WorldWideScience

Sample records for system gene libraries

  1. A rapid pathway toward a superb gene delivery system: programming structural and functional diversity into a supramolecular nanoparticle library.

    Science.gov (United States)

    Wang, Hao; Liu, Kan; Chen, Kuan-Ju; Lu, Yujie; Wang, Shutao; Lin, Wei-Yu; Guo, Feng; Kamei, Ken-ichiro; Chen, Yi-Chun; Ohashi, Minori; Wang, Mingwei; Garcia, Mitch André; Zhao, Xing-Zhong; Shen, Clifton K-F; Tseng, Hsian-Rong

    2010-10-26

    Nanoparticles are regarded as promising transfection reagents for effective and safe delivery of nucleic acids into a specific type of cells or tissues providing an alternative manipulation/therapy strategy to viral gene delivery. However, the current process of searching novel delivery materials is limited due to conventional low-throughput and time-consuming multistep synthetic approaches. Additionally, conventional approaches are frequently accompanied with unpredictability and continual optimization refinements, impeding flexible generation of material diversity creating a major obstacle to achieving high transfection performance. Here we have demonstrated a rapid developmental pathway toward highly efficient gene delivery systems by leveraging the powers of a supramolecular synthetic approach and a custom-designed digital microreactor. Using the digital microreactor, broad structural/functional diversity can be programmed into a library of DNA-encapsulated supramolecular nanoparticles (DNA⊂SNPs) by systematically altering the mixing ratios of molecular building blocks and a DNA plasmid. In vitro transfection studies with DNA⊂SNPs library identified the DNA⊂SNPs with the highest gene transfection efficiency, which can be attributed to cooperative effects of structures and surface chemistry of DNA⊂SNPs. We envision such a rapid developmental pathway can be adopted for generating nanoparticle-based vectors for delivery of a variety of loads.

  2. LIBRARY MANAGEMNT INFORMATION SYSTEM

    Directory of Open Access Journals (Sweden)

    Magnolia Tilca

    2013-06-01

    Full Text Available The focus of any educational institution is the content and services of the university library. The mission of the library is to obtain, organize, preserve and update the information with the greatest possible accuracy, minimum effort and time. This requires automation of the library’s operations. This paper presents a software application for managing the activity of the territorial "Vasile Goldiş" West University library. The application is developed using Visual Basic for Application programming language and using the database management system Microsoft Access 2010. The goal of this application is to optimize the inner workings of local library and to meet the requests of the institution and of the readers.

  3. Library Online Systems.

    Science.gov (United States)

    Folda, Linda; And Others

    1989-01-01

    Issues related to library online systems are discussed in six articles. Topics covered include staff education through vendor demonstrations, evaluation of online public access catalogs, the impact of integrated online systems on cataloging operations, the merits of smart and dumb barcodes, and points to consider in planning for the next online…

  4. SECURING DIGITIZED LIBRARY CIRCULATORY SYSTEM

    African Journals Online (AJOL)

    user

    The widespread application of the developed system on smart library circulation .... database management system; [9] through securing .... system running on a Windows 8 Operating system .... mini library for their support, advice and unlimited.

  5. Automated Library System Specifications.

    Science.gov (United States)

    1986-06-01

    Battelle Software Products), TOMAS (Carlyle Systems, Inc), ULYSIS/84 (Universal Library Systems), Unicorn Collection Management System (Sirsi Corp...STOR{ AGE CAPA3ILITY Dependent on mixc of processors an~d dikdrives. ’KxIUI OFREORS Depends upon mix of application. programs, and includes...BASIC -- - -Ultiniate, Prime, DEC VAXv PZRIHERIALS SUPPORTED Wyse, Viewpoints, Inter.ec lig;htpens, HP Ili~htpens, C-Itoh STO AGE CAPABILITY 66 MB to

  6. Library Information-Processing System

    Science.gov (United States)

    1985-01-01

    System works with Library of Congress MARC II format. System composed of subsystems that provide wide range of library informationprocessing capabilities. Format is American National Standards Institute (ANSI) format for machine-readable bibliographic data. Adaptable to any medium-to-large library.

  7. Library Systems: FY 2013 Public Libraries Survey (Administrative Entity)

    Data.gov (United States)

    Institute of Museum and Library Services — Find key information on library systems around the United States.These data include imputed values for libraries that did not submit information in the FY 2013 data...

  8. Library Systems: FY 2012 Public Libraries Survey (Administrative Entity)

    Data.gov (United States)

    Institute of Museum and Library Services — Find key information on library systems around the United States.These data include imputed values for libraries that did not submit information in the FY 2012 data...

  9. Library Systems: FY 2014 Public Libraries Survey (Administrative Entity Data)

    Data.gov (United States)

    Institute of Museum and Library Services — Find key information on library systems around the United States.These data include imputed values for libraries that did not submit information in the FY 2014 data...

  10. Library system of Italy

    Directory of Open Access Journals (Sweden)

    Nataša Gerbec

    2003-01-01

    Full Text Available In the European extent, Italy is the cradle of libraries and library sciences. In the past, Italian national public libraries played an important role through their vast book treasury. But only during the last thirty years have public libraries been developed following the Anglo-American public library model. Italy does not have any uniform or general legislation concerning libraries. On the state level, this area is regulated by some separate acts, while on the regional level there is a collection of various acts and regulations. Libraries are not strictly divided into general categories. It is required that the professionals engaged in Italian libraries should have secondary or university education. The level of their professional tasks depends on the type of library and its capacity. The competency for the development in the field of librarianship is assigned to The Ministry of Cultural and Environment Heritage as well as to its subordinate institutions (Central Institute for the Union catalogue of Italian Libraries and for Bibliographic Information, Central Institute for Book Pathology, Observatory for International Libraries Programmes.

  11. Our Stories Transforming Our Libraries: The York County Library System

    Directory of Open Access Journals (Sweden)

    Mina Edmondson

    2016-11-01

    Full Text Available These narratives chronicle the authors’ journeys to collaborate and discover the transformative impact that stories have on library culture and library staff. This study describes a research collaboration between York County Libraries and Penn State York. In Phase I, we collected stories from library staff as the library system was being challenged to reimage public libraries for the future. The major themes and types of organizational stories identified in the initial narrative project were presented during a county-wide all-staff in-service training. The library District Consultant (first author and the Penn State professor (second author then facilitated a workshop designed to lead staff in their exploration of these topics and generate a written record of their storytelling/discussions. This data became the basis for Phase II of the project and allowed the system to strategically assess its evolving culture and identity.

  12. Invenio as a library system

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    As a join2 partner, DESY library uses Invenio already for it's publication database and institutional repository. The next logical step is to also migrate the library catalogue from the currently used Aleph system to Invenio. Starting out with a short introduction of how to migrate Aleph. This includes the migration of bibliographic data as well as holdings but also movement data, current loans etc. The talk also outlines some of the new additions required to run Invenio as an ILS at DESY based on the infrastructure already existing. E.g. it is necessary for DESY to interact with RFID based self service terminals, barcode based library cards and external patrons who have not DESY account etc.

  13. Integrated Library System (ILS): An Option for Library and ...

    African Journals Online (AJOL)

    The purpose of this paper is to review the capability of integrated library system as an option for managing library information resources using Koha ILS used at Babcock University as a case study. The paper is a review of the functions and performance of Koha ILS. It also includes personal experiences of librarians and the ...

  14. Automation of library system: A study of John Harris library ...

    African Journals Online (AJOL)

    Information Technologist (The) ... and querying library records, maintains defaulting patrons overdue fines account while the OPAC empl oy ... The automated system generates reports necessary for an informed management decision making.

  15. An automated library financial management system

    Science.gov (United States)

    Dueker, S.; Gustafson, L.

    1977-01-01

    A computerized library acquisition system developed for control of informational materials acquired at NASA Ames Research Center is described. The system monitors the acquisition of both library and individual researchers' orders and supplies detailed financial, statistical, and bibliographical information. Applicability for other libraries and the future availability of the program is discussed.

  16. SECURING DIGITIZED LIBRARY CIRCULATORY SYSTEM | Olaniyi ...

    African Journals Online (AJOL)

    Journal Home > Vol 35, No 3 (2016) > ... Despite the potential benefits of Information and Communication Technology (ICT) in library automation, ... The widespread application of the developed system on smart library circulation unit would ...

  17. Afghanistan Digital Library Initiative: Revitalizing an Integrated Library System

    Directory of Open Access Journals (Sweden)

    Yan HAN

    2007-12-01

    Full Text Available This paper describes an Afghanistan digital library initiative of building an integrated library system (ILS for Afghanistan universities and colleges based on open-source software. As one of the goals of the Afghan eQuality Digital Libraries Alliance, the authors applied systems analysis approach, evaluated different open-source ILSs, and customized the selected software to accommodate users’ needs. Improvements include Arabic and Persian language support, user interface changes, call number label printing, and ISBN-13 support. To our knowledge, this ILS is the first at a large academic library running on open-source software.

  18. Evaluating Library Staff: A Performance Appraisal System.

    Science.gov (United States)

    Belcastro, Patricia

    This manual provides librarians and library managers with a performance appraisal system that measures staff fairly and objectively and links performance to the goals of the library. The following topics are addressed: (1) identifying expectations for quality service or standards of performance; (2) the importance of a library's code of service,…

  19. How to Evaluate Integrated Library Automation Systems.

    Science.gov (United States)

    Powell, James R.; Slach, June E.

    1985-01-01

    This paper describes methodology used in compiling a list of candidate integrated library automation systems at a corporate technical library. Priorities for automation, identification of candidate systems, the filtering process, information for suppliers, software and hardware considerations, on-site evaluations, and final system selection are…

  20. Case Studies in Library Computer Systems.

    Science.gov (United States)

    Palmer, Richard Phillips

    Twenty descriptive case studies of computer applications in a variety of libraries are presented in this book. Computerized circulation, serial and acquisition systems in public, high school, college, university and business libraries are included. Each of the studies discusses: 1) the environment in which the system operates, 2) the objectives of…

  1. An Integrated Library System: Preliminary Considerations.

    Science.gov (United States)

    Neroda, Edward

    Noting difficulties experienced by small to medium sized colleges in acquiring integrated library computer systems, this position paper outlines issues related to the subject with the intention of increasing familiarity and interest in integrated library systems. The report includes: a brief review of technological advances as they relate to…

  2. Synthetic promoter libraries- tuning of gene expression

    DEFF Research Database (Denmark)

    Hammer, Karin; Mijakovic, Ivan; Jensen, Peter Ruhdal

    2006-01-01

    knockout and strong overexpression. However, applications such as metabolic optimization and control analysis necessitate a continuous set of expression levels with only slight increments in strength to cover a specific window around the wildtype expression level of the studied gene; this requirement can......The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene...

  3. On the Recommender System for University Library

    Science.gov (United States)

    Fu, Shunkai; Zhang, Yao; Seinminn

    2013-01-01

    Libraries are important to universities, and they have two primary features: readers as well as collections are highly professional. In this study, based on the experimental study with five millions of users' borrowing records, our discussion covers: (1) the necessity of recommender system for university libraries; (2) collaborative filtering (CF)…

  4. Library Systems: FY 1992-2012 Public Libraries Survey Trends (Administrative Entity)

    Data.gov (United States)

    Institute of Museum and Library Services — Find key information on trends in library systems around the United States from FY 1992 to FY 2012.These data include imputed values for libraries that did not...

  5. Consumer Health Information Provision in Rural Public Libraries: A Comparison of Two Library Systems

    Science.gov (United States)

    Flaherty, Mary Grace

    2013-01-01

    To better understand health information provision in the public library setting, two cooperative library systems that serve primarily rural populations in upstate New York were studied. The central library in one of those systems established a consumer health information center (CHIC) in 1999. In the other system, the central library does not have…

  6. Experimental OAI-Based Digital Library Systems

    Science.gov (United States)

    Nelson, Michael L. (Editor); Maly, Kurt (Editor); Zubair, Mohammad (Editor); Rusch-Feja, Diann (Editor)

    2002-01-01

    The objective of Open Archives Initiative (OAI) is to develop a simple, lightweight framework to facilitate the discovery of content in distributed archives (http://www.openarchives.org). The focus of the workshop held at the 5th European Conference on Research and Advanced Technology for Digital Libraries (ECDL 2001) was to bring researchers in the area of digital libraries who are building OAI based systems so as to share their experiences, problems they are facing, and approaches they are taking to address them. The workshop consisted of invited talks from well-established researchers working in building OAI based digital library system along with short paper presentations.

  7. Metadata In, Library Out. A Simple, Robust Digital Library System

    Directory of Open Access Journals (Sweden)

    Tonio Loewald

    2010-06-01

    Full Text Available Tired of being held hostage to expensive systems that did not meet our needs, the University of Alabama Libraries developed an XML schema-agnostic, light-weight digital library delivery system based on the principles of "Keep It Simple, Stupid!" Metadata and derivatives reside in openly accessible web directories, which support the development of web agents and new usability software, as well as modification and complete retrieval at any time. The file name structure is echoed in the file system structure, enabling the delivery software to make inferences about relationships, sequencing, and complex object structure without having to encapsulate files in complex metadata schemas. The web delivery system, Acumen, is built of PHP, JSON, JavaScript and HTML5, using MySQL to support fielded searching. Recognizing that spreadsheets are more user-friendly than XML, an accompanying widget, Archivists Utility, transforms spreadsheets into MODS based on rules selected by the user. Acumen, Archivists Utility, and all supporting software scripts will be made available as open source.

  8. Awareness and usage of automated library systems by patrons in ...

    African Journals Online (AJOL)

    The emergence of automated information systems in libraries has changed the landscape of library environment. This paper assessed awareness and usage of automated information systems in two academic libraries in Nigeria; University of Lagos Main Library and Keneth Dike Library, University of Ibadan. Survey ...

  9. Internal Control Systems in the Library Environment.

    Science.gov (United States)

    Begg, Robert T.

    1985-01-01

    Examines system for safeguarding assets and guaranteeing reliability of library's financial records within context of management functions that comprise accounting controls: planning by budget or other standard of comparison; operation of effective accounting and record-keeping system; personnel management practices. Cash controls as example of…

  10. The JPL Library information retrieval system

    Science.gov (United States)

    Walsh, J.

    1975-01-01

    The development, capabilities, and products of the computer-based retrieval system of the Jet Propulsion Laboratory Library are described. The system handles books and documents, produces a book catalog, and provides a machine search capability. Programs and documentation are available to the public through NASA's computer software dissemination program.

  11. Selecting and Planning for an Automated Library System: Guidelines for Libraries.

    Science.gov (United States)

    Piccininni, James

    Guidelines are given for automating a library. Issues arising in automation are illustrated through the experience of the Doherty Library of the University of St. Thomas, Houston (Texas). The first step is to decide what type of system is right for the needs of the library and its patrons. In considering vendors of systems, it is important to…

  12. Design Principles for a Comprehensive Library System.

    Science.gov (United States)

    Uluakar, Tamer; And Others

    1981-01-01

    Describes an online design featuring circulation control, catalog access, and serial holdings that uses an incremental approach to system development. Utilizing a dedicated computer, this second of three releases pays particular attention to present and predicted computing capabilities as well as trends in library automation. (Author/RAA)

  13. Balancing gene expression without library construction via a reusable sRNA pool.

    Science.gov (United States)

    Ghodasara, Amar; Voigt, Christopher A

    2017-07-27

    Balancing protein expression is critical when optimizing genetic systems. Typically, this requires library construction to vary the genetic parts controlling each gene, which can be expensive and time-consuming. Here, we develop sRNAs corresponding to 15nt 'target' sequences that can be inserted upstream of a gene. The targeted gene can be repressed from 1.6- to 87-fold by controlling sRNA expression using promoters of different strength. A pool is built where six sRNAs are placed under the control of 16 promoters that span a ∼103-fold range of strengths, yielding ∼107 combinations. This pool can simultaneously optimize up to six genes in a system. This requires building only a single system-specific construct by placing a target sequence upstream of each gene and transforming it with the pre-built sRNA pool. The resulting library is screened and the top clone is sequenced to determine the promoter controlling each sRNA, from which the fold-repression of the genes can be inferred. The system is then rebuilt by rationally selecting parts that implement the optimal expression of each gene. We demonstrate the versatility of this approach by using the same pool to optimize a metabolic pathway (β-carotene) and genetic circuit (XNOR logic gate). © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  14. Arabic Natural Language Processing System Code Library

    Science.gov (United States)

    2014-06-01

    Adelphi, MD 20783-1197 This technical note provides a brief description of a Java library for Arabic natural language processing ( NLP ) containing code...for training and applying the Arabic NLP system described in the paper "A Cross-Task Flexible Transition Model for Arabic Tokenization, Affix...and also English) natural language processing ( NLP ), containing code for training and applying the Arabic NLP system described in Stephen Tratz’s

  15. Selecting a Relational Database Management System for Library Automation Systems.

    Science.gov (United States)

    Shekhel, Alex; O'Brien, Mike

    1989-01-01

    Describes the evaluation of four relational database management systems (RDBMSs) (Informix Turbo, Oracle 6.0 TPS, Unify 2000 and Relational Technology's Ingres 5.0) to determine which is best suited for library automation. The evaluation criteria used to develop a benchmark specifically designed to test RDBMSs for libraries are discussed. (CLB)

  16. Nuclear libraries for SCALE5.1 system

    International Nuclear Information System (INIS)

    Vertes, P.

    2009-01-01

    Codes for preparing master and working AMPX libraries and point-wise nuclear libraries for SCALE5.1 system have been created. Master and working libraries are constructed from multigroup library in matxs form which are produced by means of the NJOY code. The point-wise cross-section library is derived from pend files obtained also by NJOY. The AMPX libraries may contain neutron, gamma production and gamma transport data, as well. The produced master libraries can be used either with stand-alone functional modules or with control modules. An assistant package of programs also has been developed in order to facilitate the usage of NJOY. (Authors)

  17. Nuclear libraries for SCALE5.1 system

    International Nuclear Information System (INIS)

    Vertes, P.

    2009-01-01

    Codes for preparing master and working AMPX libraries and point-wise (PW) nuclear libraries for SCALE5.1 system have been created. Master and working libraries are constructed from multigroup library in matxs form which are produced by means of the NJOY code. The PW cross-section library is derived from pend files obtained also by NJOY. The AMPX libraries may contain neutron, gamma production and gamma transport data, as well. The produced master libraries can be used either with stand-alone functional modules or with control modules. An assistant package of programs also has been developed in order to facilitate the usage of NJOY. (author)

  18. Enhanced digital library system that supports sustainable knowledge

    African Journals Online (AJOL)

    Enhanced digital library system that supports sustainable knowledge: A focus ... This research work provides a Web-Based University library, ability to access the ... and generates pins to authorize bonafide students and staff of the University.

  19. Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

    Directory of Open Access Journals (Sweden)

    Eddy Edward M

    2007-07-01

    Full Text Available Abstract Background The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes. Results We analyzed the mouse spermatocyte UniGene library containing 2155 gene-oriented transcript clusters. We predict that 11% of these genes are testis-specific and systematically identified 24 authentic genes specifically and abundantly expressed in the testis via in silico and in vitro approaches. Northern blot analysis disclosed various transcript characteristics, such as expression level, size and the presence of isoform. Expression analysis revealed developmentally regulated and stage-specific expression patterns in all of the genes. We further analyzed the genes at the protein and cellular levels. Transfection assays performed using GC-2 cells provided information on the cellular characteristics of the gene products. In addition, antibodies were generated against proteins encoded by some of the genes to facilitate their identification and characterization in spermatogenic cells and sperm. Our data suggest that a number of the gene products are implicated in transcriptional regulation, nuclear integrity, sperm structure and motility, and fertilization. In particular, we found for the first time that Mm.333010, predicted to contain a trypsin-like serine protease domain, is a sperm acrosomal protein. Conclusion We identify 24 authentic genes with spermatogenic cell-specific expression, and provide comprehensive information about the genes. Our findings establish a new basis for future investigation into molecular mechanisms underlying male reproduction.

  20. Development of automatic editing system for MCNP library 'autonj'

    International Nuclear Information System (INIS)

    Maekawa, Fujio; Sakurai, Kiyoshi; Kume, Etsuo; Nomura, Yasushi; Kosako, Kazuaki; Kawasaki, Nobuo; Naito, Yoshitaka

    1999-12-01

    As an activity of the MCNP High-Temperature Library Production Working Group under the Nuclear Code Evaluation Special Committee of Nuclear Code Committee, the automatic editing system for MCNP library 'autonj' was developed. The autonj includes the NJOY-97 code as its main body, and is a system that enables us to easily produce cross section libraries for MCNP from evaluated nuclear data files such as JENDL-3.2. A temperature dependent library at six temperature points based on JENDL-3.2 was produced by using autonj. The autonj system and the temperature dependent library were installed on the JAERI AP3000 computer. (author)

  1. Library Systems Office Organization. SPEC Kit and SPEC Flyer 211.

    Science.gov (United States)

    Muir, Scott P., Comp.

    The roles and responsibilities of the library systems officer continues to change as libraries move beyond the automation of library functions to offering resources in electronic formats and electronic access to information about collections beyond the walls of the home institution. This survey was designed to collect data and document some of the…

  2. Managing library and information systems in the digital world ...

    African Journals Online (AJOL)

    This paper discusses the challenges and opportunities towards managing library and information system in relation to access the digital world's knowledge. Academic libraries are expected to provide access to information and digital material resources available within their reach. However, the Nigerian libraries especially ...

  3. Open Source Library Management Systems: A Multidimensional Evaluation

    Science.gov (United States)

    Balnaves, Edmund

    2008-01-01

    Open source library management systems have improved steadily in the last five years. They now present a credible option for small to medium libraries and library networks. An approach to their evaluation is proposed that takes account of three additional dimensions that only open source can offer: the developer and support community, the source…

  4. Research on Current Trends and Developments in Library Automation Systems.

    Science.gov (United States)

    Clyde, Laurel A.

    A research project was undertaken in 1992 in order to identify, describe, and analyze current trends and developments in library automation systems and library software. The starting point is work conducted in 1988/1989 that formed the foundation of the book "Computer Software for School Libraries: A Directory." A 1992 survey of software…

  5. Map it @ WSU: Development of a Library Mapping System for Large Academic Libraries

    Directory of Open Access Journals (Sweden)

    Paul Gallagher

    2010-06-01

    Full Text Available The Wayne State Library System launched its library mapping application in February 2010, designed to help locate materials in the five WSU libraries. The system works within the catalog to show the location of materials, as well as provides a web form for use at the reference desk. Developed using PHP and MySQL, it requires only minimal effort to update using a unique call number overlay mechanism. In addition to mapping shelved materials, the system provides information for any of the over three hundred collections held by the WSU Libraries. Patrons can do more than just locate a book on a shelf: they can learn where to locate reserve items, how to access closed collections, or get driving maps to extension center libraries. The article includes a discussion of the technology reviewed and chosen during development, an overview of the system architecture, and lessons learned during development.

  6. Library Automation Alternatives in 1996 and User Satisfaction Ratings of Library Users by Operating System.

    Science.gov (United States)

    Cibbarelli, Pamela

    1996-01-01

    Examines library automation product introductions and conversions to new operating systems. Compares user satisfaction ratings of the following library software packages: DOS/Windows, UNIX, Macintosh, and DEC VAX/VMS. Software is rated according to documentation, service/support, training, product reliability, product capabilities, ease of use,…

  7. Factors Affecting the Full Use of Library and Information Management Systems by Library Personnel

    Science.gov (United States)

    Skretas, Georgios

    2005-01-01

    Purpose: To provide a general list of factors that affects and determines the full use of library information management systems (LIMS) by library staff. Design/methodology/approach: The factors, which were identified mainly during participation in the implementation of automation projects in Greece, are listed and briefly analysed in categories…

  8. Introduction of library administration system using an office computer in the smallscale library

    International Nuclear Information System (INIS)

    Itabashi, Keizo; Ishikawa, Masashi

    1984-01-01

    Research Information Center was established in Fusion Research Center at Naka site as a new section of Department of Technical Information of Japan Atomic Energy Research Institute. A library materials management system utilizing an office computer was introduced to provide good services. The system is a total-system centered on services at counter except purchase business and the serviced materials are books, reports, journals and pamphlets. The system has produced good effects on many aspects, e.g. a significantly easy inventory of library materials, and complete removal of user's handwriting for borrowing materials, by using an optical chracter recognition handscanner. Those improvements have resulted in better image of the library. (author)

  9. XML: How It Will Be Applied to Digital Library Systems.

    Science.gov (United States)

    Kim, Hyun-Hee; Choi, Chang-Seok

    2000-01-01

    Shows how XML is applied to digital library systems. Compares major features of XML with those of HTML and describes an experimental XML-based metadata retrieval system, which is based on the Dublin Core and is designed as a subsystem of the Korean Virtual Library and Information System (VINIS). (Author/LRW)

  10. Advanced Transport Operating System (ATOPS) utility library software description

    Science.gov (United States)

    Clinedinst, Winston C.; Slominski, Christopher J.; Dickson, Richard W.; Wolverton, David A.

    1993-01-01

    The individual software processes used in the flight computers on-board the Advanced Transport Operating System (ATOPS) aircraft have many common functional elements. A library of commonly used software modules was created for general uses among the processes. The library includes modules for mathematical computations, data formatting, system database interfacing, and condition handling. The modules available in the library and their associated calling requirements are described.

  11. An Affordable Microcomputer Library Information System Developed by Georgetown University.

    Science.gov (United States)

    Broering, Naomi C.

    1984-01-01

    Discusses development of a library information system by Georgetown University Medical Center's Dahlgren Memorial Library; describes its components, including online catalog and OCLC interface, circulation, serials control, acquisitions, networking/interlibrary loans, word processing and mini-Medline system; and discusses user reaction, staff…

  12. Booth Library On-Line Circulation System (BLOC

    Directory of Open Access Journals (Sweden)

    Paladugu V. Rao

    1971-06-01

    Full Text Available An on-line circulation system developed at a relatively small university library demonstrates that academic libraries with limited funds can develop automated systems utilizing parent institution's computer facilities in a time-sharing mode. In operation since September 1968, using an IBM 360/50 computer and associated peripheral equipment, it provides control over all stack books.

  13. Providing Access to Library Automation Systems for Students with Disabilities.

    Science.gov (United States)

    California Community Colleges, Sacramento. High-Tech Center for the Disabled.

    This document provides information on the integration of assistive computer technologies and library automation systems at California Community Colleges in order to ensure access for students with disabilities. Topics covered include planning, upgrading, purchasing, implementing and using these technologies with library systems. As information…

  14. Expert Systems for Libraries at SCIL [Small Computers in Libraries]'88.

    Science.gov (United States)

    Kochtanek, Thomas R.; And Others

    1988-01-01

    Six brief papers on expert systems for libraries cover (1) a knowledge-based approach to database design; (2) getting started in expert systems; (3) using public domain software to develop a business reference system; (4) a music cataloging inquiry system; (5) linguistic analysis of reference transactions; and (6) a model of a reference librarian.…

  15. The Vendors' Corner: Biblio-Techniques' Library and Information System (BLIS).

    Science.gov (United States)

    Library Software Review, 1984

    1984-01-01

    Describes online catalog and integrated library computer system designed to enhance Washington Library Network's software. Highlights include system components; implementation options; system features (integrated library functions, database design, system management facilities); support services (installation and training, software maintenance and…

  16. Construction of human antibody gene libraries and selection of antibodies by phage display.

    Science.gov (United States)

    Frenzel, André; Kügler, Jonas; Wilke, Sonja; Schirrmann, Thomas; Hust, Michael

    2014-01-01

    Antibody phage display is the most commonly used in vitro selection technology and has yielded thousands of useful antibodies for research, diagnostics, and therapy.The prerequisite for successful generation and development of human recombinant antibodies using phage display is the construction of a high-quality antibody gene library. Here, we describe the methods for the construction of human immune and naive scFv gene libraries.The success also depends on the panning strategy for the selection of binders from these libraries. In this article, we describe a panning strategy that is high-throughput compatible and allows parallel selection in microtiter plates.

  17. CSIR's new integrated electronic library information-system

    CSIR Research Space (South Africa)

    Michie, A

    1995-08-01

    Full Text Available The CSIR has developed a CDROM-based electronic library information system which provides the ability to reproduce and search for published information and colour brochures on the computer screen. The system integrates this information with online...

  18. Integrated library system in the library of Japan Atomic Energy Research Institute

    International Nuclear Information System (INIS)

    Yonezawa, Minoru; Mineo, Yukinobu; Itabashi, Keizo

    1987-01-01

    Integrated library system has been developed using a stand-alone mini-computer in the Japan Atomic Energy Research Institute library. This system consists of three subsystems for serials control, books acquisition and circulation control. Serials control subsystem deals with subscription, acquisition, claiming and inquiry of journals. This has been operating since the beginning of 1985. Book acquisition sub-system, which has been started since April 1986, deals with accounting and cataloguing of books. Circulation control sub-system deals with circulation, statistics compilation, book inventory and retrieval, which has been operating since April 1987. This system contributes greatly not only to the reduction of the circulation work load but also to the promotion of the library services. However, the convenience in circulation processing should be improved for materials without catalogue information stored in the computer. The pertinence for maximum number of books retrieved has to be also reconsidered. (author)

  19. Knowledge Organisation Systems in North American Digital Library Collections

    Science.gov (United States)

    Shiri, Ali; Chase-Kruszewski, Sarah

    2009-01-01

    Purpose: The purpose of this paper is to report an investigation into the types of knowledge organisation systems (KOSs) utilised in North American digital library collections. Design/methodology/approach: The paper identifies, analyses and deep scans online North American hosted digital libraries. It reviews the literature related to the…

  20. The construction of knowledge service system in professional libraries

    International Nuclear Information System (INIS)

    Zhang Xue

    2014-01-01

    In this paper, the challenges for the professional libraries under the new situation are pointed out. Combined with characteristics of knowledge service, its hierarchical structure is proposed and elaborated. It also describes the practices and outcomes obtained from the construction of knowledge service system of nuclear science and technology library. Recommendations for its future work are also presented. (author)

  1. Library Information System Time-Sharing (LISTS) Project. Final Report.

    Science.gov (United States)

    Black, Donald V.

    The Library Information System Time-Sharing (LISTS) experiment was based on three innovations in data processing technology: (1) the advent of computer time-sharing on third-generation machines, (2) the development of general-purpose file-management software and (3) the introduction of large, library-oriented data bases. The main body of the…

  2. Looking Toward the Future of Library Technology. The Systems Librarian

    Science.gov (United States)

    Breeding, Marshall

    2005-01-01

    This article discusses trends in five areas relating to software developed for libraries, and based on these trends, the author's predictions for developments that might play out in the next few years. The author's predictions, based on his own empirical observations, include: (1) the integrated library system (ILS) will be reintegrated; (2) the…

  3. Redistributing Stock in Library Systems with a Depot

    NARCIS (Netherlands)

    van der Heide, Gerlach; Roodbergen, Kees Jan; van Foreest, Nicky

    Public library organizations often utilize depots for carrying out shipments to libraries in case of stock-outs and for storing low demand rental items at low cost. Similar systems may be employed by rental companies for other rental products such as tools, DVDs, and jewelry. Since shipments deplete

  4. Consumer health information partnerships: the health science library and multitype library system.

    Science.gov (United States)

    Hollander, S

    1996-04-01

    The University of Illinois Library of the Health Sciences at Rockford (LHS-Rockford) long has honored a commitment to serving the health information needs of the greater Rockford community. Utilization data collected over the past five years indicate that approximately 50% of reference transactions involve persons not affiliated with the university. In early 1994, LHS-Rockford submitted a proposal to the Northern Illinois Library System (NILS), a multitype system spanning twelve counties in northwestern Illinois, asking to serve as a resource library for improving medical and health information services provided by the 138 NILS member libraries. The NILS funded this pilot project as part of an effort to implement a new strategic plan, which encouraged member libraries to form networks to provide reference back-up service. LHS-Rockford acquired InfoTrac's Health Reference Center, a consumer health information database, and set up a dedicated workstation near the information and circulation desk. Referral guidelines were established and the project was promoted among NILS member libraries. Activities were documented in order to track project success in terms of referrals and outcomes. The demonstration project was very successful, and it proves public consumers seeking health information can benefit greatly from this type of cooperative arrangement.

  5. Introduction of library administration system using an office computer in the smallscale library

    Energy Technology Data Exchange (ETDEWEB)

    Itabashi, Keizo; Ishikawa, Masashi

    1984-01-01

    Research Information Center was established in Fusion Research Center at Naka site as a new section of Department of Technical Information of Japan Atomic Energy Research Institute. A library materials management system utilizing an office computer was introduced to provide good services. The system is a total-system centered on services at counter except purchase business and the serviced materials are books, reports, journals and pamphlets. The system has produced good effects on many aspects, e.g. a significantly easy inventory of library materials, and complete removal of user's handwriting for borrowing materials, by using an optical chracter recognition handscanner. Those improvements have resulted in better image of the library.

  6. Planning an Integrated On-Line Library system (IOLS)

    Science.gov (United States)

    1989-03-01

    Logical Workflow for Circulation of Library Materials ............. 14 Figure 9. Detail of Circulation of Libary Materials ...................... 15...Operating Honolulu, HI 96826 System (808) 947-4441 DATA RESEARCH ASSOCIATES, Inc. (ATLAS) 9270 Olive Blvd. St. Louis, MO 01775 DIGITAL EQUIPMENT CORP... DIGITAL EQUIPMENT CORP. Stow, MA 01775 (617) 897-7163 EYRING LIBRARY SYSTEMS (CARL) 5280 S. West, Suite E260 Salt Lake City, UT 84107 TANDEM SYSTEMS

  7. K-shuff: A Novel Algorithm for Characterizing Structural and Compositional Diversity in Gene Libraries.

    Science.gov (United States)

    Jangid, Kamlesh; Kao, Ming-Hung; Lahamge, Aishwarya; Williams, Mark A; Rathbun, Stephen L; Whitman, William B

    2016-01-01

    K-shuff is a new algorithm for comparing the similarity of gene sequence libraries, providing measures of the structural and compositional diversity as well as the significance of the differences between these measures. Inspired by Ripley's K-function for spatial point pattern analysis, the Intra K-function or IKF measures the structural diversity, including both the richness and overall similarity of the sequences, within a library. The Cross K-function or CKF measures the compositional diversity between gene libraries, reflecting both the number of OTUs shared as well as the overall similarity in OTUs. A Monte Carlo testing procedure then enables statistical evaluation of both the structural and compositional diversity between gene libraries. For 16S rRNA gene libraries from complex bacterial communities such as those found in seawater, salt marsh sediments, and soils, K-shuff yields reproducible estimates of structural and compositional diversity with libraries greater than 50 sequences. Similarly, for pyrosequencing libraries generated from a glacial retreat chronosequence and Illumina® libraries generated from US homes, K-shuff required >300 and 100 sequences per sample, respectively. Power analyses demonstrated that K-shuff is sensitive to small differences in Sanger or Illumina® libraries. This extra sensitivity of K-shuff enabled examination of compositional differences at much deeper taxonomic levels, such as within abundant OTUs. This is especially useful when comparing communities that are compositionally very similar but functionally different. K-shuff will therefore prove beneficial for conventional microbiome analysis as well as specific hypothesis testing.

  8. Cloud Computing Platform for an Online Model Library System

    Directory of Open Access Journals (Sweden)

    Mingang Chen

    2013-01-01

    Full Text Available The rapid developing of digital content industry calls for online model libraries. For the efficiency, user experience, and reliability merits of the model library, this paper designs a Web 3D model library system based on a cloud computing platform. Taking into account complex models, which cause difficulties in real-time 3D interaction, we adopt the model simplification and size adaptive adjustment methods to make the system with more efficient interaction. Meanwhile, a cloud-based architecture is developed to ensure the reliability and scalability of the system. The 3D model library system is intended to be accessible by online users with good interactive experiences. The feasibility of the solution has been tested by experiments.

  9. A Challenge to Change: Necessary Changes in the Library Classification System for the Chicago Public Schools.

    Science.gov (United States)

    Williams, Florence M.

    This report addresses the feasibility of changing the classification of library materials in the Chicago Public School libraries from the Dewey Decimal classification system (DDC) to the Library of Congress system (LC), thus patterning the city school libraries after the Chicago Public Library and strengthening the existing close relationship…

  10. Identification of genes and pathways related to phenol degradation in metagenomic libraries from petroleum refinery wastewater.

    Directory of Open Access Journals (Sweden)

    Cynthia C Silva

    Full Text Available Two fosmid libraries, totaling 13,200 clones, were obtained from bioreactor sludge of petroleum refinery wastewater treatment system. The library screening based on PCR and biological activity assays revealed more than 400 positive clones for phenol degradation. From these, 100 clones were randomly selected for pyrosequencing in order to evaluate the genetic potential of the microorganisms present in wastewater treatment plant for biodegradation, focusing mainly on novel genes and pathways of phenol and aromatic compound degradation. The sequence analysis of selected clones yielded 129,635 reads at an estimated 17-fold coverage. The phylogenetic analysis showed Burkholderiales and Rhodocyclales as the most abundant orders among the selected fosmid clones. The MG-RAST analysis revealed a broad metabolic profile with important functions for wastewater treatment, including metabolism of aromatic compounds, nitrogen, sulphur and phosphorus. The predicted 2,276 proteins included phenol hydroxylases and cathecol 2,3- dioxygenases, involved in the catabolism of aromatic compounds, such as phenol, byphenol, benzoate and phenylpropanoid. The sequencing of one fosmid insert of 33 kb unraveled the gene that permitted the host, Escherichia coli EPI300, to grow in the presence of aromatic compounds. Additionally, the comparison of the whole fosmid sequence against bacterial genomes deposited in GenBank showed that about 90% of sequence showed no identity to known sequences of Proteobacteria deposited in the NCBI database. This study surveyed the functional potential of fosmid clones for aromatic compound degradation and contributed to our knowledge of the biodegradative capacity and pathways of microbial assemblages present in refinery wastewater treatment system.

  11. Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

    DEFF Research Database (Denmark)

    Nielsen, Jeppe Lund; Schramm, Andreas; Bernhard, Anne E.

    2004-01-01

    for Systems Biology,3 Seattle, Washington, and Department of Ecological Microbiology, University of Bayreuth, Bayreuth, Germany2 A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting......  FLOW CYTOMETRY-ASSISTED CLONING OF SPECIFIC SEQUENCE MOTIFS FROM COMPLEX 16S RRNA GENE LIBRARIES Jeppe L. Nielsen,1 Andreas Schramm,1,2 Anne E. Bernhard,1 Gerrit J. van den Engh,3 and David A. Stahl1* Department of Civil and Environmental Engineering, University of Washington,1 and Institute......-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.  ...

  12. Library

    OpenAIRE

    Dulaney, Ronald E. Jr.

    1997-01-01

    This study began with the desire to design a public town library of the future and became a search for an inkling of what is essential to Architecture. It is murky and full of contradictions. It asks more than it proposes, and the traces of its windings are better ordered through collage than logical synthesis. This study is neither a thesis nor a synthesis. When drawing out the measure of this study it may be beneficial to state what it attempts to place at the ...

  13. DMG-α--a computational geometry library for multimolecular systems.

    Science.gov (United States)

    Szczelina, Robert; Murzyn, Krzysztof

    2014-11-24

    The DMG-α library grants researchers in the field of computational biology, chemistry, and biophysics access to an open-sourced, easy to use, and intuitive software for performing fine-grained geometric analysis of molecular systems. The library is capable of computing power diagrams (weighted Voronoi diagrams) in three dimensions with 3D periodic boundary conditions, computing approximate projective 2D Voronoi diagrams on arbitrarily defined surfaces, performing shape properties recognition using α-shape theory and can do exact Solvent Accessible Surface Area (SASA) computation. The software is written mainly as a template-based C++ library for greater performance, but a rich Python interface (pydmga) is provided as a convenient way to manipulate the DMG-α routines. To illustrate possible applications of the DMG-α library, we present results of sample analyses which allowed to determine nontrivial geometric properties of two Escherichia coli-specific lipids as emerging from molecular dynamics simulations of relevant model bilayers.

  14. A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules

    Science.gov (United States)

    Popova, Blagovesta; Schubert, Steffen; Bulla, Ingo; Buchwald, Daniela; Kramer, Wilfried

    2015-01-01

    A major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants. The use of trinucleotides mixtures for controlled randomization results in superior library diversity and offers the ability to specify the type and distribution of the amino acids at each position. Here we describe the generation of a high diversity gene library using tHisF of the hyperthermophile Thermotoga maritima as a scaffold. Combining various rational criteria with contingency, we targeted 26 selected codons of the thisF gene sequence for randomization at a controlled level. We have developed a novel method of creating full-length gene libraries by combinatorial assembly of smaller sub-libraries. Full-length libraries of high diversity can easily be assembled on demand from smaller and much less diverse sub-libraries, which circumvent the notoriously troublesome long-term archivation and repeated proliferation of high diversity ensembles of phages or plasmids. We developed a generally applicable software tool for sequence analysis of mutated gene sequences that provides efficient assistance for analysis of library diversity. Finally, practical utility of the library was demonstrated in principle by assessment of the conformational stability of library members and isolating protein variants with HisF activity from it. Our approach integrates a number of features of nucleic acids synthetic chemistry, biochemistry and molecular genetics to a coherent, flexible and robust method of combinatorial gene synthesis. PMID:26355961

  15. Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization.

    Science.gov (United States)

    Jiang, Likun; You, Weiwei; Zhang, Xiaojun; Xu, Jian; Jiang, Yanliang; Wang, Kai; Zhao, Zixia; Chen, Baohua; Zhao, Yunfeng; Mahboob, Shahid; Al-Ghanim, Khalid A; Ke, Caihuan; Xu, Peng

    2016-02-01

    The small abalone (Haliotis diversicolor) is one of the most important aquaculture species in East Asia. To facilitate gene cloning and characterization, genome analysis, and genetic breeding of it, we constructed a large-insert bacterial artificial chromosome (BAC) library, which is an important genetic tool for advanced genetics and genomics research. The small abalone BAC library includes 92,610 clones with an average insert size of 120 Kb, equivalent to approximately 7.6× of the small abalone genome. We set up three-dimensional pools and super pools of 18,432 BAC clones for target gene screening using PCR method. To assess the approach, we screened 12 target genes in these 18,432 BAC clones and identified 16 positive BAC clones. Eight positive BAC clones were then sequenced and assembled with the next generation sequencing platform. The assembled contigs representing these 8 BAC clones spanned 928 Kb of the small abalone genome, providing the first batch of genome sequences for genome evaluation and characterization. The average GC content of small abalone genome was estimated as 40.33%. A total of 21 protein-coding genes, including 7 target genes, were annotated into the 8 BACs, which proved the feasibility of PCR screening approach with three-dimensional pools in small abalone BAC library. One hundred fifty microsatellite loci were also identified from the sequences for marker development in the future. The BAC library and clone pools provided valuable resources and tools for genetic breeding and conservation of H. diversicolor.

  16. Multiple Case Studies of Public Library Systems in New York State: Service Decision-Making Processes

    Science.gov (United States)

    Ren, Xiaoai

    2012-01-01

    This research examined the functions and roles of public library systems in New York State and the services they provide for individual libraries and the public. The dissertation further studied the service decision-making processes at three selected New York State cooperative public library systems. Public library systems have played an important…

  17. End-user satisfaction analysis on library management system unnes using technology acceptance model towards national standard of integrated library

    Science.gov (United States)

    Hardyanto, W.; Purwinarko, A.; Adhi, M. A.

    2018-03-01

    The library which is the gate of the University should be supported by the existence of an adequate information system, to provide excellent service and optimal to every user. Library management system that has been in existence since 2009 needs to be re-evaluated so that the system can meet the needs of both operator and Unnes user in particular, and users from outside Unnes in general. This study aims to evaluate and improve the existing library management system to produce a system that is accountable and able to meet the needs of end users, as well as produce a library management system that is integrated Unnes. Research is directed to produce evaluation report with Technology Acceptance Model (TAM) approach and library management system integrated with the national standard.

  18. Student project of optical system analysis API-library development

    Science.gov (United States)

    Ivanova, Tatiana; Zhukova, Tatiana; Dantcaranov, Ruslan; Romanova, Maria; Zhadin, Alexander; Ivanov, Vyacheslav; Kalinkina, Olga

    2017-08-01

    In the paper API-library software developed by students of Applied and Computer Optics Department (ITMO University) for optical system design is presented. The library performs paraxial and real ray tracing, calculates 3d order (Seidel) aberration and real ray aberration of axis and non-axis beams (wave, lateral, longitudinal, coma, distortion etc.) and finally, approximate wave aberration by Zernike polynomials. Real aperture can be calculated by considering of real rays tracing failure on each surface. So far we assume optical system is centered, with spherical or 2d order aspherical surfaces. Optical glasses can be set directly by refraction index or by dispersion coefficients. The library can be used for education or research purposes in optical system design area. It provides ready to use software functions for optical system simulation and analysis that developer can simply plug into their software development for different purposes, for example for some specific synthesis tasks or investigation of new optimization modes. In the paper we present an example of using the library for development of cemented doublet synthesis software based on Slusarev's methodology. The library is used in optical system optimization recipes course for deep studying of optimization model and its application for optical system design. Development of such software is an excellent experience for students and help to understanding optical image modeling and quality analysis. This development is organized as student group joint project. We try to organize it as a group in real research and development project, so each student has his own role in the project and then use whole library functionality in his own master or bachelor thesis. Working in such group gives students useful experience and opportunity to work as research and development engineer of scientific software in the future.

  19. Identification of immune protective genes of Eimeria maxima through cDNA expression library screening.

    Science.gov (United States)

    Yang, XinChao; Li, MengHui; Liu, JianHua; Ji, YiHong; Li, XiangRui; Xu, LiXin; Yan, RuoFeng; Song, XiaoKai

    2017-02-16

    Eimeria maxima is one of the most prevalent Eimeria species causing avian coccidiosis, and results in huge economic loss to the global poultry industry. Current control strategies, such as anti-coccidial medication and live vaccines have been limited because of their drawbacks. The third generation anticoccidial vaccines including the recombinant vaccines as well as DNA vaccines have been suggested as a promising alternative strategy. To date, only a few protective antigens of E. maxima have been reported. Hence, there is an urgent need to identify novel protective antigens of E. maxima for the development of neotype anticoccidial vaccines. With the aim of identifying novel protective genes of E. maxima, a cDNA expression library of E. maxima sporozoites was constructed using Gateway technology. Subsequently, the cDNA expression library was divided into 15 sub-libraries for cDNA expression library immunization (cDELI) using parasite challenged model in chickens. Protective sub-libraries were selected for the next round of screening until individual protective clones were obtained, which were further sequenced and analyzed. Adopting the Gateway technology, a high-quality entry library was constructed, containing 9.2 × 10 6 clones with an average inserted fragments length of 1.63 kb. The expression library capacity was 2.32 × 10 7 colony-forming units (cfu) with an average inserted fragments length of 1.64 Kb. The expression library was screened using parasite challenged model in chickens. The screening yielded 6 immune protective genes including four novel protective genes of EmJS-1, EmRP, EmHP-1 and EmHP-2, and two known protective genes of EmSAG and EmCKRS. EmJS-1 is the selR domain-containing protein of E. maxima whose function is unknown. EmHP-1 and EmHP-2 are the hypothetical proteins of E. maxima. EmRP and EmSAG are rhomboid-like protein and surface antigen glycoproteins of E. maxima respectively, and involved in invasion of the parasite. Our

  20. DICOM implementation on online tape library storage system

    Science.gov (United States)

    Komo, Darmadi; Dai, Hailei L.; Elghammer, David; Levine, Betty A.; Mun, Seong K.

    1998-07-01

    The main purpose of this project is to implement a Digital Image and Communications (DICOM) compliant online tape library system over the Internet. Once finished, the system will be used to store medical exams generated from U.S. ARMY Mobile ARMY Surgical Hospital (MASH) in Tuzla, Bosnia. A modified UC Davis implementation of DICOM storage class is used for this project. DICOM storage class user and provider are implemented as the system's interface to the Internet. The DICOM software provides flexible configuration options such as types of modalities and trusted remote DICOM hosts. Metadata is extracted from each exam and indexed in a relational database for query and retrieve purposes. The medical images are stored inside the Wolfcreek-9360 tape library system from StorageTek Corporation. The tape library system has nearline access to more than 1000 tapes. Each tape has a capacity of 800 megabytes making the total nearline tape access of around 1 terabyte. The tape library uses the Application Storage Manager (ASM) which provides cost-effective file management, storage, archival, and retrieval services. ASM automatically and transparently copies files from expensive magnetic disk to less expensive nearline tape library, and restores the files back when they are needed. The ASM also provides a crash recovery tool, which enable an entire file system restore in a short time. A graphical user interface (GUI) function is used to view the contents of the storage systems. This GUI also allows user to retrieve the stored exams and send the exams to anywhere on the Internet using DICOM protocols. With the integration of different components of the system, we have implemented a high capacity online tape library storage system that is flexible and easy to use. Using tape as an alternative storage media as opposed to the magnetic disk has the great potential of cost savings in terms of dollars per megabyte of storage. As this system matures, the Hospital Information Systems

  1. Analysis of an ordered, comprehensive STM mutant library in infectious Borrelia burgdorferi: insights into the genes required for mouse infectivity.

    Directory of Open Access Journals (Sweden)

    Tao Lin

    Full Text Available The identification of genes important in the pathogenesis of Lyme disease Borrelia has been hampered by exceedingly low transformation rates in low-passage, infectious organisms. Using the infectious, moderately transformable B. burgdorferi derivative 5A18NP1 and signature-tagged versions of the Himar1 transposon vector pGKT, we have constructed a defined transposon library for the efficient genome-wide investigation of genes required for wild-type pathogenesis, in vitro growth, physiology, morphology, and plasmid replication. To facilitate analysis, the insertion sites of 4,479 transposon mutants were determined by sequencing. The transposon insertions were widely distributed across the entire B. burgdorferi genome, with an average of 2.68 unique insertion sites per kb DNA. The 10 linear plasmids and 9 circular plasmids had insertions in 33 to 100 percent of their predicted genes. In contrast, only 35% of genes in the 910 kb linear chromosome had incapacitating insertions; therefore, the remaining 601 chromosomal genes may represent essential gene candidates. In initial signature-tagged mutagenesis (STM analyses, 434 mutants were examined at multiple tissue sites for infectivity in mice using a semi-quantitative, Luminex-based DNA detection method. Examples of genes found to be important in mouse infectivity included those involved in motility, chemotaxis, the phosphoenolpyruvate phosphotransferase system, and other transporters, as well as putative plasmid maintenance genes. Availability of this ordered STM library and a high-throughput screening method is expected to lead to efficient assessment of the roles of B. burgdorferi genes in the infectious cycle and pathogenesis of Lyme disease.

  2. Personalized Recommender System for Digital Libraries

    Science.gov (United States)

    Omisore, M. O.; Samuel, O. W.

    2014-01-01

    The huge amount of information available online has given rise to personalization and filtering systems. Recommender systems (RS) constitute a specific type of information filtering technique that present items according to user's interests. In this research, a web-based personalized recommender system capable of providing learners with books that…

  3. Management-retrieval code system of fission barrier parameter sub-library

    International Nuclear Information System (INIS)

    Zhang Limin; Su Zongdi; Ge Zhigang

    1995-01-01

    The fission barrier parameter (FBP) library, which is a sub-library of Chinese Evaluated Nuclear Parameter library (CENPL), stores various popular used fission barrier parameters from different historical period, and could retrieve the required fission barrier parameters by using the management retrieval code system of the FBP sub-library. The function, feature and operation instruction of the code system are described briefly

  4. Three-Dimensional Extension of a Digital Library Service System

    Science.gov (United States)

    Xiao, Long

    2010-01-01

    Purpose: The paper aims to provide an overall methodology and case study for the innovation and extension of a digital library, especially the service system. Design/methodology/approach: Based on the three-dimensional structure theory of the information service industry, this paper combines a comprehensive analysis with the practical experiences…

  5. Digital Libraries: The Next Generation in File System Technology.

    Science.gov (United States)

    Bowman, Mic; Camargo, Bill

    1998-01-01

    Examines file sharing within corporations that use wide-area, distributed file systems. Applications and user interactions strongly suggest that the addition of services typically associated with digital libraries (content-based file location, strongly typed objects, representation of complex relationships between documents, and extrinsic…

  6. Directory of Library Automation Software, Systems, and Services. 1998 Edition.

    Science.gov (United States)

    Cibbarelli, Pamela R., Ed.; Cibbarelli, Shawn E., Ed.

