are presented. The measurement and data processing techniques are described, the measurement errors are analyzed, and the results obtained are discussed. We propose using a pulsed time-of-flight refractometer to monitor the average plasma density in the T-11M tokamak. The refractometer emits nanosecond microwave probing pulses with a carrier frequency that is higher than the plasma frequency and, thus, operates in the transmission mode. A version of the instrument has been developed with a carrier frequency of 140 GHz, which allows one to measure the average density in regimes with a nominal T-11M plasma density of (3-5) x 10 13 cm -3 . Results are presented from the first measurements of the average density in the T-11M tokamak with the help of a pulsed time-of-flight refractometer by probing the plasma in the equatorial plane in a regime with the reflection of the probing radiation from the inner wall of the vacuum chamber

3M"T"M neutron quench. Compounds with substantial water solubility and boron content

International Nuclear Information System (INIS)

Cook, Kevin S.; Blake, Alex B.; Neef, C. Jody

2014-01-01

Of the two naturally occurring isotopes of boron ("1"1B 80%, "1"0B 20%), "1"0B is a good neutron absorber with a thermal neutron absorption cross section of ∼3800 barns. The ability to absorb thermal neutrons while producing benign reaction products makes boron an ideal atom to aid in the control and arrest of the fission reaction in nuclear power reactors. In current practice, boric acid and sodium pentaborate are commonly used as neutron absorbers in the water regime of active and passive safety systems. 3M"T"M Neutron Quench compounds have been developed to be applied in situations where criticality control needs exceed normal control methods. In this type of situation these compounds have several advantages over commonly used neutron absorbers like boric acid: Boron Content; compounds contain up to 80 wt% boron compared to 16 wt% for boric acid and sodium pentaborate. Solubility; >16 g B/100 g solution compared to 0.6 g B/100 g solution for boric acid at 25°C. pH neutrality; compounds demonstrate pH neutrality even in concentrated solutions. Thermal Stability; Compounds are stable as solids at temperatures greater than 500°C. Corrosiveness; Electrochemical corrosion rate studies have indicated that these compounds are significantly less corrosive than boric acid. Use of 3M"T"M Neutron Quench can lead to reduction in emergency shutdown pool size, reduce or remove the necessity for pool heating and heat tracing of lines, allow for more rapid introduction of the absorber in emergency situations or be used in other applications where significant neutron control is necessary. (author)

Power Aware Mobility Management of M2M for IoT Communications

Directory of Open Access Journals (Sweden)

Awais Ahmad

2015-01-01

Full Text Available Machine-to-Machine (M2M communications framework is evolving to sustain faster networks with the potential to connect millions of devices in the following years. M2M is one of the essential competences for implementing Internet of Things (IoT. Therefore, various organizations are now focusing on enhancing improvements into their standards to support M2M communications. Thus, Heterogeneous Mobile Ad Hoc Network (HetMANET can normally be considered appropriate for M2M challenges. These challenges incorporated when a mobile node (MN selects a target network in an energy efficient scanning for efficient handover. Therefore, to cope with these constraints, we proposed a vertical handover scheme for handover triggering and selection of an appropriate network. The proposed scheme is composed of two phases. Firstly, the MNs perform handover triggering based on the optimization of the Receive Signal Strength (RSS from an access point/base station (AP/BS. Secondly, the network selection process is performed by considering the cost and energy consumption of a particular application during handover. Moreover, if there are more networks available, then the MN selects the one provided with the highest quality of service (QoS. The decision regarding the selection of available networks is made on three metrics, that is, cost, energy, and data rate. Furthermore, the selection of an AP/BS of the selected network is made on five parameters: delay, jitter, Bit Error Rate (BER, communication cost, and response time. The numerical and experimental results are compared in the context of energy consumption by an MN, traffic management on an AP/BS, and QoS of the available networks. The proposed scheme efficiently optimizes the handoff related parameters, and it shows significant improvement in the existing models used for similar purpose.

Yrittäjyyden myytit ja möröt : case: Yritystakomo Oy, Oulu

OpenAIRE

Merentie, Elisa

2012-01-01

Tämän tutkimuksen tarkoituksena on selvittää mitkä yrittäjyyden myytit ja möröt vaikuttavat aloittavan yrittäjän uskallukseen aloittaa yrittäjänä. Ilmiötä tarkastelin tapausyrityksen, Yritystakomo Oy:n kautta. Ovatko vuosia kestäneet yrittäjyyden myytit, uskomukset aiheellisia ja sovellettavissa tämän päivän aloittelevan yrittäjän ajatusmaailmaan? Aihe on valittu, koska yrittäjyydestä ja sen edistämisestä puhutaan paljon, myös sen helppoudesta, mutta siitä huolimatta yrittäjyysidea jää usein...

High-resolution intracranial vessel wall MRI in an elderly asymptomatic population: comparison of 3T and 7T

Energy Technology Data Exchange (ETDEWEB)

Harteveld, Anita A.; Kolk, Anja G. van der; Dieleman, Nikki; Siero, Jeroen C.W.; Luijten, Peter R.; Zwanenburg, Jaco J.M.; Hendrikse, Jeroen [University Medical Center Utrecht, Department of Radiology, Postbox 85500, Utrecht (Netherlands); Worp, H.B. van der; Frijns, Catharina J.M. [University Medical Center Utrecht, Department of Neurology and Neurosurgery, Brain Center Rudolf Magnus, Utrecht (Netherlands); Kuijf, Hugo J. [University Medical Center Utrecht, Image Sciences Institute, Utrecht (Netherlands)

2017-04-15

Several intracranial vessel wall sequences have been described in recent literature, with either 3-T or 7-T magnetic resonance imaging (MRI). In the current study, we compared 3-T and 7-T MRI in visualising both the intracranial arterial vessel wall and vessel wall lesions. Twenty-one elderly asymptomatic volunteers were scanned by 3-T and 7-T MRI with an intracranial vessel wall sequence, both before and after contrast administration. Two raters scored image quality, and presence and characteristics of vessel wall lesions. Vessel wall visibility was equal or significantly better at 7 T for the studied arterial segments, even though there were more artefacts hampering assessment. The better visualisation of the vessel wall at 7 T was most prominent in the proximal anterior cerebral circulation and the posterior cerebral artery. In the studied elderly asymptomatic population, 48 vessel-wall lesions were identified at 3 T, of which 7 showed enhancement. At 7 T, 79 lesions were identified, of which 29 showed enhancement. Seventy-one percent of all 3-T lesions and 59 % of all 7-T lesions were also seen at the other field strength. Despite the large variability in detected lesions at both field strengths, we believe 7-T MRI has the highest potential to identify the total burden of intracranial vessel wall lesions. (orig.)

Agreement between manual relaxometry and semi-automated scanner-based multi-echo Dixon technique for measuring liver T2* in a pediatric and young adult population

International Nuclear Information System (INIS)

Serai, Suraj D.; Trout, Andrew T.; Dillman, Jonathan R.; Smith, Ethan A.

2018-01-01

Commercially available 3D multi-echo Dixon (mDixon) sequences provide parametric maps of liver T2*, obviating manual curve fitting that is often required with conventional gradient recalled echo (GRE)-based multi-echo relaxometry, potentially simplifying clinical work flow. The purpose of our study was to compare T2* values generated by a 3D mDixon sequence to values generated by GRE-based T2* relaxometry with manual curve fitting in a pediatric and young adult population. We reviewed clinical MRI exams performed at 1.5T for liver iron content estimation between February 2015 and June 2016 that included both mDixon and multi-echo GRE pulse sequences. We obtained mean T2* measurements based on each sequence by drawing regions of interest on each of four axial slices through the mid-liver. We compared mDixon-based and GRE-based T2* measurements using paired t-tests and assessed agreement using single-measure intra-class correlation coefficients and Bland-Altman difference plots. One hundred nine patients met inclusion criteria (site 1=82; site 2=27). Mean age was 12.4±5.8 years, and 42 subjects (39%) were female. There was no statistically significant difference in mean T2* values for the two sequences (pooled means: 11.7±11.0 [GRE] vs. 11.7±10.9 ms [mDixon]; P=0.93). There was excellent absolute agreement between sequences (intraclass correlation coefficient [ICC]=0.98 for patients at both sites, confidence interval [CI]: 0.97-0.98 with mean bias of 0.0 ms [-4.2 ms to +4.2 ms]). 3D mDixon is accurate for measuring liver T2* and can likely replace 2D GRE-based relaxometry. (orig.)

Agreement between manual relaxometry and semi-automated scanner-based multi-echo Dixon technique for measuring liver T2* in a pediatric and young adult population

Energy Technology Data Exchange (ETDEWEB)

Serai, Suraj D.; Trout, Andrew T.; Dillman, Jonathan R. [Cincinnati Children' s Hospital Medical Center, Department of Radiology, MLC 5031, Cincinnati, OH (United States); Smith, Ethan A. [Cincinnati Children' s Hospital Medical Center, Department of Radiology, MLC 5031, Cincinnati, OH (United States); University of Michigan Health System, Section of Pediatric Radiology, C.S. Mott Children' s Hospital, Department of Radiology, Ann Arbor, MI (United States)

2018-01-15

Commercially available 3D multi-echo Dixon (mDixon) sequences provide parametric maps of liver T2*, obviating manual curve fitting that is often required with conventional gradient recalled echo (GRE)-based multi-echo relaxometry, potentially simplifying clinical work flow. The purpose of our study was to compare T2* values generated by a 3D mDixon sequence to values generated by GRE-based T2* relaxometry with manual curve fitting in a pediatric and young adult population. We reviewed clinical MRI exams performed at 1.5T for liver iron content estimation between February 2015 and June 2016 that included both mDixon and multi-echo GRE pulse sequences. We obtained mean T2* measurements based on each sequence by drawing regions of interest on each of four axial slices through the mid-liver. We compared mDixon-based and GRE-based T2* measurements using paired t-tests and assessed agreement using single-measure intra-class correlation coefficients and Bland-Altman difference plots. One hundred nine patients met inclusion criteria (site 1=82; site 2=27). Mean age was 12.4±5.8 years, and 42 subjects (39%) were female. There was no statistically significant difference in mean T2* values for the two sequences (pooled means: 11.7±11.0 [GRE] vs. 11.7±10.9 ms [mDixon]; P=0.93). There was excellent absolute agreement between sequences (intraclass correlation coefficient [ICC]=0.98 for patients at both sites, confidence interval [CI]: 0.97-0.98 with mean bias of 0.0 ms [-4.2 ms to +4.2 ms]). 3D mDixon is accurate for measuring liver T2* and can likely replace 2D GRE-based relaxometry. (orig.)

Investigations on oil patches around 'M.T.Lajpatrai' Bombay harbour

Digital Repository Service at National Institute of Oceanography (India)

Kadam, A.N.; Zingde, M.D.

Samples from three oil patches observed around the burning tanker 'M. T. Lajpatrai' at Bombay Harbour were characterised using proton magnetic resonance spectroscopy (PMR). Two of them were found to be from used lubricating oils. As indicated by gas...

P-T-t metamorphic evolution of highly deformed metapelites from the Pinkie unit of western Svalbard using quartz-in-garnet barometry, trace element thermometry, P-T-X-M diagrams and monazite in-situ dating

Science.gov (United States)

Kośmińska, Karolina; Spear, Frank; Majka, Jarosław

2017-04-01

follows: ca. 590 C at 7.5 kbar for St-bearing metapelites, 570C at 8.5 kbar for St-Ky-bearing rocks, and 630 C at 10 kbar for Ky-bearing samples. The P-T-X-M diagrams calculated using the Fortran program GIBBS were used to examine how the garnet composition varies as a function of pressure and temperature. These diagrams suggest that a decrease in temperature and increase in pressure after garnet-I growth is needed to produce garnet-II. These results together with the QuiG results for garnet-II are consistent with late garnet nucleating and growing during mylonitization at 450-500 C and 10-12 kbar; thus an anti-clockwise P-T path is proposed for the Pinkie metapelites. Three monazite populations have been distinguished based on the textural observations and chemical investigations. The first population (high Th) gives an age of 373 Ma, which represents initial monazite growth during diagenesis or under low grade conditions. The second population (highest Y) yields an age of 359 Ma, and the third population (lower Y) gives an age of 355Ma. Monazite dating results coupled with the above P-T data provide constrain the amphibolite facies metamorphism to have occurred between 359-355 Ma. This study is supported by the Fulbright Junior Advanced Research Award (to KK), NCN project No 2013/11/N/ST10/00357 and AGH grant No 11.11.140.319.

ASSOCIATION OF ANGIOTENSINOGEN GENE M235T VARIANT IN PRE-ECLAMPSIA

International Nuclear Information System (INIS)

MAREI, E.S.; SAUDI, A.KH.; ANEES, L.M.; MOHAMMED, S.K.

2007-01-01

Pre-eclampsia (PE) is multisystem and multifactorial complication of pregnancy. The precise cause of pre-eclampsia has not been determined but mal adoption of the rennin and angiotensinogen (AGT) system may play a role. The products of genes involving the components of this system may be potential candidates for pre-eclampsia and hypertension related to pregnancy. This study was designed to determine whether the M235T variants of AGT gene were associated with the prevalence of pre-eclampsia and also to evaluate the role of plasma AGT in the development of the disease. Hence, developed, rapid and reliable PCR based assay was used to screen individuals for the M235T alleles. This assay was also used to genotype prospectively both recruited pregnant women with pre-eclampsia (n=24) and controls (n=11). Plasma AGT was determined by radioimmunoassay (RIA). The results of the PCR based assay revealed a significant association of 235T allele with the prevalence of all pre-eclamptic patients, Chi-square (x2) = 3.714, P 0.05). Also, pre-eclamptic women exhibited significant higher levels of plasma AGT (52.15 ± 1.63) versus controls (44.76 ± 4.6) with P value < 0.001. This gave clear evidence that T235T allele and plasma AGT contributed to the development of pre-eclampsia with pregnancy and correlated with severity of the disease

M to T Rearrangement: An Approach to Correct Webbed Neck Deformity

Directory of Open Access Journals (Sweden)

Ananth S. Murthy

2014-01-01

Full Text Available For the Noonan syndrome patient, the most concerning physical defect is often congenital webbing of the neck or pterygium colli. We present a patient with pterygium colli and a low and laterally displaced nuchal hairline. Since its description, various surgical approaches have been implemented to correct the deformity. Previously reported posterior and lateral approaches have notable disadvantages with regard to hairline displacement and recurrence. In order to address these disadvantages, a new surgical technique was used on this patient. We have termed this technique an M to T rearrangement. Using a lateral approach, the M and T incisions are made and the trapezial fascial web is directly visualized and able to be completely excised. This prevents the recurrence seen with the use of posterior techniques. Inferolateral displacement of hair-bearing skin can be removed with resection of the superior intervening triangle and improves the appearance of the low nuchal hairline. The excision of excess skin along with the zig-zag closure also prevents postoperative scar contraction and recurrence. An important effect of this technique is the prevention of anterior displacement of hair bearing skin. M to T rearrangement is an effective technique for the correction of webbed neck deformities seen in Noonan and Turner syndromes.

The influence of physical activity on body image in people with and without acquired mobility disability [Vliv pohybové aktivity na subjektivní vnímání těla u osob se získaným tělesným postižením a bez něj

Directory of Open Access Journals (Sweden)

Ziya Koruç

2011-12-01

Full Text Available BACKGROUND: Body image in people with physical disability is important, but it has received little attention in the research literature. OBJECTIVE: The aim of the study was to determine whether differences exist between adolescents with acquired mobility disability (AMD and those without AMD regarding body image, and whether physical activity influences these differences. METHODS: Fifty-eight adolescents, aged 16 to 18 years, participated in this study. Half the participants had some form of AMD while the other half were healthy. Body image was evaluated with the Multidimensional Body-Self Relations Questionnaire (MBSRQ before and after 6 weeks of playing darts. A two-way ANOVA was used to analyse the results. RESULTS: At the end of the study, the healthy adolescents scored significantly higher than the AMD group on the subscales of fitness perception, orientation and overall health perception. No interaction was found between disability and exercise on any subscales of the MBSRQ. CONCLUSIONS: The results of this study demonstrate that people with AMD evaluate their health and fitness levels as being lower than healthy adolescents and that they are less concerned with fitness as compared with healthy adolescents. Six weeks of playing darts as a physical activity had no effect on improving the self-perceptions of the AMD group.[VÝCHODISKA: Subjektivní vnímání těla u lidí s tělesným postižením je důležitý aspekt, v odborné literatuře mu však nebylo věnováno příliš pozornosti. CÍLE: Cílem studie bylo stanovit, zda se vyskytují rozdíly mezi adolescenty se získaným tělesným postižením (AMD a adolescenty bez AMD, co se týče subjektivního vnímání těla, a zda tyto rozdíly ovlivňuje pohybová aktivita. METODIKA: Této studie se zúčastnilo padesát osm adolescentů ve věku mezi 16 a 18 lety. Polovina účastníků měla některou z forem AMD, zatímco druhá polovina byla zdravá. Subjektivní vnímání t

Electric field gradient at the Nb3M(M = Al, In, Si, Ge, Sn) and T3Al (T = Ti, Zr, Hf, V, Nb, Ta) alloys by perturbed angular correlation method

International Nuclear Information System (INIS)

Junqueira, Astrogildo de Carvalho

1999-01-01

The electric field gradient (efg) at the Nb site in the intermetallic compounds Nb 3 M (M = Al, Si, Ge, Sn) and at the T site in the intermetallic compounds T 3 Al (T = Ti, Zr, Hf, V, Nb, Ta) was measured by Perturbed Angular Correlation (PAC) method using the well known gamma-gamma cascade of 133-482 keV in 181 Ta from the β - decay of 181 Hf. The compounds were prepared by arc melting the constituent elements under argon atmosphere along with radioactive 181 Hf substituting approximately 0.1 atomic percent of Nb and T elements. The PAC measurements were carried out at 295 K for all compounds and the efg was obtained for each alloy. The results for the efg in the T 3 Al compounds showed a strong correlation with the number of conduction electrons, while for the Nbs M compounds the efg behavior is influenced mainly by the p electrons of the M elements. The so-called universal correlation between the electronic and lattice contribution for the efg in metals was not verified in this work for all studied compounds. Measurements of the quadrupole frequency in the range of 100 to 1210 K for the Nb 3 Al compound showed a linear behaviour with the temperature. Superconducting properties of this alloys may probably be related with this observed behaviour. The efg results are compared to those reported for other binary alloys and discussed with the help of ab-initio methods. (author)

A consensus linkage map of lentil based on DArT markers from three RIL mapping populations.

Directory of Open Access Journals (Sweden)

Duygu Ates

Full Text Available Lentil (Lens culinaris ssp. culinaris Medikus is a diploid (2n = 2x = 14, self-pollinating grain legume with a haploid genome size of about 4 Gbp and is grown throughout the world with current annual production of 4.9 million tonnes.A consensus map of lentil (Lens culinaris ssp. culinaris Medikus was constructed using three different lentils recombinant inbred line (RIL populations, including "CDC Redberry" x "ILL7502" (LR8, "ILL8006" x "CDC Milestone" (LR11 and "PI320937" x "Eston" (LR39.The lentil consensus map was composed of 9,793 DArT markers, covered a total of 977.47 cM with an average distance of 0.10 cM between adjacent markers and constructed 7 linkage groups representing 7 chromosomes of the lentil genome. The consensus map had no gap larger than 12.67 cM and only 5 gaps were found to be between 12.67 cM and 6.0 cM (on LG3 and LG4. The localization of the SNP markers on the lentil consensus map were in general consistent with their localization on the three individual genetic linkage maps and the lentil consensus map has longer map length, higher marker density and shorter average distance between the adjacent markers compared to the component linkage maps.This high-density consensus map could provide insight into the lentil genome. The consensus map could also help to construct a physical map using a Bacterial Artificial Chromosome library and map based cloning studies. Sequence information of DArT may help localization of orientation scaffolds from Next Generation Sequencing data.

A consensus linkage map of lentil based on DArT markers from three RIL mapping populations.

Science.gov (United States)

Ates, Duygu; Aldemir, Secil; Alsaleh, Ahmad; Erdogmus, Semih; Nemli, Seda; Kahriman, Abdullah; Ozkan, Hakan; Vandenberg, Albert; Tanyolac, Bahattin

2018-01-01

Lentil (Lens culinaris ssp. culinaris Medikus) is a diploid (2n = 2x = 14), self-pollinating grain legume with a haploid genome size of about 4 Gbp and is grown throughout the world with current annual production of 4.9 million tonnes. A consensus map of lentil (Lens culinaris ssp. culinaris Medikus) was constructed using three different lentils recombinant inbred line (RIL) populations, including "CDC Redberry" x "ILL7502" (LR8), "ILL8006" x "CDC Milestone" (LR11) and "PI320937" x "Eston" (LR39). The lentil consensus map was composed of 9,793 DArT markers, covered a total of 977.47 cM with an average distance of 0.10 cM between adjacent markers and constructed 7 linkage groups representing 7 chromosomes of the lentil genome. The consensus map had no gap larger than 12.67 cM and only 5 gaps were found to be between 12.67 cM and 6.0 cM (on LG3 and LG4). The localization of the SNP markers on the lentil consensus map were in general consistent with their localization on the three individual genetic linkage maps and the lentil consensus map has longer map length, higher marker density and shorter average distance between the adjacent markers compared to the component linkage maps. This high-density consensus map could provide insight into the lentil genome. The consensus map could also help to construct a physical map using a Bacterial Artificial Chromosome library and map based cloning studies. Sequence information of DArT may help localization of orientation scaffolds from Next Generation Sequencing data.

Methylation of arsenic by recombinant human wild-type arsenic (+ 3 oxidation state) methyltransferase and its methionine 287 threonine (M287T) polymorph: Role of glutathione

Energy Technology Data Exchange (ETDEWEB)

Ding, Lan; Saunders, R. Jesse; Drobná, Zuzana; Walton, Felecia S.; Xun, Pencheng [Department of Nutrition, Gillings School of Global Public Health, 2302 MHRC, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7461 (United States); Thomas, David J. [Pharmacokinetics Branch, Mail Drop B 143-01, Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 109 Alexander Drive Research Triangle Park, NC 27711 (United States); Stýblo, Miroslav, E-mail: styblo@med.unc.edu [Department of Nutrition, Gillings School of Global Public Health, 2302 MHRC, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7461 (United States)

2012-10-01

Arsenic (+ 3 oxidation state) methyltransferase (AS3MT) is the key enzyme in the pathway for methylation of arsenicals. A common polymorphism in the AS3MT gene that replaces a threonyl residue in position 287 with a methionyl residue (AS3MT/M287T) occurs at a frequency of about 10% among populations worldwide. Here, we compared catalytic properties of recombinant human wild-type (wt) AS3MT and AS3MT/M287T in reaction mixtures containing S-adenosylmethionine, arsenite (iAs{sup III}) or methylarsonous acid (MAs{sup III}) as substrates and endogenous or synthetic reductants, including glutathione (GSH), a thioredoxin reductase (TR)/thioredoxin (Trx)/NADPH reducing system, or tris (2-carboxyethyl) phosphine hydrochloride (TCEP). With either TR/Trx/NADPH or TCEP, wtAS3MT or AS3MT/M287T catalyzed conversion of iAs{sup III} to MAs{sup III}, methylarsonic acid (MAs{sup V}), dimethylarsinous acid (DMAs{sup III}), and dimethylarsinic acid (DMAs{sup V}); MAs{sup III} was converted to DMAs{sup III} and DMAs{sup V}. Although neither enzyme required GSH to support methylation of iAs{sup III} or MAs{sup III}, addition of 1 mM GSH decreased K{sub m} and increased V{sub max} estimates for either substrate in reaction mixtures containing TR/Trx/NADPH. Without GSH, V{sub max} and K{sub m} values were significantly lower for AS3MT/M287T than for wtAS3MT. In the presence of 1 mM GSH, significantly more DMAs{sup III} was produced from iAs{sup III} in reactions catalyzed by the M287T variant than in wtAS3MT-catalyzed reactions. Thus, 1 mM GSH modulates AS3MT activity, increasing both methylation rates and yield of DMAs{sup III}. AS3MT genotype exemplified by differences in regulation of wtAS3MT and AS3MT/M287T-catalyzed reactions by GSH may contribute to differences in the phenotype for arsenic methylation and, ultimately, to differences in the disease susceptibility in individuals chronically exposed to inorganic arsenic. -- Highlights: ► Human AS3MT and AS3MT(M287T) require a dithiol

Methylation of arsenic by recombinant human wild-type arsenic (+ 3 oxidation state) methyltransferase and its methionine 287 threonine (M287T) polymorph: Role of glutathione

International Nuclear Information System (INIS)

Ding, Lan; Saunders, R. Jesse; Drobná, Zuzana; Walton, Felecia S.; Xun, Pencheng; Thomas, David J.; Stýblo, Miroslav

2012-01-01

Arsenic (+ 3 oxidation state) methyltransferase (AS3MT) is the key enzyme in the pathway for methylation of arsenicals. A common polymorphism in the AS3MT gene that replaces a threonyl residue in position 287 with a methionyl residue (AS3MT/M287T) occurs at a frequency of about 10% among populations worldwide. Here, we compared catalytic properties of recombinant human wild-type (wt) AS3MT and AS3MT/M287T in reaction mixtures containing S-adenosylmethionine, arsenite (iAs III ) or methylarsonous acid (MAs III ) as substrates and endogenous or synthetic reductants, including glutathione (GSH), a thioredoxin reductase (TR)/thioredoxin (Trx)/NADPH reducing system, or tris (2-carboxyethyl) phosphine hydrochloride (TCEP). With either TR/Trx/NADPH or TCEP, wtAS3MT or AS3MT/M287T catalyzed conversion of iAs III to MAs III , methylarsonic acid (MAs V ), dimethylarsinous acid (DMAs III ), and dimethylarsinic acid (DMAs V ); MAs III was converted to DMAs III and DMAs V . Although neither enzyme required GSH to support methylation of iAs III or MAs III , addition of 1 mM GSH decreased K m and increased V max estimates for either substrate in reaction mixtures containing TR/Trx/NADPH. Without GSH, V max and K m values were significantly lower for AS3MT/M287T than for wtAS3MT. In the presence of 1 mM GSH, significantly more DMAs III was produced from iAs III in reactions catalyzed by the M287T variant than in wtAS3MT-catalyzed reactions. Thus, 1 mM GSH modulates AS3MT activity, increasing both methylation rates and yield of DMAs III . AS3MT genotype exemplified by differences in regulation of wtAS3MT and AS3MT/M287T-catalyzed reactions by GSH may contribute to differences in the phenotype for arsenic methylation and, ultimately, to differences in the disease susceptibility in individuals chronically exposed to inorganic arsenic. -- Highlights: ► Human AS3MT and AS3MT(M287T) require a dithiol reductant for optimal activity. ► Both enzymes methylate arsenite to tri- and

The S.M.A.R.T. Strategy to Recruiting and Retaining High School Coaches

Science.gov (United States)

Lubisco, Robyn; Birren, Genevieve F. E.

2017-01-01

This article discusses the S.M.A.R.T. strategy for recruiting and retaining quality high school coaches. S.M.A.R.T. stands for Scouting, Mentoring and Coaching, Appreciation, Rating, and Time. Scouting addresses how one goes about locating and hiring quality coaches. Mentoring and Coaching addresses how to develop the coach within the specific…

A colitogenic memory CD4+ T cell population mediates gastrointestinal graft-versus-host disease

Science.gov (United States)

Zhou, Vivian; Agle, Kimberle; Chen, Xiao; Beres, Amy; Komorowski, Richard; Belle, Ludovic; Taylor, Carolyn; Zhu, Fenlu; Haribhai, Dipica; Williams, Calvin B.; Verbsky, James; Blumenschein, Wendy; Sadekova, Svetlana; Bowman, Eddie; Ballantyne, Christie; Weaver, Casey; Serody, David A.; Vincent, Benjamin; Serody, Jonathan; Cua, Daniel J.; Drobyski, William R.

2016-01-01

Damage to the gastrointestinal tract is a major cause of morbidity and mortality in graft-versus-host disease (GVHD) and is attributable to T cell–mediated inflammation. In this work, we identified a unique CD4+ T cell population that constitutively expresses the β2 integrin CD11c and displays a biased central memory phenotype and memory T cell transcriptional profile, innate-like properties, and increased expression of the gut-homing molecules α4β7 and CCR9. Using several complementary murine GVHD models, we determined that adoptive transfer and early accumulation of β2 integrin–expressing CD4+ T cells in the gastrointestinal tract initiated Th1-mediated proinflammatory cytokine production, augmented pathological damage in the colon, and increased mortality. The pathogenic effect of this CD4+ T cell population critically depended on coexpression of the IL-23 receptor, which was required for maximal inflammatory effects. Non–Foxp3-expressing CD4+ T cells produced IL-10, which regulated colonic inflammation and attenuated lethality in the absence of functional CD4+Foxp3+ T cells. Thus, the coordinate expression of CD11c and the IL-23 receptor defines an IL-10–regulated, colitogenic memory CD4+ T cell subset that is poised to initiate inflammation when there is loss of tolerance and breakdown of mucosal barriers. PMID:27500496

Desenvolvimento do teste de sensibilidade tátil da mão

OpenAIRE

Leonardo Penteado Nascimento

2014-01-01

Contextualização: Os testes de sensibilidade tátil da mão existentes não permitem considerar diferentes receptores, detectar variações na faixa de normalidade ou caracterizar o processo de desenvolvimento e envelhecimento. Objetivo: Elaborar um conjunto de atividades-testes para avaliar sensibilidade tátil da mão que forneça escore e tempo de execução das tarefas, com rotina de aplicação e coleta de dados detalhada em manual. Método: Para elaboração do teste foi realizada revisão da literatur...

Preferences of AAA/AAG codon recognition by modified nucleosides, τm5s2U34 and t6A37 present in tRNALys.

Science.gov (United States)

Sonawane, Kailas D; Kamble, Asmita S; Fandilolu, Prayagraj M

2017-12-27

Deficiency of 5-taurinomethyl-2-thiouridine, τm 5 s 2 U at the 34th 'wobble' position in tRNA Lys causes MERRF (Myoclonic Epilepsy with Ragged Red Fibers), a neuromuscular disease. This modified nucleoside of mt tRNA Lys , recognizes AAA/AAG codons during protein biosynthesis process. Its preference to identify cognate codons has not been studied at the atomic level. Hence, multiple MD simulations of various molecular models of anticodon stem loop (ASL) of mt tRNA Lys in presence and absence of τm 5 s 2 U 34 and N 6 -threonylcarbamoyl adenosine (t 6 A 37 ) along with AAA and AAG codons have been accomplished. Additional four MD simulations of multiple ASL mt tRNA Lys models in the context of ribosomal A-site residues have also been performed to investigate the role of A-site in recognition of AAA/AAG codons. MD simulation results show that, ASL models in presence of τm 5 s 2 U 34 and t 6 A 37 with codons AAA/AAG are more stable than the ASL lacking these modified bases. MD trajectories suggest that τm 5 s 2 U recognizes the codons initially by 'wobble' hydrogen bonding interactions, and then tRNA Lys might leave the explicit codon by a novel 'single' hydrogen bonding interaction in order to run the protein biosynthesis process smoothly. We propose this model as the 'Foot-Step Model' for codon recognition, in which the single hydrogen bond plays a crucial role. MD simulation results suggest that, tRNA Lys with τm 5 s 2 U and t 6 A recognizes AAA codon more preferably than AAG. Thus, these results reveal the consequences of τm 5 s 2 U and t 6 A in recognition of AAA/AAG codons in mitochondrial disease, MERRF.

Diabetter"T"M Reduces Post Meal Hyperglycemia Via Enhancement of Glucose Uptake Into Adipocytes and Muscles Cells

International Nuclear Information System (INIS)

Zainah Adam; Mohd Hishamudin Mohd Jinal; Alqarni Bader Ayed; Shafii Khamis

2014-01-01

There are lots of herbal products for diabetes mellitus treatment available in local market. Most of these products are not standardized and lack of efficacy and safety data. DiaBetter"T"M is one of the herbal products that have been used for diabetes treatment. This study was carried out to determine the efficacy of DiaBetter"T"M in reducing hyperglycemia and to elucidate the mechanisms by which hyperglycemia is reduced. The results showed that DiaBetter"T"M significantly reduced post meal hyperglycemia in normal and diabetic rats, and improved glucose tolerance activity in diabetic rats particularly after 4 and 6 hours of administration. Antihyperglycemic mechanisms elucidation revealed that the DiaBetter"T"M significantly enhanced insulin-stimulated glucose uptake into adipocytes and muscle cells, with the highest magnitude of enhancement were 1.54 fold (p<0.01) and 1.46 fold (p<0.001), respectively. Molecular mechanisms that responsible for this enhancement were the increment of insulin sensitivity at cells membrane. Cytotoxic evaluation was also done and confirmed that DiaBetter"T"M was toxicologically safe against muscle and adipocytes cells. In conclusion, post-meal antihyperglycemic and glucose tolerance activity of DiaBetter"T"M was mediated through the enhancement of glucose uptake into adipocytes and muscle cells. Insulin sensitizing activity showed by DiaBetter"T"M suggests that this product has the potential to ameliorate insulin resistance condition. Therefore, it is suggested that the DiaBetter"T"M can be used as dietary adjunct for the management of type 2 diabetes mellitus which related to insulin resistance. (Author)

"Don't know" responses to risk perception measures: implications for underserved populations.

Science.gov (United States)

Waters, Erika A; Hay, Jennifer L; Orom, Heather; Kiviniemi, Marc T; Drake, Bettina F

2013-02-01

Risk perceptions are legitimate targets for behavioral interventions because they can motivate medical decisions and health behaviors. However, some survey respondents may not know (or may not indicate) their risk perceptions. The scope of "don't know" (DK) responding is unknown. Examine the prevalence and correlates of responding DK to items assessing perceived risk of colorectal cancer. Two nationally representative, population-based, cross-sectional surveys (2005 National Health Interview Survey [NHIS]; 2005 Health Information National Trends Survey [HINTS]), and one primary care clinic-based survey comprised of individuals from low-income communities. Analyses included 31,202 (NHIS), 1,937 (HINTS), and 769 (clinic) individuals. Five items assessed perceived risk of colorectal cancer. Four of the items differed in format and/or response scale: comparative risk (NHIS, HINTS); absolute risk (HINTS, clinic), and "likelihood" and "chance" response scales (clinic). Only the clinic-based survey included an explicit DK response option. "Don't know" responding was 6.9% (NHIS), 7.5% (HINTS-comparative), and 8.7% (HINTS-absolute). "Don't know" responding was 49.1% and 69.3% for the "chance" and "likely" response options (clinic). Correlates of DK responding were characteristics generally associated with disparities (e.g., low education), but the pattern of results varied among samples, question formats, and response scales. The surveys were developed independently and employed different methodologies and items. Consequently, the results were not directly comparable. There may be multiple explanations for differences in the magnitude and characteristics of DK responding. "Don't know" responding is more prevalent in populations affected by health disparities. Either not assessing or not analyzing DK responses could further disenfranchise these populations and negatively affect the validity of research and the efficacy of interventions seeking to eliminate health disparities.

Structural insights into drug development strategy targeting EGFR T790M/C797S.

Science.gov (United States)

Zhu, Su-Jie; Zhao, Peng; Yang, Jiao; Ma, Rui; Yan, Xiao-E; Yang, Sheng-Yong; Yang, Jing-Wen; Yun, Cai-Hong

2018-03-02

Treatment of non-small-cell lung cancers (NSCLCs) harboring primary EGFR oncogenic mutations such as L858R and exon 19 deletion delE746_A750 (Del-19) using gefitinib/erlotinib ultimately fails due to the emergence of T790M mutation. Though WZ4002/CO-1686/AZD9291 are effective in overcoming EGFR T790M by targeting Cys797 via covalent bonding, their efficacy is again limited due to the emergence of C797S mutation. New agents effectively inhibiting EGFR T790M without covalent linkage through Cys 797 may solve this problem. We presented here crystal structures of EGFR activating/drug-resistant mutants in complex with a panel of reversible inhibitors along with mutagenesis and enzyme kinetic data. These data revealed a previously un-described hydrophobic clamp structure in the EGFR kinase which may be exploited to facilitate development of next generation drugs targeting EGFR T790M with or without concomitant C797S. Interestingly, mutations in the hydrophobic clamp that hinder drug binding often also weaken ATP binding and/or abolish kinase activity, thus do not readily result in resistance to the drugs.

Estrogen receptor (α and β) but not androgen receptor expression is correlated with recurrence, progression and survival in post prostatectomy T3N0M0 locally advanced prostate cancer in an urban Greek population.

Science.gov (United States)

Megas, Georgios; Chrisofos, Michael; Anastasiou, Ioannis; Tsitlidou, Aida; Choreftaki, Theodosia; Deliveliotis, Charalampos

2015-01-01

The objective of this study was to evaluate the expression of estrogen receptors (ER(α) and ER(β)) and androgen receptors (ARs) as prognostic factors for biochemical recurrence, disease progression and survival in patients with pT3N0M0 prostate cancer (PCa) in an urban Greek population. A total of 100 consecutive patients with pT3N0M0 PCa treated with radical prostatectomy participated in the study. The mean age and follow-up were 64.2 and 6 years, respectively. The HSCORE was used for semi-quantitative analysis of the immunoreactivity of the receptors. The prognostic value of the ER(α) and ER(β) and AR was assessed in terms of recurrence, progression, and survival. AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis. A univariate Cox regression analysis showed that ER(α) positive staining was significantly associated with a greater hazard for all outcomes. Increased ER(β) staining was significantly associated with a lower hazard for all outcomes in the univariate analysis. When both ER HSCORES were used for the analysis, it was found that patients with high ER(α) or low ER(β) HSCORES compared with patients with negatively stained ER(α) and >1.7 hSCORE ER(β) had 6.03, 10.93, and 10.53 times greater hazard for biochemical disease recurrence, progression of disease and death, respectively. Multiple Cox proportional hazard analyses showed that the age, preoperative prostate specific antigen, Gleason score and ERs were independent predictors of all outcomes. ER expression is an important prognosticator after radical prostatectomy in patients with pT3N0M0 PCa. By contrast, AR expression has limited prognostic value.

Estrogen receptor (α and β but not androgen receptor expression is correlated with recurrence, progression and survival in post prostatectomy T3N0M0 locally advanced prostate cancer in an urban Greek population

Directory of Open Access Journals (Sweden)

Georgios Megas

2015-02-01

Full Text Available The objective of this study was to evaluate the expression of estrogen receptors (ER(α and ER(β and androgen receptors (ARs as prognostic factors for biochemical recurrence, disease progression and survival in patients with pT3N0M0 prostate cancer (PCa in an urban Greek population. A total of 100 consecutive patients with pT3N0M0 PCa treated with radical prostatectomy participated in the study. The mean age and follow-up were 64.2 and 6 years, respectively. The HSCORE was used for semi-quantitative analysis of the immunoreactivity of the receptors. The prognostic value of the ER(α and ER(β and AR was assessed in terms of recurrence, progression, and survival. AR expression was not associated with any of the above parameters; however, both ERs correlated with the prognosis. A univariate Cox regression analysis showed that ER(α positive staining was significantly associated with a greater hazard for all outcomes. Increased ER(β staining was significantly associated with a lower hazard for all outcomes in the univariate analysis. When both ER HSCORES were used for the analysis, it was found that patients with high ER(α or low ER(β HSCORES compared with patients with negatively stained ER(α and >1.7 hSCORE ER(β had 6.03, 10.93, and 10.53 times greater hazard for biochemical disease recurrence, progression of disease and death, respectively. Multiple Cox proportional hazard analyses showed that the age, preoperative prostate specific antigen, Gleason score and ERs were independent predictors of all outcomes. ER expression is an important prognosticator after radical prostatectomy in patients with pT3N0M0 PCa. By contrast, AR expression has limited prognostic value.

Anti-IL-2 treatment impairs the expansion of T(reg cell population during acute malaria and enhances the Th1 cell response at the chronic disease.

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Cláudia A Zago

Full Text Available Plasmodium chabaudi infection induces a rapid and intense splenic CD4(+ T cell response that contributes to both disease pathogenesis and the control of acute parasitemia. The subsequent development of clinical immunity to disease occurs concomitantly with the persistence of low levels of chronic parasitemia. The suppressive activity of regulatory T (T(reg cells has been implicated in both development of clinical immunity and parasite persistence. To evaluate whether IL-2 is required to induce and to sustain the suppressive activity of T(reg cells in malaria, we examined in detail the effects of anti-IL-2 treatment with JES6-1 monoclonal antibody (mAb on the splenic CD4(+ T cell response during acute and chronic P. chabaudi AS infection in C57BL/6 mice. JES6-1 treatment on days 0, 2 and 4 of infection partially inhibits the expansion of the CD4(+CD25(+Foxp3(+ cell population during acute malaria. Despite the concomitant secretion of IL-2 and expression of high affinity IL-2 receptor by large CD4(+ T cells, JES6-1 treatment does not impair effector CD4(+ T cell activation and IFN-γ production. However, at the chronic phase of the disease, an enhancement of cellular and humoral responses occurs in JES6-1-treated mice, with increased production of TNF-α and parasite-specific IgG2a antibodies. Furthermore, JES6-1 mAb completely blocked the in vitro proliferation of CD4(+ T cells from non-treated chronic mice, while it further increased the response of CD4(+ T cells from JES6-1-treated chronic mice. We conclude that JES6-1 treatment impairs the expansion of T(reg cell population during early P. chabaudi malaria and enhances the Th1 cell response in the late phase of the disease.

