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Sample records for system effectors acting

  1. Effectors of Th1 and Th17 cells act on astrocytes and augment their neuroinflammatory properties.

    Science.gov (United States)

    Prajeeth, Chittappen K; Kronisch, Julius; Khorooshi, Reza; Knier, Benjamin; Toft-Hansen, Henrik; Gudi, Viktoria; Floess, Stefan; Huehn, Jochen; Owens, Trevor; Korn, Thomas; Stangel, Martin

    2017-10-16

    Autoreactive Th1 and Th17 cells are believed to mediate the pathology of multiple sclerosis in the central nervous system (CNS). Their interaction with microglia and astrocytes in the CNS is crucial for the regulation of the neuroinflammation. Previously, we have shown that only Th1 but not Th17 effectors activate microglia. However, it is not clear which cells are targets of Th17 effectors in the CNS. To understand the effects driven by Th17 cells in the CNS, we induced experimental autoimmune encephalomyelitis in wild-type mice and CD4 + T cell-specific integrin α4-deficient mice where trafficking of Th1 cells into the CNS was affected. We compared microglial and astrocyte response in the brain and spinal cord of these mice. We further treated astrocytes with supernatants from highly pure Th1 and Th17 cultures and assessed the messenger RNA expression of neurotrophic factors, cytokines and chemokines, using real-time PCR. Data obtained was analyzed using the Kruskal-Wallis test. We observed in α4-deficient mice weak microglial activation but comparable astrogliosis to that of wild-type mice in the regions of the brain populated with Th17 infiltrates, suggesting that Th17 cells target astrocytes and not microglia. In vitro, in response to supernatants from Th1 and Th17 cultures, astrocytes showed altered expression of neurotrophic factors, pro-inflammatory cytokines and chemokines. Furthermore, increased expression of chemokines in Th1- and Th17-treated astrocytes enhanced recruitment of microglia and transendothelial migration of Th17 cells in vitro. Our results demonstrate the delicate interaction between T cell subsets and glial cells and how they communicate to mediate their effects. Effectors of Th1 act on both microglia and astrocytes whereas Th17 effectors preferentially target astrocytes to promote neuroinflammation.

  2. System for exchanging tools and end effectors on a robot

    International Nuclear Information System (INIS)

    Burry, D.B.; Williams, P.M.

    1991-01-01

    A system and method for exchanging tools and end effectors on a robot permits exchange during a programmed task. The exchange mechanism is located off the robot, thus reducing the mass of the robot arm and permitting smaller robots to perform designated tasks. A simple spring/collet mechanism mounted on the robot is used which permits the engagement and disengagement of the tool or end effector without the need for a rotational orientation of the tool to the end effector/collet interface. As the tool changing system is not located on the robot arm no umbilical cords are located on robot. 12 figures

  3. Effectors of Th1 and Th17 cells act on astrocytes and augment their neuroinflammatory properties

    DEFF Research Database (Denmark)

    Prajeeth, Chittappen K; Kronisch, Julius; Khorooshi, Reza M. H.

    2017-01-01

    enhanced recruitment of microglia and transendothelial migration of Th17 cells in vitro. Conclusion: Our results demonstrate the delicate interaction between T cell subsets and glial cells and how they communicate to mediate their effects. Effectors of Th1 act on both microglia and astrocytes whereas Th17......Background: Autoreactive Th1 and Th17 cells are believed to mediate the pathology of multiple sclerosis in the central nervous system (CNS). Their interaction with microglia and astrocytes in the CNS is crucial for the regulation of the neuroinflammation. Previously we have shown that only Th1...... mice where trafficking of Th1 cells into the CNS was affected. We compared microglia and astrocyte response in the brain and spinal cord of these mice. We further treated astrocytes with supernatants from highly pure Th1 and Th17 cultures and assessed the mRNA expression of neurotrophic factors...

  4. Extra! Extracellular Effector Delivery into Host Cells via the Type 3 Secretion System

    OpenAIRE

    Kendall, Melissa M.

    2017-01-01

    ABSTRACT The type three secretion system (T3SS) is critical for the virulence of diverse bacterial pathogens. Pathogens use the T3SS to deliver effector proteins into host cells and manipulate host signaling pathways. The prevailing mechanism is that effectors translocate from inside the T3SS directly into the host cell. Recent studies reveal an alternative mechanism of effector translocation, in which an effector protein located outside the bacterial cell relies on the T3SS for delivery into...

  5. Type VI secretion system MIX-effectors carry both antibacterial and anti-eukaryotic activities.

    Science.gov (United States)

    Ray, Ann; Schwartz, Nika; de Souza Santos, Marcela; Zhang, Junmei; Orth, Kim; Salomon, Dor

    2017-11-01

    Most type VI secretion systems (T6SSs) described to date are protein delivery apparatuses that mediate bactericidal activities. Several T6SSs were also reported to mediate virulence activities, although only few anti-eukaryotic effectors have been described. Here, we identify three T6SSs in the marine bacterium Vibrio proteolyticus and show that T6SS1 mediates bactericidal activities under warm marine-like conditions. Using comparative proteomics, we find nine potential T6SS1 effectors, five of which belong to the polymorphic MIX-effector class. Remarkably, in addition to six predicted bactericidal effectors, the T6SS1 secretome includes three putative anti-eukaryotic effectors. One of these is a MIX-effector containing a cytotoxic necrotizing factor 1 domain. We demonstrate that T6SS1 can use this MIX-effector to target phagocytic cells, resulting in morphological changes and actin cytoskeleton rearrangements. In conclusion, the V. proteolyticus T6SS1, a system homologous to one found in pathogenic vibrios, uses a suite of polymorphic effectors that target both bacteria and eukaryotic neighbors. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.

  6. The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition.

    Science.gov (United States)

    Unterweger, Daniel; Miyata, Sarah T; Bachmann, Verena; Brooks, Teresa M; Mullins, Travis; Kostiuk, Benjamin; Provenzano, Daniele; Pukatzki, Stefan

    2014-04-01

    Vibrio cholerae is a Gram-negative bacterial pathogen that consists of over 200 serogroups with differing pathogenic potential. Only strains that express the virulence factors cholera toxin (CT) and toxin-coregulated pilus (TCP) are capable of pandemic spread of cholera diarrhoea. Regardless, all V. cholerae strains sequenced to date harbour genes for the type VI secretion system (T6SS) that translocates effectors into neighbouring eukaryotic and prokaryotic cells. Here we report that the effectors encoded within these conserved gene clusters differ widely among V. cholerae strains, and that immunity proteins encoded immediately downstream from the effector genes protect their host from neighbouring bacteria producing corresponding effectors. As a consequence, strains with matching effector-immunity gene sets can coexist, while strains with different sets compete against each other. Thus, the V. cholerae T6SS contributes to the competitive behaviour of this species.

  7. Type IV secretion system of Brucella spp. and its effectors.

    Science.gov (United States)

    Ke, Yuehua; Wang, Yufei; Li, Wengfeng; Chen, Zeliang

    2015-01-01

    Brucella spp. are intracellular bacterial pathogens that cause infection in domestic and wild animals. They are often used as model organisms to study intracellular bacterial infections. Brucella VirB T4SS is a key virulence factor that plays important roles in mediating intracellular survival and manipulating host immune response to infection. In this review, we discuss the roles of Brucella VirB T4SS and 15 effectors that are proposed to be crucial for Brucella pathogenesis. VirB T4SS regulates the inflammation response and manipulates vesicle trafficking inside host cells. VirB T4SS also plays crucial roles in the inhibition of the host immune response and intracellular survival during infection. Here, we list the key molecular events in the intracellular life cycle of Brucella that are potentially targeted by the VirB T4SS effectors. Elucidating the functions of these effectors will help clarify the molecular role of T4SS during infection. Furthermore, studying the effectors secreted by Brucella spp. might provide insights into the mechanisms used by the bacteria to hijack the host signaling pathways and aid in the development of better vaccines and therapies against brucellosis.

  8. System design description for the LDUA common video end effector system (CVEE)

    International Nuclear Information System (INIS)

    Pardini, A.F.

    1998-01-01

    The Common Video End Effector System (CVEE), system 62-60, was designed by the Idaho National Engineering Laboratory (INEL) to provide the control interface of the various video end effectors used on the LDUA. The CVEE system consists of a Support Chassis which contains the input and output Opto-22 modules, relays, and power supplies and the Power Chassis which contains the bipolar supply and other power supplies. The combination of the Support Chassis and the Power Chassis make up the CVEE system. The CVEE system is rack mounted in the At Tank Instrument Enclosure (ATIE). Once connected it is controlled using the LDUA supervisory data acquisition system (SDAS). Video and control status will be displayed on monitors within the LDUA control center

  9. Diversifying selection in the wheat stem rust fungus acts predominantly on pathogen-associated gene families and reveals candidate effectors.

    Science.gov (United States)

    Sperschneider, Jana; Ying, Hua; Dodds, Peter N; Gardiner, Donald M; Upadhyaya, Narayana M; Singh, Karam B; Manners, John M; Taylor, Jennifer M

    2014-01-01

    Plant pathogens cause severe losses to crop plants and threaten global food production. One striking example is the wheat stem rust fungus, Puccinia graminis f. sp. tritici, which can rapidly evolve new virulent pathotypes in response to resistant host lines. Like several other filamentous fungal and oomycete plant pathogens, its genome features expanded gene families that have been implicated in host-pathogen interactions, possibly encoding effector proteins that interact directly with target host defense proteins. Previous efforts to understand virulence largely relied on the prediction of secreted, small and cysteine-rich proteins as candidate effectors and thus delivered an overwhelming number of candidates. Here, we implement an alternative analysis strategy that uses the signal of adaptive evolution as a line of evidence for effector function, combined with comparative information and expression data. We demonstrate that in planta up-regulated genes that are rapidly evolving are found almost exclusively in pathogen-associated gene families, affirming the impact of host-pathogen co-evolution on genome structure and the adaptive diversification of specialized gene families. In particular, we predict 42 effector candidates that are conserved only across pathogens, induced during infection and rapidly evolving. One of our top candidates has recently been shown to induce genotype-specific hypersensitive cell death in wheat. This shows that comparative genomics incorporating the evolutionary signal of adaptation is powerful for predicting effector candidates for laboratory verification. Our system can be applied to a wide range of pathogens and will give insight into host-pathogen dynamics, ultimately leading to progress in strategies for disease control.

  10. Diversifying Selection in the Wheat Stem Rust Fungus Acts Predominantly on Pathogen-Associated Gene Families and Reveals Candidate Effectors

    Directory of Open Access Journals (Sweden)

    Jana eSperschneider

    2014-09-01

    Full Text Available Plant pathogens cause severe losses to crop plants and threaten global food production. One striking example is the wheat stem rust fungus, Puccinia graminis f. sp. tritici, which can rapidly evolve new virulent pathotypes in response to resistant host lines. Like several other filamentous fungal and oomycete plant pathogens, its genome features expanded gene families that have been implicated in host-pathogen interactions, possibly encoding effector proteins that interact directly with target host defence proteins. Previous efforts to understand virulence largely relied on the prediction of secreted, small and cysteine-rich proteins as candidate effectors and thus delivered an overwhelming number of candidates. Here, we implement an alternative analysis strategy that uses the signal of adaptive evolution as a line of evidence for effector function, combined with comparative information and expression data. We demonstrate that in planta up-regulated genes that are rapidly evolving are found almost exclusively in pathogen-associated gene families, affirming the impact of host-pathogen co-evolution on genome structure and the adaptive diversification of specialised gene families. In particular, we predict 42 effector candidates that are conserved only across pathogens, induced during infection and rapidly evolving. One of our top candidates has recently been shown to induce genotype-specific hypersensitive cell death in wheat. This shows that comparative genomics incorporating the evolutionary signal of adaptation is powerful for predicting effector candidates for laboratory verification. Our system can be applied to a wide range of pathogens and will give insight into host-pathogen dynamics, ultimately leading to progress in strategies for disease control.

  11. Design criteria for the light duty utility arm system end effectors

    International Nuclear Information System (INIS)

    Pardini, A.F.

    1995-01-01

    This document provides the criteria for the design of end effectors that will be used as part of the Light Duty Utility Arm (LDUA) System. The LDUA System consists of a deployment vehicle, a vertical positioning mast, a light duty multi-axis robotic arm, a tank riser interface and confinement, a tool interface plate, a control system, and an operations control trailer. The criteria specified in this document will apply to all end effector systems being developed for use on or with the LDUA system at the Hanford site. The requirement stipulated in this document are mandatory

  12. Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.

    Science.gov (United States)

    Vander Broek, Charles W; Chalmers, Kevin J; Stevens, Mark P; Stevens, Joanne M

    2015-04-01

    Burkholderia pseudomallei is an intracellular pathogen and the causative agent of melioidosis, a severe disease of humans and animals. One of the virulence factors critical for early stages of infection is the Burkholderia secretion apparatus (Bsa) Type 3 Secretion System (T3SS), a molecular syringe that injects bacterial proteins, called effectors, into eukaryotic cells where they subvert cellular functions to the benefit of the bacteria. Although the Bsa T3SS itself is known to be important for invasion, intracellular replication, and virulence, only a few genuine effector proteins have been identified and the complete repertoire of proteins secreted by the system has not yet been fully characterized. We constructed a mutant lacking bsaP, a homolog of the T3SS "gatekeeper" family of proteins that exert control over the timing and magnitude of effector protein secretion. Mutants lacking BsaP, or the T3SS translocon protein BipD, were observed to hypersecrete the known Bsa effector protein BopE, providing evidence of their role in post-translational control of the Bsa T3SS and representing key reagents for the identification of its secreted substrates. Isobaric Tags for Relative and Absolute Quantification (iTRAQ), a gel-free quantitative proteomics technique, was used to compare the secreted protein profiles of the Bsa T3SS hypersecreting mutants of B. pseudomallei with the isogenic parent strain and a bsaZ mutant incapable of effector protein secretion. Our study provides one of the most comprehensive core secretomes of B. pseudomallei described to date and identified 26 putative Bsa-dependent secreted proteins that may be considered candidate effectors. Two of these proteins, BprD and BapA, were validated as novel effector proteins secreted by the Bsa T3SS of B. pseudomallei. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  13. Imbalanced expression of functional surface molecules in regulatory and effector T cells in systemic lupus erythematosus

    International Nuclear Information System (INIS)

    Mesquita Júnior, D.; Cruvinel, W.M.; Araujo, J.A.P.; Salmazi, K.C.; Kallas, E.G.; Andrade, L.E.C.

    2014-01-01

    Regulatory T (TREG) cells play an important role in maintaining immune tolerance and avoiding autoimmunity. We analyzed the expression of membrane molecules in TREG and effector T cells in systemic lupus erythematosus (SLE). TREG and effector T cells were analyzed for the expression of CTLA-4, PD1, CD28, CD95, GITR, HLA-DR, OX40, CD40L, and CD45RO in 26 patients with active disease, 31 with inactive disease, and 26 healthy controls. TREG cells were defined as CD25 +/high CD127 Ø/low FoxP3 + , and effector T cells were defined as CD25 + CD127 + FoxP3 Ø . The ratio of TREG to effector T cells expressing GITR, PD1, HLA-DR, OX40, CD40L, and CD45RO was determined in the three groups. The frequency of TREG cells was similar in patients with SLE and controls. However, SLE patients had a decreased frequency of CTLA-4 + TREG and CD28 + TREG cells and an increased frequency of CD40L + TREG cells. There was a decrease in the TREG/effector-T ratio for GITR + , HLA-DR + , OX40 + , and CD45RO + cells, and an increased ratio of TREG/effector-T CD40L + cells in patients with SLE. In addition, CD40L + TREG cell frequency correlated with the SLE disease activity index (P=0.0163). In conclusion, our findings showed several abnormalities in the expression of functionally critical surface molecules in TREG and effector T cells in SLE that may be relevant to the pathogenesis of this disease

  14. Imbalanced expression of functional surface molecules in regulatory and effector T cells in systemic lupus erythematosus

    Energy Technology Data Exchange (ETDEWEB)

    Mesquita Júnior, D. [Disciplina de Reumatologia, Departamento de Medicina, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP (Brazil); Cruvinel, W.M. [Disciplina de Reumatologia, Departamento de Medicina, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP (Brazil); Departamento de Biomedicina, Universidade Católica de Goiás, Goiânia, GO (Brazil); Araujo, J.A.P. [Disciplina de Reumatologia, Departamento de Medicina, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP (Brazil); Salmazi, K.C.; Kallas, E.G. [Disciplina de Imunologia Clínica e Alergia, Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP (Brazil); Andrade, L.E.C. [Disciplina de Reumatologia, Departamento de Medicina, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP (Brazil)

    2014-08-22

    Regulatory T (TREG) cells play an important role in maintaining immune tolerance and avoiding autoimmunity. We analyzed the expression of membrane molecules in TREG and effector T cells in systemic lupus erythematosus (SLE). TREG and effector T cells were analyzed for the expression of CTLA-4, PD1, CD28, CD95, GITR, HLA-DR, OX40, CD40L, and CD45RO in 26 patients with active disease, 31 with inactive disease, and 26 healthy controls. TREG cells were defined as CD25{sup +/high}CD127{sup Ø/low}FoxP3{sup +}, and effector T cells were defined as CD25{sup +}CD127{sup +}FoxP3{sup Ø}. The ratio of TREG to effector T cells expressing GITR, PD1, HLA-DR, OX40, CD40L, and CD45RO was determined in the three groups. The frequency of TREG cells was similar in patients with SLE and controls. However, SLE patients had a decreased frequency of CTLA-4{sup +}TREG and CD28{sup +}TREG cells and an increased frequency of CD40L{sup +}TREG cells. There was a decrease in the TREG/effector-T ratio for GITR{sup +}, HLA-DR{sup +}, OX40{sup +}, and CD45RO{sup +} cells, and an increased ratio of TREG/effector-T CD40L{sup +} cells in patients with SLE. In addition, CD40L{sup +}TREG cell frequency correlated with the SLE disease activity index (P=0.0163). In conclusion, our findings showed several abnormalities in the expression of functionally critical surface molecules in TREG and effector T cells in SLE that may be relevant to the pathogenesis of this disease.

  15. Volumetric reach comparison of possible end-effectors for the articulated transporter and manipulator system

    Energy Technology Data Exchange (ETDEWEB)

    Kress, R.L.; Babcock, S.M.; Hamel, W.R. (Oak Ridge National Lab., TN (USA)); Bills, K.C. (Martin Marietta Energy Systems, Inc., Oak Ridge, TN (USA))

    1990-01-01

    The goal of this research was to investigate the performance of the Articulated Transporter and Manipulator System (ATMS) during various tasks relative to the choice of wrist/end-effector configuration. The approach taken was to generate computer graphics-aided three-dimensional interactive application (CATIA) system-based models of four wrist/end-effector combinations and consider the volumetric reach of each of these configurations based on the capacity of the ATMS. The results indicate that a simple, lightweight end-effector provides a greater volumetric reach. The greatest variation presented herein is {approximately}40% when comparing a 7-degree-of-freedom (DOF) dexterous arm with a simple 3-DOF arm; however, the benefit of increasing volumetric reach by only 40% by using a simple arm may be outweighed by the loss of dexterity. 10 refs., 5 figs., 3 tabs.

  16. A method for selecting cis-acting regulatory sequences that respond to small molecule effectors

    Directory of Open Access Journals (Sweden)

    Allas Ülar

    2010-08-01

    Full Text Available Abstract Background Several cis-acting regulatory sequences functioning at the level of mRNA or nascent peptide and specifically influencing transcription or translation have been described. These regulatory elements often respond to specific chemicals. Results We have developed a method that allows us to select cis-acting regulatory sequences that respond to diverse chemicals. The method is based on the β-lactamase gene containing a random sequence inserted into the beginning of the ORF. Several rounds of selection are used to isolate sequences that suppress β-lactamase expression in response to the compound under study. We have isolated sequences that respond to erythromycin, troleandomycin, chloramphenicol, meta-toluate and homoserine lactone. By introducing synonymous and non-synonymous mutations we have shown that at least in the case of erythromycin the sequences act at the peptide level. We have also tested the cross-activities of the constructs and found that in most cases the sequences respond most strongly to the compound on which they were isolated. Conclusions Several selected peptides showed ligand-specific changes in amino acid frequencies, but no consensus motif could be identified. This is consistent with previous observations on natural cis-acting peptides, showing that it is often impossible to demonstrate a consensus. Applying the currently developed method on a larger scale, by selecting and comparing an extended set of sequences, might allow the sequence rules underlying the activity of cis-acting regulatory peptides to be identified.

  17. Extra! Extracellular Effector Delivery into Host Cells via the Type 3 Secretion System.

    Science.gov (United States)

    Kendall, Melissa M

    2017-05-02

    The type three secretion system (T3SS) is critical for the virulence of diverse bacterial pathogens. Pathogens use the T3SS to deliver effector proteins into host cells and manipulate host signaling pathways. The prevailing mechanism is that effectors translocate from inside the T3SS directly into the host cell. Recent studies reveal an alternative mechanism of effector translocation, in which an effector protein located outside the bacterial cell relies on the T3SS for delivery into host cells. Tejeda-Dominguez et al. (F. Tejeda-Dominguez, J. Huerta-Cantillo, L. Chavez-Dueñas, and F. Navarro-Garcia, mBio 8:e00184-17, 2017, https://doi.org/10.1128/mBio.00184-17) demonstrate that the EspC effector of enteropathogenic Escherichia coli is translocated by binding to the outside of the T3SS and subsequently gains access to the host cell cytoplasm through the T3SS pore embedded within the host cell membrane. This work reveals a novel mechanism of translocation that is likely relevant for a variety of other pathogens that use the T3SS as part of their virulence arsenal. Copyright © 2017 Kendall.

  18. Structural basis of eukaryotic cell targeting by type III secretion system (T3SS) effectors.

    Science.gov (United States)

    Tosi, Tommaso; Pflug, Alexander; Discola, Karen F; Neves, David; Dessen, Andréa

    2013-01-01

    Type III secretion systems (T3SS) are macromolecular complexes that translocate a wide number of effector proteins into eukaryotic host cells. Once within the cytoplasm, many T3SS effectors mimic the structure and/or function of eukaryotic proteins in order to manipulate signaling cascades, and thus play pivotal roles in colonization, invasion, survival and virulence. Structural biology techniques have played key roles in the unraveling of bacterial strategies employed for mimicry and targeting. This review provides an overall view of our current understanding of structure and function of T3SS effectors, as well as of the different classes of eukaryotic proteins that are targeted and the consequences for the infected cell. Copyright © 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  19. Subversion of trafficking, apoptosis, and innate immunity by type III secretion system effectors.

    Science.gov (United States)

    Raymond, Benoit; Young, Joanna C; Pallett, Mitchell; Endres, Robert G; Clements, Abigail; Frankel, Gad

    2013-08-01

    Injection of effector proteins by a type III secretion system (T3SS) is a common infection strategy employed by many important human pathogens, including enteric Escherichia coli, Salmonella, Yersinia, and Shigella, to subvert cell signaling and host responses. In recent years, great advances have been made in understanding how the T3SS effectors function and execute the diverse infection strategies employed by these pathogens. In this review, we focus on effectors that subvert signaling pathways that impact on endosomal trafficking, cell survival, and innate immunity, particularly phagocytosis, nuclear factor-κB (NF-κB), and mitogen-activated protein (MAP) kinase pathways and the inflammasome. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. The Chlamydia type III secretion system C-ring engages a chaperone-effector protein complex.

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    Kris E Spaeth

    2009-09-01

    Full Text Available In Gram-negative bacterial pathogens, specialized chaperones bind to secreted effector proteins and maintain them in a partially unfolded form competent for translocation by type III secretion systems/injectisomes. How diverse sets of effector-chaperone complexes are recognized by injectisomes is unclear. Here we describe a new mechanism of effector-chaperone recognition by the Chlamydia injectisome, a unique and ancestral line of these evolutionarily conserved secretion systems. By yeast two-hybrid analysis we identified networks of Chlamydia-specific proteins that interacted with the basal structure of the injectisome, including two hubs of protein-protein interactions that linked known secreted effector proteins to CdsQ, the putative cytoplasmic C-ring component of the secretion apparatus. One of these protein-interaction hubs is defined by Ct260/Mcsc (Multiple cargo secretion chaperone. Mcsc binds to and stabilizes at least two secreted hydrophobic proteins, Cap1 and Ct618, that localize to the membrane of the pathogenic vacuole ("inclusion". The resulting complexes bind to CdsQ, suggesting that in Chlamydia, the C-ring of the injectisome mediates the recognition of a subset of inclusion membrane proteins in complex with their chaperone. The selective recognition of inclusion membrane proteins by chaperones may provide a mechanism to co-ordinate the translocation of subsets of inclusion membrane proteins at different stages in infection.

  1. Src Acts as an Effector for Ku70-dependent Suppression of Apoptosis through Phosphorylation of Ku70 at Tyr-530.

    Science.gov (United States)

    Morii, Mariko; Kubota, Sho; Honda, Takuya; Yuki, Ryuzaburo; Morinaga, Takao; Kuga, Takahisa; Tomonaga, Takeshi; Yamaguchi, Noritaka; Yamaguchi, Naoto

    2017-02-03

    Src-family tyrosine kinases are widely expressed in many cell types and participate in a variety of signal transduction pathways. Despite the significance of Src in suppression of apoptosis, its mechanism remains poorly understood. Here we show that Src acts as an effector for Ku70-dependent suppression of apoptosis. Inhibition of endogenous Src activity promotes UV-induced apoptosis, which is impaired by Ku70 knockdown. Src phosphorylates Ku70 at Tyr-530, being close to the possible acetylation sites involved in promotion of apoptosis. Src-mediated phosphorylation of Ku70 at Tyr-530 decreases acetylation of Ku70, whereas Src inhibition augments acetylation of Ku70. Importantly, knockdown-rescue experiments with stable Ku70 knockdown cells show that the nonphosphorylatable Y530F mutant of Ku70 reduces the ability of Ku70 to suppress apoptosis accompanied by augmentation of Ku70 acetylation. Our results reveal that Src plays a protective role against hyperactive apoptotic cell death by reducing apoptotic susceptibility through phosphorylation of Ku70 at Tyr-530. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  2. Computational approach to predict species-specific type III secretion system (T3SS) effectors using single and multiple genomes.

    Science.gov (United States)

    Hobbs, Christopher K; Porter, Vanessa L; Stow, Maxwell L S; Siame, Bupe A; Tsang, Herbert H; Leung, Ka Yin

    2016-12-19

    Many gram-negative bacteria use type III secretion systems (T3SSs) to translocate effector proteins into host cells. T3SS effectors can give some bacteria a competitive edge over others within the same environment and can help bacteria to invade the host cells and allow them to multiply rapidly within the host. Therefore, developing efficient methods to identify effectors scattered in bacterial genomes can lead to a better understanding of host-pathogen interactions and ultimately to important medical and biotechnological applications. We used 21 genomic and proteomic attributes to create a precise and reliable T3SS effector prediction method called Genome Search for Effectors Tool (GenSET). Five machine learning algorithms were trained on effectors selected from different organisms and a trained (voting) algorithm was then applied to identify other effectors present in the genome testing sets from the same (GenSET Phase 1) or different (GenSET Phase 2) organism. Although a select group of attributes that included the codon adaptation index, probability of expression in inclusion bodies, N-terminal disorder, and G + C content (filtered) were better at discriminating between positive and negative sets, algorithm performance was better when all 21 attributes (unfiltered) were used. Performance scores (sensitivity, specificity and area under the curve) from GenSET Phase 1 were better than those reported for six published methods. More importantly, GenSET Phase 1 ranked more known effectors (70.3%) in the top 40 ranked proteins and predicted 10-80% more effectors than three available programs in three of the four organisms tested. GenSET Phase 2 predicted 43.8% effectors in the top 40 ranked proteins when tested on four related or unrelated organisms. The lower prediction rates from GenSET Phase 2 may be due to the presence of different translocation signals in effectors from different T3SS families. The species-specific GenSET Phase 1 method offers an alternative

  3. Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality.

    Science.gov (United States)

    de Marco, Ario

    2017-01-01

    Molecular and chemical chaperones /foldases can strongly contribute to improve the amounts and the structural quality of recombinant proteins. Several methodologies have been proposed to optimize their beneficial effects. This chapter presents a condensed summary of the biotechnological opportunities offered by this approach followed by a protocol describing the method we use for expressing disulfide bond-dependent recombinant antibodies in the cytoplasm of bacteria engineered to overexpress sulfhydryl oxidase and DsbC isomerase. The system is based on the possibility to trigger the foldase expression independently and before the induction of the target protein. As a consequence, the recombinant antibody synthesis starts only after enough foldases have accumulated to promote correct folding of the antibody.

  4. NF1 Is an Effector and Regulator of the GPCR Signaling in the Nervous System

    Science.gov (United States)

    2015-04-01

    PRINCIPAL INVESTIGATOR: Kirill Martemyanov, Ph.D., Associate Professor CONTRACTING ORGANIZATION: The Scripps Research Institute – Florida Jupiter ...NF1 Is an Effector and Regulator of the GPCR Signaling in the Nervous System 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Kirill Martemyanov, Ph.D...ADDRESS(ES) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER The Scripps Research Institute – Florida 130 Scripps Way Jupiter , FL 33458-5284

  5. Mining the human gut microbiota for effector strains that shape the immune system.

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    Ahern, Philip P; Faith, Jeremiah J; Gordon, Jeffrey I

    2014-06-19

    The gut microbiota codevelops with the immune system beginning at birth. Mining the microbiota for bacterial strains responsible for shaping the structure and dynamic operations of the innate and adaptive arms of the immune system represents a formidable combinatorial problem but one that needs to be overcome to advance mechanistic understanding of microbial community and immune system coregulation and to develop new diagnostic and therapeutic approaches that promote health. Here, we discuss a scalable, less biased approach for identifying effector strains in complex microbial communities that impact immune function. The approach begins by identifying uncultured human fecal microbiota samples that transmit immune phenotypes to germ-free mice. Clonally arrayed sequenced collections of bacterial strains are constructed from representative donor microbiota. If the collection transmits phenotypes, effector strains are identified by testing randomly generated subsets with overlapping membership in individually housed germ-free animals. Detailed mechanistic studies of effector strain-host interactions can then be performed. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. SnTox3 acts in effector triggered susceptibility to induce disease on wheat carrying the Snn3 gene.

    Directory of Open Access Journals (Sweden)

    Zhaohui Liu

    2009-09-01

    Full Text Available The necrotrophic fungus Stagonospora nodorum produces multiple proteinaceous host-selective toxins (HSTs which act in effector triggered susceptibility. Here, we report the molecular cloning and functional characterization of the SnTox3-encoding gene, designated SnTox3, as well as the initial characterization of the SnTox3 protein. SnTox3 is a 693 bp intron-free gene with little obvious homology to other known genes. The predicted immature SnTox3 protein is 25.8 kDa in size. A 20 amino acid signal sequence as well as a possible pro sequence are predicted. Six cysteine residues are predicted to form disulfide bonds and are shown to be important for SnTox3 activity. Using heterologous expression in Pichia pastoris and transformation into an avirulent S. nodorum isolate, we show that SnTox3 encodes the SnTox3 protein and that SnTox3 interacts with the wheat susceptibility gene Snn3. In addition, the avirulent S. nodorum isolate transformed with SnTox3 was virulent on host lines expressing the Snn3 gene. SnTox3-disrupted mutants were deficient in the production of SnTox3 and avirulent on the Snn3 differential wheat line BG220. An analysis of genetic diversity revealed that SnTox3 is present in 60.1% of a worldwide collection of 923 isolates and occurs as eleven nucleotide haplotypes resulting in four amino acid haplotypes. The cloning of SnTox3 provides a fundamental tool for the investigation of the S. nodorum-wheat interaction, as well as vital information for the general characterization of necrotroph-plant interactions.

  7. Searching algorithm for type IV secretion system effectors 1.0: a tool for predicting type IV effectors and exploring their genomic context.

    Science.gov (United States)

    Meyer, Damien F; Noroy, Christophe; Moumène, Amal; Raffaele, Sylvain; Albina, Emmanuel; Vachiéry, Nathalie

    2013-11-01

    Type IV effectors (T4Es) are proteins produced by pathogenic bacteria to manipulate host cell gene expression and processes, divert the cell machinery for their own profit and circumvent the immune responses. T4Es have been characterized for some bacteria but many remain to be discovered. To help biologists identify putative T4Es from the complete genome of α- and γ-proteobacteria, we developed a Perl-based command line bioinformatics tool called S4TE (searching algorithm for type-IV secretion system effectors). The tool predicts and ranks T4E candidates by using a combination of 13 sequence characteristics, including homology to known effectors, homology to eukaryotic domains, presence of subcellular localization signals or secretion signals, etc. S4TE software is modular, and specific motif searches are run independently before ultimate combination of the outputs to generate a score and sort the strongest T4Es candidates. The user keeps the possibility to adjust various searching parameters such as the weight of each module, the selection threshold or the input databases. The algorithm also provides a GC% and local gene density analysis, which strengthen the selection of T4E candidates. S4TE is a unique predicting tool for T4Es, finding its utility upstream from experimental biology.

  8. Brassinosteriod Insensitive 2 (BIN2) acts as a downstream effector of the Target of Rapamycin (TOR) signaling pathway to regulate photoautotrophic growth in Arabidopsis.

    Science.gov (United States)

    Xiong, Fangjie; Zhang, Rui; Meng, Zhigang; Deng, Kexuan; Que, Yumei; Zhuo, Fengping; Feng, Li; Guo, Sundui; Datla, Raju; Ren, Maozhi

    2017-01-01

    The components of the target of rapamycin (TOR) signaling pathway have been well characterized in heterotrophic organisms from yeast to humans. However, because of rapamycin insensitivity, embryonic lethality in tor null mutants and a lack of reliable ways of detecting TOR protein kinase in higher plants, the key players upstream and downstream of TOR remain largely unknown in plants. Using engineered rapamycin-sensitive Binding Protein 12-2 (BP12-2) plants, the present study showed that combined treatment with rapamycin and active-site TOR inhibitors (asTORis) results in synergistic inhibition of TOR activity and plant growth in Arabidopsis. Based on this system, we revealed that TOR signaling plays a crucial role in modulating the transition from heterotrophic to photoautotrophic growth in Arabidopsis. Ribosomal protein S6 kinase 2 (S6K2) was identified as a direct downstream target of TOR, and the growth of TOR-suppressed plants could be rescued by up-regulating S6K2. Systems, genetic, and biochemical analyses revealed that Brassinosteriod Insensitive 2 (BIN2) acts as a novel downstream effector of S6K2, and the phosphorylation of BIN2 depends on TOR-S6K2 signaling in Arabidopsis. By combining pharmacological with genetic and biochemical approaches, we determined that the TOR-S6K2-BIN2 signaling pathway plays important roles in regulating the photoautotrophic growth of Arabidopsis. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  9. Regulators and effectors of bone morphogenetic protein signalling in the cardiovascular system.

    Science.gov (United States)

    Luo, Jiang-Yun; Zhang, Yang; Wang, Li; Huang, Yu

    2015-07-15

    Bone morphogenetic proteins (BMPs) play key roles in the regulation of cell proliferation, differentiation and apoptosis in various tissues and organs, including the cardiovascular system. BMPs signal through both Smad-dependent and -independent cascades to exert a wide spectrum of biological activities. Cardiovascular disorders such as abnormal angiogenesis, atherosclerosis, pulmonary hypertension and cardiac hypertrophy have been linked to aberrant BMP signalling. To correct the dysregulated BMP signalling in cardiovascular pathogenesis, it is essential to get a better understanding of how the regulators and effectors of BMP signalling control cardiovascular function and how the dysregulated BMP signalling contributes to cardiovascular dysfunction. We hence highlight several key regulators of BMP signalling such as extracellular regulators of ligands, mechanical forces, microRNAs and small molecule drugs as well as typical BMP effectors like direct downstream target genes, mitogen-activated protein kinases, reactive oxygen species and microRNAs. The insights into these molecular processes will help target both the regulators and important effectors to reverse BMP-associated cardiovascular pathogenesis. © 2015 The Authors. The Journal of Physiology © 2015 The Physiological Society.

  10. System properties, feedback control and effector coordination of human temperature regulation.

    Science.gov (United States)

    Werner, Jürgen

    2010-05-01

    The aim of human temperature regulation is to protect body processes by establishing a relative constancy of deep body temperature (regulated variable), in spite of external and internal influences on it. This is basically achieved by a distributed multi-sensor, multi-processor, multi-effector proportional feedback control system. The paper explains why proportional control implies inherent deviations of the regulated variable from the value in the thermoneutral zone. The concept of feedback of the thermal state of the body, conveniently represented by a high-weighted core temperature (T (c)) and low-weighted peripheral temperatures (T (s)) is equivalent to the control concept of "auxiliary feedback control", using a main (regulated) variable (T (c)), supported by an auxiliary variable (T (s)). This concept implies neither regulation of T (s) nor feedforward control. Steady-states result in the closed control-loop, when the open-loop properties of the (heat transfer) process are compatible with those of the thermoregulatory processors. They are called operating points or balance points and are achieved due to the inherent property of dynamical stability of the thermoregulatory feedback loop. No set-point and no comparison of signals (e.g. actual-set value) are necessary. Metabolic heat production and sweat production, though receiving the same information about the thermal state of the body, are independent effectors with different thresholds and gains. Coordination between one of these effectors and the vasomotor effector is achieved by the fact that changes in the (heat transfer) process evoked by vasomotor control are taken into account by the metabolic/sweat processor.

