WorldWideScience

Sample records for system eno oyo

  1. NC application to production site; Seisan genba eno NC oyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-01-10

    Fuji Electric Co., Ltd. built for and delivered to the Shinto plant of The Yokohama Rubber Co., Ltd., a manufacturing process management system with NC (network computer) used as an on-site client. This is a core system for manufacturing in the unified logistics plan of The Yokohama Rubber Co., Ltd., and constituted of five servers and about 200 NC client terminals. The on-site client terminals require (1) environmental resistance, (2) easiness of maintenance, and (3) reduction in maintenance control operations, for example. For these requirements, the system employed on-site client terminals by using the features of NC such as (1) it excels in the environmental resistance because of no magnetic disk device and (2) it reduces maintenance and maintenance control operations because of the unified management of software by the servers. Expected from now on are a number of NC applications to manufacturing sites having similar requirements. (translated by NEDO)

  2. Application of photovoltaic generating system to electric power in large ship; Taiyoko hatsuden system no ogata senpaku eno oyo ni kansuru kento

    Energy Technology Data Exchange (ETDEWEB)

    Katagi, T.; Ogawa, S.; Nishikawa, E.; Hashimoto, T. [Kobe University of Mercantile Marine, Kobe (Japan); Ishida, K.

    1996-10-27

    This paper describes the design of electric power system in a ship with photovoltaic power generating system, to examine applicability of the photovoltaic power generating system to the inboard power source. It also discusses effectiveness of the system for sea environment. At first, the actual route of a car carrier, meteorological data, and quantity of power consumption were picked up from the deck logbook and engine logbook. Then, the installation area of photovoltaic arrays, the quantity of photovoltaic power generation derived from the quantity of solar radiation, and the capacities of batteries and inverters were calculated, to design the electric power system in the ship with photovoltaic power generation system. Moreover, the NOx and SOx emissions were compared between the present power system and the usual power system using diesel power generator, to discuss the effectiveness of the present system for sea environment. Consequently, it was found that the emission of NOx was reduced by about 33% and the emission of SOx was reduced by about 28% compared to the usual power system. The effectiveness for sea environment was confirmed. 9 refs., 5 figs., 4 tabs.

  3. Inverse LMI control design method and its application to position keeping system; Gyakumondai LMI seigyokei sekkeiho no kaihatsu to ichi hoji system eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, I.; Hayashi, T. [Mitsubishi Heavy Industries, Ltd., Tokyo (Japan)

    1997-10-01

    Recently, with a progress of robust control such as H{infinity} control, the application of a control system design considering a robustness of controlled system fluctuation to real machinery became possible. In addition, LMI (Linear Matrix Inequality) control system design method is superior to conventional H{infinity} control, although including various difficult elements. The design algorithm permitting the robust control system design of non-linear problems by giving controlled systems was thus developed by introducing an inverse LMI control design method. This design algorithm determines the solution of models outputting the deviation between a target value and controlled variables by solving the simultaneous equations composed of a linear matrix inequality under an impulse response constraint condition and that under an H{infinity} norm constraint condition. This technique allows to give a pending step response as control condition. The technique was confirmed to be effective by its application to the position keeping control problem of marine floating structures. 6 refs., 11 figs., 2 tabs.

  4. Advanced mineral and lithological mapping using high spectral resolution TIR data from the active CO2 remote sensing system; CO2 laser wo mochiita kosupekutoru bunkaino netsusekigai remote sensing data no ganseki kobutsu shikibetsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Okada, K. [Sumitomo Metal Mining Co. Ltd., Osaka (Japan); Hato, M. [Earth Remote Sensing Data Analysis Center, Tokyo (Japan); Cudahy, T.; Tapley, I.

    1997-05-27

    A study was conducted on rock/mineral mapping technology for the metal ore deposit survey using MIRACO2LAS, an active type thermal infrared ray remote sensing system which was developed by CSIRO of Australia and is now the highest in spectral resolution in the world, and TIMS of NASA which is a passive type system. The area for the survey is the area of Olary/Broken Hill and Mt. Fitton of Australia. A good correlation is seen between the ground reflectance measured by MIRACO2LAS and the value measured by the chamber CO2 laser of rocks sampled at the above-mentioned area. In case that the width of spectral characteristics is below 300nm, the inspection ability by MIRACO2LAS`s high spectral resolution is more determined in mineral mapping as compared with TIMS which is large in band width. Minerals mapped using MIRACO2LAS are quartz, talc, amphibole, hornblende, garnet, supessartine, dolomite, magnesite, etc. 4 refs., 3 figs.

  5. New oxide magnetic material for sub-4K cryocoolers; Sankabutsu jiseitai no chikureizai eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Numazawa, T.; Sato, A.; Wada, H. [National Research Inst. of Metals, Tokyo (Japan). Tsukuba Magnet Lab.; Arai, O. [Tokyo Inst. of Tech., Tokyo (Japan)

    2000-05-29

    Aiming at improvement of refrigerating performance below 4 K, magnetic cryocoolers with large heat capacity in this region were examined. Noticing comparatively weak and simple magnetic interaction of oxide magnetic materials, possible substances which have large volume specific heat below 4 K were searched, and found perovskite system rare earth magnetic material GdAlO{sub 3}. Though GdAlO{sub 3} polycrystal shows {lambda} type ferromagnetic transition directly below 4 K, magnetic entropy of Gd is sufficiently maintained, and Gd has a large specific heat peak in 3 {approx} 4 K. Comparing with practical magnetic cryocooler HoCu{sub 2}, this GdAlO{sub 3} was found to have over 3.5 times specific heat. (NEDO)

  6. Reactive transport model of multiple air pollutants in non-conservation system and its application to prediction of the concentrations of benzene and toluene in the atmosphere; Taseibun taiki osen busshitsu no hihozongata yuso hanno model to sono benzene oyobi toluene nodo yosoku eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Iinuma, K. [Tohoku University, Sendai (Japan)

    1998-07-10

    A general reactive transport model in non-conservation systems is developed, in order to elucidate the phenomena involved in the transport and reactions of multiple pollutants, and applied to prediction of concentrations of benzene and toluene in the atmosphere. The single pollutant model is used to analyze the processes of benzene formation, transport, diffusion and removal, which gives an estimated maximum equilibrium concentration of around 23{mu}g/m{sup -3}. Next, the two-pollutant model, which considers the transport and forward/reverse reaction processes for 2 types of pollutants, is developed to analyze a toluene/cresol system. The results indicate that the steady-state concentration distributions change, competing with the forward reaction. Finally, the 4-pollutant model, which can analyze the transport and 12 reaction processes, is developed to analyze the conversion process of toluene into benzaldehyde, with 2 intermediate radical species and 6 reaction rate constants. There is a dynamic equilibrium in the spatial concentration distributions in a 30 to 80km area, 10h after evolution of toluene. 22 refs., 7 figs., 2 tabs.

  7. Circulating Blood eNOS Contributes to the Regulation of Systemic Blood Pressure and Nitrite Homeostasis

    Science.gov (United States)

    Wood, Katherine C.; Cortese-Krott, Miriam M.; Kovacic, Jason C.; Noguchi, Audrey; Liu, Virginia B.; Wang, Xunde; Raghavachari, Nalini; Boehm, Manfred; Kato, Gregory J.; Kelm, Malte; Gladwin, Mark T.

    2013-01-01

    Objective Mice genetically deficient in endothelial nitric oxide synthase (eNOS−/−) are hypertensive with lower circulating nitrite levels, indicating the importance of constitutively produced nitric oxide (NO•) to blood pressure regulation and vascular homeostasis. While the current paradigm holds that this bioactivity derives specifically from expression of eNOS in endothelium, circulating blood cells also express eNOS protein. A functional red cell eNOS that modulates vascular NO• signaling has been proposed. Approach and Results To test the hypothesis that blood cells contribute to mammalian blood pressure regulation via eNOS-dependent NO• generation, we cross-transplanted WT and eNOS−/− mice, producing chimeras competent or deficient for eNOS expression in circulating blood cells. Surprisingly, we observed a significant contribution of both endothelial and circulating blood cell eNOS to blood pressure and systemic nitrite levels, the latter being a major component of the circulating NO• reservoir. These effects were abolished by the NOS inhibitor L-NAME and repristinated by the NOS substrate L-Arginine, and were independent of platelet or leukocyte depletion. Mouse erythrocytes were also found to carry an eNOS protein and convert 14C-Arginine into 14C-Citrulline in a NOS-dependent fashion. Conclusions These are the first studies to definitively establish a role for a blood borne eNOS, using cross transplant chimera models, that contributes to the regulation of blood pressure and nitrite homeostasis. This work provides evidence suggesting that erythrocyte eNOS may mediate this effect. PMID:23702660

  8. Application to the field of medical and welfare with fluid power; Fluid power servo no iryo fukushi bun`ya eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayakawa, Y. [Nara Technical Coll., Nara (Japan)

    1999-05-15

    Reported herein are medical and welfare apparatuses activated by fluid power servos. Reference is also made to equipment now undergoing research and development. Numerous fluid power-driven welfare and caring apparatuses have been developed, some of which are named below. In an externally powered prosthetic leg, a rocking type hydraulic servo actuator is attached on the knee section. In this device, switching is performed between an active operation in which much torque is required for instance going up and down a stairway and a passive operation in which less torque is required for instance walking on a flat place, this for increased energy efficiency. In an externally powered orthosisis, an exoskeleton is installed on the lower extremities of a patient suffering from the paralysis of both legs, and enables physical exercises for rehabilitation. Such devices are controlled by one of the two methods, the master-slave method or the manual method. Devices for the transfer of patients include an in-home bathing assist system, powered assist suit for caring, powered assist chair, movable lift for transfer, pneumatic Rubbertuator-driven rehabilitation equipment, walk training device, and wavy motion pneumatic vibrator. (NEDO)

  9. Surface observation by scanning laser microscope. 1. Application to materials testing; Laser kenbikyo ni yoru hyomen kansatsu. 1. Sozai shiken eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, A. [Toyota Technological Institute, Aichi (Japan)

    1994-03-20

    This paper introduces examples of applications of a laser microscope to material testing, and describes superiority of the laser microscope to the conventional measuring means. A scanning electron microscope has such problems as the only availability for observation in vacuum, necessity of gold plating onto non-electrically conductive materials, and electron beams damaging non-heat resistant materials. The laser microscope eliminates all of these problems, and is capable of observation with higher magnification and focal depths. The in-situ observation system using the prototype laser microscope has such a construction that a material tester is installed beneath an object lens of the microscope, and the test piece surface acted with tensile stress is observed by inserting the object lens into an observation window. In a TiAl bending test, changes on the test piece surface were observed of generation of a large number of micro cracks and fractures as a result of combination of these cracks. A three-dimensional bird`s eye view chart reproducing fine irregularities of smaller than 1 {mu}m was obtained in observation of the fatigue fractured wave faces of alumina ceramics by using the focus movement memorizing function of the laser microscope. 9 refs., 9 figs.

  10. Application of distributed control system to power generator controlling system; Hatsudenki seigyokei eno bunsanseigyo hoshiki eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Morioka, Y. [Kansai Electric Power Co. Inc., Osaka (Japan)

    1999-05-10

    The method to apply a distributed control system to power generator controlling systems has been studied, where the distributed control is based on the H infinitive control for a multi-machine system as the model for the whole system to be controlled. The digital simulation was also conducted to verify the system. In the distributed control system, one generator is taken as one sub-system and the portions representing its interactions with other generators are taken as disturbances to the generator in question, because the H infinitive control is used. It is designed to stabilize the system from the disturbances by applying the disturbance-preventing control of the H infinitive control. The digital simulation was conducted for a 3-machine loop system and system with 5 machines connected in series. It is confirmed that the proposed method shows better controllability than the conventional one, securing robustness to changed system conditions and synergistic effects when applied to plural machines. (NEDO)

  11. Effect of rural transportation system on agricultural productivity in Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Ezekiel Olukayode Idowu

    2012-04-01

    Full Text Available Food farming in Oyo North, Nigeria is characterised by an increasing use of Intermediary Mode of Transportation (IMT to ease inputs and outputs mobility and farm access. To assess the influence on food farmer’s productivity, a random sample of 230 respondents was selected and data collected on their socio-economic and farm specific characteristics. Descriptive statistics, Herfindhal Index and Technical Efficiency Approach were used to analyse the data. The results indicate that majority of food farmers were in their middle age with mean age of 50 years and most of them used one plot at a location between 5 and 10km to their village of residence. They acquired land by inheritance and practiced intensive crop diversification as risk management strategy. The transportation modes used in addition to walking include bicycle, motorcycle, and car with increasing trend in the use of motorcycle. The mean Technical Efficiency (TE of food farmers was 0.82 with significant inefficiency effects. The inefficiency analysis indicates positive effect of distance, crop diversification and un-tarred type of road on farmer’s productivity, while poor level of education among farmers, use of bicycle; trekking and weekly working time negatively affect farmer’s efficiency. The negative effect of trekking and use of bicycle and the excess working time suggest the adoption of more IMT of motorized type to optimize farming time and increase farmer’s productivity.

  12. Coping with leveling up information system of electric power; `Denryoku no kodo jofoka system` eno torikumi

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    This article is an outline of the highly informational systematization project of Tohoku Electric Power Company. The company planned and determined the concept of a high informational system in 1988 and is carrying forward its realization by dividing the concept into the first period of preparation of the base, the second period of establishment of systems integration and the third period of progress and leveling up. Regarding the third period of the high informational system project which started from FY 1995, it is decided to reexamine drastically the way which has been taken for carrying forward information systematization and to intend further improvement of management efficiency. In this latest project of transforming the company highly informational, the following 3 points are adopted as the basic policies, namely increase of efficiency by drastic reexamination of the transverse service treatment process, improvement of productivity of white collars, and positive utilization of such information techniques as the down-sizing technology, etc.. In its concrete execution, these points affect widely and after reexamination of service treatment which includes organizational and operational systems such as organizations, authorities, etc. centering around in house transverse service processes, the systematization will be planned. It will also be considered to make information accessible jointly for the personnel concerned by electric mail, etc. and to utilize positively the trunk data which large computers have. 1 fig., 2 tabs.

  13. Ifa Divination Trays from Isale-Oyo

    Directory of Open Access Journals (Sweden)

    O. I. Pogoson

    2011-10-01

    Full Text Available This paper is a study of the images and patterns on ifa divination trays from Isale-Oyo. The paper also explains some ifa paraphernalia. The paper establishes that ifa trays from Isale-Oyo bear distinctive features when compared with other ifa trays in Yorubaland such as those of Ijebu and Osogbo. Central to this study is the iconographic description and interpretations of Isale-Oyo divination trays. Data were collected through direct interviews with divination tray owners. Photographs of the trays were also taken. The investigation revealed that:Divination trays from Isale-Oyo combine features found on both Ijebu and Osogbo trays.The Esu figure continues to occupy the top central position on Oyo divination trays, even though with representational variations elsewhere in Yorubaland.Some divination trays in Oyo carry no decorations on their borders, and in some recent cases, ceramic plates are now used for divination.Este artigo trata dos padrões e imagens dos tabuleiros de adivinhação ifa usados em Isale-Oyo (Nigéria, bem como de outros objectos religiosos associados. Argumenta-se que os tabuleiros ifa de Isale-Oyo têm características que os distinguem de outros tabuleiros ifa da região yoruba, como os de Ijebu e Osogbo. A descrição e a interpretação da iconografia dos tabuleiros de adivinhação constituem o foco deste estudo. Os dados discutidos foram recolhidos através de entrevistas a proprietários de tabuleiros de adivinhação e fotografias de tabuleiros. Conclui-se que:Os tabuleiros de adivinhação de Isale-Oyo combinam traços dos tabuleiros de Ijebu e dos tabuleiros de Osogbo.A figura de Esu continua a ocupar a posição central superior nos tabuleiros de adivinhação de Oyo, embora se registem variações na sua representação noutras zonas do território yoruba.Alguns tabuleiros de adivinhação de Oyo não possuem qualquer decoração nos frisos, e nalguns casos recentes vêm sendo utilizados na adivinhação pratos

  14. Winning strategies: A case study of Oyo State Lottery, Nigeria ...

    African Journals Online (AJOL)

    In this study, we investigated three common lottery strategies: random, low and high frequency strategies, usually employed by lottery players. The Oyo State Lottery, a type of lottery in Oyo State, Nigeria was used as a case study. For the three strategies, we considered whether the selection of numbers in Oyo State lottery ...

  15. Electronic and photonic application of C60. Denki denshi kogaku eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, T. (NTT Interdisciplinary Research Laboratories, Tokyo (Japan))

    1994-01-20

    This paper describes the following matters related to application of photonic and electronic characteristics of Fullerene: C60 crystals precipitated from benzene aqueous solution have an fcc structure and an hcp structure mixed, with its mixing ratio depending on hysteresis of test specimens. Mono-crystal thin films of C60 and C70 can be formed on mica substrates by means of a vacuum deposition process. On a CaF2 (111) substrate under normal temperature, a C60 thin film with the fcc structure grows in a single direction in the plane having its (111) plane paralleled with the substrate face. However, on a substrate under elevated temperatures, the film grows in two different directions in the plane, making grid conformity better in C60 and CaF2. Thin films of C60 and C70 transfer to a low temperature phase at a temperature of about 260 K or lower. Carbon nanocapsules containing LaC2 monocrystals can be obtained from arc discharge of a graphite electrode containing La oxides. The paper introduces nonlinear optical characteristics of C60 and C70 thin films, and light conductive polymers doped with C6O and C70. 39 refs., 10 figs.

  16. [Remodeling of the cardiovascular system and development of chronic kidney disease in patients with metabolic syndrome and obesity: role of eNOS, subunit p22-phox of NADPH-oxidase and MTHFR genes].

    Science.gov (United States)

    Saginova, E A; Galliamov, M G; Balatskiĭ, A V; Kolotvin, A V; Severova, M V; Samokhodskaia, L M; Fomin, V V; Krasnova, T N; Mukhin, N A

    2012-01-01

    To examine contribution of polymorphisms of genes of endothelial NO-synthase (eNOS), NADPH-oxidase and methylenetetrahydrofolate reductase (mTHFR) to development of remodeling of cardiovascular system and chronic disease of the kidneys (CDK) in patients with metabolic syndrome (mS) and obesity. MATERIAL AND METHODS. Standard clinical and device examinations were made and polymorphisms C242T of gene of subunit p22-phox of NADPH-oxidase, G894T of gene of eNOS and C677T of gene of MTHFR were studied in 66 MS patients (49 males and 17 females, age 19-62 years. The presence of even one prognostically poor allele variants of the genes studied was registered in 83 examinees. The genotype 242TTp22-phox of NADPH-oxidase subunit was associated with the highest insulin resistance, allele 894T of gene eNOS- with reduced glomerular filtration rate and progression of left ventricular hypertrophy. Polymorphism of the genes the products of which modulate endothelial function can be considered as potential predictors of severity of MS target organs impairment.

  17. Stress coping strategies among agricultural extension agents in Oyo ...

    African Journals Online (AJOL)

    The study examined the coping strategies used by extension agents A total of seventy 72agricultural extension agents were randomly sampled out of 288 agricultural extension agents from the four zones (Saki, Oyo, Ogbomosho, and Ibadan) in the Oyo State Agricultural Development Programme (OYSADEP).

  18. Role of women in wildlife development in Oyo State, Nigeria ...

    African Journals Online (AJOL)

    This study was carried out in some selected communities nearer to natural resources and protected areas in Oyo State. These are Sepeteri and Igboho that shared boundaries with Old Oyo National Park; Asejire and Oranyan markets nearer to Olokemeji forest via Ibadan. The activities considered are general information on ...

  19. Endothelin-1 Overexpression Improves Renal Function in eNOS Knockout Mice

    Directory of Open Access Journals (Sweden)

    Oleg Tsuprykov

    2015-10-01

    Full Text Available Background/Aims: To investigate the renal phenotype under conditions of an activated renal ET-1 system in the status of nitric oxide deficiency, we compared kidney function and morphology in wild-type, ET-1 transgenic (ET+/+, endothelial nitric oxide synthase knockout (eNOS-/- and ET+/+eNOS-/- mice. Methods: We assessed blood pressure, parameters of renal morphology, plasma cystatin C, urinary protein excretion, expression of genes associated with glomerular filtration barrier and tissue remodeling, and plasma metabolites using metabolomics. Results: eNOS-/- and ET+/+eNOS-/- mice developed hypertension. Osteopontin, albumin and protein excretion were increased in eNOS-/- and restored in ET+/+eNOS-/- animals. All genetically modified mice developed renal interstitial fibrosis and glomerulosclerosis. Genes involved in tissue remodeling (serpine1, TIMP1, Col1a1, CCL2 were up-regulated in eNOS-/-, but not in ET+/+eNOS-/- mice. Plasma levels of free carnitine and acylcarnitines, amino acids, diacyl phosphatidylcholines, lysophosphatidylcholines and hexoses were descreased in eNOS-/- and were in the normal range in ET+/+eNOS-/- mice. Conclusion: eNOS-/- mice developed renal dysfunction, which was partially rescued by ET-1 overexpression in eNOS-/- mice. The metabolomics results suggest that ET-1 overexpression on top of eNOS knockout is associated with a functional recovery of mitochondria (rescue effect in β-oxidation of fatty acids and an increase in antioxidative properties (normalization of monounsaturated fatty acids levels.

  20. Differential Perception of Counselling Psychologists' Duties to Oyo ...

    African Journals Online (AJOL)

    Differential Perception of Counselling Psychologists' Duties to Oyo State Broadcasting Corporation. ... that public enlighment programmes should be sponsored by counselling psychologists to create adequate awareness of their relevance to other work settings apart from educational and clinical traditional work settings.

  1. Winning Strategies: A Case Study of Oyo State Lottery, Nigeria

    African Journals Online (AJOL)

    PROF. OLIVER OSUAGWA

    2014-06-01

    ui.edu.ng, seramide2003@yahoo.co.uk. Abstract. In this study, we investigated three common lottery strategies: random, low and high frequency strategies, usually employed by lottery players. The Oyo State Lottery, a type of.

  2. Suitability assessment and mapping of Oyo State, Nigeria, for rice cultivation using GIS

    Science.gov (United States)

    Ayoade, Modupe Alake

    2017-08-01

    Rice is one of the most preferred food crops in Nigeria. However, local rice production has declined with the oil boom of the 1970s causing demand to outstrip supply. Rice production can be increased through the integration of Geographic Information Systems (GIS) and crop-land suitability analysis and mapping. Based on the key predictor variables that determine rice yield mentioned in relevant literature, data on rainfall, temperature, relative humidity, slope, and soil of Oyo state were obtained. To develop rice suitability maps for the state, two MCE-GIS techniques, namely the Overlay approach and weighted linear combination (WLC), using fuzzy AHP were used and compared. A Boolean land use map derived from a landsat imagery was used in masking out areas currently unavailable for rice production. Both suitability maps were classified into four categories of very suitable, suitable, moderate, and fairly moderate. Although the maps differ slightly, the overlay and WLC (AHP) approach found most parts of Oyo state (51.79 and 82.9 % respectively) to be moderately suitable for rice production. However, in areas like Eruwa, Oyo, and Shaki, rainfall amount received needs to be supplemented by irrigation for increased rice yield.

  3. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field / Study on a new production process of functional thin films suitable for recycling and its application to colored glasses (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / recycle ni tekishita kinosei usumaku no shinki seizoho to chakushoku glass eno oyo ni kansuru kenkyu kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper described the fiscal 1997 result. Using organic pigment and dye, the basic composition of sol-gel colored coating liquid for glass bottles and sheets was selected to clarify characteristics of gel films. Moreover, chemical modification and optical sensitivity of metallic alkoxide were studied to obtain a trially produced colored glass bottle use coating liquid. As to sheet glass, strength of the gel film is low, which requires further improvement. In relation to optical sensitive gel films, the reaction of various metallic alkoxides and {beta}-diketones was discussed to clarify chemical reactivity with utltaviolet rays. Trial design was also conducted of the coating equipment. The paper examined by literature the present status of recycling systems of colored glass bottles and the technical development in European countries. The problem is a method to wash colored bottles for sterilization. To make colored films durable, it is necessary to form films by organic-inorganic hybrid thin films. Also discussed was a possibility of changing the washing method by administrative guidance. 27 refs., 62 figs., 42 tabs.

  4. Incidence of Poverty among Fish Farming Households in Oyo and ...

    African Journals Online (AJOL)

    The objective of the study is to analyse poverty among fish farming households based on functioning and capabilities in the study area. The study covered both Oyo and Osun states of Nigeria. Primary data were collected via structured questionnaire. The study employed multistage sampling technique. In all, a total of 280 ...

  5. Assessment of management practices for ungulates in old Oyo ...

    African Journals Online (AJOL)

    Assessment of the habitat management practices was investigated for four months to identify the various management strategies and threats affecting ungulates in Old Oyo National Park. The methods used for the study are observation techniques and questionnaire administration and data were analyzed descriptively.

  6. USE OF ICT IN SELECTED LIBRARIES IN IBADAN, OYO STATE ...

    African Journals Online (AJOL)

    USE OF ICT IN SELECTED LIBRARIES IN IBADAN, OYO STATE, NIGERIAN. O.E.L Eguavoen and B.M Adeyemi. Page 2 education and human capacity building, there are significant patterns of change because ICTs have made it necessary for significant change in traditional concepts of teaching and learning, as well as ...

  7. Taenia saginata cysticercosis in Oyo State, Nigeria | Faleke ...

    African Journals Online (AJOL)

    A retrospective analysis of abattoir records in Oyo State of Nigeria showed that 0.45% the total 661,048 cattle carcasses inspected between 1989 and 1998 had Cysticercus bovis. Participatory meat inspection at Moniya slaughter slab in Ibadan between August and October, 2000 showed that 1.76% of the 1,413 carcasses ...

  8. Information needs of grasscutter farmers in Oyo State, Nigeria ...

    African Journals Online (AJOL)

    The study examined information needs of grasscutter farmers in Oyo State, Nigeria. Multistage sampling was used to select a total of 76 grasscutter farmers in the study area, as descriptive statistics such as frequency counts and percentages, as well as inferential statistics as Pearson product Moment Correlation (PPMC) ...

  9. Incidence speech defects in selected secondary schools of Oyo ...

    African Journals Online (AJOL)

    ... of various speech defects in five selected schools in Oyo states. The findings revealed that Speech disorders are common in the schools selected, but in various degrees. Articulatory defects are more common in the schools, followed by delayed speech, voice disorder and hearing loss, stuttering has the least occurrence.

  10. Geotechnical application of geological information on Kanto loam; Kanto loam ni okeru chishitsugaku no jiban kogaku eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    So, E. [Kanto Gakuin University, Yokohama (Japan)

    1998-02-01

    Quantitative relationship between the allophane clay minerals in the Kanto Loam is cited to describe the relationship between their stratigraphic features and geotechnical features. The Kanto Loam is a soil of weathered Diluvial volcanic ash deposited on hills and plateaus. The Tachikawa loam is the newest, followed by the Musashino loam, Shimosueyoshi loam, and Tama loam, named in the order from new to old. The Tachikawa and Musashino loams are relatively new loams formed approximately several tens of thousand years ago, and are different from each other in the percentage of allophanes (Ap), which is 50% or higher for the Tachikawa loam and 40% or lower for the Musashino loam, and in the equivalent liquid limit (Ap equivalent index number), which is 115% or higher for the Tachikawa loam and 105% or lower for the Musashino loam. The new loam behaves like the Ap-governed volcanic ash while the old loam behaves like the alluvial clay. There is a linear, proportional relationship between Ap of the new loam and the geotechnical index number. The physical and dynamic behavior of the new loam is all under control of the aggregate structure formed of allophanic clay minerals, the loam behaving like sand when not disturbed and like clay when disturbed. The physical and dynamic properties are also discussed. 6 refs., 5 figs., 1 tab.

  11. Introduction of electric double layer capacitors in the solar-EV; Solar denki jidosha eno denki nijuso condenser no oyo

    Energy Technology Data Exchange (ETDEWEB)

    Fujinaka, M. [Tokyo Denki University, Tokyo (Japan)

    1996-10-27

    A basic experiment was carried out on a supplementary power supply, in which solar cells and electric double layer capacitors(EDLC) were combined for a DC/DC converter, for the use of a solar-electric vehicle (S-EV); and in actuality, an S-EV was loaded with the power supply with a running test conducted on a public road. The EDLC was found effective and useful for avoiding temporary lowering of voltage and momentary break. An electric supply was thereby made possible for an emergency light without the use of the DC/DC converter. However, in a tunnel or a night driving and in case of failure of the DC/DC converter, an operating time of only 7 minutes or so was affordable with the EDLC having a capacity of 100F. Moreover, particularly with a heavy load, it was impossible to maintain a voltage for many hours. Under the circumstances, an S-EV design would primarily require two sets of independent DC/DC converter loaded in the future. The EDLC, young after it was developed, still has a small energy density compared with a lead storage battery. Yet, an EDLC with a higher performance being developed, there is a possibility that it will be applied to S-EV`s by utilizing its characteristics such as a high efficiency and a long service life. 4 refs., 8 figs.

  12. Ultra-low light level imaging and its application to biosciences. Goku bijakuko imaging to sono seitai keisoku eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayakawa, T. (Hamamatsu Photonics K.K., Shizuoka (Japan))

    1992-12-05

    This paper describes an ultra-high sensitivity TV camera (photon counting camera) that can detect photons one by one and image them under ultra-low level light, and its application. Photographing under ultra-low level light has become possible by using a two-dimensional photon counting method. In observing movements of sea urchin roes before and after fertilization, fluidity of the membranes was quantified two-dimensionally and chronologically. In the example of the number of fluorescent molecules sealed in red blood cell ghosts, the number was imaged up to 10 molecules per ghost. Its most characteristic application would be to utilize its capability of imaging performance under as low light as incident photons would not almost superpose with each other in terms of space and time. The number of biological photons after about one week of sowing soybean seeds was several hundred photons per cm[sup 2]/S in a portion receiving abundance of light on a test sample. Other applications may include imaging Ca ions discharged during fertilization of the roes, chronological observation on process of active oxygen release from white blood cells in defective nutrition, and visualization of gene emergence utilizing luciferin-luciferase light emission. 8 refs., 9 figs.

  13. Altered Endometrial Expression of Endothelial Nitric oxide Synthase (eNOS) in women with Unexplained Recurrent Miscarriage and Infertility

    Science.gov (United States)

    Najafi, Tohid; Novin, Marefat Ghaffari; Ghazi, Reza; Khorram, Omid

    2012-01-01

    Background Endothelial nitric oxide synthase (eNOS) has diverse roles in the female reproductive system including a role in blastocyst implantation. Aberrant expression of eNOS could therefore be significant in the pathogenesis of disorders of implantation Materials and Methods eNOS protein and mRNA levels in the endometrium of women with recurrent miscarriages, unexplained infertility, and a control group was determined by compartmental quantitative immunohistochemistry and real time RT-PCR Results eNOS was immunolocalized to all layers of the endometrium and the vascular endothelium. eNOS protein expression was higher in glandular epithelium (P=0.004) and luminal epithelium (P=0.002) but not vascular endothelium and stroma (P=0.14) in women with recurrent miscarriage. Similarly, in women with unexplained infertility eNOS expression was significantly higher (Pinfertility compared with controls Conclusion Increased expression of eNOS in glandular and luminal epithelium of the endometrium in women with recurrent miscarriages and unexplained infertility suggests a detrimental effect of excess nitric oxide in endometrial receptivity and implantation PMID:22877939

  14. Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

    Science.gov (United States)

    Cerqueira, Fernanda M; Laurindo, Francisco R M; Kowaltowski, Alicia J

    2011-03-31

    Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR). Since treatment of mice with dinitrophenol (DNP) promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.

  15. Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

    Directory of Open Access Journals (Sweden)

    Fernanda M Cerqueira

    Full Text Available Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR. Since treatment of mice with dinitrophenol (DNP promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.

  16. Elasticities of resource- use in fish production: a case study of Oyo ...

    African Journals Online (AJOL)

    This paper examined the elasticities of resource use in fish production in Oyo agricultural zone, Oyo state. The data used is from a primary source. The instruments of data collection were structured questionnaire and in-depth interview. Multi-stage sampling technique was employed to sample 120 fish farmers. However ...

  17. Assessment of Users Information Needs and Satisfaction in Selected Seminary Libraries in Oyo State, Nigeria

    Science.gov (United States)

    Adekunjo, Olalekan Abraham; Adepoju, Samuel Olusegun; Adeola, Anuoluwapo Odebunmi

    2015-01-01

    The study assessed users' information needs and satisfaction in selected seminary libraries in Oyo State, Nigeria. This paper employed the descriptive survey research design, whereby the expost-facto was employed with a sample size of three hundred (300) participants, selected from six seminaries located in Ibadan, Oyo and Ogbomoso, all in Oyo…

  18. Fundamental study on PV system for floating water power device `Mighty Whale`; Okiai futaishiki haryoku sochi `Mighty Whale` eno taiyoko hatsuden sochi tosai no kihon kento

    Energy Technology Data Exchange (ETDEWEB)

    Washio, Y. [Japan Marine Science and Technology Center, Kanagawa (Japan)

    1996-10-27

    Studied in this paper is the use of a photovoltaic power generation system in combination with a wave energy conversion system to cover the latter`s weak point that it is available but intermittently. An offshore floating wave power device Mighty Whale (MW) is described, which will have a wave energy absorbing air chamber in the front and an anti-rocking float that looks like a whale in the rear. The movement, up and down and back and forth, of the water surface in the air chamber due to incident waves turns the power generator turbine. Out of the space available on the MW, a 200m{sup 2} area has been specified as the place suitable for the installation of solar cells capable of a maximum output of 20kW, for which specification the elevation of the sun has been taken into account. Batteries will routinely be supplied with power in parallel from the wave activated and photovoltaic devices, the supply will be limited when the batteries are fully charged, and an auxiliary power generator will be run in case of shortage. Power that can be provided by a 20kW solar array was calculated using actual data of sunshine on the slope for each array orientation, and required battery capacity was calculated for each solar array orientation. Important items for consideration for the insurance of reliability on the ocean include measures against salt damage, detailed designing of wiring, and coordinated operation of photovoltaic and wave activated power generation systems. 3 refs., 6 figs., 5 tabs.

  19. Crime-Reporting Practices Among Market Women in Oyo, Nigeria

    Directory of Open Access Journals (Sweden)

    Johnson Oluwole Ayodele

    2015-04-01

    Full Text Available Crime surveys of businesses have revealed that while crimes in which men were victims tend to be reported, those in which women were victims are likely to go unreported to the police. Understanding the reasons behind male reporting and female non-reporting is useful not only for collection of crime statistics but also for improving crime control competences of law enforcement agencies. This article examines the impact of crime involving market women on their crime-reporting practices in Oyo town, Oyo State, Nigeria. The study adopted quantitative and qualitative approaches. Copies of a questionnaire were administered to collect quantitative data from randomly selected 210 market women at Akesan, Sabo, and Mosadoba markets in Oyo town. Five focus group discussions, in-depth and key informant interviews were conducted to complement quantitative data. Both data were analyzed. The study found that cultural considerations stand between crime events that hurt the economic interests of women and their readiness to report to the police. Due to the very low confidence that market women have in the ability and willingness of the police to apprehend criminals, they prefer to internalize their losses, take their cases to traditional rulers who use “oro cult” to protect them against criminals, or approach available faith-based options such as churches and mosques. The article concludes that women have economy-enriching roles to play in the context of sustainable security. It therefore suggests that the government should address public safety to enable market women make their modest contribution to Nigeria’s economic development.

  20. Localization of the two constitutively expressed nitric oxide synthase isoforms (nNOS and eNOS) in the same cell types in the saccule maculae of the frog Rana pipiens by immunoelectron microscopy: evidence for a back-up system?

    Science.gov (United States)

    Heinrich, Ulf-Rüdiger; Lioudyno, Maria; Maurer, Jan; Mann, Wolf; Guth, Paul S; Förstermann, Ulrich

    2003-01-01

    There is growing evidence for a nitric oxide/cyclic GMP pathway of signal transduction in the vestibular system. Recently, two isoforms of nitric oxide (NO) synthase (nNOS and eNOS) and NO itself have been identified at the light microscopic level in the vestibulocochlear system of mice using specific antibodies and a new fluorescence indicator. In order to acquire more information about signal transduction and tissue modulation in this neuroepithelium at the cellular and subcellular levels, ultrathin sections of London Resin White-embedded saccule maculae of the frog Rana pipiens were incubated with various concentrations of commercially available antibodies to nNOS and eNOS. The immunoreactivity was visualized by a gold-labelled secondary antibody and the amount of the immunoreactions per microm2 was quantified for the different cell types and subcellular regions. Significant eNOS immunoreactivity was identified in the hair bundles, cuticular plates and the rest of the cytoplasm of the hair cells as well as in different subcellular regions of the supporting cells. Gold-labelled anti-nNOS antibodies stained mainly stereovilli and cuticular structures of hair cells and supporting cells, whereas the number of the immunoreactions in the remaining cytoplasm of both cell types was near the background level. The spatial co-localization of the two NOS isotypes in the same cell regions of hair cells and supporting cells was confirmed in double-labelling experiments. The immunocytochemical findings are suggestive of a redundant system in which one NOS isoform can (partially) replace the other. The different subcellular localization of the NOS isoforms may allow for isoform specific regulation of NOS activity by different Ca2+ currents at the subcellular level, underlining the importance of NO-regulated processes in neuroepithelia of the inner ear.

  1. Brian Eno Hollandi Festivali peakülaliseks

    Index Scriptorium Estoniae

    1999-01-01

    Inglise helilooja, videokunstnik ja muusikaprodutsent Brian Eno on 5.-26. juunini Amsterdamis toimuva avangardse Hollandi Festivali peakülaline, kelle videoinstallatsioon seatakse üles Stedelijki Muuseumis. Festivali programmist

  2. Labour Arrangements in Cassava Production in Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Abila, N.

    2012-01-01

    Full Text Available The study examined the effects of labour arrangements on the profitability of cassava enterprises in Oyo North Area of Oyo State, Nigeria. A multi-stage sampling technique was adopted for data collection, while data were analysed using descriptive statistics and budgetary analysis. The results show that the prevalent labour arrangements for cassava enterprises are: a combination of Family, Hired and Contract labour (38.9%; Family-Hired labour (27.8%; Family-Contract labour (31.1%. The gross margin per hectares across labour arrangements are N279481.99 (all-labour, N286044.24 (family-hired, N216940.10 (familycontract, and N235000.00 (family only. The returns on a naira invested on variable costs across different labour arrangements for cassava enterprises are N2.04 (all-labour, N3.66 (family-hired, N2.37 (familycontract, and N2.61 (family only. This implies that a unit (N1 variable cost in the various labour arrangements of all-labour, family/hired, family/contract and family only in cassava production will yield a marginal return of N3.04, N3.66, N2.37 and N2.61 respectively. Family-hired labour arrangement yields higher marginal return per unit of manday and one naira spent than all other arrangements. The study recommends among others the application of laboursaving technologies and an optimum combination of various labour arrangements to reduce the cost of labour used in cassava production.

  3. Application of a temperature selective heat storage tank to a solar system. Part 3. Solar heat collecting system; Ondo sentaku chikunetsuso no solar system eno tekiyo. 3. Shunetsu hoshiki ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Kamiya, Y. [Kanto Gakuin University, Yokohama (Japan); Kanayama, K. [Kitami Institute of Technology, Hokkaido (Japan)

    1996-10-27

    The tank system and tank-less system when the temperature selective heat storage tank is applied to a solar system were considered. In the tank system, the simulation shows that the annual supplementary heat consumption is reduced as the tank capacity becomes lower. The most suitable operating time set for determining the reference time is about five hours in winter and about nine hours in summer. The annual operating time is about 6.5 hours. In the tank-less system, the most suitable minimum flow rate per solar collector area of a heating medium in Tokyo and its districts is 10 L/hm{sup 2} for both three-step flow control and constant flow control. The tank-less system is slightly lower in annual supplementary heat consumption than the tank system. For the three-step flow control, a change in the annual supplementary heat consumption is lower than that in the minimum flow rate. For the constant flow control, however, the annual supplementary heat consumption rapidly increases when the flow rate more decreases than the optimum value. The number of pump start/stop counts for the three-step flow control is less than two times as high as for the constant flow control. 4 refs., 6 figs., 1 tab.

  4. Analisis Seismisitas dan Energi Gempabumi diKawasan Jalur Sesar OpakOyo Yogyakarta

    Directory of Open Access Journals (Sweden)

    Nugroho Budi Wibowo

    2017-11-01

    THE ANALYSIS OF SEISMICITY AND EARTHQUAKE ENERGY IN OPAK-OYA FAULT AREA YOGYAKARTA Earthquake occurred in the Region of Yogyakarta were caused by fault activities on the surface, such as Opak-Oyo Fault, Dengkeng Fault and faults in the Menoreh Hills and plate tectonic activities in South of Java. Opak-Oyo Fault activity are reflected from epicenter distribution and earthquake energy. This research aimed to analyze the earthquake energy which has epicenter around the Opak-Oyo Fault in spatial and temporal. Based on earthquake catalogue from 2008 – 2016, there were 66 earthquakes with 11 significant events. Epicenter distribution was reflected in seismicity map and for the tectonic parameter least square method was used. Seismicity map of Opak-Oyo Fault area showed that distribution of epicenter in the Eastern and Southeastern of fault line with the calculation result of b value using least square method is 0.4. In temporal, there is an increasing trend of events in 2008-2015 and declining trend of earthquake in 2015-2017. Earthquake energy that released in Opak-Oyo Fault area has varying value about 1×105.2 – 1×1012 joules. Meanwhile, the energy that associated with the existence of Opak Fault Lines has value between 1×105.2 – 1×109.6 joule with hypocenter of 10-20 km. The energy distribution has similar pattern with geological formations in that area.   Keyword : Opak-Oyo Fault, epicenter, seismicity, least square, energy.

  5. KAJIAN GEOMORFOLOGI UNTUK PERENCANAAN PENGGUNAAN LAHAN DI DAERAH ALIRAN SUNGAI OYO, GUNUNGKIDUL, DAERAH ISTIMEWA YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Karmono Mangunsukardjo

    2016-10-01

    Full Text Available ABSTRAK Penelitian ini bertujuan untuk mengetahui peranan geomorfologi dalam perencanaan penggunaan lahan di Daerah Aliran Sungai Oyo, Gunungkidul, Daerah Istimewa Yogyakarta. Arahan perencanaan penggunaan lahan didasarkan pada kelas kemampuan lahan dengan satuan medan sebagai satuan evaluasi dan acuan petanya. Satuan medan yang disusun atas satuan bentuklahan, lereng, dan tanah, sedangkan untuk penentuan kelas kemampuan lahannya ditambang dengan factor batu di permukaan, airtanah, dan genangan. Evaluasi kemampuan lahan dilakukan dengan cara matching antara karakteristik lahan dalam setiap satuan medan terhadap persyaratan kelas kemampuan lahan dengan menggunakan system informasi geografis (SIG. Hasil penelitian menunjukkan bahwa satuan medan yang disusun berdasarkan kerangka dasar geomorfologi mampu memberikan penilaian kemampuan lahan dan arahan penggunaan lahan. Satuan medan pegunungan structural-denudasional (SP, perbukitan structural-denudasional (SB, mempunyai kelas kemampuan lahan VI, sehingga tidak boleh dimanfaatkan dan seharusnya dijadikan lahan konservasi. Satuan medan lain yang mempunyai kelas kemampuan lahan IV seperti SBk, KD, KDt dapat dimanfaatkan untuk pertanian sangat terbatas. Satuan medan yang berkelas kemampuan lahan III adalah FT, SI, KDa, dan KLb yang memungkinkan untuk lahan pertanian terbatas. Erosi dan sifat tanah merupakan faktor kendala lahan pertanian pada satuan medan dengan kelas kemampuan lahan III.

  6. Application of the system free energy theory to the ferrite/pearlite transformation in hypo-eutectoid steel; Akyosekiko no ferrite pearlite hentai ni taisuru soshiki jiyu energy riron no oyo

    Energy Technology Data Exchange (ETDEWEB)

    Toda, Y.; Miyazaki, T. [Nagoya Institute of Technology, Nagoya (Japan)

    1998-12-01

    The kinetics of pearlite transformation in Fe-0.5mass%C steel has theoretically been investigated based on the system free energy theory proposed by us, by which the kinetics of phase transformation for each phase can be estimated according to its energy priority. The inter-lamellar spacing of pearlite is precisely predicted by the energy analysis, and the calculated time-temperature-transformation diagrams consist with the experimental ones. It is obvious that the system free energy theory is also effective for analyzing the lamellar structure produced by, e.g., the pearlite transformation in steel. (author)

  7. Endogenous urotensin II selectively modulates erectile function through eNOS.

    Directory of Open Access Journals (Sweden)

    Roberta d'Emmanuele di Villa Bianca

    Full Text Available BACKGROUND: Urotensin II (U-II is a cyclic peptide originally isolated from the neurosecretory system of the teleost fish and subsequently found in other species, including man. U-II was identified as the natural ligand of a G-protein coupled receptor, namely UT receptor. U-II and UT receptor are expressed in a variety of peripheral organs and especially in cardiovascular tissue. Recent evidence indicates the involvement of U-II/UT pathway in penile function in human, but the molecular mechanism is still unclear. On these bases the aim of this study is to investigate the mechanism(s of U-II-induced relaxation in human corpus cavernosum and its relationship with L-arginine/Nitric oxide (NO pathway. METHODOLOGY/PRINCIPAL FINDINGS: Human corpus cavernosum tissue was obtained following in male-to-female transsexuals undergoing surgical procedure for sex reassignment. Quantitative RT-PCR clearly demonstrated the U-II expression in human corpus cavernosum. U-II (0.1 nM-10 µM challenge in human corpus cavernosum induced a significant increase in NO production as revealed by fluorometric analysis. NO generation was coupled to a marked increase in the ratio eNOS phosphorilated/eNOS as determined by western blot analysis. A functional study in human corpus cavernosum strips was performed to asses eNOS involvement in U-II-induced relaxation by using a pharmacological modulation. Pre-treatment with both wortmannin or geldanamycinin (inhibitors of eNOS phosphorylation and heath shock protein 90 recruitment, respectively significantly reduced U-II-induced relaxation (0.1 nM-10 µM in human corpus cavernosum strips. Finally, a co-immunoprecipitation study demonstrated that UT receptor and eNOS co-immunoprecipitate following U-II challenge of human corpus cavernosum tissue. CONCLUSION/SIGNIFICANCE: U-II is endogenously synthesized and locally released in human corpus cavernosum. U-II elicited penile erection through eNOS activation. Thus, U-II/UT pathway may

  8. Cattle cruelty and risks of meat contamination at Akinyele cattle market and slaughter slab in Oyo State, Nigeria.

    Science.gov (United States)

    Adeyemo, Olanike K; Adeyemi, Isaac G; Awosanya, Emmanuel J

    2009-12-01

    Cattle transported to the government-run cattle market and slaughter slab in Akinyele, Oyo State, Nigeria on their final voyage are facing a disturbingly cruel, filthy and unsafe environment that is also raising the risk of contamination of meat sold for human consumption. This report gives a picture of what the cattle have to go through before they are slaughtered. This study also reveals cattle awaiting slaughter in abysmal health conditions, cows pulled with extreme force towards lairage and slaughter slab. Equally disturbing is the filthy situation inside the abattoir where the risk of contamination of meat is significant. Also, poor meat handling, transportation and sales practices subject meat to contamination leading to poor quality and exposure of human consumers to health risk. Development of hygienic slaughter slab operations, improved transportation system for both livestock and meat is therefore recommended; not only for Akinyele, but all abattoirs and slaughter slabs in Nigeria.

  9. LOČENO ZBIRANJE KOMUNALNIH ODPADKOV V KRAJEVNI SKUPNOSTI PREDOSLJE

    OpenAIRE

    Jovanović, Jaka

    2016-01-01

    Odpadki so stvari, s katerimi se dnevno srečujemo. Ljudje se vedno bolj zavedajo, kako pomembno je pravilno ločevanje posameznih odpadkov. Sistem ločenega zbiranja komunalnih odpadkov obstaja že nekaj let. Namen te diplomske naloge je bil preveriti, v kolikšni meri so krajani Predoselj sprejeli ločeno zbiranje komunalnih odpadkov.

  10. Upregulation of ANP and NPR-C mRNA in the kidney and heart of eNOS knockout mice.

    Science.gov (United States)

    Yuan, Kuichang; Kim, Sun Young; Oh, Young-Bin; Yu, Jiahua; Shah, Amin; Park, Byung Hyun; Kim, Suhn Hee

    2010-07-01

    The aim of the present studywas to examine the question of whether the atrial natriuretic peptide (ANP) system is altered by endothelial nitric-oxide synthase (eNOS). Male eNOS-deficient mice (eNOS-/-) and wild type control mice (eNOS+/+, C57B1/6J) were used. Blood pressure was measured in anesthetized mice by tail cuff plethysmography and renal function was measured. Expression of ANP, natriuretic peptide receptor (NPR)-A, NPR-C, and tonicity-responsive enhancer binding protein (TonEBP) mRNA was determined by real-time PCR. Localization of (125)I-ANP binding sites was measured using in vitro autoradiography. In eNOS-/- mice, systolic blood pressure increased and left ventricular hypertrophy was observed. Urine volume and osmolarity did not change. Expression of ANP markedly increased in the heart and kidney of eNOS-/- mice. Expression of NPR-A and NPR-C increased in the heart and tended to increase in the kidney of eNOS-/- mice. In the renal medulla in particular, increased expression of NPR-C was more prominent. Expression of TonEBP mRNA was markedly decreased in the renal medulla, but not in the renal cortex. Maximum binding capacity (B(max)) of ANP and C-ANP increased in the renal medulla in eNOS-/- mice. These results suggest that the eNOS-NO system may be partly involved in regulation of ANP, NPR-A, -C, and TonEBP mRNA expression in the kidney. Copyright 2010 Elsevier Inc. All rights reserved.

  11. The eNOS enhancer AVE 9488: a novel cardioprotectant against ischemia reperfusion injury.

    Science.gov (United States)

    Frantz, S; Adamek, A; Fraccarollo, D; Tillmanns, J; Widder, J D; Dienesch, C; Schäfer, A; Podolskaya, A; Held, M; Ruetten, H; Ertl, G; Bauersachs, J

    2009-11-01

    Nitric oxide (NO) is an important regulator of vascular and myocardial function. Cardiac ischemia/reperfusion injury is reduced in mice overexpressing endothelial NO synthase (eNOS) suggesting cardioprotection by eNOS. Novel pharmacological substances, so called eNOS enhancers, upregulate eNOS expression and thereby increase NO production. We tested the effects of the eNOS enhancer AVE 9488 on cardiac ischemia/reperfusion injury in vivo in mice. After treatment with the eNOS enhancer AVE 9488 (30 mg/kg/day) or placebo for one week mice underwent 30 min of coronary artery ligation and 24 h of reperfusion in vivo. Ischemia-reperfusion damage was significantly reduced in mice treated with the eNOS enhancer when compared to placebo treated mice (infarct/area at risk 65.4 +/- 4.1 vs. 36.9 +/- 4.0%, placebo vs. eNOS enhancer, P = 0.0002). The protective effect was blunted in eNOS knockout mice treated with the eNOS enhancer (infarct/area at risk 64.1 +/- 6.2%, eNOS knockout + eNOS enhancer vs. WT + eNOS enhancer, P = ns). Reactive oxygen species were significantly reduced in mice treated with the eNOS enhancer as indicated by significantly lower malondialdehyde-thiobarbituric acid levels (placebo vs. eNOS enhancer, 3.2 +/- 0.5 vs. 0.8 +/- 0.07 micromol/l, P = 0.0003). Thus pharmacological interventions addressed to increase eNOS-derived NO production constitute a promising therapeutic approach to prevent myocardial ischemia/reperfusion injury.

  12. Beneficial effects of exercise training after myocardial infarction require full eNOS expression.

    Science.gov (United States)

    de Waard, Monique C; van Haperen, Rien; Soullié, Thomas; Tempel, Dennie; de Crom, Rini; Duncker, Dirk J

    2010-06-01

    Exercise training attenuates left ventricular (LV) dysfunction after myocardial infarction (MI). It could be speculated that these effects of exercise are mediated by increased endothelial NO synthase (eNOS) activity. In the present study we tested the hypothesis that eNOS plays a critical role in the exercise-induced amelioration of LV dysfunction after MI. MI or sham was induced in eNOS(-/-), eNOS(+/-) and eNOS(+/+) mice. After 8 weeks of voluntary wheel running (approximately 7 km/day in all groups) or sedentary housing, global cardiac function was determined in vivo and (immuno)histochemistry was performed to assess cardiomyocyte size, fibrosis, capillary density and apoptosis in remote myocardium. At baseline eNOS(-/-) mice had higher mean aortic pressure compared to eNOS(+/-) and eNOS(+/+) mice, but had normal global cardiac function. MI resulted in marked LV remodeling, including cardiomyocyte hypertrophy and a reduction in capillary density, increased fibrosis and apoptosis, as well as LV systolic and diastolic dysfunction to the same extent in all genotypes. In eNOS(+/+) MI mice exercise abolished fibrosis and apoptosis in the remote myocardium, attenuated LV systolic dysfunction and ameliorated pulmonary congestion. These beneficial effects were lost in eNOS(+/-) and eNOS(-/-) mice, while LV systolic dysfunction and pulmonary congestion in eNOS(+/-) mice were exacerbated by exercise. In conclusion, the beneficial effects of exercise after MI on LV remodeling and dysfunction depend critically on endogenous eNOS. The observation that the lack of one eNOS allele is sufficient to negate all beneficial effects of exercise, strongly suggests that exercise depends on full eNOS expression. (c) 2010 Elsevier Ltd. All rights reserved.

  13. Preparation and applications of carbon film obtained by pulse-laser irradiation to polyimide; Polyimide eno pulse laser shosha ni yotte erareru tanso film to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Endo, M.; Naito, K.; Ishibe, S.; Ueda, M.; Kasai, T. [Shinshu University, Nagano (Japan); Oshida, K. [Nagano National College of Technology, Nagano (Japan)

    1997-05-20

    Described in this report is a novel effort for forming conductive paths by directly carbonizing a high-polymer film by laser beam irradiation. In the experiment, a polymer film is attached onto a movable X-Y stage, accommodated in a chamber, and then exposed to 10Hz pulsed YAG laser irradiation. A polyimide film is chosen for the experiment because it is easily cabonized in an ordinary pyrolytic process for the realization of high crystallinity. The carbon is then subjected to microscopic scrutiny for its structural details under the FE-SEM and TEM. It is found as the result that possibilities are high that the thus-obtained carbon may be used for the construction of practical circuits. It is found also that carbonization advances with the passage of irradiation time in a nitrogen atmosphere and that the carbon layer does not grow in thickness in the ordinary atmosphere where oxidation occurs simultaneously with carbonization. It is expected that this technique will be applied to the formation of highly conductive paths because it allows the easy and proper deposition of nickel onto the carbonized paths. 10 refs., 9 figs.

  14. Genetic algorithm based on virus theory of evolution for traveling salesman problem; Virus shinkaron ni motozuku identeki algorithm no junkai salesman mondai eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Kubota, N. [Osaka Inst. of Technology, Osaka (Japan); Fukuda, T. [Nagoya University, Nagoya (Japan)

    1998-05-31

    This paper deals with virus evolutionary genetic algorithm. The genetic algorithms (GAs) have been demonstrated its effectiveness in optimization problems in these days. In general, the GAs simulate the survival of fittest by natural selection and the heredity of the Darwin`s theory of evolution. However, some types of evolutionary hypotheses such as neutral theory of molecular evolution, Imanishi`s evolutionary theory, serial symbiosis theory, and virus theory of evolution, have been proposed in addition to the Darwinism. Virus theory of evolution is based on the view that the virus transduction is a key mechanism for transporting segments of DNA across species. This paper proposes genetic algorithm based on the virus theory of evolution (VE-GA), which has two types of populations: host population and virus population. The VE-GA is composed of genetic operators and virus operators such as reverse transcription and incorporation. The reverse transcription operator transcribes virus genes on the chromosome of host individual and the incorporation operator creates new genotype of virus from host individual. These operators by virus population make it possible to transmit segment of DNA between individuals in the host population. Therefore, the VE-GA realizes not only vertical but also horizontal propagation of genetic information. Further, the VE-GA is applied to the traveling salesman problem in order to show the effectiveness. 20 refs., 10 figs., 3 tabs.

  15. Application of photo-excitation reaction on titanium oxide thin film for control of wettability; Sanka chitann hakumakujo no hikari reiki hanno no nuresei seigyo eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, T.; Nakajima, A.; Hashimoto, K. [The Univ. of Tokyo, Tokyo (Japan); Takada, Y. [Kyushu Univ., Fukuoka (Japan)

    2000-03-31

    It is clarified that the photo-excitation hydrophilic reaction increasing wettability remarkably is induced by changing surface structure of titanium oxide radiated light. There are already many examples being in practical use of coating products applied hydrophilic reaction of titanium oxide surface such as drip-proof side millers for automobiles, self-cleaning building materials, and the like. When surface of titanium oxide having high activities for oxidisation and decomposition is coated organic materials and radiated light, wettability of surface changes as organic materials are decomposed. If it is possible to change wettability shaping pattern drastically by radiating light, the possibility of application for printing materials will be developed. After increasing contact angle by coating water and oil repellent on the titanium oxide thin film, images can be shaped by radiating light into pattern for changing surface of titanium oxide to be ultra hydrophilicity as decomposition of repellent. At that time, contact angle is 150 degree in water, 80 degree in oil, for not radiated aria, and is 0 degree in water and oil for radiated aria. Application for control technology of wettability keeps possibility of broader development to itself, not staying ability of self-cleaning and drip-proof. (NEDO)

  16. An Analysis of Rural Poverty in Oyo State: A principal Component ...

    African Journals Online (AJOL)

    The 2003/2004 NLSS data was used in this paper to consider the status of rural household poverty in Oyo State, Nigeria and to identify the relevant determinants using the. Principal component analysis (PCA) was employed to determine the important factors associated with rural household poverty. Household size ...

  17. Statistical Analysis of Poverty in Oyo-State: A Q -Approach

    African Journals Online (AJOL)

    PROF. OLIVER OSUAGWA

    2014-12-01

    Dec 1, 2014 ... Abstract. The limited scope of uni-dimensional money-metric approach of analyzing poverty based on either expenditure or income has made approaches that allow poverty to be studied at several dimensions a necessity. It is in the light of this, that this study was aimed at analyzing poverty in Oyo State ...

  18. Non-meritocratic Factors and the Recruitment Process in Oyo State ...

    African Journals Online (AJOL)

    NON-MERITOCRATIC FACTORS AND THE RECRUITMENT PROCESS IN. OYO STATE CIVIL SERVICE, NIGERIA the exploratory research design. It was also non-experimental and cross-sectional in nature. The exploratory research design was considered appropriate because we sought to explore the subject matter, ...

  19. Statistical Analysis of Poverty in Oyo-State: A Q 2 -Approach ...

    African Journals Online (AJOL)

    The limited scope of uni-dimensional money-metric approach of analyzing poverty based on either expenditure or income has made approaches that allow poverty to be studied at several dimensions a necessity. It is in the light of this, that this study was aimed at analyzing poverty in Oyo State using a Q2-approach.

  20. oral hygiene status of adolescents in a local government area of oyo ...

    African Journals Online (AJOL)

    User

    in maintaining good oral health of adolescents, it is often necessary to determine their oral hy- giene status from time to time with the aim of discovering possible risk to oral health prob- lems among them. This study thus aimed at assessing and describing the oral hygiene status of adolescents in Ibadan North (LGA) of Oyo.

  1. Non-meritocratic Factors and the Recruitment Process in Oyo State ...

    African Journals Online (AJOL)

    This study focuses on non-meritocratic factors that influence recruitment process. The main purpose was to investigate the factors that influence recruitment and selection in Oyo state civil service apart from merit, and ascertain perceived consequences of non-meritocracy in the recruitment process. The survey research ...

  2. Perceived effects of climate change on food crops production in Oyo ...

    African Journals Online (AJOL)

    The study assessed the perceived effects of climate change on food crops production in Oyo State. Multi stage sampling procedure was used in selecting 120 respondents for the study. Primary data was collected through interview schedule and it was analyzed using both descriptive and inferential statistics. Results reveal ...

  3. Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using 13 C metabolic flux analysis

    Directory of Open Access Journals (Sweden)

    Usui Yuki

    2012-06-01

    Full Text Available Abstract Background It has long been recognized that analyzing the behaviour of the complex intracellular biological networks is important for breeding industrially useful microorganisms. However, because of the complexity of these biological networks, it is currently not possible to obtain all the desired microorganisms. In this study, we constructed a system for analyzing the effect of gene expression perturbations on the behavior of biological networks in Escherichia coli. Specifically, we utilized 13 C metabolic flux analysis (13 C-MFA to analyze the effect of perturbations to the expression levels of pgi and eno genes encoding phosphoglucose isomerase and enolase, respectively on metabolic fluxes. Results We constructed gene expression-controllable E. coli strains using a single-copy mini F plasmid. Using the pgi expression-controllable strain, we found that the specific growth rate correlated with the pgi expression level. 13 C-MFA of this strain revealed that the fluxes for the pentose phosphate pathway and Entner-Doudoroff pathway decreased, as the pgi expression lelvel increased. In addition, the glyoxylate shunt became active when the pgi expression level was almost zero. Moreover, the flux for the glyoxylate shunt increased when the pgi expression level decreased, but was significantly reduced in the pgi-knockout cells. Comparatively, eno expression could not be decreased compared to the parent strain, but we found that increased eno expression resulted in a decreased specific growth rate. 13 C-MFA revealed that the metabolic flux distribution was not altered by an increased eno expression level, but the overall metabolic activity of the central metabolism decreased. Furthermore, to evaluate the impact of perturbed expression of pgi and eno genes on changes in metabolic fluxes in E. coli quantitatively, metabolic sensitivity analysis was performed. As a result, the perturbed expression of pgi gene had a great impact to the

  4. Application of a temperature selective storage tank solar system. Part 4. Fundamental experiment under a solar simulator; Ondo sentaku chikunetsuso no solar system eno tekiyo. 4. Solar simulator ni yoru kiso jikken

    Energy Technology Data Exchange (ETDEWEB)

    Kanayama, K.; Endo, N.; Baba, H.; Okamoto, A. [Kitami Institute of Technology, Hokkaido (Japan); Kamiya, Y. [Kanto Gakuin University, Yokohama (Japan)

    1996-10-27

    The storage tank is classified into a mixed type and stratified type. The stratified type is judged to be more advantageous from a viewpoint of the effective energy utilization. An experiment was made using a solar simulator to put the system, consisting of a vacuum double-glass tube collector and temperature selective storage tank, to practical use. The ejection position of the storage tank at the top is superior to that at the bottom, in the 60{degree}C layer of three layers (60, 40, and 20{degree}C). The ejection position hardly varies with the shape (straight or elbow) of an ejection port. When the temperature stratified layer is formed in two layers (40 {times} 2, 20{degree}C) to three layers (60, 40, and 20{degree}C), heat can be stably stored as the flow rate is higher. The stratified storage tank is inferior to the mixed storage tank in heat collection efficiency, but the specific exergy increases. By increasing the number of heat storage layers, the result of this experiment can also be applied to the linear temperature gradient layer obtained in the practical use. As a result of the above experiment, the basic data for an automated system design during practical application was obtained. 3 refs., 15 figs.

  5. Effect of exercise training on eNOS expression, NO production and oxygen metabolism in human placenta.

    Directory of Open Access Journals (Sweden)

    Robinson Ramírez-Vélez

    Full Text Available OBJECTIVE: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS, nitric oxide (NO production and oxygen metabolism in human placenta. METHODS: The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10 took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each using barbells (1-3 kg/exercise and low-to-medium resistance bands. The control group (n = 10 undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. RESULTS: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05. Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05. CONCLUSION: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on

  6. Arsenic, cadmium and neuron specific enolase (ENO2, γ-enolase) expression in breast cancer.

    Science.gov (United States)

    Soh, Maureen A; Garrett, Scott H; Somji, Seema; Dunlevy, Jane R; Zhou, Xu Dong; Sens, Mary Ann; Bathula, Chandra S; Allen, Christina; Sens, Donald A

    2011-11-18

    Neuron specific enolase (ENO2, γ-enolase) has been used as a biomarker to help identify neuroendocrine differentiation in breast cancer. The goal of the present study was to determine if ENO2 expression in the breast epithelial cell is influenced by the environmental pollutants, arsenite and cadmium. Acute and chronic exposure of MCF-10A cells to As+3 and Cd+2 sufficient to allow colony formation in soft agar, was used to determine if ENO2 expression was altered by these pollutants. It was shown that both As+3 and Cd+2 exposure caused significant increases in ENO2 expression under conditions of both acute and chronic exposure. In contrast, ENO1, the major glycolytic enolase in non-muscle and neuronal cells, was largely unaffected by exposure to either As+3 or Cd+2. Localization studies showed that ENO2 in the MCF-10A cells transformed by As+3 or Cd+2 had both a cytoplasmic and nuclear localization. In contrast, ENO1 was localized to the cytoplasm. ENO2 localized to the cytoplasm was found to co-localized with ENO1. The results are the first to show that ENO2 expression in breast epithelial cells is induced by acute and chronic exposure to As+3 or Cd+2. The findings also suggest a possible link between As+3 and Cd+2 exposure and neuroendocrine differentiation in tumors. Overall, the results suggest that ENO2 might be developed as a biomarker indicating acute and/or chronic environmental exposure of the breast epithelial cell to As+3 and Cd+2.

  7. Arsenic, cadmium and neuron specific enolase (ENO2, γ-enolase expression in breast cancer

    Directory of Open Access Journals (Sweden)

    Soh Maureen A

    2011-11-01

    Full Text Available Abstract Background Neuron specific enolase (ENO2, γ-enolase has been used as a biomarker to help identify neuroendocrine differentiation in breast cancer. The goal of the present study was to determine if ENO2 expression in the breast epithelial cell is influenced by the environmental pollutants, arsenite and cadmium. Acute and chronic exposure of MCF-10A cells to As+3 and Cd+2 sufficient to allow colony formation in soft agar, was used to determine if ENO2 expression was altered by these pollutants. Results It was shown that both As+3 and Cd+2 exposure caused significant increases in ENO2 expression under conditions of both acute and chronic exposure. In contrast, ENO1, the major glycolytic enolase in non-muscle and neuronal cells, was largely unaffected by exposure to either As+3 or Cd+2. Localization studies showed that ENO2 in the MCF-10A cells transformed by As+3 or Cd+2 had both a cytoplasmic and nuclear localization. In contrast, ENO1 was localized to the cytoplasm. ENO2 localized to the cytoplasm was found to co-localized with ENO1. Conclusion The results are the first to show that ENO2 expression in breast epithelial cells is induced by acute and chronic exposure to As+3 or Cd+2. The findings also suggest a possible link between As+3 and Cd+2 exposure and neuroendocrine differentiation in tumors. Overall, the results suggest that ENO2 might be developed as a biomarker indicating acute and/or chronic environmental exposure of the breast epithelial cell to As+3 and Cd+2.

  8. Association between nucleotide mutation of eNOS gene and serum ...

    African Journals Online (AJOL)

    Various mutation on endothelial nitric oxide synthase (eNOs) gene cause reduced production of NO, the expansion factor (VEF) and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C polymorphism of the gene or nucleotide mutation of eNOS gene in patients ...

  9. Stromal cell–derived factor 2 is critical for Hsp90-dependent eNOS activation

    Science.gov (United States)

    Siragusa, Mauro; Fröhlich, Florian; Park, Eon Joo; Schleicher, Michael; Walther, Tobias C.; Sessa, William C.

    2016-01-01

    Endothelial nitric oxide synthase (eNOS) catalyzes the conversion of l-arginine and molecular oxygen into l-citrulline and nitric oxide (NO), a gaseous second messenger that influences cardiovascular physiology and disease. Several mechanisms regulate eNOS activity and function, including phosphorylation at Ser and Thr residues and protein-protein interactions. Combining a tandem affinity purification approach and mass spectrometry, we identified stromal cell–derived factor 2 (SDF2) as a component of the eNOS macromolecular complex in endothelial cells. SDF2 knockdown impaired agonist-stimulated NO synthesis and decreased the phosphorylation of eNOS at Ser1177, a key event required for maximal activation of eNOS. Conversely, SDF2 overexpression dose-dependently increased NO synthesis through a mechanism involving Akt and calcium (induced with ionomycin), which increased the phosphorylation of Ser1177 in eNOS. NO synthesis by iNOS (inducible NOS) and nNOS (neuronal NOS) was also enhanced upon SDF2 overexpression. We found that SDF2 was a client protein of the chaperone protein Hsp90, interacting preferentially with the M domain of Hsp90, which is the same domain that binds to eNOS. In endothelial cells exposed to vascular endothelial growth factor (VEGF), SDF2 was required for the binding of Hsp90 and calmodulin to eNOS, resulting in eNOS phosphorylation and activation. Thus, our data describe a function for SDF2 as a component of the Hsp90-eNOS complex that is critical for signal transduction in endothelial cells. PMID:26286023

  10. An Eulerian method for computation of multimaterial impact with ENO shock-capturing and sharp interfaces

    CERN Document Server

    Udaykumar, H S; Belk, D M; Vanden, K J

    2003-01-01

    A technique is presented for the numerical simulation of high-speed multimaterial impact. Of particular interest is the interaction of solid impactors with targets. The computations are performed on a fixed Cartesian mesh by casting the equations governing material deformation in Eulerian conservation law form. The advantage of the Eulerian setting is the disconnection of the mesh from the boundary deformation allowing for large distortions of the interfaces. Eigenvalue analysis reveals that the system of equations is hyperbolic for the range of materials and impact velocities of interest. High-order accurate ENO shock-capturing schemes are used along with interface tracking techniques to evolve sharp immersed boundaries. The numerical technique is designed to tackle the following physical phenomena encountered during impact: (1) high velocities of impact leading to large deformations of the impactor as well as targets; (2) nonlinear wave-propagation and the development of shocks in the materials; (3) modelin...

  11. SLOŽENO KLIZANJE U DOLINI RJEČINE

    OpenAIRE

    Benac, Čedomir; Arbanas, Željko; Jardas, Branka; Kasapović, Saša; Jurak, Vladimir

    1999-01-01

    U radu su prikazani rezultati istraživanja prve faze složenog klizišta na sjeveroistočnoj padini doline Rječine između brane Valići i naselja Pašac. Bokovi doline oblikovani su u paleogenskom flišu i kvartarnim padinskim tvorevinama. Na vrhovima su zastupljene vapnenačke stijene tvoreći litice. Nastanak istražene kompleksne pojave klizišta predodređen je geološkom gradom i morfogenezom doline Rječine. To je složeno retrogresivno klizište, koje se počelo razvijati od stope prema vrhu kosine. N...

  12. FY 1997 report on the survey of potential impacts of enlarging ASEAN on political and economic systems in South East Asia; 1997 nendo chosa hokokusho (ASEAN kakudai no Higashi Asia no seiji keizai chitsujo eno eikyo chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This report surveys potential impacts of ASEAN (Association of South East Asian Nations) on inter-ASEAN affairs and its external relations when ASEAN will enlarge its members to include all nations in South East Asia, and thus fully represent the region. For this purpose, the survey was conducted on Vietnam, Myanmar, Laos and Cambodia, which joined in 1995, from the viewpoint of their economic and political system, and their relations with other member countries. The nature of ASEAN has gradually transformed, in which all the countries in the region have increased and internal economic issues have been tackled. It has an aim to stimulate inter-ASEAN trade and induce foreign direct investment into ASEAN as a whole by reducing import duties on intra-ASEAN trade. Underlying in these, new development is a concern about growing economic and military power of China. ASEAN solidarity will work an leverage against China should change toward worse, and ASEAN will function as a regional stabilization factor. ASEAN is needed for the stability of both in economic and political order in East Asia. Japan has to further promote its cooperation with ASEAN to help its solidarity as an association. 24 refs., 21 figs., 25 tabs.