    This book includes basic information to locate and compare available options for library automation based on various criteria such as hardware requirements, operating systems, components and applications, and price, and provides the necessary contact information to allow further investigation. The major part of the directory lists 211 software…

  7. The Evolution of Discovery Systems in Academic Libraries: A Case Study at the University of Houston Libraries

    Science.gov (United States)

    Guajardo, Richard; Brett, Kelsey; Young, Frederick

    2017-01-01

    For the past several years academic libraries have been adopting discovery systems to provide a search experience that reflects user expectations and improves access to electronic resources. University of Houston Libraries has kept pace with this evolving trend by pursuing various discovery options; these include an open-source tool, a federated…

  8. De novo assembly and next-generation sequencing to analyse full-length gene variants from codon-barcoded libraries.

    Science.gov (United States)

    Cho, Namjin; Hwang, Byungjin; Yoon, Jung-ki; Park, Sangun; Lee, Joongoo; Seo, Han Na; Lee, Jeewon; Huh, Sunghoon; Chung, Jinsoo; Bang, Duhee

    2015-09-21

    Interpreting epistatic interactions is crucial for understanding evolutionary dynamics of complex genetic systems and unveiling structure and function of genetic pathways. Although high resolution mapping of en masse variant libraries renders molecular biologists to address genotype-phenotype relationships, long-read sequencing technology remains indispensable to assess functional relationship between mutations that lie far apart. Here, we introduce JigsawSeq for multiplexed sequence identification of pooled gene variant libraries by combining a codon-based molecular barcoding strategy and de novo assembly of short-read data. We first validate JigsawSeq on small sub-pools and observed high precision and recall at various experimental settings. With extensive simulations, we then apply JigsawSeq to large-scale gene variant libraries to show that our method can be reliably scaled using next-generation sequencing. JigsawSeq may serve as a rapid screening tool for functional genomics and offer the opportunity to explore evolutionary trajectories of protein variants.

  9. A Virtual Library for Instructional Systems Designers.

    Science.gov (United States)

    Kirk, James J.

    During the past three years, the role of the instructional systems designer has taken on greater significance for instructors. This is because many faculty members have been asked to put their entire courses online over the World Wide Web. Instructors are now creating a substantial percentage of the online courses with little or no background in…

  10. Dynamic combinatorial libraries: from exploring molecular recognition to systems chemistry.

    Science.gov (United States)

    Li, Jianwei; Nowak, Piotr; Otto, Sijbren

    2013-06-26

    Dynamic combinatorial chemistry (DCC) is a subset of combinatorial chemistry where the library members interconvert continuously by exchanging building blocks with each other. Dynamic combinatorial libraries (DCLs) are powerful tools for discovering the unexpected and have given rise to many fascinating molecules, ranging from interlocked structures to self-replicators. Furthermore, dynamic combinatorial molecular networks can produce emergent properties at systems level, which provide exciting new opportunities in systems chemistry. In this perspective we will highlight some new methodologies in this field and analyze selected examples of DCLs that are under thermodynamic control, leading to synthetic receptors, catalytic systems, and complex self-assembled supramolecular architectures. Also reviewed are extensions of the principles of DCC to systems that are not at equilibrium and may therefore harbor richer functional behavior. Examples include self-replication and molecular machines.

  11. The simulation library of the Belle II software system

    Science.gov (United States)

    Kim, D. Y.; Ritter, M.; Bilka, T.; Bobrov, A.; Casarosa, G.; Chilikin, K.; Ferber, T.; Godang, R.; Jaegle, I.; Kandra, J.; Kodys, P.; Kuhr, T.; Kvasnicka, P.; Nakayama, H.; Piilonen, L.; Pulvermacher, C.; Santelj, L.; Schwenker, B.; Sibidanov, A.; Soloviev, Y.; Starič, M.; Uglov, T.

    2017-10-01

    SuperKEKB, the next generation B factory, has been constructed in Japan as an upgrade of KEKB. This brand new e+ e- collider is expected to deliver a very large data set for the Belle II experiment, which will be 50 times larger than the previous Belle sample. Both the triggered physics event rate and the background event rate will be increased by at least 10 times than the previous ones, and will create a challenging data taking environment for the Belle II detector. The software system of the Belle II experiment is designed to execute this ambitious plan. A full detector simulation library, which is a part of the Belle II software system, is created based on Geant4 and has been tested thoroughly. Recently the library has been upgraded with Geant4 version 10.1. The library is behaving as expected and it is utilized actively in producing Monte Carlo data sets for various studies. In this paper, we will explain the structure of the simulation library and the various interfaces to other packages including geometry and beam background simulation.

  12. SLIB77, Source Library Data Compression and File Maintenance System

    International Nuclear Information System (INIS)

    Lunsford, A.

    1989-01-01

    Description of program or function: SLIB77 is a source librarian program designed to maintain FORTRAN source code in a compressed form on magnetic disk. The program was prepared to meet program maintenance requirements for ongoing program development and continual improvement of very large programs involving many programmers from a number of different organizations. SLIB77 automatically maintains in one file the source of the current program as well as all previous modifications. Although written originally for FORTRAN programs, SLIB77 is suitable for use with data files, text files, operating systems, and other programming languages, such as Ada, C and COBOL. It can handle libraries with records of up to 160-characters. Records are grouped into DECKS and assigned deck names by the user. SLIB77 assigns a number to each record in each DECK. Records can be deleted or restored singly or as a group within each deck. Modification records are grouped and assigned modification identification names by the user. The program assigns numbers to each new record within the deck. The program has two modes of execution, BATCH and EDIT. The BATCH mode is controlled by an input file and is used to make changes permanent and create new library files. The EDIT mode is controlled by interactive terminal input and a built-in line editor is used for modification of single decks. Transferring of a library from one computer system to another is accomplished using a Portable Library File created by SLIB77 in a BATCH run

  13. Advancing Eucalyptus genomics: identification and sequencing of lignin biosynthesis genes from deep-coverage BAC libraries

    Directory of Open Access Journals (Sweden)

    Kudrna David

    2011-03-01

    Full Text Available Abstract Background Eucalyptus species are among the most planted hardwoods in the world because of their rapid growth, adaptability and valuable wood properties. The development and integration of genomic resources into breeding practice will be increasingly important in the decades to come. Bacterial artificial chromosome (BAC libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing. Results We describe the construction and characterization of two deep-coverage BAC libraries EG_Ba and EG_Bb obtained from nuclear DNA fragments of E. grandis (clone BRASUZ1 digested with HindIII and BstYI, respectively. Genome coverages of 17 and 15 haploid genome equivalents were estimated for EG_Ba and EG_Bb, respectively. Both libraries contained large inserts, with average sizes ranging from 135 Kb (Eg_Bb to 157 Kb (Eg_Ba, very low extra-nuclear genome contamination providing a probability of finding a single copy gene ≥ 99.99%. Libraries were screened for the presence of several genes of interest via hybridizations to high-density BAC filters followed by PCR validation. Five selected BAC clones were sequenced and assembled using the Roche GS FLX technology providing the whole sequence of the E. grandis chloroplast genome, and complete genomic sequences of important lignin biosynthesis genes. Conclusions The two E. grandis BAC libraries described in this study represent an important milestone for the advancement of Eucalyptus genomics and forest tree research. These BAC resources have a highly redundant genome coverage (> 15×, contain large average inserts and have a very low percentage of clones with organellar DNA or empty vectors. These publicly available BAC libraries are thus suitable for a broad range of applications in genetic and genomic research in Eucalyptus and possibly in related species of Myrtaceae

  14. Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

    Science.gov (United States)

    Byk, G; Soto, J; Mattler, C; Frederic, M; Scherman, D

    1998-01-01

    The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthesized a secondary library of mono-functionalized poly-(guanidinium)amines generated from a library of mono-functionalized polyamines applying the concept of "libraries from libraries." The method allows a quick and easy access to mono-functionalized geometrically varied poly-(guanidinium)amines. The new building blocks were introduced into cationic lipids to obtain novel poly-(guanidinium)amine lipids, which are potential DNA vectors for gene delivery. Copyright 1998 John Wiley & Sons, Inc.

  15. Identification of nitrogen-fixing genes and gene clusters from metagenomic library of acid mine drainage.

    Science.gov (United States)

    Dai, Zhimin; Guo, Xue; Yin, Huaqun; Liang, Yili; Cong, Jing; Liu, Xueduan

    2014-01-01

    Biological nitrogen fixation is an essential function of acid mine drainage (AMD) microbial communities. However, most acidophiles in AMD environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in AMD microbial communities. In this study, we used metagenomic sequencing to isolate nif genes in the AMD microbial community from Dexing Copper Mine, China. Meanwhile, a metagenome microarray containing 7,776 large-insertion fosmids was constructed to screen novel nif gene clusters. Metagenomic analyses revealed that 742 sequences were identified as nif genes including structural subunit genes nifH, nifD, nifK and various additional genes. The AMD community is massively dominated by the genus Acidithiobacillus. However, the phylogenetic diversity of nitrogen-fixing microorganisms is much higher than previously thought in the AMD community. Furthermore, a 32.5-kb genomic sequence harboring nif, fix and associated genes was screened by metagenome microarray. Comparative genome analysis indicated that most nif genes in this cluster are most similar to those of Herbaspirillum seropedicae, but the organization of the nif gene cluster had significant differences from H. seropedicae. Sequence analysis and reverse transcription PCR also suggested that distinct transcription units of nif genes exist in this gene cluster. nifQ gene falls into the same transcription unit with fixABCX genes, which have not been reported in other diazotrophs before. All of these results indicated that more novel diazotrophs survive in the AMD community.

  16. Identification of nitrogen-fixing genes and gene clusters from metagenomic library of acid mine drainage.

    Directory of Open Access Journals (Sweden)

    Zhimin Dai

    Full Text Available Biological nitrogen fixation is an essential function of acid mine drainage (AMD microbial communities. However, most acidophiles in AMD environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in AMD microbial communities. In this study, we used metagenomic sequencing to isolate nif genes in the AMD microbial community from Dexing Copper Mine, China. Meanwhile, a metagenome microarray containing 7,776 large-insertion fosmids was constructed to screen novel nif gene clusters. Metagenomic analyses revealed that 742 sequences were identified as nif genes including structural subunit genes nifH, nifD, nifK and various additional genes. The AMD community is massively dominated by the genus Acidithiobacillus. However, the phylogenetic diversity of nitrogen-fixing microorganisms is much higher than previously thought in the AMD community. Furthermore, a 32.5-kb genomic sequence harboring nif, fix and associated genes was screened by metagenome microarray. Comparative genome analysis indicated that most nif genes in this cluster are most similar to those of Herbaspirillum seropedicae, but the organization of the nif gene cluster had significant differences from H. seropedicae. Sequence analysis and reverse transcription PCR also suggested that distinct transcription units of nif genes exist in this gene cluster. nifQ gene falls into the same transcription unit with fixABCX genes, which have not been reported in other diazotrophs before. All of these results indicated that more novel diazotrophs survive in the AMD community.

  17. Identification of Nitrogen-Fixing Genes and Gene Clusters from Metagenomic Library of Acid Mine Drainage

    Science.gov (United States)

    Yin, Huaqun; Liang, Yili; Cong, Jing; Liu, Xueduan

    2014-01-01

    Biological nitrogen fixation is an essential function of acid mine drainage (AMD) microbial communities. However, most acidophiles in AMD environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in AMD microbial communities. In this study, we used metagenomic sequencing to isolate nif genes in the AMD microbial community from Dexing Copper Mine, China. Meanwhile, a metagenome microarray containing 7,776 large-insertion fosmids was constructed to screen novel nif gene clusters. Metagenomic analyses revealed that 742 sequences were identified as nif genes including structural subunit genes nifH, nifD, nifK and various additional genes. The AMD community is massively dominated by the genus Acidithiobacillus. However, the phylogenetic diversity of nitrogen-fixing microorganisms is much higher than previously thought in the AMD community. Furthermore, a 32.5-kb genomic sequence harboring nif, fix and associated genes was screened by metagenome microarray. Comparative genome analysis indicated that most nif genes in this cluster are most similar to those of Herbaspirillum seropedicae, but the organization of the nif gene cluster had significant differences from H. seropedicae. Sequence analysis and reverse transcription PCR also suggested that distinct transcription units of nif genes exist in this gene cluster. nifQ gene falls into the same transcription unit with fixABCX genes, which have not been reported in other diazotrophs before. All of these results indicated that more novel diazotrophs survive in the AMD community. PMID:24498417

  18. Presentation of the selected French libraries and the French library system

    Directory of Open Access Journals (Sweden)

    Moja Kotar

    2007-01-01

    Full Text Available The Selected French libraries (Bibliotheque nationale de France, Bibliotheque publique d’information, multimedia library of the Cité des sciences et de l’industrie as well as Paris public libraries are presented in the article. France does not have a union catalogue at the national level, therefore libraries use different platforms for shared cataloguing and compile more union catalogues. According to their needs, French libraries join into consortia for the acquisition of electronic resources, which can be either geographically or thematically delimited or formed by the institutions of the same status. The author believes that the Slovenian library network works well considering much smaller budget for culture and higher education in comparison with France. To improve its performance, more funds would have to be allocated and higher reputation of the library profession achieved, comparable to the situation in France. Digitization of resources is the area where the Slovenian librarianship lags most behind the French one.

  19. A binary plasmid system for shuffling combinatorial antibody libraries.

    OpenAIRE

    Collet, T A; Roben, P; O'Kennedy, R; Barbas, C F; Burton, D R; Lerner, R A

    1992-01-01

    We have used a binary system of replicon-compatible plasmids to test the potential for promiscuous recombination of heavy and light chains within sets of human Fab fragments isolated from combinatorial antibody libraries. Antibody molecules showed a surprising amount of promiscuity in that a particular heavy chain could recombine with multiple light chains with retention of binding to a protein antigen. The degree to which a given heavy chain productively paired with any light chain to bind a...

  20. Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota

    Directory of Open Access Journals (Sweden)

    Chiara Devirgiliis

    2014-01-01

    Full Text Available Lactic acid bacteria (LAB represent the predominant microbiota in fermented foods. Foodborne LAB have received increasing attention as potential reservoir of antibiotic resistance (AR determinants, which may be horizontally transferred to opportunistic pathogens. We have previously reported isolation of AR LAB from the raw ingredients of a fermented cheese, while AR genes could be detected in the final, marketed product only by PCR amplification, thus pointing at the need for more sensitive microbial isolation techniques. We turned therefore to construction of a metagenomic library containing microbial DNA extracted directly from the food matrix. To maximize yield and purity and to ensure that genomic complexity of the library was representative of the original bacterial population, we defined a suitable protocol for total DNA extraction from cheese which can also be applied to other lipid-rich foods. Functional library screening on different antibiotics allowed recovery of ampicillin and kanamycin resistant clones originating from Streptococcus salivarius subsp. thermophilus and Lactobacillus helveticus genomes. We report molecular characterization of the cloned inserts, which were fully sequenced and shown to confer AR phenotype to recipient bacteria. We also show that metagenomics can be applied to food microbiota to identify underrepresented species carrying specific genes of interest.

  1. Synthetic sustained gene delivery systems.

    Science.gov (United States)

    Agarwal, Ankit; Mallapragada, Surya K

    2008-01-01

    Gene therapy today is hampered by the need of a safe and efficient gene delivery system that can provide a sustained therapeutic effect without cytotoxicity or unwanted immune responses. Bolus gene delivery in solution results in the loss of delivered factors via lymphatic system and may cause undesired effects by the escape of bioactive molecules to distant sites. Controlled gene delivery systems, acting as localized depot of genes, provide an extended sustained release of genes, giving prolonged maintenance of the therapeutic level of encoded proteins. They also limit the DNA degradation in the nuclease rich extra-cellular environment. While attempts have been made to adapt existing controlled drug delivery technologies, more novel approaches are being investigated for controlled gene delivery. DNA encapsulated in nano/micro spheres of polymers have been administered systemically/orally to be taken up by the targeted tissues and provide sustained release once internalized. Alternatively, DNA entrapped in hydrogels or scaffolds have been injected/implanted in tissues/cavities as platforms for gene delivery. The present review examines these different modalities for sustained delivery of viral and non-viral gene-delivery vectors. Design parameters and release mechanisms of different systems made with synthetic or natural polymers are presented along with their prospective applications and opportunities for continuous development.

  2. Systematic hybrid LOH: a new method to reduce false positives and negatives during screening of yeast gene deletion libraries

    DEFF Research Database (Denmark)

    Alvaro, D.; Sunjevaric, I.; Reid, R. J.

    2006-01-01

    We have developed a new method, systematic hybrid loss of heterozygosity, to facilitate genomic screens utilizing the yeast gene deletion library. Screening is performed using hybrid diploid strains produced through mating the library haploids with strains from a different genetic background......, to minimize the contribution of unpredicted recessive genetic factors present in the individual library strains. We utilize a set of strains where each contains a conditional centromere construct on one of the 16 yeast chromosomes that allows the destabilization and selectable loss of that chromosome. After...... complementation of any spurious recessive mutations in the library strain, facilitating attribution of the observed phenotype to the documented gene deletion and dramatically reducing false positive results commonly obtained in library screens. The systematic hybrid LOH method can be applied to virtually any...

  3. The Digital Library for Earth System Education: A Community Integrator

    Science.gov (United States)

    Marlino, M. R.; Pandya, R. E.

    2003-12-01

    The rapid changes in the geoscience research environment have prompted educators to request support for their efforts to reform geoscience educational practices. DLESE, the Digital Library for Earth System Education, responds to this request by providing a single point of access to high-quality educational resources for teaching about the Earth as a system. DLESE is supported by the National Science Foundation and is an operational library used by tens of thousands of educators every month. DLESE resources include a variety of media formats, from text-based lesson plans to highly-sophisticated tools for interactive three-dimensional visualization of authentic scientific data. The DLESE community is particularly interested in partnering with scientific researchers to ensure that the tools of practicing scientists become widely available to geoscience educators. Two emerging large-scale scientific efforts, the GEON project and EarthScope, provide compelling illustrations of the potential of these partnerships. Both are cutting-edge, cross-disciplinary projects that use digital tools in a distributed environment to support scientific investigation. Both have also made a deep commitment to use these same tools to support geoscience education, and both are including DLESE as part of that commitment. Our interactive presentation will allow users to discover a variety of educational resources and communication services within the library. We will highlight those library resources and services that take particular advantage of the digital media to support new modes of learning and teaching. For example, annotation tools allow educators to add tips on the most effective way to use a specific resource. Data services will help educators find and use real-time data to illustrate geoscience phenomena. Multi-dimensional visualization tools allow students to interact with authentic student data in inquiry-based learning environment. DLESE will continue to actively collaborate

  4. Construction of a BAC library and identification of Dmrt1 gene of the rice field eel, Monopterus albus

    International Nuclear Information System (INIS)

    Jang Songhun; Zhou Fang; Xia Laixin; Zhao Wei; Cheng Hanhua; Zhou Rongjia

    2006-01-01

    A bacterial artificial chromosome (BAC) library was constructed using nuclear DNA from the rice field eel (Monopterus albus). The BAC library consists of a total of 33,000 clones with an average insert size of 115 kb. Based on the rice field eel haploid genome size of 600 Mb, the BAC library is estimated to contain approximately 6.3 genome equivalents and represents 99.8% of the genome of the rice field eel. This is first BAC library constructed from this species. To estimate the possibility of isolating a specific clone, high-density colony hybridization-based library screening was performed using Dmrt1 cDNA of the rice field eel as a probe. Both library screening and PCR identification results revealed three positive BAC clones which were overlapped, and formed a contig covering the Dmrt1 gene of 195 kb. By sequence comparisons with the Dmrt1 cDNA and sequencing of first four intron-exon junctions, Dmrt1 gene of the rice field eel was predicted to contain four introns and five exons. The sizes of first and second intron are 1.5 and 2.6 kb, respectively, and the sizes of last two introns were predicted to be about 20 kb. The Dmrt1 gene structure was conserved in evolution. These results also indicate that the BAC library is a useful resource for BAC contig construction and molecular isolation of functional genes

  5. Methodology optimizing SAGE library tag-to-gene mapping: application to Leishmania

    Directory of Open Access Journals (Sweden)

    Smandi Sondos

    2012-01-01

    Full Text Available Abstract Background Leishmaniasis are widespread parasitic-diseases with an urgent need for more active and less toxic drugs and for effective vaccines. Understanding the biology of the parasite especially in the context of host parasite interaction is a crucial step towards such improvements in therapy and control. Several experimental approaches including SAGE (Serial analysis of gene expression have been developed in order to investigate the parasite transcriptome organisation and plasticity. Usual SAGE tag-to-gene mapping techniques are inadequate because almost all tags are normally located in the 3'-UTR outside the CDS, whereas most information available for Leishmania transcripts is restricted to the CDS predictions. The aim of this work is to optimize a SAGE libraries tag-to-gene mapping technique and to show how this development improves the understanding of Leishmania transcriptome. Findings The in silico method implemented herein was based on mapping the tags to Leishmania genome using BLAST then mapping the tags to their gene using a data-driven probability distribution. This optimized tag-to-gene mappings improved the knowledge of Leishmania genome structure and transcription. It allowed analyzing the expression of a maximal number of Leishmania genes, the delimitation of the 3' UTR of 478 genes and the identification of biological processes that are differentially modulated during the promastigote to amastigote differentiation. Conclusion The developed method optimizes the assignment of SAGE tags in trypanosomatidae genomes as well as in any genome having polycistronic transcription and small intergenic regions.

  6. An Integrated Library System on the CERN Document Server

    CERN Document Server

    Rodrigues Silvestre, Joaquim Jorge; Le Meur, Jean-Yves; Šimko, Tibor

    2010-01-01

    CERN – The European Organization for Nuclear Research – is one of the largest research centres worldwide, responsible for several discoveries in physics as well as in computer science. The CERN Document Server, also known as CDS Invenio, is a software developed at CERN, which aims to provide a set of tools for managing digital libraries. In order to improve the functionalities of CDS Invenio a new module was developed , called BibCirculation, to manage books (and other items) from the CERN library, and working as an Integrated Library System. This thesis shows the steps that have been done to achieve the several goals of this project, explaining, among others aspects, the process of integration with other existing modules as well as the way to associate the information about books with the metadata from CDS Invenio. You can also find a detailed explanation of the entire implementation process and testing. Finally, there are presented the conclusions of this project and ideas for future development.

  7. A computerized energy systems code and information library at Soreq

    Energy Technology Data Exchange (ETDEWEB)

    Silverman, I; Shapira, M; Caner, D; Sapier, D [Israel Atomic Energy Commission, Yavne (Israel). Soreq Nuclear Research Center

    1996-12-01

    In the framework of the contractual agreement between the Ministry of Energy and Infrastructure and the Division of Nuclear Engineering of the Israel Atomic Energy Commission, both Soreq-NRC and Ben-Gurion University have agreed to establish, in 1991, a code center. This code center contains a library of computer codes and relevant data, with particular emphasis on nuclear power plant research and development support. The code center maintains existing computer codes and adapts them to the ever changing computing environment, keeps track of new code developments in the field of nuclear engineering, and acquires the most recent revisions of computer codes of interest. An attempt is made to collect relevant codes developed in Israel and to assure that proper documentation and application instructions are available. En addition to computer programs, the code center collects sample problems and international benchmarks to verify the codes and their applications to various areas of interest to nuclear power plant engineering and safety evaluation. Recently, the reactor simulation group at Soreq acquired, using funds provided by the Ministry of Energy and Infrastructure, a PC work station operating under a Linux operating system to give users of the library an easy on-line way to access resources available at the library. These resources include the computer codes and their documentation, reports published by the reactor simulation group, and other information databases available at Soreq. Registered users set a communication line, through a modem, between their computer and the new workstation at Soreq and use it to download codes and/or information or to solve their problems, using codes from the library, on the computer at Soreq (authors).

  8. A computerized energy systems code and information library at Soreq

    International Nuclear Information System (INIS)

    Silverman, I.; Shapira, M.; Caner, D.; Sapier, D.

    1996-01-01

    In the framework of the contractual agreement between the Ministry of Energy and Infrastructure and the Division of Nuclear Engineering of the Israel Atomic Energy Commission, both Soreq-NRC and Ben-Gurion University have agreed to establish, in 1991, a code center. This code center contains a library of computer codes and relevant data, with particular emphasis on nuclear power plant research and development support. The code center maintains existing computer codes and adapts them to the ever changing computing environment, keeps track of new code developments in the field of nuclear engineering, and acquires the most recent revisions of computer codes of interest. An attempt is made to collect relevant codes developed in Israel and to assure that proper documentation and application instructions are available. En addition to computer programs, the code center collects sample problems and international benchmarks to verify the codes and their applications to various areas of interest to nuclear power plant engineering and safety evaluation. Recently, the reactor simulation group at Soreq acquired, using funds provided by the Ministry of Energy and Infrastructure, a PC work station operating under a Linux operating system to give users of the library an easy on-line way to access resources available at the library. These resources include the computer codes and their documentation, reports published by the reactor simulation group, and other information databases available at Soreq. Registered users set a communication line, through a modem, between their computer and the new workstation at Soreq and use it to download codes and/or information or to solve their problems, using codes from the library, on the computer at Soreq (authors)

  9. Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality.

    Science.gov (United States)

    Liberati, Nicole T; Urbach, Jonathan M; Thurber, Tara K; Wu, Gang; Ausubel, Frederick M

    2008-01-01

    Putative essential genes can be identified by comparing orthologs not disrupted in multiple near-saturated transposon insertion mutation libraries in related strains of the same bacterial species. Methods for identifying all orthologs between two bacterial strains and putative essential orthologs are described. In addition, protocols detailing near-saturation transposon insertion mutagenesis of bacteria are presented, including (1) conjugation-mediated mutagenesis, (2) automated colony picking and liquid handling of mutant cultures, and (3) arbitrary polymerase chain reaction amplification and sequencing of genomic DNA adjacent to transposon insertion sites.

  10. Transgenic Arabidopsis Gene Expression System

    Science.gov (United States)

    Ferl, Robert; Paul, Anna-Lisa

    2009-01-01

    The Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors (an organism used to determine the quality of the surrounding environment) of their environment using real-time nondestructive Green Fluorescent Protein (GFP) imagery and traditional postflight analyses.

  11. Performance comparison analysis library communication cluster system using merge sort

    Science.gov (United States)

    Wulandari, D. A. R.; Ramadhan, M. E.

    2018-04-01

    Begins by using a single processor, to increase the speed of computing time, the use of multi-processor was introduced. The second paradigm is known as parallel computing, example cluster. The cluster must have the communication potocol for processing, one of it is message passing Interface (MPI). MPI have many library, both of them OPENMPI and MPICH2. Performance of the cluster machine depend on suitable between performance characters of library communication and characters of the problem so this study aims to analyze the comparative performances libraries in handling parallel computing process. The case study in this research are MPICH2 and OpenMPI. This case research execute sorting’s problem to know the performance of cluster system. The sorting problem use mergesort method. The research method is by implementing OpenMPI and MPICH2 on a Linux-based cluster by using five computer virtual then analyze the performance of the system by different scenario tests and three parameters for to know the performance of MPICH2 and OpenMPI. These performances are execution time, speedup and efficiency. The results of this study showed that the addition of each data size makes OpenMPI and MPICH2 have an average speed-up and efficiency tend to increase but at a large data size decreases. increased data size doesn’t necessarily increased speed up and efficiency but only execution time example in 100000 data size. OpenMPI has a execution time greater than MPICH2 example in 1000 data size average execution time with MPICH2 is 0,009721 and OpenMPI is 0,003895 OpenMPI can customize communication needs.

  12. Hacking Blackboard: Customizing Access to Library Resources through the Blackboard Course Management System

    Science.gov (United States)

    Kellam, Lynda M.; Cox, Richard; Winkler, Hannah

    2009-01-01

    Academic libraries have long been trying to gain access to users through their favorite online spaces, such as social networking sites. In this article a project of the University of North Carolina at Greensboro's University Libraries, which integrated library resources with the campus course management system, Blackboard, is detailed. The main…

  13. Generation and tests of the new version 88 library for HAMMER system

    International Nuclear Information System (INIS)

    Chalhoub, E.S.; Anaf, J.

    1988-08-01

    A new library, version 88, for the HAMMER system, was generated using the ENDF/B-IV FRENDL evaluated nuclear data libraries. It is composed of epithermal and thermal libraries obtained by processing the new versions of ETOG-3 and FLANGE-II codes, respectively. Test results for two benchmark critical assemblies are presented. (author) [pt

  14. Semantically Enriching the Search System of a Music Digital Library

    Science.gov (United States)

    de Juan, Paloma; Iglesias, Carlos

    Traditional search systems are usually based on keywords, a very simple and convenient mechanism to express a need for information. This is the most popular way of searching the Web, although it is not always an easy task to accurately summarize a natural language query in a few keywords. Working with keywords means losing the context, which is the only thing that can help us deal with ambiguity. This is the biggest problem of keyword-based systems. Semantic Web technologies seem a perfect solution to this problem, since they make it possible to represent the semantics of a given domain. In this chapter, we present three projects, Harmos, Semusici and Cantiga, whose aim is to provide access to a music digital library. We will describe two search systems, a traditional one and a semantic one, developed in the context of these projects and compare them in terms of usability and effectiveness.

  15. School Library Supplement. Putting Your Library Automation System to Work [and] Using Videodiscs in Research Projects [and] Library Security: What to Look For [and] Expanding Library/Media Services with Media Retrieval [and] How to Network CD-ROMs (and) The School Library Resource Directory.

    Science.gov (United States)

    Karpisek, Marian; And Others

    1995-01-01

    Presents five articles and a company resource directory to help librarians successfully incorporate technology into school libraries. Discusses actual situations, examines student needs, and gives advice to help librarians with library automation systems, videodiscs, library security systems, media retrieval, networking CD-ROMs, and locating…

  16. A binary plasmid system for shuffling combinatorial antibody libraries.

    Science.gov (United States)

    Collet, T A; Roben, P; O'Kennedy, R; Barbas, C F; Burton, D R; Lerner, R A

    1992-11-01

    We have used a binary system of replicon-compatible plasmids to test the potential for promiscuous recombination of heavy and light chains within sets of human Fab fragments isolated from combinatorial antibody libraries. Antibody molecules showed a surprising amount of promiscuity in that a particular heavy chain could recombine with multiple light chains with retention of binding to a protein antigen. The degree to which a given heavy chain productively paired with any light chain to bind antigen varied from 43% to 100% and depended strongly on the heavy-chain sequence. Such productive crosses resulted in a set of Fab fragments of similar apparent binding constants, which seemed to differ mainly in the amount of active Fab fragment produced in the bacterial cell. The dominance of the heavy chain in the antibody-antigen interaction was further explored in a set of directed crosses, in which heavy and light chains derived from antigen-specific clones were crossed with nonrelated heavy and light chains. In these crosses, an Fab fragment retained antigen binding only if it contained a heavy chain from an antigen-specific clone. In no case did the light chain confer detectable affinity when paired with indifferent heavy chains. The surprising promiscuity of heavy chains has ramifications for the evaluation of the diversity of combinatorial libraries made against protein antigens and should allow the combination of one such promiscuous heavy chain with an engineered light chain to form an Fab fragment carrying synthetic cofactors to assist in antibody catalysis.

  17. Construction of C35 gene bait recombinants and T47D cell cDNA library.

    Science.gov (United States)

    Yin, Kun; Xu, Chao; Zhao, Gui-Hua; Liu, Ye; Xiao, Ting; Zhu, Song; Yan, Ge

    2017-11-20

    C35 is a novel tumor biomarker associated with metastasis progression. To investigate the interaction factors of C35 in its high expressed breast cancer cell lines, we constructed bait recombinant plasmids of C35 gene and T47D cell cDNA library for yeast two-hybrid screening. Full length C35 sequences were subcloned using RT-PCR from cDNA template extracted from T47D cells. Based on functional domain analysis, the full-length C35 1-348bp was also truncated into two fragments C351-153bp and C35154-348bp to avoid auto-activation. The three kinds of C35 genes were successfully amplified and inserted into pGBKT7 to construct bait recombinant plasmids pGBKT7-C351-348bp, pGBKT7-C351-153bp and pGBKT7-C35154-348bp, then transformed into Y187 yeast cells by the lithium acetate method. Auto-activation and toxicity of C35 baits were detected using nutritional deficient medium and X-α-Gal assays. The T47D cell ds cDNA was generated by SMART TM technology and the library was constructed using in vivo recombination-mediated cloning in the AH109 yeast strain using a pGADT7-Rec plasmid. The transformed Y187/pGBKT7-C351-348bp line was intensively inhibited while the truncated Y187/pGBKT7-C35 lines had no auto-activation and toxicity in yeast cells. The titer of established cDNA library was 2 × 10 7 pfu/mL with high transformation efficiency of 1.4 × 10 6 , and the insert size of ds cDNA was distributed homogeneously between 0.5-2.0 kb. Our research generated a T47D cell cDNA library with high titer, and the constructed two C35 "baits" contained a respective functional immunoreceptor tyrosine based activation motif (ITAM) and the conserved last four amino acids Cys-Ile-Leu-Val (CILV) motif, and therefore laid a foundation for screening the C35 interaction factors in a BC cell line.

  18. From the Cover: A polymer library approach to suicide gene therapy for cancer

    Science.gov (United States)

    Anderson, Daniel G.; Peng, Weidan; Akinc, Akin; Hossain, Naushad; Kohn, Anat; Padera, Robert; Langer, Robert; Sawicki, Janet A.

    2004-11-01

    Optimal gene therapy for cancer must (i) deliver DNA to tumor cells with high efficiency, (ii) induce minimal toxicity, and (iii) avoid gene expression in healthy tissues. To this end, we generated a library of >500 degradable, poly(-amino esters) for potential use as nonviral DNA vectors. Using high-throughput methods, we screened this library in vitro for transfection efficiency and cytotoxicity. We tested the best performing polymer, C32, in mice for toxicity and DNA delivery after intratumor and i.m. injection. C32 delivered DNA intratumorally 4-fold better than one of the best commercially available reagents, jetPEI (polyethyleneimine), and 26-fold better than naked DNA. Conversely, the highest transfection levels after i.m. administration were achieved with naked DNA, followed by polyethyleneimine; transfection was rarely observed with C32. Additionally, polyethyleneimine induced significant local toxicity after i.m. injection, whereas C32 demonstrated no toxicity. Finally, we used C32 to deliver a DNA construct encoding the A chain of diphtheria toxin (DT-A) to xenografts derived from LNCaP human prostate cancer cells. This construct regulates toxin expression both at the transcriptional level by the use of a chimeric-modified enhancer/promoter sequence of the human prostate-specific antigen gene and by DNA recombination mediated by Flp recombinase. C32 delivery of the A chain of diphtheria toxin DNA to LNCaP xenografts suppressed tumor growth and even caused 40% of tumors to regress in size. Because C32 transfects tumors locally at high levels, transfects healthy muscle poorly, and displays no toxicity, it may provide a vehicle for the local treatment of cancer. prostate | cationic polymers

  19. Chitinase genes revealed and compared in bacterial isolates, DNA extracts and a metagenomic library from a phytopathogen suppressive soil

    Energy Technology Data Exchange (ETDEWEB)

    Hjort, K.; Bergstrom, M.; Adesina, M.F.; Jansson, J.K.; Smalla, K.; Sjoling, S.

    2009-09-01

    Soil that is suppressive to disease caused by fungal pathogens is an interesting source to target for novel chitinases that might be contributing towards disease suppression. In this study we screened for chitinase genes, in a phytopathogen-suppressive soil in three ways: (1) from a metagenomic library constructed from microbial cells extracted from soil, (2) from directly extracted DNA and (3) from bacterial isolates with antifungal and chitinase activities. Terminal-restriction fragment length polymorphism (T-RFLP) of chitinase genes revealed differences in amplified chitinase genes from the metagenomic library and the directly extracted DNA, but approximately 40% of the identified chitinase terminal-restriction fragments (TRFs) were found in both sources. All of the chitinase TRFs from the isolates were matched to TRFs in the directly extracted DNA and the metagenomic library. The most abundant chitinase TRF in the soil DNA and the metagenomic library corresponded to the TRF{sup 103} of the isolate, Streptomyces mutomycini and/or Streptomyces clavifer. There were good matches between T-RFLP profiles of chitinase gene fragments obtained from different sources of DNA. However, there were also differences in both the chitinase and the 16S rRNA gene T-RFLP patterns depending on the source of DNA, emphasizing the lack of complete coverage of the gene diversity by any of the approaches used.

  20. Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery.

    Science.gov (United States)

    Kiljunen, Saija; Pajunen, Maria I; Dilks, Kieran; Storf, Stefanie; Pohlschroder, Mechthild; Savilahti, Harri

    2014-12-09

    Archaea share fundamental properties with bacteria and eukaryotes. Yet, they also possess unique attributes, which largely remain poorly characterized. Haloferax volcanii is an aerobic, moderately halophilic archaeon that can be grown in defined media. It serves as an excellent archaeal model organism to study the molecular mechanisms of biological processes and cellular responses to changes in the environment. Studies on haloarchaea have been impeded by the lack of efficient genetic screens that would facilitate the identification of protein functions and respective metabolic pathways. Here, we devised an insertion mutagenesis strategy that combined Mu in vitro DNA transposition and homologous-recombination-based gene targeting in H. volcanii. We generated an insertion mutant library, in which the clones contained a single genomic insertion. From the library, we isolated pigmentation-defective and auxotrophic mutants, and the respective insertions pinpointed a number of genes previously known to be involved in carotenoid and amino acid biosynthesis pathways, thus validating the performance of the methodologies used. We also identified mutants that had a transposon insertion in a gene encoding a protein of unknown or putative function, demonstrating that novel roles for non-annotated genes could be assigned. We have generated, for the first time, a random genomic insertion mutant library for a halophilic archaeon and used it for efficient gene discovery. The library will facilitate the identification of non-essential genes behind any specific biochemical pathway. It represents a significant step towards achieving a more complete understanding of the unique characteristics of halophilic archaea.

  1. Description of Transmutation Library for Fuel Cycle System Analyses

    International Nuclear Information System (INIS)

    Piet, Steven J.; Bays, Samuel E.; Hoffman, Edward A.

    2010-01-01

    This report documents the Transmutation Library that is used in Fuel Cycle System Analyses. This version replaces the 2008 version.(Piet2008) The Transmutation Library has the following objectives: (1) Assemble past and future transmutation cases for system analyses. (2) For each case, assemble descriptive information such as where the case was documented, the purpose of the calculation, the codes used, source of feed material, transmutation parameters, and the name of files that contain raw or source data. (3) Group chemical elements so that masses in separation and waste processes as calculated in dynamic simulations or spreadsheets reflect current thinking of those processes. For example, the CsSr waste form option actually includes all Group 1A and 2A elements. (4) Provide mass fractions at input (charge) and output (discharge) for each case. (5) Eliminate the need for either ''fission product other'' or ''actinide other'' while conserving mass. Assessments of waste and separation cannot use ''fission product other'' or ''actinide other'' as their chemical behavior is undefined. (6) Catalog other isotope-specific information in one place, e.g., heat and dose conversion factors for individual isotopes. (7) Describe the correlations for how input and output compositions change as a function of UOX burnup (for LWR UOX fuel) or fast reactor (FR) transuranic (TRU) conversion ratio (CR) for either FR-metal or FR-oxide. This document therefore includes the following sections: (1) Explanation of the data set information, i.e., the data that describes each case. In no case are all of the data presented in the Library included in previous documents. In assembling the Library, we return to raw data files to extract the case and isotopic data, into the specified format. (2) Explanation of which isotopes and elements are tracked. For example, the transition metals are tracked via the following: two Zr isotopes, Zr-other, Tc99, Tc-other, two Mo-Ru-Rh-Pd isotopes, Mo

  2. Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction.

    Science.gov (United States)

    Li, Aitao; Acevedo-Rocha, Carlos G; Sun, Zhoutong; Cox, Tony; Xu, Jia Lucy; Reetz, Manfred T

    2018-02-02

    Saturation mutagenesis (SM) constitutes a widely used technique in the directed evolution of selective enzymes as catalysts in organic chemistry and in the manipulation of metabolic paths and genomes, but the quality of the libraries is far from optimal due to the inherent amino acid bias. Herein, it is shown how this fundamental problem can be solved by applying high-fidelity solid-phase chemical gene synthesis on silicon chips followed by efficient gene assembly. Limonene epoxide hydrolase was chosen as the catalyst in the model desymmetrization of cyclohexene oxide with the stereoselective formation of (R,R)- and (S,S)-cyclohexane-1,2-diol. A traditional combinatorial PCR-based SM library, produced by simultaneous randomization at several residues by using a reduced amino acid alphabet, and the respective synthetic library were constructed and compared. Statistical analysis at the DNA level with massive sequencing demonstrates that, in the synthetic approach, 97 % of the theoretically possible DNA mutants are formed, whereas the traditional SM library contained only about 50 %. Screening at the protein level also showed the superiority of the synthetic library; many highly (R,R)- and (S,S)-selective variants being discovered are not found in the traditional SM library. With the prices of synthetic genes decreasing, this approach may point the way to future directed evolution. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Construction and characterization of a forward subtracted library of blue mussels Mytilus edulis for the identification of gene transcription signatures and biomarkers of styrene exposure

    International Nuclear Information System (INIS)

    Diaz de Cerio, O.; Hands, E.; Humble, J.; Cajaraville, M.P.; Craft, J.A.; Cancio, I.

    2013-01-01

    Highlights: ► Transcription responses in blue mussels exposed to styrene have been studied by SSH. ► 1440 Clones were obtained from which 287 were sequenced. ► Immune system, cancer-related and ribosomal genes identified as upregulated genes. ► Chitin and β-1-3-glucan metabolism genes highly represented in subtracted library. -- Abstract: Transcriptional profiling can elucidate adaptive/toxicity pathways participating in achieving homeostasis or leading to pathogenesis in marine biota exposed to chemical substances. With the aim of analyzing transcriptional responses in the mussel Mytilus edulis exposed to the corrosive and putatively carcinogenic hydrocarbon styrene (3–5 ppm, 3 days), a forward subtracted (SSH) cDNA library was produced. Female mussels were selected and digestive gland mRNA was isolated. A library with 1440 clones was produced and a total of 287 clones were sequenced, 53% being identified through BlastN analysis against Mytibase and DeepSeaVent databases. Those genes included GO terms such as ‘response to drugs’, ‘immune defense’ and ‘cell proliferation’. Furthermore, sequences related to chitin and beta-1-3-glucan metabolism were also up-regulated by styrene. Many of the obtained sequences could not be annotated constituting new mussel sequences. In conclusion, this SSH study reveals novel sequences useful to generate molecular biomarkers of styrene exposure in mussels

  4. Performance Improvement with Web Based Database on Library Information System of Smk Yadika 5

    Directory of Open Access Journals (Sweden)

    Pualam Dipa Nusantara

    2015-12-01

    Full Text Available The difficulty in managing the data of books collection in the library is a problem that is often faced by the librarian that effect the quality of service. Arrangement and recording a collection of books in the file system of separate applications Word and Excel, as well as transaction handling borrowing and returning books, therehas been no integrated records. Library system can manage the book collection. This system can reduce the problems often experienced by library staff when serving students in borrowing books. There so frequent difficulty in managing the books that still in borrowed state. This system will also record a collection of late fees or lost library conducted by students (borrowers. The conclusion of this study is library performance can be better with the library system using web database.

  5. MpTheory Java library: a multi-platform Java library for systems biology based on the Metabolic P theory.

    Science.gov (United States)

    Marchetti, Luca; Manca, Vincenzo

    2015-04-15

    MpTheory Java library is an open-source project collecting a set of objects and algorithms for modeling observed dynamics by means of the Metabolic P (MP) theory, that is, a mathematical theory introduced in 2004 for modeling biological dynamics. By means of the library, it is possible to model biological systems both at continuous and at discrete time. Moreover, the library comprises a set of regression algorithms for inferring MP models starting from time series of observations. To enhance the modeling experience, beside a pure Java usage, the library can be directly used within the most popular computing environments, such as MATLAB, GNU Octave, Mathematica and R. The library is open-source and licensed under the GNU Lesser General Public License (LGPL) Version 3.0. Source code, binaries and complete documentation are available at http://mptheory.scienze.univr.it. luca.marchetti@univr.it, marchetti@cosbi.eu Supplementary data are available at Bioinformatics online. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  6. The Energy Science and Technology Database on a local library system: A case study at the Los Alamos National Research Library

    Energy Technology Data Exchange (ETDEWEB)

    Holtkamp, I.S.

    1994-10-01

    This paper presents an overview of efforts at Los Alamos National Laboratory to acquire and mount the Energy Science and Technology Database (EDB) as a citation database on the Research Library`s Geac Advance system. The rationale for undertaking this project and expected benefits are explained. Significant issues explored are loading non-USMARC records into a MARC-based library system, the use of EDB records to replace or supplement in-house cataloging of technical reports, the impact of different cataloging standards and database size on searching and retrieval, and how integrating an external database into the library`s online catalog may affect staffing and workflow.

  7. Block copolymer libraries: modular versatility of the macromolecular Lego system.

    Science.gov (United States)

    Lohmeijer, Bas G G; Wouters, Daan; Yin, Zhihui; Schubert, Ulrich S

    2004-12-21

    The synthesis and characterization of a new 4 x 4 library of block copolymers based on polystyrene and poly(ethylene oxide) connected by an asymmetrical octahedral bis(terpyridine) ruthenium complex at the block junction are described, while initial studies on the thin film morphology of the components of the library are presented by the use of Atomic Force Microscopy, demonstrating the impact of a library approach to derive structure-property relationships.

  8. Construction of an Americn mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

    DEFF Research Database (Denmark)

    Anistoroaei, Razvan Marian; Hallers, Boudewijn ten; Nefedov, Michael

    2011-01-01

    BACKGROUND: Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. RESULTS: Here, we...... report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison). The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each...... consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs), representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library...

  9. Libraries on the MOVE.

    Science.gov (United States)

    Edgar, Jim; And Others

    1986-01-01

    Presents papers from Illinois State Library and Shawnee Library System's "Libraries on the MOVE" conference focusing on how libraries can impact economic/cultural climate of an area. Topics addressed included information services of rural libraries; marketing; rural library development; library law; information access; interagency…

  10. Recent Developments of the Modelica"Buildings" Library for Building Energy and Control Systems

    Energy Technology Data Exchange (ETDEWEB)

    Wetter, Michael; Zuo, Wangda; Nouidui, Thierry Stephane

    2011-04-01

    At the Modelica 2009 conference, we introduced the Buildings library, a freely available Modelica library for building energy and control systems. This paper reports the updates of the library and presents example applications for a range of heating, ventilation and air conditioning (HVAC) systems. Over the past two years, the library has been further developed. The number of HVAC components models has been doubled and various components have been revised to increase numerical robustness.The paper starts with an overview of the library architecture and a description of the main packages. To demonstrate the features of the Buildings library, applications that include multizone airflow simulation as well as supervisory and local loop control of a variable air volume (VAV) system are briefly described. The paper closes with a discussion of the current development.

  11. Construction of an American mink Bacterial Artificial Chromosome (BAC library and sequencing candidate genes important for the fur industry

    Directory of Open Access Journals (Sweden)

    Christensen Knud

    2011-07-01

    Full Text Available Abstract Background Bacterial artificial chromosome (BAC libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the construction and characterization of the CHORI-231 BAC library constructed from a Danish-farmed, male American mink (Neovison vison. The library contains approximately 165,888 clones with an average insert size of 170 kb, representing approximately 10-fold coverage. High-density filters, each consisting of 18,432 clones spotted in duplicate, have been produced for hybridization screening and are publicly available. Overgo probes derived from expressed sequence tags (ESTs, representing 21 candidate genes for traits important for the mink industry, were used to screen the BAC library. These included candidate genes for coat coloring, hair growth and length, coarseness, and some receptors potentially involved in viral diseases in mink. The extensive screening yielded positive results for 19 of these genes. Thirty-five clones corresponding to 19 genes were sequenced using 454 Roche, and large contigs (184 kb in average were assembled. Knowing the complete sequences of these candidate genes will enable confirmation of the association with a phenotype and the finding of causative mutations for the targeted phenotypes. Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80% of BACs were obtained. An excess of 2 Mb has been analyzed, thus giving a snapshot of the mink genome. Conclusions The availability of the CHORI-321 American mink BAC library will aid in identification of genes and genomic regions of interest. We have demonstrated how the library can be used to identify specific genes of interest, develop genetic markers, and for BAC end sequencing and deep sequencing of selected clones. To our knowledge, this is the

  12. Automation at the Fairfax County Virginia Library System.

    Science.gov (United States)

    Baker, Alfred W.; And Others

    The Fairfax County Library converted from a card catalog to a book catalog format in 1963. The first book catalogs were produced by the Sequential Card (SC) process. The master cards were prepared by the library and sent to Science Press, where copy was prepared on IBM cards, coded for sequential filing, and photographed to prepare page plates,…

  13. Interactive Editing and Cataloging Interfaces for Modern Digital Library Systems

    CERN Document Server

    Raae, L C; Helstrup, H

    2009-01-01

    The next-generation High Energy Physics information system, INSPIRE, is being built by combining the content from the successful SPIRES database of bibliographic information with the CDS Invenio software being developed at CERN, an open-source platform for large digital library systems. The project is a collaboration between four major particle physics laboratories in Europe and the U.S. New tools are being developed to enable the global cooperation between catalogers at these labs. The BibEdit module will provide a central interface for the editing, enrichment, correction and verification of a record on its way into the system, by processing and presenting data from several supporting modules to the cataloger. The objective is to minimize the time and actions needed by the cataloger to process the record. To create a fast and powerful web application we make use of modern AJAX technology to create a dynamic and responsive user interface, where server communication happens in the background without delaying t...

  14. Changes in the Composition of Drinking Water Bacterial Clone Libraries Introduced by Using Two Different 16S rRNA Gene PCR Primers

    Science.gov (United States)

    Sequence analysis of 16S rRNA gene clone libraries is a popular tool used to describe the composition of natural microbial communities. Commonly, clone libraries are developed by direct cloning of 16S rRNA gene PCR products. Different primers are often employed in the initial amp...

  15. Linking a Medical User Survey to Management for Library Effectiveness: II, A Checkland Soft Systems Study.

    Science.gov (United States)

    Brember, V. L.

    1985-01-01

    Presents Checkland's soft systems methodology, discusses it in terms of the systems approach, and illustrates how it was used to relate evidence of user survey to practical problems of library management. Difficulties in using methodology are described and implications for library management and information science research are presented. (8…

  16. The Integrated Library System of the 1990s: The OhioLINK Experience.

    Science.gov (United States)

    Hawks, Carol Pitts

    1992-01-01

    Discussion of integrated library systems focuses on the development of the Ohio Library and Information Network (OhioLINK). Capabilities of eight existing systems are described, including catalog creation and maintenance; the online public access catalog (OPAC); circulation, interlibrary loan, and document delivery; acquisitions and serials…

  17. Cross-section library and processing techniques within the SCALE system

    International Nuclear Information System (INIS)

    Westfall, R.M.