  11. Functionally redundant RXLR effectors from Phytophthora infestans act at different steps to suppress early flg22-triggered immunity.

    Directory of Open Access Journals (Sweden)

    Xiangzi Zheng

    2014-04-01

    Full Text Available Genome sequences of several economically important phytopathogenic oomycetes have revealed the presence of large families of so-called RXLR effectors. Functional screens have identified RXLR effector repertoires that either compromise or induce plant defense responses. However, limited information is available about the molecular mechanisms underlying the modes of action of these effectors in planta. The perception of highly conserved pathogen- or microbe-associated molecular patterns (PAMPs/MAMPs, such as flg22, triggers converging signaling pathways recruiting MAP kinase cascades and inducing transcriptional re-programming, yielding a generic anti-microbial response. We used a highly synchronizable, pathogen-free protoplast-based assay to identify a set of RXLR effectors from Phytophthora infestans (PiRXLRs, the causal agent of potato and tomato light blight that manipulate early stages of flg22-triggered signaling. Of thirty-three tested PiRXLR effector candidates, eight, called Suppressor of early Flg22-induced Immune response (SFI, significantly suppressed flg22-dependent activation of a reporter gene under control of a typical MAMP-inducible promoter (pFRK1-Luc in tomato protoplasts. We extended our analysis to Arabidopsis thaliana, a non-host plant species of P. infestans. From the aforementioned eight SFI effectors, three appeared to share similar functions in both Arabidopsis and tomato by suppressing transcriptional activation of flg22-induced marker genes downstream of post-translational MAP kinase activation. A further three effectors interfere with MAMP signaling at, or upstream of, the MAP kinase cascade in tomato, but not in Arabidopsis. Transient expression of the SFI effectors in Nicotiana benthamiana enhances susceptibility to P. infestans and, for the most potent effector, SFI1, nuclear localization is required for both suppression of MAMP signaling and virulence function. The present study provides a framework to decipher the

  12. Design criteria for the light duty utility arm system end effectors

    International Nuclear Information System (INIS)

    Pardini, A.F.; Kiebel, G.R.

    1995-12-01

    The purpose of this document is to provide criteria for the design of end effectors that will be used as part of the Light Duty Utility Arm (LDUA) System. Actual component design, fabrication, testing, and inspection will be performed by various DOE laboratories, industry, and academia. This document augments WHC-SD-TD-FRD-003, 'Functions and Requirements for the Light Duty Utility Arm Integrated System' (F). All requirements dictated in the F shall also be applicable in this document. Whenever conflicts arise between this document and the F, this document shall take precedence

  13. Physical and neural entrainment to rhythm: human sensorimotor coordination across tasks and effector systems

    Directory of Open Access Journals (Sweden)

    Jessica Marie Ross

    2014-08-01

    Full Text Available The human sensorimotor system can be readily entrained to environmental rhythms, through multiple sensory modalities. In this review, we provide an overview of theories of timekeeping that make this neuroentrainment possible. First, we present recent evidence that contests the assumptions made in classic timekeeper models. The role of state estimation, sensory feedback and movement parameters on the organization of sensorimotor timing are discussed in the context of recent experiments that examined simultaneous timing and force control. This discussion is extended to the study of coordinated multi-effector movements and how they may be entrained.

  14. Systems-Biology Approaches to Discover Anti-Viral Effectors of the Human Innate Immune Response

    Directory of Open Access Journals (Sweden)

    Andreas F.R. Sommer

    2011-07-01

    Full Text Available Virus infections elicit an immediate innate response involving antiviral factors. The activities of some of these factors are, in turn, blocked by viral countermeasures. The ensuing battle between the host and the viruses is crucial for determining whether the virus establishes a foothold and/or induces adaptive immune responses. A comprehensive systems-level understanding of the repertoire of anti-viral effectors in the context of these immediate virus-host responses would provide significant advantages in devising novel strategies to interfere with the initial establishment of infections. Recent efforts to identify cellular factors in a comprehensive and unbiased manner, using genome-wide siRNA screens and other systems biology “omics” methodologies, have revealed several potential anti-viral effectors for viruses like Human immunodeficiency virus type 1 (HIV-1, Hepatitis C virus (HCV, West Nile virus (WNV, and influenza virus. This review describes the discovery of novel viral restriction factors and discusses how the integration of different methods in systems biology can be used to more comprehensively identify the intimate interactions of viruses and the cellular innate resistance.

  15. Aeromonas salmonicida type III secretion system-effectors-mediated immune suppression in rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Origgi, F C; Benedicenti, O; Segner, H; Sattler, U; Wahli, T; Frey, J

    2017-01-01

    Aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. Together with other pathogens, it is characterized by the presence of a type 3 secretion system (T3SS). The T3SS is the main virulence mechanism of A. salmonicida. It is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. Some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributing to impair phagocytosis. Furthermore, it has been suggested that effectors of the T3SS are able to modulate the host's immune response. Here we present the first partial characterization of the immune response in rainbow trout (Oncorhynchus mykiss) infected with distinct strains of A. salmonicida either carrying (i) a fully functional T3SS or (ii) a functionally impaired T3SS or (iii) devoid of T3SS ("cured" strain). Infection with an A. salmonicida strain either carrying a fully functional or a secretion-impaired T3SS was associated with a strong and persistent immune suppression. However, the infection appeared to be fatal only in the presence of a fully functional T3SS. In contrast, the absence of T3SS was neither associated with immune suppression nor fish death. These findings suggest that the T3SS and T3SS-delivered effector molecules and toxins of A. salmonicida do not only impair the host cells' cytoskeleton thus damaging cell physiology and phagocytosis, but also heavily affect the transcription of critical immune mediators including the shut-down of important warning signals to recognize infection and induce immune defense. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  16. Genome-Wide Analysis of Type VI System Clusters and Effectors in Burkholderia Species

    Directory of Open Access Journals (Sweden)

    Thao Thi Nguyen

    2018-02-01

    Full Text Available Type VI secretion system (T6SS has been discovered in a variety of gram-negative bacteria as a versatile weapon to stimulate the killing of eukaryotic cells or prokaryotic competitors. Type VI secretion effectors (T6SEs are well known as key virulence factors for important pathogenic bacteria. In many Burkholderia species, T6SS has evolved as the most complicated secretion pathway with distinguished types to translocate diverse T6SEs, suggesting their essential roles in this genus. Here we attempted to detect and characterize T6SSs and potential T6SEs in target genomes of plant-associated and environmental Burkholderia species based on computational analyses. In total, 66 potential functional T6SS clusters were found in 30 target Burkholderia bacterial genomes, of which 33% possess three or four clusters. The core proteins in each cluster were specified and phylogenetic trees of three components (i.e., TssC, TssD, TssL were constructed to elucidate the relationship among the identified T6SS clusters. Next, we identified 322 potential T6SEs in the target genomes based on homology searches and explored the important domains conserved in effector candidates. In addition, using the screening approach based on the profile hidden Markov model (pHMM of T6SEs that possess markers for type VI effectors (MIX motif (MIX T6SEs, 57 revealed proteins that were not included in training datasets were recognized as novel MIX T6SE candidates from the Burkholderia species. This approach could be useful to identify potential T6SEs from other bacterial genomes.

  17. Diverse toxic effectors are harbored by vgrG islands for interbacterial antagonism in type VI secretion system.

    Science.gov (United States)

    Ma, Jiale; Sun, Min; Pan, Zihao; Lu, Chengping; Yao, Huochun

    2018-04-16

    The type VI secretion system (T6SS) is considered as one of the key competition strategies by injecting toxic effectors for intestinal pathogens to acquire optimal colonization in host gut, a microenviroment with high-density polymicrobial community where bacteria compete for niches and resources. Enterotoxigenic Escherichia coli (ETEC), a major cause of infectious diarrhea in human and animals, widely encode T6SS clusters in their genomes. In this report, we first identified VT1, a novel amidase effector in ETEC, significantly hydrolyzed D-lactyl-L-Ala crosslinks between N-acetylmuramoyl and L-Ala in peptidoglycan. Further study showed that the VT1/VTI1 effector/immunity pair is encoded within a typical vgrG island, and plays a critical role for the successful establishment of ETEC in host gut. Numerous putative effectors with diverse toxin domains were found by retrieving vgrG islands in pathogenic E. coli, and designated as VT modules. Therein, VT5, a lysozyme-like effector widely encoded in ETEC, was confirmed to effectively kill adjacent cells, suggesting that VT toxin modules may be critical for pathogenic E. coli to seize a significantly competitive advantage for optimal intestinal colonization. To expand our analyses for large-scale search of VT antibacterial effectors based on vgrG island, >200 predicted effectors from 20 bacterial species were found and classified into 11 predicted toxins. This work reports a new retrieval strategy for screening T6SS effectors, and provides an example how pathogenic bacteria antagonize and displace commensal microbiome to successfully colonize in the host niches through a T6SS-dependent manner. Copyright © 2018. Published by Elsevier B.V.

  18. Diversifying selection in the wheat stem rust fungus acts predominantly on pathogen-associated gene families and reveals candidate effectors

    OpenAIRE

    Sperschneider, Jana; Ying, Hua; Dodds, Peter N.; Gardiner, Donald M.; Upadhyaya, Narayana M.; Singh, Karam B.; Manners, John M.; Taylor, Jennifer M.

    2014-01-01

    Plant pathogens cause severe losses to crop plants and threaten global food production. One striking example is the wheat stem rust fungus, Puccinia graminis f. sp. tritici, which can rapidly evolve new virulent pathotypes in response to resistant host lines. Like several other filamentous fungal and oomycete plant pathogens, its genome features expanded gene families that have been implicated in host-pathogen interactions, possibly encoding effector proteins that interact directly with targe...

  19. Bacterial Effectors and Their Functions in the Ubiquitin-Proteasome System: Insight from the Modes of Substrate Recognition

    Directory of Open Access Journals (Sweden)

    Minsoo Kim

    2014-08-01

    Full Text Available Protein ubiquitination plays indispensable roles in the regulation of cell homeostasis and pathogenesis of neoplastic, infectious, and neurodegenerative diseases. Given the importance of this modification, it is to be expected that several pathogenic bacteria have developed the ability to utilize the host ubiquitin system for their own benefit. Modulation of the host ubiquitin system by bacterial effector proteins inhibits innate immune responses and hijacks central signaling pathways. Bacterial effectors mimic enzymes of the host ubiquitin system, but may or may not be structurally similar to the mammalian enzymes. Other effectors bind and modify components of the host ubiquitin system, and some are themselves subject to ubiquitination. This review will describe recent findings, based on structural analyses, regarding how pathogens use post-translational modifications of proteins to establish an infection.

  20. Bacterial effectors and their functions in the ubiquitin-proteasome system: insight from the modes of substrate recognition.

    Science.gov (United States)

    Kim, Minsoo; Otsubo, Ryota; Morikawa, Hanako; Nishide, Akira; Takagi, Kenji; Sasakawa, Chihiro; Mizushima, Tsunehiro

    2014-08-18

    Protein ubiquitination plays indispensable roles in the regulation of cell homeostasis and pathogenesis of neoplastic, infectious, and neurodegenerative diseases. Given the importance of this modification, it is to be expected that several pathogenic bacteria have developed the ability to utilize the host ubiquitin system for their own benefit. Modulation of the host ubiquitin system by bacterial effector proteins inhibits innate immune responses and hijacks central signaling pathways. Bacterial effectors mimic enzymes of the host ubiquitin system, but may or may not be structurally similar to the mammalian enzymes. Other effectors bind and modify components of the host ubiquitin system, and some are themselves subject to ubiquitination. This review will describe recent findings, based on structural analyses, regarding how pathogens use post-translational modifications of proteins to establish an infection.

  1. Kinematics Analysis of End Effector for Carrier Robot of Feeding Broiler Chicken System

    Science.gov (United States)

    Syam, Rafiuddin; Arsyad, Hairul; Bauna, Ruslan; Renreng, Ilyas; Bakhri, Syaiful

    2018-02-01

    The demand for commodities, especially Broiler chicken farms are increasing, the volume of feed requirements Broiler chickens increased with age up to the age of 30-57 days required feed 3,829 grams/day/head, so if the chicken population is 3,000 needed transporting feed 11 487 kg/day, This research aims to produce a robot capable of transporting feed in the top of the cage by using a control system so as to make efficient use of manpower. Design robot performed using software design three-dimensional Solidworks2010, process of making the robot is started with the design manufacture three (3) units of mechanical systems (mechanical system for holder feed, mechanical systems for lifter feed and mechanical systems for transporting feed), then do the design process framework as a component buffer so that the mechanical system will work properly and safely when the robot operates. Furthermore, the manufacture of electronic circuits and control are using Arduino Mega microcontroller. After assembling all components mechanical systems and installation of electronic systems and control, then experiments to evaluate the performance of the robot have been made. The results of experiments showed that all components work well according to plan, in particular the speed and acceleration of end effector motion so it can hold and release the feed well. This strongly supports the robots perform tasks in accordance with the intent, i.e., holding, lifting and moving feed.

  2. Implications of Spatiotemporal Regulation of Shigella flexneri Type Three Secretion Activity on Effector Functions: Think Globally, Act Locally

    Science.gov (United States)

    Campbell-Valois, F.-X.; Pontier, Stéphanie M.

    2016-01-01

    Shigella spp. are Gram-negative bacterial pathogens that infect human colonic epithelia and cause bacterial dysentery. These bacteria express multiple copies of a syringe-like protein complex, the Type Three Secretion apparatus (T3SA), which is instrumental in the etiology of the disease. The T3SA triggers the plasma membrane (PM) engulfment of the bacteria by host cells during the initial entry process. It then enables bacteria to escape the resulting phagocytic-like vacuole. Freed bacteria form actin comets to move in the cytoplasm, which provokes bacterial collision with the inner leaflet of the PM. This phenomenon culminates in T3SA-dependent secondary uptake and vacuolar rupture in neighboring cells in a process akin to what is observed during entry and named cell-to-cell spread. The activity of the T3SA of Shigella flexneri was recently demonstrated to display an on/off regulation during the infection. While the T3SA is active when bacteria are in contact with PM-derived compartments, it switches to an inactive state when bacteria are released within the cytosol. These observations indicate that effector proteins transiting through the T3SA are therefore translocated in a highly time and space constrained fashion, likely impacting on their cellular distribution. Herein, we present what is currently known about the composition, the assembly and the regulation of the T3SA activity and discuss the consequences of the on/off regulation of T3SA on Shigella effector properties and functions during the infection. Specific examples that will be developed include the role of effectors IcsB and VirA in the escape from LC3/ATG8-positive vacuoles formed during cell-to-cell spread and of IpaJ protease activity against N-miristoylated proteins. The conservation of a similar regulation of T3SA activity in other pathogens such as Salmonella or Enteropathogenic Escherichia coli will also be briefly discussed. PMID:27014638

  3. Implications of Spatiotemporal Regulation of Shigella flexneri Type three Secretion Activity on Effector Functions: think globally, act locally

    Directory of Open Access Journals (Sweden)

    F-X eCampbell-Valois

    2016-03-01

    Full Text Available Shigella spp. are Gram-negative bacterial pathogens that infect human colonic epithelia and cause bacterial dysentery. These bacteria express multiple copies of a syringe-like protein complex, the Type Three Secretion apparatus (T3SA, which is instrumental in the etiology of the disease. The T3SA triggers the plasma membrane (PM engulfment of the bacteria by host cells during the initial entry process. It then enables bacteria to escape the resulting phagocytic-like vacuole. Freed bacteria form actin comets to move in the cytoplasm, which provokes bacterial collision with the inner leaflet of the PM. This phenomenon culminates in T3SA-dependent secondary uptake and vacuolar rupture in neighboring cells in a process akin to what is observed during entry and named cell-to-cell spread. The activity of the T3SA of Shigella flexneri was recently demonstrated to display an on/off regulation during the infection. While the T3SA is active when bacteria are in contact with PM-derived compartments, it switches to an inactive state when bacteria are released within the cytosol. These observations indicate that effector proteins transiting through the T3SA are therefore translocated in a highly time and space constrained fashion, likely impacting on their cellular distribution. Herein, we present what is currently known about the composition, the assembly and the regulation of the T3SA activity and discuss the consequences of the on/off regulation of T3SA on Shigella effector properties and functions during the infection. Specific examples that will be developed include the role of effectors IcsB and VirA in the escape from LC3/ATG8-positive vacuoles formed during cell-to-cell spread and of IpaJ protease activity against N-miristoylated proteins. The conservation of a similar regulation of T3SA activity in other pathogens such as Salmonella or Enteropathogenic Escherichia coli will also be briefly discussed.

  4. Exploitation of eukaryotic ubiquitin signaling pathways by effectors translocated by bacterial type III and type IV secretion systems.

    Directory of Open Access Journals (Sweden)

    Aurélie Angot

    2007-01-01

    Full Text Available The specific and covalent addition of ubiquitin to proteins, known as ubiquitination, is a eukaryotic-specific modification central to many cellular processes, such as cell cycle progression, transcriptional regulation, and hormone signaling. Polyubiquitination is a signal for the 26S proteasome to destroy earmarked proteins, but depending on the polyubiquitin chain topology, it can also result in new protein properties. Both ubiquitin-orchestrated protein degradation and modification have also been shown to be essential for the host's immune response to pathogens. Many animal and plant pathogenic bacteria utilize type III and/or type IV secretion systems to inject effector proteins into host cells, where they subvert host signaling cascades as part of their infection strategy. Recent progress in the determination of effector function has taught us that playing with the host's ubiquitination system seems a general tactic among bacteria. Here, we discuss how bacteria exploit this system to control the timing of their effectors' action by programming them for degradation, to block specific intermediates in mammalian or plant innate immunity, or to target host proteins for degradation by mimicking specific ubiquitin/proteasome system components. In addition to analyzing the effectors that have been described in the literature, we screened publicly available bacterial genomes for mimicry of ubiquitin proteasome system subunits and detected several new putative effectors. Our understanding of the intimate interplay between pathogens and their host's ubiquitin proteasome system is just beginning. This exciting research field will aid in better understanding this interplay, and may also provide new insights into eukaryotic ubiquitination processes.

  5. Effector protein translocation by the Coxiella burnetii Dot/Icm type IV secretion system requires endocytic maturation of the pathogen-occupied vacuole.

    Directory of Open Access Journals (Sweden)

    Hayley J Newton

    Full Text Available The human pathogen Coxiella burnetii encodes a type IV secretion system called Dot/Icm that is essential for intracellular replication. The Dot/Icm system delivers bacterial effector proteins into the host cytosol during infection. The effector proteins delivered by C. burnetii are predicted to have important functions during infection, but when these proteins are needed during infection has not been clearly defined. Here, we use a reporter system consisting of fusion proteins that have a β-lactamase enzyme (BlaM fused to C. burnetii effector proteins to study protein translocation by the Dot/Icm system. Translocation of BlaM fused to the effector proteins CBU0077, CBU1823 and CBU1524 was not detected until 8-hours after infection of HeLa cells, which are permissive for C. burnetii replication. Translocation of these effector fusion proteins by the Dot/Icm system required acidification of the Coxiella-containing vacuole. Silencing of the host genes encoding the membrane transport regulators Rab5 or Rab7 interfered with effector translocation, which indicates that effectors are not translocated until bacteria traffic to a late endocytic compartment in the host cell. Similar requirements for effector translocation were discerned in bone marrow macrophages derived from C57BL/6 mice, which are primary cells that restrict the intracellular replication of C. burnetii. In addition to requiring endocytic maturation of the vacuole for Dot/Icm-mediated translocation of effectors, bacterial transcription was required for this process. Thus, translocation of effector proteins by the C. burnetii Dot/Icm system occurs after acidification of the CCV and maturation of this specialized organelle to a late endocytic compartment. This indicates that creation of the specialized vacuole in which C. burnetii replicates represents a two-stage process mediated initially by host factors that regulate endocytic maturation and then by bacterial effectors delivered into

  6. A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors

    Directory of Open Access Journals (Sweden)

    Uhde-Stone Claudia

    2012-08-01

    Full Text Available Abstract Background Transcription activator-like effectors (TALEs are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence-specific perturbation and offers broad applications in genetic and epigenetic studies. Although the efficient construction of TALEs has been established, robust functional tools to assess their functions remain lacking. Results We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs. We validated both sensitivity and specificity of this dual-reporter system in mammalian cells, and demonstrated that this dual reporter system is robust and potentially amenable to high throughput (HTP applications. Conclusion We have designed, constructed and validated a novel dual reporter system for assessing TALE mediated gene regulations. This system offers a robust and easy-to- use tool for real-time monitoring and quantifying gene expression in mammalian cells.

  7. New players in the same old game: a system level in silico study to predict type III secretion system and effector proteins in bacterial genomes reveals common themes in T3SS mediated pathogenesis.

    Science.gov (United States)

    Sadarangani, Vineet; Datta, Sunando; Arunachalam, Manonmani

    2013-07-26

    Type III secretion system (T3SS) plays an important role in virulence or symbiosis of many pathogenic or symbiotic bacteria [CHM 2:291-294, 2007; Physiology (Bethesda) 20:326-339, 2005]. T3SS acts like a tunnel between a bacterium and its host through which the bacterium injects 'effector' proteins into the latter [Nature 444:567-573, 2006; COSB 18:258-266, 2008]. The effectors spatially and temporally modify the host signalling pathways [FEMS Microbiol Rev 35:1100-1125, 2011; Cell Host Microbe5:571-579, 2009]. In spite its crucial role in host-pathogen interaction, the study of T3SS and the associated effectors has been limited to a few bacteria [Cell Microbiol 13:1858-1869, 2011; Nat Rev Microbiol 6:11-16, 2008; Mol Microbiol 80:1420-1438, 2011]. Before one set out to perform systematic experimental studies on an unknown set of bacteria it would be beneficial to identify the potential candidates by developing an in silico screening algorithm. A system level study would also be advantageous over traditional laboratory methods to extract an overriding theme for host-pathogen interaction, if any, from the vast resources of data generated by sequencing multiple bacterial genomes. We have developed an in silico protocol in which the most conserved set of T3SS proteins was used as the query against the entire bacterial database with increasingly stringent search parameters. It enabled us to identify several uncharacterized T3SS positive bacteria. We adopted a similar strategy to predict the presence of the already known effectors in the newly identified T3SS positive bacteria. The huge resources of biochemical data [FEMS Microbiol Rev 35:1100-1125, 2011; Cell Host Microbe 5:571-579, 2009; BMC Bioinformatics 7(11):S4, 2010] on the T3SS effectors enabled us to search for the common theme in T3SS mediated pathogenesis. We identified few cellular signalling networks in the host, which are manipulated by most of the T3SS containing pathogens. We went on to look for

  8. Effector T-cells are expanded in systemic lupus erythematosus patients with high disease activity and damage indexes.

    Science.gov (United States)

    Piantoni, S; Regola, F; Zanola, A; Andreoli, L; Dall'Ara, F; Tincani, A; Airo', P

    2018-01-01

    Background and objectives T-cell activation may be one of the pathogenic mechanisms of systemic lupus erythematosus (SLE). After repeated antigenic stimulation, T-cells undergo different modifications, leading to the differentiation into effector memory T-cells (CCR7-CD45RA-) and terminally differentiated effector memory (TDEM) T-cells (CCR7-CD45RA+). Similarly, down-modulation of CD28 may lead to the expansion of the CD28- T-cells, a subpopulation with peculiar effector activities. The aim of this study was the characterization of T-cell phenotype in a cohort of patients with SLE according to disease activity and damage index. Materials and methods Phenotypic analysis of peripheral blood T lymphocytes of 51 SLE patients and 21 healthy controls was done by flow-cytometry. SLE disease activity was evaluated by SLE Disease Activity Index-2000 (SLEDAI-2K) and damage by the Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index (SDI). The variations between different groups were evaluated by Mann-Whitney test. Bonferroni correction was applied to adjust for multiple comparisons ( p adj ). Spearman rank test was used to evaluate the correlations between quantitative variables. Results CD4+ lymphopenia was found among SLE patients. Patients showed a trend for a higher percentage of TDEM among the CD4+ T-cell subpopulation in comparison with healthy controls ( p = .04). SLE patients were divided into two groups according to disease activity: patients with SLEDAI-2K ≥ 6 ( n = 13) had a higher percentage of circulating CD4+ T-cells with CD28- phenotype ( p adj  = .005) as well as those with an effector memory ( p adj  = .004) and TDEM ( p adj  = .002) phenotype and a trend of decrease of regulatory T-cells (TREGs) ( p = .02), in comparison with patients with low disease activity ( n = 38). Patients with damage (SDI ≥ 1) tended to show an expansion of TDEM among CD4+ T-cells as compared with

  9. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.

    Science.gov (United States)

    Frencher, James T; Shen, Hongbo; Yan, Lin; Wilson, Jessica O; Freitag, Nancy E; Rizzo, Alicia N; Chen, Crystal Y; Chen, Zheng W

    2014-12-01

    Whereas infection or immunization of humans/primates with microbes coproducing HMBPP/IPP can remarkably activate Vγ2Vδ2 T cells, in vivo studies have not been done to dissect HMBPP- and IPP-driven expansion, pulmonary trafficking, effector functions, and memory polarization of Vγ2Vδ2 T cells. We define these phosphoantigen-host interplays by comparative immunizations of macaques with the HMBPP/IPP-coproducing Listeria ΔactA prfA* and HMBPP-deficient Listeria ΔactA ΔGCPE: prfA* mutant. The HMBPP-deficient ΔGCPE: mutant shows lower ability to expand Vγ2Vδ2 T cells in vitro than the parental HMBPP-producing strain but displays comparably attenuated infectivity or immunogenicity. Respiratory immunization of macaques with the HMBPP-deficient mutant elicits lower pulmonary and systemic responses of Vγ2Vδ2 T cells compared with the HMBPP-producing vaccine strain. Interestingly, HMBPP-deficient mutant reimmunization or boosting elicits enhanced responses of Vγ2Vδ2 T cells, but the magnitude is lower than that by HMBPP-producing listeria. HMBPP-deficient listeria differentiated fewer Vγ2Vδ2 T effector cells capable of coproducing IFN-γ and TNF-α and inhibiting intracellular listeria than HMBPP-producing listeria. Furthermore, HMBPP deficiency in listerial immunization influences memory polarization of Vγ2Vδ2 T cells. Thus, both HMBPP and IPP production in listerial immunization or infection elicit systemic/pulmonary responses and differentiation of Vγ2Vδ2 T cells, but a role for HMBPP is more dominant. Findings may help devise immune intervention. © 2014 Society for Leukocyte Biology.

  10. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells

    Science.gov (United States)

    Frencher, James T.; Shen, Hongbo; Yan, Lin; Wilson, Jessica O.; Freitag, Nancy E.; Rizzo, Alicia N.; Chen, Crystal Y.; Chen, Zheng W.

    2014-01-01

    Whereas infection or immunization of humans/primates with microbes coproducing HMBPP/IPP can remarkably activate Vγ2Vδ2 T cells, in vivo studies have not been done to dissect HMBPP- and IPP-driven expansion, pulmonary trafficking, effector functions, and memory polarization of Vγ2Vδ2 T cells. We define these phosphoantigen-host interplays by comparative immunizations of macaques with the HMBPP/IPP-coproducing Listeria ΔactA prfA* and HMBPP-deficient Listeria ΔactAΔgcpE prfA* mutant. The HMBPP-deficient ΔgcpE mutant shows lower ability to expand Vγ2Vδ2 T cells in vitro than the parental HMBPP-producing strain but displays comparably attenuated infectivity or immunogenicity. Respiratory immunization of macaques with the HMBPP-deficient mutant elicits lower pulmonary and systemic responses of Vγ2Vδ2 T cells compared with the HMBPP-producing vaccine strain. Interestingly, HMBPP-deficient mutant reimmunization or boosting elicits enhanced responses of Vγ2Vδ2 T cells, but the magnitude is lower than that by HMBPP-producing listeria. HMBPP-deficient listeria differentiated fewer Vγ2Vδ2 T effector cells capable of coproducing IFN-γ and TNF-α and inhibiting intracellular listeria than HMBPP-producing listeria. Furthermore, HMBPP deficiency in listerial immunization influences memory polarization of Vγ2Vδ2 T cells. Thus, both HMBPP and IPP production in listerial immunization or infection elicit systemic/pulmonary responses and differentiation of Vγ2Vδ2 T cells, but a role for HMBPP is more dominant. Findings may help devise immune intervention. PMID:25114162

  11. Effector Mechanisms of Neutrophils within the Innate Immune System in Response to Mycobacterium tuberculosis Infection.

    Science.gov (United States)

    Warren, Eric; Teskey, Garrett; Venketaraman, Vishwanath

    2017-02-07

    Neutrophils have a significant yet controversial role in the innate immune response to Mycobacterium tuberculosis ( M. tb ) infection, which is not yet fully understood. In addition to neutrophils' well-known effector mechanisms, they may also help control infection of M. tb through the formation of neutrophil extracellular traps (NETs), which are thought to further promote the killing of M. tb by resident alveolar macrophages. Cytokines such as IFN-γ have now been shown to serve an immunomodulatory role in neutrophil functioning in conjunction to its pro-inflammatory function. Additionally, the unique transcriptional changes of neutrophils may be used to differentiate between infection with M. tb and other bacterial and chronic rheumatological diseases such as Systemic Lupus Erythematosus. Adversely, during the innate immune response to M. tb , inappropriate phagocytosis of spent neutrophils can result in nonspecific damage to host cells due to necrotic lysis. Furthermore, some individuals have been shown to be more genetically susceptible to tuberculosis (TB) due to a "Trojan Horse" phenomenon whereby neutrophils block the ability of resident macrophages to kill M. tb . Despite these aforementioned negative consequences, through the scope of this review we will provide evidence to support the idea that neutrophils, while sometimes damaging, can also be an important component in warding off M. tb infection. This is exemplified in immunocompromised individuals, such as those with human immunodeficiency virus (HIV) infection or Type 2 diabetes mellitus. These individuals are at an increased risk of developing tuberculosis (TB) due to a diminished innate immune response associated with decreased levels of glutathione. Consequently, there has been a worldwide effort to limit and contain M. tb infection through the use of antibiotics and vaccinations. However, due to several significant limitations, the current bacille Calmette-Guerin vaccine (BCG, vaccine against TB

  12. Effector Mechanisms of Neutrophils within the Innate Immune System in Response to Mycobacterium tuberculosis Infection

    Directory of Open Access Journals (Sweden)

    Eric Warren

    2017-02-01

    Full Text Available Neutrophils have a significant yet controversial role in the innate immune response to Mycobacterium tuberculosis (M. tb infection, which is not yet fully understood. In addition to neutrophils’ well-known effector mechanisms, they may also help control infection of M. tb through the formation of neutrophil extracellular traps (NETs, which are thought to further promote the killing of M. tb by resident alveolar macrophages. Cytokines such as IFN-γ have now been shown to serve an immunomodulatory role in neutrophil functioning in conjunction to its pro-inflammatory function. Additionally, the unique transcriptional changes of neutrophils may be used to differentiate between infection with M. tb and other bacterial and chronic rheumatological diseases such as Systemic Lupus Erythematosus. Adversely, during the innate immune response to M. tb, inappropriate phagocytosis of spent neutrophils can result in nonspecific damage to host cells due to necrotic lysis. Furthermore, some individuals have been shown to be more genetically susceptible to tuberculosis (TB due to a “Trojan Horse” phenomenon whereby neutrophils block the ability of resident macrophages to kill M. tb. Despite these aforementioned negative consequences, through the scope of this review we will provide evidence to support the idea that neutrophils, while sometimes damaging, can also be an important component in warding off M. tb infection. This is exemplified in immunocompromised individuals, such as those with human immunodeficiency virus (HIV infection or Type 2 diabetes mellitus. These individuals are at an increased risk of developing tuberculosis (TB due to a diminished innate immune response associated with decreased levels of glutathione. Consequently, there has been a worldwide effort to limit and contain M. tb infection through the use of antibiotics and vaccinations. However, due to several significant limitations, the current bacille Calmette-Guerin vaccine (BCG

  13. Agency Correspondence Tracking System (ACTS)

    Data.gov (United States)

    US Agency for International Development — Agency Correspondence Tracking System: is an executive correspondence tracking system for the Administrator. It collects and organizes information on reports...

  14. A translocator-specific export signal establishes the translocator-effector secretion hierarchy that is important for type III secretion system function

    Science.gov (United States)

    Tomalka, Amanda G.; Stopford, Charles M.; Lee, Pei-Chung; Rietsch, Arne

    2012-01-01

    Summary Type III secretion systems are used by many Gram-negative pathogens to directly deliver effector proteins into the cytoplasm of host cells. To accomplish this, bacteria secrete translocator proteins that form a pore in the host-cell membrane through which the effector proteins are then introduced into the host cell. Evidence from multiple systems indicates that the pore-forming translocator proteins are exported before effectors, but how this secretion hierarchy is established is unclear. Here we used the P. aeruginosa translocator protein PopD as a model to identify its export signals. The amino-terminal secretion signal and chaperone, PcrH, are required for export under all conditions. Two novel signals in PopD, one proximal to the chaperone-binding site and one at the very C-terminus of the protein, are required for export of PopD before effector proteins. These novel export signals establish the translocator-effector secretion hierarchy, which in turn, is critical for the delivery of effectors into host cells. PMID:23121689

  15. Freedom of Information Act (FOIA) Tracking System

    Data.gov (United States)

    Office of Personnel Management — Tracking system which allows for the input of the Freedom of Information Act (FOIA) request date, compiles the due date, information pertaining to the request, name...

  16. 76 FR 64115 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2011-10-17

    ... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (11-092)] Privacy Act of 1974; Privacy Act... retirement of one Privacy Act system of records notice. SUMMARY: In accordance with the Privacy Act of 1974, NASA is giving notice that it proposes to cancel the following Privacy Act system of records notice...

  17. Suppressive versus augmenting effect of the same pretreatment regimen in two murine tumor systems with distinct effector mechanisms

    International Nuclear Information System (INIS)

    Fujiwara, Hiromi; Hamaoka, Toshiyuki; Kitagawa, Masayasu

    1978-01-01

    The effect of presensitization with x-irradiated tumor cells on the development of host's immune resistance against the tumor-associated transplantation antigens (TATA) was investigated in two syngeneic tumor systems with distinct effector mechanisms. When X5563 plasmacytoma, to which immune resistance was mediated exclusively by killer T lymphocytes, was intravenously inoculated into syngeneic C3H/He mice with lower number after 7000 R x-irradiation, the mice failed to exhibit any protective immunity against the subsequent challenge with viable tumor cells. Moreover, these mice lost their capability to develop any immune resistance even after an appropriate immunization procedure. The immunodepression induced by such a pretreatment regimen was specific for X5563 tumor. While no suppressor cell activity was detected in the above pretreated mice, serum factor(s) from these mice was virtually responsible for this suppression. When the serum factor mediating this tumor-specific suppression was fractionated on the Sephadex G-200 column, the suppressive activity was found in albumin-corresponding fraction, free of any immunoglobulin component. In contrast, in MM102 mammary tumor system, in which immune resistance is solely mediated by tumor-specific antibody, the pretreatment with x-irradiated MM102 cells augmented the induction of anti-tumor immunity. These results indicate that while tumor antigens given in the form of x-irradiated tumor cells suppress the induction of killer T cell-mediated immunity in one system, the same presensitization regimen of tumor antigens augments the antibody-mediated immunity in another system, thus giving a divergent effect on the distinct effector mechanisms of syngeneic tumor immunity. (author)

  18. 78 FR 40515 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2013-07-05

    ... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-071] Privacy Act of 1974; Privacy Act System of Records AGENCY: National Aeronautics and Space Administration (NASA). ACTION: Notice of Privacy Act system of records. SUMMARY: Each Federal agency is required by the Privacy Act of 1974 to publish...

  19. Dynamic analysis of a 6 DOF CKCM robot end-effector for dual-arm telerobot systems

    Science.gov (United States)

    Nguyen, Charles C.; Pooran, Farhad J.

    1989-01-01

    A dynamical analysis is performed for a six DOF robot end-effector built to study telerobotic service and maintenance of NASA hardwares in space. The design of the end-effector is based on the concept of closed-kinematic chain mechanism capable of performing precise motion in a small workspace. After presenting a closed-form solution for the inverse kinematic problem, the Lagrangian approach is used to derive a set of equations of motion for the end-effector where the generalized coordinates are selected to be the Cartesian coordinates. A computer simulation study shows that the centrifugal and Coriolis terms can be neglected for slow motion.

  20. ACT OF IMPLEMENTATION OF TOYOTA SYSTEM

    OpenAIRE

    Damjan Stanojević; Slobodan Stefanović; Branislav Stanisavljević; Dragoslav Ilić

    2013-01-01

    The production system developed by Toyota Motor Corporation was developed to provide best quality, lowest costs, and shortest lead time through the elimination of losts. Kiichiro Toyota, son of Sakichi and founder of the Toyota automobile factory, developed the concept of Just-in-Time in the 1930's. He decreed that by this act Toyota would contain no excess inventory and that Toyota would strive to work both in partnership with suppliers and level production. The Toyot...