  13. A Survey of Primary School Libraries to Determine the Availability and Adequacy of Services for Universal Basic Education (UBE in Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Belau Olatunde Gbadamosi

    2011-06-01

    Full Text Available Objective -- As a first step in gathering evidence, this study surveyed school libraries and examined the services those libraries provide in relation to the Universal Basic Education (UBE program at a primary level. The purpose of this paper is to explore these UBE factors in relation to school libraries in Oyo State, Nigeria.Methods -- A questionnaire was sent to the 33 schools which have a library and information centre staffed by a teacher-librarian, in order to examine library services and the views of those providing them. The questionnaire covers library space, categories of library resources, student activities during library visits, mobile library services, and the teacher-librarians’ perception of the adequacy of the collection and the challenges in the use of library.Results -- Questionnaires were returned by 30 teacher-librarians out of 33, a response rate of 91%. Although pupils across each local government area have the chance to visit school libraries, the system of one short visit per week is inadequate for developing a universal interest in reading and in study skills. Staff responsible for the libraries consider many of the resources to be inadequate or outdated and stock is depleted as pupils borrow books, leaving fewer for those who follow.Conclusions -- As a first step to implementing evidence based practice, this paper describes the primary school library system in Nigeria and provides evidence on how it operates in Oyo State. Teacher-librarians who staff the libraries confirm the inadequacies in the system. They perceive collections to be inadequate and confirm that some pupils in the state have only limited access to library resources. Respondents also believe that they need further training, particularly in computer and information literacy.

  14. Analysis of Child Labour among Rural Household of Oyo State, Nigeria

    OpenAIRE

    Alao Bashir Idowu; Olasore Abiodun Amos; Aremu Adeola Olabisi (Mrs.)

    2014-01-01

    This study is on analysis of child labour determinants among the farming households of Oyo State, Nigeria. Data for the study were obtained from 120 respondents from the target group using a three-stage sampling procedure and analyzed using descriptive statistics and logistic model. Results showed that 85% of the respondents are male. The mean age of the respondent is 58 years 74.16% of them are married. Religions of the respondent are 40%, 53.33%, and 6.67% for Christianity, Islam and tradit...

  15. Normal and high eNOS levels are detrimental in both mild and severe cardiac pressure-overload.

    Science.gov (United States)

    van Deel, Elza D; Octavia, Yanti; de Boer, Martine; Juni, Rio P; Tempel, Dennie; van Haperen, Rien; de Crom, Rini; Moens, An L; Merkus, Daphne; Duncker, Dirk J

    2015-11-01

    Nitric oxide (NO) produced by endothelial NO synthase (eNOS) exerts beneficial effects in a variety of cardiovascular disease states. Studies on the benefit of eNOS activity in pressure-overload cardiac hypertrophy and dysfunction produced by aortic stenosis are equivocal, which may be due to different expression levels of eNOS or different severities of pressure-overload. Consequently, we investigated the effects of eNOS-expression level on cardiac hypertrophy and dysfunction produced by mild or severe pressure-overload. To unravel the impact of eNOS on pressure-overload cardiac dysfunction we subjected eNOS deficient, wildtype and eNOS overexpressing transgenic (eNOS-Tg) mice to 8weeks of mild or severe transverse aortic constriction (TAC) and studied cardiac geometry and function at the whole organ and tissue level. In both mild and severe TAC, lack of eNOS ameliorated, whereas eNOS overexpression aggravated, TAC-induced cardiac remodeling and dysfunction. Moreover, the detrimental effects of eNOS in severe TAC were associated with aggravation of TAC-induced NOS-dependent oxidative stress and by further elevation of eNOS monomer levels, consistent with enhanced eNOS uncoupling. In the presence of TAC, scavenging of reactive oxygen species with N-acetylcysteine reduced eNOS S-glutathionylation, eNOS monomer and NOS-dependent superoxide levels in eNOS-Tg mice to wildtype levels. Accordingly, N-acetylcysteine improved cardiac function in eNOS-Tg but not in wildtype mice with TAC. In conclusion, independent of the severity of TAC, eNOS aggravates cardiac remodeling and dysfunction, which appears due to TAC-induced eNOS uncoupling and superoxide production. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. A2B adenosine receptor contributes to penile erection via PI3K/AKT signaling cascade-mediated eNOS activation

    Science.gov (United States)

    Wen, Jiaming; Grenz, Almut; Zhang, Yujin; Dai, Yingbo; Kellems, Rodney E.; Blackburn, Michael R.; Eltzschig, Holger K.; Xia, Yang

    2011-01-01

    Normal penile erection is under the control of multiple factors and signaling pathways. Although adenosine signaling is implicated in normal and abnormal penile erection, the exact role and the underlying mechanism for adenosine signaling in penile physiology remain elusive. Here we report that shear stress leads to increased adenosine release from endothelial cells. Subsequently, we determined that ecto-5′-nucleotidase (CD73) is a key enzyme required for the production of elevated adenosine from ATP released by shear-stressed endothelial cells. Mechanistically, we demonstrate that shear stress-mediated elevated adenosine functions through the adenosine A2B receptor (A2BR) to activate the PI3K/AKT signaling cascade and subsequent increased endothelial nitric oxide synthase (eNOS) phosphorylation. These in vitro studies led us to discover further that adenosine was induced during sustained penile erection and contributes to PI3K/AKT activation and subsequent eNOS phosphorylation via A2BR signaling in intact animal. Finally, we demonstrate that lowering adenosine in wild-type mice or genetic deletion of A2BR in mutant mice significantly attenuated PI3K/AKT activation, eNOS phosphorylation, and subsequent impaired penile erection featured with the reduction of ratio of maximal intracavernosal pressure to systemic arterial pressure from 0.49 ± 0.03 to 0.41 ± 0.05 and 0.38 ± 0.04, respectively (both Ppenile erection via PI3K/AKT-dependent eNOS activation. These studies suggest that this signaling pathway may be a novel therapeutic target for erectile disorders.—Wen, J., Grenz, A., Zhang, Y., Dai, Y., Kellems, R. E., Blackburn, M. R., Eltzschig, H. K., Xia, Y. A2B adenosine receptor contributes to penile erection via PI3K/AKT signaling cascade-mediated eNOS activation. PMID:21566208

  17. Esquemas de quinto orden Weighted Power‐ENO para ecuaciones de Hamilton‐Jacobi

    OpenAIRE

    Martínez Moreno, Salvador

    2013-01-01

    En este trabajo han sido diseñados una clase de esquemas Weighted Power‐ENO empleados para aproximar la solución viscosa de las ecuaciones HJ. La idea esencial de este tipo de reconstrucción Power‐ENO es la aplicación a una clase de limitadores extendidos a diferencias de segundo orden en las reconstrucciones ENO clásicas de tercer orden, con el objetivo de mejorar las soluciones cerca de las discontinuidades. La estrategia de pesado basada en apropiados indicadores de suavidad...

  18. FY 1998 result report on development of superconductive power application technologies. Pt. 1. Research and development of superconductive wire materials / Research of a total system / Research and development of a freezing system / Demonstration tests; 1998 nendo chodendo denryoku oyo gijutsu kaihatsu. 1. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total system no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Superconductive technologies are introduced into electric power devices for attempts of achieving higher stabilization, density and efficiency, as well as size and weight reduction and improvement in performance of the devices. The project has been worked on since fiscal 1998 as part of the New Sunshine Project. Fiscal 1998 being the eleventh year has taken the following subjects as the research promotion policies: establishment of plans targeted at accomplishment of the goals of the project; adequate and reliable implementation of verification of technological assignments; and steady and efficient demonstration tests. Subsequent to the previous year during which site demonstration tests were completed on a low-speed responsive model machine, the site demonstration test has begun on the ultra high-speed responsive model machine as the final stage of the project. The ultra high-speed responsive model machine was coupled with a freezing system and a load synchronizing machine, and different kinds of test were carried out where good results were obtained. Researches were conducted on characteristics improvement and device element technologies aimed at achieving the practical application level by utilizing the respective features of AC metal-based wires and oxide-based wires, where sound results were obtained. Also in an improved freezing system, valuable data were attained as part of establishing the basic technologies for a superconductive power generation system. (NEDO)

  19. Activities and influence of veterinary drug marketers on antimicrobial usage in livestock production in Oyo and Kaduna States, Nigeria

    OpenAIRE

    Ojo, Olufemi Ernest; Awoyomi, Olajoju Jokotola; Fabusoro, Eniola; Dipeolu, Morenike Atinuke

    2017-01-01

    Antimicrobial usage in animals contributes to the emergence of antimicrobial resistant bacterial strains. Investigations were carried out on how the characteristics, knowledge, attitude and practices of antimicrobial marketers influenced antimicrobials usage in animal production in Oyo and Kaduna States, Nigeria. Focus group discussions, in-depth interviews and structured questionnaires were used to gather information about the characteristics and activities of antimicrobial marketers. Overal...

  20. Effects of Training Programme on HIV/AIDS Prevention among Primary Health Care Workers in Oyo State, Nigeria

    Science.gov (United States)

    Ajuwon, Ademola; Funmilayo, Fawole; Oladepo, Oladimeji; Osungbade, Kayode; Asuzu, Michael

    2008-01-01

    Purpose: The purpose of this paper is to train primary health care workers to be trainers and implementers of community-based AIDS prevention activities in Oyo State, Nigeria, by describing an evaluation of the project. Design/methodology/approach: A total of 148 primary health care workers recruited from the 33 local government areas (LGA) of the…

  1. Awareness of Climate Change and Sustainable Development among Undergraduates from Two Selected Universities in Oyo State, Nigeria

    Science.gov (United States)

    Agboola, Omowunmi Sola; Emmanuel, Michael

    2016-01-01

    This study investigated awareness of climate change and sustainable development among undergraduates in two universities: University of Ibadan, Ibadan and Ladoke Akintola University of Technology, Ogbomoso in Oyo State of Nigeria. This was aimed at increasing the knowledge of undergraduates on climate change and sustainable development. The study…

  2. Achievement report for fiscal 1996 on the research and development of superconductor technology to power generation. Pt. 1. Research and development of superconducting wire, generator, total system, and refrigeration system; and verification test; 1996 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 1. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total system no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    In the research and development of superconducting wires, studies are conducted to increase the current capacity of NbTi and Nb{sub 3}Sn metal wires and to improve their properties, and to increase the current capacity of oxide materials and improve their performance making full use of the features of each manufacturing method. In the development of superconducting generators, a slow excitation response type is tested for verification, and a good result is attained; and a quick excitation response type is tested for field winding static excitation, and good performance is exhibited. Using the results so far achieved, the 200,000kW class pilot machine concept design is reviewed. In the study of total systems, feasibility is studied of a quench test for the 70,000kW class machine through simulation analyses, etc. In the development of refrigeration systems, efforts are exerted to improve on the conventional type in terms of reliability and to further improve on the improved version in terms of performance and space-saving feature. One of the endeavors involves the development of a He Brayton cycle turbine driven compressor. A multilayer cylindrical rotor is verified in terms of functions, characteristics, reliability and durability, and various data are collected toward the development of a pilot machine. (NEDO)

  3. Activation of Endothelial Nitric Oxide (eNOS Occurs through Different Membrane Domains in Endothelial Cells.

    Directory of Open Access Journals (Sweden)

    Jason Tran

    Full Text Available Endothelial cells respond to a large range of stimuli including circulating lipoproteins, growth factors and changes in haemodynamic mechanical forces to regulate the activity of endothelial nitric oxide synthase (eNOS and maintain blood pressure. While many signalling pathways have been mapped, the identities of membrane domains through which these signals are transmitted are less well characterized. Here, we manipulated bovine aortic endothelial cells (BAEC with cholesterol and the oxysterol 7-ketocholesterol (7KC. Using a range of microscopy techniques including confocal, 2-photon, super-resolution and electron microscopy, we found that sterol enrichment had differential effects on eNOS and caveolin-1 (Cav1 colocalisation, membrane order of the plasma membrane, caveolae numbers and Cav1 clustering. We found a correlation between cholesterol-induced condensation of the plasma membrane and enhanced high density lipoprotein (HDL-induced eNOS activity and phosphorylation suggesting that cholesterol domains, but not individual caveolae, mediate HDL stimulation of eNOS. Vascular endothelial growth factor (VEGF-induced and shear stress-induced eNOS activity was relatively independent of membrane order and may be predominantly controlled by the number of caveolae on the cell surface. Taken together, our data suggest that signals that activate and phosphorylate eNOS are transmitted through distinct membrane domains in endothelial cells.

  4. Activation of Endothelial Nitric Oxide (eNOS) Occurs through Different Membrane Domains in Endothelial Cells.

    Science.gov (United States)

    Tran, Jason; Magenau, Astrid; Rodriguez, Macarena; Rentero, Carles; Royo, Teresa; Enrich, Carlos; Thomas, Shane R; Grewal, Thomas; Gaus, Katharina

    2016-01-01

    Endothelial cells respond to a large range of stimuli including circulating lipoproteins, growth factors and changes in haemodynamic mechanical forces to regulate the activity of endothelial nitric oxide synthase (eNOS) and maintain blood pressure. While many signalling pathways have been mapped, the identities of membrane domains through which these signals are transmitted are less well characterized. Here, we manipulated bovine aortic endothelial cells (BAEC) with cholesterol and the oxysterol 7-ketocholesterol (7KC). Using a range of microscopy techniques including confocal, 2-photon, super-resolution and electron microscopy, we found that sterol enrichment had differential effects on eNOS and caveolin-1 (Cav1) colocalisation, membrane order of the plasma membrane, caveolae numbers and Cav1 clustering. We found a correlation between cholesterol-induced condensation of the plasma membrane and enhanced high density lipoprotein (HDL)-induced eNOS activity and phosphorylation suggesting that cholesterol domains, but not individual caveolae, mediate HDL stimulation of eNOS. Vascular endothelial growth factor (VEGF)-induced and shear stress-induced eNOS activity was relatively independent of membrane order and may be predominantly controlled by the number of caveolae on the cell surface. Taken together, our data suggest that signals that activate and phosphorylate eNOS are transmitted through distinct membrane domains in endothelial cells.

  5. De novo lipogenesis maintains vascular homeostasis through endothelial nitric-oxide synthase (eNOS) palmitoylation.

    Science.gov (United States)

    Wei, Xiaochao; Schneider, Jochen G; Shenouda, Sherene M; Lee, Ada; Towler, Dwight A; Chakravarthy, Manu V; Vita, Joseph A; Semenkovich, Clay F

    2011-01-28

    Endothelial dysfunction leads to lethal vascular complications in diabetes and related metabolic disorders. Here, we demonstrate that de novo lipogenesis, an insulin-dependent process driven by the multifunctional enzyme fatty-acid synthase (FAS), maintains endothelial function by targeting endothelial nitric-oxide synthase (eNOS) to the plasma membrane. In mice with endothelial inactivation of FAS (FASTie mice), eNOS membrane content and activity were decreased. eNOS and FAS were physically associated; eNOS palmitoylation was decreased in FAS-deficient cells, and incorporation of labeled carbon into eNOS-associated palmitate was FAS-dependent. FASTie mice manifested a proinflammatory state reflected as increases in vascular permeability, endothelial inflammatory markers, leukocyte migration, and susceptibility to LPS-induced death that was reversed with an NO donor. FAS-deficient endothelial cells showed deficient migratory capacity, and angiogenesis was decreased in FASTie mice subjected to hindlimb ischemia. Insulin induced FAS in endothelial cells freshly isolated from humans, and eNOS palmitoylation was decreased in mice with insulin-deficient or insulin-resistant diabetes. Thus, disrupting eNOS bioavailability through impaired lipogenesis identifies a novel mechanism coordinating nutritional status and tissue repair that may contribute to diabetic vascular disease.

  6. Contributions of Perfectionism and Social Support to the Prediction of Work-Family Conflict among Women Academics in Oyo State, Nigeria

    National Research Council Canada - National Science Library

    Samuel Toyin Akanbi

    2016-01-01

    ...) among women academics. A correlational method was employed for the study, one hundred and sixty-eight women academics in tertiary institutions in Oyo State were engaged in the study through a purposive random sampling technique...

  7. Food Consumption Pattern in Ogbomoso Metropolis of Oyo State ...

    African Journals Online (AJOL)

    The study shed light on food consumption pattern in Ogbomoso Metropolis using Almost Ideal Demand System. Information on different classes of food consumed by the household was obtained using a multistage random technique. The result showed that demand for root and tubers and vegetables are elastic than ...

  8. Evaluation of Protective Immune Responses Induced by Recombinant TrxLp and ENO2 Proteins against Toxoplasma gondii Infection in BALB/c Mice

    Directory of Open Access Journals (Sweden)

    Meng Wang

    2016-01-01

    Full Text Available Toxoplasma gondii is an obligate intracellular parasitic protozoan that can infect almost all species of warm-blooded animals. As any chemical-based drugs could not act against the tissue cyst stage of T. gondii, vaccination may be one of the ideal control strategies. In the present study, two new vaccine candidates, named TgENO2 and TgTrxLp, were purified from Escherichia coli with pET-30a(+ expression system and then were injected into BALB/c mice to evaluate the protective efficacy against acute and chronic toxoplasmosis. The results showed that both the recombinant proteins, either alone or in combination, could elicit strong humoral and cellular immune responses with a higher level of IgG antibodies, IFN-γ, IL-2, CD4+, and CD8+ T cells as compared to those in mice from control groups. After acute challenge with tachyzoites of the GJS strain, mice immunized with rTgTrxLp (8±2.77 d, rTgENO2 (7.4±1.81 d, and rTgTrxLp + rTgENO2 (8.38±4.57 d proteins showed significantly longer survival time than those that received Freund’s adjuvant (6.78±2.08 d and PBS (6.38±4.65 d (χ2 = 9.687, df = 4, P=0.046. The protective immunity of rTgTrxLp, rTgENO2, and rTgTrxLp + rTgENO2 proteins against chronic T. gondii infection showed 69.77%, 58.14%, and 20.93% brain cyst reduction as compared to mice that received PBS. The present study suggested that both TgENO2 and TgTrxLp were potential candidates for the development of multicomponent vaccines against toxoplasmosis.

  9. Determinants and perception of cardiovascular risk factors among secondary school teachers in Oyo state Nigeria.

    Science.gov (United States)

    Familoni, I F; Familoni, O B

    2011-12-01

    Cardiovascular diseases (CVD) are common. They constitute an increasing cause of morbidity and mortality. Knowledge of the risk factors may lead to attitudinal change with consequent reduction in prevalence. Secondary school teachers constitute a large literate workforce that has direct influence on students and indirectly on their parents and guardians. The aim of this study is to investigate the knowledge of cardiovascular risk factors among secondary school teachers in Oyo State, Nigeria and also the determinants of such knowledge. Three hundred and fifty eight secondary school teachers from the Akinyele local government of Oyo State Nigeria completed a questionnaire seeking such information as which diseases constitute CVD and also identify risk factors for CVD. The determinants of such knowledge were investigated by the log likehood ratio using logistic regression. Two hundred and fifteen civil servants matched for age, sex and qualifications were enrolled as controls. Of the 358 teachers, 12.3% were current smokers, 32.1% drank alcohol. More of the civil servant controls patronized fast food joints. Over 80% of the teachers performed exercise regularly, majority being 'walking' (66.2%). Hypertension (84.5%) and heart attack (87.6%) were the most correctly identified CVD. The least correctly identified was peripheral vascular disease (18.6%). The longer the years of teaching, specialization in pure science and being male the more likely the knowledge of sedentary living as a risk factor. Other variables that reached statistical significance include knowledge of stress, smoking and advanced age. Generally the knowledge of the teachers is inadequate about CVD and the risk factors. Qualification in pure science and years of teaching did not radically affect this knowledge. The knowledge base of the teachers needs to be improved.

  10. eNOS Uncoupling, A Therapeutic Target For Ischemic Foot of Diabetic Rat.

    Science.gov (United States)

    Gao, Ling; Yu, Ailin; Liu, Jingcheng; Ma, Lina; Li, Jing

    2018-02-01

    To determine the relationship between eNOS uncoupling and diabetic ischemic foot and whether reversing eNOS uncoupling by Dihydrofolate reductase (DHFR) transfection or folic acid (FA) supplementation can be beneficiary in diabetic ischemic foot. The bilateral common iliac artery of diabetic rats were ligated to establish the diabetic ischemic foot animal model. DHFR transfection was implemented via femoral artery and muscle injection of in vivo transfection reagent mixture (GenEscortIII) every 4 days during the 2 weeks intervention. The color doppler flow imaging (CDFI) of femoral artery for RI measurement, triceps and quadriceps structure and histology, eNOS coupling status, DHFR expression level, superoxide, peroxynitrite (ONOO- ) and nitric oxide (NO) production in the presence or absence of L-NAME (eNOS inhibitor) were examined among wild type rats (WT), diabetic sham rats (DM), rats of diabetic ischemic foot (DF) or DF with DHFR transfection (DFT) or DF with FA supplementation (DFF). Dihydroethidium (DHE) fluorescence, as an index of superoxide production was enhanced in the femoral arteries of diabetic rats and even more in those of ischemic foot from diabetic rats. However, the DHE fluorescence was diminished in the presence of L-NAME suggesting eNOS uncoupling is the source of superoxide overproduction which further led to increased peroxynitrite production and decreased NO . bioavailability. Subsequently, the hind limb muscle became atrophic and the local collateral circulation was defective due to endothelial dysfunction related to eNOS uncoupling. However, all of the above and hemodynamic index (RI) of femoral artery were resumed via restoration of DHFR protein expression by folic acid treatment or DHFR transfection. eNOS uncoupling is involved in diabetic ischemic foot due to DHFR suppression. DHFR restoration can reverse eNOS uncoupling and resume the endothelial dysfunction and pathological changes (increased vasculature resistance, hind limb muscle

  11. "Non alcoholic fatty liver disease and eNOS dysfunction in humans".

    Science.gov (United States)

    Persico, Marcello; Masarone, Mario; Damato, Antonio; Ambrosio, Mariateresa; Federico, Alessandro; Rosato, Valerio; Bucci, Tommaso; Carrizzo, Albino; Vecchione, Carmine

    2017-03-07

    NAFLD is associated to Insulin Resistance (IR). IR is responsible for Endothelial Dysfunction (ED) through the impairment of eNOS function. Although eNOS derangement has been demonstrated in experimental models, no studies have directly shown that eNOS dysfunction is associated with NAFLD in humans. The aim of this study is to investigate eNOS function in NAFLD patients. Fifty-four NAFLD patients were consecutively enrolled. All patients underwent clinical and laboratory evaluation and liver biopsy. Patients were divided into two groups by the presence of NAFL or NASH. We measured vascular reactivity induced by patients' platelets on isolated mice aorta rings. Immunoblot assays for platelet-derived phosphorylated-eNOS (p-eNOS) and immunohistochemistry for hepatic p-eNOS have been performed to evaluate eNOS function in platelets and liver specimens. Flow-mediated-dilation (FMD) was also performed. Data were compared with healthy controls. Twenty-one (38, 8%) patients had NAFL and 33 (61, 7%) NASH. No differences were found between groups and controls except for HOMA and insulin (p liver (p liver tissue didn't match with FMD.

  12. Librarian-initiated HIV/AIDS prevention intervention program outcome in rural communities in Oyo State, Nigeria.

    Science.gov (United States)

    Ajuwon, G A; Komolafe-Opadeji, H O; Ikhizama, B

    2013-01-01

    The objective of this study was to meet the HIV/AIDS information and service needs of citizens living in selected rural, underserved communities in Oyo State, Nigeria. This was a librarian-initiated intervention program (pre-post) study of heads of rural households in Oyo State. A questionnaire was used for pre- and post-intervention assessment. The education covered knowledge about HIV/AIDS, routes of transmission, prevention strategies, and attitude toward persons living with HIV. It increased participants' knowledge about AIDS and improved attitude toward those living with HIV. Provision and dissemination of information on HIV/AIDS through librarians to rural settlers is an important prevention strategy and librarians can make major contributions.

  13. Folic acid modulates eNOS activity via effects on posttranslational modifications and protein–protein interactions☆

    Science.gov (United States)

    Taylor, Sarah Y.; Dixon, Hannah M.; Yoganayagam, Shobana; Price, Natalie; Lang, Derek

    2013-01-01

    Folic acid enhances endothelial function and improves outcome in primary prevention of cardiovascular disease. The exact intracellular signalling mechanisms involved remain elusive and were therefore the subject of this study. Particular focus was placed on folic acid-induced changes in posttranslational modifications of endothelial nitric oxide synthase (eNOS). Cultured endothelial cells were exposed to folic acid in the absence or presence of phosphatidylinositol-3' kinase/Akt (PI3K/Akt) inhibitors. The phosphorylation status of eNOS was determined via western blotting. The activities of eNOS and PI3K/Akt were evaluated. The interaction of eNOS with caveolin-1, Heat-Shock Protein 90 and calmodulin was studied using co-immunoprecipitation. Intracellular localisation of eNOS was investigated using sucrose gradient centrifugation and confocal microscopy. Folic acid promoted eNOS dephosphorylation at negative regulatory sites, and increased phosphorylation at positive regulatory sites. Modulation of phosphorylation status was concomitant with increased cGMP concentrations, and PI3K/Akt activity. Inhibition of PI3K/Akt revealed specific roles for this kinase pathway in folic acid-mediated eNOS phosphorylation. Regulatory protein and eNOS protein associations were altered in favour of a positive regulatory effect in the absence of bulk changes in intracellular eNOS localisation. Folic acid-mediated eNOS activation involves the modulation of eNOS phosphorylation status at multiple residues and positive changes in important protein–protein interactions. Such intracellular mechanisms may in part explain improvements in clinical vascular outcome following folic acid treatment. PMID:23796957

  14. Post-translational regulation of endothelial nitric oxide synthase (eNOS) by estrogens in the rat vagina.

    Science.gov (United States)

    Musicki, Biljana; Liu, Tongyun; Strong, Travis D; Lagoda, Gwen A; Bivalacqua, Trinity J; Burnett, Arthur L

    2010-05-01

    Estrogens control vaginal blood flow during female sexual arousal mostly through nitric oxide (NO). Although vascular effects of estrogens are attributed to an increase in endothelial NO production, the mechanisms of endothelial NO synthase (eNOS) regulation by estrogens in the vagina are largely unknown. Our hypothesis was that estrogens regulate eNOS post-translationally in the vagina, providing a mechanism to affect NO bioavailability without changes in eNOS protein expression. We measured eNOS phosphorylation and eNOS interaction with caveolin-1 and heat shock protein 90 (HSP90) in the distal and proximal vagina of female rats at diestrus, 7 days after ovariectomy and 2 days after replacement of ovariectomized rats with estradiol-17beta (15 microg). Molecular mechanisms of eNOS regulation by estrogen in the rat vagina. We localized phospho-eNOS (Ser-1177) immunohistochemically to the endothelium lining blood vessels and vaginal sinusoids. Estrogen withdrawal decreased phosphorylation of eNOS on its positive regulatory site (Ser-1177) and increased eNOS binding to its negative regulator caveolin-1 (without affecting eNOS/HSP90 interaction), and they were both normalized by estradiol replacement. Protein expressions of phosphorylated Akt (protein kinase B) and extracellular signal-regulated protein kinase 1/2 (ERK1/2) were not affected by estrogen status, suggesting that the effect of estrogens on eNOS (Ser-1177) phosphorylation was not mediated by activated AKT or ERK1/2. eNOS phosphorylation on its negative regulatory site (Ser-114) was increased in the vagina by estrogen withdrawal and normalized by estradiol replacement, implying that the maintenance of low phosphorylation of eNOS on this site by estradiol may limit eNOS interaction with caveolin-1 and preserve the enzyme's activity. Total eNOS, inducible NOS, caveolin-1, and HSP90 protein expressions were not affected by ovariectomy or estradiol replacement in the distal or proximal vagina. These results

  15. Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression

    Directory of Open Access Journals (Sweden)

    Xiong Yuanzhu

    2008-09-01

    Full Text Available Abstract In this study, a full-length cDNA of the porcine ENO3 gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of ENO3 is highly expressed in liver and lung, while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of ENO3 is different between Yorkshire and Meishan pig breeds. Furthermore, real-time polymerase chain reaction revealed that, in Yorkshire pigs, skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover, expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2, with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the ENO3 gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage, average backfat thickness, meat marbling and intramuscular fat in two different populations.

  16. Zika virus infections in Nigeria: virological and seroepidemiological investigations in Oyo State.

    Science.gov (United States)

    Fagbami, A H

    1979-10-01

    A study of Zika virus infections was carried out in four communities in Oyo State, Nigeria. Virus isolation studies between 1971 and 1975 yielded two virus isolations from human cases of mild febrile illness. Haemagglutination-inhibition tests revealed a high prevalence of antibodies to Zika and three other flaviviruses used. The percentages of positive sera were as follows: Zika (31%), Yellow fever (50%), West Nile (46%), and Wesselsbron (59%). Neutralization tests showed that 40% of Nigerians had Zika virus neutralizing antibody. Fifty per cent of zika virus immune persons had neutralizing antibody to Zika alone or to Zika and one other flavivirus. A total of 121 sera had antibody to Zika virus; of these 48 (40%) also showed antibody to two other flaviviruses, and 12 (10%) had antibodies to three or more other viruses. The percentage of neutralizing antibodies to other flaviviruses in Zika virus immune sera was 81% to Dengue type 1, 58% to Yellow fever, 7% to Wesselsbron, 6% to West Nile and 3% to Uganda S.

  17. Recreation as a Complementary Capacity Building Strategy among Oyo State Civil Servants

    Directory of Open Access Journals (Sweden)

    Babatunde Samson OLUSOLA

    2014-06-01

    Full Text Available Meaningful capacity building should essentially include promotion and maintenance of health of workers. The nature of office responsibilities, if car e is not taken easily pre disposes workers to sedent ary , aetiology of heart diseases capable of promoting social, physical and mental health problems with adverse consequences on workers‟ productivity. The study focused complementary role of recreation in c apacity building among state civil servants of Oyo State in order to enhance, sustain and maintain their health. 2800 participants involving males and females drawn from 20 Ministries on equal basis, through randomization were used for the study. Self - deve loped questionnaire , validated for construct and content assurance w ere employed to collect data which were analysed with chi - square (X 2 inferential statistics at 0.05 alpha level. Based on the conclusions, recommendations were proffered on strategies to promote recreation among workers with the provision of recreational facilities at secretariats as well as int roduction of mandatory monthly „ walk for fitness ‟ by every rank and file of workers under the leadership of recreation experts.

  18. POVERTY AMONG CASSAVA FARMERS IN OYO STATE, NIGERIA – CHARACTERISTICS AND DRIVERS

    Directory of Open Access Journals (Sweden)

    Raphael Babatunde

    2016-09-01

    Full Text Available The signifi cance of rural poverty is underscored by the fact that a high percentage of the national population resides in the rural areas, is poor, and dependent on agriculture. The aim of this study is therefore to analyse the determinants of poverty status among cassava growing households in Oyo State, Nigeria. The specifi c objectives were to describe the socio-economic characteristics of the respondents, analyse the determinants of poverty status among the respondents, and examine the poverty profi le of the respondents based on their income class. Descriptive statistics, Probit Regression analysis and Foster-Greer-Thorbecke (FGT poverty measures were used to analyse the objectives, respectively. The result shows that number of years spent in school, household size and household assets were found to be the signifi cant determinants of the poverty status of the respondents. Household size had a negative eff ect on the household’s poverty status, while household assets and years of schooling had positive eff ects on status. The results of the FGT measures show that poverty incidence, depth and severity are higher in the low-income class than in the high-income class. In conclusion, farmers who are members of larger households, those with little or no formal education and those with minimal assets were poorer than others. It is therefore recommended that a family-planning campaign and farmers’ educational programs should be intensifi ed in the study area.

  19. VEGF and eNOS Expression in Umbilical Cord from Pregnancy Complicated by Hypertensive Disorder with Different Severity

    Directory of Open Access Journals (Sweden)

    K. Bhavina

    2014-01-01

    Full Text Available Background. Reduced blood flow in hypertensive pregnancy may influence the production vasoconstrictors; subsequently the vessel remains in highly contracted state. NO is a vasodilator; VEGF influences its synthesis by regulating eNOS production. Aim of our study was to evaluate the expression of VEGF and eNOS in different severity of hypertensive pregnancy. Methods. Study was conducted in 4 groups with 40 members: group 1—control, group 2—gestational hypertension, group 3—mild preeclampsia, and group 4—severe preeclampsia. Fetal end of umbilical cord was taken and follows IHC staining protocol for VEGF and eNOS antibody. Staining intensity were measured by semiquantitative scoring method. Mann Whitney U test was used to compare each group. Results. Decreased expression of both VEGF and eNOS was found in hypertensive condition than in normal condition. Among hypertensive group, severe preeclamptic group showed more intensity in staining than gestational hypertension and mild preeclampsia. Conclusion. Reduction of VEGF and eNOS in gestational hypertension may lead to hypoperfusion and subsequent hypoxia of fetus in hypertensive pregnancy. The developed hypoxic state may upregulate the synthesis of VEGF and thereby eNOS. Increased expression of VEGF and eNOS in severe group may be a compensatory mechanism to dilate the blood vessels and to improve blood flow of fetus.

  20. Ultimate strength analysis of thin plated structures using eigen-functions. 3rd Report. Application to reliability analysis; Koyu kansu wo mochiita usuita kozobutsu no dansosei kaisekiho. 3. Shinraisei kaiseki eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ueda, Y. [Osaka University, Osaka (Japan). Welding Research Institute; Masaoka, K.; Okada, H. [University of Osaka Prefecture, Osaka (Japan). Faculty of Engineering

    1996-12-31

    A reliability analysis was performed on ultimate strength of a hull by introducing reliability engineerings into the idealized structural unit method. Elements developed under the present study were applied to a model of an actual structure to indicate that even an analysis requiring much time under the finite element method can be performed in a short time and at high accuracy when this method is used. Analysis acted with bending moment and shear force simultaneously was performed on a model used as a structure in experiments carried out by Nishihara, assuming pure bending moment and longitudinal strength during slamming. Then, a reliability analysis was conducted on the same model based on this analysis method to investigate the ultimate strength. In an analysis of an ultimate strength when bending and shearing that assume slamming act upon simultaneously, axial force in the hull side decreases as loading increases, wherein how the shearing force increases can be identified clearly. Although existence of initial bends reduces the strength, the effect of variance in the vicinity of the average value on the reliability is rather small, while the effect due to variance in yield stress is greater. 27 refs., 14 figs., 4 tabs.

  1. Equivalent CTOD concept based on the local approach and its application to fracture performance evaluation of welded joints; Local approach ni motozuku toka CTOD gainen no teian to tsugite hakai seino hyoka eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ohata, M.; Minami, F.; Toyoda, M. [Osaka University, Osaka (Japan). Faculty of Engineering; Tanaka, T.; Arimochi, K. [Sumitomo Metal Industries, Ltd., Osaka (Japan); Glover, A. [Nova Gas Transmission Ltd., Calgary (Canada); North, T. [University of Toronto (Canada)

    1996-12-31

    A proposal was given on an equivalent crack tip opening displacement (CTOD) concept which relates quantitatively fracture performance of a structural member with the result of a three-point bending CTOD test via the Weibull stress based on a local approach. The equivalent CTOD is defined as a CTOD in which a three-point CTOD test piece and a structural member provide the same Weibull stress. Experimental and analytical discussions were performed on X80 steel welded joints. Effectiveness of the equivalent CTOD concept was verified from the fact that the fracture performance in welded joints with a large width estimated from the result of the three-point bending CTOD test using the equivalent CTOD concept showed good correspondence with the fracture performance obtained in the experiments. On the other hand, the result of estimation using the conventional CTOD concept is considerably smaller than measurements. As an application of the equivalent CTOD concept, a new determination procedure was introduced on required fracture tenacity to ensure deformation performance required on structural elements. The required CTOD value shows a trend that the smaller the ratio of yield stress of the welded metals to that in the base material, the greater the required CTOD grows. 16 refs., 19 figs., 3 tabs.

  2. Development of hydriding alloys with multi-functionally-graded properties and their applications to energy conversion devices; Keishagata fukugo kino wo hyomen ni motsu suiso kyuzo gokin no kaihatsu to energy henkan gijutsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Suda, S.; Kadoma, H.; Nagamoto, H.; Okura, T. [Kogakuin University, Tokyo (Japan)

    1997-02-01

    This paper describes the formation of fluoride layer on the surface of hydriding alloys. The fluoride formation reaction consists of a reduction removal process of surface oxide and a fluorination process. Specific surface area of alloy grains can be increased by the hydrogenation reaction in the surface layer accompanied with the removal of surface oxide, which results in easier permeation of molecular hydrogen into fluoride layer. During the fluorination process, a large amount of Ni in the alloy components is eluted, which results in the reduction of Ni distribution immediately under the fluoride layer in the alloy. Consequently, collector sites near the surface are reduced, and conductivity among alloy grains is degraded. To enhance the hydrogen collector sites, specific surface area of alloy grains can be increased by controlling the pH value of fluorination treatment solution in a given range. Moreover, performance of fluoride layer can be advanced by electrochemically dispersing metal Ni in the fluoride layer using Ni complex ion mixed in the treatment solution. 2 refs., 3 figs.

  3. FY1995 preparation of magnetic films with nano size magnetic domain structure and application for magnetic recording media with ultra-high density; 1995 nendo nano jiku seigyo jisei usumaku no sosei to chokomitsudo jiki kiroku media eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    It is required to control microstructure and micro magnetic behavior in Co-Cr based alloy thin films strictly in order to apply these thin films to the ultra high density magnetic recording media. In this study, thin film deposition technology to control nano-metric micro-structure and micro magnetic properties, especially in the distribution through thickness direction, were examined. Furthermore, to develop some evaluation technique to determine such local distribution of magnetic properties was one of the purpose of this study. The relationship between c-axis orientation of crystallites and distribution of the perpendicular coercivity H{sub c} in Co-Cr films has been investigated through the evaluation of the the H{sub c} of surface layer H{sub c}(s) measured by Kerr hysteresis loop tracer. Better c-axis orientation, higher Cr content and addition of Ta seemed to be essential requirements to get higher H{sub c} of initial growth layer H{sub c}(i) and good uniformity of H{sub c}. Lower {delta}H{sub c} caused better recording characteristics and lower media noise level. It has been clarified that the homogeneity of the magnetic properties in the film and c-axis orientation in the initial layer of the films were the essential factors to deposit low noise and high performance recording media. (NEDO)

  4. FY 1998 report on the results of R and D projects by local consortiums for immediate effects. Development of woody ceramics and application to building parts; 1998 nendo woody ceramics no sosei to kenchiku buzai nado eno oyo seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    The R and D project has been implemented for developing materials for, e.g., light, highly heat-insulating and energy-saving type building parts (e.g., roofing tiles and tiles), and light tableware having warmness of wood. The stock materials for china and porcelain, roofing tile clay, tiles and cement are dispersed with hollow microcapsules as the filler, which are of glassy balloons thermally treated to be highly functional, in order to develop superlight, highly heat insulating and high-strength materials. For development of the woody ceramic materials, the microcapsules are investigated for size, thickness, thermal shrinkage, fusibility, compressive stress, reinforcing mechanisms, and monodispersibility. Also investigated are optimization of material formability, development of commercial ceramics and evaluation of their marketability, decoration techniques, antimicrobial membrane materials and their immobilization techniques, development of antimicrobial ceramic tiles, evaluation of the antimicrobial membranes, evaluation of resistance to microwaves, and so on. The efforts are also directed to researches on fundamental techniques for woody tile clay, and R and D of the application techniques and products of the woody cement. (NEDO)

  5. Research of the elastic waves generated by a pulse laser. Excitation mechanism of elastic waves and application to nondestructive testing; Pulse laser de reikishita danseiha ni kansuru kenkyu. Danseiha reiki no mechanism to hihakai kensa eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Cho, H.; Takemoto, M. [Aoyama Gakuin University, Tokyo (Japan). College of Science and Engineering

    1994-07-20

    A bulk wave is generated when a pulse laser is irradiated to the material, and the characteristics of a Young`s modulus and Poisson`s ratio can be nondestructively estimated from the bulk wave. The generation mechanism of laser ultrasonic waves must be first clarified for such application. In this paper, fundamental research was conducted to study the generation mechanism of the elastic waves excited by a Q-switched Nd-YAG laser, and the generation method and characteristics of Rayleigh waves. The following result was obtained. A bulk wave is generated by the disk-like adiabatic expansion near the surface if the laser power is small when a spot-shape pulse laser was irradiated. A bulk wave is generated by the thin disk-like adiabatic expansion beneath the surface due to the thermal diffusion in the depth direction of a base material when the laser power becomes large. Moreover, a bulk wave is generated by the impact force due to abrasion and plasma when the power becomes still larger. The information on the bulk wave characteristics and Rayleigh wave was also obtained. 25 refs., 15 figs., 1 tab.

  6. Investigation of several dynamic processes on solid surfaces and its application to the extreme high vacuum technology. Kotai hyomenjo no doteki katei no kaimei to sono goku koshinku gijutsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Fujita, D.; Honma, T. (The Univ. of Tokyo, Tokyo (Japan). Inst. of Industrial Science)

    1991-08-26

    This paper makes fundamental studies on several dynamic processes on solid surfaces such as adsorption, desorption, surface segregation, surface precipitation and initial oxidation. The studies aim at applying the information and knowledge obtained to development of an extreme high vacuum technology. Theoretical and technological consideration is given to quantification and precision of surface analytical methods necessary and indispensable for a base technology for the study. The paper pays particular attention to the following three surface analytical methods: Auger electron spectroscopy for a composition analysis, surface extended electron energy loss fine strucutre as a surface short-distance order structural analysis, and thermal sesorption spectroscopy as an adsorption/desorption process analysis. These methods are applied to fundamental studies for development of materials required for creation of an extreme high vacuum. Taking an outgassing phenomenon in the extreme high vacuum as a set of various dynamic processes, quantitative measurement and a mechanism analysis of outgassing are conducted. Actually, the extreme high vacuum is trially produced. 244 refs., 128 figs., 21 tabs.

  7. Estimating method for a phase-velocity of Rayleigh waves on multi-layered media and its application to inversion problem; Rayleigh ha no bunsan kyokusen no kinji keisanho no teian to chika kozo suitei eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Konno, K. [Shibaura Institute of Technology, Tokyo (Japan)

    1997-10-15

    An attempt was made to approximate a dispersion curve being a function of S-wave velocity, P-wave velocity, density and layer thickness by using only the S-wave velocity and the layer thickness. Then, this approximation method was utilized for an attempt of deriving the S-wave velocity structure directly from the observed dispersion curve. Lastly, the derived S-wave velocity structure was attempted to be utilized as an initial model for inversion analysis. The pseudo-phase velocity derived by using this proposed method becomes functions for only the cycle, S-wave velocity and layer thickness, converges into the S-wave velocity of the lowermost layer at the extreme of a long cycle, and agrees with the S-wave velocity of the surface layer in the short cycle side. The pseudo-dispersion curve approximates the dispersion curve of Rayleigh waves more closely than that of Love waves. If depths and pseudo-wave lengths are in rough agreement in a region in which amplitude of the base mode Rayleigh waves is distributed largely, the pseudo-phase velocity resembles to the theoretical phase velocity. S-wave velocity at a ground depth which corresponds to a length of the Rayleigh wave could be estimated to some extent by differentiating the wavelength with regard to the cycle. 7 refs., 19 figs., 1 tab.

  8. Fiscal 1998 research report on application technology of molecular assemblies for a functional protein system (Development of advanced maintenance technology for power plants); 1998 nendo kinosei tanpakushitsu shugotai oyo gijutsu no kenkyu kaihatsu. Hatsuden shisetsuyo kokino mentenansu gijutsu kaihatsu seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Basic research was made on engineering reappearance and industrial use of the complex function of molecular assemblies for a functional protein system of organisms. In research on a light controllable reaction system by physicochemical technique, construction of a light controllable bioreactor is in promotion. In research on a light controllable reaction system by biochemical technique, researches are in promotion on structure and correlation analysis of photosynthetic protein complex, stabilization of an activity, and conversion of light energy to membrane transport energy using cyanobacteria as material. In research on a molecular recognition controllable reaction system in a fine membrane region, correlation analysis of the structure and function of glutamine receptor channels is in promotion. In addition, researches were made on reconstitution of endocytic micromachinery, a multi-enzyme system for synthesis of glycosphingolipids, a complex redox system by genetic engineering technique, and application technology of molecular assemblies for a functional protein system. (NEDO)

  9. Report on achievements in fiscal 1998. Venture business growing type consortium - small business creating infrastructure (Development of ultra-high efficiency cultivation system for Hyphomycetes, and application of the system to plant device to manufacture fermentative foods); 1998 nendo itojokin no chokoritsu tairyo baiyo system no kaihatsu to kinosei hakko sho0kuhin seizo plant kiki eno tekiyo seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    This project has noticed the membrane surface liquid culture method, and researched and developed the following themes with an objective to commercialize the membrane surface liquid culture system and develop novel fermentative foods using this system. Cultivation of Beni-koji bacteria (solid culture of M. pilosus k) is taken up in the model cultivation system. (1) establishment of the membrane surface liquid culture system and development of devices, (2) elucidation of cultivation characteristics of the membrane surface liquid culture system and optimization of the cultivating conditions, and (3) development of fermentative foods. In Item (1), such problems were solved as automation of bacteria planting, long-term pasteurized cultivation, and assurance of clean cultivation broth. In Item (2), it was discovered most suitable that maltose concentration is about 5%, glutamic acid concentration is about 50 mM, the initial culture medium pH is about six, and the cultivation temperature is 30 degree C. In item (3), as a result of mixing this red coloring matter containing cultivation broth into preparation of Miso (bean paste) and soy sauce, products having high {gamma}-Aminobutyric acid and monacholin k content were developed. In addition, while Beni-koji made by solid cultivation had peculiar taste and odor, this drawback was eliminated by using this coloring matter containing cultivation broth. This has made differentiation from conventional products possible. (NEDO)

  10. National Health Insurance Scheme: How Protected Are Households in Oyo State, Nigeria from Catastrophic Health Expenditure?

    Directory of Open Access Journals (Sweden)

    Olayinka Stephen Ilesanmi

    2014-05-01

    Full Text Available Background The major objective of the National Health Insurance Scheme (NHIS in Nigeria is to protect families from the financial hardship of large medical bills. Catastrophic Health Expenditure (CHE is rampart in Nigeria despite the take-off of the NHIS. This study aimed to determine if households enrolled in the NHIS were protected from having CHE. Methods The study took place among 714 households in urban communities of Oyo State. CHE was measured using a threshold of 40% of monthly non-food expenditure. Descriptive statistics were done, Principal Component Analysis was used to divide households into wealth quintiles. Chi-square test and binary logistic regression were done. Results The mean age of household respondent was 33.5 years. The median household income was 43,500 naira (290 US dollars and the range was 7,000–680,000 naira (46.7–4,533 US dollars in 2012. The overall median household healthcare cost was 890 naira (5.9 US dollars and the range was 10-17,700 naira (0.1–118 US dollars in 2012. In all, 67 (9.4% households were enrolled in NHIS scheme. Healthcare services was utilized by 637 (82.9% and CHE occurred in 42 (6.6% households. CHE occurred in 14 (10.9% of the households in the lowest quintile compared to 3 (2.5% in the highest wealth quintile (P= 0.004. The odds of CHE among households in lowest wealth quintile is about 5 times. They had Crude OR (CI: 4.7 (1.3–16.8, P= 0.022. Non enrolled households were two times likely to have CHE, though not significant Conclusion Households in the lowest wealth quintiles were at higher risk of CHE. Universal coverage of health insurance in Nigeria should be fast-tracked to give the expected financial risk protection and decreased incidence of CHE.

  11. Cataract blindness and barriers to cataract surgical intervention in three rural communities of Oyo State, Nigeria.

    Science.gov (United States)

    Oluleye, T S

    2004-01-01

    The study was a community based field survey that used a rapid assessment method to determine the prevalence of cataract blindness in people aged 50 years and above in 3 rural communities. The purpose of the study was to determine the prevalence of cataract blindness and barriers to cataract surgical intervention in an area served by a health facility managed by a tertiary institution. Abedo, Akinyele and Ketepe Villages in Akinyele Local Government Area of Oyo State Nigeria were selected based on their nearness to the primary health care centre in Abedo. A total of 477 persons aged 50 years and above were seen being 73.3 percent of expected. Those with visual acuity of less than 3/60 in an eye and those with visual acuity of less than 3/60 in the better eye were examined in more details using a pen torch, an ophthalmoscope and tonometer to determine the cause of blindness. All persons who have had surgery were examined. The prevalence of blindness in persons aged 50 years and above was 1.47% and that of cataract blindness in the same age group in the villages was 0.84% constituting 57.14% of blindness. The main barriers to hospital presentation were cost of surgery (52.8%) and distance to hospital (33.8%). The constraints and limitations encountered during the study included rural-urban migration and population discrepancies between what obtained at the villages and those supplied by the Local Government Population Commission. It is hoped that this study will serve as a preliminary survey and a base line for further studies and the initiation of a blindness prevention programme in the area.

  12. An artificial compression method for ENO schemes: The slope modification method

    Science.gov (United States)

    Yang, Huanan

    1988-01-01

    A simple and effective method of artificial compression is introduced. This method is based on a modification of the slopes of the ENO (essentially nonoscillatory) reconstruction and, with the help of suitable chosen parameters, greatly improves the resolution of the contact discontinuities. Numerical examples are provided to test the performance of the method and to give some suggestions as to the choice of the parameters.

  13. Role of eNOS in water exchange index maintenance-MRI studies

    Science.gov (United States)

    Atochin, D.; Litvak, M.; Huang, S.; Kim, Y. R.; Huang, P.

    2017-08-01

    Stroke studies employ experimental models of cerebral ischemic and reperfusion injury in rodents. MRI provides valuable supravital data of cerebral blood flow and brain tissue damage. This paper presents MRI applications for cerebral blood flow research in mice lines with impaired nitric oxide production by endothelial nitric oxide synthase. Our data demonstrates that specific modifications of MRI methodology in transgenic mouse models help to evaluate the role of eNOS in the brain-blood barrier function.

  14. Determinants of Food Security Status of Maize-Based Farming Households in Southern Guinea Savannah Area of Oyo State, Nigeria.

    Directory of Open Access Journals (Sweden)

    Oluwayemisi Abidemi Onasanya

    2016-06-01

    Full Text Available Nigeria is one of the countries in sub-Saharan Africa with insufficient food and high food import bill, which have debilitating effects on the productive capacity of the citizens. Maize is the most important cereal after rice and its production contributes immensely to food availability on the tables of many Nigerians. This study examined the contribution of maize production to household food security status of rural maize-farming households in the southern guinea savannah of Oyo state, Nigeria. A multistage sampling procedure was used to select 200 farm households and the data were analysed using descriptive statistics, recommended daily calorie requirement (RDCR approach, Logit model. Results showed that about three-quarters of the households were food secure and were able to meet the recommended calorie intake of 2260Kcal per capita per day. The shortfall index (P which measures the extent of deviation from the food security line, indicated that the food secure households exceeded the RDCR by 65%, while the food insecure households fell short of the RDCR by 31%. The logit model showed that maize output, gender, primary occupation of the farmer, farm size and farming experience had a positive influence on food security status while age had a negative influence on the food security status of maize-based farming households in the Southern Guinea Savannah of Oyo State, Nigeria. This suggests need for specific support to improve maize production

  15. A survey of rabies virus antibodies in confined, hunting and roaming dogs in Ogun and Oyo States, Southwestern Nigeria

    Directory of Open Access Journals (Sweden)

    Daniel Oladimeji Oluwayelu

    2015-01-01

    Full Text Available Objective: To screen for rabies virus (RABV antibodies in apparently healthy confined, hunting and roaming dogs by a community-based approach. Methods: Sera from 230 (80 confined, 92 hunting and 58 roaming dogs in some urban and periurban communities in Ogun and Oyo states, Southwestern Nigeria were screened for RABV antibodies using the indirect ELISA method. Results: Analysis of administered questionnaires showed that of 80 confined dog owners, 37 were aware of anti-rabies vaccination (i.e. they were informed while 17 were negligent and 26 uninformed. Of the 230 sera tested, only 13 (5.7% from vaccinated confined dogs in Oyo state were positive (i.e. had optimal RABV antibody titres (mean 0.54, 95% CI: 0.42–0.67 while all confined dog sera in Ogun state were negative. Eleven (12.0% and 14 (24.1% of the hunting and roaming dogs respectively had sub-optimal RABV antibody titres while the rest were negative. Conclusions: Evidently, these groups of dogs are a totally unprotected and susceptible dog population that can serve as potential reservoirs of RABV in the study area. Responsible pet ownership, vaccination of hunting and roaming dogs, and community-based active rabies surveillance are therefore advocated in Nigeria.

  16. Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

    Energy Technology Data Exchange (ETDEWEB)

    Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri; Yamamori, Tohru [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan); Niwa, Koichi [Laboratory of Biochemistry, Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493 (Japan); Hattori, Yuichi [Department of Molecular and Medical Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Kondo, Takashi [Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194 (Japan); Inanami, Osamu, E-mail: inanami@vetmed.hokudai.ac.jp [Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818 (Japan)

    2015-01-02

    Highlights: • eNOS activity is increased in BAECs exposed to X-rays. • ATM is involved in this increased eNOS activity. • HSP90 modulates the radiation-induced activation of ATM and eNOS. - Abstract: In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.

  17. Achievement report on developing superconductor power applied technologies in fiscal 1999 (1). Research and development of superconductor wire materials, research and development of superconductor power generators, research of total systems, research and development of freezing systems, and verification tests; 1999 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 1. Chodendo senzai no kenkyu kaihatsu / chodendo hatsudenki no kenkyu kaihatsu / total system no kenkyu / reito system no kenkyu kaihatsu / jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    With an objective to achieve higher efficiency, higher density, and higher stability in power systems, research and development has been performed on superconductor power applied technologies. This paper summarizes the achievements thereof in fiscal 1999. In research and development of the superconductor wire materials, decrease in loss and increase in capacity of the conductors were progressed for the Nb{sub 3}Sn wire material, whereas its mechanical properties and stability were evaluated. In research and development of the superconductor generators, an ultra high speed responding generator was verified of its healthiness in a sudden short circuit test. A linkage test with an operating 77-kV system was performed, wherein verification was given that the superconductor generator can be operated stably against various disturbances. In research and development of the freezing systems, an improved system was structured, which achieved operation of 11,390 hours in a single system as a result of the high reliability of the oil-free structure. In the verification tests, the ultra high speed responding model generator was connect to the freezing system to give such tests as load test, onerous test, actuation test by using the M-G system, and 77-kV system linkage test. The functions, reliability, and durability of the system were verified, and different data were acquired. (NEDO)

  18. A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

    Directory of Open Access Journals (Sweden)

    Loo Keat

    2012-05-01

    Full Text Available Abstract Background Hyperhomocysteinemia as a consequence of the MTHFR 677 C > T variant is associated with cardiovascular disease and stroke. Another factor that can potentially contribute to these disorders is a depleted nitric oxide level, which can be due to the presence of eNOS +894 G > T and eNOS −786 T > C variants that make an individual more susceptible to endothelial dysfunction. A number of genotyping methods have been developed to investigate these variants. However, simultaneous detection methods using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP analysis are still lacking. In this study, a novel multiplex PCR-RFLP method for the simultaneous detection of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C variants was developed. A total of 114 healthy Malay subjects were recruited. The MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C variants were genotyped using the novel multiplex PCR-RFLP and confirmed by DNA sequencing as well as snpBLAST. Allele frequencies of MTHFR 677 C > T and eNOS +894 G > T and eNOS −786 T > C were calculated using the Hardy Weinberg equation. Methods The 114 healthy volunteers were recruited for this study, and their DNA was extracted. Primer pair was designed using Primer 3 Software version 0.4.0 and validated against the BLAST database. The primer specificity, functionality and annealing temperature were tested using uniplex PCR methods that were later combined into a single multiplex PCR. Restriction Fragment Length Polymorphism (RFLP was performed in three separate tubes followed by agarose gel electrophoresis. PCR product residual was purified and sent for DNA sequencing. Results The allele frequencies for MTHFR 677 C > T were 0.89 (C allele and 0.11 (T allele; for eNOS +894 G > T, the allele frequencies were 0.58 (G allele and 0.43 (T allele; and for eNOS −786 T > C, the allele

  19. Social support seeking and self-efficacy-building strategies in enhancing the emotional well-being of informal HIV/AIDS caregivers in Ibadan, Oyo state, Nigeria.

    Science.gov (United States)

    Okeke, Bernedette Okwuchukwu

    2016-01-01

    This study examined the relative efficacy of social support seeking (SSS) and self-efficacy building (SEB) in the management of emotional well-being of caregivers of people suffering from HIV/AIDS. It was based at the United States President's Emergency Plan for AIDS Relief (PEPFAR) center in the University College Hospital, Ibadan, Oyo state, being the first and the largest teaching hospital in Nigeria. A 3 × 2 factorial design consisting of treatment and a control group was used. The columns have two levels of gender being male and female caregivers. One-hundred and sixty-five (165) caregivers who were taking care of people that are suffering from HIV/AIDS were purposively selected and randomly assigned to the treatment groups and control. The treatment was carried out for a period of eight weeks. Two null hypotheses were tested, both at .05 levels of significance. Data were collected with the use of standardized intruments rating scale; social support scale, general self-efficacy scale and emotional well-being scale. ANCOVA was used to establish significant treatment effects with the pretest as covariate. Even though SSS and SEB were both found to be effective in enhancing the emotional well-being of informal caregivers in this study when compared to the controls, SSS was significantly more effective than SEB in achieving this goal. Since the HIV/AIDS patients cannot be adequately cared for in the hospital settings due to severe shortages of material, personnel and time, serious efforts should be made by the three levels of the health care system viz: the primary, secondary and tertiary health care systems, to encourage the employment of the psychological management of caregivers of people suffering from HIV/AIDS. Also, the psychologists, clinical psychologists and the significant others should be encouraged to employ this psychological management in the care of HIV/AIDS informal caregivers.

  20. Aspirin prevents TNF-α-induced endothelial cell dysfunction by regulating the NF-κB-dependent miR-155/eNOS pathway: Role of a miR-155/eNOS axis in preeclampsia.

    Science.gov (United States)

    Kim, Joohwan; Lee, Kyu-Sun; Kim, Ji-Hee; Lee, Dong-Keon; Park, Minsik; Choi, Seunghwan; Park, Wonjin; Kim, Suji; Choi, Yoon Kyung; Hwang, Jong Yun; Choe, Jongseon; Won, Moo-Ho; Jeoung, Dooil; Lee, Hansoo; Ryoo, Sungwoo; Ha, Kwon-Soo; Kwon, Young-Guen; Kim, Young-Myeong

    2017-03-01

    Preeclampsia is an inflammatory disease with endothelial cell dysfunction that occurs via decreased endothelial nitric oxide synthase/nitric oxide (eNOS/NO) activity. Aspirin reduces the incidence of hypertensive pregnancy complications. However, the underlying mechanism has not been clearly explained. Here, we found that tumor necrosis factor (TNF)-α, microRNA (miR)-155, and eNOS levels as well as endothelial redox phenotype were differentially regulated in preeclamptic patients, implying the involvement of TNF-α- and redox signal-mediated miR-155 biogenesis and eNOS downregulation in the pathogenesis of preeclampsia. Aspirin prevented the TNF-α-mediated increase in miR-155 biogenesis and decreases in eNOS expression and NO/cGMP production in cultured human umbilical vein endothelial cells (HUVECs). Similar effects of aspirin were also observed in HUVECs treated with H2O2. The preventive effects of aspirin was associated with the inhibition of nuclear factor-κB (NF-κB)-dependent MIR155HG (miR-155 host gene) expression. Aspirin recovered the TNF-α-mediated decrease in wild-type, but not mutant, eNOS 3'-untranslated region reporter activity, whose effect was blocked by miR-155 mimic. Moreover, aspirin prevented TNF-α-mediated endothelial cell dysfunction associated with impaired vasorelaxation, angiogenesis, and trophoblast invasion, and the preventive effects were blocked by miR-155 mimic or an eNOS inhibitor. Aspirin rescued TNF-α-mediated eNOS downregulation coupled with endothelial dysfunction by inhibiting NF-κB-dependent transcriptional miR-155 biogenesis. Thus, the redox-sensitive NF-κB/miR-155/eNOS axis may be crucial in the pathogenesis of vascular disorders including preeclampsia. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Fiscal 2000 achievement report on the research and development of medical and welfare apparatus/technology. Totally implantable artificial heart for clinical use; 2000 nendo iryo fukushi kiki gijutsu kenkyu kaihatsu seika hokokusho. Rinsho oyo ni muketa tainai umekomigata jinko shinzo system

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-05-01

    In the development of control technologies for a pulsatile pump, studies proceeded with a view to using a mixed venous blood oxygen saturation sensor, an ultrasonic sensor, and an absolute pressure sensor. Efforts were started to downsize the hydraulic actuator and to enhance its efficiency. In the development of a non-pulsatile artificial heart, the GyroP1710 series of artificial heart systems of Japanese make was developed, which employed a curved inlet known to be highly compatible from the anatomical point of view. In the development of a total system, a pulsatile auxiliary artificial heart was given a chronic animal test which continued approximately three weeks, when it yielded a flow of approximately 4 liters/minute showing that it would be practical as an auxiliary heart. As for the non-pulsatile artificial heart, a cardiopulmonary bypass test was conducted, when the blood circulation behavior was normal and organ functions were sustained. The free hemoglobin level was 9.3mg/dl achieving the target value, and the test animal lived as long as 50 days. (NEDO)

  2. Assessment of Mobile Health Nursing Intervention Knowledge among Community Health Nurses in Oyo State, Nigeria.

    Science.gov (United States)

    Titilayo, Odetola D; Okanlawon, F A

    2014-09-01

    Maternal mortality is high in Nigeria especially in rural areas due to knowledge deficit about expected care and labour process, socio-cultural belief, health care workers' attitude, physical and financial barriers to quality health care access. Mobile health (m-health) technology which is the use of mobile telecommunication devices in health care delivery reduces costs, improves care access, removes time and distance barriers and facilitates patient-provider communications needed to make appropriate health decisions. Previous studies empowering nurses with m-health knowledge resulted in improved uptake of health care services. There exists a literature dearth about knowledge and perception of nurses in Nigeria. This study became expedient to empower nurses working at the grassroots with the knowledge of m-health and assess the impact of educational training on their perception of its effectiveness. This quasi-experimental study carried out in four randomly selected LGAs across Oyo South Senatorial district involved participants at experimental (20 nurses) and control levels (27 nurses). A validated 25-item questionnaire explored nurses' perception, knowledge and perceived effectiveness of m-health in improving uptake of maternal health services in Nigeria among both groups before intervention. Intervention group nurses had a training equipping them with knowledge of m-health nursing intervention (MNHI) for a period of one week. Their perception, knowledge and perceived effectiveness were re-assessed at three-months and six-months after MHNI. Data were analyzed using Chi-square and repeated measures ANOVA at 5% significance level. In the EG, knowledge score significantly increased from 21.9±4.5 at baseline to 23.6±4.6 and 23.2±5.6 at three-month and six-month respectively while there was no significant difference in knowledge score among CG over the study period. A very significant difference was shown in the knowledge and perception of mobile health and its

  3. Contraceptive prevalence and determinants among women of reproductive age group in Ogbomoso, Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Adeyemi AS

    2016-03-01

    Full Text Available Adewale S Adeyemi,1 Adenike I Olugbenga-Bello,2 Oluwatosin A Adeoye,3 Moshood O Salawu,3 Adesola A Aderinoye,3 Michael A Agbaje1 1Department of Obstetrics and Gynaecology, 2Department of Community Medicine, Faculty of Clinical Sciences, College of Health Sciences, Ladoke Akintola University of Technology (LAUTECH, Osogbo, Osun State, 3Department of Community Medicine, LAUTECH Teaching Hospital, Ogbomoso, Oyo State, Nigeria Background: The fertility rate in Nigeria is 5.7 children per woman. The contraceptive prevalence rate has been found to be low at 15% in 2013, compared to other countries such as the US and Pakistan. Objective: The study aimed to assess the contraceptive prevalence among women of reproductive age in Ogbomoso town, and determinants of use, with a view to make appropriate recommendations that will enhance the uptake of family planning services. Materials and methods: This is a descriptive cross-sectional study conducted with 560 respondents, using a multistage sampling technique. Data were retrieved using a semi-structured, pretested questionnaire. Results: All the respondents were aware of contraception; however, only 49.7% (271 had ever used any method, while 25.4% (69 of the number who had ever used contraception were currently using a method. The methods being used were the traditional type (four [5.9%], natural type (two [3.0%], and modern type (63 [91.1%]. The predictors of contraception use included the age group of 40–49 years (odds ratio [OR] 14.1; confidence interval [CI] 3.06–73.24; P=0.0001; the married women were approximately four times more likely to use contraception than the single women (OR 4.5; CI 3.03–6.72; P<0.0001. The women with tertiary level of education were three times more likely to use contraception than those without formal education (OR 3.1; CI 1.13–9.95; P=0.0268, and the odds ratio of respondents with a positive attitude to using contraception more than those with negative attitude was 2

  4. Report on results for fiscal 1997 on development of superconducting electric power application technology. Pt. 1. R and D of superconducting wire, R and D of superconducting generator, studies on total system, R and D of refrigeration system, and verification test; 1997 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 1. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total system no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    This report explains the outline as Part 1. In fiscal 1997, the 10th year of the project, a multi cylindrical rotary model for which an in-situ verification test was finished was brought back to the plant and dismantled for examination, while the in-situ verification test of a slow-response type model machine rotor was conducted in combination with a refrigeration system. In addition, in the research of AC wire materials and oxide based materials, studies were made with a purpose of high characterization and long wire materialization. In the metallic materials, a 10kANbTi conductor was developed while, in oxide-based materials, research was done on performance improvement and wire materialization based on various synthesizing methods. The manufacturing, factory test and in-situ text were conducted for a 70,000kW model machine with the purpose of R and D of a 200,000kW class pilot machine. Examination was made on the test method of the 70,000kW class model machine, operation technology of a superconducting generation system, and the effect of introducing the superconducting generator into a power system. In the conventional refrigeration system, a single unit test was carried out for the liquefaction, liquid storing capacity, etc., of the system. The 70,000kW class model machine was put through a test for confirming the general operation including the refrigeration system. (NEDO)

  5. KONSUMSI MIKRONUTRIEN DAN EKSPRESI GEN eNOS3 ALEL INTRO 4a4b PADA PENDERITA HIPERTENSI ETNIK MINANGKABAU

    Directory of Open Access Journals (Sweden)

    Delmi Sulastri

    2011-05-01

    Full Text Available AbstrakGen eNOS3 varian intron 4a4b merupakan salah satu gen yang berhubungan dengan kejadian hipertensi esensial. Kemampuan Gen eNOS3 varian ini untuk mensintesis enzim nitric oxide synthase (NOS berkurang sehingga sintesis Nitric oxide (NO menurun. Senyawa NO adalah suatu senyawa vasoaktif yang menyebabkan vasodilatasi pembuluh darah.Penelitian pada subjek hipertensi dan normotensi berusia 30-65 tahun bertujuan untuk melihat pengaruh mikronutrien terhadap ekspresi gen eNOS3 alel intron 4a4b. 130 subjek, diwawancara pola makannya lalu dilakukan analisis asupan mikronutrien, gen eNOS3, serta kadar NO plasma. Data yang diperoleh dianalisis menggunakan uji T dan chi-square.Kadar NO plasma penderita hipertensi adalah 26.91 + 15.40 μM/L dan normotensi 25.79 + 15.04 μM/L. 55.5% subjek hipertensi memiliki gen eNOS3 varian intron 4b dan 69% subjek normotensi mempunyai varian intron 4a4b. Kadar NO plasma rendah, (66.7% ditemukan pada subjek hipertensi dengan varian intron 4b. Tidak terdapat hubungan bermakna asupan mikronutrien dengan kadar NO plasma pada subjek hipertensi.Hasil ini tidak mendukung pengaruh konsumsi mikronutrien terhadap kadar NO plasma pada penderita hipertensi dengan gen eNOS3 varian intron 4a4b.Kata Kunci : hipertensi esensial, gen eNOS3, kadar NO plasmaAbstracteNOS 3 gene intron 4a4b allel is one of the important genes which is related to hypertesion essensial. This gene expresses the nitric oxide synthase (NOS enzyme which regulates the synthesis of Nitric oxide (NO in human body. NO causes vasodilatation, which decreases peripheral resistant and blood pressure.A research has been done based on cross sectional study on hypertension patients and those with normal blood pressure in the range of 30-65 years old. The main of this research is to evaluate the influence of micronutrient consumption on eNOS3 gene intron 4a4b allel expression in hypertension subjects in Minangkabau ethnicity. 130 subjects interviewed about micronutrient

  6. Early ischaemic preconditioning requires Akt- and PKA-mediated activation of eNOS via serine1176 phosphorylation.

    Science.gov (United States)

    Yang, Changjun; Talukder, M A Hassan; Varadharaj, Saradhadevi; Velayutham, Murugesan; Zweier, Jay L

    2013-01-01

    The role of endothelial nitric oxide synthase (eNOS)/NO signalling is well documented in late ischaemic preconditioning (IPC); however, the role of eNOS and its activation in early IPC remains controversial. This study investigates the role of eNOS in early IPC and the signalling pathways and molecular interactions that regulate eNOS activation during early IPC. Rat hearts were subjected to 30-min global ischaemia and reperfusion (I/R) with or without IPC (three cycles 5-min I and 5-min R) in the presence or absence of the NOS inhibitor l-NAME, phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 (LY), and protein kinase A (PKA) inhibitor H89 during IPC induction or prior endothelial permeablization. IPC improved post-ischaemic contractile function and reduced infarction compared with I/R with this being abrogated by l-NAME or endothelial permeablization. eNOS(Ser1176), Akt(Ser473), and PKA(Thr197) phosphorylation was increased following IPC. I/R decreased eNOS(Ser1176) phosphorylation, whereas IPC increased it. Mass spectroscopy confirmed eNOS(Ser1176) phosphorylation and quantitative Western blots showed ∼24% modification of eNOS(Ser1176) following IPC. Immunoprecipitation demonstrated eNOS, Akt, and PKA complexation. Immunohistology showed IPC-induced Akt and PKA phosphorylation in cardiomyocytes and endothelium. With eNOS activation, IPC increased NO production as measured by electron paramagnetic resonance spin trapping and fluorescence microscopy. LY or H89 not only decreased Akt(Ser473) or PKA(Thr197) phosphorylation, respectively, but also abolished IPC-induced preservation of eNOS and eNOS(Ser1176) phosphorylation as well as cardioprotection. Thus, Akt- and PKA-mediated eNOS activation, with phosphorylation near the C-terminus, is critical for early IPC-induced cardioprotection, with eNOS-derived NO from the endothelium serving a critical role.