    1986-01-01

    A summary of each of the SCALE system features involved in problem-dependent cross section processing is presented. These features include criticality libraries, shielding libraries, the Standard Composition Library, the SCALE functional modules: BONAMI-S, NITAWL-S, XSDRNPM-S, ICE-S, and the Material Information Processor. The automated procedure for cross-section processing is described with examples. 15 refs

  18. Polybacterial community analysis in human conjunctiva through 16S rRNA gene libraries.

    Science.gov (United States)

    Deepthi, KrishnanNair Geetha; Jayasudha, Rajagopalaboopathi; Girish, Rameshan Nair; Manikandan, Palanisamy; Ram, Rammohan; Narendran, Venkatapathy; Prabagaran, Solai Ramatchandirane

    2018-05-14

    The conjunctival sac of healthy human harbours a variety of microorganisms. When the eye is compromised, an occasional inadvertent spread happens to the adjacent tissue, resulting in bacterial ocular infections. Microbiological investigation of the conjunctival swab is one of the broadly used modality to study the aetiological agent of conjunctiva. However, most of the time such methods yield unsatisfactory results. Hence, the present study intends to identify the bacterial community in human conjunctiva of pre-operative subjects through 16S rRNA gene libraries. Out of 45 samples collected from preoperative patients undergoing cataract surgery, 36 libraries were constructed with bacterial nested-PCR-positive samples. The representative clones with unique restriction pattern were generated through Amplified Ribosomal DNA Restriction Analysis (ARDRA) which were sequenced for phylogenetic affiliation. A total of 211 representative clones were obtained which were distributed in phyla Actinobacteria, Firmicutes, α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacteroidetes, and Deinococcus-Thermus. Findings revealed the presence of polybacterial community, especially in some cases even though no bacterium or a single bacterium alone was identified through cultivable method. Remarkably, we identified 17 species which have never been reported in any ocular infections. The sequencing data reported 6 unidentified bacteria suggesting the possibility of novel organisms in the sample. Since, polybacterial community has been identified consisting of both gram positive and gram negative bacteria, a broad spectrum antibiotic therapy is advisable to the patients who are undergoing cataract surgery. Consolidated effort would significantly improve a clear understanding of the nature of microbial community in the human conjunctiva which will promote administration of appropriate antibiotic regimen and also help in the development of oligonucleotide probes to screen the

  19. enhanced digital library system that supports sustainable knowledge

    African Journals Online (AJOL)

    Digital libraries are well known for sharing resources all over the world. Several ... The person with more information will guide a group or society and he ..... librarians should work out among themselves a co-operative means of tracking the.

  20. SYSTEM OF INCREASE OF LIBRARY WORKERS INFORMATIVE LITERACY

    Directory of Open Access Journals (Sweden)

    Svitlana M. Ivanova

    2011-02-01

    Full Text Available The article is devoted to the problems of modern information and communication technologies use in professional activity of library workers. The methods of increase of librarian informative literacy are offered. The special course aiming to prepare the skilled specialists who can decide innovative tasks on introduction, management and work with electronic resources is described. The tasks which will help listeners to enter in a theory and practice of electronic resources use in a modern library are decided in the course, such as: to form skills for working in electronic libraries; to teach the methods of modernization of informative librarian services on the base of technologies and resources of electronic libraries, freely Eprints software use.

  1. ORIGINAL ARTICLE Effect of Jimma University Libraries System ...

    African Journals Online (AJOL)

    WJH

    that impact negatively on quality education in Jimma University. Thus, the ... stakeholders/customers centered way. ... the misuse of the budget allocated for ..... Table 3: Relationship of JULS use and the purpose for the library materials use.

  2. Dynamic combinatorial libraries : new opportunities in systems chemistry

    NARCIS (Netherlands)

    Hunt, Rosemary A. R.; Otto, Sijbren; Hunt, Rosemary A.R.

    2011-01-01

    Combinatorial chemistry is a tool for selecting molecules with special properties. Dynamic combinatorial chemistry started off aiming to be just that. However, unlike ordinary combinatorial chemistry, the interconnectedness of dynamic libraries gives them an extra dimension. An understanding of

  3. NILDE, Network Inter Library Document Exchange: An Italian Document Delivery System

    Science.gov (United States)

    Brunetti, F.; Gasperini, A.; Mangiaracina, S.

    2007-10-01

    This poster presents NILDE, a document delivery system supporting the exchange of documents via the internet. The system has been set up by the Central Library of the National Research Council of Bologna (Italy) in order to make use of new internet technology, to promote cooperation between Italian university libraries and research libraries, and to achieve quick response times in satisfying DD requests. The Arcetri Astrophysical Observatory Library was the first astronomical library to join the NILDE project from its earliest days in 2002. Many were the reasons for this choice: automation of the DD processes, security and reliability of the network, creation of usage statistics and reports, reduction of DD System management costs and so on. This work describes the benefits of NILDE and discusses the role of an organized document delivery system as an important tool to cope with the difficult constraints of the publishing market.

  4. iTesla Power Systems Library (iPSL): A Modelica library for phasor time-domain simulations

    Science.gov (United States)

    Vanfretti, L.; Rabuzin, T.; Baudette, M.; Murad, M.

    The iTesla Power Systems Library (iPSL) is a Modelica package providing a set of power system components for phasor time-domain modeling and simulation. The Modelica language provides a systematic approach to develop models using a formal mathematical description, that uniquely specifies the physical behavior of a component or the entire system. Furthermore, the standardized specification of the Modelica language (Modelica Association [1]) enables unambiguous model exchange by allowing any Modelica-compliant tool to utilize the models for simulation and their analyses without the need of a specific model transformation tool. As the Modelica language is being developed with open specifications, any tool that implements these requirements can be utilized. This gives users the freedom of choosing an Integrated Development Environment (IDE) of their choice. Furthermore, any integration solver can be implemented within a Modelica tool to simulate Modelica models. Additionally, Modelica is an object-oriented language, enabling code factorization and model re-use to improve the readability of a library by structuring it with object-oriented hierarchy. The developed library is released under an open source license to enable a wider distribution and let the user customize it to their specific needs. This paper describes the iPSL and provides illustrative application examples.

  5. Construction of a 7-fold BAC library and cytogenetic mapping of 10 genes in the giant panda (Ailuropoda melanoleuca

    Directory of Open Access Journals (Sweden)

    Zhang Ying

    2006-11-01

    Full Text Available Abstract Background The giant panda, one of the most primitive carnivores, is an endangered animal. Although it has been the subject of many interesting studies during recent years, little is known about its genome. In order to promote research on this genome, a bacterial artificial chromosome (BAC library of the giant panda was constructed in this study. Results This BAC library contains 198,844 clones with an average insert size of 108 kb, which represents approximately seven equivalents of the giant panda haploid genome. Screening the library with 15 genes and 8 microsatellite markers demonstrates that it is representative and has good genome coverage. Furthermore, ten BAC clones harbouring AGXT, GHR, FSHR, IRBP, SOX14, TTR, BDNF, NT-4, LH and ZFX1 were mapped to 8 pairs of giant panda chromosomes by fluorescence in situ hybridization (FISH. Conclusion This is the first large-insert genomic DNA library for the giant panda, and will contribute to understanding this endangered species in the areas of genome sequencing, physical mapping, gene cloning and comparative genomic studies. We also identified the physical locations of ten genes on their relative chromosomes by FISH, providing a preliminary framework for further development of a high resolution cytogenetic map of the giant panda.

  6. OpenIPSL: Open-Instance Power System Library - Update 1.5 to "iTesla Power Systems Library (iPSL): A Modelica library for phasor time-domain simulations"

    Science.gov (United States)

    Baudette, Maxime; Castro, Marcelo; Rabuzin, Tin; Lavenius, Jan; Bogodorova, Tetiana; Vanfretti, Luigi

    2018-01-01

    This paper presents the latest improvements implemented in the Open-Instance Power System Library (OpenIPSL). The OpenIPSL is a fork from the original iTesla Power Systems Library (iPSL) by some of the original developers of the iPSL. This fork's motivation comes from the will of the authors to further develop the library with additional features tailored to research and teaching purposes. The enhancements include improvements to existing models, the addition of a new package of three phase models, and the implementation of automated tests through continuous integration.

  7. LibraryH3lp: A New Flexible Chat Reference System

    Directory of Open Access Journals (Sweden)

    Pam Sessoms

    2008-09-01

    Full Text Available LibraryH3lp is an integrated IM and web chat system designed specifically for Virtual Reference services in libraries. The software was designed for, and is currently used by, a night-time chat reference collaboraton between several large academic libraries. LibraryH3lp is designed for the workflow of chat reference, supporting multiple simultaneous operators and routing to queues of operators in a particular service area. It also supports web page embeddable chat 'widgets', as well as simultaneous gateways to multiple IM protocols. This article discusses the motivation for the development of the software, and provides an overview of LibraryH3lp's features and technical architecture. Parts of LibraryH3lp are available as open source. The complete application is available as a low-cost hosted service, and will eventually be available to be licensed for local hosting.

  8. Penerapan INLISlite (Integrated Library System di Kantor Perpustakaan dan Arsip Daerah Kabupaten Pangkep, Sulawesi Selatan

    Directory of Open Access Journals (Sweden)

    Abdul Hamid

    2015-12-01

    Full Text Available Information and communication technology (ICT has been undoubtly applied in all fields of life. It provides variety of services in which it is dependent on user needs. The Kantor Perpustakaan dan Arsip Daerah (the District Library and Archieve of Pangkep has been implementing ICT to enhance its services to the citizens of Pangkep. INSLISLite was choosen as its automted library system. The current study investigates how INLIS lite has been implemented in the library, what were the challanges the library faced, and the floating library services of KPAD. The data were collected throughout the representative informans. The study found that INLIS lite needed to be more optimized, the challenges were human resources and budgetary, and the floating library has played important roles to educate the Pangkep island citizens.

  9. Development of a 1200 fine group nuclear data library for advanced nuclear systems

    Institute of Scientific and Technical Information of China (English)

    Jun Zou; Lei-Ming Shang; Fang Wang; Li-Juan Hao

    2017-01-01

    Accurate and reliable nuclear data libraries are essential for calculation and design of advanced nuclear systems.A 1200 fine group nuclear data library Hybrid Evaluated Nuclear Data Library/Fine Group (HENDL/FG) with neutrons of up to 150 MeV has been developed to improve the accuracy of neutronics calculations and analysis.Corrections of Doppler,resonance self-shielding,and thermal upscatter effects were done for HENDL/FG.Shielding and critical safety benchmarks were performed to test the accuracy and reliability of the library.The discrepancy between calculated and measured nuclear parameters fell into a reasonable range.

  10. Investigating User Search Tactic Patterns and System Support in Using Digital Libraries

    Science.gov (United States)

    Joo, Soohyung

    2013-01-01

    This study aims to investigate users' search tactic application and system support in using digital libraries. A user study was conducted with sixty digital library users. The study was designed to answer three research questions: 1) How do users engage in a search process by applying different types of search tactics while conducting different…

  11. Self-Service Charge Systems: Current Technological Applications and Their Implications for the Future Library.

    Science.gov (United States)

    Mardikian, Jackie

    1995-01-01

    Discusses a possible shift to self-service check-out systems for academic library patrons. Provides an annotated bibliography of 51 items that discuss the impact of self-service technology on the quality of service and customer satisfaction in libraries, service sectors, banking, and the vending industry. (LRW)

  12. Automation Challenges of the 80's: What to Do until Your Integrated Library System Arrives.

    Science.gov (United States)

    Allan, Ferne C.; Shields, Joyce M.

    1986-01-01

    A medium-sized aerospace library has developed interim solutions to automation needs by using software and equipment that were available in-house in preparation for an expected integrated library system. Automated processes include authors' file of items authored by employees, journal routing (including routing slips), statistics, journal…

  13. Systems Analysis, Machineable Circulation Data and Library Users and Non-Users.

    Science.gov (United States)

    Lubans, John, Jr.

    A study to be made with computer-based circulation data of the non-use and use of a large academic library is discussed. A search of the literature reveals that computer-based circulation systems can be, but have not been, utilized to provide data bases for systematic analyses of library users and resources. The data gathered in the circulation…

  14. Library of neutron reaction cross-sections in the ABBN-93 constant system

    International Nuclear Information System (INIS)

    Zabrodskaya, S.V.; Korchagina, Zh.A.; Koshcheev, V.N.; Nikolaev, M.N.; Tsibulya, A.M.

    2001-01-01

    The library of neutron reaction group cross-sections in the ABBN-93 constant set is described. The format used for data representation, the content and purpose of the sub-libraries and their practical application in the SCALE criticality safety estimation system are discussed. (author)

  15. Linking Library Automation Systems in the Internet: Functional Requirements, Planning, and Policy Issues.

    Science.gov (United States)

    Lynch, Clifford A.

    1989-01-01

    This guide to functions to consider in selecting an academic library automation system to operate in a networked environment covers (1) the current academic networking environment; (2) library automation hardware and software platforms; (3) user interface requirements for public access; and (4) security and authentication. (10 references) (MES)

  16. Using geographic information systems to identify prospective marketing areas for a special library.

    Science.gov (United States)

    McConnaughy, Rozalynd P; Wilson, Steven P

    2006-05-04

    The Center for Disability Resources (CDR) Library is the largest collection of its kind in the Southeastern United States, consisting of over 5,200 books, videos/DVDs, brochures, and audiotapes covering a variety of disability-related topics, from autism to transition resources. The purpose of the library is to support the information needs of families, faculty, students, staff, and other professionals in South Carolina working with individuals with disabilities. The CDR Library is funded on a yearly basis; therefore, maintaining high usage is crucial. A variety of promotional efforts have been used to attract new patrons to the library. Anyone in South Carolina can check out materials from the library, and most of the patrons use the library remotely by requesting materials, which are then mailed to them. The goal of this project was to identify areas of low geographic usage as a means of identifying locations for future library marketing efforts. Nearly four years worth of library statistics were compiled in a spreadsheet that provided information per county on the number of checkouts, the number of renewals, and the population. Five maps were created using ArcView GIS software to create visual representations of patron checkout and renewal behavior per county. Out of the 46 counties in South Carolina, eight counties never checked out materials from the library. As expected urban areas and counties near the library's physical location have high usage totals. The visual representation of the data made identification of low usage regions easier than using a standalone database with no visual-spatial component. The low usage counties will be the focus of future Center for Disability Resources Library marketing efforts. Due to the impressive visual-spatial representations created with Geographic Information Systems, which more efficiently communicate information than stand-alone database information can, librarians may benefit from the software's use as a

  17. Development of library preparation method able to correct gene expression levels in rice anther and isolate a trace expression gene mediated in cold-resistance

    International Nuclear Information System (INIS)

    Yamaguchi, Tomoya; Koike, Setsuo

    2000-01-01

    When cDNA library is prepared by a previously developed method, genes of which expression level is high are apt to be cloned at a high frequency, whereas genes of which expression level are low, are difficult to be cloned. A low-expression gene has been cloned at very low frequency. Therefore, the gene encoding the key enzyme that is involved in growth disturbance of rice pollen has not been identified. In this study, development of a library preparing method able to correct the expression level was attempted using highly sensitive detection method with radioisotope and some genes related to cold-resistance of rice were isolated. Double strand DNAs were synthesized using mRNA extract from rice anthers and annealed following heat-denaturation. It has been known that single strand DNA molecules abundantly existing in DNA solution can easily aggregate to form double strand DNA, but single stranded DNA molecules poor in the solution are apt to still remain as single strand after annealing. Thus, the amount of single strand DNA would be balanced in the solution between abundant DNA and poor DNA species. The authors succeeded to prepare a gene library including low and high expression genes at similar proportions. Moreover, spin trap method that allows RI labeling of DNA bound to latex particle, was developed to detect with high sensitivity, especially for genes that are expressed at low level. The present method could be used for recovery, detection and quantitative analysis of radiolabeled single strand DNA. Thus, it was demonstrated that the stage from tetrad sperm to small sperm might be easily affected by cold stress. The present results suggest that the expressions of β-1 and β-3 glucanase, which are involved in the release of small sperms following meiosis in the pollen formation, might be easily affected by cold stress. (M.N.)

  18. Development of library preparation method able to correct gene expression levels in rice anther and isolate a trace expression gene mediated in cold-resistance

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, Tomoya; Koike, Setsuo [Tohoku National Agricultural Experiment Station, Morioka (Japan)

    2000-02-01

    When cDNA library is prepared by a previously developed method, genes of which expression level is high are apt to be cloned at a high frequency, whereas genes of which expression level are low, are difficult to be cloned. A low-expression gene has been cloned at very low frequency. Therefore, the gene encoding the key enzyme that is involved in growth disturbance of rice pollen has not been identified. In this study, development of a library preparing method able to correct the expression level was attempted using highly sensitive detection method with radioisotope and some genes related to cold-resistance of rice were isolated. Double strand DNAs were synthesized using mRNA extract from rice anthers and annealed following heat-denaturation. It has been known that single strand DNA molecules abundantly existing in DNA solution can easily aggregate to form double strand DNA, but single stranded DNA molecules poor in the solution are apt to still remain as single strand after annealing. Thus, the amount of single strand DNA would be balanced in the solution between abundant DNA and poor DNA species. The authors succeeded to prepare a gene library including low and high expression genes at similar proportions. Moreover, spin trap method that allows RI labeling of DNA bound to latex particle, was developed to detect with high sensitivity, especially for genes that are expressed at low level. The present method could be used for recovery, detection and quantitative analysis of radiolabeled single strand DNA. Thus, it was demonstrated that the stage from tetrad sperm to small sperm might be easily affected by cold stress. The present results suggest that the expressions of {beta}-1 and {beta}-3 glucanase, which are involved in the release of small sperms following meiosis in the pollen formation, might be easily affected by cold stress. (M.N.)

  19. Application of expert system in library and information science: a review

    International Nuclear Information System (INIS)

    Bhatti, A.R.

    1988-09-01

    This study seeks to provide an overview of the current state of development in the application of expert system in technology in the field of library and information science. The study starts briefly looking into the capabilities of Artificial Intelligence (AI) in general and then its relevance to LIS (Library Information System) work. A basic introduction to expert system technology and outgrowth of AI research is given. The major components of a generalized expert system are discussed in some detail, and the ways in which system development can be approached are looked at. The main area of expert system applications in library and information science are discussed, looking at cataloguing, classification, reference work and library management etc. (author)

  20. Archiving Spectral Libraries in the Planetary Data System

    Science.gov (United States)

    Slavney, S.; Guinness, E. A.; Scholes, D.; Zastrow, A.

    2017-12-01

    Spectral libraries are becoming popular candidates for archiving in PDS. With the increase in the number of individual investigators funded by programs such as NASA's PDART, the PDS Geosciences Node is receiving many requests for support from proposers wishing to archive various forms of laboratory spectra. To accommodate the need for a standardized approach to archiving spectra, the Geosciences Node has designed the PDS Spectral Library Data Dictionary, which contains PDS4 classes and attributes specifically for labeling spectral data, including a classification scheme for samples. The Reflectance Experiment Laboratory (RELAB) at Brown University, which has long been a provider of spectroscopy equipment and services to the science community, has provided expert input into the design of the dictionary. Together the Geosciences Node and RELAB are preparing the whole of the RELAB Spectral Library, consisting of many thousands of spectra collected over the years, to be archived in PDS. An online interface for searching, displaying, and downloading selected spectra is planned, using the Spectral Library metadata recorded in the PDS labels. The data dictionary and online interface will be extended to include spectral libraries submitted by other data providers. The Spectral Library Data Dictionary is now available from PDS at https://pds.nasa.gov/pds4/schema/released/. It can be used in PDS4 labels for reflectance spectra as well as for Raman, XRF, XRD, LIBS, and other types of spectra. Ancillary data such as images, chemistry, and abundance data are also supported. To help generate PDS4-compliant labels for spectra, the Geosciences Node provides a label generation program called MakeLabels (http://pds-geosciences.wustl.edu/tools/makelabels.html) which creates labels from a template, and which can be used for any kind of PDS4 label. For information, contact the Geosciences Node at geosci@wunder.wustl.edu.

  1. Effectiveness of caching in a distributed digital library system

    DEFF Research Database (Denmark)

    Hollmann, J.; Ardø, Anders; Stenstrom, P.

    2007-01-01

    as manifested by gateways that implement the interfaces to the many fulltext archives. A central research question in this approach is: What is the nature of locality in the user access stream to such a digital library? Based on access logs that drive the simulations, it is shown that client-side caching can......Today independent publishers are offering digital libraries with fulltext archives. In an attempt to provide a single user-interface to a large set of archives, the studied Article-Database-Service offers a consolidated interface to a geographically distributed set of archives. While this approach...

  2. Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library.

    Science.gov (United States)

    Tao, Bo; Shao, Bai-Hui; Qiao, Yu-Xin; Wang, Xiao-Qin; Chang, Shu-Jun; Qiu, Li-Juan

    2017-08-01

    Glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. If glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. While several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is interest in identifying additional genes for glyphosate tolerance. This research constructed a high-quality cDNA library form the glyphosate-resistant fungus Aspergillus oryzae RIB40 to identify genes that may confer resistance to glyphosate. Using a medium containing glyphosate (120mM), we screened several clones from the library. Based on a nucleotide sequence analysis, we identified a gene of unknown function (GenBank accession number: XM_001826835.2) that encoded a hypothetical 344-amino acid protein. The gene was named MFS40. Its ORF was amplified to construct an expression vector, pGEX-4T-1-MFS40, to express the protein in Escherichia coli BL21. The gene conferred glyphosate tolerance to E. coli ER2799 cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Graphical User Interfaces and Library Systems: End-User Reactions.

    Science.gov (United States)

    Zorn, Margaret; Marshall, Lucy

    1995-01-01

    Describes a study by Parke-Davis Pharmaceutical Research Library to determine user satisfaction with the graphical user interface-based (GUI) Dynix Marquis compared with the text-based Dynix Classic Online Public Access Catalog (OPAC). Results show that the GUI-based OPAC was preferred by endusers over the text-based OPAC. (eight references) (DGM)

  4. An Integrated System for Managing the Andalusian Parliament's Digital Library

    Science.gov (United States)

    de Campos, Luis M.; Fernandez-Luna, Juan M.; Huete, Juan F.; Martin-Dancausa, Carlos J.; Tagua-Jimenez, Antonio; Tur-Vigil, Carmen

    2009-01-01

    Purpose: The purpose of this paper is to present an overview of the reorganisation of the Andalusian Parliament's digital library to improve the electronic representation and access of its official corpus by taking advantage of a document's internal organisation. Video recordings of the parliamentary sessions have also been integrated with their…

  5. Publishing WWII aerial photographs in geographical and library information systems

    NARCIS (Netherlands)

    Verhelst, E.C.H.; Missel, L.; Vanmeulebrouk, B.; Rip, F.I.

    2012-01-01

    The Library of the Dutch Wageningen University and Research centre houses a collection of aerial photographs taken by the Allied Air Forces. The collection is part of a project that aims to publish these images in a user friendly way so that they are accessible to a wide audience. This paper

  6. A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus

    Directory of Open Access Journals (Sweden)

    Alamar Santiago

    2009-09-01

    Full Text Available Abstract Background Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences has the power to improve the quality of genome annotation. Results We developed an integrated method to generate a new normalized EST collection enriched in full-length and rare transcripts of different citrus species from multiple tissues and developmental stages. We constructed a total of 15 cDNA libraries, from which we isolated 10,898 high-quality ESTs representing 6142 different genes. Percentages of redundancy and proportion of full-length clones range from 8 to 33, and 67 to 85, respectively, indicating good efficiency of the approach employed. The new EST collection adds 2113 new citrus ESTs, representing 1831 unigenes, to the collection of citrus genes available in the public databases. To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta. Herein, we describe the technical methods used in the library construction, sequence analysis of clones and the overexpression of CitrSEP, a citrus homolog to the Arabidopsis SEP3 gene, in Arabidopsis as an example of a practical application of the engineered Gateway vector for functional analysis. Conclusion The new

  7. A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus

    Science.gov (United States)

    Marques, M Carmen; Alonso-Cantabrana, Hugo; Forment, Javier; Arribas, Raquel; Alamar, Santiago; Conejero, Vicente; Perez-Amador, Miguel A

    2009-01-01

    Background Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences has the power to improve the quality of genome annotation. Results We developed an integrated method to generate a new normalized EST collection enriched in full-length and rare transcripts of different citrus species from multiple tissues and developmental stages. We constructed a total of 15 cDNA libraries, from which we isolated 10,898 high-quality ESTs representing 6142 different genes. Percentages of redundancy and proportion of full-length clones range from 8 to 33, and 67 to 85, respectively, indicating good efficiency of the approach employed. The new EST collection adds 2113 new citrus ESTs, representing 1831 unigenes, to the collection of citrus genes available in the public databases. To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta. Herein, we describe the technical methods used in the library construction, sequence analysis of clones and the overexpression of CitrSEP, a citrus homolog to the Arabidopsis SEP3 gene, in Arabidopsis as an example of a practical application of the engineered Gateway vector for functional analysis. Conclusion The new EST collection denotes an

  8. USE OF LIBRARIES IN OPEN AND DISTANCE LEARNING SYSTEM: Barriers to the Use of AIOU Libraries by Tutors and Students

    Directory of Open Access Journals (Sweden)

    Abdul Jabbar BHATTI,

    2012-04-01

    Full Text Available This study explores; the library needs of students and tutors of Allama Iqbal Open University (AIOU, utilization level of the library facilities and resources, the problems in the use of library, and suggestions for improvement of library facilities for students and tutors. Data collected from 4080 students and 526 tutors belonging to 15 different regional offices showed that students and tutors needed library for various educational purposes, the regional libraries were not being used much, and both tutors and students were facing various problems such as unsuitable library timing, long distance between library and their residence, non availability of latest journals, non availability of required material, lack of temperature control in the library, insufficient study area, lack of latest books, and inadequate staff. For improving library facility at regional level, the students and tutors suggested to; provide more books and journals, expand library timings, arrange library facility at workshop venues, make arrangements to advertise the resources and services at the library to the students, provide computers and internet service, provide trained staff, and arrange partnership with other academic libraries.

  9. Nuclear models, experiments and data libraries needed for numerical simulation of accelerator-driven system

    International Nuclear Information System (INIS)

    Bauge, E.; Bersillon, O.

    2000-01-01

    This paper presents the transparencies of the speech concerning the nuclear models, experiments and data libraries needed for numerical simulation of Accelerator-Driven Systems. The first part concerning the nuclear models defines the spallation process, the corresponding models (intra-nuclear cascade, statistical model, Fermi breakup, fission, transport, decay and macroscopic aspects) and the code systems. The second part devoted to the experiments presents the angular measurements, the integral measurements, the residual nuclei and the energy deposition. In the last part, dealing with the data libraries, the author details the fundamental quantities as the reaction cross-section, the low energy transport databases and the decay libraries. (A.L.B.)

  10. Neutron cross section library production code system for continuous energy Monte Carlo code MVP. LICEM

    International Nuclear Information System (INIS)

    Mori, Takamasa; Nakagawa, Masayuki; Kaneko, Kunio.

    1996-05-01

    A code system has been developed to produce neutron cross section libraries for the MVP continuous energy Monte Carlo code from an evaluated nuclear data library in the ENDF format. The code system consists of 9 computer codes, and can process nuclear data in the latest ENDF-6 format. By using the present system, MVP neutron cross section libraries for important nuclides in reactor core analyses, shielding and fusion neutronics calculations have been prepared from JENDL-3.1, JENDL-3.2, JENDL-FUSION file and ENDF/B-VI data bases. This report describes the format of MVP neutron cross section library, the details of each code in the code system and how to use them. (author)

  11. Neutron cross section library production code system for continuous energy Monte Carlo code MVP. LICEM

    Energy Technology Data Exchange (ETDEWEB)

    Mori, Takamasa; Nakagawa, Masayuki [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Kaneko, Kunio

    1996-05-01

    A code system has been developed to produce neutron cross section libraries for the MVP continuous energy Monte Carlo code from an evaluated nuclear data library in the ENDF format. The code system consists of 9 computer codes, and can process nuclear data in the latest ENDF-6 format. By using the present system, MVP neutron cross section libraries for important nuclides in reactor core analyses, shielding and fusion neutronics calculations have been prepared from JENDL-3.1, JENDL-3.2, JENDL-FUSION file and ENDF/B-VI data bases. This report describes the format of MVP neutron cross section library, the details of each code in the code system and how to use them. (author).

  12. Visual system of recovering and combination of information for ENDF (Evaluated Nuclear Data File) format libraries

    International Nuclear Information System (INIS)

    Ferreira, Claudia A.S. Velloso; Corcuera, Raquel A. Paviotti

    1997-01-01

    This report presents a data information retrieval and merger system for ENDF (Evaluated Nuclear Data File) format libraries, which can be run on personal computers under the Windows TM environment. The input is the name of an ENDF/B library, which can be chosen in a proper window. The system has a display function which allows the user to visualize the reaction data of a specific nuclide and to produce a printed copy of these data. The system allows the user to retrieve and/or combine evaluated data to create a single file of data in ENDF format, from a number of different files, each of which is in the ENDF format. The user can also create a mini-library from an ENDF/B library. This interactive and easy-to-handle system is a useful tool for Nuclear Data Centers and it is also of interest to nuclear and reactor physics researchers. (author)

  13. 16S rRNA Gene Sequence Analysis of Drinking Water Using RNA and DNA Extracts as Targets for Clone Library Development

    Science.gov (United States)

    The bacterial composition of chlorinated drinking water was analyzed using 16S rRNA gene clone libraries derived from DNA extracts of 12 samples and compared to clone libraries previously generated using RNA extracts from the same samples. Phylogenetic analysis of 761 DNA-based ...

  14. MyWelch: building an information portal system in a medical library environment.

    Science.gov (United States)

    Zhang, Dongming; Zambrowicz, Caroline; Zhou, Hong; Roderer, Nancy

    2003-01-01

    MyWelch is a medical library portal system that users can use to create customized web sites that reflect their research needs and personal interests. In the MyWelch environment, faculty and students are empowered to take a greater role in identifying their needs and determining their requirements in the electronic environment. The portal system also facilitates interaction among library users and staff.

  15. Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis.

    Science.gov (United States)

    Zakaria, M N; Takeshita, T; Shibata, Y; Maeda, H; Wada, N; Akamine, A; Yamashita, Y

    2015-08-01

    To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions associated with root filled teeth. DNA was directly extracted from each sample and the microbial composition was comprehensively analysed using clone library analysis of the 16S rRNA gene. Enterococcus faecalis, Candida albicans and specific fimA genotypes of Porphyromonas gingivalis were confirmed using polymerase chain reaction (PCR) analysis with specific primers. Bacteria were detected in all samples, and the dominant findings were P. gingivalis (19.9%), Fusobacterium nucleatum (11.2%) and Propionibacterium acnes (9%). Bacterial diversity was greater in symptomatic lesions than in asymptomatic ones. In addition, the following bacteria or bacterial combinations were characteristic to symptomatic lesions: Prevotella spp., Treponema spp., Peptostreptococcaceae sp. HOT-113, Olsenella uli, Slackia exigua, Selemonas infelix, P. gingivalis with type IV fimA, and a combination of P. gingivalis, F. nucleatum, and Peptostreptococcaceae sp. HOT-113 and predominance of Streptococcus spp. On the other hand, neither Enterococcus faecalis nor C. albicans were detected in any of the samples. Whilst a diverse bacterial species were observed in the persistent apical lesions, some characteristic patterns of bacterial community were found in the symptomatic lesions. The diverse variation of community indicates that bacterial combinations as a community may cause persistent inflammation in periapical tissues rather than specific bacterial species. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

  16. STATLIB, Interactive Statistics Program Library of Tutorial System

    International Nuclear Information System (INIS)

    Anderson, H.E.

    1986-01-01

    1 - Description of program or function: STATLIB is a conversational statistical program library developed in conjunction with a Sandia National Laboratories applied statistics course intended for practicing engineers and scientists. STATLIB is a group of 15 interactive, argument-free, statistical routines. Included are analysis of sensitivity tests; sample statistics for the normal, exponential, hypergeometric, Weibull, and extreme value distributions; three models of multiple regression analysis; x-y data plots; exact probabilities for RxC tables; n sets of m permuted integers in the range 1 to m; simple linear regression and correlation; K different random integers in the range m to n; and Fisher's exact test of independence for a 2 by 2 contingency table. Forty-five other subroutines in the library support the basic 15

  17. Methylation-sensitive linking libraries enhance gene-enriched sequencing of complex genomes and map DNA methylation domains

    Directory of Open Access Journals (Sweden)

    Bharti Arvind K

    2008-12-01

    Full Text Available Abstract Background Many plant genomes are resistant to whole-genome assembly due to an abundance of repetitive sequence, leading to the development of gene-rich sequencing techniques. Two such techniques are hypomethylated partial restriction (HMPR and methylation spanning linker libraries (MSLL. These libraries differ from other gene-rich datasets in having larger insert sizes, and the MSLL clones are designed to provide reads localized to "epigenetic boundaries" where methylation begins or ends. Results A large-scale study in maize generated 40,299 HMPR sequences and 80,723 MSLL sequences, including MSLL clones exceeding 100 kb. The paired end reads of MSLL and HMPR clones were shown to be effective in linking existing gene-rich sequences into scaffolds. In addition, it was shown that the MSLL clones can be used for anchoring these scaffolds to a BAC-based physical map. The MSLL end reads effectively identified epigenetic boundaries, as indicated by their preferential alignment to regions upstream and downstream from annotated genes. The ability to precisely map long stretches of fully methylated DNA sequence is a unique outcome of MSLL analysis, and was also shown to provide evidence for errors in gene identification. MSLL clones were observed to be significantly more repeat-rich in their interiors than in their end reads, confirming the correlation between methylation and retroelement content. Both MSLL and HMPR reads were found to be substantially gene-enriched, with the SalI MSLL libraries being the most highly enriched (31% align to an EST contig, while the HMPR clones exhibited exceptional depletion of repetitive DNA (to ~11%. These two techniques were compared with other gene-enrichment methods, and shown to be complementary. Conclusion MSLL technology provides an unparalleled approach for mapping the epigenetic status of repetitive blocks and for identifying sequences mis-identified as genes. Although the types and natures of

  18. Study of college library appealing information system: A case of Longyan University

    Science.gov (United States)

    Liao, Jin-Hui

    2014-10-01

    The complaints from the readers at university libraries mainly focus on the aspects of service attitude, quality of service, reading environment, the management system, etc. Librarians should realize that reader complaints can actually promote the role of the library service and communicate with readers who complain in a friendly manner. In addition, the Longyan University library should establish an internal management system, improve library hardware facilities, improve the quality of librarians and optimize the knowledge structure of librarians, so as to improve the quality of the service for readers and reduce complaints. Based on this point, we have designed an appealing information system in cryptography machine basis, to provide readers online, remote and anonymous complaint functions.

  19. VALGALI: VERIFICATION AND VALIDATION TOOL FOR THE LIBRARIES PRODUCED BY THE GALILEE SYSTEM

    International Nuclear Information System (INIS)

    Mengelle, S.

    2011-01-01

    Full text: In this paper we present VALGALI the verification and validation tool for the libraries produced by the nuclear data processing system GALILEE. The aim of this system is to provide libraries with consistent physical data for various application codes (the deterministic transport code APOLLO2, the Monte Carlo transport code TRRIPOLI-4, the depletion code DARWIN, ...). For each library, are the data stored at the good place with the good format and so one. Are the libraries used by the various codes consistent. What is the physical quality of the cross sections and data present in the libraries. These three types of tests correspond to the classic stages of VandV. The great strength of VALGALI is to be generic and not dedicated to one application code Consequently, it is based on a common physical validation database which coverage is regularly increased. For all these test cases, the input data are declined for each relevant application code Moreover it can exist specific test case for each application code: At the present, VALGALI an check and validate the libraries of APOLLO2 and TRIPOLI4, but in the near future VALGALI wil also treat the libraries of DARWIN.

  20. Development of Calcomp compatible interface library 'piflib' on X Window System

    International Nuclear Information System (INIS)

    Tanabe, Hidenobu; Yokokawa, Mitsuo; Onuma, Yoshio.

    1993-05-01

    Graphics processing at JAERI mainly has been executed on mainframe computers with Calcomp compatible graphics libraries. With spread of engineering workstations (EWS), it is important that those enormous graphics softwares be able to be carried out on EWS. The Calcomp compatible interface library 'piflib' has been developed on the X Window System, which is the most popular window environments on EWS. In this report, specifications of the library 'piflib' and its usages are presented. The cooperative processing with mainframe computers is also described. (author)

  1. Porting of the DBCSR library for Sparse Matrix-Matrix Multiplications to Intel Xeon Phi systems

    OpenAIRE

    Bethune, Iain; Gloess, Andeas; Hutter, Juerg; Lazzaro, Alfio; Pabst, Hans; Reid, Fiona

    2017-01-01

    Multiplication of two sparse matrices is a key operation in the simulation of the electronic structure of systems containing thousands of atoms and electrons. The highly optimized sparse linear algebra library DBCSR (Distributed Block Compressed Sparse Row) has been specifically designed to efficiently perform such sparse matrix-matrix multiplications. This library is the basic building block for linear scaling electronic structure theory and low scaling correlated methods in CP2K. It is para...

  2. Research on the Impact of a Computerized Circulation System on the Performance of a Large College Library. Final Report.

    Science.gov (United States)

    Frohmberg, Katherine A.; Moffett, William A.

    In order to study the effects of introducing an automated circulation system at Oberlin College, Ohio, data were collected from September 1978 until June 1982 on book availability, usage of library facilities, attitudes of library users toward the library, and the efficiency of circulation activities. Data collection methods included circulation…

  3. Cloning of low dose radiation induced gene RIG1 by RACE based on non-cloned cDNA library

    International Nuclear Information System (INIS)

    Luo Ying; Sui Jianli; Tie Yi; Zhang Yuanping; Zhou Pingkun; Sun Zhixian

    2001-01-01

    Objective: To obtain full-length cDNA of radiation induced new gene RIG1 based on its EST fragment. Methods: Based on non-cloned cDNA library, enhanced nested RACE PCR and biotin-avidin labelled probe for magnetic bead purification was used to obtain full-length cDNA of RIG1. Results: About 1 kb of 3' end of RIG1 gene was successfully cloned by this set of methods and cloning of RIG1 5' end is proceeding well. Conclusion: The result is consistent with the design of experiment. This set of protocol is useful for cloning of full-length gene based on EST fragment

  4. Geographic Information Systems: Tools for Displaying In-Library Use Data

    Directory of Open Access Journals (Sweden)

    Lauren H. Mandel

    2010-03-01

    Full Text Available In-library use data is crucial for modern libraries to understand the full spectrum of patron use, including patron self-service activities, circulation, and reference statistics. Rather than using tables and charts to display use data, a geographic information system (GIS facilitates a more visually appealing graphical display of the data in the form of a map. GISs have been used by library and information science (LIS researchers and practitioners to create maps that display analyses of service area populations and demographics, facilities space management issues, spatial distribution of in-library use of materials, planned branch consolidations, and so on. The “seating sweeps” method allows researchers and librarians to collect in-library use data regarding where patrons are locating themselves within the library and what they are doing at those locations, such as sitting and reading, studying in a group, or socializing. This paper proposes a GIS as a tool to visually display in-library use data collected via “seating sweeps” of a library. By using a GIS to store, manage, and display the data, researchers and librarians can create visually appealing maps that show areas of heavy use and evidence of the use and value of the library for a community. Example maps are included to facilitate the reader’s understanding of the possibilities afforded by using GISs in LIS research.

  5. Development library of finite elements for computer-aided design system of reed sensors

    Science.gov (United States)

    Kozlov, A. S.; Shmakov, N. A.; Tkalich, V. L.; Labkovskaia, R. I.; Kalinkina, M. E.; Pirozhnikova, O. I.

    2018-05-01

    The article is devoted to the development of a modern highly reliable element base of devices for security and fire alarm systems, in particular, to the improvement of the quality of contact cores (reed and membrane) of reed sensors. Modeling of elastic sensitive elements uses quadrangular elements of plates and shells, considered in the system of curvilinear orthogonal coordinates. The developed mathematical models and the formed finite element library are designed for systems of automated design of reed switch detectors to create competitive devices alarms. The finite element library is used for the automated system production of reed switch detectors both in series production and in the implementation of individual orders.

  6. Security of Heterogeneous Content in Cloud Based Library Information Systems Using an Ontology Based Approach

    Directory of Open Access Journals (Sweden)

    Mihai DOINEA

    2014-01-01

    Full Text Available As in any domain that involves the use of software, the library information systems take advantages of cloud computing. The paper highlights the main aspect of cloud based systems, describing some public solutions provided by the most important players on the market. Topics related to content security in cloud based services are tackled in order to emphasize the requirements that must be met by these types of systems. A cloud based implementation of an Information Library System is presented and some adjacent tools that are used together with it to provide digital content and metadata links are described. In a cloud based Information Library System security is approached by means of ontologies. Aspects such as content security in terms of digital rights are presented and a methodology for security optimization is proposed.

  7. A model library for simulation and benchmarking of integrated urban wastewater systems

    DEFF Research Database (Denmark)

    Saagi, R.; Flores Alsina, Xavier; Kroll, J. S.

    2017-01-01

    This paper presents a freely distributed, open-source toolbox to predict the behaviour of urban wastewater systems (UWS). The proposed library is used to develop a system-wide Benchmark Simulation Model (BSM-UWS) for evaluating (local/global) control strategies in urban wastewater systems (UWS...

  8. Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes.

    Science.gov (United States)

    Lee, Nam-Kyung; Bidlingmaier, Scott; Su, Yang; Liu, Bin

    2018-01-01

    Monoclonal antibodies and antibody-derived therapeutics have emerged as a rapidly growing class of biological drugs for the treatment of cancer, autoimmunity, infection, and neurological diseases. To support the development of human antibodies, various display techniques based on antibody gene repertoires have been constructed over the last two decades. In particular, scFv-antibody phage display has been extensively utilized to select lead antibodies against a variety of target antigens. To construct a scFv phage display that enables efficient antibody discovery, and optimization, it is desirable to develop a system that allows modular assembly of highly diverse variable heavy chain and light chain (Vκ and Vλ) repertoires. Here, we describe modular construction of large non-immune human antibody phage-display libraries built on variable gene cassettes from heavy chain and light chain repertoires (Vκ- and Vλ-light can be made into independent cassettes). We describe utility of such libraries in antibody discovery and optimization through chain shuffling.

  9. libmpdata++ 1.0: a library of parallel MPDATA solvers for systems of generalised transport equations

    Science.gov (United States)

    Jaruga, A.; Arabas, S.; Jarecka, D.; Pawlowska, H.; Smolarkiewicz, P. K.; Waruszewski, M.

    2015-04-01

    This paper accompanies the first release of libmpdata++, a C++ library implementing the multi-dimensional positive-definite advection transport algorithm (MPDATA) on regular structured grid. The library offers basic numerical solvers for systems of generalised transport equations. The solvers are forward-in-time, conservative and non-linearly stable. The libmpdata++ library covers the basic second-order-accurate formulation of MPDATA, its third-order variant, the infinite-gauge option for variable-sign fields and a flux-corrected transport extension to guarantee non-oscillatory solutions. The library is equipped with a non-symmetric variational elliptic solver for implicit evaluation of pressure gradient terms. All solvers offer parallelisation through domain decomposition using shared-memory parallelisation. The paper describes the library programming interface, and serves as a user guide. Supported options are illustrated with benchmarks discussed in the MPDATA literature. Benchmark descriptions include code snippets as well as quantitative representations of simulation results. Examples of applications include homogeneous transport in one, two and three dimensions in Cartesian and spherical domains; a shallow-water system compared with analytical solution (originally derived for a 2-D case); and a buoyant convection problem in an incompressible Boussinesq fluid with interfacial instability. All the examples are implemented out of the library tree. Regardless of the differences in the problem dimensionality, right-hand-side terms, boundary conditions and parallelisation approach, all the examples use the same unmodified library, which is a key goal of libmpdata++ design. The design, based on the principle of separation of concerns, prioritises the user and developer productivity. The libmpdata++ library is implemented in C++, making use of the Blitz++ multi-dimensional array containers, and is released as free/libre and open-source software.

  10. libmpdata++ 0.1: a library of parallel MPDATA solvers for systems of generalised transport equations

    Science.gov (United States)

    Jaruga, A.; Arabas, S.; Jarecka, D.; Pawlowska, H.; Smolarkiewicz, P. K.; Waruszewski, M.

    2014-11-01

    This paper accompanies first release of libmpdata++, a C++ library implementing the Multidimensional Positive-Definite Advection Transport Algorithm (MPDATA). The library offers basic numerical solvers for systems of generalised transport equations. The solvers are forward-in-time, conservative and non-linearly stable. The libmpdata++ library covers the basic second-order-accurate formulation of MPDATA, its third-order variant, the infinite-gauge option for variable-sign fields and a flux-corrected transport extension to guarantee non-oscillatory solutions. The library is equipped with a non-symmetric variational elliptic solver for implicit evaluation of pressure gradient terms. All solvers offer parallelisation through domain decomposition using shared-memory parallelisation. The paper describes the library programming interface, and serves as a user guide. Supported options are illustrated with benchmarks discussed in the MPDATA literature. Benchmark descriptions include code snippets as well as quantitative representations of simulation results. Examples of applications include: homogeneous transport in one, two and three dimensions in Cartesian and spherical domains; shallow-water system compared with analytical solution (originally derived for a 2-D case); and a buoyant convection problem in an incompressible Boussinesq fluid with interfacial instability. All the examples are implemented out of the library tree. Regardless of the differences in the problem dimensionality, right-hand-side terms, boundary conditions and parallelisation approach, all the examples use the same unmodified library, which is a key goal of libmpdata++ design. The design, based on the principle of separation of concerns, prioritises the user and developer productivity. The libmpdata++ library is implemented in C++, making use of the Blitz++ multi-dimensional array containers, and is released as free/libre and open-source software.

  11. An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies.

    Science.gov (United States)

    Frentiu, Francesca D; Adamski, Marcin; McGraw, Elizabeth A; Blows, Mark W; Chenoweth, Stephen F

    2009-01-21

    The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST) collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs), with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at http://www.chenowethlab.org/serrata/index.cgi. We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST collection will provide much needed genomic resources for

  12. Modelica Library for Building Heating, Ventilation and Air-Conditioning Systems

    Energy Technology Data Exchange (ETDEWEB)

    Wetter, Michael

    2009-06-17

    This paper presents a freely available Modelica library for building heating, ventilation and air conditioning systems. The library is based on the Modelica.Fluid library. It has been developed to support research and development of integrated building energy and control systems. The primary applications are controls design, energy analysis and model-based operation. The library contains dynamic and steady-state component models that are applicable for analyzing fast transients when designing control algorithms and for conducting annual simulations when assessing energy performance. For most models, dimensional analysis is used to compute the performance for operating points that differ from nominal conditions. This allows parameterizing models in the absence of detailed geometrical information which is often impractical to obtain during the conceptual design phase of building systems. In the first part of this paper, the library architecture and the main classes are described. In the second part, an example is presented in which we implemented a model of a hydronic heating system with thermostatic radiator valves and thermal energy storage.

  13. Debunking the Librarian "Gene": Designing Online Information Literacy Instruction for Incoming Library Science Students

    Science.gov (United States)

    Lamb, Annette

    2017-01-01

    Information workers are not born information fluent. Like other students, incoming library science students enter graduate programs with a broad range of information and technology skills. The aim of this study was to determine if systematically designed online tutorials would be effective in preparing university students with information literacy…

  14. Knowledgeability of Copyright Law among Librarians and Library Paraprofessionals Employed in Adult Services at a Large Public Library System.

    Science.gov (United States)

    Lavelle, Bridget M.

    Since public libraries contain copyrighted works in the form of print, electronic or audiovisual sources, librarians and library paraprofessionals need to possess sufficient knowledge of United States copyright law to meet the information needs of patrons successfully and legally. A literature review revealed that minimal works address this topic.…

  15. BIBLIO: A Computer System Designed to Support the Near-Library User Model of Information Retrieval.

    Science.gov (United States)

    Belew, Richard K.; Holland, Maurita Peterson

    1988-01-01

    Description of the development of the Information Exchange Facility, a prototype microcomputer-based personal bibliographic facility, covers software selection, user selection, overview of the system, and evaluation. The plan for an integrated system, BIBLIO, and the future role of libraries are discussed. (eight references) (MES)

  16. An Integrated Online Library System as a Node in a Local Area Network: The Mitre Experience.

    Science.gov (United States)

    Kidwell, Mary Coyle

    1987-01-01

    Discusses the Mitre Corporation's implementation of OCLC's LS/2000 integrated library system using a local area network (LAN). LAN issues--requirements, equipment, reliability, growth, security, and traffic--are covered in general and as they relate to Mitre. Installation of the LAN/system interface and benefits and drawbacks of using a LAN for…

  17. Why Mission-Critical Systems Are Critical to the Future of Academic Libraries

    Science.gov (United States)

    Oberlander, Cyril

    2012-01-01

    A mission-critical system is one that is so intertwined with the operation of an organization that the organization can scarcely function without it. Just as in corporations, mission-critical library systems offer the capability to unlock talent and time. They are essential to the transformation of higher education and the learning environment. A…

  18. An Evaluation of the Informedia Digital Video Library System at the Open University.

    Science.gov (United States)

    Kukulska-Hulme, Agnes; Van der Zwan, Robert; DiPaolo, Terry; Evers, Vanessa; Clarke, Sarah

    1999-01-01

    Reports on an Open University evaluation study of the Informedia Digital Video Library System developed at Carnegie Mellon University (CMU). Findings indicate that there is definite potential for using the system, provided that certain modifications can be made. Results also confirm findings of the Informedia team at CMU that the content of video…

  19. The Public Library on the Electronic Frontier: Starting a Community Online Information System.

    Science.gov (United States)

    Fox, Carol J.

    1995-01-01

    Details the Rockford (Illinois) Public Library's activities involved with developing a community online information system and the not-for-profit organization established to run the system. Includes mission statement; guiding principles, policy statements; standing committees and goals for first year of operation; funding; adding users and…

  20. Automated Circulation Systems in Libraries Serving the Blind and Physically Handicapped: A Reference Guide for Planning.

    Science.gov (United States)

    Wanger, Judith; And Others

    Designed to facilitate communications in future automation projects between library and data processing personnel, especially those projects involving the use of automated systems in the service of disabled patrons, this guide identifies and describes a master set of major circulation system requirements and design considerations, and illustrates…

  1. Implementation of an Integrated Information Management System at the National Library of Wales: A Case Study

    Science.gov (United States)

    Evans, Manon Foster; Thomas, Sian

    2007-01-01

    Purpose: This paper aims to describe the experiences of the National Library of Wales in implementing an integrated information management system. Design/methodology/approach: Discusses the stages involved in the procurement process, data migration and general system implementation. Findings: Emphasises the need for a well-prepared yet flexible…

  2. Apoptosis Gene Information System--AGIS.

    Science.gov (United States)

    Sakharkar, Kishore R; Clement, Marie V; Chow, Vincent T K; Pervaiz, Shazib

    2006-05-01

    Genes implicated in apoptosis have great relevance to biology, medicine and oncology. Here, we describe a unique resource, Apoptosis Gene Information System (AGIS) that provides data for over 2400 genes involved directly or indirectly, in apoptotic pathways of more than 350 different organisms. The organization of this information system is based on the principle of one-gene, one record. AGIS will be updated on a six monthly basis as new information becomes available. AGIS can be accessed at: http://www.cellfate.org/AGIS/.

  3. The Identification of Genes Important in Pseudomonas syringae pv. phaseolicola Plant Colonisation Using In Vitro Screening of Transposon Libraries.

