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Sample records for synthesis characterization antibacterial

  1. SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ...

    African Journals Online (AJOL)

    Preferred Customer

    E-mail: a-ahmadi@kiau.ac.ir; ahmadikiau@yahoo.com. SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL EVALUATION OF. SOME NOVEL DERIVATIVES OF 2-BROMOMETHYL-BENZIMIDAZOLE. Abbas Ahmadi*. Department of Medicinal Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University,.

  2. Synthesis, Characterization and Antibacterial Activity of Imidazole ...

    African Journals Online (AJOL)

    NICO

    Synthesis, Characterization and Antibacterial Activity of. Imidazole Derivatives of 1,10-Phenanthroline and their .... Synthesis of Ligands (L1, L2). Ligands (L1. , L2) were synthesized by a method similar to one ... (50 mL). Dropwise addition of concentrated aqueaus ammonia to neutralize gave a yellow precipitate, which was ...

  3. Synthesis, characterization and antibacterial evaluation of ...

    African Journals Online (AJOL)

    The synthesis, characterization and anti-bacterial evaluation of two palmitoyl amino acids is reported in this work. The reported antimicrobial activity of some fatty acid derivatives encouraged the investigation of the possible influence of an aromatic group substituent on a saturated fatty acid residue. The compounds were ...

  4. Synthesis, Characterization and Antibacterial Evaluations of the ...

    African Journals Online (AJOL)

    MBI

    2014-06-05

    Jun 5, 2014 ... 39. Synthesis, Characterization and Antibacterial Evaluations of the Schiff. Base 2-(1-(2-(Piperazin-1-yl)ethylimino)ethyl)Phenol and its Complexes of. Mn(II), Ni(II) and Zn(II). Salga, M. S., Sada, I. and Abdullahi, A. Department of Pure and Industrial Chemistry, Umaru Musa 'Yar Adua University, Katsina.

  5. synthesis, physical characterization, antibacterial activity

    African Journals Online (AJOL)

    KEY WORDS: Cobalt (III) Schiff base complex, Thermodynamic parameters, Amines, Antibacterial activity. INTRODUCTION. For a long time tetradentate Schiff base complexes of transition metal ions have attracted many researchers interest in the field of coordination chemistry [1-4]. Though a large number of papers have ...

  6. Synthesis, spectral characterization and in vitro antibacterial ...

    African Journals Online (AJOL)

    Shafqat Nadeem

    2015-12-17

    Dec 17, 2015 ... Petra/Osiris/Molinspiration analysis. Abstract The paper emphasizes on the synthesis of Palladium(II) iodide complexes containing based ligands. The new compounds .... The spectral conditions were as follows: 32 K data points,. 1.822 s acquisition time, 2.00 s pulse delay and 6.00 ls pulse width. The 13C ...

  7. synthesis, characterization and antibacterial studies of metal

    African Journals Online (AJOL)

    Preferred Customer

    sulfonamide (sulfadiazine) with some N-alkyl-N-phenyl dithiocarbamate have been synthesized and characterized by elemental analysis, conductivity measurements, UV-Vis and FTIR spectroscopy. The complexes are formulated as four coordinate MN2S2 species in which the metal ions are coordinated to one molecule of.

  8. Synthesis, characterization and antibacterial studies of metal ...

    African Journals Online (AJOL)

    Co(II), Cu(II), Pd(II) and Pt(II) complexes of 4-amino-N-(2-pyrimidinyl)benzene sulfonamide (sulfadiazine) with some N-alkyl-N-phenyl dithiocarbamate have been synthesized and characterized by elemental analysis, conductivity measurements, UV-Vis and FTIR spectroscopy. The complexes are formulated as four ...

  9. Synthesis, physical characterization, antibacterial activity and ...

    African Journals Online (AJOL)

    Some five-coordinated cobalt(III) complexes were synthesized and characterized using elemental analysis, 1H NMR and IR spectra. The formation constants and the thermodynamic parameters were measured spectrophotometrically for the 1:1 adduct formation of [Co(Chel)(PBu3)]ClO4.H2O where Chel = cd3OMesalen, ...

  10. Synthesis, characterization and antibacterial activity of new fluorescent chitosan derivatives

    Czech Academy of Sciences Publication Activity Database

    Přichystalová, H.; Almonasy, N.; Abdel-Mohsen, A. M.; Abdel-Rahman, R. M.; Fouda, M. M. G.; Vojtova, L.; Kobera, Libor; Spotz, Z.; Burgert, L.; Jancar, J.

    2014-01-01

    Roč. 65, April (2014), s. 234-240 ISSN 0141-8130 Institutional support: RVO:61389013 Keywords : chitosan derivatives * fluorescence * antibacterial activity Subject RIV: CD - Macromolecular Chemistry Impact factor: 2.858, year: 2014

  11. Synthesis, characterization and antibacterial activity of Fe(II) mixed ...

    African Journals Online (AJOL)

    . The electronic spectrum of iron(II) complex in DMSO was recorded, and its salient features are reported. The activity studies data indicate that the complex is more potent antibacterial agent than the ligands and ciprofloxacin drug against the ...

  12. Synthesis, characterization and in vitro antibacterial activity of novel ...

    Indian Academy of Sciences (India)

    The prepared compounds were screened for antibacterial activity against Escherichia coli (E. coli ATCC 25922) and Staphylococcus aureus (S. aureus ATCC 5213) as gram negative and positive respectively. Also, in silico physicochemical parameters of synthesized compounds were studied to predict absorption and ...

  13. Synthesis, characterization and antibacterial activity of polyaniline/Pt-Pd nanocomposite.

    Science.gov (United States)

    Boomi, Pandi; Prabu, Halliah Gurumallesh; Mathiyarasu, Jayaraman

    2014-01-24

    Pt colloid and Pt-Pd colloid, pristine polyaniline, polyaniline/Pt nanocomposite and polyaniline/Pt-Pd nanocomposite were synthesized by simple chemical method. They were characterized by UV-Vis, FT-IR, XRD, TGA, SEM, HR-SEM and HR-TEM with EDAX techniques. The results proved that there is a strong interaction between metal nanoparticles (Pt-Pd) and polyaniline chains. This interaction creates changes in the backbone chain of polyaniline/Pt-Pd nanocomposite when compared to pristine polyaniline. The synthesized materials were evaluated for antibacterial activity, minimal inhibitory concentration and minimal bactericidal concentration. The results indicated that the nanocomposites exhibited improved antibacterial activity when compared to pristine polyaniline and individual metal colloids. This is the first report on the chemical synthesis of polyaniline/Pt-Pd nanocomposite, which exhibits antibacterial activity at micro molar concentration levels. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  14. A biotemplated nickel nanostructure: Synthesis, characterization and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Ashtari, Khadijeh [Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran (Iran, Islamic Republic of); Fasihi, Javad [Department of Analytical Chemistry, Faculty of Science, Tarbiat Modares University, Tehran (Iran, Islamic Republic of); Mollania, Nasrin [Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, P.O. Box 14115-175, Tehran (Iran, Islamic Republic of); Khajeh, Khosro, E-mail: khajeh@modares.ac.ir [Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran (Iran, Islamic Republic of); Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, P.O. Box 14115-175, Tehran (Iran, Islamic Republic of)

    2014-02-01

    Highlights: • Nickel nanostructure-encapsulated bacteria were prepared using electroless deposition. • Bacterium surface was activated by red-ox reaction of its surface amino acids. • Interfacial changes at cell surfaces were investigated using fluorescence spectroscopy. • TEM and AFM depicted morphological changes. • Antibacterial activity of nanostructure was examined against different bacteria strains. - Abstract: Nickel nanostructure-encapsulated bacteria were prepared using the electroless deposition procedure and activation of bacterium cell surface by red-ox reaction of surface amino acids. The electroless deposition step occurred in the presence of Ni(II) and dimethyl amine boran (DMAB). Interfacial changes at bacteria cell surfaces during the coating process were investigated using fluorescence spectroscopy. Fluorescence of tryptophan residues was completely quenched after the deposition of nickel onto bacteria surfaces. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) depicted morphological changes on the surface of the bacterium. It was found that the Ni coated nanostructure was mechanically stable after ultrasonication for 20 min. Significant increase in surface roughness of bacteria was also observed after deposition of Ni clusters. The amount of coated Ni on the bacteria surface was calculated as 36% w/w. The antibacterial activity of fabricated nanostructure in culture media was examined against three different bacteria strains; Escherichia coli, Bacillus subtilis and Xantomonas campestris. The minimum inhibitory concentrations (MIC) were determined as 500 mg/L, 350 mg/L and 200 mg/L against bacteria, respectively.

  15. Synthesis, Spectral Characterization, and Antibacterial and Antifungal Studies of PANI/V2O5 Nanocomposites

    Directory of Open Access Journals (Sweden)

    Chakradhar Sridhar

    2016-01-01

    Full Text Available The present study deals with the synthesis and characterization of nanocrystalline vanadium pentoxide (V2O5 nanoparticles and their antibacterial and antifungal activity on Staphylococcus aureus and Aspergillus niger, respectively, by agar diffusion method. The metal oxide has been synthesized by employing the sol-gel method, polyaniline (PANI has been synthesized by chemical oxidation, and PANI/V2O5 composites have been synthesized by in situ polymerization method with different ratios (10, 20, 30, 40, and 50 weight% of V2O5 in PANI. The newly prepared composites were characterized by FTIR and powder X-ray diffraction (P-XRD techniques and are found to be formed of PANI/V2O5 nanocomposites, and also the compounds showed moderate antibacterial and antifungal activity.

  16. Synthesis, Characterization and Antibacterial Activity of New 1,2 ...

    African Journals Online (AJOL)

    Synthesis of two series of 1,2- and 1,4-bis(thioureido)benzene derivatives was accomplished by the treatment of corresponding alkanoyl/aroyl chlorides with potassium thiocyanate in dry acetone to afford the respective isothiocyanates as intermediates. The latter were treated in situ with 1,2- and 1,4-diaminobenzene, ...

  17. Synthesis, characterization and in vitro antibacterial activity of novel ...

    Indian Academy of Sciences (India)

    MARZIEH ABBASI

    dried and activated in vacuum at 100. ◦. C. Activated diatomite. (2 g) was dispersed in dry CH2Cl2 and chlorosulfonic acid. (1 mL) was added to the solution at room temperature. After. 2 h, the white solid was filtered, washed repeatedly by dry. CH2Cl2, and dried at 120. ◦. C for 12 h. 2.3 General procedure for the synthesis of.

  18. Graphene oxide-modified ZnO particles: synthesis, characterization, and antibacterial properties

    Directory of Open Access Journals (Sweden)

    Zhong LL

    2015-08-01

    Full Text Available Linlin Zhong, Kyusik Yun Department of Bionanotechnology, Gachon University, Gyeonggi-do, Republic of Korea Abstract: Nanosized ZnO particles with diameters of 15 nm were prepared with a solution precipitation method at low cost and high yield. The synthesis of the particles was functionalized by the organic solvent dimethylformamide, and the particles were covalently bonded to the surface of graphene oxide. The morphology of the graphene oxide sheets and ZnO particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy. Fourier transform infrared spectroscopy and X-ray diffraction were used to analyze the physical and chemical properties of the ZnO/graphene oxide composites that differed from those of the individual components. Enhanced electrochemical properties were detected with cyclic voltammetry, with a redox peak of the composites at 0.025 mV. Excellent antibacterial activity of ZnO/graphene oxide composites was observed with a microdilution method in which minimum inhibitory concentrations of 6.25 µg/mL for Escherichia coli and Salmonella typhimurium, 12.5 µg/mL for Bacillus subtilis, and 25 µg/mL for Enterococcus faecalis. After further study of the antibacterial mechanism, we concluded that a vast number of reactive oxygen species formed on the surface of composites, improving antibacterial properties. Keywords: graphene oxide, ZnO, characterization, antibacterial property

  19. Synthesis, characterization and antibacterial screening of 2,4 ...

    African Journals Online (AJOL)

    seafood

    2012-05-15

    May 15, 2012 ... Air stable silver Ag(I) complexes of pyrimethamine and trimethoprim drugs have been synthesized and characterized by elemental analysis, Fourier transform infrared (FTIR) and ultraviolet visible (UV-Vis) spectroscopy, and conductivity measurement. The metal complexes formed a three and four ...

  20. Synthesis, characterization and antibacterial screening of 2,4 ...

    African Journals Online (AJOL)

    Air stable silver Ag(I) complexes of pyrimethamine and trimethoprim drugs have been synthesized and characterized by elemental analysis, Fourier transform infrared (FTIR) and ultraviolet visible (UV-Vis) spectroscopy, and conductivity measurement. The metal complexes formed a three and four coordinate geometry with ...

  1. Synthesis and characterization and antibacterial activity of some ...

    African Journals Online (AJOL)

    Some new transition metal complexes of ciprofloxacin-imine derived from ciprofloxacin and o-phenylenediamine were synthesized and characterized on the basis of melting point, magnetic moment, conductance measurements, elemental analysis, infrared, UV/Vis, nuclear magnetic resonance spectroscopy, mass spectra ...

  2. Synthesis, characterization and antibacterial activity of aspirin and ...

    African Journals Online (AJOL)

    Dr J. T. Ekanem

    Novel complexes of Co (11), Ni (11) and Fe (111) with aspirin and paracetamol have synthesized and characterized using infrared, electronic and Hnmr spectral, melting point and conductivity measurements. The two ligands have been found to act as bidentate chelating agents. Aspirin complexes coordinate through the ...

  3. Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

    Directory of Open Access Journals (Sweden)

    Long-Biao Guo

    2013-05-01

    Full Text Available This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR band at 1559 cm−1, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.

  4. Biogenic synthesis of silver nanoparticles from white rot fungi: Their characterization and antibacterial studies

    Directory of Open Access Journals (Sweden)

    Krishna Gudikandula

    2017-01-01

    Full Text Available The advanced research and development of silver nanoparticles (AgNPs is vast due to their incredible applications today. In this work, research was focused on the production, characterization and antibacterial studies of silver nanoparticles (AgNPs. We used an environmentally friendly extracellular biosynthetic method for the production of the AgNPs. The reducing agents used to produce the nanoparticles were from culture filtrate extracts made from two white rot fungi. Synthesis of colloidal AgNPs was monitored by UV-Visible spectroscopy. The UV-Visible spectrum showed a peak between 419 and 421 nm corresponding to the Plasmon absorbance of the AgNPs. The characterization of the AgNPs such as their size and shape was performed by Transmission Electron Microscopy (TEM techniques which indicated a size range of 15–25 nm. The anti-bacterial activity of AgNPs was investigated by using four Gram-negative and four Gram-positive bacteria. All the bacterial strains are showing good antibacterial activity.

  5. Herbal Plant Synthesis of Antibacterial Silver Nanoparticles by Solanum trilobatum and Its Characterization

    Directory of Open Access Journals (Sweden)

    M. Vanaja

    2014-01-01

    Full Text Available Green synthesis method of nanomaterials is rapidly growing in the nanotechnology field; it replaces the use of toxic chemicals and time consumption. In this present investigation we report the green synthesis of silver nanoparticles (AgNPs by using the leaf extract of medicinally valuable plant Solanum trilobatum. The influence of physical and chemical parameters on the silver nanoparticle fabrication such as incubation time, silver nitrate concentration, pH, and temperature is also studied in this present context. The green synthesized silver nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD, scanning electron microscope (SEM, energy dispersive X-ray (EDX, and transmission electron microscope (TEM. The SEM and TEM confirm the synthesis of spherical shape of nanocrystalline particles with the size range of 2–10 nm. FTIR reveals that the carboxyl and amine groups may be involved in the reduction of silver ions to silver nanoparticles. Antibacterial activity of synthesized silver nanoparticles was done by agar well diffusion method against different pathogenic bacteria. The green synthesized silver nanoparticles can be used in the field of medicine, due to their high antibacterial activity.

  6. Synthesis, characterization and antibacterial effect of new magnetically core–shell nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Allafchian, Alireza, E-mail: Allafchian@cc.iut.ac.ir [Nanotechnology and Advanced Materials Institute, Isfahan University of Technology, Isfahan 84156 83111 (Iran, Islamic Republic of); Bahramian, Hamid [Department of physics, Isfahan University of Technology, Isfahan 84156 83111 (Iran, Islamic Republic of); Hossein Jalali, Seyed Amir [Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156 83111 (Iran, Islamic Republic of); Ahmadvand, Hossein [Department of physics, Isfahan University of Technology, Isfahan 84156 83111 (Iran, Islamic Republic of)

    2015-11-15

    A new magnetically responsive three-component nanocomposite consisting of NiFe{sub 2}O{sub 4}, Poly Acrylonitrile Co Maleic Anhydride (PAMA) and nanosilver was synthesized and characterized and then its antibacterial activities were tested. For the preparation of NiFe{sub 2}O{sub 4}@Ag, NiFe{sub 2}O{sub 4} was coated by Ag and for the synthesis of NiFe{sub 2}O{sub 4}@PAMA@Ag, NiFe{sub 2}O{sub 4} was first covered by PAMA and then silver nanoparticles were immobilized on the surface of the PAMA shell. The nanocomposites were studied using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). The antibacterial activity of the synthesized nanocomposite against some gram positive and gram negative bacteria was studied and compared with that of naked NiFe{sub 2}O{sub 4}, NiFe{sub 2}O{sub 4}@Ag and NiFe{sub 2}O{sub 4}@PAMA. The NiFe{sub 2}O{sub 4}@PAMA@Ag had better antibacterial activity and could be readily isolated from the aqueous solution via magnetic decantation, thereby avoiding the contamination of the environment. - Highlights: • A novel NiFe{sub 2}O{sub 4}@PAMA@Ag nanocomposites magnetic composite has been prepared. • This system display potent antimicrobial activity toward some bacterial species. • The antibacterial effect was studied by disk diffusion method. • This composite can be easily removed from solution by magnetic decantation.

  7. Synthesis and antibacterial characterization of sustainable nanosilver using naturally-derived macromolecules

    Energy Technology Data Exchange (ETDEWEB)

    Osonga, Francis J.; Kariuki, Victor M.; Yazgan, Idris; Jimenez, Apryl; Luther, David; Schulte, Jürgen; Sadik, Omowunmi A., E-mail: osadik@binghamton.edu

    2016-09-01

    Greener nanosynthesis utilizes fewer amounts of materials, water, and energy; while reducing or replacing the need for organic solvents. A novel approach is presented using naturally-derived flavonoids including Quercetin pentaphosphate (QPP), Quercetin sulfonic acid (QSA) and Apigenin Triphosphate (ATRP). These water soluble, phosphorylated flavonoids were utilized both as reducing agent and stabilizer. The synthesis was achieved at room temperature using water as a solvent and it requires no capping agents. The efficiency of the resulting silver nanoparticle synthesis was compared with naturally-occurring flavonoid such as Quercetin (QCR). Results show that QCR reduced Ag{sup +} faster followed by QPP, QSA and ATRP respectively. This is the first evidence of direct utilization of QCR for synthesis of silver nanoparticles (AgNPs) in water. The percentage conversion of Ag{sup +} to Ag{sup 0} was determined to be 96% after 35 min. The synthesized nanoparticles were characterized using Transmission electron microscopy (TEM), Energy dispersive absorption spectroscopy (EDS), UV–vis spectroscopy, High resolution TEM (HR-TEM) with selected area electron diffraction (SAED). The particle sizes ranged from 2 to 80 nm with an average size of 22 nm and in the case of ATRP, the nanoparticle shapes varied from spherical to hexagonal with dispersed particle size ranging from 2 to 30 nm. Crystallinity was confirmed by XRD and the SAED of (111), (200), and the fringes observed in HRTEM images. Results were in agreement with the UV resonance peaks of 369–440 nm. The particles also exhibit excellent antibacterial activity against Staphylococcus epidermidis, Escherichia coli and Citrobacter freundii in water. - Highlights: • Greener nanosilver prepared using flavonoid derivatives • Synthesized nanosilver exhibits exhibit antibacterial activity. • Approach suitable for industrial synthesis.

  8. Green synthesis, characterization and antibacterial efficacy of palladium nanoparticles synthesized using Filicium decipiens leaf extract

    Science.gov (United States)

    Sharmila, G.; Farzana Fathima, M.; Haries, S.; Geetha, S.; Manoj Kumar, N.; Muthukumaran, C.

    2017-06-01

    Synthesis of metal nanoparticles through green chemistry route is an emerging eco-friendly approach in the present days. An eco-friendly, biogenic synthesis of palladium nanoparticles (PdNPs) using Filicium decipiens leaf extract was reported in the present study. The synthesized PdNPs were characterized by UV-visible spectroscopy, Transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The PdNPs formation was confirmed by UV-visible spectrophotometer and spherical shaped PdNPs with size range of 2-22 nm was observed in TEM analysis. Energy dispersive X-ray spectroscopy (EDS) analysis confirmed the presence of palladium in the synthesized nanoparticles. The crystalline nature of PdNPs was confirmed by XRD pattern and compared with the standard. The phytochemicals and proteins were identified by their functional groups in FT-IR spectrum and revealed the amide, amine groups present in F. decipiens may have involved in the bio-reduction reaction for PdNPs synthesis. Prepared PdNPs showed potential antibacterial activity against both Gram-positive and Gram-negative bacteria. F. decipiens leaf extract based PdNPs showed high bactericidal activity against Escherichia coli, Pseudomonas aeruginosa as compared to Staphylococcus aureus and Bacillus subtilis Results showed that phytochemicals rich F. decipiens leaf extract may be utilized as an effective non-toxic reducing agent for PdNPs synthesis and prepared PdNPs may useful in biomedical applications.

  9. Synthesis and characterization of Ag nanoparticles decorated mesoporous sintered activated carbon with antibacterial and adsorptive properties

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Wenxia; Xiao, Kaijun, E-mail: fekjxiao@scut.edu.cn; He, Tinglin; Zhu, Liang, E-mail: zhuliang@scut.edu.cn

    2015-10-25

    In this study, the sliver nanoparticles (AgNPs) immobilized on the sintered activated carbon (Ag/SAC) were synthesized by the ultrasonic-assisted impregnation method and were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD) and nitrogen adsorption. SEM showed that the AgNPs were well embedded in the SAC and immersion time had an important influence on final morphologies of AgNPs. Longer immersing duration caused significant aggregation of the AgNPs. The XRD data revealed that the successful synthesis of AgNPs on the SAC and immobilizing AgNPs on sintered active carbon did not change the crystalline degree of SAC. Texture characteristics were determined by analysis of the N{sub 2}/77 K isotherms. The minimum inhibitory concentration (MIC) of Ag/SAC against Escherichia coli (DH5α) and Staphyloccocus aureus (ATCC 29213) was evaluated by a broth dilution method. MICs such as 5 mg/L (against E. coli) and 10 mg/L (against S. aureus) suggest that Ag/SAC have predominant antibacterial activity compared to active carbon. - Highlights: • Sintered active carbon (SAC) was coated with Ag via a facile approach. • The Ag/SAC exhibit good adsorption properties and excellent antibacterial effects. • The Ag/SAC was durable and stable in the application of water purification.

  10. Synthesis and characterization of Ag nanoparticles decorated mesoporous sintered activated carbon with antibacterial and adsorptive properties

    International Nuclear Information System (INIS)

    Wang, Wenxia; Xiao, Kaijun; He, Tinglin; Zhu, Liang

    2015-01-01

    In this study, the sliver nanoparticles (AgNPs) immobilized on the sintered activated carbon (Ag/SAC) were synthesized by the ultrasonic-assisted impregnation method and were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD) and nitrogen adsorption. SEM showed that the AgNPs were well embedded in the SAC and immersion time had an important influence on final morphologies of AgNPs. Longer immersing duration caused significant aggregation of the AgNPs. The XRD data revealed that the successful synthesis of AgNPs on the SAC and immobilizing AgNPs on sintered active carbon did not change the crystalline degree of SAC. Texture characteristics were determined by analysis of the N 2 /77 K isotherms. The minimum inhibitory concentration (MIC) of Ag/SAC against Escherichia coli (DH5α) and Staphyloccocus aureus (ATCC 29213) was evaluated by a broth dilution method. MICs such as 5 mg/L (against E. coli) and 10 mg/L (against S. aureus) suggest that Ag/SAC have predominant antibacterial activity compared to active carbon. - Highlights: • Sintered active carbon (SAC) was coated with Ag via a facile approach. • The Ag/SAC exhibit good adsorption properties and excellent antibacterial effects. • The Ag/SAC was durable and stable in the application of water purification

  11. Synthesis and characterization of polyaniline/Ag-Pt nanocomposite for improved antibacterial activity.

    Science.gov (United States)

    Boomi, Pandi; Prabu, Halliah Gurumallesh; Mathiyarasu, Jayaraman

    2013-03-01

    Polyaniline, polyaniline/Ag-Pt nanocomposite and bimetal (Ag-Pt) colloidal solution were chemically synthesized and characterized by UV-vis, XRD, FT-IR, TGA, HRSEM with EDAX and HRTEM techniques. The results reveal that there was a strong interaction between Ag-Pt nanocomposite and polyaniline chains. This interaction makes only small changes in the backbone chain of polyaniline/Ag-Pt nanocomposite when compared with polyaniline. TGA result revealed greater thermal stability of the composite. HRSEM images showed pebble like morphology for polyaniline/Ag-Pt nanocomposite. The average grain size of Ag-Pt nanoparticle was found to be 2-5 nm, which is confirmed by HRTEM analysis. The polyaniline and polyaniline/Ag-Pt nanocomposite were tested for antibacterial activity. The composite showed improved inhibition efficiency with a maximum zone diameter of 30 ± 1.25 mm against Staphylococcus aureus. This is the first report on the synthesis and its antibacterial activity of polyaniline/Ag-Pt nanocomposite. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Synthesis and characterization of novel silver nanoparticles using Chamaemelum nobile extract for antibacterial application

    Science.gov (United States)

    Erjaee, Hoda; Rajaian, Hamid; Nazifi, Saeed

    2017-06-01

    The present study reports green synthesis of silver nanoparticles (AgNPs) at room temperature using aqueous Chamaemelum nobile extract for the first time. The effect of silver nitrate concentration, quantity of the plant extract and the reaction time on particle size was optimized and studied by UV-Vis spectroscopy and dynamic light scattering. The appearance of brownish color with λ max of 422 nm confirmed the formation of AgNPs. Synthesized nanoparticles were further characterized by Fourier transform infrared spectroscopy, x-ray diffraction and transmission electron microscopy. In addition, antimicrobial activity of the AgNPs against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Bacillus subtilis was evaluated based on the inhibition zone using the disc-diffusion assay and measurement of minimal inhibition concentration and minimal bactericidal concentration by standard microdilution method. In conclusion, synthesis of nanoparticle with aqueous Chamaemelum nobile extract is simple, rapid, environmentally benign and inexpensive. Moreover, these synthesized nanoparticles exhibit significant antibacterial activity.

  13. Synthesis, characterization and antibacterial study on the chitosan-functionalized Ag nanoparticles.

    Science.gov (United States)

    Biao, Linhai; Tan, Shengnan; Wang, Yuanlin; Guo, Ximin; Fu, Yujie; Xu, Fengjie; Zu, Yuangang; Liu, Zhiguo

    2017-07-01

    This study provided a facile, one-step hydrothermal method to synthesize stable Ag colloid in aqueous solution by utilizing chitosan as both reductant and stabilizer. The formation of chitosan-functionalized Ag nanoparticles was verified by UV-Vis, FTIR, TEM, AFM and XRD measurements. FTIR results revealed that the primary amine groups and amide groups of chitosan have specific interactions with the surface of Ag nanoparticles. The average diameter of the Ag nanoparticles is 10.0±5.4nm as determined by TEM. Ag nanoparticles are highly crystalline as revealed by HR-TEM and XRD measurements. The size and shape of Ag nanoparticles are also found to depend on the pH condition in the synthesis. Ag nanoparticles were the main products at pH5.0 whereas large Ag nanotriangle and truncated triangular nanoplate were dominant at pH4.0 in the synthesis. Due to its monodispersity and good stability, the chitosan-functionalized Ag colloid synthesized at pH5.0 was further tested for its antibacterial activities against gram-positive bacteria, gram-negative bacteria and fungus. The results of zone of inhibition, inhibition ratio and SEM characterization revealed that chitosan-functionalized Ag nanoparticles have great bactericidal efficiency against both bacteria and fungus. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Quaternized Carboxymethyl Chitosan-Based Silver Nanoparticles Hybrid: Microwave-Assisted Synthesis, Characterization and Antibacterial Activity

    Directory of Open Access Journals (Sweden)

    Siqi Huang

    2016-06-01

    Full Text Available A facile, efficient, and eco-friendly approach for the preparation of uniform silver nanoparticles (Ag NPs was developed. The synthesis was conducted in an aqueous medium exposed to microwave irradiation for 8 min, using laboratory-prepared, water-soluble quaternized carboxymethyl chitosan (QCMC as a chemical reducer and stabilizer and silver nitrate as the silver source. The structure of the prepared QCMC was characterized using Fourier transform infrared (FT-IR and 1H nuclear magnetic resonance (NMR. The formation, size distribution, and dispersion of the Ag NPs in the QCMC matrix were determined using X-ray diffraction (XRD, X-ray photoelectron spectroscopy (XPS, ultraviolet-visible (UV-Vis, transmission electron microscopy (TEM, and field emission scanning electron microscope (FESEM analysis, and the thermal stability and antibacterial properties of the synthesized QCMC-based Ag NPs composite (QCMC-Ag were also explored. The results revealed that (1 QCMC was successfully prepared by grafting quaternary ammonium groups onto carboxymethyl chitosan (CMC chains under microwave irradiation in water for 90 min and this substitution appeared to have occurred at -NH2 sites on C2 position of the pyranoid ring; (2 uniform and stable spherical Ag NPs could be synthesized when QCMC was used as the reducing and stabilizing agent; (3 Ag NPs were well dispersed in the QCMC matrix with a narrow size distribiution in the range of 17–31 nm without aggregation; and (4 due to the presence of Ag NPs, the thermal stability and antibacterial activity of QCMC-Ag were dramatically improved relative to QCMC.

  15. Synthesis, characterization, controlled release, and antibacterial studies of a novel streptomycin chitosan magnetic nanoantibiotic

    Directory of Open Access Journals (Sweden)

    Hussein-Al-Ali SH

    2014-01-01

    Full Text Available Samer Hasan Hussein-Al-Ali,1 Mohamed Ezzat El Zowalaty,2,6 Mohd Zobir Hussein,3 Maznah Ismail,1,4 Thomas J Webster,5,7 1Laboratory of Molecular Biomedicine, 2Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, 3Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, 4Department of Nutrition and Dietetics, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Selangor, Malaysia; 5Department of Chemical Engineering and Program in Bioengineering, Northeastern University, Boston, MA, USA; 6Department of Environmental Health, Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, 7Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: This study describes the preparation, characterization, and controlled release of a streptomycin-chitosan-magnetic nanoparticle-based antibiotic in an effort to improve the treatment of bacterial infections. Specifically, chitosan-magnetic nanoparticles were synthesized by an incorporation method and were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and vibrating sample magnetometry. Streptomycin was incorporated into the nanoparticles to form a streptomycin-coated chitosan-magnetic nanoparticle (Strep-CS-MNP nanocomposite. The release profiles showed an initially fast release, which became slower as time progressed. The percentage of drug released after 350 minutes was around 100%, and the best fit mathematical model for drug release was the pseudo-second order model. The Strep-CS-MNP nanocomposite showed enhanced antibacterial activity against methicillin-resistant Staphylococcus aureus. This study forms a significant basis for further investigation of the Strep-CS-MNP nanocomposite in the treatment of various bacterial infections. Keywords: magnetic nanoparticles, streptomycin, nanoantibiotics, chitosan, release

  16. Green Synthesis of Silver Nanoparticles, Their Characterization, Application and Antibacterial Activity †

    Science.gov (United States)

    Okafor, Florence; Janen, Afef; Kukhtareva, Tatiana; Edwards, Vernessa; Curley, Michael

    2013-01-01

    Our research focused on the production, characterization and application of silver nanoparticles (AgNPs), which can be utilized in biomedical research and environmental cleaning applications. We used an environmentally friendly extracellular biosynthetic technique for the production of the AgNPs. The reducing agents used to produce the nanoparticles were from aqueous extracts made from the leaves of various plants. Synthesis of colloidal AgNPs was monitored by UV-Visible spectroscopy. The UV-Visible spectrum showed a peak between 417 and 425 nm corresponding to the Plasmon absorbance of the AgNPs. The characterization of the AgNPs such as their size and shape was performed by Atom Force Microscopy (AFM), and Transmission Electron Microscopy (TEM) techniques which indicated a size range of 3 to 15 nm. The anti-bacterial activity of AgNPs was investigated at concentrations between 2 and 15 ppm for Gram-negative and Gram-positive bacteria. Staphylococcus aureus and Kocuria rhizophila, Bacillus thuringiensis (Gram-positive organisms); Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhimurium (Gram-negative organisms) were exposed to AgNPs using Bioscreen C. The results indicated that AgNPs at a concentration of 2 and 4 ppm, inhibited bacterial growth. Preliminary evaluation of cytotoxicity of biosynthesized silver nanoparticles was accomplished using the InQ™ Cell Research System instrument with HEK 293 cells. This investigation demonstrated that silver nanoparticles with a concentration of 2 ppm and 4 ppm were not toxic for human healthy cells, but inhibit bacterial growth. PMID:24157517

  17. Synthesis, characterization and antibacterial properties of dihydroxy quaternary ammonium salts with long chain alkyl bromides.

    Science.gov (United States)

    Liu, Wen-Shuai; Wang, Chun-Hua; Sun, Ju-Feng; Hou, Gui-Ge; Wang, Yu-Peng; Qu, Rong-Jun

    2015-01-01

    Five N-methyl-N-R-N,N-bis(2-hydroxyethyl) ammonium bromides (R = -benzyl (chloride, BNQAS), -dodecyl (C12QAS), -tetradecyl (C14QAS), -hexadecyl (C16QAS), -octadecyl (C18QAS)) were prepared based on N-methyldiethanolamine (MDEA) and halohydrocarbon. Five QAS were characterized by FTIR, NMR, and MS. BNQAS, C12QAS, C14QAS, and C16QAS were confirmed by X-ray single-crystal diffraction. Their antibacterial properties indicated good antibacterial abilities against E. coli, S. aureus, B. subtilis, especially C12QAS with the best antibacterial ability (100% to E. coli, 95.65% to S. aureus, and 91.41% to B. subtilis). In addition, C12QAS also displayed the best antifungal activities than BNQAS and C18QAS against Cytospora mandshurica, Botryosphaeria ribis, Physalospora piricola, and Glomerella cingulata with the ratio of full marks. The strategy provides a facile way to design and develop new types of antibacterial drugs for application in preventing the fruit rot, especially apple. © 2014 John Wiley & Sons A/S.

  18. Synthesis, characterization and antibacterial studies of ruthenium(III) complexes derived from chitosan schiff base.

    Science.gov (United States)

    Vadivel, T; Dhamodaran, M

    2016-09-01

    Chitosan can be modified chemically by condensation reaction of deacetylated chitosan with aldehyde in homogeneous phase. This condensation is carried by primary amine (NH2) with aldehyde (CHO) to form corresponding schiff base. The chitosan biopolymer schiff base derivatives are synthesized with substituted aldehydes namely 4-hydroxy-3-methoxy benzaldehyde, 2-hydroxy benzaldehyde, and 2-hydroxy-3-methoxy benzaldehyde, becomes a complexing agent or ligand. The Ruthenium(III) complexes were obtained by complexation of Ruthenium with schiff base ligands and this product exhibits as an excellent solubility and more biocompatibility. The novel series of schiff base Ruthenium(III) complexes are characterized by Elemental analysis, FT-IR spectroscopy, and Thermo-gravimetric analysis (TGA). The synthesized complexes have been subjected to antibacterial study. The antibacterial results indicated that the antibacterial activity of the complexes were more effective against Gram positive and Gram negative pathogenic bacteria. These findings are giving suitable support for developing new antibacterial agent and expand our scope for applications. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity

    Science.gov (United States)

    Anandalakshmi, K.; Venugobal, J.; Ramasamy, V.

    2016-03-01

    In this paper, an aqueous extract of fresh leaves of Pedalium murex was used for the synthesis of silver (Ag) nanoparticles. Different biological methods are gaining recognition for the production of silver nanoparticles (AgNPs) due to their multiple applications. The use of plants in the green synthesis of nanoparticles emerges as a cost-effective and eco-friendly approach. Characterization of nanoparticles was done using different methods, which include; ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR), powder X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), energy dispersive X-ray analysis (EDAX), fluorescence emission spectroscopy, transmission electron microscope (TEM), dynamic light scattering (DLS), zeta potential and antibacterial activity. UV-visible spectrum of the aqueous medium containing silver nanoparticles showed absorption peak at around 430 nm. Fourier transform infrared spectra had shown that the biomolecule compounds were responsible for the reduction and capping material of silver nanoparticles. XRD study showed the particles to be crystalline in nature, with a face-centered cubic (fcc) structure. The size and stability were detected using DLS and zeta potential analysis. The antibacterial activity of AgNPs against generally found bacteria was assessed to find their potential use in silver-containing antibacterial product.

  20. Green synthesis, characterization and antibacterial activities of silver nanoparticles from strawberry fruit extract

    Directory of Open Access Journals (Sweden)

    Umoren Saviour A.

    2017-12-01

    Full Text Available Silver nanoparticles (AgNPs have been synthesized in the presence of Strawberry fruit extract (SBFE at room temperature. The synthesized AgNPs was characterized by UV-vis spectroscopy, SEM, EDS, XRD, TEM and FTIR. The UV-vis spectra of the AgNPs show SPR band at 450 nm. TEM results indicate that AgNPs are spherical in shape and size range between 7–65 nm. Antibacterial activity of the synthesized AgNPs has been assessed against Pseudomonas aeruginosa and Bacillus licheniformis. The results show that AgNPs exhibit inhibitory effect and effect is a function of AgNPs concentration. The antibacterial activity of the prepared AgNPs has been compared with two antibiotics, amoxicillin and ciprofloxacin. It is found that the antibiotics perform better than AgNPs.

  1. Synthesis, spectroscopic characterization and antibacterial screening of novel Mannich bases of Ganciclovir

    Directory of Open Access Journals (Sweden)

    Sheela Joshi

    2017-02-01

    Full Text Available Biologically active Mannich bases with heteroaromatic ring system of substituted guanine derivative (2-amino-9 [{(1,3 di hydroxy propane-2yl oxy} methyl] 6-9 dihydro-3H-purine-6-one (ganciclovir, have been synthesized via Mannich reaction. The aminomethylation of ganciclovir with various biologically potent sulphonamides was carried out and then characterized by elemental analysis and spectral studies – UV, IR, 1H NMR, powder X-ray diffraction and Scanning Electron Microscopy. The compounds were screened for their antibacterial activity against various pathogenic bacteria at varying concentrations. The antibacterial activity of derived Mannich bases was compared with parent sulphonamides. The toxicity of synthesized Mannich bases was ascertained by LD50 test.

  2. Microwave-assisted synthesis, characterization and antibacterial activity of Ag/ZnO nanoparticles supported bentonite clay

    International Nuclear Information System (INIS)

    Motshekga, Sarah C.; Ray, Suprakas S.; Onyango, Maurice S.; Momba, Maggie N.B.

    2013-01-01

    Highlights: • A facile, fast and effective method was used for preparing metal–clay composites. • The metal/oxide loaded on clay exhibited narrow size range at nano scale. • The composites deactivate both Gram negative and Gram positive bacteria. • A combination of metal and metal oxide provides the best antibacterial property. -- Abstract: Composites of silver–zinc oxide nanoparticles supported on bentonite clay were synthesized by the microwave-assisted synthesis method for use as an antibacterial material. Silver nitrate was used as the precursor of silver nanoparticles while zinc oxide nanoparticles were commercially sourced. The composites were characterized by powder X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier transform infrared (FTIR) and BET surface area measurements. XRD spectra showed peaks of silver confirming the formation of the silver and not of the silver nitrate or any other impurity of the metal. Meanwhile TEM confirmed the formation of silver and zinc oxide nanoparticles on the clay layers, with particle sizes ranging from 9–30 nm and 15–70 nm, respectively. The antibacterial activities of the composites were evaluated against Gram negative Escherichia coli bacteria and Gram positive Enterococcus faecalis bacteria by the disc diffusion method. Whereas both composites of Ag-clay and ZnO-clay showed good antibacterial activity against bacteria, a better antibacterial activity was observed with Ag/ZnO-clay composite. The results therefore reveal that Ag/ZnO-clay composite is a promising bactericide that can be used for deactivating microbes in water

  3. Microwave-assisted synthesis, characterization and antibacterial activity of Ag/ZnO nanoparticles supported bentonite clay

    Energy Technology Data Exchange (ETDEWEB)

    Motshekga, Sarah C. [DST/CSIR National Centre for Nanostructured Materials, Council for Scientific and Industrial Research, Pretoria 0001 (South Africa); Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology, Pretoria 0001 (South Africa); Ray, Suprakas S. [DST/CSIR National Centre for Nanostructured Materials, Council for Scientific and Industrial Research, Pretoria 0001 (South Africa); Onyango, Maurice S., E-mail: OnyangoMS@tut.ac.za [Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology, Pretoria 0001 (South Africa); Momba, Maggie N.B. [Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Arcadia Campus, Pretoria 0001 (South Africa)

    2013-11-15

    Highlights: • A facile, fast and effective method was used for preparing metal–clay composites. • The metal/oxide loaded on clay exhibited narrow size range at nano scale. • The composites deactivate both Gram negative and Gram positive bacteria. • A combination of metal and metal oxide provides the best antibacterial property. -- Abstract: Composites of silver–zinc oxide nanoparticles supported on bentonite clay were synthesized by the microwave-assisted synthesis method for use as an antibacterial material. Silver nitrate was used as the precursor of silver nanoparticles while zinc oxide nanoparticles were commercially sourced. The composites were characterized by powder X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier transform infrared (FTIR) and BET surface area measurements. XRD spectra showed peaks of silver confirming the formation of the silver and not of the silver nitrate or any other impurity of the metal. Meanwhile TEM confirmed the formation of silver and zinc oxide nanoparticles on the clay layers, with particle sizes ranging from 9–30 nm and 15–70 nm, respectively. The antibacterial activities of the composites were evaluated against Gram negative Escherichia coli bacteria and Gram positive Enterococcus faecalis bacteria by the disc diffusion method. Whereas both composites of Ag-clay and ZnO-clay showed good antibacterial activity against bacteria, a better antibacterial activity was observed with Ag/ZnO-clay composite. The results therefore reveal that Ag/ZnO-clay composite is a promising bactericide that can be used for deactivating microbes in water.

  4. Synthesis, characterization, and controlled release antibacterial behavior of antibiotic intercalated Mg–Al layered double hydroxides

    International Nuclear Information System (INIS)

    Wang, Yi; Zhang, Dun

    2012-01-01

    Graphical abstract: The antibiotic anion released from Mg–Al LDHs provides a controlled release antibacterial activity against the growth of Micrococcus lysodeikticus in 3.5% NaCl solution. Highlights: ► Antibiotic anion intercalated LDHs were synthesized and characterized. ► The ion-exchange one is responsible for the release process. ► The diffusion through particle is the release rate limiting step. ► LDHs loaded with antibiotic anion have high antibacterial capabilities. -- Abstract: Antibiotic–inorganic clay composites including four antibiotic anions, namely, benzoate (BZ), succinate (SU), benzylpenicillin (BP), and ticarcillin (TC) anions, intercalated Mg–Al layered double hydroxides (LDHs) were synthesized via ion-exchange. Powder X-ray diffraction and Fourier transform infrared spectrum analyses showed the successful intercalation of antibiotic anion into the LDH interlayer. BZ and BP anions were accommodated in the interlayer region as a bilayer, whereas SU and TC anions were intercalated in a monolayer arrangement. Kinetic simulation of the release data indicated that ion-exchange was responsible for the release process, and the diffusion through the particles was the rate-limiting step. The antibacterial capabilities of LDHs loaded with antibiotic anion toward Micrococcus lysodeikticus growth were analyzed using a turbidimetric method. Significant high inhibition rate was observed when LDH nanohybrid was introduced in 3.5% NaCl solution. Therefore, this hybrid material may be applied as nanocontainer in active antifouling coating for marine equipment.

  5. Synthesis, characterization, and controlled release antibacterial behavior of antibiotic intercalated Mg–Al layered double hydroxides

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yi [Shandong Provincial Key Lab of Corrosion Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 (China); Zhang, Dun, E-mail: zhangdun@qdio.ac.cn [Shandong Provincial Key Lab of Corrosion Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 (China)

    2012-11-15

    Graphical abstract: The antibiotic anion released from Mg–Al LDHs provides a controlled release antibacterial activity against the growth of Micrococcus lysodeikticus in 3.5% NaCl solution. Highlights: ► Antibiotic anion intercalated LDHs were synthesized and characterized. ► The ion-exchange one is responsible for the release process. ► The diffusion through particle is the release rate limiting step. ► LDHs loaded with antibiotic anion have high antibacterial capabilities. -- Abstract: Antibiotic–inorganic clay composites including four antibiotic anions, namely, benzoate (BZ), succinate (SU), benzylpenicillin (BP), and ticarcillin (TC) anions, intercalated Mg–Al layered double hydroxides (LDHs) were synthesized via ion-exchange. Powder X-ray diffraction and Fourier transform infrared spectrum analyses showed the successful intercalation of antibiotic anion into the LDH interlayer. BZ and BP anions were accommodated in the interlayer region as a bilayer, whereas SU and TC anions were intercalated in a monolayer arrangement. Kinetic simulation of the release data indicated that ion-exchange was responsible for the release process, and the diffusion through the particles was the rate-limiting step. The antibacterial capabilities of LDHs loaded with antibiotic anion toward Micrococcus lysodeikticus growth were analyzed using a turbidimetric method. Significant high inhibition rate was observed when LDH nanohybrid was introduced in 3.5% NaCl solution. Therefore, this hybrid material may be applied as nanocontainer in active antifouling coating for marine equipment.

  6. Ursolic Acid Hydrazide Based Organometallic Complexes: Synthesis, Characterization, Antibacterial, Antioxidant, and Docking Studies

    Directory of Open Access Journals (Sweden)

    Muafia Jabeen

    2018-03-01

    Full Text Available In thecurrent research work,eleven metal complexes were synthesized from the hydrazide derivative of ursolic acid. Metal complexes of tin, antimony and iron were synthesized and characterized by FT-IR and NMR spectroscopy. The antibacterial and antioxidant activities were performed for these complexes, which revealed that the metal complexes synthesized are more potent than their parent compounds. We observed that antioxidant activity showed by triphenyltin complex was significant and least activity have been shown by antimony trichloride complex. The synthesized metal complexes were then evaluated against two Gram-negative and two Gram-positive bacterial strains. Triphenyl tin complex emerged as potent antibacterial agent with MIC value of 8 μg/ml each against Shigellaspp, Salmonella typhi and Staphylococcus aureus. While, the MIC value against Streptococcus pneumoniae is 4 μg/ml. Computational docking studies were carried out on molecular targets to interpret the results of antioxidant and antibacterial activities. Based on the results, it may be inferred that the metal complexes of ursolic acid are more active as compared to the parent drug and may be proved for some other pharmacological potential by further analysis.

  7. Metal based new triazoles: Their synthesis, characterization and antibacterial/antifungal activities

    Science.gov (United States)

    Sumrra, Sajjad H.; Chohan, Zahid H.

    2012-12-01

    A series of new triazoles and their oxovanadium(IV) complexes have been synthesized, characterized and evaluated for antibacterial/antifungal properties. The new Schiff bases ligands (L1)-(L5) were prepared by the condensation reaction of 3,5-diamino-1,2,4-triazole with 2-hydroxy-1-naphthaldehyde, pyrrole-2-carboxaldehyde, pyridine-2-carboxaldehyde, 2-acetyl pyridine and 2-methoxy benzaldehyde. The structures of the ligands have been established on the basis of their physical, spectral (IR, 1H and 13C NMR and mass spectrometry) and elemental analytical data. The prepared ligands were used to synthesize their oxovanadium(IV) complexes (1)-(5) which were also characterized by their physical, spectral and analytical data and proposed to have a square pyramidal geometry. The ligands and their complexes were screened for in vitro antibacterial activity against six bacterial species such as, Escherichia coli, Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, and Bacillus subtilis and for in vitro antifungal activity against six fungal strains, Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani, and Candida glabrata. Cytotoxic nature of the compounds was also reported using brine shrimp bioassay method against Artemia salina.

  8. Metal complexes as antibacterial agents: Synthesis, characterization and antibacterial activity of some 3d metal complexes of sulphadimidine

    Directory of Open Access Journals (Sweden)

    Adedibu Clement Tella

    2010-06-01

    Full Text Available Metal complexes of Sulphadimidine(SAD were synthesized.The complexes were formulated as [Co(SAD2Cl2], [Cu(SAD2 (H2O2], [Ni (SAD2 Cl2 H2O], [Cd (SAD2 Br2], [Fe (SAD3](H­2O­3 and [Mn (SAD2Cl2] characterized by elemental Analysis, conductivity, IR , UV-Vis, Magnet moment and 1H-NMR and Mass spectroscopies. Co(II, Mn (II,  and Ni(II sulphadimidine complexes consist of metal ion which coordinates through amino nitrogen of the terminal NH2 group and oxygen of sulfonamidic group of the two molecules of sulphadimidine ligand and two halide ions to form octahedral structure while Cd(II coordinates with sulphadimidine through amino nitrogen of the terminal NH2 group with two bromine ions to complete tetrahedral structure. In Cu(II sulphadimidine complex, copper ion coordinates through both pyrimidinic nitrogen (heterocyclic nitrogen and sulfonamidic nitrogen of the two molecules of sulphadimidine. Fe(III coordinates to three molecules of sulphadimidine through heterocyclic nitrogen (pyrimidinic nitrogen and sulfonamidic nitrogen,with three molecules of water outside the coordination sphere. Both Fe(III and Cu(II complexes exhibit octahedral geometry. The antibacterial activity of the complexes and the ligands was investigated against Esherichia coli,  Staphylococcus aureus and Klebsiella pneumonia .  The data obtained revealed that the complexes showed greater activity against the three micro-organisms when compared to parent compound. Stability constant of the complexes were evaluated for the metal salts, the order of stability constant b was found to be Cu (II > Fe (III >Ni(II> Co (II > Cd (II.The values of stability constant (b was found to be log 6.31, 5.93, 5.29, 4.63 and 3.92, respectively. The stability constant data revealed that this ligand may be used as antidote or chelating agent for medical treatment of metals overload or poisoning.

  9. Synthesis, characterization and antibacterial activity of superparamagnetic nanoparticles modified with glycol chitosan

    Directory of Open Access Journals (Sweden)

    Baskaran Stephen Inbaraj, Tsung-Yu Tsai and Bing-Huei Chen

    2012-01-01

    Full Text Available Iron oxide nanoparticles (IONPs were synthesized by coprecipitation of iron salts in alkali media followed by coating with glycol chitosan (GC-coated IONPs. Both bare and GC-coated IONPs were subsequently characterized and evaluated for their antibacterial activity. Comparison of Fourier transform infrared spectra and thermogravimetric data of bare and GC-coated IONPs confirmed the presence of GC coating on IONPs. Magnetization curves showed that both bare and GC-coated IONPs are superparamagnetic and have saturation magnetizations of 70.3 and 59.8 emu g−1, respectively. The IONP size was measured as ~8–9 nm by transmission electron microscopy, and their crystal structure was assigned to magnetite from x-ray diffraction patterns. Both bare and GC-coated IONPs inhibited the growths of Escherichia coli ATCC 8739 and Salmonella enteritidis SE 01 bacteria better than the antibiotics linezolid and cefaclor, as evaluated by the agar dilution assay. GC-coated IONPs showed higher potency against E. coli O157:H7 and Staphylococcus aureus ATCC 10832 than bare IONPs. Given their biocompatibility and antibacterial properties, GC-coated IONPs are a potential nanomaterial for in vivo applications.

  10. Synthesis, characterization and antibacterial activity of superparamagnetic nanoparticles modified with glycol chitosan

    Science.gov (United States)

    Inbaraj, Baskaran Stephen; Tsai, Tsung-Yu; Chen, Bing-Huei

    2012-02-01

    Iron oxide nanoparticles (IONPs) were synthesized by coprecipitation of iron salts in alkali media followed by coating with glycol chitosan (GC-coated IONPs). Both bare and GC-coated IONPs were subsequently characterized and evaluated for their antibacterial activity. Comparison of Fourier transform infrared spectra and thermogravimetric data of bare and GC-coated IONPs confirmed the presence of GC coating on IONPs. Magnetization curves showed that both bare and GC-coated IONPs are superparamagnetic and have saturation magnetizations of 70.3 and 59.8 emu g-1, respectively. The IONP size was measured as ~8-9 nm by transmission electron microscopy, and their crystal structure was assigned to magnetite from x-ray diffraction patterns. Both bare and GC-coated IONPs inhibited the growths of Escherichia coli ATCC 8739 and Salmonella enteritidis SE 01 bacteria better than the antibiotics linezolid and cefaclor, as evaluated by the agar dilution assay. GC-coated IONPs showed higher potency against E. coli O157:H7 and Staphylococcus aureus ATCC 10832 than bare IONPs. Given their biocompatibility and antibacterial properties, GC-coated IONPs are a potential nanomaterial for in vivo applications.

  11. Synthesis, characterization and antibacterial activity of superparamagnetic nanoparticles modified with glycol chitosan

    International Nuclear Information System (INIS)

    Stephen Inbaraj, Baskaran; Tsai, Tsung-Yu; Chen, Bing-Huei

    2012-01-01

    Iron oxide nanoparticles (IONPs) were synthesized by coprecipitation of iron salts in alkali media followed by coating with glycol chitosan (GC-coated IONPs). Both bare and GC-coated IONPs were subsequently characterized and evaluated for their antibacterial activity. Comparison of Fourier transform infrared spectra and thermogravimetric data of bare and GC-coated IONPs confirmed the presence of GC coating on IONPs. Magnetization curves showed that both bare and GC-coated IONPs are superparamagnetic and have saturation magnetizations of 70.3 and 59.8 emu g −1 , respectively. The IONP size was measured as ∼8–9 nm by transmission electron microscopy, and their crystal structure was assigned to magnetite from x-ray diffraction patterns. Both bare and GC-coated IONPs inhibited the growths of Escherichia coli ATCC 8739 and Salmonella enteritidis SE 01 bacteria better than the antibiotics linezolid and cefaclor, as evaluated by the agar dilution assay. GC-coated IONPs showed higher potency against E. coli O157:H7 and Staphylococcus aureus ATCC 10832 than bare IONPs. Given their biocompatibility and antibacterial properties, GC-coated IONPs are a potential nanomaterial for in vivo applications.

  12. Synthesis and Characterization of Fe3O4 Nanoparticles Modified with Polyethylene Glycol as Antibacterial Material

    Directory of Open Access Journals (Sweden)

    Poedji Loekitowati Hariani

    2018-05-01

    Full Text Available The iron oxide (Fe3O4 nanoparticles modified with polyethylene glycol (PEG was synthesized by co-precipitation methods using ferric and ferrous ions as the precursors. Further, the antibacterial activity was performed against gram-positive and gram-negative bacteria. The Fe3O4-PEG was characterized using X-Ray Diffraction (XRD, Fourier Transform Infra Red (FTIR, Scanning Electron Microscopy (SEM with energy dispersive X-Ray analysis (EDAX and Vibrating Sample Magnetometer (VSM. The particle size of Fe3O4-PEG calculated using XRD is 46.2 nm. The study confirmed that Fe3O4-PEG is superparamagnetic and has a saturation magnetization of 56.43 emu/g. The prepared Fe3O4-PEG gives the effect of both gram-positive and gram-negative pathogenic bacterial strains hence this material has potential utilization in the field of pharmaceutical and biomedical in the future.

  13. Novel conductive polypyrrole/zinc oxide/chitosan bionanocomposite: synthesis, characterization, antioxidant, and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Ebrahimiasl S

    2014-12-01

    Full Text Available Saeideh Ebrahimiasl,1,2 Azmi Zakaria,3 Anuar Kassim,4 Sri Norleha Basri4 1Department of Nanotechnology, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Malaysia; 2Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran; 3Department of Physics, Universiti Putra Malaysia, Serdang, Malaysia; 4Department of Chemistry, Universiti Putra Malaysia, Serdang, Malaysia Abstract: An antibacterial and conductive bionanocomposite (BNC film consisting of polypyrrole (Ppy, zinc oxide (ZnO nanoparticles (NPs, and chitosan (CS was electrochemically synthesized on indium tin oxide (ITO glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO–Ppy/CS and ITO–Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%, while it was increased gradually for higher ratios (10, 15, and 20 wt%. The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa. The thermal stability and physical properties of BNC films were

  14. A silver complex with tryptophan: Synthesis, structural characterization, DFT studies and antibacterial and antitumor assays in vitro

    Science.gov (United States)

    Carvalho, Marcos A.; de Paiva, Raphael E. F.; Bergamini, Fernando R. G.; Gomes, Alexandre F.; Gozzo, Fábio C.; Lustri, Wilton R.; Formiga, André L. B.; Shishido, Silvia M.; Ferreira, Carmen V.; Corbi, Pedro P.

    2013-01-01

    The synthesis, spectroscopic characterization and biological assays of a new silver(I) complex with L-tryptophan (TRP) are presented. Elemental and thermal analyses and ESI-QTOF mass spectrometric measurements of the solid compound suggest the composition AgC11H11N2O2. Infrared and solid-state NMR analyses indicate coordination of TRP to Ag(I) ion through the nitrogen of the NH2 group and also through the oxygen of carboxylate group. Theoretical (DFT) calculations permit proposing an optimized geometry for the complex. Antibacterial assays indicated that the Ag-TRP complex is effective against Staphylococcus aureus and Enterococcus faecalis (Gram-positive), and Pseudomonas aeruginosa and Escherichia coli (Gram-negative) bacterial strains. The complex was also cytotoxic against Panc-1 (human pancreatic carcinoma) and SK-Mel 103 (human melanoma) cells.

  15. Synthesis and characterization of crosslinked polyisothiouronium methylstyrene nanoparticles of narrow size distribution for antibacterial and antibiofilm applications.

    Science.gov (United States)

    Cohen, Sarit; Gelber, Chen; Natan, Michal; Banin, Ehud; Corem-Salkmon, Enav; Margel, Shlomo

    2016-07-07

    Isothiouronium salts are well known in their variety of antimicrobials activities. The use of polymeric biocides, polymers with antimicrobial activities, is expected to enhance the efficacy of some existing antimicrobial agents, thus minimizing the environmental problems accompanying conventional antimicrobials. The current manuscript describes the synthesis and characterization of crosslinked polyisothiouronium methylstyrene (PITMS) nanoparticles (NPs) of narrow size distribution by dispersion co-polymerization of the monomer isothiouronium methylstyrene with the crosslinking monomer ethylene glycol dimetacrylate. The effect of total monomer, crosslinker and initiator concentrations on the size and size distribution of the formed NPs was also elucidated. The bactericidal activity of PITMS NPs of 67 ± 8 nm diameter was illustrated for 4 bacterial pathogens: Listeria innocua, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In order to demonstrate the potential of these PITMS NPs as inhibitor of biofilm formation, polyethylene terephthalate (PET) films were thin-coated with the PITMS NPs. The formed PET/PITMS films reduced the viability of the biofilm of Listeria by 2 orders of magnitude, making the coatings excellent candidates for further development of non-fouling surfaces. In addition, PITMS NP coatings were found to be non-toxic in HaCaT cells. The high antibacterial activity and effective inhibition of bacterial adsorption indicate the potential of these nanoparticles for development of new types of antibacterial and antibiofilm additives.

  16. Green Synthesis, Characterization, and Antibacterial Activity of Silver/Polystyrene Nanocomposite

    Directory of Open Access Journals (Sweden)

    Manal A. Awad

    2015-01-01

    Full Text Available A novel, nontoxic, simple, cost-effective and ecofriendly technique was used to synthesize green silver nanoparticles (AgNPs. The AgNPs were synthesized using orange peel extract as a reducing agent for silver nitrate salt (AgNO3. The particle size distribution of AgNPs was determined by Dynamic Light Scattering (DLS. The average size of silver nanoparticles was 98.43 nm. The stable dispersion of silver nanoparticles was added slowly to polystyrene solution in toluene maintaining the temperature at 70°C. The AgNPs/polystyrene (PS nanocomposite solution was cast in a petri dish. The silver nanoparticles encapsulated within polymer chains were characterized by X-ray diffraction (XRD and Scanning Electron Microscopy (SEM equipped with Energy Dispersive Spectroscopy (EDS in addition to Transmission Electron Microscopy (TEM. The green AgNPs/PS nanocomposite film exhibited antimicrobial activity against Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae and Salmonella, and Gram-positive bacteria Staphylococcus aureus. Thus, the key findings of the work include the use of a safe and simple AgNPs/PS nanocomposite which had a marked antibacterial activity which has a potential application in food packaging.

  17. Cellulose aerogels functionalized with polypyrrole and silver nanoparticles: In-situ synthesis, characterization and antibacterial activity.

    Science.gov (United States)

    Wan, Caichao; Li, Jian

    2016-08-01

    Green porous and lightweight cellulose aerogels have been considered as promising candidates to substitute some petrochemical host materials to support various nanomaterials. In this work, waste wheat straw was collected as feedstock to fabricate cellulose hydrogels, and a green inexpensive NaOH/polyethylene glycol solution was used as cellulose solvent. Prior to freeze-drying treatment, the cellulose hydrogels were integrated with polypyrrole and silver nanoparticles by easily-operated in-situ oxidative polymerization of pyrrole using silver ions as oxidizing agent. The tri-component hybrid aerogels were characterized by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, selected area electron diffraction, X-ray photoelectron spectroscopy, and X-ray diffraction. Moreover, the antibacterial activity of the hybrid aerogels against Escherichia coli (Gram-negative), Staphylococcus aureus (Gram-positive) and Listeria monocytogenes (intracellular bacteria) was qualitatively and quantitatively investigated by parallel streak method and determination of minimal inhibitory concentration, respectively. This work provides an example of combining cellulose aerogels with nanomaterials, and helps to develop novel forms of cellulose-based functional materials. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Highly crystalline zinc incorporated hydroxyapatite nanorods' synthesis, characterization, thermal, biocompatibility, and antibacterial study

    Science.gov (United States)

    Udhayakumar, Gayathri; Muthukumarasamy, N.; Velauthapillai, Dhayalan; Santhosh, Shanthi Bhupathi

    2017-10-01

    Highly crystalline zinc incorporated hydroxyapatite (Zn-HAp) nanorods have been synthesized using microwave irradiation method. To improve bioactivity and crystallinity of pure HAp, zinc was incorporated into it. As-synthesized samples were characterized by Fourier transform-infrared spectroscopy (FT-IR), X-ray diffraction, field-emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDAX), high-resolution transmission electron microscopy (HRTEM), and the thermal and crystallinity behavior of Zn-HAp nanoparticle were studied by thermogravimetry (TGA) and differential scanning calorimetry (DSC). Antibacterial activity of the as-synthesized nanorods was evaluated against two prokaryotic strains ( Escherichia coli and Staphylococcus aureus). The FT-IR studies show the presence of hydroxide and phosphate functional groups. HRTEM and FESEM images showed highly crystalline rod-shaped nanoparticles with the diameter of about 50-60 nm. EDAX revealed the presence of Ca, Zn, P, and O in the prepared samples. The crystallinity and thermal stability were further confirmed by TGA-DSC analysis. The biocompatibility evaluation results promoted that the Zn-HAp nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopaedic application.

  19. Synthesis and characterization of waterborne polyurethane/Cu(II)-loaded hydroxyapatite nanocomposites with antibacterial activity.

    Science.gov (United States)

    Zhao, Cai-Xia; Zhang, Wei-De; Mai, Ai-Ping; Huang, Xiao-Mo; Ouyang, You-Sheng

    2011-08-01

    A novel kind of environmentally friendly nanocomposites, waterborne polyurethane (WBPU)/Cu(II)-loaded hydroxyapatite (CuHAp), with improved physical properties and antibacterial activity have been prepared via in-situ polymerization from functionalized CuHAp nanoparticles (CuHAp NPs). The interaction of the CuHAp NPs with isophorone diisocyanate to form the functionalized CuHAp NPs containing isocyanate groups (CuHAp-g-NCO) has been studied. The microstructure and particle distribution of the nanocomposites were observed using scanning electron microscopy. The improvements of mechanical properties, thermal stability and water resistance of the nanocomposites have also been evaluated. Finally, the antibacterial activity was tested against G(-) Escherichia coli and G(+) Staphylococcus aureus by the zone of inhibition test and the direct contact test. The long-lasting antibacterial activity was studied by measuring antibacterial ability of the nanocomposites after being immersed in water. The results indicate that WBPU incorporation with CuHAp NPs shows strong antibacterial activity upon contact, and long-lasting antibacterial property.

  20. Alpha amylase assisted synthesis of TiO{sub 2} nanoparticles: Structural characterization and application as antibacterial agents

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, Razi; Mohsin, Mohd [Department of Biosciences, Jamia Millia Islamia, New Delhi 110025 (India); Ahmad, Tokeer [Department of Chemistry, Jamia Millia Islamia, New Delhi 110025 (India); Sardar, Meryam, E-mail: msardar@jmi.ac.in [Department of Biosciences, Jamia Millia Islamia, New Delhi 110025 (India)

    2015-02-11

    Graphical abstract: - Highlights: • Green synthesis of TiO{sub 2} nanoparticles using an enzyme alpha amylase has been described. • The morphology and shape depends upon the concentration of the alpha amylase enzyme. • The biosynthesized nanoparticles show good bactericidal effect against both gram positive and gram negative bacteria. • The bactericidal effect was further confirmed by Confocal microscopy and TEM. - Abstract: The enzyme alpha amylase was used as the sole reducing and capping agent for the synthesis of TiO{sub 2} nanoparticles. The biosynthesized nanoparticles were characterized by X-ray diffraction (XRD) and transmission electron microscopic (TEM) methods. The XRD data confirms the monophasic crystalline nature of the nanoparticles formed. TEM data shows that the morphology of nanoparticles depends upon the enzyme concentration used at the time of synthesis. The presence of alpha amylase on TiO{sub 2} nanoparticles was confirmed by FTIR. The nanoparticles were investigated for their antibacterial effect on Staphylococcus aureus and Escherichia coli. The minimum inhibitory concentration value of the TiO{sub 2} nanoparticles was found to be 62.50 μg/ml for both the bacterial strains. The inhibition was further confirmed using disc diffusion assay. It is evident from the zone of inhibition that TiO{sub 2} nanoparticles possess potent bactericidal activity. Further, growth curve study shows effect of inhibitory concentration of TiO{sub 2} nanoparticles against S. aureus and E. coli. Confocal microscopy and TEM investigation confirm that nanoparticles were disrupting the bacterial cell wall.

  1. Synthesis, characterization, crystal structures, QSAR study and antibacterial activities of organotin bisphosphoramidates

    Czech Academy of Sciences Publication Activity Database

    Gholivand, K.; Valmoozi, A.A.E.; Gholami, A.; Dušek, Michal; Eigner, Václav; Abolghasemi, S.

    2016-01-01

    Roč. 806, Mar (2016), s. 33-44 ISSN 0022-328X R&D Projects: GA ČR GA15-12653S Institutional support: RVO:68378271 Keywords : bisphosphoramidate * organotin compounds * crystal structure * antibacterial activity * QSAR Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.184, year: 2016

  2. Novel copper (Cu) loaded core-shell silica nanoparticles with improved Cu bioavailability: synthesis, characterization and study of antibacterial properties.

    Science.gov (United States)

    Maniprasad, Pavithra; Santra, Swadeshmukul

    2012-08-01

    We report synthesis of a novel core-shell silica based antimicrobial nanoparticles where the silica shell has been engineered to accommodate copper (Cu). Synthesis of the core-shell Cu-silica nanoparticle (C-S CuSiO2NP) involves preparation of base-hydrolyzed Stöber silica "seed" particles first, followed by the acid-catalyzed seeded growth of the Cu-silica shell layer around the core. The Scanning Electron Microscopy (SEM) and the Transmission Electron Microscopy (TEM) measured the seed particle size to be -380 nm and the shell thickness to be -35 nm. The SEM particle characterization confirms formation of highly monodispersed particles with smooth surface morphology. Characterization of particle size distribution in solution by Dynamic Light Scattering (DLS) technique was fairly consistent with the electron microscopy results. Loading of Cu to nanoparticles was confirmed by the SEM-Energy Dispersive X-Ray Spectroscopy (EDS) and Atomic Absorption Spectroscopy (AAS). The Cu loading was estimated to be 0.098 microg of metallic copper per mg of C-S CuSiO2NP material by the AAS technique. Antibacterial efficacy of C-S CuSiO2NP was evaluated against E. coli and B. subtilis using Cu hydroxide ("Insoluble" Cu compound, sub-micron size particles) as positive control and silica "seed" particles (without Cu loading) as negative control. Bacterial growth in solution was measured against different concentrations of C-S CuSiO2NP to determine the Minimum Inhibitory Concentration (MIC) value. The estimated MIC values were 2.4 microg metallic Cu/mL for both E. coli and B. subtilis. Bac-light fluorescence microscopy based assay was used to count relative population of the live and dead bacteria cells. Antibacterial study clearly shows that C-S CuSiO2NP is more effective than insoluble Cu hydroxide particles at equivalent metallic Cu concentration, suggesting improvement of Cu bioavailability (i.e., more soluble Cu) in C-SCuSiO2NP material due to its core-shell design.

  3. New bioactive silver(I) complexes: Synthesis, characterization, anticancer, antibacterial and anticarbonic anhydrase II activities

    Science.gov (United States)

    Ozdemir, Ummuhan O.; Ozbek, Neslihan; Genc, Zuhal Karagoz; İlbiz, Firdevs; Gündüzalp, Ayla Balaban

    2017-06-01

    Silver(I) complexes of alkyl sulfonic acide hydrazides were newly synthesized as homologous series. Methanesulfonic acide hydrazide (L1), ethanesulfonic acide hydrazide (L2), propanesulfonic acide hydrazide (L3) and butanesulfonic acide hydrazide (L4) were used for complexation with Ag(I) ions. The silver complexes obtained in the mol ratio of 1:2 have the structural formula as Ag(L1)2NO3 (I), Ag(L2)2NO3 (II), Ag(L3)2NO3(III), (Ag(L4)2NO3 (IV). The Ag(I) complexes exhibit distorted linear two-fold coordination in [AgL2]+ cations with uncoordinated nitrates. Ligands are chelated with silver(I) ions through unsubstituted primary nitrogen in hydrazide group. Ag(I) complexes were characterized by using elemental analysis, spectroscopic methods (FT-IR, LC-MS), magnetic susceptibility and conductivity measurements. Silver(I) complexes were optimized using PBEPBE/LanL2DZ/DEF2SV basic set performed by DFT method with the Gaussian 09 program package. The geometrical parameters, frontier molecular orbitals (HOMOs and LUMOs) and molecular electrostatic potential (MEP) mapped surfaces of the optimized geometries were also determined by this quantum set. The anticancer activities of silver(I) complexes on MCF-7 human breast cancer cell line were investigated by comparing IC50 values. The antibacterial activities of complexes were studied against Gram positive bacteria; S. aureus ATCC 6538, B. subtilis ATCC 6633, B. cereus NRRL-B-3711, E. faecalis ATCC 29212 and Gram negative bacteria; E. coli ATCC 11230, P. aeruginosa ATCC 15442, K. pneumonia ATCC 70063 by using disc diffusion method. The inhibition activities of Ag(I) complexes on carbonic anhydrase II enzyme (hCA II) were also investigated by comparing IC50 and Ki values. The biological activity screening shows that Ag(I) complex of butanesulfonicacidehydrazide (IV) has the highest activity against tested breast cancer cell lines MCF-7, Gram positive/Gram negative bacteria and carbonic anhydrase II (hCA II) isoenzyme.

  4. In situ green synthesis and characterization of sericin-silver nanoparticle composite with effective antibacterial activity and good biocompatibility.