The planetary nebulae population in the nuclear regions of M31: the SAURON view

Science.gov (United States)

Pastorello, Nicola; Sarzi, Marc; Cappellari, Michele; Emsellem, Eric; Mamon, Gary A.; Bacon, Roland; Davies, Roger L.; de Zeeuw, P. Tim

2013-04-01

The study of extragalactic planetary nebulae (PNe) in the optical regions of galaxies, where the properties of their stellar population can be best characterized, is a promising ground to better understand the late evolution of stars across different galactic environments. Following a first study of the central regions of M32 that illustrated the power of integral field spectroscopy (IFS) in detecting and measuring the [O III] λ5007 emission of PNe against a strong stellar background, we turn to the very nuclear PN population of M31, within ˜80 pc of its centre. We show that PNe can also be found in the presence of emission from diffuse gas, as commonly observed in early-type galaxies and in the bulge of spirals, and further illustrate the excellent sensitivity of IFS in detecting extragalactic PNe through a comparison with narrow-band images obtained with the Hubble Space Telescope. Contrary to the case of the central regions of M32, the nuclear PNe population of M31 is only marginally consistent with the generally adopted form of the PNe luminosity function (PNLF). In particular, this is due to a lack of PNe with absolute magnitude M5007 brighter than -3, which would only result from a rather unfortunate draw from such a model PNLF. The nuclear stellar population of M31 is quite different from that of the central regions of M32, which is characterized in particular by a larger metallicity and a remarkable ultraviolet (UV) upturn. We suggest that the observed lack of bright PNe in the nuclear regions of M31 is due to a horizontal-branch population that is more tilted towards less massive and hotter He-burning stars, so that its progeny consists mostly of UV-bright stars that fail to climb back up the asymptotic giant branch (AGB) and only a few, if any, bright PNe powered by central post-AGB stars. These results are also consistent with recent reports on a dearth of bright post-AGB stars towards the nucleus of M31, and lend further support to the idea that the

Integrētā mārketinga komunikācija zīmola "FRUTEVER" virzīšanā

OpenAIRE

Zaharova, Sofja

2015-01-01

Bakalaura darba temats ir “Integrētā mārketinga komunikācija zīmola “FRUTEVER” virzīšanā”. Darba mērķis ir, pamatojoties uz teorētiskajām atziņām, izpētīt zīmola “FRUTEVER” mērķauditoriju, lai noskaidrotu, kā pozicionēt zīmolu un caur kuriem komunikācijas kanāliem uzrunāt potenciālos klientus. Darba teorētiskajā daļā ir apskatīta teorija par zīmola jēdzienu, tā izstrādes un pozicionēšanas pamatnosacījumiem, kā arī par mārketinga komunikāciju, īpaši pievēršoties integrētās mārketinga komuni...

Trumpi revolutsiooniline dilemma / Harold James ; inglise keelest tõlkinud M. V.

Index Scriptorium Estoniae

James, Harold

2017-01-01

Vene revolutsiooni tänavune sajas aastapäev langeb kokku Trumpi revolutsiooniga USA-s, mis omakorda järgnes Brexiti revolutsioonile Ühendkuningriigis. Tänased mässajad peaksid arvesse võtma ajaloo õppetunde

Tööotsija töötuks tunnistamise ning töötu abiraha määramise ja maksmise ajutise juhendi kinnitamise kohta

Index Scriptorium Estoniae

1991-01-01

Kehtib 1. aprillist 1991. Lisatud ajutine juhend: töö vahendamine tööotsijale, töötule, töötu abiraha määramine ja maksmine Lisatud: töötu abiraha arvutamine. Muudatus: RT I 1992 nr. 27 art. 362

The m.3291T>C mt-tRNALeu(UUR) mutation is definitely pathogenic and causes multisystem mitochondrial disease

Science.gov (United States)

Yarham, John W.; Blakely, Emma L.; Alston, Charlotte L.; Roberts, Mark E.; Ealing, John; Pal, Piyali; Turnbull, Douglass M.; McFarland, Robert; Taylor, Robert W.

2013-01-01

Mitochondrial tRNA point mutations are important causes of human disease, and have been associated with a diverse range of clinical phenotypes. Definitively proving the pathogenicity of any given mt-tRNA mutation requires combined molecular, genetic and functional studies. Subsequent evaluation of the mutation using a pathogenicity scoring system is often very helpful in concluding whether or not the mutation is causing disease. Despite several independent reports linking the m.3291T>C mutation to disease in humans, albeit in association with several different phenotypes, its pathogenicity remains controversial. A lack of conclusive functional evidence and an over-emphasis on the poor evolutionary conservation of the affected nucleotide have contributed to this controversy. Here we describe an adult patient who presented with deafness and lipomas and evidence of mitochondrial abnormalities in his muscle biopsy, who harbours the m.3291T > C mutation, providing conclusive evidence of pathogenicity through analysis of mutation segregation with cytochrome c oxidase (COX) deficiency in single muscle fibres, underlining the importance of performing functional studies when assessing pathogenicity. PMID:23273904

Varan määritelmä ja varoihin liittyvät laskentasäännöt IPSAS-standardeissa

OpenAIRE

LUOMA, MIKKO

2008-01-01

Tässä tutkimuksessa luodaan käsiteanalyyttinen katsaus IPSAS-standardien varan määritelmään. Tutkimuksessa arvioidaan IPSAS-standardien varan määritelmää ja siinä esitettyjä lausumia sekä peilataan niitä julkisyhteisön varojen ominaispiirteisiin. Lisäksi tutkimuksessa arvioidaan miten annettu määritelmä vaikuttaa IPSAS-standardien lähtökohtiin ja laskentaperiaatteisiin. Tutkimuksen perusteella voidaan todeta, että varan määritelmä on jokseenkin problemaattinen. Keskeisimmät ongelmat määr...

Plasmodium chabaudi in mice. Adoptive transfer of immunity with enriched populations of spleen T and B lymphocytes

International Nuclear Information System (INIS)

McDonald, V.; Phillips, R.S.

1978-01-01

Thymectomized NIH and C57BL mice were more susceptible to Plasmodium chabaudi than controls, indicating a role for T cells in acquired immunity to the parasite. Enriched populations of T and B cells were prepared from the spleens of immune mice using nylon-wool columns, and were adoptively transferred to syngeneic non-irradiated mice or mice irradiated with 600 or 800 rad. Some immunity could usually be transferred with immune T, B and glass-wool (g.w.) filtered spleen cell populations. In the heavily irradiated mice g.w. filtered immune spleen cells gave the best protection and the immune T cells the least. Preliminary attempts to show synergistic activity between immune T and B cells in irradiated mice were not successful. (author)

Initial phase of adaptation of Memory Alteration Test (M@T) in a Portuguese sample.

Science.gov (United States)

Sousa, Mónica; Pereira, Anabela; Costa, Rui; Rami, Lorena

2015-01-01

The Memory Alteration Test is a screening test able to discriminate the amnestic mild cognitive impairment (A-MCI) and mild Alzheimer disease from subjective memory complain group (SMC-G). The aim of this study was to analyze the European Portuguese experimental version of the Memory Alteration Test (M@T-PT). Were described the successive stages of the translation and adaptation procedure used to develop the M@T-PT. The psychometric properties were explored using principal component analysis (PCA) with varimax rotation, internal consistence, convergent validity and receiver operating characteristics (ROC) curves. Chi-squared, ANOVAs and Pearson's correlation were also analyzed. A total of 330 people with an age greater or equal to 54 years old participated. According to their cognitive state they were classified as normal controls (n=28), SMC-G (n=81) or A-MCI (n=221). The PCA for the M@T-PT indicated that the total variance explained by three components was 48.07%. A high internal consistent was obtained (α=0.93). Convergent validity was verified using M@T-PT and Mini-Mental State Examination (r=0.83, psound instrument for use in older people in Portugal. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Group I mGlu receptor stimulation inhibits activation-induced cell death of human T lymphocytes

Science.gov (United States)

Chiocchetti, Annalisa; Miglio, Gianluca; Mesturini, Riccardo; Varsaldi, Federica; Mocellin, Marco; Orilieri, Elisabetta; Dianzani, Chiara; Fantozzi, Roberto; Dianzani, Umberto; Lombardi, Grazia

2006-01-01

The effects of L-glutamate on activation-induced cell death (AICD) of human activated (1 μg ml−1 phytohemagglutinin plus 2 U ml−1 interleukin-2; 8 days) T lymphocytes were studied by measuring anti-CD3 monoclonal antibody (10 μg ml−1; 18 h)-induced cell apoptosis (Annexin V and propidium iodide staining). L-Glutamate (1 × 10−8–1 × 10−4 M) significantly (P⩽0.01) inhibited AICD in a concentration-dependent manner (EC50=6.3 × 10−8 M; maximum inhibition 54.8±6.3% at 1 × 10−6 M). The L-glutamate inhibitory effect was pharmacologically characterized as mediated by group I mGlu receptors, since mGlu receptor agonists reproduced this effect. The EC50 values were: 3.2 × 10−7 M for (1S,3R)-ACPD; 4.5 × 10−8 M for quisqualate; 1.0 × 10−6 M for (S)-3,5-DHPG; 2.0 × 10−5 M for CHPG. Group I mGlu receptor antagonists inhibited the effects of quisqualate 1.0 × 10−6 M. The IC50 values calculated were: 8.7 × 10−5, 4.3 × 10−6 and 6.3 × 10−7 M for AIDA, LY 367385 and MPEP, respectively. L-Glutamate (1 × 10−6 M; 18 h) significantly (P⩽0.05) inhibited FasL expression (40.8±11.3%) (cytofluorimetric analysis), whereas it did not affect Fas signalling. Expression of both mGlu1 and mGlu5 receptor mRNA by T lymphocytes and T-cell lines, as demonstrated by reverse transcriptase–PCR analysis, suggests that L-glutamate-mediated inhibition of AICD was exerted on T cells. These data depict a novel role for L-glutamate in the regulation of the immune response through group I mGlu receptor-mediated mechanisms. PMID:16751798

Identification morphométrique des populations de Tribolium ...

African Journals Online (AJOL)

SARAH

30 nov. 2017 ... Méthodologie et résultats : Trois populations ont été définies en fonction de la céréale (maïs, mil ou riz) sur laquelle T. castaneum a déroulé son cycle de développement. Chacune des populations est constituée de 30 adultes mâles. Sur chaque adulte, 19 variables ont été mesurées. Les distances entre ...

Platelet glycoprotein IaC807T polymorphisms and ischemic stroke in young Chinese Han population.

Science.gov (United States)

Zhang, J; Huang, D; Yang, J; An, H; Ojha, R; DU, C; Liu, R

2012-11-01

The objective of this study was to investigate the association between platelet glycoprotein (GP) Ia C807T polymorphisms and ischemic stroke in young Chinese Han Population. We conducted a case-control study in 92 consecutive young (ischemic stroke inpatients and outpatients, 86 elder ischemic stroke control (> 50 years), and 160 age- and sex-matched healthy control. Genotyping of platelet GP Ia C807Tpolymorphisms was performed by polymerase chain reaction followed by sequencing nucleic acid with dideoxy chain-termination method and an ABI PRISM3100 (Perkin-Elmer Co) genetic analyzer. Student's t-test, chi-square test, and logistic regression modeling were used for data significance analyses. Hypertension and smoking were found to be the independent risk factors for ischemic stroke patients (aged ischemic stroke patients (aged > 50 years). There was no significant difference observed in the T allele frequency of GPIa C807T polymorphisms between young stroke patients and corresponding controls. These findings suggest that there is no role of GPIa C807T polymorphisms in the development of young first-ever ischemic stroke in Chinese Han Population.

Task-specificity of unilateral anodal and dual-M1 tDCS effects on motor learning.

Science.gov (United States)

Karok, Sophia; Fletcher, David; Witney, Alice G

2017-01-08

Task-specific effects of transcranial direct current stimulation (tDCS) on motor learning were investigated in 30 healthy participants. In a sham-controlled, mixed design, participants trained on 3 different motor tasks (Purdue Pegboard Test, Visuomotor Grip Force Tracking Task and Visuomotor Wrist Rotation Speed Control Task) over 3 consecutive days while receiving either unilateral anodal over the right primary motor cortex (M1), dual-M1 or sham stimulation. Retention sessions were administered 7 and 28 days after the end of training. In the Purdue Pegboard Test, both anodal and dual-M1 stimulation reduced average completion time approximately equally, an improvement driven by online learning effects and maintained for about 1 week. The Visuomotor Grip Force Tracking Task and the Visuomotor Wrist Rotation Speed Control Task were associated with an advantage of dual-M1 tDCS in consolidation processes both between training sessions and when testing at long-term retention; both were maintained for at least 1 month. This study demonstrates that M1-tDCS enhances and sustains motor learning with different electrode montages. Stimulation-induced effects emerged at different learning phases across the tasks, which strongly suggests that the influence of tDCS on motor learning is dynamic with respect to the functional recruitment of the distributed motor system at the time of stimulation. Divergent findings regarding M1-tDCS effects on motor learning may partially be ascribed to task-specific consequences and the effects of offline consolidation. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

Directory of Open Access Journals (Sweden)

Cecilia S Lindestam Arlehamn

2016-07-01

Full Text Available We performed a quantitative analysis of the HLA restriction, antigen and epitope specificity of human pathogen specific responses in healthy individuals infected with M. tuberculosis (Mtb, in a South African cohort as a test case. The results estimate the breadth of T cell responses for the first time in the context of an infection and human population setting. We determined the epitope repertoire of eleven representative Mtb antigens and a large panel of previously defined Mtb epitopes. We estimated that our analytic methods detected 50-75% of the total response in a cohort of 63 individuals. As expected, responses were highly heterogeneous, with responses to a total of 125 epitopes detected. The 66 top epitopes provided 80% coverage of the responses identified in our study. Using a panel of 48 HLA class II-transfected antigen-presenting cells, we determined HLA class II restrictions for 278 epitope/donor recognition events (36% of the total. The majority of epitopes were restricted by multiple HLA alleles, and 380 different epitope/HLA combinations comprised less than 30% of the estimated Mtb-specific response. Our results underline the complexity of human T cell responses at a population level. Efforts to capture and characterize this broad and highly HLA promiscuous Mtb-specific T cell epitope repertoire will require significant peptide multiplexing efforts. We show that a comprehensive "megapool" of Mtb peptides captured a large fraction of the Mtb-specific T cells and can be used to characterize this response.

Patterns of failure and overall survival in patients with completely resected T3 N0 M0 non-small cell lung cancer

International Nuclear Information System (INIS)

Gould, Perry M.; Bonner, James A.; Sawyer, Timothy E.; Deschamps, Claude; Lange, Carla M.; Li Hongzhe

1999-01-01

Background: Previous studies of patients with surgically resected non-small cell lung cancer and chest wall invasion have shown conflicting results with respect to prognosis. Whether high-risk subsets of the T3 N0 M0 population exist with respect to patterns of failure and overall survival has been difficult to ascertain, owing to small numbers of patients in most series. Methods and Materials: A retrospective review was performed to determine patterns of failure and overall survival for patients with completely resected T3 N0 M0 non-small cell lung cancer. From 1979 to 1993, 92 evaluable patients underwent complete resection for T3 N0 M0 non-small cell lung cancer. The following potential prognostic factors were recorded from the history: tumor size, location, grade, histology, patient age, use of adjuvant radiation therapy (18 of 92 patients), and type of surgical procedure (chest wall or extrapleural resection). Results: The actuarial 2- and 4-year overall survival rates for the entire cohort were 48% and 35%, respectively. The actuarial local control at 4 years was 94%. Neither the type of surgical procedure performed nor the addition of thoracic radiation therapy impacted local control or overall survival. Conclusion: Patients with completely resected T3 N0 M0 non-small cell lung cancer have similar local control and overall survival irrespective of primary location, type of surgery performed, or use of adjuvant radiation therapy. Additionally, the tumor recurrence rate and overall survival found in this study support the placement of this group of patients in Stage IIB of the 1997 AJCC lung staging classification

Prevalence of Catalase (-21 A/T Gene Variant in South Indian (Tamil Population

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A. Lourdhu Mary

2014-01-01

Full Text Available Catalase, an endogenous antioxidant enzyme, is responsible for regulating reactive species levels. Several epidemiologic studies have suggested that single nucleotide polymorphism in catalase gene may be associated with many diseases. The genotype of CAT (-21 A/T point mutation in promoter region of catalase gene was determined by polymerase chain based restriction fragment length polymorphism analysis in the DNA of 100 healthy volunteers. The frequency of CAT (-21 A/T gene polymorphism AA, AT, and TT genotypes was found to be 7, 23, and 70 percent, respectively. The mutant “T” allele frequency was found to be 0.82 among the south Indian (Tamil population. Chi square analysis showed that the study population lies within the Hardy-Weinberg equilibrium. The wild type genotype (AA was found to be very low (7% and the mutant genotype (AT/TT was found to be more prevalent (93% among the south Indian population. This suggests that the high prevalence of mutant genotype may increase the susceptibility to oxidative stress associated diseases.

Deployment Area Selection and Land Withdrawal/Acquisition. Chapter 3. M-X/MPS (M-X/Multiple Protective Shelter). Volume 1. Affected Environment.

Science.gov (United States)

1981-10-02

unique vegetation include: range extensions, relict populations, unusual ecotypes, hybridization zones, aquatic or wetland vegetation, bald mountains...Cinna rood reed grass E Cirsium thistle E Citrullus vulgaris E Claytonia miner’s lettuce M E Clematis virgin’s bower M Cleome integrifolia M I T5291

Ideias sobre progresso técnico em Vilém Flusser e Gilbert Simondon

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Angélica Beatriz Castro Guimarães

2013-07-01

Full Text Available http://dx.doi.org/10.5007/1807-9288.2013v9n1p172   Esse artigo apresenta algumas ideias sobre o progresso técnico e a relação humano-máquina em Vilém Flusser e Gilbert Simondon. Os dois autores apresentam conceitos bem diferentes sobre a técnica, mas ambos projetam seus argumentos para a situação de intensificação técnica que ocorre a partir de meados do século XX. Simondon pensa a geração de objetos técnicos como modulação de intensidades. Flusser concebe o progresso técnico como uma escalada de abstração que não se encerra como modelo linear e acumulativo. Os dois autores pensam a situação de desorientação diante da aparente intencionalidade dos objetos técnicos. A partir dos conceitos propostos é possível pensar sobre a modulação do fluxo técnico pela arte no campo da sensibilidade.

The combined effect of the T2DM susceptibility genes is an important risk factor for T2DM in non-obese Japanese: a population based case-control study

Directory of Open Access Journals (Sweden)

Yamakawa-Kobayashi Kimiko

2012-02-01

Full Text Available Abstract Background Type 2 diabetes mellitus (T2DM is a complex endocrine and metabolic disorder. Recently, several genome-wide association studies (GWAS have identified many novel susceptibility loci for T2DM, and indicated that there are common genetic causes contributing to the susceptibility to T2DM in multiple populations worldwide. In addition, clinical and epidemiological studies have indicated that obesity is a major risk factor for T2DM. However, the prevalence of obesity varies among the various ethnic groups. We aimed to determine the combined effects of these susceptibility loci and obesity/overweight for development of T2DM in the Japanese. Methods Single nucleotide polymorphisms (SNPs in or near 17 susceptibility loci for T2DM, identified through GWAS in Caucasian and Asian populations, were genotyped in 333 cases with T2DM and 417 control subjects. Results We confirmed that the cumulative number of risk alleles based on 17 susceptibility loci for T2DM was an important risk factor in the development of T2DM in Japanese population (P P P = 0.88 for trend. Conclusions Our findings indicate that there is an etiological heterogeneity of T2DM between obese/overweight and non-obese subjects.

A gut-homing, oligoclonal CD4+ T cell population in severe-combined immunodeficient mice expressing a rearranged, transgenic class I-restricted alpha beta T cell receptor

DEFF Research Database (Denmark)

Reimann, J; Rudolphi, A; Spiess, S

1995-01-01

We studied the peripheral T cell compartment of H-2b severe combined immunodeficient (scid) mice that express a transgenic (tg) alpha beta T cell receptor (TcR) specific for the H-Y (male) epitope presented by the H-2 class I Db molecule. Large populations of CD3+ NK1.1-TCR beta T+ T cells were...

MERRF/MELAS overlap syndrome due to the m.3291T>C mutation.

Science.gov (United States)

Liu, Kaiming; Zhao, Hui; Ji, Kunqian; Yan, Chuanzhu

2014-03-01

We report the case of a 19-year-old Chinese female harboring the m.3291T>C mutation in the MT-TL1 gene encoding the mitochondrial transfer RNA for leucine. She presented with a complex phenotype characterized by progressive cerebellar ataxia, frequent myoclonus seizures, recurrent stroke-like episodes, migraine-like headaches with nausea and vomiting, and elevated resting lactate blood level. It is known that the myoclonus epilepsy with ragged-red fibers (MERRF) is characterized by cerebellar ataxia and myoclonus epilepsy, while that the mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is characterized by recurrent stroke-like episodes, migraine-like headaches, and elevated resting lactate blood level. So the patient's clinical manifestations suggest the presence of a MERRF/MELAS overlap syndrome. Muscle biopsy of the patient showed the presence of numerous scattered ragged-red fibers, some cytochrome c oxidase-deficient fibers, and several strongly succinate dehygrogenase-reactive vessels, suggestive of a mitochondrial disorder. Direct sequencing of the complete mitochondrial genome of the proband revealed no mutations other than the T-to-C transition at nucleotide position 3291. Restriction fragment length polymorphism analysis of the proband and her family revealed maternal inheritance of the mutation in a heteroplasmic manner. The analysis of aerobic respiration and glycolysis demonstrated that the fibroblasts from the patient had mitochondrial dysfunction. Our results suggest that the m.3291T>C is pathogenic. This study is the first to describe the m.3291T>C mutation in association with the MERRF/MELAS overlap syndrome.

Understanding delayed T-cell priming, lung recruitment, and airway luminal T-cell responses in host defense against pulmonary tuberculosis.

Science.gov (United States)

Shaler, Christopher R; Horvath, Carly; Lai, Rocky; Xing, Zhou

2012-01-01

Mycobacterium tuberculosis (M.tb), the causative bacterium of pulmonary tuberculosis (TB), is a serious global health concern. Central to M.tb effective immune avoidance is its ability to modulate the early innate inflammatory response and prevent the establishment of adaptive T-cell immunity for nearly three weeks. When compared with other intracellular bacterial lung pathogens, such as Legionella pneumophila, or even closely related mycobacterial species such as M. smegmatis, this delay is astonishing. Customarily, the alveolar macrophage (AM) acts as a sentinel, detecting and alerting surrounding cells to the presence of an invader. However, in the case of M.tb, this may be impaired, thus delaying the recruitment of antigen-presenting cells (APCs) to the lung. Upon uptake by APC populations, M.tb is able to subvert and delay the processing of antigen, MHC class II loading, and the priming of effector T cell populations. This delay ultimately results in the deferred recruitment of effector T cells to not only the lung interstitium but also the airway lumen. Therefore, it is of upmost importance to dissect the mechanisms that contribute to the delayed onset of immune responses following M.tb infection. Such knowledge will help design the most effective vaccination strategies against pulmonary TB.

Highlights of X-Stack ExM Deliverable Swift/T

Energy Technology Data Exchange (ETDEWEB)

Wozniak, Justin M. [Argonne National Lab. (ANL), Argonne, IL (United States)

2016-03-31

Swift/T is a key success from the ExM: System support for extreme-scale, many-task applications1 X-Stack project, which proposed to use concurrent dataflow as an innovative programming model to exploit extreme parallelism in exascale computers. The Swift/T component of the project reimplemented the Swift language from scratch to allow applications that compose scientific modules together to be build and run on available petascale computers (Blue Gene, Cray). Swift/T does this via a new compiler and runtime that generates and executes the application as an MPI program. We assume that mission-critical emerging exascale applications will be composed as scalable applications using existing software components, connected by data dependencies. Developers wrap native code fragments using a higherlevel language, then build composite applications to form a computational experiment. This exemplifies hierarchical concurrency: lower-level messaging libraries are used for fine-grained parallelism; highlevel control is used for inter-task coordination. These patterns are best expressed with dataflow, but static DAGs (i.e., other workflow languages) limit the applications that can be built; they do not provide the expressiveness of Swift, such as conditional execution, iteration, and recursive functions.

Human skin is protected by four functionally and phenotypically discrete populations of resident and recirculating memory T cells

Science.gov (United States)

Watanabe, Rei; Gehad, Ahmed; Yang, Chao; Campbell, Laura; Teague, Jessica E.; Schlapbach, Christoph; Elco, Christopher; Huang, Victor; Matos, Tiago R.; Kupper, Thomas S.; Clark, Rachael A.

2015-01-01

The skin of an adult human contains approximately 20 billion memory T cells. Epithelial barrier tissues are infiltrated by a combination of resident and recirculating T cells in mice but the relative proportions and functional activities of resident versus recirculating T cells have not been evaluated in human skin. We discriminated resident from recirculating T cells in human engrafted mice and lymphoma patients using alemtuzumab, a medication that depletes recirculating T cells from skin, and then analyzed these T cell populations in healthy human skin. All non-recirculating resident memory T cells (TRM) expressed CD69, but the majority were CD4+, CD103− and located in the dermis, in contrast to studies in mice. Both CD4+ and CD8+ CD103+ TRM were enriched in the epidermis, had potent effector functions and had a limited proliferative capacity compared to CD103− TRM. TRM of both types had more potent effector functions than recirculating T cells. Induction of CD103 on human T cells was enhanced by keratinocyte contact, depended on TGFβ and was independent of T cell keratinocyte adhesive interactions. We observed two distinct populations of recirculating T cells, CCR7+/L-selectin+ central memory T cells (TCM) and CCR7+/L-selectin− T cells, which we term migratory memory T cells (TMM). Circulating skin-tropic TMM were intermediate in cytokine production between TCM and effector memory T cells. In patients with cutaneous T cell lymphoma, malignant TCM and TMM induced distinct inflammatory skin lesions and TMM were depleted more slowly from skin after alemtuzumab, suggesting TMM may recirculate more slowly. In summary, human skin is protected by four functionally distinct populations of T cells, two resident and two recirculating, with differing territories of migration and distinct functional activities. PMID:25787765

Radiotherapy or surgery for T2N0M0 glottic carcinoma?

International Nuclear Information System (INIS)

Stalpers, L.J.A.; Daal, W.A.J. van; Verbeek, A.L.M.

1989-01-01

Decision analysis was used to evaluate the results of treatment of T 2 N 0 M 0 glottic carcinoma as presented in the literature. Based on mean values for recurrence, salvage eligibility after recurrence and salvage success, the 5-year survival after radiotherapy and surgery proved to be almost identical, 85 and 86%. If the recurrence rates and the salvage rates were varied, a marginal advantage for surgery in small tumours and a major advantage in more extended tumours was seen if only survival is considered. To take the quality of speech into account, a utility analysis was performed. A utility scale was defined ranging from 0.0 as the value for death, to 1.0 for a successfully irradiated patient with preservation of normal speech. A utility of 0.99 or less for the laryngectomized patient would favour radiotherapy over surgery for all T 2 tumours. In patients with T 2b tumours and in extreme circumstances, e.g. if failure rates of radiotherapy are extremely low, an exact assessment of patient utilities may be pivotal. Under normal circumstances radiotherapy is preferred for T 2 N 0 M 0 glottic carcinoma if both survival and the quality of speech are taken into account. (author). 39 refs.; 5 figs.; 1 tab

Spectrum of MTHFR gene SNPs C677T and A1298C: a study among 23 population groups of India.

Science.gov (United States)

Saraswathy, Kallur Nava; Asghar, Mohammad; Samtani, Ratika; Murry, Benrithung; Mondal, Prakash Ranjan; Ghosh, Pradeep Kumar; Sachdeva, Mohinder Pal

2012-04-01

Elevated homocysteine is a risk factor for many complex disorders. The role of methylenetetrahydrofolate reductase (MTHFR) gene in methylation of homocysteine makes it one of the most important candidate genes for these disorders. Considering the heterogeneity in its distribution in world populations, we screened MTHFR C677T and A1298C single nucleotide polymorphisms in a total of 23 Indian caste, tribal and religious population groups from five geographical regions of India and belonging to four major linguistic groups. The frequencies of MTHFR 677T and 1298C alleles were found to be 10.08 and 20.66%, respectively. MTHFR homozygous genotype 677TT was absent in eight population groups and homozygous 1298CC was absent in two population groups. 677T allele was found to be highest among north Indian populations with Indo-European tongue and 1298C was high among Dravidian-speaking tribes of east India and south India. The less common mutant haplotype 677T-1298C was observed among seven population groups and overall the frequency of this haplotype was 0.008, which is similar to that of African populations. cis configuration of 677T and 1298C was 0.94%. However, we could not find any individual with four mutant alleles which supports the earlier observation that presence of more than two mutant alleles may decrease the viability of foetus and possibly be a selective disadvantage in the population.

Robert Frazure mälestuspuu Sõpruse pargis sai graniitpotil tähistuse / Raivo Feldmann

Index Scriptorium Estoniae

Feldmann, Raivo

2007-01-01

Esimese USA suursaadiku sõjajärgses Eestis, Robert Frazure' mälestuspuu sai Sõpruse pargis tähise, vajaliku raha ettevõtmise heaks annetasid Ameerika Suursaatkonna töötajad, nende delegatsioon viibis Põltsamaal Sõpruse pargis toimunud üritusel

The class characteristic mark of the H&M Mul-T-Lock picking tool in toolmarks examination.

Science.gov (United States)

Volkov, Nikolai; Finkelstein, Nir; Novoselsky, Yehuda; Tsach, Tsadok

2014-07-01

Mul-T-Lock is a high security lock cylinder distinguished by the use of a telescoping "pin-in-pin"-tumbler design. Picking the Mul-T-Lock cylinder with a traditional picking tool is highly complicated because it can get stuck between the inner and outer pins. The H&M Mul-T-Lock picking tool was designed to overcome this problem and facilitate the picking of the "pin-in-pin" cylinder. The purpose of this research is to determine whether H&M Mul-T-Lock picking tool leaves class characteristic mark and whether it can be distinguished from traditional picking tools marks and from regular key marks. It also describes and determines the class characteristic mark left on telescopic pins, its origin, recurrence, and its benefit to the toolmarks examiner. When receiving a Mul-T-Lock from a crime scene, a toolmarks examiner can quickly determine whether or not it was picked by an H&M Mul-T-Lock picking tool by noticing the class characteristic mark which this typical tool leaves. © 2014 American Academy of Forensic Sciences.

Kolm tähtsat raamatut Eesti lähiajaloo mõistmiseks / Mai Beijer

Index Scriptorium Estoniae

Beijer, Mai

2007-01-01

rets. rmt.: Laar, Mart. September 1944 : Otto Tiefi valitsus. Tallinn : Varrak, 2007. Paju, Imbi. Förträngda minnen/ soome keelest tõlkinud Heidi Granqvist. Stockholm : Atlantis, 2007; eesti keeles: Tõrjutud mälestud, 2007. Kross, Jaan. På stället flyg/eesti keelest tõlkinud Enel Mellberg. Stockholm : Natur & Kultur, 2007; originaali pealkiri: Paigallend, 1998

Teadlased: Juštšenkole anti väga puhast mürki

Index Scriptorium Estoniae

2009-01-01

Suurbritannia ajakiri Lancet väidab, et dioksiin, mis põhjustas Ukraina presidendile Viktor Juštšenkole 2004. aastal tõsise mürgituse, oli nii puhas, et pidi pärinema otse laboratooriumist, kuna loodusest ei saa puhast dioksiini

Surface Ig on rabbit lymphocytes. Rabbit B and T cells are distinct populations

NARCIS (Netherlands)

Bast, B J; Catty, D; Manten-Slingerland, R; Jansen, J T; Veldhuis, Dick H.; Roholl, P; Ballieux, R E

1979-01-01

Rabbit peripheral blood lymphocytes (PBL) were analyzed by immunofluorescence using anti-T cell conjugates and anti-Fab, anti-a1 allotype, anti-IgM and anti-IgA conjugates. In addition, T cells were demonstrated by rosetting with papain-treated homologous erythrocytes. Control experiments, using

A STUDY OF THE DIVERSE T DWARF POPULATION REVEALED BY WISE

International Nuclear Information System (INIS)

Mace, Gregory N.; Wright, Edward L.; McLean, Ian S.; Davy Kirkpatrick, J.; Gelino, Christopher R.; Griffith, Roger L.; Mix, Katholeen; Beichman, Charles A.; Lowrance, Patrick J.; Cushing, Michael C.; Skrutskie, Michael F.; Marsh, Kenneth A.; Eisenhardt, Peter R.; Thompson, Maggie A.; Bailey, Vanessa; Hinz, Philip M.; Knox, Russell P.; Bloom, Joshua S.; Burgasser, Adam J.; Fortney, Jonathan J.

2013-01-01

We report the discovery of 87 new T dwarfs uncovered with the Wide-field Infrared Survey Explorer (WISE) and 3 brown dwarfs with extremely red near-infrared colors that exhibit characteristics of both L and T dwarfs. Two of the new T dwarfs are likely binaries with L7 ± 1 primaries and mid-type T secondaries. In addition, our follow-up program has confirmed 10 previously identified T dwarfs and 4 photometrically selected L and T dwarf candidates in the literature. This sample, along with the previous WISE discoveries, triples the number of known brown dwarfs with spectral types later than T5. Using the WISE All-Sky Source Catalog we present updated color-color and color-type diagrams for all the WISE-discovered T and Y dwarfs. Near-infrared spectra of the new discoveries are presented along with spectral classifications. To accommodate later T dwarfs we have modified the integrated flux method of determining spectral indices to instead use the median flux. Furthermore, a newly defined J-narrow index differentiates the early-type Y dwarfs from late-type T dwarfs based on the J-band continuum slope. The K/J indices for this expanded sample show that 32% of late-type T dwarfs have suppressed K-band flux and are blue relative to the spectral standards, while only 11% are redder than the standards. Comparison of the Y/J and K/J index to models suggests diverse atmospheric conditions and supports the possible re-emergence of clouds after the L/T transition. We also discuss peculiar brown dwarfs and candidates that were found not to be substellar, including two young stellar objects and two active galactic nuclei. The substantial increase in the number of known late-type T dwarfs provides a population that will be used to test models of cold atmospheres and star formation. The coolest WISE-discovered brown dwarfs are the closest of their type and will remain the only sample of their kind for many years to come.

Baseline natural killer and T cell populations correlation with virologic outcome after regimen simplification to atazanavir/ritonavir alone (ACTG 5201.

Directory of Open Access Journals (Sweden)

John E McKinnon

Full Text Available Simplified maintenance therapy with ritonavir-boosted atazanavir (ATV/r provides an alternative treatment option for HIV-1 infection that spares nucleoside analogs (NRTI for future use and decreased toxicity. We hypothesized that the level of immune activation (IA and recovery of lymphocyte populations could influence virologic outcomes after regimen simplification.Thirty-four participants with virologic suppression ≥ 48 weeks on antiretroviral therapy (2 NRTI plus protease inhibitor were switched to ATV/r alone in the context of the ACTG 5201 clinical trial. Flow cytometric analyses were performed on PBMC isolated from 25 patients with available samples, of which 24 had lymphocyte recovery sufficient for this study. Assessments included enumeration of T-cells (CD4/CD8, natural killer (NK (CD3+CD56+CD16+ cells and cell-associated markers (HLA-DR, CD's 38/69/94/95/158/279.Eight of the 24 patients had at least one plasma HIV-1 RNA level (VL >50 copies/mL during the study. NK cell levels below the group median of 7.1% at study entry were associated with development of VL >50 copies/mL following simplification by regression and survival analyses (p = 0.043 and 0.023, with an odds ratio of 10.3 (95% CI: 1.92-55.3. Simplification was associated with transient increases in naïve and CD25+ CD4+ T-cells, and had no impact on IA levels.Lower NK cell levels prior to regimen simplification were predictive of virologic rebound after discontinuation of nucleoside analogs. Regimen simplification did not have a sustained impact on markers of IA or T lymphocyte populations in 48 weeks of clinical monitoring.ClinicalTrials.gov NCT00084019.

Principales espèces médicinales utilisées en médecine vétérinaire ...

African Journals Online (AJOL)

Principales espèces médicinales utilisées en médecine vétérinaire au Bénin: disponibilité et caractéristiques dendrométriques. Irvine Yèïnou Minaflinou Sacca Sidi, Pascal Abiodoun Olounladé, Alain Yaoitcha, Vidjinnangni Fifamè Grâce Nadège Dedehou, Géorcelin Goué Alowanou, Erick Virgile Bertrand Azando, Mawulé ...

Sobrecarga térmica em fábrica de móveis

Directory of Open Access Journals (Sweden)

José Reinaldo Moreira da Silva

Full Text Available Atualmente, há uma busca incessante pela qualidade dos ambientes industriais. Estudos demonstram que o desenvolvimento e o envolvimento dos trabalhadores em programas de qualidade nas indústrias trazem muitos benefícios administrativos, produtivos e pessoais. Nesses programas de qualidade, encontram-se as condições de conforto nos ambientes de trabalho, principalmente com relação à temperatura. No Brasil, é obrigatório que ambientes de trabalho tenham condições adequadas, sem que ocorram riscos de sobrecarga térmica aos trabalhadores. A avaliação quantitativa da sobrecarga térmica é feita pelo Índice de Bulbo Úmido Termômetro de Globo (IBUTG. O objetivo deste trabalho foi avaliar o IBUTG e as condições térmicas no setor de corte de MDF, situado em uma fábrica de móveis. A avaliação da exposição ocupacional ao calor foi realizada utilizando-se aparelho medidor de stress térmico digital, além de técnicas da segurança do trabalho e de higiene ocupacional. A avaliação consistiu em utilizar temperaturas provenientes de termômetros de bulbo úmido, bulbo seco e de globo. De posse dessas temperaturas e do tipo de atividade desenvolvida pelo trabalhador, no posto de trabalho, foram analisadas as condições térmicas locais. Os resultados demonstraram que, com relação à temperatura e à atividade, os funcionários não estão expostos à sobrecarga térmica, ou seja, o ambiente não é insalubre para o agente físico calor. Já com relação ao conforto térmico, esse ambiente de trabalho não foi considerado adequado. As temperaturas efetivas avaliadas ficaram acima dos limites definidos pela ergonomia ambiental.

LHS 2803B: A VERY WIDE MID-T DWARF COMPANION TO AN OLD M DWARF IDENTIFIED FROM PAN-STARRS1

International Nuclear Information System (INIS)

Deacon, Niall R.; Liu, Michael C.; Magnier, Eugene A.; Bowler, Brendan P.; Mann, Andrew W.; Burgett, William S.; Chambers, Ken C.; Kaiser, Nick; Kudritzki, Rolf-Peter; Morgan, Jeff S.; Tonry, John L.; Wainscoat, Richard J.; Redstone, Joshua A.; Hodapp, Klaus W.; Price, Paul A.

2012-01-01

We report the discovery of a wide (∼1400 AU projected separation), common proper motion companion to the nearby M dwarf LHS 2803 (PSO J207.0300-13.7422). This object was discovered during our census of the local T dwarf population using Pan-STARRS1 and Two Micron All Sky Survey data. Using the Infrared Telescope Facility/SpeX near-infrared spectroscopy, we classify the secondary to be spectral type T5.5. University of Hawaii 2.2 m/SuperNova Integral Field Spectrograph optical spectroscopy indicates that the primary has a spectral type of M4.5, with approximately solar metallicity and no measurable Hα emission. We use this lack of activity to set a lower age limit for the system of 3.5 Gyr. Using a comparison with chance alignments of brown dwarfs and nearby stars, we conclude that the two objects are unlikely to be a chance association. The primary's photometric distance of 21 pc and its proper motion implies thin disk kinematics. Based on these kinematics and its metallicity, we set an upper age limit for the system of 10 Gyr. Evolutionary model calculations suggest that the secondary has a mass of 72± 4 7 M Jup , temperature of 1120 ± 80 K, and log g = 5.4 ± 0.1 dex. Model atmosphere fitting to the near-IR spectrum gives similar physical parameters of 1100 K and log g = 5.0.