    Directory of Open Access Journals (Sweden)

    Bharani Manoharan

    Full Text Available The bacterial plant pathogen Pseudomonas syringae pv. phaseolicola (Pph colonises the surface of common bean plants before moving into the interior of plant tissue, via wounds and stomata. In the intercellular spaces the pathogen proliferates in the apoplastic fluid and forms microcolonies (biofilms around plant cells. If the pathogen can suppress the plant's natural resistance response, it will cause halo blight disease. The process of resistance suppression is fairly well understood, but the mechanisms used by the pathogen in colonisation are less clear. We hypothesised that we could apply in vitro genetic screens to look for changes in motility, colony formation, and adhesion, which are proxies for infection, microcolony formation and cell adhesion. We made transposon (Tn mutant libraries of Pph strains 1448A and 1302A and found 106/1920 mutants exhibited alterations in colony morphology, motility and biofilm formation. Identification of the insertion point of the Tn identified within the genome highlighted, as expected, a number of altered motility mutants bearing mutations in genes encoding various parts of the flagellum. Genes involved in nutrient biosynthesis, membrane associated proteins, and a number of conserved hypothetical protein (CHP genes were also identified. A mutation of one CHP gene caused a positive increase in in planta bacterial growth. This rapid and inexpensive screening method allows the discovery of genes important for in vitro traits that can be correlated to roles in the plant interaction.

  4. Survey of Integration Cost-Adoption between Digital Library Systems in Iran

    Directory of Open Access Journals (Sweden)

    Mehdi Alipour-Hafezi

    2013-03-01

    Full Text Available The main goal of this article was identifying cost elements in syntactic integrating digital library systems in Iran. The levels of integration are content, technical, and organizational. It is obvious we could access some sub goals such as identifying current situation of information systems from the view points of data storage, needed standard outputs, and the current situation of interoperability in Iran, and suitable integration model in Iranian digital libraries. The analytical survey method was used in this research. Research population included 11 digital library systems that were used in Iranian digital libraries. In order to gather information, a researcher made questionnaire was used because of not existing standard collecting data tool. Findings demonstrated that we should search on three levels of interoperability: content, technical, and organizational level in order to identifying cost-adoption elements in syntactic interoperability. Also, findings showed that the elements of organizational level were too important level in cost-adoption elements. Also this research demonstrated that the high cost of adoption was related to libraries and their organizations.

  5. Design of books inventory with RFID antenna in library management system

    Directory of Open Access Journals (Sweden)

    Cheng Ching-Chien

    2017-01-01

    Full Text Available A 915 MHz printed radio-frequency-identification (RFID antennas with the characteristics of good gain and omnidirectional beam wave is constructed and evaluated in this study. The objective is to find out their best reading rates for providing effective wireless communications among RFID antenna during the library book inventory process. And an optimal library inventory system which is based on electromagnetic identification (EMID technology is proposed, which is constructed to find the optimal tag location for a book, test the tag readability for bookshelves, and connect a couple of multi-layer bookshelves with multiplexers and updat the tag reading status in the database of the computer terminal. The fabricated antenna reader and the proposed system are embedded into different locations of bookshelves and tested at the library of Cheng Shiu University in Taiwan. According to the experimental results, the designed prototype of the antenna reader has the characteristics of the directional radiation pattern, good gain, simple shape, low cost and is easy to be integrated into the bookshelf. And the designed library inventory system can authenticate the location of a book automatically. They can benefit administrating librarians with the capabilities of decreasing the library inventory processing time and reducing the possibility of the books being misplaced.

  6. Microaspiration of esophageal gland cells and cDNA library construction for identifying parasitism genes of plant-parasitic nematodes.

    Science.gov (United States)

    Hussey, Richard S; Huang, Guozhong; Allen, Rex

    2011-01-01

    Identifying parasitism genes encoding proteins secreted from a plant-parasitic nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding the molecular basis of nematode parasitism of plants. Parasitism genes have been cloned by directly microaspirating the cytoplasm from the esophageal gland cells of different parasitic stages of cyst or root-knot nematodes to provide mRNA to create a gland cell-specific cDNA library by long-distance reverse-transcriptase polymerase chain reaction. cDNA clones are sequenced and deduced protein sequences with a signal peptide for secretion are identified for high-throughput in situ hybridization to confirm gland-specific expression.

  7. Library system for a one dimensional tokamak transport code: (LIBJT60), 1

    International Nuclear Information System (INIS)

    Hirayama, Toshio

    1982-12-01

    A library system is developed to control and manage huge programs in terms of FORTRAN source. It is applied to widely used one dimensional tokamak transport codes (LIBJT60), which have been developed in the Division of Large Tokamak Development. The structure of data and program in the transport code turn out to be flexible enough to respond to various demands and this gigantic code frame work can be decomposed into groups of a compact code with a specific function. Some editing support tools for programming and debugging are also developed to save programming work. By applying this library system, users can obtain a code whose functions can be efficiently developed. (author)

  8. FY 2009 Public Libraries Survey

    Data.gov (United States)

    Institute of Museum and Library Services — Dig into FY 2009 data on public library systems (referred to as administrative entities in the Public Libraries Survey) and main libraries, branches, and bookmobiles...

  9. FY 2010 Public Libraries Survey

    Data.gov (United States)

    Institute of Museum and Library Services — Dig into FY 2010 data on public library systems (referred to as administrative entities in the Public Libraries Survey) and main libraries, branches, and bookmobiles...

  10. FY 2011 Public Libraries Survey

    Data.gov (United States)

    Institute of Museum and Library Services — Dig into FY 2011 data on public library systems (referred to as administrative entities in the Public Libraries Survey) and main libraries, branches, and bookmobiles...

  11. FY 2008 Public Libraries Survey

    Data.gov (United States)

    Institute of Museum and Library Services — Dig into FY 2008 data on public library systems (referred to as administrative entities in the Public Libraries Survey) and main libraries, branches, and bookmobiles...

  12. Differential distribution and abundance of diazotrophic bacterial communities across different soil niches using a gene-targeted clone library approach.

    Science.gov (United States)

    Yousuf, Basit; Kumar, Raghawendra; Mishra, Avinash; Jha, Bhavanath

    2014-11-01

    Diazotrophs are key players of the globally important biogeochemical nitrogen cycle, having a significant role in maintaining ecosystem sustainability. Saline soils are pristine and unexplored habitats representing intriguing ecosystems expected to harbour potential diazotrophs capable of adapting in extreme conditions, and these implicated organisms are largely obscure. Differential occurrence of diazotrophs was studied by the nifH gene-targeted clone library approach. Four nifH gene clone libraries were constructed from different soil niches, that is saline soils (low and high salinity; EC 3.8 and 7.1 ds m(-1) ), and agricultural and rhizosphere soil. Additionally, the abundance of diazotrophic community members was assessed using quantitative PCR. Results showed environment-dependent metabolic versatility and the presence of nitrogen-fixing bacteria affiliated with a range of taxa, encompassing members of the Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, Cyanobacteria and Firmicutes. The analyses unveiled the dominance of Alphaproteobacteria and Gammaproteobacteria (Pseudomonas, Halorhodospira, Ectothiorhodospira, Bradyrhizobium, Agrobacterium, Amorphomonas) as nitrogen fixers in coastal-saline soil ecosystems, and Alphaproteobacteria and Betaproteobacteria (Bradyrhizobium, Azohydromonas, Azospirillum, Ideonella) in agricultural/rhizosphere ecosystems. The results revealed a repertoire of novel nitrogen-fixing bacterial guilds particularly in saline soil ecosystems. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  13. Developing Applications in the Era of Cloud-based SaaS Library Systems

    Directory of Open Access Journals (Sweden)

    Josh Weisman

    2014-10-01

    Full Text Available As the move to cloud-based SaaS library systems accelerates, we must consider what it means to develop applications when the core of the system isn't under the library's control. The entire application lifecycle is changing, from development to testing to production. Developing applications for cloud solutions raises new concerns, such as security, multi-tenancy, latency, and analytics. In this article, we review the landscape and suggest a view of how to be successful for the benefit of library staff and end-users in this new reality. We discuss what kinds of APIs and protocols vendors should be supporting, and suggest how best to take advantage of the innovations being introduced.

  14. ADS-Lib/V1.0. A test library for Accelerator Driven Systems. Summary documentation

    International Nuclear Information System (INIS)

    Lopez Aldama, D.; Trkov, A.

    2005-08-01

    The report describes the generation of a test library for a number of code systems used in the analysis of Accelerator Driven Systems (ADS). The generation of the ADS library was undertaken by IAEA-NDS and the data files are available to users at http://wwwnds. iaea.org/ads/ and also as CD-ROM (upon request).The source of the evaluated nuclear data was the JEFF-3.1 library. Processing was carried out using NJOY-99.90 with the local updates at IAEA-NDS. The resulting processed files are available in ACE format for MCNP and in MATXS format for multi-group transport calculations. (author)

  15. Design and Implementation of a Library and Information Science Open Access Journal Union Catalogue System

    Directory of Open Access Journals (Sweden)

    Sinn-Cheng Lin

    2017-03-01

    Full Text Available Open access is a mode of academic communication that has been on the rise in recent years, but open access academic resources are widely dispersed across the internet, making it occasionally inconvenient in terms of its use. This research is focused on library and information science, using the OAIS reference model as the system framework, two open access platform, DOAJ and E-LIS as the data sources, and through system implementation develop a “library and information science open access journal union catalogue” system. Using the OAI-PMH protocol as the data interoperability standard, and LAMP as the development environment, four major functionalities: injest, archiving, management and access of information were designed, developed, and integrated into system build. Actual testing and verification showed this system is able to successfully collect data from DOAJ and E-LIS open journal resources related to library and information science. The system is now active and functional, and can be used by researchers in the library and science information field.

  16. The value of Web-based library services at Cedars-Sinai Health System.

    Science.gov (United States)

    Halub, L P

    1999-07-01

    Cedars-Sinai Medical Library/Information Center has maintained Web-based services since 1995 on the Cedars-Sinai Health System network. In that time, the librarians have found the provision of Web-based services to be a very worthwhile endeavor. Library users value the services that they access from their desktops because the services save time. They also appreciate being able to access services at their convenience, without restriction by the library's hours of operation. The library values its Web site because it brings increased visibility within the health system, and it enables library staff to expand services when budget restrictions have forced reduced hours of operation. In creating and maintaining the information center Web site, the librarians have learned the following lessons: consider the design carefully; offer what services you can, but weigh the advantages of providing the services against the time required to maintain them; make the content as accessible as possible; promote your Web site; and make friends in other departments, especially information services.

  17. Immunoresponse of Coho salmon immunized with a gene expression library from Piscirickettsia salmonis

    Directory of Open Access Journals (Sweden)

    ALVARO MIQUEL

    2003-01-01

    Full Text Available We have used the expression library immunization technology to study the protection of Coho salmon Oncorhynchus kisutch to the infection with Piscirickettsia salmonis. Purified DNA from this bacterium was sonicated and the fragments were cloned in the expression vector pCMV-Bios. Two libraries were obtained containing 22,000 and 28,000 colonies and corresponding to approximately 8 and 10 times the genome of the pathogen, respectively. On average, the size of the inserts ranged between 300 and 1,000 bp. The plasmid DNA isolated from one of these libraries was purified and 20 µg were injected intramuscularly into 60 fish followed by a second dose of 10 µg applied 40 days later. As control, fish were injected with the same amount of DNA of the vector pCMV-Bios without insert. The titer of IgM anti-P. salmonis of vaccinated fish, evaluated 60 days post-injection, was significantly higher than that of the control group injected with the vector alone. Moreover, this response was specific against P. salmonis antigens, since no cross reaction was detected with Renibacterium salmoninarum and Yersinia ruckeri. The vaccinated and control fish were challenged 60 days after the second dose of DNA with 2.5 x 10(7 P. salmonis corresponding to 7.5 times the LD50. At 30 days post-challenge, 100% mortality was obtained with the control fish while 20% of the vaccinated animals survived. All surviving fish exhibited a lower bacterial load in the kidney than control fish. The expression library was also tested in Balb/c mice and it was found that the humoral immune response was specific to P. salmonis and it was dependent on the amount of DNA injected

  18. Use of data libraries in dosimetry control systems

    International Nuclear Information System (INIS)

    Babenko, V.V.; Babenko, M.I.; Kazimirov, A.S.

    2002-01-01

    Analysis, prediction and planning of dose loads, adequacy in dose management of personnel, evaluation of expediency and sufficiency of existing radiation protection system can be realized with the help of database system of dosimetry control in 'Ukrytie'-shelter

  19. User and system considerations for the TCSTEK software library

    International Nuclear Information System (INIS)

    Gray, W.H.

    1979-08-01

    This report documents the idiosyncrasies of the Tektronix PLOT 10 Terminal Control System level 3.3 software as it currently exists on the ORNL Fusion Energy Division DECsystem-10 computer. It is intended to serve as a reference for future Terminal Control System updates in order that continuity between releases of Terminal Control System PLOT 10 software may be maintained

  20. User and system considerations for the TCSTEK software library

    Energy Technology Data Exchange (ETDEWEB)

    Gray, W.H.

    1979-08-01

    This report documents the idiosyncrasies of the Tektronix PLOT 10 Terminal Control System level 3.3 software as it currently exists on the ORNL Fusion Energy Division DECsystem-10 computer. It is intended to serve as a reference for future Terminal Control System updates in order that continuity between releases of Terminal Control System PLOT 10 software may be maintained.

  1. Mammalian cDNA Library from the NIH Mammalian Gene Collection (MGC) | Office of Cancer Genomics

    Science.gov (United States)

    The MGC provides the research community full-length clones for most of the defined (as of 2006) human and mouse genes, along with selected clones of cow and rat genes. Clones were designed to allow easy transfer of the ORF sequences into nearly any type of expression vector. MGC provides protein ‘expression-ready’ clones for each of the included human genes. MGC is part of the ORFeome Collaboration (OC).

  2. One-Pot Parallel Synthesis of Lipid Library via Thiolactone Ring Opening and Screening for Gene Delivery.

    Science.gov (United States)

    Molla, Mijanur R; Böser, Alexander; Rana, Akshita; Schwarz, Karina; Levkin, Pavel A

    2018-04-18

    Efficient delivery of nucleic acids into cells is of great interest in the field of cell biology and gene therapy. Despite a lot of research, transfection efficiency and structural diversity of gene-delivery vectors are still limited. A better understanding of the structure-function relationship of gene delivery vectors is also essential for the design of novel and intelligent delivery vectors, efficient in "difficult-to-transfect" cells and in vivo clinical applications. Most of the existing strategies for the synthesis of gene-delivery vectors require multiple steps and lengthy procedures. Here, we demonstrate a facile, three-component one-pot synthesis of a combinatorial library of 288 structurally diverse lipid-like molecules termed "lipidoids" via a thiolactone ring opening reaction. This strategy introduces the possibility to synthesize lipidoids with hydrophobic tails containing both unsaturated bonds and reducible disulfide groups. The whole synthesis and purification are convenient, extremely fast, and can be accomplished within a few hours. Screening of the produced lipidoids using HEK293T cells without addition of helper lipids resulted in identification of highly stable liposomes demonstrating ∼95% transfection efficiency with low toxicity.

  3. The Digital Library for Earth System Education: A Progress Report from the DLESE Program Center

    Science.gov (United States)

    Marlino, M. R.; Sumner, T. R.; Kelly, K. K.; Wright, M.

    2002-12-01

    DLESE is a community-owned and governed digital library offering easy access to high quality electronic resources about the Earth system at all educational levels. Currently in its third year of development and operation, DLESE resources are designed to support systemic educational reform, and include web-based teaching resources, tools, and services for the inclusion of data in classroom activities, as well as a "virtual community center" that supports community goals and growth. "Community-owned" and "community-governed" embody the singularity of DLESE through its unique participatory approach to both library building and governance. DLESE is guided by policy development vested in the DLESE Steering Committee, and informed by Standing Committees centered on Collections, Services, Technology, and Users, and community working groups covering a wide variety of interest areas. This presentation highlights both current and projected status of the library and opportunities for community engagement. It is specifically structured to engage community members in the design of the next version of the library release. The current Version 1.0 of the library consists of a web-accessible graphical user interface connected to a database of catalogued educational resources (approximately 3000); a metadata framework enabling resource characterization; a cataloging tool allowing community cataloging and indexing of materials; a search and discovery system allowing browsing based on topic, grade level, and resource type, and permitting keyword and controlled vocabulary-based searches; and a portal website supporting library use, community action, and DLESE partnerships. Future stages of library development will focus on enhanced community collaborative support; development of controlled vocabularies; collections building and community review systems; resource discovery integrating the National Science Education Standards and geography standards; Earth system science vocabulary

  4. Construction of CRISPR Libraries for Functional Screening.

    Science.gov (United States)

    Carstens, Carsten P; Felts, Katherine A; Johns, Sarah E

    2018-01-01

    Identification of gene function has been aided by the ability to generate targeted gene knockouts or transcriptional repression using the CRISPR/CAS9 system. Using pooled libraries of guide RNA expression vectors that direct CAS9 to a specific genomic site allows identification of genes that are either enriched or depleted in response to a selection scheme, thus linking the affected gene to the chosen phenotype. The quality of the data generated by the screening is dependent on the quality of the guide RNA delivery library with regards to error rates and especially evenness of distribution of the guides. Here, we describe a method for constructing complex plasmid libraries based on pooled designed oligomers with high representation and tight distributions. The procedure allows construction of plasmid libraries of >60,000 members with a 95th/5th percentile ratio of less than 3.5.

  5. Design and Development of a University E-Library System in Turkey: A Case from Dicle University

    Directory of Open Access Journals (Sweden)

    Murat YALMAN

    2014-08-01

    Full Text Available Services provided for students in traditional libraries at universities have been renovated in line with the development of technology. Therefore, today libraries are not evaluated with their sizes of places but with the variety and abundance of sources found in libraries. In terms of current educational activities, it is fairly important for universities to structure and renovate their libraries. The reason is that learning is now independent of place and time. The spread of the Internet and of Internet technologies and the increase in the number of Internet users make e-library obligatory and unavoidable. Therefore, universities should internalize the concept of e-library and take electronic library into consideration while restructuring their library services. The present study tried to determine how to carry out an e-library design in accordance with the library services of universities. In this process, the overall purpose was to determine the needs by examining the user interface of the webpage designed, its interface features, its differences, ease of its use, its clarity for users to understand and the pros and cons of the system. In this way, universities using e-library are believed to provide their students with better services by increasing their quality of education.

  6. “Clicking” with your audience: Evaluating the use of personal response systems in library instruction

    Directory of Open Access Journals (Sweden)

    Emily K. Chan

    2011-03-01

    Full Text Available University of the Pacific librarians used personal response systems (PRS or clickers in first-year mandatory library instructional sessions to assess their effects on student engagement and retention of learning outcomes. Students who utilized clickers during their library session reported greater enjoyment and encouragement to participate (n=291. Students in the sessions not utilizing the clickers achieved better learning outcomes than their counterparts who utilized clickers (n=326. The implications of these results are discussed, specifically within the context of pedagogy and tailoring instruction to the Millennial generation.

  7. ThermoCycle: A Modelica library for the simulation of thermodynamic systems

    DEFF Research Database (Denmark)

    Quoilin, Sylvain; Desideri, Adriano; Wronski, Jorrit

    2014-01-01

    This paper presents the results of an on-going project to develop ThermoCycle, an open Modelica library for the simulation of low-capacity thermodynamic cycles and thermal systems. Special attention is paid to robustness and simulation speed since dynamic simulations are often limited by numerical...... constraints and failures, either during initialization or during integration. Furthermore, the use of complex equations of state (EOS) to compute thermodynamic properties significantly decreases the simulation speed. In this paper, the approach adopted in the library to overcome these challenges is presented...

  8. Environmental Protection Agency Library System Book Catalog. Holdings from August 1973 to December 1974

    International Nuclear Information System (INIS)

    1975-01-01

    The U.S. Environmental Protection Agency Library System Book Catalog of holdings from August 1973 to December 1974 has been published in a single volume. The full catalog lists alphabetically by title the complete entry for each book owned by the individual EPA libraries. The indexes to the Book Catalog are in two parts. Part 1, the Author Index, lists each author in alphabetical order. The Subject Index (Part 2) lists, in alphabetical order, the subject headings assigned to the books in the catalog

  9. Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.

    Science.gov (United States)

    Quail, Michael A; Gu, Yong; Swerdlow, Harold; Mayho, Matthew

    2012-12-01

    Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Mapping of polyketide biosynthesis pathways in Aspergillus nidulans using a genome wide PKS gene deletion library

    DEFF Research Database (Denmark)

    Larsen, Thomas Ostenfeld; Rank, Christian; Klejnstrup, Marie Louise

    In order to map new links between PKS genes and their products in Aspergillus nidulans we have systematically deleted all thirty-two individual genes predicted to encode polyketide synthases in this model organism. This number greatly exceeds the number of currently known PKs calling for new appr...

  11. AN Integrated Bibliographic Information System: Concept and Application for Resource Sharing in Special Libraries

    Science.gov (United States)

    1987-05-01

    workload (beyond that of say an equivalent academic or corporate technical libary ) for the Defense Department libraries. Figure 9 illustrates the range...summer. The hardware configuration for the system is as follows: " Digital Equipment Corporation VAX 11/750 central processor with 6 mega- bytes of real

  12. An Examination of Electronic Links between the Book Trade and Library Acquisitions Systems.

    Science.gov (United States)

    Bonk, Sharon C.

    Based on interviews and correspondence conducted during 1981, this report describes British library and book trade plans for expansion of their internal order processing systems to include the eventual exchange of data through the use of telecommunications. Comparisons are made between the situation in England and the United States. The report…

  13. Millennial Undergraduate Research Strategies in Web and Library Information Retrieval Systems

    Science.gov (United States)

    Porter, Brandi

    2011-01-01

    This article summarizes the author's dissertation regarding search strategies of millennial undergraduate students in Web and library online information retrieval systems. Millennials bring a unique set of search characteristics and strategies to their research since they have never known a world without the Web. Through the use of search engines,…

  14. 2006 Automation Survey: The Systems Are Changing. But School Libraries Aren't

    Science.gov (United States)

    Fuller, Daniel

    2006-01-01

    This article presents the findings of the 2006 School Library Journal-San Jose State University Automation Survey. The study takes a close look at the systems that media specialists are using, how they are using them, and what librarians want from their future automation programs. The findings reveal that while respondents were satisfied with…

  15. The design of a web – based integrated library system with internet ...

    African Journals Online (AJOL)

    Developing countries like Nigeria face series of defects in developing, managing and securing an Integrated Library System (ILS) in tertiary institutions and Secondary Schools where they are mostly needed. ILSs face issues such as non – interactive, speed, cost, unavailability of experienced users and programmers and ...

  16. Creating Better Library Information Systems: The Road to FRBR-Land

    Science.gov (United States)

    Mercun, Tanja; Švab, Katarina; Harej, Viktor; Žumer, Maja

    2013-01-01

    Introduction: To provide valuable services in the future, libraries will need to create better information systems and set up an infrastructure more in line with the current technologies. The "Functional Requirements for Bibliographic Records" conceptual model provides a basis for this transformation, but there are still a number of…

  17. A Complex Systems Framework for Research on Leadership and Organizational Dynamics in Academic Libraries

    Science.gov (United States)

    Gilstrap, Donald L.

    2009-01-01

    This article provides a historiographical analysis of major leadership and organizational development theories that have shaped our thinking about how we lead and administrate academic libraries. Drawing from behavioral, cognitive, systems, and complexity theories, this article discusses major theorists and research studies appearing over the past…

  18. Library generation and tests for the HAMMER system from the ENDF/B-IV data

    International Nuclear Information System (INIS)

    Queiroz Bogado Leite, S. de; Chalhoub, E.S.; Moraes, Marisa de.

    1983-05-01

    The modifications made to a number of programs belonging to the HAMMER system in order to process data from ENDF/B-IV are presented. An alternate scheme of representing the resonance region by means of extensive tabulation of profiles is made available for selected materials. Numerical examples illustrate comparisons of the results obtained with these libraries against those from the literature. (Author) [pt

  19. Implementing of AMPX-II system for a univac computer neutron cross-section libraries

    International Nuclear Information System (INIS)

    Sancho, J.; Verdu, G.; Serradell, V.

    1984-01-01

    The AMPX-II system, developed at ORNL, is constituted by a modular set of computer programs, for generation and handling of several nuclear data libraries. The processing starts from ENDF/B library. Along this paper, we refer mainly to the modules related with neutron cross section libraries: master, working and weighted. These modules have been implemented recently for a UNIVAC 1100/60 computer in the Universidad Politecnica de Valencia (Spain). In order to run the programs in that machine it has been necessary to introduce a number of modifications into their programing structure. The main difficulties found in this work and the need of verification for the new versions are also pointed out. We also refer to the results obtained from the execution of a set of little sample problems. (author)

  20. Development of digital library system on regulatory documents for nuclear power plants

    International Nuclear Information System (INIS)

    Lee, K. H.; Kim, K. J.; Yoon, Y. H.; Kim, M. W.; Lee, J. I.

    2001-01-01

    The main objective of this study is to establish nuclear regulatory document retrieval system based on internet. With the advancement of internet and information processing technology, information management patterns are going through a new paradigm. Getting along the current of the time, it is general tendency to transfer paper-type documents into electronic-type documents through document scanning and indexing. This system consists of nuclear regulatory documents, nuclear safety documents, digital library, and information system with index and full text

  1. Network communication libraries for the next control system of the KEK e-/e+ Linac

    International Nuclear Information System (INIS)

    Kamikubota, Norihiko; Furukawa, Kazuro; Nakahara, Kazuo; Abe, Isamu

    1992-01-01

    The network communication libraries for the next control system of the KEK Linac have been developed. They are based on TCP/IP sockets, and show high availability among the different operating systems: UNIX, VAX/VMS, and MS-DOS. They also show high source portability of application programs among the different computer systems provided by various vendors. The performance and problems are presented in detail. (author)

  2. Development of in vitro transposon assisted signal sequence trapping and its use in screening Bacillus halodurans C125 and Sulfolobus solfataricus P2 gene libraries

    DEFF Research Database (Denmark)

    Becker, F.; Schnorr, K.; Wilting, R.

    2004-01-01

    To identify genes encoding extracytosolic proteins, a minitransposon, TnSig, containing a signal-less beta-lactamase ('bla) as reporter gene, was constructed and used for in vitro transposition of genomic libraries made in Escherichia coli. The 'bla gene was cloned into a bacteriophage MU...... minitransposon enabling translational fusions between 'bla and target genes. Fusion of TnSig in the correct reading frame to a protein carrying transmembrane domains or signal peptides resulted in ampicillin resistance of the corresponding clone. Prokaryotic gene libraries from the alkaliphilic bacterium...... Bacillus halodurans C125 and the hyperthermophilic archaeon Sulfolobus solfataricus P2 were tagged with TnSig. The genomic sequences, which are publicly available (EMBL BA000004 and EMBL AE006641), were used for rapid open reading frame (ORF) identification and prediction of protein localisation...

  3. Discover Earth: An earth system science program for libraries and their communities

    Science.gov (United States)

    Curtis, L.; Dusenbery, P.

    2010-12-01

    The view from space has deepened our understanding of Earth as a global, dynamic system. Instruments on satellites and spacecraft, coupled with advances in ground-based research, have provided us with astonishing new perspectives of our planet. Now more than ever, enhancing the public’s understanding of Earth’s physical and biological systems is vital to helping citizens make informed policy decisions especially when they are faced with the consequences of global climate change. In spite of this relevance, there are many obstacles to achieving broad public understanding of key earth system science (ESS) concepts. Strategies for addressing climate change can only succeed with the full engagement of the general public. As reported by U.S. News and World Report in 2010, small towns in rural America are emerging as the front line in the climate change debate in the country. The Space Science Institute’s National Center for Interactive Learning (NCIL) in partnership with the American Library Association (ALA), the Lunar and Planetary Institute (LPI), and the National Girls Collaborative Project (NGCP) have received funding from NSF to develop a national project called the STAR Library Education Network: a hands-on learning program for libraries and their communities (or STAR-Net for short). STAR stands for Science-Technology, Activities and Resources. There are two distinct components of STAR-Net: Discover Earth and Discover Tech. While the focus for education reform is on school improvement, there is considerable research that supports the role that out-of-school experiences can play in student achievement. Libraries provide an untapped resource for engaging underserved youth and their families in fostering an appreciation and deeper understanding of science and technology topics. The overarching goal of the project is to reach underserved youth and their families with informal STEM learning experiences. The Discover Earth part of STAR_Net will produce ESS

  4. Automated Periodicals System at a Community College Library

    Directory of Open Access Journals (Sweden)

    Vivian Harp

    1974-06-01

    Full Text Available Automated systems need not be extensive to save time and improve efficiency. Moraine Valley's off-line operation, based on a file of 715 periodical titles, generates renewal orders, sends claims, and records subscription histories.

  5. RAMAS: The RITL Automated Management System. Master Control and Periodicals Control Subsystems. Stockholm Papers in Library and Information Science.

    Science.gov (United States)

    Ya-chun, Lian

    An automated minicomputer-based library management system is being developed at the Swedish Royal Institute of Technology Library (RITL). RAMAS (the RITL Automated Management System) currently deals with periodical check-in, claiming, index-handling, and binding control. A RAMAS bibliographic record can be accessed from eight different points…

  6. Embedded Library Guides in Learning Management Systems Help Students Get Started on Research Assignments

    Directory of Open Access Journals (Sweden)

    Dominique Daniel

    2016-03-01

    Full Text Available Objective – To determine whether library guides embedded in learning management systems (LMS get used by students, and to identify best practices for the creation and promotion of these guides by librarians. Design – Mixed methods combining quantitative and qualitative data collection and analysis (survey, interviews, and statistical analysis. Setting – A large public university in the United States of America. Subjects – 100 undergraduate students and 14 librarians. Methods – The researchers surveyed undergraduate students who were participating in a Project Information Literacy study about their use of library guides in the learning management system (LMS for a given quarter. At that university, all course pages in the LMS are automatically assigned a library guide. In addition, web usage data about the course-embedded guides was analyzed and high use guides were identified, namely guides that received an average of at least two visits per student enrolled in a course. The researchers also conducted a qualitative analysis of the layout of the high use guides, including the number of widgets (or boxes and links. Finally, librarians who created high use library guides were interviewed. These mixed methods were designed to address four research questions: 1 Were students finding the guides in the LMS, and did they find the guides useful? 2 Did high use guides differ in design and composition? 3 Were the guides designed for a specific course, or for an entire department or college? and, 4 How did the librarians promote use? Main Results – Only 33% of the students said they noticed the library guide in the LMS course page, and 21% reported using the guide. Among those who used the guide, the majority were freshmen (possibly because embedding of library guides in the LMS had just started at the university. Library guides with high use in relation to class enrollment did not significantly differ from low use guides in terms of numbers of

  7. Functional study and regional mapping of 44 hormono-regulated genes isolated from a porcine granulosa cell library

    Directory of Open Access Journals (Sweden)

    Hatey François

    2001-01-01

    Full Text Available Abstract cDNA clones from a pig granulosa cell cDNA library were isolated by differential hybridisation for follicle stimulating hormone (FSH regulation in granulosa cells in a previous study. The clones that did not match any known sequence were studied for their expression in granulosa cells (treated or not by FSH and in fresh isolated ovarian follicles mainly by comparative RT-PCR analysis. These results give functional data on genes that may be implicated in follicular growing. These ESTs have been localised on the porcine genome, using a somatic cell hybrid panel, providing new type I markers on the porcine map and information on the comparative map between humans and pigs.

  8. MINPACK-1, Subroutine Library for Nonlinear Equation System

    International Nuclear Information System (INIS)

    Garbow, Burton S.

    1984-01-01

    1 - Description of problem or function: MINPACK1 is a package of FORTRAN subprograms for the numerical solution of systems of non- linear equations and nonlinear least-squares problems. The individual programs are: Identification/Description: - CHKDER: Check gradients for consistency with functions, - DOGLEG: Determine combination of Gauss-Newton and gradient directions, - DPMPAR: Provide double precision machine parameters, - ENORM: Calculate Euclidean norm of vector, - FDJAC1: Calculate difference approximation to Jacobian (nonlinear equations), - FDJAC2: Calculate difference approximation to Jacobian (least squares), - HYBRD: Solve system of nonlinear equations (approximate Jacobian), - HYBRD1: Easy-to-use driver for HYBRD, - HYBRJ: Solve system of nonlinear equations (analytic Jacobian), - HYBRJ1: Easy-to-use driver for HYBRJ, - LMDER: Solve nonlinear least squares problem (analytic Jacobian), - LMDER1: Easy-to-use driver for LMDER, - LMDIF: Solve nonlinear least squares problem (approximate Jacobian), - LMDIF1: Easy-to-use driver for LMDIF, - LMPAR: Determine Levenberg-Marquardt parameter - LMSTR: Solve nonlinear least squares problem (analytic Jacobian, storage conserving), - LMSTR1: Easy-to-use driver for LMSTR, - QFORM: Accumulate orthogonal matrix from QR factorization QRFAC Compute QR factorization of rectangular matrix, - QRSOLV: Complete solution of least squares problem, - RWUPDT: Update QR factorization after row addition, - R1MPYQ: Apply orthogonal transformations from QR factorization, - R1UPDT: Update QR factorization after rank-1 addition, - SPMPAR: Provide single precision machine parameters. 4. Method of solution - MINPACK1 uses the modified Powell hybrid method and the Levenberg-Marquardt algorithm

  9. Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library

    Directory of Open Access Journals (Sweden)

    Tatsuro Koyanagi

    2010-05-01

    Full Text Available Background: Peri-implantitis (PI is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. The subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in PI, the microbiota responsible for PI has not been identified. Objective: The purpose of this study was to identify the microbiota in subjects who have PI, clinically healthy implants, and periodontitis-affected teeth using 16S rRNA gene clone library analysis to clarify the microbial differences. Design: Three subjects participated in this study. The conditions around the teeth and implants were evaluated based on clinical and radiographic examinations and diseased implants, clinically healthy implants, and periodontally diseased teeth were selected. Subgingival plaque samples were taken from the deepest pockets using sterile paper points. Prevalence and identity of bacteria was analyzed using a 16S rRNA gene clone library technique. Results: A total of 112 different species were identified from 335 clones sequenced. Among the 112 species, 51 (46% were uncultivated phylotypes, of which 22 were novel phylotypes. The numbers of bacterial species identified at the sites of PI, periodontitis, and periodontally healthy implants were 77, 57, and 12, respectively. Microbiota in PI mainly included Gram-negative species and the composition was more diverse when compared to that of the healthy implant and periodontitis. The phyla Chloroflexi, Tenericutes, and Synergistetes were only detected at PI sites, as were Parvimonas micra, Peptostreptococcus stomatis, Pseudoramibacter alactolyticus, and Solobacterium moorei. Low levels of periodontopathic bacteria, such as Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, were seen in peri-implant lesions. Conclusions: The biofilm in PI showed a more complex microbiota when compared to periodontitis and

  10. Identification of aminoglycoside and β-lactam resistance genes from within an infant gut functional metagenomic library.

    Directory of Open Access Journals (Sweden)

    Fiona Fouhy

    Full Text Available The infant gut microbiota develops rapidly during the first 2 years of life, acquiring microorganisms from diverse sources. During this time, significant opportunities exist for the infant to acquire antibiotic resistant bacteria, which can become established and constitute the infant gut resistome. With increased antibiotic resistance limiting our ability to treat bacterial infections, investigations into resistance reservoirs are highly pertinent. This study aimed to explore the nascent resistome in antibiotically-naïve infant gut microbiomes, using a combination of metagenomic approaches. Faecal samples from 22 six-month-old infants without previous antibiotic exposure were used to construct a pooled metagenomic library, which was functionally screened for ampicillin and gentamicin resistance. Our library of ∼220Mb contained 0.45 ampicillin resistant hits/Mb and 0.059 gentamicin resistant hits/Mb. PCR-based analysis of fosmid clones and uncloned metagenomic DNA, revealed a diverse and abundant aminoglycoside and β-lactam resistance reservoir within the infant gut, with resistance determinants exhibiting homology to those found in common gut inhabitants, including Escherichia coli, Enterococcus sp., and Clostridium difficile, as well as to genes from cryptic environmental bacteria. Notably, the genes identified differed from those revealed when a sequence-driven PCR-based screen of metagenomic DNA was employed. Carriage of these antibiotic resistance determinants conferred substantial, but varied (2-512x, increases in antibiotic resistance to their bacterial host. These data provide insights into the infant gut resistome, revealing the presence of a varied aminoglycoside and β-lactam resistance reservoir even in the absence of selective pressure, confirming the infant resistome establishes early in life, perhaps even at birth.

  11. User Characteristics、User Involvement and Technology Acceptance in Library Automation System Usage

    Directory of Open Access Journals (Sweden)

    Piching Chu

    2002-12-01

    Full Text Available This paper looks into the relationship of user characteristics, user involvement and technology acceptance, also try to find the relationship of the above three variables and library automation system usage. By interview and questionnaire study, the results are described, according to the analysis of the relative literature and the main conclusions, the researcher offers some suggestions for improving the system usage.[Article content in Chinese

  12. Selecting a Web Content Management System for an Academic Library Website

    Directory of Open Access Journals (Sweden)

    Elizabeth L. Black

    2011-12-01

    Full Text Available This article describes the selection of a web content management system (CMS at the Ohio State University Libraries. The author outlines the need for a CMS, describes the system requirements to support a large distributed content model and shares the CMS trial method used, which directly included content provider feedback side-by-side with the technical experts. The selected CMS is briefly described.

  13. The Open Library System — Re-invented, Implemented and Working

    Directory of Open Access Journals (Sweden)

    Jens Hofman Hansen

    2011-02-01

    Full Text Available A highly modularised and open system is described in this paper, and its ability to support the library’s strategy. By focusing on a single problem, it is possible to solve it in a qualitatively or quantitatively different way that is dictated by business vision and user needs. This results in a highly innovative library system when seen as a whole.

  14. Computer program user's manual for FIREFINDER digital topographic data verification library dubbing system

    Science.gov (United States)

    Ceres, M.; Heselton, L. R., III

    1981-11-01

    This manual describes the computer programs for the FIREFINDER Digital Topographic Data Verification-Library-Dubbing System (FFDTDVLDS), and will assist in the maintenance of these programs. The manual contains detailed flow diagrams and associated descriptions for each computer program routine and subroutine. Complete computer program listings are also included. This information should be used when changes are made in the computer programs. The operating system has been designed to minimize operator intervention.

  15. PCS a code system for generating production cross section libraries

    International Nuclear Information System (INIS)

    Cox, L.J.

    1997-01-01

    This document outlines the use of the PCS Code System. It summarizes the execution process for generating FORMAT2000 production cross section files from FORMAT2000 reaction cross section files. It also describes the process of assembling the ASCII versions of the high energy production files made from ENDL and Mark Chadwick's calculations. Descriptions of the function of each code along with its input and output and use are given. This document is under construction. Please submit entries, suggestions, questions, and corrections to (ljc at sign llnl.gov) 3 tabs

  16. The FOND-2.2 evaluated neutron data library (Russian library of evaluated neutron data files for generating sets of constants in the ABBN constants system)

    International Nuclear Information System (INIS)

    Koshcheev, V.N.; Nikolaev, M.N.; Korchagina, Zh.A.; Savoskina, G.V.

    2001-01-01

    A short description is given of the Russian evaluated neutron data library FOND-2.2. The main purpose of FOND-2.2 is to provide sets of constants for the ABBN constants system. A history of its compilation and the sources of the neutron data are given. The contents of FOND-2.2 are presented with brief comments. (author)

  17. A Study of Assessment of Library E-resource Metasearch System

    Directory of Open Access Journals (Sweden)

    Fan-Chuan Tseng

    2008-12-01

    Full Text Available As the emergent development of computer and network technology, digital library and information service has become an important approach for users to increase their academic as well as job performance due to the authorized, accurate, and reliable information. In the realm of information management, the exploration of factors influencing information system utilization and the evaluation of benefits are two critical issues. However, related studies focus more on the system functions and usually ignore the effects of individual cognition and social influence. In this study, we extend the IS success model with two additional factors-self-efficacy and subjective norm, trying to explore the potential influences on digital library usage, user satisfaction and performance.This research takes the Chang Jung Christian University library E-resources Metasearch system as the case study. A questionnaire was developed and finally got 160 useful student samples to test and verify the hypotheses. The research results confirmed that self-efficacy and subjective norm can significantly affect the usage of E-resource Metasearch System. Subsequently, the users feel satisfied with the system, and increase their academic performance. Finally, we provide discussion, implications and suggestions in two aspects – cooperating more with educational programs to increase users’ information literacy, and improving the customer-oriented system interface, functions, and services to enhance its quality. [Article content in Chinese

  18. Automating the Small Library.

    Science.gov (United States)

    Skapura, Robert

    1987-01-01

    Discusses the use of microcomputers for automating school libraries, both for entire systems and for specific library tasks. Highlights include available library management software, newsletters that evaluate software, constructing an evaluation matrix, steps to consider in library automation, and a brief discussion of computerized card catalogs.…

  19. Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole-Genome Bacterial Artificial Chromosome Library in Streptomyces spp.

    Science.gov (United States)

    Xu, Min; Wang, Yemin; Zhao, Zhilong; Gao, Guixi; Huang, Sheng-Xiong; Kang, Qianjin; He, Xinyi; Lin, Shuangjun; Pang, Xiuhua; Deng, Zixin

    2016-01-01

    ABSTRACT Genome sequencing projects in the last decade revealed numerous cryptic biosynthetic pathways for unknown secondary metabolites in microbes, revitalizing drug discovery from microbial metabolites by approaches called genome mining. In this work, we developed a heterologous expression and functional screening approach for genome mining from genomic bacterial artificial chromosome (BAC) libraries in Streptomyces spp. We demonstrate mining from a strain of Streptomyces rochei, which is known to produce streptothricins and borrelidin, by expressing its BAC library in the surrogate host Streptomyces lividans SBT5, and screening for antimicrobial activity. In addition to the successful capture of the streptothricin and borrelidin biosynthetic gene clusters, we discovered two novel linear lipopeptides and their corresponding biosynthetic gene cluster, as well as a novel cryptic gene cluster for an unknown antibiotic from S. rochei. This high-throughput functional genome mining approach can be easily applied to other streptomycetes, and it is very suitable for the large-scale screening of genomic BAC libraries for bioactive natural products and the corresponding biosynthetic pathways. IMPORTANCE Microbial genomes encode numerous cryptic biosynthetic gene clusters for unknown small metabolites with potential biological activities. Several genome mining approaches have been developed to activate and bring these cryptic metabolites to biological tests for future drug discovery. Previous sequence-guided procedures relied on bioinformatic analysis to predict potentially interesting biosynthetic gene clusters. In this study, we describe an efficient approach based on heterologous expression and functional screening of a whole-genome library for the mining of bioactive metabolites from Streptomyces. The usefulness of this function-driven approach was demonstrated by the capture of four large biosynthetic gene clusters for metabolites of various chemical types, including

  20. Development of a common nuclear group constants library system: JSSTDL-295n-104γ based on JENDL-3 nuclear data library

    International Nuclear Information System (INIS)

    Hasegawa, A.

    1992-01-01

    JSSTDL 295n-104γ: A common group cross-section library system has been developed in JAERI to be used in fairly wide range of applications in nuclear industry. This system is composed of a common 295n-104γ group cross-section library based on JENDL-3 nuclear data file and its utility codes. Target of this system is focused to the criticality or shielding calculations in fast and fusion reactors using ANISN, DOT, or MORSE code. Specifications of the common group constants were decided responding to the request from various nuclear data users, particularly from nuclear design group in Japan. Group structure is decided so as to cover almost all group structures currently used in our country. This library includes self-shielding factor tables for primary reactions. A routine for generating macro-scopic cross-section using the self-shielding factor table is also provided. Neutron cross-sections and photon production cross-sections are processed by Prof. GROUCH-G/B code system and γ ray transport cross-sections are generated by GAMLEG-JR. In this paper, outline and present status of the JSSTDL library system is described along with two examples adopted in JENDL-3 benchmark test. One is for shielding calculation, where effects of self-shielding factor (f-table) is shown in conjunction with the analysis of the ASPIS natural iron deep penetration experiment. Without considering resonance self-shielding effect in resonance energy region for resonant nuclides like iron, the results is completely missled in the attenuation profile calculation in the shields. The other example is fast rector criticality calculations of very small critical assemblies with very high enrichment fuel materials where some basic characteristics of this library is presented. (orig.)

  1. Gene-gene interactions and gene polymorphisms of VEGFA and EG-VEGF gene systems in recurrent pregnancy loss.

    Science.gov (United States)

    Su, Mei-Tsz; Lin, Sheng-Hsiang; Chen, Yi-Chi; Kuo, Pao-Lin

    2014-06-01

    Both vascular endothelial growth factor A (VEGFA) and endocrine gland-derived vascular endothelial growth factor (EG-VEGF) systems play major roles in angiogenesis. A body of evidence suggests VEGFs regulate critical processes during pregnancy and have been associated with recurrent pregnancy loss (RPL). However, little information is available regarding the interaction of these two major major angiogenesis-related systems in early human pregnancy. This study was conducted to investigate the association of gene polymorphisms and gene-gene interaction among genes in VEGFA and EG-VEGF systems and idiopathic RPL. A total of 98 women with history of idiopathic RPL and 142 controls were included, and 5 functional SNPs selected from VEGFA, KDR, EG-VEGF (PROK1), PROKR1 and PROKR2 were genotyped. We used multifactor dimensionality reduction (MDR) analysis to choose a best model and evaluate gene-gene interactions. Ingenuity pathways analysis (IPA) was introduced to explore possible complex interactions. Two receptor gene polymorphisms [KDR (Q472H) and PROKR2 (V331M)] were significantly associated with idiopathic RPL (P<0.01). The MDR test revealed that the KDR (Q472H) polymorphism was the best loci to be associated with RPL (P=0.02). IPA revealed EG-VEGF and VEGFA systems shared several canonical signaling pathways that may contribute to gene-gene interactions, including the Akt, IL-8, EGFR, MAPK, SRC, VHL, HIF-1A and STAT3 signaling pathways. Two receptor gene polymorphisms [KDR (Q472H) and PROKR2 (V331M)] were significantly associated with idiopathic RPL. EG-VEGF and VEGFA systems shared several canonical signaling pathways that may contribute to gene-gene interactions, including the Akt, IL-8, EGFR, MAPK, SRC, VHL, HIF-1A and STAT3.

  2. Chemical analysis of a genome wide polyketide synthase gene deletion library in Aspergillus nidulans

    DEFF Research Database (Denmark)

    Larsen, Thomas Ostenfeld; Klejnstrup, Marie Louise; Nielsen, Jakob Blæsbjerg

    . This may reflect that many PKs are either produced in small amounts, under special conditions or in developmental stages that are rarely observed under laboratory conditions. In order to trigger expression of “silent” genes we are currently pursuing several approaches; i) stimulation of A. nidulans wild...

  3. The Construction of cDNA Libraries from Human Single Preimplantation Embryos and Their Use in the Study of Gene Expression During Development

    OpenAIRE

    Adjaye, James; Daniels, Rob; Monk, Marilyn

    1998-01-01

    Purpose:The construction and application of polymerase chain reaction (PCR)-based cDNA libraries from unfertilized human oocytes and single preimplantation-stage embryos are described. The purpose of these studies is to provide a readily available resource for the study of gene expression during human preimplantation development.

  4. 16S rRNA Gene Sequence Analysis of Drinking Water Using RNA and DNA Extracts as Targets for Clone Library Development - Poster

    Science.gov (United States)

    We examined the bacterial composition of chlorinated drinking water using 16S rRNA gene clone libraries derived from RNA and DNA extracted from twelve water samples collected in three different months (June, August, and September of 2007). Phylogenetic analysis of 1234 and 1117 ...

  5. Effective e-Training: Using a Course Management System and e-Learning Tools to Train Library Employees

    Science.gov (United States)

    See, Andrew; Teetor, Travis Stephen

    2014-01-01

    In the summer of 2012, the University of Arizona Libraries implemented an online training program to effectively train Access Services staff and student employees at a large academic research library. This article discusses the program, which was built using a course management system (D2L) and various e-Learning software applications (Articulate…

  6. IDENTIFICATION OF ACTIVE BACTERIAL COMMUNITIES IN A MODEL DRINKING WATER BIOFILM SYSTEM USING 16S RRNA-BASED CLONE LIBRARIES

    Science.gov (United States)

    Recent phylogenetic studies have used DNA as the target molecule for the development of environmental 16S rDNA clone libraries. As DNA may persist in the environment, DNA-based libraries cannot be used to identify metabolically active bacteria in water systems. In this study, a...

  7. Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

    Directory of Open Access Journals (Sweden)

    Gong Xia

    2012-10-01

    Full Text Available Abstract Background Interest in cellulose degrading enzymes has increased in recent years due to the expansion of the cellulosic biofuel industry. The rumen is a highly adapted environment for the degradation of cellulose and a promising source of enzymes for industrial use. To identify cellulase enzymes that may be of such use we have undertaken a functional metagenomic screen to identify cellulase enzymes from the bacterial community in the rumen of a grass-hay fed dairy cow. Results Twenty five clones specifying cellulose activity were identified. Subcloning and sequence analysis of a subset of these hydrolase-positive clones identified 10 endoglucanase genes. Preliminary characterization of the encoded cellulases was carried out using crude extracts of each of the subclones. Zymogram analysis using carboxymethylcellulose as a substrate showed a single positive band for each subclone, confirming that only one functional cellulase gene was present in each. One cellulase gene, designated Cel14b22, was expressed at a high level in Escherichia coli and purified for further characterization. The purified recombinant enzyme showed optimal activity at pH 6.0 and 50°C. It was stable over a broad pH range, from pH 4.0 to 10.0. The activity was significantly enhanced by Mn2+ and dramatically reduced by Fe3+ or Cu2+. The enzyme hydrolyzed a wide range of beta-1,3-, and beta-1,4-linked polysaccharides, with varying activities. Activities toward microcrystalline cellulose and filter paper were relatively high, while the highest activity was toward Oat Gum. Conclusion The present study shows that a functional metagenomic approach can be used to isolate previously uncharacterized cellulases from the rumen environment.

  8. Library Automation.

    Science.gov (United States)

    Husby, Ole

    1990-01-01

    The challenges and potential benefits of automating university libraries are reviewed, with special attention given to cooperative systems. Aspects discussed include database size, the role of the university computer center, storage modes, multi-institutional systems, resource sharing, cooperative system management, networking, and intelligent…

  9. An optimized lentiviral vector system for conditional RNAi and efficient cloning of microRNA embedded short hairpin RNA libraries.

    Science.gov (United States)

    Adams, Felix F; Heckl, Dirk; Hoffmann, Thomas; Talbot, Steven R; Kloos, Arnold; Thol, Felicitas; Heuser, Michael; Zuber, Johannes; Schambach, Axel; Schwarzer, Adrian

    2017-09-01

    RNA interference (RNAi) and CRISPR-Cas9-based screening systems have emerged as powerful and complementary tools to unravel genetic dependencies through systematic gain- and loss-of-function studies. In recent years, a series of technical advances helped to enhance the performance of virally delivered RNAi. For instance, the incorporation of short hairpin RNAs (shRNAs) into endogenous microRNA contexts (shRNAmiRs) allows the use of Tet-regulated promoters for synchronous onset of gene knockdown and precise interrogation of gene dosage effects. However, remaining challenges include lack of efficient cloning strategies, inconsistent knockdown potencies and leaky expression. Here, we present a simple, one-step cloning approach for rapid and efficient cloning of miR-30 shRNAmiR libraries. We combined a human miR-30 backbone retaining native flanking sequences with an optimized all-in-one lentiviral vector system for conditional RNAi to generate a versatile toolbox characterized by higher doxycycline sensitivity, reduced leakiness and enhanced titer. Furthermore, refinement of existing shRNA design rules resulted in substantially improved prediction of powerful shRNAs. Our approach was validated by accurate quantification of the knockdown potency of over 250 single shRNAmiRs. To facilitate access and use by the scientific community, an online tool was developed for the automated design of refined shRNA-coding oligonucleotides ready for cloning into our system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. An Interactive Computer-Based Circulation System for Northwestern University: The Library Puts It to Work

    Directory of Open Access Journals (Sweden)

    Velma Veneziano

    1972-06-01

    Full Text Available Northwestern University Library's on-line circulation system has resulted in dramatic changes in practices and procedures in the Circulation Services Section. After a hectic period of implementation, the staff soon began to adjust to the system. Over the past year and a half, they have devised ways to use the system to maximum advantage, so that manual and machine systems now mesh in close harmony. Freed from time-consuming clerical chores, the staff have been challenged to use their released time to best advantage, with the result that the "service" in "Circulation Services" is much closer to being a reality.