    Science.gov (United States)

    He, Huawei; Tao, Gang; Wang, Yejing; Cai, Rui; Guo, Pengchao; Chen, Liqun; Zuo, Hua; Zhao, Ping; Xia, Qingyou

    2017-11-01

    Silver nanoparticle has been widely applied to a variety of fields for its outstanding antimicrobial activity. However, the stability of silver nanoparticle limits its application under certain conditions. Thus, improving the stability of silver nanoparticle via biosynthesis is a promising shortcut to expand its application. Sericin from silkworm cocoon has good hydrophilicity, reaction activity, biocompatibility and biodegradability. In this study, we developed a novel, simple, one-step biosynthesis method to prepare sericin-silver nanoparticle composite in situ in solution. Sericin served as the reductant of silver ion, the dispersant and stabilizer of the prepared sericin-silver nanoparticle composite. Natural light was the only power source used to catalyze the synthesis of silver nanoparticle in situ in solution. The novel sericin-silver nanoparticle composite was characterized by ultraviolet-visible and fluorescence spectroscopy, X-ray diffraction, transmission electron microscopy and fourier transform infrared spectroscopy. The results showed silver nanoparticle could be synthesized through the reduction of AgNO 3 by the phenolic hydroxyl group of tyrosine residues of sericin under the catalysis of natural light. The synthesized silver nanoparticle had good crystalline, size distribution and long-term stability at room temperature. Light irradiation was essential for the preparation of sericin-silver nanoparticle composite. The antibacterial activity assay showed 25mg/L and 100mg/L were the minimum concentrations of sericin-silver nanoparticle composite required to inhibit the growth of Staphylococcus aureus and kill this bacterium, respectively. The cytotoxicity assay showed cell viability and cell growth were almost not affected by sericin-silver nanoparticle composite under the concentration of 25mg/L. Our study suggested the preparation of sericin-silver nanoparticle composite was environmentally friendly and energy conservation, and the prepared sericin

  5. Synthesis, structural characterization of nano ZnTiO3 ceramic: An effective azo dye adsorbent and antibacterial agent

    Directory of Open Access Journals (Sweden)

    R.S. Raveendra

    2014-12-01

    Full Text Available Nanocrystalline meta-zinc titanate (ZnTiO3 ceramic was prepared using a self-propagating solution combustion synthesis (SCS for the first time using urea as fuel. The product was calcined at 800 °C for 2 h to improve the crystallinity. Powder X-ray diffraction (PXRD, Fourier transform infrared spectroscopy (FTIR, scanning electron microscopy (SEM, energy-dispersive X-ray spectroscopy (EDAX, high resolution transmission electron microscopy (HR-TEM and UV–vis absorption spectroscopy were used to characterize the final product. PXRD results show that the ilmenite type rhombohedral structure was formed when the sample was calcined at 800 °C for 2 h. Adsorption experiments were performed with cationic malachite green (MG dye. ∼96% dye was adsorbed onto nanocrystalline ZnTiO3 ceramic at pH 9 for 30 min of the contact time. The optimum adsorbent dose was found to be 0.45 g/L of dye. Langmuir–Hinshelwood model was used to study adsorption kinetics and first order kinetic model best describes the MG adsorption on ZnTiO3. Antibacterial activity was investigated against gram negative Klebsiella aerogenes, Pseudomonas desmolyticum, Escherichia coli, and gram positive Staphylococcus aureus bacteria by agar well diffusion method. Nanocrystalline ZnTiO3 ceramic showed significant effect on all the four bacterial strains at the concentration of 1000 and 1500 μg per well.

  6. Synthesis, Characterization, and Evaluation of the Antibacterial Activity of Allophylus serratus Leaf and Leaf Derived Callus Extracts Mediated Silver Nanoparticles

    Directory of Open Access Journals (Sweden)

    Kero Jemal

    2017-01-01

    Full Text Available Allophylus serratus mediated silver nanoparticles biosynthesis, characterization, and antimicrobial activity were described. The synthesis of silver nanoparticles was confirmed by visual observation: UV-Vis spectrum, X-ray diffraction (XRD, Scanning Electron Microscopy (SEM, Energy Dispersive Spectroscopy (EDS, and Fourier Transform Infra-Red (FTIR. UV-Vis spectroscopy studies showed that the absorption spectra of synthesized silver nanoparticles from leaf and callus extracts had absorbance peak range of 440 nm and 445 nm, respectively. The X-RD pattern revealed the presence of crystalline, dominantly spherical silver nanoparticles in the sample having size ranging from 42 to 50 nm. The XRD peaks 38.2°, 44.1°, 64.1°, and 77.0° for leaf extract and 38.1°, 44.3°, 64.5°, 77.5°, and 81.33° for callus extract can be assigned the plane of silver crystals (111, (200, (220, and (311, respectively, and indicate that the silver nanoparticles are face-centered, cubic, and crystalline in nature. SEM and EDS analysis also confirmed the presence of silver nanoparticles. The FTIR results showed the presence of some biomolecules in extracts that act as reducing and capping agent for silver nanoparticles biosynthesis. The synthesized silver nanoparticles showed significant antibacterial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa.

  7. Synthesis and characterization of chitosan and grape polyphenols stabilized palladium nanoparticles and their antibacterial activity.

    Science.gov (United States)

    Amarnath, Kanchana; Kumar, Jayanthi; Reddy, Tejesh; Mahesh, Vakka; Ayyappan, Senniyanallur Rathakrishnan; Nellore, Jayshree

    2012-04-01

    Based on enhanced effectiveness, the new age drugs are nanoparticles of polymers, metals or ceramics, which can combat conditions like cancer and fight human pathogens like bacteria. In this present study we aimed for a green approach to synthesize palladium nanoparticles by reducing palladium chloride salts with nontoxic and biodegradable polymeric chitosan and grape polyphenols and confirmed by FTIR, TEM, SEM and UV-spectroscopy. We also extended our study to show the efficacy of the grape and chitosan impregnated palladium nanoparticles as an antibacterial agent against Escherichia coli. Antibacterial assays were carried out with a representative gram-negative bacterium, E. coli and a gram-positive bacterium, Staphylococcus aureus. Commendable efforts have been made to explore this property using electron microscopy, which has revealed size dependent interaction of palladium nanoparticles conjugates with bacteria by disrupting cell membranes and the leakage of cytoplasm. Therefore, the observed results imply that grape and chitosan-based nano palladium conjugates prepared in our present system are promising candidates for a wide range of biomedical and general applications. Copyright © 2011 Elsevier B.V. All rights reserved.

  8. Antibacterial continuous nanofibrous hybrid yarn through in situ synthesis of silver nanoparticles: Preparation and characterization

    Energy Technology Data Exchange (ETDEWEB)

    Barani, Hossein, E-mail: barani@birjand.ac.ir

    2014-10-01

    Nanofibrous hybrid yarns of polyvinyl alcohol (PVA) and poly-L-lactide acid (PLLA) with the antibacterial activity were prepared that contains 0, 5, 10, 20, and 30 wt.% of silver nanoparticles according to the PVA polymer content. This was performed by electrospinning using distilled water and 2, 2, 2-trifluoroethanol as a solvent for PVA and PLLA respectively, and sodium borohydride was used as a reducing agent. The scanning electron microscope observation confirmed the formation of AgNPs into the PVA nanofiber structure, and they were uniform, bead free, cylindrical and smooth. The diameter of hybrid yarns and their nanofiber component was decreased as the silver nitrate concentration in electrospinning solutions was increased. The differential scanning calorimetry results indicated that the silver nanoparticles can form interactions with polymer chains and decrease the melting enthalpy. The mechanical analysis showed a lower stress and strain at break of the AgNP-loaded nanofibrous hybrid yarns than the unloaded hybrid yarn. However, there wasn't a statistically significant difference between the strain at break of electrospun nanofibrous hybrid yarns. Moreover, the bactericidal efficiency of all loaded samples was over 99.99%. - Highlights: • Nanofibrous hybrid yarns of PVA/PLLA with antibacterial activity were prepared. • The diameter of nanofibers was decreased as the AgNP concentration was increased. • AgNPs make interactions with amorphous phase of polymer and increase the Tg. • All loaded samples presented a good bactericidal and bacteriostatic efficiency.

  9. A novel and green biomaterial based silver nanocomposite hydrogel: synthesis, characterization and antibacterial effect.

    Science.gov (United States)

    Bardajee, Ghasem R; Hooshyar, Zari; Rezanezhad, Habib

    2012-12-01

    In the present study, we report a facile and eco-friendly method for the preparation of a novel silver nanocomposite hydrogel (SNH) based on poly(acrylic acid) (PAA) grafted onto salep as a water soluble polysaccharide backbone. The presence of inorganic silver nanoparticles (nano-Ag) in the hydrogel was confirmed by thermo-gravimetric (TG) analysis. The TEM images illustrated the presence of embedded nano-Ag throughout the hydrogel matrix. In addition, the transmission electron microscopy (TEM) images showed that the formed nano-Ag had an average particle size of 5-10 nm. The potential of obtained SNH was examined for Tetracycline hydrochloride (TH) release in simulated colon conditions. Lastly, the in vitro antibacterial properties of the obtained optimum sample were successfully evaluated against gram-negative and gram-positive bacteria. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. SYNTHESIS AND CHARACTERIZATION OF SODIUM SOAP FROM NYAMPLUNG SEED OIL (Calophyllum inophyllum L. AND TEST ANTIBACTERIAL ACTIVITY AGAINST Staphilococus aureus

    Directory of Open Access Journals (Sweden)

    Mochamad Chasani

    2015-05-01

    Full Text Available This research was used nyamplung seed oil as antibacterial soap material. The soap from nyamplung seed oil was performed by saponification reactions, characterization of soap based on SNI 06-3632-1994. The antibacterial activity was tested against bacteria Staphylococus aureus. The result of this research showed that soap had yellow color with weight 13,028 g from 10,028 g nyamplung seed oil. The characteristic of soap based on SNI were water content of 25,287%, fatty acid of 72,177%, free alkali 0,082%, unsoap fatty or free fatty 0,834%, and mineral oil was negative. Soap base on nyamplung seed oil have antibacterial activity against bacteria Staphylococus aureus with diameter of inhibition zone 14,701 mm.

  11. Characterization of Silver Nanoparticle In Situ Synthesis on Porous Sericin Gel for Antibacterial Application

    OpenAIRE

    Tao, Gang; Liu, Lina; Wang, Yejing; Chang, Huaipu; Zhao, Ping; Zuo, Hua; He, Huawei

    2016-01-01

    Sericin from Bombyx mori cocoon has good hydrophilicity, reaction activity, biocompatibility, and biodegradability, which has shown great potentials for biomedical materials. Here, an ultraviolet light-assisted in situ synthesis approach is developed to immobilize silver nanoparticles on the surface of sericin gel. The amount of silver nanoparticles immobilized on the surface of sericin gel could be regulated by the irradiation time. The porous structure and property of sericin gel were not a...

  12. Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics.

    Science.gov (United States)

    Singh, Richa; Wagh, Priyanka; Wadhwani, Sweety; Gaidhani, Sharvari; Kumbhar, Avinash; Bellare, Jayesh; Chopade, Balu Ananda

    2013-01-01

    The development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. The present study aims to biosynthesize silver nanoparticles (AgNPs) using a cell-free extract of Acinetobacter spp. and evaluate their antibacterial activity. Eighteen strains of Acinetobacter were screened for AgNP synthesis. AgNPs were characterized using various techniques. Reaction parameters were optimized, and their effect on the morphology of AgNPs was studied. The synergistic potential of AgNPs on 14 antibiotics against seven pathogens was determined by disc-diffusion, broth-microdilution, and minimum bactericidal concentration assays. The efficacy of AgNPs was evaluated as per the minimum inhibitory concentration (MIC) breakpoints of the Clinical and Laboratory Standards Institute (CLSI) guidelines. Only A. calcoaceticus LRVP54 produced AgNPs within 24 hours. Monodisperse spherical nanoparticles of 8-12 nm were obtained with 0.7 mM silver nitrate at 70°C. During optimization, a blue-shift in ultraviolet-visible spectra was seen. X-ray diffraction data and lattice fringes (d =0.23 nm) observed under high-resolution transmission electron microscope confirmed the crystallinity of AgNPs. These AgNPs were found to be more effective against Gram-negative compared with Gram-positive microorganisms. Overall, AgNPs showed the highest synergy with vancomycin in the disc-diffusion assay. For Enterobacter aerogenes, a 3.8-fold increase in inhibition zone area was observed after the addition of AgNPs with vancomycin. Reduction in MIC and minimum bactericidal concentration was observed on exposure of AgNPs with antibiotics. Interestingly, multidrug-resistant A. baumannii was highly sensitized in the presence of AgNPs and became susceptible to antibiotics except cephalosporins. Similarly, the vancomycin-resistant strain of Streptococcus mutans was also found to be susceptible to antibiotic treatment when AgNPs were added. These

  13. Characterization of Silver Nanoparticle In Situ Synthesis on Porous Sericin Gel for Antibacterial Application

    Directory of Open Access Journals (Sweden)

    Gang Tao

    2016-01-01

    Full Text Available Sericin from Bombyx mori cocoon has good hydrophilicity, reaction activity, biocompatibility, and biodegradability, which has shown great potentials for biomedical materials. Here, an ultraviolet light-assisted in situ synthesis approach is developed to immobilize silver nanoparticles on the surface of sericin gel. The amount of silver nanoparticles immobilized on the surface of sericin gel could be regulated by the irradiation time. The porous structure and property of sericin gel were not affected by the modification of AgNPs, as evidenced by the observation of scanning electron microscopy, X-ray diffractometry, and Fourier transform infrared spectroscopy. Differential scanning calorimetry analysis showed that the modification of AgNPs increased the thermal stability of sericin gel. The growth curve of bacteria and inhibition zone assays suggested that the sericin gel modified with AgNPs had good antimicrobial activities against both Gram-negative and Gram-positive bacteria. This novel sericin has shown a great potential for biomedical purpose.

  14. Synthesis and characterization of novel benzohydrazide as potential antibacterial agents from natural product vanillin and wintergreen oil

    Science.gov (United States)

    Setyawati, Amri; Wahyuningsih, Tutik Dwi; Purwono, Bambang

    2017-03-01

    A chalcone-like benzohydrazide derivatives (3) has been synthesis from natural resources vanillin (1a) and wintergreen oil (1b). This compound was synthesis as modified natural resource antibacterial agent. Some modification was done to increase the biological activity. Bromide was introduced to the vanillin structure to increase the activity (2a), whereas Hydrazine monohydrate was reacted with wintergreen oil to make new nucleophile (2b). Furthermore, chalcone like benzohydrazide compound was synthesized by stirring 5-bromovanillin (2a) with salicyl hydrazine (2b) at room temperature for 2-3 hours. The product was analyzed by FTIR, GCMS, 1H- and 13C-NMR to confirm its structure. The result showed that 5-bromovanillin, salicyl hydrazine, and benzohydrazide were successfully synthesized with 98, 78, and 33% of yield respectively. The target molecule 3 achieved with yellowish color with m.p. 106-111 °C, 97% purity.

  15. Tridentate hydrazone metal complexes derived from cephalexin and 2-hydrazinopyridine: Synthesis, characterization and antibacterial activity

    Science.gov (United States)

    Anacona, J. R.; Rincones, Maria

    2015-04-01

    Metal(II) coordination compounds of a tridentate hydrazone ligand (HL) derived from the condensation of cephalexin antibiotic with 2-hydrazinopyridine were synthesized. The hydrazone ligand and mononuclear [ML(OAc)(H2O)] (M(II) = Mn, Co, Ni, Cu, Zn, Ag) complexes were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and 1H NMR spectral studies. The cephalexin 2-pyridinylhydrazone ligand HL behaves as a monoanionic tridentate NNO chelating agent. The biological applications of complexes have been studied on three bacteria strains (Escherichia coli, Acinetobacter baumannii and Enterococcus faecalis) by agar diffusion disc method.

  16. Synthesis, characterization, antibacterial activity, SOD mimic and interaction with DNA of drug based copper(II) complexes

    Science.gov (United States)

    Patel, Mohan N.; Dosi, Promise A.; Bhatt, Bhupesh S.; Thakkar, Vasudev R.

    2011-02-01

    Novel metal complexes of the second-generation quinolone antibacterial agent enrofloxacin with copper(II) and neutral bidentate ligands have been prepared and characterized with elemental analysis reflectance, IR and mass spectroscopy. Complexes have been screened for their in-vitro antibacterial activity against two Gram (+ve)Staphylococcus aureus, Bacillus subtilis, and three Gram (-ve)Serratia marcescens, Escherichia coli and Pseudomonas aeruginosa organisms using the double dilution technique. The binding of this complex with CT-DNA has been investigated by absorption titration, salt effect and viscosity measurements. Binding constant is ranging from 1.3 × 10 4-3.7 × 10 4. The cleavage ability of complexes has been assessed by gel electrophoresis using pUC19 DNA. The catalytic activity of the copper(II) complexes towards the superoxide anion (O 2rad -) dismutation was assayed by their ability to inhibit the reduction of nitroblue tetrazolium (NBT).

  17. Synthesis, characterization and antibacterial activity of biodegradable starch/PVA composite films reinforced with cellulosic fibre.

    Science.gov (United States)

    Priya, Bhanu; Gupta, Vinod Kumar; Pathania, Deepak; Singha, Amar Singh

    2014-08-30

    Cellulosic fibres reinforced composite blend films of starch/poly(vinyl alcohol) (PVA) were prepared by using citric acid as plasticizer and glutaraldehyde as the cross-linker. The mechanical properties of cellulosic fibres reinforced composite blend were compared with starch/PVA crossed linked blend films. The increase in the tensile strength, elongation percentage, degree of swelling and biodegradability of blend films was evaluated as compared to starch/PVA crosslinked blend films. The value of different evaluated parameters such as citric acid, glutaraldehyde and reinforced fibre to starch/PVA (5:5) was found to be 25 wt.%, 0.100 wt.% and 20 wt.%, respectively. The blend films were characterized using Fourier transform-infrared spectrophotometry (FTIR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA/DTA/DTG). Scanning electron microscopy illustrated a good adhesion between starch/PVA blend and fibres. The blend films were also explored for antimicrobial activities against pathogenic bacteria like Staphylococcus aureus and Escherichia coli. The results confirmed that the blended films may be used as exceptional material for food packaging. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity

    Directory of Open Access Journals (Sweden)

    V. Gopinath

    2017-12-01

    Full Text Available The advanced research and development of silver nanoparticles (AgNPs is vast due to their incredible applications today. In this work, AgNPs were synthesized using soil derived Pseudomonas putida MVP2. The AgNPs formation on the P. putida cell membrane and its cell free supernatant was studied. The synthesized AgNPs were characterized by UV–visible spectroscopy, scanning transmission electron microscopy (STEM, X-ray diffraction (XRD, energy dispersive X-ray (EDAX and Fourier transform infrared (FTIR spectrum analysis. The mode of action of AgNPs on the bacteria was studied against clinically isolated bacterial pathogens, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Helicobacter pylori by membrane integrity, and protein leakage using confocal and electron microscopy. Interestingly, AgNPs had no cytotoxicity under 25 μg/mL and it was toxic at above 50 μg/mL on human epidermoid larynx carcinoma (HEp-2 cells. This study evidenced that biogenic nanoparticles could affect the bacterial replication, protein leakage and eventually cell death. This might be used for active antimicrobial agents for the chronic infections.

  19. Synthesis, characterization, and antibacterial activities of ZnLaFe2O4/NiTiO3 nanocomposite

    Science.gov (United States)

    Sobhani-Nasab, Ali; Zahraei, Zohreh; Akbari, Maryam; Maddahfar, Mahnaz; Hosseinpour-Mashkani, S. Mostafa

    2017-07-01

    In this research, for the first time, ZnLaFe2O4/NiTiO3 nanocomposites have been synthesized through a polyol assistant sol-gel method. To investigate the effect of different surfactants on the morphology and particle size of ZnLaFe2O4 nanostructure, cetrimonium bromide, sodium dodecyl sulfate, polyvinylpyrrolidone, polyvinyl alcohol, and oleic acid were used as surfactant agents. Based on the SEM results, it was found that morphology and particle size of the products could be affected by these surfactants. Furthermore, study on antibacterial effect of ZnLaFe2O4/NiTiO3 nanocomposites by colony forming unit (CFU) reduction assay showed that ZnLaFe2O4/NiTiO3 nanocomposites have antibacterial activity against Gram-negative Escherchia coli (ATCC 10536) and Gram-positive Staphylococcus aureus (ATCC 29737). Antibacterial results demonstrate that nanocomposite significantly reduced the growth rate of E. coli bacteria and S. aureus after 120 min. The structure and morphology of the resulting particles were characterized by XRD, FT-IR, EDX, and SEM analysis.

  20. SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ...

    African Journals Online (AJOL)

    B. S. Chandravanshi

    compounds. Tetradentate Schiff bases with a N2O2 donor atom set are well known to coordinate with various metal ions, and this has attracted many authors [17, 18]. Some Co(II), Cu(II) and Fe(II) Schiff base chelate complexes show catalytic activity in oxygenation of alkene and increase the rate of hydrolysis more than ...

  1. Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics

    Directory of Open Access Journals (Sweden)

    Singh R

    2013-11-01

    Full Text Available Richa Singh,1 Priyanka Wagh,1 Sweety Wadhwani,1 Sharvari Gaidhani,2 Avinash Kumbhar,3 Jayesh Bellare,4 Balu Ananda Chopade1 1Department of Microbiology, University of Pune, Pune, Maharashtra, India; 2Institute of Bioinformatics and Biotechnology, University of Pune, Pune, Maharashtra, India; 3Department of Chemistry, University of Pune, Pune, Maharashtra, India; 4Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India Background: The development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. The present study aims to biosynthesize silver nanoparticles (AgNPs using a cell-free extract of Acinetobacter spp. and evaluate their antibacterial activity. Methods: Eighteen strains of Acinetobacter were screened for AgNP synthesis. AgNPs were characterized using various techniques. Reaction parameters were optimized, and their effect on the morphology of AgNPs was studied. The synergistic potential of AgNPs on 14 antibiotics against seven pathogens was determined by disc-diffusion, broth-microdilution, and minimum bactericidal concentration assays. The efficacy of AgNPs was evaluated as per the minimum inhibitory concentration (MIC breakpoints of the Clinical and Laboratory Standards Institute (CLSI guidelines. Results: Only A. calcoaceticus LRVP54 produced AgNPs within 24 hours. Monodisperse spherical nanoparticles of 8–12 nm were obtained with 0.7 mM silver nitrate at 70°C. During optimization, a blue-shift in ultraviolet-visible spectra was seen. X-ray diffraction data and lattice fringes (d =0.23 nm observed under high-resolution transmission electron microscope confirmed the crystallinity of AgNPs. These AgNPs were found to be more effective against Gram-negative compared with Gram-positive microorganisms. Overall, AgNPs showed the highest synergy with vancomycin in the disc-diffusion assay. For Enterobacter

  2. Synthesis, characterizations and anti-bacterial activities of pure and Ag doped CdO nanoparticles by chemical precipitation method

    Science.gov (United States)

    Sivakumar, S.; Venkatesan, A.; Soundhirarajan, P.; Khatiwada, Chandra Prasad

    2015-02-01

    In the present study, synthesized pure and Ag (1%, 2%, and 3%) doped Cadmium Oxide (CdO) nanoparticles by chemical precipitation method. Then, the synthesized products were characterized by thermo gravimetric-differential thermal analysis (TG-DTA), X-ray diffraction (XRD) analysis, Fourier transform infrared (FT-IR) spectroscopy, Ultra violet-Vis diffused reflectance spectroscopy (UV-Vis-DRS), Scanning electron microscopy (SEM), Energy dispersive X-rays (EDX) spectroscopy, and anti-bacterial activities, respectively. The transition temperatures and phase transitions of Cd(OH)2 to CdO at 400 °C was confirmed by TG-DTA analysis. The XRD patterns show the cubic shape and average particle sizes are 21, 40, 34, and 37 nm, respectively for pure and Ag doped samples. FT-IR study confirmed the presence of CdO and Ag at 677 and 459 cm-1, respectively. UV-Vis-DRS study shows the variation on direct and indirect band gaps. The surface morphologies and elemental analysis have been confirmed from SEM and with EDX. In addition, the synthesized products have been characterized by antibacterial activities against Gram-positive and negative bacteria. Further, the present investigation suggests that CdO nanoparticles have the great potential applications on various industrial and medical fields of research.

  3. Shape and size controlled synthesis of Au nanorods: H2S gas-sensing characterizations and antibacterial application

    International Nuclear Information System (INIS)

    Lanh, Le Thi; Hoa, Tran Thai; Cuong, Nguyen Duc; Khieu, Dinh Quang; Quang, Duong Tuan; Van Duy, Nguyen; Hoa, Nguyen Duc; Van Hieu, Nguyen

    2015-01-01

    Highlights: • We have demonstrated a facile method to prepare colloid Au nanorods. • The size and shape of Au nanorods can be controlled via seed-mediated growth method. • The H 2 S gas-sensing properties have been investigated. • The antibacterial application has been conducted. - Abstract: Controlling their size and shape is one of the important issues in the fundamental study and application of colloidal metal nanoparticles. In the current study, different sizes and shapes of Au nanorods were fabricated using a seed-mediated growth method. Material characterization by X-ray diffraction and transmission electron microscopy revealed that the obtained products were made of single-crystal Au nanorods with an average diameter and length of 10 nm and 40 nm, respectively. The Au nanorod-based sensor exhibited significantly high sensitivity and fast response/recovery time to low concentrations (2.5–10 ppm) of H 2 S at temperatures ranging from 300 °C to 400 °C. Additionally, they exhibited antibacterial effect at low concentration. These results suggested that the fabricated Au nanorods have excellent potential for practical application in air pollution monitoring and biomedicine

  4. Shape and size controlled synthesis of Au nanorods: H{sub 2}S gas-sensing characterizations and antibacterial application

    Energy Technology Data Exchange (ETDEWEB)

    Lanh, Le Thi [College of Sciences, Hue University, 77 Nguyen Hue, Hue City (Viet Nam); Hoa, Tran Thai, E-mail: trthaihoa@yahoo.com [College of Sciences, Hue University, 77 Nguyen Hue, Hue City (Viet Nam); Cuong, Nguyen Duc [College of Sciences, Hue University, 77 Nguyen Hue, Hue City (Viet Nam); Faculty of Hospitality and Tourism, Hue University, 22 Lam Hoang, Hue City (Viet Nam); Khieu, Dinh Quang [College of Sciences, Hue University, 77 Nguyen Hue, Hue City (Viet Nam); Quang, Duong Tuan [College of Education, Hue University, 34 Le Loi, Hue City (Viet Nam); Van Duy, Nguyen; Hoa, Nguyen Duc [International Training Institute for Materials Science, Hanoi University of Science and Technology, Hanoi (Viet Nam); Van Hieu, Nguyen, E-mail: hieu@itims.edu.vn [International Training Institute for Materials Science, Hanoi University of Science and Technology, Hanoi (Viet Nam)

    2015-06-25

    Highlights: • We have demonstrated a facile method to prepare colloid Au nanorods. • The size and shape of Au nanorods can be controlled via seed-mediated growth method. • The H{sub 2}S gas-sensing properties have been investigated. • The antibacterial application has been conducted. - Abstract: Controlling their size and shape is one of the important issues in the fundamental study and application of colloidal metal nanoparticles. In the current study, different sizes and shapes of Au nanorods were fabricated using a seed-mediated growth method. Material characterization by X-ray diffraction and transmission electron microscopy revealed that the obtained products were made of single-crystal Au nanorods with an average diameter and length of 10 nm and 40 nm, respectively. The Au nanorod-based sensor exhibited significantly high sensitivity and fast response/recovery time to low concentrations (2.5–10 ppm) of H{sub 2}S at temperatures ranging from 300 °C to 400 °C. Additionally, they exhibited antibacterial effect at low concentration. These results suggested that the fabricated Au nanorods have excellent potential for practical application in air pollution monitoring and biomedicine.

  5. Synthesis, spectroscopic characterization and antibacterial studies of lanthanide(III) Schiff base complexes containing N, O donor atoms

    Science.gov (United States)

    Lekha, L.; Raja, K. Kanmani; Rajagopal, G.; Easwaramoorthy, D.

    2014-01-01

    A series of six Ln(III) Schiff base complexes, Pr(III), Sm(III), Gd(III), Tb(III), Er(III) and Yb(III), were synthesized using sodium salt of Schiff base, 2-[(5-bromo-2-hydroxy-benzylidene)-amino]-3-hydroxy-propionic acid, derived from L-serine and 5-bromosalicylaldehyde. These complexes having general formula [Ln(L)(NO3)2(H2O)]·NO3 were characterized by elemental analysis, conductivity measurements, UV-Vis, FT-IR, mass spectrometry and fluorescence studies. Elemental analysis and conductivity measurements suggest the complexes have a 1:1 stoichiometry. From the spectral studies it has been concluded that Ln(III) complexes display eight coordination. The Schiff base and its Ln(III) metal complexes have also been screened for their antibacterial activities by Agar diffusion method.

  6. Mixed-Ligand Nickel(II Complexes Containing Sulfathiazole and Cephalosporin Antibiotics: Synthesis, Characterization, and Antibacterial Activity

    Directory of Open Access Journals (Sweden)

    J. R. Anacona

    2012-01-01

    (L1,4, x=1; L2,3, x=0; L = monoanion of cefazolin HL1, cephalothin HL2, cefotaxime HL3, ceftriaxone HL4 and [Ni(L5(stz]Cl (cefepime L5, which were characterized by physicochemical and spectroscopic methods. Their spectra indicated that cephalosporins are acting as multidentate chelating agents, via the lactam carbonyl and carboxylate and N-azomoieties. The complexes are insoluble in water and common organic solvents but soluble in DMSO, where the [Ni(L5(stz]Cl complex is 1 : 1 electrolyte. They probably have polymeric structures. They have been screened for antibacterial activity, and the results are compared with the activity of commercial cephalosporins.

  7. Synthesis and characterization of silver/montmorillonite/chitosan bionanocomposites by chemical reduction method and their antibacterial activity

    Directory of Open Access Journals (Sweden)

    Kamyar Shameli

    2011-01-01

    Full Text Available Kamyar Shameli1, Mansor Bin Ahmad1, Mohsen Zargar3, Wan Md Zin Wan Yunus1, Nor Azowa Ibrahim1, Parvaneh Shabanzadeh2, Mansour Ghaffari Moghaddam41Department of Chemistry, Faculty of Science, 2Institute for Mathematical Research, Universiti Putra Malaysia, Selangor, Malaysia; 3Department of Biology, Islamic Azad University, Qum, Iran; 4Department of Chemistry, Faculty of Science, University of Zabol, Zabol, IranAbstract: Silver nanoparticles (AgNPs of a small size were successfully synthesized using the wet chemical reduction method into the lamellar space layer of montmorillonite/chitosan (MMT/Cts as an organomodified mineral solid support in the absence of any heat treatment. AgNO3, MMT, Cts, and NaBH4 were used as the silver precursor, the solid support, the natural polymeric stabilizer, and the chemical reduction agent, respectively. MMT was suspended in aqueous AgNO3/Cts solution. The interlamellar space limits were changed (d-spacing = 1.24–1.54 nm; therefore, AgNPs formed on the interlayer and external surface of MMT/Cts with d-average = 6.28–9.84 nm diameter. Characterizations were done using different methods, ie, ultraviolet-visible spectroscopy, powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray fluorescence spectrometry, and Fourier transform infrared spectroscopy. Silver/montmorillonite/chitosan bionanocomposite (Ag/MMT/Cts BNC systems were examined. The antibacterial activity of AgNPs in MMT/Cts was investigated against Gram-positive bacteria, ie, Staphylococcus aureus and methicillin-resistant S. aureus and Gram-negative bacteria, ie, Escherichia coli, E. coli O157:H7, and Pseudomonas aeruginosa by the disc diffusion method using Mueller Hinton agar at different sizes of AgNPs. All of the synthesized Ag/MMT/Cts BNCs were found to have high antibacterial activity. These results show that Ag/MMT/Cts BNCs can be useful in different biological research and biomedical

  8. Synthesis and Characterization of Monometallic (Ag, Cu and Bimetallic Ag-Cu Particles for Antibacterial and Antifungal Applications

    Directory of Open Access Journals (Sweden)

    Marta Paszkiewicz

    2016-01-01

    Full Text Available In this paper, the experimental studies are concerned with the effect of the synthesis parameters on the formation of monometallic Ag and Cu nanoparticles (NPs. We consider the synthesis strategies verification for the bimetallic core-shell and alloy particles preparation. It was successfully obtained by chemical reduction method. The obtained colloidal solution is characterized by the transmission electron microscopy (TEM with energy-dispersive X-ray spectroscopy (EDX data, UV-Vis spectra, particle size distribution, and zeta potential. This work presents a comprehensive overview of experimental studies of the most stable colloidal solutions to impregnate fabrics that will exhibit a bactericidal and fungicidal activity against Candida albicans, Escherichia coli, and Staphylococcus aureus.

  9. Unsymmetrical Schiff base (ON) ligand on complexation with some transition metal ions: synthesis, spectral characterization, antibacterial, fluorescence and thermal studies.

    Science.gov (United States)

    Ali, Omyma A M; El-Medani, Samir M; Abu Serea, Maha R; Sayed, Abeer S S

    2015-02-05

    A series of eight metal Schiff base complexes were synthesized by the thermal reaction of Cu(II), Ni(II), Fe(III), Co(II), Zn(II), Hg(II), La(III) or Sm(III) with a Schiff base "L" produced by the condensation of furfuraldehyde and 1,2-diaminobenzene. These compounds were characterized by elemental analysis, UV-Vis, FT-IR, molar conductance, mass spectrometry, thermal and fluorescence studies. The studies suggested the coordination of the ligand L to metal through azomethine imine nitrogen and furan oxygen atoms of Schiff base moiety. Thermogravimetric (TG/DTG) analyses data were studied and indicated high stability for all complexes and suggested the presence of lattice and/or coordinated water molecules in the complexes. Coats-Redfern method has been used to calculate the kinetic and thermodynamic parameters of the metal complexes. The spectral and thermal analysis reveal that all complexes have octahedral geometry except Cu(II) and Ni(II) complexes which can attain a square planner arrangements. The ligand and its complexes exhibited intraligand (π-π(∗)) fluorescence and can potentially serve as photoactive materials. Both the ligand and its complexes have been screened for antibacterial activities. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Synthesis, characterization, antibacterial and antifungal studies of some transition and rare earth metal complexes of N-benzylidene-2-hydroxybenzohydrazide

    Directory of Open Access Journals (Sweden)

    T.K. Chondhekar

    2011-12-01

    Full Text Available The solid complexes of Cu(II, Co(II, Mn(II, La(III and Ce(III were prepared from bidentate Schiff base, N-benzylidene-2-hydroxybenzohydrazide. The Schiff base ligand was synthesized from 2-hyhdroxybenzohydrazide and benzaldehyde. These metal complexes were characterized by molar conductivity, magnetic susceptibility, thermal analysis, X-ray diffraction, FTIR, 1H-NMR, UV-Vis and mass spectroscopy. The analytical data of these metal complexes showed metal:ligand ratio of 1:2. The physico-chemical study supports the presence of square planar geometry around Cu(II and octahedral geometry around Mn(II, Co(II, La(III and Ce(III ions. The IR spectral data reveal that the ligand behaves as bidentate with ON donor atom sequence towards central metal ion. The molar conductance values of metal complexes suggest their non-electrolyte nature. The X-ray diffraction data suggest monoclinic crystal system for these complexes. Thermal behavior (TG/DTA and kinetic parameters calculated by Coats-Redfern method are suggestive of more ordered activated state in complex formation. The ligand and their metal complexes were screened for antibacterial activity against Staphylococcus aureus and Escherichia coli and fungicidal activity against Aspergillus niger and Trichoderma.

  11. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu–Ni nanoparticles for potential use in dental materials

    Directory of Open Access Journals (Sweden)

    Liliana Argueta-Figueroa

    2014-08-01

    Full Text Available The antibacterial effect is a desirable property in dental materials. Development of simple methods for the preparation of nanosized metal particles has attracted significant attention because of their future applications due to unusual size-dependent antibacterial properties. Copper (Cu, Nickel (Ni and bimetallic Cu–Ni nanoparticles were prepared by a simple chemical method and their antibacterial activity was tested against the widely used standard human pathogens Staphylococcus aureus (gram-negative and Escherichia coli (gram-positive. Additionally, these nanoparticles were tested against the dental pathogen Streptococcus mutans. Our results are promising for potential use in dental materials science.

  12. Synthesis and Characterization of Silver Nanoparticles-Filled Polyethersulfone Membranes for Antibacterial and Anti-Biofouling Application.

    Science.gov (United States)

    Rehan, Zulfiqar Ahmad; Gzara, Lassaad; Khan, Sher Bahadar; Alamry, Khalid A; El-Shahawi, M S; Albeirutty, Mohammad H; Figoli, Alberto; Drioli, Enrico; Asiri, Abdullah M

    2016-01-01

    The membrane processes are interesting and economical techniques for reuse of municipal and industrial wastewater as well as seawater desalination. However their drawbacks can be resumed in the fouling and biofouling due to the deposition and adsorption phenomenon of the components and the development of biofilm on membrane surface. Several studies have focused on the effect of the incorporation of nanoparticles in polymeric membrane matrix on the biofouling properties. Few relevant patents to the topic have been reviewed and cited. Polyethersulfone (PES) membranes filled with silver nanoparticles (AgNPs) were prepared by non-solvent induced phase separation (NIPS) process using polyvinylpyrrolidone (PVP) as additive and N-Methyl-2-pyrrolidone (NMP) as solvent. Dope solution compositions, coagulation bath (CB) compositions, time before immersion in CB and casting speed were systematically studied. Membrane structure was characterized by scanning electron microscopy, contact angle, streaming potential measurement and X-ray diffraction (XRD). Membrane performance was assessed by pure water permeability, antifouling property, porosity and mechanical property. Silver nanoparticles (AgNPs) were prepared by the chemical reduction of silver nitrate solution with freshly prepared fructose solution, using PVP as capping agent and NaOH as accelerant and settled using acetone. The synthesized AgNPs were firstly characterized by Dynamic light scattering (DLS) technique, UV-visible spectrophotometer and X-ray diffraction spectroscopy (XRD). Then, we have selected a 15% PES mixed with 15% of PVP dope solution to prepare PESAgNPs blended membranes. All the nanocomposite membranes showed superb antibacterial and anti-biofouling performances, indicating that AgNPs in the PES membranes could be an effective approach to minimize membrane biofouling.

  13. Synthesis, spectroscopic characterization, crystallographic studies and antibacterial assays of new copper(II) complexes with sulfathiazole and nimesulide

    Science.gov (United States)

    Nunes, Julia Helena Bormio; de Paiva, Raphael Enoque Ferraz; Cuin, Alexandre; da Costa Ferreira, Ana Maria; Lustri, Wilton Rogério; Corbi, Pedro Paulo

    2016-05-01

    New ternary copper(II) complexes of sulfathiazole (SFT, C9H8N3O2S2) or nimesulide (NMS, C13H11N2O5S) and 2,2‧-bipyridine (bipy) were synthesized, and characterized by chemical and spectroscopic techniques. Elemental analyses indicated a 2:1:1 sulfonamide/copper/bipy composition for both complexes. Mass spectrometric measurements permitted identifying the molecular ions [Cu(SFT)2(bipy)+H]+ and [Cu(NMS)2(bipy)+H]+ at m/z 728 and 835, respectively, confirming the proposed compositions. Crystal structure of the [Cu(SFT)2(bipy)] complex was solved by powder X-ray diffraction analysis (PXRD), attesting that the Cu(II) ion is hexacoordinated in a distorted octahedral geometry. Each SFT molecule coordinates to the metal ion by the nitrogen atoms of the SO2-N group and of the heterocyclic ring. The coordination sphere is completed by a bipyridine. Electronic paramagnetic resonance (EPR) studies were carried out for the [Cu(NMS)2(bipy)] complex, indicating a tetragonal environment around the metal ion. It was suggested that NMS coordinates to Cu(II) by the nitrogen and oxygen atoms of the SO2-N group, which was confirmed by infrared spectroscopic studies. Biological studies showed the antibacterial activity of both Cu-SFT and Cu-NMS complexes, with the minimum inhibitory concentration (MIC) values ranging from 0.10 to 0.84 mmol L-1 against Gram-negative bacteria for [Cu(SFT)2(bipy)], and from 1.50 to 3.00 mmol L-1 against Gram-positive and -negative bacteria for [Cu(NMS)2(bipy)].

  14. Synthesis, characterization, nano-sized binuclear nickel complexes, DFT calculations and antibacterial evaluation of new macrocyclic Schiff base compounds

    Science.gov (United States)

    Parsaee, Zohreh; Mohammadi, Khosro

    2017-06-01

    Some new macrocyclic bridged dianilines tetradentate with N4coordination sphere Schiff base ligands and their nickel(II)complexes with general formula [{Ni2LCl4} where L = (C20H14N2X)2, X = SO2, O, CH2] have been synthesized. The compounds have been characterized by FT-IR, 1H and 13C NMR, mass spectroscopy, TGA, elemental analysis, molar conductivity and magnetic moment techniques. Scanning electron microscopy (SEM) shows nano-sized structures under 100 nm for nickel (II) complexes. NiO nanoparticle was achieved via the thermal decomposition method and analyzed by FT-IR, SEM and X-ray powder diffraction which indicates closeaccordance to standard pattern of NiO nanoparticle. All the Schiff bases and their complexes have been detected in vitro both for antibacterial activity against two gram-negative and two gram-positive bacteria. The nickel(II) complexes were found to be more active than the free macrocycle Schiff bases. In addition, computational studies of three ligands have been carried out at the DFT-B3LYP/6-31G+(d,p) level of theory on the spectroscopic properties, including IR, 1HNMR and 13CNMR spectroscopy. The correlation between the theoretical and the experimental vibrational frequencies, 1H NMR and 13C NMR of the ligands were 0.999, 0.930-0.973 and 0.917-0.995, respectively. Also, the energy gap was determined and by using HOMO and LUMO energy values, chemical hardness-softness, electronegativity and electrophilic index were calculated.

  15. SHORT COMMUNICATION SYNTHESIS AND ANTIBACTERIAL ...

    African Journals Online (AJOL)

    Admin

    a. sodium nitrite, b. ethylacetoacetate, c. phenyl hydrazine, d. hydrazine hydrate, e. hydroxyl- amine hydrochloride, f. Salycylaldehye, g. chloroacetyl chloride, h. thioglycollic acid. Scheme 1. Synthesis of heterocyclic derivatives of sulfanilamide. Synthesis of compound 1 and 2a-e were done as per the reported method [7-8] ...

  16. Synthesis, characterization, and evaluation of poly(aminoethyl) modified chitosan and its hydrogel used as antibacterial wound dressing.

    Science.gov (United States)

    Zhang, Yubei; Dang, Qifeng; Liu, Chengsheng; Yan, Jingquan; Cha, Dongsu; Liang, Shengnan; Li, Xiaoli; Fan, Bing

    2017-09-01

    This study aims to develop new antibacterial hydrogel wound dressings composed of poly(aminoethyl) modified chitosan (PAEMCS). FTIR, 1 H NMR, and elemental analysis demonstrated that PAEMCS was successfully synthesized via grafting poly(aminoethyl) groups onto hydroxyl groups on chitin first, and removing acetyl groups from the grafted polymer afterward. XRD and TGA implied its well-defined crystallinity and thermostability. Furthermore, a series of hydrogels were fabricated under the participation of dipotassium hydrogen phosphate (DHP). The gelation tests suggested that the higher concentration of PAEMCS or DHP was beneficial to the formation of hydrogels. The pH values of hydrogels at 37°C were all in the range of 7.12-7.50. The rheological tests indicated that PAEMCS-based hydrogels were of lower DHP addition and higher elasticity than CS-based hydrogels to achieve the same gelation temperature under the same polymer's concentration. Additionally, the swelling, anti-bacteria, and cytotoxicity experiments showed that PAEMCS-based hydrogels possessed excellent hygroscopicity, high antibacterial activity against E. coli, S. aureus, or S. epidermidis, and good cytocompatibility toward L929 cells or HUVECs, respectively. All the results implied that PAEMCS-based hydrogels not only maintained inherent multiple properties of chitosan but also possessed excellent antibacterial activity, and might be promising antibacterial hydrogel dressings used in wound therapy. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Synthesis, characterization and antibacterial properties of a novel nanocomposite based on polyaniline/polyvinyl alcohol/Ag

    Directory of Open Access Journals (Sweden)

    Mansour Ghaffari-Moghaddam

    2014-11-01

    Full Text Available In this study, a novel nanocomposite based on polyaniline/polyvinyl alcohol/Ag (PANI/PVA/Ag has been successfully synthesized. The chemical reduction method was used to produce Ag nanoparticle colloidal solution from Ag+ ions. The polymerization of aniline occurred in situ for the preparation of polyaniline (PANI in the presence of ammonium persulfate. With exposure to Ag nanoparticles on the PANI/PVA composite, a new nanocomposite was obtained. The morphology and particle size of the novel nanocomposite was studied by scanning electron microscopy (SEM, X-ray diffraction (XRD, and Fourier transform infrared (FT-IR analyses. According to XRD analysis, the size of nanoparticles was found to be in the range of 10–17 nm. SEM images showed the favored shape of nanoparticles as triangle which is a benign shape for antibacterial analysis. The antibacterial activity of the obtained nanocomposite was also evaluated against Gram positive bacteria Staphylococcus aureus (Staph. aureus and Gram negative Escherichia coli (E. coli using the paper disk diffusion method. The antibacterial study showed that the PANI/PVA composite did not have a very good antibacterial activity but PANI/PVA/Ag nanocomposites were found to be effective against two bacteria.

  18. Synthesis, characterization and thermal behavior of antibacterial and antifungal active zinc complexes of bis (3(4-dimethylaminophenyl)-allylidene-1,2-diaminoethane

    Energy Technology Data Exchange (ETDEWEB)

    Montazerozohori, Morteza, E-mail: mmzohori@mail.yu.ac.ir [Department of Chemistry, Yasouj University, Yasouj 75918-74831 (Iran, Islamic Republic of); Zahedi, Saeedeh [Department of Chemistry, Yasouj University, Yasouj 75918-74831 (Iran, Islamic Republic of); Naghiha, Asghar [Department of Animal Sciences, Faculty of Agriculture, Yasouj University, Yasouj (Iran, Islamic Republic of); Zohour, Mostafa Montazer [Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan (Iran, Islamic Republic of)

    2014-02-01

    In this work, synthesis of a new series of zinc halide/pseudohalide complexes of a bidentate Schiff base ligand entitled as bis (3-(4-dimethylaminophenyl)-allylidene)-1,2-diaminoethane(L) is described. The ligand and its zinc complexes were characterized by various techniques such as elemental analysis, FT-IR, UV–visible, {sup 1}H and {sup 13}C NMR spectra, cyclic voltammetry, and conductometry. Accordingly ZnLX{sub 2} (X = Cl{sup −}, Br{sup −}, I{sup −}, SCN{sup −} and N{sub 3}{sup −}) was suggested as molecular formula of the complexes. Redox behaviors of ligand and its zinc complexes were investigated by cyclic voltammetry method. Furthermore, the ligand and its zinc halide/pseudohalide complexes were tested for their in vitro antibacterial activities against two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Also in vitro antifungal activities of them against Candida albicans and Aspergillus niger were investigated. The results indicated that all compounds are antibacterial and antifungal active. Thermal behaviors of ligand and its zinc complexes were studied from room temperature to 1100 °C under argon atmosphere. It was found that the ligand and zinc iodide are decomposed completely via three and four steps respectively while other zinc complexes leave out the metal or organometallic compounds as final residuals after 3–4 decomposition steps at above temperature range. Moreover evaluation of some thermo-kinetic parameters such as activation energy (∆E{sup ⁎}), enthalpy (∆H{sup ⁎}), entropy (∆S{sup ⁎}) and Gibbs free energy change (∆G{sup ⁎}) of the thermal decomposition steps were performed based on the Coats–Redfern relation. - Highlights: • Some novel complexes of Zn(II) with a bidentate Schiff base ligand have been synthesized. • Redox behavior of ligand and zinc complexes was investigated by cyclic voltammetry. • The

  19. Synthesis, characterization and thermal behavior of antibacterial and antifungal active zinc complexes of bis (3(4-dimethylaminophenyl)-allylidene-1,2-diaminoethane

    International Nuclear Information System (INIS)

    Montazerozohori, Morteza; Zahedi, Saeedeh; Naghiha, Asghar; Zohour, Mostafa Montazer

    2014-01-01

    In this work, synthesis of a new series of zinc halide/pseudohalide complexes of a bidentate Schiff base ligand entitled as bis (3-(4-dimethylaminophenyl)-allylidene)-1,2-diaminoethane(L) is described. The ligand and its zinc complexes were characterized by various techniques such as elemental analysis, FT-IR, UV–visible, 1 H and 13 C NMR spectra, cyclic voltammetry, and conductometry. Accordingly ZnLX 2 (X = Cl − , Br − , I − , SCN − and N 3 − ) was suggested as molecular formula of the complexes. Redox behaviors of ligand and its zinc complexes were investigated by cyclic voltammetry method. Furthermore, the ligand and its zinc halide/pseudohalide complexes were tested for their in vitro antibacterial activities against two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Also in vitro antifungal activities of them against Candida albicans and Aspergillus niger were investigated. The results indicated that all compounds are antibacterial and antifungal active. Thermal behaviors of ligand and its zinc complexes were studied from room temperature to 1100 °C under argon atmosphere. It was found that the ligand and zinc iodide are decomposed completely via three and four steps respectively while other zinc complexes leave out the metal or organometallic compounds as final residuals after 3–4 decomposition steps at above temperature range. Moreover evaluation of some thermo-kinetic parameters such as activation energy (∆E ⁎ ), enthalpy (∆H ⁎ ), entropy (∆S ⁎ ) and Gibbs free energy change (∆G ⁎ ) of the thermal decomposition steps were performed based on the Coats–Redfern relation. - Highlights: • Some novel complexes of Zn(II) with a bidentate Schiff base ligand have been synthesized. • Redox behavior of ligand and zinc complexes was investigated by cyclic voltammetry. • The ligand and its zinc complexes are antibacterial and

  20. Synthesis and antibacterial activity screening of quaternary ...

    Indian Academy of Sciences (India)

    A series of quaternary ammonium derivatives of triazolyl pyranochromen-2-ones have been synthesized and characterized; their antibacterial potential were investigated against two gram negative (Pseudomonas aeruginosa and Escherichia coli) and two gram positive bacterial strains (Bacillus cereus and Staphylococcus ...

  1. Synthesis, characterization and antibacterial activity against Gram positive and Gram negative bacteria of biomimetically coated silver nanoparticles.

    Science.gov (United States)

    Amato, Elvio; Diaz-Fernandez, Yuri A; Taglietti, Angelo; Pallavicini, Piersandro; Pasotti, Luca; Cucca, Lucia; Milanese, Chiara; Grisoli, Pietro; Dacarro, Cesare; Fernandez-Hechavarria, Jose M; Necchi, Vittorio

    2011-08-02

    In the present work, we describe a simple procedure to produce biomimetically coated silver nanoparticles (Ag NPs), based on the postfunctionalization and purification of colloidal silver stabilized by citrate. Two biological capping agents have been used (cysteine Cys and glutathione GSH). The composition of the capped colloids has been ascertained by different techniques and antibacterial tests on GSH-capped Ag NPs have been conducted under physiological conditions, obtaining values of Minimum Inhibitory Concentration (MIC) of 180 and 15 μg/mL for Staphylococcus aureus and Escherichia coli, respectively. The antibacterial activity of these GSH capped NPs can be ascribed to the direct action of metallic silver NPs, rather than to the bulk release of Ag(+).

  2. Synthesis and characterization of anti-bacterial and anti-fungal citrate-based mussel-inspired bioadhesives

    Science.gov (United States)

    Guo, Jinshan; Wang, Wei; Hu, Jianqing; Xie, Denghui; Gerhard, Ethan; Nisic, Merisa; Shan, Dingying; Qian, Guoying; Zheng, Siyang; Yang, Jian

    2016-01-01

    Bacterial and fungal infections in the use of surgical devices and medical implants remain a major concern. Traditional bioadhesives fail to incorporate anti-microbial properties, necessitating additional anti-microbial drug injection. Herein, by the introduction of the clinically used and inexpensive anti-fungal agent, 10-undecylenic acid (UA), into our recently developed injectable citrate-based mussel-inspired bioadhesives (iCMBAs), a new family of anti-bacterial and anti-fungal iCMBAs (AbAf iCs) was developed. AbAf iCs not only showed strong wet tissue adhesion strength, but also exhibited excellent in vitro cyto-compatibility, fast degradation, and strong initial and considerable long-term anti-bacterial and anti-fungal ability. For the first time, the biocompatibility and anti-microbial ability of sodium metaperiodate (PI), an oxidant used as a cross-linking initiator in the AbAf iCs system, was also thoroughly investigated. Our results suggest that the PI-based bioadhesives showed better anti-microbial properties compared to the unstable silver-based bioadhesive materials. In conclusion, AbAf iCs family can serve as excellent anti-bacterial and anti-fungal bioadhesive candidates for tissue/wound closure, wound dressing, and bone regeneration, especially when bacterial or fungal infections are a major concern. PMID:26874283

  3. Microwave synthesis and antibacterial studies of bioceramics doped with antibacterial metal

    Science.gov (United States)

    Iqbal, Nida; Kadir, M. R. Abdul

    2017-12-01

    The aim of this work was to evaluate the effect of zinc metal ion on the antibacterial properties of bioceramics i.e. hydroxyapatite for the protection of bacterial infections. In this paper, rapid icrowave synthesis of hydroxyapatite with different weightages of antibacterial ions zinc (2 and 4 wt%) at 850 watts via microwave irradiation method at 10 mins was reported. The synthesized bioceramics were chemically characterized using energy dispersive X-ray (EDAX) and Fourier transform infrared spectroscopy (FTIR) studies. FT-IR analysis revealed the presence of Zn into HA lattices due to the increasing in the vibrational modes corresponds to phosphates and hydroxyl groups and EDAX analysis confirmed the presence of oxygen (O), calcium (Ca), phosphor (P), and zinc (Zn) in the ZnHA samples. Antibacterial studies have demonstrated that each of the Zn-bearing bioceramics samples exhibits marked antibacterial effects against Bacillus subtilis and Pseudomonas aeruginosa. Based on the results, it can be concluded that the synthesized Zn HA bioceramics have potential to prevent the bacterial infections and can be used as bone tissue regeneration.

  4. Synthesis, structural characterization and antibacterial activity of cotton fabric modified with a hydrogel containing barium hexaferrite nanoparticles

    Science.gov (United States)

    Staneva, Desislava; Koutzarova, Tatyana; Vertruyen, Benedicte; Vasileva-Tonkova, Evgenia; Grabchev, Ivo

    2017-01-01

    Barium hexaferrite nanoparticles were synthesized by co-precipitation of Ba2+ and Fe3+ cations with NaOH under of high-power ultrasound. The nanoparticles were dispersed in an aqueous solution of the hydrogel precursors. This solution was used to impregnate the cotton fabric dyed with a photoinitiator. The composite material BaFe12O19 nanoparticles-hydrogel-cotton fabric was prepared by surface initiate photopolymerization under visible light. The modification of the cotton fabric and uniform distribution of the nanoparticles in the structure of the hydrogel were analyzed by scanning electron microscopy (SEM), IR spectroscopy, X-ray diffraction analysis (XRD), fluorescence and colourimetric analyses. The antibacterial efficacy of the material was evaluated against Gram-negative Escherichia coli and Pseudomonas aeruginosa.

  5. Synthesis and Antibacterial Activity of Antibiotic-Functionalized Graphite Nanofibers

    Directory of Open Access Journals (Sweden)

    Madeline Rotella

    2015-01-01

    Full Text Available Surface functionalization of nanomaterials is an area of current investigation that supports the development of new biomaterials for applications in biology and medicine. Herein we describe the synthesis, characterization, and antibacterial properties of the first examples of antibiotic-labeled graphitic carbon nanofibers (GCNFs covalently functionalized with aminoglycoside and quinolone antibiotics. Ruthenium tetroxide oxidation of herringbone GCNFs gave higher amounts of surface carboxyl groups than previous methods. These carboxyl groups served as sites of attachment for antibiotics by acyl substitution. Bioassay of these novel, functionalized GCNFs using serial dilution and optical density methods demonstrated that antibiotic-labeled GCNFs possess significant antibacterial activity against Pseudomonas aeruginosa. The activity we observe for aminoglycoside-functionalized GCNFs suggests a membranolytic mechanism of action.

  6. Green synthesis and characterization of silver nanoparticles using alcoholic flower extract of Nyctanthes arbortristis and in vitro investigation of their antibacterial and cytotoxic activities

    Energy Technology Data Exchange (ETDEWEB)

    Gogoi, Nayanmoni [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Babu, Punuri Jayasekhar [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Mahanta, Chandan [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India); Bora, Utpal, E-mail: ubora@iitg.ernet.in [Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam (India); Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039 (India)

    2015-01-01

    Here we report the synthesis of silver nanoparticles using ethanolic flower extract of Nyctanthes arbortristis, UVvisible spectra and TEM indicated the successful formation of silver nanoparticles. Crystalline nature of the silver nanoparticles was confirmed by X-ray diffraction. Fourier Transform Infra-Red Spectroscopy analysis established the capping of the synthesized silver nanoparticles with phytochemicals naturally occurring in the ethanolic flower extract of N. arbortristis. The synthesized silver nanoparticles showed antibacterial activity against the pathogenic strain of Escherichia coli MTCC 443. Furthermore, cytotoxicity of the silver nanoparticles was tested on mouse fibroblastic cell line (L929) and found to be non-toxic, which thus proved their biocompatibility. Antibacterial activity and cytotoxicity assay carried out in this study open up an important perspective of the synthesized silver nanoparticles. - Highlights: • The present study depicts the green synthesis of AgNPs using Nyctanthes arbortristis. • AuNPs found to be biocompatible and can be used for biomedical applications. • The FTIR, TGA and DTA results showed that AgNPs are bounded by organic coating. • The synthesized AgNPs showed antibacterial activity on E. Coli MTCC 443. • We investigated the antioxidant activity for both EFE and AgNPs.

  7. Purification, Characterization and Antibacterial Mechanism of ...

    African Journals Online (AJOL)

    Purpose: To carry out the extraction, purification and biological characterization, and assess the antibacterial activity of bacteriocin from Lactobacillus acidophilus XH1. Methods: Chloroform extraction method was used for bacteriocin extraction while characterization of bacteriocin was carried out by flat-dug well agar ...

  8. Synthesis and characterization of the NiFe{sub 2}O{sub 4}@TEOS–TPS@Ag nanocomposite and investigation of its antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Allafchian, Ali R., E-mail: Allafchian@cc.iut.ac.ir [Nanotechnology and Advanced Materials Institute, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Jalali, S.A.H., E-mail: ahjalali2002@gmail.com [Department of Natural Resources, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Amiri, R., E-mail: razieh.amiri@gmail.com [Nanotechnology and Advanced Materials Institute, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of); Shahabadi, Sh., E-mail: shirinshahabadi@rocketmail.com [Nanotechnology and Advanced Materials Institute, Isfahan University of Technology, Isfahan 84156-83111 (Iran, Islamic Republic of)

    2016-11-01

    Highlights: • The new NiFe{sub 2}O{sub 4}@TEOS–TPS@Ag nanocomposite was synthesized and characterized. • The VSM technique was applied for investigation of their magnetic properties. • By using magnetic decantation, they can be easily removed from the disinfected media. • These nanocomposites exhibit good antibacterial activity and high reusability. - Abstract: In this study, the NiFe{sub 2}O{sub 4} was embedded in (3–mercaptopropyl) trimethoxysilane (TPS) and tetraethyl orthosilicate (TEOS) using the sol–gel method. These compounds were used as the support of Ag nanoparticles (Ag NPs). The NiFe{sub 2}O{sub 4}@TEOS–TPS@Ag nanocomposites were obtained with the development of bonding between the silver atoms of Ag NPs and the sulfur atoms of TPS molecule. Field emission scanning electron microscopy (FE–SEM), transmission electron microscopy (TEM), X–ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT–IR) were used for the characterization of the Ag nanocomposites. Also, the magnetic properties of these nanocomposites were studied by using a vibrating sample magnetometer (VSM) technique. The disk diffusion, minimum inhibition concentration (MIC) and minimum bactericidal concentrations (MBC) tests were used for the investigation of the antibacterial effect of this nanocomposite against bacterial strains. The synthesized nanocomposite presented high reusability and good antibacterial activity against gram–positive and gram–negative bacteria. Remarkably, this nanocomposite could be easily removed from the disinfected media by magnetic decantation.

  9. Synthesis, characterization and antibacterial activity of a tridentate Schiff base derived from cephalothin and sulfadiazine, and its transition metal complexes

    Science.gov (United States)

    Anacona, J. R.; Noriega, Natiana; Camus, Juan

    2015-02-01

    Metal(II) coordination compounds of a cephalothin Schiff base (H2L) derived from the condensation of cephalothin antibiotic with sulfadiazine were synthesized. The Schiff base ligand, mononuclear [ML(H2O)3] (M(II) = Mn, Co, Ni, Zn) complexes and magnetically diluted dinuclear copper(II) complex [CuL(H2O)3]2 were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and 1H NMR spectral studies. The cephalothin Schiff base ligand H2L behaves as a dianionic tridentate NOO chelating agent. The biological applications of complexes have been studied on two bacteria strains (Escherichia coli and Staphylococcus aureus) by agar diffusion disc method.

  10. Synthesis, characterization and anti-bacterial activity of divalent transition metal complexes of hydrazine and trimesic acid

    Directory of Open Access Journals (Sweden)

    K. Kumar

    2018-01-01

    Full Text Available Transition metal complexes of trimesic acid and hydrazine mixed-ligands with a general formula M(Htma(N2H42, where, M = Mn, Co, Ni, Cu and Zn; H3tma = trimesic acid, have been prepared and characterized by elemental, structural, spectral and thermal analyses. For the complexes, the carboxylate νasym and νsym stretchings are observed at about 1626 and 1367 cm−1 respectively, with Δν between them of ∼260 cm−1, showing the unidentate coordination of each carboxylate group. The hydrazine moieties are present as bridging bidentates. Electronic and EPR spectral studies suggest an octahedral geometry for the complexes. All these complexes show three steps of decomposition in TGA/DTA. SEM images of CuO and MnO residues obtained from the complexes show nano-sized clusters suggesting that the complexes may be used as precursors for nano-CuO and nano-MnO preparation. The antimicrobial activities of the prepared complexes, against four bacteria have been evaluated.

  11. Novel aminonaphthoquinone mannich bases derived from lawsone and their copper(II) complexes: synthesis, characterization and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Neves, Amanda P.; Barbosa, Claudia C.; Greco, Sandro J.; Vargas, Maria D. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Inst. de Quimica; Visentin, Lorenzo C. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Quimica; Pinheiro, Carlos B. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Fisica; Mangrich, Antonio S. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Centro Politecnico. Dept. de Quimica; Barbosa, Jussara P.; Costa, Gisela L. da [Instituto Oswaldo Cruz, Rio de Janeiro, RJ (Brazil)

    2009-07-01

    A series of novel Mannich bases (HL1-HL13) derived from 2-hydroxy-1,4-naphthoquinone (lawsone), substituted benzaldehydes [C{sub 6}H{sub 2}R{sup 1}R{sup 2}R{sup 3}C(O)H] and various primary amines (NH{sub 2}R{sup 4}, R{sup 4} = n-butyl, benzyl, allyl, 2-furfuryl), and their Cu{sup 2+} complexes, [Cu(L1){sub 2}]-[Cu(L13){sub 2}], have been synthesized and fully characterized by analytical and spectroscopic methods. The structures of complexes 1 (R{sup 1} R{sup 2} = R{sup 3} = H; R{sup 4} = Bu), 2 (R{sup 1} = R{sup 3} = H; R{sup 2} = NO{sub 2}; R{sup 4}= Bu) and 7 (R{sup 1} OH; R{sup 2} = R{sup 3} = H; R{sup 4}= Bu) were determined by single crystal X-ray diffraction studies. All complexes crystallize in centrosymmetric space groups, with a copper atom in the inversion centre. Two L. coordinate through the naphthalen-2-olate oxygen and secondary amine-N atoms, forming six membered chelate rings around the copper atom in a trans-N{sub 2}O{sub 2} environment; spectroscopic data confirm that the other complexes exhibit similar molecular arrangement. The antimicrobial activity of all compounds has been tested on seven different strains of bacteria: Bacillus cereus, Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. In general, Mannich bases were more active than complexes, HL11 (R{sup 1} = OH; R{sup 2} =H; R{sup 3} = Me; R{sup 4}= Bn) and HL13 (R{sup 1} = OH; R{sup 2} = H; R{sup 3} = Br; R{sup 4}= Bn) being the most potent inhibitors. The MIC for the most active compound HL11 against S. Coli was 20 {mu}mol L{sup -1} (8 {mu}g mL{sup -1}), better than Chloramphenicol (90 {mu}mol L{sup -1}) and well below most values reported for other naphthoquinones. (author)

  12. Synthesis and antibacterial evaluation of novel analogs of fluoroquinolones annulated with 6-substituted-2-aminobenzothiazoles

    Directory of Open Access Journals (Sweden)

    Prabodh Chander Sharma

    2015-09-01

    Full Text Available Keeping in view the immense biological importance of fluoroquinolones and aminobenzothiazoles as antimicrobials and in search for better antibacterial agents, design and synthesis of new fluoroquinolone derivatives having substituted piperazine rings at the C-7 position are described in the present communication. The synthesized compounds were characterized by suitable spectroscopic methods. Most of the new compounds (4a–l demonstrated high in vitro antibacterial activity with some compounds exhibiting more potent activities against Gram-positive organisms than those of ciprofloxacin, norfloxacin and gatifloxacin. The results of the present study reveal that the compounds have significant antibacterial potential and are suitable candidates for further exploration.

  13. Synthesis, Characterization and Antibacterial Activity of Novel 1,3-Diethyl-1,3-bis(4-nitrophenylurea and Its Metal(II Complexes

    Directory of Open Access Journals (Sweden)

    Hoda Pasdar

    2017-12-01

    Full Text Available A bioactive ligand and its dinuclear metal(II complexes were synthesized and characterized by Fourier-transform infrared spectroscopy (FT-IR, ultraviolet-visible (UV-Visible, nuclear magnetic resonance (1H-NMR, mass spectroscopy and molar conductance measurements. The ligand has been crystalized in the monoclinic system with a P21/c space group. The biological activities of metal complexes were evaluated using disc diffusion and broth dilution methods. In vitro antibacterial activities of the ligand and their metal complexes were examined against two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus and two Gram-negative bacteria (Escherichia coli and Serratia marcescens and compared to the standard drugs. It was found that metal complexes displayed much higher antibacterial activities and better inhibitory effects than that of the ligand and standard drugs. Among these complexes, the compound having Zn-metal showed greater antibacterial activity against all four tested bacteria and was more effective against Serratia marcescens with the zone inhibition diameter of 26 mm and MIC value of 31.25 µg/mL.

  14. The synthesis and characterization of poly({gamma}-glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Stephen Inbaraj, B; Kao, T H; Tsai, T Y; Chiu, C P; Kumar, R; Chen, B H, E-mail: 002622@mail.fju.edu.tw [Department of Food Science, Fu Jen University, Taipei 242, Taiwan (China)

    2011-02-18

    Magnetite nanoparticles (MNPs) modified with sodium and calcium salts of poly({gamma}-glutamic acid) (NaPGA and CaPGA) were synthesized by the coprecipitation method, followed by characterization and evaluation of their antibacterial and cytotoxic effects. Superparamagnetic MNPs are particularly attractive for magnetic driving as well as bacterial biofilm and cell targeting in in vivo applications. Characterization of synthesized MNPs by the Fourier transform infrared spectra and magnetization curves confirmed the PGA coating on MNPs. The mean diameter of NaPGA- and CaPGA-coated MNPs as determined by transmission electron microscopy was 11.8 and 14 nm, respectively, while the x-ray diffraction pattern revealed the as-synthesized MNPs to be pure magnetite. Based on agar dilution assay, both NaPGA- and CaPGA-coated MNPs showed a lower minimum inhibitory concentration in Salmonella enteritidis SE 01 than the commercial antibiotics linezolid and cefaclor, but the former was effective against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 10832, whereas the latter was effective against Escherichia coli O157:H7 TWC 01. An in vitro cytotoxicity study in human skin fibroblast cells as measured by MTT assay implied the as-synthesized MNPs to be nontoxic. This outcome demonstrated that both {gamma}-PGA-modified MNPs are cytocompatible and possess antibacterial activity in vitro, and thereby should be useful in in vivo studies for biomedical applications.

  15. The synthesis and characterization of poly(γ-glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity

    Science.gov (United States)

    Inbaraj, B. Stephen; Kao, T. H.; Tsai, T. Y.; Chiu, C. P.; Kumar, R.; Chen, B. H.

    2011-02-01

    Magnetite nanoparticles (MNPs) modified with sodium and calcium salts of poly(γ-glutamic acid) (NaPGA and CaPGA) were synthesized by the coprecipitation method, followed by characterization and evaluation of their antibacterial and cytotoxic effects. Superparamagnetic MNPs are particularly attractive for magnetic driving as well as bacterial biofilm and cell targeting in in vivo applications. Characterization of synthesized MNPs by the Fourier transform infrared spectra and magnetization curves confirmed the PGA coating on MNPs. The mean diameter of NaPGA- and CaPGA-coated MNPs as determined by transmission electron microscopy was 11.8 and 14 nm, respectively, while the x-ray diffraction pattern revealed the as-synthesized MNPs to be pure magnetite. Based on agar dilution assay, both NaPGA- and CaPGA-coated MNPs showed a lower minimum inhibitory concentration in Salmonella enteritidis SE 01 than the commercial antibiotics linezolid and cefaclor, but the former was effective against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 10832, whereas the latter was effective against Escherichia coli O157:H7 TWC 01. An in vitro cytotoxicity study in human skin fibroblast cells as measured by MTT assay implied the as-synthesized MNPs to be nontoxic. This outcome demonstrated that both γ-PGA-modified MNPs are cytocompatible and possess antibacterial activity in vitro, and thereby should be useful in in vivo studies for biomedical applications.

  16. Synthesis, characterization and antibacterial investigation of divalent metal complexes of NO-donor ligand: {5-chloro-2-[(4-chlorobenzylidene-amino]phenyl}(phenylmethanone

    Directory of Open Access Journals (Sweden)

    Muhammad Aslam

    2015-05-01

    Full Text Available A ligand L has been synthesized by the condensation of 4-chlorobenzaldehyde and 2-amino-5-chlorobenzophenone. The complexes 1–5 of Pb+2, Ni+2, Co+2, Cu+2 and Cd+2 with ligand were prepared. The newly synthesized ligand L and its complexes 1–5 were characterized on the basis of elemental analyses, molar conductance, 1H NMR, IR and MS spectral data. The ligand was also characterized by X-ray crystallography. By electronic spectra and magnetic moment data, the square planar geometry for complex 1 and the octahedral geometry for complexes 2–5 were proposed. The conductivity data suggests non-electrolytic nature of the complexes 1–5. The complexes have higher antibacterial activity than the parent ligand L.

  17. Synthesis and antibacterial activity of some new heterocycles incorporating phthalazine.

    Science.gov (United States)

    Khalil, A M; Berghot, M A; Gouda, M A

    2009-11-01

    3-(1,4-Dioxo-3,4,4e,5,10,10a-hexahydro-1H-5,10-benzeno-benzo[g]phthalazin-2-yl)-3-oxo-propiononitrile (1) was utilized as key intermediate for the synthesis of some new iminocoumarin 2, chromenone 3, aminothiazole 4, triazepine 5a, b and 6, hydrazono-propiononitrile 7, pyridopyrazotriazine 8, monobromo 9, dibromo 10 quinoxaline 11, ketene N,S-acetal 13, ketene S,S-diacetal 17 and 18a, b and methyl dithioate 20 derivatives, respectively. The newly synthesized compounds were characterized by IR, (1)H NMR, (13)C NMR and mass spectral studies. Representative compounds of the synthesized product were tested and evaluated as antibacterial agent.

  18. Synthesis and Characterization of Some New Cu(II, Ni(II and Zn(II Complexes with Salicylidene Thiosemicarbazones: Antibacterial, Antifungal and in Vitro Antileukemia Activity

    Directory of Open Access Journals (Sweden)

    Tudor Rosu

    2013-07-01

    Full Text Available Thirty two new Cu(II, Ni(II and Zn(II complexes (1–32 with salicylidene thiosemicarbazones (H2L1–H2L10 were synthesized. Salicylidene thiosemicarbazones, of general formula (XN-NH-C(S-NH(Y, were prepared through the condensation reaction of 2-hydroxybenzaldehyde and its derivatives (X with thiosemicarbazide or 4-phenylthiosemicarbazide (Y = H, C6H5. The characterization of the new formed compounds was done by 1H-NMR, 13C-NMR, IR spectroscopy, elemental analysis, magnetochemical, thermoanalytical and molar conductance measurements. In addition, the structure of the complex 5 has been determined by X-ray diffraction method. All ligands and metal complexes were tested as inhibitors of human leukemia (HL-60 cells growth and antibacterial and antifungal activities.