LHS 2803B: A VERY WIDE MID-T DWARF COMPANION TO AN OLD M DWARF IDENTIFIED FROM PAN-STARRS1

Energy Technology Data Exchange (ETDEWEB)

Deacon, Niall R. [Max Planck Institute for Astronomy, Koenigstuhl 17, D-69117 Heidelberg (Germany); Liu, Michael C.; Magnier, Eugene A.; Bowler, Brendan P.; Mann, Andrew W.; Burgett, William S.; Chambers, Ken C.; Kaiser, Nick; Kudritzki, Rolf-Peter; Morgan, Jeff S.; Tonry, John L.; Wainscoat, Richard J. [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); Redstone, Joshua A. [Facebook, 1601 Willow Road, Menlo Park, CA 94025 (United States); Hodapp, Klaus W. [Institute for Astronomy, University of Hawaii, 640 North Aohoku Place, Hilo, HI 96720 (United States); Price, Paul A., E-mail: deacon@mpia.de [Princeton University Observatory, 4 Ivy Lane, Peyton Hall, Princeton University, Princeton, NJ 08544 (United States)

2012-09-20

We report the discovery of a wide ({approx}1400 AU projected separation), common proper motion companion to the nearby M dwarf LHS 2803 (PSO J207.0300-13.7422). This object was discovered during our census of the local T dwarf population using Pan-STARRS1 and Two Micron All Sky Survey data. Using the Infrared Telescope Facility/SpeX near-infrared spectroscopy, we classify the secondary to be spectral type T5.5. University of Hawaii 2.2 m/SuperNova Integral Field Spectrograph optical spectroscopy indicates that the primary has a spectral type of M4.5, with approximately solar metallicity and no measurable H{alpha} emission. We use this lack of activity to set a lower age limit for the system of 3.5 Gyr. Using a comparison with chance alignments of brown dwarfs and nearby stars, we conclude that the two objects are unlikely to be a chance association. The primary's photometric distance of 21 pc and its proper motion implies thin disk kinematics. Based on these kinematics and its metallicity, we set an upper age limit for the system of 10 Gyr. Evolutionary model calculations suggest that the secondary has a mass of 72{+-}{sup 4}{sub 7} M{sub Jup}, temperature of 1120 {+-} 80 K, and log g = 5.4 {+-} 0.1 dex. Model atmosphere fitting to the near-IR spectrum gives similar physical parameters of 1100 K and log g = 5.0.

Primary resistance to osimertinib due to SCLC transformation: Issue of T790M determination on liquid re-biopsy.

Science.gov (United States)

Minari, R; Bordi, P; Del Re, M; Facchinetti, F; Mazzoni, F; Barbieri, F; Camerini, A; Comin, C E; Gnetti, L; Azzoni, C; Nizzoli, R; Bortesi, B; Rofi, E; Petreni, P; Campanini, N; Rossi, G; Danesi, R; Tiseo, M

2018-01-01

EGFR T790M mutation is the most common mechanism of resistance to first-/second-generation EGFR tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC) and could be overcome by third-generation EGFR-TKIs, such as osimertinib. Liquid biopsy, a non-invasive technique used to test the presence of the resistant mutation, may help avoiding tissue re-biopsy. However, analysing only circulating-free DNA, information about other less frequent and coexisting resistance mechanisms may remain unrevealed. All patients reported in this series participated in the ASTRIS trial, a real world treatment study testing the efficacy of osimertinib (80mg os die) in advanced T790M-positive NSCLC progressed to prior EGFR-TKI. Patients were considered eligible to osimertinib if T790M positive on tissue or plasma samples. In our patients, EGFR molecular testing on blood sample was conducted with digital droplet PCR (ddPCR). We report our experience of five patients treated with osimertinib after T790M detection on liquid biopsy that presented a disease progression at first tumor assessment mediated by SCLC transformation, as evidenced at tissue re-biopsies. All patients showed low ratio T790M/activating mutation in the blood before osimertinib (lower than 0.03). For three patients, EGFR mutational analysis was T790M-negative when re-assessed by using a less sensitive method (therascreen ® ) on the same liquid biopsy sample analysed by ddPCR before osimertinib therapy. Although liquid biopsy is a relevant tool to diagnose T790M presence in NSCLC patients resistant to EGFR-TKI, in case of a low ratio T790M/activating mutation, tissue biopsy should be considered to exclude the presence of SCLC transformation and/or other concomitant resistance mechanisms. Copyright © 2017 Elsevier B.V. All rights reserved.

Test of lithium capillary-pore systems on the T-11M tokamak

International Nuclear Information System (INIS)

Evtikhin, V.A.

2002-01-01

In this work the divertor plate behavior has been simulated in the quasi-stationary condition. In the previous experiments on T-11M the CPS quasi-stationary heat state has not been achieved for pulse length (≤0.1 s). The T-11M tokamak up-grade allowed its performance to be increased as follows: plasma current up to 100 kA, pulse length 0.2-0.3 s. The new lithium limiter unlike the previous versions has a thermal regulation system which permits a lithium surface initial temperature to be given from -196 to 600 deg. C. This provides for an increase in test parameter range: sorption and desorption of plasma-forming gas, lithium emission into discharge, lithium erosion, limiter deposited power and so on. The first results of experiments were presented. (author)

Responses of mRNA expression of PepT1 in small intestine to ...

African Journals Online (AJOL)

To study the effect of circulation small peptides concentration on mRNA expression in small intestine, graded amount of soybean small peptides (SSP) were infused into lactating goats through duodenal fistulas. Peptide-bound amino acid (PBAA) concentration in arterial plasma and the mRNA expression of PepT1 was ...

Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells.

Science.gov (United States)

Shimizu, Kanako; Shinga, Jun; Yamasaki, Satoru; Kawamura, Masami; Dörrie, Jan; Schaft, Niels; Sato, Yusuke; Iyoda, Tomonori; Fujii, Shin-Ichiro

2015-01-01

Cell-based therapies using genetically engineered lymphocytes expressing antigen-specific T cell receptors (TCRs) hold promise for the treatment of several types of cancers. Almost all studies using this modality have focused on transfer of TCR from CD8 cytotoxic T lymphocytes (CTLs). The transfer of TCR from innate lymphocytes to other lymphocytes has not been studied. In the current study, innate and adaptive lymphocytes were transfected with the human NKT cell-derived TCRα and β chain mRNA (the Vα24 and Vβ11 TCR chains). When primary T cells transfected with NKT cell-derived TCR were subsequently stimulated with the NKT ligand, α-galactosylceramide (α-GalCer), they secreted IFN-γ in a ligand-specific manner. Furthermore when γδT cells were transfected with NKT cell-derived TCR mRNA, they demonstrated enhanced proliferation, IFN-γ production and antitumor effects after α-GalCer stimulation as compared to parental γδT cells. Importantly, NKT cell TCR-transfected γδT cells responded to both NKT cell and γδT cell ligands, rendering them bi-potential innate lymphocytes. Because NKT cell receptors are unique and universal invariant receptors in humans, the TCR chains do not yield mispaired receptors with endogenous TCR α and β chains after the transfection. The transfection of NKT cell TCR has the potential to be a new approach to tumor immunotherapy in patients with various types of cancer.

Retention of Ag-specific memory CD4+ T cells in the draining lymph node indicates lymphoid tissue resident memory populations.

Science.gov (United States)

Marriott, Clare L; Dutton, Emma E; Tomura, Michio; Withers, David R

2017-05-01

Several different memory T-cell populations have now been described based upon surface receptor expression and migratory capabilities. Here we have assessed murine endogenous memory CD4 + T cells generated within a draining lymph node and their subsequent migration to other secondary lymphoid tissues. Having established a model response targeting a specific peripheral lymph node, we temporally labelled all the cells within draining lymph node using photoconversion. Tracking of photoconverted and non-photoconverted Ag-specific CD4 + T cells revealed the rapid establishment of a circulating memory population in all lymph nodes within days of immunisation. Strikingly, a resident memory CD4 + T cell population became established in the draining lymph node and persisted for several months in the absence of detectable migration to other lymphoid tissue. These cells most closely resembled effector memory T cells, usually associated with circulation through non-lymphoid tissue, but here, these cells were retained in the draining lymph node. These data indicate that lymphoid tissue resident memory CD4 + T-cell populations are generated in peripheral lymph nodes following immunisation. © 2017 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Açık kaynak kodlu kütüphane otomosyon sistemlerinin akademik kütüphanelerde kullanımı

OpenAIRE

SALİHOĞLU, Remzi

2012-01-01

       Kütüphanelerin yönetimi için bütünleşik kütüphane otomasyon sistemleri geliştirilmiştir. Bu sistemler kütüphane işlemlerinin birlikte yürütülmesini sağlayan elektronik yazılımlardan oluşmaktadır. Bir kütüphanede yürütülen hizmetlerin büyük oranda kütüphane otomasyon sistemi üzerinden gerçekleştirilmesi mümkündür.
Türkiye’de yerli kütüphane otomasyon sistemleri üretilmektedir. Bunların çoğu ticari olarak geliştirile...

Automatizace útoku MitM na WiFi sítích

OpenAIRE

Vondráček, Martin

2016-01-01

Tato bakalářská práce se zaměřuje na výzkum v oblasti bezpečnosti bezdrátových sítí. Práce přináší studii široce využívaných síťových technologií a principů zajištění bezpečnosti bezdrátových sítí. Analyzované technologie a způsoby zabezpečení trpí slabinami, které mohou být zneužity k provedení útoku MitM . Práce zahrnuje přehled dostupných nástrojů zaměřených na využití jednotlivých slabin. Výsledkem této práce je balíček wifimitm a CLI nástroj, oba implementované v jazyce Python. Balíček p...

Association of Methylenetetrahydrofolate Reductase C677T Polymorphism with Hyperhomocysteinemia and Deep Vein Thrombosis in the Iranian Population.

Science.gov (United States)

Ghaznavi, Habib; Soheili, Zahra; Samiei, Shahram; Soltanpour, Mohammad Soleiman

2015-12-01

Deep venous thrombosis (DVT) is a common but elusive condition characterized by a high morbidity and mortality rate. The aim of the present study was to investigate the correlation between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism with plasma total homocysteine (tHcy) levels and DVT risk in an Iranian population. Our study population consisted of 67 patients with a diagnosis of DVT and 67 healthy subjects as controls. Genotyping of MTHFR C677T polymorphism was performed by the polymerase chain reaction technique combined with restriction enzyme fragment length polymorphism (PCR-RFLP) and measurement of tHcy levels was done by enzyme immunoassay method. Plasma tHcy levels were significantly higher in DVT patients than controls (18.09±7.6 vs. 10.5±4.3, P=0.001). Also, plasma tHcy levels were significantly higher in MTHFR 677TT genotypes compared to 677CC genotypes in both DVT patients (P=0.016) and controls (P=0.03). Neither heterozygote nor homozygote genotypes of MTHFR C677T polymorphism was significantly correlated with DVT (P>0.05). The distribution of MTHFR C677T genotypes was similar between men and women in both DVT patients and controls (P>0.05). Moreover, the frequency of mutant 677T allele did not differ significantly between the two groups (28.3% vs. 21.6%, P=0.15). Based on this study, we propose that hyperhomocysteinemia but not homozygosity for MTHFR C677T polymorphism is a significant risk factor for DVT in the Iranian population. Also, MTHFR 677TT genotype is a determinant of elevated plasma tHcy levels.

Elektronický kompas s tříosým magnetometrem

OpenAIRE

Okoun, Petr

2013-01-01

Tato semestrální práce je zaměřena navržení elektronického kompasu s tříosým magnetometrem, který odstraňuje nedostatky magnetického kompasu a je použitelný na libovolném místě zemského povrchu. Kompas je schopen měřit azimut správně pouze tehdy, je-li zařízení ve vodorovné poloze. Vzhledem ke skutečnosti, že tuto podmínku nelze vždy splnit je součástí projektu i řešení kompenzace náklonu kompasu pomocí akcelerometru. This semestral thesis deals with development of electronic compass with ...

Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9.

Science.gov (United States)

Jackman, Jane E; Montange, Rebecca K; Malik, Harmit S; Phizicky, Eric M

2003-05-01

Methylation of tRNA at the N-1 position of guanosine to form m(1)G occurs widely in nature. It occurs at position 37 in tRNAs from all three kingdoms, and the methyltransferase that catalyzes this reaction is known from previous work of others to be critically important for cell growth in Escherichia coli and the yeast Saccharomyces cerevisiae. m(1)G is also widely found at position 9 in eukaryotic tRNAs, but the corresponding methyltransferase was unknown. We have used a biochemical genomics approach with a collection of purified yeast GST-ORF fusion proteins to show that m(1)G(9) formation of yeast tRNA(Gly) is associated with ORF YOL093w, named TRM10. Extracts lacking Trm10p have undetectable levels of m(1)G(9) methyltransferase activity but retain normal m(1)G(37) methyltransferase activity. Yeast Trm10p purified from E. coli quantitatively modifies the G(9) position of tRNA(Gly) in an S-adenosylmethionine-dependent fashion. Trm10p is responsible in vivo for most if not all m(1)G(9) modification of tRNAs, based on two results: tRNA(Gly) purified from a trm10-Delta/trm10-Delta strain is lacking detectable m(1)G; and a primer extension block occurring at m(1)G(9) is removed in trm10-Delta/trm10-Delta-derived tRNAs for all 9 m(1)G(9)-containing species that were testable by this method. There is no obvious growth defect of trm10-Delta/trm10-Delta strains. Trm10p bears no detectable resemblance to the yeast m(1)G(37) methyltransferase, Trm5p, or its orthologs. Trm10p homologs are found widely in eukaryotes and many archaea, with multiple homologs in several metazoans, including at least three in humans.

Phenotypic and functional profiling of CD4 T cell compartment in distinct populations of healthy adults with different antigenic exposure.

Directory of Open Access Journals (Sweden)

Sophie Roetynck

Full Text Available Multiparameter flow cytometry has revealed extensive phenotypic and functional heterogeneity of CD4 T cell responses in mice and humans, emphasizing the importance of assessing multiple aspects of the immune response in correlation with infection or vaccination outcome. The aim of this study was to establish and validate reliable and feasible flow cytometry assays, which will allow us to characterize CD4 T cell population in humans in field studies more fully.We developed polychromatic flow cytometry antibody panels for immunophenotyping the major CD4 T cell subsets as well as broadly characterizing the functional profiles of the CD4 T cells in peripheral blood. We then validated these assays by conducting a pilot study comparing CD4 T cell responses in distinct populations of healthy adults living in either rural or urban Kenya. This study revealed that the expression profile of CD4 T cell activation and memory markers differed significantly between African and European donors but was similar amongst African individuals from either rural or urban areas. Adults from rural Kenya had, however, higher frequencies and greater polyfunctionality among cytokine producing CD4 T cells compared to both urban populations, particularly for "Th1" type of response. Finally, endemic exposure to malaria in rural Kenya may have influenced the expansion of few discrete CD4 T cell populations with specific functional signatures.These findings suggest that environmentally driven T cell activation does not drive the dysfunction of CD4 T cells but is rather associated with greater magnitude and quality of CD4 T cell response, indicating that the level or type of microbial exposure and antigenic experience may influence and shape the functionality of CD4 T cell compartment. Our data confirm that it is possible and mandatory to assess multiple functional attributes of CD4 T cell response in the context of infection.

Femoral chondrosarcoma discovered by SPECT-T.D.M. osseous scintigraphy; Chondrosarcome femoral decouvert par scintigraphie osseuse SPECT-TDM

Energy Technology Data Exchange (ETDEWEB)

Bourahla, K.; Nguyen, L.; Rolling, P.; Schott, A.; Di Salvo, R. [CLCC Paul-Strauss, Strasbourg, (France)

2009-05-15

Objectives: the SPECT/T.D.M. imaging can allow a quick diagnosis direction by the concomitant exploitation of scintigraphy data and morphological data brought by the T.D.M.. We present the case of a chondrosarcoma diagnosed in nuclear medicine thanks to the exploitation of the data supplied by the SPECT/T.D.M. that allowed to direct rapidly the later explorations while reducing the time of the patient coverage. Conclusions: A knowledge of the most characteristic morphological data allows to give some diagnosis at first sight during the scintigraphy explorations coupled SPECT/T.D.M. and this in the interest of the patient by reducing his coverage while including the nuclear doctor more broadly in some diagnosis approaches previously remote from his reach. (N.C.)

Flares of Orion population variables in the association Taurus T3

International Nuclear Information System (INIS)

Khodzhaev, A.S.; AN Armyanskoj SSR, Byurakan. Astrofizicheskaya Observatoriya)

1987-01-01

Thirteen new flare stars, proved to be irregular variables of Orion Population, were discovered from a study of the Taurus Dark Cloud region by the homogeneous photographic multipose method on the wide angle Schmidt telescopes of the Byurakan Astorphysical Observatory. Seventeen flares on these stars were detected for about 750 hours of the effective observing time. The analysis of the complicated light curves of these flares shows a great variety and multiplicity of this phenomenon and various dynamics of flare energy release processes. The existence of flare stars with some properties typical for both of the T Tauri and UV Ceti stars simulteneously indicates nonstable stars. The population of flare stars in the Taurus Dark Cloud region is apparently as young as in Orion and Monoceros

Low-cost ARM Cortex-M0 Based TRNG for IoT Applications

Directory of Open Access Journals (Sweden)

Marek Laban

2018-03-01

Full Text Available The Internet of Things (IoT is one of perspective electronic sectors. In the near future a lot of common devices from a refrigerator to a door lock will be connected to the internet. Protection of the IoT devices should not be neglected. The device security is composed of many safety levels, where every countermeasure increases its robustness. The paper describes an implementation of a True Random Number Generator (TRNG used in many cryptographic algorithms and protocols. It is based on a modern low-cost and low-power STM32F050 ARM-M0 microcontroller, suitable especially for IoT applications. The main motivation for developing of such generator was its absence in lower members of microcontroller families. Integrated TRNG uses common features of the microcontroller, which may be portable across ARM-M0 architecture. A source of randomness is instability of internal RC oscillator, which is acquired using another faster clock and one timer. The paper follows a previous research, but using the modern microcontroller with proposed on-line embedded tests which are designed in order to be simple and effective.

Unexpected expansion of tRNA substrate recognition by the yeast m1G9 methyltransferase Trm10.

Science.gov (United States)

Swinehart, William E; Henderson, Jeremy C; Jackman, Jane E

2013-08-01

N-1 Methylation of the nearly invariant purine residue found at position 9 of tRNA is a nucleotide modification found in multiple tRNA species throughout Eukarya and Archaea. First discovered in Saccharomyces cerevisiae, the tRNA methyltransferase Trm10 is a highly conserved protein both necessary and sufficient to catalyze all known instances of m1G9 modification in yeast. Although there are 19 unique tRNA species that contain a G at position 9 in yeast, and whose fully modified sequence is known, only 9 of these tRNA species are modified with m1G9 in wild-type cells. The elements that allow Trm10 to distinguish between structurally similar tRNA species are not known, and sequences that are shared between all substrate or all nonsubstrate tRNAs have not been identified. Here, we demonstrate that the in vitro methylation activity of yeast Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity. Similarly, overexpression of Trm10 in yeast yields m1G9 containing tRNA species that are ordinarily unmodified in vivo. Thus, yeast Trm10 has a significantly broader tRNA substrate specificity than is suggested by the observed pattern of modification in wild-type yeast. These results may shed light onto the suggested involvement of Trm10 in other pathways in other organisms, particularly in higher eukaryotes that contain up to three different genes with sequence similarity to the single TRM10 gene in yeast, and where these other enzymes have been implicated in pathways beyond tRNA processing.

Patterns of failure and overall survival in patients with completely resected T3N0M0 nonsmall cell lung cancer

International Nuclear Information System (INIS)

Gould, P.M.; Bonner, J.A.; Sawyer, T.E.; Deschamps, C.; Foote, R.L.; Trastek, V.F.; Allen, M.S.; Pairolero, P.C.; Lange, C.; Li, H.

1997-01-01

Purpose/Objective: Previous studies of patients with surgically resected nonsmall cell lung cancer and chest wall invasion have shown conflicting results with respect to prognosis. Whether high risk subsets of the T3N0M0 population exist with respect to local, regional, and distant control as well as overall survival has been difficult to ascertain due to small numbers of patients in most reported series. Therefore, a review of patients with completely resected T3N0M0 nonsmall cell lung cancer was undertaken to analyze patient and tumor characteristics as well as surgical interventions that might influence patterns of failure and overall survival. Materials and Methods: A retrospective review was performed for all patients (91) with T3N0M0 nonsmall cell lung cancer who had undergone a complete resection between the years 1979 to 1993. The following potential prognostic factors were recorded from each patients history: tumor size, tumor location (bronchus vs. pleura vs. chestwall), tumor grade, histology, patient age, the use of adjuvant radiation therapy ((17(91)) patients received adjuvant therapy), and the type of surgical procedure performed (chestwall resection vs. extrapleural resection). The actuarial rates of freedom from local recurrence (FFLR), freedom from regional nodal recurrence (FFRR), freedom from distant recurrence (FFDR), and overall survival were calculated from the date of diagnosis by the method of Kaplan-Meier. Results: The following table illustrates two and five year outcomes: None of the patients, tumor, or treatment characteristics that were analyzed were associated with a significant influence on the four parameters outlined in the above table. Conclusion: Patients with completely resected T3N0M0 nonsmall cell lung cancer have a similar local control and overall survival irrespective of primary location, type of surgery performed, or use of adjuvant radiation therapy. Additionally, the tumor recurrence rate and overall survival found in

Meie ei ole ajaloota rahvas... : lisandusi M. Ojamaa, A. ja T. Varmase Eesti ajaloo saamisloole / Sirje Annist

Index Scriptorium Estoniae

Annist, Sirje

2004-01-01

Esimene eesti autorite koostatud Eesti ajaloo üldkäsitlus : M. Ojamaa, A. ja T. Varmas Viron historia, Porvoo, 1944. Tõlge eesti keelde 1946. aastal Stockholmis. Autoriteks pseudonüümide all kirjutanud kultuuritegelased : M. Ojamaa - Harri Moora, A. Varmas - August Annist, T. Varmas - Linda Annist

Belluse märk tähendab Põhjamaades kvaliteeti / Aarne Mäe

Index Scriptorium Estoniae

Mäe, Aarne, 1967-

2006-01-01

Üheksa aasta eest nelja mehega alustanud pehmet mööblit tootev Bellus Furniture on kasvamas paarisaja töölisega ettevõtteks, valmistudes Haljala tootmishoone järjekordseks juurdeehituseks. Lisa: Bellus Furniture. Vt. samas: Mööblimood pidevas muutumises

Rol de las células T regulatorias en esclerosis múltiple Role of T-regulatory cells in multiple sclerosis

Directory of Open Access Journals (Sweden)

Juan Ignacio Rojas

2010-02-01

Full Text Available La esclerosis múltiple (EM es una enfermedad inflamatoria autoinmune desmielinizante del sistema nervioso central (SNC. La mayoría de las enfermedades autoinmunes se originan por la activación anormal de la respuesta inflamatoria contra auto-antígenos (la mayoría de ellos desconocidos a la fecha como consecuencia de la pérdida de la tolerancia periférica. Las células T-regulatorias constituyen un grupo esencial de linfocitos T encargados del mantenimiento de la tolerancia periférica, la prevención de enfermedades autoinmunes y la limitación de enfermedades inflamatorias crónicas. Teniendo en cuenta la importancia de la tolerancia periférica, las células T-regulatorias serían componentes cruciales en el escenario fisiopatológico de los procesos autoinmunes, incluyendo la EM. El presente trabajo recopila los conocimientos actuales sobre la función de las células T-regulatorias en la EM, la enfermedad autoinmune desmielinizante del SNC más prevalente en los seres humanos.Multiple sclerosis (MS is an autoimmune inflammatory demyelinating disease of the central nervous system (CNS. Most of autoimmune diseases arise by an abnormal activation of the inflammatory response against self-antigens (most of them unknown up to date as a consequence of dysfunction in peripheral tolerance. Regulatory T-cells are essential for maintaining peripheral tolerance, preventing autoimmune diseases and limiting chronic inflammatory conditions. Based on that knowledge, T-regulatory cells have emerged as a key component of the physiopathology of autoimmune diseases including MS. This review compiles the current knowledge on the role and function of T-regulatory cells in MS, the most prevalent CNS autoimmune disease in humans.

AP1000"T"M plant modularization

International Nuclear Information System (INIS)

Cantarero L, C.; Demetri, K. J.; Quintero C, F. P.

2016-09-01

The AP1000"T"M plant is an 1100 M We pressurized water reactor (PWR) with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance and safety. Modules are used extensively in the design of the AP1000 plant nuclear island. The AP1000 plant uses modern, modular-construction techniques for plant construction. The design incorporates vendor-designed skids and equipment packages, as well as large, multi-ton structural modules and special equipment modules. Modularization allows traditionally sequential construction tasks to be completed simultaneously. Factory-built modules can be installed at the site in a planned construction schedule. The modularized AP1000 plant allows many more construction activities to proceed in parallel. This reduces plant construction calendar time, thus lowering the costs of plant financing. Furthermore, performing less work onsite significantly reduces the amount of skilled field-craft labor, which costs more than shop labor. In addition to labor cost savings, doing more welding and fabrication in a factory environment raises the quality of work, allowing more scheduling flexibility and reducing the amount of specialized tools required onsite. The site layout for the AP1000 plant has been established to support modular construction and efficient operations during construction. The plant layout is compact, using less space than previous conventional plant layouts. This paper provides and overview of the AP1000 plant modules with an emphasis on structural modules. Currently the Westinghouse AP1000 plant has four units under construction in China and four units under construction in the United States. All have shown successful fabrication and installation of various AP1000 plant modules. (Author)

Marie Underi luule : mõnest tõlkeprobleemist / Ilse Lehiste ; inglise k. tlk. Ene-Reet Soovik

Index Scriptorium Estoniae

Lehiste, Ilse, 1922-2010

2000-01-01

Orig.: Marie Under's poetry: some problems of translation. Varem ilmunud: Lituanus, 1983, nr. 3. Kaasautor: Rimvydas Shilbajoris. M. Underi luuletuse "Täiskuu" tekst eesti k., tõlge inglise keelde (tlk. I. Lehiste) ja reaalune tõlge inglise keelde, lk. 256-260

SAP is required for the development of innate phenotype in H2-M3-restricted CD8+ T cells1

Science.gov (United States)

Bediako, Yaw; Bian, Yao; Zhang, Hong; Cho, Hoonsik; Stein, Paul L.; Wang, Chyung-Ru

2012-01-01

H2-M3-restricted T cells have a pre-activated surface phenotype, rapidly expand and produce cytokines upon stimulation and as such, are classified as innate T cells. Unlike most innate T cells, M3-restricted T cells also express CD8αβ co-receptors and a diverse TCR repertoire: hallmarks of conventional MHC Ia-restricted CD8+ T cells. Although iNKT cells are also innate lymphocytes, they are selected exclusively on hematopoietic cells (HC), while M3-restricted T cells can be selected on either hematopoietic or thymic epithelial cells (TEC). Moreover, their phenotypes differ depending on what cells mediate their selection. Though there is a clear correlation between selection on HC and development of innate phenotype, the underlying mechanism remains unclear. SAP is required for the development of iNKT cells and mediates signals from SLAM receptors that are exclusively expressed on HC. Based on their dual selection pathway, M3-restricted T cells present a unique model for studying the development of innate T cell phenotype. Using both polyclonal and transgenic mouse models we demonstrate that while M3-restricted T cells are capable of developing in the absence of SAP, SAP is required for HC-mediated selection, development of pre-activated phenotype and heightened effector functions of M3-restricted T cells. These findings are significant because they directly demonstrate the need for SAP in HC-mediated acquisition of innate T cell phenotype and suggest that due to their SAP-dependent HC-mediated selection, M3-restricted T cells develop a pre-activated phenotype and an intrinsic ability to proliferate faster upon stimulation, allowing for an important role in the early response to infection. PMID:23041566

Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2

DEFF Research Database (Denmark)

Idorn, Manja; thor Straten, Eivind Per; Svane, Inge Marie

2016-01-01

Adoptive T-cell therapy based on the infusion of patient’s own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only...... infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred Tcells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting...

Is GDF5 gene promoter polymorphism +104T/C associated with osteoarthritis in the Eastern of Turkey population?

Science.gov (United States)

Tülüce, Y; Yildirim, I H; Özkol, H; Edi Z, L; Delen, V

2017-08-30

Osteoarthritis (OA) is the most common form of arthritis. Genetic factors have been shown to play important roles in the etiology of OA. The gene growth differentiation factor 5 (GDF5) has been implicated in skeletal development and joint morphogenesis in human and mice. A functional single nucleotide polymorphism (SNP) +104T/C in the 5'-UTR of GDF5 (rs143383) was reported to be associated with osteoarthritis susceptibility in Han Chinese and Japanese populations. Our objective was to assess whether this SNP was also associated with OA in the Eastern Turkey population.A total of 172 cases including 95 patients with idiopathic OA and 77 control cases were recruited into the study. DNA samples were extracted from peripheral blood lymphocytes of all cases by using salting out method. The +104T/C polymorphism was genotyped by PCR-RFLP method. In terms of genotype comparison there wasn't any correlation between patient and control groups. Frequency of C allele was found to be higher in-patient group than control group and statistical analysis showed a poor correlation in allele frequencies of the +104T/C SNP of GDF5 gene between cases and controls (p<0.05). Significant correlation between GDF5 and OA has been reported in Asian population, especially T alleles were found in higher frequencies and related to OA.  Our study did not confirm this association and also in term of T allele. Interestingly, we found higher frequency of C allele in patient group than control group and our results are compatible with the study carried out in Greek population.

Being WISE. I. Validating stellar population models and M */L ratios at 3.4 and 4.6 μm

International Nuclear Information System (INIS)

Norris, Mark A.; Meidt, Sharon; Van de Ven, Glenn; Schinnerer, Eva; Groves, Brent; Querejeta, Miguel

2014-01-01

Using data from the Wide-field Infrared Survey Explorer mission, we have measured near infra-red (NIR) photometry of a diverse sample of dust-free stellar systems (globular clusters, dwarf and giant early-type galaxies) which have metallicities that span the range -2.2 < [Fe/H] (dex) < 0.3. This dramatically increases the sample size and broadens the metallicity regime over which the 3.4 (W1) and 4.6 μm (W2) photometry of stellar populations have been examined. We find that the W1 – W2 colors of intermediate and old (>2 Gyr) stellar populations are insensitive to the age of the stellar population, but that the W1 – W2 colors become bluer with increasing metallicity, a trend not well reproduced by most stellar population synthesis (SPS) models. In common with previous studies, we attribute this behavior to the increasing strength of the CO absorption feature located in the 4.6 μm bandpass with metallicity. Having used our sample to validate the efficacy of some of the SPS models, we use these models to derive stellar mass-to-light ratios in the W1 and W2 bands. Utilizing observational data from the SAURON and ATLAS3D surveys, we demonstrate that these bands provide extremely simple, yet robust stellar mass tracers for dust free older stellar populations that are freed from many of the uncertainties common among optical estimators.

Two tandem RNase III cleavage sites determine betT mRNA stability in response to osmotic stress in Escherichia coli.

Directory of Open Access Journals (Sweden)

Minji Sim

Full Text Available While identifying genes regulated by ribonuclease III (RNase III in Escherichia coli, we observed that steady-state levels of betT mRNA, which encodes a transporter mediating the influx of choline, are dependent on cellular concentrations of RNase III. In the present study, we also observed that steady-state levels of betT mRNA are dependent on RNase III activity upon exposure to osmotic stress, indicating the presence of cis-acting elements controlled by RNase III in betT mRNA. Primer extension analyses of betT mRNA revealed two tandem RNase III cleavage sites in its stem-loop region, which were biochemically confirmed via in vitro cleavage assays. Analyses of cleavage sites suggested the stochastic selection of cleavage sites by RNase III, and mutational analyses indicated that RNase III cleavage at either site individually is insufficient for efficient betT mRNA degradation. In addition, both the half-life and abundance of betT mRNA were significantly increased in association with decreased RNase III activity under hyper-osmotic stress conditions. Our findings demonstrate that betT mRNA stability is controlled by RNase III at the post-transcriptional level under conditions of osmotic stress.

Coverage and Capacity Analysis of LTE-M and NB-IoT in a Rural Area

DEFF Research Database (Denmark)

Lauridsen, Mads; Kovács, István; Mogensen, Preben Elgaard

2016-01-01

equipped with either of the newly standardized technologies. The study is made for a site specific network deployment of a Danish operator, and the simulation is calibrated using drive test measurements. The results show that LTE-M can provide coverage for 99.9% of outdoor and indoor devices, if the latter......The 3GPP has introduced the LTE-M and NB-IoT User Equipment categories and made amendments to LTE release 13 to support the cellular Internet of Things. The contribution of this paper is to analyze the coverage probability, the number of supported devices, and the device battery life in networks...... is experiencing 10 dB additional loss. However, for deep indoor users NB-IoT is required and provides coverage for about 95% of the users. The cost is support for more than 10 times fewer devices and a 2-6 times higher device power consumption. Thus both LTE-M and NB-IoT provide extended support for the cellular...

Improved supercapacitor performance of MnO2-electrospun carbon nanofibers electrodes by mT magnetic field

Science.gov (United States)

Zeng, Zheng; Liu, Yiyang; Zhang, Wendi; Chevva, Harish; Wei, Jianjun

2017-08-01

This work reports on a finding of mT magnetic field induced energy storage enhancement of MnO2-based supercapacitance electrodes (magneto-supercapacitor). Electrodes with MnO2 electrochemically deposited at electrospun carbon nanofibers (ECNFs) film are studied by cyclic voltammetry (CV), galvanostatic charge/discharge, electrochemical impedance spectroscopy (EIS), and life cycle stability tests in the presence/absence of milli-Tesla (mT) magnetic fields derived by Helmholtz coils. In the presence of a 1.34 mT magnetic field, MnO2/ECNFs shows a magneto-enhanced capacitance of 141.7 F g-1 vs. 119.2 F g-1 (∼19% increase) with absence of magnetic field at a voltage sweeping rate of 5 mV s-1. The mechanism of the magneto-supercapacitance is discussed and found that the magnetic susceptibility of the MnO2 significantly improves the electron transfer of a pseudo-redox reaction of Mn(IV)/Mn(III) at the electrode, along with the magnetic field induced impedance effect, which may greatly enhance the interface charge density, facilitate electrolyte transportation, and improve the efficiency of cation intercalation/de-intercalation of the pseudocapacitor under mT-magnetic field exposure, resulting in enhancement of energy storage capacitance and longer charge/discharge time of the MnO2/ECNFs electrode without sacrificing its life cycle stability.

Use of I.G.R.T. for prostate cancers (O.B.I.-C.B.C.T. VarianTM, ExacTrac BrainLABTM and M.V.C.T. Tomo-therapy)

International Nuclear Information System (INIS)

Delpon, G.; Llagostera, C.; Lisbona, A.; Le Blanc, M.; Rio, E.; Supiot, S.; Mahe, M.A.

2009-01-01

Introduction: The aim of this work was to report the experience of image-guided radiotherapy at the C.L.C.C. Nantes-Atlantique using three repositioning imaging devices, the ExacTrac (BrainLABTM), the on-board imager cone beam computed tomography (O.B.I.-C.B.C.T.) (Varian TM ) and the M.V.C.T. (Tomotherapy Inc TM ), in the case of prostate external radiotherapy.Material and methods: For each linac and its imaging device, a treatment plan was described. Moreover, studies concerning calculated shifts after imaging sessions were achieved. Using ExacTrac, for eight patients, a study compared daily shifts based on bony anatomy or on implanted markers. Considering mean values of displacements over a course of radiotherapy, dosimetric impact was evaluated. With the O.B.I.-C.B.C.T., two imaging modalities were used, kV-kV (0. and 270.) and C.B.C.T.. Up to now, whatever the images, displacements were calculated using the bony anatomy. For both modalities and for 26 patients, shifts were compared. Since the beginning of the Tomotherapy HiArt use, mega voltage cone tomography (M.V.C.T.) was performed for each session of each patient. For 12 patients, mean displacements were calculated after five fractions. Then the deviations to those values were calculated. This was done to show the relevance of daily M.V.C.T.. Results and conclusion: This work allows us to report the use of three repositioning imaging devices in the radiotherapy department. At least: they provide an efficient positioning tool. And they let us see the future radiotherapy which would probably be the dose-guided radiotherapy. (N.C.)

44 CFR 206.377 - Loan repayment.

Science.gov (United States)

2010-10-01

... interest amount due will be computed separately for each Treasury disbursement as follows: I = P X R X T, where I = the amount of simple interest, P = the principal amount disbursed; R = the interest rate of... Assistant Administrator for the Disaster Assistance Directorate. Interest will accrue on outstanding cash...

Virus-specific regulatory T cells ameliorate encephalitis by repressing effector T cell functions from priming to effector stages.

Directory of Open Access Journals (Sweden)

Jingxian Zhao

2014-08-01

Full Text Available Several studies have demonstrated the presence of pathogen-specific Foxp3+ CD4 regulatory T cells (Treg in infected animals, but little is known about where and how these cells affect the effector T cell responses and whether they are more suppressive than bulk Treg populations. We recently showed the presence of both epitope M133-specific Tregs (M133 Treg and conventional CD4 T cells (M133 Tconv in the brains of mice with coronavirus-induced encephalitis. Here, we provide new insights into the interactions between pathogenic Tconv and Tregs responding to the same epitope. M133 Tregs inhibited the proliferation but not initial activation of M133 Tconv in draining lymph nodes (DLN. Further, M133 Tregs inhibited migration of M133 Tconv from the DLN. In addition, M133 Tregs diminished microglia activation and decreased the number and function of Tconv in the infected brain. Thus, virus-specific Tregs inhibited pathogenic CD4 T cell responses during priming and effector stages, particularly those recognizing cognate antigen, and decreased mortality and morbidity without affecting virus clearance. These cells are more suppressive than bulk Tregs and provide a targeted approach to ameliorating immunopathological disease in infectious settings.

Põhiõiguste mõiste ja tähtsus õigussüsteemis / Madis Ernits

Index Scriptorium Estoniae

Ernits, Madis, 1971-

1996-01-01

Põhiõiguse, põhivabaduse ja põhikohustuse mõiste, põhiõiguste liigitus, ajaline ja ruumiline kehtivus, suhe rahvusvaheliste konventsioonidega ning tähtsus õigussüsteemis toetudes Saksa põhiseaduse dogmaatikale

Scavenging of Tc(V) formed by I.T. in 95mTcO4- solutions

International Nuclear Information System (INIS)

Ianoz, E.; Colin, M.; Kosinski, M.

1988-01-01

The chemical effects of the I.T. of 95m Tc in 95m TcO 4 - have been studied in chelating ligand solutions. At high pH and at high concentration of 1,4,8,11-tetraazacyclotetradecane and 1,4-dithia-8, 11-diazacyclotetradecane, the retention of 95g Tc is about 20% and the unretained 95g Tc appears preponderantly (ca. 73%) as [TcO 2 L] + complexes. In glucoheptonate solution, the 95g Tc retention remains practically the same (22%) but the unretained 95g Tc is found in high proportion (73%) as [TcObis(glucoheptonate)] - . The added ligands are very good scavengers for 95g Tc(V). A comparison is made between 95g Tc species formed by the I.T. in 95m TcO 4 - and 99m Tc species resulting from the chemical reduction of 99m TcO 4 - . (orig.)

Development of new mouse lung tumor models expressing EGFR T790M mutants associated with clinical resistance to kinase inhibitors.

Science.gov (United States)

Regales, Lucia; Balak, Marissa N; Gong, Yixuan; Politi, Katerina; Sawai, Ayana; Le, Carl; Koutcher, Jason A; Solit, David B; Rosen, Neal; Zakowski, Maureen F; Pao, William

2007-08-29

The EGFR T790M mutation confers acquired resistance to kinase inhibitors in human EGFR mutant lung adenocarcinoma, is occasionally detected before treatment, and may confer genetic susceptibility to lung cancer. To study further its role in lung tumorigenesis, we developed mice with inducible expression in type II pneumocytes of EGFR(T790M) alone or together with a drug-sensitive L858R mutation. Both transgenic lines develop lung adenocarcinomas that require mutant EGFR for tumor maintenance but are resistant to an EGFR kinase inhibitor. EGFR(L858R+T790M)-driven tumors are transiently targeted by hsp90 inhibition. Notably, EGFR(T790M)-expressing animals develop tumors with longer latency than EGFR(L858R+T790M)-bearing mice and in the absence of additional kinase domain mutations. These new mouse models of mutant EGFR-dependent lung adenocarcinomas provide insight into clinical observations. The models should also be useful for developing improved therapies for patients with lung cancers harboring EGFR(T790M) alone or in conjunction with drug-sensitive EGFR kinase domain mutations.