  7. Challenge to unique researches; Unique na kenkyu eno challenge

    Energy Technology Data Exchange (ETDEWEB)

    Kato, M. [Central Research Inst. of Electric Power Industry, Tokyo (Japan)

    2000-05-10

    Mr. Takashi Tachibana, a critic, enumerates two reasons for why the U.S.A. is maintaining the high research level. One is the U.S. research institution in which foreigners can perform their actions freely. Another is the American frontier spirit that evaluates highly the challenge to new things. In the fields where the Central Research Institute of Electric Power Industry of Japan has achieved world-wide excellent accomplishments, many are the cases in which foreign researchers participate and work actively as the visiting researchers or the Science and Technology Agency (STA) fellows. In the latter case, the Central Research Institute of Electric Power Industry has introduced since a few years ago a system in which challenges can be made on unique researches that the researchers can draw their inspirations by themselves. Results are beginning to appear in several subjects, including the environmental hormone measuring method utilizing biotechnology. The 21st century requires solution of different problems in the electric power business, such as energy and environment issues. It would become increasingly important to work positively on creative and advanced researches and further strengthen the research power as the base for problem solution under the internationally opened research institution where first-class researchers inside and outside the country will get together. (NEDO)

  8. ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Xinghua; Miao, Xiaobing; Wu, Yaxun; Li, Chunsun; Guo, Yan; Liu, Yushan; Chen, Yali; Lu, Xiaoyun [Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu (China); Wang, Yuchan, E-mail: wangyuchannt@126.com [Department of Pathogen and Immunology, Medical College, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu (China); He, Song, E-mail: hesongnt@126.com [Department of Pathology, Affiliated Cancer Hospital of Nantong University, 30 North Tongyang Road, Pingchao, Nantong 226361, Jiangsu (China)

    2015-07-15

    Enolases are glycolytic enzymes responsible for the ATP-generated conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to the glycolytic function, Enolase 1 (ENO1) has been reported up-regulation in several tumor tissues. In this study, we investigated the expression and biologic function of ENO1 in Non-Hodgkin's Lymphomas (NHLs). Clinically, by western blot analysis we observed that ENO1 expression was apparently higher in diffuse large B-cell lymphoma than in the reactive lymphoid tissues. Subsequently, immunohistochemical staining of 144 NHLs suggested that the expression of ENO1 was significantly lower in the indolent lymphomas compared with the progressive lymphomas. Further, we identified ENO1 as an independent prognostic factor, and it was significantly correlated with overall survival of NHL patients. In addition, we found that ENO1 could promote cell proliferation, regulate cell cycle associated gene and PI3K/AKT signaling pathway in NHLs. Finally, we verified that ENO1 participated in the process of lymphoma cell adhesion mediated drug resistance (CAM-DR). Adhesion to FN or HS5 cells significantly protected OCI-Ly8 and Daudi cells from cytotoxicity compared with those cultured in suspension, and these effects were attenuated when transfected with ENO1-siRNA. Based on the study, we propose that inhibition of ENO1 expression may be a novel strategy for therapy for NHLs patients, and it may be a target for drug resistance. - Highlights: • ENO1 expression is reversely correlated with clinical outcomes of patients with NHLs. • ENO1 promotes the proliferation of NHL cells. • ENO1 regulates cell adhesion mediated drug resistance.

  9. Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism

    Science.gov (United States)

    Boo, Yong Chool; Hwang, Jinah; Sykes, Michelle; Michell, Belinda J.; Kemp, Bruce E.; Lum, Hazel; Jo, Hanjoong

    2002-01-01

    Shear stress stimulates nitric oxide (NO) production by phosphorylating endothelial NO synthase (eNOS) at Ser(1179) in a phosphoinositide-3-kinase (PI3K)- and protein kinase A (PKA)-dependent manner. The eNOS has additional potential phosphorylation sites, including Ser(116), Thr(497), and Ser(635). Here, we studied these potential phosphorylation sites in response to shear, vascular endothelial growth factor (VEGF), and 8-bromocAMP (8-BRcAMP) in bovine aortic endothelial cells (BAEC). All three stimuli induced phosphorylation of eNOS at Ser(635), which was consistently slower than that at Ser(1179). Thr(497) was rapidly dephosphorylated by 8-BRcAMP but not by shear and VEGF. None of the stimuli phosphorylated Ser(116). Whereas shear-stimulated Ser(635) phosphorylation was not affected by phosphoinositide-3-kinase inhibitors wortmannin and LY-294002, it was blocked by either treating the cells with a PKA inhibitor H89 or infecting them with a recombinant adenovirus-expressing PKA inhibitor. These results suggest that shear stress stimulates eNOS by two different mechanisms: 1) PKA- and PI3K-dependent and 2) PKA-dependent but PI3K-independent pathways. Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli.

  10. Exercise training enhanced myocardial endothelial nitric oxide synthase (eNOS function in diabetic Goto-Kakizaki (GK rats

    Directory of Open Access Journals (Sweden)

    Kaminski Pawel M

    2008-11-01

    Full Text Available Abstract Background Different mechanisms of diabetic-induced NO dysfunction have been proposed and central to most of them are significant changes in eNOS function as the rate-limiting step in NO bioavailability. eNOS exists in both monomeric and dimeric conformations, with the dimeric form catalyzing the synthesis of nitric oxide, while the monomeric form catalyzes the synthesis of superoxide (O2-. Diabetic-induced shifts to decrease the dimer:monomer ratio is thought to contribute to the degradation of nitric oxide (NO bioavailability. Exercise has long been useful in the management of diabetes. Although exercise-induced increases expression of eNOS has been reported, it is unclear if exercise may alter the functional coupling of eNOS. Methods To investigate this question, Goto-Kakizaki rats (a model of type II diabetes were randomly assigned to a 9-week running program (train or sedentary (sed groups. Results Exercise training significantly (p 4, but not in the presence of exogenous BH4. Exercise training also significantly decreased NADPH-dependent O2- activity. Conclusion Exercise-induced increased eNOS dimerization resulted in an increased coupling of the enzyme to facilitate production of NO at the expense of ROS generation. This shift that could serve to decrease diabetic-related oxidative stress, which should serve to lessen diabetic-related complications.

  11. Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome

    Directory of Open Access Journals (Sweden)

    Yuksel Seckin

    2016-01-01

    Full Text Available Background. There are no studies investigating the relationship between endothelial nitric oxide synthase (eNOS gene polymorphisms and hepatorenal syndrome (HRS. Aim. The purpose of this study is to elucidate whether eNOS gene polymorphisms (G894T and T-786C play a role in the development of type-2 HRS. Methods. This study was carried out in a group of 92 patients with cirrhosis (44 patients with type-2 HRS and 48 without HRS and 50 healthy controls. Polymorphisms were determined by polymerase chain reaction (PCR and melting curve analysis. Results. We did not find any significant difference in allele and genotype distributions of the eNOS -T-786C polymorphism among the groups (p=0.440. However, the frequency of GT (40.9% and TT (13.6% genotypes and mutant allele T (34.1% for the eNOS G894T polymorphism were significantly higher (p<0.001 and p<0.001, resp. in the HRS group than in both the stable cirrhosis (14.6%, 4.2%, and 11.5%, resp. and the control (22.0%, 2.0%, and 13.0%, resp. groups. Conclusion. The occurrence of mutant genotypes (GT/TT and mutant allele T in eNOS -G894T polymorphisms should be considered as a potential risk factor in cirrhotic patients with HRS.

  12. Antimicrobials in animal production: usage and practices among livestock farmers in Oyo and Kaduna States of Nigeria.

    Science.gov (United States)

    Ojo, Olufemi Ernest; Fabusoro, Eniola; Majasan, Ademola Adetokunbo; Dipeolu, Morenike Atinuke

    2016-01-01

    Antimicrobials have proven to be important for sustainable livestock production by their use as growth promoters and in the control of animal infections. However, injudicious use of antimicrobials could accelerate the emergence and spread of resistant bacterial strains with attendant socioeconomic and public health issues. This work assessed antimicrobial usage in animal production with emphasis on usage and practices by livestock producers in Oyo and Kaduna States of Nigeria. Data on antimicrobial usage were collected through interviews, questionnaire and focus group discussions. Four hundred and fifty-four farmers in 11 communities within 11 Local Government Areas of Oyo and Kaduna States of Nigeria were sampled in a multi-stage sampling procedure. The study showed that antimicrobial agents were widely distributed, readily accessible and commonly used in animal production. Fluoroquinolones and other critically important antimicrobials for human medicine were widely used in animals as prophylactics. Potentially harmful antimicrobials including furazolidones and chloramphenicol already banned for use in humans and animals were freely marketed and used in livestock production. Most of the respondents believed that veterinarians should be responsible for the administration of antimicrobials to animals, but in practice, they buy and administer antimicrobials without consulting veterinary professionals. It was observed that the ready availability of antimicrobial agents promoted the use of antimicrobials in livestock production and may encourage non-adherence to hygienic principles and management laxity in farm operations. The non-involvement of veterinary professionals and laboratory investigations in disease diagnosis prior to antimicrobial use could lead to improper usage that contribute to the development of antimicrobial resistance in bacterial strains. Responsible antimicrobial stewardship and strict regulations are vital to prolonging the benefits derivable from

  13. Achievement report on developing superconductor power applied technologies in fiscal 1999 (2). Research and development of superconductor wire materials, research and development of superconductor power generators, research of total systems, research and development of freezing systems, and verification tests; 1999 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 2. Chodendo senzai no kenkyu kaihatsu / chodendo hatsudenki no kenkyu kaihatsu / total system no kenkyu / reito system no kenkyu kaihatsu / jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    With an objective to achieve higher efficiency, higher density, and higher stability in power systems, research and development has been performed on superconductor power generators. This paper summarizes the achievements thereof in fiscal 1999. A verification test was given on the rotor of an ultra high speed responding generator. In a sudden short circuit test using the different phase charging method, no anomalies were found such as quench generation and vibration changes, wherein the healthiness of the generator was verified. In the VVVF actuation test, knowledge was acquired on the actuation method when the ultra high speed responding generator is applied to a combined cycle plant. After the verification test has been completed, the disassembly inspections such as visual check and non-destructive test were performed. With regard to the vacuum leakage found in the rotor under very low temperatures, the causes were presumed and the countermeasures were discussed by observing the weld structures. In the design research, the conception design on the 200-MW pilot generator was reviewed by reflecting the results of the verification tests on the model generator. At the same time, trial design was made on a 600-MW target generator. In summarizing the overall research achievements, the achievements and evaluations were summarized on technological issues that have been allotted to each research member. (NEDO)

  14. Aortic eNOS expression and phosphorylation in Apo-E knockout mice: differing effects of rapamycin and simvastatin.

    Science.gov (United States)

    Naoum, Joseph J; Zhang, Shu; Woodside, Kenneth J; Song, Wei; Guo, Qian; Belalcazar, Ligia M; Hunter, Glenn C

    2004-08-01

    The inhibition of nitric oxide (NO) by hypercholesterolemia may be mediated, in part, by interactions with caveolin-1 (Cav-1). Because of the facilitatory effects of statins on endothelial function and the adverse effects of rapamycin (RAPA) on plasma lipids, we compared the effects of simvastatin (SMV) and RAPA on endothelial NO synthase (eNOS) and Cav-1 protein expression and phosphorylation in the aortas of apolipoprotein E (Apo-E) knockout (-/-) mice. Apo-E -/- mice (n = 38) fed a high-cholesterol diet were given SMV (100 mg/kg/day po), RAPA (3 mg/kg/day ip), or no treatment for 10 weeks. Blood was drawn for serum lipid analysis, and protein was extracted for Western immunoblotting. Selected aortic specimens from 2 animals in each group were examined by histology and immunohistochemistry. The data are expressed as the mean +/- SEM and compared by the Student t test and ANOVA. Significance was established as P < .05. Lipid levels at 10 weeks were similar in the 3 groups except for higher triglyceride levels in RAPA-treated animals. eNOS expression was highest in RAPA-treated mice, but the p-eNOS to eNOS protein expression ratio was significantly greater in the SMV treatment group compared to both RAPA and controls (P < .05). Both Cav-1 and p-Cav-1 expression was significantly lower in the SMV-treated animals (P < .05) compared to mice treated with RAPA. Although eNOS expression was greatest in the RAPA-treated mice, the expression of p-eNOS was similar in the RAPA- and SMV-treated animals. The increase in eNOS induced by RAPA and the inverse relationship between p-eNOS and Cav-1 protein expression observed with SMV treatment suggest different mechanisms for the regulation of aortic eNOS expression in Apo-E mice by these 2 agents. Copyright 2004 Elsevier Inc.

  15. Sildenafil promotes eNOS activation and inhibits NADPH oxidase in the transgenic sickle cell mouse penis.

    Science.gov (United States)

    Musicki, Biljana; Bivalacqua, Trinity J; Champion, Hunter C; Burnett, Arthur L

    2014-02-01

    Sickle cell disease (SCD)-associated vasculopathy in the penis is characterized by aberrant nitric oxide and phosphodiesterase (PDE) 5 signaling, and by increased oxidative stress. Preliminary clinical trials show that continuous treatment with PDE5 inhibitor sildenafil unassociated with sexual activity decreases priapic activity in patients with SCD. However, the mechanism of its vasculoprotective effect in the penis remains unclear. We evaluated whether continuous administration of PDE5 inhibitor sildenafil promotes eNOS function at posttranslational levels and decreases superoxide-producing enzyme NADPH oxidase activity in the sickle cell mouse penis. SCD transgenic mice were used as an animal model of SCD. WT mice served as controls. Mice received treatment with the PDE5 inhibitor sildenafil (100 mg/kg/day) or vehicle for 3 weeks. eNOS phosphorylation on Ser-1177 (positive regulatory site), eNOS interactions with heat-shock protein 90 (HSP90) (positive regulator), phosphorylated AKT (upstream mediator of eNOS phosphorylation on Ser-1177), an NADPH oxidase catalytic subunit gp91(phox), and a marker of oxidative stress (4-hydroxy-2-nonenal [HNE]) were measured by Western blot. Effect of continuous sildenafil treatment on eNOS posttranslational activation, NADPH oxidase catalytic subunit, and oxidative stress in the penis of the sickle cell mouse. Continuous treatment with sildenafil reversed (P penis. Sildenafil treatment of WT mice did not affect any of these parameters. Our findings that sildenafil enhances eNOS activation and inhibits NADPH oxidase function in the sickle cell mouse penis offers a vasculoprotective molecular basis for the therapeutic effect of sildenafil in the penis in association with SCD. © 2013 International Society for Sexual Medicine.

  16. Fresh fish ( Clarias gariepinus ) marketing system in major towns of ...

    African Journals Online (AJOL)

    Fresh fish (Clarias gariepinus) marketing system in major towns of Ibarapa zone, Oyo State, Nigeria. A. O. Ayanboye, Z. O. Oluwafemi, R. A. Rafiu. Abstract. This study was carried out to investigate marketing system, market channel, nature of costs, market margins and the factors determining the marketing profit of fresh fish ...

  17. Prevalence and correlates of hypertension-outcome of a free medical screening in Oke-Ogun area of Oyo state, Nigeria, West Africa.

    Science.gov (United States)

    Shittu, Rasaki O; Odeigah, Louis O; Fakorede, Kasali O; Sikiru, Biliaminu A; Sule, Abdullateef G; Musah, Yusuf; Adeyemi, Folorunsho M

    2018-02-01

    Literature abounds on prevalence of hypertension in Nigeria and urban areas of Oyo state but none in Oke-Ogun geopolitical zone, which constitutes 10 local governments of the 33 in Oyo state, despite the fact that they have high genetic and environmental predisposition to developing high blood pressure. The purpose of the study was to determine the prevalence of high blood pressure and associated risk factors among indigenes of Oke-ogun, Oyo state. A total of 10,000 respondents were recruited using proportionate sampling techniques. Hypertension was defined according to Joint National Committee-8 criteria. The fasting plasma glucose of the respondents were classified as normal (≤6 mmol/L), prediabetes (6.1-6.9 mmol/L), and diabetes (≥7 mmol/L). Body mass index was calculated as weight (kg)/height (m 2 ) and classified as malnutrition/underweight (state. Their low socioeconomic status, low educational background, malnutrition, and genetic predisposition were identified risk factors. Copyright © 2018 American Society of Hypertension. Published by Elsevier Inc. All rights reserved.

  18. Achievement report for fiscal 1998 on the development of superconductor power application technology. 2. Research and development of superconducting wire and superconductive power generator, research of total system, research and development of refrigeration system, and verification test; 1998 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 2. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total sytsem no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    The slow excitation response type power generator is studied when the rotor and stator of a 70,000kW-class model are combinedly subjected to an on-site verification test, when a good result is obtained. The rotor is disassembled for inspection, and its members are found to be sound without any problem in terms of mechanical strength. The quick excitation response type is studied when a 70,000kW model is experimentally built and subjected to an on-site verification test after a rotation and excitation test in the factory, when the pilot machine concept design is reviewed. In the study of a total system, efforts continue for the review of the model machine test method, improvement on generator design and analytical methods, development of operating methods, and the effect of its introduction into the power system. Since a He-refrigerated system is requested to exhibit high reliability for application to power equipment and to be capable of continuous long-period operation, a system having constituents with their reliability enhanced and an appropriate redundant system is developed, and a verification study is under way which will continue for more than 10,000 hours. Described also is an oil-free low-temperature turbo refrigerator. The latest quick excitation response type rotor is also tested for verification. (NEDO)

  19. Partial deletion of eNOS gene causes hyperinsulinemic state, unbalance of cardiac insulin signaling pathways and coronary dysfunction independently of high fat diet.

    Directory of Open Access Journals (Sweden)

    Cecilia Vecoli

    Full Text Available Abnormalities in eNOS gene, possibly interacting with high fat diet (HFD, affect peripheral vascular function and glucose metabolism. The relative role of eNOS gene, HFD and metabolic derangement on coronary function has not been fully elucidated. We test whether eNOS gene deficiency per se or in association with HFD modulates coronary function through mechanisms involving molecular pathways related to insulin signaling. Wild type (WT, eNOS-/- and eNOS+/- mice were studied. WT and eNOS+/- mice were fed with either standard or HF diet for 16 weeks and compared with standard diet fed eNOS-/-. Glucose and insulin tolerance tests were performed during the last week of diet. Coronary resistance (CR was measured at baseline and during infusions of acetylcholine (Ach or sodium-nitroprusside (SNP to evaluate endothelium-dependent or independent vasodilation, in the Langendorff isolated hearts. Cardiac expression of Akt and ERK genes as evaluation of two major insulin-regulated signaling pathways involved in the control of vascular tone were assessed by western blot. HFD-fed mice developed an overt diabetic state. Conversely, chow-fed genetically modified mice (in particular eNOS-/- showed a metabolic pattern characterized by normoglycemia and hyperinsulinemia with a limited degree of insulin resistance. CR was significantly higher in animals with eNOS gene deletions than in WT, independently of diet. Percent decrease in CR, during Ach infusion, was significantly lower in both eNOS-/- and eNOS+/- mice than in WT, independently of diet. SNP reduced CR in all groups except eNOS-/-. The cardiac ERK1-2/Akt ratio, increased in animals with eNOS gene deletions compared with WT, independently of diet. These results suggest that the eNOS genetic deficiency, associated or not with HFD, has a relevant effect on coronary vascular function, possibly mediated by increase in blood insulin levels and unbalance in insulin-dependent signaling in coronary vessels

  20. Investigation into The Effect of Arabic Incompetence on The Students’ Performance in Islamic Studies in Ibadan South-East Of Oyo State In Nigeria

    Directory of Open Access Journals (Sweden)

    Kazeem Adekunle Adegoke

    2016-11-01

    Full Text Available Abstract This study investigated the effect of incompetence in Arabic language on students’ performances in Islamic studies in the selected senior secondary schools in Ibadan South-East Local Government Area of Oyo State, Nigeria. The main objective of this study was to ascertain the argument that the poor performance of senior secondary students in Islamic studies is as a result of incompetence and non-proficiency in Arabic language on the side of learners and teachers. The methodology employed in this study was the quantitative approach. Location of the study was Ibadan South-East Local Government Area of Oyo State. Teachers of Islamic studies and students were randomly selected using the stratified random sampling technique from six senior secondary schools. The data gathered were analysed using simple percentages and chi-square statistical analysis. The findings revealed that significant number of students in Senior Secondary School’s poor performance in Islamic studies was due to their incompetence in Arabic as a result of their lack of exposure to the Arabic language and their poor background in Arabic from the previous classes. It also revealed that some of their teachers in Islamic studies were not competent in Arabic reading and writing. Abstrak Penelitian ini menginvestigasi efek ketidakmampuan bahasa Arab terhadap kinerja siswa studi Islam di sekolah-sekolah menengah atas terpilih di Ibadan Tenggara, di Area Pemerintahan Daerah Negara Oyo, Nigeria. Tujuan utama penelitian ini untuk menegaskan argumen bahwa buruknya kinerja siswa sekolah menengah atas pada studi Islam adalah akibat dari ketidakmampuan dan ketidakmahiran bahasa Arab pada peserta didik dan guru. Metode penelitian pendekatan kuantitatif. Lokasi penelitian adalah di Area Pemerintah Daerah Ibadan Tenggara Negara Oyo. Guru dan siswa program studi Islam dipilih secara acak menggunakan teknik stratified random sampling dari enam sekolah menengah atas. Data yang terkumpul

  1. Vasoinhibins Prevent Bradykinin-Stimulated Endothelial Cell Proliferation by Inactivating eNOS via Reduction of both Intracellular Ca2+ Levels and eNOS Phosphorylation at Ser1179

    Science.gov (United States)

    Thebault, Stéphanie; González, Carmen; García, Celina; Zamarripa, David Arredondo; Nava, Gabriel; Vaca, Luis; López-Casillas, Fernando; de la Escalera, Gonzalo Martínez; Clapp, Carmen

    2011-01-01

    Vasoinhibins, a family of antiangiogenic peptides derived from prolactin proteolysis, inhibit the vascular effects of several proangiogenic factors, including bradykinin (BK). Here, we report that vasoinhibins block the BK-induced proliferation of bovine umbilical vein endothelial cells. This effect is mediated by the inactivation of endothelial nitric oxide synthase (eNOS), as the NO donor DETA-NONOate reverted vasoinhibin action. It is an experimentally proven fact that the elevation of intracellular Ca2+ levels ([Ca2+]i) upon BK stimulation activates eNOS, and vasoinhibins blocked the BK-mediated activation of phospholipase C and the formation of inositol 1,4,5-triphosphate leading to a reduced release of Ca2+ from intracellular stores. The [Ca2+]i rise evoked by BK also involves the influx of extracellular Ca2+ via canonical transient receptor potential (TRPC) channels. Vasoinhibins likely interfere with TRPC-mediated Ca2+ entry since La3+, which is an enhancer of TRPC4 and TRPC5 channel activity, prevented vasoinhibins from blocking the stimulation by BK of endothelial cell NO production and proliferation, and vasoinhibins reduced the BK-induced increase of TRPC5 mRNA expression. Finally, vasoinhibins prevented the BK-induced phosphorylation of eNOS at Ser1179, a post-translational modification that facilitates Ca2+-calmodulin activation of eNOS. Together, our data show that vasoinhibins, by lowering NO production through the inhibition of both [Ca2+]i mobilization and eNOS phosphorylation, prevent the BK-induced stimulation of endothelial cell proliferation. Thus, vasoinhibins help to regulate BK effects on angiogenesis and vascular homeostasis.

  2. Vasoinhibins Prevent Bradykinin-Stimulated Endothelial Cell Proliferation by Inactivating eNOS via Reduction of both Intracellular Ca2+ Levels and eNOS Phosphorylation at Ser1179

    Directory of Open Access Journals (Sweden)

    Carmen Clapp

    2011-07-01

    Full Text Available Vasoinhibins, a family of antiangiogenic peptides derived from prolactin proteolysis, inhibit the vascular effects of several proangiogenic factors, including bradykinin (BK. Here, we report that vasoinhibins block the BK-induced proliferation of bovine umbilical vein endothelial cells. This effect is mediated by the inactivation of endothelial nitric oxide synthase (eNOS, as the NO donor DETA-NONOate reverted vasoinhibin action. It is an experimentally proven fact that the elevation of intracellular Ca2+ levels ([Ca2+]i upon BK stimulation activates eNOS, and vasoinhibins blocked the BK-mediated activation of phospholipase C and the formation of inositol 1,4,5-triphosphate leading to a reduced release of Ca2+ from intracellular stores. The [Ca2+]i rise evoked by BK also involves the influx of extracellular Ca2+ via canonical transient receptor potential (TRPC channels. Vasoinhibins likely interfere with TRPC-mediated Ca2+ entry since La3+, which is an enhancer of TRPC4 and TRPC5 channel activity, prevented vasoinhibins from blocking the stimulation by BK of endothelial cell NO production and proliferation, and vasoinhibins reduced the BK-induced increase of TRPC5 mRNA expression. Finally, vasoinhibins prevented the BK-induced phosphorylation of eNOS at Ser1179, a post-translational modification that facilitates Ca2+-calmodulin activation of eNOS. Together, our data show that vasoinhibins, by lowering NO production through the inhibition of both [Ca2+]i mobilization and eNOS phosphorylation, prevent the BK-induced stimulation of endothelial cell proliferation. Thus, vasoinhibins help to regulate BK effects on angiogenesis and vascular homeostasis.

  3. Triterpenoic Acids from Apple Pomace Enhance the Activity of the Endothelial Nitric Oxide Synthase (eNOS).

    Science.gov (United States)

    Waldbauer, Katharina; Seiringer, Günter; Nguyen, Dieu Linh; Winkler, Johannes; Blaschke, Michael; McKinnon, Ruxandra; Urban, Ernst; Ladurner, Angela; Dirsch, Verena M; Zehl, Martin; Kopp, Brigitte

    2016-01-13

    Pomace is an easy-accessible raw material for the isolation of fruit-derived compounds. Fruit consumption is associated with health-promoting effects, such as the prevention of cardiovascular disease. Increased vascular nitric oxide (NO) bioavailability, for example, due to an enhanced endothelial nitric oxide synthase (eNOS) activity, could be one molecular mechanism mediating this effect. To identify compounds from apple (Malus domestica Borkh.) pomace that have the potential to amplify NO bioavailability via eNOS activation, a bioassay-guided fractionation of the methanol/water (70:30) extract has been performed using the (14)C-L-arginine to (14)C-L-citrulline conversion assay (ACCA) in the human endothelium-derived cell line EA.hy926. Phytochemical characterization of the active fractions was performed using the spectrophotometric assessment of the total phenolic content, as well as TLC, HPLC-DAD-ELSD, and HPLC-MS analyses. Eleven triterpenoic acids, of which one is a newly discovered compound, were identified as the main constituents in the most active fraction, accompanied by only minor contents of phenolic compounds. When tested individually, none of the tested compounds exhibited significant eNOS activation. Nevertheless, cell stimulation with the reconstituted compound mixture restored eNOS activation, validating the potential of apple pomace as a source of bioactive components.

  4. Impact of Lifestyle Intervention on HDL-Induced eNOS Activation and Cholesterol Efflux Capacity in Obese Adolescent

    Directory of Open Access Journals (Sweden)

    Jenny Wesnigk

    2016-01-01

    Full Text Available Background. Endothelial dysfunction occurs in obese children and adolescent and is regarded as a key step in the development of atherosclerosis. Important components for the development of endothelial dysfunction are reduced activity of endothelial nitric oxide synthase (eNOS and an increase in cholesterol deposition in the vessel wall, due to reduced reverse cholesterol transport (RCT activity. High density lipoprotein (HDL exhibits antiatherosclerotic properties including modulation of eNOS activity and cholesterol efflux capacity. Lifestyle intervention programs can modify endothelial dysfunction in obese adolescents, but their impact on HDL-mediated eNOS activation and RCT is unknown so far. Methods. Obese adolescents (15±1 years, BMI > 35 kg/m2 where randomized either to an intervention group (IG, n=8; restricted diet and exercise or to a usual care group (UC, n=8. At the beginning and after 10 months of treatment HDL-mediated eNOS phosphorylation and cholesterol efflux capacity were evaluated. Results. Ten months of treatment resulted in a substantial weight loss (−31%, an improvement of endothelial function, and an increase in HDL-mediated eNOS-Ser1177 phosphorylation and RCT. A correlation between change in eNOS-Ser1177 phosphorylation or RCT and change in endothelial function was noted. Conclusion. A structured lifestyle intervention program improves antiatherosclerotic HDL functions, thereby positively influencing endothelial function.

  5. Effect of resistance training on microvascular density and eNOS content in skeletal muscle of sedentary men.

    Science.gov (United States)

    Cocks, Matthew; Shaw, Christopher S; Shepherd, Sam O; Fisher, James P; Ranasinghe, Aaron M; Barker, Thomas A; Tipton, Kevin D; Wagenmakers, Anton J M

    2014-11-01

    The effects of RT on muscle mass, strength, and insulin sensitivity are well established, but the underlying mechanisms are only partially understood. The main aim of this study was to investigate whether RT induces changes in endothelial enzymes of the muscle microvasculature, which would increase NO bioavailability and could contribute to improved insulin sensitivity. Eight previously sedentary males (age 20 ± 0.4 years, BMI 24.5 ± 0.9 kg/m(2) ) completed six weeks of RT 3x/week. Muscle biopsies were taken from the m. vastus lateralis and microvascular density; and endothelial-specific eNOS content, eNOS Ser(1177) phosphorylation, and NOX2 content were assessed pre- and post-RT using quantitative immunofluorescence microscopy. Whole-body insulin sensitivity (measured as Matsuda Index), microvascular Kf (functional measure of the total available endothelial surface area), and arterial stiffness (AIx, central, and pPWV) were also measured. Measures of microvascular density, microvascular Kf , microvascular eNOS content, basal eNOS phosphorylation, and endothelial NOX2 content did not change from pre-RT to post-RT. RT increased insulin sensitivity (p < 0.05) and reduced resting blood pressure and AIx (p < 0.05), but did not change central or pPWV. RT did not change any measure of muscle microvascular structure or function. © 2014 John Wiley & Sons Ltd.

  6. [The action of PI3K/AKT during genistein promoting the activity of eNOS].

    Science.gov (United States)

    Zheng, Feng-li; Zhao, Jia-hui; Zhang, Hua-ping

    2012-04-01

    Genistein could inhibit the development of atherosclerosis. This study explored the role of PI3K/AKT signaling during genistein promoted eNOS activation. Human umbilical vein endothelial cells (HUVECs) were incubated with ox-LDL (100 mg/L), then treated with genistein (100 nmol/L) for 5, 10, 15, 30 and 60 min. The production of NO was assessed by Griess reaction in cell culture supernatant. The mRNA expression of endothelial nitric oxide synthase (eNOS) was detected by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of eNOS and phosphorylation eNOS(Ser(1179)) were determined by Western blot. The effect of genistein on phosphorylation eNOS(Ser(1179)) level was also observed in the presence of LY294002 or NSC154020 (PI3K and AKT inhibitors). The concentration of NO and the expression level of phosphorylation eNOS(Ser(1179)) were significantly increased in ox-LDL + genistein treated cells than ox-LDL treated cells (all P 0.05). Furthermore, the expression level of phosphorylation eNOS(Ser(1179)) was significantly lower in PIK3/AKT inhibitors LY294002 and NSC154020 treated cells compared with ox-LDL + genistein treated cells (all P Genistein could promote the activity of eNOS through increasing phosphorylation eNOS(Ser(1179)) level through PI3K/AKT pathway.

  7. Mesenchymal Stem Cells with eNOS Over-Expression Enhance Cardiac Repair in Rats with Myocardial Infarction.

    Science.gov (United States)

    Chen, Leilei; Zhang, Yuan; Tao, Liangliang; Yang, Zhijian; Wang, Liansheng

    2017-02-01

    Transplantation of mesenchymal stem cells (MSCs) is a promising therapeutic option for patients with acute myocardial infarction. We show here that the ectopic overexpression of endothelial nitric oxide synthases (eNOS), an endothelial form of NOS, could enhance the ability of MSCs in treating ischemic heart damage after the occlusion of the coronary artery. Adenoviral delivery of human eNOS gene into mouse bone marrow-derived MSCs (BM-MSCs) conferred resistance to oxygen glucose deprivation (OGD)-induced cell death in vitro, and elevated the bioavailability of nitric oxide when injected into the myocardium in vivo. In a rat model of acute myocardial infarction, the transplantation of eNOS-overexpressing BM-MSCs significantly reduced myocardial infarct size, corrected hemodynamic parameters and increased capillary density. We also found that the synergistic effects were consistently better than either treatment alone. These findings reveal a positive role of elevated eNOS expression in cardiac repair, and suggest the combination of eNOS and MSC transplant therapy as a potential approach for treating myocardial infarction.

  8. Effects of hyperbaric oxygen therapy in enhancing expressions of e-NOS, TNF-α and VEGF in wound healing

    Science.gov (United States)

    Susilo, Imam; Devi, Anita; Purwandhono, Azham; Hadi Warsito, Sunaryo

    2017-05-01

    Wound healing is a physiological process that occurs progressively through overlapping phases. Tissue oxygenation is an important part of the complex regulation for wound healing. Hyperbaric Oxygen (HBO) therapy is a method of increasing oxygen delivery to tissues. The therapy improves tissue oxygenation and stimulates the formation of H2O2 as a secondary messenger for Tumour Necrosis Factor alpha (TNF α), e-NOS, VEGF and Nuclear Factor Kappa Beta phosphorylation (NF-Kb) which play an important role in the rapid transcription of a wide variety of genes in response to extracellular stimuli. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of e-NOS, TNF-α, VEGF and wound healing. This study is an animal study with a ‘randomized control group of pre-test and post test design’ on 28 Wistar rats. Randomly, the rats were divided into 4 groups with 7 rats in each group. The HBO treatment group 1 received 5 sessions of HBO 2.4 ATA in 3 × 30 minutes; the HBO treatment group 2 received 10 sessions of HBO 2.4 ATA in 3 × 30 minutes; and each of the control groups were without HBO. Each of the 28 male rats were given a full thickness excisional wound of 1 × 1cm. Examinations of e-NOS, TNF-α, VEGF expressions and wound healing were performed on day-0 (pre-HBO) and day-5 HBO or on day-0 (pre-HBO) and day-10 HBO. The resultsshowthat the Hyperbaric Oxygen therapy can improve e-NOS (p=0.02), TNF-α (p= 0.02), VEGF expression (p=0.02) and wound healing (p=0.002) significantly in the provision of HBO 2.4 ATA for 3 × 30 minutes in 5 sessions over 5 consecutive days. While the 10 sessions of HBO 2.4 ATA for 3 × 30 minutes over 10 consecutive days only increase e-NOS (p=0.02), TNF-α (p=0.04), VEGF expression significantly (p=0.03) but do not improve wound healing significantly (p=0.3) compared with no HBO. The study concludes that HBO can improve the expressions of e-NOS, TNF-α, VEGF and wound healing in the provision of HBO

  9. Screening of antibiotics and chemical analysis of penicillin residue in fresh milk and traditional dairy products in Oyo state, Nigeria.

    Science.gov (United States)

    Olatoye, Isaac Olufemi; Daniel, Oluwayemisi Folashade; Ishola, Sunday Ayobami

    2016-09-01

    There are global public health and economic concerns on chemical residues in food of animal origin. The use of antibiotics in dairy cattle for the treatment of diseases such as mastitis has contributed to the presence of residues in dairy products. Penicillin residues as low as 1 ppb can lead to allergic reactions and shift of resistance patterns in microbial population as well as interfere with the processing of several dairy products. Antibiotic monitoring is an essential quality control measure in safe milk production. This study was aimed at determining antibiotic residue contamination and the level of penicillin in dairy products from Fulani cattle herds in Oyo State. The presence of antibiotic residues in 328 samples of fresh milk, 180 local cheese (wara), and 90 fermented milk (nono) from Southwest, Nigeria were determined using Premi® test kit (R-Biopharm AG, Germany) followed by high-performance liquid chromatography analysis of penicillin-G residue. Antibiotic residues were obtained in 40.8%, 24.4% and 62.3% fresh milk, wara and nono, respectively. Penicillin-G residue was also detected in 41.1% fresh milk, 40.2% nono and 24.4% wara at mean concentrations of 15.22±0.61, 8.24±0.50 and 7.6±0.60 μg/L with 39.3%, 36.7% and 21.1%, respectively, containing penicillin residue above recommended Codex maximum residue limit (MRL) of 5 μg/L in dairy. There was no significant difference between the mean penicillin residues in all the dairy products in this study. The results are of food safety concern since the bulk of the samples and substantial quantities of dairy products in Oyo state contained violative levels of antibiotic residues including penicillin residues in concentrations above the MRL. This could be due to indiscriminate and unregulated administration of antibiotics to dairy cattle. Regulatory control of antibiotic use, rapid screening of milk and dairy farmers' extension education on alternatives to antibiotic prophylaxis, veterinary prescriptions

  10. Screening of antibiotics and chemical analysis of penicillin residue in fresh milk and traditional dairy products in Oyo state, Nigeria

    Directory of Open Access Journals (Sweden)

    Isaac Olufemi Olatoye

    2016-09-01

    Full Text Available Background and Aim: There are global public health and economic concerns on chemical residues in food of animal origin. The use of antibiotics in dairy cattle for the treatment of diseases such as mastitis has contributed to the presence of residues in dairy products. Penicillin residues as low as 1 ppb can lead to allergic reactions and shift of resistance patterns in microbial population as well as interfere with the processing of several dairy products. Antibiotic monitoring is an essential quality control measure in safe milk production. This study was aimed at determining antibiotic residue contamination and the level of penicillin in dairy products from Fulani cattle herds in Oyo State. Materials and Methods: The presence of antibiotic residues in 328 samples of fresh milk, 180 local cheese (wara, and 90 fermented milk (nono from Southwest, Nigeria were determined using Premi® test kit (R-Biopharm AG, Germany followed by high-performance liquid chromatography analysis of penicillin-G residue. Results: Antibiotic residues were obtained in 40.8%, 24.4% and 62.3% fresh milk, wara and nono, respectively. Penicillin-G residue was also detected in 41.1% fresh milk, 40.2% nono and 24.4% wara at mean concentrations of 15.22±0.61, 8.24±0.50 and 7.6±0.60 μg/L with 39.3%, 36.7% and 21.1%, respectively, containing penicillin residue above recommended Codex maximum residue limit (MRL of 5 μg/L in dairy. There was no significant difference between the mean penicillin residues in all the dairy products in this study. Conclusion: The results are of food safety concern since the bulk of the samples and substantial quantities of dairy products in Oyo state contained violative levels of antibiotic residues including penicillin residues in concentrations above the MRL. This could be due to indiscriminate and unregulated administration of antibiotics to dairy cattle. Regulatory control of antibiotic use, rapid screening of milk and dairy farmers

  11. Uncoupling of eNOS contributes to redox-sensitive leukocyte recruitment and microvascular leakage elicited by methylglyoxal.

    Science.gov (United States)

    Su, Yang; Qadri, Syed M; Hossain, Mokarram; Wu, Lingyun; Liu, Lixin

    2013-12-15

    Elevated levels of the glycolysis metabolite methylglyoxal (MG) have been implicated in impaired leukocyte-endothelial interactions and vascular complications in diabetes, putative mechanisms of which remain elusive. Uncoupling of endothelial nitric oxide synthase (eNOS) was shown to be involved in endothelial dysfunction in diabetes. Whether MG contributes to these effects has not been elucidated. By using intravital microscopy in vivo, we demonstrate that MG-triggered reduction in leukocyte rolling velocity and increases in rolling flux, adhesion, emigration and microvascular permeability were significantly abated by scavenging reactive oxygen species (ROS). In murine cremaster muscle, MG treatment reduced tetrahydrobiopterin (BH4)/total biopterin ratio, increased arginase expression and stimulated ROS and superoxide production. The latter was significantly blunted by ROS scavengers Tempol (300μM) or MnTBAP (300μM), by BH4 supplementation (100μM) or by NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 20μM). In these tissues and cultured murine and human primary endothelial cells, MG increased eNOS monomerization and decreased BH4/total biopterin ratio, effects that were significantly mitigated by supplementation of BH4 or its precursor sepiapterin but not by L-NAME or tetrahydroneopterin, indicative of MG-triggered eNOS uncoupling. MG treatment further decreased the expression of guanosine triphosphate cyclohydrolase I in murine primary endothelial cells. MG-induced leukocyte recruitment was significantly attenuated by supplementation of BH4 or sepiapterin or suppression of superoxide by L-NAME confirming the role of eNOS uncoupling in MG-elicited leukocyte recruitment. Together, our study uncovers eNOS uncoupling as a pivotal mechanism in MG-induced oxidative stress, microvascular hyperpermeability and leukocyte recruitment in vivo. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Detrimental effect of combined exercise training and eNOS overexpression on cardiac function after myocardial infarction.

    Science.gov (United States)

    de Waard, Monique C; van der Velden, Jolanda; Boontje, Nicky M; Dekkers, Dick H W; van Haperen, Rien; Kuster, Diederik W D; Lamers, Jos M J; de Crom, Rini; Duncker, Dirk J

    2009-05-01

    It has been reported that exercise after myocardial infarction (MI) attenuates left ventricular (LV) pump dysfunction by normalization of myofilament function. This benefit could be due to an exercise-induced upregulation of endothelial nitric oxide synthase (eNOS) expression and activity. Consequently, we first tested the hypothesis that the effects of exercise after MI can be mimicked by elevated eNOS expression using transgenic mice with overexpression of human eNOS (eNOSTg). Both exercise and eNOSTg attenuated LV remodeling and dysfunction after MI in mice and improved cardiomyocyte maximal force development (F(max)). However, only exercise training restored myofilament Ca(2+)-sensitivity and sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA)2a protein levels and improved the first derivative of LV pressure at 30 mmHg. Conversely, only eNOSTg improved survival. In view of these partly complementary actions, we subsequently tested the hypothesis that combining exercise and eNOSTg would provide additional protection against LV remodeling and dysfunction after MI. Unexpectedly, the combination of exercise and eNOSTg abolished the beneficial effects on LV remodeling and dysfunction of either treatment alone. The latter was likely due to perturbations in Ca(2+) homeostasis, as myofilament F(max) actually increased despite marked reductions in the phosphorylation status of several myofilament proteins, whereas the exercise-induced increases in SERCA2a protein levels were lost in eNOSTg mice. Antioxidant treatment with N-acetylcysteine or supplementation of tetrahydrobiopterin and l-arginine prevented these detrimental effects on LV function while partly restoring the phosphorylation status of myofilament proteins and further enhancing myofilament F(max). In conclusion, the combination of exercise and elevated eNOS expression abolished the cardioprotective effects of either treatment alone after MI, which appeared to be, at least in part, the result of increased

  13. Influence of core self-evaluation and reward system on ...

    African Journals Online (AJOL)

    This study investigated the influence of core self-evaluation (self efficacy, self esteem, work locus of control and neuroticism) and reward system on career commitment of local government employees in Oyo State, Nigeria. Descriptive survey research design was adopted in the study and using stratified random of equal ...

  14. SEROLOGICAL SURVEY OF TOXOPLASMOSIS, NEOSPOROSIS AND BRUCELLOSIS AMONG CATTLE HERDS IN OYO STATE, SOUTH-WESTERN NIGERIA.

    Science.gov (United States)

    Ayinmode, Adekunle; Akinseye, Victor; Schares, Gereon; Cadmus, Simeon

    2017-01-01

    Several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. This study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in Oyo State, southwest Nigeria. A cross-sectional survey to screen for antibodies to Toxoplasma gondii , Neospora caninum and Brucella abortus was conducted among 174 cattle in 17 herds. Sera obtained from the cattle were screened for antibodies to Toxoplasma gondii and Neospora caninum using enzyme-linked immunosorbent assay (ELISA) and for Brucella abortus antibodies using Rose Bengal test and Competitive Enzyme Linked Immunosorbent Assay (cELISA). Overall, herd level prevalence of 52.9%, 23.5% and 23.5% as well as individual prevalence of 7.5%, 3.4% and 3.4% was obtained for toxoplasmosis, neosporosis and brucellosis, respectively. Antibodies to T. gondii , N. caninum and B. abortus were detected in 2 of the 17 herds, T. gondii and N. caninum in 4 herds, and T. gondii and B. abortus in 4 herds. Statistically significant association was only found between seropositivity to T. gondii antibodies and sex (pneosporosis and brucellosis are prevalent among cattle herds screened in the study area. Considering the potential impact of these diseases on livestock management and production, extensive surveillance is necessary for development and implementation of effective control and prevention strategies.

  15. International note: awareness and context of cyber-harassment among secondary school students in Oyo state, Nigeria.

    Science.gov (United States)

    Olumide, Adesola O; Adams, Patricia; Amodu, Olukemi K

    2015-02-01

    We determined the awareness and context of cyber-harassment among secondary school students (653 survey respondents and 18 in-depth interviewees) in Oyo state, Nigeria. Respondents' mean age was 14.2 ± 2.2 years and 53.9% were aware of cyber-harassment occurring in their school or among their friends. Cyber-harassment was often perpetrated via phone calls (62.5%), text messaging (36.9%), chat rooms (28.7%), through pictures or video clips sent via mobile phones (11.9%), emails (6.8%) or websites (5.9%). Cyber-harassment behaviours mentioned were the use of abusive words (25.4%), saying mean things or making fun of the victim (13.9%), solicitations for relationships (7.9%) or sex (6.8%) and spreading rumours about the victim (6.8%). In-depth interviewees recounted experiences of cyber-harassment suffered by their friends. Many were relationship-related, sexual solicitations and threats and corroborated quantitative findings. Respondents are aware of cyber-harassment occurring among students in the study area. Comprehensive interventions to address the problem need to be instituted. Copyright © 2014 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

  16. Acceptance and Utilisation of Sulphadoxine-Pyrimethamine and Insecticide-Treated Nets among Pregnant Women in Oyo State, Nigeria.

    Science.gov (United States)

    Adeola, Aderonke A; Okwilagwe, Eugenia A

    2015-01-01

    The study is an investigation of the acceptance and utilisation of Sulphadoxine-Pyrimethamine (Fansidar), the drug of choice for Intermittent Preventive Treatment in pregnancy, and Insecticide-Treated Nets among pregnant women who access different health facilities in Oyo State, Nigeria. Pregnant women (582) attending government primary healthcare antenatal clinics and 50 attending faith clinics purposively selected responded to structured instruments that were analysed using percentages, t-test correlation, and multiple regression. Acceptance and utilisation of RBM tools were higher in government clinics than faith clinics and in rural areas. Pregnant women in government clinics, 60.8% and 66.8%, and faith clinics, 18% and 38.0%, utilised Roll Back Malaria tools, significant at t (630) = 5.81, p ≤ 0.05, and t (630) = 3.99, p ≤ 0.05, respectively. Pregnant women in rural locations who accessed government clinics utilised Roll Back Malaria tools more than those in urban areas, t (580) = -641, p ≤ 0.05. Number of pregnancies, educational qualification of the pregnant women, and marital status significantly and consistently influenced acceptance and utilisation of these tools. To ensure that set targets are met, the utilization of RBM tools among the two categories of pregnant women can be improved by increasing the supply of the tools and ensuring that treatment is free.

  17. Effect of brain-based learning strategy on students achievement in senior secondary school mathematics in Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Samuel Adejare Awolola

    2011-06-01

    Full Text Available One dominant factor on how well students learn mathematics is the quality of teaching. Studies have shown that typical mathematics classroom is frosted with teaching technique that centered on explain – practice – memorize. There is a paucity particularly in Nigeria. This study therefore, investigated the effect of brain-based learning strategy on the achievement regarding the learning of Mathematics of 522 Senior Secondary School Students in Oyo State, Nigeria. The moderator effect of cognitive style was also examined on independent variable (instructional strategy and dependent variable (mathematics achievement. The study adopted a pretest-posttest non-equivalent control group design in a quasi – experimental setting. The ANCOVA statistic was used to analyzed the data collected fro the study. The result revealed significant main effect of treatment, (F(1,510 = 75.0; P < 0.05, cognitive style (F(1,510 = 23.78; P < 0.05 and significant interaction effect of treatment and cognitive style (F(1,510 = 5.027; P < 0.05 on achievement in mathematics. The result showed that brain-based instructional strategy enhanced students’ achievement in mathematics more than the conventional lecture method. It is therefore recommended that Teachers of mathematics should adopt the strategy in teaching mathematics in senior secondary school.

  18. Knowledge, Attitude, and Preventive Practices among Prison Inmates in Ogbomoso Prison at Oyo State, South West Nigeria

    Directory of Open Access Journals (Sweden)

    Abdulsalam Saliu

    2014-01-01

    Full Text Available Prisoners are at special risk for infection with human immunodeficiency virus (HIV because of overcrowded prisons, unprotected sex and sexual assault, occurrence of sexual practices that are risky to health, unsafe injecting practices, and inadequate HIV prevention, care, and support services. This study aimed to describe the knowledge, attitude, and preventive practices towards HIV/AIDS by male inmates in Ogbomoso Prison at Oyo State, South West Nigeria. This was a cross-sectional study. A simple random sampling method was employed to select 167 male participants and data were collected using pretested structured interviewer-administered questionnaire. The data were collated and analyzed using the Statistical Package for Social Sciences version 17. Fifty (29.9% were in the age group 20–24 years with mean age of 30.99±11.41. About half (50.3% had been married before incarceration. Family and friends (30%, health care workers (25%, prison staff (20%, and mass media (25% were the commonest sources of information on HIV/AIDS. Knowledge about HIV was found to be high (94.6%. About 68.9% believed that people with the disease should be avoided. The knowledge about HIV/AIDS among inmates was high, but misconceptions about HIV/AIDS are still rife among the prisoners and educational programs would be required to correct this.

  19. Knowledge, Attitude, and Preventive Practices among Prison Inmates in Ogbomoso Prison at Oyo State, South West Nigeria.

    Science.gov (United States)

    Saliu, Abdulsalam; Akintunde, Babatunde

    2014-01-01

    Prisoners are at special risk for infection with human immunodeficiency virus (HIV) because of overcrowded prisons, unprotected sex and sexual assault, occurrence of sexual practices that are risky to health, unsafe injecting practices, and inadequate HIV prevention, care, and support services. This study aimed to describe the knowledge, attitude, and preventive practices towards HIV/AIDS by male inmates in Ogbomoso Prison at Oyo State, South West Nigeria. This was a cross-sectional study. A simple random sampling method was employed to select 167 male participants and data were collected using pretested structured interviewer-administered questionnaire. The data were collated and analyzed using the Statistical Package for Social Sciences version 17. Fifty (29.9%) were in the age group 20-24 years with mean age of 30.99 ± 11.41. About half (50.3%) had been married before incarceration. Family and friends (30%), health care workers (25%), prison staff (20%), and mass media (25%) were the commonest sources of information on HIV/AIDS. Knowledge about HIV was found to be high (94.6%). About 68.9% believed that people with the disease should be avoided. The knowledge about HIV/AIDS among inmates was high, but misconceptions about HIV/AIDS are still rife among the prisoners and educational programs would be required to correct this.

  20. PSYCHOSOCIAL INFLUENCE OF HEARING IMPAIRMENT ON THE INTERPERSONAL BEHAVIOR OF YOUTHS WITH HEARING IMPAIRMENT IN OYO STATE, NIGERIA

    Directory of Open Access Journals (Sweden)

    Osisanya AYO

    2013-03-01

    Full Text Available Individuals with hearing impairment are confronted with a lot of problems due to the condition of their disability. This has a negative impact on their social and psychological well-being with multiplying effect on their interpersonal relationship. Therefore, this study investigated the psycho-social influence of hearing impairment on interpersonal behavior of youths with hearing loss.MethodologyThe study adopted a survey research design. A sample consisting of 211 participants with hearing loss were purposively selected from the Federal College of Education (Special Oyo, Nigeria. A questionnaire, part of Psycho-social Competence Scale (PCS, was used for data collection with reliability coefficient of 0.72.ResultsThe findings revealed that hearing impairement affects social interaction of youths with hearing impairment, hearing loss affects emotional well-being of youths with hearing impairment and youths with hearing impairment feel inferior in company of persons without hearing impairment. Based on this, it was recommended that a friendly home environment should be made and youths with hearing impairment should be advised to accept their loss and take it as a challenge that can be used to achieve a better end and the society should have right attitude and beliefs toward youths with hearing impairment.

  1. Identification and Function Analysis of enolase Gene NlEno1 from Nilaparvata lugens (Stål) (Hemiptera:Delphacidae)

    Science.gov (United States)

    Wang, Wei-Xia; Li, Kai-Long; Chen, Yang; Lai, Feng-Xiang; Fu, Qiang

    2015-01-01

    The enolase [EC 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-PGE) to phosphoenolpyruvate (PEP). In this study, a full-length cDNA encoding α-enolase was cloned from rice brown planthopper (Nilaparvata lugens) and is provisionally designated as NlEno1. The cDNA sequence of NlEno1 was 1,851 bp with an open reading frame (ORF) of 1,305 bp and encoding 434 amino acids. The deduced protein shares high identity of 80–87% with ENO1-like protein from Hemiptera, Diptera, and Lepidoptera speices. The NlEno1 showed the highest mRNA expression level in hemolymph, followed by fat body, salivary gland, ovaries and egg, and showed trace mRNA levels in testis. The mRNA of NlEno1 showed up-regulated level in virulent N. lugens population Mudgo, IR56 and IR42 when compared with TN1 population. Injection of double-stranded RNA (dsRNA) of NlEno1 into the adults significantly down-regulated the NlEno1 mRNA level along with decreased eggs and offspring. Moreover, injection of NlEno1-dsRNA decreased mRNA level of Vitellogenin (Vg) gene. These results showed that the NlEno1, as a key glycolytic enzyme, may play roles in regulation of fecundity and adaptation of N. lugens to resistant rice varieties. PMID:26056319

  2. Recent progress in 'bioelectronics' research. Part 3. ; Study on underlying information processing mechanism of aplysia (sea slug) neural system. Baioerekutoronikusu eno michi (shorai wa saibogu mo). 3. ; Amefurashi shinkei kei no sado genri wo toki akasu (burein konpyuta) no jitsugen wo mezashite

    Energy Technology Data Exchange (ETDEWEB)

    Shiono, S. (Mitsubishi Electric Corp., Amagasaki, Hyogo (Japan). Central Research Lab.)

    1994-02-20

    Aplysia has memorizing and learning ability, and is an ideal creature for the elucidation of the learning mechanism of neural system. In addition, it has large neurons which can be studied for many purposes. Their input and output can be clearly defined, and the existence of a neural network is quite obvious which performs the processing between them. The subject which the computer in the next century must learn from living things are the principles of two neural functions, i.e. 'learning' and 'super-parallel distributed information processing.' The operating mode of the neural system is super parallel distribution which is fundamentally different from that of the serial processing computer. The method which is most worthy of notice as a means of measuring many neurons comprising the neural network simultaneously is the use of light. If optical measurement method is employed, the generation of individual action potentials of many neurons comprising the neural network can be measured independently and simultaneously. The neural system of aplysia controls the movement of the gill very well using both analog and digital neurons. 10 refs., 8 figs.

  3. Development of water purification technologies for intensive fish culture. 10. Rearing of Japanese flounder with closed seawater recirculation system equipped with immobilized bacteria; Konoritsu gyorui seisan no tame no suishitsu joka gijutsu no kaihatsu. 10. Hirame shiiku eno koteika biseibutsu no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Uemoto, H.; Watanabe, Y.; Kikuchi, K.

    1994-03-01

    Japanese flounder were reared by a seawater recirculation and filtration system with immobilized bacteria. As a water purification system, used were a nitrification tank and denitrification tank which were both filled with immobilized nitrifying bacteria and denitrifying bacteria, respectively. The flounder could be reared for 180 days with their body weight doubled at the end of experiment. Ammonia exhausted from the flounder was not accumulated in the rearing water. Nitric acid produced by the oxidation of ammonia was prevented from heightening its concentration in the denitrification tank. Usable immediately after having been filled into the filtration tank, both immobilized nitrifying and denitrifying bacteria are stable in activity without being affected by organic matter in the rearing water. The dissolution and disintegration of carrier were not confirmed. The high activity of immobilized nitrifying bacteria and denitrifying bacteria was observed on the surface and in the central part, respectively of the carrier. The activity of both bacteria was confirmed inside the carrier. From the foregoing, the high density rearing was known to be possible even in a filtration tank miniaturized to the size of about 20% of the conventional one. 29 refs., 18 figs., 2 tabs.

  4. FY 1998 result report. Study of a total system for the development of superconductor power application technology (Feasibility study of commercialization of superconductivity technology and study of the introductory effect. Future superconductivity technology development in Japan); 1998 nendo seika hokokusho. Chodendo denryoku oyo gijutsu kaihatsu total system nado no kenkyu chodendo gijutsu no jitsuyoka kanosei oyobi donyu kokanado no chosa (Nippon ni okeru chodendo gijutsu kaihatsu no kongo no hokosei)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    As a part of the New Sunshine Project, 'an R and D project on superconductor power application technology,' an examinational study was made as a mini project to clarify the developmental course for commercialization of superconductor technology. The superconductor technology is being watched with interest as a technology in the 21st century. In the application to the electric power/energy field, in particular, expected are the energy saving effect by high operation efficiency, excellent environmentality, developmental potentiality of new equipment/system by the application of ferromagnetism, etc. Accordingly, the paper analytically arranged the needs of superconductor devices in Japan and abroad and the technology seeds corresponding to the needs, and prepared the developmental subjects of superconductor technology. These developmental steps and the mutual relationship were expressed in an R and D framework. At the same time, as to the superconductivity, a survey outlined the projects carried out in each government office in Japan. The future developmental course was indicated, and proposals were made on the equipment/system as object which are the subjects for urgent development for commercialization. (NEDO)

  5. Simvastatin induces a central hypotensive effect via Ras-mediated signalling to cause eNOS up-regulation

    Science.gov (United States)

    Cheng, Wen-Han; Ho, Wen-Yu; Chang, Chien-Feng; Lu, Pei-Jung; Cheng, Pei-Wen; Yeh, Tung-Chen; Hong, Ling-Zong; Sun, Gwo-Ching; Hsiao, Michael; Tseng, Ching-Jiunn

    2013-01-01

    BACKGROUND AND PURPOSE Clinical studies indicate that statins have a BP-lowering effect in hypercholesterolemic individuals with hypertension. Specifically, statins modulate BP through the up-regulation of endothelial NOS (eNOS) activation in the brain. However, the signalling mechanisms through which statins enhance eNOS activation remain unclear. Therefore, we examined the possible signalling pathways involved in statin-mediated BP regulation in the nucleus tractus solitarii (NTS). EXPERIMENTAL APPROACH To investigate the involvement of Ras and other signalling pathways in simvastatin-induced effects on BP, BP and renal sympathetic nerve activity (RSNA) were determined in spontaneously hypertensive rats (SHRs) before and after i.c.v. administration of simvastatin in the absence and presence of a Ras-specific inhibitor (farnesyl thiosalicylic acid, FTS), a geranylgeranyltransferase inhibitor (GGTI-2133), a PI3K inhibitor (LY294002) or a MAPK-ERK kinase (MEK) inhibitor (PD98059). KEY RESULTS FTS significantly attenuated the decrease in BP and increased NO evoked by simvastatin and reversed the decrease in basal RSNA induced by simvastatin. Immunoblotting and pharmacological studies showed that inhibition of Ras activity by FTS significantly abolished simvastatin-induced phosphorylation of ERK1/2, ribosomal protein S6 kinase (RSK), Akt and decreased eNOS phosphorylation. Likewise, administration of Akt and ERK1/2 signalling inhibitors, LY294002 and PD98059, attenuated the reduction in BP evoked by simvastatin. Furthermore, i.c.v. simvastatin decreased Rac1 activation and the number of ROS-positive cells in the NTS. CONCLUSIONS AND IMPLICATIONS Simvastatin modulates central BP control in the NTS of SHRs by increasing Ras-mediated activation of the PI3K-Akt and ERK1/2-RSK signalling pathways, which then up-regulates eNOS activation. PMID:23889671

  6. FEATURES OF THE CLINICAL SIGNIFICANCE OF POLYMORPHIC VARIANTS OF ENOS AND AGTR2 GENES IN PATIENTS WITH CAD

    Directory of Open Access Journals (Sweden)

    A. L. Khokhlov

    2016-01-01

    Full Text Available Coronary heart disease (CHD is a major cause of mortality. Morphological substrate of CHD in most cases is atherosclerosis, which is based on structural genes polymorphism eNOS and AGTR2. The aim of the study was to study the prevalence of eNOS and AGTR2 genes in patients with coronary artery disease and the association of these genes with coronary heart disease. The study involved 187 patients aged 36 to 86 years (62,2±11,2 with different forms of CHD: stable and unstable angina, myocardial infarction and 45 people without CHD. Determination of gene polymorphisms was performed by real-time PCR analyzer of nucleic acids IQ 5 Bio-Rad. Statistical analysis was performed using Statistica 10.0. The study revealed a significant difference between the incidence of homozygous AA allelic variant gene AGTR2 group of patients with myocardial infarction and the comparison group; polymorphic variant AA AGTR2 gene is associated with earlier onset of coronary artery disease; It found that carriers of the polymorphic variant gene GA AGTR2 beginning statistically CHD occurred significantly later than in carriers of alleles GG and AA; age CHD debut TT allele carriers of the eNOS gene is associated with an earlier onset of the disease and statistically significantly different from the age of first CHD in carriers of alleles of polymorphic variants of GG and GT; revealed a positive correlation between the polymorphic allele AGTR2 gene with the presence of arterial hypertension in patients with coronary artery disease; It determined that the T allele carriers of the polymorphic gene eNOS is associated more early onset of hypertension, found the association of the polymorphic allele gene AGTR2 the need to use higher doses of ACE inhibitor — perindopril.

  7. Metformin stimulates ischemia-induced revascularization through an eNOS dependent pathway in the ischemic hindlimb mice model.

    Science.gov (United States)

    Takahashi, Noriko; Shibata, Rei; Ouchi, Noriyuki; Sugimoto, Masayuki; Murohara, Toyoaki; Komori, Kimihiro

    2015-02-01

    As first-line treatment for type 2 diabetes, metformin has gained a strong position. In addition, type 2 diabetics benefit from the fact that metformin is associated with a reduction in cardiovascular events. Nevertheless, there is a dearth of information concerning the functional role of metformin in regulating angiogenesis. Our present study explores whether metformin is involved in the modulation of the revascularization processes in vivo by employing a hindlimb mice model of ischemia-induced angiogenesis. For comparative purposes, randomly selected wild-type (WT) mice or endothelial nitric oxide synthase (eNOS) deficient mice were assigned to one of two groups. One group was orally administered a daily dose of metformin through a gastric tube whereas the other group served as a control with no metformin administered. Both groups were subjected to unilateral hindlimb ischemia. Laser Doppler analysis coupled with capillary density staining with CD31was the method employed to determine revascularization. Adenosine monophosphate-activated protein kinase (AMPK) and eNOS phosphorylation levels were assessed using Western blot analysis. Subsequent to hindlimb ischemic surgery, in comparison to the nontreated mice, metformin-treated WT mice showed accelerated limb perfusion, which was substantiated by laser Doppler blood-flow measurements and the presence of increased capillary density in the ischemic adductor muscle. Treatment with metformin significantly enhanced the increase in AMPK and eNOS phosphorylation levels of muscle tissues in WT mice induced by ischemia. In eNOS- deficient knockout mice, there was a significant increase in ischemic tissue AMPK phosphorylation induced by metformin; however, blood flow recovery in ischemic limb after surgery was unaffected. Metformin promoted revascularization in the presence of tissue ischemia through an AMPK/eNOS-related mechanism. Our study indicates that, in addition to its glucose-lowering effect, metformin fosters

  8. Immunomodulatory effects of royal jelly on aorta CD3, CD68 and eNOS expression in hypercholesterolaemic rats

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    Amal Attia El-Morsy Ibrahim

    2014-08-01

    RJ administration (300 mg/kg/day with CH, produced a counteractive effect, represented in recompense of biochemical alteration, CD4 and CD8 expression. RJ intake also amended the histological picture. The immunohistochemical picture revealed a decrease in CD3, CD86 and eNOS in the aortic tissue. These findings attributed to the significant immunomodulatory effect of RJ remedy suppress deleterious effects of hypercholesterolaemia.

  9. Malaria knowledge and agricultural practices that promote mosquito breeding in two rural farming communities in Oyo State, Nigeria

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    Oshiname Frederick O

    2010-04-01

    Full Text Available Abstract Background Agricultural practices such as the use of irrigation during rice cultivation, the use of ponds for fish farming and the storage of water in tanks for livestock provide suitable breeding grounds for anthropophylic mosquitoes. The most common anthropophylic mosquito in Nigeria which causes much of the morbidity and mortality associated with malaria is the anopheles mosquito. Farmers are therefore at high risk of malaria - a disease which seriously impacts on agricultural productivity. Unfortunately information relating to agricultural practices and farmers' behavioural antecedent factors that could assist malaria programmers plan and implement interventions to reduce risk of infections among farmers is scanty. Farmers' knowledge about malaria and agricultural practices which favour the breeding of mosquitoes in Fashola and Soku, two rural farming communities in Oyo State were therefore assessed in two rural farming communities in Oyo State. Methods This descriptive cross-sectional study involved the collection of data through the use of eight Focus Group Discussions (FGDs and the interview of 403 randomly selected farmers using semi-structured questionnaires. These sets of information were supplemented with observations of agricultural practices made in 40 randomly selected farms. The FGD data were recorded on audio-tapes, transcribed and subjected to content analysis while the quantitative data were analyzed using descriptive and inferential statistics. Results Most respondents in the two communities had low level of knowledge of malaria causation as only 12.4% stated that mosquito bite could transmit the disease. Less than half (46.7% correctly mentioned the signs and symptoms of malaria as high body temperature, body pains, headache, body weakness and cold/fever. The reported main methods for preventing mosquito bites in the farming communities included removal of heaps of cassava tuber peelings (62.3%, bush burning

  10. Efectos ecológicos del fenómeno ENOS en Colombia

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    Yesid Carvajal

    2014-06-01

    Full Text Available El fenómeno ENOS, tiene un sustancial efecto sobre las variaciones del clima a nivel mundial; por lo tanto, no sorprende que sus efectos tengan un impacto ecológico y socioeconómico fuerte, alterando la seguridad alimentaria de los países a nivel mundial. Ante este panorama, es comprensible que haya despertado el mayor interés mundial, no sólo en los especialistas en meteorología y oceanografía sino también entre biólogos, ecólogos, físicos, matemáticos, agrónomos, hidrólogos, economistas, sociólogos y planificadores, entre otros. En el presente documento, se muestra algunos de los principales efectos ecológicos en Colombia durante las fases extremas cálida (El Niño y fría (La Niña, indicando que es una necesidad urgente, orientar la investigación en sus causas, consecuencias e impactos.

  11. Targeted ENO schemes with tailored resolution property for hyperbolic conservation laws

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    Fu, Lin; Hu, Xiangyu Y.; Adams, Nikolaus A.

    2017-11-01

    In this paper, we extend the range of targeted ENO (TENO) schemes (Fu et al. (2016) [18]) by proposing an eighth-order TENO8 scheme. A general formulation to construct the high-order undivided difference τK within the weighting strategy is proposed. With the underlying scale-separation strategy, sixth-order accuracy for τK in the smooth solution regions is designed for good performance and robustness. Furthermore, a unified framework to optimize independently the dispersion and dissipation properties of high-order finite-difference schemes is proposed. The new framework enables tailoring of dispersion and dissipation as function of wavenumber. The optimal linear scheme has minimum dispersion error and a dissipation error that satisfies a dispersion-dissipation relation. Employing the optimal linear scheme, a sixth-order TENO8-opt scheme is constructed. A set of benchmark cases involving strong discontinuities and broadband fluctuations is computed to demonstrate the high-resolution properties of the new schemes.

  12. A System Analysis of the Demand for Animal Protein in Rural and ...

    African Journals Online (AJOL)

    A System Analysis of the Demand for Animal Protein in Rural and Urban Nigeria: A Case Study of Ibadan Metropolis. ... The cross price elasticity also showed that beef and chicken are luxury goods in the study area and fish is a necessity good. Keywords: Demand, Protein consumption, rural and urban households, Oyo ...

  13. Determination of Location and Depth of Mineral Rocks at Olode Village in Ibadan, Oyo State, Nigeria, Using Geophysical Methods

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    Akintayo O. Ojo

    2014-01-01

    Full Text Available Magnetic and resistivity geophysical methods were used to investigate the location and depth of mineral rocks at Olode village, Oyo State, Nigeria. 80 magnetic data points were acquired in 10 profiles using G-816 proton precession magnetometer with 10 m spacing in between each profiles and 10 m stations interval. After correcting diurnal variations, the raw magnetic data obtained were plotted as 2D and 3D magnetic contour maps. The residual anomalies obtained were plotted against distance using Microsoft Excel, and Peter’s half-slope method was used to find the depth to the magnetic sources. The magnetic signature obtained show considerable varying amplitude from a minimum value of 155.3 nT at a depth of 6.37 m to a maximum value of 670.3 nT at a depth of 6.25 m. Resistivity data were obtained using Campus Tiger resistivity meter and 9 VESs were acquired using Schlumberger configuration. All VESs were 150 m long, 75 m on each side of the referenced points which were 10 m apart. VESs 1–4 and 7–9 are underlined with rocks of high conductivity and susceptibility values. VESs 5 and 6 show rocks with low conductivity and susceptibility values. The results of these geophysical methods show that there are rocks with high magnetic susceptibility and conductivity values from the centre towards the eastern region of the study area and low magnetic susceptibility and conductivity values at the western region. On the average, the depths of these rocks from the surface fall in the interval of 5.80 m to 6.72 m.

  14. Contribution of household health care expenditure to poverty in Oyo State, South West Nigeria: A rural and urban comparison

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    Olayinka Stephen Ilesanmi

    2017-07-01

    Full Text Available Introduction: The financial burden of health care costs in Nigeria is borne almost entirely by the individuals and household members as health care financing is still mostly from out of pocket (OOP payments. OOP payments can lead households into poverty. This study aimed to estimate the contribution of household health care expenditure to poverty in rural and urban communities in Oyo state, Nigeria. Method: This is a comparative cross-sectional study using a tested and adapted version of the Living Standard Survey questionnaire to collect data on 5,696 household members from 1,434 household representatives. Representatives were selected using a multistage sampling method. Information was collected from 714(49.8% and 720(50.2% households in the urban and rural Local Government Area (LGA, respectively. International poverty line of $1.25 per day was used. Poverty level was measured with and without household health expenditure. An exact McNemar’s test was used to determine the difference in the proportion of poor, gross and net payment for health care services. SPSS software was used for data analysis. Results: Health care was utilised by 1,006 (70.2% of the 1,434 households studied. Of urban and rural households, 637(89.2% and 369(51.3% utilized health care services, respectively. Only 513(29.8% were poor while 1519(88.2% were poor after considering the cost of utilising health care. Increase in poverty of 66.2% occurred because of health care utilisation (p<0.001. Conclusion: Health care expenditure increased the proportion of household members living below poverty line. To protect against poverty free basic health care services is required in Nigeria.

  15. Dental health service utilisation by resident doctors/medical officers in the University College Hospital, Ibadan, Oyo State, Nigeria.

    Science.gov (United States)

    Opeodu, O I; Dosumu, E B; Arowojolu, M O

    2012-09-01

    Dental disease is still a serious health problem universally possibly because dental health awareness and dental service utilization is still very low. The reported prevalence of dental diseases, especially periodontal disease, is higher among the black race while their rate of dental service utilization is lower in comparison to Caucasians. The aim of this study was to assess the rate of dental service utilization and identify the factors influencing such utilization by medical doctors and dentists in the University College Hospital, Ibadan. Two hundred 26-item questionnaires were distributed among the resident doctors/medical officers in the University College Hospital, Ibadan, Oyo state. The questionnaire sought to know if there is any previous dental visits, the reason for such visits and any other follow-up visits afterward. One hundred and ninety of the doctors returned the filled questionnaires giving a response rate of 95%. Data analysis was done using Statistical Package for Social Sciences (SPSS) version 14.0. Level of statistical significance was set at p dental visit but only 25.3% was within the last one year of the study. Among those that had visited a dentist before, 59.7% did so for the symptomatic treatment of one ailment or the other and only 13.7% of them went back for the follow-up appointment after the resolution of their symptoms. There was a statistically significant difference between the rate of dental service utilization and the gender of the respondents (p dental service utilization and age of the respondents. The study showed that the rate of dental service utilization is low when compared with that of developed countries. Some factors identified as contributing to the lack of dental service utilization among the participants include prolonged and repeated dental appointments and the demand of their work.