  19. New Eco-Friendly 1-Alkyl-3-(4-phenoxybutyl Imidazolium-Based Ionic Liquids Derivatives: A Green Ultrasound-Assisted Synthesis, Characterization, Antibacterial Activity and POM Analyses

    Directory of Open Access Journals (Sweden)

    Mouslim Messali

    2014-08-01

    Full Text Available In view of the emerging importance of the ILs as “green” materials with wide applications and our general interests in green processes, a series of a twenty five new 1-alkyl-3-(4-phenoxybutyl imidazolium-based ionic liquids (ILs derivatives is synthesized using a facile and green ultrasound-assisted procedure. Their structures were characterized by FT-IR, 1H-NMR, 13C-NMR, 11B, 19F, 31P, and mass spectrometry. Antimicrobial screens of some selected ILs were conducted against a panel of Gram-positive and Gram-negative bacteria. The antimicrobial activity of each compound was measured by determination of the minimal inhibitory concentration (MIC yielding very interesting and promising results. Their antibacterial activities are reported, and, on the basis of the experimental and virtual POM screening data available, attempt is also made to elucidate the structure activity relationship.

  20. A Green Approach for the Synthesis of Silver Nanoparticles Using Ultrasonic Radiation’s Times in Sodium Alginate Media: Characterization and Antibacterial Evaluation

    Directory of Open Access Journals (Sweden)

    Miftah Faried

    2016-01-01

    Full Text Available The synthesis of silver nanoparticles (Ag-NPs was achieved by a simple green chemistry procedure using sodium alginate (Na-Alg under ultrasonic radiation as a stabilizer and physical reducing agent. The effect of radiation time on the synthesis of Ag-NPs was carried out at room temperature until 720 min. The successful formation of Ag-NPs has been confirmed by UV-Vis, XRD, TEM, FESEM-EDX, zeta potential, and FT-IR analyses. The surface plasmon resonance band appeared at the range of 452–465 nm that is an evidence of formation of Ag-NPs. The XRD study showed that the particles are crystalline structure in nature, with a face-centered cubic (fcc structure. The TEM study showed the Ag-NPs have average diameters of around 20.16–22.38 nm with spherical shape. The FESEM-EDX analysis confirmed the spherical shape of Ag-NPs on the surface of Alg and the element of Ag with the high purity. The zeta potential showed high stability of Alg/Ag-NPs especially after 720 min irradiation with value of −67.56 mV. The FT-IR spectrum confirmed that the Ag-NPs have been capped by the Alg with van der Waals interaction. The Alg/Ag-NPs showed the antibacterial activity against Gram-positive and Gram-negative bacteria. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.

  1. Synthesis, spectroscopic and thermal characterization of sulpiride complexes of iron, manganese, copper, cobalt, nickel, and zinc salts. Antibacterial and antifungal activity

    Science.gov (United States)

    Mohamed, Gehad G.; Soliman, Madiha H.

    2010-08-01

    Sulpiride (SPR; L) is a substituted benzamide antipsychotic which is reported to be a selective antagonist of central dopamine receptors and claimed to have mood-elevating properties. The ligation behaviour of SPR drug is studied in order to give an idea about its potentiality towards some transition metals in vitro systems. Metal complexes of SPR have been synthesized by reaction with different metal chlorides. The metal complexes of SPR with the formula [MCl 2(L) 2(H 2O) 2]· nH 2O [M = Mn(II), Co(II), Ni(II), Cu(II) and Zn(II); n = 0-2] and [FeCl 2(HL)(H 2O) 3]Cl·H 2O have been synthesized and characterized using elemental analysis (CHN), electronic (infrared, solid reflectance and 1H NMR spectra) and thermal analyses (TG and DTA). The molar conductance data reveal that the bivalent metal chelates are non-electrolytes while Fe(III) complex is 1:1 electrolyte. IR spectra show that SPR is coordinated to the metal ions in a neutral monodentate manner with the amide O. From the magnetic and solid reflectance spectra, octahedral geometry is suggested. The thermal decomposition processes of these complexes were discussed. The correlation coefficient, the activation energies, E*, the pre-exponential factor, A, and the entropies, Δ S*, enthalpies, Δ H*, Gibbs free energies, Δ G*, of the thermal decomposition reactions have been derived from thermogravimetric (TG) and differential thermogravimetric (DTG) curves. The synthesized ligand and its metal complexes were also screened for their antibacterial and antifungal activity against bacterial species ( Escherichia coli and Staphylococcus aureus) and fungi ( Aspergillus flavus and Candida albicans). The activity data show that the metal complexes are found to have antibacterial and antifungal activity than the parent drug and less than the standard.

  2. Synthesis and Characterization of a Schiff Base Cobalt (III) Complex ...

    African Journals Online (AJOL)

    2017-12-18

    Dec 18, 2017 ... Synthesis and Characterization of a Schiff Base Cobalt (III) Complex and ... zinc, palladium, magnesium and gold and most ..... Synthesis, spectral characterization, solution equilibria, in vitro antibacterial and cytotoxic activities of Cu(II), Ni(II), Mn(II), Co(II) and Zn(II) complexes with Schiff base derived from 5 ...

  3. Design, Synthesis, and Antibacterial Activities of Novel Heterocyclic Arylsulphonamide Derivatives.

    Science.gov (United States)

    Singh, Anuradha; Srivastava, Ritika; Singh, Ramendra K

    2017-02-13

    Design, synthesis, and antibacterial activities of a series of arylsulphonamide derivatives as probable peptide deformylase (PDF) inhibitors have been discussed. Compounds have been designed following Lipinski's rule and after docking into the active site of PDF protein (PDB code: 1G2A) synthesized later on. Furthermore, to assess their antibacterial activity, screening of the compound was done in vitro conditions against Gram-positive and Gram-negative bacterial strains. In silico, studies revealed these compounds as potential antibacterial agents and this fact was also supported by their prominent scoring functions. Antibacterial results indicated that these molecules possessed a significant activity against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli with MIC values ranging from 0.06 to 0.29 μM. TOPKAT results showed that high LD 50 values and the compounds were assumed non-carcinogenic when various animal models were studied computationally.

  4. Synthesis and antibacterial activity of novel enolphosphate derivatives.

    Science.gov (United States)

    Grison, Claude; Barthes, Nicolas; Finance, Chantal; Duval, Raphael E

    2010-10-01

    A new class of enolphosphates derivatives, the 1-alkenyldiphosphates, was designed and a rapid and efficient synthesis for these compounds was developed. These new molecules showed interesting in vitro antibacterial activities (MIC) against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative pathogens including Pseudomonas aeruginosa and Escherichia coli. 2010 Elsevier Inc. All rights reserved.

  5. Synthesis and antibacterial activity of sulfonamide derivatives at C-8 ...

    Indian Academy of Sciences (India)

    Synthesis and antibacterial activity of some novel biologically active sulfonamide derivatives at C-8 alkyl chain of anacardic acid (7a-7l), prepared from commercially available anacardic acid mixture (1a-d). These compounds were tested for Gram positive and Gram negative bacterial cultures; most of the compounds ...

  6. Synthesis and antibacterial activity of 2-amino chromenes arising cyanoiminocoumarins and β-naphthol

    Directory of Open Access Journals (Sweden)

    Lamia Dammak

    2017-01-01

    Full Text Available The synthesis of 2-amino chromene, reported in our previous paper, has been accomplished by the reaction of cyanoiminocoumarin and β-naphthol . The obtained compound was reacted with various electrophilic or nucleophilic reagents. All the new homologous 2-amino-4H-chromenes have been characterized on the basis of their spectral (IR, 1H and 13C NMR data and microanalysis. Four compounds were evaluated in vitro for their preliminary antibacterial activities against five different pathogenic bacterial strains such as Bacillus thuringiensis , Escherichia coli , Staphylococcus aureus, klebsiella pneumonia and Salmonella Sp . Antibacterial activity of each compound was compared with standard drug, Penicillin .

  7. Synthesis, Characterization, Crystal Structure and Antibacterial Activities of Transition Metal(II Complexes of the Schiff Base 2-[(4-Methylphenyliminomethyl]-6-methoxyphenol

    Directory of Open Access Journals (Sweden)

    Guo-Liang Zhao

    2009-05-01

    Full Text Available Five transition metal(II complexes, [ML2Cl2] 1~5, were synthesized from the reaction of MCl2·nH2O (M = Mn, Co, Ni, Cu, Cd and the Schiff base ligand 2-[(4-methylphenyliminomethyl]-6-methoxyphenol (C15H15NO2, L, obtained by condensation of o-vanillin (2-hydroxy-3-methoxybenzaldehyde with p-toluidine. They were characterized by elemental analysis, molar conductance, FT-IR spectra, thermal analysis. The structure of complex 1 was determined by single-crystal X-ray diffraction. Its crystal structure is of monoclinic system, space group P21/c with a = 9.0111(18 Å, b = 11.222(2 Å, c =28.130 (6 Å, α = 90 º, β = 92.29(3 º, γ = 90 º, V = 2867.6(10 Å3, Z = 4. The Mn atom is six-coordinate and displays distorted octahedral geometry.The Schiff base ligand and its complexes have been tested in vitro to evaluate their antibacterial activity against bacteria, viz., Escherichia coli, Staphylococcus aureus and Bacillus subtilis. It has been found that the complexes have higher activity than the corresponding free Schiff base ligand against the same bacteria.

  8. Synthesis, Characterization and Antibacterial Studies of N-(Benzothiazol-2-yl)-4-chlorobenzenesulphonamide and Its Neodymium(III) and Thallium(III) Complexes.

    Science.gov (United States)

    Obasi, Lawrence Nnamdi; Oruma, Uchechukwu Susan; Al-Swaidan, Ibrahim Abdulrazak; Ramasami, Ponnadurai; Ezeorah, Chigozie Julius; Ochonogor, Alfred Ezinna

    2017-02-22

    N -(Benzothiazol-2-yl)-4-chlorobenzenesulphonamide (NBTCS) was synthesized by condensation reaction of 4-chlorobenzenesulphonyl chloride and 2-aminobenzothiazole in acetone under reflux. Neodymium(III) and thallium(III) complexes of the ligand were also synthesized. Both ligand and metal complexes were characterized using UV-Vis, IR, ¹H- and 13 C-NMR spectroscopies, elemental analysis and molar conductance measurement. IR studies revealed that the ligand is tridentate and coordinates to the metal ions through nitrogen and oxygen atoms of the sulphonamide group and nitrogen atom attached to benzothiazole ring. The neodymium(III) complex displays a coordination number of eight while thallium(III) complex displays a coordination number of six. The ligand and its complexes were screened in vitro for their antibacterial activities against Escherichia coli strains ( E. coli 6 and E. coli 13 ), Proteus species, Staphylococcus aureus and Pseudomonas aeruginosa using the agar well diffusion technique. The synthesized compounds were found to be more active against the microorganisms screened relative to ciprofloxacin, gentamicin and co-trimoxazole.

  9. 3,4-Dimethyl diphenyldithiophosphate of mononuclear cobalt(II) with N-donor ligands: Synthesis, structural characterization, DFT and antibacterial studies

    Science.gov (United States)

    Kumar, Sandeep; Kour, Gurpreet; Schreckenbach, Georg; Andotra, Savit; Hundal, Geeta; Sharma, Vishal; Jaglan, Sundeep; Pandey, Sushil K.

    2017-08-01

    3,4-Dimethyl diphenyldithiophosphate of cobalt(II) with N-donor ligands [{(ArO)2PS2}2CoL2] [Ar = 3,4-(CH3)2C6H3 (1-3); L = C5H5N (1), 3,4-(CH3)2C5H3N (2) and 4-(C2H5)C5H4N (3)] have been synthesized and characterized by elemental analyses, infrared spectroscopy (IR), powder X-ray diffraction (PXRD) and single crystal X-ray analysis. Complex 1 crystallizes in the monoclinic space group P21/n whereas complexes 2 and 3 crystallize in the triclinic space group Pbar1. The crystal structures of complexes 1-3 reveal mononuclear units with the Co(II) center chelated in bidentate fashion by four S atoms of the two diphenyldithiophosphate ligands. The N atoms from two donor ligands are axially coordinated, leading to distorted octahedral geometry around Co(II). The complexes have been optimized using density functional theory (DFT), structural parameters have been calculated, and the energy gaps of the frontier orbitals (HOMO-LUMO) have been predicted. Mayer bond orders have also been calculated. Structural parameters from the crystallographic and DFT studies are in good agreement with each other. To explore the biological potential, complexes were evaluated for their antibacterial activity against three bacterial strains. The bacterial growth inhibition capacity of the ligand and complexes followed the order of 3 > 2 > 1 > L1.

  10. DNA-binding, catalytic oxidation, C—C coupling reactions and antibacterial activities of binuclear Ru(II thiosemicarbazone complexes: Synthesis and spectral characterization

    Directory of Open Access Journals (Sweden)

    Arumugam Manimaran

    2012-07-01

    Full Text Available New hexa-coordinated binuclear Ru(II thiosemicarbazone complexes of the type {[(B(EPh3(COClRu]2L} (where, E = P or As; B = PPh3 or AsPh3 or pyridine; L = mononucleating NS donor of N-substituted thiosemicarbazones have been synthesized and characterized by elemental analysis, FT-IR, UV–vis and 31P{1H} NMR cyclic voltammetric studies. The DNA-binding studies of Ru(II complexes with calf thymus DNA (CT-DNA were investigated by UV–vis, viscosity measurements, gel-electrophoresis and fluorescence spectroscopy. The new complexes have been used as catalysts in C—C coupling reaction and in the oxidation of alcohols to their corresponding carbonyl compounds by using NMO as co-oxidant and molecular oxygen (O2 atmosphere at ambient temperature. Further, the new binucleating thiosemicarbazone ligands and their Ru(II complexes were also screened for their antibacterial activity against Klebsiella pneumoniae, Shigella sp., Micrococcus luteus, Escherichia coli and Salmonella typhi. From this study, it was found out that the activity of the complexes almost reaches the effectiveness of the conventional bacteriocide.

  11. Mesoporous silica nanoparticles supported copper(II) and nickel(II) Schiff base complexes: Synthesis, characterization, antibacterial activity and enzyme immobilization

    Science.gov (United States)

    Tahmasbi, Leila; Sedaghat, Tahereh; Motamedi, Hossein; Kooti, Mohammad

    2018-02-01

    Mesoporous silica nanoparticles (MSNs) were prepared by sol-gel method and functionalized with 3-aminopropyltriethoxysilane. Schiff base grafted mesoporous silica nanoparticle was synthesized by the condensation of 2-hydroxy-3-methoxybenzaldehyde and amine-functionalized MSNs. The latter material was then treated with Cu(II) and Ni(II) salts separately to obtain copper and nickel complexes anchored mesoporous composites. The newly prepared hybrid organic-inorganic nanocomposites have been characterized by several techniques such as FT-IR, LA-XRD, FE-SEM, TEM, EDS, BET and TGA. The results showed all samples have MCM-41 type ordered mesoporous structure and functionalization occurs mainly inside the mesopore channel. The presence of all elements in synthesized nanocomposites and the coordination of Schiff base via imine nitrogen and phenolate oxygen were confirmed. MSNs and all functionalized MSNs have uniform spherical nanoparticles with a mean diameter less than 100 nm. The as-synthesized mesoporous nanocomposites were investigated for antibacterial activity against Gram-positive (B. subtilis and S. aureus) and Gram-negative (E. coli and P. aeruginosa) bacteria, as carrier for gentamicin and also for immobilization of DNase, coagulase and amylase enzymes. MSN-SB-Ni indicated bacteriocidal effect against S.aureus and all compounds were found to be good carrier for gentamicin. Results of enzyme immobilization for DNase and coagulase and α-amylase revealed that supported metal complexes efficiently immobilized enzymes.

  12. Synthesis, characterization, redox behavior, DNA and protein binding and antibacterial activity studies of ruthenium(II) complexes of bidentate schiff bases.

    Science.gov (United States)

    Paul, Hena; Sen, Buddhadeb; Mondal, Tapan Kumar; Chattopadhyay, Pabitra

    2017-08-03

    Two new ruthenium(II) complexes of Schiff base ligands (L) derived from cinnamaldehyde and ethylenediamine formulated as [Ru(L)(bpy) 2 ](ClO 4 ) 2 , where L 1 = N,N'-bis(4-nitrocinnamald-ehyde)ethylenediamine and L 2 = N,N'-bis(2-nitrocinnamaldehyde)-ethylenediamine for complex 1 and 2, respectively, were isolated in pure form. The complexes were characterized by physicochemical and spectroscopic methods. The electrochemical behavior of the complexes showed the Ru(III)/Ru(II) couple at different potentials with quasi-reversible voltammograms. The interaction of the complexes with calf thymus DNA (CT-DNA) using absorption, emission spectral studies and electrochemical techniques have been used to determine the binding constant, K b and the linear Stern-Volmer quenching constant, K SV . The results indicate that the ruthenium(II) complexes interact with CT-DNA strongly in a groove binding mode. The interactions of bovine serum albumin (BSA) with the complexes were also investigated with the help of absorption and fluorescence spectroscopy tools. Absorption spectroscopy proved the formation of a ground state BSA-[Ru(L)(bpy) 2 ](ClO 4 ) 2 complex. The antibacterial study showed that the Ru(II) complexes (1 and 2) have better activity than the standard antibiotics but weak activity than the ligands.

  13. Pd(II and Zn(II Based Complexes with Schiff Base Ligands: Synthesis, Characterization, Luminescence, and Antibacterial and Catalytic Activities

    Directory of Open Access Journals (Sweden)

    Zhi-Qiang Feng

    2013-01-01

    Full Text Available Two new metal complexes involving Schiff base ligands, namely, [Pd(L12] (1 and [Zn(L22] (2, [HL1: 2,4-dibromo-6-((E-(mesityliminomethylphenol and HL2: 2-((E-(2,6-diisopropylphenyliminomethyl-4,6-dibromophenol], have been solvothermally synthesized and characterized by elemental analysis, IR-spectroscopy, thermogravimetric analysis, powder X-ray diffraction, and single-crystal X-ray diffraction. Both 1 and 2 are mononuclear cyclometalated complexes with square planar and tetrahedral coordination geometry, respectively. 1 and 2 display photoluminescence in the solid state at 298 K (fluorescence lifetimes τ = 5.521 μs at 508 nm for 1; τ = 3.697 μs at 506 nm for 2. These Schiff base ligands and their metal complexes have been screened for antibacterial activity against several bacteria strains, and the results are compared with the activity of penicillin. Moreover, the Suzuki reaction of 4-bromoanisole with phenylboronic acid by 1 has also been studied.

  14. Synthesis, Characterization and Antibacterial Studies of N-(Benzothiazol-2-yl-4-chlorobenzenesulphonamide and Its Neodymium(III and Thallium(III Complexes

    Directory of Open Access Journals (Sweden)

    Lawrence Nnamdi Obasi

    2017-02-01

    Full Text Available N-(Benzothiazol-2-yl-4-chlorobenzenesulphonamide (NBTCS was synthesized by condensation reaction of 4-chlorobenzenesulphonyl chloride and 2-aminobenzothiazole in acetone under reflux. Neodymium(III and thallium(III complexes of the ligand were also synthesized. Both ligand and metal complexes were characterized using UV-Vis, IR, 1H- and 13C-NMR spectroscopies, elemental analysis and molar conductance measurement. IR studies revealed that the ligand is tridentate and coordinates to the metal ions through nitrogen and oxygen atoms of the sulphonamide group and nitrogen atom attached to benzothiazole ring. The neodymium(III complex displays a coordination number of eight while thallium(III complex displays a coordination number of six. The ligand and its complexes were screened in vitro for their antibacterial activities against Escherichia coli strains (E. coli 6 and E. coli 13, Proteus species, Staphylococcus aureus and Pseudomonas aeruginosa using the agar well diffusion technique. The synthesized compounds were found to be more active against the microorganisms screened relative to ciprofloxacin, gentamicin and co-trimoxazole.

  15. Synthesis and antibacterial activity of new chiral ...

    Indian Academy of Sciences (India)

    The compounds were evaluated for their in vitro antibacterial activity against some Gram-positive bacteria; Staphylococcus aureus and Gram-negative bacteria; Escherichia Coli, Klebsiella pneumonieae, Acinetobacter, Pseudomonas aeruginosa, Enterococcus, Salmonella sp. The compounds showed moderate to good ...

  16. Microwave-assisted synthesis, structural characterization, DFT studies, antibacterial and antioxidant activity of 2-methyl-4-oxo-1,2,3,4-tetrahydroquinazoline-2-carboxylic acid

    Science.gov (United States)

    Obafemi, Craig A.; Fadare, Olatomide A.; Jasinski, Jerry P.; Millikan, Sean P.; Obuotor, Efere M.; Iwalewa, Ezekiel O.; Famuyiwa, Samson O.; Sanusi, Kayode; Yilmaz, Yusuf; Ceylan, Ümit

    2018-03-01

    In the present study a new tetrahydroquinazoline-2-carboxylic, C10H10N2O3, 1‧, was synthesized and its structure was characterized by elemental analysis, IR, 1H NMR, 13C NMR data and high-resolution mass spectrometry. The spectral results are in line with the proposed structure. Single crystal X-ray structural analysis of the compound showed that the crystal structure adopts a monoclinic space group P21/c, with the packing of the molecule stabilized by Cdbnd O … …Hsbnd O, Nsbnd H … ….Odbnd Csbnd Osbnd intermolecular hydrogen bonding. The theoretical geometrical parameters of the compound have been calculated using density functional (DFT) and time-dependent density functional (TD-DFT) theory methods and have been used to predict the thermodynamic one-electron redox potential and the electronic absorption property of the compound. The theoretical characterization matched the experimental measurements, showing a good correlation. The calculated HOMO-LUMO gap (4.79 eV) suggests that compound 1‧ could be a potential antioxidant. The synthesized compound was screened for its in vitro antimicrobial activity against selected bacterial strains and antioxidant activity using the TAC, FRAP, NO and ABTS models. In vitro antioxidant activity of 1' showed a moderate activity, but weaker scavenging activity than the standards of ascorbic acid and trolox. Results of the antibacterial activity of the tested compound showed that it possesses a higher activity against Bacillus anthracis, Bacillus cereus, Bacillus polymyxa, Bacillus subtilis and Staphylococcus aureus than the two standard drugs, streptomycin and tetracycline, and better activity than tetracycline against Escherichia coli.

  17. Synthesis, structural characterization, antibacterial activity, DNA binding and computational studies of bis(2-methyl-1H-imidazole κN3)silver(I)dichromate(VI)

    Science.gov (United States)

    Beheshti, Azizolla; Hashemi, Faezeh; Monavvar, Mohamad Fattahi; Khorrmdin, Rahman; Abrahams, Carmel T.; Motamedi, Hossein; Shakerzadeh, Ehsan

    2017-04-01

    A new silver complex [Ag(C4H6N2)2]2Cr2O7 (1) derived from Ag2[CrO4] and 2-methylimidazole has been synthesized and fully characterized by Single crystal X-ray diffraction, UV-Vis, FT-IR, elemental analysis, DFT and AIM calculations. The crystal structure of 1 shows that the discrete, ionic compound of [Ag(C4H6N2)2]2Cr2O7 consists of two [Ag(C4H6N2)2]+ cations bridged by a [Cr2O7]2- counter anion. In each of the [Ag(C4H6N2)2]+ complexes, the Ag center is coordinated by two N atoms from two distinct neutral monodentate 2-methylimidazole ligands in an almost linear geometry with Agsbnd N distances between 2.068(4) Å and 2.076(4) Å and Nsbnd Agsbnd N bond angles of 179.4(2)° and 173.8(2)°. The Nsbnd H⋯O and Csbnd H ⋯ O hydrogen bonds, π⋯π stacking, Csbnd H⋯π and Ag⋯O interactions play an important role in the structural stabilization of the complex to generate a three-dimensional framework. The antibacterial activity and molecular docking studies of the free 2-methylimidazole ligand and the title complex show that these compounds have ability to inhibit the growth of the tested bacteria. A scanning probe microscopy (SPM) study of the treated bacteria was carried out to investigate the structural changes caused by the interactions between the complex and target bacteria. The interaction of the complex 1 with the E.coli DNA (E-DNA) has been studied by UV-Vis absorption, competitive DNA-binding studies with ethidium bromide by fluorescence and gel electrophoresis techniques. These experiments indicate that the complex interacts with DNA by intercalative binding.

  18. Synthesis and study of some new 1,3-isoindoledione derivatives as potential antibacterial agents.

    Science.gov (United States)

    Khalil, A M; Berghot, M A; Gouda, M A

    2010-04-01

    In an effort to establish new candidates with improved antibacterial activities, we reported here the synthesis and in vitro antibacterial evaluation of various series of 2-substituted-3a,4,9,9a-tetrahydro-4,9-benzeno-benz[f]isoindole-1,3-diones: 4-acetyl-phenyl 2, 2,2-dibromoacetylphenyl 3, benzimidazole 4, acetylbenzimidazole 5, aminophenyl 6, acetamide 7, naphthalene 8, disulfide 9, mercaptophenyl 10, hydroxyphenyl 11, phenyl ester 12, triazole 13, benzothiophene 14, benzothiazole 15 phenylazo 16a, b and aminomethane 17 derivatives. The newly synthesized compounds were characterized by (IR, (1)H NMR, (13)C NMR and mass spectrum studies). Representative compounds of the synthesized products were established and evaluated as antibacterial agents. Copyright (c) 2010 Elsevier Masson SAS. All rights reserved.

  19. Tannic acid-mediated green synthesis of antibacterial silver nanoparticles.

    Science.gov (United States)

    Kim, Tae Yoon; Cha, Song-Hyun; Cho, Seonho; Park, Youmie

    2016-04-01

    The search for novel antibacterial agents is necessary to combat microbial resistance to current antibiotics. Silver nanoparticles (AgNPs) have been reported to be effective antibacterial agents. Tannic acid is a polyphenol compound from plants with antioxidant and antibacterial activities. In this report, AgNPs were prepared from silver ions by tannic acid-mediated green synthesis (TA-AgNPs). The reaction process was facile and involved mixing both silver ions and tannic acid. The absorbance at 423 nm in the UV-Visible spectra demonstrated that tannic acid underwent a reduction reaction to produce TA-AgNPs from silver ions. The synthetic yield of TA-AgNPs was 90.5% based on inductively coupled plasma mass spectrometry analysis. High-resolution transmission electron microscopy and atomic force microscopy images indicated that spherical-shaped TA-AgNPs with a mean particle size of 27.7-46.7 nm were obtained. Powder high-resolution X-ray diffraction analysis indicated that the TA-AgNP structure was face-centered cubic with a zeta potential of -27.56 mV. The hydroxyl functional groups of tannic acid contributed to the synthesis of TA-AgNPs, which was confirmed by Fourier transform infrared spectroscopy. The in vitro antibacterial activity was measured using the minimum inhibitory concentration (MIC) method. The TA-AgNPs were more effective against Gram-negative bacteria than Gram-positive bacteria. The MIC for the TA-AgNPs in all of the tested strains was in a silver concentration range of 6.74-13.48 μg/mL. The tannic acid-mediated synthesis of AgNPs afforded biocompatible nanocomposites for antibacterial applications.

  20. The Evolving Role of Chemical Synthesis in Antibacterial Drug Discovery

    Science.gov (United States)

    Wright, Peter M.; Seiple, Ian B.; Myers, Andrew G.

    2015-01-01

    The discovery and implementation of antibiotics in the early twentieth century transformed human health and wellbeing. Chemical synthesis enabled the development of the first antibacterial substances, organoarsenicals and sulfa drugs, but these were soon outshone by a host of more powerful and vastly more complex antibiotics from nature: penicillin, streptomycin, tetracycline, and erythromycin, among others. These primary defences are now significantly less effective as an unavoidable consequence of rapid evolution of resistance within pathogenic bacteria, made worse by widespread misuse of antibiotics. For decades medicinal chemists replenished the arsenal of antibiotics by semisynthetic and to a lesser degree fully synthetic routes, but economic factors have led to a subsidence of this effort, which places society on the precipice of a disaster. We believe that the strategic application of modern chemical synthesis to antibacterial drug discovery must play a critical role if a crisis of global proportions is to be averted. PMID:24990531

  1. Synthesis, characterization and crystal structures of new Zinc(II) and Nickel(II) complexes containing morpholine moiety and their antibacterial studies

    Czech Academy of Sciences Publication Activity Database

    Goudarziafshar, H.; Rezaeivala, M.; Khosravi, F.; Abbasityula, Y.; Yousefi, S.; Özbek, N.; Eigner, Václav; Dušek, Michal

    2015-01-01

    Roč. 12, č. 1 (2015), 113-119 ISSN 1735-207X Grant - others:AV ČR(CZ) Praemium Academiae Institutional support: RVO:68378271 Keywords : crystal structure * morpholine moiety * antibacterial effects * zinc (ii) complex * nickel(ii) complex Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.300, year: 2015

  2. Green synthesis of silver nanoparticles using a Melissa officinalis leaf extract with antibacterial properties

    Directory of Open Access Journals (Sweden)

    Álvaro de Jesús Ruíz-Baltazar

    Full Text Available The exceptional properties of the silver nanoparticles offer several applications in the biomedicine field. The development of antibiotics which are clinically useful against bacteria and drug resistant microorganisms, it is one of the main approaches of silver nanoparticles. However, it is necessary to develop environmentally friendly methods for their synthesis. In this sense, the main objective of this work is focused on to propose a simplified and efficient green synthesis of silver nanoparticles with proven antibacterial properties. The green synthesis route is based on the use of the Melissa officinalis as reducing agent of the silver ions in aqueous solution at room temperature. Complementary, the antibacterial activity of the silver nanoparticles against Staphylococcus aureus and Escherichia coli was confirmed. The silver nanoparticles obtained were characterized by transmission electron microscopy, X-ray diffraction, UV–vis, Raman and FT-IR spectroscopy. The observed results suggested that using Melissa officinalis, it is possible to performed silver nanoparticles with controlled characteristics and with significant inhibitory activity against the Staphylococcus aureus and Escherichia coli. Keywords: Green synthesis, Nanoparticles, Antibacterial effect

  3. Timber industry waste-teak ( Tectona grandis Linn.) leaf extract mediated synthesis of antibacterial silver nanoparticles

    Science.gov (United States)

    Devadiga, Aishwarya; Shetty, K. Vidya; Saidutta, M. B.

    2015-08-01

    The current research article emphasizes efficacious use of teak leaves, an agro -biowaste from world's premier hardwood timber industry, for "green" synthesis of silver nanoparticles (AgNPs). Bioactive compounds of the leaves act as prolific reducing and stabilizing agents in AgNP synthesis. The characterization of the AgNPs synthesized using teak leaves revealed that the particles are spherical with an average size of 28 nm and the presence of bioactive compounds present in teak leaf extract as capping agents on the nanoparticles. A prominent decrease in the content of bioactive compounds such as polyphenols, antioxidants and flavonoids after the biosynthesis of AgNPs signifies that these class of compounds act as reductants and stabilizers during biosynthesis. The biosynthesized silver nanoparticles were also successfully evaluated for their antibacterial characteristics against waterborne pathogens, E. coli and S. aureus, with minimum inhibitory concentration of 25.6 μg/mL. Exploitation of agrowaste resources for synthesis of AgNPs curtails indiscriminate usage of food and commercial plant materials, rather contributing a sustainable way for effective plant waste biomass utilization and management. The biosynthesized AgNps have potential application in water purifiers, antibacterial fabrics, sports wear and in cosmetics as antibacterial agent and the process used for its synthesis being greener is highly beneficial from environmental, energy consumption and economic perspectives.

  4. Synthesis and Antibacterial Activity of Some 2-Substituted ...

    African Journals Online (AJOL)

    2-Substituted tinidazole analogues were designed, synthesized and characterized. The synthesized compounds were tested for their antibacterial activity against Gram-positive (B. subtilis and S. aureus) and Gram-negative (E. coli (entero pathogen) and S. typhimurium) bacteria using agar well diffusion method.

  5. Synthesis and antibacterial evaluation of some novel mannich ...

    African Journals Online (AJOL)

    Substituted benzimidazoles are known for their chemotherapeutic importance and many pharmacological properties. In this paper, we synthesized some novel Mannich bases of benzimidazole derivatives. The synthesized compounds were characterized by their physical and spectral data and in vitro antibacterial activity of ...

  6. Synthesis and studies of antibacterial activity of pongaglabol

    Indian Academy of Sciences (India)

    Pongaglabol [8-hydroxy-5-phenyl-furo[2,3-h]benzo(b)pyran-7-one] was synthesized and tested for antibacterial effects against Shigella dysenteriae, Salmonella typhi, Streptococcus -haemolyticus and Staphylococcus aureus. The synthesized compounds were characterized using UV, IR and 1H NMR spectral data.

  7. Synthesis and characterization of ZnO and Ag nanoparticle-loaded TiO{sub 2} nanotube composite layers intended for antibacterial coatings

    Energy Technology Data Exchange (ETDEWEB)

    Roguska, A., E-mail: aroguska@ichf.edu.pl [Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw (Poland); Pisarek, M. [Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw (Poland); Andrzejczuk, M.; Lewandowska, M. [Faculty of Material Science and Engineering, Warsaw University and Technology, Wołoska 141, 02-507 Warsaw (Poland)

    2014-02-28

    In this work we have fabricated nanoporous oxide layers on Ti with the addition of Ag or ZnO nanoparticles in order to obtain functional coatings ensuring both biocompatibility and antibacterial properties. The morphological and chemical features of the composite coatings fabricated were studied with the aid of high-resolution scanning electron microscopy and surface analytical techniques such as Auger electron spectroscopy and X-ray photoelectron spectroscopy. Our results have shown that Ag and ZnO nanoparticles can be incorporated in a simple and economic manner, suitable for the fabrication of a bactericidal material. The amount of the nanoparticles is variable and depends on the deposition process conditions. The nanoparticles are distributed homogeneously at the top surface of the coating, however they exhibit in depth distribution gradient. This may be promising for maintaining a steady antibacterial effect. - Highlights: • ZnO and/or Ag nanoparticle-loaded TiO{sub 2} nanotube composite layers were fabricated. • Homogeneous surface distribution of ZnO and Ag nanoparticles was achieved. • ZnO and/or Ag/TiO{sub 2} nanotube coatings may exhibit synergistic antibacterial activity.

  8. Green Synthesis, Spectrofluorometric Characterization and Antibacterial Activity of Heterocyclic Compound from Chalcone on the Basis of in Vitro and Quantum Chemistry Calculation.

    Science.gov (United States)

    Khan, Salman A

    2017-05-01

    2-amino-4-(4-bromophenyl)-8-methoxy-5,6-dihydrobenzo[h]quinoline-3-carbonitrile (ABDC) was synthesized by the reaction of (2E)-2-(4 bromobenzylidene) - 6 -methoxy-3,4-dihydronaphthalen-1(2H)-one (Chalcone) with malononitrile and ammonium acetate under microwave irradiation. Chalcone was synthesised by the reaction 4-bromobenzaldehyd, 6-methoxy-1,2,3,4-tetrahydro-naphthalin-1-one under the same condition. Structure of ABDC was conformed by 1 H and 13 C NMR, FT-IR, EI-MS spectral studies and elemental analysis. The electronic absorption and fluorescence spectra of ABDC have been studied in solvents of different polarities, and the data were used to study the solvatochromic properties such as excitation coefficient, stokes shift, oscillator strength, transition dipole moment and fluorescence quantum yield. The absorption maximum and fluorescence emission maximum was observed red shift when increase solvent polarity n-Hexane to DMSO. ABDC undergoes solubilization in different micelles and may be used as a probe and quencher to determine the critical micelle concentration (CMC) of CTAB and SDS. The anti-bacterial activity of chalcone and its cyclized product ABDC was tested in vitro by the disk diffusion assay against two Gram-positive and two Gram-negative bacteria was determined with the reference of standard drug Tetracycline. Results showed that the ABDC is better anti-bacterial agent as compared to chalcone. The anti-bacterial activity was further supported by the quantum chemistry calculation.

  9. Synthesis and characterization of ZnO:CeO2:nanocellulose:PANI bionanocomposite. A bimodal agent for arsenic adsorption and antibacterial action.

    Science.gov (United States)

    Nath, B K; Chaliha, C; Kalita, E; Kalita, M C

    2016-09-05

    In the present study we report the generation of a bimodal, ZnO:CeO2:nanocellulose:polyaniline bionanocomposite having an appreciable remediation efficiency for dissolved Arsenic along with a noticeable antibacterial activity. The microstructural analysis of the synthesized bionanocomposite was carried out by TEM, XRD and FTIR studies, which confirmed the incorporation of the nanoscaled ZnO and CeO2 in the polymeric nanocellulose:polyaniline matrix. The bionanocomposite exhibited a remediation efficiency above ∼95% against As under different adsorbent concentrations and pH conditions. The biosorption mechanism of As on the nanobiosorbent was found to conform to the Freundlich and Dubinin-Radushkevich isotherms. Antibacterial assays for the bionanocomposite showed a high antibacterial activity with MIC50 values of 10.6μgml(-1) against the Gram-positive Bacillus subtilis and 10.3μgml(-1) against the Gram-negative Escherichia coli. Thus, the bionanocomposite shall be of high interest as a novel and sustainable matrix for the design of coats/devices that effectuate arsenic adsorption and microbial control, to generate contaminant free potable water. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. SYNTHESIS AND ANTIBACTERIAL EVALUATION OF SOME ...

    African Journals Online (AJOL)

    General procedure for the synthesis of Mannich Bases (III-V). Benzimidazolylthioacetic acid. (II) (0.002 mol) dissolved in ethanol and 3-4 drops of conc. HCl was added and reaction mixture was kept for stirring. To the stirring reaction mixture, formaldehyde (0.002 mol) was added drop wise and stirring was continued for 10 ...

  11. Progress in Research on Synthesis and Antibacterial Applications of Silver Nanoparticles

    Directory of Open Access Journals (Sweden)

    YE Wei-jie

    2017-09-01

    Full Text Available Pathogenic microorganism is a serious threat to human health. As a novel kind of antibacterial materials, silver nanoparticles involving their preparation approaches and applications are of great research interest in the field of nanomaterials. This review summarized a summary of synthesis methods of silver nanoparticles, including polysaccharide, Tollens, irradiation, biological and polyoxometalates, which enjoy numerous advantages such as wide range of raw materials, gentle reaction condition, low-cost and environmental-friendly and etc.. Furthermore, based on the antibacterial property of silver nanoparticles, the antibacterial mechanism and applications were described. The development of silver nanoparticles in antibacterial application was also prospected, such as antibacterial coating and antibacterial packaging.

  12. Blue green alga mediated synthesis of gold nanoparticles and its antibacterial efficacy against Gram positive organisms

    Energy Technology Data Exchange (ETDEWEB)

    Uma Suganya, K.S. [Centre for Ocean Research, Sathyabama University, Chennai 600 119 (India); Govindaraju, K., E-mail: govindtu@gmail.com [Centre for Ocean Research, Sathyabama University, Chennai 600 119 (India); Ganesh Kumar, V.; Stalin Dhas, T.; Karthick, V. [Centre for Ocean Research, Sathyabama University, Chennai 600 119 (India); Singaravelu, G. [Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632115 (India); Elanchezhiyan, M. [Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600113 (India)

    2015-02-01

    Biofunctionalized gold nanoparticles (AuNPs) play an important role in design and development of nanomedicine. Synthesis of AuNPs from biogenic materials is environmentally benign and possesses high bacterial inhibition and bactericidal properties. In the present study, blue green alga Spirulina platensis protein mediated synthesis of AuNPs and its antibacterial activity against Gram positive bacteria is discussed. AuNPs were characterized using Ultraviolet–visible (UV–vis) spectroscopy, Fluorescence spectroscopy, Fourier Transform-Infrared (FTIR) spectroscopy, Raman spectroscopy, High Resolution-Transmission Electron Microscopy (HR-TEM) and Energy Dispersive X-ray analysis (EDAX). Stable, well defined AuNPs of smaller and uniform shape with an average size of ∼ 5 nm were obtained. The antibacterial efficacy of protein functionalized AuNPs were tested against Gram positive organisms Bacillus subtilis and Staphylococcus aureus. - Highlights: • Size controlled synthesis of gold nanoparticles from blue green alga Spirulina platensis • Stability of gold nanoparticles at different temperatures • Potent antibacterial efficacy against Gram positive organisms.

  13. Synthesis, Characterization, and Antibacterial Activities of Novel Sulfonamides Derived through Condensation of Amino Group Containing Drugs, Amino Acids, and Their Analogs

    Directory of Open Access Journals (Sweden)

    Muhammad Abdul Qadir

    2015-01-01

    Full Text Available Novel sulfonamides were developed and structures of the new products were confirmed by elemental and spectral analysis (FT-IR, ESI-MS, 1HNMR, and 13CNMR. In vitro, developed compounds were screened for their antibacterial activities against medically important gram (+ and gram (− bacterial strains, namely, S. aureus, B. subtilis, E. coli, and K. pneumoniae. The antibacterial activities have been determined by measuring MIC values (μg/mL and zone of inhibitions (mm. Among the tested compounds, it was found that compounds 5a and 9a have most potent activity against E. coli with zone of inhibition: 31±0.12 mm (MIC: 7.81 μg/mL and 30±0.12 mm (MIC: 7.81 μg/mL, respectively, nearly as active as ciprofloxacin (zone of inhibition: 32±0.12 mm. In contrast, all the compounds were totally inactive against the gram (+ B. subtilis.

  14. Synthesis, characterization of some novel 1,3,4-oxadiazole compounds containing 8-hydroxy quinolone moiety as potential antibacterial and anticancer agents

    Directory of Open Access Journals (Sweden)

    Vinayak Mahadev Adimule

    2014-12-01

    Full Text Available In the present work a series of novel derivatives of 8-hydroxy quinolone substituted 1,3,4-oxadiazole compounds were synthesized by convergent synthetic method and studied for their antibacterial and anticancer properties. The cell lines used for cytotoxic evaluation were HeLa, Caco-2 and MCF7. The synthetic chemistry involved conversion of various substituted aromatic acids into ethyl ester 2a-e. The ethyl ester was converted into corresponding carbohydrazide 3a-e. Carbohydrazides are reacted with chloroacetic acid, phosphorous oxytrichloride and irradiated with microwave in order to obtain the various key intermediates 2-(chloromethyl-5-(substituted phenyl-1,3,4-oxadiazole 4a-e. The 2-(chloromethyl-5-(substituted phenyl-1,3,4-oxadiazole was reacted with 8-hydroxy quinolone in presence of sodium hydride and obtained a series of 8-hydroxy quinoline substituted 1,3,4-oxadiazoles 5a-e. Among the synthesised compounds, the cytotoxicity of the compound 5b i.e. 8-{[5-(2,4-dichlorophenyl-1,3,4-oxadiazol-2-yl]methoxy}quinoline against MCF7 with IC50 of 5.3µM and the compound 5e i.e. 8-{[5-(4-bromophenyl-1,3,4-oxadiazol-2-yl]methoxy}quinoline showed MIC of < 6.25µg/mL against Staphylococcus aureus which is comparable with the known standards. The standards used for cytotoxic evaluation was 5-fluorouracil and for antibacterial was nitrofurazone

  15. Heteroleptic complexes of Zn(II) based on 1-(5-bromo-2-hydroxybenzylidene)-4-phenylthiosemicarbazide: Synthesis, structural characterization, theoretical studies and antibacterial activity

    Science.gov (United States)

    Azarkish, Mohammad; Akbari, Alireza; Sedaghat, Tahereh; Simpson, Jim

    2017-04-01

    Four new ternary complexes, [ZnL (2,2‧-bipy)] (1), Zn2L2(4,4‧-bipy)] (2), [ZnL(Imd)]·H2O (3) and [ZnL3(MeImd)] (4), have been synthesized from the reaction of Zn(II) acetate with 1-(5-bromo-2-hydroxybenzylidene)-4-phenylthiosemicarbazide (H2L) in the presence of a heterocyclic base, 2,2‧-bipyridine, 4,4‧-bipyridine, imidazole or 2-methylimidazole, as an auxiliary ligand. The complexes have been investigated by elemental analysis and FT-IR, UV-Vis and 1HNMR spectroscopy. These data show that the thiosemicarbazone acts as a tridentate dianionic ligand and coordinates via the thiol group, imine nitrogen, and phenolic oxygen. The coordination sphere was completed by the nitrogen atom(s) of the secondary ligand. The structure of 1 was also confirmed by X-ray crystallography and shown to be a five coordinate complex with coordination geometry between the square-pyramidal and trigonal-bipyramidal. Density functional theory (DFT) calculations including geometry optimization, vibrational frequencies and electronic absorptions have been performed for 1 with the B3LYP functional at the TZP(6-311G*) basis set using the Gaussian 03 or ADF 2009 packages. The optimization calculation showed that the crystallographically determined geometry parameters can be reproduced with that basis set. Experimental IR frequencies and calculated vibration frequencies also support each other. The in vitro antibacterial activities of the ligand and complexes have been evaluated against Gram-positive (B. subtilis and S. aureus) and Gram-negative (P. aeruginosa) bacteria and compared with the standard antibacterial drugs. The results reveal that all of the complexes show much better activity in comparison to the individual thiosemoicarbazone ligand (H2L), against all bacterial strains used, with complex 3 showing the most promising results.

  16. Preparation, Characterization and Antibacterial Properties of Silver ...

    African Journals Online (AJOL)

    electron microscopy (SEM), differential scanning calorimetry (DSC), dynamic light scattering (DLS) and laser Doppler electrophoresis (LDE). The antibacterial properties of the nanoparticles were also evaluated by agar diffusion method. Results: The size of the silver-chitosan nanoparticles, ranging from 21.9 to 175.3 nm, ...

  17. Purification, Characterization and Antibacterial Mechanism of ...

    African Journals Online (AJOL)

    The agent is a hydrophobic protein with more acidic groups. Keywords: Lactobacillus acidophilus ... kind of antibacterial polypeptide, protein or protein compound [2-4] produced by the ..... A = E. coli cells were treated by the bacteriocins (magnification of 6,000, scale bar = 10 μm); B = no treatment as control (magnification of ...

  18. A purely green synthesis of silver nanoparticles using Carica papaya, Manihot esculenta, and Morinda citrifolia: synthesis and antibacterial evaluations.

    Science.gov (United States)

    Syafiuddin, Achmad; Salmiati; Hadibarata, Tony; Salim, Mohd Razman; Kueh, Ahmad Beng Hong; Sari, Ajeng Arum

    2017-09-01

    Green procedure for synthesizing silver nanoparticles (AgNPs) is currently considered due to its economy and toxic-free effects. Several existing works on synthesizing AgNPs using leaves extract still involve the use of physical or mechanical treatment such as heating or stirring, which consume a lot of energy. To extend and explore the green extraction philosophy, we report here the synthesis and antibacterial evaluations of a purely green procedure to synthesize AgNPs using Carica papaya, Manihot esculenta, and Morinda citrifolia leaves extract without the aforementioned additional treatment. The produced AgNPs were characterized using the ultraviolet-visible spectroscopy, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and antibacterial investigations. For antibacterial tests, two bacteria namely Escherichia coli and Bacillus cereus were selected. The presently employed method has successfully produced spherical AgNPs having sizes ranging from 9 to 69 nm, with plasmonic characteristics ranging from 356 to 485 nm, and energy-dispersive X-ray peak at approximately 3 keV. In addition, the smallest particles can be produced when Manihot esculenta leaves extract was applied. Moreover, this study also confirmed that both the leaves and synthesized AgNPs exhibit the antibacterial capability, depending on their concentration and the bacteria type.

  19. Study on Synthesis and Antibacterial Properties of Ag NPs/GO Nanocomposites

    Directory of Open Access Journals (Sweden)

    Lei Huang

    2016-01-01

    Full Text Available Using graphene oxide as substrate and stabilizer for the silver nanoparticles, silver nanoparticles-graphene oxide (Ag NPs/GO composites with different Ag loading were synthesized through a facile solution-phase method. During the synthesis process, AgNO3 on GO matrix was directly reduced by NaBH4. The structure characterization was studied through X-ray diffraction (XRD, atomic force microscopy (AFM, high-resolution transmission electron microscope (HRTEM, ultraviolet-visible spectroscopy (UV-Vis, and selected area electron diffraction (SAED. The results show that Ag nanoparticles (Ag NPs with the sizes ranging from 5 to 20 nm are highly dispersed on the surfaces of GO sheets. The shape and size of the Ag NPs are decided by the volume of initial AgNO3 solution added in the GO. The antibacterial activities of Ag NPs/GO nanocomposites were investigated and the result shows that all the produced composites exhibit good antibacterial activities against Gram-negative (G− bacterial strain Escherichia coli (E. coli and Gram-positive (G+ strain Staphylococcus aureus (S. aureus. Moreover, the antibacterial activities of Ag NPs/GO nanocomposites gradually increased with the increasing of volume of initial AgNO3 solution added in the GO and this improvement of the antibacterial activities results from the combined action of size effect and concentration effect of Ag NPs in Ag NPs/GO nanocomposites.

  20. Sustainable synthesis of silver nanoparticles using macroalgae Spirogyra varians and analysis of their antibacterial activity

    Directory of Open Access Journals (Sweden)

    Zeinab Salari

    2016-07-01

    Full Text Available In this research, silver nanoparticles (SNPs were synthesized through bio-reduction of silver ions using the Spirogyra varians. The procedure used is simple and sustainable making it suitable for economic production of SNPs. The structure and morphology of SNPs were characterized by UV–visible spectroscopy, X-ray diffraction (XRD pattern, scanning electron microscopy (SEM and Fourier Transform Infra-Red (FTIR. These nanoparticles indicated an absorption peak at 430 nm in the UV–visible spectrum. The crystallite average size was estimated about 17.6 nm and SEM image confirmed synthesis of relatively uniform nanoparticles. The antibacterial effect of SNPs was also tested on several micro-organisms by measuring the inhibition zone, MIC and MBC. The results confirmed that SNPs can act as a powerful antibacterial agent against various pathogenic bacteria.

  1. A Green Ultrasound Synthesis, Characterization and Antibacterial Evaluation of 1,4-Disubstituted 1,2,3-Triazoles Tethering Bioactive Benzothiazole Nucleus

    OpenAIRE

    Nadjet Rezki

    2016-01-01

    The synthesis of N-(benzo[d]thiazol-2-yl)-2-(4-substituted-1H-1,2,3-triazol-1-yl)acetamides 5a–r via the 1,3-dipolar cycloaddition reaction between 2-azido-N-(benzo[d]thiazol-2-yl)acetamide derivatives 3a–c and different alkynes were performed in the presence and absence of ultrasound irradiation. The synthesis was carried out using t-BuOH/H2O (1:1, v/v) as reaction solvents and CuSO4·5H2O/sodium ascorbate as the catalyst. The copper catalyst was implemented to provide the regioselective 1,4-...

  2. Synthesis and antibacterial activity of silver nanoparticles with different sizes

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-Castanon, G. A., E-mail: mtzcastanon@fciencias.uaslp.m [UASLP, Maestria en Ciencias Odontologicas, Facultad de Estomatologia (Mexico); Nino-Martinez, N. [UASLP, Instituto de Metalurgia (Mexico); Martinez-Gutierrez, F., E-mail: fidel@uaslp.m [UASLP, Facultad de Ciencias Quimicas (Mexico); Martinez-Mendoza, J. R.; Ruiz, Facundo [UASLP, Facultad de Ciencias (Mexico)

    2008-12-15

    Silver nanoparticles with different sizes (7, 29, and 89 nm mean values) were synthesized using gallic acid in an aqueous chemical reduction method. The nanoparticles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), and ultraviolet-visible (UV-Vis) absorption spectroscopy; the antibacterial activity was assessed using the standard microdilution method, determining the minimum inhibitory concentration (MIC) according to the National Committee for Clinical Laboratory Standards. From the microscopies studies (TEM) we observed that silver nanoparticles have spherical (7 and 29 nm) and pseudospherical shape (89 nm) with a narrow size distribution. The sizes of the silver nanoparticles were controlled by varying some experimental conditions. It was found that the antibacterial activity of the nanoparticles varies when their size diminishes.

  3. Synthesis and antibacterial activity of silver nanoparticles with different sizes

    International Nuclear Information System (INIS)

    Martinez-Castanon, G. A.; Nino-Martinez, N.; Martinez-Gutierrez, F.; Martinez-Mendoza, J. R.; Ruiz, Facundo

    2008-01-01

    Silver nanoparticles with different sizes (7, 29, and 89 nm mean values) were synthesized using gallic acid in an aqueous chemical reduction method. The nanoparticles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), and ultraviolet-visible (UV-Vis) absorption spectroscopy; the antibacterial activity was assessed using the standard microdilution method, determining the minimum inhibitory concentration (MIC) according to the National Committee for Clinical Laboratory Standards. From the microscopies studies (TEM) we observed that silver nanoparticles have spherical (7 and 29 nm) and pseudospherical shape (89 nm) with a narrow size distribution. The sizes of the silver nanoparticles were controlled by varying some experimental conditions. It was found that the antibacterial activity of the nanoparticles varies when their size diminishes.

  4. A Green Ultrasound Synthesis, Characterization and Antibacterial Evaluation of 1,4-Disubstituted 1,2,3-Triazoles Tethering Bioactive Benzothiazole Nucleus

    Directory of Open Access Journals (Sweden)

    Nadjet Rezki

    2016-04-01

    Full Text Available The synthesis of N-(benzo[d]thiazol-2-yl-2-(4-substituted-1H-1,2,3-triazol-1-ylacetamides 5a–r via the 1,3-dipolar cycloaddition reaction between 2-azido-N-(benzo[d]thiazol-2-ylacetamide derivatives 3a–c and different alkynes were performed in the presence and absence of ultrasound irradiation. The synthesis was carried out using t-BuOH/H2O (1:1, v/v as reaction solvents and CuSO4·5H2O/sodium ascorbate as the catalyst. The copper catalyst was implemented to provide the regioselective 1,4-disubstituted 1,2,3-triazoles 5a–r. Significant reductions in reaction times with comparably higher yields were observed when the reactions were carried out under ultrasound irradiation. The structures of the newly synthesized 1,2,3-triazoles were elucidated by IR, NMR, MS, and elemental analyses. They were also screened for their antimicrobial activity against three gram-positive (Streptococcus pneumonia, Bacillus subtilis, and Staphylococcus aureus, three gram-negative (Pseudomonas aeuroginosa, Escherichia coli, and Klebsiella pneumonia, and two fungal strains (Aspergillus fumigates and Candida albicans. Most of the tested compounds displayed promising antimicrobial activities at a Minimum Inhibition Concentration (MIC of 4–16 μg/mL.

  5. A Green Ultrasound Synthesis, Characterization and Antibacterial Evaluation of 1,4-Disubstituted 1,2,3-Triazoles Tethering Bioactive Benzothiazole Nucleus.

    Science.gov (United States)

    Rezki, Nadjet

    2016-04-18

    The synthesis of N-(benzo[d]thiazol-2-yl)-2-(4-substituted-1H-1,2,3-triazol-1-yl)acetamides 5a-r via the 1,3-dipolar cycloaddition reaction between 2-azido-N-(benzo[d]thiazol-2-yl)acetamide derivatives 3a-c and different alkynes were performed in the presence and absence of ultrasound irradiation. The synthesis was carried out using t-BuOH/H₂O (1:1, v/v) as reaction solvents and CuSO₄·5H₂O/sodium ascorbate as the catalyst. The copper catalyst was implemented to provide the regioselective 1,4-disubstituted 1,2,3-triazoles 5a-r. Significant reductions in reaction times with comparably higher yields were observed when the reactions were carried out under ultrasound irradiation. The structures of the newly synthesized 1,2,3-triazoles were elucidated by IR, NMR, MS, and elemental analyses. They were also screened for their antimicrobial activity against three gram-positive (Streptococcus pneumonia, Bacillus subtilis, and Staphylococcus aureus), three gram-negative (Pseudomonas aeuroginosa, Escherichia coli, and Klebsiella pneumonia), and two fungal strains (Aspergillus fumigates and Candida albicans). Most of the tested compounds displayed promising antimicrobial activities at a Minimum Inhibition Concentration (MIC) of 4-16 μg/mL.

  6. Characterization and Antibacterial Activity of a Glycoside Antibiotic ...

    African Journals Online (AJOL)

    In the present study, characterization and antibacterial activity of a glycoside antibiotic from fermentation broth of bioactive Streptomyces variabilis PO-178 recovered previously from Western Ghat soil of Agumbe, Karnataka, India was investigated. Mass cultivation of the strain PO-178 was carried out in Starch casein nitrate ...

  7. Characterization of Selective Antibacterial Peptides by Polarity Index

    Directory of Open Access Journals (Sweden)

    C. Polanco

    2012-01-01

    Full Text Available In the recent decades, antibacterial peptides have occupied a strategic position for pharmaceutical drug applications and became subject of intense research activities since they are used to strengthen the immune system of all living organisms by protecting them from pathogenic bacteria. This work proposes a simple and easy statistical/computational method through a peptide polarity index measure by which an antibacterial peptide subgroup can be efficiently identified, that is, characterized by a high toxicity to bacterial membranes but presents a low toxicity to mammal cells. These peptides also have the feature not to adopt to an alpha-helicoidal structure in aqueous solution. The double-blind test carried out to the whole Antimicrobial Peptide Database (November 2011 showed an accuracy of 90% applying the polarity index method for the identification of such antibacterial peptide groups.

  8. Synthesis of novel ferrocenyl Mannich bases and their antibacterial activities

    Science.gov (United States)

    Liu, Yuting; Xin, Hong; Yin, Jingyi; Yin, Dawei; Yang, Zijiang; Li, Jie

    2018-04-01

    In this work, a series of Mannich bases bearing ferrocenyl groups were synthesized via Mannich reactions. The effects of different conditions on the reaction were explored, and the newly synthesized compounds were well characterized by NMR and FT-IR. All compounds have been screened for in vitro antibacterial and antifungal, and the compounds 1-ferrocenyl-3-phenyl-3-(m-nitrophenylamino)-1-acetone (2f) and 1-ferrocenyl-3-phenyl-3-(p-nitrophenylamino)-1-acetone (2g) were shown to be significant activity against all the tested bacterial strains, such as Staphylococcus aureus, Streptococcus, Actinomycete, Escherichia coli, Saccharomyces cerevisiae.

  9. Synthesis and antibacterial activity of of silver nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Maliszewska, I; Sadowski, Z [Department of Chemistry, Technical University of Wroclaw, Wybrzeze Wyspianskiego 27, 50-370 Wrocnw (Poland)], E-mail: irena.helena.maliszewska@pwr.wroc.pl

    2009-01-01

    Silver nanoparticles have been known to have inhibitory and bactericidal effects but the antimicrobial mechanism have not been clearly revealed. Here, we report on the synthesis of metallic nanoparticles of silver using wild strains of Penicillium isolated from environment. Kinetics of the formation of nanosilver was monitored using the UV-Vis. TEM micrographs showed the formation of silver nanoparticles in the range 10-100 nm. Obtained Ag nanoparticles were evaluated for their antimicrobial activity against the gram-positive and gram-negative bacteria. As results, Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were effectively inhibited. Nanosilver is a promising candidate for development of future antibacterial therapies because of its wide spectrum of activity.

  10. Transition metal complexes of 5-bromosalicylidene-4-amino-3-mercapto-1,2,4-triazine-5-one: Synthesis, characterization, catalytic and antibacterial studies

    Directory of Open Access Journals (Sweden)

    AYALOOR SUBRAMANIAN RAMASUBRAMANIAN

    2011-01-01

    Full Text Available Transition metal complexes of 5-bromosalicylidene-4-amino-3-mercapto-1,2,4-triazine-5-one with metal precursors, such as Cu(II, Ni(II, Co(II and Pd(II, were synthesized and characterized by physico–chemical and spectroscopic techniques. All the complexes are of the ML type. Based on analytical, spectral data and magnetic moments, the Co(II and Ni(II complexes were assigned octahedral geometries, while the Cu (II and Pd(II complexes square planar. A study on the catalytic oxidation of benzyl alcohol, cyclohexanol, cinnamyl alcohol, 2-propanol and 2-methyl-1-propanol was performed with N-methylmorpholine-N-oxide (NMO as co-oxidant. All the complexes and their parent organic moiety were screened for their biological activity on several pathogenic bacteria and were found to possess appreciable bactericidal properties.

  11. Synthesis and antibacterial evaluation of anziaic acid and analogues as topoisomerase I inhibitors

    OpenAIRE

    Lin, Hao; Annamalai, Thirunavukkarasu; Bansod, Priyanka; Tse-Dinh, Yuk-Ching; Sun, Dianqing

    2013-01-01

    Naturally occurring anziaic acid was very recently reported as a topoisomerase I inhibitor with antibacterial activity. Herein total synthesis of anziaic acid and structural analogues is described and the preliminary structure-activity relationship (SAR) has been developed based on topoisomerase inhibition and whole cell antibacterial activity.

  12. Indole-2-carboxylic acid derived mono and bis 1,4-disubstituted 1,2,3-triazoles: Synthesis, characterization and evaluation of anticancer, antibacterial, and DNA-cleavage activities.

    Science.gov (United States)

    Narsimha, Sirassu; Satheesh Kumar, Nukala; Kumara Swamy, Battula; Vasudeva Reddy, Nagavelli; Althaf Hussain, S K; Srinivasa Rao, M

    2016-03-15

    A series of new indole-2-carboxylic acid derived mono and bis 1,4-disubstituted 1,2,3 triazoles (I(1)-I(6) and I(7)-I(12)) were synthesized and screened for their anticancer (in vitro and in vivo), antibacterial, and DNA cleavage activities. All the synthesized compounds were characterized by spectral studies. The in vitro anticancer screening results revealed that compound I(12) has registered potential activity against MCF-7, HeLa and HEK293 as compared with the standard reference drug Cisplatin. Remaining compounds have exhibited moderate to good activity against three cancer cell lines. The antibacterial activity screening results revealed that compounds, I(6) and I(12) have registered excellent inhibition against Escherichia coli and Bacillus subtilis in comparison with the standard drug Streptomycin. Compounds I(2) and I(11) have partially cleaved the DNA at 100 μg mL(-1) concentration. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Antibacterial Activity of Green Synthesis of Iron Nanoparticles Using Lawsonia inermis and Gardenia jasminoides Leaves Extract

    Directory of Open Access Journals (Sweden)

    Tayyaba Naseem

    2015-01-01

    Full Text Available Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metallic nanoparticles has accumulated an ultimate interest over the last decade due to their distinctive properties that make them applicable in various fields of science and technology. Metal nanoparticles that are synthesized by using plants have emerged as nontoxic and ecofriendly. In this study a very cheap and simple conventional heating method was used to obtain the iron nanoparticles (FeNPs using the leaves extract of Lawsonia inermis and Gardenia jasminoides plant. The iron nanoparticles were characterized by thermal gravimetric analysis (TGA, Fourier transform infrared spectroscopy (FT-IR, transmission electron microscopy (TEM, scanning electron microscopy (SEM, atomic force microscopy (AFM, and X-ray diffraction (XRD. The antibacterial activity was studied against Escherichia coli, Salmonella enterica, Proteus mirabilis, and Staphylococcus aureus by using well-diffusion method.

  14. Synthesis, characterization, crystal structure, DNA/BSA binding ability and antibacterial activity of asymmetric europium complex based on 1,10- phenanthroline

    Science.gov (United States)

    Alfi, Nafiseh; Khorasani-Motlagh, Mozhgan; Rezvani, Ali Reza; Noroozifar, Meissam; Molčanov, Krešimir

    2017-06-01

    A heteroleptic europium coordination compound formulated as [Eu(phen)2(OH2)2(Cl)2](Cl)(H2O) (phen = 1,10-phenanthroline), has been synthesized and characterized by elemental analysis, FT-IR spectroscopy, and single-crystal X-ray diffractometer. Crystal structure analysis reveals the complex is crystallized in orthorhombic system with Pca21 space group. Electronic absorption and various emission methods for investigation of the binding system of europium(III) complex to Fish Salmon deoxyribonucleic acid (FS-DNA) and Bovamin Serum Albumin (BSA) have been explored. Furthermore, the binding constants, binding sites and the corresponding thermodynamic parameters of the interaction system based on the van't Hoff equation for FS-DNA and BSA were calculated. The thermodynamic parameters reflect the exothermic nature of emission process (ΔH°<0 and ΔS°<0). The experimental results seem to indicate that the [Eu(phen)2(OH2)2(Cl)2](Cl)(H2O) bound to FS-DNA by non-intercalative mode which the groove binding is preferable mode. Also, the complex exhibits a brilliant antimicrobial activity in vitro against standard bacterial strains.

  15. Synthesis and characterization of silver doped hydroxyapatite nanocomposite coatings and evaluation of their antibacterial and corrosion resistance properties in simulated body fluid.

    Science.gov (United States)

    Mirzaee, Majid; Vaezi, Mohammadreza; Palizdar, Yahya

    2016-12-01

    Silver-doped hydroxyapatite (Ca10-xAgx(PO4)6(OH)2-x) films were synthesized and deposited on anodized titanium (Ti) using electrophoretic. The influence of different silver-dopant contents (X=0, 0.02, 0.05, 0.08 and 0.1) on the phase formation and microstructure of the powders were characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscope (XPS), and Fourier transform infrared spectrum analysis (FT-IR). XRD analysis confirmed the formation of Hexagonal structure of hydroxyapatite (HAp) annealed at 600°C with a small shift in the major peak position toward lower angles with adding silver. FT-IR spectroscopy disclosed the presence of the different vibrational modes matching to phosphates and hydroxyl groups and the absence of any band characteristics to silver. XPS analysis showed that 75% and 23% of silver was in the chemical states of Ag(2+) and Ag(+), respectively. However, only about 2% of silver was in the Ag(0) state, resulting in the high quality of nanocomposite films. The anodization treatment improves the bond strength between the Ag doped HAp deposited layers on TiO2. HAp and silver doped HAp (X=0.05) are regarded to be hydrophilic due to a large number of -OH groups on the surface. The sample with content of silver (x=0.05) also showed excellent antimicrobial efficacy (>99% reduction in viable cells). Electrochemical reveals the passive current densities of the HAp coated anodized Ti are lower than those of silver doped HAp coated anodized Ti, leading to a slightly lower corrosion resistance. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. In silico modeling and synthesis of phenyl and thienyl analogs of chalcones for potential leads as anti-bacterial agents

    Science.gov (United States)

    Kar, Swayamsiddha; Mishra, Rohit Kumar; Pathak, Ashutosh; Dikshit, Anupam; Golakoti, Nageswara Rao

    2018-03-01

    In the recent times, the common diseases like food poisoning, pneumonia, diarrhea etc. have been observed to be drug resistant. The present study deals with the synthesis of known chalcone derivatives using the Claisen-Schmidt condensation and further characterization using UV-vis, IR, 1H NMR, 13C NMR and mass spectrometry. These derivatives were first simulated for their anti-bacterial efficacy in silico and consequently confirmed in vitro to confirm the findings. One of the chalcones, 4-NDM-2‧-HC showed excellent in-vitro antibacterial activity with an IC90 0.43 mg/mL against Vibrio cholerae as compared to commercially available antibiotic gentamicin as the standard. Further, all these tested chalcone derivatives fulfill Lipinski's parameters and show tremendous drug likeness score, confirming their potential as antibacterial leads.

  17. Mechanism of plant-mediated synthesis of silver nanoparticles - A review on biomolecules involved, characterisation and antibacterial activity.

    Science.gov (United States)

    Rajeshkumar, S; Bharath, L V

    2017-08-01

    Engineering a reliable and eco-accommodating methodology for the synthesis of metal nanoparticles is a crucial step in the field of nanotechnology. Plant-mediated synthesis of metal nanoparticles has been developed as a substitute to defeat the limitations of conventional synthesis approaches such as physical and chemical methods. Biomolecules, such as proteins, amino acids, enzymes, flavonoids, and terpenoids from several plant extracts have been used as a stabilising and reducing agents for the synthesis of AgNPs. Regardless of an extensive range of biomolecules assistance in the synthesis procedure, researchers are facing a significant challenge to synthesise stable and geometrically controlled AgNPs. In the past decade, several efforts were made to develop Plant-mediated synthesis methods to produce stable, cost effective and eco-friendly AgNPs. More than hundred different plants extract sources for synthesising AgNPs were described in the last decade by several researchers. Most of the reviews were focused on various plant sources for synthesis, various characterization techniques for characteristic analysis, and antibacterial activity against bacterial. There are many reviews are available for the plant-mediated synthesis of AgNPs as well as antibacterial activity of AgNPs but this is the first review article mainly focused on biomolecules of plants and its various parts and operating conditions involved in the synthesis. Apart from, this review includes the characterisation of AgNPs and antibacterial activity of such nanoparticles with size, shape and method used for this study. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior

    Directory of Open Access Journals (Sweden)

    Shameli K

    2011-03-01

    Full Text Available Kamyar Shameli1, Mansor Bin Ahmad1, Mohsen Zargar2, Wan Md Zin Wan Yunus1, Abdolhossein Rustaiyan3, Nor Azowa Ibrahim11Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2Department of Biology, Faculty of Science, Islamic Azad University, Qom Branch, Qom, Iran; 3Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, IranAbstract: Silver nanoparticles (Ag NPs were synthesized by the chemical reducing method in the external and interlamellar space of montmorillonite (MMT as a solid support at room temperature. AgNO3 and NaBH4 were used as a silver precursor and reducing agent, respectively. The most favorable experimental conditions for synthesizing Ag NPs in the MMT are described in terms of the initial concentration of AgNO3. The interlamellar space limits changed little (d-spacing = 1.24–1.47 nm; therefore, Ag NPs formed on the MMT suspension with d-average = 4.19–8.53 nm diameter. The Ag/MMT nanocomposites (NCs, formed from AgNO3/MMT suspension, were characterizations with different instruments, for example UV-visible, PXRD, TEM, SEM, EDXRF, FT-IR, and ICP-OES analyzer. The antibacterial activity of different sizes of Ag NPs in MMT were investigated against Gram-positive, ie, Staphylococcus aureus and methicillin-resistant S. aureus (MRSA and Gram-negative bacteria, ie, Escherichia coli, Escherichia coli O157:H7, and Klebsiella pneumoniae, by the disk diffusion method using Mueller-Hinton agar (MHA. The smaller Ag NPs were found to have significantly higher antibacterial activity. These results showed that Ag NPs can be used as effective growth inhibitors in different biological systems, making them applicable to medical applications.Keywords: silver nanoparticles, nanoparticles, montmorillonite, antibacterial activity, Mueller-Hinton agar 

  19. Synthesis and Antibacterial Activity of Some New Phenothiazine Derivatives

    Directory of Open Access Journals (Sweden)

    Pawan Kumar Swarnkar

    2007-01-01

    Full Text Available A series of some new phenothiazine derivatives were synthesized with the objective for evaluation as antimicrobials. The title compounds were prepared by a five step synthesis scheme. 2-Amino-6-substituted benzothiazoles (1 on diazotization afford 6-substituted benzothiazolyl-2-diazonium chlorides (2. Reaction of 2 with cold solution of β-naphthol in dilute NaOH furnishes α-(2-diazo-6-substituted benzothiazolyl- β-sodionaphthoxides (3 which on acidification with concentrated HCl gives α-(2-diazo-6-substituted benzothiazolyl-β-naphthols (4. Reaction of 4 with p-substituted anilines gives α-(2-diazo-6-substituted benzothiazolyl-β-(p-substituted anilino naphthalenes (5. This synthesis besides by using conventional methods was also attempted using microwave. Fusion of 5 with sulphur in presence of iodine results in α-(2-diazo-6-substituted benzothiazolyl-6- substituted [2, 3-b] benzophenothiazines(6. The structures of all these compounds have been supported by elemental analysis and their spectral studies. All synthesized compounds were tested for their antibacterial activity using standard drugs.

  20. Synthesis, antibacterial and antifungal activity of some derivatives of ...

    Indian Academy of Sciences (India)

    Unknown

    -4-one; antibacterial ..... Kanamycin was used as standard antibiotic for antibacterial activities. Nutrient agar (NA) was used as ... (mould) and Penicillium sp. (blue mould). Antifun- gal activity was assessed by the poisoned food tech- nique,17.

  1. Synthesis and antibacterial activity of monocyclic 3-carboxamide tetramic acids

    Directory of Open Access Journals (Sweden)

    Yong-Chul Jeong

    2013-09-01

    Full Text Available A chemical library of carboxamide-substituted tetramates designed by analogy with antibacterial natural products, a method for their rapid construction, and the evaluation of their antibacterial activity is reported.