Development of new mouse lung tumor models expressing EGFR T790M mutants associated with clinical resistance to kinase inhibitors.

Directory of Open Access Journals (Sweden)

Lucia Regales

2007-08-01

Full Text Available The EGFR T790M mutation confers acquired resistance to kinase inhibitors in human EGFR mutant lung adenocarcinoma, is occasionally detected before treatment, and may confer genetic susceptibility to lung cancer.To study further its role in lung tumorigenesis, we developed mice with inducible expression in type II pneumocytes of EGFR(T790M alone or together with a drug-sensitive L858R mutation. Both transgenic lines develop lung adenocarcinomas that require mutant EGFR for tumor maintenance but are resistant to an EGFR kinase inhibitor. EGFR(L858R+T790M-driven tumors are transiently targeted by hsp90 inhibition. Notably, EGFR(T790M-expressing animals develop tumors with longer latency than EGFR(L858R+T790M-bearing mice and in the absence of additional kinase domain mutations.These new mouse models of mutant EGFR-dependent lung adenocarcinomas provide insight into clinical observations. The models should also be useful for developing improved therapies for patients with lung cancers harboring EGFR(T790M alone or in conjunction with drug-sensitive EGFR kinase domain mutations.

Mobilā mārketinga izmantošana un tā attīstības perspektīvas Latvijā

OpenAIRE

Treija, Solveiga

2014-01-01

Solveiga Treija. Maģistra darbs „Mobilā mārketinga izmantošana un tā attīstības perspektīvas Latvijā.” Maģistra darba mērķis ir, pamatojoties uz teorijas atziņām par mobilo mārketingu, mobilo ierīču lietotāju aptaujas rezultātiem un padziļināto interviju ar nozares speciālistiem rezultātiem, novērtēt mobilā mārketinga izmantošanu Latvijā, kā arī izstrādāt priekšlikumus mobilā mārketinga attīstībai uzņēmējdarbībā Latvijā. Pirmajā nodaļā pētīta mobilā mārketinga būtība, galvenie rīki un ...

Possible Intervals for T- and M-Orders of Solutions of Linear Differential Equations in the Unit Disc

Directory of Open Access Journals (Sweden)

Martin Chuaqui

2011-01-01

f(k+ak-1(zf(k-1+⋯+a1(zf′+a0(zf=0 with polynomial coefficients. In the present paper, it is shown by an example that a unit disc counterpart of such finite set does not contain all possible T- and M-orders of solutions, with respect to Nevanlinna characteristic and maximum modulus, if the coefficients are analytic functions belonging either to weighted Bergman spaces or to weighted Hardy spaces. In contrast to a finite set, possible intervals for T- and M-orders are introduced to give detailed information about the growth of solutions. Finally, these findings yield sharp lower bounds for the sums of T- and M-orders of functions in the solution bases.

Mechanisms of Recovering Low Cycle Fatigue Damage in Incoloy 901.

Science.gov (United States)

1979-01-01

ZAP. 0665 0374 19A% DATA : 19AE MPS 15 0666 2375 F2e4 JMP .IDF r e667 e376 9 110 LSI ZAP 668 e377 E2?E LOX F,12 ASSURE X- PEG !0SITIVE FOR LSI 0669 0378...NC(MC) 0( 3350 PRIN4T 22,N4TDELTELC(M),DLTPLC(MC,SIGC(MC)o , 03360 A~1~𔃾C..,TP(M.T~4l~lC) -003370 230 22 VORMAT (12, IT1,F6.5,T2,F6.5,T30,F7.2,T42

Experimental study on the losses and magnetic fields of coils inside a metallic liner for T.T.M.P. heating application

International Nuclear Information System (INIS)

Brossaud, J.; Perin, J.P.; Rey, G.

1978-11-01

The purpose of this work is the improvement of the T.T.M.P. configuration in the Tokamak PETULA in the case of one or three wavelengths. The variation of the magnetic field distribution and the electrical characteristics coils (R//,Q, L) inside a metallic liner have been studied as functions of: position and number of coils, radius and width of coils, number of wavelengths along the axis of torus [fr

Mājas lapas loma mārketinga komunikācijā: veikalu tīkla "Biotēka" piemērs

OpenAIRE

Drizļionoka, Diāna

2010-01-01

Autores Diānas Drizļionokas izstrādātā bakalaura darba temats ir „Mājas lapas loma mārketinga komunikācijā: veikalu tīkla „Biotēka” piemērs” . Darba ietvaros veiktā pētījuma mērķis noteikt mājas lapas lomu mazumtirdzniecības veikalu tīkla „Biotēka” mārketinga komunikācijā. Darbā izstrādē autore balstās uz Filipa Kotlera (Philip Kotler) teorētiskajām nostādnēm par mārketingu, Davīda Čefija (David Chaffey) teorētiskajām nostādnēm par interneta mārketingu un mājas lapu, Tomasa Pauela (Thomas Pow...

Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking

NARCIS (Netherlands)

Sinclair, Linda V.; Finlay, David; Feijoo, Carmen; Cornish, Georgina H.; Gray, Alex; Ager, Ann; Okkenhaug, Klaus; Hagenbeek, Thijs J.; Spits, Hergen; Cantrell, Doreen A.

2008-01-01

Phosphatidylinositol-3-OH kinase (PI(3)K) and the nutrient sensor mTOR are evolutionarily conserved regulators of cell metabolism. Here we show that PI(3)K and mTOR determined the repertoire of adhesion and chemokine receptors expressed by T lymphocytes. The key lymph node-homing receptors CD62L

M13 multiple stellar populations seen with the eyes of Strömgren photometry

Science.gov (United States)

Savino, A.; Massari, D.; Bragaglia, A.; Dalessandro, E.; Tolstoy, E.

2018-03-01

We present a photometric study of M13 multiple stellar populations over a wide field of view, covering approximately 6.5 half-light radii, using archival Isaac Newton Telescope observations to build an accurate multiband Strömgren catalogue. The use of the Strömgren index cy permits us to separate the multiple populations of M13 on the basis of their position on the red giant branch. The comparison with medium and high resolution spectroscopic analysis confirms the robustness of our selection criterion. To determine the radial distribution of stars in M13, we complemented our data set with Hubble Space Telescope observations of the cluster core, to compensate for the effect of incompleteness affecting the most crowded regions. From the analysis of the radial distributions, we do not find any significant evidence of spatial segregation. Some residuals may be present in the external regions where we observe only a small number of stars. This finding is compatible with the short dynamical time-scale of M13 and represents, to date, one of the few examples of fully spatially mixed multiple populations in a massive globular cluster.

Efficiency of Floristic and Molecular Markers to Determine Diversity in Iranian Populations of T. boeoticum

OpenAIRE

M. R. Naghavi; M. Maleki; S. F. Tabatabaei

2009-01-01

In order to study floristic and molecular classification of common wild wheat (Triticum boeoticum Boiss.), an analysis was conducted on populations of the Triticum boeoticum collected from different regions of Iran. Considering all floristic compositions of habitats, six floristic groups (syntaxa) within the populations were identified. A high level of variation of T. boeoticum also detected using SSR markers. Our results showed that molecular method confirmed the groupin...

THE DEEPEST HUBBLE SPACE TELESCOPE COLOR-MAGNITUDE DIAGRAM OF M32. EVIDENCE FOR INTERMEDIATE-AGE POPULATIONS

International Nuclear Information System (INIS)

Monachesi, Antonela; Trager, Scott C.; Lauer, Tod R.; Mighell, Kenneth J.; Freedman, Wendy; Dressler, Alan; Grillmair, Carl

2011-01-01

We present the deepest optical color-magnitude diagram (CMD) to date of the local elliptical galaxy M32. We have obtained F435W and F555W photometries based on Hubble Space Telescope (HST) Advanced Camera for Surveys/High-Resolution Channel images for a region 110'' from the center of M32 (F1) and a background field (F2) about 320'' away from M32 center. Due to the high resolution of our Nyquist-sampled images, the small photometric errors, and the depth of our data (the CMD of M32 goes as deep as F435W ∼ 28.5 at 50% completeness level), we obtain the most detailed resolved photometric study of M32 yet. Deconvolution of HST images proves to be superior than other standard methods to derive stellar photometry on extremely crowded HST images, as its photometric errors are ∼2x smaller than other methods tried. The location of the strong red clump in the CMD suggests a mean age between 8 and 10 Gyr for [Fe/H] = -0.2 dex in M32. We detect for the first time a red giant branch bump and an asymptotic giant branch (AGB) bump in M32 which, together with the red clump, allow us to constrain the age and metallicity of the dominant population in this region of M32. These features indicate that the mean age of M32's population at ∼2' from its center is between 5 and 10 Gyr. We see evidence of an intermediate-age population in M32 mainly due to the presence of AGB stars rising to M F555W ∼ -2.0. Our detection of a blue component of stars (blue plume) may indicate for the first time the presence of a young stellar population, with ages of the order of 0.5 Gyr, in our M32 field. However, it is likely that the brighter stars of this blue plume belong to the disk of M31 rather than to M32. The fainter stars populating the blue plume indicate the presence of stars not younger than 1 Gyr and/or BSSs in M32. The CMD of M32 displays a wide color distribution of red giant branch stars indicating an intrinsic spread in metallicity with a peak at [Fe/H] ∼ -0.2. There is not a

Úvahy ke statusovým limitům smluvní podstaty korporace

Czech Academy of Sciences Publication Activity Database

Havel, Bohumil

2014-01-01

Roč. 97, č. 4 (2014), s. 377-382 ISSN 0032-6984 Institutional support: RVO:68378122 Keywords : commercial companies * corporations * mandatory and supplementary legal rules Subject RIV: AG - Legal Sciences

TRPV6 exhibits unusual patterns of polymorphism and divergence in worldwide populations.

Science.gov (United States)

Akey, Joshua M; Swanson, Willie J; Madeoy, Jennifer; Eberle, Michael; Shriver, Mark D

2006-07-01

A striking footprint of positive selection was recently identified on chromosome 7q34-35 that spans at least 115 kb and encompasses four known genes (KEL, TRPV5, TRPV6, EPHB6). The signature of selection was observed in only one of the two populations analyzed suggesting the action of geographically restricted selective pressures. However, as only two populations were analyzed, it remains unknown whether the signature of selection extends to additional populations. To address this issue and begin to dissect the evolutionary history of this region in more detail, we performed an in-depth population genetic analysis on TRPV6, which is a calcium-permeable ion channel thought to mediate the rate-limiting step of dietary calcium absorption. We demonstrate that the rate of TRPV6 protein evolution is significantly accelerated in the human lineage, but only for a haplotype defined by three non-synonymous SNPs (C157R, M378V and M681T) that are nearly fixed for the derived alleles in non-African populations. Interestingly, we found that these three non-synonymous SNPs have high posterior probabilities for being targets of positive selection and are therefore strong candidates for mediating the population-specific signatures of selection in this region. In addition, we resequenced the exons corresponding to the C157R, M378V and M681T polymorphisms in 90 geographically diverse individuals and characterized their global allele frequency distribution by genotyping them in 1064 individuals from 52 populations. These data strongly suggest that the TRPV6 haplotype defined by the derived alleles at C157R, M378V and M681T conferred a selective advantage that varied spatially, and perhaps temporally, during human history.

Metastability exchange optical pumping in 3He gas up to 30 mT. Efficiency measurements and evidence of laser-induced nuclear relaxation

International Nuclear Information System (INIS)

Batz, Marion

2011-01-01

Advances in metastability exchange optical pumping (MEOP) of 3 He at high laser powers, with its various applications, but also at high gas pressures p 3 and high magnetic field strengths B, have provided strong motivation for revisiting the understanding and for investigating the limitations of this powerful technique. For this purpose, we present systematic experimental and theoretical studies of efficiency and of relaxation mechanisms in B≤30 mT and p 3 =0.63-2.45 mbar. 3 He nuclear polarisation is measured by light absorption in longitudinal configuration where weak light beams at 1083 nm parallel to magnetic field and cell axis with opposite circular polarisations are used to probe the distribution of populations in the metastable state. This method is systematically tested to evaluate potential systematic biases and is shown to be reliable for the study of OP dynamics despite the redistribution of populations by OP light. Nuclear polarisation loss associated to the emission of polarised light by the plasma discharge used for MEOP is found to decrease above 10 mT, as expected, due to hyperfine decoupling in highly excited states. However, this does not lead to improved MEOP efficiency at high laser power. We find clear evidence of additional laser-induced relaxation instead. The strong OP-enhanced polarisation losses, currently limiting MEOP performances, are quantitatively investigated using an angular momentum budget approach and a recently developed comprehensive model that describes the combined effects of OP, ME and relaxation, validated by comparison to experimental results.

Immunological quality and performance of tumor vessel-targeting CAR-T cells prepared by mRNA-EP for clinical research.

Science.gov (United States)

Inoo, Kanako; Inagaki, Ryo; Fujiwara, Kento; Sasawatari, Shigemi; Kamigaki, Takashi; Nakagawa, Shinsaku; Okada, Naoki

2016-01-01

We previously reported that tumor vessel-redirected T cells, which were genetically engineered with chimeric antigen receptor (CAR) specific for vascular endothelial growth factor receptor 2 (VEGFR2), demonstrated significant antitumor effects in various murine solid tumor models. In the present study, we prepared anti-VEGFR2 CAR-T cells by CAR-coding mRNA electroporation (mRNA-EP) and analyzed their immunological characteristics and functions for use in clinical research. The expression of anti-VEGFR2 CAR on murine and human T cells was detected with approximately 100% efficiency for a few days, after peaking 6-12 hours after mRNA-EP. Triple transfer of murine anti-VEGFR2 CAR-T cells into B16BL6 tumor-bearing mice demonstrated an antitumor effect comparable to that for the single transfer of CAR-T cells engineered with retroviral vector. The mRNA-EP did not cause any damage or defects to human T-cell characteristics, as determined by viability, growth, and phenotypic parameters. Additionally, two kinds of human anti-VEGFR2 CAR-T cells, which expressed different CAR construction, differentiated to effector phase with cytokine secretion and cytotoxic activity in antigen-specific manner. These results indicate that our anti-VEGFR2 CAR-T cells prepared by mRNA-EP have the potential in terms of quality and performance to offer the prospect of safety and efficacy in clinical research as cellular medicine.

Immunological quality and performance of tumor vessel-targeting CAR-T cells prepared by mRNA-EP for clinical research

Directory of Open Access Journals (Sweden)

Kanako Inoo

2016-01-01

Full Text Available We previously reported that tumor vessel-redirected T cells, which were genetically engineered with chimeric antigen receptor (CAR specific for vascular endothelial growth factor receptor 2 (VEGFR2, demonstrated significant antitumor effects in various murine solid tumor models. In the present study, we prepared anti-VEGFR2 CAR-T cells by CAR-coding mRNA electroporation (mRNA-EP and analyzed their immunological characteristics and functions for use in clinical research. The expression of anti-VEGFR2 CAR on murine and human T cells was detected with approximately 100% efficiency for a few days, after peaking 6–12 hours after mRNA-EP. Triple transfer of murine anti-VEGFR2 CAR-T cells into B16BL6 tumor-bearing mice demonstrated an antitumor effect comparable to that for the single transfer of CAR-T cells engineered with retroviral vector. The mRNA-EP did not cause any damage or defects to human T-cell characteristics, as determined by viability, growth, and phenotypic parameters. Additionally, two kinds of human anti-VEGFR2 CAR-T cells, which expressed different CAR construction, differentiated to effector phase with cytokine secretion and cytotoxic activity in antigen-specific manner. These results indicate that our anti-VEGFR2 CAR-T cells prepared by mRNA-EP have the potential in terms of quality and performance to offer the prospect of safety and efficacy in clinical research as cellular medicine.

ON OPERATION OF 740 M LONG FREIGHT TRAINS ON CZECH TEN-T RAILWAY NETWORK

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Michal Drábek

2016-09-01

Full Text Available Regulation (EU No 1315/2013 defines actual scope of core and comprehensive TEN-T network, including both networks for railway freight transport. For the core network, possibility to operate 740 m long freight trains is required. The aim of this paper is to analyse availability of appropriate overtaking tracks for 740 m long freight trains. Due to ETCS braking curves and odometry, such trains, after ETCS implementation, will require 780-800 m long overtaking tracks. For practical reasons (e.g. bypass lines, whole Czech railway TEN-T network is analysed. The overtaking track, whose occupation means influence on scheduled traffic or threat to boarding passengers, are excluded. The data was collected from station schemes from Collection of Official Requisites for 2015/16 Timetable, issued by SŽDC, Czech state Infrastructure Manager. Most of appropriate tracks are over 800 m long, but their density in the network and in particular directions varies considerably. For freight traffic, gradient of the line is important, so in the resulting figure, there are marked significant peaks for particular lines as well. Czech TEN-T lines are further segmented on the basis of number of tracks and their traffic character. Then, specific issues on overtaking or crossing of 740 m long freight trains are discussed. As a conclusion, for long-term development of Czech TEN-T lines, targeted investment is recommended not only for passenger railway, but also for freight railway. An attractive capacity offer for railway undertakings, which can stimulate freight traffic on European Rail freight corridors, can be represented by network-bound periodic freight train paths with suitable long overtaking tracks outside bottlenecks. After the overtaking by passenger trains, a freight train should run without stop through large node station or a bottleneck area. Before the sections with high gradients, coupling of additional locomotives should be connected with the overtaking

Bayesian population structure analysis reveals presence of phylogeographically specific sublineages within previously ill-defined T group of Mycobacterium tuberculosis.

Directory of Open Access Journals (Sweden)

Yann Reynaud

Full Text Available Mycobacterium tuberculosis genetic structure, and evolutionary history have been studied for years by several genotyping approaches, but delineation of a few sublineages remains controversial and needs better characterization. This is particularly the case of T group within lineage 4 (L4 which was first described using spoligotyping to pool together a number of strains with ill-defined signatures. Although T strains were not traditionally considered as a real phylogenetic group, they did contain a few phylogenetically meaningful sublineages as shown using SNPs. We therefore decided to investigate if this observation could be corroborated using other robust genetic markers. We consequently made a first assessment of genetic structure using 24-loci MIRU-VNTRs data extracted from the SITVIT2 database (n = 607 clinical isolates collected in Russia, Albania, Turkey, Iraq, Brazil and China. Combining Minimum Spanning Trees and Bayesian population structure analyses (using STRUCTURE and TESS softwares, we distinctly identified eight tentative phylogenetic groups (T1-T8 with a remarkable correlation with geographical origin. We further compared the present structure observed with other L4 sublineages (n = 416 clinical isolates belonging to LAM, Haarlem, X, S sublineages, and showed that 5 out of 8 T groups seemed phylogeographically well-defined as opposed to the remaining 3 groups that partially mixed with other L4 isolates. These results provide with novel evidence about phylogeographically specificity of a proportion of ill-defined T group of M. tuberculosis. The genetic structure observed will now be further validated on an enlarged worldwide dataset using Whole Genome Sequencing (WGS.

Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population.

Directory of Open Access Journals (Sweden)

Xuefeng Wang

Full Text Available OBJECTIVE: The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627 and -511T/C (rs16944 polymorphisms and the preeclampsia (PE, and analyze the Linkage disequilibrium (LD and haplotype frequency of the two polymorphism loci. METHODS: Polymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(X2 tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2. RESULTS: Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: X2 = 11.478, P = 0.003; For IL-1β-511T/C: X2 = 9.687, P = 0.008. LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D' = 0.92, r2 = 0.79. Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016. CONCLUSION: Our results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE.

MTHFR C677T genotype and cardiovascular risk in a general population without mandatory folic acid fortification

DEFF Research Database (Denmark)

Husemoen, Lise Lotte N; Skaaby, Tea; Jørgensen, Torben

2014-01-01

Meta-analyses have suggested an effect of MTHFR C677T genotype (rs1801133), a proxy for blood total homocysteine, on cardiovascular disease (CVD) in populations with low population dietary folate. The aim was to examine the association and effect modification by serum folate and vitamin B12 levels...

Genome Sequence of Selenium-Solubilizing Bacterium Caulobacter vibrioides T5M6

DEFF Research Database (Denmark)

Wang, Yihua; Qin, Yanan; Kot, Witold

2016-01-01

Caulobacter vibrioides T5M6 is a Gram-negative strain that strongly solubilizes selenium (Se) mineral into Se(IV) and was isolated from a selenium mining area in Enshi, southwest China. This strain produces the phytohormone IAA and promotes plant growth. Here we present the genome of this strain...

Enhancing the Production of D-Mannitol by an Artificial Mutant of Penicillium sp. T2-M10.

Science.gov (United States)

Duan, Rongting; Li, Hongtao; Li, Hongyu; Tang, Linhuan; Zhou, Hao; Yang, Xueqiong; Yang, Yabin; Ding, Zhongtao

2018-05-26

D-Mannitol belongs to a linear polyol with six-carbon and has indispensable usage in medicine and industry. In order to obtain more efficient D-mannitol producer, this study has screened out a stable mutant Penicillium sp. T2-M10 that was isolated from the initial D-mannitol-produced strain Penicillium sp.T2-8 via UV irradiation as well as nitrosoguanidine (NTG) induction. The mutant had a considerable enhancement in yield of D-mannitol based on optimizing fermentation. The production condition was optimized as the PDB medium with 24 g/L glucose for 9 days. The results showed that the production of D-mannitol from the mutant strain T2-M10 increased 125% in contrast with the parental strain. Meanwhile, the fact that D-mannitol is the main product in the mutant simplified the process of purification. Our finding revealed the potential value of the mutant strain Penicillium sp. T2-M10 to be a D-mannitol-producing strain.

Concurrent chemoradiotherapy for T4 and/or M1 LYM squamous cell carcinoma of the esophagus

Energy Technology Data Exchange (ETDEWEB)

Miyata, Yoshinori; Seki, Shigeki [Saku Central Hospital, Usuda, Nagano (Japan); Ohtsu, Atsushi; Kaneko, Kazuhiro; Nakamura, Akira

2000-07-01

A phase II study was conducted to investigate the efficacy and feasibility of chemoradiotherapy for locally advanced carcinoma of the esophagus. Fifty-four patients with clinical T4 and/or M1 LYM squamous cell carcinoma of the esophagus were enrolled. Patients received protracted infusions of fluorouracil 400 mg/m{sup 2}/24 hours on days 1 to 5 and 8 to 12, 2-hour infusions of cisplatin 40 mg/m{sup 2} on days 1 and 8, and concurrent radiotherapy at a dose of 30 Gy in 15 fractions over 3 weeks. Filgrastim was prophylactically administered to 35 patients. This schedule was repeated twice every 5 weeks, for a total radiation dose of 60 Gy followed by two courses of fluorouracil (800 mg/m{sup 2}/24 hours for 5 days) and cisplatin (80 mg/m{sup 2} on day 1). There were 36 patients with T4 disease and 33 with M1 LYM. Forty-nine patients (91%) completed the chemoradiotherapy segment. The 18 patients (33%) who achieved a complete response included nine (25%) of the 36 with T4 disease and nine (50%) of the 18 with non-T4 disease. Major toxicities were leukopenia and esophagitis; there were four (7%) treatment-related deaths. Prophylactic filgrastim reduced the incidence of grade 3 or worse leukopenia without improving dose-intensity or response. With a median follow-up duration of 43 months, median survival time was 9 months. The 3-year survival rate was 23%. Despite its significant toxicity, this combined modality seemed to have curative potential even in cases of locally advanced carcinoma of the esophagus. (author)

T Cell Epitope Immunotherapy Induces a CD4+ T Cell Population with Regulatory Activity

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Verhoef Adrienne

2005-01-01

Full Text Available Background Synthetic peptides, representing CD4+ T cell epitopes, derived from the primary sequence of allergen molecules have been used to down-regulate allergic inflammation in sensitised individuals. Treatment of allergic diseases with peptides may offer substantial advantages over treatment with native allergen molecules because of the reduced potential for cross-linking IgE bound to the surface of mast cells and basophils. Methods and Findings In this study we address the mechanism of action of peptide immunotherapy (PIT in cat-allergic, asthmatic patients. Cell-division-tracking dyes, cell-mixing experiments, surface phenotyping, and cytokine measurements were used to investigate immunomodulation in peripheral blood mononuclear cells (PBMCs after therapy. Proliferative responses of PBMCs to allergen extract were significantly reduced after PIT. This was associated with modified cytokine profiles generally characterised by an increase in interleukin-10 and a decrease in interleukin-5 production. CD4+ cells isolated after PIT were able to actively suppress allergen-specific proliferative responses of pretreatment CD4neg PBMCs in co-culture experiments. PIT was associated with a significant increase in surface expression of CD5 on both CD4+ and CD8+ PBMCs. Conclusion This study provides evidence for the induction of a population of CD4+ T cells with suppressor/regulatory activity following PIT. Furthermore, up-regulation of cell surface levels of CD5 may contribute to reduced reactivity to allergen.

[Association of T174M polymorphism of the angiotensinogen gene with the higher risk of cerebral stroke in women].

Science.gov (United States)

Stetskaia, T A; Bushueva, O Iu; Bulgakova, I V; Vialykh, E K; Shuteeva, T V; Biriukov, A E; Ivanov, V P; Polonikov, A V

2014-01-01

To study the association of M235T (rs699) and T174M (rs4762) polymorphisms of the angiotensinogen (AGT) gene with the risk of cerebral stroke (CS) in the Russians of the Central Chernozem Region. A total of 638 DNA samples obtained from 353 patients with CS and 285 sex- and age-matched healthy individuals were examined. The polymorphisms were genotyped by polymerase chain reaction (T174M) and TaqMan allelic discrimination (M235T) assays. Heterozygous AGT 174TM genotype carriers were found to be at a higher risk for CS (odd ratio (OR) = 1.52; 95% confidence interval (CI), 1.08-2.15; p = 0.02). A gender-stratified analysis showed that the mutant 174M allele (OR = 1.86; 95% CI, 1.14-3.03, p = 0.01) and variant 174TM and 174MM genotypes (OR = 1.86; 95% CI, 1.09-3.20; p = 0.02) were associated with the higher risk of cerebral stroke in women. The association of AGT T174M polymorphism with the risk of CS was first found; but the higher risk of the disease in the carriers of variant alleles and genotypes was observed in the women only.

Being WISE. I. Validating stellar population models and M {sub *}/L ratios at 3.4 and 4.6 μm

Energy Technology Data Exchange (ETDEWEB)

Norris, Mark A.; Meidt, Sharon; Van de Ven, Glenn; Schinnerer, Eva; Groves, Brent; Querejeta, Miguel, E-mail: norris@mpia.de [Max Planck Institut für Astronomie, Königstuhl 17, D-69117, Heidelberg (Germany)

2014-12-10

Using data from the Wide-field Infrared Survey Explorer mission, we have measured near infra-red (NIR) photometry of a diverse sample of dust-free stellar systems (globular clusters, dwarf and giant early-type galaxies) which have metallicities that span the range -2.2 < [Fe/H] (dex) < 0.3. This dramatically increases the sample size and broadens the metallicity regime over which the 3.4 (W1) and 4.6 μm (W2) photometry of stellar populations have been examined. We find that the W1 – W2 colors of intermediate and old (>2 Gyr) stellar populations are insensitive to the age of the stellar population, but that the W1 – W2 colors become bluer with increasing metallicity, a trend not well reproduced by most stellar population synthesis (SPS) models. In common with previous studies, we attribute this behavior to the increasing strength of the CO absorption feature located in the 4.6 μm bandpass with metallicity. Having used our sample to validate the efficacy of some of the SPS models, we use these models to derive stellar mass-to-light ratios in the W1 and W2 bands. Utilizing observational data from the SAURON and ATLAS3D surveys, we demonstrate that these bands provide extremely simple, yet robust stellar mass tracers for dust free older stellar populations that are freed from many of the uncertainties common among optical estimators.

Tools-4-Metatool (T4M): online suite of web-tools to process stoichiometric network analysis data from Metatool.

Science.gov (United States)

Xavier, Daniela; Vázquez, Sara; Higuera, Clara; Morán, Federico; Montero, Francisco

2011-08-01

Tools-4-Metatool (T4M) is a suite of web-tools, implemented in PERL, which analyses, parses, and manipulates files related to Metatool. Its main goal is to assist the work with Metatool. T4M has two major sets of tools: Analysis and Compare. Analysis visualizes the results of Metatool (convex basis, elementary flux modes, and enzyme subsets) and facilitates the study of metabolic networks. It is composed of five tools: MDigraph, MetaMatrix, CBGraph, EMGraph, and SortEM. Compare was developed to compare different Metatool results from different networks. This set consists of: Compara and ComparaSub which compare network subsets providing outputs in different formats and ComparaEM that seeks for identical elementary modes in two metabolic networks. The suite T4M also includes one script that generates Metatool input: CBasis2Metatool, based on a Metatool output file that is filtered by a list of convex basis' metabolites. Finally, the utility CheckMIn checks the consistency of the Metatool input file. T4M is available at http://solea.quim.ucm.es/t4m. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

AT-101 enhances gefitinib sensitivity in non-small cell lung cancer with EGFR T790M mutations

International Nuclear Information System (INIS)

Zhao, Ren; Zhou, Shun; Xia, Bing; Zhang, Cui-ying; Hai, Ping; Zhe, Hong; Wang, Yan-yang

2016-01-01

Although epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) have become the standard care of patients with advanced EGFR-mutant non-small cell lung cancer (NSCLC), development of acquired resistance is inevitable. A secondary mutation of threonine 790 (T790M) is associated with approximately half of the cases of acquired resistance. Strategies or agents to overcome this type of resistance are still limited. In this study, enhanced antitumor effect of AT-101, a-pan-Bcl-2 inhibitor, on gefitinib was explored in NSCLC with T790M mutation. The effect of cotreatment with AT-101 and gefitinib on the viability of NSCLC cell lines harboring acquired T790M mutation was investigated using the MTT assay. The cellular apoptosis of NSCLC cells after cotreatment with AT-101 and gefitinib was assessed by FITC-annexin V/PI assay and Western blots analysis. The potential underlying mechanisms of the enhanced therapeutic effect for AT-101 was also studied using Western blots analysis. The in vivo anti-cancer efficacy of the combination with AT-101 and gefitinib was examined in a mouse xenograft model. In this study, we found that treatment with AT-101 in combination with gefitinib significantly inhibited cell proliferation, as well as promoted apoptosis of EGFR TKIs resistant lung cancer cells. The apoptotic effects of the use of AT-101 was related to the blocking of antiapoptotic protein: Bcl-2, Bcl-xl, and Mcl-1 and downregrulation of the molecules in EGFR pathway. The observed enhancements of tumor growth suppression in xenografts supported the reverse effect of AT-101 in NSCLC with T790M mutation, which has been found in in vitro studies before. AT-101 enhances gefitinib sensitivity in NSCLC with EGFR T790M mutations. The addition of AT-101 to gefitinib is a promising strategy to overcome EGFR TKIs resistance in NSCLC with EGFR T790M mutations

Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques

Directory of Open Access Journals (Sweden)

Zhongjie Xu

2016-06-01

Full Text Available Di-2-pyridylketone-4,4,-dimethyl-3-thiosemicarbazone (Dp44mT exhibits significant antitumor activity. However, the mechanism of its pharmacological interaction with human serum albumin (HSA and DNA remains poorly understood. Here, we aimed to elucidate the interactions of Dp44mT with HSA and DNA using MTT assays, spectroscopic methods, and molecular docking analysis. Our results indicated that addition of HSA at a ratio of 1:1 did not alter the cytotoxicity of Dp44mT, but did affect the cytotoxicity of the Dp44mT-Cu complex. Data from fluorescence quenching and UV-VIS absorbance measurements demonstrated that Dp44mT could bind to HSA with a moderate affinity (Ka = approximately 104 M−1. CD spectra revealed that Dp44mT could slightly disrupt the secondary structure of HSA. Dp44mT could also interact with Ct-DNA, but had a moderate binding constant (KEB = approximately 104 M−1. Docking studies indicated that the IB site of HSA, but not the IIA and IIIA sites, could be favorable for Dp44mT and that binding of Dp44mT to HSA involved hydrogen bonds and hydrophobic force, consistent with thermodynamic results from spectral investigations. Thus, the moderate binding affinity of Dp44mT with HSA and DNA partially contributed to its antitumor activity and may be preferable in drug design approaches.

Crystallization and preliminary crystallographic analysis of tRNA (m7G46) methyltransferase from Escherichia coli

International Nuclear Information System (INIS)

Liu, Qi; Gao, Yang; Yang, Weili; Zhou, Huihao; Gao, Yongxiang; Zhang, Xiao; Teng, Maikun; Niu, Liwen

2008-01-01

tRNA (m 7 G46) methyltransferase from E. coli was overexpressed, purified and crystallized. Diffraction data were collected to 2.04 Å resolution. Transfer RNA (tRNA) (m 7 G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-l-methionine (SAM) as the methyl-group donor to catalyze the formation of N 7 -methylguanosine (m 7 G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His 6 tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P2 1

Vahepõlvkonna idealiseeritud mälestused / Ernest Truely ; tõlkinud Ulla Juske

Index Scriptorium Estoniae

Truely, Ernest

2011-01-01

Fotokuu näitus "Sündinud samas kohas: kujutis, mälu ja fiktsioon Baltimaades" Tallinna Kunstihoones 20. nov.-ni 2011. Kuraator Vytautas Michelkevičius (Leedu), kujundaja Dénes Farkas. Sigrid Viiri, Marge Monko (Eesti), Tadas Šarūnase (Leedu), Kaspars Podnieksi ja Arnis Balčuse (Läti) töödest

Identification of candidates for postmastectomy radiotherapy in patients with pT3N0M0 breast cancer

International Nuclear Information System (INIS)

Hamamoto, Yasushi; Ohsumi, Shozo; Aogi, Kenjiro; Takashima, Shigemitsu; Shinohara, Shuichi; Nakajima, Naomi; Kataoka, Masaaki

2013-01-01

There is still controversy concerning the indication of postmastectomy radiotherapy (PMRT) for pT3N0M0 breast cancer. To identify the candidates for PMRT in this subset, we investigated failure patterns, and searched for risk factors for isolated locoregional failure in pT3N0M0 breast cancer after mastectomy without PMRT. Among 1,176 patients who received mastectomy without PMRT for untreated unilateral breast cancer between 1990 and 2002, 64 patients (5%) had pT3N0M0 breast cancer (age 30-81 years; median 52.5 years). Isolated locoregional failure as the initial failure occurred in three patients. For all 64 patients, the 8-year failure-free survival rate, the isolated locoregional failure-free rate, and the distant failure-free rate were 76, 93, and 82%, respectively. Incidence of isolated locoregional failure as the initial failure was 18% (2/11) for patients 40 years or younger and 2% (1/53) for patients older than 40 years. The 8-year isolated locoregional failure-free rates were 73% for patients 40 years or younger and 98% for patients older than 40 years (p=0.0135). Concerning pT3N0M0 breast cancer, incidence of isolated locoregional failure was comparatively low after mastectomy without PMRT. Routine use of PMRT for all pT3N0M0 patients seemed to be unacceptable. PMRT may be useful for younger patients because of the comparatively high incidence of isolated locoregional failure. Because of the small number of cases in our series, further studies are necessary to determine the usefulness of PMRT for younger patients with pT3N0M0 breast cancer. (author)

Cancer of the urinary bladder category T2, T3, (N/sub x/M/sub o/) treated by interstitial radium implant: second report

International Nuclear Information System (INIS)

Werf-Messing, B.; Menon, R.S.; Hop, W.C.J.

1983-01-01

Three-hundred-twenty-eight patients with bladder cancer category T 2 N/sub x/M/sub o/ have been treated by 3 times 3.5 Gy external irradiation followed by a radium implant. Overall 5- and 10-year survival in the T 2 category are 56% adn 37%. In the T 3 category they are 39% adn 13%, respectively. The intercurrent death (i.e. without evidence of bladder malignancy) corrected acuarial survival percentage in the T 2 category is 75% at 5 years and 69% at 10 years. The corresponding percentages in the T 3 category are 62% and 59%. Prognosis is worsened by the following factors: more than 1 diagnostic transurethral resection, a pathological intravenous pyelography, non-papillary structure and poor degree of differentiation of the growth. Prognosis in category T 3 , as compared with category T 2 , is worse because of the prevalence of bad prognosticators in this T 3 category. Therapeutic adaptation to thesse findings might improve prognosis in the future

The human CD8β M-4 isoform dominant in effector memory T cells has distinct cytoplasmic motifs that confer unique properties.

Directory of Open Access Journals (Sweden)

Deepshi Thakral

Full Text Available The CD8 co-receptor influences T cell recognition and responses in both anti-tumor and anti-viral immunity. During evolution in the ancestor of humans and chimpanzees, the CD8B gene acquired two additional exons. As a result, in humans, there are four CD8β splice variants (M1 to M4 that differ in their cytoplasmic tails. The M-1 isoform which is the equivalent of murine CD8β, is predominantly expressed in naïve T cells, whereas, the M-4 isoform is predominantly expressed in effector memory T cells. The characteristics of the M-4 isoform conferred by its unique 36 amino acid cytoplasmic tail are not known. In this study, we identified a dihydrophobic leucine-based receptor internalization motif in the cytoplasmic tail of M-4 that regulated its cell surface expression and downregulation after activation. Further the M-4 cytoplasmic tail was able to associate with ubiquitinated targets in 293T cells and mutations in the amino acids NPW, a potential EH domain binding site, either enhanced or inhibited the interaction. In addition, the M-4 tail was itself mono-ubiquitinated on a lysine residue in both 293T cells and a human T cell line. When peripheral blood human T cells expressed CD8αβ M-4, the frequency of MIP-1β secreting cells responding to antigen presenting cells was two-fold higher as compared to CD8αβ M-1 expressing T cells. Thus, the cytoplasmic tail of the CD8β M-4 isoform has unique characteristics, which likely contributed to its selective expression and function in human effector memory T cells.

Comparison of genetic population structure of the large blue butterflies Maculinea nausithous and M. teleius

DEFF Research Database (Denmark)

Figurny-Puchalska, Edyta; Gadeberg, Rebekka M.E.; Boomsma, Jacobus Jan

2000-01-01

We investigated the genetic population structure of two rare myrmecophilous lycaenid butterflies, Maculinea nausithous and M. teleius, which often live sympatrically and have similar biology. In Europe, both species occur in highly fragmented populations and are vulnerable to local extinction. Th...

Metastability exchange optical pumping in {sup 3}He gas up to 30 mT. Efficiency measurements and evidence of laser-induced nuclear relaxation

Energy Technology Data Exchange (ETDEWEB)

Batz, Marion

2011-07-08

Advances in metastability exchange optical pumping (MEOP) of {sup 3}He at high laser powers, with its various applications, but also at high gas pressures p{sub 3} and high magnetic field strengths B, have provided strong motivation for revisiting the understanding and for investigating the limitations of this powerful technique. For this purpose, we present systematic experimental and theoretical studies of efficiency and of relaxation mechanisms in B{<=}30 mT and p{sub 3}=0.63-2.45 mbar. {sup 3}He nuclear polarisation is measured by light absorption in longitudinal configuration where weak light beams at 1083 nm parallel to magnetic field and cell axis with opposite circular polarisations are used to probe the distribution of populations in the metastable state. This method is systematically tested to evaluate potential systematic biases and is shown to be reliable for the study of OP dynamics despite the redistribution of populations by OP light. Nuclear polarisation loss associated to the emission of polarised light by the plasma discharge used for MEOP is found to decrease above 10 mT, as expected, due to hyperfine decoupling in highly excited states. However, this does not lead to improved MEOP efficiency at high laser power. We find clear evidence of additional laser-induced relaxation instead. The strong OP-enhanced polarisation losses, currently limiting MEOP performances, are quantitatively investigated using an angular momentum budget approach and a recently developed comprehensive model that describes the combined effects of OP, ME and relaxation, validated by comparison to experimental results.