  16. Prevalence and correlates of the perpetration of cyberbullying among in-school adolescents in Oyo State, Nigeria.

    Science.gov (United States)

    Olumide, Adesola O; Adams, Patricia; Amodu, Olukemi K

    2016-05-01

    Cyberharassment/cyberbullying is a global problem that has been inadequately investigated in developing countries. In this paper, we present findings on the prevalence and predictors of perpetration of cyberbullying among in-school adolescents in Oyo state, Nigeria. A total of 653 students were selected via multi-stage sampling. Information on history of perpetrating harassment via an electronic medium in the 3-month period preceding the survey was obtained. Respondents' mean age was 14.2±2.2 years and 51.3% were females. All respondents had personal mobile phones and about half had Internet access. About 40% accessed the Internet every day while about 48% accessed it at least once to several times a week and <5% accessed it about once every 2 weeks. One hundred and fifty-six (23.9%) had harassed someone electronically, 260 (39.8%) had been victimized, and 137 (21.0%) were both victims and perpetrators. Common modes of harassment were via phone calls 99 (63.5%), chat rooms 70 (44.9%), and text messages 60 (38.5%). Students who had been victims of cyberbullying (OR=21.76, 95% CI=12.64-37.47) and those with daily Internet access (OR=2.32, 95% CI=1.28-4.19) had significantly higher Oods of being perpetrators. About a quarter of students were perpetrators of cyberbullying, and the correlates of perpetration were history of cyber victimization and daily Internet access. Intervention programs must be instituted for victims as well as frequent users of the Internet to curb the problem in the study area.

  17. Activities and influence of veterinary drug marketers on antimicrobial usage in livestock production in Oyo and Kaduna States, Nigeria

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    Olufemi Ernest Ojo

    2017-09-01

    Full Text Available Antimicrobial usage in animals contributes to the emergence of antimicrobial resistant bacterial strains. Investigations were carried out on how the characteristics, knowledge, attitude and practices of antimicrobial marketers influenced antimicrobials usage in animal production in Oyo and Kaduna States, Nigeria. Focus group discussions, in-depth interviews and structured questionnaires were used to gather information about the characteristics and activities of antimicrobial marketers. Overall, 70 (56.9 % of 123 marketers had post-secondary education while 76 (61.8 % were trained on the use of antimicrobials. Eighteen (14.6 % of the marketers were licensed veterinarians. Only 51 (41.5 % marketers displayed adequate knowledge about antimicrobials and antimicrobial usage. Sixty-seven (54.6 % marketers requested a prescription before selling antimicrobials while 113 (91.9 % marketer recommended antimicrobials for use in animals. Two-third of the marketers (66.7 % prescribed antimicrobials without physically examining sick animals but based their prescriptions on verbal reports of clinical signs by farmers and on their personal experience. Marketers with higher educational qualification displayed more adequate knowledge of antimicrobials and antimicrobial usage than those with basic education background only. More years of experience in antimicrobial marketing did not translate to better knowledge on antimicrobial usage. Only 45 (36.6 % respondents were aware of the existence of regulatory agencies monitoring the use of antimicrobials in animals. Farmers ignored the services of veterinarians in the diagnosis and control of animal diseases but resorted to drug marketers for help. Effective communication of existing legislations on antimicrobial usage, improved access to veterinary services and strict enforcement of regulatory policies are recommended for checking non-judicious use of antimicrobial agents in animal production. Sales of

  18. Effect of irrigation on soil health: a case study of the Ikere irrigation project in Oyo State, southwest Nigeria.

    Science.gov (United States)

    Adejumobi, M A; Awe, G O; Abegunrin, T P; Oyetunji, O M; Kareem, T S

    2016-12-01

    Irrigated agriculture is one of the significant contributors to the food security of the millennium development goals (MDGs); however, the modification of soil matrix by irrigation could alter the overall soil health due to changes in soil properties and processes. The objective of the study was to evaluate the effect of irrigation on soil quality status of the Ikere center pivot irrigation project site in Oyo State, southwest Nigeria. Disturbed soil samples were collected from 0 to 30, 30 to 60, and 60 to 90-cm layers from four different sites in three replicates, within the project location for the determination of soil bio-chemical properties. The average values of sodium adsorption ratio (SAR) soil condition is normal in relation to salinity and sodicity hazards. The effective cation exchange capacity (ECEC), soil organic matter (SOM), total nitrogen (TN), and calcium ion (Ca(2+)) concentrations were low while the available phosphorus (P) was moderate. The principal component analysis showed EC, ECEC, SAR, SOM, and TN as the minimum data set (MDS) for monitoring and assessing the soil quality status of this irrigation field. In terms of bio-chemical properties, the soil quality index (SQI) of the field was average (about 0.543) while the sampling locations were ranked as site 2 > site 4 > site 3 > site 1 in terms of SQI. The results of this study are designated as baseline for future evaluation of soil quality status of this irrigation field and further studies should incorporate soil physical and more biological properties when considering overall soil quality status.

  19. Up-Regulation of ENO1 by HIF-1α in Retinal Pigment Epithelial Cells after Hypoxic Challenge Is Not Involved in the Regulation of VEGF Secretion.

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    Feihui Zheng

    Full Text Available Alpha-enolase (ENO1, a major glycolytic enzyme, is reported to be over-expressed in various cancer tissues. It has been demonstrated to be regulated by the Hypoxia-inducible factor 1-α (HIF-1α, a crucial transcriptional factor implicated in tumor progression and cancer angiogenesis. Choroidal neovascularization (CNV, which is a leading cause of severe vision loss caused by newly formed blood vessels in the choroid, is also engendered by hypoxic stress. In this report, we investigated the expression of ENO1 and the effects of its down-regulation upon cobalt (II chloride-induced hypoxia in retinal pigment epithelial cells, identified as the primary source of ocular angiogenic factors.HIF-1α-diminished retinal pigment epithelial cells were generated by small interfering RNA (siRNA technology in ARPE-19 cells, a human retinal pigment epithelial cell line. Both normal and HIF-1α-diminished ARPE-19 cells were then subjected to hypoxic challenge using cobalt (II chloride (CoCl2 or anaerobic chamber. The relation between ENO1 expression and vascular endothelial growth factor (VEGF secretion by retinal pigment epithelial cells were examined. Protein levels of HIF-1α and ENO1 were analyzed using Western Blot, while VEGF secretion was essayed by enzyme-linked immunosorbent assay (ELISA. Cytotoxicity after hypoxia was detected by Lactate Dehydrogenase (LDH Assay.Upon 24 hr of CoCl2-induced hypoxia, the expression levels of ENO1 and VEGF were increased along with HIF-1α in ARPE-19 cells, both of which can in turn be down-regulated by HIF-1α siRNA application. However, knockdown of ENO1 alone or together with HIF-1α did not help suppress VEGF secretion in hypoxic ARPE-19 cells.ENO1 was demonstrated to be up-regulated by HIF-1α in retinal pigment epithelial cells in response to hypoxia, without influencing VEGF secretion.

  20. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus.

    Science.gov (United States)

    Youn, Ji-Youn; Zhou, Jun; Cai, Hua

    2015-08-01

    We have recently shown that angiotensin II-mediated uncoupling of endothelial nitric oxide synthase (eNOS) contributes to endothelial dysfunction in streptozotocin-induced type 1 diabetes mellitus. However, it has remained unclear whether and how eNOS uncoupling occurs in type 2 diabetes mellitus (T2DM) and the consequences of such in regulating vascular function. Here we investigated a role of bone morphogenic protein (BMP)-4 in mediating eNOS uncoupling, endothelial dysfunction, and inflammation in db/db mice. Circulating levels of BMP4 were markedly elevated in db/db mice but not in mice with type 1 diabetes mellitus, in which angiotensin II levels were significantly increased. Infusion of BMP4 antagonist noggin into db/db mice (15 μg/kg/day, 4 weeks) abolished eNOS uncoupling activity while restoring tetrahydrobiopterin (H(4)B) bioavailability. The impaired endothelium-dependent vasorelaxation in db/db aortas was significantly improved by noggin infusion. Exposure of aortic endothelial cells to BMP4 (50 ng/mL, 24 hours) resulted in eNOS uncoupling, which was attenuated by H(4)B precursor sepiapterin or small interfering RNA silencing nicotinamide adenine dinucleotide phosphate oxidase isoform 1 (NOX1). Interestingly, BMP4-dependent NOX1 up-regulation was abrogated by sepiapterin, implicating a NOX1-uncoupled eNOS-NOX1 feed-forward loop. BMP4 induction of cyclooxygenase 2 (COX2) expression and vascular cell adhesion protein 1 was found in db/db mice. Consistently, COX2 was up-regulated by BMP4 in endothelial cells, which was attenuated by sepiapterin, implicating an upstream role of eNOS uncoupling in COX2-mediated inflammatory activation. Taken together, our data for the first time reveal a novel role of BMP4 in inducing NOX1-dependent eNOS uncoupling in T2DM, which may promote development of novel therapeutics restoring endothelial function in T2DM.

  1. Polymorphism of the gene eNOS G894T (Glu298Asp) in symptomatic patients with aterosclerosis.

    Science.gov (United States)

    Campedelli, F L; E Silva, K S F; Rodrigues, D A; Martins, J V M; Costa, I R; Lagares, M H; Barbosa, A M; de Morais, M P; Moura, K K V O

    2017-05-04

    Atherosclerotic and its cardiovascular complications are responsible for 17.5 million deaths a year, according to the World Health Organization. There is consensus that atherosclerosis involves multiple pathogenic processes initiated by endothelial dysfunction, with inflammation and vascular proliferation determining alterations in the matrix, with consequent formation of the atheromatous plaque and its clinical implications. Risk factors such as hypertension, diabetes mellitus, dyslipidemia, and smoking are widely known. Currently, genotyping, which is not directly related to these factors, is not accepted to estimate the risk of cardiovascular diseases, but strong evidence indicates several polymorphic genes as factors of risk and progression leading to complications of the disease. Among the genes involved, eNOS (endothelial nitric oxide synthase gene), which is responsible for the production of endothelial nitric oxide (an important arterial vasodilator), when presented in polymorphic variation can determine production, malfunction, and predisposition to atherosclerosis. In the present study, we analyzed the G894T polymorphism of the eNOS gene in groups of individuals diagnosed with atherosclerosis and in a control group. We collected 200 blood samples from patients previously diagnosed with atherosclerosis and 100 samples formed the control group. The genotyping analysis for polymorphism of the eNOS gene was determined by PCR. We considered variables such as gender, smoking, smoking history, and alcohol consumption; statistical differences were found in the distribution of case and control groups (P = 0.0378) and in non-smoking patients (P = 0.0263). In the other associations, no statistically significant difference was found. In the population studied, the frequency of the heterozygous genotype (GT) was much higher than in the other populations (GG and TT) in both groups (case and control). The GG genotype showed greater susceptibility to atherosclerosis

  2. Konsumsi Antioksidan dan Ekspresi Gen eNOS3 Alel -786T>C pada Penderita Hipertensi Etnik Minangkabau

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    Delmi Sulastri

    2011-03-01

    Full Text Available Gene of eNOS 3 allel -786T>C is one of the important genes which is related to the high prevalence of hypertension. Polymorphism of this gene decreases nitric oxide synthase (NOS enzyme synthesis result in reduction of nitric oxide (NO. Nitric oxide causes vasodilatation, which decreases peripheral resistant and blood pressure. The aim of this study was to evaluate the influence of an antioxidant consumption on eNOS3 gene -786T>C allel expression in hypertension patients with Minangkabau ethnicity. Cross sectional study was done on 130 hypertension and normotension subjects, 30–65 years old in 4 districts, Padang, Sumatera Barat during 2009. The interview about antioxidant consumption by food frequency questioner, the assessment of eNOS3 gene by polymerase chain reaction and NO plasma level by enzyme linked immunosorbent assay were done. The data were analyzed using t-test and chi-square. The results showed that the hypertensive’s and normotensive’s plasma NO concentration were 26.91±15.40 μM/L and 25.79±15.04 μM/L, respectively. There were thymine to cytosine substitution found at 786 position in 1.5% of the hypertensive subjects and 9.2% of the normotensive subjects. A low plasma NO levels (67.2% was found in subjects with heterozygote TC alleles.There was a significant relationship between consumption of antioxidant (vitamin E and NO plasma level in hypertension subjects with TC heterozygote allel. In conclusions, vitamin E influences NO plasma level in hypertension patients with heterozygote TC alleles.

  3. eNOS gene Glu298Asp and 4b/a polymorphisms are associated with renal function parameters in Mexican patients with Fabry disease.

    Science.gov (United States)

    Marin-Medina, A; Brambila-Tapia, A J L; Picos-Cárdenas, V J; Gallegos-Arreola, M P; Figuera, L E

    2016-10-24

    Fabry disease (FD) is an inherited X-linked lysosomal disease that causes renal failure in a high percentage of affected individuals. The eNOS gene encodes for endothelial nitric oxide synthase, which plays an important role in glomerular hemodynamics. This gene has two main polymorphisms (Glu298Asp and 4b/a) that have been studied in the context of many different diseases, including those involving cardiovascular and renal alterations. Considering the lack of information regarding eNOS variants and FD, we investigated whether there were associations between eNOS genetic variants and renal function parameters in Mexican patients with FD and renal impairment. In total, 15 FD patients with renal alterations were included in the present study, and associations between eNOS polymorphisms and renal function parameters (urea, creatinine, and GFR) were evaluated. The Asp298 and 4a alleles of the eNOS gene were found to be significantly associated with increased levels of urea and creatinine, and a decreased glomerular filtration rate in FD patients, and this association behaved in a co-dominant fashion. Our results coincide with previous reports showing an association between these polymorphisms and kidney disease, and along with other studies regarding their role in the nitric oxide pathway, suggest that these variants affect the severity of nephropathy in patients with FD.

  4. Low-energy laser irradiation increases endothelial cell proliferation, migration, and eNOS gene expression possibly via PI3K signal pathway.

    Science.gov (United States)

    Chen, Chung-Huang; Hung, Huey-Shan; Hsu, Shan-Hui

    2008-01-01

    The purpose of this study, therefore, was to determine the mechanisms by which low-energy laser irradiation (LELI) may exert some of its angiogenic effects via the PI3 kinase/eNOS signaling pathway and induce endothelial cell migration and neovascularization, an important and necessary part of wound healing. The possible molecular mechanism of helium-neon (He-Ne) laser irradiation on endothelial cells was proposed. He-Ne laser at 632.5 nm was used to stimulate human umbilical vein endothelial cell (HUVEC), and its effect on cell proliferation, nitric oxide secretion, and cell migration was determined. Irradiation enhanced endothelial nitric oxidase synthase (eNOS) protein expression, and irradiation of less than 0.26 J/cm(2) enhanced eNOS gene expression in HUVEC. The cell migration ability was promoted for HUVEC irradiated with 0.26 J/cm(2). This agreed with the vinculin protein expression induced by irradiation. In addition, the angiogenesis was promoted. The induced eNOS expression was inhibited by LY294002, indicating that the effect of laser on EC could be attributed to the up-regulation of eNOS expression through PI3K pathway at the cellular and molecular levels as a result of the He-Ne laser. The study has shown that LELI increased endothelial cell proliferation, migration, NO secretion, and identified that activation of PI3K/Akt pathway was a critical step for the elevated for eNOS expression upon LELI.

  5. Synergistic Antihypertensive Effect of Carthamus tinctorius L. Extract and Captopril in l-NAME-Induced Hypertensive Rats via Restoration of eNOS and AT1R Expression

    Science.gov (United States)

    Maneesai, Putcharawipa; Prasarttong, Patoomporn; Bunbupha, Sarawoot; Kukongviriyapan, Upa; Kukongviriyapan, Veerapol; Tangsucharit, Panot; Prachaney, Parichat; Pakdeechote, Poungrat

    2016-01-01

    This study examined the effect of Carthamus tinctorius (CT) extract plus captopril treatment on blood pressure, vascular function, nitric oxide (NO) bioavailability, oxidative stress and renin-angiotensin system (RAS) in Nω-Nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Rats were treated with l-NAME (40 mg/kg/day) for five weeks and given CT extract (75 or 150 or 300 or 500 mg/kg/day): captopril (5 mg/kg/day) or CT extract (300 mg/kg/day) plus captopril (5 mg/kg/day) for two consecutive weeks. CT extract reduced blood pressure dose-dependently, and the most effective dose was 300 mg/kg/day. l-NAME-induced hypertensive rats showed abnormalities including high blood pressure, high vascular resistance, impairment of acetylcholine-induced vasorelaxation in isolated aortic rings and mesenteric vascular beds, increased vascular superoxide production and plasma malondialdehyde levels, downregulation of eNOS, low level of plasma nitric oxide metabolites, upregulation of angiotensin II type 1 receptor and increased plasma angiotensin II. These abnormalities were alleviated by treatment with either CT extract or captopril. Combination treatment of CT extract and captopril normalized all the abnormalities found in hypertensive rats except endothelial dysfunction. These data indicate that there are synergistic antihypertensive effects of CT extract and captopril. These effects are likely mediated by their anti-oxidative properties and their inhibition of RAS. PMID:26938552

  6. Synergistic Antihypertensive Effect of Carthamus tinctorius L. Extract and Captopril in l-NAME-Induced Hypertensive Rats via Restoration of eNOS and AT1R Expression

    Directory of Open Access Journals (Sweden)

    Putcharawipa Maneesai

    2016-02-01

    Full Text Available This study examined the effect of Carthamus tinctorius (CT extract plus captopril treatment on blood pressure, vascular function, nitric oxide (NO bioavailability, oxidative stress and renin-angiotensin system (RAS in Nω-Nitro-l-arginine methyl ester (l-NAME-induced hypertension. Rats were treated with l-NAME (40 mg/kg/day for five weeks and given CT extract (75 or 150 or 300 or 500 mg/kg/day: captopril (5 mg/kg/day or CT extract (300 mg/kg/day plus captopril (5 mg/kg/day for two consecutive weeks. CT extract reduced blood pressure dose-dependently, and the most effective dose was 300 mg/kg/day. l-NAME-induced hypertensive rats showed abnormalities including high blood pressure, high vascular resistance, impairment of acetylcholine-induced vasorelaxation in isolated aortic rings and mesenteric vascular beds, increased vascular superoxide production and plasma malondialdehyde levels, downregulation of eNOS, low level of plasma nitric oxide metabolites, upregulation of angiotensin II type 1 receptor and increased plasma angiotensin II. These abnormalities were alleviated by treatment with either CT extract or captopril. Combination treatment of CT extract and captopril normalized all the abnormalities found in hypertensive rats except endothelial dysfunction. These data indicate that there are synergistic antihypertensive effects of CT extract and captopril. These effects are likely mediated by their anti-oxidative properties and their inhibition of RAS.

  7. Mechanotransduction in small intestinal submucosa scaffolds: fabrication parameters potentially modulate the shear-induced expression of PECAM-1 and eNOS.

    Science.gov (United States)

    Sánchez-Palencia, Diana; Rathan, Swetha; Ankeny, Casey J; Fogg, Ruth; Briceño, Juan C; Yoganathan, Ajit P

    2017-05-01

    In small intestinal submucosa (SIS) scaffolds for functional tissue engineering, the impact of scaffold fabrication parameters on cellular response and tissue regeneration may relate to the mechanotransductory properties of the final arrangement of collagen fibres. We previously proved that two fabrication parameters, (a) preservation (P) or removal (R) of a dense collagen layer present in SIS, and (b) SIS in a final dehydrated (D) or hydrated (H) state, have an effect on the micromechanical environment of SIS. In a continuation of our studies, we herein hypothesized that these fabrication parameters also modulate early mechanotransduction in cells populating the scaffold. Mechanotransduction was investigated by seeding human umbilical vein endothelial cells (HUVECs) on scaffolds, exposing them to pulsatile shear stress (12 ± 4 dyne/cm(2) ) for 1 h (n = 5) in a cone-and-plate shear system, and evaluating the expression of the mechanosensitive genes Pecam1 and Enos by immunofluorescence and qPCR. Expression of mechanosensitive genes was highest in PD grafts, followed by PH and RH grafts. The RD group had similar expression to that of unsheared control cells, suggesting that the RD combination potentially reduced mechanotransduction of shear to cells. We concluded that the two fabrication parameters studied, which modify SIS micromechanics, also potentially modulated the early shear-induced expression of mechanosensitive genes in seeded HUVECs. Our findings suggest that fabrication parameters influence the outcome of SIS as a therapeutic scaffold. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  8. Application of liquid atomization to the pharmaceutical process. Iyaku ni okeru biryuka no oyo gijutsu

    Energy Technology Data Exchange (ETDEWEB)

    Takei, N. (Freund Industrial Co. Ltd., Saitama (Japan))

    1991-04-20

    A coating system is introduced as a liquid atomization technique for oral administration drug applied to the pharmaceutical process. Controlled release of drug dissolution in the human body is important. How to dissolve the drug by coating granules is described here. For example, the core of a drug granule is made up by spherical granulated sucrose, the intermediate layer as a main element by theophylline which is an asthma medicine, the outer layer by an ethylcellulose film. Theophylline takes 30 minutes to dissolve and the ethylcellulose-coated granule does more than 16 hours. A fluidized tumbled bed type coating apparatus is developed to prevent unevenness, adhesion and aggregation. Even in the same chemical species, the dissolution speed differs in a crystal type, crystalline or amorphous and anhydrous or hydrate. Acceleration of the drug dissolution is studied. A suspension which is an ethanol solution of hydroxy propy/cellulose with supersaturated antiflammatory drug, atomized by a jetcoating system, is coated on the surface of potato starch powder. The drug as a main element shows only a 10% dissolution rate in 8 hours. On the other hand, the drug coated show a 100% dissolution rate in 2 hours. This is because the coating crystallizes the drug itself in the film and promotes its dissolution. 6 refs., 8 figs.

  9. Rural-urban disparity in knowledge and compliance with traffic signs among young commercial motorcyclists in selected local government areas in Oyo State, Nigeria.

    Science.gov (United States)

    Olumide, Adesola O; Owoaje, Eme T

    2017-06-01

    This study compared knowledge and compliance with traffic signs among young commercial motorcyclists in rural and urban communities in Oyo state, Nigeria. Information on knowledge and compliance with 10 common traffic signs was obtained from 149 rural and 113 urban commercial motorcyclists aged 18-35 years. Aggregate knowledge scores were computed and categorized as good (≥5) and poor (signs (p signs (OR = 58.15; 95% CI = 11.96-282.79). A higher proportion of rural than urban motorcyclists never obeyed any of the traffic signs. Young rural commercial motorcyclists' knowledge and compliance with the road signs was poorer than their urban counterparts. Interventions to improve the rural motorcyclists' knowledge and ultimately compliance with road signs are urgently required.

  10. Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation

    Directory of Open Access Journals (Sweden)

    Ho Sub Lee

    2012-01-01

    Full Text Available Vascular inflammation is an important factor which can promote diabetic complications. In this study, the inhibitory effects of aqueous extract from Prunella vulgaris (APV on high glucose (HG-induced expression of cell adhesion molecules in human umbilical vein endothelial cells (HUVEC are reported. APV decreased HG-induced expression of intercellular adhesion molecule-1 (ICAM-1, vascular cell adhesion molecule-1 (VCAM-1, and E-selectin. APV also dose-dependently inhibited HG-induced adhesion of HL-60 monocytic cells. APV suppressed p65 NF-κB activation in HG-treated cells. APV significantly inhibited the formation of intracellular reactive oxygen species (ROS. HG-stimulated HUVEC secreted gelatinases, however, APV inhibited it. APV induced Akt phosphorylation as well as activation of heme oxygenase-1 (HO-1, eNOS, and nuclear factor E2-related factor 2 (Nrf2, which may protect vascular inflammation caused by HG. In conclusion, APV exerts anti-inflammatory effect via inhibition of ROS/NF-κB pathway by inducing HO-1 and eNOS expression mediated by Nrf2, thereby suggesting that Prunella vulgaris may be a possible therapeutic approach to the inhibition of diabetic vascular diseases.

  11. New Findings in eNOS gene and Thalidomide Embryopathy Suggest pre-transcriptional effect variants as susceptibility factors.

    Science.gov (United States)

    Kowalski, Thayne Woycinck; Fraga, Lucas Rosa; Tovo-Rodrigues, Luciana; Sanseverino, Maria Teresa Vieira; Hutz, Mara Helena; Schuler-Faccini, Lavínia; Vianna, Fernanda Sales Luiz

    2016-03-23

    Antiangiogenic properties of thalidomide have created an interest in the use of the drug in treatment of cancer. However, thalidomide is responsible for thalidomide embryopathy (TE). A lack of knowledge regarding the mechanisms of thalidomide teratogenesis acts as a barrier in the aim to synthesize a safer analogue of thalidomide. Recently, our group detected a higher frequency of alleles that impair the pro-angiogenic mechanisms of endothelial nitric oxide synthase (eNOS), coded by the NOS3 gene. In this study we evaluated variable number tandem repeats (VNTR) functional polymorphism in intron 4 of NOS3 in individuals with TE (38) and Brazilians without congenital anomalies (136). Haplotypes were estimated for this VNTR with previously analyzed polymorphisms, rs2070744 (-786C > T) and rs1799983 (894T > G), in promoter region and exon 7, respectively. Haplotypic distribution was different between the groups (p = 0.007). Alleles -786C (rs2070744) and 4b (VNTR), associated with decreased NOS3 expression, presented in higher frequency in TE individuals (p = 0.018; OR = 2.57; IC = 1.2-5.8). This association was not identified with polymorphism 894T > G (p = 0.079), which influences eNOS enzymatic activity. These results suggest variants in NOS3, with pre-transcriptional effects as susceptibility factors, influencing the risk TE development. This finding generates insight for a new approach to research that pursues a safer analogue.

  12. Sprint interval and endurance training are equally effective in increasing muscle microvascular density and eNOS content in sedentary males.

    Science.gov (United States)

    Cocks, Matthew; Shaw, Christopher S; Shepherd, Sam O; Fisher, James P; Ranasinghe, Aaron M; Barker, Thomas A; Tipton, Kevin D; Wagenmakers, Anton J M

    2013-02-01

    Sprint interval training (SIT) has been proposed as a time efficient alternative to endurance training (ET) for increasing skeletal muscle oxidative capacity and improving certain cardiovascular functions. In this study we sought to make the first comparisons of the structural and endothelial enzymatic changes in skeletal muscle microvessels in response to ET and SIT. Sixteen young sedentary males (age 21 ± SEM 0.7 years, BMI 23.8 ± SEM 0.7 kg m(-2)) were randomly assigned to 6 weeks of ET (40-60 min cycling at ∼65% , 5 times per week) or SIT (4-6 Wingate tests, 3 times per week). Muscle biopsies were taken from the m. vastus lateralis before and following 60 min cycling at 65% to measure muscle microvascular endothelial eNOS content, eNOS serine(1177) phosphorylation, NOX2 content and capillarisation using quantitative immunofluorescence microscopy. Whole body insulin sensitivity, arterial stiffness and blood pressure were also assessed. ET and SIT increased skeletal muscle microvascular eNOS content (ET 14%; P < 0.05, SIT 36%; P < 0.05), with a significantly greater increase observed following SIT (P < 0.05). Sixty minutes of moderate intensity exercise increased eNOS ser(1177) phosphorylation in all instances (P < 0.05), but basal and post-exercise eNOS ser(1177) phosphorylation was lower following both training modes. All microscopy measures of skeletal muscle capillarisation (P < 0.05) were increased with SIT or ET, while neither endothelial nor sarcolemmal NOX2 was changed. Both training modes reduced aortic stiffness and increased whole body insulin sensitivity (P < 0.05). In conclusion, in sedentary males SIT and ET are effective in improving muscle microvascular density and eNOS protein content.

  13. New development of ion exchange resin application. Ion kokan jushi oyo no aratana tenkai

    Energy Technology Data Exchange (ETDEWEB)

    Terayama, H. (Nippon Rensui Co., Tokyo (Japan))

    1990-09-01

    Ion exchange resin, already widely used, was introduced particularly in recent application examples to have utilized other functions than that of ion exchange. Purposewise, demineralized water production and other water treatment use occupies about a 70% share. Though super-demineralized water production unit, functionally based on ion exchange, has come to be able to produce super-demineralized water, more than 18MOmega-cm in specific resistivity, even a small quantity of eluent matter is so problematic as to require continuation of research to materialize further high purity. Recent chromatographic separation, as large-scaled, also industrially, by the high grade separation technology of preudo-moving bed, quasi-continuation and other systems, and development of new resin, includes the separation of fruit sugar and grape super at the largest scale. Examples comprise, among many other, hard ion exchange resin, as protein-separating agent, to replace conventional dextran, ion exchange electrolysis membrane, by chelate resin, of caustic soda and separation/refining of antibiotics by synthesizing/absorbing agent. 5 refs., 7 figs., 3 tabs.

  14. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus Otto; Mortensen, A; Nilas, Lisbeth

    2007-01-01

    . Concentration response curves to carbamylcholine chloride, sodium nitroprusside (SNP) and l-NAME were evaluated after precontraction with potassium. Total RNA was extracted, reverse transcribed and eNOS quantitated by real-time polymerase chain reaction (real-time PCR). RESULTS: Plasma cholesterol...... in any of the arteries. Correspondingly, eNOS mRNA was similarly expressed in all treatment groups in both arteries. CONCLUSIONS: Improvement of cerebral endothelial function by estrogen or soy isoflavones in ovariectomized WHHL rabbits is not supported by the present data. The findings may be unique...

  15. Circulating blood endothelial nitric oxide synthase contributes to the regulation of systemic blood pressure and nitrite homeostasis.

    Science.gov (United States)

    Wood, Katherine C; Cortese-Krott, Miriam M; Kovacic, Jason C; Noguchi, Audrey; Liu, Virginia B; Wang, Xunde; Raghavachari, Nalini; Boehm, Manfred; Kato, Gregory J; Kelm, Malte; Gladwin, Mark T

    2013-08-01

    Mice genetically deficient in endothelial nitric oxide synthase (eNOS(-/-)) are hypertensive with lower circulating nitrite levels, indicating the importance of constitutively produced nitric oxide (NO•) to blood pressure regulation and vascular homeostasis. Although the current paradigm holds that this bioactivity derives specifically from the expression of eNOS in endothelium, circulating blood cells also express eNOS protein. A functional red cell eNOS that modulates vascular NO• signaling has been proposed. To test the hypothesis that blood cells contribute to mammalian blood pressure regulation via eNOS-dependent NO• generation, we cross-transplanted wild-type and eNOS(-/-) mice, producing chimeras competent or deficient for eNOS expression in circulating blood cells. Surprisingly, we observed a significant contribution of both endothelial and circulating blood cell eNOS to blood pressure and systemic nitrite levels, the latter being a major component of the circulating NO• reservoir. These effects were abolished by the NOS inhibitor L-NG-nitroarginine methyl ester and repristinated by the NOS substrate L-arginine and were independent of platelet or leukocyte depletion. Mouse erythrocytes were also found to carry an eNOS protein and convert (14)C-arginine into (14)C-citrulline in NOS-dependent fashion. These are the first studies to definitively establish a role for a blood-borne eNOS, using cross-transplant chimera models, that contributes to the regulation of blood pressure and nitrite homeostasis. This work provides evidence suggesting that erythrocyte eNOS may mediate this effect.

  16. Xuezhikang, extract of red yeast rice, improved abnormal hemorheology, suppressed caveolin-1 and increased eNOS expression in atherosclerotic rats.

    Directory of Open Access Journals (Sweden)

    Xin-Yuan Zhu

    Full Text Available BACKGROUND: Xuezhikang is the extract of red yeast rice, which has been widely used for the management of atherosclerotic disease, but the molecular basis of its antiatherosclerotic effects has not yet been fully identified. Here we investigated the changes of eNOS in vascular endothelia and RBCs, eNOS regulatory factor Caveolin-1 in endothelia, and hemorheological parameters in atherosclerotic rats to explore the protective effects of Xuezhikang. METHODOLOGY/PRINCIPAL FINDINGS: Wistar rats were divided into 4 groups (n = 12/group group C, controls; group M, high-cholesterol diet (HCD induced atherosclerotic models; group X, HCD+Xuezhikang; and group L, HCD +Lovastatin. In group X, Xuezhikang inhibited oxidative stress, down-regulated caveolin-1 in aorta wall (P<0.05, up-regulated eNOS expression in vascular endothelia and erythrocytes (P<0.05, increased NOx (nitrite and nitrate in plasma and cGMP in erythrocyte plasma and aorta wall (P<0.05, increased erythrocyte deformation index (EDI, and decreased whole blood viscosity and plasma viscosity (P<0.05, with the improvement of arterial pathology. CONCLUSIONS/SIGNIFICANCE: Xuezhikang up-regulated eNOS expression in vascular endothelia and RBCs, increased plasma NOx and improved abnormal hemorheology in high cholesterol diet induced atherosclerotic rats. The elevated eNOS/NO and improved hemorheology may be beneficial to atherosclerotic disease.

  17. Disruption of COX-2 and eNOS does not confer protection from cardiovascular failure in lipopolysaccharide-treated conscious mice and isolated vascular rings

    DEFF Research Database (Denmark)

    Stæhr, Mette; Madsen, Kirsten; Vanhoutte, Paul M

    2011-01-01

    blood pressure (MAP) and heart rate (HR) that was significant after 3 h and was sustained throughout the experiment (8 h). The LPS-induced changes in MAP and HR were not different from control in COX-2(-/-) and eNOS(-/-) mice. A prostacyclin receptor antagonist (BR5064) blocked the hypotensive effect...

  18. Chronic aerobic exercise associated to dietary modification improve endothelial function and eNOS expression in high fat fed hamsters.

    Directory of Open Access Journals (Sweden)

    Beatriz C S Boa

    Full Text Available Obesity is epidemic in the western world and central adipose tissue deposition points to increased cardiovascular morbidity and mortality, independently of any association between obesity and other cardiovascular risk factors. Physical exercise has been used as non-pharmacological treatment to significantly reverse/attenuate obesity comorbidities. In this study we have investigated effects of exercise and/or dietary modification on microcirculatory function, body composition, serum glucose, iNOS and eNOS expression on 120 male hamsters treated for 12 weeks with high fat chow (HF, n = 30 starting on the 21st day of birth. From week 12 to 20, animals were randomly separated in HF (no treatment change, return to standard chow (HFSC, n = 30, high fat chow associated to an aerobic exercise training program (AET (HFEX, n = 30 and return to standard chow+AET (HFSCEX, n = 30. Microvascular reactivity in response to acetylcholine and sodium nitroprusside and macromolecular permeability increase induced by 30 minutes ischemia followed by reperfusion were assessed on the cheek pouch preparation. Total body fat and aorta eNOS and iNOS expression by immunoblotting assay were evaluated on the experimental day. Compared to HFSC and HFSCEX groups, HF and HFEX ones presented increased visceral fat [(mean±SEM (HF4.9±1.5 g and (HFEX4.7±0.9 g vs. (HFSC*3.0±0.7 g and (HFSCEX*1.9±0.4 g/100 g BW]; impaired endothelial-dependent vasodilatation [Ach 10(-8 M (HF87.9±2.7%; (HFSC*116.7±5.9%; (HFEX*109.1±4.6%; (HFSCEX*105±2.8%; Ach10(-6 M (HF95.3±3.1%; (HFSC*126±6.2%; (HFEX*122.5±2.8%; (HFSCEX*118.1±4.3% and Ach10(-4 M (HF109.5±4.8%; (HFSC*149.6±6.6%; (HFEX*143.5±5.4% and (HFSCEX*139.4±5.2%], macromolecular permeability increase after ischemia/reperfusion [(HF40.5±4.2; (HFSC*19.0±1.6; (HFEX*18.6±2.1 and (HFSCEX* 21.5±3.7 leaks/cm2, decreased eNOS expression, increased leptin and glycaemic levels. Endothelial-independent microvascular

  19. Chronic aerobic exercise associated to dietary modification improve endothelial function and eNOS expression in high fat fed hamsters.

    Science.gov (United States)

    Boa, Beatriz C S; Souza, Maria das Graças C; Leite, Richard D; da Silva, Simone V; Barja-Fidalgo, Thereza Christina; Kraemer-Aguiar, Luiz Guilherme; Bouskela, Eliete

    2014-01-01

    Obesity is epidemic in the western world and central adipose tissue deposition points to increased cardiovascular morbidity and mortality, independently of any association between obesity and other cardiovascular risk factors. Physical exercise has been used as non-pharmacological treatment to significantly reverse/attenuate obesity comorbidities. In this study we have investigated effects of exercise and/or dietary modification on microcirculatory function, body composition, serum glucose, iNOS and eNOS expression on 120 male hamsters treated for 12 weeks with high fat chow (HF, n = 30) starting on the 21st day of birth. From week 12 to 20, animals were randomly separated in HF (no treatment change), return to standard chow (HFSC, n = 30), high fat chow associated to an aerobic exercise training program (AET) (HFEX, n = 30) and return to standard chow+AET (HFSCEX, n = 30). Microvascular reactivity in response to acetylcholine and sodium nitroprusside and macromolecular permeability increase induced by 30 minutes ischemia followed by reperfusion were assessed on the cheek pouch preparation. Total body fat and aorta eNOS and iNOS expression by immunoblotting assay were evaluated on the experimental day. Compared to HFSC and HFSCEX groups, HF and HFEX ones presented increased visceral fat [(mean±SEM) (HF)4.9±1.5 g and (HFEX)4.7±0.9 g vs. (HFSC)*3.0±0.7 g and (HFSCEX)*1.9±0.4 g/100 g BW]; impaired endothelial-dependent vasodilatation [Ach 10(-8) M (HF)87.9±2.7%; (HFSC)*116.7±5.9%; (HFEX)*109.1±4.6%; (HFSCEX)*105±2.8%; Ach10(-6) M (HF)95.3±3.1%; (HFSC)*126±6.2%; (HFEX)*122.5±2.8%; (HFSCEX)*118.1±4.3% and Ach10(-4) M (HF)109.5±4.8%; (HFSC)*149.6±6.6%; (HFEX)*143.5±5.4% and (HFSCEX)*139.4±5.2%], macromolecular permeability increase after ischemia/reperfusion [(HF)40.5±4.2; (HFSC)*19.0±1.6; (HFEX)*18.6±2.1 and (HFSCEX)* 21.5±3.7 leaks/cm2), decreased eNOS expression, increased leptin and glycaemic levels. Endothelial

  20. A Multilevel Prediction of Physiological Response to Challenge: Interactions among Child Maltreatment, Neighborhood Crime, eNOS and GABRA6

    Science.gov (United States)

    Lynch, Michael; Manly, Jody Todd; Cicchetti, Dante

    2015-01-01

    Physiological response to stress has been linked to variety of healthy and pathological conditions. The current study conducted a multilevel examination of interactions among environmental toxins – i.e., neighborhood crime and child maltreatment – and specific genetic polymorphisms of eNOS and GABRA6. A total of 186 children were recruited at age 4. At this time, the presence or absence of child maltreatment was determined, as was the amount of crime that occurred in their neighborhood during the previous year. At age 9, the children were brought to the lab where their physiological response to a cognitive challenge – i.e., change in the amplitude of RSA – was assessed and DNA samples were collected for subsequent genotyping. Results confirmed that complex G x G, E x E, and G x E interactions were associated with different patterns of RSA reactivity. The implications for future research and evidence-based intervention are discussed. PMID:26535938

  1. Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis.

    Science.gov (United States)

    Boopathy, Gandhi T K; Kulkarni, Madhura; Ho, Sze Yuan; Boey, Adrian; Chua, Edmond Wei Min; Barathi, Veluchamy A; Carney, Tom J; Wang, Xiaomeng; Hong, Wanjin

    2017-10-27

    Angiogenesis is a highly regulated process for formation of new blood vessels from pre-existing ones. Angiogenesis is dysregulated in various pathologies, including age-related macular degeneration, arthritis, and cancer. Inhibiting pathological angiogenesis therefore represents a promising therapeutic strategy for treating these disorders, highlighting the need to study angiogenesis in more detail. To this end, identifying the genes essential for blood vessel formation and elucidating their function are crucial for a complete understanding of angiogenesis. Here, focusing on potential candidate genes for angiogenesis, we performed a morpholino-based genetic screen in zebrafish and identified Cavin-2, a membrane-bound phosphatidylserine-binding protein and critical organizer of caveolae (small microdomains in the plasma membrane), as a regulator of angiogenesis. Using endothelial cells, we show that Cavin-2 is required for in vitro angiogenesis and also for endothelial cell proliferation, migration, and invasion. We noted a high level of Cavin-2 expression in the neovascular tufts in the mouse model of oxygen-induced retinopathy, suggesting a role for Cavin-2 in pathogenic angiogenesis. Interestingly, we also found that Cavin-2 regulates the production of nitric oxide (NO) in endothelial cells by controlling the stability and activity of the endothelial nitric-oxide synthase (eNOS) and that Cavin-2 knockdown cells produce much less NO than WT cells. Also, mass spectrometry, flow cytometry, and electron microscopy analyses indicated that Cavin-2 is secreted in endothelial microparticles (EMPs) and is required for EMP biogenesis. Taken together, our results indicate that in addition to its function in caveolae biogenesis, Cavin-2 plays a critical role in endothelial cell maintenance and function by regulating eNOS activity. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  2. Alteration in cardiac uncoupling proteins and eNOS gene expression following high-intensity interval training in favor of increasing mechanical efficiency

    Science.gov (United States)

    Fallahi, Ali Asghar; Shekarfroush, Shahnaz; Rahimi, Mostafa; Jalali, Amirhossain; Khoshbaten, Ali

    2016-01-01

    Objective(s): High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Materials and Methods: Wistar rats were divided into five groups: control group (n=12), HIIT for an acute bout (AT1), short term HIIT for 3 and 5 sessions (ST3 and ST5), long-term training for 8 weeks (LT) (6 in each group). The rats of the training groups were made to run on a treadmill for 60 min in three stages: 6 min running for warm-up, 7 intervals of 7 min running on treadmill with a slope of 5° to 20° (4 min with an intensity of 80-110% VO2max and 3 min at 50-60% VO2max), and 5-min running for cool-down. The control group did not participate in any exercise program. Rats were sacrificed and the hearts were extracted to analyze the levels of UCP2, UCP3 and eNOS mRNA by RT-PCR. Results: UCP3 expression was increased significantly following an acute training bout. Repeated HIIT for 8 weeks resulted in a significant decrease in UCPs mRNA and a significant increase in eNOS expression in cardiac muscle. Conclusion: This study indicates that Long term HIIT through decreasing UCPs mRNA and increasing eNOS mRNA expression may enhance energy efficiency and physical performance. PMID:27114795

  3. Work-related stress perception and hypertension amongst health workers of a mission hospital in Oyo State, south-western Nigeria

    Directory of Open Access Journals (Sweden)

    Akinwumi O. Owolabi

    2012-02-01

    Full Text Available Background: Globalisation and changes in the nature of work have resulted in increasing work-related stress in people in developing countries. Work stress is at present already acknowledged as one of the epidemics of modern working life. It is associated with a number of disease conditions, such as hypertension, cardiovascular diseases, affective disorders, depression, disturbed metabolism (risk of Type II diabetes and musculoskeletal disorders.Objective: This study was a work site cross-sectional descriptive study carried out amongst the health workers at the Baptist Medical Centre Ogbomoso, Oyo State, south-western Nigeria. The aim of the study was to discern the prevalence of perceived work stress and to explore the relationship between perceived work stress and the presence of hypertension.Methods: A total of 324 consenting health workers of the institution were administered the job demand-control questionnaire to assess work stress. A standardised questionnaire was used to collect socio-demographic data and other personal data. Measurements of blood pressure, weight and height were carried out and body mass indices were calculated.Results: More than a quarter (26.2% of the subjects perceived themself as stressed at work. The single largest group of hypertensive subjects was seen amongst subjects with work stress.Conclusion: A significant number of health workers in this study is afflicted by work-related stress and perceived work stress was found to be significantly associated with higher hypertension prevalence.

  4. Association of eNOS polymorphisms with susceptibility to osteonecrosis of the femur head : A meta-analysis.

    Science.gov (United States)

    Song, G G; Lee, Y H

    2017-04-01

    The aim of the present study was to determine whether polymorphisms of the endothelial nitric oxide synthase (eNOS) gene are associated with susceptibility to osteonecrosis of the femoral head (ONFH). We conducted a meta-analysis to assess the association between the 4b/a, G894T, and T786C polymorphisms of eNOS and the susceptibility to ONFH. A total of five studies, which included 566 cases and 833 controls, were included in the meta-analysis. Meta-analysis revealed a significant association between allele a of the 4b/a polymorphism and ONFH in all study subjects (odds ratio, OR 3.237; 95 % confidence interval, CI 2.036-5.148; P = 6.9 × 10-7); stratification by ethnicity indicated an association between this allele and ONFH in Caucasians and Asians (OR 2.985; 95 % CI 1.592-5.597; P = 0.001 and OR 3.567; 95 % CI 1.793-7.095; P = 2.9 × 10-4, respectively). Meta-analysis stratified by ONFH type showed a significant association between allele a of the 4b/a polymorphism and idiopathic and secondary ONFH (OR 3.411; 95 % CI 2.049-5.679; P = 2.4 × 10-6 and OR 3.163; 95 % CI 1.781-5.619, P = 8.6 × 10 -5, respectively). However, the meta-analysis did not show any allelic association between the G894T and T786C polymorphisms and ONFH (OR 1.718; 95 % CI 0.796-3.707; P = 0.168 and OR 1.027; 95 % CI 0.191-5.517; P = 0.976, respectively). Our meta-analysis of published studies shows that the 4b/a polymorphism is associated with the development of idiopathic and secondary ONFH in Caucasians and Asians.

  5. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus Otto; Mortensen, A; Nilas, Lisbeth

    2007-01-01

    OBJECTIVES: To evaluate the effect of estrogen replacement therapy or soy isoflavones supplement on endothelium-dependent relaxation in vitro and gene expression of endothelial nitric oxide synthase (eNOS) in cerebral arteries in a rabbit model of human hypercholesterolemia. STUDY DESIGN: Thirty....... Concentration response curves to carbamylcholine chloride, sodium nitroprusside (SNP) and l-NAME were evaluated after precontraction with potassium. Total RNA was extracted, reverse transcribed and eNOS quantitated by real-time polymerase chain reaction (real-time PCR). RESULTS: Plasma cholesterol...... was significantly higher at termination in the SoyLife group (Pendothelium-dependent responses to carbamylcholine chloride or l-NAME or the endothelium-independent response to SNP...

  6. What correlation does the electron structure of environmental hormones have with manifestation of their toxicity and biological activity. Application of the hardness conception to biology; Kankyo horumon no denshi kozo wa sono dokusei ya seibutsu kassei no hatsugen ni donoyona sokansei wo motsunode aroka. Hardness gainen no seibutsugaku eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, S.; Tanaka, A. [Showa College of Pharmaceutical Sciences, Tokyo (Japan); Sameshima, K. [Fujitsu Ltd., Tokyo (Japan)

    1999-01-01

    This paper indicates that the relationship between toxicity and biological activity of environmental hormones such as dioxin and their chemical structures may be predicted by using parameters (physical quantity) derived from the chemical hardness conception. According to this conception, softness and hardness of molecules are important in addition to electron receptivity and donation properties of molecules. Soft compounds facilitate electrons to migrate. Chlorine displacement of dioxin skeleton to 2nd, 3rd, 7th and 8th orders softens the molecules and displacement to 1st, 4th, 6th and 9th orders hardens them. The toxicity and biological activity of the environmental hormones cannot be explained only with the fit model of key-keyhole with the counterpart they act on (receptor). The softer the chemical hardness, the greater the toxicity and biological activity. Development has been made on an absolute hardness versus absolute electrical negative activity diagram to learn electron structure of molecules. This diagram indicates that the toxicity of dioxin depends more strongly on the chemical hardness than the strength of the electrical negativity, and the softer the hardness, the stronger the toxicity. (NEDO)

  7. Fiscal 1998 achievement report on regional consortium research and development project. Venture business raising type regional consortium - small business creating base type (Control of gene expression by ligands for nuclear receptors and its application to medicine manufacture - 2nd year); 1998 nendo kakunai juyotai ligand ni yoru iden joho hatsugen no seigyo to iyaku seizo process eno oyo seika hokokusho. 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    Studies are made about ascochlorin and its derivatives which are expected to be effective in the treatment and prevention of lifestyle diseases such as arteriosclerosis, diabetes, hypertension, etc. The current goal is to definitely prove at the cell culture stage that ascochlorin and its derivatives act as ligands for nuclear receptors. As the result of the effort to prove their activation of nuclear receptors, it is clarified that they activate not only PPARr, which is the initial target of the research, but also PPARa, PXR, and ER. A computer simulation of interaction between ascochlorin derivatives and PPARr is conducted, and now it is predicted that the helix 10 cubic structure is transformed so that co-activators may connect to the structure. It is also found that AS-6 inhibits almost completely the appearance of type II diabetes in the db/db mouse lacking leptin receptors and type I diabetes in the NOD (non-obese diabetes) mouse. (NEDO)

  8. Acoustic excitation of diffusion flames with coherent structure in a plane shear layer. ; Application of active combustion control to two-dimensional phase-locked arranging measurements. Soshiki kozo wo tomonau heimen sendai kakusan kaen no onkyo reiki. ; Nijigen iso heikin bunpu sokutei eno active nensho seigyo no oyo

    Energy Technology Data Exchange (ETDEWEB)

    Ishino, Y.; Kojima, T.; Oiwa, N.; Yamaguchi, S. (Nagoya Institute of Technology, Nagoya (Japan))

    1993-11-25

    The acoustic excitation of a plane diffusion flame enhances the periodicity of organized eddy controlled combustion. In this study, to clarify an effectiveness of application of active combustion control, phase characteristics of the excited eddy flames with high periodicity have been examined. A computer-aided phase-locked averaging method was employed to obtain graphical two-dimensional contour maps of the instantaneous profiles of temperature and CH emission. Both maps consisting of eight consecutive phases indicated clearly not only the periodic behavior of the organized eddy flame, but also the gas dynamic properties peculiar to those flames with coherent structure. In addition, the profiles of local contribution of the sound field to the combustion process were examined by calculating the two-dimensional distribution of the local Rayleigh index. Calculation results of the two-dimensional distribution of the local Rayleigh index indicated that the organized eddy flames have high sensitivity to sound, and play an important role in an interaction of sound and flame. 6 refs., 9 figs.

  9. FY 1995 development of fluorinated hydriding alloys with multi functional and functionally-graded surface and their application to energy conversion devices; 1995 nendo keishagata fukugo kino wo hyomen ni motsu suiso kyuzo gokin no kaihatsu to energy henkan gijutsu eno oyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The project is the extended researches of a fluorination technique invented by the project leader (Suda) for improving the surface properties and characteristics of conventional hydriding alloys from the following viewpoints; (1) To investigate the roles of fluorinated surface during hydrogen uptake both in the gas-solid and the electrochemical reactions. (2) To elucidate the factors which increase the protective nature of the surface. (3) To develop a material design procedure for synthesizing thin layer of functionally graded surface which is composed of metallic Ni and the fluoride compound. (1) An advanced fluorination technique was developed to incorporate metallic Ni in the surface fluoride layer. (2) Metallic Ni was successfully distributed in a functionally graded manner in the Surface fluoride layer. (3) Through the technique developed, the following properties and characteristics were successfully donated in the fluorinated hydriding alloys such as AB{sub 5}, AB{sub 2}, and AB; (3-1)Surface oxides which act as the resistant layer to the hydrogen up take was completely eliminated to result in the enhancement of the initial activation characteristics. (3-2) Hydrogen selectivity and permeability was greatly improved. (3-3) Surface protective nature against the impurity gases and contaminants was significantly improved. (3-4) Initial activation characteristics both in the gas-solid and the electrochemical reactions were distinguishably improved. (3-5) Fluorinated surface was found to function as the catalyst for a methanation reaction between the CO{sub 2} gas adsorbed over the fluorinated surface and the monatomic hydrogen absorbed in the metal lattice of the crystalline structure of the hydriding alloys. (4) A technique was developed for increasing the specific surface area and decreasing the specific surface diameter of the fluorinated hydriding alloy articles. (NEDO)

  10. Upregulation of ERK1/2-eNOS via AT2 receptors decreases the contractile response to angiotensin II in resistance mesenteric arteries from obese rats.

    Directory of Open Access Journals (Sweden)

    Graziela N Hagihara

    Full Text Available It has been clearly established that mitogen-activated protein kinases (MAPKS are important mediators of angiotensin II (Ang II signaling via AT1 receptors in the vasculature. However, evidence for a role of these kinases in changes of Ang II-induced vasoconstriction in obesity is still lacking. Here we sought to determine whether vascular MAPKs are differentially activated by Ang II in obese animals. The role of AT2 receptors was also evaluated. Male monosodium glutamate-induced obese (obese and non-obese Wistar rats (control were used. The circulating concentrations of Ang I and Ang II, determined by HPLC, were increased in obese rats. Ang II-induced isometric contraction was decreased in endothelium-intact resistance mesenteric arteries from obese compared with control rats and exhibited a retarded AT1 receptor antagonist response. Blocking of AT2 receptors and inhibition of either endothelial nitric oxide synthase (eNOS or extracellular signal-regulated protein kinases 1 and 2 (ERK1/2 restored Ang II-induced contraction in obese rats. Western blot analysis revealed increased protein expression of AT2 receptors in arteries from obese rats. Basal and Ang II-induced ERK1/2 phosphorylation was also increased in obese rats. Blockade of either AT1 or AT2 receptors corrected the increased ERK1/2 phosphorylation in arteries from obese rats to levels observed in control preparations. Phosphorylation of eNOS was increased in obese rats. Incubation with the ERK1/2 inhibitor before Ang II stimulation did not affect eNOS phosphorylation in control rats; however, it corrected the increased phosphorylation of eNOS in obese rats. These results clearly demonstrate that enhanced AT2 receptor and ERK1/2-induced, NO-mediated vasodilation reduces Ang II-induced contraction in an endothelium-dependent manner in obese rats.

  11. Upregulation of ERK1/2-eNOS via AT2 receptors decreases the contractile response to angiotensin II in resistance mesenteric arteries from obese rats.

    Science.gov (United States)

    Hagihara, Graziela N; Lobato, Nubia S; Filgueira, Fernando P; Akamine, Eliana H; Aragão, Danielle S; Casarini, Dulce E; Carvalho, Maria Helena C; Fortes, Zuleica B

    2014-01-01

    It has been clearly established that mitogen-activated protein kinases (MAPKS) are important mediators of angiotensin II (Ang II) signaling via AT1 receptors in the vasculature. However, evidence for a role of these kinases in changes of Ang II-induced vasoconstriction in obesity is still lacking. Here we sought to determine whether vascular MAPKs are differentially activated by Ang II in obese animals. The role of AT2 receptors was also evaluated. Male monosodium glutamate-induced obese (obese) and non-obese Wistar rats (control) were used. The circulating concentrations of Ang I and Ang II, determined by HPLC, were increased in obese rats. Ang II-induced isometric contraction was decreased in endothelium-intact resistance mesenteric arteries from obese compared with control rats and exhibited a retarded AT1 receptor antagonist response. Blocking of AT2 receptors and inhibition of either endothelial nitric oxide synthase (eNOS) or extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) restored Ang II-induced contraction in obese rats. Western blot analysis revealed increased protein expression of AT2 receptors in arteries from obese rats. Basal and Ang II-induced ERK1/2 phosphorylation was also increased in obese rats. Blockade of either AT1 or AT2 receptors corrected the increased ERK1/2 phosphorylation in arteries from obese rats to levels observed in control preparations. Phosphorylation of eNOS was increased in obese rats. Incubation with the ERK1/2 inhibitor before Ang II stimulation did not affect eNOS phosphorylation in control rats; however, it corrected the increased phosphorylation of eNOS in obese rats. These results clearly demonstrate that enhanced AT2 receptor and ERK1/2-induced, NO-mediated vasodilation reduces Ang II-induced contraction in an endothelium-dependent manner in obese rats.

  12. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits

    DEFF Research Database (Denmark)

    Lund, Claus O.; Mortensen, Alicja; Nilas, Lisbeth

    2007-01-01

    recordings. Concentration response curves to carbamylcholine chloride, sodium nitroprusside (SNP) and L-NAME were evaluated after precontraction with potassium. Total RNA was extracted, reverse transcribed and eNOS quantitated by real-time polymerase chain reaction (real-time PCR). Results: Plasma...... be unique to the WHHL rabbit in which the hypocholesterolemic effect of estrogens mediated by upregulation of liver LDL receptors is excluded....

  13. eNOS overexpressing bone marrow cells are safe and effective in a porcine model of myocardial regeneration following acute myocardial infarction.

    Science.gov (United States)

    Ward, Michael R; Connelly, Kim A; Vijayaraghavan, Ram; Vaags, Andrea K; Graham, John J; Foltz, Warren; Hough, Margaret R; Stewart, Duncan J; Dick, Alexander

    2013-12-01

    Cell therapy has been shown to be effective in improving LV function postmyocardial infarction (MI). We hypothesized that eNOS-transfected bone marrow cells (BMCs) are safe in a swine model of myocardial infarction (MI). We also hypothesized that endothelial nitric oxide synthase (eNOS) transfection would enhance cell function, as assessed by myocardial functional recovery post-MI. Fifteen female Yorkshire pigs underwent bone marrow aspiration and creation of MI. Bone marrow cells were cultured for 7 days, and each pig received either autologous BMCs transiently transfected with eNOS plasmid (eNOS-BMC, n = 5), nontransfected BMCs (nt-BMC, n = 4), or phosphate-buffered saline (PBS) control (n = 6). Cardiac MRI was performed at baseline (1 week post-MI) and 6 weeks post-MI. There was no difference in safety outcomes between groups. Absolute left ventricular ejection fraction (LVEF) at 6 weeks showed a trend toward improvement in both cell therapy groups compared with baseline but worsened in the PBS control group. The absolute improvement in LVEF was significantly greater in both cell therapy groups compared with PBS control. Infarct mass was significantly lower in the eNOS-BMC group between baseline and 6 weeks, but the absolute change in infarct mass was not different between groups. Finally, there was a trend toward reduced LV mass in the eNOS-BMC group. Bone marrow cell delivery, with and without eNOS overexpression, is safe and leads to improvement in LVEF when administered in the coronary circulation 7 days following acute MI in swine. Transfection of healthy BMCs with eNOS resulted in some improvement in left ventricular remodeling. Further study is warranted in a preclinical model that approximates the impact of cardiovascular risk factors on BMC function. © 2013 John Wiley & Sons Ltd.

  14. Quercetin modulates iNOS, eNOS and NOSTRIN expressions and attenuates oxidative stress in warm hepatic ischemia-reperfusion injury in rats

    Directory of Open Access Journals (Sweden)

    Mohamed Abd-Elbaset

    2015-09-01

    Full Text Available The pathogenesis of hepatic ischemia/reperfusion (I/R is mediated through the generation of oxidative and nitrosative stress-induced cell injury. Hence, the present study was designed to evaluate the hepatoprotective effect of quercetin (QR compared to N-acetylcysteine (NAC against hepatic I/R injury in rats and to assess iNOS, eNOS and NOSTRIN protein expressions, as a possible mechanism of its hepatoprotective effect. Hepatic ischemia was surgically performed by occlusion of hepatic pedicle (hepatic artery, portal vein, bile duct that supplies the left and medial lobes (approximately 70% of the total liver mass, for 30 min with a vascular clamp followed by releasing the clamp and the liver was reperfused for 30 min. QR-pretreatment increased eNOS protein expression with simultaneous decrease in iNOS and NOSTRIN protein expressions. It also decreased serum aspartate aminotransferase (AST, alanine aminotransferases (ALT and hepatic myeloperoxidase (MPO activities. In addition, it restored the depleted content of reduced glutathione (GSH and decreased malondialdehyde (MDA and nitric oxide (NO levels. A notable finding is that QR alleviated I/R-induced histopathological changes. Therefore, the present study illustrates the hepatoprotective effect of quercetin compared to N-acetylcysteine against ischemia/reperfusion-induced liver injury by inhibiting oxidative stress and by modulating iNOS, eNOS and NOSTRIN protein expressions.

  15. Association between eNOS 4b/a Polymorphism and the Risk of Diabetic Retinopathy in Type 2 Diabetes Mellitus: A Meta-Analysis

    Directory of Open Access Journals (Sweden)

    Ze-jun Ma

    2014-01-01

    Full Text Available Many studies have assessed the association between eNOS-4b/a polymorphism and the risk of diabetic retinopathy (DR among type 2 diabetic subjects. However, the results are inconsistent. In order to derive a more precise estimation of the association, a meta-analysis was conducted. Fifteen studies with 3, 183 cases and 3, 410 controls were enrolled by searching the databases of Pubmed, Embase, China National Knowledge Infrastructure (CNKI, and Chinese Wanfang Database. Summary odds ratios (ORs with 95% confidence intervals (CIs were calculated. The main analysis indicated no significant association between eNOS-4b/a polymorphism and the risk of DR in overall population [allelic model: OR=0.94 (0.79–1.11; additive model: OR=0.91 (0.73–1.14; recessive model: OR=1.01 (0.81–1.25; dominant model: OR=0.91 (0.75–1.09]. Subgroup analysis by ethnicity also indicated no significant association. In conclusion, the current meta-analysis did not observe any association between the polymorphism of eNOS 4b/a and the risk of DR among type 2 diabetic subjects. However, larger well-designed studies are required to confirm this finding.

  16. Exercise Training Could Improve Age-Related Changes in Cerebral Blood Flow and Capillary Vascularity through the Upregulation of VEGF and eNOS

    Directory of Open Access Journals (Sweden)

    Sheepsumon Viboolvorakul

    2014-01-01

    Full Text Available This study aimed to investigate the effect of exercise training on age-induced microvascular alterations in the brain. Additionally, the association with the protein levels of vascular endothelial growth factor (VEGF and endothelial nitric oxide synthase (eNOS was also assessed. Male Wistar rats were divided into four groups: sedentary-young (SE-Young, n=5, sedentary aged (SE-Aged, n=8, immersed-aged (IM-Aged, n=5, and exercise trained-aged (ET-Aged, 60 minutes/day and 5 days/week for 8 weeks, n=8 rats. The MAPs of all aged groups, SE-Aged, IM-Aged, and ET-Aged, were significantly higher than that of the SE-Young group. The regional cerebral blood flow (rCBF in the SE-Aged and IM-Aged was significantly decreased as compared to SE-Young groups. However, rCBF of ET-Aged group was significantly higher than that in the IM-Aged group (P<0.05. Moreover, the percentage of capillary vascularity (%CV and the levels of VEGF and eNOS in the ET-Aged group were significantly increased compared to the IM-Aged group (P<0.05. These results imply that exercise training could improve age-induced microvascular changes and hypoperfusion closely associated with the upregulation of VEGF and eNOS.

  17. Water extract of Korean red ginseng stimulates angiogenesis by activating the PI3K/Akt-dependent ERK1/2 and eNOS pathways in human umbilical vein endothelial cells.

    Science.gov (United States)

    Kim, Young-Mi; Namkoong, Seung; Yun, Young-Gab; Hong, Hee-Do; Lee, Young-Chul; Ha, Kwon-Soo; Lee, Hansoo; Kwon, Ho Jeong; Kwon, Young-Guen; Kim, Young-Myeong

    2007-09-01

    Angiogenesis is important for promoting cardiovascular disease, wound healing, and tissue regeneration. We investigated the effects of Korean red ginseng water extract (KRGE) on angiogenesis and its underlying signal mechanism. KRGE increased in vitro proliferation, migration, and tube formation of human umbilical vein endothelial cells, as well as stimulated in vivo angiogenesis without increasing VEGF expression. KRGE-induced angiogenesis was accompanied by phosphorylation of ERK1/2, phosphatidylinositol 3-kinase (Akt), and endothelial nitric oxide synthase (eNOS) as well as an increase in NO production. Inhibition of PI3K activity by wortmannin completely inhibited KRGE-induced angiogenesis and phosphorylation of Akt, ERK1/2, and eNOS, indicating that PI3K/Akt activation is an upstream event of the KRGE-mediated angiogenic pathway. The MEK inhibitor PD98059 blocked KRGE-induced ERK1/2 phosphorylation without affecting Akt and eNOS activation. However, the eNOS inhibitor N(G)-monomethyl-L-arginine effectively inhibited tube formation, but partially blocked proliferation and migration as well as ERK phosphorylation, without altering Akt and eNOS activation, revealing that the eNOS/NO pathway is partially involved in ERK1/2 activation. This study demonstrated that KRGE stimulates in vitro and in vivo angiogenesis through the activation of the PI3K/Akt-dependent ERK1/2 and eNOS signal pathways and their cross talk.

  18. AKT3, ANGPTL4, eNOS3, and VEGFA associations with high altitude sickness in Han and Tibetan Chinese at the Qinghai-Tibetan Plateau.

    Science.gov (United States)

    Buroker, Norman E; Ning, Xue-Han; Zhou, Zhao-Nian; Li, Kui; Cen, Wei-Jun; Wu, Xiu-Feng; Zhu, Wei-Zhong; Scott, C Ronald; Chen, Shi-Han

    2012-08-01

    Mountain sickness (MS) occurs among humans visiting or inhabiting high altitude environments. We conducted genetic analyses of the AKT3, ANGPTL4, eNOS3 and VEGFA genes in lowland (Han) and highland (Tibetan) Chinese. Ten single nucleotide polymorphisms (SNPs) were evaluated in Han and Tibetan patients with acute (A) and chronic (C) MS. We compared 74 patients with AMS to 79 Han unaffected with MS, as well as 48 CMS patients to 31 unaffected Tibetans. The ten SNPs studied are AKT3 (rs4590656, rs2291409), ANGPTL4 (rs1044250), eNOS3 (rs1007311, rs1799983) and VEGFA (rs79469752, rs13207351, rs28357093, rs1570360, rs3025039). Direct sequencing was used to identify individual genotypes for these SNPs. Hemoglobin (Hb), hematocrit (Hct), and red blood cell count (RBC) were found to be significantly associated with the AKT3 SNP (rs4590656), Hb was found to be associated with the eNOS3 SNP (rs1007311), and RBC was found to be significantly associated with the VEGFA SNP (rs1570360) in Tibetan patients with CMS. CMS patients were found to diverge significantly for both eNOS3 SNPs as measured by genetic distance (0.042, 0.047) and for the VEGFA SNP (rs28357093) with a genetic distance of 0.078 compared to their Tibetan control group. Heart rate (HR) was found to be significantly associated with the eNOS3 SNP (rs1799983) and arterial oxygen saturation of hemoglobin (SaO2) was found to be significantly associated with the VEGFA SNPs (rs13207351, rs1570360) in Han patients with AMS. The Han and Tibetan control groups were found to diverge significantly for the ANGPTL4 SNP and VEGFA SNP (rs28357093), as measured by genetic distances of 0.049 and 0.073, respectively. Seven of the SNPs from non-coding regions are found in the transcriptional factor response elements and their possible role in gene regulation was evaluated with regard to MS. AMS and CMS were found to be significantly associated with the four genes compared to their Han and Tibetan control groups, respectively

  19. Prevalence of anti-hepatitis C virus antibody among pregnant women and blood donors at Bowen University Teaching Hospital, Ogbomoso, Oyo State, Nigeria.

    Science.gov (United States)

    Hilda, Awoyelu E; Kola, Oloke Julius; Kolawole, Oladipo E

    2017-01-01

    Hepatitis C virus is one of the emerging infectious diseases that can be transmitted through blood-to-blood contact. This study was carried out to determine the prevalence of anti-HCV antibodies among potential blood donors and pregnant women attending Bowen University Teaching Hospital (BUTH), Ogbomoso, Oyo State. This hospital-based study was conducted from December 2014 to September 2015. The study group (N = 279) included potential blood donors and pregnant women. Data on socio-demographic characteristics and potential risk factors were collected using a structured questionnaire. The presence of anti-HCV antibodies in serum samples of the studied subjects were detected using third-generation Enzyme Linked Immunosorbent Assay (ELISA) (WKEA Med Supplies Corp, China). Chisquare test was utilized to assess the association between the socio-demographic variables and HCV status. Logistic regression was done to determine the strength of association between risk factors and HCV status. Statistical significance was set at P ˂ 0.05. Overall seroprevalence of hepatitis C virus infection was found to be 1.79% consisting 0.36% of pregnant women and 1.43% of blood donors. None of the socio-demographic characteristics and potential risk factors among the study groups were significantly associated with hepatitis C virus infection. This study found a seroprevalence of anti-HCV antibody to be 1.79%, thus, screening of pregnant women and blood donors for HCV infections with the use of ELISA is recommended because of its important role in detecting the presence of anti-HCV antibody with utmost specificity and sensitivity.

  20. Biotechnological application of marine microalgae; Kaiyo sorui no biotechnology eno riyo

    Energy Technology Data Exchange (ETDEWEB)

    Matsunaga, T.; Takeyama, H. [Tokyo University of Agriculture and Technology, Tokyo (Japan). Faculty of Technology

    1995-09-10

    There are many kinds of micro algae who have adapted to extremely diversified marine environments. Screening of new useful materials from marine micro algae has been carried out. High density culture system using photo-bioreactor and genetic manipulation technology have been employed in order to enhance productivity of those materials from marine micro algae. We have found many useful materials, such as UV-A absorbing substance, plant growth regulating factor, polysaccharides, unsaturated fatty acids and antibiotics, from marine micro algae. Here, we describe production of those useful materials and their application. Marine coccolithophorid algae convert CO2 to CaCO3 and they have been expected to contribute to CO2 recycling system. Metabolic engineering in marine microalgae, especially cyanobacteria, has been established. Eicosapentaenoic acid is successfully synthesized in marine cyanobacterium which can not synthesize it. 41 refs., 14 figs., 5 tabs.

  1. MiR-21 is induced in endothelial cells by shear stress and modulates apoptosis and eNOS activity

    Energy Technology Data Exchange (ETDEWEB)

    Weber, Martina; Baker, Meredith B.; Moore, Jeffrey P. [Division of Cardiology, Emory University, 1639 Pierce Drive, WMB 319, Atlanta, GA 30322 (United States); Searles, Charles D., E-mail: csearle@emory.edu [Division of Cardiology, Emory University, 1639 Pierce Drive, WMB 319, Atlanta, GA 30322 (United States); Atlanta Veterans Administration Medical Center, 1670 Clarimont Road, Decatur, GA 30033 (United States)

    2010-03-19

    Mechanical forces associated with blood flow play an important role in regulating vascular signaling and gene expression in endothelial cells (ECs). MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. miRNAs are known to have an important role in modulating EC biology, but their expression and functions in cells subjected to shear stress conditions are unknown. We sought to determine the miRNA expression profile in human ECs subjected to unidirectional shear stress and define the role of miR-21 in shear stress-induced changes in EC function. TLDA array and qRT-PCR analysis performed on HUVECs exposed to prolonged unidirectional shear stress (USS, 24 h, 15 dynes/cm{sup 2}) identified 13 miRNAs whose expression was significantly upregulated (p < 0.05). The miRNA with the greatest change was miR-21; it was increased 5.2-fold (p = 0.002) in USS-treated versus control cells. Western analysis demonstrated that PTEN, a known target of miR-21, was downregulated in HUVECs exposed to USS or transfected with pre-miR-21. Importantly, HUVECs overexpressing miR-21 had decreased apoptosis and increased eNOS phosphorylation and nitric oxide (NO{sup {center_dot}}) production. These data demonstrate that shear stress forces regulate the expression of miRNAs in ECs, and that miR-21 influences endothelial biology by decreasing apoptosis and activating the NO{sup {center_dot}} pathway. These studies advance our understanding of the mechanisms by which shear stress forces modulate vascular homeostasis.