  2. Synthesis and biological evaluation of benzazepine oxazolidinone antibacterials.

    Science.gov (United States)

    Johnson, Paul D; Aristoff, Paul A; Zurenko, Gary E; Schaadt, Ronda D; Yagi, Betty H; Ford, Charles W; Hamel, Judith C; Stapert, Douglas; Moerman, Judy K

    2003-12-01

    Novel benzazepine oxazolidinone antibacterials were synthesized and evaluated against clinically relevant susceptible and resistant organisms. The effect of ring nitrogen position and N-substitution on antibacterial activity is examined.

  3. One-step synthesis of silver nanoparticles in an aqueous solution and their antibacterial activities

    Science.gov (United States)

    Zhang, Guangyu; Zhang, Feng; Morikawa, Hideaki; Chen, Yueyue

    2014-03-01

    A one-step simple synthesis of silver colloid nanoparticles with controllable sizes is presented in this research. In the synthesis, an amino-terminated hyperbranched polymer (HBP-NH2) was applied as a stabilizer and a reductant. The syntheses, performed at various initial AgNO3 concentrations (0.28-0.56 g/l) in a 2 g/l HBP-NH2 aqueous solution, produced silver colloid nanoparticles having average sizes from 3 to 30 nm with narrow size distributions. The formation of silver colloid nanoparticles was characterized by Fourier Transform Infrared Spectrophotometry (FTIR), Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), UV/Visible Absorption Spectrophotometry, and X-ray Diffraction (XRD) measurements. The results indicated that both particle size and the UV absorption are strongly dependent on the initial AgNO3 concentrations. The silver colloid nanoparticles, prepared with a 0.35 g/l AgNO3 aqueous solution in the presences of 2 g/l HBP-NH2, showed good antibacterial activities against Gram-negative bacteria ( Escherichia coli) and Gram-positive bacteria ( Staphylococcus aureus). A very low concentration of nano-silver (as low as 3.0 ug/ml Ag) also gave excellent antibacterial performance.

  4. synthesis, characterization, antibacterial and antifungal studies

    African Journals Online (AJOL)

    Preferred Customer

    Ce(III) ions. The IR spectral data reveal that the ligand behaves as bidentate with ON donor atom sequence towards central metal ion. The molar conductance values of metal ... and kinetic parameters calculated by Coats-Redfern method are suggestive of more ordered activated state in ..... concentration of metal complex.

  5. Synthesis, characterization and antibacterial evaluation of some ...

    African Journals Online (AJOL)

    NMR, 13C-NMR, mass spectrometry and elemental analysis and screened for antimicrobial activity against two Gram-positive strains (Staphylococcus aureus and Bacillus subtilis) and two ... DOI: http://dx.doi.org/10.4314/bcse.v28i3.14 ...

  6. Synthesis, Characterization, Antibacterial, α-Glucosidase Inhibition ...

    African Journals Online (AJOL)

    The off-white precipitates were collected by filtration, washed with distilled water and dried to acquire the compound 3. ... progress of reaction was monitored by TLC. The product was precipitated by adding cold water, filtered, washed with ...... Micellar chromatographic procedure with direct injection for the determination of.

  7. Synthesis, characterization, antibacterial and antifungal studies of ...

    African Journals Online (AJOL)

    The analytical data of these metal complexes showed metal:ligand ratio of 1:2. The physico-chemical study supports the presence of square planar geometry around Cu(II) and octahedral geometry around Mn(II), Co(II), La(III) and Ce(III) ions. The IR spectral data reveal that the ligand behaves as bidentate with ON donor ...

  8. Synthesis and Antibacterial Activities of Some Schiff Bases

    Directory of Open Access Journals (Sweden)

    Mohamed N. Ibrahim

    2011-01-01

    Full Text Available Schiff bases p-hydroxybenzylidene-2-carboxyaniline, p-nitrobenz-ylidene-2-carboxyaniline, p-(N, N-dimethylaminobenzylidene-2-carboxyaniline, N-(4-hydroxybezylidene-benzene-1,2-diamine, N--(4-nitrobezylidenebenzene-1,2-diamine, N-(4-(N, N-dimethylaminobezylidenebenzene-1,2-diamine, N-(4-(N,N-dimethylaminobenzylidenenaphthalen-1-amine,N-(4-nitrobenzylidenenaphthalen-1-amine,N--(4-chlorobenzylidenenaphthalen-1-amine,sodium-4-(4-(N,N-dimethyl aminobenzylideneaminonaphthalene-1-sulfonate,sodium -4-(4-nitrobenzylidene-aminonaphthalene-1-sulfonate and sodium-4-(4-chlorobenzylideneamino naphthalene-1-sulfonate obtained by condensation of aniline and naphthyl-amine derivatives with some aromatic aldehydes were characterized by physical and spectral methods. The biological activity of these products were as antibacterial agents against three species of human pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Klebsiella sp. Nearly 50% of these compounds showed reasonable activity against the bacterial species investigated and we found that the antibacterial activity is dependent on the molecular structure of the compounds.

  9. Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities.

    Science.gov (United States)

    Din, Muhammad Imran; Arshad, Farhan; Hussain, Zaib; Mukhtar, Maria

    2017-12-28

    Copper nanoparticles (CuNPs) are of great interest due to their extraordinary properties such as high surface-to-volume ratio, high yield strength, ductility, hardness, flexibility, and rigidity. CuNPs show catalytic, antibacterial, antioxidant, and antifungal activities along with cytotoxicity and anticancer properties in many different applications. Many physical and chemical methods have been used to synthesize nanoparticles including laser ablation, microwave-assisted process, sol-gel, co-precipitation, pulsed wire discharge, vacuum vapor deposition, high-energy irradiation, lithography, mechanical milling, photochemical reduction, electrochemistry, electrospray synthesis, hydrothermal reaction, microemulsion, and chemical reduction. Phytosynthesis of nanoparticles has been suggested as a valuable alternative to physical and chemical methods due to low cytotoxicity, economic prospects, environment-friendly, enhanced biocompatibility, and high antioxidant and antimicrobial activities. The review explains characterization techniques, their main role, limitations, and sensitivity used in the preparation of CuNPs. An overview of techniques used in the synthesis of CuNPs, synthesis procedure, reaction parameters which affect the properties of synthesized CuNPs, and a screening analysis which is used to identify phytochemicals in different plants is presented from the recent published literature which has been reviewed and summarized. Hypothetical mechanisms of reduction of the copper ion by quercetin, stabilization of copper nanoparticles by santin, antimicrobial activity, and reduction of 4-nitrophenol with diagrammatic illustrations are given. The main purpose of this review was to summarize the data of plants used for the synthesis of CuNPs and open a new pathway for researchers to investigate those plants which have not been used in the past. Graphical abstract Proposed Mechanism for Antibacterial activity of copper nanoparticles.

  10. SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ...

    African Journals Online (AJOL)

    Preferred Customer

    ABSTRACT. A series of new biologically active complexes of Zn(II), Cu(II), Co(II) and Ni(II) with imidazole derivative have been synthesized. The synthesized chelating agent and metal(II) complexes were screened for antibacterial activities against four pathogenic species of bacteria namely; Eschereschi coli, Pseudomonas.

  11. Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities

    International Nuclear Information System (INIS)

    Chudasama, Bhupendra; Vala, Anjana K.; Andhariya, Nidhi; Mehta, R. V.; Upadhyay, R. V.

    2010-01-01

    In this article, we describe a simple one-pot rapid synthesis route to produce uniform silver nanoparticles by thermal reduction of AgNO 3 using oleylamine as reducing and capping agent. To enhance the dispersal ability of as-synthesized hydrophobic silver nanoparticles in water, while maintaining their unique properties, a facile phase transfer mechanism has been developed using biocompatible block co-polymer pluronic F-127. Formation of silver nanoparticles is confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM) and UV-vis spectroscopy. Hydrodynamic size and its distribution are obtained from dynamic light scattering (DLS). Hydrodynamic size and size distribution of as-synthesized and phase transferred silver nanoparticles are 8.2 ± 1.5 nm (σ = 18.3%) and 31.1 ± 4.5 nm (σ = 14.5%), respectively. Antimicrobial activities of hydrophilic silver nanoparticles is tested against two Gram positive (Bacillus megaterium and Staphylococcus aureus), and three Gram negative (Escherichiacoli, Proteusvulgaris and Shigellasonnei) bacteria. Minimum inhibitory concentration (MIC) values obtained in the present study for the tested microorganisms are found much better than those reported for commercially available antibacterial agents.

  12. Synthesis, antibacterial and cytotoxic activity evaluation of hydroxyurea derivatives.

    Science.gov (United States)

    Kos, Ivan; Jadrijević-Mladar, Milena; Butula, Ivan; Biruš, Mladen; Maravić-Vlahoviček, Gordana; Dabelić, Sanja

    2013-06-01

    5 Synthesis and biological evaluation of a series (N = 16) of cyclic and acyclic hydroxyurea derivatives, including benzotriazole-, isocyanuric acid- and biuret-containing compounds, are disclosed. 1-N-(benzyloxycarbamoyl)benzotriazole was used as a benzyloxyisocyanate donor, a useful intermediate in the preparation of substituted hydroxyurea. Antibacterial activities of synthesized hydroxyurea derivatives were tested on three E. coli strains, i.e., a strain susceptible to antibiotics, a strain resistant to macrolide antibiotics and a strain resistant to aminoglycoside antibiotics. Six compounds (three acyclic and three cyclic hydroxyureas) showed growth inhibition of the tested E. coli strains, with different specificity toward each strain. Results of the cytotoxic activity evaluation revealed that twelve out of sixteen test compounds were cytotoxic to human acute monocytic leukemia THP-1 and/or human acute T cell leukemia Jurkat cell line. 1-(N-hydroxycarbamoyl) benzotriazole () increased the metabolic activity of both cell lines. Two compounds, 1-(N-hydroxycarbamoyl) benzotriazole (5) and N,N',N''-trihydroxybiuret (15), were identified as potential NO donors.

  13. Green synthesis and antibacterial effect of silver nanoparticles using Eucalyptus camaldulensis

    Directory of Open Access Journals (Sweden)

    Samaneh Dolatabadi

    2017-11-01

    Full Text Available Introduction and Aims: Due to the developing bacterial drug resistance to classical antimicrobial agents, it seems necessary to produce an innovative therapeutic approach to eliminate resistant pathogens. This study aimed to biosynthesis of silver nanoparticles (AgNPs using flowers extract of Eucalyptus camaldulensis and determined of antibacterial activity of produced AgNPs.  Materials and Methods: We used an environmentally friendly extracellular biosynthetic technique for the production of the AgNPs. The reducing agents used to produce the nanoparticles were from the aqueous flowers extract of Eucalyptus camaldulensis. The AgNPs were characterized by color changes, the UV-visible spectroscopy, FTIR analysis, XRD patterns and Scanning Electron Microscopy (SEM technique. The antibacterial activity of AgNPs was investigated against Gram-positive and Gram-negative bacteria ,through broth micro dilution (MIC values and MBC (Minimum bactericidal concentration assays. Results: An absorption peak at 413 nm and the color change to dark brown were corresponding to the plasmon absorbance of AgNPs and then were performed by FTIR and XRD methods. The average size of nanoparticles was observed between 67 and 80 nm. Furthermore, the MIC value of extract against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa were 3.12, 6.25, and 50 mg/mL, respectively. The corresponding MBC values were 6.25, 6.25 and 100 mg/mL, respectively. Conclusion: Our findings confirmed that extracellular synthesis of AgNPs mediated by E. camaldulensis flowers extract indicated a good bactericidal activity against the tested bacteria and can be used in various fields as an antibacterial agent. Further studies are needed to characterize the toxicity effect of these particles.

  14. Synthesis and characterization of cloisite-30B clay dispersed poly (acryl amide/sodium alginate)/AgNp hydrogel composites for the study of BSA protein drug delivery and antibacterial activity

    Science.gov (United States)

    Nanjunda Reddy, B. H.; Ranjan Rauta, Pradipta; Venkatalakshimi, V.; Sreenivasa, Swamy

    2018-02-01

    The aim of this research is to inspect the effect of Cloisite-30B (C30B) modified clay dispersed poly (acrylamide-co-Sodiumalginate)/AgNp hydrogel nanocomposites (PASA/C30B/Ag) for drug delivery and antibacterial activity. A novel hydrogel composite based sodium alginate (SA) and the inorganic modified clay with silver nano particle (C30B/AgNps)polymer hydrogel composites are synthesized via the graft copolymerization of acrylamide (AAm) in an aqueous medium with methylene bisacrylamide (MBA) as a crosslinking agent and ammonium per sulfate(APS) as an initiator. The UV/Visible spectroscopy of obtained composites is successfully studied, which confirms the occurrence of AgNps in the hydrogel composites. And the swelling capacity and bovine serum albumin (BSA) protein as model drug delivery study for these hydrogel nanocomposites have been carried out. The C30B/Ag filled hydrogel composites exhibit superior water absorbency or swelling capacity compared to pure samples and it is establish that the formulations with clay (C30B) dispersed silver nanocomposite hydrogels show improved and somewhat faster rate of drug delivery than other formulations(pure systems) and SEM and TEM reports suggests that the size of AgNps in the composite hydrogels is in the range of 5-10 nm with shrunken surface and the antibacterial characterizations for gram positive and gram negative bacteria are carried out by using Streptococcus faecalis (S. Faecalis) and Escherichia coli (E.coli) as model bacteria and the hydrogel composites of PASA/C30B/Ag shows exceptional antibacterial activity against both the bacteria as compared to pure hydrogel composites samples.

  15. Similarity analysis, synthesis, and bioassay of antibacterial cyclic peptidomimetics

    Directory of Open Access Journals (Sweden)

    Workalemahu M. Berhanu

    2012-07-01

    Full Text Available The chemical similarity of antibacterial cyclic peptides and peptidomimetics was studied in order to identify new promising cyclic scaffolds. A large descriptor space coupled with cluster analysis was employed to digitize known antibacterial structures and to gauge the potential of new peptidomimetic macrocycles, which were conveniently synthesized by acylbenzotriazole methodology. Some of the synthesized compounds were tested against an array of microorganisms and showed antibacterial activity against Bordetella bronchistepica, Micrococcus luteus, and Salmonella typhimurium.

  16. Synthesis and antibacterial activity of new chiral N ...

    Indian Academy of Sciences (India)

    negative bacteria; Escherichia. Coli, Klebsiella pneumonieae, Acinetobacter, Pseudomonas aeruginosa, Enterococcus, Salmonella sp. The compounds showed moderate to good antibacterial activity. Keywords. Oxazolidinone; chlorosulfonyl ...

  17. Ternary complexes of Zn(II) and Cu(II) with 1-((2-hydroxynaphthalen-1-yl)methylene)-4-phenylthiosemicarbazide in the presence of heterocyclic bases as auxiliary ligands: Synthesis, spectroscopic and structural characterization and antibacterial activity

    Science.gov (United States)

    Azarkish, Mohammad; Akbari, Alireza; Sedaghat, Tahereh; Simpson, Jim

    2018-03-01

    The new ternary complexes, ZnLL‧ [L = 1-((2-hydroxynaphthalen-1-yl)methylene)-4-phenylthiosemicarbazide and L‧ = imidazole (1), 2, 2‧-bipyridine (2) and 2-methyimidazole (3)], Zn2L2L‧ [L‧ = 4, 4‧-bipy (4)] and CuLL‧ [L‧ = 2, 2‧-bipy (5)] have been synthesized by the reaction of a metal(II) acetate salt with the thiosemicarbazone and in presence of heterocyclic bases as auxiliary ligands. The synthesized compounds were investigated by elemental analysis and IR, 1H NMR, and 13C NMR spectroscopy and complex 5 was structurally characterized by X-ray crystallography. The results indicate the thiosemicarbazone doubly deprotonated and coordinates to metal through the thiolate sulfur, imine nitrogen and phenolic oxygen atoms. The nitrogen atom(s) of the auxiliary ligand complete the coordination sphere. Complex 4 is binuclear with 4, 4‧-bipy acting as a bridging ligand. The structure of 5 is a distorted square pyramid with one of the bipyridine nitrogen atoms in the apical position. This compound creates an inversion dimer in solid state by intermolecular hydrogen bonds of Nsbnd H⋯S type. The in vitro antibacterial activity of the synthesized compounds were evaluated against Gram-positive (B. subtilis and S. aureus) and Gram-negative (P. aeruginosa) bacteria and is compared to that of standard antibacterial drugs. All complexes exhibit good inhibitory effects and are significantly more effective than the parent ligand.

  18. Five-Coordinate Zinc(II Complex: Synthesis, Characterization, Molecular Structure, and Antibacterial Activities of Bis-[(E-2-hydroxy-N′-{1-(4-methoxyphenylethylidene}benzohydrazido]dimethylsulfoxidezinc(II Complex

    Directory of Open Access Journals (Sweden)

    Zhi-Qiang Liu

    2017-01-01

    Full Text Available The titled Zn(II complex was synthesized by reacting the compound (E-2-hydroxy-N′-{1-(4-methoxyphenylethylidene}benzohydrazide with zinc(II acetate dihydrate in alkaline DMSO and ethanol solution under reflux condition for 28 hours. The resulting solid was filtered and recrystallized from the mixture of ethanol and DMSO. The hydrazone Schiff base and its Zn(II complex were characterized using 1H, 13C NMR, FTIR, UV-Vis spectroscopy, and single crystal X-ray diffraction analysis. Meanwhile, their antibacterial activities were examined using disc diffusion method. The spectral studies showed that the hydrazone Schiff base underwent keto-enol tautomerization, forming a bidentate ligand (N,O towards Zn(II ion. Surprisingly, on top of the two hydrazone Schiff base molecules which coordinated to the Zn metal center, an additional DMSO molecule was found attached to the Zn metal center in the crystal data, resulting in a 5-coordinate distorted trigonal bipyramidal Zn(II complex. Both hydrazone Schiff base and its Zn(II complexes were found to exhibit low antibacterial activity even when the concentrations were increased to 800 ppm.

  19. Green synthesis of silver nanoparticles using Delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities

    Science.gov (United States)

    Suresh, Gopal; Gunasekar, Poosali Hariharan; Kokila, Dhanasegaran; Prabhu, Durai; Dinesh, Devadoss; Ravichandran, Nagaiya; Ramesh, Balasubramanian; Koodalingam, Arunagirinathan; Vijaiyan Siva, Ganesan

    2014-06-01

    Green synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Delphinium denudatum (Dd) by reduction of Ag+ ions from silver nitrate solution has been investigated. The synthesized DdAgNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), Field emission scanning electron microscope (FESEM) and Fourier transform infrared spectroscopy (FTIR). The prepared DdAgNPs showed maximum absorbance at 416 nm and particles were polydispersed in nature, spherical in shape and the size of the particle obtained was ⩽85 nm. The DdAgNPs exhibited antibacterial activity against Staphylococcus aureus ATCC 6538, Bacillus cereus NCIM 2106, Escherichia coli ATCC 8739 and Pseudomonas aeruginosa ATCC 9027. The DdAgNPs showed potent larvicidal activity against second instar larvae of dengue vector Aedes aegypti with a LC50 value of 9.6 ppm.

  20. Synthesis of silver nanoparticles using flavonoids: hesperidin, naringin and diosmin, and their antibacterial effects and cytotoxicity

    Science.gov (United States)

    Sahu, Nidhi; Soni, Deepika; Chandrashekhar, B.; Satpute, D. B.; Saravanadevi, Sivanesan; Sarangi, B. K.; Pandey, R. A.

    2016-07-01

    Three different flavonoids -hesperidin, naringin and diosmin (constituents of citrus plants) were used for the synthesis of silver nanoparticles (AgNPs). Aqueous solutions of pure flavonoids (0.2 mg mL-1) mixed with 1 mM AgNO3 solution were exposed to bright sunlight to prepare the nanoparticles. Characterization of the synthesized nanoparticles by UV-Visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy revealed that the synthesized silver nanoparticles were 10-80 nm in size and polydispersed in nature. Bactericidal effect against common pathogens and cytotoxicity of the synthesized silver nanoparticles was investigated on human promyelocytic leukemic (HL-60) cells. It is concluded that AgNPs synthesized using Naringin as reducing agent showed higher stability and better antibacterial and cytotoxic activities.

  1. Systematic and Stereoselective Total Synthesis of Mannosylerythritol Lipids and Evaluation of Their Antibacterial Activity.

    Science.gov (United States)

    Nashida, Junki; Nishi, Nobuya; Takahashi, Yoshiaki; Hayashi, Chigusa; Igarashi, Masayuki; Takahashi, Daisuke; Toshima, Kazunobu

    2018-03-13

    The total synthesis of the 20 homogeneous members of mannosylerythritol lipids (MELs) with different alkyl chain lengths was effectively and systematically accomplished from a strategically designed common key intermediate that was stereoselectively constructed by the borinic acid catalyzed β-mannosylation reaction. In addition, their antibacterial activities against Gram-positive bacteria were evaluated. Our results demonstrated that not only the length of the alkyl chains but also the pattern of Ac groups on the mannose moiety were important factors for antibacterial activity.

  2. Synthesis and antibacterial study of some s-substituted aliphatic ...

    African Journals Online (AJOL)

    Antibacterial activity was evaluated against five bacterial strains, namely, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, with ciprofloxacin used as standard antibacterial agent. Results: Out of nine synthesized derivatives, compound 7a was the most active ...

  3. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles.

    Science.gov (United States)

    Patil, Maheshkumar Prakash; Kim, Gun-Do

    2017-01-01

    This review covers general information about the eco-friendly process for the synthesis of silver nanoparticles (AgNP) and gold nanoparticles (AuNP) and focuses on mechanism of the antibacterial activity of AgNPs and the anticancer activity of AuNPs. Biomolecules in the plant extract are involved in reduction of metal ions to nanoparticle in a one-step and eco-friendly synthesis process. Natural plant extracts contain wide range of metabolites including carbohydrates, alkaloids, terpenoids, phenolic compounds, and enzymes. A variety of plant species and plant parts have been successfully extracted and utilized for AgNP and AuNP syntheses. Green-synthesized nanoparticles eliminate the need for a stabilizing and capping agent and show shape and size-dependent biological activities. Here, we describe some of the plant extracts involved in nanoparticle synthesis, characterization methods, and biological applications. Nanoparticles are important in the field of pharmaceuticals for their strong antibacterial and anticancer activity. Considering the importance and uniqueness of this concept, the synthesis, characterization, and application of AgNPs and AuNPs are discussed in this review.

  4. Synthesis of Silver nanoparticles (AgNPs) with Antibacterial Activity

    Science.gov (United States)

    Campillo Gloria, E.; Ederley, Vélez; Gladis, Morales; César, Hincapié; Jaime, Osorio; Oscar, Arnache; Uribe José, Ignacio; Franklin, Jaramillo

    2017-06-01

    The synthesis of nanomaterials is currently one of the most active in nanoscience branches; especially those help improve the human quality life. Silver nanoparticles (AgNPs) are an example of this as it is known to have inhibitory and bactericidal effects. In this work, we report the synthesis of silver nanoparticles by chemical reduction method of silver nitrate (AgNO3) from aqueous solution, using a mix of polivinyl pyrrolidone (PVP) - Aloe Vera as reducing agent and for stabilization and control of particle size. Silver nanoparticles obtained were characterized by Scanning Electron Microscopy (SEM), UV-visible spectroscopy and measurements using Zetasizer Nano ZS were applied to size estimation. The existence of surface plasmon resonance peak at λmax ~ 420 nm is evidence of silver nanoparticles formation. It was possible to standardize an appropriate protocol for the evaluation of bactericidal activity of the nanoparticles, for mesophilic microorganisms. Bactericidal activity above 90% against these kinds of bacteria was demonstrated.

  5. Synthesis of Silver nanoparticles (AgNPs) with Antibacterial Activity

    International Nuclear Information System (INIS)

    Gloria, E. Campillo; Ederley, Vélez; César, Hincapié; Gladis, Morales; Jaime, Osorio; Oscar, Arnache; José, Ignacio Uribe; Franklin, Jaramillo

    2017-01-01

    The synthesis of nanomaterials is currently one of the most active in nanoscience branches; especially those help improve the human quality life. Silver nanoparticles (AgNPs) are an example of this as it is known to have inhibitory and bactericidal effects. In this work, we report the synthesis of silver nanoparticles by chemical reduction method of silver nitrate (AgNO 3 ) from aqueous solution, using a mix of polivinyl pyrrolidone (PVP) – Aloe Vera as reducing agent and for stabilization and control of particle size. Silver nanoparticles obtained were characterized by Scanning Electron Microscopy (SEM), UV–visible spectroscopy and measurements using Zetasizer Nano ZS were applied to size estimation. The existence of surface plasmon resonance peak at λ max ∼ 420 nm is evidence of silver nanoparticles formation. It was possible to standardize an appropriate protocol for the evaluation of bactericidal activity of the nanoparticles, for mesophilic microorganisms. Bactericidal activity above 90% against these kinds of bacteria was demonstrated. (paper)

  6. In Situ Synthesis of Silver Nanoparticles on the Polyelectrolyte-Coated Sericin/PVA Film for Enhanced Antibacterial Application.

    Science.gov (United States)

    Cai, Rui; Tao, Gang; He, Huawei; Guo, Pengchao; Yang, Meirong; Ding, Chaoxiang; Zuo, Hua; Wang, Lingyan; Zhao, Ping; Wang, Yejing

    2017-08-18

    To develop silk sericin (SS) as a potential antibacterial biomaterial, a novel composite of polyelectrolyte multilayers (PEMs) coated sericin/poly(vinyl alcohol) (SS/PVA) film modified with silver nanoparticles (AgNPs) has been developed using a layer-by-layer assembly technique and ultraviolet-assisted AgNPs synthesis method. Ag ions were enriched by PEMs via the electrostatic attraction between Ag ions and PEMs, and then reduced to AgNPs in situ with the assistance of ultraviolet irradiation. PEMs facilitated the high-density growth of AgNPs and protected the synthesized AgNPs due to the formation of a 3D matrix, and thus endowed SS/PVA film with highly effective and durable antibacterial activity. Scanning electron microscopy, energy dispersive spectroscopy, X-ray diffractometry, Fourier transfer infrared spectroscopy, water contact angle, mechanical property and thermogravimetric analysis were applied to characterize SS/PVA, PEMs-SS/PVA and AgNPs-PEMs-SS/PVA films, respectively. AgNPs-PEMs-SS/PVA film has exhibited good mechanical performance, hydrophilicity, water absorption capability as well as excellent and durable antibacterial activity against Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa and good stability and degradability. This study has developed a simple method to design and prepare AgNPs-PEMs-SS/PVA film for potential antibacterial application.

  7. Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity.

    Science.gov (United States)

    Sadeghi, Babak; Rostami, Amir; Momeni, S S

    2015-01-05

    In the present work, we describe the synthesis of silver nanoparticles (Ag-NPs) using seed aqueous extract of Pistacia atlantica (PA) and its antibacterial activity. UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infra red spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer (EDAX) were performed to ascertain the formation of Ag-NPs. It was observed that the growths of Ag-NPs are stopped within 35 min of reaction time. The synthesized Ag-NPs were characterized by a peak at 446 nm in the UV-visible spectrum. XRD confirmed the crystalline nature of the nanoparticles of 27 nm size. The XRD peaks at 38°, 44°, 64° and 77° can be indexed to the (111), (200), (220) and (311) Bragg's reflections of cubic structure of metallic silver, respectively. The FTIR result clearly showed that the extracts containing OH as a functional group act in capping the nanoparticles synthesis. Antibacterial activities of Ag-NPs were tested against the growth of Gram-positive (S. aureus) using SEM. The inhibition was observed in the Ag-NPs against S. aureus. The results suggest that the synthesized Ag-NPs act as an effective antibacterial agent. It is confirmed that Ag-NPs are capable of rendering high antibacterial efficacy and hence has a great potential in the preparation of used drugs against bacterial diseases. The scanning electron microscopy (SEM), indicated that, the most strains of S. aureus was damaged and extensively disappeared by addition of Ag-NPs. The results confirmed that the (PA) is a very good eco friendly and nontoxic source for the synthesis of Ag-NPs as compared to the conventional chemical/physical methods. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity

    Science.gov (United States)

    Sadeghi, Babak; Rostami, Amir; Momeni, S. S.

    2015-01-01

    In the present work, we describe the synthesis of silver nanoparticles (Ag-NPs) using seed aqueous extract of Pistacia atlantica (PA) and its antibacterial activity. UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infra red spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer (EDAX) were performed to ascertain the formation of Ag-NPs. It was observed that the growths of Ag-NPs are stopped within 35 min of reaction time. The synthesized Ag-NPs were characterized by a peak at 446 nm in the UV-visible spectrum. XRD confirmed the crystalline nature of the nanoparticles of 27 nm size. The XRD peaks at 38°, 44°, 64° and 77° can be indexed to the (1 1 1), (2 0 0), (2 2 0) and (3 1 1) Bragg's reflections of cubic structure of metallic silver, respectively. The FTIR result clearly showed that the extracts containing OH as a functional group act in capping the nanoparticles synthesis. Antibacterial activities of Ag-NPs were tested against the growth of Gram-positive (S. aureus) using SEM. The inhibition was observed in the Ag-NPs against S. aureus. The results suggest that the synthesized Ag-NPs act as an effective antibacterial agent. It is confirmed that Ag-NPs are capable of rendering high antibacterial efficacy and hence has a great potential in the preparation of used drugs against bacterial diseases. The scanning electron microscopy (SEM), indicated that, the most strains of S. aureus was damaged and extensively disappeared by addition of Ag-NPs. The results confirmed that the (PA) is a very good eco friendly and nontoxic source for the synthesis of Ag-NPs as compared to the conventional chemical/physical methods.

  9. Synthesis and comparing the antibacterial activities of pyrimidine ...

    Indian Academy of Sciences (India)

    triazol-3-yl)-6-methyl-4- phenyl pyrimidin-2(1H)-one have been synthesized. Among the synthesized derivatives, triazole substituted compounds have shown higher antibacterial inhibition when compared to the thiadiazole derivatives. All the.

  10. Synthesis and comparative antibacterial studies of some benzylidene monosaccharide benzoates

    Directory of Open Access Journals (Sweden)

    Mohammed Matin

    2015-03-01

    Full Text Available Some 4,6-O-benzylidene protected 2,3-di-O-benzoates of methyl a-D-glucopyranoside and methyl a-D-mannopyranoside were prepared. All the compounds (1-7 were screened for in vitro antibacterial activity study against ten human pathogenic bacteria. The study revealed that the benzoylated mannopyranosides (5-7 are more prone towards antibacterial functionalities than that of the glucopyranosides (2-3.

  11. Synthesis of silane ligand-modified graphene oxide and antibacterial activity of modified graphene-silver nanocomposite.

    Science.gov (United States)

    Fathalipour, Soghra; Mardi, Mojtaba

    2017-10-01

    In this research, a new type of chemically modified graphene oxide (GO) was synthesized based a silane ligand and then used as substrate and stabilizing for the synthesis of monodispersed and small Ag nanoparticles (NPs). First, ligand molecules were successfully grafted onto the surface of GO (LGO) and then, active groups of LGO could effectively interact with Ag ions. The reduction of Ag ions and LGO sheets was carried out by hydrazine under reflux. The resulted nanocomposite was fully characterized by different techniques. Furthermore, the antibacterial behavior of nanocomposite was studied against E. coli and S. aureus. The results showed that nanocomposite exhibits good antibacterial activity against E. coli and S. aureus and also S. aureus showed greater resistance than the E. coli strains against the LG/Ag nanocomposite. Copyright © 2017. Published by Elsevier B.V.

  12. Design, synthesis and antibacterial evaluation of honokiol derivatives.

    Science.gov (United States)

    Wu, Bo; Fu, Su-Hong; Tang, Huan; Chen, Kai; Zhang, Qiang; Peng, Ai-Hua; Ye, Hao-Yu; Cheng, Xing-Jun; Lian, Mao; Wang, Zhen-Ling; Chen, Li-Juan

    2018-02-15

    Staphylococcus aureus is a major and dangerous human pathogen that causes a range of clinical manifestations of varying severity, and is the most commonly isolated pathogen in the setting of skin and soft tissue infections, pneumonia, suppurative arthritis, endovascular infections, foreign-body associated infections, septicemia, osteomyelitis, and toxic shocksyndrome. Honokiol, a pharmacologically active natural compound derived from the bark of Magnolia officinalis, has antibacterial activity against Staphylococcus aureus which provides a great inspiration for the discovery of potential antibacterial agents. Herein, honokiol derivatives were designed, synthesized and evaluated for their antibacterial activity by determining the minimum inhibitory concentration (MIC) against S. aureus ATCC25923 and Escherichia coli ATCC25922 in vitro. 7c exhibited better antibacterial activity than other derivatives and honokiol. The structure-activity relationships indicated piperidine ring with amino group is helpful to improve antibacterial activity. Further more, 7c showed broad spectrum antibacterial efficiency against various bacterial strains including eleven gram-positive and seven gram-negative species. Time-kill kinetics against S. aureus ATCC25923 in vitro revealed that 7c displayed a concentration-dependent effect and more rapid bactericidal kinetics better than linezolid and vancomycin with the same concentration. Gram staining assays of S. aureus ATCC25923 suggested that 7c could destroy the cell walls of bacteria at 1×MIC and 4×MIC. Copyright © 2017. Published by Elsevier Ltd.

  13. Biogenic synthesis of silver nanoparticles using guava ( Psidium guajava) leaf extract and its antibacterial activity against Pseudomonas aeruginosa

    Science.gov (United States)

    Bose, Debadin; Chatterjee, Someswar

    2016-08-01

    Among the various inorganic nanoparticles, silver nanoparticles have received substantial attention in the field of antimicrobial research. For safe and biocompatible use of silver nanoparticles in antimicrobial research, the different biogenic routes are developed to synthesize silver nanoparticles that do not use toxic chemicals. Among those, to synthesize silver nanoparticles, the use of plant part extract becomes an emerging field because plant part acts as reducing as well as capping agent. For large-scale production of antibacterial silver nanoparticles using plant part, the synthesis route should be very simple, rapid, cost-effective and environment friendly based on easy availability and non-toxic nature of plant, stability and antibacterial potential of biosynthesized nanoparticles. In the present study, we report a very simple, rapid, cost-effective and environment friendly route for green synthesis of silver nanoparticles using guava ( Psidium guajava) leaf extract as reducing as well as capping agent. This plant has been opted for the present study for its known medicinal properties, and it is easily available in all seasons and everywhere. The biosynthesized silver nanoparticles are characterized by UV-Vis and TEM analysis. The average particle size is 40 nm in the range of 10-90 nm. The antibacterial activity of these nanoparticles against Pseudomonas aeruginosa MTCC 741 has been measured by disc diffusion method, agar cup assay and serial dilution turbidity measurement assay. The results show that green synthesized silver nanoparticles, using guava ( Psidium guajava) leaf extract, have a potential to inhibit the growth of bacteria.

  14. Preparation, characterization and antibacterial effects of eco-friendly ...

    African Journals Online (AJOL)

    Purpose: To synthesize and characterize eco-friendly gold nanorods (Au-NRs) and to assess their effects against two bacterial strains. Methods: Synthesis of eco-friendly gold nanorods was done from an aqueous solution of chloroauric acid and cetyltrimethylammonium bromide by mixing Olea europaea fruit and Acacia ...

  15. Formulation and Characterization of Antibacterial Fluoride-releasing Sealants

    Science.gov (United States)

    Fan, Yuwei; Townsend, Janice; Wang, Yapin; Lee, Eun Chee; Evans, Katie; Hender, Erica; Hagan, Joseph L.; Xu, Xiaoming

    2013-01-01

    Purpose The purpose of this study was to formulate and characterize experimental antibacterial fluoride-releasing sealants and compare them with commercial sealants for fluoride release, recharge, adhesion, and microleakage. Methods Two experimental sealants (Exp-1, Exp-2) containing a synthesized antibacterial fluoride-releasing monomer and fluoride-releasing filler were formulated. Exp-2 also contained NovaMin nanoparticles. Commercial sealants Clinpro (CL) FluoroShield (FS), and SeLECT Defense (E34) were also included. Fluoride release from disk samples in deionized water was measured daily using an ion-selective electrode for 14 days, and after recharging with Neutra-Foam (2.0% sodium fluoride), fluoride was measured for 5 days. Microtensile bonding strengths (MTBS) to enamel were tested after 24-hour storage in water at 37°C or thermocycling 5-55°C for 1,000 cycles. A microleakage test was conducted on extracted teeth using a dye-penetration method. The data were analyzed using analysis of variance with the Tukey’s honestly significant difference test and Kruskal-Wallis test. Results Exp-1 and Exp-2 had significantly higher fluoride release and recharge capabilities than CL and FL (P<.05). All tested sealants had similar MTBS before and after thermocycling. Exp-2 and Exp-1 had significantly lower microleakage scores (P<.05) than other groups. Conclusion The experimental sealants had higher fluoride release and recharge capabilities and similar or better retention than commercial sealants. PMID:23635887

  16. Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities

    Science.gov (United States)

    Din, Muhammad Imran; Arshad, Farhan; Hussain, Zaib; Mukhtar, Maria

    2017-12-01

    Copper nanoparticles (CuNPs) are of great interest due to their extraordinary properties such as high surface-to-volume ratio, high yield strength, ductility, hardness, flexibility, and rigidity. CuNPs show catalytic, antibacterial, antioxidant, and antifungal activities along with cytotoxicity and anticancer properties in many different applications. Many physical and chemical methods have been used to synthesize nanoparticles including laser ablation, microwave-assisted process, sol-gel, co-precipitation, pulsed wire discharge, vacuum vapor deposition, high-energy irradiation, lithography, mechanical milling, photochemical reduction, electrochemistry, electrospray synthesis, hydrothermal reaction, microemulsion, and chemical reduction. Phytosynthesis of nanoparticles has been suggested as a valuable alternative to physical and chemical methods due to low cytotoxicity, economic prospects, environment-friendly, enhanced biocompatibility, and high antioxidant and antimicrobial activities. The review explains characterization techniques, their main role, limitations, and sensitivity used in the preparation of CuNPs. An overview of techniques used in the synthesis of CuNPs, synthesis procedure, reaction parameters which affect the properties of synthesized CuNPs, and a screening analysis which is used to identify phytochemicals in different plants is presented from the recent published literature which has been reviewed and summarized. Hypothetical mechanisms of reduction of the copper ion by quercetin, stabilization of copper nanoparticles by santin, antimicrobial activity, and reduction of 4-nitrophenol with diagrammatic illustrations are given. The main purpose of this review was to summarize the data of plants used for the synthesis of CuNPs and open a new pathway for researchers to investigate those plants which have not been used in the past.

  17. Green Synthesis and Antibacterial Effect of Silver Nanoparticles Using Vitex Negundo L.

    Directory of Open Access Journals (Sweden)

    Fatima Abu Bakar

    2011-08-01

    Full Text Available Different biological methods are gaining recognition for the production of silver nanoparticles (Ag-NPs due to their multiple applications. One of the most important applications of Ag-NPs is their use as an anti-bacterial agent. The use of plants in the synthesis of nanoparticles emerges as a cost effective and eco-friendly approach. In this study the biosynthesis of silver nanoparticles using Vitex negundo L. extract and its antimicrobial properties has been reported. The resulting silver particles are characterized using transmission electron microscopy (TEM, X-ray diffraction (XRD and UV–Visible (UV-Vis spectroscopic techniques. The TEM study showed the formation of silver nanoparticles in the 10–30 nm range and average 18.2 nm in size. The XRD study showed that the particles are crystalline in nature, with a face centered cubic (fcc structure. The silver nanoparticles showed the antimicrobial activity against Gram positive and Gram negative bacteria. Vitex negundo L. was found to display strong potential for the synthesis of silver nanoparticles as antimicrobial agents by rapid reduction of silver ions (Ag+ to Ag0.

  18. Tyrosine-derived novel antimicrobial hydantoin polymers: synthesis and evaluation of anti-bacterial activities.

    Science.gov (United States)

    Ravichandran, Vasanthan; Rai, Rajani Kant; Kesavan, Venkitasamay; Jayakrishnan, A

    2017-12-01

    A new approach for the design and synthesis of cyclic N-halamine polymers having anti-bacterial activity based on a vinyl derivative of tyrosine-derived hydantoin is reported. The synthesis of N-halamine polymers generally involves the chemical modification of 5,5'-disubstituted hydantoin to introduce polymerizable vinyl moieties thereby restricting the halogen capture only on the amide nitrogen. Here we show the possibility of synthesizing vinyl monomers of N-halamine from α-amino acids wherein both the amide and imide nitrogens are available for halogen capture. Thus, a hydantoin monomer was synthesized from L-tyrosine and copolymerized with methyl methacrylate and 2-(hydroxyethyl)methacrylate, to obtain random co-polymers. The monomer and its co-polymers were characterized using NMR, IR, HRMS, GPC, DSC, EDAX and TGA analysis. Films of the co-polymers cast from 10% acetone solutions were exposed to sodium hypochlorite solution to activate the hydantoin moieties. The oxidative chlorine content of the films ranged from 0.6 to 0.9%. The activated films were exposed to both Gram positive (S. aureus) and Gram negative (E. coli) bacteria using standard protocols. Polymers having chlorine content as little as 0.6% exhibited 6 log reduction in the bacterial growth within 30 min of exposure. The method allows the halogenation of both amide and imide nitrogens and could be applied to the preparation of a number of vinyl hydantoins from many amino acids.

  19. Synthesis, characterization and properties

    Indian Academy of Sciences (India)

    Unknown

    C−H⋅⋅⋅O bonds leading to an intricate hydrogen bonding network. Keywords. Synthesis .... in the refinement riding on their respective parent atoms. ..... nent peaks at 326 and 255 nm which can be assigned to transitions of the intramolecularly hydrogen-bon- ded salicylidenimino chromophore. Cotton effects of negative ...

  20. Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer ...

    Indian Academy of Sciences (India)

    DOI 10.1007/s12039-015-0824-z. Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer activity of new Schiff base and its copper complex derived from sulfamethoxazole. I RAMA∗ and R SELVAMEENA. PG and Research Department of Chemistry, Seethalakshmi Ramaswami College,. Tiruchirappalli 620 002 ...

  1. Synthesis of 1-phenyl-3-(4'-nitrophenyl)-5-(3',4'-dimethoxy-6'-nitrophenyl)-2-pyrazoline and its antibacterial activity

    Science.gov (United States)

    Fauzi'ah, Lina; Wahyuningsih, Tutik Dwi

    2017-03-01

    Synthesis of pyrazoline substituted with nitro groups as antibacterial agent has been carried out by cycloaddition reaction. The compound was synthesized from chalcone and phenylhyrazine by refluxing them in 2-butanol for 24 h. The product was purified and characterized using FTIR and 1H-NMR spectrometers. The result showed that pyrazoline has been succesfully synthesized in 33.06% yield. The compund has antibacterial activity againts Bacillus subtilis and Shigella flexneri. However, it has tendency of activity for Gram-negative bacteria. In conclusion, the nitro groups that substituted in aromatic ring were predicted as a part of pharmacophore.

  2. Oxoperoxomolybdenum(VI complexes of catalytic and biomedical relevance: Synthesis, characterization, antibacterial activity and 3D-molecular modeling of some oxoperoxomolybdenum(VI chelates in mixed (O,O coordination environment involving maltol and β-diketoenolates

    Directory of Open Access Journals (Sweden)

    R.C. Maurya

    2016-09-01

    Full Text Available A new series of four mixed-ligand complexes of oxoperoxomolybdenum(VI of the general composition [MoO(O2(ma(L]·H2O, where maH = 3-hydroxy-2-methyl-4-pyrone and LH = o-acetoacetanisidide (o-aansH, o-acetoacetotoluidide (o-aatdH, acetylacetone (acacH or methylactoacetate (macacH, has been synthesized by the interaction of [MoO(O2]2+ (obtained in situ during the interaction of MoO3 and 30% H2O2 on continuous stirring at 50 °C for 24 h and the said ligands in aqueous-ethanol medium. The complexes so obtained have been characterized by elemental analyses, molybdenum determination, molar conductance, decomposition temperature and magnetic measurements, thermogravimetric studies, 1H NMR, IR, mass and electronic spectral studies. Antibacterial studies of one of the ligands maltol and the two representative metal complexes were also carried out and it has been observed that the complexes are more potent bactericides than the ligand. The 3D-molecular modeling and analysis for bond lengths and bond angles have also been carried out for one of the representative compound (1 to substantiate the proposed structure. Pseudopentagonal bipyramidal structures have been proposed for these complexes.

  3. Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus

    Directory of Open Access Journals (Sweden)

    Tej Singh

    2017-06-01

    Full Text Available In this study, biological synthesis of silver nanoparticles (AgNPs from supernatant of endophytic fungus Alternaria sp. isolated from the healthy leaves of Raphanus sativus is studied. The synthesized AgNPs are characterized using UV-vis spectroscopy and Fourier transform-infrared spectroscopy (FTIR. The structural analysis is done by powder X-ray diffraction (XRD method. The stability of AgNPs is studied by dynamic light scattering (DLS method. The size and shape of AgNPs are observed by transmission electron microscopy (TEM and atomic force microscopy (AFM and found to be spherical with an average particles size of 4–30 nm. Further, these AgNPs have been found to be highly toxic against human pathogenic bacteria, suggesting the possibility of using AgNPs as efficient antibacterial agents.

  4. Biosynthesis, characterization and antibacterial studies of silver nanoparticles using pods extract of Acacia auriculiformis

    Science.gov (United States)

    Nalawade, Pradnya; Mukherjee, Poulomi; Kapoor, Sudhir

    2014-08-01

    The present study reports an environmental friendly method for the synthesis of silver nanoparticles (Ag NPs) using an aqueous extract of Acacia auriculiformis that acts as reducing agent as well as capping agent. The obtained NPs were characterized by UV-vis absorption spectroscopy and showed a sharp surface plasmon absorption band at ∼400 nm. Fourier transform infrared spectroscopy (FTIR) showed nanoparticles were capped with plant compounds. Transmission electron microscopy (TEM) showed that the particles were spherical in nature with diameter ranging from 20 to 150 nm depending on the pH of the solution. The as-synthesized Ag NPs showed antibacterial activity against both Gram negative and Gram positive bacteria with more efficacy against Gram negative bacteria.

  5. Design, Synthesis, Molecular Docking, and Antibacterial Evaluation of Some Novel Flouroquinolone Derivatives as Potent Antibacterial Agent

    Directory of Open Access Journals (Sweden)

    Mehul M. Patel

    2014-01-01

    Full Text Available Objective. Quinolone moiety is an important class of nitrogen containing heterocycles widely used as key building blocks for medicinal agents. It exhibits a wide spectrum of pharmacophores and has bactericidal, antiviral, antimalarial, and anticancer activities. In view of the reported antimicrobial activity of various fluoroquinolones, the importance of the C-7 substituents is that they exhibit potent antimicrobial activities. Our objective was to synthesize newer quinolone analogues with increasing bulk at C-7 position of the main 6-fluoroquinolone scaffold to produce the target compounds which have potent antimicrobial activity. Methods. A novel series of 1-ethyl-6-fluoro-4-oxo-7-{4-[2-(4-substituted phenyl-2-(substituted-ethyl]-1-piperazinyl}-1,4-dihydroquinoline-3-carboxylic acid derivatives were synthesized. To understand the interaction of binding sites with bacterial protein receptor, the docking study was performed using topoisomerase II DNA gyrase enzymes (PDB ID: 2XCT by Schrodinger’s Maestro program. In vitro antibacterial activity of the synthesized compounds was studied and the MIC value was calculated by the broth dilution method. Results. Among all the synthesized compounds, some compounds showed potent antimicrobial activity. The compound 8g exhibited good antibacterial activity. Conclusion. This investigation identified the potent antibacterial agents against certain infections.

  6. Green Chemistry Approach for Efficient Synthesis of Schiff Bases of Isatin Derivatives and Evaluation of Their Antibacterial Activities

    Directory of Open Access Journals (Sweden)

    Jnyanaranjan Panda

    2013-01-01

    Full Text Available Microwave-assisted organic synthesis, a green chemistry approach, is nowadays widely used in the drug synthesis. Microwave-assisted synthesis improves both throughput and turnaround time for medicinal chemists by offering the benefits of drastically reduced reaction times, increased yields, and pure products. Schiff bases are the important class of organic compounds due to their flexibility, and structural diversities due to the presence of azomethine group which is helpful for elucidating the mechanism of transformation and rasemination reaction in biological system. This novel compound could also act as valuable ligands for the development of new chemical entities. In the present work, some Schiff bases of Isatin derivatives was synthesized using microwave heating method. Schiff base of Isatin were synthesized by condensation of the keto group of Isatin with different aromatic primary amines. They were characterized by means of spectral data and subsequently subjected to the in vitro antibacterial activities against gram positive and gram negative strains of microbes. It was observed that the compound with electron withdrawing substituents exhibited good antibacterial activities against almost all the micro organisms.

  7. Synthesis and Bioactivity Evaluation of Novel 2-Salicyloylbenzofurans as Antibacterial Agents

    Directory of Open Access Journals (Sweden)

    Phuong-Thuy T. Phan

    2017-04-01

    Full Text Available In order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three Gram-(+ strains (methicillin-sensitive Staphylococcus aureus (MSSA ATCC 29213, methicillin-resistant Staphylococcus aureus (MRSA ATCC 43300, and Streptococcus faecalis (S. faecalis ATCC 29212 and one Gram-(− strain (Escherichia coli (E. coli ATCC 25922. The 2-salicyloylbenzofuran heterocycles were generated by Rap–Stoermer condensation of salicylaldehydes with phenacyl bromides and then converted to diverse O-ether derivatives by Williamson synthesis. The targeted products were screened for in vitro qualitative (zone of inhibition and quantitative (MIC antibacterial activities by agar well diffusion assay and agar dilution method. Amongst the compounds, those bearing carboxylic acid functional group were found to exhibit reasonable activity against Gram-(+ bacterial strains including S. faecalis, MSSA and MRSA with the most potent antibacterial agent 8h (MICs = 0.06–0.12 mM. Besides, the 2-salicyloylbenzofurans partly displayed inhibitory activity against MRSA with the best MICs = 0.14 mM (8f and 0.12 mM (8h. Finally, the antibacterial results preliminarily suggested that the substituent bearing carboxylic acid group at salicyloyl-C2 and the bromine atoms on the benzofuran moiety seem to be the functionality necessary for antibacterial activities.

  8. Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Emblica officinalis fruit extract

    Science.gov (United States)

    Ramesh, P. S.; Kokila, T.; Geetha, D.

    2015-05-01

    A green straight forward method of synthesizing silver nanoparticles (AgNPs) in an aqueous medium was designed using Emblica officinalis (EO) fruit extract as stabilizer and reducer. The formation of AgNPs depends on the effect of extract concentration and pH were studied. The AgNPs was synthesized using E.officinalis (fruit extract) and nanoparticles were characterized using UV-Vis spectrophotometer, the presence of biomolecules of E.officinalis capped in AgNPs was found by FT-IR analysis, shape and size were examined by SEM and XRD. The XRD analysis respects the Bragg's law and confirmed the crystalline nature of silver nanoparticles. From XRD the average size of AgNPs was found to be around 15 nm. AFM has proved to be very helpful in the determination and verification of various morphological features and parameters. EO fruit extract mediated AgNPs was synthesized and confirmed through kinetic behavior of nanoparticles. The shape of the bio-synthesized AgNPs was spherical. Potent biomolecules of E.officinalis such as polyphenols, glucose, and fructose was capped with AgNPs which reduces the toxicity. The synthesized AgNPs were tested for its antibacterial activity against the isolates by disc diffusion method. The obtained results confirmed that the E.officinalis fruit extract is a very good bioreductant for the synthesis of AgNPs. It was investigated that the synthesized AgNPs showed inhibition and had significant antibacterial against both gram-positive and gram-negative bacterial strains.

  9. Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential

    Directory of Open Access Journals (Sweden)

    Patra JK

    2015-12-01

    Full Text Available Jayanta Kumar Patra, Kwang-Hyun Baek School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, Republic of Korea Abstract: Biological synthesis of nanoparticles using nontoxic, eco-friendly approaches is gaining importance owing to their fascinating biocompatibility and environmentally benign nature. This study describes the green synthesis approach for synthesis of gold nanoparticles (ANPs using aqueous extract of the rind of watermelon as a fruit waste and evaluate its biopotential in terms of proteasome inhibitory activity, antibacterial, and antioxidant potential. The synthesized ANPs were characterized using UV–vis spectroscopy, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The surface plasmon resonance spectra of ANPs were obtained at 560 nm. Scanning electron microscopy image revealed that particles had a spherical shape and have a size distribution of 20–140 nm, followed by the elemental analysis by energy-dispersive X-ray spectroscopy. X-ray diffraction analysis confirmed the crystallite nature of the ANPs and Fourier-transform infrared spectroscopy revealed the involvement of bioactive compounds from watermelon rind in the synthesis, capping, and stabilization of ANPs. ANPs exhibited potential antibacterial activity against five different foodborne pathogenic bacteria with diameter of inhibition zones ranged between 9.23 and 11.58 mm. They also displayed strong synergistic antibacterial activity together with kanamycin (11.93–21.08 mm inhibition zones and rifampicin (10.32–24.84 mm inhibition zones. ANPs displayed strong antioxidant activity in terms of DPPH radical scavenging (24.69%, nitric oxide scavenging (25.62%, ABTS scavenging (29.42%, and reducing power. Significantly high proteasome inhibitory potential of the ANPs (28.16% could be highly useful for cancer treatment and targeted cancer drug delivery. Overall, results highlight a

  10. Hydrothermal synthesis, characterization and luminescent ...

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 39; Issue 4. Hydrothermal synthesis, characterization and luminescent properties of lanthanide-doped NaLaF 4 nanoparticles. JIGMET LADOL HEENA KHAJURIA SONIKA KHAJURIA ... Keywords. Citric acid; X-ray diffraction; down-conversion emission; energy transfer.

  11. Hydrothermal synthesis, characterization and luminescent ...

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 39; Issue 4. Hydrothermal synthesis, characterization and luminescent properties of lanthanide-doped NaLaF 4 nanoparticles. JIGMET LADOL HEENA KHAJURIA SONIKA KHAJURIA HAQ NAWAZ SHEIKH. Volume 39 Issue 4 August 2016 pp 943-952 ...

  12. Green synthesis of silver nanoparticles from seed extract of Brassica nigra and its antibacterial activity

    Directory of Open Access Journals (Sweden)

    RAKSHA PANDIT

    2015-05-01

    Full Text Available Pandit R. 2015. Green synthesis of silver nanoparticles from seed extract of Brassica nigra and its antibacterial activity. Nusantara Bioscience 7: 15-19. We report the green synthesis of silver nanoparticles using seed extract of Brassica nigra. UV-visible spectroscopic analysis showed the absorbance peak at 432 nm which indicated the synthesis of silver nanoparticles. Nanoparticles Tracking and Analysis (NTA was used to determine the size of synthesized silver nanoparticles. Zeta potential analysis was carried out to study the stability of nanoparticles while FTIR analysis confirmed the presence of proteins as capping agents that provided stability to nanoparticles in colloid. Antibacterial activity of silver nanoparticles was evaluated against Propionibacterium acnes, Pseudomonas aeruginosa and Klebsiella pneumoniae. The activity of Vancomycin was significantly increased in combination with silver nanoparticles showing synergistic activity against all bacteria while the maximum activity was noted against P. acnes.

  13. Characterization and antibacterial properties of stable silver substituted hydroxyapatite nanoparticles synthesized through surfactant assisted microwave process

    Energy Technology Data Exchange (ETDEWEB)

    Iqbal, Nida [Medical Implant Technology Group (MEDITEG), Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia); Abdul Kadir, Mohammed Rafiq, E-mail: rafiq@biomedical.utm.my [Medical Implant Technology Group (MEDITEG), Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia); Nik Malek, Nik Ahmad Nazim [Faculty of Bioscience and Medical Engineering (FBME), Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia); Mahmood, Nasrul Humaimi Bin [Medical Implant Technology Group (MEDITEG), Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia); Murali, Malliga Raman; Kamarul, T. [Tissue Engineering Group, NOCERAL, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)

    2013-09-01

    Highlights: • Stable nano sized silver substitute hydroxyapatite is prepared under surfactant assisted microwave process at 600 W power for 7 min. • The nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. • Increase in silver concentration resulted in better dielectric properties. • Good antibacterial activity and silver release. - Abstract: The present study reports a relatively simple method for the synthesis of stable silver substituted hydroxyapatite nanoparticles with controlled morphology and particle size. In order to achieve this, CTAB is included as a surfactant in the microwave refluxing process (600 W for 7 min). The nanoparticles produced with different silver ion concentrations (0.05, 0.1 and 0.2 wt%) were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (FESEM), energy dispersive X-ray (EDX) and Brunauer–Emmett–Teller (BET) analysis. XRD and FTIR analyses reveal that the Ag-HA nanoparticles were phase pure at 1000 °C. FESEM images showed that the produced nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. The dielectric properties suggest that the increase in dielectric constant (ε′) and dissipation factor (D) values with increasing Ag concentrations. Antibacterial performance of the Ag-HA samples elucidated using disk diffusion technique (DDT) and minimum inhibitory concentration (MIC) demonstrates anti-bacterial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. This effect was dose dependent and was more pronounced against Gram-negative bacteria than Gram-positive organisms.

  14. Characterization and antibacterial properties of stable silver substituted hydroxyapatite nanoparticles synthesized through surfactant assisted microwave process

    International Nuclear Information System (INIS)

    Iqbal, Nida; Abdul Kadir, Mohammed Rafiq; Nik Malek, Nik Ahmad Nazim; Mahmood, Nasrul Humaimi Bin; Murali, Malliga Raman; Kamarul, T.

    2013-01-01

    Highlights: • Stable nano sized silver substitute hydroxyapatite is prepared under surfactant assisted microwave process at 600 W power for 7 min. • The nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. • Increase in silver concentration resulted in better dielectric properties. • Good antibacterial activity and silver release. - Abstract: The present study reports a relatively simple method for the synthesis of stable silver substituted hydroxyapatite nanoparticles with controlled morphology and particle size. In order to achieve this, CTAB is included as a surfactant in the microwave refluxing process (600 W for 7 min). The nanoparticles produced with different silver ion concentrations (0.05, 0.1 and 0.2 wt%) were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (FESEM), energy dispersive X-ray (EDX) and Brunauer–Emmett–Teller (BET) analysis. XRD and FTIR analyses reveal that the Ag-HA nanoparticles were phase pure at 1000 °C. FESEM images showed that the produced nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. The dielectric properties suggest that the increase in dielectric constant (ε′) and dissipation factor (D) values with increasing Ag concentrations. Antibacterial performance of the Ag-HA samples elucidated using disk diffusion technique (DDT) and minimum inhibitory concentration (MIC) demonstrates anti-bacterial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. This effect was dose dependent and was more pronounced against Gram-negative bacteria than Gram-positive organisms

  15. Synthesis of photothermal nanocomposites and their application to antibacterial assays

    Science.gov (United States)

    Yang, Ning; Wang, Chun; Wang, Xiaoyu; Li, Lidong

    2018-04-01

    In this work, we report a novel gold nanorod (AuNR)-based nanocomposite that shows strong binding to bacterium and high antibacterial efficiency. The AuNRs were used as a photothermal material to transform near-infrared radiation (NIR) into heat. We selected poly (acrylic acid) to modify the surface of the AuNRs based on a simple self-assembly method. After conjugation of the bacterium-binding molecule vancomycin, the nanocomposites were capable of efficiently gathering on the cell walls of bacteria. The nanocomposites exhibited a high bacterial inhibition capability owing to NIR-induced heat generation in situ. Therefore, the prepared photothermal nanocomposites show great potential for use in antibacterial assays.

  16. Synthesis and antibacterial activity of sulfonamides. SAR and DFT studies

    Science.gov (United States)

    Boufas, Wahida; Dupont, Nathalie; Berredjem, Malika; Berrezag, Kamel; Becheker, Imène; Berredjem, Hajira; Aouf, Nour-Eddine

    2014-09-01

    A series of substituted sulfonamide derivatives were synthesized from chlorosulfonyl isocyanate (CSI) in tree steps (carbamoylation, sulfamoylation and deprotection). Antibacterial activity in vitro of some newly formed compounds investigated against clinical strains Gram-positive and Gram-negative: Escherichia coli and Staphylococcus aureus applying the method of dilution and minimal inhibition concentration (MIC) methods. These compounds have significant bacteriostatic activity with totalities of bacterial strains used. DFT calculations with B3LYP/6-31G(d) level have been used to analyze the electronic and geometric characteristics deduced for the stable structure of three compounds presenting conjugation between a nitrogen atom N through its lone pair and an aromatic ring next to it. The principal quantum chemical descriptors have been correlated with the antibacterial activity.

  17. Synthesis and antibacterial studies of some novel isoxazoline derivatives

    Directory of Open Access Journals (Sweden)

    TEJASKUMAR SHAH

    2007-05-01

    Full Text Available A series of 3-[3-(2,4-dichloro-5-fluorophenyl-5-(2-furyl-4,5-dihydro-1H-pyrazol-1-yl]-5-(substituted phenyl/2-thienylisoxazolines (4a-j were prepared. The structures of the isoxazoline derivatives were confirmed on the bases of elemental analysis and spectral data. The compounds were screened for their in vitro antibacterial activity using gram-positive bacteria and gram-negative bacteria.

  18. Synthesis and Antibacterial Screening of Novel Derivatives of Embelin

    Science.gov (United States)

    Subramanian, L.; Leema, M.; Pradeep, N. S.; Joy, B.; Pillai, Z. S.

    2018-02-01

    Embelin, a naturally occurring compound extracted from Embelia ribes is used in Ayurvedic system of medicine owing to its wide spectrum of biological activities. In the present work, we have aimed at improving the efficacy of Embelin by appropriate structural modifications. A few novel derivatives of Embelin have been prepared. The antibacterial screening of these derivatives were carried out and compared with a well known antibiotic, Streptomycin. The derivatives exhibited better activity than Streptomycin and the lead molecule, Embelin.

  19. SHORT COMMUNICATION SYNTHESIS, CHARACTERIZATION ...

    African Journals Online (AJOL)

    Preferred Customer

    oxazine-. 2-ol methyl-2,3-diphenyl-2H-1,4-oxazine-2-ol (2). Characterization was performed using elemental analysis, UV-Vis and 1HNMR spectroscopy. The structure of this oxazine compound was determined by X-ray crystallography, and we ...

  20. Synthesis and antibacterial evaluation of novel cationic chalcone derivatives possessing broad spectrum antibacterial activity.

    Science.gov (United States)

    Chu, Wen-Chao; Bai, Peng-Yan; Yang, Zhao-Qing; Cui, De-Yun; Hua, Yong-Gang; Yang, Yi; Yang, Qian-Qian; Zhang, En; Qin, Shangshang

    2018-01-01

    There is an urgent need to identify new antibiotics with novel mechanisms that combat antibiotic resistant bacteria. Herein, a series of chalcone derivatives that mimic the essential properties of cationic antimicrobial peptides were designed and synthesized. Antibacterial activities against drug-sensitive bacteria, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Salmonella enterica, as well as clinical multiple drug resistant isolates of methicillin-resistant S. aureus (MRSA), KPC-2-producing and NDM-1-producing Carbapenem-resistant Enterobacteriaceae were evaluated. Representative compounds 5a (MIC: 1 μg/mL against S. aureus, 0.5 μg/mL against MRSA) and 5g (MIC: 0.5 μg/mL against S. aureus, 0.25 μg/mL against MRSA) showed good bactericidal activity against both Gram-positive and Gram-negative bacteria, including the drug-resistant species MRSA, KPC and NDM. These membrane-active antibacterial compounds were demonstrated to reduce the viable cell counts in bacterial biofilms effectively and do not induce the development of resistance in bacteria. Additionally, these representative molecules exhibited negligible toxicity toward mammalian cells at a suitable concentration. The combined results indicate that this series of cationic chalcone derivatives have potential therapeutic effects against bacterial infections. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  1. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

    Directory of Open Access Journals (Sweden)

    Patcharaporn Tippayawat

    2016-10-01

    Full Text Available Background There is worldwide interest in silver nanoparticles (AgNPs synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV with antibacterial activity. Methods AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively

  2. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity.

    Science.gov (United States)

    Tippayawat, Patcharaporn; Phromviyo, Nutthakritta; Boueroy, Parichart; Chompoosor, Apiwat

    2016-01-01

    There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa . The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 o C for 6 h and 200 o C for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in pharmaceutical, biotechnological

  3. Rapid biological synthesis of silver nanoparticles using Leucas martinicensis leaf extract for catalytic and antibacterial activity.

    Science.gov (United States)

    Ashokkumar, S; Ravi, S; Kathiravan, V; Velmurugan, S

    2014-10-01

    A novel green approach for the synthesis and stabilization of silver nanoparticles (AgNPs) using water extract of Leucas martinicensis leaf has been developed. As obtained, the nanoparticles are characterized by UV-visible (UV-Vis), transmission electron microscope (TEM), and X-ray diffraction (XRD). The crystalline nature of the AgNPs is confirmed by the prominent peaks in the XRD pattern. FTIR spectra suggest that the possible biomolecules are responsible for the efficient stabilization of the sample. The effects of leaf quantity on the biosynthesis of AgNPs are investigated by UV-Vis spectrophotometer. The synthesized AgNPs are observed to have a good catalytic activity on the reduction of methylene blue by L. martinicensis leaf. This is confirmed by the decrease in absorbance maximum values of methylene blue with respect to time through UV-Vis spectrophotometer. Moreover, the antibacterial activity of synthesized AgNPs against Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Escherichia coli are screened.

  4. Green synthesis of silver nanoparticles by Escherichia coli : Analysis of antibacterial activity

    Directory of Open Access Journals (Sweden)

    Koilparambil Divya

    2016-07-01

    Full Text Available The emerging infectious diseases and the development of drug resistance in the pathogenic microorganism is a matter of serious concern. Despite the increased knowledge of microbial pathogenesis and application of modern therapeutics, the morbidity and mortality associated with the microbial infections still remains high. Therefore, there is a pressing demand to discover novel strategies and identify new antimicrobial agents to develop the next generation of drugs or agents to control microbial infections. The use of nanoparticles is gaining impetus in the present century as they possess defined chemical, optical and mechanical properties. In the present study green synthesis of silver nanoparticles by Escherichia coli has been done. Various parameters such as mixing ratio of culture supernatant and silver nitrate, media, temperature and pH for production of silver nanoparticles were optimised. The nanoparticles synthesised was characterized using SEM, FTIR and XRD. The antibacterial activity of silver nanoparticles synthesised using both pellet and supernatant against human pathogens Salmonella typhi, Vibrio cholerae, Bacillus subtilis and Klebsiella pneumoniae was analysed and MIC was calculated as 20µg and 50µg respectively.

  5. Facile and green synthesis of phytochemicals capped platinum nanoparticles and in vitro their superior antibacterial activity.

    Science.gov (United States)

    Tahir, Kamran; Nazir, Sadia; Ahmad, Aftab; Li, Baoshan; Khan, Arif Ullah; Khan, Zia Ul Haq; Khan, Faheem Ullah; Khan, Qudrat Ullah; Khan, Abrar; Rahman, Aziz Ur

    2017-01-01

    The increase in the severe infectious diseases and resistance of the majority of the bacterial pathogens to the available drug is a serious problem now a day. In order to overcome this problem it is necessary to develop new therapeutic agents which are non-toxic and more effective to inhibit these microbial pathogens. For this purpose the plant extract of highly active medicinal plant, Taraxacum laevigatum was used for the synthesis of platinum nanoparticles (PtNPs) to enhance its bio-activities. The surface plasmon resonance peak appeared at 283nm clearly represent the formation of PtNPs. The results illustrate that the bio-synthesized PtNPs were uniformly dispersed, small sized (2-7nm) and spherical in shape. The green synthesized PtNPs were characterized by UV-vis spectroscopy, XRD, TEM, SEM, EDX, DLS and FTIR. These nanoparticles were tested against gram positive bacteria (Bacillus subtilis) and gram negative bacteria (Pseudomonas aeruginosa). The bio-synthesized PtNPs were examined to be more effective against both of the bacteria. The results showed, that the zone of inhibition of PtNPs against P. aeruginosa was 15 (±0.5) mm and B. subtilis was 18 (±0.8) mm. The most significant outcome of this examination is that PtNPs exhibited strong antibacterial activity against P. aeruginosa and B. subtilis which have strong defensive system against several antibiotics. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Synthesis, Antibacterial and Thermal Studies of Cellulose Nanocrystal Stabilized ZnO-Ag Heterostructure Nanoparticles

    Directory of Open Access Journals (Sweden)

    Mohd Zobir Hussein

    2013-05-01

    Full Text Available Synthesis of ZnO-Ag heterostructure nanoparticles was carried out by a precipitation method with cellulose nanocrystals (CNCs as a stabilizer for antimicrobial and thermal studies. ZnO-Ag nanoparticles were obtained from various weight percentages of added AgNO3 relative to Zn precursors for evaluating the best composition with enhanced functional properties. The ZnO-Ag/CNCs samples were characterized systematically by TEM, XRD, UV, TGA and DTG. From the TEM studies we observed that ZnO-Ag heterostructure nanoparticles have spherical shapes with size diameters in a 9–35 nm range. The antibacterial activities of samples were assessed against the bacterial species Salmonella choleraesuis and Staphylococcus aureus. The CNC-stabilized ZnO-Ag exhibited greater bactericidal activity compared to cellulose-free ZnO-Ag heterostructure nanoparticles of the same particle size. The incorporation of ZnO-Ag hetreostructure nanoparticles significantly increased the thermal stability of cellulose nanocrystals.

  7. Synthesis of magnetic graphene oxide–TiO2 and their antibacterial properties under solar irradiation

    International Nuclear Information System (INIS)

    Chang, Ying-Na; Ou, Xiao-Ming; Zeng, Guang-Ming; Gong, Ji-Lai; Deng, Can-Hui; Jiang, Yan; Liang, Jie; Yuan, Gang-Qiang; Liu, Hong-Yu; He, Xun

    2015-01-01

    Highlights: • Magnetic graphene oxide–TiO 2 (MGO–TiO 2 ) composites were synthesized. • MGO–TiO 2 had excellent antibacterial activity toward Escherichia coli. • MGO–TiO 2 could effectively and rapidly separate from aqueous solution. • Carbonates and phosphates significantly reduced the bacterial survival rate. - Abstract: Titanium dioxide (TiO 2 ) has been intensively researched and increasingly used as antibacterial agent, but it suffers from separation inconvenience. Its effective removal from water after reaction while maintaining its high antibacterial activity becomes necessary. In this work, it was the first time the magnetic graphene oxide–TiO 2 (MGO–TiO 2 ) composites were prepared through a simple synthesis method. The results indicated that MGO–TiO 2 exhibited a good antibacterial activity against Escherichia coli. MGO–TiO 2 was found to almost completely inactivate the E. coli within 30 min under solar irradiation. The effect of inorganic ions present in E. coli suspension was also evaluated. Compared with other ions, HCO 3 − and HPO 4 2− had a greater influence on the antibacterial property

  8. Purification and characterization of an antibacterial factor from snail mucus.

    Science.gov (United States)

    Kubota, Y; Watanabe, Y; Otsuka, H; Tamiya, T; Tsuchiya, T; Matsumoto, J J

    1985-01-01

    The antibacterial factor from the body surface of the African giant snail, Achatina fulica Férussac, was isolated by DEAE-Toyopearl 650M ion exchange chromatography. The isolated preparation exhibited highly positive antibacterial activity both for the Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus and for the Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but it lost such activity when heated at 75 degrees C for 5 min. The antibacterial factor of the snail mucus was a glycoprotein whose molecular weight (MW) was about 160,000. It was composed of two subunits of MW 70,000-80,000.

  9. Zinc oxide nanoparticle-coated films: fabrication, characterization, and antibacterial properties

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Yunhong, E-mail: y.jiang@leeds.ac.uk [University of Leeds, Institute of Particle Science and Engineering (United Kingdom); O’Neill, Alex J. [University of Leeds, School of Molecular and Cellular Biology (United Kingdom); Ding, Yulong [University of Leeds, Institute of Particle Science and Engineering (United Kingdom)

    2015-04-15

    In this article, novel antibacterial PVC-based films coated with ZnO nanoparticles (NPs) were fabricated, characterized, and studied for their antibacterial properties. It was shown that the ZnO NPs were coated on the surface of the PVC films uniformly and that the coating process did not affect the size and shape of the NPs on the surface of PVC films. Films coated with concentrations of either 0.2 or 0.075 g/L of ZnO NPs exhibited antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, but exhibited no antifungal activity against Aspergillus flavus and Penicillium citrinum. Smaller particles (100 nm) exhibited more potent antibacterial activity than larger particles (1000 nm). All ZnO-coated films maintained antibacterial activity after 30 days in water.