  1. A survey of commercially available manipulators, end-effectors, and delivery systems for reactor decommissioning activities

    International Nuclear Information System (INIS)

    Henley, D.R.; Litka, T.J.

    1996-05-01

    Numerous nuclear facilities owned by the U.S. Department of Energy (DOE) are under consideration for decommissioning. Currently, there are no standardized, automated, remote systems designed to dismantle and thereby reduce the size of activated reactor components and vessels so that they can be packaged and shipped to disposal sites. Existing dismantling systems usually consist of customized, facility-specific tooling that has been developed to dismantle a specific reactor system. Such systems have a number of drawbacks. Generally, current systems cannot be disassembled, moved, and reused. Developing and deploying the tooling for current systems is expensive and time-consuming. In addition, the amount of manual work is significant because long-handled tools must be used; as a result, personnel are exposed to excessive radiation. A standardized, automated, remote system is therefore needed to deliver the tooling necessary to dismantle nuclear facilities at different locations. Because this system would be reusable, it would produce less waste. The system would also save money because of its universal design, and it would be more reliable than current systems

  2. Acute Hepatopancreatic Necrosis Disease-Causing Vibrio parahaemolyticus Strains Maintain an Antibacterial Type VI Secretion System with Versatile Effector Repertoires.

    Science.gov (United States)

    Li, Peng; Kinch, Lisa N; Ray, Ann; Dalia, Ankur B; Cong, Qian; Nunan, Linda M; Camilli, Andrew; Grishin, Nick V; Salomon, Dor; Orth, Kim

    2017-07-01

    Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. AHPND is caused by toxic strains of Vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins PirA vp /PirB vp To better understand the repertoire of virulence factors in AHPND-causing V. parahaemolyticus , we conducted a comparative analysis using the genome sequences of the clinical strain RIMD2210633 and of environmental non-AHPND and toxic AHPND isolates of V. parahaemolyticus Interestingly, we found that all of the AHPND strains, but none of the non-AHPND strains, harbor the antibacterial type VI secretion system 1 (T6SS1), which we previously identified and characterized in the clinical isolate RIMD2210633. This finding suggests that the acquisition of this T6SS might confer to AHPND-causing V. parahaemolyticus a fitness advantage over competing bacteria and facilitate shrimp infection. Additionally, we found highly dynamic effector loci in the T6SS1 of AHPND-causing strains, leading to diverse effector repertoires. Our discovery provides novel insights into AHPND-causing pathogens and reveals a potential target for disease control. IMPORTANCE Acute hepatopancreatic necrosis disease (AHPND) is a serious disease that has caused severe damage and significant financial losses to the global shrimp industry. To better understand and prevent this shrimp disease, it is essential to thoroughly characterize its causative agent, Vibrio parahaemolyticus Although the plasmid-encoded binary toxins PirA vp /PirB vp have been shown to be the primary cause of AHPND, it remains unknown whether other virulent factors are commonly present in V. parahaemolyticus and might play important roles during shrimp infection. Here, we analyzed the genome sequences of clinical, non-AHPND, and AHPND strains to characterize their repertoires of key virulence determinants. Our studies reveal that an antibacterial type

  3. Convergent Evolution of Pathogen Effectors toward Reactive Oxygen Species Signaling Networks in Plants

    Directory of Open Access Journals (Sweden)

    Nam-Soo Jwa

    2017-09-01

    Full Text Available Microbial pathogens have evolved protein effectors to promote virulence and cause disease in host plants. Pathogen effectors delivered into plant cells suppress plant immune responses and modulate host metabolism to support the infection processes of pathogens. Reactive oxygen species (ROS act as cellular signaling molecules to trigger plant immune responses, such as pathogen-associated molecular pattern (PAMP-triggered immunity (PTI and effector-triggered immunity. In this review, we discuss recent insights into the molecular functions of pathogen effectors that target multiple steps in the ROS signaling pathway in plants. The perception of PAMPs by pattern recognition receptors leads to the rapid and strong production of ROS through activation of NADPH oxidase Respiratory Burst Oxidase Homologs (RBOHs as well as peroxidases. Specific pathogen effectors directly or indirectly interact with plant nucleotide-binding leucine-rich repeat receptors to induce ROS production and the hypersensitive response in plant cells. By contrast, virulent pathogens possess effectors capable of suppressing plant ROS bursts in different ways during infection. PAMP-triggered ROS bursts are suppressed by pathogen effectors that target mitogen-activated protein kinase cascades. Moreover, pathogen effectors target vesicle trafficking or metabolic priming, leading to the suppression of ROS production. Secreted pathogen effectors block the metabolic coenzyme NADP-malic enzyme, inhibiting the transfer of electrons to the NADPH oxidases (RBOHs responsible for ROS generation. Collectively, pathogen effectors may have evolved to converge on a common host protein network to suppress the common plant immune system, including the ROS burst and cell death response in plants.

  4. Type VI Secretion Effectors: Methodologies and Biology

    Directory of Open Access Journals (Sweden)

    Yun-Wei Lien

    2017-06-01

    Full Text Available The type VI secretion system (T6SS is a nanomachine deployed by many Gram-negative bacteria as a weapon against eukaryotic hosts or prokaryotic competitors. It assembles into a bacteriophage tail-like structure that can transport effector proteins into the environment or target cells for competitive survival or pathogenesis. T6SS effectors have been identified by a variety of approaches, including knowledge/hypothesis-dependent and discovery-driven approaches. Here, we review and discuss the methods that have been used to identify T6SS effectors and the biological and biochemical functions of known effectors. On the basis of the nature and transport mechanisms of T6SS effectors, we further propose potential strategies that may be applicable to identify new T6SS effectors.

  5. 76 FR 64112 - Privacy Act of 1974; Privacy Act System of Records Appendices

    Science.gov (United States)

    2011-10-17

    ... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (11-091)] Privacy Act of 1974; Privacy Act...: Revisions of NASA Appendices to Privacy Act System of Records. SUMMARY: Notice is hereby given that NASA is... Privacy Act of 1974. This notice publishes those amendments as set forth below under the caption...

  6. The Salmonella SPI2 effector SseI mediates long-term systemic infection by modulating host cell migration.

    Directory of Open Access Journals (Sweden)

    Laura M McLaughlin

    2009-11-01

    Full Text Available Host-adapted strains of Salmonella enterica cause systemic infections and have the ability to persist systemically for long periods of time despite the presence of a robust immune response. Chronically infected hosts are asymptomatic and transmit disease to naïve hosts via fecal shedding of bacteria, thereby serving as a critical reservoir for disease. We show that the bacterial effector protein SseI (also called SrfH, which is translocated into host cells by the Salmonella Pathogenicity Island 2 (SPI2 type III secretion system (T3SS, is required for Salmonella typhimurium to maintain a long-term chronic systemic infection in mice. SseI inhibits normal cell migration of primary macrophages and dendritic cells (DC in vitro, and such inhibition requires the host factor IQ motif containing GTPase activating protein 1 (IQGAP1, an important regulator of cell migration. SseI binds directly to IQGAP1 and co-localizes with this factor at the cell periphery. The C-terminal domain of SseI is similar to PMT/ToxA, a bacterial toxin that contains a cysteine residue (C1165 that is critical for activity. Mutation of the corresponding residue in SseI (C178A eliminates SseI function in vitro and in vivo, but not binding to IQGAP1. In addition, infection with wild-type (WT S. typhimurium suppressed DC migration to the spleen in vivo in an SseI-dependent manner. Correspondingly, examination of spleens from mice infected with WT S. typhimurium revealed fewer DC and CD4(+ T lymphocytes compared to mice infected with Delta sseI S. typhimurium. Taken together, our results demonstrate that SseI inhibits normal host cell migration, which ultimately counteracts the ability of the host to clear systemic bacteria.

  7. Origins of immunity: transcription factors and homologues of effector genes of the vertebrate immune system expressed in sea urchin coelomocytes.

    Science.gov (United States)

    Pancer, Z; Rast, J P; Davidson, E H

    1999-08-01

    Echinoderms share common ancestry with the chordates within the deuterostome clade. Molecular features that are shared between their immune systems and that of mammals thus illuminate the basal genetic framework on which these immune systems have been constructed during evolution. The immune effector cells of sea urchins are the coelomocytes, whose primary function is protection against invasive marine pathogens; here we identify six genes expressed in coelomocytes, homologues of which are also expressed in cells of the mammalian immune system. Three coelomocyte genes reported here encode transcription factors. These are an NFKB homologue (SpNFKB); a GATA-2/3 homologue (SpGATAc); and a runt domain factor (SpRunt-1). All three of these coelomocyte genes respond sharply to bacterial challenge: SpNFKB and SpRunt-1 genes are rapidly up-regulated, while transcripts of SpGATAc factor disappear within hours of injection of bacteria. Sham injection also activates SpNFKB and SpRunt, though with slower kinetics, but does not affect SpGATAc levels. Another gene, SpHS, encodes a protein related to the signal transduction intermediate HS1 of lymphoid cells. Two other newly discovered genes, SpSRCR1 and SpSRCR5, encode proteins featuring SRCR repeats. These genes are members of a complex family of SRCR genes all expressed specifically in coelomocytes. The SRCR repeats most closely resemble those of mammalian macrophage scavenger receptors. Remarkably, each individual sea urchin expresses a specific pattern of SRCR genes. Our results imply some shared immune functions and more generally, a shared regulatory architecture which underlies immune system gene expression in all deuterostomes. We conclude that the vertebrate immune system has evolved by inserting new genes into old gene regulatory networks dedicated to immunity.

  8. Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape.

    Science.gov (United States)

    Brodmann, Maj; Dreier, Roland F; Broz, Petr; Basler, Marek

    2017-06-16

    Francisella tularensis is an intracellular pathogen that causes the fatal zoonotic disease tularaemia. Critical for its pathogenesis is the ability of the phagocytosed bacteria to escape into the cell cytosol. For this, the bacteria use a non-canonical type VI secretion system (T6SS) encoded on the Francisella pathogenicity island (FPI). Here we show that in F. novicida T6SS assembly initiates at the bacterial poles both in vitro and within infected macrophages. T6SS dynamics and function depends on the general purpose ClpB unfoldase, which specifically colocalizes with contracted sheaths and is required for their disassembly. T6SS assembly depends on iglF, iglG, iglI and iglJ, whereas pdpC, pdpD, pdpE and anmK are dispensable. Importantly, strains lacking pdpC and pdpD are unable to escape from phagosome, activate AIM2 inflammasome or cause disease in mice. This suggests that PdpC and PdpD are T6SS effectors involved in phagosome rupture.

  9. Manipulator systems for nuclear reactor decommissioning which can take end effectors

    International Nuclear Information System (INIS)

    Lampersberger, E.; Hell, W.; Vrba, H.

    1995-01-01

    The development of a remote-handling system on the basis of the new master-slave manipulator will decisively influence the possibilities of remote-controlled manipulation in nuclear technology under the premises of -high availability - universal range of application - a standard normal for industry - maintenance friendlyness. (orig.) [de

  10. [Neuropeptides, Cytokines and Thymus Peptides as Effectors of Interactions Between Thymus and Neuroendocrine System].

    Science.gov (United States)

    Torkhovskaya, T I; Belova, O V; Zimina, I V; Kryuchkova, A V; Moskvina, S N; Bystrova, O V; Arion, V Ya; Sergienko, V I

    2015-01-01

    The review presents data on mutual influence of nervous system and thymus, realized through the neuroendocrine-immune interactions. The pres- ence of adrenergic and peptidergic nerves in thymus creates conditions for implementation of the effect of neuropeptides secreted by them. These neuropeptides induce activation of thymus cells receptors and influence on the main processes in thymus, including T-lymphocyte maturation, cytokine and hormones production. In turn, thymuspeptides and/or cytokines, controlled by them, enter the brain and exert influence on neuro- nalfunction, which creates the basis for changes of behavior and homeostasis maintenance in response to infection. Ageing and some infectious, autoimmune, neurodegenerative and cancer diseases are accompanied by distortion of interactions between thymus and central nervous system. Mechanisms of signaling pathways, which determine these interactions, are not revealed yet, and their understanding will promote the development of effective therapeutic strategies.

  11. Construction of a large scale integrated map of macrophage pathogen recognition and effector systems

    Directory of Open Access Journals (Sweden)

    O'Sullivan Maire

    2010-05-01

    Full Text Available Abstract Background In an effort to better understand the molecular networks that underpin macrophage activation we have been assembling a map of relevant pathways. Manual curation of the published literature was carried out in order to define the components of these pathways and the interactions between them. This information has been assembled into a large integrated directional network and represented graphically using the modified Edinburgh Pathway Notation (mEPN scheme. Results The diagram includes detailed views of the toll-like receptor (TLR pathways, other pathogen recognition systems, NF-kappa-B, apoptosis, interferon signalling, MAP-kinase cascades, MHC antigen presentation and proteasome assembly, as well as selected views of the transcriptional networks they regulate. The integrated pathway includes a total of 496 unique proteins, the complexes formed between them and the processes in which they are involved. This produces a network of 2,170 nodes connected by 2,553 edges. Conclusions The pathway diagram is a navigable visual aid for displaying a consensus view of the pathway information available for these systems. It is also a valuable resource for computational modelling and aid in the interpretation of functional genomics data. We envisage that this work will be of value to those interested in macrophage biology and also contribute to the ongoing Systems Biology community effort to develop a standard notation scheme for the graphical representation of biological pathways.

  12. Nuclear lipids: key signaling effectors in the nervous system and other tissues.

    Science.gov (United States)

    Ledeen, Robert W; Wu, Gusheng

    2004-01-01

    Lipids have long been recognized as quantitatively minor components of the nucleus, where they were initially thought to have little functional importance; but they now command growing interest, with recognition of their diverse signaling and modulating properties in that organelle. This applies to the lipid-poor compartments of the nucleoplasm as well as the relatively lipid-rich nuclear envelope. Phosphoglycerides and sphingomyelin, as the predominant lipids, have attracted the most interest among researchers, but some of the less-abundant lipids such as gangliosides, sphingosine, and sphingosine phosphate are now becoming recognized as functionally important nuclear constituents. Among recent advances in this emerging field are detailed findings on the metabolic enzymes that synthesize and catabolize nuclear lipids; the fact that these are localized primarily within the nucleus itself indicates considerable autonomy with respect to lipid metabolism. Current studies suggest several key processes involving RNA and DNA reactivity that are dependent on these lipid-initiated events. Neural cell nuclei have been the subject of such investigations, with results that closely parallel the more numerous studies on nuclei of extraneural cells. This review attempts to outline some of the major findings on nuclear lipids of diverse cell types; results with nonneural nuclei will hopefully provide useful guideposts to further studies of neural systems.

  13. Ancestral acquisitions, gene flow and multiple evolutionary trajectories of the type three secretion system and effectors in Xanthomonas plant pathogens.

    Science.gov (United States)

    Merda, Déborah; Briand, Martial; Bosis, Eran; Rousseau, Céline; Portier, Perrine; Barret, Matthieu; Jacques, Marie-Agnès; Fischer-Le Saux, Marion

    2017-11-01

    Deciphering the evolutionary history and transmission patterns of virulence determinants is necessary to understand the emergence of novel pathogens. The main virulence determinant of most pathogenic proteobacteria is the type three secretion system (T3SS). The Xanthomonas genus includes bacteria responsible for numerous epidemics in agroecosystems worldwide and represents a major threat to plant health. The main virulence factor of Xanthomonas is the Hrp2 family T3SS; however, this system is not conserved in all strains and it has not been previously determined whether the distribution of T3SS in this bacterial genus has resulted from losses or independent acquisitions. Based on comparative genomics of 82 genome sequences representing the diversity of the genus, we have inferred three ancestral acquisitions of the Hrp2 cluster during Xanthomonas evolution followed by subsequent losses in some commensal strains and re-acquisition in some species. While mutation was the main force driving polymorphism at the gene level, interspecies homologous recombination of large fragments expanding through several genes shaped Hrp2 cluster polymorphism. Horizontal gene transfer of the entire Hrp2 cluster also occurred. A reduced core effectome composed of xopF1, xopM, avrBs2 and xopR was identified that may allow commensal strains overcoming plant basal immunity. In contrast, stepwise accumulation of numerous type 3 effector genes was shown in successful pathogens responsible for epidemics. Our data suggest that capacity to intimately interact with plants through T3SS would be an ancestral trait of xanthomonads. Since its acquisition, T3SS has experienced a highly dynamic evolutionary history characterized by intense gene flux between species that may reflect its role in host adaptation. © 2017 John Wiley & Sons Ltd.

  14. Tight Junction Disruption Induced by Type 3 Secretion System Effectors Injected by Enteropathogenic and Enterohemorrhagic Escherichia coli.

    Science.gov (United States)

    Ugalde-Silva, Paul; Gonzalez-Lugo, Octavio; Navarro-Garcia, Fernando

    2016-01-01

    The intestinal epithelium consists of a single cell layer, which is a critical selectively permeable barrier to both absorb nutrients and avoid the entry of potentially harmful entities, including microorganisms. Epithelial cells are held together by the apical junctional complexes, consisting of adherens junctions, and tight junctions (TJs), and by underlying desmosomes. TJs lay in the apical domain of epithelial cells and are mainly composed by transmembrane proteins such as occludin, claudins, JAMs, and tricellulin, that are associated with the cytoplasmic plaque formed by proteins from the MAGUK family, such as ZO-1/2/3, connecting TJ to the actin cytoskeleton, and cingulin and paracingulin connecting TJ to the microtubule network. Extracellular bacteria such as EPEC and EHEC living in the intestinal lumen inject effectors proteins directly from the bacterial cytoplasm to the host cell cytoplasm, where they play a relevant role in the manipulation of the eukaryotic cell functions by modifying or blocking cell signaling pathways. TJ integrity depends on various cell functions such as actin cytoskeleton, microtubule network for vesicular trafficking, membrane integrity, inflammation, and cell survival. EPEC and EHEC effectors target most of these functions. Effectors encoded inside or outside of locus of enterocyte effacement (LEE) disrupt the TJ strands. EPEC and EHEC exploit the TJ dynamics to open this structure, for causing diarrhea. EPEC and EHEC secrete effectors that mimic host proteins to manipulate the signaling pathways, including those related to TJ dynamics. In this review, we focus on the known mechanisms exploited by EPEC and EHEC effectors for causing TJ disruption.

  15. 77 FR 26027 - Privacy Act: Notification of a New Privacy Act System of Records, Veterans Homelessness...

    Science.gov (United States)

    2012-05-02

    ... a New Privacy Act System of Records, Veterans Homelessness Prevention Demonstration Evaluation Data... Homelessness Prevention Demonstration Evaluation Data Files (VHPD Data Files) system. The VHPD Data Files system will involve collaborative efforts needed to evaluate certain HUD homelessness prevention programs...

  16. 76 FR 11465 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-02

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2011-N-03] Privacy Act of 1974; System of Records AGENCY: Office of Inspector General, Federal Housing Finance Agency. ACTION: Notice of the Revision and Establishment of Privacy Act Systems of Records. SUMMARY: In accordance with the Privacy Act of 1974, as amended...

  17. 77 FR 74851 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-18

    ... FEDERAL DEPOSIT INSURANCE CORPORATION Privacy Act of 1974; System of Records AGENCY: Federal Deposit Insurance Corporation. ACTION: Notice to Delete a System of Records. SUMMARY: In accordance with the requirements of the Privacy Act of 1974, as amended (Privacy Act), the Federal Deposit Insurance...

  18. A Putative Type III Secretion System Effector Encoded by the MA20_12780 Gene in Bradyrhizobium japonicum Is-34 Causes Incompatibility with Rj4 Genotype Soybeans.

    Science.gov (United States)

    Tsurumaru, Hirohito; Hashimoto, Syougo; Okizaki, Kouhei; Kanesaki, Yu; Yoshikawa, Hirofumi; Yamakawa, Takeo

    2015-09-01

    The nodulation of Bradyrhizobium japonicum Is-34 is restricted by Rj4 genotype soybeans (Glycine max). To identify the genes responsible for this incompatibility, Tn5 mutants of B. japonicum Is-34 that were able to overcome this nodulation restriction were obtained. Analysis of the Tn5 mutants revealed that Tn5 was inserted into a region containing the MA20_12780 gene. In addition, direct disruption of this gene using marker exchange overcame the nodulation restriction by Rj4 genotype soybeans. The MA20_12780 gene has a tts box motif in its upstream region, indicating a possibility that this gene encodes a type III secretion system (T3SS) effector protein. Bioinformatic characterization revealed that the MA20_12780 protein contains the small ubiquitin-like modifier (SUMO) protease domain of the C48 peptidase (ubiquitin-like protease 1 [Ulp1]) family. The results of the present study indicate that a putative T3SS effector encoded by the MA20_12780 gene causes the incompatibility with Rj4 genotype soybeans, and they suggest the possibility that the nodulation restriction of B. japonicum Is-34 may be due to Rj4 genotype soybeans recognizing the putative T3SS effector (MA20_12780 protein) as a virulence factor. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  19. The Yersinia enterocolitica type 3 secretion system (T3SS) as toolbox for studying the cell biological effects of bacterial Rho GTPase modulating T3SS effector proteins.

    Science.gov (United States)

    Wölke, Stefan; Ackermann, Nikolaus; Heesemann, Jürgen

    2011-09-01

    The bacterial effector proteins IpgB(1) and IpgB(2) of Shigella and Map of Escherichia coli activate the Rho GTPases Rac1, RhoA and Cdc42, respectively, whereas YopE and YopT of Yersinia inhibit these Rho family GTPases. We established a Yersinia toolbox which allows to study the cellular effects of these effectors in different combinations in the context of Yersinia type 3 secretion system (Ysc)-T3SS-mediated injection into HeLa cells. For this purpose hybrid proteins were constructed by fusion of YopE with the effector protein of interest. As expected, injected hybrid proteins induced membrane ruffles and Yersinia uptake for IpgB(1) , stress fibres for IpgB(2) and microspikes for Map. By co-infection experiments we could demonstrate (i) IpgB(2) -mediated and ROCK-dependent inhibition of IpgB(1) -mediated Rac1 effects, (ii) YopT-mediated suppression of IpgB(1) -induced Yersinia invasion and (iii) failure of YopE-mediated suppression of IpgB(1) -induced Yersinia invasion, presumably due to preferential inhibition of RhoG by YopE GAP function. By infecting polarized MDCK cells we could demonstrate that Map or IpgB(1) but not IpgB(2) affects cell monolayer integrity. In summary, the Yersinia toolbox is suitable to study cellular effects of effector proteins of diverse bacterial species separately or in combination in the context of bacterial T3SS-mediated injection. © 2011 Blackwell Publishing Ltd.

  20. ACT OF IMPLEMENTATION OF TOYOTA SYSTEM

    Directory of Open Access Journals (Sweden)

    Damjan Stanojević

    2013-04-01

    Full Text Available The production system developed by Toyota Motor Corporation was developed to provide best quality, lowest costs, and shortest lead time through the elimination of losts. Kiichiro Toyota, son of Sakichi and founder of the Toyota automobile factory, developed the concept of Just-in-Time in the 1930's. He decreed that by this act Toyota would contain no excess inventory and that Toyota would strive to work both in partnership with suppliers and level production. The Toyota production system has been compared to squeezing water from a dry towel. Importance of Toyota sistem for production proces can sum up on the next way : Reducing costs includung the elimination of waste, With help without warehouse production – elimination the „excess inventory in production“, Reducing time of handwork using maxim „smallest posible of serve“, To reach a production without warehouse – its demand a production in small series, Its not neccessary to produce something else except that you realy need

  1. Computational prediction of secretion systems and secretomes of Brucella: identification of novel type IV effectors and their interaction with the host.

    Science.gov (United States)

    Sankarasubramanian, Jagadesan; Vishnu, Udayakumar S; Dinakaran, Vasudevan; Sridhar, Jayavel; Gunasekaran, Paramasamy; Rajendhran, Jeyaprakash

    2016-01-01

    Brucella spp. are facultative intracellular pathogens that cause brucellosis in various mammals including humans. Brucella survive inside the host cells by forming vacuoles and subverting host defence systems. This study was aimed to predict the secretion systems and the secretomes of Brucella spp. from 39 complete genome sequences available in the databases. Furthermore, an attempt was made to identify the type IV secretion effectors and their interactions with host proteins. We predicted the secretion systems of Brucella by the KEGG pathway and SecReT4. Brucella secretomes and type IV effectors (T4SEs) were predicted through genome-wide screening using JVirGel and S4TE, respectively. Protein-protein interactions of Brucella T4SEs with their hosts were analyzed by HPIDB 2.0. Genes coding for Sec and Tat pathways of secretion and type I (T1SS), type IV (T4SS) and type V (T5SS) secretion systems were identified and they are conserved in all the species of Brucella. In addition to the well-known VirB operon coding for the type IV secretion system (T4SS), we have identified the presence of additional genes showing homology with T4SS of other organisms. On the whole, 10.26 to 14.94% of total proteomes were found to be either secreted (secretome) or membrane associated (membrane proteome). Approximately, 1.7 to 3.0% of total proteomes were identified as type IV secretion effectors (T4SEs). Prediction of protein-protein interactions showed 29 and 36 host-pathogen specific interactions between Bos taurus (cattle)-B. abortus and Ovis aries (sheep)-B. melitensis, respectively. Functional characterization of the predicted T4SEs and their interactions with their respective hosts may reveal the secrets of host specificity of Brucella.

  2. 77 FR 15143 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-03-14

    ... RECOVERY ACCOUNTABILITY AND TRANSPARENCY BOARD [Doc. No. 12-002] Privacy Act of 1974; System of... Privacy Act system of records. SUMMARY: The Recovery Accountability and Transparency Board (Board) is... classification, system location, storage, retrievability, safeguards, retention and disposal, and system manager...

  3. 75 FR 15694 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-03-30

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This... John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The Defense Logistics Agency proposes to delete a system of records notice from its inventory of record systems subject to the Privacy Act of 1974...

  4. 75 FR 19626 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-15

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This.... Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The Agency proposes to delete a system of records notice in its inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended...

  5. 75 FR 20579 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-20

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This.... Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The Agency proposes to delete two system of records notices in its inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended...

  6. 75 FR 47797 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-09

    ... existing inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended... Logistics Agency, ATTN: DGA, 8725 John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The Agency proposes to delete a system of records notice in its inventory of record systems subject to the Privacy Act...

  7. 75 FR 19625 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-15

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This... John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The Agency proposes to delete a system of records notice in its inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C...

  8. 77 FR 40921 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-07-11

    ... Changes to USPS Privacy Act Systems of Records The Customer Experience Improvement Project enables a...[supreg] is proposing to modify a Customer Privacy Act System of Records. These modifications reflect the addition of language preferences selected by the customer and an update to the system manager's title...

  9. Impact of end effector technology on telemanipulation performance

    Science.gov (United States)

    Bejczy, A. K.; Szakaly, Z.; Ohm, T.

    1990-01-01

    Generic requirements for end effector design are briefly summarized as derived from generic functional and operational requirements. Included is a brief summary of terms and definitions related to end effector technology. The second part contains a brief overview of end effector technology work as JPL during the past ten years, with emphasis on the evolution of new mechanical, sensing and control capabilities of end effectors. The third and major part is devoted to the description of current end effector technology. The ongoing work addresses mechanical, sensing and control details with emphasis on mechanical ruggedness, increased resolution in sensing, and close electronic and control integration with overall telemanipulator control system.

  10. Intraspecies Competition in Serratia marcescens Is Mediated by Type VI-Secreted Rhs Effectors and a Conserved Effector-Associated Accessory Protein.

    Science.gov (United States)

    Alcoforado Diniz, Juliana; Coulthurst, Sarah J

    2015-07-01

    The type VI secretion system (T6SS) is widespread in Gram-negative bacteria and can deliver toxic effector proteins into eukaryotic cells or competitor bacteria. Antibacterial T6SSs are increasingly recognized as key mediators of interbacterial competition and may contribute to the outcome of many polymicrobial infections. Multiple antibacterial effectors can be delivered by these systems, with diverse activities against target cells and distinct modes of secretion. Polymorphic toxins containing Rhs repeat domains represent a recently identified and as-yet poorly characterized class of T6SS-dependent effectors. Previous work had revealed that the potent antibacterial T6SS of the opportunistic pathogen Serratia marcescens promotes intraspecies as well as interspecies competition (S. L. Murdoch, K. Trunk, G. English, M. J. Fritsch, E. Pourkarimi, and S. J. Coulthurst, J Bacteriol 193:6057-6069, 2011, http://dx.doi.org/10.1128/JB.05671-11). In this study, two new Rhs family antibacterial effectors delivered by this T6SS have been identified. One of these was shown to act as a DNase toxin, while the other contains a novel, cytoplasmic-acting toxin domain. Importantly, using S. marcescens, it has been demonstrated for the first time that Rhs proteins, rather than other T6SS-secreted effectors, can be the primary determinant of intraspecies competition. Furthermore, a new family of accessory proteins associated with T6SS effectors has been identified, exemplified by S. marcescens EagR1, which is specifically required for deployment of its associated Rhs effector. Together, these findings provide new insight into how bacteria can use the T6SS to deploy Rhs-family effectors and mediate different types of interbacterial interactions. Infectious diseases caused by bacterial pathogens represent a continuing threat to health and economic prosperity. To counter this threat, we must understand how such organisms survive and prosper. The type VI secretion system is a weapon that

  11. 76 FR 28002 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-05-13

    ... existing inventory of records systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES..., Headquarters Defense Logistics Agency, ATTN: DGA, 8725 John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060... is being deleted from the DLA inventory of Privacy Act systems of records. Deletion: S170.02...

  12. 77 FR 6543 - Privacy Act of 1974: Systems of Records

    Science.gov (United States)

    2012-02-08

    .... 120203097-2097-01] RIN 0660-XA26 Privacy Act of 1974: Systems of Records AGENCY: National Telecommunications... of Commerce publishes this notice to announce the deletion of a Privacy Act System of Records entitled, COMMERCE/ NTIA-1 ``Applications Related to Coupons for Digital-to-Analog Converter Boxes.'' The...

  13. 78 FR 31905 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-28

    ... Finance and Accounting Service, Freedom of Information/Privacy Act Program Manager, Corporate... of the Secretary Privacy Act of 1974; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting...

  14. 76 FR 53454 - Privacy Act System of Records

    Science.gov (United States)

    2011-08-26

    ... 1974, as amended (``Privacy Act''), 5 U.S.C. 552a, the FCC proposes to add one new, consolidated system... Commission (FCC, Commission, or the Agency). ACTION: Notice; one new Privacy Act system of records; two...,'' and also add new and updated information that pertains to the mission and activities of the FCC's...

  15. 75 FR 77872 - Privacy Act System of Records

    Science.gov (United States)

    2010-12-14

    ... (``Privacy Act''), 5 U.S.C. 552a, the FCC proposes to add one new, consolidated system of records, FCC/EB-5... Commission (FCC, Commission, or the Agency). ACTION: Notice; one new Privacy Act system of records; two.../EB-1, ``Violators Files,'' and FCC/EB-3, ``Investigations and Hearings,'' and also add new and...

  16. 78 FR 65644 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-01

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2013-N-14] Privacy Act of 1974; System of Records AGENCY: Office of Inspector General, Federal Housing Finance Agency. ACTION: Notice of the creation of a new... Finance Agency (FHFA-OIG) gives notice of the creation of a new Privacy Act system of records (SOR) for...

  17. 77 FR 73464 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-10

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2012-N-18] Privacy Act of 1974; System of Records AGENCY: Federal Housing Finance Agency. ACTION: Notice of proposed establishment of new system of records; Request....S.C. 552a (Privacy Act), the Federal Housing Finance Agency (FHFA) gives notice of and requests...

  18. 75 FR 71092 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-22

    ... Incident-Based Reporting System (DIBRS); 18 U.S.C. 922 note, The Brady Handgun Violence Prevention Act; 28... system: Storage: Delete entry and replace with ``Electronic storage media and file folders... (DIBRS); 18 U.S.C. 922 note, The Brady Handgun Violence Prevention Act; 28 U.S.C. 534 note, Uniform...

  19. Integration of Fiber-Optic Sensor Arrays into a Multi-Modal Tactile Sensor Processing System for Robotic End-Effectors

    Science.gov (United States)

    Kampmann, Peter; Kirchner, Frank

    2014-01-01

    With the increasing complexity of robotic missions and the development towards long-term autonomous systems, the need for multi-modal sensing of the environment increases. Until now, the use of tactile sensor systems has been mostly based on sensing one modality of forces in the robotic end-effector. The use of a multi-modal tactile sensory system is motivated, which combines static and dynamic force sensor arrays together with an absolute force measurement system. This publication is focused on the development of a compact sensor interface for a fiber-optic sensor array, as optic measurement principles tend to have a bulky interface. Mechanical, electrical and software approaches are combined to realize an integrated structure that provides decentralized data pre-processing of the tactile measurements. Local behaviors are implemented using this setup to show the effectiveness of this approach. PMID:24743158

  20. Integration of fiber-optic sensor arrays into a multi-modal tactile sensor processing system for robotic end-effectors.

    Science.gov (United States)

    Kampmann, Peter; Kirchner, Frank

    2014-04-16

    With the increasing complexity of robotic missions and the development towards long-term autonomous systems, the need for multi-modal sensing of the environment increases. Until now, the use of tactile sensor systems has been mostly based on sensing one modality of forces in the robotic end-effector. The use of a multi-modal tactile sensory system is motivated, which combines static and dynamic force sensor arrays together with an absolute force measurement system. This publication is focused on the development of a compact sensor interface for a fiber-optic sensor array, as optic measurement principles tend to have a bulky interface. Mechanical, electrical and software approaches are combined to realize an integrated structure that provides decentralized data pre-processing of the tactile measurements. Local behaviors are implemented using this setup to show the effectiveness of this approach.

  1. Integration of Fiber-Optic Sensor Arrays into a Multi-Modal Tactile Sensor Processing System for Robotic End-Effectors

    Directory of Open Access Journals (Sweden)

    Peter Kampmann

    2014-04-01

    Full Text Available With the increasing complexity of robotic missions and the development towards long-term autonomous systems, the need for multi-modal sensing of the environment increases. Until now, the use of tactile sensor systems has been mostly based on sensing one modality of forces in the robotic end-effector. The use of a multi-modal tactile sensory system is motivated, which combines static and dynamic force sensor arrays together with an absolute force measurement system. This publication is focused on the development of a compact sensor interface for a fiber-optic sensor array, as optic measurement principles tend to have a bulky interface. Mechanical, electrical and software approaches are combined to realize an integrated structure that provides decentralized data pre-processing of the tactile measurements. Local behaviors are implemented using this setup to show the effectiveness of this approach.

  2. Advanced Communications Technology Satellite (ACTS) and potential system applications

    Science.gov (United States)

    Wright, David L.; Balombin, Joseph R.; Sohn, Philip Y.

    1990-01-01

    A description of the advanced communications technology satellite (ACTS) system is given with special emphasis on the communication characteristics. Potential satellite communications scenarios, including future operational ACTS-like satellite systems, are discussed. The description of the ACTS system updates previously published ACTS system references. Detailed information on items such as experimental ground stations is presented. The potential services can be generically described as voice, video, and data services. The implementation of these services on future operational ACTS-like systems can lead to unique quality, flexibility, and capacity characteristics at lower service costs. The specific service applications that could be supported range from low to high data rates and include both domestic and international applications.

  3. 76 FR 53420 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-08-26

    ... Management (OPM) government-wide Privacy Act systems of records. OPM has government-wide responsibility for various systems of records maintained on Federal civilian employees. BILLING CODE 5001-06-P ...

  4. 77 FR 69898 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2012-11-21

    ... System of Records AGENCY: National Aeronautics and Space Administration (NASA). ACTION: Notice of... Privacy Act of 1974 (5 U.S.C. 552a), the National Aeronautics and Space Administration is issuing public... Information System (EOSDIS) User Information/GSFC 51EUI. This notice publishes updates of this system of...

  5. Pneumatic inflatable end effector

    Science.gov (United States)

    Clark, K. H.; Johnston, J. D. (Inventor)

    1981-01-01

    The invention relates to an end effector device for robot or teleoperated type space vehicle which includes an inflatable balloon member carried on the end of tubular member which has a hollow center or conduit through which a suitable pressurized fluid is supplied. The device may be inserted into a variety of shaped openings or truss-type structures for handling in space.

  6. Functional analysis of Hyaloperonospora arabidopsidis RXLR effectors.

    Directory of Open Access Journals (Sweden)

    Michiel J C Pel

    Full Text Available The biotrophic plant pathogen Hyaloperonospora arabidopsidis produces a set of putative effector proteins that contain the conserved RXLR motif. For most of these RXLR proteins the role during infection is unknown. Thirteen RXLR proteins from H. arabidopsidis strain Waco9 were analyzed for sequence similarities and tested for a role in virulence. The thirteen RXLR proteins displayed conserved N-termini and this N-terminal conservation was also found in the 134 predicted RXLR genes from the genome of H. arabidopsidis strain Emoy2. To investigate the effects of single RXLR effector proteins on plant defense responses, thirteen H. arabidopsidis Waco9 RXLR genes were expressed in Arabidopsis thaliana. Subsequently, these plants were screened for altered susceptibility to the oomycetes H. arabidopsidis and Phytophthora capsici, and the bacterial pathogen Pseudomonas syringae. Additionally, the effect of the RXLR proteins on flg22-triggered basal immune responses was assessed. Multifactorial analysis of results collated from all experiments revealed that, except for RXLR20, all RXLR effector proteins tested affected plant immunity. For RXLR9 this was confirmed using a P. syringae ΔCEL-mediated effector delivery system. Together, the results show that many H. arabidopsidis RXLR effectors have small effects on the plant immune response, suggesting that suppression of host immunity by this biotrophic pathogen is likely to be caused by the combined actions of effectors.