  2. Waste minimization activities in Toyota Motor Corporation; Haikibutsu teigen eno torikumi jokyo

    Energy Technology Data Exchange (ETDEWEB)

    Taguchi, S.; Yamamoto, N.; Kurai, H. [Toyota Motor Corp., Aichi (Japan)

    1998-07-01

    Toyota`s waste reduction efforts based on a drive for dealing with the source of waste generation are introduced. For the propulsion of the policy for dealing directly with the origin of waste, it is necessary to convert the existing manufacturing processes that give high priority to product quality and low production cost into environmental impact reduction oriented processes. In particular, conversion of materials into those that are easier to recycle, technology for reducing defective products, technology for the completion of recycling within the process involved, and the simplification of all the processes are mentioned. For the cleaning job in the machining process, a detergent enhanced in oil/water separating capability is developed, and this prolongs doubly the detergent replacement period and decreases the waste liquid to a 1/4 or less of what the conventional method produces. For the handling of foundry sand that is yielded in great quantities, a flow classification system is developed to replace the conventional gravitational classifier, and the reusable coarse-grain sand in the collected dust which occupies approximately 70% of the whole waste is now recovered for reuse. In the vehicle body coating process, a newly-developed robot arm type coating system is developed, which halves the coating waste. 2 refs., 9 figs., 1 tab.

  3. Trial to active seismic while drilling; Jinko shingen wo mochiita SWD eno kokoromi

    Energy Technology Data Exchange (ETDEWEB)

    Tsuru, T.; Kozawa, T. [Japan National Oil Corp., Tokyo (Japan). Technology Research Center

    1996-10-01

    This paper describes the development of a more stable SWD system with larger energy by adding an artificial seismic source near the bit. SWD is a technique by which the seismic wave generated while drilling of rocks by bit can be observed on the ground surface and the records equivalent to the reverse VSP can be obtained. For this system, a shell with a vibrator was fixed immediately on the bit as a sub-generator, and total energy of usual impact by the bit and vibration by the vibrator was used as a seismic source for SWD. For the seismic wave generation mechanism of this vibrator, the shell was resonated by the magnetostrictive element, and vibration was given to the bit and drilling pipe. When this seismic source is used, only single frequency is obtained for each vibration due to the utilization of resonance of shell. Therefore, the generation patterns should be made, by which wide band energy can be obtained after the interaction. Since the survey was conducted using this bottom hole seismic source at the drilling depth more than 3,000 m, it was necessary to enhance the vibration energy. 2 refs., 2 figs.

  4. Impacto del fenómeno ENOS en el régimen hidrometeorológico de Mendoza, Argentina

    Directory of Open Access Journals (Sweden)

    1998-01-01

    Full Text Available IMPACT DU PHÉNOMÈNE ENSO SUR LE RÉGIME HYDROMÉTÉOROLOGIQUE DE MENDONZA, ARGENTINE. Le principal objectif de ce travail est d’analyser les effets hydrométéorologiques causés par ENSO dans la province de Mendoza, Argentine. Ensuite on étudiera les conditions météorologiques prédominantes (situations synoptiques les plus fréquentes et les phénomènes de méso-échelle associés pendant l’hiver, en plaine et dans la cordillère. On caractérise la zone étudiée hydrologiquement et climatologiquement. On utilise pour cela les données de débits mensuels des principales rivières de la région ainsi que les données climatiques, synoptiques et des images de satellite. Notre étude couvre les événements ENSO de ce siècle et plus particulièrement ceux de 1982-1983, 1986-1987, 1991-1993 et les premières manifestations de celui de 1997-1998. Les principaux résultats montrent, pour les années ENSO, une forte augmentation des débits des rivières à la fin du printemps et pendant l’été provoqué par l’excès de précipitation de l’hiver antérieur. Les plus forts débits ont été observés au cours de l'événement chaud de 1982-1983, considéré jusqu'à 1997 comme le plus fort de ce siècle. L’impact principal sur le régime hydrique est dû à l’anomalie positive de précipitations dans la partie de la cordillère qui a un régime hivernal. On en conclut que les plus fortes précipitations de la cordillère sont reliées à une augmentation de la fréquence des passages de zones de basse pression et d’incursions d’onde longue à des latitudes plus basses que la normale. Ceci provoque une anomalie négative dans le champ de pression de surface et des couches moyennes de l’atmosphère. El objetivo principal del trabajo es analizar los efectos hidrometeorológicos que ocasiona el evento cálido ENOS en la provincia de Mendoza, Argentina. Los objetivos secundarios son considerar las condiciones meteorol

  5. Prospects to solar energy power generation in space. Uchu taiyo hatsuden eno tenbo

    Energy Technology Data Exchange (ETDEWEB)

    Kudo, I. (Electrotechnical Laboratory, Tsukuba (Japan))

    1993-05-01

    Solar energy power generation in space uses large arrays of solar cells developed on a geosynchronous orbit to obtain electric energy, which is transmitted to the earth using microwaves. The idea had already been advocated in 1968, which was followed a decade later by joint discussions done by NASA and DOE. The concept intended to take care of the U.S. power demand by using 60 power plant satellites, each having an output of 5 GW. This expanse of the scale, regarded reasonable even today, calls for the solar cell arrays in space spreading over an area of 10 km [times] 5 km if silicon solar cells with a conversion efficiency of 15% are used, and rectenna on the ground (a received wave converting facility) forming an ellipse of 10 km [times] 13 km (assuming a location at the north latitude of 36[degree]). Although there are a number of problems in the idea such as transportation means to lift construction materials into the space and effect of microwaves on the ionosphere and the ecosystems, the Agency of Industrial Science and Technology organized a 'committee for investigating and studying the space power generation systems' in the fiscal year 1991, and has been moving discussions forward since then. 7 refs., 5 figs.

  6. Sorption mechanism of solvent vapors to coals; Sekitan eno yobai joki no shuchaku kiko no kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Shimizu, K.; Takanohashi, T.; Iino, M. [Tohoku University, Sendai (Japan). Institute for Chemical Reaction Science

    1996-10-28

    With an objective to clarify the interactions between micropore structure of coal and solvent reagents, a sorption experiment was carried out under solvent saturated vapor pressure. Low-volatile bituminous coal, Pocahontas No. 3 coal, has the aromatic ring structure developed, and makes solvent more difficult to diffuse into coal, hence sorption amount is small. Methanol has permeated since its polarity is high. High-volatile bituminous coal, Illinois No. 6 coal, makes solvent penetrate easily, and the sorption amount was large with both of aromatic and polar solvents. Since brown coal, Beulah Zap coal, contains a large amount of oxygen, and hydrogen bonding is predominant, sorption amount of cyclohexane and benzene having no polarity is small. Methanol diffuses while releasing hydrogen bond due to its polarity, and its sorption amount is large. A double sorption model is available, which expresses the whole sorption amount as a sum of physical sorption amount and amount of permeation into coal. This model was applied when it explained successfully the sorption behavior of the solvents relative to coals, excepting some of the systems. However, also observed were such abnormal behavior as sorption impediment due to interactions between coal surface and solvents, and permeation impediment due to hydroxyl groups inside the coals. 1 ref., 10 figs., 2 tabs.

  7. Economics of Seed Yam Production Using Minisett Technique in Oyo State, Nigeria Economie de la production de semences d’igname à l’aide de la technique des minisetts dans l’Etat d’Oyo, au Nigéria Economía de la producción de semilla de ñame mediante la técnica «minisett» en el Estado de Oyo de Nigeria

    Directory of Open Access Journals (Sweden)

    Oguntade Adegboyega Eyitayo

    2011-01-01

    Full Text Available The study evaluated the economics of seed yam production using the minisett technique in Oyo State, South West Nigeria. The analysis was based on input and output data collected from sixty yam (Dioscorea spp. farmers and the existing market price in the area. The socio-economic characteristics of the respondents were analyzed using descriptive statistics. The structure of production costs was analyzed using percentages while gross margin analysis was used to determine the profitability of seed yam production using the minisett technique. Results of the analysis showed that seed yam production was dominated by men. The farmers had an average household size of 7.7 with a median of 7.0 and a mode of 7. Most of them were educated and had other occupations. The farmers assessed the technique as good, very good or excellent. The revenue per hectare of seed yam production was N337, 500. The total cost of production was N150,500 while the cost per seed yam was N16.72. On every naira invested in seed yam production using the minisett technique, farmers were expected to have N1.24 net returns. This shows that seed yam production using the minisett technique was a profitable venture in the study area. Labour costs alone accounted for 78.1% of the production cost. There is hence a need to deploy appropriate technologies to reduce these costs as a means of further reducing production costs and increasing the profitability of seed yams production using the minisett technique.L’étude portait sur l’évaluation de l’économie de la production de semences d’igname à l’aide de la technique des minisetts dans l’Etat d’Oyo, au sud-ouest du Nigéria. L’analyse était fondée sur les données d’entrée et de sortie recueillies auprès de soixante producteurs d’ignames (Dioscorea spp. et sur les prix de marché actuels dans la région. Les caractéristiques socio-économiques des personnes interrogées ont été analysées à l’aide de

  8. mRNA expression and localization of bNOS, eNOS and iNOS in human cervix at preterm and term labour

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    Byström Birgitta

    2005-08-01

    Full Text Available Abstract Background Preterm birth is the primary cause of the neonatal mortality and morbidity. There will be no preterm birth without a cervical softening. Nitric oxide (NO is shown to be a mediator of term cervical ripening. The aim of this study was to investigate mRNA expression of the three isomers of NO synthases (NOS and to identify them by immunohistochemistry in the human cervix at preterm birth compared to term. Methods The three isomers of NOS- inducible (iNOS, endothelial (eNOS and neuronal (bNOS – were investigated in the human cervix. The expression of mRNA was determined using Real-Time Multiplex RT-PCR. The localisation of synthases in the cervical tissue was analysed using immunohistochemistry. Cervical biopsies were obtained from 4 groups of women without clinical signs of infection: preterm (PTL, term labour (TL, preterm not in labour (PTnotL and term not in labour (TnotL patients. One-Way ANOVA, Kruskal-Wallis, Student t-test or Mann-Whitney test were applied as appropriate to determine statistically significant differences among the groups. Results Patients in preterm labour had significantly (p Conclusion The mRNA levels were higher in the preterm labour group compared to the women at term labour. The significant increase of the eNOS mRNA expression, from the unripe to the favourable cervical state during labour, may indicate a role of eNOS and supports the role of NO in the cervical ripening process. All the three synthases were identified by immunohistochemistry in all the groups of study.

  9. Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway

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    Fang Han

    2015-07-01

    Full Text Available Background: Induction of haemeoxygenase-1 (HO-1 increases adiponectin secretion by remodeling adipose tissue in obesity. The objective of our study is to explore whether HO-1 induction directly improves endothelial function independent of adiponectin changes in obese rats. Methods: Rats were divided into control and obesity groups. Aortic endothelial function was determined by measuring endothelium-dependent vasodilatation (EDV. Vascular segments of the obese rats were incubated in an organ bath in the presence or absence of cobalt protoporphyrin (CoPP or CoPP plus stannous protoporphyrin. Nitric oxide (NO production, superoxide anion production and NF-κB p65 expression in the aorta were determined. The expression of AMP-activated kinase (AMPK, Akt and endothelial nitric oxide synthase (eNOS in endothelial cells was determined by western blotting. The aortic rings from the obese rats were then incubated with CoPP in the presence of specific inhibitors of AMPK, phosphatidylinositol 3-kinase (PI3K or eNOS. Results: Acetylcholine-induced EDV was significantly attenuated in the obese rats, compared with the NC group (p p in vitro in the presence of inhibitors of AMPK, PI3K or eNOS. HO-1 induction with CoPP significantly increased the activation of the AMPK-PI3K/Akt-eNOS pathway and NO production in parallel with reduced superoxide anion production and NF-κB p65 expression in obese rats. Conclusions: HO-1 induction with CoPP directly improved endothelial function in obese rats independent of adiponectin changes. The mechanism of this protective effect is related to increasing NO production by activation of the AMPK-PI3K/Akt-eNOS signaling pathway.

  10. IL-8 and eNOS polymorphisms predict bevacizumab-based first line treatment outcomes in RAS mutant metastatic colorectal cancer patients.

    Science.gov (United States)

    Di Salvatore, Mariantonietta; Pietrantonio, Filippo; Orlandi, Armando; Del Re, Marzia; Berenato, Rosa; Rossi, Ernesto; Caporale, Marta; Guarino, Donatella; Martinetti, Antonia; Basso, Michele; Mennitto, Roberta; Santonocito, Concetta; Mennitto, Alessia; Schinzari, Giovanni; Bossi, Ilaria; Capoluongo, Ettore; Danesi, Romano; de Braud, Filippo; Barone, Carlo

    2017-03-07

    Predictive biomarkers of efficacy and toxicity of bevacizumab have not yet been validated. This study assessed the influence of IL-8, eNOS and VEGF-A polymorphisms in RAS mutated metastatic colorectal cancer patients receiving bevacizumab-based chemotherapy. 120 patients treated with first-line combination FOLFOX6 plus bevacizumab were included. A historical cohort of 112 RAS mutated colorectal cancer patients treated with FOLFOX6 alone served as control group. The following SNPs were analyzed: IL-8 c.-251T>A; eNOS c.-786T>C and c.-894G>T; VEGF-A c.936C>T, c.958T>C, c.1154A>G and c.2578C>A. Correlation of SNPs, baseline IL-8 serum levels and bevacizumab-efficacy was done. In the bevacizumab group, carriers of the IL-8 alleles c.-251TA+AA showed a shorter PFS (P=0.002) and OS (P=0.03) compared to TT alleles. Patients with pre-treatment IL-8 8.25 pg/ml showed significantly longer median PFS and OS (PFS: 10.9 vs 7.6 months, P=0.005; OS: 30.7 vs 18.2 months, P18,25 pg/ml). IL-8 c.-251TA+AA carriers had significantly higher IL-8 levels (PT was found associated with higher severe toxicity (P=0.0002) in patients carrying the c.-894TT genotype. Although our data need prospective validation, IL-8 and eNOS SNPs may be have a role as predictive biomarkers for bevacizumab efficacy and toxicity.

  11. Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS

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    Aldo Gerbino

    2011-07-01

    Full Text Available Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP, endothelial Nitric Oxide Synthase (eNOS and inducible Nitric Oxide Synthase (iNOS. The expression of these molecules in full-term human umbilical cord was investigated through immunohistochemistry and RT-PCR. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the human umbilical cord. mRNA expression for Oxytocin, ANP and eNOS suggest that these molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism locally regulating vascular tone. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 211–218

  12. Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS.

    Science.gov (United States)

    Mauro, Annamaria; Buscemi, Maria; Provenzano, Salvatore; Gerbino, Aldo

    2011-01-01

    Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton's jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. The aim of this study was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). The expression of these molecules in full-term human umbilical cord was investigated through immunohistochemistry and RT-PCR. Immunoreactivity for Orphanin FQ was detected in Wharton's jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton's jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the human umbilical cord. mRNA expression for Oxytocin, ANP and eNOS suggest that these molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism locally regulating vascular tone.

  13. Concentrations of eNOS, VEGF, ACE and PlGF in maternal blood as predictors of impaired fetal growth in pregnancy complicated by gestational hypertension/preeclampsia.

    Science.gov (United States)

    Wender-Ozegowska, Ewa; Zawiejska, Agnieszka; Iciek, Rafał; Brązert, Jacek

    2015-02-01

    To investigate into an association between circulating levels of vascular factors (VF: ACE, eNOS, PlGF and VEGF) and impaired fetal growth measured as a small for gestational age newborn (SGA) in women with gestational hypertension/preeclampsia. A prospective observational trial in 46 patients in singleton pregnancies. Concentrations of VF were compared between participants who delivered SGA versus non-SGA newborns. only low levels of ACE were associated with significantly increased risk for SGA (for a cut-off value, LR: 1.4-3.6). Circulating levels of VF are not sufficient predictors of SGA in pregnancies complicated by gestational hypertension/preeclampsia.

  14. Papel de eNOS, nNOS y NT-3 en patología cardiovascular y angiogénesis

    OpenAIRE

    Vicente Miralles, Diana

    2013-01-01

    El óxido nítrico es un mediador implicado en el desarrollo de la patología cardiovascular, la principal causa de mortalidad en nuestro entorno, pero su papel no ha sido completamente esclarecido. Recientemente, se ha establecido el mecanismo a través del cual actúa la NT-3 liberada en el endotelio vascular y se ha demostrado su capacidad para, a través de la activación de su receptor TrkC, fosforilar la eNOS y, como consecuencia, aumentar la síntesis de NO en las células que expresan este r...

  15. Influence of genetic variations in platelet glycoproteins and eNOS in the development of arterial ischaemia of lower limbs in type 2 diabetes mellitus patients.

    Science.gov (United States)

    Carvalhais, Virginia; Ruivães, Ema; Pina-Cabral, Luis Bernardo; Mesquita, Bárbara; Oliveira, Flávio; Monteiro, Maria Céu; Criado, Maria Begoña

    2016-12-01

    Endothelial and platelet dysfunction increase the atherothrombotic risk in diabetes mellitus patients. Therefore, arterial ischaemia of lower limbs is an important complication in diabetes mellitus. In the present work, type 2 diabetic patients were classified by a podiatrist into presence or absence of arterial ischaemia of lower limbs. Several polymorphisms in platelet glycoproteins and eNOS genes were evaluated. Our results suggest that the -5CC genotype in Kozak sequence of GPIbα may be associated with a higher risk of developing arterial ischaemia of lower limbs in type 2 diabetes mellitus patients. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. 3A.02: PREVALENCE OF HYPERTENSION AND ASSOCIATED FACTORS AMONG RESIDENTS OF IBADAN-NORTH LOCAL GOVERNMENT AREA OF OYO STATE, NIGERIA.

    Science.gov (United States)

    Sowemimo, I; Ajayi, I; Akpa, O; Ossai, E

    2015-06-01

    This study aimed to investigate the prevalence of hypertension and associated factors among the residents of Yemetu community; an urban-slum in Ibadan-North Local Government Area of Oyo State, Nigeria. A descriptive cross-sectional design was used. The study involved 806 respondents aged from 18-90 years from 171 households. They were selected by cluster sampling technique. It was a house-to-house survey. Behavioural risk factors were measured using World Health Organisation (WHO) STEPwise approach to chronic disease risk factor surveillance (STEPS 1 & 2), while physical activities were measured using International Physical Activity Questionnaire (IPAQ). Hypertension was defined according to WHO/International Society for Hypertension citeria (ISH). Data were analysed using descriptive statistics, Chi-square and binary logistic regression tests at p anti-hypertensive medication. Prior to the survey, 52.0% had checked their blood pressure within the past 12 months, 29.4% had checked more than a year ago, while 18.6% had never checked. The mean age of the respondents was 38.8 ± 15.6 years. The body mass index of the respondents was 5.2%, 52.0%, 29.5% and 13.3% for underweight, normal, overweight and obese, respectively. Alcohol and tobacco use were found in 11.5% and 3.2%, respectively. The result of binary logistic regression analysis revealed that hypertension was significantly associated with being in age groups 30-49 years (OR 2.258, 95% CI: 1.311 - 3.884), 50 years or more (OR 7.145, 95% CI: 3.644 - 14.011), and being overweight or obese (OR 2.281, 95% CI: 1.022 - 5.088). However, hypertension was inversely associated with being underweight (OR 0.537, 95% CI: 0.395 - 0.832). This study revealed a high prevalence of hypertension among the inhabitants of Yemetu community, which puts them at risk for cardiovascular disease. These data underscores the need for urgent steps to create awareness and implement interventions for prevention and early detection of

  17. Effects of a multi-level intervention on the pattern of physical activity among in-school adolescents in Oyo state Nigeria: a cluster randomised trial

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    Mojisola Morenike Oluwasanu

    2017-10-01

    Full Text Available Abstract Background Physical inactivity contributes to the global burden of non-communicable diseases. The pattern of physical activity in adulthood are often established during adolescence and sedentary behaviours in the early years could influence the development of diseases later in life. Studies on physical activity in Nigeria have focused largely on individual behaviours and the effects of school-based interventions have not been well investigated. The aim of the proposed study is to identify factors influencing; and evaluate the effects of a multi-level intervention on the physical activity behaviours of in-school adolescents in Oyo state, Nigeria. Methods The study will adopt a cluster randomised controlled trial design and schools will serve as the unit of randomisation. The sample size is 1000 in-school adolescents aged 10–19 years. The study will be guided by the socio-ecological model and theory of reasoned action and baseline data will be obtained through a mixed methods approach comprising a cross sectional survey to document the self-reported physical activity levels coupled with objectively measured physical activity levels using pedometers for a subset of the sample. Other measurements including weight, height, waist and hip circumferences, fitness level using the 20-m shuttle run test (20-mSRT and blood pressure will be obtained. The schools’ built environment and policy support for physical activity will be assessed using structured questionnaires coupled with key informant interviews and focus group discussions with the school authorities. Baseline findings will guide the design and implementation of a 12-week multi-level intervention. The primary outcome measures are self–reported and 7-day objectively measured physical activity. Other secondary outcome measures are body-mass-index for age, waist-to-hip ratio, cardioresiratory fitness and blood pressure. The association between behavioural factors and physical activity

  18. eNOS Glu298Asp polymorphism is associated with development of complicated plaques in patients from Serbia with advanced carotid atherosclerosis

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    Đurić Tamara

    2013-01-01

    Full Text Available Nitric oxide inhibits adhesion of thrombocytes, proliferation and migration of smooth muscle cells and restricts oxidation of atherogenic low-density lipoproteins. Therefore, decreased production or activity of NO may play a role in the initiation, progression or complications of atherosclerosis. The aim of this study was to estimate the effect of Glu298Asp eNOS gene polymorphism on the individual risk for development of complicated carotid atherosclerotic plaque in patients from Serbia with advanced carotid atherosclerosis (CA who had undergone endarterectomy. The study population included 233 patients. eNOS G894T gene polymorphism was identified by PCR and RFLP methods. Multivariate logistic regression analysis showed that Asp298Asp is an independent risk factor for the presence of complicated plaques in CA patients. Patients who were homozygous for the Asp298 allele had an adjusted OR of 4.36 for the development of complicated plaques compared to those that carry the Glu298 allele. Further validation and replication studies are needed. [Projekat Ministarstva nauke Republike Srbije, br. OI175085

  19. DFT, Hirshfeld surfaces, spectral and in vivo cytotoxic studies of 7a-Aza-B-homostigmast-5-eno [7a,7-d] tetrazole

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    Alam, Mahboob; Alam, Mohammad Jane; Nami, Shahab A. A.; Khan, Mohd Shoeb; Ahmad, Shabbir; Lee, Dong-Ung

    2015-11-01

    The DFT (B3LYP) calculations on a synthetic steroidal molecule 7a-Aza-B-homostigmast-5-eno [7a,7-d] tetrazole, C29H48N4, have been performed. The molecular structure, IR and NMR (13C and 1H) spectra of the present compound were interpreted using experiments (XRD, FTIR, NMR) as well as theoretical, B3LYP/6-311 + G(2d,p), calculations. The vibrational bands appearing in FTIR are assigned with great accuracy using animated modes. Molecular properties like HOMO-LUMO analysis, chemical reactivity descriptors, MEP mapping, dipole moment and Mullikan's atomic charges have been presented at the same level of theory. The theoretical results are found in good correlation with experimental data. Moreover, the Hirshfeld analysis was carried out to ascertain the secondary interactions and associated 2D fingerprint plots. The in vivo cytotoxicity of 7a-Aza-B-homostigmast-5-eno [7a,7-d] has also been carried out against brine shrimp nauplii by lethality bioassay.

  20. Effects of a pomegranate fruit extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesis.

    Science.gov (United States)

    de Nigris, Filomena; Williams-Ignarro, Sharon; Sica, Vincenzo; Lerman, Lilach O; D'Armiento, Francesco P; Byrns, Russell E; Casamassimi, Amelia; Carpentiero, Daniela; Schiano, Concetta; Sumi, Daigo; Fiorito, Carmela; Ignarro, Louis J; Napoli, Claudio

    2007-01-15

    Atherosclerosis is enhanced in arterial segments exposed to disturbed flow. Perturbed shear stress increases the expression of oxidation-sensitive responsive genes (such as ELK-1 and p-CREB). Polyphenolic antioxidants contained in the juice derived from the pomegranate contribute to the reduction of oxidative stress and atherogenesis during disturbed shear stress. To evaluate the effects of intervention with the Pomegranate Fruit Extract (PFE) rich in polyphones (punicalagin, which is a potent antioxidant) on ELK-1, p-CREB, and endothelial nitric oxide synthase (eNOS) expression induced by high shear stress in vitro and in vivo. At the doses used in the study, both the PFE and the regular pomegranate juice concentrate reduced the activation of ELK-1 and p-CREB and increased eNOS expression (which was decreased by perturbed shear stress) in cultured human endothelial cells and in atherosclerosis-prone areas of hypercholesterolemic mice. PFE and pomegranate juice increased cyclic GMP levels while there was no significant effect of both compounds on the conversion of L-arginine to L-citrulline. Administration of these compounds to hypercholesterolemic mice significantly reduced the progression of atherosclerosis and isoprostane levels and increased nitrates. This protective effect was relevant with PFE. Vasomotor reactivity was improved and EC(25) values in response to Ach and NONOate were significantly increased in treated mice in comparison to controls. This study indicates that the proatherogenic effects induced by perturbed shear stress can be also reversed by chronic administration of PFE.

  1. The coagulation factor V Leiden, MTHFRC677T variant and eNOS 4ab polymorphism in young Chinese population with ischemic stroke.

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    Shi, Congning; Kang, Xixiong; Wang, Yajie; Zhou, Yali

    2008-10-01

    The mechanisms of ischemic stroke in young adults are poorly understood. The main goal of the current investigation was to determine the possible contribution of genes affecting hemostasis, homocysteine metabolism and endothelial function on the occurrence of ischemic stroke in a cohort of young cases and corresponding controls. We evaluated in 97 consecutive patients referred to our center between April 2006 and July 2007 for a history of young adult ischemic stroke (age at first event, V Leiden, 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T and endothelial nitric oxide synthase (eNOS) 4ab gene variants. Ninety-nine subjects age and gender matched (18 to 45 y) from other departments of the Tiantan Hospital served as controls. Homozygosity for the TT genotype of the MTHFR gene was 29 of 97 (29.9%) in patients and 34 of 99 (34.3%) in controls; this difference was not statistically significant (p>0.05, chi2). Variation of factor V Leiden was not detected both in patients and in controls of this cohort. Genotype eNOS 4bb was 90 of 97 (92.8%) in patients and 81 of 99 (81.8%) in controls; this difference was statistically significant (pyoung Chinese population.

  2. Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy.

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    Alpcan, Serhan; Başar, Halil; Aydos, Tolga Reşat; Kul, Oğuz; Kısa, Üçler; Başar, Murad Mehmet

    2014-12-01

    We aimed to investigate the changes in endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expression and apoptotic index in rat testicular tissue, as well as serum and seminal plasma sex hormone levels after vasectomy, and the effect of ozone therapy (OT). Adult male Wistar rats were used (n=6 per group). Control (G1), sham for 4 weeks (G2) or 6 weeks (G3), orchiectomy at the 4(th) (G4) or 6(th) (G5) week after left vasectomy, orchiectomy at the 4(th) (G6) or 6(th) (G7) week after bilateral vasectomy, orchiectomy after 6 weeks OT following left (G8) or bilateral (G9) vasectomy, orchiectomy after 6 weeks OT (G10). In the left testes, while there were increases in eNOS and iNOS immunoreactivity and apoptotic indexes in G4 and G5, no changes were observed in contralateral testis. These values increased in G6 and G7, while OT inhibited these parameters in the left testis of G8 and both testes of G9. Sex hormone levels did not show any changes after vasectomy and ozone therapy. While OT was found to be protective against some parameters mentioned above under stress conditions, it seemed to cause some harmful effects when used in healthy conditions.

  3. Relationship of the MTHFD1 (rs2236225), eNOS (rs1799983), CBS (rs2850144) and ACE (rs4343) gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects.

    Science.gov (United States)

    Khatami, Mehri; Ratki, Farzaneh Morteza; Tajfar, Saba; Akrami, Fatemeh

    2017-09-01

    Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs) in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case-control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively). The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD. Copyright © 2017. Published by Elsevier Taiwan.

  4. Relationship of the MTHFD1 (rs2236225, eNOS (rs1799983, CBS (rs2850144 and ACE (rs4343 gene polymorphisms in a population of Iranian pediatric patients with congenital heart defects

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    Mehri Khatami

    2017-09-01

    Full Text Available Congenital heart defects are structural cardiovascular malformations that arise from abnormal formation of the heart or major blood vessels during the fetal period. To investigate the association of 4 single nucleotide polymorphisms (SNPs in the MTHFD1, eNOS, CBS and ACE genes, we evaluated their relationship with CHD in Iranian patients. In this case–control study, a total of 102 children with CHD and 98 control children were enrolled. Four SNPs including MTHFD1 G1958A, eNOS G894T, CBS C-4673G and ACE A2350G were genotyped by PCR-SSCP, Multiplex ARMS PCR and PCR-RFLP methods and confirmed by direct sequencing. We genotyped 102 patients and 98 controls for four polymorphisms by statistically analysis. There were three SNPs including MTHFD1 G1958A, eNOS G894T and ACE A2350G which might increase the risk of CHD, but CBS C-4673G was not significantly different between patients and controls. (P = 0.017, P = 0.048, P = 0.025 and P = 0.081 respectively. The allele frequencies of three SNPs for MTHFD1 G1958A, eNOS G894T and ACE A2350G in CHD are higher than that in control. Our results show that there is a significant relationship between MTHFD1 G1958A, eNOS G894T and ACE A2350G polymorphisms with CHD. Therefore, The AA and GA genotypes of MTHFD1 G1958A, TT and GT genotypes of eNOS G894T and the AA and GA genotypes of ACE A2350G are susceptible factors for CHD and may increase the risk of CHD.

  5. Association analysis of genetic variations of eNOS and α2ß1 integrin genes with type 2 diabetic retinopathy

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    Azmy R

    2012-07-01

    Full Text Available Rania Azmy,1 Ashraf Dawood,1 Ayman Kilany,1 Yasser El-Ghobashy,1 Amin Faisal Ellakwa,2 May El-Daly31Medical Biochemistry Department, 2Ophthalmology Department, Faculty of Medicine, 3National Liver Institute, Menoufiya University, Shebin Elkom, EgyptBackground: Diabetic retinopathy (DR is classically defined as a microvasculopathy that primarily affects the small blood vessels of the inner retina as a complication of diabetes mellitus. It has been suggested that nitric oxide (NO and α2β1 integrin (a platelet receptor for collagen play an important role in the pathogenesis of microvascular complications in DR.Aim: The aim of this study was to investigate the association of two candidate genes involved in the regulation of retinal vasculature, endothelial nitric oxide synthase (eNOS and α2β1 integrin (ITGA2 genes, with the development of DR in Egyptian patients with type 2 diabetes mellitus and to investigate whether genetic variants will affect the type of retinopathy (proliferative or nonproliferative.Methods: In this study, 70 patients were enrolled and categorized into two groups: (1 a DR group consisting of 50 patients with DR, which was further subclassified into 25 patients with nonproliferative DR (NPDR group and 25 patients with proliferative DR (PDR group and (2 a diabetes without retinopathy (DWR group, comprising 20 patients with type 2 diabetes of more than 10 years' duration who showed no signs of DR. Associations of the genetic polymorphisms of eNOS (G894T and ITGA2 (BgI II were studied. Polymerase chain reaction-restriction fragment length polymorphism analysis was performed for all samples to evaluate the genotypes and correlate with the phenotype of the disease.Results: The allele frequencies of both polymorphisms showed considerable differences between patients with and without DR. The GG genotype of G894T polymorphism of eNOS was associated with a 9.75-fold increased risk of DR (95% confidence interval 1.7–55.4 and the

  6. Redox-sensitive up-regulation of eNOS by purple grape juice in endothelial cells: role of PI3-kinase/Akt, p38 MAPK, JNK, FoxO1 and FoxO3a.

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    Mahmoud Alhosin

    Full Text Available The vascular protective effect of grape-derived polyphenols has been attributable, in part, to their direct action on blood vessels by stimulating the endothelial formation of nitric oxide (NO. The aim of the present study was to determine whether Concord grape juice (CGJ, which contains high levels of polyphenols, stimulates the expression of endothelial NO synthase (eNOS in porcine coronary artery endothelial cells and, if so, to determine the signaling pathway involved. CGJ dose- and time-dependently increased eNOS mRNA and protein levels and this effect is associated with an increased formation of NO in endothelial cells. The stimulatory effect of CGJ on eNOS mRNA is not associated with an increased eNOS mRNA stability and inhibited by antioxidants such as MnTMPyP, PEG-catalase, and catalase, and by wortmannin (an inhibitor of PI3-kinase, SB 203580 (an inhibitor of p38 MAPK, and SP 600125 (an inhibitor of JNK. Moreover, CGJ induced the formation of reactive oxygen species (ROS in endothelial cells and this effect is inhibited by MnTMPyP, PEG-catalase, and catalase. The CGJ-induced the phosphorylation of p38 MAPK and JNK kinases is abolished by MnTMPyP. CGJ induced phosphorylation of transcription factors FoxO1 and FoxO3a, which regulate negatively eNOS expression, and this effect is prevented by MnTMPyP, PEG-catalase, wortmannin, SB203580 and SP600125. Moreover, chromatin immunoprecipitation assay indicated that the FoxO3a protein is associated with the eNOS promoter in control cells and that CGJ induced its dissociation. Thus, the present study indicates that CGJ up-regulates the expression of eNOS mRNA and protein leading to an increased formation of NO in endothelial cells. The stimulatory effect of CGJ is a redox-sensitive event involving PI3-kinase/Akt, p38 MAPK and JNK pathways, and the inactivation of the FoxO transcription factors, FoxO1 and FoxO3a, thereby preventing their repression of the eNOS gene.

  7. L-Arginine ameliorates cardiac left ventricular oxidative stress by upregulating eNOS and Nrf2 target genes in alloxan-induced hyperglycemic rats

    Energy Technology Data Exchange (ETDEWEB)

    Ramprasath, Tharmarajan; Hamenth Kumar, Palani; Syed Mohamed Puhari, Shanavas; Senthil Murugan, Ponniah; Vasudevan, Varadaraj [Molecular Cardiology Unit, Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, Tamilnadu (India); Selvam, Govindan Sadasivam, E-mail: drselvamgsbiochem@rediffmail.com [Molecular Cardiology Unit, Department of Biochemistry, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, Tamilnadu (India)

    2012-11-23

    Highlights: Black-Right-Pointing-Pointer L-Arginine treatment reduced the metabolic disturbances in diabetic animals. Black-Right-Pointing-Pointer Antioxidant marker proteins were found high in myocardium by L-arginine treatment. Black-Right-Pointing-Pointer Elevated antioxidant status, mediates the reduced TBA-reactivity in left ventricle. Black-Right-Pointing-Pointer L-Arginine treatment enhanced the Nrf2 and eNOS signaling in left ventricle. Black-Right-Pointing-Pointer Improved cell survival signaling by arginine, offers a novel tactic for targeting. -- Abstract: Hyperglycemia is independently related with excessive morbidity and mortality in cardiovascular disorders. L-Arginine-nitric oxide (NO) pathway and the involvement of NO in modulating nuclear factor-E2-related factor-2 (Nrf2) signaling were well established. In the present study we investigated, whether L-arginine supplementation would improve the myocardial antioxidant defense under hyperglycemia through activation of Nrf2 signaling. Diabetes was induced by alloxan monohydrate (90 mg kg{sup -1} body weight) in rats. Both non-diabetic and diabetic group of rats were divided into three subgroups and they were administered either with L-arginine (2.25%) or L-NAME (0.01%) in drinking water for 12 days. Results showed that L-arginine treatment reduced the metabolic disturbances in diabetic rats. Antioxidant enzymes and glutathione levels were found to be increased in heart left ventricles, thereby reduction of lipid peroxidation by L-arginine treatment. Heart histopathological analysis further validates the reversal of typical diabetic characteristics consisting of alterations in myofibers and myofibrillary degeneration. qRT-PCR studies revealed that L-arginine treatment upregulated the transcription of Akt and downregulated NF-{kappa}B. Notably, transcription of eNOS and Nrf2 target genes was also upregulated, which were accompanied by enhanced expression of Nrf2 in left ventricular tissue from diabetic

  8. Abeeb olufemi Salaam Community Reintegration Oyo, Oyo State ...

    African Journals Online (AJOL)

    orientation; some parents may decide to enroll their children in Islamic education, called alm- ajiri (Abdulmalik, Omigbodun, Beida, & Ad- edokun, 2009; Salaam, 2011). Almajiri is a cor- rupt spelling of the Arabic word, al-muhajirin, which describes someone who leaves home in search of knowledge or for the sake of ...

  9. Abeeb olufemi Salaam Community Reintegration Oyo, Oyo State ...

    African Journals Online (AJOL)

    senegalensis), lemon grass (Cymbopogon citrates), mint plant (Mentha palustris), and garlic (Allium sativum); Kola nut is a caffeine-containing nut chewed in many West African cultures; Madran sukudie is a chemical used in preserving biological specimens, and embalming corpses; Dan kamaru is a stimulant root of a bitter ...

  10. Abeeb olufemi Salaam Community Reintegration Oyo, Oyo State ...

    African Journals Online (AJOL)

    rophynol, codeine, and alcohol misuse appear to exist among this group, in addition to other im- provised local drugs. ... ajiri (Abdulmalik, Omigbodun, Beida, & Ad- edokun, 2009 ..... its effect on actual cause and effects connec- tion, the ...

  11. eNOS se correlaciona com a biogênese mitocondrial em corações com cardiopatia congênita e cianose

    Directory of Open Access Journals (Sweden)

    Juan Xiao

    2012-09-01

    Full Text Available FUNDAMENTO: O programa de biogênese mitocondrial no coração parece apresentar remodelação adaptativa após estresse biomecânico e oxidativo. Os mecanismos adaptativos que protegem o metabolismo do miocárdio durante a hipóxia são coordenados, em parte, pelo óxido nítrico (NO. OBJETIVO: Observar a biogênese mitocondrial e expressão do óxido nítrico sintase (NOS em corações de cardiopatia congênita com cianose; discutir a resposta mitocondrial à hipóxia crônica do miocárdio. MÉTODOS: Foram investigados 20 pacientes com defeitos cardíacos cianóticos (n = 10 ou acianóticos (n = 10. Foram estudadas amostras do miocárdio na via de saída ventricular direita, tomadas durante a operação. A análise morfométrica de mitocôndrias foi realizada por microscopia eletrônica de transmissão. A relação mtDNA/nDNA foi determinada com PCR em tempo real. Os níveis de transcrição da subunidade I da citocromo c oxidase (COXI, coativador-1α do receptor γ ativado por proliferador de peroxissoma (PGC-1α, o fator respiratório nuclear 1 (NRF1, e fator de transcrição mitocondrial A (Tfam foram detectados por reação em cadeia da polimerase via transcriptase reversa (RT-PCR ativado por fluorescência em tempo real. Os níveis proteicos de COXI e nNOS, iNOS e eNOS foram medidos por técnica de Western Blot. RESULTADOS: A densidade volumétrica mitocondrial (Vv e a densidade numérica (Nv foram significativamente elevadas em pacientes com cianose, em comparação com a cardiopatia congênita acianótica. MtDNA elevada e suprarregulação dos níveis de COXI, PGC-1 α, NRF1 e Tfam mRNA foram observadas em pacientes cianóticos. Os níveis de proteína de COXI e eNOS foram significativamente maiores no miocárdio de pacientes cianóticos que nos de acianóticos. Os níveis de transcrição do PGC-1α se correlacionam com os níveis de eNOS. CONCLUSÃO: A biogênese mitocondrial é ativada no miocárdio da via de saída ventricular na

  12. [Effects of aerobic exercise training on antihypertension and expressions of VEGF, eNOS of skeletal muscle in spontaneous hypertensive rats].

    Science.gov (United States)

    Ma, Zhi-Yong; Zhao, Yong-Cai

    2014-07-01

    To investigate effects of exercise training on vascular regulators and discuss its antihypertensive mechanism. Rats were divided into three groups (n = 7): spontaneous hypertensive rats control group (SHR-C), training group (SHR-T) and normotensive wistar-kyoto control group (WKY-C). Aerobic exercise consisted of 10 weeks of swimming training for 5 days/week. Exercise duration was 40 min in the first week, then 50 min in the second week, from the third week to the end of training, duration was maintained at 60 min. After training, vascular endothelial growth factor (VEGF) and other biomarkers in soleus were measured by RT-PCR and immunoblotting. VEGF and endothelial NO synthase (eNOS) in SHR-C were lower than that in WKY-C (P antihypertensive effects.

  13. Passive leg movement enhances interstitial VEGF protein, endothelial cell proliferation, and eNOS mRNA content in human skeletal muscle

    DEFF Research Database (Denmark)

    Hellsten, Ylva; Rufener, Nora; Nielsen, Jens J

    2008-01-01

    to cultured endothelial cells revealed that dialysate obtained during leg movement induced a 3.2-fold higher proliferation rate (P enhanced (P RNA level fourfold above resting levels. VEGF mRNA and MMP-2 mRNA levels were...... unaffected. The results show that a session of passive leg movement, elevating blood flow and causing passive stretch, augments the interstitial concentrations of VEGF, the proliferative effect of interstitial fluid, and eNOS mRNA content in muscle tissue. We propose that enhanced blood flow and passive......The present study used passive limb movement as an experimental model to study the effect of increased blood flow and passive stretch, without enhanced metabolic demand, in young healthy male subjects. The model used was 90 min of passive movement of the leg leading to a 2.8-fold increase (P

  14. enos nízkofrekvenčního signálu po síti Ethernet v LabVIEW

    OpenAIRE

    Khait, Ranny

    2009-01-01

    Hlavním tématem předkládané práce je přenos nízkofrekvenčního signálu po síti Ethernet v LabVIEW. Práce obsahuje teoretický úvod, ve kterém jsou popsány typy přenosových protokolů, metody komprese, Ethernet, popis programu LabVIEW a jeho použití a biosignály. Praktická část je zaměřená na realizaci odesílání a přijmu dat po síti Ethernet. The main topic of presented thesis is LabVIEW based audio signal transmision via Ethernet. This thesis contains theoretical part, in which are described ...

  15. Role of C677T and A1298C MTHFR, A2756G MTR and -786 C/T eNOS gene polymorphisms in atrial fibrillation susceptibility.

    Directory of Open Access Journals (Sweden)

    Betti Giusti

    Full Text Available BACKGROUND: Hyperhomocysteinemia has been suggested to play a role in the NonValvular Atrial Fibrillation (NVAF pathogenesis. Polymorphisms in genes coding for homocysteine (Hcy metabolism enzymes may be associated with hyperhomocysteinemia and NVAF. METHODOLOGIES: 456 NVAF patients and 912 matched controls were genotyped by an electronic microchip technology for C677T and A1298C MTHFR, A2756G MTR, and -786C/T eNOS gene polymorphisms. Hcy was determined by an immunoassay method. PRINCIPAL FINDINGS: The genotype distribution of the four polymorphisms as well as genotype combinations did not differ in patients and controls. Hcy was higher in patients than in controls (15.2, 95%CI 14.7-15.7 vs 11.3, 95%CI 11.0-11.6 micromol/L; p<0.0001. In both populations, a genotype-phenotype association (p<0.0001 between Hcy and C677T MTHFR polymorphism was observed; in controls a significant (p = 0.029 association between tHcy and -786C/T eNOS polymorphism was also observed. At the multivariate analysis the NVAF risk significantly increased in the upper quartiles of Hcy compared to the lowest: OR from 2.8 (1.68-4.54 95%CI in Q2 to 12.9 (7.96-21.06 95%CI in Q4. CONCLUSIONS: Our data demonstrated the four polymorphisms, although able, at least in part, to affect Hcy, were not associated with an increased risk of NVAF per se or in combination.

  16. Extract from Ribes nigrum leaves in vitro activates nitric oxide synthase (eNOS) and increases CD39 expression in human endothelial cells.

    Science.gov (United States)

    Luzak, Boguslawa; Boncler, Magdalena; Rywaniak, Joanna; Dudzinska, Dominika; Rozalski, Marek; Krajewska, Urszula; Balcerczak, Ewa; Podsedek, Anna; Redzynia, Malgorzata; Watala, Cezary

    2014-12-01

    The aim of the present study was to evaluate whether blackcurrant leaf extract (BLE) modulates endothelium antithrombotic function, namely increases the expression/activity of ADPase (CD39) and augments the production of nitric oxide in human umbilical vein endothelial cells (HUVEC). It was found that BLE with proanthocyanidins (60 % of the total polyphenol content) increased the CD39-positive endothelial cell fraction (up to 10 % for 2.5 μg/ml, and up to 33 % for 15 μg/ml, p < 0.05 or less) in a concentration-dependent manner, and enhanced endothelial nitric oxide synthase (eNOS) activation (T495 phosphorylation decreased by 31 ± 6 % for 2.5 μg/ml and 48 ± 6 % for 15 μg/ml; S1177 phosphorylation increased by 13 ± 3 % for 2.5 μg/ml and 18 ± 7 % for 15 μg/ml, compared to untreated cells, p < 0.05 or less). Additionally, incubation for 24 or 48 h with BLE at a lower range of polyphenol concentrations, significantly increased cell viability with a maximal effect at 2.5 μg/ml (viability increased by 24.8 ± 1.0 % for 24 h and by 32.5 ± 2.7 % for 48-h time incubation, p < 0.0001). The increased CD39 expression and the increased eNOS activation in HUVEC can be regarded as the beneficial markers of the improvement of antiplatelet action of endothelial cells. Unexpectedly, these assumptions were not confirmed in the experimental model of platelet-endothelial cell interactions. These observations lead to the conclusion that BLE may improve endothelial cell viability at low physiological concentrations without affecting the antiplatelet action of endothelium.

  17. Altered vasoreactivity in neonatal rats with pulmonary hypertension associated with bronchopulmonary dysplasia: Implication of both eNOS phosphorylation and calcium signaling.

    Directory of Open Access Journals (Sweden)

    Eric Dumas de la Roque

    Full Text Available Bronchopulmonary dysplasia (BPD consists of an arrest of pulmonary vascular and alveolar growth, with persistent hypoplasia of the pulmonary microvasculature and alveolar simplification. In 25 to 40% of the cases, BPD is complicated by pulmonary hypertension (BPD-PH that significantly increases the risk of morbidity. In vivo studies suggest that increased pulmonary vascular tone could contribute to late PH in BPD. Nevertheless, an alteration in vasoreactivity as well as the mechanisms involved remain to be confirmed. The purpose of this study was thus to assess changes in pulmonary vascular reactivity in a murine model of BPD-PH. Newborn Wistar rats were exposed to either room air (normoxia or 90% O2 (hyperoxia for 14 days. Exposure to hyperoxia induced the well-known features of BPD-PH such as elevated right ventricular systolic pressure, right ventricular hypertrophy, pulmonary vascular remodeling and decreased pulmonary vascular density. Intrapulmonary arteries from hyperoxic pups showed decreased endothelium-dependent relaxation to acetylcholine without any alteration of relaxation to the NO-donor sodium nitroprusside. This functional alteration was associated with a decrease of lung eNOS phosphorylation at the Ser1177 activating site. In pups exposed to hyperoxia, serotonin and phenylephrine induced exacerbated contractile responses of intrapulmonary arteries as well as intracellular calcium response in pulmonary arterial smooth muscle cells (PASMC. Moreover, the amplitude of the store-operated Ca2+ entry (SOCE, induced by store depletion using a SERCA inhibitor, was significantly greater in PASMC from hyperoxic pups. Altogether, hyperoxia-induced BPD-PH alters the pulmonary arterial reactivity, with effects on both endothelial and smooth muscle functions. Reduced activating eNOS phosphorylation and enhanced Ca2+ signaling likely account for alterations of pulmonary arterial reactivity.

  18. Altered vasoreactivity in neonatal rats with pulmonary hypertension associated with bronchopulmonary dysplasia: Implication of both eNOS phosphorylation and calcium signaling.

    Science.gov (United States)

    Dumas de la Roque, Eric; Smeralda, Gwladys; Quignard, Jean-François; Freund-Michel, Véronique; Courtois, Arnaud; Marthan, Roger; Muller, Bernard; Guibert, Christelle; Dubois, Mathilde

    2017-01-01

    Bronchopulmonary dysplasia (BPD) consists of an arrest of pulmonary vascular and alveolar growth, with persistent hypoplasia of the pulmonary microvasculature and alveolar simplification. In 25 to 40% of the cases, BPD is complicated by pulmonary hypertension (BPD-PH) that significantly increases the risk of morbidity. In vivo studies suggest that increased pulmonary vascular tone could contribute to late PH in BPD. Nevertheless, an alteration in vasoreactivity as well as the mechanisms involved remain to be confirmed. The purpose of this study was thus to assess changes in pulmonary vascular reactivity in a murine model of BPD-PH. Newborn Wistar rats were exposed to either room air (normoxia) or 90% O2 (hyperoxia) for 14 days. Exposure to hyperoxia induced the well-known features of BPD-PH such as elevated right ventricular systolic pressure, right ventricular hypertrophy, pulmonary vascular remodeling and decreased pulmonary vascular density. Intrapulmonary arteries from hyperoxic pups showed decreased endothelium-dependent relaxation to acetylcholine without any alteration of relaxation to the NO-donor sodium nitroprusside. This functional alteration was associated with a decrease of lung eNOS phosphorylation at the Ser1177 activating site. In pups exposed to hyperoxia, serotonin and phenylephrine induced exacerbated contractile responses of intrapulmonary arteries as well as intracellular calcium response in pulmonary arterial smooth muscle cells (PASMC). Moreover, the amplitude of the store-operated Ca2+ entry (SOCE), induced by store depletion using a SERCA inhibitor, was significantly greater in PASMC from hyperoxic pups. Altogether, hyperoxia-induced BPD-PH alters the pulmonary arterial reactivity, with effects on both endothelial and smooth muscle functions. Reduced activating eNOS phosphorylation and enhanced Ca2+ signaling likely account for alterations of pulmonary arterial reactivity.

  19. Catechins Blunt the Effects of oxLDL and its Primary Metabolite Phosphatidylcholine Hydroperoxide on Endothelial Dysfunction Through Inhibition of Oxidative Stress and Restoration of eNOS in Rats

    Directory of Open Access Journals (Sweden)

    Hao-Hsiang Chang

    2017-11-01

    Full Text Available Background/Aims: We explored the effects of catechins (decaffeinated green tea extracts containing (–-epicatechin, (–-epigallocatechin, (–-epicatechin gallate and (–-epigallocatechin gallate on atherosclerosis risk factors, oxidized low-density lipoprotein (oxLDL and its primary metabolite, phosphatidylcholine hydroperoxide (PCOOH induced oxidative injury in cultured endothelial cell line and rats. Methods: We used endothelial cell line and male Wistar rats to determine the effect of catechins on oxLDL or PCOOH induced oxidative injury including apoptosis, H2O2 level, vascular responses and urinary 8-isoprostane and nitrite/nitrate concentration. Plasma catechins concentration was determined by a CoulArray HPLC. Responses of aortic and renal vasoconstriction were evaluated by a transonic meter and a full-field laser perfusion imager. Results: PCOOH administration significantly increased H2O2 amounts and cell apoptosis and decreased endothelial nitric oxide synthase (eNOS expression in the cultured endothelial cells. Catechins pretreatment significantly reduced PCOOH-elevated H2O2 amounts, endothelial cell apoptosis and partly recovered eNOS expression. Intravenous administration of oxLDL, PCOOH or H2O2, not native LDL, significantly decreased renal and aortic blood flow associated with enhanced ICAM-1 expression and 4-hydroxynoneal (4-HNE accumulation, and decreased eNOS expression in the male Wistrar rats. One hour after oral intake of green tea extracts, 4 peaks of catechins were found in the rat plasma. The increased plasma catechins significantly inhibited oxLDL-, PCOOH- or H2O2-induced renal and aortic vasoconstriction, decreased urinary 8-isoprostane levels, renal ICAM-1 expression and 4-HNE accumulation, and restored nitrite/nitrate amounts and eNOS activity. Conclusions: Our data suggests that catechins pretreatment decrease PCOOH-induced endothelial apoptosis and arterial vasoconstriction through the action of H2O2 inhibition

  20. Intracellular acidification reduces l-arginine transport via system y+L but not via system y+/CATs and nitric oxide synthase activity in human umbilical vein endothelial cells.

    Science.gov (United States)

    Ramírez, Marco A; Morales, Jorge; Cornejo, Marcelo; Blanco, Elias H; Mancilla-Sierpe, Edgardo; Toledo, Fernando; Beltrán, Ana R; Sobrevia, Luis

    2018-02-02

    l-Arginine is taken up via the cationic amino acid transporters (system y + /CATs) and system y + L in human umbilical vein endothelial cells (HUVECs). l-Arginine is the substrate for endothelial NO synthase (eNOS) which is activated by intracellular alkalization, but nothing is known regarding modulation of system y + /CATs and system y + L activity, and eNOS activity by the pHi in HUVECs. We studied whether an acidic pHi modulates l-arginine transport and eNOS activity in HUVECs. Cells loaded with a pH-sensitive probe were subjected to 0.1-20 mmol/L NH 4 Cl pulse assay to generate pHi 7.13-6.55. Before pHi started to recover, l-arginine transport (0-20 or 0-1000 μmol/L, 10 s, 37 °C) in the absence or presence of 200 μmol/L N-ethylmaleimide (NEM) (system y + /CATs inhibitor) or 2 mmol/L l-leucine (systemy + L substrate) was measured. Protein abundance for eNOS and serine 1177 or threonine 495 phosphorylated eNOS was determined. The results show that intracellular acidification reduced system y + L but not system y + /CATs mediated l-arginine maximal transport capacity due to reduced maximal velocity. Acidic pHi reduced NO synthesis and eNOS serine 1177 phosphorylation. Thus, system y + L activity is downregulated by an acidic pHi, a phenomenon that may result in reduced NO synthesis in HUVECs. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. Influencia de ENOS sobre las variaciones interanuales de ciertos ríos en América del Sur

    Directory of Open Access Journals (Sweden)

    1998-01-01

    plusieurs influences qui affectent la partie centrale des Andes tropicales soumise à l’influence du Pacifique équatorial, du bassin amazonien, de l’Océan Atlantique et de la Mer des Caraïbes. Las diferentes respuestas de los ríos sudamericanos a los estímulos de las fases de El Niño/Oscilación del Sur son estudiadas bajo tres condiciones: El Niño (EN, Anti-Niño (AN, y años normales. Ríos de la costa norte del Perú, Ecuador y el sur de Colombia experimentan altos caudales durante los veranos con EN y bajas descargas durante AN. Las descargas son normales en los otros años. Los ríos de los Andes peruanos y el Altiplano tienen bajos caudales durante EN y descargas normales en las otras dos condiciones. Los ríos de Chile central y los Andes de Argentina experimentan altos caudales durante EN y bajos durante AN. En Patagonia y el sur de Chile existe una independencia con respecto a las fases de ENOS ya que las descargas más altas tienen lugar durante años normales. Los ríos de la Pampa, las tierras bajas de la cuenca del Plata y el extremo sur de Brasil, tienen altas descargas durante EN y bajas durante AN. Los ríos de la costa central de Brasil demuestran independencia con respecto a las tres condiciones. En el noreste de Brasil las descargas son bajas durante EN y altas durante AN. La cuenca del Amazonas tiene diversas respuestas: en la sección central los ríos experimentan bajos caudales durante EN mientras que los tributarios de los Andes tienden a ofrecer altas descargas durante algunos EN. Los ríos de las Guayanas, de Venezuela y Colombia expuestos al Caribe experimentan bajos caudales durante EN y altos volúmenes durante AN. En los Andes del sur de Colombia y Ecuador existe una mezcla: algunos ríos tienen altos caudales durante EN extremadamente fuertes, otros durante las fases AN. Esta región sintetiza las distintas influencias que afectan la parte central de los Andes tropicales, sometida a influencias del Pacífico ecuatorial, de la cuenca

  2. Efeitos da atorvastatina e do polimorfismo T-786C do gene eNOS sobre parâmetros do metabolismo lipídico plasmático Effects of atorvastatin and T-786C polymorphism of eNOS gene on plasma metabolic lipid parameters

    Directory of Open Access Journals (Sweden)

    Vanessa Helena de Souza Zago

    2013-01-01

    Full Text Available FUNDAMENTO: A atividade do óxido nítrico sintase endotelial (eNOS pode ser modulada pelo colesterol da lipoproteína de alta densidade (HDL-C, estatinas ou polimorfismos, como o T-786C de eNOS. OBJETIVO: Este estudo teve como objetivo avaliar se o polimorfismo T-786C está associado a alterações nos efeitos da atorvastatina no perfil lipídico, nas concentrações de metabólitos de óxido nítrico (NO e da proteína C reativa de alta sensibilidade (PCR-as. MÉTODOS: Trinta voluntários do sexo masculino, assintomáticos, com idade entre 18-56 anos foram genotipados e classificados de acordo com a ausência (TT, n = 15 ou presença (CC, n = 15 do polimorfismo. Eles foram selecionados aleatoriamente para a utilização de placebo e atorvastatina (10 mg/dia por 14 dias. Após cada tratamento foram medidos lípides, lipoproteínas, frações HDL2 e HDL3, atividade da proteína de transferência de colesteril éster (CETP, metabólitos de NO e PCR-as. RESULTADOS: As comparações entre genótipos após a administração de placebo mostraram aumento da atividade da CETP polimorfismo-dependente (TT, 12 ± 7; CC, 22 ± 12, p BACKGROUND: Endothelial nitric oxide synthase (eNOS activity may be modulated by high-density lipoprotein cholesterol (HDL-C, statins or polymorphisms, such as the T-786C of eNOS. OBJECTIVE: This study aimed at evaluating if the T-786C polymorphism is associated with changes of atorvastatin effects on the lipid profile, on the concentrations of metabolites of nitric oxide (NO and of high sensitivity C-reactive protein (hsCRP. METHODS: Thirty male volunteers, asymptomatic, aged between 18 and 56 years were genotyped and classified according to absence (TT, n = 15 or presence (CC, n = 15 of the polymorphism. They were randomly selected for the use of placebo or atorvastatin (10 mg/day/14 days. After each treatment lipids, lipoproteins, HDL2 and HDL3 composition, cholesteryl ester transfer protein (CETP activity, metabolites of NO

  3. Efeitos da atorvastatina e do polimorfismo T-786C do gene eNOS sobre parâmetros do metabolismo lipídico plasmático Effects of atorvastatin and T-786C polymorphism of eNOS gene on plasma metabolic lipid parameters

    Directory of Open Access Journals (Sweden)

    Vanessa Helena de Souza Zago

    2012-01-01

    Full Text Available FUNDAMENTO: A atividade do óxido nítrico sintase endotelial (eNOS pode ser modulada pelo colesterol da lipoproteína de alta densidade (HDL-C, estatinas ou polimorfismos, como o T-786C de eNOS. OBJETIVO: Este estudo teve como objetivo avaliar se o polimorfismo T-786C está associado a alterações nos efeitos da atorvastatina no perfil lipídico, nas concentrações de metabólitos de óxido nítrico (NO e da proteína C reativa de alta sensibilidade (PCR-as. MÉTODOS: Trinta voluntários do sexo masculino, assintomáticos, com idade entre 18-56 anos foram genotipados e classificados de acordo com a ausência (TT, n = 15 ou presença (CC, n = 15 do polimorfismo. Eles foram selecionados aleatoriamente para a utilização de placebo e atorvastatina (10 mg/dia por 14 dias. Após cada tratamento foram medidos lípides, lipoproteínas, frações HDL2 e HDL3, atividade da proteína de transferência de colesteril éster (CETP, metabólitos de NO e PCR-as. RESULTADOS: As comparações entre genótipos após a administração de placebo mostraram aumento da atividade da CETP polimorfismo-dependente (TT, 12 ± 7; CC, 22 ± 12, p BACKGROUND: Endothelial nitric oxide synthase (eNOS activity may be modulated by high-density lipoprotein cholesterol (HDL-C, statins or polymorphisms, such as the T-786C of eNOS. OBJECTIVE: This study aimed at evaluating if the T-786C polymorphism is associated with changes of atorvastatin effects on the lipid profile, on the concentrations of metabolites of nitric oxide (NO and of high sensitivity C-reactive protein (hsCRP. METHODS: Thirty male volunteers, asymptomatic, aged between 18 and 56 years were genotyped and classified according to absence (TT, n = 15 or presence (CC, n = 15 of the polymorphism. They were randomly selected for the use of placebo or atorvastatin (10 mg/day/14 days. After each treatment lipids, lipoproteins, HDL2 and HDL3 composition, cholesteryl ester transfer protein (CETP activity, metabolites of NO

  4. Numerical methods for systems of conservation laws of mixed type using flux splitting

    Science.gov (United States)

    Shu, Chi-Wang

    1990-01-01

    The essentially non-oscillatory (ENO) finite difference scheme is applied to systems of conservation laws of mixed hyperbolic-elliptic type. A flux splitting, with the corresponding Jacobi matrices having real and positive/negative eigenvalues, is used. The hyperbolic ENO operator is applied separately. The scheme is numerically tested on the van der Waals equation in fluid dynamics. Convergence was observed with good resolution to weak solutions for various Riemann problems, which are then numerically checked to be admissible as the viscosity-capillarity limits. The interesting phenomena of the shrinking of elliptic regions if they are present in the initial conditions were also observed.

  5. Docosahexaenoic acid inhibits vascular endothelial growth factor (VEGF)-induced cell migration via the GPR120/PP2A/ERK1/2/eNOS signaling pathway in human umbilical vein endothelial cells.

    Science.gov (United States)

    Chao, Che-Yi; Lii, Chong-Kuei; Ye, Siou-Yu; Li, Chien-Chun; Lu, Chia-Yang; Lin, Ai-Hsuan; Liu, Kai-Li; Chen, Haw-Wen

    2014-05-07

    Cell migration plays an important role in angiogenesis and wound repair. Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is essential for endothelial cell survival, proliferation, and migration. Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid, shows both anti-inflammatory and antioxidant activities in vitro and in vivo. This study investigated the molecular mechanism by which DHA down-regulates VEGF-induced cell migration. HUVECs were used as the study model, and the MTT assay, Western blot, wound-healing assay, and phosphatase activity assay were used to explore the effects of DHA on cell migration. GPR120 is the putative receptor for DHA action. The results showed that DHA, PD98059 (an ERK1/2 inhibitor), and GW9508 (a GPR120 agonist) inhibited VEGF-induced cell migration. In contrast, pretreatment with okadaic acid (OA, a PP2A inhibitor) and S-nitroso-N-acetyl-DL-penicillamine (an NO donor) reversed the inhibition of cell migration by DHA. VEGF-induced cell migration was accompanied by phosphorylation of ERK1/2 and eNOS. Treatment of HUVECs with DHA increased PP2A enzyme activity and decreased VEGF-induced phosphorylation of ERK1/2 and eNOS. However, pretreatment with OA significantly decreased DHA-induced PP2A enzyme activity and reversed the DHA inhibition of VEGF-induced ERK1/2 and eNOS phosphorylation. These results suggest that stimulation of PP2A activity and inhibition of the VEGF-induced ERK1/2/eNOS signaling pathway may be involved in the DHA suppression of VEGF-induced cell migration. Thus, the effect of DHA on angiogenesis and wound repair is at least partly by virtue of its attenuation of cell migration.

  6. Association between the Polymorphism of Glu298Asp in Exon 7 of the eNOS Gene with Foot Ulcer and Oxidative Stress in Patients with Type 2 Diabetes.

    Science.gov (United States)

    Sadati, Seyedeh Maryam; Radfar, Mania; Hamidi, Armita Kakavand; Abdollahi, Mohammad; Qorbani, Mostafa; Esfahani, Ensieh Nasli; Amoli, Mahsa M

    2017-05-10

    Diabetic foot ulcer (DFU) is a common and major manifestation in patients with diabetes. Oxidative stress (OS) plays an important role in diabetic complications, such as DFU. Nitric oxide deficiency contributes to the impairment of diabetic wound healing. The aim of this study was to examine the association between the eNOS Glu298Asp polymorphism and DFU and oxidative stress in patients with type 2 diabetes mellitus in an Iranian population. In this case-control study, 123 patients with type 2 diabetes and DFU and 134 patients without DFU were recruited. The genotypes of eNOS Glu298Asp polymorphism in exon 7 were determined by the polymerase chain reaction-restriction fragment length polymorphism analysis. We measured the levels of thiobarbituric reactive substances and ferric-reducing ability of plasma as the potential markers of OS. There were significant differences in genotype frequencies of eNOS Glu298Asp polymorphism between case and control groups (GG+TG vs. TT; p=0.002; OR=0.22, 95% CI 0.83 to 0.62). Also, the frequency of the T allele in cases was less common than in controls (p=0.004). There was no significant difference in levels of OS parameters and various genotypes (p>0.05). These results imply that the T allele might be protective against DFU. Copyright © 2017 Diabetes Canada. Published by Elsevier Inc. All rights reserved.

  7. A combination of genistein and magnesium enhances the vasodilatory effect via an eNOS pathway and BK(Ca) current amplification.

    Science.gov (United States)

    Sun, Lina; Hou, Yunlong; Zhao, Tingting; Zhou, Shanshan; Wang, Xiaoran; Zhang, Liming; Yu, Guichun

    2015-04-01

    The phytoestrogen genistein (GST) and magnesium have been independently shown to regulate vascular tone; however, their individual vasodilatory effects are limited. The aim of this study was to examine the combined effects of GST plus magnesium on vascular tone in mesenteric arteries. The effects of pretreatment with GST (0-200 μmol/L), MgCl2 (0-4.8 mmol/L) and GST plus MgCl2 on 10 μmol/L phenylephrine (PE) precontracted mesenteric arteries in rats were assessed by measuring isometric force. BK(Ca) currents were detected by the patch clamp method. GST caused concentration- and partial endothelium-dependent relaxation. Magnesium resulted in dual adjustment of vascular tone. Magnesium-free solution eliminated the vasodilatation of GST in both endothelium-intact and denuded rings. GST (50 μmol/L) plus magnesium (4.8 mmol/L) caused stronger relaxation in both endothelium-intact and denuded rings. Pretreatment with the nitric oxide synthase (NOS) inhibitor L-N-nitroarginine methyl ester (L-NAME, 100 μmol/L) significantly inhibited the effects of GST, high magnesium, and the combination of GST and magnesium. BK(Ca) currents were amplified to a greater extent when GST (50 μmol/L) was combined with 4.8 versus 1.2 mmol/L Mg(2+). Our data suggest that GST plus magnesium provides enhanced vasodilatory effects in rat mesenteric arteries compared with that observed when either is used separately, which was related to an eNOS pathway and BK(Ca) current amplification.

  8. Research on application of heat pump on cyclamen culture. Shikuramen saibai eno heat pump tekiyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Nada, Hidetoshi (Kyushu Electric Power Co., Inc., Fukuoka (Japan))

    1989-03-30

    The culture test by introduction of heat pump was conducted for the culture process of cyclamen. As the result of the test, cultivating technology which was capable to deliver cyclamen twice a year, in September and December, together with the reliable technology to cultivate it in large pots (No 7, 8 flower pots) to deliver it at the end of year, were established. Disease and outbreak of injurious insects such as wilt and grey mold were capable to be almost prevented by air-conditioning and dehumidifying system, therefore superior quality cyclamen with beautiful flowers was grown. Further, under-bench culture test which has never been examined, was applied to Daimonji-so, then much interest supplemented by its running cost, was attained. Beside the above, the profitability of heat pump was verified, and energy conservation and labor saving were realized by the automation on opening and closing of green house windows and curtains, on starting and stopping of heat pump which was operated in compliance with set temperature, humidity and quantuty of solar radiation. 7 figs., 10 tabs.

  9. Application of permeable metal die for non-ferrous alloy casting; Tsukisei kanagata no keigokin kanagata chuzo eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Kato, T. [Shinto Kogyo Ltd., Nagoya (Japan)

    1997-05-25

    This paper introduces a permeable metal die developed to apply to casting non-ferrous alloys. Gas is discharged through micropores distributed uniformly on a die material. The die is classified into a die having pores with a diameter of 7 {mu}m used for die-casting, and another having pores with a diameter of 30 {mu}m used for gravity casting and low-pressure casting. Cr/Mo-based high-alloy special steel powder is mixed with high-alloy special steel short fibers of the same system for reinforcement to make a die material through compressive formation and sintering. The material undergoes cutting and grinding to fabricate a metal die. Its structure is martensite. Basic tests were given on the die to test heat check resisting performance, molten metal running performance, and mechanical properties of casts. In addition, discussions were given on effects of coating of die release agent and refractories on air permeability. The following effects were verified: inadequate molten metal run is improved; gas defects are eliminated; transcribability of die shape is enhanced; surface grade of casts is improved; and casting cycle time is shortened. Aluminum automotive parts and aluminum containers are mass-produced by using this metal. 4 refs., 16 figs., 3 tabs.

  10. Despite the lack of association between different genotypes and the presence of prostate cancer, endothelial nitric oxide synthase a/b (eNOS4a/b) polymorphism may be associated with advanced clinical stage and bone metastasis.

    Science.gov (United States)

    Sanli, Oner; Kucukgergin, Canan; Gokpinar, Murat; Tefik, Tzevat; Nane, Ismet; Seckin, Sule

    2011-01-01

    To investigate the relationship between the distribution of endothelial NO synthase (eNOS4a/b) gene polymorphism and clinical features of prostate cancer (PCa). One hundred thirty-two patients with PCa (mean age 64.10 ± 7.23 years) and 158 healthy controls (mean age 62.50 ± 7.53 years) with normal serum total prostate specific antigen (PSA) levels (bone metastasis (Group 2), and patients with bone metastasis (Group 3). Genotypes (aa, bb, ab) for eNOS4a/b gene polymorphisms were identified by polymerase chain reaction analysis. Meanwhile, plasma nitrate and nitrite levels (NO(x)) were used to estimate the amounts of endogenous NO formation for both groups of patients. Despite lack of statistically significant differences between PCa patients and the control group in terms of distribution of genotypes and frequency of alleles, plasma NO(x) levels were found to be significantly increased in PCa patients compared with controls. Meanwhile, there was no significant difference between the group of PCa patients with high and low grade tumors (Gleason score ≥ 7 vs. bone metastasis in comparison with bb genotype. Moreover, multivariable logistic regression analysis revealed that eNOS4a/b polymorphism and plasma NOx levels were predictive factors for developing bone metastasis and high stage disease after adjustment for age and BMI. Our data did not reveal any relationship between any of these genotypes and the presence of PCa. However, the finding that PCa patients with bb genotype generally manifest localized disease and develop bone metastasis less frequently in comparison patients with a-allele may indicate an important role for this polymorphism in the molecular pathophysiology of PCa. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. MicroRNA-27b plays a role in pulmonary arterial hypertension by modulating peroxisome proliferator-activated receptor γ dependent Hsp90-eNOS signaling and nitric oxide production

    Energy Technology Data Exchange (ETDEWEB)

    Bi, Rui; Bao, Chunrong; Jiang, Lianyong; Liu, Hao; Yang, Yang; Mei, Ju; Ding, Fangbao, E-mail: dbcar126@126.com

    2015-05-01

    Pulmonary artery endothelial dysfunction is associated with pulmonary arterial hypertension (PAH). Based on recent studies showing that microRNA (miR)-27b is aberrantly expressed in PAH, we hypothesized that miR-27b may contribute to pulmonary endothelial dysfunction and vascular remodeling in PAH. The effect of miR-27b on pulmonary endothelial dysfunction and the underlying mechanism were investigated in human pulmonary artery endothelial cells (HPAECs) in vitro and in a monocrotaline (MCT)-induced model of PAH in vivo. miR-27b expression was upregulated in MCT-induced PAH and inversely correlated with the levels of peroxisome proliferator-activated receptor (PPAR)-γ, and miR-27b inhibition attenuated MCT-induced endothelial dysfunction and remodeling and prevented PAH associated right ventricular hypertrophy and systolic pressure in rats. PPARγ was confirmed as a direct target of miR-27b in HPAECs and shown to mediate the effect of miR-27b on the disruption of endothelial nitric oxide synthase (eNOS) coupling to Hsp90 and the suppression of NO production associated with the PAH phenotype. We showed that miR-27b plays a role endothelial function and NO release and elucidated a potential mechanism by which miR-27b regulates Hsp90-eNOS and NO signaling by modulating PPARγ expression, providing potential therapeutic targets for the treatment of PAH. - Highlights: • miR-27b plays a role in endothelial function and NO release. • miR-27b inhibition ameliorates MCT-induced endothelial dysfunction and PAH. • miR-27b targets PPARγ in HPAECs. • miR-27b regulates PPARγ dependent Hsp90-eNOS and NO signaling.