  11. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    Science.gov (United States)

    Nagarkar-Jaiswal, Sonal; Lee, Pei-Tseng; Campbell, Megan E; Chen, Kuchuan; Anguiano-Zarate, Stephanie; Cantu Gutierrez, Manuel; Busby, Theodore; Lin, Wen-Wen; He, Yuchun; Schulze, Karen L; Booth, Benjamin W; Evans-Holm, Martha; Venken, Koen JT; Levis, Robert W; Spradling, Allan C; Hoskins, Roger A; Bellen, Hugo J

    2015-01-01

    Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates. DOI: http://dx.doi.org/10.7554/eLife.05338.001 PMID:25824290

  12. Improvements on burnup chain model and group cross section library in the SRAC system

    International Nuclear Information System (INIS)

    Akie, Hiroshi; Okumura, Keisuke; Takano, Hideki; Ishiguro, Yukio; Kaneko, Kunio.

    1992-01-01

    Data and functions of the cell burnup calculation of the SRAC system were revised to improve mainly the accuracy of the burnup calculation of high conversion light water reactors (HCLWRs). New burnup chain models were developed in order to treat fission products (FPs) and actinide nuclides in detail. Group cross section library, SRACLIB-JENDL2, was generated based on JENDL-2 nuclear data file. In generating this library, emphasis was placed on FPs and actinides. Also revised were the data such as the average energy release per fission for various actinides. These improved data were verified by performing the burnup analysis of PWR spent fuels. Some new functions were added to the SRAC system for the convenience to yield macroscopic cross sections used in the core burnup process. (author)

  13. IoT-Based Library Automation and Monitoring system: Developing an Implementation framework of Implementation

    OpenAIRE

    Bayani, Majid; Segura, Alberto; Alvarado, Marjorie; Loaiza, Mayra

    2018-01-01

    Currently, the Information and Communication Technology (ICT) and related topics such as Internet of Things (IoT) have an essential influence on all elements of human life. IoT as a prevalent phenomenon is transforming daily life by the usage of the smart features of Radio Frequency Identification (RFID) and Wireless Sensor Network (WSN) technologies. As IoT progresses, it has extended in size and dimension, improving many contexts of the society; such as, the traditional library system. This...

  14. Embedded Library Guides in Learning Management Systems Help Students Get Started on Research Assignments

    OpenAIRE

    Dominique Daniel

    2016-01-01

    Objective – To determine whether library guides embedded in learning management systems (LMS) get used by students, and to identify best practices for the creation and promotion of these guides by librarians. Design – Mixed methods combining quantitative and qualitative data collection and analysis (survey, interviews, and statistical analysis). Setting – A large public university in the United States of America. Subjects – 100 undergraduate students and 14 librarians. Met...

  15. Robotic tape library system level testing at NSA: Present and planned

    Science.gov (United States)

    Shields, Michael F.

    1994-01-01

    In the present of declining Defense budgets, increased pressure has been placed on the DOD to utilize Commercial Off the Shelf (COTS) solutions to incrementally solve a wide variety of our computer processing requirements. With the rapid growth in processing power, significant expansion of high performance networking, and the increased complexity of applications data sets, the requirement for high performance, large capacity, reliable and secure, and most of all affordable robotic tape storage libraries has greatly increased. Additionally, the migration to a heterogeneous, distributed computing environment has further complicated the problem. With today's open system compute servers approaching yesterday's supercomputer capabilities, the need for affordable, reliable secure Mass Storage Systems (MSS) has taken on an ever increasing importance to our processing center's ability to satisfy operational mission requirements. To that end, NSA has established an in-house capability to acquire, test, and evaluate COTS products. Its goal is to qualify a set of COTS MSS libraries, thereby achieving a modicum of standardization for robotic tape libraries which can satisfy our low, medium, and high performance file and volume serving requirements. In addition, NSA has established relations with other Government Agencies to complete this in-house effort and to maximize our research, testing, and evaluation work. While the preponderance of the effort is focused at the high end of the storage ladder, considerable effort will be extended this year and next at the server class or mid range storage systems.

  16. Man-machine communication in Portuguese: a friendly library service system

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, H

    1982-01-01

    This paper enhances some of the main aspects of a system which converses in Portuguese to provide a library service covering the field of artificial intelligence. Its central feature is the use of logic as a single uniform language for knowledge and data representation, deductive information retrieval and linguistic analysis. The objective of designing this system was the development of a feasible method for consulting and creating data bases in natural Portuguese. The system is implemented in PROLOG, a programming language essentially identical in syntax and semantics to a subset of predicate calculus in clausal form. 32 references.

  17. Decision support system of e-book provider selection for library using Simple Additive Weighting

    Science.gov (United States)

    Ciptayani, P. I.; Dewi, K. C.

    2018-01-01

    Each library has its own criteria and differences in the importance of each criterion in choosing an e-book provider for them. The large number of providers and the different importance levels of each criterion make the problem of determining the e-book provider to be complex and take a considerable time in decision making. The aim of this study was to implement Decision support system (DSS) to assist the library in selecting the best e-book provider based on their preferences. The way of DSS works is by comparing the importance of each criterion and the condition of each alternative decision. SAW is one of DSS method that is quite simple, fast and widely used. This study used 9 criteria and 18 provider to demonstrate how SAW work in this study. With the DSS, then the decision-making time can be shortened and the calculation results can be more accurate than manual calculations.

  18. Construction and evaluation of normalized cDNA libraries enriched with full-length sequences for rapid discovery of new genes from Sisal (Agave sisalana Perr.) different developmental stages.

    Science.gov (United States)

    Zhou, Wen-Zhao; Zhang, Yan-Mei; Lu, Jun-Ying; Li, Jun-Feng

    2012-10-12

    To provide a resource of sisal-specific expressed sequence data and facilitate this powerful approach in new gene research, the preparation of normalized cDNA libraries enriched with full-length sequences is necessary. Four libraries were produced with RNA pooled from Agave sisalana multiple tissues to increase efficiency of normalization and maximize the number of independent genes by SMART™ method and the duplex-specific nuclease (DSN). This procedure kept the proportion of full-length cDNAs in the subtracted/normalized libraries and dramatically enhanced the discovery of new genes. Sequencing of 3875 cDNA clones of libraries revealed 3320 unigenes with an average insert length about 1.2 kb, indicating that the non-redundancy of libraries was about 85.7%. These unigene functions were predicted by comparing their sequences to functional domain databases and extensively annotated with Gene Ontology (GO) terms. Comparative analysis of sisal unigenes and other plant genomes revealed that four putative MADS-box genes and knotted-like homeobox (knox) gene were obtained from a total of 1162 full-length transcripts. Furthermore, real-time PCR showed that the characteristics of their transcripts mainly depended on the tight expression regulation of a number of genes during the leaf and flower development. Analysis of individual library sequence data indicated that the pooled-tissue approach was highly effective in discovering new genes and preparing libraries for efficient deep sequencing.

  19. Discover Earth: an earth system science program for libraries and their communities

    Science.gov (United States)

    Dusenbery, P.

    2011-12-01

    The view from space has deepened our understanding of Earth as a global, dynamic system. Instruments on satellites and spacecraft, coupled with advances in ground-based research, have provided us with astonishing new perspectives of our planet. Now more than ever, enhancing the public's understanding of Earth's physical and biological systems is vital to helping citizens make informed policy decisions especially when they are faced with the consequences of global climate change. While the focus for education reform is on school improvement, there is considerable research that supports the role that out-of-school experiences can play in student achievement. Libraries provide an untapped resource for engaging underserved youth and their families in fostering an appreciation and deeper understanding of science and technology topics. The Space Science Institute's National Center for Interactive Learning (NCIL) in partnership with the American Library Association (ALA), the Lunar and Planetary Institute (LPI), and the National Girls Collaborative Project (NGCP) have received funding from NSF to develop a national project called the STAR Library Education Network: a hands-on learning program for libraries and their communities (or STAR-Net for short). STAR stands for Science-Technology, Activities and Resources. STAR-Net includes two exhibitions: Discover Earth and Discover Tech. The Discover Earth exhibition will focus on local earth science topics-such as weather, water cycle, and ecosystem changes-as well as a global view of our changing planet. The main take-away message (or Big Idea) for this exhibition is that the global environment changes - and is changed by - the host community's local environment. The project team is testing whether this approach will be a good strategy for engaging the public, especially in rural America. This presentation will provide an overview of the Discover Earth project and how it is integrating climate change ideas into the exhibit

  20. Using a Gravity Model to Predict Circulation in a Public Library System.

    Science.gov (United States)

    Ottensmann, John R.

    1995-01-01

    Describes the development of a gravity model based upon principles of spatial interaction to predict the circulation of libraries in the Indianapolis-Marion County Public Library (Indiana). The model effectively predicted past circulation figures and was tested by predicting future library circulation, particularly for a new branch library.…

  1. KONTRIBUSI MODAL SOSIAL DALAM PENGEMBANGAN OTOMASI PERPUSTAKAAN: STUDI KASUS KOMUNITAS SLiMS (SENAYAN LIBRARY MANAGEMENT SYSTEM YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Yanuar Yoga Prasetyawan

    2016-11-01

    Full Text Available Today the implementation of information technology in libraries has become a basic need to organize the information, not least in Indonesia. Lack of economic capital (fund and intellectual capital (human resources that have IT educational background cause many library in Indonesia can not be able to buy or self develop the information technology. However SLiMS attend like a panacea, which capable of treating and eliminating the limitations of most libraries in Indonesia. SLiMS is an open source software for the Library Management that can be downloaded and implemented for free. In addition Slims rarely have the power owned by a software (information systems that is a SLiMS User Community. In Indonesia, SLiMS User Community has mushrooming in various regions across Indonesia, one of them in Yogyakarta. Growth and development of SLiMS User Community in Yogyakarta is a manifestation of social capital for the library with lack of information technology implementation. The purpose of this study is to reveal the values and norms are upheld by the Yogyakarta SLiMS User Community that foster social capital which drives community activities as an agent who eliminate the lack of economic capital and intellectual capital that suffered by many libraries in Indonesia. The method used is qualitative method with phenomenological approach. The results showed that the values and norms are upheld together by the Yogyakarta SLiMS User Community is becoming a agent of change of the library conditions in Indonesia, to contribute the automation development in Indonesia. That value and norms are expressed through the sinau bareng. Through sinau bareng, they were learning together to implement SLiMS. Sinau bareng was held each month by a Yogyakarta SLiMS User Community. it became the antidote the lack of libraries. So it will bring justice for all types of libraries to have the right to fulfi ll their basic needs of implementing information technology as library

  2. Featured Library: Parrish Library

    OpenAIRE

    Kirkwood, Hal P, Jr

    2015-01-01

    The Roland G. Parrish Library of Management & Economics is located within the Krannert School of Management at Purdue University. Between 2005 - 2007 work was completed on a white paper that focused on a student-centered vision for the Management & Economics Library. The next step was a massive collection reduction and a re-envisioning of both the services and space of the library. Thus began a 3 phase renovation from a 2 floor standard, collection-focused library into a single floor, 18,000s...

  3. An Integrated Nonlinear Analysis library - (INA) for solar system plasma turbulence

    Science.gov (United States)

    Munteanu, Costel; Kovacs, Peter; Echim, Marius; Koppan, Andras

    2014-05-01

    We present an integrated software library dedicated to the analysis of time series recorded in space and adapted to investigate turbulence, intermittency and multifractals. The library is written in MATLAB and provides a graphical user interface (GUI) customized for the analysis of space physics data available online like: Coordinated Data Analysis Web (CDAWeb), Automated Multi Dataset Analysis system (AMDA), Planetary Science Archive (PSA), World Data Center Kyoto (WDC), Ulysses Final Archive (UFA) and Cluster Active Archive (CAA). Three main modules are already implemented in INA : the Power Spectral Density (PSD) Analysis, the Wavelet and Intemittency Analysis and the Probability Density Functions (PDF) analysis.The layered structure of the software allows the user to easily switch between different modules/methods while retaining the same time interval for the analysis. The wavelet analysis module includes algorithms to compute and analyse the PSD, the Scalogram, the Local Intermittency Measure (LIM) or the Flatness parameter. The PDF analysis module includes algorithms for computing the PDFs for a range of scales and parameters fully customizable by the user; it also computes the Flatness parameter and enables fast comparison with standard PDF profiles like, for instance, the Gaussian PDF. The library has been already tested on Cluster and Venus Express data and we will show relevant examples. Research supported by the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no 313038/STORM, and a grant of the Romanian Ministry of National Education, CNCS UEFISCDI, project number PN-II-ID PCE-2012-4-0418.

  4. Advances in study of molecular imaging reporte gene systems

    International Nuclear Information System (INIS)

    Wu Tao; An Rui

    2010-01-01

    The use of molecular imaging reporter gene systems has allowed gene therapy to move from the laboratory to the clinical application, which provides methodology to monitor the expression of therapeutic gene noninvasively and achieve quantitative outcome in vivo. Recently, the radionuclide reporter gene still is the focus many studies, but MRI and optical reporter gene have gradually played a important part in reporter gene systems. On the basis of combination of multi-subject, for example applied chemistry and molecular biology, more and more new modified reporter genes and molecular probes have spread out. This paper mainly introduces the advantages and disadvantages of reporter gene system and development trends. (authors)

  5. The Library Work Order Processing System: A New Approach to Motivate Employees and to Increase Production in the Technical Service Department of Mercer County Community College Library. Applied Educational Research and Evaluation.

    Science.gov (United States)

    Sim, Yong Sup

    After reviewing the current movement toward job enrichment, a system was designed for the technical services department of the Mercer County Community College Library. The Library Work Order Processing System, as tried between January and March, 1974, was designed to permit each worker more variety of jobs. The technical services department was…

  6. Third-Generation Sequencing and Analysis of Four Complete Pig Liver Esterase Gene Sequences in Clones Identified by Screening BAC Library.

    Science.gov (United States)

    Zhou, Qiongqiong; Sun, Wenjuan; Liu, Xiyan; Wang, Xiliang; Xiao, Yuncai; Bi, Dingren; Yin, Jingdong; Shi, Deshi

    2016-01-01

    Pig liver carboxylesterase (PLE) gene sequences in GenBank are incomplete, which has led to difficulties in studying the genetic structure and regulation mechanisms of gene expression of PLE family genes. The aim of this study was to obtain and analysis of complete gene sequences of PLE family by screening from a Rongchang pig BAC library and third-generation PacBio gene sequencing. After a number of existing incomplete PLE isoform gene sequences were analysed, primers were designed based on conserved regions in PLE exons, and the whole pig genome used as a template for Polymerase chain reaction (PCR) amplification. Specific primers were then selected based on the PCR amplification results. A three-step PCR screening method was used to identify PLE-positive clones by screening a Rongchang pig BAC library and PacBio third-generation sequencing was performed. BLAST comparisons and other bioinformatics methods were applied for sequence analysis. Five PLE-positive BAC clones, designated BAC-10, BAC-70, BAC-75, BAC-119 and BAC-206, were identified. Sequence analysis yielded the complete sequences of four PLE genes, PLE1, PLE-B9, PLE-C4, and PLE-G2. Complete PLE gene sequences were defined as those containing regulatory sequences, exons, and introns. It was found that, not only did the PLE exon sequences of the four genes show a high degree of homology, but also that the intron sequences were highly similar. Additionally, the regulatory region of the genes contained two 720bps reverse complement sequences that may have an important function in the regulation of PLE gene expression. This is the first report to confirm the complete sequences of four PLE genes. In addition, the study demonstrates that each PLE isoform is encoded by a single gene and that the various genes exhibit a high degree of sequence homology, suggesting that the PLE family evolved from a single ancestral gene. Obtaining the complete sequences of these PLE genes provides the necessary foundation for

  7. The library

    International Nuclear Information System (INIS)

    1980-01-01

    A specialized library is essential for conducting the research work of the Uranium Institute. The need was recognized at the foundation of the Institute and a full-time librarian was employed in 1976 to establish the necessary systems and begin the task of building up the collection. A brief description is given of the services offered by the library which now contains books, periodicals, pamphlets and press cuttings, focussed on uranium and nuclear energy, but embracing economics, politics, trade, legislation, geology, mining and mineral processing, environmental protection and nuclear technology. (author)

  8. IAEA technical meeting on nuclear data library for advanced systems - Fusion devices

    International Nuclear Information System (INIS)

    Forrest, R.; Mengoni, A.

    2008-04-01

    A Technical Meeting on 'Nuclear Data Library for Advanced Systems - Fusion Devices' was held at the IAEA Headquarters in Vienna from 31 October to 2 November 2007. The main objective of the initiative has been to define a proposal and detailed plan of activities for a Co-ordinated Research Project on this subject. Details of the discussions which took place at the meeting, including a review of the current activities in the field, a list of recommendations and a proposed timeline schedule for the CRP are summarized in this report. (author)

  9. Generation of dTALEs and Libraries of Synthetic TALE-Activated Promoters for Engineering of Gene Regulatory Networks in Plants.

    Science.gov (United States)

    Schreiber, Tom; Tissier, Alain

    2017-01-01

    Transcription factors with programmable DNA-binding specificity constitute valuable tools for the design of orthogonal gene regulatory networks for synthetic biology. Transcription activator-like effectors (TALEs), as natural transcription regulators, were used to design, build, and test libraries of synthetic TALE-activated promoters (STAPs) that show a broad range of expression levels in plants. In this chapter, we present protocols for the construction of artificial TALEs and corresponding STAPs.

  10. Nuclear computerized library for assessing reactor reliability (NUCLARR): User's guide: Part 3, NUCLARR system description

    International Nuclear Information System (INIS)

    Gilmore, W.E.; Gentillon, C.D.; Gertman, D.I.; Beers, G.H.; Galyean, W.J.; Gilbert, B.G.

    1988-06-01

    The Nuclear Computerized Library for Assessing Reactor Reliability (NUCLARR) is an automated data base management system for processing and storing human error probability and hardware component failure data. The NUCLARR system software resides on an IBM (or compatible) personal micro-computer. NUCLARR can be used by the end user to furnish data inputs for both human and hardware reliability analysis in support of a variety of risk assessment activities. The NUCLARR system is documented in a five-volume series of reports. Volume IV of this series is the User's Guide for operating the NUCLARR software and is presented in three parts. This document, Part 3: NUCLARR System Description, provides an in-depth discussion of the design characteristics and special features of the NUCLARR software. Part 3 also presents the organization of the data base structures and techniques used to manipulate the data

  11. New genes of Xanthomonas citri subsp. citri involved in pathogenesis and adaptation revealed by a transposon-based mutant library.

    Science.gov (United States)

    Laia, Marcelo L; Moreira, Leandro M; Dezajacomo, Juliana; Brigati, Joice B; Ferreira, Cristiano B; Ferro, Maria I T; Silva, Ana C R; Ferro, Jesus A; Oliveira, Julio C F

    2009-01-16

    Citrus canker is a disease caused by the phytopathogens Xanthomonas citri subsp. citri, Xanthomonas fuscans subsp. aurantifolli and Xanthomonas alfalfae subsp. citrumelonis. The first of the three species, which causes citrus bacterial canker type A, is the most widely spread and severe, attacking all citrus species. In Brazil, this species is the most important, being found in practically all areas where citrus canker has been detected. Like most phytobacterioses, there is no efficient way to control citrus canker. Considering the importance of the disease worldwide, investigation is needed to accurately detect which genes are related to the pathogen-host adaptation process and which are associated with pathogenesis. Through transposon insertion mutagenesis, 10,000 mutants of Xanthomonas citri subsp. citri strain 306 (Xcc) were obtained, and 3,300 were inoculated in Rangpur lime (Citrus limonia) leaves. Their ability to cause citrus canker was analyzed every 3 days until 21 days after inoculation; a set of 44 mutants showed altered virulence, with 8 presenting a complete loss of causing citrus canker symptoms. Sequencing of the insertion site in all 44 mutants revealed that 35 different ORFs were hit, since some ORFs were hit in more than one mutant, with mutants for the same ORF presenting the same phenotype. An analysis of these ORFs showed that some encoded genes were previously known as related to pathogenicity in phytobacteria and, more interestingly, revealed new genes never implicated with Xanthomonas pathogenicity before, including hypothetical ORFs. Among the 8 mutants with no canker symptoms are the hrpB4 and hrpX genes, two genes that belong to type III secretion system (TTSS), two hypothetical ORFS and, surprisingly, the htrA gene, a gene reported as involved with the virulence process in animal-pathogenic bacteria but not described as involved in phytobacteria virulence. Nucleic acid hybridization using labeled cDNA probes showed that some of the

  12. Study on interface between nuclear material accounting system and national nuclear forensic library

    International Nuclear Information System (INIS)

    Jeong, Yonhong; Han, Jae-Jun; Chang, Sunyoung; Shim, Hye-Won; Ahn, Seungho

    2016-01-01

    The implementation of nuclear forensics requires physical, chemical and radiological characteristics with transport history to unravel properties of seized nuclear materials. For timely assessment provided in the ITWG guideline, development of national response system (e.g., national nuclear forensic library) is strongly recommended. Nuclear material accounting is essential to obtain basic data in the nuclear forensic implementation phase from the perspective of nuclear non-proliferation related to the IAEA Safeguards and nuclear security. In this study, the nuclear material accounting reports were chosen due to its well-established procedure, and reviewed how to efficiently utilize the existing material accounting system to the nuclear forensic implementation phase In conclusion, limits and improvements in implementing the nuclear forensics were discussed. This study reviewed how to utilize the existing material accounting system for implementing nuclear forensics. Concerning item counting facility, nuclear material properties can be obtained based on nuclear material accounting information. Nuclear fuel assembly data being reported for the IAEA Safeguards can be utilized as unique identifier within the back-end fuel cycle. Depending upon the compulsory accountability report period, there exist time gaps. If national capabilities ensure that history information within the front-end nuclear fuel cycle is traceable particularly for the bulk handling facility, the entire cycle of national nuclear fuel would be managed in the framework of developing a national nuclear forensic library

  13. Study on interface between nuclear material accounting system and national nuclear forensic library

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Yonhong; Han, Jae-Jun; Chang, Sunyoung; Shim, Hye-Won; Ahn, Seungho [Korea Institute of Nuclear Non-proliferation and Control, Daejeon (Korea, Republic of)

    2016-10-15

    The implementation of nuclear forensics requires physical, chemical and radiological characteristics with transport history to unravel properties of seized nuclear materials. For timely assessment provided in the ITWG guideline, development of national response system (e.g., national nuclear forensic library) is strongly recommended. Nuclear material accounting is essential to obtain basic data in the nuclear forensic implementation phase from the perspective of nuclear non-proliferation related to the IAEA Safeguards and nuclear security. In this study, the nuclear material accounting reports were chosen due to its well-established procedure, and reviewed how to efficiently utilize the existing material accounting system to the nuclear forensic implementation phase In conclusion, limits and improvements in implementing the nuclear forensics were discussed. This study reviewed how to utilize the existing material accounting system for implementing nuclear forensics. Concerning item counting facility, nuclear material properties can be obtained based on nuclear material accounting information. Nuclear fuel assembly data being reported for the IAEA Safeguards can be utilized as unique identifier within the back-end fuel cycle. Depending upon the compulsory accountability report period, there exist time gaps. If national capabilities ensure that history information within the front-end nuclear fuel cycle is traceable particularly for the bulk handling facility, the entire cycle of national nuclear fuel would be managed in the framework of developing a national nuclear forensic library.

  14. A Security Mechanism for library management system using low cost RFID tags

    Directory of Open Access Journals (Sweden)

    V. Nagalakshmi

    2007-02-01

    Full Text Available Radio Frequency Identification (RFID systems will become pervasive in our daily lives due to their low cost and easy to use characteristics[1]. This paper presents a methodology of using low cost RFID tags for a library management system to protect the books from unauthorized capturing and usage. Every object to be identified in the RFID system is physically labeled with a tag. In the proposed method a book or a magazine or a CD is identified with the RFID tag. Whenever a book is issued to any user of the library, the RFID reader will capture the information of that book and compares it with the related information of the book in the Online Public Access Catalogue (OPAC and issues the book to the user depending on his identity in the database. If any unauthorized person intends to take the book the RFID reader will immediately respond to that and make sure that it was not issued. If any body tries to remove the tag it can be traced with the help of location device. Keywords: RFID, OPAC, Unauthorized user, tag, Reader.

  15. Nuclear Data Libraries for Advanced Systems - Fusion Devices (FENDL 3.0). Summary report of the Third Research Coordination Meeting

    International Nuclear Information System (INIS)

    Sawan, Mohamed E.

    2012-03-01

    The third Research Co-ordination Meeting of the Nuclear Data Libraries for Advanced Systems - Fusion Devices (FENDL-3) was held at IAEA Headquarters in Vienna from 6 to 9 December 2011. A summary of the presentations given during meeting is given in this report along with the discussions that took place. A list of actions necessary to complete the library production, processing and testing are given. Details of the documents arising from the CRP were agreed. (author)

  16. Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1.

    Science.gov (United States)

    Park, Jeongbin; Bae, Sangsu

    2018-03-15

    Following the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of optimally selecting guide RNAs (gRNAs) among all possible genome-wide target sites. Here, we present Cpf1-Database, a genome-wide gRNA library design tool for LbCpf1 and AsCpf1, which have DNA recognition sequences of 5'-TTTN-3' at the 5' ends of target sites. Cpf1-Database provides a sophisticated but simple way to design gRNAs for AsCpf1 nucleases on the genome scale. One can easily access the data using a straightforward web interface, and using the powerful collections feature one can easily design gRNAs for thousands of genes in short time. Free access at http://www.rgenome.net/cpf1-database/. sangsubae@hanyang.ac.kr.

  17. Charting a Course through CORAL: Texas A&M University Libraries' Experience Implementing an Open-Source Electronic Resources Management System

    Science.gov (United States)

    Hartnett, Eric; Beh, Eugenia; Resnick, Taryn; Ugaz, Ana; Tabacaru, Simona

    2013-01-01

    In 2010, after two previous unsuccessful attempts at electronic resources management system (ERMS) implementation, Texas A&M University (TAMU) Libraries set out once again to find an ERMS that would fit its needs. After surveying the field, TAMU Libraries selected the University of Notre Dame Hesburgh Libraries-developed, open-source ERMS,…

  18. Recent Trends of Polymer Mediated Liposomal Gene Delivery System

    Directory of Open Access Journals (Sweden)

    Shyamal Kumar Kundu

    2014-01-01

    Full Text Available Advancement in the gene delivery system have resulted in clinical successes in gene therapy for patients with several genetic diseases, such as immunodeficiency diseases, X-linked adrenoleukodystrophy (X-ALD blindness, thalassemia, and many more. Among various delivery systems, liposomal mediated gene delivery route is offering great promises for gene therapy. This review is an attempt to depict a portrait about the polymer based liposomal gene delivery systems and their future applications. Herein, we have discussed in detail the characteristics of liposome, importance of polymer for liposome formulation, gene delivery, and future direction of liposome based gene delivery as a whole.

  19. School Libraries and Innovation

    Science.gov (United States)

    McGrath, Kevin G.

    2015-01-01

    School library programs have measured success by improved test scores. But how do next-generation school libraries demonstrate success as they strive to be centers of innovation and creativity? These libraries offer solutions for school leaders who struggle to restructure existing systems built around traditional silos of learning (subjects and…

  20. Development of an Integrated, Computer-Based Bibliographical Data System for a Large University Library. Annual Report to the National Science Foundation from the University of Chicago Library, 1966/67.

    Science.gov (United States)

    Fussler, Herman; Payne, Charles T.

    Part I is a discussion of the following project tasks: A) development of an on-line, real-time bibliographic data processing system; B) implementation in library operations; C) character sets; D) Project MARC; E) circulation; and F) processing operation studies. Part II is a brief discussion of efforts to work out cooperative library systems…

  1. DESIGNING OF ELECTRONIC LIBRARIES SYSTEM OF SCIENTIFIC AND EDUCATIONAL INSTITUTIONS OF APS OF UKRAINE

    Directory of Open Access Journals (Sweden)

    Oleg M. Spirin

    2010-08-01

    Full Text Available In the article the concept of electronic library is considered, preconditions of its designing for the institutions of APS of Ukraine are investigated. The basic components, base services of electronic library and kinds of corresponding resources are defined. It is proved typical decisions for working out of electronic scientifically-educational library of APS of Ukraine.

  2. The Personnel, Material and Financial Resources of the Library Systems of 311 Industrial Corporations.

    Science.gov (United States)

    Jackson, Eugene B.; Jackson, Ruth L.

    An indepth study of the FORTUNE 500 Corporations' libraries has revealed consistent characteristics across 27 industrial classifications, some of which parallel the divisions of professional associations, such as the Special Libraries Association. Partial profiles of Pharmaceutical, Aircraft, and Food Libraries are given as examples, with the…

  3. The alternative library

    OpenAIRE

    Collinson, Timothy; Williams, A.

    2004-01-01

    Much time and effort has been devoted to designing and developing library Web sites that are easy to navigate by both new students and experienced researchers. In a review of the Southampton Institute Library it was decided that in addition to updating the existing homepage an alternative would be offered. Drawing on theory relating to user interface design, learning styles and creative thinking, an Alternative Library navigation system was added to the more traditional library homepage. The ...

  4. FENDL multigroup libraries

    International Nuclear Information System (INIS)

    Ganesan, S.; Muir, D.W.

    1992-01-01

    Selected neutron reaction nuclear data libraries and photon-atomic interaction cross section libraries for elements of interest to the IAEA's program on Fusion Evaluated Nuclear Data Library (FENDL) have been processed into MATXSR format using the NJOY system on the VAX4000 computer of the IAEA. This document lists the resulting multigroup data libraries. All the multigroup data generated are available cost-free upon request from the IAEA Nuclear Data Section. (author). 9 refs

  5. Construction of a cDNA library from female adult of Toxocara canis, and analysis of EST and immune-related genes expressions.

    Science.gov (United States)

    Zhou, Rongqiong; Xia, Qingyou; Huang, Hancheng; Lai, Min; Wang, Zhenxin

    2011-10-01

    Toxocara canis is a widespread intestinal nematode parasite of dogs, which can also cause disease in humans. We employed an expressed sequence tag (EST) strategy in order to study gene-expression including development, digestion and reproduction of T. canis. ESTs provided a rapid way to identify genes, particularly in organisms for which we have very little molecular information. In this study, a cDNA library was constructed from a female adult of T. canis and 215 high-quality ESTs from 5'-ends of the cDNA clones representing 79 unigenes were obtained. The titer of the primary cDNA library was 1.83×10(6)pfu/mL with a recombination rate of 99.33%. Most of the sequences ranged from 300 to 900bp with an average length of 656bp. Cluster analysis of these ESTs allowed identification of 79 unique sequences containing 28 contigs and 51 singletons. BLASTX searches revealed that 18 unigenes (22.78% of the total) or 70 ESTs (32.56% of the total) were novel genes that had no significant matches to any protein sequences in the public databases. The rest of the 61 unigenes (77.22% of the total) or 145 ESTs (67.44% of the total) were closely matched to the known genes or sequences deposited in the public databases. These genes were classified into seven groups based on their known or putative biological functions. We also confirmed the gene expression patterns of several immune-related genes using RT-PCR examination. This work will provide a valuable resource for the further investigations in the stage-, sex- and tissue-specific gene transcription or expression. Copyright © 2011. Published by Elsevier Inc.

  6. PsiQuaSP-A library for efficient computation of symmetric open quantum systems.

    Science.gov (United States)

    Gegg, Michael; Richter, Marten

    2017-11-24

    In a recent publication we showed that permutation symmetry reduces the numerical complexity of Lindblad quantum master equations for identical multi-level systems from exponential to polynomial scaling. This is important for open system dynamics including realistic system bath interactions and dephasing in, for instance, the Dicke model, multi-Λ system setups etc. Here we present an object-oriented C++ library that allows to setup and solve arbitrary quantum optical Lindblad master equations, especially those that are permutationally symmetric in the multi-level systems. PsiQuaSP (Permutation symmetry for identical Quantum Systems Package) uses the PETSc package for sparse linear algebra methods and differential equations as basis. The aim of PsiQuaSP is to provide flexible, storage efficient and scalable code while being as user friendly as possible. It is easily applied to many quantum optical or quantum information systems with more than one multi-level system. We first review the basics of the permutation symmetry for multi-level systems in quantum master equations. The application of PsiQuaSP to quantum dynamical problems is illustrated with several typical, simple examples of open quantum optical systems.

  7. Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440

    Energy Technology Data Exchange (ETDEWEB)

    Elmore, Joshua R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Furches, Anna [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Wolff, Gara N. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Gorday, Kent [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; Guss, Adam M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division

    2017-04-15

    Pseudomonas putida strains are highly robust bacteria known for their ability to efficiently utilize a variety of carbon sources, including aliphatic and aromatic hydrocarbons. Recently, P. putida has been engineered to valorize the lignin stream of a lignocellulosic biomass pretreatment process. Nonetheless, when compared to platform organisms such as Escherichia coli, the toolkit for engineering P. putida is underdeveloped. Heterologous gene expression in particular is problematic. Plasmid instability and copy number variance provide challenges for replicative plasmids, while use of homologous recombination for insertion of DNA into the chromosome is slow and laborious. Furthermore, heterologous expression efforts to date typically rely on overexpression of exogenous pathways using a handful of poorly characterized promoters. In order to improve the P. putida toolkit, we developed a rapid genome integration system using the site-specific recombinase from bacteriophage Bxb1 to enable rapid, high efficiency integration of DNA into the P. putida chromosome. We also developed a library of synthetic promoters with various UP elements, -35 sequences, and -10 sequences, as well as different ribosomal binding sites. We tested these promoters using a fluorescent reporter gene, mNeonGreen, to characterize the strength of each promoter, and identified UP-element-promoter-ribosomal binding sites combinations capable of driving a ~150-fold range of protein expression levels. One additional integrating vector was developed that confers more robust kanamycin resistance when integrated at single copy into the chromosome. This genome integration and reporter systems are extensible for testing other genetic parts, such as examining terminator strength, and will allow rapid integration of heterologous pathways for metabolic engineering.

  8. Library/vendor relationships

    CERN Document Server

    Brooks, Sam

    2014-01-01

    A view of the mutual dependence between libraries and vendorsAs technology advances, libraries are forced to reach beyond their own resources to find effective ways to maintain accuracy and superior service levels. Vendors provide databases and integrated library systems that perform those functions for profit. Library/Vendor Relationships examines the increasing cooperation in which libraries find they must participate in, and vice versa, with the vendors that provide system infrastructure and software. Expert contributors provide insights from all sides of this unique collaboration, offering

  9. Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds

    Directory of Open Access Journals (Sweden)

    Sugantham Priyanka Annabel

    2010-10-01

    Full Text Available Abstract Background Jatropha curcas L. is promoted as an important non-edible biodiesel crop worldwide. Jatropha oil, which is a triacylglycerol, can be directly blended with petro-diesel or transesterified with methanol and used as biodiesel. Genetic improvement in jatropha is needed to increase the seed yield, oil content, drought and pest resistance, and to modify oil composition so that it becomes a technically and economically preferred source for biodiesel production. However, genetic improvement efforts in jatropha could not take advantage of genetic engineering methods due to lack of cloned genes from this species. To overcome this hurdle, the current gene discovery project was initiated with an objective of isolating as many functional genes as possible from J. curcas by large scale sequencing of expressed sequence tags (ESTs. Results A normalized and full-length enriched cDNA library was constructed from developing seeds of J. curcas. The cDNA library contained about 1 × 106 clones and average insert size of the clones was 2.1 kb. Totally 12,084 ESTs were sequenced to average high quality read length of 576 bp. Contig analysis revealed 2258 contigs and 4751 singletons. Contig size ranged from 2-23 and there were 7333 ESTs in the contigs. This resulted in 7009 unigenes which were annotated by BLASTX. It showed 3982 unigenes with significant similarity to known genes and 2836 unigenes with significant similarity to genes of unknown, hypothetical and putative proteins. The remaining 191 unigenes which did not show similarity with any genes in the public database may encode for unique genes. Functional classification revealed unigenes related to broad range of cellular, molecular and biological functions. Among the 7009 unigenes, 6233 unigenes were identified to be potential full-length genes. Conclusions The high quality normalized cDNA library was constructed from developing seeds of J. curcas for the first time and 7009 unigenes coding

  10. Identification of an extensive gene cluster among a family of PPOs in Trifolium pratense L. (red clover using a large insert BAC library

    Directory of Open Access Journals (Sweden)

    Thomas Ann

    2009-07-01

    Full Text Available Abstract Background Polyphenol oxidase (PPO activity in plants is a trait with potential economic, agricultural and environmental impact. In relation to the food industry, PPO-induced browning causes unacceptable discolouration in fruit and vegetables: from an agriculture perspective, PPO can protect plants against pathogens and environmental stress, improve ruminant growth by increasing nitrogen absorption and decreasing nitrogen loss to the environment through the animal's urine. The high PPO legume, red clover, has a significant economic and environmental role in sustaining low-input organic and conventional farms. Molecular markers for a range of important agricultural traits are being developed for red clover and improved knowledge of PPO genes and their structure will facilitate molecular breeding. Results A bacterial artificial chromosome (BAC library comprising 26,016 BAC clones with an average 135 Kb insert size, was constructed from Trifolium pratense L. (red clover, a diploid legume with a haploid genome size of 440–637 Mb. Library coverage of 6–8 genome equivalents ensured good representation of genes: the library was screened for polyphenol oxidase (PPO genes. Two single copy PPO genes, PPO4 and PPO5, were identified to add to a family of three, previously reported, paralogous genes (PPO1–PPO3. Multiple PPO1 copies were identified and characterised revealing a subfamily comprising three variants PPO1/2, PPO1/4 and PPO1/5. Six PPO genes clustered within the genome: four separate BAC clones could be assembled onto a predicted 190–510 Kb single BAC contig. Conclusion A PPO gene family in red clover resides as a cluster of at least 6 genes. Three of these genes have high homology, suggesting a more recent evolutionary event. This PPO cluster covers a longer region of the genome than clusters detected in rice or previously reported in tomato. Full-length coding sequences from PPO4, PPO5, PPO1/5 and PPO1/4 will facilitate

  11. Construction of a 7-fold BAC library and cytogenetic mapping of 10 genes in the giant panda (Ailuropoda melanoleuca)

    OpenAIRE

    Liu, Wei; Zhao, Yonghui; Liu, Zhaoliang; Zhang, Ying; Lian, Zhengxing; Li, Ning

    2006-01-01

    Abstract Background The giant panda, one of the most primitive carnivores, is an endangered animal. Although it has been the subject of many interesting studies during recent years, little is known about its genome. In order to promote research on this genome, a bacterial artificial chromosome (BAC) library of the giant panda was constructed in this study. Results This BAC library contains 198,844 clones with an average insert size of 108 kb, which represents approximately seven equivalents o...

  12. Facile synthesis of semi-library of low charge density cationic polyesters from poly(alkylene maleate)s for efficient local gene delivery.

    Science.gov (United States)

    Yan, Huijie; Zhu, Dingcheng; Zhou, Zhuxian; Liu, Xin; Piao, Ying; Zhang, Zhen; Liu, Xiangrui; Tang, Jianbin; Shen, Youqing

    2018-03-30

    Cationic polymers are one of the main non-viral vectors for gene therapy, but their applications are hindered by the toxicity and inefficient transfection, particularly in the presence of serum or other biological fluids. While rational design based on the current understanding of gene delivery process has produced various cationic polymers with improved overall transfection, high-throughput parallel synthesis of libraries of cationic polymers seems a more effective strategy to screen out efficacious polymers. Herein, we demonstrate a novel platform for parallel synthesis of low cationic charge-density polyesters for efficient gene delivery. Unsaturated polyester poly(alkylene maleate) (PAM) readily underwent Michael-addition reactions with various mercaptamines to produce polyester backbones with pendant amine groups, poly(alkylene maleate mercaptamine)s (PAMAs). Variations of the alkylenes in the backbone and the mercaptamines on the side chain produced PAMAs with tunable hydrophobicity and DNA-condensation ability, the key parameters dominating transfection efficiency of the resulting polymer/DNA complexes (polyplexes). A semi-library of such PAMAs was exampled from 7 alkylenes and 18 mercaptamines, from which a lead PAMA, G-1, synthesized from poly(1,4-phenylene bis(methylene) maleate) and N,N-dimethylcysteamine, showed remarkable transfection efficiency even in the presence of serum, owing to its efficient lysosome-circumventing cellular uptake. Furthermore, G-1 polyplexes efficiently delivered the suicide gene pTRAIL to intraperitoneal tumors and elicited effective anticancer activity. Copyright © 2018 Elsevier Ltd. All rights reserved.

  13. Construction of an adult barnacle (Balanus amphitrite cDNA library and selection of reference genes for quantitative RT-PCR studies

    Directory of Open Access Journals (Sweden)

    Burgess J Grant

    2009-06-01

    Full Text Available Abstract Background Balanus amphitrite is a barnacle commonly used in biofouling research. Although many aspects of its biology have been elucidated, the lack of genetic information is impeding a molecular understanding of its life cycle. As part of a wider multidisciplinary approach to reveal the biogenic cues influencing barnacle settlement and metamorphosis, we have sequenced and annotated the first cDNA library for B. amphitrite. We also present a systematic validation of potential reference genes for normalization of quantitative real-time PCR (qRT-PCR data obtained from different developmental stages of this animal. Results We generated a cDNA library containing expressed sequence tags (ESTs from adult B. amphitrite. A total of 609 unique sequences (comprising 79 assembled clusters and 530 singlets were derived from 905 reliable unidirectionally sequenced ESTs. Bioinformatics tools such as BLAST, HMMer and InterPro were employed to allow functional annotation of the ESTs. Based on these analyses, we selected 11 genes to study their ability to normalize qRT-PCR data. Total RNA extracted from 7 developmental stages was reverse transcribed and the expression stability of the selected genes was compared using geNorm, BestKeeper and NormFinder. These software programs produced highly comparable results, with the most stable gene being mt-cyb, while tuba, tubb and cp1 were clearly unsuitable for data normalization. Conclusion The collection of B. amphitrite ESTs and their annotation has been made publically available representing an important resource for both basic and applied research on this species. We developed a qRT-PCR assay to determine the most reliable reference genes. Transcripts encoding cytochrome b and NADH dehydrogenase subunit 1 were expressed most stably, although other genes also performed well and could prove useful to normalize gene expression studies.

  14. Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

    Directory of Open Access Journals (Sweden)

    Keisuke Fukunaga

    2012-01-01

    Full Text Available Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available premade phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This paper focuses on what should be born in mind for beginners using commercially available cloning kits (Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively. In the M13 system, Pro or a basic amino acid (especially, Arg should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.

  15. Research Library

    Science.gov (United States)

    Los Alamos National Laboratory Research Library Search Site submit Contact Us | Remote Access Standards Theses/Dissertations Research Help Subject Guides Library Training Video Tutorials Alerts Research Library: delivering essential knowledge services for national security sciences since 1947 Los

  16. Designing an Academic Library as a Place and a Space: How a Robotic Storage System Will Create a 21st Century Library Design

    Science.gov (United States)

    Bostick, Sharon L.; Irwin, Bryan

    2012-01-01

    Renovating, expanding or building new libraries today is a challenge on several levels. Libraries in general are faced with image issues, such as the assumption that a library building exists only to house print material followed by the equally erroneous assumption that everything is now available online, thus rendering a physical building…

  17. Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths: A Useful Resource for Comparative Genomics of Lepidoptera

    Directory of Open Access Journals (Sweden)

    Yuji Yasukochi

    2011-01-01

    Full Text Available Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, =31, which are not closely related with each other or with the silkworm, Bombyx mori, (=28, the sequenced model lepidopteran. A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH, as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

  18. Public libraries in the library regions in the year 2009

    Directory of Open Access Journals (Sweden)

    Milena Bon

    2011-01-01

    Full Text Available Purpose: Regional public libraries were initiated in 2003 to connect professional activities of libraries within regional networks and to ensure coordinated library development in a region in cooperation with the Library System Development Centre at the National and University Library performing a coordinating role. The article analyses the performance of public libraries and their integration in regional library networks in order to find out the level of development of conditions of performance of public libraries.Methodology/approach: Statistical data for the year 2009 were the basis for the overview of library activities of ten library regions with regard to applicable legislation and library standards. The level of regional library activities is compared to the socio-economic situation of statistical regions thus representing a new approach to the presentation of Slovenian’s public libraries’ development.Results: Absolute values indicate better development of nine libraries in the central Slovenia region while relative values offer a totally different picture. Four libraries in the region of Nova Gorica prove the highest level of development.Research limitation: Research is limited to the year 2009 and basic statistical analysis.Originality/practical implications: Findings of the analysis are useful for public libraries to plan their development strategy within a region and for financial bodies to provide for adequate financing for library activities in a specific region. The basic condition for successful public library performance is the even and harmonized development of conditions of performance as recommended by library standards.

  19. Providing Health Sciences Services in a Joint-Use Distributed Learning Library System: An Organizational Case Study.

    Science.gov (United States)

    Enslow, Electra; Fricke, Suzanne; Vela, Kathryn

    2017-01-01

    The purpose of this organizational case study is to describe the complexities librarians face when serving a multi-campus institution that supports both a joint-use library and expanding health sciences academic partnerships. In a system without a centralized health science library administration, liaison librarians are identifying dispersed programs and user groups and collaborating to define their unique service and outreach needs within a larger land-grant university. Using a team-based approach, health sciences librarians are communicating to integrate research and teaching support, systems differences across dispersed campuses, and future needs of a new community-based medical program.

  20. The Digital Library for Earth System Education (DLESE): Progress and Opportunities for Engagement

    Science.gov (United States)

    Marlino, M. R.; Pandya, R. E.

    2003-12-01

    The Digital Library for Earth System Education (DLESE) is a community-led, NSF-funded effort to promote access to high-quality digital resources for teaching and learning about the Earth. Now in its fourth year of development, DLESE is used by tens of thousands of educators and learners a month, and provides access to over 5000 educational resources. These resources include a variety of media formats, from text-based lesson plans to sophisticated tools for interactive three-dimensional visualization of authentic scientific data. In August of 2003, DLESE introduced Version 2.0 of the library. The new version features enhanced educational services including: the ability to search by National Science and Geography Standards, the ability to search over multiple, discrete collections, and annotation services that allow educators to evaluate resources and supply tips for their effective use. With version 2.0, DLESE can better provide services to support researchers through the entire cycle of their educational and outreach efforts. These services are of particular value to researchers when developing, disseminating, and evaluating educational materials in their efforts to meet the National Science Foundation's Criteria Two, for proposals. To support development efforts, we have published best-practices guides for developing resources; we actively broker relationships between the scientific community, instructional designers, and educational researchers; and we are leading efforts to integrate data into educational materials. The DLESE Evaluation Services offers broad support to a variety of research efforts. The DLESE Program Center provides technology integration and operational systems to support partnerships with the research community.

  1. A Constitution for Danish Libraries.

    Science.gov (United States)

    Nielsen, O. Perch

    This overview of the history of legislation governing the Danish library system from 1920 to the present: describes the various kinds of libraries in Denmark, explores the current controversies surrounding the roles of several supervisory library bodies, and details recent recommendations of the Danish Library Commission. (FM)

  2. Nonviral Delivery Systems For Cancer Gene Therapy: Strategies And Challenges.

    Science.gov (United States)

    Shim, Gayong; Kim, Dongyoon; Le, Quoc-Viet; Park, Gyu Thae; Kwon, Taekhyun; Oh, Yu-Kyoung

    2018-01-19

    Gene therapy has been receiving widespread attention due to its unique advantage in regulating the expression of specific target genes. In the field of cancer gene therapy, modulation of gene expression has been shown to decrease oncogenic factors in cancer cells or increase immune responses against cancer. Due to the macromolecular size and highly negative physicochemical features of plasmid DNA, efficient delivery systems are an essential ingredient for successful gene therapy. To date, a variety of nanostructures and materials have been studied as nonviral gene delivery systems. In this review, we will cover nonviral delivery strategies for cancer gene therapy, with a focus on target cancer genes and delivery materials. Moreover, we will address current challenges and perspectives for nonviral delivery-based cancer gene therapeutics. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  3. Library Classification 2020

    Science.gov (United States)

    Harris, Christopher

    2013-01-01

    In this article the author explores how a new library classification system might be designed using some aspects of the Dewey Decimal Classification (DDC) and ideas from other systems to create something that works for school libraries in the year 2020. By examining what works well with the Dewey Decimal System, what features should be carried…

  4. A transposon mutant library of Bacillus cereus ATCC 10987 reveals novel genes required for biofilm formation and implicates motility as an important factor for pellicle-biofilm formation.

    Science.gov (United States)

    Okshevsky, Mira; Louw, Matilde Greve; Lamela, Elena Otero; Nilsson, Martin; Tolker-Nielsen, Tim; Meyer, Rikke Louise

    2018-04-01

    Bacillus cereus is one of the most common opportunistic pathogens causing foodborne illness, as well as a common source of contamination in the dairy industry. B. cereus can form robust biofilms on food processing surfaces, resulting in food contamination due to shedding of cells and spores. Despite the medical and industrial relevance of this species, the genetic basis of biofilm formation in B. cereus is not well studied. In order to identify genes required for biofilm formation in this bacterium, we created a library of 5000 +  transposon mutants of the biofilm-forming strain B. cereusATCC 10987, using an unbiased mariner transposon approach. The mutant library was screened for the ability to form a pellicle biofilm at the air-media interface, as well as a submerged biofilm at the solid-media interface. A total of 91 genes were identified as essential for biofilm formation. These genes encode functions such as chemotaxis, amino acid metabolism and cellular repair mechanisms, and include numerous genes not previously known to be required for biofilm formation. Although the majority of disrupted genes are not directly responsible for motility, further investigations revealed that the vast majority of the biofilm-deficient mutants were also motility impaired. This observation implicates motility as a pivotal factor in the formation of a biofilm by B. cereus. These results expand our knowledge of the fundamental molecular mechanisms of biofilm formation by B. cereus. © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  5. Optogenetic dissection of neuronal circuits in zebrafish using viral gene transfer and the Tet system

    Directory of Open Access Journals (Sweden)

    Peixin Zhu

    2009-12-01

    Full Text Available The conditional expression of transgenes at high levels in sparse and specific populations of neurons is important for high-resolution optogenetic analyses of neuronal circuits. We explored two complementary methods, viral gene delivery and the iTet-Off system, to express transgenes in the brain of zebrafish. High-level gene expression in neurons was achieved by Sindbis and Rabies viruses. The Tet system produced strong and specific gene expression that could be modulated conveniently by doxycycline. Moreover, transgenic lines showed expression in distinct, sparse and stable populations of neurons that appeared to be subsets of the neurons targeted by the promoter driving the Tet activator. The Tet system therefore provides the opportunity to generate libraries of diverse expression patterns similar to gene trap approaches or the thy-1 promoter in mice, but with the additional possibility to pre-select cell types of interest. In transgenic lines expressing channelrhodopsin-2, action potential firing could be precisely controlled by two-photon stimulation at low laser power, presumably because the expression levels of the Tet-controlled genes were high even in adults. In channelrhodopsin-2-expressing larvae, optical stimulation with a single blue LED evoked distinct swimming behaviors including backward swimming. These approaches provide new opportunities for the optogenetic dissection of neuronal circuit structure and function.