A javítási műveletek jelöltségi hipotézise

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Zsuzsanna Németh

2014-12-01

Full Text Available The markedness hypothesis of repair operations In this paper, using conversation analysis, I propose a model describing the technology of four repair operations, namely, recycling, replacement, insertion, and aborting relative to each other on the basis that they inherently halt the progressivity of the current turn-at-talk. As violating the preference for progressivity is a marked usage, interpreting the property of halting progressivity as a system of binary features in the case of the four repair operations enables us to apply the theory of markedness to describe the technology of the repair operations relative to each other. Testing the model has been possible only in an indirect way by setting up a sub-hypothesis. I have argued that not only their functions but also their degree of markedness plays a role in the selection of repair operations employed in carrying out the same action. I have tested the sub-hypothesis on a Hungarian corpus. The results support the hypothesis. A tanulmány a konverzációelemzés elméleti keretében négy javítási művelet, az ismétlés, a beszúrás, a csere és a törlés technológiáját hasonlítja össze kísérletet téve egy olyan modell létrehozására, amely a javítási műveletek jelöltségét feltételezi. Ennek alapja, hogy a spontán társalgások során alkalmazott javítási jelenségek felfüggesztik a folyamatban levő forduló, és ezáltal a folyamatban levő cselekvés előrehaladását. Mivel a progresszivitás fenntartása általános preferencia a társalgásban, megszakítása jelölt nyelvhasználatot eredményez, amelyet a négy művelet esetében három bináris jegy rendszereként értelmezhetünk (retrospektivitás, redundancia, nem megfelelő szegmentum. A modell tesztelése egy magyar nyelvű korpuszon történt egy szub-hipotézis segítségével, amelynek lényege, hogy az egyazon cselekvés során alkalmazott javítási műveletek kiválasztásában funkcióik mellett

INDOOR AND OUTDOOR SOURCE CONTRIBUTIONS TO PERSONAL PM2.5 FOR A PANEL OF INDIVIDUALS WITH CARDIOVASCULAR DISEASE OR COPD LIVING IN BOSTON, MA

Science.gov (United States)

Repeated personal, home indoor, home outdoor, and ambient particulate and gaseous pollutant levels were characterized for individuals with cardiovascular disease or COPD and their partners living in the Boston area. Health status was determined by self-reported history of myoc...

Association between angiotensinogen T174M polymorphism and ischemic stroke: A meta-analysis

Directory of Open Access Journals (Sweden)

Zilin Ou

2015-01-01

Full Text Available Background: Numerous studies have evaluated the association between the angiotensinogen (AGT T174M polymorphism and ischemic stroke(IS risk. However, the specific association is still controversial. Materials and Methods: In order to explore this association more deeply, we performed a meta-analysis. All of the relevant studies were identified from PubMed, Embase, and Chinese National Knowledge Infrastructure database up to October 2014. Statistical analyses were conducted with STATA 12.0 software. Odds ratio (OR with 95% confidence interval (CI values were applied to evaluate the strength of the association. Results: Six studies with 1290 cases and 1125 controls were included. No significant variation in IS risk was detected in any of the genetic models in the overall (MM vs. TT: OR = 1.64, 95% CI = 0.51-5.28; MT vs. TT: OR = 0.93, 95% CI = 0.66-1.31; dominant model: OR = 1.08, 95% CI = 0.69-1.72; recessive model: OR = 0.61,95% CI = 0.20-1.91. Taking into account the effect of ethnicity, further stratified analyses were performed. The results showed that AGT gene T174M polymorphism might be associated with IS risk in Asians (MM vs. TT: OR = 3.28, 95% CI = 1.79-6.02; recessive model: OR = 0.31, 95% CI = 0.17-0.57. Conclusion: In conclusion, the AGT T174M polymorphism may be a susceptible predictor of the risk of IS in Asians. Further, large and well-designed studies are needed to confirm this conclusion.

NEW M, L, AND T DWARF COMPANIONS TO NEARBY STARS FROM THE WIDE-FIELD INFRARED SURVEY EXPLORER

Energy Technology Data Exchange (ETDEWEB)

Luhman, Kevin L.; Loutrel, Nicholas P.; McCurdy, Nicholas S.; Melso, Nicole D.; Star, Kimberly M.; Terrien, Ryan C. [Department of Astronomy and Astrophysics, Pennsylvania State University, University Park, PA 16802 (United States); Mace, Gregory N.; McLean, Ian S. [UCLA Division of Astronomy and Astrophysics, Los Angeles, CA 90095 (United States); Young, Michael D.; Rhode, Katherine L. [Department of Astronomy, Indiana University, Swain West 319, 727 East Third Street, Bloomington, IN 47405 (United States); Davy Kirkpatrick, J., E-mail: kluhman@astro.psu.edu [Infrared Processing and Analysis Center, MS 100-22, California Institute of Technology, Pasadena, CA 91125 (United States)

2012-12-01

We present 11 candidate late-type companions to nearby stars identified with data from the Wide-field Infrared Survey Explorer (WISE) and the Two Micron All Sky Survey (2MASS). Eight of the candidates are likely to be companions based on their common proper motions with the primaries. The remaining three objects are rejected as companions, one of which is a free-floating T7 dwarf. Spectral types are available for five of the companions, which consist of M2V, M8.5V, L5, T8, and T8. Based on their photometry, the unclassified companions are probably two mid-M dwarfs and one late-M/early-L dwarf. One of the T8 companions, WISE J142320.84+011638.0, has already been reported by Pinfield and coworkers. The other T8 companion, ULAS J095047.28+011734.3, was discovered by Burningham and coworkers through the United Kingdom Infrared Telescope Infrared Deep Sky Survey, but its companionship has not been previously recognized in the literature. The L5 companion, 2MASS J17430860+8526594, is a new member of a class of L dwarfs that exhibit unusually blue near-IR colors. Among the possible mechanisms that have been previously proposed for the peculiar colors of these L dwarfs, low metallicity does not appear to be a viable explanation for 2MASS J17430860+8526594 since our spectrum of the primary suggests that its metallicity is not significantly subsolar.

NEW M, L, AND T DWARF COMPANIONS TO NEARBY STARS FROM THE WIDE-FIELD INFRARED SURVEY EXPLORER

International Nuclear Information System (INIS)

Luhman, Kevin L.; Loutrel, Nicholas P.; McCurdy, Nicholas S.; Melso, Nicole D.; Star, Kimberly M.; Terrien, Ryan C.; Mace, Gregory N.; McLean, Ian S.; Young, Michael D.; Rhode, Katherine L.; Davy Kirkpatrick, J.

2012-01-01

We present 11 candidate late-type companions to nearby stars identified with data from the Wide-field Infrared Survey Explorer (WISE) and the Two Micron All Sky Survey (2MASS). Eight of the candidates are likely to be companions based on their common proper motions with the primaries. The remaining three objects are rejected as companions, one of which is a free-floating T7 dwarf. Spectral types are available for five of the companions, which consist of M2V, M8.5V, L5, T8, and T8. Based on their photometry, the unclassified companions are probably two mid-M dwarfs and one late-M/early-L dwarf. One of the T8 companions, WISE J142320.84+011638.0, has already been reported by Pinfield and coworkers. The other T8 companion, ULAS J095047.28+011734.3, was discovered by Burningham and coworkers through the United Kingdom Infrared Telescope Infrared Deep Sky Survey, but its companionship has not been previously recognized in the literature. The L5 companion, 2MASS J17430860+8526594, is a new member of a class of L dwarfs that exhibit unusually blue near-IR colors. Among the possible mechanisms that have been previously proposed for the peculiar colors of these L dwarfs, low metallicity does not appear to be a viable explanation for 2MASS J17430860+8526594 since our spectrum of the primary suggests that its metallicity is not significantly subsolar.

Detection of T. vaginalis,M. hominis,M. genitalium, C. trachomatis, N. gonorrhoeae and U. urealyticum using Multiplex PCR

Directory of Open Access Journals (Sweden)

Tamara Brunelli

2013-03-01

Full Text Available Intoduction. The sexually transmitted diseases include a large group of infections affecting both the sexes. In this study we evaluated the prevalence of Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae and Ureaplasma urealyticum in the Prato area during the period September 2010 – July 2011. Methods.We analysed different kind of samples (urine, endocervical swabs, urethral swabs, seminal fluids from hospitalized patients or referred to the Prato clinic subjects.The DNA was obtained using EZ1-DNA extraction kit and EZ1 instrument.The DNA was then amplified using the Seeplex STD6 kit (Seegene, Korea, identifying multiple pathogens simultaneously (T. vaginalis, M. hominis, M. genitalium, C. trachomatis, N. gonorrhoeae e U. urealyticum. The revelation was performed by electrophoresis on microchip (instrument Multina, Shimadzu, Japan. Results. 1136 samples from Italian and foreign patients were examined: 876 were endocervical swabs (77%, 103 urethral swabs (9%, 103 seminal fluids (9%, and 54 urines (5%. The number of females was higher than males [894 (78.7% vs 242 (21.3%]; the mean age of females was 37.0±11.6 years, whereas that of males was 41.5 ±12.63 years.The prevalence of urogenital pathogens was: 15 positive samples for T. vaginalis (1.3%, 56 for M. hominis (4.9%, 13 for M. genitalium (1.1%, 28 for C. trachomatis (2.5%, 8 for N. gonorrhoeae (0.7% and 87 for U. urealyticum (7.7%.Among all positive, 25 subjects were positive for more than one pathogen and in particular: one was positive for the presence of 4 pathogens, five presented 3 pathogens simultaneously and the remaining nineteen for 2 pathogens. Conclusions. This study provides data on the prevalence of sexually transmitted diseases in the hospital of Prato.

Pre-existing malignancy results in increased prevalence of distinct populations of CD4+ T cells during sepsis.

Science.gov (United States)

Xie, Jianfeng; Robertson, Jennifer M; Chen, Ching-Wen; Zhang, Wenxiao; Coopersmith, Craig M; Ford, Mandy L

2018-01-01

The presence of pre-existing malignancy in murine hosts results in increased immune dysregulation and risk of mortality following a septic insult. Based on the known systemic immunologic changes that occur in cancer hosts, we hypothesized that the presence of pre-existing malignancy would result in phenotypic and functional changes in CD4+ T cell responses following sepsis. In order to conduct a non-biased, unsupervised analysis of phenotypic differences between CD4+ T cell compartments, cohorts of mice were injected with LLC1 tumor cells and tumors were allowed to grow for 3 weeks. These cancer hosts and age-matched non-cancer controls were then subjected to CLP. Splenocytes were harvested at 24h post CLP and flow cytometry and SPADE (Spanning-tree Progression Analysis of Density-normalized Events) were used to analyze populations of CD4+ cells most different between the two groups. Results indicated that relative to non-cancer controls, cancer mice contained more resting memory CD4+ T cells, more activated CD4+ effectors, and fewer naïve CD4+ T cells during sepsis, suggesting that the CD4+ T cell compartment in cancer septic hosts is one of increased activation and differentiation. Moreover, cancer septic animals exhibited expansion of two distinct subsets of CD4+ T cells relative to previously healthy septic controls. Specifically, we identified increases in both a PD-1hi population and a distinct 2B4hi BTLAhi LAG-3hi population in cancer septic animals. By combining phenotypic analysis of exhaustion markers with functional analysis of cytokine production, we found that PD-1+ CD4+ cells in cancer hosts failed to make any cytokines following CLP, while the 2B4+ PD-1lo cells in cancer mice secreted increased TNF during sepsis. In sum, the immunophenotypic landscape of cancer septic animals is characterized by both increased CD4+ T cell activation and exhaustion, findings that may underlie the observed increased mortality in mice with pre-existing malignancy

Pre-existing malignancy results in increased prevalence of distinct populations of CD4+ T cells during sepsis.

Directory of Open Access Journals (Sweden)

Jianfeng Xie

Full Text Available The presence of pre-existing malignancy in murine hosts results in increased immune dysregulation and risk of mortality following a septic insult. Based on the known systemic immunologic changes that occur in cancer hosts, we hypothesized that the presence of pre-existing malignancy would result in phenotypic and functional changes in CD4+ T cell responses following sepsis. In order to conduct a non-biased, unsupervised analysis of phenotypic differences between CD4+ T cell compartments, cohorts of mice were injected with LLC1 tumor cells and tumors were allowed to grow for 3 weeks. These cancer hosts and age-matched non-cancer controls were then subjected to CLP. Splenocytes were harvested at 24h post CLP and flow cytometry and SPADE (Spanning-tree Progression Analysis of Density-normalized Events were used to analyze populations of CD4+ cells most different between the two groups. Results indicated that relative to non-cancer controls, cancer mice contained more resting memory CD4+ T cells, more activated CD4+ effectors, and fewer naïve CD4+ T cells during sepsis, suggesting that the CD4+ T cell compartment in cancer septic hosts is one of increased activation and differentiation. Moreover, cancer septic animals exhibited expansion of two distinct subsets of CD4+ T cells relative to previously healthy septic controls. Specifically, we identified increases in both a PD-1hi population and a distinct 2B4hi BTLAhi LAG-3hi population in cancer septic animals. By combining phenotypic analysis of exhaustion markers with functional analysis of cytokine production, we found that PD-1+ CD4+ cells in cancer hosts failed to make any cytokines following CLP, while the 2B4+ PD-1lo cells in cancer mice secreted increased TNF during sepsis. In sum, the immunophenotypic landscape of cancer septic animals is characterized by both increased CD4+ T cell activation and exhaustion, findings that may underlie the observed increased mortality in mice with pre

Draft genome sequence of Serratia sp. strain M24T3, isolated from pinewood disease nematode Bursaphelenchus xylophilus.

Science.gov (United States)

Proença, Diogo Neves; Espírito Santo, Christophe; Grass, Gregor; Morais, Paula V

2012-07-01

Here we report the draft genome sequence of Serratia sp. strain M24T3, which is associated with pinewood nematode Bursaphelenchus xylophilus, the causative agent of pine wilt disease. Serratia sp. strain M24T3 has been identified as a bionematocide for B. xylophilus in vitro, and multiple genes potentially involved in virulence and nematotoxity were identified.

Draft Genome Sequence of Serratia sp. Strain M24T3, Isolated from Pinewood Disease Nematode Bursaphelenchus xylophilus

OpenAIRE

Proença, Diogo Neves; Espírito Santo, Christophe; Grass, Gregor; Morais, Paula V.

2012-01-01

Here we report the draft genome sequence of Serratia sp. strain M24T3, which is associated with pinewood nematode Bursaphelenchus xylophilus, the causative agent of pine wilt disease. Serratia sp. strain M24T3 has been identified as a bionematocide for B. xylophilus in vitro, and multiple genes potentially involved in virulence and nematotoxity were identified.

Reklāmas ietekme uz uzņēmuma tēla veidošanos integrētā mārketinga komunikācijās. Olimpiskā centra "Ventspils" piemērs

OpenAIRE

Boitmane, Līva

2008-01-01

Latvijā pēdējo gadu laikā tiek atvērti arvien jauni Olimpiskie centri, tādēļ bakalaura darba „Reklāmas ietekme uz uzņēmuma tēlu integrētā mārketinga komunikācijās. Olimpiskā centra „Ventspils” piemērs”, mērķis ir izpētīt, kā Olimpiskais centrs „Ventspils” veido savu uzņēmuma tēlu, vai strādājot pie pakalpojumu reklāmas izveides tiek mērķtiecīgi veidots Olimpiskā centra tēls jeb imidžs. Darbā sākotnēji tiek analizēta integrētā mārketinga komunikācijas teorija, aprakstīta viena no tā sastāv...

Homeostasis of peripheral CD4+ T cells: IL-2R alpha and IL-2 shape a population of regulatory cells that controls CD4+ T cell numbers

NARCIS (Netherlands)

Almeida, Afonso R. M.; Legrand, Nicolas; Papiernik, Martine; Freitas, António A.

2002-01-01

We show that the lymphoid hyperplasia observed in IL-2Ralpha- and IL-2-deficient mice is due to the lack of a population of regulatory cells essential for CD4 T cell homeostasis. In chimeras reconstituted with bone marrow cells from IL-2Ralpha-deficient donors, restitution of a population of

Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers

DEFF Research Database (Denmark)

Tomaszewski, Céline; Byrne, Stephen; Foito, Alexandra

2012-01-01

Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, significant efforts have been made to develop molecular marker strategies to allow cost-effective characterization of a large number of loci simultaneously. One such strategy involves using DAr......T markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the first use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used...

Association between the interleukin-1β C-511T polymorphism and periodontitis: a meta-analysis in the Chinese population.

Science.gov (United States)

Wang, H F; He, F Q; Xu, C J; Li, D M; Sun, X J; Chi, Y T; Guo, W

2017-02-23

The association between the interleukin-1 beta (IL-1β) C-511T (or rs16944) polymorphism and periodontitis remains inconclusive, even though there have been previous studies on this association. To assess the effects of IL-1β C-511T variants on the risk of development of periodontitis, a meta-analysis was performed in a single ethnic population. Studies, published up to December 2015, were selected for the meta-analysis from PubMed and Chinese databases. The associations were assessed with pooled OR and 95%CI. This meta-analysis identified 8 studies, including 1276 periodontitis cases and 1558 controls. Overall, a significant association between the IL-1β C-511T polymorphism and periodontitis was found in the Chinese population (TT vs CC: OR = 1.48, 95%CI = 1.19-1.85; TT + CT vs CC: OR = 1.50, 95%CI = 1.25-1.81; T vs C: OR = 1.33, 95%CI = 1.06-1.68). In the subgroup analyses based on geographical area(s), source of controls, and type of periodontitis, significant results were obtained for the association between IL-1β C-511T variants and periodontitis. Our meta-analysis indicated that the IL-1β C-511T polymorphism may be a genetic susceptibility factor for periodontitis in the Chinese population. This marker could be used to identify Chinese individuals at a high risk for periodontitis.

FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

DEFF Research Database (Denmark)

Ryder, L Rebekka; Bartels, Else Marie; Woetmann, Anders

2012-01-01

Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired...... samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero......-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from...

Aging-dependent DNA hypermethylation and gene expression of GSTM1 involved in T cell differentiation.

Science.gov (United States)

Yeh, Shu-Hui; Liu, Cheng-Ling; Chang, Ren-Chieh; Wu, Chih-Chiang; Lin, Chia-Hsueh; Yang, Kuender D

2017-07-25

This study investigated whether aging was associated with epigenetic changes of DNA hypermethylation on immune gene expression and lymphocyte differentiation. We screened CG sites of methylation in blood leukocytes from different age populations, picked up genes with age-related increase of CG methylation content more than 15%, and validated immune related genes with CG hypermethylation involved in lymphocyte differentiation in the aged population. We found that 12 genes (EXHX1、 IL-10、 TSP50、 GSTM1、SLC5A5、SPI1、F2R、LMO2、PTPN6、FGFR2、MMP9、MET) were associated with promoter or exon one DNA hypermethylation in the aged group. Two immune related genes, GSTM1 and LMO2, were chosen to validate its aging-related CG hypermethylation in different leukocytes. We are the first to validate that GSTM1_P266 and LMO2_E128 CG methylation contents in T lymphocytes but not polymorphonuclear cells (PMNs) or mononuclear cells (MNCs) were significantly increased in the aged population. The GSTM1 mRNA expression in T lymphocytes but not PMNs or MNCs was inversely associated with the GSTM1 CG hypermethylation levels in the aged population studied. Further studies showed that lower GSTM1 CG methylation content led to the higher GSTM1 mRNA expression in T cells and knockdown of GSTM1 mRNA expression decreased type 1 T helper cell (Th1) differentiation in Jurkat T cells and normal adult CD4 T cells. The GSTM1_P266 hypermethylation in the aged population associated with lower GSTM1 mRNA expression was involved in Th1 differentiation, highlighting that modulation of aging-associated GSTM1 methylation may be able to enhance T helper cell immunity in the elders.

Surface Gravities for 228 M, L, and T Dwarfs in the NIRSPEC Brown Dwarf Spectroscopic Survey

International Nuclear Information System (INIS)

Martin, Emily C.; Mace, Gregory N.; McLean, Ian S.; Logsdon, Sarah E.; Rice, Emily L.; Kirkpatrick, J. Davy; Burgasser, Adam J.; McGovern, Mark R.; Prato, Lisa

2017-01-01

We combine 131 new medium-resolution ( R ∼ 2000) J -band spectra of M, L, and T dwarfs from the Keck NIRSPEC Brown Dwarf Spectroscopic Survey (BDSS) with 97 previously published BDSS spectra to study surface-gravity-sensitive indices for 228 low-mass stars and brown dwarfs spanning spectral types M5–T9. Specifically, we use an established set of spectral indices to determine surface gravity classifications for all of the M6–L7 objects in our sample by measuring the equivalent widths (EW) of the K i lines at 1.1692, 1.1778, and 1.2529 μ m, and the 1.2 μ m FeH J absorption index. Our results are consistent with previous surface gravity measurements, showing a distinct double peak—at ∼L5 and T5—in K i EW as a function of spectral type. We analyze the K i EWs of 73 objects of known ages and find a linear trend between log(Age) and EW. From this relationship, we assign age ranges to the very low gravity, intermediate gravity, and field gravity designations for spectral types M6–L0. Interestingly, the ages probed by these designations remain broad, change with spectral type, and depend on the gravity-sensitive index used. Gravity designations are useful indicators of the possibility of youth, but current data sets cannot be used to provide a precise age estimate.

Surface Gravities for 228 M, L, and T Dwarfs in the NIRSPEC Brown Dwarf Spectroscopic Survey

Energy Technology Data Exchange (ETDEWEB)

Martin, Emily C.; Mace, Gregory N.; McLean, Ian S.; Logsdon, Sarah E. [Department of Physics and Astronomy, University of California Los Angeles, 430 Portola Plaza, Box 951547, Los Angeles, CA 90095-1547 (United States); Rice, Emily L. [Department of Engineering Science and Physics, College of Staten Island, 2800 Victory Boulevard, Staten Island, NY 10301 (United States); Kirkpatrick, J. Davy [Infrared Processing and Analysis Center, MS 100-22, California Institute of Technology, Pasadena, CA 91125 (United States); Burgasser, Adam J. [Center for Astrophysics and Space Science, University of California San Diego, La Jolla, CA 92093 (United States); McGovern, Mark R. [Math and Sciences Division, Antelope Valley College, 3041 West Avenue K, Lancaster, CA 93536 (United States); Prato, Lisa, E-mail: emartin@astro.ucla.edu [Lowell Observatory, 1400 West Mars Hill Road, Flagstaff, AZ 86001 (United States)

2017-03-20

We combine 131 new medium-resolution ( R ∼ 2000) J -band spectra of M, L, and T dwarfs from the Keck NIRSPEC Brown Dwarf Spectroscopic Survey (BDSS) with 97 previously published BDSS spectra to study surface-gravity-sensitive indices for 228 low-mass stars and brown dwarfs spanning spectral types M5–T9. Specifically, we use an established set of spectral indices to determine surface gravity classifications for all of the M6–L7 objects in our sample by measuring the equivalent widths (EW) of the K i lines at 1.1692, 1.1778, and 1.2529 μ m, and the 1.2 μ m FeH{sub J} absorption index. Our results are consistent with previous surface gravity measurements, showing a distinct double peak—at ∼L5 and T5—in K i EW as a function of spectral type. We analyze the K i EWs of 73 objects of known ages and find a linear trend between log(Age) and EW. From this relationship, we assign age ranges to the very low gravity, intermediate gravity, and field gravity designations for spectral types M6–L0. Interestingly, the ages probed by these designations remain broad, change with spectral type, and depend on the gravity-sensitive index used. Gravity designations are useful indicators of the possibility of youth, but current data sets cannot be used to provide a precise age estimate.

Measuring hepatic functional reserve using T1 mapping of Gd-EOB-DTPA enhanced 3T MR imaging: A preliminary study comparing with 99mTc GSA scintigraphy and signal intensity based parameters.

Science.gov (United States)

Nakagawa, Masataka; Namimoto, Tomohiro; Shimizu, Kie; Morita, Kosuke; Sakamoto, Fumi; Oda, Seitaro; Nakaura, Takeshi; Utsunomiya, Daisuke; Shiraishi, Shinya; Yamashita, Yasuyuki

2017-07-01

To determine the utility of liver T1-mapping on gadolinium-ethoxybenzyl-diethylenetriamine-pentaacetic acid (Gd-EOB-DTPA) enhanced magnetic resonance (MR) imaging for the measurement of liver functional reserve compared with the signal intensity (SI) based parameters, technetium-99m-galactosyl serum albumin ( 99m Tc-GSA) scintigraphy and indocyanine green (ICG) clearance. This retrospective study included 111 patients (Child-Pugh-A 90; -B 21) performed with both Gd-EOB-DTPA enhanced liver MR imaging and 99m Tc-GSA (76 patients with ICG). Receiver operating characteristic (ROC) curve analysis was performed to compare diagnostic performances of T1-relaxation-time parameters [pre-(T1pre) and post-contrast (T1hb) Gd-EOB-DTPA], SI based parameters [relative enhancement (RE), liver-to-muscle-ratio (LMR), liver-to-spleen-ratio (LSR)] and 99m Tc-GSA scintigraphy blood clearance index (HH15)] for Child-Pugh classification. Pearson's correlation was used for comparisons among T1-relaxation-time parameters, SI-based parameters, HH15 and ICG. A significant difference was obtained for Child-Pugh classification with T1hb, ΔT1, all SI based parameters and HH15. T1hb had the highest AUC followed by RE, LMR, LSR, ΔT1, HH15 and T1pre. The correlation coefficients with HH15 were T1pre 0.22, T1hb 0.53, ΔT1 -0.38 of T1 relaxation parameters; RE -0.44, LMR -0.45, LSR -0.43 of SI-based parameters. T1hb was highest for correlation with HH15. The correlation coefficients with ICG were T1pre 0.29, T1hb 0.64, ΔT1 -0.42 of T1 relaxation parameters; RE -0.50, LMR -0.61, LSR -0.58 of SI-based parameters; 0.64 of HH15. Both T1hb and HH15 were highest for correlation with ICG. T1 relaxation time at post-contrast of Gd-EOB-DTPA (T1hb) was strongly correlated with ICG clearance and moderately correlated HH15 with 99m Tc-GSA. T1hb has the potential to provide robust parameter of liver functional reserve. Copyright © 2017 Elsevier B.V. All rights reserved.

Methylenetetrahydrofolate reductase C677T polymorphism in patients with lung cancer in a Korean population

Directory of Open Access Journals (Sweden)

Yun Woo-Jun

2011-02-01

Full Text Available Abstract Background This study was designed to investigate an association between methylenetetrahydrofolate reductase (MTHFR C677T polymorphism and the risk of lung cancer in a Korean population. Methods We conducted a large-scale, case-control study involving 3938 patients with newly diagnosed lung cancer and 1700 healthy controls. Genotyping was performed with peripheral blood DNA for MTHFR C677T polymorphisms. Statistical significance was estimated by logistic regression analysis. Results The MTHFR C677T frequencies of CC, CT, and TT genotypes were 34.5%, 48.5%, and 17% among lung cancer patients, and 31.8%, 50.7%, and 17.5% in the controls, respectively. The MTHFR 677CT and TT genotype showed a weak protection against lung cancer compared with the homozygous CC genotype, although the results did not reach statistical significance. The age- and gender-adjusted odds ratio (OR of overall lung cancer was 0.90 (95% confidence interval (CI, 0.77-1.04 for MTHFR 677 CT and 0.88 (95% CI, 0.71-1.07 for MTHFR 677TT. However, after stratification analysis by histological type, the MTHFR 677CT genotype showed a significantly decreased risk for squamous cell carcinoma (age- and gender-adjusted OR, 0.78; 95% CI, 0.64-0.96. The combination of 677 TT homozygous with 677 CT heterozygous also appeared to have a protection effect on the risk of squamous cell carcinoma. We observed no significant interaction between the MTHFR C677T polymorphism and age and gender or smoking habit. Conclusions This is the first reported study focusing on the association between MTHFR C677T polymorphisms and the risk of lung cancer in a Korean population. The T allele was found to provide a weak protective association with lung squamous cell carcinoma.

The earliest settlers' antiquity and evolutionary history of Indian populations: evidence from M2 mtDNA lineage

Directory of Open Access Journals (Sweden)

Kotal M

2008-08-01

Full Text Available Abstract Background The "out of Africa" model postulating single "southern route" dispersal posits arrival of "Anatomically Modern Human" to Indian subcontinent around 66–70 thousand years before present (kyBP. However the contributions and legacy of these earliest settlers in contemporary Indian populations, owing to the complex past population dynamics and later migrations has been an issue of controversy. The high frequency of mitochondrial lineage "M2" consistent with its greater age and distribution suggests that it may represent the phylogenetic signature of earliest settlers. Accordingly, we attempted to re-evaluate the impact and contribution of earliest settlers in shaping the genetic diversity and structure of contemporary Indian populations; using our newly sequenced 72 and 4 published complete mitochondrial genomes of this lineage. Results The M2 lineage, harbouring two deep rooting subclades M2a and M2b encompasses approximately one tenth of the mtDNA pool of studied tribes. The phylogeographic spread and diversity indices of M2 and its subclades among the tribes of different geographic regions and linguistic phyla were investigated in detail. Further the reconstructed demographic history of M2 lineage as a surrogate of earliest settlers' component revealed that the demographic events with pronounced regional variations had played pivotal role in shaping the complex net of populations phylogenetic relationship in Indian subcontinent. Conclusion Our results suggest that tribes of southern and eastern region along with Dravidian and Austro-Asiatic speakers of central India are the modern representatives of earliest settlers of subcontinent. The Last Glacial Maximum aridity and post LGM population growth mechanised some sort of homogeneity and redistribution of earliest settlers' component in India. The demic diffusion of agriculture and associated technologies around 3 kyBP, which might have marginalized hunter-gatherer, is

Basketbola kluba "Ventspils" mārketinga komunikācija sociālajos tīklos "Instagram" un "Facebook" 2016.-2017. gada sezonā

OpenAIRE

Purnis, Roberts

2017-01-01

Bakalaura darba nosaukums ir “Basketbola kluba “Ventspils” mārketinga komunikācija sociālajos tīklos "Instagram" un "Facebook" 2016.-2017. gada sezonā.” Pētījuma mērķis ir noskaidrot, kā sociālajos tīklos Facebook un Instagram tiek komunicēts ar mērķa publikām, kā iesaistīta auditorija- skatītāji, fani, abonementu īpašnieki. Darba teorētisko pamatu veido informācija par sporta, sociālo tīklu mārketingu, kā arī sociālo tīklu un reklāmas teorija. Darbā analizēti basketbola kluba “Ventspils” ier...

ORIGIN OF 12 μm EMISSION ACROSS GALAXY POPULATIONS FROM WISE AND SDSS SURVEYS

International Nuclear Information System (INIS)

Donoso, E.; Yan Lin; Tsai, C.; Eisenhardt, P.; Stern, D.; Assef, R. J.; Leisawitz, D.; Jarrett, T. H.; Stanford, S. A.

2012-01-01

We cross-matched Wide-field Infrared Survey Explorer sources brighter than 1 mJy at 12 μm with the Sloan Digital Sky Survey galaxy spectroscopic catalog to produce a sample of ∼10 5 galaxies at (z) = 0.08, the largest of its kind. This sample is dominated (70%) by star-forming (SF) galaxies from the blue sequence, with total IR luminosities in the range ∼10 8 -10 12 L ☉ . We identify which stellar populations are responsible for most of the 12 μm emission. We find that most (∼80%) of the 12 μm emission in SF galaxies is produced by stellar populations younger than 0.6 Gyr. In contrast, the 12 μm emission in weak active galactic nuclei (AGNs; L [Oiii] 7 L . ) is produced by older stars, with ages of ∼1-3 Gyr. We find that L 12μm linearly correlates with stellar mass for SF galaxies. At fixed 12 μm luminosity, weak AGNs deviate toward higher masses since they tend to be hosted by massive, early-type galaxies with older stellar populations. SF galaxies and weak AGNs follow different L 12μm -SFR (star formation rate) relations, with weak AGNs showing excess 12 μm emission at low SFR (0.02-1 M ☉ yr –1 ). This is likely due to dust grains heated by older stars. While the specific star formation rate (SSFR) of SF galaxies is nearly constant, the SSFR of weak AGNs decreases by ∼3 orders of magnitude, reflecting the very different star formation efficiencies between SF galaxies and massive, early-type galaxies. Stronger type II AGNs in our sample (L[ Oiii] > 10 7 L . ), act as an extension of massive SF galaxies, connecting the SF and weak AGN sequences. This suggests a picture where galaxies form stars normally until an AGN (possibly after a starburst episode) starts to gradually quench the SF activity. We also find that 4.6-12 μm color is a useful first-order indicator of SF activity in a galaxy when no other data are available.

Montovaná konstrukce haly ve Vysokém Mýtě

OpenAIRE

Jarmara, Pavel

2017-01-01

Tato diplomová práce se zabývá návrhem a statickým výpočtem montované, skladovací haly. V rámci práce jsem navrhnul a posoudil předpjatý vazník, železobetonový vazník, vazničku, sloup a kalichovou patku. Prvky jsou posouzeny na mezní stav únosnosti. Řešení vnitřních sil vazníků bylo provedeno ručním výpočtem. Vnitřní síly sloupu a patky byly určeny v programu Scia Engenner. Uvedené výpočty jsou provedeny dle Eurokódu 2. Ke všem navrženým prvků jsem také narýsoval výkresy tvaru a výztuže. T...

Effect of 0.4 mT power frequency magnetic field on F-actin assembly of CHL cells

International Nuclear Information System (INIS)

Chu Keping; Cai Zhiyin; Zhang Yukun; Xia Nuohong

2007-01-01

Objective: To investigate the effect of 0.4mT power frequency magnetic field on the microfilament (F- actin) assembly of Chinese hamster lung (CHL) cells. Methods: F-actin were marked with immunohistochemical method, then observed under a confocal microscope. The content of ECFRs in the preparation of the detergent-insoluble cytoskeleton was measured with Western-blotting. Results: The stress fiber's of CHL cells decreased after exposure to 0.4mT power frequency magnetic field for 30min, as well as after treatment with epidermal growth factor (ECF) of 50nM. Filopodias appeared at the periphery after exposure to magnetic field as well as treatment with EGF. The EGF receptor mass associated with the detergent-insoluble cytoskeleton increased after exposure to magnetic field as well as treatment with EGF. Conclusion: 0.4mT power frequency magnetic field induced assembly of F-actin in CHL cells. The change induced by magnetic field would be related to clustering of EGFR induced by magnetic field and passing the signal down. (authors)

Genetic variation, population structure and linkage disequilibrium in Switchgrass with ISSR, SCoT and EST-SSR markers.

Science.gov (United States)

Zhang, Yu; Yan, Haidong; Jiang, Xiaomei; Wang, Xiaoli; Huang, Linkai; Xu, Bin; Zhang, Xinquan; Zhang, Lexin

2016-01-01

To evaluate genetic variation, population structure, and the extent of linkage disequilibrium (LD), 134 switchgrass ( Panicum virgatum L.) samples were analyzed with 51 markers, including 16 ISSRs, 20 SCoTs, and 15 EST-SSRs. In this study, a high level of genetic variation was observed in the switchgrass samples and they had an average Nei's gene diversity index (H) of 0.311. A total of 793 bands were obtained, of which 708 (89.28 %) were polymorphic. Using a parameter marker index (MI), the efficiency of the three types of markers (ISSR, SCoT, and EST-SSR) in the study were compared and we found that SCoT had a higher marker efficiency than the other two markers. The 134 switchgrass samples could be divided into two sub-populations based on STRUCTURE, UPGMA clustering, and principal coordinate analyses (PCA), and upland and lowland ecotypes could be separated by UPGMA clustering and PCA analyses. Linkage disequilibrium analysis revealed an average r 2 of 0.035 across all 51 markers, indicating a trend of higher LD in sub-population 2 than that in sub-population 1 ( P  < 0.01). The population structure revealed in this study will guide the design of future association studies using these switchgrass samples.

Android Kötücül Yazılım Tespit Sistemleri İncelemesi

Directory of Open Access Journals (Sweden)

Ömer KİRAZ

2017-10-01

Full Text Available Akıllı telefonların hayatımıza girmesiyle birlikte akıllı telefonları kullanan kullanıcı sayısı her geçen gün artarak devam etmektedir. Akıllı telefonların fazla talep görmesindeki neden, insanların bir cihazla istedikleri işleri tek dokunuşla kolaylıkla yapabilmesidir. International Data Corporation (IDC firmasının 2016 2. çeyrek raporuna göre; akıllı telefon pazarında Android %87.6 gibi çok yüksek bir paya sahiptir [1]. Android'in akıllı telefon kullanan kullanıcılar arasında popüler olması ile birlikte açık kaynaklı bir yapıya sahip olması ve markete uygulama yüklenirken detaylı olarak kötücül yazılım incelenmesi yapılmadığından dolayı Android platformu kötü niyetli kişilerin bir numaralı hedefi haline gelmiştir. Android market ve diğer alternatif Android marketlerde kötü niyetli uygulamaların sayısı her geçen gün artmaktadır. G Data'nın 2015 1. Çeyrek raporuna göre; kötücül yazılımların %50.3 finansal amaçlıdır [2]. Finansal amaçlı olmasındaki neden Avrupa kıtasındaki kullanıcıların %41'nin banka işlemlerini akıllı telefonlarını kullanarak yapmasıdır [2]. Bu yüzden Android marketlerde bulunan uygulamaların kötücül olup olmadığını tespit etmek için etkin kötücül yazılım tespiti yapan sistemlere ihtiyaç vardır. Bu çerçevede bu çalışmada kötücül yazılım tespit sistemleri anlatılmıştır.

Prevalence of the N-Acetyltransferase (NAT2 gene polymorphism 282C>T in Peruvian population and health implications

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Salazar-Granara Alberto

2016-03-01

Full Text Available Objective: To determine the frequency of the C282T polymorphism of the NAT2 gene (N acetyltransferase in Peruvian populations. Field work, focused on exploring genetic risk factor in Peruvian populations, which has influence in the response to drugs and malignancies aetiology. Material and Methods: Cross-sectional study. 166 voluntaries from Lima, Lambayeque, Apurimac, Puno, San Martin, Amazonas and Loreto were enrolled. The sampling was done by convenience and it was use the RFLP-PCR conventional technique was used. Results: The allele frequency were 54% (n=126 for C282 and 46% (n=106 for T282. For the T allele, by its orign , stand out 2 those which origins were Lima 42% (n=25, Amazonas 47% (n=16, San Martin 74% (n=28 and Apurimac 50% (n=13 (X , p>0.05. A global genotype frequency were 26.7% (n=31 for C282/C282, 56.0% (n=65 for C282/T282 and 17.2% (n=20 for T282/T282 (Hardy Weinberg Test p>0.05. By origin, Puno presented allelic imbalance (Hardy Weinberg test p0.05. Conclusion: The overall frequency of NAT2 allele T282 was 46%; San Martin had the highest prevalence (74%. The T282 allele is linked to neoplastic diseases and adverse reactions to anti-TB drugs, these results will be used for the application of pharmacogenetics in Peru

Usefulness of Cytokeratin-18M65 in Diagnosing Non-Alcoholic Steatohepatitis in Japanese Population.

Science.gov (United States)

Hasegawa, Yutaka; Kim, Soo Ryang; Hatae, Takashi; Ohta, Mitsuhiro; Fujinami, Aya; Sugimoto, Kayo; Kim, Ke Ih; Imoto, Susumu; Tohyama, Madoka; Kim, Soo Ki; Ikura, Yoshihiro; Kudo, Masatoshi

2015-10-01

The aim of this study was to evaluate cytokeratin-18M65 (CK-18M65) for distinguishing between simple steatosis (SS) and non-alcoholic steatohepatitis (NASH) against healthy individuals (HIs) in Japanese population. The serum from 24 HIs, 21 patients with SS and 20 patients with NASH were examined. Serum CK-18M65 was measured by enzyme-linked immunosorbent assay. Aspartate aminotransferase was significantly different between NASH patients and HIs with p fashion in HIs and also in SS and NASH patients. Multivariate logistic regression analysis revealed that NASH could be diagnosed with the use of CK-18M65 alone (p = 0.0285, OR 1.0038, 95% CI 1.0004-1.0073). At the optimal cut-off level of 548 U/l, CK-18M65 had an AUC value of 0.7369, 60.00% sensitivity and 85.70% specificity. In patients with NASH, no significant difference was observed between low fibrosis (Stage 0-1, 794.30 ± 454.41, n = 10) and high fibrosis (Stage 2-3, 809.70 ± 641.43, n = 10; p = 0.5967) and between slight steatosis (Japanese population. © 2015 S. Karger AG, Basel.

Intercoalescence time distribution of incomplete gene genealogies in temporally varying populations, and applications in population genetic inference.

Science.gov (United States)

Chen, Hua

2013-03-01

Tracing back to a specific time T in the past, the genealogy of a sample of haplotypes may not have reached their common ancestor and may leave m lineages extant. For such an incomplete genealogy truncated at a specific time T in the past, the distribution and expectation of the intercoalescence times conditional on T are derived in an exact form in this paper for populations of deterministically time-varying sizes, specifically, for populations growing exponentially. The derived intercoalescence time distribution can be integrated to the coalescent-based joint allele frequency spectrum (JAFS) theory, and is useful for population genetic inference from large-scale genomic data, without relying on computationally intensive approaches, such as importance sampling and Markov Chain Monte Carlo (MCMC) methods. The inference of several important parameters relying on this derived conditional distribution is demonstrated: quantifying population growth rate and onset time, and estimating the number of ancestral lineages at a specific ancient time. Simulation studies confirm validity of the derivation and statistical efficiency of the methods using the derived intercoalescence time distribution. Two examples of real data are given to show the inference of the population growth rate of a European sample from the NIEHS Environmental Genome Project, and the number of ancient lineages of 31 mitochondrial genomes from Tibetan populations. © 2013 Blackwell Publishing Ltd/University College London.