  10. Synthesis, Spectral, and In Vitro Antibacterial Studies of Organosilicon(IV) Complexes with Schiff Bases Derived from Amino Acids.

    Science.gov (United States)

    Singh, Har Lal; Singh, Jangbhadur; Mukherjee, A

    2013-01-01

    The present work stems from our interest in the synthesis, characterization, and antibacterial evaluation of organosilicon(IV) complexes of a class of amino-acid-based Schiff base which have been prepared by the interaction of ethoxytrimethylsilane with the Schiff bases (N OH) in 1 : 1 molar ratio. These complexes have been characterized by elemental analysis, molar conductance, and spectroscopic studies including electronic IR and NMR ((1)H, (13)C, and (29)Si) spectroscopy. The analytical and spectral data suggest trigonal bipyramidal geometry around the silicon atom in the resulting complexes. The ligands and their organosilicon complexes have also been evaluated for in vitro antimicrobial activity against bacteria (Bacillus cereus, Nocardia spp., E. aerogenes, Escherichia coli, Klebsiella spp., and Staphylococcus spp.). The complexes were found to be more potent as compared to the ligands.

  11. Easy Access to Evans’ Oxazolidinones. Stereoselective Synthesis and Antibacterial Activity of a New 2-Oxazolidinone Derivative

    Directory of Open Access Journals (Sweden)

    Gaspar Diaz

    2014-06-01

    Full Text Available An interesting new approach was developed for the synthesis of Evans’ chiral auxiliaries with excellent yields. In turn, another new stereoselective and efficient strategy has also allowed for the preparation of a 2-oxazolidinone derivative in 34% overall yield from the Morita-Baylis-Hillman adduct. The antibacterial activity of this oxazolidinone was tested against Staphylococcus aureus strains isolated from animals with mastitis infections.

  12. Silver Nanoparticles Mediated by Costus afer Leaf Extract: Synthesis, Antibacterial, Antioxidant and Electrochemical Properties.

    Science.gov (United States)

    Elemike, Elias E; Fayemi, Omolola E; Ekennia, Anthony C; Onwudiwe, Damian C; Ebenso, Eno E

    2017-04-29

    Synthesis of metallic and semiconductor nanoparticles through physical and chemical routes has been extensively reported. However, green synthesized metal nanoparticles are currently in the limelight due to the simplicity, cost-effectiveness and eco-friendliness of their synthesis. This study explored the use of aqueous leaf extract of Costus afer in the synthesis of silver nanoparticles (CA-AgNPs). The optical and structural properties of the resulting silver nanoparticles were studied using UV-visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infra-red spectrophotometer (FTIR). TEM images of the silver nanoparticles confirmed the existence of monodispersed spherical nanoparticles with a mean size of 20 nm. The FTIR spectra affirmed the presence of phytochemicals from the Costus afer leaf extract on the surface of the silver nanoparticles. The electrochemical characterization of a CA-AgNPs/multiwalled carbon nanotubes (MWCNT)-modified electrode was carried out to confirm the charge transfer properties of the nanocomposites. The comparative study showed that the CA-AgNPs/MWCNT-modified electrode demonstrated faster charge transport behaviour. The anodic current density of the electrodes in Fe(CN)₆] 4- /[Fe(CN)₆] 3- redox probe follows the order: GCE/CA-Ag/MWCNT (550 mA/cm²) > GCE/MWCNT (270 mA/cm²) > GCE (80 mA/cm²) > GCE/CA-Ag (7.93 mA/cm²). The silver nanoparticles were evaluated for their antibacterial properties against Gram negative ( Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa ) and Gram positive ( Bacillus subtilis and Staphylococcus aureus ) pathogens. The nanoparticles exhibited better inhibition of the bacterial strains compared to the precursors (leaf extract of Costus afer and silver nitrate). Furthermore, the ability of the nanoparticles to scavenge DPPH radicals at different concentrations was studied using the DPPH radical scavenging assay and compared to

  13. Silver Nanoparticles Mediated by Costus afer Leaf Extract: Synthesis, Antibacterial, Antioxidant and Electrochemical Properties

    Directory of Open Access Journals (Sweden)

    Elias E. Elemike

    2017-04-01

    Full Text Available Synthesis of metallic and semiconductor nanoparticles through physical and chemical routes has been extensively reported. However, green synthesized metal nanoparticles are currently in the limelight due to the simplicity, cost-effectiveness and eco-friendliness of their synthesis. This study explored the use of aqueous leaf extract of Costus afer in the synthesis of silver nanoparticles (CA-AgNPs. The optical and structural properties of the resulting silver nanoparticles were studied using UV-visible spectroscopy, scanning electron microscopy (SEM, transmission electron microscopy (TEM and Fourier transform infra–red spectrophotometer (FTIR. TEM images of the silver nanoparticles confirmed the existence of monodispersed spherical nanoparticles with a mean size of 20 nm. The FTIR spectra affirmed the presence of phytochemicals from the Costus afer leaf extract on the surface of the silver nanoparticles. The electrochemical characterization of a CA-AgNPs/multiwalled carbon nanotubes (MWCNT-modified electrode was carried out to confirm the charge transfer properties of the nanocomposites. The comparative study showed that the CA-AgNPs/MWCNT-modified electrode demonstrated faster charge transport behaviour. The anodic current density of the electrodes in Fe(CN6]4−/[Fe(CN6]3− redox probe follows the order: GCE/CA-Ag/MWCNT (550 mA/cm2 > GCE/MWCNT (270 mA/cm2 > GCE (80 mA/cm2 > GCE/CA-Ag (7.93 mA/cm2. The silver nanoparticles were evaluated for their antibacterial properties against Gram negative (Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Gram positive (Bacillus subtilis and Staphylococcus aureus pathogens. The nanoparticles exhibited better inhibition of the bacterial strains compared to the precursors (leaf extract of Costus afer and silver nitrate. Furthermore, the ability of the nanoparticles to scavenge DPPH radicals at different concentrations was studied using the DPPH radical scavenging assay and compared to

  14. STUDY ON THE SYNTHESIS OF MONOLAURIN AS ANTIBACTERIAL AGENT AGAINTS Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Galuh Widiyarti

    2010-06-01

    Full Text Available The monolaurin compound had been synthesized from lauric acid and glycerol by using sulfuric acid (H2SO4 as catalyst. The synthesis of monolaurin was done by batch esterification on the free solvent system. The esterification reaction was performed on the equivalent mol ratio between lauric acid and glycerol 1:1, in the presence of 5% H2SO4, at 130 °C, for 6 hours, produced ester compounds on 59.29%. The products of column chromatography on silica gel purification are monolaurin and dilaurin in amount of 31.05 and 4.48%, respectively. The monolaurin and dilaurin were identified by TLC, FTIR, GC-MS, and NMR spectrometer. The spectral data of monolaurin was compared to spectral data of standard monolaurin. The result of NMR identifications showed that synthesis products were a-monolaurin and a,a'-dilaurin. The antibacterial activity of synthesis products was tested against Staphylococcus aureus. The activity result showed that the antibacterial activity of monolaurin is more active than dilaurin.   Keywords: monolaurin, esterification, identification, antibacterial activity.

  15. Characterization and antibacterial properties of porous fibers containing silver ions

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Zhaoyang; Fan, Chenxu; Tang, Xiaopeng; Zhao, Jianghui; Song, Yanhua; Shao, Zhongbiao [National Engineering Laboratory for Modern Silk, College of Textile and Engineering, Soochow University, 199 Ren-ai Road, Suzhou 215123 (China); Xu, Lan, E-mail: lanxu@suda.edu.cn [National Engineering Laboratory for Modern Silk, College of Textile and Engineering, Soochow University, 199 Ren-ai Road, Suzhou 215123 (China); Nantong Textile Institute of Soochow University, 58 Chong-chuan Road, Nantong 226018 (China)

    2016-11-30

    Highlights: • Antibacterial electrospun PLA porous fibers containing silver ions were prepared. • Porous structure and porosity of PLA/Ag{sup +} porous fibers were investigated. • The antibacterial effects of PLA/Ag{sup +} porous fibers were studied. • The released mechanism of silver ions in the porous fibers was illustrated. • The porous structure could improve the antibacterial properties. - Abstract: Materials prepared on the base of bioactive silver compounds have become more and more popular. In the present work, the surface morphology, structure and properties, of electrospun Polylactide Polylactic acid (PLA) porous fibers containing various ratios of silver ions were investigated by a combination of X-ray photoelectron spectroscopy (XPS), universal testing machine, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and et al. The biological activities of the proposed porous fibers were discussed in view of the released silver ions concentration. Antibacterial properties of these porous fibers were studied using two bacterial strains: Escherichia coli (E. coli) and Methicillin-resistant Staphylococcus aureus (MRSA). Results of the antibacterial testing suggested that PLA porous fibers containing silver ions could be used as potent antibacterial wound dressing materials in the biomedical field.

  16. Characterization and antibacterial properties of porous fibers containing silver ions

    International Nuclear Information System (INIS)

    Sun, Zhaoyang; Fan, Chenxu; Tang, Xiaopeng; Zhao, Jianghui; Song, Yanhua; Shao, Zhongbiao; Xu, Lan

    2016-01-01

    Highlights: • Antibacterial electrospun PLA porous fibers containing silver ions were prepared. • Porous structure and porosity of PLA/Ag + porous fibers were investigated. • The antibacterial effects of PLA/Ag + porous fibers were studied. • The released mechanism of silver ions in the porous fibers was illustrated. • The porous structure could improve the antibacterial properties. - Abstract: Materials prepared on the base of bioactive silver compounds have become more and more popular. In the present work, the surface morphology, structure and properties, of electrospun Polylactide Polylactic acid (PLA) porous fibers containing various ratios of silver ions were investigated by a combination of X-ray photoelectron spectroscopy (XPS), universal testing machine, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and et al. The biological activities of the proposed porous fibers were discussed in view of the released silver ions concentration. Antibacterial properties of these porous fibers were studied using two bacterial strains: Escherichia coli (E. coli) and Methicillin-resistant Staphylococcus aureus (MRSA). Results of the antibacterial testing suggested that PLA porous fibers containing silver ions could be used as potent antibacterial wound dressing materials in the biomedical field.

  17. Highly antioxidant carotene-lipid nanocarriers: synthesis and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Lacatusu, Ioana; Badea, Nicoleta, E-mail: nicoleta.badea@gmail.com; Ovidiu, Oprea [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania); Bojin, Dionezie [Faculty of Engineering and Materials Science (Romania); Meghea, Aurelia [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania)

    2012-06-15

    The objective of this study was to explore the potential of two natural oils (squalene-Sq and grape seed oil-GSO) to prepare biocompatible antioxidant nanostructured lipid carriers-NLCs as a safety and protective formulation for sensitive {beta}-carotene. For this purpose different oil-in-water nanoemulsions stabilized by a combination of alkylpolyoxy ethylene sorbitans, lecithin and a block copolymer, were prepared using a melt high-shear homogenization process. The physico-chemical characteristics of the carotene-loaded NLCs were firstly investigated in detail. The smaller lipid nanoparticles have been obtained by using Tween 20 as main non-ionic surfactant, with average diameters of about 85 nm for GSO and 89 nm for Sq, with a polydispersity index <0.19. The developed carotene-NLCs presented an excellent physical stability with almost all zeta potential values ranging between -29 Division-Sign -40 mV. The differential scanning calorimetry analysis showed that the {beta}-carotene incorporation has led to a perturbation of solid lipid matrix with a less ordered arrangement. By UV-Vis spectroscopy it was evidenced that after encapsulation {beta}-carotene adopts a supramolecular structure demonstrated by appearance of a shoulder at 530 nm related to a {beta}-carotene triplet-triplet absorption. The carotene-NLCs have been also evaluated in terms of in vitro antioxidant properties. The presence of Sq and GSO produced a significant effect on the antioxidant capacity of developed NLCs. The samples prepared with GSO and Tween 80 as main surfactant showed the highest antioxidant activity (AA %) against free oxygen radicals, exhibiting an enhancement of 35 % for loaded NLCs, as comparing to pure carotene. In addition to these properties, the ability of NLCs to manifest antibacterial activity was tested against Escherichiacoli bacteria. The antibacterial analysis shown that loaded-NLCs develop an effective inhibition zone against bacteria growth and it was dependent in a

  18. A new trinuclear Zn(II) complex {[Zn(μ-ONN)(μ.sub.2./sub.-O)(μ-OO)].sub.2./sub.Zn}: Synthesis, characterization, thermal decomposition and antibacterial activity

    Czech Academy of Sciences Publication Activity Database

    Far, B.S.; Grivani, G.; Khalaji, A.D.; Khorshidi, M.; Eigner, Václav; Dušek, Michal

    2017-01-01

    Roč. 28, č. 6 (2017), s. 3217-3227 ISSN 1040-7278 R&D Projects: GA ČR(CZ) GA14-03276S Institutional support: RVO:68378271 Keywords : trinuclear complex * Schiff base * single-crystal structure analysis * ZnO nano particle * antibacterial activity Subject RIV: BM - Solid Matter Physics ; Magnetism OBOR OECD: Condensed matter physics (including formerly solid state physics, supercond.) Impact factor: 1.471, year: 2016

  19. Synthesis of Silver Polymer Nanocomposites and Their Antibacterial Activity

    Science.gov (United States)

    Gavade, Chaitali; Shah, Sunil; Singh, N. L.

    2011-07-01

    PVA (Polyvinyl Alcohol) silver nanocomposites of different sizes were prepared by chemical reduction method. Silver nitrate was taken as the metal precursor and amine hydrazine as a reducing agent. The formation of the silver nanoparticles was noticed using UV- visible absorption spectroscopy. The UV-visible spectroscopy revealed the formation of silver nanoparticles by exhibiting the surface plasmon resonance. The bactericidal activity due to silver release from the surface was determined by the modification of conventional diffusion method. Salmonella typhimurium, Serratia sps and Shigella sps were used as test bacteria which are gram-negative type bacteria. Effect of the different sizes of silver nano particles on antibacterial efficiency was discussed. Zones of inhibition were measured after 24 hours of incubation at 37 °C which gave 20 mm radius for high concentration of silver nanoparticles.

  20. Design and synthesis of an indol derivative as antibacterial agent against Staphylococcus aureus.

    Science.gov (United States)

    Lenin, Hau-Heredia; Lauro, Figueroa-Valverde; Marcela, Rosas-Nexticapa; Socorro, Herrera-Meza; Maria, López-Ramos; Francisco, Díaz-Cedillo; Elodia, García-Cervera; Eduardo, Pool-Gómez; Josefa, Paat-Estrella; Regina, Cauich-Carrillo; Saidy, Euan-Hau

    2017-10-01

    Several indole derivatives with antibacterial activity have been prepared using different protocols; however, some require special reagents and conditions. The aim of this study involved the synthesis of some indole derivatives using estrone and OTBS-estrone as chemical tools. The synthesis of the indole derivatives involves reactions such as follows: (1) synthesis of two indol derivatives ( 4 or 5 ) by reaction of estrone or OTBS-estrone with phenylhydrazine in medium acid; (2) reaction of 4 or 5 with 6-cloro-1-hexyne in medium basic to form two hexynyl-indol ( 7 or 8 ); (3) preparation of indol-propargylic alcohol derivatives ( 10 or 11 ) by reaction of benzaldehyde with 7 or 8 in medium basic; (4) synthesis of indol-aldehydes ( 12 or 13 ) via oxidation of 10 or 11 with DMSO; (5) synthesis of indeno-indol-carbaldehyde ( 15 or 16 ) via alkynylation/cyclization of 12 or 13 with hexyne in presence of copper(II); (6) preparation indeno-indol-carbaldehyde complex ( 19 or 20 ) via alkynylation/cyclization of 12 or 13 with 1-(hex-5-yn-1-yl)-2-phenyl-1 H -imidazole. The antibacterial effect exerted by the indol-steroid derivatives against Streptococcus pneumoniae and Staphylococcus aureus bacteria was evaluated using dilution method and the minimum inhibitory concentration (MIC). The results showed that only the compound 19 inhibit the growth bacterial of S. aureus . In conclusion, these data indicate that antibacterial activity of 19 can be due mainly to functional groups involved in the chemical structure in comparison with the compounds studied.

  1. Synthesis and antibacterial activity of sulfonamide derivatives at C-8 ...

    Indian Academy of Sciences (India)

    pounds. Anacardic acid (pentadecyl salicylic acid) is a phenolic constituent present in CNSL; (Anacardium occidentale L.) and exhibits antimicrobial properties2,3 which have led to the preparation of various ana- logues.23,24 Synthesis of lasiodiplodin from the non- isoprenoid phenolic lipids of CNSL as well as the sali-.

  2. Shape-controlled synthesis of Cu2O nano/microcrystals and their antibacterial activity

    Science.gov (United States)

    Li, Binjie; Li, Yuanyuan; Zhao, Yanbao; Sun, Lei

    2013-12-01

    Uniform cuprous oxides with different morphologies have been successfully synthesized using polyvinylpyrrolidone (PVP) as a capping agent. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis spectrophotometer, Fourier transform infrared spectrometer (FTIR) and X-ray photoelectron spectroscopy were employed to characterize the structure and morphology of cuprous oxides. It was found that the reaction conditions such as PVP, reducing agent and complexing agent played important roles in the formation of regular cuprous oxide crystals. In addition, their antibacterial activity against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) was also investigated by the Oxford cup method. Results suggested that cuprous oxides are selective in their antibacterial action. They display effective antibacterial activity against S. aureus, B. subtilis and P. aeruginosa. There is no bactericidal ability against E. coli in the tested concentration range, which indicates that E. coli may be a Cu(I)-tolerant bacterium.

  3. Design, synthesis and antibacterial activity of isatin derivatives as FtsZ inhibitors

    Science.gov (United States)

    Lian, Zhi-Min; Sun, Juan; Zhu, Hai-Liang

    2016-08-01

    Seven isatin derivatives have been designed, and their chemical structures were characterized by single crystal X-ray diffraction studies, 1H NMR, MS, and elemental analysis. Structural stabilization followed by intramolecular as well as intermolecular H-bonds makes these molecules as perfect examples in molecular recognition with self-complementary donor and acceptor units within a single molecule. These compounds were evaluated for antimicrobial activities. Docking simulations have been performed to position compounds into the FtsZ active site to determine their probable binding models. All of the compounds exhibited better antibacterial activities. Interestingly, compound 5c and 5d exhibited better antibacterial activities with IC50 values of 0.03 and 0.05 μmol/mL against Staphylococcus aureus, respectively. Compound 5g displays antibacterial activity with IC50 values of 0.672 and 0.830 μmol/mL against Escherichia coli and Pseudomonas aeruginosa, respectively.

  4. Trichoderma koningii assisted biogenic synthesis of silver nanoparticles and evaluation of their antibacterial activity

    Science.gov (United States)

    Tripathi, R. M.; Gupta, Rohit Kumar; Shrivastav, Archana; Singh, M. P.; Shrivastav, B. R.; Singh, Priti

    2013-09-01

    The present study demonstrates the biosynthesis of silver nanoparticles using Trichoderma koningii and evaluation of their antibacterial activity. Trichoderma koningii secretes proteins and enzymes that act as reducing and capping agent. The biosynthesized silver nanoparticles (AgNPs) were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and x-ray diffraction (XRD). UV-Vis spectra showed absorbance peak at 413 nm corresponding to the surface plasmon resonance of silver nanoparticles. DLS was used to find out the size distribution profile. The size and morphology of the AgNPs was determined by TEM, which shows the formation of spherical nanoparticles in the size range of 8-24 nm. X-ray diffraction showed intense peaks corresponding to the crystalline silver. The antibacterial activity of biosynthesized AgNPs was evaluated by growth curve and inhibition zone and it was found that the AgNPs show potential effective antibacterial activity.

  5. Synthesis, Characterization and Antimicrobial Activities of Some ...

    African Journals Online (AJOL)

    Some transition metal complexes of nicotinamide have been prepared and characterized using melting point, conductivity measurement, infrared, electronic, HNMR and atomic absorption spectroscopic methods. . The antibacterial and antifungal studies of the metal complexes and the ligand have been evaluated against ...

  6. Synthesis, antioxidant and antibacterial activities of 3-nitrophenyl ferrocene

    Science.gov (United States)

    Benabdesselam, S.; Izza, H.; Lanez, T.; Guechi, E. K.

    2018-03-01

    The current work aims in its first part to synthesize 3-nitrophenylferrocene after diazotizing nitroaniline in the meta position by the sodium nitrite and the formation of the corresponding diazonium salt: 3-nitrobenzendiazonium sulfate, then the salt in solution was added to the ferrocene for the purpose of introducing the nitrophenyl moiety thereon (arylation) and the formation of 3-nitrophenylferrocene. The second part is devoted to the study of the antioxidant activity of 3-NPF by applying the trapping test of superoxide radical using cyclic voltammetry, the free radical DPPH trapping test by spectrophotometry. The results showed that 3-nitrophenylferrocene has a scavenging effect of DPPH radical with IC50 = 1.44mg/ml, superoxide radical with IC50=5.38mg/ml. The third part is devoted to the study of antibacterial activity of the synthesized compound tested on four strains of bacteria: Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Klebsiella pneumoniae. The obtained results clearly showed that 3-nitrophenylferrocene has low activities on the four bacterial strains with diameters of inhibition zones do not exceeding 17 mm at concentrations of 25mg/ml.

  7. Isolation and Characterization of Antibacterial Compounds from Sea Urchin

    Directory of Open Access Journals (Sweden)

    Febrina Olivia Akerina

    2015-06-01

    Full Text Available Sea urchin is highly valued seafood. Its bioactive compound also has a potential as natural antibacterial agent. The aim of this research was to screen the antibacterial activity using cup-plate method, toxicity potential using brine shrimp lethality test (BSLT method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50 from the three gonads extract was 471.861 ppm (n-hexane, 563.226 ppm (ethyl acetate and 577.531 ppm (methanolic, respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli. Keyword : Antibacteria, bioactive compound, D. setosum, sea urchin

  8. Synthesis and biological evaluation of coumarin derivatives containing imidazole skeleton as potential antibacterial agents.

    Science.gov (United States)

    Hu, Yang; Shen, Yufeng; Wu, Xiaohu; Tu, Xiao; Wang, Gao-Xue

    2018-01-01

    Emergence of multidrug-resistant bacteria causes an urgent need for new generation of antibiotics, which may have a different mechanism of inhibition or killing action from the existing. Here, we report on the design, synthesis, and biological evaluation of thirty-nine coumarin derivatives in order to solve the antibacterial resistance by targeting at the inhibition of biosynthesis pathway of fatty acids. Their antibacterial activities against Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, and Flavobacterium cloumnare are tested and action mechanism against the key enzyme in bacterial fatty acid synthesis pathway are studied. The results show that compounds 13 and 18 have potent and broad spectrum antimicrobial activity. In addition, 9, 14 and 19 show eminent antimicrobial efficacy toward S. aureus, S. agalactiae, and F. cloumnare. Mechanistically, coumarin derivatives display the antibacterial activity via the control of FabI and FabK function. The structure-activity relationship analysis indicate that the length of linker and imidazole substitute group could significantly influence the antimicrobial activity, as well as the inhibitory activity against FabI and FabK. The structural optimization analysis of coumarin suggest that derivatives 9, 13, 14, 18 and 19 could be a viable way of preventing and controlling bacteria and considered as promising lead compounds for the development of commercial drugs. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  9. Study of mechanism of enhanced antibacterial activity by green synthesis of silver nanoparticles

    Science.gov (United States)

    Parashar, Upendra Kumar; Kumar, Vinod; Bera, Tanmay; Saxena, Preeti S.; Nath, Gopal; Srivastava, Sunil K.; Giri, Rajiv; Srivastava, Anchal

    2011-10-01

    The extensive use of silver nanoparticles needs a synthesis process that is greener without compromising their properties. The present study describes a novel green synthesis of silver nanoparticles using Guava (Psidium guajava) leaf extract. In order to compare with the conventionally synthesized ones, we also prepared Ag-NPs by chemical reduction. Their optical and morphological characteristics were thoroughly investigated and tested for their antibacterial properties on Escherichia coli. The green synthesized silver nanoparticles showed better antibacterial properties than their chemical counterparts even though there was not much difference between their morphologies. Fourier transform infrared (FTIR) spectroscopic analysis of the used extract and as-synthesized silver nanoparticles suggests the possible reduction of Ag + by the water-soluble ingredients of the guava leaf like tannins, eugenol and flavonoids. The possible reaction mechanism for the reduction of Ag + has been proposed and discussed. The time-dependent electron micrographs and the simulation studies indicated that a physical interaction between the silver nanoparticles and the bacterial cell membrane may be responsible for this effect. Based on the findings, it seems very reasonable to believe that this greener way of synthesizing silver nanoparticles is not just an environmentally viable technique but it also opens up scope to improve their antibacterial properties.

  10. Study of mechanism of enhanced antibacterial activity by green synthesis of silver nanoparticles

    International Nuclear Information System (INIS)

    Parashar, Upendra Kumar; Srivastava, Sunil K; Srivastava, Anchal; Kumar, Vinod; Saxena, Preeti S; Bera, Tanmay; Nath, Gopal; Giri, Rajiv

    2011-01-01

    The extensive use of silver nanoparticles needs a synthesis process that is greener without compromising their properties. The present study describes a novel green synthesis of silver nanoparticles using Guava (Psidium guajava) leaf extract. In order to compare with the conventionally synthesized ones, we also prepared Ag-NPs by chemical reduction. Their optical and morphological characteristics were thoroughly investigated and tested for their antibacterial properties on Escherichia coli. The green synthesized silver nanoparticles showed better antibacterial properties than their chemical counterparts even though there was not much difference between their morphologies. Fourier transform infrared (FTIR) spectroscopic analysis of the used extract and as-synthesized silver nanoparticles suggests the possible reduction of Ag + by the water-soluble ingredients of the guava leaf like tannins, eugenol and flavonoids. The possible reaction mechanism for the reduction of Ag + has been proposed and discussed. The time-dependent electron micrographs and the simulation studies indicated that a physical interaction between the silver nanoparticles and the bacterial cell membrane may be responsible for this effect. Based on the findings, it seems very reasonable to believe that this greener way of synthesizing silver nanoparticles is not just an environmentally viable technique but it also opens up scope to improve their antibacterial properties.

  11. Study of mechanism of enhanced antibacterial activity by green synthesis of silver nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Parashar, Upendra Kumar; Srivastava, Sunil K; Srivastava, Anchal [Department of Physics, Banaras Hindu University, Varanasi 221005 (India); Kumar, Vinod; Saxena, Preeti S [Department of Zoology, Banaras Hindu University, Varanasi 22005 (India); Bera, Tanmay [Department of Mechanical, Materials and Aerospace Engineering, University of Central Florida, Orlando, FL 32816 (United States); Nath, Gopal [Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 22005 (India); Giri, Rajiv, E-mail: anchalbhu@gmail.com [Department of Materials Science and Engineering, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway)

    2011-10-14

    The extensive use of silver nanoparticles needs a synthesis process that is greener without compromising their properties. The present study describes a novel green synthesis of silver nanoparticles using Guava (Psidium guajava) leaf extract. In order to compare with the conventionally synthesized ones, we also prepared Ag-NPs by chemical reduction. Their optical and morphological characteristics were thoroughly investigated and tested for their antibacterial properties on Escherichia coli. The green synthesized silver nanoparticles showed better antibacterial properties than their chemical counterparts even though there was not much difference between their morphologies. Fourier transform infrared (FTIR) spectroscopic analysis of the used extract and as-synthesized silver nanoparticles suggests the possible reduction of Ag{sup +} by the water-soluble ingredients of the guava leaf like tannins, eugenol and flavonoids. The possible reaction mechanism for the reduction of Ag{sup +} has been proposed and discussed. The time-dependent electron micrographs and the simulation studies indicated that a physical interaction between the silver nanoparticles and the bacterial cell membrane may be responsible for this effect. Based on the findings, it seems very reasonable to believe that this greener way of synthesizing silver nanoparticles is not just an environmentally viable technique but it also opens up scope to improve their antibacterial properties.

  12. Synthesis of selected 5-thio-substituted tetrazole derivatives and evaluation of their antibacterial and antifungal activities

    Directory of Open Access Journals (Sweden)

    NALILU SUCHETHA KUMARI

    2011-02-01

    Full Text Available Several 5-thio-substituted tetrazole derivatives were efficiently synthesized by a three-step process. The substituted tetrazol-5-thiol, namely, 1-benzyl-1H-tetrazole-5-thiol (2 was prepared by refluxing commercially available benzyl isothiocyanate (1 with sodium azide in water. The second step was the synthesis of 1-benzyl-5-[(3-bromopropylthio]-1H-tetrazole (3 by thioalkylation of tetrazole-5-thiol 2 with 1,3-dibromopropane in tetrahydrofuran. Finally, the 5-thio-substituted tetrazole derivatives 4a–i were prepared by condensation of 3 with the corresponding amine or thiol. The structures of the newly synthesized compounds were characterized by NMR, LC/MS/MS, IR spectral data and elemental analysis. All the synthesized compounds were screened for their antibacterial and antifungal activities.

  13. Synthesis of glycerol mono-laurate from lauric acid and glycerol for food antibacterial additive

    Science.gov (United States)

    Setianto, W. B.; Wibowo, T. Y.; Yohanes, H.; Illaningtyas, F.; Anggoro, D. D.

    2017-05-01

    Synthesis of glycerol mono-laurate (GML) has been performed using esterification reaction of glycerol and lauric acid. The reaction was performed at the condition of temperature of 120-140 °C within 7 hour, variation of molar ratio of glycerol - lauric acid, and was using heterogeneous catalyst of zeolist Y. Without catalyst dealumination the maximum acid conversion was 78%, with GML contained in the sample was 38.6%, and it was obtained at the reaction condition of 140 oC, 15wt% catalyst, and 8:1 molar ratio of glycerol - lauric acid. At the same condition, using dealuminated catalyst, the maximum acid conversion was increased up to 98%, with GML contained in the sample was 50.4%. The GML antibacterial activity was examined. It was observed that the GML has antibacterial activity against gram positive bacterial such as B. cereus and S. aureus.

  14. Efficient Synthesis and Antibacterial Evaluation of (±-Yanglingmycin and Its Analogues

    Directory of Open Access Journals (Sweden)

    Wenjia Dan

    2016-01-01

    Full Text Available An efficient synthetic route was developed for the large-scale preparation of (±-Yanglingmycin and its analogues. Three series of derivatives of (±-Yanglingmycin were synthesized and the structures of all compounds were elucidated by analyses of NMR and ESI-MS spectra data. Moreover, their antibacterial activities against seven species of bacteria were systematically evaluated by the micro-broth dilution method, most of which displayed considerable activity. It was worth noting that compounds 5b, 5c, 5d, 6g, and 7 were found to be the most promising leading candidates, with peak MIC values of 0.98 μg·mL−1 for Bacillus subtilis, which is superior to positive controls (MIC = 3.91 μg·mL−1. The above results might lay the firm foundation for the design and synthesis of novel antibacterial drugs based on (±-Yanglingmycin.

  15. Preparation, characterization and antibacterial effects of eco-friendly ...

    African Journals Online (AJOL)

    688. 14. Daglia M, Papetti A, Grisoli P, Aceti C, Dacarro C,. Gazzani G. Antibacterial activity of red and white wine against oral streptococci. J. Agric. Food Chem. 2007;. 55: 5038–5042. 15. Jarvinen H, Tenovuo J, Huovinen P. In vitro susceptibility.

  16. Isolation and Characterization of Antibacterial Compounds from Sea Urchin

    Directory of Open Access Journals (Sweden)

    Febrina Olivia Akerina,

    2015-06-01

    Full Text Available method, toxicity potential using brine shrimp lethality test (BSLT method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50 from the three gonads extract was 471.861 ppm (n-hexane, 563.226 ppm (ethyl acetate and 577.531 ppm (methanolic, respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli.

  17. Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

    Directory of Open Access Journals (Sweden)

    Arokiyaraj S

    2014-01-01

    Full Text Available Selvaraj Arokiyaraj,1 Mariadhas Valan Arasu,2 Savariar Vincent,3 Nyayirukannaian Udaya Prakash,4 Seong Ho Choi,5 Young-Kyoon Oh,1 Ki Choon Choi,2 Kyoung Hoon Kim1,61Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea; 2Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Seonghwan-Eup, Cheonan-Si, Chungnam, Republic of Korea; 3Center for Environmental Research and Development, Loyola College, Chennai, India; 4Research and Development, Vel Tech Dr RR and Dr SR Technical University, Chennai, India; 5Department of Animal Science, Chungbuk National University, Chungbuk, Republic of Korea; 6Department of Animal Science, Seoul National University, Pyeongchang, Republic of KoreaAbstract: The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet–visible spectroscopy (435 nm. The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71–71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa. Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo

  18. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract.

    Science.gov (United States)

    Al-Abd, Nazeh M; Mohamed Nor, Zurainee; Mansor, Marzida; Azhar, Fadzly; Hasan, M S; Kassim, Mustafa

    2015-10-24

    The threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. The antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. Therefore, this study evaluated the phytochemical constituents of Melaleuca cajuputi flower and leaf (GF and GL, respectively) extracts and their antioxidant and antibacterial activities. Radical scavenging capacity of the extracts was estimated using 2,2-diphenyl-2-picrylhydrazyl and Fe(2+)-chelating activity. Total antioxidant activity was determined using ferric reducing antioxidant power assay. Well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration assays were used to determine antibacterial activity against eight pathogens, namely Staphylococcus aureus, Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Salmonella typhimurium, Klebsiella pneumonia, Streptococcus pneumoniae, and Pasteurella multocida. We identified and quantified the phytochemical constituents in methanol extracts using liquid chromatography/mass spectrometry (LC/MS) and gas chromatography (GC)/MS. This study reports the antioxidant and radical scavenging activity of M. cajuputi methanolic extracts. The GF extract showed better efficacy than that of the GL extract. The total phenolic contents were higher in the flower extract than they were in the leaf extract (0.55 ± 0.05 and 0.37 ± 0.05 gallic acid equivalent per mg extract dry weight, respectively). As expected, the percentage radical inhibition by GF was higher than that by the GL extract (81 and 75 %, respectively). A similar trend was observed in Fe(2+)-chelating activity and β-carotene bleaching tests. The antibacterial assay of the extracts revealed no inhibition zones with the Gram-negative bacteria tested. However, the extracts demonstrated activity against B. cereus, S. aureus, and S. epidermidis. In

  19. Green synthesis of silver nanoparticle using rambutan (Nephelium lappaceum L. peel extract and its antibacterial activity against Salmonella parathypi A.

    Directory of Open Access Journals (Sweden)

    Lestari Puji

    2018-01-01

    Full Text Available Ag nanoparticles (AgNPs have been synthesized via green method using rambutan (Nephelium lappaceum L. peel (RP extract. RP extract was prepared by washing the RP using tap water thoroughly and boiling it in distilled water at 70°C for 60 min. RP extract and AgNO3 were used as the starting materials for the synthesis of AgNPs. RP extract was added to 10-3 M AgNO3 solution with a ratio by volume of 1:10 (RP : AgNO3, stirred at room temperature. The solution’s color changes from reddish to dark brown, indicating the reduction of Ag+ in the solution. The synthesized AgNPs were characterized using UV-Visible Spectrophotometer, FTIR Spectrophotometer, and Scanning Electron Microscopy-Energy-dispersive X-ray Spectroscopy (SEM-EDS instruments. UV-Visible spectra show that the AgNPs have the maximum absorption band at 450 nm which is typical for AgNPs. The FTIR spectra revealed that the protein in RP extract acts as the capping agent for the synthesized AgNPs. The synthesized AgNPs were tested for their antibacterial activity against Salmonella parathypi A. The antibacterial test shows that 50 μL of AgNPs resulted in the inhibition zone of 4 mm against the aforementioned microorganism.

  20. Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides and Its Antibacterial Activity

    Directory of Open Access Journals (Sweden)

    Semra Cicek

    2015-01-01

    Full Text Available Silver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified from Euphorbia (Euphorbia amygdaloides plant. Optimum pH, temperature and time period were determined to obtain silver nanoparticles using the peroxidase enzyme. The result shows that higher silver nanoparticle was synthesized for 4 hours and at 20°C and pH 8. Also, optimal concentration of metal ions was found as 0.5 mM. The synthesized silver nanoparticles were characterized by UV spectrum, scanning electron microscope (SEM and X-ray diffraction. Antibacterial activity of silver nanoparticles was measured against some microorganisms such as Serratia marcescens, Yersinia pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. Synthesized silver nanoparticles have wide spectrum antibacterial activity in low concentration and may be a good alternative therapeutic approach in medicine and pharmaceutical fields in future.

  1. Synthesis of Silver Nanoparticles from the Aqueous Extract of Leaves of Ocimum sanctum for Enhanced Antibacterial Activity

    Directory of Open Access Journals (Sweden)

    Charusheela Ramteke

    2013-01-01

    Full Text Available The field of nanotechnology is the most active area of research in modern materials science. Though there are many chemical as well as physical methods, green synthesis of nanomaterials is the most emerging method of synthesis. We report the synthesis of antibacterial silver nanoparticles (AgNPs using leaf broth of medicinal herb, Ocimum sanctum (Tulsi. The synthesized AgNPs have been characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM, and X-ray diffractometry. The mean particle of synthesized NPs was found to be 18 nm, as confirmed by TEM. The qualitative assessment of reducing potential of leaf extract has also been carried out which indicated presence of significant amount of reducing entities. FTIR analysis revealed that the AgNPs were stabilized by eugenols, terpenes, and other aromatic compounds present in the extract. Such AgNPs stabilized by Tulsi leaf extract were found to have enhanced antimicrobial activity against well-known pathogenic strains, namely Staphylococcus aureus and E. coli.

  2. Facile synthesis, stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates.

    Science.gov (United States)

    Lukman, Audra I; Gong, Bin; Marjo, Christopher E; Roessner, Ute; Harris, Andrew T

    2011-01-15

    The biogenic synthesis of metal nanomaterials offers an environmentally benign alternative to the traditional chemical synthesis routes. Colloidal silver (Ag) nanoparticles were synthesized by reacting aqueous AgNO(3) with Medicago sativa seed exudates under non-photomediated conditions. Upon contact, rapid reduction of Ag(+) ions was observed in nanotriangles with edge lengths of 86-108 nm, while pH adjustment to 11 resulted in monodisperse Ag nanoparticles with an average size of 12 nm. Repeated centrifugation and redispersion enhanced the percentage of nanoplates from 10% to 75% in solution. The kinetics of nanoparticle formation were monitored using ultraviolet-visible spectroscopy and the Ag products were characterized using transmission electron microscopy, selected-area electron diffraction, scanning electron microscopy, X-ray powder diffraction, and atomic force microscopy. X-ray photoelectron spectroscopy was used to investigate the elements and chemical environment in the top layers of the as-synthesized Ag nanoparticles, while the metabolites in the exudate were analyzed using gas chromatography-mass spectroscopy. To our knowledge, this is the first account of M. sativa seed exudate assisted synthesis and stabilization of biogenic Ag nanoparticles; the nanoplates are notably smaller and better faceted compared with those synthesized by vascular plant extracts previously reported. Stabilized films of exudate synthesized Ag nanoparticles were effective anti-bacterial agents. Copyright © 2010 Elsevier Inc. All rights reserved.

  3. Synthesis and studies of antibacterial activity of pongaglabol

    Indian Academy of Sciences (India)

    Unknown

    Introduction. Pongamia glabra Vent. (Leguminosae) is one of the most commonest and useful medicinal plants of In- dia.1. The isolation and characterization of pon- ..... gave the corresponding chalcone 5 in good yield as yellow needles; m.p. 151–152°C. The spectral data. (UV, IR and 1H NMR) confirmed compound 5 as.

  4. Synthesis and characterization of Taurine

    Directory of Open Access Journals (Sweden)

    B Bayarmaa

    2014-10-01

    Full Text Available Have been obtained 2-aminoethanesulfonic acid (taurine from ethanolamine, sulfuric acid and sodium sulfite during the synthesis in laboratory condition. The process involves two steps of reactions, the first was esterification of ethanolamine with sulfuric acid to produce the intermediate product of 2-aminoethyl ester which than was extended to the second step by sulfonation with sodium sulfite to produce 2-aminoethanesulfonic acid. Resulting product was analyzed using 1H-NMR, IR, FAB-MS analysis and examined purity characterizations of the synthesized products. DOI: http://dx.doi.org/10.5564/mjc.v14i0.200 Mongolian Journal of Chemistry 14 (40, 2013, p57-60

  5. Synthesis and Anti-Bacterial Activities of Some Novel Schiff Bases Derived from Aminophenazone

    Directory of Open Access Journals (Sweden)

    Salman A Khan

    2010-10-01

    Full Text Available A series of 1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one-containing Schiff bases were synthesized, characterized and screened for their antibacterial activities. The structures of the synthesized compounds were established by spectroscopic (FT-IR, 1H-NMR, 13C-NMR, MS and elemental analyses. The anti-bacterial activities (with MIC values of compounds were evaluated. The anti-bacterial screening results reveal that among the six compounds screened, four compounds showed moderate to good anti-bacterial activity. Among the tested compounds, the most effective compounds against four bacterial strains, viz. Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Streptococcus pyogenes, are [(2-Chlorobenzylideneamino]-1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one (4 and [(1,5-Dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yliminomethyl]benzonitrile (5 with MIC values of 6.25 μg/mL.

  6. Chiral lactic hydrazone derivatives as potential bioactive antibacterial agents: Synthesis, spectroscopic, structural and molecular docking studies

    Science.gov (United States)

    Noshiranzadeh, Nader; Heidari, Azam; Haghi, Fakhri; Bikas, Rahman; Lis, Tadeusz

    2017-01-01

    A series of novel chiral lactic-hydrazone derivatives were synthesized by condensation of (S)-lactic acid hydrazide with salicylaldehyde derivatives and characterized by elemental analysis and spectroscopic studies (FT-IR, 1H NMR and 13C NMR spectroscopy). The structure of one compound was determined by single crystal X-ray analysis. Antibacterial activity of the synthesized compounds was studied against Staphylococcus aureus, Streptococcus pneumonia, Escherichia coli and Pseudomonas aeruginosa as bacterial cultures by broth microdilution method. All of the synthesized compounds showed good antibacterial activity with MIC range of 64-512 μg/mL. Compounds (S,E)-2-hydroxy-N-(2-hydroxy-5-nitrobenzylidene)propanehydrazide (5) and (S,E)-2-hydroxy-N-((3-hydroxy-5-(hydroxymethyl)-2-methylpyridin-4-yl)propanehydrazide (7) were the most effective antibacterial derivatives against S. aureus and E. coli respectively with a MIC value of 64 μg/mL. Bacterial biofilm formation assay showed that these compounds significantly inhibited biofilm formation of P. aeruginosa. Also, in silico molecular docking studies were performed to show lipoteichoic acid synthase (LtaS) inhibitory effect of lactic hydrazone derivatives. The association between electronic and structural effects of some substituents on the benzylidene moiety and the biological activity of these chiral compounds were studied. Structural studies show that compound with higher hydrogen bonding interactions show higher antibacterial activity. The results show chiral hydrazone derivatives based on lactic acid hydrazide could be used as potential lead compounds for developing novel antibacterial agents.

  7. Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity.

    Science.gov (United States)

    Erci, Fatih; Cakir-Koc, Rabia; Isildak, Ibrahim

    2017-12-17

    Silver (Ag) nanoparticles (NPs) were green synthesized at room temperature using different concentrations of the Thymbra spicata L. var. spicata (Zahter) aqueous leaf extracts for the first time. With the synthesis of AgNPs using the leaf extract of Cynara scolymus (Artichoke) and Mentha piperita (Peppermint), the biological activities of the nanoparticles synthesized using leaf extract of three economically significant plants have been studied comparatively. Nanoparticles were characterized by different spectroscopic and microscopic analysis. TEM analysis of the biosynthesized AgNPs revealed that the size and shape of the AgNPs were changed with the plant extract concentration. Biologically synthesized AgNPs from leaf extracts of the three different plants displayed significant differences in antibacterial activity against two different gram-negative and gram-positive bacteria. Also, the results from this study show the shape dependence of the antibacterial and cytotoxic activity of silver nanoparticles synthesized using T. spicata leaf extract. The nanoparticles with different shapes exhibited the strongest antibacterial and cytotoxic activity compared to mostly spherical nanoparticles. Present results clearly indicate that biological activities of silver nanoparticles were affected by nanoparticle shape and the source of the plant extract used in the synthesis.

  8. Synthesis and antibacterial studies of 2-aryl-3-alkanamido-4H-thiazolidin-4-one derivatives

    Directory of Open Access Journals (Sweden)

    Sandeep Jain

    2016-09-01

    Full Text Available A series of 2-aryl-3-alkanamido-4H-thiazolidin-4-ones were synthesized from long chain fatty acid hydrazides and studied for their in vitro antibacterial activity. Long chain fatty acid hydrazides 1 on reaction with different aromatic aldehydes in the presence of catalytic amount of glacial acetic acid yielded the corresponding aryl hydrazones 2 which on further reaction with thioglycolic acid in the presence of anhydrous zinc chloride furnished the title compounds 3. These compounds were characterized by CHN analyses, IR, mass and 1H NMR spectral data. All the compounds were evaluated for their in vitro antibacterial activity against two Gram positive strains (Bacillus subtilis and Staphylococcus aureus and two Gram negative strains (Escherichia coli and Pseudomonas aeruginosa and their minimum inhibitory concentration (MIC were determined.

  9. Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity

    Directory of Open Access Journals (Sweden)

    Pandiyan eRajeswari

    2015-01-01

    Full Text Available A moderately halotolerent Streptomyces strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant Streptomyces sp. JAJ13. Novelty of the stain JAJ13 in production of antibacterial compound was assessed by sequence analysis of KSα gene and LC-MS analysis of the active compound. Optimization of the culture medium for antibacterial compound production by the strain JAJ13 was performed with statistical methodology based on experimental designs. Initially, a starch based basal production medium was selected out of eight different production media screened for antimicrobial compound production by Streptomyces sp. JAJ13. Plackett-Burman design was employed to screen the influential media components affecting the antibacterial compound production. Subsequently, statistical optimization of selected medium components was performed by employing the Response Surface Methodology (RSM with Box-Behnken design. The optimum initial level of CuSO4.5H2O, (NH42SO4 and K2HPO4 for the highest antibacterial activity was determined to be at 4.45 mg, 1.96 g and 1.15 g in 1 L of distilled H2O, respectively. PBD and RSM guided design of experiments resulted in a maximum antibacterial activity of 23.37 ± 2.08 mm, which is a 78.8 % increase in comparison with that obtained in the unoptimized medium. This study points the success of statistical model in developing an optimized production media for enhanced antibacterial compound production by Streptomyces sp. JAJ13.

  10. Synthesis and characterization of saturated polyester and ...

    Indian Academy of Sciences (India)

    Synthesis and characterization of saturated polyester and nanocomposites derived from glycolyzed PET waste ... construction industries. PET is widely used in the packaging of beverages and drugs. ... ing the synthesis of saturated polyester (from GPET waste). This has been done for the estimation of the maximum. 277 ...

  11. Synthesis, characterization and application of semiconducting oxide ...

    Indian Academy of Sciences (India)

    Nanostructured; Cu2O nanostructures; electrolysis based oxidation; aligned ZnO nanorods. Abstract. In the present study, we report the synthesis, characterization and application of nanostructured oxide materials. The oxide ... The copper electrode served as a sacrificial anode for the synthesis of different nanostructures.

  12. Synthesis, characterization, and antimicrobial properties of copper nanoparticles

    Science.gov (United States)

    Usman, Muhammad Sani; Zowalaty, Mohamed Ezzat El; Shameli, Kamyar; Zainuddin, Norhazlin; Salama, Mohamed; Ibrahim, Nor Azowa

    2013-01-01

    Copper nanoparticle synthesis has been gaining attention due to its availability. However, factors such as agglomeration and rapid oxidation have made it a difficult research area. In the present work, pure copper nanoparticles were prepared in the presence of a chitosan stabilizer through chemical means. The purity of the nanoparticles was authenticated using different characterization techniques, including ultraviolet visible spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The antibacterial as well as antifungal activity of the nanoparticles were investigated using several microorganisms of interest, including methicillin-resistant Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella choleraesuis, and Candida albicans. The effect of a chitosan medium on growth of the microorganism was studied, and this was found to influence growth rate. The size of the copper nanoparticles obtained was in the range of 2–350 nm, depending on the concentration of the chitosan stabilizer. PMID:24293998

  13. Synthesis and characterization of tetraethylammonium tetrachloro ...

    Indian Academy of Sciences (India)

    Unknown

    Synthesis and characterization of tetraethylammonium tetrachloro- cobaltate crystals. M A KANDHASWAMY and V SRINIVASAN*. Department of Physics, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore 641 020, India. MS received 7 February 2000; revised 27 December 2001. Abstract.

  14. Biological synthesis and characterization of silver nanoparticles ...

    Indian Academy of Sciences (India)

    Biological synthesis and characterization of silver nanoparticles using. Eclipta alba leaf extract and evaluation of its cytotoxic and antimicrobial potential. PARAMASIVAM PREMASUDHA1, MUDILI VENKATARAMANA2,∗, MARRIAPPAN ABIRAMI3,. PERIYASAMY VANATHI4, KADIRVELU KRISHNA2 and RAMASAMY ...

  15. Antibacterial Barbituric Acid Analogues Inspired from Natural 3-Acyltetramic Acids; Synthesis, Tautomerism and Structure and Physicochemical Property-Antibacterial Activity Relationships

    Directory of Open Access Journals (Sweden)

    Yong-Chul Jeong

    2015-02-01

    Full Text Available The synthesis, tautomerism and antibacterial activity of novel barbiturates is reported. In particular, 3-acyl and 3-carboxamidobarbiturates exhibited antibacterial activity, against susceptible and some resistant Gram-positive strains of particular interest is that these systems possess amenable molecular weight, rotatable bonds and number of proton-donors/acceptors for drug design as well as less lipophilic character, with physicochemical properties and ionic states that are similar to current antibiotic agents for oral and injectable use. Unfortunately, the reduction of plasma protein affinity by the barbituric core is not sufficient to achieve activity in vivo. Further optimization to reduce plasma protein affinity and/or elevate antibiotic potency is therefore required, but we believe that these systems offer unusual opportunities for antibiotic drug discovery.

  16. The characterization of the antibacterial efficacy of an electrically activated silver ion-based surface system

    Science.gov (United States)

    Shirwaiker, Rohan A.

    There have been growing concerns in the global healthcare system about the eradication of pathogens in hospitals and other health-critical environments. The problem has been aggravated by the overuse of antibiotics and antimicrobial agents leading to the emergence of antibiotic-resistant superbugs such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) which are difficult to kill. Lower immunity of sick patients coupled with the escalating concurrent problem of antibiotic-resistant pathogens has resulted in increasing incidences of hospital acquired (nosocomial) infections. There is an immediate need to control the transmission of such infections, primarily in healthcare environments, by creating touch-contact and work surfaces (e.g., door knobs, push plates, countertops) that utilize alternative antibacterial materials like the heavy metal, silver. Recent research has shown that it is silver in its ionic (Ag+ ) and not elemental form that is antibacterial. Thus, silver-based antibacterial surfaces have to release silver ions directly into the pathogenic environment (generally, an aqueous media) in order to be effective. This dissertation presents the study and analysis of a new silver-based surface system that utilizes low intensity direct electric current (LIDC) for generation of silver ions to primarily inhibit indirect contact transmission of infections. The broader objective of this research is to understand the design, and characterization of the electrically activated silver ion-based antibacterial surface system. The specific objectives of this dissertation include: (1) Developing a comprehensive system design, and identifying and studying its critical design parameters and functional mechanisms. (2) Evaluating effects of the critical design parameters on the antibacterial efficacy of the proposed surface system. (3) Developing a response surface model for the surface system performance. These objectives are

  17. Green synthesis of silver nanoparticle using echinops extract and its antibacterial activity

    Directory of Open Access Journals (Sweden)

    Fatemeh Nakhjiri

    2017-04-01

    Full Text Available Objective(s: Silver nanoparticles (Ag NPs are not only specific physical and chemical properties but also are considered for their antibacterial activity and ecofriendly.Materials and Methods:In this study a simple, cost effective biologically method for Ag+reducing to Ag NPs using Echinops extractas a stabilizer, and reducing agent.Ag NPs were analyzed using UV-Vis spectrometry,TEM, XRD and FTIR. The role of Echinops concentration, silver nitrate concentration, pHand reaction time on the synthesis of nanoparticles were studied. Antibacterial activity of the Ag NPs werecarried out by disc diffusion method against Staphylococcus aureus and Escherichia coli. Alsothe amount of MBC and MIC for AgNPs against bacteria wereinvestigated.Results: The AgNPs formation were observed as a color change of the mixture from colorless to dark-brownish. The UV-Vis spectroscopy absorbance peak at 420 nm confirmed the presence of Ag NPs. TEM analysis, showed Ag NPs were spherical, triangle and bar particles in shape with size range within 1.32-36.41 nm. XRD study showed particles were crystalline in nature. FTIR analysis detected that Ag NPsare functionalized with biomolecules that are present in the aqueous Echinops extractact as the reducing agents and stabilizing the nanoparticles. The results showed that the time of reaction, temperature, pH, Echinops extract concentration and AgNO3concentration could accelerate the formation of AgNPs.Conclusion: In this study, synthesized Ag NPs havethe efficient antibacterial activity against pathogenic bacteria. Ag NPs havean importantfunction in the field of nanotechnology and nanomedicine.

  18. Synthesis and antibacterial activities of novel pleuromutilin derivatives with a substituted pyrimidine moiety.

    Science.gov (United States)

    Yi, Yunpeng; Xu, Ximing; Liu, Yu; Xu, Shuijin; Huang, Xin; Liang, Jianping; Shang, Ruofeng

    2017-01-27

    The alarming growth of multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) has become a major global health hazard. Therefore, urgent demand for new antibiotics with a unique mechanism of action is very necessary. The present study reports the design, synthesis, and antibacterial studies of a series of novel pleuromutilin derivatives with substituted 6-amino pyrimidine moieties. Most of the tested compounds exhibited highly potent anti-MRSA or Staphylococcus aureus (S. aureus) activities. 14-O-[(4,6-Diamino -pyrimidine-2-yl) thioacetyl] mutilin (3) and 14-O-[(2-((3R)-3-Hydroxymethylpiperidine-1-yl)-acetamido-6-aminopyrimidine-2-yl) thioacetyl] mutilin (5h) were the most active compounds and showed higher antibacterial activities. Compound 3 displayed rapid bactericidal activity and affected bacterial growth with the same manner as tiamulin fumarate. Docking experiments for compounds 3 and 5h carried out on the peptidyl transferase center (PTC) of 23S rRNA provided the information about the binding model. In vivo mouse systemic infection experimental results confirmed the therapeutic efficacy of compound 3, with ED 50 of 4.22 mg/kg body weight against MRSA. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  19. Synthesis and concentration dependent antibacterial activities of CuO nanoflakes

    Energy Technology Data Exchange (ETDEWEB)

    Pandiyarajan, T.; Udayabhaskar, R. [Department of Physics, National Institute of Technology, Tiruchirappalli 620 015 (India); Vignesh, S.; James, R. Arthur [Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024 (India); Karthikeyan, B., E-mail: balkarin@yahoo.com [Department of Physics, National Institute of Technology, Tiruchirappalli 620 015 (India)

    2013-05-01

    We report, synthesis and antibacterial activities of CuO nanoflakes. CuO nanoparticles are prepared at room temperature through sol–gel method. X-ray diffraction studies show the particles are monoclinic (crystalline) in nature. Scanning electron microscopy (SEM) images clearly show that the prepared particles are flake like in structure. Fourier transform infrared (FTIR) spectra exhibits three different bands that correspond to the A{sub u} and B{sub u} modes. Antibacterial studies were performed on Shigella flexneri, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella typhimurium, Bacillus subtilis, Escherichia coli, Vibrio cholera, Pseudomonas aeruginosa and Aeromonas liquefaciens bacterial strains. Among these bacterial strains, S. flexneri and B. subtilis are most sensitive to copper oxide nanoparticles than the positive control (Penicillin G) and S. typhimurium strain shows the less sensitive. Results show that sensitivity is highly dependent on the concentrations of CuO nanoflakes. - Highlights: ► CuO nanoflakes are prepared through simple sol–gel method at room temperature. ► Bacterial strains are highly affected by CuO nanoflakes than the positive control. ► Zone of inhibition increases with an increase of CuO concentrations. ► Sensitivity is highly dependent on the concentrations of CuO nanoflakes.

  20. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity

    Science.gov (United States)

    Bhakya, S.; Muthukrishnan, S.; Sukumaran, M.; Muthukumar, M.

    2016-06-01

    Nanomedicine utilizes biocompatible nanomaterials for diagnostic and therapeutic purposes. The present study reports the use of Helicteres isora root extract for the synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were initially noticed through visual color change from yellow to reddish brown and further confirmed by surface plasmonic resonance (SPR) band at 450 nm using UV-visible spectroscopy. Morphology and size of AgNPs were determined by transmission electron microscopy (TEM) analysis. X-ray diffraction (XRD) study revealed crystalline nature of AgNPs. The prolonged stability of AgNPs was due to capping of oxidized polyphenols and carboxyl protein which was established by Fourier transform infrared spectroscopy (FTIR) study. In addition, the synthesized AgNPs were tested for antioxidant and antibacterial activities. It showed good antioxidant activity as compared to butylated hydroxytoluene (BHT) and ascorbic acid as standard antioxidant. It could be concluded that H. isora root extract can be used efficiently in the production of potential antioxidant and antibacterial AgNPs for commercial application.

  1. Synthesis and Antibacterial Activities of Novel 4-Hydroxy-7-hydroxy- and 3-Carboxycoumarin Derivatives

    Directory of Open Access Journals (Sweden)

    Lin-Wen Lee

    2012-09-01

    Full Text Available Coumarin derivatives are used as fluorescent dyes and medicines. They also have some notable physiological effects, including the acute hepatoxicity and carcinogenicity of certain aflatoxins, the anticoagulant action of dicoumarol, and the antibiotic activity of novobicin and coumerymycin A1. Because the number of drug resistant strains is increasing at present, the synthesis of new antibacterial compounds is one of the critical methods for treating infectious diseases. Therefore, a series of coumarin-substituted derivatives, namely 4-hydroxy- and 7-hydroxycoumarins, and 3-carboxycoumarins were synthesized. 4-Hydroxycoumarin derivatives 4a–c underwent rearrangement reactions. Both 4- and 7-hydroxycoumarins were treated with activated aziridines which produced series of ring-opened products 7, 8, 10, and 11. 3-Carboxy-coumarin amide dimer derivatives 14–21 were prepared by reacting aliphatic alkylamines and alkyldiamines with PyBOP and DIEA. In this study, we use a new technique called modified micro-plate antibiotic susceptibility test method (MMAST, which is more convenient, more efficient, and more accurate than previous methods and only a small amount of the sample is required for the test. Some of the compounds were produced by reactions with acid anhydrides and demonstrated the ability to inhibit Gram-positive microorganisms. The dimer derivatives displayed lower antibacterial activities.

  2. Preparation of antibacterial textile using laser ablation method

    Science.gov (United States)

    Shahidi, Sheila; Rashidian, M.; Dorranian, D.

    2018-02-01

    A facile in situ laser ablation synthesis of Copper nanoparticles on cotton fabric is reported in this paper. This synthetic method is a laser ablation based fabrication of Cu nanoparticles on cotton fabric for improved performance and antibacterial activity. The treated cotton fabric was characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, UV-Visible spectroscopic techniques and antibacterial counting test. Very good antibacterial behavior of treated fabrics achieved. This fabric can be used as medical and industrial textiles.

  3. Synthesis, Antibacterial and Antifungal Activity of 4-Substituted-5-Aryl-1,2,4-Triazoles

    Directory of Open Access Journals (Sweden)

    Aleksandra Buzarovska

    2001-09-01

    Full Text Available A few 4-allyl/amino-5-aryl-1,2,4-triazoles were synthesized and tested for antibacterial and antifungal effects against Escherichia coli, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Aspergillus niger and Candida albicans. 4-Allyl-5-aryl-1,2,4-triazoles were obtained by the oxidative cyclization of the appropriate 1-substituted-4-allylthiosemicarbazides and 4-amino-5-aryl-1,2,4-triazoles were obtained by cyclization of the potassium salts of appropriately substituted dithiocarbazinic acids with hydrazine hydrate. The new synthesized compounds were characterized using IR, 1H- NMR, 13C-NMR and UV spectral data together with elemental analysis.

  4. Amine-functionalized, silver-exchanged zeolite NaY: Preparation, characterization and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Hanim, Siti Aishah Mohd; Malek, Nik Ahmad Nizam Nik, E-mail: niknizam@fbb.utm.my; Ibrahim, Zaharah

    2016-01-01

    Graphical abstract: - Highlights: • Functionalization of Ag-exchanged zeolite NaY with 3-aminopropyltriethoxysilane APTES (ZSA) as antibacterial agent. • Antibacterial assay of ZSA was performed against Escherichia coli ATCC11229 and Staphylococcus aureus ATCC6538. • Functionalization of Ag-exchanged zeolite NaY with APTES significantly increased the antibacterial agent. • Different mechanisms of bacterial death were suggested for each bacteria type by the functionalized Ag-exchanged zeolite NaY. - Abstract: Amine-functionalized, silver-exchanged zeolite NaY (ZSA) were prepared with three different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.20 and 0.40 M) and four different concentrations of silver ions (25%, 50%, 100% and 200% from zeolite cation exchange capacity (CEC)). The samples were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX), surface area analysis, thermogravimetric analysis (TGA) and zeta potential (ZP) analysis. The FTIR results indicated that the zeolite was functionalized by APTES and that the intensity of the peaks corresponding to APTES increased as the concentration of APTES used was increased. The antibacterial activities of the silver-exchanged zeolite NaY (ZS) and ZSA were studied against Escherichia coli ATCC11229 and Staphylococcus aureus ATCC6538 using the disc diffusion technique (DDT) and minimum inhibitory concentration (MIC). The antibacterial activity of ZSA increased with the increase in APTES on ZS, and E. coli was more susceptible towards the sample compared to S. aureus. The FESEM micrographs of the bacteria after contact with the ZSA suggested different mechanisms of bacterial death for these two bacteria due to exposure to the studied sample. The functionalization of ZS with APTES improved the antibacterial activity of the silver-zeolite, depending on the concentration of silver

  5. Silver/poly (lactic acid nanocomposites: preparation, characterization, and antibacterial activity

    Directory of Open Access Journals (Sweden)

    Kamyar Shameli

    2010-08-01

    Full Text Available Kamyar Shameli1, Mansor Bin Ahmad1, Wan Md Zin Wan Yunus1, Nor Azowa Ibrahim1, Russly Abdul Rahman2, Maryam Jokar2,3, Majid Darroudi41Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia; 2Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, Malaysia; 3Department of Food Technology, Islamic Azad University, Damghan Branch, Damghan, Iran; 4Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology (ITMA, Universiti Putra Malaysia, Selangor, MalaysiaAbstract: In this study, antibacterial characteristic of silver/poly (lactic acid nanocomposite (Ag/PLA-NC films was investigated, while silver nanoparticles (Ag-NPs were synthesized into biodegradable PLA via chemical reduction method in diphase solvent. Silver nitrate and sodium borohydride were respectively used as a silver precursor and reducing agent in the PLA, which acted as a polymeric matrix and stabilizer. Meanwhile, the properties of Ag/PLA-NCs were studied as a function of the Ag-NP weight percentages (8, 16, and 32 wt% respectively, in relation to the use of PLA. The morphology of the Ag/PLA-NC films and the distribution of the Ag-NPs were also characterized. The silver ions released from the Ag/PLA-NC films and their antibacterial activities were scrutinized. The antibacterial activities of the Ag/PLA-NC films were examined against Gram-negative bacteria (Escherichia coli and Vibrio parahaemolyticus and Gram-positive bacteria (Staphylococcus aureus by diffusion method using Muller–Hinton agar. The results indicated that Ag/PLA-NC films possessed a strong antibacterial activity with the increase in the percentage of Ag-NPs in the PLA. Thus, Ag/PLA-NC films can be used as an antibacterial scaffold for tissue engineering and medical application.Keywords: antibacterial activity, silver nanoparticles, biodegradable, poly (lactic acid, Muller–Hinton agar.

  6. Lactobacillus species mediated synthesis of silver nanoparticles and their antibacterial activity against opportunistic pathogens in vitro

    Directory of Open Access Journals (Sweden)

    Inna Garmasheva

    2016-12-01

    Results: The capsular layers of Lactobacillus strains contained heteropolysaccharides that were composed mostly of glucose, mannose, galactose and rhamnose in a different molar ratio. It was found that Ag NPs with large size (30.65 ± 5.81 nm obtained from L. acidophilus 58p were more active against S. epidermidis, E. coli, K. pneumonia, S. flexneri and S. sonnei compared with Ag NPs from L. plantarum 92T (19.92 ± 3.4 nm. Conclusion: The size and antibacterial activities of Ag NPs were strain-dependent and such characteristics may be due to the capsular biopolymer composition of Lactobacillus strains used for Ag NPs synthesis.

  7. RETRACTED: Synthesis of silver nanoparticles using A. indicum leaf extract and their antibacterial activity

    Science.gov (United States)

    Ashokkumar, S.; Ravi, S.; Kathiravan, V.; Velmurugan, S.

    2015-01-01

    This article has been retracted: please see Elsevier Policy on Article Withdrawal. This article has been retracted at the request of the Editor. The article contains an image (Fig. 7D) which originates from an image previously published in the article "Rapid biological synthesis of silver nanoparticles using Leucas martinicensis leaf extract for catalytic and antibacterial activity" by S. Ashokkumar et al. in Environ Sci Pollut Res (2014) 21:11439-11446, DOI 10.1007/s11356-014-3012-7 (Fig. 7D), despite being attributed to different samples. Furthermore, Figures 8A and 8B show identical images despite the claim of different samples. Finally, Figure 3 displays duplicated data despite being described as different samples. The scientific community takes a very strong view on this scientific misbehavior and apologies are offered to readers of the journal that this was not detected during the submission process.

  8. Green synthesis of silver nanoparticles by Ricinus communis var. carmencita leaf extract and its antibacterial study

    Science.gov (United States)

    Ojha, Sunita; Sett, Arghya; Bora, Utpal

    2017-09-01

    In this study, we report synthesis of silver nanoparticles (RcAgNPs) from silver nitrate solution using methanolic leaf extract of Ricinus communis var. carmencita. The polyphenols present in the leaves reduce Ag++ ions to Ag0 followed by a color change. Silver nanoparticle formation was ensured by surface plasmon resonance between 400 nm to 500 nm. Crystallinity of the synthesized nanoparticles was confirmed by UHRTEM, SAED and XRD analysis. The capping of phytochemicals and thermal stability of RcAgNPs were assessed by FTIR spectra and TGA analysis, respectively. It also showed antibacterial activity against both gram positive and gram negative strains. RcAgNPs were non-toxic against normal cell line (mouse fibroblast cell line L929) at lower concentrations (80 µg ml-1).

  9. The Nucleotide Synthesis Enzyme CAD Inhibits NOD2 Antibacterial Function in Human Intestinal Epithelial Cells

    Science.gov (United States)

    Richmond, Amy L.; Kabi, Amrita; Homer, Craig R.; García, Noemí Marina; Nickerson, Kourtney P.; NesvizhskiI, Alexey I.; Sreekumar, Arun; Chinnaiyan, Arul M.; Nuñez, Gabriel; McDonald, Christine

    2013-01-01

    BACKGROUND & AIMS Polymorphisms that reduce the function of nucleotide-binding oligomerization domain (NOD)2, a bacterial sensor, have been associated with Crohn’s disease (CD). No proteins that regulate NOD2 activity have been identified as selective pharmacologic targets. We sought to discover regulators of NOD2 that might be pharmacologic targets for CD therapies. METHODS Carbamoyl phosphate synthetase/ aspartate transcarbamylase/dihydroorotase (CAD) is an enzyme required for de novo pyrimidine nucleotide synthesis; it was identified as a NOD2-interacting protein by immunoprecipitation-coupled mass spectrometry. CAD expression was assessed in colon tissues from individuals with and without inflammatory bowel disease by immunohistochemistry. The interaction between CAD and NOD2 was assessed in human HCT116 intestinal epithelial cells by immunoprecipitation, immunoblot, reporter gene, and gentamicin protection assays. We also analyzed human cell lines that express variants of NOD2 and the effects of RNA interference, overexpression and CAD inhibitors. RESULTS CAD was identified as a NOD2-interacting protein expressed at increased levels in the intestinal epithelium of patients with CD compared with controls. Overexpression of CAD inhibited NOD2-dependent activation of nuclear factor κB and p38 mitogen-activated protein kinase, as well as intracellular killing of Salmonella. Reduction of CAD expression or administration of CAD inhibitors increased NOD2-dependent signaling and antibacterial functions of NOD2 variants that are and are not associated with CD. CONCLUSIONS The nucleotide synthesis enzyme CAD is a negative regulator of NOD2. The antibacterial function of NOD2 variants that have been associated with CD increased in response to pharmacologic inhibition of CAD. CAD is a potential therapeutic target for CD. PMID:22387394

  10. Synthesis and spectroscopic characterization of mononuclear/binuclear organotin(IV complexes with 1H-1,2,4-triazole-3-thiol: Comparative studies of their antibacterial/antifungal potencies

    Directory of Open Access Journals (Sweden)

    Parveen Bushra

    2015-01-01

    Full Text Available A series of di and triorganotin(IV complexes of the general formula, R2(ClSnL (R= Me: 1; Bu: 2 and R3SnL (R = Bu: 3; Ph: 4 have been synthesized by refluxing equivalent molar ratios of orgnotin(IV chlorides (R2SnCl2/R3SnCl with the 1H-1,2,4-Triazole-3-thiol (LH in dry methanol. The synthesized complexes (1-4 were further treated with CS2 and R2SnCl2/R3SnCl in 1:1:1 molar ratio to yield the homobimetallic complexes of the types R2(ClSnLCS2Sn(ClR2 (R = Me: 5; Bu: 6 and R3SnLCS2SnR3 (R = Bu: 7; Ph: 8. The ligand and the complexes have been characterized by elemental microanalysis (CHNS, FT-IR and multinuclear NMR (1H&13C, and electron ionization mass spectrometry. IR data demonstrates that the dithiocarbamate donor site of the ligand acts in a bidentate manner and there isa trigonal bipyramidal geometry around Sn(IV in solid state. 1H and 13C NMR data supports the tetrahedralgeometry with thiol donor sites of the ligand while tetra and penta coordinated environments around dithiocarboxylate bound tin(IV in solution state. Mass spectrometric data supported well the structures of the synthesized complexes. The homobimetallic derivatives were found more active than mononuclear organotin(IV compounds and free ligand against various strains of bacteria and fungus.

  11. Quinoline derivatives bearing pyrazole moiety: Synthesis and biological evaluation as possible antibacterial and antifungal agents.