  6. Software for the computized neutron data library of the SOKRATOR system

    International Nuclear Information System (INIS)

    Kolesov, V.E.; Krivtsov, A.S.; Solov'ev, N.A.

    1976-01-01

    When preparing data for nuclear reactors and shield computations using the evaluated nuclear data library it is necessary to have a set of special service programs to maintain the library itself. In this paper the structure of this set is discussed and a brief description of some programs is presented

  7. Special Features of the Advanced Loans Module of the ABCD Integrated Library System

    Science.gov (United States)

    de Smet, Egbert

    2011-01-01

    Purpose: The "advanced loans" module of the relatively new library software, ABCD, is an addition to the normal loans module and it offers a "generic transaction decision-making engine" functionality. The module requires extra installation effort and parameterisation, so this article aims to explain to the many potentially interested libraries,…

  8. Still a Deadly Disease? Performance Appraisal Systems in Academic Libraries in the United States

    Science.gov (United States)

    Stilling, Glenn Ellen Starr; Byrd, Allison S.; Mazza, Emily Rose; Bergman, Shawn M.

    2018-01-01

    Performance appraisal of professional librarians in academic libraries is important because of the critical role these employees play. Professional librarians ensure that the library's resources and services are effective, relevant, and integrated within the parent institution. Performance appraisal and job feedback have been understudied in the…

  9. Survey of Artificial Intelligence and Expert Systems in Library and Information Science Literature.

    Science.gov (United States)

    Hsieh, Cynthia C.; Hall, Wendy

    1989-01-01

    Examines the definition and history of artificial intelligence (AI) and investigates the body of literature on AI found in "Library Literature" and "Library and Information Science Abstracts." The results reported include the number of articles by year and per journal, and the percentage of articles dealing with library…

  10. Generation of neutron cross sections library for the Thermos code of the Fuel management System (FMS)

    International Nuclear Information System (INIS)

    Alonso V, G.; Viais J, J.

    1990-10-01

    There is developed a method to generate the library of neutron cross sections for the Thermos code by means of the database ENDF-B/IV and the NJOY code. The obtained results are compared with the version previous of the library of neutron cross sections which was processed using the version ENDF-B/III. (Author)

  11. Redesign of the C++ Communicating Threads Library for Embedded Control Systems

    NARCIS (Netherlands)

    Orlic, B.; Broenink, Johannes F.; Karelse, F.

    2004-01-01

    The occam programming language, based on the CSP formal algebra and tied to a specific hardware platform (transputers), offered a structured way to organize concurrency. The CT library [1], developed at University of Twente, inspired by occam is a kernel library implementing occam primitives in

  12. Automating the Technical Library at Los Angeles' Department of Information Systems.

    Science.gov (United States)

    Gillette, Robert

    1992-01-01

    Description of the automation of the technical library of the City of Los Angeles Department of Information Services provides background information on the department and its library; lists the automation project goals and objectives; and describes the two software programs--ObjectVision and Paradox Engine--used as applications development tools…

  13. Effects of the EVCAM chemical validation library on differentiation using marker gene expression in lmouse embryonic stem cells

    Science.gov (United States)

    The adherent cell differentiation and cytotoxicity (ACDC) assay was used to profile the effects of the ECVAM EST validation chemical library (19 compounds) on J1 mouse embryonic stem cells (mESC). PCR-based TaqMan Low Density Arrays (TLDA) provided a high-content assessment of al...

  14. Summary report from 1. research coordination meeting on nuclear data libraries for advance systems - fusion devices (FENDL - 3)

    International Nuclear Information System (INIS)

    Trkov, A.; Forrest, R.; Mengoni, A.

    2009-03-01

    The first Research Co-ordination Meeting of the Nuclear Data Libraries for Advance Systems - Fusion Devices (FENDL - 3) was held at the IAEA Headquarters in Vienna from 2 to 5 December 2008. A summary of the meeting is given in this report along with discussions which took place. An important outcome of the meeting was the agreement to create a new FENDL-3.0 Starter Library. Finally, a list of task assignments was prepared together with the plan for future CRP activities. (author)

  15. Nuclear Data Libraries for Advanced Systems - Fusion Devices (FENDL-3). Summary report from the Second Research Coordination Meeting

    International Nuclear Information System (INIS)

    Sawan, Mohamed E.

    2010-06-01

    The second Research Co-ordination Meeting of the Nuclear Data Libraries for Advanced Systems - Fusion Devices (FENDL - 3) was held at the IAEA Headquarters in Vienna from 23 to 26 March 2010. A summary of the meeting is given in this report along with the discussions which took place. An important outcome of the meeting was the decision to provide ENDF data libraries (FENDL-3/T) by April 2011. Finally, a list of task assignments was prepared together with the plan for future CRP activities. (author)

  16. Study on a self diagnostic monitoring system for an air-operated valve: development of a fault library

    International Nuclear Information System (INIS)

    Chai, Jang Bom; Kim, Yun Chul; Kim, Woo Shik; Cho, Hang Duke

    2004-01-01

    In the interest of nuclear power plant safety, a Self-Diagnostic Monitoring System (SDMS) is needed to monitor defects in safety-related components. An Air-Operated Valve (AOV) is one of the components to be monitored since the failure of its operation could potentially have catastrophic consequences. In this paper, a model of the AOV is developed with the parameters that affect the operational characteristics. The model is useful for both understanding the operation and correlating parameters and defects. Various defects are introduced in the experiments to construct a fault library, which will be used in a pattern recognition approach. Finally, the validity of the fault library is examined

  17. Economic Impact of the Hawaii State Public Library System (HSPLS) on the Business and Tourism Industries Study: Final Report

    Science.gov (United States)

    Ryan, Joe; McClure, Charles R.

    2003-01-01

    Ryan Information Management conducted a return on investment (ROI) study of the economic value of the Hawaii State Public Library System (HSPLS) and identified potential additional sources of operating revenue. HSPLS economic value was examined from four viewpoints, HSPLS: direct economic impact, market value, peer comparison and value to library…

  18. DSNP, Program and Data Library System for Dynamic Simulation of Nuclear Power Plant

    International Nuclear Information System (INIS)

    Saphier, D.; Madell, J.; Dean, E.

    1988-01-01

    1 - Description of problem or function: DSNP (Dynamic Simulator for Nuclear Power-Plants) is a system of programs and data files by which a nuclear power plant, or part thereof, can be simulated. The acronym DSNP is used interchangeably for the DSNP language, the DSNP libraries, the DSNP pre-compiler, and the DSNP document generator. The DSNP language is a special-purpose, block-oriented digital- simulation language developed to facilitate the preparation of dynamic simulations of a large variety of nuclear power plants. It is a user-oriented language that permits the user to prepare simulation programs directly from power plant block diagrams and flow charts by recognizing the symbolic DSNP statements for the appropriate physical components and listing these statements in a logical sequence according to the flow of physical properties in the simulated power plant. Physical components of nuclear power plants are represented by functional blocks, or modules. Many of the more complex components are represented by several modules. The nuclear reactor, for example, has a kinetic module, a power distribution module, a feedback module, a thermodynamic module, a hydraulic module, and a radioactive heat decay module. These modules are stored in DSNP libraries in the form of a DSNP subroutine or function, a block of statements, a macro, or a combination of the above. Basic functional blocks such as integrators, pipes, function generators, connectors, and many auxiliary functions representing properties of materials used in nuclear power plants are also available. The DSNP pre-compiler analyzes the DSNP simulation program, performs the appropriate translations, inserts the requested modules from the library, links these modules together, searches necessary data files, and produces a simulation program in FORTRAN. FORTRAN is considered to be a subset of DSNP and can be inserted anywhere in the simulation program without restriction. I/O statements can be located anywhere in

  19. Design and producing of fine-group cross section library HENDL3.0/FG for subcritical system

    International Nuclear Information System (INIS)

    Zou, J.; Zeng, Q.; Xu, D.; Hu, L.; Long, P.

    2012-01-01

    To improve the accuracy of the neutron analyses for subcritical system with thermal fission blanket, a coupled neutron and photon (315 n + 42γ) fine-group cross section library HENDL3.0/FG based on ENDF/B-VII, JEFF3.1 and JENDL3.3 was produced by FDS team. In order to test the availability and reliability of the HENDL3.0/FG data library, shielding and critical safety benchmarks were performed with VisualBUS code. The testing results indicated that the discrepancy between calculation and experimental values of nuclear parameters fell in a reasonable range. It showed that the nuclear data library had accuracy and availability. (authors)

  20. 圖書資訊系統的演變與發展 Evolutional Development of Library Information Systems

    Directory of Open Access Journals (Sweden)

    Hong-Chu Huang

    2010-06-01

    Full Text Available 圖書資訊系統是圖書館作為徵集、組織與服務圖書資訊資源的重要工具,隨著資訊技術的發展,圖書資訊系統也從傳統的卡片目錄逐漸蛻變成不同的多元樣貌,如整合式圖書館系統、聯合目錄、電子資源管理系統、亞馬遜網路書店與Google圖書等。本文旨在探討圖書資訊系統的歷史發展與未來方向,採取個案研究為方法,以主要趨勢的演變歷程為主軸,依序選取4類14個案進行討論,分析各式圖書資訊系統的特色,進而從物件類型、物件精細度、資源範圍、組織方式、聚合方式、聚合資源、呈現方式、軟體導入模式、社會化線上目錄、設計方式、運作方式與取用方式等12種觀點提出研究發現與建議。Library information systems are an essential tool for libraries to acquire and organize information resources to deliver services to users. With advancement of information technologies, library information systems have also evolved from card catalogue into diverse ones, such as integrated library system, elec- tronic resource management system, Amazon.com and Google Books. This article aims to review the historical development of library information systems as a basis to explore the future landscape of library information systems. Case study is adopted as research methodology to analyze fourteen library informa- tion systems. Furthermore, research findings and discussions are organized in the following points: type, granularity, boundary, organization and aggrega- tion of object, and representation of information resources, as well as software implementation, social OPAC, software design, operation model, and access.

  1. Synthetic promoter libraries for Corynebacterium glutamicum

    DEFF Research Database (Denmark)

    Rytter, Jakob Vang; Helmark, Søren; Chen, Jun

    2014-01-01

    The ability to modulate gene expression is an important genetic tool in systems biology and biotechnology. Here, we demonstrate that a previously published easy and fast PCR-based method for modulating gene expression in lactic acid bacteria is also applicable to Corynebacterium glutamicum. We co...... promoter library (SPL) technology is convenient for modulating gene expression in C. glutamicum and should have many future applications, within basic research as well as for optimizing industrial production organisms....... constructed constitutive promoter libraries based on various combinations of a previously reported C. glutamicum -10 consensus sequence (gngnTA(c/t)aaTgg) and the Escherichia coli -35 consensus, either with or without an AT-rich region upstream. A promoter library based on consensus sequences frequently found...... in low-GC Gram-positive microorganisms was also included. The strongest promoters were found in the library with a -35 region and a C. glutamicum -10 consensus, and this library also represents the largest activity span. Using the alternative -10 consensus TATAAT, which can be found in many other...

  2. Development of a distributed control system for TOTEM experiment using ASIO Boost C++ libraries

    Science.gov (United States)

    Cafagna, F.; Mercadante, A.; Minafra, N.; Quinto, M.; Radicioni, E.

    2014-06-01

    The main goals of the TOTEM Experiment at the LHC are the measurements of the elastic and total p-p cross sections and the studies of the diffractive dissociation processes. Those scientific objectives are achieved by using three tracking detectors symmetrically arranged around the interaction point called IP5. The control system is based on a C++ software that allows the user, by means of a graphical interface, direct access to hardware and handling of devices configuration. A first release of the software was designed as a monolithic block, with all functionalities being merged together. Such approach showed soon its limits, mainly poor reusability and maintainability of the source code, evident not only in phase of bug-fixing, but also when one wants to extend functionalities or apply some other modifications. This led to the decision of a radical redesign of the software, now based on the dialogue (message-passing) among separate building blocks. Thanks to the acquired extensibility, the software gained new features and now is a complete tool by which it is possible not only to configure different devices interfacing with a large subset of buses like I2C and VME, but also to do data acquisition both for calibration and physics runs. Furthermore, the software lets the user set up a series of operations to be executed sequentially to handle complex operations. To achieve maximum flexibility, the program units may be run either as a single process or as separate processes on different PCs which exchange messages over the network, thus allowing remote control of the system. Portability is ensured by the adoption of the ASIO (Asynchronous Input Output) library of Boost, a cross-platform suite of libraries which is candidate to become part of the C++ 11 standard. We present the state of the art of this project and outline the future perspectives. In particular, we describe the system architecture and the message-passing scheme. We also report on the results obtained

  3. Development of a radiation-responsive gene expression system

    International Nuclear Information System (INIS)

    Ogawa, Ryohei; Morii, Akihiro; Watanabe, Akihiko

    2013-01-01

    We have obtained a promoter enhancing expression of a gene of our interest connected downstream after activation in response to radiation stimulation and it could be used in radiogenetic therapy, a combination between radiotherapy and gene therapy. The promoter has been chosen out of a library of DNA fragments constructed by connecting the TATA box to randomly combined binding sequences of transcription factors that are activated in response to radiation. Although it was shown that the promoter activation was cell type specific, it turned out that radiation responsive promoters could be obtained for a different type of cells by using another set of transcription factor binding sequences, suggesting that the method would be feasible to obtain promoters functioning in any type of cells. Radiation reactivity of obtained promoters could be improved by techniques such as random introduction of point mutations. The improved promoters significantly enhanced expression of the luciferase gene connected downstream in response to radiation even in vivo, in addition, a gene cassette composed of one such promoter and the fcy::fur gene was confirmed useful for suicide gene therapy as shown in vitro simulation experiment, suggesting possible clinical application. (author)

  4. Usage Analysis for the Identification of Research Trends in Digital Libraries; Keepers of the Crumbling Culture: What Digital Preservation Can Learn from Library History; Patterns of Journal Use by Scientists through Three Evolutionary Phases; Developing a Content Management System-Based Web Site; Exploring Charging Models for Digital Cultural Heritage in Europe; Visions: The Academic Library in 2012.

    Science.gov (United States)

    Bollen, Johan; Vemulapalli, Soma Sekara; Xu, Weining; Luce, Rick; Marcum, Deanna; Friedlander, Amy; Tenopir, Carol; Grayson, Matt; Zhang, Yan; Ebuen, Mercy; King, Donald W.; Boyce, Peter; Rogers, Clare; Kirriemuir, John; Tanner, Simon; Deegan, Marilyn; Marcum, James W.

    2003-01-01

    Includes six articles that discuss use analysis and research trends in digital libraries; library history and digital preservation; journal use by scientists; a content management system-based Web site for higher education in the United Kingdom; cost studies for transitioning to digitized collections in European cultural institutions; and the…

  5. THE TERMINOLOGY OF LIBRARY SCIENCE

    OpenAIRE

    Љиљана Матић

    2014-01-01

    The master’s thesis entitled The Terminology of Library Science presents the general state of the terminology of library science in the Serbian language and analyses the terminological system which was formed in the last couple of decades in relation to library and information science. The terminology of library science is seen as a characteristic of professional language. The research is conducted on a corpus which excludes sources relating extremely to either library science or information ...

  6. Appraisal of the Effectiveness of CODE; The Coordinated Delivery System for the South Central Research Library Council, January to December 1970.

    Science.gov (United States)

    Faibisoff, Sylvia G.

    A major concern of the South Central Research Library Council in establishing an interlibrary loan network was the development of a Coordinated Delivery system (CODE). Several means of delivery were considered--the U.S. mails, commercial trucking (Greyhound, United Parcel Service), and use of the public library system's delivery services. A…

  7. Recent advances in PC-Linux systems for electronic structure computations by optimized compilers and numerical libraries.

    Science.gov (United States)

    Yu, Jen-Shiang K; Yu, Chin-Hui

    2002-01-01

    One of the most frequently used packages for electronic structure research, GAUSSIAN 98, is compiled on Linux systems with various hardware configurations, including AMD Athlon (with the "Thunderbird" core), AthlonMP, and AthlonXP (with the "Palomino" core) systems as well as the Intel Pentium 4 (with the "Willamette" core) machines. The default PGI FORTRAN compiler (pgf77) and the Intel FORTRAN compiler (ifc) are respectively employed with different architectural optimization options to compile GAUSSIAN 98 and test the performance improvement. In addition to the BLAS library included in revision A.11 of this package, the Automatically Tuned Linear Algebra Software (ATLAS) library is linked against the binary executables to improve the performance. Various Hartree-Fock, density-functional theories, and the MP2 calculations are done for benchmarking purposes. It is found that the combination of ifc with ATLAS library gives the best performance for GAUSSIAN 98 on all of these PC-Linux computers, including AMD and Intel CPUs. Even on AMD systems, the Intel FORTRAN compiler invariably produces binaries with better performance than pgf77. The enhancement provided by the ATLAS library is more significant for post-Hartree-Fock calculations. The performance on one single CPU is potentially as good as that on an Alpha 21264A workstation or an SGI supercomputer. The floating-point marks by SpecFP2000 have similar trends to the results of GAUSSIAN 98 package.

  8. Cloning and characterization of a novel human zinc finger gene, hKid3, from a C2H2-ZNF enriched human embryonic cDNA library

    International Nuclear Information System (INIS)

    Gao Li; Sun Chong; Qiu Hongling; Liu Hui; Shao Huanjie; Wang Jun; Li Wenxin

    2004-01-01

    To investigate the zinc finger genes involved in human embryonic development, we constructed a C 2 H 2 -ZNF enriched human embryonic cDNA library, from which a novel human gene named hKid3 was identified. The hKid3 cDNA encodes a 554 amino acid protein with an amino-terminal KRAB domain and 11 carboxyl-terminal C 2 H 2 zinc finger motifs. Northern blot analysis indicates that two hKid3 transcripts of 6 and 8.5 kb express in human fetal brain and kidney. The 6 kb transcript can also be detected in human adult brain, heart, and skeletal muscle while the 8.5 kb transcript appears to be embryo-specific. GFP-fused hKid3 protein is localized to nuclei and the ZF domain is necessary and sufficient for nuclear localization. To explore the DNA-binding specificity of hKid3, an oligonucleotide library was selected by GST fusion protein of hKid3 ZF domain, and the consensus core sequence 5'-CCAC-3' was evaluated by competitive electrophoretic mobility shift assay. Moreover, The KRAB domain of hKid3 exhibits transcription repressor activity when tested in GAL4 fusion protein assay. These results indicate that hKid3 may function as a transcription repressor with regulated expression pattern during human development of brain and kidney

  9. New management systems, new skills for librarians: implementing a process-oriented management system at the Library of the University of Almeria (Spain

    Directory of Open Access Journals (Sweden)

    Encarna Fuentes Melero

    2013-06-01

    Full Text Available In recent years, European Universities have been immersed in the search for formulas that meet the requirements of new management systems, based on the rationalization and assessment of both economic and human resources. Nowadays, the adaptation to these goals is a constant in the implementation of different management models, contributing to suit the patrons’ needs to the new environment: continuous analysis of working procedures, updating of librarian competencies and adjustment of new organizational structures to new methodologies. This paper presents a reflection about the changes that have taken place during the last decade at the Library of the University of Almeria (Spain, since the adoption of a process-oriented management system and the definition of the continuous assessment of working procedures. Concurrently, the abilities of the staff have been adjusted to the new situation, on the basis of the definition of the skills needed for the achievement of the library goals. It is described how new library services have been implemented through the analysis of the patrons’ needs: the Research Support service, or the Services and Resources Assessment service, have been set up in order to make the library budget more effective and rational. Librarians’ skills needed for these activities are analyzed, so the library staff can be appropriately trained, with the main objective of adjusting the Library goals, the librarian capabilities and the University needs through the performance evaluation of each position in this Service.

  10. Immune genes undergo more adaptive evolution than non-immune system genes in Daphnia pulex

    Directory of Open Access Journals (Sweden)

    McTaggart Seanna J

    2012-05-01

    Full Text Available Abstract Background Understanding which parts of the genome have been most influenced by adaptive evolution remains an unsolved puzzle. Some evidence suggests that selection has the greatest impact on regions of the genome that interact with other evolving genomes, including loci that are involved in host-parasite co-evolutionary processes. In this study, we used a population genetic approach to test this hypothesis by comparing DNA sequences of 30 putative immune system genes in the crustacean Daphnia pulex with 24 non-immune system genes. Results In support of the hypothesis, results from a multilocus extension of the McDonald-Kreitman (MK test indicate that immune system genes as a class have experienced more adaptive evolution than non-immune system genes. However, not all immune system genes show evidence of adaptive evolution. Additionally, we apply single locus MK tests and calculate population genetic parameters at all loci in order to characterize the mode of selection (directional versus balancing in the genes that show the greatest deviation from neutral evolution. Conclusions Our data are consistent with the hypothesis that immune system genes undergo more adaptive evolution than non-immune system genes, possibly as a result of host-parasite arms races. The results of these analyses highlight several candidate loci undergoing adaptive evolution that could be targeted in future studies.

  11. Screening a novel Na+/H+ antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China.

    Science.gov (United States)

    Xiang, Wenliang; Zhang, Jie; Li, Lin; Liang, Huazhong; Luo, Hai; Zhao, Jian; Yang, Zhirong; Sun, Qun

    2010-05-01

    Metagenomic DNA libraries constructed from the Dagong Ancient Brine Well were screened for genes with Na(+)/H(+) antiporter activity on the antiporter-deficient Escherichia coli KNabc strain. One clone with a stable Na(+)-resistant phenotype was obtained and its Na(+)/H(+) antiporter gene was sequenced and designated as m-nha. The deduced amino acid sequence of M-Nha protein consists of 523 residues with a calculated molecular weight of 58 147 Da and a pI of 5.50, which is homologous with NhaH from Halobacillus dabanensis D-8(T) (92%) and Halobacillus aidingensis AD-6(T) (86%), and with Nhe2 from Bacillus sp. NRRL B-14911 (64%). It had a hydropathy profile with 10 putative transmembrane domains and a long carboxyl terminal hydrophilic tail of 140 amino acid residues, similar to Nhap from Synechocystis sp. and Aphanothece halophytica, as well as NhaG from Bacillus subtilis. The m-nha gene in the antiporter-negative mutant E. coli KNabc conferred resistance to Na(+) and the ability to grow under alkaline conditions. The difference in amino acid sequence and the putative secondary structure suggested that the m-nha isolated from the Dagong Ancient Brine Well in this study was a novel Na(+)/H(+) antiporter gene.

  12. A universal phage display system for the seamless construction of Fab libraries.

    Science.gov (United States)

    Nelson, Renae S; Valadon, Philippe

    2017-11-01

    The construction of Fab phage libraries requires the cloning of domains from both the light and the heavy chain of antibodies. Despite the advent of powerful strategies such as splicing-by-overlap extension PCR, obtaining high quality libraries with excellent coverage remains challenging. Here, we explored the use of type IIS restriction enzymes for the seamless cloning of Fab libraries. We analyzed human, murine and rabbit germline antibody repertoires and identified combinations of restriction enzymes that exhibit very few or no recognition sites in the antibody sequences. We describe three phagemid vectors, pUP-22Hb, pUP-22Mc and pUP-22Rc, which were employed for cloning the Fab repertoire of these hosts using BsmBI and SapI (human) or SapI alone (mouse and rabbit). Using human serum albumin as a model immunization, we built a mouse/human chimeric Fab library and a mouse Fab library in a single step ligation and successfully panned multiple cognate antibodies. The overall process is highly scalable and faster than PCR-based techniques, with a Fab insertion success rate of around 80%. By using carefully chosen overhangs on each end of the antibody domains, this approach paves the way to the universal, sequence- and vector-independent cloning and reformatting of antibody libraries. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Coupled fvGCM-GCE Modeling System, 3D Cloud-Resolving Model and Cloud Library

    Science.gov (United States)

    Tao, Wei-Kuo

    2005-01-01

    Recent GEWEX Cloud System Study (GCSS) model comparison projects have indicated that cloud- resolving models (CRMs) agree with observations better than traditional single-column models in simulating various types of clouds and cloud systems from different geographic locations. Current and future NASA satellite programs can provide cloud, precipitation, aerosol and other data at very fine spatial and temporal scales. It requires a coupled global circulation model (GCM) and cloud-scale model (termed a super-parameterization or multi-scale modeling framework, MMF) to use these satellite data to improve the understanding of the physical processes that are responsible for the variation in global and regional climate and hydrological systems. The use of a GCM will enable global coverage, and the use of a CRM will allow for better and more sophisticated physical parameterization. NASA satellite and field campaign cloud related datasets can provide initial conditions as well as validation for both the MMF and CRMs. A seed fund is available at NASA Goddard to build a MMF based on the 2D Goddard Cumulus Ensemble (GCE) model and the Goddard finite volume general circulation model (fvGCM). A prototype MMF in being developed and production runs will be conducted at the beginning of 2005. In this talk, I will present: (1) A brief review on GCE model and its applications on precipitation processes, ( 2 ) The Goddard MMF and the major difference between two existing MMFs (CSU MMF and Goddard MMF), (3) A cloud library generated by Goddard MMF, and 3D GCE model, and (4) A brief discussion on the GCE model on developing a global cloud simulator.

  14. The impact of computerisation of library operations on library ...

    African Journals Online (AJOL)

    The use of computer-based systems in libraries and information units is now a vogue. The era of manual system in library operations is on its way to extinction. Recent developments in information world tend towards a globalized information communication technology (ICT). The library as a dynamic institution cannot afford ...

  15. Human gene therapy: novel approaches to improve the current gene delivery systems.

    Science.gov (United States)

    Cucchiarini, Magali

    2016-06-01

    Even though gene therapy made its way through the clinics to treat a number of human pathologies since the early years of experimental research and despite the recent approval of the first gene-based product (Glybera) in Europe, the safe and effective use of gene transfer vectors remains a challenge in human gene therapy due to the existence of barriers in the host organism. While work is under active investigation to improve the gene transfer systems themselves, the use of controlled release approaches may offer alternative, convenient tools of vector delivery to achieve a performant gene transfer in vivo while overcoming the various physiological barriers that preclude its wide use in patients. This article provides an overview of the most significant contributions showing how the principles of controlled release strategies may be adapted for human gene therapy.

  16. Gene expression programming for power system static security ...

    African Journals Online (AJOL)

    user

    Keywords: static security, gene expression programming, probabilistic neural network ... Hence digital computers are usually installed in operations control centers to gather ...... power system protection, and applications of AI in power systems.

  17. Retracted Association of STAT4 gene polymorphism with systemic lupus erythematosus / lupus nephritis risk.

    Science.gov (United States)

    Zhou, Tian-Biao; Jiang, Zong-Pei; Qin, Yuan-Han; Zhou, Jia-Fan

    2014-04-16

    The association of STAT4 gene polymorphism with systemic lupus erythematosus (SLE) / lupus nephritis (LN) results from the published studies is still conflicting. This meta-analysis was performed to evaluate the relationship between STAT4 rs7574865, rs16833431, rs11889341, rs8179673, rs10168266, rs7582694, rs3821236, rs7601754 gene polymorphism and SLE / LN, and to explore whether STAT4 gene polymorphism could become a predictive marker for SLE / LN risk. Association studies were identified from the databases of PubMed, Embase, Cochrane Library and CBM-disc (China Biological Medicine Database) as of September 1, 2013, and eligible investigations were synthesized using meta-analysis method. 24 investigations were identified for the analysis of association between STAT4 gene polymorphism and SLE, consisting of 31190 patients with SLE and 43940 controls. In STAT4 rs7574865, there was a marked association between T allele or TT genotype and SLE susceptibility (T: OR=1.53, 95% CI: 1.30-1.79, Prs7574865 gene polymorphism was not associated with the LN risk. Our results indicate that T allele or TT homozygous is a significant risk genetic molecular marker to predict the SLE susceptibility and GG genotype is a valuable marker to against the SLE risk, but the association was not found for LN. However, more investigations are required to further clarify the association of the T allele or TT homozygous with SLE / LN susceptibility. This article is protected by copyright. All rights reserved.

  18. Suppression subtractive hybridization library construction and identification of epidermal bladder cell related genes in the common ice plant, Mesembryanthemum crystallinum L.

    Directory of Open Access Journals (Sweden)

    Siranet Roeurn

    2016-10-01

    Full Text Available Mesembryanthemum crystallinum L., a halophytic species, displays modified trichomes, epidermal bladder cells (EBC, on the surfaces of its aerial organs. EBCs serve to sequester excessive salt from underlying metabolically active tissues. To elucidate the molecular determinants governing EBC development in the common ice plant, we constructed a cDNA-based suppression subtractive hybridization library and identified genes differentially expressed between the wild-type and the EBC-less mutant. After hybridization, 38 clones were obtained. Among them, 24 clones had homology with plant genes of known functions, whose roles might not be directly related to EBC-morphology, while 14 clones were homologous to genes of unknown functions. After confirmation by northern blot analysis, 12 out of 14 clones of unknown functions were chosen for semi-quantitative RT-PCR analysis, and the results revealed that three clones designated as MW3, MW21, and MW31 preferentially expressed in the EBC-less mutant, whereas the other two designated as WM10 and WM28 preferentially expressed in the wild type. Among these genes, the expression of a putative jasmonate-induced gene, designated as WM28 was completely suppressed in the EBC-mutant. In addition, the deletion of C-box cis-acting element was found in the promoter region of WM28 in the EBC-less mutant. Overexpression of WM28 in Arabidopsis resulted in increased trichome number due to the upregulation of key trichome-related genes GLABRA1 (GL1, and GLABRA3 (GL3. These results demonstrate that WM28 can be an important factor responsible for EBC formation, and also suggest the similarity of developmental mechanism between trichome in Arabidopsis and EBC in common ice plant.

  19. New Hyde Park Public Library; A Study.

    Science.gov (United States)

    Nassau Library System, Garden City, NY.

    In the fall of 1974, the Nassau County Library System conducted a study of the New Hyde Park Public Library at the request of that library's directing board. Relevant documents and statistics were reviewed, building usage was studied, and the library staff and community organizations were questioned. The library was analyzed in terms of the…

  20. Development of a Reference Image Collection Library for Histopathology Image Processing, Analysis and Decision Support Systems Research.

    Science.gov (United States)

    Kostopoulos, Spiros; Ravazoula, Panagiota; Asvestas, Pantelis; Kalatzis, Ioannis; Xenogiannopoulos, George; Cavouras, Dionisis; Glotsos, Dimitris

    2017-06-01

    Histopathology image processing, analysis and computer-aided diagnosis have been shown as effective assisting tools towards reliable and intra-/inter-observer invariant decisions in traditional pathology. Especially for cancer patients, decisions need to be as accurate as possible in order to increase the probability of optimal treatment planning. In this study, we propose a new image collection library (HICL-Histology Image Collection Library) comprising 3831 histological images of three different diseases, for fostering research in histopathology image processing, analysis and computer-aided diagnosis. Raw data comprised 93, 116 and 55 cases of brain, breast and laryngeal cancer respectively collected from the archives of the University Hospital of Patras, Greece. The 3831 images were generated from the most representative regions of the pathology, specified by an experienced histopathologist. The HICL Image Collection is free for access under an academic license at http://medisp.bme.teiath.gr/hicl/ . Potential exploitations of the proposed library may span over a board spectrum, such as in image processing to improve visualization, in segmentation for nuclei detection, in decision support systems for second opinion consultations, in statistical analysis for investigation of potential correlations between clinical annotations and imaging findings and, generally, in fostering research on histopathology image processing and analysis. To the best of our knowledge, the HICL constitutes the first attempt towards creation of a reference image collection library in the field of traditional histopathology, publicly and freely available to the scientific community.

  1. Kmerind: A Flexible Parallel Library for K-mer Indexing of Biological Sequences on Distributed Memory Systems.

    Science.gov (United States)

    Pan, Tony; Flick, Patrick; Jain, Chirag; Liu, Yongchao; Aluru, Srinivas

    2017-10-09

    Counting and indexing fixed length substrings, or k-mers, in biological sequences is a key step in many bioinformatics tasks including genome alignment and mapping, genome assembly, and error correction. While advances in next generation sequencing technologies have dramatically reduced the cost and improved latency and throughput, few bioinformatics tools can efficiently process the datasets at the current generation rate of 1.8 terabases every 3 days. We present Kmerind, a high performance parallel k-mer indexing library for distributed memory environments. The Kmerind library provides a set of simple and consistent APIs with sequential semantics and parallel implementations that are designed to be flexible and extensible. Kmerind's k-mer counter performs similarly or better than the best existing k-mer counting tools even on shared memory systems. In a distributed memory environment, Kmerind counts k-mers in a 120 GB sequence read dataset in less than 13 seconds on 1024 Xeon CPU cores, and fully indexes their positions in approximately 17 seconds. Querying for 1% of the k-mers in these indices can be completed in 0.23 seconds and 28 seconds, respectively. Kmerind is the first k-mer indexing library for distributed memory environments, and the first extensible library for general k-mer indexing and counting. Kmerind is available at https://github.com/ParBLiSS/kmerind.

  2. Library Computing

    Science.gov (United States)

    Library Computing, 1985

    1985-01-01

    Special supplement to "Library Journal" and "School Library Journal" covers topics of interest to school, public, academic, and special libraries planning for automation: microcomputer use, readings in automation, online searching, databases of microcomputer software, public access to microcomputers, circulation, creating a…

  3. Trends in Special Library Buildings.

    Science.gov (United States)

    Cohen, Elaine; Cohen, Aaron

    1987-01-01

    Examines special library facilities, noting impact of organizational structure, and discusses the concept of information resources management in this context. Development of online and telecommunications systems, corporate and government campuses, and special library systems are identified as reasons for growth of special libraries. Furniture and…

  4. LIBRARY AUTOMATION IN NIGERAN UNIVERSITIES

    African Journals Online (AJOL)

    facilitate services and access to information in libraries is widely acceptable. ... Moreover, Ugah (2001) reports that the automation process at the. Abubakar ... blueprint in 1987 and a turn-key system of automation was suggested for the library.

  5. The computer library of experimental neutron data

    International Nuclear Information System (INIS)

    Bychkov, V.M.; Manokhin, V.N.; Surgutanov, V.V.

    1976-05-01

    The paper describes the computer library of experimental neutron data at the Obninsk Nuclear Data Centre. The format of the library (EXFOR) and the system of programmes for supplying the library are briefly described. (author)

  6. Control of gene expression by CRISPR-Cas systems

    Science.gov (United States)

    2013-01-01

    Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems. PMID:24273648

  7. Iowa DNR - NRGIS Library

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  8. A two-cassette reporter system for assessing target gene translation and target gene product inclusion body formation

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention relates to a dual cassette reporter system capable of assessing target gene translation and target gene product folding. The present invention further relates to vectors and host cells comprising the dual cassette reporter system. In addition the invention relates to the use...... of the dual cassette reporter system for assessing target gene translation and target gene product folding....

  9. The Journal of Earth System Science Education: Peer Review for Digital Earth and Digital Library Content

    Science.gov (United States)

    Johnson, D.; Ruzek, M.; Weatherley, J.

    2001-05-01

    published on the JESSE web server, ensuring long term access to the resource as reviewed. JESSE is collaborating with the Digital Library for Earth System Education (DLESE) as a federated partner. Initial release is planned for Summer, 2001.

  10. Generating Collaborative Systems for Digital Libraries: a Model-Driven Approach

    Directory of Open Access Journals (Sweden)

    Alessio Malizia

    2010-12-01

    Full Text Available The design and development of a digital library involves different stakeholders, such as: information architects, librarians, and domain experts, who need to agree on a common language to describe, discuss, and negotiate the services the library has to offer. To this end, high-level, language-neutral models have to be devised. Metamodeling techniques favor the definition of domainspecific visual languages through which stakeholders can share their views and directly manipulate representations of the domain entities. This paper describes CRADLE (Cooperative-Relational Approach to Digital Library Environments, a metamodel-based framework and visual language for the definition of notions and services related to the development of digital libraries. A collection of tools allows the automatic generation of several services, defined with the CRADLE visual language, and of the graphical user interfaces providing access to them for the final user. The effectiveness of the approach is illustrated by presenting digital libraries generated with CRADLE, while the CRADLE environment has been evaluated by using the cognitive dimensions framework.

  11. Description of the ENDF-NJOY system for the generation of cross sections libraries

    International Nuclear Information System (INIS)

    Alonso V, G.

    1991-01-01

    The physics of nuclear reactors requires of a great number of data to be able to evaluate the different phenomena that happen in a nuclear reactor; these data are mainly the microscopic neutron cross sections, but it is also required of data of radioactive decay and of nuclear structure for a great number of materials as well as of the cross sections of the photons and the production of these for the neutron interaction. These data group in nuclear databases, being the main ones: ENDF Nuclear Evaluated File, ENDL Dates Nuclear Evaluated Library it Dates (of the Laboratory Lawrence Livermore). JENDL Japanese Nuclear Evaluated Library Dates. Soviet SOKRATOR Nuclear Evaluated KEDAF Nuclear Karlsruhe File Dates. JEF Join Evaluated File (coordinated by NEA Data Bank). The existent codes that execute neutron and photon calculations require libraries of data that are very different some of other and of the databases. Of here that it is required of a series of processing codes that use the database like enter and its generate a secondary library of cross sections, which is read as enter for a code of spectra generation. Generally average cross sections by group are obtained; this library is that it is used in the codes that execute neutron calculations. (Author)

  12. XML in Libraries.

    Science.gov (United States)

    Tennant, Roy, Ed.

    This book presents examples of how libraries are using XML (eXtensible Markup Language) to solve problems, expand services, and improve systems. Part I contains papers on using XML in library catalog records: "Updating MARC Records with XMLMARC" (Kevin S. Clarke, Stanford University) and "Searching and Retrieving XML Records via the…

  13. Gene Therapy with the Sleeping Beauty Transposon System.

    Science.gov (United States)

    Kebriaei, Partow; Izsvák, Zsuzsanna; Narayanavari, Suneel A; Singh, Harjeet; Ivics, Zoltán

    2017-11-01

    The widespread clinical implementation of gene therapy requires the ability to stably integrate genetic information through gene transfer vectors in a safe, effective, and economical manner. The latest generation of Sleeping Beauty (SB) transposon vectors fulfills these requirements, and may overcome limitations associated with viral gene transfer vectors and transient nonviral gene delivery approaches that are prevalent in ongoing clinical trials. The SB system enables high-level stable gene transfer and sustained transgene expression in multiple primary human somatic cell types, thereby representing a highly attractive gene transfer strategy for clinical use. Here, we review the most important aspects of using SB for gene therapy, including vectorization as well as genomic integration features. We also illustrate the path to successful clinical implementation by highlighting the application of chimeric antigen receptor (CAR)-modified T cells in cancer immunotherapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. The shortage of superhero comic books in academic libraries: Case study of the Library System of the University of Puerto Rico at Río Piedras

    Directory of Open Access Journals (Sweden)

    Manuel Martínez Nazario

    2015-07-01

    Full Text Available Objective. The objective of this article has been to prove the findings about the lack of superhero comic books in an academic library in Puerto Rico, as in to demostrate its academic value of this bibliographical resource, and the possibility of integration. Method. For this qualitative study, it has been consulted the related literature, and it has been used interviews, with the intention to explore the reasons of this phenomenon. Results. The shortage of superhero comic books is connected with the lack of curricular justification and the absence of recommendations for acquisition. Conclusions. The superhero comic books have demostrated a great popularity among the readers and have comfirmed their potential in the academic researches. The scope of this information source has surpassed the boundaries of entertainment and has been incorporated to school libraries, public libraries and academic libraries.

  15. Automatic Generation of Agents using Reusable Soft Computing Code Libraries to develop Multi Agent System for Healthcare

    OpenAIRE

    Priti Srinivas Sajja

    2015-01-01

    This paper illustrates architecture for a multi agent system in healthcare domain. The architecture is generic and designed in form of multiple layers. One of the layers of the architecture contains many proactive, co-operative and intelligent agents such as resource management agent, query agent, pattern detection agent and patient management agent. Another layer of the architecture is a collection of libraries to auto-generate code for agents using soft computing techni...

  16. Benchmarks solutions of the coupled systems NJOY/AMPX-II/HAMMER-TECHNION and the library JENDL-3

    International Nuclear Information System (INIS)

    Santos, A. dos

    1991-01-01

    Benchmarks calculations were performed with the newest released japanese nuclear data library JENDL-3. The calculations were performed with the methodology developed at IPEN/CNEN-SP based on a coupled NJOY/AMPX-II/HAMMER-TECHNION system. The analyses show that the long-standing problem of the overprediction of the epithermal U-238 neutron capture still remains. Besides that there is an indication that the fission cross section of U-238 might be underestimated. (author)

  17. Generation of mariner-based transposon insertion mutant library of Bacillus sphaericus 2297 and investigation of genes involved in sporulation and mosquito-larvicidal crystal protein synthesis.

    Science.gov (United States)

    Wu, Yiming; Hu, Xiaomin; Ge, Yong; Zheng, Dasheng; Yuan, Zhiming

    2012-05-01

    Bacillus sphaericus has been used with great success in mosquito control programs worldwide. Under conditions of nutrient limitation, it undergoes sporulation via a series of well defined morphological stages. However, only a small number of genes involved in sporulation have been identified. To identify genes associated with sporulation, and to understand the relationship between sporulation and crystal protein synthesis, a random mariner-based transposon insertion mutant library of B. sphaericus strain 2297 was constructed and seven sporulation-defective mutants were selected. Sequencing of the DNA flanking of the transposon insertion identified several genes involved in sporulation. The morphologies of mutants were determined by electron microscopy and synthesis of crystal proteins was analyzed by SDS-PAGE and Western blot. Four mutants blocked at early stages of sporulation failed to produce crystal proteins and had lower larvicidal activity. However, the other three mutants were blocked at later stages and were able to form crystal proteins, and the larvicidal activity was similar to wild type. These results indicated that crystal protein synthesis in B. sphaericus is dependent on sporulation initiation. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  18. Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes*

    Science.gov (United States)

    Hu, Qing-bi; He, Yu; Zhou, Xun

    2015-01-01

    Species included in the Sporothrix schenckii complex are temperature-dependent with dimorphic growth and cause sporotrichosis that is characterized by chronic and fatal lymphocutaneous lesions. The putative species included in the Sporothrix complex are S. brasiliensis, S. globosa, S. mexicana, S. pallida, S. schenckii, and S. lurei. S. globosa is the causal agent of sporotrichosis in China, and its pathogenicity appears to be closely related to the dimorphic transition, i.e. from the mycelial to the yeast phase, it adapts to changing environmental conditions. To determine the molecular mechanisms of the switching process that mediates the dimorphic transition of S. globosa, suppression subtractive hybridization (SSH) was used to prepare a complementary DNA (cDNA) subtraction library from the yeast and mycelial phases. Bioinformatics analysis was performed to profile the relationship between differently expressed genes and the dimorphic transition. Two genes that were expressed at higher levels by the yeast form were selected, and their differential expression levels were verified using a quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). It is believed that these differently expressed genes are involved in the pathogenesis of S. globosa infection in China. PMID:26642182

  19. A new tetracycline efflux gene, tet(40), is located in tandem with tet(O/32/O) in a human gut firmicute bacterium and in metagenomic library clones.

    Science.gov (United States)

    Kazimierczak, Katarzyna A; Rincon, Marco T; Patterson, Andrea J; Martin, Jennifer C; Young, Pauline; Flint, Harry J; Scott, Karen P

    2008-11-01

    The bacterium Clostridium saccharolyticum K10, isolated from a fecal sample obtained from a healthy donor who had received long-term tetracycline therapy, was found to carry three tetracycline resistance genes: tet(W) and the mosaic tet(O/32/O), both conferring ribosome protection-type resistance, and a novel, closely linked efflux-type resistance gene designated tet(40). tet(40) encodes a predicted membrane-associated protein with 42% amino acid identity to tetA(P). Tetracycline did not accumulate in Escherichia coli cells expressing the Tet(40) efflux protein, and resistance to tetracycline was reduced when cells were incubated with an efflux pump inhibitor. E. coli cells carrying tet(40) had a 50% inhibitory concentration of tetracycline of 60 microg/ml. Analysis of a transconjugant from a mating between donor strain C. saccharolyticum K10 and the recipient human gut commensal bacterium Roseburia inulinivorans suggested that tet(O/32/O) and tet(40) were cotransferred on a mobile element. Sequence analysis of a 37-kb insert identified on the basis of tetracycline resistance from a metagenomic fosmid library again revealed a tandem arrangement of tet(O/32/O) and tet(40), flanked by regions with homology to parts of the VanG operon previously identified in Enterococcus faecalis. At least 10 of the metagenomic inserts that carried tet(O/32/O) also carried tet(40), suggesting that tet(40), although previously undetected, may be an abundant efflux gene.

  20. Molecular Cloning and Characterization of a Newly Isolated Pyrethroid-Degrading Esterase Gene from a Genomic Library of Ochrobactrum anthropi YZ-1

    Science.gov (United States)

    Song, Jinlong; Shi, Yanhua; Li, Kang; Zhao, Bin; Yan, Yanchun

    2013-01-01

    A novel pyrethroid-degrading esterase gene pytY was isolated from the genomic library of Ochrobactrum anthropi YZ-1. It possesses an open reading frame (ORF) of 897 bp. Blast search showed that its deduced amino acid sequence shares moderate identities (30% to 46%) with most homologous esterases. Phylogenetic analysis revealed that PytY is a member of the esterase VI family. pytY showed very low sequence similarity compared with reported pyrethroid-degrading genes. PytY was expressed, purified, and characterized. Enzyme assay revealed that PytY is a broad-spectrum degrading enzyme that can degrade various pyrethroids. It is a new pyrethroid-degrading gene and enriches genetic resource. Kinetic constants of Km and Vmax were 2.34 mmol·L−1 and 56.33 nmol min−1, respectively, with lambda-cyhalothrin as substrate. PytY displayed good degrading ability and stability over a broad range of temperature and pH. The optimal temperature and pH were of 35°C and 7.5. No cofactors were required for enzyme activity. The results highlighted the potential use of PytY in the elimination of pyrethroid residuals from contaminated environments. PMID:24155944

  1. The Personal Virtual Library

    CERN Document Server

    Le Meur, Jean-Yves

    1998-01-01

    Looking for "library" in the usual search engines of the World Wide Web gives: "Infoseek found 3,593,126 pages containing the word library" and it nicely proposes: "Search only within these 3,59 3,126 pages ?" "Yahoo! Found 1299 categories and 8669 sites for library" "LycOs: 1-10 von 512354 relevanten Ergebnissen" "AltaVista: About 14830527 documents match your query" and at the botto m: "Word count: library: 15466897" ! Excite: Top 10 matches and it does not say how many can be browsed... "Library" on the World Wide Web is really popular. At least fiveteen million pages ar e supposed to contain this word. Half of them may have disappeared by now but one more hit will be added once the search robots will have indexed this document ! The notion of Personal Library i s a modest attempt, in a small environment like a library, to give poor users lost in cyber-libraries the opportunity to keep their own private little shelves - virtually. In this paper, we will l ook at the usual functionalities of library systems...

  2. The first report: An analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16S ribosomal RNA gene-based clone library method.

    Science.gov (United States)

    You, Chunlin; Hamasuna, Ryoichi; Ogawa, Midori; Fukuda, Kazumasa; Hachisuga, Toru; Matsumoto, Tetsuro; Taniguchi, Hatsumi

    2016-06-01

    To analyse the bacterial flora of urine from patients with male urethritis using the clone library method. Urine specimens from patients with urethritis were used. The bacterial flora was analysed according to the 16S ribosomal RNA gene-based clone library method. In addition, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum or Ureaplasma parvum were detected by the conventional PCR methods (TMA or real-time PCR) and data from the clone library and conventional PCR methods were compared. Among 58 urine specimens, 38 were successfully analysed using the clone library method. From the specimens, 2427 clones were evaluated and 95 bacterial phylotypes were detected. N. gonorrhoeae was detected from 6 specimens and as the predominant bacterial species in 5 specimens. M. genitalium was detected from 5 specimens and as the predominant bacterial species in 3 specimens. C. trachomatis was detected from 15 specimens using the TMA method, but was detected from only 1 specimen using the clone library method. U. parvum was detected from only 2 specimens using the clone library method. In addition, Haemophilus influenzae and Neisseria meningitidis were also detected in 8 and 1 specimens, respectively. Gardnerella vaginalis, which is a potential pathogen for bacterial vaginitis in women, was detected in 10 specimens. The clone library method can detect the occupancy rate of each bacteria species among the bacterial flora and may be a new method for bacterial analyses in male urethritis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Seasonal diversity of planktonic protists in Southwestern Alberta rivers over a 1-year period as revealed by terminal restriction fragment length polymorphism and 18S rRNA gene library analyses.

    Science.gov (United States)

    Thomas, Matthew C; Selinger, L Brent; Inglis, G Douglas

    2012-08-01

    The temporal dynamics of planktonic protists in river water have received limited attention despite their ecological significance and recent studies linking phagotrophic protists to the persistence of human-pathogenic bacteria. Using molecular-based techniques targeting the 18S rRNA gene, we studied the seasonal diversity of planktonic protists in Southwestern Alberta rivers (Oldman River Basin) over a 1-year period. Nonmetric multidimensional scaling analysis of terminal restriction fragment length polymorphism (T-RFLP) data revealed distinct shifts in protistan community profiles that corresponded to season rather than geographical location. Community structures were examined by using clone library analysis; HaeIII restriction profiles of 18S rRNA gene amplicons were used to remove prevalent solanaceous plant clones prior to sequencing. Sanger sequencing of the V1-to-V3 region of the 18S rRNA gene libraries from spring, summer, fall, and winter supported the T-RFLP results and showed marked seasonal differences in the protistan community structure. The spring library was dominated by Chloroplastidae (29.8%), Centrohelida (28.1%), and Alveolata (25.5%), while the summer and fall libraries contained primarily fungal clones (83.0% and 88.0%, respectively). Alveolata (35.6%), Euglenozoa (24.4%), Chloroplastida (15.6%), and Fungi (15.6%) dominated the winter library. These data demonstrate that planktonic protists, including protozoa, are abundant in river water in Southwestern Alberta and that conspicuous seasonal shifts occur in the community structure.

  4. 2009 Capacity Building For Library and Information Centre

    African Journals Online (AJOL)

    discussed the features of digitized/virtual library and information centre and the need for capacity building especially in .... electronic library, virtual reality, digital library, community library .... architecture of the existing information system and ...

  5. Evaluating Public Libraries Using Standard Scores: The Library Quotient.

    Science.gov (United States)

    O'Connor, Daniel O.

    1982-01-01

    Describes a method for assessing the performance of public libraries using a standardized scoring system and provides an analysis of public library data from New Jersey as an example. Library standards and the derivation of measurement ratios are also discussed. A 33-item bibliography and three data tables are included. (JL)

  6. The Changing Environment and Changing Institution: Indian Project of the Northeast Kansas Library System

    Science.gov (United States)

    Cunningham, William D.

    1971-01-01

    The chief features of the program's first year were the establishment of a liason between the library and identifiable service needs within the community, the second year was for developing the communication center approach; and development of special services and a cultural awareness program were projected for the third and fourth years. (2…

  7. Design and implementation of universal mathematical library supporting algorithm development for FPGA based systems in high energy physics experiments

    International Nuclear Information System (INIS)

    Jalmuzna, W.