  7. Two-component regulatory system ActS/ActR is required for Sinorhizobium meliloti adaptation to oxidative stress.

    Science.gov (United States)

    Tang, Guirong; Wang, Sunjun; Lu, Dawei; Huang, Leqi; Li, Ningning; Luo, Li

    2017-05-01

    The two-component system ActS/ActR plays important roles in bacterial adaptation to abiotic stress, including acid tolerance and oxidant resistance. However, the underlying regulatory mechanism is not clear. In this study, we found that the ActS/ActR system is required for adaptation to oxidative stress by regulating the transcription of the genes actR, katB, gshA and gshB1. The actS and actR mutants were sensitive to low pH and oxidants such as H 2 O 2 , oxidized glutathione (GSSG) and sodium nitroprusside (SNP). The expression of actR by using a plasmid rescued the defect of SNP sensitivity for all actS and actR mutants. The expression of actS and actR were suppressed by treatment with H 2 O 2 . The expression of actS, actR, oxyR, katA and katB was required for ActS and ActR under normal conditions. The induction of katB, gshA and gshB1 depended on ActS and ActR during treatment with H 2 O 2 and SNP. Our findings revealed that the ActS/ActR system is a key redox regulator in S. meliltoi and provides a new cue to understanding Rhizobium-legume symbiosis. Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.

  8. Activation of the human mirror neuron system during the observation of the manipulation of virtual tools in the absence of a visible effector limb.

    Science.gov (United States)

    Modroño, Cristián; Navarrete, Gorka; Rodríguez-Hernández, Antonio F; González-Mora, José L

    2013-10-25

    This work explores the mirror neuron system activity produced by the observation of virtual tool manipulations in the absence of a visible effector limb. Functional MRI data was obtained from healthy right-handed participants who manipulated a virtual paddle in the context of a digital game and watched replays of their actions. The results show how action observation produced extended bilateral activations in the parietofrontal mirror neuron system. At the same time, three regions in the left hemisphere (in the primary motor and the primary somatosensory cortex, the supplementary motor area and the dorsolateral prefrontal cortex) showed a reduced BOLD, possibly related with the prevention of inappropriate motor execution. These results can be of interest for researchers and developers working in the field of action observation neurorehabilitation. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  9. 76 FR 80344 - Privacy Act of 1974: Systems of Records

    Science.gov (United States)

    2011-12-23

    .... 111215762-1761-01] RIN 0605-XA42 Privacy Act of 1974: Systems of Records AGENCY: National Telecommunications...: In accordance with the Privacy Act (5 U.S.C. 552a(e)(4) and (11)), the Department of Commerce is... Related to Coupons for Digital-to-Analog Converter Boxes.'' The Digital-to-Analog Converter Box Program...

  10. 76 FR 45783 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-01

    ... DEPARTMENT OF DEFENSE Department of the Army [Docket ID USA-2011-0018] Privacy Act of 1974; System... offense. To the Immigration and Naturalization Service, Department of Justice, for use in alien admission and naturalization inquiries conducted under Section 105 of the Immigration and Naturalization Act of...

  11. 76 FR 64115 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2011-10-17

    ... System of Records AGENCY: National Aeronautics and Space Administration (NASA). ACTION: Notice of... Privacy Act of 1974 (5 U.S.C. 552a), the National Aeronautics and Space Administration is issuing public... of those systems of records as set forth below under the caption SUPPLEMENTARY INFORMATION. DATES...

  12. 78 FR 77503 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2013-12-23

    ... System of Records AGENCY: National Aeronautics And Space Administration (NASA). ACTION: Notice of... Privacy Act of 1974 (5 U.S.C. 552a), the National Aeronautics and Space Administration is issuing public... of those systems of records as set forth below under the caption SUPPLEMENTARY INFORMATION. DATES...

  13. 76 FR 67763 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2011-11-02

    ... System of Records AGENCY: National Aeronautics and Space Administration (NASA). ACTION: Notice of... Privacy Act of 1974 (5 U.S.C. 552a), the National Aeronautics and Space Administration is issuing public notice of its proposal to modify its existing system of records entitled ``NASA Freedom of Information...

  14. 76 FR 64114 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2011-10-17

    ... System of Records AGENCY: National Aeronautics and Space Administration (NASA). ACTION: Notice of... Privacy Act of 1974 (5 U.S.C. 552a), the National Aeronautics and Space Administration is issuing public..., [email protected] . SUPPLEMENTARY INFORMATION: The NASA system of records previously titled and...

  15. 77 FR 69444 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-11-19

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a system of records. SUMMARY: The Defense Finance and Accounting Service is amending a system of records... INFORMATION: The Defense Finance and Accounting Service systems of records notices subject to the Privacy Act...

  16. 78 FR 47309 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-08-05

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records... Defense Finance and Accounting Service systems of records notices subject to the Privacy Act of 1974 (5 U...

  17. 78 FR 27195 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-09

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to delete two Systems of Records. SUMMARY: The Defense Finance and Accounting Service is deleting two systems of records...: The Defense Finance and Accounting Service systems of records notices subject to the Privacy Act of...

  18. 78 FR 14281 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ...; System of Records AGENCY: Office of the Secretary of Defense, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting Service proposes to amend a system of records in its inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES: This...

  19. 78 FR 69392 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-19

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to delete a system of records. SUMMARY: The Defense Finance and Accounting Service is deleting a system of records... INFORMATION: The Defense Finance and Accounting Service systems of records notices subject to the Privacy Act...

  20. 78 FR 27194 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-09

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to delete two Systems of Records. SUMMARY: The Defense Finance and Accounting Service is deleting two systems of records...: The Defense Finance and Accounting Service systems of records notices subject to the Privacy Act of...

  1. 78 FR 35607 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-06-13

    ... Register Liaison Officer, Department of Defense. NM05211-1 System name: Privacy Act Request/Amendment Files and Tracking System (April 2, 2008, 73 FR 17959) Changes: * * * * * System location: Delete entry and... request filed; serial number of response letter; case file number. The system manager may require an...

  2. 76 FR 79216 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-21

    ... Prisons (Bureau) proposes to modify in part its system of records entitled ``Telephone Activity Record... Department of Justice. JUSTICE/BOP-011 SYSTEM NAME: Telephone Activity Record System. SECURITY CLASSIFICATION... DEPARTMENT OF JUSTICE [CPCLO Order No. 005-2011] Privacy Act of 1974; System of Records AGENCY...

  3. Live cell imaging reveals novel functions of Salmonella enterica SPI2-T3SS effector proteins in remodeling of the host cell endosomal system.

    Science.gov (United States)

    Rajashekar, Roopa; Liebl, David; Chikkaballi, Deepak; Liss, Viktoria; Hensel, Michael

    2014-01-01

    Intracellular Salmonella enterica induce a massive remodeling of the endosomal system in infected host cells. One dramatic consequence of this interference is the induction of various extensive tubular aggregations of membrane vesicles, and tubules positive for late endosomal/lysosomal markers are referred to as Salmonella-induced filaments or SIF. SIF are highly dynamic in nature with extension and collapse velocities of 0.4-0.5 µm x sec-1. The induction of SIF depends on the function of the Salmonella Pathogenicity Island 2 (SPI2) encoded type III secretion system (T3SS) and a subset of effector proteins. In this study, we applied live cell imaging and electron microscopy to analyze the role of individual effector proteins in SIF morphology and dynamic properties of SIF. SIF in cells infected with sifB, sseJ, sseK1, sseK2, sseI, sseL, sspH1, sspH2, slrP, steC, gogB or pipB mutant strains showed a morphology and dynamics comparable to SIF induced by WT Salmonella. SIF were absent in cells infected with the sifA-deficient strain and live cell analyses allowed tracking of the loss of the SCV membrane of intracellular sifA Salmonella. In contrast to analyses in fixed cells, in living host cells SIF induced by sseF- or sseG-deficient strains were not discontinuous, but rather continuous and thinner in diameter. A very dramatic phenotype was observed for the pipB2-deficient strain that induced very bulky, non-dynamic aggregations of membrane vesicles. Our study underlines the requirement of the study of Salmonella-host interaction in living systems and reveals new phenotypes due to the intracellular activities of Salmonella.

  4. The Type III Secretion System Effector SeoC of Salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis.

    Science.gov (United States)

    Pollard, Dominic J; Young, Joanna C; Covarelli, Valentina; Herrera-León, Silvia; Connor, Thomas R; Fookes, Maria; Walker, Danielle; Echeita, Aurora; Thomson, Nicholas R; Berger, Cedric N; Frankel, Gad

    2016-12-01

    Salmonella species utilize type III secretion systems (T3SSs) to translocate effectors into the cytosol of mammalian host cells, subverting cell signaling and facilitating the onset of gastroenteritis. In this study, we compared a draft genome assembly of Salmonella enterica subsp. salamae strain 3588/07 against the genomes of S. enterica subsp. enterica serovar Typhimurium strain LT2 and Salmonella bongori strain 12419. S. enterica subsp. salamae encodes the Salmonella pathogenicity island 1 (SPI-1), SPI-2, and the locus of enterocyte effacement (LEE) T3SSs. Though several key S Typhimurium effector genes are missing (e.g., avrA, sopB, and sseL), S. enterica subsp. salamae invades HeLa cells and contains homologues of S. bongori sboK and sboC, which we named seoC SboC and SeoC are homologues of EspJ from enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), which inhibit Src kinase-dependent phagocytosis by ADP-ribosylation. By screening 73 clinical and environmental Salmonella isolates, we identified EspJ homologues in S. bongori, S. enterica subsp. salamae, and Salmonella enterica subsp. arizonae The β-lactamase TEM-1 reporter system showed that SeoC is translocated by the SPI-1 T3SS. All the Salmonella SeoC/SboC homologues ADP-ribosylate Src E310 in vitro Ectopic expression of SeoC/SboC inhibited phagocytosis of IgG-opsonized beads into Cos-7 cells stably expressing green fluorescent protein (GFP)-FcγRIIa. Concurrently, S. enterica subsp. salamae infection of J774.A1 macrophages inhibited phagocytosis of beads, in a seoC-dependent manner. These results show that S. bongori, S. enterica subsp. salamae, and S. enterica subsp. arizonae share features of the infection strategy of extracellular pathogens EPEC and EHEC and shed light on the complexities of the T3SS effector repertoires of Enterobacteriaceae. Copyright © 2016 Pollard et al.

  5. Functional differences and interactions between the Escherichia coli type III secretion system effectors NleH1 and NleH2.

    Science.gov (United States)

    Pham, Thanh H; Gao, Xiaofei; Tsai, Karen; Olsen, Rachel; Wan, Fengyi; Hardwidge, Philip R

    2012-06-01

    The human pathogens enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC), as well as the related mouse pathogen Citrobacter rodentium, utilize a type III secretion system (T3SS) to inject multiple effector proteins into host cells. The E. coli O157:H7 strain EDL933 carries two copies of non-locus of enterocyte effacement (LEE)-encoded protein H, designated NleH1 and NleH2, both of which bind to the human ribosomal protein S3 (RPS3), a subunit of NF-κB transcriptional complexes. In this study, we describe significant functional differences between NleH1 and NleH2 in their ability to regulate the host NF-κB pathway. We show that the EHEC and EPEC NleH effectors are functionally equivalent in their ability to affect RPS3 nuclear translocation. NleH1, but not NleH2, inhibited NF-κB activity without altering the kinetics of IκBα phosphorylation/degradation. We also determined that the class I PSD-95/Disc Large/ZO-1 (PDZ)-binding domain of NleH was important for its activity in the NF-κB pathway. In addition to binding RPS3, we found that NleH1 and NleH2 are able to bind to each other in vitro and in vivo, suggesting an additional mechanism by which the E. coli NleH effectors may regulate the extent and duration of NF-κB activation after their T3SS-dependent translocation. We also performed mouse infection experiments and established that mouse mortality and Citrobacter colonization were reduced in mice infected with ΔnleH. Complementing ΔnleH with NleH1 restored Citrobacter virulence and colonization to wild-type levels, whereas complementing with NleH2 reduced them. Taken together, our data show that NleH1 and NleH2 have pronounced functional differences in their ability to alter host transcriptional responses to bacterial infection.

  6. 76 FR 63611 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-13

    ... existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES... Declassification Agency, 7701 Telegraph Road, Casey Building, Suite 144, Alexandria, VA 22325-3905, or by phone at... proposes to delete one system of records notice from its inventory of record systems subject to the Privacy...

  7. 77 FR 1487 - Privacy Act System of Records

    Science.gov (United States)

    2012-01-10

    ... proposes to add one new system of records, FCC/PSHSB-2, ``PSHSB Contact Database.'' The FCC's Public Safety... Commission (FCC or Commission). ACTION: Notice; one new Privacy Act system(s) of records. SUMMARY: Pursuant... submit voluntarily via one or more of the PSHSB's customer comment formats: PSHSB's (electronic) Web page...

  8. 78 FR 66709 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2013-11-06

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2013-N-15] Privacy Act of 1974; Systems of Records AGENCY: Federal Housing Finance Agency. ACTION: Notice of proposed revision of an existing system of records and... SYSTEM: Current and former Federal Housing Finance Agency (FHFA) employees and contractor personnel...

  9. 75 FR 35028 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-21

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2010-N-07] Privacy Act of 1974; System of Records AGENCY: Federal Housing Finance Agency. ACTION: Notice of the establishment of a new system of records. SUMMARY: The Federal Housing Finance Agency (FHFA) is revising the proposed system of records notice that was...

  10. 76 FR 33286 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-06-08

    ... FEDERAL HOUSING FINANCE AGENCY [No. 2011-N-06] Privacy Act of 1974; System of Records AGENCY: Federal Housing Finance Agency. ACTION: Notice of the Establishment of New Systems of Records, Adoption of... Government-Wide Systems of Records previously adopted by the Federal Housing Finance Agency. SUMMARY: In...

  11. 76 FR 58788 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-09-22

    ...; Systems of Records AGENCY: Defense Finance and Accounting Service, Department of Defense, (DoD). ACTION: Notice to Add a System of Records. SUMMARY: The Defense Finance and Accounting Service (DFAS) is... Defense Finance and Accounting Service notices for systems of records subject to the Privacy Act of 1974...

  12. 76 FR 8352 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-02-14

    ... notices in its existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a... of Personnel Management, Defense Intelligence Agency, Washington headquarters adjudication facility... System (February 22, 1993, 58 FR 10562). Reason: The identification badge system does not contain...

  13. 76 FR 73604 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-29

    ... system of records notice in its existing inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on... Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd floor, Suite 02G09, Alexandria...

  14. 76 FR 43993 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-07-22

    ... Service is deleting a system of records notice from its existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action would be effective... submitting comments. Mail: Federal Docket Management System Office, 1160 Defense Pentagon, Washington, DC...

  15. The Aeromonas salmonicida subsp. salmonicida exoproteome: determination of the complete repertoire of Type-Three Secretion System effectors and identification of other virulence factors

    Science.gov (United States)

    2013-01-01

    Background Aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen of fisheries worldwide. Several virulence factors have been described, but the type-three secretion system (T3SS) is recognized as having a major effect on virulence by injecting effectors directly into fish cells. In this study we used high-throughput proteomics to display the differences between in vitro secretome of A. salmonicida wild-type (wt, hypervirulent, JF2267) and T3SS-deficient (isogenic ΔascV, extremely low-virulent, JF2747) strains in exponential and stationary phases of growth. Results Results confirmed the secretion of effectors AopH, AexT, AopP and AopO via T3SS, and for the first time demonstrated the impact of T3SS in secretion of Ati2, AopN and ExsE that are known as effectors in other pathogens. Translocators, needle subunits, Ati1, and AscX were also secreted in supernatants (SNs) dependent on T3SS. AopH, Ati2, AexT, AopB and AopD were in the top seven most abundant excreted proteins. EF-G, EF-Tu, DnaK, HtpG, PNPase, PepN and MdeA were moderately secreted in wt SNs and predicted to be putative T3 effectors by bioinformatics. Pta and ASA_P5G088 were increased in wt SNs and T3-associated in other bacteria. Ten conserved cytoplasmic proteins were more abundant in wt SNs than in the ΔascV mutant, but without any clear association to a secretion system. T1-secreted proteins were predominantly found in wt SNs: OmpAI, OmpK40, DegQ, insulinase ASA_0716, hypothetical ASA_0852 and ASA_3619. Presence of T3SS components in pellets was clearly decreased by ascV deletion, while no impact was observed on T1- and T2SS. Our results demonstrated that the ΔascV mutant strain excreted well-described (VapA, AerA, AerB, GCAT, Pla1, PlaC, TagA, Ahe2, GbpA and enolase) and yet uncharacterized potential toxins, adhesins and enzymes as much as or even more than the wt strain. Other putative important virulence factors were not detected. Conclusions We

  16. Cooperative Immune Suppression by Escherichia coli and Shigella Effector Proteins.

    Science.gov (United States)

    de Jong, Maarten F; Alto, Neal M

    2018-04-01

    The enteric attaching and effacing (A/E) pathogens enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) and the invasive pathogens enteroinvasive E. coli (EIEC) and Shigella encode type III secretion systems (T3SS) used to inject effector proteins into human host cells during infection. Among these are a group of effectors required for NF-κB-mediated host immune evasion. Recent studies have identified several effector proteins from A/E pathogens and EIEC/ Shigella that are involved in suppression of NF-κB and have uncovered their cellular and molecular functions. A novel mechanism among these effectors from both groups of pathogens is to coordinate effector function during infection. This cooperativity among effector proteins explains how bacterial pathogens are able to effectively suppress innate immune defense mechanisms in response to diverse classes of immune receptor signaling complexes (RSCs) stimulated during infection. Copyright © 2018 American Society for Microbiology.

  17. 76 FR 19307 - Privacy Act of 1974; Amendment of Privacy Act System of Records

    Science.gov (United States)

    2011-04-07

    ... (202) 260-3245. FOR FURTHER INFORMATION CONTACT: Karren Y. Alexander, Director, USDA Forest Service... acres of land, to meet the needs of present and future generations. The purpose of this system is to... purchase and wear the Forest Service uniform. Pursuant to the Privacy Act (5 U.S.C. 552a), the Forest...

  18. 76 FR 26714 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-05-09

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This..., Headquarters Defense Logistics Agency, ATTN: DGA, 8725 John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060... from the DLA inventory of systems of records. Service members seeking legal assistance are referred to...

  19. 75 FR 25856 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-10

    ... of Policy, Analysis and Research and the Division of Bank Regulation, Federal Housing Finance Agency... FEDERAL HOUSING FINANCE AGENCY [No. 2010-N-05] Privacy Act of 1974; System of Records AGENCY: Federal Housing Finance Agency. ACTION: Notice of the establishment of new systems of records. SUMMARY: In...

  20. 78 FR 14283 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Finance and Accounting Service, Freedom of Information/Privacy Act Program Manager, Corporate... a system of records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system... identifiers or contact information. FOR FURTHER INFORMATION CONTACT: Mr. Gregory L. Outlaw, Defense Finance...

  1. 77 FR 44207 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2012-07-27

    ... the Privacy Act. It is USAID's core financial management system and accounting system of record... Budget Execution, which are required to perform necessary accounting operations. Phoenix falls under... Payroll files for Personnel Services Contractors (PSC) and direct hires: name, social security number...

  2. 76 FR 10010 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-02-23

    ... inventory of records systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on March 25, 2011 unless comments are received... Management System Office, 1160 Defense Pentagon, Mailroom 3C843, Washington, DC 20301-1160. Instructions: All...

  3. 76 FR 28001 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-13

    ... existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on June 13, 2011 unless comments are....regulations.gov Follow the instructions for submitting comments. Mail: Federal Docket Management System Office...

  4. 76 FR 46756 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-03

    ... its existing inventory of records systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on September 2, 2011 unless... Docket Management System Office, 1160 Defense Pentagon, Washington, DC 20301-1160. Instructions: All...

  5. 75 FR 33794 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-15

    ... inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on July 15, 2010, unless comments are received... . Follow the instructions for submitting comments. Mail: Federal Docket Management System Office, 1160...

  6. 75 FR 30003 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-28

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on June 28, 2010 unless comments are received... . Follow the instructions for submitting comments. * Mail: Federal Docket Management System Office, 1160...

  7. 76 FR 22682 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-22

    ... inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on May 23, 2011 unless comments are received which... Docket Management System Office, 1160 Defense Pentagon, OSD Mailroom 3C843, Washington, DC 20301-1160...

  8. 77 FR 60400 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-03

    ... inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a(r)), as amended. DATES: This proposed action will be effective on November 5, 2012 unless comments are received which result in a... Management System Office, 4800 Mark Center Drive, East Tower, 2nd Floor, Suite 02G09, Alexandria, VA 22350...

  9. 75 FR 16760 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-02

    ... inventory of record systems subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on May 3, 2010 unless comments are received which... the instructions for submitting comments. * Mail: Federal Docket Management System Office, 1160...

  10. 77 FR 26260 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-03

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on June 4, 2012 unless comments are received which... the instructions for submitting comments. * Mail: Federal Docket Management System Office, 4800 Mark...

  11. 75 FR 6000 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-02-05

    ... inventory of records systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on March 8, 2010 unless comments are received... . Follow the instructions for submitting comments. * Mail: Federal Docket Management System Office, 1160...

  12. 75 FR 29728 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-27

    ... existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on June 28, 2010 unless comments are... . Follow the instructions for submitting comments. Mail: Federal Docket Management System Office, 1160...

  13. 78 FR 45185 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-26

    .... Complaints appearing to involve criminal wrongdoing will be referred to the Defense Criminal Investigative... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID DoD-2013-OS-0166] Privacy Act of 1974..., Defense Case Activity Tracking System (D-CATS), in its inventory of record systems subject to the Privacy...

  14. 77 FR 26258 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-03

    ... defense and foreign policy, to avoid interference during the conduct of criminal, civil, or administrative... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID DoD-2012-OS-0029] Privacy Act of 1974; System of Records AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice to Alter a System of Records...

  15. 77 FR 26256 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-03

    ... defense and foreign policy, to avoid interference during the conduct of criminal, civil, or administrative... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID: DoD-2012-OS-0033] Privacy Act of 1974; System of Records AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice to Alter a System of Records...

  16. 77 FR 77049 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-31

    ..., Air Force Privacy Act Office, Office of Warfighting Integration and Chief Information officer, ATTN... of 1974 (5 U.S.C. 552a), as amended, which requires the submission of a new or altered system report...). Reason The Innovative Development through Employee Awareness (IDEA) Program Data System (IPDS) has been...

  17. Strategy to develop and test a multi-function scarifier end effector with an integral conveyance system for waste tank remediation

    International Nuclear Information System (INIS)

    Bamberger, J.A.; Bates, J.M.; Keska, J.K.; Elmore, M.R.; Lombardo, N.J.

    1993-08-01

    This strategy plan describes a coupled analytical/experimental approach to develop a multi-functional scarifier end effector coupled with a pneumatic conveyance system to retrieve wastes from underground storage tanks. The scarifier uses ultra-high-pressure water jets to rubblize and entrain waste forms such as salt cake, sludge, and viscous liquid that can be transported pneumatically. The three waste types (hard, brittle, salt cake, viscous liquid, and deformable sludge) present increasingly complex challenges for scarification and pneumatic conveyance. Salt cake is anticipated to be the easiest to retrieve because (1) a theoretical model of hydraulic rock fracture can be applied to estimate jet performance to fracture salt cake, and (2) gas-solids transport correlations can be used to predict pneumatic transport. Deformable sludge is anticipated to be the most difficult to retrieve: no theories, correlations, or data exist to predict this performance. However order-of-magnitude gas-solid correlations indicate particulate wastes of prototypic density can be transported to a height of 20 m within allowable pressure limits provided that the volume fraction of the gaseous phase is kept above 95%. Viscous liquid is anticipated to be of intermediate complexity to retrieve. Phenomena that are expected to affect system performance are ranked. Experiments and analyses necessary to evaluate the effects of these phenomena are proposed. Subsequent strategies for experiment test plans, system deployment, and operation and control will need to be developed

  18. Acute up-regulation of the rat brain somatostatin receptor-effector system by leptin is related to activation of insulin signaling and may counteract central leptin actions.

    Science.gov (United States)

    Perianes-Cachero, A; Burgos-Ramos, E; Puebla-Jiménez, L; Canelles, S; Frago, L M; Hervás-Aguilar, A; de Frutos, S; Toledo-Lobo, M V; Mela, V; Viveros, M P; Argente, J; Chowen, J A; Arilla-Ferreiro, E; Barrios, V

    2013-11-12

    Leptin and somatostatin (SRIF) have opposite effects on food seeking and ingestive behaviors, functions partially regulated by the frontoparietal cortex and hippocampus. Although it is known that the acute suppression of food intake mediated by leptin decreases with time, the counter-regulatory mechanisms remain unclear. Our aims were to analyze the effect of acute central leptin infusion on the SRIF receptor-effector system in these areas and the implication of related intracellular signaling mechanisms in this response. We studied 20 adult male Wister rats including controls and those treated intracerebroventricularly with a single dose of 5 μg of leptin and sacrificed 1 or 6h later. Density of SRIF receptors was unchanged at 1h, whereas leptin increased the density of SRIF receptors at 6h, which was correlated with an elevated capacity of SRIF to inhibit forskolin-stimulated adenylyl cyclase activity in both areas. The functional capacity of SRIF receptors was unaltered as cell membrane levels of αi1 and αi2 subunits of G inhibitory proteins were unaffected in both brain areas. The increased density of SRIF receptors was due to enhanced SRIF receptor subtype 2 (sst2) protein levels that correlated with higher mRNA levels for this receptor. These changes in sst2 mRNA levels were concomitant with increased activation of the insulin signaling, c-Jun and cyclic AMP response element-binding protein (CREB); however, activation of signal transducer and activator of transcription 3 was reduced in the cortex and unchanged in the hippocampus and suppressor of cytokine signaling 3 remained unchanged in these areas. In addition, the leptin antagonist L39A/D40A/F41A blocked the leptin-induced changes in SRIF receptors, leptin signaling and CREB activation. In conclusion, increased activation of insulin signaling after leptin infusion is related to acute up-regulation of the SRIF receptor-effector system that may antagonize short-term leptin actions in the rat brain

  19. Genome-scale identification of Legionella pneumophila effectors using a machine learning approach.

    Science.gov (United States)

    Burstein, David; Zusman, Tal; Degtyar, Elena; Viner, Ram; Segal, Gil; Pupko, Tal

    2009-07-01

    A large number of highly pathogenic bacteria utilize secretion systems to translocate effector proteins into host cells. Using these effectors, the bacteria subvert host cell processes during infection. Legionella pneumophila translocates effectors via the Icm/Dot type-IV secretion system and to date, approximately 100 effectors have been identified by various experimental and computational techniques. Effector identification is a critical first step towards the understanding of the pathogenesis system in L. pneumophila as well as in other bacterial pathogens. Here, we formulate the task of effector identification as a classification problem: each L. pneumophila open reading frame (ORF) was classified as either effector or not. We computationally defined a set of features that best distinguish effectors from non-effectors. These features cover a wide range of characteristics including taxonomical dispersion, regulatory data, genomic organization, similarity to eukaryotic proteomes and more. Machine learning algorithms utilizing these features were then applied to classify all the ORFs within the L. pneumophila genome. Using this approach we were able to predict and experimentally validate 40 new effectors, reaching a success rate of above 90%. Increasing the number of validated effectors to around 140, we were able to gain novel insights into their characteristics. Effectors were found to have low G+C content, supporting the hypothesis that a large number of effectors originate via horizontal gene transfer, probably from their protozoan host. In addition, effectors were found to cluster in specific genomic regions. Finally, we were able to provide a novel description of the C-terminal translocation signal required for effector translocation by the Icm/Dot secretion system. To conclude, we have discovered 40 novel L. pneumophila effectors, predicted over a hundred additional highly probable effectors, and shown the applicability of machine learning algorithms for

  20. Genome-scale identification of Legionella pneumophila effectors using a machine learning approach.

    Directory of Open Access Journals (Sweden)

    David Burstein

    2009-07-01

    Full Text Available A large number of highly pathogenic bacteria utilize secretion systems to translocate effector proteins into host cells. Using these effectors, the bacteria subvert host cell processes during infection. Legionella pneumophila translocates effectors via the Icm/Dot type-IV secretion system and to date, approximately 100 effectors have been identified by various experimental and computational techniques. Effector identification is a critical first step towards the understanding of the pathogenesis system in L. pneumophila as well as in other bacterial pathogens. Here, we formulate the task of effector identification as a classification problem: each L. pneumophila open reading frame (ORF was classified as either effector or not. We computationally defined a set of features that best distinguish effectors from non-effectors. These features cover a wide range of characteristics including taxonomical dispersion, regulatory data, genomic organization, similarity to eukaryotic proteomes and more. Machine learning algorithms utilizing these features were then applied to classify all the ORFs within the L. pneumophila genome. Using this approach we were able to predict and experimentally validate 40 new effectors, reaching a success rate of above 90%. Increasing the number of validated effectors to around 140, we were able to gain novel insights into their characteristics. Effectors were found to have low G+C content, supporting the hypothesis that a large number of effectors originate via horizontal gene transfer, probably from their protozoan host. In addition, effectors were found to cluster in specific genomic regions. Finally, we were able to provide a novel description of the C-terminal translocation signal required for effector translocation by the Icm/Dot secretion system. To conclude, we have discovered 40 novel L. pneumophila effectors, predicted over a hundred additional highly probable effectors, and shown the applicability of machine

  1. 76 FR 21708 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-18

    ... Appendix I to OMB Circular No. A-130, ``Federal Agency Responsibilities for Maintaining Records About... Act (OSHA) reporting purposes, reports related to payment of benefits through SESA offices, State... Instruction (DoDI) 1400.25-V810, DoD Civilian Personnel Management System: Injury Compensation; DoDI 1400.25...

  2. 76 FR 79216 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-21

    ... Privacy Act of 1974; System of Records AGENCY: Federal Bureau of Prisons, Department of Justice. ACTION.... 552a), notice is given that the Federal Bureau of Prisons (Bureau) proposes to modify in part its... normal programs in the near future. The Bureau also clarifies that records may be retained at any...

  3. 75 FR 30025 - Privacy Act System of Records

    Science.gov (United States)

    2010-05-28

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  4. 78 FR 16837 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-19

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    Science.gov (United States)

    2013-06-25

    ... DEPARTMENT OF DEFENSE Department of the Army [Docket ID USA-2013-0017] Privacy Act of 1974; System... at the Defense Privacy and Civil Liberties Office Web site at http://dpclo.defense.gov/privacy/SORNs...; Army Regulation 215-3, Nonappropriated Funds Personnel Policies and Procedures; Army Regulation 215-8...

  7. 76 FR 44888 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2011-07-27

    ...'') cloud computing model. The suite is composed of Gmail for e-mail, Google Docs for office productivity... Privacy Act of 1974 (5 U.S.C. 552a), as amended, entitled ``USAID-30, Google Apps Business Edition''. This... . SUPPLEMENTARY INFORMATION: The Google Apps Business Edition is being established as an Agency-wide system of...

  8. 76 FR 13994 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-15

    ... existing inventory of record systems subject to the Privacy Act of 1974, (5 U.S.C. 552a), as amended. DATES: This proposed action will be effective without further notice on April 14, 2011 unless comments are...://www.regulations.gov . Follow the instructions for submitting comments. Mail: Federal Docket Management...

  9. 76 FR 18738 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-05

    ... Manual 7600.7-M, DoD Audit Manual; SECNAVINST 7510.7F, Department of the Navy Internal Audit and E.O... system of records are to have received Information Assurance and Privacy Act training. Computerized...: Internal Audit Policy records are maintained for 3 years then destroyed, or destroyed 1 year after guidance...

  10. 77 FR 40864 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-07-11

    ....S.C. 301, Departmental Regulations; Public Law 106-229, Electronic Signatures in Global and National Commerce Act; Presidential Directive on Electronic Commerce, July 1, 1997; OASD(C3I) Policy ] Memorandum..., retrieving, accessing, retaining, and disposing of records in the system: Storage: Electronic storage media...

  11. Regional induction of adhesion molecules and chemokine receptors explains disparate homing of human B cells to systemic and mucosal effector sites: dispersion from tonsils.

    Science.gov (United States)

    Johansen, Finn-Eirik; Baekkevold, Espen S; Carlsen, Hege S; Farstad, Inger Nina; Soler, Dulce; Brandtzaeg, Per

    2005-07-15

    Ethical constraints restrict direct tracking of immune-cell migration throughout the human body in vivo. We, therefore, used deletion of the immunoglobulin M (IgM) heavy-chain constant-gene (Cmu) segment as a marker to provide a dispersal signature of an effector B-cell subset (IgD(+)IgM(-)CD38+) induced selectively in human tonsils. By DNA analysis, the Cmu deletion identified dissemination of such blasts and their plasma-cell progeny to peripheral blood, lymph nodes, and bone marrow, as well as to mucosae and glands of the upper airways. Also the endocervix was often positive, while the small intestine was mainly negative, as could be expected from the identified homing-molecule profile of the marker cells, with relatively low levels of integrin alpha4beta7 and CC chemokine receptor 9 (CCR9). Of further importance for vaccine design, the circulating cells expressed abundantly CD62L (L-selectin) and CCR7, which provided a mechanism for integration of respiratory and systemic immunity. Most mucosal vaccines are at present administered perorally, and our results suggested that the nasal route is no alternative for vaccination against rotavirus or other small-intestinal infections in humans. However, immunization of nasopharynx-associated lymphoid tissue clearly appears preferable to target respiratory pathogens and may to some extent also protect against infections of the female genital tract.

  12. The central nervous system environment controls effector CD4+ T cell cytokine profile in experimental allergic encephalomyelitis

    DEFF Research Database (Denmark)

    Krakowski, M L; Owens, T

    1997-01-01

    In experimental allergic encephalomyelitis (EAE), CD4+ T cells infiltrate the central nervous system (CNS). We derived CD4+ T cell lines from SJL/J mice that were specific for encephalitogenic myelin basic protein (MBP) peptides and produced both Th1 and Th2 cytokines. These lines transferred EAE...

  13. AvrRxo1 Is a Bifunctional Type III Secreted Effector and Toxin-Antitoxin System Component with Homologs in Diverse Environmental Contexts.

    Directory of Open Access Journals (Sweden)

    Lindsay R Triplett

    Full Text Available Toxin-antitoxin (TA systems are ubiquitous bacterial systems that may function in genome maintenance and metabolic stress management, but are also thought to play a role in virulence by helping pathogens survive stress. We previously demonstrated that the Xanthomonas oryzae pv. oryzicola protein AvrRxo1 is a type III-secreted virulence factor that has structural similarities to the zeta family of TA toxins, and is toxic to plants and bacteria in the absence of its predicted chaperone Arc1. In this work, we confirm that AvrRxo1 and its binding partner Arc1 function as a TA system when expressed in Escherichia coli. Sequences of avrRxo1 homologs were culled from published and newly generated phytopathogen genomes, revealing that avrRxo1:arc1 modules are rare or frequently inactivated in some species and highly conserved in others. Cloning and functional analysis of avrRxo1 from Acidovorax avenae, A. citrulli, Burkholderia andropogonis, Xanthomonas translucens, and Xanthomonas euvesicatoria showed that some AvrRxo1 homologs share the bacteriostatic and Rxo1-mediated cell death triggering activities of AvrRxo1 from X. oryzae. Additional distant putative homologs of avrRxo1 and arc1 were identified in genomic or metagenomic sequence of environmental bacteria with no known pathogenic role. One of these distant homologs was cloned from the filamentous soil bacterium Cystobacter fuscus. avrRxo1 from C. fuscus caused watersoaking and triggered Rxo1-dependent cell collapse in Nicotiana benthamiana, but no growth suppression in E. coli was observed. This work confirms that a type III effector can function as a TA system toxin, and illustrates the potential of microbiome data to reveal new environmental origins or reservoirs of pathogen virulence factors.

  14. Plant-Pathogen Effectors: Cellular Probes Interfering with Plant Defenses in Spatial and Temporal Manners

    Science.gov (United States)

    Toruño, Tania Y.; Stergiopoulos, Ioannis; Coaker, Gitta

    2017-01-01

    Plants possess large arsenals of immune receptors capable of recognizing all pathogen classes. To cause disease, pathogenic organisms must be able to overcome physical barriers, suppress or evade immune perception, and derive nutrients from host tissues. Consequently, to facilitate some of these processes, pathogens secrete effector proteins that promote colonization. This review covers recent advances in the field of effector biology, focusing on conserved cellular processes targeted by effectors from diverse pathogens. The ability of effectors to facilitate pathogen entry into the host interior, suppress plant immune perception, and alter host physiology for pathogen benefit is discussed. Pathogens also deploy effectors in a spatial and temporal manner, depending on infection stage. Recent advances have also enhanced our understanding of effectors acting in specific plant organs and tissues. Effectors are excellent cellular probes that facilitate insight into biological processes as well as key points of vulnerability in plant immune signaling networks. PMID:27359369

  15. GUARANTEED LOAN SYSTEM REQUIREMENTS: Checklist for Reviewing Systems Under the Federal Financial Management Improvement Act

    National Research Council Canada - National Science Library

    2001-01-01

    ...), in Office of Management and Budget (OMB) Circular A-127, Financial Management Systems, and in OMB s Revised Implementation Guidance for the Federal Financial Management Improvement Act (FFMIA...