    Science.gov (United States)

    El Shehry, Mohamed F; Ghorab, Mostafa M; Abbas, Samir Y; Fayed, Eman A; Shedid, Said A; Ammar, Yousry A

    2018-01-01

    In an attempt for development of new antimicrobial agents, three series of quinoline derivatives bearing pyrazole moiety have been synthesized. The first series was synthesized through the synthesis of 4-(quinolin-2-yloxy)benzaldehyde and 4-(quinolin-2-yloxy)acetophenone and then treatment with ketone or aldehyde derivatives to afford the corresponding chalcones. Cyclization of the latter chalcones with hydrazine derivatives led to the formation of new pyrazoline derivatives. The second series was synthesized via the synthesis of 2-hydrazinylquinoline and then treatment with formylpyrazoles to afford the corresponding hydrazonyl pyrazole derivatives. The third series was synthesized through the treatment of 2-hydrazinylquinoline with ethoxyethylidene, dithioacetal and arylidene derivatives to afford the corresponding pyrazole derivatives. The synthesized compounds were evaluated for their expected antibacterial and antifungal activities; where, the majority of these compounds showed potent antibacterial and antifungal activities against the tested strains of bacteria and fungi. Pyrazole derivative 13b showed better results when compared with the reference drugs as revealed from their MIC values (0.12-0.98 μg/mL). The pyrazole derivative 13b showed fourfold potency of gentamycin in inhibiting the growth of S. flexneri (MIC 0.12 μg/mL). Also, compound 13b showed fourfold potency of amphotericin B in inhibiting the growth of A. clavatus (MIC 0.49 μg/mL) and C. albicans (MIC 0.12 μg/mL), respectively. The same compound showed twofold potency of gentamycin in inhibiting the growth of P. vulgaris (MIC 0.98 μg/mL), equipotent to the ampicillin and amphotericin B in inhibiting the growth of S. epidermidis (MIC 0.49 μg/mL), A. fumigatus (MIC 0.98 μg/mL), respectively. Thus, these studies suggest that quinoline derivatives bearing pyrazole moiety are interesting scaffolds for the development of novel antibacterial and antifungal agents. Copyright © 2017

  12. Characterization of antibacterial and adhesion properties of chitosan-modified glass ionomer cement.

    Science.gov (United States)

    Ibrahim, Marrwa A; Neo, Jennifer; Esguerra, Roxanna J; Fawzy, Amr S

    2015-10-01

    The aim is to investigate the effect of modifying the liquid phase of a conventional glass ionomer restorative material with different chitosan volume contents on the antibacterial properties and adhesion to dentin. The liquids of commercially available restorative glass ionomer cements (GIC) were modified with chitosan (CH) solutions at different volume contents (5%, 10%, 25%, and 50%). The GIC powders were mixed with the unmodified and the CH-modified liquids at the desired powder/liquid (P/L) ratio. For the characterization of the antibacterial properties, Streptococcus mutans biofilms were formed on GIC discs and characterized by scanning electron microscope (SEM), confocal microscopy, colony forming unit (CFU) count, and cell viability assay (MTS). The unmodified and CH-modified GICs were bonded to dentin surfaces and the micro-tensile bond strength (µTBs) was evaluated and the interface was investigated by SEM. Modification with CH solutions enhanced the antibacterial properties against S. mutans in terms of resistance to biofilm formation, CFU count, and MTS assay. Generally, significant improvement in the antibacterial properties was found with the increase in the CH volume content. Modification with 25% and 50% CH adversely affected the µTBs with predominant cohesive failure in the GIC. However, no difference was found between the control and the 5% and 10% CH-modified specimens. Incorporation of acidic solutions of chitosan in the polyacrylic acid liquid of GIC at v/v ratios of 5-10% improved the antibacterial properties of conventional glass ionomer cement against S. mutans without adversely affecting its bonding to dentin surface. © The Author(s) 2015.

  13. Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

    Directory of Open Access Journals (Sweden)

    Thekkae Padil VV

    2013-02-01

    Full Text Available Vinod Vellora Thekkae Padil, Miroslav ČerníkLaboratory of Chemical Remediation Processes, Institute for Nanomaterials, Advanced Technology and Innovation, Technical University of Liberec, Studentská 1402/2, Liberec, Czech RepublicBackground: Copper oxide (CuO nanoparticles have attracted huge attention due to catalytic, electric, optical, photonic, textile, nanofluid, and antibacterial activity depending on the size, shape, and neighboring medium. In the present paper, we synthesized CuO nanoparticles using gum karaya, a natural nontoxic hydrocolloid, by green technology and explored its potential antibacterial application.Methods: The CuO nanoparticles were synthesized by a colloid-thermal synthesis process. The mixture contained various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and 3 mM and gum karaya (10 mg/mL and was kept at 75°C at 250 rpm for 1 hour in an orbital shaker. The synthesized CuO was purified and dried to obtain different sizes of the CuO nanoparticles. The well diffusion method was used to study the antibacterial activity of the synthesized CuO nanoparticles. The zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration were determined by the broth microdilution method recommended by the Clinical and Laboratory Standards Institute.Results: Scanning electron microscopy analysis showed CuO nanoparticles evenly distributed on the surface of the gum matrix. X-ray diffraction of the synthesized nanoparticles indicates the formation of single-phase CuO with a monoclinic structure. The Fourier transform infrared spectroscopy peak at 525 cm−1 should be a stretching of CuO, which matches up to the B2u mode. The peaks at 525 cm−1 and 580 cm−1 indicated the formation of CuO nanostructure. Transmission electron microscope analyses revealed CuO nanoparticles of 4.8 ± 1.6 nm, 5.5 ± 2.5 nm, and 7.8 ± 2.3 nm sizes were synthesized with various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and

  14. Fabrication and characterization of electrospun poly(e-caprolactone) fibrous membrane with antibacterial functionality

    Science.gov (United States)

    Cerkez, Idris; Sezer, Ayse; Bhullar, Sukhwinder K.

    2017-02-01

    This research study is mainly targeted on fabrication and characterization of antibacterial poly(e-caprolactone) (PCL) based fibrous membrane containing silver chloride particles. Micro/nano fibres were produced by electrospinning and characterized with TGA, DSC, SEM and mechanical analysis. It was found that addition of silver particles slightly reduced onset of thermal degradation and increased crystallization temperature of neat PCL. Silver-loaded samples exhibited higher tensile stress and lower strain revealing that the particles behaved as reinforcing agent. Moreover, addition of silver chloride resulted in beaded surface texture and formation of finer fibres as opposed to the neat. Antibacterial properties were tested against Gram-negative and Gram-positive bacteria and remarkable biocidal functionalities were obtained with about six logs reduction of Staphylococcus aureus and Escherichia coli O157:H7.

  15. [Preparation and characterization of activated carbon-silver composite with antibacterial behavior via vacuum impregnation method].

    Science.gov (United States)

    Wang, Zi-Qiang; Liu, Shou-Xin

    2011-01-01

    Activated carbon-silver composite (Ag/AC) for antibacterial performance by controlling silver release was prepared by silver acetate vacuum impregnation method. The antibacterial activity towards E. coil and resistance of water erosion was investigated through distilled water. Surface area and porosity analyzer, Scanning electron spectroscopy (SEM) and X-ray diffraction (XRD) were used to characterize the surface morphology and pore properties. The results show that Ag0 was deposited on AC symmetrically. The content of silver supported and particle size were increased by the increasing of the concentration of CH3 COOAg, while specific surface area, total pore volume and average pore size were decreased. Ag/AC prepared with silver content of 0.97% which killed 10(7) CFU/mL concentration of E. coil in 120 min exhibited the similar antibacterial activity for E. coil with that prepared by traditional impregnation method. However, the silver loss of the Ag/AC prepared with silver content of 0.97% was 37.6%, showing much higher resistance to water erosion. High antibacterial activity and control silver release can be simultaneously realized by the silver acetate vacuum impregnation method.

  16. Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes Micrococcus yunnanensis

    Directory of Open Access Journals (Sweden)

    Ravi Ranjan

    2017-10-01

    Full Text Available Endophytic actinomycetes are considered as one of the relatively unexplored potential sources in search of antibiotic producer against antibiotic resistant pathogens. A potent strain isolated from Catharanthus roseus that displays antibacterial potential against antibiotic resistant human pathogen Staphylococcus aureus was characterized and designated as Micrococcus yunnanensis strain rsk5. Rsk5 is capable of producing optimum antibacterial metabolites on starch casein medium at 30 °C, pH 5 and 2% NaCl condition. The crude antibacterial agent was extracted from fermentation broth by ethyl acetate and separated by TLC using chloroform-methanol (24:1, v/v solvent system with Rf value of 0.26. It was partially purified by flash chromatography, followed by HPLC and analyzed by ultraviolet visible spectrophotometer to get absorption maxima at 208.4 nm. The ESI-MS spectra showed molecular ion peaks at m/z 472.4 [M-H], which does not match with any known antibacterial compound.

  17. Spectroscopic Characterization and Nanosafety of Ag-Modified Antibacterial Leather and Leatherette.

    Science.gov (United States)

    Sportelli, Maria Chiara; Picca, Rosaria Anna; Paladini, Federica; Mangone, Annarosa; Giannossa, Lorena Carla; Franco, Cinzia Di; Gallo, Anna Lucia; Valentini, Antonio; Sannino, Alessandro; Pollini, Mauro; Cioffi, Nicola

    2017-07-29

    The development of antibacterial coatings is of great interest from both industry and the consumer's point of view. In this study, we characterized tanned leather and polyurethane leatherette, typically employed in the automotive and footwear industries, which were modified by photo-deposition of antibacterial silver nanoparticles (AgNPs). Material surface chemical composition was investigated in detail by X-ray photoelectron spectroscopy (XPS). The material's antibacterial capability was checked against Escherichia coli and Staphylococcus aureus , as representative microorganisms in cross transmissions. Due to the presence of silver in a nanostructured form, nanosafety issues were considered, as well. Ionic release in contact media, as well as whole nanoparticle release from treated materials, were quantitatively evaluated, thus providing specific information on potential product nanotoxicity, which was further investigated through cytocompatibility MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, also after surface abrasion of the materials. The proved negligible nanoparticle release, as well as the controlled release of antibacterial ions, shed light on the materials' potentialities, in terms of both high activity and safety.

  18. Spectroscopic Characterization and Nanosafety of Ag-Modified Antibacterial Leather and Leatherette

    Directory of Open Access Journals (Sweden)

    Maria Chiara Sportelli

    2017-07-01

    Full Text Available The development of antibacterial coatings is of great interest from both industry and the consumer’s point of view. In this study, we characterized tanned leather and polyurethane leatherette, typically employed in the automotive and footwear industries, which were modified by photo-deposition of antibacterial silver nanoparticles (AgNPs. Material surface chemical composition was investigated in detail by X-ray photoelectron spectroscopy (XPS. The material’s antibacterial capability was checked against Escherichia coli and Staphylococcus aureus, as representative microorganisms in cross transmissions. Due to the presence of silver in a nanostructured form, nanosafety issues were considered, as well. Ionic release in contact media, as well as whole nanoparticle release from treated materials, were quantitatively evaluated, thus providing specific information on potential product nanotoxicity, which was further investigated through cytocompatibility MTT (3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays, also after surface abrasion of the materials. The proved negligible nanoparticle release, as well as the controlled release of antibacterial ions, shed light on the materials’ potentialities, in terms of both high activity and safety.

  19. Physicochemical characterization and antibacterial activity of Rajata Bhasma and silver nanoparticle

    OpenAIRE

    Sharma, Revati; Bhatt, Ahsan; Thakur, Mansee

    2016-01-01

    Introduction: Bhasma, an Ayurvedic metallo-mineral preparation, is claimed to be biologically produced nanoparticles. Rajata (silver) is a noble metal known for its antimicrobial activity. Rajata Bhasma (RB) is expected to be composed of nanoparticles. With all these facts in place, this study was conducted to evaluate RB for the presence of silver nanoparticle (SNP) and its antimicrobial effect. Aim: The aim of this study is to analyze the physicochemical characterization, antibacterial acti...

  20. Preparation and characterization of BC/PAM-AgNPs nanocomposites for antibacterial applications.

    Science.gov (United States)

    Yang, Guang; Wang, Caixia; Hong, Feng; Yang, Xuexia; Cao, Zhangjun

    2015-01-22

    In this work, a bacterial cellulose/polyacrylamide (BC/PAM) double network composite was prepared to act as the template for in situ synthesis of silver nanoparticles (AgNPs). Effects of reaction conditions of the BC/PAM composite were investigated on its microstructure, mechanical properties and thermal stabilities. Both the BC/PAM composite and pure BC were utilized to prepare the corresponding silver impregnated nanocomposites, i.e., BC/PAM-AgNPs and BC-AgNPs, by an environmental friendly method, UV irradiation. The influences of the templates were investigated on the AgNPs formation and the antibacterial activities of the nanocomposites by both the zone of inhibition and dynamic shake flask methods. It was shown that the BC/PAM composite displayed a denser microstructure and higher thermal stabilities than pure BC. The BC/PAM-AgNPs nanocomposite exhibited a bigger particle size and lower mass content of AgNPs than the BC-AgNPs one. For the antibacterial test, two nanocomposites exhibited a close antibacterial effect, with a high log reduction above 3 and killing ratio above 99.9%, respectively. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. SYNTHESIS, CHARACTERIZATION AND DENSITY FUNCTIONAL ...

    African Journals Online (AJOL)

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    KEY WORDS: Monoacyl aniline, Synthesis, Density functional theory, Rotation barrier. INTRODUCTION ... on the electron density in the phenyl ring and the respective accelerating and decelerating effects on the rate of ... compounds were determined using Nujol mulls and of liquids either in dichloromethane or chloroform ...

  2. SYNTHESIS AND CHARACTERIZATION OF FUNCTIONALIZED ...

    African Journals Online (AJOL)

    2016-07-30

    E-mail: b_mohtat@yahoo.com. This work is licensed under the Creative Commons ... Department of Chemistry, Islamic Azad University, Karaj Branch, Karaj, Iran. (Received July 30, 2016; revised ..... Chem. 2013, 9, 2846-2851. 7. Mohamed, K.S.; Soliman, M.A.; El-Remaily, M.A.A.; Abdel-Ghany, H. Eco-friendly synthesis of ...

  3. Green synthesis of silver nanoparticles using green tea leaves: Experimental study on the morphological, rheological and antibacterial behaviour

    Science.gov (United States)

    Nakhjavani, Maryam; Nikkhah, V.; Sarafraz, M. M.; Shoja, Saeed; Sarafraz, Marzieh

    2017-10-01

    In this paper, silver nanoparticles are produced via green synthesis method using green tea leaves. The introduced method is cost-effective and available, which provides condition to manipulate and control the average nanoparticle size. The produced particles were characterized using x-ray diffraction, scanning electron microscopic images, UV visualization, digital light scattering, zeta potential measurement and thermal conductivity measurement. Results demonstrated that the produced samples of silver nanoparticles are pure in structure (based on the x-ray diffraction test), almost identical in terms of morphology (spherical and to some extent cubic) and show longer stability when dispersed in deionized water. The UV-visualization showed a peak in 450 nm, which is in accordance with the previous studies reported in the literature. Results also showed that small particles have higher thermal and antimicrobial performance. As green tea leaves are used for extracting the silver nanoparticles, the method is eco-friendly. The thermal behaviour of silver nanoparticle was also analysed by dispersing the nanoparticles inside the deionized water. Results showed that thermal conductivity of the silver nano-fluid is higher than that of obtained for the deionized water. Activity of Ag nanoparticles against some bacteria was also examined to find the suitable antibacterial application for the produced particles.

  4. Synthesis, characterization and in vitro antibacterial activity of novel ...

    Indian Academy of Sciences (India)

    MARZIEH ABBASI

    bDepartment of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of. Medical Sciences, Isfahan, Islamic ... recent years due to their wide range of biological proper- ties. Sulfonamides derivatives are ...... tration of tested samples (μg/mL) giving 50% inhibition were calculated from the ...

  5. Silver Colloid Nanoparticles: Synthesis, Characterization, and Their Antibacterial Activity

    Czech Academy of Sciences Publication Activity Database

    Panáček, A.; Kvítek, L.; Prucek, R.; Kolář, M.; Večeřová, R.; Pizúrová, Naděžda; Sharma, V. K.; Nevěčná, T.; Zbořil, R.

    2006-01-01

    Roč. 110, č. 33 (2006), s. 16248-16253 ISSN 1520-6106 R&D Projects: GA MŠk(CZ) 1M0512 Institutional research plan: CEZ:AV0Z20410507 Keywords : silver colloid nanoparticles * antimicrobial and bactericidal assays * particle size Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 4.115, year: 2006

  6. synthesis, characterization and antibacterial studies of some metal ...

    African Journals Online (AJOL)

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    region due to L → L* transitions, only. As expected, the electronic spectra of these complexes do not furnish any useful information on their stereochemistry. However, on the basis of analytical, conductance and infrared spectral data, a tetrahedral geometry is proposed for the. Zn(II), Cd(II) and Hg(II) complexes. ESR and 1H ...

  7. Synthesis, Characterization and Antibacterial Activity of New 1,2 ...

    African Journals Online (AJOL)

    NICO

    -II diffractometer with graphite-monochromated MoKa radiation. An empiri- cal absorption correction with the PLATON program20 was performed. The structure was solved by direct methods and refined with full-matrix least-squares on F2 using ...

  8. Synthesis, characterization and antibacterial activity of new salicylhydrazide containing azopyrazoles

    Directory of Open Access Journals (Sweden)

    Bhupendra P. Patel

    2011-03-01

    Full Text Available Various ethyl-2-substituted phenyl hydrazono-3-oxobutyrate (2a-h condensation with 2-hydroxy benzoic acid hydrazide (3 to afford 1-(2-hydroxybenzoyl-3-methyl-4-(2- substituted phenyl hydrazono-1H-pyrazol-5(4H-one (4a-h. The structures of all these compounds (4a-h were recognized on basis of analytical and spectral data. The newly synthesized compounds were evaluated for their antimicrobial activity against various bacteria and fungi.

  9. Synthesis of magnetic graphene oxide–TiO{sub 2} and their antibacterial properties under solar irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Ying-Na [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Ou, Xiao-Ming [China National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014 (China); Zeng, Guang-Ming [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Gong, Ji-Lai, E-mail: jilaigong@gmail.com [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China); Deng, Can-Hui; Jiang, Yan; Liang, Jie; Yuan, Gang-Qiang; Liu, Hong-Yu; He, Xun [College of Environmental Science and Engineering, Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha 410082 (China)

    2015-07-15

    Highlights: • Magnetic graphene oxide–TiO{sub 2} (MGO–TiO{sub 2}) composites were synthesized. • MGO–TiO{sub 2} had excellent antibacterial activity toward Escherichia coli. • MGO–TiO{sub 2} could effectively and rapidly separate from aqueous solution. • Carbonates and phosphates significantly reduced the bacterial survival rate. - Abstract: Titanium dioxide (TiO{sub 2}) has been intensively researched and increasingly used as antibacterial agent, but it suffers from separation inconvenience. Its effective removal from water after reaction while maintaining its high antibacterial activity becomes necessary. In this work, it was the first time the magnetic graphene oxide–TiO{sub 2} (MGO–TiO{sub 2}) composites were prepared through a simple synthesis method. The results indicated that MGO–TiO{sub 2} exhibited a good antibacterial activity against Escherichia coli. MGO–TiO{sub 2} was found to almost completely inactivate the E. coli within 30 min under solar irradiation. The effect of inorganic ions present in E. coli suspension was also evaluated. Compared with other ions, HCO{sub 3}{sup −} and HPO{sub 4}{sup 2−} had a greater influence on the antibacterial property.

  10. Rapid biosynthesis and characterization of silver nanoparticles: an assessment of antibacterial and antimycotic activity

    Science.gov (United States)

    Kanawaria, Sajjan Kumar; Sankhla, Aryan; Jatav, Pradeep Kumar; Yadav, Raghvendra Singh; Verma, Kumar Sambhav; Velraj, Parthiban; Kachhwaha, Sumita; Kothari, Shanker Lal

    2018-04-01

    Bioassisted synthesis provides a facile, convenient, and promising approach to produce many inorganic nanostructures. Herein, we report a rapid biosynthesis of silver nanoparticles (AgNPs) using Thuja occidentalis (L.) leaf extract with an emphasis on their antibacterial and antimycotic activity. Interestingly, the synthesis of AgNPs was completed in a short duration of 35-40 min. The electron micrographs showed AgNPs with particles 15 mm. The antimycotic activity against Aspergillus niger, Fusarium spp., and Alternaria alternata species increased monotonically with nanoparticle concentration in the growth media. A 10 ppm solution of AgNP was detrimental to fungal growth. Thus, the technique provides an avenue to synthesize antibiotic AgNPs without use of other external agents.

  11. Piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural plant pathogens.

    Science.gov (United States)

    Paulkumar, Kanniah; Gnanajobitha, Gnanadhas; Vanaja, Mahendran; Rajeshkumar, Shanmugam; Malarkodi, Chelladurai; Pandian, Kannaiyan; Annadurai, Gurusamy

    2014-01-01

    Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7-50 nm and 9-30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology.

  12. Piper nigrum Leaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens

    Science.gov (United States)

    Paulkumar, Kanniah; Gnanajobitha, Gnanadhas; Vanaja, Mahendran; Rajeshkumar, Shanmugam; Malarkodi, Chelladurai; Pandian, Kannaiyan; Annadurai, Gurusamy

    2014-01-01

    Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology. PMID:24558336

  13. Piper nigrum Leaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens

    Directory of Open Access Journals (Sweden)

    Kanniah Paulkumar

    2014-01-01

    Full Text Available Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD, scanning electron microscope (SEM, transmission electron microscope (TEM, energy dispersive X-ray analysis (EDAX, and Fourier Transform Infrared Spectroscopy (FTIR. The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology.

  14. Mycosynthesis, characterization and antibacterial properties of AgNPs against multidrug resistant (MDR bacterial pathogens of female infertility cases

    Directory of Open Access Journals (Sweden)

    Ponnusamy Manogaran Gopinath

    2015-04-01

    Full Text Available Recently, biosynthesis of silver nanoparticles using bacteria, fungus and plants has emerged as a simple and viable alternative to more complex physical and chemical synthetic procedures. The present investigation explains rapid and extracellular synthesis of silver nanoparticles using fungus Fusarium oxysporum NGD and characterization of the synthesized silver nanoparticles using UV-Vis spectroscopy, scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction analysis. The size range of the synthesized silver nanoparticles was around 16.3–70 nm. The FTIR studies showed major peaks of proteins involved in the synthesis of silver nanoparticles. Further, antibacterial effect of the silver nanoparticles against multidrug resistant pathogens Enterobacter sp. ANT 02 [HM803168], Pseudomonas aeruginosa ANT 04 [HM803170], Klebsiella pneumoniae ANT 03 [HM803169] and Escherichia coli ANT 01 [HM803167] was tested using turbidometric assay at 10, 20, 30, 40 μg AgNPs/ml alone and in combination with ampicillin using agar well diffusion assay. All the resistant bacteria were found to be susceptible to the antibiotic in the presence of the silver nanoparticles.

  15. Preparation and characterization of antibacterial orthodontic resin containing silver nanoparticles

    Science.gov (United States)

    Lee, Sang Jin; Heo, Min; Lee, Donghyun; Han, Seungheui; Moon, Ji-Hoi; Lim, Ho-Nam; Kwon, Il Keun

    2018-02-01

    In this study, we developed a hybrid dental resin containing silver nanoparticle (AgNPs) to eliminate periodontal disease causing bacteria such as streptococcus mutans (S. mutans) and streptococcus sobrinus (S. sobrinus). The silver nanoparticles enables the resin to prevent oral pathogen growth during orthodontic therapy. First, AgNPs were directly synthesized in dimethylformamide (DMF) solvent with a capping agent. Second, pure orthodontic primer was mixed with the synthesized AgNPs solvent-slurry followed by photocuring. The resultant material was characterized by physicochemical characterization. Finally, an in vitro antimicrobial test was carried out. The results showed that the AgNPs were fully synthesized and clearly embedded in dental resin. In the bacterial test, the dental resin containing AgNPs showed potent antimicrobial activity against two kinds of bacteria. In conclusion, our methodology may allow for the generation of a wide range of dental resin and composite products which inhibit periodontitis causing bacteria.

  16. A Convenient Ultraviolet Irradiation Technique for Synthesis of Antibacterial Ag-Pal Nanocomposite

    Science.gov (United States)

    Han, Shuai; Zhang, He; Kang, Lianwei; Li, Xiaoliang; Zhang, Chong; Dong, Yongjie; Qin, Shenjun

    2016-09-01

    In the present work, palygorskite (Pal) was initially subjected to an ion-exchange reaction with silver ions (Pal-Ag+). Subsequently, Ag-Pal nanocomposites were assembled by a convenient ultraviolet irradiation technique, using carbon dots (CDs) derived from wool fiber as the reducing agent. The obtained nanocomposites were characterized by powder X-ray diffraction (XRD), ultraviolet-visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy. The XRD patterns and UV-vis absorption spectra confirmed the formation of the Ag nanoparticles (NPs). Meanwhile, the TEM images showed that the Ag NPs, which exhibited sizes in the range of 3-7 nm, were located on the surface of the Pal nanofiber structures. Furthermore, the antibacterial activity of the nanocomposites was evaluated against Gram-positive ( Staphylococcus aureus) and Gram-negative ( Escherichia coli) bacteria by applying the disc diffusion method and minimum inhibitory concentration test. Owing to their good antibacterial properties, the Ag-Pal nanocomposites are considered to be a promising bactericide with great potential applications.

  17. Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities

    Science.gov (United States)

    Manikandan, Ramar; Manikandan, Beulaja; Raman, Thiagarajan; Arunagirinathan, Koodalingam; Prabhu, Narayanan Marimuthu; Jothi Basu, Muthuramalingam; Perumal, Muthulakshmi; Palanisamy, Subramanian; Munusamy, Arumugam

    2015-03-01

    The present study was aimed at biosynthesis of silver nanoparticles (AgNPs) using ethanolic extract of rose (Rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line HCT 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. The biologically synthesized AgNPs were also characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The characterized AgNPs showed an effective antibacterial activity against Gram negative (Escherichia coli, Klebsiella pneumoniae) than Gram positive (Streptococcus mutans, Enterococcus faecalis) bacteria. MTT assay, analysis of nuclear morphology, mRNA expression of Bcl-2, Bax and protein expression of caspase 3 as well as 9, indicated potential anticancer activity. In addition, green synthesized AgNPs also attenuated cytotoxicity, nuclear morphology and free radical generation (O2- and NO) by rat peritoneal macrophages in vitro. The results of our study show the potential green synthesis of silver nanoparticles in mitigating their toxicity while retaining their antibacterial activities.

  18. Nano polyamidoamine-G7 dendrimer synthesis and assessment the antibacterial effect in vitro

    Directory of Open Access Journals (Sweden)

    Mitra Gholami

    2016-04-01

    Full Text Available Background: Nano scale dendrimers are macromolecules synthetic which frequently used in medical and health field. Because traditional antibiotics inevitably induce bacterial resistance, which is responsible for many treatment failures, there is an urgent need to develop novel antibiotic drugs. This study was aimed to examine Synthesis and the antibacterial effect of NanoPolyamidoamine-G7 (NPAMAM-G7 dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus. Methods: In this experimental study that has been conducted in June 2015 in the Laboratory of Microbiology, Iran University of Medical Science, NPAMAM-G7 dendrimers was synthesized by Tomalia’s divergent growth approach. The antibacterial effects of NPAMAM-G7 dendrimer were studied by disc diffusion and micro-dilution method. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC against gram-positive and gram-negative bacteria were determined according to Clinical and Laboratory Standards Institute (CLSI guideline. Standard discs were prepared using different concentrations of dendrimer on Mueller-Hinton agar plates. Results: Zone of inhibition in concentration 25 μg/ml of NPAMAM-G7 dendrimers for Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus were 26, 38, 36, 22 and 25 mm, respectively. Regarding the zone of inhibition in gram negative bacteria with gram positive ones was P= 0.16 and was not significant difference. The MIC for Salmonella Typhi was 0.025, for Proteus Mirabilis, Bacillus Subtilis, Staphylococcus Aureus and Escherichia Coli was 0.25 μg/ml. The MBC for Salmonella Typhi was 25μg/ml, for Proteus Mirabilis and Bacillus Subtilis was 50 μg/ml and for Escherichia Coli and Staphylococcus Aureus was 100 μg/ml. The least of sensitivity against NPAMAM-G7 related to Escherichia Coli and Staphylococcus Aureus and the most of sensitivity related to

  19. Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nanoparticles using aqueous garlic (Allium sativum) extract and their antibacterial potential

    Energy Technology Data Exchange (ETDEWEB)

    Rastogi, Lori [National Center for Chemical Characterization of Materials, Bhabha Atomic Research Centre, ECIL-PO, Hyderabad 500 062 (India); Arunachalam, J., E-mail: aruncccm@rediffmail.com [National Center for Chemical Characterization of Materials, Bhabha Atomic Research Centre, ECIL-PO, Hyderabad 500 062 (India)

    2011-09-15

    Highlights: {yields} We report green synthetic route for the production crystalline silver nanoparticles using garlic as both reducing and stabilizing agent. {yields} Synthesis has been achieved by exposing the solution mixture of [Ag(NH{sub 3}){sub 2}]{sup +} and aqueous garlic extract under sunlight. {yields} Role of light in the synthesis process has been investigated and is discussed in detail. {yields} The antibacterial effect of the synthesized silver nanoparticles has been assessed against both Gram classes of bacteria. {yields} Synthesized silver colloidal solutions were found to be stable for a very long period and retained their bactericidal potential. - Abstract: A green synthetic route for the production of highly stable silver nanoparticles using aqueous garlic extract is being reported for the first time. The silver nanoparticles were synthesized by exposing a mixture of 0.1 M [Ag(NH{sub 3}){sub 2}]{sup +} and diluted aqueous garlic extract under bright sunlight for 15 min. The garlic extract components served as both reducing and capping agents in the synthesis of silver nanoparticles while the sunlight acted as catalyst in the synthesis process. The synthesized nanoparticles were characterized using UV-visible (UV-vis) spectrophotometer; transmission electron microscopy (TEM), glancing angle X-ray diffraction (GA-XRD) and Fourier transform infra red (FTIR) spectrometry. The nanoparticles were found to be poly-dispersed in nature, spherical in shape and of 7.3 {+-} 4.4 nm in size. The FTIR analysis was suggestive of proteins as capping agents around the nanoparticles. The yield of synthesized nanoparticles was calculated to be approximately 80% by dry weight and 85% ICP-AES method. The synthesized silver nanoparticles exhibited good antibacterial potential against both Gram positive and Gram negative bacterial strains, as measured using well diffusion assay. Most importantly, the silver colloidal solutions thus synthesized were found to be stable for

  20. Synthesis and characterization of polypyrrole grafted chitin

    Science.gov (United States)

    Ramaprasad, A. T.; Latha, D.; Rao, Vijayalakshmi

    2017-05-01

    Synthesis and characterization of chitin grafted with polypyrrole (PPy) is reported in this paper. Chitin is soaked in pyrrole solution of various concentrations for different time intervals and polymerized using ammonium peroxy disulphate (APS) as an initiator. Grafting percentage of polypyrrole onto chitin is calculated from weight of chitin before and after grafting. Grafting of polymer is further verified by dissolution studies. The grafted polymer samples are characterized by FTIR, UV-Vis absorption spectrum, XRD, DSC, TGA, AFM, SEM and conductivity studies.

  1. Biological synthesis and characterization of intracellular gold ...

    Indian Academy of Sciences (India)

    Nanoparticles are produced by various methods like physical, chemical, mechanical and biological. Biological me- thods of ... Stable nanoparticles were produced when an aqueous solution of chloroauric acid (HAuCl4) was reduced ... for the biomimetic synthesis and characterization of protein capped silver nanoparticles.

  2. Synthesis and optical characterization of copper nanoparticles ...

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 40; Issue 1. Synthesis and optical characterization of copper nanoparticles prepared by laser ablation. SAMIRA MONIRI MAHMOOD GHORANNEVISS MOHAMMAD REZA HANTEHZADEH MOHSEN ASADI ASADABAD. Volume 40 Issue 1 February 2017 pp 37-43 ...

  3. Synthesis and characterization of gold nanoparticles incorporated ...

    Indian Academy of Sciences (India)

    Synthesis and characterization of gold nanoparticles incorporated bentonite clay for electrocatalytic sensing of arsenic(III). Pankaj Kumar Rastogi Dharmendra Kumar Yadav Shruti Pandey Vellaichamy Ganesan Piyush Kumar Sonkar Rupali Gupta. Regular Articles Volume 128 Issue 3 March 2016 pp 349-356 ...

  4. Synthesis, characterization, antimicrobial activity and molecular ...

    African Journals Online (AJOL)

    Synthesis, characterization, antimicrobial activity and molecular docking studies of combined pyrazol-barbituric acid pharmacophores. Assem Barakat, Bandar M. Al-Qahtani, Abdullah M. Al-Majid, M. Ali Mohammed Rafi Shaik, Mohamed H.M. Al-Agamy, Abdul Wadood ...

  5. Synthesis and characterization of a reduced heteropoly ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 114; Issue 2. Synthesis and characterization of a ... Shivaiah Samar K Das. Inorganic and Analytical Volume 114 Issue 2 April 2002 pp 107-114 ... Compound (1) crystallizes in a cubic space group 3 ¯ , with = 22.2001(6) Å and = 8. The anion [VVO4W 10 VI V 2 ...

  6. Synthesis, characterization and antimicrobial activity of mixed ...

    African Journals Online (AJOL)

    Synthesis, characterization and antimicrobial activity of mixed ascorbic acid - nicotinamide metal complexes. ... The result of the antimicrobial studies showed that the mixed complexes have higher inhibitory activity than the original ligands against the tested bacteria and fungi species. KEY WORDS: Ascorbic acid, ...

  7. Synthesis, spectroscopic characterization and catalytic oxidation ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 123; Issue 3. Synthesis, spectroscopic characterization and catalytic oxidation properties of ONO/ONS donor Schiff base ruthenium(III) complexes containing PPh3/AsPh3. Priyarega M Muthu Tamizh R Karvembu R Prabhakaran K Natarajan. Volume 123 Issue 3 May ...

  8. Synthesis, characterization and photochemistry of a new ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 127; Issue 9. Synthesis, characterization and photochemistry of a new heptamolybdate supported magnesium-aqua coordination complex. Savita S Khandolkar Pallepogu Raghavaiah Bikshandarkoil R Srinivasan. Volume 127 Issue 9 September 2015 pp 1581-1588 ...

  9. HYDROTHERMAL SYNTHESIS AND CHARACTERIZATION OF A ...

    African Journals Online (AJOL)

    Preferred Customer

    HYDROTHERMAL SYNTHESIS AND CHARACTERIZATION OF A BINUCLEAR. COMPLEX AND A COORDINATION POLYMER OF COPPER(II). Masoumeh Tabatabaee1*, Reza Mohamadinasab1, Kazem Ghaini1 and Hamid Reza Khavasi2. 1Department of Chemistry, Islamic Azad University, Yazd Branch, Yazd, Iran.

  10. Synthesis, characterizations and applications of some nanomaterials

    Indian Academy of Sciences (India)

    Home; Journals; Pramana – Journal of Physics; Volume 65; Issue 4. Synthesis, characterizations and applications of some nanomaterials (TiO2 and SiC nanostructured films, organized CNT structures, ZnO structures and CNT{blood platelet clusters). O N Srivastava A Srivastava D Dash D P Singh R M Yadava P R Mishra J ...

  11. Synthesis, characterization and photoluminescence properties of ...

    Indian Academy of Sciences (India)

    Synthesis of a new nano hybrid of 5,10,15,20-mesotetra(4-aminophenyl) porphyrin (TAP) functionalized with multi-walled carbon nanotubes (MWCNTs) through an amide linkage is reported for the first time. ThisMWCNT-TAP hybrid was characterized by Raman, Fourier transform infrared (FT-IR), Transmissionelectron ...

  12. Synthesis, characterization and electrochemical performance of ...

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science; Volume 40; Issue 6. Synthesis, characterization and electrochemical performance of Li 2 Ni x Fe 1 − x SiO 4 cathode materials for lithium ion batteries. A Y SHENOUDA M M S SANAD. Volume 40 Issue 6 October 2017 pp 1055-1060 ...

  13. Synthesis, characterizations and applications of some ...

    Indian Academy of Sciences (India)

    Home; Journals; Pramana – Journal of Physics; Volume 65; Issue 4. Synthesis, characterizations and applications of some nanomaterials (TiO2 and SiC nanostructured films, organized CNT structures, ZnO structures and CNT{blood platelet clusters). O N Srivastava A Srivastava D Dash D P Singh R M Yadava P R Mishra J ...

  14. Zeolite from fly ash: synthesis and characterization

    Indian Academy of Sciences (India)

    Unknown

    disposal or to minimize the environmental impact. One of the approaches is the conversion of fly ash to zeolites, which have wide applications in ion exchange, as mole- cular sieves, catalysts, and adsorbents (Breck 1974). The present study is concerned with the synthesis of zeolite from coal fly ash and its characterization ...

  15. synthesis, characterization, thermal behavior and antimicrobial

    African Journals Online (AJOL)

    The present work deals with the synthesis and characterization of Co, Ni, Cd, Zn and Cu(II) complexes of 3-methyl benzoic acid with/without hydrazine. EXPERIMENTAL. The chemicals and solvents used were of AR grade received from Fluka Chemicals. The double distilled water was used for the preparation and chemical ...

  16. Synthesis and characterization of a reduced heteropoly ...

    Indian Academy of Sciences (India)

    Pope and Flynn reported a series of such compounds of the general formula VtVnW12−n. −+. )3(. 40. O n. (where Vt is the vanadium in the center of the tertrahedron, n = 2, 3, 4)5,6. The synthesis and characterization of these heteropolytungstovanadates have been described many years ago, but the reduced analogue of ...

  17. Studies on Synthesis of Some New Chalcone and Pyrimidines and their Antibacterial Activity

    Directory of Open Access Journals (Sweden)

    Ketan Mistry

    2005-01-01

    Full Text Available Pyrimidine-2-one derivatives, 2-[2-{1ʹ-(p-nitrophenyl-6ʹ-(substituted-phenyl-pyrimidine-2ʹ-one-4ʹ-yl}-hydrazinomethyl]-3-(p-methoxy phenyl-quina-zoline-4(3H-one [4a-j] have been synthesised by the condensation of p-nitro phenylurea and various chalcones, 2-(substituted phenylchalconylhydrazinomethyl-3-(p-methoxyphenyl-quinazoline-4(3H-one [3a-j] in the presence of catalytic amount of conc. HCl. And this series of chalcones have been synthesised by the reaction of 2-acetylhydrazinomethyl-3-(substitutedphenyl-quinazoline-4(3H-one [2] with different substituted aldehydes in presence of a 2% NaOH solution. These products have been characterized by means of elemental analysis, IR and NMR spectrul studies and tested for antibacterial activities against Staphylococcus aureus, Bacillus subtillis, Escherichia coli and Salmonella typhi.

  18. Synthesis, Anticancer and Antibacterial Activity of Salinomycin N-Benzyl Amides

    Directory of Open Access Journals (Sweden)

    Michał Antoszczak

    2014-11-01

    Full Text Available A series of 12 novel monosubstituted N-benzyl amides of salinomycin (SAL was synthesized for the first time and characterized by NMR and FT-IR spectroscopic methods. Molecular structures of three salinomycin derivatives in the solid state were determined using single crystal X-ray method. All compounds obtained were screened for their antiproliferative activity against various human cancer cell lines as well as against the most problematic bacteria strains such as methicillin-resistant Staphylococcus aureus (MRSA and Staphylococcus epidermidis (MRSE, and Mycobacterium tuberculosis. Novel salinomycin derivatives exhibited potent anticancer activity against drug-resistant cell lines. Additionally, two N-benzyl amides of salinomycin revealed interesting antibacterial activity. The most active were N-benzyl amides of SAL substituted at -ortho position and the least anticancer active derivatives were those substituted at the -para position.

  19. Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract

    Directory of Open Access Journals (Sweden)

    Upendra Nagaich

    2016-01-01

    Full Text Available The advancement of the biological production of nanoparticles using herbal extracts performs a significant role in nanotechnology discipline as it is green and does not engage harsh chemicals. The objective of the present investigation was to extract flavonoids in the mode of apple extract and synthesize its silver nanoparticles and ultimately nanoparticles loading into hydrogels. The presence of flavonoids in apple extract was characterized by preliminary testing like dil. ammonia test and confirmatory test by magnesium ribbon test. The synthesized silver nanoparticles were characterized using UV spectroscopy, particle size and surface morphology, and zeta potential. Silver nanoparticles loaded hydrogels were evaluated for physical appearance, pH, viscosity, spreadability, porosity, in vitro release, ex vivo permeation, and antibacterial (E. coli and S. aureus and antioxidant studies (DPPH radical scavenging assay. Well dispersed silver nanoparticles below were observed in scanning electron microscope image. Hydrogels displayed in vitro release of 98.01%  ±  0.37% up to 24 h and ex vivo permeation of 98.81  ±  0.24% up to 24 h. Hydrogel effectively inhibited the growth of both microorganism indicating good antibacterial properties. The value of percent radical inhibition was 75.16%  ±  0.04 revealing its high antioxidant properties. As an outcome, it can be concluded that antioxidant and antiageing traits of flavonoids in apple extract plus biocidal feature of silver nanoparticles can be synergistically and successfully utilized in the form of hydrogel.

  20. Amine-functionalized, silver-exchanged zeolite NaY: Preparation, characterization and antibacterial activity

    Science.gov (United States)

    Hanim, Siti Aishah Mohd; Malek, Nik Ahmad Nizam Nik; Ibrahim, Zaharah

    2016-01-01

    Amine-functionalized, silver-exchanged zeolite NaY (ZSA) were prepared with three different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.20 and 0.40 M) and four different concentrations of silver ions (25%, 50%, 100% and 200% from zeolite cation exchange capacity (CEC)). The samples were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX), surface area analysis, thermogravimetric analysis (TGA) and zeta potential (ZP) analysis. The FTIR results indicated that the zeolite was functionalized by APTES and that the intensity of the peaks corresponding to APTES increased as the concentration of APTES used was increased. The antibacterial activities of the silver-exchanged zeolite NaY (ZS) and ZSA were studied against Escherichia coli ATCC11229 and Staphylococcus aureus ATCC6538 using the disc diffusion technique (DDT) and minimum inhibitory concentration (MIC). The antibacterial activity of ZSA increased with the increase in APTES on ZS, and E. coli was more susceptible towards the sample compared to S. aureus. The FESEM micrographs of the bacteria after contact with the ZSA suggested different mechanisms of bacterial death for these two bacteria due to exposure to the studied sample. The functionalization of ZS with APTES improved the antibacterial activity of the silver-zeolite, depending on the concentration of silver ions and APTES used during modification.

  1. Preparation, characterization and antibacterial applications of carboxymethyl chitosan/CuO nanocomposite hydrogels.

    Science.gov (United States)

    Wahid, Fazli; Wang, Hai-Song; Lu, Yu-Shi; Zhong, Cheng; Chu, Li-Qiang

    2017-08-01

    In this study, a series of antibacterial carboxymethyl chitosan/CuO nanocomposites were prepared by treating the carboxymethyl chitosan (CMCh) hydrogel with copper (II) chloride (CuCl 2 ) solution followed by the oxidation of copper ions in the presence of sodium hydroxide. The resulting nanocomposite hydrogels were characterized by using FTIR, XRD and SEM techniques. The SEM micrographs revealed the uniform distribution of CuO nanoparticles in the hydrogel matrix with the size ranging from 20nm to 50nm, while XRD patterns confirmed the formation of monoclinic crystalline structures of CuO nanoparticles. The swelling behavior of the nanocomposite hydrogels were studied at a varied pH range. CMCh/CuO nanocomposite hydrogels showed rather higher swelling as compared to pure CMCh hydrogel. The antibacterial properties of the nanocomposite hydrogels were studied against Staphylococcus aureus and Escherichia coli. The results showed excellent antibacterial behavior of the nanocomposite hydrogels. Therefore, the developed CMCh/CuO nanocomposite hydrogels might be used effectively in numerous biomedical fields. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract.

    Science.gov (United States)

    Alavi, Mehran; Karimi, Naser

    2017-12-12

    Recently, major problem related to pathogenic bacteria is augmentation of antibiotic resistance which has been changed treatment and recovery of millions of infectious patients. The present study reports an eco-friendly, rapid and easy method for synthesis of silver (Ag), copper (Cu) and titanium dioxide (TiO 2 ) nanoparticles (NPs) using Artemisia haussknechtii leaf aqueous extract with antibacterial activities against multi-drug resistance (MDR) bacteria species. Three different concentrations (0.001, 0.01 and 0.1 M) of AgNO 3 , CuSO 4 and TiO (OH) 2 were investigated for obtaining optimum NPs green synthesis. Total phenolic content, total flavonoid content of leaf extract and total antioxidant activity (DPPH) assay were determined as radical scavenging methods. UV-Visible spectroscopy, Fourier transform infrared spectroscopy analysis, X-ray diffraction, energy dispersive X-ray spectroscopy, field emission scanning electron microscope and atomic force microscopy (AFM) were used due to NPs characterization. The size average of the Ag, Cu and TiO 2 NPs obtained were respectively 10.69 ± 5.55, 35.36 ± 44.4 and 92.58 ± 56.98 nm. In the case of antibacterial assay, disc diffusion assay, minimum inhibitory concentration, minimum bactericidal concentration, bacterial growth and morphology of four MDR species Staphylococcus aureus ATCC 43300, Staphylococcus epidermidis ATCC 12258, Serratia marcescens ATTC13880 and Escherichia coli ATCC 25922 were evaluated. Results of this study demonstrated that A. haussknechtii leaf extract with various groups of phytochemicals such as phenols and flavonoids had suitable ability in green synthesis of Ag, Cu and TiO 2 NPs. Also, Ag and Cu NPs had more antibacterial activities compared to TiO 2 NPs.

  3. Green synthesis of Ag nanoparticles using Tamarind fruit extract for the antibacterial studies.

    Science.gov (United States)

    Jayaprakash, N; Vijaya, J Judith; Kaviyarasu, K; Kombaiah, K; Kennedy, L John; Ramalingam, R Jothi; Munusamy, Murugan A; Al-Lohedan, Hamad A

    2017-04-01

    In the present study, first time we report the microwave-assisted green synthesis of silver nanoparticles (AgNPs) using Tamarindus indica natural fruit extract. The plant extract plays a dual role of reducing and capping agent for the synthesis of AgNPs. The formation of spherical shape AgNPs is confirmed by XRD, HR-SEM, and HR-TEM. The presence of face-centered cubic (FCC) silver is confirmed by XRD studies and the average crystallite size of AgNPs is calculated to be around 6-8nm. The average particle diameter is found to be around 10nm, which is identified from HR-TEM images. The purity of AgNPs is confirmed by EDX analysis. The presence of sigmoid curve in UV-Visible absorption spectra suggests that the reaction has complicated kinetic features. To investigate the functional groups of the extract and their involvement in the reduction of AgNO 3 to form AgNPs, FT-IR studies are carried out. The redox peaks are observed in cyclic voltammetry in the potential range of -1.2 to +1.2V, due to the redox active components of the T. indica fruit extract. In photoluminescence spectroscopy, the excited and emission peaks were obtained at 432nm and 487nm, respectively. The as-prepared AgNPs showed good results towards antibacterial activities. Hence, the present approach is a facile, cost- effective, reproducible, eco-friendly, and green method. Copyright © 2017. Published by Elsevier B.V.

  4. Synthesis, characterization, photoluminescence and thermally ...

    Indian Academy of Sciences (India)

    Administrator

    Abstract. Sm3+-doped ZnAl2O4 phosphor was synthesized by citrate sol–gel method and characterized using. X-ray diffraction and scanning electron microscopy to identify the crystalline phase and determine the parti- cle size. Photoluminescence (PL) studies on the sample showed emission peaks at 563, 601, 646 and ...

  5. SYNTHESIS AND CHARACTERIZATION OF NANOCOMPOSITE ...

    African Journals Online (AJOL)

    ABSTRACT. In this work, nano ferrite spinel NiFe2O4 was synthesized by sol-gel method and characterized by. SEM, XRD, FT-IR, and VSM. In second step Schiff base made from salicylaldehyde and amino propyl triethoxy silane was used for modification of the synthesized nano ferrit. In the third step removal of Ni(II) was ...

  6. Synthesis, characterization, photoluminescence and thermally ...

    Indian Academy of Sciences (India)

    Administrator

    grinding and heating in (Ar + 10% H2) atmosphere. 2.2 Sample characterization. The as-synthesized ... with literature value of cell parameter a = 8⋅059 A. No impurity phase was observed. The XRD pattern was ..... are thankful to Dr N D Dahale, Fuel Chemistry Division,. BARC, Dr T K Seshagiri, former scientist, and Shri.

  7. One-step synthesis of carbohydrate esters as antibacterial and antifungal agents.

    Science.gov (United States)

    AlFindee, Madher N; Zhang, Qian; Subedi, Yagya Prasad; Shrestha, Jaya P; Kawasaki, Yukie; Grilley, Michelle; Takemoto, Jon Y; Chang, Cheng-Wei Tom

    2018-02-01

    Carbohydrate esters are biodegradable, and the degraded adducts are naturally occurring carbohydrates and fatty acids which are environmentally friendly and non-toxic to human. A simple one-step regioselective acylation of mono-carbohydrates has been developed that leads to the synthesis of a wide range of carbohydrate esters. Screening of these acylated carbohydrates revealed that several compounds were active against a panel of bacteria and fungi, including Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Candida albicans, Cryptococcus neoformans, Aspergillus flavus and Fusarium graminearum. Unlike prior studies on carbohydrate esters that focus only on antibacterial applications, our compounds are found to be active against both bacteria and fungi. Furthermore, the synthetic methodology is suitable to scale-up production for a variety of acylated carbohydrates. The identified lead compound, MAN014, can be used as an antimicrobial in applications such as food processing and preservation and for treatment of bacterial and fungal diseases in animals and plants. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Synthesis of oleic acid-stabilized silver nanoparticles and analysis of their antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Le, Anh-Tuan, E-mail: tuanla-hast@mail.hut.edu.vn [Department of Nanoscience and Nanotechnology, Hanoi Advanced School of Science and Technology (HAST), Hanoi University of Technology, F Building, 40 Ta Quang Buu street, Hanoi (Viet Nam); Tam, Le Thi; Tam, Phuong Dinh; Huy, P.T [Department of Nanoscience and Nanotechnology, Hanoi Advanced School of Science and Technology (HAST), Hanoi University of Technology, F Building, 40 Ta Quang Buu street, Hanoi (Viet Nam); Huy, Tran Quang [National Institute of Hygiene and Epidemiology (NIHE), 01 Yersin, Hai Ba Trung District, Hanoi (Viet Nam); Van Hieu, Nguyen [International Training Institute of Materials Science (ITIMS), Hanoi University of Technology, No 1 Dai Co Viet, Hanoi (Viet Nam); Kudrinskiy, A A; Krutyakov, Yu A [Department of Chemistry, M V Lomonosov Moscow State University, Leninskie Gory, 119991 Moscow (Russian Federation)

    2010-07-20

    The development of new and simple green chemical methods for synthesizing colloidal solutions of functional nanoparticles is desirable for environment-friendly applications. In the present work, we report a feasible method for synthesizing colloidal solutions of silver nanoparticles (Ag NPs) based on the modified Tollens technique. The Ag NPs were stabilized by using oleic acid as a surfactant and were produced for the first time by the reduction of silver ammonium complex [Ag(NH{sub 3}){sub 2}]{sup +}{sub (aq)} by glucose with UV irradiation treatment. A stable and nearly monodisperse aqueous Ag NPs solution with average-sized particles ({approx} 9-10 nm) was obtained. The Ag NPs exhibited high antibacterial activity against both Gram-negative Escherichia Coli (E. coli) and Gram-positive Staphylococcus aureus bacteria. Electron microscopic images and analyses provided further insights into the interaction and bactericidal mechanism of the Ag NPs. The proposed method of synthesis is an effective way to produce highly bactericidal colloidal solutions for medical, microbiological, and industrial applications.

  9. Preparation, characterization, and antibacterial activity studies of silver-loaded poly(styrene-co-acrylic acid) nanocomposites

    International Nuclear Information System (INIS)

    Song, Cunfeng; Chang, Ying; Cheng, Ling; Xu, Yiting; Chen, Xiaoling; Zhang, Long; Zhong, Lina; Dai, Lizong

    2014-01-01

    A simple method for preparing a new type of stable antibacterial agent was presented. Monodisperse poly(styrene-co-acrylic acid) (PSA) nanospheres, serving as matrices, were synthesized via soap-free emulsion polymerization. Field-emission scanning electron microscopy micrographs indicated that PSA nanospheres have interesting surface microstructures and well-controlled particle size distributions. Silver-loaded poly(styrene-co-acrylic acid) (PSA/Ag-NPs) nanocomposites were prepared in situ through interfacial reduction of silver nitrate with sodium borohydride, and further characterized by transmission electron microscopy and X-ray diffraction. Their effects on antibacterial activity including inhibition zone, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bactericidal kinetics were evaluated. In the tests, PSA/Ag-NPs nanocomposites showed excellent antibacterial activity against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli. These nanocomposites are considered to have potential application in antibacterial coatings on biomedical devices to reduce nosocomial infection rates. - Highlights: • A new type of antibacterial agent (PSA/Ag-NPs nanocomposites) was synthesized. • The antibacterial activity against S. aureus and E. coli was studied. • Inhibition zone, MIC, MBC, and bactericidal kinetics were evaluated. • PSA/Ag-NPs nanocomposites showed excellent antibacterial activity

  10. Preparation, characterization, and antibacterial activity studies of silver-loaded poly(styrene-co-acrylic acid) nanocomposites

    Energy Technology Data Exchange (ETDEWEB)

    Song, Cunfeng [Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005 (China); Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China); Chang, Ying; Cheng, Ling; Xu, Yiting [Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005 (China); Chen, Xiaoling, E-mail: tinachen0628@163.com [Department of Endodontics, Xiamen Stomatology Hospital, Teaching Hospital of Fujian Medical University, Xiamen 361003 (China); Zhang, Long; Zhong, Lina [Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005 (China); Dai, Lizong, E-mail: lzdai@xmu.edu.cn [Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005 (China)

    2014-03-01

    A simple method for preparing a new type of stable antibacterial agent was presented. Monodisperse poly(styrene-co-acrylic acid) (PSA) nanospheres, serving as matrices, were synthesized via soap-free emulsion polymerization. Field-emission scanning electron microscopy micrographs indicated that PSA nanospheres have interesting surface microstructures and well-controlled particle size distributions. Silver-loaded poly(styrene-co-acrylic acid) (PSA/Ag-NPs) nanocomposites were prepared in situ through interfacial reduction of silver nitrate with sodium borohydride, and further characterized by transmission electron microscopy and X-ray diffraction. Their effects on antibacterial activity including inhibition zone, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bactericidal kinetics were evaluated. In the tests, PSA/Ag-NPs nanocomposites showed excellent antibacterial activity against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli. These nanocomposites are considered to have potential application in antibacterial coatings on biomedical devices to reduce nosocomial infection rates. - Highlights: • A new type of antibacterial agent (PSA/Ag-NPs nanocomposites) was synthesized. • The antibacterial activity against S. aureus and E. coli was studied. • Inhibition zone, MIC, MBC, and bactericidal kinetics were evaluated. • PSA/Ag-NPs nanocomposites showed excellent antibacterial activity.

  11. Physicochemical characterization and antibacterial activity ofRajata Bhasmaand silver nanoparticle.

    Science.gov (United States)

    Sharma, Revati; Bhatt, Ahsan; Thakur, Mansee

    2016-01-01

    Bhasma , an Ayurvedic metallo-mineral preparation, is claimed to be biologically produced nanoparticles. Rajata (silver) is a noble metal known for its antimicrobial activity. Rajata Bhasma (RB) is expected to be composed of nanoparticles. With all these facts in place, this study was conducted to evaluate RB for the presence of silver nanoparticle (SNP) and its antimicrobial effect. The aim of this study is to analyze the physicochemical characterization, antibacterial activity of RB, and SNP. RB was commercially ordered and SNP was prepared by Turkevich method. Characterization of RB was carried out by scanning electron microscopy (SEM) and inductively coupled plasma-atomic emission spectroscopy (ICP-AES). SNP was characterized by ultraviolet (UV)-visible spectroscopy, transmission electron microscopy (TEM), and ICP-AES. Antibacterial activity of RB and SNP was carried out by well-diffusion method. Analysis of RB by SEM revealed particles in range from 10 to 60 nm. UV-visible spectrum of the aqueous medium containing SNPs showed absorption peak at around 423 nm. The TEM analysis showed that SNP was spherical in the range of 5-50 nm and uniformly distributed without significant agglomeration. The content of silver in RB measured with ICP-AES was found to be 70.56% whereas in case of SNP was 65.23%. Staphylococcus aureus was found to be sensitive to both RB and SNP. Escherichia coli , Pseudomonas aeruginosa , and Enterococcus faecalis were found to be resistant to RB as well as SNP. The current study shows that RB does have silver particles in the size of nanometers and also has mild antibacterial activity.

  12. Physicochemical and antibacterial characterization of ionocity Ag/Cu powder nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Nowak, A., E-mail: ana.maria.nowak@gmail.com [A. Chełkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice (Poland); Silesian Center for Education and Interdisciplinary Research, 75 Pułku Piechoty 1A, 41-500 Chorzów (Poland); Szade, J. [A. Chełkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice (Poland); Silesian Center for Education and Interdisciplinary Research, 75 Pułku Piechoty 1A, 41-500 Chorzów (Poland); Talik, E. [A. Chełkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice (Poland); Zubko, M. [Silesian Center for Education and Interdisciplinary Research, 75 Pułku Piechoty 1A, 41-500 Chorzów (Poland); Institute of Material Science, University of Silesia, 75 Pułku Piechoty 1a, 41-500 Chórzow (Poland); Wasilkowski, D. [Department of Biochemistry, University of Silesia, Jagiellońska 28, 40-032 Katowice (Poland); Dulski, M. [Silesian Center for Education and Interdisciplinary Research, 75 Pułku Piechoty 1A, 41-500 Chorzów (Poland); Institute of Material Science, University of Silesia, 75 Pułku Piechoty 1a, 41-500 Chórzow (Poland); Balin, K. [A. Chełkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice (Poland); Silesian Center for Education and Interdisciplinary Research, 75 Pułku Piechoty 1A, 41-500 Chorzów (Poland); and others

    2016-07-15

    Metal ion in bimetallic nanoparticles has shown vast potential in a variety of applications. In this paper we show the results of physical and chemical investigations of powder Ag/Cu nanoparticles obtained by chemical synthesis. Transmission electron microscopy (TEM) experiment indicated the presence of bimetallic nanoparticles in the agglomerated form. The average size of silver and copper nanoparticles is 17.1(4) nm (Ag) and 28.9(2) nm (Cu) basing on the X-ray diffraction (XRD) data. X-ray photoelectron (XPS) and Raman spectroscopies revealed the existence of metallic silver and copper as well as Cu{sub 2}O and CuO being a part of the nanoparticles. Moreover, UV–Vis spectroscopy showed surface alloy of Ag and Cu while Time of Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) and Energy Dispersive X-ray Spectroscopy (EDX) showed heterogeneously distributed Ag structures placed on spherical Cu nanoparticles. The tests of antibacterial activity show promising killing/inhibiting growth behaviour for Gram positive and Gram negative bacteria. - Highlights: • Ag/Cu nanoparticles were obtained in the powder form. • The average size of nanoparticles is 17.1(4) nm (Ag) and 28.9(2) nm (Cu). • Ag/Cu powder nanoparticle shows promising antibacterial properties.

  13. Green biosynthesis of gold nanoparticles using Galaxaura elongata and characterization of their antibacterial activity

    Directory of Open Access Journals (Sweden)

    Neveen Abdel-Raouf

    2017-05-01

    Full Text Available The synthesis of gold nanoparticles (Au using Galaxaura elongata (powder or extract is demonstrated here. The rapid formation of stable Au nanoparticles has been found using G. elongata extract in aqueous medium at normal atmospheric condition. Transmission electron microscopy (TEM analysis revealed that the particles are spherical in shape along with a few rod, triangular, truncated triangular and hexagonal shaped nanoparticles. Zeta potential measurements indicated that the Au nanoparticles were in the size range of 3.85–77.13 nm. Fourier transform infrared spectroscopy (FTIR showed that nanoparticles were capped with alga compounds. The chemical constituents, viz. Andrographolide, Alloaromadendrene oxide, glutamic acid, hexadecanoic acid, oleic acid, 11-eicosenoic acid, stearic acid, gallic acid, Epigallocatechin Catechin and Epicatechin gallate of the algal extract were identified which may act as a reducing, stabilizing and capping agent. The nanoparticles were also evaluated for their antibacterial activities which showed better antibacterial effects with maximum inhibition zones of 17–16 mm by AuNPs synthesized by ethanolic extract against Escherichia coli, Klebsiella pneumoniae and MRSA, respectively, followed by Staphylococcus aureus and Pseudomonas aeruginosa (13 mm. Furthermore, the nanoparticles synthesized by the powder of G. elongata were found to be highly effective against E. coli and K. pneumoniae (13.5 and 13 mm, respectively. On the other hand, the free ethanolic extract of G. elongata exhibits high activity only against MRSA (14 mm.

  14. Extraction and Characterization of Natural Dye from Green Walnut Shells and Its Use in Dyeing Polyamide: Focus on Antibacterial Properties

    Directory of Open Access Journals (Sweden)

    Mohammad Mirjalili

    2013-01-01

    Full Text Available Extraction of dyes from walnut using Soxhlet apparatus has been studied. The color components extracted and isolated from walnut shells were characterized by column chromatography, thin layer chromatography (TLC, nuclear magnetic resonance (NMR, mass spectroscopy (MS, and infrared (IR techniques. Natural dye extract obtained from the walnut was used in dyeing polyamide fabrics with different mordants. The dyed fabrics were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus and Gram-negative (Escherichia coli bacteria. As such, the relationship between antibacterial activity and dye concentration is investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with walnut displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate and exhibited good and durable fastness properties.

  15. Synthesis, characterization and biological evaluation of novel diesters of 4,4'-dihydroxy azoxy benzene with long chain carboxylic acid

    International Nuclear Information System (INIS)

    Shehzadi, S.; Siddiqi, H.M.; Qasim, M.M.

    2014-01-01

    Synthesis of novel symmetrical azoxy diesters have been prepared by the reaction of 4,4'-dihydroxyazoxy benzene with aliphatic acid halides of varying chain lengths. The synthesized compounds have been characterized by spectral and analytical means. These symmetrical azoxy diesters exhibit good antifungal against six fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Alternaria solani, Fusarium solani and Aspergillus fumigatus) and antitumor activities while no significant antibacterial activity has been observed. These synthesized compounds are also potent free radical scavengers. (author)

  16. Manganese(II chelates of bioinorganic and medicinal relevance: Synthesis, characterization, antibacterial activity and 3D-molecular modeling of some penta-coordinated manganese(II chelates in O,N-donor coordination matrix of β-diketoenolates and picolinate

    Directory of Open Access Journals (Sweden)

    R.C. Maurya

    2016-09-01

    Full Text Available Four new mixed-ligand complexes of manganese(II of the composition [Mn(pa(L(H2O], where paH = picolinic acid and LH = acetoacetanilide (aaH, o-acetoacetanisidide (o-aansH, o-acetoacetotoluidide (o-aatdH or ethylacetoacetate (eacacH, have been synthesized by the interaction of MnCl2·4H2O with the said ligands in aqueous-ethanol medium. The complexes so obtained have been characterized on the basis of elemental analyses, molar conductance and magnetic measurements, thermogravimetric analyses, mass, EPR, infrared and electronic spectral studies. Synthesized complexes have shown significantly greater antibacterial activity against Escherichia coli and Vibrio cholera than ligands. The 3D-molecular modeling and analysis for bond lengths and bond angles have also been carried out for one of the representative compound [Mn(pa(aa(H2O] (1 to substantiate the proposed structures.

  17. Photocatalytic semiconductors synthesis, characterization, and environmental applications

    CERN Document Server

    Hernández-Ramírez, Aracely

    2014-01-01

    This critical volume examines the different methods used for the synthesis of a great number of photocatalysts, including TiO2, ZnO and other modified semiconductors, as well as characterization techniques used for determining the optical, structural and morphological properties of the semiconducting materials. Additionally, the authors discuss photoelectrochemical methods for determining the light activity of the photocatalytic semiconductors by means of measurement of properties such as band gap energy, flat band potential and kinetics of hole and electron transfer. Photocatalytic Semiconductors: Synthesis, Characterization and Environmental Applications provide an overview of the semiconductor materials from first- to third-generation photocatalysts and their applications in wastewater treatment and water disinfection. The book further presents economic and toxicological aspects in the production and application of photocatalytic materials.

  18. Eco-friendly synthesis of silver nanoparticles using green algae (Caulerpa serrulata): reaction optimization, catalytic and antibacterial activities.

    Science.gov (United States)

    Aboelfetoh, Eman F; El-Shenody, Rania A; Ghobara, Mohamed M

    2017-07-01

    Stable colloidal silver nanoparticles (AgNPs) were synthesized using Caulerpa serrulata (green marine algae) aqueous extract as an efficient reducing and stabilizing agent. This method is considered to be a sustainable alternate to the more complicated chemical procedures. To achieve the optimization synthesis of AgNPs, several effects such as extract concentration, contact time, pH values, and temperature were examined. The synthesized AgNPs were characterized by UV-Vis spectroscopy, FT-IR, XRD, and HR-TEM. The synthesized AgNPs showed an intense surface plasmon resonance band at 412 nm at the optimal conditions (20% (v/v) extract and 95 °C). TEM reveal that higher extract concentration and higher temperature leading to the formation of spherical AgNPs with an average particle size of 10 ± 2 nm. The synthesized AgNPs showed excellent catalytic reduction activity of Congo red (CR) dye from aqueous solutions. The degradation percentage of CR with AgNPs accelerated by increasing either NaBH 4 concentration or catalytic dosage. The AgNPs synthesized at higher temperature (e.g., 10Ag-95) exhibited the highest catalytic activity. The reaction kinetics was found to be pseudo first order with respect to the dye concentration. Moreover, the AgNPs displayed antibacterial activity at lower concentration against Staphylococcus aureus, Pseudomonas aeruginosa, Shigella sp., Salmonella typhi, and Escherichia coli and may be a good alternative therapeutic approach. The outcomes of the current study confirmed that the synthesized AgNPs had an awesome guarantee for application in catalysis and wastewater treatment.

  19. Polypyrrole-silver Nanocomposite: Synthesis and Characterization

    OpenAIRE

    D. M. Nerkar; S. V. Panse; S. P. Patil; S. E. Jaware; G. G. Padhye

    2016-01-01

    Polypyrrole-Silver (PPy-Ag) nanocomposite has been successfully synthesized by the chemical oxidative polymerization of pyrrole with iron (III) chloride as an oxidant, in the presence of a colloidal suspension of silver nanoparticles. Turkevich method (Citrate reduction method) was used for the synthesis of silver nanoparticles (Ag NPs). The silver nanoparticles were characterized by UV-Visible spectroscopy which showed an absorption band at 423 nm confirming the formation of nanoparticles. P...

  20. Synthesis, structural and electrical characterizations of thermally ...

    African Journals Online (AJOL)

    Synthesis, structural and electrical characterizations of thermally evaporated Cu 2 SnS 3 thin films. ... The surface profilometer shows that the deposited films are rough. The XRD spectra identified the ... The electrical resistivity of the deposited Cu2SnS3 film is 2.55 x 10-3 Ωcm. The conductivity is in the order of 103 Ω-1cm-1.

  1. Synthesis and characterization of -phosphorylated thioureas ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 122; Issue 3. Synthesis and characterization of -phosphorylated thioureas RNHC(S)NHP(O)(OPr)2 (R = 2-MeC6H4, 2,6-Me2C6H3, 2,4,6-Me3C6H2). Damir A Safin Maria G Babashkina Michael Bolte Axel Klein. Full Papers Volume 122 Issue 3 May 2010 pp 409- ...

  2. Synthesis and characterization of a reduced heteropoly ...

    Indian Academy of Sciences (India)

    compounds of the general formula VtVnW12−n. −+. )3(. 40. O n. (where Vt is the vanadium in the center of the tertrahedron, n = 2, 3, 4)5,6. The synthesis and characterization of these heteropolytungstovanadates have been described many years ago, but the reduced analogue of any of these in the series has yet to be ...

  3. Green Synthesis of Silver Nanoparticles Using Leaf Extracts of Clitoria ternatea and Solanum nigrum and Study of Its Antibacterial Effect against Common Nosocomial Pathogens

    International Nuclear Information System (INIS)

    Krithiga, N.; Rajalakshmi, A.; Jayachitra, A.

    2015-01-01

    Bionanotechnology has emerged up as integration between biotechnology and nano technology for developing biosynthetic and environmental friendly technology for synthesis of nano materials. Silver has been known to have effective bactericidal properties for centuries. Nowadays, silver based topical dressings have been widely used as a treatment for infection in burns, open wounds, and chronic ulcer. As the pathogenic organisms are getting evolved day by day due to mutation and gaining antibiotic resistance, an important industrial sector of nano science deals with the preparation and study of nanoparticles in antibacterial clothing, burn ointments, and coating for medical device. The size of nano materials is much smaller than that of most biological molecules and structures; therefore, nano materials can be useful in both in vivo and in vitro biomedical research application. The purpose of the study is to synthesize and characterize the plant mediated silver nanoparticles using Clitoria ternatea and Solanum nigrum. Further investigation of the shape and size of nanoparticle was done by X-ray diffraction and scanning electron microscopic studies. A silver nanoparticle at different concentration was assessed for its antibacterial effect, against various nosocomial pathogens.

  4. Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles.

    Science.gov (United States)

    Anbazhagan, Sathiyaseelan; Azeez, Shajahan; Morukattu, Girilal; Rajan, Ramachandran; Venkatesan, Kaviyarasan; Thangavelu, Kalaichelvan Puthupalayam

    2017-10-01

    Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV-Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs showed the maximum absorbance at 420-430 nm. The transmission electron micrograph revealed the formation of considerably uniform-sized AgNPs with an average size of 20-50 nm. The reducing and capping agents responsible for AgNP synthesis were identified by FT-IR. AgNP-incorporated cotton fabrics exhibited promising antibacterial activity against pathogenic bacteria. In addition, the in vitro cell viability of Vero cells (African green monkey kidney cells) was analyzed and the IC 50 value of AgNPs was found to be 62.8 µg/mL. Taken together, these results clearly reveal less toxic AgNPs which could be exploited for various biomedical applications.

  5. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches

    Directory of Open Access Journals (Sweden)

    Xi-Feng Zhang

    2016-09-01

    Full Text Available Recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Silver nanoparticles (AgNPs are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. AgNPs play an important role in nanoscience and nanotechnology, particularly in nanomedicine. Although several noble metals have been used for various purposes, AgNPs have been focused on potential applications in cancer diagnosis and therapy. In this review, we discuss the synthesis of AgNPs using physical, chemical, and biological methods. We also discuss the properties of AgNPs and methods for their characterization. More importantly, we extensively discuss the multifunctional bio-applications of AgNPs; for example, as antibacterial, antifungal, antiviral, anti-inflammatory, anti-angiogenic, and anti-cancer agents, and the mechanism of the anti-cancer activity of AgNPs. In addition, we discuss therapeutic approaches and challenges for cancer therapy using AgNPs. Finally, we conclude by discussing the future perspective of AgNPs.

  6. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches

    Science.gov (United States)

    Zhang, Xi-Feng; Liu, Zhi-Guo; Shen, Wei; Gurunathan, Sangiliyandi

    2016-01-01

    Recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. AgNPs play an important role in nanoscience and nanotechnology, particularly in nanomedicine. Although several noble metals have been used for various purposes, AgNPs have been focused on potential applications in cancer diagnosis and therapy. In this review, we discuss the synthesis of AgNPs using physical, chemical, and biological methods. We also discuss the properties of AgNPs and methods for their characterization. More importantly, we extensively discuss the multifunctional bio-applications of AgNPs; for example, as antibacterial, antifungal, antiviral, anti-inflammatory, anti-angiogenic, and anti-cancer agents, and the mechanism of the anti-cancer activity of AgNPs. In addition, we discuss therapeutic approaches and challenges for cancer therapy using AgNPs. Finally, we conclude by discussing the future perspective of AgNPs. PMID:27649147

  7. Green synthesis of chalcones derivatives as intermediate of flavones and their antibacterial activities

    Science.gov (United States)

    VH, Elfi Susanti; Matsjeh, Sabirin; Wahyuningsih, Tutik Dwi; Mustofa, Redjeki, Tri

    2016-02-01

    Four chalcones derivatives have been synthesized from 3,4-dimethoxybenzaldehyde and acetophenone derivatives (2-hydroxy acetophenone, 2,4-dihydroxy acetophenone, 2,5-dihydroxy acetophenone and 2,6-dihydroxy acetophenone). The synthesis of these chalcones were conducted by Claisen-Schmidt condensation using grinding techniques at room temperature in the absence of solvents. The chalcones were prepared by grinding together equivalent amount of the approriate hydroxyacetophenone and 3,4-dimethoxybenzaldehyde in the presence of solid sodium hydroxide. Grinding techniques for synthesis of the chalcones derivatives is simple, efficient and environmentally benign compared to conventional methods. Then, the four chalcones derivatives undergo cyclization reactions to produce four flavones after reacted with iodine. The synthesized compounds were characterized by spectrometry (IR, 1H-NMR, 13C-NMR and MS).