  12. Genome-wide association study using a high-density SNP-array and case-control design identifies a novel essential hypertension susceptibility locus in the promoter region of eNOS

    Science.gov (United States)

    Salvi, Erika; Kutalik, Zoltán; Glorioso, Nicola; Benaglio, Paola; Frau, Francesca; Kuznetsova, Tatiana; Arima, Hisatomi; Hoggart, Clive; Tichet, Jean; Nikitin, Yury P.; Conti, Costanza; Seidlerova, Jitka; Tikhonoff, Valérie; Stolarz-Skrzypek, Katarzyna; Johnson, Toby; Devos, Nabila; Zagato, Laura; Guarrera, Simonetta; Zaninello, Roberta; Calabria, Andrea; Stancanelli, Benedetta; Troffa, Chiara; Thijs, Lutgarde; Rizzi, Federica; Simonova, Galina; Lupoli, Sara; Argiolas, Giuseppe; Braga, Daniele; D’Alessio, Maria C.; Ortu, Maria F.; Ricceri, Fulvio; Mercurio, Maurizio; Descombes, Patrick; Marconi, Maurizio; Chalmers, John; Harrap, Stephen; Filipovsky, Jan; Bochud, Murielle; Iacoviello, Licia; Ellis, Justine; Stanton, Alice V.; Laan, Maris; Padmanabhan, Sandosh; Dominiczak, Anna F.; Samani, Nilesh J.; Melander, Olle; Jeunemaitre, Xavier; Manunta, Paolo; Shabo, Amnon; Vineis, Paolo; Cappuccio, Francesco P.; Caulfield, Mark J.; Matullo, Giuseppe; Rivolta, Carlo; Munroe, Patricia B.; Barlassina, Cristina; Staessen, Jan A; Beckmann, Jacques S.; Cusi, Daniele

    2012-01-01

    Essential hypertension is a multi-factorial disorder and is the main risk factor for renal and cardiovascular complications. The research on the genetics of hypertension has been frustrated by the small predictive value of the discovered genetic variants. The HYPERGENES Project investigated associations between genetic variants and essential hypertension pursuing a two-stage study by recruiting cases and controls from extensively characterized cohorts recruited over many years in different European regions. The discovery phase consisted of 1,865 cases and 1,750 controls genotyped with 1M Illumina array. Best hits were followed up in a validation panel of 1,385 cases and 1,246 controls that were genotyped with a custom array of 14,055 markers. We identified a new hypertension susceptibility locus (rs3918226) in the promoter region of the endothelial nitric oxide synthase (eNOS) gene (odds ratio 1.54; 95% CI 1.37-1.73; combined p=2.58·10−13). A meta-analysis, using other in-silico/de novo genotyping data for a total of 21714 subjects, resulted in an overall odds ratio of 1.34 (95% CI 1.25-1.44, p=1.032·10−14). The quantitative analysis on a population-based sample revealed an effect size of 1.91 (95% CI 0.16-3.66) for systolic and 1.40 (95% CI 0.25-2.55) for diastolic blood pressure. We identified in-silico a potential binding site for ETS transcription-factors directly next to rs3918226, suggesting a potential modulation of eNOS expression. Biological evidence links eNOS with hypertension, as it is a critical mediator of cardiovascular homeostasis and blood pressure control via vascular tone regulation. This finding supports the hypothesis that there may be a causal genetic variation at this locus. PMID:22184326

  13. Morphology and endothelial function of microvessels in microvascular angina. With special reference to the expression of endothelial nitric oxide synthase (eNOS) before and after treatment with K{sub ATP} channel opener

    Energy Technology Data Exchange (ETDEWEB)

    Akao, Hironobu [Kanazawa Medical Univ., Uchinada, Ishikawa (Japan)

    2000-03-01

    In patients with microvascular angina (MVA), we studied myocardial metabolic disturbance, morphological characteristics of microvessels and the presence or absence of the expression of eNOS before and after treatment with K{sub ATP} channel opener. The study population consisted of 21 patients with MVA, and 8 patients with valvular disease and without ischemic lesions who served as the control. Myocardial metabolic disturbance was assessed by calculating the standardized uptake value (SUV) of {sup 18}FDG from glucose metabolism using nuclear imaging by {sup 18}FDG-PET, and quantitatively determining the severity of ischemia. Before treatment with K{sub ATP} channel opener, {sup 18}FDG uptake was detected in all 21 patients (100%) with MVA, by fasting {sup 18}FDG-PET during rest. After treatment, improvement at the site of uptake was detected in 19 of the 21 patients (90.5%). Before treatment, SUV of all 21 patients was 1.43{+-}0.76 and, after treatment, SUV was 0.39{+-}0.17; a statistically significant improvement (p<0.001). Histologically, the right ventricular myocardial specimens obtained by biopsy were studied for morphological changes under light and electron microscopes. In all MVA patients, histological examination revealed severe narrowing of the lumen with marked thickening of the media and swelling and proliferation of the endothelial cells in many arterioles, most of which also showed thickening of the basement membrane in all MVA patients. Many capillary vessels also showed the appearance of medial smooth muscle cells and swelling and proliferating of endothelial cells, resulting in narrowing of the lumen. This narrowing is the likely cause of ischemia. The eNOS expression in the arterioles and capillaries was immnohistochemically determined in 11 of the 21 patients. Before treatment, the eNOS expression was barely observable. After treatment, it was increased markedly to a level similar to that of the controls. In conclusion, the eNOS expression in the

  14. Puruvana puruvanavana : [luuletused] / Eno Raud

    Index Scriptorium Estoniae

    Raud, Eno, 1928-1996

    2000-01-01

    Sisu: Puruvana puruvanavana ; Vetesell ; Pada ja katel ; Nääripäkapikk ; Vanapaar ; Mere peremees ; Lepamaim ; Kiilaspää kiilasjääl ; Tõugu unistus ; Naljatilk silk ; Muttide jalutuskäik ; Hiir lumes ; Kalakari salakaril ; Naljalammas ; Mesilastest ; Kure mure ; Voki laul karule ; Põrsalaul ; Kurk ja kõrvits ; Tuhk ja tina ; Õige pea ja vale pea ; Suu pea, puupea! ; Siga õuel

  15. Silicic acid in drinking water prevents age-related alterations in the endothelium-dependent vascular relaxation modulating eNOS and AQP1 expression in experimental mice: an immunohistochemical study.

    Science.gov (United States)

    Buffoli, Barbara; Foglio, Eleonora; Borsani, Elisa; Exley, Christopher; Rezzani, Rita; Rodella, Luigi Fabrizio

    2013-06-01

    The maintenance of endothelial integrity is of great importance in coping with age-related vascular alterations. Endothelium-derived nitric oxide is one of the various vasoactive substances able to regulate vascular tone and homeostasis, and whose decrease is known to be related with senescence in endothelial cells. There are reports on the efficacy of silicon, especially as silicic acid, in protecting vascular integrity during age-related vascular diseases. The aim of this study was to evaluate the ability of supplementation of silicic acid in drinking water in the maintenance of vascular health in a mouse model of early physiological aging. In particular, we evaluated the relationship between Si supplementation and endothelial nitric oxide synthase (eNOS) expression, taking into account also the aquaporin-1 (AQP-1) isoform that, as recently reported, seems to be involved in nitric oxide transport across cell membranes. Our results showed that silicic acid supplementation increased both eNOS and AQP-1 expression, suggesting that silicic acid modulation of endothelial nitric oxide synthase and aquaporin-1 could represent a potential strategy against age-related vascular senescence. Copyright © 2012 Elsevier GmbH. All rights reserved.

  16. Climatologia e variabilidade sazonal do número de ondas de calor e de frio no Rio Grande do Sul associadas ao ENOS Climatology and seasonal variability of the number of warm and cold waves in Rio Grande do Sul associated to ENOS

    Directory of Open Access Journals (Sweden)

    Mári Ândrea Feldman Firpo

    2012-03-01

    Full Text Available Este trabalho investiga a variabilidade sazonal das ocorrências de ondas de frio e calor no Rio Grande do Sul e suas relações com os eventos El Niño e La Niña. Foram utilizados dados de 13 estações de superfície bem distribuídas pelo Estado, no período de 39 anos, compreendido entre 1967 e 2005. As relações entre ondas e ENOS foram obtidas a partir da análise de tabelas de contingência 2 x 2 para os meses centrais de cada estação do ano. A significância estatística foi avaliada através do teste exato de Fischer. Observou-se que a variabilidade sazonal das ondas de frio é diferente da variabilidade das ondas de calor. Ocorrem mais ondas de frio nos meses com as temperaturas mínimas mais baixas, e as ondas de calor têm comportamento mais heterogêneo. Na maioria das localidades, é em julho que as relações entre a ocorrência de ondas de calor e os eventos El Niño são mais significativas. Para La Niña e ondas de Frio, o mês com relações mais significativas foi abril. Em ambos os casos, nota-se que há defasagem entre a ocorrência dos eventos El Niño/La Niña e o seu impacto no surgimento de ondas de calor/frio.This work investigates the seasonal variability occurrence of cold and warm waves in the Brazilian State of Rio Grande do Sul and its relationships with El Niño and La Niña events. 39 years (1967-2005 temperature data series from 13 uniformly distributed meteorological stations over the State were used. The relationships between waves and ENOS were obtained from 2x2 contingence table analysis for the central months of each season. The statistical significance was evaluated using the Fisher exact test. It was observed that the seasonal variability of cold waves is different than the warm wave variability. There are more cold waves during the months with lowest minimum temperatures, and the warm waves have a more heterogeneous behavior. In most localities, the relationships between the occurrence of the warm

  17. Composición y abundancia de artrópodos epígeos del Parque Nacional Llanos de Challe: impactos del ENOS de 1997 y efectos del hábitat pedológico Abundance and composition of epigean arthropods from Llanos de Challe National Park: impacts of ENSO-1997 and effects of the pedological habitat

    Directory of Open Access Journals (Sweden)

    JORGE CEPEDA-PIZARRO

    2005-12-01

    Full Text Available Mediante el uso de trampas de intercepción, se estudió el efecto de la precipitación y el tipo de hábitat sobre la denso-actividad del ensamble de artrópodos epígeos _en particular Tenebrionidae_ del Parque Nacional Llanos de Challe. Este parque está ubicado en el desierto costero transicional de Chile, en la III Región de Atacama. Los muestreos se realizaron durante tres días por mes (septiembre, octubre y diciembre en el período de máxima actividad biológica del sistema y en los años 1989 (año no-ENOS seco, 1997 (año ENOS intenso y 2000 (año no-ENOS húmedo. En las comparaciones se utilizaron dos sitios pedológicamente contrastantes: un hábitat dunario costero y un hábitat pedregoso interior. Se registró la presencia de 15 órdenes de Arthropoda. Los hexápodos constituyeron > 95 % del total de los especímenes capturados (9.065 individuos, siendo Collembola (36,1 % y Coleoptera (29,8 % los órdenes con mayor representatividad numérica. El número de órdenes con representantes activos varió ligeramente entre años: 13 órdenes se registraron en 1989 (44 % de precipitación bajo el promedio, 15 en 1997 (443 % sobre el promedio y 11 en 2000 (52 % sobre el promedio. El efecto del evento ENOS se reflejó claramente en la contribución numérica en la mayoría de los taxa registrados. Aunque más sutilmente, este efecto también se reflejó en la composición del ensamble de taxa dominantes y entre hábitat pedológicos, particularmente con Tenebrionidae y Formicidae. Especialmente en las dunas costeras, Tenebrionidae dominó claramente el ensamble de artrópodos epígeos, siendo Gyriosomus Guérin-Méneville el género más diverso y abundante. La dominancia de Gyriosomus plantea un conjunto de interrogantes respecto de su nivel de endemismo, diversidad y distribución de sus especies, y el papel funcional de estas en el ecosistema estudiadoBy using pitfall traps, the effects of rainfall and habitat type on the denso

  18. Efeitos da atorvastatina e do polimorfismo T-786C do gene eNOS sobre parâmetros do metabolismo lipídico plasmático

    Directory of Open Access Journals (Sweden)

    Vanessa Helena de Souza Zago

    2013-01-01

    Full Text Available FUNDAMENTO: A atividade do óxido nítrico sintase endotelial (eNOS pode ser modulada pelo colesterol da lipoproteína de alta densidade (HDL-C, estatinas ou polimorfismos, como o T-786C de eNOS. OBJETIVO: Este estudo teve como objetivo avaliar se o polimorfismo T-786C está associado a alterações nos efeitos da atorvastatina no perfil lipídico, nas concentrações de metabólitos de óxido nítrico (NO e da proteína C reativa de alta sensibilidade (PCR-as. MÉTODOS: Trinta voluntários do sexo masculino, assintomáticos, com idade entre 18-56 anos foram genotipados e classificados de acordo com a ausência (TT, n = 15 ou presença (CC, n = 15 do polimorfismo. Eles foram selecionados aleatoriamente para a utilização de placebo e atorvastatina (10 mg/dia por 14 dias. Após cada tratamento foram medidos lípides, lipoproteínas, frações HDL2 e HDL3, atividade da proteína de transferência de colesteril éster (CETP, metabólitos de NO e PCR-as. RESULTADOS: As comparações entre genótipos após a administração de placebo mostraram aumento da atividade da CETP polimorfismo-dependente (TT, 12 ± 7; CC, 22 ± 12, p < 0,05. As análises da interação entre os tratamentos indicaram que a atorvastatina tem efeito sobre colesterol, LDL, nitrito e razões lípides/proteínas (HDL2 e HDL3 (p < 0,001 em ambos os genótipos. É interessante notar as interações genótipo/droga sobre a CETP (p < 0,07 e a lipoproteína (a [Lp(a] (p < 0,056, levando a uma diminuição limítrofe da CETP, embora sem afetar a Lp(a. A PRC-as não mostrou alterações. CONCLUSÃO: Os resultados sugerem que o tratamento com estatinas pode ser relevante para a prevenção primária da aterosclerose em pacientes com o polimorfismo T-786C do eNOS, considerando os efeitos no metabolismo lipídico.

  19. Reduction of adhesion molecule production and alteration of eNOS and endothelin-1 mRNA expression in endothelium by Euphorbia hirta L. through its beneficial β-amyrin molecule.

    Science.gov (United States)

    Shih, Mei Fen; Cherng, Jong Yuh

    2014-07-18

    The inflammatory reaction in large blood vessels involves up-regulation of vascular adhesion molecules such as endothelial cell selectin (E-selectin), soluble vascular cell adhesion molecule (sVCAM)-1, and soluble intercellular adhesion molecule (sICAM)-1. These vascular dysfunctions are associated with the development of atherosclerosis. β-Amyrin, an active component of Euphorbia hirta L., has potent anti-inflammatory effects. So far, its preventive effects against the expression of inflammatory mediator-induced adhesion molecules have not been investigated. Endothelial cells (SVEC4-10 cell line) were treated with 50% RAW conditioned media (i.e., normal SVEC4-10 culture media contains 50% of lipopolysaccharide-activated macrophage culture media) without or with β-amyrin (0.6 and 0.3 µM). The production levels of E-selectin, sICAM-1, and sVCAM-1 in the SVEC4-10 cells were measured with ELISA assay kits. Under the same treatment conditions, expression of endothelin (ET)-1 and endothelial type of NO synthase (eNOS) mRNA were analyzed by RT-PCR and agarose gel. With β-amyrin, the 50% RAW conditioned media-induced E-selectin, sICAM-1, and sVCAM-1 levels as well as ET-1 gene expression were all suppressed. β-Amyrin treatment also restored the 50% RAW conditioned media-suppressed eNOS mRNA expression. These data indicate that β-amyrin is potentially useful in preventing chronic inflammation-related vascular diseases.

  20. Effect of normalization of fasting glucose by intensified insulin therapy and influence of eNOS polymorphisms on the incidence of restenosis after peripheral angioplasty in patients with type 2 diabetes: a randomized, open-label clinical trial.

    Science.gov (United States)

    Piatti, Pier Marco; Marone, Enrico; Mantero, Manuela; Setola, Emanuela; Galluccio, Elena; Lucotti, Pietro; Shehaj, Ermal; Villa, Valentina; Perticone, Francesca; Venturini, Massimo; Palini, Alessio; Airoldi, Flavio; Faglia, Ezio; Del Maschio, Alessandro; Colombo, Antonio; Chiesa, Roberto; Bosi, Emanuele; Monti, Lucilla D

    2013-06-01

    Primary objective was to evaluate whether an intensified insulin therapy (IIT) incorporating the target of normal fasting glucose and HbA1c levels could halve the incidence of restenosis/amputation/SCA/death at 6 months after peripheral angioplasty compared with standard care (SC) in patients with type 2 diabetes (DMT2) affected by critical limb ischemia (CLI). Forty-six consecutive patients with DMT2 and CLI were randomly assigned to a parallel, open-label study with IIT (basal-bolus glulisine + glargine administrations) or SC (glargine administration + oral antidiabetic drugs). A SNP of eNOS (rs753482-A>C) and circulating CD34(+) and CD34(+)KDR(+) progenitor cells were determined. At the end of the study, although HbA1c levels were lower in IIT than in SC (6.9 ± 1.3 % vs. 7.6 ± 1.2 %, p incidence of restenosis/amputation/SCA/death (52 and 65 %, respectively, odd ratio 0.59; CI 95 %: 0.21-1.62, p = 0.59). rs753482AC+CC as compared with rs753482AA increased the cumulative incidence of restenosis/amputation/SCA/death (79 and 42 %; odd ratio 5.3; CI 95 %: 1.41-19.5, p incidence of restenosis/amputation/SCA/death in DMT2 and CLI patients. These patients correspond to a class of fragile subjects at high risk of cardiovascular events, and new predictors of restenosis should be contemplated, such as of eNOS polymorphism, (rs753482-A>C SNP) and circulating endothelial progenitor cells.

  1. ibadan, oyo state, nigeria: are patients participating?

    African Journals Online (AJOL)

    Patients who asked their doctors about plan of management significantly reported they had their expectations met. The level of education of the ... issue of the shift was the involvement of the patient in the process of care, including the ..... patient interaction is inadequate despite the high level of satis- faction expressed. '.

  2. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1995. 2. Main issue (Research and development of a human sense index applying technology); 1995 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu seika hokokusho. 2. Honronhen (ningen kankaku shihyo oyo bijutsu no kenkyu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    This paper describes the achievements in fiscal 1995 on research and development of a human sense index applying technology. Total system design and fabrication were launched on a human comfort meter. A resin-made human shaped body attached with warm heat sensing elements and a software logic for a control system for whole body movements were devised. Fabrication was carried out on a driver's seat, a simulated visual field generating device and a system to calculate the machine's movements on a real time basis to perform experiments to evaluate operation reliability by using a 25-ton lifting crane. A system was created that can measure in situ the physiology, psychology and activity reactions. An experimental environment was set that can be operation reliability evaluation indexes by measuring physiological and psychological reactions during crane works, and selection was launched on physiological index candidates. Elucidation was made on characteristics of parallax of both eyes being the main visual function to structure the VR space. At the same time, sensual and physiological verification was given on how important the conditions presented by merged images created by the parallax of both eyes can be in raising the reality of the VR space. The paper also describes the relationship between muscle fatigue and physiological indexes. (NEDO)

  3. A multilevel prediction of physiological response to challenge: Interactions among child maltreatment, neighborhood crime, endothelial nitric oxide synthase gene (eNOS), and GABA(A) receptor subunit alpha-6 gene (GABRA6).

    Science.gov (United States)

    Lynch, Michael; Manly, Jody Todd; Cicchetti, Dante

    2015-11-01

    Physiological response to stress has been linked to a variety of healthy and pathological conditions. The current study conducted a multilevel examination of interactions among environmental toxins (i.e., neighborhood crime and child maltreatment) and specific genetic polymorphisms of the endothelial nitric oxide synthase gene (eNOS) and GABA(A) receptor subunit alpha-6 gene (GABRA6). One hundred eighty-six children were recruited at age 4. The presence or absence of child maltreatment as well as the amount of crime that occurred in their neighborhood during the previous year were determined at that time. At age 9, the children were brought to the lab, where their physiological response to a cognitive challenge (i.e., change in the amplitude of the respiratory sinus arrhythmia) was assessed and DNA samples were collected for subsequent genotyping. The results confirmed that complex Gene × Gene, Environment × Environment, and Gene × Environment interactions were associated with different patterns of respiratory sinus arrhythmia reactivity. The implications for future research and evidence-based intervention are discussed.

  4. Synthesis of lipidated eNOS peptides by combining enzymatic, noble metal- and acid-mediated protecting group techniques with solid phase peptide synthesis and fragment condensation in solution.

    Science.gov (United States)

    Machauer, R; Waldmann, H

    2001-07-02

    Lipid-modified proteins play decisive roles in important biological processes such as signal transduction, organization of the cytoskeleton, and vesicular transport. Lipidated peptides embodying the characteristic partial structures of their parent lipidated proteins and semisynthetic proteins synthesized from such peptides are valuable tools for the study of these biological phenomena. We have developed an efficient synthesis strategy that allows for the synthesis of long multiply lipidated peptides embodying various side chain functional groups. The strategy was successfully applied in the synthesis of the N-terminal undetrigintapeptide of endothelial NO-synthase and related lipopeptides. Key elements of the synthesis strategy are the combined use of the enzyme-labile para-phenylacetoxybenzyloxycarbonyl (PhAcOZ) urethane as N-terminal blocking group, the Pd0-sensitive allyl ester as C-terminal protecting function and acid-labile side chain protecting groups for solution-phase synthesis of labile S-palmitoylated building blocks under the mildest conditions with solid-phase techniques and solution-phase fragment condensations. The successful synthesis of the triply lipidated 29-mer eNOS peptide convincingly demonstrates the full capacity of the protecting group methods.

  5. International Journal of Tropical Agriculture and Food Systems - Vol ...

    African Journals Online (AJOL)

    Wokoma, 197-200 ... Effect of information and communication technology on culture of the people of Saki west local government area of Oyo State, Nigeria · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  6. Growth hormone receptor deficiency in mice results in reduced systolic blood pressure and plasma renin, increased aortic eNOS expression, and altered cardiovascular structure and function

    DEFF Research Database (Denmark)

    Egecioglu, E; Andersson, I J; Bollano, E

    2007-01-01

    To study the role of the growth hormone receptor (GHR) in the development of cardiovascular structure and function, female GHR gene-disrupted or knockout (KO) and wild-type (WT) mice at age 18 wk were used. GHR KO mice had lower plasma renin levels (12 +/- 2 vs. 20 +/- 4 mGU/ml, P .... These data suggest an important role for an intact GH/IGF-I axis in the maintenance of a normal cardiovascular system....

  7. Assessing Library Automation and Virtual Library Development in Four Academic Libraries in Oyo, Oyo State, Nigeria

    Science.gov (United States)

    Gbadamosi, Belau Olatunde

    2011-01-01

    The paper examines the level of library automation and virtual library development in four academic libraries. A validated questionnaire was used to capture the responses from academic librarians of the libraries under study. The paper discovers that none of the four academic libraries is fully automated. The libraries make use of librarians with…

  8. Application of A1203-Cr203 clinker to sliding nozzle plate. Cr203 koyo A1203 kurinka no sliding nozuru plate eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Nitawaki, S.; Shikano, H.; Tanaka, I. (Kurosaki Refractories Co. Ltd., Fukuoka (Japan))

    1992-10-10

    With the objective to improve the corrosion resistance of the SN plate of Al2O3-metal Al-C system, Al2O3 clinker with solute is Cr2O3 is prepared and applied to bricks. As a result, the reduction of Cr2O3 is suppressed with minimum structural degradation by the application of coarse grain Al2O3 clinker with Cr2O3 solute in the ratio of 2%, the corrosion resistance being improved by 12 to 17%. The substances with 2% and 5% solute Cr2O3 are used as the starting substances for the prepared AC clinker, which are hereunder called AC2 and AC5, respectively. In general, AC0 which has no solute Cr2O3 seems to have higher solubility to slag in comparison to AC2 and AC5 with solid solute Cr2O3. The reason seems to be the reaction of small amount of Cr2O3 with slag to form highly viscous protective film which prevents bricks and slag from being moistened. 4 refs., 6 figs., 4 tabs.

  9. The Redox-sensitive Induction of the Local Angiotensin System Promotes Both Premature and Replicative Endothelial Senescence: Preventive Effect of a Standardized Crataegus Extract.

    Science.gov (United States)

    Khemais-Benkhiat, Sonia; Idris-Khodja, Noureddine; Ribeiro, Thais Porto; Silva, Grazielle Caroline; Abbas, Malak; Kheloufi, Marouane; Lee, Jung-Ok; Toti, Florence; Auger, Cyril; Schini-Kerth, Valérie B

    2016-12-01

    Endothelial senescence, characterized by an irreversible cell cycle arrest, oxidative stress, and downregulation of endothelial nitric oxide synthase (eNOS), has been shown to promote endothelial dysfunction leading to the development of age-related vascular disorders. This study has assessed the possibility that the local angiotensin system promotes endothelial senescence in coronary artery endothelial cells and also the protective effect of the Crataegus extract WS1442, a quantified hawthorn extract. Serial passaging from P1 to P4 (replicative senescence) and treatment of P1 endothelial cells with the eNOS inhibitor L-NAME (premature senescence) promoted acquisition of markers of senescence, enhanced ROS formation, decreased eNOS expression, and upregulation of angiotensin-converting enzyme (ACE) and AT1 receptors. Increased SA-β-gal activity and the upregulation of ACE and AT1R in senescent cells were prevented by antioxidants, an ACE inhibitor, and by an AT1 receptor blocker. WS1442 prevented SA-β-gal activity, the downregulation of eNOS, and oxidative stress in P3 cells. These findings indicate that the impairment of eNOS-derived nitric oxide formation favors a pro-oxidant response triggering the local angiotensin system, which, in turn, promotes endothelial senescence. Such a sequence of events can be effectively inhibited by a standardized polyphenol-rich extract mainly by targeting the oxidative stress. © The Author 2015. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. Factors responsible for subclinical mastitis in cows caused by Staphylococcus chromogenes and its susceptibility to antibiotics based on bap, fnbA, eno, mecA, tetK, and ermA genes.

    Science.gov (United States)

    Bochniarz, M; Adaszek, Ł; Dzięgiel, B; Nowaczek, A; Wawron, W; Dąbrowski, R; Szczubiał, M; Winiarczyk, S

    2016-12-01

    The aim of this study was to recognize selected factors of virulence determining the adhesion of Staphylococcus chromogenes to cows' udder tissues in subclinical mastitis and to evaluate the susceptibility of this pathogen to antibiotics. The subjects of the study were 38 isolates of Staph. chromogenes from 335 samples of milk from cows with subclinical coagulase-negative staphylococci mastitis. Somatic cell count ranged between 216,000 and 568,000/mL of milk (average 356,000/mL of milk). We confirmed the ability to produce slime in 24 isolates (63.2%), and the ability to produce protease in 29 isolates (76.3%). In each slime-producing isolate, the bap gene was not found, and the fnbA and eno genes were not detected. In vitro tests showed that ceftiofur had the highest effectiveness against Staph. chromogenes (89.5% of susceptible isolates). Minimum inhibitory concentrations ranged from 0.06 to 2µg/mL for susceptible isolates. The minimum concentrations required to inhibit growth of 90 and 50% of the isolates for ceftiofur were at or below the cutoffs recommended by the Clinical and Laboratory Standards Institute (2 and 0.06µg/mL, respectively). A significant percentage of the isolates were susceptible to other β-lactam antibiotics: amoxicillin with clavulanic acid (84.2%) and ampicillin (81.6%). The lowest effectiveness among β-lactams was for penicillin (73.7% of susceptible isolates), and the minimum inhibitory concentration for penicillin ranged from <0.06 to 8µg/mL. None of the examined isolates had the mecA gene, but β-lactamase was detected in 4 isolates (10.5%). Erythromycin and oxytetracycline exhibited the lowest activity against Staph. chromogenes (71.1 and 63.2% of susceptible isolates, respectively). The genes tetK (6 isolates) and ermA (1 isolate) were also detected. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  11. Mechanistic link between erectile dysfunction and systemic endothelial dysfunction in type 2 diabetic rats.

    Science.gov (United States)

    Musicki, B; Hannan, J L; Lagoda, G; Bivalacqua, T J; Burnett, A L

    2016-09-01

    Men with type 2 diabetes mellitus (T2DM) and erectile dysfunction (ED) have greater risk of cardiovascular events than T2DM men without ED, suggesting ED as a predictor of cardiovascular events in diabetic men. However, molecular mechanisms underlying endothelial dysfunction in the diabetic penis explaining these clinical observations are not known. We evaluated whether the temporal relationship between ED and endothelial dysfunction in the systemic vasculature in T2DM involves earlier redox imbalance and endothelial nitric oxidase synthase (eNOS) dysfunction in the penis than in the systemic vasculature, such as the carotid artery. Rats were rendered T2DM by high-fat diet for 2 weeks, followed by an injection with low-dose streptozotocin. After 3 weeks, erectile function (intracavernosal pressure) was measured and penes and carotid arteries were collected for molecular analyses of eNOS uncoupling, protein S-glutathionylation, oxidative stress (4-hydroxy-2-nonenal, 4-HNE), protein expression of NADPH oxidase subunit gp91(phox) , endothelium-dependent vasodilation in the carotid artery, and non-adrenergic, non-cholinergic (NANC)-mediated cavernosal relaxation. Erectile response to electrical stimulation of the cavernous nerve and NANC-mediated cavernosal relaxation was decreased (p penis, but not in the carotid artery, of T2DM compared to non-diabetic rats. In conclusion, redox imbalance, increased oxidative stress by NADPH oxidase, and eNOS uncoupling, occur early in T2DM in the penis, but not in the carotid artery. These molecular changes contribute to T2DM ED, while vascular function in the systemic vasculature remains preserved. © 2016 American Society of Andrology and European Academy of Andrology.

  12. Progress of electrical and electronic engineering in the last 10 years. 6. Progress in the field of industry applications; Denki denshi gijutsu 10nen no ayumi-6. Sangyo oyo bun`ya ni okeru ayumi

    Energy Technology Data Exchange (ETDEWEB)

    Nakazato, T.

    1998-06-01

    Power electronics technology conducting conversion and control of electric power by means of electronic means by using the power semiconductor device, developed extremely by development of the power transistor and GTO (Gate Turn-off Thyristor). In the demand, the spread of inverter is miracle, centering the motor control, uninterruptible power supply (USP) and so on, it is used in many fields such as manufacturing industry, chemical industry, OA (Office Automation) system, and so forth. The history of realization in the rotary machines reaches about 110 years. The development of recent technique containing driving technique is miracle. There is a variable speed pumped-storage power system developed and realized by Japan faster than in the world. In the field of the railway, speed-up in the Shinkansen line and the ordinary railway line, power saving, cost reduction, improved convenience, and so forth were intended. In the field of the road traffic, car navigation brought an extreme spread as well as cost reduction. For a typical example of control technique in industry, there is a technique of motion control. This is a field possible for Japan to show a priority from its original, such as robot control. 6 figs., 1 tab.

  13. Introduction of anti-rolling active vertical fin and its application to maneuverability for displacement-type super high speed ship. 2nd Report.; Haisuiryogata chokosokusen no yokoyure seishiyo active suichoku fin no donyu to sojuseieno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hirayama, T.; Saito, Y. [Yokohama National University, Yokohama (Japan). Faculty of Engineering; Niihara, Y. [Ishikawajima-Harima Heavy Industries Co. Ltd., Tokyo (Japan)

    1996-12-31

    Discussed herein are the effects of vertical fin projecting downwards from the bottom of a displacement-type superhigh-speed ship, installed to improve its transverse stability and turning ability. The system for simultaneously controlling rudder and vertical fin by the optimum regulator was studied for maneuverability in directional following waves and smooth water, and maneuverability was simulated numerically. A ship is greatly rolled and sloped when running in waves. It is found that the vertical fin shows a high anti-rolling effect when the ship runs straight. The optimum regulator greatly improves maneuverability in waves, reducing rolling by 92%. Increased rolling with the vertical fin, observed in the previous study in directional following waves, is found to be due to the rudder. The optimum position of the fin is determined to control transverse sloping of a turning ship in a superhigh-speed region. 21 refs., 12 figs., 4 tabs.

  14. Synthesis of {epsilon}-caprolactam having stable nitroxyl radical at terminal of chain and its application to living radical polymerization; Satanmatsu ni antei na nitorokishirajokaru wo yusuru ({epsilon}-kapurorakuton) no gosei to ribingurajikaru jugo heno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Mayumi. [Tokyo Institute of Technology, Tokyo (Japan)

    1999-06-01

    Radical polymerization is one of the most widely used industrial polymerizations since it can be applied to numerous kinds of monomers and the reaction system thereof is easily handled. Owever, it has a defect that the radicals at the growing terminal tend to have a side reaction. In recent years, it has been found that, in radical polymerization of styrene, when stable nitroxyl radicals are made to exist in the polymerization and the temperature is raised, the nitroxyl groups bonded to the growing chain terminal are disassociated to regenerate radicals, thus living polymerization proceeds. Yoshida et al. have attempted the living polymerization of styrene using TEMPO introduced to the terminal of a polymer chain as counter radical instead of low molecule TEMPO used hitherto. In the living radical polymerization using TEMPO, a new type block copolymer with poly CL bonded to the stop terminal of styrene via TEMPO is synthesized. (NEDO)

  15. Synthesis of [epsilon]-caprolactam having stable nitroxyl radical at terminal of chain and its application to living radical polymerization. Satanmatsu ni antei na nitorokishirajokaru wo yusuru ([epsilon]-kapurorakuton) no gosei to ribingurajikaru jugo heno oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Mayumi. (Tokyo Institute of Technology, Tokyo (Japan))

    1999-06-01

    Radical polymerization is one of the most widely used industrial polymerizations since it can be applied to numerous kinds of monomers and the reaction system thereof is easily handled. Owever, it has a defect that the radicals at the growing terminal tend to have a side reaction. In recent years, it has been found that, in radical polymerization of styrene, when stable nitroxyl radicals are made to exist in the polymerization and the temperature is raised, the nitroxyl groups bonded to the growing chain terminal are disassociated to regenerate radicals, thus living polymerization proceeds. Yoshida et al. have attempted the living polymerization of styrene using TEMPO introduced to the terminal of a polymer chain as counter radical instead of low molecule TEMPO used hitherto. In the living radical polymerization using TEMPO, a new type block copolymer with poly CL bonded to the stop terminal of styrene via TEMPO is synthesized. (NEDO)

  16. Fiscal 1998 R and D report on human feeling measurement application technology. Pt. 1. Outline; 1998 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu itaku kenkyu seika hokokusho. 1. Gaiyohen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    This report outlines the fiscal 1998 R and D result on human feeling measurement application technology. For development of assessment technology of the impact of work fatigue on human feeling (human feeling index), and assessment technology of the adaptability and affinity between human being and environment or product (environment and product adaptability index), data storage and evaluation by measuring experiment of human feeling, and modification of every index toward the final index based on the above data were carried out. Further case studies were carried out to reflect the above both indices to design of living products or residence and office environments, and new data were also collected. The database model for using previously collected human feeling data effectively, and the sweating manikin for estimating human thermal feeling reasonably were developed. In addition, the human feeling measurement manual was prepared to diffuse these technologies. The R and D system is also described. (NEDO)

  17. Achievement report for fiscal 1997 on the research and development of human sensory measurement application technology. 2. Subject; 1997 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu seika hokokusho. 2. Honronhen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    Efforts continue from the previous fiscal year to analyze time-dependent correlations between changes in fatigue/alertness-related psychological quantities and combined physiological changes. Using an office space as a sample, an augmented model is completed concerning a relative method of measurement, analysis of impression structure, scaling of the degree, and simultaneous relationship analysis. The environmental compatibility indexing is improved by analyzing physical quantities and brain waves that influence psychological sensation. In the indexing of product compatibility wherein the compatibility between products and human beings is evaluated from the viewpoint of affinity or friendliness, the index structure dependent on subjectivity and physiological reaction is enhanced in precision. Application case studies in which indices of physiological sensation, environments, and product compatibility are reflected on the designs of daily-use products and living/working circumstances, and a human sensory database prototype is experimentally constructed. The indexing prototype, related to muscular fatigue due to a restrained working attitude and mental fatigue due to the load of mental activities, is enhanced in precision. In the study of product compatibility indexing technology, improvement and evaluation are carried out on the practical attitude/sight line evaluation system. (NEDO)

  18. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1993. 1. Outline; 1993 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu. 1. Soron

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    This paper outlines the development of a technology to apply human senses to measurements in fiscal 1993. Using the period from fiscal 1990 through 1994 as the first term, development is made for physiological effect measurement on a technology to measure in a simplified manner the physiological changes as brainwave and pupil reactions and induction potential caused by external stimulation. For the simulated environment presenting technology, devices and systems are structured that can generate and control collectively such externally stimulated environments as heat, sound, vibration, light beam, scent, scenery, and constituting materials. The technology to investigate correlation among the externally stimulated environments, physiological effects, and sense volume establishes a testing technology to acquire efficiently the statistical correlation data. Achievements obtained by fiscal 1993 may be summarized as follows: a non-contact non-invasion measuring device was fabricated on a trial basis to identify quantitatively the sense volume attributed from external stimulation, and the device was given experiment, evaluation and improvement; with regard to the simulated environment presentation, partially detailed design, fabrication and experiment were performed on a device that can effectively present the subjects with such environments as sound and space; and in the correlation investigation technology, the basic experiments have resulted in a simulator to evaluate correlation among stress, fatigue and arousal, and correlation between spatial environment and visual information. (NEDO)

  19. Achievement report for fiscal 1998 on the research and development of human sensory measurement application technology. 2. Subject 1; 1998 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu seika hokokusho. 2. Honronhen 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Indexing systems are proposed which cover the combined physiological effects of fatigue and alertness as experienced under typical working conditions. Efforts are made at standardizing measurement and at developing the results into a database. Concerning the impression generated by things and space, programs for standardizing impression measuring techniques on the free declaration basis and for supporting such techniques are completed. A structural impression model construction method is embodied. Based on the visual psychological/physiological quantities and the physical quantity of texture, an indexing equation is completed for indexing the visual sensation created by textile products. Application cases are expanded in which the indices involving physiological sensation, environments, and product compatibility are reflected on the designs of daily-use commodities and living/working circumstances, and new data are accumulated. A perspiration-capable mannequin is completed which enables the estimation of man's sensation upon exposure to heat. For the indexing of physiological sensation, studies are conducted for the improvement of fatigue indexing technological infrastructure and for the indexing of alertness. Mentioned also are environmental compatibility indexing, product compatibility indexing, human sensation database models and measuring manuals, and experiments of application of restful environments. (NEDO)

  20. Achievement report for fiscal 1996 on the research and development of human sensory measurement application technology. 2. Subject; 1996 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu seika hokokusho. 2. Honronhen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    In relation with fatigue indexing basics in the study of physiological sensation indexing technologies, an indexing system prototype is built, covering muscular fatigue due to a restrained working attitude and mental fatigue due to the load of psychological activities. Features of each fatigue/alertness stage are analyzed, and the time-dependent correlations between the change in psychological quantities and the combined physiological change is also analyzed. In the study of environmental compatibility indexing, color temperatures of office interior, amenity, and spatial dimension indexing are taken up. The impression of office space is analyzed. Highly reliable technologies in pursuit of psychological sensation are established, and efforts are made at indexing environmental compatibility by analyses of physical quantities affecting psychological sensation, and of brain waves. Test methods for habituation to noise are discussed, and relations between the sensations of habituation and fatigue/obstruction are quantified. In relation with product compatibility indexing technologies, products' compatibility with operator's attitude, the line of sight, and loads on human beings is evaluated. Discussed also is the application of the indices involving physiological sensation, environments, and product compatibility to products for daily life and to the designing of living and working environments. (NEDO)

  1. The effect of acute exposure to hyperbaric oxygen on respiratory system mechanics in the rat.

    Science.gov (United States)

    Rubini, Alessandro; Porzionato, Andrea; Zara, Susi; Cataldi, Amelia; Garetto, Giacomo; Bosco, Gerardo

    2013-10-01

    This study was designed to investigate the possible effects of acute hyperbaric hyperoxia on respiratory mechanics of anaesthetised, positive-pressure ventilated rats. We measured respiratory mechanics by the end-inflation occlusion method in nine rats previously acutely exposed to hyperbaric hyperoxia in a standard fashion. The method allows the measurements of respiratory system elastance and of both the "ohmic" and of the viscoelastic components of airway resistance, which respectively depend on the newtonian pressure dissipation due to the ohmic airway resistance to air flow, and on the viscoelastic pressure dissipation caused by respiratory system tissues stress-relaxation. The activities of inducible and endothelial NO-synthase in the lung's tissues (iNOS and eNOS respectively) also were investigated. Data were compared with those obtained in control animals. We found that the exposure to hyperbaric hyperoxia increased respiratory system elastance and both the "ohmic" and viscoelastic components of inspiratory resistances. These changes were accompanied by increased iNOS but not eNOS activities. Hyperbaric hyperoxia was shown to acutely induce detrimental effects on respiratory mechanics. A possible causative role was suggested for increased nitrogen reactive species production because of increased iNOS activity.

  2. Modeling Selected Climatic Variables in Ibadan, Oyo State, Nigeria ...

    African Journals Online (AJOL)

    PROF. O. E. OSUAGWU

    2013-09-01

    Sep 1, 2013 ... skewed real life data sets. Since the total rainfall and temperature data of Ibadan under consideration are skewed, the choice of this density function is considered appropriate for the study at hand. 2.0 The Modified Generalized Burr Density Function. [18] defined the generalized burr density function as.

  3. Determinants of adoption of soil conservation practices in Oyo State ...

    African Journals Online (AJOL)

    The truncated negative binomial Count Data model was used to identify factors influencing adoption of the practices. Factors identified as determinants of their adoption of soil conservation practices include: farm size, land tenure, extension contact, household net worth, awareness, perceived benefits. These variables are ...

  4. Assessment of support zone community programme in Old-Oyo ...

    African Journals Online (AJOL)

    ATBU Journal of Environmental Technology. Journal Home · ABOUT · Advanced Search · Current Issue · Archives · Journal Home > Vol 8, No 1 (2015) >. Log in or Register to get access to full text downloads.

  5. Assessment of frog meat utilisation in Ibadan, Oyo state, Nigeria ...

    African Journals Online (AJOL)

    All the respondents (100%) buy the frogs utilised, have knowledge of the sellers and were unaware of frog farms in Nigeria. All the respondents (100%) considered frog meat desirable and preferred it when dried. Almost all the respondents (98.0%) have consumed frog meat more than 10 times. Majority of the respondents ...

  6. Spatial Modelling of Road Traffic Accidents in Oyo State, Nigeria ...

    African Journals Online (AJOL)

    ... on average approximately one accident for the period under study. Travel densities are negatively related to number of accidents, which suggests inhibiting factors in the sense that traffic generated tend to be associated with fewer crashes. Keywords: Road traffic accidents, spill over effect, spatial lag modelling, maximum ...

  7. Analysis of the Physicochemical Burden of Oyo State Fish Pond ...

    African Journals Online (AJOL)

    CCWW was brownish in colour and slightly smelly and fishes were sighted. OYSFP ... BDJWW was clear, no odour, animal life forms including fish and surface ... in OYSFP and fish produced there may not be suitable for human consumption.

  8. Information seeking behaviour of fish farmers in Oyo State | Akinbile ...

    African Journals Online (AJOL)

    The technical nature of fish farming requires that the knowledge of practitioners are constantly updated and this can be achieved through their enhanced information seeking behaviour as information use of fish farmers is crucial for effectively meeting their information needs. This study, thus, determined the information ...

  9. Flood occurrence and adjustment strategies in Oyo State: a case ...

    African Journals Online (AJOL)

    Floods are among the most dramatic forms of interaction between man and his environment, and they emphasize the limitations of man in his attempt to control the sheer force of nature. When they occur, whether in the developed or the developing world, they are always associated with heavy losses of life and property, ...

  10. Determinants of job effectiveness of extension personnel in Oyo ...

    African Journals Online (AJOL)

    % were PhD holders. Majority (55%) had worked between 5-10 years with 62.5% earned between #41,000 and #80,000. Personnel were mostly effective in professional work performance ((X=4.35) and competency ((=4.28). Incentive to job ...

  11. Helminthic infections among farmers in a rural community in Oyo ...

    African Journals Online (AJOL)

    Background: Helminthic infections are occupationally-related diseases which potentially undermine farmers' work capacity, productivity and life expectancy. These infections are usually under-reported among this group particularly in the rural areas. This study was carried out to determine the prevalence, pattern and factors ...

  12. Phenotypic Characterization of the Bunaji Cattle Breed in Oyo State ...

    African Journals Online (AJOL)

    The phenotypic traits observed indicated that Bunaji breed of cattle were predominantly white (78%) in color, while grey and pied are less common (2 and 1% respectively). Ear type was medium and erect with dewlap and penile sheath being prominent. Keywords: Phenotypic, characterization, Bunaji cattle. Sahel Journal ...

  13. Yellow fever vaccination in Nigeria: focus on Oyo State | Onoja ...

    African Journals Online (AJOL)

    Background: National vaccination campaigns prevented yellow fever virus epidemics in Nigeria. A build-up of Aedes aegypti mosquitoes have been found in parts of the rain forest region that was the hotbed of previous epidemics. This study provides information on the annual vaccination counts in some major vaccination ...

  14. Elemental Concentration of Harmattan Dust Sample in Iwo and Oyo ...

    African Journals Online (AJOL)

    ADOWIE PERE

    nostrils as a result of the dust the blow across the country. This fact has been pointed out by various meteorological observers Samway, (1975). High pressure to the north of Bodele Depressions intensifies the north easterly trade winds leading to an increase entrainment of dust in the Bodele. Depression Adedokun et al., ...

  15. Modeling Selected Climatic Variables in Ibadan, Oyo State, Nigeria ...

    African Journals Online (AJOL)

    The choice of the density function was based on the positive skewness of the two climate series, a sufficient condition for the selection of the burr density. The Kolmogorov-Smirnov goodness of fit tests and the plots of the cumulative distribution function confirmed the adequacy of the density function to model adequately the ...

  16. Acceptability of Edible Mushroom as a Dietary Supplement in Oyo ...

    African Journals Online (AJOL)

    This study focused on the acceptability of edible mushroom as a dietary supplement. Virtually all the respondents (96.6 %) know what mushrooms are, but only half (55.2 %) consumes it. Less than half of the respondents (34.5 %) know the dietary components/benefits of mushroom. A greater majority of the respondents ...

  17. Occurrence of Klebsiella Species in Cultured African Catfish in Oyo ...

    African Journals Online (AJOL)

    Fish is capable of habouring diseases and therefore, must be safe and free of infectious pathogens if aquaculture should fulfill its potential. Infectious disease has being one of the major causes of death worldwide in recent times. Klebsiella species in the family of enterobacteriacea causes urinary tract infections, pneumonia ...

  18. Determinants of production level of commercial snail farmers in Oyo ...

    African Journals Online (AJOL)

    Analytical tools employed include frequency counts, Chi-Square and five (5) point Likert scale. The results show that only 23.90% of the respondents are females while about 74% of the respondents had herd size of less than 1000 snails. Snail farmers with tertiary education are more in the distribution (44.10%) while civil ...

  19. Determinants of Child Mortality in Oyo State, Nigeria

    African Journals Online (AJOL)

    Toshiba

    explaining infant (under 12 months) mortality, socioeconomic, socio- cultural and hygienic factors are more important in explaining child. (under five) mortality. Younger (2007), however, do not find significant effects of variables related to the quality of drinking water and of sanitation on infant mortality. Vol. 8 (1) Serial No.

  20. Estimation of Magnetic Basement Depth of Oyo Area from ...

    African Journals Online (AJOL)

    objective of this study is to define the depth to the crystalline basement in the study area by means of the magnetic automated processing techniques. Locations and depths to magnetic contacts were estimated from the total intensity magnetic field using Horizontal gradient magnitude (HGM), Analytic signal amplitude (ASA) ...

  1. El niño, oscilación del sur (ENOS, con relación a la transmisión de malaria, densidad y paridad de An. albimanus y An. darlingi (diptera: culicidae en dos regiones de Colombia

    Directory of Open Access Journals (Sweden)

    Ricardo Mantilla

    2000-02-01

    Full Text Available

    Una fuerte asociación ha sido registrada entre los incrementos en la transmisiónas forzadas por el fenómeno El Niño. Entre las posibles explicaciones para esta asociación está el efecto del clima sobre la dinámica de población de vectores, por ejemplo, generando cambios en la densidad y en las tasas de sobrevivencia de la población, y en la cantidad y calidad de sitios de reproducción, como también a través de cambios en el período de incubación extrínseco del parásito dentro del vector. Con el propósito de evaluar la influencia de ENOS sobre la densidad y paridad de Anopheles albimanus y An. darlingi, y su relación con la transmisión de malaria, se realizó un estudio longitudinal en Chocó y Casanare. En cada área se seleccionaron dos localidades y se realizaron muestreos entomológicos durante una semana cada mes, desde finales de 1997 hasta 1999. Este período cubrió los eventos El Niño 1997 -1998 y La Niña 1998 - 2000. En las localidades de Chocó se observó un importante incremento en el número de casos de malaria durante el evento ENOS, asociado con la temperatura. No se observó una asociación estadísticamente significativa entre los casos de malaria y la precipitación o la humedad relativa. La relación entre el incremento de malaria durante El Niño y las variables entomológicas no fue evidente. La densidad y paridad de ambas especies de vectores fluctuaron durante el período de estudio y no se encontró una asociación significativa entre estas variables y los factores climáticos como la temperatura. La precipitación y la humedad relativa. La densidad de larvas de An. albimanus fluctuó durante el estudio, y mostró altas densidades alrededor de julio - agosto. No se observó asociación entre la densidad de larvas con la densidad de adultos o las variables climáticas. El efecto del evento ENOS sobre las poblaciones de vectores

  2. Aurantio-obtusin relaxes systemic arteries through endothelial PI3K/AKT/eNOS-dependent signaling pathway in rats

    Directory of Open Access Journals (Sweden)

    Shuzhen Li

    2015-07-01

    Full Text Available Aurantio-obtusin is a natural effective compound isolated from Semen Cassiae, which possesses hypotensive and hypolipidemic effects. Although its hypotensive effect have been clarified, mechanisms Aurantio-obtusin relaxes systemic arteries remain unclear. This study was to investigate effects and mechanisms of Aurantio-obtusin on isolated mesenteric arteries (MAs. We examined MAs relaxation induced by Aurantio-obtusin on rat isolated MAs, expression and activity of endothelial nitric oxide synthase (eNOS and protein kinase B (AKT, and nitric oxide (NO production in bovine artery endothelial cells (BAECs. Findings showed Aurantio-obtusin elicited dose-dependent vasorelaxation with phenylephrine (PE precontracted rat MA rings (diameter: 200–300 μm, which can be diminished by denudation of endothelium and inhibition of eNOS activity, while having no effect on rat isolated pulmonary artery (PA rings. Aurantio-obtusin increased NO production by promoting phosphorylations of eNOS at Ser-1177 and Thr-495 in endothelial cells. Aurantio-obtusin also promoted phosphorylations of Akt at Ser-473. PI3K inhibitor LY290042 could diminish vasorelaxation induced by Aurantio-obtusin. Moreover Aurantio-obtusin also elicited dose-dependent vasorelaxation effect with PE precontracted MA rings (diameter: 100–150 μm. Therefore, vasorelaxation induced by Aurantio-obtusin was dependent on endothelium integrity and NO production, which mediated by endothelial PI3K/Akt/eNOS pathway. Results suggest Aurantio-obtusin may offer therapeutic effects in hypertension, as a new potential vasodilator.

  3. Development of programmable multi-channel earth resistivity system

    Energy Technology Data Exchange (ETDEWEB)

    Chung, Hyun Ki; Choi, Jong Ho; Park, In Wha [Korea Institute of Geology Mining and Materials, Taejon (Korea, Republic of)

    1996-12-01

    Maximum 256 channel digital-stacking automatic electrical earth resistivity meter is upgrade-developed and field-tested with two commercially available systems (OYO McOHM and ABEM Terrameter) for Schlumberger vertical sounding and dipole-dipole arrays. The results of three systems are very well coincident for several dummy resistors and Schlumberger array in field site. The developed system K-Ohm shows even more reasonable quality data in sensitive dipole-dipole array measurements in comparison with the electrical survey instruments of digital stacking type manufactured by other countries. New Important features of upgraded programmable K-Ohm system are as follows ; 1) Auto-electrode-switching control by Notebook printer port, 2) receiving signal measurement by Notebook serial port, 3) interactive automatic dipole-dipole measurement software with two apparent resistivity sections compared in one Notebook display to minimize noisy data in field, 4) auto-saved field memo at any time appending to acquired data, 5) max 500 V{sub p-p} 500 mA transmitter (measuring cycle S/W programmable), 6) low-drift sigma - delta 24 bit A/D 0.0015 % linearity error with zero-offset and full - scale gain autocalibration, 7) DC 12 v operated and TX-RX 7,000 V optical-isolated, 8) electrodes grounding auto-tested, user-oriented any array sequential programmable control software. Further study will be focused on higher power TX and stand alone TX-RX system, and micro-resistivity system for in-borehole resistivity imaging. (author). 8 refs., 9 figs.

  4. Geographical information system (GIS) mapping of spatio-temporal pollution status of rivers in Ibadan, Nigeria.

    Science.gov (United States)

    Adeyemo, Olanike K; Babalobi, Olutayo O

    2008-04-01

    More accurate spatio-temporal predictions of urban environment are needed as a basis for assessing exposures as a part of environmental studies and to inform urban protection policy and management. In this study, an information system was developed to manage the physico-chemical pollution information of Ibadan river system, Oyo State, Southwest Nigeria. The study took into account the seasonal influences of point and non-point discharges on the levels of physico-chemical parameters. The overall sensitivity of the watershed to physicochemical environmental pollution revealed that during dry season, of the 22 (100%) sample points, only 3 (13.6%) were unpolluted; 6 (27.3%) were slightly polluted; 10(45.4%) were moderately polluted; 2 (9.1%) were seriously polluted and 1 (4.5%) was exceptionally polluted. During rainy season, 3 (13.6%) were unpolluted; 7 (31.8%) were slightly polluted; 9 (40.9%) were moderately polluted; 2 (9.1%) were seriously polluted and 1 (4.5%) was exceptionally polluted. There is a considerable environmental risk associated with the present level of pollution of the Ibadan river water body on fish health and biodiversity. This research provides a basis for aquatic management and assist in policy making at national and international levels. Appropriate strategies for the control of point and non-point pollution sources, amendments and enforcement of legislation should be developed.

  5. USE AND USE INTENSITY OF SOCIAL MEDIA NETWORKING SYSTEMS BY NIGERIAN AGRO-ENTREPRENEURS

    Directory of Open Access Journals (Sweden)

    Joseph Omotoso AJAYI

    2015-04-01

    Full Text Available Agro-entrepreneurs are key to the Nigerian economy as they constitute the majority of the work force, providing food and employment opportunities for more than 60% of the largest black nation on earth. Nigeria’s social media networking system which is the largest in Africa has got its usefulness in many conventional and contemporary applications in the recent times. Social media has become the fastest, cheapest and unrestricted communication and marketing tool of this jet age. This study therefore focused on the use and use intensity of social media networking systems by Nigerian agro-entrepreneurs. The study made use of mainly primary data. Primary data were collected with the aid of well-structured questionnaires assisted with interview schedules. Field data collection was conducted between June and September, 2014. Multi-stage sampling technique was used to select three hundred and sixty (360 respondents across the three (3 highly ICT compliant states (Lagos, Ondo and Oyo. Data collected were analysed using descriptive statistics and multiple regression model. The results of the regression model indicate that education, availability of power supply and customer base of the agro-entrepreneurs positively influenced use intensity of social media by the agro-entrepreneurs while age and cost of access data negatively influenced their use intensity of social media.

  6. systems

    Directory of Open Access Journals (Sweden)

    Alexander Leonessa

    2000-01-01

    Full Text Available A nonlinear robust control-system design framework predicated on a hierarchical switching controller architecture parameterized over a set of moving nominal system equilibria is developed. Specifically, using equilibria-dependent Lyapunov functions, a hierarchical nonlinear robust control strategy is developed that robustly stabilizes a given nonlinear system over a prescribed range of system uncertainty by robustly stabilizing a collection of nonlinear controlled uncertain subsystems. The robust switching nonlinear controller architecture is designed based on a generalized (lower semicontinuous Lyapunov function obtained by minimizing a potential function over a given switching set induced by the parameterized nominal system equilibria. The proposed framework robustly stabilizes a compact positively invariant set of a given nonlinear uncertain dynamical system with structured parametric uncertainty. Finally, the efficacy of the proposed approach is demonstrated on a jet engine propulsion control problem with uncertain pressure-flow map data.

  7. High-performance liquid chromatography/mass spectrometric identification of dibenzylbutyrolactone-type lignans: insights into electrospray ionization tandem mass spectrometric fragmentation of lign-7-eno-9,9'-lactones and application to the lignans of Linum usitatissimum L. (Common Flax).

    Science.gov (United States)

    Schmidt, Thomas J; Alfermann, A Wilhelm; Fuss, Elisabeth

    2008-11-01

    In continuation of our studies into the mass spectrometric detection of natural lignans and their identification in complex mixtures such as crude plant extracts, the electrospray ionization tandem mass spectrometric (ESI-MS/MS) fragmentation of Delta(7,8)-unsaturated dibenzylbutyrolactone-type lignans (lign-7-eno-9,9'-lactones) was studied in detail. It is demonstrated that the characteristic fragmentation allows unambiguous identification including distinction between constitutional isomers. These lignans containing an alpha,beta-unsaturated lactone structure exist as equilibrium mixtures of E- and Z-isomers indistinguishable by mass spectrometry, but it is shown that chromatographic retention time can be used to distinguish between the isomeric forms. Based on these observations, re-analysis of the dichloromethane extract obtained from flowering aerial parts of Linum usitatissimum L. by high-performance liquid chromatography (HPLC)/ESI-MS/MS led to the identification of eighteen lignans of these types (five lignano- and one lignenolactone previously reported along with five further lignano- as well as seven lignenolactones hitherto unreported for this plant). The simultaneous identification of eighteen different lignans in the complex matrix of a crude plant extract by a single analysis demonstrates the potential of this method, which will certainly lead to new insights into the lignan composition and metabolism of different Linum species and many other plants.

  8. Measures for global environmental issues using information communication. Results of trial calculation of CO2 reduction reported by the Telecommunication Council; Joho tsushin wo katsuyoshita chikyu kankyo mondai eno taio. Denki tsushin shingikai toshin no CO2 sakugen shisan kekka

    Energy Technology Data Exchange (ETDEWEB)

    Fujiwara, T. [Ministry of Posts and Telecommunications, Tokyo (Japan)

    1998-11-01

    Telework is an effective work style using information communication without commutation, and ITS is an integrated traffic system such as advanced navigation. Effects of the CO2 emission reduction by these information communication systems were calculated as a trial. Reduction of CO2 4.06 million ton was obtained, which is equivalent to about 7% of the reduction target 56.5 million ton in Japan by 2010. For the items, were obtained 1.29 million ton (MT) by telework, 1.1 MT by ITS, 0.53 MT by the reduction of paper consumption/rejection using LAN, and 0.5 MT by the alternation of movements for international business using Internet (reduction of overseas official trips). In addition, were obtained 0.36 MT by the energy saving with enhanced efficiency of office environment using advanced information communication such as building management information systems, 0.25 MT by the electronic publication and newspaper for the reduction of paper consumption/rejection using electronic media such as CD-ROM, and 0.03 MT by the remote education and at-home education systems. The trial calculations were conducted only for these seven systems, and only for the direct effects using these systems. Accordingly, the result is only a part of the whole. These systems promote to realize a society with reduced environmental loads. 2 tabs.

  9. Assessment of solid waste management systems in Ibadan North ...

    African Journals Online (AJOL)

    Solid waste management has been part of human activities right from time. Effort by the Oyo State Government in managing the collection and disposal of waste generated in Ibadan is seen in the provision of Skip bins at specific locations across the city. However, despite the provisions made by the government, ...

  10. Appearance of the application service and the application to the plant engineering. Engineering ASP stile EPSource.com; Apurikeshon sabisu no tojo to puranto enjiniaringu eno tekiyo. Enjiniaringu ASP saito EPSource.com

    Energy Technology Data Exchange (ETDEWEB)

    Nishi, Y.; Watanuma, Y. [Toyo Engineering Corp., Tokyo (Japan)

    2000-12-05

    In this paper, the change of soft wear usage and ESPsource.com of APS sight for plant engineering are introduced. Application service providers (APS) mean business enterprises disseminating application programs through network and distributing service of usage. This service has wide area because of many kinds of application and dissemination methods. Application relating plants is being widely such as production administration, maintenance, accounting system. The mechanism of APS is possible to distribute users supports as supporting systems. (NEDO)

  11. JUDGE project: a continental scientific drilling into plate subduction zone. 8. On archives of data and information of JUDGE; Shizumikomitai eno kussaku keikaku JUDGE. 8. Kussaku shishiryo archive ni kansuru kosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Miyazaki, T. [Geological Survey of Japan, Tsukuba (Japan); Tomizawa, N. [Schlumberger K.K., Tokyo (Japan)

    1997-04-28

    Systems for managing the data expected to be generated during the implementation of JUDGE (Japanese Ultra-deep Drilling and Geoscientific Experiments) Project are discussed from a viewpoint of archive construction. An archive system describes data in a computer system in compliance with grammar called a data model, and this enables efficient presentation and retrieval of information. Object data that is various in kind is classified by such categories as the class and the attribute. A data model means a format. The archive system conceptual design in this report consists of three layers for the user and four subspaces for data operation. This conceptual design is subjected to evaluation using actual data and existing systems, and it is found that the conceptual design is equipped with functions of storing and managing data and presenting information to the user. The AssetDB is an application that manages data of various types and functions (data relative to seismic exploration, logging, excavation, etc.). The function of the data processing, analytic subspaces is quite important in constructing archive systems. 7 refs., 3 figs., 3 tabs.

  12. Achievement report for fiscal 1997 (New Sunshine Project) on the development of superconductor power application technology. Research on a total system / investigative research on introduction effects; 1997 nendo chodendo denryoku oyo gijutsu kaihatsu (New Sunshine keikaku) seika hokokusho. Total system nado no kenkyu / donyu koka no chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This paper describes investigation and discussion on effects of introducing a superconductor power application technology. The generator sub-committee has compared features, technical problems and development expenses in the first and second proposals, but has not reached the final conclusion. The first proposal is targeted for early realization of 200-300 MW class generators with development cost of six to seven billion yen and a development term of seven years. These developments raise largely towards practical application the levels of material technologies, fabrication and processing technologies, and analysis and design technologies. The second proposal is intended of developing 200-300 MW class generators targeted for increased diameter, as a reduced size machine of a 600-MW generator being a future generator. It will take development cost of eight to nine billion yen with the period of nine years for the development. Majority of the technologies required for developing the future 600-MW class generator can be demonstrated. The AC superconductor device sub-committee has investigated design examples, clarified the specification requirements, and investigated and studied the development measures. The oxide superconductor sub-committee has investigated and studied making wires and conductors, and device application feasibility, but has not reached the stage of presenting specific research and development methods. The practical application strategy sub-committee has also not been able to present a collective measure because of difficulty of making future prospect on the electric power business. (NEDO)

  13. Research report for fiscal 1998. Development of advanced surface processing technology for methane-fueled aircraft engine members (Laser-aided advanced processing system technology); 1998 nendo chosa hokokusho. Methane nenryo kokukiyo engine buzai no kodo hyomen kako gijutsu kaihatsu (Laser oyo senshin kako system gijutsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    For the research and development of erosion-resistant abradable materials for the methane-fueled aircraft engine front section, a laser-aided surface reform technology was developed for Ti alloys and the like. In relation with the article 'Intermetallic Compound Coating Formation Technology,' an NiTi sprayed coating containing excess Ni solid solution was found to be quite high in resistance to erosion, and similar to Ti-6Al-4V in resistance to oxidation at 300 degrees C. Furthermore, an MCrAlY erosion-resistant coating was formed capable of resisting oxidation at temperatures higher than 1000 degrees C. In relation with the article 'Spraying Phenomenon Evaluation Technology,' studies were made of combustion synthesis reaction during plasma spraying and of the prediction of flight trajectories of different powders, for which optical fiber dichroic temperature measuring, 2-dimensional imaging, and LDV (laser Doppler velocimetry) were applied in combination. Concerning the spraying of intermetallic compound coatings, a temperature rise occurred when heating by laser was performed simultaneously with the laser-induced combustion synthesis reaction. In relation with the article 'Technology of Multiple Spraying on Curved Substrate,' it was found that the gas cooled method works effectively when spraying an erosion-resistant coating onto a thin Ti alloy made turbine blade. (NEDO)

  14. FY 1996 report on the results of the development of superconductor power application technology. Study of a total system, etc. (Investigational study of the introductory effect); 1996 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. Total system nado no kenkyu (donyu koka no chosa kenkyu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    Under the secondary interim assessment made in FY 1995 between the assessment committee of the energy/environment technology development sectional meeting of the Industrial Technology Deliberation Council and NEDO, in this project, the following were conducted in FY 1996: study of trial manufacture of superconductor motor model machine and R and D of element technology (conductor technology/refrigerator technology). The study was proceeded mainly with Super-GM, and as to the motor, the preparation for test on a part of the model machines was finished. Considering FY 1998 which is the final year of this project, the investigational study in FY 1996 aims at accumulating/arranging/analyzing the data which help judge how to proceed with the future R and D of superconductor power application technology continuously based on the secondary interim assessment. For superconductor motor and various superconductor power appliance, economical/technical feasibilities and methods of R and D and surveys of trends in Japan and abroad for the R and D were made important items. Further, 'R and D of the basement of superconductor power application' proposed in the secondary interim assessment (R and D of the combination of elements which become the basement of equipment technology over a stage of parallel R and D of element technology of conductor, etc.) targeted the presentation of concrete details. (NEDO)

  15. Family of highly accurate superstable time integration algorithms of implicit two step and their application to solution of the Navier-Stokes equations; Inkyoku nidan katei no choantei koseido jikan sekibunho to Navier-Stokes hoteishiki kaiho eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Y.; Yuan, W. [Tokyo Institute of Technology, Tokyo (Japan); Uochi, S. [East Japan Railway Co., Tokyo (Japan)

    1998-04-21

    A direct time integration is proposed of implicit two steps for the system which is the object of analysis to result in a one-step simultaneous ordinary differential equation relative to time, in which the accuracy of evaluation of an extremely large eigenvalue is radically improved. This is an implicit solution, and the structure of the coefficient matrix is structurally the same as that of the original system, and the advantage in the memorizing program is not lost. In the two-step solution, the coefficient matrix in the last half of integral calculation turns out to be the same as that in the first half, and calculation in the last half can proceed by only forward and backward operations or substitution. It is a self-starting type, and is equipped with a general-purpose feature in that it can exhibit different characteristics dependent upon selected parameters. The improved version is applied to the Navier-Stokes finite element equation for the construction of a solution, and this verifies that it is practically effective. In nonlinear analysis in particular, the reliability of solution greatly influences the evaluation of the nonlinear term, and this often makes analysis difficult. The integration proposed here will greatly contribute to the perfection of analytic systems that are to deal with nonlinear phenomena. 26 refs., 15 figs., 1 tab.

  16. Study on the ultra-high speed LAN corresponding to the ultra-high speed IP network of Kansai Electric Power Co.; LAN no chokosokuka ni kansuru kenkyu. Tosha IP network no chokosokuka eno taio hoho

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, R. [Kansai Electaric Power Co. Inc., Osaka (Japan)

    2000-04-01

    This study was made on survey and evaluation of high-speed network technology and routing protocol based on IP (Internet protocol) network. This paper outlines the evaluation result, and explains the performance verification test result on the routing protocol OSPF (open shortest pass fast) used for the IP network of Kansai Electric Power Co. High-speed LAN using Giga-Bit Ethernet instead of Ethernet was confirmed to be more profitable than ATM-LAN from the viewpoint of cost reduction, operation control, and affinity with the existing IP network. POS (packet over synchronous digital hierarchy) system was adopted as high-speed WAN (wide area network) from the viewpoint of cost reduction, and affinity with the existing IP network. OSPF system was adopted as routing protocol for the next-generation IP network because of its affinity with loop type topology and shorter circuit switching time. The verification test result of OSPF showed such features as short circuit switching time, optimization of a communication route by selecting the widest-band route, and flexible modification of topology. (NEDO)

  17. Fiscal 1997 report on the feasibility survey of Japan`s cooperation into energy/environment related fields in Asian countries; 1997 nendo chosa hokokusho (Asia shokoku ni okeru energy kankyo kanren bun`ya eno kyoryoku kanosei chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    A survey was made to study possibilities of Japan`s cooperation in tackling energy/environment problems in Asian countries. In 1995 the GNP growth rate was 2.6% in the world, while it was 7.9% in Asia showing a rapid growth. With the accelerating industrialization and urbanization, energy demand is now over three times as much as that in the 1980s. This means increases in waste and environmental pollution. Especially, the use of firewood in non-electrified areas is a serious problem the same as the slash-and-burn farming and deforestation. Also relating to the production of electric power, it is inevitable to choose hydroelectric power and renewable energy in addition to fossil fuels. Therefore, the necessity is heightening of various technologies for it and environmental protection technologies such as coal cleaning. Local areas, where the population density is extremely low and the electrification cost is high, became victims of elecrification. There the power system should be shifted from the central supply system to the local distributed one. For it, it is necessary to recognize an importance of the distributed type renewable energy which is friendly to the environment. The paper outlined the state of each country and energy by policy and environment. 10 figs., 83 tabs.

  18. Insulation recovery characteristics after arc interruption and its application for high speed reclosing of UHV A. C transmission lines. UHV koryu sodensen ni okeru arc shokogo no zetsuen kaifuku tokusei to kosokudo saihero hoshiki eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Goda, Yutaka; Matsuda, Shoji; Inaba, Tsuginori.