  16. End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology.

    Science.gov (United States)

    Dimitrijevic, Mirjana; Stanojevic, Stanislava; Kustrimovic, Natasa; Leposavic, Gordana

    2012-04-01

    Much evidence has identified a direct anatomical and functional link between the brain and the immune system, with glucocorticoids (GCs), catecholamines (CAs), and neuropeptide Y (NPY) as its end-point mediators. This suggests the important role of these mediators in immune system homeostasis and the pathogenesis of inflammatory autoimmune diseases. However, although it is clear that these mediators can modulate lymphocyte maturation and the activity of distinct immune cell types, their putative role in the pathogenesis of autoimmune disease is not yet completely understood. We have contributed to this field by discovering the influence of CAs and GCs on fine-tuning thymocyte negative selection and, in particular, by pointing to the putative CA-mediated mechanisms underlying this influence. Furthermore, we have shown that CAs are implicated in the regulation of regulatory T-cell development in the thymus. Moreover, our investigations related to macrophage biology emphasize the complex interaction between GCs, CAs and NPY in the modulation of macrophage functions and their putative significance for the pathogenesis of autoimmune inflammatory diseases.

  17. Erwinia amylovora effector protein Eop1 suppresses PAMP-triggered immunity in Malus

    Science.gov (United States)

    Erwinia amylovora (Ea) utilizes a type three secretion system (T3SS) to deliver effector proteins into plant host cells. Several Ea effectors have been identified based on their sequence similarity to plant and animal bacterial pathogen effectors; however, the function of the majority of Ea effecto...

  18. Novel Control Effectors for Truss Braced Wing

    Science.gov (United States)

    White, Edward V.; Kapania, Rakesh K.; Joshi, Shiv

    2015-01-01

    At cruise flight conditions very high aspect ratio/low sweep truss braced wings (TBW) may be subject to design requirements that distinguish them from more highly swept cantilevered wings. High aspect ratio, short chord length and relative thinness of the airfoil sections all contribute to relatively low wing torsional stiffness. This may lead to aeroelastic issues such as aileron reversal and low flutter margins. In order to counteract these issues, high aspect ratio/low sweep wings may need to carry additional high speed control effectors to operate when outboard ailerons are in reversal and/or must carry additional structural weight to enhance torsional stiffness. The novel control effector evaluated in this study is a variable sweep raked wing tip with an aileron control surface. Forward sweep of the tip allows the aileron to align closely with the torsional axis of the wing and operate in a conventional fashion. Aft sweep of the tip creates a large moment arm from the aileron to the wing torsional axis greatly enhancing aileron reversal. The novelty comes from using this enhanced and controllable aileron reversal effect to provide roll control authority by acting as a servo tab and providing roll control through intentional twist of the wing. In this case the reduced torsional stiffness of the wing becomes an advantage to be exploited. The study results show that the novel control effector concept does provide roll control as described, but only for a restricted class of TBW aircraft configurations. For the configuration studied (long range, dual aisle, Mach 0.85 cruise) the novel control effector provides significant benefits including up to 12% reduction in fuel burn.

  19. Brucella Modulates Secretory Trafficking via Multiple Type IV Secretion Effector Proteins

    Science.gov (United States)

    Myeni, Sebenzile; Child, Robert; Ng, Tony W.; Kupko, John J.; Wehrly, Tara D.; Porcella, Stephen F.; Knodler, Leigh A.; Celli, Jean

    2013-01-01

    The intracellular pathogenic bacterium Brucella generates a replicative vacuole (rBCV) derived from the endoplasmic reticulum via subversion of the host cell secretory pathway. rBCV biogenesis requires the expression of the Type IV secretion system (T4SS) VirB, which is thought to translocate effector proteins that modulate membrane trafficking along the endocytic and secretory pathways. To date, only a few T4SS substrates have been identified, whose molecular functions remain unknown. Here, we used an in silico screen to identify putative T4SS effector candidate proteins using criteria such as limited homology in other bacterial genera, the presence of features similar to known VirB T4SS effectors, GC content and presence of eukaryotic-like motifs. Using β-lactamase and CyaA adenylate cyclase reporter assays, we identified eleven proteins translocated into host cells by Brucella, five in a VirB T4SS-dependent manner, namely BAB1_0678 (BspA), BAB1_0712 (BspB), BAB1_0847 (BspC), BAB1_1671 (BspE) and BAB1_1948 (BspF). A subset of the translocated proteins targeted secretory pathway compartments when ectopically expressed in HeLa cells, and the VirB effectors BspA, BspB and BspF inhibited protein secretion. Brucella infection also impaired host protein secretion in a process requiring BspA, BspB and BspF. Single or combined deletions of bspA, bspB and bspF affected Brucella ability to replicate in macrophages and persist in the liver of infected mice. Taken together, these findings demonstrate that Brucella modulates secretory trafficking via multiple T4SS effector proteins that likely act coordinately to promote Brucella pathogenesis. PMID:23950720

  20. ROBOTIC TANK INSPECTION END EFFECTOR

    International Nuclear Information System (INIS)

    Rachel Landry

    1999-01-01

    The objective of this contract between Oceaneering Space Systems (OSS) and the Department of Energy (DOE) was to provide a tool for the DOE to inspect the inside tank walls of underground radioactive waste storage tanks in their tank farms. Some of these tanks are suspected to have leaks, but the harsh nature of the environment within the tanks precludes human inspection of tank walls. As a result of these conditions only a few inspection methods can fulfill this task. Of the methods available, OSS chose to pursue Alternating Current Field Measurement (ACFM), because it does not require clean surfaces for inspection, nor any contact with the Surface being inspected, and introduces no extra by-products in the inspection process (no coupling fluids or residues are left behind). The tool produced by OSS is the Robotic Tank Inspection End Effector (RTIEE), which is initially deployed on the tip of the Light Duty Utility Arm (LDUA). The RTEE combines ACFM with a color video camera for both electromagnetic and visual inspection The complete package consists of an end effector, its corresponding electronics and software, and a user's manual to guide the operator through an inspection. The system has both coarse and fine inspection modes and allows the user to catalog defects and suspected areas of leakage in a database for further examination, which may lead to emptying the tank for repair, decommissioning, etc.. The following is an updated report to OSS document OSS-21100-7002, which was submitted in 1995. During the course of the contract, two related sub-tasks arose, the Wall and Coating Thickness Sensor and the Vacuum Scarifying and Sampling Tool Assembly. The first of these sub-tasks was intended to evaluate the corrosion and wall thinning of 55-gallon steel drums. The second was retrieved and characterized the waste material trapped inside the annulus region of the underground tanks on the DOE's tank farms. While these sub-tasks were derived from the original intent

  1. ROBOTIC TANK INSPECTION END EFFECTOR

    Energy Technology Data Exchange (ETDEWEB)

    Rachel Landry

    1999-10-01

    The objective of this contract between Oceaneering Space Systems (OSS) and the Department of Energy (DOE) was to provide a tool for the DOE to inspect the inside tank walls of underground radioactive waste storage tanks in their tank farms. Some of these tanks are suspected to have leaks, but the harsh nature of the environment within the tanks precludes human inspection of tank walls. As a result of these conditions only a few inspection methods can fulfill this task. Of the methods available, OSS chose to pursue Alternating Current Field Measurement (ACFM), because it does not require clean surfaces for inspection, nor any contact with the Surface being inspected, and introduces no extra by-products in the inspection process (no coupling fluids or residues are left behind). The tool produced by OSS is the Robotic Tank Inspection End Effector (RTIEE), which is initially deployed on the tip of the Light Duty Utility Arm (LDUA). The RTEE combines ACFM with a color video camera for both electromagnetic and visual inspection The complete package consists of an end effector, its corresponding electronics and software, and a user's manual to guide the operator through an inspection. The system has both coarse and fine inspection modes and allows the user to catalog defects and suspected areas of leakage in a database for further examination, which may lead to emptying the tank for repair, decommissioning, etc.. The following is an updated report to OSS document OSS-21100-7002, which was submitted in 1995. During the course of the contract, two related subtasks arose, the Wall and Coating Thickness Sensor and the Vacuum Scarifying and Sampling Tool Assembly. The first of these subtasks was intended to evaluate the corrosion and wall thinning of 55-gallon steel drums. The second was retrieved and characterized the waste material trapped inside the annulus region of the underground tanks on the DOE's tank farms. While these subtasks were derived from the original

  2. Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida

    Directory of Open Access Journals (Sweden)

    Lingzhi Zhang

    2018-02-01

    Full Text Available Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Edwardsiella piscicida is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage infection, we identified 25 intracellular translocation-positive candidate effectors, including all five previously reported effectors, namely EseG, EseJ, EseH, EseK, and EvpP. A subsequent secretion analysis revealed diverse secretion patterns for the 25 effector candidates, suggesting that multiple transport pathways were involved in the internalization of these candidate effectors. Further, we identified two novel type VI secretion system (T6SS putative effectors and three outer membrane vesicles (OMV-dependent putative effectors among the candidate effectors described above, and further analyzed their contribution to bacterial virulence in a zebrafish model. This work demonstrates an effective approach for screening bacterial effectors and expands the effectors repertoire in E. piscicida.

  3. 32 CFR 806b.30 - Evaluating information systems for Privacy Act compliance.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 6 2010-07-01 2010-07-01 false Evaluating information systems for Privacy Act... systems for Privacy Act compliance. Information system owners and developers must address Privacy Act... cycle of the information system. This is accomplished with a Privacy Impact Assessment. (a) The Privacy...

  4. From bacterial avirulence genes to effector functions via the hrp delivery system: an overview of 25 years of progress in our understanding of plant innate immunity.

    Science.gov (United States)

    Mansfield, John W

    2009-11-01

    Cloning the first avirulence (avr) gene has led not only to a deeper understanding of gene-for-gene interactions in plant disease, but also to fundamental insights into the suppression of basal defences against microbial attack. This article (focusing on Pseudomonas syringae) charts the development of ideas and research progress over the 25 years following the breakthrough achieved by Staskawicz and coworkers. Advances in gene cloning technology underpinned the identification of both avr and hrp genes, the latter being required for the activation of the defensive hypersensitive reaction (HR) and pathogenicity. The delivery of Avr proteins through the type III secretion machinery encoded by hrp gene clusters was demonstrated, and the activity of the proteins inside plant cells as elicitors of the HR was confirmed. Key roles for avr genes in pathogenic fitness have now been established. The rebranding of Avr proteins as effectors, proteins that suppress the HR and cell wall-based defences, has led to the ongoing search for their targets, and is generating new insights into the co-ordination of plant resistance against diverse microbes. Bioinformatics-led analysis of effector gene distribution in genomes has provided a remarkable view of the interchange of effectors and also their functional domains, as the arms race of attack and defence drives the evolution of microbial pathogenicity. The application of our accrued knowledge for the development of disease control strategies is considered.

  5. Bartonella henselae trimeric autotransporter adhesin BadA expression interferes with effector translocation by the VirB/D4 type IV secretion system.

    Science.gov (United States)

    Lu, Yun-Yueh; Franz, Bettina; Truttmann, Matthias C; Riess, Tanja; Gay-Fraret, Jérémie; Faustmann, Marco; Kempf, Volkhard A J; Dehio, Christoph

    2013-05-01

    The Gram-negative, zoonotic pathogen Bartonella henselae is the aetiological agent of cat scratch disease, bacillary angiomatosis and peliosis hepatis in humans. Two pathogenicity factors of B. henselae - each displaying multiple functions in host cell interaction - have been characterized in greater detail: the trimeric autotransporter Bartonella adhesin A (BadA) and the type IV secretion system VirB/D4 (VirB/D4 T4SS). BadA mediates, e.g. binding to fibronectin (Fn), adherence to endothelial cells (ECs) and secretion of vascular endothelial growth factor (VEGF). VirB/D4 translocates several Bartonella effector proteins (Beps) into the cytoplasm of infected ECs, resulting, e.g. in uptake of bacterial aggregates via the invasome structure, inhibition of apoptosis and activation of a proangiogenic phenotype. Despite this knowledge of the individual activities of BadA or VirB/D4 it is unknown whether these major virulence factors affect each other in their specific activities. In this study, expression and function of BadA and VirB/D4 were analysed in a variety of clinical B. henselae isolates. Data revealed that most isolates have lost expression of either BadA or VirB/D4 during in vitro passages. However, the phenotypic effects of coexpression of both virulence factors was studied in one clinical isolate that was found to stably coexpress BadA and VirB/D4, as well as by ectopic expression of BadA in a strain expressing VirB/D4 but not BadA. BadA, which forms a dense layer on the bacterial surface, negatively affected VirB/D4-dependent Bep translocation and invasome formation by likely preventing close contact between the bacterial cell envelope and the host cell membrane. In contrast, BadA-dependent Fn binding, adhesion to ECs and VEGF secretion were not affected by a functional VirB/D4 T4SS. The obtained data imply that the essential virulence factors BadA and VirB/D4 are likely differentially expressed during different stages of the infection cycle of

  6. 76 FR 24557 - Privacy Act of 1974; as Amended; Proposed Alteration to an Existing Privacy Act System of Records...

    Science.gov (United States)

    2011-05-02

    ..., business address(es), and telephone numbers; e.g., business, fax, and cell phone. The system also will... Privacy Act System of Records, Housekeeping Changes, and New Routine Use AGENCY: Social Security Administration (SSA). ACTION: Altered system of records and housekeeping changes. SUMMARY: In accordance with the...

  7. Functions and requirements for the INEL light duty utility arm sampler end effector

    International Nuclear Information System (INIS)

    Pace, D.P.; Barnes, G.E.

    1995-02-01

    This sampler end effector system functions and requirements document defines the system functions that the end effector must perform as well as the requirements the design must meet. Safety, quality assurance, operations, environmental conditions, and regulatory requirements have been considered. The main purpose of this document is to provide a basis for the end effector engineering, design, and fabrication activities. The document shall be the living reference document to initiate the development activities and will be updated as system technologies are finalized

  8. Functions and requirements for the INEL light duty utility arm gripper end effector

    International Nuclear Information System (INIS)

    Pace, D.P.; Barnes, G.E.

    1995-02-01

    This gripper end effector system functions and requirements document defines the system functions that the end effector must perform as well as the requirements the design must meet. Safety, quality assurance, operations, environmental conditions, and regulatory requirements have been considered. The main purpose of this document is to provide a basis for the end effector engineering, design, and fabrication activities. The document shall be the living reference document to initiate the development activities and will be updated as system technologies are finalized

  9. Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors

    Science.gov (United States)

    Many bacterial pathogens of plants and animals disarm and remodel host cells by injecting large repertoires of effectors via the type III secretion system (T3SS). The repertoires of individual strains appear to function as robust systems that can tolerate loss of individual effectors with little or ...

  10. Continuously acting fuel injection system. Kontinuierlich arbeitende Kraftstoffeinspritzanlage

    Energy Technology Data Exchange (ETDEWEB)

    Dorsch, H.; Hofbauer, A.; Steinbeck, H.

    1986-02-20

    A continuously acting fuel injection system with an air quantity meter in the suction pipe and a fuel distributor controlled by it is provided with an additional device for use in a multi-cylinder aircraft engine, which is used to give the best possible performance, fuel saving and safety. An altitude control pressure controller is connected in parallel with a warming up controller to the control pressure pipe going to the fuel distributor, which measures the control pressure and therefore the fuel dosing according to the air density varying with the temperature and pressure. By fitting a fixed throttle between the control pressure pipe and the fuel return pipe to the fuel tank, emergency running of the engine is ensured on failure of both controllers. (orig./BWI).

  11. The Shigella T3SS needle transmits a signal for MxiC release, which controls secretion of effectors.

    Science.gov (United States)

    Martinez-Argudo, Isabel; Blocker, Ariel J

    2010-12-01

    Type III secretion systems (T3SSs) are key determinants of virulence in many Gram-negative bacteria, including animal and plant pathogens. They inject 'effector' proteins through a 'needle' protruding from the bacterial surface directly into eukaryotic cells after assembly of a 'translocator' pore in the host plasma membrane. Secretion is a tightly regulated process, which is blocked until physical contact with a host cell takes place. Host cell sensing occurs through a distal needle 'tip complex' and translocators are secreted before effectors. MxiC, a Shigella T3SS substrate, prevents premature effector secretion. Here, we examine how the different parts of T3SSs work together to allow orderly secretion. We show that T3SS assembly and needle tip composition are not altered in an mxiC mutant. We find that MxiC not only represses effector secretion but that it is also required for translocator release. We provide genetic evidence that MxiC acts downstream of the tip complex and then the needle during secretion activation. Finally, we show that the needle controls MxiC release. Therefore, for the first time, our data allow us to propose a model of secretion activation that goes from the tip complex to cytoplasmic MxiC via the needle. © 2010 Blackwell Publishing Ltd.

  12. Effector proteins of rust fungi.

    Science.gov (United States)

    Petre, Benjamin; Joly, David L; Duplessis, Sébastien

    2014-01-01

    Rust fungi include many species that are devastating crop pathogens. To develop resistant plants, a better understanding of rust virulence factors, or effector proteins, is needed. Thus far, only six rust effector proteins have been described: AvrP123, AvrP4, AvrL567, AvrM, RTP1, and PGTAUSPE-10-1. Although some are well established model proteins used to investigate mechanisms of immune receptor activation (avirulence activities) or entry into plant cells, how they work inside host tissues to promote fungal growth remains unknown. The genome sequences of four rust fungi (two Melampsoraceae and two Pucciniaceae) have been analyzed so far. Genome-wide analyses of these species, as well as transcriptomics performed on a broader range of rust fungi, revealed hundreds of small secreted proteins considered as rust candidate secreted effector proteins (CSEPs). The rust community now needs high-throughput approaches (effectoromics) to accelerate effector discovery/characterization and to better understand how they function in planta. However, this task is challenging due to the non-amenability of rust pathosystems (obligate biotrophs infecting crop plants) to traditional molecular genetic approaches mainly due to difficulties in culturing these species in vitro. The use of heterologous approaches should be promoted in the future.

  13. Improving a Gripper End Effector

    Energy Technology Data Exchange (ETDEWEB)

    Mullen, O Dennis; Smith, Christopher M.; Gervais, Kevin L.

    2001-01-31

    This paper discusses the improvement made to an existing four-bar linkage gripping end effector to adapt it for use in a current project. The actuating linkage was modified to yield higher jaw force overall and particularly in the critical range of jaw displacement

  14. Effectors involved in fungal-fungal interaction lead to a rare phenomenon of hyperbiotrophy in the tritrophic system biocontrol agent-powdery mildew-plant.

    Science.gov (United States)

    Laur, Joan; Ramakrishnan, Gowsica Bojarajan; Labbé, Caroline; Lefebvre, François; Spanu, Pietro D; Bélanger, Richard R

    2018-01-01

    Tritrophic interactions involving a biocontrol agent, a pathogen and a plant have been analyzed predominantly from the perspective of the biocontrol agent. We have conducted the first comprehensive transcriptomic analysis of all three organisms in an effort to understand the elusive properties of Pseudozyma flocculosa in the context of its biocontrol activity against Blumeria graminis f.sp. hordei as it parasitizes Hordeum vulgare. After inoculation of P. flocculosa, the tripartite interaction was monitored over time and samples collected for scanning electron microscopy and RNA sequencing. Based on our observations, P. flocculosa indirectly parasitizes barley, albeit transiently, by diverting nutrients extracted by B. graminis from barley leaves through a process involving unique effectors. This brings novel evidence that such molecules can also influence fungal-fungal interactions. Their release is synchronized with a higher expression of powdery mildew haustorial effectors, a sharp decline in the photosynthetic machinery of barley and a developmental peak in P. flocculosa. The interaction culminates with a collapse of B. graminis haustoria, thereby stopping P. flocculosa growth, as barley plants show higher metabolic activity. To conclude, our study has uncovered a complex and intricate phenomenon, described here as hyperbiotrophy, only achievable through the conjugated action of the three protagonists. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  15. An integrated systems approach to remote retrieval of buried transuranic waste using a telerobotic transport vehicle, innovative end effector, and remote excavator

    Energy Technology Data Exchange (ETDEWEB)

    Smith, A.M.; Rice, P.; Hyde, R. [Lockheed Idaho Technologies Co., Idaho Falls, ID (United States); Peterson, R. [RAHCO International, Spokane, WA (United States)

    1995-02-01

    Between 1952 and 1970, over two million cubic feet of transuranic mixed waste was buried in shallow pits and trenches in the Subsurface Disposal Area at the Idaho National Engineering Laboratory Radioactive Waste Management Complex. Commingled with this two million cubic feet of waste is up to 10 million cubic feet of fill soil. The pits and trenches were constructed similarly to municipal landfills with both stacked and random dump waste forms such as barrels and boxes. The main contaminants are micron-sized particles of plutonium and americium oxides, chlorides, and hydroxides. Retrieval, treatment, and disposal is one of the options being considered for the waste. This report describes the results of a field demonstration conducted to evaluate technologies for excavating, and transporting buried transuranic wastes at the INEL, and other hazardous or radioactive waste sites throughout the US Department of Energy complex. The full-scale demonstration, conduced at RAHCO Internationals facilities in Spokane, Washington, in the summer of 1994, evaluated equipment performance and techniques for digging, dumping, and transporting buried waste. Three technologies were evaluated in the demonstration: an Innovative End Effector for dust free dumping, a Telerobotic Transport Vehicle to convey retrieved waste from the digface, and a Remote Operated Excavator to deploy the Innovative End Effector and perform waste retrieval operations. Data were gathered and analyzed to evaluate retrieval performance parameters such as retrieval rates, transportation rates, human factors, and the equipment`s capability to control contamination spread.

  16. An integrated systems approach to remote retrieval of buried transuranic waste using a telerobotic transport vehicle, innovative end effector, and remote excavator

    International Nuclear Information System (INIS)

    Smith, A.M.; Rice, P.; Hyde, R.; Peterson, R.

    1995-02-01

    Between 1952 and 1970, over two million cubic feet of transuranic mixed waste was buried in shallow pits and trenches in the Subsurface Disposal Area at the Idaho National Engineering Laboratory Radioactive Waste Management Complex. Commingled with this two million cubic feet of waste is up to 10 million cubic feet of fill soil. The pits and trenches were constructed similarly to municipal landfills with both stacked and random dump waste forms such as barrels and boxes. The main contaminants are micron-sized particles of plutonium and americium oxides, chlorides, and hydroxides. Retrieval, treatment, and disposal is one of the options being considered for the waste. This report describes the results of a field demonstration conducted to evaluate technologies for excavating, and transporting buried transuranic wastes at the INEL, and other hazardous or radioactive waste sites throughout the US Department of Energy complex. The full-scale demonstration, conduced at RAHCO Internationals facilities in Spokane, Washington, in the summer of 1994, evaluated equipment performance and techniques for digging, dumping, and transporting buried waste. Three technologies were evaluated in the demonstration: an Innovative End Effector for dust free dumping, a Telerobotic Transport Vehicle to convey retrieved waste from the digface, and a Remote Operated Excavator to deploy the Innovative End Effector and perform waste retrieval operations. Data were gathered and analyzed to evaluate retrieval performance parameters such as retrieval rates, transportation rates, human factors, and the equipment's capability to control contamination spread

  17. Robotic Arm End Effector

    Science.gov (United States)

    2008-01-01

    Image illustrates the tools on the end of the arm that are used to acquire samples, image the contents of the scoop, and perform science experiments. The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  18. Three members of a peptide family are differentially distributed and elicit differential state-dependent responses in a pattern generator-effector system.

    Science.gov (United States)

    Dickinson, Patsy S; Armstrong, Matthew K; Dickinson, Evyn S; Fernandez, Rebecca; Miller, Alexandra; Pong, Sovannarath; Powers, Brian; Pupo Wiss, Alixander; Stanhope, Meredith E; Walsh, Patrick J; Wiwatpanit, Teerawat; Christie, Andrew E

    2018-01-31

    C-type allatostatins (AST-Cs) are pleiotropic neuropeptides that are broadly conserved within arthropods; the presence of three AST-C isoforms, encoded by paralog genes, is common. However, these peptides are hypothesized to act through a single receptor, thereby exerting similar bioactivities within each species. We investigated this hypothesis in the American lobster, Homarus americanus, mapping the distributions of AST-C isoforms within relevant regions of the nervous system and digestive tract, and comparing their modulatory influences on the cardiac neuromuscular system. Immunohistochemistry showed that in the pericardial organ, a neuroendocrine release site, AST-C I and/or III and AST-C II are contained within distinct populations of release terminals. Moreover, AST-C I/III-like immunoreactivity was seen in midgut epithelial endocrine cells and the cardiac ganglion (CG), whereas AST-C II-like immunoreactivity was not seen in these tissues. These data suggest that AST-C I and/or III can modulate the CG both locally and hormonally; AST-C II likely acts on the CG solely as a hormonal modulator. Physiological studies demonstrated that all three AST-C isoforms can exert differential effects, including both increases and decreases, on contraction amplitude and frequency when perfused through the heart. However, in contrast to many state-dependent modulatory changes, the changes in contraction amplitude and frequency elicited by the AST-Cs were not functions of the baseline parameters. The responses to AST-C I and III, neither of which is C-terminally amidated, are more similar to one another than they are to the responses elicited by AST-C II, which is C-terminally amidated. These results suggest that the three AST-C isoforms are differentially distributed in the lobster nervous system/midgut and can elicit distinct behaviors from the cardiac neuromuscular system, with particular structural features, e.g., C-terminal amidation, likely important in determining the

  19. 75 FR 53342 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-31

    ... and immigration information; travel; criminal and investigative history; and any other information... America by Providing Appropriate Tools Required to Intercept and Obstruct Terrorism Act of 2001 (USA...

  20. The Bordetella Secreted Regulator BspR Is Translocated into the Nucleus of Host Cells via Its N-Terminal Moiety: Evaluation of Bacterial Effector Translocation by the Escherichia coli Type III Secretion System.

    Directory of Open Access Journals (Sweden)

    Akio Abe

    Full Text Available Bordetella bronchiseptica is genetically related to B. pertussis and B. parapertussis, which cause respiratory tract infections in humans. These pathogens possess a large number of virulence factors, including the type III secretion system (T3SS, which is required for the delivery of effectors into the host cells. In a previous study, we identified a transcriptional regulator, BspR, that is involved in the regulation of the T3SS-related genes in response to iron-starved conditions. A unique feature of BspR is that this regulator is secreted into the extracellular milieu via the T3SS. To further characterize the role of BspR in extracellular localization, we constructed various truncated derivatives of BspR and investigated their translocation into the host cells using conventional translocation assays. In this study, the effector translocation was evaluated by the T3SS of enteropathogenic E. coli (EPEC, since the exogenous expression of BspR triggers severe repression of the Bordetella T3SS expression. The results of the translocation assays using the EPEC T3SS showed that the N-terminal 150 amino acid (aa residues of BspR are sufficient for translocation into the host cells in a T3SS-dependent manner. In addition, exogenous expression of BspR in HeLa cells demonstrated that the N-terminal 100 aa residues are involved in the nuclear localization. In contrast, the N-terminal 54 aa residues are sufficient for the extracellular secretion into the bacterial culture supernatant via the EPEC T3SS. Thus, BspR is not only a transcriptional regulator in bacteria cytosol, but also functions as an effector that translocates into the nuclei of infected host cells.

  1. Difference of Type 3 secretion system (T3SS) effector gene genotypes (exoU and exoS) and its implication to antibiotics resistances in isolates of Pseudomonas aeruginosa from chronic otitis media.

    Science.gov (United States)

    Park, Min-Hyun; Kim, So Young; Roh, Eun Yun; Lee, Ho Sun

    2017-06-01

    Type 3 secretion system (T3SS) is the most important virulence factor in Pseudomonas aeruginosa infection. Of the various T3SS effector genes, exoS and exoU showed mutually exclusive distributions, and these two genes showed varied virulence. In many pseudomonal infections, the distribution of these genes showed different pattern and it influenced severity of infection. This study was aimed to evaluate differences of virulence factors and antibiotics resistance between chronic otitis media and other body infection caused by P. aeruginosa. To estimate the prevalence of effector genes of T3SS, especially the distributions of exoS and exoU genes and their association with antibiotic resistance in COM, we compared the prevalence of T3SS genes in isolates from COM with those from lower respiratory infection and bacteremia. Other virulence genes, including groEL, pilA, ndvB, lasB, rhlI, and apr, were also studied to evaluate prevalence. These isolates were tested for antibiotic susceptibility, and we examined the association between antibiotic susceptibility and the prevalence of T3SS effector genes. The COM group showed a significantly higher exoU-positive rate than the control group (70.6% vs. 6.7%; P<0.01). Furthermore, COM patients with exoU showed significant antibiotic resistance to ciprofloxacin and tobramycin (P=0.035), whereas there was no significant difference in the control group. The high incidence of exoU-positive P. aeruginosa and ciprofloxacin resistance can explain the chronicity and intractability of infection in COM. Elucidation of this pathogenicity will facilitate the development of new treatment options for COM patients. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. The Bordetella Secreted Regulator BspR Is Translocated into the Nucleus of Host Cells via Its N-Terminal Moiety: Evaluation of Bacterial Effector Translocation by the Escherichia coli Type III Secretion System.

    Science.gov (United States)

    Abe, Akio; Nishimura, Ryutaro; Tanaka, Naomichi; Kurushima, Jun; Kuwae, Asaomi

    2015-01-01

    Bordetella bronchiseptica is genetically related to B. pertussis and B. parapertussis, which cause respiratory tract infections in humans. These pathogens possess a large number of virulence factors, including the type III secretion system (T3SS), which is required for the delivery of effectors into the host cells. In a previous study, we identified a transcriptional regulator, BspR, that is involved in the regulation of the T3SS-related genes in response to iron-starved conditions. A unique feature of BspR is that this regulator is secreted into the extracellular milieu via the T3SS. To further characterize the role of BspR in extracellular localization, we constructed various truncated derivatives of BspR and investigated their translocation into the host cells using conventional translocation assays. In this study, the effector translocation was evaluated by the T3SS of enteropathogenic E. coli (EPEC), since the exogenous expression of BspR triggers severe repression of the Bordetella T3SS expression. The results of the translocation assays using the EPEC T3SS showed that the N-terminal 150 amino acid (aa) residues of BspR are sufficient for translocation into the host cells in a T3SS-dependent manner. In addition, exogenous expression of BspR in HeLa cells demonstrated that the N-terminal 100 aa residues are involved in the nuclear localization. In contrast, the N-terminal 54 aa residues are sufficient for the extracellular secretion into the bacterial culture supernatant via the EPEC T3SS. Thus, BspR is not only a transcriptional regulator in bacteria cytosol, but also functions as an effector that translocates into the nuclei of infected host cells.

  3. Principles and applications of TAL effectors for plant physiology and metabolism.

    Science.gov (United States)

    Bogdanove, Adam J

    2014-06-01

    Recent advances in DNA targeting allow unprecedented control over gene function and expression. Targeting based on TAL effectors is arguably the most promising for systems biology and metabolic engineering. Multiple, orthogonal TAL-effector reagents of different types can be used in the same cell. Furthermore, variation in base preferences of the individual structural repeats that make up the TAL effector DNA recognition domain makes targeting stringency tunable. Realized applications range from genome editing to epigenome modification to targeted gene regulation to chromatin labeling and capture. The principles that govern TAL effector DNA recognition make TAL effectors well suited for applications relevant to plant physiology and metabolism. TAL effector targeting has merits that are distinct from those of the RNA-based DNA targeting CRISPR/Cas9 system. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. 76 FR 77498 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-13

    ..., Health Insurance Portability and Accountability Act of 1996; and E.O. 9397 (SSN), as amended... governing local control of communicable diseases, preventive medicine and safety programs, child abuse and... to the Health Insurance Portability and Accountability Act of 1996, applies to most such health...

  5. In Planta Functional Analysis and Subcellular Localization of the Oomycete Pathogen Plasmopara viticola Candidate RXLR Effector Repertoire

    Directory of Open Access Journals (Sweden)

    Yunxiao Liu

    2018-04-01

    Full Text Available Downy mildew is one of the most destructive diseases of grapevine, causing tremendous economic loss in the grape and wine industry. The disease agent Plasmopara viticola is an obligate biotrophic oomycete, from which over 100 candidate RXLR effectors have been identified. In this study, 83 candidate RXLR effector genes (PvRXLRs were cloned from the P. viticola isolate “JL-7-2” genome. The results of the yeast signal sequence trap assay indicated that most of the candidate effectors are secretory proteins. The biological activities and subcellular localizations of all the 83 effectors were analyzed via a heterologous Agrobacterium-mediated Nicotiana benthamiana expression system. Results showed that 52 effectors could completely suppress cell death triggered by elicitin, 10 effectors could partially suppress cell death, 11 effectors were unable to suppress cell death, and 10 effectors themselves triggered cell death. Live-cell imaging showed that the majority of the effectors (76 of 83 could be observed with informative fluorescence signals in plant cells, among which 34 effectors were found to be targeted to both the nucleus and cytosol, 29 effectors were specifically localized in the nucleus, and 9 effectors were targeted to plant membrane system. Interestingly, three effectors PvRXLR61, 86 and 161 were targeted to chloroplasts, and one effector PvRXLR54 was dually targeted to chloroplasts and mitochondria. However, western blot analysis suggested that only PvRXLR86 carried a cleavable N-terminal transit peptide and underwent processing in planta. Many effectors have previously been predicted to target organelles, however, to the best of our knowledge, this is the first study to provide experimental evidence of oomycete effectors targeted to chloroplasts and mitochondria.

  6. 77 FR 65245 - Privacy Act; System of Records: Visa Records, State-39

    Science.gov (United States)

    2012-10-25

    ... DEPARTMENT OF STATE [Public Notice 8070] Privacy Act; System of Records: Visa Records, State-39... records, Visa Records, State-39, pursuant to the provisions of the Privacy Act of 1974, as amended (5 U.S... of records, Visa Records, State-39, to ensure Privacy Act of 1974 compliance: Purpose. The Department...

  7. The Danish patient safety experience: the Act on Patient Safety in the Danish Health care system

    DEFF Research Database (Denmark)

    Lundgaard, Mette; Rabøl, Louise; Jensen, Elisabeth Agnete Brøgger

    2005-01-01

    This paper describes the process that lead to the passing of the Act for Patient Safety in the Danisk health care sytem, the contents of the act and how the act is used in the Danish health care system. The act obligates frontline health care personnel to report adverse events, hospital owners...... to act on the reports and the National Board of Health to commuicate the learning nationally. The act protects health care providers from sanctions as a result of reporting. In January 2004, the Act on Patient Safety in the Danish health care system was put into force. In the first twelve months 5740...... adverse events were reported. the reports were analyzed locally (hospital and region), anonymized ad then sent to the National Board af Health. The Act on Patient Safety has driven the work with patient safety forward but there is room for improvement. Continuous and improved feedback from all parts...

  8. The Danish patient safety experience: the Act on Patient Safety in the Danish Health care system

    DEFF Research Database (Denmark)

    Lundgaard, Mette; Rabøl, Louise; Jensen, Elisabeth Agnete Brøgger

    2005-01-01

    adverse events were reported. the reports were analyzed locally (hospital and region), anonymized ad then sent to the National Board af Health. The Act on Patient Safety has driven the work with patient safety forward but there is room for improvement. Continuous and improved feedback from all parts......This paper describes the process that lead to the passing of the Act for Patient Safety in the Danisk health care sytem, the contents of the act and how the act is used in the Danish health care system. The act obligates frontline health care personnel to report adverse events, hospital owners...... to act on the reports and the National Board of Health to commuicate the learning nationally. The act protects health care providers from sanctions as a result of reporting. In January 2004, the Act on Patient Safety in the Danish health care system was put into force. In the first twelve months 5740...

  9. 75 FR 65007 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-21

    ... Officer, P.O. Box 2218, Waldorf, MD 20604-2218. Individuals should complete AFOSI's Certification of... of Information/Privacy Act Officer, P.O. Box 2218, Waldorf, MD 20604-2218. Individuals should...

  10. 78 FR 25414 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2013-05-01

    ... Reinvestigating Individuals in Positions of Public Trust; and the Intelligence Reform and Terrorism Prevention Act... controlled by electronic card readers, office personnel to control access, visitor escorts policy and...

  11. 78 FR 41962 - Privacy Act of 1974: Systems of Records

    Science.gov (United States)

    2013-07-12

    ... Information Act (FOIA), and to facilitate OGIS' offering of mediation services to resolve disputes between... work of the Commission or conducting records management inspections. 6. To a commercial contractor in...

  12. 76 FR 53421 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-26

    ..., Air Force Privacy Act Office, Office of Warfighting Integration and Chief Information Officer, ATTN...: Name, signatures, personal contact information, individual's employer and institutional, organizational..., Historical Products, Services, and Requirements; Air Force Instruction 84-105, Organizational Lineage, Honors...