  8. Green synthesis of chalcones derivatives as intermediate of flavones and their antibacterial activities

    Energy Technology Data Exchange (ETDEWEB)

    VH, Elfi Susanti, E-mail: elsantivh@yahoo.com; Redjeki, Tri, E-mail: tri-redjeki@yahoo.com [Universitas Sebelas Maret, Ir Sutami 36A Surakarta Indonesia, 57126 (Indonesia); Matsjeh, Sabirin, E-mail: sabirin-mara@yahoo.com; Wahyuningsih, Tutik Dwi, E-mail: mustofajogya@yahoo.co.id [Department of Chemistry FMJPA Universitas Gadjah Mada, Jl Sekip Utara, Yogyakarta Indonesia 55281 (Indonesia); Mustofa, E-mail: tutikdw@hotmail.com [Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta Jl. Sekip Utara Yogyakarta Indonesia, 55281 (Indonesia)

    2016-02-08

    Four chalcones derivatives have been synthesized from 3,4-dimethoxybenzaldehyde and acetophenone derivatives (2-hydroxy acetophenone, 2,4-dihydroxy acetophenone, 2,5-dihydroxy acetophenone and 2,6-dihydroxy acetophenone). The synthesis of these chalcones were conducted by Claisen-Schmidt condensation using grinding techniques at room temperature in the absence of solvents. The chalcones were prepared by grinding together equivalent amount of the approriate hydroxyacetophenone and 3,4-dimethoxybenzaldehyde in the presence of solid sodium hydroxide. Grinding techniques for synthesis of the chalcones derivatives is simple, efficient and environmentally benign compared to conventional methods. Then, the four chalcones derivatives undergo cyclization reactions to produce four flavones after reacted with iodine. The synthesized compounds were characterized by spectrometry (IR, {sup 1}H-NMR, {sup 13}C-NMR and MS)

  9. Synthesis, structural, DFT studies, docking and antibacterial activity of a xanthene based hydrazone ligand

    Science.gov (United States)

    Naseem, Saira; Khalid, Muhammad; Tahir, Muhammad Nawaz; Halim, Mohammad A.; Braga, Ataualpa A. C.; Naseer, Muhammad Moazzam; Shafiq, Zahid

    2017-09-01

    Herein, we present the synthesis of novel xanthene-based hydrazone (1). The chemical structure of 1 was resolved using spectroscopic techniques such as NMR, FT-IR, UV-VIS and X-ray crystallographic approaches. X-ray diffraction analysis shows that the compound (1) crystallizes in triclinic crystal lattice with the Pbar1 space group and diffused to form multi-layered structure due to non-covalent interactions such as intramolecular hydrogen bonding (H.B). In addition to experimental investigation, density functional theory (DFT) calculation with M06-2X/6-31G(d,p) and B3LYP/6-31G(d,p) level of theories was performed on compound (1) to obtain optimized geometry, spectroscopic and electronic properties. DFT optimized geometry shows good agreement with the experimental XRD structure. The hyper conjugative interactions and hydrogen bonding network are responsible for the stability of compound (1) as revealed by natural bond orbital (NBO) calculation. Moreover, hydrogen bonding network in the dimer is confirmed by FT-IR and thermodynamic studies showing excellent agreement with XRD and NBO findings. TD-DFT/UV-VIS analysis provides insight that maximum excitation is found in 1 which shows good agreement with experimental UV-VIS result. The global reactivity parameters are calculated using the energies of frontier molecular orbitals also disclosed that the compound is more stable might be due to hydrogen bonding network. Experimental and molecular docking studies indicated that this compound has anti-bacterial and anti-diabetic properties. The binding affinity of this compound against the multidrug efflux pump subunit AcrB OS=Escherichia coli (strain K12) and Human Pancreatic Alpha-Amylase is -9.2 and -10.00 kcal/mol which are higher than the control drugs. Pi-Pi, Pi-anaion, amide-pi and pi-alkyl bonds play key role in drug-protein complexes.

  10. Synthesis, morphological control, and antibacterial properties of hollow/solid Ag2S/Ag heterodimers

    KAUST Repository

    Pang, Maolin

    2010-08-11

    Ag2S and Ag are important functional materials that have received considerable research interest in recent years. In this work, we develop a solution-based synthetic method to combine these two materials into hollow/solid Ag2S/Ag heterodimers at room temperature. Starting from monodisperse Cu2O solid spheres, CuS hollow spheres can be converted from Cu2O through a modified Kirkendall process, and the obtained CuS can then be used as a solid precursor for preparation of the Ag2S/Ag heterodimers through ion exchange and photo-assisted reduction. We have found that formation of the Ag2S/Ag heterodimers is instantaneous, and the size of Ag nanocrystals on the hollow spheres of Ag2S can be controlled by changing the concentration and power of reducing agents in the synthesis. The growth of Ag nanoparticles on hollow spheres of Ag2S in the dimers is along the [111] direction of the silver crystal; the light absorption properties have also been investigated. Furthermore, coupling or tripling of Ag2S/Ag heterodimers into dumbbell-like trimers ((Ag 2S)2/Ag, linear) and triangular tetramers ((Ag 2S)3/Ag, coplanar) can also be attained at 60°C by adding the bidentate ligand ethylenediamine as a cross-linking agent. To test the applicability of this highly asymmetric dipolar composite, photocatalytic inactivation of Escherichia coli K-12 in the presence of the as-prepared Ag 2S/Ag heterodimers has been carried out under UV irradiation. The added Ag2S/Ag heterodimers show good chemical stability under prolonged UV irradiation, and no appreciable solid dissolution is found. Possible mechanisms regarding the enhanced antibacterial activity have also been addressed. © 2010 American Chemical Society.

  11. Synthesis and investigation of physico-chemical, antibacterial, biomymetic properties of silver and zinc containing hydroxyapatite

    Science.gov (United States)

    Zhuk, Ilya; Rasskazova, Lyudmila; Korotchenko, Natalia; Kozik, Vladimir; Kurzina, Irina

    2017-11-01

    In the work we carried out microwave synthesis of modified hydroxyapatites (HA) with different content of ions. A solid solution based on HA remains a single-phase sample when the calcium ions are substituted by silver and zinc ions up to 5 % by weight (0.5 mole fraction). The microstructure parameters, morphology and the particle powders size were studied by X-ray diffraction analysis, IR spectroscopy, and scanning electron microscopy (SEM). It is shown that the modification of HA by silver (AgHA) and zinc (ZnHA) ions increases the size of its particles, the degree of crystallinity, and the pore sizes of the samples while reducing their specific surface and uniformity of their forms. Elemental analysis and distribution of elements over the surface of HA, AgHA, and ZnHA powders were performed by X-ray spectral microanalysis (RSMA). The ratio of Ca/P is within the range of 1.66-1.77 and corresponds to the ratio of Ca/P in stoichiometric HA and the HA entering bone tissue. The ability of AgHA- and ZnHA-substrates to form on their surface a calcium-phosphate layer from the simulated body fluid (SBF) at 37 °C is determined. This ability decreases in the order: in ZnHA it is less than in AgHA, but greater than in HA. The antibacterial activity of the samples was analyzed. The AgHA sample has both bactericidal and persistent bacteriostatic properties in the case of direct contact with Escherichia coli cells.

  12. Synthesis and spectral characterization of silver/magnesium co-substituted hydroxyapatite for biomedical applications

    Science.gov (United States)

    Gopi, D.; Shinyjoy, E.; Kavitha, L.

    2014-06-01

    The present work is aimed at the synthesis of antibacterial and bioactive silver/magnesium co-substituted hydroxyapatite (Ag/Mg-HAP) powders. For this purpose, firstly, different concentrations (0.5, 1.5, 2.5 wt.%) of silver substituted HAP (Ag-HAP) powders were prepared by ultrasonic irradiation technique and were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDAX). Secondly, magnesium (Mg) is co-substituted as secondary material into Ag-HAP to offset the potential cytotoxicity of Ag, as higher concentration of Ag is toxic. The antibacterial activity of as-synthesized powders was evaluated by Escherichia coli (E. coli) and was found to be effectively high against bacterial colonization. Also, the in vitro cell-material interaction is evaluated with human osteosarcoma MG63 (HOS MG63) cells for cell proliferation. The results showed the evidence of cytotoxic effects of the higher concentration of Ag-HAP characterized by poor cellular viability whereas, Ag/Mg-HAP showed better cell viability indicating that co-substitution of Mg in Ag-HAP effectively offset the negative effects of Ag and improve performance compared with pure HAP. Thus, the as synthesized Ag/Mg-HAP will serve as a better candidate for biomedical applications with good antibacterial property and bone bonding ability.

  13. Characterization of antibacterial polyethersulfone membranes using the respiration activity monitoring system (RAMOS)

    NARCIS (Netherlands)

    Kochan, J.; Scheidle, M.; Erkel, J. van; Bikel, M.; Büchs, J.; Wong, J.E.; Melin, T.; Wessling, M.

    2012-01-01

    Membranes with antibacterial properties were developed using surface modification of polyethersulfone ultrafiltration membranes. Three different modification strategies using polyelectrolyte layer-by-layer (LbL) technique are described. The first strategy relying on the intrinsic antibacterial

  14. Silver(I) complexes of N-methylbenzothiazole-2-thione: Synthesis, structures and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Aslanidis, P., E-mail: aslanidi@chem.auth.gr [Laboratory of Inorganic Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki (Greece); Hatzidimitriou, A.G.; Andreadou, E.G. [Laboratory of Inorganic Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki (Greece); Pantazaki, A.A., E-mail: natasa@chem.auth.gr [Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki (Greece); Voulgarakis, N. [Department of Logistics, Alexander Technological Educational Institute, GR-60100 Katerini (Greece)

    2015-05-01

    Three silver(I) complexes containing N-methylbenzothiazole-2-thione (mbtt) have been prepared and structurally characterized by X-ray single-crystal analysis. Silver(I) nitrate, and silver(I) triflate react with mbtt to give homoleptic complexes of formula [(mbtt){sub 2}Ag(μ-mbtt){sub 2}Ag(mbtt){sub 2}](NO{sub 3}){sub 2} (1) and [Ag(mbtt){sub 3}](CF{sub 3}SO{sub 3}) (2) respectively, while silver(I) chloride gives the binuclear halide-bridged [(mbtt){sub 2}Ag(μ{sub 2}-Cl){sub 2}Ag(mbtt){sub 2}] (3). In the binuclear complex 1 the two metal ions, separated by 3.73 Å from each other, are doubly bridged by the exocyclic S-atoms of two mbtt ligands, with the tetrahedral environment around each silver ion being completed by the S-atoms of two terminally bonded mbtt units. Compound 2 is mononuclear with the metal ion surrounded by the exocyclic S-atoms of three mbtt ligands in a nearly ideal trigonal planar arrangement. The new complexes showed significant in vitro antibacterial activity against certain Gram-positive and Gram-negative bacterial strains.

  15. Surfactant free microwave assisted synthesis of ZnO microspheres: Study of their antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Shinde, Vidula V. [Thin Film Materials Laboratory, Department of Physics, Shivaji University, Kolhapur 416004, M.S. (India); Dalavi, Dhanaji S. [KIT' s College of Engineering, Gokul Shirgaon, Kolhapur (India); Mali, Sawanta S.; Hong, Chang K. [Department of Advanced Chemical Engineering, Chonnam National University, Gwangju 500-757 (Korea, Republic of); Kim, Jin H. [Department of Materials Science and Engineering, Chonnam National University, 300 Yong bong-Dong, Gwangju 500-757 (Korea, Republic of); Patil, Pramod S., E-mail: patilps_2000@yahoo.com [Thin Film Materials Laboratory, Department of Physics, Shivaji University, Kolhapur 416004, M.S. (India)

    2014-07-01

    A well-organized zinc oxide microspheres (ZnO-MS) structure has been synthesized with the aid of microwave process simply by tuning the reaction time and microwave power. Scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET) measurements, X-ray diffractometer (XRD), UV–vis spectroscopy, FT-Raman spectrometer and X-ray photoelectron spectrometer (XPS) are employed to characterize and analyze the ZnO-MS powder annealed at 400 °C for 1 h. The SEM images demonstrate the formation of dense microspheres with lateral size around 1.5 μm which consists of ultrathin nanoplates with a typical thickness of ~20 nm, and indicates a high lateral aspect ratio. The XRD spectra indicate the polycrystalline nature having hexagonal crystal structure. The surface elemental composition of ZnO-MS was analyzed by XPS studies. Antibacterial activity of ZnO-MS was evaluated against Staphylococcus aureus (S. aureus) Gram-positive and Escherichia coli (E. coli) Gram-negative microorganisms.

  16. Characterization of green synthesized nano-formulation (ZnO-A. vera) and their antibacterial activity against pathogens.

    Science.gov (United States)

    Qian, Yiguang; Yao, Jun; Russel, Mohammad; Chen, Ke; Wang, Xiaoyu

    2015-03-01

    The application of nanotechnology in medicine has recently been a breakthrough in therapeutic drugs formulation. This paper presents the structural and optical characterization of a new green nano-formulation (ZnO-Aloe vera) with considerable antibacterial activity against pathogenic bacteria. Its particle structure, size and morphology were characterized by XRD, TEM and SEM. And optical absorption spectra and photoluminescence were measured synchronously. Their antibacterial activity against Escherichia coli and Staphylococcus aureus was also investigated using thermokinetic profiling and agar well diffusion method. The nano-formulation is spherical shape and hexagonal with a particle size ranging from 25 to 65 nm as well as an increased crystallite size of 49 nm. For antibacterial activity, the maximum inhibition zones of ZnO and ZnO+A. vera are 18.33 and 26.45 mm for E. coli, 22.11 and 28.12 mm for S. aureus (p<0.05). Considering Pmax, Qt and k, ZnO+A. vera nano-formulation has a significant (p < 0.05) antibacterial effect against S. aureus almost at all concentration and against E. coli at 15 and 25mg/L. ZnO+A. vera nano-formulation is much more toxic against S. aureus than E. coli, with an IC50 of 13.12 mg/L and 21.31 mg/L, respectively. The overall results reveal that the ZnO-A. vera nano-formulation has good surface energy, crystallinity, transmission, and enriched antibacterial activities. Their antibacterial properties are possibly relevant to particle size, microstructural ionization, the crystal formation and the Gram property of pathogens. This ZnO-A. vera nano-formulation could be utilized effectively as a spectral and significant antibacterial agent for pathogens in future medical and environmental concerns. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Synthesis and characterization of new ionic liquids

    International Nuclear Information System (INIS)

    Oliveira, L.M.C. de; Mattedi, S.; Boaventura, J.S.; Iglesias, M.; Universidad de Santiago de Compostela

    2010-01-01

    In recent years, ionic liquids have been highlighted for its potential in various industrial applications. Among them, the salts of Broensted has a promising profile for the low toxicity, low cost and simple synthesis. This paper presents the synthesis and characterization of new salts of Bronsted with branched (lactate) or large chain anions (oleate) for future use as additives promoters of proton conductivity in fuel cells of ethanol. Experimental data were measured for density, sound velocity and conductivity of pure ionic liquids and mixtures. The density decreases linearly with increasing temperature, and sound velocity shows a similar trend, but not linear. The conductivity increases according to the Arrhenius model with activation energy less than 10 J/mol. Tests NMR, FTIR and TGA confirm ionic structure and thermal stability up to 165 deg C. (author)

  18. Lactones 46. Synthesis, antifeedant and antibacterial activity of γ-lactones with a p-methoxyphenyl substituent.

    Science.gov (United States)

    Mazur, Marcelina; Skrobiszewski, Andrzej; Gładkowski, Witold; Podkowik, Magdalena; Bania, Jacek; Nawrot, Jan; Klejdysz, Tomasz; Wawrzeńczyk, Czesław

    2016-03-01

    Lactones are well known for their biological activity. Grosheimin and repin are potent deterrents against storage pests. The unsaturated lactones have exhibited a wide spectrum of antibacterial activity. In our study we focused on the synthesis and evaluation of the biological activity of anisaldehyde derivatives containing lactone function. Four new lactones were synthesized in one-step reductive dehalogenation or dehydrohalogenation reactions. These compounds, together with halolactones synthesized earlier, were tested for their antifeedant activity towards Sitophilus granaries, Trogoderma granarium and Tribolium confusum. The results of the tests showed that the highest activity, comparable with that of azadirachtin, towards all tested pests (total coefficient of deterrence 143.3-183.9) was observed for lactone with a vinyl substituent. The antibacterial activity of these compounds was also evaluated. The most potent lactone was active towards gram-positive bacteria strains. The results of biological tests showed that halogen atom removal significantly increased the antifeedant properties of γ-lactones with a p-methoxyphenyl substituent. Unsaturated lactones are most promising in the context of their possible industrial application as crop protection agents. Further structural modifications of lactones with aromatic rings are needed to find important structural factors increasing the antibacterial activity. © 2015 Society of Chemical Industry.

  19. Preparation and characterization of uniform-sized chitosan/silver microspheres with antibacterial activities

    Energy Technology Data Exchange (ETDEWEB)

    An, Jing; Ji, Zhenxing; Wang, Desong, E-mail: dswang06@126.com; Luo, Qingzhi; Li, Xueyan

    2014-03-01

    The chitosan/silver microspheres (CAgMs), which possess effective inhibitory on microorganisms, were prepared by an inverse-emulsification cross-linking method using CS/Ag sol as dispersed phase, whiteruss as continuous phase, and glutaraldehyde as crosslinking agent. The size and shape of CAgMs, greatly affecting their antibacterial activities, were controlled by varying the concentrations of cross-linking agent, emulsifier and CS/Ag colloid. The preparation conditions for obtaining uniform-sized microspheres were optimized. The morphology of CAgMs was characterized by scanning electron microscopy (SEM) and laser particle size analysis. The spherical CAgMs with smooth surface in the mean size of ca. 5 μm exhibited a narrow particle size distribution. Energy Dispersive X-ray spectroscopy (EDX) revealed the elemental composition of the microspheres. Transmission electron micrographs (TEM) and Fourier transform infrared spectroscopy (FTIR) of the microspheres confirmed the formation of silver nanoparticles (AgNPs). The X-ray diffraction (XRD) patterns and UV–Visible diffuse reflectance spectroscopy (UV–vis DRS) of the sample showed that AgNPs with the diameter no more than 20 nm were face-centered cubic crystallites. X-ray photoelectron spectroscopy (XPS) proved that Ag-O bond existed in the microspheres. Thermogravimetric analysis (TGA) showed that the starting decomposition temperature of CAgMs (ca. 260 °C) was much higher than that of CS (ca. 160 °C), suggesting that the as-prepared CAgMs possessed better thermal stability than original CS did. Antimicrobial assays were performed using typical Gram bacteria and fungi. The inhibitory effect indicated that the as-prepared microspheres exerted a stronger antibacterial activity as the concentration of the AgNPs is increasing, and the microspheres in smaller size had much better antibacterial activity than those in the larger size. The antimicrobial mechanism of CAgMs was discussed. - Highlights: • CAgM was

  20. Facile synthesis of silver nanoparticles and its antibacterial activity against Escherichia coli and unknown bacteria on mobile phone touch surfaces/computer keyboards

    Science.gov (United States)

    Reddy, T. Ranjeth Kumar; Kim, Hyun-Joong

    2016-07-01

    In recent years, there has been significant interest in the development of novel metallic nanoparticles using various top-down and bottom-up synthesis techniques. Kenaf is a huge biomass product and a potential component for industrial applications. In this work, we investigated the green synthesis of silver nanoparticles (AgNPs) by using kenaf ( Hibiscus cannabinus) cellulose extract and sucrose, which act as stabilizing and reducing agents in solution. With this method, by changing the pH of the solution as a function of time, we studied the optical, morphological and antibacterial properties of the synthesized AgNPs. In addition, these nanoparticles were characterized by Ultraviolet-visible spectroscopy, transmission electron microscopy (TEM), field-emission scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy and energy-dispersive X-ray spectroscopy (EDX). As the pH of the solution varies, the surface plasmon resonance peak also varies. A fast rate of reaction at pH 10 compared with that at pH 5 was identified. TEM micrographs confirm that the shapes of the particles are spherical and polygonal. Furthermore, the average size of the nanoparticles synthesized at pH 5, pH 8 and pH 10 is 40.26, 28.57 and 24.57 nm, respectively. The structure of the synthesized AgNPs was identified as face-centered cubic (fcc) by XRD. The compositional analysis was determined by EDX. FTIR confirms that the kenaf cellulose extract and sucrose act as stabilizing and reducing agents for the silver nanoparticles. Meanwhile, these AgNPs exhibited size-dependent antibacterial activity against Escherichia coli ( E. coli) and two other unknown bacteria from mobile phone screens and computer keyboard surfaces.

  1. Synthesis and Antibacterial Evaluation of New N-acylhydrazone Derivatives from Dehydroabietic Acid

    Directory of Open Access Journals (Sweden)

    Chenhai Gu

    2012-04-01

    Full Text Available A series of new N-acylhydrazone derivatives were synthesized in good yields through the reactions of dehydroabietic acid hydrazide with a variety of substituted arylaldehydes. The structures of the synthesized compounds were confirmed by IR, 1H- and 13C-NMR, ESI-MS, elemental analysis and single crystal X-ray diffraction. From the crystal structure of compound 4l, the C=N double bonds of these N-acylhydrazones showed (E-configuration, while the NMR data of compounds 4a–q indicated the existence of two rotamers for each compound in solution. The target compounds were evaluated for their antibacterial activities against four microbial strains. The result suggested that several compounds exhibited pronounced antibacterial activities. Particularly, compound 4p exhibited good antibacterial activity against Staphylococcus aureus and Bacillus subtilis comparable to positive control. The possible antibacterial metabolism and the strategy for further optimization of this compound were also discussed.

  2. Synthesis and Antibacterial Evaluation of Novel 3-Substituted Ocotillol-Type Derivatives as Leads.

    Science.gov (United States)

    Bi, Yi; Liu, Xian-Xuan; Zhang, Heng-Yuan; Yang, Xiao; Liu, Ze-Yun; Lu, Jing; Lewis, Peter John; Wang, Chong-Zhi; Xu, Jin-Yi; Meng, Qing-Guo; Ma, Cong; Yuan, Chun-Su

    2017-04-07

    Due to the rapidly growing bacterial antibiotic-resistance and the scarcity of novel agents in development, bacterial infection is still a global problem. Therefore, new types of antibacterial agents, which are effective both alone and in combination with traditional antibiotics, are urgently needed. In this paper, a series of antibacterial ocotillol-type C-24 epimers modified from natural 20( S )-protopanaxadiol were synthesized and evaluated for their antibacterial activity. According to the screening results of Gram-positive bacteria ( B. subtilis 168 and MRSA USA300) and Gram-negative bacteria ( P. aer PAO1 and A. baum ATCC19606) in vitro, the derivatives exhibited good antibacterial activity, particularly against Gram-positive bacteria with an minimum inhibitory concentrations (MIC) value of 2-16 µg/mL. The subsequent synergistic antibacterial assay showed that derivatives 5c and 6c enhanced the susceptibility of B. subtilis 168 and MRSA USA300 to chloramphenicol (CHL) and kanamycin (KAN) (FICI resistant pathogens MRSA USA300, which could improve the ability of KAN and CHL to exhibit antibacterial activity at much lower concentrations with reduced toxicity.

  3. Synthesis, structural characterisation and antibacterial activity of Ag{sup +}-doped fluorapatite nanomaterials prepared by neutralization method

    Energy Technology Data Exchange (ETDEWEB)

    Stanić, Vojislav, E-mail: voyo@vinca.rs [University of Belgrade, Vinča Institute of Nuclear Sciences, P.O. Box 522, 11001 Belgrade (Serbia); Radosavljević-Mihajlović, Ana S. [University of Belgrade, Vinča Institute of Nuclear Sciences, P.O. Box 522, 11001 Belgrade (Serbia); Živković-Radovanović, Vukosava [University of Belgrade, Faculty of Chemistry, P.O. Box 51, 11158 Belgrade (Serbia); Nastasijević, Branislav; Marinović-Cincović, Milena; Marković, Jelena P.; Budimir, Milica D. [University of Belgrade, Vinča Institute of Nuclear Sciences, P.O. Box 522, 11001 Belgrade (Serbia)

    2015-05-15

    Graphical abstract: - Highlights: • The neutralization method has been used for synthesis of silver-doped fluorapatite powders. • Particles of silver-doped fluorapatite samples are of nano size and homogenous in composition. • The Ag{sup +}-doped fluorapatite samples showed antibacterial effect against Kllebsiela pneumoniae, Staphylococcus aureus and Micrococcus luteus. • AFM studies showed that silver-doped sample causes considerable morphological changes of tested bacterial cells. - Abstract: Silver doped fluorapatite nanopowders were synthesised by neutralization method, which consists of dissolving Ag{sub 2}O in solution of HF and H{sub 3}PO{sub 4} and addition to suspension of Ca(OH){sub 2}. The powder XRD, SEM and FTIR studies indicated the formation of a fluorapatite nanomaterials with average length of the particles is about 80 nm and a width of about 15 nm. The FTIR studies show that carbonate content in samples is very small and carbonte ions substitute both phosphate and hydroxyl groups in the crystal structure of samples, forming AB-type fluorapatite. Antibacterial studies have demonstrated that all Ag{sup +}-doped fluorapatite samples exhibit bactericidal effect against pathogens: Staphylococcus aureus, Micrococcus luteus and Kllebsiela pneumoniae. Antibacterial activity increased with the increase of Ag{sup +} in the samples. The atomic force microscopy studies revealed extensive damage to the bacterial cell envelops in the presence of Ag{sup +}-doped fluorapatite particles which may lead to their death. The synthesized Ag{sup +}-doped fluorapatite nanomaterials are promising as antibacterial biomaterials in orthopedics and dentistry.

  4. Enantioselective Total Synthesis of Antibiotic CJ-16,264, Synthesis and Biological Evaluation of Designed Analogues, and Discovery of Highly Potent and Simpler Antibacterial Agents.

    Science.gov (United States)

    Nicolaou, K C; Pulukuri, Kiran Kumar; Rigol, Stephan; Buchman, Marek; Shah, Akshay A; Cen, Nicholas; McCurry, Megan D; Beabout, Kathryn; Shamoo, Yousif

    2017-11-08

    An improved and enantioselective total synthesis of antibiotic CJ-16,264 through a practical kinetic resolution and an iodolactonization reaction to form the iodo pyrrolizidinone fragment of the molecule is described. A series of racemic and enantiopure analogues of CJ-16,264 was designed and synthesized through the developed synthetic technologies and tested against drug-resistant bacterial strains. These studies led to interesting structure-activity relationships and the identification of a number of simpler, and yet equipotent, or even more potent, antibacterial agents than the natural product, thereby setting the foundation for further investigations in the quest for new anti-infective drugs.

  5. Graphene optoelectronics synthesis, characterization, properties, and applications

    CERN Document Server

    bin M Yusoff, Abdul Rashid

    2014-01-01

    This first book on emerging applications for this innovative material gives an up-to-date account of the many opportunities graphene offers high-end optoelectronics.The text focuses on potential as well as already realized applications, discussing metallic and passive components, such as transparent conductors and smart windows, as well as high-frequency devices, spintronics, photonics, and terahertz devices. Also included are sections on the fundamental properties, synthesis, and characterization of graphene. With its unique coverage, this book will be welcomed by materials scientists, solid-

  6. Design, synthesis, characterization and study of novel conjugated polymers

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Wu [Iowa State Univ., Ames, IA (United States)

    1997-06-24

    After introducing the subject of conjugated polymers, the thesis has three sections each containing a literature survey, results and discussion, conclusions, and experimental methods on the following: synthesis, characterization of electroluminescent polymers containing conjugated aryl, olefinic, thiophene and acetylenic units and their studies for use in light-emitting diodes; synthesis, characterization and study of conjugated polymers containing silole unit in the main chain; and synthesis, characterization and study of silicon-bridged and butadiene-linked polythiophenes.

  7. Egg extract of apple snail for eco-friendly synthesis of silver nanoparticles and their antibacterial activity.

    Science.gov (United States)

    Janthima, Ratima; Khamhaengpol, Arunrat; Siri, Sineenat

    2018-03-01

    Green synthesis of silver nanoparticles (AgNPs) provides the alternative method with cost effectiveness and the eco-friendly process by using natural biomolecules as reducing and stabilizing agents. Alternative to the most studies of plant extracts, this work demonstrated a use of egg extract of apple snail (Pomacea canaliculata) for an eco-friendly production of AgNPs. The extract contained at least six proteins with the molecular weight in a range of 24-65 kDa that exhibited the reducing activity. The dispersive AgNPs were produced in the reaction containing only the extract and silver nitrate, as determined by the characteristic surface plasmon resonance peak of silver at 412 nm. The synthesized AgNPs were spherical with the average diameter of 9.0 ± 5.9 nm. The X-ray diffraction pattern and selected area electron diffraction (SAED) analyses confirmed the face-cubic centre (fcc) unit cell structure of AgNPs. The synthesized AgNPs exhibited the antibacterial activity against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Results of this work clearly showed the potential use of the egg extract of apple snail for a green synthesis of small size AgNPs exhibiting the antibacterial activity.

  8. Synthesis and Characterization of Novel Quaternary Thioaluminogermanates

    KAUST Repository

    Al-Bloushi, Mohammed

    2013-05-01

    Metal chalcogenides form an important class of inorganic materials, which include several technologically important applications. The design of metal chlcogenides is of technological interest and has encouraged recent research into moderate temperature solid-state synthetic methods for the single crystal growth of new materials. The aim of this project is the investigation and development of synthetic methodology for the synthesis of novel metal chlcogenides. The new inorganic compounds of the type “M(AlS2)(GeS2)” (M = Na and K) are new metal-chalcogenides, synthesized by the classical solid state approach. The characterization of these compounds was carried out by Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), Single crystal and powder X-ray diffraction, solid state Nuclear Magnetic Resonance (NMR), Ultraviolet-visible (UV-VIS), Infrared (IR) and Raman spectroscopy. These theses study the synthesis of metal chalcogenides through the use of standard chemical techniques. The systematic studies demonstrate the effect of the reactants ratio and reaction temperature on the synthesis and growth of the single crystals. Metal chalcogenides have several potential applications in gas separation, ion exchange, environmental remediation, and energy storage. Especially, the ion exchange materials have found\\tpossible applications in waste-water treatment, water softening, metal separation, and production of high purity water.

  9. Characterization and antibacterial activity of silver exchanged regenerated NaY zeolite from surfactant-modified NaY zeolite

    Energy Technology Data Exchange (ETDEWEB)

    Salim, Mashitah Mad; Malek, Nik Ahmad Nizam Nik, E-mail: niknizam@fbb.utm.my

    2016-02-01

    The antibacterial activity of regenerated NaY zeolite (thermal treatment from cetyltrimethyl ammonium bromide (CTAB)-modified NaY zeolite and pretreatment with Na ions) loaded with silver ions were examined using the broth dilution minimum inhibitory concentration (MIC) method against Escherichia coli (E. coli ATCC 11229) and Staphylococcus aureus (S. aureus ATCC 6538). X-ray diffraction (XRD), attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and chemical elemental analyses were used to characterize the regenerated NaY and AgY zeolites. The XRD patterns indicated that the calcination and addition of silver ions on regenerated NaY zeolite did not affect the structure of the regenerated NaY zeolite as the characteristic peaks of the NaY zeolite were retained, and no new peaks were observed. The regenerated AgY zeolite showed good antibacterial activity against both bacteria strains in distilled water, and the antibacterial activity of the samples increased with increasing Ag loaded on the regenerated AgY zeolite; the regenerated AgY zeolite was more effective against E. coli than S. aureus. However, the antibacterial activity of the regenerated AgY was not effective in saline solution for both bacteria. The study showed that CTAB-modified NaY zeolite materials could be regenerated to NaY zeolite using thermal treatment (550 °C, 5 h) and this material has excellent performance as an antibacterial agent after silver ions loading. - Highlights: • Thermal treatment was used to regenerate surfactant modified zeolite. • The regenerated NaY zeolite formed was added with different silver loadings. • Regenerated AgY zeolite was tested for antibacterial activity on E. coli and S. aureus. • The antibacterial activity increased with increased of the amount of silver loadings. • The zeolite structure did not change with thermal and modification

  10. Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil

    Directory of Open Access Journals (Sweden)

    Tony Picoli

    2016-12-01

    Full Text Available ABSTRACT. Picoli T., Peter C.M., Hoffmann J.F., Latosinski G.S., Zani J.L., D’Ávila Vargas G., Hüber S. de O. & Fischer G. [Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil.] Caracterização química e ação antibacteriana de extrato de própolis marrom da região sul do Brasil. Revista Brasileira de Medicina Veterinária, 38(4:365-371, 2016. Programa de Pós-Graduação em Veterinária, Laboratório de Virologia e Imunologia, Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário Capão do Leão s/n, Capão do Leão, RS 96900-010, Brasil. E-mail: picolivet@gmail.com The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g, caffeic acid (0.17 mg/g and p-coumaric acid (3.39 mg/g.In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01. With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6. Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between

  11. Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds.

    Science.gov (United States)

    Carvalho, Aline de Souza; da Silva, Márcia Vanusa; Gomes, Francis Soares; Paiva, Patrícia Maria Guedes; Malafaia, Carolina Barbosa; da Silva, Tulio Diego; Vaz, Antônio Fernando de Melo; da Silva, Alexandre Gomes; Arruda, Isabel Renata de Souza; Napoleão, Thiago Henrique; Carneiro-da-Cunha, Maria das Graças; Correia, Maria Tereza dos Santos

    2015-04-01

    Apuleia leiocarpa is a tree found in Caatinga that has great value in the timber industry. Lectins are carbohydrate-binding proteins with several biotechnological applications. This study shows the isolation, characterization, and antibacterial activity of A. leiocarpa seed lectin (ApulSL). The lectin was chromatographically isolated from a crude extract (in 150 mM NaCl) by using a chitin column. ApulSL adsorbed to the matrix and was eluted using 1.0 M acetic acid. Native ApulSL was characterized as a 55.8-kDa acidic protein. SDS-PAGE showed three polypeptide bands, whereas two-dimensional electrophoresis revealed four spots. The peptides detected by MALDI TOF/TOF did not show sufficient homology (<30%) with the database proteins. Circular dichroism spectroscopy suggested a disordered conformational structure, and fluorescence spectrum showed the presence of tyrosine residues in the hydrophobic core. The hemagglutinating activity of ApulSL was present even after heating to 100 °C, was Mn(2+)-dependent, and inhibited by N-acetylglucosamine, D(-)-arabinose, and azocasein. ApulSL demonstrated bacteriostatic and bactericide effects on gram-positive and gram-negative species, being more effective against three varieties of Xanthomonas campestris (MIC ranging from 11.2 to 22.5 μg/mL and MBC of 22.5 μg/mL). The results of this study reinforce the importance of biochemical prospecting of Caatinga by revealing the antibacterial potential of ApulSL. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Identification and characterization of antibacterial compound(s) of cockroaches (Periplaneta americana).

    Science.gov (United States)

    Ali, Salwa Mansur; Siddiqui, Ruqaiyyah; Ong, Seng-Kai; Shah, Muhammad Raza; Anwar, Ayaz; Heard, Peter J; Khan, Naveed Ahmed

    2017-01-01

    Infectious diseases remain a significant threat to human health, contributing to more than 17 million deaths, annually. With the worsening trends of drug resistance, there is a need for newer and more powerful antimicrobial agents. We hypothesized that animals living in polluted environments are potential sources of antimicrobials. Under polluted milieus, organisms such as cockroaches encounter different types of microbes, including superbugs. Such creatures survive the onslaught of superbugs and are able to ward off disease by producing antimicrobial substances. Here, we characterized antibacterial properties in extracts of various body organs of cockroaches (Periplaneta americana) and showed potent antibacterial activity in crude brain extract against methicillin-resistant Staphylococcus aureus and neuropathogenic Escherichia coli K1. The size-exclusion spin columns revealed that the active compound(s) are less than 10 kDa in molecular mass. Using cytotoxicity assays, it was observed that pre-treatment of bacteria with lysates inhibited bacteria-mediated host cell cytotoxicity. Using spectra obtained with LC-MS on Agilent 1290 infinity liquid chromatograph, coupled with an Agilent 6460 triple quadruple mass spectrometer, tissues lysates were analysed. Among hundreds of compounds, only a few homologous compounds were identified that contained the isoquinoline group, chromene derivatives, thiazine groups, imidazoles, pyrrole-containing analogs, sulfonamides, furanones, and flavanones and known to possess broad-spectrum antimicrobial properties and anti-inflammatory, anti-tumour, and analgesic properties. Further identification, characterization, and functional studies using individual compounds can act as a breakthrough in developing novel therapeutics against various pathogens including superbugs.

  13. Synthesis of new antibacterial quaternary ammonium monomer for incorporation into CaP nanocomposite

    Science.gov (United States)

    Zhou, Chenchen; Weir, Michael D.; Zhang, Ke; Deng, Dongmei; Cheng, Lei; Xu, Hockin H. K.

    2013-01-01

    Objectives Composites are the principal material for tooth cavity restorations due to their esthetics and direct-filling capabilities. However, composites accumulate biofilms in vivo, and secondary caries due to biofilm acids is the main cause of restoration failure. The objectives of this study were to: (1) synthesize new antibacterial monomers; and (2) develop nanocomposite containing nanoparticles of amorphous calcium phosphate (NACP) and antibacterial monomer. Methods Two new antibacterial monomers were synthesized: dimethylaminohexane methacrylate (DMAHM) with a carbon chain length of 6, and dimethylaminododecyl methacrylate (DMADDM) with a chain length of 12. A spray-drying technique was used to make NACP. DMADDM was incorporated into NACP nanocomposite at mass fractions of 0%, 0.75%, 1.5%, 2.25% and 3%. A flexural test was used to measure composite strength and elastic modulus. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure viability, metabolic activity, and lactic acid production of biofilms on composites. Results The new DMAHM was more potent than a previous quaternary ammonium dimethacrylate (QADM). DMADDM was much more strongly antibacterial than DMAHM. The new DMADDM-NACP nanocomposite had strength similar to that of composite control (p > 0.1). At 3% DMADDM in the composite, the metabolic activity of adherent biofilms was reduced to 5% of that on composite control. Lactic acid production by biofilms on composite containing 3% DMADDM was reduced to only 1% of that on composite control. Biofilm colony-forming unit (CFU) counts on composite with 3% DMADDM were reduced by 2-3 orders of magnitude. Significance New antibacterial monomers were synthesized, and the carbon chain length had a strong effect on antibacterial efficacy. The new DMADDM-NACP nanocomposite possessed potent anti-biofilm activity without compromising load-bearing properties, and is promising for antibacterial and remineralizing dental

  14. Synthesis and characterization of peapods and DWCNTs

    Energy Technology Data Exchange (ETDEWEB)

    Anis, B.; Kuntscher, C.A. [Experimentalphysik 2, Universitaet Augsburg, 86195 Augsburg (Germany); Fischer, M.; Schreck, M. [Experimentalphysik 4, Universitaet Augsburg, 86195 Augsburg (Germany); Haubner, K.; Dunsch, L. [Center of Spectroelectrochemistry, IFW Dresden, P.O. Box 270116, 01171 Dresden (Germany)

    2012-12-15

    We report the synthesis and characterization of C{sub 60} rate at SWCNT peapods and double-walled carbon nanotubes (DWCNTs) derived from the peapods. Single-walled carbon nanotubes (SWCNTs), C{sub 60} rate at SWCNT peapods, and DWCNTs were characterized by Raman and optical spectroscopy. The radial breathing modes (RBMs) of the tubes in C{sub 60} rate at SWCNT peapods are shifted to higher energies compared to the RBMs in SWCNTs, while in the case of DWCNTs the RBMs related to the outer tubes are shifted to lower energies compared to SWCNTs. A similar trend is observed for the absorption bands. These results suggest that the filling of the SWCNTs with C{sub 60} molecules decreases the average diameter of the electron cloud around the tubes, whereas the filling with an inner tube increases it. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  15. Eco-friendly biosynthesis and characterization of silver nanoparticles using Tinospora cordifolia (Thunb. Miers and evaluate its antibacterial, antioxidant potential

    Directory of Open Access Journals (Sweden)

    Kandasamy Selvam

    2017-01-01

    Full Text Available The present study reports an eco-friendly, rapid and easy method for synthesis of silver nanoparticles (AgNPs using Tinospora cordifolia as a reducing and capping agent. The different factor such as silver nitrate (AgNO3 concentration, fresh weight of T. cordifolia leaf, incubation time, and pH affecting silver reduction was investigated using Response surface methodology based Box–Behnken design (BBD. The optimum conditions were AgNO3 (1.25 mM, incubation time (15 h, Temperature (45 °C and pH (4.5. T. cordifolia leaf extract can reduces silver ions into AgNPs within 30 min after heating the reaction mixture (60 °C as indicated by the developed reddish brown color. The UV-Vis spectrum of AgNPs revealed a characteristic surface plasmon resonance (SPR peak at 430 nm. AgNPs were characterized X-ray diffraction (XRD revealed their crystalline nature and their average size of nanoparticles was 30 nm as determined by using Scherrer's equation. Fourier transform infrared (FTIR spectroscopy affirmed the role of T. cordifolia leaf extract as a reducing and capping agent of silver ions. Scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDS showed spherical shaped and confirming presence of elemental silver. The synthesized AgNPs was found higher antioxidant activity than plant extract by dot plot assay. In addition, antibacterial activity against Staphylococcus sp. (NCBI-Accession: KC688883.1 and Klebsiella sp. (NCBI-Accession: KF649832.1, showed maximum zone of inhibition of 13 mm and 12.3 mm, respectively, at 10 μg/mL of AgNPs. From the results it is suggested that the synthesized AgNPs showed higher antioxidant and antibacterial activity than the plant extract, thus signification of the present study is the production of biomedical products.

  16. Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties.

    Science.gov (United States)

    Stankic, Slavica; Suman, Sneha; Haque, Francia; Vidic, Jasmina

    2016-10-24

    Th antibacterial activity of metal oxide nanoparticles has received marked global attention as they can be specifically synthesized to exhibit significant toxicity to bacteria. The importance of their application as antibacterial agents is evident keeping in mind the limited range and effectiveness of antibiotics, on one hand, and the plethora of metal oxides, on the other, along with the propensity of nanoparticles to induce resistance being much lower than that of antibiotics. Effective inhibition against a wide range of bacteria is well known for several nano oxides consisting of one metal (Fe 3 O 4 , TiO 2 , CuO, ZnO), whereas, research in the field of multi-metal oxides still demands extensive exploration. This is understandable given that the relationship between physicochemical properties and biological activity seems to be complex and difficult to generalize even for metal oxide nanoparticles consisting of only one metal component. Also, despite the broad scope that metal oxide nanoparticles have as antibacterial agents, there arise problems in practical applications taking into account the cytotoxic effects. In this respect, the consideration of polymetallic oxides for biological applications becomes even greater since these can provide synergetic effects and unify the best physicochemical properties of their components. For instance, strong antibacterial efficiency specific of one metal oxide can be complemented by non-cytotoxicity of another. This review presents the main methods and technological advances in fabrication of nanostructured metal oxides with a particular emphasis to multi-metal oxide nanoparticles, their antibacterial effects and cytotoxicity.

  17. Synthesis, Transfer, and Characterization of Nanoscale 2-Dimensional Materials

    Science.gov (United States)

    2015-09-01

    stack is floating on top of the DDI water surface. Instead of removing the stack with the target substrate, a graphene/copper foil substrate (graphene...demonstrated the synthesis of graphene, hexagonal boron nitride, and bismuth telluride using chemical and physical vapor deposition techniques. Making...for material synthesis, transfer, and characterization. 15. SUBJECT TERMS graphene, hexagonal boron nitride, bismuth telluride, synthesis, transfer

  18. Design, synthesis, characterization and antibacterial and antifungal activity of a new 2-{(E-[(4-aminophenylimino]-methyl}-4,6-dichlorophenol and its complexes with Co(II, Ni(II, Cu(II and Zn(II: An experimental and DFT study

    Directory of Open Access Journals (Sweden)

    Bakirdere Emine Gulhan

    2016-01-01

    Full Text Available In this study, the complexes of Co (II, Ni (II, Cu (II and Zn (II with 2-(E-(4-aminophenyliminomethyl-4,6-dichlorophenol were prepared and characterized by physical, spectral and analytical data. The metal: ligand stoichiometric ratio is 1:2 in all the complexes. The results suggested that the Schiff bases are coordinated to the metal ions through the phenolic oxygens and azomethine nitrogen to give mononuclear complexes. Their structures were elucidated on the basis of elemental analysis, IR, 1H and 13C NMR spectra, UV-VIS, magnetic susceptibility measurements and thermogravimetric analyses. Both the antibacterial and antifungal activities and MIC values of compounds were reported. Among the tested compounds, the most effective compound providing a MIC value of 64 μg/mL is Zn(L2 against C. tropicalis and B. subtilis. The theoretically optimized geometries of complexes have tetrahedral structures. The computed stretching frequencies of C=N, C-O and N-H bonds were found to be in good agreement with experimental data. All calculated frequencies fall within about 5% of the experimental frequency regions.

  19. Preparation and characterization of uniform-sized chitosan/silver microspheres with antibacterial activities.

    Science.gov (United States)

    An, Jing; Ji, Zhenxing; Wang, Desong; Luo, Qingzhi; Li, Xueyan

    2014-03-01

    The chitosan/silver microspheres (CAgMs), which possess effective inhibitory on microorganisms, were prepared by an inverse-emulsification cross-linking method using CS/Ag sol as dispersed phase, whiteruss as continuous phase, and glutaraldehyde as crosslinking agent. The size and shape of CAgMs, greatly affecting their antibacterial activities, were controlled by varying the concentrations of cross-linking agent, emulsifier and CS/Ag colloid. The preparation conditions for obtaining uniform-sized microspheres were optimized. The morphology of CAgMs was characterized by scanning electron microscopy (SEM) and laser particle size analysis. The spherical CAgMs with smooth surface in the mean size of ca. 5 μm exhibited a narrow particle size distribution. Energy Dispersive X-ray spectroscopy (EDX) revealed the elemental composition of the microspheres. Transmission electron micrographs (TEM) and Fourier transform infrared spectroscopy (FTIR) of the microspheres confirmed the formation of silver nanoparticles (AgNPs). The X-ray diffraction (XRD) patterns and UV-Visible diffuse reflectance spectroscopy (UV-vis DRS) of the sample showed that AgNPs with the diameter no more than 20 nm were face-centered cubic crystallites. X-ray photoelectron spectroscopy (XPS) proved that AgO bond existed in the microspheres. Thermogravimetric analysis (TGA) showed that the starting decomposition temperature of CAgMs (ca. 260°C) was much higher than that of CS (ca. 160°C), suggesting that the as-prepared CAgMs possessed better thermal stability than original CS did. Antimicrobial assays were performed using typical Gram bacteria and fungi. The inhibitory effect indicated that the as-prepared microspheres exerted a stronger antibacterial activity as the concentration of the AgNPs is increasing, and the microspheres in smaller size had much better antibacterial activity than those in the larger size. The antimicrobial mechanism of CAgMs was discussed. Copyright © 2013 Elsevier B.V. All

  20. Synthesis of new antibacterial composite coating for titanium based on highly ordered nanoporous silica and silver nanoparticles.

    Science.gov (United States)

    Massa, Miguel A; Covarrubias, Cristian; Bittner, Mauricio; Fuentevilla, Ignacio Andrés; Capetillo, Pavel; Von Marttens, Alfredo; Carvajal, Juan Carlos

    2014-12-01

    Infection is the most common factor that leads to dental titanium implant failure. Antibacterial implant surfaces based on nano-scale modifications of the titanium appear as an attractive strategy for control of peri-implantitis. In the present work, the preparation and antibacterial properties of a novel composite coating for titanium based on nanoporous silica and silver nanoparticles are presented. Starch-capped silver nanoparticles (AgNPs) were synthesized and then incorporated into sol-gel based solution system. The AgNP-doped nanoporous silica coatings were prepared on titanium surface using a combined sol-gel and evaporation-induced self-assembly (EISA) method. The coating nanostructure was characterized by XRD, SEM-EDX, and HR-TEM. Antibacterial activity was evaluated against Aggregatibacter actinomycetemcomitans, a representative pathogen of dental peri-implantitis. Colony-forming units (CFUs) were counted within the biofilm and at the planktonic state. Biofilm development was quantified using crystal violet staining and viability of adherent bacteria was confirmed with the Live/Dead fluorescence assay. Silica-based composite coating containing AgNPs (AgNP/NSC) was prepared on titanium surface by direct incorporation of AgNP suspension into the sol-gel system. The self-assembly technique enabled the spontaneous formation of a highly ordered nanoporosity in the coating structure, which is a desired property for osseointegration aspects of titanium implant surface. AgNP/NSC coating produces a strong antibacterial effect on titanium surface by not only killing the adherent bacteria but also reducing the extent of biofilm formation. Biofilm survival is reduced by more than 70% on the AgNP/NSC-modified titanium surface, compared to the control. This antibacterial effect was verified for up to 7 days of incubation. The long-term antibacterial activity exhibited by the nanostructured AgNP/NSC-titanium surface against A. actinomycetemcomitans suggests that this

  1. Fabrication and characterization of antibacterial nanoparticles supported on hierarchical hybrid substrates

    Science.gov (United States)

    Karumuri, Anil K.; Maleszewski, Adam A.; Oswal, Dhawal P.; Hostetler, Heather A.; Mukhopadhyay, Sharmila M.

    2014-04-01

    The effectiveness of many nanomaterial-based devices depends upon their available surface area. Isolated nanoparticles (NPs) can offer high-surface area, but are prone to environmental loss and pollution. Whereas those supported on solid substrates are limited by the specific surface area (SSA) of the support. The SSA limitation of traditional supports can be addressed by attaching NPs on specially designed hierarchical structures having unusually high SSA, thereby maximizing the nanomaterial advantage without the risks of using loose nano-powders. In this research, hierarchical structures were fabricated by grafting carbon nanotubes (CNT) on carbon and subsequently decorated with strongly attached silver nanoparticles (AgNP) via controlled reduction of silver salts in the presence of reducing and capping agents. Microstructure characterization revealed that along with other processing parameters, reduction temperature can be used to control NP morphology. For this substrate morphology, fine and uniformly dispersed AgNP were obtained at 60 °C, whereas significant particle coalescence and increase in particle size occurred at 80 °C. Mechanical durability of AgNP-CNT attachments on the substrate was tested in harsh ultrasonic conditions and found to be impressive, with no detectable AgNP loss even when the larger substrate begins to fail. The antibacterial effectiveness of these structures was tested in multiple testing modes against Gram-negative Escherichia coli ( E. coli, JM109). It was seen in each case that AgNP attached on CNT-grafted hierarchical substrates showed significantly higher reduction of E. coli compared to AgNP attached directly on the starting porous supports without CNT grafting. These results indicate that AgNP attached to hierarchal hybrid supports can lead to compact and powerful antibacterial devices for chemical-free disinfection devices of the future.

  2. Hybrid Drug Delivery Patches Based on Spherical Cellulose Nanocrystals and Colloid Titania—Synthesis and Antibacterial Properties

    Directory of Open Access Journals (Sweden)

    Olga L. Evdokimova

    2018-04-01

    Full Text Available Spherical cellulose nanocrystal-based hybrids grafted with titania nanoparticles were successfully produced for topical drug delivery. The conventional analytical filter paper was used as a precursor material for cellulose nanocrystals (CNC production. Cellulose nanocrystals were extracted via a simple and quick two-step process based on first the complexation with Cu(II solution in aqueous ammonia followed by acid hydrolysis with diluted H2SO4. Triclosan was selected as a model drug for complexation with titania and further introduction into the nanocellulose based composite. Obtained materials were characterized by a broad variety of microscopic, spectroscopic, and thermal analysis methods. The drug release studies showed long-term release profiles of triclosan from the titania based nanocomposite that agreed with Higuchi model. The bacterial susceptibility tests demonstrated that released triclosan retained its antibacterial activity against Escherichia coli and Staphylococcus aureus. It was found that a small amount of titania significantly improved the antibacterial activity of obtained nanocomposites, even without immobilization of model drug. Thus, the developed hybrid patches are highly promising candidates for potential application as antibacterial agents.

  3. Synthesis, characterization and antimicrobial activity of some novel benzimidazole derivatives.

    Science.gov (United States)

    Krishnanjaneyulu, Immadisetty Sri; Saravanan, Govindaraj; Vamsi, Janga; Supriya, Pamidipamula; Bhavana, Jarugula Udaya; Sunil Kumar, Mittineni Venkata

    2014-01-01

    A series of novel N-((1H-benzoimidazol-1-yl) methyl)-4-(1-phenyl-5-substituted-4, 5-dihydro-1-benzoimidazol-1-yl) methyl)-4-(1-phenyl-5-substituted-4, 5-dihydro-1H-pyrazol-3-yl) benzenamine were synthesized by treating various 1-(4-((1H-benzoimidazol-1-yl) methylamino) phenyl)-3-substitutedprop-2-en-1-one with phenyl hydrazine in the presence of sodium acetate through a simple ring closure reaction. The starting material, 1-(4-((1H-benzoimidazol-1-yl) methylamino) phenyl)-3-substitutedprop-2-en-1-one,-benzoimidazol-1-yl) methylamino) phenyl)-3-substitutedprop-2-en-1-one, was synthesized from o-phenylenediamine by a multistep synthesis. All the synthesized compounds were characterized by spectroscopic means and elemental analyses. The title compounds were investigated for in vitro antibacterial and antifungal properties against some human pathogenic microorganisms by employing the agar streak dilution method using Ciprofloxacin and Ketoconazole as standard drugs. All title compounds showed activity against the entire strains of microorganism. Structural activity relationship studies reveal that compounds possessing an electron-withdrawing group display better activity than the compounds containing electron-donating groups, whereas the unsubstituted derivatives display moderate activity. Based on the results obtained, N-((1H-benzoimidazol-1-yl) methyl)-4-(1-phenyl-5-(4-(trifluoromethyl) phenyl)-4,5-dihydro-1H-pyrazol-3-yl) benzenamine 5i was found to be very active compared with the rest of the compounds and standard drugs that were subjected to antimicrobial assay.

  4. Synthesis, characterization and antimicrobial activity of some novel benzimidazole derivatives

    Directory of Open Access Journals (Sweden)

    Immadisetty Sri Krishnanjaneyulu

    2014-01-01

    Full Text Available A series of novel N-((1H-benzoimidazol-1-yl methyl-4-(1-phenyl-5-substituted-4, 5-dihydro-1-benzoimidazol-1-yl methyl-4-(1-phenyl-5-substituted-4, 5-dihydro-1H-pyrazol-3-yl benzenamine were synthesized by treating various 1-(4-((1H-benzoimidazol-1-yl methylamino phenyl-3-substitutedprop-2-en-1-one with phenyl hydrazine in the presence of sodium acetate through a simple ring closure reaction. The starting material, 1-(4-((1H-benzoimidazol-1-yl methylamino phenyl-3-substitutedprop-2-en-1-one,-benzoimidazol-1-yl methylamino phenyl-3-substitutedprop-2-en-1-one, was synthesized from o-phenylenediamine by a multistep synthesis. All the synthesized compounds were characterized by spectroscopic means and elemental analyses. The title compounds were investigated for in vitro antibacterial and antifungal properties against some human pathogenic microorganisms by employing the agar streak dilution method using Ciprofloxacin and Ketoconazole as standard drugs. All title compounds showed activity against the entire strains of microorganism. Structural activity relationship studies reveal that compounds possessing an electron-withdrawing group display better activity than the compounds containing electron-donating groups, whereas the unsubstituted derivatives display moderate activity. Based on the results obtained, N-((1H-benzoimidazol-1-yl methyl-4-(1-phenyl-5-(4-(trifluoromethyl phenyl-4,5-dihydro-1H-pyrazol-3-yl benzenamine 5i was found to be very active compared with the rest of the compounds and standard drugs that were subjected to antimicrobial assay.

  5. Synthesis and characterization of zeolite L

    International Nuclear Information System (INIS)

    Ko, Yong Sig; Ahn, Wha Seung

    1999-01-01

    Substantial reduction in synthesis time was achieved for zeolite L crystallization by attempting a hydrothermal synthesis at elevated temperature of 443K in a Na + /K+ mixed alkali system. Pure zeolite L could be obtained from a gel with the molar composition 5.4K 2 O-5.7Na 2 O-Al 2 O 3 -30SiO 2 -500H 2 O after 24h. Zeolite L could be obtained in high purity at the optimum Na 2 O/(K 2 O+Na 2 O) ratio of around 0.5, while zeolite W was formed when the Na 2 O/(K 2 O+Na 2 O) ratio was more than 0.66. The crystalline zeolite L samples obtained were characterized by means of elemental chemical analysis, XRD, SEM, FTi.r. spectroscopy, and particle size analyzer. In addition, two probe reaction studies were conducted. In toluene alkylation, H-L catalyst showed high catalytic activity at the beginning, but was deactivated quickly probably due to one-dimensional pore structure being blocked by the coke formed. High amounts of trimethylbenzene or diethylbenzene were observed due to the large 12-membered ring pore structure of zeolite L. Pt/NaKL catalyst prepared showed a high conversion of n-hexane and high selectivity to benzene in n-hexane aromatization reaction

  6. Synthesis and characterization of innovative insulation materials

    Directory of Open Access Journals (Sweden)

    Skaropoulou Aggeliki

    2018-01-01

    Full Text Available Insulation elements are distinguished in inorganic fibrous and organic foamed materials. Foamed insulation materials are of great acceptance and use, but their major disadvantage is their flammability. In case of fire, they tend to transmit the flame producing toxic gases. In this paper, the synthesis and characterization of innovative inorganic insulation materials with properties competitive to commercial is presented. Their synthesis involves the mixing of inorganic raw material and water with reinforcing agent or/and foaming agent leading to the formation of a gel. Depending on raw materials nature, the insulation material is produced by freeze drying or ambient drying techniques of the gel. The raw material used are chemically benign and abundantly available materials, or industrial by-products and the final products are non-toxic and, in some cases, non-flammable. Their density and thermal conductivity was measured and found 0.02-0.06 g/cm3 and 0.03-0.04 W/mK, respectively.

  7. Green synthesis and characterization of graphene nanosheets

    Energy Technology Data Exchange (ETDEWEB)

    Tavakoli, Farnosh [School of Chemistry, College of Science, University of Tehran, Tehran (Iran, Islamic Republic of); Salavati-Niasari, Masoud, E-mail: salavati@kashanu.ac.ir [Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P. O. Box. 87317-51167, Islamic Republic of Iran (Iran, Islamic Republic of); Department of Inorganic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, P. O. Box. 87317-51167, Islamic Republic of Iran (Iran, Islamic Republic of); Badiei, Alireza [School of Chemistry, College of Science, University of Tehran, Tehran (Iran, Islamic Republic of); Mohandes, Fatemeh [Department of Inorganic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, P. O. Box. 87317-51167, Islamic Republic of Iran (Iran, Islamic Republic of)

    2015-03-15

    Highlights: • For the first time, we have synthesized graphene nanosheets in the presence of pomegranate juice. • Here pomegranate juice was used not only as reductant but also as capping agent. • FT-IR, XRD, SEM, EDS and TEM were used to characterize the samples. • According to TEM image, graphene nanosheet is individually exfoliated after stirring for 24 h. • As shown in the TEM image, graphene monolayer is obtained. - Abstract: For the first time, we have successfully synthesized graphene nanosheets in the presence of pomegranate juice. In this approach, pomegranate juice was used not only as reductant but also as capping agent to form graphene nanosheets. At first, the improved Hummer method to oxidize graphite for the synthesis of graphene oxide (GO) was applied, and then the as-produced graphene oxide was reduced by pomegranate juice to form graphene nanosheets. Fourier transformed infrared (FT-IR), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), high resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM) and raman were used to characterize the samples. The results obtained from the characterization techniques proved high purity of the final products.

  8. Isolation and characterization of three benzylisoquinoline alkaloids from Thalictrum minus L. and their antibacterial activity against bovine mastitis.

    Science.gov (United States)

    Mushtaq, Saleem; Rather, Muzafar Ahmad; Qazi, Parvaiz H; Aga, Mushtaq A; Shah, Aabid Manzoor; Shah, Aiyatullah; Ali, Md Niamat

    2016-12-04

    The roots of Thalictrum minus are traditionally used in the treatment of inflammation and infectious diseases such as bovine mastitis. However, there are no reports available in literature till date regarding the antibacterial studies of T. minus against bovine mastitis. The present study was undertaken to evaluate the antibacterial potential of crude extract of T. minus (root) and some of its isolated constituents against bovine mastitis in order to scientifically validate its traditional use. A total of three alkaloid compounds were isolated from the DCM: MeOH extract of roots of T. minus using silica gel column chromatography. Structural elucidation of the isolated compounds was done by using spectroscopic techniques like mass spectrometry and NMR spectroscopy. Pathogens were isolated from cases of bovine mastitis and identified by using 16S rRNA gene sequencing. The broth micro-dilution method was used to evaluate the antibacterial activities of DCM: MeOH extract and isolated compounds against mastitis pathogens. The three isolated compounds were identified as benzylisoquinoline alkaloids (1) 5'-Hydroxythalidasine, (2) Thalrugosaminine and (3) O-Methylthalicberine. Compounds (2) and (3) are reported for the first time from the roots of T. minus. Five mastitis pathogens viz., Staphylococcus xylosus, Staphylococcus lentus, Staphylococcus equorum, Enterococcus faecalis and Pantoea agglomerans were identified on the basis of sequence analysis of isolates using the nucleotide BLAST algorithm. This study reports for the first time the isolation and molecular characterization of mastitis pathogens from Kashmir valley, India. The DCM: MeOH extract exhibited broad spectrum antibacterial activities that varied between the bacterial species (MIC=250-500µg/ml). 5'-Hydroxythalidasine and Thalrugosaminine showed promising antibacterial activity with MIC values of 64-128µg/ml while Staphylococcus species were found to be the most sensitive strains. The antibacterial

  9. Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer ...

    Indian Academy of Sciences (India)

    ... as anti-bacterial and anti-fungal agents against various microorganisms. The in vitro cytotoxicity tests of the ligand and its copper complex were carried out in two different human tumour cell lines, HCT-116 and MDA – MB - 231. The cytotoxicity studies showed that the complex exhibited higher activity than cisplatin and ...

  10. Synthesis, structure analysis, anti-bacterial and in vitro anti-cancer ...

    Indian Academy of Sciences (India)

    copper complex have been investigated as anti-bacterial and anti-fungal agents against various microorganisms. The in vitro cytotoxicity tests of the ..... integration curves and those obtained from the values of the expected CHN analysis, ... two imino nitrogen atoms to form the equatorial plane, with atoms of the same type in ...

  11. Synthesis and Antibacterial Evaluation of 2-(1,3- Benzodioxol-5 ...

    African Journals Online (AJOL)

    They also act as inhibitors, e.g., histone deacetylase (HDAC), carbonic anhydrase and. HIV protease [1-8]. The chemotherapeutic derivatives of this class are extensively employed as antibacterial and antiviral agents [4,9] since they are structural analogues of p-aminobenzoic acid necessary for the production of folic acid. If.

  12. Synthesis of ZnO nanoparticles and their antibacterial effects | Arefi ...

    African Journals Online (AJOL)

    The zinc oxide nanoparticles with the average particle size of about 30 nm were synthesized by the chemical technique and their properties were studied with the help of scanning electron microscope and X-ray diffraction. The aim of this study was to detect the antibacterial properties of 0.01, 0.5 and 1% nano-ZnO against ...

  13. Synthesis, Characterization and Reactions of (Azidoethynyltrimethylsilane

    Directory of Open Access Journals (Sweden)

    Klaus Banert

    2015-12-01

    Full Text Available Synthesis of azido(trimethylsilylacetylene (6 was performed by treating the iodonium salt 5 with highly soluble hexadecyltributylphosphonium azide (QN3 at −40 °C. Although this product is very unstable, it can nevertheless be trapped by the click reaction with cyclooctyne to give the corresponding 1,2,3-triazole, and also directly characterized by 1H- and 13C-NMR data as well as IR-spectra, which were measured in solution at low temperature and in the gas phase. The thermal or photochemical decay of azide 6 leads to cyano(trimethylsilylcarbene. This is demonstrated not only by quantum chemical calculations, but also by the trapping reactions with the help of isobutene.

  14. Synthesis and characterization of boron nitrides nanotubes

    International Nuclear Information System (INIS)

    Ferreira, T.H.; Sousa, E.M.B.

    2010-01-01

    This paper presents a new synthesis for the production of boron nitride nanotubes (BNNT) from boron powder, ammonium nitrate and hematite tube furnace CVD method. The samples were subjected to some characterization techniques as infrared spectroscopy, thermal analysis, X-ray diffraction and scanning electron microscopy and transmission. By analyzing the results can explain the chemical reactions involved in the process and confirm the formation of BNNT with several layers and about 30 nanometers in diameter. Due to excellent mechanical properties and its chemical and thermal stability this material is promising for various applications. However, BNNT has received much less attention than carbon nanotubes, it is because of great difficulty to synthesize appreciable quantities from the techniques currently known, and this is one of the main reasons this work.(author)

  15. Synthesis and characterization of a cerebral radiotracer

    International Nuclear Information System (INIS)

    Ben hamouda, Salem

    2010-01-01

    The development of nuclear medicine is based on research of new radiopharmaceuticals, in particular, relying on technetium-99m, the most used radioisotope in terms of availability and low cost. A similar study on Rhenium (185/187Re) is essential for monitoring physico-chemical studies due to the high specific activity of technetium-99m. During this work, we have synthesized and labeled with technetium the N-methyl-4-hydroxy piperidinyl ferrocenyl carboxylate. The marking is done by exchange of ligands between the iron group of ferrocene and tricabonyl technetium core. We have succeeded to synthesis the N-methyl-4-hydroxy piperidinyl carboxyl cyclopentadienyl tricarbonyl rhenium (the molecular analogue of the technetium). We characterized it by MS, IR and NMR (1H, 13C) The structure of N-methyl-4-hydroxy piperidinyl carboxyl cyclopentadienyl tricarbonyl technetium is well justified.

  16. Controlled Antibiotic Delivery by Gelatin Nanospheres: Optimization, Characterization and Antibacterial Evaluation

    Directory of Open Access Journals (Sweden)

    Shahrzad Fathollahipour

    2016-10-01

    Full Text Available The present work focuses on preparation and characterization of erythromycin loaded gelatin nanoparticles through nanoprecipitation method. The procedure consists of the addition of the aqueous gelatin solution to the non-solvent phase containing Lutherol F127. Three different measures of cross-linker and polymer concentration were also examined, and the optimum concentration was found. The morphology of gelatin nanoparticles was characterized by field emission scanning electron microscope. It was shown that the optimal morphology can be achieved at the concentration of 1.25 wt % of gelatin in aqueous phase by addition of 20 mL of glutaraldehyde 5%, as the crosslinking agent. Nanoparticle wet size determination was carried out using a dynamic light scattering system and found to be approximately 100 nm. Furthermore, Erythromycin release studies proved the suitability of these particles as a drug delivery system, at least in the studied 72 hours interval. As suggested by related measurements, these nanoparticles are good candidates for antibacterial agent release in any possible related application.

  17. Spanish Broom (Spartium junceum L.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: Preparation, characterization and antibacterial activity.

    Science.gov (United States)

    Cerchiara, Teresa; Abruzzo, Angela; Ñahui Palomino, Rogers Alberto; Vitali, Beatrice; De Rose, Renata; Chidichimo, Giuseppe; Ceseracciu, Luca; Athanassiou, Athanassia; Saladini, Bruno; Dalena, Francesco; Bigucci, Federica; Luppi, Barbara

    2017-03-01

    In this work, we propose as new wound dressing, the Spanish Broom fibers impregnated with vancomycin (VM) loaded chitosan nanoparticles. Spanish Broom fibers were extracted by patented method DiCoDe and the morphological, physical and mechanical properties were investigated. Chitosan nanoparticles were prepared by ionic gelation using different weight ratios between chitosan (CH) and tripolyphosphate (TPP). Nanoparticles were characterized in terms of size, zeta potential, yield, encapsulation efficiency, stability and drug release. Finally, the antibacterial activity against Staphylococcus aureus as well as in vitro cytotoxicity on HaCaT cells were evaluated. The best formulation CH/TPP 4:1 was selected based on the encapsulation efficiency and yield. Spanish Broom fibers impregnated with loaded nanoparticles showed an increased antibacterial activity against S. aureus compared to the same fibers containing VM without nanoparticles. Moreover, these fibers were not toxic to HaCaT keratinocytes cells. In conclusion, Spanish Broom fibers impregnated with VM loaded CH/TPP nanoparticles would appear to be a promising candidate for wound dressing application. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. One-step flame synthesis of silver nanoparticles for roll-to-roll production of antibacterial paper

    Science.gov (United States)

    Brobbey, Kofi J.; Haapanen, Janne; Gunell, Marianne; Mäkelä, Jyrki M.; Eerola, Erkki; Toivakka, Martti; Saarinen, Jarkko J.

    2017-10-01

    Nanoparticles are used in several applications due to the unique properties they possess compared to bulk materials. Production techniques have continuously evolved over the years. Recently, there has been emphasis on environmentally friendly manufacturing processes. Substrate properties often limit the possible production techniques and, for example; until recently, it has been difficult to incorporate nanoparticles into paper. Chemical reduction of a precursor in the presence of paper changes the bulk properties of paper, which may limit intended end-use. In this study, we present a novel technique for incorporating silver nanoparticles into paper surface using a flame pyrolysis procedure known as Liquid Flame Spray. Papers precoated with mineral pigments and plastic are used as substrates. Silver nanoparticles were analyzed using SEM and XPS measurements. Results show a homogeneous monolayer of silver nanoparticles on the surface of paper, which demonstrated antibacterial properties against E. coli. Paper precoated with plastic showed more nanoparticles on the surface compared to pigment coated paper samples except for polyethylene-precoated paper. The results demonstrate a dry synthesis approach for depositing silver nanoparticles directly onto paper surface in a process which produces no effluents. The production technique used herein is up scalable for industrial production of antibacterial paper.

  19. Synthesis, structural and antibacterial study of new silver complex with 3-acetyl-2H chromene-2-one

    Directory of Open Access Journals (Sweden)

    Z. Ali

    2017-01-01

    Full Text Available A new silver complex [Ag(C11H8O32]NO3 was synthesized by the reaction of silver nitrateand coumarin based ligand (3-acetyl-2H-chromene-2-one through solution method. The product was characterized using different analytical techniques like melting point, Infrared spectroscopy, Raman spectroscopy, powder X-ray diffraction, thermogravimetric analysis, scanning electron microscopy, atomic absorption spectroscopy and mass spectrometry. An antibacterial study of the complex was also studied for its possible use in medical treatment.

  20. Synthesis and Antibacterial Activity of New Spiro[thiadiazoline-(pyrazolo[3,4-d]pyrimidine] Derivatives

    Directory of Open Access Journals (Sweden)

    Mohammed El Fal

    2015-01-01

    Full Text Available New heterocyclic compounds spiroderivatives of allopurinol of biological interest were prepared from allopurinol via thionation and 1,3-dipolar cycloaddition and were produced in high to excellent yields. These compounds were characterized on the basis of spectral and spectroscopic data (1H NMR, 13C, IR, and MS. The antibacterial activity of the synthesized products was studied using bacterial strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. Compounds having an ethyl group showed the best activity with MIC value of 31.25 µg/mL against Staphylococcus aureus and Streptococcus fasciens.

  1. 4-Hydroxy-2-pyridones: Discovery and evaluation of a novel class of antibacterial agents targeting DNA synthesis.