    1988-11-01

    An application of high speed reclosing for UHV a.c transmission lines is expected to secure high reliability of transmission system. In order to choose dead times of high speed reclosing property, it is necessary to clarify forced extinction characteristics of secondary arc and insulation recovery characteristics after arc interruption. The authors carried out many tests about these. This reports shows summarization of the results of these test. The authors applied a statistical method to measure insulation recovery characteristics after arc interruption, and calculated some dead times under some assumption. From the results of the tests, the authors considered that the technique of high speed grounding switch is effective for realizing high speed reclosing. They showed the results of calculation to choose dead times of high speed reclosing. 21 refs., 35 figs., 10 tabs.

  19. Regulation of the sympathetic nervous system by nitric oxide and oxidative stress in the rostral ventrolateral medulla: 2012 Academic Conference Award from the Japanese Society of Hypertension.

    Science.gov (United States)

    Kishi, Takuya

    2013-10-01

    Sympathoexcitation has an important role in the pathogenesis of hypertension. Previous studies have demonstrated that nitric oxide (NO) and/or oxidative stress in the brain are important for the regulation of the sympathetic nervous system. We have investigated the role of NO derived from an overexpression of endothelial NO synthase (eNOS) or oxidative stress in the rostral ventrolateral medulla (RVLM), which is known as a vasomotor center in the brainstem, on the regulation of the sympathetic nervous system. Our results indicated that NO derived from an overexpression of eNOS in the RVLM caused sympathoinhibition via an increase in γ-amino butyric acid and that angiotensin II type 1 receptor (AT1R)-induced oxidative stress in the RVLM caused sympathoexcitation. We also demonstrated that oxidative stress in the RVLM caused sympathoexcitation via interactions with NO, effects on the signal transduction or apoptosis of the astrocytes. Furthermore, several orally administered AT1R blockers have been found to cause sympathoinhibition via a reduction in oxidative stress through the blockade of AT1R in the RVLM of hypertensive rats. In conclusion, our studies suggest that the increase in AT1R-induced oxidative stress and/or the decrease in NO in the RVLM mainly cause sympathoexcitation in hypertension.

  20. SYSTEM

    Directory of Open Access Journals (Sweden)

    K. Swarnalatha

    2013-01-01

    Full Text Available Risk analysis of urban aquatic systems due to heavy metals turns significant due to their peculiar properties viz. persis tence, non-degradab ility, toxicity, and accumulation. Akkulam Veli (AV, an urba n tropical lake in south India is subjected to various environmental stresses due to multiple waste discharge, sand mining, developmental activities, tour ism related activitie s etc. Hence, a comprehensive approach is adopted for risk assessment using modified degree of contamination factor, toxicity units based on numerical sediment quality guidelines (SQGs, and potentialecological risk indices. The study revealed the presence of toxic metals such as Cr, C d, Pb and As and the lake is rated under ‘low ecological risk’ category.

  1. system

    Science.gov (United States)

    Garcilazo, H.; Valcarce, A.; Vijande, J.

    2017-07-01

    Using local central Yukawa-type Malfliet-Tjon interactions reproducing the low-energy parameters and phase shifts of the nn system, and the latest updates of the nΛ and ΛΛ Nijmegen ESC08c potentials, we study the possible existence of a bound state. Our results indicate that the is unbound, being just above threshold. We discuss the role played by the 1 S 0 nn repulsive term of the Yukawa-type Malfliet-Tjon interaction. Supported by COFAA-IPN (México), Ministerio de Economía, Industria y Competitividad and EU FEDER (FPA2013-47443, FPA2015-69714-REDT, FPA2016-77177), Junta de Castilla y León (SA041U16) and Generalitat Valenciana PrometeoII/2014/066

  2. Mathematical model for predicting transformation of high carbon steel during cooling and its application to on-line temperature control in hot strip mill; Kotansoko no hentai yosoku model no kaihatsu to sono jikki eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Suehiro, M.; Oda, T.; Senuma, T.; Konishi, S. [Nippon Steel Corp., Tokyo (Japan)

    1995-11-06

    A mathematical model for predicting transformation of high carbon steel during cooling (transformation from austenite to pearlite, ferrite, and bainite) has been developed. The basic equation for this model is the Cahn`s transformation progress behavior indicating equation, from which an equation was introduced that represents transformation velocity for the case of generation and growth of nuclei and for the case of site saturation. Using these equations makes it possible to calculate transformation in arbitrary cooling processes. In addition, a prediction model for hot processing transformation that expresses the influence of the hot processing on transformation was coupled with the transformation equation to improve the accuracy of transformation prediction. Rise in steel plate temperature that takes place because of generation of transformation latent heat during cooling was calculated by using a two-dimensional heat conduction equation. Off-line applications of this model include prediction of steel plate temperatures on a hot-run table, improvement in productivity by increasing plate passing speed in a continuous hot rolling process, and correction of variation in finishing rolling temperature. On-line applications include controls in hot-run water injection facilities, and automation of an on-line control system. 13 refs., 18 figs., 1 tab.

  3. African Journal for the Psychological Study of Social Issues - Vol 19 ...

    African Journals Online (AJOL)

    educational secondary school students in Ibadan, South West Nigeria · EMAIL FULL ... Influence of core self-evaluation and reward system on organisational commitment of local government employees in Oyo State, Nigeria · EMAIL FULL TEXT ...

  4. An assessment of the forest regeneration potential of the taungya ...

    African Journals Online (AJOL)

    -increasing population challenges is imperative to her sustainable development. Two methods of artificial forest regeneration practiced in Oyo State, Nigeria to meet these challenges are direct planting and taungya systems. This paper reports ...

  5. Virtual law library as a veritable tool for justice delivery system in ...

    African Journals Online (AJOL)

    The study investigated the influence of virtual library use in legal practice and its contributions to quick administration of justice. The study adopted descriptive survey research design, and simple random sampling technique was used in selecting thirty (30) Law library personnel across the state, but all the 26 judges of Oyo ...

  6. Chlorine gas exposure causes systemic endothelial dysfunction by inhibiting endothelial nitric oxide synthase-dependent signaling.

    Science.gov (United States)

    Honavar, Jaideep; Samal, Andrey A; Bradley, Kelley M; Brandon, Angela; Balanay, Joann; Squadrito, Giuseppe L; MohanKumar, Krishnan; Maheshwari, Akhil; Postlethwait, Edward M; Matalon, Sadis; Patel, Rakesh P

    2011-08-01

    Chlorine gas (Cl(2)) exposure during accidents or in the military setting results primarily in injury to the lungs. However, the potential for Cl(2) exposure to promote injury to the systemic vasculature leading to compromised vascular function has not been studied. We hypothesized that Cl(2) promotes extrapulmonary endothelial dysfunction characterized by a loss of endothelial nitric oxide synthase (eNOS)-derived signaling. Male Sprague Dawley rats were exposed to Cl(2) for 30 minutes, and eNOS-dependent vasodilation of aorta as a function of Cl(2) dose (0-400 ppm) and time after exposure (0-48 h) were determined. Exposure to Cl(2) (250-400 ppm) significantly inhibited eNOS-dependent vasodilation (stimulated by acetycholine) at 24 to 48 hours after exposure without affecting constriction responses to phenylephrine or vasodilation responses to an NO donor, suggesting decreased NO formation. Consistent with this hypothesis, eNOS protein expression was significantly decreased (∼ 60%) in aorta isolated from Cl(2)-exposed versus air-exposed rats. Moreover, inducible nitric oxide synthase (iNOS) mRNA was up-regulated in circulating leukocytes and aorta isolated 24 hours after Cl(2) exposure, suggesting stimulation of inflammation in the systemic vasculature. Despite decreased eNOS expression and activity, no changes in mean arterial blood pressure were observed. However, injection of 1400W, a selective inhibitor of iNOS, increased mean arterial blood pressure only in Cl(2)-exposed animals, suggesting that iNOS-derived NO compensates for decreased eNOS-derived NO. These results highlight the potential for Cl(2) exposure to promote postexposure systemic endothelial dysfunction via disruption of vascular NO homeostasis mechanisms.

  7. Chlorine Gas Exposure Causes Systemic Endothelial Dysfunction by Inhibiting Endothelial Nitric Oxide Synthase–Dependent Signaling

    Science.gov (United States)

    Honavar, Jaideep; Samal, Andrey A.; Bradley, Kelley M.; Brandon, Angela; Balanay, Joann; Squadrito, Giuseppe L.; MohanKumar, Krishnan; Maheshwari, Akhil; Postlethwait, Edward M.; Matalon, Sadis; Patel, Rakesh P.

    2011-01-01

    Chlorine gas (Cl2) exposure during accidents or in the military setting results primarily in injury to the lungs. However, the potential for Cl2 exposure to promote injury to the systemic vasculature leading to compromised vascular function has not been studied. We hypothesized that Cl2 promotes extrapulmonary endothelial dysfunction characterized by a loss of endothelial nitric oxide synthase (eNOS)-derived signaling. Male Sprague Dawley rats were exposed to Cl2 for 30 minutes, and eNOS-dependent vasodilation of aorta as a function of Cl2 dose (0–400 ppm) and time after exposure (0–48 h) were determined. Exposure to Cl2 (250–400 ppm) significantly inhibited eNOS-dependent vasodilation (stimulated by acetycholine) at 24 to 48 hours after exposure without affecting constriction responses to phenylephrine or vasodilation responses to an NO donor, suggesting decreased NO formation. Consistent with this hypothesis, eNOS protein expression was significantly decreased (∼ 60%) in aorta isolated from Cl2–exposed versus air-exposed rats. Moreover, inducible nitric oxide synthase (iNOS) mRNA was up-regulated in circulating leukocytes and aorta isolated 24 hours after Cl2 exposure, suggesting stimulation of inflammation in the systemic vasculature. Despite decreased eNOS expression and activity, no changes in mean arterial blood pressure were observed. However, injection of 1400W, a selective inhibitor of iNOS, increased mean arterial blood pressure only in Cl2–exposed animals, suggesting that iNOS-derived NO compensates for decreased eNOS-derived NO. These results highlight the potential for Cl2 exposure to promote postexposure systemic endothelial dysfunction via disruption of vascular NO homeostasis mechanisms. PMID:21131444

  8. Kaval-Ants ja Vanapagan : [luuletused] / Eno Raud

    Index Scriptorium Estoniae

    Raud, Eno, 1928-1996

    2001-01-01

    Sisu : Kaval-Ants ja Vanapagan ; Aida ust valvamas ; Vanapaar ; Pada ja katel ; Voki laul karule ; Laisk laiskloom ; Soovitus ; Käbi käbinäbi ; Mitmesugused lapsed ; Päevavaras ; Vaba rabakana ; Vetesell ; Padakonna konnavada ; Mis saiast sai? ; Siili nohu; Lõunatuul õunapuul ; Käo kukkumine ; Vankrisõit

  9. Erakondade valimisraha tekitab kahtlusi / Eno-Gerrit Link, Riina Kuru

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2002-01-01

    Reformierakonna poliitilised oponendid Pärnus kahtlustavad, et 1999. a. kohalike omavalitsuste valimiste ajal oli Reformierakonna sponsoriks mõni firma, näiteks AS Pärnu Sadam. Tabel: Raha erakonnale. Kommenteerivad Väino Linde, Liia Hänni. Parlamendisaadik

  10. The effects of exercise training and caloric restriction on the cardiac oxytocin natriuretic peptide system in the diabetic mouse

    Directory of Open Access Journals (Sweden)

    Broderick TL

    2017-01-01

    Full Text Available Tom L Broderick,1 Marek Jankowski,2 Jolanta Gutkowska2 1Department of Physiology, Laboratory of Diabetes and Exercise Metabolism, Midwestern University, Glendale, AZ, USA; 2Department of Medicine, Laboratory of Cardiovascular Biochemistry, Centre Hospitalier de l‘Université de Montréal-Hôtel-Dieu, Montréal, QC, Canada Background: Regular exercise training (ET and caloric restriction (CR are the frontline strategies in the treatment of type 2 diabetes mellitus with the aim at reducing cardiometabolic risk. ET and CR improve body weight and glycemic control, and experimental studies indicate that these paradigms afford cardioprotection. In this study, the effects of combined ET and CR on the cardioprotective oxytocin (OT–natriuretic peptide (NP system were determined in the db/db mouse, a model of type 2 diabetes associated with insulin resistance, hyperglycemia, and obesity. Methods: Five-week-old male db/db mice were assigned to the following groups: sedentary, ET, and ET + CR. Nonobese heterozygote littermates served as controls. ET was performed on a treadmill at moderate intensity, and CR was induced by reducing food intake by 30% of that consumed by sedentary db/db mice for a period of 8 weeks. Results: After 8 weeks, only ET + CR, but not ET, slightly improved body weight compared to sedentary db/db mice. Regardless of the treatment, db/db mice remained hyperglycemic. Hearts from db/db mice demonstrated reduced expression of genes linked to the cardiac OT–NP system. In fact, compared to control mice, mRNA expression of GATA binding protein 4 (GATA4, OT receptor, OT, brain NP, NP receptor type C, and endothelial nitric oxide synthase (eNOS was decreased in hearts from sedentary db/db mice. Both ET alone and ET + CR increased the mRNA expression of GATA4 compared to sedentary db/db mice. Only ET combined with CR produced increased eNOS mRNA and protein expression. Conclusion: Our data indicate that enhancement of eNOS by combined

  11. Localisation of the nitric oxide (NO)/cGMP-pathway in the vestibular system of guinea pigs.

    Science.gov (United States)

    Hess, A; Bloch, W; Su, J; Stennert, E; Addicks, K; Michel, O

    1998-07-31

    The exact distribution of nitric oxide-synthases (NOS) in the vestibular system has not been described satisfying yet. Immunostaining, using specific antibodies to the three known NOS-isoforms, to cyclic guanosine monophosphate (cGMP) and soluble guanylyl-cyclase (sGC), the second messenger system of nitric oxide (NO), was performed on paraffin sections of temporal bone from guinea pigs. eNOS could be detected in vestibular ganglion cells and in nerve fibres, including the calyces, surrounding the type 1 hair cells (HC). bNOS was found in the sensory epithelium, ganglion cells and in bone, while iNOS could not be found. NOS-detection was accompanied by reactivity to sGC and to cGMP. This finding implies that b- and eNOS-generated NO is involved in regulative processes in neurotransmission and regulation of blood flow.

  12. Report on results for fiscal 1997 on development of superconducting electric power application technology. Pt. 2. R and D of superconducting wire, R and D of superconducting generator, studies on total system, R and D of refrigeration system, and verification test; 1997 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total system no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    This report refers, continuously to the Part 1, to the in-situ verification test of the slow-response type model machine rotor and to the review of the conceptual design of the pilot machine. On the basis of the R and D results of various element technology/partial models obtained before the previous fiscal year, the design, manufacturing and factory test had been conducted for a 70,000kW class slow-response type model machine rotor. This year, an in-situ verification test was performed to complete the test of all planned test items. Using the technological results obtained in the design, manufacturing and test of the 70,000kW class model machine, the conceptual design is being reviewed of the 200,000kW class pilot machine. In the aspect of the functional design, accurate grasping of the thermal load is essential for the purpose of attaining a large capacity for a superconducting generator, as a part of which a thermal load analytical method was planned to be established for a torque tube heat exchanger. The reasonableness of the analysis was verified through a comparison with the factory test result of the 70,000kW class slow-response type rotor, indicating good agreement between the calculation result and the actual measurement, and enabling the result to be obtained that explains dependency of the thermal load on the number of revolution. (NEDO)

  13. Report on results for fiscal 1996 on development of superconducting electric power application technology. Pt. 2. R and D of superconducting wire, R and D of superconducting generator, studies on total system, R and D of refrigeration system, and verification test; 1996 nendo chodendo denryoku oyo gijutsu kaihatsu seika hokokusho. 2. Chodendo senzai no kenkyu kaihatsu, chodendo hatsudenki no kenkyu kaihatsu, total system no kenkyu, reito system no kenkyu kaihatsu, jissho shiken

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    In fiscal 1996, the initial cooling test and rotary excitation test were conducted for the 70,000kW slow-response type model machine, with the basic characteristics of the rotor verified such as the cooling characteristics, shaft vibration characteristics, mechanical strength, and superconductivity stability, and with the results of the studies explained. The rotor of the 70,000kW slow-response type model was installed in the test site, and the field test was carried out in 1997. For this purpose, research test was performed by the element technology of the model machine and the partial model. The technological development items, trial manufacture and test items were field winding, multi cylindrical rotor and damper. Upon the test results using the element and partial model, the design and manufacturing were implemented of the superconducting generator of a slow-response type excitation method with the output of 70,000kW class. Performed were the basic characteristic test, long-term operation test and severity test on the assumption of abnormality, verifying the characteristics and reliability of the generator as well as obtaining data concerning an excessive electromagnetic force and heat generation. From the test data and the analysis/evaluation results obtained on the model machine, the 200,000kW class pilot machine was reviewed in the conceptual design. (NEDO)

  14. Age-related changes of the nitric oxide system in the rat brain.

    Science.gov (United States)

    Siles, Eva; Martínez-Lara, Esther; Cañuelo, Ana; Sánchez, Marta; Hernández, Raquel; López-Ramos, Juan Carlos; Del Moral, María Luisa; Esteban, Francisco José; Blanco, Santos; Pedrosa, Juan Angel; Rodrigo, José; Peinado, María Angeles

    2002-11-29

    This work examines the age-related changes of the NO pathway in the central nervous system (CNS), analyzing nitric oxide synthase (NOS) isoform expression, the level of nitrotyrosine-modified proteins, and the NOS activity in the cerebral cortex, decorticated brain (basal ganglia, thalamus, hypothalamus, tegtum and tegmentum) and cerebellum of young, adult and aged rats. Our data demonstrate that the different NOS isoforms are not uniformly expressed across the CNS. In this sense, the nNOS and eNOS isoenzymes are expressed mainly in the cerebellum and decorticated brain, respectively, while the iNOS isoenzyme shows the highest level in cerebellum. Concerning age, in the cerebral cortex nNOS significantly increased its expression only in adult animals; meanwhile, in the cerebellum the eNOS expression decreased whereas iNOS increased in adult and aged rats. No age-related changes in any isoform were found in decorticated brain. NOS activity, determined by nitrate plus nitrite quantification, registered the highest levels in the cerebellum, where the significant increase detected with aging was probably related to iNOS activity. The number of nitrotyrosine-modified immunoreactive bands differed among regions; thus, the highest number was detected in the decorticated brain while the cerebellum showed the least number of bands. Finally, bulk protein nitration increased in cerebral cortex only in adult animal. No changes were found in the decorticated brain, and the decrease detected in the cerebellum of aged animals was not significant. According to these results, the NO pathway is differently modified with age in the three CNS regions analyzed. Copyright 2002 Elsevier Science B.V.

  15. Floristic indicators of tropical landuse systems: Evidence from mining areas in Southwestern Nigeria

    Directory of Open Access Journals (Sweden)

    Sehinde Akinbiola

    2016-07-01

    Full Text Available Most forests in the tropics have not only been reduced in size but have also experienced forest degradation. The delicate balance of the components of the forest ecosystem has been altered largely by the landuse systems which have resulted in the disappearance of some species and the introduction of new ones. In order to understand the influence of human disturbance caused by artisanal mining on plant biodiversity and the physical environment, this study assessed changes in vegetation characteristics using plant functional groups, such as invasive, pioneer and understory species. The study was conducted at two sites in two Southwestern states of Nigeria, Itagunmodi in Atakumosa West Local Government Area of Osun State and Olode in Oluyole Local Government Area of Oyo State. Complete enumerations of woody, non-woody and herbaceous plant species were carried out in 20 m by 20 m plots selected using stratified random sampling as representative of landuse classes: freshly mined (6 months and a control plot (secondary forest. The results showed that the control plot in Itagunmodi had undergone degradation as indicated by the presence of Alchornea laxiflora, Geophilia obvallata, Musa sapientum,Elaeis guineensis and Keetia hispida. However, if left undisturbed, it has the potential of regeneration back to its original state because of its woody tree species composition and lianas (Triclisia gilletii. In addition the forest soils in the mines had been exposed to direct insolation for a long period, and the soil structure and texture have been affected, consequently altering the viability of the seed bank. Poaceae and Papilionoideae were the most common in the freshly mined plots while invasive species were the most common in abandoned mined plots. In conclusion, the floristic composition in the mining sites has been altered and there was no clear evidence that regrowth would bring restoration of the lost ecosystem services.

  16. Computational modeling of shear forces and experimental validation of endothelial cell responses in an orbital well shaker system.

    Science.gov (United States)

    Filipovic, Nenad; Ghimire, Kedar; Saveljic, Igor; Milosevic, Zarko; Ruegg, Curzio

    2016-01-01

    Vascular endothelial cells are continuously exposed to hemodynamic shear stress. Intensity and type of shear stress are highly relevant to vascular physiology and pathology. Here, we modeled shear stress distribution in a tissue culture well (R = 17.5 mm, fill volume 2 ml) under orbital translation using computational fluid dynamics with the finite element method. Free surface distribution, wall shear stress, inclination angle, drag force, and oscillatory index on the bottom surface were modeled. Obtained results predict nonuniform shear stress distribution during cycle, with higher oscillatory shear index, higher drag force values, higher circular component, and larger inclination angle of the shear stress at the periphery of the well compared with the center of the well. The oscillatory index, inclination angle, and drag force are new quantitative parameters modeled in this system, which provide a better understanding of the hydrodynamic conditions experienced and reflect the pulsatile character of blood flow in vivo. Validation experiments revealed that endothelial cells at the well periphery aligned under flow and increased Kruppel-like Factor 4 (KLF-4), cyclooxygenase-2 (COX-2) expression and endothelial nitric oxide synthase (eNOS) phosphorylation. In contrast, endothelial cells at the center of the well did not show clear directional alignment, did not induce the expression of KLF-4 and COX-2 nor increased eNOS phosphorylation. In conclusion, this improved computational modeling predicts that the orbital shaker model generates different hydrodynamic conditions at the periphery versus the center of the well eliciting divergent endothelial cell responses. The possibility of generating different hydrodynamic conditions in the same well makes this model highly attractive to study responses of distinct regions of the same endothelial monolayer to different types of shear stresses thereby better reflecting in vivo conditions.

  17. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1994. 2. Main issue (Part 3 for research and development of a simulated environment presenting technology); 1994 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu. 2. Honronhen (mogi kankyo teiji gijutsu no kenkyu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    This paper describes the research and development of (1) a composite acoustic environment presenting technology, (2) a radiation model to serve for enhancing working environments, and (3) high-accuracy recognition of actual senses and visual sense. With regard to the subject (1), fabrication was made on the composite acoustic environment presenting device and its control system, a psychology experimenting system and a measuring system. In addition, a subjectivity evaluation experiment was carried out in an environment in which air propagating sound, solid propagating sound and low-frequency sound are compounded. With regard to the subject (2), the completed artificial sunlight radiation device model was given tests on reproducibility and spectral change due to lamp using time. In addition, this model was performed of characteristics investigation in radiation environment, such as on physical amount of hot heat environment due to availability of sunlight from windows when operated in an artificial environment experimenting room, and on the difference between the case when operated mainly using halogen lamps and the case when operated under artificial sunlight simulated radiation. With respect to the subject (3), element models required in electric power plant operation environment were realized as a class library by introducing the object orientation technology. These models were used to structure a system to discuss operability in the power plant operation environment in the virtual-real world. (NEDO)

  18. Statins and oxidative stress in the cardiovascular system.

    Science.gov (United States)

    Margaritis, Marios; Sanna, Fabio; Antoniades, Charalambos

    2017-09-26

    Statins are widely established as an important class of medications for primary and secondary prevention of cardiovascular disease. In addition to their lipid-lowering effects, mounting evidence suggests that statins exhibit non-lipid-lowering mediated effects in the cardiovascular system. These so called "pleiotropic" effects are partly due to antioxidant properties of statins. These are mediated by inhibition of the mevalonate pathway, which interferes with small GTP-ase protein prenylation. This, in turn, leads to anti-oxidant effects of statins via a plethora of mechanisms. Statins prevent the activation of the pro-oxidant enzyme NADPH-oxidase by interfering with Rac1 activation and translocation to the membrane, as well as reducing expression of crucial subunits of NADPH-oxidase. Statins also enhance the expression, enzymatic activity and coupling of endothelial nitric oxide synthase (eNOS), through mevalonate-dependent effects. The net result is a restoration of the redox balance in the cardiovascular system, with subsequent anti-atherosclerotic and cardioprotective effects. While the evidence from basic science studies and animal models is strong, more clinical trials are required to establish the relevance of these pleiotropic effects to human cardiovascular disease and potentially lead to expanded indications for statin treatment or alternative therapeutic strategies. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  19. Aspirin and lipid mediators in the cardiovascular system.

    Science.gov (United States)

    Schrör, Karsten; Rauch, Bernhard H

    2015-09-01

    Aspirin is an unique compound because it bears two active moieties within one and the same molecule: a reactive acetyl group and the salicylate metabolite. Salicylate has some effects similar to aspirin, however only at higher concentrations, usually in the millimolar range, which are not obtained at conventional antiplatelet aspirin doses of 100-300 mg/day. Pharmacological actions of aspirin in the cardiovascular system at these doses are largely if not entirely due to target structure acetylation. Several classes of lipid mediators become affected: Best known is the cyclooxygenase-1 (COX-1) in platelets with subsequent inhibition of thromboxane and, possibly, thrombin formation. By this action, aspirin also inhibits paracrine thromboxane functions on other lipid mediators, such as the platelet storage product sphingosine-1-phosphate (S1P), an inflammatory mediator. Acetylation of COX-2 allows for generation of 15-(R)HETE and subsequent formation of "aspirin-triggered lipoxin" (ATL) by interaction with white cell lipoxygenases. In the cardiovascular system, aspirin also acetylates eNOS with subsequent upregulation of NO formation and enhanced expression of the antioxidans heme-oxygenase-1. This action is possibly also COX-2/ATL mediated. Many more acetylation targets have been identified in live cells by quantitative acid-cleavable activity-based protein profiling and might result in discovery of even more aspirin targets in the near future. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Toward a reduced-wire readout system for ultrasound imaging.

    Science.gov (United States)

    Lim, Jaemyung; Arkan, Evren F; Degertekin, F Levent; Ghovanloo, Maysam

    2014-01-01

    We present a system-on-a-chip (SoC) for use in high-frequency capacitive micromachined ultrasonic transducer (CMUT) imaging systems. This SoC consists of trans-impedance amplifiers (TIA), delay locked loop (DLL) based clock multiplier, quadrature sampler, and pulse width modulator (PWM). The SoC down converts RF echo signal to baseband by quadrature sampling which facilitates modulation. To send data through a 1.6 m wire in the catheter which has limited bandwidth and is vulnerable to noise, the SoC creates a pseudo-digital PWM signal which can be used for back telemetry or wireless readout of the RF data. In this implementation, using a 0.35-μm std. CMOS process, the TIA and single-to-differential (STD) converter had 45 MHz bandwidth, the quadrature sampler had 10.1 dB conversion gain, and the PWM had 5-bit ENoB. Preliminary results verified front-end functionality, and the power consumption of a TIA, STD, quadrature sampler, PWM, and clock multiplier was 26 mW from a 3 V supply.

  1. Increased expression of the renin-angiotensin system and mast cell density but not of angiotensin-converting enzyme II in late stages of human heart failure.

    Science.gov (United States)

    Batlle, Montserrat; Roig, Eulàlia; Perez-Villa, Fèlix; Lario, Sergio; Cejudo-Martin, Pilar; García-Pras, Ester; Ortiz, José; Roqué, Mercé; Orús, Josefina; Rigol, Montserrat; Heras, Magdalena; Ramírez, José; Jimenez, Wladimiro

    2006-09-01

    The activation of the renin-angiotensin system (RAS) contributes to the progression of left ventricular dysfunction. A novel human homologue of the angiotensin-converting enzyme (ACE), named ACE2, has been described but its role in human heart failure (HF) has not been elucidated. Besides, there is controversy as to whether the major angiotensin II-forming-activity in heart is ACE or chymase released from mast cells. Furthermore, long-term blockade of nitric oxide (NO) synthesis has been shown to increase ACE activity. To assess the locally activated vasoactive mediators that may contribute to the ventricular deterioration process, we sought to simultaneously analyze their expression in failing hearts. We analyzed left ventricular biopsies from 30 patients with heart failure undergoing heart transplantation and 12 organ donors. The mRNA levels of ACE, ACE2, chymase and endothelial nitric oxide synthase (eNOS), were quantified by real-time polymerase chain reaction and mast cell density was assessed by immunohistochemistry. The mRNA levels of the atrial natriuretic peptide (ANP) and the brain natriuretic peptide (BNP) were also quantified as controls. There was higher ACE and chymase mRNA expression and mast cell density in failing than in control myocardium and no changes in ACE2 expression were detected. eNOS mRNA levels were lower in failing hearts. Both ANP and BNP expression were higher in pathological than in control samples. These data document a decompensation of vasoactive systems that may contribute to the progressive impairment of the myocardial function in HF. On the other hand, ACE2 mRNA expression is not altered in human end-stage HF.

  2. FY 1991 report on the results of the contract R and D of the human sense measuring application technology. 4. R and D of correlation/evaluation technology (1); 1991 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu seika hokokusho. 4. Sokan hyoka gijutsu no kenkyu kaihatsu (1)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-03-01

    As a part of the R and D of correlation/evaluation technology, the following R and D were conducted in this paper: (1) high accuracy real sense visual recognition; (2) working environment at nuclear power plant with human sense and affinity; (3) amenity correlation technology in the combined environment mainly of thermal environment. In (1), the following seven items were studied: software for high accuracy real sense visual recognition; software for detecting contact of human movement with object, etc.; improvement of performance of speed of CG image indicator; harmony between image expression and video presentation; software technology for high speed modeling; measuring system under the virtual reality; production of a set of prototype system. In (2), as the environment of nuclear power plant site, visual environment of centralized control room; audio-environment of machinery room, etc., and radio-environment; environment where people have to put on special working ware, etc. In (3), development of a system to examine correlations among external stimulus environment/physiological effect/sensory quantity based on physiological reaction models; development of technology to quantitatively estimate amenity in the combined environment mainly of thermal environment. (NEDO)

  3. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1993. 4. Research and development of correlation and evaluation technology (Part 2); Ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu. 4. Sokan hyoka gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    Developments were performed during fiscal 1993 on a system to investigate correlation among external stimulating environment, physiological effects and sense volume based on a physiological reaction model. The development works included improvement of the model, discussions on a method to estimate parameters inside living organisms, and a method to collect subjectivity volume data. Prototypes thereof were fabricated, evaluated, and experimented, where nearly complete achievement of the targeted functions was verified. In developing a technology to evaluate quantitatively the comfort of composite environments composed mainly of warm heat environment, discussions were given on an integration evaluating expression in order not to make handling the environmental physical volume too complex. The expression models the evaluation of governing sense volume in individual environments of warm heat, light beam, and sound, and calculates the composite environment evaluation indexes by using the evaluation indexes obtained from the modeling as the descriptive variables. In developing the space-type human interface adapted to human senses, a computer system to realize the interface and the platform for function mounting were developed. The development items were broken down respectively on the basic technologies for functional aspect and surveillance and control function mounting, and the software systems. The human comfort meter achieved the basic design target. (NEDO)

  4. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1993. 4. Research and development of correlation and evaluation technology (Part 1); Ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu. 4. Sokan hyoka gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    The achievements during fiscal 1993 of research and development on a method to evaluate warm heat comfort by living scenes of workers by using a human body thermal model included completion of a human thermal model introduced with physiological knowledge such as artery-vein anastomosed (AVA) blood vessels and two-system blood circulation net. The effectiveness of the model was experimented and verified. It was made possible to estimate the reversal phenomenon occurring in hot heat environment, in which skin temperatures at peripheral body parts rise higher than those in the body trunk part, and skin temperature distribution. In the correlation study to estimate statistically the sense volume from skin temperatures, it was possible to estimate significantly different sense volumes from face skin temperatures, but the estimation accuracy was low because of such factors as difference in individuals. What is important in enhancing the accuracy is to identify ecological reactions of human being caused from change in the sense volume, select effective parameters, and study the mechanisms. In order to develop stress relaxing scents, discussions were given on degree of subjectivity stress by question papers and cortisol concentration in saliva as the parameters. An experimental system was developed to measure the effects of the scents to relax work stresses. A screening method was developed, whereas effects of 15 kinds of scent materials were investigated. (NEDO)

  5. Report on achievements of research and development of a technology to apply human senses to measurements in fiscal 1992. 4. Research and development of a correlation and evaluation technology (Part 1); 1992 nendo ningen kankaku keisoku oyo gijutsu no kenkyu kaihatsu. 4. Sokan hyoka gijutsu no kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-03-01

    This paper describes the correlation and evaluation technology, extracted from the achievements in the development of a technology to apply human senses to measurements in fiscal 1992. In evaluating the warm heat comfort by living scenes of workers according to the human body thermal model, the research environment was improved by expanding the systems to measure physiological volume and the warm heat environment. Findings were acquired on the relationship between body fat rate and skin temperature, and actions of artery-vein anastomosed (AVA) blood vessels. These findings were incorporated into the research to elevate the levels of applicability of the human body thermal model. In estimating the sense volume by using skin temperatures, physiological volume being the factor of variation in face skin temperatures was elucidated, and a system to measure these variations was structured. In order to acquire the physiological volume being the factor of variation in face skin temperatures, brain wave topography measurement and {alpha} frequency fluctuation and arousal evaluation and measurement were experimented, where the effectiveness thereof was verified. Analysis was also made on blood flow rate and perspiration volume related to skin temperature variation mechanism, which has provided a number of fundamental data. For the stress measuring technology, and stress relaxation by means of scent, researches were moved forward mainly on environment improvement and preliminary discussions. (NEDO)

  6. Fiscal 1998 research report. Survey on development and application of membranes with pores of micron to nano-meter sizes; 1998 nendo chosa kenkyu hokokusho. Makuro kara mikuro (nano mezo dai) size wo motsu, menburenmaku no kaihatsu narabi ni oyo ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Researches on preparation of membranes of various materials have been promoted by not systematic technique but separate techniques according to needs of concerned fields. To establish the efficient technique for membranes with pores of required uniform size according to needs of various industries, survey and study were made on process optimization and low-cost production method. Porous membrane is the leading candidate for new separation systems as separation medium in chemical industry, hot gas filtration for energy production and environmental purification engineering. The electrode, separator and gas storage medium of fuel cell vehicles and next-generation batteries require effective porous materials. The workshop on engineering porous materials held in May 1993 confirmed the time of following materials: High-efficiency gas separation membrane, chemical catalytic membrane, fuel cell electrode and absorbent for environmental purification. Development of inorganic membranes more excellent in high-temperature stability, strength, catalytic activity and corrosion resistance than previous polymer membranes is important. (NEDO)

  7. FY 2000 report on the results of the R and D of fundamental technologies of superconductivity applications. Development of technology to process low consumption power ultra high speed signals; 2000 nendo chodendo oyo kiban gijutsu kenkyu kaihatsu seika hokokusho. Teishohi denryoku chokosoku shingo shori gijutsu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-05-01

    In relation to the project on the R and D of fundamental technologies of superconductivity applications, the FY 2000 results of the design/fabrication of superconducting circuits were summarized. As to the development of technology to design superconducting circuits, an increase in circuit scale was tried targeting AD converter use modulator and decimation filter. As a result, operation was confirmed in element circuits of flux quantum multiplier circuit, feed back driver, DC isolator, etc. Concerning the development of technology for standard junction and integration, RHEED observations on the thin film surface before/after etching and YBa{sub 2}Cu{sub 3}O{sub 7-x} re-deposition were tried to be made, and the potentiality as monitoring technology was indicated. With respect to the fabrication of small scale circuits for demonstration, the design/trial fabrication were made of the basic pattern of SFQ circuit elements such as DC-SFQ, T-FF and SQUID for inductance rating. In regard to the development of technology to measure characteristics of superconducting circuits, a system was fabricated for processing and measuring output signals from {sigma}-{delta} modulators by semiconductor circuits, and it made the evaluation of AD converter performance possible. (NEDO)

  8. Achievement report for fiscal 1998 on the research and development of a basic technology to apply superconduction. Development of a low power consuming and high-speed signal processing technology; 1998 nendo chodendo oyo kiban gijutsu kenkyu kaihatsu seika hokokusho. Teishohi denryoku chokosoku shingo shori gijutsu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-05-01

    The development of a superconduction circuit designing and manufacturing technology has achieved the following results: with respect to designing a circuit for single flux quantum (SFQ), the 'high-speed high-accuracy AC converter for front end of software radio' was selected as the target for a prototype to demonstrate the performance of the SFQ circuit. In analyzing movements of different SFQ basic circuits, such circuit simulator software versions were introduced as Saber, WR-SPICE, Wins, and Microcap, different motion waveforms for basic theoretical circuits were derived to compare their performances, and the effectiveness was verified respectively. In trial fabrication of a small demonstration circuit, design parameters were acquired from SQUID. In developing the technology to measure superconduction circuit characteristics, the following results were obtained: with regard to the element measuring and evaluating technology, an in-plane distribution measuring device was started up; and with respect to the circuit characteristics measuring and evaluating technology, a measuring system with low noise and low magnetic field was introduced. Regarding the circuit high-speed characteristics evaluating technology, development was made on a micro-strip line and coplanar transmission route converter. (NEDO)

  9. FY 1999 report on the results of the introductory study on the global environmental industry technology. Investigational study on the environmental creation technology by the application of ecosystem information functions; 1999 nendo chikyu kankyo sangyo gijutsu ni kakawaru sendo kenkyu seika hokokusho. Seitaikei joho kino oyo ni yoru kankyo sozo gijutsu ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    To know the state of environmental pollution before it clearly exists, an investigational study was made on the technology to measure environmental stress at high sensitivity, in wide range and at high speed using functions to respond chemical substances, etc. which living organisms have. The FY 1999 results were outlined. As to the application of ecosystem information functions, survey was conduced paying attention to the environmental stress response of each of the bacteria, algae, plants and animals, responses viewed from the manifestation of immunity antibodies which occurs inside living organisms and changes at genetic level, and responses of algae and plants in case of specifying environmental pollutants. In relation to the application of the biological amplification mechanism, survey was carried out on environmental stress signals sent from plants, selection of environmental stress index plants, detection of environmental pollutants using bacteria, high sensitivity detection using animal (nematode), etc. Concerning the standardization of the environmental measuring technology by the application of ecosystem information functions, survey was conducted on artificial molecular recognition sensor, antibody device sensor, biochips, system construction, etc. (NEDO)

  10. Industrial science and technology research and development business for fiscal 1998. Research and development achievement report on glycoconjugate production and utilization technologies (Development of technologies of glycoconjugate-aided CO{sub 2} fixation and utilization); 1998 nendo sangyo kagaku gijutsu kenkyu kaihatsu jigyo. Fukugo toshitsu seisan riyo gijutsu no kenkyu kaihatsu seika hokokusho (fukugo toshitsu oyo nisanka tanso koteika yuko riyo gijutsu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Enzymatic and chemical methods were combined and methods were developed for synthesizing mucin type sugar chains and glycopeptides. The influence of sugar chain positions and structures on glycoconjugate stereo structures and physiological activities was analyzed, and glycopeptide structural activities and glycoconjugate remodelling were studied. To create an industrial advantage in a glycoprotein production system by use of zooblasts, sugar chain structure control through sugar transfase occurrence control was studied. For the production of man-adaptive sugar chains using yeast, new yeast variations were subjected to molecular breeding, and tested for the resultant improvement on productivity. Sugar chain marking and refining techniques and various responding mechanisms on the solid surface were elucidated, which enabled structure analyses using sugar chain recognition molecules such as lectins and antibodies. A database on interactions between sugar chains and sugar chain recognition molecules was constructed using the said findings. An analyzing program was also formulated tentatively. This paper also covers general surveys and studies on technologies of producing and utilizing glycoconjugates. (NEDO)

  11. Beta 3-adrenoreceptor regulation of nitric oxide in the cardiovascular system.

    Science.gov (United States)

    Moens, An L; Yang, Ronghua; Watts, Vabren L; Barouch, Lili A

    2010-06-01

    The presence of a third beta-adrenergic receptor (beta 3-AR) in the cardiovascular system has challenged the classical paradigm of sympathetic regulation by beta1- and beta2-adrenergic receptors. While beta 3-AR's role in the cardiovascular system remains controversial, increasing evidence suggests that it serves as a "brake" in sympathetic overstimulation - it is activated at high catecholamine concentrations, producing a negative inotropic effect that antagonizes beta1- and beta2-AR activity. The anti-adrenergic effects induced by beta 3-AR were initially linked to nitric oxide (NO) release via endothelial NO synthase (eNOS), although more recently it has been shown under some conditions to increase NO production in the cardiovascular system via the other two NOS isoforms, namely inducible NOS (iNOS) and neuronal NOS (nNOS). We summarize recent findings regarding beta 3-AR effects on the cardiovascular system and explore its prospective as a therapeutic target, particularly focusing on its emerging role as an important mediator of NO signaling in the pathogenesis of cardiovascular disorders. (c) 2010 Elsevier Ltd. All rights reserved.

  12. Causes of poverty: Voices of the rural poor from Oyo State, Nigeria ...

    African Journals Online (AJOL)

    This paper argues that poverty is multidimensional in nature and that its causes are multifaceted and extremely complex. It contends that poor people's own views of their descent into poverty are fundamental to understanding the complexity of poverty and to devising appropriate policy interventions that may lead to ...

  13. Wetland Farmers and Conflict Indices in Oyo State Fadama II Project ...

    African Journals Online (AJOL)

    International Journal of Applied Agriculture and Apiculture Research. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 4, No 1&2 (2007) >. Log in or Register to get access to full text downloads.

  14. HOUSEHOLDS’ VULNERABILITY TO POVERTY IN IBADAN METROPOLIS, OYO STATE, NIGERIA

    OpenAIRE

    Adepoju, Abimbola O.; Okunmadewa, Folusho Y.

    2011-01-01

    This paper empirically assessed vulnerability to poverty at the household level using a two-period panel data set obtained from 150 households sampled from two local government areas within Ibadan Metropolis. Data were analysed using descriptive statistics, poverty indices and probit regression analysis. Analysis of the socio-economic characteristics and their relationship with vulnerability to poverty revealed that large-sized households headed by men who were old, widowed, self-employed, un...

  15. The Impact of Credit on Poultry Farm Productivity in Oyo State, Nigeria

    African Journals Online (AJOL)

    The study examined the impact of credit constraint on the productivity of poultry farmers. The sources of credit and the amount demanded by the farmers in the study area were assessed. A random sample of farmers was taken in Ibadan metropolis. Primary data was collected on their socio-economic characteristics, sources ...

  16. Effects of flood on farmers in peri-urban area of Ibadan, Oyo state ...

    African Journals Online (AJOL)

    Using a snow ball research method, 60 farmers were selected from the six local governments in the peri – urban areas of Ibadan and data was collected using structured questionnaire on their personal characteristics, agricultural enterprises they engaged in, extent of farm damage, their knowledge of weather forecasts and ...

  17. The determinants of the demand and supply of eggs in Ibadan, Oyo ...

    African Journals Online (AJOL)

    This study assessed the major determinants for demand and supply of poultry products in Nigeria, using eggs as an example of poultry products and Ibadan as the case study area. Cross-Sectional survey was conducted for both demand and supply of eggs in Ibadan using pre-tested questionnaires. Stratified random ...

  18. Use of ICTs among fish farmers in Oyo State | Akinbile | Journal of ...

    African Journals Online (AJOL)

    The study concluded that fish farmers' behaviour to information is not poor but they do not have enough knowledge of ICTs, especially the modern ones which will make them exploit the potentials embedded in the use. Therefore there should be enough sensitization of fish farmers on the importance and advantages of the ...

  19. Technology and application of artificial actuality. Jinko genjitsukan sono gijutsu to oyo

    Energy Technology Data Exchange (ETDEWEB)

    Hirose, M. (The University of Tokyo, Tokyo (Japan). Faculty of Engineering)

    1991-11-20

    Concerning the technology for artificial actuality that has been noticed as the ultimate human interface, this report mainly describes its possibility as a new technique for operation and monitoring of its plant. The technology for artifical actuality is to use synthetic information by conputer in place of external recognition by the five senses to be put into the sense organs for the purpose of having pseudo-experience of the imaginary world. This technology has both aspects of a visualization technique and a communication technique. Considering the applicability of the technology for artificial actuality as a communication technique to a control room of a plant, it is expected that this technology would play an improtant role in transmission of the actual feeling. From the aspect of a visualization technique, it is possible to synthesize realistic images which are easy for prople to understand by using symbol information from a sensor, if the technology for artificeal actuality is applied. 7 figs.

  20. Microalgal lipid and its application; Bisai sorui shishitsu to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Matsunaga, T.; Nakamura, N. [Tokyo University of Agriculture and Technology, Tokyo (Japan)

    1995-10-20

    Docosahexaenoic acid (DHA) and icosapentaenoic acid (EPA) are valuable nutrients because they have important roles as the constituents of structural lipids in the nervous tissues of human brain and retina. In addition, DHA, EPA and other omega-3 fatty acids are important factors in the prevention of several human diseases. Fishes have been the dietary source of these fatty acids and they originate from the marine microbes upon which the fishes feed. Therefore, these marine microbes cultured under control could be utilized as alternative source of the omega-3 fatty acids. Marine microalgae are rich in DHA and EPA. However, very few species of freshwater algae contain significant amounts of these compounds. The marine unicellular alga Isochrysis galbana is well known as a source of such fatty acids and this species has been used extensively as a food source for aquaculture. The advantage of the marine microalgae such as I. galbana over other microbes as sources of polyunsaturated fatty acid is that this alga may be grown photosynthetically using carbon dioxide as the sole carbon source and seawater as a source of minerals. In this paper, we focused on the production of marine microalgae as an alternative source of polyunsaturated fatty acid. We have screened a number of species of marine microalga specifically for their ability to produce DHA and EPA and have optimized the growth conditions for I. galbana. The utilization of microalgae as feed for cultivation of conifers is also reported. Moreover, the applications of genetic engineering for EPA production are also demonstrated. 26 refs., 3 figs., 2 tabs.

  1. Willingness of tobacco retailers in Oyo State to participate in tobacco control programmes

    Directory of Open Access Journals (Sweden)

    Obioma Uchendu

    2018-01-01

    The sale of tobacco products is a profitable venture for retailers. Willingness to participate in tobacco control was high among the retailers. There is a need to raise awareness and co-opt tobacco retailers as change agents in tobacco control efforts.

  2. A Study among Bereaved Persons in Oyo and Oke-Ogun Areas

    African Journals Online (AJOL)

    User

    Mojoyinola, J. K. - Department of Social Work, Faculty of Education,. University of ... survey research design of the expost-facto type. The instruments ... The study established that social support had a significant impact on symptoms of emotional feelings associated with bereavement (F = 9.82, df = 1/198, P < .05), and also ...

  3. The State of Reading in Selected Secondary Schools in Oyo State ...

    African Journals Online (AJOL)

    Nekky Umera

    lack of reading materials, inadequate attention to reading, absence of reading on the time-table of schools and the influence of home videos, computer games and peer pressure (Kolawole, 1999 and Ogwuegbu, 2000). Statement of the Problem. It has been noted that the problems that affect reading at the secondary school.

  4. Economics of duck marketing in Oyo State | Saka | African Journal of ...

    African Journals Online (AJOL)

    Prices of drake (N557.30) were significantly higher than prices of duck (N424.90) across the locations and also ducks were sold at significantly higher prices during festival periods than non-festival periods (p<0.01). Marketing profit was 31.2% of the total marketing cost and 68.9% of the marketing margin, thus indicating that ...

  5. Determinants of Farmers' Access to Micro-Credit in Oyo State, Nigeria

    African Journals Online (AJOL)

    Apart from descriptive statistics and the Z-score test of significance, Tobit regression model was used to capture the expected dichotomous responses of respondents, and the determinants of their access to the credit in the study area. The study revealed that, most of the respondents (82.4%) were found between the active ...

  6. Small scale refrigeration(3). General applications; Kogata reito (3) oyo ippan

    Energy Technology Data Exchange (ETDEWEB)

    Shiraishi, Masao [Mechamical Engieneering Laboratory, Tsukuba (Japan)

    1999-09-25

    Presentations relating to general applications of small refrigerators were outlined. There were very few presentations of newly attempted or of concrete applications, in which some were paid attention on findings from research and development of small refrigerators. Performance of refrigerator being largely improved by removing contaminants, two studies on contamination in refrigerator had attracted notice. To expand application field, it is indispensable to improve cost vs. cooling performance, four studies on which were presented. (NEDO)

  7. Factors Affecting Effective Teaching and Learning of Economics in Some Ogbomosho High Schools, Oyo State, Nigeria

    Science.gov (United States)

    Ojo, Gbemisola Motunrayo; Nkoyane, Vusy

    2016-01-01

    This study was carried out to examine the present curriculum of Economics as a subject in some Ogbomoso Senior High Schools and to determine factors affecting effective teaching of economics in the schools. Variables such as number of students, teachers' ratio available textbooks were also examined. The study adopted descriptive design since it is…

  8. Evaluation of Aeromagnetic Data of Ilesha Area of Oyo State Nigeria ...

    African Journals Online (AJOL)

    ADOWIE PERE

    paper presented of the international association of seismologist and physics of the earth interior (IASPEI). 2009 conference at Cape Town, South Africa .Jan. 10th-16th. Kayode, J.S., Nyabaze, P., Adelusi, A.O. (2010). Ground magnetic study of Ilesha east, southwestern Nigeria,. African Journal of environmental science and.

  9. oral hygiene status of adolescents in a local government area of oyo ...

    African Journals Online (AJOL)

    User

    relationships (Rao et al. 2005). Poor oral hy- giene status often results in the accumulation of ... Periodontal disease and dental caries constitute major oral health problems in Nigeria with the prevalence being ... study on periodontal status and treatment need among Nigerian children, prevalence of dental caries was as ...

  10. Cultural perceptions of diarrhea and illness management choices among yoruba mothers in oyo state, Nigeria.

    Science.gov (United States)

    Okunribido, O O; Brieger, W R; Omotade, O O; Adeyemo, A A

    1997-01-01

    Home management of childhood illness is a norm throughout most of the world. Decisions about treatment are influenced by cultural perceptions of the illness, and diarrheal illnesses are no exception. A group of 473 mothers and their pre-school age children in rural communities outside Ibadan metropolis in Nigeria were followed over a two-month period. Data were collected on actual diarrhea illness episodes: mothers' names for these illnesses were recorded, and reported treatment actions were noted. Six major ethno-medical diarrheal illnesses were identified and were grouped broadly into watery diarrheas and dysentery-like diarrheas. Although few (40%) women used home-made sugar-salt solution (SSS) in case management, those who labeled their child's illness as a watery diarrhea were more likely to use SSS. Modern and herbal medicines were commonly and equally applied to both groups of diarrheal illnesses. While very few mothers reported decreased fluid intake by their children, many said the child had reduced appetite, especially if the child had a watery diarrhea. The findings indicate that twelve years after the national Oral Rehydration Therapy (ORT) Program was launched, few mothers practice the recommended actions of giving SSS, increasing food intake and avoiding drugs. Lack of attention to studies that describe the cultural basis for mothers' decisions could be part of the reason why the ORT has not been more successful.

  11. Recent application of shape memory alloy. Keijo kioku gokin no saikin no oyo kaihatsu kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Yamauchi, K. (Tokin Corp., Sendai (Japan). Materials Research Lab.)

    1993-07-20

    The Ti-Ni alloy, a typical shape memory alloy exhibits the CsCl type crystal structure in the matrix at an elevated temperature, and also exhibits a single crystal structure in the martensite phase at a lower temperature. The matrix phase at a high temperature is likely to be deformed. There is a brother phase which is different in orientation from the matrix phase in the low temperature phase. And the deformation takes place by the movement of their interface. No permanent deformation due to the dislocation takes place. A material which is deformed at a low temperature recovers its shape when it is heated because the material is not permanently deformed. This phenomenon is called the shape memory effect. Ten years have passed since the application research of the shape memory alloy was started. The progress gets slow-down. It becomes seldom that the topics concerning the newly developed products are published on magazines and newspapers. Many of the product developed in the past have been replaced by the other functional goods which were more reliable and less expensive than the shape memory alloy. This paper describes the recent situation of development. 15 refs., 8 figs., 1 tab.

  12. Space for Convenience Planning and Academic Performance of Secondary School Students in Oyo State, Nigeria

    Science.gov (United States)

    Yakubu, Suleman

    2017-01-01

    Every secondary school leaver is expected to be able to seek and gain admission into institutions of higher learning, both locally and internationally. However, this has become unattainable as a result of the poor academic performance seen in senior secondary school examinations; the quintessential example being the West African Senior School…

  13. A survey of library equipments in academic libraries in Ibadan, Oyo ...

    African Journals Online (AJOL)

    This study investigated the availability of library and equipment tools used in selected academic libraries in Ibadan, Nigeria. A structured questionnaire was developed to elicit information from the personnel of the selected libraries on the equipments and tools available in their respective libraries. The data were collated, ...

  14. Survey of meat preference within the working class of Oyo state ...

    African Journals Online (AJOL)

    24.66%), chevon (15.33%), chicken (13.33%), pork (10%) and mutton (5.33%) in reducing order of importance. Also, in terms of meat texture, 55.33% and 35.33% of the respondents preferred medium and soft meat respectively. The respondents ...

  15. HIV/AIDS in Oyo State, Nigeria: Analysis of Spatial Pattern of ...

    African Journals Online (AJOL)

    DrNneka

    Abstract. Understanding the geographical distribution of a disease is critical to policy makers' decision in deploying government resources to confronting such disease either with a view to totally eradicating it or at least control its spread. Since HIV/AIDS was first recognized in 1981 in New York, a number of factors, habits ...

  16. The State of Reading in Selected Secondary Schools in Oyo State ...

    African Journals Online (AJOL)

    Nekky Umera

    person who is unable to read and write is regarded as an illiterate, no matter the amount of commendable knowledge such a person possesses. The attempt to avoid the problem of illiteracy in the world has led to the considerable efforts being .... functional libraries, books and teachers who can promote reading at that level.

  17. HIV/AIDS in Oyo State, Nigeria: analysis of spatial pattern of ...

    African Journals Online (AJOL)

    This is in addition to Promiscuity and multiple sexual partner practices which spread the pandemic. The study however defined policy direction for government by churning out a number of recommendations which can help control the spread of the pandemic and care for the victims in the study area. Keywords: HIV/AIDS ...

  18. An evaluation of Oyo state library board in meeting the information ...

    African Journals Online (AJOL)

    The study is aimed at promoting the utilization and effectiveness of public library in Ibadan by secondary school students to enhance their academic perfor mance and exposure to international events. The study adopted descriptive survey research design. Questi onnaire was the main instrument for data collection, other ...

  19. Migration status and investment in rural productive activities: evidence from Oyo State, Nigeria.

    Science.gov (United States)

    Bankole, A

    1988-01-01

    "This paper presents an attempt to investigate the differential contributions of return migrants and non migrants [in Nigeria] to rural economy with respect to their level of investment in rural productive activities. The study is [a] sequel to the controversy generated by the selectivity theory which tends to suggest that return migrants are superior to, and more enterprising than non migrants. The relevant data were collected from 363 returnees and non migrants.... An examination of the differential characteristics of the two groups shows that returnees are slightly superior to their non migrant counterparts. The differential characteristics seem to translate to differences in investment level and the general conclusion is that return migrants are likely to invest more in rural productive activities than non migrants." (SUMMARY IN FRE AND ITA) excerpt

  20. Advance in lipid analysis and applications; Shibo bunseki no shinpo to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Takagi, T. [Nagoya Bunri College, Aichi (Japan)

    1998-10-20

    Recent development in lipid analysis for determination of compositions and characterization of molecular structures are reviewed. Important references on fundamental procedures including solid-phase extraction and size-exclusion chromatography are presented. Information on the development of chiral-phase chromatography of lipid enantiomers, discovery and utilization of delta-5 acids, use of stereospecific analysis of triacylglycerols by chiral-phase HPLC in biotechnology to obtain high-erucic oils, and methods for determination of optical purity of insect pheromones having aliphatic chain are discussed. 43 refs., 1 tab.

  1. HIV/AIDS in Oyo State, Nigeria: Analysis of Spatial Pattern of ...

    African Journals Online (AJOL)

    DrNneka

    0.01), there the test is significant. Hence, reject the Ho (null hypothesis) and then accept the alternative hypothesis i.e. HIV/AIDS prevalence is a function of promiscuity factor. Zone Prevalence - Multiple Sexual Partners. The cross tab below shows the result of the cross tabulation between the HIV/AIDS prevalence in the ...

  2. Stable gastric pentadecapeptide BPC 157-NO-system relation.

    Science.gov (United States)

    Sikiric, Predrag; Seiwerth, Sven; Rucman, Rudolf; Turkovic, Branko; Rokotov, Dinko Stancic; Brcic, Luka; Sever, Marko; Klicek, Robert; Radic, Bozo; Drmic, Domagoj; Ilic, Spomenko; Kolenc, Danijela; Aralica, Gorana; Stupnisek, Mirjana; Suran, Jelena; Barisic, Ivan; Dzidic, Senka; Vrcic, Hrvoje; Sebecic, Bozidar

    2014-01-01

    We reviewed stable gastric pentadecapeptide BPC 157-NO-system-relation, its close participation in Moncada's (maintained vascular integrity, platelets control) homeostatic healing response of NO-system to injury. Namely, BPC 157's particular healing effect also affects all events after vascular integrity loss (dependent on circumstances, it reduces either thrombosis (abdominal aorta anastomosis) or bleeding/thrombocytopenia (amputation, heparin, warfarin, aspirin)) and in a series of different injurious models, acute and chronic, BPC 157 consistently advances healing after severe injuries in various tissues spontaneously unable to heal; stimulates egr-1 and naB2 genes; exhibits high safety (LD1 not achieved)). Hypothesis, that BPC 157 (since formed constitutively in the gastric mucosa, stable in human gastric juice, along with significance of NO-synthase and the basal formation of NO in stomach mucosa, greater than that seen in other tissues) exhibits a general, effective competing both with L-arginine analogues (i. e., L-NAME) and L-arginine, and that this has some physiologic importance (NO-generation), later, practically supports its beneficial effects illustrating BPC 157 and NOsystem mutual (with L-NAME/L-arginine; alone and together) relations in (i) gastric mucosa and mucosal protection, following alcohol lesions, in cytoprotection course, NO-generation, and blood pressure regulation; (ii) alcohol acute/chronic intoxication, and withdrawal; (iii) cardiovascular disturbances, chronic heart failure, pulmonary hypertension, and arrhythmias; (iv) disturbances after hypokalemia and hyperkalemia, and potassium-cell membrane dysfunction; and finally, in (v) complex healing failure, proved by the fistulas healing, colocutaneous and esophagocutaneous. However, how this advantage of modulating NO-system (i. e., particular effect on eNOS gene), may be practically translated into an enhanced clinical performance remains to be determined.

  3. Fiscal 1997 R and D project on industrial science and technology under a consignment from NEDO. R and D of the application technology of functional protein complex (R and D of the high-functional maintenance technology for power plants); 1997 nendo sangyo kagaku gijutsu kenkyu kaihatsu jigyo Shin energy Sangyo gijutsu Sogo Kaihatsu Kiko itaku. Kinosei tanpakushitsu shugotai oyo gijutsu no kenkyu kaihatsu (hatsuden shisetsuyo kokino maintenance gijutsu kaihatsu) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    In order to develop advanced maintenance technology for power generation facilities, application technology of functional protein complexes is under investigation. On a light controllable enzymatic reaction system, construction of a light controllable bio-reactor by physico-chemical and bio- chemical techniques, and structure and functions of a photosynthetic protein complex using thermophilic cyanobacteria as material are under investigation. On a molecular mechanism for recognition and reaction of receptor proteins, structure analysis and engineering application of glutamate receptor channels of a synaptic transmission system, and basic study and material proteins for artificial structures using endocytic micromachinery, and a multi-enzyme system for synthesis of glycosphingolipids are under investigation. On a complex oxidation/reduction system by genetic engineering technique, construction of a high- efficiency stable system with functionally arranged oxidoreductase and electron transmission systems in biomembranes is in promotion. Research work was also made on molecular assemblies for a functional protein system. 166 refs., 91 figs., 9 tabs.

  4. Study of special spacer with damping device for suppressing wake galloping in tandem cables of cable-stayed bridges and its application to full scale bridge; Nendanseitai wo riyoshita heiretsu cable yo gensui kinotsuki spacer to sono jikkyo cable eno tekiyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Yoneda, M.; Setouchi, H.; Yoshioka, A. [Kawada Industries Inc., Tokyo (Japan); Shimoda, I.; Kawahara, S. [Oiles Corp., Tokyo (Japan)

    1996-12-20

    Cable vibration called wake galloping occurs in a cable-stayed bridge, in which cables are arranged tandem, and suppression of the vibration is an extremely important discussion item. This paper describes a complex eigen value analysis performed on tandem cables having lengths of 200 m and 100 m. The analysis revealed that damping characteristics added by a spacer system with a damping device (a type to install a visco-elastic body at about middle of the tandem cables via a jig) depend largely on torsional rigidity of the cables; and the characteristics may vary with difference in intervals between cable centers and that in cable diameters. A visco-elastic damping material of an asphalt system and a spacer with a damping device used as a spring material were fabricated and applied to a full scale bridge to identify quantitatively the damping addition characteristics of this system. Furthermore, the results of tests on the full scale bridge were compared with the result of the complex eigen value analysis, from which findings useful for actual works were obtained. 18 refs., 17 figs., 6 tabs.

  5. Introduction of fundamental approach for groundwater modeling 2. Groundwater recharge modeling. 2. 1 Rainfall infiltration and groundwater recharge. Chikasui modering no tameno deta kaiseki shuho no kiso 2. Chikasui eno kanyoryo no moderingu. 2. 1 Amamizu shinto[center dot]chikasui kanyo

    Energy Technology Data Exchange (ETDEWEB)

    Hirayama, Toshiaki

    1999-08-31

    How to handle the action of the material which makes subterranean water a main movement medium, the subterranean water during the underground environment of the obi water layer and the difficulty water layer and so on, and the amount of physics from the viewpoint of imitation is named as to and subterranean water simulation or modeling is sadist makes contact, and subterranean water sometimes deals with the surface of the earth basin and a soil basin though it is the element of the circulation like water literature. When a modeling technique is applied to the specific system, the work of the resolution of the element that that system is constructed and restructuring is necessary. The system which modeled the a series of work to resolve a component and which was restructured and which was restructured is said as the simulation model and the subterranean water model. The numerical value model whose generality is high and which is easy to deal with is often used, and the subterranean water model of the narrow sense points at the numerical value model. Though the various data of the geology, the nature of the soil, the water reason and others are necessary for the numerical value model, the entrance of the subterranean water, that is, the amount is one of the elements concerned with the analytic precision of the subterranean water model greatly. As for rain, river water is the supply process that the surface of the earth water such as water is added to the subterranean water as to the absorption. (NEDO)

  6. Introduction of fundamental approach for groundwater modeling 2. Groundwater recharge modeling. 2.1 Rainfall infiltration and groundwater recharge; Chikasui modering no tameno deta kaiseki shuho no kiso 2. Chikasui eno kanyoryo no moderingu. 2.1 Amamizu shinto{center_dot}chikasui kanyo

    Energy Technology Data Exchange (ETDEWEB)

    Hirayama, Toshiaki

    1999-08-31

    How to handle the action of the material which makes subterranean water a main movement medium, the subterranean water during the underground environment of the obi water layer and the difficulty water layer and so on, and the amount of physics from the viewpoint of imitation is named as to and subterranean water simulation or modeling is sadist makes contact, and subterranean water sometimes deals with the surface of the earth basin and a soil basin though it is the element of the circulation like water literature. When a modeling technique is applied to the specific system, the work of the resolution of the element that that system is constructed and restructuring is necessary. The system which modeled the a series of work to resolve a component and which was restructured and which was restructured is said as the simulation model and the subterranean water model. The numerical value model whose generality is high and which is easy to deal with is often used, and the subterranean water model of the narrow sense points at the numerical value model. Though the various data of the geology, the nature of the soil, the water reason and others are necessary for the numerical value model, the entrance of the subterranean water, that is, the amount is one of the elements concerned with the analytic precision of the subterranean water model greatly. As for rain, river water is the supply process that the surface of the earth water such as water is added to the subterranean water as to the absorption. (NEDO)

  7. NO Signaling in the Cardiovascular System and Exercise.

    Science.gov (United States)

    Fernandes, Tiago; Gomes-Gatto, Camila V; Pereira, Noemy P; Alayafi, Yahya R; das Neves, Vander J; Oliveira, Edilamar M

    2017-01-01

    Nitric oxide (NO) is a small molecule implicated in multiple signal transduction pathways thus contributing to the regulation of many cellular functions. The identification of NO synthase (NOS) isoforms and the subsequent characterization of the mechanisms of cell activation of the enzymes permitted the partial understanding of both the physiological and pathological processes. NO bioavailability plays an important role in the pathophysiology of cardiovascular disease and its reduction in endothelial cells is strictly associated to endothelial dysfunction which, in turn, correlates with cardiovascular mortality. Indeed, endothelial NO synthase (eNOS) has a key role in limiting cardiac dysfunction and remodeling in heart diseases, in part by decreasing myocyte hypertrophy. Conversely, exercise training is recommended to prevent and treat cardiovascular diseases-associated disorders at least by enhanced NO synthase activity and expression, and increased production of antioxidants, which prevents premature breakdown of NO. Exercise training may cause an improvement in endothelial function for both experimental animals and humans; Studies in both healthy subjects and patients with impaired NO-related vasorelaxation remarked exercise training ability to improve vascular structure and function and endothelial homeostasis. This chapter will briefly consider the importance of NO signaling in the maintenance of cardiovascular physiology, and discuss recent insights into the effect of exercise training on the signaling pathways that modulate NO synthesis and degradation in health and cardiovascular disease. In addition, we will highlight the molecular mechanisms via which microRNAs (miRs) target NO signaling in the cardiovascular system, and NO as a candidate molecule for development of new therapies.

  8. Angiotensin II type 1 receptor blockade partially attenuates hypoxia-induced pulmonary hypertension in newborn piglets: relationship with the nitrergic system

    Energy Technology Data Exchange (ETDEWEB)

    Camelo, J.S. Jr. [Departamento de Puericultura e Pediatria, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Martins, A.R. [Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Instituto de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, MG (Brazil); Rosa, E. [Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Ramos, S.G. [Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SPBrasil (Brazil); Hehre, D.; Bancalari, E.; Suguihara, C. [Department of Pediatrics, Division of Neonatology, Neonatal Developmental Biology Laboratory, University of Miami Miller School of Medicine, Miami, FL (United States)

    2012-02-10

    The objective of this study was to observe possible interactions between the renin-angiotensin and nitrergic systems in chronic hypoxia-induced pulmonary hypertension in newborn piglets. Thirteen chronically instrumented newborn piglets (6.3 ± 0.9 days; 2369 ± 491 g) were randomly assigned to receive saline (placebo, P) or the AT{sub 1} receptor (AT{sub 1}-R) blocker L-158,809 (L) during 6 days of hypoxia (FiO{sub 2} = 0.12). During hypoxia, pulmonary arterial pressure (Ppa; P < 0.0001), pulmonary vascular resistance (PVR; P < 0.02) and the pulmonary to systemic vascular resistance ratio (PVR/SVR; P < 0.05) were significantly attenuated in the L (N = 7) group compared to the P group (N = 6). Western blot analysis of lung proteins showed a significant decrease of endothelial NOS (eNOS) in both P and L animals, and of AT{sub 1}-R in P animals during hypoxia compared to normoxic animals (C group, N = 5; P < 0.01 for all groups). AT{sub 1}-R tended to decrease in L animals. Inducible NOS (iNOS) did not differ among P, L, and C animals and iNOS immunohistochemical staining in macrophages was significantly more intense in L than in P animals (P < 0.01). The vascular endothelium showed moderate or strong eNOS and AT{sub 1}-R staining. Macrophages and pneumocytes showed moderate or strong iNOS and AT{sub 1}-R staining, but C animals showed weak iNOS and AT{sub 1}-R staining. Macrophages of L and P animals showed moderate and weak AT{sub 2}-R staining, respectively, but the endothelium of all groups only showed weak staining. In conclusion, pulmonary hypertension induced by chronic hypoxia in newborn piglets is partially attenuated by AT{sub 1}-R blockade. We suggest that AT{sub 1}-R blockade might act through AT{sub 2}-R and/or Mas receptors and the nitrergic system in the lungs of hypoxemic newborn piglets.

  9. Fiscal 1997 report on the results of the international standardization R and D. Study of safety of laser and light emitting diodes (LED) for human body; 1997 nendo seika hokokusho kokusai hyojun soseigata kenkyu kaihatsu. Laser, oyobi hakko diode (LED) ni taisuru jintai eno anzensei no kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    LD and LED are used in a wide range from telecommunication systems to residential/commercial equipment. Optical semiconductor devices existing in the domain between LD and LED are also being commercialized. In the field of this project, there are a lot of contradictions about the international standards. For the purpose of promoting the common understanding of standards and improving the consistency, the paper examined mainly safety, principles of motion and various applications of laser and LED, and developed international standards for the output measuring method. At the same time, the paper systematically studied the biological safety, determined regulatory values which were backed up as safety standards, and proposed/worked out a draft for new international standardization for safety of LED. Further, by the definition of laser in general, standards related to a lot of TCs among ISO and IEC standards were harmonized for the common understanding on the basis of a common idea. 40 refs., 163 figs., 6 tabs.

  10. Design and construction of reclamation for site of Tachibana Bay Power Plant. Large-scale reclamation of soft sedimentary clay ground; Tachibanawan hatsudensho shikichi zosei koji ni okeru umetate no sekkei to seko. Nanjaku na taiseki nenseido jibanjo eno daikibo umetate

    Energy Technology Data Exchange (ETDEWEB)

    Suesawa, H.; Iwahara, H.; Nakahiro, M. [Shikoku Electric Power Co., Inc., Kagawa (Japan)

    1997-05-05

    This paper describes site establishment and reclamation construction at a power plant (2.8 million kW, single coal combustion) being built at Kokatsu Island (Tachibana Bay) in Tokushima Prefecture. The western side of Kokatsu Island was cut off, and the cut-out soil and rocks were used to establish a site of 25 ha including a reclaimed land. Presence of soft clayish soil deposited to a thickness of about 10 m on the sea bottom of the reclaimed area required suppression of slide and large deformation of the clayish soil bed. Therefore, the reclamation was carried out in three steps. The first reclamation was used as a preceding load to accelerate compaction during the standing period before the second reclamation and as a pressing banking during the second fill-up. Thin-layer spreading construction (simplified floating conveyer system or simplified FCS) was carried out from above the sea. The second reclamation was carried out by one-side pressing from the land side upon completion of the first reclamation and after the standing, and was used as a pressing banking for the third fillingup. The third reclamation was executed by using one-side pressing on the land, completing the reclamation and finishing up to the predetermined ground height. The step-wise reclamation can suppress large deformation and slide of the clayish soil bed at the sea bottom. The simplified FCS spreading construction is an effective method when the reclaimed layer thickness should be controlled minutely. 6 refs., 16 figs., 7 tabs.