Síndrome del túnel del tarso: Normalización de una técnica diagnóstica electrofisiológica

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Juan Pablo Valderrama

1994-10-01

Full Text Available EI síndrome del túnel del tarso es una entidad frecuente en la población adulta, pero infrecuentemente documentada. En su diagnóstico los estudios de neuroconducción han demostrado ser importantes para confirmar su presencia y evaluar su severidad. La presente investigación, hace un análisis prospectivo de los hallazgos de neuroconducción motora de los nervios plantares interno y externo, encontrados en 32 individuos asintomáticos. Se verifica con el objeto de estandarizar valores de referencia para nuestro medio, aplicando la técnica de Felsenthal, para discriminar entre una lesión a nivel del túnel del tarso, lesiones aisladas de los nervios plantares o neuropatías distales por otra causa. Los resultados obtenidos mostraron una latencia motora promedio a través del túnel para el nervio plantar interno de 2.35 ms (rango O.64ms - 4.09ms y para el nervio plantar externo de 2.32ms (rango O.29ms - 3.77ms, también se establecieron velocidades de conducción proximal del nervio tibial para descartar anormalidades en su trayecto a través de la pierna, que pudieran invalidar los datos obtenidos distalmente.

Flipped SU(5) predicts {delta}T/T

Energy Technology Data Exchange (ETDEWEB)

Kyae, Bumseok [School of Physics, Korea Institute for Advanced Study, 207-43, Cheongnyangni-Dong, Dongdaemun-Gu, Seoul 130-722 (Korea, Republic of)]. E-mail: bkyae@kias.re.kr; Shafi, Qaisar [Bartol Research Institute, Department of Physics and Astronomy, University of Delaware, Newark, DE 19716 (United States)]. E-mail: shafi@bartol.udel.edu

2006-04-20

We discuss hybrid inflation in supersymmetric flipped SU(5) model such that the cosmic microwave anisotropy {delta}T/T is essentially proportional to (M/M{sub P}){sup 2}, where M denotes the symmetry breaking scale and M{sub P} (=2.4x10{sup 18} GeV) is the reduced Planck mass. The magnitude of M determined from {delta}T/T measurements can be consistent with the value inferred from the evolution of SU(3) and SU(2) gauge couplings. In other words, one could state that flipped SU(5) predicts (more precisely 'postdicts') {delta}T/T. The scalar spectral index n{sub s}=0.993+/-0.007, the scalar to tensor ratio satisfies r-bar 10{sup -6}, while dn{sub s}/dlnk-bar 4x10{sup -4}.

Analysis of glutathione S-transferase (M1, T1 and P1) gene ...

African Journals Online (AJOL)

Glutathione S-transferase enzymes are active in detoxifying a wide number of endogenous and exogenous chemical carcinogens and subsequently, are crucial in protecting the DNA. Several studies show some differences in association of glutathione S-transferase M1, T1 and P1 genetic polymorphisms with the risk of ...

Pesquisa de anticorpos IgM anti-Toxoplasma gondii por meio de técnica imunoenzimática reversa

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José Roberto Mineo

1986-02-01

Full Text Available Um teste imunoenzimático reverso foi padronizado utilizando-se como fase sólida, microplacas de polivinil sensibilizadas com anticorpos anti-IgM.7 Estas foram incubadas seqüencialmente com alíquotas de soros de pacientes com suspeita de toxoplasmose aguda, antígeno solúvel de Toxoplasma gondii, conjugado peroxidase F (ab'2 anti-toxoplasma e substrato enzimático. A atividade enzimática foi determinada por leitura espectrofotométrica, considerando-se como títulos dos soros a máxima diluição fornecendo valores de absorbância maiores que os obtidos com a menor diluição do soro padrão não-reativo. Em 69 amostras de soros de pacientes com toxoplasmose aguda, a média geométrica dos títulos no teste ELISA-Reverso IgM foi superior à de todos os outros testes para anticorpos IgM, não se observando resultados negativos falsos devidos a altos títulos de IgG específica. Não foi encontrada, também, reatividade cruzada em nenhuma das 104 amostras de soros de pacientes com outras patologias, inclusive em amostras contendo fator reumatóide IgM.

Copy number variation in glutathione-S-transferase T1 and M1 predicts incidence and 5-year survival from prostate and bladder cancer, and incidence of corpus uteri cancer in the general population

DEFF Research Database (Denmark)

Nørskov, M S; Frikke-Schmidt, R; Bojesen, S E

2011-01-01

Glutathione-S-transferase T1 (GSTT1) and GSTM1 detoxify carcinogens and thus potentially contribute to inter-individual susceptibility to cancer. We determined the ability of GST copy number variation (CNV) to predict the risk of cancer in the general population. Exact copy numbers of GSTT1 and G...

The M142T mutation causes B3 phenotype: three cases and an in vitro expression study.

Science.gov (United States)

Cho, Duck; Shin, Dong-Jun; Yazer, Mark Harris; Ihm, Chun-Hwa; Hur, Young-Moon; Kee, Seung-Jung; Kim, Soo-Hyun; Shin, Myung-Geun; Shin, Jong-Hee; Suh, Soon-Pal; Ryang, Dong-Wook

2010-02-01

The B3 phenotype is the most common B subtype in Korea. The B305 allele (425 T>C, M142T) was first reported in 2 Chinese individuals; however, it has not yet been reported in the Koreans, and the impact of the M142T mutation on the expression of the B3 phenotype has also not been studied. To resolve an ABO discrepancy between a group O neonate and her group O father and A(1)B(3) mother, blood samples from these individuals and other family members were referred to our laboratory for ABO gene analysis. The B305 allele was discovered in the neonate (B305/O01), her mother (A102/ B305), and her maternal aunt (B305/O02), while her father was typed as O01/O02. Transient transfection experiments were performed in HeLa cells using the B305 allele synthesized by site-directed mutagenesis; flow cytometric analysis revealed that this transfect expressed 35.5% of the total B antigen produced by the B101 allele transfect. For comparison, Bx01 allele transfects were also created, and they expressed 11.4% of the total B antigen expressed on the surface of B101 transfects. These experiments demonstrate that the M142T (425 T>C) mutation is responsible for the B subtype phenotype produced by the B305 allele.

Asymptotic distributions of coalescence times and ancestral lineage numbers for populations with temporally varying size.

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Chen, Hua; Chen, Kun

2013-07-01

The distributions of coalescence times and ancestral lineage numbers play an essential role in coalescent modeling and ancestral inference. Both exact distributions of coalescence times and ancestral lineage numbers are expressed as the sum of alternating series, and the terms in the series become numerically intractable for large samples. More computationally attractive are their asymptotic distributions, which were derived in Griffiths (1984) for populations with constant size. In this article, we derive the asymptotic distributions of coalescence times and ancestral lineage numbers for populations with temporally varying size. For a sample of size n, denote by Tm the mth coalescent time, when m + 1 lineages coalesce into m lineages, and An(t) the number of ancestral lineages at time t back from the current generation. Similar to the results in Griffiths (1984), the number of ancestral lineages, An(t), and the coalescence times, Tm, are asymptotically normal, with the mean and variance of these distributions depending on the population size function, N(t). At the very early stage of the coalescent, when t → 0, the number of coalesced lineages n - An(t) follows a Poisson distribution, and as m → n, $$n\\left(n-1\\right){T}_{m}/2N\\left(0\\right)$$ follows a gamma distribution. We demonstrate the accuracy of the asymptotic approximations by comparing to both exact distributions and coalescent simulations. Several applications of the theoretical results are also shown: deriving statistics related to the properties of gene genealogies, such as the time to the most recent common ancestor (TMRCA) and the total branch length (TBL) of the genealogy, and deriving the allele frequency spectrum for large genealogies. With the advent of genomic-level sequencing data for large samples, the asymptotic distributions are expected to have wide applications in theoretical and methodological development for population genetic inference.

Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry.

Science.gov (United States)

Pavlova, Ivelina; Milanova, Aneliya; Danova, Svetla; Fink-Gremmels, Johanna

2016-12-01

Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1 and LEAP-2 mRNA was investigated in Ross 308 chickens. One-day-old chicks (n = 24) were allocated to following groups: control (without treatment); group treated with probiotics via feed; group treated with a combination of probiotics and enrofloxacin; and a group given enrofloxacin only. The drug was administered at a dose of 10 mg kg -1 , via drinking water for 5 days. Samples from liver, duodenum and jejunum were collected 126 h after the start of the treatment. Expression levels of PepT1 and LEAP-2 were determined by real-time polymerase chain reaction and were statistically evaluated by Mann-Whitney test. Enrofloxacin administered alone or in combination with probiotics provoked a statistically significant up-regulation of PepT1 mRNA levels in the measured organ sites. These changes can be attributed to a tendency of improvement in utilization of dietary peptide and in body weight gain. LEAP-2 mRNA expression levels did not change significantly in enrofloxacin-treated chickens in comparison with control group.

Crystallization and preliminary crystallographic analysis of tRNA (m{sup 7}G46) methyltransferase from Escherichia coli

Energy Technology Data Exchange (ETDEWEB)

Liu, Qi; Gao, Yang; Yang, Weili; Zhou, Huihao; Gao, Yongxiang; Zhang, Xiao; Teng, Maikun, E-mail: mkteng@ustc.edu.cn; Niu, Liwen, E-mail: mkteng@ustc.edu.cn [Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027 (China); Key Laboratory of Structural Biology, Chinese Academy of Sciences, 96 Jinzhai Road, Hefei, Anhui 230027 (China)

2008-08-01

tRNA (m{sup 7}G46) methyltransferase from E. coli was overexpressed, purified and crystallized. Diffraction data were collected to 2.04 Å resolution. Transfer RNA (tRNA) (m{sup 7}G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-l-methionine (SAM) as the methyl-group donor to catalyze the formation of N{sup 7}-methylguanosine (m{sup 7}G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His{sub 6} tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P2{sub 1}.

Draft genome sequence of Pseudomonas sp. strain M47T1, carried by Bursaphelenchus xylophilus isolated from Pinus pinaster.

Science.gov (United States)

Proença, Diogo Neves; Espírito Santo, Christophe; Grass, Gregor; Morais, Paula V

2012-09-01

The draft genome sequence of Pseudomonas sp. strain M47T1, carried by the Bursaphelenchus xylophilus pinewood nematode, the causative agent of pine wilt disease, is presented. In Pseudomonas sp. strain M47T1, genes that make this a plant growth-promoting bacterium, as well as genes potentially involved in nematotoxicity, were identified.

Nutrient sensing via mTOR in T cells maintains a tolerogenic microenvironment

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Duncan eHowie

2014-08-01

Full Text Available We have proposed that tolerance can be maintained by the induction, by Treg cells, of a tolerogenic microenvironment within tolerated tissues that inhibits effector cell activity but which supports the generation of further Treg cells by infectious tolerance. Two important components of this tolerogenic microenvironment depend on metabolism and nutrient sensing. The first is due to the up-regulation of multiple enzymes that consume essential amino acids (EAAs, which are sensed in naive T cells primarily via inhibition of the mTOR pathway, which in turn encourages their further differentiation into foxp3+ Treg cells. The second mechanism is the metabolism of extracellular ATP to adenosine by the ectoenzymes CD39 and CD73. These two enzymes are constitutively co-expressed on Treg cells, but can also be induced on a wide variety of cell types by TGFbeta and the adenosine generated can be shown to be a potent inhibitor of T cell proliferation. This review will focus on mechanisms of nutrient sensing in T cells, how these are integrated with TCR and cytokine signals via the mnTOR pathway, and what impact this has on intracellular metabolism and subsequently the control of differentiation into different effector or regulatory T cell subsets.

Selfridge ANGB, Michigan. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

Science.gov (United States)

1982-04-20

umuwuommuwrU(@m~l 77 Li ’AL CLIMATOLOY FRANCH -~~iTAC- A’. 4EATHER SERVICE/MAC WEATHER CONDITIONS .’..377 3,ELFRIDGE ANG8 MI 69-70,74-81 FEB STATION STATION NAME...I TABULM~IOX CEILING) > 1800 a 1500 9. 2t 1200 *1 ~000 2t 700 a:600_ _ 2t 400 9. 2t 300 z 00 98. ECANFLE # 1 food ceiling values Independently of...iii I.2 41,01- . AL CLIMATOLOGY FRANCH ,2;TAC SKY COVER A

Kesklinna kolimine tõi Renault' müüjale loodetust suurema edu / Tõnu Tramm

Index Scriptorium Estoniae

Tramm, Tõnu, 1968-

2005-01-01

TOP-i 2. koht. Renault'sid müüva Autori AS-i juht Vello Tamm peab ettevõtte edu aluseks kolimist kesklinna ning senise töö analüüsimist ja uute eesmärkide seadmist. Diagramm: Majandusnäitajad

Pulsed time-of-flight refractometry measurements of the electron density in the T-11M tokamak

International Nuclear Information System (INIS)

Petrov, A.A.; Petrov, V.G.; Malyshev, A.Yu.; Markov, V.K.; Babarykin, A.V.

2002-01-01

A new method for measuring the plasma density in magnetic confinement systems - pulsed time-of-flight refractometry - is developed and tested experimentally in the T-11M tokamak. The method is based on the measurements of the time delay of short (with a duration of several nanoseconds) microwave pulses propagating through the plasma. When the probing frequency is much higher than the plasma frequency, the measured delay in the propagation time is proportional to the line-averaged electron density regardless of the density profile. A key problem in such measurements is the short time delay of the pulse in the plasma (∼1 ns or less for small devices) and, consequently, low accuracy of the measurements of the average density. Various methods for improving the accuracy of such measurements are proposed and implemented in the T-11M experiments. The measurements of the line-averaged density in the T-11M tokamak in the low-density plasma regime are performed. The results obtained agree satisfactorily with interferometric data. The measurement errors are analyzed, and the possibility of using this technique to measure the electron density profile and the position of the plasma column is discussed

Defective immunoregulatory T-cell function in chronic lymphocytic leukemia

International Nuclear Information System (INIS)

Han, T.; Ozer, H.; Henderson, E.S.; Dadey, B.; Nussbaum-Blumenson, A.; Barcos, M.

1981-01-01

Chronic lymphocytic leukemia (CLL) of B-cell origin results in the malignant proliferation of small immunoglobulin-bearing lymphocytes. There is currently a controversy in the literature regarding both the ability of this leukemic population to differentiate into mature plasma cells, as well as the ability of apparently normal T cells from these patients to regulate allogeneic B-cell differentiation. In the present study we have examined the lymphocytes of CLL patients in various clinical stages of their disease and with different surface phenotypes of their leukemic B-cell population. Our results show that leukemic CLL B cells from all 20 patients (including one patient with a monoclonal IgM paraprotein and another with a monoclonal IgG paraprotein) are incapable of further differentiation even in the absence of suppressor T cells and the presence of helper T lymphocytes. This lack of capacity to differentiate is unaffected by clinical stage, by therapy, or by the phenotype of the malignant population. Since the leukemic B population did not suppress normal allogeneic B-cell differentiation, the maturation deficit is evidently intrinsic to the leukemic clone rather than a result of activity of non-T suppressor cells. T helper function was also variably depressed in the blood of some patients with CLL, and this depression did not correlate with clinical stage, with therapy, or with the degree of lymphocytosis. Dysfunction of radiosensitive T suppressor cells was found to be the most consistent regulatory deficit of CLL T cells. Each of 11 patients whose leukemic cell population was of the μdelta, μα, or μ phenotype had both helper and suppressor cell defects

Why patients visit their doctors: assessing the most prevalent conditions in a defined American population.

Science.gov (United States)

St Sauver, Jennifer L; Warner, David O; Yawn, Barbara P; Jacobson, Debra J; McGree, Michaela E; Pankratz, Joshua J; Melton, L Joseph; Roger, Véronique L; Ebbert, Jon O; Rocca, Walter A

2013-01-01

To describe the prevalence of nonacute conditions among patients seeking health care in a defined US population, emphasizing age, sex, and ethnic differences. The Rochester Epidemiology Project (REP) medical records linkage system was used to identify all residents of Olmsted County, Minnesota, on April 1, 2009, who had consented to review of their medical records for research (142,377 patients). We then electronically extracted all International Classification of Diseases, Ninth Revision codes noted in the records of these patients by any health care institution between January 1, 2005, and December 31, 2009. We grouped International Classification of Diseases, Ninth Revision codes into clinical classification codes and then into 47 broader disease groups associated with health-related quality of life. Age- and sex-specific prevalence was estimated by dividing the number of individuals within each group by the corresponding age- and sex-specific population. Patients within a group who had multiple codes were counted only once. We included a total of 142,377 patients, 75,512 (53%) of whom were female. Skin disorders (42.7%), osteoarthritis and joint disorders (33.6%), back problems (23.9%), disorders of lipid metabolism (22.4%), and upper respiratory tract disease (22.1%, excluding asthma) were the most prevalent disease groups in this population. Ten of the 15 most prevalent disease groups were more common in women in almost all age groups, whereas disorders of lipid metabolism, hypertension, and diabetes were more common in men. Additionally, the prevalence of 7 of the 10 most common groups increased with advancing age. Prevalence also varied across ethnic groups (whites, blacks, and Asians). Our findings suggest areas for focused research that may lead to better health care delivery and improved population health. Copyright © 2013 Mayo Foundation for Medical Education and Research. Published by Elsevier Inc. All rights reserved.

Challenges Facing ZnO and GaN: Facts and Myths. Held in Glen Allen, Virginia on 18-19 October 2007

Science.gov (United States)

2007-10-01

nitrides”, Acta Physica Polonica . 87 (1995) 295. 5 M. Leszczynski, I. Grzegory, H. Teisseyre, T. Suski, M. Bockowski, J. Jun, J.M. Baranowski, S...Nemeth, J. Nause, J. Xie, B. Hertog, and A. Osinsky; “Luminescence properties of defects in ZnO”; Physica B; 401-402, 358, (2007). 67 R. Dingle...O (TM: V, Cr, Mn, Fe, Co, and Ni) in the self-interaction-corrected calculations”; Physica B; 376–377, 647, (2006). 146

MELAS syndrome associated with a new mitochondrial tRNA-Val gene mutation (m.1616A>G).

Science.gov (United States)

Toyoshima, Yuka; Tanaka, Yuji; Satomi, Kazuo

2017-09-11

We describe the case of a 40-year-old-man with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) syndrome, with cardiomyopathy and severe heart failure. He had a mitochondrial transfer RNA (tRNA) mutation (m.1616A>G) of the (tRNA-Val) gene, and it was not found in MELAS syndrome ever before. The presence of this newly observed tRNA-Val mutation (m.1616A>G) may induce multiple respiratory chain enzyme deficiencies and contribute to MELAS syndrome symptoms that are associated with mitochondrial DNA (mtDNA) mutations. We report that the pathognomonic symptom in MELAS syndrome caused by this newly observed mtDNA mutation may be rapid progression of cardiomyopathy and severe heart failure. © BMJ Publishing Group Ltd (unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Native T-1 reference values for nonischemic cardiomyopathies and populations with increased cardiovascular risk : A systematic review and meta-analysis

NARCIS (Netherlands)

van den Boomen, Maaike; Slart, Riemer H J A; Hulleman, Enzo V; Dierckx, Rudi A J O; Velthuis, Birgitta K; van der Harst, Pim; Sosnovik, David E; Borra, Ronald J H; Prakken, Niek H J

BACKGROUND: Although cardiac MR and T1 mapping are increasingly used to diagnose diffuse fibrosis based cardiac diseases, studies reporting T1 values in healthy and diseased myocardium, particular in nonischemic cardiomyopathies (NICM) and populations with increased cardiovascular risk, seem

Improved fat-suppression homogeneity with mDIXON turbo spin echo (TSE) in pediatric spine imaging at 3.0 T.

Science.gov (United States)

Pokorney, Amber L; Chia, Jonathan M; Pfeifer, Cory M; Miller, Jeffrey H; Hu, Houchun H

2017-11-01

Background Robust fat suppression remains essential in clinical MRI to improve tissue signal contrast, minimize fat-related artifacts, and enhance image quality. Purpose To compare fat suppression between mDIXON turbo spin echo (TSE) and conventional frequency-selective and inversion-recovery methods in pediatric spine MRI. Material and Methods Images from T1-weighted (T1W) and T2-weighted (T2W) TSE sequences coupled with conventional methods and the mDIXON technique were compared in 36 patients (5.8 ± 5.4 years) at 3.0 T. Images from 42 pairs of T1W (n = 16) and T2W (n = 26) scans were acquired. Two radiologists reviewed the data and rated images using a three-point scale in two categories, including the uniformity of fat suppression and overall diagnostic image quality. The Wilcoxon rank-sum test was used to compare the scores. Results The Cohen's kappa coefficient for inter-rater agreement was 0.69 (95% confidence interval [CI], 0.56-0.83). Images from mDIXON TSE were considered superior in fat suppression ( P 3.0 T and should be considered as a permanent replacement of traditional methods, in particular frequency-selective techniques.

Apgrozījuma prognozēšana ar mākslīgo neironu tīklu palīdzību

OpenAIRE

Puškina, Tatjana

2012-01-01

Bakalaura darba pētījumu ietvaros tika analizēta mākslīgo neironu tīklu programmas izvēle Latvijas uzņēmuma „Pasaules Optika” apgrozījuma prognozēšanai, kā arī veikta tā praktiskā pielietošana. Darbs sastāv no trim nodaļām, kurās tiek izskatītas problēmas, kas ir iespējams risināt ar mākslīgo neironu tiklu palīdzību, izmantojot Neuro XL programmu. Salīdzinājumā ar citām mākslīgā intelekta risinošam datu apstrādes programmām tiek novērtēts Neuro XL programmas priekšrocības, tiek imitēti ikgadē...

Frequency of adult type-associated lactase persistence LCT-13910C/T genotypes in the Czech/Slav and Czech Roma/Gypsy populations

Directory of Open Access Journals (Sweden)

Jaroslav A. Hubácek

2017-05-01

Full Text Available Abstract Lactase non-persistence (leading to primary lactose intolerance is a genetically dependent inability to digest lactose in adulthood. As part of the human adaptation to dairying, the human lactase LCT-13910C/T mutation (which propagates adult expression of lactase developed, spread and participated in the adaptation to dairying. This variant is associated with lactase activity persistence, and its carriers are able to digest lactose. We compared the frequencies of lactase 13910C/T (rs4988235 genotypes in Czechs/Slavs (N = 288 and Czech Gypsies/Roma (N = 300, two ethnically different groups where this polymorphism has not yet been analysed. Allelic frequencies significantly differed between the populations (p < 0.0001. In Czechs/Slavs, the lactase persistence T allele was present in 76% of the individuals, which is in agreement with frequencies among geographically neighbouring populations. In the Czech Gypsy/Roma population, only 27% of the adults were carriers of at least one lactase persistence allele, similar to the Indian population. In agreement with this result, dairy product consumption was reported by 70.5% of Czechs/Slavs and 39.0% of the Czech Gypsy/Roma population. Both in the Czech Gypsy/Roma and in the Czech/Slavs populations, the presence of carriers of the lactase persistence allele was similar in subjects self-reporting the consumption of unfermented/fresh milk, in comparison to the others.

Análise do método de simulação de desempenho térmico da norma NBR 15.575

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Márcio José SORGATO

2014-09-01

Full Text Available As normas de desempenho de edificações avaliam e regulamentam o setor da construção civil, garantindo um padrão mínimo de qualidade e desempenho das edificações residenciais. O artigo tem como objetivo avaliar o método de simulação do desempenho térmico de edificações residenciais da NBR 15575. Observou-se que o método da norma apresenta limitações, avaliando o desempenho da edificação através de dias típicos de verão e inverno. Como proposta, foi desenvolvido e encaminhado um novo método de simulação durante a consulta pública da NBR 15575. A proposta do novo método busca avaliar o desempenho térmico da edificação através dos graus-hora de resfriamento e aquecimento. Os resultados da comparação entre estes dois métodos foram analisados, permitindo observar que o método de simulação da NBR 15575 atende ao desempenho térmico mínimo quando comparado com o sistema construtivo com paredes de concreto de 5 cm de espessura. Já com a aplicação do método proposto, observou-se que a edificação apresentou um desempenho térmico insatisfatório para o sistema construtivo analisado. Palavras-Chave: simulação computacional, desempenho térmico, edificações residências.

mTOR Complex Signaling through the SEMA4A-Plexin B2 Axis Is Required for Optimal Activation and Differentiation of CD8+ T Cells.

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Ito, Daisuke; Nojima, Satoshi; Nishide, Masayuki; Okuno, Tatsusada; Takamatsu, Hyota; Kang, Sujin; Kimura, Tetsuya; Yoshida, Yuji; Morimoto, Keiko; Maeda, Yohei; Hosokawa, Takashi; Toyofuku, Toshihiko; Ohshima, Jun; Kamimura, Daisuke; Yamamoto, Masahiro; Murakami, Masaaki; Morii, Eiichi; Rakugi, Hiromi; Isaka, Yoshitaka; Kumanogoh, Atsushi

2015-08-01

Mammalian target of rapamycin (mTOR) plays crucial roles in activation and differentiation of diverse types of immune cells. Although several lines of evidence have demonstrated the importance of mTOR-mediated signals in CD4(+) T cell responses, the involvement of mTOR in CD8(+) T cell responses is not fully understood. In this study, we show that a class IV semaphorin, SEMA4A, regulates CD8(+) T cell activation and differentiation through activation of mTOR complex (mTORC) 1. SEMA4A(-/-) CD8(+) T cells exhibited impairments in production of IFN-γ and TNF-α and induction of the effector molecules granzyme B, perforin, and FAS-L. Upon infection with OVA-expressing Listeria monocytogenes, pathogen-specific effector CD8(+) T cell responses were significantly impaired in SEMA4A(-/-) mice. Furthermore, SEMA4A(-/-) CD8(+) T cells exhibited reduced mTORC1 activity and elevated mTORC2 activity, suggesting that SEMA4A is required for optimal activation of mTORC1 in CD8(+) T cells. IFN-γ production and mTORC1 activity in SEMA4A(-/-) CD8(+) T cells were restored by administration of recombinant Sema4A protein. In addition, we show that plexin B2 is a functional receptor of SEMA4A in CD8(+) T cells. Collectively, these results not only demonstrate the role of SEMA4A in CD8(+) T cells, but also reveal a novel link between a semaphorin and mTOR signaling. Copyright © 2015 by The American Association of Immunologists, Inc.

A comparison of QuantStudio™ 3D Digital PCR and ARMS-PCR for measuring plasma EGFR T790M mutations of NSCLC patients.

Science.gov (United States)

Feng, Qin; Gai, Fei; Sang, Yaxiong; Zhang, Jie; Wang, Ping; Wang, Yue; Liu, Bing; Lin, Dongmei; Yu, Yang; Fang, Jian

2018-01-01

The AURA3 clinical trial has shown that advanced non-small cell lung cancer (NSCLC) patients with EGFR T790M mutations in circulating tumor DNA (ctDNA) could benefit from osimertinib. The aim of this study was to assess the usefulness of QuantStudio™ 3D Digital PCR System platform for the detection of plasma EGFR T790M mutations in NSCLC patients, and compare the performances of 3D Digital PCR and ARMS-PCR. A total of 119 Chinese patients were enrolled in this study. Mutant allele frequency of plasma EGFR T790M was detected by 3D Digital PCR, then 25 selected samples were verified by ARMS-PCR and four of them were verified by next generation sequencing (NGS). In total, 52.94% (69/119) had EGFR T790M mutations detected by 3D Digital PCR. In 69 positive samples, the median mutant allele frequency (AF) was 1.09% and three cases presented low concentration (AF Digital PCR) was identified as T790M- by ARMS-PCR. Four samples were identified as T790M+ by both NGS and 3D Digital PCR, and typically three samples (3/4) presented at a low ratio (AF Digital PCR is a novel method with high sensitivity and specificity to detect EGFR T790M mutation in plasma.

ULTRAVIOLET-BRIGHT STELLAR POPULATIONS AND THEIR EVOLUTIONARY IMPLICATIONS IN THE COLLAPSED-CORE CLUSTER M15

International Nuclear Information System (INIS)

Haurberg, Nathalie C.; Lubell, Gabriel M. G.; Cohn, Haldan N.; Lugger, Phyllis M.; Anderson, Jay; Cool, Adrienne M.; Serenelli, Aldo M.

2010-01-01

We performed deep photometry of the central region of the Galactic globular cluster M15 from archival Hubble Space Telescope data taken on the High Resolution Channel and Solar Blind Channel of the Advanced Camera for Surveys. Our data set consists of images in far-UV (FUV 140 ; F140LP), near-UV (NUV 220 ; F220W), and blue (B 435 ; F435W) filters. The addition of an optical filter complements previous UV work on M15 by providing an additional constraint on the UV-bright stellar populations. Using color-magnitude diagrams (CMDs), we identified several populations that arise from non-canonical evolution including candidate blue stragglers, extreme horizontal branch (HB) stars, blue hook (BHk) stars, cataclysmic variables (CVs), and helium-core white dwarfs (He WDs). Due to preliminary identification of several He WD and BHk candidates, we add M15 as a cluster containing an He WD sequence and suggest it be included among clusters with a BHk population. We also investigated a subset of CV candidates that appear in the gap between the main sequence (MS) and WDs in FUV 140 -NUV 220 but lie securely on the MS in NUV 220 -B 435 . These stars may represent a magnetic CV or detached WD-MS binary population. Additionally, we analyze our candidate He WDs using model cooling sequences to estimate their masses and ages and investigate the plausibility of thin versus thick hydrogen envelopes. Finally, we identify a class of UV-bright stars that lie between the HB and WD cooling sequences, a location not usually populated on cluster CMDs. We conclude these stars may be young, low-mass He WDs.

Role of the Akt/mTOR signaling pathway in human papillomavirus-associated nasal and sinonasal inverted papilloma.

Science.gov (United States)

Liu, Yongliang; Duan, Lihua; Tian, Jie; Song, Daoliang; Zhang, Min; Zhao, Shenlin; Yin, Zhaofu; Xiang, Xinxin; Li, Xuezhong

2017-12-01

Nasal and sinonasal inverted papilloma (NSIP) is a benign tumor in which surface epithelial cells grow downward into the underlying supportive tissue with varying degrees of metaplasia. Human papillomavirus (HPV) has been proposed as the causal agent in the pathogenesis of this disease. Many studies have shown that HPV can activate the Akt/mechanistic target of rapamycin (mTOR) signaling pathway, but the role of this pathway in HPV-associated NSIP is largely unknown. In this study, we enrolled 40 control tissue samples and 80 NSIP tissue samples. HPV genotyping showed that 47 of the 80 examined cases of NSIP were HPV-positive (58.8%), and the most common subtype was HPV11 (20/53, 37.7%). The immunohistochemistry showed statistically significant differences in phosphorylated Akt and phosphorylated S6 ribosomal protein staining among control samples, HPV-positive NSIP and HPV-negative NSIP. The HPV11 L1-L2 plasmid increased the proliferation of normal human nasopharyngeal epithelial NP69-SV40T cells and human nasopharyngeal cancer CNE1 cells. Meanwhile, rapamycin, an mTOR inhibitor, reversed the increased cell proliferation induced by the HPV11 L1-L2 plasmid. Western blot analysis showed that Akt/mTOR/S6 were overexpressed in NP69-SV40T cells and CNE1 cells infected with the HPV11 L1-L2 plasmid. These data demonstrate that HPV promotes cell proliferation through the Akt/mTOR signaling pathway in NSIP. © The Author 2017. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Single Cell Dynamics Causes Pareto-Like Effect in Stimulated T Cell Populations.

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Cosette, Jérémie; Moussy, Alice; Onodi, Fanny; Auffret-Cariou, Adrien; Neildez-Nguyen, Thi My Anh; Paldi, Andras; Stockholm, Daniel

2015-12-09

Cell fate choice during the process of differentiation may obey to deterministic or stochastic rules. In order to discriminate between these two strategies we used time-lapse microscopy of individual murine CD4 + T cells that allows investigating the dynamics of proliferation and fate commitment. We observed highly heterogeneous division and death rates between individual clones resulting in a Pareto-like dominance of a few clones at the end of the experiment. Commitment to the Treg fate was monitored using the expression of a GFP reporter gene under the control of the endogenous Foxp3 promoter. All possible combinations of proliferation and differentiation were observed and resulted in exclusively GFP-, GFP+ or mixed phenotype clones of very different population sizes. We simulated the process of proliferation and differentiation using a simple mathematical model of stochastic decision-making based on the experimentally observed parameters. The simulations show that a stochastic scenario is fully compatible with the observed Pareto-like imbalance in the final population.

Sotsiaaldialoogi ja töötajate kaasatuse mõõtmine = Measurement of social dialogue and involvement of employees / Epp Kallaste

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Kallaste, Epp

2006-01-01

Vaatluse all on kvaliteetse töö kontseptsiooni osa - sotsiaaldialoog ja töösuhted - mõõtmise kolm indikaatorit: ametiühingute liikmelisus, kollektiivlepingute katvus ja streigid ning töösulud Eestis. Diagrammid

Anticipating and addressing workplace static magnetic field effects at levels <0.5 mT.

Science.gov (United States)

Emery, R J; Hopkins, J R; Charlton, M A

2000-11-01

Magnetic resonance, once a research tool limited to the basic sciences, has experienced an increase in popularity due to its unique ability to analyze certain living systems in vivo. Expanding applications in the biomedical sciences have resulted in magnetic sources being located in research institutions nationally. Space and resource limitations sometimes necessitate siting magnetic resonance units in proximity to other institutional operations. For magnetic field shielding and personnel protection considerations, the generally accepted 0.5 mT (milliTesla) limit for implanted cardiac devices is commonly used as the conservative basis for decisions. But the effects of magnetic fields on equipment can be easily observed at levels far below 0.5 mT, often resulting in concern and apprehension on the part of personnel in the surrounding areas. Responding to recurrent worker concerns spawned by noticeable effects on equipment at exposure levels information, practicing radiation safety professionals can better anticipate facility incompatibility issues and improve their responses to worker concerns initiated by observed effects on equipment.

Selection and characterization of T-cell variants lacking molecules involved in T-cell activation (T3 T-cell receptor, T44, and T11): analysis of the functional relationship among different pathways of activation

International Nuclear Information System (INIS)

Moretta, A.; Poggi, A.; Olive, D.; Bottino, C.; Fortis, C.; Pantaleo, G.; Moretta, L.

1987-01-01

A clone of the interleukin 2-producing Jurkat leukemia cell line termed JA3 (surface phenotype, T3 + , Ti + , T44 + , T11 + , T40 + ) has been used to induce and select cell variants lacking surface molecules involved in T-cell activation. Following 200 rad of γ-radiation (1 rad = 0.01 Gy), cells were treated with monoclonal antibodies (mAbs) directed to T3, Ti, T44, or T11 antigen and complement. After growth of the residual cells in culture, negative cells were cloned under limiting conditions. Depending on the specificity of the mAb used for the immunoselection, three groups of variants were obtained. (i) The use of mAbs directed to T3 or Ti resulted in cell variants that expressed the T3 - Ti - T44 + Leu1 + T11 + T40 + 4F2 + HLA class I + surface phenotype. (ii) Immunoselection with anti-T44 mAb resulted in 2 variants that shared the T3 - Ti - T44 - Leu1 - T11 - T40 - 4F2 - HLA class I + phenotype. (iii) Cell treatment with anti-T11 mAb resulted in 15 variants characterized by the lack of T11 antigen expression and of all the other T-cell-specific surface antigens. Therefore, it appears that the different sets of JA3 cell variants, like T cells at discrete stages of intrathymic differentiation, may follow a coordinated expression of surface differentiation antigens. Analysis of the functional responsiveness of the three distinct groups of JA3 cell variants to different stimuli showed that all produced interleukin 2 in response to A23187 calcium ionophore plus phorbol 12-myristate 13-acetate

Emergency Department Waiting Times (EDWaT): A Patient Flow Management and Quality of Care Rating mHealth Application.

Science.gov (United States)

Househ, Mowafa; Yunus, Faisel

2014-01-01

Saudi hospital emergency departments (ED) have suffered from long waiting times, which have led to a delay in emergency patient care. The increase in the population of Saudi Arabia is likely to further stretch the healthcare services due to overcrowding leading to decreased healthcare quality, long patient waits, patient dissatisfaction, ambulance diversions, decreased physician productivity, and increased frustration among medical staff. This will ultimately put patients at risk for poor health outcomes. Time is of the essence in emergencies and to get to an ED that has the shortest waiting time can mean life or death for a patient, especially in cases of stroke and myocardial infarction. In this paper, we present our work on the development of a mHealth Application - EDWaT - that will: provide patient flow information to the emergency medical services staff, help in quick routing of patients to the nearest hospital, and provide an opportunity for patients to review and rate the quality of care received at an ED, which will then be forwarded to ED services administrators. The quality ratings will help patients to choose between two EDs with the same waiting time and distance from their location. We anticipate that the use of EDWaT will help improve ED wait times and the quality of care provision in Saudi hospitals EDs.

Preexisting CD4+ T-cell immunity in human population to avian influenza H7N9 virus: whole proteome-wide immunoinformatics analyses.

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Venkata R Duvvuri

Full Text Available In 2013, a novel avian influenza H7N9 virus was identified in human in China. The antigenically distinct H7N9 surface glycoproteins raised concerns about lack of cross-protective neutralizing antibodies. Epitope-specific preexisting T-cell immunity was one of the protective mechanisms in pandemic 2009 H1N1 even in the absence of cross-protective antibodies. Hence, the assessment of preexisting CD4+ T-cell immunity to conserved epitopes shared between H7N9 and human influenza A viruses (IAV is critical. A comparative whole proteome-wide immunoinformatics analysis was performed to predict the CD4+ T-cell epitopes that are commonly conserved within the proteome of H7N9 in reference to IAV subtypes (H1N1, H2N2, and H3N2. The CD4+ T-cell epitopes that are commonly conserved (∼ 556 were further screened against the Immune Epitope Database (IEDB to validate their immunogenic potential. This analysis revealed that 45.5% (253 of 556 epitopes are experimentally proven to induce CD4+ T-cell memory responses. In addition, we also found that 23.3% of CD4+ T-cell epitopes have ≥ 90% of sequence homology with experimentally defined CD8+ T-cell epitopes. We also conducted the population coverage analysis across different ethnicities using commonly conserved CD4+ T-cell epitopes and corresponding HLA-DRB1 alleles. Interestingly, the indigenous populations from Canada, United States, Mexico and Australia exhibited low coverage (28.65% to 45.62% when compared with other ethnicities (57.77% to 94.84%. In summary, the present analysis demonstrate an evidence on the likely presence of preexisting T-cell immunity in human population and also shed light to understand the potential risk of H7N9 virus among indigenous populations, given their high susceptibility during previous pandemic influenza events. This information is crucial for public health policy, in targeting priority groups for immunization programs.

The p.T191M mutation of the CBS gene is highly prevalent among homocystinuric patients from Spain, Portugal and South America.

Science.gov (United States)

Urreizti, Roser; Asteggiano, Carla; Bermudez, Marta; Córdoba, Alfonso; Szlago, Marina; Szlago, Mariana; Grosso, Carola; de Kremer, Raquel Dodelson; Vilarinho, Laura; D'Almeida, Vania; Martínez-Pardo, Mercedes; Peña-Quintana, Luís; Dalmau, Jaime; Bernal, Jaime; Briceño, Ignacio; Couce, María Luz; Rodés, Marga; Vilaseca, Maria Antonia; Balcells, Susana; Grinberg, Daniel

2006-01-01

Classical homocystinuria is due to cystathionine beta-synthase (CBS) deficiency. More than 130 mutations, which differ in prevalence and severity, have been described at the CBS gene. Mutation p.I278T is very prevalent, has been found in all European countries where it has been looked for with the exception of the Iberian peninsula, and is known to respond to vitamin B6. On the other hand, mutation p.T191M is prevalent in Spain and Portugal and does not respond to B6. We analysed 30 pedigrees from Spain, Portugal, Colombia and Argentina, segregating for homocystinuria. The p.T191M mutation was detected in patients from all four countries and was particularly prevalent in Colombia. The number of p.T191M alleles described in this study, together with those previously published, is 71. The prevalence of p.T191M among CBS mutant alleles in the different countries was: 0.75 in Colombia, 0.52 in Spain, 0.33 in Portugal, 0.25 in Venezuela, 0.20 in Argentina and 0.14 in Brazil. Haplotype analyses suggested a double origin for this mutation. No genotype-phenotype correlation other than the B6-nonresponsiveness could be established for the p.T191M mutation. Additionally, three new mutations, p.M173V, p.I429del and c.69_70+8del10, were found. The p.M173V was associated with a mild, B6-responsive, phenotype.