    2006-02-01

    The X-ray free-electron laser XFEL that is being planned at the DESY research center in cooperation with European partners will produce high-intensity ultra-short Xray flashes with the properties of laser light. This new light source, which can only be described in terms of superlatives, will open up a whole range of new perspectives for the natural sciences. It could also offer very promising opportunities for industrial users. SIMCON (SIMulator and CONtroller) is the project of the fast, low latency digital controller dedicated for LLRF system in VUV FEL experiment based on modern FPGA chips It is being developed by ELHEP group in Institute of Electronic Systems at Warsaw University of Technology. The main purpose of the project is to create a controller for stabilizing the vector sum of fields in cavities of one cryomodule in the experiment. The device can be also used as the simulator of the cavity and testbench for other devices. Flexibility and computation power of this device allow implementation of fast mathematical algorithms. This paper describes the concept, implementation and tests of universal mathematical library for FPGA algorithm implementation. It consists of many useful components such as IQ demodulator, division block, library for complex and floating point operations, etc. It is able to speed up implementation time of many complicated algorithms. Library have already been tested using real accelerator signals and the performance achieved is satisfactory. (Orig.)

  8. Design and implementation of universal mathematical library supporting algorithm development for FPGA based systems in high energy physics experiments

    Energy Technology Data Exchange (ETDEWEB)

    Jalmuzna, W.

    2006-02-15

    The X-ray free-electron laser XFEL that is being planned at the DESY research center in cooperation with European partners will produce high-intensity ultra-short Xray flashes with the properties of laser light. This new light source, which can only be described in terms of superlatives, will open up a whole range of new perspectives for the natural sciences. It could also offer very promising opportunities for industrial users. SIMCON (SIMulator and CONtroller) is the project of the fast, low latency digital controller dedicated for LLRF system in VUV FEL experiment based on modern FPGA chips It is being developed by ELHEP group in Institute of Electronic Systems at Warsaw University of Technology. The main purpose of the project is to create a controller for stabilizing the vector sum of fields in cavities of one cryomodule in the experiment. The device can be also used as the simulator of the cavity and testbench for other devices. Flexibility and computation power of this device allow implementation of fast mathematical algorithms. This paper describes the concept, implementation and tests of universal mathematical library for FPGA algorithm implementation. It consists of many useful components such as IQ demodulator, division block, library for complex and floating point operations, etc. It is able to speed up implementation time of many complicated algorithms. Library have already been tested using real accelerator signals and the performance achieved is satisfactory. (Orig.)

  9. Gene annotation from scientific literature using mappings between keyword systems.

    Science.gov (United States)

    Pérez, Antonio J; Perez-Iratxeta, Carolina; Bork, Peer; Thode, Guillermo; Andrade, Miguel A

    2004-09-01

    The description of genes in databases by keywords helps the non-specialist to quickly grasp the properties of a gene and increases the efficiency of computational tools that are applied to gene data (e.g. searching a gene database for sequences related to a particular biological process). However, the association of keywords to genes or protein sequences is a difficult process that ultimately implies examination of the literature related to a gene. To support this task, we present a procedure to derive keywords from the set of scientific abstracts related to a gene. Our system is based on the automated extraction of mappings between related terms from different databases using a model of fuzzy associations that can be applied with all generality to any pair of linked databases. We tested the system by annotating genes of the SWISS-PROT database with keywords derived from the abstracts linked to their entries (stored in the MEDLINE database of scientific references). The performance of the annotation procedure was much better for SWISS-PROT keywords (recall of 47%, precision of 68%) than for Gene Ontology terms (recall of 8%, precision of 67%). The algorithm can be publicly accessed and used for the annotation of sequences through a web server at http://www.bork.embl.de/kat

  10. System Biology Approach: Gene Network Analysis for Muscular Dystrophy.

    Science.gov (United States)

    Censi, Federica; Calcagnini, Giovanni; Mattei, Eugenio; Giuliani, Alessandro

    2018-01-01

    Phenotypic changes at different organization levels from cell to entire organism are associated to changes in the pattern of gene expression. These changes involve the entire genome expression pattern and heavily rely upon correlation patterns among genes. The classical approach used to analyze gene expression data builds upon the application of supervised statistical techniques to detect genes differentially expressed among two or more phenotypes (e.g., normal vs. disease). The use of an a posteriori, unsupervised approach based on principal component analysis (PCA) and the subsequent construction of gene correlation networks can shed a light on unexpected behaviour of gene regulation system while maintaining a more naturalistic view on the studied system.In this chapter we applied an unsupervised method to discriminate DMD patient and controls. The genes having the highest absolute scores in the discrimination between the groups were then analyzed in terms of gene expression networks, on the basis of their mutual correlation in the two groups. The correlation network structures suggest two different modes of gene regulation in the two groups, reminiscent of important aspects of DMD pathogenesis.

  11. Availability and Reliability of Disaster Early Warning Systems and the IT Infrastructure Library

    Science.gov (United States)

    Wächter, J.; Loewe, P.

    2012-12-01

    Decision Processes in Evolving Crises) funded under the European Union's seventh Framework Programme (FP7). This ongoing project focuses on real-time intelligent information management in Earth management and its long-term application. All technical development in TRIDEC is based on mature system architecture models and industry standards. The use of standards applies also to the operation of individual TRIDEC reference installations and their interlinking into an integrated service infrastructure for supranational warning services: A set of best practices for IT service management is used to align the TEWS software services with the requirements by the Early Warning Centre management by defining Service Level Agreements (SLA) and ensuring appliance. For this, the concept of service lifecycles is adapted for the TEWS domain, which is laid out in the IT Infrastructure Library (ITIL) by the United Kingdom's Office of Government Commerce (OGC). The cyclic procedures, tasks and checklists described by ITIL are used to establish a baseline to plan, implement, and maintain TEWS service components in the long run. This allows to ensure compliance with given international TEWS standards and to measure improvement of the provided services against a gold-standard.

  12. Efek Moderasi Tingkat Pendidikan Pada Delone & Mclean Information System Success Model (Studi Kasus Digital Library Universitas Gadjah Mada (UGM))

    OpenAIRE

    Wicaksana, Iyon Sukma; Hartanto, Rudy; Nugroho, Lukito Edi

    2017-01-01

    Digital library Universitas Gadjah Mada merupakan layanan yang diberikan kepada mahasiswa untuk mempermudah dalam mengakses informasi/referensi. Terdapat beberapa faktor terkait yang mempengaruhi suksesnya penerapan digital library. Keberadaan faktor-faktor tersebut menjadi penting untuk diketahui sejauh mana kesuksesan penerapan digital library. Dengan demikian diharapkan fasilitas digital library digunakan secara optimum oleh mahasiswa.Penelitian ini akan melakukan penelitian efek moderasi ...

  13. Insect-gene-activity detection system for chemical and biological warfare agents and toxic industrial chemicals

    Science.gov (United States)

    Mackie, Ryan S.; Schilling, Amanda S.; Lopez, Arturo M.; Rayms-Keller, Alfredo

    2002-02-01

    Detection of multiple chemical and biological weapons (CBW) agents and/or complex mixtures of toxic industrial chemicals (TIC) is imperative for both the commercial and military sectors. In a military scenario, a multi-CBW attack would create confusion, thereby delaying decontamination and therapeutic efforts. In the commercial sector, polluted sites invariably contain a mixture of TIC. Novel detection systems capable of detecting CBW and TIC are sorely needed. While it may be impossible to build a detector capable of discriminating all the possible combinations of CBW, a detection system capable of statistically predicting the most likely composition of a given mixture is within the reach of current emerging technologies. Aquatic insect-gene activity may prove to be a sensitive, discriminating, and elegant paradigm for the detection of CBW and TIC. We propose to systematically establish the expression patterns of selected protein markers in insects exposed to specific mixtures of chemical and biological warfare agents to generate a library of biosignatures of exposure. The predicting capabilities of an operational library of biosignatures of exposures will allow the detection of emerging novel or genetically engineered agents, as well as complex mixtures of chemical and biological weapons agents. CBW and TIC are discussed in the context of war, terrorism, and pollution.

  14. Nanobarcode gene expression monitoring system for potential miniaturized space applications

    Science.gov (United States)

    Ruan, Weiming; Eastman, P. Scott; Cooke, Patrick A.; Park, Jennifer S.; Chu, Julia S. F.; Gray, Joe W.; Li, Song; Chen, Fanqing Frank

    Manned mission to space has been threatened by various cosmos risks including radiation, mirogravity, vacuum, confinement, etc., which may cause genetic variations of astronauts and eventually lead to damages of their health. Thus, the development of small biomedical devices, which can monitor astronaut gene expression changes, is useful for future long-term space missions. Using magnetic microbeads packed with nanocrystal quantum dots at controlled ratios, we were able to generate highly multiplexed nanobarcodes, which can encode a flexible panel of genes. Also, by using a reporter quantum dot, this nanobarcode platform can monitor and quantify gene expression level with improved speed and sensitivity. As a comparison, we studied TGF-β1 induced transcription changes in human bone marrow mesenchymal stem cells with both the nanobarcode microbead system and the Affymetrix GeneChip ® HTA system, which is currently considered as the industrial standard. Though using only 1/20 of the sample RNA, the nanobarcode system showed sensitivity equivalent to Affymetrix GeneChip ® system. The coefficient of variation, dynamic range, and accuracy of the nanobarcodes measurement is equivalent to that of the GeneChip ® HTA system. Therefore, this newly invented nanobarcode microbead platform is thought to be sensitive, flexible, cost-effective and accurate in a level equivalent to the conventional methods. As an extension of the use of this new platform, spacecrafts may carry this miniaturized system as a diagnostic tool for the astronauts.

  15. More library mashups exploring new ways to deliver library data

    CERN Document Server

    2015-01-01

    Nicole Engard follows up her ground-breaking 2009 book Library Mashups with a fresh collection of mashup projects that virtually any library can emulate, customize, and build upon. In More Library Mashups, Engard and 24 creative library professionals describe how they are mashing up free and inexpensive digital tools and techniques to improve library services and meet everyday (and unexpected) challenges. Examples from libraries of all types are designed to help even non-programmers share and add value to digital content, update and enhance library websites and collections, mashup catalog data, connect to the library's automation system, and use emerging tools like Serendip-o-matic, Umlaut, and Libki to engage users, staff, and the community.

  16. Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40.

    Science.gov (United States)

    Rampuria, Sakshi; Joshi, Uma; Palit, Paramita; Deokar, Amit A; Meghwal, Raju R; Mohapatra, T; Srinivasan, R; Bhatt, K V; Sharma, Ramavtar

    2012-11-01

    Moth bean ( Vigna aconitifolia (Jacq.) Marechal) is an important grain legume crop grown in rain fed areas of hot desert regions of Thar, India, under scorching sun rays with very little supplementation of water. An SSH cDNA library was generated from leaf tissues of V. aconitifolia var. RMO-40 exposed to an elevated temperature of 42 °C for 5 min to identify early-induced genes. A total of 488 unigenes (114 contigs and 374 singletons) were derived by cluster assembly and sequence alignment of 738 ESTs; out of 206 ESTs (28%) of unknown proteins, 160 ESTs (14%) were found to be novel to moth bean. Only 578 ESTs (78%) showed significant BLASTX similarity (pathways. Four hundred and fifty-two ESTs were further annotated with InterProScan (IPS), and no IPS was assigned to 153 ESTs. In addition, the expression level of 27 ESTs in response to heat stress was evaluated through semiquantitative RT-PCR assay. Approximately 20 different signaling genes and 16 different transcription factors have been shown to be associated with heat stress in moth bean for the first time.

  17. Evaluation of selectivity in homologous multimodal chromatographic systems using in silico designed antibody fragment libraries.

    Science.gov (United States)

    Karkov, Hanne Sophie; Woo, James; Krogh, Berit Olsen; Ahmadian, Haleh; Cramer, Steven M

    2015-12-24

    This study describes the in silico design, surface property analyses, production and chromatographic evaluations of a diverse set of antibody Fab fragment variants. Based on previous findings, we hypothesized that the complementarity-determining regions (CDRs) constitute important binding sites for multimodal chromatographic ligands. Given that antibodies are highly diversified molecules and in particular the CDRs, we set out to examine the generality of this result. For this purpose, four different Fab fragments with different CDRs and/or framework regions of the variable domains were identified and related variants were designed in silico. The four Fab variant libraries were subsequently generated by site-directed mutagenesis and produced by recombinant expression and affinity purification to enable examination of their chromatographic retention behavior. The effects of geometric re-arrangement of the functional moieties on the multimodal resin ligands were also investigated with respect to Fab variant retention profiles by comparing two commercially available multimodal cation-exchange ligands, Capto MMC and Nuvia cPrime, and two novel multimodal ligand prototypes. Interestingly, the chromatographic data demonstrated distinct selectivity trends between the four Fab variant libraries. For three of the Fab libraries, the CDR regions appeared as major binding sites for all multimodal ligands. In contrast, the fourth Fab library displayed a distinctly different chromatographic behavior, where Nuvia cPrime and related multimodal ligand prototypes provided markedly improved selectivity over Capto MMC. Clearly, the results illustrate that the discriminating power of multimodal ligands differs between different Fab fragments. The results are promising indications that multimodal chromatography using the appropriate multimodal ligands can be employed in downstream bioprocessing for challenging selective separation of product related variants. Copyright © 2015 Elsevier B

  18. PLOTLIB: a computerized nuclear waste source-term library storage and retrieval system

    International Nuclear Information System (INIS)

    Marshall, J.R.; Nowicki, J.A.

    1978-01-01

    The PLOTLIB code was written to provide computer access to the Nuclear Waste Source-Term Library for those users with little previous computer programming experience. The principles of user orientation, quick accessibility, and versatility were extensively employed in the development of the PLOTLIB code to accomplish this goal. The Nuclear Waste Source-Term Library consists of 16 ORIGEN computer runs incorporating a wide variety of differing light water reactor (LWR) fuel cycles and waste streams. The typical isotopic source-term data consist of information on watts, curies, grams, etc., all of which are compiled as a function of time after reactor discharge and unitized on a per metric ton heavy metal basis. The information retrieval code, PLOTLIB, is used to process source-term information requests into computer plots and/or user-specified output tables. This report will serve both as documentation of the current data library and as an operations manual for the PLOTLIB computer code. The accompanying input description, program listing, and sample problems make this code package an easily understood tool for the various nuclear waste studies under way at the Office of Waste Isolation

  19. WWER440 few group data library preparation and its application in MOBY-DICK modular system

    International Nuclear Information System (INIS)

    Krysl, V.; Mikolas, P.; Svarny, J.

    2002-01-01

    Paper provides summary of methodology of few-group library preparation with emphasis on new features of assemblies, like fuel assemblies with Gd burnable poison or Control Fuel Assembly with Hf plates. Special attention is devoted to the transient part (coupler) of Control Fuel Assembly from the point of view of boundary conditions preparation. Based of this methodology prepared library is implemented into macro code for different number of axial meshes for both coarse and fine mesh diffusion calculations. Problems with local power peaking calculations in WWER-440 cores are closely connected with the correct modeling of the power perturbations in the neighbourhood of Control Fuel Assembly coupler. The new version of MOBY-DICK provided with the new few group data library can assess most of effects induced by insertion of Control Fuel Assembly in the core including effects of newly designed Hf plates in the coupler of Control Fuel Assembly. Compatibility of transport and diffusion calculation was taken into account in the analysis of Control Fuel Assembly movement without/with Hf plates (Authors)

  20. About the Library - Betty Petersen Memorial Library

    Science.gov (United States)

    branch library of the NOAA Central Library. The library serves the NOAA Science Center in Camp Springs , Maryland. History and Mission: Betty Petersen Memorial Library began as a reading room in the NOAA Science Science Center staff and advises the library on all aspects of the library program. Library Newsletters

  1. Library construction and evaluation for site saturation mutagenesis.

    Science.gov (United States)

    Sullivan, Bradford; Walton, Adam Z; Stewart, Jon D

    2013-06-10

    We developed a method for creating and evaluating site-saturation libraries that consistently yields an average of 27.4±3.0 codons of the 32 possible within a pool of 95 transformants. This was verified by sequencing 95 members from 11 independent libraries within the gene encoding alkene reductase OYE 2.6 from Pichia stipitis. Correct PCR primer design as well as a variety of factors that increase transformation efficiency were critical contributors to the method's overall success. We also developed a quantitative analysis of library quality (Q-values) that defines library degeneracy. Q-values can be calculated from standard fluorescence sequencing data (capillary electropherograms) and the degeneracy predicted from an early stage of library construction (pooled plasmids from the initial transformation) closely matched that observed after ca. 1000 library members were sequenced. Based on this experience, we suggest that this analysis can be a useful guide when applying our optimized protocol to new systems, allowing one to focus only on good-quality libraries and reject substandard libraries at an early stage. This advantage is particularly important when lower-throughput screening techniques such as chiral-phase GC must be employed to identify protein variants with desirable properties, e.g., altered stereoselectivities or when multiple codons are targeted for simultaneous randomization. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea

    Science.gov (United States)

    2011-01-01

    Background Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment samples were collected in the depth South China Sea sediments assemblage in plasmid vector containing about 194 Mb of community DNA was prepared. Screening of a part of the unamplified library resulted in isolation of 15 unique lipolytic clones with the ability to hydrolyze tributyrin. A positive recombinant clone (pNLE1), containing a novel esterase (Est_p1), was successfully expressed in E. coli and purified. In a series of assays, Est_p1 displayed maximal activity at pH 8.57, 40°C, with ρ-Nitrophenyl butyrate (C4) as substrate. Compared to other metagenomic esterases, Est_p1 played a notable role in specificity for substrate C4 (kcat/Km value 11,500 S-1m M-1) and showed no inhibited by phenylmethylsulfonyl fluoride, suggested that the substrate binding pocket was suitable for substrate C4 and the serine active-site residue was buried at the bottom of substrate binding pocket which sheltered by a lid structure. Conclusions Esterase, which specificity towards short chain fatty acids, especially butanoic acid, is commercially available as potent flavoring tools. According the outstanding activity and specificity for substrate C4, Est_p1 has potential application in flavor industries requiring hydrolysis of short chain esters. PMID:22067554

  3. A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform.

    Science.gov (United States)

    de Muinck, Eric J; Trosvik, Pål; Gilfillan, Gregor D; Hov, Johannes R; Sundaram, Arvind Y M

    2017-07-06

    Advances in sequencing technologies and bioinformatics have made the analysis of microbial communities almost routine. Nonetheless, the need remains to improve on the techniques used for gathering such data, including increasing throughput while lowering cost and benchmarking the techniques so that potential sources of bias can be better characterized. We present a triple-index amplicon sequencing strategy to sequence large numbers of samples at significantly lower c ost and in a shorter timeframe compared to existing methods. The design employs a two-stage PCR protocol, incorpo rating three barcodes to each sample, with the possibility to add a fourth-index. It also includes heterogeneity spacers to overcome low complexity issues faced when sequencing amplicons on Illumina platforms. The library preparation method was extensively benchmarked through analysis of a mock community in order to assess biases introduced by sample indexing, number of PCR cycles, and template concentration. We further evaluated the method through re-sequencing of a standardized environmental sample. Finally, we evaluated our protocol on a set of fecal samples from a small cohort of healthy adults, demonstrating good performance in a realistic experimental setting. Between-sample variation was mainly related to batch effects, such as DNA extraction, while sample indexing was also a significant source of bias. PCR cycle number strongly influenced chimera formation and affected relative abundance estimates of species with high GC content. Libraries were sequenced using the Illumina HiSeq and MiSeq platforms to demonstrate that this protocol is highly scalable to sequence thousands of samples at a very low cost. Here, we provide the most comprehensive study of performance and bias inherent to a 16S rRNA gene amplicon sequencing method to date. Triple-indexing greatly reduces the number of long custom DNA oligos required for library preparation, while the inclusion of variable length

  4. Library Use

    DEFF Research Database (Denmark)

    Konzack, Lars

    2012-01-01

    A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model.......A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model....

  5. The Effectiveness of Library Instruction: Do Student Response Systems (Clickers Enhance Learning?

    Directory of Open Access Journals (Sweden)

    Katherine McGuire

    2010-06-01

    Full Text Available In the present study, we were interested in determining if library instruction would be more effective if personal response systems (clickers were used during instruction. Furthermore we were interested in examining if students in a class could benefit from clicker technology even if they did not have access to a personal clicker. To examine these issues, we conducted 3 library instruction sessions: Session 1-half of the students were randomly assigned a clicker; Session 2-all students had individual clickers; and Session 3-clickers were not used. Although half of the students in Session 1 did not have clickers, they were presented with all of the information, were aware of the clicker questions, and were presented with the graphs of responses. Students in all 3 sessions completed a pretest and posttest and difference scores were calculated such that positive numbers indicated higher scores. Overall, scores were significantly higher for students who had access to clickers. A comparison of specific clicker use showed that both the individual and group clicker sessions led to significantly higher difference scores. Results indicated that the benefits of clickers are not limited to individual access and group clicker use was as effective. Overall, these results confirm research supporting the integration of technology into classroom instruction.Dans cette étude, nous avons cherché à déterminer si la formation en recherche documentaire était plus efficace lorsqu’on utilisait des systèmes de réponse personnelle (télévoteur. De plus, nous voulions savoir si les étudiants en classe profiteraient de cette technologie même s’ils n’avaient pas accès à un télévoteur individuel. Pour ce faire, nous avons organisé trois séances de formation en recherche documentaire. Pendant la première, nous avons distribué un télévoteur à la moitié des étudiants choisis au hasard. Pendant la deuxième séance, chaque étudiant disposait d

  6. Factors influencing academic library users' intention to use mobile systems:A comparison of current users and potential adopters

    Institute of Scientific and Technical Information of China (English)

    Haijuan YANG; Sisi GUI

    2014-01-01

    Purpose:The study intends to examine the factors influencing the behavioral intention to use academic libraries' mobile systems from the perspective of current users and potential adopters,respectively.Design/methodology/approach:Our study investigates the mobile library system's acceptance by using a context-specific extension of the theory of reasoned action (TRA) and the technology acceptance model (TAM),which includes such factors as mobile self-efficacy,personal innovativeness and perceived playfulness.Structural equation modeling was used to test the validity of the proposed model based on the empirical data which was collected from 210 questionnaire survey participants.Findings:The result shows that 1) for both current users and potential adopters,attitude toward use and subjective norm both have a significant and positive impact on behavioral intention to use;2) perceived usefulness and perceived ease of use are significantly correlated to potential adopters' attitude toward use whereas perceived usefulness and perceived playfulness are significantly related to current users' attitude toward use;3) as for the comparison between the two groups of users,personal innovativeness not only affects perceived usefulness of both current users and potential adopters,but also affects potential adopters' perceived playfulness positively.Mobile self-efficacy has a significant effect on perceived ease of use for both types of users.Research limitations:Although the sample size met the basic statistics requirements for the social research,the participants were mainly college students,and other mobile system users like faculty members and researchers were not investigated.In addition,some influencing factors,such as information quality,system quality and service quality were not considered in the research model.Practical implications:This study reveals main factors which influence both current users and potential adopters' intention to use the mobile system

  7. Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper

    Directory of Open Access Journals (Sweden)

    Dong Ying

    2005-10-01

    Full Text Available Abstract Background Grasshopper serves as important model system in neuroscience, development and evolution. Representatives of this primitive insect group are also highly relevant targets of pest control efforts. Unfortunately, the lack of genetics or gene specific molecular manipulation imposes major limitations to the study of grasshopper biology. Results We investigated whether juvenile instars of the grasshopper species Schistocerca americana are conducive to gene silencing via the systemic RNAi pathway. Injection of dsRNA corresponding to the eye colour gene vermilion into first instar nymphs triggered suppression of ommochrome formation in the eye lasting through two instars equivalent to 10–14 days in absolute time. QRT-PCR analysis revealed a two fold decrease of target transcript levels in affected animals. Control injections of EGFP dsRNA did not result in detectable phenotypic changes. RT-PCR and in situ hybridization detected ubiquitous expression of the grasshopper homolog of the dsRNA channel protein gene sid-1 in embryos, nymphs and adults. Conclusion Our results demonstrate that systemic dsRNA application elicits specific and long-term gene silencing in juvenile grasshopper instars. The conservation of systemic RNAi in the grasshopper suggests that this pathway can be exploited for gene specific manipulation of juvenile and adult instars in a wide range of primitive insects.

  8. A Recent Comparison on Public', Libraries

    Directory of Open Access Journals (Sweden)

    Bülent Yılmaz

    1999-03-01

    Full Text Available Public libraries have been changing by depend on the social development. This changing can create the new problems for public libraries. To make some evaluations on public libraries is important in bringing light to these problems. To compare the public library system and services in a country with the others can give useful results. In these research, Turkish public library system and services has been compared with the public library system dnd services in England, Netherland and Austria. As a result, it hçıs been understood that Turkish public libraries have some important insufficiencies according to the other countries.

  9. The TENDL neutron data library and the TEND1038 38-group neutron constant system

    International Nuclear Information System (INIS)

    Abramovich, S.N.; Gorelov, V.P.; Gorshikhin, A.A.; Grebennikov, A.N.; Il'in, V.N.; Krut'ko, N.A.; Farafontov, G.G.

    2002-01-01

    The library contains neutron data for 103 nuclei - i.e. for 38 actinide nuclei (from 232 Th to 249 Cm), 26 fission fragment nuclei and 39 nuclei in structural and technological materials. The 38-group constants were obtained from TENDL. The high-energy group boundary is 20 MeV. The energy range below 1.2 eV contains 11 groups. Temperature and resonance effects were taken into account. The delayed neutron parameters for 6 groups and the yields of 40 fission fragments were obtained (light and heavy, stable and non-stable). The fast neutron features of spherical critical assemblies were calculated using constants from TEND1038. (author)

  10. A Javascript library that uses Windows Script Host (WSH) to analyze prostate motion data fragmented across a multitude of Excel files by the Calypso 4D Localization System.

    Science.gov (United States)

    Vali, Faisal S; Hsi, Alex; Cho, Paul; Parsai, Homayon; Garver, Elizabeth; Garza, Richard

    2008-11-06

    The Calypso 4D Localization System records prostate motion continuously during radiation treatment. It stores the data across thousands of Excel files. We developed Javascript (JScript) libraries for Windows Script Host (WSH) that use ActiveX Data Objects, OLE Automation and SQL to statistically analyze the data and display the results as a comprehensible Excel table. We then leveraged these libraries in other research to perform vector math on data spread across multiple access databases.

  11. DRIC Library Bulletin Number 3.

    Science.gov (United States)

    TEXTBOOKS, *PERIODICALS, *UNITED KINGDOM, * LIBRARIES , INDEXES, BEHAVIOR, SOCIOLOGY, MEDICINE, CHEMISTRY, OCEANOGRAPHY, ELECTRONICS, MATHEMATICS, ENGINEERING, PHYSICS, COMMUNICATION AND RADIO SYSTEMS, NAVIGATION, DETECTION.

  12. Phylogenetic and functional diversity of metagenomic libraries of phenol degrading sludge from petroleum refinery wastewater treatment system.

    Science.gov (United States)

    Silva, Cynthia C; Hayden, Helen; Sawbridge, Tim; Mele, Pauline; Kruger, Ricardo H; Rodrigues, Marili Vn; Costa, Gustavo Gl; Vidal, Ramon O; Sousa, Maíra P; Torres, Ana Paula R; Santiago, Vânia Mj; Oliveira, Valéria M

    2012-03-27

    In petrochemical refinery wastewater treatment plants (WWTP), different concentrations of pollutant compounds are received daily in the influent stream, including significant amounts of phenolic compounds, creating propitious conditions for the development of particular microorganisms that can rapidly adapt to such environment. In the present work, the microbial sludge from a refinery WWTP was enriched for phenol, cloned into fosmid vectors and pyrosequenced. The fosmid libraries yielded 13,200 clones and a comprehensive bioinformatic analysis of the sequence data set revealed a complex and diverse bacterial community in the phenol degrading sludge. The phylogenetic analyses using MEGAN in combination with RDP classifier showed a massive predominance of Proteobacteria, represented mostly by the genera Diaphorobacter, Pseudomonas, Thauera and Comamonas. The functional classification of phenol degrading sludge sequence data set generated by MG-RAST showed the wide metabolic diversity of the microbial sludge, with a high percentage of genes involved in the aerobic and anaerobic degradation of phenol and derivatives. In addition, genes related to the metabolism of many other organic and xenobiotic compounds, such as toluene, biphenyl, naphthalene and benzoate, were found. Results gathered herein demonstrated that the phenol degrading sludge has complex phylogenetic and functional diversities, showing the potential of such community to degrade several pollutant compounds. This microbiota is likely to represent a rich resource of versatile and unknown enzymes which may be exploited for biotechnological processes such as bioremediation.

  13. Delivering Library Services to Remote Students.

    Science.gov (United States)

    Casado, Margaret

    2001-01-01

    Discusses library services at the University of Tennessee to reach off-campus and distance education students. Topics include online research; email; library instruction for faculty and students; Web interfaces; fax; telephone service; chat technology; the library's Web page; virtual classrooms; library links from a course management system; and…

  14. Engineered CRISPR Systems for Next Generation Gene Therapies.

    Science.gov (United States)

    Pineda, Michael; Moghadam, Farzaneh; Ebrahimkhani, Mo R; Kiani, Samira

    2017-09-15

    An ideal in vivo gene therapy platform provides safe, reprogrammable, and precise strategies which modulate cell and tissue gene regulatory networks with a high temporal and spatial resolution. Clustered regularly interspaced short palindromic repeats (CRISPR), a bacterial adoptive immune system, and its CRISPR-associated protein 9 (Cas9), have gained attention for the ability to target and modify DNA sequences on demand with unprecedented flexibility and precision. The precision and programmability of Cas9 is derived from its complexation with a guide-RNA (gRNA) that is complementary to a desired genomic sequence. CRISPR systems open-up widespread applications including genetic disease modeling, functional screens, and synthetic gene regulation. The plausibility of in vivo genetic engineering using CRISPR has garnered significant traction as a next generation in vivo therapeutic. However, there are hurdles that need to be addressed before CRISPR-based strategies are fully implemented. Some key issues center on the controllability of the CRISPR platform, including minimizing genomic-off target effects and maximizing in vivo gene editing efficiency, in vivo cellular delivery, and spatial-temporal regulation. The modifiable components of CRISPR systems: Cas9 protein, gRNA, delivery platform, and the form of CRISPR system delivered (DNA, RNA, or ribonucleoprotein) have recently been engineered independently to design a better genome engineering toolbox. This review focuses on evaluating CRISPR potential as a next generation in vivo gene therapy platform and discusses bioengineering advancements that can address challenges associated with clinical translation of this emerging technology.

  15. Advances in detection systems of gene and chromosome abnormalities

    International Nuclear Information System (INIS)

    Yatagai, Takeo

    2002-01-01

    This review is described from the aspect of radiation biology. For analysis at gene level, oxidative lesion of DNA like 7,8-dihydro-8-oxoguanine formation and its repair by DNA polymerase η etc in bacteria, yeast and mammalian cells are suggested to be a useful index of radiation mutation. Transgenic mice with E. coli and/or phage gene as a reporter can be a tool for gene analysis for specific organ mutation: data obtained by irradiation of X-ray, γ-ray and accelerated carbon beam to the mouse gpt delta are presented. For analysis from gene to chromosome levels, loss of heterozygosity of a specific gene is a key for analysis of chromosome aberration at the molecular level. Studies in yeast and mammalian cells are presented. The author also described data of gene mutation in TK6 cells irradiated by 2 Gy of X-ray and 10 cGy of carbon beam (135 MeV/u) generated by ring-cycrotron. Human-hamster hybrid cell is an alternative tool. Concerning significance at the individual level, the author quoted studies of irradiation of parent mice resulting in increased incidence of somatic cell mutation and of cancer in offspring. Future systems for gene mutation will be a use of transgenic mice or of markers like a specific cancer. (K.H.)

  16. Monte Carlo simulations of the pulsed thermal neutron flux in two-region hydrogenous systems (using standard MCNP data libraries)

    International Nuclear Information System (INIS)

    Wiacek, U.; Krynicka, E.

    2005-02-01

    Monte Carlo simulations of the pulsed neutron experiment in two- region systems (two concentric spheres and two coaxial finite cylinders) are presented. The MCNP code is used. Aqueous solutions of H 3 BO 3 or KCl are used in the inner region. The outer region is the moderator of Plexiglas. Standard data libraries of the thermal neutron scattering cross-sections of hydrogen in hydrogenous substances are used. The time-dependent thermal neutron transport is simulated when the inner region has a constant size and the external size of the surrounding outer region is variable. The time decay constant of the thermal neutron flux in the system is found in each simulation. The results of the simulations are compared with results of real pulsed neutron experiments on the corresponding systems. (author)

  17. Influence of menstruation on the microbiota of healthy women's labia minora as analyzed using a 16S rRNA gene-based clone library method.

    Science.gov (United States)

    Shiraishi, Tsukasa; Fukuda, Kazumasa; Morotomi, Nobuo; Imamura, Yuri; Mishima, Junko; Imai, Shigeo; Miyazawa, Kiyoshi; Taniguchi, Hatsumi

    2011-01-01

    The aim of this study was to determine the influence of menstruation on the bacterial population of healthy Japanese women's vulvas, especially the labia minora. Labia minora swabs were obtained from 10 premenopausal, nonpregnant Japanese women at premenstruation and on day 2 of menstruation. Vaginal swabs were also obtained from 3 out of the 10 women. No significant difference was found in the average bacterial cell count between the menstruation and premenstruation samples. Molecular analysis using a 16S rRNA gene-based clone library method detected 22 genera from the labia minora swabs (total 20), with the genus Lactobacillus being predominant at both premenstruation and during menstruation in 7 out of the 10 women. Of the other 3 women, 2 showed various kinds of bacterial species, including oral and fecal bacteria, with Atopobium vaginae and Gardnerella vaginalis predominating in the remaining woman's vulva in both conditions. In total, 6 out of 10 cases (60%) showed significantly different microbiota of the labia minora between the two conditions. These results imply that menstruation may promote a distortion of the bacterial flora around the vulva, although it causes no significant increase of the bacterial count.

  18. AMPX: a modular code system for generating coupled multigroup neutron-gamma libraries from ENDF/B

    Energy Technology Data Exchange (ETDEWEB)

    Greene, N.M.; Lucius, J.L.; Petrie, L.M.; Ford, W.E. III; White, J.E.; Wright, R.Q.

    1976-03-01

    AMPX is a modular system for producing coupled multigroup neutron-gamma cross section sets. Basic neutron and gamma cross-section data for AMPX are obtained from ENDF/B libraries. Most commonly used operations required to generate and collapse multigroup cross-section sets are provided in the system. AMPX is flexibly dimensioned; neutron group structures, and gamma group structures, and expansion orders to represent anisotropic processes are all arbitrary and limited only by available computer core and budget. The basic processes provided will (1) generate multigroup neutron cross sections; (2) generate multigroup gamma cross sections; (3) generate gamma yields for gamma-producing neutron interactions; (4) combine neutron cross sections, gamma cross sections, and gamma yields into final ''coupled sets''; (5) perform one-dimensional discrete ordinates transport or diffusion theory calculations for neutrons and gammas and, on option, collapse the cross sections to a broad-group structure, using the one-dimensional results as weighting functions; (6) plot cross sections, on option, to facilitate the ''evaluation'' of a particular multigroup set of data; (7) update and maintain multigroup cross section libraries in such a manner as to make it not only easy to combine new data with previously processed data but also to do it in a single pass on the computer; and (8) output multigroup cross sections in convenient formats for other codes. (auth)

  19. Nigerian Libraries

    African Journals Online (AJOL)

    Bridging the digital divide: the potential role of the National Library of Nigeria · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Juliana Obiageri Akidi, Joy Chituru Onyenachi, 11-19 ...

  20. Library Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Carnegie Library of Pittsburgh locations including address, coordinates, phone number, square footage, and standard operating hours. The map below does not display...

  1. academic libraries

    African Journals Online (AJOL)

    Information Impact: Journal of Information and Knowledge Management

    Information Impact: Journal of Information and Knowledge Management ... Key words: academic libraries, open access, research, researchers, technology ... European commission (2012) reports that affordable and easy access to the results ...

  2. Pancreatic Cancer Gene Therapy: From Molecular Targets to Delivery Systems

    Energy Technology Data Exchange (ETDEWEB)

    Fillat, Cristina, E-mail: cristina.fillat@crg.es; Jose, Anabel; Ros, Xavier Bofill-De; Mato-Berciano, Ana; Maliandi, Maria Victoria; Sobrevals, Luciano [Programa Gens i Malaltia, Centre de Regulació Genòmica-CRG, UPF, Parc de Recerca Biomedica de Barcelona-PRBB and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona (Spain)

    2011-01-18

    The continuous identification of molecular changes deregulating critical pathways in pancreatic tumor cells provides us with a large number of novel candidates to engineer gene-targeted approaches for pancreatic cancer treatment. Targets—both protein coding and non-coding—are being exploited in gene therapy to influence the deregulated pathways to facilitate cytotoxicity, enhance the immune response or sensitize to current treatments. Delivery vehicles based on viral or non-viral systems as well as cellular vectors with tumor homing characteristics are a critical part of the design of gene therapy strategies. The different behavior of tumoral versus non-tumoral cells inspires vector engineering with the generation of tumor selective products that can prevent potential toxic-associated effects. In the current review, a detailed analysis of the different targets, the delivery vectors, the preclinical approaches and a descriptive update on the conducted clinical trials are presented. Moreover, future possibilities in pancreatic cancer treatment by gene therapy strategies are discussed.

  3. Hox gene regulation in the central nervous system of Drosophila

    Directory of Open Access Journals (Sweden)

    Maheshwar eGummalla

    2014-04-01

    Full Text Available Hox genes specify the structures that form along the anteroposterior (AP axis of bilateria. Within the genome, they often form clusters where, remarkably enough, their position within the clusters reflects the relative positions of the structures they specify along the AP axis. This correspondence between genomic organization and gene expression pattern has been conserved through evolution and provides a unique opportunity to study how chromosomal context affects gene regulation. In Drosophila, a general rule, often called posterior dominance, states that Hox genes specifying more posterior structures repress the expression of more anterior Hox genes. This rule explains the apparent spatial complementarity of Hox gene expression patterns in Drosophila. Here we review a noticeable exception to this rule where the more-posteriorly expressed Abd-B hox gene fails to repress the more-anterior abd-A gene in cells of the central nervous system (CNS. While Abd-B is required to repress ectopic expression of abd-A in the posterior epidermis, abd-A repression in the posterior CNS is accomplished by a different mechanism that involves a large 92kb long non-coding RNA (lncRNA encoded by the intergenic region separating abd-A and Abd-B (the iab8ncRNA. Dissection of this lncRNA revealed that abd-A is repressed by the lncRNA using two redundant mechanisms. The 1st mechanism is mediated by a microRNA (mir-iab-8 encoded by intronic sequence within the large iab8-ncRNA. Meanwhile, the second mechanism seems to involve transcriptional interference by the long iab-8 ncRNA on the abd-A promoter. Recent work demonstrating CNS-specific regulation of genes by ncRNAs in Drosophila, seem to highlight a potential role for the iab-8-ncRNA in the evolution of the Drosophila hox complexes

  4. Clinical and academic use of electronic and print books: the Health Sciences Library System e-book study at the University of Pittsburgh.

    Science.gov (United States)

    Folb, Barbara L; Wessel, Charles B; Czechowski, Leslie J

    2011-07-01

    The purpose of the Health Sciences Library System (HSLS) electronic book (e-book) study was to assess use, and factors affecting use, of e-books by all patron groups of an academic health sciences library serving both university and health system-affiliated patrons. A web-based survey was distributed to a random sample (n=5,292) of holders of library remote access passwords. A total of 871 completed and 108 partially completed surveys were received, for an approximate response rate of 16.5%-18.5%, with all user groups represented. Descriptive and chi-square analysis was done using SPSS 17. Library e-books were used by 55.4% of respondents. Use by role varied: 21.3% of faculty reported having assigned all or part of an e-book for class readings, while 86% of interns, residents, and fellows reported using an e-book to support clinical care. Respondents preferred print for textbooks and manuals and electronic format for research protocols, pharmaceutical, and reference books, but indicated high flexibility about format choice. They rated printing and saving e-book content as more important than annotation, highlighting, and bookmarking features. Respondents' willingness to use alternate formats, if convenient, suggests that libraries can selectively reduce title duplication between print and e-books and still support library user information needs, especially if publishers provide features that users want. Marketing and user education may increase use of e-book collections.

  5. 21 CFR 866.5900 - Cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation detection system.

    Science.gov (United States)

    2010-04-01

    ... regulator (CFTR) gene mutation detection system. 866.5900 Section 866.5900 Food and Drugs FOOD AND DRUG...) gene mutation detection system. (a) Identification. The CFTR gene mutation detection system is a device... Guidance Document: CFTR Gene Mutation Detection System.” See § 866.1(e) for the availability of this...

  6. Controlling hospital library theft.

    Science.gov (United States)

    Cuddy, Theresa M; Marchok, Catherine

    2003-04-01

    At Capital Health System/Fuld Campus (formerly Helene Fuld Medical Center), the Health Sciences Library lost many books and videocassettes. These materials were listed in the catalog but were missing when staff went to the shelves. The hospital had experienced a downsizing of staff, a reorganization, and a merger. When the library staff did an inventory, $10,000 worth of materials were found to be missing. We corrected the situation through a series of steps that we believe will help other libraries control their theft. Through regularly scheduling inventories, monitoring items, advertising, and using specific security measures, we have successfully controlled the library theft. The January 2002 inventory resulted in meeting our goal of zero missing books and videocassettes. We work to maintain that goal.

  7. Library activities in the U.S. and the Western Europe : Trends and features in the recent information management system

    Science.gov (United States)

    Takayama, Masaya

    This author reports the discussion and resolutions from the annual meeting of the Special Libraries Association at New York City in the U.S. and the General Conference of the International Federation of Library Associations and Institutions at Paris in France in 1989. Moreover this author visited American libraries after the S.L.A.meeting, and French and British libraries after the IFLA Conference. Based on these library tours, this author concludes that Japanese librarians and information specialists need to know the needs of the application of new information network technologies to the routine works of information management in Japan.

  8. The Security Research of Digital Library Network

    Science.gov (United States)

    Zhang, Xin; Song, Ding-Li; Yan, Shu

    Digital library is a self-development needs for the modern library to meet the development requirements of the times, changing the way services and so on. digital library from the hardware, technology, management and other aspects to objective analysis of the factors of threats to digital library network security. We should face up the problems of digital library network security: digital library network hardware are "not hard", the technology of digital library is relatively lag, digital library management system is imperfect and other problems; the government should take active measures to ensure that the library funding, to enhance the level of network hardware, to upgrade LAN and prevention technology, to improve network control technology, network monitoring technology; to strengthen safety management concepts, to prefect the safety management system; and to improve the level of security management modernization for digital library.

  9. Analysis of PROTEUS phase II experiments performed using the AARE modular system and JEF-based libraries

    International Nuclear Information System (INIS)

    Pelloni, S.; Stepanek, J.; Vontobel, P.

    1989-01-01

    The capability of the advanced analysis of reactor engineering (AARE) modular code system and JEF-1-based nuclear data libraries to analyze light water high converter reactor (LWHCR) lattices is investigated by calculating the wet and dry cells of the PROTEUS-LWHCR phase II experiment. The results are compared to those obtained using several cell codes. Main features of the AARE code system, such as the self-shielding of resonance cross sections in the whole energy range, the generation of adequate fission source spectra, and the efficiency of the elastic removal correction,are investigated. In particular, it is shown that AARE results for the k ∞ void coefficient agree very well with the experiment, whereas other codes give larger deviations

  10. DevLore - A Firmware Library and Web-Based Configuration Control Tool for Accelerator Systems Under Constant Development

    International Nuclear Information System (INIS)

    Richard Evans; Kevin Jordan; Deborah Gruber; Daniel Sexton

    2005-01-01

    The Free Electron Laser Project at Jefferson Lab is based on a comparatively small accelerator driver. As it's systems continues to grow and evolve, strict configuration control has not been a programmatic goal. Conversely, as the IR-Demo FEL and the 10kW IR FEL have been built and operated, hardware and software changes have been regular part of the machine development process. With relatively small component counts for sub-systems, changes occur without requiring much formal documentation and in-situ alterations are common-place in the name of supporting operations. This paper presents an overview of the web-based software tool called DevLore which was first developed to be a library for embedded programming and then became a tremendously effective tool for tracking all changes made to the machine hardware and software

  11. High Energy Physics Forum for Computational Excellence: Working Group Reports (I. Applications Software II. Software Libraries and Tools III. Systems)

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman [Argonne National Lab. (ANL), Argonne, IL (United States); Roser, Robert [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); LeCompte, Tom [Argonne National Lab. (ANL), Argonne, IL (United States); Marshall, Zach [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Borgland, Anders [SLAC National Accelerator Lab., Menlo Park, CA (United States); Viren, Brett [Brookhaven National Lab. (BNL), Upton, NY (United States); Nugent, Peter [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Asai, Makato [SLAC National Accelerator Lab., Menlo Park, CA (United States); Bauerdick, Lothar [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Finkel, Hal [Argonne National Lab. (ANL), Argonne, IL (United States); Gottlieb, Steve [Indiana Univ., Bloomington, IN (United States); Hoeche, Stefan [SLAC National Accelerator Lab., Menlo Park, CA (United States); Sheldon, Paul [Vanderbilt Univ., Nashville, TN (United States); Vay, Jean-Luc [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Elmer, Peter [Princeton Univ., NJ (United States); Kirby, Michael [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Patton, Simon [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Potekhin, Maxim [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Yanny, Brian [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Calafiura, Paolo [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Dart, Eli [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Gutsche, Oliver [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Izubuchi, Taku [Brookhaven National Lab. (BNL), Upton, NY (United States); Lyon, Adam [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Petravick, Don [Univ. of Illinois, Urbana-Champaign, IL (United States). National Center for Supercomputing Applications (NCSA)

    2015-10-29

    Computing plays an essential role in all aspects of high energy physics. As computational technology evolves rapidly in new directions, and data throughput and volume continue to follow a steep trend-line, it is important for the HEP community to develop an effective response to a series of expected challenges. In order to help shape the desired response, the HEP Forum for Computational Excellence (HEP-FCE) initiated a roadmap planning activity with two key overlapping drivers -- 1) software effectiveness, and 2) infrastructure and expertise advancement. The HEP-FCE formed three working groups, 1) Applications Software, 2) Software Libraries and Tools, and 3) Systems (including systems software), to provide an overview of the current status of HEP computing and to present findings and opportunities for the desired HEP computational roadmap. The final versions of the reports are combined in this document, and are presented along with introductory material.

  12. High Energy Physics Forum for Computational Excellence: Working Group Reports (I. Applications Software II. Software Libraries and Tools III. Systems)

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Roser, Robert [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2015-10-28

    Computing plays an essential role in all aspects of high energy physics. As computational technology evolves rapidly in new directions, and data throughput and volume continue to follow a steep trend-line, it is important for the HEP community to develop an effective response to a series of expected challenges. In order to help shape the desired response, the HEP Forum for Computational Excellence (HEP-FCE) initiated a roadmap planning activity with two key overlapping drivers -- 1) software effectiveness, and 2) infrastructure and expertise advancement. The HEP-FCE formed three working groups, 1) Applications Software, 2) Software Libraries and Tools, and 3) Systems (including systems software), to provide an overview of the current status of HEP computing and to present findings and opportunities for the desired HEP computational roadmap. The final versions of the reports are combined in this document, and are presented along with introductory material.

  13. The spectra program library: A PC based system for gamma-ray spectra analysis and INAA data reduction

    Science.gov (United States)

    Baedecker, P.A.; Grossman, J.N.

    1995-01-01

    A PC based system has been developed for the analysis of gamma-ray spectra and for the complete reduction of data from INAA experiments, including software to average the results from mulitple lines and multiple countings and to produce a final report of analysis. Graphics algorithms may be called for the analysis of complex spectral features, to compare the data from alternate photopeaks and to evaluate detector performance during a given counting cycle. A database of results for control samples can be used to prepare quality control charts to evaluate long term precision and to search for systemic variations in data on reference samples as a function of time. The entire software library can be accessed through a user-friendly menu interface with internal help.

  14. Desktop Access to Full-Text NACA and NASA Reports: Systems Developed by NASA Langley Technical Library

    Science.gov (United States)

    Ambur, Manjula Y.; Adams, David L.; Trinidad, P. Paul

    1997-01-01

    NASA Langley Technical Library has been involved in developing systems for full-text information delivery of NACA/NASA technical reports since 1991. This paper will describe the two prototypes it has developed and the present production system configuration. The prototype systems are a NACA CD-ROM of thirty-three classic paper NACA reports and a network-based Full-text Electronic Reports Documents System (FEDS) constructed from both paper and electronic formats of NACA and NASA reports. The production system is the DigiDoc System (DIGItal Documents) presently being developed based on the experiences gained from the two prototypes. DigiDoc configuration integrates the on-line catalog database World Wide Web interface and PDF technology to provide a powerful and flexible search and retrieval system. It describes in detail significant achievements and lessons learned in terms of data conversion, storage technologies, full-text searching and retrieval, and image databases. The conclusions from the experiences of digitization and full- text access and future plans for DigiDoc system implementation are discussed.

  15. Climate information for public health: the role of the IRI climate data library in an integrated knowledge system.

    Science.gov (United States)

    del Corral, John; Blumenthal, M Benno; Mantilla, Gilma; Ceccato, Pietro; Connor, Stephen J; Thomson, Madeleine C

    2012-09-01

    Public health professionals are increasingly concerned about the potential impact of climate variability and change on health outcomes. Protecting public health from the vagaries of climate requires new working relationships between the public health sector and the providers of climate data and information. The Climate Information for Public Health Action initiative at the International Research Institute for Climate and Society (IRI) is designed to increase the public health community's capacity to understand, use and demand appropriate climate data and climate information to mitigate the public health impacts of the climate. Significant challenges to building the capacity of health professionals to use climate information in research and decision-making include the difficulties experienced by many in accessing relevant and timely quality controlled data and information in formats that can be readily incorporated into specific analysis with other data sources. We present here the capacities of the IRI climate data library and show how we have used it to build an integrated knowledge system in the support of the use of climate and environmental information in climate-sensitive decision-making with respect to health. Initiated as an aid facilitating exploratory data analysis for climate scientists, the IRI climate data library has emerged as a powerful tool for interdisciplinary researchers focused on topics related to climate impacts on society, including health.

  16. Factors influencing academic library users’ intention to use mobile systems: A comparison of current users and potential adopters

    Institute of Scientific and Technical Information of China (English)

    Haijuan; YANG; Sisi; GUI

    2014-01-01

    Purpose: The study intends to examine the factors influencing the behavioral intention to use academic libraries’ mobile systems from the perspective of current users and potential adopters, respectively. Design/methodology/approach: Our study investigates the mobile library system’s acceptance by using a context-specific extension of the theory of reasoned action(TRA) and the technology acceptance model(TAM), which includes such factors as mobile self-efficacy, personal innovativeness and perceived playfulness. Structural equation modeling was used to test the validity of the proposed model based on the empirical data which was collected from 210 questionnaire survey participants.Findings: The result shows that 1) for both current users and potential adopters, attitude toward use and subjective norm both have a significant and positive impact on behavioral intention to use; 2) perceived usefulness and perceived ease of use are significantly correlated to potential adopters’ attitude toward use whereas perceived usefulness and perceived playfulness are significantly related to current users’ attitude toward use; 3) as for the comparison between the two groups of users, personal innovativeness not only affects perceived usefulness of both current users and potential adopters, but also affects potential adopters’ perceived playfulness positively. Mobile self-efficacy has a significant effect on perceived ease of use for both types of users.Research limitations: Although the sample size met the basic statistics requirements for the social research, the participants were mainly college students, and other mobile system users like faculty members and researchers were not investigated. In addition, some influencing factors, such as information quality, system quality and service quality were not considered in the research model.Practical implications: This study reveals main factors which influence both current users and potential adopters’ intention to use

  17. Tetracycline-inducible gene expression system in Leishmania mexicana

    Czech Academy of Sciences Publication Activity Database

    Kraeva, N.; Ishemgulova, A.; Lukeš, Julius; Yurchenko, Vyacheslav

    2014-01-01

    Roč. 198, č. 1 (2014), s. 11-13 ISSN 0166-6851 R&D Projects: GA MŠk(CZ) EE2.3.30.0032 Institutional support: RVO:60077344 Keywords : Leishmania mexicana * Gene expression * Tet-inducible system Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.787, year: 2014

  18. Molecular transformation, gene cloning, and gene expression systems for filamentous fungi

    Science.gov (United States)

    Gold, Scott E.; Duick, John W.; Redman, Regina S.; Rodriguez, Rusty J.