  13. 76 FR 35052 - Privacy Act of 1974: New System of Records

    Science.gov (United States)

    2011-06-15

    ... MANAGEMENT Privacy Act of 1974: New System of Records AGENCY: U.S. Office of Personnel Management. ACTION: Notice of a new system of records. SUMMARY: The U.S. Office of Personnel Management (OPM), Office of the... Data Warehouse to its inventory of records systems subject to the Privacy Act of 1974 (5 U.S.C. 552a...

  14. 75 FR 47812 - Privacy Act of 1974; Report of a New System of Records

    Science.gov (United States)

    2010-08-09

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Office of Security and Strategic Information Privacy Act... Strategic Information. SYSTEM NO. 09-90-0777 SYSTEM NAME: ``Facility and Resource Access Control Records... system user name; user access and [[Page 47815

  15. Special-purpose multifingered robotic end-effectors

    International Nuclear Information System (INIS)

    Crowder, R.M.

    1990-01-01

    A number of advanced multifingered robotic end-effectors have been developed recently in which the finger joints are powered from external actuators. Although this gives dexterous performance, there are considerable problems with power transmission, due to the use of flexible tendons between the external actuators and the individual finger joints. If a multifingered robotic end-effector is to be operated in a confined space, local actuation of the fingers needs to be fully considered, even if there is a reduction in hand dexterity over that of an externally mounted actuator system. The University of Southampton has developed a number of end-effectors that incorporate integral finger actuators and mechanisms, two examples of which are discussed in this paper

  16. 77 FR 56676 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-13

    ... LOCATION: [CHANGE TO READ] Postmaster General, Government Relations, and Consumer and Industry Affairs... the FOIA or the Privacy Act, or who request amendment of a record, including name, Social Security... burning, pulping, or shredding. Records existing on computer storage media are destroyed according to the...

  17. 75 FR 59242 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-09-27

    ... title IV, HEA programs; (2) to store electronic data that support the existence of a legal obligation to... complex password policy. In addition to the enforcement of the complex password policy, users are required... programs authorized by title IV of the Higher Education Act of 1965, as amended (HEA), and to include...

  18. 75 FR 32915 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-10

    ... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID: DOD-2010-OS-0076] Privacy Act of 1974... installation access control and random patrols. Common Access Cards and personal identification numbers are... name, Social Security Number (SSN), student identification number, date of birth, e-mail, home...

  19. 75 FR 61135 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-04

    ... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID: DOD-2010-OS-0138] Privacy Act of 1974... access, or monitored areas. Database is monitored and access is password protected and Common Access Card... or universities funded by DoD or other U.S. Federal Government agencies; students and employees of...

  20. 78 FR 77719 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2013-12-24

    ... employment such as name, birth date, Social Security Number, home contact information, marital status... electronic media and/or as hard-copy documents. RETRIEVABILITY: All records are retrieved from the system by.... SYSTEM MANAGERS AND ADDRESSES: Contractor Industrial Relations Officer, Location 1. Subsystem Managers...

  1. Yersinia type III effectors perturb host innate immune responses

    Science.gov (United States)

    Pha, Khavong; Navarro, Lorena

    2016-01-01

    The innate immune system is the first line of defense against invading pathogens. Innate immune cells recognize molecular patterns from the pathogen and mount a response to resolve the infection. The production of proinflammatory cytokines and reactive oxygen species, phagocytosis, and induced programmed cell death are processes initiated by innate immune cells in order to combat invading pathogens. However, pathogens have evolved various virulence mechanisms to subvert these responses. One strategy utilized by Gram-negative bacterial pathogens is the deployment of a complex machine termed the type III secretion system (T3SS). The T3SS is composed of a syringe-like needle structure and the effector proteins that are injected directly into a target host cell to disrupt a cellular response. The three human pathogenic Yersinia spp. (Y. pestis, Y. enterocolitica, and Y. pseudotuberculosis) are Gram-negative bacteria that share in common a 70 kb virulence plasmid which encodes the T3SS. Translocation of the Yersinia effector proteins (YopE, YopH, YopT, YopM, YpkA/YopO, and YopP/J) into the target host cell results in disruption of the actin cytoskeleton to inhibit phagocytosis, downregulation of proinflammatory cytokine/chemokine production, and induction of cellular apoptosis of the target cell. Over the past 25 years, studies on the Yersinia effector proteins have unveiled tremendous knowledge of how the effectors enhance Yersinia virulence. Recently, the long awaited crystal structure of YpkA has been solved providing further insights into the activation of the YpkA kinase domain. Multisite autophosphorylation by YpkA to activate its kinase domain was also shown and postulated to serve as a mechanism to bypass regulation by host phosphatases. In addition, novel Yersinia effector protein targets, such as caspase-1, and signaling pathways including activation of the inflammasome were identified. In this review, we summarize the recent discoveries made on Yersinia

  2. 75 FR 61450 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-05

    ...; System of Records AGENCY: Defense Finance and Accounting Service; DoD. ACTION: Notice to add a system of records. SUMMARY: The Defense Finance and Accounting Service (DFAS) is proposing to add a system of... System Name: Standard Finance System (STANFINS). System Location: Defense Information Systems Agency...

  3. Space Station end effector strategy study

    Science.gov (United States)

    Katzberg, Stephen J.; Jensen, Robert L.; Willshire, Kelli F.; Satterthwaite, Robert E.

    1987-01-01

    The results of a study are presented for terminology definition, identification of functional requirements, technolgy assessment, and proposed end effector development strategies for the Space Station Program. The study is composed of a survey of available or under-developed end effector technology, identification of requirements from baselined Space Station documents, a comparative assessment of the match between technology and requirements, and recommended strategies for end effector development for the Space Station Program.

  4. Identification of proteins similar to AvrE type III effector proteins from ...

    African Journals Online (AJOL)

    Type III effector proteins are injected into host cells through type III secretion systems. Some effectors are similar to host proteins to promote pathogenicity, while others lead to the activation of disease resistance. We used partial least squares alignment-free bioinformatics methods to identify proteins similar to AvrE proteins ...

  5. The enteropathogenic Escherichia coli (EPEC) Map effector is imported into the mitochondrial matrix by the TOM/Hsp70 system and alters organelle morphology.

    Science.gov (United States)

    Papatheodorou, Panagiotis; Domańska, Grazyna; Oxle, Marius; Mathieu, Johannes; Selchow, Olaf; Kenny, Brendan; Rassow, Joachim

    2006-04-01

    Enteropathogenic Escherichia coli (EPEC) is a human intestinal pathogen and a major cause of diarrhoea, particularly among infants in developing countries. EPEC target the Map and EspF multifunctional effector proteins to host mitochondria - organelles that play crucial roles in regulating cellular processes such as programmed cell death (apoptosis). While both molecules interfere with the organelles ability to maintain a membrane potential, EspF plays the predominant role and is responsible for triggering cell death. To learn more about the Map-mitochondria interaction, we studied Map localization to mitochondria with purified mitochondria (from mammalian and yeast cells) and within intact yeast. This revealed that (i) Map targeting is dependent on the predicted N-terminal mitochondrial targeting sequence, (ii) the N-terminal 44 residues are sufficient to target proteins to mitochondria and (iii) Map import involves the mitochondrial outer membrane translocase (Tom22 and Tom40), the mitochondrial membrane potential, and the matrix chaperone, mtHsp70. These results are consistent with Map import into the mitochondria matrix via the classical import mechanism. As all known, Map-associated phenotypes in mammalian cells are independent of mitochondrial targeting, this may indicate that import serves as a mechanism to remove Map from the cytoplasm thereby regulating cytoplasmic function. Intriguingly, Map, but not EspF, alters mitochondrial morphology with deletion analysis revealing important roles for residues 101-152. Changes in mitochondrial morphology have been linked to alterations in the ability of these organelles to regulate cellular processes providing a possible additional role for Map import into mitochondria.

  6. 77 FR 24929 - Privacy Act of 1974; New System of Records

    Science.gov (United States)

    2012-04-26

    ... Survey Database. II. Privacy Act The Privacy Act embodies fair information principles in a statutory.... The results from the survey are stored in a separate database and are not a part of this system of... of the USDA/FS-58 National Woodland Owner Survey Database. A report of the proposed system of records...

  7. 75 FR 75546 - Financial Management Service; Privacy Act of 1974, as Amended; System of Records

    Science.gov (United States)

    2010-12-03

    ... DEPARTMENT OF THE TREASURY Financial Management Service; Privacy Act of 1974, as Amended; System of Records AGENCY: Financial Management Service, Treasury. ACTION: Notice of proposed new system of records. SUMMARY: In accordance with the Privacy Act of 1974, as amended, the Financial Management Service...

  8. 77 FR 5062 - Privacy Act of 1974; Consolidation of National Labor Relations Board Systems of Records

    Science.gov (United States)

    2012-02-01

    ... BOARD Privacy Act of 1974; Consolidation of National Labor Relations Board Systems of Records AGENCY: National Labor Relations Board. ACTION: Notification of the consolidation of six Privacy Act systems of... accessible, but no additional, unique information is being added to them. At this time, information described...

  9. Using hierarchical clustering of secreted protein families to classify and rank candidate effectors of rust fungi.

    Science.gov (United States)

    Saunders, Diane G O; Win, Joe; Cano, Liliana M; Szabo, Les J; Kamoun, Sophien; Raffaele, Sylvain

    2012-01-01

    Rust fungi are obligate biotrophic pathogens that cause considerable damage on crop plants. Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, and Melampsora larici-populina, the poplar leaf rust pathogen, have strong deleterious impacts on wheat and poplar wood production, respectively. Filamentous pathogens such as rust fungi secrete molecules called disease effectors that act as modulators of host cell physiology and can suppress or trigger host immunity. Current knowledge on effectors from other filamentous plant pathogens can be exploited for the characterisation of effectors in the genome of recently sequenced rust fungi. We designed a comprehensive in silico analysis pipeline to identify the putative effector repertoire from the genome of two plant pathogenic rust fungi. The pipeline is based on the observation that known effector proteins from filamentous pathogens have at least one of the following properties: (i) contain a secretion signal, (ii) are encoded by in planta induced genes, (iii) have similarity to haustorial proteins, (iv) are small and cysteine rich, (v) contain a known effector motif or a nuclear localization signal, (vi) are encoded by genes with long intergenic regions, (vii) contain internal repeats, and (viii) do not contain PFAM domains, except those associated with pathogenicity. We used Markov clustering and hierarchical clustering to classify protein families of rust pathogens and rank them according to their likelihood of being effectors. Using this approach, we identified eight families of candidate effectors that we consider of high value for functional characterization. This study revealed a diverse set of candidate effectors, including families of haustorial expressed secreted proteins and small cysteine-rich proteins. This comprehensive classification of candidate effectors from these devastating rust pathogens is an initial step towards probing plant germplasm for novel resistance components.

  10. Using hierarchical clustering of secreted protein families to classify and rank candidate effectors of rust fungi.

    Directory of Open Access Journals (Sweden)

    Diane G O Saunders

    Full Text Available Rust fungi are obligate biotrophic pathogens that cause considerable damage on crop plants. Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, and Melampsora larici-populina, the poplar leaf rust pathogen, have strong deleterious impacts on wheat and poplar wood production, respectively. Filamentous pathogens such as rust fungi secrete molecules called disease effectors that act as modulators of host cell physiology and can suppress or trigger host immunity. Current knowledge on effectors from other filamentous plant pathogens can be exploited for the characterisation of effectors in the genome of recently sequenced rust fungi. We designed a comprehensive in silico analysis pipeline to identify the putative effector repertoire from the genome of two plant pathogenic rust fungi. The pipeline is based on the observation that known effector proteins from filamentous pathogens have at least one of the following properties: (i contain a secretion signal, (ii are encoded by in planta induced genes, (iii have similarity to haustorial proteins, (iv are small and cysteine rich, (v contain a known effector motif or a nuclear localization signal, (vi are encoded by genes with long intergenic regions, (vii contain internal repeats, and (viii do not contain PFAM domains, except those associated with pathogenicity. We used Markov clustering and hierarchical clustering to classify protein families of rust pathogens and rank them according to their likelihood of being effectors. Using this approach, we identified eight families of candidate effectors that we consider of high value for functional characterization. This study revealed a diverse set of candidate effectors, including families of haustorial expressed secreted proteins and small cysteine-rich proteins. This comprehensive classification of candidate effectors from these devastating rust pathogens is an initial step towards probing plant germplasm for novel resistance components.

  11. The genome sequence and effector complement of the flax rust pathogen Melampsora lini

    Directory of Open Access Journals (Sweden)

    Adnane eNemri

    2014-03-01

    Full Text Available Rust fungi cause serious yield reductions on crops, including wheat, barley, soybean, coffee, and represent real threats to global food security. Of these fungi, the flax rust pathogen Melampsora lini has been developed extensively over the past 80 years as a model to understand the molecular mechanisms that underpin pathogenesis. During infection, M. lini secretes virulence effectors to promote disease. The number of these effectors, their function and their degree of conservation across rust fungal species is unknown. To assess this, we sequenced and assembled de novo the genome of M. lini isolate CH5 into 21,130 scaffolds spanning 189 Mbp (scaffold N50 of 31 kbp. Global analysis of the DNA sequence revealed that repetitive elements, primarily retrotransposons, make up at least 45% of the genome. Using ab initio predictions, transcriptome data and homology searches, we identified 16,271 putative protein-coding genes. An analysis pipeline was then implemented to predict the effector complement of M. lini and compare it to that of the poplar rust, wheat stem rust and wheat stripe rust pathogens to identify conserved and species-specific effector candidates. Previous knowledge of four cloned M. lini avirulence effector proteins and two basidiomycete effectors was used to optimise parameters of the effector prediction pipeline. Markov clustering based on sequence similarity was performed to group effector candidates from all four rust pathogens. Clusters containing at least one member from M. lini were further analysed and prioritized based on features including expression in isolated haustoria and infected leaf tissue and conservation across rust species. Herein, we describe 200 of 940 clusters that ranked highest on our priority list, representing 725 flax rust candidate effectors. Our findings on this important model rust species provide insight into how effectors of rust fungi are conserved across species and how they may act to promote

  12. Using Hierarchical Clustering of Secreted Protein Families to Classify and Rank Candidate Effectors of Rust Fungi

    Science.gov (United States)

    Saunders, Diane G. O.; Win, Joe; Cano, Liliana M.; Szabo, Les J.; Kamoun, Sophien; Raffaele, Sylvain

    2012-01-01

    Rust fungi are obligate biotrophic pathogens that cause considerable damage on crop plants. Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, and Melampsora larici-populina, the poplar leaf rust pathogen, have strong deleterious impacts on wheat and poplar wood production, respectively. Filamentous pathogens such as rust fungi secrete molecules called disease effectors that act as modulators of host cell physiology and can suppress or trigger host immunity. Current knowledge on effectors from other filamentous plant pathogens can be exploited for the characterisation of effectors in the genome of recently sequenced rust fungi. We designed a comprehensive in silico analysis pipeline to identify the putative effector repertoire from the genome of two plant pathogenic rust fungi. The pipeline is based on the observation that known effector proteins from filamentous pathogens have at least one of the following properties: (i) contain a secretion signal, (ii) are encoded by in planta induced genes, (iii) have similarity to haustorial proteins, (iv) are small and cysteine rich, (v) contain a known effector motif or a nuclear localization signal, (vi) are encoded by genes with long intergenic regions, (vii) contain internal repeats, and (viii) do not contain PFAM domains, except those associated with pathogenicity. We used Markov clustering and hierarchical clustering to classify protein families of rust pathogens and rank them according to their likelihood of being effectors. Using this approach, we identified eight families of candidate effectors that we consider of high value for functional characterization. This study revealed a diverse set of candidate effectors, including families of haustorial expressed secreted proteins and small cysteine-rich proteins. This comprehensive classification of candidate effectors from these devastating rust pathogens is an initial step towards probing plant germplasm for novel resistance components. PMID:22238666

  13. The genome sequence and effector complement of the flax rust pathogen Melampsora lini.

    Science.gov (United States)

    Nemri, Adnane; Saunders, Diane G O; Anderson, Claire; Upadhyaya, Narayana M; Win, Joe; Lawrence, Gregory J; Jones, David A; Kamoun, Sophien; Ellis, Jeffrey G; Dodds, Peter N

    2014-01-01

    Rust fungi cause serious yield reductions on crops, including wheat, barley, soybean, coffee, and represent real threats to global food security. Of these fungi, the flax rust pathogen Melampsora lini has been developed most extensively over the past 80 years as a model to understand the molecular mechanisms that underpin pathogenesis. During infection, M. lini secretes virulence effectors to promote disease. The number of these effectors, their function and their degree of conservation across rust fungal species is unknown. To assess this, we sequenced and assembled de novo the genome of M. lini isolate CH5 into 21,130 scaffolds spanning 189 Mbp (scaffold N50 of 31 kbp). Global analysis of the DNA sequence revealed that repetitive elements, primarily retrotransposons, make up at least 45% of the genome. Using ab initio predictions, transcriptome data and homology searches, we identified 16,271 putative protein-coding genes. An analysis pipeline was then implemented to predict the effector complement of M. lini and compare it to that of the poplar rust, wheat stem rust and wheat stripe rust pathogens to identify conserved and species-specific effector candidates. Previous knowledge of four cloned M. lini avirulence effector proteins and two basidiomycete effectors was used to optimize parameters of the effector prediction pipeline. Markov clustering based on sequence similarity was performed to group effector candidates from all four rust pathogens. Clusters containing at least one member from M. lini were further analyzed and prioritized based on features including expression in isolated haustoria and infected leaf tissue and conservation across rust species. Herein, we describe 200 of 940 clusters that ranked highest on our priority list, representing 725 flax rust candidate effectors. Our findings on this important model rust species provide insight into how effectors of rust fungi are conserved across species and how they may act to promote infection on their

  14. 77 FR 61467 - Privacy Act of 1974: Revision of Privacy Act System of Records

    Science.gov (United States)

    2012-10-09

    ... being made, and to identify and mitigate, fraud, waste and abuse. SBA System 36--Suspension and...) a fiscal or financial agent designated by the Department of the Treasury, including employees...

  15. 77 FR 15086 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-03-14

    ... Defense Intelligence Information System (DoDIIS) Customer Relationship Management System. The records will... instructions for submitting comments. * Mail: Federal Docket Management System Office, 4800 Mark Center Drive...; System of Records AGENCY: Defense Intelligence Agency, DoD. [[Page 15087

  16. 78 FR 70543 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-26

    ...; System of Records AGENCY: Defense Information Systems Agency, DoD. ACTION: Notice to add a new system of records. SUMMARY: The Defense Information Systems Agency proposes to add a new system of records, KWHC 08..., and appraisal throughout the lifecycle. This system is used to track travel, security, sensitive items...

  17. 76 FR 13671 - Privacy Act of 1974, as Amended; System of Records Notices

    Science.gov (United States)

    2011-03-14

    ... United States Code permits the Archivist of the United States to maintain, operate, and protect land..., 2011. David S. Ferriero, Archivist of the United States. NARA Privacy Act Systems: NARA 41 SYSTEM NAME...

  18. 76 FR 62035 - Privacy Act of 1974: Notice of Proposed Privacy Act System of Records Revision

    Science.gov (United States)

    2011-10-06

    .... Portable nuclear gauges are used to make water measurements in soil and for road and dam construction... programs and for other purposes. Electron capture detectors are used as a component of gas chromatographs... years. Lab Surveys: 3 years. Leak Tests: 5 years. SYSTEM MANAGER(S) AND ADDRESS: The mailing address for...

  19. 77 FR 15835 - Privacy Act of 1974: Revision of Privacy Act System of Records

    Science.gov (United States)

    2012-03-16

    ... Credit Risk Management; Director, Office of Financial Assistance; Regional Administrators; District... Counsel Division 409 Third Street SW., Washington, DC 20416. Office of Inspector General Management... agents). CATEGORIES OF RECORDS IN THE SYSTEM INCLUDE: Personal and commercial information (i.e., credit...

  20. 76 FR 78050 - Privacy Act of 1974; Privacy Act System of Records

    Science.gov (United States)

    2011-12-15

    ... be involved in cooperation with NASA under international agreements; foreign media representatives... include high-level protocol visits; or international relations. CATEGORIES OF RECORDS IN THE SYSTEM... include, but are not limited to: Name, former names, date of birth, place of birth, social security number...

  1. 78 FR 69076 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-18

    ... Defense (DoD), systems of records are grouped by the DoD Component responsible for that system and its system of records notice. Each DoD Component has an assigned letter denominator for systems of records.... DHA 07 EDHA 07 Military Health Information System. DHA 08 EDHA 08 Health Affairs Survey and Study Data...

  2. 75 FR 2114 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-01-14

    ...; Systems of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to alter a system of records. SUMMARY: The Defense Finance and Accounting Service (DFAS) is proposing to alter a system... Defense Finance and Accounting Service compilation of systems of records notices also apply to this system...

  3. 77 FR 65370 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-26

    ... respective personnel management and finance systems.'' Routine uses of records maintained in the system...; System of Records AGENCY: Defense Logistics Agency, DoD. ACTION: Notice to Alter a System of Records. SUMMARY: The Defense Logistics Agency proposes to alter a system of records in its inventory of record...

  4. 78 FR 14276 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Management Information System Records, NM01560-2 Department of Defense Voluntary Education System, and...; NM01560-2 Department of Defense Voluntary Education System, and NM01500-2 Department of the Navy Education...; System of Records AGENCY: United States Marine Corps, DoD. ACTION: Notice to delete twenty-three Systems...

  5. 75 FR 81247 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-12-27

    ... Control Records system of records is also covered by the Defense Finance and Accounting System T7330a... Defense Finance and Accounting System T7332, Defense Debt Management System (February 17, 2009, 74 FR 7665... instructions for submitting comments. Mail: Federal Docket Management System Office, Room 3C843, 1160 Defense...

  6. 77 FR 22290 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-04-13

    ... of Defense Intelligence Information System (DoDIIS) Customer Relationship Management.'' System.... * Mail: Federal Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd Floor, Suite...; System of Records AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice to alter a system of records...

  7. 76 FR 72909 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-28

    ... Telephone Use Records (August 7, 2009, 74 FR 39652). Changes: * * * * * System location: Delete entry and....'' * * * * * S284.89 System name: Government Telephone Use Records. System location: Records are located at System... members, contractors, and individuals authorized to use government telephone systems, including wireless...

  8. Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of Xanthomonas oryzae pv. oryzae.

    Directory of Open Access Journals (Sweden)

    Dipanwita Sinha

    Full Text Available Innate immune responses are induced in plants and animals through perception of Damage Associated Molecular Patterns. These immune responses are suppressed by pathogens during infection. A number of studies have focussed on identifying functions of plant pathogenic bacteria that are involved in suppression of Pathogen Associated Molecular Pattern induced immune responses. In comparison, there is very little information on functions used by plant pathogens to suppress Damage Associated Molecular Pattern induced immune responses. Xanthomonasoryzae pv. oryzae, a gram negative bacterial pathogen of rice, secretes hydrolytic enzymes such as LipA (Lipase/Esterase that damage rice cell walls and induce innate immune responses. Here, we show that Agrobacterium mediated transient transfer of the gene for XopN, a X. oryzae pv. oryzae type 3 secretion (T3S system effector, results in suppression of rice innate immune responses induced by LipA. A xopN (- mutant of X. oryzae pv. oryzae retains the ability to suppress these innate immune responses indicating the presence of other functionally redundant proteins. In transient transfer assays, we have assessed the ability of 15 other X. oryzae pv. oryzae T3S secreted effectors to suppress rice innate immune responses. Amongst these proteins, XopQ, XopX and XopZ are suppressors of LipA induced innate immune responses. A mutation in any one of the xopN, xopQ, xopX or xopZ genes causes partial virulence deficiency while a xopN (- xopX (- double mutant exhibits a greater virulence deficiency. A xopN (- xopQ (- xopX (- xopZ (- quadruple mutant of X. oryzae pv. oryzae induces callose deposition, an innate immune response, similar to a X. oryzae pv. oryzae T3S(- mutant in rice leaves. Overall, these results indicate that multiple T3S secreted proteins of X. oryzae pv. oryzae can suppress cell wall damage induced rice innate immune responses.

  9. 77 FR 24925 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-04-26

    ... system: Categories of records in this system include: Personnel and payroll information on professional... and in a computerized system at the National Institute of Food and Agriculture (NIFA), Department of...

  10. 77 FR 11534 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2012-02-27

    ... SYSTEM NAME: EEO Discrimination Complaint File. FRTIB-4 SYSTEM NAME: Adverse Information and Action... NAME: EEO Discrimination Complaint File. SYSTEM LOCATION: Federal Retirement Thrift Investment Board... ADDRESS: Ethics Officer, Federal Retirement Thrift Investment Board, 77 K Street NE., Suite 1000...

  11. 75 FR 13090 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-03-18

    ... Management (IDM) System location: Defense Information Systems Agency (DISA), 401 East Moore Drive, Montgomery... Management (IDM) process and provides approval for new users to gain access to additional systems within DISA...

  12. 75 FR 56533 - Privacy Act System of Records

    Science.gov (United States)

    2010-09-16

    ... (CSMS)'' is to enable the FCC's Space Management Center (SMC) to use the CSMS information system to... SYSTEM NAME: Cadapult Space Management System (CSMS). SECURITY CLASSIFICATION: The FCC's Security.... PURPOSE(S): Space Management Center (SMC) uses the CSMS information system to allocate the offices...

  13. 77 FR 57077 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-17

    ... Intelligence Agency. Categories of Records in the System: Name, Social Security Number (SSN), work and home...; System of Records AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice to Add a System of Records. SUMMARY: The Defense Intelligence Agency is proposing the add a system to its existing inventory of...

  14. 75 FR 26201 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-11

    ... National Defense Intelligence College. Categories of records in the system: Name, date of birth and Social...; System of Records AGENCY: Defense Intelligence Agency, DoD. ACTION: Notice to amend a system of records. SUMMARY: The Defense Intelligence Agency is proposing to amend a system in its existing inventory of...

  15. 75 FR 20675 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-20

    ...--Office of Domestic Finance, Actuarial Valuation System DO .015--Political Appointee Files. DO .060..., Actuarial Valuation System--Treasury/ DO. System location: Departmental Offices, Office of Government... system is as follows: Active Records: Name; social security number; salary; category-grade; pay-plan...

  16. 76 FR 39390 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-07-06

    ...: Labor Management Relations Records System (May 19, 2009, 74 FR 23396) Changes: * * * * * System location: Delete entry and replace with ``Human Resources, Labor and Employee Relations Policy (J-13), Headquarters... Management Relations Records System. System location: Human Resources, Labor and Employee Relations Policy (J...

  17. 78 FR 14275 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ..., Washington, DC 20301-1155. Categories of individuals covered by the system: Military personnel, DoD civilian... information within the Enterprise Support Portal. To process network/system account requests, IT service... from the system manager. Record source categories: Defense Civilian Personnel Data System, Defense...

  18. 78 FR 41917 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-12

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting Service is amending a system of records... contained in this record system should address written inquiries to the Defense Finance and Accounting...

  19. 78 FR 41916 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-12

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to alter a System of Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records... INFORMATION: The Defense Finance and Accounting Service notices for systems of records subject to the Privacy...

  20. 77 FR 58106 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-19

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend two Systems of Records. SUMMARY: The Defense Finance and Accounting Service is amending two systems of records.... SUPPLEMENTARY INFORMATION: The Defense Finance and Accounting Service systems of records notices subject to the...

  1. 77 FR 60400 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-03

    ...; System of Records AGENCY: Defense Finance and Accounting Service. ACTION: Notice to delete two systems of records. SUMMARY: The Defense Finance and Accounting Service is deleting two systems of records notices in.... SUPPLEMENTARY INFORMATION: The Defense Finance and Accounting Service systems of records notices subject to the...

  2. 75 FR 21248 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-23

    ...; Systems of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to alter a system of records. SUMMARY: The Defense Finance and Accounting Service (DFAS) is proposing to alter a system...) 522-5225. SUPPLEMENTARY INFORMATION: The Defense Finance and Accounting Service notices for systems of...

  3. 78 FR 37799 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-06-24

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting Service is amending a system of records... 44199-2055.'' * * * * * System manager(s) and address: Delete entry and replace with ``Defense Finance...

  4. 78 FR 79412 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-12-30

    ... Defense Finance and Accounting Service proposes to alter a system of records, T7205, General Accounting and Finance System--Report Database for Financial Statements, in its inventory of record systems... INFORMATION: The Defense Finance and Accounting Service notices for systems of records subject to the Privacy...

  5. 78 FR 41918 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-12

    ...; System of Records AGENCY: Office of the Secretary, DoD. ACTION: Notice to alter a System of Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records, T7905, entitled... contained in this record system should address written inquiries to the Defense Finance and Accounting...

  6. 78 FR 52518 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-08-23

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to amend a System of Records. SUMMARY: The Defense Finance and Accounting Service is amending a system of records...-4591. SUPPLEMENTARY INFORMATION: The Defense Finance and Accounting Service systems of records notices...

  7. 78 FR 6078 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-29

    ...; System of Records AGENCY: Defense Finance and Accounting Service, DoD. ACTION: Notice to delete a system of records. SUMMARY: The Defense Finance and Accounting Service is deleting a system of records.... SUPPLEMENTARY INFORMATION: The Defense Finance and Accounting Service systems of records notices subject to the...

  8. 78 FR 61345 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-10-03

    ... Officer, Department of Defense. N01306-1 System Name: Career Management/Interactive Detailing System (CMS... with ``Career Management System-- Interactive Detailing (CMS-ID).'' System location: Delete entry and..., San Diego, California 92152-5001 during Contingency of Operations Planning (COOP).'' Categories of...

  9. 75 FR 54423 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-09-07

    ... Personnel System Treasury .002--Grievance Records Treasury .003--Treasury Child Care Tuition Assistance... sources such as private employers. Exemptions Claimed for the System: None. TREASURY .002 System name... Care Tuition Assistance Records--Treasury. System location: Department of the Treasury, 1500...

  10. 78 FR 5781 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-28

    ... users and organizations; and (6) an enterprise-class Customer Relationship Management platform to manage.... * Mail: Federal Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd Floor, Suite.... DSCA 03 System name: Regional Center Persons/Activity Management System (RCPAMS). System location...

  11. 75 FR 39502 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-07-09

    ... submitting comments. * Mail: Federal Docket Management System Office, 1160 Defense Pentagon, Washington, DC... patient administration. Routine uses of records maintained in the system, including categories of users...; System of Records AGENCY: Department of the Army, DoD. ACTION: Notice to amend a system of records...

  12. 77 FR 9902 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-02-21

    .... * Mail: Federal Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd Floor, Suite... patient transfer, and from the Military Health Information System.'' * * * * * [FR Doc. 2012-3814 Filed 2...; System of Records AGENCY: Department of the Air Force, DoD. ACTION: Notice to alter a system of records...

  13. SPRYSEC effector proteins in Globodera rostochiensis

    NARCIS (Netherlands)

    Rehman, S.

    2008-01-01

    Plant pathogens inject so-called effector molecules into the cells of a host plant to promote their growth and reproduction in these hosts. In plant parasitic nematodes, these effector molecules are produced in the salivary glands. The objective of this thesis was to identify and characterize

  14. 48 CFR 252.209-7006 - Limitations on Contractors Acting as Lead System Integrators.

    Science.gov (United States)

    2010-10-01

    ... System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions And Clauses 252.209-7006 Limitations on Contractors Acting... construction of any individual system or element of any system of systems. (c) Representations. (1) The offeror...

  15. 78 FR 22522 - Privacy Act of 1974: New System of Records

    Science.gov (United States)

    2013-04-16

    ... System--CSOSA-20. SYSTEM NAME: Biometric Verification System. SECURITY CLASSIFICATION: Unclassified... Privacy Act of 1974 (5 U.S.C. 552a), as amended, titled ``Biometric Verification System (CSOSA-20).'' This... Biometric Verification System allows individuals under supervision to electronically check-in for office...

  16. Lateral-Directional Parameter Estimation on the X-48B Aircraft Using an Abstracted, Multi-Objective Effector Model

    Science.gov (United States)

    Ratnayake, Nalin A.; Waggoner, Erin R.; Taylor, Brian R.

    2011-01-01

    The problem of parameter estimation on hybrid-wing-body aircraft is complicated by the fact that many design candidates for such aircraft involve a large number of aerodynamic control effectors that act in coplanar motion. This adds to the complexity already present in the parameter estimation problem for any aircraft with a closed-loop control system. Decorrelation of flight and simulation data must be performed in order to ascertain individual surface derivatives with any sort of mathematical confidence. Non-standard control surface configurations, such as clamshell surfaces and drag-rudder modes, further complicate the modeling task. In this paper, time-decorrelation techniques are applied to a model structure selected through stepwise regression for simulated and flight-generated lateral-directional parameter estimation data. A virtual effector model that uses mathematical abstractions to describe the multi-axis effects of clamshell surfaces is developed and applied. Comparisons are made between time history reconstructions and observed data in order to assess the accuracy of the regression model. The Cram r-Rao lower bounds of the estimated parameters are used to assess the uncertainty of the regression model relative to alternative models. Stepwise regression was found to be a useful technique for lateral-directional model design for hybrid-wing-body aircraft, as suggested by available flight data. Based on the results of this study, linear regression parameter estimation methods using abstracted effectors are expected to perform well for hybrid-wing-body aircraft properly equipped for the task.

  17. Vibrio cholerae T3SS effector VopE modulates mitochondrial dynamics and innate immune signaling by targeting Miro GTPases.

    Science.gov (United States)

    Suzuki, Masato; Danilchanka, Olga; Mekalanos, John J

    2014-11-12

    The cellular surveillance-activated detoxification and defenses (cSADD) theory postulates the presence of host surveillance mechanisms that monitor the integrity of common cellular processes and components targeted by pathogen effectors. Being organelles essential for multiple cellular processes, including innate immune responses, mitochondria represent an attractive target for pathogens. We describe a Vibrio cholerae Type 3 secretion system effector VopE that localizes to mitochondria during infection and acts as a specific GTPase-activating protein to interfere with the function of mitochondrial Rho GTPases Miro1 and Miro2. Miro GTPases modulate mitochondrial dynamics and interfering with this functionality effectively blocks innate immune responses that presumably require mitochondria as signaling platforms. Our data indicate that interference with mitochondrial dynamics may be an unappreciated strategy that pathogens use to block host innate immune responses that would otherwise control these bacterial infections. VopE might represent a bacterial effector that targets the cSADD surveillance response. Copyright © 2014 Elsevier Inc. All rights reserved.

  18. 75 FR 68849 - Privacy Act of 1974: System of Records

    Science.gov (United States)

    2010-11-09

    ... Institute, Aerospace Medical Certification Division, AAM-300; Regulatory Support Division, AFS-600; and... Program Tracking and Reporting System (PTRS), National Vital Information System (VIS), and the Drug...: (a) Provide basic airmen certification and qualification information to the public upon request...

  19. 77 FR 65564 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-29

    ... Medical, Health and Billing Records system is the authoritative source of patients' IHS medical records... authoritative records, nor are they considered part of the IHS Medical, Health and Billing Records system of...

  20. 76 FR 111 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-01-03

    ... the system: Storage: Electronic storage media. Retrievability: By individual's name and Social....'' * * * * * Categories of records in the system: Delete entry and replace with ``Individual's name, Social Security..., marketing evaluation, assessment of manpower trends and characteristics; and related statistical studies and...

  1. 75 FR 77044 - Financial Management Service; Privacy Act of 1974, as Amended; System of Records

    Science.gov (United States)

    2010-12-10

    ... DEPARTMENT OF THE TREASURY Financial Management Service; Privacy Act of 1974, as Amended; System of Records AGENCY: Financial Management Service, Treasury. ACTION: Withdrawal of a Privacy Act Notice... behalf of the Financial Management Service. DATES: December 10, 2010. FOR FURTHER INFORMATION CONTACT...

  2. School Accountability Systems and the Every Student Succeeds Act. Re:VISION

    Science.gov (United States)

    Martin, Mike

    2016-01-01

    The "Every Student Succeeds Act" (ESSA) replaced the "No Child Left Behind Act of 2001" (NCLB) in December 2015, substantially changing the federal role in education and how schools across the country will be held accountable. For state policymakers, designing new ESSA-compliant accountability systems is a significant…

  3. 75 FR 10554 - Privacy Act of 1974; System of Records Notice

    Science.gov (United States)

    2010-03-08

    ... Primary Data Center AT&T Data Center, Ashburn, VA 20147. NIC Secondary Data Center AT&T Data Center, Allen... (PSP), as required by 49 U.S.C. 31150. The system of records will make crash and inspection data about... to submit a Freedom of Information Act (FOIA) request or Privacy Act request to FMCSA for the data...

  4. 75 FR 78802 - Privacy Act of 1974, as Amended; System of Records

    Science.gov (United States)

    2010-12-16

    ... Financial Management Service Privacy Act of 1974, as Amended; System of Records AGENCY: Financial Management... Privacy Act of 1974, as amended, the Financial Management Service gives notice of a proposed new Privacy... should send your comments to Peter Genova, Deputy Chief Information Officer, Financial Management Service...

  5. 75 FR 73095 - Privacy Act of 1974; Report of New System of Records

    Science.gov (United States)

    2010-11-29

    ... Social Security Act (the Act) by establishing incentive payments to eligible professionals (EPs... this system will also be disclosed to: (1) Support regulatory, incentive payments and policy functions..., Division of Information Security and Privacy Management, Enterprise Architecture and Strategy Group, Office...