  11. Role of the eNOS-NO System in Regulating the Antiproteinuric Effects of VEGF Receptor 2 Inhibition in Diabetes

    Directory of Open Access Journals (Sweden)

    Andrew Advani

    2013-01-01

    Full Text Available Subtle perturbations in intraglomerular VEGF/VEGFR-2 signaling or in the influencing microenvironment can profoundly affect renal function, resulting in the apparently paradoxical observation that VEGF blockade attenuates proteinuria development in experimental diabetes despite exerting the opposite effect under other circumstances. In the present study, we sought to explore the role of eNOS-NO activity in regulating the differential response to VEGF blockade in the diabetic and nondiabetic settings. In a rodent model of accelerated renal injury, the transgenic (mRen-227 (Ren-2 rat, VEGFR-2 inhibition with the small molecule vandetanib resulted in an increase in urine protein excretion preceding a subsequent rise in systolic blood pressure. When compared to their normoglycaemic counterparts, diabetic Ren-2 rats exhibited an increase in the renal expression of eNOS and in urinary excretion of nitric oxide (NO metabolites. In contrast to the heavy proteinuria observed with vandetanib in nondiabetic TGR(mRen-227 rats, VEGFR-2 inhibition reduced urine protein excretion in diabetic animals, despite a comparable magnitude of histological injury. However, proteinuria was markedly increased by concomitant treatment of diabetic Ren-2 rats with vandetanib and the nitric oxide synthase inhibitor L-NAME. These observations highlight the pivotal role that the eNOS-NO system plays in regulating the biologic response to VEGF within the glomerulus.

  12. Role of the eNOS-NO system in regulating the antiproteinuric effects of VEGF receptor 2 inhibition in diabetes.

    Science.gov (United States)

    Advani, Andrew; Connelly, Kim A; Advani, Suzanne L; Thai, Kerri; Zhang, Yuan; Kelly, Darren J; Gilbert, Richard E

    2013-01-01

    Subtle perturbations in intraglomerular VEGF/VEGFR-2 signaling or in the influencing microenvironment can profoundly affect renal function, resulting in the apparently paradoxical observation that VEGF blockade attenuates proteinuria development in experimental diabetes despite exerting the opposite effect under other circumstances. In the present study, we sought to explore the role of eNOS-NO activity in regulating the differential response to VEGF blockade in the diabetic and nondiabetic settings. In a rodent model of accelerated renal injury, the transgenic (mRen-2)27 (Ren-2) rat, VEGFR-2 inhibition with the small molecule vandetanib resulted in an increase in urine protein excretion preceding a subsequent rise in systolic blood pressure. When compared to their normoglycaemic counterparts, diabetic Ren-2 rats exhibited an increase in the renal expression of eNOS and in urinary excretion of nitric oxide (NO) metabolites. In contrast to the heavy proteinuria observed with vandetanib in nondiabetic TGR(mRen-2)27 rats, VEGFR-2 inhibition reduced urine protein excretion in diabetic animals, despite a comparable magnitude of histological injury. However, proteinuria was markedly increased by concomitant treatment of diabetic Ren-2 rats with vandetanib and the nitric oxide synthase inhibitor L-NAME. These observations highlight the pivotal role that the eNOS-NO system plays in regulating the biologic response to VEGF within the glomerulus.

  13. Physiological mechanisms of vascular response induced by shear stress and effect of exercise in systemic and placental circulation.

    Directory of Open Access Journals (Sweden)

    Iván eRodríguez

    2014-09-01

    Full Text Available Physiological vascular function regulation is essential for cardiovascular health and depends on adequate control of molecular mechanisms triggered by endothelial cells in response to mechanical and chemical stimuli induced by blood flow. Endothelial dysfunction is one of the main risk factors of cardiovascular pathology, where the imbalance between the synthesis of vasodilator and vasoconstrictor molecules is common in the development of vascular disorders in systemic and placental circulation. In the placenta, an organ without autonomic innervations, the local control of vascular tone is critical for maintenance of fetal growth and mechanisms that underlie shear stress response induced by blood flow are essential during pregnancy. In this field, shear stress induced by moderate exercise is one of the most important mechanisms to improve vascular function through nitric oxide (NO synthesis and stimulation of mechanical response of endothelial cells triggered by ion channels, caveolae, endothelial NO synthase (eNOS and vascular endothelial growth factor (VEGF, among others. The demand for oxygen and nutrients by tissues and organs, especially in placentation and pregnancy, determines blood flow parameters and physiological adaptations of vascular beds for covering metabolic requirements. In this regard, moderate exercise versus sedentarism shows potential benefits for improving vascular function associated with the enhancement of molecular mechanisms induced by shear stress. In this review, we collect evidence about molecular bases of physiological response to shear stress in order to highlight the relevance of moderate exercise-training for vascular health in adult and fetal life.

  14. Promoting aquatic tourism potentials of Nigerian parks: a case study ...

    African Journals Online (AJOL)

    ... and aquatic recreational tourism within Nigerian park system. Sampling was done during the wet and dry seasons of the survey period in Ogun river of Old Oyo National park, using monofilament gillnets of different sizes ranging from 38mm to 127mm with graded hooks.Physico-chemical properties of the river was equally ...

  15. Career Commitment: Interplay of Core-Self-Evaluation and Reward ...

    African Journals Online (AJOL)

    This study investigated the joint influence of core self-evaluation (self efficacy, self-esteem, work locus of control and neuroticism) and reward system on carrer commitment of local government employees in Oyo State, Nigeria. The study adopted descriptive survey research design. A total of 2,040 respondents were selected ...

  16. Browse Title Index

    African Journals Online (AJOL)

    Items 401 - 414 of 414 ... Vol 14, No 1 (2017), Virtual law library as a veritable tool for justice delivery system in Nigeria: an investigative study of Oyo state high court, Abstract. Hawwau A. Moruf, Pius O. Olaojo. Vol 2, No 2 (2005), Virtual libraries in Nigeria: dream or reality, Abstract. Jide Owoeye. Vol 7, No 1 (2010), Web Of ...

  17. TABLE PREVALENCE OF GIT NEMATODES IN CATTLE

    African Journals Online (AJOL)

    User

    1Faculty of Veterinary Medicine, P.M.B.1515, University of Ilorin, Ilorin Kwara State, Nigeria. 2Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State., Nigeria. *Corresponding author: drsaameen@yahoo.com ... under the traditional husbandry system (Fakae,. 1990). Financial costs of internal parasitism ...

  18. An artificial compression method for ENO schemes - The slope modification method. [essentially nonoscillatory

    Science.gov (United States)

    Yang, Huanan

    1990-01-01

    A simple and effective method of artificial compression is introduced. This method is based on a modification of the slopes of the END (essentially nonoscillatory) reconstruction and, with the help of suitable chosen parameters, greatly improves the resolution of the contact discontinuities. Numerical examples are provided to test the performance of the method and to give some suggestions as to the choice of the parameters.

  19. effect of dl-carnitine on tissue content of ad eno ine triphospha te ...

    African Journals Online (AJOL)

    BSN

    myocardium is said to be ischaemic when its oxygen demand is not correspondingly met by coronary supply of oxygen (coronary blood supply). This often results in metabolic disorders in which essentially there is a change from aerobic to anaerobic metabolism leading the accumulation of lactic acid which together with ...

  20. Application of wavelet transform to seismic data; Wavelet henkan no jishin tansa eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Nakagami, K.; Murayama, R.; Matsuoka, T. [Japan National Oil Corp., Tokyo (Japan)

    1996-05-01

    Introduced herein is the use of the wavelet transform in the field of seismic exploration. Among applications so far made, there are signal filtering, break point detection, data compression, and the solution of finite differential equations in the wavelet domain. In the field of data compression in particular, some examples of practical application have been introduced already. In seismic exploration, it is expected that the wavelet transform will separate signals and noises in data in a way different from the Fourier transform. The continuous wavelet transform displays time change in frequency easy to read, but is not suitable for the analysis and processing large quantities of data. On the other hand, the discrete wavelet transform, being an orthogonal transform, can handle large quantities of data. As compared with the conventional Fourier transform that handles only the frequency domain, the wavelet transform handles the time domain as well as the frequency domain, and therefore is more convenient in handling unsteady signals. 9 ref., 8 figs.

  1. Truncus Arteriosus in a 43 year old Male: Case Report | Ng'eno ...

    African Journals Online (AJOL)

    Browse By Category · Browse Alphabetically · Browse By Country · List All Titles · Free To Read Titles This Journal is Open Access. Featuring journals from 32 Countries: Algeria (5); Benin (2); Botswana (3); Burkina Faso (3); Cameroon (8); Congo, Republic (1); Côte d'Ivoire (4); Egypt, Arab Rep. (14); Eritrea (1); Ethiopia (30) ...

  2. Spatial autocorrelation method using AR model; Kukan jiko sokanho eno AR model no tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, H.; Obuchi, T.; Saito, T. [Iwate University, Iwate (Japan). Faculty of Engineering

    1996-05-01

    Examination was made about the applicability of the AR model to the spatial autocorrelation (SAC) method, which analyzes the surface wave phase velocity in a microtremor, for the estimation of the underground structure. In this examination, microtremor data recorded in Morioka City, Iwate Prefecture, was used. In the SAC method, a spatial autocorrelation function with the frequency as a variable is determined from microtremor data observed by circular arrays. Then, the Bessel function is adapted to the spatial autocorrelation coefficient with the distance between seismographs as a variable for the determination of the phase velocity. The result of the AR model application in this study and the results of the conventional BPF and FFT method were compared. It was then found that the phase velocities obtained by the BPF and FFT methods were more dispersed than the same obtained by the AR model. The dispersion in the BPF method is attributed to the bandwidth used in the band-pass filter and, in the FFT method, to the impact of the bandwidth on the smoothing of the cross spectrum. 2 refs., 7 figs.

  3. PArthENoPE: Public Algorithm Evaluating the Nucleosynthesis of Primordial Elements

    Energy Technology Data Exchange (ETDEWEB)

    Pisanti, O.; Cirillo, A.; Esposito, S.; Iocco, F.; Mangano, G.; Miele, G.; Serpico, P.D.

    2007-05-04

    We describe a program for computing the abundances of light elements produced during Big Bang Nucleosynthesis which is publicly available at http://parthenope.na.infn.it/. Starting from nuclear statistical equilibrium conditions the program solves the set of coupled ordinary differential equations, follows the departure from chemical equilibrium of nuclear species, and determines their asymptotic abundances as function of several input cosmological parameters as the baryon density, the number of effective neutrino, the value of cosmological constant and the neutrino chemical potential.

  4. Riik hakkab Via Baltica maanteed neljarealiseks joonistama / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 25. sept. lk. 2. Pärnu maavalitsuse ja maanteeameti eestvedamisel alustatakse Tallinnast Iklani kulgeva Via Baltica liiklussoone teemaplaneeringut, mille järgi tuleb Pärnumaal leida lahendused Are ja Pärnu ümbersõidule ning Nurme õgvendusele. Arvamust avaldab Are vallavanem Jaanus Männik

  5. Influence of life line stoppage on building equipments; Lifeline tozetsu no kenchiku setsubi eno eikyo

    Energy Technology Data Exchange (ETDEWEB)

    Sadohara, S. [Yokohama National University, Yokohama (Japan). Faculty of Engineering

    1995-06-05

    Our daily life is being supported by electricity in various ways. This paper, explains the influence of the stoppage of the electricity, affecting infrastructures, buildings and people`s daily life based on the survey result of a long term electricity stoppage in Hiroshima City in September 1991. This paper also introduces the survey result of emergency generators operation in Japan and in the U.S. Last, the ways and ideas to make lifeline reliability higher in the future are summarized. 1 ref., 11 figs., 7 tabs.

  6. effect of dl-carnitine on tissue content of ad eno ine triphospha te

    African Journals Online (AJOL)

    BSN

    wavelength 340nm with a tungsten lamp light source. Into each cuvettes 1 ml of double distilled water was added, followed by 1 ml of. 0.2N\\ TRIS buffer .... prevent the depletion of myocardial tissue content of ATP and CP by experimental ischacmia. ACKNOWLEDGEMENT: Special thanks to staff of Postgraduate Studies.

  7. Fiscal 1997 R and D project on industrial science and technology under a consignment from NEDO. R and D of the synthesis and processing of advanced biomaterials (fixation and effective utilization of carbon dioxide using peptides); 1997 nendo Shin energy Sangyo Gijutsu Sogo Kaihatsu Kiko itaku. Senshin bio zairyo no sosei kako gijutsu no kenkyu kaihatsu (peptide oyo nisanka tanso koteika yuko riyo gijutsu) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    In fiscal 1997, study was made on formation of peptides with a biogenic function and molecular recognition function, and peptide materials. The existing automatic design technique of peptides including non-natural amino acids was applied to design of complementary peptides of proteins with known structures to optimize a development technique of ligand. Practical function of peptides as advanced receptor ligand was verified by applying the existing conformation control technique to stable holding of peptide active conformation. Creation of bio-materials, and development of preparation technology of bio-substrates and materializing technology of functional molecular materials were carried out. To construct an electron transfer system by self- organized body of peptides formed on a substrate, a peptide molecular device was prepared and verified using linkers. To use peptides as photoelectron functional molecule, metallized peptide with main helix structure was prepared. The possibility of molecular materials with a fixation function of CO2 was examined. A high-sensitivity bio-sensor was also mentioned. 75 refs., 130 figs., 25 tabs.

  8. The Design and Use of a Viscometer for the Study of Reacting Resin Systems.

    Science.gov (United States)

    1981-11-01

    bandwidth does exist and there can be derived empirically a sequence of actions necessary to produce the requisite composite quality taking into account...Othe Refee eNos N/A N/AStructures 15. Distribution statement (a) ContrIled by - (b) Specia limiaon (if aoy) - 16. Descriptors (Keywords) (Descriptors

  9. System Budgets

    DEFF Research Database (Denmark)

    Jeppesen, Palle

    1996-01-01

    The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers.......The lecture note is aimed at introducing system budgets for optical communication systems. It treats optical fiber communication systems (six generations), system design, bandwidth effects, other system impairments and optical amplifiers....

  10. Automated Construction Management System (ACMS). Volume 2. Program Documentation.

    Science.gov (United States)

    1984-06-01

    of program IF mcmd<>𔃼’ @ 21,0 @ 21, 0 SAY "Continue adding attachments or individule components? (yin)" GET mmore PICTURE !0 READ CLEAR GETS ENDIF...meno2=eno).AND.(.NOT.EOF) IF (!(m)=’A’).AND.(title=l) @ row,5 SAY "ATTACHMENTS:" STORE 0 TO title ELSE IF (!(m)=’I’).AND.(title=l) @ row,5 SAY " INDIVIDULE

  11. Sildenafil Protects against Myocardial Ischemia-Reperfusion Injury Following Cardiac Arrest in a Porcine Model: Possible Role of the Renin-Angiotensin System

    Science.gov (United States)

    Wang, Guoxing; Zhang, Qian; Yuan, Wei; Wu, Junyuan; Li, Chunsheng

    2015-01-01

    Sildenafil, a phosphodiesterase-5 inhibitor sold as Viagra, is a cardioprotector against myocardial ischemia/reperfusion (I/R) injury. Our study explored whether sildenafil protects against I/R-induced damage in a porcine cardiac arrest and resuscitation (CAR) model via modulating the renin-angiotensin system. Male pigs were randomly divided to three groups: Sham group, Saline group, and sildenafil (0.5 mg/kg) group. Thirty min after drug infusion, ventricular fibrillation (8 min) and cardiopulmonary resuscitation (up to 30 min) was conducted in these animals. We found that sildenafil ameliorated the reduced cardiac function and improved the 24-h survival rate in this model. Sildenafil partly attenuated the increases of plasma angiotensin II (Ang II) and Ang (1–7) levels after CAR. Sildenafil also decreased apoptosis and Ang II expression in myocardium. The increases of expression of angiotensin-converting-enzyme (ACE), ACE2, Ang II type 1 receptor (AT1R), and the Ang (1–7) receptor Mas in myocardial tissue were enhanced after CAR. Sildenafil suppressed AT1R up-regulation, but had no effect on ACE, ACE2, and Mas expression. Sildenafilfurther boosted the upregulation of endothelial nitric oxide synthase (eNOS), cyclic guanosine monophosphate (cGMP) and inducible nitric oxide synthase(iNOS). Collectively, our results suggest that cardioprotection of sildenafil in CAR model is accompanied by an inhibition of Ang II-AT1R axis activation. PMID:26569234

  12. Sildenafil Protects against Myocardial Ischemia-Reperfusion Injury Following Cardiac Arrest in a Porcine Model: Possible Role of the Renin-Angiotensin System

    Directory of Open Access Journals (Sweden)

    Guoxing Wang

    2015-11-01

    Full Text Available Sildenafil, a phosphodiesterase-5 inhibitor sold as Viagra, is a cardioprotector against myocardial ischemia/reperfusion (I/R injury. Our study explored whether sildenafil protects against I/R-induced damage in a porcine cardiac arrest and resuscitation (CAR model via modulating the renin-angiotensin system. Male pigs were randomly divided to three groups: Sham group, Saline group, and sildenafil (0.5 mg/kg group. Thirty min after drug infusion, ventricular fibrillation (8 min and cardiopulmonary resuscitation (up to 30 min was conducted in these animals. We found that sildenafil ameliorated the reduced cardiac function and improved the 24-h survival rate in this model. Sildenafil partly attenuated the increases of plasma angiotensin II (Ang II and Ang (1–7 levels after CAR. Sildenafil also decreased apoptosis and Ang II expression in myocardium. The increases of expression of angiotensin-converting-enzyme (ACE, ACE2, Ang II type 1 receptor (AT1R, and the Ang (1–7 receptor Mas in myocardial tissue were enhanced after CAR. Sildenafil suppressed AT1R up-regulation, but had no effect on ACE, ACE2, and Mas expression. Sildenafilfurther boosted the upregulation of endothelial nitric oxide synthase (eNOS, cyclic guanosine monophosphate (cGMP and inducible nitric oxide synthase(iNOS. Collectively, our results suggest that cardioprotection of sildenafil in CAR model is accompanied by an inhibition of Ang II-AT1R axis activation.

  13. FY 1998 report on the results of the research and development of fundamental technologies for superconductor applications; 1998 nendo chodendo oyo kiban gijutsu kenkyukaihatsu seika hokokusho. Chodendo oyo kiban gijutsu keknyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-05-01

    As to the research on the superconductor manifestation mechanism, Y123 monocrystal which was partially replaced with Zn and Ni was manufactured, and it was made clear that the addition of these impurities greatly change anisotropy of superconductors. It was made clear that instability of lattice and magnetism is included in high temperature superconductor. Also made clear was an important role in the Nb/Ba replacement in existence of magnetic flux melting phase transfer and the magnetic field induced pinning effect. The manufacture of Y123 bicrystal membrane was successful by the liquid phase epitaxy method. The manufacture of non-copper base K-Bi-O new superconductor was successful by the ultra-high pressure synthesis method. The stabilized process was established for large size crystals of 15-20mm square in Y base and of 20-25mm square in Nd base. Also successful was the growth by LPE method of crystal with thick membrane of 20mm x 20mm x 10{mu}m on the MgO monocrystal substrate. The non-bicrystalization of Y base Nd base monocrystal was successful, and the peak phenomenon control in Jc-H was found out. By neutron irradiation, the acquisition magnetic field of 3.7T at 77K was achieved using bulk Y base 123 material with diameter of 3cm. And, the critical current density of 60,000 A/cm{sup 2} was achieved at 77K and 3T. (NEDO)

  14. Immune System

    Science.gov (United States)

    ... Counselors Kidney Stones Brain and Nervous System Immune System KidsHealth > For Teens > Immune System Print A A ... put us out of commission. What the Immune System Does The immune (pronounced: ih-MYOON) system, which ...

  15. System Identification

    NARCIS (Netherlands)

    Keesman, K.J.

    2011-01-01

    Summary System Identification Introduction.- Part I: Data-based Identification.- System Response Methods.- Frequency Response Methods.- Correlation Methods.- Part II: Time-invariant Systems Identification.- Static Systems Identification.- Dynamic Systems Identification.- Part III: Time-varying

  16. Microbiological Safety Assessment of Fermented Cassava Flour “Lafun” Available in Ogun and Oyo States of Nigeria

    Directory of Open Access Journals (Sweden)

    A. O. Adebayo-Oyetoro

    2013-01-01

    Full Text Available The microorganisms involved in the fermentation and spoilage of fermented cassava flour were investigated. The water samples used at the different processing sites were also investigated to determine their safety status. There was predominance of Staphylococcus aureus, Aspergillus spp., and Escherichia coli in all samples. Coliforms were observed to be present in all of the processing water. In the fermented cassava flour, the total bacterial count ranged between 4.9×106 cfu/mL from Eleso, Bakatari, and Oja Odan processing sites and 8.10×106 cfu/mL in Eruku processing site. The majority of the microorganisms involved in the spoilage of “lafun” were found to be Aspergillus niger which ranged between 4.6×105 cfu/mL in Eleso and 8.1×105 cfu/mL in Kila. The control sample prepared in the laboratory had a low microbial load compared to samples collected from various sites and markets.

  17. Determinants of adoption of vitamin A bio-fortified cassava variety among farmers in Oyo State, Nigeria

    Directory of Open Access Journals (Sweden)

    O. O. Ologunde

    2017-01-01

    Full Text Available The success of any agricultural innovation depends on its adoption by farmers. The study evaluates the determinants of adoption of vitamin A bio-fortified cassava variety among farmers by investigating the level of adoption and determinants of adoption among farmers. Three stages of random sampling procedure were used to select 240 cassava farmers. The data obtained were analyzed with descriptive statistics and a logit regression model. The study revealed that the level of adoption of vitamin A bio-fortified cassava variety is low (38.72%. The study also revealed that access to media, contact with extension agents, access to vitamin A bio-fortified cassava stem, amongst others, are the determinants of adoption of vitamin A bio-fortified cassava variety in the study area. It is therefore recommended that awareness about the new cassava technology should be prioritized to sensitize the farmers, and stems of these cassava varieties should be readily made available to farmers to take advantage of the benefit of the innovation.

  18. Feature of high velocity oxygen-fuel flame spraying; Kosoku flame yoshaho no tokucho to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Shimizu, Y.; Sakaki, K. [Shinshu University, Nagano (Japan). Faculty of Engineering

    1996-05-01

    A description is given about the high velocity flame spraying method. In this method, fuel and oxygen under high pressure are supplied to a spraying gun, a supersonic stream of flame is jetted out of a fine nozzle, and spray particles are injected into the flame to impinge on the substrate surface at a very high speed for the formation of a coating. This method is advantageous in that the spray particles are higher in flying speed than in other spraying methods, that the produced coating is dense and close and excellent in adhesion, that the flame temperature is relatively low, and that the spray material is suppressed in terms of phase transformation, oxidation, and decomposition. This spraying technique is disadvantageous in that the spray materials that it can use are limited in variety because this method meets difficulties in spraying upon high melting-point metal or ceramics. This paper also outlines the spraying devices (chamber combustion type and throat combustion type) and the characteristics of produced coatings, and spray materials and their application (centering about carbide thermit spraying) are mentioned. 23 refs., 6 figs., 2 tabs.

  19. Effects of Self-Efficacy Training Programmes on Adolescents' Sexual Risk-Taking Behaviour in Oyo State, Nigeria

    Science.gov (United States)

    Musa, Titilayo Monsurat

    2017-01-01

    Adolescents' sexual risk-taking behavioural issues have generated concerns among parents, teachers and social workers. The study examined the effects of self-efficacy training programmes on adolescents' sexual risk-taking behaviour and also investigated whether socio-economic status and gender would moderate the effects of treatment on sexual…

  20. An observational study of road safety around selected primary schools in Ibadan municipality, Oyo State, Southwestern Nigeria.

    Science.gov (United States)

    Sangowawa, Adesola O; Adebiyi, Akindele O; Faseru, Babalola; Popoola, Olusola J

    2012-01-01

    Child pedestrians have been identified as vulnerable road users. Although walking as a means of transport has health and other benefits, it exposes children to the risk of road traffic injuries. This study was conducted to assess the availability of road safety features around government-owned primary schools in Ibadan municipality. A multistage sampling technique was used to select 46 of the 74 schools in the study area. Some (11) of the selected schools were sited within the same premises and shared a common entrance; thus a total of 35 school premises were eventually observed. Trained research assistants observed the school environment around the selected schools for road safety features such as location of schools, presence of "school", "child crossing" and "speed limit" road signs, and presence of traffic calming devices (road bumps or zebra crossing). Five (14%) of the schools were located on major roads and eight (23%) had road signs indicating that a school was nearby. Seven (20%) had road bumps close to the school, 15 (43%) had a warden who assisted children to cross, and none had a zebra crossing. Five (14%) schools had pedestrian sidewalks. The study revealed that the environment around a number of the observed schools in the municipality compromised the pupils' road safety. The local government, school authorities, parents, and road safety professionals need to institute definite measures to enhance the road safety environment around schools in the municipality.

  1. Prevalence of Toxoplasma gondii and HIV infection among pregnant women in Ibadan North Local Government, Oyo State.

    Science.gov (United States)

    Awobode, H O; Olubi, I C

    2014-12-01

    Toxoplasmosis, a disease caused by Toxoplasma gondii poses a risk of congenital infection during pregnancy in infected women. The disease poses a threat of ocular and neurological sequelae in congenitally ilfected infants and HIV patients; however, there is little valuable information on its prevalence in Ibadan. A cross sectional study of pregnant women attending antenatal care in three primary healthcare centres in Ibadan was carried out. 179 women were screened for T gondii infection using PCR, 83 of these were also screened for HIV. Forty nine (27.4%) were positive for T gondii, and 2(2.4%) were HIV positive (P = 0.002, OR = 0.28, CL = 95%). 27 (15.1%) of the infected women were in third trimester, with 20 (11.2%) and 2 (1.1%) in second and first trimester respectively. 18 (10.1%) positive cases were identified among the primigravidas and 31 (17.2%) among the multigravidas. Risk factors associated with Toxoplasma infection were assessed, and the source of drinking water and the types of animals around habitation were found to be significantly associated with the presence of Toxoplasma infection (P = 0.002, OR = 2.109 and P = 0.004, OR = 1.693 respectively). The high prevalence among women in third trimester may indicate high risk of congenital infection, and the significant association found between the source of drinking water and infection suggests environmental contamination as a major possible mode of infection. The need to educate pregnant women about the transmission mechanisms of T. gondii, and the effects of the infection on neonates and babies is pertinent in order to effectively control Toxoplasma infection.

  2. New-type spin polarized electron source and its applications; Atarashii spin henkyoku denshi sengen to sono oyo

    Energy Technology Data Exchange (ETDEWEB)

    Saka, T.; Kato, T. [Daido Steel Co. Ltd., Nagoya (Japan); Nakanishi, T.; Okumi, S. [Nagoya University, Nagoya (Japan); Horinaka, H. [Osaka Prefectural University, Osaka (Japan). College of Engineering

    1998-08-20

    This paper reveals that using distorted thin GaAs film can realize high polarization in spin polarized electron ray, and introduces properties of the developed ray source. The paper also touches on the application thereof to property physics. Realization of the high spin polarization is based on use of the `optical polarization method`. With this method, electrons in specific spin state are excited into a conduction band by utilizing the selection law used when valency electrons of zincblende type crystal such as GaAs absorb circular polarization. These electrons are taken out into vacuum and used as polarized electron beams. In order to realize uniformly distorted GaAs film, a method was discussed, with which the thin GaAs films are grown on substrates with different lattice constants, and the films are distorted by means of lattice mismatch. GaAs(1-x)Px was used for the substrates. GaAs(1-x)Px has the lattice constant decrease as the P`s mixed crystal ratio `x` increases. If a thin GaAs film is grown on this substrate, it is possible to obtain GaAs which is subjected to compression stress in the direction parallel with the growing surface, and tensile stress in the vertical direction. 13 refs., 5 figs., 1 tab.

  3. Improved Tet-On and Tet-Off systems for tetracycline-regulated expression of genes in Candida.

    Science.gov (United States)

    Bijlani, Swati; Nahar, Anubhav S; Ganesan, K

    2018-02-01

    Tetracycline-regulated expression of genes is often used for functional analysis of Candida albicans genes. However, the widely used Tet-On system has certain limitations such as prolonged lag time (up to 8 h) for induction and non-uniform expression among the cells. We speculated that poor expression of tetracycline-controlled transactivator (Tet-transactivator) from CaADH1 promoter could be responsible for this, and thus compared the effect of expressing this protein under the control of CaADH1, CaTDH3 and CaRP10 promoters on the expression of GFP from the TET promoter. Only CaRP10 promoter facilitated a more uniform and rapid induction of GFP. However, a high concentration of doxycycline was needed for induction, which is not desirable for assessing certain phenotypes. Tet-Off systems are known to require a low concentration of doxycycline, but a limitation of the widely used Tet-Off system for C. albicans is the use of CaENO1 promoter, which is known to be repressed in the presence of gluconeogenic carbon source, for expression of transactivator. Thus, we have converted the above-mentioned Tet-On systems to Tet-Off systems by site-directed mutagenesis of the Tet-transactivator. Compared to the Tet-On systems, the Tet-Off systems required about 200-fold less concentration of doxycycline for modulation of gene expression. Only the Tet-Off system with CaRP10 promoter driving the expression of transactivator allowed rapid and high level expression of GFP compared to those with CaADH1 or CaTDH3 promoters. The utility of CaRP10 based Tet-On and Tet-Off systems was further validated by the conditional expression of the CaTUP1 gene. We have also adapted these systems for use with Candida tropicalis and find that the Tet-Off system is functional in this species. The Tet systems reported here will be useful for conditional expression of genes in C. albicans as well as C. tropicalis.

  4. ABSTRACT ORAL POETRY OR THE NEGRITUDE OF THE ...

    African Journals Online (AJOL)

    Moto oyo okolinga, akoling yo te'o. Oyo okoboya, akolinga yo mingi o. Oyo okokima, akolanda yo mingi o. Etumbu o, oyo ya ba mpasi ya lamulu o. Le fleuve Zaire. Bateau, o toi bateau ! Te voila emportant mon amour! Sache que j' attends son retour. Le bateau appareille tan~s que mugit la sirene. Le bateau apareille, de ...

  5. Reactive Systems

    DEFF Research Database (Denmark)

    Aceto, Luca; Ingolfsdottir, Anna; Larsen, Kim Guldstrand

    A reactive system comprises networks of computing components, achieving their goals through interaction among themselves and their environment. Thus even relatively small systems may exhibit unexpectedly complex behaviours. As moreover reactive systems are often used in safety critical systems, t...

  6. Data Systems vs. Information Systems

    OpenAIRE

    Amatayakul, Margret K.

    1982-01-01

    This paper examines the current status of “hospital information systems” with respect to the distinction between data systems and information systems. It is proposed that the systems currently existing are incomplete data dystems resulting in ineffective information systems.

  7. Multibody Systems

    DEFF Research Database (Denmark)

    Wagner, Falko Jens

    1999-01-01

    Multibody Systems is one area, in which methods for solving DAEs are of special interst. This chapter is about multibody systems, why they result in DAE systems and what kind of problems that can arise when dealing with multibody systems and formulating their corresponding DAE system.......Multibody Systems is one area, in which methods for solving DAEs are of special interst. This chapter is about multibody systems, why they result in DAE systems and what kind of problems that can arise when dealing with multibody systems and formulating their corresponding DAE system....

  8. Nadzor vazdušnog prostora pasivnim multisenzorskim sistemima / Air surveillance by passive multisensor systems

    Directory of Open Access Journals (Sweden)

    Miljko Erić

    2004-05-01

    Full Text Available Za sprovođenje savremenih operacija, kako na operativno-strategijskom, tako i na taktič kom nivou, izuzetno je važno da se obezbedi kontinuirani nadzor vazdušnog prostora. Poznato je da su aktivni radarski sistemi, na kojima se kod nas trenutno zasniva nadzor vazdušnog prostora, u ratnim uslovima veoma ranjivi i podložni elektronskim i borbenim dejstvima protivnika, što je u toku rata 1999. godine i praktično potvrđeno. Kao alternativa aktivnim radarskim sistemima nameće se koncept pasivnog nadzora vazdušnog prostora korišćenjem distribuiranih multisenzorskih sistema. Predmet širih istraživanja predstavljaju tehnička rešenja integrisanog multisenzorskog sistema za pasivan nadzor vazdušnog prostora, koji se sastoji od sledećih podsistema za nadzor vazdušnog prostora: na radio-gonimetarskom principu u frekvencijskom opsegu 20-3000 MHz, na zvukometrijskom principu, na principu vizuelnih osmatračkih stanica, na termovizijskom i televizijskom principu. U ovom radu analizirani su opšti principi pasivnog nadzora vazdušnog prostora multisenzorskim sistemima i predložena je tehnička koncepcija radio-goniometarskog i zvukometrijskog podsistema. / It is very important to provide continuous air surveillance in to realize modern operations on the operational-strategic level and tactical, as well. It is well known that active radar systems, on which air surveillance is based in our country, are very vulnerable in war times and susceptible to the electronic counter and combat attacks of the enemy, what is shown practically in 1999 war. Air surveillance by passive multi-sensor systems is a possible alternative to the air surveillance by the active radar systems. Subjects of our wider research are technical solutions of integrated multi-sensor systems for passive air surveillance which consists of the following subsystems: a subsystem for the air communication intelligence within the frequency range 20-3000 MHz based on intercept

  9. Lymph system

    Science.gov (United States)

    Lymphatic system ... neck, under the arms, and groin. The lymph system includes the: Tonsils Adenoids Spleen Thymus ... JE, Flynn JA, Solomon BS, Stewart RW. Lymphatic system. In: Ball JW, Dains JE, Flynn JA, Solomon ...

  10. Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.

    Science.gov (United States)

    Viedma-Rodríguez, Rubí; Martínez-Hernández, María Guadalupe; Flores-López, Luis Antonio; Baiza-Gutman, Luis Arturo

    2018-01-01

    Obesity and type II diabetes mellitus have contributed to the increase of breast cancer incidence worldwide. High glucose concentration promotes the proliferation of metastatic cells, favoring the activation of the plasminogen/plasmin system, thus contributing to tumor progression. The efficient formation of plasmin is dependent on the binding of plasminogen to the cell surface. We studied the effect of ε-aminocaproic acid (EACA), an inhibitor of the binding of plasminogen to cell surface, on proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and plasminogen activation system, in metastatic MDA-MB-231 breast cancer cells grown in a high glucose microenvironment and treated with insulin. MDA-MB-231 cells were treated with EACA 12.5 mmol/L under high glucose 30 mmol/L (HG) and high glucose and insulin 80 nmol/L (HG-I) conditions, evaluating: cell population growth, % of viability, migratory, and invasive abilities, as well as the expression of uPA, its receptor (uPAR), and its inhibitor (PAI-1), by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, MMP-2 and MMP-9 mRNAs were evaluated by RT-PCR. Markers of EMT were evaluated by Western blot. Additionally, the presence of active uPA was studied by gel zymography, using casein-plasminogen as substrates. EACA prevented the increase in cell population, migration and invasion induced by HG and insulin, which was associated with the inhibition of EMT and the attenuation of HG- and insulin-dependent expression of uPA, uPAR, PAI-1, MMP-2, MMP-9, α-enolase (ENO A), and HCAM. The interaction of plasminogen to the cell surface and plasmin formation are mediators of the prometastasic action of hyperglycemia and insulin, potentially, EACA can be employed in the prevention and as adjuvant treatment of breast tumorigenesis promoted by hyperglycemia and insulin.

  11. Operating Systems

    Indian Academy of Sciences (India)

    computer networks and also in Internet security concepts. Keywords. Operating systems, file sys- tems, time-shared systems. GENERAL I ARTICLE. Operating Systems. 1. Objectives and Evolution. M Suresh Babu. In this article we examine the objectives and fun.ctions of operating systems, and then we trace the evolution ...

  12. Bitcoin System

    OpenAIRE

    Jan Lánský

    2017-01-01

    Cryptocurrency systems are purely digital and decentralized systems that use cryptographic principles to confirm transactions. Bitcoin is the first and also the most widespread cryptocurrency. The aim of this article is to introduce Bitcoin system using a language understandable also to readers without computer science education. This article captures the Bitcoin system from three perspectives: internal structure, network and users. Emphasis is placed on brief and clear definitions (system co...

  13. Reactive Systems

    DEFF Research Database (Denmark)

    Aceto, Luca; Ingolfsdottir, Anna; Larsen, Kim Guldstrand

    A reactive system comprises networks of computing components, achieving their goals through interaction among themselves and their environment. Thus even relatively small systems may exhibit unexpectedly complex behaviours. As moreover reactive systems are often used in safety critical systems......, the need for mathematically based formal methodology is increasingly important. There are many books that look at particular methodologies for such systems. This book offers a more balanced introduction for graduate students and describes the various approaches, their strengths and weaknesses, and when...

  14. Systems effectiveness

    CERN Document Server

    Habayeb, A R

    1987-01-01

    Highlights three principal applications of system effectiveness: hardware system evaluation, organizational development and evaluation, and conflict analysis. The text emphasizes the commonality of the system effectiveness discipline. The first part of the work presents a framework for system effectiveness, partitioning and hierarchy of hardware systems. The second part covers the structure, hierarchy, states, functions and activities of organizations. Contains an extended Appendix on mathematical concepts and also several project suggestions.

  15. Bitcoin System

    Directory of Open Access Journals (Sweden)

    Jan Lánský

    2017-06-01

    Full Text Available Cryptocurrency systems are purely digital and decentralized systems that use cryptographic principles to confirm transactions. Bitcoin is the first and also the most widespread cryptocurrency. The aim of this article is to introduce Bitcoin system using a language understandable also to readers without computer science education. This article captures the Bitcoin system from three perspectives: internal structure, network and users. Emphasis is placed on brief and clear definitions (system components and their mutual relationships. A new system view of the stated terms constitutes author’s own contribution.

  16. Biliary system

    Science.gov (United States)

    The biliary system creates, moves, stores, and releases bile into the duodenum . This helps the body digest food. It also assists ... from the liver to the duodenum. The biliary system includes: The gallbladder Bile ducts and certain cells ...

  17. Intelligent Systems

    Data.gov (United States)

    National Aeronautics and Space Administration — The autonomous systems (AS) project, led by NASA Ames, is developing software for system operation automation. AS technology will help astronauts make more decisions...

  18. Systems thinking.

    Science.gov (United States)

    Cabrera, Derek; Colosi, Laura; Lobdell, Claire

    2008-08-01

    Evaluation is one of many fields where "systems thinking" is popular and is said to hold great promise. However, there is disagreement about what constitutes systems thinking. Its meaning is ambiguous, and systems scholars have made diverse and divergent attempts to describe it. Alternative origins include: von Bertalanffy, Aristotle, Lao Tsu or multiple aperiodic "waves." Some scholars describe it as synonymous with systems sciences (i.e., nonlinear dynamics, complexity, chaos). Others view it as taxonomy-a laundry list of systems approaches. Within so much noise, it is often difficult for evaluators to find the systems thinking signal. Recent work in systems thinking describes it as an emergent property of four simple conceptual patterns (rules). For an evaluator to become a "systems thinker", he or she need not spend years learning many methods or nonlinear sciences. Instead, with some practice, one can learn to apply these four simple rules to existing evaluation knowledge with transformative results.

  19. Declarative Systems

    OpenAIRE

    Condie, Tyson

    2011-01-01

    Building system software is a notoriously complex and arduous endeavor.Developing tools and methodologies for practical system software engineeringhas long been an active area of research. This thesis explores system softwaredevelopment through the lens of a declarative, data-centric programminglanguage that can succinctly express high-level system specifications and bedirectly compiled to executable code. By unifying specification andimplementation, our approach avoids the common problem o...

  20. Reactive Systems

    DEFF Research Database (Denmark)

    Aceto, Luca; Ingolfsdottir, Anna; Larsen, Kim Guldstrand

    A reactive system comprises networks of computing components, achieving their goals through interaction among themselves and their environment. Thus even relatively small systems may exhibit unexpectedly complex behaviours. As moreover reactive systems are often used in safety critical systems, t...... into account. The book has arisen from various courses taught in Denmark and Iceland and is designed to give students a broad introduction to the area, with exercises throughout....

  1. Watchdog System

    DEFF Research Database (Denmark)

    Madsen, Tanja Kidholm Osmann; Bahnsen, Chris Holmberg; Jensen, Morten Bornø

    This deliverable is part of WP4. Overall WP4 is motivated by the need for automatic systems that can ease the task of annotating massive amounts of traffic data. Concretely this deliverable is related to WP4.2 - the watchdog system. The idea with the watchdog is to develop a system that can remove...

  2. Retrofitting Systems

    DEFF Research Database (Denmark)

    Rose, Jørgen

    1997-01-01

    This report gives an overview of the different retrofitting possibilities that are available today. The report looks at both external and internal systems for external wall constructions, roof constructions, floor constructions and foundations. All systems are described in detail in respect to use...... and methods, and the efficiency of the different systems are discussed....

  3. systemic matching

    African Journals Online (AJOL)

    IICBA01

    understanding of scientific concepts,(iv) converted from surface learning to deep learning of chemistry concepts and chemical processes,(v) enhance their abilities to construction, or analysis of a novel systemic diagrams using chemical information, (vi) develop their thinking skills towards systemic thinking. Types Systemic ...

  4. Systems Engineering

    Science.gov (United States)

    Pellerano, Fernando

    2015-01-01

    This short course provides information on what systems engineering is and how the systems engineer guides requirements, interfaces with the discipline leads, and resolves technical issues. There are many system-wide issues that either impact or are impacted by the thermal subsystem. This course will introduce these issues and illustrate them with real life examples.

  5. Operating systems

    CERN Document Server

    Tsichritzis, Dionysios C; Rheinboldt, Werner

    1974-01-01

    Operating Systems deals with the fundamental concepts and principles that govern the behavior of operating systems. Many issues regarding the structure of operating systems, including the problems of managing processes, processors, and memory, are examined. Various aspects of operating systems are also discussed, from input-output and files to security, protection, reliability, design methods, performance evaluation, and implementation methods.Comprised of 10 chapters, this volume begins with an overview of what constitutes an operating system, followed by a discussion on the definition and pr

  6. Induction of PDE5 and de-sensitization to endogenous NO signaling in a systemic resistance artery under altered blood flow

    Science.gov (United States)

    Zhang, Haiying; Pakeerappa, Praveen; Jae Lee, Hyon; Fisher, Steven A.

    2009-01-01

    In the classical pathway, the opposing activities of guanylyl cyclases (GC) and phosphodiesterases (PDE), and the effect of the cGMP-dependent protein kinase (cGK) on its targets, determine the biological responses to NO signaling. Here we tested the hypothesis that vascular dysfunction may be due to altered expression and activity of these effectors of NO signaling. Every other set of rat second order mesenteric resistance arteries (MA) were ligated, resulting in chronic low flow (LF) in the upstream MA1 and high flow (HF) in the adjacent MA1 without tissue ischemia. eNOS and iNOS were up-regulated in HF and LF MA1, respectively, in the sub-acute phase (four days) of vascular remodeling. The Day4 HF/LF MA1s were under increased control of NO as indicated by reduced sensitivity to the vasoconstrictor phenylephrine and its normalization with the NOS antagonist L-NAME. PDE5 mRNA and protein were also significantly up-regulated in the HF/LF MA1 with no change in sGC or PKG1, an effect that was dependent upon NO synthesis. The PDE5 inhibitor Sildenafil was several-fold more powerful in relaxing the HF/LF MA1s, and pre-treatment with Sildenafil uncovered an increased responsiveness of HF/LF MA1s to the NO donor DEA/NO. We conclude that induction of PDE5 de-sensitizes this systemic resistance artery to sustained NO signaling under chronic HF/LF. Treatment with PDE5 antagonists, in contrast to NO donors, may more specifically and effectively increase blood flow to chronically hypo-perfused tissues. PMID:19374906

  7. Protective actions of progesterone in the cardiovascular system: potential role of membrane progesterone receptors (mPRs) in mediating rapid effects.

    Science.gov (United States)

    Thomas, Peter; Pang, Yefei

    2013-06-01

    The protective functions of progesterone in the cardiovascular system have received little attention even though evidence has accumulated that progesterone lowers blood pressure, inhibits coronary hyperactivity and has powerful vasodilatory and natriuretic effects. One possible reason why potential beneficial actions of progesterone on cardiovascular functions have not been extensively studied is that divergent effects to those of progesterone have been observed in many clinical trials with synthetic progestins such as medroxyprogesterone acetate which are associated with increased risk of coronary disease. Evidence that progesterone exerts protective effects on cardiovascular functions is briefly reviewed. The finding that progesterone administration decreases blood vessel vasoconstriction in several animal models within a few minutes suggests that rapid, nongenomic progesterone mechanisms are of physiological importance in regulating vascular tone. Rapid activation of second messenger pathways by progesterone has been observed in vascular endothelial and smooth muscle cells, resulting in alterations in endothelial nitric oxide synthase (eNOS) activity and calcium influx, respectively. Both nuclear progesterone receptors (PRs) and novel membrane progesterone receptors (mPRs) are candidates for the intermediaries in these rapid, cell-surface initiated progesterone actions in endothelial and smooth muscle vascular cells. PRs have been detected in both cell types. New data are presented showing mPRα, mPRβ and mPRγ are also present in human endothelial and smooth muscle vascular cells. Preliminary evidence suggests mPRs mediate rapid progestin signaling in these endothelial cells, resulting in down-regulation of cAMP production and increased nitric oxide synthesis. The role of mPRs in progesterone regulation of cardiovascular functions warrants further investigation. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. Expert System

    DEFF Research Database (Denmark)

    Hildebrandt, Thomas Troels; Cattani, Gian Luca

    2016-01-01

    An expert system is a computer system for inferring knowledge from a knowledge base, typically by using a set of inference rules. When the concept of expert systems was introduced at Stanford University in the early 1970s, the knowledge base was an unstructured set of facts. Today the knowledge...... base of expert systems is often given in terms of an ontology, extracted and built from various data sources by employing natural language-processing and statistics. To emphasize such capabilities, the term “expert” is now often replaced by “cognitive,” “knowledge,” “knowledge-based,” or “intelligent......” system. With very few exceptions, general-purpose expert systems have failed to emerge so far. However, expert systems are applied in specialized domains, particularly in healthcare. The increasing availability of large quantities of data to organizations today provides a valuable opportunity...

  9. Recommender systems

    CERN Document Server

    Kembellec, Gérald; Saleh, Imad

    2014-01-01

    Acclaimed by various content platforms (books, music, movies) and auction sites online, recommendation systems are key elements of digital strategies. If development was originally intended for the performance of information systems, the issues are now massively moved on logical optimization of the customer relationship, with the main objective to maximize potential sales. On the transdisciplinary approach, engines and recommender systems brings together contributions linking information science and communications, marketing, sociology, mathematics and computing. It deals with the understan

  10. Systemic Assurance

    Science.gov (United States)

    2015-07-31

    benefits. The first is direct: Cost- effective and rapid recertification is essential to support the development of systems that must adapt to changes...simulations, cyber-physical robotic systems, and extremely large commercial Java programs. An important goal is to develop incrementally compostable ...combinations of models, practices, and tools for obtaining the most cost- and schedule- effective combinations for the assurance of necessary system

  11. Systems Engineering

    OpenAIRE

    Vaughan, William W.

    2016-01-01

    The term “systems engineering” when entered into the Google search page, produces a significant number of results, evidence that systems engineering is recognized as being important for the success of essentially all products. Since most readers of this item will be rather well versed in documents concerning systems engineering, I have elected to share some of the points made on this subject in a document developed by the European Cooperation for Space Standardization (ECSS), a component of t...

  12. Geothermal systems

    Science.gov (United States)

    Mohl, C.

    1978-01-01

    Several tasks of JPL related to geothermal energy are discussed. The major task is the procurement and test and evaluation of a helical screw drive (wellhead unit). A general review of geothermal energy systems is given. The presentation focuses attention on geothermal reservoirs in California, with graphs and charts to support the discussion. Included are discussions on cost analysis, systems maintenance, and a comparison of geothermal and conventional heating and cooling systems.

  13. Systemic darwinism.

    Science.gov (United States)

    Winther, Rasmus Grønfeldt

    2008-08-19

    Darwin's 19th century evolutionary theory of descent with modification through natural selection opened up a multidimensional and integrative conceptual space for biology. We explore three dimensions of this space: explanatory pattern, levels of selection, and degree of difference among units of the same type. Each dimension is defined by a respective pair of poles: law and narrative explanation, organismic and hierarchical selection, and variational and essentialist thinking. As a consequence of conceptual debates in the 20th century biological sciences, the poles of each pair came to be seen as mutually exclusive opposites. A significant amount of 21st century research focuses on systems (e.g., genomic, cellular, organismic, and ecological/global). Systemic Darwinism is emerging in this context. It follows a "compositional paradigm" according to which complex systems and their hierarchical networks of parts are the focus of biological investigation. Through the investigation of systems, Systemic Darwinism promises to reintegrate each dimension of Darwin's original logical space. Moreover, this ideally and potentially unified theory of biological ontology coordinates and integrates a plurality of mathematical biological theories (e.g., self-organization/structure, cladistics/history, and evolutionary genetics/function). Integrative Systemic Darwinism requires communal articulation from a plurality of perspectives. Although it is more general than these, it draws on previous advances in Systems Theory, Systems Biology, and Hierarchy Theory. Systemic Darwinism would greatly further bioengineering research and would provide a significantly deeper and more critical understanding of biological reality.

  14. Intelligent systems

    CERN Document Server

    Irwin, J David

    2011-01-01

    Technology has now progressed to the point that intelligent systems are replacing humans in the decision making processes as well as aiding in the solution of very complex problems. In many cases intelligent systems are already outperforming human activities. Artificial neural networks are not only capable of learning how to classify patterns, such images or sequence of events, but they can also effectively model complex nonlinear systems. Their ability to classify sequences of events is probably more popular in industrial applications where there is an inherent need to model nonlinear system

  15. Differential systems

    CERN Document Server

    Thomas, Joseph Miller

    1937-01-01

    The main goal of this book is to present the theory of systems of partial differential equations and the theory of Pfaffian systems so as to exhibit clearly the relations between them. In presenting the theory of Pfaffian systems, the author develops, in detail, the theories of Grassmann algebras and rings with differentiation. In particular, following Grassmann and É. Cartan, he introduces and freely uses what is now known as a ring of differential forms with functional coefficients. In presenting the theory of systems of partial differential equations, the author concentrates on the existenc

  16. Energetic Systems

    Data.gov (United States)

    Federal Laboratory Consortium — The Energetic Systems Division provides full-spectrum energetic engineering services (project management, design, analysis, production support, in-service support,...

  17. Design and erection of the MUSEUM BRIDGE; Bijutsu no sekai eno yume no kakehashi. MUSEUM BRIDGE no sekkei seko

    Energy Technology Data Exchange (ETDEWEB)

    Ishihara, S.; Tsunekawa, M.; Umda, A.; Kishimoto, Y.; Yotsukura, Y.; Miitsu, K.; Okada, S.; Furukawa, M. [Kawasaki Heavy Industries, Ltd., Kobe (Japan)

    1998-12-20

    MUSEUM Projects for the religious corporation of Shinji-Shumeikai, at Shigaraki, Shiga prefecture. Fundamental design was by a project team led by the world-famous architect I.M. Pei. The museum is located in a prefectural park surrounded by forests. The design concept of the bridge aimed to connect Shangri-La (museum) to the real world. In order to satisfy this purpose, a cable-stayed bridge with external tendon was adopted as an original structure style, and details such as handrails, gratings, and gloss painting taken into consideration. The authors report the characteristics of the bridge and the sequence of erection. (author)

  18. Shear stress affects the intracellular distribution of eNOS: Direct demonstration by a novel in vivo technique

    NARCIS (Netherlands)

    C. Cheng (Caroline (Ka Lai)); M.J. van Haperen (Rien); M.C. de Waard (Monique); L.C.A. van Damme (Luc); D. Tempel (Dennie); R. Hanemaaijer (Roeland); W.A. van Cappellen (Gert); J.A. Bos (Joop); C.J. Slager (Cornelis); D.J.G.M. Duncker (Dirk); A.F.W. van der Steen (Ton); M.P.G. de Crom (Rini); R. Krams (Rob)

    2005-01-01

    textabstractThe focal location of atherosclerosis in the vascular tree is correlated with local variations in shear stress. We developed a method to induce defined variations in shear stress in a straight vessel segment of a mouse. To this end, a cylinder with a tapered lumen was placed around the

  19. Role of iNOS and eNOS expression in a group of Egyptian diabetic and nondiabetic nephropathy patients

    Directory of Open Access Journals (Sweden)

    Mayssa I. Aly

    2013-01-01

    Conclusion The presence of iNOS is associated with tubular damage resulting in renal failure. The upregulation of NO in diabetes mellitus type 2 may explain the endothelial dysfunction that is associated with almost all diabetic complications.

  20. Phenomena in molten aluminium induced by silica particles addition. Aluminium yotochu eno silica ryushi tenka ni yotte hikiokosareru shogensho

    Energy Technology Data Exchange (ETDEWEB)

    Tsujikawa, M.; Hashimoto, T.; Kawamoto, M. (University of Osaka Prefecture, Osaka (Japan). College of Engineering)

    1992-08-25

    Fundamental tests were performed for the purpose of utilizing thermit reaction, which was induced in metal liquid phase, for making new material. Exothermic reaction caused by the thermit reaction was observed when coarse silica particles were added while stirring to the Al-Si eutectic melt remaining at 1023K. Silica particles were reduced into Si in a short time, and dissolved in the melt. As a result of the thermit reaction, [alpha]-alumina particles which were as large as added silica particles were generated in the melt. Large primary Si and alumina particles as well as Al-Si eutectic matrix were observed within microstructures of the specimens after solidification. When Mg was added prior to the addition of silica particles, the dispersion of silica particles was promoted and the thermit reaction was more accelerated. Therefore the highest temperature of the melt attained by this reaction was higher than that attained by the reaction without Mg addition. Moreover, the analysis of generated oxide particles proved that the thermit reaction started from outside of silica particles. 7 refs., 13 figs., 1 tab.

  1. Estimation of the charge quantity from solar cell to battery; Taiyo denchi ni yoru chikudenchi eno juden yosoku

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, K.; Nishitani, M. [Daiichi University, College of Technology, Kagoshima (Japan)

    1996-10-27

    In performing an experiment of running a small electric vehicle by installing solar cells in it and by charging the storage battery at a specific voltage, an estimation was made on the charge quantity to the battery for each solar altitude and inclination of a module at different hours. The solar altitude was determined at Daiichi University, College of Technology, according to the month and the time of day from a formula using day-declination and time equation of a chronological table of science. The quantity of global solar radiation was determined by resolving the solar radiation into its direct and diffuse components on the basis of the extra-terrestrial solar radiation quantity with the change in radius vector taken into consideration; and then, the global solar radiation on the inclined face was obtained from the angle of inclination and incidence. On the roof of a Daiichi University building, solar cell modules were installed facing north and south at 0{degree}, 30{degree}, 45{degree}, 60{degree} and 90{degree} each, so that a short-circuit current was measured for each differently inclined angle. As a result of the experiment, shown in an regression formula is a relation between the temperature conversion value of the maximum output of the solar cell at the standard temperature of 25{degree}C and the quantity of solar radiation on the inclined surface. Consequently, it enabled the prediction of a charging quantity, in the case of running a small vehicle with solar cells installed, from the quantity of solar radiation on the inclined surface in the clear weather. 2 refs., 4 figs., 2 tabs.

  2. Erythropoietin increases bioavailability of tetrahydrobiopterin and protects cerebral microvasculature against oxidative stress induced by eNOS uncoupling.

    Science.gov (United States)

    Santhanam, Anantha Vijay R; d'Uscio, Livius V; Katusic, Zvonimir S

    2014-11-01

    This study was designed to determine whether treatment with erythropoietin (EPO) could protect cerebral microvasculature against the pathological consequences of endothelial nitric oxide (NO) synthase uncoupling. Wild-type and GTP cyclohydrolase I (GTPCH-I)-deficient hph1 mice were administered EPO (1000 U/kg/day, s.c., 3 days). Cerebral microvessels of hph1 mice demonstrated reduced tetrahydrobiopterin (BH4) bioavailability, increased production of superoxide anions and impaired endothelial NO signaling. Treatment of hph1 mice with EPO attenuated the levels of 7,8-dihydrobiopterin, the oxidized product of BH4, and significantly increased the ratio of BH4 to 7,8-dihydrobiopterin. Moreover, EPO decreased the levels of superoxide anions and increased NO bioavailability in cerebral microvessels of hph1 mice. Attenuated oxidation of BH4 and inhibition of endothelial NO synthase uncoupling were explained by the increased expression of antioxidant proteins, manganese superoxide dismutase, and catalase. The protective effects of EPO observed in cerebral microvessels of hph1 mice were also observed in GTPCH-I siRNA-treated human brain microvascular endothelial cells exposed to EPO (1 U/mL or 10 U/mL; 3 days). Our results suggest that EPO might protect the neurovascular unit against oxidative stress by restoring bioavailability of BH4 and endothelial NO in the cerebral microvascular endothelium. We demonstrate that treatment with erythropoietin (EPO) could protect cerebral microvasculature against the pathological consequences of endothelial nitric oxide (NO) synthase uncoupling. Our results suggest that EPO might protect the neurovascular unit against oxidative stress by restoring bioavailability of tetrahydrobiopterin (BH4) and endothelial nitric oxide. © 2014 International Society for Neurochemistry.

  3. Ilves viskas koos Bedwettersiga näppu. President Ilves kohtus õpilaste ja kaitseliitlastega / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Münchenis MTV Euroopa muusikaauhindade jagamisel Uue Euroopa Heli kategoorias lauluga "Dramatic Letter to Conscience"esikoha võitnud Pärnu pop-punkbändist Bedwetters ja president Toomas Hendrik Ilvese Pärnu Sütevaka humanitaargümnaasiumi ja Kaitseliidu Pärnumaa maleva külastusest 9. novembril

  4. Study about the effect of microgravity on biofunctions; Seitai kino eno bisho juryoku no eikyo ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    A study is made for the elucidation of the effect of microgravity on biofunctions. The protoplast of shiitake is exposed to microgravity and then cultured, and a significant difference occurs. The rate of colony formation by cell fusion in the test sector is found to be two times higher than that in the control sector. When swimming ciliates which are unicellular are suddenly exposed to microgravity, their swimming speeds changed differently according to the direction of swimming. When a mouse subjected to acupuncture for three days is exposed to microgravity, its water metabolism and excretory function are enhanced. A mouse treated with Chinese medicine reacts in the similar way. The change due to microgravity in the amount of acetylcholine in the hypothalamus is found characteristically time dependent. Mice infected with lethal herpes viruses just after exposure to microgravity die at a rate in proportion to the number of exposures and to the amount of viruses given. The migration speed of chemical stripes in case of a gel-base BZ (Belousov-Zhabotinsky) reaction under microgravity is equal to that on the ground. The trigger wave migration speed in case of a water solution-base BZ reaction under microgravity is reduced to approximately 80% of the speed on the ground. 12 refs., 31 figs., 3 tabs.

  5. Efficacy of nitric oxide, with or without continuing antihypertensive treatment, for management of high blood pressure in acute stroke (ENOS)

    DEFF Research Database (Denmark)

    Bath, Philip M W; Woodhouse, Lisa; Scutt, Polly

    2015-01-01

    BACKGROUND: High blood pressure is associated with poor outcome after stroke. Whether blood pressure should be lowered early after stroke, and whether to continue or temporarily withdraw existing antihypertensive drugs, is not known. We assessed outcomes after stroke in patients given drugs......), started within 48 h of stroke onset, or to no glyceryl trinitrate (control group). A subset of patients who were taking antihypertensive drugs before their stroke were also randomly assigned to continue or stop taking these drugs. The primary outcome was function, assessed with the modified Rankin Scale......), and was significantly reduced on day 1 in 2000 patients allocated to glyceryl trinitrate compared with 2011 controls (difference -7·0 [95% CI -8·5 to -5·6] mm Hg/-3·5 [-4·4 to -2·6] mm Hg; both pantihypertensive drugs compared with 1044 patients randomised...

  6. Report to the Nuclear Regulatory Commission from the staff panel on the Commission's determination of an Extraordinary Nuclear Occurrence (ENO)

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-01-01

    The Panel finds that the first criterion, pertaining to whether the accident caused a discharge of radioactive material or levels of radiation offsite as defined in 10 CFR 140.84, has not been met. It further finds that there is presently insufficient information to support any definitive finding as to whether or not the second criterion, relating to damage to persons or property offsite as defined in 10 CFR 140.85, has been met. Since the Panel has not found that both criteria have been met, it recommends that the Commission determine that the accident at Three Mile Island did not constitute an extraordinary nuclear occurrence.

  7. Port Artur 3 arhitektuurikonkursi võidutöö sündis Itaalias / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2006-01-01

    Pärnu kesklinna jõeäärse ala rahvusvahelise arhitektuurikonkursi tulemustest. Võitis itaalia arhitektide projekt "Flood". Teise koha võitis projekt "Istu ja vaata" (Jan Skolimovski ja Peeter Loo), kolmanda koha "Nicesaar" (Ott Kadarik, Villem Tomiste, Mihkel Tüür)

  8. Inhibition of GTP-dependent vesicle trafficking impairs internalization of plasmalemmal eNOS and cellular nitric oxide production

    National Research Council Canada - National Science Library

    Chatterjee, Suvro; Cao, Sheng; Peterson, Timothy E; Simari, Robert D; Shah, Vijay

    2003-01-01

    .... This was associated with the mobilization of eNOS-GFP protein into Triton X-100-insoluble fractions of cell lysates, and an internalization of plasmalemmal eNOS-GFP in live and fixed ECV 304 cells...

  9. Projektovanje geoinformacionog sistema za potrebe elektrodistributivnog preduzeća ''Elektro-Bijeljina'' u Bijeljini : Projecting of geoinformation system in service of electro-distributive firm ''Elektro-Bijeljina'' in Bijeljina

    Directory of Open Access Journals (Sweden)

    Zoran Perić

    2011-03-01

    Full Text Available U radu je opisani relevantni aspekti za razvoj i primjenu geoinformacionog sistema (GIS u elektrodistributivnom preduzeću ''Elektro-Bijeljina'' u Bijeljini. U kontekstu izrade projekta GIS-a izvršeno je poređenje pojedinih segmenata poslovanja interesantnih za njegovu primjenu. Razrađen je pristup projektovanja geoinformacionog sistema kroz sagledavanje konkretnih problema vezanih za nedostatak međusobne interakcije uposlenika u smislu kvalitetne međusobne komunikacije, pravovremenog informisanja i izvršavanja kancelarijskih i terenskih zadataka. Obzirom da nepravovremena i/ili netačna informacija može dovesti do usporavanja ili potpunog zaustavljanja određene poslovne procedure, za realizaciju projekta GIS-a su ovi problemi prepoznati kao glavni izazov i slabost koju ovakav sistem treba reducirati. : In this paper possibility of development and use of geo/information system (GIS was described in the service of electro distributive firm “Elektro-Bijeljina” in Bijeljina. In the context of creating the project, from an economic aspect, certain segments of business were compared, especially those in which space information system can be utilized. Problems related to mutual interaction of employees were analyzed, such as a lack of qualitative mutual communication, timely informing and accomplishing office and field assignments. Considering the fact that wrong or ill-timed information can cause a slowdown or a complete block of a certain procedure, project GIS finds these problems as the main challenge, and disadvantage that needs to be reduced.

  10. Anticipatory systems as linguistic systems

    Science.gov (United States)

    Ekdahl, Bertil

    2000-05-01

    The idea of system is well established although not well defined. What makes up a system depends on the observer. Thinking in terms of systems is only a convenient way to conceptualize organizations, natural or artificial, that show coherent properties. Among all properties, which can be ascribed to systems, one property seems to be more outstanding than others, namely that of being anticipatory. In nature, anticipatory properties are found only in living organizations. In this way it can be said to separate non-living systems from living because there is no indication that any natural phenomenon occurring in systems where there is no indication of life is anticipatory. The characteristic of living systems is that they are exposed to the evolution contrary to causal systems that do not undergo changes due to the influence of the environment. Causal systems are related to the past in such a way that subsequent situations can be calculated from knowledge of past situations. In causal systems the past is the cause of the present and there is no reference to the future as a determining agent, contrary to anticipatory systems where expectations are the cause of the present action. Since anticipatory properties are characteristic of living systems, this property, as all other properties in living systems, is a result of the evolution and can be found in plants as well as in animals. Thus, it is not only tied to consciousness but is found at a more basic level, i.e., in the interplay between genotype and phenotype. Anticipation is part of the genetic language in such a way that appropriate actions, for events in the anticipatory systems environment, are inscribed in the genes. Anticipatory behavior, as a result of the interpretation of the genetic language, has been selected by the evolution. In this paper anticipatory systems are regarded as linguistic systems and I argue that as such anticipation cannot be fragmented but must be holistically studied. This has the

  11. Operating Systems

    Indian Academy of Sciences (India)

    The process concept and concurrency are at the heart of modern operating systems (OS). A process is the unit of work in a computer system. A process must be in main memory during execution. To improve the utilization of central processing unit. (CPU) as well as the speed of its response to its users, the computer must ...

  12. Embedded Systems

    Indian Academy of Sciences (India)

    An embedded system is a microprocessor-based system that is incorporated into a device to monitor and control the functions of the components of the device. They are used in many devices ranging from a microwave oven to a nuclear reactor. Unlike personal computers that run a variety of applications, embedded.

  13. Power system

    Science.gov (United States)

    Hickam, Christopher Dale [Glasford, IL

    2008-03-18

    A power system includes a prime mover, a transmission, and a fluid coupler having a selectively engageable lockup clutch. The fluid coupler may be drivingly connected between the prime mover and the transmission. Additionally, the power system may include a motor/generator drivingly connected to at least one of the prime mover and the transmission. The power-system may also include power-system controls configured to execute a control method. The control method may include selecting one of a plurality of modes of operation of the power system. Additionally, the control method may include controlling the operating state of the lockup clutch dependent upon the mode of operation selected. The control method may also include controlling the operating state of the motor/generator dependent upon the mode of operation selected.

  14. Reactive Systems

    DEFF Research Database (Denmark)

    Aceto, Luca; Ingolfsdottir, Anna; Larsen, Kim Guldstrand

    A reactive system comprises networks of computing components, achieving their goals through interaction among themselves and their environment. Thus even relatively small systems may exhibit unexpectedly complex behaviours. As moreover reactive systems are often used in safety critical systems, t...... into account. The book has arisen from various courses taught in Denmark and Iceland and is designed to give students a broad introduction to the area, with exercises throughout......., the need for mathematically based formal methodology is increasingly important. There are many books that look at particular methodologies for such systems. This book offers a more balanced introduction for graduate students and describes the various approaches, their strengths and weaknesses, and when...... they are best used. Milner's CCS and its operational semantics are introduced, together with the notions of behavioural equivalences based on bisimulation techniques and with recursive extensions of Hennessy-Milner logic. In the second part of the book, the presented theories are extended to take timing issues...

  15. Facial nerve paralysis associated with temporal bone masses.

    Science.gov (United States)

    Nishijima, Hironobu; Kondo, Kenji; Kagoya, Ryoji; Iwamura, Hitoshi; Yasuhara, Kazuo; Yamasoba, Tatsuya

    2017-10-01

    To investigate the clinical and electrophysiological features of facial nerve paralysis (FNP) due to benign temporal bone masses (TBMs) and elucidate its differences as compared with Bell's palsy. FNP assessed by the House-Brackmann (HB) grading system and by electroneurography (ENoG) were compared retrospectively. We reviewed 914 patient records and identified 31 patients with FNP due to benign TBMs. Moderate FNP (HB Grades II-IV) was dominant for facial nerve schwannoma (FNS) (n=15), whereas severe FNP (Grades V and VI) was dominant for cholesteatomas (n=8) and hemangiomas (n=3). The average ENoG value was 19.8% for FNS, 15.6% for cholesteatoma, and 0% for hemangioma. Analysis of the correlation between HB grade and ENoG value for FNP due to TBMs and Bell's palsy revealed that given the same ENoG value, the corresponding HB grade was better for FNS, followed by cholesteatoma, and worst in Bell's palsy. Facial nerve damage caused by benign TBMs could depend on the underlying pathology. Facial movement and ENoG values did not correlate when comparing TBMs and Bell's palsy. When the HB grade is found to be unexpectedly better than the ENoG value, TBMs should be included in the differential diagnosis. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Saturn Systems.

    Science.gov (United States)

    U Rehman, Habib; McKee, Nida A; McKee, Michael L

    2016-01-15

    Several ring systems (Saturn systems) have been studied using DFT methods that include dispersion effects. Comparison with X-ray structures are made with three systems, and the agreement is quite good. Binding enthalpies and binding free energies in dichloromethane and toluene have been computed. The effect of an encapsulated lithium cation is accessed by comparing C60 @(C6 H4 )10 and [Li@C60 @(C6 H4 )10 ](+). The [Li@C60 ](+) cation is a much better acceptor than C60 which leads to greater donor-acceptor interactions and larger charge transfer from the ring to [Li@C60 ](+). © 2015 Wiley Periodicals, Inc.

  17. Dynamical systems

    CERN Document Server

    Sternberg, Shlomo

    2010-01-01

    Celebrated mathematician Shlomo Sternberg, a pioneer in the field of dynamical systems, created this modern one-semester introduction to the subject for his classes at Harvard University. Its wide-ranging treatment covers one-dimensional dynamics, differential equations, random walks, iterated function systems, symbolic dynamics, and Markov chains. Supplementary materials offer a variety of online components, including PowerPoint lecture slides for professors and MATLAB exercises.""Even though there are many dynamical systems books on the market, this book is bound to become a classic. The the

  18. Physical Systems

    CERN Document Server

    Belkind, Ori

    2012-01-01

    Based on the concept of a physical system, this book offers a new philosophical interpretation of classical mechanics and the Special Theory of Relativity. According to Belkind's view the role of physical theory is to describe the motions of the parts of a physical system in relation to the motions of the whole. This approach provides a new perspective into the foundations of physical theory, where motions of parts and wholes of physical systems are taken to be fundamental, prior to spacetime, material properties and laws of motion. He defends this claim with a constructive project, deriving b

  19. Pravci razvoja automatizacije informacionog sistema održavanja u vojsci Srbije i Crne Gore / Automatizacion development directions of maintenance information system in the military forces of Serbia and Montenegro

    Directory of Open Access Journals (Sweden)

    Saša Veselinović

    2005-09-01

    Full Text Available Postojeći informacioni sistem održavanja u Vojsci je, uz određene modifikacije, zadržan još iz perioda posle Drugog svetskog rata. Bilo je nekoliko pokušaja da se izvrši automatizacija, ali su svi pokušaji propali, bilo zbog pogrešnog pristupa ili pogrešnog izbora programskih alata. Reforme u Vojsci imaju značajan uticaj na organizaciju sistema održavanja, ali je uticaj na informacioni sistem održavanja neznatan. Prvi korak u automatizaciji predstavlja detaljna analiza procesa i tokova podataka čiju obradu treba automatizovati. Da bi automatizacija bila uspešna, analiza i deskripcija procesa i tokova podataka treba da bude rađena u nekom od suvremenih paketa za izradu informacionih sistema. Model informacionog sistema održavanja, opisan sa 19 potprocesa, nudi jedan od mogućih načina za automatizaciju, stoje i potvrđeno kroz program "Automatizovani informacioni sistem održavanja - trupni nivo". Ukoliko sistem odbrane naše zemlje teži međunarodnim integracijama, mora imati kompatibilne informacione sisteme, koji se mogu projektovati, kupiti ili ih mogu nametnuti međunarodne organizacije. / Available maintenance information system in the military forces is, with some modification, retained from the period after the World War П. There were several attempts of automatization of maintenance information systems, but all the attempts failed, whether because of the wrong approach or the wrong choice of program tools. A reform in military forces has got an important influence on organization of maintenance system, but the influence on the maintenance information system is minor. The first step in automatization is detailed analysis of processes and the dataflow that should be automated. For successful automatization, the analysis and process description ND dataflow should be made in some of the modern systems for information systems production. The model of maintenance information system, described with 19 sub-processes, offers

  20. Certification Systems

    Science.gov (United States)

    The WaterSense Product Certification System outlines the process and procedures for the product certification to ensure that all WaterSense labeled products meet EPA's criteria for efficiency and performance.