    2001-01-01

    This chapter discusses the molecular transformation, gene cloning, and gene expression systems for filamentous fungi. Molecular transformation involves the movement of discrete amounts of DNA into cells, the expression of genes on the transported DNA, and the sustainable replication of the transforming DNA. The ability to transform fungi is dependent on the stable replication and expression of genes located on the transforming DNA. Three phenomena observed in bacteria, that is, competence, plasmids, and restriction enzymes to facilitate cloning, were responsible for the development of molecular transformation in fungi. Initial transformation success with filamentous fungi, involving the complementation of auxotrophic mutants by exposure to sheared genomic DNA or RNA from wt isolates, occurred with low transformation efficiencies. In addition, it was difficult to retrieve complementing DNA fragments and isolate genes of interest. This prompted the development of transformation vectors and methods to increase efficiencies. The physiological studies performed with fungi indicated that the cell wall could be removed to generate protoplasts. It was evident that protoplasts could be transformed with significantly greater efficiencies than walled cells.

  19. Developing Plugin e-DDC as an Additional Application for Senayan Library Management System with PHP Language Programming and MySQL Database

    Directory of Open Access Journals (Sweden)

    Mohamad Rotmianto

    2018-01-01

    Full Text Available Between Senayan Library Management System or usually called SLiMS and e-DDC (electronic Dewey Decimal Classification now is the most popular library application software which has a lot of user, because it is simple to use, has an updating guarantee from its developers and off course both of them are free of charge. Although SLiMS and e-DDC are different application at all, as practically they are recommended to be used togather for library management. SLiMS is used for library automation and e-DDC is to find collection’s classification. Many users of SLiMS and e-DDC ever give suggestions about developing SLiMS with e-DDC include in its database, and then librarians will be easier to manage their libraries. Because of that suggestion, finally a plugin as an additional application for SLiMS has been created and developed. That plugin was build with PHP language programming and MySQL database. The purpose of this paper is to enrich about reference of development of library application, especially those based on Free Open Source Software (FOSS. This paper use Research and Development Methods. And the result of this paper is Plugin e-DDC for SLiMS which has released on May, 2nd 2015, in order to celebrate “National Education Day”.

  20. Systems Biology-Based Investigation of Cellular Antiviral Drug Targets Identified by Gene-Trap Insertional Mutagenesis.

    Directory of Open Access Journals (Sweden)

    Feixiong Cheng

    2016-09-01

    Full Text Available Viruses require host cellular factors for successful replication. A comprehensive systems-level investigation of the virus-host interactome is critical for understanding the roles of host factors with the end goal of discovering new druggable antiviral targets. Gene-trap insertional mutagenesis is a high-throughput forward genetics approach to randomly disrupt (trap host genes and discover host genes that are essential for viral replication, but not for host cell survival. In this study, we used libraries of randomly mutagenized cells to discover cellular genes that are essential for the replication of 10 distinct cytotoxic mammalian viruses, 1 gram-negative bacterium, and 5 toxins. We herein reported 712 candidate cellular genes, characterizing distinct topological network and evolutionary signatures, and occupying central hubs in the human interactome. Cell cycle phase-specific network analysis showed that host cell cycle programs played critical roles during viral replication (e.g. MYC and TAF4 regulating G0/1 phase. Moreover, the viral perturbation of host cellular networks reflected disease etiology in that host genes (e.g. CTCF, RHOA, and CDKN1B identified were frequently essential and significantly associated with Mendelian and orphan diseases, or somatic mutations in cancer. Computational drug repositioning framework via incorporating drug-gene signatures from the Connectivity Map into the virus-host interactome identified 110 putative druggable antiviral targets and prioritized several existing drugs (e.g. ajmaline that may be potential for antiviral indication (e.g. anti-Ebola. In summary, this work provides a powerful methodology with a tight integration of gene-trap insertional mutagenesis testing and systems biology to identify new antiviral targets and drugs for the development of broadly acting and targeted clinical antiviral therapeutics.

  1. The WIMSLIB library - neutron data library for WIMS-D

    International Nuclear Information System (INIS)

    Liu Ping

    1998-05-01

    During a visit to the IAEA Nuclear Data Section from 13 June to 12 December 1997, the author processed the Chinese Evaluated Nuclear Data Library (CENDL), Version 2.1, using the NJOY Nuclear Data Processing System, Version 94.105, to generate the working library WIMSLIB for input to WIMS-D/4 and WIMS-D/5A. The WIMSLIB library was then used to perform benchmark testing of CENDL-2.1. (author)

  2. Conditions for success of engineered underdominance gene drive systems.

    Science.gov (United States)

    Edgington, Matthew P; Alphey, Luke S

    2017-10-07

    Engineered underdominance is one of a number of different gene drive strategies that have been proposed for the genetic control of insect vectors of disease. Here we model a two-locus engineered underdominance based gene drive system that is based on the concept of mutually suppressing lethals. In such a system two genetic constructs are introduced, each possessing a lethal element and a suppressor of the lethal at the other locus. Specifically, we formulate and analyse a population genetics model of this system to assess when different combinations of release strategies (i.e. single or multiple releases of both sexes or males only) and genetic systems (i.e. bisex lethal or female-specific lethal elements and different strengths of suppressors) will give population replacement or fail to do so. We anticipate that results presented here will inform the future design of engineered underdominance gene drive systems as well as providing a point of reference regarding release strategies for those looking to test such a system. Our discussion is framed in the context of genetic control of insect vectors of disease. One of several serious threats in this context are Aedes aegypti mosquitoes as they are the primary vectors of dengue viruses. However, results are also applicable to Ae. aegypti as vectors of Zika, yellow fever and chikungunya viruses and also to the control of a number of other insect species and thereby of insect-vectored pathogens. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  3. Validation of SCALE 4.0 -- CSAS25 module and the 27-group ENDF/B-IV cross-section library for low-enriched uranium systems

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, W.C.

    1993-02-01

    A version of KENO V.a and the 27-group library in SCALE-4.0 were validated for use in evaluating the nuclear criticality safety of low-enriched uranium systems. A total of 59 critical systems were analyzed. A statistical analysis of the results was performed, and subcritical acceptanced criteria are established.

  4. Validation of SCALE 4. 0 -- CSAS25 module and the 27-group ENDF/B-IV cross-section library for low-enriched uranium systems

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, W.C.

    1993-02-01

    A version of KENO V.a and the 27-group library in SCALE-4.0 were validated for use in evaluating the nuclear criticality safety of low-enriched uranium systems. A total of 59 critical systems were analyzed. A statistical analysis of the results was performed, and subcritical acceptanced criteria are established.

  5. Opening up Library Automation Software

    Science.gov (United States)

    Breeding, Marshall

    2009-01-01

    Throughout the history of library automation, the author has seen a steady advancement toward more open systems. In the early days of library automation, when proprietary systems dominated, the need for standards was paramount since other means of inter-operability and data exchange weren't possible. Today's focus on Application Programming…

  6. How Much Security Does Your Library Need?

    Science.gov (United States)

    Banerjee, Kyle

    2003-01-01

    Explains how to keep library systems healthy and functioning by taking sensible security measures. Examines why hackers would target library systems and how library systems are compromised. Describes tools that can help, including: firewalls; antivirus software; alarms; network analysis tools; and encryption. Identifies several strategies for…

  7. Libraries within the system of the institutions of national memory of Ukraine and national cultural heritage

    Directory of Open Access Journals (Sweden)

    Dubrovina L. A.

    2016-01-01

    Full Text Available In the article, libraries, archives and museums are explored as the constituents of social institutes realizing state policy of social, historical and national memory The institutes organize deposition of memory sources, conduct scientific inquiry and identification, study the origins of documents, museum exhibits and collections, the history of documental heritage. Their main functions are preservation and study of historical and cultural sources, sharing unbiased information and scientifically grounded knowledge concerning the documental cultural heritage and the sources’ use in contemporary information analysis, memorial, socio-political, cultural-educational and other events, aimed at the formation and consolidation of national memory. It is believed, that through the abovementioned actions these social institutions contribute to the informational safety of a country, its integration into the world humanitarian space. The institutions activity provides governmental figures, educators, scientists and cultural figures with unbiased information, conduces countermeasures against the tendentious interpretation of historical process and the deformation of memorial senses of the past and modernity, the manipulation of historical facts and socio-cultural factors sense

  8. Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System

    Directory of Open Access Journals (Sweden)

    Gang Yu

    2017-11-01

    Full Text Available Plant productivity is limited by salinity stress, both in natural and agricultural systems. Identification of salt stress-related genes from halophyte can provide insights into mechanisms of salt stress tolerance in plants. Atriplex canescens is a xero-halophyte that exhibits optimum growth in the presence of 400 mM NaCl. A cDNA library derived from highly salt-treated A. canescens plants was constructed based on a yeast expression system. A total of 53 transgenic yeast clones expressing enhanced salt tolerance were selected from 105 transformants. Their plasmids were sequenced and the gene characteristics were annotated using a BLASTX search. Retransformation of yeast cells with the selected plasmids conferred salt tolerance to the resulting transformants. The expression patterns of 28 of these stress-related genes were further investigated in A. canescens leaves by quantitative reverse transcription-PCR. In this study, we provided a rapid and robust assay system for large-scale screening of genes for varied abiotic stress tolerance with high efficiency in A. canescens.

  9. Screening for proteins interacting with MCM7 in human lung cancer library using yeast two hybrid system

    Directory of Open Access Journals (Sweden)

    Yuchen HAN

    2008-08-01

    Full Text Available Background and objective MCM7 is a subunit of the MCM complex that plays a key role in DNA replication initiation. But little is known about its interaction proteins. In this study yeast two hybrid screening was used to identify the MCM7 interacting proteins. Methods Yeast expression vector containing human full length MCM7-pGBKT7 plasmid was constructed, and with a library of cDNAs from human lung cancer-pACT2 plasmid was transformed into yeast strain AH109, and was electively grew in X-a-gal auxotrophy medium SD/-Trp-Leu-His-Ade, and the blue colonies were picked up, the plasmid of the yeast colonies was extracted , and transformed into E. Coli to extract DNA and performed sequence analysis. Results Eleven proteins were identified which could specifically interact with MCM7 proteins, among these five were cytoskeleton proteins, six were enzymes, kinases and related receptors. Conclusion The investigation provides functional clues for further exploration of MCM7 gene.

  10. Body Basics Library

    Science.gov (United States)

    ... Body Basics articles explain just how each body system, part, and process works. Use this medical library to find out about basic human anatomy, how ... Teeth Skin, Hair, and Nails Spleen and Lymphatic System ... Visit the Nemours Web site. Note: All information on TeensHealth® is for ...

  11. Personal Virtual Libraries

    Science.gov (United States)

    Pappas, Marjorie L.

    2004-01-01

    Virtual libraries are becoming more and more common. Most states have a virtual library. A growing number of public libraries have a virtual presence on the Web. Virtual libraries are a growing addition to school library media collections. The next logical step would be personal virtual libraries. A personal virtual library (PVL) is a collection…

  12. America's Star Libraries

    Science.gov (United States)

    Lyons, Ray; Lance, Keith Curry

    2009-01-01

    "Library Journal"'s new national rating of public libraries, the "LJ" Index of Public Library Service, identifies 256 "star" libraries. It rates 7,115 public libraries. The top libraries in each group get five, four, or three Michelin guide-like stars. All included libraries, stars or not, can use their scores to learn from their peers and improve…

  13. A model library for dynamic transport and fate of micropollutants in integrated urban wastewater and stormwater systems

    DEFF Research Database (Denmark)

    Vezzaro, Luca; Benedetti, Lorenzo; Gevaert, Veerle

    2014-01-01

    by using substance inherent properties, following an approach commonly used in large-scale MP multimedia fate and transport models. The chosen level of complexity ensures a low data requirement and minimizes the need for field measurements. Next to a synthesis of model applications, a didactic example......The increasing efforts in reducing the emission of micropollutants (MP) into the natural aquatic environment require the development of modelling tools to support the decision making process. This article presents a library of dynamic modelling tools for estimating MP fluxes within Integrated Urban...... Wastewater and Stormwater system (IUWS – including drainage network, stormwater treatment units, wastewater treatment plants, sludge treatment, and the receiving water body). The models are developed by considering the high temporal variability of the processes taking place in the IUWS, providing a basis...

  14. From gene networks to drugs: systems pharmacology approaches for AUD.

    Science.gov (United States)

    Ferguson, Laura B; Harris, R Adron; Mayfield, Roy Dayne

    2018-06-01

    The alcohol research field has amassed an impressive number of gene expression datasets spanning key brain areas for addiction, species (humans as well as multiple animal models), and stages in the addiction cycle (binge/intoxication, withdrawal/negative effect, and preoccupation/anticipation). These data have improved our understanding of the molecular adaptations that eventually lead to dysregulation of brain function and the chronic, relapsing disorder of addiction. Identification of new medications to treat alcohol use disorder (AUD) will likely benefit from the integration of genetic, genomic, and behavioral information included in these important datasets. Systems pharmacology considers drug effects as the outcome of the complex network of interactions a drug has rather than a single drug-molecule interaction. Computational strategies based on this principle that integrate gene expression signatures of pharmaceuticals and disease states have shown promise for identifying treatments that ameliorate disease symptoms (called in silico gene mapping or connectivity mapping). In this review, we suggest that gene expression profiling for in silico mapping is critical to improve drug repurposing and discovery for AUD and other psychiatric illnesses. We highlight studies that successfully apply gene mapping computational approaches to identify or repurpose pharmaceutical treatments for psychiatric illnesses. Furthermore, we address important challenges that must be overcome to maximize the potential of these strategies to translate to the clinic and improve healthcare outcomes.

  15. Future of research libraries

    CERN Document Server

    Naryandas, Narakesari; Kindström, Daniel

    2014-01-01

    Research libraries have been an integral part of the scholarly communication system since that system emerged in its present form. They now face a period of unprecedentedly drastic and rapid change. This is caused, first and foremost, by the migration of much scholarly material to digital formats, raising the question of the future purpose of the 'library space'. Together with this come transfigurational changes to the communication change of recorded information, with the roles of authors , publishers, database producers and librarians and archivists all in a state of flux. Finally, new forms

  16. Wordpress for libraries

    CERN Document Server

    Haefele, Chad

    2015-01-01

    WordPress is not only the most popular blogging software in the world, but it is also a powerful content management system that runs more than 23 percent of all websites. The current version alone has been downloaded almost 20 million times, and the WordPress community has built more than 38,000 plugins to extend and enhance the system. Libraries are using this technology to create community-oriented websites, blogs, subject guides, digital archives, and more. This hands-on, practical book walks readers through the entire process of setting up a WordPress website for their library,

  17. In vivo characterization of a reporter gene system for imaging hypoxia-induced gene expression.

    Science.gov (United States)

    Carlin, Sean; Pugachev, Andrei; Sun, Xiaorong; Burke, Sean; Claus, Filip; O'Donoghue, Joseph; Ling, C Clifton; Humm, John L

    2009-10-01

    To characterize a tumor model containing a hypoxia-inducible reporter gene and to demonstrate utility by comparison of reporter gene expression to the uptake and distribution of the hypoxia tracer (18)F-fluoromisonidazole ((18)F-FMISO). Three tumors derived from the rat prostate cancer cell line R3327-AT were grown in each of two rats as follows: (1) parental R3327-AT, (2) positive control R3327-AT/PC in which the HSV1-tkeGFP fusion reporter gene was expressed constitutively, (3) R3327-AT/HRE in which the reporter gene was placed under the control of a hypoxia-inducible factor-responsive promoter sequence (HRE). Animals were coadministered a hypoxia-specific marker (pimonidazole) and the reporter gene probe (124)I-2'-fluoro-2'-deoxy-1-beta-d-arabinofuranosyl-5-iodouracil ((124)I-FIAU) 3 h prior to sacrifice. Statistical analysis of the spatial association between (124)I-FIAU uptake and pimonidazole fluorescent staining intensity was then performed on a pixel-by-pixel basis. Utility of this system was demonstrated by assessment of reporter gene expression versus the exogenous hypoxia probe (18)F-FMISO. Two rats, each bearing a single R3327-AT/HRE tumor, were injected with (124)I-FIAU (3 h before sacrifice) and (18)F-FMISO (2 h before sacrifice). Statistical analysis of the spatial association between (18)F-FMISO and (124)I-FIAU on a pixel-by-pixel basis was performed. Correlation coefficients between (124)I-FIAU uptake and pimonidazole staining intensity were: 0.11 in R3327-AT tumors, -0.66 in R3327-AT/PC and 0.76 in R3327-AT/HRE, confirming that only in the R3327-AT/HRE tumor was HSV1-tkeGFP gene expression associated with hypoxia. Correlation coefficients between (18)F-FMISO and (124)I-FIAU uptakes in R3327-AT/HRE tumors were r=0.56, demonstrating good spatial correspondence between the two tracers. We have confirmed hypoxia-specific expression of the HSV1-tkeGFP fusion gene in the R3327-AT/HRE tumor model and demonstrated the utility of this model for the

  18. In vivo characterization of a reporter gene system for imaging hypoxia-induced gene expression

    International Nuclear Information System (INIS)

    Carlin, Sean; Pugachev, Andrei; Sun Xiaorong; Burke, Sean; Claus, Filip; O'Donoghue, Joseph; Ling, C. Clifton; Humm, John L.

    2009-01-01

    Purpose: To characterize a tumor model containing a hypoxia-inducible reporter gene and to demonstrate utility by comparison of reporter gene expression to the uptake and distribution of the hypoxia tracer 18 F-fluoromisonidazole ( 18 F-FMISO). Methods: Three tumors derived from the rat prostate cancer cell line R3327-AT were grown in each of two rats as follows: (1) parental R3327-AT, (2) positive control R3327-AT/PC in which the HSV1-tkeGFP fusion reporter gene was expressed constitutively, (3) R3327-AT/HRE in which the reporter gene was placed under the control of a hypoxia-inducible factor-responsive promoter sequence (HRE). Animals were coadministered a hypoxia-specific marker (pimonidazole) and the reporter gene probe 124 I-2'-fluoro-2'-deoxy-1-β-D-arabinofuranosyl-5-iodouracil ( 124 I-FIAU) 3 h prior to sacrifice. Statistical analysis of the spatial association between 124 I-FIAU uptake and pimonidazole fluorescent staining intensity was then performed on a pixel-by-pixel basis. Utility of this system was demonstrated by assessment of reporter gene expression versus the exogenous hypoxia probe 18 F-FMISO. Two rats, each bearing a single R3327-AT/HRE tumor, were injected with 124 I-FIAU (3 h before sacrifice) and 18 F-FMISO (2 h before sacrifice). Statistical analysis of the spatial association between 18 F-FMISO and 124 I-FIAU on a pixel-by-pixel basis was performed. Results: Correlation coefficients between 124 I-FIAU uptake and pimonidazole staining intensity were: 0.11 in R3327-AT tumors, -0.66 in R3327-AT/PC and 0.76 in R3327-AT/HRE, confirming that only in the R3327-AT/HRE tumor was HSV1-tkeGFP gene expression associated with hypoxia. Correlation coefficients between 18 F-FMISO and 124 I-FIAU uptakes in R3327-AT/HRE tumors were r=0.56, demonstrating good spatial correspondence between the two tracers. Conclusions: We have confirmed hypoxia-specific expression of the HSV1-tkeGFP fusion gene in the R3327-AT/HRE tumor model and demonstrated the utility of

  19. USH2A Gene Editing Using the CRISPR System

    Directory of Open Access Journals (Sweden)

    Carla Fuster-García

    2017-09-01

    Full Text Available Usher syndrome (USH is a rare autosomal recessive disease and the most common inherited form of combined visual and hearing impairment. Up to 13 genes are associated with this disorder, with USH2A being the most prevalent, due partially to the recurrence rate of the c.2299delG mutation. Excluding hearing aids or cochlear implants for hearing impairment, there are no medical solutions available to treat USH patients. The repair of specific mutations by gene editing is, therefore, an interesting strategy that can be explored using the CRISPR/Cas9 system. In this study, this method of gene editing is used to target the c.2299delG mutation on fibroblasts from an USH patient carrying the mutation in homozygosis. Successful in vitro mutation repair was demonstrated using locus-specific RNA-Cas9 ribonucleoproteins with subsequent homologous recombination repair induced by an engineered template supply. Effects on predicted off-target sites in the CRISPR-treated cells were discarded after a targeted deep-sequencing screen. The proven effectiveness and specificity of these correction tools, applied to the c.2299delG pathogenic variant of USH2A, indicates that the CRISPR system should be considered to further explore a potential treatment of USH. Keywords: Usher syndrome, USH2A, c.2299delG, CRISPR, gene editing, RNPs

  20. Using interpolation to estimate system uncertainty in gene expression experiments.

    Directory of Open Access Journals (Sweden)

    Lee J Falin

    Full Text Available The widespread use of high-throughput experimental assays designed to measure the entire complement of a cell's genes or gene products has led to vast stores of data that are extremely plentiful in terms of the number of items they can measure in a single sample, yet often sparse in the number of samples per experiment due to their high cost. This often leads to datasets where the number of treatment levels or time points sampled is limited, or where there are very small numbers of technical and/or biological replicates. Here we introduce a novel algorithm to quantify the uncertainty in the unmeasured intervals between biological measurements taken across a set of quantitative treatments. The algorithm provides a probabilistic distribution of possible gene expression values within unmeasured intervals, based on a plausible biological constraint. We show how quantification of this uncertainty can be used to guide researchers in further data collection by identifying which samples would likely add the most information to the system under study. Although the context for developing the algorithm was gene expression measurements taken over a time series, the approach can be readily applied to any set of quantitative systems biology measurements taken following quantitative (i.e. non-categorical treatments. In principle, the method could also be applied to combinations of treatments, in which case it could greatly simplify the task of exploring the large combinatorial space of future possible measurements.

  1. Developing Plugin e-DDC as an Additional Application for Senayan Library Management System with PHP Language Programming and MySQL Database

    Directory of Open Access Journals (Sweden)

    Mohamad Rotmianto

    2016-07-01

    Full Text Available Senayan Library Management System, usually called as SLiMS dan e-DDC (electronic Dewey Decimal Classification, is an application for library that now is used widely because it is easy to use, guaranteed of updating from the provider, and free. SLiMs and e-DDC is actually two separated application, but in the practice, it is possible and recommended to be used together. SLiMS is used as a tool of library automation, meanwhile e-DDC functions to help finding collection classification number. Some users of SLiMS and e-DDC have suggested to develop SLiMS that is integral with e-DDC, so the use of both application can be practical which can make the librarian easier to manage the library. Based on the suggestion, the writer and e-DDC team finally created and designed additional application which is called plugin in SLiMS automation system that contained e-DDC. The plugin was created using PHP MySQL and MySQL database. The purpose of this paper is to enrich the reference of library application development, especially that is based on Free Open Source Software (FOSS. The method used is Research and Development method. The result was the Plugin e-DDC for SLiMS was finished and released on May 2, 2015 to celebrate National Education Day.

  2. Slovenian Public Libraries in 2010

    Directory of Open Access Journals (Sweden)

    Gorazd Vodeb

    2013-09-01

    Full Text Available ABSTRACTPurpose:  The article presents the 2010 Slovenian public library statistics. Performance indicators are compared to library regulations and standards, and to the goals of the Resolution on the 2008-2011 National Programme for Culture. Opening time, library space and equipment, the size of collections and the number of additions are surveyed. Staff structure and financial data are presented as well as the data about visitors, registered members, loans and other library services.   Methodology/approach:  Data collected via the annual statistical survey of Slovenian public libraries are used. They were collected and published by the Library System Development Centre at the National & University Library.   Results:  Data show the stability of the public library income. The level of resources has been generally increased. The number of loans and visits are still rising whereas the number of registered users is slightly dropping. The extent of digitalisation of library materials has been significantly increased.       Research limitation:  The average value of performance indicators is presented.      Originality/practical implications:  Results can be used for monitoring public library development.

  3. A data library management system for midwest FreightView and its data repository.

    Science.gov (United States)

    2011-03-01

    Midwest FreightView (MWFV) and its associated data repository is part of a large multifaceted : effort to promote regional economic development throughout the Great Lakes : system. The main objective for the system is to promote sustainable maritime ...

  4. DOLIB: Distributed Object Library

    Energy Technology Data Exchange (ETDEWEB)

    D' Azevedo, E.F.

    1994-01-01

    This report describes the use and implementation of DOLIB (Distributed Object Library), a library of routines that emulates global or virtual shared memory on Intel multiprocessor systems. Access to a distributed global array is through explicit calls to gather and scatter. Advantages of using DOLIB include: dynamic allocation and freeing of huge (gigabyte) distributed arrays, both C and FORTRAN callable interfaces, and the ability to mix shared-memory and message-passing programming models for ease of use and optimal performance. DOLIB is independent of language and compiler extensions and requires no special operating system support. DOLIB also supports automatic caching of read-only data for high performance. The virtual shared memory support provided in DOLIB is well suited for implementing Lagrangian particle tracking techniques. We have also used DOLIB to create DONIO (Distributed Object Network I/O Library), which obtains over a 10-fold improvement in disk I/O performance on the Intel Paragon.

  5. DOLIB: Distributed Object Library

    Energy Technology Data Exchange (ETDEWEB)

    D`Azevedo, E.F.; Romine, C.H.

    1994-10-01

    This report describes the use and implementation of DOLIB (Distributed Object Library), a library of routines that emulates global or virtual shared memory on Intel multiprocessor systems. Access to a distributed global array is through explicit calls to gather and scatter. Advantages of using DOLIB include: dynamic allocation and freeing of huge (gigabyte) distributed arrays, both C and FORTRAN callable interfaces, and the ability to mix shared-memory and message-passing programming models for ease of use and optimal performance. DOLIB is independent of language and compiler extensions and requires no special operating system support. DOLIB also supports automatic caching of read-only data for high performance. The virtual shared memory support provided in DOLIB is well suited for implementing Lagrangian particle tracking techniques. We have also used DOLIB to create DONIO (Distributed Object Network I/O Library), which obtains over a 10-fold improvement in disk I/O performance on the Intel Paragon.

  6. MARKETING LIBRARY SERVICES IN ACADEMIC LIBRARIES: A ...

    African Journals Online (AJOL)

    MARKETING LIBRARY SERVICES IN ACADEMIC LIBRARIES: A TOOL FOR SURVIVAL IN THE ... This article discusses the concept of marketing library and information services as an ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  7. Toward Continual Reform: Progress in Academic Libraries in China.

    Science.gov (United States)

    Ping, Ke

    2002-01-01

    Traces developments in China's academic libraries: managing human resources, restructuring library developments, revising and implementing new policies, evaluating services and operations, establishing library systems, building new structures, and exploring joint-use library models. Major focus was to improve services for library user. (Author/LRW)

  8. Vascular targeting with peptide libraries

    Energy Technology Data Exchange (ETDEWEB)

    Pasqualini, R. [La Jolla Cancer Research Center The Burnham Inst., La Jolla CA (United States)

    1999-06-01

    The authors have developed an 'in vivo' selection system in which phage capable of selective homing to different tissues are recovered from a phage display peptide library following intravenous administration. Using this strategy, they have isolate several organ and tumor-homing peptides. They have shown that each of those peptides binds of different receptors that are selectively expressed on the vasculature of the target tissue. The tumor-homing peptides bind to receptors that are up regulated in tumor angiogenic vasculature. Targeted delivery of doxorubicin to angiogenic vasculature using these peptides in animals models decrease toxicity and increased the therapeutic efficacy of the drug. Vascular targeting may facilitate the development of other treatment strategies that rely on inhibition of angio genesis and lead to advances to extend the potential for targeting of drugs, genes and radionuclides in the context of many diseases.

  9. Perinatal systemic gene delivery using adeno-associated viral vectors

    Directory of Open Access Journals (Sweden)

    Rajvinder eKarda

    2014-11-01

    Full Text Available Neurodegenerative monogenic diseases can also affect a broad range of tissues and organs throughout the body. An effective treatment would require a systemic approach. The intravenous administration of novel therapies is ideal but is hampered by the inability of such drugs to cross the blood-brain barrier and precludes efficacy in the central nervous system. A number of these early lethal intractable diseases also present devastating irreversible pathology at birth or soon after. Therefore, any therapy would ideally be administered during the perinatal period to prevent, stop or ameliorate disease progression. The concept of perinatal gene therapy has moved a step further towards being a feasible approach to treating such disorders. This has primarily been driven by the recent discoveries that particular serotypes of adeno-associated virus (AAV gene delivery vectors have the ability to cross the blood-brain barrier following intravenous administration. Furthermore, this has been safely demonstrated in perinatal mice and non-human primates. This review focuses on the progress made in using AAV to achieve systemic transduction and what this means for developing perinatal gene therapy for early lethal neurodegenerative diseases.

  10. Digital library research : current developments and trends

    OpenAIRE

    Shiri, Ali

    2003-01-01

    This column gives an overview of current trends in digital library research under the following headings: digital library architecture, systems, tools and technologies; digital content and collections; metadata; interoperability; standards; knowledge organisation systems; users and usability; legal, organisational, economic, and social issues in digital libraries.

  11. Identity of a brand new library

    KAUST Repository

    Vijayakumar, J.K.

    2017-01-01

    King Abdullah University of Science and Technology (KAUST) Library in Saudi Arabia aspires to be one of the great new-generation libraries in the field of scientific research. Its services and systems support a digitally born collection, 97

  12. An Intelligent Mobile Location-Aware Book Recommendation System that Enhances Problem-Based Learning in Libraries

    Science.gov (United States)

    Chen, Chih-Ming

    2013-01-01

    Despite rapid and continued adoption of mobile devices, few learning modes integrate with mobile technologies and libraries' environments as innovative learning modes that emphasize the key roles of libraries in facilitating learning. In addition, some education experts have claimed that transmitting knowledge to learners is not the only…

  13. Implementation of a Detailed List of Subject Headings on Mormons and Mormonism within the Library of Congress Subject Heading System.

    Science.gov (United States)

    Birkinshaw, Scott B.; Chang, Stella

    Libraries in Utah have collected large numbers of materials on Mormonism and related topics and have a need to provide more detailed subject headings for these materials which the Library of Congress has lumped together under "Mormons and Mormonism." A list of subject headings which was developed for this purpose by a Committee of the…

  14. Generation of the library of neutron cross sections for the Record code of the Fuel Management System (FMS)

    International Nuclear Information System (INIS)

    Alonso V, G.; Hernandez L, H.

    1991-11-01

    On the basis of the library structure of the RECORD code a method to generate the neutron cross sections by means of the ENDF-B/IV database and the NJOY code has been developed. The obtained cross sections are compared with those of the current library which was processed using the ENDF-B/III version. (Author)

  15. Development of fine-group (315n/42γ) cross section library ENDL3.0/FG for fusion-fission hybrid systems

    International Nuclear Information System (INIS)

    Zeng Qin; Zou Jun; Xu Dezhen; Jiang Jieqiong; Wang Minghuang; Wu Yican; Qiu Yuefeng; Chen Zhong; Chen Yan

    2011-01-01

    To improve the accuracy of the neutron analyses for subcritical systems with thermal fission blanket, a coupled neutron and photon (315 n + 42γ) fine-group cross section library HENDL3.0/FG based on ENDF/B-Ⅶ. 0 has been produced by FDS team. In order to test the availability and reliability of the HENDL3.0/FG data library, shielding and critical safety benchmarks were performed with VisualBUS code. The testing results indicated that the discrepancy between calculation and experimental values of nuclear parameters fell in a reasonable range. (authors)

  16. IFLA General Conference, 1986. Pre-Conference Seminar on Automated Systems for Access to Multilingual and Multiscript Library materials: Problems and Solutions. Papers.

    Science.gov (United States)

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar which considered problems and solutions regarding automated systems for access to multilingual and multiscript library materials was held as a pre-session before the IFLA general conference in 1986. Papers presented include: (1) "Romanized and Transliterated Databases of Asian Language Materials--History, Problems, and…

  17. The E-rate Program and Libraries and Library Consortia, 2000-2004: Trends and Issues

    Directory of Open Access Journals (Sweden)

    Paul T. Jaeger

    2005-06-01

    Full Text Available The E-rate program has provided tremendous benefits to libraries, allowing many libraries and library systems to acquire technological equipment and services that would otherwise be too expensive, increasing the availability of public Internet access through libraries. This article analyzes the data related to the E-rate program and the discounts that it has provided to libraries and library systems between 2000 and 2004. By examining the E-rate data in a longitudinal manner, this article explores the trends in the application for and the provision of E-rate discounts to libraries and library consortia at national and state levels. The data suggest that, despite a number of controversies over the years, the program has provided a significant level of support for libraries and library consortia.

  18. Using a Recommender System and Hyperwave Attributes To Augment an Electronic Resource Library.

    Science.gov (United States)

    Fenn, B.; Lennon, J.

    There has been increasing interest over the past few years in systems that help users exchange recommendations about World Wide Web documents. Programs have ranged from those that rely totally on user pre-selection, to others that are based on artificial intelligence. This paper proposes a system that falls between these two extremes, providing…

  19. Identification of specific requirements for a NASA aerospace law information system and identification of the acquisition requirements for an aerospace law collection for the NASA law library

    Science.gov (United States)

    Morenoff, J.; Roth, D. L.; Singleton, J. W.

    1972-01-01

    The study to develop, implement, and maintain a space law library and information system is summarized. The survey plan; major interviews with individuals representative of potential sources, users and producers of information related to aerospace law; and system trade-off analyses are discussed along with the NASA/RECON system capability. The NASA publications of STAR and IAA are described, and the NASA legal micro-thesaurus is included.

  20. Library news

    CERN Multimedia

    CERN Library

    2010-01-01

    The CERN Library has been providing electronic access to the "Techniques de l'Ingénieur" database for the past 8 months. As a reminder, this is a multidisciplinary database of over 4000 technical and scientific articles in French, covering a broad range of topics such as mechanical engineering, safety, electronics and the environment. In a few simple steps, you can create your own account, select the types of documents you are interested in and configure your settings so as to receive alerts when articles in your field of activity are published. You can now access this resource from outside CERN using the "remote access to electronic resources" service. Further information is available here. Direct access to the database. Remote access to electronic resources. If you have any questions or comments, don't hesitate to contact us at: library.desk@cern.ch.

  1. Library Benchmarking

    Directory of Open Access Journals (Sweden)

    Wiji Suwarno

    2017-02-01

    Full Text Available The term benchmarking has been encountered in the implementation of total quality (TQM or in Indonesian termed holistic quality management because benchmarking is a tool to look for ideas or learn from the library. Benchmarking is a processof measuring and comparing for continuous business process of systematic and continuous measurement, the process of measuring and comparing for continuous business process of an organization to get information that can help these organization improve their performance efforts.

  2. Virtual Libraries: Service Realities.

    Science.gov (United States)

    Novak, Jan

    This paper discusses client service issues to be considered when transitioning to a virtual library situation. Themes related to the transitional nature of society in the knowledge era are presented, including: paradox and a contradictory nature; blurring of boundaries; networks, systems, and holistic thinking; process/not product, becoming/not…

  3. Evaluated nuclear data library

    International Nuclear Information System (INIS)

    Howerton, R.J.; Dye, R.E.; Perkins, S.T.

    1981-01-01

    The Lawrence Livermore National Laboratory (LLNL) collection of evaluated data for neutron-, photon-, and charged-particle-induced reactions is maintained in a computer-oriented system. In this report we recount the history of Evaluated Nuclear Data Library, describe the methods of evaluation, and give examples of input and output representation of the data

  4. Rx for Library Management.

    Science.gov (United States)

    Cage, Alvin C.

    1986-01-01

    Examines recent developments in field of systems theory that can improve library management practices by broadening perspective in which these problems may be approached. The concept of organizational life cycles is discussed and a paradigm for application of its principles is developed. An Rx decision chart is appended. (9 references) (EJS)

  5. Flexible digital library search

    NARCIS (Netherlands)

    Windhouwer, M.; Schmidt, A.; Zwol, van R.; Petkovic, M.; Blok, H.E.; Dahanayake, A.; Gerhardt, W.

    2003-01-01

    In this chapter the development of a specialised search engine for a digital library is described. The proposed system architecture consists of three levels: the conceptual, the logical and the physical level. The conceptual level schema enables by its exposure of a domain specific schema

  6. The Effects of Computer Anxiety and Technostress, as Functions of Resistance to Change, on the Staff of the 18 Founding OhioLINK Libraries as the OhioLINK Automated System Is Initiated.

    Science.gov (United States)

    Popovich, Donna

    This descriptive study surveys the staff of all 18 founding member libraries of OhioLINK to see whether or not they prefer the new system or the old one and why. The purpose of the study is to determine if resistance to change, computer anxiety and technostress can be found in libraries converting their automated systems over to the OhioLINK…

  7. Bacterial diversity analysis of Huanglongbing pathogen-infected citrus, using PhyloChip and 16S rRNA gene clone library sequencing

    Energy Technology Data Exchange (ETDEWEB)

    Shankar Sagaram, U.; DeAngelis, K.M.; Trivedi, P.; Andersen, G.L.; Lu, S.-E.; Wang, N.

    2009-03-01

    between the relative abundance, species richness and phylogenetic diversity of the microbial communities associated with the leaf midribs of HLB symptomatic and asymptomatic citrus trees were investigated using high-density 16S rDNA microarray PhyloChip and 16S rRNA gene clone library methods.

  8. Intelligent Libraries and Apomediators: Distinguishing between Library 3.0 and Library 2.0

    Science.gov (United States)

    Kwanya, Tom; Stilwell, Christine; Underwood, Peter G.

    2013-01-01

    Using the "point oh" naming system for developments in librarianship is attracting debate about its appropriateness, basis and syntax and the meaning and potential of Library 2.0. Now a new term, Library 3.0, has emerged. Is there is any significant difference between the two models? Using documentary analysis to explore the terms, the…

  9. Gene Profiling in Patients with Systemic Sclerosis Reveals the Presence of Oncogenic Gene Signatures

    Directory of Open Access Journals (Sweden)

    Marzia Dolcino

    2018-03-01

    Full Text Available Systemic sclerosis (SSc is a rare connective tissue disease characterized by three pathogenetic hallmarks: vasculopathy, dysregulation of the immune system, and fibrosis. A particular feature of SSc is the increased frequency of some types of malignancies, namely breast, lung, and hematological malignancies. Moreover, SSc may also be a paraneoplastic disease, again indicating a strong link between cancer and scleroderma. The reason of this association is still unknown; therefore, we aimed at investigating whether particular genetic or epigenetic factors may play a role in promoting cancer development in patients with SSc and whether some features are shared by the two conditions. We therefore performed a gene expression profiling of peripheral blood mononuclear cells (PBMCs derived from patients with limited and diffuse SSc, showing that the various classes of genes potentially linked to the pathogenesis of SSc (such as apoptosis, endothelial cell activation, extracellular matrix remodeling, immune response, and inflammation include genes that directly participate in the development of malignancies or that are involved in pathways known to be associated with carcinogenesis. The transcriptional analysis was then complemented by a complex network analysis of modulated genes which further confirmed the presence of signaling pathways associated with carcinogenesis. Since epigenetic mechanisms, such as microRNAs (miRNAs, are believed to play a central role in the pathogenesis of SSc, we also evaluated whether specific cancer-related miRNAs could be deregulated in the serum of SSc patients. We focused our attention on miRNAs already found upregulated in SSc such as miR-21-5p, miR-92a-3p, and on miR-155-5p, miR 126-3p and miR-16-5p known to be deregulated in malignancies associated to SSc, i.e., breast, lung, and hematological malignancies. miR-21-5p, miR-92a-3p, miR-155-5p, and miR-16-5p expression was significantly higher in SSc sera compared to

  10. BioSmalltalk: a pure object system and library for bioinformatics.

    Science.gov (United States)

    Morales, Hernán F; Giovambattista, Guillermo

    2013-09-15

    We have developed BioSmalltalk, a new environment system for pure object-oriented bioinformatics programming. Adaptive end-user programming systems tend to become more important for discovering biological knowledge, as is demonstrated by the emergence of open-source programming toolkits for bioinformatics in the past years. Our software is intended to bridge the gap between bioscientists and rapid software prototyping while preserving the possibility of scaling to whole-system biology applications. BioSmalltalk performs better in terms of execution time and memory usage than Biopython and BioPerl for some classical situations. BioSmalltalk is cross-platform and freely available (MIT license) through the Google Project Hosting at http://code.google.com/p/biosmalltalk hernan.morales@gmail.com Supplementary data are available at Bioinformatics online.

  11. FOND-2.2-evaluated nuclear data library for constants sets generation at ABBN constants providing system

    International Nuclear Information System (INIS)

    Koscheev, V.N.; Nikolaev, M.N.; Tsiboulia, A.M.

    2002-01-01

    The library FOND-2.2 of evaluated nuclear data files, which was created at the ABBN laboratory of IPPE, is described. FOND-2 library is the basic nuclear data source used for the preparation of group data sets with different energy structures. ABBN-93.1 group data set was retrieved from the FOND-2 data library and nowadays it is widely used in different applications, in neutronics calculations of different nuclear energetic installations with different kinds of neutron spectra, in radiation shielding calculation, and so on. (author)

  12. Development of Geographic Information Systems and their use in National Libraries of Russia

    Directory of Open Access Journals (Sweden)

    Natalya Kotelnikova

    2005-04-01

    Full Text Available By the end of the 20th century cartography has amassed a vast array of information on major aspects of existence, interaction and the functioning of nature and society owing to vigorous automation and computerisation. These are applied for the purpose of creating geographic information systems (GIS. The main function of geographic information systems is the storage and use of computer (electronic maps and atlases. Geographic information systems differ from each other in their territorial levels (global, national, regional, municipal and others and by their subjects (land, cadastre, ecological, sea and other maps. The geographic information system, ‘Black Sea’ is cited as an example. This is an information-based project compiled with a view to taking decisions on the protection of the resources of the basin of the Black Sea. The Black Sea GIS was developed for use by governments, scientists, the general public, NGOs and the media for the following purposes: · planning for marine environment activities and impacts on a regional scale; · public awareness through training, education, workshops, lectures and media; · scientific analysis, modelling, ecological impact assessment, science planning. The main GIS components are designed to perform the following functions: · data input; · data storage and database management; · data analysis and processing; · interaction with the user (graphical/map editing; and · data output and presentation (plotting.

  13. An Analysis and Allocation System for Library Collections Budgets: The Comprehensive Allocation Process (CAP)

    Science.gov (United States)

    Lyons, Lucy Eleonore; Blosser, John

    2012-01-01

    The "Comprehensive Allocation Process" (CAP) is a reproducible decision-making structure for the allocation of new collections funds, for the reallocation of funds within stagnant budgets, and for budget cuts in the face of reduced funding levels. This system was designed to overcome common shortcomings of current methods. Its philosophical…

  14. Going with the flow: Implementing a workflow system to streamline acquisitions in a special library

    CSIR Research Space (South Africa)

    Halland, Y

    2004-05-01

    Full Text Available and used CSIRIS staff within the business units as “guinea pigs”. Some refinement was done on the basis of the feedback received from them before a few end-users were asked to test the system as well. The testing period was limited as CS had other...

  15. Clinical and academic use of electronic and print books: the Health Sciences Library System e-book study at the University of Pittsburgh

    Science.gov (United States)

    Wessel, Charles B; Czechowski, Leslie J

    2011-01-01

    Objectives: The purpose of the Health Sciences Library System (HSLS) electronic book (e-book) study was to assess use, and factors affecting use, of e-books by all patron groups of an academic health sciences library serving both university and health system–affiliated patrons. Methods: A web-based survey was distributed to a random sample (n = 5,292) of holders of library remote access passwords. A total of 871 completed and 108 partially completed surveys were received, for an approximate response rate of 16.5%–18.5%, with all user groups represented. Descriptive and chi-square analysis was done using SPSS 17. Results: Library e-books were used by 55.4% of respondents. Use by role varied: 21.3% of faculty reported having assigned all or part of an e-book for class readings, while 86% of interns, residents, and fellows reported using an e-book to support clinical care. Respondents preferred print for textbooks and manuals and electronic format for research protocols, pharmaceutical, and reference books, but indicated high flexibility about format choice. They rated printing and saving e-book content as more important than annotation, highlighting, and bookmarking features. Conclusions: Respondents' willingness to use alternate formats, if convenient, suggests that libraries can selectively reduce title duplication between print and e-books and still support library user information needs, especially if publishers provide features that users want. Marketing and user education may increase use of e-book collections. PMID:21753914

  16. Development of a system using the library of the Genie spectroscopy software and exchange of samples

    International Nuclear Information System (INIS)

    Lapolli, Andre L.; Munita, Casimiro S.

    2011-01-01

    One the great difficulties in using the NAA method is in regards to the time that the operator spends exchanging the samples after each measurement. It becomes a big problem in routine analyses when various chemical elements are determined and then each sample must be measured at different decay times. The application of the automatic sample exchanger reduces the time analysis by several hours and reduces the tedious manual operation. Then, the effective use of NAA depends on the availability of a suitable automatic sample changer. There are some systems that are sold commercially, however many laboratories can not acquire them because they are costly. This paper presents altered programs the G2KNAA.REX, which created a screen making possible automatic or manual acquisitions by calling the old program NAAACQ.rex for the procurement manual and the new program NAAACQ2.rex for automatic requisitions. In conclusion, as can be seen in the program lines, the synchronization to automation, which unites the three systems (the computer, the Canberra Set, the sample exchanger) is done in a timely manner. The system was tested and is functioning in a satisfactory manner. (author)

  17. Genome-wide screen for salmonella genes required for long-term systemic infection of the mouse.

    Directory of Open Access Journals (Sweden)

    2006-02-01

    Full Text Available A microarray-based negative selection screen was performed to identify Salmonella enterica serovar Typhimurium (serovar Typhimurium genes that contribute to long-term systemic infection in 129X1/SvJ (Nramp1(r mice. A high-complexity transposon-mutagenized library was used to infect mice intraperitoneally, and the selective disappearance of mutants was monitored after 7, 14, 21, and 28 d postinfection. One hundred and eighteen genes were identified to contribute to serovar Typhimurium infection of the spleens of mice by 28 d postinfection. The negatively selected mutants represent many known aspects of Salmonella physiology and pathogenesis, although the majority of the identified genes are of putative or unknown function. Approximately 30% of the negatively selected genes correspond to horizontally acquired regions such as those within Salmonella pathogenicity islands (SPI 1-5, prophages (Gifsy-1 and -2 and remnant, and the pSLT virulence plasmid. In addition, mutations in genes responsible for outer membrane structure and remodeling, such as LPS- and PhoP-regulated and fimbrial genes, were also selected against. Competitive index experiments demonstrated that the secreted SPI2 effectors SseK2 and SseJ as well as the SPI4 locus are attenuated relative to wild-type bacteria during systemic infection. Interestingly, several SPI1-encoded type III secretion system effectors/translocases are required by serovar Typhimurium to establish and, unexpectedly, to persist systemically, challenging the present description of Salmonella pathogenesis. Moreover, we observed a progressive selection against serovar Typhimurium mutants based upon the duration of the infection, suggesting that different classes of genes may be required at distinct stages of infection. Overall, these data indicate that Salmonella long-term systemic infection in the mouse requires a diverse repertoire of virulence factors. This diversity of genes presumably reflects the fact that

  18. Gene doping detection: evaluation of approach for direct detection of gene transfer using erythropoietin as a model system.

    Science.gov (United States)

    Baoutina, A; Coldham, T; Bains, G S; Emslie, K R

    2010-08-01

    As clinical gene therapy has progressed toward realizing its potential, concern over misuse of the technology to enhance performance in athletes is growing. Although 'gene doping' is banned by the World Anti-Doping Agency, its detection remains a major challenge. In this study, we developed a methodology for direct detection of the transferred genetic material and evaluated its feasibility for gene doping detection in blood samples from athletes. Using erythropoietin (EPO) as a model gene and a simple in vitro system, we developed real-time PCR assays that target sequences within the transgene complementary DNA corresponding to exon/exon junctions. As these junctions are absent in the endogenous gene due to their interruption by introns, the approach allows detection of trace amounts of a transgene in a large background of the endogenous gene. Two developed assays and one commercial gene expression assay for EPO were validated. On the basis of ability of these assays to selectively amplify transgenic DNA and analysis of literature on testing of gene transfer in preclinical and clinical gene therapy, it is concluded that the developed approach would potentially be suitable to detect gene doping through gene transfer by analysis of small volumes of blood using regular out-of-competition testing.

  19. Selective Constraints on Coding Sequences of Nervous System Genes Are a Major Determinant of Duplicate Gene Retention in Vertebrates.

    Science.gov (United States)

    Roux, Julien; Liu, Jialin; Robinson-Rechavi, Marc

    2017-11-01

    The evolutionary history of vertebrates is marked by three ancient whole-genome duplications: two successive rounds in the ancestor of vertebrates, and a third one specific to teleost fishes. Biased loss of most duplicates enriched the genome for specific genes, such as slow evolving genes, but this selective retention process is not well understood. To understand what drives the long-term preservation of duplicate genes, we characterized duplicated genes in terms of their expression patterns. We used a new method of expression enrichment analysis, TopAnat, applied to in situ hybridization data from thousands of genes from zebrafish and mouse. We showed that the presence of expression in the nervous system is a good predictor of a higher rate of retention of duplicate genes after whole-genome duplication. Further analyses suggest that purifying selection against the toxic effects of misfolded or misinteracting proteins, which is particularly strong in nonrenewing neural tissues, likely constrains the evolution of coding sequences of nervous system genes, leading indirectly to the preservation of duplicate genes after whole-genome duplication. Whole-genome duplications thus greatly contributed to the expansion of the toolkit of genes available for the evolution of profound novelties of the nervous system at the base of the vertebrate radiation. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  20. Pluripotency gene network dynamics: System views from parametric analysis.

    Science.gov (United States)

    Akberdin, Ilya R; Omelyanchuk, Nadezda A; Fadeev, Stanislav I; Leskova, Natalya E; Oschepkova, Evgeniya A; Kazantsev, Fedor V; Matushkin, Yury G; Afonnikov, Dmitry A; Kolchanov, Nikolay A

    2018-01-01

    Multiple experimental data demonstrated that the core gene network orchestrating self-renewal and differentiation of mouse embryonic stem cells involves activity of Oct4, Sox2 and Nanog genes by means of a number of positive feedback loops among them. However, recent studies indicated that the architecture of the core gene network should also incorporate negative Nanog autoregulation and might not include positive feedbacks from Nanog to Oct4 and Sox2. Thorough parametric analysis of the mathematical model based on this revisited core regulatory circuit identified that there are substantial changes in model dynamics occurred depending on the strength of Oct4 and Sox2 activation and molecular complexity of Nanog autorepression. The analysis showed the existence of four dynamical domains with different numbers of stable and unstable steady states. We hypothesize that these domains can constitute the checkpoints in a developmental progression from naïve to primed pluripotency and vice versa. During this transition, parametric conditions exist, which generate an oscillatory behavior of the system explaining heterogeneity in expression of pluripotent and differentiation factors in serum ESC cultures. Eventually, simulations showed that addition of positive feedbacks from Nanog to Oct4 and Sox2 leads mainly to increase of the parametric space for the naïve ESC state, in which pluripotency factors are strongly expressed while differentiation ones are repressed.