  6. 77 FR 60412 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-03

    ...: The Department of the Army is deleting a system of records notice in its existing inventory of record... Declassification Agency, 7701 Telegraph Road, Casey Building, Suite 144, Alexandria, VA 22315-3827 or by phone at... proposes to delete a systems of records notice from its inventory of record systems subject to the Privacy...

  7. 78 FR 14273 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ...: Training and Employee Development Record System (August 11, 2010, 75 FR 48655) Reason: The Defense Logistics Agency (DLA) systems of records notice, S335.01, Training and Employee Development Record System..., identified by docket number and title, by any of the following methods: * Federal Rulemaking Portal: http...

  8. 78 FR 14280 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records notice... media.'' * * * * * System manager(s) and address: Delete entry and replace with ``Defense Finance and... contained in this record system should address written inquiries to the Defense Finance and Accounting...

  9. 78 FR 41919 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-12

    .... SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records, T7340c... INFORMATION: The Defense Finance and Accounting Service notices for systems of records subject to the Privacy....'' * * * * * Purpose(s): Delete entry and replace with ``The system will enable the Defense Finance Accounting Service...

  10. 78 FR 14286 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Records. SUMMARY: The Defense Finance and Accounting Service proposes to amend a system of records in its... record system should address written inquiries to the Defense Finance and Accounting Service, Freedom of... record system should address written inquiries to Defense Finance and Accounting Service, Freedom of...

  11. 78 FR 5784 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-28

    ... records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records in its... contained in this record system should address written inquiries to the Defense Finance and Accounting... contained in this record system should address written inquiries to Defense Finance and Accounting Service...

  12. 78 FR 14285 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records in its..., 72 FR 8698). Changes: * * * * * System location: Delete entry and replace with ``Defense Finance... contained in this record system should address written inquiries to the Defense Finance and Accounting...

  13. 78 FR 31905 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-28

    ... Records. SUMMARY: The Defense Finance and Accounting Service proposes to alter a system of records in its... name: General Accounting and Finance System--Defense Transaction Interface Module (June 4, 2007, 72 FR... individuals covered by the system: Delete entry and replace with ``Defense Finance and Accounting Service...

  14. 76 FR 55057 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-09-06

    ... Records. System location: U.S. Army Human Resources Command, Education Incentives Branch, 1600 Spearhead... Reserve; Army Regulation 621-5, Army Continuing Education System; Army Regulations 621-202, Education...; System of Records AGENCY: Department of the Army, Department of Defense (DoD). ACTION: Notice to add a...

  15. 75 FR 21250 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-23

    .... CATEGORIES OF INDIVIDUALS COVERED BY THE SYSTEM: NSA Police Officers; civilian DoD employees; military... Federal Register Liaison Officer, Department of Defense. GNSA 20 SYSTEM NAME: NSA Police Operational Files..., Ft. George G. Meade, MD 20755-6000.'' * * * * * GNSA 2 SYSTEM NAME: NSA Police Operational Files...

  16. 76 FR 23810 - Privacy Act System of Records

    Science.gov (United States)

    2011-04-28

    ... Legislative Management Tracking System (LMTS) is retrieved by the correspondence log-in file number.../OLA-1, ``Legislative Management Tracking System (LMTS).'' The FCC's Office of Legislative Affairs (OLA...) in the Commission's Legislative Management Tracking System (LMTS). OLA uses LMTS to store, track, and...

  17. 75 FR 60437 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-09-30

    ... purpose of the Customer Relationship Management system is to support Air Force members and their... the Customer Relationship Management system. Retention and disposal: Accountability records... Federal Register Liaison Officer, Department of Defense. F065 AF FMP B System name: Customer Relationship...

  18. 76 FR 57989 - Privacy Act System of Records

    Science.gov (United States)

    2011-09-19

    ... COOP matters, etc.; and An automated telephone and e-mail system to contact its Emergency Contacts and... employees and contractors regarding COOP matters, etc.; and 3. An automated telephone and e-mail system to... 3. To allow the FCC to use an automated telephone and e-mail system to contact its Emergency...

  19. 77 FR 72336 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-05

    ....regulations.gov . Follow the instructions for submitting comments. Mail: Federal Docket Management System... CFSC System name: Army Club Membership Files (June 21, 2001, 66 FR 33239). Reason: The records were..., Army Club Membership Files system of records notice can be deleted. Records have met the required...

  20. 75 FR 34709 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-18

    ....regulations.gov . Follow the instructions for submitting comments. Mail: Federal Docket Management System... centers, hospitals and clinics; by custodian of the record system; and by person(s) responsible for servicing the record system in performance of their official duties and by authorized personnel who are...

  1. 77 FR 65938 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-31

    ...) is amending the system of records currently entitled ``Patient Medical Record--VA'' (24VA19) as set..., comments may be viewed online through the Federal Docket Management System (FDMS) at www.regulations.gov... entitled Caregivers. Categories of Records in the System is being amended to add Bed Management Solution...

  2. 78 FR 68039 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-13

    ... Division, Operations Branch Chief (ABREO), Analysis Directorate, National Geospatial- Intelligence Agency...; System of Records AGENCY: National Geospatial-Intelligence Agency, DoD. ACTION: Notice to add a new system of records. SUMMARY: The National Geospatial-Intelligence Agency is establishing a new system of...

  3. Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris

    Directory of Open Access Journals (Sweden)

    Guo-Feng Jiang

    2013-09-01

    Full Text Available It is well known that the type III secretion system (T3SS and type III (T3 effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2 which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2 is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME. Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.

  4. 78 FR 55703 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2013-09-11

    ... of individuals covered by the system: Records are maintained on individuals, including sole proprietorships, members of partnerships, and officers and owners of corporate licensees, managers and owners of...

  5. 75 FR 72873 - Privacy Act Of 1974; System of Records

    Science.gov (United States)

    2010-11-26

    ..., Texas 78772. Former Prisoner of War Statistical Austin Information Technology Tracking System. Center... Park Dr Suite 800 Morrisville, North Carolina 27560. Parkinson's Disease Research, Education Veterans...

  6. 77 FR 11063 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2012-02-24

    ... signature and encryption certificates for documents and email and to add biometric authentication... Security Office and Privacy Officer. USAID-31 System name: HSPD-12 PIV Lifecycle Management. Security...

  7. 75 FR 53000 - Privacy Act of 1974; Report of an Altered System of Records

    Science.gov (United States)

    2010-08-30

    ... 1974; Report of an Altered System of Records ACTION: Notice to amend a system of records. SUMMARY: The Peace Corps is revising two of its existing systems of records notices subject to the Privacy Act of... a report on this system to OMB and the Congress. Peace Corps is publishing changes which affect the...

  8. 76 FR 77575 - Privacy Act of 1974; Report of an Altered System of Records

    Science.gov (United States)

    2011-12-13

    ... 1974; Report of an Altered System of Records AGENCY: Notice to amend a system of record. SUMMARY: The Peace Corps is revising an existing systems of record notice subject to the Privacy Act of 1974, (5 U.S... the revision. In accordance with 5 U.S.C. 552a, Peace Corps has provided a report on this system to...

  9. 77 FR 65048 - Privacy Act; System of Records: Personal Property Claims, State-27

    Science.gov (United States)

    2012-10-24

    ... direct supervision of the system manager. The system manager has the capability of printing audit trails... system of records, Personal Property Claims, State-27, pursuant to the provisions of the Privacy Act of... State proposes that the current system will retain the name ``Personal Property Claims'' (42 FR 49715...

  10. Diversification of C. elegans Motor Neuron Identity via Selective Effector Gene Repression.

    Science.gov (United States)

    Kerk, Sze Yen; Kratsios, Paschalis; Hart, Michael; Mourao, Romulo; Hobert, Oliver

    2017-01-04

    A common organizational feature of nervous systems is the existence of groups of neurons that share common traits but can be divided into individual subtypes based on anatomical or molecular features. We elucidate the mechanistic basis of neuronal diversification processes in the context of C.elegans ventral cord motor neurons that share common traits that are directly activated by the terminal selector UNC-3. Diversification of motor neurons into different classes, each characterized by unique patterns of effector gene expression, is controlled by distinct combinations of phylogenetically conserved, class-specific transcriptional repressors. These repressors are continuously required in postmitotic neurons to prevent UNC-3, which is active in all neuron classes, from activating class-specific effector genes in specific motor neuron subsets via discrete cis-regulatory elements. The strategy of antagonizing the activity of broadly acting terminal selectors of neuron identity in a subtype-specific fashion may constitute a general principle of neuron subtype diversification. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Effects of land registration on validity of juridical acts (Emphasis on Functions of land registration System

    Directory of Open Access Journals (Sweden)

    Nasrin tabatabai hesari

    2015-12-01

    Full Text Available Guaranty of security of juridical act about lands is a more important difficulties in every country. Disregarding the independence of land registration system from the system of civil law and disregarding bases and functions of land registration system has resulted in making mistakes by judicial doctrine and precedent in order to propose proper suggestions for solving the problems related to land registration system and to sanction for juridical acts about lands. Whiles presentation every solution in this scope must be proper regarding to protective bases of owner and third parties and two functional characters of the land registration system including “informing” and “protective” features. land registration systems can be divided in constitutive registration system and confirmative one. This classification means land registration in any case, has a very effective role in credit of judicial act about of land although the degree of this effect is different depending on the system.

  12. Co-ordinate regulation of distinct host cell signalling pathways by multifunctional enteropathogenic Escherichia coli effector molecules.

    Science.gov (United States)

    Kenny, Brendan; Ellis, Sarah; Leard, Alan D; Warawa, Jonathan; Mellor, Harry; Jepson, Mark A

    2002-05-01

    Enteropathogenic Escherichia coli (EPEC) is a major cause of paediatric diarrhoea and a model for the family of attaching and effacing (A/E) pathogens. A/E pathogens encode a type III secretion system to transfer effector proteins into host cells. The EPEC Tir effector protein acts as a receptor for the bacterial surface protein intimin and is involved in the formation of Cdc42-independent, actin-rich pedestal structures beneath the adhered bacteria. In this paper, we demonstrate that EPEC binding to HeLa cells also induces Tir-independent, cytoskeletal rearrangement evidenced by the early, transient formation of filopodia-like structures at sites of infection. Filopodia formation is dependent on expression of the EPEC Map effector molecule - a protein that targets mitochondria and induces their dysfunction. We show that Map-induced filopodia formation is independent of mitochondrial targeting and is abolished by cellular expression of the Cdc42 inhibitory WASP-CRIB domain, demonstrating that Map has at least two distinct functions in host cells. The transient nature of the filopodia is related to an ability of EPEC to downregulate Map-induced cell signalling that, like pedestal formation, was dependent on both Tir and intimin proteins. The ability of Tir to downregulate filopodia was impaired by disrupting a putative GTPase-activating protein (GAP) motif, suggesting that Tir may possess such a function, with its interaction with intimin triggering this activity. Furthermore, we also found that Map-induced cell signalling inhibits pedestal formation, revealing that the cellular effects of Tir and Map must be co-ordinately regulated during infection. Possible implications of the multifunctional nature of EPEC effector molecules in pathogenesis are discussed.

  13. 75 FR 6000 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-02-05

    ... (301) 688-6527. SUPPLEMENTARY INFORMATION: The National Security Agency's systems of notices subject to... Director, Enterprise Information Technology Services, National Security Agency/Central Security Service, Ft...; Systems of Records AGENCY: National Security Agency/Central Security Service, DoD. ACTION: Notice to alter...

  14. 76 FR 54743 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-09-02

    ... personnel and their dependents, policies and procedures relative to patents, inventions, taxation, and land... are properly screened, cleared and trained. The system maintains data encryption, role based access...; 32 CFR part 505; or may be obtained from the system manager. Records Source Categories: Information...

  15. 75 FR 28242 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-20

    ...-Free Workplace Program Records (August 27, 1999; 64 FR 46889). CHANGES: SYSTEM IDENTIFIER: Delete entry... MAINTENANCE OF THE SYSTEM: Delete entry and replace with ``E.O. 12564, Drug-Free Federal Workplace; Public Law... inquiries to the Privacy Office, Headquarters, Defense Logistics Agency, ATTN: DGA, 8725 John J. Kingman...

  16. 76 FR 22682 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-22

    ... storage devices are protected by computer system software, which is Common Access Card (CAC) enabled... Government Reform, the Senate Committee on Homeland Security and Governmental Affairs, and the Office of... folders and computer storage media.'' * * * * * System Manager(s) and address: Delete entry and replace...

  17. 75 FR 41539 - Privacy Act Systems of Records Notice

    Science.gov (United States)

    2010-07-16

    ... the system: Share and account records; personal data regarding income and debts; payment or employment history; accounts payable records. Authority for maintenance of the system: 12 U.S.C. 1787. Purpose: The...-related master data, properly account for employee- related reimbursements and provide documentary support...

  18. 75 FR 71090 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-22

    ... FURTHER INFORMATION CONTACT address above. The proposed systems reports, as required by 5 United States... Circular No. A-130, ``Federal Agency Responsibilities for Maintaining Records About Individuals,'' dated... system: Military and civilian personnel assigned to the Air Force and Combatant Commands under the...

  19. 78 FR 19243 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-29

    ... departmental information for the system location and system manager. The routine uses of records maintained in... States Patent and Trademark Office, 600 Dulany Street, Alexandria, VA 22314; b. Franconia Warehouse... Office, 600 Dulany Street, Alexandria, VA 22314; c. Franconia Warehouse--Files Repository, 6808 Loisdale...

  20. 77 FR 58816 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-24

    .... ADDRESSES: You may submit comments, identified by docket number and title, by any of the following methods.... * Mail: Federal Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd Floor, Suite... obsolete. The records are now covered by the Defense Finance and Accounting Service system of records...

  1. 76 FR 1409 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-01-10

    ... Accounting Service records notice T7332, Defense Debt Management System (February 19, 2009, 74 FR 7665). [FR... number and/ Regulatory Information Number (RIN) and title, by any of the following methods: Federal...: Federal Docket Management System Office, Room 3C843, 1160 Defense Pentagon, Washington, DC 20301-1160...

  2. 75 FR 16760 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-02

    ... Disposing of Records in the System: Storage: Electronic storage media. Retrievability: Name and/or Social... Records in the System: Personnel data includes full name, Social Security Number (SSN), rank/grade, date... performance of their official duties. MCMPS is currently encrypted to protect the transmission of personal...

  3. 75 FR 37842 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-06-30

    ...: Individuals who are consultants and vendors to PBGC. Categories of records in the systems: Payment vouchers..., Human Resources Department; Director, Benefits Administration and Payment Department. Correspondence... system of records is maintained for use in determining amounts to be paid and in effecting payments by...

  4. 77 FR 51949 - Privacy Act, Exempt Record System

    Science.gov (United States)

    2012-08-28

    ... Research Integrity (ORI) immediately to ensure that FDA's Office of Criminal Investigations, HHS Office of... new system of records, 09-10-0020, ``FDA Records Related to Research Misconduct Proceedings, HHS/FDA... ``FDA Records Related to Research Misconduct Proceedings.'' The purpose of this system of records is to...

  5. 77 FR 70136 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2012-11-23

    .... It is USAID's core financial management system and accounting system of record. Phoenix enables USAID..., which are required to perform necessary accounting operations. Phoenix falls under strict regulatory...: name, social security number, details of payroll transactions and work phone numbers. Phoenix imports...

  6. 76 FR 52320 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-22

    ... & Privacy, and DoD Information Assurance Regulations. Auditing: Audit trail records from all available.../JS Privacy Office, Freedom of Information Directorate, Washington Headquarters Services, 1155 Defense... Defense. DHA 23 System name: Pharmacy Data Transaction Service (PDTS). System location: Primary: Emdeon...

  7. 76 FR 36903 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-06-23

    ... ``Individual's name, nick names, Social Security Number (SSN), gender, date of birth, personal cell phone.... Categories of records in the system: Individual's name, nick names, Social Security Number (SSN), gender... records in the system: Storage: Electronic storage media. Retrievability: Individual's name and/or Social...

  8. 77 FR 29619 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-18

    ... system: Name, Social Security Number (SSN), gender, race/ethnicity, birth date, place of birth, home..., spouse information, marital status, dependent child information (citizenship, gender, date of birth..., accessing, retaining, and disposing of records in the system: Storage: Electronic storage media...

  9. 75 FR 13091 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-03-18

    ... birth, gender, Social Security Number (SSN), rank/grade, duty status, skill specialty, and deployability... Reporting guidelines and procedures. Categories of records in the system: Name, date of birth, gender..., and disposing of records in the system: Storage: Electronic storage media. Retrievability: Individual...

  10. 76 FR 43278 - Privacy Act; System of Records

    Science.gov (United States)

    2011-07-20

    ... confirmed compromise there is a risk of harm to economic or property interests, identity theft or fraud, or... there is a risk of harm to economic or property interests, identity theft or fraud, or harm to the..., identity theft or fraud, or harm to the security or integrity of this system or other systems or programs...

  11. 77 FR 2721 - Privacy Act System of Records

    Science.gov (United States)

    2012-01-19

    ...-3 SYSTEM NAME: National Deaf-Blind Equipment Distribution Program (NDBEDP). SECURITY CLASSIFICATION: The FCC's Security Operations Center (SOC) has not assigned a security classification to this system...) The Commission suspects or has confirmed that the security or confidentiality of information in the...

  12. 76 FR 41497 - Privacy Act System of Records

    Science.gov (United States)

    2011-07-14

    ... Broadband Test. Security Classification: The FCC's Security Operations Center (SOC) has not assigned a security classification to this system of records. System Location: Office of Strategic Planning (OSP... persons when (1) The Commission suspects or has confirmed that the security or confidentiality of...

  13. 76 FR 35197 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-06-16

    ... Administrators (SAs) only. Server access is controlled by IP address. System is accessible world-wide. Servers... submissions available for public viewing on the Internet at http://www.regulations.gov as they are received... Police patrols, installation fences, key card access, building-server room alarms and cameras. System...

  14. 76 FR 79661 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-22

    ...: * * * * * System location: Epsilon Marketing Technology, Inc., 601 Edgewater Drive, Wakefield, MA 01880-6235... Advertising, Market Research & Studies (JAMRS) Survey Database. System location: Epsilon Marketing Technology..., Marketing Research & Studies (JAMRS), Attn: Survey Project Officer, Suite 06J25, 4800 Mark Center Drive...

  15. 76 FR 27103 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-10

    ... recipient in the event of a system modification or change to FederalReporting.gov , including the data..., authority or licensing organization may then make a request supported by the written consent of the... resistance to tampering and circumvention. This system limits data access to Board and contract staff on a...

  16. 77 FR 26254 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-03

    ... General. Categories of records in the system: Individual's name, Social Security Number (SSN), employee... requests for awards or promotions. Records are used to effect corrective personnel or other administrative... disposing of records in the system: Storage: Paper records and electronic storage media. Retrievability: By...

  17. 76 FR 49755 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-11

    ... aircraft. Categories of records in the system: Name, last four of Social Security Number (SSN), squadron ID... of Appendix I to OMB Circular No. A-130, ``Federal Agency Responsibilities for Maintaining records... Military Flight Operations Quality Assurance System location: Primary databases (Enterprise Level Servers...

  18. 75 FR 49482 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-13

    ..., Military Police Incident Reports, traffic tickets, letters of notification of dishonored checks, and... Civilian and Military Police Service Investigative Agencies, Military Exchange and Commissary systems, or... individuals covered by the system: Any personnel (military or civilian) assigned to DISA Europe involved in...

  19. 76 FR 82286 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-30

    ...: The Department of the Army is deleting a system of records notice from its existing inventory of... action will be effective without further notice on January 30, 2012 unless comments are received which... the instructions for submitting comments. * Mail: Federal Docket Management System Office, 4800 Mark...

  20. 78 FR 31909 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-28

    .... SUMMARY: The Department of the Army is deleting two systems of records notices in its existing inventory... proposed action will be effective on June 28, 2013 unless comments are received which result in a contrary....regulations.gov . Follow the instructions for submitting comments. Mail: Federal Docket Management System...

  1. 75 FR 67703 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-03

    .... SUMMARY: The Department of the Army is deleting a systems of record notice from its existing inventory of... action will be effective without further notice on December 3, 2010 unless comments are received which... Management System Office, Room 3C843, 1160 Defense Pentagon, Washington, DC 20301-1160. Instructions: All...

  2. 77 FR 4025 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-01-26

    ...: The U.S. Central Command is amending a system of records notice in its existing inventory of record... be effective on February 27, 2012 unless comments are received which result in a contrary... submitting comments. * Mail: Federal Docket Management System Office, 4800 Mark Center Drive, East Tower, 2nd...

  3. 78 FR 73515 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-12-06

    ... or retirement due to physical disability of Navy and Marine Corps personnel, by establishing the.... N01850-2 System Name: Physical Disability Evaluation System Proceedings (April 14, 1999, 64 FR 18410... disability. All Navy and Marine Corps personnel who have been considered by a Physical Evaluation Board for...

  4. 76 FR 37329 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-06-27

    ... has found contempt or an ethics violation, or the attorney has been disciplined elsewhere. Categories... prospective government employers. Routine uses of records maintained in the system, including categories of... employers. The DoD `Blanket Routine Uses' set forth at the beginning of the Army's compilation of systems of...

  5. 75 FR 69642 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-15

    ... name and password. Security systems and/or security guards protect buildings where records are accessed... and password. Security systems and/or security guards protect buildings where records are accessed or... information including Social Security Number (SSN), employee number, emergency contact information, employee e...

  6. 75 FR 67697 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-11-03

    ... Assurance Scholarship Program (IASP). CATEGORIES OF RECORDS IN THE SYSTEM: Individual information to include... Scholarship Program (IASP) to qualified applicants and institutions. This system is also used by management... officials to facilitate the recruiting of DoD IASP award recipients into Federal service for the purpose of...

  7. 78 FR 5789 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-28

    ... number, general accounting office exception code, date of separation, branch of service, assigned unit... for official need-to-know. System software uses Primary Key Infrastructure (PKI)/Common Access Card (CAC) authentication to lock out unauthorized access. System software contains authorization...

  8. 76 FR 66752 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-27

    ... SYSTEMS: STORAGE: Information is stored manually in file jackets and electronically in office automation.... SAFEGUARDS: The information is stored in safes, locked filing cabinets, and office automation equipment in a... AND DISPOSAL: Records in the system are retained and disposed of in accordance with NARA Job NCI-60-77...

  9. 75 FR 33792 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-15

    ... the system: Storage: Paper records and electronic storage media. Retrievability: By last name, Social... be effective without further notice on July 15, 2010 unless comments are received which result in a... civilians, military and contractors. Categories of records in the system: Individual's name, Social Security...

  10. 77 FR 64095 - Privacy Act of 1974, System of Records

    Science.gov (United States)

    2012-10-18

    ... user-authenticated, password- protected systems. All records are accessed only by authorized personnel..., that desire to collect, maintain or store personnel data under the scope of this system of records will..., emergency contact phone number, emergency contact alternate number, program areas of expertise, foreign...

  11. 76 FR 18191 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-01

    ... Biometric Identification Data System (May 6, 2009, 74 FR 20930) Changes: System ID: DMDC 10 Delete entry and... entry and replace with ``Personal data includes name, grade, Social Security Number (SSN), DoD ID number... (PIP) Program; DoD Instruction 5200.08, Security of DoD Installations and Resources; DoD 5200.08-R...

  12. 77 FR 25993 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-02

    ...). Categories of records in the system: Name, Social Security Number (SSN), date of birth, home address, place... foreign language capability; projected suspense information for personnel actions; job applications and..., accessing, retaining, and disposing of records in the system: Storage: Electronic storage media...

  13. 76 FR 24001 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-04-29

    ... enrolled in schools operated or funded by DoDEA, world-wide.'' Categories of records in the system: Delete... forms, pertinent family medical history, psychological or mental health and physical assessments and... funded by DoDEA, world-wide. Categories of records in the system: The records described in this notice...

  14. 75 FR 14142 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-03-24

    ... while traveling on official business; and to create a tracking system whereby DoD can monitor the... lodging for those individuals authorized to travel at government expense on official business. To the... traveling on official business; and to create a tracking system whereby DoD can monitor the authorization...

  15. 75 FR 57163 - Privacy Act Systems of Records

    Science.gov (United States)

    2010-09-20

    ... reflect concern about ODNI's action to exempt the new systems of records from the accounting, access... include an accounting of disclosures under subsection (c)(3). The systems notices, read in conjunction... exempt themselves from access challenges; ODNI agrees that [[Page 57165

  16. 78 FR 5792 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-28

    ... system: Delete entry and replace with ``Name, Social Security Number (SSN) and/or DoD ID Number, home... completing training; and E.O. 9397 (SSN), as amended.'' * * * * * Storage: Delete entry and replace with... manager listed above. The signed letter should contain full name and SSN and/or DoD ID Number. The system...

  17. 76 FR 58786 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-09-22

    ...), organization, work phone number, and records relating to the selection, notification, and testing of covered... with ``The system is used to maintain NSA/ CSS Drug Program Coordinator records on the selection... records, etc.) of employees and applicants for employment. Records contained in this system are also used...

  18. Oxysterols and Their Cellular Effectors

    Directory of Open Access Journals (Sweden)

    Eija Nissilä

    2012-02-01

    Full Text Available Oxysterols are oxidized 27-carbon cholesterol derivatives or by-products of cholesterol biosynthesis, with a spectrum of biologic activities. Several oxysterols have cytotoxic and pro-apoptotic activities, the ability to interfere with the lateral domain organization, and packing of membrane lipids. These properties may account for their suggested roles in the pathology of diseases such as atherosclerosis, age-onset macular degeneration and Alzheimer’s disease. Oxysterols also have the capacity to induce inflammatory responses and play roles in cell differentiation processes. The functions of oxysterols as intermediates in the synthesis of bile acids and steroid hormones, and as readily transportable forms of sterol, are well established. Furthermore, their actions as endogenous regulators of gene expression in lipid metabolism via liver X receptors and the Insig (insulin-induced gene proteins have been investigated in detail. The cytoplasmic oxysterol-binding protein (OSBP homologues form a group of oxysterol/cholesterol sensors that has recently attracted a lot of attention. However, their mode of action is, as yet, poorly understood. Retinoic acid receptor-related orphan receptors (ROR α and γ, and Epstein-Barr virus induced gene 2 (EBI2 have been identified as novel oxysterol receptors, revealing new physiologic oxysterol effector mechanisms in development, metabolism, and immunity, and evoking enhanced interest in these compounds in the field of biomedicine.

  19. 77 FR 9632 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-02-17

    ... computerized data is restricted by passwords, which are changed periodically or by Common Access Cards (CACs..., payroll office and payroll records, including automated payroll systems.'' * * * * * BILLING CODE 5001-06...

  20. 75 FR 20346 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-19

    ... containing contact information that individuals affiliated with an authorized entity provide to request... categories of individuals covered by this system to include individuals affiliated with secondary schools... program designed to improve access and promote enrollment in postsecondary education by facilitating...

  1. 76 FR 66696 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-27

    ... and Management Employee Relations Division, Human Resources Directorate, Washington Headquarters... address: Delete entry and replace with ``Assistant Director, Labor and Management Employee Relations... Records. System location: Labor and Management Employee Relations Division, Human Resources Directorate...

  2. 78 FR 22525 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-04-16

    ... Defense. S375.20 System name: Employee Relations under Negotiated Grievance Procedures (November 29, 2011... Logistics Agency Human Resources, Labor and Employee Relations Policy, 8725 John J. Kingman Road, Suite 3630...

  3. 78 FR 34354 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-06-07

    ..., accounting, promotions, career development, and procurement, including: name, Social Security Number (SSN... Activity Personnel Diaries, personnel accounting documents, and other personnel transaction documents... name, Social Security Number (SSN), rank, status, and signature of requester. The system manager may...

  4. 78 FR 65620 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-01

    ... support officer assignment, planning, programming, accounting, promotions, career development, and procurement, including: Name, Social Security Number (SSN), rank, status, education, training, security..., and number of dependants. The system also contains Activity Personnel Diaries, personnel accounting...

  5. 77 FR 28898 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-16

    ..., DC 20530, phone (202) 305-8840. SUPPLEMENTARY INFORMATION: Community policing is a philosophy that... identification features and other system protection methods also restrict access. Access is limited to those who...

  6. 76 FR 20339 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2011-04-12

    ... entry and replace with ``Individual's name, Social Security Number (SSN), gender, rank, Military..., Social Security Number (SSN), gender, rank, Military Occupational Specialty, duty station, roster ID... system: Storage: Records are maintained on paper and electronic storage media. Retrievability: Individual...

  7. 77 FR 34943 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-06-12

    ... document is the document published in the Federal Register. Free Internet access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System...

  8. 77 FR 41774 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-07-16

    ... document published in the Federal Register. Free Internet access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System at: www.gpo.gov...

  9. 77 FR 27756 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-11

    ... document is the document published in the Federal Register. Free Internet access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System...

  10. 77 FR 31606 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-29

    ... published in the Federal Register. Free Internet access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System at: www.gpo.gov/fdsys . At...

  11. 77 FR 37893 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-06-25

    ... access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System at: www.gpo.gov/fdsys . At this site you can view this document, as...

  12. 77 FR 76012 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-26

    ... is the document published in the Federal Register. Free Internet access to the official edition of the Federal Register and the Code of Federal Regulations is available via the Federal Digital System...

  13. 78 FR 22854 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-04-17

    ... administrative procedures. Computers are equipped with ``Smart Card'' technology that requires the insertion of an embedded identification card and entry of a PIN. All individuals granted access to this system of...

  14. 75 FR 70365 - Privacy Act Of 1974; System of Records

    Science.gov (United States)

    2010-11-17

    ... VA authoritative records, nor are they considered part of the VA system of records once they are..., the VA maintained program or application receiving the information will maintain the authoritative...

  15. 77 FR 57076 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-17

    ..., security clearance records, and employment records. Records include case files documenting the nature... system.'' Retention and disposal: Delete and replace with ``Inactive Litigation: Judicially contested... should contain the individual's full name, current address, and telephone number.'' Contesting record...

  16. 77 FR 25991 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-02

    ... provides synchronous and asynchronous access to training and education. Routine uses of records maintained... servicing the system in performance of their official duties. A risk assessment has been performed and will...

  17. 75 FR 136 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-01-04

    ... information management. It provides core functions required for resident student graduate education..., education planning, candidate packages, resource scheduling, degree auditing, financial reimbursements... (SSN), as amended. PURPOSE(S): This system integrates all aspects of student information management. It...

  18. 75 FR 21258 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-23

    ...; academic achievements, awards, honors, grades, and transcripts; performance counseling; health, physical... folders and electronic storage media. Retrievability: By surname or Social Security Number (SSN....'' Categories of records in the system: Delete entry and replace with ``Name, Social Security Number (SSN...

  19. 78 FR 27481 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-10

    ... Talent Management (HTM), Scholarships and Nursing Education Office, Veterans Health Administration..., sickle cell anemia or infection with the human immunodeficiency virus, that information cannot be... Archivist of the United States. SYSTEM MANAGER(S) AND ADDRESS: Director, Healthcare Talent Management...

  20. 75 FR 3714 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-01-22

    ..., identified by docket number and title, by any of the following methods: * Federal Rulemaking Portal: http.... Deletion DWHS P08. System name: Worker's Compensation-On-The-Job Injuries Report File (February 22, 1993...

  1. 75 FR 33789 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-15

    ... categories: Individual, social workers, rehabilitation counselors, and/or health care personnel. Exemptions... mental impairments and applicants for employment with Washington Headquarters Services/Human Resources... of records in the system: Storage: Paper files folders and electronic storage media. Retrievability...

  2. 76 FR 76956 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-09

    ... manager. Record source categories: Individual, social workers, rehabilitation counselors, and/or health... mental impairments and applicants for employment with Washington Headquarters Services/Human Resources... of records in the system: Storage: Paper file folders and electronic storage media. Retrievability...

  3. 75 FR 52513 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-26

    ... theater environmental monitoring data, personal protective equipment usage data, observation of work practices data, and employee health hazard educational data. Records include the name, Social Security... Records in the System: Storage: Paper records and electronic storage media. Retrievability: By individual...

  4. Mycobacterium tuberculosis effectors interfering host apoptosis signaling.

    Science.gov (United States)

    Liu, Minqiang; Li, Wu; Xiang, Xiaohong; Xie, Jianping

    2015-07-01

    Tuberculosis remains a serious human public health concern. The coevolution between its pathogen Mycobacterium tuberculosis and human host complicated the way to prevent and cure TB. Apoptosis plays subtle role in this interaction. The pathogen endeavors to manipulate the apoptosis via diverse effectors targeting key signaling nodes. In this paper, we summarized the effectors pathogen used to subvert the apoptosis, such as LpqH, ESAT-6/CFP-10, LAMs. The interplay between different forms of cell deaths, such as apoptosis, autophagy, necrosis, is also discussed with a focus on the modes of action of effectors, and implications for better TB control.

  5. The Shigella flexneri OspB effector: an early immunomodulator.

    Science.gov (United States)

    Ambrosi, Cecilia; Pompili, Monica; Scribano, Daniela; Limongi, Dolores; Petrucca, Andrea; Cannavacciuolo, Sonia; Schippa, Serena; Zagaglia, Carlo; Grossi, Milena; Nicoletti, Mauro

    2015-01-01

    Through the action of the type three secretion system (T3SS) Shigella flexneri delivers several effectors into host cells to promote cellular invasion, multiplication and to exploit host-cell signaling pathways to modulate the host innate immune response. Although much progress has been made in the understanding of many type III effectors, the molecular and cellular mechanism of the OspB effector is still poorly characterized. In this study we present new evidence that better elucidates the role of OspB as pro-inflammatory factor at very early stages of infection. Indeed, we demonstrate that, during the first hour of infection, OspB is required for full activation of ERK1/2 and p38 MAPKs and the cytosolic phospholipase A(2) (cPLA(2)). Activation of cPLA(2) ultimately leads to the production and secretion of PMN chemoattractant metabolite(s) uncoupled with release of IL-8. Moreover, we also present evidence that OspB is required for the development of the full and promptly inflammatory reaction characteristic of S. flexneri wild-type infection in vivo. Based on OspB and OspF similarity (both effectors share similar transcription regulation, temporal secretion into host cells and nuclear localization) we hypothesized that OspB and OspF effectors may form a pair aimed at modulating the host cell response throughout the infection process, with opposite effects. A model is presented to illustrate how OspB activity would promote S. flexneri invasion and bacterial dissemination at early critical phases of infection. Copyright © 2014 Elsevier GmbH. All rights reserved.

  6. Diverse Secreted Effectors Are Required for Salmonella Persistence in a Mouse Infection Model

    Energy Technology Data Exchange (ETDEWEB)

    Kidwai, Afshan S.; Mushamiri, Ivy T.; Niemann, George; Brown, Roslyn N.; Adkins, Joshua N.; Heffron, Fred

    2013-08-12

    Salmonella enterica serovar Typhimurium causes typhoid-like disease in mice and is a model of typhoid fever in humans. One of the hallmarks of typhoid is persistence, the ability of the bacteria to survive in the host weeks after infection. Virulence factors called effectors facilitate this process by direct transfer to the cytoplasm of infected cells thereby subverting cellular processes. Secretion of effectors to the cell cytoplasm takes place through multiple routes, including two separate type III secretion (T3SS) apparati as well as outer membrane vesicles. The two T3SS are encoded on separate pathogenicity islands, SPI-1 and -2, with SPI-1 more strongly associated with the intestinal phase of infection, and SPI-2 with the systemic phase. Both T3SS are required for persistence, but the effectors required have not been systematically evaluated. In this study, mutations in 48 described effectors were tested for persistence. We replaced each effector with a specific DNA barcode sequence by allelic exchange and co-infected with a wild-type reference to calculate the ratio of wild-type parent to mutant at different times after infection. The competitive index (CI) was determined by quantitative PCR in which primers that correspond to the barcode were used for amplification. Mutations in all but seven effectors reduced persistence demonstrating that most effectors were required. One exception was CigR, a recently discovered effector that is widely conserved throughout enteric bacteria. Deletion of cigR increased lethality, suggesting that it may be an anti-virulence factor. The fact that almost all Salmonella effectors are required for persistence argues against redundant functions. This is different from effector repertoires in other intracellular pathogens such as Legionella.

  7. Diverse secreted effectors are required for Salmonella persistence in a mouse infection model.

    Directory of Open Access Journals (Sweden)

    Afshan S Kidwai

    Full Text Available Salmonella enterica serovar Typhimurium causes typhoid-like disease in mice and is a model of typhoid fever in humans. One of the hallmarks of typhoid is persistence, the ability of the bacteria to survive in the host weeks after infection. Virulence factors called effectors facilitate this process by direct transfer to the cytoplasm of infected cells thereby subverting cellular processes. Secretion of effectors to the cell cytoplasm takes place through multiple routes, including two separate type III secretion (T3SS apparati as well as outer membrane vesicles. The two T3SS are encoded on separate pathogenicity islands, SPI-1 and -2, with SPI-1 more strongly associated with the intestinal phase of infection, and SPI-2 with the systemic phase. Both T3SS are required for persistence, but the effectors required have not been systematically evaluated. In this study, mutations in 48 described effectors were tested for persistence. We replaced each effector with a specific DNA barcode sequence by allelic exchange and co-infected with a wild-type reference to calculate the ratio of wild-type parent to mutant at different times after infection. The competitive index (CI was determined by quantitative PCR in which primers that correspond to the barcode were used for amplification. Mutations in all but seven effectors reduced persistence demonstrating that most effectors were required. One exception was CigR, a recently discovered effector that is widely conserved throughout enteric bacteria. Deletion of cigR increased lethality, suggesting that it may be an anti-virulence factor. The fact that almost all Salmonella effectors are required for persistence argues against redundant functions. This is different from effector repertoires in other intracellular pathogens such as Legionella.