    Science.gov (United States)

    Arnold, Michael A; Gerasyuto, Aleksey I; Wang, Jiashi; Du, Wu; Gorske, Yi Jin Kim; Arasu, Tamil; Baird, John; Almstead, Neil G; Narasimhan, Jana; Peddi, Srinivasa; Ginzburg, Olya; Lue, Stanley W; Hedrick, Jean; Sheedy, Josephine; Lagaud, Guy; Branstrom, Arthur A; Weetall, Marla; Prasad, J V N Vara; Karp, Gary M

    2017-11-15

    The continued emergence of bacteria resistant to current standard of care antibiotics presents a rapidly growing threat to public health. New chemical entities (NCEs) to treat these serious infections are desperately needed. Herein we report the discovery, synthesis, SAR and in vivo efficacy of a novel series of 4-hydroxy-2-pyridones exhibiting activity against Gram-negative pathogens. Compound 1c, derived from the N-debenzylation of 1b, preferentially inhibits bacterial DNA synthesis as determined by standard macromolecular synthesis assays. The structural features of the 4-hydroxy-2-pyridone scaffold required for antibacterial activity were explored and compound 6q, identified through further optimization of the series, had an MIC 90 value of 8 μg/mL against a panel of highly resistant strains of E. coli. In a murine septicemia model, compound 6q exhibited a PD 50 of 8 mg/kg in mice infected with a lethal dose of E. coli. This novel series of 4-hydroxy-2-pyridones serves as an excellent starting point for the identification of NCEs treating Gram-negative infections. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Synthesis and antibacterial activity of some new 3-hydroxy-2-naphthoic acid hydrazide derivatives for dyeing polyester fabrics

    Directory of Open Access Journals (Sweden)

    Abd El-Galil M. Khalil

    2016-03-01

    Full Text Available Dibenzobarallene was used as a key intermediate for the synthesis of 2-(3-hydroxy-2-naphthoyl-3,4,4a,5,10,10a-hexahydro-1H-5,10-benzenobenzo[g]phthalazine-1,4-dione (2. The previous compound was coupled with the appropriate diazonium chloride to give the corresponding 4-arylazo-2-naphthol derivatives 3a–l. Also, nitration, nitrosation and bromination of compound 2 afforded the corresponding nitro, nitroso and bromo derivatives 4–6, respectively. The synthesized compounds were established and evaluated as antibacterial agents. The results showed clearly that compounds 2, 3a, 3c, 3d, 3g, 3i, 4, 5 and 6 exhibited interesting high activities compared with reference drugs. Also, these compounds were applied to polyester as disperse dyes in which their color measurement and fastness properties were evaluated.

  3. Synthesis, characterization and evaluation of bioactivity and ...

    Indian Academy of Sciences (India)

    Bioactive glass; zinc; bioactivity; antibacterial activity; tissue engineering. ... Biomaterials Group, Faculty of Biomedical Engineering (Centre of Excellence), Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran; Department of Biotechnology and Cellular and Molecular Research Centre, Faculty of Applied ...

  4. Synthesis, characterization and antimicrobial studies of transition ...

    African Journals Online (AJOL)

    A series of new biologically active complexes of Zn(II), Cu(II), Co(II) and Ni(II) with imidazole derivative have been synthesized. The synthesized chelating agent and metal(II) complexes were screened for antibacterial activities against four pathogenic species of bacteria namely; Eschereschi coli, Pseudomonas aeruginosa, ...

  5. Synthesis of polystyrene@(silver-polypyrrole) core/shell nanocomposite microspheres and study on their antibacterial activities

    Science.gov (United States)

    Guo, Longhai; Ren, Shanshan; Qiu, Teng; Wang, Leilei; Zhang, Jiangru; He, Lifan; Li, Xiaoyu

    2015-01-01

    We reported the synthesis of polystyrene@(silver-polypyrrole) (PS@(Ag-PPy)) nanocomposite microspheres with the well-defined core/shell structure, in which the functionalized PS microspheres by the sulfonic acid groups were employed as template. The diameter of the synthesized PS microsphere template and AgNP was 1.26 μm and 50 nm, respectively. In order to well control the redox reaction between Ag+ and Py monomer and to avoid the accumulation of these AgNPs during synthesis process, the complexation of triethanolamine (TEA) and silver ion ([Ag(TEA)2]+) was employed as the oxidant, so that the generation rate of AgNPs was in turn decreased. Moreover, compared with the redox reaction between AgNO3 and Py, the introduction of [Ag(TEA)2]+ ions resulted in the improved coverage and distribution of AgNPs around the surface of PS microspheres. Meanwhile, the loading amount of Ag-PPy nanocomposites on the final microspheres was adjustable. The increasing concentrations of Py monomer and [Ag(TEA)2]+ ions resulted in the increase of Ag-PPy nanocomposite loading. The results of antibacterial experiment suggested that the synthesized PS@(Ag-PPy) composite microspheres showed the prominent antibacterial properties against both the Gram-negative bacteria of Escherichia coli and the Gram-positive bacteria of Staphylococcus aureus. For the bacteria with concentration at 1 × 105 - 9×105 cfu/mL, the microspheres can kill the bacteria above 3-log reduction with the concentration of PS@(Ag-PPy) composite microspheres at 50 μg/mL, in which the weight fraction of Py in the composite microspheres was above 10 wt%. When the weight fraction of Py in the composite microspheres was at 5 wt%, the 2-log reduction of in bacterial viability could also be obtained.

  6. Synthesis and characterization of dental composites

    Science.gov (United States)

    Djustiana, Nina; Greviana, Nadia; Faza, Yanwar; Sunarso

    2018-02-01

    During the last few decades, the increasing demands in esthetic dentistry have led to the development of dental composites material that provide similar appearance to the natural teeth. Recently, esthetic trend was an issue which increase the demand for teeth restorations that is similar with the origin. The esthetics of dental composite are more superior compared to amalgam, since its color look similar with natural teeth. Various dental composites have been developed using many type of fillers such as amorphous silica, quartz), borosilicate, Li-Sr-Ba-Al glass and oxide: zirconia and alumina. Researchers in Faculty of Dentistry University of Padjadjaran have prepared dental composites using zirconia-alumina-silica (ZAS) system as the filler. The aim is to improve the mechanical properties and the esthetic of the dental composites. The ZAS was obtained from chemical grade purity chemicals and Indonesia's natural sand as precursors its characterization were also presented. This novel method covers the procedure to synthesis and characterize dental composites in Padjadjaran University and some review about dental composites in global research.

  7. Characterization of a human peptide deformylase: implications for antibacterial drug design.

    Science.gov (United States)

    Nguyen, Kiet T; Hu, Xubo; Colton, Craig; Chakrabarti, Ratna; Zhu, Michael X; Pei, Dehua

    2003-08-26

    Ribosomal protein synthesis in eubacteria and eukaryotic organelles initiates with an N-formylmethionyl-tRNA(i), resulting in N-terminal formylation of all nascent polypeptides. Peptide deformylase (PDF) catalyzes the subsequent removal of the N-terminal formyl group from the majority of bacterial proteins. Deformylation was for a long time thought to be a feature unique to the prokaryotes, making PDF an attractive target for designing novel antibiotics. However, recent genomic sequencing has revealed PDF-like sequences in many eukaryotes, including man. In this work, the cDNA encoding Homo sapiens PDF (HsPDF) has been cloned and a truncated form that lacks the N-terminal 58-amino-acid targeting sequence was overexpressed in Escherichia coli. The recombinant, Co(2+)-substituted protein is catalytically active in deformylating N-formylated peptides, shares many of the properties of bacterial PDF, and is strongly inhibited by specific PDF inhibitors. Expression of HsPDF fused to the enhanced green fluorescence protein in human embryonic kidney cells revealed its location in the mitochondrion. However, HsPDF is much less active than its bacterial counterpart, providing a possible explanation for the apparent lack of deformylation in the mammalian mitochondria. The lower catalytic activity is at least partially due to mutation of a highly conserved residue (Leu-91 in E. coli PDF) in mammalian PDF. PDF inhibitors had no detectable effect on two different human cell lines. These results suggest that HsPDF is likely an evolutional remnant without any functional role in protein formylation/deformylation and validates PDF as an excellent target for antibacterial drug design.

  8. Synthesis, Structural and Antibacterial Studies of New Dithiocarbamate Complexes of Sb (III) And Bi (III)

    International Nuclear Information System (INIS)

    Nur Amirah Jamaluddin; Ibrahim Baba; Nazlina Ibrahim

    2014-01-01

    Six new dithiocarbamate complexes from three different amines, for example N-ethyl ethanol-, N-butylethyl- and N-benzylmethylamine were successfully prepared using in situ method. All complexes were characterized by elemental analysis, IR, UV-Vis, 13 C and 1 H NMR. Elemental analysis data (C, H, N and S) showed an agreement with the general formula of MCl(S 2 CNR ' R '' ) 2 , (M = Sb (III), Bi (III); R ' = ethyl, butyl and benzyl; R '' = ethanol, ethyl and methyl). The complexes had been characterized by infrared spectroscopy that showed a thioureido bands, v(C-N) in the region of 1427 - 1490 cm -1 followed by v(C-S) bands that can be seen in the region of 935 - 1060 cm -1 and v(M-S) bands existed in the region of 350 - 392 cm -1 . Maximum wavelength absorption for ultraviolet-visible spectroscopy for N-C-S group was at 254 nm which indicates π → π * transition. Data from 13 C NMR showed a signal in the region of 198.39 - 199.44 ppm that corresponded to the NCS 2 group. The crystal structure of bis(N,N ' utylethyldithiocarbamato)chloride antimony (III), SbCl (S 2 CN(C 4 H 9 )(C 2 H 5 )) 2 demonstrated a five-coordination geometry, triclinic system, space group P1 with a = 10.0141(8) Armstrong, b = 10.1394(7) Am strong, c = 11.8665(9) Armstrong, α = 67.960(2) degree, β = 87.616(2) degree and γ = 80.172(2) degree. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were done using five dithiocarbamate complexes which are Bi Cl[S 2 CN(C 2 H 5 )(C 2 H 4 OH)) 2 , SbCl[S 2 CN(C 2 H 5 )(C 2 H 4 OH)) 2 , Bi Cl(S 2 CN(C 4 H 9 )(C 2 H 5 )) 2 , SbCl(S 2 CN(C 4 H 9 )(C 2 H 5 )) 2 , Bi Cl(S 2 CN(C 7 H 7 )(CH 3 )) 2 towards S. aureus, S. epidermidis, E. aerogenes and E. coli. It was found that all four complexes were active against S. aureus except SbCl(S 2 CN(C 2 H 5 )(C 2 H 4 OH)) 2 while Bi Cl(S 2 CN(C 7 H 7 )(CH 3 )) 2 and Bi Cl(S 2 CN(CH 3 )(C 6 H 11 )) 2 complexes were active against S. epidermidis with MIC value of 5.0 mg/ m

  9. [Characterization and antibacterial effect of Ag-nHA-nTiO2/polyamide 66 nanocomposite membrane on oral bacteria].

    Science.gov (United States)

    Bai, Shi; Mo, An-chun; Xian, Su-qin; Zuo, Yi; Li, Yu-bao; Xu, Wei

    2008-08-01

    Undried silver-hydroxyapatite-titania (Ag-nHA-nTiO2) nanoparticles slurry was used to make membrane with polyamide 66 (PA66) by co-polymerization method. The purpose of this study is to test the physical and chemical characteristics and antibacterial ability. The morphology, chemical components and structures of the membrane were characterized by atomic absorption spectrometer (AAS), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy-dispersive X-ray analysis (EDX). Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum) and Streptococcus mutans (S. mutans) were utilized to test the antibacterial effect. XRD results demonstrated that the membrane have characteristic diffraction peaks of pure hydroxyapatite (HA). A homogeneous distribution of the Ca, P, Ti and Ag element in the membrane was confirmed by EDX. Both surface and section showed porous structure which was confirmed by SEM and the average hole size was 20-30 microm. The bacteria assay reflected to the antibacterial effect, 50.10% of S. aureus and 56.31% of E. coli were killed. However, 91.84% of P. gingivalis, 90.64% of F. nucleatum and 90.49% of S. mutans were killed and pictures of SEM showed obviously fewer cells on the surface. The nanocomposite membrane could be one of the bioactive materials with antibacterial properties for oral guided bone regeneration technique.

  10. Preparation, characterization, and antibacterial activity studies of silver-loaded poly(styrene-co-acrylic acid) nanocomposites.

    Science.gov (United States)

    Song, Cunfeng; Chang, Ying; Cheng, Ling; Xu, Yiting; Chen, Xiaoling; Zhang, Long; Zhong, Lina; Dai, Lizong

    2014-03-01

    A simple method for preparing a new type of stable antibacterial agent was presented. Monodisperse poly(styrene-co-acrylic acid) (PSA) nanospheres, serving as matrices, were synthesized via soap-free emulsion polymerization. Field-emission scanning electron microscopy micrographs indicated that PSA nanospheres have interesting surface microstructures and well-controlled particle size distributions. Silver-loaded poly(styrene-co-acrylic acid) (PSA/Ag-NPs) nanocomposites were prepared in situ through interfacial reduction of silver nitrate with sodium borohydride, and further characterized by transmission electron microscopy and X-ray diffraction. Their effects on antibacterial activity including inhibition zone, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and bactericidal kinetics were evaluated. In the tests, PSA/Ag-NPs nanocomposites showed excellent antibacterial activity against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli. These nanocomposites are considered to have potential application in antibacterial coatings on biomedical devices to reduce nosocomial infection rates. Copyright © 2013 Elsevier B.V. All rights reserved.

  11. Tragacanth gum/nano silver hydrogel on cotton fabric: In-situ synthesis and antibacterial properties.

    Science.gov (United States)

    Montazer, M; Keshvari, A; Kahali, P

    2016-12-10

    This paper is mainly focused on introducing cotton fabric with hydrogel and antimicrobial properties using Tragacanth gum as a natural polymer with hydrogel properties, silver nitrate as silver precursor, citric acid as a cross-linking agent and sodium hypophosphite as catalyst. The water absorption behavior of the treated fabrics was investigated with moisture regain, water retention, drying time of wetted fabric at room condition and vertical wicking tests. Antibacterial properties of the samples were evaluated against Escherichia coli and Staphylococcous aureus. The SEM pictures confirmed formation of nano silver and hydrogel layer on the fabric surface and XRD performed the crystal and particle size of the nano silver. The chemical structure of the fabric samples was identified with FTIR spectra. The central composite design (CCD) was used for statistical modelling, evaluated effective parameters and created optimum conditions. The treated cotton fabrics showed good water absorption properties along with reasonable antibacterial effectiveness. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Synthesis and antibacterial studies of 2-aryl-3-alkanamido-4H-thiazolidin-4-one derivatives

    OpenAIRE

    Sandeep Jain; Ashwani Kumar; Mahesh Kumar; Neelam Jain

    2016-01-01

    A series of 2-aryl-3-alkanamido-4H-thiazolidin-4-ones were synthesized from long chain fatty acid hydrazides and studied for their in vitro antibacterial activity. Long chain fatty acid hydrazides 1 on reaction with different aromatic aldehydes in the presence of catalytic amount of glacial acetic acid yielded the corresponding aryl hydrazones 2 which on further reaction with thioglycolic acid in the presence of anhydrous zinc chloride furnished the title compounds 3. These compounds were cha...

  13. Synthesis and antibacterial evaluation of calcinated Ag-doped nano-hydroxyapatite with dispersibility.

    Science.gov (United States)

    Furuzono, Tsutomu; Motaharul, Mazumder; Kogai, Yasumichi; Azuma, Yoshinao; Sawa, Yoshiki

    2015-05-01

    Dispersible hydroxyapatite (HAp) nanoparticles are very useful for applying a monolayer to implantable medical devices using the nano-coating technique. To improve tolerance to infection on implanted medical devices, silver-doped HAp (Ag-HAp) nanoparticles with dispersiblity and crystallinity were synthesized, avoiding calcination-induced sintering, and evaluated for antibacterial activity. The Ca10-xAgx(PO4)6(OH)2 with x = 0 and 0.2 were prepared by wet chemical processing at 100°C. Before calcination at 700°C for 2 h, two kinds of anti-sintering agents, namely a Ca(NO3)2 (Ca salt) and a polyacrylic acid/Ca salt mixture (PAA-Ca), were used. Escherichia coli was used to evaluate the antibacterial activity of the nanopowder. When PAA-Ca was used as an anti-sintering agent in calcination to prepare the dispersible nanoparticles, strong metallic Ag peaks were observed at 38.1° and 44.3° (2θ) in the X-ray diffraction (XRD) profile. However, the Ag peak was barely observed when Ca salt was used alone as the anti-sintering agent. Thus, using Ca salt alone was more effective for preparation of dispersible Ag-HAp than PAA-Ca. The particle average size of Ag-HAp with 0.5 mol% of Ag content was found to be 325 ± 70 nm when the formation of large particleaggregations was prevented, as determined by dynamic light scattering instrument. The antibacterial activity of the Ag-HAp nanoparticles possessing 0.5 mol% against E. coli was greater than 90.0%. Dispersible and crystalline nano Ag-HAp can be obtained by using Ca salt alone as an anti-sintering agent. The nanoparticles showed antibacterial activity.

  14. Synthesis and Antibacterial Evaluation of Some Novel Imidazole and Benzimidazole Sulfonamides

    Directory of Open Access Journals (Sweden)

    Aidil Abdul Hamid

    2013-09-01

    Full Text Available Several new substituted sulfonamide compounds were synthesized and their structures were confirmed by 1H-NMR, 13C-NMR, FT-IR, and mass spectroscopy. The antibacterial activities of the synthesized compounds were screened against standard strains of six Gram positive and four Gram negative bacteria using the microbroth dilution assay. Most of the compounds studied showed promising activities against both types of bacteria.

  15. Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity.

    Science.gov (United States)

    Balashanmugam, Pannerselvam; Kalaichelvan, Pudupalayam Thangavelu

    2015-01-01

    The present study reports the green synthesis of silver nanoparticles (AgNPs) from silver precursor using a plant biomaterial, Cassia roxburghii DC., aqueous extract. The AgNPs were synthesized from the shade-dried leaf extract and assessed for their stability; they elucidated characteristics under UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy dispersive X-ray spectroscopy. The synthesized AgNPs exhibited a maximum absorption at 430 nm, and the X-ray diffraction patterns showed that they were crystal in nature. Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping material of plant extract. The HR-TEM analysis revealed that they are spherical ranging from 10 nm to 30 nm. The spot EDAX analysis showed the presence of silver atoms. In addition, AgNPs were evaluated for their antibacterial activity against six different pathogenic bacteria: three Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus, and three Gram-negative bacteria, Pseudomonas aeruginosa, Escherichia coli, and Enterobacter aerogenes. They were highly sensitive to AgNPs, whereas less sensitive to AgNO3. Furthermore, the green synthesized AgNPs were immobilized on cotton fabrics and screened for antibacterial activity. The immobilized AgNPs on cotton cloth showed high antibacterial activity. Therefore, they could be a feasible alternative source in treating wounds or may help in replacing pharmaceutical band-aids.

  16. Production of putrescine-capped stable silver nanoparticle: its characterization and antibacterial activity against multidrug-resistant bacterial strains

    Science.gov (United States)

    Saha, Saswati; Gupta, Bhaskar; Gupta, Kamala; Chaudhuri, Mahua Ghosh

    2016-11-01

    Integration of biology with nanotechnology is now becoming attention-grabbing area of research. The antimicrobial potency of silver has been eminent from antiquity. Due to the recent desire for the enhancement of antibacterial efficacy of silver, various synthesis methods of silver in their nano dimensions are being practiced using a range of capping material. The present work highlights a facile biomimetic approach for production of silver nanoparticle being capped and stabilized by putrescine, possessing a diameter of 10-25 ± 1.5 nm. The synthesized nanoparticles have been analyzed spectrally and analytically. Morphological studies are carried out by high-resolution transmission electron microscopy and crystallinity by selected area electron diffraction patterns. Moreover, the elemental composition of the capped nanoparticles was confirmed by energy-dispersive X-ray spectroscopy analysis. A comparative study (zone of inhibition and minimum inhibitory concentration) regarding the interactions and antibacterial potentiality of the capped silver nanoparticles with respect to the bare ones reveal the efficiency of the capped one over the bare one. The bacterial kinetic study was executed to monitor the interference of nanoparticles with bacterial growth rate. The results also highlight the efficacy of putrescine-capped silver nanoparticles as effective growth inhibitors against multi-drug resistant human pathogenic bacterial strains, which may, thus, potentially be applicable as an effective antibacterial control system to fight diseases.

  17. Synthesis and characterization of nickel complexes derived from Schiff-Bases

    Directory of Open Access Journals (Sweden)

    Mirian Y. Matsumoto

    2012-06-01

    Full Text Available Aromatic Schiff-Bases constitute a large class of organic compounds containing the imine group (C=N [1-3]. 2-hydroxybenzaldehyde (salicylaldehyde which has been frequently used for the synthesis of aromatic Schiff-Bases, it has many biological applications, such as analgesics, anti-inflammatory, antibacterial, antiviral and bactericidal activities [4-6]. From above, this project proposed the synthesis of three Schiff bases derived from salicylaldehyde prepared by condensation between salicylaldehyde with different p-substituted anilines (H, NO2 and MeO. Schiff-Bases synthesized were used as starting compound for the synthesis of nickel complexes. The synthesis of nickel complexes were carried out by adding of alcoholic solution of ligands (2 eq. in aqueous solution of NiCl2.6 H2O (1 eq. contain KOH (1eq. 3 hours. After thisMatsumoto et al. time, the reaction was kept to r.t. to obtain a precipitates. The precipitates were filtered under vacuum and they were characterized by GC-MS, FT-IR and UV-Vis

  18. Synthesis and characterization of fluorine compounds

    International Nuclear Information System (INIS)

    Martinez Carrillo, M.

    1991-01-01

    The ( 18 F) D-glucose, 2-deoxy fluorine ( 18 FDG) is a radio pharmaceutic that is used in nuclear medicine it is utilized mainly in the glucose metabolism. It allows recently to observe the tumors accumulation and growing. The obtention of this radio pharmaceutic can realize by a nucleophilic or electrophilic process through the use of different fluorinated agents obtained as intermediates for introducing the 18 F radionuclide in a final step of synthesis. The first methods already has been studied in the National Institute of Nuclear Research. The second one which is based this work and it was realized through the reaction of acetyl hypo fluorite (CH 3 COOF) with tri acetyl glucal (TAG) in turn they require the obtention of several fluorated compounds that they serve as intermediates for their obtention so that objective of this work was to find the adequate technique for the obtention of anhydride hydrofluoric acid (HF), KF.2 HF and elemental fluorine so as the design and construction of the systems and equipment used for carry out each one of the reactions. Moreover it was designed the system that will be used for the obtention of acetyl hypo fluoride and the synthesis of composite tetraacetilide 3,4,6 tri-D-glucopyranosil fluoride (TAG-F) for that finally by hydrolysis it was obtained the 2-deoxy fluoride-D-glucose (TAG) in inactive. In this system were realized several preliminary tests. The results are showed in the content of this work also the techniques for compounds characterization were given. (Author)

  19. Synthesis and Characterization of Nano Scale YBCO

    International Nuclear Information System (INIS)

    Sukirman, E.; Wisnu AA; Yustinus P; Sahidin W, D.; Rina M, Th.

    2009-01-01

    Synthesis and characterization of the nano scale YBCO superconductor have been performed. The nano scale superconductor was synthesized from YBCO system (YBa 2 Cu 3 O 7-X ). Raw materials, namely Y 2 O 3 , BaCO 3 , and Cu°, were balanced and mixed with ethanol using magnetic steering as a churn in a beaker glass. Then, the precursor was calcined at T k = 900°C for 5 hours and repeated it until three times. The resulting precursor was ground by using High Energy Milling (HEM) for t = 0, 30, 50, 70, and 90 hour and hereinafter precursors are successively referred as YKM-00, YKM-30, YKM-50, YKM-70, and YKM-90. The resulting powders phase were characterized by means of x-ray diffraction technique using the Rietveld analysis method. Precursor of YKM-90 was pressed into pellets, and then sintered at various temperatures and periods. The sample phase was then characterized by using the Rietveld analysis method based on the x-ray diffraction data. The crystallites size were calculated using Scherrer formula. Results of analysis indicate that by minimizing crystallites size, period of sinter can be shortened from 10 to 1 hour, resulting crystallite size of D = 925 Å, critical current density of J c = 4 A / cm 2 , and can be grown of about 15 weight % of 211-phase in a matrix of 123-phase. The decrease of crystallite size will generate a change in physical properties dramatically, if the crystallite size of the material, D is smaller or equal to the coherence length of 10 Å. (author)

  20. Synthesis and bioactivities study of new antibacterial peptide mimics: The dialkyl cationic amphiphiles.

    Science.gov (United States)

    Zhang, En; Bai, Peng-Yan; Cui, De-Yun; Chu, Wen-Chao; Hua, Yong-Gang; Liu, Qin; Yin, Hai-Yang; Zhang, Yong-Jie; Qin, Shangshang; Liu, Hong-Min

    2018-01-01

    The emergence of infectious diseases caused by pathogenic bacteria is widespread. Therefore, it is urgently required to enhance the development of novel antimicrobial agents with high antibacterial activity and low cytotoxicity. A series of novel dialkyl cationic amphiphiles bearing two identical length lipophilic alkyl chains and one non-peptidic amide bond were synthesized and tested for antimicrobial activities against both Gram-positive and Gram-negative bacteria. Particular compounds synthesized showed excellent antibacterial activity toward drug-sensitive bacteria such as S. aureus, E. faecalis, E. coli and S. enterica, and clinical isolates of drug-resistant species such as methicillin-resistant S. aureus (MRSA), KPC-producing and NDM-1-producing carbapenem-resistant Enterobacteriaceae (CRE). For example, the MIC values of the best compound 4g ranged from 0.5 to 2 μg/mL against all these strains. Moreover, these small molecules acted rapidly as bactericidal agents, and functioned primarily by permeabilization and depolarization of bacterial membranes. Importantly, these compounds were difficult to induce bacterial resistance and can potentially combat drug-resistant bacteria. Thus, these compounds can be developed into a new class of antibacterial peptide mimics against Gram-positive and Gram-negative bacteria, including drug-resistant bacterial strains. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  1. Synthesis of water-based cationic polyurethane for antibacterial and gene delivery applications.

    Science.gov (United States)

    Wu, Geng-Hsi; Hsu, Shan-Hui

    2016-10-01

    Cationic polymers are often used as antimicrobial materials and transfection reagents. Water-based process could reduce environmental pollution and prevent the risk of solvent residue in the final product. In this study, waterborne biodegradable cationic polyurethane (WCPU) was synthesized by reacting polycaprolactone (PCL diol), isophorone diisocyanate (IPDI), and N-methyldiethanolamine (N-MDEA) under 75°C. An aqueous dispersion of WCPU nanoparticles (NPs) could be acquired by vigorous stirring under acidic condition. The particles in the dispersion had an average size of ∼80nm and a zeta potential of ∼60mV. When cast into films, the contact angle of the film was ∼67° and the zeta potential was ∼16mV. WCPU NPs demonstrated excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) (100% inhibition with a contact time of 3h). Meanwhile, the antibacterial ratio of WCPU films to E. coli and S. aureus reached 100% after 24h of contact. Moreover, WCPU NPs could be used as a transfection reagent without significant toxicity for concentrations less than 1000μg/mL and showed the ability to condensate plasmid DNA. The transfection efficiency for HEK293T cells and hBMSCs was ∼60% and ∼30% at 48h, respectively, after the transfection. Therefore, the WCPU synthesized in this study has potential antibacterial and gene delivery applications. Copyright © 2016 Elsevier B.V. All rights reserved.

  2. A new large - Scale synthesis of magnesium oxide nanowires: Structural and antibacterial properties

    Science.gov (United States)

    Al-Hazmi, Faten; Alnowaiser, Fowzia; Al-Ghamdi, A. A.; Al-Ghamdi, Attieh A.; Aly, M. M.; Al-Tuwirqi, Reem M.; El-Tantawy, Farid

    2012-08-01

    Large-scale one-dimensional magnesium oxide (MgO) nanowires with diameters of 6 nm and lengths of 10 μm have been successfully synthesized by a new facile and simple reaction. This production was performed via a microwave hydrothermal approach at low temperature growth of 180 °C for 30 min. The structure of as synthesized MgO nanowires were investigated by means of X-ray diffraction (X-ray), Fourier Transformation Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FE-SEM), Transmission Electron Microscopy (TEM), Selected Area Electron Diffraction (SAED) and Energy Dispersive X-ray (EDS). The antibacterial behavior of MgO nanowires concentration in solid media against Gram negative and Gram positive for different bacteria has been tested in details. The results show that the MgO nanowires have bacteriostatic activity against Escherichia coli and Bacillus sp. The antibacterial activity increases with increasing MgO nanowires concentration. Furthermore, the presence of one-dimensional MgO nanowires has high antibacterial efficacy and damages the membrane wall of bacteria. Finally, this study offered the prospect of developing ultrafine nanoscale devices utilizing MgO nanowires and implementing their useful potential in biological control.

  3. Isolation and Characterization of Antibacterial Compound from a Mangrove-Endophytic Fungus, Penicillium chrysogenum MTCC 5108.

    Science.gov (United States)

    Devi, Prabha; Rodrigues, Cheryl; Naik, C G; D'Souza, L

    2012-12-01

    Microorganisms, especially endophytic fungi that reside in the tissue of living mangrove plants, seem to play a major role in meeting the general demand for new biologically active substances. During the course of screening for biologically active secondary metabolites from marine microorganisms, an antibiotic compound containing an indole and a diketopiperazine moiety was isolated from the culture medium of Penicillium chrysogenum, (MTCC 5108), an endophytic fungus on the mangrove plant Porteresia coarctata (Roxb.). The cell free culture medium of P. chrysogenum showed significant activity against Vibrio cholerae, (MCM B-322), a pathogen causing cholera in humans. Bioassay guided chemical characterization of the crude extract led to the isolation of a secondary metabolite possessing a molecular formula C19H21O2N3. Its antibacterial activity was comparable with standard antibiotic, streptomycin. This compound (1) was found to be (3,1'-didehydro-3[2″(3'″,3'″-dimethyl-prop-2-enyl)-3″-indolylmethylene]-6-methyl pipera-zine-2,5-dione) on the basis of mass spectrometry, infrared spectroscopy and one and two-dimensional nuclear magnetic resonance analysis.

  4. Characterization in vitro studies and antibacterial properties on a sol-gel derived silver incorporated bioglass

    Science.gov (United States)

    Bouhazma, S.; Chajri, S.; Khaldi, M.; Sadiki, M.; Barkai, H.; Elabed, S.; Ibnsouda Koraichi, S.; El Bali, B.; Lachkar, M.

    2017-03-01

    The SiO2-CaO, SiO2-CaO-P2O5 and SiO2-CaO-P2O5-Ag2O glass systems were synthesized by the sol-gel technique and characterized with different techniques such as X-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), and Environmental Scanning Electron Microscopy (ESEM). In vitro bioactivity tests were performed in Simulated Body Fluid (SBF). The antibacterial action of 65S5Ag (65%SiO2 + 24%CaO + 6%P2O5 + 5% Ag2O) is attributed exclusively to the leaching of Ag+ ions from the glass matrix. The activity of SiO2-CaO-P2O5-Ag2O was compared with that of its binary and ternary counterpart glass system. The concentrations of Ag-bioglass, in the range of 0.05 mg/mL of culture medium, were found to inhibit the growth of these bacteria.

  5. Extracellular biosynthesis of silver nanoparticles using a novel and non-pathogenic fungus, Neurospora intermedia: controlled synthesis and antibacterial activity.

    Science.gov (United States)

    Hamedi, Sepideh; Shojaosadati, Seyed Abbas; Shokrollahzadeh, Soheila; Hashemi-Najafabadi, Sameereh

    2014-02-01

    In the present study, the biosynthesis of silver nanoparticles (AgNPs) using Neurospora intermedia, as a new non-pathogenic fungus was investigated. For determination of biomass harvesting time, the effect of fungal incubation period on nanoparticle formation was investigated using UV-visible spectroscopy. Then, AgNPs were synthesized using both culture supernatant and cell-free filtrate of the fungus. Two different volume ratios (1:100 and 1:1) of the culture supernatant to the silver nitrate were employed for AgNP synthesis. In addition, cell-free filtrate and silver nitrate were mixed in presence and absence of light. Smallest average size and highest productivity were obtained when using equal volumes of the culture supernatant and silver nitrate solution as confirmed by UV-visible spectra of colloidal AgNPs. Comparing the UV-visible spectra revealed that using cell-free filtrate for AgNP synthesis resulted in the formation of particles with higher stability and monodispersity than using culture supernatant. The absence of light in cell-free filtrate mediated synthesis led to the formation of nanoparticles with the lowest rate and the highest monodispersity. The presence of elemental silver in all prepared samples was confirmed using EDX, while the crystalline nature of synthesized particles was verified by XRD. FTIR results showed the presence of functional groups which reduce Ag(+) and stabilize AgNPs. The presence of nitrate reductase was confirmed in the cell-free filtrate of the fungus suggesting the potential role of this enzyme in AgNP synthesis. Synthesized particles showed significant antibacterial activity against E. coli as confirmed by examining the growth curve of bacterial cells exposed to AgNPs.

  6. Isolation, characterization and HPLC quantification of compounds from Aquilegia fragrans Benth: Their in vitro antibacterial activities against bovine mastitis pathogens.

    Science.gov (United States)

    Mushtaq, Saleem; Aga, Mushtaq A; Qazi, Parvaiz H; Ali, Md Niamat; Shah, Aabid Manzoor; Lone, Sajad Ahmad; Shah, Aiyatullah; Hussain, Aehtesham; Rasool, Faheem; Dar, Hafizullah; Shah, Zeeshan Hamid; Lone, Shabir H

    2016-02-03

    The underground parts of Aquilegia fragrans are traditionally used for the treatment of wounds and various inflammatory diseases like bovine mastitis. However, there are no reports on the phytochemical characterization and antibacterial studies of A. fragrans. To isolate compounds from the methanol extract of the underground parts of A. fragrans and determine their antibacterial activity against the pathogens of bovine mastitis. The study was undertaken in order to scientifically validate the traditional use of A. fragrans. Five compounds were isolated from the methanol extract of the underground parts of A. fragrans using silica gel column chromatography. Structural elucidation of the isolated compounds was done using spectral data analysis and comparison with literature. High performance liquid chromatography (HPLC) was used for the qualitative and quantitative determination of isolated compounds in the crude methanol extract. The methanol extract and isolated compounds were evaluated for antibacterial activities against mastitis pathogens using broth micro-dilution technique. The five isolated compounds were identified as (1) 2, 4-dihydroxyphenylacetic acid methyl ester (2) β-sitosterol (3) Aquilegiolide (4) Glochidionolactone-A and (5) Magnoflorine. A quick and sensitive HPLC method was developed for the first time for qualitative and quantitative determination of four isolated marker compounds from A. fragrans. The crude methanol extract and compound 5 exhibited weak antibacterial activities that varied between the bacterial species (MIC=500-3000 µg/ml). The above results show that the crude methanol extract and isolated compounds from A. fragrans exhibit weak antibacterial activities. Further phytochemical and pharmacological studies are required for proper scientific validation of the folk use of this plant species in the treatment of various inflammatory diseases like bovine mastitis. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  7. Synthesis, characterization, crystal structure and antibacterial activity of new sulfur-bridged dinuclear silver(I) thiosemicarbazone complex [Ag.sub.2./sub.(PPh.sub.3./sub.).sub.2./sub.(μ-S-Brcatsc).sub.2./sub.(η.sup.1./sup.-S-Brcatsc).sub.2./sub.](NO.sub.3./sub.).sub.2./sub..

    Czech Academy of Sciences Publication Activity Database

    Shahsavani, E.; Khalaji, A.D.; Feizi, N.; Kučeráková, Monika; Dušek, Michal

    2015-01-01

    Roč. 429, Apr (2015), 61-66 ISSN 0020-1693 R&D Projects: GA ČR(CZ) GA14-03276S Institutional support: RVO:68378271 Keywords : sulfur-bridged dinuclear silver (I) * thiosemicarbazone complex * single-crystal * distorted tetrahedron * antibacterial activity Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.918, year: 2015

  8. Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates.

    Science.gov (United States)

    Ali, Khursheed; Ahmed, Bilal; Dwivedi, Sourabh; Saquib, Quaiser; Al-Khedhairy, Abdulaziz A; Musarrat, Javed

    2015-01-01

    A simple and rapid microwave assisted method of green synthesis of silver nanoparticles (AgNPs) was developed using aqueous leaf extract of Eucalyptus globulus(ELE), and their antibacterial and antibiofilm potential investigated. With this aim, the aqueous solutions of ELE and AgNO3(1 mM) were mixed (1:4 v/v), and microwave irradiated at 2450 Mhz, for 30 sec. The instant color change of the ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. The intensity of peak at 428 nm in UV-Vis spectra, due to the surface plasmon resonance of AgNPs, varied with the amount of ELE, AgNO3 concentration, pH and time of incubation. The biosynthesized ELE-AgNPs were characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR and TGA analyses. The size of ELE-AgNPs was determined to be in range of 1.9-4.3 nm and 5-25 nm, with and without microwave treatment, respectively. SEM exhibited the capping of AgNPs with the ELE constituents, and validated by FTIR analysis. The FTIR data revealed the presence of plant organic constituents and metabolites bound to ELE-AgNPs, which contributes for their stability. The antimicrobial activity of ELE-AgNPs was assessed by growth and biofilm inhibition of extended spectrum β-lactamase (ESBL) producing Pseudomonas aeruginosa, Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) clinical bacterial isolates. The results demonstrated that S. aureus were more sensitive to ELE-AgNPs than E. coli and P. aeruginosa. MRSA exhibited higher sensitive than MSSA, whereas P. aeruginosa were more sensitive than E. coli to ELE-AgNPs treatment. Also, significant (83 ± 3% and 84 ± 5%) biofilm inhibition was observed in case of S. aureus and P. aeruginosa, respectively. The results elucidated environmentally friendly, economical and quick method for production of colloidal bio-functionalized ELE-AgNPs, for effectual clinical applications, as broad spectrum

  9. Microwave Accelerated Green Synthesis of Stable Silver Nanoparticles with Eucalyptus globulus Leaf Extract and Their Antibacterial and Antibiofilm Activity on Clinical Isolates.

    Directory of Open Access Journals (Sweden)

    Khursheed Ali

    Full Text Available A simple and rapid microwave assisted method of green synthesis of silver nanoparticles (AgNPs was developed using aqueous leaf extract of Eucalyptus globulus(ELE, and their antibacterial and antibiofilm potential investigated. With this aim, the aqueous solutions of ELE and AgNO3(1 mM were mixed (1:4 v/v, and microwave irradiated at 2450 Mhz, for 30 sec. The instant color change of the ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. The intensity of peak at 428 nm in UV-Vis spectra, due to the surface plasmon resonance of AgNPs, varied with the amount of ELE, AgNO3 concentration, pH and time of incubation. The biosynthesized ELE-AgNPs were characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR and TGA analyses. The size of ELE-AgNPs was determined to be in range of 1.9-4.3 nm and 5-25 nm, with and without microwave treatment, respectively. SEM exhibited the capping of AgNPs with the ELE constituents, and validated by FTIR analysis. The FTIR data revealed the presence of plant organic constituents and metabolites bound to ELE-AgNPs, which contributes for their stability. The antimicrobial activity of ELE-AgNPs was assessed by growth and biofilm inhibition of extended spectrum β-lactamase (ESBL producing Pseudomonas aeruginosa, Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA and methicillin-sensitive Staphylococcus aureus (MSSA clinical bacterial isolates. The results demonstrated that S. aureus were more sensitive to ELE-AgNPs than E. coli and P. aeruginosa. MRSA exhibited higher sensitive than MSSA, whereas P. aeruginosa were more sensitive than E. coli to ELE-AgNPs treatment. Also, significant (83 ± 3% and 84 ± 5% biofilm inhibition was observed in case of S. aureus and P. aeruginosa, respectively. The results elucidated environmentally friendly, economical and quick method for production of colloidal bio-functionalized ELE-AgNPs, for effectual clinical applications, as broad

  10. Synthesis, crystal structure, antibacterial activities, and electrochemical studies of new N,N‧-polymethylene bis-sulfonamides

    Science.gov (United States)

    Özbek, Neslihan; Alyar, Saliha; Mamaş, Serhat; Şahin, Ertan; Karacan, Nurcan

    2012-02-01

    Four disulfonamide derivatives (C2H5·SO2·NH)2(CH2)n (n = 2, 3, 4, 5) were synthesized and characterized by FTIR, 1H NMR, 13C NMR, HETCOR, LCMS and elemental analysis. Ethanesulfonamide-N,N'-pentamethylene bis was also characterized by X-ray single crystal diffraction measurement. The electrochemical characteristics of the disulfonamide derivatives were performed by cyclic voltammetry and chronoamperometry. 1H and 13C NMR chemical shifts of the compounds were calculated by using DFT/B3LYP methods with a 6-311++G (d,p) basis set. Antibacterial activity and the structural relationship of the compounds showed that activity decreases proportionately to the increasing length of the carbon chain between NH groups, log P values, hydration energy and molecular volumes. Anodic peak potentials and HOMO values do not correlate with the activity, but reduction potential and LUMO decrease weakly with increasing activity.

  11. Polypyrrole-silver Nanocomposite: Synthesis and Characterization

    Directory of Open Access Journals (Sweden)

    D. M. Nerkar

    2016-07-01

    Full Text Available Polypyrrole-Silver (PPy-Ag nanocomposite has been successfully synthesized by the chemical oxidative polymerization of pyrrole with iron (III chloride as an oxidant, in the presence of a colloidal suspension of silver nanoparticles. Turkevich method (Citrate reduction method was used for the synthesis of silver nanoparticles (Ag NPs. The silver nanoparticles were characterized by UV-Visible spectroscopy which showed an absorption band at 423 nm confirming the formation of nanoparticles. PPy-Ag nanocomposite was characterized by Transmission Electron Microscopy (TEM, Scanning Electron Microscopy (SEM, Fourier Transform Infrared Spectroscopy (FTIR and X-ray diffraction (XRD techniques for morphological and structural confirmations. TEM and SEM images revealed that the silver nanoparticles were well dispersed in the PPy matrix. XRD pattern showed that PPy is amorphous but the presence of the peaks at 2q values of 38.24°, 44.57°, 64.51° and 78.45° corresponding to a cubic phase of silver, revealed the incorporation of silver nanoparticles in the PPy matrix. A possible formation mechanism of PPy-Ag nanocomposite was also proposed. The electrical conductivity of PPy-Ag nanocomposite was studied using two probe method. The electrical conductivity of the PPy-Ag nanocomposite prepared was found to be 4.657´10- 2 S/cm, whereas that of pure PPy was found to be 9.85´10-3 S/cm at room temperature (303 K. The value of activation energy (Ea for pure PPy was 0.045 eV while it decreased to 0.034 eV for PPy-Ag nanocomposite. The synthesized nanocomposite powder can be utilized as a potential material for fabrication of gas sensors operating at room temperature.

  12. Preparation and Characterization of Nylon 6/Silver Nanocomposite Fibers for Permanent Antibacterial Effect

    OpenAIRE

    Laleh Maleknia; A. Saeed Rashidi

    2015-01-01

    The nylon 6/silver nano composite fibers were prepared for the attainment of permanent antibacterial activity to common synthetic textile. The fibers prepared by melt spinning and nylon 6/ Silver nanocomposite were prepared by a modular twin screw extruder.. The antibacterial activities of nano silver in fibers were calculated by percent reduction of two kinds of bacteria staphylococcus aurous and klebsiela peneumoniae. Scanning electron microscopy (SEM) was carried out to observe particle di...

  13. Production and characterization of a new antibacterial peptide obtained from Aeribacillus pallidus SAT4

    OpenAIRE

    Muhammad, Syed Aun; Ahmed, Safia

    2015-01-01

    A novel thermophilic bacterial strain of the genus Aeribacillus was isolated from Thar Dessert Pakistan. This strain showed significant antibacterial activity against Micrococcus luteus, Staphylococcus aureus, and Pseudomonas aeruginosa. The strain coded as ‘SAT4’ resembled with Aeribacillus pallidus in the morphological, biochemical and molecular tests. The production of antibacterial metabolites by SAT4 was optimized. These active metabolites were precipitated by 50% ammonium sulphate and p...

  14. Synthesis and characterization of functional magnetic nanocomposites

    Science.gov (United States)

    Gass, J.; Sanders, J.; Srinath, S.; Srikanth, H.

    2006-03-01

    Magnetic nanoparticles and carbon nanotubes have been excellent functional materials that could be dispersed in polymer matrices for various applications. However, uniform dispersion of particles in polymers without agglomeration is quite challenging. We have fabricated PMMA/polypyrrole bilayer structures embedded with Fe3O4 magnetite nanoparticles synthesized using wet chemical synthesis. Agglomeration-free dispersion of nanoparticles was achieved by coating the particles with surfactants and by dissolving both the particles and PMMA in chlorobenzene. Structural characterization was done using XRD and TEM. Magnetic properties of the bilayer structures indicated superparamagnetic behavior that is desirable for RF applications as the magnetic losses are reduced. Our polymer nanocomposite bilayer films with conducting polymer coatings are potential candidates for tunable RF applications with integrated EMI suppression. We will also report on our studies of pumped ferrofluids flowing past carbon nanotubes that are arranged in microchannel arrays. Magnetization under various flow conditions is investigated and correlated with the hydrodynamic properties. This scheme provides a novel method of energy conversion and storage using nanocomposite materials.

  15. Synthesis and characterization of cuprate superconductors

    International Nuclear Information System (INIS)

    Schaeffer, R.W. III.

    1992-01-01

    Superconducting powders and films were synthesized by a variety of methods and solvent systems: chemical solidification, freeze drying, and spray pyrolysis from livid ammonia (to form powders and films); reactions in molten sodium hydroxide/sodium peroxide and sodium nitrate/potassium nitrate mixtures (to form powders); and gel formation, coprecipitation, and spray drying from aqueous/organic mixtures (to form powders and films). These materials were characterized for elemental content and phase purity by gravimetric and volumetric analysis, atomic absorption spectroscopy, x-ray fluorescence and x-ray diffraction techniques. Particle size and surface morphology were determined by scanning electron microscopy and x-ray diffraction analysis. Also, precursor reactions were followed as a function of temperature with thermal gravimetric analysis and differential scanning calorimetry. Finally, physical properties determined for the resulting superconducting phases included resistivity, magnetic susceptibility, critical current, and percent Meissner effect. These results are discussed as a function of process parameters with particular attention to the role of atomic level mixing in solid state synthesis

  16. Synthesis and Characterization of 2-D Materials

    Science.gov (United States)

    Pazos, S.; Sahoo, P.; Afaneh, T.; Rodriguez Gutierrez, H.

    Atomically thin transition-metal dichacogenides (TMD), graphene, and boron nitride (BN) are two-dimensional materials where the charge carriers (electrons and holes) are confined to move in a plane. They exhibit distinctive optoelectronic properties compared to their bulk layered counterparts. When combined into heterostructures, these materials open more possibilities in terms of new properties and device functionality. In this work, WSe2 and graphene were grown using Chemical Vapor Deposition (CVD) and Physical Vapor Deposition (PVD) techniques. The quality and morphology of each material was checked using Raman, Photoluminescence Spectroscopy, and Scanning Electron Microscopy. Graphene had been successfully grown homogenously, characterized, and transferred from copper to silicon dioxide substrates; these films will be used in future studies to build 2-D devices. Different morphologies of WSe2 2-D islands were successfully grown on SiO2 substrates. Depending on the synthesis conditions, the material on each sample had single layer, double layer, and multi-layer areas. A variety of 2-D morphologies were also observed in the 2-D islands. This project is supported by the NSF REU Grant #1560090 and NSF Grant #DMR-1557434.

  17. Synthesis and characterization of novel nanothermometers

    Energy Technology Data Exchange (ETDEWEB)

    Baumert, Delphine [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Larsen, George [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Coopersmith, Kaitlin [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Schyck, Sarah [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Murph, Simona [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-03-23

    A straightforward approach was developed for the synthesis of Pd, Pd-Fe2O3, Au-Fe2O3, and Au-Pd-Fe2O3 nanothermometers, using a single SL DNA. These NP-DNA conjugates were characterized using techniques including EDX measurements, ζ-potential of NPs before and after DNA functionalization, electron microscopy studies and fluorescence spectroscopy. The fluorescence studies of the NP-DNA demonstrate the interaction between the NP and the fluorophore, which is quenched in the case of Au-Pd-Fe2O3 NPs and is perhaps enhanced (when compared to AuNPs) in the case of Pd and Pd-Fe2O3 NPs. In order to achieve more accurate and reproducible measurements, designing a system that is able to hold the NP-DNA conjugates at a temperature for a longer period of time to allow them to 12 equilibrate is currently underway. Our studies show that Au-Pd-Fe2O3 NPs are the best candidate material to serve as nanothermometers when compared to Pd, Pd-Fe2O3, and Au-Fe2O3 materials.

  18. Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

    Science.gov (United States)

    Azizi, Susan; Mahdavi Shahri, Mahnaz; Rahman, Heshu Sulaiman; Rahim, Raha Abdul; Rasedee, Abdullah; Mohamad, Rosfarizan

    2017-01-01

    Among nanoparticles used for medical applications, palladium nanoparticles (PdNPs) are among the least investigated. This study was undertaken to develop PdNPs by green synthesis using white tea (W.tea; Camellia sinensis) extract to produce the Pd@W.tea NPs. The Pd@W.tea NPs were characterized by UV–vis spectroscopy and X-ray diffractometry, and evaluated with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The Pd@W.tea NPs were spherical (size 6–18 nm) and contained phenols and flavonoids acquired from the W.tea extract. Pd@W.tea NPs has good 1-diphenyl-2-picrylhydrazyl (DPPH), OH, and NO-scavenging properties as well as antibacterial effects toward Staphylococcus epidermidis and Escherichia coli. MTT assay showed that Pd@W.tea NPs (IC50 =0.006 μM) were more antiproliferative toward the human leukemia (MOLT-4) cells than the W.tea extract (IC50 =0.894 μM), doxorubicin (IC50 =2.133 μM), or cisplatin (IC50 =0.013 μM), whereas they were relatively innocuous for normal human fibroblast (HDF-a) cells. The anticancer cell effects of Pd@W.tea NPs are mediated through the induction of apoptosis and G2/M cell-cycle arrest. PMID:29276385

  19. The Scaevola frutescen (Mill.) Krause dried-leaves extract as a potential natural reduction system for synthesis of gold nanoparticles and their evaluation for antibacterial of Eschericia coli

    Science.gov (United States)

    Salprima Yudha, S.; Angasa, Eka; Suharto, Totok Eka; Nishina, Yuta; Mardlia, Zulfikri Achid; Sipriyadi

    2016-02-01

    Many reports recently show that some plant and animal extracts were useful to prepare some desired materials. In line with the statement, this report will present a bioresources candidate that can be used for green preparation of gold nanoparticles. The Scaevola fruteschen (Mill.) Krause was obtained from Enggano island, Bengkulu Province, Indonesia. The current research include synthesis, characterization and application study of the obtained nanoparticles in their original medium/reduction system as antibacterial agent of Escherichia coli. Besides that, the interaction study of the nanoparticles solution with Pb2+ ions was also studied. The results, shows that the present reduction system (aqueous extract of the plant leaves) successfully reduced gold (III) ions to gold metals as shown by new peak at 544 nm in spectrophotometry analysis. The average nanopartiles size was 96 nm based on Particles Size Analyzer (PSA) result. The stability study revealed that the nanoparticles solution was not stable in the persence of Pb(OAc)2 at high concentration. The nanoparticles were effective enough toward E. coli as shown by inhibition zone that indicates the degree of sensitivity of bacteria to nanoparticles.

  20. Low-cost and eco-friendly green synthesis of silver nanoparticles using Prunus japonica (Rosaceae) leaf extract and their antibacterial, antioxidant properties.

    Science.gov (United States)

    Saravanakumar, Arthanari; Peng, Mei Mei; Ganesh, Mani; Jayaprakash, Jayabalan; Mohankumar, Murugan; Jang, Hyun Tae

    2017-09-01

    Low cost and eco-friendly green synthesis of silver nanoparticles (AgNPs) from silver nitrate (AgNO 3 ) using Prunus japonica leaves extract as reducing agent by a simple method at room temperature. The biosynthesized nanoparticles (NPs) were characterized by UV-Vis, tunneling electron microscopy (HR-TEM), scanning electron microscopy (SEM) coupled with X-ray energy dispersive spectrophotometer (EDAX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). In UV-Vis spectroscopy results, the λ max was observed at 441 nm. The AgNPs synthesized were spherical, hexagonal, and irregular in shapes. The EDAX and XRD spectrum confirmed the presence of silver ions and crystalline nature of synthesized AgNPs. FTIR showed the functional groups such as C = O, N-H and C-N groups involved in the reduction of Ag +  to Ag. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was performed and it showed the percentage inhibition in concentration-dependent manner. The synthesized AgNPs showed antibacterial activity against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus to different extents and the higher activity was observed in Proteus vulgaris.

  1. Eco-friendly green synthesis of silver nanoparticles using salmalia malabarica: synthesis, characterization, antimicrobial, and catalytic activity studies

    Science.gov (United States)

    Murali Krishna, I.; Bhagavanth Reddy, G.; Veerabhadram, G.; Madhusudhan, A.

    2016-06-01

    An economically viable and "green" process has been developed for the synthesis of silver nanoparticles (AgNPs) with an average size of 7 nm using non-toxic and renewable salmalia malabarica gum (SMG) as reducing and capping agent without using any chemical reducing agent. The effect of various parameters such as concentration of SMG and silver nitrate and reaction time for the synthesis of AgNPs was studied. The synthesized AgNPs are systematically characterized by UV/Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Transmission electron microscopy. The resultant SMG-capped AgNPs are highly stable and had significant antibacterial action on both Escherichia coli ( E. coli) and Staphylococcus aureus ( S. aureus). The catalytic action of the SMG-capped AgNPs to initiate the reduction of 4-nitrophenol (4-NP) in the presence of NaBH4 has also been reported. The kinetics of the reaction was found to be of pseudo-first-order with respect to the 4-NP.

  2. SYNTHESIS AND CHARACTERIZATION OF IRON (II) AND NICKEL ...

    African Journals Online (AJOL)

    DR. AMINU

    SYNTHESIS AND CHARACTERIZATION OF IRON (II) AND NICKEL (II). SCHIFF BASE COMPLEXES. * Aliyu, H. N. and A. S. ... synthesis and magnetic studies on schiff base complexes of copper (II). Recently, Xishi et al. (2003) .... pyridylmethanimines as Tuneable Alternatives to Bipyridine Ligan in Copper Medicated Atom.

  3. Synthesis and characterization of six-membered pincer ...

    Indian Academy of Sciences (India)

    0013167

    SUPPORTING INFORMATION. REGULAR ARTICLE. Synthesis and characterization of six-membered pincer nickelacycles and application in alkylation of benzothiazole. †. HANUMANPRASAD PANDIRI,a DIPESH M SHARMA,a RAJESH G GONNADEb and. BENUDHAR PUNJI*,a. aOrganometallic Synthesis and Catalysis ...

  4. Eco-sustainable synthesis and biological evaluation of 2-phenyl 1,3-benzodioxole derivatives as anticancer, DNA binding and antibacterial agents

    Directory of Open Access Journals (Sweden)

    Sayan Dutta Gupta

    2016-11-01

    Full Text Available The current research and development scenario in medicinal chemistry demands small molecules synthesized in a simple, fast and effective way with enhanced activity and fewer side effects than the existing ones. Therefore, one-pot, microwave assisted green and efficient synthesis of a series of derivatives belonging to 2-phenyl 1,3-benzodioxole (1a–14a and 2-phenyl 1,3-benzodioxole-4-ol (1b–14b class were carried out and subsequently investigated for their anticancer, antibacterial and DNA binding potential. Compound 3c proved to be the most active one among the screened derivatives possessing anticancer and antibacterial potency greater than the standard reference compound (cisplatin and cinoxacin for anticancer and antibacterial activity, respectively. The most active compound in terms of DNA binding capacity was found to be 5b. A rewarding feature of the work is a facile, convenient, eco friendly one step synthesis of compounds demonstrating attenuated activity against cancer and bacterial cell with an inherent potential of binding to DNA. Subsequently, a hit molecule for further anticancer, antibacterial (compound 3c and DNA binding studies (compound 5b was also identified.

  5. Synthesis, antibacterial and cytotoxic activities of new biflorin-based hydrazones and oximes.

    Science.gov (United States)

    da S Souza, Luciana G; Almeida, Macia C S; Lemos, Telma L G; Ribeiro, Paulo R V; de Brito, Edy S; Silva, Vera L M; Silva, Artur M S; Braz-Filho, Raimundo; Costa, José G M; Rodrigues, Fábio F G; Barreto, Francisco S; de Moraes, Manoel O

    2016-01-15

    Biflorin 1 is a biologically active quinone, isolated from Capraria biflora. Five new biflorin-based nitrogen derivatives were synthesized, of which two were mixtures of (E)- and (Z)- isomers: (Z)-2a, (Z)-2b, (Z)-3a, (Z)- and (E)-3b, (Z)- and (E)-3c. The antibacterial activity was investigated using the microdilution method for determining the minimum inhibitory concentration (MIC) against six bacterial strains. Tests have shown that these derivatives have potential against all bacterial strains. The cytotoxic activity was also evaluated against three strains of cancer cells, but none of the derivatives showed activity. Copyright © 2015. Published by Elsevier Ltd.

  6. Synthesis, Anti-Bacterial and Anti-Fungal Evaluation of Pyrazoline Derivatives

    Directory of Open Access Journals (Sweden)

    Ashvin D. Panchal

    2012-01-01

    Full Text Available The Series of N-(5-(Substituted phenyl-4,5-dihydro-1H-pyrazol-3-yl-4H-1,2,4-triazol-4-amine compounds were prepared by reaction of 4-amino-1,2,4-Triazole with Acetyl Chloride followed by different aromatic aldehydes and cyclization with hydrazine hydrate. The structures of new compounds were confirmed by IR and 1H-NMR spectral data. Anti-bacterial and Anti-fungal activities were evaluated and compared with the standard drugs, some compounds of the series exhibited promising anti-microbial and anti-fungal activity compared to standard drugs.

  7. Characterization of d-boroAla as a Novel Broad Spectrum Antibacterial Agent Targeting d-Ala-d-Ala Ligase

    OpenAIRE

    Putty, Sandeep; Rai, Aman; Jamindar, Darshan; Pagano, Paul; Quinn, Cheryl L.; Mima, Takehiko; Schweizer, Herbert P.; Gutheil, William G.

    2011-01-01

    d-boroAla was previously characterized as an inhibitor of bacterial alanine racemase and d-Ala-d-Ala ligase enzymes [Duncan, K., et al Biochemistry 1989, 28:3541–9]. In the present study, d-boroAla was identified and characterized as an antibacterial agent. d-boroAla has activity against both Gram-positive and Gram-negative organisms, with MICs down to 8 µg/mL. A structure-function study on the alkyl side chain (NH2-CHR-B(OR’)2) revealed that d-boroAla is the most effective agent in a series ...

  8. The nitro-reduced metabolite of nimesulide: Crystal structure, spectroscopic characterization, ESI-QTOF mass spectrometric analysis and antibacterial evaluation

    Science.gov (United States)

    Nunes, Julia H. B.; Nakahata, Douglas H.; Lustri, Wilton R.; Corbi, Pedro P.; de Paiva, Raphael E. F.

    2018-04-01

    Here we present a synthetic procedure, spectroscopic characterization and single-crystal X-ray structure for the nitro-reduced metabolite of the anti-inflammatory drug nimesulide, hereby referred to as NMS-NH2. The nitro-reduced metabolite was synthesized using the Béchamp reduction (iron powder under acidic media), leading to the conversion of the nitrobenzene group of nimesulide to an aniline. Mass spectrometry, infrared and nuclear magnetic resonance spectroscopies data are also provided for NMS-NH2, and discussed in comparison to nimesulide. NMS-NH2 was also evaluated in terms of its antibacterial activities, considering that the free sbnd NH2 group could allow the compound to act as a dihydropteroate synthase inhibitor. NMS-NH2 had a modest antibacterial activity against P. aeruginosa (5.0 mg mL-1), which was not observed for NMS.

  9. Facile fabrication and characterization of poly(tetrafluoroethylene)@polypyrrole/nano-silver composite membranes with conducting and antibacterial property

    Science.gov (United States)

    Shi, Zhiquan; Zhou, Hui; Qing, Xutang; Dai, Tingyang; Lu, Yun

    2012-06-01

    Porous poly(tetrafluoroethylene) (PTFE) membranes play an important role in air purification and separation engineering. To achieve the bi-functionality of conducting and antibacterial property, two kinds of poly(tetrafluoroethylene)@ polypyrrole/nano-silver composite membranes have been prepared. One involves hydrophobic polypyrrole/nano-silver composite with hollow capsule nanostructures immobilized on the surface of the PTFE membranes. The other is a type of composite membranes with polypyrrole/nano-silver composite wholly packed on the fibrils of the expand PTFE membrane to form core/shell coaxial cable structures. The structure and morphology of the two kinds of composite membranes have been characterized by FTIR, UV-vis, XRD, TGA and SEM measurements. Possible formation mechanisms of the hollow capsules and the core/shell nanocable structures have been discussed in detail. The antibacterial effects of composite membranes are also briefly investigated.

  10. Preparation and characterization of antibacterial electrospun chitosan/poly (vinyl alcohol)/graphene oxide composite nanofibrous membrane

    Science.gov (United States)

    Yang, Shuai; Lei, Peng; Shan, Yujuan; Zhang, Dawei

    2018-03-01

    In this paper, chitosan (CS)/poly (vinyl alcohol) (PVA)/graphene oxide (GO) composite nanofibrous membranes were prepared via electrospinning. Such nanofibrous membranes have been characterized and investigated for their morphological, structural, thermal stability, hydrophilic and antibacterial properties. SEM images showed that the uniform and defect-free nanofibers were obtained and GO sheets, shaping spindle and spherical, were partially embedded into nanofibers. FTIR, XRD, DSC and TGA indicated the good compatibility between CS and PVA. There were strong intermolecular hydrogen bonds between the chitosan and PVA molecules. Contact angle measurement indicated that while increasing the content of GO, the distance between fibers increased and water drop showed wetting state on the surface of nanofibrous membranes. As a result, the contact angle decreased significantly. Meanwhile, good antibacterial activity of the prepared nanofibrous membranes were exhibited against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus.

  11. Synthesis and characterization of amorphous cellulose from triacetate of cellulose

    International Nuclear Information System (INIS)

    Vega-Baudrit, Jose; Sibaja, Maria; Nikolaeva, Svetlana; Rivera A, Andrea

    2014-01-01

    It was carried-out a study for the synthesis and characterization of amorphous cellulose starting from cellulose triacetate. X-rays diffraction was used in order to obtain the cellulose crystallinity degree, also infrared spectroscopy FTIR was used. (author)

  12. Synthesis, spectroscopic and DFT characterization of 4 β -(4- tert ...

    African Journals Online (AJOL)

    Synthesis, spectroscopic and DFT characterization of 4 β -(4-tert-butylphenoxy) phthalocyanine positional isomers for non-linear optical absorption. Denisha Gounden, Grace N. Ngubeni, Marcel S. Louzada, Samson Khene, Jonathan Britton, Nolwazi Nombona ...

  13. Gold (I)-selenolate complexes: Synthesis, characterization and ...

    Indian Academy of Sciences (India)

    selenolate complexes: Synthesis, characterization and ligand exchange reactions. Krishna P Bhabak ... This study indicates that the nature of selenolate plays an important role in ligand exchange reactions in gold(I) selenolates. Furthermore, the ...

  14. Synthesis, characterization and emission properties of quinolin-8 ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 116; Issue 5. Synthesis, characterization and emission properties of quinolin-8-olato chelated ruthenium organometallics. Bikash Kumar Panda. Volume 116 ... Keywords. Ruthenium organometallics; quinolin-8-olato chelation; emission properties; trivalent ruthenium.

  15. Synthesis and characterization of ceria nanomaterials

    Science.gov (United States)

    Cheong Ng, Nitzia

    Cerium dioxide or ceria, CeO2, has been widely used in industry as catalyst for automotive exhaust controls, chemical mechanical polishing (CMP) slurries, and high temperature fuel cells because of its unique metal oxide properties. This well-known rare metal oxide has high thermal stability, electrical conductivity and chemical diffusivity. Proper synthesis method requires knowledge of reaction temperature, concentration, and time effects on the synthesis. In this work, ceria nanomaterials were prepared via the hydrothermal method using a Teflon autoclave. Cerium nitrate solution was used as the source and three different precursors: NaOH, H2O 2, and NH4OH were used as the oxidizing agents. CeO 2 nanoplates, nanocubes and nanorods were produced and studied using transmission electron microscopy (TEM), BET specific surface area, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Through characterization, CeO2 nanomaterials showed the presence of mixed valence states (Ce3+ and Ce4+) through XPS spectra. Deconvolution was performed to investigate the ratio of Ce 3+/Ce4+ concentration in the synthesized CeO2 nanostructures. Nanocubes showed a higher Ce3+ concentration. CeO2 nanomaterials were found to be mesoporous. Nanoplates synthesized with H2O2, and NH4OH were found with surface areas of 95.11 m2/g and 62.07 m2/g, respectively. Nanorods and nanocubes showed surface areas of 16.77 m2/g and 16.55 m2/g, respectively. The prepared ceria nanoplates, nanocubes and nanorods had crystallite size in the range of 5--25 nm and pore size range of 7--15 nm. XRD spectra confirmed that the peaks were indexed to the cubic phase of CeO2 with fluorite structure and with an average lattice parameter, 5.407 A. Higher Ce3+ concentration and exposed surface of crystalline planes suggest that nanorods are better catalyst for CO oxidation and oxygen storage capacity (OSC).

  16. Synthesis and characterization of hydroxyapatite-doped silver nanoparticles

    International Nuclear Information System (INIS)

    Andrade, Flavio Augusto Cavadas da Silva; Rollo, Joao Manuel Domingos de Almeida; Rigo, Eliana Cristina da Silva; Vercik, Andres; Vercik, Luci Cristina de Oliveira; Valencia, German Ayala; Ferreira, Leticcia Gaviao

    2012-01-01

    Hydroxyapatite-doped silver nanoparticles was obtained by immersing the powder in increasing dilutions of a solution containing AGNPS which were synthesized in different times and were characterized by UV-vis spectroscopy. The X-ray diffraction (XRD)studies demonstrate no change in the major phase of HA. Scanning Electron Microscopy (SEM) revealed morphological characteristics of powders after doping and the presence of silver was confirmed by energy dispersive X-ray (EDAX) analysis.The antibacterial effect of the doped powders was evaluated using strain of Staphylococcus aureus by disc-diffusion test. The zone of inhibition was found to vary with the amount of silver nanoparticle in the doped powder even for low concentrations of AgNPs. These results indicate that the method of immersion hydroxyapatite in solutions containing AgNPs is promising to obtain bioactive materials with low cytotoxicity and antibacterial effects. (author)

  17. Novel microwave-synthesis of Cu nanoparticles in the absence of any stabilizing agent and their antibacterial and antistatic applications

    Energy Technology Data Exchange (ETDEWEB)

    Raspolli Galletti, Anna Maria, E-mail: roxy@dcci.unipi.it [Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Via Risorgimento 35, 56126 Pisa (Italy); Antonetti, Claudia, E-mail: claudia.antonetti@ns.dcci.unipi.it [Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Via Risorgimento 35, 56126 Pisa (Italy); Marracci, Mirko, E-mail: mirko.marracci@ing.unipi.it [Dipartimento di Ingegneria dell’Energia, dei Sistemi, del Territorio e delle, Costruzioni, Università di Pisa, via Diotisalvi 2, 56125 Pisa (Italy); Piccinelli, Fabio, E-mail: fabio.piccinelli@univr.it [Laboratorio di Chimica dello Stato Solido, DB, Università di Verona, and INSTM, UdR Verona, Strada le Grazie 15, 37134 Verona (Italy); Tellini, Bernardo, E-mail: bernardo.tellini@ing.unipi.it [Dipartimento di Ingegneria dell’Energia, dei Sistemi, del Territorio e delle, Costruzioni, Università di Pisa, via Diotisalvi 2, 56125 Pisa (Italy)

    2013-09-01

    For the first time, copper nanoparticles were synthesized under microwave (MW) irradiation in the absence of any stabilizing agent. A 2-step synthetic approach was adopted working in basic ethanol solution and then ascorbic acid was added as a reducing reagent in the second step. The obtained copper nanoparticles were characterized by UV–vis spectroscopy, XRPD and TEM analysis. UV–vis spectra show an absorption peak at about 580–590 nm, typical of the plasma resonance of copper nanoparticles and XRPD analysis reveals that the complete reduction to metallic copper was reached at the end of the second step. Average sizes in the range 7–15 nm were ascertained through TEM microscopy. These copper nanoparticles are suitable for antibacterial and antistatic applications. The bactericidal effect was investigated in relation to the diameter of inhibition zone in disk diffusion tests on calf crust leather sample and an interesting antibacterial activity was verified against both Gram positive and Gram negative bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans). Moreover, this treated leather showed encouraging antistatic behavior: in particular, equivalent circuital parameters were estimated via an impedance spectroscopy technique to have a first evaluation of the charge dissipation activity by volume conduction.

  18. Novel enaminones as non-cytotoxic compounds with mild antibacterial activity: Synthesis and structure-activity correlations

    Science.gov (United States)

    Cindrić, Marina; Rubčić, Mirta; Hrenar, Tomica; Pisk, Jana; Cvijanović, Danijela; Lovrić, Jasna; Vrdoljak, Višnja

    2018-02-01

    Six non-symmetric enaminones 4-[(2-hydroxy-5-methylphenyl)amino]pent-3-en-2-one (H2L1), 4-[(2-hydroxy-4-methylphenyl)amino]pent-3-en-2-one (H2L2), 4-[(4-hydroxy-2-methylphenyl)amino)]pent-3-en-2-one (H2L3), 3-[(2-hydroxy-5-methylphenyl)amino]-1-phenylbut-2-en-1-one (H2L4), 3-[(2-hydroxy-4-methylphenyl)amino]-1-phenylbut-2-en-1-one (H2L5) and 3-[(4-hydroxy-2-methylphenyl)amino]-1-phenylbut-2-en-1-one (H2L6) have been prepared by solution based method. The enaminones were characterized by elemental and DSC analysis, NMR and IR spectroscopy. Crystal and molecular structures of H2L1, H2L2, H2L4 and H2L6 were determined via single crystal X-ray analysis. The prepared enaminones were screened against THP-1 and HepG2 cells, and Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Moraxella catarrhalis bacteria to assess their cytotoxic and antibacterial activity, respectively. All compounds proved to be non-cytotoxic and showed mild or no antibacterial activity. Quantum mechanical calculations suggest that the presence of hydroxy group in ortho position, combined with the methyl group on the same aromatic ring, has a significant impact on the biological activities.

  19. Novel microwave-synthesis of Cu nanoparticles in the absence of any stabilizing agent and their antibacterial and antistatic applications

    Science.gov (United States)

    Raspolli Galletti, Anna Maria; Antonetti, Claudia; Marracci, Mirko; Piccinelli, Fabio; Tellini, Bernardo

    2013-09-01

    For the first time, copper nanoparticles were synthesized under microwave (MW) irradiation in the absence of any stabilizing agent. A 2-step synthetic approach was adopted working in basic ethanol solution and then ascorbic acid was added as a reducing reagent in the second step. The obtained copper nanoparticles were characterized by UV-vis spectroscopy, XRPD and TEM analysis. UV-vis spectra show an absorption peak at about 580-590 nm, typical of the plasma resonance of copper nanoparticles and XRPD analysis reveals that the complete reduction to metallic copper was reached at the end of the second step. Average sizes in the range 7-15 nm were ascertained through TEM microscopy. These copper nanoparticles are suitable for antibacterial and antistatic applications. The bactericidal effect was investigated in relation to the diameter of inhibition zone in disk diffusion tests on calf crust leather sample and an interesting antibacterial activity was verified against both Gram positive and Gram negative bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans). Moreover, this treated leather showed encouraging antistatic behavior: in particular, equivalent circuital parameters were estimated via an impedance spectroscopy technique to have a first evaluation of the charge dissipation activity by volume conduction.

  20. Synthesis, anticancer and antibacterial evaluation of novel (isopropylidene uridine-[1,2,3]triazole hybrids

    Directory of Open Access Journals (Sweden)

    Ranjith kumar Thatipamula

    2017-11-01

    Full Text Available A series of novel (isopropylidene uridine-[1,2,3]triazole hybrids (3a–3n were efficiently synthesized via the copper-catalyzed azide–alkyne cycloaddition (CuAAC from N-propargyl 2′,3′-O-(isopropylidene uridine with different aryl azides. All the synthesized compounds were screened for their in vitro anticancer and antibacterial activities. The anticancer activity results revealed that compounds 3d and 3f have registered equipotent activity against MCF-7 and 3n has shown excellent activity against HeLa in comparison with the standard drug Cisplatin. Remaining compounds have shown moderate to good anticancer activity against MCF-7 and HeLa cell lines. The antibacterial activity screening results revealed that, compounds 3b and 3n have shown excellent inhibition against Escherichia coli and Bacillus subtilis, 3d against Proteus vulgaris, 3k against Staphylococcus aureus and 3l against S. aureus and B. subtilis have shown equipotent activity in comparison with the standard drug Streptomycin.