Human exposure to power frequency magnetic fields up to 7.6 mT: An integrated EEG/fMRI study.

Science.gov (United States)

Modolo, Julien; Thomas, Alex W; Legros, Alexandre

2017-09-01

We assessed the effects of power-line frequency (60 Hz in North America) magnetic fields (MF) in humans using simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI). Twenty-five participants were enrolled in a pseudo-double-blind experiment involving "real" or "sham" exposure to sinusoidal 60 Hz MF exposures delivered using the gradient coil of an MRI scanner following two conditions: (i) 10 s exposures at 3 mT (10 repetitions); (ii) 2 s exposures at 7.6 mT (100 repetitions). Occipital EEG spectral power was computed in the alpha range (8-12 Hz, reportedly the most sensitive to MF exposure in the literature) with/without exposure. Brain functional activation was studied using fMRI blood oxygen level-dependent (BOLD, inversely correlated with EEG alpha power) maps. No significant effects were detected on occipital EEG alpha power during or post-exposure for any exposure condition. Consistent with EEG results, no effects were observed on fMRI BOLD maps in any brain region. Our results suggest that acute exposure (2-10 s) to 60 Hz MF from 3 to 7.6 mT (30,000 to 76,000 times higher than average public exposure levels for 60 Hz MF) does not induce detectable changes in EEG or BOLD signals. Combined with previous findings in which effects were observed on the BOLD signal after 1 h exposure to 3 mT, 60 Hz MF, this suggests that MF exposure in the low mT range (<10 mT) might require prolonged durations of exposure to induce detectable effects. Bioelectromagnetics. 38:425-435, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Zīmēšana kā sešgadīga bērna iztēles veicinātāja pirmsskolas izglītības iestādē

OpenAIRE

Nazarova, Anželika

2014-01-01

Darba nosaukums: Zīmēšana kā sešgadīga bērna iztēles veicinātāja pirmsskolas izglītības iestādē Darba autore: Anželika Nazarova Zin. vadītāja: mag.paed.,Mg.phil. lekt. Ērika Lanka Darba apjoms: 67 lpp.,12 attēli, 3 shēmas, 4 tabulas, pielikumi, izmantoti 36 literatūras avoti Darba pētāmā problēma: Darbs veltīts problēmai, kā ar zīmēšanas palīdzību var veicināt iztēles attīstību. Pētījuma teorētiskā daļa balstās uz V.Hibneres, D.Lieģinieces., G.Svences, A.Ozoliņa Nucho, A.Vor...

Reproductive characteristics and population decline of four species of skate (Rajidae) off the eastern coast of Canada.

Science.gov (United States)

Mcphie, R P; Campana, S E

2009-07-01

Four of the most common species of skate (Rajidae) were studied off eastern Canada to determine if their reproductive characteristics were linked to their population trajectories. The fecundity of the winter skate Leucoraja ocellata, the little skate Leucoraja erinacea, the thorny skate Amblyraja radiata and the smooth skate Malacoraja senta averaged between 41 and 56 egg cases per year for each species. For all species but L. ocellata, males matured at larger sizes and at later ages than females. Theoretical rates of population increase for non-equilibrium populations of L. ocellata (c. 0.07), M. senta (c. 0.14) and L. erinacea and A. radiata (c. 0.20) were low compared to most fishes, indicating that north-west Atlantic skates are intrinsically unproductive, yet are theoretically capable of supporting low-level fisheries. Nevertheless, the results of 36 years of research surveys indicate that the abundance of mature L. ocellata, A. radiata and M. senta all decreased by >90% since 1970, indicating that past fishing mortality (both directed and undirected) has outstripped the net productivity of the skate populations on the eastern Scotian Shelf. The relationship between maximum age (t(max)) and age of maturity (t(mat)) was a better predictor of population growth rate than was body size, with the species exhibiting the highest ratios of t(mat) :t(max) (L. ocellata = 0.68, M. senta = 0.66) having the lowest predicted population growth rates. L. ocellata appears to have the lowest productivity and has experienced the greatest population decline, thus raising concerns over its future status.

Alterations in circulating T-cell lymphocyte populations in children with obstructive sleep apnea.

Science.gov (United States)

Tan, Hui-Leng; Gozal, David; Wang, Yang; Bandla, Hari P R; Bhattacharjee, Rakesh; Kulkarni, Richa; Kheirandish-Gozal, Leila

2013-06-01

Changes in lymphocyte phenotype and functionality have been described in adult patients with obstructive sleep apnea (OSA). We hypothesized that OSA is associated with T lymphocyte alterations in children, particularly in T regulatory lymphocytes (T regs), and aimed to characterize circulating T lymphocyte subsets in children with OSA. Cross-sectional. Kosair Children's Hospital (Louisville, KY, USA) and Comer Children's Hospital (Chicago, IL, USA). Consecutively recruited children being evaluated for habitual snoring. N/A. Overnight polysomnography (PSG) was performed and a fasting blood sample was obtained from the patients. Flow cytometry was performed on peripheral blood mononuclear cells stained for CD3, CD4, CD8, CD25, FOXP3, interleukin-4 (IL-4), interferon-γ (IFN-γ), and IL-17. Patients were divided into three groups based on their PSG: controls (apnea-hypopnea indices [AHI] hTST), moderate-severe OSA (AHI ≥ 5/h TST). The percentage of CD4+ and T reg lymphocytes differed across groups. Children with moderate-severe OSA had significantly reduced T reg than control children (median [interquartile range] 4.8 [3.8-5.7% CD4+] versus 7.8 [7.0-9.2% CD4+]; P < 0.001). There were also significant differences in the percentage of T helper 1 (Th1) lymphocytes and in Th1:Th2 ratios between groups. Children with moderate-severe OSA had increased Th1 cells (P = 0.001) and Th1:Th2 ratios (P = 0.0026) compared with children with mild OSA and control children. Associations between AHI and T reg (P = 0.0003; r = -0.46), CD4+ lymphocytes (P = 0.0047; r = -0.37), and Th1:Th2 ratios (P = 0.0009; r = 0.43) emerged. In addition, the percentage of T reg was inversely correlated with Th1:Th2 ratios (P = 0.029; r = -0.29). Pediatric OSA is associated with reduced T reg population and altered Th1:Th2 balance toward Th1 predominance, suggesting a shift to a proinflammatory state. The changes in lymphocytic phenotypes associated with OSA may contribute to the variance in systemic

A T cell-inducing influenza vaccine for the elderly: safety and immunogenicity of MVA-NP+M1 in adults aged over 50 years.

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Richard D Antrobus

Full Text Available Current influenza vaccines have reduced immunogenicity and are of uncertain efficacy in older adults. We assessed the safety and immunogenicity of MVA-NP+M1, a viral-vectored influenza vaccine designed to boost memory T cell responses, in a group of older adults.Thirty volunteers (aged 50-85 received a single intramuscular injection of MVA-NP+M1 at a dose of 1·5×10(8 plaque forming units (pfu. Safety and immunogenicity were assessed over a period of one year. The frequency of T cells specific for nucleoprotein (NP and matrix protein 1 (M1 was determined by interferon-gamma (IFN-γ ELISpot, and their phenotypic and functional properties were characterized by polychromatic flow cytometry. In a subset of M1-specific CD8(+ T cells, T cell receptor (TCR gene expression was evaluated using an unbiased molecular approach.Vaccination with MVA-NP+M1 was well tolerated. ELISpot responses were boosted significantly above baseline following vaccination. Increases were detected in both CD4(+ and CD8(+ T cell subsets. Clonality studies indicated that MVA-NP+M1 expanded pre-existing memory CD8(+ T cells, which displayed a predominant CD27(+CD45RO(+CD57(-CCR7(- phenotype both before and after vaccination.MVA-NP+M1 is safe and immunogenic in older adults. Unlike seasonal influenza vaccination, the immune responses generated by MVA-NP+M1 are similar between younger and older individuals. A T cell-inducing vaccine such as MVA-NP+M1 may therefore provide a way to circumvent the immunosenescence that impairs routine influenza vaccination.ClinicalTrials.gov NCT00942071.

{sup 99m}Tc-N.T.P. 15-5 imaging for the early and specific diagnosis of chondrosarcoma: proof of concept in rats

Energy Technology Data Exchange (ETDEWEB)

Miot-Noirault, E.; Vidal, A.; Rapp, M.; Madelmont, J.C.; Maublant, J.; Moins, N. [Institut National de la Sante et de la Recherche Medicale (INSERM), UMR 484, 63 - Clermont Ferrand (France); Redini, F.; Gouin, F.; Heymann, D. [Institut National de la Sante et de la Recherche Medicale (INSERM), ERI 7, EA 3822, 44 - Nantes (France)

2008-02-15

Aim. - The U.M.R. 484 I.N.S.E.R.M. develops a 'cartilage imaging strategy' with the {sup 99m}Tc-N.T.P. 15-5 tracer that selectively binds to cartilage proteoglycans, allowing a highly specific cartilage imaging. Since chondro-genic tumours are characterized by the presence of cartilaginous matrix, we hypothesized that the {sup 99m}Tc-N.T.P. 15-5 tracer would allow chondrosarcoma imaging, which is currently lacking in clinics. In the rat model of grade II para-tibial chondrosarcoma, we evaluated the relevance of {sup 99m}Tc-N.T.P. 15-5 imaging for an early and specific diagnosis of chondrosarcoma. Methods. - {sup 99m}Tc-N.T.P. 15-5 longitudinal imaging of animals was performed during two months after para-tibial ortho-topic tumour implantation in the right paw, the left being used as control. At regular intervals, animals were submitted to {sup 99m}Tc-M.D.P. bone imaging, the only examination used for SPECT diagnosis of chondrosarcoma in patients. Tumour volume was monitored for two months when the tumours became palpable, with the two perpendicular diameters measured. For both cartilage and bone imaging, the scans were considered positive when areas of tracer uptake were present at sites consistent with the sites of inoculation. For each animal, positive scans were analyzed at each stage using the semiquantitative method of the target to background ratio (T.B.R.), with the target R.O.I. being delineated over the tumour and background R.O.I. over vertebra and muscle. T.B.R. time-course was followed as a function of tumour growth.At study ending, each animal was sacrificed for histopathological control. Results. - {sup 99m}Tc-N.T.P. 15-5 scans were positive in 100% of the animals at very early stage (three days) after implantation, while no palpable nor measurable tumour could be assessed. Quantitative analysis of {sup 99m}Tc-N.T.P. 15-5 scans evidenced a significant uptake of the tracer at the implantation site at early stage. The time-course of T

CYP1B1 and myocilin gene mutations in Egyptian patients with ...

African Journals Online (AJOL)

Purpose: Primary congenital glaucoma (PCG) accounts for 26–29% of childhood blindness in Egypt. The identification of disease causing mutations has not been extensively investigated. We aimed to examine the frequency of CYP1B1 and MYOC mutations in PCG Egyptian patients, and study a possible ...

El rol del desempeño típico y máximo en selección de personal

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UTE CHRISTINE KLEHE

2007-01-01

Full Text Available Este artículo presenta una revisión de la investigación sobre la distinción entre desempeño típico y máximo. Comenzando con una distinción entre lo que los solicitantes pueden hacer (desempeño máximo y lo que ellos harán (desempeño típico, en términos de su desempeño probable en el puesto, demostraremos cómo tal distinción puede añadir información valiosa tanto para profesionales como para investigadores en selección de personal. Mientras que la distinción entre desempeño típico y máximo se ajusta bien a los actuales modelos de desempeño laboral y ha recibido considerable atención en contextos teóricos, suele ser pasada por alto tanto por investigadores como por profesionales en selección de personal. Los investigadores realizan extensos estudios de validación y las organizaciones hacen grandes inversiones financieras en la selección de nuevos empleados sin saber cuál de estos dos aspectos del rendimiento están prediciendo o están intentando predecir. Finalmente, propondremos algunas áreas para futura investigación en el estudio del desempeño típico y máximo.

Estadísticos poblacionales de Triatoma rubrovaria en condiciones de laboratorio Population statistics of Triatoma rubrovaria in laboratory

Directory of Open Access Journals (Sweden)

Elena B Oscherov

2005-04-01

Full Text Available OBJETIVO: Obtener parámetros poblacionales de T. rubrovaria a fin de caracterizar demográficamente a esta especie. MÉTODOS: La investigación se realizó entre octubre de 2000 y febrero de 2003 en el laboratorio de artrópodos, Corrientes, Argentina. Se conformaron cinco cohortes de 100 huevos cada una. Los insectos se alimentaron sobre gallina (Gallus domesticus. Las cohortes se controlaron semanalmente. La experiencia se llevó a cabo en condiciones controladas de temperatura (28±3ºC y humedad relativa del aire (63±10%. Se elaboraron tablas completas de vida y se obtuvieron estadísticos vitales. RESULTADOS: La mayor mortalidad se registró en ninfas de primero a cuarto estadio. A partir del quinto estadio el número de individuos decreció en forma constante. La expectativa de vida, después de superar las edades críticas, disminuyó en forma lineal. La supervivencia media de los adultos fue 50,2 semanas. La primera oviposición ocurrió a las 40,6 semanas. La fecundidad fue 859,6 huevos, con una media de 22,8 huevos. El período reproductivo fue de 37,7 semanas. El tiempo generacional fue de 55,3 semanas y la tasa neta de reproducción 133,7. La tasa intrínseca de incremento natural resultó 0,088. En una distribución estable de edades 25,3% correspondería al estado de huevo, 72,3% al estado ninfal y 2,4% al estado adulto. Los adultos contribuyeron con más del 70% al valor reproductivo total. CONCLUSIONES: Triatoma rubrovaria se caracterizó por una supervivencia prolongada como imago, una edad de la primera reproducción tardía y una tasa intrínseca de incremento natural baja.OBJECTIVE: To obtain T. rubrovaria population parameters in order to describe its demographic characteristics. METHODS: The study was carried out in the laboratory of Arthropods, Corrientes, Argentina, from October 2000 to February 2003. Eggs were grouped to form five 100-egg cohorts. Insects were fed on chickens (Gallus domesticus. The cohorts were

Quantitative and Qualitative Assessment of Pulmonary Emphysema with T2-Weighted PROPELLER MRI in a High-Risk Population Compared to Low-Dose CT.

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Meier-Schroers, Michael; Sprinkart, Alois Martin; Becker, Manuel; Homsi, Rami; Thomas, Daniel

2018-03-07

 To determine the suitability of T2-weighted PROPELLER MRI for the assessment of pulmonary emphysema.  60 participants in a lung cancer screening program (30 subjects with pulmonary emphysema, and 30 control subjects without emphysema) were included for this retrospective study. All subjects were examined with low-dose CT (LDCT) and MRI within the screening program. The use of a T2-weighted PROPELLER sequence for the assessment of emphysema was analyzed and correlated with the results of LDCT. The presence and the extent of pulmonary emphysema were first assessed qualitatively using a three-point score, and then quantitatively with a semi-automated software program to obtain emphysema indices.  All 30 cases with pulmonary emphysema were accurately detected by MRI. There were 3 cases with emphysema according to MRI without emphysematous changes on LDCT (false-positive results). The qualitative scores as well as the emphysema indices were significantly higher in the emphysema group compared to the control group for MRI and LDCT (p emphysema group and r = 0.668/p emphysema index: r = 0.960/p emphysema group and r = 0.746/p pulmonary emphysema may be assessed qualitatively and quantitatively by T2-weighted PROPELLER MRI with very good correlation to LDCT.   · T2-weighted PROPELLER MRI may be suitable for the assessment of pulmonary emphysema.. · There was significant correlation between MRI and LDCT regarding qualitative scores and quantitative emphysema indices in our study with correlation coefficients for different subgroups ranging from r = 0.668 to r = 0.960.. · T2-weighted PROPELLER MRI may have the potential to be used for follow-up examinations in patients with severe emphysema to avoid radiation exposure of repeated CTs.. · Meier-Schroers M, Sprinkart AM, Becker M et al. Quantitative and Qualitative Assessment of Pulmonary Emphysema with T2-Weighted PROPELLER MRI in a High-Risk Population Compared to Low-Dose CT

The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism.

Science.gov (United States)

Kleinberger, Gernot; Brendel, Matthias; Mracsko, Eva; Wefers, Benedikt; Groeneweg, Linda; Xiang, Xianyuan; Focke, Carola; Deußing, Maximilian; Suárez-Calvet, Marc; Mazaheri, Fargol; Parhizkar, Samira; Pettkus, Nadine; Wurst, Wolfgang; Feederle, Regina; Bartenstein, Peter; Mueggler, Thomas; Arzberger, Thomas; Knuesel, Irene; Rominger, Axel; Haass, Christian

2017-07-03

Genetic variants in the triggering receptor expressed on myeloid cells 2 (TREM2) increase the risk for several neurodegenerative diseases including Alzheimer's disease and frontotemporal dementia (FTD). Homozygous TREM2 missense mutations, such as p.T66M, lead to the FTD-like syndrome, but how they cause pathology is unknown. Using CRISPR/Cas9 genome editing, we generated a knock-in mouse model for the disease-associated Trem2 p.T66M mutation. Consistent with a loss-of-function mutation, we observe an intracellular accumulation of immature mutant Trem2 and reduced generation of soluble Trem2 similar to patients with the homozygous p.T66M mutation. Trem2 p.T66M knock-in mice show delayed resolution of inflammation upon in vivo lipopolysaccharide stimulation and cultured macrophages display significantly reduced phagocytic activity. Immunohistochemistry together with in vivo TSPO small animal positron emission tomography (μPET) demonstrates an age-dependent reduction in microglial activity. Surprisingly, perfusion magnetic resonance imaging and FDG-μPET imaging reveal a significant reduction in cerebral blood flow and brain glucose metabolism. Thus, we demonstrate that a TREM2 loss-of-function mutation causes brain-wide metabolic alterations pointing toward a possible function of microglia in regulating brain glucose metabolism. © 2017 The Authors.

Genetic variation underlying resistance to infectious hematopoietic necrosis virus in a steelhead trout (Oncorhynchus mykiss) population

Science.gov (United States)

Brieuc, Marine S. O.; Purcell, Maureen K.; Palmer, Alexander D.; Naish, Kerry A.

2015-01-01

Understanding the mechanisms of host resistance to pathogens will allow insights into the response of wild populations to the emergence of new pathogens. Infectious hematopoietic necrosis virus (IHNV) is endemic to the Pacific Northwest and infectious to Pacific salmon and trout (Oncorhynchus spp.). Emergence of the M genogroup of IHNV in steelhead trout O. mykiss in the coastal streams of Washington State, between 2007 and 2011, was geographically heterogeneous. Differences in host resistance due to genetic change were hypothesized to be a factor influencing the IHNV emergence patterns. For example, juvenile steelhead trout losses at the Quinault National Fish Hatchery (QNFH) were much lower than those at a nearby facility that cultures a stock originally derived from the same source population. Using a classical quantitative genetic approach, we determined the potential for the QNFH steelhead trout population to respond to selection caused by the pathogen, by estimating the heritability for 2 traits indicative of IHNV resistance, mortality (h2 = 0.377 (0.226 - 0.550)) and days to death (h2 = 0.093 (0.018 - 0.203)). These results confirm that there is a genetic basis for resistance and that this population has the potential to adapt to IHNV. Additionally, genetic correlation between days to death and fish length suggests a correlated response in these traits to selection. Reduction of genetic variation, as well as the presence or absence of resistant alleles, could affect the ability of populations to adapt to the pathogen. Identification of the genetic basis for IHNV resistance could allow the assessment of the susceptibility of other steelhead populations.

Laser-radiation therapy for T2N0M0 laryngeal-glottic cancer

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Suzuki, Gen; Hayabuchi, Naofumi; Toda, Yukihiro; Suefuji, Hiroaki; Ogo, Etuyo; Nakajima, Tadashi [Kurume Univ., Fukuoka (Japan). School of Medicine

2002-03-01

The purpose of this study was to evaluate the laser-radiation combined therapy for T2N0M0 laryngeal-glottic cancer in order to preserve the larynx. The subjects consisted of 52 patients with T2N0M0 laryngeal-glottic cancer treated with laser-radiation combined therapy between 1980 and 1999. Patients ranged in age from 40-88 years, with a median of 70 years, and included 51 men and one woman. During this period, treatment was administered with different radiation devices ({sup 60}Co or 4 MV-X ray), and 40-72 Gy (median, 60Gy) of radiation therapy were administered. Tumor and treatment characteristics were correlated with local control at a median follow-up of 61 months (range 12-210 months). Concurrent chemotherapy was administered to 32 patients; 29 were treated with 5-FU and vitamin A (FAR), and 3 were treated with low-dose CDDP. Post treatment vocal function was examined in 37 patients. The voice was evaluated in terms of four parameters: maximum phonation time (MPT), mean air flow rate during phonation over a comfortable duration (MFR), fundamental frequency range of phonation (F0 range), and sound pressure level range of phonation (SPL range). The five-year cause-specific-survival rate was 98.0%, and the local control rate was 91.8%. Three of 4 patients who had locally relapsed were administered total laryngectomy as salvage therapy. One patient was administered the tracheostomy for late complication. The actuarial laryngeal preservation rate was 92.3%. We did not find any significant relationship between local relapse and extent of disease, subglottic extension, or anterior commissure involvement. Concurrent chemotherapy was not a significant prognostic factor. Laser debulking followed by radiation therapy did not change the voice significantly except the F0 range. We conclude that the laser-radiation combined therapy for T2N0M0 laryngeal-glottic cancer was effective therapy for not only preservation of the voice but also for vocal function. (author)

Laser-radiation therapy for T2N0M0 laryngeal-glottic cancer

International Nuclear Information System (INIS)

Suzuki, Gen; Hayabuchi, Naofumi; Toda, Yukihiro; Suefuji, Hiroaki; Ogo, Etuyo; Nakajima, Tadashi

2002-01-01

The purpose of this study was to evaluate the laser-radiation combined therapy for T2N0M0 laryngeal-glottic cancer in order to preserve the larynx. The subjects consisted of 52 patients with T2N0M0 laryngeal-glottic cancer treated with laser-radiation combined therapy between 1980 and 1999. Patients ranged in age from 40-88 years, with a median of 70 years, and included 51 men and one woman. During this period, treatment was administered with different radiation devices ( 60 Co or 4 MV-X ray), and 40-72 Gy (median, 60Gy) of radiation therapy were administered. Tumor and treatment characteristics were correlated with local control at a median follow-up of 61 months (range 12-210 months). Concurrent chemotherapy was administered to 32 patients; 29 were treated with 5-FU and vitamin A (FAR), and 3 were treated with low-dose CDDP. Post treatment vocal function was examined in 37 patients. The voice was evaluated in terms of four parameters: maximum phonation time (MPT), mean air flow rate during phonation over a comfortable duration (MFR), fundamental frequency range of phonation (F0 range), and sound pressure level range of phonation (SPL range). The five-year cause-specific-survival rate was 98.0%, and the local control rate was 91.8%. Three of 4 patients who had locally relapsed were administered total laryngectomy as salvage therapy. One patient was administered the tracheostomy for late complication. The actuarial laryngeal preservation rate was 92.3%. We did not find any significant relationship between local relapse and extent of disease, subglottic extension, or anterior commissure involvement. Concurrent chemotherapy was not a significant prognostic factor. Laser debulking followed by radiation therapy did not change the voice significantly except the F0 range. We conclude that the laser-radiation combined therapy for T2N0M0 laryngeal-glottic cancer was effective therapy for not only preservation of the voice but also for vocal function. (author)

Clinical evaluation of a novel population-based regression analysis for detecting glaucomatous visual field progression.

Science.gov (United States)

Kovalska, M P; Bürki, E; Schoetzau, A; Orguel, S F; Orguel, S; Grieshaber, M C

2011-04-01

The distinction of real progression from test variability in visual field (VF) series may be based on clinical judgment, on trend analysis based on follow-up of test parameters over time, or on identification of a significant change related to the mean of baseline exams (event analysis). The aim of this study was to compare a new population-based method (Octopus field analysis, OFA) with classic regression analyses and clinical judgment for detecting glaucomatous VF changes. 240 VF series of 240 patients with at least 9 consecutive examinations available were included into this study. They were independently classified by two experienced investigators. The results of such a classification served as a reference for comparison for the following statistical tests: (a) t-test global, (b) r-test global, (c) regression analysis of 10 VF clusters and (d) point-wise linear regression analysis. 32.5 % of the VF series were classified as progressive by the investigators. The sensitivity and specificity were 89.7 % and 92.0 % for r-test, and 73.1 % and 93.8 % for the t-test, respectively. In the point-wise linear regression analysis, the specificity was comparable (89.5 % versus 92 %), but the sensitivity was clearly lower than in the r-test (22.4 % versus 89.7 %) at a significance level of p = 0.01. A regression analysis for the 10 VF clusters showed a markedly higher sensitivity for the r-test (37.7 %) than the t-test (14.1 %) at a similar specificity (88.3 % versus 93.8 %) for a significant trend (p = 0.005). In regard to the cluster distribution, the paracentral clusters and the superior nasal hemifield progressed most frequently. The population-based regression analysis seems to be superior to the trend analysis in detecting VF progression in glaucoma, and may eliminate the drawbacks of the event analysis. Further, it may assist the clinician in the evaluation of VF series and may allow better visualization of the correlation between function and structure owing to VF

Recours aux médicaments et aux consultations psychologiques chez les Canadiens atteints d'un trouble de l'humeur et/ou d'anxiété

Directory of Open Access Journals (Sweden)

Siobhan O'Donnell

2017-01-01

Full Text Available Introduction : L'étude décrit le recours aux médicaments sur ordonnance et aux consultations psychologiques au cours des 12 derniers mois chez les Canadiens adultes ayant déclaré avoir reçu un diagnostic de trouble de l'humeur et/ou d'anxiété, les caractéristiques sociodémographiques et cliniques associées à ce recours et les raisons invoquées pour ne pas y recourir. Méthodologie : L'Enquête sur les personnes ayant une maladie chronique au Canada - Composante sur les troubles de l'humeur et d'anxiété de 2014 a été utilisée. L'échantillon de l'étude (n = 2 916 a été divisé en quatre sous-groupes de traitement : (1 prend des médicaments seulement; (2 a reçu des consultations psychologiques seulement; (3 prend des médicaments et a reçu des consultations psychologiques; ou (4 n'a eu recours à aucun de ces deux traitements. Nous avons combiné les trois premiers sous-groupes et effectué des analyses descriptives et de régression logistique multivariée pour comparer ceux qui prenaient des médicaments et/ou avaient reçu des consultations psychologiques par rapport à ceux n'ayant pas eu recours à ces deux traitements. Nous avons pondéré toutes les estimations afin que les données soient représentatives de la population canadienne adulte vivant en logement privé dans l'une des 10 provinces et ayant déclaré avoir reçu un diagnostic de troubles de l'humeur et/ou d'anxiété. Résultats : La majorité (81,8 % des Canadiens adultes ayant déclaré avoir reçu un diagnostic de trouble de l'humeur et/ou d'anxiété ont indiqué prendre des médicaments et/ou avoir reçu des consultations psychologiques (47,6 % prenaient des médicaments seulement, 6,9 % avaient reçu des consultations psychologiques seulement et 27,3 % avaient eu recours aux deux modalités de traitement. Après ajustement des caractéristiques individuelles, le recours aux médicaments et/ou aux consultations psychologiques était significativement

Variation of sigma-hole magnitude with M valence electron population in MX(n)Y(4-n) molecules (n = 1-4; M = C, Si, Ge; X, Y = F, Cl, Br).

Science.gov (United States)

McDowell, Sean A C; Joseph, Jerelle A

2014-01-14

Sigma holes are described as electron-deficient regions on atoms, particularly along the extension of covalent bonds, due to non-uniform electron density distribution on the surface of these atoms. A computational study of MX(n)Y(4-n) molecules (n = 1-4; M = C, Si, Ge; X, Y = F, Cl, Br) was undertaken and it is shown that the relative sigma hole potentials on M due to X-M and Y-M can be adequately explained in terms of the variation in the valence electron population of the central M atom. A model is proposed for the depletion of the M valence electron population which explains the trends in sigma hole strengths, especially those that cannot be accounted for solely on the basis of relative electronegativities.

Population-level impact of white-nose syndrome on the endangered Indiana bat

Science.gov (United States)

Thogmartin, Wayne E.; King, R. Andrew; McKann, Patrick C.; Szymanski, Jennifer A.; Pruitt, Lori

2012-01-01

Establishing status and trend for an endangered species is critical to recovery, especially when it is faced with a nascent extinction agent. We calculated, with hierarchical log-linear change-point models, hibernaculum-level population trends between 1983 and 2009 for the endangered Indiana bat (Myotis sodalis) now subjected to the fast-spreading fungal disease white-nose syndrome. We combined trends from 222 wintering populations before and after onset of the disease to determine trend for clusters of interacting wintering populations, recovery units, and the species. Before onset of the disease, a west-to-east gradient in trends existed, with westernmost populations declining and easternmost populations increasing in abundance. The species as a whole, however, was stationary between 1983 and 2005 (-0.5% mean annual change; 95% confidence interval [CI] = -2.8, +1.8%). Estimated mean population size in 2009 was 377,124 bats (195,398-957,348), with large variance apparently caused by white-nose syndrome. With the onset of white-nose syndrome (2006-2009), the species exhibited a 10.3% annual decline (95% CI = -21.1, +2.0%). White-nose syndrome is having an appreciable influence on the status and trends of Indiana bat populations, stalling and in some cases reversing population gains made in recent years.

The Hubble Space Telescope UV Legacy Survey of Galactic globular clusters - XIV. Multiple stellar populations within M 15 and their radial distribution

Science.gov (United States)

Nardiello, D.; Milone, A. P.; Piotto, G.; Anderson, J.; Bedin, L. R.; Bellini, A.; Cassisi, S.; Libralato, M.; Marino, A. F.

2018-06-01

In the context of the Hubble Space Telescope UV Survey of Galactic globular clusters (GCs), we derived high-precision, multi-band photometry to investigate the multiple stellar populations in the massive and metal-poor GC M 15. By creating for red-giant branch (RGB) stars of the cluster a `chromosome map', which is a pseudo two-colour diagram made with appropriate combination of F275W, F336W, F438W, and F814W magnitudes, we revealed colour spreads around two of the three already known stellar populations. These spreads cannot be produced by photometric errors alone and could hide the existence of (two) additional populations. This discovery increases the complexity of the multiple-population phenomenon in M 15. Our analysis shows that M 15 exhibits a faint sub-giant branch (SGB), which is also detected in colour-magnitude diagrams (CMDs) made with optical magnitudes only. This poorly populated SGB includes about 5 per cent of the total number of SGB stars and evolves into a red RGB in the mF336W versus mF336W - mF814W CMD, suggesting that M 15 belongs to the class of Type II GCs. We measured the relative number of stars in each population at various radial distances from the cluster centre, showing that all of these populations share the same radial distribution within statistic uncertainties. These new findings are discussed in the context of the formation and evolution scenarios of the multiple populations.

Arco Vara asutaja müüs tüki firmast Toomas Toolile / Andres Eilart

Index Scriptorium Estoniae

Eilart, Andres

2008-01-01

Arti Arakase ettevõte Greatway müüs 5,25% Arco Vara aktsiatest ärimees Toomas Toolile. A. Arakas on koos teiste ärimeestega asutanud uue kinnisvarafirma EfTEN Capital. Ärimees T. Toolist. Lisa: Ühiseid projekte pole seni olnud

Data of evolutionary structure change: 1M3DI-1T60W [Confc[Archive

Lifescience Database Archive (English)

Full Text Available tryIDChain>1M3DI APLPM--MPVAE ...> -- EEE> ATOM 14546 CA ALA I 86 32.542 22.976 78.017 1.00...ain>1T60W EPMPMSMAPITG > EEE> IECHG-RGTCN >EEEE - EEEE.../entryIDChain> IECNGARGTCH >EEEEGGGEEEE>

Majanduspoliitilised võimalused sündimuse ja naiste tööturukäitumise mõjutamiseks Eestis / Marge Birk, Andres Võrk

Index Scriptorium Estoniae

Birk, Marge

2003-01-01

Ülevaade Eestis toimunud sündimuse ja naiste tööjõus osalemise määra arengutest viimasel aastakümnel; Euroopa riikide kogemustest tööturul osalemise, peretoetuste ja sündimuse seoste kohta; majanduspoliitilistest võimalustest sündimuse mõjutamiseks Eestis. Graafikud. Tabel

Readiness of Adults to Learn Using E-Learning, M-Learning and T-Learning Technologies

Science.gov (United States)

Vilkonis, Rytis; Bakanoviene, Tatjana; Turskiene, Sigita

2013-01-01

The article presents results of the empirical research revealing readiness of adults to participate in the lifelong learning process using e-learning, m-learning and t-learning technologies. The research has been carried out in the framework of the international project eBig3 aiming at development a new distance learning platform blending virtual…

Evaluation of some irradiated populations of sunflower (Helianthus Annus) in M4 and M5 generation. Vol. 4

International Nuclear Information System (INIS)

Atia, Z.M.A.

1996-01-01

Seeds of the M 3 generation of irradiated and irradiated populations were divided into three parts, the first part was sown on 28/8/1993, the second on 1/1/1994, and the third part on 1/5/1995 to evaluate seed yield and other characters for the three dates of planting. Results showed significant increase in head and seed weight, head and stem diameter and seed index in the first and third time of planting. On the contrast shelling percentage, and plant height decreased significantly in the two times of planting. In the second time of planting 1/1/1995, head and seed weight, shelling percentage, sterility zone, plant and stem diameter decreased significantly in the irradiated populations as compared with control especially using doses of gamma rays (120 and 160 Gy). Number of rows/head was not significantly affected for the three dates of planting. 2 tabs

The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo

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Jehn-Chuan Lee

2016-08-01

Full Text Available Oral squamous cell carcinoma (OSCC is a common malignancy with a growing worldwide incidence and prevalence. The N-myc downstream regulated gene (NDRG family of NDRG1, 2, 3, and mammary serine protease inhibitor (Maspin gene are well-known modulators in the neoplasia process. Current research has considered iron chelators as new anti-cancer agents; however, the anticancer activities of iron chelators and their target genes in OSCC have not been well investigated. We showed that iron chelators (Dp44mT, desferrioxamine (DFO, and deferasirox all significantly inhibit SAS cell growth. Flow cytometry further indicated that Dp44mT inhibition of SAS cells growth was partly due to induction of G1 cell cycle arrest. Iron chelators enhanced expressions of NDRG1 and NDRG3 while repressing cyclin D1 expression in OSCC cells. The in vivo antitumor effect on OSCC and safety of Dp44mT were further confirmed through a xenograft animal model. The Dp44mT treatment also increased Maspin protein levels in SAS and OECM-1 cells. NDRG3 knockdown enhanced the growth of OECM-1 cells in vitro and in vivo. Our results indicated that NDRG3 is a tumor suppressor gene in OSCC cells, and Dp44mT could be a promising therapeutic agent for OSCC treatment.

Ethnobotanique des plantes médicinales anti hémorroïdaires des ...

African Journals Online (AJOL)

SARAH

2015-12-31

Dec 31, 2015 ... but est de valoriser la médecine à base des plantes dans le traitement des pathologies ... médicinales anti hémorroïdaires et le coût réduit des recettes serait pour les populations démunies, ...... l'Afrique, Unesco/Aetfat/Unso.

Trichoderma asperellum strain T34 controls Fusarium wilt disease in tomato plants in soilless culture through competition for iron.

Science.gov (United States)

Segarra, Guillem; Casanova, Eva; Avilés, Manuel; Trillas, Isabel

2010-01-01

Trichoderma asperellum strain T34 has been reported to control the disease caused by Fusarium oxysporum f.sp. lycopersici (Fol) on tomato plants. To study the importance of iron concentration in the growth media for the activity and competitiveness of T34 and the pathogen, we tested four iron concentrations in the nutrient solution [1, 10, 100, and 1000 microM provided as EDTA/Fe(III)] in a biological control experiment with T34 and Fol in tomato plants. The reduction of the Fusarium-infected shoot by T34 was only significant at 10 microM Fe. We hypothesized that Fe competition is one of the key factors in the biocontrol activity exerted by T34 against Fol, as an increase in Fe concentration over 10 microM would lead to the suppression of T34 siderophore synthesis and thus inhibition of Fe competition with Fol. T34 significantly reduced the populations of Fol at all the doses of Fe assayed. In contrast, Fol enhanced the populations of T34 at 1 and 10 microM Fe. Nevertheless, several plant physiological parameters like net CO(2) assimilation (A), stomatal conductance (g(s)), relative quantum efficiency of PSII (Phi(PSII)), and efficiency of excitation energy capture by open PSII reactive centers (Fv'/Fm') demonstrated the protection against Fol damage by treatment with T34 at 100 microM Fe. The first physiological parameter affected by the disease progression was g(s). Plant dry weight was decreased by Fe toxicity at 100 and 1,000 microM. T34-treated plants had significantly greater heights and dry weights than control plants at 1,000 microM Fe, even though T34 did not reduce the Fe content in leaves or stems. Furthermore, T34 enhanced plant height even at the optimal Fe concentration (10 microM) compared to control plants. In conclusion, T. asperellum strain T34 protected tomato plants from both biotic (Fusarium wilt disease) and abiotic stress [Fe(III) toxic effects].

Konservierung der männlichen Fertilität - Klinische und experimentelle Methoden

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Gassei K

2009-01-01

Full Text Available Gängige Therapieansätze zur Behandlung von Krebserkrankungen können die Spermatogenese auf unterschiedlichsten Wegen stören und zu temporärer Azoospermie oder Sterilität führen. Die Unfruchtbarkeit ist eine ernstzunehmende Langzeitnebenwirkung, die die Lebensqualität des Patienten nachhaltig einschränken kann. Bei Kinderwunsch nach erfolgreicher Krebstherapie steht erwachsenen Patienten heute eine Anzahl an assistierten Reproduktionstechniken (ART wie In-vitro-Fertilisation (IVF oder Intrazytoplasmatischer Spermieninjektion (ICSI zur Verfügung, die mithilfe von kryokonservierten Spermien durchgeführt werden können. Diese Methoden können jedoch die natürliche Fertilität des Patienten nicht wiederherstellen und sind für präpubertäre Krebspatienten ungeeignet, da diese vor Beginn der Chemo- oder Radiotherapie keine Samenprobe liefern können. Die Transplantation von testikulären Stammzellen bietet daher einen möglichen Therapieansatz zur Behandlung von Unfruchtbarkeit. Methoden zur Isolierung, Konservierung, Transplantation und In-vitro-Maturierung dieser Zellen befinden sich zur Zeit in der Entwicklung. Der vorliegende Beitrag diskutiert etablierte Methoden zur Fertilitätskonservierung bei Männern und gibt darüber hinaus einen Überblick über die neuesten Forschungsergebnisse zur Keimzelltransplantation und extrakorporalen Generierung von Keimzellen.

Characterization of the expanded T cell population in infectious mononucleosis: apoptosis, expression of apoptosis-related genes, and Epstein–Barr virus (EBV) status

Science.gov (United States)

Verbeke, C S; Wenthe, U; Bergler, W F; Zentgraf, H

2000-01-01

Infectious mononucleosis (IM), a manifestation of primary infection with EBV, is characterized by a massive expansion of the T cell population. In this study we examined this expanded T cell population regarding its EBV status, its proliferative and apoptotic activity, and its expression of apoptosis-related genes. Whereas previous studies were performed on ex vivo cultures or on peripheral blood, our investigations included in vivo analysis of IM tonsillectomy specimens (14 cases) by in situ hybridization for viral RNA (EBERs) combined with immunohistochemistry (IHC; CD3, CD45RO, CD20, CD79a, Ki-67, Bcl-2, Bax, Fas, FasL) and the TUNEL method. Of the EBER+ cells 50–70% showed expression of the B cell markers CD20/CD79a. The remainder of the EBER+ cells expressed neither B nor T cell antigens. No co-expression of EBERs and T cell antigens was detected in any of the specimens. In accordance with a high rate of apoptosis (up to 2·37%) within the expanded T cell population, Bcl-2 expression was drastically reduced and FasL expression remarkably increased. The levels of Bax and Fas expression showed no or moderate up-regulation. In conclusion, the massive expansion of IM T cells is not caused by EBV infection of these cells but merely represents an intense immune reaction. Through altered expression of Bcl-2/Bax and Fas/FasL, the activated T cells are subject to enhanced apoptosis while residing within the lymphoid tissue, which eventually allows the efficient silencing of this potentially damaging T cell response. PMID:10792379

Dağıtım Kanallarında Çatışma Nasıl Önlenebilir?/Prevention of Conflict in Distribution Channels

OpenAIRE

Güreş, Nuriye

2014-01-01

Müşteri memnuniyetinin önem kazandığı günümüzde, müşterinin talep ettiği ürün ve hizmetleri doğru yerde, doğru zamanda ve doğru fiyattan sunmak işletmeler açısından büyük önem arz etmektedir. Bu noktada, ürün ve hizmetlerin müşterilere ulaştırılmasında etkin rol oynayan dağıtım kanallarının önemi ortaya çıkmaktadır. Dolayısıyla bu çalışmada, müşterilere istedikleri ürün ve hizmetleri ulaştırmada çok önemli yeri olan dağıtım kanalları üzerinde durularak, dağıtım kanallarında çeşitli sebeplerl...