  8. 78 FR 63455 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-10-24

    ... restricted by the use of lock, guards, and administrative procedures. Physical and electronic access is... performance of their duties. Once access is gained, the system is set with an automatic timeout period to... authorized personnel. Each machine is physically secured with a combination lock and cable. While the...

  9. 76 FR 114 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-01-03

    ... Government. Any DCAA employee who has requested an ethics opinion regarding the propriety of future actions..., Joint Ethics Regulation (JER); and E.O. 12731, Principles of Ethical Conduct for Government Officers and..., retaining, and disposing of records in the system: Storage: Paper records in file folders. Retrievability...

  10. 78 FR 23810 - Privacy Act System of Records

    Science.gov (United States)

    2013-04-22

    ...: Individuals who must create an account will furnish their Company's name, the authorized user's name, the..., INCLUDING CATEGORIES OF USERS AND THE PURPOSES OF SUCH USES, THESE RECORDS MAY BE USED, DISCLOSED OR... engaged by SBA to assist in the performance of a service related to this system of records and who need...

  11. 77 FR 65939 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-31

    ... prescription drug monitoring program, or similar program, for the purpose of submitting to or receiving from... prescription drug monitoring program (PMDP) for monitoring narcotics. The Report of Intent to Amend a System of... disclose relevant provider information to a state prescription drug monitoring program, or similar program...

  12. 77 FR 21974 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-04-12

    .... ADDRESSES: You may submit comments, identified by docket number and title, by any of the following methods... system: Delete entry and replace with ``Federal employees, contractors and active duty service members who access the Joint Intelligence Virtual University (JIVU II) in order to facilitate a training...

  13. 78 FR 69651 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-20

    ... 20340-0001. ] Categories of individuals covered by the system: DIA civilians, military assignees, and..., Intelligence Community Equal Employment; DoDD 1440.1, The DoD Civilian Equal Employment Opportunity Program; Do... denied any right, privilege, or benefit for which he would otherwise be entitled by Federal law or which...

  14. 78 FR 31526 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-24

    ... Agency Responsibilities for Maintaining Records About Individuals,'' dated February 8, 1996 (February 20... passes, payroll information, Social Security Number (SSN), time and attendance, performance, awards and... employment with NGA.'' System manager(s) and address: Delete entry and replace with ``Corporate Applications...

  15. 76 FR 73602 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-29

    ...: Employee Relations under Negotiated Grievance Procedures (August 7, 2009, 74 FR 39649). Changes..., Suite 1644, Fort Belvoir, VA 22060-6221.'' * * * * * S375.20 System name: Employee Relations under... connection with employee appeals from adverse actions and formal discrimination complaints...

  16. 76 FR 5351 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-01-31

    ... associated fees, personal resumes, previous schools attended, school contracts, theses, training achievements... records, course attendance and completion records, education level, test scores, transcript registry and...: schools; AR 621-5, Army Continuing Education System and E.O. 9397 (SSN), as amended.'' Purpose(s): Delete...

  17. 78 FR 44100 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-23

    ... System (LMS) for training and development programs; for the purpose of reporting, tracking, assessing and..., Operation of the DoD Financial Management Certification Program. Purpose(s): To manage and administer a... needs; for the purpose of reporting, tracking, assessing and monitoring training events, and DoD FM...

  18. 75 FR 2118 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-01-14

    ...: Maintained on computer and electronic storage media. RETRIEVABILITY: Retrieved by name and Social Security... cleared for need-to-know. Records are stored in locked rooms and on computer storage devices protected by....'' * * * * * CATEGORIES OF RECORDS IN THE SYSTEM: Delete entry and replace with ``Name, Social Security Number (SSN...

  19. 77 FR 74176 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-12-13

    ... devices are protected by computer system software.'' Retention and disposal: Delete entry and replace with... Oversight and Government Reform, the Senate Committee on Homeland Security and Governmental Affairs, and the...: Delete entry and replace with ``Full name, Social Security Number (SSN), rank, office address, work and...

  20. 76 FR 72325 - Privacy Act; Exempt Record System

    Science.gov (United States)

    2011-11-23

    ... by a reporting entity, whereas a data bank query is a request for information on a practitioner. Practitioners receive a copy of all reports submitted by a reporting entity along with instructions on... requires each State to adopt a system of reporting to the Secretary certain adverse licensure actions taken...

  1. 78 FR 14293 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... of Appendix I to OMB Circular No. A-130, ``Federal Agency Responsibilities for Maintaining Records... ``Air Force Active Duty, Reserve, Air National Guard personnel, and government civilians. Records are... Action Planning and Execution Segments (DCAPES) is the United States Air Forces system of records for...

  2. 78 FR 73508 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-12-06

    ... support the United States Air Force Museum System. DATES: This proposed action will be effective on... Circular No. A-130, ``Federal Agency Responsibilities for Maintaining Records About Individuals,'' dated..., citizenship, military and/or federal civilian service information, and private and professional affiliations...

  3. 76 FR 26714 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-09

    ... paragraph 4c of Appendix I to OMB Circular No. A-130, ``Federal Agency Responsibilities for Maintaining... government civilians employed by the Army.'' Categories of records in the system: Delete entry and replace... accordance with 28 U.S.C. 1746, in the following format: If executed outside the United States: `I declare...

  4. 76 FR 28964 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-19

    ... N. Brand Boulevard, Suite 700, Glendale, CA 91203- 3946. Secondary locations: Commandant of the...)-- Password controlled system, file, and element access based on predefined need-to-know basis. Computer.... Brand Boulevard, Suite 700, Glendale, CA 91203-3946. Secondary locations: Commandant of the Marine Corps...

  5. 75 FR 52944 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-30

    ... and the Division of Bank Regulation, Federal Housing Finance Agency, 1625 Eye Street, NW., Washington... Corporation, and the Federal Home Loan Banks (collectively, ``regulated entities''). This system is being...,'' in the subject line of the message. Federal eRulemaking Portal: http://www.regulations.gov . Follow...

  6. 75 FR 61865 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-06

    ... economic or property interests, identity theft or fraud, or harm to the programs (whether maintained by VA... Centers, the VA Records Management Center, St. Louis, Missouri, and at the Corporate Franchise Data Center... the Corporate Franchise Data Center in Austin, Texas. CATEGORIES OF RECORDS IN THE SYSTEM: The record...

  7. 77 FR 24982 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-04-26

    ... mental health after-care for current federal inmates. This routine use is needed to permit sharing of... for the purpose of securing medical or mental health after-care for current federal inmates. (u) To...: Federal Bureau of Prisons, Department of Justice. ACTION: Modified System of Records. SUMMARY: Pursuant to...

  8. 75 FR 24931 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-06

    ... the enforcement of criminal laws. Records maintained solely for statistical research or program... Enforcement Defense Data Exchange. SYSTEM LOCATION: Naval Criminal Investigative Service, 716 Sicard St... suspected of being involved in a crime incident or criminal investigation. In addition, individuals who...

  9. 77 FR 38342 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-06-27

    ... accountable property, carpool membership, and use of USPS parking facilities: Vice President, Facilities... product-specific service or support issues. SYSTEM MANAGER(S) AND ADDRESS: [CHANGE TO READ] Vice President... entered; type (credit card, ACH, check, etc.), amount, and date of postage purchases; ascending and...

  10. 77 FR 837 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-01-06

    ..., employee applicants, visitors to the bureau, news-media correspondents, contractor and service company..., alphabetically by name, social security number, alphabetically by company name, and numerically by passport... covered in the system to include ``employees or contractors of companies to which samples or test decks of...

  11. 78 FR 22526 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-04-16

    ... submitted claims for compensation under Public Law 104-201, Sections 657, as amended by Public Law 105- 261...://www.regulations.gov as they are received without change, including any personal identifiers or contact... Compensation Files (November 30, 1998, 63 FR 65760). Changes: System Identifier: Delete entry and replace with...

  12. 76 FR 3115 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-01-19

    ... data containing Social Security Number (SSN), name, gender, date of birth, home address, home phone... system: Storage: Delete entry and replace with ``Electronic storage media.'' Retrievability: Delete entry... Social Security Number (SSN), name, gender, date of birth, home address, home phone, work phone, work e...

  13. 75 FR 71081 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-22

    ..., address, phone numbers, date of birth, gender, current status (actively employed or retired), Social..., retaining and disposing of records in the system: Storage: Electronic storage media. Retrievability... media on which the data was stored. Some data is received in paper forms. This data is transferred to...

  14. 75 FR 67699 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-11-03

    ...; gender; religious code; race; company code; enlistment bonus; training phase; mandatory release date...: Paper records and electronic storage media. RETRIEVABILITY: By name or Social Security Number (SSN) of... destroy after 6 years by shredding or erasing from electronic storage media. SYSTEM MANAGER(S) AND ADDRESS...

  15. 77 FR 37004 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-06-20

    ... Geospatial-Intelligence Agency Maritime Safety Office Metrics Database. System location: Records are... are limited to government employees in the NGA Source Operations Directorate, Maritime Safety Office... Regulations. Purpose(s): The Maritime Safety Office collects, uses, maintains, and disseminates information to...

  16. 77 FR 56815 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-09-14

    ....regulations.gov as they are received without change, including any personal identifiers or contact information... military service as a career or a short-term employment option. Categories of records in the system... service, training performance, and demographic values. Scheduled data for follow-up data collections...

  17. 78 FR 14279 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... Logistics Agency, ATTN: DGA, 8725 John J. Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221, or by phone... Fire and Emergency Services Program Records (May 7, 2010; 75 FR 25213) S600.30 System name: Safety, Health, Injury, and Accident Records (February 5, 2010; 75 FR 5997) Reason: DLA is currently using and...

  18. 75 FR 20580 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-04-20

    .... Kingman Road, Suite 1644, Fort Belvoir, VA 22060-6221. The specific changes to the record system being... past five years and any motor vehicle accidents within the past five years, training and performance... with ``23 U.S.C. Chapter 4, Highway Safety; DoD Instruction 6055.4, DoD Traffic Safety Program; DoD...

  19. 78 FR 2695 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-14

    ..., Purpose, Retrievability, System Manager(s) and Address, Notification Procedure, and Record Source... information, finance number(s), duty location, and pay location. 2. Non-employee information: Name, gender.... * * * * * RETRIEVABILITY: [CHANGE TO READ] By employee and non-employee name, subject category, facility, finance number...

  20. 75 FR 25219 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-07

    ... SYSTEM: Badge and vehicle control records that at a minimum include: Name, Social Security Number (SSN... submissions available for public viewing on the Internet at http://www.regulations.gov as they are received... plate number, driver's license number, vehicle make, model, year, color, driver's identification...

  1. 75 FR 39500 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-07-09

    ... with ``Badge and vehicle control records that at a minimum include; name, Social Security Number (SSN... system: Badge and vehicle control records that at a minimum include; name, Social Security Number (SSN... Internet at http://www.regulations.gov as they are received without change, including any personal...

  2. 75 FR 36640 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-06-28

    ... Internet at http://www.regulations.gov as they are received without change, including any personal....'' Categories of records in the system: Delete entry and replace with ``Name, last four of Social Security...; 5 U.S.C. 7905, Programs to encourage commuting by means other than single-occupancy motor vehicles...

  3. 75 FR 64713 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-20

    ...'s name, Social Security Number (SSN), parking permit number, and vehicle tag number. Safeguards... viewing on the Internet at http://www.regulations.gov as they are received without change, including any... Reservation Vehicle Parking Program. System Location: Parking Management Branch, Pentagon Force Protection...

  4. 77 FR 13574 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-03-07

    ... Internet at http://www.regulations.gov as they are received without change, including any personal... commuting to and from work. Categories of records in the system: Name, last four of Social Security Number..., Programs to encourage commuting by means other than single-occupancy motor vehicles; DoD Instruction 1000...

  5. 75 FR 60431 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-09-30

    ... viewing on the Internet at http://www.regulations.gov as they are received without change, including any... events, vehicle collisions/rollovers and/or direct blows to the head, or witnessed loss of consciousness in their Area of Responsibility (AOR). Categories of Records in the System: Name, Social Security...

  6. 77 FR 271 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-01-04

    ... submissions available for public viewing on the Internet at http://www.regulations.gov as they are received....'' * * * * * Categories of records in the system: Delete entry and replace with ``Name; Social Security Number (SSN); home... date; badge number; vehicle ID and decal number; special intelligence access; expiration date, agency...

  7. 77 FR 5860 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-02-06

    ... working on a contract, service, grant, cooperative agreement, job, or other activity for the Board and who... entity, authority, or licensing organization may then make a request supported by the written consent of... of resistance to tampering and circumvention. This system limits data access to Board and contract...

  8. 77 FR 29616 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-18

    ... related to the DIB CS/IA program, cyber threat information and best practices, and mitigation strategies... security guards, identification badges, key cards, cipher locks, and combination locks. Retention and... Administration has approved the retention and disposition schedule). System manager(s) and address: Director, DIB...

  9. 77 FR 69442 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-11-19

    ... Department of the Army, Sexual Assault Data Management System, Army G-1, DAPE-HR-HF, Room 300 Army Pentagon...), Sexual Harassment/Assault Response and Prevention (SHARP), 2530 Crystal Drive, 6th Floor, Arlington, VA... Department of the Army, HRPD, Sexual Harassment/Assault Response and Prevention (SHARP), 2530 Crystal Drive...

  10. 77 FR 37002 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-06-20

    ... of the Army, Sexual Assault Data Management System, Army G-1, DAPE-HR-HF, Room 300 Army Pentagon... listed below: The Department of the Army, Human Resources Policy Directorate (HRPD), Sexual Harassment... written inquiries to the following as appropriate: The Department of the Army, HRPD, Sexual Harassment...

  11. 75 FR 52515 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-08-26

    ... equipped with ``Smart Card'' technology that requires the insertion of an embedded identification card and... system-generated, forced password-change protocols and also equipped with ``Smart Card'' technology that requires the insertion of an embedded identification card and entry of a PIN. In addition, computer screens...

  12. 75 FR 22187 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-27

    ... Vocational Rehabilitation and Employment Records--VA (58VA21/22/28).'' VA is amending the system of records... ; by mail or hand-delivery to the Director, Regulations Management (02REG), Department of Veterans..., Education, and Vocational Rehabilitation and Employment Records--VA'' (58VA21/22/28), published in the...

  13. Acting together in and beyond the mirror neuron system

    NARCIS (Netherlands)

    Kokal, Idil; Gazzola, Valeria; Keysers, Christian

    2009-01-01

    Moving a set dinner table often takes two people, and doing so without spilling the glasses requires the close coordination of the two agents' actions. It has been argued that the mirror neuron system may be the key neural locus of such coordination. Instead, here we show that such coordination

  14. 76 FR 28757 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-18

    ... online tool helps the staff members at all four levels (school, district, area, and headquarters) apply... to paragraph 4c of Appendix I to OMB Circular No. A-130, ``Federal Agency Responsibilities for.... Categories of records in the system: Records consist of first name, last name, Social Security Number (SSN...

  15. 76 FR 60008 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-09-28

    ... Liaison Officer, Department of Defense. Deletion: A0621-1 DAPE, Army Continuing Education Program Reason: The A0621-1 DAPE, Army Continuing Education Program is now covered under a new system of records notice, A0621-1a DAPE, Student Loan Repayment Program Records, (September 6, 2011, 76 FR 55057-55059) due...

  16. 75 FR 63824 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-18

    ... and replace with ``10 U.S.C. 8013, Secretary of the Air Force; 10 U.S.C. 47, Uniform Code of Military... Code of Military Justice and 8012, 8013; DoD Directive 7730.47, Defense Incident Based Reporting System... obtained from previous employers, financial institutions, educational institutions, police and...

  17. 77 FR 69445 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-11-19

    ... and replace with ``Military Police Reporting Program Records (MPRP).'' System location: Delete entry and replace with ``Decentralized to Army installations which created the Military Police Report... notices. The official copy of the military police report and other law enforcement related documents are...

  18. 76 FR 60812 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-09-30

    ... covered by the system: Delete entry and replace with ``Military personnel who are active duty; applicants... Officers' Training Corps (ROTC) cadets; former military personnel; and individuals residing on, have... (AISs). Attachments to ROIs or AISs including exhibits, subject or interviewee statements, police...

  19. 75 FR 63161 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-10-14

    ... and/or security or military police guards secure all locations. Only authorized personnel with a valid... non-duty hours, alarms systems and/or security or military police guards secure all locations. Only... States regarding certain military personnel office actions as provided for in DoD Instruction 1320.4...

  20. 75 FR 39499 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-07-09

    ... storage media. Retrievability: Name and/or Social Security Number (SSN) and Unit. Safeguards: Records are... system: Full name, Social Security Number (SSN), date of birth, grade, correspondence and records..., education, training, labor management relations, worker compensation, performance based actions, business...

  1. 75 FR 35853 - Privacy Act of 1974: Systems of Records

    Science.gov (United States)

    2010-06-23

    ...: Chief Information Officer, Office of Information Technology, Securities and Exchange Commission... INFORMATION CONTACT: Barbara A. Stance, Chief Privacy Officer, Office of Information Technology, 202-551-7209... suspected or confirmed that the security or confidentiality of information in the system of records has been...

  2. 77 FR 39346 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-07-02

    ... purposes of administering the Earned Income Tax Credit Program (Section 32, Internal Revenue Code of 1986... 27VA047 must be amended to accommodate this migration and consolidation. Specifically, the ``Systems... to the Department of the Treasury, Internal Revenue Service (IRS), where required by law or...

  3. 75 FR 43499 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-07-26

    ... maintained in a secure, password protected electronic system that utilizes security hardware, software, and... Reform, the Senate Committee on Homeland Security and Governmental Affairs, and the Office of Management..., rank/ grade, Military Occupational Specialty (MOS), Social Security Number (SSN), date of birth...

  4. 76 FR 51002 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-17

    ... with visual dysfunction related to traumatic brain injury, with an eye injury and a visual acuity in... of visual field in the injured eye. Categories of records in the system: Individual's full name... individual's service and employment information, including rank, service branch, job category, and disability...

  5. 78 FR 31907 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-05-28

    ... statement, legal guardianship, court martial correspondence, sex offender and DNA requirements and processing, classification, disciplinary and observation records, clothing and equipment, education and....O. 9397 (SSN), as amended.'' Purpose(s): Delete entry and replace with ``The system is used for...

  6. 75 FR 26851 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-12

    ... records or copies of records are maintained in a centralized electronic database at the Austin Automation... computer disk in a centralized database system. Paper records of program Participation Agreements and... information. Access to backup copies of data is restricted to authorized personnel in the same manner as the...

  7. 78 FR 14290 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    .... Street West, Randolph AFB, TX 78150-4750. Records are also located at Air Force units of assignment...D ID Number), rank, date of birth, duty phone, height, weight, physical fitness test scores... are properly screened and cleared for need-to-know. System software uses Primary Key Infrastructure...

  8. 77 FR 43816 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-07-26

    ... ID Number, AF Portal ID, student registrations and history of learning assets (courses, test... destroy.'' SYSTEM MANAGER(S) AND ADDRESS: Delete entry and replace with ``Cyberspace Support Squadron..., Cyberspace Support Squadron, Training Flight (CYSS/DOT), 203 West Losey Street, Scott AFB, IL 62225. For...

  9. 77 FR 65539 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-10-29

    ... replace with ``Washington Headquarters Services (WHS) records: Freedom of Information Division, Executive... 80011-9066.'' * * * * * System manager(s) and address: Delete entry and replace with ``WHS records... should address written inquiries to: WHS records: Chief, OSD/JS Privacy Office, Office of Freedom of...

  10. 76 FR 40343 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-07-08

    ..., diverted, or sanctioned the attorney, or has found contempt or an ethics violation, or the attorney has... to current and prospective government employers. Routine uses of records maintained in the system... prospective government employers. The DoD `Blanket Routine Uses' set forth at the beginning of the Army's...

  11. 76 FR 13997 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-15

    ... responsibility to professional licensing authorities and to current and prospective government employers... government employers. The DoD `Blanket Routine Uses' set forth at the beginning of the Army's compilation of... ethics violation, or the attorney has been disciplined elsewhere. Categories of records in the system...

  12. 76 FR 67155 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-31

    ... maintenance of the system: 10 U.S.C. 5013, Secretary of the Navy; DoD 8500.2, Information Assurance (IA) Implementation; and SECNAVINST 5239.3B, Department of the Navy Information Assurance Policy. Purpose(s): The... flag officers, internal and external staff coordination for the OPNAV staff; and other web services...

  13. 78 FR 43866 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-07-22

    ... OSD Federal Register Liaison Officer, Department of Defense. Deletion: K700.01, Employee-Management Relations and Services File (August 9, 1993, 58 FR 42302). Reason: Based on a recent review of the system of records notice K700.01, Employee-Management Relations and Services File, it has been determined that it is...

  14. 76 FR 12084 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-04

    ... provide flying career background information used for assignment actions. Accounting and Finance Office.... Records stored in computer storage devices are protected by computer system software. Computer terminals... information used for assignment actions. Accounting and Finance Office uses Military Pay Orders, prepared by...

  15. 76 FR 39392 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-07-06

    ....regulations.gov . Follow the instructions for submitting comments. Mail: Federal Docket Management System...: Mr. Leroy Jones, Department of the Army, Privacy Office, U.S. Army Records Management and... address, counselor's phone number and email, documentary evidence, affidavits, information from individual...

  16. 77 FR 27740 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-11

    ... storage media''. Retrievability: Delete ``and Social Security Number''. Safeguards: Delete last sentence... be effective on June 11, 2012 unless comments are received which result in a contrary determination... admission to, DLA-managed day care facilities.'' Categories of records in the system: Delete ``Social...

  17. 76 FR 76958 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-12-09

    ... electronic storage media. Retrievability: Information is retrieved by last name of recipient, Social Security... that would result in a contrary determination. ADDRESSES: You may submit comments, identified by docket... individual's name, grade, service, Social Security Number (SSN) and be signed.'' * * * * * DWHS P18 System...

  18. 75 FR 14141 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-03-24

    ... electronic storage media. RETRIEVABILITY: Individual's name, Social Security Number (SSN), or date of birth... result in a contrary determination. ADDRESSES: You may submit comments, identified by docket number and... facilities. CATEGORIES OF RECORDS IN THE SYSTEM: Subject individual's full name, Social Security Number (SSN...

  19. 78 FR 18565 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-27

    ... storage media. Retrievability: By individual's surname or Social Security Number. Safeguards: Records are... action will be effective on April 29, 2013 unless comments are received which result in a contrary... personnel. Categories of records in the system: Files contain individual's name, home address, Social...

  20. 75 FR 14579 - Privacy Act of 1974; Systems of Records

    Science.gov (United States)

    2010-03-26

    ... in the system: Storage: Paper and electronic storage media. Retrievability: Name, Social Security... effective without further notice on April 26, 2010 unless comments are received that would result in a... containing: Full name of the individual, Social Security Number (SSN), Deployment Identification Number, home...

  1. 76 FR 63287 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-12

    ... including Common Access Cards (CAC) and user ID/passwords. Access to personal information is restricted to... categories: Theater Medical Data Store, AHLTA--Theater, and Theater Medical Information Program Cache... conduct of operations. Routine uses of records maintained in the system, including categories of users and...

  2. 77 FR 22632 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-04-16

    ... authorized users by keyboard entry of unique passwords, access, and verify codes. RETENTION AND DISPOSAL... reflect that the SCIDO-R will also store outcome measures of impairment, and a registrant may have... program to accomplish this purpose. The Report of Intent to Amend a System of Records Notice and an...

  3. 75 FR 21253 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-23

    ... Issue Facility (CIF): Property book offices and Central Issue Facilities' supply rooms at most Army..., education information, Army Knowledge Online (AKO)user id, living or deceased, military/civilian/foreign... Marketing Office. System manager(s) and address: Delete entry and replace with ``Project Director...

  4. 75 FR 57458 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-09-21

    ... the Armed Forces, Information Resources Management College, Joint Forces Staff College, National War..., International Student Management Office, Joint Reserve Affairs Center, NATO Education Center, Secretary of... education, and academic data.'' Authority for maintenance of the system: Delete entry and replace with ``10...

  5. 76 FR 61680 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-10-05

    ...-SWM-ZA), 5500 21st Street, Building 247, Fort Belvoir, VA 22060-5934. Commandant, U.S. Army War... telephone number, medical, employment and education information, gender, work e-mail address, personal e...): Standardized Student Records System purpose is to obtain training, education, experiential learning, personal...

  6. 76 FR 72391 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-23

    ... Centers for Disease Control and the National Institutes of Mental Health, DHHS, for the purpose of... and the National Institutes of Mental Health, DHHS, for the purpose of conducting studies concerned... records in the system: Storage: Electronic storage media. Retrievability: Retrieved by name, Social...

  7. 78 FR 12423 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-02-22

    ... comment period, comments may be viewed online through the Federal Docket Management System (FDMS) at www... about which the complaint was made, including name, Social Security number, account numbers, address... Litigation--To disclose information to the Department of Justice, or in a proceeding before a court...

  8. 75 FR 24932 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-05-06

    ... media. RETRIEVABILITY: Name, Social Security Number (SSN), biometric template, (fingerprints, face and... Security and Governmental Affairs, and the Office of Management and Budget (OMB) pursuant to paragraph 4c... controlled facility. CATEGORIES OF RECORDS IN THE SYSTEM: Name, Social Security Number (SSN); case number...

  9. 77 FR 26262 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-05-03

    ..., Social Security Number (SSN), gender, race, home address, family and dependent information, biometric... Senate Committee on Homeland Security and Governmental Affairs, and the Office of Management and Budget.... LDIA 0660 Security Files (July 24, 2006, 71 FR 41784) * * * * * Changes: System name: Delete entry and...

  10. 78 FR 35609 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-06-13

    ... Information Privacy Regulation; and E.O. 9397 (SSN), as amended.'' Purpose(s): Delete entry and replace with... PRIMS from the menu. Former service members who are seeking to determine whether this system of records... Password or CAC to gain access to site and then select PRIMS from the menu. Former service members seeking...

  11. 75 FR 77849 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-12-14

    ... Security Cooperation Agency (DSCA) Headquarters, and the Defense Media Activity. Categories of records in the system: For all employees: name, Social Security Number (SSN), e-mail address, supervisor's name...-diagnostic information concerning health readiness/restrictive duty, respirator usage and fit test results...

  12. 77 FR 32111 - Privacy Act System of Records

    Science.gov (United States)

    2012-05-31

    .... Stations have been required to maintain their public files at their main studios for decades, pursuant to...) proposes to add a new system of records, FCC/MB-2, ``Broadcast Station Public Inspection Files.'' The enactment of the Standardized and Enhanced Disclosure Requirements for Television Broadcast Licensee Public...

  13. 77 FR 46528 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2012-08-03

    ... Barcode. The second will strengthen the digital relationship with consumers and aid all customers with... services. * * * * * SYSTEM MANAGER(S) AND ADDRESS: [ADD NEW TEXT] President, Digital Solutions, United... States Postal Service, 475 L'Enfant Plaza SW., Washington, DC 20260. [CHANGE TO READ] Chief Marketing...

  14. 75 FR 22565 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-04-29

    ... Circular No. A-130, ``Federal Agency Responsibilities for Maintaining Records About Individuals,'' dated... and replace with ``Records in the system include name, address, sponsor and dependent's Social..., outpatient, and pharmacy utilization rates; budgetary and managerial cost accounting data, such as claims...

  15. 76 FR 51975 - Privacy Act System of Records

    Science.gov (United States)

    2011-08-19

    ... establish paternity, establishing and modifying orders of child support, identifying sources of income, and... system may be used to prepare information on items considered income for taxation purposes to be... source from which additional information is requested relevant to an FCC determination concerning an...

  16. 76 FR 48812 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-08-09

    ... investigation. Categories of records in the system: Individual's names, Social Security Number (SSN), address..., (Pub. L. 452), as amended; and E.O. 9397 (SSN), as amended. Purpose(s): Open and closed case listings... Security Number (SSN), or case control number. ] Safeguards: Computerized records maintained in a...

  17. 76 FR 72691 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-25

    ... their full name, Social Security Number (SSN), current address, telephone number, and office or... Security Number (SSN), current address, telephone number, and office or organization where currently... of records in the system: Individual's name, Social Security Number (SSN), activity code, home...

  18. 75 FR 81249 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-12-27

    ... of records in the system: Individual's name, Social Security Number (SSN), date of birth, home... Corps Order 11000.11B, Marine Corps Fire Protection and Emergency Services Program; and E.O. 9397 (SSN... records in file folders. Retrievability: By name, Social Security Number (SSN), and/or date of birth...

  19. 78 FR 73516 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-12-06

    ... individual's name, Social Security Number (SSN), and case docket number and may include the individual's home... Regulation; and E.O. 9397 (SSN), as amended.'' * * * * * Routine uses of records maintained in the system...: Delete entry and replace with ``Name, case docket number, and/or SSN.'' * * * * * Notification procedure...

  20. 76 FR 26712 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-05-09

    ... (SSN), as amended. 5. Probable or potential effects on the privacy of individuals: None. 6. Is the... records in the system: Delete entry and replace with ``File contains, name, Social Security Number (SSN....O. 9397 (SSN), as amended.'' Purpose: Delete entry and replace with ``To maintain a listing of...

  1. 76 FR 13992 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-15

    ... (SSN), date of birth, gender, date of rank, military branch, occupation, duty station, joint.... 9397 (SSN), as amended.'' Purpose(s): Delete entry and replace with ``To track military officers in... retrieved by name and/ or SSN.'' * * * * * System manager(s) and address: Delete entry and replace with...

  2. 76 FR 70425 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-11-14

    ... requests should contain the full name and Social Security Number (SSN) of the individual.'' * * * * * DWHS... (SSN) and sponsoring DoD office. Authority for maintenance of the system: 10 U.S.C. 113, Secretary of Defense; DoD Directive 5110.4, Washington Headquarters Services (WHS); and E.O. 9397 (SSN), as amended...

  3. 78 FR 14292 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-03-05

    ... ``Name, Social Security Number (SSN), Department of Defense Identification Number (DoD ID Number) and... hospitals; and E.O. 9397 (SSN), as amended.'' * * * * * Routine uses of records maintained in the system..., SSN and/or DoD ID Number.'' Safeguards: Delete entry and replace with ``Records are maintained in file...

  4. 75 FR 6005 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-02-05

    .... Categories of records in the system: Name, Social Security Numbers (SSN), rank/grade, service number, marital...: Individual's name, Social Security Number (SSN) and/or service number. Safeguards: Electronic records are... purposes, individuals should provide their full name, Social Security Number (SSN), record type (if known...

  5. 78 FR 5787 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-01-28

    ..., selection, notification and testing of DIA employees and applicants for employment.'' Routine uses of... individuals covered by the system: Delete entry and replace with ``Civilian employees and applicants for... relating to testing plans, procedures, selection/scheduling records, records relating to the collection and...

  6. 78 FR 69653 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-11-20

    ... frequently being the source of critical information need for intelligence analysis. In the United States this..., Foreign, Counterterrorism and Counter Intelligence Operation Records. System location: Primary location: Commander, Office of Naval Intelligence, 4251 Suitland, MD 20395-2000. Decentralized segments are located at...

  7. 78 FR 56926 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2013-09-16

    ... enforcement and intelligence data for the compilation, fusion, storage, and comprehensive analysis of drug... Organized Crime Intelligence and Operations Center System,'' JUSTICE/CRM-028, last published at 74 FR 26733... Organized Crime Intelligence and Operations Center (IOC-2) and its member agencies to significantly disrupt...

  8. 76 FR 14951 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2011-03-18

    ... Medical Examination, Report of Medical History, Narrative Summary, Armed Forces Health Longitudinal... name, Social Security Number (SSN), and date of birth.'' Authority for maintenance of the system... Evaluation Board packages, Report of Medical Examination, Report of Medical History, Narrative Summary, Armed...

  9. 75 FR 3899 - Privacy Act of 1974; System of Records

    Science.gov (United States)

    2010-01-25

    ... Intelligence College (NGC), individuals that apply for a job through e-Recruit, requests access to the network, or badge to an NGA facility. Categories of records in the system: Names, Social Security Number (SSN...) data; education; health and life insurance; thrift savings plan (TSP); occupation; new hire applicant...

  10. 75 FR 54915 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-Sensory System...

    Science.gov (United States)

    2010-09-09

    ... Production Act of 1993--Sensory System for Critical Infrastructure Defect Recognition, Visualization and... Cooperative Research and Production Act of 1993, 15 U.S.C. 4301 et seq. (the Act''), Sensory System for Critical Infrastructure Defect Recognition, Visualization and Failure Prediction ('Sensory System'') has...

  11. 77 FR 3455 - Privacy Act of 1974; System of Records-Migrant Education Bypass Program Student Database

    Science.gov (United States)

    2012-01-24

    ... DEPARTMENT OF EDUCATION Privacy Act of 1974; System of Records--Migrant Education Bypass Program...: Notice of a new system of records. SUMMARY: In accordance with the Privacy Act of 1974, as amended (Privacy Act), the Department of Education (Department) publishes this notice of a new system of records...

  12. 75 FR 69715 - Privacy Act of 1974: New System of Records

    Science.gov (United States)

    2010-11-15

    ... Doc No: 2010-28834] OFFICE OF PERSONNEL MANAGEMENT Privacy Act of 1974: New System of Records AGENCY: U.S. Office of Personnel Management (OPM). ACTION: Notice to extend comment period for a new system..., Health Claims Data Warehouse, until December 15, 2010. The initial notice for this system was published...

  13. 75 FR 78271 - Privacy Act of 1974: New System of Records

    Science.gov (United States)

    2010-12-15

    ... MANAGEMENT Privacy Act of 1974: New System of Records AGENCY: U.S. Office of Personnel Management (OPM). ACTION: Projected date for publishing a revised notice for a new system of records. SUMMARY: Based on the... system of records, OPM/Central-15, Health Claims Data Warehouse, OPM has determined that it will publish...

  14. 76 FR 76215 - Privacy Act; System of Records: State-78, Risk Analysis and Management Records

    Science.gov (United States)

    2011-12-06

    ... DEPARTMENT OF STATE [Public Notice 7709] Privacy Act; System of Records: State-78, Risk Analysis... a system of records, Risk Analysis and Management Records, State-78, pursuant to the provisions of... INFORMATION: The Department of State proposes that the new system will be ``Risk Analysis and Management...

  15. 77 FR 14507 - Privacy Act of 1974 System of Records Notice

    Science.gov (United States)

    2012-03-12

    ... Complaint and Reasonable Accommodation Files, from its inventory of record systems because the relevant records are covered by existing government-wide system notices. DATES: Effective upon publication. FOR... the overall streamlining and management of CFTC Privacy Act record systems. Accordingly, this notice...

  16. 75 FR 60468 - Privacy Act of 1974; Report of an Altered System of Records

    Science.gov (United States)

    2010-09-30

    ... disposal; and update the system manager contact information. DATES: HRSA filed an altered system report... HUMAN SERVICES Health Resources and Services Administration Privacy Act of 1974; Report of an Altered System of Records AGENCY: Department of Health and Human Services (HHS), Health Resources and Services...

  17. 75 FR 31440 - Privacy Act of 1974; Report of a New System of Records

    Science.gov (United States)

    2010-06-03

    ... HUMAN SERVICES Privacy Act of 1974; Report of a New System of Records AGENCY: Department of Health and Human Services (HHS). ACTION: Notice of a New System of Records. SUMMARY: In accordance with the...: Effective Dates: HHS filed a new system report with the Chair of the House Committee on Government Reform...

  18. 77 FR 5865 - Privacy Act; System of Records: State-59, Refugee Case Records

    Science.gov (United States)

    2012-02-06

    ... DEPARTMENT OF STATE [Public Notice 7787] Privacy Act; System of Records: State-59, Refugee Case... systems of records, Refugee Case Records, State-59 and Refugee Processing Center Records, State-60... and Budget Circular No. A-130, Appendix I. The consolidated system will be titled State-59, Refugee...

  19. 75 FR 56601 - Privacy Act of 1974: New System of Records

    Science.gov (United States)

    2010-09-16

    ... OFFICE OF PERSONNEL MANAGEMENT Privacy Act of 1974: New System of Records AGENCY: U.S. Office of Personnel Management (OPM). ACTION: Notice of a new system of records. SUMMARY: The Patient Protection and... process that will be administered by the Office of Personnel Management (OPM). The system of records will...

  20. 78 FR 55284 - Privacy Act of 1974, as Amended; Notice of a New System of Records

    Science.gov (United States)

    2013-09-10

    ..., Application Management Branch, Finance & Procurement Systems Division, U.S. Department of the Interior... The Privacy Act of 1974, as amended, embodies fair information practice principles in a statutory... NAME: Oracle Federal Financials (OFF). SYSTEM CLASSIFICATION: Unclassified. SYSTEM LOCATION: 1. Finance...