The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes.

Directory of Open Access Journals (Sweden)

Maria Górska

2010-06-01

Full Text Available Type 1 diabetes mellitus (T1DM is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha, activatory molecules (OX40, 4-1BB and elements of cytotoxicity (granzyme B, perforin 1 were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.

The Stellar Populations Inside Expanding HI Shells in the Spiral Galaxy M33

Science.gov (United States)

Walterbos, Rene

1997-07-01

Because of its vigorous star formation activity, favorable inclination, and relative proximity, M33 is an ideal laboratory for the study of expanding HI shells in spiral galaxies. Theoretical models show that the energy deposited into the ISM by high mass stars in OB associations is capable of creating HI superbubbles. However, sparse observational evidence exists to test these models in detail. One essential ingredient of such a test is an improved census of stellar populations inside expanding HI shells. Using multi-color archival HST images of M33, we will {1} verify that association ages are consistent with dynamical ages of related shells and with ages from model predictions for bubbles of matching size and kinematics; {2} Constrain the IMF for each association by combining integrated ground-based HAlpha fluxes with the population age, present day mass function, and luminosity function derived from WFPC2 data; {3} Use this information to infer which fraction of the integrated stellar mechanical luminosity is transferred to a shell over its lifetime. Ground-based observations of associations inside expanding shells lack the UV-sensitivity and spatial resolution to adequately address these issues. Our sample of expanding neutral shells in M33 was selected using a new automated method for analysis of HI datacubes. From this robust catalog we have identified more than 30 HI supershells in M33 already imaged with WFPC2 in suitable broadband filters {F160BW, F170W, F336W, F439W, F555W, and F814W}.

Coding Instructions, Worksheets, and Keypunch Sheets for M.E.T.R.O.-APEX Simulation.

Science.gov (United States)

Michigan Univ., Ann Arbor. Environmental Simulation Lab.

Compiled in this resource are coding instructions, worksheets, and keypunch sheets for use in the M.E.T.R.O.-APEX simulation, described in detail in documents ED 064 530 through ED 064 550. Air Pollution Exercise (APEX) is a computerized college and professional level "real world" simulation of a community with urban and rural problems, industrial…

Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

DEFF Research Database (Denmark)

Chen, C Y; Gherzi, R; Andersen, Jens S.

2000-01-01

Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' un...

First-Trimester Maternal Alcohol Consumption and the Risk of Infant Oral Clefts in Norway: A Population-based Case-Control Study

OpenAIRE

DeRoo, Lisa A.; Wilcox, Allen J.; Drevon, Christian A.; Lie, Rolv Terje

2008-01-01

Although alcohol is a recognized teratogen, evidence is limited on alcohol intake and oral cleft risk. The authors examined the association between maternal alcohol consumption and oral clefts in a national, population-based case-control study of infants born in 1996–2001 in Norway. Participants were 377 infants with cleft lip with or without cleft palate, 196 with cleft palate only, and 763 controls. Mothers reported first-trimester alcohol consumption in self-administered questionnaires com...

A comparative study of radiographic landmarks of T.M.J. by various techniques

International Nuclear Information System (INIS)

Lee, You Dong; Ahn, Hyung Kyu

1974-01-01

The author has studied roentgenographic images of temporomandibular articulation using various conventional roentgenographies. The roentgenographic images have been obtained by application of the contrast media on the glenoid fossa and condylar head in human dry skull. Comparing the various roentgenograms by Modified Transcranial projection, A-P T.M. articulation projection, Reverse Town projection, Mayer projection, and Bregma-Menton projection; The author has drawn following results. 1. The sharp radiographic details were obtained by all technics used except the Bregma-Menton projection which seemed to be impractical to the study of T.M.J. because of the shortened image of condylar head. 2. The best image of the condyle-fossa relationship was appeared by Modified Transcranial projection and better image w as acquired by Orbito-Ramus projection, but there were all inferior in Reverse Towne projection, Mayer projection, and B regma-Menton projection. 3. In all of the above techniques, the radiograhpic images of condylar head were clear and appeared to be the convex type in Modified Transcranial projection, the angled type in Orbito-Ramus and Reverse Towne projection, the flat type in Mayer projection, and the distorted angled type in Bregma-Menton projection. 4. The radiographic image of condylar head was shortened in Bregma-Menton projection only and was magnified somewhat in other projections.

A comparative study of radiographic landmarks of T.M.J. by various techniques

Energy Technology Data Exchange (ETDEWEB)

Lee, You Dong; Ahn, Hyung Kyu [College of Dentistry, Seoul National University, Seoul (Korea, Republic of)

1974-11-15

The author has studied roentgenographic images of temporomandibular articulation using various conventional roentgenographies. The roentgenographic images have been obtained by application of the contrast media on the glenoid fossa and condylar head in human dry skull. Comparing the various roentgenograms by Modified Transcranial projection, A-P T.M. articulation projection, Reverse Town projection, Mayer projection, and Bregma-Menton projection; The author has drawn following results. 1. The sharp radiographic details were obtained by all technics used except the Bregma-Menton projection which seemed to be impractical to the study of T.M.J. because of the shortened image of condylar head. 2. The best image of the condyle-fossa relationship was appeared by Modified Transcranial projection and better image w as acquired by Orbito-Ramus projection, but there were all inferior in Reverse Towne projection, Mayer projection, and B regma-Menton projection. 3. In all of the above techniques, the radiograhpic images of condylar head were clear and appeared to be the convex type in Modified Transcranial projection, the angled type in Orbito-Ramus and Reverse Towne projection, the flat type in Mayer projection, and the distorted angled type in Bregma-Menton projection. 4. The radiographic image of condylar head was shortened in Bregma-Menton projection only and was magnified somewhat in other projections.

Interleukin-9 receptor α chain mRNA formation in CD8+ T cells producing anti-human immunodeficiency virus type 1 substance(s)

International Nuclear Information System (INIS)

Hossain, M.M.; Tsuchie, H.; Detorio, M.A.; Shirono, H.; Hara, C.; Nishimoto, A.; Saji, A.; Koga, J.; Takata, N.; Maniar, J.K.; Saple, D.G.; Taniguchi, K.; Kageyama, S.; Ichimura, H.; Kurimura, T.

1998-01-01

A search for gene(s) associated with anti-human immunodeficiency virus type 1 (HIV-l) activity of CD8 + T cells was attempted using molecular cloning and the relation between the anti-HIV activity of CD8 + T cells and the interleukin-9 receptor a chain (IL-9R-α) mRNA expression from the cDNA clones obtained was examined. The anti-HIV-l activity of CD8 + T cell culture supernatants was assessed by measuring the level of HIV-l replication in a CD4 + T cell line transfected with an infectious HIV-l DNA clone. IL-9R-a mRNA was assayed by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 5 cases showing high level of anti-HIV-l activity (more than 80% suppression of HIV-l replication), the mRNA was detected in 4 cases. Of 10 cases showing low level of anti-HIV-l activity (less than 80% suppression of HIV-l replication), the mRNA was detected in one case. Soluble recombinant human IL-9 receptor (rhIL-9sR) did not suppress HIV-l replication at a concentration of 1 μg/ml. These data suggest that the IL-9R-a mRNA formation in CD8 + T cells may correlate with and play some role in the anti-HIV-l activity of CD8+ T cells from HIV-l-infected individuals. Key words: CD8+ T cells; anti-HIV-l activity; cytokines; interleukin-9 receptor (authors)

EGFR T790M mutation after chemotherapy for small cell lung cancer transformation of EGFR-positive non-small cell lung cancer

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Tomoaki Sonoda

Full Text Available In non-small cell lung cancer (NSCLC with an epidermal growth factor receptor (EGFR mutation, 50%–65% of cases acquire resistance after treatment with EGFR-tyrosine kinase inhibitors (EGFR-TKIs because of an EGFR T790M point mutation and 3%–14% of these cases transformed to small cell lung cancer (SCLC. Generally, the EGFR T790M secondary mutation develops with ongoing ATP competitive inhibition. We present a case of a 76-year-old woman with lung adenocarcinoma harboring an EGFR-L858R mutation who received first-line gefitinib and developed SCLC transformation. She was administered several chemotherapy agents, including a platinum doublet. The primary lesion that showed SCLC transformation had reconverted to adenocarcinoma with EGFR L858R and T790M mutations at the time of a second re-biopsy. Therefore, she was administered osimertinib, which resulted in clinical remission. This case suggested that serial biopsies are necessary even after SCLC transformation. Keywords: NSCLC, EGFR mutation, SCLC transformation, T790M, Osimertinib

Theoretical descriptions of novel triplet germylenes M1-Ge-M2-M3 (M1 = H, Li, Na, K; M2 = Be, Mg, Ca; M3 = H, F, Cl, Br).

Science.gov (United States)

Kassaee, Mohamad Zaman; Ashenagar, Samaneh

2018-02-06

In a quest to identify new ground-state triplet germylenes, the stabilities (singlet-triplet energy differences, ΔE S-T ) of 96 singlet (s) and triplet (t) M 1 -Ge-M 2 -M 3 species were compared and contrasted at the B3LYP/6-311++G**, QCISD(T)/6-311++G**, and CCSD(T)/6-311++G** levels of theory (M 1  = H, Li, Na, K; M 2  = Be, Mg, Ca; M 3  = H, F, Cl, Br). Interestingly, F-substituent triplet germylenes (M 3  = F) appear to be more stable and linear than the corresponding Cl- or Br-substituent triplet germylenes (M 3  = Cl or Br). Triplets with M 1  = K (i.e., the K-Ge-M 2 -M 3 series) seem to be more stable than the corresponding triplets with M 1  = H, Li, or Na. This can be attributed to the higher electropositivity of potassium. Triplet species with M 3  = Cl behave similarly to those with M 3  = Br. Conversely, triplets with M 3  = H show similar stabilities and linearities to those with M 3  = F. Singlet species of formulae K-Ge-Ca-Cl and K-Ge-Ca-Br form unexpected cyclic structures. Finally, the triplet germylenes M 1 -Ge-M 2 -M 3 become more stable as the electropositivities of the α-substituents (M 1 and M 2 ) and the electronegativity of the β-substituent (M 3 ) increase.

[Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats].

Science.gov (United States)

Gao, Yu-Hai; Zhou, Yan-Feng; Li, Shao-Feng; Li, Wen-Yuan; Xi, Hui-Rong; Yang, Fang-Fang; Chen, Ke-Ming

2017-12-25

To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats. Thirty SD rats weighted(110±10) and aged 1 month were randomly divided into control group and electromagnetic field group, 15 in each group. Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week, and observed food-intake. Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body, and detected bone density of femur and vertebral body. Osteocalcin and tartrate-resistant acid phosphatase 5b were detected by ELSA; weighted liver, kidney and uterus to calculate purtenance index, then detected pathologic results by HE. Compared with control group, there was no significant change in weight every week, food-intake every day; no obvious change of bone density of whole body at 2 and 4 weeks, however bone density of whole body, bone density of excised femur and vertebra were increased at 6 weeks. Expression of OC was increased, and TRACP 5b expression was decreased. No change of HE has been observed in liver, kidney and uterus and organic index. 50 Hz 1.8 mT sinusoidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs, to improve peak bone density of young rats, in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.

Genetic variations in a PTEN/AKT/mTOR axis and prostate cancer risk in a Chinese population.

Directory of Open Access Journals (Sweden)

Jiawei Chen

Full Text Available Genetic variations in a PTEN/AKT/mTOR signaling axis may influence cellular functions including cell growth, proliferation and apoptosis, and then increase the individual's risk of cancer. Accordingly, we explore the association between single nucleotide polymorphisms (SNPs of these genes and prostate cancer (PCa in our Chinese population.Subjects were recruited from 666 PCa patients and 708 cancer-free controls, and eight SNPs in the PTEN/AKT/mTOR axis were determined by the TaqMan assay. Odds ratios (OR and 95% confidence intervals (95% CI were evaluated by logistic regression.We observed significant associations between PCa risk and mTOR rs2295080 [P = 0.027, OR = 0.85, 95%CI = 0.74-0.98], and AKT2 rs7254617 (P = 0.003, OR = 1.35, 95%CI = 1.11-1.64. When estimated these two SNPs together, the combined genotypes with 2-4 risk alleles (rs2295080 T and rs7254617 A alleles were associated with an increased risk of PCa compared with 0-1 risk alleles, which was more pronounced among subgroups of age >71 years, smokers, drinkers and no family history of cancer. Results of stratified analyses by cliniopathological parameters revealed that the frequencies of the combined genotypes with 2-4 risk alleles in advanced stage were significantly higher than in localized stage (P = 0.022, but there was no significant association in Gleason score and PSA level.Our results indicate, for the first time that the two variants in AKT2 and mTOR, particularly the joint genotypes with 2-4 risk alleles may influence PCa susceptibility and progression in Chinese, and the association appeared to be more strong in the subgroup of smokers and drinkers.

MEASUREMENT OF THE MASS AND STELLAR POPULATION DISTRIBUTION IN M82 WITH THE LBT

Energy Technology Data Exchange (ETDEWEB)

Greco, Johnny P.; Martini, Paul; Thompson, Todd A., E-mail: greco.40@buckeyemail.osu.edu [Department of Astronomy, Ohio State University, Columbus, OH 43210 (United States)

2012-09-20

We present a K-band spectroscopic study of the stellar and gas kinematics, mass distribution, and stellar populations of the archetypical starburst galaxy M82. Our results are based on a single spectrum at a position angle of 67.{sup 0}5 through the K-band nucleus. We used the {sup 12}CO stellar absorption band head at 2.29 {mu}m (CO{sub 2.29}) to measure the rotation curve out to nearly 4 kpc radius on both the eastern and western sides of the galaxy. Our data show that the rotation curve is flat from 1 to 4 kpc. This stands in sharp contrast to some previous studies, which have interpreted H I and CO emission-line position-velocity diagrams as evidence for a declining rotation curve. The kinematics of the Br{gamma}, H{sub 2}, and He I emission lines are consistent with, although characterized by slightly higher velocities than, the stellar kinematics. We derived M82's mass distribution from our stellar kinematic measurements and estimate that its total dynamical mass is {approx}10{sup 10} M{sub Sun }. We measured the equivalent width of CO{sub 2.29} (W{sub 2.29}) as a function of distance from the center of the galaxy to investigate the spatial extent of the red supergiant (RSG) population. The variation in W{sub 2.29} with radius clearly shows that RSGs dominate the light inside 500 pc radius. M82's superwind is likely launched from this region, where we estimate that the enclosed mass is {approx}<2 Multiplication-Sign 10{sup 9} M{sub Sun }.

Depressive symptoms in seniors’ population in Maringá, PR Prevalência da sintomatologia depressiva em idosos em uma área de saúde da família no município de Maringá, Estado do Paraná

Directory of Open Access Journals (Sweden)

Mauro Porcu

2002-04-01

Full Text Available This paper aims to verify the prevalence of depressive symptoms in the seniors’ population, over 60 years, in a PSF restricted area, district of Maringá - PR. An oblique study was accomplished using Beck’s Inventory for Depression. From a population of 451 individuals, a sample of 123 people were analyzed (27,3% of the total. Results were the following: 1 average age ≅ 69 ± 8 years; 2 dysphoria: 15.1% for men and 17.2% for women (p=0,7590; depression: 34% for men and 37.7% for women (p=0,6942; 3 the depression prevalence was 4.8 times higher than the Brazilian population and 2.1 times of the American seniors. It was concluded that the depressive symptoms were very high in the seniors’ sample studies of a PSF areaVerificar a prevalência da sintomatologia depressiva na população de idosos com idade superior a 60 anos, numa área adstrita do PSF, no município de Maringá, Estado do Paraná. Foi realizado um estudo transversal, utilizando o Inventário para Depressão de Beck. A população estudada foi de 451 indivíduos, da qual foi utilizada uma amostra de 123 pessoas correspondendo a 27,3% do total. Obtiveram-se os seguintes resultados: 1 idade média ≅ 69 ± 8 anos; 2 disforia: 15,1% dos homens e 17,2% das mulheres (p = 0,7590; depressão: 34% dos homens e 37,7% das mulheres (p = 0,6942; 3 a prevalência de “depressão” foi 4,8 vezes maior que a população brasileira e 2,1 vezes a dos idosos americanos. Assim, a sintomatologia depressiva foi altamente prevalente na amostra de idosos estudada, de uma área de PSF

Population Structure among Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Colombia

Science.gov (United States)

Realpe, Teresa; Correa, Nidia; Rozo, Juan Carlos; Ferro, Beatriz Elena; Gomez, Verónica; Zapata, Elsa; Ribon, Wellman; Puerto, Gloria; Castro, Claudia; Nieto, Luisa María; Diaz, Maria Lilia; Rivera, Oriana; Couvin, David; Rastogi, Nalin; Arbelaez, Maria Patricia; Robledo, Jaime

2014-01-01

Background Phylogeographic composition of M. tuberculosis populations reveals associations between lineages and human populations that might have implications for the development of strategies to control the disease. In Latin America, lineage 4 or the Euro-American, is predominant with considerable variations among and within countries. In Colombia, although few studies from specific localities have revealed differences in M. tuberculosis populations, there are still areas of the country where this information is lacking, as is a comparison of Colombian isolates with those from the rest of the world. Principal Findings A total of 414 M. tuberculosis isolates from adult pulmonary tuberculosis cases from three Colombian states were studied. Isolates were genotyped using IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, and 24-locus Mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTRs). SIT42 (LAM9) and SIT62 (H1) represented 53.3% of isolates, followed by 8.21% SIT50 (H3), 5.07% SIT53 (T1), and 3.14% SIT727 (H1). Composite spoligotyping and 24-locus MIRU- VNTR minimum spanning tree analysis suggest a recent expansion of SIT42 and SIT62 evolved originally from SIT53 (T1). The proportion of Haarlem sublineage (44.3%) was significantly higher than that in neighboring countries. Associations were found between M. tuberculosis MDR and SIT45 (H1), as well as HIV-positive serology with SIT727 (H1) and SIT53 (T1). Conclusions This study showed the population structure of M. tuberculosis in several regions from Colombia with a dominance of the LAM and Haarlem sublineages, particularly in two major urban settings (Medellín and Cali). Dominant spoligotypes were LAM9 (SIT 42) and Haarlem (SIT62). The proportion of the Haarlem sublineage was higher in Colombia compared to that in neighboring countries, suggesting particular conditions of co-evolution with the corresponding human population that favor the success of this

The dynamics of locked mode development in the T-11M tokamak

International Nuclear Information System (INIS)

Belov, A.M.

2002-01-01

Recent results of locked mode (LM) development studies in the T-11M tokamak are submitted. A particular interest in this type of plasma MHD-activity arises from the circumstance that an appropriate plasma perturbation is quasi-stationary and potentially could destroy plasma confinement, if it exceeds the same critical level. There are evidences to believe that LM amplitude approaches this critical level in the stage preceding a major disruption, resulting in the reduction of the magnetic shear in the plasma center, which finally initiates the disruption. (author)

Association of the TCF7L2 rs12255372 (G/T variant with type 2 diabetes mellitus in an Iranian population

Directory of Open Access Journals (Sweden)

Faranak Mahmoudi Alami

2012-01-01

Full Text Available In various populations worldwide, common variants of the TCF7L2 (Transcription factor 7-like 2 gene are associated with the risk of type 2 diabetes mellitus (T2DM. The aim was to investigate the association between rs12255372 (G/T polymorphism in the TCF7L2 gene and T2DM in an Iranian population. 236 unrelated patients with T2DM, and 255 normoglycemic controls without diabetes were studied. The PCR-RFLP method was used for genotyping rs12255372 (G/T polymorphism, and the SPSS version 18.0 for Windows for statistical analysis. The minor T allele of TCF7L2 rs12255372 was found to significantly increase the risk of T2DM, with an allelic odds ratio (OR of 1.458 (95% CI 1.108-1.918, p = 0.007. A significant difference in TT genotype was observed between T2DM patients and normoglycemic controls (OR 2.038, 95% CI 1.147-3.623; p = 0.014. On assuming dominant and recessive models, ORs of 1.52 [95% CI (1.05-2.21 p = 0.026] and 1.74 [95% CI (1.01-3.00 p = 0.043] were obtained, respectively, thereby implying that the co-dominant model would best fit the susceptible gene effect. This study further confirms the TCF7L2 gene as enhancing susceptibility to the development of T2DM.

Mixed Phenotype Acute Leukemia with Two Immunophenotypically Distinct B and T Blasts Populations, Double Chromosome and Complex Karyotype: Report of an Unusual Case

Directory of Open Access Journals (Sweden)

Samah A. Kohla

2015-01-01

Full Text Available Mixed phenotype acute leukemia (MPAL is considered as a rare type of leukemia with an incidence of less than 4% of all acute leukemia based on the most recent 2008 WHO classification. Common subtypes are the B/myeloid and T/myeloid; B/T and trilineage MPAL being extremely rare. We present a case of a male in his 20s, whose peripheral blood smears showed 34% blast cells and bone marrow with 70% blasts. Immunophenotyping by multiparametric flow cytometry showed two populations of blasts, the major one with B-lineage and the minor one with T-lineage. Conventional karyotyping revealed complex karyotype with the presence of double Philadelphia chromosome ( Ph + . BCR/ABL1 rearrangement was confirmed by fluorescent in situ hybridization (FISH analysis. The BCR/ABL1 ES probe on interphase cells indicated pl90 minor m-BCR/ABL fusion in 46% and a second abnormal clone with double Ph + in 16% of the cells analyzed confirmed by reverse transcription-PCR (RT-PCR. The case was diagnosed as MPAL with double Philadelphia chromosome Ph + . The patient was treated with dasatinib, four cycle hyper CVAD/methotrexate cytarabin protocol, and allogeneic transplant. He is still alive in complete hematological, cytogenetic, and molecular remission. Mixed phenotype B/T acute leukemia is an extremely rare disease, particularly those with double Philadelphia chromosomes and clinically presents challenges in diagnosis and treatment.

Kansai dialekts Japānas populārajā kultūrā – anime un manga.

OpenAIRE

Rubene, Gunta

2014-01-01

Bakalaura darbs „Kansai dialekts Japānas populārajā kultūrā - anime un manga” ir veltīts Kansai dialekta lomas izpētei šajos populārās kultūras medijos. Pētījums atspoguļo stereotipus, kas mūsdienu japāņu sabiedrībā saistās ar Kansai dialektu un tā tipisku runātāju. Tiek apskatīti anime un mangas personāži kā šo stereotipu nesēji. Darbs sastāv no četrām daļām. Pirmā daļa sniedz teorētisko bāzi, kas ir nepieciešama, lai izprastu stereotipu par Kansai dialektu rašanās vēsturiskos un sociālo...

John Berry : rahvuse kaardile mängimine toob tõsiseid tagajärgi / John Berry ; interv. Raivo Vetik

Index Scriptorium Estoniae

Berry, John

2007-01-01

Kanada emeriitprofessor rahvuslikust turvatundest, sobivast nimetusest mitte-eestlaste kohta, pronkssõduri teisaldamisest, Venemaaga suhtlemisest, ajaloosündmuste erinevast mõtestamisest, sh. ka Kanadas; kakskeelsusest, muukeelse eestimeelsuse võimalikkusest. Professori sõnul saab ajaloosündmusi õpetada nii, et näidatakse nende erinevaid tõlgendusi, ta soovitab õpetada mõlemaid käsitlusi ja lasta õpilastel mõtiskleda erinevuste üle

Measurement of the top-quark mass and the t anti tZ cross section in ATLAS. The t anti t dilepton way

International Nuclear Information System (INIS)

Wong, Kaven Henry Yau

2015-05-01

A measurement of the top-quark mass and the t anti tZ cross section is performed using 4.6 fb -1 and 20.3 fb -1 of data from proton-proton collisions collected with the ATLAS detector at the LHC. The top-quark mass is measured in the t anti t eμ channel using the mean value of the m T2 variable with the calibration curve method to obtain the first top-quark mass measurement in the dilepton channel using the ATLAS detector: m top =175.2±1.6 (stat.)± 3.1(syst.) GeV. Improvements in the computation of the systematic uncertainty, the measurement method and the inclusion of the t anti t ee and μμ channels result in a significant increase in the precision of the measurement, leading to a measured top-quark mass of m top =173.7±0.8(stat.)±1.8 (syst.) GeV. The use of the m T2 perp variable is also studied, providing an additional mass measurement with similar uncertainty: m top =173.3±0.7(stat.)±1.7 (syst.) GeV. A t anti tZ cross-section measurement is performed in the t anti tZ→4l channel using a likelihood fit to five signal regions and one ZZ control region used to determine the normalization of the main background from data. Seven events are selected in data and, from the result of the likelihood fit, a t anti tZ cross section of σ t anti tZ =0.32 +0.18 -0.14 (stat.) +0.12 -0.05 (syst.) pb is measured, which is compatible with the Standard Model prediction for proton-proton collisions at 8 TeV.

Duševní stav málo a pravidelně tělesně aktivních žen ve druhém trimestru těhotenství The state of mind of less physically active and regularly physically active women in the second trimester of their pregnancies

Directory of Open Access Journals (Sweden)

Damir Karpljuk

2008-04-01

Full Text Available V okamžiku, kdy žena zjistí, že je těhotná, se její život v mnoha ohledech změní, protože se začne přizpůsobovat dítěti ve svém těle. Přeje si dobře se cítit, v bezpečí dosáhnout stanoveného termínu a porodit zdravé dítě. Průzkum jsme prováděli u 163 těhotných žen na konci druhého trimestru těhotenství. Článek představuje vztah mezi sportovní aktivitou a duševním stavem během těhotenství. Srovnávali jsme dvě skupiny žen – ženy s pravidelnou tělesnou aktivitou (PTA a ženy s nízkou úrovní tělesné aktivity (NTA – a zkoumali vztah mezi tělesnou aktivitou a duševním stavem. První část výzkumu se zaměřovala na tělesnou aktivitu, a to zvláště na její četnost, formu a typ. Skupina PTA sestávala z 69 těhotných žen, které se pravidelně věnují organizovaným sportovním aktivitám ve sportovních střediscích nebo které jsou tělesně aktivní neorganizovaně třikrát až čtyřikrát za týden. V potaz jsme brali aktivity trvající 30 a více minut. Skupina NTA sestávala z 94 těhotných žen, které se tělesným aktivitám věnují pouze příležitostně nebo které nejsou tělesně aktivní. Druhá část výzkumu týkajícího se duševního stavu těhotných žen obsahovala 45 položek, které se týkaly jejich duševního stavu. U každé položky ženy určovaly svůj duševní stav na pětistupňové škále (nikdy, málo, občas, často, skoro pořád. Z celkového počtu mělo 20 položek prevalenci pozitivního duševního stavu a 25 prevalenci negativního duševního stavu. U obou skupin jsme analyzovali duševní stav a vztahy mezi pravidelnou sportovní aktivitou a nízkou sportovní aktivitou a duševním stavem v druhém trimestru, kdy jsou těhotné ženy nejčastěji a nejsnáze tělesně aktivní. Pomocí faktorové analýzy jsme prokázali, že u skupiny PTA je prvním eliminovaným faktorem faktor spojený s pozitivními emocemi, což vysv

Hollywoody tähtede kõrval on müügil suur kinnistu / Kaire Talviste

Index Scriptorium Estoniae

Talviste, Kaire, 1979-

2008-01-01

Müüki on pandud Hollywoodi tähtede (1923. a. rajatud ja ajalooliseks mälestiseks kuulutatud) kõrval asuv kunagi Howard Hughesile kuulunud kinnistu, et rajada sinna kuni viis villat. Los Angelese linnavõimud on ehituse vastu ja koguvad raha, et eraomandis olev krunt ära osta

Mouse lysozyme-M knockout mice reveal how the self-determinant hierarchy shapes the T cell repertoire against this circulating self antigen in wild-type mice

NARCIS (Netherlands)

Sinha, Pratima; Chi, Howard H.; Kim, Hong R.; Clausen, Björn E.; Pederson, Brian; Sercarz, Eli E.; Forster, Irmgard; Moudgil, Kamal D.

2004-01-01

We have studied T cell tolerance to defined determinants within ML-M using wild-type (WT; ML-M+/+) and LysMcre (ML-M-/-) C3H (H-2(k)) mice to determine the relative contribution of ML-M-derived epitopes vs those from other self Ags in selection of the ML-M-specific T cell repertoire. ML-M was

Association of TCF7L2 gene polymorphisms with T2DM in the population of Hyderabad, India.

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Kommoju Uma Jyothi

Full Text Available We attempt to evaluate the nature of association of TCF7L2 gene variants with T2DM, for the first time in the population of Hyderabad, which is considered to be diabetic capital of India. It is a case-control study of the three SNPs of TCF7L2, rs7903146, rs12255372 and rs11196205, genotyped on Sequenom Massarray platform, in a sample of 758 patients and 621 controls. The risk allele frequency of the three SNPs was found to be significantly higher in the T2DM cases than controls, implicating susceptibility for diabetes (p<0.01. The greatest risk of developing the disease was conferred by rs7903146. Further, the logistic regression of genotypes of each SNP under log additive model, and the haplotypes constituted by at least one of the three risk alleles also show significantly greater risk of developing T2DM when compared to the wild type haplotype. Further, BMI and WHR emerge as significant covariates with confounding effects. The strong association of the TCF7L2 SNPs with T2DM is consistent with the findings among other Indian and Non-Indian populations, suggesting universal phenomena of its association across ethnic groups globally, both within and outside the Indian subcontinent, albeit the functional relevance of these SNPs needs yet to be established.

Triiodothyronine increases mRNA and protein leptin levels in short time in 3T3-L1 adipocytes by PI3K pathway activation.

Directory of Open Access Journals (Sweden)

Miriane de Oliveira

Full Text Available The present study aimed to examine the effects of thyroid hormone (TH, more precisely triiodothyronine (T3, on the modulation of leptin mRNA expression and the involvement of the phosphatidyl inositol 3 kinase (PI3K signaling pathway in adipocytes, 3T3-L1, cell culture. We examined the involvement of this pathway in mediating TH effects by treating 3T3-L1 adipocytes with physiological (P=10nM or supraphysiological (SI=100 nM T3 dose during one hour (short time, in the absence or the presence of PI3K inhibitor (LY294002. The absence of any treatment was considered the control group (C. RT-qPCR was used for mRNA expression analyzes. For data analyzes ANOVA complemented with Tukey's test was used at 5% significance. T3 increased leptin mRNA expression in P (2.26 ± 0.36, p 0.001. These results demonstrate that the activation of the PI3K signaling pathway has a role in TH-mediated direct and indirect leptin gene expression in 3T3-L1 adipocytes.

Conditional cancer-specific mortality in T4, N1, or M1 prostate cancer: implications for long-term prognosis

International Nuclear Information System (INIS)

Muralidhar, Vinayak; Mahal, Brandon A.; Nguyen, Paul L.

2015-01-01

The risk of prostate cancer-specific mortality (PCSM) following a diagnosis of prostate cancer may improve after patients have survived a number of years after diagnosis. We sought to determine long-term conditional PCSM for patients with stage T4, N1, or M1 prostate cancer. We identified 66,817 patients diagnosed with stage IV (T4N0M0, N1M0, or M1) prostate cancer between 1973 and 2011 using the Surveillance, Epidemiology, and End Results (SEER) database. Conditional five-year PCSM was evaluated for each group of patients at 5, 10, and 15 years of survival according to the Fine & Gray model for competing risks after adjusting for tumor grade, age, income level, and marital status. Race-stratified analyses were also performed. There were 13,345 patients with T4 disease, 12,450 patients with N1 disease, and 41,022 patients with M1 disease. Median follow-up among survivors in the three groups was 123 months (range: 0–382 months), 61 months (range: 0–410 months), and 30 months (range: 0–370 months), respectively. Conditional PCSM improved in all three groups over time. Among patients with T4 disease, 5-year PCSM improved from 13.9 % at diagnosis to 11.2, 8.1, and 6.5 % conditioned on 5, 10, or 15 years of survival, respectively (p < 0.001 in all cases). In patients with N1 disease, 5-year PCSM increased within the first five years and decreased thereafter, from 18.9 % at diagnosis to 21.4 % (p < 0.001), 17.6 % (p = 0.055), and 13.8 % (p < 0.001), respectively. In patients with metastatic disease, 5-year PCSM improved from 57.2 % at diagnosis to 41.1, 28.8, and 20.8 %, respectively (p < 0.001). White race was associated with a greater increase in conditional survival compared to non-white race among those with T4 or N1 disease. While patients with T4, N1, or M1 prostate cancer are never “cured,” their odds of cancer-specific survival increase substantially after they have survived for 5 or more years. Physicians who take care of patients with prostate cancer

Properties of M1-M2-Si-Al-O-N glasses (M1 = La or Nd, M2 = Y or Er)

Energy Technology Data Exchange (ETDEWEB)

Pomeroy, M.J.; Nestor, E.; Hampshire, S. [Limerick Univ. (Ireland). Materials and Surface Science Inst.; Ramesh, R. [Littelfuse Ireland, Dundalk, Co. Louth (Ireland)

2002-07-01

Mixed lanthanide cation oxynitride glasses have been prepared in the M1 - M2 - Si-Al-O-N systems where M1 = La or Nd and M2 = Y or Er. The densities ({rho}), Young's moduli (E), microhardnesses (H{sub v}), glass transition temperatures (T{sub g}), dilatometric softening temperatures (T{sub dil}) and coefficients of thermal expansion (CTE) of 13 glasses were determined. The molar volume values (MV) calculated from density data, E, H{sub v}, T{sub g}, T{sub dil} and CTE values were all found to vary linearly with the effective cation field strength arising from the M1 and M2 modifier cations. Least squares intercept and slope values are presented which correlate each property to effective cation field strength together with error values which arise from glass and specimen preparation and measurement inconsistencies. These linear correlations clearly indicate that the overall glass structure remains the same for each of the thirteen glasses with only the modifier cation(s) having any influence. This influence appears to be a cross-linking effect, the strength of which increases as the effective cation field strength of the M1, M2 modifiers increases. (orig.)

High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells

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Chang, L.; Liu, Y.Y.; Zhu, B.; Li, Y.; Hua, H.; Wang, Y.H.; Zhang, J.; Jiang, Z.; Wang, Z.R. [Sichuan University, Chengdu (China). West China Medical Center. Health Ministry Key Lab. of Chronobiology], e-mail: wangzhengrong@126.com

2009-10-15

Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to {sup 60}Co-{gamma}-rays. NIH 3T3 cells were treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with {sup 6}0Co-{gamma}-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection: pcDNA3.1-mPer2 3.7% vs pcDNA3.1 11.3% and control 8.2%), more proliferation and clonogenic survival (TPA: 121.7 {+-} 6.51 vs 66.0 {+-} 3.51 and 67.7 {+-} 7.37; transfection: 121.7 {+-} 6.50 vs 65.3 {+-} 3.51 and 69.0 {+-} 4.58) both when treated with TPA and transfected with mPer2. RT-PCR analysis showed an increased expression of bax, bcl-2, p53, cmyc, mre11, and nbs1, and an increased proportionality of bcl-2/bax in the irradiated cells at peak mPer2 expression compared with cells at trough mPer2 expression and control cells. However, no significant difference in rad50 expression was observed among the three groups of cells. Immunohistochemistry also showed increased protein levels of P53, BAX and proliferating cell nuclear antigen in irradiated cells with peak mPer2 levels. Thus, high expression of the circadian gene mPer2 may reduce the radiosensitivity of NIH 3T3 cells. For this effect, mPer2 may directly or indirectly regulate the expressions of cell proliferation- and apoptosis-related genes and DNA repair-related genes. (author)

High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells

International Nuclear Information System (INIS)

Chang, L.; Liu, Y.Y.; Zhu, B.; Li, Y.; Hua, H.; Wang, Y.H.; Zhang, J.; Jiang, Z.; Wang, Z.R.

2009-01-01

Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to 60 Co-γ-rays. NIH 3T3 cells were treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 6 0Co-γ-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection: pcDNA3.1-mPer2 3.7% vs pcDNA3.1 11.3% and control 8.2%), more proliferation and clonogenic survival (TPA: 121.7 ± 6.51 vs 66.0 ± 3.51 and 67.7 ± 7.37; transfection: 121.7 ± 6.50 vs 65.3 ± 3.51 and 69.0 ± 4.58) both when treated with TPA and transfected with mPer2. RT-PCR analysis showed an increased expression of bax, bcl-2, p53, cmyc, mre11, and nbs1, and an increased proportionality of bcl-2/bax in the irradiated cells at peak mPer2 expression compared with cells at trough mPer2 expression and control cells. However, no significant difference in rad50 expression was observed among the three groups of cells. Immunohistochemistry also showed increased protein levels of P53, BAX and proliferating cell nuclear antigen in irradiated cells with peak mPer2 levels. Thus, high expression of the circadian gene mPer2 may reduce the radiosensitivity of NIH 3T3 cells. For this effect, mPer2 may directly or indirectly regulate the expressions of cell proliferation- and apoptosis-related genes and DNA repair-related genes. (author)

Characterization of CD8+ T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL

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Daphné Benteyn

2013-01-01

Full Text Available Treatment of melanoma patients with mRNA electroporated dendritic cells (TriMixDC-MEL stimulates T-cell responses against the presented tumor-associated antigens (TAAs. In the current clinical trials, melanoma patients with systemic metastases are treated, requiring priming and/or expansion of preexisting TAA-specific T cells that are able to migrate to both the skin and internal organs. We monitored the presence of TAA-specific CD8+ T cells infiltrating the skin at sites of intradermal TriMixDC-MEL injection (SKILs and within the circulation of melanoma patients treated in two clinical trials. In 10 out of fourteen (71% patients screened, CD8+ T cells recognizing any of the four TAA presented by TriMixDC-MEL cellular vaccine were found in both compartments. In total, 30 TAA-specific T-cell responses were detected among the SKILs and 29 among peripheral blood T cells, of which 24 in common. A detailed characterization of the antigen specificity of CD8+ T-cell populations in four patients indicates that the majority of the epitopes detected were only recognized by CD8+ T cells derived from either skin biopsies or peripheral blood, indicating that some compartmentalization occurs after TriMix-DC therapy. To conclude, functional TAA-specific CD8+ T cells distribute both to the skin and peripheral blood of patients after TriMixDC-MEL therapy.

Characterization of CD8+ T-cell responses in the peripheral blood and skin injection sites of melanoma patients treated with mRNA electroporated autologous dendritic cells (TriMixDC-MEL).

Science.gov (United States)

Benteyn, Daphné; Van Nuffel, An M T; Wilgenhof, Sofie; Corthals, Jurgen; Heirman, Carlo; Neyns, Bart; Thielemans, Kris; Bonehill, Aude

2013-01-01

Treatment of melanoma patients with mRNA electroporated dendritic cells (TriMixDC-MEL) stimulates T-cell responses against the presented tumor-associated antigens (TAAs). In the current clinical trials, melanoma patients with systemic metastases are treated, requiring priming and/or expansion of preexisting TAA-specific T cells that are able to migrate to both the skin and internal organs. We monitored the presence of TAA-specific CD8(+) T cells infiltrating the skin at sites of intradermal TriMixDC-MEL injection (SKILs) and within the circulation of melanoma patients treated in two clinical trials. In 10 out of fourteen (71%) patients screened, CD8(+) T cells recognizing any of the four TAA presented by TriMixDC-MEL cellular vaccine were found in both compartments. In total, 30 TAA-specific T-cell responses were detected among the SKILs and 29 among peripheral blood T cells, of which 24 in common. A detailed characterization of the antigen specificity of CD8(+) T-cell populations in four patients indicates that the majority of the epitopes detected were only recognized by CD8(+) T cells derived from either skin biopsies or peripheral blood, indicating that some compartmentalization occurs after TriMix-DC therapy. To conclude, functional TAA-specific CD8(+) T cells distribute both to the skin and peripheral blood of patients after TriMixDC-MEL therapy.

Evaluation of an mRNA lipofection procedure for human dendritic cells and induction of cytotoxic T lymphocytes against enhanced green fluorescence protein.